LOCUS       DAZGAP010000001        66564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGAP010000001 DAZGAP010000000
VERSION     DAZGAP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.001"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..591
                     /locus_tag="ACHLAM_000001"
     CDS             88..591
                     /locus_tag="ACHLAM_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO7851594.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(658..2115)
                     /gene="nuoN"
                     /locus_tag="ACHLAM_000002"
     CDS             complement(658..2115)
                     /gene="nuoN"
                     /locus_tag="ACHLAM_000002"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO7851595.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2122..3651)
                     /gene="nuoM"
                     /locus_tag="ACHLAM_000003"
     CDS             complement(2122..3651)
                     /gene="nuoM"
                     /locus_tag="ACHLAM_000003"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO7851596.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3815..5656)
                     /gene="nuoL"
                     /locus_tag="ACHLAM_000004"
     CDS             complement(3815..5656)
                     /gene="nuoL"
                     /locus_tag="ACHLAM_000004"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO7851597.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5653..5955)
                     /gene="nuoK"
                     /locus_tag="ACHLAM_000005"
     CDS             complement(5653..5955)
                     /gene="nuoK"
                     /locus_tag="ACHLAM_000005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO7851598.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5952..6506)
                     /gene="nuoJ"
                     /locus_tag="ACHLAM_000006"
     CDS             complement(5952..6506)
                     /gene="nuoJ"
                     /locus_tag="ACHLAM_000006"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO7851599.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6518..7060)
                     /gene="nuoI"
                     /locus_tag="ACHLAM_000007"
     CDS             complement(6518..7060)
                     /gene="nuoI"
                     /locus_tag="ACHLAM_000007"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO7851600.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7075..8052)
                     /gene="nuoH"
                     /locus_tag="ACHLAM_000008"
     CDS             complement(7075..8052)
                     /gene="nuoH"
                     /locus_tag="ACHLAM_000008"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO7851601.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8049..10775)
                     /gene="nuoG"
                     /locus_tag="ACHLAM_000009"
     CDS             complement(8049..10775)
                     /gene="nuoG"
                     /locus_tag="ACHLAM_000009"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO7851602.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10828..12165)
                     /gene="nuoF"
                     /locus_tag="ACHLAM_000010"
     CDS             complement(10828..12165)
                     /gene="nuoF"
                     /locus_tag="ACHLAM_000010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO7851603.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEVGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12162..12662)
                     /gene="nuoE"
                     /locus_tag="ACHLAM_000011"
     CDS             complement(12162..12662)
                     /gene="nuoE"
                     /locus_tag="ACHLAM_000011"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO7851604.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12665..14467)
                     /gene="nuoC"
                     /locus_tag="ACHLAM_000012"
     CDS             complement(12665..14467)
                     /gene="nuoC"
                     /locus_tag="ACHLAM_000012"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO7851605.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14561..15223)
                     /gene="nuoB"
                     /locus_tag="ACHLAM_000013"
     CDS             complement(14561..15223)
                     /gene="nuoB"
                     /locus_tag="ACHLAM_000013"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO7851606.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15239..15682)
                     /gene="nuoA"
                     /locus_tag="ACHLAM_000014"
     CDS             complement(15239..15682)
                     /gene="nuoA"
                     /locus_tag="ACHLAM_000014"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO7851607.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16312..17250)
                     /gene="lrhA"
                     /locus_tag="ACHLAM_000015"
     CDS             complement(16312..17250)
                     /gene="lrhA"
                     /locus_tag="ACHLAM_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO7851608.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18170..19387
                     /gene="alaA"
                     /locus_tag="ACHLAM_000016"
     CDS             18170..19387
                     /gene="alaA"
                     /locus_tag="ACHLAM_000016"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO7851609.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19471..20070
                     /gene="yfbR"
                     /locus_tag="ACHLAM_000017"
     CDS             19471..20070
                     /gene="yfbR"
                     /locus_tag="ACHLAM_000017"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7851610.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20129..21961)
                     /locus_tag="ACHLAM_000018"
     CDS             complement(20129..21961)
                     /locus_tag="ACHLAM_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO7851611.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22048..22698)
                     /gene="hxpA"
                     /locus_tag="ACHLAM_000019"
     CDS             complement(22048..22698)
                     /gene="hxpA"
                     /locus_tag="ACHLAM_000019"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO7851612.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(22709..23203)
                     /locus_tag="ACHLAM_000020"
     CDS             complement(22709..23203)
                     /locus_tag="ACHLAM_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO7851613.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23287..23742)
                     /gene="yfbV"
                     /locus_tag="ACHLAM_000021"
     CDS             complement(23287..23742)
                     /gene="yfbV"
                     /locus_tag="ACHLAM_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO7851614.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24080..25282
                     /gene="ackA"
                     /locus_tag="ACHLAM_000022"
     CDS             24080..25282
                     /gene="ackA"
                     /locus_tag="ACHLAM_000022"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO7851615.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25357..27501
                     /gene="pta"
                     /locus_tag="ACHLAM_000023"
     CDS             25357..27501
                     /gene="pta"
                     /locus_tag="ACHLAM_000023"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7851616.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27691..29211
                     /gene="yfcC"
                     /locus_tag="ACHLAM_000024"
     CDS             27691..29211
                     /gene="yfcC"
                     /locus_tag="ACHLAM_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO7851617.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29244..29786)
                     /gene="yfcD"
                     /locus_tag="ACHLAM_000025"
     CDS             complement(29244..29786)
                     /gene="yfcD"
                     /locus_tag="ACHLAM_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO7851618.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29844..30395)
                     /gene="yfcE"
                     /locus_tag="ACHLAM_000026"
     CDS             complement(29844..30395)
                     /gene="yfcE"
                     /locus_tag="ACHLAM_000026"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO7851619.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30451..31095)
                     /gene="yfcF"
                     /locus_tag="ACHLAM_000027"
     CDS             complement(30451..31095)
                     /gene="yfcF"
                     /locus_tag="ACHLAM_000027"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO7851620.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31231..31878
                     /gene="yfcG"
                     /locus_tag="ACHLAM_000028"
     CDS             31231..31878
                     /gene="yfcG"
                     /locus_tag="ACHLAM_000028"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO7851621.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31935..32297
                     /gene="folX"
                     /locus_tag="ACHLAM_000029"
     CDS             31935..32297
                     /gene="folX"
                     /locus_tag="ACHLAM_000029"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO7851622.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32318..33211
                     /locus_tag="ACHLAM_000030"
     CDS             32318..33211
                     /locus_tag="ACHLAM_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO7851623.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33259..34161)
                     /gene="rpnB"
                     /locus_tag="ACHLAM_000031"
     CDS             complement(33259..34161)
                     /gene="rpnB"
                     /locus_tag="ACHLAM_000031"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO7851624.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(34358..35131)
                     /gene="hisP"
                     /locus_tag="ACHLAM_000032"
     CDS             complement(34358..35131)
                     /gene="hisP"
                     /locus_tag="ACHLAM_000032"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO7851625.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35139..35855)
                     /locus_tag="ACHLAM_000033"
     CDS             complement(35139..35855)
                     /locus_tag="ACHLAM_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7851626.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(35852..36538)
                     /gene="hisQ"
                     /locus_tag="ACHLAM_000034"
     CDS             complement(35852..36538)
                     /gene="hisQ"
                     /locus_tag="ACHLAM_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO7851627.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36628..37410)
                     /gene="hisJ"
                     /locus_tag="ACHLAM_000035"
     CDS             complement(36628..37410)
                     /gene="hisJ"
                     /locus_tag="ACHLAM_000035"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO7851628.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(37631..38413)
                     /gene="argT"
                     /locus_tag="ACHLAM_000036"
     CDS             complement(37631..38413)
                     /gene="argT"
                     /locus_tag="ACHLAM_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO7851629.1"
                     /translation="MKKSILALSLLVGLSAVASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(38679..39248)
                     /gene="ubiX"
                     /locus_tag="ACHLAM_000037"
     CDS             complement(38679..39248)
                     /gene="ubiX"
                     /locus_tag="ACHLAM_000037"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO7851630.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39343..40860)
                     /gene="purF"
                     /locus_tag="ACHLAM_000038"
     CDS             complement(39343..40860)
                     /gene="purF"
                     /locus_tag="ACHLAM_000038"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO7851631.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(40897..41385)
                     /gene="cvpA"
                     /locus_tag="ACHLAM_000039"
     CDS             complement(40897..41385)
                     /gene="cvpA"
                     /locus_tag="ACHLAM_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO7851632.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(41644..42294)
                     /gene="dedD"
                     /locus_tag="ACHLAM_000040"
     CDS             complement(41644..42294)
                     /gene="dedD"
                     /locus_tag="ACHLAM_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO7851633.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(42284..43552)
                     /gene="folC"
                     /locus_tag="ACHLAM_000041"
     CDS             complement(42284..43552)
                     /gene="folC"
                     /locus_tag="ACHLAM_000041"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO7851634.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYTSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(43622..44536)
                     /gene="accD"
                     /locus_tag="ACHLAM_000042"
     CDS             complement(43622..44536)
                     /gene="accD"
                     /locus_tag="ACHLAM_000042"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO7851635.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(44692..45351)
                     /locus_tag="ACHLAM_000043"
     CDS             complement(44692..45351)
                     /locus_tag="ACHLAM_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7851636.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(45434..46246)
                     /gene="truA"
                     /locus_tag="ACHLAM_000044"
     CDS             complement(45434..46246)
                     /gene="truA"
                     /locus_tag="ACHLAM_000044"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO7851637.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46246..47259)
                     /locus_tag="ACHLAM_000045"
     CDS             complement(46246..47259)
                     /locus_tag="ACHLAM_000045"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7851638.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47325..48461)
                     /gene="pdxB"
                     /locus_tag="ACHLAM_000046"
     CDS             complement(47325..48461)
                     /gene="pdxB"
                     /locus_tag="ACHLAM_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO7851639.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48560..49555
                     /gene="flk"
                     /locus_tag="ACHLAM_000047"
     CDS             48560..49555
                     /gene="flk"
                     /locus_tag="ACHLAM_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO7851640.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49552..50730)
                     /locus_tag="ACHLAM_000048"
     CDS             complement(49552..50730)
                     /locus_tag="ACHLAM_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7851641.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(50995..52215)
                     /gene="fabB"
                     /locus_tag="ACHLAM_000049"
     CDS             complement(50995..52215)
                     /gene="fabB"
                     /locus_tag="ACHLAM_000049"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO7851642.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52374..54380
                     /gene="mnmC"
                     /locus_tag="ACHLAM_000050"
     CDS             52374..54380
                     /gene="mnmC"
                     /locus_tag="ACHLAM_000050"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO7851643.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLKDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54436..54714)
                     /locus_tag="ACHLAM_000051"
     CDS             complement(54436..54714)
                     /locus_tag="ACHLAM_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO7851644.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQKASQQ"
     gene            complement(54748..55296)
                     /gene="epmC"
                     /locus_tag="ACHLAM_000052"
     CDS             complement(54748..55296)
                     /gene="epmC"
                     /locus_tag="ACHLAM_000052"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO7851645.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55296..56105)
                     /locus_tag="ACHLAM_000053"
     CDS             complement(55296..56105)
                     /locus_tag="ACHLAM_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO7851646.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56105..56929)
                     /gene="mepA"
                     /locus_tag="ACHLAM_000054"
     CDS             complement(56105..56929)
                     /gene="mepA"
                     /locus_tag="ACHLAM_000054"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO7851647.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(56932..58017)
                     /gene="aroC"
                     /locus_tag="ACHLAM_000055"
     CDS             complement(56932..58017)
                     /gene="aroC"
                     /locus_tag="ACHLAM_000055"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO7851648.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58052..58984)
                     /gene="prmB"
                     /locus_tag="ACHLAM_000056"
     CDS             complement(58052..58984)
                     /gene="prmB"
                     /locus_tag="ACHLAM_000056"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7851649.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59150..59701
                     /gene="smrB"
                     /locus_tag="ACHLAM_000057"
     CDS             59150..59701
                     /gene="smrB"
                     /locus_tag="ACHLAM_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO7851650.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(59817..60659)
                     /locus_tag="ACHLAM_000058"
     CDS             complement(59817..60659)
                     /locus_tag="ACHLAM_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO7851651.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(60661..61185)
                     /locus_tag="ACHLAM_000059"
     CDS             complement(60661..61185)
                     /locus_tag="ACHLAM_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7851652.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            complement(61182..61652)
                     /locus_tag="ACHLAM_000060"
     CDS             complement(61182..61652)
                     /locus_tag="ACHLAM_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7851653.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            complement(61649..62155)
                     /locus_tag="ACHLAM_000061"
     CDS             complement(61649..62155)
                     /locus_tag="ACHLAM_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7851654.1"
                     /translation="MKRSCSVWAGTWLLMCCIGSDFAVAGSKSVNLSLRVLVDAPPPC
                     TVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQMRATTVVINGETVIS
                     TGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVKQSGTTLSAAEFNAS
                     ATIVVDYQ"
     gene            complement(62172..62921)
                     /locus_tag="ACHLAM_000062"
     CDS             complement(62172..62921)
                     /locus_tag="ACHLAM_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7851655.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPYLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEELSRKG"
     gene            complement(62941..65580)
                     /locus_tag="ACHLAM_000063"
     CDS             complement(62941..65580)
                     /locus_tag="ACHLAM_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO7851656.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVARTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDSTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            complement(65666..66232)
                     /locus_tag="ACHLAM_000064"
     CDS             complement(65666..66232)
                     /locus_tag="ACHLAM_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7851657.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGN
                     SKNYSLSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFK
                     AWLVGETAAVTPTPAPFETLTTFQITYL"
BASE COUNT        16533 a        18062 c        16615 g        15354 t
ORIGIN      
        1 gcggatccag aaaaagcaac atgcgcattt tattttcttt cgtctgtttt aagatgcgaa
       61 acatgaaatt caacggagga aaaataaatg ggaatgattg gctattttgc agaaattgac
      121 tcagaaaaga ttaaccaact gcttgagtct actaaaaaac ctttgatgga taatatacat
      181 gacacgcttt caggattacg ccgactggat attgataaac gctgggattt tttacacttt
      241 ggcctgacgg gtacatctgc atttgacccc gcgaagaatg acccgctaag tcgcgcagtg
      301 cttggtgaac acagcctaga agatggcata gatggcttcc tgggattgac ctggaatcag
      361 gagttagccg cgacaatcga ccgactggaa gctctggatc gaagtgagct gcgtaaacag
      421 ttttccatta agcgactcaa tgaaatggag atttatccgg gagtaacctt ttcagaagag
      481 ttagaggggc aactctttgc ttcgatcatg cttgatatgg aaaagctgat tagcgcgtat
      541 cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag
      601 ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg acttacaaac tgactgctta
      661 catcagcggc attgctaacc gcacaatgct aatcagcggt tgtggccata cacccagcac
      721 cagtaccaac agtgcagaga tcagcaccac gataccgcct gcgctgtact gccagtttga
      781 tggtgcatcg cgacccggtt gttccggagc gtgaaggtat aagctcaccg ccacgcgcag
      841 gtagtagtag aggccgattg ccgaaccgac aactacggca cccaccagcc accacaagtg
      901 tgcctggaca ccgaccgcca gcacgtagaa cttaccgata aagcccagcg tcattgggat
      961 accagccagt gaaagcatca tcaccgtcat cactgccgcg aggatcggac gatgccagaa
     1021 cagaccgcgg taagagaaca gggaatcagc atccgggcca cgatacgggc tggacatcag
     1081 gctgaccacg ccgaacgcgc cgaggctgct gaacagataa cctgccaggt aaacccctac
     1141 cgcttccatc gacatctcgc cagtttgcag cgcaatcagc gccaccagca gatagccgag
     1201 gtgagagata gatgagtaac cgagcagacg tttgatattg gtctggctca gcgccatcag
     1261 gttaccgaag atgatggagg caaaggcgat aatcgccagc accacgcgaa tcgcttcgct
     1321 gtcacccacc ggtgcgtaga ggaacagacg catcaccaca ccgaagatag cgattttgct
     1381 cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca
     1441 caggtggaac ggcaccagag agagtttgaa gccgaggccg acaatcatca ggccgaaacc
     1501 tgccagcaac agcggctcgt tgagcatacc gtcgccgagg tttttaccca gcgcgacaaa
     1561 cgacaggtcg ccagactgcg catacaccag cgccataccg aacagcagga aagaagacgc
     1621 tgcggcagag agaatggtgt atttgatact ggcttccagc gaacgtttct ggcggaaagc
     1681 gtaaccgacc aggccaaaca gcggcaaaga gatcagttcg ataccgagga acagagacgc
     1741 cagatggttg gcattcgcca gcaggatccc gcccagggcg gcaattaaca ccaacaggta
     1801 gaactcgtcc ttgttgtcgt tatagccttc aagccacggg taggcgaaag tacaggtggc
     1861 gaggctcgcc aacaatacca gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag
     1921 tggcgtaacg tccatagcgc ccgcctggcc gacaaaccag agcgaaacca gcgccgcgtt
     1981 aagcccaata accgagagcg tagcgttgag gaaatgattg cgtcgccacg caatggagag
     2041 catcaccacc accaccgtca agccgacgat cagcaacggt agcagtgcga tcaggttttg
     2101 tggagttatt gtcatggcga tttacggcct tgtagtagta acggaattaa caaaccactg
     2161 ctggatattg ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat agaagcccag
     2221 cagtaccagc agcaccacca gcaacaggat cataaacagc tcacgcagcg acatccctgg
     2281 cagttcctgg ctggcaatct ggcttttcgc tttaccgaag taagcgcgat gtaacatcgc
     2341 cagcgaataa acagatgcaa agaccagccc aaaggtagag ataacggtaa tcaccgggac
     2401 aacctggaag ctgccgaaca gaatcataaa ttcgccgacg aagttaccgg tgccaggcat
     2461 cccaagcgtt gccaccgcaa agaacagcga cagtgctggc agccatttca tcttgctcca
     2521 cagaccgccc atcatgcgca tgtcgcgggt atggatacgt tcataaagct gaccgcagag
     2581 aataaacaga cccgccgccg acaagccgtg cgcaatcatc tggattaccg cgccctggta
     2641 ggccaactgg ctgccagtgt agatagcaat cagcacgaag cccatgtggg aaaccgaggt
     2701 gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc atccacgcac cgtagaagat
     2761 gccgataaca cccagccaca tagcgattgg cgcgaactct gccgacgcgt tcgggaacaa
     2821 cggtagggag aaacgcagca gaccgtaagc ggcggttttc agcaagatcc ccgcgaggtc
     2881 aacggaaccg gcggttggag cctgtgagtg cgcatccggc agccagccat gcagcggaac
     2941 caccggcatt ttcaccgcaa atgcgatgaa gaagcccagc atcaacagat attccacacc
     3001 actggacatt ggcgtattca acagctcttc atagttgaag gtccagacgc cggtcgcatt
     3061 gtagtgaaca aaaaccagcg ccaggatggc gatcaacatc accagaccac tcgcctgggt
     3121 gtaaatgaag aacttggttg ccgccgtgat acgcgtttta ccgtcagagg ctttatgccc
     3181 ccacagtgcg atcaggaagt acatcggcac cagcatcatt tcccagaaga agaagaacag
     3241 gaacatgtcg atggcaagga acacgccgat aacgccgccc aggatccaca tcaggttgag
     3301 gtggaagaag ccctgatatt tttcgatctc tttccacgaa cagagtaccg ccagcacacc
     3361 gagcagaccg gtcagcacga ccatcagcag cgacagcccg tcaatggcga gatggataga
     3421 gataccaaaa cgcgggatcc acggcatgtc gaattcagac tgccactgcg gaattccggc
     3481 ggattgcgtc agtgaataac cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa
     3541 tcccatggtg atcagcgcga tccagcgcgg caccttgacg ccaaagcgtt cggtctgcca
     3601 gcacaggaag ccgccgataa agggaattaa tattagccag ggtagtaaca tggcgatctt
     3661 tattccttgt aaaagtcccg tcaggaccgg attttcaacg aattctcacg acagaaaatt
     3721 ctcttcggac tgggggtagt gtcggatgca ccgcgaatgc ggcttatccg acctacgttc
     3781 ttatatgccc aggggcaatc ccacaatcct taactcaacg cagtaccatc aacagtgcca
     3841 gcaccacgac cgcaccgatg ctcatggatg ccacatacca gcgcagataa ccgttctcgc
     3901 ttaacagcag acctttacct gcaaagcggg aaaggacagc cgggatgttc atcattgagt
     3961 tcagcggatc gcgtttcagc aaccaggcaa tacccaggaa cggcttgacg aacactttgt
     4021 cgtacagcca gtcaaagccc caggcgttgt accaccaggt acccagcaga cggcccggcg
     4081 cactgttggc gatggaggtc accagagtac gtttacccag ccacagccag gcagccagca
     4141 gaatgccgac caccgcgacc acgccagagg taatttccag ggtcaacatg ctgccgtgcg
     4201 ccagttccgt cgtttgcgga agcacgccct gcagcggcgg tacaatcagt gcgccaacga
     4261 aggtggaaag gatcagcagc acaatcagcg gcaggctgtg agttacccct ttcacggcgt
     4321 gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat catacggaag gtgtagagcg
     4381 aggtcataaa cgcaccgacc agacctgcca ccatcagatt gatatgacca ttcgccatcg
     4441 cacccgcgag gatctcatcc ttactgaaga agcccgcagt gaccagcggc agtgccgaca
     4501 gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg aatagattta cgcagaccgc
     4561 ccatcttgaa gatgttctgt tcgtgatggc aggccagaat gacggaaccg gatgccagga
     4621 acagcagcgc tttaaagaac gcgtgggtca tcaagtggaa aatcgccgca tcccatgcct
     4681 gcacgccaag cgcgaggaac atgtagccaa tctggctcat ggtagagtaa gcgagaacac
     4741 gtttgatatc ggtctgtacc agcgcggcaa aaccggccag cagcagcgta accgccccga
     4801 caatgcccac cagatgcaga acttccggcg tcatcaggaa caggccgtgg gtacgagcga
     4861 tcaggtagac acccgcggtt accatcgttg cggcgtggat cagcgcggag acaggcgtcg
     4921 ggcccgccat cgcgtcggca agccatgtct gcaacggcaa ctgcgcagat ttaccgaccg
     4981 caccgcccag cagcatcagc gtcgcccaca tcagcatgtt attgccgtca gcaaagtgcg
     5041 ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg taaagaatga
     5101 acagcgcgaa agcgaggaac acgtcaccca cacgggtcac gacgaacgct ttcattgccg
     5161 ctgcgccatt cttcggatcg gtgtaataga aaccgatcag cagataggag cacaggccca
     5221 cgccttccca gccgaggtac atcaacagta ggttatcggc gagaaccaga actaccatgc
     5281 tggcgatgaa caggttggtg taagcgaaga agcgagagta gccctcttct ccgcgcatat
     5341 accaggaggc atacatgtga ataaggaaac ccacaccagt gaccactgag agcatggtca
     5401 gcgacaggcc gtccagcacc agattgaaac caatgttaaa gtcgcctacc gacatccacg
     5461 tccacagcgg ctggctgtat gcctgctcac cgttagcgaa gaagtcaacg ccgataaagg
     5521 cggttaccag cgccgccagg cccacagagc caacgccgac gatcgccgag acgttttcag
     5581 accagcgccc acgggagaac gccagcagga cgaagccaat caatggcaaa ataatggtta
     5641 aggcaagcat gttcatccgc gcatctcact tactgaatcg atgttcaggt tctggcgacg
     5701 acggtgaagt tgcagcagca gcgcaaggcc gatactcgct tccgccgccg cgaggctgat
     5761 ggcgagaatg tacatcacct gaccgtcggt ctggccccag tagcttccgg cgaccacaaa
     5821 ggccagcgca gaggcgttaa tcatgatttc cagaccaatc agcataaaca gcagattgcg
     5881 acggataacc agaccggtta agccaagaac gaataagatt gccgcgagga tcagtccatg
     5941 ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc tttacgattg
     6001 ctcagcactt cacccgcacg ctcttcacga ccgacgtgga aggccacaac cagacccgcg
     6061 agcagcagca tagaagccag ttccaccgcc agtacgtaag gcccgaacag cgtaataccc
     6121 actgctttag cactgattgg cgtaccgtcg ataccctgat cattaacacc aaggatggcg
     6181 taaacaatca ccaccagcat gatggccgac aaaatcgccg gaccaatcca cacctgcggt
     6241 ttcagccact ggcgttcctg ttcgatttct gaaccgccca ggttgagcat catcaccacg
     6301 aacacgaaca gcaccataat ggcacccgcg tagacgataa tttccagcgc accggcgaag
     6361 taagcgccca gtgagaagaa caccccagaa atcgccagca gcgaaataat caggtacagc
     6421 agcgcgtgta ccggattggt atgggtgatc actcgcaagg tcgcaagtat ggctatcagg
     6481 ccacagatat aaaaagcgaa ctccattgcc cctctcctta cggtaacagg ctcttgacgt
     6541 cgataggctt ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt gccatacctg
     6601 ccatccggta gaagttatat tccgggtatt tgcccggacc ggagatcagc agatcctctt
     6661 tctcgtaaac cagatcctgg cgcttgtatt cccccatttc gaaatccggg gttaactgaa
     6721 tcgccgtggt cggacaggct tcttcgcaca gaccacagaa aatgcagcgt gagaagttga
     6781 tgcggaaaaa ttccggatac cagcgaccgt ctttggtttc tgctttttgc agcgagatac
     6841 agccgaccgg gcaggctacc gcgcagaggt tacaggctac gcaacgctct tcgccgtccg
     6901 gatcgcgggt cagaacgata cgaccacgat aacggggcgg cagatagacc ggctcttccg
     6961 ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag atactacgaa
     7021 cctgggtgcc gaaacctact aacaattctt ttaaggtcat ggtcttattg ccccttattg
     7081 cgcctgccag agaatgacag ccgccgttac cagcaagttg atcagcgtca gcggcaggca
     7141 gattttccag ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg acgcacgaat
     7201 caaaatgaac atcatcataa agaacgcggt tttcagcgcg aaccagatga atggcggtaa
     7261 caacgggcct tgccagccac cgaagaacag cgtcaccatc agtgcagaga tggtcacaat
     7321 cccgatgtat tcgcccacga agaacagacc gaacttcatg ccggaatatt caatgtggta
     7381 accatccgcc agttcctgct cggcttccgg ctggtcaaac gggtgacggt gacacaccgc
     7441 cacgcccgcg atggcaaagg taataaaacc aaagaattgc gggatgacgt tccacacatg
     7501 cgcctggctg ttgacgatgt cggtcatgtt gaatgaaccg gcctgcgcca ccacgcccat
     7561 cagggaaagc ccgaggaaca cttcgtagct cagggtctgc gcagaagcac gcatcgcacc
     7621 cagcagcgag tatttgttgt tacttgacca gcccgcaaac agcaccgcgt aaaccgccag
     7681 accggccatc atcaggaaga acaaaatccc gatgttcagg tcggcaacca cccaacccgg
     7741 actgactggc acaatcgcaa aggccagcag cagcgaggta aaggcaatca tcggtgccag
     7801 ggtaaagatg acgcgatccg agaatttcgg gatccagtct tctttaaaga acatcttgat
     7861 catgtccgcg accagctgga gcgaaccgcc ccagccaaca cggttaggtc cgtaacggtt
     7921 ctggaacaga cccagcaggc gacgttcgcc aaagctcatg aatgccccac aggtgacaac
     7981 caccagcagg atcaccaccg ctttgaggat ggtcagcagg atctcaatca gttccggtga
     8041 tatccaactc attgttgtgc ctccttgaga tcctcaagat gcgcgccagc cagcaccgga
     8101 gcaatgccgg acatacccat cggcaagccc acctgccctg ccgtcagtcc ttcggcgatt
     8161 tcaaccggca gcgtgaccgt gttgccatcg taactaaagg agacgcgtgt acccgcgttc
     8221 acacccaact tcgcggcatc cgctgggttg agtttgatgt acggctgcgg catacggctc
     8281 tggaagaccg gagcacgctg tgacaattca tcgctaccaa acaggtggta gtacggcgcg
     8341 atacgccatt tcccgtcctg cggctggaag cgtgccggta cgctggtgaa gtaatccaga
     8401 ccattttcgc tggtttcaaa cagacgcacg cccggatcgc caaagcgcag tttgccgccc
     8461 acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc agcctggcgc ccaggcaaac
     8521 ggcacttgcg aacggtgcgc ggtcggctgg ttgttacctt ccatcgagaa ggtgaacatg
     8581 gtgtcaatat cctgcggctg acgcggctca tgaacgctga tattggcgcg catggcggta
     8641 cgaccgctgt aacggtgcgg ttcacgggcc agtttctgcc cacgaatacg gaatgtcgca
     8701 tccggcgcag catctttgat acccgccagt tccgggattt tcgccacaac agcgtcaatc
     8761 acatggtcga gctgcgtcca gtccacttca cggctcagca aggtgctgtg cagcgagtgt
     8821 aaccaacgcc agctttccag catgacagtt ttgctgtcgt aataagccgg atcgtaaacc
     8881 tggaagaaac gttgggcacg gccttcgttg ttaatcaccg taccgtcgct ttcagcaaag
     8941 ctggcagcag aaagtaccag atgggcgttt tccataatcg ctgtgcgttg atggtcaacc
     9001 accatcacca gcggcgcttt agccagcgca gcattcacgc gggtagcgga agcgtgacga
     9061 tgcagatcgt tttccagcac caccaccgcg tcggcgcgtc cggtttccag ttcggttaac
     9121 gcttcttcaa gcgatccacc gcccataatg cccagcccca tgctgttgac ggaacgggca
     9181 atcatggtga taccgacgtc agcgccgcga cctttcaggg ctttcgccac gttagccgca
     9241 gcctgaatca cttcagcgct accggcgttc gtcccggaga taatcaacgg tttcttcgca
     9301 cctgccagtg cctgcacgat gacgtcgatt ttgctttgca gctcaggttc aataccgtca
     9361 accgctggcg cggagttatc cagcgcatgg gcgatggcaa aacctaaacg cgcctgatct
     9421 tcaaccggtg cgcggtaagt ccacgccgcg atatcatcca gacgggtgtc atcaacgttg
     9481 gtaacaaaca gcggatgctt cgcacgttgg ccgatgttga ggattgccgc aatctgccag
     9541 tcagccactt tctgtgctgc cgccatttcg cgcgctttac ctttcaccgc ctgacgaact
     9601 gccagcgcga cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag taccgcatcg
     9661 taagattcga tttcgcgcag tgccggcgta taaatgccgc cttcacgcag cactttcagc
     9721 gccagttgca gacgttcctg ctcaccgtga gcgataccgg tgtagaagtt ttcttcgccc
     9781 accagctcac gcagcgcaaa gttgctttcc acgctggcac gcggagaacc aatgccgatc
     9841 actttcttcg actgacgcag aatatctgcc gcgccctgca ttgcctgttc ggcgttgagg
     9901 gtaatgaaat catcgccacg acgctgtact ggctgacgcg gacgatcttt caggttgacg
     9961 taaccgtaac cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt accgttgtaa
    10021 cggttttcga tacgacgcag ttcgccgtaa cgctcgccgg ggctgatgtt acagccgatg
    10081 gaacattgct ggcagatgct cggcgcaaac tgcatatccc atttacggtt gtaacgctcg
    10141 gagtgcgttt tgtcggtgaa tacgccggtc gggcaaatct cgaccagatt accggaaaat
    10201 tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcgtg cgcaccgtaa
    10261 acgcccaggt ccgtgccgtc agcgtaatct ttgtagtaac gcacgcagcg gtagcaggcg
    10321 atgcagcggt tcatttcgtg agagatgaat ggccccaaat cctgattacg gtgggtacgt
    10381 ttggtgaaac ggtagcgacg gaagctgtgt ccagtcatca cagtcatatc ctgaagatgg
    10441 cagttaccgc cctcttcaca taccggacag tcgtgcgggt ggttggtcat taaccactcg
    10501 accacacttt cacggaactg tttcgcttct tcatcgtcaa tggaaataaa ggtgccatcg
    10561 gaagccggtg tcatacagga catcaccagg cgaccacgcg tgtcttccgc gttttggtat
    10621 tgcttcaccg cacactggcg gcaagcaccg acgcttccca gcgccggatg ccagcaaaag
    10681 taaggaatat caaggcccag agacagacaa gcttccagca agttgtccgc tccgttgacc
    10741 tcgtattctt tgccgtctac atgaattgta gccattagca tgcttccagt tttctcagtc
    10801 agagactgag cgttaatcga aattcggtta ccagcgctct ttcagcaggt tcggctgaat
    10861 cccattaatc aaatgggtat tgctgaacgg ctgtttgatt cccgcctcaa attcttcgcg
    10921 gaaatatttg atggcgctct gtaacggctc cactgcacca ggtgcgtggg cacagaaagt
    10981 tttacccggg cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc ccggctgacc
    11041 ttcgccacgc tccagcgcac gcagaatttt cacgctccac ggcagaccgt cgcggcacgg
    11101 cgtacaccag ccacaggact cacgggcgaa aaactcttcc aggttacgca ccagcgacac
    11161 catgttgatc tcatggtcaa ccgccatcgc cagcgccgta cccagacggc ttcccgcttt
    11221 accgatactt tcgaattcca tcggcagatc aaggtgcgct tcggtcagga agtcggtgcc
    11281 agcgccgcct ggctgccagg ctttgaattt cagaccgtca cgcataccac cggcgtaatc
    11341 ttcgaggatc tcgcgtgcgg tggtgccgaa tggcagttcc cacaggcccg gatttttcac
    11401 ccgaccggag aagcccatca gcttggtgcc agcatcttta cttttcgaga tgttctgata
    11461 ccactccacg ccgttagcga ggatcgccgg aacgttacac agggtttcga cgttattgac
    11521 acaggtcggt ttgccccatg caccggaggt tgccgggaat ggaggcttcg agcgagggtt
    11581 agcgcgacgc ccttccaggg agttgattaa cgctgtttct tcaccgcaga tgtagcgccc
    11641 agccccggta tggacgaaca attcgaagtc gaaacctgtt cccataatgt ttttgccaag
    11701 cagacccact tcggtggctt cggcaatggc acggcgcaga ttcactgccg cttcgatata
    11761 ctcgccacgc aggaagatgt agccacggta cgctttcagc gcaaacgcgg agatgagcat
    11821 accttccacc agcaggtgcg gcagttgttc catcagcagg cggtctttat aggtgcccgg
    11881 ctccatttca tcggcgttac acagcaggta acggatgttc atggattcgt ctttcggcat
    11941 caggctccat ttcaggccgg tagaaaagcc cgcgccgccg cgccctttca gaccagcgtc
    12001 ttttacctga ttaacgattt cgtccggaga cagcccggtc agcgccttac gcgcgccttc
    12061 gtaaccgttt ttgctgcgat attcgtccag ccacactggc tgtttgtcat cgcgcagacg
    12121 ccaggtcagc ggatgcgttt cgggagtacg gataatgttt ttcatttata ccgctccagc
    12181 agttcaggga tcgcttccgg ggtcagatgc gcgtgagtgt cctcatcgat catcatgttt
    12241 ggccctttgt cgcagttccc caggcagcaa gttggcagca gcgtaaagcg gccatcaaat
    12301 gtcgtttgcc ctggtttgat gttcagcttt ttctccagcg ccgcctgaat accctggtaa
    12361 ccgttgatgt gacagaccac gctgtcgcaa taacggatca catggcgacc aaccggctgg
    12421 cggaagatct ggctatagaa cgttgccacg ccttcgacgt cgctcgccgg aatacccagc
    12481 acatcggcga tcgcgtggat cgcgccatcc ggcacccagc cacgctgctt ctgaacgatt
    12541 ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc gtgctcaatc
    12601 gcttcacgct ctgccgcact cagctcaaaa gcctcggttt gtggttgttg attctcgtgc
    12661 ataattagcg gtccacatct gacataacaa aatcgatact gcccagataa acaatcaggt
    12721 cagacaccag gctgccgcgg atcgccgccg gaatttgctg caaatgcgca taactcggcg
    12781 tacggatacg ggtgcgataa ctcatggtgc taccgtcgct ggtcaggtag taactgttga
    12841 tccccttggt cgcctcaatc atctggaaag attcattggc tggcatcacc ggaccccacg
    12901 acacttgcag gaagtgggtg atcagggttt cgatatgttg cagcgtgcgc tctttcggcg
    12961 gcggcgtggt cagcggatga tccgctttga acgggccttc cggcatgttg ttgaggcact
    13021 gctcaagaat gcgcagactc tggcgcagct cttccacttt cagcattacg cgggtgtagc
    13081 agtcagaaac accaccaccc accgggattt caaagtcgaa gttttcatag ccagaataag
    13141 gacgcgcctt acgcacgtcg aagtcgatcc cggtggcgcg caggcccgcg ccagtggtgc
    13201 cccactccag cgcctctttc gcgccatagg cggcaacgcc ctgggaacga cctttcagaa
    13261 tggtgttttg cagcgccgct ttctcgtaag acgccagacg tttcggcatc cagtcgagga
    13321 actcacgcag caggcgatcc cagccacgcg gcaggtcgtg cgctacaccg ccaatacgga
    13381 accacgccgg gtgcatacga aaacccgtga tcgcttccac cagatcgtaa attttctgac
    13441 gatcggtaaa ggcgaagaac actggcgtca tcgcgccgac gtcctgaata aaggtggaga
    13501 tatacagcag gtgactgttg atgcggaaca gttcggagag cattacgcga atgacgttaa
    13561 cgcgatccgg cacggtgatc ccggccagtt tctctaccgc cagcacgtaa ggcatttcgt
    13621 taacgcagcc gccgaggtat tcgatacggt cggtatacgg aatgtagctg tgccaggact
    13681 ggcgttcgcc cattttctcc gcaccacggt ggtggtagcc gatgtctggt acgcagtcga
    13741 caatttcttc gccatcaagc tgcaaaacga tgcggaaagc cccgtgcgcc gacgggtggt
    13801 tcgggccgag gttgaggaac atgaagtcct cgttttcggt gccgcgcttc atcccccact
    13861 cttccggttt gaaggtcagg gcttccatct ccaggtcctg tttggctttg gtcagctcaa
    13921 acggcgagaa ttcggtagcg cgcgccggat aatctttacg cagcgggtga cctttccagg
    13981 tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc gaaagtaatg ccaaacagat
    14041 cccaggtttc acgctcatac cagttagcgt tcgggaacag tttggtgaag gtcggtacgt
    14101 gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc gcggttacga tcgatagaaa
    14161 tcagatggta gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgt gtgcgcagac
    14221 gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt ttctttaaga
    14281 aatcgcccac ttccagtaat tgttcacgct tgatccacac aacgggaacc ccggtgcgag
    14341 tcgcctgaac agtaaaggca tccggcccaa aacggttgcg cagttcgcca atcaccggat
    14401 catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc ggttaagtcg gtcatattgt
    14461 tcaccattgc aaatggtccg tggtgactgt cggcgcagta agcttcgcga aatagattta
    14521 ggaataagcg aagaaaaatt cctttgccga caggcgcaaa ttaaatctcg tcaggtgtac
    14581 gcaggttggt tacggcaatg cgttcaccgc gcttgcgttc gcgctctgat tgcatattgg
    14641 cgcgataaac gccctgatcg ccaaccaccc aggagagcgg acgacgttct ttaccgatag
    14701 attcctgcaa cagcatcagt gcctgcatgt acgcttcagg acgcggaggg cagcccggga
    14761 tatacacatc aaccgggatg aatttatcga cgccctgcac aacggaataa atatcgtaca
    14821 taccgccgga gttggcacag gcacccattg agataaccca ttttggttcc agcatctggt
    14881 catacagacg ctgaataacc ggtgccattt ttgtaaagca ggttcctgca acgaccatca
    14941 ggtcagcctg acgcggcgaa gctcgcaata cttctgcgcc aaaacgcgcc acgtcatgca
    15001 ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga aagaccgaag ttatacggcc
    15061 aaattgagtt tttacgaccc cagttaacca tgtcattgag cttgcccata aacacgtttt
    15121 tattaacttc ttgctccaga gggtcggtta cgatctcctg cttttgcagg gggtaacggt
    15181 cgttctcacc gttgggatct atgcgggtga gcgtataatc catcttaatg cctcgcggtt
    15241 agcgttgacg attagcgata ctgttcgttt ccgggttcat acgctcgcgg cgtgaacgcg
    15301 cgggcgtcca gtccagcgcg ccaatacgca ccagataaac cagacctgcc agtaacacaa
    15361 aaataaaaat tgcagcttcc acaaagccta cccagccgct ttcgcggata gaggttgacc
    15421 atgcgaacag atacagcgct tcaacgtcga agataacgaa gaacatggcc accagataaa
    15481 acttggcgga caggcgtaag cgggcggagc cgaccgagtc gataccggat tcaaacggaa
    15541 cgtttttcga ccttgcgcgt gcgcgaccgc ctaaaaacca accgcctacc agcatcaggc
    15601 aacacaggcc aatggcaacg ataagaaaga tagcgaatgc ccagtgatga gcgatgactt
    15661 cagtggatgt tgacatactc attgcttact catcaaaagt agcgccagat tcactgctct
    15721 tcacggcaga tggacgccac atcgattcat ggggaggaat aaaaaaaacc ttacaatcac
    15781 tgtagaaatt cttttataca gctaattgat gtggtctttt actcctttct ataacctttt
    15841 gtcaacttta acaaaagttt cttcacatta gtttacataa tatcaacacc attagcattt
    15901 aatgcccttt caccccagat ccttgacgac tccaggataa ttagatgttg ttgaatcgtg
    15961 tccgttgtga agcaatggaa aaaatacggg tctattttga caggaatttg tgtcgattcc
    16021 tccccccaaa agagagtatt ttcttgatct gtgacacgct tttgtcattc cataacaaaa
    16081 acgcagcaac aaatttacgt attttttaac atcattgtag caggtgattt ttttcaggcg
    16141 attatttgtg cgttcgggac gtgaatctct ggtgattgaa aaatgaacag ttttgtacgt
    16201 tctgcacgat acgaggaagg ctttttacta aaaaaaagcc gctggggttt aaaacacccc
    16261 cagcggctcg tttttacact attgtctcag aaattatcta tggtccgtcg attactcgat
    16321 atccctttca atcaacaatg agtcatcgcc ttccggagca gacattgggc tgtattgcca
    16381 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
    16441 cgggtcatag cacagcaaat attcggtatc gggtagcggc ggtaagccat ctactccact
    16501 cagcacacgc aggtctggac tcatcatctc gaccggcctt gccgtcacgc caagaccggc
    16561 ttttactgcc gcacgaactg ccggaagcgt cgaggcgaca taagccagtc gccatggaat
    16621 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac gggctaggat catccagcaa
    16681 tacaagaggg atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
    16741 tggcgaggta cgcaggttca atgccttaaa cgttgaagga cgatgggtgg ttaccatcaa
    16801 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
    16861 cagcgctagt ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
    16921 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
    16981 tgagcaggcc tcatcattaa aacgcagaat ttttctggcg taaccaagaa gttgaatgcc
    17041 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
    17101 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
    17161 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
    17221 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
    17281 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
    17341 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
    17401 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag gaatcgcata
    17461 tctattaagt tactcactct tttgtattta tgacatgcgc gtgtttgtat aaatgtaaat
    17521 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
    17581 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
    17641 aaccttttat ttataagggt cattgcgaat tatctgatgc aaagttatgt tatgttaggc
    17701 aaagtaatct tctattatta ataagcacaa caaaaccttt ttgaatatta aataataatt
    17761 aattagcatc atcctcatac attaatcccg cttaacaata gtttcacacc ttgcacccat
    17821 tacgacacct cggaaatcat cacgctgtta aactctccgt aatgcggaaa tatcatcatc
    17881 acgattcatt agtgaattct taaacgaggc attttcaaac aattatctta cagataaaaa
    17941 aaccacagtc acaattaaga atcagaacaa ttcactacat aacattaatt actaagcata
    18001 tacacctcat tattttgcca ttattaagtt attaacagca caatcgagtc ttcccctctg
    18061 gcaaaatctt attctgcaga ccttcaaaac gccgtcctgg gggagtacat tgttctaagc
    18121 tgacttccac ggcagggagt ggcgataaca gcaaaaaagg tcaagattca tgtcccccat
    18181 tgaaaaatcc agcaaattag agaatgtctg ttacgacatc cgtggtccgg tgctgaaaga
    18241 agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg gcaaccctgc
    18301 cccgttcggt tttgacgcgc cagatgaaat cctcgttgac gtgattcgca acctgcctac
    18361 cgctcaaggg tattgcgatt ccaaagggct ttactccgcg cgtaaagcca tcatgcagca
    18421 ctaccaggct cgtggcatgc gtgatgttac cgtggaagat atttacatcg gaaatggtgt
    18481 atcggagctt atcgttcagg caatgcaggc attgctgaac agcggcgacg aaatgttggt
    18541 tcctgcacct gattacccac tatggaccgc ggcggtttcg ctctccagcg gtaaagcggt
    18601 gcattatctt tgcgatgaat cctctgactg gttcccggac cttgatgata ttcgcgctaa
    18661 aattacgcct cgtacgcgtg ggatcgttat tatcaaccca aataacccaa ccggcgcggt
    18721 atattccaaa gagcttttaa tggagatagt ggagattgca cgtcagcata atctcattat
    18781 cttcgccgat gaaatttatg acaaaattct ctacgacgac gctgagcatc actcaattgc
    18841 gccgctggca cctgatctgc tgaccattac ctttaacggc ctgtcgaaaa cataccgcgt
    18901 tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta
    18961 catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca
    19021 cgccattcag accgcgctgg gcggttatca gagcatcagt gaatttatta cccctggcgg
    19081 tcgtctttat gagcagcgta accgcgcatg ggaattgatc aatgatattc cgggcgtttc
    19141 ctgcgtgaaa cctcgtggtg cgctgtacat gttcccgaaa atcgacgcca aacgctttaa
    19201 tattcacgac gatcagaaaa tggtgctgga tttcctgttg caggaaaaag ttctgttggt
    19261 gcaagggacg gcattcaact ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg
    19321 tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca
    19381 gctgtaatct taatttcact gccggagatt gcatccggca gtgttatccc gccacaatga
    19441 cctgatgatg tcatcatacg taaggtcact atgaaacaga gccatttttt tgcccatctc
    19501 tcccgcctga aactcattaa ccgctggccg ctaatgcgca atgtgcggac ggaaaatgtg
    19561 tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc tggcagctat caaaaatcgt
    19621 aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta ccacgatgcc
    19681 tcagaagtgc ttaccggcga tctccctacc ccggtgaaat acttcaattc gcaaatcgct
    19741 caggaataca aggcgattga aaaaatcgct cagcaaaaac tggtcgatat ggttccggaa
    19801 gagttgcggg atatctttgc gccgttaatt gacgagcatg catatagtga tgaagaaaaa
    19861 tcactggtga aacaggcaga tgcactatgt gcatatctga aatgtctgga agaactcgcg
    19921 gcgggaaata atgaattctt gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgc
    19981 cgcagccagg agatggacta cttcatggaa gtctttgttc ccagcttcca tctttcgctc
    20041 gatgagatta gccaggattc accgctgtaa gcagccggag tctgcgtcgc atcaggcaat
    20101 aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg atcatcacca
    20161 cacaaaccgc catcacgata atggtgaacg gtacccccaa cttcacaaag tcactgaagc
    20221 tgtaatttcc cggacctaaa accagtgtgt taacaggtga agaaaccggc gtcataaagg
    20281 cggcggatgc tgccatcgcc acgaccatcg cgaatgggta aggcgacacc cccatcgttt
    20341 tagcagcagc cagcgcaatc ggagccatca acaccgccgt cgcggtatta gaaataaata
    20401 gcccaataac cgccgacaag acaaacaaac aacccagcat catatgtggc ccgtaaccgc
    20461 cgccaatgtc catcagccct ttcaccgcca gcgcaacacc tcccgttttc tgtaatgcca
    20521 cagcaaacgg catcatccca acgatcaaaa taatgctcgg ccagtgtatg gatttatagg
    20581 cgctttcagc atcgatacag cggaatttcc ccatcagcag gcaggcgatg atagcggcaa
    20641 caggattagg aatttcatcg gtcagcatta acgccaccat tagcaccaga cagaaaatgg
    20701 catggggtgc ctgactgtgc gcgggcgatg cttcactcac ctcttccggt aagttcagcg
    20761 ctacgaagtc gcggccctgt ttggccagca taccgatcag tttccagtta cccacaacca
    20821 ggatgatatc gcccagcagc agaggctcat ccgccagcga accttccagc gccacgccat
    20881 tgcgctttag ccccactaca ttcaatccgt agcgggtacg aaaaccaatt tcgcgcaccg
    20941 atttaccaat aagttctgac tcaggaatta atgaaatctc tgccatgccc acatcaaggg
    21001 cctggtcaga aaaatactcg ccgcgcagta ccatcggctc cagcaattgc tcactacaaa
    21061 attgccggag atcgacatca gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa
    21121 attcagaaac cccattaacg ttcacgataa cgcgacgaaa acgccgccag cgttcaacac
    21181 cgatgacgtt agcaccataa cgttcacgta atttgagatc gtccagtcgt tgaccaatca
    21241 ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga tattcacgga
    21301 taagatcgcg aaaggttcgg cgcgtccagc cttcgcgttg tggggtctgg gtatcccctt
    21361 tcagcatgaa acgcatcact aacatataca aaatacccag caccagcacg accaggccaa
    21421 taggtgttac gctaaagaaa ctgaagccgt gatagccttc acgcagcaat tcactgttga
    21481 ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc gcttatcagc ccggcaaaac
    21541 ttaacggcat catcagacgc gacggcgacg tttgcatacg catggcaacg cttaacacca
    21601 cgggaataaa gatagcgaca acgccggttg aactcataaa cgcgccaagc cccgcgacgg
    21661 tcagcatcaa caaaaccaac attttgattt cactgttgcc cgccactttg accagccatg
    21721 tccccattac agtggcaaca ccggtacgga ccaaaccatc gccaataatg aacaaggcgg
    21781 caatcaggac aacgttagga tcggaaaagc cggaaaatac ttcggggacc gtcagcgttc
    21841 cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc atccatacgc actctgcccg
    21901 tcgcaaacaa gacgatggca acggccagta atgaaagaac ccaaatcaat tcaccgttca
    21961 caacttatcc ttgttaattg agggggataa cttgattctg ccataaaaaa gccccgacga
    22021 gacggggcta aatcatgatc aggtgtttca ctgaataata acatcgccat ttggctgttt
    22081 ggtcacagta atttgctcca gactgtggag gaccaaatcg acctcattca ggcgcggggt
    22141 atctgccgga gcgttaaccg caatgacatg gcaacccgcc gccaggccag aaagcacgcc
    22201 agcgggagca tcttccacca ctacacactc ctgcggcgca agccccagca gctgcgcgcc
    22261 tagcagatac gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg tcacaaacac
    22321 ctctggcgcg ggaagcccag ctattttatg gcgcgctcgc gctaccggca tggagccaga
    22381 agtcacaatg gcccacggaa tacctgcttt attcaaatga ttgagtaagg cgattgcccc
    22441 cggaagtgcg gtaataccct cggtttccgt ggcctcgatt tgctccagac gcgtaaactc
    22501 ggcggcaata tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg tgatcgcctg
    22561 tttaccgtga ataaaagcca gcacctcttc cggcgctaac ccatgacgtc tggcccagtt
    22621 gctccacgcc cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag
    22681 aaaacctttg caccgcacgc gggcctcctc aggcgttgat aatttgatta atttcgttgg
    22741 cgctcaaatg gtactgacgc gggcaggcat gccacacatt aagcatgcgc tgatattttt
    22801 cccacattgg cgtctgggcg ttaaaaccgt gagttccggc atcaaaatgg gtatagcgtc
    22861 cttccacatt aaccataaag cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa
    22921 agccgaggaa ggtgacacga cgttcatcga tggattgctg gtcctgcaaa ttagaccagg
    22981 aaacatgcaa cgcatgatac atctccataa tgtcgatgat agtgcggcag gtttcttctt
    23041 tcagctcgcc aaactcgcga tccagttcac gcatctgtaa tccataacca cgctcaatga
    23101 ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc gagcatagtc atcatcttgt
    23161 actggttaga caaaataaga cgttgcgcgt tggtcatttc cattgttgac tcctgtatca
    23221 ctctactacg gtgaaaaaaa aagaaggctg agtatgcctt cttttatatg cgtaatcagg
    23281 ggtcaattac aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc gcttaagcgt
    23341 gtcagctaat gcctggtaat caggcttgcc ttcaacgggt gccaacacct gtccagactc
    23401 ctgcaattta ccgcgaactt cataaaacca gttaaggatt gcagggggta atggcgttac
    23461 agaacgtttg cccagccacc acaatccctg catgggtaaa cttaaggcga acagcgcagt
    23521 ggcaactgcc ggcccaagct gaccgcccag ggcaatctgc cagcagagag taaatacggc
    23581 gatcggcggc ataaaacgga tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa
    23641 gaccggggca aggcgttttt ccagcggcca cgtctttgag taatgctgtc cccggcgaaa
    23701 caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc tcaacttcac
    23761 atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt tcaaaacatt
    23821 ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa gtagtacata
    23881 ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac gcaaaatggc
    23941 atagactcaa gatatttctt ccatcatgca aaaaaaattt gcagtgcatg atgttaatca
    24001 taaatgtcgg tgtcatcatg cgctacgctc tatggctccc tgacgttttt ttagccacgt
    24061 atcaattata ggtacttcca tgtcgagtaa gttagtactg gttctgaact gcggtagttc
    24121 ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc
    24181 cgaatgtttc cacctgcccg aagcacgtat caaatggaaa atggacggca ataaacagga
    24241 agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg ttaatactat
    24301 tctggcacaa aaaccagaac tgtctgcgca gctgactgct atcggtcacc gtatcgtaca
    24361 cggcggcgaa aagtatacca gctccgtagt gatcgatgag tctgttattc agggtatcaa
    24421 agatgcagct tcttttgcac cgctgcacaa cccggctcac ctgatcggta tcgaagaagc
    24481 tctgaaatct ttcccacagc tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca
    24541 ccagactatg ccggaagagt cttacctcta cgccctgccg tacaacctgt acaaagagca
    24601 cggcatccgt cgttacggcg cgcacggcac cagccacttc tatgtaaccc aggaagcggc
    24661 aaaaatgctg aacaaaccgg tagaagaact gaacatcatc acctgccacc tgggcaacgg
    24721 tggttccgtt tctgctatcc gcaacggtaa atgcgttgac acctctatgg gcctgacccc
    24781 gctggaaggt ctggtcatgg gtacccgttc tggtgatatc gatccggcga tcatcttcca
    24841 cctgcacgac accctgggca tgagcgttga cgcaatcaac aaactgctga ccaaagagtc
    24901 tggcctgctg ggtctgaccg aagtgaccag cgactgccgc tatgttgaag acaactacgc
    24961 gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg cgaaatacat
    25021 cggtgcctac actgcgctga tggatggtcg tctggacgct gttgtattca ccggtggtat
    25081 cggtgaaaat gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg tgctgggctt
    25141 tgaagttgat catgaacgca acctggctgc acgtttcggc aaatctggtt tcatcaacaa
    25201 agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa gaactggtta tcgcgcaaga
    25261 cgcgagccgc ctgactgcct gatttcacac cgccagctca gctggcggtg ctgttttgta
    25321 acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt cccgtattat tatgctgatc
    25381 cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg tgcaatggaa
    25441 cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac cggtggcgat
    25501 gcgcccgatc agactacgac tatcgtgcgt gcgaactctt ccaccacgac ggccgctgaa
    25561 ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca atcagaaaga tgtgctgatg
    25621 gaagagatcg tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt tctggttgaa
    25681 ggtctggtcc cgacacgtaa gcaccagttt gcccagtctc tgaactacga aatcgctaaa
    25741 acgctgaatg cggaaatcgt cttcgttatg tctcagggca ctgacacccc ggaacagctg
    25801 aaagagcgta ttgaactgac ccgcaacagc ttcggcggtg ccaaaaacac caacatcacc
    25861 ggcgttatcg ttaacaaact gaatgcaccg gttgatgaac agggtcgtac tcgcccggat
    25921 ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa acaatgttga tccggcgaag
    25981 ctgcaagaat ccagcccgct gccggttctc ggcgcggtgc cgtggagctt tgacctgatc
    26041 gcgactcgtg cgatcgatat ggctcgccac ctgaatgcaa ccatcatcaa cgaaggcgac
    26101 atcaatactc gccgcgttaa atccgtcact ttctgcgcac gcagcattcc acacatgctg
    26161 gagcacttcc gtgccggttc tctgctggtg acttccgcag accgtcctga cgtgctggtt
    26221 gccgcgtgcc tggctgccat gaacggcgta gaaatcggtg ccctgctgct gactggcggc
    26281 tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac cggcctgccg
    26341 gtatttatgg tgaacaccaa cacctggcag acttctctga gcctgcagag cttcaacctg
    26401 gaagttccgg ttgacgatca cgagcgtatc gagaaagttc aggaatacgt tgctaactac
    26461 atcaacgctg actggatcga ttctctgact gccacttctg agcgcagccg tcgtctgtct
    26521 ccgccagcgt tccgttatca gctgactgaa cttgcgcgca aagcgggcaa acgtatcgtt
    26581 ctgccggaag gtgacgaacc gcgtaccgtt aaggcagccg ctatctgtgc tgaacgtggt
    26641 atcgcaactt gcgtactgct gggtaatccg gcagagatca accgtgttgc agcgtctcag
    26701 ggtgtagaac tgggtgcagg cattgaaatc gttgatccag aagtggttcg cgaaagctat
    26761 gttggtcgtc tggtcgagct gcgtaagaac aaaggcatga ccgaaaccgt tgcccgcgaa
    26821 cagctggaag ataacgtggt tctcggtacg ctgatgctgg aacaggatga agttgatggt
    26881 ctggtttccg gtgctgttca cactaccgca aacaccatcc gtccgccgct gcagctgatc
    26941 aaaactgcac cgggcagctc cctggtatct tccgtgttct tcatgctgct gccggaacag
    27001 gtttacgttt acggtgactg tgcgatcaac ccggatccga ccgcagaaca gctggcagaa
    27061 atcgcgattc agtccgcaga ttccgctgcg gccttcggta tcgaaccgcg cgttgctatg
    27121 ctctcctact ccaccgggac ttctggtgct ggtagcgacg tagaaaaagt tcgcgaagca
    27181 actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct gcagtacgac
    27241 gctgcggtaa tggctgacgt tgcgaaatcc aaagcaccga actctccggt tgcaggtcgc
    27301 gctaccgtgt tcatcttccc ggatctgaac accggtaaca ccacctacaa agcggtacag
    27361 cgttctgctg acctgatctc tatcggaccg atgctgcagg gtatgcgcaa gccggttaac
    27421 gacctgtccc gtggcgcact ggttgacgat atcgtctaca ccatcgcgct gactgcgatt
    27481 cagtctgcac agcagcagta atctcgtcat catccgcagc tttacgctgc ggatatctga
    27541 accggaaata gtcactattt ccggtttttt attctcttaa tttgcattaa tcctttctga
    27601 ttatcttgct taactgcgct gcatcaatga attgcgccat ctcaatttgc atacttacca
    27661 ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc taaaccaaca
    27721 agaagatggg caatgcccga tacgttggtg attatctttt ttgttgccat tttaaccagc
    27781 cttgccacct gggtagttcc ggtgggcatg tttgacagtc aggaagtgca gtatcaggtt
    27841 gatggtcaaa caaaaacacg caaagtcgta gatccacact catttcgcat tctgactaac
    27901 gaagcaggcg aacctgagta tcaccgcgta cagctgttca cgacgggcga tgaacgcccg
    27961 ggtctgatga acttcccgtt tgaagggtta acctcaggat cgaaatacgg gacagccgtt
    28021 ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat gcgtacagga
    28081 accattgata acggtatcct ggcgcttatt cgccataccc gcggaaatga aattctcttt
    28141 attcctgcgc tgtttattct gttttcactt ggcggcgcaa tatttggtat gggagaagag
    28201 gccgtcgcct ttgccattat tatcgcaccg ctaatggtcc ggctgggcta tgacagtatt
    28261 accaccgtcc tggtgaccta tatcgccacg caaatcggtt ttgccagttc gtggatgaac
    28321 ccgttttgtg tggttgttgc tcaggggatt gccggcgttc cggtgctttc tggctccggg
    28381 ttgcgcatcg tggtgtgggt tatcgccacc ctgattggcc tgatctttac catggtgtac
    28441 gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga ccgcttcttt
    28501 cgtgaaaagc aggcggatgt tgaacaacgt ccgtttacct ttggtgactg gctggtattg
    28561 attgtcctga ccgccgtaat ggtctgggtg atttggggcg tgatcgttaa tgcctggttt
    28621 attccagaaa ttgccagcca gttcttcacc atgggtctgg tgattggcat catcggcgtt
    28681 gttttccgcc ttaacggcat gacggttaat accatggctt catcctttac cgaaggggcg
    28741 cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca aagggatttt gctgctggtc
    28801 ggtaatggtg aagcgggtga tgccagcgtg ttaaatacca tcctcaacag cattgccaat
    28861 gccattagcg gtctggataa cgcggtcgcg gcctggttta tgttgctctt ccaggcggta
    28921 tttaatttct tcgtgacatc tggttctggt caggcggcgt taaccatgcc gttactggca
    28981 ccgcttggcg atctggtcgg tgtaaaccgt caggttaccg ttctggcgtt ccagtttggt
    29041 gatggcttca gccacatcat ttacccgacc tcagcttcgt taatggcaac gctcggtgtt
    29101 tgccgggtgg acttccgtaa ctggctgaag gtgggcgcga cactgcttgg actgctgttt
    29161 attatgtcca gcgtcgtggt gatcggcgct cagttgatgg gctaccacta aaaatgttaa
    29221 gagccgcaat tgcggctctt ttttcattct gccgtttcag tctctacggc ttcatttttg
    29281 gcattgcgct tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa ctcatcgcag
    29341 cgtgcggtga tttcttccgg cgtcagccag caaacttcgc tgacttcatc ttcctgtagg
    29401 gcgaagggac cgtgagagac gcagctgaac aacgcgcccc agacacggca atttttatct
    29461 tcgaaataga actgcccgtg ctcggcaaag gggacaccgg caatgcccaa ctcttcttcc
    29521 gcttcgcgac gcgcggattc cagcagttgc tcatcggcct ggactacacc gcctgcggtc
    29581 gcatctaaca tgccgggtaa aaagtctttt gtctcggtac gacgctggac cagaattttg
    29641 cccatgccat catgcacgac gatgtaagtt gcacgatgac gcagacactg tgcccgcatt
    29701 tgttcccggc tggcttgtgc aatgacttcg ttctcttcat tgacaatatc cacccattca
    29761 gtacttgcca aacgacgctg ttccattatc aggaaacctt ctttttctgg cgctcttacg
    29821 gcgcattttg agttgtgggt aaattacgga ttaatcgcga cctgcgcaat gatactttga
    29881 tcattgagtg cgattacact aagtacgtca ttatccagca tgccataact cgccggatta
    29941 ccgcctttag gaatactcac cgagccgggg ttgaagtgga aaatctcccc ccgctgttct
    30001 gccactggca gatgggtatg accgtacacc agcacatcgt tctggtttaa agcaggtagg
    30061 ttttccgggc caaacagatg accatgcgtc aaaaacagac gttgtttttc cagtaatacc
    30121 tgttgccacg gcgcggttat cgggaaatgc aaaagcattt gatccacttc gctgtcgcaa
    30181 ttgccgcgca cagcgataac cttatgtgcc acttcattaa gccgttcagc gactttggct
    30241 ggcgcgtaac cctccggtaa agcattacgc gggccgtgat tcaacacgtc gccaaggatc
    30301 accagccact gggcaccact ttgggcaaac aactccagaa cacgttccgt cgccggtaac
    30361 gacccatgaa tgtccgatgc aaacatcagt ttcatcactc actcctcgtc gaaaaaacgt
    30421 aagctatgat actggattct gtcgttgcta tcagccagat tgcttcgccg aaagagcaat
    30481 aaaacgctgg acagacgctc gctgccactg gaatgttgca taatccacca ggcgttctgg
    30541 cacctcatcg ccatgtagta ccaggcggtt aatcattagc gccagatcag tatcagcaat
    30601 gcaccattca ccaaataaat tcggctgacc cagtgctaac aaatgttctg ccatcgcgaa
    30661 cagtttctct gcactggctt ttccctccgc cgttaatggc gcttttttcg cacccgcaaa
    30721 aacgacatct gtcgaacgtt cttcgcggat gggcatcaga tcgctacgca gccaggcctg
    30781 aatctgtcgt gcacgcgcac gattttctaa atcaagcgga taaatgcgtt cccaggtcgg
    30841 tggcgcaaat cgatcttcca gatactccgc aatggcagaa gattcactca actcaaaatc
    30901 atcgatttgt aataacggca cacggcgtgt ctgaccgtaa ccttgccacg tcggctgcaa
    30961 atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt tttcctgcaa
    31021 cgccacccag gcggataaca cataagggga gaaaaaatgg gcatctgacc aaagcgtgat
    31081 agcgggttta ctcattatga cctcggcaat aaagggggct ggctatcaaa ctatagagcc
    31141 tttttgtcac tgtcacgtga cgaatactca cgcaagtcgc cgcgctttct atacttgtgg
    31201 tgttcagatg attagacaac cggagctgca atgatcgatc tctatttcgc cccgacacca
    31261 aatggtcaca aaattacgct gtttctcgaa gaagcagggc tggattatcg cttgataaag
    31321 gtagacctgg ggaaaggcgg tcagtttcgc ccggaatttt tgcacatttc gcctaacaac
    31381 aaaattccgg caattgttga tcattctcca gccgatggcg gcgaaccgct aagcctcttt
    31441 gagtctggtg ccattttgtt gtatctggct gagaaaacag gactcttttt gagtcatgaa
    31501 acgcgtgagc gcgccgccac attacagtgg ttattctggc aggtaggcgg actggggccg
    31561 atgcttgggc aaaatcatca ttttaatcac gcagcctccc aaaccattcc ttacgctatt
    31621 gaacgttatc aggttgaaac tcagcgtctg taccatgtac tgaacaagcg gctggaaaac
    31681 tcgccctggc tgggtggcga gaactacagc attgcggata ttgcctgctg gccgtgggtt
    31741 aatgcctgga ctcgccagcg aattgacctc gcaatgtatc cggcagtcaa gaactggcat
    31801 gagcggatcc gttcgcgccc tgccaccggt caggcactgt taaaagcaca actcggtgat
    31861 gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt atttcatcct aagaaaaaca
    31921 acggagaacc tgcaatggca caacctgccg ctattattcg tataaagaac cttcgtttac
    31981 gtacgtttat cggaattaag gaagaagaaa ttaacaaccg tcaggatatt gttatcaatg
    32041 tgacgattca ctaccccgcc gataaagcgc gcaccagcga agatatcaac gatgcgctga
    32101 attatcgcac cgtgacgaaa aacattattc agcatgtaga gaataaccgt ttctctttgc
    32161 tggaaaaatt aactcaggat gtgctcgata tcgcacgtga acatcactgg gtgacgtatg
    32221 ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta tccatgacct
    32281 taagctggca gcgttaaccg ccatatcggg aggctgcatg aagatagtga tcaccggagg
    32341 gacaggatta attggtcgcc atttgattcc acgtttgctg gagctgggcc atcaaatcac
    32401 ggtaatgacg cgtaacccgc agaaagccag ttctgttctc ggccctcggg tggcactatg
    32461 gcaagggctt gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca acctggccgg
    32521 agaaccgatt gctgataaac gctggactca cgagcaaaaa gagcgtctct gccaaagccg
    32581 ctggaatatc acgcaaaaac tggtcgattt gattaatgcc agcgacacgc cgccgtcggt
    32641 actcatttcc ggctcggcaa cgggctatta tggcgactta ggtgaagtgg tggttaccga
    32701 agaggaaccg ccgcataacg aatttactca taaactctgc gcccactggg aagaaattgc
    32761 ctgccgggcg caaagtgaca aaacgcgagt gtgcctgctg cgtaccggcg tagtgctggc
    32821 accggatggc ggtattctcg gtaaaatgct gccgccgttt cgtcttggcc tgggcgggcc
    32881 gattggttcc ggtcggcagt atctggcctg gattcatatt gatgatatgg tcaacggcat
    32941 tctctggctg ctggataacg agctgcgcgg gccatttaat atggtttcgc cctacccggt
    33001 acgcaatgaa caatttgccc atgcgctcgg tcatgctctg catcgcccgg ctattttgcg
    33061 cgtccccgca accgccattc ggctgttaat gggcgaatct tcagtactgg tattaggtgg
    33121 acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt gcgtttcgtt ggtacgattt
    33181 agaagaggcg ctggcggatg tcgttcgctg atgtggttta ctgcaaacat ccgccagtta
    33241 actcccggtg ttacagggtt agtggctttg cgcgataaga tcgtctggtg aaagtcgggt
    33301 caccatcata actaactctc ggtctaaacc tctctccagc atctcctgag caatacgcag
    33361 ggcttcttcg cgtttgccaa gaattaatcc atcattccgc ccttcttcac gtaatctgtc
    33421 agcaatagtc atcagtttct ccttttcttg tggtgcgcgt tccgctatct caccaataaa
    33481 tgcacggaaa cgctgggcat cccccgtttg taatacgtaa ttaaacaggg cttttagctg
    33541 tctgtcatta gtgttccctg taactagcag cgaaacaatt tggtcgacta atcccaacag
    33601 atcgcgctga cgaatatgtt tctgaattaa ctccaacagc gccattttgc gatgttgcat
    33661 aatctcgtca tccggcacca cggtaatatc taccagcgga aaagccgatg aataaatttt
    33721 gcgggctata gcaggctcgg caaattcatc aagccagcaa agtgaataag gataaggact
    33781 tctgcaacca tgataaaaca gcatcgggat caccaatgga agctctttat agcctgcatc
    33841 aagatggttt tgcatcgccg caatggaata gcgcatcatg cgaaaagcca ttaattcttc
    33901 cggcttactt tggtgctcta tcactacata aatataacct gctccctcct gcgttttcac
    33961 agaccacaag aggtcggaat aatattgccg caggtcttca tcaataaaac tgtttggttc
    34021 cagttttagc gttgttaaat cacacagttt gcgcagcggc gcgggaagat gaatatcaat
    34081 aaaatcccgc gcggtgtctg gatggcgtaa aaaagattta aataccgcat catgcggcgt
    34141 ggaagttgtc gatattgtca tggcgttccg tcacccctca aatttaagat gacgcgacaa
    34201 taaccacgat taatacccaa tgcagcaggc agtttttatc tttggacagc gaattcagaa
    34261 ctatttttag cggactgcaa cttttacatt tcagcggaag ctgactcgca aattcccctt
    34321 gaaaatactc cggcagcgtt agccaccgga gggggaatta tttcagcgat cccttaagga
    34381 actgttgcag acgagggctt tgcgggttac caaataactg ttctggcgcg ccctcttctt
    34441 ctattttccc ctgatggagg aaaatgacgt gagtagaaac atggcgagca aagcccattt
    34501 cgtgagtcac taccaccatg gttttcccct cttctgccag ttgctgcata atacgcaaca
    34561 cttcgcctac cagttcagga tcgagcgccg aggtaggttc atcaaacagt aaaacttccg
    34621 gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg ctgaccaccg gacaggtgca
    34681 ccgggtattt cccctgcgca cgttcgtcta tcccgacttt tgccagatat ttcaccgccc
    34741 gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat cggcgcttcc atgacgtttt
    34801 ccagcaccgt catatggctc cagagattga agtgctggaa taccatcgtc aggcgtgtgc
    34861 gcagtaagcg cagctgattt ttatcggcga ctttgagttg accatctttg tcgcgcacca
    34921 gattgatcgt ctggccattg accacgatcg atccttcgct cggtttttcg aggaagttaa
    34981 tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc gatgatgcta attacatctc
    35041 cggcattcgc ttgcagtgaa acccctttca gcacttcgtg ttcgccgtag cgtttatgca
    35101 aatcgataac gtttaattta ttctcggaca tcgtattctc agtgcgttga agaaggtttc
    35161 acatgctgca accagcgttt ttccgctctg cgaaagaggc tgatcaggac ataagagatg
    35221 attaaataga gcaccgcggc aatgccgaag gcggtaaaag gttgatatgt ggcggcgtta
    35281 atatcgcggg caattttcag cagatccggc accgtggcag taaatgccag cgcagtagag
    35341 tgcagcatca ggatcacttc gttgctgtac gccggtaacg caatacgcag cgcagaaggc
    35401 aaaataatgc agcgatacat tttaaaggtc gagaagccat aggctctggc ggcttcaatt
    35461 tccccatgcg gcactgaacg gattgccccg gcaaaaatct cggtagtgta agcgcaggtg
    35521 ttaagcgtca gcgccagcac ggtacagttc agaccactgc gaaaaaaggc gttaaggaac
    35581 tcggttccct taacaatctc cagcgtgtac atgccggaat agaacaccag caactgaaca
    35641 tacagcggcg taccgcgaaa aatataggta aataaccaga ttggaaactg gatgtattta
    35701 ttacttgaga cgcgaccaat cgccagaaac agcgccagaa ctccgcctat cactaccgac
    35761 aaaataagca gccacagggt gatcgccacg ccagtaaagc gataaccgtc ggtccacagc
    35821 agtggtttcc agtattcatg taagatttcg atcacaggtc agccctcttc acacccacgg
    35881 agtagcggcg ctcaaggaac agcagcacac cattggaaac agtggtgaaa accaggtaaa
    35941 tcacgccaca gacgatggcg aaatagaacg gttcccaggt acttttgcct gccagctgag
    36001 tggctttgac cacatcttcc aggccgagta acgaaacgag tgcggtagat ttgaggatca
    36061 cctgccagtt gttgccaatg cctggcaggg cgtaacgcat catcgccgga aacatgatcc
    36121 gtcgaaacac ttgcccacga gtaaaaccga acgccgtcgc cgcctctatg tgtcctttcg
    36181 gcactgccat aaacgcgcca cgaaacgttt cggtaaaata agcaccgtag ataaaaccga
    36241 gagtgataat acctgcgacc atcggatcaa tatcaatctg cccgacgccc atcgcctccg
    36301 tcaccgtgtt tagcgcaatc tgcaaaccgt agaaaatcag cagcatcaac accaagtccg
    36361 gcacgcctcg aatcagcgtg gtgtaccctt cgaaaatcag ccccgaaagc cgattttgcg
    36421 agagcttacc gccagcgcca attaaaccga taattacagc gagcactaca gagctgatag
    36481 ccagctccag cgtgacgagc gcaccctgta aaataacacc tgaaaaccca tacaacatgc
    36541 tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc tccctccggt tacccgaagg
    36601 gagcggactt tctttcaccg atggcgatta accaccataa acatcaaaat cgaagtactt
    36661 tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg gcaaaggctt tgttcagtgc
    36721 ctcgcgcagt tcgttatctt ctttacgcag gcccatgccg gtccctacgc caaacagttt
    36781 ttcatcttta acagacggac caccgaattt gtaatcttta ccgacgggtt gtttgaggaa
    36841 accttcgctg gcagcgacct catcctggaa cgcggcatca atacgtccgg cagtcaggtc
    36901 agaataaatg ttgtcctgcc cctgataaga aacgatttca atgccttttg gtgcccaatg
    36961 ttcgttaccg aacgtctcct gggtggtgcc ctgcaatacg cctacccgtt tgcctttcag
    37021 cgactcgact gtcggctgaa tgtcggaatt tttcgccacc accagacgag aatcggcagc
    37081 gtacagtttg tcggtgaagg caatttcttg ctgacgtttt tccgtaatgg aaagcgatga
    37141 catgatggcg tcaatcttct tcgcttttaa ggacgggatt aacgcatcca gtgggttttc
    37201 gacaaacgta cattgcgtat tgatgcgttt gcacaactcc ttcgccagat cgatatcgaa
    37261 gccaaccagt tcgccttgtg aattttttga ttcaaatggc gcataggtcg gatcggtacc
    37321 gatgcggatg ttttgcggaa tcgcagcaaa cgccgcagtt gcgctggaga aggccagaac
    37381 cagagagagc gatagcacca gttttttcat aatgtcctca actgacagtc tttttatagg
    37441 gaatatttac aggtttgtta cacttatcgt gccataaaat tgaccatcaa ggcaaaatat
    37501 cccgccccgt tagacgtatt atttttcatt aaaaatgctt aagtatgcat ttcaatgcac
    37561 cacgatagtg caacaaacct ggcgtgcacc atgatgatca ttccatcagg tacagtttcc
    37621 ctgcgacgta tcagtcaccg tagacattaa agtcgaaata ctttttcgcc atcttgtcgt
    37681 aggtaccgtc ctgacgcagc tcgccaagcg ccttattaaa cgccgccgtc agttcagcat
    37741 catctttacg tagccctaca ccggtaccgt caccgaagta ttttttgtct ttcactgatg
    37801 agccagcaaa ggcgaaatct ttaccagcag gttgcttgag gaaaccttcg ctggccgcca
    37861 cttcatcctg cagtgcggca tccagacgtc ctgcagccag atcggaatag accaaatcct
    37921 ggttggcata ggccaccaca tccacacctt tgctacgcca ggtctcgtta gcgtaagctt
    37981 cctgggttga tccctgcagc acacccacat gtttaccttt cagtgaatcc agcgttggct
    38041 gaatcggcga acctttggcg gcaatcaaac gagaatctgc ggcgtacaac ttgtcggaga
    38101 aagcaatctc ctgctgacgt ttatcggtaa tggagagcga cgaaataatg gcgtcgattt
    38161 ttttcgcttt cagtgagggg atcagcgcgt caaagtcact ggcaacccag gtacatttca
    38221 cctgcatccg tttgcacatc tcgttaccga gatcgatatc aaagccaaca aagtcacctt
    38281 tggcatcttt cgatgagaac ggcgcatagg tggtatcggt tccgatacgt accgtctccg
    38341 gtagcgccgc atagctggaa gccactgcgg agagaccgac taacaaagac agagcgagaa
    38401 tcgacttctt catacataac cctcaagtga aaatggcttt tttatgttgt gtattgtgtt
    38461 gtgtgtttgc aggctctttc atgcaggtct tatgccatct ttacgacaac agtaacattc
    38521 aacgttaaat atgttaataa gacgttgcat tattgtccta aagttgaaga tagcaggtat
    38581 ggcggttgaa tcgcagcgtt tcgattcatc gaaaggaaca aaatgtcgag gatttgatcg
    38641 cggttgcaaa attgccctga aacagggcaa caatgaggtt atgcgccctg ccagcgggca
    38701 aagagatctt caggaagggt tatcgcaaac tggtcaagaa cacgattaac cgtctgattt
    38761 atcacatcat caagggattg tgggcgatga taaaacgccg gaacgggagg cataatcacc
    38821 gcaccgattt ctgccgcctg agtcattaaa cgcagatggc ctaagtgcag tggcgtttca
    38881 cgcacgcaga gcaccaacgg gcgacgctct ttcagcacca catctgccgc acgggtcagt
    38941 aagccatcag tatagctatg gacaatgccg gacagggttt tgattgaaca gggtaaaatc
    39001 accatcccca acgtctggaa agaaccggaa gaaatgctgg cagcaatatc gcgcgcatcg
    39061 tgtgtcacat cagctaacgc ctgcacttcg cgcagggaaa aatccgtttc gagggataag
    39121 gtctggcgcg ctgcctggct cattaccaga tgcgtttcga tatctgtgac atcgcgcaga
    39181 acctgtaata aacgcacgcc ataaatcgcg ccgctggcac cgctgatgcc tacaatgagt
    39241 cgtttcataa atagttgccc tgtcagactt gcaggcagac tttgcaggat ttcgacggga
    39301 gttgcaagtc agggtgccag accggcaccc tgagcgaagg catcatcctt cgttatgcat
    39361 ttcgagattt tccacttcgt tctgacgttg cactgctttg gcgtcgtcat tacgtaaagt
    39421 atcgaggaaa tcgaggtagc cctgatcaac gtctttggtg acgtagacgc cgttgaatac
    39481 cgagcattca aactgctgga tatccgggtt ttcagcgcga acggcttcga tcagatcgtt
    39541 cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg cgaatttcat ctacttcgcg
    39601 accgtgagcg atcagttccg tggcgctcgg catatcgata ccgtaaacgt tcgggaagcg
    39661 aatttccggt gccgcagaag cgaggtacac tttcttcgct ccggcttcgc gtgccatctc
    39721 gataatctgc tcagaagtgg tgccacggac aatggagtcg tcgaccagca agacgttttt
    39781 atcgcggaac tcggcgcggt tggcgttcag tttacggcgc acggacttac gacgcagctg
    39841 ctggcccggc atgatgaagg tgcggccaac atagcggttt ttaacgaagc cctggcggta
    39901 cggtttgccc agaatacgag caatttccag cgcgatatca cacgaggttt ccggaatagg
    39961 gatcaccacg tcgatatcca gatcttccca ttcgcgggca attttctcgc ccagtttggt
    40021 gcccatattc acgcgcgcgc tgtaaacgga aattttgtcg atgaacgagt ccgggcgggc
    40081 aaagtataca tactcaaaca ggcacggatt gctgaccgga ttgtcagcac attgacgggt
    40141 aaacaactgc ccttcttcag tgatgtaaat cgcttcgccc ggcgcgacgt cacgcaggaa
    40201 atcaaagccc agcgtatcga gcgcaacgct ttcggaagcg accatatatt ctgtacggtt
    40261 ctcgtcaata tcacgttttc ccagtaccaa cggacgaata ccgtttggat cgcggaaagc
    40321 aaccataccg tggccgatga tcatcgccac acaggcatac gcgccgcgga ttaagcggtt
    40381 tgtagcagcg atggcggcga aaatgttgtc ggcttccagc ggatagtggc ggaagttgtc
    40441 cagttcgctg gcaaagatat taagcagaat ttccgagtcg gaagtggtgt tgatgtggcg
    40501 gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg ttggtcagat tgccgttgtg
    40561 ggcaagcgta atgccatacg gggagttaac gtaaaacggc tgcgcttcag aggcgctgga
    40621 gctgccagcc gtagggtaac gcacatgacc aatgcccata ttgccctgca aacgctgcat
    40681 atggcgagct tcaaatacat cgctcaccag cccgttcgct ttacgcaaac ggaagcagtt
    40741 attggcatct atggtgatga tgccggcggc atcctgaccg cgatgctgaa gcaccgttaa
    40801 ggcatcataa atcgactggt taaccggcat aacaccggcg ataccgacaa taccgcacat
    40861 acgtcttttt cctcgttaag ccacatctca gagcacttac gctctgggca agaaacttga
    40921 cgagctttgc agataatcaa aaaaccatct gatgataaaa ctgaactgcg ggatcagctg
    40981 tgatttgctc cagtcttcgc ttttcgacac cccggtaaag gagtcgagaa agaagagaat
    41041 ggcggcaaca atcaacacac cgcgcaacgc accgaaacag acgcccagca cccgatcggt
    41101 gcctgacaac cccgttttct ctaccaactg gcctatcacg aagttcacga tagcaccaac
    41161 gatcagggta gcgataaaca gtaccgcgat ggcaatccca tttcgaacca gttcgtcttc
    41221 aaagcccgta aaccagactg acaggtaagt gtagtaatga ctggcaacaa agaaagcaca
    41281 accccatgtc accagcgata acgcttcacg aacaaagccg cggatcaggc taaccagaga
    41341 ggaaaaagca atcaccgcga ttatggcgta atcaatccag accatatgtg tcccacgatt
    41401 ttacgccctg tcatcctgtt cggggcgcat tctaacagaa aaagaaaacg tttgcgtagg
    41461 gatttccttc ccgcgcatca ataaaaatgg cgctgaaaaa acattcaacg ccatcgactt
    41521 tttatgcctt tgcggcatcg ggcaatgcgt gtcggatgcg gcgtgaacac cttatccgac
    41581 ctacggttct acccctgtgt aggcctgata agacgcgcca gcgtcgcatc aggcaagacc
    41641 gtattaattc ggcgtatagc ccattaccac gccacttaag ccagaaagtt gcttcaactc
    41701 acccagcgat cctttcagct tatctttcga ggcatcaggc ccaaccagaa tacgggtaat
    41761 tttaccctgc actggcgtgg atggcgacgt ataaacccga taaccggcac cgcgcagctt
    41821 accgacaatc tcattcactt tatcggcatt tttcagcgcg cccagttgca caacataggc
    41881 tttacccgtt ggcgctgctt tttcttccac gaccggcttc gacgcaggtg gtgcttcaac
    41941 cttcggttta ggcgactcca ccggtttcgg cttcggtggg gcgaccggtg caggttccgg
    42001 ttcaaactcg gtgttattga ctgcaatagt ggccggatcg agcgacggtg ctgcggcatc
    42061 acctgcccgc acctcttccg ctgcgccttc cggcggctgc gtcggcagcg cctgggtggc
    42121 ggctggcatc atatcaggct catcgcgatc gcccgctttc ggcaccagcg ggatagccgc
    42181 gaactcatcc tgataatgtt ttttctgccc gtccagcagc cctggaagta caatcacccc
    42241 cagcgccacc agcacgatcg tgcccactaa ccgattctga aacttacttg ccaccgcttc
    42301 tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg gaacgaacca cacaccagca
    42361 cggtatcttc cgctttagcg tccgccattg cggcatccca tgcctgcgcc acgctatcaa
    42421 atgatttgcc gttacccaaa tgctcaagca gttgttctgc cgtggcaccg cgcggccctt
    42481 ccagtggcgc acaataccag tcatcaacca cgcttttcaa ccaggccaga gttccggcaa
    42541 tatctttatc atgtagcata ccgataaccg ccagcacgcg cccgtttttc ggtagcgctt
    42601 tcatacgccc ggtgagatat tccgccgcat gtggattatg cgcgacatca aaaataacgc
    42661 gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc
    42721 gaatggcatt ttcactgacc tccagcccgc tggcacgcag tgccgccagc gccgttgcgg
    42781 cattcggttg cgggacaagc ggcaacggca gattttccag cgtgccgtga gcatcgctaa
    42841 acgcccagtc atgatcggtg acggaatagt tccactcaac gccccgacgt tgtaacagtg
    42901 cacttttttc ctgcgccaca tcagcaatgg tagaaggcat ttccggctca ccgacaattg
    42961 ccggtttttc gctgcggaag atgcctgctt tctcgcgacc aatgctttcg cgatctggcc
    43021 ccagccagtc ggtatgatcc agcgcaatgc tggttactac cgcaacatcg gcatcgacaa
    43081 tattggttgc gtccagacga ccgcccagcc ctacttccag aatcaccacg tcaagttgtg
    43141 cctgtttgaa cagccacaac gccgacagcg taccgtactc gaaataggtc agggaaatat
    43201 caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc ggcaattcct
    43261 ggccctgcac acgtacgcgc tcggtataac gcaccagatg cggcgaagtg tagacgccca
    43321 ctttgtaccc tgccgccatc agaatcgact ccagcgtacg gcaagtggtg cctttgccat
    43381 tcgtacccgc aacggtaaac acaaatggcg caggtttcag gacgccaaga cgcgccgcga
    43441 cctggctcac gcgctcaagg ccgagatcga tagttttact gtgcaggttt tccagataag
    43501 aaagccacga agccagaggc gacgcggctt gaggagtgcg tttgataatc atggtatccg
    43561 ctgattcgtt acggtgagaa tagcaaaagg gcagagccag tggccctgcc cttatcagtt
    43621 atcaggcctc aggttcctga tctggtaccg gtggcaccac tacgccttca cgcggcgctt
    43681 caggattcgg cgctggcaga ttcatcaact tcgccagaat gctcgccagt ttcaggcgca
    43741 tttccggacg acggacgatc atgtcgatcg cgcctttctc gatcaggaat tcactgcgct
    43801 ggaatcctgg cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc
    43861 cgattaacgc tttcggttca gcgatgttga gatcgcccag catggcgaaa cttgcagaaa
    43921 caccgcccat ggtcgggtcg gtcagcacgg agatgtacgg caagccgcgc tcctgcattt
    43981 ttgccagcgc cgcagaggtt ttcgccatct gcatcagcga catcagtgct tcctgcatac
    44041 gtgcgccacc agaggcggag aagcagatca gtgggcagtt atcttccagc gcctgctcaa
    44101 cggcacgcac gaaacgtgca cctaccacag accccatgga accgcccata aaggcgaact
    44161 cgaatgccgc agcgacaact ggcattccat acagggtgcc tttcatcacc accagcgcat
    44221 ctttttcgcc ggtttctttc tgcgcagatg ccagacggtc tttatacttc ttggagtcac
    44281 gaaacttcag cacatctttc ggctcaagct cgctacccag ctccacaagg cttccttcat
    44341 ctaacaggct atgcaggcga ttacgcgctg tcatacgcat gtgatggtca cacttcggac
    44401 agacctcaag attacgttcc agctcagcgc ggtataaaac ctgaccgcag ctatcacact
    44461 tagtccacac cccttcagga atgctcgcct tgcgggtggg agtaatgttg cttttaattc
    44521 gttcaatcca gctcattagg gacctttctg tctgaacctg gttcgatgcc agttttatct
    44581 ttggggacgc ataatgccat ttttgccccc aacagaccat gaatgttgca cattaaaaca
    44641 taacagcccg aaactttgga taaaaaagtg gtcgaaccgc ggagttactt tttattttgc
    44701 ggcgcgtgcc gcagcgcgtt tgtggcgaat aaattcgaaa acacctggca atattgaaac
    44761 aaaaataatg ccaacaatca tcagtttaag gttgctctga atgaagggga gcgttccgaa
    44821 gaaataaccc gcgtaggtaa aaatcagtac ccatagcaac gcgccgatga cgttaaaagc
    44881 ggcgaaatga cgatacgaca tgtggcccat tcccgcaacg aacggcgcga acgttctgac
    44941 gatcggcaca aaacgggcga gaataatcgt tttgccgcca tgtttctcat aaaactgatg
    45001 agtcttatca agataactgc gacgaaagat tttcgaatcc ggattactga acagtttgtc
    45061 accaaataac cgtccaatgg tgtagttcac cgcgtcaccc acaatcgcgg cgatcagcat
    45121 cagcaccacc atcatatgga cgttgagatc gttggtttcc agtgatgcca acgcaccggc
    45181 aacaaacagc aatgaatcac ccggtaaaaa cggcgttacc accagaccgg tttcacagaa
    45241 caaaattaag aacaaaatgg cataaaccca gacgccatac tccgcgacca gttctgccag
    45301 atgcacgtca atgtgcagga taaaatcgat gaggaaataa atcaggtcca tattagccta
    45361 tgccttgtac tcgtcatctt tcaggctgta actacgttgg ctacagcctg aaatgttccg
    45421 agcattattc ttgttagtcc gccagaaata gcgggcccat tggcggtttt ggaagatcat
    45481 accggtcagg gtaatccacc gcgaccagat acagcccttc cgcttttgcc gttgctgccg
    45541 ccagcgttct gtcctttgcc gccagcaact ctgctatcca gctctccggc tggttgtggg
    45601 caccgacttc catcaggctg ccgacaatat tcctgaccat atgatgtaca aaggcattcg
    45661 ctttgatatc taccaccaca taaggaccgt gacgcgtgac attaatatgc ataacgttgc
    45721 gccacggggt tcgggactgg cactgcaccg cacggaacga ggtgaaatca ttctcgccca
    45781 gcaagcattg cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta
    45841 cccctttact cagtaccgcc gggcgcagcc gatgattgta gatgatgtag cgataacggc
    45901 gagccgtggc gctaaatcgg gcatgaaaat catcaggtac agctttaacc caacgcacgg
    45961 cgatgtcacc aggtaaattc gcatttacgc ccaacgtcca cgcagcgtct ttgcgctgtg
    46021 cggtcgtttc gaaatgcaca acctgcccgg taccgtgtac accggcgtca gtacgcccgg
    46081 cgcagaagac ggtgatgggt tcgttcgcca cctgggagag cgccttttcc agcttctcct
    46141 gcacactgcg gacttcattc tgccgttgcc agccgtaata cctactgccg tcgtactcaa
    46201 tgcccagcgc aattttataa actggcggtt gttgctggtc ggacattagt acagatactc
    46261 ctgcaccagt ttctcggcga tttttactgc catcagcgcg ccgccaaagc gaacgttatc
    46321 ggccaccgac cagaactgaa cttgctccgg cataccgtag tcattacgta cgcagccaac
    46381 agaaagatgc ggcgtaccag aagcatctcc tacctgagtc gggaattcgt tctcttcaga
    46441 gagcacaata tcttcgccct gagcaaacgc atcacgcgct tcttctgccg ccagcggacg
    46501 cagtgcttca aagttgacca tctgggcatg accgtagaat accggtgcct ggacgacgct
    46561 tgccgaaatc atcagccctt cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg
    46621 ttcttcacgc acgctacctt cgctatccgg cagtaacggc agcatgttga acgccagctg
    46681 acgcccgaag aaatcttctt cgtcaatcgg aatgccattg agtaatttag cactctgccc
    46741 cgctaacgca tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc tggtaacgct
    46801 gatacgtgat aaaccgccct gatcaattag cggtttcagt gccgccagca gctggctggt
    46861 cagactgtcc ggtacggcga tgacattccg gttgcggtaa tcggtcagta caaacgggtt
    46921 tacttccggc actaccagcg gtacgtcagg ttcgagagca aacaatccgc tgctgtcgat
    46981 caccagacaa cctgagttgg tcgcttcttc aacccaggca gcggtagctt ctttgcctgc
    47041 gacaaaaaat gccagctgcg cctgcgtcca gtcaaattca gcggcatcct gcacggtgat
    47101 tgtcttacca ccaaagcgca gttgttcgcc tgcgctttcg ttacgtgcca gtgcataaat
    47161 ttccccaacc gggaactgac gttcagccag cgtttcaagc agggcttcgc ccacagcgcc
    47221 agttgcgccc aggacggcaa tgttccagcc ttcagacatg gtggtttact ccagaaatag
    47281 caaagctccc tgccaatgtt acagcagaga gcatgaagaa gagattaacg tgccggatga
    47341 tgaacggcgt taaaacccag tttacacagc aatgatgccg cactggcgtc atcacaaatt
    47401 acatacagag atgaccattc acggcgctca agatagtttt tgcgcagttt atcgaactca
    47461 cccggtatcc cggcgacttt acgcagtggc gcatcatcgc ggcgcacatc atacaccaaa
    47521 tgcaccagcc ttttcagcgt cggttgatcg agcggaccat gcagcgtaat gcgaccaaac
    47581 tctggcgcgg gcagtaatgt atccagcgca acgtgttgtt catgcccaat gaatttgcta
    47641 taggcttcaa acacttgcgt agtaccgcgt gctttacctt ccagggtata gcctgcaata
    47701 tgcggcgtgc cgatatccac ttttgtcagc aattccacat taagttccgg ctcgccttcc
    47761 cagacatcca gcaccacgct taacttctgg ccttcattca ggcaggtcag cagcgcggta
    47821 ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg ctccgggctt caggctacgg
    47881 atcagttttt catccgccag atgtcgcgtt ttgtacggac catctttaaa gagtggcgta
    47941 tggaaagtca gaatatccgc atgctggact aattcatcca gcgagcggaa atcgccctca
    48001 tccccacggt cggcgcgagg cggatcgcaa agcaaggttg taatccctaa cgcttccagt
    48061 cgtgcctgta aacgccgacc aacgttacca acgcccacga tccccacggt acgctcgtgc
    48121 agtgaaaatc catcgcgttc agcaagcatc agcagtgagg agaaaacata ttccactact
    48181 gcaatcgcat tacagccagg tgcagcggaa aaaccaattc ccgcctgctt taaccatgtg
    48241 tcatcaacat ggtctgtccc cgcggtggca gtgccaacaa atttaatggg ttttcctgcc
    48301 agcaaagatt cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt
    48361 tgagccacgg ggatgggacg cccgggaacc gcggtcacct cacccaaacg gctaaataag
    48421 tcgcgggcat aaggcatatt ttcatcaaca aggattttca cgtttgtgtt acctgtatga
    48481 gacgagagtt aaccggacaa gtgtgccata atctcgcggc caggcatact tgcgaagatt
    48541 tcaggtataa ggatacgtaa tgatacaacc tatttccggc cctcctcctg ggcaaccacc
    48601 aggtcaggga gataacctgc cgtctggcgc gggcaatcag cctttatcca gtcagcaacg
    48661 tacttcgctg gaaagcttaa tgacgaaagt gacctcactg acgcaacagc aaagagcaga
    48721 actgtgggcg ggtatcaggc acgatattgg tctgtcggga gattcaccgc tgctttcgcg
    48781 tcacttccct gccgctgagc acaatctggc gcaacgtctg ctggccgcgc aaaaaagcca
    48841 ttctgcccgc cagcttttag cgcaattagg ggagtattta cgtctgggga ataatcgtca
    48901 ggcggtcacg gattatatcc gtcataactt tggtcagacg ccgctgaatc agctctcacc
    48961 ggagcaatta aaaaccattc taaccctgtt gcaggaaggg aagatggtta ttccgcaacc
    49021 gcagcagcgc gaggcgaccg accgtccgtt attaccggcg gagcacaatg cgctcaaaca
    49081 gctggtgacc aaacttgcgg cggcaacggg agaacccagc aaacagatct ggcaatcgat
    49141 gctggaactt tccggggtga aagatggcga gttaattcca gcgaaactgt ttaaccatct
    49201 ggtgacctgg ctgcaggcgc gtcagacgct aagccagcaa aatacgccga cgcttgaatc
    49261 actacagatg gcgctgaaac aacctttaga tgccagtgaa ctggcggcgt tatcggcata
    49321 tatccagcaa aaatatggcc tttctgcgca atcatcgctt tcttctgccc aggccgagga
    49381 tattcttaat cagctttatc aacggcgggt taaagggatt gatccgcgtg atatgcaacc
    49441 gctgcttaat ccttttccac cgatgatgga cacgttgcaa aatatggcaa cgcgtcccgc
    49501 gctgtggata ctgttagtcg cgattatcct gatgctggtc tggctggttc gttaaccccg
    49561 acgaaatgac agtatcgtga caataattcc cagcaccgca gagatcgccc cggcaagaaa
    49621 taccgaagag taaccaaacg tggtcgccag cattcccgca agcggcccgg agacgccgag
    49681 ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaacgt gtgacggtac
    49741 gcgtttaacc acctccacgc ccagcgcagg aaagataagc gaacatccgg ctccggttaa
    49801 cgccgcgccc gctaatgcga cccatgcacc tggggcttgc cagagcagca acaagcccac
    49861 cgtttctaca agcagagaga cgatcgccac tttcacgccg ccaaaacggt ccggcatcca
    49921 gccaaacatg acgcgcatca cgacaaatgc gccgccaaac gcggtaagcg taaagcccgc
    49981 catcgcccat cctttgctgg caaagtagag cgaaacgaaa gtcccgataa ccgcaaaacc
    50041 aacgccttgt agtgccagac ctaaccctgg tttccagata agcccgacaa cgctccacag
    50101 cgatggacgt tcccccgcca gggccggtac tttgcgcact gtgccgttac aggcccacgc
    50161 cagtaagggt aatgccattg tggtgagcgc cagtgcggca aaaccgtaat ggctatgaat
    50221 caataggcca agcggcgcac caacagcgag ggcaccgtaa atcgccattc cgttccatga
    50281 catcactttg ccggaatgtt ttggcccaac gatgcctaat ccccaggtca gagcgcccgt
    50341 cagtaactgg ctttcaccaa agccaagaat caaacgcccg acgaccaaca gggcaaattt
    50401 gaacggtgcg gggacgggca aaatcgccgc cagcagcaac gcgccccccg ccagaccaca
    50461 agctaacatc ccctgaagcg ccgaacgttt tgcgccatat tgatcggcca gtcgcccggc
    50521 gtaaccacgc gtcagcaccg tagccagaaa ctgaatcccg acggcaatgc cgaccatagt
    50581 gttgccatag cccagttcat gatgaacaaa cagcgggata accggcaacg gcaaccctac
    50641 ggtcatgtag gtgagaaaaa ccgcaaaagc gatgcggaag agcgaaaaat tggcagaaga
    50701 tcgtgtttcg gtttggctta cagcagtcat gcattactcc agaatgcagc gcaaggcgag
    50761 gagtatcccc gtctcatctc tctggtttca gggttacggt gcgttggcag gaattaacgc
    50821 gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt gcctggtaca ggcggcgatt
    50881 gtcaatgatg tgaaaaaggg aaccataggg ttcccttatt ctttagtacc ggaggcagca
    50941 ttgccgcata tcgtcggatg cgacgctgac gcgtcctatc cgacctactc cgaattaatc
    51001 tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat
    51061 aacggtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc
    51121 gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga
    51181 gtagatagct tcctgtacgc cagcagcgcc cagagagtga ccggtcattg ctttggttgc
    51241 agaaatcgcc gggctcttat cgccgaacac ttcacggata gcagccagct ctttcacgtc
    51301 gccaaccgga gtcgaagtac cgtgggagtt caggtagtcg attggggtat caacaccgtg
    51361 catcgccatc ttcatgcaac gcactgcgcc ttcgccagac ggagcaacca tgtctgcgcc
    51421 atcagacgtt gcgccgtagc caacgatttc agcgtagata tgagcaccac gtgccaacgc
    51481 gtgttccagt tcttcgacca ccaccatacc gccgccgcca gcgataacga aaccgtcacg
    51541 gtgagcgtca taagtacggg aggctttttc cggggtgtcg ttgtatttgg tagacagcgc
    51601 acccatcgcg tcgaattcac aagccatttc ccagcacagc tcttcgccgc caccagcaaa
    51661 cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga
    51721 ggtcgcacac gcggagctga tggagtagtt tacaccgtga attttaaacg gcgtggcgag
    51781 acaggcagaa acgccggatg ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag
    51841 gccgcgcggg ccgcgcattg cgtcagcgcc gaacacctga aaacgcgggg aaccgccgcc
    51901 ggaacctgca atcaggccaa cgcgcgggtt attctggtaa gcttccggag agaggccagc
    51961 atctgcaatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg
    52021 cacaactttg cggtcaatga ggccagtggt atccagtttt acgttgcccc agacgtggct
    52081 acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag
    52141 agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat
    52201 cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca
    52261 atagcgtaca cttgtacgcc gaacaagtcc gatcagccaa ttaatagaga aatttgcgca
    52321 gcctgacaca catcgttaag atcgtcagac tgcccgtcag acgagtaact tacgtgaaac
    52381 actacgccat acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag
    52441 attttgacga cgtttacttt tccaatgaca acggactgga agagacgcgt tatgtttttc
    52501 tcggaggtaa ccaattagag gcacgctttc ctgagcatcc acatcctctg tttgtggtag
    52561 cagagagcgg cttcggcacc ggattaaact tcctgacact atggcaggca tttgatcagt
    52621 ttcgcgaagc acatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat
    52681 ttcccctcac ccgtgcggat ttagccttag cgcatctaca ctggccggaa ctggctccgt
    52741 gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc
    52801 tcgatgaagg ccacgtgaca ctggatttat ggtttggcga tataaacgaa ctgaccagcc
    52861 aactggacga ctcgctgaat caaaaagtgg acgcctggtt tctggacggc tttgcgccag
    52921 cgaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcacga ctggcgcgtc
    52981 cgggcagcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggacg
    53041 ccggattcac gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgtgggg
    53101 tgatggaaca gacattaccc ctcccctgct ccacgccgtg gtttaatcgt acgggcagca
    53161 gcaaacggga agtagcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgt
    53221 tattacggcg cggctggcaa gtaacgcttt attgcgcgga tgaagccccc gcgctgggtg
    53281 cttccggcaa tcgccagggg gcgctgtatc cgctcttaag taaacacgat gaggcgttga
    53341 accgcttttt ctctaatgcg tttacttttg ctcgtcggct ttacgattta ttgcccgtca
    53401 aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata
    53461 aaatcgcaca gatgctgtcg atggatttac ccacagaact ggctgttgcg gtcggggcga
    53521 atgaggttga aggcgttaca ggtgtagtaa caaattgtgg cggtattaca tatccgcaag
    53581 gtggatggct gtgtccagca gaactgacct gtaatgtgct gaaactggcg caacagcaag
    53641 gtttacagat tcgttatcaa caccagttaa aggatttatc ccgtaaggat gactgttggt
    53701 tgctgaattt tgcaggagat cagcaagcaa cgcatagcgt ggtggtactg gcgaacgggc
    53761 atcaaatcag ccgtttcagc caaacatcgt ctctgccagt gtattcggtt gccgggcagg
    53821 ttagccatat tccgacaacg ccggaactag cggagctgaa gcaggtgctg tgctatgacg
    53881 gttatctcac gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc
    53941 gcggcagcga agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga
    54001 ttgattgttt cccgcaggcg cagtgggcaa aagaggttga tgtcagtgag aaagaggcgc
    54061 gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggcaac gtgcccgatt
    54121 atgacgtaac gctcgtggaa tatgcttcgc tggcggagca gaaagatgag gcagtaagcg
    54181 cgccaattta tgacgatctt tttatgttag ctgctttagg ttctcgcggt ttgtgctctg
    54241 ccccgttgtg tgccgagatt ctggcggcgc agatgagcaa cgaaccaatt ccgatggatg
    54301 ccagtacgct ggcggcgtta aacccgaatc ggttatgggt gcggaagttg ttgaaaggta
    54361 aagcggttaa ggcggggtaa tcagctctgg cattgcttgc cggatatggc gtgaacgcct
    54421 tatccggcac attacttact gctgtgacgc cttctgaaac aggttttccc acatatcggt
    54481 caccagcgcc tggtcacgcg gcgacaactc accggcacca atcgcttttt ccagactctg
    54541 gctaacggtt gtatgtaccg cctgggcgga gtggtcatca ccactttcca gttctgcgat
    54601 ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg
    54661 gtcaaccata ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt
    54721 tcctcattgt aaaacgtggc gctggcttca gttgagcgct tccggccacg ggaactgttc
    54781 cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccggacg
    54841 ttcgggaatg ccgtttgcca gataatccat tacctgcgca tgtacccgcc gttgaaacac
    54901 cacgcggtcc ggttcgaaat ccccttccag attgtcgcag ctaacattaa acggatatcc
    54961 cgccgccacg cagaacaacc agtccagcgc ctgcggcttc acttcaacat cttcaaactg
    55021 gctttgcgtc tgggcatcgc gaccgtccgg gcaataccag tagccgaaat caacctgctc
    55081 acggcgcgct tttccggcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa
    55141 gccatgagca aagacgattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg
    55201 ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat
    55261 ctcaatcaac tgctcatagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg
    55321 atctcctgtc cgtggctatc gtaaagtaat tttgcactca tcaccgccga gacgataacg
    55381 atcatcgggc ggatcagctt ttgtcctttg ctcaacacca gtcttgaacc catgcgcgcg
    55441 ccgaggaact gccccaccag catcacaaag cccgtcgccc aaatcacttt gccgccgaga
    55501 ataaacagca gcagaccgcc aatgtttgac gttgcgttga gtaatttggc gtgagccgtg
    55561 gctttggcga ggttgaatcc gcacagcgta acgaaggcca gcgcgtaaaa cgatccggca
    55621 gctggcccaa agaatccatc gtaaaaaccg acgcagcccc ccgccaccag cgcaaatggc
    55681 agtccataca tccggcgctg gcgatcttct tcacccagct ttggcatcag caagaaatag
    55741 aggccgatac aaatcaccag aatgggcaga atctgccgta agacatcagc ctgaacatac
    55801 tgcaccagta atgcgccgct cattgagccg acaaaggtca tggcgatatt gagtttctga
    55861 tcgcttaaac taaccacttt gcggcgaata aagtagatag tggcggaaat agagccgccg
    55921 caggcttgca gtttattggt tgccagcgcg ttagccggag acatccccgc cgccatcaat
    55981 gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg
    56041 gcgacaaaaa agagaactcc cagcagcagc ggggaaacca taaacaggct attaaacgtt
    56101 tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg
    56161 tcttcttctc aggctttgtt gttcccggtt ttggtggttc aaaccagctt tgcagttctg
    56221 ccccgcaacc atcgcccggt ggcggtaaag gttgatcttc acactccagg ctatcggcag
    56281 gacaacgtag tcgtacatgc atatgggcgc gatgctggaa ccagggccgc actttgcgta
    56341 accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa
    56401 aaatgcgcgt gacgtctttg tcctgcgcgg cgagtttaat caagctgaaa atttccggct
    56461 tccacagcgt cgggacaacg tgtttaccgt cgcgggaaac taaatctaac gcttgcgggc
    56521 gcaagagctg cgcggaggtc cagcgtgttt ttggcagttg caggaagata tcgacatcca
    56581 gtccagtctg gtggctggcg tgaccgccgt tgaaacgacc accagcaggc atccccatat
    56641 cgccaatcag caccgtaccc atacccagat tgctcacctg gcgactcaga cgctggataa
    56701 acatcaccag atccgggtgt ccgaaatagc gacgctgatc ggtacgcatg acctgataat
    56761 gttcggactg tatcggcagc gtgtcagcac cgacgataca gccatttgaa aaactgccta
    56821 tcgattgtgc actacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc
    56881 tggcgctact ggcaagcagg gccagcagca caatcgcagt tttgttcatt tttaccagcg
    56941 tggaatatca gtcttcacat cagcattttg cgcccgttgc cgtaacaggt gatccattaa
    57001 aacgatcgcc agcatcgctt cagcgatcgg caccgcgcgg atcccaacac acggatcgtg
    57061 acggcctttg gtgatcatct caacttcttc gccaaagcgg ttaatcgtgc gccccggcac
    57121 agtaatgctt gaggtcggtt tcagcgccat atgggcaatg atttgctgcc cgctgctgat
    57181 accaccaaga atgccgcccg catggttgct ctggaaaccg tctttggtga tttcgtcacg
    57241 gttctggctg ccacgcagcg ctaccacgtc aaaaccatca ccaatttcca cacctttcac
    57301 cgcgttaatg ctcattagcg catgggcgat gtcggcatcc aggcggtcaa aaaccggctc
    57361 gccaagtccg gcgggtacgc cactggcaac gacggtgact ttcgcgccga tggagtcacc
    57421 ctcttttttc agcgcgcgca tcagttcatc taacgcgtcg attttgtccg ggtccgggca
    57481 gaaaaacgga ttttgctcga cctgcgacca gtctttgatt tccagcggaa tatcacccat
    57541 ctgggtcagg cagccgcgaa tctcaatgcc aaatttctcg gcgagatatt ttttggcaat
    57601 cgcccctgct gcaacgcgca tagcggtttc gcgggcggaa gaacgtccac cgccgcgata
    57661 atcgcgcagg ccgtattttt gttcataggt gtaatcggca tgacctggac ggaaaacgtc
    57721 cttaatcgca ctataatcct gagaacgctg gtcggtattt tcgatcaaca atccgatgct
    57781 ggtgccagta gtaacacctt caaaaacacc ggagagaatt ttgacctgat ccggctcgcg
    57841 gcgctgggtg gtatagcgcg atgtcccagg tcgacgacga tcaaggtcat gttgcaagtc
    57901 cgcttccgtc agcggaatgc ctggcggaac accatcgacg atgcagccga gcgccagccc
    57961 gtgcgattcg ccgaaggtgg ttacgcgaaa gagttgtcca attgtgtttc cagccatcac
    58021 ggctccgtta ttgttgtgtt tgcgtgttta cttaatcttt ataaatcgcg aaatgttctc
    58081 gtgcggcaat aagctgctct ttagtgagca taaacacacc atcgccgccg ttatcaaact
    58141 ccagccaggt gaacggaaca tccggatatt gttccataag atgtaccatg ctgttgccca
    58201 cttcacaaat cagcacgcca tcatcagcga ggtaatctgc cgcgttacca agaatgcgac
    58261 gcgtcagttt caggccgtca gtgccagatg ccagaccgag ttccggctcg tggcggtatt
    58321 cgtttggcag gtcggacata tcttccgcat cgacatacgg cgggttggtg acaatcaggt
    58381 cgtactgcac tttcggcaaa tcacggaaca agtcggaacg aatcggaatg acgttgtgga
    58441 tcaaaccgtg ttcttcgatg ttctgttcag caaccgccag cgcgtcggga gagatgtcta
    58501 ccgcgtcgac ttccgcttcc gggaaggcat aagcacaggc aatggcgatg cagccgctac
    58561 cggtacacat atctaaaatg tgctgcggtt gcttgctgat gagtccggca aatttattgt
    58621 tgatcagttc accaatcggc gagcgcggca ccagcacgcg ttcatcgacg taaaattcat
    58681 ggccacagaa ccaggcttta ttggtcaggt aagccaccgg aatgcgttcg ttgacgcggc
    58741 ggatcacgcg ctcaacaata cggtgttttt cgctggaagt cagacgcgcg gtgcgcatat
    58801 cttccggaat atccagcggc aggtagagtg aaggcaacac cagttgcacg gcttcatccc
    58861 acgggttatc ggtgccgtgg ccgtaccaga tattcgccgc gctgaagcgg ctcaccgacc
    58921 agcgcaacat gtcctgaatg gtttgcagct catttactgc ttcatcaacg aaaattttat
    58981 ccacgtattc ctccagggca tgatcgcaat aatttcggcg gctagtttgc catgaagatg
    59041 acgataaatc agcattcacg cgcggtgagt gaggaaaaat acgtttaaaa cgatcgattg
    59101 tgctacgagt caggtaaact gtaggaaaat tagaaataga gacagttaaa tgaaaaagaa
    59161 aacaacactt agcgaggagg accaggcgct gtttcgccag ttgatggcgg ggactcgcaa
    59221 gattaagcag gacacgattg tccaccgacc gcagcgtaaa aaaatcagcg aagtaccggt
    59281 caagcggttg attcaggagc aggccgatgc cagccattat ttttccgatg agtttcagcc
    59341 attattaaat accgaaggtc cggtgaaata tgttcgcccg gatgtcagcc attttgaagc
    59401 gaagaaactg cggcgcggtg attattcacc ggagttgttt cttgatttgc acggtctgac
    59461 gcagttgcaa gccaaacagg aactgggggc gttgattgcc gcctgccgcc gtgaacatgt
    59521 gttttgtgcc tgcgtgatgc atggtcacgg gaagcatatt ttgaagcagc aaacgccgct
    59581 gtggctggcg cagcatccac atgtaatggc atttcaccag gcaccaaaag agtatggtgg
    59641 cgatgcggct ttgttggtgt tgattgaagt ggaagagtgg ctgccgcctg aattgccctg
    59701 aatgctggca tcgaatgctg tagagggaaa gttcgcctta gccatcatat cataaaacag
    59761 acgccggaaa tgacgacagg gccagatggc cccgtcgact tgtgaaaata tcgtcactac
    59821 aattcattca gtacatactg gaatcggcac gtagaatcaa tcggttgtcg gctactggtg
    59881 ttcacattta ccacgatatc atgtagctgt ttatcggtta ccggatgccg tgtgggagaa
    59941 accgttaccg ttgatttatc gcaaccgtag gaggtaaagg ttaacagttc cgagaccacg
    60001 cggcagtatg cacctctgct atcaaaaacg ctgccatcac agagtttgcc atcttccatc
    60061 aggtaggtcg aaagcgtggt ggtgattgtc gagccattgg attgcaactg taacgcctta
    60121 ttgtggctga cgtaattctc actccacgag atgtcatacg acgcgacagg gatcgtcgga
    60181 catactgtgg catggttcgt tggcccagta tatctcccga atccgggttg atctgtgatc
    60241 gtataaaaag aagcgagcca cgggcaacca taactgtcgg actgaacggt tatcttaaac
    60301 gtctcatatg cacactgaaa tgtcgccgta tttatcccag gacaattcga gctatcagca
    60361 ttaccgtagt cagttaagat caacactggc gctggtcccg accccgtcca cgaaataacg
    60421 ctggcttgct cgttcttcct tatggcatcg ttgtacaccc cataggccgc ctcgttggga
    60481 gttaacaccg tgacatacaa acctccggtg ttatgaatag acattttccc cttcccataa
    60541 taaatcttca cactgtcgaa actcgcgctg gtgagtttat ccccggcata gctctttgga
    60601 acgctcgcac tcatcaccag caacaccagg ctcgcaaaaa tcattgtcca ttttttcatg
    60661 ttaaaaatac tcaaaggttg ccgtcgccgt agcgctgaaa tcaccggacg tcacatcccg
    60721 cccgcccttc gcctgtagcc aggcattaaa attgagactg ttattgccct ggtttagcgg
    60781 gaacgtgaca cccgttgtgc tgttgactgg caccgccgcg cctgccgccg tttcaatacc
    60841 aatcccagct ccggatgcag aagatgaggc gtcaaaggcc agaaatccgg gcagttcgct
    60901 atcttccgtt cccgtcagcg ttaccctgac gctaaacagc gtggagcttt tgcaatcctt
    60961 caattgaaat accaacggta ctcgctctga ctgcccgagg ttcattaaat cgctggcggc
    61021 aatggtggga aagtgcacct ccgccaacag gctaccgttg atcactggcg tacaggattt
    61081 accgagcaaa ttaccggtaa agtgcaggtt gtcgaccgcc ggggcagtaa cgctgtacaa
    61141 cagcatcagg ctacagataa aaaccaacgc acgctttttc ttcattgata atccacctgt
    61201 aacgtggccc aggcttcaaa gtctgcctca ggcaaatcga caccgctctt tttgaccggt
    61261 actgcggtta acaccggtaa ggccgtttca ttcaccacca gtgccgtatt gagcttaaag
    61321 ctctgccctg cctgtttaag ccgaatcgcc aggccagaga tatcggtgct aaccgcggca
    61381 tcgtcaaagt cgctctgcgt accgctccag ctcaatttca tttccattga tgggtcgcga
    61441 accgccgtat cacaggtaat ggaatacggc acggtttgct catagttact gccattaatg
    61501 tcatcaacaa aaacatcctg aaaggttatt tcaatggttt taccgttgct aatagtgcag
    61561 ttaggtggct gcactaaata gccgtgaaaa gagacatttg agagtgccat cgagctaaag
    61621 ccccacagca taagaagtga gatccgtttc attggtaatc caccacaatt gtggcactgg
    61681 cgttaaattc cgctgccgat aaggttgtac cactctgttt caccggcact gcttcgagtg
    61741 cagggacgcc cgaggaaaaa ttaaacggca accatgaacc agacgttaag tcgagtgttg
    61801 agtgatcact acttttctga acccgaatac caaaccctga aatacctgtg ctgataactg
    61861 tttcaccgtt gatcacaacc gttgttgccc gcatctgtaa acgcaggtca tccattgcca
    61921 ggttgttaca aacaagcgag taatcaatgg gttgtttata acctacccca tcgatcttat
    61981 tgatgaatac attaccaaac tcaaccgtcg caccattaac cgtgcaagga ggaggggcat
    62041 caacgagcac tcgtaaactc aaatttactg atttactccc tgcaactgca aagtcgctac
    62101 caatacaaca catcagtaac catgttcccg cccagactga acaagatctt ttcatactta
    62161 cttccccgtc attaaccttt gcggctcagc tcttcatcaa cctgacaact gttgccgcta
    62221 caggtgaaaa agagtggcat ccgtgcgcca tagtcgttga cataggtcag caccggcacg
    62281 ctagccatct tcaccttgag cggtgcggag gtttttggcg acatcactat cggcgagaaa
    62341 ccgtctgccg gattgccatt tttttgcgcg ctggcattac tgaaaatgac gtaatacgga
    62401 gtagggttat tgacggtaaa ttcctgaccg ttacgggtta aggtcacttt atgctgccat
    62461 gggttttgca tgctagcttt ttccagggct ttaggtcgcc agaaaagttt gatgcgtgtc
    62521 tgcagcgcaa tttgtaacgt attggctttg ttcgatttcg gcggaatttc acgcacattg
    62581 aaataaaata tcgattcacg atcggcaggc aatttatgga tatctggcaa tccctgaatt
    62641 ttcacctgac cattcatcat tgcatcaatg cgctgtacgg ggggcaatac tgccagcggc
    62701 gaactaatct tgacgccctt ttcatcttcg atccagcttt gtgccagata aggctgtttc
    62761 gggtcgttat tacgcagcgt gacgctgatg gatttatcgc tttcattaaa aatcaggcga
    62821 gtacgatcgg gcgttactga cgccagagca gaaaaactta ccgttgcagc cagtaacgac
    62881 agtaccgttg ccaatgtttt gacagagaga tgtttagtca tggatcattc cttaatcacg
    62941 ttattggttc gatgaaacag acatcggcgg ttccattggt gtcacctgtt gtgtctgctc
    63001 ctgaattaca ggttgaatat catgcgttat gacgggagca cttccccctt tttgctggca
    63061 tggcaacaac aagccattga acaggtcatt cggtaacgga tctggcaggc tgatatcgca
    63121 gtgagattct ccctcccaga aaacggtcat atgttcaccc ggcttaacgc ctgcaagata
    63181 aacattacct tcgtcatcaa ccagcccaac ttgttgttga ttatcgtttt tcacttccgc
    63241 cccaaatggc ggatggctgc catcctgcaa gcgcagcact accatcgcct tctcaccgct
    63301 gatcaccgca aatttacggt agccaatagc gccttccgtc agcgttgcct ggaccaccga
    63361 ttgggtagct tcggcgtttt ccggtagttt gttcaaatcg atataagcct ggttacggta
    63421 gtaatcatta acatctgcca ccaccgcttt cccgaacatg ttggtataaa cggcggaacc
    63481 attaccttcg accggcacat tagcaacgcc atcggcgtcg attagcagac gtgtgcctcc
    63541 catattttgc gtacgatgca acgccccgcc gtgagcagtc agtgttacac cgccctgtaa
    63601 ggagaccccc gcagatgtgt attgcccctc atggtagttt gcactcaggt caacttttgc
    63661 cagcgtactg tcatgactgt aataaccatc aagactggtg tgattttcac tggttccggc
    63721 gctcaacgta tagtggctag cgtcatcgat gcggtgatac aaactcgcct ggctgctgtc
    63781 ggcaccactg ccataattgc cgttgtaact gatggtgctg ttgtcgcccc atggcaaact
    63841 caaggacaga taaacacctt tgtcggcact tttatcatat tcgtagcgat aaccggtgag
    63901 agaaacgctc aggttgcgaa gacttcctaa attgaaataa tgagaaagca tcatgttgta
    63961 gttggtctga tcctcgcgat cccaataggt atggtgagaa taattgagat agaccgaaac
    64021 gcgcgcatcc ctaaagttct ggttgtaagt gaccgtgtac atttccttgt cgttacccgt
    64081 gcgcgcacgg tcggtgtcgt tcgcatccag atactcactc atggtcatgt agtttttttc
    64141 agagaagcga tatccggcaa acgtcacccg actgttgagt tcatcgaaat ctttggcata
    64201 gctgacgcga taagagttgc cgtccagcgt tttgttgcca tacacgctgt tatcgtcgag
    64261 ttgaacacga gagtgagtga catcaaacgc tagcgctccg aacagcgcga ggtcacgacc
    64321 gaggcctaac gctgccgact ggtagttttc atcggccaac gccccgccgt agagcgacca
    64381 gccattcgca atcccccatg acgcctcacc gcccgagaag aagctgcctg caacctgatg
    64441 gtcccagttt tgtggacgcc ccattgtcac tttgtaacgc acctgaccgg ggcgggtcag
    64501 aaacggcatg gacgccgtgc tgacgtcata ttcctgtacc tgaccgtttt gttcttcaat
    64561 gcggacatgc agcgtgccag atacggaatc gccaatatcc tgaatgcgga atggacctgc
    64621 gggcacttgg gtttcgtaaa tgacacggcc catttgggtt acggtcactt tagcggtagt
    64681 gcgtgcaaca cctgaaatat cgggtgcgta gccgcgcatg tttggcggta acatttgatc
    64741 atcggtactg atgctgccgc caatgtaact gaagccatca aaaatatcgg agttgagata
    64801 atcttcaccc agcgaaagtt tggcttttaa tgacggcaag gcgcgccagg cgtagtaacg
    64861 gctccactcc cagttttttt gcgtaccgct atcatcagtg tcgccgtcat cgttactgcg
    64921 ggtatgttgg taatcgctct gccagtcggc tcgtaagcgc cacggcccca cgttaacgcc
    64981 gaccgtacca ttaccgctga tgtcgttagt gtcatccccc ccattttcat gtcgggtttg
    65041 tgcgttgata ctgtaatcgg caataagccc aggaatcccc tcatcccaac gcgaaggtgg
    65101 gtcccagtca tcgtcagtgt attcgagata tgcctgtggc acagaaacta agagcgcaga
    65161 ttgacttaaa tcgcccttaa tttccatacc tcctatttgg tcagttttca gacattgtcc
    65221 atcatgaccc cattgcaaac tcttcgcaaa atcatctttg aagccaaact gggctatcag
    65281 ttcaggtggt aagcaggcat atgatttatt cggatcgttt tcagctacat accaatagat
    65341 atcgtattca tcaggtaatg ggtgattatt tacatgaaca cgtaaattgt atttccctgg
    65401 ttcaacatac cctttttgag aaaaacgccc cagatcaatt ttggtatttc cttttaactc
    65461 cagaaatcga gcatcaaatt gaatggtatc atctgccagt acattaaaag gactcccgga
    65521 aattgccaga gcaatccacc atgaaagccc ccgtaaacga agaaaagaat gttcaggcat
    65581 ctacgtatcc atccatgatt attataattt agttatcttt tatttataaa ccccataaca
    65641 ttgcagttat gggggaacag cataattaga gatatgtaat ctggaacgtc gttagcgttt
    65701 caaacggcgc tggggttggt gttaccgctg cagtttcacc tactaaccag gctttaaagt
    65761 tcaacgtttg tttctgtttg ccttttcctg ttgaactgtc catcgcgatg tcctgatcga
    65821 tgcctgcccc caggtcataa ccagtaccac gttggtcgcc aatcaccaga cttacatttg
    65881 gaatttcagt tccattatcc atgttagaca gggaatagtt ttttgagttg ccggagtacg
    65941 gattaccagt gaatgtcgtg gtcattgtcg tttcagtatt gaaattacaa tcctgtaaac
    66001 gaatctggaa atctttaggt gtggtagtct tgccaccggt taaagaacct gtaccaatgc
    66061 tgcccatatc cacttccagt ttatgatcgt caggaacaat cgagcacgga gagtcttcaa
    66121 ttgtgccgta aaatctcact gtaccgttgt tacctgcggc aacgactgat gctgaagtca
    66181 gcgcccccat gagcattgcc gtggcaactg ctgcttttac aaacttactc attatttgtc
    66241 cttaaatcat aaataattag aatataagcc aactgaaata ttgacacaga acttcttcaa
    66301 tttttgcagc gacttttaag cagccaatga acagattttc agctcctgaa aaaacaggtt
    66361 ccttgatata atattctttg gcgtgccgaa catattgata ttttaatatg actgacgcaa
    66421 tttagatttt ctaacattca aagttagaaa tgcttaatta ataggcgggg ataataatta
    66481 ctgaaatttt tcatctcagt tatgaagaat gagttcaaaa aaacggcaaa tgcaaaaatt
    66541 cagtgaataa atcaaagtca aaaa
//
LOCUS       DAZGAP010000002        65899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGAP010000002 DAZGAP010000000
VERSION     DAZGAP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.002"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..441)
                     /gene="folK"
                     /locus_tag="ACHLAM_000065"
     CDS             complement(<1..441)
                     /gene="folK"
                     /locus_tag="ACHLAM_000065"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO7851658.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEA"
     gene            complement(438..1835)
                     /gene="pcnB"
                     /locus_tag="ACHLAM_000066"
     CDS             complement(438..1835)
                     /gene="pcnB"
                     /locus_tag="ACHLAM_000066"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO7851659.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1895..2791)
                     /gene="gluQRS"
                     /locus_tag="ACHLAM_000067"
     CDS             complement(1895..2791)
                     /gene="gluQRS"
                     /locus_tag="ACHLAM_000067"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO7851660.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTLFTDQLRGIIHANEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNTQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2858..3313)
                     /gene="dksA"
                     /locus_tag="ACHLAM_000068"
     CDS             complement(2858..3313)
                     /gene="dksA"
                     /locus_tag="ACHLAM_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO7851661.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3491..4195)
                     /gene="sfsA"
                     /locus_tag="ACHLAM_000069"
     CDS             complement(3491..4195)
                     /gene="sfsA"
                     /locus_tag="ACHLAM_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO7851662.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTSC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4210..4740)
                     /gene="thpR"
                     /locus_tag="ACHLAM_000070"
     CDS             complement(4210..4740)
                     /gene="thpR"
                     /locus_tag="ACHLAM_000070"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO7851663.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4814..7243
                     /gene="hrpB"
                     /locus_tag="ACHLAM_000071"
     CDS             4814..7243
                     /gene="hrpB"
                     /locus_tag="ACHLAM_000071"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO7851664.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVAHFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPTVNLQAAKRL
                     LQMLGALEGERLSAQGQKMAVLGSDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIACRRGQDG
                     RYQLANGMGAMLDADDALSRYEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLFAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDKPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7337..9868
                     /gene="mrcB"
                     /locus_tag="ACHLAM_000072"
     CDS             7337..9868
                     /gene="mrcB"
                     /locus_tag="ACHLAM_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO7851665.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLP
                     AAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
                     PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRS
                     GFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLF
                     LSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYF
                     GRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDM
                     LSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAE
                     KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIG
                     SLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVD
                     ALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAF
                     QTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTG
                     RQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMS
                     IYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEM
                     QQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVVGWIKDMFGSN"
     gene            10088..12277
                     /gene="fhuA"
                     /locus_tag="ACHLAM_000073"
     CDS             10088..12277
                     /gene="fhuA"
                     /locus_tag="ACHLAM_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO7851666.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12328..13125
                     /gene="fhuC"
                     /locus_tag="ACHLAM_000074"
     CDS             12328..13125
                     /gene="fhuC"
                     /locus_tag="ACHLAM_000074"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO7851667.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13125..14015
                     /gene="fhuD"
                     /locus_tag="ACHLAM_000075"
     CDS             13125..14015
                     /gene="fhuD"
                     /locus_tag="ACHLAM_000075"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO7851668.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14012..15994
                     /gene="fhuB"
                     /locus_tag="ACHLAM_000076"
     CDS             14012..15994
                     /gene="fhuB"
                     /locus_tag="ACHLAM_000076"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO7851669.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAINPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16029..17309)
                     /gene="hemL"
                     /locus_tag="ACHLAM_000077"
     CDS             complement(16029..17309)
                     /gene="hemL"
                     /locus_tag="ACHLAM_000077"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO7851670.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPHEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17475..17522
                     /gene="yadX"
                     /locus_tag="ACHLAM_000078"
     CDS             17475..17522
                     /gene="yadX"
                     /locus_tag="ACHLAM_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO7851671.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17534..18955
                     /gene="clcA"
                     /locus_tag="ACHLAM_000079"
     CDS             17534..18955
                     /gene="clcA"
                     /locus_tag="ACHLAM_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO7851672.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18979..19044
                     /gene="yadW"
                     /locus_tag="ACHLAM_000080"
     CDS             18979..19044
                     /gene="yadW"
                     /locus_tag="ACHLAM_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO7851673.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19037..19381
                     /gene="erpA"
                     /locus_tag="ACHLAM_000081"
     CDS             19037..19381
                     /gene="erpA"
                     /locus_tag="ACHLAM_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO7851674.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19428..20051)
                     /gene="yadS"
                     /locus_tag="ACHLAM_000082"
     CDS             complement(19428..20051)
                     /gene="yadS"
                     /locus_tag="ACHLAM_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO7851675.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20089..20889)
                     /gene="btuF"
                     /locus_tag="ACHLAM_000083"
     CDS             complement(20089..20889)
                     /gene="btuF"
                     /locus_tag="ACHLAM_000083"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO7851676.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20882..21580)
                     /gene="mtnN"
                     /locus_tag="ACHLAM_000084"
     CDS             complement(20882..21580)
                     /gene="mtnN"
                     /locus_tag="ACHLAM_000084"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO7851677.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21664..23181
                     /gene="dgt"
                     /locus_tag="ACHLAM_000085"
     CDS             21664..23181
                     /gene="dgt"
                     /locus_tag="ACHLAM_000085"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO7851678.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23311..24735
                     /gene="degP"
                     /locus_tag="ACHLAM_000086"
     CDS             23311..24735
                     /gene="degP"
                     /locus_tag="ACHLAM_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO7851679.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24890..26047
                     /gene="cdaR"
                     /locus_tag="ACHLAM_000087"
     CDS             24890..26047
                     /gene="cdaR"
                     /locus_tag="ACHLAM_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO7851680.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(26162..26548)
                     /gene="yaeH"
                     /locus_tag="ACHLAM_000088"
     CDS             complement(26162..26548)
                     /gene="yaeH"
                     /locus_tag="ACHLAM_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO7851681.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26706..27518)
                     /gene="yaeI"
                     /locus_tag="ACHLAM_000089"
     CDS             complement(26706..27518)
                     /gene="yaeI"
                     /locus_tag="ACHLAM_000089"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO7851682.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            complement(27571..28395)
                     /gene="dapD"
                     /locus_tag="ACHLAM_000090"
     CDS             complement(27571..28395)
                     /gene="dapD"
                     /locus_tag="ACHLAM_000090"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO7851683.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28426..31098)
                     /gene="glnD"
                     /locus_tag="ACHLAM_000091"
     CDS             complement(28426..31098)
                     /gene="glnD"
                     /locus_tag="ACHLAM_000091"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO7851684.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31160..31954)
                     /gene="map"
                     /locus_tag="ACHLAM_000092"
     CDS             complement(31160..31954)
                     /gene="map"
                     /locus_tag="ACHLAM_000092"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO7851685.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32322..33047
                     /gene="rpsB"
                     /locus_tag="ACHLAM_000093"
     CDS             32322..33047
                     /gene="rpsB"
                     /locus_tag="ACHLAM_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO7851686.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33305..34156
                     /gene="tsf"
                     /locus_tag="ACHLAM_000094"
     CDS             33305..34156
                     /gene="tsf"
                     /locus_tag="ACHLAM_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO7851687.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34303..35028
                     /gene="pyrH"
                     /locus_tag="ACHLAM_000095"
     CDS             34303..35028
                     /gene="pyrH"
                     /locus_tag="ACHLAM_000095"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO7851688.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35320..35877
                     /gene="frr"
                     /locus_tag="ACHLAM_000096"
     CDS             35320..35877
                     /gene="frr"
                     /locus_tag="ACHLAM_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO7851689.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35969..37165
                     /gene="ispC"
                     /locus_tag="ACHLAM_000097"
     CDS             35969..37165
                     /gene="ispC"
                     /locus_tag="ACHLAM_000097"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO7851690.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37354..38112
                     /gene="ispU"
                     /locus_tag="ACHLAM_000098"
                     /gene_synonym="uppS"
     CDS             37354..38112
                     /gene="ispU"
                     /locus_tag="ACHLAM_000098"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO7851691.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38125..38982
                     /gene="cdsA"
                     /locus_tag="ACHLAM_000099"
     CDS             38125..38982
                     /gene="cdsA"
                     /locus_tag="ACHLAM_000099"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7851692.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38994..40346
                     /gene="rseP"
                     /locus_tag="ACHLAM_000100"
     CDS             38994..40346
                     /gene="rseP"
                     /locus_tag="ACHLAM_000100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO7851693.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40376..42808
                     /gene="bamA"
                     /locus_tag="ACHLAM_000101"
     CDS             40376..42808
                     /gene="bamA"
                     /locus_tag="ACHLAM_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO7851694.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42930..43415
                     /gene="skp"
                     /locus_tag="ACHLAM_000102"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42930..43415
                     /gene="skp"
                     /locus_tag="ACHLAM_000102"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO7851695.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43419..44444
                     /gene="lpxD"
                     /locus_tag="ACHLAM_000103"
     CDS             43419..44444
                     /gene="lpxD"
                     /locus_tag="ACHLAM_000103"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO7851696.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44549..45004
                     /gene="fabZ"
                     /locus_tag="ACHLAM_000104"
     CDS             44549..45004
                     /gene="fabZ"
                     /locus_tag="ACHLAM_000104"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO7851697.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45008..45796
                     /gene="lpxA"
                     /locus_tag="ACHLAM_000105"
     CDS             45008..45796
                     /gene="lpxA"
                     /locus_tag="ACHLAM_000105"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO7851698.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45796..46944
                     /gene="lpxB"
                     /locus_tag="ACHLAM_000106"
     CDS             45796..46944
                     /gene="lpxB"
                     /locus_tag="ACHLAM_000106"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO7851699.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46941..47537
                     /gene="rnhB"
                     /locus_tag="ACHLAM_000107"
     CDS             46941..47537
                     /gene="rnhB"
                     /locus_tag="ACHLAM_000107"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO7851700.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRVLGLAS"
     gene            47574..51056
                     /gene="dnaE"
                     /locus_tag="ACHLAM_000108"
     CDS             47574..51056
                     /gene="dnaE"
                     /locus_tag="ACHLAM_000108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO7851701.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51069..52028
                     /gene="accA"
                     /locus_tag="ACHLAM_000109"
     CDS             51069..52028
                     /gene="accA"
                     /locus_tag="ACHLAM_000109"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO7851702.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52127..54268
                     /gene="ldcC"
                     /locus_tag="ACHLAM_000110"
     CDS             52127..54268
                     /gene="ldcC"
                     /locus_tag="ACHLAM_000110"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO7851703.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGERWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54325..54714
                     /gene="yaeR"
                     /locus_tag="ACHLAM_000111"
     CDS             54325..54714
                     /gene="yaeR"
                     /locus_tag="ACHLAM_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7851704.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54779..56077
                     /gene="tilS"
                     /locus_tag="ACHLAM_000112"
     CDS             54779..56077
                     /gene="tilS"
                     /locus_tag="ACHLAM_000112"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO7851705.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRMENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56126..56386)
                     /gene="rof"
                     /locus_tag="ACHLAM_000113"
     CDS             complement(56126..56386)
                     /gene="rof"
                     /locus_tag="ACHLAM_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO7851706.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56373..56573)
                     /gene="yaeP"
                     /locus_tag="ACHLAM_000114"
     CDS             complement(56373..56573)
                     /gene="yaeP"
                     /locus_tag="ACHLAM_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO7851707.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56739..57284
                     /gene="yaeQ"
                     /locus_tag="ACHLAM_000115"
     CDS             56739..57284
                     /gene="yaeQ"
                     /locus_tag="ACHLAM_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO7851708.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57281..57703
                     /gene="arfB"
                     /locus_tag="ACHLAM_000116"
     CDS             57281..57703
                     /gene="arfB"
                     /locus_tag="ACHLAM_000116"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO7851709.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57717..58427
                     /gene="nlpE"
                     /locus_tag="ACHLAM_000117"
                     /gene_synonym="cutF"
     CDS             57717..58427
                     /gene="nlpE"
                     /locus_tag="ACHLAM_000117"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO7851710.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58457..59281)
                     /gene="yaeF"
                     /locus_tag="ACHLAM_000118"
     CDS             complement(58457..59281)
                     /gene="yaeF"
                     /locus_tag="ACHLAM_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO7851711.1"
                     /translation="MDKPKAHCRLLLPCFLFLSACTVDISQPDSSATAVDVEAKTWVV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(59334..61052)
                     /gene="proS"
                     /locus_tag="ACHLAM_000119"
     CDS             complement(59334..61052)
                     /gene="proS"
                     /locus_tag="ACHLAM_000119"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO7851712.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61163..61870)
                     /gene="tsaA"
                     /locus_tag="ACHLAM_000120"
     CDS             complement(61163..61870)
                     /gene="tsaA"
                     /locus_tag="ACHLAM_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO7851713.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61867..62271)
                     /gene="rcsF"
                     /locus_tag="ACHLAM_000121"
     CDS             complement(61867..62271)
                     /gene="rcsF"
                     /locus_tag="ACHLAM_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO7851714.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62389..63204)
                     /gene="metQ"
                     /locus_tag="ACHLAM_000122"
     CDS             complement(62389..63204)
                     /gene="metQ"
                     /locus_tag="ACHLAM_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO7851715.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63244..63897)
                     /gene="metI"
                     /locus_tag="ACHLAM_000123"
     CDS             complement(63244..63897)
                     /gene="metI"
                     /locus_tag="ACHLAM_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO7851716.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63890..64921)
                     /gene="metN"
                     /locus_tag="ACHLAM_000124"
     CDS             complement(63890..64921)
                     /gene="metN"
                     /locus_tag="ACHLAM_000124"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO7851717.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            65109..65681
                     /gene="gmhB"
                     /locus_tag="ACHLAM_000125"
     CDS             65109..65681
                     /gene="gmhB"
                     /locus_tag="ACHLAM_000125"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO7851718.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15668 a        16312 c        18095 g        15824 t
ORIGIN      
        1 agcttcaact aatgataagc catcaggaaa tagcaattcc ggcgcaattt caaacagtgg
       61 ccacagcata aagccgcgat ttttcatatc gtagtgcgga acggtcagac gttcggtatt
      121 tattacttca ttaccaaaca gcatgatgtc gagatccagc gtgcgcggtc cccagcgttc
      181 agctttacgg acgcgacctt gctgcaattc gatacgctgt gtgtgattga gtagctcttc
      241 aggggcaaga gtggttttca gcgcgacggc tgcatttaag taatcgggtt gatcttgcgg
      301 ccccagcggt ggggtgcggt aaaacgaaga aacagcaaga atgcggcttt ctggaatatc
      361 gcctaatgct ttcagggcag cattgacctg ctccagcgga gaggccagat tgctgcctat
      421 ggcaatatac gccactgtca tgcggtaccc tcacgacgcg gtgcgcgttt gcgtggacga
      481 cgagtacggc gacgcggtga cggttcttca tccagttcgt taagcatccc tttttggtct
      541 ggtggcgcgg aaacctggaa ctcaccccac catttcacca gacgttgcag ttcagcgtta
      601 cgctcaactt cggctcgtaa ggccaacagg tcgtatgccg cacggaactt aggatgctcc
      661 agcagtttcc aggcgcgttt accctgacgt cgggacatac gcaactgcaa ctgccagata
      721 tcgcgggtta acgtcgtcag acgtttcggg atcgccagtg aacgacaggc ttcgtccagc
      781 acgtcgttca tcgccagcgc gaaagcgtcg tgataggtca ggccgctttc ctgggcgatc
      841 ttctgtgccg tctccagcag tgggtaccag aacatggcgg caaacaggaa cgccgggttc
      901 acgcgcatat cgttatggat acgcgtatcg gtattcttca gcacctgttc aatgatccgc
      961 tccatcgggc tgtcgccatt ttccgtgaag tagcgggtaa tggtcgggaa cagcggctgg
     1021 aacaggtgat attcacacag cagcttatag gtttcgtaac cgtagcccgc ttgcagcagt
     1081 ttcaaagatt cttcaaacag acgtgccggt ggaatatcgt tcagcagggt agccaggcga
     1141 gggatcggtt ctgcggtttc aggactgata cgcataccca atttggcggc aaaacgtacc
     1201 gcgcgcagca tccgtaccgg atcttcacgg tagcgcgttt ccgggttacc aatcagacgg
     1261 ataacgccgt ccttcagatc cttcatgccg ccaacgtaat cacggacggt aaaatctgcc
     1321 acgctgtaat acaggctgtt gatagtgaaa tcgcggcgct gggcgtcttc ttcgatggag
     1381 ccgaaaatgt tgtcgcgcag caacatgccg ttttgcccgc gttgggaggt cgtgcggtcg
     1441 ctgacgttac cttcgtgatg tccacggaag gtcgcaactt cgataatctc cgggccaaac
     1501 atcacatgag ccagacggaa acggcgaccc accaggcggc agttacggaa cagtttacgt
     1561 acctgttcag gcgtggcgtt agtggttacg tcaaaatctt tcggcttttt gccaagtaac
     1621 aggtcgcgca cgccaccgcc aaccagccag gcttcgtatc ccgctttatt gagcctgtac
     1681 attaccttca gggcattttc actgatatct ttgcgggaaa tagcatgctg ctcacgcggg
     1741 atcaccgtca cctgtggacg ggcgactgcc tgttcagcct cgctttcctc gcggcttagc
     1801 accttgcggc aaaaattagc gactcgggta aaaatagtac acctcggtag tgtcaaacat
     1861 cattcaggac aaaaaaatag cggctaatca tagctcagca tgacgcattt gagaatgttg
     1921 aatttacaat tgccgactca ggcacggcgg taagccgcca gtttttgacg gctgactgaa
     1981 ggatttgctc gacgctgaaa tcctgccagt gtgcttctgc ctgctgaccc agaaattgaa
     2041 gtgccgcgat tagtaccggg cgcggatcgc ctttcggcaa cgcaggcgca tgattctgct
     2101 tggaaagttt agcgccttgt gtgttaagcg ccagcggtaa atgaatgtaa tctggcactt
     2161 tccagccaaa aagctggtac agcgagattt gccgcactgt cggttcgatc aaatctgccc
     2221 cgcggacaat ttcgctcacg ccctggaagt gatcatccac caccaccgcc aggttatagg
     2281 caaacaaacc atcacggcga tgaataataa aatcttcccg cgccagtttt tcgttggcgt
     2341 gaataatgcc gcgcagctga tcagtaaata gcgtgaccgg atgctgctgg cggatacgta
     2401 ctgcggcgtt gtctggtcca tgatgcaatt cccggcaatg accgtcgtaa ataccgccaa
     2461 tgctttgaat acgcgcgcgc gtgcaggtgc agtaataact tagtccttgc tcatgtaacc
     2521 aggcgagtgc ttcgcgatag gcatcgtgac gttgcgattg ccagagaacg tcgccgtccc
     2581 agtgcagacc gtaatgttcc agctggcgca ggatagtctc tgcggcaccg ggaacttcac
     2641 gaggcgggtc gatatcttca atgcgtacca gccagcgacc ttgccgggcg cgagcctgta
     2701 aatagctgcc aagcgcggca atcagagagc caaaatgaag ctcgccggaa ggagaggggg
     2761 caaagcggcc aatatagtgt gtgtctgtca tctcgttgaa caaaaaataa ggcgggagca
     2821 tttcccgcct gtggtaaacg tgatggaacg gctgtattta gccagccatt tgtttttcgc
     2881 gaatttcagc cagcgttttg cagtcgatgc acagatcggc tgtcgggcgc gcttccagac
     2941 ggcgaatacc aatttcaaca ccgcaggatt cgcagtagcc gaaatcttcg tcttccactt
     3001 ttttcagcgt cttctcgatc tttttgatca gcttacgctc gcgatcgcgg ttacgcagtt
     3061 cgaggctgaa ctcttcttcc tgggctgcac ggtctaccgg gtccgggaag ttggctgctt
     3121 catcctgcat atgtgtaacg gtgcgatcga cttcatccct gagttgatta cgccatgctt
     3181 ccagaatacg acggaagtgc gccagctggg cttcattcat atactcttcg cccggcttct
     3241 cctgatatgg ttccacccca gcgatggcga gaatactcag ggacgatgtt ttacggtttt
     3301 gcccttcttg catgttgctt ctccttaaca cgcactatcg atccccatgt tcgggggaaa
     3361 aatgaggccg ctataaatag cagatgcttt tccggatagc aattatctaa acgtaacact
     3421 tgacaactgt gtgaggaaaa gcgtatttgc gcacgcgacc agaatgtaaa ttaaccagtt
     3481 acatacttta ctacaatgta accggcagtg attttttaag agccatgcct tcagcagaaa
     3541 gttccgcttt gtaagccaga atttctaccc ccctctgttg agcttctgac aatagttgcg
     3601 cgtatttctc atcgatgtgg cgcgcgggtg aaaaccgtgt aatggctgaa tgcagcacgg
     3661 cgaaaaagat aaccgcacgc tggccttcag ccgctacgct catcaactcc cgaaggtgtt
     3721 tctgacctcg ttcagtgacc gcatcgggaa aatatccctg ttcgttctcc gctaacgtaa
     3781 ccgatttcac ttcaatatag cagtctggac gcgaatccgc ctgcaacata aagtcaatac
     3841 ggctgcgttc ggcgccgtat tttacttcgc ttttcagcga gctatagcct gacagctctg
     3901 aaattgattc attaaggata gcctctttcg tcaacctgtt agcccaaagc gtgttgacgc
     3961 aaataaatgc gccgctctgg ctttgagtca attcccaggt gtgtgggtat ttccgtttgg
     4021 tgttgtctga agtcgaatac cagacggtat cgccaggcgt tgcacaactg gtcatcgcac
     4081 ccgtattcgg gcagtgtagc gttaattcgc gaccatcggg tgtgatcaca tcggctaaaa
     4141 aacgtttgta acgctgaatt agcgtcgcgc gctgtagagg gggagaaaat tccattacga
     4201 caatccttgt tattgcgtta gcgcccagcg ttttagcggc gtgtagcgtg tgcgtccacg
     4261 ggcaaacgag gaggcgtaaa gggtgaactc cgtcaccgta tacgaccagt taaaacctgg
     4321 cggcgggatt gtcaccgcct cgctggcgtc gcgcaataag gtaatatgtg gatgaaacgg
     4381 acgattgctt tgaaaacaac cgctgcgggc agcctgtgaa cggagcatat tcgccagctg
     4441 gattaagccg cgtggtggct gacgcatccc taaccacacc acacgcgaac gcagccattg
     4501 accggcgtca tcaagcgtga gtgtgaaacc cggttgacga atccgcccgg ctaaaagaga
     4561 aagcgccttc tctttctcgg cgctcacttc gcctaaaaat gccagagtca gatgcaaatt
     4621 atcggcggcg accggacgtc ccgcgtcagg tgggaagtgt gtggcgcgcc agtggataat
     4681 ctgttcgcgg atttctgcag gtaagtcgat agcaaagaac agccgttgcg gttcagacat
     4741 gtgaggcact cggttatgaa ttaccgcgat gctacaatgt ggcgcgaaga atgttaaccc
     4801 tctggagcgt tttgtgtcgt cgttgcccgt tgctgccgtc ttacctgagt tacttgctgc
     4861 tctcgattgt gcatcgcagg tgttattaag tgcgccgacc ggggccggga aatcaacctg
     4921 gctgccgctg caactgctgg cgcatcccgg cattaacggg aaaattatcc tgctggagcc
     4981 gcgtcgtctg gcggcgcgta acgttgcgca acgactggcg gagctgctta acgaaaagcc
     5041 aggcgatacc gttggctacc ggatgcgtgc gcaaaactgc gtcgggccga atacccgcct
     5101 ggaagtggtt accgaaggcg tgctgacgcg gatgatccag cgtgacccgg aactgagcgg
     5161 tgttggattg gtgatccttg atgaatttca tgagcgcagc ttgcaggcgg atttggcgtt
     5221 ggcgctgtta ctcgatgtgc aacaaggtct gcgtgatgac cttaaactgc tgattatgtc
     5281 ggctacgctg gacaacgacc gcttgcagca aatgctgcca gaagcgcccg tcgtcatctc
     5341 agaagggcgc tcgtttccgg ttgaacgccg ttatttgccg ctgcccgctc atcagcgttt
     5401 tgacgaagcc gtcgcggttg ccaccgccga aatgctgcgt caggaaagtg gatcattact
     5461 gttattttta cctggcgtcg gagaaattca gcgtgtgcag gaacaactgg cttcacgcat
     5521 cggcagtgat gtgttgctct gcccgctgta tggcgcgttg tcgctgaacg atcagcgaaa
     5581 agcgatcctc ccggcaccgc aagggatgcg taaagtggtg ctggcgacca atattgctga
     5641 aaccagttta accatcgaag gtattcgtct ggtggtggat tgtgcccagg agcgtgtggc
     5701 gcattttgat ccgcgtacag ggcttacgcg gctggttact caacgcgtta gtcaggcgtc
     5761 gatgacgcaa cgtgccgggc gtgccgggcg tctggagccg ggtatctgcc tgcatttaat
     5821 cgccaaagaa caagcagaac gcgctgccgc gcaaagtgaa ccggagatct tacaaagcga
     5881 tctttccggt ttgctgatgg aattactgca atggggatgc agcgatacgg cgcagatgag
     5941 ctggctggat caaccgccaa cggtgaatct acaggccgcg aaacggctgt tacaaatgct
     6001 gggggcgctg gagggtgaac ggcttagcgc acaagggcaa aaaatggcag tgctgggtag
     6061 cgatccgcgt ttagcggcaa tgctggttag cgcgaagaac gacgacgaag ctgctaccgc
     6121 ggcaaaaatt gccgccattc tcgaagagcc gccacggatg ggcaatagtg acctgggcgt
     6181 ggcgttttcg cgcaatcaac ccgcctggca gcaacgtagt cagcaactgt taaaacgctt
     6241 aaacgtacgc ggcggtgagg cagacagttc gcttatcgcg ccgctacttg ccggggcgta
     6301 tgccgatcgc attgcttgtc gccgtgggca agatggacgc tatcaactgg caaacggcat
     6361 gggggcgatg ctcgatgccg acgatgcgct aagccgctac gaatggttga tcgcaccgtt
     6421 attattgcag ggcagcgcct cgccggatgc gcggatttta ctggcgctac cggtcgatat
     6481 cgatgaatta gtacaacgct gtccgcagct ggtacagcag tcggacaccg tggaatggga
     6541 tgacgcgcaa ggcacgctga aagcctggcg tcggctgcaa atcggtcagt tgacggtgaa
     6601 agtgcagcca ctggcgaaac cctcggaaga cgagttgcat caggcgatgc ttaacggcat
     6661 ccgtgataaa ggtttaagcg tgctcaactg gacggcggaa gcggaacagc tacgcttgcg
     6721 tttgttattc gccgcaaagt ggttgccgga atatgactgg ccagcggttg atgatgagag
     6781 tttgttggcg acgctggaaa cgtggctact gccgcatatg agcggcgtgc attcactacg
     6841 cggcctgaaa tcactcgaca tttatcaggc gctacgcgga ttacttgatt ggggaatgca
     6901 gcaacgtctg gatagtgaat tgcctgcgca ttacactgtg ccgacgggaa gccggatcgc
     6961 cattcgttat catgaagata aaccgcccgc gctggcggtg agaatgcagg agatgtttgg
     7021 tgaggccacc aatccgacga tcgcccaggg gcgcgtgccg ctggtgctgg agttgctttc
     7081 ccctgcccaa aggccattac aaatcacacg agatttgagc gccttctgga aaggagcgta
     7141 ccgtgaggtg caaaaagaga tgaaagggcg ttatcccaaa catgtctggc cggacgaccc
     7201 ggcaaatact gcaccgacgc gacggacgaa aaagtattcg tgaagaccaa agcaaatttg
     7261 agagatatct tcttctgtct tgcaacagaa gaacagaaaa tcgggctttt gcgcctgaat
     7321 attgcggaga aaaagcatgg ccgggaatga ccgcgagcca attggacgca aagggaaacc
     7381 gacgcgtccg gtcaaacaaa aggtaagccg tcgtcgttac gaagatgacg attacgacga
     7441 ttatgatgac tatgaggatg aagaaccgat gccgcgcaaa ggtaagggca aaggcaaagg
     7501 gcgtaagcct cgtggcaaac gcggctggct atggctgctg ctaaaactgg ctatcgtttt
     7561 tgccgtgctg atcgccattt acggcgttta tctcgatcaa aaaattcgca gccgtattga
     7621 tggcaaggtc tggcaattgc ccgcggcagt ttatggccga atggtcaacc ttgagccaga
     7681 catgaccatc agcaagaacg aaatggtgaa gctgctggag gcgacccagt atcgtcaggt
     7741 gtcgaaaatg acccgtcctg gcgaatttac cgtgcaggcc aacagcattg agatgatccg
     7801 ccgtccgttt gatttcccgg acagtaaaga agggcaggtg cgtgcgcgtc tgacctttga
     7861 tggcgatcat ctggcgacga tcgtcaatat ggagaacaac cgtcagtttg gtttcttccg
     7921 tcttgatccg cgcctgatca ccatgatctc ctcgccaaac ggtgagcagc gtttgtttgt
     7981 gccgcgcagt ggtttcccgg atttgctggt ggatactttg ctggcgacag aagaccgcca
     8041 tttctacgag catgatggga tcagtctcta ctccatcgga cgtgcggtgc tggcaaacct
     8101 gaccgccgga cgcacggtgc agggtgcgag tacgctgacg caacagctgg tgaaaaacct
     8161 gttcctctcc agcgagcgct cttactggcg taaagcgaac gaagcttaca tggcgctgat
     8221 catggacgcg cgttacagca aagaccgtat tcttgagctg tatatgaacg aggtgtatct
     8281 cggtcagagc ggcgacaacg aaatccgcgg cttcccgctg gcgagcttgt attactttgg
     8341 tcgcccagta gaagaattga gcctcgacca gcaggcgctg ttggtgggta tggtgaaagg
     8401 ggcgtccatc tacaatccgt ggcgtaaccc gaaactggcg ttggagcgac gtaatctggt
     8461 actgcgtctg ctgcaacagc aacagattat tgatcaagaa ctctatgaca tgttgagtgc
     8521 acgtccgctg ggggttcagc cgcgcggtgg ggtgatctct ccacagccag cctttatgca
     8581 actggtgcgt caggagctgc aggcaaaact gggcgataag gtaaaagatc tctccggcgt
     8641 gaagatcttc actacctttg actcggtggc ccaggacgcg gcagaaaaag ccgccgtgga
     8701 aggcattccg gcactgaaga aacagcgtaa gttgagcgat cttgaaaccg cgattgtggt
     8761 cgtcgaccgc tttagtggtg aagttcgtgc gatggttgga ggttctgagc cgcagtttgc
     8821 gggctacaac cgtgcgatgc aggcgcgtcg ttcgattggt tcccttgcaa aaccagcgac
     8881 ttatctgacg gccttaagcc agccgaaaat ctatcgcctg aacacgtgga ttgcggatgc
     8941 gccaatcgcg ctacgtcagc cgaatggcca ggtctggtca ccgcagaacg atgaccgtcg
     9001 ttatagcgaa agcggcagag tgatgctggt agatgcgttg acccgttcga tgaacgtgcc
     9061 gacggtgaat ctgggcatgg cgttggggct gcctgcggtt acggagacct ggattaaact
     9121 gggtgtgccg aaagatcaac tgaatccagt tccagcaatg ttgttggggg cgttgaactt
     9181 aacgccaatc gaagtggcgc aggcgttcca gaccatcgcc agcggtggta accgtgctcc
     9241 gctttctgca ctgcgttcgg taatcgcgga agatggcaag gtgctgtatc agagcttccc
     9301 gcaggcggaa cgcgctgttc cggcgcaggc ggcgtatctg acactatgga ccatgcagca
     9361 ggtggtacaa cgtggtacgg ggcgtcagct tggggcgaaa tacccgaacc tgcatctggc
     9421 agggaaaaca gggactacca acaataacgt agatacctgg tttgcgggca ttgacggcag
     9481 tacggtgacc atcacctggg taggccgtga taacaaccaa ccgaccaaat tgtatggagc
     9541 cagcggggca atgtcgattt atcagcgtta tctggctaac cagacgccaa caccgctgaa
     9601 tcttgttccg ccagaagata ttgcagatat gggcgtggac tatgacggca actttgtttg
     9661 cagcggtggc atgcgtgtct tgccggtctg gaccagcgat ccgcaatcgc tgtgccagca
     9721 gagtgagatg cagcagcagc cgtcaggcaa tccgtttgat cagtcttctc agccgcagca
     9781 acagccgcaa cagcaacctg ctcagcaaga gcagaaagac agcgacggtg tagtcggttg
     9841 gatcaaggat atgtttggta gtaattaacg tctaagcgtg acataccgga tggcgagtag
     9901 ccatccggta aaataacatc ccacccaaag tattaaccct ttcttttcat ctggttgttt
     9961 attaaccctt caggaaagct cagattgcgt accgcttgcg aacccgccag cgtttcgaat
    10021 attatcttat ctttataata atcattctcg tttacgttat cattcacttt atatcagaga
    10081 tataccaatg gcgcgttcca aaactgctca gccaaaacac tcactgcgta aaatcgcagt
    10141 tgtagtagcc acagcggtta gcggcatgtc tgtttatgca caggcagcgg ttgaaccgaa
    10201 agaagacact atcaccgtta ccgctgcacc tgcgccgcaa gaaagcgcat ggggaccggc
    10261 tgcaactatt gcggcgaagc actctgctac tgcgactaaa acggatacac caattgaaaa
    10321 aacgccgcag tctatttcgg ttgtgacaaa tgaagagatg cagatgcatc aatttcagtc
    10381 tgtaaaagaa gcattaggtt atacacctgg cgttacggtt agcagtcgtg gtgcttctaa
    10441 tacatatgat tttgttatta ttcgtggttt ctcatctgtt ggtctgaatc aaaataatta
    10501 ccttgatgga ctaaaacttc agggcaactt ttataacgat gctgtgattg atccttacat
    10561 gctcgagagg gttgaactga tgcgcgggcc gacgtctgtt ctctatggga aaagtaatcc
    10621 tggtgggatt atttctatgg tgagtaagcg tccgacaact gaacccctga aagaaattca
    10681 gtttaaaatg gggacggata atctgtttca gaccggattc gatttcagtg atgcgctgga
    10741 tgataacggt gagttctcct accgtttgac tggcctcgca cgctcaacaa acgaacagca
    10801 gaaaaactct gaatctcagc gttataccat tgctccatca ttctcatggc gtccagacga
    10861 caaaaccaat tttactttcc tgtcctattt tcagaacgaa ccagaaacgg gttattacgg
    10921 ttggttgccg aaagagggga ccgttgagcc attgcctaat ggtaagcgtc taccgactga
    10981 cttcaatgaa ggtgcatcga ataatacata ctcccgtaac cagaaaatgg tgggatatag
    11041 ttttgaacat ggttttaatg acaccttcac cgtgcgtcag aatctgcgtt tcagtgaaat
    11101 gaaaacctca cagaaaagtg tttatggcac agggattgcc agcgatggtc ataccctaaa
    11161 ccgcgggaca gtggtggata atgagcgtct gcaaaacttt agcgttgata cccaacttga
    11221 aagtaaattt gctacaggtg aagtcgagca tactttgctg acaggggtag acttcatgcg
    11281 tatgcgcaat gatatcaatg ccagctttgg atctgcacca tccatcgatc tttataacaa
    11341 atatcatcct gaatactttg catttggtaa cgcagagcca taccaaatga atgaaagcaa
    11401 acaaacaggt atttatgttc aggatcaggc ggaatggaat aaatgggtat tcactctggg
    11461 gggacgctac gattggtcta agcaagcgac cactgttcgt gaaaactctt atacgccgac
    11521 tgaaggttat attgagcgta atgatcatca gttcacctgg cgcggtggtg taaactactt
    11581 attcgataat ggtatttcac cttactttag ttatagccag tcctttgaac cgagtgcttt
    11641 cgatctgtgg agcaacccgc gcgtttccta taagccatcg aaaggtgaac agtatgaagc
    11701 tggcgtaaaa tatgttccga atgatatgcc ggtcgttgtt acgggcgcag tctatcaatt
    11761 gacgaaaaca aataacctga cagcagaccc aacaaacccg ttagcgcaag tcccagcagg
    11821 tgagattcgc gctcgtggtg tggaacttga agcaaaagcg gcgttaaatg ccaatattaa
    11881 cttgacggct tcttatacct acacagatgc ggaatacacc aaagacacca atctcaaagg
    11941 taaaactcca gaacaagtac cggagcatat ggcatctctc tggggggatt ataccttcaa
    12001 tgaagggccg ctttctggtt taacattggg aacaggtggt cgttttattg gttccagcta
    12061 tggtgatccg gcgaacactt ttaaagtggg tagcgcagct gtaatggatg ctgttgtaaa
    12121 atatgatctg gcacgctttg gtatggcggg atccagcctt gctgtgaacg tcaacaattt
    12181 gctcgatcgt gagtatgttg ccagttgctt ccagacctat ggctgcttct ggggcgcaga
    12241 acgtcaggtc gttgcaaccg caaccttccg tttctaattt tttctttggg gcacggattt
    12301 ccgtgcccgc ttcacaagtt ggctgttatg caggaataca cgaatcattc cgataccact
    12361 tttgcactgc gtaatatctc ctttcgtgtg ccagggcgca cgcttttgca tccgctgtcg
    12421 ttaacctttc ctgccgggaa agtgactggt ttgattggtc acaacggttc tggtaaatcc
    12481 actctgctca aaatgcttgg ccgtcatcag ccaccgtcgg aaggcgaaat tcttcttgat
    12541 gcccagccgc tggaaagctg gagcagcaaa gcgttcgccc gcaaagtggc ttatttgccg
    12601 cagcagcttc ctccggcaga agggatgacc gtgcgtgaac tggtggcgat tggtcgttac
    12661 ccgtggcatg gcgcactggg gcgctttggt gcggcagatc gcgaaaaggt cgaagaggcg
    12721 atttcgctgg ttggcttaaa accgctggcg catcggctgg tcgatagtct ctctggcggc
    12781 gaacgtcagc gggcgtggat cgctatgctg gtggcgcagg atagccgttg tctgttgctc
    12841 gacgaaccga cctcggcgct ggatatcgcc caccaggttg atgtgctggc gctggtgcat
    12901 cgtttaagtc aggaacgtgg tctgacggtc attgccgtgc tgcacgatat taatatggct
    12961 gcgcgctact gtgattatct ggtcgccctg cgcggtggtg aaatgattgc tcagggaacg
    13021 cctgcggaaa ttatgcgcgg cgaaaccctc gaaatgattt atggcatccc gatgggtatt
    13081 ctgccgcatc cggcgggtgc tgcacctgtg agttttgttt attgatgagc ggcttacctc
    13141 ttatttcgcg ccgccgactg ttaacggcga tggcgctttc tccgttgtta tggcagatga
    13201 ataccgccca cgcggcgatt attgatccca atcgtattgt ggcgctggag tggttgccgg
    13261 tggaattact gctggcgctc ggcatcgtgc cttacggcgt ggcggacacc atcaactatc
    13321 gcctgtgggt cagcgaaccg ccattgccgg actcagtgat tgacgttggt ttgcgtacag
    13381 aacccaacct tgaactgctg accgaaatga aaccgtcgtt tatgatctgg tcggcaggat
    13441 atggcccttc accagaaatg ctggcacgta ttgcgccggg gcgcggattt aacttcagcg
    13501 acggcaaaca gccgctggcg atggcgcgta aatcactgac ggaaatggca gatttactta
    13561 acctgcaaag cgcagcagaa acgcatttag cgcaatatga agactttatc cgcagcatga
    13621 aaccccgctt tgtgaagcgt ggtgcacgcc cgttattgct gacgacgctt atcgatccgc
    13681 gccatatgtt ggtcttcggt ccaaacagct tgttccagga aattcttgat gagtacggca
    13741 tcccaaatgc ctggcaaggg gaaaccaact tctggggcag taccgccgtc agtatcgatc
    13801 gtctggcggc gtataaagac gttgatgtgc tctgttttga tcacgacaac agcaaagaca
    13861 tggatgcgct aatggcaacc ccgctgtggc aggccatgcc gtttgtccgt gcaggacgtt
    13921 ttcagcgcgt tccggcggtc tggttctatg gcgcgacgct ttcggcaatg cactttgtgc
    13981 gtattctgga taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc tttttccggc
    14041 gttattgctg gcgctgctgg tgattgtcgc tacggcgctc acctggatga acttctcgca
    14101 ggcgttgccg cgcagccagt gggcgcaggc cgcctggtcg ccggatattg acgtcatcga
    14161 gcagatgatt tttcactaca gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc
    14221 gggcctgggg ctggtgggcg tgctgtttca gcaagtgctg cgtaacccgc tggcggaacc
    14281 gacgacgttg ggcgttgcaa caggcgcgca actggggatt accgtcacta cgctctgggc
    14341 gatcccaggt gctatggcga gccagtttgc tgcgctggca ggggcttgcg ttgttggctt
    14401 aattgtcttt ggcgtcgcgt gggggaaacg gctgtcgccg gtaacgctga tcctcgcggg
    14461 gctggtagtg agcctttatt gtggggcaat caatcagtta ctggttatct tccatcacga
    14521 ccagctacaa agcatgttcc tgtggagcac cggaacgctg acgcaaaccg actggggcgg
    14581 cgttgagcgt ttatggccgc agctgctggg cggcgtgatg ctgacgttat tgctacttcg
    14641 cccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc aatctcggtc tggccttgtc
    14701 gctggcgcgt ctggcggcgc tgtcgctggc gatagtcatc agtgcgctgc tggtgaatgc
    14761 agtggggatt atcggcttta tcgggttgtt cgcgccgctg ctggcaaaaa tgctgggggc
    14821 gcggcgtctg ctgccacggt taatgctggc gtcgctgatt ggtgcgttaa ttctgtggct
    14881 ttctgatcaa atcatcctct ggctgactcg cgtgtggatg gaagtttcca ccggttcggt
    14941 cactgcgttg atcggtgcgc cgctgctact gtggctgctg ccgcgtttac gcagcattag
    15001 cgcgccggat atgaaggtca acgatcgtgt cgcggctgaa cgccaacatg tgctggcgtt
    15061 tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggta gtggcgctgt cgtttggtcg
    15121 tgatgcgcac ggctggacgt gggcgagcgg ggcgttgctc gaggatttaa tgccctggcg
    15181 ctggccgcga attatggcgg cgctgtttgc gggcgtcatg ctggcggtgg cgggctgtat
    15241 tattcagcgg ctgaccggaa acccgatggc aagcccggaa gtgctgggga ttagctccgg
    15301 cgcggcgttt ggcgtggtgt tgatgctgtt tctggtgccg ggtaatgcct ttggctggct
    15361 gttacctgca ggcagtctcg gcgcggcggt gacgctgttg atcattatga tcgccgccgg
    15421 acgcggtgga ttttccccac accgcatgtt actggcgggg atggcgttaa gtaccgcgtt
    15481 caccatgctt ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac
    15541 ctggatctcc ggttcgacct acaacgcgac cgatgcgcag gtctggcgca cgggaattgt
    15601 gatggtaatt ttgctggcga ttaacccgct gtgccgccgc tggctgacca ttttaccgct
    15661 gggcggtgat accgcacgag ccgtaggaat ggcgctgacg ccgacgcgaa ttgcgctgct
    15721 gctattagcg gcttgcctga cggcgaccgc gacgatgact attggaccgt tgagttttgt
    15781 tggtttaatg gcaccgcata ttgcgcggat gatgggtttt cgacggacga tgccacacat
    15841 cgtaatttcg gcgctggtgg gtggtttact gctggtgttc gctgactggt gtgggcggat
    15901 ggtattgttt ccattccaga tcccggcggg gctgctgtca acctttatcg gcgcgccata
    15961 ttttatctat ttgttgagaa agcagagccg ttaatagaat gcctcgcccg gtgggcgagg
    16021 caaaatcatc acaacttcgc aaacacccga cgtgcagcat cgatggtgtt attgatatct
    16081 tccatgctgt gcgccacgga cataaagccc gcttcaaacg ctgacggtgc cagataaaca
    16141 ccttcgtcca gcatcatatg gaagaaacgc ttaaagcgtt ccacgtcaca ggccatcaca
    16201 tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaaa taccgaacat gccgccaacg
    16261 tggtttacca ccagcggaat tccggcttct tctgccgctt ccagcagacc ttctgccaga
    16321 cgtgttgtca gctcatccag cgtctcgtga acgcccggct gtgcgacttc attcagacag
    16381 gcgaaaccag ctgccatcgc aatcgggtta ccggaaagcg tacccgcctg atagaccgga
    16441 ccagtcgggg ccagcgcatc cattacatca cgacgaccac cgaatgcgcc taccggcatt
    16501 ccaccgccga tgattttgcc gaggcaggtt aaatctggca ctacgccgta ataatcctgt
    16561 gcgccagcta gcgctacgcg gaaaccggtc atcacttcat cgatgatcag caacgcgcca
    16621 aattcgtcac acagcgcacg cagacctggc aggaactctg gcagcggtgg aacgcagttc
    16681 atattgcctg ccaccggctc gacgataata caggcaatct catgcgggta ctgttcgaac
    16741 gcggcgcgta cagaggccag atcgttataa gtacaggtta aggtatgttt ggcgaaatct
    16801 gccggaacac ccggcgagtt tggctggcct aacgtgagtg cgccagaacc ggctttcacc
    16861 agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg
    16921 gtaaaaccac gggccaggcg gattgcgctc atggtcgctt cagtgccgga gttcaccatg
    16981 cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt cacttccatt
    17041 tcggtcggtg caccaaagct taaaccacgc tcggcggctt caatcacggc attgcggatc
    17101 gccggatggt tatggcccag caccatcggc ccccaggaac cgacataatc gatataggct
    17161 ttgccatcaa catcgtacag ataagcgccg tccgcttttt cgataaacag tggagtgccg
    17221 cccacgccag taaaggcgcg aacaggggag ttcacaccgc cagggatcag ctcgcgcgct
    17281 gcgctgtaaa gattttcaga cttactcatg gagggttcct gattcgtaga aaaagtgaat
    17341 ggctgctatt ctatgttatt cataacaagt taaatactcg tcaaacatca ggctgcttgt
    17401 actggtgcaa tcctgatttc ttagagtata aaagttttgt gcatttgaaa cattacgctt
    17461 tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc atcttatttg tatgaccaat
    17521 aagtgatcat tggatgaaaa ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct
    17581 gcgacgcaga caactgattc gccaacttct tgagcgcgat aaaaccccgt tagccatttt
    17641 gtttatggcg gcagtcgtcg gcacgcttgt cgggctggct gcggttgctt ttgacaaagg
    17701 tgtcgcctgg ttgcagaacc aacgtatggg ggcactggta catactgctg ataattatcc
    17761 gctactgtta accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg gctacttttt
    17821 ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc ccggaaattg aaggggcgct
    17881 ggaagatcaa cgtcccgttc gctggtggcg tgtgttgccg gtgaaattct ttggcgggct
    17941 gggaacactc ggcggaggca tggtgctggg ccgcgaaggg ccaaccgtac agatcggcgg
    18001 caatatcggt cgcatggtgc ttgatgtttt ccgcctgaaa ggtgatgaag cgcgccatac
    18061 gctgctggca accggtgctg cggcggggct ggctgcggcc tttaacgccc cgctggcggg
    18121 tattttgttt attatcgaag agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa
    18181 agcggtattt attggcgtca ttatgtcgac cattatgtac cggattttta atcatgaagt
    18241 tgcgttgatt gacgtcggta aactttctga cgcgccgctt aatacgctgt ggctttatct
    18301 gatcctcggt attatttttg gcattttcgg ccctattttt aataaatggg tgctggggat
    18361 gcaggatttg ctgcaccgtg tgcacggcgg caatattacc aaatgggtac tgatgggcgg
    18421 tgcaattggc ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt cgggcggcgg
    18481 ttttaacctg attcctatcg ctaccgcggg gaatttcagc atgggaatgc tggtgtttat
    18541 cttcgtcgcg cgggtcatta ccaccttact ctgcttctct tccggcgcgc cgggcggtat
    18601 ttttgccccg atgctggcgc tgggtactgt gctgggaacc gctttcggaa tggttgccgt
    18661 tgagctgttt ccgcaatatc accttgaggc ggggacattt gctattgccg gaatgggggc
    18721 attactggcg gcatctattc gcgcgccgtt aacggggatc attctggttc tggaaatgac
    18781 cgataactac cagctcattt tgccaatgat tattaccggt cttggcgcaa cactattagc
    18841 gcaatttacc ggcgggaaac cgctatactc ggcgattctt gcgcgcacgc tggcgaaaca
    18901 ggaagctgag caactggcgc gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa
    18961 ataccagggt attagataat ggcgattatt attgggttag aatttgccca attgccgatg
    19021 tcgtttggag caaaatatga gtgatgacgt agcactgccg ctggagttta ccgacgcagc
    19081 agccaacaaa gttaaaagcc tgatcgctga cgaagataac ccgaatctga aattacgcgt
    19141 gtatattacc ggtggcggtt gcagcggctt ccagtatggt ttcacctttg atgatcaggt
    19201 gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc ctggtggttg atccgatgag
    19261 cctgcaatat ctggtcggcg gttccgttga ctataccgaa ggtctggaag gttctcgttt
    19321 catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct ttagcatcta
    19381 atctgttgtt catgtgccgg ataaactatc cggcacatct tctctcttca acgcccattc
    19441 tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa tagccgccag ccgaatcaat
    19501 agcgtcacga ccatgcccat catactggct gtttccagtg gtacggaaaa tgtgtaataa
    19561 gccgtagcgt ggacaatacc gccgataata caggctgttg cgtagatttc tgtacgtaaa
    19621 atcatgggga tctcgcgggc aagtacatca cgaatgatcc cgccgccaac gccagtaatg
    19681 acgcccatac aaaccgcgat taacggaccg gcttccgcat taaaggcttt attcacgcca
    19741 atgccgacaa acactgccag accaacggcg tccagcaccg gcaacatcca ttttggtaag
    19801 cgtcttggct ggcgcaccag cacgatggtc agcatgctgg tgaccattgc aacgaccaga
    19861 tcggtgggat ctttcaccca aaataccggg ccgtgatcca gcgccatgtc gcgaattgtt
    19921 ccgccgccta ctgcggtaac cacgcccaat accagaacac caaaagggtc catacgcaat
    19981 tttccggcta acaaaacgcc agagatagca aataccgctg tgccgactat atccagccaa
    20041 tagacgagca ttgttcaatc cccactgagc acctgaaaag gtcaggcatt aatctacctg
    20101 tgaaagcgca ttacagagct gttgtgcagc gaggataata cgtgggcttg cacgttcaaa
    20161 ccagtcactc gtgagaggaa taacgggaat tttgagctgt tcaccccagt attgtttgat
    20221 tttaggaatt tggtccggtc cgcctgtaat gacaatcgcc tgtggcgcgc gtgctaacac
    20281 ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct ttaaagatgt tttctccgcc
    20341 acaaacttcg agtacctggt tttgaatcga ctcttttcca ctagtaaatg gcggattaat
    20401 gccgaattgc agaaaaacac gttttttagg tttatcagca tattgcgctt tcaattgcgc
    20461 gtactggtcc agcagggatt gcgcggcttg ttcggcctta tccggttgcg gactccaggg
    20521 gaccagttga cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga cccacatcac
    20581 ttttattccc agagaagcca gctggtcaac ctgccgctcg gcattacctc cacgccaggc
    20641 aatcaccaga tcgggtttca gcgccacaat gcgttccaga ttcatcccct gccaggtgga
    20701 aacctgctca attttttgcg cttgtggagg atagtcggaa tagctgctga ccccaaccgg
    20761 cgtgatcccg gcggcaaagg caagttcagt gttggcggga gaaagcgtga tgacgcgcgg
    20821 cgcggcgttg agccacagcg gcgcgagaaa agacagggcg accagcgccc tgaacagtga
    20881 cttagccatg tgccagtttc tgcaccagtg actcaaccat caggctggac tgtttagcgg
    20941 caacagccag gaactcatcg aagctaagat gagactgttg atcggccacg tcggaaatgg
    21001 cgcgtacgac aacaaacggg acgttgaaat tgtggcagac atgggcgatt gccgtcgctt
    21061 ccatctctac agcaatggcc tgtgggaagt tgtggcggat tttcgccaga ccaacagaac
    21121 cattgatgaa agcgtcgccg ctgacaatca ggccacgtac agcgtttaga ttcagttcgg
    21181 caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct gccgggcagc
    21241 ctggtaactg gccgtattca taaccaaatg ccgtgacatc cgcgtcgtga taacgcgctt
    21301 cgtccgagac aacgatatcg cccactttca gtgttggtgc caggccaccg gcagaaccgg
    21361 tgttaataat cacatctggc ttgcagtgtt ccaacagcaa agtggcaccc agcgccgcag
    21421 cgactttacc gatgcccgat ttcagaagcg caacctcggt tccattcagt tggccggtat
    21481 agatttcgca accgccgaga ctgatagttt gacggttttc gattttgtca cgcagtagcg
    21541 taacttcttc ttccattgca ccaatgatgc cgattttcat agatttactc gcgataagcc
    21601 cgatttgaag gcatagttta ccatgcgctt acggggaagc gtatttctca cgcgggagag
    21661 gacatggcac agattgattt ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg
    21721 cagggcgtta aaaccgaaca tgagatccta cggatcttcg agagcgatcg cgggcgtatc
    21781 atcaactctc cggcaattcg tcgtctgcaa caaaaaaccc aggtttttcc actggagcgc
    21841 aatgccgccg tgcgcacgcg tcttacccac tcgatggaag tccagcaggt ggggcgctac
    21901 atcgccaaag aaattttaag ccgtctgaaa gagcttaaat tactggaagc atacggcctg
    21961 gatgaactga ccggcccctt tgaaagcatt gttgagatgt catgccttat gcacgatatc
    22021 ggcaatccgc cgtttggtca ttttggcgaa gcggcgataa atgactggtt tcgccagcgt
    22081 ttgcacccgg aagatgccga aagccagcct ctgactgacg atcgctgcag cgtggcagca
    22141 ctacgtttac gggacgggga agaaccgctt aacgagctgc ggcgcaaaat acgtcaggac
    22201 ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt gatgcggatg
    22261 aatctcacct gggcacaggt tggcggtatt ttaaaatata ctcgtccggc gtggtggcgt
    22321 ggtgaaacgc ctgagacaca tcactattta atgaaaaagc cgggttatta tctttctgag
    22381 gaagcctata ttgcccggtt gcgtaaagaa cttaatttgg cgctttacag tcgttttcca
    22441 ttaacgtgga ttatggaagc tgccgacgac atctcctatt gtgtggcaga ccttgaagat
    22501 gcggtagaga aaagaatatt taccgttgag cagctttatc atcatttgca cgaagcgtgg
    22561 ggccagcatg agaaaggttc gctctattcg ctggtggttg aaaatgcctg ggaaaaatca
    22621 cgctcaaata gtttaagccg cagtacggaa gatcagtttt ttatgtattt acgggtaaac
    22681 accctaaata aactggtacc ctacgcggca caacgattta ttgataatct gcctgcgatt
    22741 ttcgccggaa cgtttaatca tgcattattg gaagatgcca gcgaatgcag tgatcttcta
    22801 aagctatata aaaatgtcgc tgtaaaacat gtatttagcc atccagatgt cgagcagctt
    22861 gaattgcagg gctatcgggt cattagcgga ttattagaga tttatcgccc tttattaagc
    22921 ctgtcgttat cagactttac tgaactggta gaaaaagaac gggtgaaacg tttccctatt
    22981 gaatcgcgct tattcaacaa actctcgacg cgccatcggc tggcctatgt cgaggctgtc
    23041 agtaaattac cgtcagattc tcctgagttt ccgctatggg aatattatta ccgttgccgc
    23101 ctgctgcagg attatatcag cggtatgacc gacctctatg cgtgggatga ataccgacgt
    23161 ctgatggccg tagaacaata accaggcttt tgtaaagacg aacaataaat ttttaccttt
    23221 tgcagaaact ttagttcgga acttcgcgct ataaaacgaa tctgaagaac acagcaattt
    23281 tgcgttatct gttaatcgag actgaaatac atgaaaaaaa ccacattagc actgagtgca
    23341 ctggctctga gtttaggttt ggcgttatct ccgctctctg caacggcggc tgagacttct
    23401 tcagcaacga cagcccagca gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct
    23461 tcagtggtca gcattaacgt agaaggtagc acaaccgtta atacgccgcg tatgccgcgt
    23521 aatttccagc agttcttcgg tgatgattct ccgttctgcc aggaaggttc tccgttccag
    23581 agctctccgt tctgccaggg gggccagggc ggtaatggtg gcggccagca acagaaattc
    23641 atggcgctgg gttccggcgt catcattgat gccgataaag gctatgtcgt caccaacaac
    23701 cacgttgttg ataacgcgac ggtgattaaa gtccagctga gcgatggccg taagttcgac
    23761 gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc tgatccagat ccagaacccg
    23821 aaaaatctga ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg tgattacacc
    23881 gtagcaattg gtaacccgtt tggtctgggc gagacggtaa cttccgggat tgtctctgcg
    23941 ctggggcgta gcggcctgaa tgctgaaaac tacgaaaact tcatccagac cgatgcagcg
    24001 atcaaccgtg gtaactccgg tggtgcgttg gttaacctga acggcgaact gatcggtatc
    24061 aacaccgcga tcctcgcacc ggacggcggc aacatcggta tcggttttgc tatcccgagt
    24121 aacatggtga aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag
    24181 ctgggtatta tggggactga gctgaactcc gaactggcga aagcgatgaa agttgacgcc
    24241 cagcgcggtg ctttcgtaag ccaggttctg cctaactcct ccgctgcaaa agcgggcatt
    24301 aaagcgggtg atgtgatcac ctcactgaac ggtaagccaa tcagcagctt tgccgccctg
    24361 cgtgctcagg tgggtactat gccggtgggc agcaaactga ccctgggctt actgcgcgac
    24421 ggtaagcagg ttaacgtgaa cctggaactg cagcagagca gccagaatca ggttgattcc
    24481 agctccatct tcaacggcat tgaaggcgct gagatgagca acaaaggcaa agatcagggc
    24541 gtggtagtga acaacgtgaa aacgggcact ccggctgcgc agatcggcct gaaaaaaggt
    24601 gatgtgatta ttggcgcgaa ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt
    24661 ctcgacagca aaccgtctgt gctggcactg aacattcagc gcggcgacag caccatctac
    24721 ctgttaatgc agtaatctcc ctcaacccct tcctgaatac gggaaggggt tctccttaca
    24781 atctgtgaac ttcgccacaa ctccatacat cttcatcatc ctttgggcat ttgcacaatg
    24841 ccgtacgtta cgtacttcct tatgctaagc cgtgcataac ggaggactta tggctggctg
    24901 gcatcttgat accaaaatgg cgcaggatat cgtagcacgt accatgcgca tcatcgatac
    24961 caatatcaac gtaatggatg cccgcgggcg aattatcggc agcggcgatc gtgagcgtat
    25021 tggtgaattg cacgaaggag cattgctggt actttcacag ggacgagtcg tcgatatcga
    25081 tgacgcggtg gcacgtcatc tgcacggtgt gcggcagggg attaatctac cgttacggct
    25141 ggaaggtgaa attgtcggcg taattggcct gacaggtgaa ccagagaatc tgcgtaaata
    25201 tggcgaactg gtctgcatga cggctgaaat gatgctggaa cagtcgcggt tgatgcacct
    25261 gttggcgcag gatagccgtt tgcgggaaga actggtgatg aacctcattc aggctgagga
    25321 gaatactccc gcacttactg aatgggcgca acggctgggg atcgatctca atcaaccgcg
    25381 agtggtggct attgttgagg tcgacagcgg tcagcttggc gtggacagcg caatggcgga
    25441 gttacagcaa ctgcaaaatg cgctgactac gcccgagcgt aataatctgg tggcgattgt
    25501 ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac tcttttgggc gctgggacgc
    25561 agaagatcat cgtaagagag ttgaacaact gattacccgc atgaaagagt atggccagtt
    25621 gcgttttcgc gtttcgctgg gcaactattt taccggtcct ggcagtattg cccgatccta
    25681 tcgtacggcg aaaacgacga tggtagtggg gaaacagcgg atgccagaaa gtcgctgcta
    25741 tttttatcag gatctgatgt tacctgtgtt actcgacagt ttgcgtggcg actggcaggc
    25801 caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg gataataacg gcttgctgcg
    25861 acgtacgctg gcggcgtggt ttcgccataa cgtgcagccg ctggcaacgt caaaggcgct
    25921 gtttattcat cgtaataccc tggagtatcg gcttaatcgt atatcggaac tcacaggctt
    25981 agatctgggg aattttgatg acaggttatt gttgtatgtg gcgttacaac tggatgaaca
    26041 gcgatagggt aaaaatagta aatgccggga aatatgctcg tcttaattca agttgcatgt
    26101 acggcgactc gaattattca gcgtatactt tacccggctg gcagaatggc aatatgcaaa
    26161 attatttacg tgtcagcttt tctaaatctg cttctatctc gctgatttta ttggtcacca
    26221 ctgactccag gtgacgcaag tcgtcgagaa ttttacgctt aaggtcaact tcgctacgat
    26281 cacgctggca gatttgatca agctcatcaa tgatataacg aagattaggg ctgatttcct
    26341 gaacttcttt atatccctgg ccaataccat cggcgaccac cgttttgcgc tgacgcggat
    26401 atttaaactt cacactcttg gcgaaaaact cgcctttatc tttctgaaaa tagattttca
    26461 aaatatcgtt attggcttcc tgccggaggc tgtaacgatc aatttcctca ggattggtaa
    26521 tgcccaaact tttcagattg tcgtacatag ttgtacctca aaagagtcag taattgtgct
    26581 tatcttagca tttcgcctgg cggtttagct cgacggagat cgcaggaagc cttttcttcc
    26641 agacttttat catggcctaa aaatcaaaaa attacctgct ttattctgat aagagaattc
    26701 gctttctaca ccagttccag gattgtcact tccgggcggc aattcagacg caagccatac
    26761 aaacttccga caccccgagt cgtgtaaatt tgtcgctcac caaaggcatt taaaccggcg
    26821 gcgtagcgtt tatcttctat gggcgcaaaa ggctcaccaa ccaacggtat gcgtaactgc
    26881 ccgccgtggg tgtgaccaca gagcatcaga tcccactttt cattacgcat gacttctttg
    26941 ctgtcgggat tatgcgccag caccagtcgt ggcagatttg cttcgctggc gggaggcggt
    27001 ttgcattgtc cggtccataa atcacccgtg ccaaccagtt caaattgctg cttttgagtg
    27061 gtgattagca tggcttcgtt aaataacacc gtgatccccg ccgattttaa tgcctcgcca
    27121 attaagcgat ttttctctgt accaacaggg cgatcgtggt tgccgtaaca ggcaaatgtc
    27181 ggcgcacatt cggcaaggga ggagagtacg tcactaaacg ccgaaaaatt cagctgcata
    27241 tcaaataata cgtaatcgcc gcccagcaat atcaaatcgg gcttttgtgc tacgccaaga
    27301 gcaattgcgt cagaaatcag gcttaaagga acaaaacgag agtaatggag atctgccaga
    27361 aaaaggattt tgaaaggagc agcgttgtct ttaaaaaagg cgaggcggtg acggattaat
    27421 tcaagccagc caggttccca ataatgcata tagctgaggc ttgatccggc agctaacgtc
    27481 gcagcggctg cctgtaaaaa acgcctgcgt gtgatcatca tccatccctg caaaaaatcg
    27541 ggcagcgtca tgctgcccgt gtactgattt ttagtcgatg gtacgcagca gttcgttaat
    27601 gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataactgcac aataaaggct
    27661 gtatttgcca tctttcgacg gcaggttgcc agaaacaacc accgaaccag ccggaacgcg
    27721 accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac caatgtatac
    27781 gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt cagagcgtgc
    27841 accgatgaag caattatctt caataatggt cgggttagcc tgcagcggct ccagcacgcc
    27901 gccgatgcct acaccgccag acagatggac gtttttacca atttgtgcac aggaaccgac
    27961 agtggcccag gtatcaacca tagtgccttc atcaacatat gcgccgatgt tgacgtaaga
    28021 cggcatcagc acggtgttac gggcaataaa tgcaccctga cgtaccgccg ctggtggcac
    28081 aacgcggaag ccttctttct gaaaacgcgc ttcgtcgtag tcggcgaatt tcatcggcac
    28141 tttatcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa tacggaaaga
    28201 gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa ttttttccgc
    28261 tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt cgcgggttac
    28321 ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt caataatgtt
    28381 ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc ctttatcgtt
    28441 tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt tattaagcgc
    28501 acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa tggttgtaat
    28561 tcgggcgcca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc gcgccagcag
    28621 tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat gggtcggcaa
    28681 aaacgtcact tcggtttcaa cagtaaaatg gcgcaatttg gcgggttggc gacggggctg
    28741 cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa taacctcatg
    28801 acgatctgcg gatagcgggc taccatcggg ttccagcacg atgaaggtat ccatcgccat
    28861 accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc
    28921 ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa tctcggtgcc
    28981 tccacgcgta gcctgcggac taagcaatac cagcggtttg cttaaatcat gctgtaataa
    29041 atggcgcgca tgccaggcca gttgatttgg gctatggcgg acaaaatagt tagcacgaca
    29101 gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag
    29161 cgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg
    29221 tcgtagctgc ttttcggtgg cgaaatagag ttcgcgcaac agactttgct tccagctatt
    29281 ccacagcgtt tcgttggtgg cgcatatatc ggccactgtc aggcatacca gataacgaag
    29341 tcggttttct gtttgcactt cttcggcaaa ctgcttgatc acttccgggt cctgaatatc
    29401 gcggcgttgg gcggtcaccg acatcagcaa gtgctggcga accagccacg ccaccagctg
    29461 tgtttcgcgc gagttaagcc cgtggagttc ggcaaaatgc accacgtctt gtgcaccgag
    29521 aatggagtgg tcgccgccac gccctttggc gatatcgtga aacagcgcgg cgatgaaaat
    29581 cagctcagtt gacggcaggc gcggccagac gtctacacac aacggatggc gctggcgcgt
    29641 ttcttcactg gcaaaactct ccagtttcag catcacgcga atagtatgtt catccaccgt
    29701 gtaggcgtgg aacaggtcaa actgcatctg cccgacgata tgcgaccatt ggggcatata
    29761 cgcgccgagc acgctatgac gatgcatcgg taatagtccg cgacgcaccg caccagggtg
    29821 acgcagaatg ctcaaaaaca gttttcgcgc ttccggaata ttacacagcg gttgttgcaa
    29881 atggcgacgg gcgtggcgta actggcgcag cgtggtagag taaatgccgg tgatcgcact
    29941 gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca taaatagtgt
    30001 ttcatcacgc aggtcgatca gcgttccgcg tagctgaaac tcatcgtcga ttggacgtgg
    30061 tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca gcatctggtt
    30121 gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct cgaccggttc
    30181 gttaccttcg ccactgtaat tcagacgctg ggcgacgcta agctggcgat caaacagcag
    30241 gcgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc acaatatatg
    30301 cagacattcg tttaattccg ctcgctccgc tgaggttaag aagccaaacc cgaccatttc
    30361 atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt gaatatcgcg
    30421 caagccgcca ggactacttt tgatgtctgg ttcaaggttg tagctggtgc cgtggtaacg
    30481 ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg aaggccagaa
    30541 tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gctacatcgc caattaataa
    30601 gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca gcatgcactc
    30661 ttcaagcgtg cgcacgctat gaccgacttc cagctttaca tcccagagca gcgttaacag
    30721 ctcgcccact ttttgcgcct gatcatccgg gagcttttta cggcttaaaa tcagtaaatc
    30781 gatgtcggaa agtggatgca gctcgccacg accataacca ccgacggcaa ccaatgccag
    30841 gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagtt ggtcgataaa
    30901 ctcggtgcgt gcctcaatca actgttctgc ggagatccca ttgtcaaagg catcacccag
    30961 ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt catcacgggg
    31021 ccaggcgcat ggattttgcg gttgaccggg cagggtgggg agagcggtgt ttgcgtactg
    31081 ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat taaaaaagcc
    31141 ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg gtgtcatcct
    31201 tgcgtagcgt cagaatttcg cagccgttat cagtcaccac aatagtatgc tcatattgtg
    31261 cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg cggatctctt
    31321 ttttacccgc gttgaccatt ggctcgatgg tgaacgtcat cccaggtttc agtacgacgt
    31381 tggtttcacg ggagtcatag tgcagcacct gcggttcttc gtggaagccg cgaccaatac
    31441 cgtgtccaca atattcacga acgacggaga agccttctgc ttcgacaaat ttctgaattg
    31501 ccgcaccgat ttcgcgcaga ttaatgccag gttttaccat gcgtagcgcc aggtacaggc
    31561 tttcttgcgt gatgcggcac agacgttcgc ccatgatggt cggcttaccg acgataaaca
    31621 ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg ttaacgatat
    31681 cgccatcttt cagcagcttg gcgtcgtccg ggataccgtg gcacaccact tcattaatag
    31741 aaatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc gcgtgttgtt
    31801 cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa
    31861 catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg cgcatttttt
    31921 cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag cgtcggtgat
    31981 accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat
    32041 tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc aacgtctgat
    32101 gctgtacaca gcgccaacaa ttattggtgt ccacgacgta tttgtggtat aaagcgcgcc
    32161 ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc actttgtgta
    32221 acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta atatgggata
    32281 cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact gtttccatgc
    32341 gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg aacccgaaaa
    32401 tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt gagaaaactg
    32461 taccgatgtt caacgaagct ctggctgaac tgaacaagat tgcttctcgc aaaggtaaaa
    32521 tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct
    32581 gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac tggaaaaccg
    32641 ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac ggtactttcg
    32701 acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca
    32761 gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta atcgatgctg
    32821 accacgaaca cattgctatc aaagaagcaa acaacctggg tattccggta tttgctatcg
    32881 ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac gacgacgcaa
    32941 tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc
    33001 aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa ggcttgataa
    33061 ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc cgggagctta
    33121 catcagtaag tgaccgggat gagcgagcga agataacgca tctgcggcgc gaaatatgaa
    33181 gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg catatggccc
    33241 cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct ccgaggattt
    33301 tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta ctggcgcagg
    33361 catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc tggcaatcga
    33421 aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg ttgctgctga
    33481 cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag ttaactgcca
    33541 gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag ttctggacgc
    33601 agctgttgct ggcaaaatca ctgacgttga agtactgaaa gcacagttcg aagaagaacg
    33661 tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg ctgcgctgga
    33721 aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggtgttctgg ttgctgctaa
    33781 aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa gcaagccaga
    33841 attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc aggtacagct
    33901 ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg ttgaaggccg
    33961 catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta tggaaccaag
    34021 caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct tcatccgctt
    34081 cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag ttgctgcgat
    34141 gtccaagcag tcttaattat cgaaaaggag ccgcctgagg gcggcttctt tttgtgccca
    34201 tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac aatgtcgcta
    34261 gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac caatgcaaaa
    34321 cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg cactgaaggc
    34381 ttcggtattg atgcaagcat actggatcgc atggctcagg aaatcaaaga actggttgaa
    34441 ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg cgctggtctg
    34501 gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc gaccgtaatg
    34561 aacggcctgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg tctgatgtcc
    34621 gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat cagcctgttg
    34681 cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt taccaccgac
    34741 tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa agcaaccaaa
    34801 gttgacggcg tgtttactgc tgatccggcg aaagatccaa ccgcaaccat gtacgagcaa
    34861 ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc ggccttcacg
    34921 cttgctcgtg accataaatt accgattcgt gttttcaaca tgaacaaacc gggtgcgctg
    34981 cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt cccgtgatgg
    35041 ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt tataaggcgt
    35101 tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta caaaagtacg
    35161 tgaggatggc gagcactgcc cggggccaaa atggcaaata aaatagccta ataatccaga
    35221 cgattacccg taatatgttt aatcagggct atacttagca cacttccact gtgtgtgact
    35281 gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga tatcagaaaa
    35341 gatgctgaag tacgcatgga taaatgcgta gaagcgttca aaacccaaat cagcaaaata
    35401 cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata ttacggcacg
    35461 ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac tctgaaaatc
    35521 aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc gtccgatctt
    35581 ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc gctgacggaa
    35641 gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca
    35701 gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa agataaagag
    35761 atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac tgatgctgca
    35821 atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca gttctgattt
    35881 cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg cgttttgctt
    35941 tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg gctcgaccgg
    36001 ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact tccgcgtagt
    36061 tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg aattctctcc
    36121 ccgctatgct gtaatggatg atgaagcgag tgcgaaactt cttaaaacga tgctacagca
    36181 acagggtagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct
    36241 tgaggatgtt gatcaggtga tggcagccat tgttggcgct gctgggctgt tacctacgct
    36301 tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac tggttacctg
    36361 cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac cggtcgatag
    36421 cgaacataac gccatttttc agagtttacc gcaacctatc cagcataatc tgggatacgc
    36481 tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg gccctttccg
    36541 tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcctgcc gtcatccgaa
    36601 ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca aaggtctgga
    36661 atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag tgctgattca
    36721 cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc tggcgcagct
    36781 gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga atcgcgtgaa
    36841 ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg ccgcaccgga
    36901 ttatgatcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag gccaggcagc
    36961 gacgacagca ttgaatgccg caaacgaaat caccgtagct gcttttcttg cgcaacaaat
    37021 ccgctttacg gatatcgccg cgttgaattt atccgtactg gaaaaaatgg atatgcgcga
    37081 accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgcgtgaag tcgccagaaa
    37141 agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc gctatttgtt
    37201 agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg gctttataag
    37261 gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta ttcctgagta
    37321 ccgtaaaccc tgtcagggaa taagaaacgc gtgatgttgt ctgctactca accacttagc
    37381 gaaaaattgc cagcgcatgg ctgtcgtcat gttgcgatca ttatggacgg caatggtcgc
    37441 tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc aaaatccgtc
    37501 cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct gtatgccttt
    37561 agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga actgtttgtg
    37621 tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct gcgtattatt
    37681 ggcgatacca gtcgctttaa ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta
    37741 acagccggga ataccggtct gacgctgaat attgcggcga actacggtgg acgttgggat
    37801 atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct gcaaccagat
    37861 cagatagatg aagagatgct aaaccagcat gtctgtatgc atgaactggc ccctgtagat
    37921 ttagtaatta ggactggggg ggagcatcgc attagtaact ttttgctttg gcaaattgcc
    37981 tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca agactttgaa
    38041 ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga gcccggtgat
    38101 gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta
    38161 atacccgttg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc cattgtaacg
    38221 ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt taccactcgt
    38281 tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat gctttttctg
    38341 ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact ttgggcttcg
    38401 ctgggttggt ggattgtcgc gctattgctg gtgctgtttt accctggttc cgcagcaatc
    38461 tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt tcccttcttc
    38521 tggggcatgc tggcgttacg ggcctggcac tacgacgaga atcattacag tggcgcaata
    38581 tggctgctct atgtcatgat cctggtatgg ggcgctgact ctggcgcata tatgtttggc
    38641 aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac ctggcaaggc
    38701 tttataggtg gactcgcgac cgcagcggta atctcatggg gttatggcat gtgggcgaat
    38761 ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt agcctcagtg
    38821 ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga cagcggtcat
    38881 ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc tgcggtaccg
    38941 gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt aatatgctga
    39001 gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc accgtgcatg
    39061 aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag
    39121 ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat gttatcgccc
    39181 tgatcccgct gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg
    39241 aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg attattgccg
    39301 caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt tttattattg
    39361 gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata gctgcggaag
    39421 cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg cctgattggg
    39481 atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc attacggtag
    39541 cgccatttgg cagcgaccaa cggcgggatg taaagctcga tttacgtcac tgggcgtttg
    39601 agcctgataa agaagatccg gtaacttcgc tggggattcg tcctcgtggg ccgcaaattg
    39661 aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt ttgcaagcag
    39721 gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc
    39781 ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag gggagtccct
    39841 tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt ggttttgtcg
    39901 gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc cagtatgggc
    39961 cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag ctgacggtca
    40021 gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt gggccgatct
    40081 ctatcgccaa gggggctggg atgacagcgg aactcggggt tgtttattac ctgccgtttc
    40141 ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc gtacttgacg
    40201 gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta tccgagcggg
    40261 ttcaagactt ttgttatcgc attggctcga ttttgctggt gctgttaatg gggcttgcac
    40321 ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa taacgatggc
    40381 gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat acggtgctga
    40441 agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc
    40501 cctcctcagt atgccggtgc gcacaggcga cacggttaat gatgaagata tcagtaatac
    40561 cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga
    40621 tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt tctccggtaa
    40681 caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggcg tgcgtgtggg
    40741 cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag acttctacta
    40801 cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc cgcgcaaccg
    40861 tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc aaattaacat
    40921 tgttggtaac catgctttca ccaccgacga actgatctct catttccaac tgcgtgacga
    40981 agtgccgtgg tggaacgtgg taggcgatcg taaataccag aaacagaaac tggcgggcga
    41041 ccttgaaacc ctgcgcagtt actatctgga tcgcggttat gcccgtttca acatcgactc
    41101 tacccaggtc agtctgacgc ctgataaaaa aggtatttac gtcacggtga acatcaccga
    41161 aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg ggcactccgc
    41221 tgaaattgag cagctgacta agatcgagcc gggcgagctg tataacggca ccaaagtgac
    41281 caaaatggaa gatgacatta aaaagcttct tggtcgctat ggttatgcct atccgcgcgt
    41341 acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgtg tgaacgttga
    41401 tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga
    41461 tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggctgggga gcgatctggt
    41521 cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg ataccgatac
    41581 ccaacgcgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga aagaacgtaa
    41641 caccggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga gcttccaggc
    41701 tggtgtgcaa caggataact ggttaggtac gggttatgct gttggtatca acgggaccaa
    41761 aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca ccgtagatgg
    41821 tgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg ccgacctgtc
    41881 cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga tcaacgaata
    41941 taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca tgcagcctca
    42001 ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct ctgatcagga
    42061 taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata acaagcttga
    42121 ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag tgaccattcc
    42181 gggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg tgccgattga
    42241 tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg atggtttagg
    42301 cggcaaagag atgccgttct atgagaactt ctatgccggt ggttccagca ccgtgcgtgg
    42361 cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg ccagtaatta
    42421 tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagatc tgtgtaaatc
    42481 ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca tcaccccgac
    42541 gccgtttatt agcgataagt atgctaactc ggttcgtact tccttcttct gggatatggg
    42601 taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg actatagtga
    42661 tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc cattggggcc
    42721 gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg cagaacagtt
    42781 ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta gtggtagtgt
    42841 agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac tgcaccctcc
    42901 ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct gcaggtctcg
    42961 gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc aatatgggca
    43021 gcctgttcca gcaggtagcg cagaaaaccg gagtttctaa cacgctggaa aatgagttca
    43081 aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa atgaaaaagc
    43141 tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg atggcccagc
    43201 gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt cgttccaacg
    43261 aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt gccaacagcc
    43321 aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc gatgtaaaag
    43381 acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt cgactggctg
    43441 atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc atcaccggcg
    43501 ttgcgtccat gcaatctgca caaacaggtc acatcacgtt catggttaac ccaaaatacc
    43561 gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac gatcttcctt
    43621 tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg cgcatggcgc
    43681 aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg gtgattgacg
    43741 cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt gagtccggcg
    43801 ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa aacagcaaaa
    43861 tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc cagatcggtc
    43921 agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt tatgccaacg
    43981 atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc gatcgcgtgg
    44041 agatcggtgc ctgcactacc attgatcgtg gcgcgctgga tgacactgtt attggcaatg
    44101 gtgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc gacaatacgg
    44161 cagttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac tgcatgatcg
    44221 gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg gttacgggca
    44281 tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc attccgctgc
    44341 aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat gacatgagca
    44401 agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc atcttttgtt
    44461 cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt attattgtcg
    44521 tttcttatat tttgacagga agagtatctt gactactaac actcatactc tgcagattga
    44581 agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc gcgtgctgga
    44641 ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg agccattctt
    44701 ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg aagcaatggc
    44761 acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg gtgagctgta
    44821 ctacttcgcc ggtattgacg aagcgcgctt taagcgcccg gtcgtgcctg gcgatcaaat
    44881 gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta aaggggttgc
    44941 tctggtcgac ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta gccgggaggc
    45001 ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg aagagggcgc
    45061 gtcgattggc gcgaacgctc acattggtcc tttttgtatc gttggacccc atgtcgaaat
    45121 tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta aaattggccg
    45181 cgacaatgag atttatcagt tcgcctccat cggcgaagtc aaccaggatc ttaaatatgc
    45241 tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa gcgtcaccat
    45301 tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca acttactgat
    45361 gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc tcgccaacaa
    45421 cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg gcatgaccgc
    45481 agtccatcag ttctgcatca ttggtgcgca cgtgatggtt ggcggctgct ccggtgtggc
    45541 gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt tcggtgtcaa
    45601 tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta tccgcaatgc
    45661 gtataagctg atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg aaattgctga
    45721 actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac gctcaacgcg
    45781 cggtctgatt cgttaatgac tgaacagcgt ccattaacga ttgccctggt cgccggagaa
    45841 acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg tgtgcccaac
    45901 gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga agcctggtac
    45961 gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct gcgtcgctta
    46021 ctgcatattc gtgccgatct gacaacgcgt tttggcgaac tgaagccaga tgtttttgtc
    46081 ggtattgatg cgcctgactt caatattacc cttgaaggta accttaaaaa gcagggtatc
    46141 aaaaccattc attatgtcag tccgtccgtc tgggcgtggc gacagaaacg cgttttcaaa
    46201 ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc gttttatgac
    46261 aaatataacg tgccgtgccg ttttatcggt cacaccatgg ctgatgccat gccgttagat
    46321 ccagataaaa acgccgcgcg tgatgtgctg gggatccctc acgatgccca ctgtctggca
    46381 ttgttgccgg gaagccgtgg cgcggaagtg gagatgctta gcgccgattt cctgaaaacg
    46441 gcccagcttt tgcgccagac gtatcctgat ctggagatcg tggtgccgct ggtaaatgcc
    46501 aaacgccgtg agcaatttga acgcatcaaa gctgaagtcg cgccagacct ttcggttcat
    46561 atgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcgct attggcgtcg
    46621 ggtacggctg cactggagtg catgctggcg aaatgcccga tggtggtggg gtatcgcatg
    46681 aagcctttca ccttctggtt ggcgaagcgg ctggtgaaaa ccgattatgt ctcgctgcca
    46741 aacctgctgg cgggcagaga gttagttaaa gaattattgc aggaagagtg tgagccgcaa
    46801 aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca cgcgatgcac
    46861 gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca ggcggcacaa
    46921 gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcac ctggttgcgg
    46981 gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct gcggtgatcc
    47041 tcgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc gaaaaacgcc
    47101 gtctggcgct ctgcgaagag atcaaagaga aagcgttgag ctggagtctg ggccgagcgg
    47161 aacctcacga aatcgacgaa ctgaatattt tacacgcgac catgctggcg atgcagcgtg
    47221 ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac cgctgcccga
    47281 aattaccgat gcctgcgatg gctgtggtga aaggcgatag ccgcgtaccg gaaatcagtg
    47341 ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg gatattgttt
    47401 tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat ctggaaaaac
    47461 tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc aaacgcgtac
    47521 tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg aagatgtctg
    47581 aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat ggtctggcca
    47641 aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg gcgatcaccg
    47701 atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc gcagggatta
    47761 agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat gagttaaccc
    47821 acctgacggt gctggcggcg aacaataccg gctatcagaa tctgacgttg ctgatctcaa
    47881 aagcgtatca gcgcgggtac ggtgcagctg ggccgatcat cgatcgcgac tggcttatcg
    47941 aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc ggacgcagtc
    48001 ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc
    48061 cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggacgaagaa agctatctgc
    48121 acgctgcggt ggaactggcg gaagcacgcg gtttgcccgt tgtggcgacc aacgacgtgc
    48181 gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc cacgacggct
    48241 ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat atgcgtagcg
    48301 aagaggagat gtgcgagctt tttgccgaca ttcccgaagc ccttgccaac accgttgaga
    48361 tcgccaaacg ttgtaacgta accgtgcgtc ttggtgaata cttcctgccg cagttcccga
    48421 ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagagggg ctggaagagc
    48481 gtctggcctt tttattccct gacgaggaag aacgtgttaa gcgccgcccg gaatatgacg
    48541 aacgtctgga gactgaactt caggttatca accagatggg cttcccgggt tacttcctca
    48601 tcgttatgga atttatccag tggtcgaaag ataacggcgt accagtaggg ccaggccgtg
    48661 gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc gatccgctgg
    48721 aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg cctgacttcg
    48781 acgttgactt ctgtatggag aaacgcgacc aggttatcga gcacgtagcg gacatgtacg
    48841 gtcgtgatgc ggtatcgcag atcatcacct tcggtacgat ggcggcgaaa gcggtgatcc
    48901 gcgacgtagg tcgcgtgctg gggcatccgt acggctttgt cgatcgtatc tcgaaactga
    48961 tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg cagctgccgg
    49021 aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc aaactggaag
    49081 gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg accaaaatta
    49141 ccgattttgc gccgctttac tgcgatgaag agggcaaaca tccggtcacc cagtttgata
    49201 aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg cgtacgctca
    49261 ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat ggcgagccgc
    49321 cgctggatat cgccgcgatc ccgctggatg ataagaaaag cttcgacatg ctgcaacgct
    49381 cggaaaccac tgcggtattc cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc
    49441 tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc cccggtccgt
    49501 tgcaatcagg gatggtggat aactttatcg accgtaaaca tggtcgcgaa gagatctcct
    49561 atccggacgt gcagtggcag catgaaagcc tgaaaccggt actggagcca acctacggca
    49621 tcatcctgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt tataccctcg
    49681 gtggcgcaga tatgctgcgt cgtgcgatgg gtaagaaaaa gccggaagag atggctaagc
    49741 agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa ctggcgatga
    49801 aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg cactctgcgg
    49861 cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg gcagagttta
    49921 tggcggcggt aatgaccgcc gatatggaca ataccgagaa ggtggtgggc ctggtggatg
    49981 agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt ctttaccatt
    50041 tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg cgcgatcaaa ggggtaggtg
    50101 aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc cgcgaactgt
    50161 ttgatctctg cgcccgaacc gacaccaaaa agttaaaccg gcgggtgctg gaaaaactga
    50221 tcatgtccgg ggcgtttgac cgtcttgggc cacaccgtgc ggcgctgatg aactcgctgg
    50281 gcgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt caggccgata
    50341 tgttcggcgt gctggccgaa gaaccggaac aaattgaaca atcctacgcc agctgccaac
    50401 cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggtctg tacctgacgg
    50461 gacaccctat caaccagtat ctaaaagaga ttgagcgtta tgtcggaggc gtaaggctga
    50521 aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc gttgttgccg
    50581 cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt
    50641 ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag caattgctgg
    50701 aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc agcggtgggc
    50761 ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg
    50821 ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac cgactccgtc
    50881 agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat cagagggcgg
    50941 atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat
    51001 taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt gactaataca
    51061 ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag ctggaagcga
    51121 aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt aacatcgatg
    51181 aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc ttcgccgatc
    51241 tcggagcatg gcagattgcg caactggcac gccatccaca gcgtccttat accctggatt
    51301 acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgcgcg tatgcagacg
    51361 ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg atcattggtc
    51421 atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg ccagcgccag
    51481 aaggttaccg taaagcactg cgtctgatgc aaatggctga acgctttaag atgcctatca
    51541 tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag cgtggtcagt
    51601 ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct cggcgtaccg gtagtttgta
    51661 cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc gataaagtga
    51721 atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg tccattctgt
    51781 ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc gctccgcgtc
    51841 tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc gctcatcgta
    51901 acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg gccgatctcg
    51961 acgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg agctacggtt
    52021 acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt ttatcgccac
    52081 ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga acatcattgc
    52141 cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac tggagtcggc
    52201 gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt tgctgaaatt
    52261 tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt acagtctcga
    52321 tttatgtagc gatatcaatc agcttaatga atatctcccg ctttatgcct tcatcaacac
    52381 ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt tttttgaata
    52441 tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacaccg acgaatatct
    52501 tgataacatt acaccgccgt tcacgaaagc cttgtttacc tacgtcaaag agcggaagta
    52561 caccttttgt acgccgggtc atatgggcgg caccgcatat caaaaaagcc cggttggctg
    52621 tctgttttat gattttttcg gcgggaatac tcttaaggct gatgtctcta tttcggttac
    52681 cgagcttggt tcgttgctcg accacaccgg gccacacctg gaagcggaag agtacatcgc
    52741 gcggactttt ggcgcggaac agagttatat cgttaccaac ggaacatcga catcgaacaa
    52801 aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttaattgacc gcaattgtca
    52861 taaatcgctg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac
    52921 gcgtaatgcg ttggggattc ttggtgggat cccgcgccgt gaatttactc gcgacagcat
    52981 cgaagagaaa gtcgctgcta ccacgcaagc acaatggccg gttcatgcgg tgattaccaa
    53041 ctccacctat gatggcttgc tctacaacac cgactggatc aaacagacgc tggatgtccc
    53101 gtcgattcac ttcgattctg cctgggtgcc gtacacccat tttcatccga tctaccaggg
    53161 taaaagtggt atgagcggcg agcgtgttgc gggaaaagtg atcttcgaaa cgcaatcgac
    53221 ccacaaaatg ctggcggcgt tatcgcaggc ttcgctgatc cacattaaag gcgagtatga
    53281 cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca gttatcccat
    53341 tgttgcttcg gttgagacgg cagcggcgat gctgcgtggt aatccgggca aacggctgat
    53401 taaccgttca gtagaacgag cgctgcattt tcgcaaagag gtccagcggc tgcgggaaga
    53461 gtctgacggc tggtttttcg atatctggca accgccgcag gtggatgaag ccgaatgctg
    53521 gcccgttgcg ccaggcgaac gatggcacgg ctttaacgat gcggatgccg atcatatgtt
    53581 tctcgatccg gttaaagtca ctattctgac accggggatg gacgagcagg gcaatatgag
    53641 cgaggagggg atcccggcgg cgctggtggc aaaattcctc gacgaacgtg ggatcgtagt
    53701 agagaaaacc ggcccgtata acctgctgtt tctttttagt attggcatcg ataaaaccaa
    53761 ggcaatggga ttattgcgtg ggttgacgga attcaaacgc tcttacgatc tcaacctgcg
    53821 gatcaaaaat atgctacccg atctctatgc tgaagatccc gatttctacc gcaatatgcg
    53881 tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc tttccggttt
    53941 gatgttgcgg gcatttgata ccttgccgga gatgatcatg acgccacatc aggcatggca
    54001 gagacagatt aaaggcgaag tggaaaccat tgcgctggaa caactggtcg gtagagtatc
    54061 ggcaaatatg atcctgcctt atccacctgg cgtaccgctg ctgatgccag gagaaatgct
    54121 gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg tagggcaaca
    54181 ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg gcgtttaccg
    54241 cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc gagtaacgtg
    54301 ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca tattgcgatt
    54361 attgcgacgg attatgcggt gagcaaagcg ttttactgcg atattcttgg tttcacgctg
    54421 caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc gcttaatggg
    54481 caatatgtga ttgagctttt ctcatttccg ttcccgccgg agcgccccag ccgaccggaa
    54541 gcttgcggcc tgcgtcatct ggcttttagc gttgatgata tcgatgcggc agtggcgcac
    54601 cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc
    54661 ttcacgttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca gtaaggcttg
    54721 tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc gcatcatcat
    54781 gacactcacg ctcaatagac aacttctctc ctcacgccag attctggtgg cctttagcgg
    54841 cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggatgg aaaatccggg
    54901 cgtcactctg cgcgctatcc atgtgcatca cggtttaagt gctaatgccg atgcctgggt
    54961 taggcattgc gagaacatct gccaacagtg gcaggtgccg ctggtggtcg agcgcgtgca
    55021 acttgcgcaa gaaggactgg gcattgaggc ccaggcgcgg caggcacgtt atcaggcatt
    55081 tgcccgcacc ttgttacccg gtgaagtgct ggtcaccgcg caacatctcg acgatcaatg
    55141 tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt cggctatggg
    55201 ggaagtttcg gagtttgccg gaacacggct tatccgcccg ttgctcgccc gcacgcgggg
    55261 agaactggtg cagtgggcgc gtcagtatga tttacgctgg attgaagacg aaagtaatca
    55321 ggatgatagc tacgatcgta acttcctgcg cctgcgcgtg gtgccgttat tgcagcagcg
    55381 ttggccgcat tttgccgaag caacggcccg cagcgctgcg ctttgcgctg aacaagagag
    55441 cctgttggat gaactgctgg cggatgatat agcacactgt caaacgtcgc aggggacgct
    55501 gcagattgcg ccaatgctgg cgatgagtga tgcccgccgc gcggcgatta tccgccgctg
    55561 gctggcaggg aagaatgcac cgatgccttc ccgcgacgcg ttggtgagga tctggcagga
    55621 agtggcgctg gcgcgggaag atgcttcccc ctgtttacgc ctgggcgcgt ttgaaatccg
    55681 ccgctatcag tcgcaactgt ggtggattaa atccgtcact gggcaaagcg aaaccattgt
    55741 gccgtggcag acgtggctac aaccgctgga attaccggcg ggactgggaa gtgtacagct
    55801 tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa
    55861 agcgccagga ttgctgcata ttatcgggcg caacggcgga cgtaagctga agaaaatctg
    55921 gcaagagctg ggtgtgccgc cgtggctacg tgacaccacg ccactgctgt tttatggcga
    55981 aacgttgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg aaggtgagaa
    56041 tggcgtcagt tttgtctggc agaaaacgct tagttaagtg aaagccggat aagacgcttc
    56101 aaacgtcgca tccggcgaaa gtcaatcagg actcgctcac caccaccgta ccgatttccg
    56161 ggtggctaaa gctggtaatt ttatccagac gcagctcgcg ggttgtacca gcggcctcga
    56221 cgaccagata ctccacattt ttgcgggaga ctaaatcgct ggccttcgcc tgcaattttt
    56281 cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc tcaagattat
    56341 cgtaatcatc acaattgatt ggttgatacg tatcattcat tgacataatc gctcaccagt
    56401 aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc gtcgtcggca
    56461 gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta tcctaagtct
    56521 ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc cacatgccct
    56581 cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga taaactctgt
    56641 ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac tgcacgtatt
    56701 ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt
    56761 ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat gcctctctga
    56821 cgctggcgcg ccacccttca gaaacccagg agcgcatgat gctgcgcctg ctggcgtggc
    56881 taaaatatgc cgatgaacgt ctgcaattta cccgtggttt gtgtgccgat gatgagccgg
    56941 aagcgtggct gcgtaacgat catttgggta ttgatttgtg gattgagctg gggctgccgg
    57001 atgagcgacg gattaagaaa gcctgcaccc aggccgcaga agtggcgctg tttgcctata
    57061 atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgcgcg cagttttcca
    57121 atctttccat ctggtatctg gacgatgaac aactggcgaa agtgagcgcc tttgccgatc
    57181 gtaccatgac gctgcaggca acgattcagg atggcgtgat ctggttatcg gatgataaga
    57241 ataatctgga agtgaactta accgcctggc aacaaccttc atgattgtga tttcccgaca
    57301 tgttgctatc cccgatggtg agcttgagat caccgccatt cgtgcgcagg gcgcgggcgg
    57361 gcagcatgtt aataagacct caacggctat tcatctgcgt tttgacattc gggcgtccag
    57421 cctgccagag tattacaaag aacgtctgct cgccgccagc catcacttga tcagcagtga
    57481 tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc gcgaagcggc
    57541 tctggcccgg ctggtggcag tgattaaaga tttaacaaca gaacaaaaag cccgacgacc
    57601 cacgcgcccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac aaaaatcaag
    57661 cgtgaaggcg atgcgcggca aagtgcgcag cggtcgggaa taaaaagaag gaatggatgg
    57721 tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg atgggttgta
    57781 ataatcgggc cgaagtcgat acgctttctc cggcgcaggc tgccgaactg aaaccgatgc
    57841 cgcaaagttg gcgcggcgtg ctgccgtgtg ctgattgcga aggaatcgaa acctctctgt
    57901 tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct cgtgaagaac
    57961 cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagcta gtattaaccg
    58021 acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag atgctcgatc
    58081 gtgaaggcaa tccgattgaa tcgcagttca actatacgct agaaccggca caatccagtt
    58141 tacctatgac gccgatgacc ctgcggggca tgtattttta tatggctgat gcggcgacct
    58201 tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag ctggagcgta
    58261 gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt attgtcagta gaaggtcact
    58321 ttacgcttga gggtaatccg gataccggtg cgccgactaa agtattggca cccgatacgg
    58381 cgggtaaatt ttatccgaac caggattgca gtagtttggg gcagtaattg aaagccggag
    58441 cattctccgg cttttatcac tgagtcagcc caacaaagcg acccgccttg atataaatcc
    58501 ctggcttcat gtgcccaaca tactgtaact gcgtttctgg cttaaacgca gagatatcgc
    58561 cagtacgcat atgcatcaga tcggcagggc taatccagcc tgattgcgcc agcgtaagcg
    58621 gatgcccggc tttggcaaat gcatcagtga caaattccga acaaaaccac gactttttgt
    58681 ctccttcgcc cacactgctt aactgcgctt tcgccaggcc gctgacgcac tgttgacgaa
    58741 aatcctcgga gaacggattc agtgagcaca cctggcgagt caccataaag ggaataaatt
    58801 cgacaatgcc gcgatagtta taaccgctat ctttgatttt attagcaaac gcggtgatat
    58861 ctgtggcttg ttgcggggta agatccggga ctcgtaagac gaaaagctta tcactgtgct
    58921 tcatcgcttt tttaagggaa acgatctgga cgccagcgcc tgtcgcttct gcaacgttat
    58981 tatcacccag aaagattgca acgtgactca cagaggaagt gctgaagacg cggattccaa
    59041 atgaggttac cccaaggctt gaggagaaca gcaaatcgcc gggtttgaga tcaggtgccg
    59101 ttatttcttt tattgattgt tcggtgaaag agctttgatg ctggaatttg actacccatg
    59161 tttttgcctc gacatctacc gctgtggcag aggagtctgg ctggctgata tcaacggtac
    59221 aggccgaaag gaaaagaaag caggggagaa gcaggcggca atgcgccttt ggtttatcca
    59281 ttttatacaa tccatgtaaa aaagggccct gaaattcagg accctttctg gcatcagcct
    59341 ttaatctgtt tcaccagata ttcgacgatg tcaccagtct taattaactg tttctcgccg
    59401 ttacgacgat atttatattc gatatcatcg ttgtcgaggt tacggtcgcc cagcacaata
    59461 gtgtgcggaa taccgatcag ttccatatca gcaaacatca cgcccggacg ctctttgcgg
    59521 tcatccagca gcacttcgat accttgtgca cgcagttcgc tgtacagttt ctcagcaagc
    59581 tcttgtacgc ggaaggattt gtgcatgttc atcggcagaa tcgccacctg gaaaggcgcg
    59641 atagcgtcag gccatacgat gccgcgttcg tcatagttct gctcaattgc cgcagccaca
    59701 acgcgcgtta ccccgatacc gtagcaaccc atcgtcagga tttgattacg gccatcttca
    59761 ccctgtacgg aggctttcag tgcttcggag tacttggtac ccagctggaa gatgtgacca
    59821 acttcgatac cacgtttgat cagcagcgta ccctgaccat ccgggcttgg atcgccagcc
    59881 accacgttgc ggatatcagc aatttccggg gtagcgacat cgcgatccca gttgataccg
    59941 aagtagtgtt taccatcgat gttagcacca gcagcgaaat cactcatcgc cgcaacggta
    60001 cggtcaatca ccaccggaat tggcatgttt accggaccca gtgagccagg gccggcttta
    60061 accacggcac gaatttcttc ttcggtcgcg aaagtcagcg ggcttgcaac ctgcggcagt
    60121 ttttctgctt taacttcgtt cagctcgtgg tcaccgcgca ccagcagcgc aaccagcggg
    60181 aagctactgc cttcaaccgc tttaaccagc agggtcttaa ccgttttctc aatcggcaga
    60241 ttgaactgtt caaccagttc cgcgatggtc ttcgcgttcg gcgtatcaac cagcgtcatt
    60301 tcctgggtag cagcagcgcg cggttctttc ggcgcgatag cttctgccag ttcaatgttc
    60361 gctgcatagt cagaggtgtc ggagaagacc acatcgtctt caccgctttg cgccagcacc
    60421 tggaattcgt gagaggcgct accgccgata gaaccggtat cggcctgtac ggcgcggaaa
    60481 tccagcccca tgcggctgaa gattttgctg taggccgcat acattgcatc gtaggtttcc
    60541 tgcagggatt cctgagaggt atggaaagag taagcatctt tcatcaggaa ttcgcgggaa
    60601 cgcatgacgc cgaaacgcgg gcgcacttca tcacggaact tagtctggat ctgatagaag
    60661 ttcagcggca gctgtttgta agagctcagc tcgttacgaa tcaggtcggt aatcacttct
    60721 tcatgagttg ggccaagtac gaacggacgg tcaccacggt cagcaatacg cagcaattcc
    60781 gggccatact gttcccaacg accactctct tgccacagtt cagatggctg aaccaccggc
    60841 attaacacct caatcgcacc ggcgttgttc atctcttcac gcacgatgtt ttcgactttt
    60901 ttcagaacgc gcacgccggt cggcagccag gtgtataacc cggaggccag cttgcggatc
    60961 atcccggcgc gcagcatcag ctgatggctg atcacctcgg cgtcggcagg tgtctccttg
    61021 agagtggaga gcaggtattg gctagtacgc atgttgttac ggttccagtt ggaaggtaga
    61081 acaggctcaa ggtgagcctg ggacaaaaaa agtgatttag tttaccagtg caaaagaaat
    61141 gtcaaaagag aagggcgtga atttaacgcg gttccagcgc aaagacttca aaacctgcgt
    61201 cggtgacgcg ccagcgaacg ttaaaatcat gcagccagac ggcataggtt ttgcccgttt
    61261 cctcaccttt acgataggcc gggcgcgggt cctgcgctag tacttcgcgg ataaacagcg
    61321 ttaactgcgg ataacgcttc tccagcgtca aaagctgctt ttcgacctct gcggtaaaac
    61381 tcaccgccat ctctgcagct ggcgcacttt gcgcatagct ggcactggca tttggaagcg
    61441 attcggcaaa ggggagatac ggtttgatat ccactaccgg cgtaccatcg accagatcca
    61501 gactgccgag cttcagaatc acgctgtctt tatggcaaac aacctctttc agctctacca
    61561 gcgacatacc aattgggtta gggcgaaaag tggagcgtgt tgcgaaaacc cccattctgg
    61621 cattaccgcc aagacgaggc ggacgcacag tcggacgcca gccgccttcc atcgtttgat
    61681 gaaagacgaa aagtatccat aaatggctga acgcttccag gccgcgaacg gcgtcggctt
    61741 ggttgtaggg agcaatgaga tgcagttcgc cgttggcgct ttttaccaga cctggttggc
    61801 gcggaacggc gaatttttct ttatagggcg agcgaataac gcctatttgc tcgaactgga
    61861 aactgctcat ttcgccgtaa tgttaagcgc agaaccgata catacagcct gacgatagca
    61921 gcctggcgta ccgctggtga cttcgcagct atgcagtaat accgcattgg ctttcatttt
    61981 cgaggcgttg atttgcatcc gcttacgcgc ggttggaatg ctcggcggag agtcctgatt
    62041 agaggcttgg caagagtcgc cactgacttc accgagatcg cggaacggtt tgccgactaa
    62101 ttcttctgca ttggtataaa ttcggaccgg cgtggcgcgc ggcactttcg gttttgcaga
    62161 ctccgctttc ggctggggtg cagtgctttg aacgggttcg acaggggatc tgcttaacat
    62221 ggaacagccg cttagcatga gtgctactaa acagatcggt aaagcacgca tagtatttcc
    62281 tcaatgtatg atcaaaacgt caatattgaa tcaggagctt gtaaaaatga caagacgggc
    62341 aagcgcccgt cctgaatgat attacaaatt gtggaaacag cctaaaaatt accagccttt
    62401 aacagcgccg ccgttaaaca ctttgtttgc tgcttcgtaa acttcgtcag actgataagc
    62461 ctggacgaat ttcttcacgt tctcggcgtc tttgttatct tcacgcgtta cgatcaggtt
    62521 tacgtacggg gactctttat cttcgacaaa gataccgtct ttcgccggag tcagaccaat
    62581 ctggctggca taggtggtat tgataactgc cagagcgatt tgcgcgtcgt ccagagaacg
    62641 cggcagctgc ggtgcttcca gctcaacaat tttcagattt ttggggttct caacaacatc
    62701 aagaacggtc ggcagcaggc caacgccatc tttcagtttg atcaagccca ctttttgcag
    62761 cagcagcagt gaacgaccaa ggttagttgg gtcgtttggc acggcaacct gcgaaccatc
    62821 ctgcagttca tccagtgatt tgattttctt ggagtaacct gcaatcggat aaacaaaagt
    62881 gttgcctact gcgaccagtt tgtagccacg atctttcagt tgctgatcaa ggtacggttt
    62941 atgctggaag gcgttggcgt cgatatcgcc tttgctcaat gcttcgtttg gcagaacata
    63001 gtcgttgaat gttaccagct caacgtccag gccatatttg tctttcgcaa ctttctgcgc
    63061 gacttctgca acctgctgtt cggcaccaac aatcacgccg actttaatgt ggtttggatc
    63121 tttttcatcc tgaccgcagc ctaccagtgc cagtgatcca atcagggctc ccactgccgc
    63181 aaaggttttg aatttgaacg ccatacctta ttccttcttc aattatttat gttgtgttga
    63241 acgttacttg cgagtgacag cccggacgat gcggtcgcct gcgaactgaa ttaaataaac
    63301 cagaatgacc agcaatacca gtaccgtatt catcaccgtc gcgttgtagc cgatgtagcc
    63361 atactgatag ccaatctgac ctaaaccacc ggcaccgact gcaccaccca tcgcggaata
    63421 accgaccagg gtaatcaggg tgatagttgc cgcatttacc agacccggca acgcttccgg
    63481 taacagcact ttacggacga tctgcattgg cgtggcaccc attgcgcggg aagcttcaat
    63541 taacccggtt gggatctcca gcagagcgtt ctcgaccata cgggcaataa acggtgctgc
    63601 accaacggtt aacggaacaa tcgctgcctg caaaccaatc gatgtaccaa caataacgcg
    63661 ggtaaacgga atcatccata ccagcaagat aatgaacggg atggaacgga aaatgttcac
    63721 aatcgcagaa acggtacgat acagcttcgc gttagcaata atttgccccg gacgcgtgac
    63781 ataaagcagg acgccaaccg gcagaccaat cacaaatcca aaaaaaccgg atacgaaggt
    63841 cattgccagc gtttcccata cgccacgaac cagcagccac atcatcggct cagacataac
    63901 ccagtacctc tacttttaca tggtgttcct gcagccaggt aatggcggct tgcgtatctt
    63961 gttgtgtgcc gtgcatttca gtcagcatga tgccgaactt aacgccaccg gcgtaatcca
    64021 tctgcgcgct aataatgttg ttgttgacgt tgaaacgacg cgcggtttca gaaagcagtg
    64081 gggcatcgac cgattgaccg gtaaactcca gacgcaacat cggcacgcag tcagtgaatg
    64141 gctccgcttg cagacgttcc tggtaatctt ccgggatatc cagatgcagg gtcgactgaa
    64201 taaacttctg cgccagcggc gttttcggat gcgagaacac ttcacttacc gtgtcctgct
    64261 cgatcagttc gccattgctg atgaccgcca cgcaatcaca aatacgcttc acaacgtcca
    64321 tttcgtgggt gatcaacaga atcgtcaaac ccagacggcg gttgatgtct ttcagcagtt
    64381 cgagaataga acgtgtcgtt gccggatcca gcgcgctggt ggcctcatca cacagcaata
    64441 ctttgggatt gctggctaac gcacgggcaa tcgccacacg ctgtttctgc ccaccggaaa
    64501 gattcgacgg gtagctatca tgcttatcgc caagaccaac caatgacagc aattccgtca
    64561 cgcgacgttt gatctcatct ttcggtgtgt tgtccagctc cagcggcaga gccacgttgc
    64621 caaaaacagt acgcgaagag agcaggttaa aatgctggaa aatcatacca atctggcggc
    64681 gagctttggt caactcggat tctgacagcg tggtcagttc ctggccatcg accagcacgc
    64741 taccctcggt tgggcgctcc agcaggttta cacaacgtat aagcgtactc ttacccgcgc
    64801 cagaggcacc gataacgcca taaatttgtc cagctggcac atgcaggctg acgttgttca
    64861 acgcctggat ggtgcgggtg ccctggtgga acactttggt gatattcgaa agttttatca
    64921 ttgattattt attatcgtca ttaagttagt cgtggcattt cgaatgcctg gaacgggcaa
    64981 cgccttcaat gaaatggatg ttaaggcatc cagacgtcta aatcaatcag gtttatgcga
    65041 agagcacttt cttgctggtc gaaacatgcg atactagcgt cacatgcctt attaaggagc
    65101 tataaaaggt ggcgaagagc gtacccgcaa tttttcttga ccgtgatggc accattaatg
    65161 tcgatcacgg ctatgtccat gagatcgaca actttgaatt tatcgacggt gttattgacg
    65221 ccatgcgcga gctaaaaaaa atgggctttg cgctggtggt agtaaccaac cagtctggca
    65281 ttgctcgcgg taaatttact gaagcacagt ttgaaacgct gaccgagtgg atggactggt
    65341 cgctggcgga ccgcgatgtc gatctggatg gtatctatta ttgcccgcat catccgcagg
    65401 gtagtgttga agagtttcgc caggtctgcg actgccgcaa accacatccg gggatgcttt
    65461 tgtcagcacg cgattatttg catattgata tggccgcttc ttatatggtg ggcgataaat
    65521 tagaagatat gcaggcagca gctgcggcga acgtgggaac aaaagtgctg gtgcgtacgg
    65581 gtaaacctgt tacgcctgaa gcagaaaacg cggcggattg ggtgttaaat agcctggcag
    65641 acctgccgca agcgataaaa aagcagcaaa aaccggcgta atgattaaaa gatgagcggt
    65701 tgaaataaaa atgcattttt ccgcttgtct tcctgagccg actccctata atgcgcctcc
    65761 atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttaa agagaaaaat
    65821 cctgaaattc agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt
    65881 gagctgaaag ccgcgtcgc
//
LOCUS       DAZGAP010000003        64016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGAP010000003 DAZGAP010000000
VERSION     DAZGAP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.003"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(79..339)
                     /gene="yfhL"
                     /locus_tag="ACHLAM_000126"
     CDS             complement(79..339)
                     /gene="yfhL"
                     /locus_tag="ACHLAM_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO7851719.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(395..1243)
                     /locus_tag="ACHLAM_000127"
     CDS             complement(395..1243)
                     /locus_tag="ACHLAM_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO7851720.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1452..2087
                     /gene="yfhb"
                     /locus_tag="ACHLAM_000128"
     CDS             1452..2087
                     /gene="yfhb"
                     /locus_tag="ACHLAM_000128"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO7851721.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2145..2648
                     /gene="tadA"
                     /locus_tag="ACHLAM_000129"
     CDS             2145..2648
                     /gene="tadA"
                     /locus_tag="ACHLAM_000129"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO7851722.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2645..4201)
                     /gene="mltF"
                     /locus_tag="ACHLAM_000130"
     CDS             complement(2645..4201)
                     /gene="mltF"
                     /locus_tag="ACHLAM_000130"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO7851723.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4459..8346
                     /gene="purL"
                     /locus_tag="ACHLAM_000131"
                     /gene_synonym="purI"
     CDS             4459..8346
                     /gene="purL"
                     /locus_tag="ACHLAM_000131"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO7851724.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGASGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8903..10330
                     /gene="qseE"
                     /locus_tag="ACHLAM_000132"
     CDS             8903..10330
                     /gene="qseE"
                     /locus_tag="ACHLAM_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO7851725.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIDLPSSKNTK"
     gene            10495..11208
                     /gene="qseG"
                     /locus_tag="ACHLAM_000133"
     CDS             10495..11208
                     /gene="qseG"
                     /locus_tag="ACHLAM_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO7851726.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            11198..12532
                     /gene="glrR"
                     /locus_tag="ACHLAM_000134"
     CDS             11198..12532
                     /gene="glrR"
                     /locus_tag="ACHLAM_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO7851727.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12593..12931
                     /gene="glnB"
                     /locus_tag="ACHLAM_000135"
     CDS             12593..12931
                     /gene="glnB"
                     /locus_tag="ACHLAM_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO7851728.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(12976..14166)
                     /gene="hmpA"
                     /locus_tag="ACHLAM_000136"
     CDS             complement(12976..14166)
                     /gene="hmpA"
                     /locus_tag="ACHLAM_000136"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO7851729.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14495..15748
                     /gene="glyA"
                     /locus_tag="ACHLAM_000137"
     CDS             14495..15748
                     /gene="glyA"
                     /locus_tag="ACHLAM_000137"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO7851730.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15811..17004)
                     /locus_tag="ACHLAM_000138"
     CDS             complement(15811..17004)
                     /locus_tag="ACHLAM_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7851731.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17122..20403
                     /locus_tag="ACHLAM_000139"
     CDS             17122..20403
                     /locus_tag="ACHLAM_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO7851732.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKCSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            20500..21483
                     /locus_tag="ACHLAM_000140"
     CDS             20500..21483
                     /locus_tag="ACHLAM_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO7851733.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            21506..23017
                     /locus_tag="ACHLAM_000141"
     CDS             21506..23017
                     /locus_tag="ACHLAM_000141"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO7851734.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKDAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            23042..24040
                     /locus_tag="ACHLAM_000142"
     CDS             23042..24040
                     /locus_tag="ACHLAM_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7851735.1"
                     /translation="MSASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24073..25167
                     /locus_tag="ACHLAM_000143"
     CDS             24073..25167
                     /locus_tag="ACHLAM_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7851736.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25179..26051
                     /locus_tag="ACHLAM_000144"
     CDS             25179..26051
                     /locus_tag="ACHLAM_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO7851737.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDILC
                     LVYEHRSGVYHYRVSQTFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(26200..26622)
                     /locus_tag="ACHLAM_000145"
     CDS             complement(26200..26622)
                     /locus_tag="ACHLAM_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7851738.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26719..27921)
                     /gene="hcaD"
                     /locus_tag="ACHLAM_000146"
     CDS             complement(26719..27921)
                     /gene="hcaD"
                     /locus_tag="ACHLAM_000146"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO7851739.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            complement(27931..28743)
                     /gene="hcaB"
                     /locus_tag="ACHLAM_000147"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(27931..28743)
                     /gene="hcaB"
                     /locus_tag="ACHLAM_000147"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO7851740.1"
                     /translation="MSDLHNESIFITGGGSGLGLALAERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28740..29060)
                     /locus_tag="ACHLAM_000148"
     CDS             complement(28740..29060)
                     /locus_tag="ACHLAM_000148"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO7851741.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(29060..29578)
                     /gene="hcaF"
                     /locus_tag="ACHLAM_000149"
     CDS             complement(29060..29578)
                     /gene="hcaF"
                     /locus_tag="ACHLAM_000149"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO7851742.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(29575..30936)
                     /gene="hcaE"
                     /locus_tag="ACHLAM_000150"
     CDS             complement(29575..30936)
                     /gene="hcaE"
                     /locus_tag="ACHLAM_000150"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO7851743.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKHGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            31072..31962
                     /gene="hcaR"
                     /locus_tag="ACHLAM_000151"
     CDS             31072..31962
                     /gene="hcaR"
                     /locus_tag="ACHLAM_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO7851744.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            32122..33261
                     /locus_tag="ACHLAM_000152"
     CDS             32122..33261
                     /locus_tag="ACHLAM_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO7851745.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSSLTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33253..34533)
                     /gene="csiE"
                     /locus_tag="ACHLAM_000153"
     CDS             complement(33253..34533)
                     /gene="csiE"
                     /locus_tag="ACHLAM_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO7851746.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34724..35578)
                     /locus_tag="ACHLAM_000154"
     CDS             complement(34724..35578)
                     /locus_tag="ACHLAM_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7851747.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35723..36526)
                     /gene="suhB"
                     /locus_tag="ACHLAM_000155"
     CDS             complement(35723..36526)
                     /gene="suhB"
                     /locus_tag="ACHLAM_000155"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO7851748.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36645..37385
                     /gene="trmJ"
                     /locus_tag="ACHLAM_000156"
     CDS             36645..37385
                     /gene="trmJ"
                     /locus_tag="ACHLAM_000156"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO7851749.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37655..38143
                     /gene="iscR"
                     /locus_tag="ACHLAM_000157"
     CDS             37655..38143
                     /gene="iscR"
                     /locus_tag="ACHLAM_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO7851750.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38255..39469
                     /gene="iscS"
                     /locus_tag="ACHLAM_000158"
     CDS             38255..39469
                     /gene="iscS"
                     /locus_tag="ACHLAM_000158"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO7851751.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39497..39883
                     /gene="iscU"
                     /locus_tag="ACHLAM_000159"
     CDS             39497..39883
                     /gene="iscU"
                     /locus_tag="ACHLAM_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO7851752.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39900..40223
                     /gene="iscA"
                     /locus_tag="ACHLAM_000160"
     CDS             39900..40223
                     /gene="iscA"
                     /locus_tag="ACHLAM_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO7851753.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40319..40834
                     /gene="hscB"
                     /locus_tag="ACHLAM_000161"
     CDS             40319..40834
                     /gene="hscB"
                     /locus_tag="ACHLAM_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO7851754.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40851..42701
                     /gene="hscA"
                     /locus_tag="ACHLAM_000162"
     CDS             40851..42701
                     /gene="hscA"
                     /locus_tag="ACHLAM_000162"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO7851755.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42703..43038
                     /gene="fdx"
                     /locus_tag="ACHLAM_000163"
     CDS             42703..43038
                     /gene="fdx"
                     /locus_tag="ACHLAM_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO7851756.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            43050..43250
                     /gene="iscX"
                     /locus_tag="ACHLAM_000164"
     CDS             43050..43250
                     /gene="iscX"
                     /locus_tag="ACHLAM_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO7851757.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43309..44592
                     /gene="pepB"
                     /locus_tag="ACHLAM_000165"
     CDS             43309..44592
                     /gene="pepB"
                     /locus_tag="ACHLAM_000165"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO7851758.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44711..45487
                     /gene="sseB"
                     /locus_tag="ACHLAM_000166"
     CDS             44711..45487
                     /gene="sseB"
                     /locus_tag="ACHLAM_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO7851759.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(45519..45644)
                     /gene="timP"
                     /locus_tag="ACHLAM_000167"
     CDS             complement(45519..45644)
                     /gene="timP"
                     /locus_tag="ACHLAM_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO7851760.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
     gene            complement(46306..47151)
                     /gene="sseA"
                     /locus_tag="ACHLAM_000168"
     CDS             complement(46306..47151)
                     /gene="sseA"
                     /locus_tag="ACHLAM_000168"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO7851761.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            47358..52319
                     /gene="yfhM"
                     /locus_tag="ACHLAM_000169"
     CDS             47358..52319
                     /gene="yfhM"
                     /locus_tag="ACHLAM_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO7851762.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFTLWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            52320..54632
                     /gene="pbpC"
                     /locus_tag="ACHLAM_000170"
     CDS             52320..54632
                     /gene="pbpC"
                     /locus_tag="ACHLAM_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO7851763.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLALLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLAEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            54781..55212
                     /gene="ndk"
                     /locus_tag="ACHLAM_000171"
     CDS             54781..55212
                     /gene="ndk"
                     /locus_tag="ACHLAM_000171"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO7851764.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            55362..56516
                     /locus_tag="ACHLAM_000172"
     CDS             55362..56516
                     /locus_tag="ACHLAM_000172"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO7851765.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            56801..57808
                     /gene="rodZ"
                     /locus_tag="ACHLAM_000173"
     CDS             56801..57808
                     /gene="rodZ"
                     /locus_tag="ACHLAM_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO7851766.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            57835..58953
                     /gene="ispG"
                     /locus_tag="ACHLAM_000174"
                     /gene_synonym="gcpE"
     CDS             57835..58953
                     /gene="ispG"
                     /locus_tag="ACHLAM_000174"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO7851767.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARIGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            59064..60338
                     /gene="hisS"
                     /locus_tag="ACHLAM_000175"
     CDS             59064..60338
                     /gene="hisS"
                     /locus_tag="ACHLAM_000175"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO7851768.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRTLGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            60356..60976
                     /gene="yfgM"
                     /locus_tag="ACHLAM_000176"
     CDS             60356..60976
                     /gene="yfgM"
                     /locus_tag="ACHLAM_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO7851769.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            60987..62165
                     /gene="bamB"
                     /locus_tag="ACHLAM_000177"
     CDS             60987..62165
                     /gene="bamB"
                     /locus_tag="ACHLAM_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO7851770.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            62283..63755
                     /gene="der"
                     /locus_tag="ACHLAM_000178"
     CDS             62283..63755
                     /gene="der"
                     /locus_tag="ACHLAM_000178"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO7851771.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            63818..>64016
                     /locus_tag="ACHLAM_000179"
     CDS             63818..>64016
                     /locus_tag="ACHLAM_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO7851772.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRV"
BASE COUNT        15116 a        16076 c        17720 g        15104 t
ORIGIN      
        1 atgtcatcaa tcaaataaat ataatagcgt taaggtttct ctataaaata attaacactc
       61 tacatccagg gtagaatatt aaattttatc cgcgtgatgc ataagcacaa atttatccca
      121 caactgttct tccgtctcga catgttgcgg atccttcaca atagtattgg ggatcgggca
      181 caccttctgg caggttggtg tctcgtagtg ccctacgcat tcggtacact tatcgctgtt
      241 aatctcgtag atatgatcgc ccattgaaat cgcctcattc gggcattcgg gttcacacat
      301 atcacaattg atgcattttt tagtgattaa cagcgccata aggaaactct caaaacaaca
      361 caaaccgggc gggcattata cgcgcattct ttactcagac cagttttttc accagcgctt
      421 cactgtgacg aatacgttct ggagccagtt ccagatcctg ctgaatcagc gcaatgaaca
      481 gcaaatccgt taacattccc tgcgcgtgac aagcagagat tgaagcactg tttgtcgctt
      541 gttcttcggc aatggtatac aggcaatgag aagcacgctg ttgcagggca ttgggagtaa
      601 agccggtaat cgccagcact tttccgccca ctcgcagcat ctcatctgcc gccaggttta
      661 actcgcgtcg tacaccggtg taggaaatgg ctaataacag atcgtcaggg gacgacgcct
      721 gtactgtagc gagtagcgca tgcatatcgc gcactgcggc agcattgaag ccaatcttca
      781 tcagcttcca ggcaaagttt tgcgccacca gacccgaagc gccaataccg gtcagaatta
      841 tccgccgcgc agagcgcaac attgccacgc attcatgcag tttctcttca ctattaacgt
      901 ttaacgttgc gtacatagcg gcagtatttt ctttaatcag tttttcgccg accagccgta
      961 acggatcatc accgcggatt tggttatgaa tgggcacgga gggagattcc ggctggcttg
     1021 ccagcgcttc actcaacgcc aacttaagtg ccggaaaacc tttatagccg agtttttgcg
     1081 cgaacttcac gacgctggac tgactgactc cggcttcgtt ggccagttgc tgagagctta
     1141 aatggcgcgc cgtatcaggt tgtagcagca gataatccgc cagtttttta tcactttggg
     1201 caagcccctg gtaacgctga cggatacgaa gtaagccgtt catatctttc ctcgcgatga
     1261 aatagtggcg acaacaggca atatgagaca cacgcgagtg aattttatat tccattgccg
     1321 ctaattgcca tgaattaaaa attccagtta gccatgatgc ggtcgattgt tgccggaaaa
     1381 gtgatgatct atctgttagg ctatgtagta gcgaaaatta atcaggatgt ttcagtccag
     1441 aggagtatgg tttggcaact cacgagcgtc gtgtggtgtt ttttgactta gatggaacat
     1501 tgcatcagca ggatatgttc ggcagttttc tgcgttattt actgcgtcgc caaccgctga
     1561 atgcgttact tgtcctgccg ttgttgccga tcatagccat tgcgttattg ataaaaggtc
     1621 gtgcggcacg ctggccgatg agtctgcttc tgtggggatg cacttttggt cacaatgaag
     1681 cgcgtttgca ggcgttgcag gccgatttcg tacgctggtt tcgtgacaat gttaccgcct
     1741 ttccgctggt tcaggagcga ttaaccacct acctgttaag ttccgatgct gatatctggt
     1801 tgattaccgg ctctccgcag ccgctggttg aagcggttta tttcgatacg ccctggctgc
     1861 cgcgggttaa tcttatcgcc agccaaattc agcgtggcta tggtggttgg gtattgacga
     1921 tgcgttgtct gggacatgaa aaggtcgcac aactggagcg caaaatcggc acgccgctgc
     1981 ggctgtacag tggctatagc gacagtaatc aggacaatcc gctgctttat ttctgtcagc
     2041 atcgttggcg agtaaccccg cgcggtgaac tccagcaact ggaatagagt aaagcatagc
     2101 gtccgtgtat aatgcgccgc gcttttataa ccggagtttt ctttttgtct gaagtcgaat
     2161 ttagccacga atactggatg cgtcacgcgc tgacgctggc aaagcgtgcc tgggatgagc
     2221 gggaagtgcc ggtcggcgcg gtattagtgc ataacaatcg tgttattggc gaaggctgga
     2281 accgtccgat tggtcgacat gatcccaccg cacatgcaga aatcatggcc ctgcggcagg
     2341 gcggtctggt gatgcaaaat tatcgactga tcgacgccac gctgtatgtc acgcttgaac
     2401 cctgtgtaat gtgtgccgga gcaatgatcc acagtcgcat tggtcgcgtg gtctttggtg
     2461 cgcgtgacgc gaaaactggc gctgcgggat ctttaatgga tgtgctgcat catccgggta
     2521 tgaatcaccg ggtggaaatt accgaaggaa tactggcgga tgagtgcgcg gcgttgctca
     2581 gtgacttctt tcgcatgcgc cgccaggaaa ttaaagcgca gaaaaaagcg caatcctcga
     2641 cagattaatt ttgtttctct tcactccctt ttctggaaaa cagcagagag ggagagtggg
     2701 tcaaatagtt tgatgacgac tgggaaagaa acgagagaaa aggaaatttc tctttgccca
     2761 gctccgtagg cgataccgcc ggataatcct gcgccagttg catcgccgct tctgtagcct
     2821 gcttctcttt ctcctgcaga taacccacca ggctaatctg atacttacga atattttcga
     2881 cataagcgta ggcttcatgc ccacgagcgt agccgtaagt cagcttgctg taatagggtt
     2941 tctggctaag taaaggcaga cgctgtttta cgtcagccca actgtcagga ttacctttgg
     3001 tttttgccgt cagggcgcgg gcatccagca tatgcgcata gcccatattg tacgcggcaa
     3061 gggcaaacca gatccgttcg ttctccggca cactttccgg cactttactc atcatatcct
     3121 gcaaataacg cacgccgccg ctgatgctct gttcggcatc ggtacgatcc gtaatgccga
     3181 ggctttgcgc ggtgtttttg gttaacatca tcatgccgcg cacacccgtc ggtgaagtgg
     3241 cctgagcatc ccagtgcgat tcttgataag caatagcggc cagcaaacgc cagtcaattt
     3301 cttcggcgta tttctcaaac aggggcttta actgcggcag taccgcatcg acggcgcgta
     3361 aaaatgtgcg cgtatcgacg taatcaaaat catcgccatg ccccaggtat ttctcttcaa
     3421 tgcgtgccag cgtaccgtct tcattcatct cattgaagaa atcgagcaga gcggcggaaa
     3481 gggtattatc gccatctaac gggctaaacc aggtcaccgg ttgttcatcg gtgatatcga
     3541 gcgctacggc gagttccggg tgaacgcgct gaaacaggct gatggcgaca gaatcagcaa
     3601 tggtgtaatc gagttttcct tcgatgacat cttccattaa ttccgcagag ccttttttgt
     3661 cgtctacctt ccagcttaat tccgggaatt ttgtttcttt cagggtctgg agatcgttaa
     3721 ccaccacatg acccggtgca acggtgagtt gctccgccgt caggttgccc agtgtacgtg
     3781 ggcgatactg acccacttta taaaccagtt gttgtgacac ggaataatag gtagggccag
     3841 gctgataatt ttttacacgc tcactgttat agaccaggcc tgccgccagc aggtcggcgt
     3901 taccattatc gaggtcgtca aacagctggc tgatattctg ccgcacggtc actttcagtt
     3961 ttacgccgag gtaatcggca aactgtttcg ccagttcgta atccaggcca aaaggtttcc
     4021 cgttgatttc gttataagtc aggggggtat gaatggtgct cacacgcaac tctccccgcg
     4081 cttgaatggc ggcgatacgg ttgtcggctt taccaaacca gggaatggat ggccagagag
     4141 cgaccgcgag cagcagtgcc agaatgccga tgaacagata attaatcttt aattttttca
     4201 attagttaat tctctgtgtc gtgcgcgtcc cagcttgaaa aaacgtaata atagtgaaag
     4261 gtttactcat aaatgagcgg cattttgcgt aaacctgcgc cagttggcaa cttattacag
     4321 ccattggcgg cacgcgttgc taattcacga tggtgatttt atttccacgc aaacggtttc
     4381 gtcagcgcat cagattcttt ataatgacgc ccgtttcccc cccttgggta caccgaaagc
     4441 ttagaagacg agagacttat gatggaaatt ctgcgtggtt cgcctgcatt gtcggcattc
     4501 cgaatcaaca aactgctggc acgttttcag gctgccaggc tcccggttca cactatttac
     4561 gccgagtatg tccattttgc tgacctcaat gcgccgttaa acgatgatga gcacgcacaa
     4621 cttgaacgcc tgctgaaata tggcccggca ctcgccagcc acgccccgca aggcaaactc
     4681 ctgctggtga ccccgcgtcc tggcaccatc tctccctggt cttcgaaagc gaccgatatc
     4741 gcccataatt gcggtctaca acaggtaaac cgccttgagc gcggcgttgc ttactatgta
     4801 gaagccggta cgctgaccaa tgaacaatgg cagcaggtta ccgctgaact gcacgaccgc
     4861 atgatggaaa cggtcttttt tgctttagat gatgcagagc agctgtttgc acaccatcaa
     4921 ccgactccgg tcaccagcgt tgatttgctg gggcagggcc gtcaggcgct aatcgacgct
     4981 aacctgcgtc ttggcctggc actggcggaa gatgaaattg actatttgca ggatgctttc
     5041 acaaagctgg gtcgtaaccc gaacgacatc gaactgtata tgtttgccca ggcgaactcc
     5101 gagcactgcc gccacaaaat ttttaacgcc gactgggtta tcgatggtga acagcagccg
     5161 aaatcactgt ttaagatgat caaaaacacc ttcgaaacca cgccggatca cgttctctct
     5221 gcttataaag ataacgccgc cgtaatggaa ggttctgaag tgggccgcta ctttgctgac
     5281 cacgaaacgg gccgctacga tttccatcag gaacctgcgc atatcctgat gaaagttgaa
     5341 actcacaacc acccgacggc gatttctccg tggccgggcg cggcgaccgg ttccggcggt
     5401 gaaatccgcg atgaaggtgc ctccgggcgc ggcgcgaagc cgaaagctgg tctggtcggt
     5461 ttctccgtgt ccaacctgcg aattcctggc ttcgaacagc cgtgggaaga agatttcggt
     5521 aaacctgagc gcattgttac cgcgctggac atcatgaccg aaggcccgct gggcggtgcg
     5581 gcgtttaaca acgaatttgg tcgtccggca ctgaatggct acttccgtac ttatgaagaa
     5641 aaagtgaaca gccacaatgg cgaagagctg cgcggttatc acaaaccgat catgctggcg
     5701 ggcgggatcg gcaacattcg tgccgatcac gtacaaaaag gcgagatcaa cgttggtgcg
     5761 aagctggtcg ttctcggcgg cccggcaatg aacatcggtc ttggcggcgg cgcggcgtct
     5821 tccatggcgt ctggtcagtc tgatgccgac ctcgacttcg cttccgtaca gcgcgacaac
     5881 ccggagatgg agcgtcgctg ccaggaagtg atcgaccgtt gctggcagct tggtgatgcc
     5941 aacccaatcc tgtttatcca cgacgttggc gcaggcggtc tttctaacgc catgccggaa
     6001 ctggtgagcg acggcgggcg cggcggtaaa ttcgaactgc gcgatatcct gagcgacgaa
     6061 ccgggcatga gcccgctgga aatctggtgt aacgaatccc aggaacgcta cgtgctggca
     6121 gttgctgccg atcagttacc gctgtttgac gaactgtgta agcgtgagcg cgcgccatac
     6181 gcggtgattg gtgaagcgac cgaagaactg catctttctt tgcacgatcg tcattttgat
     6241 aatcagccga tcgatctgcc gctggacgta ctgcttggta aaacgccgaa gatgacccgc
     6301 gaagtacaaa cgctgaaagc gaagggtgat gcgctggttc gtgaagggat taccattgct
     6361 gacgcggtga aacgcgtgct gcacctgccg accgtggcgg agaaaacttt cctggtgacc
     6421 attggtgacc gcagcgtaac cggcatggtg gcgcgcgatc agatggtggg tccgtggcag
     6481 gtgccggtcg ctaactgcgc ggtcactacc gccagcctcg atagctacta cggtgaagcg
     6541 atggcgattg gcgagcgtgc gccggttgcg ctgctggatt tcgccgcttc tgcccgtctg
     6601 gcggtcggtg aagcgttaac caacatcgcc gcaacacaaa ttggcgatat caaacgcatt
     6661 aaactttccg ccaactggat ggcggcggca ggccaccctg gtgaagatgc aggcctgtat
     6721 gaagccgtta aagccgtggg tgaagaactt tgtccggcgc tgggcctgac gatcccggtg
     6781 ggtaaagact ctatgtcgat gaaaacccgt tggcaggaag gtaacgaaga gcgcgaaatg
     6841 acgtcgccgc tgtcgctggt gatttctgca tttgcccgcg tggaagatgt acgtcacacc
     6901 atcacgccac agctttctac cgaagataac gcactgctgc tgattgattt gggcaaaggc
     6961 aataacgcgc tgggcgcaac ggcgctggcg caggtttatc gtcagcttgg cgacaaaccg
     7021 gcagatgtac gcgatgtcgc gcaactgaaa ggcttctatg acgcgattca ggcgctggtt
     7081 gcacagcgta agctgctggc gtatcacgac cgctctgatg gcggcctgct ggtaacgctg
     7141 gcggaaatgg cctttgctgg tcattgcggc attgacgcgg atatcgcctc tctgggtgac
     7201 gatcgcctgg cggcgttgtt taacgaagaa ctgggtgcgg tgattcaggt tcgtgccgct
     7261 gaccgtgaag ccgttgaagc ggtactggca cagcatgggc ttgcggattg cgtccattat
     7321 gtagggcagg cggtttccgg tgaccgtttt gtgattaccg ccaacgggca gactgtattc
     7381 agcgaaagcc gcactacgtt gcgtgtctgg tgggcagaaa ctacctggca gatgcagcgc
     7441 ctgcgtgaca acccggagtg tgccgatcag gagcaccagg cgaaatcaaa cgacgccgat
     7501 ccgggcctga atgtgaaact gtcgttcgat atcaacgaag atgtggcagc accgtatatt
     7561 gccactggtg cgcgtccgaa agttgccgtg ctgcgtgagc agggggtgaa ctcgcatgtt
     7621 gaaatggcgg cagctttcca ccgtgcaggc ttcgatgcaa tcgatgtgca tatgagcgac
     7681 ctgctggctg gacgcacggg cctggaggat ttccacgctc tggtagcgtg cggtggattc
     7741 tcctacggtg acgtgctggg tgctggtgaa ggctgggcga agtctatcct gttcaatgac
     7801 cgtgtacgcg atgagtttgc aaccttcttc caccgtccgc aaacgctggc gctgggggta
     7861 tgtaacggtt gccagatgat gtctaaccta cgcgaactga tcccaggtag tgagttgtgg
     7921 ccacgttttg tgcgcaatac ctccgatcgc tttgaagcgc gtttcagcct ggttgaagta
     7981 acccaaagcc cgtctctgct gttgcagggg atggtgggct cgcaaatgcc gattgctgtc
     8041 tctcatggcg aagggcgcgt ggaagtgcgt gatgcggcgc atctggcggc actggaaagc
     8101 aaagggctgg tggcactgcg ctatgtcgat aacttcggca aagtcactga aacctacccg
     8161 gctaacccga acggttctcc gaatggtatt acggcagtca cgactgaaag tggtcgagtc
     8221 accattatga tgccgcaccc ggaacgtgtt ttccgtactg tcagcaactc ctggcatccg
     8281 gaaaactggg gcgaggatgg gccgtggatg cgtattttcc gcaatgcacg taagcagttg
     8341 gggtaagccg tcaacccatt ggttttgcag ccggatgagg cattaagcac caactactat
     8401 tataagctcc caacttcggg agcttttttg tgtctgtaaa tcacgacaat gggtggttta
     8461 ccgcgtcgct ttctggcgac acttaactaa ttgattttaa tggcatctaa taagtttatc
     8521 ttaagttgtc tctttttagc gacacagtgg ctgatttaca gccaaatagc gaagaagcca
     8581 taaccccttc aaaacagcgt acaatcagta cgttaccaaa ctattttctt tattggcaca
     8641 gttactgcat aatagtaacc agtggctcat tcaccgactt atgtcagccc cttcgggacg
     8701 tgctacataa aatacgaatg acgcacaaca aggtgcctgc cgtccaactt ctgatatcag
     8761 cgtagctata tcaaccatcg ggcgaaacgt cgagttaggc accgccttat tccataacaa
     8821 agccgggtaa ttcccggctt tgttgtatct gaacttcccc tcggttagca tcaggctatt
     8881 cgcgtctgac gagagtaaca ccttgaaacg ctggcccgtt tttccccgct cattacgaca
     8941 actggtaatg ctggcatttt tgctgattct gctgcccctg ttggtgctgg catggcaagc
     9001 ctggcaaagc ctgaatgcgc ttagcgatca ggcggcgctg gttaaccgca ctacgcttat
     9061 tgatgcccgg cgtagtgaag cgatgaccaa cgcggcgctg gagatggagc gtagctaccg
     9121 tcagtattgc gtgctggacg accctacgct tgcgaaggtt tatcaaagcc agcgcaagcg
     9181 ttacagcgaa atgctcgatg cccacgcagg cgtactgcca gacgataaac tttaccaggc
     9241 attgcgtcag gatttgaaca atctggccca acttcagtgt aacaacagtg gtcccgatgc
     9301 tgccgccgcc gcgcgtcttg aagcctttgc cagtgccaat accgaaatgg tgcaggccac
     9361 gcgcacagtg gtgttctctc gtgggcagca acttcagcgt gaaatcgccg aacgtgggca
     9421 atattttggt tggcaatcgc tggtgctatt tctggtgagt ctggtgatgg tgctactttt
     9481 cacgcggatg attatcgggc cggtgaaaaa tatcgagcgc atgatcaacc gtctggggga
     9541 agggcgttct ctgggcaata gcgtttcgtt cagtggaccg agcgagttac gctcggttgg
     9601 gcaacgtatt ctttggttaa gtgagcgcct gtcatggctg gaatcccaac gccatcaatt
     9661 tttaagacat ttatctcatg aattaaaaac gccactggcg agtatgcgcg agggcactga
     9721 attactggct gaccaggttg tcgggccgct tacgccagag caaaaagagg tggtgagcat
     9781 tcttgatagc agcagccgca atttgcaaaa actgatcgaa caactgcttg attacaaccg
     9841 taaacaggcg gacagtgcgg tggaactgga gaatgttgaa ttagcaccgc tggtggagac
     9901 agtggtttct gctcatagcc tgcccgcacg ggctaaaatg atgcataccg acgtcgatct
     9961 caaagcaaca gcttgcctgg cggagccaat gctgctgatg agcgtactgg ataatcttta
    10021 ctccaatgcg gtgcactacg gggctgaatc cggtaacatt tgccttcgca gcagttcaca
    10081 tggtgcgcgg gtttatattg atgtcatcaa tacaggcacg cccattccgc aagaggaacg
    10141 cgccatgatc ttcgaaccct tttttcaggg aagccaccag cgaaaagggg cggtgaaggg
    10201 cagcggtctg ggattaagca ttgccaggga ttgtattcgc cgtatgcaag gggaactgta
    10261 tctggtcgac gagagcgggc aagacgtttg tttccgcatt gatttaccgt cgtcgaaaaa
    10321 cacgaaataa tgctaacagc ccatcccatc aggctatttt atttgtcagt tcggccccgg
    10381 gcagtgctcg aaatcttcac gtacgacatg tacgctctgg tttctccgcg ctgtccatgt
    10441 ccaaactgcc tgcaacaatt acgcccactg ggataggctt taagtctggt gaatatgcga
    10501 cacatttttc aacgattatt gccccgacgg ctgtggctgg cagggctgcc atgtctggca
    10561 ttgctgggtt gcgtgcagag tcacaataag ccagccattg atacgccagc agaagaaaaa
    10621 attccggttt atcaactggc tgactatctt tctactgaat gtagcgacat ctgggcgctg
    10681 caaggtaaat caaccgaaac caatccgctt tactggctgc gggcgatgga ttgtgctgat
    10741 cgtttaatgc ctgcgcagtc gcgtcagcaa gcccgccaat atgacgacgg caactggcaa
    10801 aataccttta aacaagggat tttgctcgcc gacgccaaaa ttacgccgta tgagcgccgc
    10861 cagctggtcg cgcgcattga tgcgctaagt accgagatcc cggcacaggt tcgccctctg
    10921 tatcaactct ggcgcgacgg tcaggcgctg caactgcaac tggcggaaga gcgccaacgc
    10981 tacagtaaac tccagcagtc cagcgacagc gagctggata ctttgcgcca gcaacatcac
    11041 gttctacaac agcaactgga actcactacc cgcaagctgg aaaacctgac cgatattgaa
    11101 cgccagctct cgacccgcaa accggctgga aatttctcgc cggatacgcc gcacgagagt
    11161 gaaaagcccg cgccatccac tgatgaggtc actcctgatg agccataaac ctgcgcattt
    11221 attactggtc gatgacgatc cgggattgct gaaactgctt ggcctgcgcc tgaccagcga
    11281 aggctacagc gtggtcacgg cggaaagtgg tgctgaagga ttacgggtac tgaatcgcga
    11341 aaaagtagat ttagttatca gcgacctgcg gatggatgaa atggatggta tgcagctgtt
    11401 tgctgaaatc cagaaagtgc agccgggaat gccggtaatt attcttaccg cacatggttc
    11461 tattcccgat gccgttgctg caacacagca gggagtgttc agtttcctca ccaagcctgt
    11521 cgataaagac gcgctatatc aggcaattga cgatgcgctg gaacaatccg cgccagccac
    11581 cgatgaacgc tggcgcgagg cgattgtcac ccgcagcccg ctgatgctgc gtttgctgga
    11641 acaggcgcgg ctggtggcgc aatcagacgt tagcgttttg attaacggtc agagcggcac
    11701 cgggaaagag attttcgccc aggctatcca caacgccagc ccgcgcaata gcaaaccatt
    11761 tattgctatt aactgtggtg cattgcccga gcaattgctg gagtcggagc tgtttggtca
    11821 tgcgcgtggc gcgtttactg gcgctgtcag caatcgcgaa ggtttattcc aggcggcgga
    11881 aggcggtacg ctatttctcg atgagattgg cgatatgcct gcaccgttgc aggtcaaact
    11941 gctgcgtgtg ttgcaggagc gtaaagtccg cccgctgggc agtaaccgcg atattgatat
    12001 cgacgtgcgg attatttccg ccactcaccg cgacctgcca aaagcgatgg cgcgcgggga
    12061 attccgtgaa gatctctatt accgcctcaa cgttgtcagc ctgaaaattc cggctctggc
    12121 agagcgcaca gaagacattc cgctactggc aaatcacctg ttgcgccagg cggcagagcg
    12181 acataaaccg tttgtccgcg cgttctccac cgatgcgatg aaacgcctga tgaccgctag
    12241 ctggccgggt aatgtacgcc agttggtcaa cgtgattgaa cagtgcgtgg cgctgacctc
    12301 atctccggtg attagcgatg cattagtaga gcaggcgctg gagggcgaaa acacggcgct
    12361 gccaaccttt gttgaggcac gtaatcagtt tgaactcaac tatttgcgta agctgctgca
    12421 aatcaccaaa ggcaacgtca cccacgcggc gagaatggcg gggcgcaacc ggacagaatt
    12481 ttataaactg ctttcccgac acgagctgga tgcaaacgat ttcaaggaat gaattggcgt
    12541 tatgtgttac gtttagcaga tcaaaagaca ggcgaccttt tcaaggaata gcatgaaaaa
    12601 gattgatgcg attattaaac ccttcaagct ggacgatgtt cgcgaagcac tggctgaagt
    12661 cggcattacc ggcatgacgg tcactgaggt gaaaggtttt ggccgccaga aaggccatac
    12721 cgagctgtac cgcggcgcgg agtatatggt ggattttctg ccgaaagtga aaattgagat
    12781 tgtcgtaccg gacgacattg tcgatacctg tgtcgatacc attattcgca cggcgcaaac
    12841 cggcaaaatc ggtgacggta aaatcttcgt ctttgacgtg gcacgggtca ttcgtatccg
    12901 taccggtgaa gaagacgacg cggcaattta aaccgcgtca agggttgccg gatgtttcca
    12961 tccggcaaca tcaaatcaca gcaccttatg cgggccaaag cattcgtaat gaatattttc
    13021 ctgcttcacg cccagatcca ctaactgttt cgcggcaaac tgcatgaagc caaccgggcc
    13081 gcagagatag aactgcattg tcggatcgct gaacgcacct tccagtttgc tcaaatccat
    13141 cagaccttcg ctatcaaact gacctttagc gcgatcggct tcgctcggct gacgatacca
    13201 ggtgtgcgcg gtaaagcgcg gcagtgacag ccccagttcc ttaacttcat cagcaaaggc
    13261 gtgaacatcg ccattttctg ccgcatggaa ccagttcact tgtgctgtgt ggcctgcttt
    13321 tgccagtgtg tcgagcattg ccagcattgg cgtttgaccg acaccggcag agattaacgt
    13381 cactggtgtg tcatctgcga cagccataaa aaaatcacct gctggagcga ccagtttcac
    13441 gacatcgcca acattggcgt gattgtgcag ccagttggat acctgcccac cctcttcgcg
    13501 tttcaccgca atacgatagc ctttgccatc cggtttgcga gtcaaagagt actgacgaat
    13561 ttcctgatgc gggaaacctt ccggcttcag ccagacgccg agatattgcc ccggacggta
    13621 ttctgccact gcgccaccgt cgaccggctc cagttcgaag ctggtgataa gcgcgctgcg
    13681 cggtgtttta gccacaatgc ggaaatcgcg agtaccttcc cagccaccgg ctttgctggc
    13741 gttttcgtta tagatttccg cctcgcgatt gataaataca ttagccagta cgccataggc
    13801 tttaccccac gcgtccagca cttcctggcc cgggctgaac atttcgtcca gcgtcgccaa
    13861 caggtgttca ccgacaatgt tgtactgttc cggtttgatc tggaagctgg tgtgcttctg
    13921 cgcgattttt tctaccgctg gcagcagcgc aggcaggttt tcaatattac tggcgtaggc
    13981 ggcaatagcg ttaaacaggg cttcacgttg atcgccattg cgctggttac tcatgttaaa
    14041 aatttctttg agttctgggt tatgagtaaa catacggtcg tagaaatggg cggttaactt
    14101 tggtcccgtt tccaccagta aagggatggt ggcttttact gtagcgatgg tttgagcgtc
    14161 aagcatatgg tcttcctttt ttttgcatct taattgatgt atctcaaatg catcttataa
    14221 aaaatagccc tgcaatgtaa atggttcttt ggtgtttttc agaaagaatg tgatgaagtg
    14281 aaaaatttgc atcacaaacc tgaaaagaaa tccgtttccg gttgcaagct ctttattctc
    14341 caaagccttg cgtagcctga aggtaatcgt ttgcgtaaat tcctttgtca agacctgtta
    14401 tcacacaatg attcggttat actgttcgcc gttgtccaac aggaccgcct ataaaggcca
    14461 aaaattttat tgttagctga gtcaggagat gcggatgtta aagcgtgaaa tgaacattgc
    14521 cgattatgat gccgaactgt ggcaggctat ggagcaggaa aaagtacgtc aggaagagca
    14581 catcgaactg atcgcctccg aaaactacac cagcccgcgc gtaatgcagg cgcagggttc
    14641 tcagctgacc aacaaatatg ctgaaggtta tccgggcaaa cgctactacg gcggttgcga
    14701 gtatgttgat atcgttgaac aactggcgat cgatcgtgcg aaagaactgt tcggtgctga
    14761 ctacgctaac gtccagccgc actccggctc ccaggctaac tttgcggtgt acaccgcgct
    14821 gctggaacca ggtgataccg ttctgggtat gaacctggcg catggcggtc acctgactca
    14881 cggttctccg gttaacttct ccggtaaact gtacaacatc gttccttacg gtatcgatgc
    14941 tagcggtcat atcgactacg ccgatctgga aaaacaagcc aaagaacaca aaccgaaaat
    15001 gattatcggt ggcttctctg catattccgg cgtggtggac tgggcgaaaa tgcgtgaaat
    15061 cgctgacagc atcggtgctt acctgttcgt tgatatggcg cacgttgcgg gcctggttgc
    15121 agctggcgtc tacccgaacc cggttcctca tgctcacgtt gttactacca ccactcacaa
    15181 aaccctggcg ggtccgcgcg gcggcctgat cctggcgaaa ggtggtagcg aagagctgta
    15241 caaaaaactg aactctgcgg ttttccctgg tggtcagggt ggtccgttga tgcacgtaat
    15301 cgccggtaaa gcggttgctc tgaaagaagc gatggagcct gagttcaaaa cttaccagca
    15361 gcaggtcgct aaaaacgcta aagcgatggt agaagtgttc ctggaacgcg gctacaaagt
    15421 ggtttccggc ggcactgata accatctgtt cctggttgat ctggttgata aaaacctgac
    15481 cggtaaagaa gctgacgccg ctctgggccg tgctaacatc accgtcaaca aaaacagcgt
    15541 accgaacgat ccgaagagcc cgtttgtgac ctccggtatt cgcgtgggta ctccggcgat
    15601 tacgcgtcgc ggcttcaaag aagcagaagc gaaagaactg gctggctgga tgtgtgacgt
    15661 gctggacagc atcaatgatg aagccgttat cgagcgcatc aaaggtaaag ttctcgacat
    15721 ctgtgcacgt tacccggttt acgcataagc gaaacggtta atctgttgaa aaaggcttcc
    15781 ttcgggaagc ctttctacgt tatcgcgcca tcaaatctgt cgtaactgcg cctcaacata
    15841 caaatagcca atccccagca cctgttgtgc gcggcttaat tgcccaaagc caattttcgt
    15901 cgctttcacg ctcggtcctt cgtcgcggtc gaacgggtta aatcccgtct ggcgaataat
    15961 ggtattaagc cagtgctcac caaaggcaca actgcgaccg tacaaccaaa tctgattaat
    16021 attgaggatg ttgaggaagt tatacagact caaaccaatg gcattggcag agcgatcaac
    16081 ccagctggtg atccacggtt ctccactctg ccaggcagcg attaactgcg ctgttgtcag
    16141 tttttcagga tcaagctgag tattaaccgg ttgtgatttt agccataccc gcgcctgttt
    16201 ttttaatgca cttaacgagg cgacggtttc caggcagccg taacgtccac agtcgcagac
    16261 gacgccatcc ggattaacga tggtgtgacc aatctgtccg ctaccataca aacagccgcg
    16321 ataaatttgc tcgttaatca cgaacgacga gccaatgccg taatcaacgt tgatcacgca
    16381 gaaatcccgt tcctgcgaat tattttgcca tttctccgcc agcgccagca tcacgcagtc
    16441 attatcgacc atcacccgaa tgccgagctt ctcttccagc agatacttta cctcaaccgg
    16501 cgttgtccac ggcgcttgcg gcatggtttg cgacacgccg gtcaccggat caacctgacc
    16561 gtggattgcc agcgccaggt tgatggtacg gtccggccac aatttacggt ggcgatgcca
    16621 gcatttttcg atttcggaca gcagcgcctg cggcgtcggt gcatcaatct gtaaatactc
    16681 aaattcacct ttcggactta aacaagcatt agcaacctga cactcaatac tggtgggcgt
    16741 cacgttcagg cacaacgtcc agtcaccttc cggcgcaatc agccatgtac cgctactatg
    16801 cccgcgcgtc tggctttcat cgtcgatatt caccacccgt ttttcgcttt ccagttcctg
    16861 caaaatatta ctgaccgccg gaatcgaaat ttgcgccagc cgggccagcg ttgatttatt
    16921 ggcgcgcttt tgccggtaca gcagttccag aatcacgcat ttgttatggt ggcgaatctg
    16981 ttgattatta atgcaggctc tcatcttcac taaaccttgt ttagttaatt gcgtgattat
    17041 tccgcgaaaa cattgccaga ttgttcaata cactgccaca aatcttttaa ttcagtatgt
    17101 cttgttaata ttgagggcac catgactcca gtaaaagtgt ggcaagagcg cgttgagatc
    17161 ccgacctatg aaaccgggcc gcaggatata catcccatgt tcctggaaaa tcgcgtttat
    17221 cagggatcgt ccggcgcggt ttatccctac ggcgtgaccg atacgctgag cgagcagaaa
    17281 accctgaaat cctggcaggc ggtgtggctg gaaaacgact acatcaaagt gatgatcctg
    17341 ccggagctgg gcggtcgggt gcatcgcgcg tgggataaag tgaaacagcg cgattttgtc
    17401 tatcataatg aagtcattaa accggcgctg gtggggttgc tggggccgtg gatctccggc
    17461 gggattgagt ttaactggcc gcaacaccat cgcccgacca cctttatgcc cgtcgatttc
    17521 accctcgaag cccatgaaga cggcgcacaa accgtgtggg tcggcgaaac ggagccgatg
    17581 cacggtttac aggtgatgac aggtttcacc ctgcgccctg accgggcggc actggaaatt
    17641 gccagccgcg tctataacgg caacgccacg ccgcgtcatt tcttgtggtg ggccaacccg
    17701 gcagtgaaag ggggtgaagg gcatcagagc gtcttcccgc cggatgtaac ggcggtgttt
    17761 gatcacggca aacgggccgt ctccgctttc cccatcgcca ccggcactta ctacaaagtg
    17821 gactactccg ccggagtgga catttctcgc tataaaaatg tgcccgttcc aacctcatat
    17881 atggctgaaa aatcacagta cgattttgtt ggcgcgtggt gtcacgatga agatggcggt
    17941 ttgctgcacg ttgccaacca ccatattgcg ccaggtaaaa aacagtggag ctggggacac
    18001 agtgaatttg gccaggcgtg ggataagagt ctgaccgaca ataacggccc gtatatcgaa
    18061 ctgatgactg gtatttttgc cgataaccag cccgatttta cctggcttga tgcttacgaa
    18121 gagaagcgtt ttgagcagta tttcctgcct taccattctt tgggcatggt gcaaaacgcc
    18181 tcccgcgatg cggtgattaa actccagcgt agcgagcggg ggattgagtg ggggctgtat
    18241 gccatctctc cgttgaaagg ataccgtctg gcgatccgcg aaatcggcaa atgcaacgcg
    18301 ttgctcgatg atgccgtggc cctgacacca gcgaccgcca tccagggcgt gttgcacggt
    18361 atcaatcctg aaaggctgac cattgaactc tccgatgccg acggcaatat cacactgagt
    18421 tatcaggaac atcagccgca agagttgccg ttgccggacg tcgccaaagc gccactggca
    18481 gcacaagaca ttaccagtac agatgaagcc tggtttatcg gtcagcatct ggagcaatat
    18541 catcacgcca gccgttcacc gttcgattac tacctgcgcg gcgtggcgct ggatccgctg
    18601 gattatcgct gtaacctggc gctggcgatg ctggaatata accgagcaga tttcccgcaa
    18661 gcggtggcgt atgccactca ggcgctgaaa cgcgcacatg cgctaaacaa aaatccgcag
    18721 tgcggacagg cgagtttgat tcgcgccagt gcttacgaac gtcaggggca atatcaacaa
    18781 gccgaagagg atttctggcg ggcggtctgg agcggcaaca gtaaagcagg tggctattat
    18841 ggtctggcac ggctggcggc gcgtaatggt cacttcgacg ctggtctgga tttttgccaa
    18901 caaagtcttc gcgcctgccc aaccaatcag gaagtgcttt gcctgcataa tctgctgctg
    18961 gtgttaagtg gtcgtcagga caacgcgcgt ttgcagcgcg agaaactgct gcgtgattat
    19021 ccgctgaacg ccactctgtg gtggctgaac tggttcgatg gtcgtagcga atcagccctc
    19081 gcgcagtggc gcggtctgtg tcagggacgc gacgttaacg ccctgatgac cgccgggcaa
    19141 ctgattaact ggggaatgcc caccctggcg gcagagatgc tgaacgcact ggactgccag
    19201 cgcacgctgc cgctttactt gcaagccagc ttgctgccga aagctgaacg cggcgaactg
    19261 gtcacaaaag ccattgatgt cttcccacag tttgtccgtt tcccgaatac gctggaagaa
    19321 gtggcggcgc tggagagtat tgaagagtgc tggtttgccc gtcatttact ggcctgcttc
    19381 tactacaaca agtgcagcta caacaaagcc attactttat ggcaacgttg cgtagagatg
    19441 tcgccggagt ttgccgacgg ctggcgcggg ttagcgatcc atgcgtggaa taagcaacac
    19501 gattatgagc tggccgcgcg ttatcttgat aatgcttatc agcttgcgcc gcaggatgca
    19561 cgtctgcttt tcgaacggga tttgctggat aagttaagcg gtgtcacacc ggagaaacga
    19621 ctggcgcgtc tggaaaataa tctggaaatt gcgctgaaac gcgacgacat gaccgcagaa
    19681 ctgctcaatt tgtggcatct cacgggtcag gcagacaaag cggcggacat tctcgccacg
    19741 cgcaaattcc acccgtggga aggcggggaa gggaagatca ccagtcagtt tatcctcaac
    19801 cagttattac gcgcctggca gcatcttgat gccagagagc cgcagcaggc cagcgaactg
    19861 cttcatgccg cgttgcatta tccggagaat ttaagcgaag gccgtttacc ggggcaaact
    19921 gacaacgaca tctggttctg gcaggcgata tgcgccaacg cgcagggcga tgaaactgaa
    19981 gcgacgcgtt gtttacgtct ggcggcgact ggcgatcgca ccattaacat tcacagttat
    20041 tacaacgatc agccggttga ttatctcttc tggcaaggaa tggcgctgcg actgctgggc
    20101 gaacaacaca ccgcacagca actgtttagt gaaatgaaac agtgggcgca agagatggcg
    20161 aaaaccagta tcgaggcgga tttctttgct gtttcacaac ctgacctgtt gtcgctgtat
    20221 ggcgatttac aacagcagca taaagaaaaa tgcctgatgg tggcgatgct ggcgtccgcg
    20281 ggactcgggg aggttgcgca ctatgaatct gctcgcgctg aattgatggc gattaatcca
    20341 gcctggccga aagcggcatt attcaccacc gtgatgcctt ttatttttag ctacgttcac
    20401 taaaccttat taactgtaat atttgagcgg cacgcgctgc cgctcacgct caaataatta
    20461 aaataagccg ttgtaaataa aaaccctaca ggaggcacta tgcctaaaaa aatgacaaga
    20521 acccgtaatt tattattaat ggctacgcta ttgggtagcg cactatttgc cagggctgcg
    20581 gataaagaaa tgactattgg cgcaatatac cttgataccc agggatatta cgccggagtg
    20641 cgtcagggcg ttcaggatgc ggcaaaagat tcttcagtac aggtacagtt aattgaaact
    20701 aacgctcagg gcgatatttc gaaagaaagt acctttgttg ataccctcgt ggcgcgtaat
    20761 gtcgatgcca ttattttatc ggcagtgtct gaaaatggca gtagtcgtac cgttcgtcgc
    20821 gccagtgaag cgggtattcc ggtgatttgc tacaacacct gtattaatca aaaaggtgtc
    20881 gataaatatg tctcggcgta tctggtcggc gatccgctgg aatttggtaa aaaactgggt
    20941 aacgctgccg ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc
    21001 aattgcgaag cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga ggtattaaaa
    21061 tcccgcgttc ccggcgcgca aatagtcgct aatcaggaag ggactgtttt agataaagcg
    21121 atttccgttg gtgaaaaact aattatctcc acgccggatc tcaacgccat tatgggggaa
    21181 tcgggcggtg cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa
    21241 attgctgttt tcggttcgga tatgaccact gaaattgctc aggagctgga aaacaatcag
    21301 gtgctgaaag cggtggtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa
    21361 actttgaagg ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat
    21421 ctctatacca aaacggaaga cggtaaacag tggctggcaa cgcacgttga tggtctgccc
    21481 taatcgctga ttcgcagagg tccctatgtt cacagcaaca gaggcagtcc cggtagcaaa
    21541 agtggtggca ggaaataagc gttatcccgg cgtcgttgcg ctggataacg ttaacttcac
    21601 gctcaataaa ggcgaagttc gtgcgctgtt aggcaaaaac ggtgcgggaa aatcgaccct
    21661 cattcgaatg cttaccggta gcgaacgtcc ggatagcggt gatatctgga ttggcgagac
    21721 gcgactggaa ggtgacgaag ctacgctgac tcgccgtgcc gctgaactgg gggtacgcgc
    21781 ggtttatcag gagttaagtc tggtggaagg gctgacagtg gcggaaaacc tctgcctcgg
    21841 tcagtggccc cgccgcaacg gcatgattga ttacctgcaa atggcgcagg acgcccaacg
    21901 ttgcttgcag gcgttgggcg ttgacgttag tcctgaacaa cttgtttcaa cactaagccc
    21961 ggcgcaaaag cagctggtgg aaattgcgcg ggtgatgaag ggcgagccgc gcgtggtcat
    22021 tcttgatgaa cccaccagct cgcttgccag tgcggaggtt gaactggtga tcagcgcggt
    22081 gaaaaagatg tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat
    22141 tcgccgcatt gcctcctgtg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat
    22201 gctcgaaaac acctccacgc atcatattgt gtcgctaatg ctcgggcgcg atcacgttga
    22261 tattgcgccg gtttcccctc aggaaattgt cgatcaggcc gtgctggaag tccgtgcgtt
    22321 acgccataag cccaagctgg aggatatcag ctttacgctc cgtcgtggcg aagtactcgg
    22381 cattgctggc ctgctggggg cagggcgcag tgaattgttg aaagccatag ttgggctgga
    22441 gacgtatgaa cagggcgaaa ttgttatcaa cggcgagaaa atcactcgcc ccgattacgg
    22501 cgacatgctg aaacgcggca ttggctatac gccagaaaac cgcaaagatg cggggatcat
    22561 tccctggctg ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcgccaa
    22621 cggtgtgctg caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac
    22681 ggtcaaggcc gccagtagcg aaacgcccat cggcacgctt tctggcggca atcagcaaaa
    22741 agtggtgatc ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac
    22801 gcgtggcgtc gatatcgaag ccaaacagca gatttaccgt attgtccgcg agctggctgc
    22861 cgaaggaaaa agcgtggtgt ttatctccag tgaagtggag gagttgccgc tggtgtgcga
    22921 ccgcattctg ttgttacagc acggtacgtt ctcgcaggag tttcacgctc cggtcaatgt
    22981 ggatgagctg atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa
    23041 gatgtctgct tcgtcattac tattgccgca gggcaagagc gtctcgctca aacaatttgt
    23101 cagtcgccat attaatgaga tcggtttgct ggtggtgatc gccattcttt atctggtctt
    23161 ctctctgaac gcgcctggct ttatctcatt gaataaccag atgaacgtgc tgcgcgatgc
    23221 cgccaccatc gggatcgccg catgggcgat gacgctgatt attatctccg gtgaaattga
    23281 tgtcagcgtt ggtccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt
    23341 tgaagttccg ctggcggtgg cgtgtctgct ggtgttgctg ttaggcgcgc tgatgggaac
    23401 gctcgccggg gtgctgcgcg gcgtgtttaa cgtgccaagt tttgttgcca cgctgggttt
    23461 atggagcgcc ctgcgcggaa tggggctgtt tatgaccaac gccttgccag tgccgattga
    23521 tgaaaacgaa gtgctggact ggctgggggg acaatttctc ggtgtgccgg tatccgcgct
    23581 gatcatgatg gtattgtttg cgctgtttgt gttcatcagc cgcaaaaccg ccttcgggcg
    23641 ctcggttttt gccgttggcg gtaatgccac tgcagcacag ttgtgcggta ttaacgttcg
    23701 tcgggtacgc attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt
    23761 gttggcggcg cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt
    23821 catcgccgcg gtcgtggttg gcggtacggc gctttccggt ggtcgcggct cgttgttcgg
    23881 tacgctgctt ggcgtactgg tgattacgct aatcggtaac ggtctggtgc tgctcgggat
    23941 taactccttt ttccagcagg tggtgcgcgg cgtcatcatc gtggtggcgg tgctggcgaa
    24001 tatcttgctg actcagcgta gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg
    24061 ggtgcggcag ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg
    24121 gcagcttatt taccaggaaa ttcgaccgtc gatctgcggg aagttgcggt gccgacgccg
    24181 gggattaacc aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac
    24241 tatatctatc atcaacaccg tgccacggca gcggcacccg ataaaccgtt ataccaggga
    24301 tttatcaacg gtcatgaacc gtgcgggcag attgtggcga tggggcaagg ctgccgccat
    24361 tttaaagagg gcgaccgtgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac
    24421 tgccgtcgcg gctttcctat ctcttgtact ggcgaaggaa aagcggctta cggctggcag
    24481 cgtgacggcg gtcatgccga atatctgctg gcggaagaaa aagatctgat cctcctgccg
    24541 gatgcactga gttacgaaga tggtgcgttt atcagttgcg gcgttggtac agcgtatgaa
    24601 gggattttgc gcggcgaagt ttccggcagt gataacgtac tggtggtcgg tctggggccg
    24661 gtcggcatga tggcgatgat gctggcgaaa ggtcgcggtg caaaaaggat catcggcgtt
    24721 gatatgctgc cggaacgtct ggcgatggcg aaacagttag gagtgatgga tcacggctat
    24781 ttagcgacca ccgaaggtct gccgcagata atcgccgaac tcactcacgg cggcgcggat
    24841 gttgcgcttg attgttccgg taatgccgca ggtcgcttac tggcactgca atccaccgct
    24901 gactggggac gcgtggttta cattggtgaa accggaaaag tcgagttcga ggttagcgcc
    24961 gacctgatgc accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg
    25021 gaaaaatgcg cccacgattt aacggactgg aaactgtggc cgcgtaacgc cattacccac
    25081 cgcttctcgc tggaacaggc gggagatgct tatgcactga tggcaagcgg caaatgcggg
    25141 aaagttgtga ttaacttccc ggattaagga gttagcggat gaccatctat accttatccc
    25201 acgggtcgct aaagctgggc gtttctgacc agggcggcgt aatcgaaggc ttctggcgcg
    25261 atacgacgcc gctcttacgt cctggtaaaa aaaacggtgt ggcaaccgat gcctcttgtt
    25321 ttccgctggt gccatttgca aaccgggtga gcggcaaccg ttttgtctgg caggggcgtg
    25381 agtatcaact gcaaccgaat gttgagtggg atgcgcacta tctgcacggc gacggctggc
    25441 tggggcagtg gcaatgtgtc tcgcgcaatg atgatatctt gtgtctggtg tatgaacatc
    25501 gcagcggtgt ttatcactat cgggtaagcc agacgtttca tttaacggcg gatacgctga
    25561 cggtgacact ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc
    25621 atccttattt tccgttgtcg ccgcaaacgc ggattcaggc acaggcgagc ggttactggc
    25681 tggagcggga gcagtggctg gcgggtgagt tttgcgagca gctaccgcaa gaattggatt
    25741 ttaaccagct cgcgccgttg ccgcgccagt gggtgaacaa tggttttgcc ggatggaatg
    25801 gtcaggcccg catcgagcag ccgcaggagg gttatgccat catcatagaa acgacgccac
    25861 ctgcaccgtg ttatttcatc tttgtttccg acccggcgtt tgataaagga tatgcgtttg
    25921 atttcttctg tctggaaccg atgagccatg cgccagatga ccatcatcgc ccggaaggtg
    25981 gtgacctcat tgcgcttgca ccaggggaat caacaacttc agagatgtcg ttgcgggttg
    26041 cgttactgta atttataaaa tgccggataa ggcgttcaca ccgcatccgg cactcggcgc
    26101 acgatgcctg atgcgacgct tgccgcgtct tatcaggcct acaattggtg ttggaacggt
    26161 aggcaggata aggcgttcac gccgcatccg gcatctttcc taacgccgat cgagagaaat
    26221 cgcccccggc ccggtaattg ccaacagcag gaatgcgcca gcgatactga cattcttcca
    26281 gaaattaatc atatttggcc caaccgcatc gccggtcata tcccagtaat ggtgaccaat
    26341 caccgccgta cccagcgtgt agaaaataaa cagcaccgcc agcggacggg tgaaaaagcc
    26401 aagcacgatt aatatcgcgg cgggcacttc cataactacc gcaataatcg ctgccagcat
    26461 cggcatcggc gcgcccagcg aggccatata ttggaccgta ccgtcaaagc ccatcatttt
    26521 gggaaaacca aaaataatga aaattaagac cacggcgata cgggcaatta ataacaaaac
    26581 ggggcgtgca gcaccaaaat caaaataacg tagtgagttc ataaccaatc cctttgcaag
    26641 ataatgtgat ttaaacgtaa tacacactct tgcacctcgc cacgcccctg gcattgagat
    26701 attcgctaat tatcctggtt acagtgattt aagcgcgatg tcctcatcta tcagcagttt
    26761 cgcatcaaac gttttgccgc tctggatcca tttgcggatt aagcgaatct cacgcccctg
    26821 attcagcgtt accgcaccga taagcacgcc gttttgcaga ttaaaccaaa tcgccttctg
    26881 agtttccggg ttgccacgac aaatccagtc atcgccatgc atatcgccaa taaactgtaa
    26941 gttatcactg tactgatcgc tccagaacca cggcggcggc agtcgcggaa gcggtagccc
    27001 caacattgcg gcagcggcaa tttgcgcctg gttattggcg ttttcccagc tttcgcagcg
    27061 gtgtagtgca ccattatcaa tacgggtgat tgccacatcg ccaccggcaa agatcgcggg
    27121 atcgcaggtg cggcaagctt catcaatgac aataccattg gcagtatcaa ggttggcctc
    27181 gcgagccagt tggtcgttgg cgctgatacc aataccgtaa atcaccacat cggcccgaag
    27241 cgtttcgcca ctttgcagcg tcagttctac tttttcacca tcgaccacat gttcaatggc
    27301 attattgagc agaatgcgca caccagcctg ctgatggcgc ggtaaaagat agcgttgcac
    27361 gggcggtggt gcattacggc ccatgacgct tgccgccagt tcaatcactg tcaccttaca
    27421 tccacgctgc gtggcgctgg cagccagttc cagaccaata gttccggcac cgacaatcac
    27481 gactgaccgt tcgggctgca gaacttctcg cagtctggcg gcatcaccgg catggcgcag
    27541 ggtaaagcag cgttctccca gtgcatcaag caacggcagc ggtcgagctg ccgcgccggt
    27601 tgctataaaa agctgatccc agtgccagct ttcgccgtta gctaacacta actcttgtgt
    27661 gtcgcggccc aatgttttga tggttacacc ggaatgcaga tgaacattgt tttcctgcca
    27721 ccagtgagcg ggtaacacag actgcaactg tggggaatct tccagcaaca tggatttcga
    27781 gagcgggggg cgttcataag gaagatgttg ctcatcggaa aacagatgca gctcaccgct
    27841 gaacccttgc tggcgtagca aggccgcagc cattgccgcc gcttgcccgc caccgacaat
    27901 aatgatcgtt ttttctttca tcgtgcttcc ttaaagatcc agcccagccg ctacgtggcg
    27961 aatgccgcga atcgctaaac ccgcatcagc gttgatcatc acaccgctta atgcgcgatt
    28021 attgcgccgc gatgccaaca tcacatacgg acccgtaaaa tccgccggtt gcgggaaaaa
    28081 ttgcagcggt aaaatggcgg caattttctc cggcgtcaga gactgcatta tcgaggtttc
    28141 actttgcccg agcgcctgtg ggccgcgcag gtcgctggcc ataccacacg ggccgacgcc
    28201 attcacccgc actttcggtg ccagttcata agccagttgg cgaataagtc cggttgcggc
    28261 atgtttactg gcggtgtaca gcgggccacc gccgccggga taccaggcgg cgtttgacag
    28321 tgtgaaaatc atgctgcctt cactggcgat taacgccgga gcgcaggctt ttgcgcccag
    28381 caggtaaccg agtacgttga cattaaacag ctcgtggaag ccggtttcga gcgtctctgc
    28441 gggagtgtta accagtgagg cattgtgatc ccagatgccc gcattgccga taaaacaatc
    28501 cagcttgcca gaacgagtca ggatctgatt aaccgcgcgt tgataatcgg catagcaggt
    28561 cacgttacct tccaccgcca gaatatgttc gccaaatcgc tgacgcagac tggcgacttt
    28621 tgccgccgac agttccagcg tggcaacctg cgcgccttct tcgataaatc gctcggccag
    28681 cgccaggcct aatcccgatc cgccgccggt aataaagatg gactcgttat gcagatcgct
    28741 catggctgcg cctccggtaa gtcgatgaag atgtcaccac cttcaacgtg tactggataa
    28801 gtggttagcg gatcggtggc gggcaggcat aacgctttcc ccgttttcag gcaaaaactg
    28861 gcggcgtgca gcgggcactc caccgtggcg tcatcttcca gatacccttc tgacatcgac
    28921 gcattaccat ggctgcaacg atcgttaatg gcataaaact cgccgcctac gttgaacagg
    28981 gcgatgacgg gcgaggtatc gatccggaga gcctcaccct ccggcacatc cgcaacggga
    29041 cacgcataaa ttcgattcat cagaacagta cactcaggtt atgggaggag atgaccgctt
    29101 gatccaggac gatatcccgt tccagcaagc gccagttccc gtcctccaga cggcgaactt
    29161 tgtcgaaacg cgttccaaca tagaatgttt catcgcgctc tttttgtgcc cgataaagta
    29221 gataattcac cctcacggca aatacgtttg ggatgtcggt ttcgcttacc tggcagttgc
    29281 tgattaagtg acgagtgcgt gacggcggtt cttctgccca ggccatgccg gtttccagac
    29341 gggcgattcg ccgttccagc tggtctttgg tgtcattaaa aatccaggtt gtcggcggtt
    29401 gaacgccttt gcggcggtcg cgcgtttgtg cgttaaccgt ggtgcgcatg gtgtaacgaa
    29461 tctcttcgtc gagctgtgcc agccagtcac gaaatttcca gtcatccagc agactggctt
    29521 cgtgaaagag aaactggcta atgcggtgat gtaactctag tgaaacttgc gcactcattt
    29581 catcacctcc tgctggtaag cggcggtttt atcgagcact tcctgccagc tttcgctact
    29641 cagaagatcg gcccagcgtt ggtacattcc acgagcggcc gtttctgaga agatatagtt
    29701 agtaatgcct ggaatgccgt cgtcgcgacg cttttcctga ccaagcccca tttccagaca
    29761 cagtttgctg ttgcgggcgc ggtggccttt cagcaatttc tggatttcac accagttctc
    29821 cgagtcatcc tgctcgagaa aaccagcagg accaaaagca cgagtggcgc tgttttcaaa
    29881 agcggcttta acttcatcgg aggcggcttt gtcagtaata cagaacgccc acacttcgac
    29941 ttgatcaggg ccgcgcggat gccagacgcg gagcgtggca gtgccgttga gccatgaaag
    30001 cgtggggaaa atattgttat gacccgccag gcgcagggcg cgaacttcac ctaaacgttg
    30061 ttctgcttcg gcataggttt cgcggtaata gctggaaacc gcgccatcga cccagacatt
    30121 agcatccggt ttttcagtaa agaagaaacc gctaccgtga ccgtcctgac caaattgcag
    30181 cgcatctttg gcggtttccc acaccgggcg ggcggtttgt ccatcaccga gacgcttatc
    30241 gctgccatca tctttcgccc ctaatacctg aacggcagaa gcatggctga acagagcatg
    30301 atattggtca ctggcgaact gctctgccgg gaatttccag ttgcagttga tcacccactt
    30361 ttgtacaccg ccgacaattt cggtgccgcc ttcgcgacga tccagcatgc catccagata
    30421 ccaggcaatg tcacccaggt aatcacgcag gcccggtgcg ctggtatccc agttgccaaa
    30481 aatcagccct ttataactct ccacacaagg aacttcgttt agtccccagt gggatttaca
    30541 caacccttgt gggtaggcgc gaggttccag cggtacatcg atcaactcgc cgttaatgcc
    30601 ataagaccag ccgtgatacg gacaggtaaa ggcgcgagtg ttaccgcaat ctgcataact
    30661 cacacgcatg gcccggtggc ggcattggtt gagaaacgcc ttaatgctgc cgtctttctg
    30721 acgcactacg acaaccgcat cttcccccat gtaggtgtta aagaaatcac catgttttgg
    30781 gatctggctt tcgtgggcga gaaatagcca gcaacgaccg aaaatgcgct caagctccag
    30841 ttggtaaata tccgggtcgg tataaatacg cggagtgacc cgaccgtttt gggtgtcaat
    30901 cagttggtaa atgttcaaat ctgagggtgt ggtcatattt tttaatcctg aaagataaga
    30961 tgtattgacc ttctattttt gtgccataag gatgttgctg gttggttgct gatatgtgaa
    31021 atagttaatt tctaccagct gagacgtttt ttcgataaca aggggaaggt gatggaacta
    31081 cggcatttac gctatttcgt cgcagtggcg caggcactga actttacccg tgcggcggaa
    31141 aaactgcata cctcacagcc ttcgttaagc agccagatcc gcgatcttga aaactgtgtc
    31201 ggtgttccgt tgctggtgag ggataagcgc aaagtcgcgt tgacggcggc gggagaatgt
    31261 tttctccagg acgcgctggc aatcctcgaa caagcggaaa atgccaaatt acgggcgcgg
    31321 aaaattgttc aggaagacag acaattaacc attggctttg tgccatcggc ggaagtgaat
    31381 ttactgccaa aagtattacc gatgtttcgt ctcagacagc cagacacctt aattgagctg
    31441 gtgagtttaa tcaccacgga acaggaagaa aaaattcgcc gtggtgaact cgacgtcggc
    31501 ttaatgcgcc atcctgttta tagcccggag attgattatc tggagctttt tgacgaacca
    31561 ttagtggtgg tgttaccggt tgatcaccct ttagcacatg aaaaagagat cactgccgcg
    31621 caactggatg gcgtgaattt cgtcagtact gatccagcgt attccggttc gcttgcgccg
    31681 atcgttaaag cgtggtttgc gcaagaaaac agccagccaa atatcgtcca ggtggcaacg
    31741 aatattctgg tgaccatgaa tctggtgggg atggggctgg gcgtcacttt gatacccggt
    31801 tatatgaata attttaatac cggccaggtg gtatttcgac ccattgccgg aaatgtgcct
    31861 gccattgctt tactgatggc gtggaagaaa ggggagatga agccggcatt acgcgatttc
    31921 atcgccattg tgcaggaacg tttggcaagc gtaacggcat aattacgcat tatttttgca
    31981 aaacgcgtcg cggatcgcag ttttttagtg cttgtgaact ggcgttacag acatcgggaa
    32041 gcaggctcga atattcttat ttttcaactt gcaccaacat tcgattaacc ccagactaac
    32101 gcctcctgac gggagggact catggttttg caatccacgc gctggttggc gctcggctat
    32161 ttcacatact tttttagtta cggcattttt ctacctttct ggagcgtttg gcttaagggg
    32221 attggtttaa cgccagaaac catcggcctg ttattggggg caggtctggt tgcccgtttt
    32281 ctcgggagct tgctcatcgc gccccgcgtc agcgatcctt ctcgcctgat ttccgccttg
    32341 cgcgtgctgg cactgctgac acttctcttt gctgtcgcct tctgggcggg ggcgcacgta
    32401 gcgtggctga tgctggtgat gattggcttt aacctctttt tctcaccgct ggtaccgttg
    32461 accgatgcac tggcgaatac gtggcaaaag cagttcccgc ttgattacgg caaagtgcga
    32521 ttgtggggct cggtggcgtt tgtcattggc tcgtcgctga cgggcaaact ggtcactatg
    32581 tttgattatc gggtgatcct cgcgctgttg acgttgggcg tggcatccat gctgctcggc
    32641 tttctcatcc gtccgacgat tcagccacaa ggggcaagcc gccagcagga gagcaccggt
    32701 tggtctgcgt ggttggcgtt ggttcgccag aactggcgct ttctggcctg cgtttgttta
    32761 ttgcaggggg cgcatgcggc ctattacggt tttagcgcca tttactggca ggctgctggc
    32821 tactcggcct cggcggtggg ctatttgtgg tcgctgggcg tggtggcgga agtcattatc
    32881 tttgcgctga gtaataaact tttccgccgt tgtagtgcac gcgatatgct gttgatctca
    32941 gcgatttgcg gcgtagtgcg ctggggcatt atgggagcaa ctacggcgtt gccgtggttg
    33001 atagtggtgc aaattctgca ttgtggcacc ttcacggtct gccacctggc cgccatgcgc
    33061 tatattgccg ctcgccaggg tagcgaagtc atccgtttac aggcggttta ctctgccgtc
    33121 gcgatgggcg gcagtattgc gatcatgacc gttttcgccg gtttcctgta tcaatatctg
    33181 ggccacggcg tgttctgggt gatggcgctg gtggcgcttc cggcaatgtt tttgcgcccg
    33241 aaagttgttc cctcatgctg attcgagcat tttgcggatc tgctcctgtt gatgtgttgt
    33301 caacgtcagg tccgtataga tcagcggtgg tgaaaagagc ggtaacggca tggtataagg
    33361 agcaataatc agtgccgcgc tgcgcggagc gcctgtctgc aaaaatgcct ttaccgacat
    33421 atgtttaata ttgagcggca gtaacgttag ttcgcgtagc tgctgctcaa tctgcgcttc
    33481 tcgctcgcta tcattcccgg tcagtagaat aatctgtttc tcatgcaggt cgttttcctg
    33541 cattagccag gcaccaaaaa tcaccgcgac cagaccgctt tcctcatcag aaagatggac
    33601 gccgtattca ctttcaaatc cggccagcgc cgcgcgggtg gtgcgcacca ggcgtggata
    33661 caggcgggcg aactcttccg gcagggtgtt atcaataccg atggcaaaca aactgcgatt
    33721 taacgcctgg gcgaggtggg tataaagctg atcgcataac ccctgttcat cataaaaacg
    33781 aacatttccc agctcacgaa aatggtttac cagacgtttg atagacttac gcagttgtct
    33841 gtcccgctga tgcgcatccc gcagtggatc gggaacccgc aacatggaaa aaagtaatgc
    33901 cataaacagt ggctcatcag gcggtacagg ctggagcgcc cgacgctgcc agtggcgtcc
    33961 aatttcctgc gctacctgaa attcaaggca ggattccgcc cagcgacgtt ggagcggatt
    34021 aaactgaggc gtaattccag cgtggtgttg cagcaaacaa tattgcagat acagacacag
    34081 gaaatgaata tcgcgcgttt caaagcgttt ttgcagccgt cgggagcaga gattcactaa
    34141 cgcttgtaga ttggtatcgt cgtaaaagtt acgcgcaatt ccgcgccgct tcagttcact
    34201 cttcagggcg ggggtgaaat ggctggtaat aaatgacgga cacagacgga aaccacgtcg
    34261 tagccagtga aataaacaca aacgttggtt aagcactgta ccttcaaccc ggtagctacc
    34321 gtcataacca gtagtcagtg tgagttgatg atagcgcagg atctcccgtc ctgtttcgct
    34381 gatatcaaga ctggcaatat catcatccac accattaagc tcgctgaaag ttgccgtggt
    34441 ggcggtcaac cccggctgaa agagcgtcag caagatctgg cagcggcgct ggggagccga
    34501 aaggacagat ggtggagcaa gcgtaggcat catctgtaga tctcccataa gcataattga
    34561 taagaatagc taaagtttgc tcagggaaat ggcgaaggga atgctaatca tcagaaatgt
    34621 tggcaagcgt cacagaattt ttctcatctt aaactgttct gaccagtgga ttagcatgtc
    34681 ttaaggtggt tttgtgaatg attatcgtgc tagcgatcat gggttagttc tgcggattca
    34741 acgcgctaag gataaaatcc accatctgtt cgcgataaac atcgccgtga cgatcggaaa
    34801 aagcatcaat gtgttcgcca tccgggatta ggatcaaccg ttttggctct tttgccaggc
    34861 tatacaactt ttcgctgtgc tgccatggga taacgtgatc agctttaccg tgaatcagta
    34921 aaagcgggat cgggctgacg ctggcgatgt aattttcgcc gctgtaactc tcatcaagta
    34981 agtagccact gccggggatc atctggttgg cgatggtcgc ataagaggca aatgtggagt
    35041 cgaggatcac cgcacgtatg ccttcacgat caccctgacc aataacatcc aaaatattcg
    35101 ccccgccaat actctgcccg aacagcacca gccgttgtgg gtttacatca ctgcgatggc
    35161 gcaccacatt gatggcactt tgcgtatcgt ccagcaatcc ggcctgggac ggcgtgcctt
    35221 ttgatttacc aaacccgcga taatcaaaca taaaaacgtt gaaattacgc tcgggtaacc
    35281 aactgaccag cggccagtgg gcggacatat ttccggcatt gccgtgagca tgaataatgg
    35341 tggcaatggc cttttcagcg gggccagttg cagaagggat aaaccaacca tgtaggcgag
    35401 tgccatcttt agcggtgaat tctactgatt cggctgacca gggatcgggg ccgtagattt
    35461 tgtcgtcagg gtaatagaaa aaattgatgg cgatgcgcgg tattaaataa atgcagaagg
    35521 caacaacaaa gagaataaac agaattttga gaacgcgttt gttcactggc agcgccataa
    35581 cctttccttg cgacttcttt atcatccatt aatcggggaa agatggtggg agagtggcga
    35641 aagaaagtaa agcggtgagg aagtatttcg gaatagcgcc tgaaaggcga gggcgggtga
    35701 gtgacatcac ccgccagagt cattaacgct tcagggcgtc gcttaactcg tcacgcatgt
    35761 tcgccagcat ggctttaaca acgcgcgggt taccagcaac gatgttaccg gtcagcatgt
    35821 agttatgacc accggtgaag tcgctgacga tgccgcccgc ttcacgaacc agcagctcgc
    35881 ctgccgcgaa atcccacgga cgcagaccga tttcaaagaa accgtcaacg cgacccgcag
    35941 caacgtaagc cagatccagc gccgcagaac cggtacgacg gaagtctgca cattcgttga
    36001 acagtttgcc gacgatgttg atgtaggtag tggcgtactg ttttgctttg aacgggaagc
    36061 cggtcgccag aatagtaccg tcgagatcgc gagcggtgct gccgcgcaga cggtagccgt
    36121 tcagctgtgc gccctgaccg cgagtggcgg tgaacagttc gttacgcata ggatcgtaaa
    36181 ccacagcaac ttcagtgcgg cctttgatac gaacggcgat agataccgcg aagtgcggca
    36241 gacgtttgat aaagttggta gtgccatcca gtggatcgat aacccattga acatcctgat
    36301 cagtaccttc aagttcaccg ctttcttcgg tgatgatggt gtgctgtggg taagatttac
    36361 gaatcgtgtc gataatcacc gcttcggcag ctttatctac gttggtcacg aagtcgttac
    36421 tgcctttctg gctcgcttct acagcgtccg gggtttcata gtttttggca attaaattac
    36481 ccgccttgcg cgctgcgcgc acggcgatgt tcagcatcgg atgcatcggt ctctctcact
    36541 ggatgttaaa gaacgggaaa acggcgcaga gtatagcagc gagatcggca tctgtcttcg
    36601 ttttatgata agatgcgtga ataatcttta gatgaagaaa gacaatgctg caaaatattc
    36661 gaattgtgct ggtggagacg tcacacaccg gcaatatggg gtctgttgcc cgtgccatga
    36721 aaacaatggg attaaccaat ctgtggctgg ttaatccact ggtgaaacct gactcccagg
    36781 cgattgccct ggcagcaggg gccagcgatg tgattggtaa tgctcacatc gtcgatacgc
    36841 tcgacgaagc gttagccggt tgtagcctgg tggtgggcac cagcgcacgc tcccgcacgc
    36901 tgccgtggcc gatgctcgac ccgcgcgaat gcggtctgaa aagcgtcgct gaagcggcaa
    36961 ataccccggt ggcactggtg tttggtcgcg agcgcgtcgg cttgaccaat gaagagctgc
    37021 agaaatgcca ttatcatgtc gcgattgcgg ctaacccgga atacagttcg ctgaacctgg
    37081 cgatggcggt tcaggttatt gcctatgaag tgcgcatggc ctggctggca actcaggaaa
    37141 atggcgagca ggtcgagcat gaagagacgc cgtatccgct ggtcgatgat ctggagcgtt
    37201 tttatggtca tctggaacaa acgctgctgg caaccggttt tattcgtgaa aaccatccgg
    37261 ggcaggtgat gaataaattg cgccgtctgt ttacccgtgc gcgcccggaa agccaggaat
    37321 tgaatatcct gcgcgggatt ctggcttcta tagagcagca gaataaaggt aacaaggccg
    37381 aataatagtg gttgcctgat gcgacgctta acgcgtctta tcaggcctgc agtgcacaga
    37441 gcccctgggc ggataaggcg ttcacgtcgc atccgacaac aggtacaaac gccacgataa
    37501 aaaaatggca ctgaaggtta aatacccgac taaatcagtc aagtaaatag ttgaccaatt
    37561 tactcgggaa tgtcagactt gaccctgcta tgcaataccc ccacttttac aataaaaaac
    37621 cccgggcagg ggcgagtttg aggtgaagta agacatgaga ctgacatcta aagggcgcta
    37681 tgccgtgacc gcaatgcttg acgttgcgct caactctgaa gcgggcccgg taccgttggc
    37741 tgatatttcc gaacgtcagg gaatttccct ttcttatctg gaacaactgt tttcccgtct
    37801 gcgtaaaaat ggtctggttt ccagcgtacg tggaccaggc ggtggttatc tgttaggcaa
    37861 agatgccagc agcatcgccg ttggcgaagt gattagcgcc gttgacgaat ctgtagatgc
    37921 cacccgttgt cagggtaaag gcggctgcca gggcggcgat aaatgcctga cccacgcgct
    37981 gtggcgtgat ttgagcgacc gtcttaccgg ttttctcaat aacattactt taggcgaact
    38041 ggttaataac caggaagtgc tggatgtgtc tggtcgtcag catactcacg acgcgccacg
    38101 cacccgcaca caagacgcga tcgacgttaa gttacgcgct taataaaaag aattcagaat
    38161 caggccggag tgctaaacac tccgtaaaca cggtcgtaca tccagccggt agcctggttc
    38221 cttgcattga gtgatgtacg gagtttatag agcaatgaaa ttaccgattt atctcgacta
    38281 ctccgcaacc acgccggtgg acccgcgtgt tgccgagaaa atgatgcagt ttatgacgat
    38341 ggacggaacc tttggtaacc cggcctcccg ttctcaccgt ttcggctggc aggctgaaga
    38401 agcggttgat atcgcccgta atcagattgc cgatctggtc ggcgctgacc cgcgtgaaat
    38461 cgtctttacc tctggtgcaa ccgaatctga caacctggcg atcaaaggtg cagccaactt
    38521 ttatcagaaa aaaggcaagc acatcatcac cagtaaaacc gaacataaag cggtactgga
    38581 tacctgccgt cagctggagc gcgaaggttt tgaagtcact tacctggcac cgcagcgtaa
    38641 tggcattatc gacctgaaag aactcgaagc agcgatgcgt gacgacacca tccttgtttc
    38701 catcatgcac gtaaataacg aaatcggcgt ggtgcaggat atcgcggcta tcggcgaaat
    38761 gtgccgtgct cgtggcatta tctatcacgt tgatgcaacc cagagcgtgg gcaaactgcc
    38821 tatcgatctg agccagttga aagttgacct gatgtctttc tccggtcaca aaatctatgg
    38881 cccgaaaggt attggtgcgc tgtatgtgcg tcgtaaaccg cgcgtacgta tcgaagcaca
    38941 aatgcatggc ggcggtcacg agcgcggtat gcgttccggc actctgcctg ttcaccagat
    39001 cgtcggtatg ggcgaagcct atcgcatcgc aaaagaagag atggcgaccg agatggaacg
    39061 tctgcgcggc ctgcgtaacc gtctgtggaa cggcatcaaa gatatcgaag aagtttacct
    39121 gaacggtgac ctggaacacg gtgcgccgaa cattctcaac gtcagcttca actacgttga
    39181 aggtgagtcg ctgattatgg cgctgaaaga cctcgcggtt tcttcaggtt ccgcctgtac
    39241 gtcagcaagc ctcgaaccgt cctacgtgct gcgcgcgctg gggctgaacg acgagctggc
    39301 acatagctct atccgtttct ctttaggtcg ttttactact gaagaagaga tcgactacac
    39361 catcgagtta gttcgtaaat ccatcggtcg tctgcgtgac ctttctccgc tgtgggaaat
    39421 gtacaagcag ggcgtggatc tgaacagcat cgaatgggct catcattaat cggtatcgga
    39481 atcaggagaa tttataatgg cttacagcga aaaagttatc gaccattacg agaatccgcg
    39541 taacgtgggt tcctttgaca acaacgacga gaacgtcggc agcggcatgg tgggtgcacc
    39601 ggcctgtggc gacgtgatga agttgcagat taaagtcaac gatgaaggta tcattgaaga
    39661 cgcgcgtttt aaaacttacg gctgcggttc tgctatcgct tccagctccc tggtcaccga
    39721 atgggtgaaa gggaagtctc tcgacgaagc gcaggcgatc aaaaacaccg atattgctga
    39781 agaacttgaa ctgccgccgg tgaaaattca ctgttctatt ctggcagaag acgcgatcaa
    39841 agccgccatt gcggactata aaagcaaacg tgaagcaaaa taagagttga ggtttggtta
    39901 tgtcgattac actgagcgac agtgcagcag cgcgagtaaa tacctttctg gctaaccgcg
    39961 gcaaagggtt tggcctgcgt ctgggcgtga gaacctccgg atgttcaggt atggcttatg
    40021 tactggaatt tgttgacgaa ccgacgccgg aagacatcgt gtttgaagac aaaggcgtga
    40081 aagtcgtggt cgatggcaaa agcctgcaat ttctggacgg tacgcagctg gacttcgtaa
    40141 aagaaggcct gaacgaaggg tttaaattca ccaacccgaa cgtcaaagat gagtgtggtt
    40201 gcggcgaaag cttccacgtt tgatgcgcat accgataacc ccaccgtggt cgcctgcgcg
    40261 tggggtttgt tttacctgat tcgccgttat cgcggcggat cgcagccctg agaatgttat
    40321 ggattacttc accctctttg gcttgcctgc ctgttatcaa ctcgataccc aggcgctgag
    40381 cctgcgtttt caggatctac aacgtcagta tcatcctgat aaattcgcca gcggaagcca
    40441 ggcggaacaa ctcgccgccg tacagcaatc tgcaaccatt aaccaggcct ggcaaacgct
    40501 gcgtcatccg ttaatgcgcg cggaatattt gctttctttg cacggttttg atcttgccag
    40561 cgagcagcat actgtgcgcg acaccgcgtt cctgatggaa cagctggaac tgcgcgaaga
    40621 actggacgag atcgaacagg cgaaagatga agcgcggctg gaaagcttta tcaaacgtgt
    40681 gaaaaagatg tttgataccc gccatcagtt gatggttgaa cagttagaca acgagacgtg
    40741 ggacgcggcg gcggataccg tgcgtaagct gcgttttctc gataaactgc gaagcagtgc
    40801 cgaacaactc gaagaaaaac tgctcgattt ttaatttctg gaagctaaac atggccttat
    40861 tacaaattag tgaacctggt ttgagtgctg cgccgcatca gcgtcgtctg gcggccggta
    40921 tcgaccttgg cacaaccaac tcgctggtgg cgacagtgcg cagcggtcag gccgaaacgt
    40981 tagccgacca tgaaggccgt cacctgctgc cgtctgttgt tcactatcaa cagcaagggc
    41041 attcggtggg ttatgatgcg cgtactaacg cagcgctcga taccgccaac accattagtt
    41101 ctgttaaacg cctgatgggg cgctcgctgg ctgatatcca gcaacgctat ccacatctgc
    41161 cttatcaatt ccaggccagc gaaaacggcc tgccgatgat tgaaacggcg gcggggctgc
    41221 tgaacccggt gcgcgtttct gcggacatcc tcaaagcact ggcggcgcgg gcaactgaag
    41281 ccctggcagg cgagctggat ggtgtagtta tcaccgttcc ggcgtacttt gacgatgccc
    41341 agcgtcaggg caccaaagac gcggcgcgtc tggcggggtt gcatgttctg cgcttactta
    41401 acgaaccgac cgctgcggct atcgcctacg ggctggattc cggtcaggaa ggcgtgatcg
    41461 ccgtttatga cctcggcggc gggacgtttg atatttccat tctgcgctta agtcgcggcg
    41521 tgtttgaagt gctggcgacc ggcggtgatt ccgcgctcgg cggcgatgat ttcgaccatc
    41581 tgctggcgga ttacattcgc gagcaggcgg gcattcctga tcgtagcgat aaccgcgttc
    41641 agcgtgaact gctggatgcc gccattgcag ccaaaatcgc gctgagcgat gcggactccg
    41701 tgactgtgaa tgttgcgggc tggcagggcg aaatcagccg tgaacaattc aacgaactga
    41761 tcgcgccact ggtaaaacga accttgctgg cttgtcgtcg cgcgctgaaa gacgctggcg
    41821 tagaagctga tgaagtgctg gaagtggtga tggttggcgg ttctactcgc gtgccgctag
    41881 tgcgtgaacg agtaggcgaa tttttcggtc gtccaccgct gacttctatc gacccggata
    41941 aagtcgtcgc tattggcgcg gcgattcagg cggatattct ggtgggtaac aagccggaca
    42001 gcgaaatgct gttgctggat gtgatcccgc tgtcgctggg cctcgaaacg atgggcggcc
    42061 tggtggagaa agtgattccg cgtaatacca ctattccggt ggcgcgcgct caggatttca
    42121 ccacctttaa agatggtcag acggcgatgt ctatccatgt aatgcagggt gagcgcgaac
    42181 tggtgcagga ctgccgctcg ctggcgcgtt ttgcgctgcg tggtattccg gcgttaccgg
    42241 ctggcggcgc gcatattcgc gtgacgttcc aggtcgatgc cgacggtctt ttgagcgtga
    42301 cggcgatgga gaaatccacc ggcgttgagg catctattca ggtcaaaccg tcctacggtc
    42361 tgaccgacag cgaaatcgcc tcgatgatca aagactcaat gagctatgcc gagcaggacg
    42421 taaaagcccg tatgctggca gaacaaaaag tagaagcggc gcgtgtgctg gaaagtttgc
    42481 acggcgcgct ggctgctgat gccgcgctgt taagcaccgc agagcgtcag gtcattgacg
    42541 atgctgccgc tcacctgagt gaagtggcgc agggcgatga tgttgacgcc atcgaacaag
    42601 cgattaaaaa cgtagacaaa caaacccagg atttcgccgc tcgccgcatg gaccagtcgg
    42661 ttcgtcgtgc gctgaaaggc cattccgtgg acgaggttta atatgccaaa gattgttatt
    42721 ttgcctcatc aggatctctg ccctgatggc gctgttctgg aagcgaatag tggtgaaacc
    42781 attctcgacg ttgcgctgcg taacggtatc gagattgaac acgcctgtga aaaatcctgt
    42841 gcttgcacca cctgccactg catcgttcgc gaaggttttg actcgctgcc ggaaagctca
    42901 gagcaggaag acgacatgct ggacaaagcc tggggactgg agccggaaag ccgtttaagc
    42961 tgccaggcgc gcgttaccga cgaagattta gtggtcgaaa tcccgcgtta cactatcaac
    43021 catgcgcgtg agcattaaca gaggttagta tgggacttaa gtggaccgat agccgcgaaa
    43081 ttggcgaagc actgtacgat gcgtatcccg atcttgatcc gaaaacggtt cgattcaccg
    43141 atatgcatca gtggatttgc gatctggaag atttcgacga cgacccgcag gcatcgaacg
    43201 agaaaatcct cgaagcgatt ttgttggtct ggctggacga agccgagtga atgaacgggc
    43261 tgcctggggg tagcccgttt tgctgttaaa cgaataagga taactaaaat gacagaagcg
    43321 atgaaaatta ccctttctac ccaacctgcc gacgcgcgct ggggagaaaa agcgacttac
    43381 agcattaata atgatggcat taccctgcat ttgaacggga cagacgatct ggggctgatc
    43441 cagcgtgcgg cgcgcaagat tgacggtctg ggcatcaagc atgttcagtt aagcggtgaa
    43501 gactgggatg cggatcgctg ctgggcattc tggcaaggtt acaaagcccc gaaaggcaca
    43561 cgtaaagtgg agtggccgga tctggacgat gcccaacggc aggaactgga taaccgcctg
    43621 atgatcatcg actgggtgcg tgacaccatc aacgcaccgg cggaagaact ggggccatcg
    43681 caactggcac agcgtgctgt tgatctgatc agcaacgtcg cgggcgatcg tgtgacttac
    43741 cggatcacca aaggcgaaga tctgcgtgat caaggttata tggggctgca cacggtcggg
    43801 cgcggttcag aacgttcccc ggtattgctg gcgctggatt acaacccaac aggcgataaa
    43861 gaagcgccag tgtacgcgtg cctggttggt aaaggcatca cctttgattc cggcggctac
    43921 agtatcaaac agaccgcatt tatggactcg atgaagtcgg acatgggcgg cgcggcaacg
    43981 gttaccggag cgctggcgtt tgccattacg cgcggactga acaagcgcgt gaagctgttc
    44041 ctctgctgtg cggataacct gatcagcggc aatgcgttca agctgggcga tatcattacc
    44101 tatcgcaacg gtaaaaaagt tgaagtgatg aacaccgatg cggaagggcg tctggtgctg
    44161 gccgatggcc tgattgatgc cagtgcgcag aaaccggaac tgatcattga tgcggcgacc
    44221 ctcaccgggg cggcgaaaac cgcgctgggt aatgattatc acgcgctgtt cagttttgac
    44281 gatgcgcttg ccgctcgctt gctggcgagt gccgcgcagg agaatgaacc attctggcgt
    44341 ctgccgctgg cggagttcca ccgcagccag ctgccgtcta actttgccga actgaacaat
    44401 accggaagcg cggcgtatcc ggcaggtgca agcacggcgg cgggcttcct gtcgcacttt
    44461 gttgagaact atcagcaagg ctggctgcat atcgactgct cggcgactta ccgtaaagcg
    44521 ccggttgaac agtggtctgc gggcgcgacc gggctaggtg tgcgcacgat tgctaatctg
    44581 ttaacggcgt aaacgtctta tttggcctac aaaattgcga aaatccaata gattgagatt
    44641 cccttgtagg cctgataagc gtagcgcatc aggcacttgt gccccgaacg ttgaatgaat
    44701 tgtggttctt atgtccgaaa caaaaaacga acttgaagac ctgctggaaa aagcagcaac
    44761 cgaaccggcg caccgcccgg cctttttccg tactctactg gaatccaccg tctgggtgcc
    44821 gggtacggcg gcgcagggcg aggctgtggt tgaagatagc gcgcttgatt tacagcactg
    44881 ggaaaaagaa gacggcacca gcgtcattcc tttttttacc tcgttagaag cacttcaaca
    44941 ggcggttgaa gacgaacagg catttgtcgt aatgcccgtt cgcacattgt ttgagatgac
    45001 ccttggcgaa acgctcttcc ttaatgccaa actgccaacc ggtaaagaat ttatgccgcg
    45061 tgaaatcagt ttgctgattg gtgaagaagg aaatccgctg agcagccagg aagtactgga
    45121 aggcggtgaa tcgctgatat tatcggaagt cgcagagcca ccagcacaaa tgattgattc
    45181 actcaccacc ttatttaaaa ctattaagcc ggtgaagcgt gcttttattt gttcaattaa
    45241 agagaacgaa gaggcacagc ctaatttact tattggcatt gaagccgatg gtgatatcga
    45301 agaaattatt caggcggcgg gaagtgtagc gaccgataca ttacctggcg atgaacccat
    45361 cgatatttgt caggtgaaaa aaggggaaaa aggaattagc cactttatta ccgaacatat
    45421 tgcgccattc tatgaacgtc gctggggtgg ttttttgcgt gactttaaac agaaccggat
    45481 aatctaaaat atgccgcccc aaagggcggc attcgggctt acttgctgct ctgaaggttg
    45541 ttaaccgcca gtaaattgtt gccggagaga ctccggttat tatttacctc gcttaacagc
    45601 gcaccagaaa caataagtac gatacaaaag caccgtatct tcattgtgcg gtccatgcga
    45661 agaccgcatc acgacagcac cactggcgat attgccgcga tagcaagcgc tgagacgcgc
    45721 ttgctctaac ggtcgcggaa catcctttcc ttaagccagc gcccgcttgc gagacggcgg
    45781 aaaggtccgc agtggataat acactaacag aacaataata actctctttt acttgacaaa
    45841 aagagagtta ctggcgagta ttgtgtccgc tgagacgcgc ttgcattact acggtaatac
    45901 aatctgctgg aaaggccgcg aaccaaacca gcaacaaata accgcctgat tttggcggtt
    45961 ttttgttttt taaaggaaag tgaatttaca tgtttaagta atatctttta ccggttagta
    46021 tttttaattt tgagcaggat ttgtttatat ttgtctgaat tcggactaat aagccaggtt
    46081 attttattac cgaacacggc ggaaagggcc ggtgtcgata atacgctaaa taaacaatga
    46141 ttactctctt tcgcttgaca aaaagagagt tactggcgag tattgctttg ctgtgaagca
    46201 cctgcgttgc tcatgcaaca caagccaatc tgctggaaag gccgcgaacc agaccagcaa
    46261 taaaaaaccg ccagattcgg cggtttttta ttgcctgtta aaagcttatt tctctggctc
    46321 aactggtaaa tctgctcgtg cgccccattc actccatgcg ccgtcgtaca gtttcacgtt
    46381 tggcacatca agtgtcgcga gtgccaacaa aaccacggct gccgttacac cagagccgca
    46441 gctgacgata ataggtttgt cataacttac gccgcgacca aaaaatatcg catccagttc
    46501 atcggtcgtt tttagttcac cttcgcgcac cagttccgtc cacggaacat ttagtgcacc
    46561 gggaatatgt ccgcgacgta aacctgggcg aggttcatca acttctgcgt taaaacgtgc
    46621 agccgggcgg gcatcgataa tttgcgccgt attttcatgg cttgccaata acacatcggt
    46681 tactttcacc acggcttcag gattaaacgc ggcgttaaac tctccttccg gcagctctac
    46741 tgcgccttct tctaatagca gatcatcacg ctgccagcct gcaagtccac cacccagaat
    46801 cgacactttc tctacaccaa aggtgcgcag catccaccat gctcgtgggg ctgagaaaag
    46861 attaccttcg tcatagacaa tcaggtgctt atcctggtta acgcctaatt cacgcatcgc
    46921 cacggcgaac gtttccgggc gcggcagcat gtgcggaagc ggggaagtgt gatcagaaag
    46981 cgcttctata tcaaaaaaca ctgcgccggg aatatgtcca ttcagatact cctgagcaac
    47041 gttacgatct tcctgtccag gcgacgccat gcgggcatcg ataatctgaa tttccgggtc
    47101 atcaatatgt tcggcgagcc agtcggctcc tacaaaccat gtcgtggaca taggcatctc
    47161 catttttgcc ggggttaggc taaattgtcg gatgtttagt gaaaggcgac aagcaaaaga
    47221 ggctgatttg gctgtctgag ccacaattct cactaaaatt cggattgtat gggcgtgata
    47281 ctgaattaca taacgtaaat tctcacgcat aataattatt cgacgttgct aacgggccat
    47341 tgtggcaggg atgaaaaatg aaaaagttac gcgtagccgc ctgcatgcta atgctggcgc
    47401 tggcagggtg cgacaacaac gataacgcgc caacagcggt gaaaaaagat gcgccttctg
    47461 aagttactaa agcggcctct tcagaaaacg tgagttcagc aaaactttcc gcgccagagc
    47521 gacaaaaact ggcccaacag agtgccggta aggcgctgac attgctggat ctctctgaag
    47581 ttcaacttga tggcgcagcc acgctggtgc tgacattctc catccctctc gacccggatc
    47641 aggaattctc acgcgttatc catgtcgtcg ataagaaaag cggcaaagtg gatggtgcct
    47701 gggagctgtc agataatctt aaagagttgc gtttacgcca cctcgaaccg aaacgtgatt
    47761 tgatcgtcac tattggcaag gaggtcaaag cactcaacaa cgcaaccttc agtaaagatt
    47821 acgaaaaaac tataactacc cgagatatcc aacccagcgt cggttttgcc agccgtggct
    47881 cgttgttacc tggtaaagtt attgaagggc tgccggtaat ggcgctcaac gttaacaatg
    47941 tcgatgttaa cttcttccgt gttaagccag aatctctgcc tgcattcatt agccaatggg
    48001 aataccgcaa ttcgctggcg aactggcagt ctgacaaact gctgcagatg gcggatctgg
    48061 tttataccgg acggtttgat ctcaattctg cgcgtaacac ccgtgaaaaa ttattgctgc
    48121 cgctgggcga tatcaaaccg cttcagcagg caggcgtgta tctggctgtg atgaatcagg
    48181 ctggacgtta cgattacagt aatcccgcga cgctgtttac gttaagtgat atcggcgttt
    48241 cagctcaccg ttatcacaat cgtctggata tctttaccca aagcctggaa aacggcgcgg
    48301 cccagcaagg agttgaagtc tcattattaa atgagaaagg gcagactctg actcaggcaa
    48361 ccagtgatgc ccaggggcat gtgcagctgg aaaatgataa aaacgcggca ttactgttgg
    48421 cgcgtaaaga cggtcagaca acgctactcg atttaaaact tccggcgctg gacttagcag
    48481 aatttaacat tgctggcgcg ccaggctata gcaaacagtt tttcatgttt ggcccgcgcg
    48541 atctttatcg ccctggtgaa acagtcatcc tcaatggttt attgcgtgat gcagatggta
    48601 aagcattgcc cgatcaaccc atcaagttag acgtgattaa acccgacggg caggtactca
    48661 ggagcgtcgt tagtcagccg gagaatggcc tctaccactt tacctggcca ctcgatagca
    48721 atgcggcaac cggtatgtgg catattcgcg ctaacacggg cgataaccag tatcggatgt
    48781 gggatttcca cgtcgaagat tttatgccag agcgcatggc gctgaatctg accggtgaga
    48841 aaaccccgct aacgccgaaa gatgaagtga gattctccgt ggtggggtac tacctgtatg
    48901 gtgcgcctgc taatggtaat actttgcaag ggcaactttt cctgcgccca ctgcgtgaag
    48961 ctgtgtcagc cttacctggt ttcgaattcg gcgatatagc tgccgaaaat ctttcccgca
    49021 cgctggatga agttcagttg acgctggatg ataaagggcg tggcgaagtt tctacagaaa
    49081 gccagtggaa ggaaacgcat tccccattac aggttatttt ccagggtagt ttgttggaat
    49141 cgggcggtcg cccggtgacg cgccgcgctg agcaggctat ctggcctgcc gatgcattgc
    49201 cggggatccg tccgcagttc gcctcgaaat cggtttacga ttatcgcact gacagcacgg
    49261 tgaaacagcc cattgttgat gaaggcagta acgccgcttt tgacatcgtt tatagcgatg
    49321 cgcaaggcgt aaaaaaagcc gtgtcgggct tgcaggtgcg cctgattcgc gaacgccgcg
    49381 attactactg gaactggtca gaagatgaag gctggcagtc acagtttgat caaaaagatc
    49441 tgatcgaaaa tgaacaaact ctggatctga aagcggacga aaccggcaag gtcagtttcc
    49501 cggtagagtg gggcgcttat cgtctggaag tcaaagcgcc gaatgaagcg gtcagtagtg
    49561 tgcgtttctg ggctggctat agctggcagg acaacagcga cgggagcggt gccgtgcgac
    49621 ccgaccgtgt cacgctgaaa ctggataaag ccagctatcg ccctggcgat accattaagt
    49681 tgcatattgc cgcgccaacg gcgggtaaag gttatgcgat ggtcgagtcc agtgaagggc
    49741 cgctgtggtg gcaagagatt gatgttccgg ctcaagggct ggatctgacg attccggtcg
    49801 ataaaacctg gaatcgtcat gatctgtatt taagtacgct ggtggtacgt cctggcgata
    49861 aatctcgctc cgcgacgcca aaacgcgcgg ttggggtgtt gcatctgccg cttggtgatg
    49921 aaaaccgtcg cctcgatctg gcgctggaaa cccctaccaa aatgcgcccc aatcagccat
    49981 taaccgtgaa aattaaagcc agcaataaaa atggtgagat gccaaaacag gtgaatgtgc
    50041 tggtgtctgc cgttgatagc ggtgtgctga atattactga ttacgtcacg ccagatccgt
    50101 ggcaggcgtt ctttggtcag aaacgctatg gcgcagacat ttacgatatt tacggtcagg
    50161 tcattgaagg tcaggggcgt ctggcagctc tgcgttttgg tggtgatggt gatgagctga
    50221 aacgtggtgg taaaccgccg gtcaatcacg tcaatattgt cgcgcagcag gcgctgccgg
    50281 taacgctcaa cgaacagggc gaaggctcgg ttacactgcc aattggcgat tttaacggtg
    50341 aattgcgcgt catggcgcaa gcctggacgg cagatgactt cggaagcaac gaaagcaaag
    50401 tgattgttgc tgcaccggtg attgctgaac tgaacatgcc acgctttatg gcgagtggcg
    50461 atacctcgcg cctgacgctg gatatcacta atcttaccga taaaccgcaa aaacttaatg
    50521 ttgccctgac cgccagtggt ttgcttgaac tggtcagcga ttcacccgca gccgttgaat
    50581 tagcgccagg ggtgcgtact acgctgttta tcccggtgcg tgcattgccg ggttatggcg
    50641 atggagacat tcaggccacc attagcgggt tagcgttacc cggggaaacc gttgccgatc
    50701 agcataagca gtggaaaatc ggcgtccgtc cggcattccc ggcacaaacg gtcaattacg
    50761 gtacggcgtt acagcctggt gaaacctggg cgctcccggc tgatggattg caaaacttct
    50821 cgcctgttac gctggaaggg caattgttgt tgagcggcaa accaccgctg aacatcgcac
    50881 gttatatcaa agagttaaaa gcgtatccgt acggctgtct cgagcaaacc accagcggcc
    50941 tgttcccgtc actttatacc aatgcagccc aactgcaggc gttgggcatc aaaggcgaca
    51001 gtgatgagaa acgccgtgca tcggtcgata tcggcatttc ccgtttgctg caaatgcaac
    51061 gtgataacgg cggctttacg ctgtgggata aaaacggtga cgaagagtac tggctgacgg
    51121 cttacgtgat ggatttcctg gtccgcgcag gcgaacaggg ttacagcgta ccgacagacg
    51181 ccattaaccg gggtaatgag cgtctgctgc gctatttaca agatccgggc atgatgtcga
    51241 tcccgtacgc tgataatctc aaagccagta aattcgccgt acagtcttac gctgcgctgg
    51301 tgttggcccg tcagcaaaaa gcaccactgg gtgcgctgcg tgaaatctgg gagcatcgtg
    51361 cagatgccgc ttctggttta ccgctgctgc aacttggcgt tgcgctgaaa accatgggtg
    51421 atgcaatgcg tggtgaagaa gcgattgtgc tggcgctgaa aacgccgcgt aatagtgatg
    51481 agcggatatg gctgggtgat tacggtagtc cactgcgcga cagcgcgttg atgctctcat
    51541 tgctggaaga gaacaaactg ctacccgatg agcagtactc cctgttgaac acactttcgc
    51601 agcaggcgtt cggtgaacgc tggctatcga cgcaggaaag taacgcgttg ttcctggctg
    51661 cccgtactct tcaggattta ccgggcaaat ggcaggcgca aaccactttc tctgctgagc
    51721 cgctgacagg cgagaaagcg caaaccagca atctgaatag cgatcaactt gccaccttgc
    51781 aggtgaccaa cagtggcgat cagccgttat ggctgcgtgt ggatgccagc ggttatccgc
    51841 aatccgcacc tttatcggcg agcaatgtgc tgcaaattga gcgtcatatt ctgggtactg
    51901 acggtaagag caaatcgctg gactcgttac gtagcggcga tctggtgctg gtgtggttgc
    51961 aggttaaagc cagtaacagt gtaccggatg cgttagtcgt ggatctgttg cctgcggggc
    52021 tggaactgga aaaccagaat ctggcgaacg gcagcgccag cctggagcaa agtggtggtg
    52081 aagtgcagaa cttgctgaac cagatgcagc aggcgagcat taagcacata gagttccgtg
    52141 acgatcgctt tgtggcggcg gttgccgttg atgaatatca accggtaacg ctggtgtatc
    52201 tggcgcgggc ggtgacgccg ggaacgtatc aggtgccgca gcctatggtg gaatcaatgt
    52261 atgttcctca atggcgggcg actggtgcgg ctgacgatct gttgatagtc agaccgtaaa
    52321 tgcctcgcct gttaaccaaa cgcggctgct ggataatgct ggcagccgcg ccttttatca
    52381 tcattctggc agcatgggca gcagataaac tctggcctct gccattgcaa gaagtgaatc
    52441 ctgcacgcgt ggtggtggcg caggatggta cgccactctg gcgtttcgcc gatgctgacg
    52501 gtatctggcg ttatccggta acaatcgaag atgtttctcc acgttacctt gaagcgctga
    52561 tcaattatga agatcgctgg ttctggatgc atccgggggt gaatccgttc tcggtggcgc
    52621 gtgcagcctg gcaagatctc acgtcgggtc gggtcatttc cggcggcagc acgctcacca
    52681 tgcaggtggc gcgcctgctt gatcctcacc ccaaaacgtt tggcggcaaa attcgccagc
    52741 tctggcgcgc gttgcaactg gaatggcatc tgtctaaacg tgaaattctg accttgtatc
    52801 ttaaccgtgc cccgtttggc ggtacgttgc aggggattgg tgcggcaagt tgggcttacc
    52861 tcgggaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg gttttgccgc
    52921 aagcgcccag ccgtcttcgc ccggatcgtt ggccggagcg tgccgaagcc gcgcgtaata
    52981 aagtgctcga acggatggcc gtgcaaggtg tgtggtcccg tgagcgggta aaagagtcaa
    53041 gggaagaacc catctggctg gcaccccgac aaatgccgca actggcactg ctgttttcgc
    53101 gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat gccggtcttc
    53161 aacgacgtct ggaagaactg gcgcaaaact ggaaaggacg attgccaccg cgcagctcac
    53221 tggcgatgat cgtggttgat cataccgata tgcgtgttcg cggctgggtg ggatcagttg
    53281 atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc cgatcgccag
    53341 gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg atccacccgg
    53401 catcgctgtt gcaagacgtc cccagacgca ccggtgatta ccgacctggc aactttgaca
    53461 gcggttttca tggcccgatc agcatgagtg aggcgctggt gcgatcgctg aacttacctg
    53521 ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta cgcaatgttg
    53581 gattgccgtt atatttgccc aacggtgctg caccgaatct ttcgctcatt ctcggcggcg
    53641 ctggcgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc cacggcaagg
    53701 caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta atgtcgtcgg
    53761 gggctgcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg ccggatggtg
    53821 ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc tatcgtgatg
    53881 cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc agaccggacg
    53941 gcacgcccgt tgttggtcag tttggctttg ccagtgccat accgctgttg aatcaggtta
    54001 ataatatctt actgtcgcgc agtgtgaatt tgccggaaga cccacgtcct gactctgtaa
    54061 gtcgtggcgt tatctgctgg ccgggcggac agtctttggc ggaaggtgac ggtaactgtc
    54121 gccgccgcct ggcaacctgg ctgctggacg ggagccagcc gccaactcta ttactgccgg
    54181 agcaggaagg cattaatggc attcgtttcc ccatctggct ggatgaaaac ggcaaacgtg
    54241 ttgccgctga ttgccctcag gcgcgacaag aaatgataaa tgtctggccg ctaccgctgg
    54301 aaccctggct gcccgcatca gaacgtcgcg ctgtgcgctt gccaccggcc tcgacaattt
    54361 gcccgccata cggtcacgat gcacaactcc cgctgcagct aacaggtgtg cgcgatggcg
    54421 cgattattaa acgtttaccg ggcgcagcgg aagcgacttt gccgttgcaa tcaagtggag
    54481 gggcaggtga acgctggtgg tttctgaacg gcgaaccgtt aactgaacgt gggcgcaacg
    54541 tgaccctgca tttgacggat aaaggcgatt atcaattgct ggtgatggat gaggtggggc
    54601 aaatagcgac agtgaaattt gtcatgcaat agtctgtttt gtccctgatt gttgcttaaa
    54661 ctgggtttat tttaaaaaaa tgttaccttc atcaatagtc aacggctctg ttgctcatta
    54721 taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac agaggtaaaa
    54781 atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa aaacgtgatt
    54841 ggtagtatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac caaaatgctg
    54901 cacctgaccg ttgaacaggc tcgtggcttt tatgctgaac acgatggaaa accgttcttt
    54961 gatggtctgg ttgaattcat gacttctggc ccgatcgttg tttccgtgct ggaaggtgaa
    55021 aatgctgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa cgcactggcc
    55081 ggtactctgc gtgctgatta cgctgacagc ctgaccgaaa atggtaccca cggttctgat
    55141 tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga agtgtgcccg
    55201 cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg cgccagaatt
    55261 tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt ttttcaaccc
    55321 tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa caattagtca
    55381 cacctgaaaa cgtcaccact aaagatggaa aaatcaacct gctggatctc aaccgtcagc
    55441 agatgcggga gttttttaaa gatttaggtg aaaaaccctt ccgcgccgat caggtgatga
    55501 agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc aacaaagtgt
    55561 tgcgcggcaa actgaaagag gtggcggaga tccgcgcacc ggaagtggtt gaagaacagc
    55621 gttcatctga cggcaccatt aaatgggcaa ttgccgttgg cgatcagcgc gtcgaaacgg
    55681 tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg gggtgtgcgc
    55741 tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg cgggtatcgg
    55801 aaattatcgg ccaggtgtgg cgtgcggcga aaattgtcgg cgcagcaaaa gtcaccggtc
    55861 agcgcccaat cactaacgtc gtgatgatgg gtatgggcga gccgctgctc aacctgaaca
    55921 acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg tcaaaacgtc
    55981 gcgtcacgct ttccacttcc ggtgtggttc cggcgctgga taaactgggc gatatgatcg
    56041 acgttgcgct ggctatctcc ctgcacgcgc cgaacgacga aattcgtgac gaaattgtgc
    56101 cgatcaacaa aaagtacaat atcgaaacgt tccttgcggc ggtgcgccgt tatctggaga
    56161 aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac cacgttaacg
    56221 acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg tgtaagatca
    56281 acctgatccc gtggaacccg ttcccgggcg cgccgtatgg acgcagttcg aacagccgta
    56341 tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg cgtaaaacgc
    56401 gtggtgatga cattgatgct gcctgtggtc agttggcggg cgatgttatc gaccgtacga
    56461 aacgtaccct gcgtaaacga atgcagggtg aagccatcga cattaaagcg gtctgatagc
    56521 cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga agtattaagt
    56581 tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca gtatgtaacg
    56641 atgcaacagt aagttagcct tagcgaatgt gggctttgtc acgagcacac agacggtctt
    56701 atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtggg catgatggtt
    56761 caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg aagccacgca
    56821 cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg aacaactagg
    56881 acttagtcaa caggccgttg ccgagcgact ttgcctgaag gtttccacgg tacgcgacat
    56941 tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat atatccgctc
    57001 ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg aaaagcaggc
    57061 tccactacgg gctgcaaaag ttgcgccgat gcagagtttt tccctcggta aacgccgcaa
    57121 aaaacgtgac ggctggctga tgaccttcac ctggctggta ttgtttgtgg ttattggcct
    57181 gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca ccactatggc
    57241 cgatcaatct tctgcggaac tgaataataa tcaaagccag agtgtaccgt tagatacgtc
    57301 gacaaccaca gatcaggcta tggcgaccac gccgacatca cctgtggata ccaccgcaac
    57361 caacacgcaa acacctgccg taactgcgcc agcaccagct gttgatccgc aacagaatgc
    57421 ggttgttcca ccttcgcagg caaatgttga taccgctgcg actccggcac cagcggcaac
    57481 aacaacacca gatggcgctg cgccgttgcc aaccgatcag gctggcgtga ccacgccggc
    57541 ggttgacccg aatgcgctgg tgatgaactt tactgccgat tgctggctgg aagtcaccga
    57601 tgctaccggt aaaaaactgt ttagcggtat gcagcgtaaa gacggtaatt tgaacttaac
    57661 cggccaggca ccgtacaaac tgaaaattgg tgcgccagcc gcagtacaga tccagtatca
    57721 agggaaacct gtcgatctga gtcgttttat cagaactaac caggttgcgc gtctgaccct
    57781 caatgccgaa caatcaccgg cgcagtaaca gacgggtaac gcgggagatt tttcatgcat
    57841 aaccaggctc caattcaacg tagaaaatca acacgtattt acgttgggaa tgtgccgatt
    57901 ggcgatggtg ctcccatcgc cgtacagtcc atgaccaata cgcgcacgac agacgtcgaa
    57961 gcaacggtca atcaaatcaa ggcgctggaa cgtgttggcg ctgatatcgt ccgtgtctcc
    58021 gtaccgacga tggacgcggc agaagcgttc aaactcatca aacagcaggt taacgtgccg
    58081 ctggtggctg acatccactt cgactatcgc attgcgctga aagtagcgga atacggcgtc
    58141 gattgtctgc gtattaaccc tggcaatatc ggtaatgaag agcgtattcg catggtggtt
    58201 gactgtgcgc gcgataaaaa cattccgatc cgtattggcg ttaatgccgg atcgttggaa
    58261 aaagatctgc aagaaaagta tggcgaaccg acgccgcagg cgttgctgga atctgccatg
    58321 cgtcatgttg atcatctcga tcgcctgaac ttcgatcagt tcaaagtcag cgtgaaagcg
    58381 tctgacgtct tcctcgctgt tgagtcttat cgtttgctgg caaaacaaat cgatcagccg
    58441 ctgcatctgg ggatcaccga agccggtggt gcgcgtatcg gggcagtaaa atccgctatt
    58501 ggtttaggtc tgctgctgtc tgaaggcatc ggcgacacgc tgcgcgtatc gctggcggcc
    58561 gatccggtcg aagagatcaa agtcggtttc gatattttga aatcgctgcg tatccgttcg
    58621 cgcgggatca acttcatcgc ctgcccgaca tgttcgcgtc aggaatttga tgttatcggt
    58681 acggttaacg cgctggagca acgcctggaa gatatcatca ctccgatgga cgtttcgatt
    58741 atcggctgcg tggtgaatgg cccaggtgag gcgctggttt ctacactcgg cgtcactggc
    58801 ggcaacaaga aaagcggcct ctatgaagat ggcgtgcgca aagaccgtct ggacaacaac
    58861 gatatgatcg atcagctgga agcgcgcatt cgtgcgaaag ccagtcagct ggacgaagcg
    58921 cgtcgaattg acgttcagca ggttgaaaaa taataacgtg atgggaagcg cctcgcttcc
    58981 cgtgtatgat tgaacccgca tggctcccga aacattgagg gaagcgttga gggttcattt
    59041 ttatattcag aaagagaata aacgtggcaa aaaacattca agccattcgc ggcatgaacg
    59101 attacctgcc tggcgaaacg gccatctggc agcgcattga aggcacactg aaaaacgtgc
    59161 tcggcagcta cggttacagt gaaatccgct tgccgattgt agagcagacc ccgctattca
    59221 aacgtgcgat tggtgaagtc accgacgtgg ttgaaaaaga gatgtacacc tttgaggatc
    59281 gcaatggcga cagcctgact ctgcgccctg aagggacggc gggctgtgta cgcgccggca
    59341 tcgagcatgg tcttctgtac aatcaggaac agcgtctgtg gtatatcggg cctatgttcc
    59401 gtcacgagcg tccgcagaaa gggcgttatc gtcagttcca tcagttgggc tgcgaagttt
    59461 tcggtctgca aggtccggat atcgacgccg aactgattat gctcactgcc cgctggtggc
    59521 gcacgctggg tatttccgag cacgtaactc ttgagctgaa ctctatcggt tcgctggaag
    59581 ctcgtgcgaa ttaccgcgat gcgctggtgg cattccttga gcagcataaa gaaaagctgg
    59641 acgaagactg caaacgccgc atgtacacta acccgttgcg cgtactcgat tcaaaaaatc
    59701 cggaagtgca ggtgcttctc aacgacgctc cggcattagg cgattatctg gacgaggaat
    59761 ctcgtgagca ctttgccggt ctgtgcaaac tgcttgagag cgcgggtatc gcttacaccg
    59821 taaaccagcg tctggtgcgc ggtctggatt actataaccg taccgttttc gagtgggtga
    59881 ctaacagcct cggttcccag ggcaccgtgt gtgcaggtgg tcgttatgac ggtctggtgg
    59941 aacaactggg tggtcgtgca acaccggcag tcggttttgc gatgggcctc gaacgtcttg
    60001 tattgttagt tcaggccgtt aatccggaat ttaaagccga tcctgttgtc gatatatacc
    60061 tggtggcttc aggtgctgac acacaatctg cggctatggc attagcagag cgtctgcgtg
    60121 atgaattacc gggcgtgaaa ctgatgacca accacggcgg cggcaacttt aagaaacagt
    60181 ttgcccgtgc tgataaatgg ggtgcccgcg ttgctgtggt gctgggtgag tctgaagtgg
    60241 ctaacggcac agcagtagtg aaggatttgc gctctggtga gcaaactgca gttgcgcagg
    60301 atagcgtagc cgcgcatttg cgcacgttac tgggttaagg aaggagaagg acagcgtgga
    60361 aatttacgag aacgaaaacg accaggttga agcggttaaa cgcttttttg ctgaaaatgg
    60421 caaagcactg gctgttgggg tgattttggg cgttggcgca ctgattggct ggcgctactg
    60481 gaatagccat caggttgatt ctgcacgctc cgcttctctt gcctatcaaa atgcggttac
    60541 cgcagtgagc gaaggcaaac cggatagcat cccagcggcg gaaaaatttg ctgctgaaaa
    60601 taaaaatact tatggtgcgc tggcttcttt ggaacttgcg cagcaatttg ttgacaaaaa
    60661 tgaactagag aaagctgccg cccagttaca acaggggctg gcagacacga gcgatgaaaa
    60721 tctcaaagcc gtgataaatc tgcgtcttgc tcgcgttcag gtacagctca agcaggctga
    60781 tgccgcgcta aaaacccttg atgctatcaa aggtgaaggg tgggctgcca ttgttgccga
    60841 cctgcgtggt gaagcattgc tgagcaaagg tgataagcaa ggtgcgcgta gtgcatggga
    60901 agcaggcgtg aaaagcgatg ttaccccggc actgagcgaa atgatgcaga tgaaaattaa
    60961 taatttgtcc atctgagagg gacccgatgc aattgcgtaa attactgctg ccaggactgc
    61021 tttccgttac ccttttaagc ggctgttcgc tgtttaacag cgaagaagat gtggtaaaga
    61081 tgtccccatt gccaaccgtt gaaaaccagt ttacgccgac tactgcgtgg agcacttccg
    61141 ttggtagcgg cattggcaac ttctattcca atcttcatcc ggcactggcg gacaacgttg
    61201 tctatgcagc ggaccgcgct ggtttagtaa aagcgctgaa tgcggatgac ggcaaagaaa
    61261 tctggtctgt caacctggcc gagaaagatg gctggttctc taaagatcct gcattacttt
    61321 ctggcggcgt gaccgtgtct ggtgggcatg tctacattgg cagcgaaaag gcgcaggttt
    61381 acgcgctgaa taccagcgat ggtactgtgg catggcaaac taaagtcgcg ggtgaagcac
    61441 tttcgcgccc ggtggtcagc gacggtctgg tgttaatcca caccagtaac ggtcagttac
    61501 aagcgctgaa cgaagctgac ggcgctgtca aatggacagt taacctcgat atgccgtcgc
    61561 tctctttgcg tggcgagtct gcgccggcaa cggcttttgg tgcggccgtc gtgggtggcg
    61621 ataatggtcg cgtcagcgca gtgctgatgg aacagggcca gatgatttgg cagcagcgta
    61681 tttcccaggc gaccggttct accgaaattg accgtctgag cgatgttgac acgactcccg
    61741 tcgttgttaa cggcgttgtt ttcgcgctgg cctataatgg taacctgacg gcgcttgatc
    61801 tgcgcagcgg tcagattatg tggaaacgcg aactgggttc ggtgaatgat ttcatcgtcg
    61861 acggcaaccg catctatctg gtcgatcaaa atgaccgggt gatggcgttg accattgatg
    61921 gcggcgttac gctgtggaca caaagcgatc tgctgcatcg cctgctgact tctccggtgc
    61981 tgtataatgg caacctggtg gtaggtgaca gtgaaggtta tctgcactgg attaacgtcg
    62041 aagatggtcg tttcgttgcc cagcaaaaag ttgatagttc cggtttccag actgaaccgg
    62101 ttgctgctga cggcaaactg ctgatccagg caaaagacgg aaccgtgtac tctattacac
    62161 gttaatcgtc tctgtcgttc actttgaaaa cggctcctgg acaggggccg ttttcctgtt
    62221 tttaacaacg acacgaatat agcgtgcgtt gtctgatgat ttataaaaat gaggctttaa
    62281 acatggtacc tgtggtcgcg cttgtcgggc gccctaacgt aggaaaatcc acgttattta
    62341 accgtctaac tcgcacccga gatgcgctgg ttgcggattt cccgggtctg actcgtgacc
    62401 gtaagtacgg tcgtgcggaa attgaaggcc gcgagtttat ctgcattgat accggcggga
    62461 ttgatggcac cgaagacggt gtagagacac gtatggcgga acagtcgctg ctggcgattg
    62521 aagaagcgga cgttgtactg tttatggtgg atgcgcgcgc gggcctgatg ccggcggatg
    62581 aagcgattgc taaacatctg cgctcccgtg aaaaaccgac cttcctggtg gcaaacaaaa
    62641 ctgacggtct ggatcccgat caggcagtgg ttgatttcta cgcgcttggt ttaggtgaaa
    62701 tctacccgat cgccgcgtct cacggtcgtg gcgtattaag tctgctggag cacgtgctgc
    62761 tgccgtggat ggaagatctc gcaccgcaag aagaagtcga cgaagacgcc gaatactggg
    62821 cgcaatttga agcggaagag aacggcgaag aagaagaggc agacgacttc gacccgcaaa
    62881 gtctgccgat caaactggcg attgtgggtc gtccgaacgt aggtaagtct acactcacta
    62941 accgtattct tggtgaagag cgcgttgttg tttacgacat gcctggcacg acgcgtgaca
    63001 gtatctacat tccaatggaa cgcgacggac gtgagtatgt gctcattgac accgctggtg
    63061 tacgtaaacg cggcaaaatc accgatgcgg tagagaaatt ctcggtaatc aaaacgttgc
    63121 aggccattga agatgccaac gtggtgatgt tggtgattga tgcgcgtgaa ggtatttccg
    63181 atcaggatct ctccttgctg ggctttattc tcaatagtgg gcgctcactt gtgattgtag
    63241 tgaacaagtg ggacggcctg agccaggaag tgaaagagca ggtgaaagag acgctggact
    63301 tccgtctggg ctttatcgat tttgctcgtg tgcactttat ctctgccttg cacggcagtg
    63361 gtgttggtaa cttgtttgaa tcagtacgtg aagcgtatga cagctccacc cgtcgtgtgg
    63421 ggacctctat gctgacgcgc atcatgacga tggctgttga agatcaccaa ccaccgctgg
    63481 tacgcggtcg tcgtgtgaag ctgaaatacg cccacgccgg gggttataac ccgccgattg
    63541 tggtgattca cggcaatcag gtgaaagacc tgcctgattc ctacaagcgt tacctgatga
    63601 actacttccg caaatcgctg gacgtaatgg gatcgccgat tcgtattcag ttcaaagaag
    63661 gggaaaaccc gtatgcgaac aagcgtaaca ccctgacgcc aacccagatg cgtaaacgta
    63721 agcgtctgat gaagcatatc aagaaaagta agtaatcact tatctggttt ccctttccac
    63781 acggagaggg aagccgaata acacgcgagg agtatttatg gcactcaact gtccaacctg
    63841 caaaacccct ctcgaacgca atggcgagac tgctcactgt gcaacttgcg acaaagactt
    63901 taccgtccag gcactttgcc ctgagtgtca tcaggcgtta caagtgttaa aagcttgtgg
    63961 tgctgtggat tatttctgcc aacatgggca cggtttaata tctaaaaaac gcgttg
//
LOCUS       DAZGAP010000004        60081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGAP010000004 DAZGAP010000000
VERSION     DAZGAP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.004"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(65..517)
                     /gene="tabA"
                     /locus_tag="ACHLAM_000180"
     CDS             complement(65..517)
                     /gene="tabA"
                     /locus_tag="ACHLAM_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO7851773.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(662..1255)
                     /gene="bdcR"
                     /locus_tag="ACHLAM_000181"
     CDS             complement(662..1255)
                     /gene="bdcR"
                     /locus_tag="ACHLAM_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7851774.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILEE"
     gene            1326..2039
                     /gene="bdcA"
                     /locus_tag="ACHLAM_000182"
     CDS             1326..2039
                     /gene="bdcA"
                     /locus_tag="ACHLAM_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7851775.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2171..2566
                     /gene="yjgH"
                     /locus_tag="ACHLAM_000183"
     CDS             2171..2566
                     /gene="yjgH"
                     /locus_tag="ACHLAM_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7851776.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            2847..2981
                     /gene="pyrL"
                     /locus_tag="ACHLAM_000184"
     CDS             2847..2981
                     /gene="pyrL"
                     /locus_tag="ACHLAM_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO7851777.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2985..3920
                     /gene="pyrB"
                     /locus_tag="ACHLAM_000185"
     CDS             2985..3920
                     /gene="pyrB"
                     /locus_tag="ACHLAM_000185"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO7851778.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3933..4394
                     /gene="pyrI"
                     /locus_tag="ACHLAM_000186"
     CDS             3933..4394
                     /gene="pyrI"
                     /locus_tag="ACHLAM_000186"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO7851779.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4467..4853
                     /gene="ridA"
                     /locus_tag="ACHLAM_000187"
     CDS             4467..4853
                     /gene="ridA"
                     /locus_tag="ACHLAM_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO7851780.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5059..7755)
                     /gene="mgtA"
                     /locus_tag="ACHLAM_000188"
     CDS             complement(5059..7755)
                     /gene="mgtA"
                     /locus_tag="ACHLAM_000188"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO7851781.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGVYSRRYGWQ"
     gene            complement(7896..7949)
                     /gene="mgtL"
                     /locus_tag="ACHLAM_000189"
     CDS             complement(7896..7949)
                     /gene="mgtL"
                     /locus_tag="ACHLAM_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO7851782.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8134..9081
                     /gene="treR"
                     /locus_tag="ACHLAM_000190"
     CDS             8134..9081
                     /gene="treR"
                     /locus_tag="ACHLAM_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO7851783.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            9200..10621
                     /gene="treB"
                     /locus_tag="ACHLAM_000191"
     CDS             9200..10621
                     /gene="treB"
                     /locus_tag="ACHLAM_000191"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO7851784.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10671..12326
                     /gene="treC"
                     /locus_tag="ACHLAM_000192"
     CDS             10671..12326
                     /gene="treC"
                     /locus_tag="ACHLAM_000192"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO7851785.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGESETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRRLMTVGEMSSTSLEHCQRYATLTGSELSMTFN
                     FHHLKVDYPSGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            12711..14849
                     /gene="nrdD"
                     /locus_tag="ACHLAM_000193"
     CDS             12711..14849
                     /gene="nrdD"
                     /locus_tag="ACHLAM_000193"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO7851786.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15007..15471
                     /gene="nrdG"
                     /locus_tag="ACHLAM_000194"
     CDS             15007..15471
                     /gene="nrdG"
                     /locus_tag="ACHLAM_000194"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO7851787.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15516..15902)
                     /gene="cybC"
                     /locus_tag="ACHLAM_000195"
     CDS             complement(15516..15902)
                     /gene="cybC"
                     /locus_tag="ACHLAM_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO7851788.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16085..17437)
                     /gene="pmbA"
                     /locus_tag="ACHLAM_000196"
     CDS             complement(16085..17437)
                     /gene="pmbA"
                     /locus_tag="ACHLAM_000196"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO7851789.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17531..18082
                     /gene="yjgA"
                     /locus_tag="ACHLAM_000197"
     CDS             17531..18082
                     /gene="yjgA"
                     /locus_tag="ACHLAM_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO7851790.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18240..19613)
                     /gene="mpl"
                     /locus_tag="ACHLAM_000198"
     CDS             complement(18240..19613)
                     /gene="mpl"
                     /locus_tag="ACHLAM_000198"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO7851791.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19789..20787
                     /gene="fbp"
                     /locus_tag="ACHLAM_000199"
     CDS             19789..20787
                     /gene="fbp"
                     /locus_tag="ACHLAM_000199"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO7851792.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20820..21815)
                     /gene="yjfF"
                     /locus_tag="ACHLAM_000200"
     CDS             complement(20820..21815)
                     /gene="yjfF"
                     /locus_tag="ACHLAM_000200"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO7851793.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(21802..22824)
                     /gene="ytfT"
                     /locus_tag="ACHLAM_000201"
     CDS             complement(21802..22824)
                     /gene="ytfT"
                     /locus_tag="ACHLAM_000201"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO7851794.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(22838..24340)
                     /gene="ytfR"
                     /locus_tag="ACHLAM_000202"
     CDS             complement(22838..24340)
                     /gene="ytfR"
                     /locus_tag="ACHLAM_000202"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO7851795.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(24480..25436)
                     /gene="ytfQ"
                     /locus_tag="ACHLAM_000203"
     CDS             complement(24480..25436)
                     /gene="ytfQ"
                     /locus_tag="ACHLAM_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO7851796.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25746..26276
                     /gene="ppa"
                     /locus_tag="ACHLAM_000204"
     CDS             25746..26276
                     /gene="ppa"
                     /locus_tag="ACHLAM_000204"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO7851797.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26734..27729)
                     /locus_tag="ACHLAM_000205"
     CDS             complement(26734..27729)
                     /locus_tag="ACHLAM_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7851798.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(27796..28095)
                     /locus_tag="ACHLAM_000206"
     CDS             complement(27796..28095)
                     /locus_tag="ACHLAM_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7851799.1"
                     /translation="MSTNVGEKIRIMRKSEMLTQEKMAEITGLTVAALRQYEQGRNEP
                     NLESTKKLLKSPVFRKYRDWFLFDEVEPKAGQVVPALAHIGRSESESPHSGKKTG"
     gene            28203..28628
                     /locus_tag="ACHLAM_000207"
     CDS             28203..28628
                     /locus_tag="ACHLAM_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO7851800.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
     gene            28941..29279
                     /locus_tag="ACHLAM_000208"
     CDS             28941..29279
                     /locus_tag="ACHLAM_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO7851801.1"
                     /translation="MTQRRNFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRR"
     gene            29299..29541
                     /locus_tag="ACHLAM_000209"
     CDS             29299..29541
                     /locus_tag="ACHLAM_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO7851802.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIVEPYFTEVAA"
     gene            29538..29663
                     /locus_tag="ACHLAM_000210"
     CDS             29538..29663
                     /locus_tag="ACHLAM_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851803.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            29743..30021
                     /locus_tag="ACHLAM_000211"
     CDS             29743..30021
                     /locus_tag="ACHLAM_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851804.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTQKTLTEITNVLDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELRAQSGQFSA"
     gene            30033..30284
                     /locus_tag="ACHLAM_000212"
     CDS             30033..30284
                     /locus_tag="ACHLAM_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851805.1"
                     /translation="MNSIFTATRRTLLTHFTDAAGREFMVESYLITTTTPCPSDADYL
                     YIHLADGTQITAITSTVREVMTIRGAWKSETQAHGELRP"
     gene            30281..30493
                     /locus_tag="ACHLAM_000213"
     CDS             30281..30493
                     /locus_tag="ACHLAM_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851806.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            30520..31074
                     /locus_tag="ACHLAM_000214"
     CDS             30520..31074
                     /locus_tag="ACHLAM_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7851807.1"
                     /translation="MRLTPMGIPGKAPAHVKAWTQQEDRLLISLYQEHNTHQMAERIQ
                     RTRKAVMHRIRFLRNRGLIGRKRKAPLSTEAITFLIKNRHVKTAQEMALEVGCSECTV
                     RYTLRKRGYNLKKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEHINSTIQMHIN
                     DDAAFHLYHRRTATDAVLCELLPD"
     gene            31084..31464
                     /locus_tag="ACHLAM_000215"
                     /pseudo
     CDS             31084..31464
                     /locus_tag="ACHLAM_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31395..31622
                     /locus_tag="ACHLAM_000216"
     CDS             31395..31622
                     /locus_tag="ACHLAM_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO7851808.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVTFIMQSNALHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            31691..32107
                     /locus_tag="ACHLAM_000217"
     CDS             31691..32107
                     /locus_tag="ACHLAM_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851809.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMALVLEMTTHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            32104..34623
                     /locus_tag="ACHLAM_000218"
     CDS             32104..34623
                     /locus_tag="ACHLAM_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7851810.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVDFLTNTDEGKRINSHFHHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERTCDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTAPDPEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMACVRGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRKAINKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSPENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAALHRIRQFQFFGIPPRQV
                     WRELRRLAGQMARNPTAPQRLDHDDIDAILAAADVGCFATYITRQGGVLIPRNTYLVR
                     TAYETAEEANDYGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTNEGFD
                     VECGFAAPWTRGNNCPPDQNLTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTPTER
                     KQLTRQLKNPPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALGVQLHE
                     SAVVSVRHGARVRLADGRTAYWNDSTRRIDITPAPNTEAPQ"
     gene            34620..35024
                     /locus_tag="ACHLAM_000219"
     CDS             34620..35024
                     /locus_tag="ACHLAM_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851811.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDNTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLVTINRKAAIARYVHSAAGRQSRR"
     gene            35073..35243
                     /locus_tag="ACHLAM_000220"
     CDS             35073..35243
                     /locus_tag="ACHLAM_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851812.1"
                     /translation="MNDTRRISDRELTKMIESARQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            35240..35566
                     /locus_tag="ACHLAM_000221"
     CDS             35240..35566
                     /locus_tag="ACHLAM_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO7851813.1"
                     /translation="MKTPGMTGKEIILRYLETHTRFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(35632..35895)
                     /locus_tag="ACHLAM_000222"
     CDS             complement(35632..35895)
                     /locus_tag="ACHLAM_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO7851814.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(35933..36985)
                     /locus_tag="ACHLAM_000223"
     CDS             complement(35933..36985)
                     /locus_tag="ACHLAM_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7851815.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLCF
                     DVDAGHVAEEKAVPVG"
     gene            complement(36982..38787)
                     /locus_tag="ACHLAM_000224"
     CDS             complement(36982..38787)
                     /locus_tag="ACHLAM_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO7851816.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WATLLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGHEDGVAAGTCGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFAL
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPARRAVEFPKESAMRVGCECPDGIW
                     RYIIRLEEAVAGGLSARVDIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEV
                     EMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQ
                     GFNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNR
                     LVMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDV
                     TGHADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
     gene            38954..40003
                     /locus_tag="ACHLAM_000225"
     CDS             38954..40003
                     /locus_tag="ACHLAM_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO7851817.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     MLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTSPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAATGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTAAKA
                     KLEDAIKTFSTITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGITPR
                     PAGAPAESEKPFDFV"
     gene            40015..41034
                     /locus_tag="ACHLAM_000226"
     CDS             40015..41034
                     /locus_tag="ACHLAM_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO7851818.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGGENEFFNRIQNFVNRQIALDILRIAFNGTSMAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEEA
                     EDVGA"
     gene            41052..41750
                     /gene="gpM"
                     /locus_tag="ACHLAM_000227"
     CDS             41052..41750
                     /gene="gpM"
                     /locus_tag="ACHLAM_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO7851819.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNAA"
     gene            41858..42331
                     /locus_tag="ACHLAM_000228"
     CDS             41858..42331
                     /locus_tag="ACHLAM_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO7851820.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVAEVNTTLAPVVAHWKAQGHERAADVPGARLGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            42328..42801
                     /locus_tag="ACHLAM_000229"
     CDS             42328..42801
                     /locus_tag="ACHLAM_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7851821.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            42798..43508
                     /locus_tag="ACHLAM_000230"
     CDS             42798..43508
                     /locus_tag="ACHLAM_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851822.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQAAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQNGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITIMEDQIIAGPRQQKARTWTITLPSRVFLGVTNEEFSHILA
                     RQMNAMGYGLDVDAQDIR"
     gene            43520..44665
                     /locus_tag="ACHLAM_000231"
     CDS             43520..44665
                     /locus_tag="ACHLAM_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFO7851823.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRTLNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            44667..45128
                     /locus_tag="ACHLAM_000232"
     CDS             44667..45128
                     /locus_tag="ACHLAM_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFO7851824.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            45133..45438
                     /locus_tag="ACHLAM_000233"
     CDS             45133..45438
                     /locus_tag="ACHLAM_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO7851825.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            45428..46180
                     /locus_tag="ACHLAM_000234"
     CDS             45428..46180
                     /locus_tag="ACHLAM_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7851826.1"
                     /translation="MTHKPYISPFLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYLNDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            46180..46518
                     /locus_tag="ACHLAM_000235"
     CDS             46180..46518
                     /locus_tag="ACHLAM_000235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO7851827.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGEYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGVK"
     gene            46515..46901
                     /locus_tag="ACHLAM_000236"
     CDS             46515..46901
                     /locus_tag="ACHLAM_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851828.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDRR"
     gene            47001..47276
                     /locus_tag="ACHLAM_000237"
     CDS             47001..47276
                     /locus_tag="ACHLAM_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFO7851829.1"
                     /translation="MTTKSTGTITLTVAGVTLTFEPNKTAFNNLINEITMTNKIAPMT
                     TYLNRIVTADCRDALADLMDRYPGCEMQIAEKINEVYSPKLEIELKN"
     gene            complement(47240..47467)
                     /locus_tag="ACHLAM_000238"
     CDS             complement(47240..47467)
                     /locus_tag="ACHLAM_000238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851830.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            47466..49652
                     /locus_tag="ACHLAM_000239"
     CDS             47466..49652
                     /locus_tag="ACHLAM_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7851831.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKALGELEAKGVGSQALTALTTQAMKLGTAFGQSSTEI
                     IRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARFRSEFSAM
                     GAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAILGTLSRT
                     MGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAA
                     QQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVAS
                     FYNIRAAIGNALYPILAPLAGRFADIGATFVRWLNMFPNIARWLGYITLTMLALGAAG
                     AVTNIVLGVFSFIMEGHKAIIKGARAAWSLYTLALTKVRSAIIATSLAARLAGVSFLT
                     MSAPVLLVAAAIAALVVVAIKFWQPVKAFIRGFIDGFTGALDAMSPVTAAFGDIARAL
                     TPVWQGIKTLFGAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALEVVIS
                     VIRTLVDIFAIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTW
                     NWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETAVPALAGNRISAELPP
                     GGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
     gene            49649..49999
                     /locus_tag="ACHLAM_000240"
     CDS             49649..49999
                     /locus_tag="ACHLAM_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFO7851832.1"
                     /translation="MNTPSLYIDLLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            49977..51161
                     /locus_tag="ACHLAM_000241"
     CDS             49977..51161
                     /locus_tag="ACHLAM_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7851833.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILIIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     RGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMAVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGETSAPFIEAVNSYI
                     NEEGHHGHGDDLQCFAMPETRHRLEVTVYIRSSTTLPPDGAEGLKQDISDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLSKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            51154..51702
                     /locus_tag="ACHLAM_000242"
     CDS             51154..51702
                     /locus_tag="ACHLAM_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7851834.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAARAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTSLYRRRIAHAFVNAQDAGEVAGFIAIFE
                     RLGIGHVELQERKPGVDWDVILVQVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     NPFIIHYGAYEGEYICWTARAE"
     gene            51725..53791
                     /locus_tag="ACHLAM_000243"
     CDS             51725..53791
                     /locus_tag="ACHLAM_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7851835.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLASMDE
                     RQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLRAELSTHHPMTTPAFPSR
                     IWLNVWWTGTLTSEWATEHEIITADPDVTEERLRKTYPRDGIQQYFCAIAEIDEDGNV
                     TDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGYVGETGQRWTTEQFVEWL
                     NERGAFRPGLWIFKANISLSRHSVLTDTGYGDICLGGCLIEVSGDINGGIIRITTGLE
                     SPNPADMYLRTQFTGVVLNKGKSTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPDGYAIMVGQTFDKAQYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTDLGTKTTSSFDYGTKTTSTNGQHEHSVPLRRQDGGGINFDWLDG
                     SSSGSHIGNGYVPAAGDHQHTVPIGAHTHTLALGSHGHGLTINPTGNAENTVKNIAFN
                     YIVRLA"
     gene            53791..54387
                     /locus_tag="ACHLAM_000244"
     CDS             53791..54387
                     /locus_tag="ACHLAM_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7851836.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENAWHLMEDHRGKTVWRLESGSALLITEPGPLPENVTMIKP
                     DGEFCRWDGSSWVKDEAAEQAYRVSEAENKKRKLIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRTDTGNAPDIVWPESPSPDHHAG"
     gene            54399..55124
                     /locus_tag="ACHLAM_000245"
     CDS             54399..55124
                     /locus_tag="ACHLAM_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851837.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQARQLAAQRPDAAGLRRQIS
                     RFISRRAGALAGLEDGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTARP"
     gene            55096..55638
                     /locus_tag="ACHLAM_000246"
     CDS             55096..55638
                     /locus_tag="ACHLAM_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851838.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARATPGSSRKQTPQTPGAKGGTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            55641..57314
                     /locus_tag="ACHLAM_000247"
     CDS             55641..57314
                     /locus_tag="ACHLAM_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851839.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEAPTEAVSAGN
                     IADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFPPPGTLVEVG
                     FVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWERQTDQRIQET
                     SRERVVSADEEKRSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTSGHMM
                     VVAGTCERDVEGDTQDTVGGSLTEKIKGVRHSIAQAQKLVGDTVKLGNEGINVLTMLT
                     DLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            57731..58021
                     /locus_tag="ACHLAM_000248"
     CDS             57731..58021
                     /locus_tag="ACHLAM_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851840.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSDDE
                     NEKIRKARTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKTATSSVARNGRKR"
     gene            58178..58318
                     /locus_tag="ACHLAM_000249"
     CDS             58178..58318
                     /locus_tag="ACHLAM_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7851841.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHTSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            complement(58435..58632)
                     /locus_tag="ACHLAM_000250"
     CDS             complement(58435..58632)
                     /locus_tag="ACHLAM_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851842.1"
                     /translation="MILSRKKIEIEVVQSGTADNPETITKIKVSRPEDLEACGVIVEK
                     ICAIHAKSVNTTASNDHNKYN"
     gene            58902..59042
                     /locus_tag="ACHLAM_000251"
     CDS             58902..59042
                     /locus_tag="ACHLAM_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7851843.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSEFAAFLQC
                     KQ"
     gene            complement(59158..>60081)
                     /locus_tag="ACHLAM_000252"
     CDS             complement(59158..>60081)
                     /locus_tag="ACHLAM_000252"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFO7851844.1"
                     /translation="NSISICFTGFDQSDKSRLSALAKENGIDVKSDVGKSLFFLCVGK
                     NKGWRKVQKASDYGVIILTEEQFINFIDTGEIPLEQDCVVENDISKSLEKDVNTYKSM
                     IDEFSLSIRTVREFRRSNALIAVFVDGYAAGWRFAVKECYKDSLDIKLTPVKTEFFNY
                     EIWTQGDSFQFNRGDVFYSDRLAYDSYCDFLKLDDAVVLQVKYECFSGYDSVSTFDGV
                     MDGEFSFSRMEPRPVHKLPVLIETQLYDPGKIIVDVFVPDAAKKQLKLKDTLELKQDE
                     FVCLLQSGCCWVKRREAGDKKKPEFINLFKK"
BASE COUNT        14856 a        16671 c        15766 g        12788 t
ORIGIN      
        1 atatcaaaaa acgctctttt cacaaactcg ccgtaaacct ggagttattt acggcgaaaa
       61 gtcatcacgc catcagcatc ttcactactg ctttgcgaac ccgggctggc gcgcccactg
      121 cgcacagcgg tttatgcact tcccccggat aaaacacaac aaaatcacct tcattaagga
      181 taactgtttt ctcatcaacg ccttccggca aaaatgcgat gtctttatca gctaaccagt
      241 cggtatccgg cgtacctgca ggttgtgtgc tgaaggtcat gccttcctga ccttttaaca
      301 caatctgaat gtcgagatag cgggcatggt actccgcacg gcgagcttcg tacggctcgg
      361 tcatatcttc cgagataaga taaaacagac gattgccttc gatatcgtgc ttgcccttgg
      421 gcgtttctgc cgtaacgttt gctttgatat gctcaatcgc ctggcgtaac tcttggggta
      481 gccacggctg aagattatga atatttccga tgatcatctt ttccactcct taattaaaac
      541 aacgtttcat ttctatcgtt atacgaaaag atgatcaatt ccgctagcct taacgcgtga
      601 attattgtct tatcacaact tatcggcagg cgcgttcccg cctttcgggt caaggcccgc
      661 atcactcctc gagaatagtt tttatcgctt cccccgccag ggcagctgtt gcacagagtt
      721 gttcgatgga gtgcccctcc cgtgccttcg cagaaagccc tgacatcacg gtactcataa
      781 aatcagtcac acattgtgcg ctttgtggat gccgcctggc gatatagtca taaatggtcg
      841 tttctgcggc gtgataatat tgaatggcaa tatcacgcgc ttgtggatca tgactatgaa
      901 taccttcaag aaccatacag ccagcgcagc cgccgttttg gctatatctg cgcgccgctt
      961 cttttaatac ctcaaccagg cactcgccta ccggacgatc atcacgaaga atatcggcaa
     1021 gcggaatagc ttccgtaccg acatattcat tgagtacacg gctaaacaac ccagccttac
     1081 tgccaaaagc cgcgtagagg ctcggggggt taataccaag ataatcagta acttcagaga
     1141 cacttactgc atcgaaacct ttttggtgaa aaagcacttt tgccgcagag actgcctgct
     1201 caggagcgaa acgtctgggg cgacctggaa cgcgagattg ttttttagtg accattacaa
     1261 aacttgttga cagaaagtta aaacagtttt gtaatgcatg ttacataata aatcaaggag
     1321 tccttatggg cgcttttacc ggtaagacag ttctcatcct cggtggtagc cgtggaatcg
     1381 gtgccgctat cgtacgtcgt ttcgtcaccg atggagccaa tgtacgattc acctatgcgg
     1441 ggtcgaaaga tgccgctgaa cgtctggcac aagagactgg agcgacagca gtattcacag
     1501 atagtgctga cagagacgct gttattgatg tcgttcgtaa gagcggcgca ttggatatcc
     1561 tggtggtaaa tgcaggtatt ggcgtctttg gcgatgccct ggaattaaat gccgacgata
     1621 ttgatcgcct tttcaaaatc aatattcatg ctccttatca tgcctctgtt gaagccgccc
     1681 ggcagatgcc cgaaggcggg cgcatcttaa tcatcggctc cgtgaatggc gatcgtatgc
     1741 ctgttgcagg catggctgct tatgccgcca gtaaatctgc cctgcaaggc atggcgcgcg
     1801 ggctggcccg tgattttgga ccgcgaggga tcaccatcaa cgtcgtccag ccaggaccaa
     1861 ttgataccga cgctaatccc gccaacgggc caatgcgcga tatgttgcat agttttatgg
     1921 ccatcaaaag acatgggcaa ccggaagagg tcgctggtat ggtcgcatgg ttagcagggc
     1981 cagaagccag ttttgttacc ggcgcgatgc ataccattga tggcgcgttt ggcgcataac
     2041 cgactacgct caattaagcc cagccattat ccatgatgtc tgggttttgt ttactaacgt
     2101 cgtcccccta aaagcggctc ccggtaaata taaatcttct gttcatgtcg taaatcttga
     2161 ggataaccaa atggtagaaa gaaccgctgt tttccctgct ggccgacatt cactgtatgc
     2221 tgagcatcgt tattctgcgg ctattcgttc cggcgatttg ctgtttgttt ccgggcaagt
     2281 aggaagtcga gaggacggaa caccagaacc cgattttcag caacaagtca gactggcatt
     2341 tgataatttg catgcgaccc tggctgctgc gggatgcact tttgacgata tcattgatgt
     2401 tacgagcttt cataccgatc cagaaaaaca atttgaagac atcatgacgg tcaaaaatga
     2461 aatatttagc gccccacctt atccaaactg gacggcggtg ggtgttacct ggctggcagg
     2521 ctttgatttt gaaattaaag tgatagcgcg catccctgag ctataagcaa tagtgttagc
     2581 cgttcgtttt cacactccgc cctataagtc ggatgaatgg aataaaatgc atatctgatt
     2641 gcgtgaaagt gaaaaagaaa aaagcaggaa atgtctgcaa ttattgatac cgaacgacag
     2701 ttctccccca gaatcacatc aaataaaaat gcatgtattt tgacttttaa ttcaaataaa
     2761 ccgtttgcgc tgacaaaata ttgcatcaaa tgcttgcacc gcttctgacg atgagtataa
     2821 tgccggacaa tttgccggga ggatgtatgg ttcagtgtgt tcgacatttt gtcttaccgc
     2881 gtctgaaaaa agacgctggc ctgccgtttt tcttcccgtt gatcacccat tcccagcccc
     2941 tcaatcgagg ggcttttttt tgcccaggcg tcaggagata aaagatggct aatccgctat
     3001 atcagaaaca tatcatttcc ataaacgacc ttagtcgcga tgaccttaat ctggtgctgg
     3061 cgacagcggc gaaactgaaa gcaaacccgc aaccagagct gttgaagcac aaagtcattg
     3121 ccagctgttt cttcgaagcc tctacccgta cccgcctctc tttcgaaact tccatgcacc
     3181 gcctgggtgc cagcgtggtg ggcttctccg acagcgccaa tacatcactg ggtaaaaaag
     3241 gcgaaacgct ggccgatacc atttcggtta tcagcactta cgtcgatgcg atagtgatgc
     3301 gtcatccgca ggaaggtgcg gcgcgcctgg ccaccgaatt ttctggcaat gtaccggtac
     3361 tgaatgccgg tgatggctcc aaccaacatc cgacgcaaac cttgctggac ttattcacca
     3421 ttcaggaaac ccaggggcgt ctggacaatc tccacgtcgc aatggttggt gacctgaaat
     3481 atggccgcac cgttcactcc ctgacccagg cgctagcgaa gttcgacggc aaccgttttt
     3541 actttatcgc gccggatgcg ctggcaatgc cgcaatacat ccttgatatg ctcgatgaaa
     3601 aagggatcgc ctggagtctg cacagctcta ttgaagaagt gatggcggaa gtagacatcc
     3661 tgtacatgac ccgcgtgcaa aaagagcgtc tggacccgtc cgagtacgcc aacgtgaaag
     3721 cgcagtttgt tcttcgcgcc agcgatctcc acaacgccaa agccaatatg aaagtgctgc
     3781 atccgctgcc gcgtgttgat gagattgcga cggatgtgga taaaacgcct cacgcctggt
     3841 acttccagca agcaggcaac gggattttcg ctcgccaggc gttactggca ctggttctga
     3901 atcgcgatct ggtactgtaa ggggaaatag agatgacaca cgataataaa ttgcaggttg
     3961 aagctattaa acgcggcacg gtaattgacc atatccccgc ccagatcggt tttaagctgt
     4021 tgagtctgtt caagctgacc gaaacggatc agcggatcac cattggtctg aacctgcctt
     4081 ctggcgagat gggccgcaaa gatctgatca aaatcgaaaa tacctttttg agtgaagatc
     4141 aggtagatca actggcattg tatgcaccgc aagccacggt taaccgtatc gacaactatg
     4201 aagtggttgg caaatcgcgc cccagtctgc cggagcgcat cgacaatgtg ctggtctgcc
     4261 cgaacagcaa ctgtatcagc catgccgaac cagtttcttc cagctttgcc gtgcgaaaac
     4321 gcgccaatga catcgcgctc aaatgcaaat actgtgaaaa agagttttcc cataatgtgg
     4381 tgctggccaa ttaattgcgg ttggtaataa aagtctggct ccctataatg agccagactt
     4441 tttaccgctg taataaagga gaaatcatga gcaaaactat cgcgacggaa aatgcaccgg
     4501 cagctatcgg tccttacgta cagggcgttg atctgggcaa tatgatcatc acctccggtc
     4561 agatcccggt aaatccgaaa acgggcgaag taccggcaga cgtcgctgca caggcacgtc
     4621 agtcgctgga taacgtaaaa gcgatcgtcg aagccgctgg tctgaaagtg ggcgacatcg
     4681 ttaaaaccac cgtgttcgtg aaagatctga acgacttcgc aaccgtaaac gccacttacg
     4741 aagcgttctt caccgagcac aatgccacct tcccggcacg ttcttgcgtt gaagttgccc
     4801 gtctgccgaa agacgtgaag attgagatcg aagcaatcgc tgttcgtcgc taatcttgat
     4861 ggaaatccgg gctatcatgc ccggattaag tctgatgaca aaagcaaaat cgcccgatgc
     4921 gctacgctta tcaggcctac gtgattcctg caatttattg aatttgtagg ccggattggg
     4981 cgtttacgcc gcatccggca tgaacaaagc tcactttgtc tgcaatctga atccgggcca
     5041 ccatgcccag tttattcttt actgccagcc gtaacgacgg ctatagaccc ctttcaccaa
     5101 ctgggttaac gtcatatacc ctgccagaat cgcaaccagc cacgggaaat agcttaacgg
     5161 cagcgcctgc agctgcagat aactggccag cggtgaaaac ggcaatgcga tcccgacaat
     5221 catcacgatc acggtcatga tcattaacgg ccacgatgca cagctctgaa taaacggtac
     5281 acgacgggta cggatcatat gcacaatcag cgtttgcgac agtaagccca ccacaaacca
     5341 gcccgactgg aacagcgttt gcgtttccgg cgtgttggca tggaataccc accacatcag
     5401 gcaaaacgtc agaatatcga agatcgagct gatcggtccg aagaagatca taaagcgccc
     5461 cagatccgcc ggattccaac gctgcggctt ttgaatttgc tcgtcgtcga cgttatcaaa
     5521 cgggatcgcc acctgtgaca catcgtacag caagttctga ataagcaagt gtaacggcaa
     5581 catcggcagg aagggcaaga aagcactcgc caccagcacg ctgaacacat taccgaagtt
     5641 agaactcgcc gtcattttga tgtatttcag catgttggcg aaagtgcgac gtccctcaat
     5701 aaccccctct tccagcacca tcaggctttt ttccagcagg atgatatcag ccgcttcacg
     5761 ggcaatatct accgcgccgt ccacagaaat gccgatatcc gcagcgcgta aggcgggcgc
     5821 atcattaata ccatcgccca taaagccaac cacatgccct tcgcgcttca gtaaggtcac
     5881 aatgcgttct ttatgcatcg gcgtcaggcg ggcaaacagc gtggtgcgct gtgcgagatt
     5941 tgccagttca tcgtcagata gcgtttcaat atcactacca atgaccacct ctcccgcatc
     6001 cagccccact tcatggcaca ctttcgctgc aactaactca ctgtcgccag taaggatttt
     6061 tacggtaatt ccactcgctt ttaatgcctt cagtgccgga gcggttgtct cttttggcgg
     6121 atcaaggaaa gcaatatatc cttcgaggat caggtcggat tcatccgccc gctggtaatc
     6181 tccttcacgc gctggcaggt atttcgtcgc caccgcaacc acgcgcagcc cctgacgatt
     6241 cagcgtatca gtaacccgct taatcttacg cagcatgatg tcatcgagcg gcacaatctc
     6301 gccattgtga cgcacctgcg aacacacatt gaggatttcc tgcaatgcac ctttgcaaac
     6361 cagctgatgg tgttcggtat tttctgccac taccaccgac atccgacgac gctcgaaatc
     6421 aaacggaatc tcatcaattt tttgccaacg gctggccagc gaacgcgctg actcttcatc
     6481 cgtaccttcg agcaccgcgg tatcaagcag gtttttaagt ccggtctggt aatgactgtt
     6541 caaccacgcg ctatgcagca cgcgttcgct ggttttaccg gagatatcgg tatgattctc
     6601 cagcacaatt ttgtcctgcg tcagggtgcc ggttttatca gtgcacagaa tatccattgc
     6661 gccaaagttc tgaatggcat ccagatgttt gacgatcact ttctgtttcg acagctttac
     6721 tgcgccgcgc gccagcgtcg aggtaacaat catcggcaac atttctggcg ttaagcctac
     6781 cgcgaccgaa agcgcaaaca gtgccgcttc ccaccagtcg cctttggtgt aaccgttgat
     6841 aagcaacacc accggagcca tcaccagcat aaagcgaatc agcagcatac tgacgcggct
     6901 aatcccttgc tgaaaggcat tcggctcgct ttcctgctca ctaacacgcc ccgccagttg
     6961 accaaaccag gtattggccc ctgtggcaat caccattgct tgtgccgtac cgctcaccac
     7021 ggtggtgccc ataaaacaca gggtgtcgca ctccagcgga ttgctgtgct ccggctggcg
     7081 agtagtagcg gctttttcca cgggcagaga ctcaccggtt aacgacgcct gagcgacgaa
     7141 cagatcccgc gcctgcaaga tacgtaaatc tgccgggatc atatcccctg ccgccagttt
     7201 tataatatcg ccgggcacca gttggtcgat cgggatctcc agccagccat tttcgccttt
     7261 gtcgttaatt acgcgcagca ccgtcgcagt attgctgacc atcgctttca gggcatctgc
     7321 cgctttagtg gaacgtgctt cctgaataaa gttcagcaac gtagaaatag cgaccattag
     7381 cgcgataacg cccgcggcaa ataaatcttc cgtggcgtaa gaaatagcgc cgagaatggt
     7441 gagtaaaata ttaaaggggt tgcgatagca gacccataaa tgaacccacc acggcgacgg
     7501 ttgttgtgcg ggtaatttat tttcaccatg ttgttcgcgg gcagattcca cttccgcctg
     7561 atttaatcct tccggatggg tgtcgaacgt tttccacaat tcttcttcgg gcatcaccgc
     7621 cattttcagg caatgcgcac ttaaggacgg cgggaccgcc gtattcactg tctgctgcgc
     7681 gccaggcaat ggatcgcgat gaaccagacg ggaaggtaaa tggcgaatga accgggtaaa
     7741 aatttcttta aacataagga gtccctccgc actgtctgaa acagtacaga aatgtcttca
     7801 ggcgtggcga agccttacct gaaggtaata aagctgagca gggacgttaa aacgtcacgg
     7861 ccttacgcat ccggtaacgc cgtgaaaaga cgggcttacc ggaaaagttt taatctccgt
     7921 cgagggagag gcgtgggatc aggttccata taacctccgg taagtgaaaa aagcgccgca
     7981 aataataagg agaatccaat aatattcgta gcatttatgg cgatataata cctgctggca
     8041 actaaaccaa cgataaacca gactttaccc attgctgaat gcacaggtaa agttaggctc
     8101 aaataattaa acaacacgtt atgggacaac aggatgcaaa atcggctgac catcaaagac
     8161 atcgcgcgct taagcggcgt ggggaaatct acggtttccc gggtgctgaa taacgaaagc
     8221 ggcgtgagcc agcgcacgcg cgagcgtgtt gaagcagtga tgaatcagca tggattttcc
     8281 ccttcccgct ctgcgcgcgc tatgcgtggg caaagcgata aagtggtcgc catcattgtt
     8341 acccgtctgg attcgttgtc agaaaatctc gccgttcaaa ccatgctgcc agcgttctat
     8401 gaacaaggtt acgatccaat catgatggaa agtcagtttt ccccacaatt agttgccgaa
     8461 catttggggg tgctgaaacg gcgtaatatc gacggcgtag tgctgttcgg ttttaccggc
     8521 ataacagaag aaatgttagc ccactggcag tcatcgctgg ttctgctggc gcgtgacgca
     8581 aaaggctttg cttcggtctg ttatgacgac gaaggggcaa tcaagatcct gatgcaacgg
     8641 ctgtatgacc agggacatcg taatatcagt tatctcggcg tgccgcacag tgacgtgacg
     8701 accggtaagc gacgtcacga agcctacctg gcgttctgca aagcgcataa attgcatccc
     8761 gttgccgctc tgccagggct tgctatgaag caaggctatg agaacgtagc aaaagtgatt
     8821 acgcctgaaa ctaccgcgtt actgtgcgca accgacacgc tggcacttgg cgcaagtaaa
     8881 tacctgcaag agcaacgcat cgacaccttg caactggcga gcgtcggtaa tacgccgtta
     8941 atgaaattcc tccatccgga gatcgtcacc gtagatcccg gttacgccga agctggacgc
     9001 caggcggcct gccagttgat cgcgcaggta accgggcgca gcgaaccgca acaaatcatc
     9061 attcccgcca ccctgtcctg atcgtttcct gaacgataaa ttgtgatctt cgctgcgttt
     9121 cgggaacgtt cccgttttta aatttttcag cgcaatatat tctgcagcca accaaaaatg
     9181 tcatctgcca tggggcttta tgatgagcaa aataaaccaa acggatatcg atcggttgat
     9241 tgaactggtc ggcgggcgcg gcaatattgc gacggtgagc cactgtatta ctcgtctgcg
     9301 ctttgtcctc aaccaaccgg ccaatgccag accgaaagaa attgagcaac tccccatggt
     9361 gaaaggctgt ttcaccaatg ccgggcaatt tcaggtggtg attggcacca acgtgggtga
     9421 ttactatcaa gcactgatag cgtcaaccgg acaggcgcag gttgataaag agcaggtaaa
     9481 aaaagccgcc cggcagaata tgaaatggca tgagcagttg atctctcatt tcgcggagat
     9541 tttcttcccg ttgctgcccg cgttgattag cggcggtttg atcctcggtt ttcgcaatgt
     9601 gatcggcgat ttgcccatga gcaacggtca aacgctggcg caaatgtacc cttccctgca
     9661 aacgatctac gattttctgt ggttgatcgg tgaagcgatc ttcttctacc tgccggtcgg
     9721 gatttgctgg tcagcggtga aaaaaatggg cggcacgccg atccttggta tcgtgcttgg
     9781 cgtaacgctg gtttctccac agctgatgaa cgcttatctg ctcgggcagc agctgccgga
     9841 agtgtgggac tttggcatgt tcagcatcgc caaagtgggc tatcaggcgc aggtgatccc
     9901 ggcactgtta gccgggctgg cgctgggcgt tattgaaact cgtcttaagc gcatcgtgcc
     9961 ggattacctc tatctggtgg tggtgcccgt ttgttcgctg atcctcgcgg tgttcctcgc
    10021 ccatgcgctg attggtccgt ttggtcgcat gattggcgat ggcgttgcct ttgcggtacg
    10081 tcacctgatg accggcagct ttgctccgat tggcgcggca ttgtttggct tcctgtacgc
    10141 cccgctggtg atcaccggcg tacatcagac cacgcttgct attgatttgc agatgattca
    10201 aagcatgggt ggtacgccag tgtggccgct gattgcgctg tcgaatatcg ctcagggttc
    10261 cgccgtgata ggcattatca tttccagccg caagcacaat gaacgcgaga tctccgtgcc
    10321 tgccgctatc tccgcctggc ttggagtcac tgagcctgct atgtacggca tcaacctgaa
    10381 atatcgcttc ccgatgctgt gcgcgatgat tggttctggt ctggcaggat tgctatgcgg
    10441 cctgaacggc gttatggcga atggcatcgg cgtaggcggc ctgccgggaa ttctctcgat
    10501 tcaaccgagc tactggcagg tgtttgcgct ggcaatggct atcgccatca tcatcccgat
    10561 tgtactcacc tcgtttatct atcagcggaa ataccgcctg ggcacgctgg acattgttta
    10621 attttcttcg gggcgcaatt gcgccccctc gcatttgcag gaataacgta atgactaatc
    10681 ttccccactg gtggcaaaac ggcgttatct accagattta tccaaagagt tttcaggaca
    10741 ccacgggtag cggcaccggc gatttacgtg gcgttatcca gcgcctggac tatctgcata
    10801 aactgggcgt tgatgccatc tggctaaccc ccttttatgt ctctccccag gttgataacg
    10861 gttacgacgt agcgaactat acggctattg atcccaccta cggcacgctg gacgattttg
    10921 acgaactggt gacacaggca aaatcgcgcg ggattcgtat cattctcgat atggtgttta
    10981 accatacctc tacccaacat gcctggtttc gcgaggcgct gaacaaagca agcccttacc
    11041 gccagtttta tatctggcgc gatggagaat cagaaacgcc accgaacaac tggcgttcaa
    11101 aatttggcgg tagtgcgtgg cgctggcatg cggaaagcga acagtactat ttgcatctct
    11161 ttgcaccaga acaggcggat ctcaactggg agaatccagc ggtacgcgca gagctgaaaa
    11221 aagtctgtga gttctgggcc gatcgtgggg tcgacgggtt gcgcctggat gtggtgaatc
    11281 tgatctccaa agacccgcgt ttccctgatg acctggatgg cgacgggcgt cgcttctaca
    11341 ccgacgggcc acgagcacac gagtttttgc acgagatgaa ccgcgatgtg tttacgccac
    11401 gcaggttaat gaccgtaggt gaaatgtcct ccaccagcct tgagcattgc cagcgatatg
    11461 cgacactgac aggcagtgaa ttgtcgatga cctttaattt tcatcacctg aaagtcgatt
    11521 atcccagtgg tgaaaaatgg acgctggcta aacctgactt tgtggcgttg aaaacattgt
    11581 tccgccactg gcaacaagga atgcacaacg tggcatggaa tgccttgttc tggtgtaacc
    11641 acgatcagcc gcgcattgtt tctcgctttg gtgatgaagg tgaataccgc gtgcctgcgg
    11701 caaaaatgct ggcgatggtg ctgcatggca tgcagggaac gccgtatatc taccagggcg
    11761 aagagattgg catgaccaac ccgcatttca cgcgcattac tgactatcgc gacgtggaga
    11821 gcctcaatat gtttgccgag ctgcgcaacg atggtcgtga tgccgacgag ttattggcaa
    11881 tcctcgccag taaatcccgt gacaacagtc gcacacccat gcaatggagc aacggcgata
    11941 atgccgggtt tacggctggc gaaccgtgga ttggcctggg cgataactat caggaaatca
    12001 acgtagaagc agcgctggcc gatgagtcct cggtgtttta cacctaccaa aagttaatcg
    12061 cactgcgtaa gcaagaagcc gtcctgacat ggggcgatta ccaggatctg ctgccaaaca
    12121 gccctgtatt gtggtgctat cgccgtgagt ggaaggggca aaccttactg gtcattgcca
    12181 accttagccg tgagacccaa ccctggcagc cagggaaaat gctcggcaac tggcagcttg
    12241 tgatgcataa ctacgaagaa gcctcaccac aaccctgtgc catgacttta cggccttttg
    12301 aggctgtctg gtggttacag aagtaagtat ccgctaagcc ccggtaatac tggtcattcc
    12361 ggggttttgc ctttcagcat gttgatttta taatcaaaaa attcattaaa aaatttacat
    12421 aacgctgtag cgcccgtcat ccgtacgctc tgctttttac tttgagctac atcaaaaaaa
    12481 gttcaaacat ccttgatgca aagcactata tatagacttt aaaatgcgtc ccgacccaat
    12541 atgttgtatt aatcgactat aattgctact acaactcccc acgaaaaagg tgcggcgttg
    12601 tggataagcg gatggcgatt gcggaaagcg ccggaaaacg aaacgaaaaa accggaaaac
    12661 acctttccca atttctgtgg ataacctgtt cttaaaaata tggagcgatc atgacaccgc
    12721 atgtgatgaa acgagacggc tgcaaagtgc cgtttaaatc agagcgcatc aaagaagcga
    12781 ttctgcgtgc agctaaagca gcggaagtcg atgatgcaga ttattgcgcc actgttgccg
    12841 cggttgtcag cgagcagatg cagggccgca gccaggtgga tatcaacgag atccagaccg
    12901 ccgttgaaaa ccagcttatg tcgggtccat acaaacaact ggctcgtgct tacatcgagt
    12961 accgtcacga tcgcgacatt gaacgtgaaa aacgcggtcg cctgaatcag gagatccgtg
    13021 gtctggttga gcagaccaac gcctcgttgc tcaacgaaaa cgccaacaaa gacagcaagg
    13081 tgattccaac ccagcgcgac ctgctggccg ggattgtggc taaacactat gcacgccagc
    13141 acctgctgcc gcgtgacgtg gtgcaggcac atgaacgtgg tgatattcac tatcacgatc
    13201 tcgattactc accgttcttc ccgatgttca actgcatgtt gatcgacctg aaaggcatgc
    13261 tgacccaggg ctttaaaatg gggaacgccg agattgaacc gccgaagtcg atctctacgg
    13321 caaccgcggt aactgcgcag attattgccc aggtcgccag ccatatttat ggcggcacca
    13381 ccattaaccg tatcgatgaa gtgctggcac cgtttgtcac cgccagctac aacaaacatc
    13441 gcaagaccgc agaagagtgg aacatcccgg acgccgaagg ctacgctaac tctcgtacca
    13501 tcaaagagtg ctacgatgcc ttccagtcgc tggagtacga agtaaacacc ctgcacaccg
    13561 ccaacggtca aacgccgttt gtaacctttg gttttggtct gggcaccagc tgggagtcgc
    13621 gcctgattca ggaatctatc ctgcgtaacc gtatcgccgg cctcggtaaa aaccgtaaaa
    13681 cggcggtgtt cccgaaactg gtgtttgcga ttcgcgatgg cctgaaccat aaaaaaggcg
    13741 atccgaacta cgacatcaaa cagctggcgc tggagtgcgc aagcaagcgc atgtatccgg
    13801 atatcctgaa ctacgatcag gtagtgaaag tcaccggttc gtttaaaacc ccgatgggct
    13861 gccgcagctt cctcggcgtg tgggaaaatg aaaacggcga gcagatccac gatggtcgta
    13921 acaacctcgg cgtgatcagc ctgaacctgc cgcgtattgc tctggaggca aaaggcgatg
    13981 aagccacctt ctggaagctg ctggatgaac gtctggtgct ggcacgtaag gcgttgatga
    14041 cccgtatcgc tcgtctcgaa ggcgtgaaag cgcgcgtggc cccgatcctc tatatggaag
    14101 gtgcttgtgg cgtgcgtctg aatgctgacg acgacgtttc tgaaatcttc aaaaacggtc
    14161 gtgcgtctat ttcgctgggt tacatcggca tccacgaaac cattaacgcg ctgttcggcg
    14221 gcgagcatgt ttacgacaac gagcagctgc gcgcgaaagg tattgcgatt gttgagcgtc
    14281 tgcgtcaggc ggtggatcag tggaaagaag aaacgggtta tggtttcagt ctctacagca
    14341 cgccaagtga aaacctgtgc gaccgcttct gccgtctcga taccgctgag tttggcgtgg
    14401 tgccgggcgt gaccgataaa ggttactaca ccaacagttt ccaccttgat gtggagaaaa
    14461 aggtgaaccc gtatgacaag atcgactttg aagcgcctta cccgccgctg gcgaacggtg
    14521 gtttcatttg ctacggcgag tatccaaaca ttcagcacaa cctgaaggcg ctggaagatg
    14581 tctgggatta cagctatcag catgtaccgt attacggcac caatacgccg atcgatgagt
    14641 gctacgagtg tggctttacc ggtgagttcg agtgcaccag caaaggcttc acttgtccga
    14701 aatgtggtaa ccatgacgcc tcccgtgtgt cggtgactcg ccgcgtgtgc ggatatttag
    14761 gtagcccgga tgcacgtcca tttaacgccg gtaagcagga agaagttaag cgccgcgtta
    14821 aacatttggg gaatgggcag ataggttaat cttcactctg cgttaagcaa atgccggatg
    14881 cggcgtaaac gccttatccg gcctacattt ggcaacggct gtaggcctga taagacgcga
    14941 cagcgtcgca tcaggcgttg attactggat gcagcctaca cgcggactga taagccaggc
    15001 gaaataatga attatcatca gtactatcct gtcgacatcg tcaacggccc cggcactcgc
    15061 tgcaccctgt ttgtctccgg gtgtgttcat gaatgccccg gttgctataa caaaagcacc
    15121 tggcgggtaa attccggtca gccatttacc aaagcaatgg aagaccagat cattaacgat
    15181 ctgaatgaca ctcgcatcaa acgccagggt atttccctct ccggcggcga tccgctgcat
    15241 ccgcaaaacg tgccggatat tctgaaactg gtacaacgca tccgcgccga atgtccgggt
    15301 aaagacatct gggtgtggac aggctataaa ctcgacgaac tcaacgctgc gcaaatgcag
    15361 gttgttgatt tgattaacgt gctggtcgac ggcaaatttg tgcaggattt aaaagaccct
    15421 tccctgatct ggcgcggcag cagcaatcag gtggtgcatc atttgcgatg ataagttccc
    15481 tgttacctga gtgcaggcaa cagggaaata aggaattaac gatacttctg gtgataggcg
    15541 ttgcgggtcg ttttcagttg ctctgcggca gcctgcgctt cttttacttt accttcattt
    15601 gccagcttca gcgcgtcgtc aatctgaccg accagaatat cgaaaccgtg gcggaagtct
    15661 ttcatttccg ggctgtccgg tgatttgcct tcgagcttcg gcggcgtcgc tttttgcgca
    15721 tccagcgctg cggcgcgcat cttcgttaac gcgtctttga cttgcgccgc gttatcggct
    15781 ttttcgacca cttttaaatt gtcgttgagg gtttccatat tgtcttcaag atcagcggca
    15841 aacgacgccg aactgaatac caacgaggag actgcaagaa tagctaacag gcttttacgc
    15901 attgctcact tcctttttta tttacacttc atccttcaca ctgcctcatc gacggctacg
    15961 ctcattcgcc cgagtcacgt actgcgagta cgctccccgg tcctcattca ctttccgtct
    16021 cgatgcagtg cgaatgattt tgtgtaattt tttagtttat agcgcggcag gtcgcgccag
    16081 ttttttactg cccggcgatt ttcatctccg gcaacagcac agaaccacac tgtatattac
    16141 tgcgtgtttc aatatcgtta ccgacggtga caatattgcg ccacatatct tttaaattac
    16201 ctgcgatggt gatttcgcta accggatact gaatttcgcc attctctacc cagaagcccg
    16261 ctgcaccacg ggaataatca ccggtaatgg cactcacgcc ctggcccatc agctccgtca
    16321 ccaccagccc ggtgcccatc tctttgagca tttgttcgaa gcttagccct tgtccggcaa
    16381 tccgccagtt gtgaataccg cccgcatgtc cggtgctttt cagccccagt ttccgcgccg
    16441 aatagctggt cagcagccac tgagtcagga tgccatcttt aataatatcg cgacgctcgg
    16501 tgcgcacacc ttcgctgtcg aatggcgtag aggccagccc tttcagcaga tgcggatgct
    16561 cttcaatggt cagccagtcc ggcagaattt gtttacccag cgaatccagc aggaaggtag
    16621 atttacggta aaccgatcca cccgctatcg ccccaaccag atgcccaaaa agcccggttg
    16681 ccacttcatt ggcaaaaatg accggcgctt tcatggtgga gagtttacgc ggtgacagac
    16741 gcgataaagt gcggcgagca cagtcggccc cgacccactc tggcgtttgc agatcgctca
    16801 tcgcacgacc aatggtgtag gcgtaatcac gctccatatc gccattttct tcggcaatta
    16861 cacagctgga gagcgaatga cgcgtagagc agtaaccctg caacatgccg tggctgttgc
    16921 caaaaacttt gacaccgtag tggctgttaa agctgccacc ttcggtattg gtgatgcgtt
    16981 tgtccgcctg caatgctgcc tgttctgcgc gggccgccag ttcaatggct tcatccgggg
    17041 aaacttccgc agggtggaac aagtcgagat ccggtgcgtc aaaggccagc agctctttgt
    17101 ctgccacgcc ggcacaggga tctggcgagg tataacgggc aatatctagc gccgcctgta
    17161 cggtgcgggc aatggcctgc gggcttaaat cggtagacga tgcgctacct ttgcggttct
    17221 ggtgataaac agtgatcccc aacgcgccat cgctattgaa ttcaacattc tccacttcac
    17281 cataacgcgt gcttacgcta atgccggtgg tcttgctgac ggcaacttct gcaccgtccg
    17341 atttgcctga ggccaactcc agcgcagtcg aaactgcttc ttccagaatc ttgcgctgcg
    17401 cttcaacttg agagattact ttcattgcaa gtgccataat gtagagagtt tctctgaagt
    17461 ctaacagaga accgtttttc agtgcgcatc ttaactggta acattagcct ctttttttaa
    17521 ggagcctgag atgactaagc agcccgaaga ctggctcgac gacgttcccg gtgatgacat
    17581 cgaagacgaa gacgatgaaa ttatctgggt cagtaaaagt gaaattaaac gcgatgccga
    17641 ggagctaaaa cgccttggcg cggaaattgt tgatctgggg aaaaacgcgc tggataagat
    17701 cccgttagat gcggatctgc gcgcggctat tgagctcgcc cagcgtatta agatggaagg
    17761 tcgccgccgc cagttgcagc tcatcggtaa gatgctgcgc cagcgcgatg ttgagcctat
    17821 tcgtcaggcg ctggataagc tgaaaaaccg ccacaaccag caagtggtgt tgttccataa
    17881 actggaaaac ctgcgcgatc gtctgatcga gcaaggtgat gacgccatcg ctgaagtatt
    17941 gaatctgtgg cccgatgccg atcgtcagca actgcgtact ctgatccgta acgcgaagaa
    18001 agagaaagaa gggaataagc cgccgaaatc cgcgcgccag attttccagt atctgcgtga
    18061 gttagcggaa aacgaagggt aattttcgtt gttgatgtga gtatggtttg ccggatgcgg
    18121 tgcacctgta ggcctgataa gacgcatcaa gcgtcgcatc aggcactatg ctcaaatgcc
    18181 ggatgcggcg tgaacgcctt atccggccta cacacactct atcaggctga ggctgtgaat
    18241 tactgcgcgg cttccgcttt cttcgctaac ccatccagca gtttctgatg gatcccacca
    18301 aaaccgccgt tactcatcac cagaatgtga tcgcccggct gagcggtttt caccaccata
    18361 tctgccagcg tatccacatc gccgctccag tgtgcaggct gaacgcaggc ttctgccact
    18421 tctgccacct gccacggaat atgcgccggt tgcagcagga agacttcatc ggcacgacct
    18481 aatgaaggtg ccagatcgtc tttgcagatc cccattttca tggtgtttga gcgcggttcc
    18541 agcacggcaa taatgcgcgc cgtaccacca actttgccac gcagcgccgc aagcgttgcc
    18601 agaatcgccg tcgggtgatg ggcaaaatcg tcatataccg tcacaccatt cgcttcacca
    18661 cgcaactcca gacggcgacg agcattaata aacgaaccca gcgcgttagc ggcatctgcc
    18721 ggcgctacac caacatggcg agccgccgca atcgccatca ggccattgtg catattatgt
    18781 tcgcctacca gcgaccattt cacttcgccc actttttcgc catccagcaa gacttcccat
    18841 tcggaagcat cggtggtcag ctttttcgcc tgccagtgac cttgctcgcc caccagctcc
    18901 tgctcgctcc agcagcccat cgccatggtc tgtttcaggt tgatgtcatt ttccggccag
    18961 ataatacggc cctgccccgg aacgatacgc accagatggt ggaactgttt ctggatcgct
    19021 ttcaggtcgt caaagatatc ggcgtgatcg aactcaaggt tgttgaggat cagcgtacgc
    19081 gggcagtaat ggacaaattt agagcgtttg tcgaagaagg cgcagtcata ctcatccgct
    19141 tcgataacaa agaagtcgct ttcgcccaga cgcgccgaaa cctcaaagtt ccccggcaca
    19201 ccgccgatca caaatcccgg tttgtaaccg cactgttcca gaatccaggt tgccattccc
    19261 gcggtggtgg ttttgccatg tgtaccggca acggccagca cccagcggtc gcgcagcaca
    19321 aaatcgtgca gccactgcgg acctgacata taagggatgt ttttttccag taccgcttcc
    19381 acacacggat ttccacgggt catggcgtta ccaataatca ccagatccgg ctgtggatcg
    19441 agctggctgg catcgtaacc ctgaatcagt tcaatgcctt gcttctcaag taaggtgctc
    19501 atcggcggat acacattggc gtccgaaccc gttacttcat ggcctaactg gcgcgccagc
    19561 atcgccagac cgcccataaa cgtgccacaa attcctaaaa tatgaatgcg catacgtcac
    19621 tatccttttt taatctggcg ctcattttac gcatatgtcg ggcaagtgag aaacgcattt
    19681 caggataatc cgtaatttgc tggcgcgatt cacctgagcg caacattggg attatttgtt
    19741 aagattgttg cggtcgcttt actccataaa cattgcaggg aaagttttat gaaaacgtta
    19801 ggtgaattta ttgtcgaaaa gcagcacgag ttttctcatg ctaccggtga gctcactgct
    19861 ttgctgtcgg caataaaact gggcgccaag attatccatc gcgatatcaa caaagcagga
    19921 ctggttgata tcctgggtgc cagcggtgct gagaacgtgc agggcgaggt tcagcagaaa
    19981 ctcgacctgt tcgctaatga gaaactgaaa gccgcactga aagcacgcga tatcgttgcg
    20041 ggcattgcct ctgaagaaga agatgagatt gtcgtctttg aaggctgtga acacgcaaaa
    20101 tacgtggtgc tgatggaccc actggatggc tcgtccaaca tcgatgttaa cgtctctgtc
    20161 ggtaccattt tctccatcta ccgccgcgtt acgcctgttg gcacgccggt aacggaagaa
    20221 gatttcctcc agcctggtaa caaacaggtt gcggcaggtt acgtggtata cggctcctct
    20281 actatgctgg tttacaccac cggatgcggt gttcacgcct ttacttatga tccttcgctg
    20341 ggtgttttct gcctgtgcca ggaacggatg cgcttcccgg agaaaggcaa aacctactcc
    20401 atcaacgaag gaaactacat taagtttccg aacggggtga agaagtacat taaattctgc
    20461 caggaagaag ataaatccac caaccgcccg tatacctcac gttatatcgg ttcactggtc
    20521 gcggatttcc accgtaacct gctgaaaggc ggtatttatc tctacccaag caccgccagc
    20581 cacccggacg gtaaactgcg tttactgtat gagtgcaacc cgatggcatt cctggcggaa
    20641 caagcaggcg gtaaagcgag cgatggcaaa gagcgtattc tggatatcat cccggaaacc
    20701 ctgcaccagc gccgttcatt ctttgtcggc aacgaccata tggttgaaga tgtcgaacgc
    20761 tttatccgtg agttcccgga cgcgtaatct cctgcgcctc tccacacgga gaggcgtttt
    20821 tatcgctgcg caatgttgac tcttgtcaca ggcgaactct gacgattctc ccacagcacc
    20881 gttaatccac gctgtaatgc tataaaaata aacaacaaaa taccgatggc gattttcgtc
    20941 caccatgagc tcagcgtgcc atcaaagttt atgtaagtct gaatcagtcc ctgaatcgcc
    21001 acgccaaaaa gcgtccctaa taccgttcca acgccaccac tcaaaagcgt accgccaatt
    21061 accactgagg cgatagcatc cagttccaca cctacgcccg ccagcgcata tccggcctgg
    21121 gtataaatcg agaagacaat ccccgccagt gtcgccagcc cggtagagag catataaatg
    21181 cgaatagtgg tgctgcgagt ggaaatcccc atcaggttcg ccgacgttgc gttgccgcca
    21241 atggcgtaca cctgattacc aaaacgcgta cgatgcgcga ggaatatgcc gataaccacc
    21301 accgccagca tcagcagccc catcgcactt aagcgaccgc cgccagggat tttccacgca
    21361 aggcttgaga gcgtgtcata aacaggatgg tttatcggga tcgactcttc cgaaacgaga
    21421 tagctgacgc cacgcaaaaa gaacattcct gccaaagtaa tgataaacgc cgggatcttc
    21481 agggcgtcaa tcagaagccc cataaatgcg ccgaaggcac agcccatcac caacaccagc
    21541 ggaaacgcca gcagcggcga gaggccgaaa tcgccaatca cttttgccag aaacacgccg
    21601 gtaaaggcga tcaccgaacc gacggagaga tcgatcccac cggagaggat cacaaaggtc
    21661 atgccaacgg cgattatccc aagaaaggca ttatcggtca ggatattgca gatcactctt
    21721 gtggaagcaa aaccgggaaa ctgggtcaga cagtagatat aacccaacac aaagacgccg
    21781 atggtgatca tcagtggcaa attacgtttt atcacggtta cgtacccctt taatcagact
    21841 gataaagcgt tgcgactgga caatcagcac gcaaagcacc accaccgctt tcaccacctg
    21901 gttcatctcc ggcggaaatc cagaaagcaa aattccggtg ttcattccct gaataatcag
    21961 cgcccccacc accgaaagca gcaggttaaa gcgcccgccc atcagcgatc cgccgccaat
    22021 caccaccgcg agaatggcgt ccagctccag ccataacccg gcgttattgg catcggcacc
    22081 gcgaatatcc gccgccacga taatgcccgc aatcgccgca catagcccgc tcaacacgta
    22141 ggtgagcatg acgatgattc gcgtgtttac cccggcattt tttgccgccc gaatattgat
    22201 accaacggct tcgataaaca tccccagtgc cgttttgcgg gtcaacagcc agaacaggag
    22261 aagcgtcagc accgcaataa tgactggcgt tggcaggaac aacagcgatc cactgccgaa
    22321 ccaggagaga tccggtgagt taaatgtgac gatctgtccg gaggtgatca gttgcgccac
    22381 accgcgcccg gcgaccatca ggatcagggt ggcgacaaac ggctgaattt tgaggatcgc
    22441 taccagtatg ccgttccaca atcctgccag gatgccagtg cccagggcgc ttaacaaaac
    22501 aatcggcagg ctgaatcccg cgaccgtcat cgcagccgtt gtggctccgg cgatagccat
    22561 caccgcccct acggagagat caatcccacc ggtggcgata accagcgtca tgccaatcgc
    22621 cagtagcgca acgggggccg cacggttaag aatgtcgata gggctaccga acaaacgccc
    22681 atcctgaagc accacctgcc agaaatgcgg ggccaccagg ctatcgacca gcagcaccag
    22741 caacagtgcc gccagctgcg gcattcctgt tggccagcga aaacgccttt tcggcggcgt
    22801 agtgtccggg agagattgag gcatcacact attctcctta cgccgcaatg gcgttcatga
    22861 tcgccggaac ggaaagctct gccagcggga tctccgccac ctgtttgcga tcgcgcatga
    22921 taatcacccg gtcggcatag cccaccagct cttccagttc agaggagatc accagcagcg
    22981 ccagaccgtc agcgcacagc gtttcaatca ggcggatgat ttcagcgtgg gcaccaacat
    23041 caatgccgcg ggttggctcg tcgaggatca aaaattgcgg acgagtcagt agccaacgtg
    23101 aaagcaacac tttttgctga ttgccgccag agagaaattc aatcggttgt tcagttgaag
    23161 gtgtgcgaat gccaagctgg cggataaagc gttcggcaat ctcttgctgt tctttgcggg
    23221 aaatcggtcg tagccagccg cgctgggcct ggagagcgag gatgatattt tcccgcaccg
    23281 aggcggcggc gatgatgcca tcggttttcc tgtcttccgg gcagaagccg atacccagta
    23341 ccgaagcctg atgtggcgat cgcagggttt gctgtttgcc tttgatcaac gccgtgccgc
    23401 tgtcagcagg tttgatcccg aagatcactt cggcggtttc ggtacgtccg gatcccagca
    23461 agcctgccag gccaacgatc tcgccggggc gtacttcgag atcaaacggt gcgatcgttc
    23521 cttttttgcc gtaatttttg aacgcggcaa cgggtttgtc gctcagcaat gttctcccgg
    23581 cacgctgaag cgcgtgggtg tccagttcac gccccagcat catttttacc agttcgatct
    23641 gcggtagctc gcgggtttcc cgacagccca caaaactgcc gttacgtaag acggtgatcc
    23701 gatcgctaac ctgataaacc tgatcgagaa aatgagtgac gaagatcagg ctgacgccgc
    23761 gatcgcgaag ctgacgcatc aggccaaaca gtaactctac ttcctgagtg tcgagactgg
    23821 cggtgggttc atcgaggatc agcactttgg cggagagatc gatagcccgg caaatggcga
    23881 cgatttgctg catcgcgact gaaaagcggt tgagcggttc gcgcacgtcg agggagaaac
    23941 cgtaagatgc catcagttcg gtggcgcgct tttccatctc tttacggcgt agaaggccga
    24001 aacgtttggg ttcgcgacct atgaatagat tatcagcgac cgacatattg ggtagcaggt
    24061 tgacttcctg atagacggtg ccgatgccga gttgttgtgc gtgggcggtg tttttcggtg
    24121 agatagcctg gccttccagc cagatagtgc cgcgatcggc gtggtataca ccggttaatg
    24181 ctttgattag cgtcgatttt cccgccccgt tttcaccgag cagcgccatg atttcgccac
    24241 ggcgcaggct gaaatcaacg ttgtctaacg ctttgacacc ggggaaaaat ttacttaatc
    24301 cttcggtgcg gaggatttcc tggtgttggt cggtcgtcat ggtttccccc ggtgtaaagc
    24361 gtgctcccag taggcatgat aagacgcggt aagcgtcgca tcaggcttca gcgcacaacc
    24421 gccggatgcg gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca tagcaaccct
    24481 caatacccca tatttttctt cttctctaac tcttcttttg cggtatcagg cagataaagg
    24541 gtggatttgg ttaacgtcag cttttcaggc atggtgccgt cttttttata tttctccagc
    24601 gcgtcgaagg cgggacctgc catattcggc gtcagttcaa cactggcgtt cgcttcgcca
    24661 tcaatcatcg ctttgtagat gtccggcaca ccgtcgatag agcctgtcag gatatctttg
    24721 cccggtttca ggcccgcttc tttaattgcc tgaattgcac caatcaccat gtcatcgtta
    24781 tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa gctctccatc
    24841 acttctttgc ctttgctgcg ggtgaagtca cctgactgcg agcggataat tttgatattt
    24901 ggcgcattct taatggcttc ggcaaagcct ttcttacggt caatggcgac gctggcccca
    24961 acggtgccct gcagctccac cacgttgcat ggtttgccat tcacttcttt taccagccag
    25021 tcaccaatca acttgccttc gaggatgttg tcggcggtga cagtggtcat atagagagat
    25081 ttgtctttca catcgatgga acggtcaagc aagaagaccg ggatttcggc atctttcgcc
    25141 tcttttaata ccggctccca accggtcgct accactggag cgataaagat cgcatccacc
    25201 ccttgcgcga cgaaggaacg taccgcttta atctggtttt cctgcttttg ctgaccatcg
    25261 gcaattttca acgtaattcc gcgcttttcg gcttcacttt tcgccacatt ggtttctgcg
    25321 gcgcgccagc cggattccga cccgacctgc gaaaatccaa cggttaatgg agcggccaac
    25381 gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt ccacataggg
    25441 ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcgaaaaaa ctatagacaa
    25501 ttcgttatgt aacggattgc gttacatcac acttcaaaaa agtaaataaa acgttaatca
    25561 caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat ggattttcct
    25621 gctctgtata ccgtcttaaa actggcgaaa aaggaaaatg aagacgaaaa caagcgaaga
    25681 cattcggcgc gagttggcta taatactcgg cacttgtttg ccacatattt ttaaaggaaa
    25741 cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac atctacgttg
    25801 ttattgagat cccggctaac gcagatccaa tcaaatacga aatcgacaaa gagagcggcg
    25861 cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac tacggttaca
    25921 tcaaccacac tctgtctctg gacggtgacc cggttgacgt actggtcccg actccgtacc
    25981 cgctgcagcc gggttctgtg atccgctgcc gtccggttgg cgtactgaaa atgaccgacg
    26041 aagccggtga agatgcgaaa ctgattgctg ttccgcacac caagctgagc aaagaatacg
    26101 atcacattaa agacgttaac gatctgccag agctgctgaa agcgcaaatc gctcacttct
    26161 tcgagcacta caaagacctc gaaaaaggca agtgggttaa agttgaaggt tgggaaaacg
    26221 cagaagccgc taaagctgaa atcgttgctt ccttcgagcg cgccaagaat aaataagttc
    26281 ttctggcgca ataaccctga acaccgggct tcggttagta agggtttttt tatgcccgca
    26341 atttataaac tctctactcc actattatta ttttcagcag ttgaaaggct tgaacggtaa
    26401 gaacaagcta aactcgacca ccattttgct gttcatagcc acttgctgga agttagccgg
    26461 cctcactcat actcaccgtt caaagcgtgt ccccgtttta cccctgtaca tttgttaacg
    26521 ccagcagagt tttttatgcg attgcagata tggaaagcgg cccggagaga aaagcaaaag
    26581 atgggaaagt tacaaaaaaa atcgcgagat acatcgcata aaacaacgcc acccgaaggt
    26641 ggcataaggg ggtagcatgt gccgcactac cccttgatat atcaaatagt tacaagttca
    26701 aagtggttcg aaatcgtgcc atttagtccc cgtttatggg caaaatctgg acaactccgc
    26761 aaccggattg aaccttaccg cgtcctggag ataatcaggt gcaaagtgcg cataggtcat
    26821 tgtttgctga attgtgctgt gccctaaaat acgctgaagg gtaataatgt tccctccatt
    26881 tatcatgaaa tgagttgcga atgtgtgccg taaaacatgc acagactgac cgactggtaa
    26941 atcaggccgc acagtcctta ttgcacttct gactatcgca taactggggt tcattaatct
    27001 tccagtcgct ttgactttaa tcagagattc aagatcttta gctataggaa taacccgccg
    27061 cgatccattc tttgtctcca tgaaaataac cttcccacca attatgtgtt cacctttaat
    27121 attggcaact tcaccccagc gcccgcctgt agccaggctt aacaatacag ccttaagatt
    27181 atcgccagat aaaagtgcgg ttagcctggg aatatcatca tgtgcaagat aagccatttc
    27241 cggctgatta attgttagct tgcgtatctc gtgaaacggg ttatcaccca caaactcttc
    27301 aacatcaatc agcttttcaa agaaaccgct catcattgca caataacgat ttaccgttga
    27361 ctgcttaatt ccgcgattca ttaattcaag gcgatatctg ataatatttt ttcgggttaa
    27421 gtggtcactt ctgttaatac caagctcttg catatcacga agaaccgctt caattctgac
    27481 ccgattcatc gcgccataac gatgattctt accgtggtac acccaccaca gttccagaag
    27541 atcgcttaac gctttacgtt caataggttt ttcgacccag tccttatcgt gataatgcgt
    27601 cagaacatgg cgttcataca tcgccgcttc accacgggta ggaaatcgcc gccgaaatct
    27661 tcttccttca gatccagcag gccgcacgtc cacacagtat ttgccatcag cgagcttctt
    27721 aatcgacata aggaagcccc ccaatgaaaa catcatcttg atcacaattt tgagaaataa
    27781 aggccagatg gaccgttaac cagtcttctt gccggagtgg ggtgattccg attcgcttcg
    27841 cccaatgtgt gcgagagccg gtaccacctg cccggccttt ggttcgacct catcgaacaa
    27901 aaaccagtca cggtatttac ggaagacggg agacttgagc agcttttttg tggattcaag
    27961 atttggctca tttctcccct gttcatattg acgcagggcc gctaccgtga gccctgtaat
    28021 ttcagccatt ttttcttgtg taagcatttc agacttacgc atgattctaa ttttttcacc
    28081 tacgttagtt gacatagtga gttaactctc ttagtttata catcaacaaa cacacctaga
    28141 gacaatctag gcggctctaa ctggtgctaa atagaaatag caccaataac ggaggatacc
    28201 agatgcaaga agtcactcaa aacgcgcctg aatcgcctgc cgaaacacag tgcgatcagt
    28261 tgacagatgc gccagaaacc gcacaccaaa agaccgggcg caaagcccct gaacgccagg
    28321 aaatcaggct ttcacaaaac ccttctcatc tcctgtcaaa agaagggttt gcgatgtata
    28381 tcggcaagac cgttgacgcc atcgtgagca tggcaaaagc agggaaatta cccgctgtgt
    28441 acatggcgga ccctctcaag cctggcggaa acgccgaact ctacatcaac cggaaagcgt
    28501 gggatgaagc ctgcgatcga ctcactgaaa acgcaccgca agaatggcac gaatgggaaa
    28561 accgactttt tttatttaag ccagcagctg gttcacggaa taaacgcatc agtaaaaaag
    28621 ctgcgtgata tagaactggc aatcattgga ggggcaagat gattaaacac acggaaatca
    28681 gacaaagaat aaaaataagc gacgacgctt cagtaataat ctgtaatcag aacttagatg
    28741 ccattgttcg ggctggcaac gatttgtatt tccattaccc gacccattgc atcgttgtga
    28801 catttgaaac agaagagtcc ttaaaaaatg caatggagca gacaagattt cagaatgaaa
    28861 cagtaataag aaaagacaac tgggcataaa tgccaataca ccgttatttc aattctttta
    28921 acttaagcgg gttttccgat atgacacagc gacgcaactt ccgcactggc acagaacgcc
    28981 acgcagcccg tttctcaacc agtgcatcac gcagcaacac acactacagc ctgcaggaaa
    29041 cgcacgccac acctgacggg catctggtta aacaaaccgg taaacacacc tggctgatta
    29101 atgatgccgg aattgtcatt caccggggcg cacgcaaccc gattaccgga aatacctgtt
    29161 actccctgac tcgcggagat gagcagttcg ggcaggattt caccctgcac gaagcattaa
    29221 gaacggcaga ccggttaatc agcggtcgca gttttcagca cccggaacat cgccgctgat
    29281 aaataaaagg attcagccat gatcgaagcc tacaaaatac ttattaataa tgcgttgcag
    29341 cgttatcact tcgaactcgc aaaccggggt gtaaccaggg acgaaggact tcgtaactca
    29401 ctgcggggcg caattatggg actgtataac tgcgcactgg gcagcgatga ccgcgaagca
    29461 ctggaagaat taaaagttat tgtggccgaa ctgtttgaag gtgaaatcgt tgagccttat
    29521 ttcacagagg tagcagcatg agcgtatttc tttcatgggt gattctgatt atttccgtgc
    29581 ttatggcgat tggcattatg cgaattatcg aagcgctgaa aaatatcgaa tctttattta
    29641 ctggcgaaga acgccgtaaa taaataacca accggacaga aaacattaaa accatccgca
    29701 tctgcggagg gcttcactca atccagaaac ggagaaacag ctatgagcac caatacacct
    29761 acagaaataa acaaactatt taccgacccg gcgcatattg aattgacgca aaaaacgctg
    29821 actgaaataa ccaatgtact ggatgaaaga atatcagaaa tagataaaga caagcatttt
    29881 gctacttaca tggccctgca aatgcaaagc atggcaatgg tgacagcaaa acagaccatc
    29941 agtgaactct atatgaaaaa cctgcgcctc gagcgcgaac tggcagaact ccgggcacag
    30001 tccggccagt tttcagcgtg aggggcagga cgatgaacag catatttacc gccacaagac
    30061 gaaccctgct gacgcatttc accgatgccg caggccgcga gttcatggtg gaaagttatt
    30121 tgatcacaac gaccacgccg tgcccgtcag acgctgatta tctctacatc catctggctg
    30181 acggcacaca aatcaccgcg attaccagta ccgttcgcga agtaatgacc atcagagggg
    30241 catggaaatc agaaacacaa gcccacggag agctgcggcc atgatcagca agtacaccac
    30301 caacgcacgt aaacccggta aagacgcacg ccaccacatc gccgtactcc gcaatctgtt
    30361 actggaggaa atcgacgact tcacggcaac gctgagcggt ccgggcgcac cagtaacacc
    30421 aggtgagatc caccgggacc ttaaacgccg tattgaaaac gccattgatt acaccctgaa
    30481 cccgaccgaa taaccggaac agtaacggag gttatcgcga tgagacttac acctatggga
    30541 ataccgggca aagccccggc acatgtgaag gcgtggacac agcaggaaga tcggttactc
    30601 atctcgcttt atcaggaaca caacacacat cagatggctg aacgaattca gcgaacccgt
    30661 aaagcagtta tgcaccggat tcgtttttta cgtaatcgtg ggctgattgg ccgcaagaga
    30721 aaagcaccac tgagtacaga agccataacc tttcttatta aaaaccgtca tgtaaaaacc
    30781 gcacaggaaa tggctctgga agtgggatgc agtgagtgca cagtgaggta cacactgcgt
    30841 aaacggggtt acaacctgaa aaaatgcggt gagaaccacc attgcgccag atacagcgat
    30901 catctggttg agctggtcac ggagctacgc gacatacaca acatgacgtt ctgcatgatc
    30961 gctgaacaca tcaacagcac gatacagatg cacattaacg acgatgcggc gtttcatctc
    31021 taccaccgcc gcaccgcaac tgacgcagtg ctctgtgaac tgttaccgga ctgagtgatc
    31081 atcatgcaca cacaaaaaaa ccgcttgcca gcgttcgcct ggtcgggtta cagttttccc
    31141 gcacctcata aaacgggtgc cgggatttcc accccgccga taaccaaagc gcacaaccgc
    31201 gccagcggtt tttttgtgcg tactgtattg ccacgtcttt ttcgcgtcag aattatggcg
    31261 gggcgtacgg ggccgacttc ggtcgggccg ggttctttgg ttaccggttg tggaaaccct
    31321 gtacgcctcg ccaccccgag ttttccacct ctggatggtg agttttcaaa attcacaacc
    31381 aaagaggcca caccatggca aaccgcaaac aacagcgcgc ccgcgctgag cgtcttcaca
    31441 tccgcactga aattaaccgc agactccatc gggctgcatc tgtcaccttc atcatgcagt
    31501 caaacgcatt acatcgactt aacgatggta tcgctgccgg gtattacgcg gcagtcttca
    31561 gctatctggc cgaagacctg gcagaactgc aggaccttat caaccagcaa caccaccact
    31621 aaacaacatc ctgaagccat cccgcacccg tgcgcgggag gcttccgcac gtcggttacc
    31681 ggaggttctc atgaagagcg ttaagggact ttcagatatt caggattttt ttaactggat
    31741 gtgggcagtg gatccccggc tggcttcccg cctgaacgac tggcacgact attaccgccg
    31801 cgcctgtaaa caggccgcca gacgcgtcac cacatacggg gatttctcct tcaccgttga
    31861 cgaccgttac cgcgtggacg tgaaaggcga tggcgatggc tttggtttat ttcacatcaa
    31921 cggcaccgac agcaccgcca tcagctggtc gtcgtcagag atggcgcttg tgctggaaat
    31981 gacaacgcac agcgtggccg tgaaaggcat atacaccgca gaagacttta tcggtgaata
    32041 ccagcgcctt ctcgtgctgt accgcgcggc gtttcaggcg gcgactgccg gggagtgcga
    32101 cgcatgagtg accacaatgc acagctggcg tacccgtgga acgccccgct accggttatc
    32161 gatcctgaaa cgttcggcag agcgcaacca accgcattac gccaggccat tcagagctac
    32221 atcaacgaag acattcgcat tgatgcaaga ctggatgaag agaccgtcga cttcctgaca
    32281 aacacagacg aaggcaagcg aattaattcg cattttcacc acgacgaaga gcgtcggatc
    32341 aggctggaaa aactggcgcg gcacaaacac gagaatccgc caacactggt atctgaagcc
    32401 atggcagagc tgcgcgccct tccgccgttt cttgccggtc cgctcatccg tgacctggca
    32461 cgcctgagcc gcaaacagga gagcgcacgc cgggaagggc ttaagaacaa cagcgcatac
    32521 gcggcggata acttcgttcg tcacgggctg cgtgcccgcc tgaaacgtat cgcccgtgtg
    32581 aatgaacgct ttgccacccc ggcctttaag gccaccgccg cgcgcgaacg gctggatgaa
    32641 ctgcttatgc tgccgcagct gagccgtgac gaaatccagc gccttgccac cctgaccgcc
    32701 accgcgtttt ccgccgcact ggagcgcaca tgtgatgagg tcatcgaaaa gaccggcagg
    32761 ggtgacgatg acctgttcac ctggttactg acctaccagc cactggcaca catggcgtta
    32821 aaactgggta tcacgccacc ctgctggccg tcgctggaaa tcaggcccga ccgccgcacc
    32881 gcccccgacc cggaacaggt gccgggtgcg gtcatgcgcc tttcctgtgc gacatggtgg
    32941 aacagtcagt taagacacct ccgggacatc tggcgtgaag agctgttacg cgccgcaggc
    33001 ctggtgtcac gtaagacgtc ggtatatatc agccatgaag cgttactgga ctggcgcgaa
    33061 aaacgccagc gcacccgcga ttttctgaag gcctgggaca tcgagaacga agaaggagaa
    33121 cggttcagcc ttgaggatat ctactgggca ggtctctcta atccgcgtaa ccgccgtaac
    33181 gagatgatgg cctgcgtccg gggaatggag caggtcgccg aagcacgcgg cgacgtggcg
    33241 tttttcgtca ccgtcacagc cccttcccgt tttcacagcg tgaacgatga cggcagtctg
    33301 aacccgaaat actgcggggc aacggtacgt gatgccagtg attatctggt ttaccgcttt
    33361 ttcgccgccg tccgcaaggc catcaacaag gccgggctgg gctggtacgg cgtgcgcacc
    33421 gtggagcccc accacgacgg tacaccgcac tggcacatgc tggtattcac cagcccggaa
    33481 aacgaagccc gtatcactga aatcatgcgc aacgccgcca tcagggagga ccgcgcagag
    33541 ctgggtgatg atatctcccc gcgttttaaa tgcgaaaaaa tcgacccggc aaaaggcaca
    33601 ccggcaagct atatcgcgac ctatatcggt aagaacctgg acgccagcgc cttcatgggg
    33661 aatgacccga aaaccggcaa accttacgtc gataaggaat caggcaaaac catggccgaa
    33721 accgtggaga acgccatcgg ctgggcggcc cttcaccgca tccgccagtt ccagttcttc
    33781 ggtatcccgc cccgccaggt atggcgcgaa ctccgccgcc ttgccgggca gatggcccgt
    33841 aacccgactg ccccgcagcg tctggaccat gacgacattg acgccatact ggccgccgcc
    33901 gacgtgggat gttttgccac ctatatcacc cgccagggtg gtgtgctcat tccccgcaac
    33961 acgtacctgg tccgcaccgc atacgaaact gccgaagagg ccaacgacta cggcgaattc
    34021 ccgcaacgca tttacggcgt ccgcgccccg tcactcgggg agcgctacac catatgcacc
    34081 cacccggaca cctggaaact ggtcagacgt aagccggaaa acgaagaccg cacgaacgag
    34141 ggttttgacg ttgagtgcgg ctttgccgca ccttggactc gtggcaataa ctgtccccct
    34201 gaccagaatc tgaccataaa cgacccgcca gaaaggcagc cggacggacg cagggagcag
    34261 cgacttaccc tgccggaagg tgatttgggt gaatggttaa ggtcactgac ccccacagag
    34321 cgaaaacaac tcaccaggca gctgaaaaac ccgccacctg acacggaaac cgtgaaaaac
    34381 ggcggcatca gtgcgcccgc tggcggcgtt cgcgtgagtg tcgcactgac cgctgagcca
    34441 cacatcaccg cacaaatcac ccgtgagctt gatgcgctgg gcgtgcagct gcatgaaagc
    34501 gccgtcgttt ccgtccggca cggtgcccgc gtgcgcctgg ctgacggacg caccgcgtac
    34561 tggaatgaca gcacccgccg cattgatatc acccccgcac cgaatacgga ggcgccacaa
    34621 tgacccgctt ttattgcgcc atgcagcgac agcgttttga cgaaatgatg cgccagcgca
    34681 cgcccttact gaccctgaac aacattgcca cggggccaca accgggcgat gagctggaaa
    34741 tcacggtcgt cagtcccgat cgcaccacca cggccatcat caccgacata ctcaacggac
    34801 gaccggacaa cactaaccgc agctggcttg tcgccgttcg tccgcgccgt ccgccagggg
    34861 agccggagcc gctcacgcct gccggaaaaa tcatgcggca gtaccacgaa gagcaggcac
    34921 acaaacacaa cgcagaactc accagaacac tggtcacaat taaccgcaaa gcggcaattg
    34981 cccgctatgt gcattcagcc gctggcagac aatcacgcag gtaaacaaag accatgaaca
    35041 taaccagaaa cagccaacta aaggagcgca ccatgaacga caccaggagg atcagcgaca
    35101 gggagcttac gaaaatgatt gagtcggccc ggcaactgct ttcacgacaa aacccggtcg
    35161 tggaagaaac gtggtgggag aatttacttg cagcactgac cgaactccgg gaacgccgga
    35221 acaacgaagg agccgccaaa tgaagacccc aggaatgacc ggaaaggaaa tcattttacg
    35281 ctacctggaa acgcacacac gtttcagcgc cccagaactg gtagaaaaac tgggcgtgaa
    35341 aagaaccgtc attaatcagg ccgccgggaa aatggtacgg gaaggcctgc tggttgtgga
    35401 tggatacacg gacaagataa cgcattaccg gaagccaacg gaaaaggaac gacaggaact
    35461 ggcgcaacgg gctgaacggc agcaggaaaa atcagtcatc gaagcctgca agcgcagcga
    35521 aatcatgaag cgaattctgt ttatctacgg cgctgcattc cgttaaagga gctggtgagt
    35581 cggggcaaaa attctttttg ccccggcgaa gcagcagaca agcgaagcgc gtcaggaacg
    35641 ccgcgacagt agttcaagcg cataccggcg atcttccggg cgcatggcat caacaagaaa
    35701 cttcaccata ttgcgaccgg agcgggcgcc ggggctgagg gtatgggaaa aggtcatcgt
    35761 acagacgaag gtatgcccgc attcgacatc actgcatgca cagtacagat ccgcaagttc
    35821 aggtgttttc cagtctgtct tccggatgac agcaggtgcg ccacagtccg gacaccgggc
    35881 tttaaaaatc cgcatcttac ctcccgataa cacgtatccc ggggaggaca ttttacccga
    35941 ccggcaccgc tttttcttcc gccacatgcc cggcatccac atcaaaacaa agataaaggt
    36001 gttccggtat atcgggatcg ctgttcacgg cgttcatgat tttacgctga agcggtatca
    36061 cctcatcctt gcggtacgtt tcacgcgtcg tttccgggtt agggatggtg gccccgttcg
    36121 tggggatgat ccccgcaaga ccggcaggaa agcggtgtgc cgtaaacaca tcctgggcgc
    36181 tgatcccctt gatgtttgaa aattcatcct tggcgctcac ctcaccaatc ggaataattt
    36241 tcaccccatc cggcgaacct ttggggatgt tgatgtacag attgcggaaa tttcccagcc
    36301 ccttagactc cgccaccttc tcgcggatct catcctccat ctccgtggtg atcccagggt
    36361 cgttcgtata cagcaaaaac cccatatgcg ccccgttgtt gtagtaacgg cgacgaaaaa
    36421 ggctggcctc gctgttcagc agcgccgagt gaatgccacc gatataatcc ggcagcccgt
    36481 aaatttgctg acgcggatca tacatccgca gaaagataat atcttccggc gtatagacca
    36541 gcgcgggacc ttcctgaagc accacgaact caccggtttt gcggcgccgg agatacagcg
    36601 acggaagcgg ctcaagtgcc acgacgtcgc cgaatacgtt acgcaccttg agcagggcga
    36661 gatcgccgaa caacaggtag tcaaataccg cctgttccat ctggtccgtt gtgaggccac
    36721 caccggtata gccccccgcc accatgttac ggcgggcata cagcacccca ccgtgctggg
    36781 cgttaaggtt cgggagctgc gccagtgcca ggcggttaat cggcagctgc cagtggttgt
    36841 gttcgttgtc ataccagatg ttatggtagt ccgtgccggt ggtcagcacc ggttccggct
    36901 gaccaaacgt gattgtgctg ccacgtgcgg gcgtgaatgc ttttttcgtc acgcgtgacg
    36961 ttttgtttct gcgtttcttg ctcatgcggc tttcccgaag gcccatgtgg acgggcgttc
    37021 atagtcataa tctacaggct cattaatcac ggcgtgcgaa atggcaaaaa acacatccgc
    37081 atgccccgta acatccgacc gttccgccac gaacgtcagc gcgttaccgc tttttgtggt
    37141 ggtgcggcgt atggacatga acgacgcggg gatctccgtg cgttcacggc tggcctcatc
    37201 aatggcgtct ttcgcccact cgatacgctt atgggccacc acgtctatca ttttcatcac
    37261 caggcggttt ttgctctcca cgctgtaaag aatggcgtta gcctcacgtg gcgcaaagcg
    37321 actgacgata tcaaacaccc ctttcccgat accggtgata tctatgccga tataggtaat
    37381 gttaaagcgc tgcatcagtt cgcggatctg atcggcctgc caggtgaagt taaatccctg
    37441 ccactgccag acagcgagca cacggaaccg ctcgccctca tggacaggcg gggccacaat
    37501 cacaaacgtg gaattatccc cggaccttga cgggtcaaag cccgcccaca cctcacgatt
    37561 gccgaacgga cgtgctgccg tgggatcgta gtcaccccac gtctccatct ccacctcgca
    37621 cctgaccagt tcggagaact taaataccgc atccttgctg tcaacaaact cgcagccgta
    37681 cagcatcgcg taagtggtcg gattgtagcg gttcctgata cgttcaatat caacccgagc
    37741 agacaggcca cccgccaccg cctcttccag ccggataatg tagcgccaga tcccgtcagg
    37801 gcattcacag cccacacgca tcgcgctttc tttcggaaac tcaacagcac gacgcgccgg
    37861 gtcatccccg cgccagtcgt caccattcca gaccagataa gcctggtggg ttttggcgct
    37921 gggtgtggaa aaatacgtcg tgcggtattt gtcgtgggtc gccattgccg aggccacttc
    37981 atgcagtcgc gtgaattttg ggatccagaa cacctcatcg ccgtaaaggt ggccgttaaa
    38041 tccctgggcg gtactggcat tggtggacag gaaacgcagc attgcaccat tgctcagacg
    38101 gatatgctta cccgtgagcg tgatcccgaa ctgcagggcg aagttaataa tatattcacg
    38161 gaagatttcc gcctgtaccc gtgacgcaga aaagaaaatc tggttatcac cggtcaccac
    38221 cgcatcttcg aacgcctccc atgcaaaata ccaggtcatg ccgatctggc ggctcttgag
    38281 gatgaaacgg cgctcacggt gccggttctc gcggcagtgc agctggtagg cataaagatt
    38341 tttacgcgcc cattcatcca gcatttcagc agtcagtccg gaaacgtcgt ttttccggta
    38401 gcgccgcttt ttcccttctc ccccgccgca ggtccccgca gccacgccgt cctcatgccc
    38461 tgccggacgc agggcggcaa tttccgccat cctttcagcg tgtttgtttt tctgggcgat
    38521 gatcttcacg tgctgcgcga taagttcacg cagttcggca agttcacact ctgtcttgcc
    38581 ctcccgtccg gtcagcacat caatacgccg ggcgatcacg tcctcaacgg attcagcggg
    38641 cagcagggta gcccactgcc ccacatcaca ccagtggtaa accgtgcgcg gcggaatacc
    38701 gagttccgcc gcaatatcgc gggggtgcca ccgcttcagg taaagcgatc gcgccgcctc
    38761 tttgatttcg tcagaatatt tagccatgcg cccattatgg cggcgcatcc ggcccgcatt
    38821 caggcagaaa atttcggctg tgttcggcta tcccgtgtta tccgaatgca accgaattaa
    38881 tccagtagcc ccccgctgta aaaatcccga cactcaccgg agttcttcag attttcacgt
    38941 aaaagaggcc acgatgccgc agccaacgtt gatgaccgac tggatatgta tcgccacatc
    39001 aggaccgacc gttgacggcc gcgaaattga tccgcagtgg ctgactgatg ccgctgaaac
    39061 ctattcccgc aacacctaca cggcgatgct gtggccctgg catgaggaag aaatcggaca
    39121 ccgccagtac accagtaatc tgggtgaagt ggactcactg aaagcggaaa ccggcgagga
    39181 cggtaaaacc cgcctctacg cacggctggt ccctaaccag tacctgattg aagccaacag
    39241 actggggcag aagctgttca catccgccga gatagtggag aacttcgccc ggagcggacg
    39301 cgactatctc ctgggcgtgg cggtgacgga tatccccgcc agtctgggga cggaaaaaat
    39361 acgcttcacc ctgaacaacg aggaatacac ggcacagcgg gcgaactgcc agacgttcac
    39421 gctgggcagc ctgtcaccgg acacctcccc ggccccccgt aaacctgaat cattcctgaa
    39481 acgcctgttc agcgcaggac agaccgacaa caccgacacc agtaagcctg atacaggcga
    39541 ggacgcaaaa atggacgaaa ttaaagcact gcttgaaaag ctgatcaccc tggtggaagg
    39601 cggcagcgcc gctgccaccg gcgagggtac agcagacacg ccggaagaag caaaagccga
    39661 agtcgcggaa atcgccgccg aaatcgccga tgccgccgca gaggtggcag agctggcaga
    39721 agaagtgaaa gccgccccgg aagacgaggt taaggccgaa gcctttaccg ctgcaaaagc
    39781 gaagctggaa gacgccatca aaaccttcag caccatcaca ccggaaaaag gcgaacgtta
    39841 ccgccgtttt gccgcccgtc gccgggacga caccgacaaa gaagaaatca aagccattgc
    39901 cgaaaaactt aacgacctga ccacgaagct gtcagcaacc ggcatcacgc cgcgcccggc
    39961 tggcgcaccg gcagaaagcg aaaaaccgtt tgactttgtt taaggatacg caacatgcaa
    40021 ctgaaccaga aaaccgaatc actgcttcgt aagtacgctg ccgggctggg cgcggcaaac
    40081 ggggttgacg acgtcacacg ctattttgcg ctgtctgacc cgaaggagac cgcgctgcgt
    40141 gacgccatca tgcactccga cgagttttta tcaaaactgc cgaacgtgct ggatgttgaa
    40201 caggtgacag gccaggtggt taccaccggc gttccgggcc tccataccgg acgtaaaaaa
    40261 aatgcccgct tcacccttcc gctgggtgtt accgggaatg aatacaagct ggctgaaacc
    40321 gattccggtg cccacctcct gtacaccaca ctggtgacct gggcaaacgc aggcggtgaa
    40381 aacgagttct ttaaccgcat tcagaatttt gttaacagac agatcgcgct ggatatcctg
    40441 cgcatcgcct ttaacggtac cagcatggcc gaagataccg acccggtaac caacaaaaac
    40501 ggcgaggacg tcaacaccgg ctggcatgcc atcgtcaaag gccgcagcgc aaaacagatc
    40561 gtggaggctg gcccgacgct gggcaagggt ggcgacttta tcgggctgga tgcagccgtg
    40621 gcagacctcg tgcacaccct cattgatgag tcgtatcgcg gtcatccgga tctggtggta
    40681 ctggttgcgc ccgatcttgt ggcacaggac aaggtgacgc tgcttaacgc catcgacgcc
    40741 cccacggaaa aagtggccgc gcagcttatc ggtcgccaga tcgccgggct gacagcatac
    40801 accccgccgt tcatgccgga aggacgcctg attgtcacca cgctggcaaa ccttcacatc
    40861 tacacgcagc gcggcacccg tcagcgccgt gcggagtgga acgacgatcg caagcgtttt
    40921 gaaaacaact atctgcgcat ggaaggttac gccgtggagc acgacgaaat ttacgcggcc
    40981 tacgacaagc tgaccatcaa gggcgcagaa gaagccgaag acgtcggagc ataagcacag
    41041 agggcggcgc aatgacaccc tgccagcgac accgggcgaa aatccgcaca caggaggcac
    41101 tcgaacgccg ggaggcgctg acggcctccc cggtcagctt ccacctgtta cgcgcggagc
    41161 ttgatcgcga tgtggcacgg ctgcgcagtc ttccggtccg tgaagaacgg ctggcgttca
    41221 agcgcgacat tctcctgccg cgctggctgc ccgtcgcgga gcgctatatc gctgacggaa
    41281 agcgccacgc ctgcccggta ctggtgtact gcatcatctg gctgttcgac acgggcgacc
    41341 tgagtcgcgc ccttgactgg gcagacattg ccatcagtga aggccaggcc acgccggaca
    41401 acttccgtag caccctgccc gccttcgtgg ccgacacggt gctgcagtgg gccaccgaca
    41461 acagcgaggc cggacacagc atagagccgt atttttcgcg caccttcagg aacgtcacca
    41521 ccacgtggcg gctgcatgag gaaatccgcg cgaagtggtt cagatttgcc gggctgtatc
    41581 ttctgcgcga cgaggcgggc aaaccgagag ccaccgcgat taatgatact gccgtactgg
    41641 aaaaggcaga tgcatacctg gcacaggcgg cagcactgag ccgtaccgct ggcgtaaaaa
    41701 gtatcagggc gcgcatcagg gcacgcatca gcgcccttaa tgcagcgtaa acgactaccc
    41761 gcagggccgg gcgggcgcgg gaggaggcat cagtgacgga aactgtatgg ccgtggaatc
    41821 ccggacagcc cgccttttta ttcatatgag gcacgtcatg agcgaaccga gcttcagcgt
    41881 cagcggtaaa ccgctgaaat ccacaccgca gacggtgaca aacggcgagc cgttctggcc
    41941 ggaccttgat ctggctgagc ttcaggggtc acgcacgctt ccggctgatt tacccgacga
    42001 cacggcggcc atggcgctgc ttgccgcagt ggccgaggtg aacaccacgc tggcgccggt
    42061 tgtggcgcac tggaaggcgc aggggcatga acgcgccgca gacgtaccgg gggccaggct
    42121 gggcgatgaa acccagctgg tcgcacagta caaaaaggcc gtttatgccc gtgccaaggc
    42181 cgatcttatg ggcgagttcg ccaccattgg tcgccgtgaa acctatccgg ggcaggaaag
    42241 cgaggacaca cgcgccacct tactggctga agcctcgctg gtcatgcgta acatgctggg
    42301 gcacagacgc gcgggagtgc acatcatatg aaccagctgg aaagcctgac cgccttcatt
    42361 accgaaaatc tgccgcctga cgccatgcag ttcttcatga gcagtcagga agacgcgcag
    42421 cttatccgca gtcagaaggc gctgggaaac gaccagatcc gcatgggcgt gatgacgtac
    42481 accggcgtga ttgcatggga taacttcccg ttccgcaaat actcaccggg gctggtttac
    42541 gcgctggtca tggcctggct ggaagagcac gcggacccgc tgagggagga attccgcccg
    42601 gaggatgtga acgtcgaccc gcagtttaac gatgaggact cgtgccttct cgatatcacg
    42661 gtgggactgg ctgacccgct gatcatccgg gtgcaggaag acggtcccgt tccgtacagg
    42721 gggaaacgct ggacactggt taaccctgaa atctggacgg cggaaaccat ggatctgttt
    42781 gtcagcagga gccgcgaatg ataagcggcc agctgaaccc gcagcagctg gcagaagcgc
    42841 aggcgatgct tcagtcgctg gatctgccac cgaaaaaacg ccgtcgcctg ttatggcgta
    42901 ttgcccggca cggcattatc gccgctgcca gacgccatca gcgcgatcag gccgcaccgg
    42961 acggtacagc atgggcaccc aggaaacgcg gtaaggggaa aatgttcaga aagctgccgc
    43021 gtcttctggc ggtccgtgag atgcccggac aggatgccgt aaaggtttat ttccgtaacg
    43081 gcaatcacac cagtggcagg agccgcatca gtgccggtgc cgtgggctgg gttcaccaca
    43141 acggcgcaga aatccgggtc agtgctgaca gcttcagcgg tcaggaccag aacgggcaga
    43201 ccgccacccg ccggcaggca aaacgactgc gggagctggg ctttaaggta cgccagaagg
    43261 ggcgctggcg gaaagcatcc atgaaatacc tgttaacgct ttcgcgggcg caggccgggt
    43321 tgctgatcac cattatggaa gatcagatta tcgccggacc acgccagcag aaggcccgga
    43381 catggaccat tacccttccg tcgcgcgtct ttctgggggt aacaaacgaa gaattttcac
    43441 acatcctggc acgccagatg aacgcaatgg gctacggcct ggacgttgac gcacaggata
    43501 tcagataagg agtcacaccg tgagctggcc gaatgtaacc gtaagccatc agaaccgttt
    43561 caacggtgcc acccgtgaag tggagcgcac gctgcttttt gtgggctatg gcaaaaagaa
    43621 cacaggtaac accctttccg tctcccctga gaccgacctg gacgacgtgc tgggaccgga
    43681 tgaaagcctg cttaaatcca cgcttaccgc cgcaatcgcc aacggtggcc agaactggtt
    43741 tgcctatgtt cacgttctgt ctgaacccaa acccccggca ccggagggtg gcgacgctaa
    43801 cgccgcatgg gtggacgcgg tgaaaaaagc gcagaccatt gcttctgccg agggcgtggt
    43861 gattgccatt gacatcacgg ccaaagatgc cgttaaccgc gccaccgaaa cccgcgccct
    43921 gcttcagtcc gcttatggcc gctttgtctg gttcatgctg tgcgtggccg gaccgggcaa
    43981 ggatgaagca tgggcggcat acgtcacccg catcagtgcc atacaggacg gcgtggcagc
    44041 acctggcgtg atggtggttc cccgcttatg gggaaatgag cccggcgtac tggcgggccg
    44101 tctctgtaat ccttccgtca ccgttgcgga cagtccggca cgtgtggcaa ccggtgccct
    44161 ggtggcgatg ggtaacgatg agatcccgca ggacggcaag aaacagcccc ttgagctggc
    44221 cacgctgcgc tcgctggaag gcaaacgctt cagcgtgccc atgtggtatc acgatttcga
    44281 cgggctctac tggtcagacg gacgcaccct tgatgtggag gggggcgatt accaggtcat
    44341 cgaaaacgtg cgcgtggtgg ataaggtctc ccgccgtgta cgtctgcgcg ccattgcccg
    44401 ccttgcggat cgcacactca acagcacacc gggcagcatt gccgccagcc agacatattt
    44461 cagccgcacg ctgcgtgaaa tggcgctcgc cacggagatt aacggcgtac cgttccccgg
    44521 cgaggttaaa ccgccacagg acggggatat caccatcgtc tggaccagca gtacacacgt
    44581 gcagatttac attgtggtac gcccctacga gagcgcaaaa gccattggcg taagtattga
    44641 actcgacaca gcactggaga tctgacatgg ccggaaatga acgtatcagc ggcggcgcgt
    44701 ttgatatcaa ctacgacggg atcatgatcc acgtggaaag cgccaccgtc accatcaccg
    44761 acaacagcgc cgtggcacag acgcgcggcg tgccgaacgg ctacacccgt ggttctgttt
    44821 ctgccgatgt ggaggtggaa gtcgacgcgc agaactttaa aaagtttggt gccgtggccc
    44881 gctctgcggg gtcgtggcgt gccattcctg aaaaggattt tctcttttat gccaacaacg
    44941 gcagcgagga ggaaaaaatc gaggtgttcg gctgcgtccc catgttgtcg gacgtggtga
    45001 acatcagcgc aggtgaagcc ggaaagacca cgaaaaaaat caagttcatg gtcacaagcc
    45061 cggactttat cagcattgac ggcgtgccct acctgtcagc ccgtgacacc cgcgatctga
    45121 aggggtgatg ccatgccgga cggtcagact tcactcctgg caaaactgat tatcatcggt
    45181 gctgccatcg ggctggggcg tctcatggtg agccgcgatc gcatcacagc ccgtctgctt
    45241 atcgggcgaa tgattatggg ctctgccgtg gcaccggttg ccgccattcc gctgtttaaa
    45301 tttccggaca tgccggagct ggttgtggtc ggtctggcgt gcgcgctggg cattatgggc
    45361 agcgccttta ttgaagaatg cttttgccgt ctggcagacc gctacctgaa gcagaaggaa
    45421 cgcagaaatg acccataagc cttacatttc gcctttcctg agcctgtatc acgcgcaggc
    45481 gcatgacgtt ctgcgaatgc tgccggaaaa cagtgtggac gcgctcatca cagacccgcc
    45541 atacagttca ggtgccacac acaaggcggg gcggacatgc cagggcagcc acgccaaata
    45601 tctcaacgat gaagatcttc accgctttga aggcttcgaa ggcgagaaca tggacgcccg
    45661 ttcatgggaa tactggacgc atctgtggat gacacaggca tggcgcacgg tacgacccgg
    45721 tggttacgcc ctggtcttca ccgactggcg gcagttgccc gcgctgacgg acgcattcca
    45781 ggccgccggt tttacctggc gggggatcgt ggcatggaac aagggacggg cctcaagaac
    45841 accgcatacc ggttattttc gtcaccagtg cgaatatgtg gtctggggca gtaaaggcgc
    45901 gctgggcaaa tccccttccg ggccgttcga cggctgcatc acgcaccaca cacgcccaca
    45961 ggagaaacag cacccgacag gtaaaccgga atcgttgatg gatgagcttg tgcgcgtgtg
    46021 tgaacccgga gggctgatcc tcgatccgtt tatggggtcc ggcaccacgg gcgtggccgc
    46081 ccttcgccgg ggctgccgtt ttacgggtat tgaactgaac gcccactatt tcagcgtggc
    46141 acaggcgcgc cttacgggga cggaacagag gaatgactga tgaaactgag cgaaaaacaa
    46201 caactgtttg ccgtcatgat tgccgatctc atccactggg cacaggagca cggctatcgc
    46261 ctgaccttcg gcgaggcata ccgcacgccg gagcaggccg cactgaatgc gaaaagcgga
    46321 aaaggcattc gcaacagcct tcacacgctg cgcctcgcgg tggacttcaa cctgttcatt
    46381 aacggcgaat accaggccga caccgacgcc tacaggccgc tgggggaata ctgggaatcc
    46441 atcggcggca catggggtgg acgcttcagc cgggcagacg gcaatcattt cagccttgag
    46501 cacaacgggg tgaaatgatg aaacagctgg cgggctgcct ggtgatcctc tttgccgcca
    46561 tgtgggcggg ctggctggcc catgactggc atgacgccag ggtggcgctt gccgaacagg
    46621 aagccatgga agccacgcga cgtgcggtaa cagaagccgg gcagcagtcc gcccgcatac
    46681 tggaaaaacg gctggaggcg ttaaagggca atgaaattca cactgaacgc gttattcaca
    46741 cggaaaccgt ccgcccggta ttccgcaatg tgtgcgctac tgatgattac gtccggctgt
    46801 tcaacgcccg tgctgaagcc gctgaacgta ccctggcagg aaaaccagct gacggaatgc
    46861 ccgaagacac tgccgagact ggcaggcccg acaggcgctg attttgatgc ggcactgcga
    46921 accctcacga gccgctacac cgaatgcgcc gccaggcata acagcctggt ggaaaccata
    46981 cgaaacacgg agaaactgca atgaccacga agagcaccgg caccatcacc ctgaccgtcg
    47041 cgggcgtaac cctgaccttt gagccgaaca agacggcatt taacaacctc atcaacgaaa
    47101 tcacgatgac caacaagata gcgccaatga ccacctatct taaccgtatc gtgaccgctg
    47161 actgccggga tgcactggcc gacctcatgg atcgttaccc cggctgcgag atgcagatcg
    47221 ccgagaagat taacgaggtt tactccccga aactggagat tgagttaaaa aactgaccgc
    47281 gcgggtcgaa agcatccgcc gcaatcccct tgagcagtat ctggcattgc ggcgtcacta
    47341 cctccccgga gagccggacg acgaagagag catcgcccgt gccatctggc tggatgaata
    47401 ttttgcccgt acccgcgcca acaagaccgc tgaaggtata gcgatcgcat ttaacggaag
    47461 gtgacatggc cagccagctg gatttcatta tcaatctgac agaccagctt acccgcccgc
    47521 tgcggcaggt tcaggaatcc gtgacgggat tcgccgaacg ctcgcagggc gcgttcgcgc
    47581 gtatcggtgt gggtgtggcc ggactgtggg gcgtggccga aggtatccgc gccgcgatgg
    47641 atccggcgtg ggagatgcag aaggcgctgg gcgagcttga ggccaaaggc gtaggatcgc
    47701 aggcgctgac cgccctcacc acgcaggcca tgaaactggg gaccgcattc ggtcagtctt
    47761 ccacggagat catccgttcc gcatggcata ttcgcggcgc actggacggg ctgagcgacg
    47821 gggatttacc ccgtgtgacg gcagcaacca gtaccctggc cgtggctgcc cgcgtcagca
    47881 gcgaagaggc gagcggctac cttgccgcca tgggtgcccg tttccgcagt gagttttctg
    47941 ccatgggtgc cgtggggttt gctgaagatc tggcaggtaa aaccgcgtgg atggttcagc
    48001 agttcggcgt ggaaatgagc accatgcagg agcttattga gggcaccaaa aacgcgggcg
    48061 cggattttgg cgtcagcatg gcagagcagt ttgccatact gggcaccctg tcacggacca
    48121 tgggcaccga ggcatcaggc atctatgagg agttcctgcg cagtgccccc gctgccgcac
    48181 aaaaaatggg gatgagtttt gtcgatgcca ccgggaagat tttacccatg ggtgacatcc
    48241 tcgaccgtct gcaggcgagg ttcggcagga acatcgaggg taacgtggcg gcacaacagg
    48301 cgctggatga cgccttcggc agtggcgcgg acgtgattaa aaaactctgg ggacagcagg
    48361 aaatgcttaa ccggagcata tcagcgctgg ggcgtaatga cggcatgaag cgtgccacgg
    48421 aaatggcgga agccatgacc gacccgtggg accgccttgt ggcctccttc tacaatatcc
    48481 gggccgccat cggtaacgcc ctctacccca ttctcgcacc actggctggc cgttttgccg
    48541 atatcggcgc gacgttcgtg cgctggctga acatgttccc gaacatcgcc cgctggctgg
    48601 gttacatcac gctgaccatg ctggcgctgg gcgcagccgg tgcggtcacg aacatcgtgc
    48661 tgggtgtgtt cagcttcatc atggaaggcc ataaggccat cataaagggc gccagggcag
    48721 cctggagtct gtacacactc gcactcacaa aagtacgcag cgccatcatt gccaccagtc
    48781 ttgccgcgcg tctggcgggc gtgtcgtttc tgaccatgag cgccccggtc ctcctggttg
    48841 ccgccgccat cgccgctctg gtggtggtgg ccattaagtt ctggcagccc gtcaaagcct
    48901 ttattcgcgg ctttattgat ggttttaccg gcgcactgga cgccatgagc ccggtgactg
    48961 ccgcgttcgg cgacattgcc cgtgcgctga ccccggtatg gcagggcata aaaaccctgt
    49021 tcggtgcctt ctccgatctg tttgcaccgg tacagctgac cacgggacag atgaacagcg
    49081 tcacccgtgc cggggaaaca tgcggtcgcg tggtggccgc agcggtaagc ctgatatccc
    49141 ccgcgctgga ggtggttatc agcgtgatca gaaccctggt cgacatcttc gccatcgtca
    49201 tcagagggtg gcaggacgtg gtggctgcat ttgacccgac tgccccggtc gaatccttcc
    49261 ggaaaatggc ggggatcatc ggtaacgtct tcggggactt atggaacgtc attaaaaact
    49321 cgtttaaccg gacgtggaac tggcttgcgg aaaagctgaa caaaatcccc ggtatcagta
    49381 tcgacatgaa gcccacggag caggtgaaca ccatgacgcg gggcgcaaca ggcagcaccg
    49441 tacctccgca gattgcagaa accgccgtcc cggcactggc aggcaaccgc atcagcgccg
    49501 agctgccacc cggaggcata cgcaaccaga tacgcacgga cacctcaacc cgtatcgaca
    49561 acagccggac gttcggcaat ctgacaatca acacgcagaa cccgatgacg ccggggcaac
    49621 tggatgaatg gacggagctt tacgcgggat gaacacgcca tcgctttaca tcgacctgct
    49681 tatcactgac ggcagtttta cgctgaacag cggaaacgag ccacagcgat gtgataaccg
    49741 ggtcagcatc ggccaggacg tggtgcacag cattatcgaa agcgggatcg ccgccctgct
    49801 tatcggcgaa cgcagcccga cgctgcgcag cgacatcatt acccggctta tcctcctggt
    49861 tgaggatgat gaacggctgg ttccggggac ggtgttcatt aacgaagagg caccgggcag
    49921 gctgcatgtg acggcagaga cacaggattt tggacgcata aacaccaggg tgaactatga
    49981 aagggaaacc ggacgttgat tttaatcagg tattccgtga aagcggtatg ccggaaacag
    50041 aagaagagat tgccaccctc tttcatgagg atgtacagaa aaccggactc atcaccaaca
    50101 cctcgcccat gagtcccttc tggcggctca ttctgatcat tgtcatacga cccgttcagt
    50161 ggctgcgtga tgcgctggtt gacgttgtgc tgcccggtat gtttgtcgcc acggctgacc
    50221 ggtcacttct tctgcttcat gcgcacgacg tcaacatcac cccaaaaccg gccagtaagg
    50281 cccgtggcgt gatctggttt gccaaaacca gggcgaatga tgatgttctc atccccgaag
    50341 gcaccatcgt tcagaccgcc agaatcaacg gcgtgatata cagcatggcc gtcacggagg
    50401 aaacgcttat cagcagcggt aaaagcggcg catccgttcc cgtggaagcc accaccgaag
    50461 gcagcgggca caacctcgcg ccggggtatt ttcgtatcct tcccgttgcc attcccggca
    50521 ttgcatccgt ggtgaaccgg gaggactggc tgaccgtacc gggtgccgat gaggaaagcg
    50581 acgacgaact gcgcgacaga atacgcaacc agtttaacct cgtgagcagt taccacacgg
    50641 acgccgtcta ccggggactt attgccagcg tggcgggcct gagcatcgac cggatctact
    50701 ttcagcacga cgccccgaga ggaccgggaa cggccaacgc ctacctgtta ctggacagcg
    50761 gcgagacctc tgcccctttc attgaagcag tcaacagcta catcaacgaa gaggggcacc
    50821 acggacacgg ggatgatttg cagtgtttcg ccatgccgga gacgcgtcac cgtctggagg
    50881 tcaccgtata catcagaagt tcaaccaccc tgccaccgga cggcgcggaa gggctgaaac
    50941 aggatatcag cgacctgata cgctgcgctt tccgggagaa cgcccgcttt aacgtgaaaa
    51001 aagtctggcc ctattcgcgc ttttcgtttt ccacgctgag caaggaaatt catcagacct
    51061 tcccggtggt ggcttcggtt cagttttcac tggaagacat catcagcgat ctggccgtgc
    51121 cacggctcga atccctgaca gtggaggtgg tcattgaatg actttttgca gcggcttcgc
    51181 tcgctgaaac tgccctcatg gatggacagg ggagaacccg acaaactgct gcgggccgcc
    51241 cgggcgttct ggtcccgcgt atgggaatgg atcacctggc ccgtgcgcca gtttgacccc
    51301 atgacatgtg cagagcccgt acttgattta ctggcatggg agcgggacat cacccgtttt
    51361 aaaggtgaac ccacatcact gtaccgccgc cgtatcgccc acgcctttgt caatgcacag
    51421 gacgccggag aggtggcagg atttattgcc atttttgaac ggctgggcat tggacatgtg
    51481 gagttgcagg agcgcaagcc cggcgtggac tgggatgtga tcctggtgca ggtgacagac
    51541 agccagatag ccgacaacag cgatcttctt ctggagatca tccgcaaata tggcaggaca
    51601 tgccgccgtt accgttttga ggtggaaacc cttaacccct tcatcatcca ttatggcgca
    51661 tacgaagggg agtatatctg ctggacggcc agggcagagt gaatgactga aggagtatga
    51721 ctttatggcg aaatctgtaa ttaccagcgc ctttatccgg ctcaaggcac agcaggcggc
    51781 ggaaggcacc cccgttgtgc tggatgagtt tatttttgca aacgtcccgg acctcaaccc
    51841 ggaaacaccg gtatcccccg acgaaactct gccacctgaa gatcagattg ttcaccgcca
    51901 ggccgttggc aaaaccggcg tggtgaatga gaatgccgtg gtttattcgg tcaccctggg
    51961 ggcagagctg ggtgattttg aatttaactg ggtgggactg gtcagcaaag caaccagcac
    52021 gctggccatg attgttcacg cccccacaca gaaaaaaatc agaacccaca acggtaacga
    52081 aggtaatgtg ctggtccgtt ccatgctcat ggagtacagc ggtgcgaagg aagccacagg
    52141 tatcaccacc ccggcgcaga cctggcagat agacttcacg gcacgcctgg catccatgga
    52201 cgaacgccag cgccgcgaaa acgtcgatat ctacgggaag gcaacttttt ttgatgacgg
    52261 gtggctggtt aagaaaggcg aaacctggac agtcaacaaa ggggtgggtt acgtggaggg
    52321 gctacgcgca gagctgagca ctcaccaccc catgaccacg cccgccttcc cgtcccgtat
    52381 ctggctgaat gtgtggtgga ccggcacgct caccagtgaa tgggcgacag agcacgaaat
    52441 cataacagcc gacccggacg ttacggaaga gcgtttacgg aaaacgtatc cgagggacgg
    52501 cattcagcaa tatttttgtg ccatcgctga aattgatgaa gacggcaacg tgactgacct
    52561 ccgccccaga ggaaccccgg aagagcgccg cagtctggac gccctgaaaa aacacgagaa
    52621 atcacgcaac cacccggacg caacgctgaa agaaaagggc ttcacacagt taagcagcgc
    52681 caccaccagt acatcagaga cacaggcagc cacgccaaag gctataaaga tcgccatgga
    52741 caacgccgat ggtcggcttg cgaaaaaccg caatggtgag gacatcccca acaaggagct
    52801 gttcctgatc aacgtgggcg caccacgcgt ttatgcaaaa gacgggtacg tgggtgaaac
    52861 agggcaacgc tggacaacag aacaattcgt ggaatggctg aatgaacgtg gcgccttcag
    52921 accaggactc tggatattca aagccaatat ttcactttcc cggcacagcg tgctcactga
    52981 tacgggctat ggtgatatct gccttggagg ctgccttatc gaagtatcag gcgacatcaa
    53041 cggggggatc atcaggatca cgacaggtct cgaaagccca aaccctgccg acatgtacct
    53101 ccgcacacag ttcaccgggg ttgttttaaa taaagggaaa agcaccatct ggtaccgcac
    53161 ttacacggac aaaaacaaac ccacaccaca ggacatcggc gcactgcctg caagtgagtt
    53221 gttacccgca ggcgttcccc tgccgtggcc gtcagacacc ccgcctgacg gttacgccat
    53281 catggtgggg cagacattcg ataaagcgca atatcccctg ctggcaaaag cctatccgga
    53341 cggtcgcatc ccggatatgc gcggctggac aatcaagggg aaacctgcca gcggtcgtgc
    53401 ggtattgtca caggaacagg acggcatcaa atcgcacacc cacggaggga gcgtctcaaa
    53461 tactgacctt ggcacgaaaa cgacctcatc atttgactac ggaaccaaaa caacctctac
    53521 aaacggacaa catgaacaca gcgtccccct gagaagacaa gatggcggag gcataaactt
    53581 tgactggctg gatggttcat cgtccggttc tcatattggt aatggatacg ttcctgccgc
    53641 aggtgaccac caacataccg tacccattgg cgcacacacc cacacgctcg cactgggttc
    53701 acatggtcac ggcctgacca ttaaccccac aggtaatgcc gaaaacaccg tcaaaaacat
    53761 cgcatttaac tacatcgtga ggcttgcata atgaccttta aaatgagcga acacccgcag
    53821 acaattaaaa tctacaacct gtcagccgga acaaatgaat ttatcggcga aggcgatgcc
    53881 tggatccccc cgcataccgg actgcctgcg cactcaacgg acattgcccc gcctgacatt
    53941 cctgaaggac acgtggctat ctttgatgag gatgaaaacg cctggcacct catggaagat
    54001 caccggggta aaaccgtctg gcgtctggag tcggggagcg ccttactgat tactgagccg
    54061 ggcccgttac cggaaaacgt gaccatgata aaaccggacg gggagttctg ccgctgggat
    54121 ggttcatcat gggttaagga tgaagcggca gaacaggctt accgggtaag cgaggcggag
    54181 aataaaaaac ggaaattaat acaggaggca acaaatacaa taaacatcct tcaggatgcc
    54241 gtggatttaa atatggcgac cgacgatgaa agcgccctgc ttcccctgtg gaaaaaatac
    54301 cgggtattac tgagccggac agacacgggc aacgcaccgg atattgtctg gccggaatcc
    54361 ccttcgccgg atcatcacgc aggctgaccg gaggcaccat gacgtggaca agaaaaacaa
    54421 tcagcgtcag cgccgacatg cctccgctga gctgttccgt catccccgtc acaccgtgga
    54481 cttacgggct gggacgatat gaagaatcgg gggtatattt aagccctccg aacgccataa
    54541 actggctggc cgggaaaatg gccggaagcc gtgcatccgg cgacgtgacc attatcatga
    54601 ttgccgaaaa cacccacgat ctttttatac agtcaatggc ggcactgacc gccgttctgc
    54661 ccgtgccggt attcacgcag gcacagcgca tggcacaggc tgccgccagt ctgagcactg
    54721 acaaaatgca aatcccgatc accaccgaca ccctgcccgc acctgtgcat ctttccgttg
    54781 ccacaatgcg cagcgcagtc agtgccgccc gtgcagcaca ggccaggcag ctggcagcac
    54841 aacgcccgga tgccgcaggg cttcgccgcc agataagccg ctttatcagc agacgggctg
    54901 gcgcacttgc cggactggaa gacgggatca gaacactggc agaacagaag gcgcaggcct
    54961 gggtatttca gtaccggggg tatcaccgcg ccgcagcggc ggccatggtg aaggatatcc
    55021 cggcaccgac cgccgtacat actgtggccg cactcctggc ggcagactca ttaaccgaac
    55081 tggggaaaat gatccatgag ccagaccgca ctgctcgccc ttgatggtga gggtattttc
    55141 atgcagaaca tggtgatttc gccctcgatg cagttccagg aaaaggacca gtccggtcag
    55201 acatcgagca ccgcgaacgc cgagcagggc atcaaggcca aagagcttcg cgtaaccggg
    55261 atgatcacct ttgacaatga gcgggccctt cagcgcattt tccagctggc ctctgccacc
    55321 accggggacg gtgccctgaa agtttaccgt attgcaaacg ccaccgctgc cgccattaac
    55381 tttcgtgaag ggacattttc cgggcagata gacgcacagc agcagaccga ccgcctcgcc
    55441 tggcaggtca gcttcacatt acgcgaaaga cgaagcgtac cggaaaaacg ccaggcacgc
    55501 gccacccccg gcagcagccg taaacagacg ccgcagacgc ccggcgcaaa aggcgggacc
    55561 gtggccgacg aaacaccgga gaaaatgacc tggttcgaag aaaaggtcct caaacccgtc
    55621 aatgatgcgc tggagtaacc atgaacccca ctaaacgtct gttactgggt aacgacacca
    55681 ttcagctggt cagctgttcc gtcatgcttg agctgaacgc gtgcggacgc ggatttatca
    55741 cggccagaac ggagcagaac tacaccgggc gccctgttcg tctcgatatc ggatacggaa
    55801 acgacctggt gcgctggttt accggatacg ttgaacgcag ccagcccgct gaaaacggct
    55861 cggtgcggct tctgattcgt gaaatggcgg ggatccttga tcaccccttc ccctgttcat
    55921 ttcagcaccc gacaatgcgc acggtcacac agtggctgag tgagaccagc gggctggaaa
    55981 tcatcctgcc ggacaacgcg gcttacactg acagacccgt tccgcatttc acgcacagcg
    56041 gcacgggcta cgaactgctg gccagtctgg ggcgtatttt ctccgtaccg gattacatct
    56101 ggcatccgtt accggacggc ggcattatgg ccgctgccgc cgcgcagggg atgttttccg
    56161 gtcgccccgt caccatcccg cacgagttca gccaggcatc aacgggcgga cacagtatga
    56221 ccctcccgat gattcagacg ctgcgaccgg gtgtggaggt gaacggtcag cgcctgacga
    56281 tggtcatgct ggaagatgac aacatgacca tcacgtggac accgcgcaat aaagccaccg
    56341 gcgcccccct ccagaagtcc cctttccgtc gccaggctga aaaggccttc cctgaactgg
    56401 catcagggct tcacctgccc aaactggcac gtgttgaggc gccgaccgaa gccgtcagcg
    56461 cgggcaatat tgccgacccg ttcagacccc gctacgcggt gaatctccag ctgcttgacg
    56521 agaacggcaa cccggcagcg gatacgcccg tttataacgc cgttcccctc cccgttccta
    56581 tggcgggcag tgagtccggc atgttccagt tcccgccacc cggaaccctg gtggaagtgg
    56641 gatttgtgga aggccgggcg gataagccct tcatccgcca gataatggca gagggccaca
    56701 atctgcccga cgtgaagccg ggcgagcagt tgcagcaaca gcgcgacggc gtatcgcagc
    56761 gcgtgacggt cgccggagac tgggagcgcc agacagacca gcggatacag gagacatcac
    56821 gcgagcgcgt ggtgagcgca gacgaggaaa agcggagcct gaccagccgg gagaccacca
    56881 tacaggcaac cgacacaacc accgtcctgg gcgtatccac gcttcaggct ggcgcggtca
    56941 cccatatcac tgaagagaac tacagcatca gcacgagcgg gcacatgatg gtggttgccg
    57001 gtacctgtga gcgcgacgtg gaaggcgaca cccaggacac cgtgggcggc agccttaccg
    57061 aaaagattaa gggcgtccgt cacagtattg cgcaggcgca gaagctggtt ggcgatacgg
    57121 tgaagctggg aaacgagggc attaatgtac tgaccatgct gacggacctt gccgatgtgg
    57181 tggaagagct ggcagatatt acagcatcac atacccaccc aaaaacgggc acgtctccgc
    57241 aggccgcaca gttcagccag gtcgctcagg agtgccgcca gctaaaaaat aaatattcgc
    57301 caataatcga atagttattc actttaacag gccgcgcaat gtggcctttt ttattcccgg
    57361 acaataaaaa atattcatca ccggacatca ccctccacga aaaaaccgtc cgcagcagaa
    57421 aaaaagatgg acgaaacgcc ccacgtaatc cccacgtaaa acgtcgcgta acgccacgta
    57481 acgaagagat aaccccgcca gcccgattcg atcccctgac atcagaaaac gatttctgcg
    57541 ccccaaaatc gccgcaaatg gccggaaatt ttcggcggcg acgtaacgcg taaagtgacg
    57601 taatcggcgc taccccgcac acgcctccgg tatttgtgta cgataaattt tgcaaatcga
    57661 aaggggtgca aaacaccccg ccagggcgcg ccattactga ggttttctct tctgaatggt
    57721 tttgcacttt gtgcaaggtt ttgcaaaagt gtgcaaaaac tccggctttc tgaggatccc
    57781 gaaaaatttt aatgcactga ataacaagag atttttaaaa ttacgtcacg atcgaagatc
    57841 tgaaaaagga tccgacgacg aaaacgaaaa aatcaggaaa gccagaactg gcgcggcctg
    57901 gcggggagtg gcagcaaaaa tcacttttgc acacttttgc acaactgctc cgaaaaagga
    57961 tggcgaaggg gaaggaaaaa cagccacgtc atccgttgca cgtaacggac gtaaaaggta
    58021 aattacgcgc gggtgccttt cggctgacgg ctggaaggaa tacctgaagg ccggatgtgg
    58081 aaaggccccg agtcaaacat ttgtttaacc cgaggcccta acccatctac ctcgcaagtg
    58141 gaaggttagc gcctccctgt aaaaggagtc aagcgctatg ccgcaaaaaa cgatcatagt
    58201 gagcatttta tgcataacta ttttgatgat tgtctggatg atacacacgt caccgtgtga
    58261 gttcaggctt aacatcatgt ggtcacaatt tgcggcgttc ttgcagtgta aacagtaaga
    58321 aaccgcagcg ggggtgaaag cccccgctat ccggttgccg ggttcggtcg atatggcacc
    58381 cctttctcca ttggattaca gacgtatttg cccgcgacat gcgggccttt ttgttcagtt
    58441 gtatttattg tggtcgttac tggcagtggt attaactgat tttgcatgaa tagcacaaat
    58501 tttttccacg ataacaccac acgcttcaag atcttccggg cgtgaaacct ttatttttgt
    58561 aatagtctca ggattatcag ccgttccact ttgcacaacc tcaatttcaa ttttttttct
    58621 tgaaagaatc attttcatcg ccccgacaca ggacagagta agcctgtgcg aaagaatatc
    58681 accagtgcga tagcgttgtc tgcttcaacg ccaggcattg cacacacaaa gggaaatcag
    58741 tacattacac gcgggtgcct ttcggctgat ggctggaagg attacctgaa ggccggatgt
    58801 ggaaaggccc cgagtcaact ttaatgttaa cccgaggccc taacacttcc taccgtgaca
    58861 agtggaaggt tagcgcctcc ctgtaaaagg agtcaagcgc tatgccgcaa aaaacgatca
    58921 tagtgagcat tttatgcata actattttga tgattgtctg gatgatacac aagtcaccgt
    58981 gtgagttcag gcttaacatc atgtggtcag aatttgcggc gttcttgcag tgtaaacagt
    59041 aagaaaccgc agcgggggtg aaagcccccc gctatccggt tgccgggttc ggtcgatatg
    59101 gcaccttttc tccgttgaat tacatagaca tttgcccgcg atatgcgggc tatattttta
    59161 ttttttgaat aggttaataa attcaggttt tttcttatct cctgcttccc ttcgtttaac
    59221 ccaacaacac cctgactgaa gtaaacagac aaattcatcc tgcttcagct ctaaggtgtc
    59281 tttaagtttt aattgttttt tcgcagcatc aggaacaaaa acatcaacaa taatctttcc
    59341 ggggtcatac agttgagttt caatcagcac aggtaattta tggacaggtc tgggttccat
    59401 acgagaaaaa gaaaactcac catccatcac accatcaaaa gtacttacag aatcgtaacc
    59461 agaaaagcac tcatatttca cctgaagaac aacagcatca tcaagcttta aaaaatcgca
    59521 ataagaatca tacgccaggc gatcactata aaaaacatca ccacgattaa actgaaagga
    59581 atccccttgc gtccaaattt cataattaaa aaactctgtt tttactgggg taagttttat
    59641 atccaaagaa tccttgtaac actcttttac agcaaagcgc caaccagctg cgtaaccatc
    59701 aacaaatacc gcaatcaacg cattactacg gcgaaactcc cttacagtac ggatagaaag
    59761 cgagaactca tctatcatac tcttgtaagt gttaacatcc ttctcaagcg atttactgat
    59821 atcattctca accacgcagt cttgctcaag aggaatttca ccagtatcaa taaaattgat
    59881 aaattgttct tctgtcagaa taattacgcc ataatcgctg gctttctgaa ctttccgcca
    59941 ccctttgttc tttcctacac aaaggaaaaa caaacttttt ccaacatcag acttaacatc
    60001 aataccattt tcttttgcaa gagcacttaa gcgagattta tcagactggt caaatccggt
    60061 aaaacaaatt gaaattgaat t
//
LOCUS       DAZGAP010000005        55879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGAP010000005 DAZGAP010000000
VERSION     DAZGAP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.005"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..970
                     /gene="yfeH"
                     /locus_tag="ACHLAM_000253"
     CDS             <1..970
                     /gene="yfeH"
                     /locus_tag="ACHLAM_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO7851845.1"
                     /translation="TLTLITVVLLASFFPARGDFVPFFENLTTAAIALLFFMHGAKLS
                     REAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGFLYLCILPATV
                     QSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGSLEQVGKIMLQ
                     LLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVNGIWHKVGWGS
                     LLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANGIPMANILFPT
                     SVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(967..1185)
                     /gene="ypeB"
                     /locus_tag="ACHLAM_000254"
     CDS             complement(967..1185)
                     /gene="ypeB"
                     /locus_tag="ACHLAM_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO7851846.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(1187..3202)
                     /gene="ligA"
                     /locus_tag="ACHLAM_000255"
     CDS             complement(1187..3202)
                     /gene="ligA"
                     /locus_tag="ACHLAM_000255"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO7851847.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(3273..4271)
                     /gene="zipA"
                     /locus_tag="ACHLAM_000256"
     CDS             complement(3273..4271)
                     /gene="zipA"
                     /locus_tag="ACHLAM_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO7851848.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQLVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            4501..5262
                     /gene="cysZ"
                     /locus_tag="ACHLAM_000257"
     CDS             4501..5262
                     /gene="cysZ"
                     /locus_tag="ACHLAM_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO7851849.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            5447..6418
                     /gene="cysK"
                     /locus_tag="ACHLAM_000258"
     CDS             5447..6418
                     /gene="cysK"
                     /locus_tag="ACHLAM_000258"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO7851850.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            6802..7059
                     /gene="ptsH"
                     /locus_tag="ACHLAM_000259"
     CDS             6802..7059
                     /gene="ptsH"
                     /locus_tag="ACHLAM_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO7851851.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            7104..8831
                     /gene="ptsI"
                     /locus_tag="ACHLAM_000260"
     CDS             7104..8831
                     /gene="ptsI"
                     /locus_tag="ACHLAM_000260"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO7851852.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     TDLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            8872..9381
                     /gene="crr"
                     /locus_tag="ACHLAM_000261"
     CDS             8872..9381
                     /gene="crr"
                     /locus_tag="ACHLAM_000261"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO7851853.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(9423..10274)
                     /gene="pdxK"
                     /locus_tag="ACHLAM_000262"
                     /gene_synonym="thiJ"
     CDS             complement(9423..10274)
                     /gene="pdxK"
                     /locus_tag="ACHLAM_000262"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO7851854.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            10379..10747
                     /gene="yfeK"
                     /locus_tag="ACHLAM_000263"
     CDS             10379..10747
                     /gene="yfeK"
                     /locus_tag="ACHLAM_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO7851855.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDDHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(10750..11661)
                     /gene="cysM"
                     /locus_tag="ACHLAM_000264"
     CDS             complement(10750..11661)
                     /gene="cysM"
                     /locus_tag="ACHLAM_000264"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO7851856.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(11795..12892)
                     /gene="cysA"
                     /locus_tag="ACHLAM_000265"
     CDS             complement(11795..12892)
                     /gene="cysA"
                     /locus_tag="ACHLAM_000265"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO7851857.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(12882..13757)
                     /gene="cysW"
                     /locus_tag="ACHLAM_000266"
     CDS             complement(12882..13757)
                     /gene="cysW"
                     /locus_tag="ACHLAM_000266"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO7851858.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(13757..14590)
                     /gene="cysT"
                     /locus_tag="ACHLAM_000267"
     CDS             complement(13757..14590)
                     /gene="cysT"
                     /locus_tag="ACHLAM_000267"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO7851859.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(14590..15606)
                     /gene="cysP"
                     /locus_tag="ACHLAM_000268"
     CDS             complement(14590..15606)
                     /gene="cysP"
                     /locus_tag="ACHLAM_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO7851860.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(15764..16555)
                     /gene="ucpA"
                     /locus_tag="ACHLAM_000269"
     CDS             complement(15764..16555)
                     /gene="ucpA"
                     /locus_tag="ACHLAM_000269"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO7851861.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(16684..17541)
                     /gene="murR"
                     /locus_tag="ACHLAM_000270"
     CDS             complement(16684..17541)
                     /gene="murR"
                     /locus_tag="ACHLAM_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO7851862.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSELVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            17705..18601
                     /gene="murQ"
                     /locus_tag="ACHLAM_000271"
     CDS             17705..18601
                     /gene="murQ"
                     /locus_tag="ACHLAM_000271"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO7851863.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSQEGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGPEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            18605..20029
                     /gene="murP"
                     /locus_tag="ACHLAM_000272"
     CDS             18605..20029
                     /gene="murP"
                     /locus_tag="ACHLAM_000272"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO7851864.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            20034..21335
                     /gene="pbp4b"
                     /locus_tag="ACHLAM_000273"
     CDS             20034..21335
                     /gene="pbp4b"
                     /locus_tag="ACHLAM_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO7851865.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVADALYSQDKGQTLEMIKRTPLEYQPCSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSDTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(21393..22292)
                     /gene="yfeX"
                     /locus_tag="ACHLAM_000274"
     CDS             complement(21393..22292)
                     /gene="yfeX"
                     /locus_tag="ACHLAM_000274"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO7851866.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSIHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22388..22963)
                     /gene="yfeY"
                     /locus_tag="ACHLAM_000275"
     CDS             complement(22388..22963)
                     /gene="yfeY"
                     /locus_tag="ACHLAM_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO7851867.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(23024..23473)
                     /gene="yfeZ"
                     /locus_tag="ACHLAM_000276"
     CDS             complement(23024..23473)
                     /gene="yfeZ"
                     /locus_tag="ACHLAM_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HFO7851868.1"
                     /translation="MKSTEFHPVHYDAYGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23460..23885)
                     /gene="ypeA"
                     /locus_tag="ACHLAM_000277"
     CDS             complement(23460..23885)
                     /gene="ypeA"
                     /locus_tag="ACHLAM_000277"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO7851869.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            24099..24968
                     /gene="amiA"
                     /locus_tag="ACHLAM_000278"
     CDS             24099..24968
                     /gene="amiA"
                     /locus_tag="ACHLAM_000278"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO7851870.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            25038..25866
                     /gene="hemF"
                     /locus_tag="ACHLAM_000279"
                     /pseudo
     CDS             25038..25866
                     /gene="hemF"
                     /locus_tag="ACHLAM_000279"
                     /inference="COORDINATES: protein motif:HMM:NF013390.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
     gene            complement(25800..26924)
                     /gene="eutR"
                     /locus_tag="ACHLAM_000280"
     CDS             complement(25800..26924)
                     /gene="eutR"
                     /locus_tag="ACHLAM_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO7851871.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSSG"
     gene            complement(26970..27470)
                     /gene="eutK"
                     /locus_tag="ACHLAM_000281"
     CDS             complement(26970..27470)
                     /gene="eutK"
                     /locus_tag="ACHLAM_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO7851872.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27483..28142)
                     /gene="eutL"
                     /locus_tag="ACHLAM_000282"
     CDS             complement(27483..28142)
                     /gene="eutL"
                     /locus_tag="ACHLAM_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO7851873.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28152..29039)
                     /gene="eutC"
                     /locus_tag="ACHLAM_000283"
     CDS             complement(28152..29039)
                     /gene="eutC"
                     /locus_tag="ACHLAM_000283"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO7851874.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(29060..30421)
                     /gene="eutB"
                     /locus_tag="ACHLAM_000284"
     CDS             complement(29060..30421)
                     /gene="eutB"
                     /locus_tag="ACHLAM_000284"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO7851875.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30433..31836)
                     /gene="eutA"
                     /locus_tag="ACHLAM_000285"
     CDS             complement(30433..31836)
                     /gene="eutA"
                     /locus_tag="ACHLAM_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO7851876.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTEARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31833..33059)
                     /gene="eutH"
                     /locus_tag="ACHLAM_000286"
     CDS             complement(31833..33059)
                     /gene="eutH"
                     /locus_tag="ACHLAM_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO7851877.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(33276..34463)
                     /gene="eutG"
                     /locus_tag="ACHLAM_000287"
     CDS             complement(33276..34463)
                     /gene="eutG"
                     /locus_tag="ACHLAM_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO7851878.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSLVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(34453..35289)
                     /gene="eutJ"
                     /locus_tag="ACHLAM_000288"
     CDS             complement(34453..35289)
                     /gene="eutJ"
                     /locus_tag="ACHLAM_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO7851879.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKTEGIY
                     AK"
     gene            complement(35300..36703)
                     /gene="eutE"
                     /locus_tag="ACHLAM_000289"
     CDS             complement(35300..36703)
                     /gene="eutE"
                     /locus_tag="ACHLAM_000289"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO7851880.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(36715..37002)
                     /gene="eutN"
                     /locus_tag="ACHLAM_000290"
     CDS             complement(36715..37002)
                     /gene="eutN"
                     /locus_tag="ACHLAM_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO7851881.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(37109..37402)
                     /gene="eutM"
                     /locus_tag="ACHLAM_000291"
     CDS             complement(37109..37402)
                     /gene="eutM"
                     /locus_tag="ACHLAM_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO7851882.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(37441..38457)
                     /gene="pta"
                     /locus_tag="ACHLAM_000292"
     CDS             complement(37441..38457)
                     /gene="pta"
                     /locus_tag="ACHLAM_000292"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7851883.1"
                     /translation="MIIERCRKLALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALDKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVQRQTEVNRESSLQ
                     TLVE"
     gene            complement(38454..39257)
                     /gene="eutT"
                     /locus_tag="ACHLAM_000293"
     CDS             complement(38454..39257)
                     /gene="eutT"
                     /locus_tag="ACHLAM_000293"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO7851884.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQSLTVKQIQQRLGETQ"
     gene            complement(39254..39955)
                     /gene="eutQ"
                     /locus_tag="ACHLAM_000294"
     CDS             complement(39254..39955)
                     /gene="eutQ"
                     /locus_tag="ACHLAM_000294"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO7851885.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(39930..40409)
                     /gene="eutP"
                     /locus_tag="ACHLAM_000295"
     CDS             complement(39930..40409)
                     /gene="eutP"
                     /locus_tag="ACHLAM_000295"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO7851886.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40422..40757)
                     /gene="eutS"
                     /locus_tag="ACHLAM_000296"
     CDS             complement(40422..40757)
                     /gene="eutS"
                     /locus_tag="ACHLAM_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO7851887.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(41050..43329)
                     /gene="maeB"
                     /locus_tag="ACHLAM_000297"
     CDS             complement(41050..43329)
                     /gene="maeB"
                     /locus_tag="ACHLAM_000297"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO7851888.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYRGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            43618..44568
                     /gene="tal"
                     /locus_tag="ACHLAM_000298"
     CDS             43618..44568
                     /gene="tal"
                     /locus_tag="ACHLAM_000298"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7851889.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            44588..46591
                     /gene="tkt"
                     /locus_tag="ACHLAM_000299"
     CDS             44588..46591
                     /gene="tkt"
                     /locus_tag="ACHLAM_000299"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7851890.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(46694..47737)
                     /gene="ypfG"
                     /locus_tag="ACHLAM_000300"
     CDS             complement(46694..47737)
                     /gene="ypfG"
                     /locus_tag="ACHLAM_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO7851891.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(47863..48438)
                     /gene="nudK"
                     /locus_tag="ACHLAM_000301"
     CDS             complement(47863..48438)
                     /gene="nudK"
                     /locus_tag="ACHLAM_000301"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO7851892.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFEQYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(48506..50485)
                     /gene="aegA"
                     /locus_tag="ACHLAM_000302"
     CDS             complement(48506..50485)
                     /gene="aegA"
                     /locus_tag="ACHLAM_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO7851893.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QVQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            50691..52391
                     /gene="narQ"
                     /locus_tag="ACHLAM_000303"
     CDS             50691..52391
                     /gene="narQ"
                     /locus_tag="ACHLAM_000303"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO7851894.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            52555..55668
                     /gene="acrD"
                     /locus_tag="ACHLAM_000304"
     CDS             52555..55668
                     /gene="acrD"
                     /locus_tag="ACHLAM_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO7851895.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAQLVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(55767..55826)
                     /gene="ypfM"
                     /locus_tag="ACHLAM_000305"
     CDS             complement(55767..55826)
                     /gene="ypfM"
                     /locus_tag="ACHLAM_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO7851896.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        12909 a        15488 c        14420 g        13062 t
ORIGIN      
        1 caccttaacc ctgatcacgg tggtgttgct ggcctctttc tttccggcca gaggcgattt
       61 cgtccccttc tttgaaaatc tgaccactgc agctattgcc ctgctgttct ttatgcacgg
      121 cgcgaagttg tcgcgtgagg cgattattgc tggcggtggg cactggcgac tgcatttgtg
      181 ggtaatgtgc agcaccttcg tattgtttcc gattctgggc gtgctgtttg cctggtggaa
      241 accggtaaat gtcgacccga tgctctactc cggttttctc tacttgtgca ttctccccgc
      301 taccgtgcag tctgcaatcg ccttcacgtc aatggcgggc ggtaacgttg cggcggcggt
      361 ttgttctgcg tcagcatcca gcctgcttgg gattttcctt tcaccattgc tggttggtct
      421 ggtgatgaat gttcacggtg cgggaggcag ccttgagcag gtcggtaaaa ttatgctgca
      481 attgctactg ccgtttgtgt tggggcatct ttcccggccg tggattggtg actgggtgtc
      541 gcgcaataaa aaatggattg cgaaaactga ccagacgtcc attctgttgg tggtttatac
      601 ggcattcagc gaagccgtcg ttaacggtat ctggcacaaa gttggctggg gatcattgct
      661 gtttatcgtg gtggtcagtt gcgttcttct ggctatcgtg attgtagtta acgtctttat
      721 ggcacgccga ctgggcttca ataaggcaga tgaaattact atcgtctttt gtggttcgaa
      781 aaagagtctg gcaaatggca tcccgatggc aaacattctg ttccccacat cggtgatcgg
      841 tatgatggtg ctgcccctga tgattttcca tcagatccaa ttgatggtct gtgcggtgct
      901 ggcgcgtcga tacaaacgcc agaccgaaca gttacaggcg cagcaggaaa gcagcgccga
      961 taaagcttaa agcggacgct tcaggggctg gaccagttgc gtcagcccct cggttttaat
     1021 cagcagcgtg atttgcatta gctcaccgag cttccctgcc gggaactcat ctttgcgggc
     1081 aaaccacagc agatactcct ccggcaggtc gattaagcga cgccctttgt acttgccaaa
     1141 cggcattatc gtattggcta tttcaatcag ctgctctttt tccatctcag ctacccagca
     1201 aacgcagcat ttccgtttcg tcgatgactt caatacccag ttcctgcgcc ttcgccagtt
     1261 tagatcctgc agcttcaccc gctatcacca gatcggtttt cttcgacacg ctgcccgcga
     1321 ctttcgcgcc cagttcgacc agtcgagctt tagcgtcatc acgcgacatc tggcttaagc
     1381 tgcccgtaag caccacggtt ttaccagcaa acgggctgtc aatctcttcc gcgttaatca
     1441 ccaccggcgc aggccagtga acgccttccg ccaacagctc gctgatgaca ttgcggttgc
     1501 tttcttcggc aaagaagttg tgaacgtggg aggcaacgac aatgccaaca tcaggcacct
     1561 tttgcagctc ttcaatcgaa gcggcttcca gcgcttccag cgtgccgaaa tatgccgcca
     1621 gacctgctgc ggtggcctcg ccgacttcac ggatgccaag tgcatagagg aagcgagcaa
     1681 aggtggtttc tttcgctttt tccagcgcgt taaccacgtt ttgtgccgat ttcggcccca
     1741 tacgctccag tccggtcagt ttgactgcgg tgagtttgaa cagatccgcc ggagtgtgga
     1801 catattcttt ttcaaccagt tgatcgatga ttttgtcgcc cattccgtca acatccatcg
     1861 cacggcggga aacaaagtgt ttcagagact ctttacgctg cgcaccgcaa atcaggccac
     1921 cggtacagcg ggcaaccgct tcaccttcca cacgctcgac gtcagaacca cataccggac
     1981 aatgcgtcgg gaatacaacc tcacgggtat cttccgggcg ttcagaaagc acgacgttaa
     2041 ccacctgcgg gatcacgtcg ccagcgcggc gaataaccac tttatcgcca atgcgtaaac
     2101 caagacgttc gatttcgtca gcattatgca gtgtagcgtt actcaccaac acgccagcaa
     2161 catggacagg ttccagacgc gcaacaggcg taatcgcgcc agtacgccca acctgaaact
     2221 cgacgtcacg cacaaaggtc atctgttcct gcgccgggaa tttaaacgct accgcccagc
     2281 gcggggcacg cgcgacaaag ccaagctgct cctgctgtgc cagtgagttg accttaatca
     2341 ccacgccgtc gatatcaaag cccagcgtcg gacggtcttt ttctacttcg tgataaaacg
     2401 ccagcacttc ttccgccgat tcacaaagtg ttacccgatc actgacaggt aatccccacg
     2461 ctttaaattg cattaagcgt cccaaatggg tatccggcaa ttcaccgcct tccagaacac
     2521 caacgccata gcagaagaaa gtgagcggcc gcttcgctgt aatacgcgga tcaagctgac
     2581 gcagtgaacc agctgccgca ttacgtgggt tagcaaacac tttcccgccc gtgcgtcgcg
     2641 catcttcgtt aatcttttcg aaccccgcct gcggcaggaa cacttcacca cgcacttcca
     2701 gacgcgccgg gatattctct ccgtgtagct tcagcggaat ggcgcgaata gtacgcacat
     2761 tagacgtgat atcttccccg gtggtgccat cgccacgggt ggcggcactg actaaaacac
     2821 cattttcata cagaatactg acggcaagac catccagctt cagctcacag caccaggtga
     2881 ctttctcgtt gcttttcaga cggtcctgca cacgtttgtt gaaagcaaga aagctttctt
     2941 catcaaaaac gttatccagc gacagcattg gcacttcatg gcggatctgg ctgaaagccg
     3001 ccagcggcgc agcacctaca cgttgggtag gcgaatcagg cgtaatcagt tctggatgtt
     3061 tggtttccag ctcgcgcaat tcgcgcatca gcctgtcgta ttcagcgtcg ggaatttccg
     3121 gcgcatccat cacatgataa agatattcat gatggcgaag cgtcgttcgc agttctgtca
     3181 gttgttgttc gattgattcc atatcgcacc atcaatgcta aaaacccccg acaagcgggg
     3241 gttcgaagag gagttaattt gccttaagtg tatcaggcgt tggcgtcttt gacttcgcgg
     3301 atgatgtcct ggtactcgcg caatttctgc ggagtcatca tacggcgctg atcgtcaaga
     3361 acaacaccgc ccacttcatc ggcaatatgc tgcgcagatt gcagcatcag cttgaagttc
     3421 tgcagctcgt caccgtaaga cggtacctgc ataaagatag tgacacccgg agtagtgaaa
     3481 tccttcattt caggatcaaa ggttcccggt ttcaccatat tcgccaggct gaataacgcc
     3541 gggccgctgc catccgggct aagatgacga tggtaaatat tcatatcgcc aaaaatgaag
     3601 cccgcttgtt gaatgctgtt aagaagcagt tcaccgttta gctcgctacc gtgatgcgcc
     3661 gcgacgttca tgataatcac cgcttctttg cgcttcggtt tatccataac tggagccggt
     3721 tccgctacag gctcaggctg tggtgccgct acgggttctg caggctggaa agcctgttgt
     3781 gccggttgcg gtgctgaatg cactggctgc ggcgctggcg cgacctgtgg cgaaactggc
     3841 tgctgcaacg gctgttcagg ctgcggctga taggcaggct gctgcacggg ttgctgcacc
     3901 agctgcgctg gacgcggagc atgttgcggc ggtacctgcg cttcaggcgg ctgctggacc
     3961 ggttgacgcg gctgcgcaga cgcataaggc ggttggtact ggtgttgcgg cgacggacga
     4021 gcagcctcat gctcctgggc gttagccggg gcatgattca cgcggtgaac tcgaacctca
     4081 ccaacgccct catcatcttc gacatcctcg tcataagaat cgtcgtcacg ttttgacttc
     4141 attcgtttta atggccgatc gcggaacata gaagatcgtt ctttacggct ggtccagaaa
     4201 ccatgtacca gtaaagcgat tatggcgatc gcgccaacaa tgattaatat cagacgcaaa
     4261 tcctgcatca ttatattctc tgttgttcta acaccttgcc accacggcaa acatttactc
     4321 actaagagta tttgtcgatt acctcaagtg caagtgcact attaactttc acagcacaaa
     4381 gatagatgaa atcgtgcttt ttgctgtttt ttcgaacata tcctaactgt ccattgcgca
     4441 attacccggt aaaatacgca gaattttcct gggattggtc aaaaggagct catcctgatt
     4501 atggtttcat cattcacatc tgccccacgc agcggttttt actattttgc gcaaggctgg
     4561 aagctcgtct cgcaacctgg gattcggcgt ttcgttattt taccgctgct ggtcaatatt
     4621 ttgttgatgg ggggcgcatt ctggtggctc tttacgcaac tcgatgtctg gatcccgact
     4681 ctcatgagtt atgttccgga ctggttacaa tggctgagct atctgttgtg gcccctggct
     4741 gtcatctctg tgctgttagt atttggttat ttcttttcaa cgattgctaa ctggattgcc
     4801 gctccgttca acggtttatt ggctgaacaa ctggaagcaa gactgactgg cgctacaccg
     4861 ccagataccg gaattttcgg tatcatgaaa gatgtgccgc gaatcatgaa acgcgaatgg
     4921 caaaaatttg cctggtatct gccgcgcgca attgtattgc taattcttta cttcatccct
     4981 ggtattgggc aaaccgtcgc gccagtactg tggttcctgt ttagcgcctg gatgttagcc
     5041 atccagtatt gcgattaccc cttcgataac cacaaagtgc cgtttaaaga gatgcgcacc
     5101 gccctgcgca cacgcaaaat caccaatatg cagtttggtg ctttaaccag cctgtttacg
     5161 atgatcccgt tgcttaatct gttcatcatg cccgttgccg tttgtggcgc gacggcgatg
     5221 tgggtcgatt gctatcgcga taaacacgcg atgtggcggt aacaatctac cggttatttt
     5281 gtaaaccgtt tgtgtgaaac aggggtggct tatgccgccc cttattccat cttgcatgtc
     5341 attatttccc ttctgcatat agatatgcta aatccttact tccgcatatt ctctgagcgg
     5401 gtatgctacc tgttgtatcc caatttcata cagttaagga caggccatga gtaagatttt
     5461 tgaagataac tcgctgacta tcggtcacac gccgctggtt cgcctgaatc gcatcggtaa
     5521 cggacgcatt ctggcgaagg tggaatctcg taaccccagc ttcagcgtta agtgccgtat
     5581 cggtgccaac atgatttggg atgccgaaaa gcgcggcgtg ctgaaaccag gcgttgaact
     5641 ggttgaacca accagcggta ataccgggat tgcactggcc tatgtcgctg ccgctcgcgg
     5701 ttacaaactc accctgacta tgccagaaac catgagtatt gaacgccgca agctgctgaa
     5761 agcgttaggt gcaaacctgg tgctgaccga aggtgctaaa ggcatgaaag gcgcaatcca
     5821 aaaagcagaa gaaattgtcg ccagcaatcc agagaaatac ctgctgctgc aacaattcag
     5881 caatccggca aaccctgaaa ttcacgaaaa aaccaccggt ccggagatct gggaagatac
     5941 cgacggtcag gttgatgtat ttattgctgg cgttgggact ggcggtacgc tgactggcgt
     6001 cagccgctac attaaaggca ccaaaggcaa gaccgatctt atctctgtcg ccgttgagcc
     6061 aaccgattct ccagttatcg cccaggcgct ggcaggtgaa gagattaaac ctggtccgca
     6121 taaaattcag ggtattggcg ctggttttat cccggctaac cttgatctca agctggtcga
     6181 caaagtcatt ggcatcacca atgaagaagc catttctacc gcacgtcgtc tgatggaaga
     6241 agaaggcatt cttgctggta tctcttctgg agcagctgtt gctgcggcgt tgaaactaca
     6301 agaagatgaa agctttacca ataagaatat tgtggttatt ctgccgtcat cgggtgagcg
     6361 ttatttaagc actgcattgt ttgccgatct tttcactgag aaagaactgc aacagtaatg
     6421 ccagcttgta aaaaatgcgt aaaaaagcac ctttttaggt gcttttttgt ggcctgcttc
     6481 aaactttcgc ccctcctggc attgattcag cctgtcggaa ctggtattta accagactaa
     6541 ttattttgat gcgcgaaatt aatcgttaca ggaaaagcca aagctgaatc gattttatga
     6601 tttggttcaa ttcttccttt agcggcataa tgtttaatga cgtacgaaac gtcagcggtc
     6661 aacacccgcc agcaatggac tgtattacgc tcttcgtgcg tcgcgtctgt taaaaactgg
     6721 cgctaacaat acaggctaaa gtcgaaccgc caggctagac tttagttcca caacactaaa
     6781 cctataagtt ggggaaatac aatgttccag caagaagtta ccattaccgc tccgaacggt
     6841 ctgcacaccc gccctgctgc ccagtttgta aaagaagcta agggcttcac ttctgaaatt
     6901 actgtgactt ccaacggcaa aagcgccagc gcgaaaagcc tgtttaaact gcagactctg
     6961 ggcctgactc aaggtaccgt tgtgactatc tccgcagaag gcgaagacga gcagaaagcg
     7021 gttgaacatc tggttaaact gatggcggaa ctcgagtaat ttcccgggtt cttttaaaaa
     7081 tcagtcacaa gtaaggtagg gttatgattt caggcatttt agcatccccg ggtatcgctt
     7141 tcggtaaagc tctgcttctg aaagaagacg aaattgtcat tgaccggaaa aaaatttctg
     7201 ccgaccaggt tgatcaggaa gttgaacgtt ttctgagcgg tcgtgccaag gcatcagccc
     7261 agctggaaac gatcaaaacg aaagctggtg aaacgttcgg tgaagaaaaa gaagccatct
     7321 ttgaagggca tattatgctg ctcgaagatg aggagctgga gcaggaaatc atagccctga
     7381 ttaaagataa gcacatgaca gctgacgcag ctgctcatga agttatcgaa ggtcaggctt
     7441 ctgccctgga agagctggat gatgaatacc tgaaagaacg tgcggctgac gtacgtgata
     7501 tcggtaagcg cctgctgcgc aacatcctgg gcctgaagat tatcgacctg agcgccattc
     7561 aggatgaagt cattctggtt gccactgatc tgacgccgtc cgaaaccgca cagctgaacc
     7621 tgaagaaggt gctgggtttc atcaccgacg cgggtggccg tacttcccac acctctatca
     7681 tggcgcgttc tctggaactg cctgctatcg tgggtaccgg tagcgtcacc tctcaggtga
     7741 aaaatgacga ctatctgatt ctggatgccg taaataatca ggtttacgtc aatccaacca
     7801 acgaagttat tgataaaatg cgcgctgttc aggagcaagt ggcttctgaa aaagcagagc
     7861 ttgctaaact gaaagatctg ccagctatta cgctggacgg tcaccaggta gaagtgtgcg
     7921 ctaacattgg tacggttcgt gacgttgaag gtgcagagcg taacggcgct gaaggcgttg
     7981 gtctgtatcg tactgagttc ctgttcatgg accgcgacgc gctgcccact gaagaagaac
     8041 agtttgctgc ttacaaagca gtggctgaag cgtgtggctc gcaggcggtt atcgttcgta
     8101 ccatggacat cggcggcgac aaagagctgc catacatgaa cttcccgaaa gaagagaacc
     8161 cgttcctcgg ctggcgcgct atccgtatcg cgatggaccg taaagagatc ctgcgcgatc
     8221 agctccgcgc tatcctgcgt gcctcggctt tcggtaaatt gcgcattatg ttcccgatga
     8281 tcatctctgt tgaagaagta cgtgcactgc gcaaagagat cgaaatctac aaacaggaac
     8341 tgcgcgacga aggtaaagcg tttgacgagt caattgaaat cggcgtaatg gtggaaacac
     8401 cggctgccgc aacaattgca cgtcatttag ccaaagaagt tgatttcttt agtatcggca
     8461 ccaatgattt aacgcagtac actctggcag ttgaccgtgg taatgatatg atttcacacc
     8521 tttaccagcc aatgtcaccg tccgtgctga acttgatcaa gcaagttatt gatgcttctc
     8581 atgctgaagg caaatggact ggcatgtgtg gtgagcttgc aggcgatgaa cgtgctacac
     8641 ttctgttgct ggggatgggt ctggacgaat tctctatgag cgccatttct atcccgcgca
     8701 ttaagaagat tatccgtaac acgaacttcg aagatgcgaa ggtgttagca gagcaggctc
     8761 ttgctcaacc gacaacggac gagttaatga cgctggttaa caagttcatt gaagaaaaaa
     8821 caatctgcta atccacgaga tgcggcccaa tttactgctt aggagaagat catgggtttg
     8881 ttcgataaac tgaaatctct ggtttccgac gacaagaagg ataccggaac tattgagatc
     8941 attgctccgc tctctggcga gatcgtcaat atcgaagacg tgccggatgt cgtttttgcg
     9001 gaaaaaatcg ttggtgatgg cattgctatc aaaccaactg gaaacaaaat ggtcgcgcct
     9061 gtagacggca ccattggtaa aatctttgaa accaaccacg cgttctctat cgaatctgat
     9121 agcggcgttg aactgttcgt ccacttcggt atcgacaccg ttgaactgaa aggcgaaggc
     9181 ttcaagcgta ttgctgaaga aggtcagcgc gtgaaagttg gcgatactgt cattgaattt
     9241 gatctgccgc tgctggaaga gaaagccaag tctaccctga ctccggttgt tatctccaac
     9301 atggacgaaa tcaaagaact gatcaaactg tccggtagcg tgactgtggg tgaaaccccg
     9361 gttatccgca tcaagaagta attcttgccg cagtgaaaaa tggcgcccat cggcgccatt
     9421 ttttatgctt ccgccagcgg cggcaaaatc aattcatcgc tctcatgctg ctgggtgtag
     9481 cgcatcactt ccagtacgcg caaccccgca cggtgcaccg catcgtttaa cgccttccct
     9541 ttcagcaatc cactgataag ctgagcacaa aacaggtcgc cagtcccttt caggtcggtt
     9601 tttacccgtg aatgggaaat gacattcacg ctgtcggcac tgaccaccac cacctgcatc
     9661 tcctgatttt cttcattacc ggaggcgctg gtaatcacca cccattttaa tgtgtctgaa
     9721 agcagacttt ttgcggcagc aatggcactg tcgagatcgc ggcaattttt accggtcagg
     9781 atttccagct caaagatatt gggggtaatt ccctgcgcca gcggcagtaa atattgtcga
     9841 tacgcctcgg gaaggtcagg tttgacataa attccgctat caatatcgcc aatcaccgga
     9901 tcgaccatga tcaataggtc aggatggtct ttgcgtagcg cagtcagcca ctcggcaagg
     9961 attttgattt gcgatgccgt tcccatatag cccgtggtta cagctcgaag ttggcgcagc
    10021 gcatcacgct cctgaagcgc acgcaaatag ccgctaaacc attcatccgg aatcgcacca
    10081 ccgtagaaag tgtcataatg cggtgtattg ctcagcaata ccgtcggcac ggcaaagaca
    10141 ttcaggccgt tctgtttgat agcaggcacc gcaatgctgt tgcctacgct gccgtacact
    10201 acctgcgact gcacggcgac gatatccgcc tgcaatgccc tgctcttatc gttaaacaac
    10261 aacaaactac tcatttaatt ttttctcctt gccgatgatc ctcatcgtat tccaaccgaa
    10321 actttacctg attctggcag tcaaatcggc tatcacaaaa caaggataag gtaattcaat
    10381 gaagaaaatc atttgtctgg tcattacgct gctaatgaca ctccccgttt acgcgaagtt
    10441 aactgcccat gaagaagccc gtatcaacgc catgctggag ggattagcac agaaaaagga
    10501 tttgatattt gtgcgtaacg gcgatgatca tacctgcgat gaagcggttt cccacctgcg
    10561 tctgaagctc ggcaataccc gtaaccgcat tgacactgcc gagcagttta ttgataaggt
    10621 agcctcgtcg tcatcgatta ccgggaagcc gtatatcgtg aagatgcccg gtaaaagcga
    10681 tgagaacgca caaccttttt tacatgcgct gattgcgcag acggataaaa cggtgcctgc
    10741 gcaataatct taaatccccg ccccctggct aaaatgctct tccccaaaca ccccggtaga
    10801 aaggtagcga tcgccgcgat cgcagatgat cgccacaacc accgcgccgg ggttagcttt
    10861 tgccacgcgc agtgctccgg caaccgcgcc gccggagctg acgccacaga atattccttc
    10921 ccgcactgcc agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat ccagcacctc
    10981 atccaccagc gaagcgttga aaatccccgg cagatattcc gcaggccagc ggcgaatgcc
    11041 gggaatgcta ctgccctctt ccggctgcag gccaacaatg gtcaccggtt tggattgttc
    11101 gcgcataaag cgtgaaacgc cggtgatagt gccggttgtt cccatgctgg agacaaaatg
    11161 ggtaatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt aatgggcgaa
    11221 gggattatca ggattattga actgatcgag cagctttcct tcaccacgat tggccatctc
    11281 cagcgccaga tcgcgcgcgc cttccatgcc ctgctctttg gtgacaagaa tcagttccgc
    11341 accgtatgcc agcatcgctg cacggcgctc ctggctcatg ttgtcgggca tcagcaattt
    11401 catgcgatag cctttaagcg cggcaatcat tgccagcgca atgccggtgt taccactggt
    11461 ggcttcaatc aggacatcgc ccggtttgat ttctccgcgc ttttccgcct cgacgatcat
    11521 cgaaagcgct gcacgatctt tcaccgaacc tgccgggtta ttgccttcca gtttcaacca
    11581 gacttcactg ccgttatccg gcgccattcg ctgcaacttc accagaggcg tattgccaat
    11641 tgtttgttct aatgtactca cgatctctgt ccatacgtgg tgttgcctga tgcgacgctt
    11701 gcgcgtctta tcaggcctac aggttacaaa ccttgccatt aaaaagcccg gatcgcggga
    11761 agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg agagcaagtt
    11821 cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt tgcagaccaa
    11881 cgaataaacg ctcgccacgc tgcggggcat cgtcgccatg catcacaacc gtcagcggtt
    11941 cgttgtacca ccccagcggc tgcacgacta attgggtgta gtggcctttc gggctggctt
    12001 ccagtacctg taccggcagc ggcgaatcga ggctggtacg gcggctgata tccacttccc
    12061 acggacgcag gaagagatcc accggcccct gatacgcagg tgtatagccc agcggccagc
    12121 gatgtgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg ttcacttcgc
    12181 ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctggtcc ggtgcatcag
    12241 cctgctcgat attgccctgg ctcatcacca ctacacgatc ggctacttcg gtcgcttctt
    12301 cctggtcatg ggtcacaaac acgctggtga atttcagctc ttcatgtaac tgacgcagcc
    12361 agcgacgcaa ctctttacgc acctgcgcat ccagcgcgcc aaacggttca tcaagcagca
    12421 gaatttgcgg ttctacagcc agcgcgcgcg ccagtgccac acgctgtttc tgaccgccgg
    12481 aaagctgtgc tggataacga tccgccagat gcgcaagctg caccatctcc agcaattttg
    12541 tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc accgtcaggc
    12601 cgaaagcgat attgtcgaac accgtcatat ggcggaacag cgcatagtgc tggaacacga
    12661 aaccgacttt acgatcgcgc gcatgcaggc ggctgacgtc ggtgccgtgg aagcgaatat
    12721 gcccgctggt ttgatgctcc agcccggcga taatgcgcag cagcgtggtt ttcccggaac
    12781 cggacggccc cagcaacgcg accatctgac cagaaggaat atccagtgag atatcgttca
    12841 gcacctgggt gcgaccaaaa gacttcttaa tattggcaat ctcaatgctc atgatgttcc
    12901 tcctgctgcg cgcgtttttc ctgattctcc agacgccact gcaacatact ctttaaaaac
    12961 agggtgataa tcgccatcag cgtcaacaac gcggcagcgg taaaggagcc gacggtgttg
    13021 tagtcttgct ccagcaattc aatctgtaac ggcagcgaaa gtgtttcgcc gcgaatcgag
    13081 ccggaaacca ccgacaccgc accaaactcg ccaatcgcgc gggcgttggt cagcaccacg
    13141 ccataaagca gcgcccagcg gatgttcggc agcgtgacgc ggcgaaacat ctgccagcca
    13201 gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa catcaccggc
    13261 accagctcac gcaccacaaa cggacacgtc acgaagatgg tgaccagcac cattcccggc
    13321 caggagaaca taatttgcag attatgttca tccagccagc cgcccagcgg gccgttagag
    13381 ccgtagaaca gcagatacac cagaccagca accaccggcg acacggcaaa cggaatgtcc
    13441 aacagcgtca gcaatagctg gcgtccgggg aagttaaagc gcgtcaccag ccaggccagc
    13501 agaataccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac cgtcagccag
    13561 atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag ccctttgctg
    13621 aacgcctgta cgaagatata aatcatcggc accagcagga tgaacgccga taccagcatc
    13681 ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata acgcttcaac
    13741 tgtgtcactt ccgccattaa tgacctacca cacgccgacc aaagcgactt tgcagagtgt
    13801 taattgagaa cagcagcagc agcgatgccg cgaggatcac cgaggcaatc gcgctcgccg
    13861 ccgggtaatc aaactcctgt aagcgaacaa agatcatcag cgacgtcact tctgttttcc
    13921 aggcgatatt cccggcgata aaaatcaccg cgccaaactc accgaggctg cgggtaaacg
    13981 acagcgccac acccgccacc agcgccggag atagttccgg cagcaccact ttgcgaaaac
    14041 tctgccagcg tgttgcgccg agcgtttctg ccgcctcttc atattccggg cctaactctt
    14101 ccagcaccgg ctgcacggtg cgcaccacaa acggaatgct ggtaaaggcc atcgccaccg
    14161 caatccccag ccaggtatag gtgactttga tatcaaactt cgccagccat tcaccgtaaa
    14221 aaccgttcac ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt ggcagcgcaa
    14281 agggtaaatc catcagcgca tcaagcagcg tgcgacccgg aaagcgatag cgggttaaga
    14341 tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac gccgacagca
    14401 gcgtcacttt ataggctgcg accacctgcg ggttggtgat cacctcccag tactgcgccc
    14461 agctcatctg ggccagttgc atcaccagcg cggagagcgg cagcagcaaa atcaggcaca
    14521 caaacagcag actggtgccg aggcttaagg taaagcccgg cagcacgcgt cttgaagaaa
    14581 ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct aactcgccgc cgctggtgaa
    14641 gtgggttttc atcacttccg gccaggagcc aaatttatct tccacgcgga acagctcggt
    14701 ctgcgggaat ttatctttca gtttgcccat gacttccggg ttatttacgc ggtagtaata
    14761 gtcggtgatg atggtttgcg cctgcgggct gtagagccag ttcaggtagg cttttgccgc
    14821 tttttccgtg ccgttggcct gtacgttttt atccacccac gccaccggga attccgccag
    14881 aatgttggtt ttcggaatca ccacttcaaa gccctgcgct tcatactgtt tacggatgtt
    14941 gttcacttcc gactcgaagc tgatcagcac atcgcccagg ccgcgctcgg caaaagtggt
    15001 ggtcgcgcca cgaccgccag tatcgaacac ttcaacgttt ttcaggaact gggtcataaa
    15061 ctgttcggtt ttggctttat caccaccgtc ggctttgtcc gctgcgcccc atgccgcaag
    15121 gtaggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct tcacgtcaga
    15181 gcgtaccagg tcgttccagt cgtggatatt ctttgggttg cccttacgca ccaggaagcc
    15241 catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt ccgctgggat
    15301 cagcttgcct ttatcgtgca ggatttgtac gtcggtcacc tggttataag tgacaacgtc
    15361 ggcttttaaa ccctgcaaaa ttgccagcgc ctgttttgat gatccggcat gagattgttt
    15421 aatcgtcagt ttgtcgccac cgttatcttt tgcccattgt tgctcaaacg gcgggttcag
    15481 ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg ttgcctgtac
    15541 atggcccgcc agtagcagag aagcaaccag cacgagtgag ttctttttca gtaagttaac
    15601 ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat ttataacgga
    15661 cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttgaa aagggaataa
    15721 gacgaaatcc tccccacaaa tgcagggagg aaacagaggt gaatcagatg ccgacgctaa
    15781 ccgtctccgg cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg
    15841 attcatccga tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac
    15901 gcatcgggat tgctttcgcc atttcagtca gcaccgattc tggatcttcc gggttcgact
    15961 ggcgggcaat gctttccgcc attggcgtgc gaacgtatcc agggcagatg gcgttaacgc
    16021 gaataccaga ctgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt
    16081 tcgttaaggc gtacgccgtt tcgcccgggt ctgccaccat atcgccagtg actgaagaca
    16141 tcatcacaat ccggccatct ttgcgggcaa tcatctccgg cagcaccgcc ttcgtgacgt
    16201 tccatacgcc ttttatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcaa
    16261 ggaaactgcc cagacgacaa acgcctgcgt tattcaccag aatatcaatg cgcccttctt
    16321 tttccttagc gcgcttaata gctgcggcga ccgacgccgg gtcacgcaca tcggcgacaa
    16381 ccgccgtaca gcgatgacca cggccacaca gttcgtccgc cagcttttcg atctcagggg
    16441 agatatccag caagattagg tttgcgccat gacgcgcaaa agttctggca attccttcgc
    16501 caattccctg caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa
    16561 tgctctcctt gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca
    16621 aaatattgct gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat
    16681 gcatcatttc aggcgttgcg ttagctcact gctgcgctca atcattttca atgactccac
    16741 atcattgagt tgcacaaggc caacaaacag taaatcggta acagaatttt gcgccgtgcg
    16801 ggtggacatc gacgagctgc gccattcggt ttcgccagaa acggtatcga gggtgtaatg
    16861 cgccagccgc cgcagcgggg aatcggttaa tgaggtaatg gcaatcaccg ttgccccctg
    16921 ctttcgcgct gcctcggcgc acaagacaat ctctttttta ctgccactgt aagagatagc
    16981 gatttgtaca tcgccttttt ttagcgcctg agaaacggtg gcttgtacgt gggtgtcggc
    17041 ctcacaggcc acgcgataac caattttcat cagtttgaac gacagatcgc gccccaccag
    17101 ctccgacccg cccaggcccg ttatctggat aaacggtgct ttactgatga cctcaatgat
    17161 tttttgcagc cgcgcgtagt caaacaacgc gcaggtttgc tccagcgcct gctctttttc
    17221 gcgattcagt ttacgggcaa tcacttccag cgaatcatca ctggtaatcg aactgtgcag
    17281 gtgcagcgcc gtggcattcg ttttctcccg gctggcactg tattcgccaa ttaacgccat
    17341 acgcaattca gtaaatcctt gcgcgccgag cttctgggca aacttcacaa tactcgactg
    17401 gctgatgccg agctgtttcg ccatcttgcg cgaagagacg gatttcagct cactcacgtt
    17461 ggcccgcaga aagtcagcaa ttttttgctc gttcccggta aattcacttt ccgcattgcg
    17521 tattttcgtc aggtacaaca tcttccactc tcctgggctg gcagcaaaaa ttccgcttaa
    17581 gttcttatga cgctcttcac actctgcgag tgtagactca atgattcctt taagtctgtc
    17641 aattcgggat atatgattcc atacatctgc aattatttga tcgtaaatag taaggtcacc
    17701 accgatgcaa cttgaaaaga tgattaccga aggctcgaac gccgcttcgg ctgaaattga
    17761 ccgcgtatca acgctggaaa tgtgccggat tatcaacgat gaagataaaa ccgtaccact
    17821 cgccgttgag cgcgtactgc cggatatcgc cgcagcgatc gatgttatcc acgcccaggt
    17881 cagcggcggc ggtcgtctga tttatctcgg tgcgggaaca tcaggtcgtc tggggattct
    17941 ggatgccagc gaatgtccgc cgacctacgg cgtgaaaccg ggtctggtgg ttggattaat
    18001 tgctggcggc gaatatgcca ttcagcacgc ggtggaaggc gcggaagata gccaggaagg
    18061 cggtgtgaat gatctgaaaa acatcggatt aaccgcgcag gatgtggtgg ttggtattgc
    18121 cgccagtggt cgcacgccgt atgtgattgc cggactggaa tacgcgcgcc agctcggctg
    18181 ccgcacagtg ggaatttcct gtaatccagg gagcgcggtt tccaccaccg ctgagtttgc
    18241 tattacgccg atcgttggtc ccgaggtggt taccggttct tcacgaatga aagcaggcac
    18301 agcgcagaaa ctggtgctca atatgctctc caccgggctg atgattaaat ccggcaaagt
    18361 gttcggcaac ctaatggtcg atgtggtcgc caccaacgaa aaattgcatg tgcgccaggt
    18421 caatatcgtc aaaaacgcta ccggatgtag cgcagagcaa gcggaagcag cgttgattgc
    18481 ctgcgagcgc aactgtaaaa cggccattgt gatggtgctg aaaaatctcg atgccgcaga
    18541 agctaaaaaa cgcctggatc aacacggcgg ctttattcgt caggttttag acaaggaata
    18601 acccatggcc aaagagatca gcagtgaact tctgaacacc attcttaccc gggtcggtgg
    18661 accgggaaat atcgccagtt gtggtaactg tatgacgcgc ctgcgtctgg gtgtacatga
    18721 cagttcactg gttgatccca atatcaaaac gctggaaggc gtgaagggcg tcattttaac
    18781 cagcgaccag gtgcaggtcg tttttgggcc tggtaaagcg catcgtgccg caaaagcgat
    18841 gagcgagctg ctgggagaag caccggtaca ggatgccgca gaaatcgccg cccagaacaa
    18901 acgtcagtta aaagccaaac aaacctccgg cgtgcaacaa tttctcgcca aattcgccac
    18961 catcttcacg ccgctgatcc ccggttttat tgccgctggt ctgctgctgg ggatagcgac
    19021 gttaattgcc acggtgatgc acgttccggc agatgctcag ggcacgctac ccgatgcgct
    19081 gaattttatg aaggtgttca gcaaaggcct gttcaccttc ctggtgattc tggtgggcta
    19141 taacgccgcc caggcatttg gcggcacggg cgtcaatggc gcaattatcg ccgcgctctt
    19201 tttgctcggt tataacccaa ctgcaaccac cggttactac gccggatttc acgatttctt
    19261 tggtctgccc atcgatccgc gcggcaatat tatcggcgtg ttgattgccg cgtgggcctg
    19321 cgcccgcatc gaaggcatgg tgcgccgctt tatgccggac gatctcgaca tgctgttgac
    19381 ctcgttaatc accctgctaa tcaccgctac gcttgcgtac ctgatcatta tgccgctggg
    19441 cggctggctg tttgaaggca tgtcgtggct gtttatgcac ctgaacagta atccgctcgg
    19501 ttgtgcagta ttagccgggc tgttcctgat cgccgtggtg tttggcgtgc atcagggctt
    19561 tattcccgtt tacctcgcgt taatggacag tcagggattc aacagcttat ttcccatcct
    19621 ttcaatggca ggcgcgggcc aggtgggcgc ggcgctggca ctctactggc gggcgcaacc
    19681 gcacagtgcg ctacgcagcc aggtgcgcgg ggcgattatt cccggtctgt tgggcgttgg
    19741 cgaaccgctg atttacggcg tcaccctgcc ccgcatgaaa ccgtttgtta ccgcctgttt
    19801 aggtggcgcg gcgggtggtt tgtttatcgg gctgatagcc tggtggggcc tgccgatggg
    19861 cttaaacagt gcctttgggc cgtctggtct ggtggcgctg ccgctgatga catcagcaca
    19921 aggtatcctt ccggcaatgg cggtttatgc aggcgggatt ctggtggcat gggtttgcgg
    19981 gtttattttc accacgctct ttggctgccg taacgtcaat ctggactgat aatatgaaac
    20041 ggacaatgct ctatctttct ctgctggctg tcagctgtag cgtcagcgcc gcaaaatacc
    20101 ctgttctgac agaaagttcg ccagagaaag cagggtttaa cgtcgaacgg cttaatcaga
    20161 tggatcgctg gataagccag caagtggacg ccggttatcc cggcgtaaac ctgctgatta
    20221 taaaagataa tcagattgtt tatcgcaaag cctggggcgc ggcgaaaaag tacgatggca
    20281 gcgcgttaat ggcgcagcct gtcaaagcca ccaccgggac gctgtatgac ctggcttcca
    20341 ataccaagat gtacgccact aacttcgccc tgcaaaagct gatgagcgaa ggcaaactgc
    20401 atcctgacga tcggattgcg aaatatattc cagggtttgc cgacagcccg aacgatgcga
    20461 tcaaaggcaa aaacacgctg cggatttctg atctgctgca tcacagcggc ggtttcccgg
    20521 cagatccgca atacccgaat aaagcggtcg cggacgcgtt atattcccag gataaaggcc
    20581 agacgctgga gatgatcaag cgcacaccgc tggagtatca accctgcagc aagcatatct
    20641 acagcgatgt cgattatatg ctgcttggat ttatcgtcga gtcggttacc ggtcagccac
    20701 ttgaccgcta tgttgaagag tcgatttatc gcccgctcgg cctgacgcac acggtattta
    20761 atccgctgct gaaaggcttt aaaccacagc aaattgccgc cacggaactg aacggcaata
    20821 cccgcgatgg cgtgattcac ttcccgaata tccgcacttc cactctctgg ggtcaggtgc
    20881 acgatgaaaa agccttttat tcgatgggcg gcgtttccgg gcacgcaggt ttgttttccg
    20941 ataccggcga tattgcggtg ttaatgcaaa cgatgctgaa cggcggtggc tatggtgatg
    21001 tgcagctgtt caatgcggaa acggtgaaga tgttcaccac cagctctaag gaagatgcca
    21061 cttttggcct tggctggcgc gtgaatggta atgccaccat gacgccgacg tttggcacgc
    21121 tggcaagccc gcaaacctac ggtcacactg gctggaccgg gacggtgacc gttatcgatc
    21181 cggtgaatca tatggcgatt gtgatgttaa gcaacaagcc acattcgcca gtagccgatc
    21241 cgcaaaagaa tcccaatatg tttgaaagcg gtcagttgcc gattgcaact tatggttggg
    21301 ttgttgatca ggtgtatgcg gcgttaaagc agtaataaaa aggccagtca gttgtgatac
    21361 tggctggcct ttaatcatcg aatcagaaac gcttacaacg ccattaactt gtccagcgac
    21421 ggcgcgaagt aatagccgcc agtgaccggt ttggtaaaac gcaacatcgc atcacgtttg
    21481 ccatcggtat cgccaaacat gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag
    21541 taggcgcaga agtacagacc gtgcgtaccg ctggcggtgc cgtacggcag gctctgtcga
    21601 acaatcttca gccctttgcc atcttctttc agatcaacgc gggtcaggtg agaggtttcc
    21661 ggacgttcgt cgccgtcaat ctcttcgttg gcctctttag tgcgcccgat catcatctct
    21721 tgatcgtgaa tgctcatccg gttgagttgc ttcaggttgt gctcccaacg ctgcacaaac
    21781 acatagctgc cgccggcatc cacgccatct ttgataaccg ccacttcgcg gcgcgtctct
    21841 tcacccgccg ggttttctgt accgtcaaca aagccgctca ggtcacgctc ttcaacccaa
    21901 cggaagccgt ggatctcttc tttcacttca atgcaatcac caaacgcttc catcgccgcc
    21961 tgggcgacag agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac
    22021 tgggtcgtcg gtgcaagacc tttaccgtag cccggaaaat ctttcagctc ttcggcccca
    22081 acgccgccgc tcagagcgcg ccaggtattg ttaccaaagg caaccaccgc accaagatgc
    22141 gcgtccggga atttcgcttc aaaagttgcc agcttgtcgg caaatgtttt actggccgca
    22201 cgcagggcgt caacttcccc tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt
    22261 tctggcaaaa tgccactctg aacctgagac attgttcctc ctgaaaataa gaatgccacg
    22321 caatccgtgg cattgttggg cgctatttta cgtgtttttt cgcggcggcg gtttgttcag
    22381 acgcaaatta acgccgccag ataattttac tgactttcca gtttttcagg gtatcgtccg
    22441 aaggcattaa cccttcagga ccactccatt ccccgctgaa ctggtagcta atatgttgac
    22501 tgccttcggc tttacattcg acggcacgat tatcatcacc atcagctttt tggcaattgc
    22561 caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag
    22621 gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca
    22681 tcgccacgtt gtcgcccttc atcacttcaa aaaagcgcac cacgttgccg ttcgcggttt
    22741 tcattccgct gcgcaggcga taatcgccat caagcgcatc ggcaatggct tgttcttgca
    22801 gtggtgtgga cgccgttaat tcaccaacgc cctgctcgct cactttggtg gatgatccaa
    22861 accagttcca cggattagcg gcagaccagt taacggacga catcgtggaa cagccggtca
    22921 gcatcaacgg catagcgcat aacattaaac gcagcgattt catgtcactt cctttggtta
    22981 ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg ccgttattct ttttcttcat
    23041 taaaacaagc gcgtaaacgt cgattggtca gcagccagat taacgccaca atatccgcca
    23101 ctaccagcgc cagaccgata ccgctaacgg attcaccgtt cagccacaac cacggctgcc
    23161 agcaaagtaa aaccacttgc gccaacagca acagaaaata gagcacatgc cagatacggg
    23221 gaaatgaagc ccgccgacca ctcagcaaaa acgccagcac cgccggaatg ccgggaatca
    23281 accccagcca gaaattatcg tgatcgggat aaaacagatt tagcagtgca gtaccctgct
    23341 cgcgcgacgc accggcaatg acaaacagca cccaggttcg cgcctgaagc aatagcacaa
    23401 gccagaagag caagggtaaa cgcaggcgac cgtacgcatc ataatggaca ggatgaaact
    23461 cagtactctt catcttcaat caaacgctta cccagactca gcacgtcggc gtgttcatat
    23521 cccaggcgtt catacattcc gagcaccatg tcgttatctt ccggcacgtt gatctgaatt
    23581 ttcgggcagc cgcgagcaat cagctttttc tccaaccgat taagcaacgc attagcaatc
    23641 ccacgcccac gaaactctgg atgcacgcca agataataag cagacccgcg atgcccgtca
    23701 taaccgccca tcaccgttcc gaccacttca ccgttcacct cagcgaccag aaacaaactg
    23761 acgtcatggt tcatcttacg ctcaatgtcc atttccggat cgttccacgg acgcagcaag
    23821 tcgcaacgct cccaaagggt gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc
    23881 tccatggtat tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa
    23941 ttagccaata tctgacgaaa atcggttgaa aaagtggcat aatggggagt tgtcaactat
    24001 tgaaatgaaa agtaaaacaa ttctcaacag caaacggtcg taacggatta cgcgatacga
    24061 tataacatct ggaactttat tattacaact caggccgtat gagcactttt aaaccactaa
    24121 aaacactcac ttcgcgccgc caggtgctga aagcaggact tgcagcgctg acgttgtcag
    24181 gaatgtcgca agccatcgcc aaagaagaac ctttaaaaac cagcaacgga cacagcaagc
    24241 cgaaagcaaa aaaatctggt ggcaaacgtg tcgttgttct cgatccaggc cacggcggga
    24301 ttgataccgg agcgatcgga cgcaacggtt caaaagaaaa acatgtggtg ctggcaattg
    24361 ccaaaaacgt ccgttccatt ttgcgtaatc atggcattga tgcgcgttta acgcgttctg
    24421 gcgatacgtt tatcccactg tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc
    24481 tgtttatgtc aattcatgcc gacggcttta ccaacccgaa agctgccggt gcttcggtat
    24541 ttgccctctc taaccgtggg gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa
    24601 accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa ggatcaccta ttgcagcaag
    24661 tgctgtttga tctggtgcaa accgatacta ttaaaaacag cctgacgctc ggctcgcata
    24721 ttctgaaaaa gattaagccg gtgcataaac tgcacagccg caacaccgaa caagccgcgt
    24781 ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg tttatcacca
    24841 acccggaaga agagcggctg ttaggcacgg cggcatttcg ccagaaaatc gccacagcga
    24901 ttgccgaagg cgtgatcagt tatttccact ggtttgacaa ccagaaagca cactcgagaa
    24961 agcggtaagt gatgaaaccc gacgcacacc aggttaaaca gtttctgctc aaccttcagg
    25021 atacaatttg tcagcagttg agcgccgtcg atggcacaga atttgtcgaa gatagctggc
    25081 agcgcgaagc tggcggcggc ggacgtagcc gggtgttgcg taatggtggt gttttcgaac
    25141 aggcgggtgt caacttttca catgtccacg gtgaggcgat gcctgcttcc gccaccgctc
    25201 atcgcccgga acttgccggg cgcagtttcg aggcgatggg cgtttcactg gtagtgcatc
    25261 cgcataatcc gtatgttccc accagccacg cgaatgtgcg cttttttatt gccgaaaaac
    25321 cgggtgccga acccgtctgg tggtttggcg gcggcttcga cttaacccct ttctatggtt
    25381 ttgaagacga tgctattcac tggcaccgcc cgtgacctgt gcctgccatt tggcgaagac
    25441 gtttatccgc gttacaaaaa gtggtgcgac gactacttct acctcaaaca tcgtaacgaa
    25501 caacgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt cgaccactgt
    25561 tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc aattgtagag
    25621 cgacgtaaaa cgatggccta cggcgagcgc gagcgcaatt tccagttata tcgtcgcggt
    25681 cgttatgtcg agttcaatct ggtctgggat cgtggcacac tgtttggcct gcaaaccggc
    25741 gggcgcaccg agtctatcct gatgtcaatg ccgccgctgg tacgctggga gtatgattat
    25801 cagccagaag atggcagccc ggaagcggcg ttaagtgagt ttattaaggt cagggattgg
    25861 gtgtaactcc ctccccccca ttcccgcatc cgctgatgca gcgtcagtga cggcttctcg
    25921 gcaaacagct gctggtaatc cgtggcgaat tgccccagat gccagaatcc ccactgcatg
    25981 gcggcgtctt ttaccgttgt gctttgcgac caagggctta tcagttcgcg gcgtacggcg
    26041 ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc ctaaaatagc gtgaaacgcg
    26101 ttttgtagcg tgcggcggct gacatgcagt tgattacaca agtccagcac cgtcactggt
    26161 tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaatcgacg gtaactctga
    26221 tgactgatgc tttccgccgt caccattggc tgcgcttctt ccagcatcgc ccccatcgcc
    26281 attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt ttccgggttt
    26341 tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gagcggcttt atgctgctct
    26401 ttcacttcca acgccgactg gctacgcagc atatgtaata cccgatccgg attatgcaaa
    26461 aagttagcct gacgagtgat gacatcttca gaaagcacta cgccaaggat cgtgtaatcg
    26521 tccggcgtac ttaattcaaa ctcggttcca ccggggcgcg tcgctatttc tgcgcttccc
    26581 agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc aaaccagaac
    26641 gagttcggcc agaccaggca cgactggcgc agcgccagac cggtgtattc acggaaaacc
    26701 tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg atgcagctga
    26761 tcgtaaatct gctgccaggc ggtaattgtc aaagcatgtt catagacatc cgttgtctgt
    26821 cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc gggtaagggt
    26881 tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt aatgccgggt
    26941 gttgtaggac acccggcacc tccgacaggt taatggggct tgagacgata acgactactg
    27001 cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc tttttccagc
    27061 ggccagccaa agtgggcagc cacttctcct gccgtcattc cctgacgtac tgatgttaac
    27121 agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg ttcctttacg
    27181 ggttgcttag gctggcggtt aaagccagta accagccact gggtgtcatc gtctggccga
    27241 ccgatctcct tacggctgat gacacgcccg gtacgcatcg cggcagcaca acctgcgtcc
    27301 agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaggcg accagggtca
    27361 agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc agctgcatct
    27421 atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat cggcaacctc
    27481 cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt aaaggcgtta
    27541 caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta gttggtttca
    27601 gacggtggcg ggacataggt caccagctgt acgtcggcag atttcagggc tgcatcaatg
    27661 ccgtaggtcg cttccagcgg cggtgccacc agatacgcca tcggatcgcc aagcgtgatc
    27721 ccggcggttg atgagagata agaaccggta cgcgaaacta catgtgccag gaaggctgtg
    27781 ttttctgcgt cattagccca ctggaaagca gcgccatttt caatatgcgc aaccatcgca
    27841 tccagaccag cacgcacttc cgccgggttt ggcccaccaa gcataatcag cacttcaccg
    27901 gcagtcggtg acgggccgtg tgcggctccc gcatacagcg agcggccata caccacttcc
    27961 accatcgcct gcttggtcgc ttcgtcagcc gcaatgtagg tgacgtcatc tgaatcagca
    28021 gaaatcagcc cgaggctacg aatatgcggc ggcaatttca gttcacgcgc aaattcggcg
    28081 ttaacggagg caatcacccg catggcggtt accgacggtc gaatcaaatc taaagctggc
    28141 atgatgcctc cttatcgggt catgttgatg ccggatgctt tctgctccag catacgtttc
    28201 gccaaatcca caatgacggc tgcggcttca accggcggcg tgccgccctg gtgaatgtta
    28261 gagatacagg tacggtcggc ctcaacggtg gttgccatac gcggcgagta aacggcgtag
    28321 caggagaggc tttctgactg ccctaatccc gggcgttccc ccaccagtag gatcactact
    28381 ttcgcgccga ggatctcacc gatctgatct tcaatcttca cgcgaccata acgaacaaag
    28441 aatggtgtac cgactttcag cccggcctgt ttcaggcccg ccatcagcgg tggcaggatc
    28501 tcttcgtagt tcacggtgat cgcatcagtt gacaggccat cagaaataac gacctgtacg
    28561 tccggattag caacacactg tgctttcagc gcttcaacag cttctgcaca caggcggcgg
    28621 cccatatccg gacgagtcag gtagagattt ttgtcgctaa tttcagagcg gacttccagc
    28681 aagccctgcg ctttcaccca ctcttccggt acttctttca gtacggtatc tttcgaacga
    28741 gagtgatcgg ccaggaaacg cagcaacgcc tgggtacgcg gacgcggacc ggcacgaccg
    28801 gtacaaacgc gggccacggt gctgcgacgc agttctgtta atacgtctgc gcgatgcggg
    28861 ttttccacac caatccacgc ttttgcttcc gcagaaccta aatccagcgc gcagctttcc
    28921 gaggtcactg gtgtcgtaca ggtggtggtg gcgcactttg cttctgacgg cgcgggggcc
    28981 gcttgtccca ttgacgccat cacgctgcgt acaatttctt caatctgttt ttgatccatg
    29041 atgtgttatc tccgcgtcat cagaagaaca gtgacggatc gcccgcccgt ttggtcaggc
    29101 gaccgtttgc cataatgccc atgctttcca gccagcgttc aaactccggt gacgggcgca
    29161 ggttgagtaa ctgacgcaca gtggcggtgt cgtggaaggc cgtagtctgg tagttgagca
    29221 tgatgtcatc acccagcggc atccccatga tgtagttaca gcctgcggtg gcgagcagga
    29281 tcatcaggtt ttcgttgagg ttctggtcag cgtcagcgtg gttggtgtag cagcagtcac
    29341 agcccataga gatgccgctc agcttgccca taaagtgatc ttctaagccc gcgcggataa
    29401 tctggcggtc gttgtagaga tactccggcc caataaaacc gaccacggtg ttgacgataa
    29461 acggatcgta atgacgcgcc agcccatagt tacgcgcttc catcgtcacc tggtcagcgc
    29521 cgaagttagc gccagcggac agcgccgaac cttgtccggt ttcgaagtag aggcagtttt
    29581 ccccggcgat acggttaaac tctgcaccca ctgcgcgcgc ttcgtcgagc atcgccagct
    29641 ccacgccaaa ctctttcagc cctttttcgc tgccgcaaat actctggaaa atcagcccgc
    29701 ccggtgcgcc gcgacgaatc gcttcgatct gggtggtgac gtgcgccagt acgcagccct
    29761 gagttgggat gttgaatttg tcgatcacgc catagatggt atccaacacg cggcttaagt
    29821 tttccacgtc gtcagtcacc gggttaacgc cgatcaccgc atcgcccacc ccgaaggaaa
    29881 gcccttcgta gatttgcgcg gcgatacttt gcacatcgtc acgggtatcg tttggctgca
    29941 agcgggcgct aaaggtgccc ggaataccga tggtggtatt ggcctttttg atcaccggca
    30001 ttttcttcgc gccgtagatc aggtccgcgt tggagcaaat cttcgctacc gccgctacca
    30061 cttccgaggt cagccctttg cgggtaaagg caatgtcgtc cacgctggtt tcatcgctca
    30121 gcacatactc acgcagttcg ctgatgctcc agtttttaat ctggttgtag gccgtttcgt
    30181 taacatcgtc ctgaatcagc cgcgtcacgc agtcatcttc ataggcaatc accggattat
    30241 tgcggatgtc cgctacggtc atttccgaca acacctgctt tgccgccacg cgctcctgtg
    30301 agcttgccgc agcaacgccc gccagcacat cccccgaacg cagttcgttg gctttagcca
    30361 gcacctcttt tacatcctta aactgatata cattgccgaa caatgtggtc tttagtttca
    30421 taagtcgttc cctcaggaag gaaatgcgag tgatttcacc gtcaccggca caaccgatcc
    30481 gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca cttcgtcaat
    30541 gactgccaac gggagttgtt gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa
    30601 gtcctgcccg gccaccacca gcaggggatg cggattcgga aaacgcgcga cgaaatcgac
    30661 caacgcgttg atgaccgtca gtaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc
    30721 cagcacgtac gcgtcagttt taggatcaag atccagctga atcagcgcct gttgccaggc
    30781 actcaccaga tccttttcat caattgggat cgccaccggc aagttgcgca gcggcagttg
    30841 tacgccctcc agccagattg tgctgccaga gagcgaaagg gtatgtgcac ccgcgccaat
    30901 caccgtggcg cgcacggttt gcgccggaaa ctgcacgttc atctcacgca ggcgcggatg
    30961 gtcatgcagc gccgttgcca gaagcgggcc aatatcggca aaacagaacg ggtcggcggg
    31021 ctggtggcga taacattcgc caacgccgcc agaaagcgta atgatttcgg gcgtaacacc
    31081 tacgggcagc aaaccggttt gcatcaatgc ctgcgcgagc ggcgaaagcg ttccgtcaat
    31141 cacttcgaca atcagctctg ccatccgccg ggtaacctgc accagctgcg cgccggtcag
    31201 cgaacgggct tcggtgcctg caccgaagca ctcatccaca atcatctgcc ccggtttatg
    31261 agcgtaaacc acacgcccct ggctgtcggt ttccagcagg cgaccgccga cattgaggca
    31321 ggcggtgccg ctgatttttc cggcatcgaa cagggcgtag ttcgcggtgc caccgccgat
    31381 atcgatattc aggacccgac acagccgttg ttcagaaagg gtttgcgccc cggcaccgtg
    31441 accggcgatc acggattcga gatgcggtcc agcgctggcg acgacaaaat cgcccagcga
    31501 ctgggagagc gtcatcaccg ccgggcgagc gttgcgggtt ttcgcgcttt caccggtgat
    31561 gatgatggca ccagaatcaa cgctttccgg cgcaatgccc gcagcctgat attgctcgag
    31621 gattaaggtt ttcagttccg cttcttttaa accaccctgt ttatcgacag gggtaaagaa
    31681 caccgggctt tgccagctaa tttcgcgttt gatgaattcg tagcgcggca cctgcgacac
    31741 cgccgcacgg ttaaccagct ccaggtggga gaaaatcacc tgggtggtgg tggtgccgat
    31801 atcgataccg acgctcagta gctggcgagt gttcacgatt gcgcctccgc ttcggtttta
    31861 gccgcgctcg cgtcttcttt aggtaccagc atcatcgcca cgccaatcgc cgtgacgccg
    31921 ccgatcagct tgccgacaat catcgggaag atcatggcgt tcatgttggc agccgcgaag
    31981 cctaaatggt cgcccagggc gaaagcagcg gaaacggcga aggcacagtt gatgactttg
    32041 ccgcgggtat ccatctgctt catcatgcca aacatcggga tgttgttggc aagcgttgcc
    32101 accatgccgg ctgccgctat attgttcata ttcagcacct taccgacact catcagcggt
    32161 ttttcaaacc agcgagtcag cagcagcacc atcggatacg cccccagcag cacgcaggag
    32221 atagaaccga taacttcaat ggcgcgcatc acttcaccgg gtttgtcgcc tggagccatg
    32281 aagatcggat ccagaccggg gatcagttcc caaccgagca ggaatttcac taccgcagcg
    32341 gcaagaccga gggtgatcaa tgcaacgagg aatttggcga agatctggaa gccgttgatc
    32401 attttttccg ggatgaattt cagccccagc gccaccagca ccgcaacgat aagtaccggg
    32461 atcatgttca tcaggatcag cgcaaaggtg aattccaccg gctgaccgtt gatctgcaca
    32521 ccggagtaca tagcaaccag accaccagca atacagccaa tcggaatggt cacaatgccc
    32581 gccagcacgc cgagcgccag ataacgacgg tcagaaggtt cgataatgcc gagcgccacc
    32641 ggaatggaaa acacaatcgt tggccccatc atcgacccga gaattaaccc agagtatagc
    32701 cacgctgcta cgtcgccgcc cgccagctct ttggcgagga agaagccgcc catatcgcac
    32761 gccagcagtg ttccggcgaa catcgatggg ttagcgccga gcatttcgta aaccgggata
    32821 atcaccggcc cgagtacgtg ggccagtacc ggtgccagcg cggtcatacc gaccatagcc
    32881 aggcccaaag cgcccattgc cataaagcct tcttcgaact gaccgccgga tccttcgata
    32941 cttttaccga acttaccgag gaaacgagca gaaccgccga actgcgacag gatcctgtct
    33001 acggcagcta tcagcataaa gaacatcatg atgtacatga tgatttcgtt aatacccatc
    33061 gcctttactc cctgttagtt gttatttatt ggcggatgcg gcgtgaacgc cttatccgcc
    33121 ctacatgtgc aatcccgaag gccagataag acgcggcaag cgtcgcatcc ggcaattgca
    33181 ccgcgccact ggcggatgcg gcgtgaacgc cttatccgcc ctacatgtgc aatcccgtag
    33241 gccggataag acgcggcaag cgtcgcatcc ggcatttatt gcgccgctgc gtacaggccg
    33301 acaatctgct ccaggctggc ggtacgcggg ttactgcgca gacaaatatc ttccagcgcg
    33361 gcctgcgccc atgcgccgta atgcgcagat gtcgcaccaa catcgcccag tcgtttacca
    33421 atcccaactt ccgctatcag ctcacttacc gcattaatag cgtcacgatc gtcggatttt
    33481 ttagttcgca gcgcccgacc aaccaagcta aaccgatcac gacaaaccat ccggttaaat
    33541 tccatcaccg ttggcaacaa catggcgttc gccagaccgt gcggaatatg cagcgccgcc
    33601 cctggctgat gcgccatcgc gtggcacaac ccaagtccgg cactggaaaa cgccattccc
    33661 gccatacatg aagccagcaa catgctctcg cgcgcggcaa ggtcatgacc gtagcccacc
    33721 gctttcggca gcgatttgcc aatcatcgca atggcaccaa tcgccagact gtcggtaaac
    33781 ggtgtggcgt tcagcgcgct gtacgcttca atggcatggg ttaacgcatc aatgccagtc
    33841 atcgccgtga catgcgacgg cacaccttcg gtcaatgcgg cgtcgagaat cgccacatcc
    33901 ggcatcagcg aggcatgggc taacacctgc ttgcgcccgc tcaccgcgtc gataatcacc
    33961 gttacgttgg tggtttcaga gccggttccg gcggtcgttg gaatggcaat caacggcagg
    34021 cgcggttgca gaacgctggt ttctgacatt tctgccagcg tgctatcggg gttcgtcacc
    34081 agcaacgcca cggctttcgc cgcatccagc accgagccgc cgccaaacgc gatcaccccg
    34141 tcacaacctg attcacgcaa ctgcgccacg gctgcacaca cgtcggtgat gcacggttcg
    34201 cccaccggac atggccagag cgtcatggcg atacctttaa cggccaggct acgcgtcagc
    34261 ccggcggtca tccccgcctg atgcaaaaag ctgtctgcca tcacgaacag atgtttcagc
    34321 ccacgcgttt gcgcttgctg cccacaactg ctcaccgcgc ccggaccgca aagcgtcacc
    34381 ggtggaacgc taaatgtttt cacccgctgc agattcaggg tatcgaatgc ctgaaaaagc
    34441 gccgtttgca attcactttg catagatccc ctccgttttc tctcgcccac tactggcgat
    34501 cgccagcggg gtcataaaca aactgtgctg cggtaaatgc acctgtaacg ccgggaattg
    34561 tttgcgaaac agttccgcta cgcccggttg catacaggaa ccgcccgcca gccataaatc
    34621 cgtaatgccc tgcccttcaa tatgccgggc cacaatgtcc gccatttttt cgtacaccgg
    34681 tttcaccgcg ggccaaatct cgtcaccgtg accgcgtttg tactgctctg cctcttccag
    34741 cgaaatacgg cgatttccgg cgagagtcag agagatgtga tgtccgccgg tggcttcatc
    34801 cgccgagtac gtcactttgc cctttttcac gatggcaatg ccggtagtgc cgccgccgat
    34861 atccactaca ccggcgttgt ccagttgcag cagatccgca actgcggttg gctcatccag
    34921 cacatggctc acttccagtc cagcagactc cagcacgttg atggaaatac gcgggtccgt
    34981 acctggagga aaagaggtcg ccgcatggct aaaacggcga ccgaattgct gctcgagggt
    35041 gtcgagatgg cgacgaacaa tggtgaccgc accgaagaaa tcccagacga tgccgtcgcg
    35101 gaccacatca gcccagtcga ggcataccgc caccggctga ccgtcgcggt cgacaaccat
    35161 cgacaccaca tcgcaggtgc ccagatccac ccccagccac agcggagatt ccgtcgcggc
    35221 gggcgtctgg ttacacagcg ttgccgccgt ttgcagacgt ggggtgagcc attgttcgtc
    35281 gtgcgccatc tgttactcct taaacaatgc gaaacgcatc gactaataca cagcgacgca
    35341 gacggacaaa cgtccgcgcg ctggttaccc cttcaccggt tggcgtggtg atggtcatag
    35401 tggtccagcc ttccccgccc agccccagcc cggcaatgca cggtccgttc ttaacgaaaa
    35461 tgctggtatc aatggcattc gccatctggt tcatgttctc gatattgcgc gagtgcattg
    35521 ccgccgtgtg gtggcaaccg ccttccagtt tcaccgccag cgcaatggca tccgccacgt
    35581 tggcgacgcg cacgacgggc agcaccggca tcatcagttc agtcacagca aacggatgtt
    35641 ctgcggtggt ttctacaaac agcagtcgcg tttgttccgg aactttaagg ccgattgccg
    35701 ccgcgatttt cgccgcatcg cgaccaaccc agtcacggct gacggtgcct tttccgcgct
    35761 cgtcgatatt tttcagcaac accgattgca gttgctgcgc ctgctctgcg gtcagtttca
    35821 ccgcatgctg gccttccatc agacgcatca gttcatcggc tacgctatca acaacaatca
    35881 gtaccttttc gtcggcacaa atgatgttgt tatcgaaaga agcacctttg acgatggact
    35941 gagctgcacg ggcgaggtcg gcggtttcat ccaccactac cggcgggtta ccggcacctg
    36001 ccgcaatcag gcgtttattg gtgtgtttgc gcgccgcttc cactaccgct tcgccgccgg
    36061 tcaccaccag caggccgata cccggaaact tgaacaagcg ttgcgcggtt tcgatatccg
    36121 gatttgctac ggtaaccagt aagttttccg gcccgcctgc ggcaacaatc gcctggttga
    36181 gcagcgtaat cgcccgctgg gagacttttt tcgccgccgg atgcggggca aaaatgacgc
    36241 tgttgcccgc ggcaatcagg ctgatggcgt tattaatgac ggttgccgcc gggttagtgg
    36301 aaggcgtcac cgaagccact acgccccagg gcgcgttttc aattaaagtc aggccgttgt
    36361 cgccagtcag cacctgtgga gagaggcact caacgcctgg tgtgccgcgc gcctgagcga
    36421 cgtttttggc aaatttatct tcaacgcgcc ccatgccggt ttcactgacg gcaagttccg
    36481 ctaaatctct ggcgtgtttt tcgcctgctt cacgaatggc agcaatggct aactggcgca
    36541 ttgccacgct ttttaaccct tgctgggcga ctttggctgc cgcaacggcg tcatccaggg
    36601 acgcgaaaac gcccatctca tgaacggcgg cgggcggcgt gtcactgctt tgcattttca
    36661 gcagtaccgc tttcaccacc tgttcaatat cctgttgatt catgatgttc tgccttattt
    36721 gtggaaaatg acctgaccgc cagacaccac ctcatcgacg atgccaatca cgcacagatc
    36781 gaccggtgac gtttcgcttt tatgcgcctg gcgggcggaa ctgccactca ccagcaacac
    36841 ccactccccg gttcccgcgc caatattgtc gatggcgacg gcgcattgcc cgtcaggatt
    36901 accttgtgga tcaatcattt ccaccatcag caatttgtca tgcgccagtc cgtgatggcg
    36961 tacggtacaa acaatttgtc cagtgacgac tgccagtttc atacccgcct ccgtggcgta
    37021 tttcaggtaa aagctccccc taccctccgc agaaggtaaa atgaaaaagg agagagcgtg
    37081 acgcccgaat cgacgtcaca cagggtgatt acaggttgct gctatcgcct ttcaggccga
    37141 tcgggaagac ttcttccagg tcgccgtgcg gacgcgggat aacgtgtaca gataccagct
    37201 cgccgatacg ctgtgccgct gcggcacctg catcggtcgc ggctttacag gctgcaacat
    37261 caccacgaac catggcagta cacaggccgc cgccaatctg cttaacacca accagcttga
    37321 cgcgcgcagc tttaaccatc gcgtcagaag cctcaatcag tgcaaccagg ccccgggttt
    37381 cgatcattcc taatgcttcc attgtgcttt cctctttatc agggtccaga acgggaccgt
    37441 tcattcaacc agtgtttgta aactgctttc gcggttcact tctgtctgac gctgcacagc
    37501 tgccaccagc gccagttcga taatttcctg cacgctacaa ccacgagaga gatcgtgcat
    37561 cggcgcggca agtccttgta tcaatggccc gacggcacga tatccgccaa gtcgttgtgc
    37621 gattttgtag ccaatatttc cggcttccag cgacggaaaa accatcacat tagctttgcc
    37681 ctgtaacggg ctggcaggcg ctttttgcgc cgccacttcc ggcacgaagg cggcgtcaaa
    37741 ctgcaactcg ccatcgacaa ccagctttgg tgcgcgctca cggacgattt ctgtcgcctg
    37801 ctggacgttg gcaacgcagg ggtgacgggc actaccgttg ctggaaaacg acaacatcgc
    37861 cacgcgcggt tcttctccgg tgatggcgcg ccaggtttcg gcgctggcaa gcgcgatatc
    37921 cgccagttgc gccgccgtcg gctgtggcac tacgctgcaa tcggcaaagc ccaatgcggg
    37981 gccactgtac tgtggcagca tcaggaaaat ggaggagagc gttttacagc ctggctgtaa
    38041 gccgataatg cgtaatccgg cacgcagcac attcgccgtg gaagagagat tgcccgcgat
    38101 acagacatcc gctgtcccgg cgctgaccat tgcggcagcg aacatcagcg gatcggtgag
    38161 tttatccagc gcatccggcg gcgttttttc gcccgcacgg gccagccagc ggagagcaaa
    38221 ttcttcccgc attgcgaggt tgccatgcgg atcaatcacc tgtagcccgt ccatcgccac
    38281 gccgtgactg agcgcaaact gacgaagttc aaacggattg gcgaccagaa tgggcgttgc
    38341 cagaccttgt tgatgtaaat attgcgccgc tttcagcaca cgttgatcta acgcatccgg
    38401 aaaaactact ctggcgggcg ctcgcaacgc cagtttacga caacgttcaa taatcattgc
    38461 gtttccccca gtcgttgttg gatctgcttc accgttagcg actgcttcgt tacgctcagg
    38521 atcatcatca cgtaaaccgt gctggaaagg cggttcagcg cctgcaagat gtccgggcgt
    38581 aatacttcaa agctgcgggt gataaacacc tgcgccgcta cagtttcggt ttcccgcact
    38641 ttggtgcgta gcagattgag caacgccgca tcacgcccgt ggctggcttc cggcaccaga
    38701 tgatcgtggt cgagatagcg cagcggctgg tgagaaagcc gatgcagatc ttcgtcagaa
    38761 agcccgacaa tcgcttgttc gcccagcggt tcccccagcg catcggcgcg cataatgttg
    38821 ccaagacgcg aacggatatc cgccagccac ggctgccacg gttccgccag ttcaatttgc
    38881 agccacactg ccagcgcaat ggtgctgtcg agaacagcgc gaaaagccag gcgcggatcg
    38941 cttttggcaa ccattttttc cgccgacagg tgagtcagcg tatccggctt ttttgccacc
    39001 ggctggcggc acagttcgca gcacgcctgc ggatgttcat cgctactggt tagcccgtga
    39061 acgggctgcg gctgctgctg ttcatcgtca acaaacaggc ggccctgctc gtcaataaac
    39121 ttgatgcgca gatggcggct ttccagtaac tcccgggcag agggcgtcag gcgactgtcc
    39181 gcagggagat ggatctctgc gccttcgctg agcgtatgat tcgctcttag ccatgcttcg
    39241 gtgatgaaat ctttcataag gattgccagt tagccggcca ggcgacatac aggaatttca
    39301 cgctggatgt cgtaccaaat tcgatgctgg agcctttcgg gataaacatc acatcgcccg
    39361 ctttggcgat catagtttcg ccttcgtggc gaacatgcag ctccccttcc agcaccatgt
    39421 cgatttcgtc gtagttcagg gtccacggaa agaaggcgtt ctcccactgc atgaaaccag
    39481 cggccatact gctgccgtcg tctccggtca cgagatcagt caagcctacg cagtgcggtt
    39541 ccgcgccatc gaaacgacca aacttgacgc tgctgccatc aataactttg atgccgcctt
    39601 tgccggtcac cgatttaaag ctcggctgca gtgcaccctg ctccagcgac tgtttttcct
    39661 tcatcacttt ttccatcagc tgcgcgacca ggctttcggt aaactgacct tccggcagtt
    39721 gggcgataat ggtttcacgg atgcgctggc tttcggtttt atcggctgat gcgctggcgg
    39781 gaacagacgc cgttaccggg atcgattcat cacattcggt gatggtaaaa cccaacagat
    39841 cagccacttc acgagcttcc ggggtaataa tgctggcgcg cagaaccacg gacattgcca
    39901 gttcgccgcg cgcgtgtgct tcacgaatat cattcgctgt gatgagtttt ttcacctgct
    39961 tcctcctgtt tggtgagaga tgccagataa tccaccagtt gctgcacgct ttgcgggtcg
    40021 tggctgttta gctcgaaaat cggctcttcg aagcccgtct caagcaataa ctttcgcgtt
    40081 gcggcgacgt cagcatctgg catgtccgtt ttactgatga cggcgatttg ccgtttgctg
    40141 acgccaatat ccagcaaccc ggcaggcaac cgactttccg ggtcattcgc gccgtggaca
    40201 tagatcagca tgtccacatc ctgtagcgtg gtaattaatg cgtgatacca gcggggatgg
    40261 ctaaaatatt cacccggcgt atcaatatcg ccattatcat taaattccac ggcctgcgtt
    40321 tttctggcga gggtataatt cccctgcaac gcattaaata acgttgtttt tcctgccccg
    40381 acggtaccca caaacgcaat tcgtttcatg aatgcctcat attaactttt ggtcatttca
    40441 caaagcgtat aatttaataa gcgtcctaaa ccactgacgg tttgtgataa cgcttcttct
    40501 actgcgccca ccgaaccata aatcaccagc gcgccgctaa aacggtcgag aaaaccgata
    40561 tgcacatcgg cggctttcaa cgctaaatca ccggcgatca tcgccgtttc tccgggagtt
    40621 agcgtcataa tgccaattgc gcccgcatcc ggaacgccga ttttcttcgc cagttcctcg
    40681 ccgggatgcg cgatgagatg cgccagcgtg acctgtttgc cgggcacaaa ttcctgaatg
    40741 atgcgttctt tatccatgag tcgccgcctg tgaaaaactt tcgtgtatcg tgacaatttt
    40801 tcgtcagagt aaataacaaa attcggcaag cgcgtttaaa aggtgaggta gatcactaag
    40861 aatggagaga tatttactgt tatgaataaa ctttcatatg cgacgcatga atataaataa
    40921 aataatatcg agatatttta tatactgatt tttttattat tcgtaaactc agtaataagc
    40981 tttactaata tattgcagct aaactgctta ccctgaatat tcagggtaag cgtgagagtt
    41041 aaaaaaaaat tacagcggtt gggtttgcgc ttcaaccacg gccagcgcca ccatattgac
    41101 gatacgacgt accgatgcga tcggcgttaa cacgtgaacc ggtttcgcca cacccatcag
    41161 caccggaccg acagtcacgc cttccgagct ggaaacgcgc agtaagttgt aactaatgcg
    41221 ggcagcttcc atgttcggca tcaccagaat attagcggaa cctttcaaag ggctgtccgg
    41281 catacggtca ttccgaatcg cttccaccag cgctgcatcg ccgtgcattt caccatcaat
    41341 catcagttct ggtgcacgtt ccctgaccag ttccagcgcc tgacgcattt tgctcgacga
    41401 cgggcagtca gaagaaccaa agttggagtg cgacaacaga gccacacgcg gctcaatacc
    41461 aaaacgacga acagtttctg ccgccatcaa ggtgatctcc gccagctctt ctgcatcagg
    41521 ctcatcattg acgtaggtat cggcaataaa ggtgttacca ctcggcaaca gcagcgcgtt
    41581 cattgcacct gcggtgtgaa cgccatcgcg ataaccaaag acatttttca ccacgctaaa
    41641 gtgttcatga taatcgccca ccgtaccgca aatcattgca tcggcttcgc cacgctgaac
    41701 catgatcgcg ccgatcactg tcgggttact gatcagcgca cgctgcgcct gctcctgagt
    41761 gacaccgcga cgcttcatga tctggaagta ttcggtccag tactctttaa agcgcggatc
    41821 ggattcgtta ttgacgatct caaaatcaac gcccgccttg atctgcaagc ccagtttctg
    41881 aatgcgcatt tcgatcacgt tcggacgacc aataaggatc ggtttcgcca gtcccagcgt
    41941 taccagttcc tgagtggcat gcagaacgcg cgcctcttcc ccttccggca gaactacgcg
    42001 cttcggcgct ttgcgagcct gggagaaaat cggcttcata aacaggttag ttttgtaaac
    42061 gaactccgtc agcttgtcga tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc
    42121 cgactccatc gccgctttcg ccaccgcagg agcgatctta acgatcaagc gcggatcaaa
    42181 cggttttgga atgatgtatt ccggaccaaa gctcagatcc tgatcgccat acgctgaagc
    42241 caccacttca ctctgttccg catgggccag ttctgcaatc gcgcgtaccg ccgccagttt
    42301 catctcttcg ttgatggcgg ttgcgccaac gtccagcgcg ccacggaaga taaacgggaa
    42361 gcaaaggacg ttgttcacct ggttcgggta gtcggaacgg ccggtacaga tgatggcatc
    42421 cggacgcact tctttcgcca gcggcggcag aatttccggt tccgggttcg ccagcgccag
    42481 gatcattggc gcacgggcca ttttcttcac catttcctgg gtcagcactt tcgggccgga
    42541 acagccgagg aaaatatccg cgccttcaat cacatcatcg agggtacgtt taccgtcatc
    42601 caccaccgcg tatgcggctt tggtttccgc catgtttggc tcacggcccc gatagataac
    42661 gccttttgaa tcgcaaacca cgatgttatg tttttgcaga cccagcgcta ccagcaggtt
    42721 catacaggcg attgctgcgg cacccgcgcc agaaaccacc atccgcacgt cggagatgtt
    42781 tttctccacc acgcgcaaac cgttgaggat ggcggcagtg ctgataattg ccgtgccgtg
    42841 ctgatcgtcg tggaataccg gaatattcat ccgctcgcgc agtttctgtt caatatagaa
    42901 acattctggc gctttaatgt cttcgaggtt gatgccgcca aaggtcggtt cgagcgcagc
    42961 gacgacttca ataaatttgt ccgggtcgag ttcatcaact tctatgtcaa atacatcaat
    43021 cccggcgaat ttcttaaaca gaacgccctt gccttccatc accggtttgc ctgccagcgc
    43081 gccaatgttg cctaacccca gcaccgccgt accgttagag atcaccgcta ccaggttacc
    43141 gcgcgcggta tatttgtagg cttttaacgg gtctttttcg atttcaagac aaggtgcggc
    43201 aacgcctggt gagtaggcca gcgccagatc gcgctgcgtt gccagaggct tggttggaga
    43261 aacctggatt ttccctggaa ctggaaattc atggaaatca agtgcacttt gttttaactg
    43321 gtcatccatt tggttgttcc tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc
    43381 ccctgcccac aacgtggcgc acattattac cttgccggag tctacagact ttgagcaagt
    43441 ccaaactctc accattaata taatgttttg gtaataatcc tataacactg atgttacctg
    43501 cttaatccag caataccatg cctgtctgct atgctttttt gatgcgttta gcgaaatttc
    43561 tcagaagtgt gaattaacgc actcatctaa cactttactt ttcaaggagt atttcctatg
    43621 aacgagttag acggcatcaa acagttcacc actgtcgtgg cagacagcgg cgatattgag
    43681 tccattcgcc attatcatcc ccaggatgcc accaccaatc cttcgctgtt actcaaggct
    43741 gccggattat cacaatatga gcatttaata gacgatgcta tcgcctgggg taaaaaaaat
    43801 ggcaagaccc aggagcaaca ggtggtcgca gcgtgtgaca aactggcggt caatttcggt
    43861 gctgaaattc tcaaaatcgt acccggtcgc gtgtcaacag aagttgatgc acgcctctct
    43921 tttgataaag aaaagagtat tgagaaggcg cgccatctgg tggacttgta tcagcaacaa
    43981 ggcgttgaga aatcacgcat tctgatcaag ctggcttcga cctgggaagg aattcgcgcg
    44041 gcagaagagc tggaaaaaga aggtattaac tgcaacctga cgctgctgtt ttcttttgca
    44101 caggcgcggg cctgtgcaga agcaggcgtt tttctgattt cgccgtttgt cgggcgtatt
    44161 tatgactggt atcaggcacg caagccgatg gacccgtatg tggtggaaga agatccaggc
    44221 gttaaatcag tacgcaatat ctacgactac tataagcaac accactatga aaccattgtg
    44281 atgggcgcga gcttccgtcg caccgaacaa atcctcgcct taaccggctg cgaccgactg
    44341 actatcgcac cgaatttgct gaaggagcta caggaaaaag tttcgccagt ggtacgtaaa
    44401 ttaatcccac cttctcagac gttcccacgc ccagctccca tgagcgaagc ggaattccgt
    44461 tgggagcaca atcaggatgc gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc
    44521 gccgttgatc aacgcaaact ggaagatctt cttgccgcca aactataaac cagccacgga
    44581 gtgttatatg tcccgaaaag accttgccaa tgcgattcgc gcactcagta tggatgcggt
    44641 acaaaaagcc aattctggtc atcccggcgc gccgatgggc atggctgata ttgccgaagt
    44701 gctgtggaac gattttctta aacataatcc taccgaccca acctggtatg atcgcgaccg
    44761 ctttattctt tccaacggtc acgcgtcgat gctgctctac agtttgctgc atctgaccgg
    44821 ttacgacctg ccactggaag aactgaagaa cttccgtcag ttgcattcga aaacccctgg
    44881 tcacccggaa atcggctata cgccaggtgt tgaaaccacc accggtcctc ttggacaagg
    44941 tttggcgaac gccgtcgggc tggcgatagc agagcgtacg ctggcggcgc agtttaatca
    45001 gccggatcat gaaattgtcg atcacttcac ctatgtgttt atgggcgacg gctgcctgat
    45061 ggaaggtatc tctcacgaag tctgttcgct ggcgggcacg ctggggctgg gcaagctgat
    45121 tggtttttac gatcacaacg gtatttccat cgacggtgaa accgaaggct ggtttaccga
    45181 cgatacggca aaacgttttg aagcctatca ctggcatgtg atccacgaga tcgacggtca
    45241 cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc
    45301 gctgattatc tgccgtacgg tgattggctt tggatcgccg aataaagcag gtaaagaaga
    45361 ggcgcacggc gcaccgctgg gtgaagaaga agtggcattg gcacggcaaa aactgggctg
    45421 gcaccatccg ccatttgaga tccctaaaga gatttatcac gcctgggatg cccgcgaaaa
    45481 aggcgaaaaa gcgcagcaga gctggaatga gaagtatgca gcctacaaaa aggcccatcc
    45541 gcaactggca gaagagttta cccgtcggat gagcggtggt ttaccgaagg actgggagaa
    45601 aacgactcag aaatatatca atgaattaca ggcaaatccg gcgaaaatcg ctacccgtaa
    45661 ggcttcgcaa aatacgctta acgcttacgg gccaatgctt cctgaattgc tcggcggttc
    45721 ggcggatctg gctcccagca acctcaccat ctggaaaggt tctgtgtcgc tgaaggagga
    45781 tccggcgggc aactacattc actacggggt gcgggaattt ggcatgaccg ctatcgccaa
    45841 tggcattgcg caccacggcg gcttcgtgcc gtataccgcg acgttcctga tgtttgttga
    45901 atacgctcgt aacgccgcgc ggatggcggc actgatgaaa gcgcggcaga ttatggttta
    45961 tacccacgac tcaattggct taggggaaga tggtccgacc caccaggccg ttgagcaact
    46021 ggccagtctg cgcttaacgc caaatttcag cacctggcga ccgtgcgatc aggtggaagc
    46081 ggcggttggc tggaagctgg cggttgagcg ccacaacgga ccgacggcac tgatcctctc
    46141 caggcagaat ctggcccagg tggaacgtac gccggatcag gttaaagaga ttgctcgtgg
    46201 cggttatgta ctgaaagaca gcggcggtaa gccagatatt attttgattg ccaccggttc
    46261 agagatggaa atcaccctgc aagcggcaga gaaattagca ggagaaggtc gcaatgtacg
    46321 cgtagtttcg ctgccctcga ccgatatttt cgacgcccag gatgaggaat atcgggagtc
    46381 ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa gcaggcatcg ccgattactg
    46441 gtacaaatat gttggtctga aaggggcaat tgtcgggatg accggctatg gggaatctgc
    46501 tccggcagat aagctgttcc cgttctttgg ctttaccgcc gagaatattg tggcaaaagc
    46561 gcataaggtg ctgggagtga aaggtgcctg atggtgatcg ccggatgcta tatgttcccc
    46621 tcaccctaac cctctcccca gaggggcgag gggacggtcc gtgcgctttt taggggcgca
    46681 tctgggattt tgtttaacgg gtgatccaca acgtgggcca ggcgtctggc ccatgccagt
    46741 tatcgcaggt gggttctgcg gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc
    46801 agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc accagttcac
    46861 gcgatttttc atcgaatgtt gcgttcatca gttccagttc attcgtctcc tgaccgctgt
    46921 tgaacggcaa acgtaaccga accgggcgcg aggccagtgg ctttttacgc gacacaatcc
    46981 atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc agcgctttat
    47041 catcggtcag cgcagtgaca ttcacctctc gacgcaatgg atcaagcgag cagcgcagac
    47101 cattcatccg ccagtttcca taatccagca aatcgttgcg ttcttcgagt gagagtggcg
    47161 tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt acgctgagcg
    47221 gcggttcatc ccctttcttt atccacgcgg tttcactgcc aacgcgcttt tgctgagcat
    47281 ctataaacaa caacgccgct tttaagccac tcagagaaat agtctgattg ccgtcgcgta
    47341 aggtgatcgc cctcccttcc tgaatcatct gcaaaaacgc ggtgatggtt gccgtatcat
    47401 cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaacgcc aacggctcac
    47461 catctaacag cagccgtggc gctatctccc cttcagacgc atccggcgac ttcaacccgc
    47521 cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc gctgcggctc agggtcatca
    47581 ccagtccgtt atgatcgccc gtattacgcg ccacgcagaa gttttggtta ttacaggtga
    47641 cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac gtcggcaaca
    47701 aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc ccagaagttc
    47761 aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat cggatttatc
    47821 ggattgagcc aataataaac aacgaaatat tattgttatt tttcagtcca ttaaatgtga
    47881 catttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc cagttttgat
    47941 catctccagc gccttgttga acggcagctc gagcacttca atatcttcat cttcgacacc
    48001 accgccggcg ttagcgcgct ggctgtcact gtattcggcg ataaagaagt ggattagctc
    48061 agtcacaccg cctggcgaca tatactgttc aaataatttg cgcacttcgc caacttcata
    48121 acccgtctct tcaatcgctt ctttacgaat acacacttcc ggttcgtcgt tatccagcag
    48181 cccggcgcag gtttcaatca gttgcccgct ttcattgcca ttaacccagg tagcgacgcg
    48241 gaactggcga atcagaacca cggtcttttt cttcgcgttg tacaggagga tcgttgcacc
    48301 attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt tgcgcgtgag
    48361 atcgtaagta atgttgtgca gggtgaaata gttatcggag agaattttgt ctttaatgag
    48421 ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattatc ttacgctgtg
    48481 aagccgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc cccagccagt
    48541 caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc agatccgcac
    48601 cacgcacggc gtcaccaccg gcgaagattt tcggattggt ggtctggtaa cggtactggc
    48661 tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc gactccagcc
    48721 acggcatccc gtgcggattg aagccaaacg ccataatcac cgcatcggct ggcatgacaa
    48781 attcactgcc ttccaccggc actggacgcc gacgcccctg ggcatccggc tcgccaagac
    48841 gcgtgcgcag gaaacgaatc ccgcagacgt gaccttgttc attcagttca agcgccaccg
    48901 gctggacgtt aaattcgaag ttggccccct cttcacgggc gttcttcacc tctttcttcg
    48961 agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc gcgccgtggc
    49021 gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa
    49081 gtccggcagt attgataaac ggctcttccg gtagctcttc gagtcccatc acctgtttag
    49141 tattggcgat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac
    49201 ccgctttcat ggagcggtaa gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa
    49261 gcgaattcaa agagacatct ttacccactt cacaattgag ttcgaagtga atccccatcg
    49321 cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa gaaggaatgc
    49381 cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc tctacgccat
    49441 tgcgggtcaa aacatccgca caggccagcc ctgccggacc cgcaccgata atcgccaccc
    49501 gcttgtccac tttggtgaca tggcttaagt caggacgcca acctttcgcc aacgcctgat
    49561 ctgaaatgta gcgttcaatg ttaccgatag ttactgcgcc gtgctcatcg cgaatagtac
    49621 aggcaccttc acacaaacgg tcttgcgggc aaacgcgtcc ggtaatttcc ggcagggtgt
    49681 tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc agttcaatcc
    49741 actgcggtat atggttatgc agcgggcagg tccattcaca aacgctgtgc tcgccgcact
    49801 taaggcagcg cgaggcttcc cgttgtacct gatcggcacg gaatggcaga taaatttcat
    49861 caaaaccggt tttgcgcgct tcaatcgcca gtttatctgg ctcgccacgc gcgggcgttg
    49921 cctgcatttg ctcgacttta ctcattaccg gcatttcttg cgccgcggta ctggcatgcc
    49981 acggttgatg ttcctgacgt gcggtacgca agcggcggga tttcgccatg cctgagagtg
    50041 cggcgtcagt gaccagttgc agcgcgtccg ctgggcaatt ctcgacacaa gcaggaccgt
    50101 tttcgcgccc tgcacaaagg tcgcatttat gcgccgtggc ttttactttt cctgccgcga
    50161 cgggggtcag gacgatttgc atcgtaccaa atggacaggc caccacgcag gatttacagc
    50221 caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt gcgccattag
    50281 ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcacggca ctacgttgct
    50341 gctgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg acatgttgct
    50401 catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga cattgctgac
    50461 tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa aaccttattc
    50521 tttatatgag tgttgttatt acccgactta caggggaatc ggcaatgttc atgtgcccag
    50581 aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca ctgactaaat
    50641 tgcgtttcag cgaattggaa cattaatgat tttttgtgga gaagacgcgt gtgattgtta
    50701 aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg ctgtcgattc
    50761 tttccacggg tatcgctctg ctaacgctgg cgagcagttt acgcgacgct gaggctatca
    50821 atattgccgg atcgctgcgt atgcagagtt accgcctggg ctacgacttg caaagtggca
    50881 gtccacaact caatgcacat cgccagttat ttcagcaggc actgcattca ccggtattaa
    50941 ccaatctcaa cgtctggtat gtgccagagg cagtaaaaac ccgctatgcg catctgaatg
    51001 ccaactggct ggagatgaat aatcggctca gcaagggcga cttgccgtgg tatcaggcca
    51061 atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag cactacgccg
    51121 aacgcaaaat gctgctggtg gtggcgatct ccctggctgg cggcatcggt attttcacgc
    51181 tggtcttttt tactttgcgc cgcatacgcc atcaggtggt tgccccgctg aatcagctgg
    51241 ttaccgccag tcagcgtatt gaacacgggc agttcgactc gccgccgctg gataccagcc
    51301 tgccgaatga gcttggcctg cttgcaaaaa cctttaacca gatgtcgagc gagctgcata
    51361 aattgtaccg ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc cacgaggcca
    51421 agcgtcgtct ggaggtgttg tatcagtgtt cgcaggcgct aaacaccagc cagattgatg
    51481 tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcggct gaatatctgg
    51541 agttaaatgt cggtgacaac tggcggatta gcgaagggca gccaaacccg gaattgccga
    51601 tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac tggcaaaata
    51661 gtcacgtttc atcatcagaa ccgctgctta acagtgtttc gtcgatgctg ggacgcggtt
    51721 tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa gaacgtgcga
    51781 ccatcgcccg cgaattgcac gactcgctgg ctcaggtact ttcttactta cgtatccagt
    51841 tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt atcatggccg
    51901 atttctccca ggcgctgaat gatgcttatc ggcagttacg cgagctgtta actactttcc
    51961 gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg gatacgttac
    52021 aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct gccaaccctg gcgctggatg
    52081 cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc
    52141 acgccaacgc cagcgaaatc gccgttagct gcgtcaccgc gccggatggc aatcacacag
    52201 tctatattcg cgacaacggg attggcatcg gtgaaccgaa agaacccgaa ggccattatg
    52261 gtctgaatat catgcgcgaa cgcgcggaaa gattgggtgg aacgctgact ttttcacaac
    52321 cttccggcgg cggcacgtta gtgagtatta gctttcgctc tgcggagggt gaggaaagtc
    52381 agctgatgta atgcctctta ttgaccaaag aatagtggca cttaaggttc agtataaaag
    52441 ggcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac cttgccgcta
    52501 cagtgaagca agtcaagcct acaacgatac gcagaaacac gaggtcctct tttaatggcg
    52561 aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt gtgtctgaca
    52621 ggtaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc gccaccgaat
    52681 gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa caccgtgacc
    52741 caggttatcg agcaaaatat gaccggcctc gataatctca tgtatatgtc gtctcagagc
    52801 agtggcaccg gccaggcatc tgtcacttta agttttaaag caggcaccga tccggacgaa
    52861 gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg
    52921 caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca atattctgac cattgccttc
    52981 gtctctaccg atggttcgat ggataaacag gatattgccg attacgttgc cagtaatatt
    53041 caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat
    53101 tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac ggctaaagat
    53161 gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact tggtggtacg
    53221 ccttccgtcg ataagcaggc gctcaacgcc accattaacg cccagtcact gctgcaaaca
    53281 ccagaacagt tccgcgatat caccttgcgg gtgaatcagg acggttcaga ggtaaggctg
    53341 ggcgatgtcg ccaccgtcga aatgggggcg gaaaaatacg attatcttag ccgcttcaat
    53401 ggtaagccag cctccgggct gggggtaaaa ctggcctccg gcgcgaacga aatggcgact
    53461 gcgcagctgg tgctcaatcg tctcgacgag ctggcgcagt attttccaca cggactggaa
    53521 tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa
    53581 acgctgctgg aagctatcgc tctggttttc ctcgttatgt atctgttcct gcaaaacttc
    53641 cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac cttctccgta
    53701 ctttatgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt gctggcgatc
    53761 ggtctgctgg tggatgatgc catcgtggtg gtggaaaacg tcgaacgtat tatgagtgag
    53821 gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca gggggcactg
    53881 gtcgggattg cgatggtgct ttcggcggta tttgtgccaa tggccttctt cggcggcacc
    53941 accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt gctgtcagta
    54001 ctggtagcga tgatcctcac tccggctctg tgtgccacgc tacttaagcc actgaaaaaa
    54061 ggtgagcatc acgggcaaaa aggctttttt gcctggttta accagatgtt taaccgcaac
    54121 gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg ctggattgtg
    54181 atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc
    54241 ttgccacttg aagaccgtgg catgtttact acctcggtac agttgcccag cggttcaacc
    54301 caacaacaga ccctgaaagt cgttgagcaa atcgagaaat actacttcac ccatgaaaaa
    54361 gacaacatca tgtcggtgtt tgccactgtt ggttctggcc ctgggggtaa cggacaaaac
    54421 gtggcgagaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa gaccggcacc
    54481 tcgtttgcca ttatcgagcg tgcaacgaag gcgtttaaca agattaaaga agctcgcgtt
    54541 atcgccagca gcccgccagc aattagcggt cttggtagtt ctgcaggttt tgatatggag
    54601 ttgcaggacc acgctggagc gggtcacgat gcgctgatgg cagcacgtaa ccagttgctg
    54661 gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct cgacgacagt
    54721 ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt tgctatcgac
    54781 gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga ctttatggat
    54841 cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat
    54901 gacatcaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt ctctgctttc
    54961 gcgacctcac gctgggagac aggctcgccg cgtctggaac gctataacgg ttattctgcg
    55021 gtagagattg taggggaagc cgcaccgggg gtcagtaccg gtacggcgat ggatattatg
    55081 gaatcgttag tgaagcagtt gccaaacggc tttggtctgg agtggacggc gatgtcgtat
    55141 caggagcggc tttccggcgc gcaggctccg gcgctgtacg ccatttcctt gctggtggta
    55201 ttcctgtgtc ttgcggcgtt gtatgaaagc tggtcggtgc cgttctcggt aatgctggtc
    55261 gtgccgctgg gggttatcgg cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac
    55321 gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa cgccatcctg
    55381 atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga agcgacgctc
    55441 cacgcctgcc gtcagcgttt acgcccgatt ctgatgactt cgctggcatt tatcttcggc
    55501 gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact
    55561 ggcgtaatgg gcgggatgat ttcggccact attctggcta tttacttcgt gccgctgttc
    55621 tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc aataaaaaag
    55681 gcgacatgct aatgtgtcgc ctttttcaac tttccgatta agaacctgct cagcgggttc
    55741 ttgctgtttg tactttgtct caggaattac ttacgaagca taacttcgat aaagtctttc
    55801 cagttcccca gttcacgttc aatcataaca acctctctta taattatggg tattctacgg
    55861 aaacaatata ccgtggtga
//
LOCUS       DAZGAP010000006        51967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGAP010000006 DAZGAP010000000
VERSION     DAZGAP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.006"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..335
                     /locus_tag="ACHLAM_000306"
     CDS             <1..335
                     /locus_tag="ACHLAM_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7851897.1"
                     /translation="KKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKPFS
                     TIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSNIDLAKRRYKEL
                     VQERKNEN"
     gene            325..615
                     /locus_tag="ACHLAM_000307"
     CDS             325..615
                     /locus_tag="ACHLAM_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7851898.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(668..2521)
                     /gene="asmA"
                     /locus_tag="ACHLAM_000308"
     CDS             complement(668..2521)
                     /gene="asmA"
                     /locus_tag="ACHLAM_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO7851899.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLKETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(2543..3124)
                     /gene="dcd"
                     /locus_tag="ACHLAM_000309"
     CDS             complement(2543..3124)
                     /gene="dcd"
                     /locus_tag="ACHLAM_000309"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO7851900.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(3216..3857)
                     /gene="udk"
                     /locus_tag="ACHLAM_000310"
     CDS             complement(3216..3857)
                     /gene="udk"
                     /locus_tag="ACHLAM_000310"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO7851901.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            4175..7492
                     /gene="dgcE"
                     /locus_tag="ACHLAM_000311"
     CDS             4175..7492
                     /gene="dgcE"
                     /locus_tag="ACHLAM_000311"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7851902.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(7601..8449)
                     /gene="alkA"
                     /locus_tag="ACHLAM_000312"
     CDS             complement(7601..8449)
                     /gene="alkA"
                     /locus_tag="ACHLAM_000312"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO7851903.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPLRSYALLHIWYTEG
                     WQPDEA"
     gene            8583..9935
                     /gene="yegD"
                     /locus_tag="ACHLAM_000313"
     CDS             8583..9935
                     /gene="yegD"
                     /locus_tag="ACHLAM_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7851904.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(9948..11887)
                     /gene="yegI"
                     /locus_tag="ACHLAM_000314"
                     /pseudo
     CDS             complement(9948..11887)
                     /gene="yegI"
                     /locus_tag="ACHLAM_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(11884..12645)
                     /gene="pphC"
                     /locus_tag="ACHLAM_000315"
     CDS             complement(11884..12645)
                     /gene="pphC"
                     /locus_tag="ACHLAM_000315"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO7851905.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWLP"
     gene            complement(12642..13301)
                     /gene="yegL"
                     /locus_tag="ACHLAM_000316"
     CDS             complement(12642..13301)
                     /gene="yegL"
                     /locus_tag="ACHLAM_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7851906.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(13522..13581)
                     /gene="ibsA"
                     /locus_tag="ACHLAM_000317"
     CDS             complement(13522..13581)
                     /gene="ibsA"
                     /locus_tag="ACHLAM_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO7851907.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(13854..13910)
                     /locus_tag="ACHLAM_000318"
     CDS             complement(13854..13910)
                     /locus_tag="ACHLAM_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7851908.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(14182..14238)
                     /locus_tag="ACHLAM_000319"
     CDS             complement(14182..14238)
                     /locus_tag="ACHLAM_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7851909.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(14510..14566)
                     /locus_tag="ACHLAM_000320"
     CDS             complement(14510..14566)
                     /locus_tag="ACHLAM_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7851910.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            14845..16092
                     /gene="mdtA"
                     /locus_tag="ACHLAM_000321"
     CDS             14845..16092
                     /gene="mdtA"
                     /locus_tag="ACHLAM_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO7851911.1"
                     /translation="MKSSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16092..19214
                     /gene="mdtB"
                     /locus_tag="ACHLAM_000322"
     CDS             16092..19214
                     /gene="mdtB"
                     /locus_tag="ACHLAM_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO7851912.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19215..22292
                     /gene="mdtC"
                     /locus_tag="ACHLAM_000323"
     CDS             19215..22292
                     /gene="mdtC"
                     /locus_tag="ACHLAM_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO7851913.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRSAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGALRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            22293..23708
                     /gene="mdtD"
                     /locus_tag="ACHLAM_000324"
     CDS             22293..23708
                     /gene="mdtD"
                     /locus_tag="ACHLAM_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7851914.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRCFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNCALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            23705..25108
                     /gene="baeS"
                     /locus_tag="ACHLAM_000325"
     CDS             23705..25108
                     /gene="baeS"
                     /locus_tag="ACHLAM_000325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO7851915.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            25105..25827
                     /gene="baeR"
                     /locus_tag="ACHLAM_000326"
     CDS             25105..25827
                     /gene="baeR"
                     /locus_tag="ACHLAM_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO7851916.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLMIDEGRFQASWRGKMID
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLEALDEE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26018..26350
                     /gene="yegP"
                     /locus_tag="ACHLAM_000327"
     CDS             26018..26350
                     /gene="yegP"
                     /locus_tag="ACHLAM_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO7851917.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            26498..27859
                     /gene="yegQ"
                     /locus_tag="ACHLAM_000328"
     CDS             26498..27859
                     /gene="yegQ"
                     /locus_tag="ACHLAM_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO7851918.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTTFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28132..28350)
                     /gene="ogrK"
                     /locus_tag="ACHLAM_000329"
     CDS             complement(28132..28350)
                     /gene="ogrK"
                     /locus_tag="ACHLAM_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO7851919.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(28432..29595)
                     /locus_tag="ACHLAM_000330"
     CDS             complement(28432..29595)
                     /locus_tag="ACHLAM_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO7851920.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDISGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(29595..30074)
                     /locus_tag="ACHLAM_000331"
     CDS             complement(29595..30074)
                     /locus_tag="ACHLAM_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7851921.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            complement(30089..32536)
                     /locus_tag="ACHLAM_000332"
     CDS             complement(30089..32536)
                     /locus_tag="ACHLAM_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7851922.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAETMAGVKLLMSGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTVASTGKLDKLTAAFKASDGKTSELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALVLTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFTNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(32529..32684)
                     /locus_tag="ACHLAM_000333"
     CDS             complement(32529..32684)
                     /locus_tag="ACHLAM_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO7851923.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGSTNE"
     gene            complement(32681..32956)
                     /locus_tag="ACHLAM_000334"
     CDS             complement(32681..32956)
                     /locus_tag="ACHLAM_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO7851924.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(33013..33531)
                     /locus_tag="ACHLAM_000335"
     CDS             complement(33013..33531)
                     /locus_tag="ACHLAM_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO7851925.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(33544..34734)
                     /locus_tag="ACHLAM_000336"
     CDS             complement(33544..34734)
                     /locus_tag="ACHLAM_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO7851926.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            35147..35590
                     /locus_tag="ACHLAM_000337"
     CDS             35147..35590
                     /locus_tag="ACHLAM_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7851927.1"
                     /translation="MLDIRMSRPSEASEIIQIWKSSVDATHDFLTGHDRQEIEKEVIG
                     FFSETPVWVATNQDDQPLGFMFLHEGHLEALFVAGSARGLGVGKRLISHALALHPELS
                     VDVNEQNQQAVGFYQHMGFQVSGRSDLDNQGRPYPLLHLRMANNM"
     gene            complement(35611..36195)
                     /locus_tag="ACHLAM_000338"
     CDS             complement(35611..36195)
                     /locus_tag="ACHLAM_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7851928.1"
                     /translation="MDKKFKNQIVDSDIARLLLKHRKARNLTVTELAQRSGVSQAMIS
                     KIERGISSPSATILSRLANAMNVTLSKLFAELEQHQNSLVRFAHQQQHWTDEQSGITR
                     WSLSPAGACPELIKVEIPAMGELTIPASANEHLSGQTLWMMSGSLDFRVKNQTHHLRA
                     GDCLALTFASEYQMSNPDSEQSCSYIVAFSQKNQ"
     gene            complement(36431..36958)
                     /locus_tag="ACHLAM_000339"
     CDS             complement(36431..36958)
                     /locus_tag="ACHLAM_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7851929.1"
                     /translation="MQHLKNITAGNPKTVAQYQLTKNFDVIWLWSEEGKNWYEEVSNF
                     QEDTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVI
                     KRIYTADEQEQQAESQKAALLSEAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(36962..39007)
                     /locus_tag="ACHLAM_000340"
     CDS             complement(36962..39007)
                     /locus_tag="ACHLAM_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7851930.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSMSETLAATPKAVKTVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQ
                     IANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTA
                     LAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAV
                     QKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNGNEYFKW
                     RSRQSTTTKDLMNLKWDALYVLVKALFSSEVKISTVNALRIFNSSFGAIFRRSEENLY
                     IIPTRENEGENGDIGSLRPFGINLRTGVVSVGNGARIDGGLALGTNNALGGNSIVLGD
                     NDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDI
                     RLGTRVVQTMQKGVMYEKAGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
     gene            complement(39018..39548)
                     /locus_tag="ACHLAM_000341"
     CDS             complement(39018..39548)
                     /locus_tag="ACHLAM_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO7851931.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDSCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWETSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(39541..40449)
                     /locus_tag="ACHLAM_000342"
     CDS             complement(39541..40449)
                     /locus_tag="ACHLAM_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO7851932.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELLWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(40454..40801)
                     /locus_tag="ACHLAM_000343"
     CDS             complement(40454..40801)
                     /locus_tag="ACHLAM_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO7851933.1"
                     /translation="MTARYLGMNRSNGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(40798..41433)
                     /locus_tag="ACHLAM_000344"
     CDS             complement(40798..41433)
                     /locus_tag="ACHLAM_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO7851934.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITSTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            41517..42302
                     /locus_tag="ACHLAM_000345"
     CDS             41517..42302
                     /locus_tag="ACHLAM_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851935.1"
                     /translation="MFSDNVINAWWFISLYLFLLIALTFVTFGKSNLMRFIAHHFNLE
                     YSDRKLKMLDKKWRDIQLFKTINGINVSGIEDVRMIQQGLIDGKLKTSYFFLTRFWGD
                     ITKPPHIIKTIIVILASIIYILFACYIHNKQSAIVRDAIGIPYKNMMYYVYSDKVLLS
                     FKNKTVELNKTYSLADCKTLQNVFIKDTLPEIACNKLLQLNEEDSEWLSQEIKDNNSH
                     KKALLILSLVYFISGLLIFLSYTKFLYANKKVLEYKASNKNHS"
     gene            complement(42374..42826)
                     /locus_tag="ACHLAM_000346"
     CDS             complement(42374..42826)
                     /locus_tag="ACHLAM_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO7851936.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSTELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEETILAHLER"
     gene            complement(42819..43286)
                     /locus_tag="ACHLAM_000347"
     CDS             complement(42819..43286)
                     /locus_tag="ACHLAM_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7851937.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSMSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(43249..43494)
                     /gene="lysC"
                     /locus_tag="ACHLAM_000348"
     CDS             complement(43249..43494)
                     /gene="lysC"
                     /locus_tag="ACHLAM_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO7851938.1"
                     /translation="MLCAGCTSARPAQTPVIVHNACPKVSLCPMPGSDPQKNGDLSAD
                     IRLLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(43394..43819)
                     /gene="lysB"
                     /locus_tag="ACHLAM_000349"
     CDS             complement(43394..43819)
                     /gene="lysB"
                     /locus_tag="ACHLAM_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO7851939.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVAITRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            complement(43807..44232)
                     /locus_tag="ACHLAM_000350"
     CDS             complement(43807..44232)
                     /locus_tag="ACHLAM_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO7851940.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVATWAILATVIC
                     VVAGGVGVYATEYVLELYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRKACRQRLEGRELCQS"
     gene            complement(44247..44744)
                     /locus_tag="ACHLAM_000351"
     CDS             complement(44247..44744)
                     /locus_tag="ACHLAM_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO7851941.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(44744..45025)
                     /locus_tag="ACHLAM_000352"
     CDS             complement(44744..45025)
                     /locus_tag="ACHLAM_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7851942.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(45029..45232)
                     /locus_tag="ACHLAM_000353"
     CDS             complement(45029..45232)
                     /locus_tag="ACHLAM_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO7851943.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(45232..45741)
                     /locus_tag="ACHLAM_000354"
     CDS             complement(45232..45741)
                     /locus_tag="ACHLAM_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO7851944.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(45841..46584)
                     /locus_tag="ACHLAM_000355"
     CDS             complement(45841..46584)
                     /locus_tag="ACHLAM_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO7851945.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDIRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRVPRKTRSVTPAKRGRPKKKAS"
     gene            complement(46588..47661)
                     /locus_tag="ACHLAM_000356"
     CDS             complement(46588..47661)
                     /locus_tag="ACHLAM_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO7851946.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(47720..48574)
                     /locus_tag="ACHLAM_000357"
     CDS             complement(47720..48574)
                     /locus_tag="ACHLAM_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO7851947.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHDAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            48748..50520
                     /locus_tag="ACHLAM_000358"
     CDS             48748..50520
                     /locus_tag="ACHLAM_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO7851948.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            50520..51554
                     /locus_tag="ACHLAM_000359"
     CDS             50520..51554
                     /locus_tag="ACHLAM_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7851949.1"
                     /translation="MSKKKGKTPRPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDND"
BASE COUNT        11798 a        14248 c        13542 g        12379 t
ORIGIN      
        1 gaaaaaagct ggcttttctt gataccagtc ttgacgattt acgcgcattt ccagaatcgt
       61 ctcgacagga aattggctat caactcgaca ggatccaaca agggttaaac ccatacgact
      121 ggaaaccttt ttcaactatc ggccctggtg tgcgagagat tcgtactcgc gatgctgatg
      181 ggatctatcg cgtcatgtat gtcgcaaaat ttgaagaagc ggtttacgta ttgcattgtt
      241 ttcaaaagaa aacacagact accagtcaat cgaatattga tttagccaaa cgacgctata
      301 aagaacttgt tcaggagcgg aaaaatgaaa attgaaacct ttgatagtgt gtgggatgcg
      361 gtgagtgaca cccctgaaca agcggaaaat atgcggatcc gtgccgaact tgttacgatc
      421 attaataact ggattgaaca acaagggttt agccaggctc aggcagcgtc tgctttaggg
      481 gtcactcaac ctcgcatatc tgaacttgcc agaggtaaga tccagatctt tagtatcgat
      541 aaattgatca ccatgatggc tcatgcagga ttgcatattc aacgtattga agttcaatat
      601 ccacatgctg cttagtacta taaatacaga tggcgacaaa ttgacgccgc catctgcata
      661 acttgtctta catcttctcc agcaacttct tcacatcttt gccattgcgg gaatctttat
      721 tccgctcggc ccagtcattc aggcgacgtt tcgcttcgtc ctgtagatgt ttgcgcagta
      781 actgatccac ttgcaggctg taattaagtt gctgccagtt gccataaacc cgcagcggta
      841 ctggcgtttc tttgaggaaa tcaatcagtt tgctttcccc gttccaaccg ccaatcacac
      901 gaatgtcaaa ctgggtgtcg caggtttgat ctgccagatt caacatgcct tcccctgtca
      961 gcgccagcac aggcgattga ccttgcatgt cgtttaacgt cacgacgcca tccttcaacg
     1021 tcaaatcggt ggtaaagcgg tcaagacgcg ttacgttatc gaagttttca gcggccttca
     1081 catcaccacc attacgttct accgcttgct gaatcatctg ctggaagttc atcccttcca
     1141 tgcgcgtgtc ggtcatttcg acatgtgcct gtccttgcca gttatggcgg aatgcgtcgg
     1201 catctatgtc agcaccggag aaatcaccag ccagtgacat ctttccggtc aacgaaatcg
     1261 gatagttaaa cgccttcaga atggtgccaa tctcaacgtt ttccagccgt ggctggaagt
     1321 ttattcgcgg atttattgat gtcgcgtcca gcgtgcccgg cagtgaaacc tgtccaccgt
     1381 taagtttgcc ctgcagttga gtaatttcca gcagacccga cttgttggtc atttgcgtgg
     1441 caacatctgt aaaattcatt ccgcgccagc gcacgttact ggcctgcaac aaaatatcag
     1501 ccgtaaagcc ttgcagtccc tgataggccg gttcatcaat acgcgaagaa atgaccgggc
     1561 gcggcaacgt ggattggctc tgcccctgct gcgcggcacc gttttcaccg ttcgctgttt
     1621 ctttaagcgg gatgaggttg tcaagattca gttgcgggaa ttgcagcctc agctgccatt
     1681 ccggtttctc tgtcagcgtg acctgtgctt gcccgctcag cgtactgtca ttggcggtca
     1741 agctgatttg gttaaatgaa aggcgtttat gtgactcctg ccactgggcc tggaaactcc
     1801 cctgcccctg aataccttgt tttggtaaat ctgcaccctg caactgccag ttaacttgtt
     1861 caatagccgc cgttaaatca tgcggataat cagaagcatc taccgtaccg ttaagggata
     1921 tcgtgagatc gcgctgatcg cgattaaccc gcccggagaa ctcaaatgaa ccacgatgtt
     1981 ggggatcttg ttccatttgc aggcgaatat tgcggattgt cacctgctcg tcatcttcat
     2041 gctggaacac cagcacgctg tccgccacct taagactgga tatatcaaac gaccatccgc
     2101 gatcgtctga cagatccggc aaggtattgt cgcgcggtgc aaccggagcg tcttcactgc
     2161 gcaccgcttc cgtctgcggc gtcagttgga tcactgcccc ttttagcatc acctgcttaa
     2221 cgctcagttg atgactcagc agtggtaaaa gcgccacgtc cagacgcatg ttgtctgcgc
     2281 gaaccagtgg ctggctcgcg ccctgggcag tgagagacat tcgcccggag aggatactaa
     2341 gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg caattgataa ccgctacgtg
     2401 cagcaacttg cttgaccata tagtcgcgga aatcattcgg attcaccagc aacactaacg
     2461 cagataaccc ggccaccagc acgaccagga gtatcatcag cgtcgtcaga aatcgtctca
     2521 tggtatcctc aatgggcctg aattagtctt tatcgattcg actggctacc gcgccctgct
     2581 ggttgcgata tttcgcatct tcacggcggt tgtaaggtcg cgccgccggg ccggaaagcg
     2641 gctcaaagct cagcgcacca attaacatgc ccggacgcag cgccagcggc agcttaccgg
     2701 agttgtagaa ctccagcaca atacagccag accagcccgg atcgatacgg tgcgcggtga
     2761 cgtgcaccat aagccccaga cgcgccagtg aggaacgacc gtccagccat cccaccagat
     2821 cggctggcag cgtcaccgac tccagcgtca ccgccagcgc cagctctcct gggtgaagat
     2881 aaaacgcctc gctttcatcg agtacgatct catcgctcat cacgcggtca agcgcggcgc
     2941 tcacttcatc tttgggaccg ctcagatcga taaacgcagc cgtgtgacca cggaaggtac
     3001 gaaatttatt gcccaggcgc acatccaccg tcgcgccgtt aatacgctcc actggcggac
     3061 gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc aatatctcgg tcacacagac
     3121 gcatggcatt ttctcctttc gcgcatcact cccttaacgc caattacgtc aagggcatac
     3181 caggttatca ctgaacggta cacaatttat caagcttatt caaagaactg acttattttc
     3241 gctttcaata tatcgatggc gatgcggttt ttcccgccgc gcggcacgat aatgtccgca
     3301 tattgtttag aaggctcaat gaattgcagg aacatcggac gcacggtttt ttgatattgc
     3361 gccatcactg aatccattga acgcccacgc tcgttaacgt cacgcttgat gcggcgcatc
     3421 aggcagatat ccagcggggt atcaacgaaa atggagaagt taagttcatc acgcaaacgc
     3481 gcatccgtca gcagcaaaat gccttcgaga atgatgactt tcttcggctc aaccgtcacc
     3541 gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg gcaggtcaat ggccgagccg
     3601 cgtttcaacg cttgtaaatg ctcaagcaac aggctgtgat ccatagcgct gggatggtcg
     3661 tagttggtct taacgcgttc ttccatcgac agatggcttt gatctttgta atagcagtct
     3721 tcgggaatta cgccgatgtg ttcatcaccg acttgctcac gtaattcacg ataaagggta
     3781 ctggcaataa gactcttgcc ggaagccgat gcgccagcga taccgataat gacgcactga
     3841 tgagactgat cagtcatata tttagcgacc tgattaacct ggatgttagg aagggggcga
     3901 cgaagcgcca aacgcggcaa ttatagggat ttcatccgcc tgataccagt cgaatagcgt
     3961 tgccgcgcgc tcagagttaa ttgttgacga agaattcccg gtggcaaatt acgttgatca
     4021 gttttataca aggtaaaaat tgttatacgc agttgcgcaa attatccgcc tttacgtcac
     4081 tttatgagca attcgcatat aaaatgtaaa acttttgtac tagcataaac acagaaacga
     4141 atactggcga cctggtcttg cggataaagc ggtaatgagc aaacaatcac agcatgtatt
     4201 aattgccctg ccccacccgc tgcttcacct ggtcagttta ggtttagtct cgtttatctt
     4261 tacccttttc tcgcttgagc tttcgcagtt tggcacccaa ctcgccccac tgtggttccc
     4321 gacgtccatc atgatggtgg cgttttatcg ccatgccggg cgcatgtggc cggggattgc
     4381 gctgagctgc tcgctgggaa atatcgccgc atccatcctg cttttttcca ccagctcgct
     4441 gaacatgacc tggacgacca tcaatattgt tgaagccgtg gtcggggcag tgctactgcg
     4501 taaattgctg ccgtggtata acccattgca aaatctggct gactggctgc gtctggcact
     4561 cggcagcgcc attgttccac ctctgttagg gggtgttctg gttatcctgc tgacgcccgg
     4621 agacgatcct ctcagggcat ttttgatatg ggtactgtca gaatccatcg gcgcactggc
     4681 actggtgccg ctgggattgt tatttaaacc acactatctg ctgcgccatc gcaacccacg
     4741 gttgcttttt gagtcgctgc tcacgttagc catcacactg acgttaagct ggctttcgat
     4801 gctgtatctg ccgtggcctt ttactttcat tattgtgctg ttgatgtgga gcgccgtgcg
     4861 cctgccacga atggaagcct ttttgatctt ccttaccacg gtgatgatgg tgtcactgat
     4921 gatggccgcg gatccctccc tgcttgctac gccgcgtaca tacctgatga gccatatgcc
     4981 gtggctaccg tttttgctga tcctgctgcc cgccaacatc atgaccatgg tgatgtatgc
     5041 ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc cgctttcgga acgcgatgga
     5101 atattccgcc atcggcatgg cattagtggg caccgaggga caatggctgc aatccaacaa
     5161 agcgctctgc cagtttctcg gttacagtca ggaagagctg cgcggactca cctttcagca
     5221 actgacctgg ccggaggatc tcaataaaga tctgcaacag gttgaaaagc tgatcagcgg
     5281 tgaaataaac acctattcaa tggaaaaacg ctactacaac cgcaatggcg atgttgtctg
     5341 ggcgttgctt gccgtctcac tggtgcgcca caccgatggt acgccgctct attttatcgc
     5401 tcagattgaa gacattaacg agctaaaacg caccgaacag gtgaaccagc aactgatgga
     5461 gcgcatcacg ctggctaacg aagcgggcgg gattggcatc tgggagtggg agctaaagcc
     5521 gaatattttt agctgggata agcggatgtt cgagctgtat gaaattcctc cgcatatcaa
     5581 accgaactgg caggtgtggt acgagtgcgt actgccggaa gatcgccagc atgccgaaaa
     5641 agtgattcgt gattcgttgc aatcacgctc gccctttaag ctggaatttc gcattgccgt
     5701 gaaagatggc atccgccata tccgcgccct cgccaaccgg gtactgaata aagaaggcga
     5761 agtcgaacgc ctgctcggca ttaatatgga tatgaccgaa gtgaaacagc ttaacgaggc
     5821 attgtttcag gaaaaagagc gcctgcacat tacgctggat tccatcggcg aagccgtggt
     5881 ctgtattgat atggcaatga aaattacctt tatgaatccg gtggcggaga agatgagcgg
     5941 ctggacgcag gaagaagcgt taggtgttcc gctcctgacg gtgttgcata ttacttttgg
     6001 cgacaacgga ccattaatgg agaacattta cagtgccgac acctcacgtt ccgcgattga
     6061 acaagatgtg gtgttgcact gtcggagcgg cggcagctac gacgtgcatt acagtattac
     6121 gccgttaagt actctggacg gcagcaatat aggctcggtt ctggtgattc aggacgtcac
     6181 cgaatcgcgt aaaatgctgc gccagctgag ctacagcgcc tcccatgatg cactgacgca
     6241 tctcgccaat cgcgccagtt ttgagaagca actacgcatc ctgctgcaaa cggtaaacag
     6301 tacacatcag cgacatgcac tggtgtttat cgatcttgat cgctttaaag cggtgaatga
     6361 cagcgccggg cacgccgctg gcgacgcttt actgcgcgaa ctggcgtcat tgatgctgag
     6421 tatgctgcgc tccagcgacg tgctggcgcg actcggtggc gatgaatttg gtctgctgct
     6481 gccagattgt aatgtcgaaa gtgcgcgttt tatcgctaca cgcattatca gcgccgtgaa
     6541 tgactatcac tttatctggg aaggacgtgt acatcgggta ggtgccagtg ccgggattac
     6601 cttgattgat gacaacaatc atcaggcggc tgaagtgatg tcgcaggctg atatcgcctg
     6661 ttatgcctcc aaaaatggtg gacggggccg ggtgacggtt tacgaaccgc agcaagctgc
     6721 cgcacatagc gaacgggcgg tgatgtcgct tgatgaacag tggcggatga ttaaagagaa
     6781 tcagttgatg atgatcgccc acggtgtcgc ttcgccgcgg atcccggaag cgcgtaattt
     6841 gtggctgatt tcacttaagc tctggagttg cgaaggcgag attattgatg aacaaacatt
     6901 tcgtcgtagc ttcagcgatc cggcacttag ccatgctctt gaccgacggg tattccacga
     6961 ttttttccag caggccgcaa aagcgattgc cagtaaaggc ttaagcatcg ccctccccct
     7021 ttccgttgcc ggtttgagta gcgccacgct ggtgaatgaa ctgcttgagc agctggaaaa
     7081 tagccctcta cccccacggt tattacatct gattattccg gcagacgcga ttttagatca
     7141 cgcagaaagc gtgcaaaaac tgcggctggc gggatgtcgg atcgtattca gtcaggtggg
     7201 ccgcgatctg caaatcttca actcgctgaa agcgaatatg gcagattacc tgctacttga
     7261 tggtgagtta tgcgccaatg tgcagggaaa tttgatggat gagatgctga ttacgatcat
     7321 tcaggggcac gctcagcggc tcgggatgaa gaccatcgcc gggccagtag ttttacccct
     7381 ggtgatggat acgctttctg gcatcggcgt cgatctgatt tatggcgatg tgattgccga
     7441 tgcccaaccg ctggatttgc tggtgaatag cagttatttc gcgattaact gaggcgtgct
     7501 tctccatcgc ctgatacgac gctaacgcgt cttatcatgc ctacaaatcg ctcattcccc
     7561 aggccggata aggcgctcgc accgcatccg gcgaccaacg tcatgcttcg tctggttgcc
     7621 agccttccgt ataccagata tgcaacagcg cataagaacg caagggcttc cagcgttcgg
     7681 cataacggcg gatttgtgcc ggtgtcattc ccggaaatcg ctgtttaatc agataatcat
     7741 ccggcaggaa aacatctttc gcctgccagc cgcgcaaggc gaaataattc gccgtccagc
     7801 gcccgatacc cggaaaagtt tgcagcgttt tcatcgcctg ctccacatcg cccggtattg
     7861 tcattggtaa ggtgccctcc agcgccgcat ttgccagatg aatcagcgcc tctgcccgtt
     7921 tcaacggcat acctaacgct tttaatgcct gcggatctgc tgctgccagc cgctgaggcg
     7981 tggggaagca gacataatcc ggaaaatcat ccagcctttc gccataaagc tgtgccactc
     8041 tggcggtcaa ttttgccgcc atcgccacgc tcaccagctg gcctaaaatc gcccgcacgc
     8101 cctgctcaaa agcatcaaca caaccgggta aacgcaatcc gggccgtgcc gcgcctaact
     8161 tgcccaacgc accgttaaca atctgcgggt tacattgcag atcaaacagg cggctcattt
     8221 tcgccagaca ctctgcggca acgggttcta aacctgcact taaatttatg tgcagagtat
     8281 ggctggctat atccggaata gcagtcacca cgccgcgaca ttcgcccacc gccagactac
     8341 gggcataata atcgtcagcg accgtttcca caccgttcac cgcacgggcg gcgagaaatc
     8401 ccaacatcca cgaccagtca tacggcggct gccagttcag ggtatacatc gcatctcctt
     8461 gttaatccgc tttcagcata aacgttattc agacgctacg ctttgctttc atattccggt
     8521 tgtcgcgacg gcaacatttc gctaaagtca cgccccttct tcaccggcat ggggattatt
     8581 tcgtgtttat tggttttgat tacggtacag caaactgttc agtggcggtc atgcgcgacg
     8641 gtaagccgca attactgaaa atggaaaacg acagcacgct gctgccttca atgctttgcg
     8701 cgccaacgcg tgaagcggta agcgaatggc tgtaccgcca tcatgatgtt ccggcagacg
     8761 acgatgaaac gcaggcgctg ttgcgtcggg cgattcgtta taaccgcgaa gaagatatcg
     8821 atgttacggc gaaaagcgtg cagttcggtc tttcctcact ggcacagtac attgatgatc
     8881 cagaagaggt gtggtttgtg aaatcaccaa aatcattcct cggtgccagc ggcttaaaac
     8941 cgcagcaggt agcgctgttt gaggatctgg tctgcgcaat gatgttgcac attcgccagc
     9001 aggcgcaggc gcagctgccg gaggcaatta ctcaggcggt gattggtcgc ccgatcaact
     9061 tccaggggct gggcggcgat gaagcaaacg cccaggcgca agggattctg gaacgcgcgg
     9121 cgaagcgtgc cgggtttaag gatgtggtat tccagtacga gccggtcgcg gctgggctgg
     9181 attacgaagc caccttgcag gaagaaaaac gggtgctggt ggtggatatc ggcggtggta
     9241 cgactgactg ttcattgctg ctgatggggc cgcagtggcg ttcgcgtctc gatcgtgaag
     9301 ccagcctgct gggtcacagt ggttgccgta ttggcggtaa cgatctggat atcgcactgg
     9361 cgtttaaaaa cctgatgccg ttactgggca tgggtggcga aaccgaaaaa ggcatcgccc
     9421 tgccgattct gccgtggtgg aatgcggttg ccatcaacga cgttcctgcg caaagtgatt
     9481 tctacagtag tgccaacggt cgtctgctta acgacctggt acgcgatgcc cgcgaaccgg
     9541 aaaaagtggc cctgttacag aaagtctggc gtcagcgttt aagctatcgc ctggtacgta
     9601 gcgcagaaga gagcaaaatt gcgctttcaa gcgtagcgga aacccgcgcc tcactgccgt
     9661 ttatcagcga tgaactggct acgctgatta gccagcaagg gctggaaagc gccctcagcc
     9721 agccactggc gcggattctg gaacaggtgc aactggcgct ggataacgcc caggaaaaac
     9781 cggatgttat ctatctgacc ggcggtagcg cccgttcacc gctgattaaa aaagcgctga
     9841 cagaacagtt gccgggcatt ccgattgcag gtggcgatga ctttggctcc gtcaccgccg
     9901 ggctggcacg ctgggcggaa gtggtgtttc gttaattgaa aactggctca gcaacggctt
     9961 aaatcagcct gcgcctgagc cagtttttct gccgcctgac gtagcggttc catcaacggc
    10021 atggtccgtg ctggagcatg aaggcggaat cgctgcaact ctgtcgcacc gacattcagc
    10081 gccgattcca gccgatgtcg tttggcggcc atttttgcca ttaccgcttg tctgtctgca
    10141 ggcgttaccg cttcgttcgg cctgaacaca aaacggcgtt cacagctcgc tttccagtcg
    10201 ataaccgcct gggtcagatg atcaccaaaa cctttcactt gcttaacgct ccgtcgggta
    10261 acatccgctg ctgtttcaat accaaaggac cgtaacgccg ctttacgcgc agggccaacg
    10321 ccgggaatag aggcaacatc aataaaaaat ccctccagaa acttctgctt ctgccgttcc
    10381 cttgcggtgt cctgaagtgc tgccagatcg cgcttttctt cttccggtaa cccaagaatt
    10441 tcgtccttca ttttttcgag catcgtccgt ttggcgataa aaccttccag cccgcccaac
    10501 tgctggatct ggctgactaa atggtcatag tccattttcg cgcgattaaa cgcctcacgg
    10561 cgttgctgga tttctgcttt gtacgctttg cttgtcaggc tgccaataat ccagatagcc
    10621 gccagcacag gaaccaaaat aatataacgt ggttctgtct gaaggccgca aagcagcagc
    10681 gataacgctg acagtgcgat ctcaatcaga atgatgtatt cacgctttaa caggcctaaa
    10741 ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg gtaattgcaa tgctggcggt
    10801 gctactgacg ccatcaccat cgcccagact ttcgccagca caaaatcacc gctggtggta
    10861 atgacctctt cgccgagatc aataaaatag ataacgcctt gattatccag agcacaccac
    10921 gggcagtcag ccaaatgacc gggataaaca tgcattgccg aaacggtaca tttctttaac
    10981 tgttggcgta gtaaatcaag tgccgctacc cacgcttttg ccgtcggacg cccggttgcc
    11041 acgccacttt ccgtgaatgc ctgctgaaac atggcttcaa catgcccggt aacatcgaca
    11101 gcggaataga tcgtggcggc ggttttaaac cacgtcgctg attatctgac gcgtaggcat
    11161 aacggaaatg ggcaatatcc gtctccagtg cattacccgc atcagagaca agcggtacac
    11221 cggaataagg atgccgccca ccaaacaaga cgtgaaaaat cagcaacgca aggccaaaat
    11281 tatcgtgatt cgcggtgcgt tcaaagccga caaatgatgg caaggtttgt tgctctggcg
    11341 gcgtaaaatg cgacacgccg acttcgcata aatgcagcgt gccattggcg ttaatctgaa
    11401 aggagtcgct atcaatcaac accactttgc tgtcgcgacc taccataaag ctgttctggt
    11461 ttacgtcacc cacgacgtgc ccgtgctcgt gaatcgtagc aaaagatgaa gcaatattgc
    11521 gcgcaacata gagtagaaaa tcccacgcac aatgagggta acgttgacga cgatgtgccg
    11581 ggctatagat catatgaatc ggttctttac cagaaacttt tggcatcata aaaccgatta
    11641 cttttccgcc tcgtccaccg tgaagcgttg cctgcggcca ggcgacataa ttcaacaact
    11701 gcgcgtcagc tgtcgcagcc ataaaggcaa gtttgtcctg ttttaaggcg ggtggcggcg
    11761 tgtgataaat cttggcgacg ctatcgacaa actcctcgat atcataaacc gcgccttcac
    11821 cgcctttgcc cagttcacag cctggcgtaa cgcactcacc agtagcagta tataaagtgg
    11881 gtttcatggt aaccacagcg ccagtgccaa cgttttatca tcatcggtac gctcgtttac
    11941 cgccgggccg gacaaaaact gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt
    12001 tgctgacgcc agcccattga aaaacggggt aaagaaaggc acatgcggag aattatccag
    12061 catatttaac gccagccgct ggataccatc cgtaaatgct gcaactttgt gcgcacgctc
    12121 agtgctggtg aaagtttcca gtctggaaac ggcatcttca tcggtaatga agtgcgtcat
    12181 attggtatat tcacccacca tcggcgtgag cggcagttgc aaaccgtgcc ccaaatctac
    12241 caccacgccg ccatcgccaa tctgcataat caaggtgcca ttagctgatg aaatcaatcc
    12301 caaaaacgtg caggcaaaat cgcgaaccgc taattctttg gcttccgcct cggcaaaaag
    12361 tcgctgacga atggtcagca caatgtccgt tgcgagaaca tcattaagcc ccaattcccc
    12421 gccctgcact ttttgcgaca tataagccat cgcttcattg acggcgagca tcgcaccttc
    12481 gcctccctgc gaggcgctac ctgcgccatc agcgacaaat accgacaata atggctgctg
    12541 gtcatttaac caggcaacct gcatctggca ggcatcctgg caaggcaagt ccgcgctgat
    12601 gtgcgacgtt ccaacggttg atgcatagac cagacgccag ctcacactga cgtccagcct
    12661 tttggcgctt ccagcacaac ttccgttccc ggcgtggagc gggaaaccga acgcagcgaa
    12721 ctggataacc agctaaatag ttcgcggaat tgtaatcctt gaagaggcaa aggctgacga
    12781 acgctgattt gggccagcgt cttcatatcc gccccctgaa caccgatgga aaagaaggca
    12841 aacttcttat cttcttcccc ctgaaacacc ttgttggcag cagcctgcca ctcatcggtt
    12901 ggtgcgccat cggtaatcag gaaaatccac ggacgataat aggaaatacc attggcgcga
    12961 tactcacgtt tgcgctcctc aaccatatcc agggctttgg taatggcggc ccccattggt
    13021 gtatcgccct gggcaaacag gatgggcggg aaaaaattag cggcgctggt aaatggttgt
    13081 tccacatgca ccgggccgaa cgtcacaatg ccgagttcca ctctttttaa ggccagcgga
    13141 tcggcaagca gctcatcgcg aaaggtaacc aatccggcgt taagttcatt aataggtcta
    13201 ccgttcatag aaccagaaac atccaacagc aaaatgcagg ggcaacgtgg ttccgggtta
    13261 ctggcaaaat cgctggtggc aaacgtgatt tgttcgctca taagaaatcc tttcttagag
    13321 aattattgag gcccggtatc ataccgactg caacaatgaa ataagagcaa acgaagaaac
    13381 tttcagaatt aggcccggcg gcgcagaaaa ttttgctatt cgcctcgcaa gaacggagag
    13441 ccgtcgattg acactaaggg cggagtgaca taatttcagg agtgagggtt agggagaggt
    13501 ttccccctcc ccctggtgtt cttagtaagc ctggaagcta atcactaaga gtatcaccag
    13561 tatgatgacg tgcttcatca taaccctttc cttattaaaa gccctcttct ccgggagagg
    13621 gtttcccgtt tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc ctgaatgcag
    13681 tctatcgcta acgcgtgaac aaaatagttt cctctgtgat tattgcgtaa cgactcgcaa
    13741 agaatatttg ctggcaggat cgcagactac aaagcctgcg gattgacaat cttatcgtga
    13801 aggcataatt tcaggagtga gggtagagcg gggtttcccc cgccctggta gtcttagtaa
    13861 gctggccagc taataactaa gagcaccacg atgatgagta gcttcatcat gaccctttcc
    13921 ttatttaagg ccccttcctc gggaggggct ttcccgtttc agcgtcccgc tgaaatcggc
    13981 ggcctacctc ctttcgccat gcaagcagtc tatcgctaac ttgcagataa aatagtttca
    14041 catgtgatta ttgcgtaacg tctcgcaaag aatatttgct ggcaggatcg cagactacaa
    14101 agcctgcgga ttgacaatct aattgtgaag gcatactttc aggagtgagg gtagagcggg
    14161 gtttcccccg ccctggtagt cttagtaagc cgggaagctt atgactaaga gcaccacgat
    14221 gatgatccac ttcatcatga ccctttcctt atttaaggcc ccttcctcgg gaggggcttt
    14281 cccgtttcag cgtcccgctg aaatcatcgg cttacctcct ttcgccctga atgcagtcta
    14341 tcgctgactt gcggataaaa tagtttcaca tgtgattatt gcgtgacgtc tcgcaaagaa
    14401 tatttgctgg caggatcgca gactgcaaag cctgcggatt gacaatcgga ccgtgaaggc
    14461 atactttcag gagtgagggt agagcggggt ttcccccgcc ctggtagtct tagtaagctg
    14521 ggtagctaac gactaagagc accacgatga tgagtatctt catcatgacc ctttccttat
    14581 ttaaggcccc ttcctcggga ggggcttgcc cgtttcagcg tcccgctgaa atcgtcggcc
    14641 tacctccttt tgccctgaat gcagtctatc gctaacgcgt agataaaata gtttcctgtg
    14701 ttattactgg atgggtgctc gcaaatgtgc ccgccattca gacgattccc gatagtgttt
    14761 cataattcct ccatttttct ccctgattgg ctggctacac tagtatcatt ccgcgaatcg
    14821 tttcaggaag agaaactctt aacgatgaaa agcagttata aatcccgttg ggtaatggta
    14881 atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg caatgactcc
    14941 cggagtgcag cgccaggggc gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt
    15001 ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga acaggcagtt
    15061 ccgcgttacc tcaccgggct tggcaccatt accgccgcta atactgttac ggtgcgcagc
    15121 cgcgtggacg gtcaactgat ggcgttgcat ttccaggaag gccagcaggt caaagcaggc
    15181 gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca agcccagggc
    15241 caactggcaa aagataaagc cacgcttgcc aacgcccgcc gtgacctggc gcgttatcaa
    15301 caactggtaa aaaccaatct cgtatctcgt caggagctgg atgcccaaca ggcgctggtc
    15361 agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc gcagctgcaa
    15421 ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat
    15481 gttggtaacc aaatctccag tggtgatacc accgggattg tggtgatcac ccagacgcat
    15541 cctatcgatt tgctctttac cctgccggaa agcgatatcg ctaccgtagt gcaggcgcaa
    15601 aaagccggaa aaccgctggt tgttgaagcc tgggatcgca ccaattcaaa gaaattaagt
    15661 gaaggcacgc tgttaagtct cgataaccaa atcgatgcca ctaccggtac gattaaagtg
    15721 aaagcacgct tcaataatca ggatgatgcg ctgtttccca atcagtttgt taacgcgcgc
    15781 atgttagtcg acacagagca aaacgctgtg gtgatcccaa cagccgccct gcaaatgggc
    15841 aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg
    15901 acgccgggca ttcaggacag tcagaaagtg gtgatccgcg caggtatttc tgcgggcgac
    15961 cgcgtagtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga agtagtggaa
    16021 gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga
    16081 gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg cgcctgttta
    16141 ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc gggattatcg
    16201 gttatcgagc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc attcaggtag
    16261 tcacgctcta cccaggtgcg agcccggatg tcatgacctc tgccgttacc gcgccgcttg
    16321 aacgccagtt tgggcagatg tctggcctga aacagatgtc gtcgcaaagt tccggcggtg
    16381 cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc gagcaggaag
    16441 tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct aacccgccgg
    16501 tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgctgtc acctcaaccg
    16561 ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc
    16621 agatttccgg cgtcggcctg gtgacacttt ccggcggtca gcgtccggct gttcgcgtca
    16681 aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca
    16741 ttaccggcgc taacgtcaac tcggcaaaag gtagcctcga cggcccttct cgtgcggtta
    16801 cgctttccgc taacgaccag atgcaatccg ccgaagagta tcgccagcta atcatcgcct
    16861 accagaacgg cgcgccgatt cgtctgggcg atgtcgcaac cgtagagcaa ggtgcagaaa
    16921 acagctggct tggcgcatgg gctaacaaag aacaggccat tgtgatgaat gttcagcgcc
    16981 agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg ccacagctca
    17041 ccgagagtct gccgaaatcg gtgaaggtga cggtactttc tgaccgtacc accaatattc
    17101 gcgcatccgt ggatgatact cagtttgaat tgatgatggc tatcgcgctg gtagtcatga
    17161 ttatctacct gtttttgcgt aatattccgg cgaccatcat tcccggcgtc gcagtgccgc
    17221 tgtcgttaat cggcaccttc gcggtcatgg tgtttctcga tttttcaatc aataacctga
    17281 cgctgatggc gttaactatc gccaccggat ttgtggtcga tgacgccatc gtggtgatcg
    17341 aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgct ggcggcggcg ctcaaaggcg
    17401 caggtgaaat cggttttacc attatctcgc tgaccttctc gctgattgca gtgctgatcc
    17461 cgctactgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct attactctgg
    17521 cagtcgcgat tttgatctca gccgtggtgt cgctgaccct gacaccgatg atgtgtgcgc
    17581 ggatgctcag ccaggagtcg ctgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa
    17641 tgttcgacag gataatcgcc gcctatggtc agggacttgc gaaagtgctg aatcatccgt
    17701 ggctgacctt aagcgtggcg ctcagcacgc tgctgcttag cgtgctgttg tgggtgttca
    17761 ttccgaaagg tttcttcccg gtacaggaca acggcattat tcagggcact ttgcaggcac
    17821 cgcaatccag ctcctttacc aatatggccc agcggcaacg ccaggtcgcg gatgtgattt
    17881 tgcaagatcc ggcggtgcaa agtctgacct catttgttgg cgttgatggc actaacccgt
    17941 cgctgaacag tgcgcgtttg cagatcaacc tcaaaccgtt ggatgaacgt gatgatcggg
    18001 tgcaaaaagt catcgcccgc ctgcaaacgg cggtggataa agtgccgggc gtcgatctct
    18061 tcctgcaacc aacgcaggac ctgaccatcg atactcaggt cagccgcacc cagtaccagt
    18121 ttaccttgca ggccacgtca ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa
    18181 aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac aaagggctgg
    18241 tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc atggcggacg
    18301 tcgataacgc cctttacaac gcgtttggtc agcgactgat ttccactatt tatactcagg
    18361 ccaaccagta tcgcgtggtg ctggagcaca acaccgaaaa tactcctggg cttgcggcgc
    18421 tggatactat tcgcctgacc agcagcgacg gcggcgtggt accgctaagc tcaattgcca
    18481 aaattgagca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc ccggtaacga
    18541 ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag gcgattatgg
    18601 acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcagttccag ggcagcaccc
    18661 tcgccttcca gtcggcgttg ggcagcactg tctggctgat tgtcgcggcg gtggtggcga
    18721 tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc attctctcga
    18781 cgctacccac cgcaggggtc ggcgcactgc tggcgttact gattgccggt agcgagctgg
    18841 atgtgattgc gattatcggc attattttgc tgatcggtat cgtgaagaag aacgccatca
    18901 tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg cgcgatgcaa
    18961 tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg gcggctctgc
    19021 ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag
    19081 gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc accacgccgg
    19141 tgatttatct gctattcgac cgcctggcat tgtggaccaa aagccgcttt gctcgtcatg
    19201 aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg gcgacgattt
    19261 tactgtcggt cgccattacc ctgtgcggca tactgggttt ccgtatgctg ccagtcgccc
    19321 cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac
    19381 cagaaacaat ggcgtcttcc gttgccacgc cgctggaacg ttcgcttgga cgcattgccg
    19441 gggtcagtga aatgacctcc agcagttcgc tcggcagcac gcgcattatt ttgcagtttg
    19501 attttgaccg cgatatcaac ggcgcagcgc gcgatgtgca agcggcgatc aacgccgcgc
    19561 aaagtttgct gcccagcggg atgccaagcc gtccgaccta tcgcaaagcg aacccgtcgg
    19621 atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt gaactgtacg
    19681 atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtc ggtgatgtcg
    19741 atgttggcgg cagctcactg cccgccgtac gcatcgggct gaatccgcag gcgctgttta
    19801 atcagggcgt gtcgctggac gatgtgcgca gcgccatcag caatgccaac gtgcgtaagc
    19861 cgcagggggc gctggaagat gatacccatc gctggcagat ccagactaac gatgaactga
    19921 aaaccgccgc cgaatatcag ccgttgatta ttcattacaa caacggcggc gcgcttcgtc
    19981 tgggcgatgt ggcgacggtg accgactcgg tgcaggatgt gcgcaacgcc gggatgacca
    20041 acgccaaacc ggctattttg ctgatgatcc gcaagctgcc ggaagccaat attatccaga
    20101 cggttgacag catccgggca aaattaccgg agttgcagga gaccattccg gcggcgattg
    20161 atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggag gaagtcgagc
    20221 aaacgctgat tatctcggtg gcgctggtga ttctggtggt ctttttattc ctgcgctcgg
    20281 gtcgcgccac tattattccc gccgttgcgg tgccggtttc gctgattggt acgtttgcgg
    20341 cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc accatcgcta
    20401 ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcccgt cacctggaag
    20461 cggggatgaa accgctacag gccgcgctgc aaggcactcg cgaagtcggt tttacggtgc
    20521 tgtcgatgag tctgtcactg gtggcggtgt ttctgccgct gctgttgatg ggcggattgc
    20581 cgggtcgcct gttacgcgag tttgccgtga cgctttctgt cgccattggt atttcgctgc
    20641 tggtttctct gacgctaacg ccaatgatgt gcggctggat gctaaaagcc agtaagccgc
    20701 gcgagcaaaa gcgactacgt ggttttggtc gcatgttggt agccctgcaa caaggctatg
    20761 gtaagtcgct gaaatgggtg cttaatcata cccgtctggt gggcgtggtg ctgcttggca
    20821 ccattgcttt gaatatctgg ctgtatatct cgatcccgaa aaccttcttc ccggagcagg
    20881 acaccggcgt gttgatgggc gggatacagg cggatcagag tatttcgttt caggcgatgc
    20941 gcggtaagtt gcaggatttt atgaaaatta tccgtgacga tccggcagtg gataatgtca
    21001 ccggatttac cggcggttcg cgggtgaaca gcgggatgat gtttatcacg ctgaagccac
    21061 gcggcgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgtg aagctggcga
    21121 aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt ggcggacgtc
    21181 agtcgaacgc cagctatcag tacacgttgc tgtccgacga cctggcggca ctgcgcgaat
    21241 gggagccgaa aattcgcaaa aaactggcga cattgccgga actggcggac gtgaactccg
    21301 accagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc atggcacggc
    21361 tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt cagcgacaaa
    21421 tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg gatccgcgct
    21481 atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa ggaaaagcga
    21541 tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc tccgctgtcg gtgaatcatc
    21601 agggattatc ggcagcctcg accatctcgt tcaacctgcc gaccgggaaa tcgctctcgg
    21661 acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg
    21721 gtagttttgc cggcacggcg caggtgttcc aggagacgat gaactcgcag gtgatcctga
    21781 ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag agttacgtac
    21841 atccgctgac gattctctcc accctgccct cggcgggcgt tggagcgctg ctggcgctgg
    21901 agctgttcaa tgccccgttc agcctaatcg ccctgatagg gatcatgcta ttaatcggca
    21961 tcgtgaagaa aaacgccatt atgatggtcg attttgcgct tgaagcccaa cggcacggta
    22021 acctcacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc ccgattatga
    22081 tgactaccct ggcggcgctg tttggcgcgc tgccgctggt attgtcgggc ggtgacggtt
    22141 cggagctgcg gcaacccctg gggatcacca ttgtcggcgg tctggtcatg agccagcttc
    22201 tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcga ctgcgttttt
    22261 cgcgtaaacc taaacaagcg gtaaccgagt aaatgacaga tcttcccgac agcacccgct
    22321 ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtta
    22381 acaccgcgct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg
    22441 tcattgtctc ttatgtactg accgtggcgg tgatgctgcc cgccagcggc tggctggcgg
    22501 acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact ctcggttcac
    22561 tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg ttacagggcg
    22621 ttggcggcgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc gtaccgcgcg
    22681 agcaatatat ggcggcgatg acctttgtca cgctgcccgg tcagatcggg ccgctgcttg
    22741 gcccggcgct cggcggtctg ctggtggagt acgcatcgtg gcactggatc tttttgatca
    22801 acattccggt ggggattatt ggcgcgatca ccacactgat gttaatgccg aactacacca
    22861 tgcagacgcg gtgctttgat ctctccggct ttttattgct ggcggttggc atggcggtat
    22921 taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg attgctggcc
    22981 tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga aataacaact
    23041 gcgccctgtt cagtctgaaa ctgttccgta ctcgtacctt ttcgctgggt ctggcgggga
    23101 gttttgccgg acgtatcggt agcggcatgt tgccctttat gacgccggtt ttcctacaaa
    23161 ttggccttgg cttctcaccg tttcatgccg gactgatgat gatcccgatg gtgctcggca
    23221 gcatggggat gaagcgaatt gtggtacagg tagtgaatcg ctttggttat cgtcgggtac
    23281 tggtggcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact actgctctgc
    23341 tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc aactcgaccc
    23401 gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg
    23461 gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc actatcgccg
    23521 ggctgttgct ggggcttttt ggttcacagc atgtcagcgt cgacagcggc accacacaaa
    23581 ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg gcgtttgtct
    23641 ttgccagagt gccgagcgat acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg
    23701 cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga ttttcgccac
    23761 ctgcattgtc ttattgatca cgatgcactg ggcggtacgt atcagttttg agcgcggctt
    23821 tatcgattac atcaagcatg gtaatgaaca acggctgcaa atgctcggcg atgcgcttgg
    23881 tgagcagtac gcccagcacg ggaactggcg cttcctgcgt aataacgatc gctttgtatt
    23941 tcagatccta cgttcactgg agcatgataa caacgaagat aagcccggcc ccggtatgcc
    24001 accacacggc tggcgcacgc aattttgggt ggttgatcaa aataataaag tgctggttgg
    24061 cccgcgagca ccggttccac ccgacggcac acggcggccc attatggtca atggtgcgga
    24121 agttggcgcg gtgatcgcct cccctgttga acgactgacc cgtaatactg atatcaattt
    24181 tgacagacaa cagcggcaaa ccagttggct gattgttgct ttatctacct tattagcggc
    24241 gctggcgaca ttcccactgg cgcgcggttt gctggctccg gtaaaacgac tggtggatgg
    24301 cacgcacaaa ctggcagcgg gtgatttcac tactcgcgta gcccccacca gcgaagatga
    24361 gttgggcaga ctggcagagg atttcaatca actcgccagc acactggaga aaaaccagca
    24421 gatgcgccgc gattttatgg ccgatatttc tcatgaactg cgcacgcctt tagcagtact
    24481 gcgcggcgaa ctggaagcca ttcaggatgg tgtgcgtaaa ttcacgccgg agacgctggc
    24541 ttctttacag gcggaggtcg gtacactgac caaactggtt gacgatcttc atcagttgtc
    24601 gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg gtagatttga tcccactgct
    24661 ggaagtggcg ggcggcgcat ttcgcgaacg atttgccagc cgtggcctga aactgcaatt
    24721 ttccctgcca gacagtatta ccgtatttgg cgatcgcgat cgtttaatgc agttgttcaa
    24781 taacttactg gaaaacagcc tgcgctacac tgacagcggc ggcagcctga aaatctctgc
    24841 cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agcgcgccgg gcgtcagtga
    24901 cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc gaaggttccc gcaaccgtgc
    24961 cagcggcggt tccgggctgg gactcgcgat ttgcctgaac attgttgaag cacataatgg
    25021 ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag agttaccgct
    25081 ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa cacaccgcgt
    25141 attttgatcg tggaagatga accgaagctg ggacagttgc tcattgatta tctgcgtgcc
    25201 gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta tgtgcgccag
    25261 acaccaccgg atctgatcct gttagatctg atgctccctg gcaccgatgg cctgacgctg
    25321 tgccgggaaa ttcgtcgttt ttctgacatt ccgatcgtga tggtgacggc aaaaatcgaa
    25381 gagatcgacc gcctgctggg gctggagatt ggcgccgatg actacatctg caagccgtac
    25441 agcccacggg aagtggtagc gcgtgtcaaa accattttgc gccgttgtaa accgcaacgc
    25501 gaacttcagc aacaggatgc cgaaagcccg ttgatgatcg acgaaggtcg ttttcaggct
    25561 tcatggcgcg gtaaaatgat tgacctgacg cctgcggaat ttcgtctgtt gaaaacgctc
    25621 tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct ttatgacgac
    25681 taccgcgtag taactgaccg caccatcgac agccacatta aaaacctgcg tcgcaagctg
    25741 gaagctctgg acgaagagca gtcttttatc cgcgccgtct acggtgtcgg ttatcgctgg
    25801 gaagccgacg cttgccgcat cgtttagttt tagcgacatg actttgttag ccggagatgg
    25861 cgttcagatc agatccggca acattatccc acgtatggtc agcaaactga cattatctct
    25921 ccgccctggc aggcaccaga aaatcgctca tactttaatc ggtaaacagc acctttagat
    25981 gctgttttcg atacgcaatt tcaatcaagg agtcattatg gctggttggt ttgaactcag
    26041 taagagcagt gataatcaat ttcggtttgt gctaaaagcg ggcaatggtg agactatcct
    26101 caccagcgag ctgtatacct caaaagcctc tgcggaaaag ggcatcgcgt cggtgcgtag
    26161 caacagcccg caagaagaac gctatgagaa aaaaacggca agcaacggca aattctattt
    26221 caatctgaaa gccgccaatc atcaaattat cggctccagc cagatgtacg ccaccgcgca
    26281 atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga cggtgaaaga
    26341 caatacgtaa ttacgatgcc gggcgcgtgt tgttggcgtc cggcgcttta ccagctcccc
    26401 cctacagcgc tacaatgccc gcccttaaag tgggggcact cccctggccg cttcatcagg
    26461 tgaagcggat ctgacctgtc atcagaacga gagaattatg tttaaaccgg aactcctttc
    26521 cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag atgctgttta
    26581 tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac gaattcaacc acgaaaatct
    26641 tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg tggtcaacat
    26701 tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg tggtggaaat
    26761 ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc gtgagcactt
    26821 ccctgaaatg ccgatccatc tctcggtaca ggctaacgcc gtgaactggg cgacggtgaa
    26881 attctggcag caaatgggcc tgacccgcgt gatcctctct cgcgagctgt cgctggaaga
    26941 gattgaagag atccgcaatc aggtgccgga tatggagatc gagatcttcg ttcacggtgc
    27001 gctgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca agcgcgaccc
    27061 gaaccagggt acctgcacca acgcctgccg ctgggagtac aacgtccagg aagggaaaga
    27121 agatgacgtt ggcaacatcg tacacaagta cgagccaatt ccggtgcaaa atgttgagcc
    27181 gacgctgggt atcggcgcgc caaccgacaa agtgtttatg atcgaagaag cccagcgtcc
    27241 gggcgagtat atgaccacgt ttgaagatga gcacggcact tacatcatga actcgaaaga
    27301 tctgcgcgcc atcgcccatg ttgaacgcct gaccaaaatg ggcgtgcatt cgctgaaaat
    27361 cgaaggccgt accaaatctt tctactattg cgcacgcacc gcgcaggttt accgtaaagc
    27421 tatcgatgac gccgctgcgg gcaaaccgtt cgataccagc ctgctggaaa cgctggaagg
    27481 tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catacccacg acgattatca
    27541 gaactacgaa tacggttatt cggtttctga ccgccagcag tttgttggtg agtttaccgg
    27601 tgagcgcaag ggggagctcg cggcggtagc ggtgaaaaat aaattctccg ttggcgacag
    27661 ccttgagcta atgacgccgc aaggcaacat taactttacc cttgagcata tggaaaacgc
    27721 caaaggtgaa gcaatgccgg tcgcaccagg cgatggttat actgtgtggc tcccggtgcc
    27781 gcaggatctc gaactgaatt acgcgctgct gatgcgtaat ttctccggtg aaaccacgcg
    27841 taacccccac ggtaagtgat taatttcgat tatttttccc ggatgaaaaa ttcttagaaa
    27901 ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa aacataaccc
    27961 ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc cttacacggg
    28021 cttatttttt acgcgtaata caatgaaata aaaggattta tttctggtca cgtccacaca
    28081 ttgaccacat cgacaaaaaa gcccctcgac tgaggggctt cctgtttgta attacatcca
    28141 cataatttgc tgccctgacg gcaacgggtg cggtcttacg gcgtggactt ctcccggctt
    28201 cacgatgtat cgctgtaccg actcataagt gatgaacgtg gcgctgcaat tcacgttctg
    28261 gcactggtga taacgctctt ttgtcgtgtc agtgatatag cgacttgtac gcgcatgtgc
    28321 ggcatgctgg cataaaggac aatgaaacat cgcgagcacc tcttccggtt ttgttgatgg
    28381 tgccatttta gttaatttat ccttataaaa caaacagata aaacaaaaac atcactcatc
    28441 atcttctgtt tcgtactcca catcagaaag cctgacctca agctccaagg acgtcgtgaa
    28501 gccgctatta ttcagaaaat gtgtcacctt agtgattgtc cagtcctgct cgtctatgac
    28561 gcgcttaaag cctgacactt tgaccggcgt ttccgtgtaa atatctgccc gaccagtagc
    28621 cagactgatg gagaactccg caacgccccg ttgcagctta tcccacttcg cctgagcggc
    28681 gcgcatggcc tgtgctttcg tggcatatac cgtggtcagg gcaaaaacgt tgtcagcctc
    28741 accagccatg tattcacctt cgcgcgcttc cggtactttt ggcgctttct tctgcgtgac
    28801 tggtttcgct ttcgggtgct ccagtgcgcg caggtgtttc tctttctttt tgcgtttcag
    28861 ttttaccttc tgcttttgtg gcttcgggtc tttggtgtgt aaccactttg ccgttacgcc
    28921 ggtataggct ccacggtcag caatcgcaaa atgatggcgg tcgccgtcgc tgcgggttat
    28981 ggtgacctgc gggatttttt taccgctggc cgtcaccccc tgccccgctt tgagaaacaa
    29041 cagttttccc atttttaccg acacctcacc gccgttgcgt tctgcaagac gggtcaggaa
    29101 tttcgcatca gactcctgcg actggtcgat gtgcgggatt ttaattccgg ccagtgacgg
    29161 agcgacactg gcttccagcc tgttacggga ggctatcgcc tcaacaatcg caccgagcgt
    29221 ggtgtcatgc caggagcctt cacggcggga attgagcgtc ccgcgaaaat ctgcactccg
    29281 ggcgcggatg gtgaccacat ccggtgcgcc ccggtgttca acctcatcaa cggtaaattt
    29341 ccctttgcat accagggcaa aacctttcca gccgatatac accgtcagga cagcgccacg
    29401 aaccggcagc ccgacctgcc cgtcggcatc gttcagttca atatcaagct ggtcagcctc
    29461 aaagccccgg ttatccgtca gggtcatgct catcagtcgg tcgctgatat tgccggaaat
    29521 atccctgctg tcgagcatca gcatgtaatc cggcgtcagc gtactgcctg catcaaatgt
    29581 cagtgcatcc agcattatcc cgcccccgtc atacccgtga atctggtcgc catactgcca
    29641 gccttaccga tgagcgattc cgcctgttta ccgatatcgc cataaagcgc ggccagtgat
    29701 tcatcaacgc gggtgagcga cagcgtaaaa tcaatttttc ggggtgtgcc gtctgcaaag
    29761 aaaatacttc ctgtttcact caccctgctg atgacataca tgccgtaaat catgccggtg
    29821 ccatccagca acggccacgc ccggccttcc tctgccatca gcctgagcgt ggtcatcgtc
    29881 agcttgccgc cggtcagttc tggataaagc acaccggcaa gcgtgatgtt ttcctcaccc
    29941 acaccgagaa actggtaggc gtcccgttta ccgatacggg aatttgacgg ccagcgataa
    30001 tctgattcac gctgcatggt ctggtgtggc agcgtctggc gcataaaaac aaacatacct
    30061 aacgcgagca tcatttttcg tcacctcctt aaccgtcatg catcatgctg gcacgggcgc
    30121 gcgcacgttt atcccgctcg tatttttcga gcgcatcctg taactggcgg tcaagctgtg
    30181 tccccggcgc agtaccaccc gtcaggctga tgtgatattc gtttttactc tggtccacat
    30241 aagagcggcc agccggtgcc gtgaccggct gataagcctg atagcctgca taagagctgg
    30301 tcgccggaat ataaccaccg gtgccatacg tggcggcatg agttctggcg gcggtctggt
    30361 caagtgtgtc tgactctttg ttgataacac cgagtttttc cagtacccag tcaataccgc
    30421 tgcgcagttt gttgaacgca ttaagcggca gcatcagcgc gtcagccagt gcctgcccga
    30481 acatgacgcc cgtgtcacgg caacggttca gggtgtcctg tgtggcttta accggagcaa
    30541 tcaggtttgt aaaccactgc cacgcggcct gtaacttttc gcccagccag tcaaacaccg
    30601 gcttaagtgg cgtgaacagc tcccccaccg gcgcaaatgc cgctttcagc ccttcaacca
    30661 caccgccaaa gaatgcgctg acaggctccc agtatttacg gataagcaac gccccggcga
    30721 caatggcagc caccacggcc acaaccggcc agctaatcgc accgatggcc gtcataacgg
    30781 cactaccaac cgtcgtgaag attgccccca ttgcgcctgc tgccgcgata atggcattga
    30841 tgccggtgat aaccggccag gctacaaggc caatggcacc gatgatgcca gtcagcacca
    30901 gtgcaccgcc ggcaatgatg ccgatggttg acgccagtga tttgtttttc tgtatccagc
    30961 cgtcgagttt taacacatac tttgtggccg tctgcgtgag cttacgcagt gcgccttcct
    31021 gctggtcaaa caggtcagtc cccaccgcct cataagcgga ctgaaactcc ttaaagtcac
    31081 cgccgagatt gtcctgcata atatttacca gctctgaggt cttcccgtcc gaggctttaa
    31141 acgcagcggt cagtttgtcc agctttccgg ttgaggcgac agtcatcagc acggcggcgg
    31201 ctgagctggc ctcctccccg aaaatggttt tcatgtattc agcctgctgg gcagtaccga
    31261 gccggttttt ctcaaaactg gcctgcattt ctttcagaat ggtaaatact ggtcgggtgt
    31321 ttcccttgct gtctgaggtt ttcactccaa gctctttgag tgcatcccat gcttttcccg
    31381 tcggtgcctg caggcggctt aacacggcac ggcttcccgt ccccgccatt gagcctgtga
    31441 tttttgcatc atgcagcgcc ccgaccattg cggcggtttc ttcaatgctg acaccggcat
    31501 tttttgccac aggtgcggca taggtcagcg catcgctcat gccgtcaaaa tcggcggcgg
    31561 ttttgttcat cgtcatggag agaacatccc cgatatgagc gaccttatcg tttgaaagct
    31621 gaaaggcgga tttcatcccc atcagcaggg cggcgttttc ttccatcgtg cggcggttcg
    31681 ccagcgccat attcagcgtg accggcgttg ccgcctgaat ggcatcaaca tccccacccg
    31741 ctttcgcgat gattatctgt gcaccggccg catcatccgc cgaggcggcg gtattgtcgc
    31801 cgagctggcg cgcctgtttg cgtagtgcgg tcatttcggc ggagtctttt gccacaccca
    31861 gcacggcctg caattctgag tttttctgcg caaactcata accggacatc agcaacttaa
    31921 ctccggccat cgtttccgcc gccgcaatcc ccacaccggc agcgcccact gaggccatat
    31981 ttccggccag ttcctttccg gcctgataac gctgttttac tgcattaagt tttgcctgtt
    32041 gcgcactgac acgcgccagc gcgtcacgct gccggttaag ctgtgcggtg gtttcactga
    32101 tacggttttt cagtccctgc tcatcatgtg caagattgcg ggtattaatt cccacagcgg
    32161 ccagttcccg ctgctggcgc ttaacggaat ccgtcaggcg gttatatttc gcctgtaagt
    32221 cctccgccgc acgctttgcg gattccagca ctttcgcctg agcacgggtc ggacgttcag
    32281 tgtttttaaa ctgtgtggca agtgcttcgg cttcctgccg tgccttttca agtgcatgac
    32341 cagtcacggc aagctgtgcg ctggtcttgc gaaatccctc aatacgggat gcgtgaccgt
    32401 tcagctcgcg cagtgatttt tgtgtttccc ggatatcccc cgacagcgac ttactcgctg
    32461 tacggatgga tttaaacggg cgggatgcct ggtcaacagc cctgagcaat acctgtaatt
    32521 ttacattgtt actcattcgt gcttccgctt cgccggagcg ccttttcgcg ccatgtgatg
    32581 agttcggtca ggctcatggg atacagttct gatggcggcc agtgaaatat cactgccaca
    32641 tctgccatca ggtcatcgac cgacagattt ttcggaaacg tcactgcacc gagttcggag
    32701 acaaaaaacc gaccacctta ccggccagcg ccacaaggtc aggcagttcc agcgcggcga
    32761 cctcctgctc ggtcagcatc ggtgccgtca tgcgcggcag caccttaatc agtgcatcga
    32821 cttcggagtt tgcgaccgca gccagactga caccgcgcag cgtcccggca ttaggtttca
    32881 tcagcgtgac ctgttcgata acctgctcac cacgcttgac cggattgtcc agggtaatca
    32941 cattttcttt gttcatggtt ttctcacttc tgaatcgggg ttaaccggtc agccaggctg
    33001 accggatgaa aatcacaggc cgatattgcg gcggtgttgc tccagccggt cgacgccgtt
    33061 caccttctca atcatgttga tggtgtcgat ttcgaccagc tccttaccgt ccatcgtcaa
    33121 cttgaaataa gtacaaatca ccgggagttt cgattcggta tcctctccct gtttaccctc
    33181 tccggtatcg acttctttct gacgcccacg catgaccact tcgacggcca ccgtttcgcc
    33241 ggtatcgtcg cgctggtaag aaccagcaaa acgaatcggt acggcatcag cactggttgc
    33301 agcgtaaagc tcccagataa ccgaatccgg gaatccaccg agcgaccact ccattgacag
    33361 agcatcatca tcaaggccga ggtctaccgg tgcgctgccg ttcatccccg caccgcgata
    33421 gttttcgagc ttacgggtca gttttggcag cgtgacggac tttgcaacgc cctgatagct
    33481 gtagccgttc agaaagacgt tcattaactt gagtttgcgc ggcattgcca tcggtcaggc
    33541 tccttaattg ctgttaaccg aagtgaccag acttgccagg tatctggaag taatacgctg
    33601 gcgcagggtc aggttttcga gaggaggcac cggggtatag tcgtagtcga tatacagttt
    33661 tccggccttg agggtttccg catcgttgga ttcttcgctg aaccagcatg tgccatccac
    33721 gatataaccg ttgtttttca gttcacggaa tttggcattg atgccgtcaa tgatgtcgcg
    33781 aatcagcgtt gcggtgatgg gcttatcaat agcccacatg tgcgcctcag ccatcgtgtc
    33841 ggccagtacc tgcgcggtgc gtgtgtagct ttcaaagagg aacagcgggt catcagagca
    33901 ggtacggtta ccccagaaac ggaaaccgtc gcggcgaacc agcgttgtga cgcctgcctc
    33961 gttcagcagg tcagcatcag tgcctggctt ctgcaaatcc cagaagacgg atgcactgat
    34021 gccggtaaca ccgttcaccc cgacgttgga cagcgtttta tgccagccct gctcctggtc
    34081 gattttagcg cgcagaccca gcgcacgagc ggtggcatac gcggtggcgg tggtactggt
    34141 aaccgtatcc catgcgagga aatccggcca gataaccatc agctcacgct ggctgaaatt
    34201 ctggcggtag gctttcacct cggaaatggt tttacaaccc catgcgctga tatacccgaa
    34261 ggcattcagt tcctggcata ccgatgccag tgcgacggcg acctctttgg tgtccagtcc
    34321 cggtacgccg agaatgcgcg gtttaacgcc ggtaaccgac tcagccccca tcagggcttt
    34381 cagtccggtg tactgaccgt tttcgtcggt ggtgccgata atattggaaa cagtctgcgc
    34441 gagtttcgtt tcctcgtcgt cgccggtgcc gtcttccacg cgcacaacaa cggtgaccgg
    34501 ttttgactgg tcagcgatgg cctgtaacga cgccgccagc gtgccttttt taccggcctt
    34561 tgcaattgcg ctctgcacat tggtaatcag cacaggttta ttgaggggga atgtttccgc
    34621 atccgcatcg ctggccgtgc agaccatgcc gacaatggca gtggatacgg tggaaatgac
    34681 gcgggtgccg tcgttaatct ccagcacctg cacgccgtga tgatagtcac tcatccgtct
    34741 aactccgtgg ttaatgggtg agtggtattt tctgttgtgc agagcatgag acgctatttg
    34801 acctggctgg tcagtggatg aaacaacaga taaagaaaag gcggacaatc cgcccgcctg
    34861 tcatgatttg tactcactta tttcccgact gacaatttac atagccaaaa cgctatcaaa
    34921 tctgacagtc tgctttgagc gagaagcgga agttcaatgt gataaagctc aggaacattg
    34981 tcgttgatga tgataaagag gatcagacta acttagcact tctcatcacg ttgataaggc
    35041 atagaaactg caaacactat tgtgaatata atttcactat agtaaattaa agtaagctct
    35101 tttcgttcgt tattacataa aataaagttc tctctaaggt tgaaaaatgt tagatatcag
    35161 aatgtcacga ccatctgaag catcagagat catacaaatc tggaagagtt cagtggatgc
    35221 tacccacgac tttcttacag gtcatgaccg acaagaaatc gaaaaagaag ttatcggctt
    35281 tttctcagaa actcctgtat gggttgcaac aaatcaggat gaccaaccgc taggatttat
    35341 gtttttgcat gaggggcatc tggaggcact ttttgtagca ggctctgctc gcggacttgg
    35401 cgtcggaaaa cgtttgatat ctcatgcact ggcgctgcat ccagagctaa gcgttgatgt
    35461 aaatgaacaa aaccagcagg ctgtagggtt ttatcagcat atgggttttc aggtatcagg
    35521 gcgttccgat ctggataatc agggaaggcc ataccccttg ttgcatctga gaatggccaa
    35581 taacatgtaa ccaatgtggc agagaagata tcattgattt ttttggctaa aagccacgat
    35641 gtaagaacag ctctgctcac tatctggatt gctcatttgg tactcgcttg caaaggtaag
    35701 ggcaagacag tcaccggcac gtaaatgatg ggtctgattt ttgactcgaa aatcaaggct
    35761 gccactcatc atccaaagcg tttgcccgga aagatgctca tttgcggagg cgggtatcgt
    35821 cagctcaccc attgcaggga tttcaacttt gatcagttcc gggcatgccc ccgcaggaga
    35881 caaagaccaa cgggtgatgc ctgattgctc atccgtccag tgctgttgct gatgagcaaa
    35941 acgaaccagg gaattctgat gttgctcaag ctctgcaaag agtttagata gcgttacatt
    36001 catggcgttg gccaggcggc taaggatagt tgcactaggg ctggagatgc ctcgttcaat
    36061 cttactaatc atggcctgac tgacgcccga tcgttgagca agctctgtta cagtcaaatt
    36121 gcgagccttc ctgtgtttaa gcaataaacg tgcaatatcg ctatctacta tctgattttt
    36181 aaatttttta tccatttccg ccctctcttt caccaacctg attatgccat tacataaatt
    36241 ggcctgaaat aaatgatttt gtaagtctgt aaattcacta ctcctaagcg cggcacttcg
    36301 tttatacgca cctataacct taaaatatga gttggtaagc ttaacgtccg cttttggcac
    36361 atagcggact gtcagattag gctttactct gtgctataga tatgtaagct cacaccagag
    36421 atcatacaac ttattgcggc atttccggcc attcgggatt tgcaggatcc acacgactga
    36481 ccagaacgct gtagcgttcc catgcttcca gtcggctgcg ttcctcatcc gtcgccatat
    36541 tcagcctgac agcgcgttcc agcggcaaaa tcacggattc agcttcggaa agtaaagctg
    36601 ccttttgtga ttctgcctgt tgctcctgtt catctgccgt ataaatccgc ttaatcacgg
    36661 caccatcctt aaacatccat ttacctgagt catcagcacg tcggttggag gtaatatcag
    36721 gaacctcgac aacgctgaaa ccttcagggt taagcgttga agcatctctg gtgatgccga
    36781 caattatatt attctcgtcg taaacaatct ttatcgtgtc ttcctgaaaa ttacttactt
    36841 cctcatacca gttttttccc tcttcggacc ataaccagat aacatcaaaa ttttttgtca
    36901 gttgatattg ggcaacagtt tttggattac ccgcagtaat attttttaaa tgctgcataa
    36961 attacacctg tgcgacgtta taccatgtgc cattgatgta tttttgtatt ggcctgaata
    37021 ctgcggggtc atcaccatcg acttcaccga caataccaag cccggtaatt acgtgccctg
    37081 ctttctcata catcacccct ttctgcatgg tctggacaac acgtgtgcca agtctgatat
    37141 ctctcacata gcgggaatca aagttaccgt aatccgaggg attaacacgc cccgtaatat
    37201 ttatggtctt attactttga atgcttccgg aaacaaagcg cataacatgg acgttattag
    37261 cataaacatc cagattacca tcgccatttt gtttaaatcc ggtgtcgtta tcaccaagaa
    37321 caatagagtt cccacccaac gcgttattcg tgccaagtgc cagcccgcca tcaatcctgg
    37381 caccattacc aacagacaca actcctgttc ttaagttgat gccgaatggc cttaatgacc
    37441 caatatctcc attttcaccc tcattttctc gtgtaggaat gatatacagg ttttcttcag
    37501 aacggcgaaa aatagcacca aaagatgaat taaatatcct cagcgcattg actgtagata
    37561 tttttacttc actactgaaa agggctttaa caagaacata cagagcatcc catttaagat
    37621 tcatcaggtc ttttgttgtg gtgctctggc gacttctcca tttgaaatat tcattgccgt
    37681 tgtcacctgt ttcaaaccac atatatgaat cagtatcgct gtcggcatca tttttaaatc
    37741 caatctttgc ccagtcagta tttcgaatcc aggcaaggat tgagtcgttt tcaaaagtaa
    37801 gcccgccgga caaggtatcg ccattttttt gcacggcgtt cccggctcgg tttaccgttt
    37861 cctgtaaacc gagatattcg ataacggcgg caacggtcga tttagccaga atatcccgcc
    37921 cgacttttgt cagggtcgcc aggctggcaa catcattccc cgtaaaatac ggaaacctgt
    37981 ctgccgcagt agcaagcccc gccagcgccg tcaaagtggc atctttcggt tgcttacccg
    38041 caagcgcatt agtcatggtg gtagcaaaat tcgggtcatt gcccagcgcc gccgccagct
    38101 cgttcagcgt attcagtgcg tcaggcgacg agtctacaag ggcggcaatc gcggccataa
    38161 cgaaagccgt gtttgcgatc tgagtattat tcgttccctg tcgcgcagtt ggcgtcgttg
    38221 gcgttccggt cagtgcaggg ctgtttaatg gcgctttctt gttcgtttca tccattaccg
    38281 tcttaacggc ttttggtgtt gcggcgagcg tttcagacat gctgttggtc gcactactta
    38341 gctggacaag accttttcgc gctgtggtag cgtcctgtgc ggtatatttc ccgttagcaa
    38401 ggtcatacgc ggcctttacc gctttcggcg ttgcggccag tgtttcagac gtgctgttgg
    38461 tcgcactgct taactgagta aaacctttgg cggtcagcga ggcgtccggg tgacgtcgtg
    38521 actgttcgtg ctctgcaatt ttgtcatcaa cgtaatcctg tgtcgccatc accgttgtgg
    38581 tgtcaatggt cagctccacc gaggccacac tgctgacgat gatgaccatg cgacaggtct
    38641 gcgaacgccc tgagccttcg gcaagagctg gcttataact ttcggccatg ttcgccacgg
    38701 caattaacgt tcccgcatca tcgtacaggc caagctcacg catccagaaa ccgcccacct
    38761 ccggcggaat aaccagctct gcgataatat aattactgtt tcgtttgtcc tggctgattt
    38821 tgttcagcgt atgtcgccag acttcgtgga taagcccggt ctgtccggca tccgggacag
    38881 gcaatttacc accgccatcc ccgacggcca tcgtggtaat gttgaccttc cgccctcccg
    38941 gtgcggttgc cgctgccagc tttgctgcac cggcagtggt gataacggtt ttgaattttg
    39001 tgctcattat tcctcactta tccggggtaa accgtaatta catcgccgtc gtaagccaca
    39061 ccaccggcga acaggtagcc gggaatgtcc cgggtaatgt tcaggccaat aaggtggcgg
    39121 cttgcaggtt tggcatcagc aatcagccgt tccatttcct gatacattgc ctctgtgatg
    39181 ccactttcca gtacaccaat atcaagccgg aaggtgcccg gcgggtcact ggtttcccac
    39241 cactccgtca cgttgatgag atagcccagc ggctccacca cacgccggat tgcacctata
    39301 gtgcctttat gacagtggat gaaataggca tcgcggataa cagcgcgttt tgtcgcttcc
    39361 ggccactttt catcccatct gtcgaccgaa aacgcccacg ccagccacgg cagcagattt
    39421 gccggacagg agtccgggtt ccacagctca cgaatactga ccggtgtttt ttcaatttcc
    39481 gcacaggctt ttgcggcggc gacctcaagc ggtgatgagc cggtcggcaa cagtcgcgaa
    39541 tcactcatcc gagcctccgg tcacgacgcg gtattcggta cagaaagacg cctgcgtact
    39601 gttgagcacg atgtcggcca gtggtgcggc cagctcgaca cgctgcacgc cttccacatg
    39661 caaagcggca taaatggcag acagacggat gtcgcgcccc agccggtgct gtgccgtgat
    39721 gtacgcttcc agttttttca cggcggcagc gcgtatgggt tcgctttcgg gaccagggta
    39781 aaggtaaagc gtggcgttta tctggtattc aacgatggcg gcagactgca cggtcacgcg
    39841 gtcggccacc ggcctgacgt cctcgccatt aagggcgtta cgcaccaccg ccagcaggtc
    39901 ttcggatgcg acaccgttat tttcacgtga cagcacagag atggtgacgc aggccggaga
    39961 cggactggtg acagagatat ccgcgacacg cccgtcagca ctgcgaccat gatactgata
    40021 ggcacccacc gacccggcga cgcttaaacc ttcaaaggcc tgctgaatac gcagacgata
    40081 atcggtgtca gattccatca cggccggtgt cggcgggatg gtcgaatcat ctgccggggt
    40141 gatagtcaga cgcgtggtgt tgtaattggc accaatcaca tcaaggtcat tacccgcggc
    40201 acaggccagc atcaccgccc gtgcggcctc attcacacgc tgacgccaga gaagctcacg
    40261 ataagcattt tcctccagca gtttgacgag aggctcagat tccagcgtca gggtacgggc
    40321 gaccgcctcc tgctggtctt ccgggtaaag ggaaatcagt gtcgccttgc gttcggcgag
    40381 aatggtttca aagtccagct cctcgaccac atccggtgcg ggtagctggt tcaggtcgat
    40441 aatcggcatg gtttcaactc acagggatgg ttaacgaaag tggctggccg gtgtcgttgt
    40501 gctggccggt taacgtgacc gtcattcgcc cgtcaaaact gcgcgccgtg gtgacagatg
    40561 acagcgtgac gcggggttcc catttcagca ccgccatgta acaggcgacc ttaatctgca
    40621 actcaagcgc cggggtctgc ggctggtcaa tcattgatgc cagcaacgag ccgtaatcac
    40681 gacgcatcac ccgtgagccg accggtgtac gcaggatatc gccgatactc tggctgatat
    40741 gctcaaggtc agtgacagtc aggccattac tgcgattcat tccgagataa cgagctgtca
    40801 taaaggactc ccggttgtgc cgccgctgtc gccggggtgt ttgtgggtat gcagtacctt
    40861 accgtttgat gagagttcac cgccggtgtg ttcaatgttg ccacgcatcg tcccgccctt
    40921 ctgtacttcc agcgtgctag taatcagcct gttggtgcag accacctccg gtgtgtccag
    40981 ggtgatgcga gttgacgctt tcaccatgac caccggcacc gtggcagtaa cagaatcaga
    41041 agccgtcacg ctggccgttt taattccgct taccgtgagt gcactggttt cgggttcata
    41101 ctcaatcacc gccccgtcag ggaaacggat atgcagggca tccgccgacg cagacggcgc
    41161 ggggttatcg ccggaataaa tccccggcag aacgaacgcc gtgtcaagtt caccacccac
    41221 ggccagaatc agcacctgtt cccccacgga aggtgcccac catgtgcgcg aacgcccggc
    41281 gcgatgggtc agccactgaa gccagtcggt gcacatgccg ccggtctgca cacggcagcg
    41341 accggcgtta aggtcggttt cgacgacaag gccggtacga atcatgttgc gcagtgcgcg
    41401 cgcgagttcc tgaatatttg cgagagtgtt catgcgtgtg agattgcaca atatataaaa
    41461 gttatgctat ctggattcat ttgtagaacg accatacaac attcgaggag agcgtaatgt
    41521 tcagtgataa tgtgattaat gcgtggtggt ttatctcttt gtatctattt ttattaatag
    41581 cattaacatt tgttaccttt ggtaaaagta atcttatgag gtttattgca catcatttca
    41641 atcttgagta ttcagacaga aagttaaaaa tgctcgacaa aaaatggcgc gacattcaac
    41701 tatttaaaac aattaacgga atcaatgtat caggcatcga agatgtgaga atgatacagc
    41761 aggggctgat tgatggaaaa ctaaaaacat cgtatttttt ccttactcgc ttctggggtg
    41821 acataacaaa accaccacac ataattaaaa caataattgt aattctggcc agtattattt
    41881 atattctctt cgcatgttat atacacaaca aacaatccgc tatagtaaga gatgccatag
    41941 gcataccata taaaaatatg atgtactatg tttatagtga caaagttctt ttatccttca
    42001 aaaataaaac agttgaactt aataaaactt atagccttgc cgattgcaag accctgcaaa
    42061 acgtatttat aaaagacaca cttcctgaaa tcgcctgcaa taagctctta cagctaaacg
    42121 aggaggactc cgaatggtta agtcaggaga ttaaagataa taacagccac aaaaaagcat
    42181 tattaatact atccctcgtc tatttcattt caggcctgct tatattcctg tcatatacaa
    42241 aattccttta cgccaataag aaggttttag aatacaaagc atcaaataaa aaccactcat
    42301 aaacctctaa acattgagcg accagcacgg ccgctcaatg tttaattgcg catcagcctc
    42361 tgcctggata aaactaacgc tcaaggtgag ccaggatagt ctcttcaatc atctgcacat
    42421 cctcaccggt aaagccgagc aggggacgcg ccggataatc aattttctta ccgtctttcc
    42481 ggttttcttc cgacaggccg aactgatgca cgctggcgat ttttggtgac ttcccgccgt
    42541 aaaattccat tgatgcctgt tccgggctgg cgcggatatg caaaaagcga ctggtgataa
    42601 gtttcgcaaa catttttcgc ttaacacgac cggtcttttt tctggcgctc tgctgctggc
    42661 gtggcgcgta gggtgtgccg tccggggctt tctgtgccat cacccgacgc tgctgactct
    42721 gacgcagacg tttcgccagt tcggtactca gtcgtcgacg ccctgacggt gacagcgact
    42781 caatcagtcc ggtcagccgg tcttcaaaac gcttaaactc attcatccca cttgctcacc
    42841 agttcgccat tgatatacag ctccatcggg cgggtaaccg gctccggcgg cggaggttcc
    42901 gggatattct tcacatgcag cgcgccgtca acctcactga ccagcgtgcg ctcggtcagc
    42961 atcaggctga tgctgatatc aaagctgctg tcattgttga tgtctgcata aaacgtgaag
    43021 ccctttttct ggccttcgtc ggtggtcatg atgtcgggct gattttcccg cagccacgcc
    43081 agcaccggca caatgagcag gtcaaaatca ccggtaaagt cggtcacaat cacattgagc
    43141 gtgtaacgct tttcgaatga catagacgtc gccagtgtgg aggcaatact cccgttatcc
    43201 acgaatatcc gcagcatctc gggactggtt ttcagcaccg tgacggcatc agtcagcgcc
    43261 ctgcgcaggc tgtcgggttt gagcatcgtt ttcgtcctga cagtgtttaa tcatttttac
    43321 ctggctggca cagcgtgcca gcgcgttctc aagcagccgg atatcggcac ttaaatcgcc
    43381 gttcttctgc gggtcactgc ccggcatcgg gcaaagactc actttcgggc aggcgttgtg
    43441 gacaatcact ggcgtctgcg caggccgggc gctggtgcaa ccggcgcaca gcatcaggca
    43501 ggtcagcacc gtaccagcgg cgaaaatctt cgttttcatt gagtaacctc gtgatggttt
    43561 tctcgcgctg tgcttcacgc ttcgcggcgt tctccagttc ctgacgcagt gccacctgcg
    43621 ccagctcgtt tttgtctgcc ctggtgatgg caacatgaag ctgatttttc agcatggtga
    43681 tggtcgtctg ctgttcactg gcgacgttat tcgccctgtc cagcgaggcg cgcaggctgg
    43741 catttttgtg tttcaccaga aacagaccgg ccaccgccag cgataacaac acgaccagca
    43801 caatcatcag ctttgacata gttcccgccc ctcaagacgc tgacgacagg ctttacgtat
    43861 cagccggaaa aacagcgacg ccacaagata aatcagcgcg gtaaaaatcc acccggcagc
    43921 gaccagcgag ataaacgtcg ccaccatcac caccagagcc gccgcccgtc tgcgccacgg
    43981 caccggctgc aaaaacagcg acgtgacaat cttcacggcc agcgattccg gcggcagctc
    44041 ccgcccgtag agttccagca catactccgt ggcatacacg ccgacaccac cggcaaccac
    44101 acagataacc gtcgccagaa tcgcccaggt ggcgacaaaa ctgacggcca cgctctgcgg
    44161 gtaaatcagg gacagtgcca gcatcagcgc cagcgacacg ttcagcatca gtgaaaggga
    44221 taatttcttc atggtgttta ctccgtttaa gccggtacgc cgccggcggt acgccagacg
    44281 gtgaccagtt tttccagtga atgctcacgc tgaccgtaac cggcacccgg cagggacgcc
    44341 cagatattgc gacagcgtga aatggcgcgc tcaatgcgtc ccgcccggat gtcatccagt
    44401 gcaccgcgtt cgcggatcaa ctgaatggcg agcctgtcct gtgacaacgg actgaaatcc
    44461 ggcagggcaa gctgtttgcg gtagtgcggc cagaacaggt aaagctgctg atagcgaccg
    44521 gaggccgtgg atttttcacc gcgacggtta aacaccttcg ccggtcggcc atgcgcgaac
    44581 gggtgatcac tgtagtcggt gaaaatttcc ggctttccgt ccagtccggt gactatcacg
    44641 tcatagcccc ggtttttcgt cagcggatga ttcgccgtcc cttcggacac ggccagcatg
    44701 tcgagaaagg cggcgatatt ctgatgcgtg ttaattaccg gcattacggt ttccccctgc
    44761 ccttaaagcg gcgctgaatg gcaatctcaa tcacctgata accggcgata cccagcatgg
    44821 aaccgatgcc gcacaccgca ggcagtgaca ggtcaggaaa ctgcaccaga acaacaccgg
    44881 caaccatcga gacaaaaccg ccgagcaaca tgcgcccgat aaacagacgc ggggtgatgg
    44941 gttcaccacc ggcaagcacc ttgccgacaa caatcagcac cccaatcatg aaaagcgaca
    45001 ggacgctttt ttcttctgct gtcatgcgtt actcccacag attgacagtt tcagccacgg
    45061 gcgcggtctg aacgtcgggc agttcgacgg cggtgccgtg tggcagcacc gcacccagtt
    45121 cagccagtcc cggatttgcg gcgagcacgg tctcaaccac gccctcagtg cgcccgtaat
    45181 accggacaca aatggcgtcg agcgtgtcgc cctgtagcgc aaaggtcttc atcagatttg
    45241 actcacgatg cagcgcggct tgtcctggat acgcgccacc gcccagcgca tatcccgcca
    45301 cagttcatca atggtgctgt caatgctgtc ggccttcttg tcgcctttcg cactggcatc
    45361 cacgccgcga taacgctcat aaagcgacgc ggtcgccatc gcacacacgg cgcgctcgta
    45421 gtaaaaaact ttgatgcttt caccgtcgat gtcgtccgcc ggaacgtccg ccagacgcgt
    45481 aaaaccggcg gcaattttct gttcgcggta ctcgtacagc tccgcattcg tttcagccat
    45541 gcctgacttg atggcctcac gcagacgggc gggggcgacg gtctgctcaa ggcgcatacg
    45601 ttcccggacg cgcttcgggt cgatatcggg aaaaaagaac gtgtttttaa tcaccggctc
    45661 gtcgcctgcc ggttgcggga tgaccaccgt accctcaccg gacacgggag cctcctttcg
    45721 cggaataatc agcgtcatca tgactacctc tgaaaagtcg ggcggtggac gccggtgcag
    45781 tgtcaggtaa ttcaccgtca ctgaccggcg tgccgccctg gcgcggggcg cattcggttg
    45841 ttaactggct ttctttttcg ggcgtccacg ttttgccggt gtcacgctcc gggtcttacg
    45901 cgggacacgg gtggccgctt tgggctgcgg ctccggcttc ggtttcagct cccgctccag
    45961 tcgttcaatc tcttttttga cgcctgcctg acagtcgagc tgtgtcgcac gttgcaggtg
    46021 agccagcgca ccggcggcat caccaccatc acgcagaaac agaccggtga ttttgtgcag
    46081 ctttgcgcgc acttcatcag gcatgtcagc cgtggcggtc agttcaaggg tctccgtcag
    46141 caggcggata tccacagatt caccggcagc gtgagcgcgc atggccgcga gtgcgacctc
    46201 ctcggtgaac atgtacggcg gggtgcggcg gtgtttaccc ggcatggtca gaccgtactt
    46261 cagggcataa cgggcaatct ccagcgcacc ggcaatatcg ccggtatcca gacgccacag
    46321 catgaccgtc atcagaatgt catcctgtgc acctttgccc tgctccagca caccgttcac
    46381 ccacggcaac cagaacggca gcagctcgcg ctttttcgcg gccttaagct cttttgaata
    46441 aatcgctttc agtgtgcgct ggtctgcggc cagcttaacc agcatctgct catagacagt
    46501 tgcatgtcgc agcggggcgg cttcccgctg cgcggtcatc gctgccgaga cccgcatcat
    46561 gtggcgctgt gcgggactcg tcatcggtta cgctcccggc tctgcggtcg ccttagccgg
    46621 tgtggagaaa tcaccgacct taattttttc caccagacaa ccggcggcgt agtcttccac
    46681 tacgtaatca atgttcattg actcgtagtt ctccacgcgg tcgagtttcg ggttttccac
    46741 aatcacgcgg cgatggctgt catccatgta gtagatggac aggttttcca gcttcgtgat
    46801 gagcatcgca tccgccggga agtacgggac gcgtaccgcc ggcaggttac cgatgcgttt
    46861 ctggctgatg atgacgtcag cggccagcat ttcgctgttg tcctgctcct tgttaacgat
    46921 ggggaaatac ttgtccgcca gtaactgacg tcccacaatc accacaaggt cagggtcttc
    46981 ctgataccac ggctcaatca ggttattggt cgcatccatc accagtgcgt caaggctggc
    47041 ataatcaccg cccttgccca cgcggatgac ctcagaggtg gtgtgccctt cctcgtcagt
    47101 gaccttgctc atcacgcgcg ccggagcttc attgcggtat ttctgcagcc agccgactgc
    47161 cacatcctgc agcatcggat tgctgctgcg gtcagaggtt tcggcacgct tcacgccgtt
    47221 aaaaccggcc atgatgaaat caagggactg gcgtttgata atggcgttac ggatacggag
    47281 ctggaaatcc tgataacgcg cccacaggtc cagcgttttg tagcggatat aaaaatcgaa
    47341 gttaatctgg tcgcattcgt acttgttgga cgccagcttc gagaagtcct tcggctgacg
    47401 ctcggtgcca ccggcggtgt cggtggtgct ggcaatggag ccggtgacac cgatgccaat
    47461 tttttcccct ttcatttcgc tgaccggcac aatgttgatg cgggtcagaa agtcagagga
    47521 ctcctgcatg gtgttcatca gggtctgggt gaccgacggt tcaacggtga attttttcga
    47581 cacatcaccg gcgtcgatgc cgttcagttc ggcaacacgg gacaggtagg cattaaattt
    47641 aaagcgggtt tcctggcgca tagtttttcc tgaaattaag ggttaatcgt gaaggttttc
    47701 ccggactgac tgacgccggt cagcagttcg tcatcagggc gtcaccgcca ccaccggtgg
    47761 ccttgctgcg gcgctgctgg gtcagacttt cggtgtggtc gagactgttt ttcaggcggg
    47821 tgaatgcctg gctggtttca tccgccctgt cagtcacctc ctgcttaagt gcggaaaagg
    47881 cggtttccat ctcagcgagg cgctgctcag tggcgctcag tttttcctgc acatgttcag
    47941 caacagcggt caccgcgtca tgcacgtcat tcagacgggc atcatcgctg gcctgtttgc
    48001 ggccaaaaat ggacttcacc ttttcggtca gggcggtgaa cacggtttca ggcaggtctt
    48061 caaattccag ctcaacaggc gttgccactg aaatcaggtt ttcagggctt aatttgaagc
    48121 ggttcagagg gttgtgtttt gccgtgcggc agaattccag gtattccgtg ccgaggcttg
    48181 ccgggtcatc ggtgacggcc agccccacca gataacattt gccggtgttg gcaaagttcg
    48241 gctgaatttc cattgaggta tagaccttct gcgcggcctt gttcatcgcg ataaggtcat
    48301 cggtcggggt gattttcgca aacagcgccc atttgccttt cagcgccgaa tcatcgtcaa
    48361 tcttttcggc cttcagttcg accacatcac cataacgttt aaaaataccg tcaggcagga
    48421 tgccgcgcag atgttccagg ttaatacggc aaccatagac acgcgggtca aaggtttcgg
    48481 ccatttcctg aatatcctgc gcactgatga cacgcccgtc acaggtgtca ccctcaacgc
    48541 cgatacgaaa gaattttgag actttttttg ccattgtcag gagtcctgaa tagtgattag
    48601 aggagtcaca tgtcggcatc agtttcccga cgatgcgcat cctccgccat cagtcccgga
    48661 tggcttatca ctgacacaac agcaccttag cgaatcgcgg gacgcgactc agtagccttg
    48721 ccgtgtattt atcacggcga ggtattcatg accatcacca cagacaccac tcttttacac
    48781 gacccgcgtc gtcaggcggc gctgctgtac tggcagggat tttccgtgcc gcagattgcc
    48841 gccatgttgc agatgaaacg cccgacggtg cagagctgga aacagcgcga cggctgggac
    48901 agcgttgccc ccatcagccg tgtcgaaatg agtctggaag cgcggctgac ccagctcatc
    48961 atcaaaccgc agaaaaccgg cggggacttc aaggaaattg acctgctggg acgccagatt
    49021 gaacgactgg cacgggtcaa ccgttacagc cagaccggca acgaggcaga ccttaatccg
    49081 aacattgcta accgcaacaa aggtgggcgt cgcaaaccga aaaagaattt tttcagtgac
    49141 gaagccatcg aaaagctgga gcagattttc tttgagcagt ctttcgacta tcagttgcac
    49201 tggtatcgcg ccgggcttga gcaccgcatc cgcgatatcc tgaaatcccg ccagattggc
    49261 gcgacgtttt atttttcccg cgaggcgctg ctgcgcgccc tgaaaaccgg tcataaccag
    49321 atttttctgt cggccagtaa aacgcaggcg tatgtgttcc gcgaatacat catcgccttt
    49381 gcccggctgg ttgacgttga cctgaccggt gacccgattg tcctgggcaa taacggcgca
    49441 aaactgattt ttctcggcac caactccaac accgcacaga gccataacgg cgacctgtac
    49501 gtcgacgaga ttttctggat cccgaatttt caggtactgc gtaaggtggc atcaggtatg
    49561 gcctcacaga gtcacctgcg ttcgacctat ttctccaccc cgtccacgct ggcgcacgac
    49621 gcctacccgt tctggtcggg tgaactgttt aaccggggac gcgccagcgc cgccgaacgc
    49681 gtggaaatcg acgtcagtca taacgccctt gccggtggac ttctctgtgc ggacggccag
    49741 tggcggcaga ttgtcaccat tgaggacgcc ctgaaaggcg gctgcacgct gttcgacatt
    49801 gagcagctca aacgcgaaaa cagcgccgac gattttaaaa acctgttcat gtgtgaattt
    49861 gttgacgaca aggcgtcggt gttcccgttc gaggagctgc aacgctgcat ggtcgacacg
    49921 ctggaagaat gggaagacta tgcgccgttt gccgcgaatc cgttcggctc ccgcccggta
    49981 tggattggtt acgacccgtc acaccgtggc gacagcgccg gatgcgtggt actggcaccg
    50041 ccggtggtgg ccggtggcaa attcagaata cttgagcgtc accagtggaa aggcatggac
    50101 tttgccactc aggctgaatc catccgcaaa ctcaccgaga aatacaacgt cgaatacatc
    50161 ggtattgatg ccaccggcct cggtgtcggc gtgttccagc tcgtgcgctc gttctatccc
    50221 gccgcacgcg acatccgcta cacgccggaa atgaaaaccg caatggtgct caaggcaaaa
    50281 gacgtcatcc gccgtggctg tctggaatat gacgtcagcg ccaccgacat caccagctcg
    50341 tttatggcta tccgcaagac catgaccagc agcggacgca gcgccaccta tgaggccagc
    50401 cgcagcgagg aagccagcca cgccgacctc gcctgggcga ccatgcacgc cctgttaaat
    50461 gagccactca ccgccggtat cagcaccccg ctgacatcca ccattctgga gttttactga
    50521 tgagcaagaa aaaagggaaa acaccgcgac cagcggcaaa aacaatgacc gccagcgccc
    50581 cgaaaatgga ggcattcacc tttggtgagc cggtgccggt actcgaccgc cgtgacattc
    50641 tggattacgt cgagtgcatc agtaacggca gatggtatga gccaccggtc agctttaccg
    50701 gtctggcaaa aagcctgcgt gctgccgtgc atcacagctc accgatttac gtcaaacgca
    50761 atattctggc ctcaacattt atcccgcatc cgtggctttc gcaacaggat ttcagccgct
    50821 ttgtgctgga ttttctggtg ttcggtaatg cgtttctgga aaagcgctac agcaccaccg
    50881 gtaaggtcat cagactggaa acatcaccgg caaaatatac ccgccgtggt gttgaggagg
    50941 atgtttactg gtgggtgccg tccttcaacg agccgacacc tttcgcgccc ggctccgtgt
    51001 ttcacctgct ggagccggat attaatcagg agctgtacgg cctgccggaa tatctcagcg
    51061 cccttaactc tgcctggctg aatgagtcgg ccacgctgtt ccgccgcaag tattacgaaa
    51121 acggcgcgca tgccggatac atcatgtacg tcaccgatgc cgtgcaggat cgcaacgata
    51181 tcgaaatgct tcgcgaaaac atggtgaagt cgaaaggccg caataacttt aaaaatctgt
    51241 ttctctatgc cccacagggg aaagccgacg gcattaaaat tatcccgctc agtgaagtgg
    51301 caacgaagga cgattttttt aatatcaaaa aagccagcgc cgctgacctg ctggacgcgc
    51361 accgcatccc ctttcagttg atgggcggca agccggagaa cgtcgggtcg ctgggtgata
    51421 ttgagaaagt ggcaaaggtc tttgtccgca atgagcttat cccgttacag gacaggatcc
    51481 gcgagataaa cggctggctc ggtcaggagg tcatccgctt taaaaactac tcactggaca
    51541 ctgacaacga ctgaacatcg ccgcctgcag gcggcttttt tacaccccgt catcacgccc
    51601 tcacacgctc accaccgcac aaaacacccc gcagacacac caacgcctca acgggcagac
    51661 taagcgccgt cacgacgcgc tcagacgctg aaaaaataaa atcagcacca ccgccagcgc
    51721 gcagtgcttt ccccgcctcg cccgcccgct tcatgcaggg ctttaaatgc agtgcagcag
    51781 taatggttag aatgcgccag cactgatgtt agataccaga actggcattt aaaaatgaat
    51841 gcaaataaat gcaccaaact aattacttac tgacaaaaaa taagtaaaat caggcgcaag
    51901 ttggcattga gacccctttc agggtattag gaataattat ttcaggagaa aaaatcatca
    51961 cttcctc
//
LOCUS       DAZGAP010000007        51655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGAP010000007 DAZGAP010000000
VERSION     DAZGAP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.007"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(269..1462)
                     /gene="tyrB"
                     /locus_tag="ACHLAM_000360"
     CDS             complement(269..1462)
                     /gene="tyrB"
                     /locus_tag="ACHLAM_000360"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO7851950.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1715..2794)
                     /gene="alr"
                     /locus_tag="ACHLAM_000361"
     CDS             complement(1715..2794)
                     /gene="alr"
                     /locus_tag="ACHLAM_000361"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO7851951.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2847..4262)
                     /gene="dnaB"
                     /locus_tag="ACHLAM_000362"
     CDS             complement(2847..4262)
                     /gene="dnaB"
                     /locus_tag="ACHLAM_000362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO7851952.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4345..5328
                     /gene="qorA"
                     /locus_tag="ACHLAM_000363"
     CDS             4345..5328
                     /gene="qorA"
                     /locus_tag="ACHLAM_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO7851953.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5494..5736)
                     /gene="pspG"
                     /locus_tag="ACHLAM_000364"
     CDS             complement(5494..5736)
                     /gene="pspG"
                     /locus_tag="ACHLAM_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO7851954.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5870..6865)
                     /gene="dusA"
                     /locus_tag="ACHLAM_000365"
     CDS             complement(5870..6865)
                     /gene="dusA"
                     /locus_tag="ACHLAM_000365"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO7851955.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDVDPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            7021..7536
                     /gene="zur"
                     /locus_tag="ACHLAM_000366"
     CDS             7021..7536
                     /gene="zur"
                     /locus_tag="ACHLAM_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO7851956.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRNNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(7578..7787)
                     /gene="yjbJ"
                     /locus_tag="ACHLAM_000367"
     CDS             complement(7578..7787)
                     /gene="yjbJ"
                     /locus_tag="ACHLAM_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO7851957.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(7903..9228)
                     /gene="dinF"
                     /locus_tag="ACHLAM_000368"
     CDS             complement(7903..9228)
                     /gene="dinF"
                     /locus_tag="ACHLAM_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO7851958.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(9301..9909)
                     /gene="lexA"
                     /locus_tag="ACHLAM_000369"
     CDS             complement(9301..9909)
                     /gene="lexA"
                     /locus_tag="ACHLAM_000369"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO7851959.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(10019..10387)
                     /gene="dgkA"
                     /locus_tag="ACHLAM_000370"
     CDS             complement(10019..10387)
                     /gene="dgkA"
                     /locus_tag="ACHLAM_000370"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO7851960.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            10558..12981
                     /gene="plsB"
                     /locus_tag="ACHLAM_000371"
     CDS             10558..12981
                     /gene="plsB"
                     /locus_tag="ACHLAM_000371"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO7851961.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPGVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(13136..14008)
                     /gene="ubiA"
                     /locus_tag="ACHLAM_000372"
     CDS             complement(13136..14008)
                     /gene="ubiA"
                     /locus_tag="ACHLAM_000372"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO7851962.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14021..14518)
                     /gene="ubiC"
                     /locus_tag="ACHLAM_000373"
     CDS             complement(14021..14518)
                     /gene="ubiC"
                     /locus_tag="ACHLAM_000373"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO7851963.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(14697..15617)
                     /gene="malM"
                     /locus_tag="ACHLAM_000374"
     CDS             complement(14697..15617)
                     /gene="malM"
                     /locus_tag="ACHLAM_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO7851964.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(15855..17195)
                     /gene="lamB"
                     /locus_tag="ACHLAM_000375"
     CDS             complement(15855..17195)
                     /gene="lamB"
                     /locus_tag="ACHLAM_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO7851965.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(17267..18382)
                     /gene="malK"
                     /locus_tag="ACHLAM_000376"
     CDS             complement(17267..18382)
                     /gene="malK"
                     /locus_tag="ACHLAM_000376"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO7851966.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            18747..19937
                     /gene="malE"
                     /locus_tag="ACHLAM_000377"
     CDS             18747..19937
                     /gene="malE"
                     /locus_tag="ACHLAM_000377"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO7851967.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            20091..21635
                     /gene="malF"
                     /locus_tag="ACHLAM_000378"
     CDS             20091..21635
                     /gene="malF"
                     /locus_tag="ACHLAM_000378"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO7851968.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            21650..22540
                     /gene="malG"
                     /locus_tag="ACHLAM_000379"
     CDS             21650..22540
                     /gene="malG"
                     /locus_tag="ACHLAM_000379"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO7851969.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(22634..23044)
                     /gene="psiE"
                     /locus_tag="ACHLAM_000380"
     CDS             complement(22634..23044)
                     /gene="psiE"
                     /locus_tag="ACHLAM_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO7851970.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            23258..23536
                     /locus_tag="ACHLAM_000381"
     CDS             23258..23536
                     /locus_tag="ACHLAM_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7851971.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(23583..25679)
                     /gene="yjbH"
                     /locus_tag="ACHLAM_000382"
     CDS             complement(23583..25679)
                     /gene="yjbH"
                     /locus_tag="ACHLAM_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7851972.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(25679..26416)
                     /gene="yjbG"
                     /locus_tag="ACHLAM_000383"
     CDS             complement(25679..26416)
                     /gene="yjbG"
                     /locus_tag="ACHLAM_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7851973.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLARLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(26413..27051)
                     /gene="yjbF"
                     /locus_tag="ACHLAM_000384"
     CDS             complement(26413..27051)
                     /gene="yjbF"
                     /locus_tag="ACHLAM_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO7851974.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWIEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(27238..27480)
                     /gene="yjbE"
                     /locus_tag="ACHLAM_000385"
     CDS             complement(27238..27480)
                     /gene="yjbE"
                     /locus_tag="ACHLAM_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO7851975.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            complement(27978..29627)
                     /gene="pgi"
                     /locus_tag="ACHLAM_000386"
     CDS             complement(27978..29627)
                     /gene="pgi"
                     /locus_tag="ACHLAM_000386"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO7851976.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      29864..30060
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            30152..31501
                     /gene="lysC"
                     /locus_tag="ACHLAM_000387"
     CDS             30152..31501
                     /gene="lysC"
                     /locus_tag="ACHLAM_000387"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO7851977.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            31754..32026
                     /gene="yjbD"
                     /locus_tag="ACHLAM_000388"
     CDS             31754..32026
                     /gene="yjbD"
                     /locus_tag="ACHLAM_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO7851978.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(32027..32899)
                     /gene="rluF"
                     /locus_tag="ACHLAM_000389"
     CDS             complement(32027..32899)
                     /gene="rluF"
                     /locus_tag="ACHLAM_000389"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO7851979.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            33247..34194
                     /locus_tag="ACHLAM_000390"
     CDS             33247..34194
                     /locus_tag="ACHLAM_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO7851980.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVNALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            34264..35070
                     /locus_tag="ACHLAM_000391"
     CDS             34264..35070
                     /locus_tag="ACHLAM_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7851981.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTRLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            35080..35487
                     /locus_tag="ACHLAM_000392"
     CDS             35080..35487
                     /locus_tag="ACHLAM_000392"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO7851982.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            35487..35981
                     /locus_tag="ACHLAM_000393"
     CDS             35487..35981
                     /locus_tag="ACHLAM_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO7851983.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            36047..36844
                     /locus_tag="ACHLAM_000394"
     CDS             36047..36844
                     /locus_tag="ACHLAM_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO7851984.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            36855..37679
                     /locus_tag="ACHLAM_000395"
     CDS             36855..37679
                     /locus_tag="ACHLAM_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO7851985.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLLVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            37731..38960
                     /gene="sorE"
                     /locus_tag="ACHLAM_000396"
     CDS             37731..38960
                     /gene="sorE"
                     /locus_tag="ACHLAM_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO7851986.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            39342..40031
                     /gene="pepE"
                     /locus_tag="ACHLAM_000397"
     CDS             39342..40031
                     /gene="pepE"
                     /locus_tag="ACHLAM_000397"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO7851987.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(40121..41752)
                     /gene="yjbB"
                     /locus_tag="ACHLAM_000398"
     CDS             complement(40121..41752)
                     /gene="yjbB"
                     /locus_tag="ACHLAM_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO7851988.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(41972..45655)
                     /gene="metH"
                     /locus_tag="ACHLAM_000399"
     CDS             complement(41972..45655)
                     /gene="metH"
                     /locus_tag="ACHLAM_000399"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO7851989.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMEFLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLSGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            45855..46679
                     /gene="iclR"
                     /locus_tag="ACHLAM_000400"
     CDS             45855..46679
                     /gene="iclR"
                     /locus_tag="ACHLAM_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO7851990.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(46696..48420)
                     /gene="aceK"
                     /locus_tag="ACHLAM_000401"
     CDS             complement(46696..48420)
                     /gene="aceK"
                     /locus_tag="ACHLAM_000401"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO7851991.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGNLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(48636..49940)
                     /gene="aceA"
                     /locus_tag="ACHLAM_000402"
     CDS             complement(48636..49940)
                     /gene="aceA"
                     /locus_tag="ACHLAM_000402"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO7851992.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(49970..51571)
                     /gene="aceB"
                     /locus_tag="ACHLAM_000403"
     CDS             complement(49970..51571)
                     /gene="aceB"
                     /locus_tag="ACHLAM_000403"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO7851993.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT        12344 a        13798 c        13284 g        12229 t
ORIGIN      
        1 tggatataga aaaattctat cgtaacaaac ccgatactta ttattaaatt taataatggt
       61 tagatgtgca aaaattatta ttccacacgg taaaacaggg aaaagtactc cagaacattg
      121 ggataaactt aacaagttag aactaacaac gttttattac ctctcacgta gaacgatggc
      181 atcaaaaaaa tcatctataa agcaagagtg aaagcgacga cagtttaatt tcactgcagg
      241 ctggataact ccagcctgct ttcctgcatt acatcaccgc agcaaacgcc tttgccacac
      301 gttgtacatt tgccgtattt aacccggcga cacacatgcg accgctggcg atgagataga
      361 caccaaattc ttcacgtagt cggtcaacct gagcggcact taaaccggta taactgaaca
      421 taccgcgctg attaagcaga taatcgaaat tgcgttctgg tatctctgtg ctcaatacct
      481 tcaccagttc ctgacgcatc gccagaatgc gagtacgcat ctcttctact tccgccagcc
      541 agctggcttt caatgcctcg tcattcagca ccgcagccac cacctgcgca ccaaaattcg
      601 gcgggctgga gtagttgcgg cgaactgttg ctttcaattg ccccagtacg cggcctgcgg
      661 cttcggcatc ttcacacaga acagaaagtc cgccgacgcg ctcgccgtaa agggagaaaa
      721 ttttcgagaa cgaattgctc accagagcgg gtaatccagc gctggcaatg gcgcgaatgg
      781 cgtaggcatc ctcttccata ccggcaccaa atccttgata ggcaatatcg aggaatggaa
      841 taagctcgcg ggctttgaga atttcaatca cggaatccca ctggtcatta gtgagatcgg
      901 cacccgttgg gttgtggcaa catggatgca gcaacacaat actgcgggca ggtaatgttt
      961 tcagcgtcgc caacaggtca ttaaagcgca cgccgttagt cgcttcgtca taccaggggt
     1021 aagtgctcac ttcgaatcca gccccggcga atattgctac gtggttttcc caggtaggat
     1081 cgctgaccca gacgcctgat tccgggaagt agcgtttcag gaaatctgca cccactttca
     1141 atgcccctga gccgccaagg gtttgaatgg ttgctacgcg ctgttgttgc agtaccggat
     1201 ggtccgcacc aaacagcagc ggcgcaatgg catggcgata gctgttaagc ccttccatcg
     1261 gtaaataaag cgaagcacca tgaggctgcg cattcaggcg cgcttccgca tccgctacgg
     1321 ctttcagttg tggaataatt ccgtcttcgt tgtagtacag accgatactt aaattcactt
     1381 tgtcgctgcg agggtcttct ttaaaacgct ccataagcgt aagaatcggg tcgccagcgt
     1441 aggcgtcaac tttttgaaac acgcgatggt tctccaggtt tacgggcagg tggttaaaac
     1501 acaataaacc ggaagaaggc gaagatcgag aggatgttca ggggcgaatg gcaattagca
     1561 acagagtgag actcatgaca aacgtacatc cgccagatgc acgaccttta taagagcatg
     1621 aaagaatatc aacttattga attggtaaga ttttattggc cggataaggc attcacgccg
     1681 catccggcac agacaatcaa atattacaga acgattaatc cacgtatttc atcgcgaccc
     1741 ttgaagtcag gcgcgtaata agttcgtaag cgctcacttt tgtcatttca gcgatacgtt
     1801 ctacgggcaa accttcgccc cataaaatga ccgggtcccc ggctttgtcc tgcgcctgtg
     1861 gacctaagtc tacgcagatc atatccatcg cgacgcgccc gacaatcggc acttcgcgac
     1921 cgttcaccag cactggcgta ccggacggcg cggcgcgcgg ataaccatcg ccatagccca
     1981 tcgcgactac gccaaggcgg gtatcacgtt cgcttaccca ggttccacca taaccgacag
     2041 gctctccggc tttatgctca cgcacagcaa tcaggctgga ggtcaatgac attaccggct
     2101 gacagccaaa atcggcaccg gtggaacgat cttccagcgg cgagacgcca taaagaatga
     2161 tgcccgggcg cacccagtca aaatgcgact gtggccacag cagaatgcca cccgacgcgg
     2221 caatggaacg ttgacctggt ttgccttcac aaaaggtatt aaagatagcg agttgtttct
     2281 cggttgcgcc gcattttggt tcatccgcgc gcgcaaaatg gctgacgata ttcaccggct
     2341 gacgaacgtt tttgcactgg gtcaggcgat gataaaacgc ctcagcctgt tccggcctta
     2401 cgcccagacg gtgcataccg gtatcgagtt tcatccagac ggtaaccggc tcgtccaggc
     2461 tagcctcttc cagcgcagcc agctgttctt cgttatgcac ggcggtatga aaatgttgcg
     2521 cagaaatcgt cggcagatct ctggcatcaa aaaagccttc gagtaacagt acaggtttgg
     2581 tgattccccc cgcacgcagt cgcagagctt cttcgagacg ggctacgcca aaggcgtcag
     2641 catcggggag cgttcgcgcg gtctcaagaa gaccgtgacc ataagcgttc gctttcacca
     2701 ccgcaaccat tttactggca ggagccagtt cacgaagacg ttgcaggttg tgtcgcagag
     2761 cgcggcggtt aatcacaaca gttgccgctt gcatttgtgt tccttgataa gtgtttgctt
     2821 taattaccta attcataaaa taattattat tcgtcgtcgt actgcggccc cgcatagttg
     2881 tcgaagcgcg accattgacc gttaaaggtc aggcgtaccg tcccgattgg gccgttacgt
     2941 tgtttaccga taataatttc cgcgatgcct tttaaatcac tgttttcgtg atacacctca
     3001 tcacgataga taaacatgat caagtccgca tcctgctcga tagagccaga ttcacgcagg
     3061 tcggagttga ccgggcgttt gtcggcacgt tgttccagag aacggttcaa ctgggacagc
     3121 gccaccaccg gcacgttcaa ttctttcgcc agtgctttca gcgagcggga gatttctgca
     3181 atttccagcg tacggttatc ggaaagcgcc ggtacgcgca tcagttgcag gtagtcgatc
     3241 atgataagcc cgatgccgcc gtgttcacgg gcaatacggc gcgcgcggga acgcacttcc
     3301 gttggcgtca ggccggagga gtcatcgata tagatattgc gtttttcgag caaaataccc
     3361 atggtgccgg aaatgcgcgc ccagtcttca tcatcgagct gcccggtacg gattttagtc
     3421 tggtcaacgc gcgacagcga cgccagagaa cgcatcataa tctgttctga tggcatctcc
     3481 agcgagaaga taagtactgg tttatcctgc aacatcgccg cgttttcgac gaggttcatc
     3541 gcaaatgttg ttttacccat cgacggacgc gcggcgacga tgatcaaatc cgacggctgc
     3601 aagccagcgg tttttttgtt gagatcgtca taaccggtgt ttaccccggt aacgccatcg
     3661 tgtggctgct gaaacaactg ctcaatacgc gccaccgtgg cgtcgagcac atcagcgatg
     3721 ttcttcggcc cttcgtcttt gtttgcacga ctttcggcaa ttttaaagac gcgggattca
     3781 gccaggtcca gcagatcttc gctggtgcgc ccctgcggat caaaaccggc ttcggcaatc
     3841 tcattcgcaa ccgaaatcat ctcgcgaaca acggcacgtt cacgcacgat gtcagcataa
     3901 gcactgatgt tcgccgcact tggcgtattt tttgacagct ctgccagata agcaaaacca
     3961 ccgacgctat cgagttgccc ctggcgttcc agcgattccg caagggtaat cagatcgata
     4021 ggactaccgc tttcctgcaa acgcgccatt tcagtaaaga tatgacggtg tgggcgggtg
     4081 taaaaatcat ctgccaccac acgctcagct acatcatccc agcgttcgtt atccagcatt
     4141 aaaccgccca acaccgactg ctccgcttcg atcgagtgcg gaggcacttt cagcccggca
     4201 acttgtggat cgcgttcgcg gggttcagcc tgctgtttgt tgaagggttt atttcctgcc
     4261 atagtgaatg gagttaccga gataaagaat gggtcgaaac tttaccatat gaagcagacc
     4321 ctgacgatac gttctggagg acacatggca acacgaattg aatttcacaa gcacggtggc
     4381 ccggaagtac ttcaagccgt agagttcact cctgccgatc cggcggagaa tgaaatccag
     4441 gtcgaaaata aagccatcgg catcaatttt atcgacacgt atatccgcag cggcctttac
     4501 ccgccgccat cgctacccag cggattaggc accgaagcgg caggcatcgt gagtaaagtc
     4561 ggcagtggtg taaagcatat taaggcaggc gatcgcgtag tctatgcaca atcggcatta
     4621 ggcgcttaca gctctgtgca taacattaat gcggataaag cggcgattct gcctgcggca
     4681 atttcttttg agcaagctgc ggcatccttc ctgaaaggct taacggttta ttatctgctg
     4741 cgcaaaacct atgaaattaa acccgatgag cagttcctgt tccacgcagc ggctggcggc
     4801 gttggcttga ttgcctgcca gtgggcaaaa gcccttggcg cgaaacttat cggcaccgta
     4861 ggaaccgcgc aaaaagcgca gagtgcgcta aaagcgggcg catggcaggt tattaactat
     4921 cgtgaagaga atctggtcga gcggttaaaa gagatcaccg gtggcaagaa agtgcgcgtg
     4981 gtgtacgatt ccgtgggcag agacacctgg gaacggtcgc tggattgcct gcaacgccgc
     5041 ggcttaatgg tgagctttgg caactcatca ggtgcggtta ccggtgtgaa cttaggcatt
     5101 ctcaatcaga aaggctcgtt gtatgtgaca cgcccttccc tacaaggcta tatcaccaca
     5161 cgggaggaat taaccgaggc cagtaatgaa ctgttctctt tgattgccag cggtgtgatt
     5221 aaggtcgatg tcgccgagca gcagaaatat ccgcttaagg atgcacagcg tgcgcatgag
     5281 attctggaaa gccgggcgac gcaaggttcc agcctgttga ttccataaaa gaaatagggc
     5341 ttccatctgg gaagcccttt ctttttatag ttcggctgta tgtagggtac agcacgatga
     5401 atctgttaga ggcgcaatag tgacagattt gattatcaat tcctattttg ttctaaggat
     5461 aaaaccttag gttgtgatca tccgcacaat cccttagtaa cgccagcggt cataacgctg
     5521 atatttcggc acttttggtg ctttaatcgc cttaataacc cacaccaccg caatcgccag
     5581 gagtaaccac ggcagcaact taatcatcaa tgccagcata ccgccgagga acataatggc
     5641 cgtcgccaca accagcgcgg cgataatgcc cagcaacgaa acgccggtga ccatcagcat
     5701 gacaaaaaag ccaatcacaa aaagtagttc cagcatgatg ctctcccaaa tatgaaatct
     5761 cttgcaggca ttacaagaat catgccaaaa ataatctatt gatttaacag caaaacgccc
     5821 cgcgacggtg cgcagggcgt ggtgaatttg actacttttt ggtgaaaagt taacgcttat
     5881 ccgccaccag tttgagcgcg tgttccagca cgttaatgtc agcacccgct ttatgggcat
     5941 tttcacttaa ataacgccgc cactgccgcg cgccaggaat accctggaac aagcccagca
     6001 tatgccgggt aatatggccg agatacgtcc cctggctgag ttcacgctca atgtacggat
     6061 acatggcacg caccaccgcc accggatcga catcggtatc cgaagaacca aagatctctc
     6121 ggtctaccgc cgccagaatg cctggattct gatacgcttc gcgcccgacc atcacgccat
     6181 ccatatgttg caaatgcgct ttagcctctt ccagcgactt gatgccaccg ttaatcgaca
     6241 tcgtcagatg cggaaagtca cgcttcagtt gatacacacg cggataatcg agcggcggga
     6301 tttcacggtt ttctttcggg cttaaccccg aaagccaggc tttacgtgcg tggatgatga
     6361 acatctcaca ctcacctttg ccggaaacgg tgttgatgaa atcgcagaga aattcatagc
     6421 tgtcctgatc atcgatgcca atacgcgttt tcaccgtcac cggaatcgac accacatcgc
     6481 gcatcgcttt cacgcagtcg gcaaccagct gcgcattacc catcagacac gcaccaaaca
     6541 tgccgttctg cacccggtca gacgggcagc cgacattcag gttgatctca tcatatccgc
     6601 gcgcttctgc cagcttcgca cactgtgcca gcgccgccgg atcgctaccc ccgagttgca
     6661 acgctaccgg atgttcttct tcactgtacg ccaggtaatc acctttaccg tgaataatcg
     6721 cccctgtagt caccatttcg gtatacagca acgtattgcg ggaaagcaga cgcaagaaat
     6781 agcggcaatg tctgtccgtc cagtcaagca tcggggcaat cgagaaacgg taagcgggat
     6841 aaacagtgtg tgattctgct ggcatggcga ctttttaagc atctggtaaa ttgggggcgc
     6901 tactatagca taacgaatat gcgatcaggc aatgtgacag ccatgacacc ctgttctcgg
     6961 cgtttaagcg agaactatgg taaagtaagg acattcttaa ccccactgtc gaggtgccca
     7021 atggaaaaga ccacaacgca ggagttatta gcgcaggctg aaaaaatctg cgcacagcgt
     7081 aatgtgcgcc tgaccccaca gcgcctggaa gtattgcgcc tgatgagtct gcaggatggc
     7141 gctatcagcg cttatgatct gcttgatttg ctgcgcgaag ctgaaccgca agccaagccg
     7201 ccaacggttt atcgcgcgct ggagtttctg cttgaacaag gttttgtgca taaggtggaa
     7261 tccaccaaca gttatgtgct ctgtcatctg ttcgatcagc ccacccatac ttcagccatg
     7321 tttatttgcg atcgctgcgg cgcggtgaaa gaagaatgtg ctgaaggcgt ggaagacatc
     7381 atgcatacgc tggcggcaaa aatgggtttt gccctgcgga ataatgttat tgaagcacat
     7441 ggattatgtt cagcatgtgt agaagtagaa gcgtgtcgtc atcccgaaca gtgccatcat
     7501 gaccactcta ttcaggtgaa aaagaaacct cgctaaaagg gtgtacatcc ttgtacatgt
     7561 cgggcaggag ggattaatta ccagcggtaa tcatggcgtt tttcccaact atcaacctct
     7621 ttttctgcct gatctttctg ataaccataa cgttcctgga ttttaccgac cagttgatca
     7681 cgtttacctt caatgatcgt catatcatca tcggtcagtt tgccccattg ctctttcact
     7741 ttacctttaa actgtttcca gttaccgccg gcttcatctt tattcataat caagacctca
     7801 tcgttaggtt gtgaatgaga gtacgttcac ttttcttctg aacgtgagat taagtgtagt
     7861 cagcaatttg gcttaggatt atttattcag aatttttaac cgtcacgttg cggcaaacca
     7921 ggtaccgttg cgccagtgac gccgccagat agccgccaga gaaagcccgc gcaaagccag
     7981 aaagacggtt aatgccagcc acaaaccatg attccccagc cacggcagcg taaggagcgt
     8041 cagcgcaaaa cctgcggcgg ccaccgccat actgttacgc atttcggcgg cgcgcgttgc
     8101 acctataaac atgccgtcga gcagataaca ccagacgccg accaacggca aaatcacctg
     8161 ccagataaga tagcggtcag ccagctgctg aatctgggtt aacgacgtca gcaacgcaat
     8221 gatgtgttcc cctgccagca aataaaccac cgaaaacagt aacgctacga tccccgactg
     8281 gcggcacgct gcccgccaga catccagtaa ctggctacca tcgcgcgcac cgtacgcctg
     8341 accggaatgc gcttcaaccg cgtaggcaaa accatccagc gcataggcgg taaaggtgag
     8401 taacgtcatc agaaccgcgt taacagcgat aatgtcactc cccagtcgcg cgccaagtac
     8461 ggtgatcgcg ccgaaacaga gttgcaacaa cagcgagcgc agcatgatat cgcggttaag
     8521 cgccagcaag tgacggaagt ttcctcgcca ggcagttttc agcatttcgc cggagattcc
     8581 gcgtagtttg aggattttac gcaccattag cagaccaatc agcaatgttg catattccgc
     8641 aataaccgtc gccagcgccg cgccctgcac gttcatatgc agccccatca ccagccagac
     8701 atccagcaca atgttgagga tattgccgac cactaacaaa attactggcg cacgggcata
     8761 ttgcacaccg agtaaccaac caagtaatac cagattcgcc agcgacgccg gtgcacttaa
     8821 ccagcggatt tcaagaaagc gtcgcgcctg ttctagcacc gcttcgctgc cgccaacaat
     8881 atgcagcgcc agatcgataa tcggcgtacg cagcagcgca attaacgccc cagcccccaa
     8941 cgccaacagc aacggttgca ccagcgcacg ggctaatgcc tgaggatttt tggcaccata
     9001 agcctgcgca gtcagcccgg tggtgctcat gcgtaaaaac agcaacagca taaagagaaa
     9061 gctggtcgcc gttgcaccaa ccgccacgcc gcccaaataa accggactat caagatgacc
     9121 aattaccgcc gtatcgacca gtcccagcaa cggaacggtg atattggaga aaatcatggg
     9181 taaggcgaga tgccagagtg ctttatcaga tgaagtgagg aatgccatgc agacaagcct
     9241 gatgaagaga gatgaaaaac aaaccgcgac gccaggcggc atcgcggtct cagagatatg
     9301 ttacagccag tcgccgttgc gaataactcc aaccgccagc ccttcaatgg tgaagctctg
     9361 ctgacgaagg tcaacgacaa ttggtttaaa ctcgctattt tctggcaaca gttcgacttt
     9421 attgccctgt tttttcaggc gcttaacggt aacctcgtca tcaatacgtg cgacaacgac
     9481 ctgaccgtta cgtacatcct gagttttatg cactgccagc aagtcgccat ccataatgcc
     9541 gatatctttc atcgacatcc cgctgacgcg cagcaggaaa tcagcattcg gcttgaataa
     9601 ggaaggatcg acctgataat gaccttcaat atgctgttgc gccagaagcg gttcaccggc
     9661 agccacacga cctaccagcg gcaacccttc ttcctcttcc tgcaacagac gaatcccgcg
     9721 tgatgcgccg gaaacaattt caataacgcc tttgcgtgcc agcgccttca gatgttcttc
     9781 agccgcgttt ggggaacgga accccaaacg ctgcgcgatt tccgcacgcg tcggcggcat
     9841 acctgtctgg ctgatgtgat cacggatgag atcaaacacc tcttgttgcc tggccgttaa
     9901 cgctttcatt ccgccccctg ggtgtatata cagttatgct gtgagtatat acagcaaaag
     9961 gcgattttgg aaccataaac tgcacaataa accagagatt tatcgaaatc tggaagggtt
    10021 atccaaaatg cgaccataac aggatgcacc aggtaatcac ggcgacgata atcgcaatca
    10081 gaaccgcggc ggatcccata tctttagcgc gtccggaaag ctcatggtat tcagagccaa
    10141 ttcggtcaac cactgcttcg atggcgctat tgaggatttc cacaatcatc accagcatca
    10201 cggagctgat aagcagcacg cgggtaattg cgtccacatc cagccagcag gcgatgacca
    10261 ccgccaacaa taccgctacg ccttcctgac ggaatgccgc ttcgttgatc catgcagcgc
    10321 gtaaaccttt ccaggaatag ccagcagctt tgataattcg ggtgaatcca gtggtattat
    10381 tggccattga aagaaccttt ttacattatg agcgtcaata tcagtgtacc gttaaatccg
    10441 atgattcggg tttgcgtaac gccgcgcgaa acatgagcgg ataccacaga atttcccatg
    10501 actttctgct atccttgccg cgcatttgca ttattaacca gaggctttac atcgtttatg
    10561 tccggctggc cacgaattta ctacaaatta ctgaatttac cattaagcat cctggtaaaa
    10621 agcaagtcta ttccggcaga tcctgccccg gaactggggc tggatacctc tcgtccaatt
    10681 atgtacgttt taccgtacaa ctcgaaagct gacttgctga cgttgcgcgc ccagtgtctg
    10741 gcacatgacc tgcctgaccc gttagagccg ctggaaatcg acggcacgct actgccgcgc
    10801 tatgtgttca ttcacggcgg gccgcgtgtg ttcacctatt acacgccgaa agaagagtct
    10861 attaagctgt tccacgacta tctcgatttg caccgtagca acccaaatct ggatgtgcag
    10921 atggtgccag tatcggtgat gtttggtcgc gcgccggggc gtgaaaaagg cgaagtgaac
    10981 ccgccgctgc gtatgcttaa cggcgtacag aaatttttcg ctgtactgtg gctcggtcgc
    11041 gacagttttg tgcgtttctc gccgtcagtt tcgctgcgcc gtatggcgga tgaacacggc
    11101 acggataaaa ctatcgcgca gaaactggcg cgcgtggcgc gtatgcactt tgcccgtcaa
    11161 cgtctggctg ccgtaggccc acgtctgcct gctcgtcagg atctgtttaa taagctgctc
    11221 gcctcccgcg ccattgccaa agcggtagaa gatgaagcgc gcagcaaaaa aatctcccat
    11281 gaaaaagcgc agcagaacgc gattgcgctg atggaagaga ttgcggcgaa tttctcttac
    11341 gagatgatcc gcctgactga ccgtattctg ggcttcacct ggaaccgcct ttaccagggc
    11401 atcaacgtcc ataacgccga gcgcgttcgt cagctggccc acgacggtca tgagctggtg
    11461 tatgtgcctt gccaccgcag ccacatggac tacctgctgc tttcttacgt gctgtatcac
    11521 caggggctgg tgccgccgca tatcgccgcc gggatcaacc tgaatttctg gcctgccggg
    11581 ccgattttcc gccgtctggg ggcgttcttt attcgccgta cgtttaaagg caataagctc
    11641 tattccaccg ttttccgtga gtatctcggc gaactgttca gccgtggtta ttccgtcgag
    11701 tatttcgtgg aaggcggtcg ttcccgtaca gggcgtttgc tggatccgaa aaccggtacg
    11761 ctgtcgatga ccattcaggc gatgctgcgt ggcggcacgc gtccgattac gctgattccg
    11821 atctatatcg gttatgagca cgtcatggaa gtgggtactt acgccaaaga actgcgcggt
    11881 gcgacgaaag agaaagagag cctgccgcag atgctgcgcg gtttaagcaa gctgcgtaat
    11941 ctcggtcagg gttacgtcaa cttcggtgaa ccaatgccgt tgatgaccta ccttaaccag
    12001 catgtacctg actggcgtga atctatcgat cccatcgaag cggtgcgtcc ggcatggtta
    12061 acgccgacgg tcaataatat tgctgccgat ctgatggtac gcattaacaa cgcaggcgcg
    12121 gcaaacgcca tgaacctgtg ttgtactgcg cttctggcgt cacgtcagcg ctcactcacc
    12181 cgcgagcagt taaccgagca actcaactgc tacctggatc tgatgcgcaa cgtgccttac
    12241 tccacggact ctaccgttcc ttcagccagc gccagcgagc ttatcgatca cgcgctgcaa
    12301 atgaacaagt ttgaagtcga gaaagacacg atcggcgaca tcatcattct gccgcgcgag
    12361 caagcggtgc tgatgaccta ctatcgcaac aacattgcgc atatgttggt gctgccttcg
    12421 ctgatggcgg caattgtcac ccagcatcgc cacatctccc gcgacgtatt gatggagcac
    12481 gtcaatgtgc tttacccaat gctgaaagcg gagctgttcc tgcgctggga tcgcgacgag
    12541 ttgccgggcg ttattgatgc actggcaaat gagatgcaac gtcaggggct gattaccctg
    12601 caagatgatg agttgcatat caacccggcg cattctcgca cgctacagct gctggccgca
    12661 ggcgcgcgcg aaacgctgca acgttatgcc atcaccttct ggctgttgag cgccaacccg
    12721 tcgatcaacc gcggtacgct ggagaaagag agccgcaccg tcgcgcaacg tctctccgtg
    12781 ctgcacggca tcaacgcgcc ggagttcttc gacaaggcgg tgttcagttc tctggtgctg
    12841 acactgcgtg atgaagggta tatcagcgat agcggcgacg ccgaaccggc agaaacgatg
    12901 aaggtgtatc agttgctggc ggagttgatt acatcagacg tgcgtttgac gattgagagt
    12961 gcgacgcagg gcgaagggta atcagggaga attgccggat gcggcgcaaa cgccttattc
    13021 ggcctaccat gatctgcaaa ttcaataaat tgcgatatgt tgtgtaggcc tgagaagcgc
    13081 agcgcatcag gcaacccaga aggaagccgg atgatcatcc ggctttttta catcatcaga
    13141 aatgccagta actcattgcc agccctaaaa acagtaccag accaacatag ttattattca
    13201 taaatgcttt aaagcaggct tcacgctcgc ggttggcaat cagtttttgt tgataaacaa
    13261 acagcgcgcc agccaccaga attgaccagt aatatcccca gcctaagcca tttaactcac
    13321 cgataatcgc catcagcgcc agtacgccaa tctgcaaaat accaataatc aatttatcgt
    13381 attggccgaa caggattgcg gtggatttaa tgccaatctt cacatcatca tcacggtcaa
    13441 ccatcgcata ctgcgtgtcg tatgccaccg cccagagaat attggcgagg aacattaacc
    13501 agcaactcaa tggcaccgac tcactcacag cagcaaaagc cattggaatc gaccagccaa
    13561 acgccgcgcc cagcaccact tgcggtagat gggtataccg cttcataaac gggtataccc
    13621 acgccagcgc cagcgcggca atcgacaaca gaatagtcat cgtattcagc gtcagcacca
    13681 gtaaaaatga aatcagtacc agcactacaa acagcgcgcg cgcctctttc tccgttaccg
    13741 cgccgctggg gagtggtcgg ttcgccgtgc gcttaacatg accatcaaac ttgcggtcag
    13801 cataatcatt caccacgcaa ccagcagcgc gcatcagcca gacgcccgcg acaaacaccg
    13861 ccaggatcca gagctgggga acgcccggtg tcgccaccca caacgcccat aatgttggcc
    13921 agagcagcag taacgcgcca attggcttat ccgtacgcat taagcgatga aacgccagca
    13981 gcttattctg cgtcagactc cactccatat ttttttcctc ttagtacaac ggtgacgccg
    14041 gtaaaaacag ttctgttagc aacagcggtt taccgcttaa tcgcaggcgg gaacgtcgcc
    14101 cccacagtcc ggcatcacgg cctatctcaa taaagtcccg ggttaatgtc gatgatgtga
    14161 acagatagcg tcctaacggc gttttaccta atttttgtaa cgccagctcc ggcccgctta
    14221 acgttgacac aggaacgacg gtacgaccgg caagccacgg ttcaccatcg gcacataaca
    14281 aaatttcacg taaccagtaa cgagactctt tcggcagcag tggcagttct tcggggattt
    14341 cattctgctc gacaaaccct tcgcggatca tcgtcacgct caccgttttt ccctgctgtt
    14401 caaaacgttt tgtcatggaa tcttccagca acagccagtc gagcagttgc ggctccaggg
    14461 cagggatctc tgtaaaatag cgcagcgcac gcagttgcgt taacgcgggg tgtgacatgc
    14521 cgaactctcc gttacataac gtaaaggcat tgtatcgcag aaaaatattt acgggagatg
    14581 taaagcaaat taagtgaact aatgcgcaac aaaaacgcaa caatagtaca ggaggagaaa
    14641 aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca gcactgtggg gcgtaattac
    14701 cccttgcctt ttacactgct gataaaggtg gaacgggcag atgtcgaccc caggcgttca
    14761 gcttcatcaa gcagttttaa cgccttatca acatcacctt tcgcgacagc gtttttgatc
    14821 gcggtattaa aataactttc cgtgtcgttg agcatcggct cgcttttctt caccggtgcc
    14881 ggagcgggtg cagccacagc tggtgccgcc gtgttgccta ccgtaaccgg agctggagcg
    14941 gaagaaccaa atagaggccc gaccaacacg ctagaactgg agttcgtttt gactttcagt
    15001 ttcagtaagc catcggtggt atgacgagca accgggtcag gaatatccgg gatcgagtta
    15061 ccgacgcctt tggcataggc tttagccggg tcgagcagtt gggtcgtctg ctggaggtct
    15121 ttttccgtgg taaagaccag aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc
    15181 ataacgcctt ccagacgatc tgcactcatc acgcctggtt cctggtaggt gaaataactg
    15241 ctggggaaga aggctgatgg ggtcatgttc tgatcaagaa tcagcacgtt cggcgcaaat
    15301 acgctagttt gtttgttcac ttcgctggtc agcgttaggg tcagttcgcc aatgtttgcc
    15361 gggacgctgt acgcagcaac cggacctctg atgccgggaa cgttcagttg ttggccgcca
    15421 gtcgccagtt gggtggtctg ggttttagat tgatcgaccg gtgtccaggt gagttgttgc
    15481 agcgcggcag atggaatggc tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg
    15541 gcaaggctga ttccaggtgc gcttgccagc aaccctgctg ataaacagag agcgatgaga
    15601 cttttattca ttttcattgt tttcacctca aaatctggag ctcagcggta gccaggcaat
    15661 agcgcgctaa acccgataat cagaggggct ttcgcccctt aaagctgttt ttatgccgga
    15721 tgcggcgtaa acgccttatc cggcctactt tcggcagcgg ttgtagttgt aggcctgata
    15781 agacgcgcaa gcgtcgcatc aggcgttggt tgccggatgc ggcgcaagca ccttatccgg
    15841 cccaggtttt gctattacca ccagatttcc atctgggcac cgaaggtcca ctcgtcgctg
    15901 tcaccacggc cgaagctgcc gccgttgaaa tcagcaggaa cggctttgcc gaagttagtg
    15961 ttggtattgg cgttaccggt gtagtcataa ccccatttct catcccactt ggcgtaggtt
    16021 gcgaagacac gaatagccgg gcgtgaccag atgctgtcgc cagcctgcca ttgttgtgca
    16081 agggtaattt tgtactgatt gttcttgtcg ccggtgcgct gggattcgac gttgtcgtag
    16141 ccgatttcca tcacggtgct catgattggc gtccacttgt acatcgggcg aataccgacg
    16201 gtccaccact tggtgccgtt gtcgttatcc cagttgatat cctggtacat acccacgtac
    16261 atcatgtccc agttgtcgcc catggagatc gcaccgtggt cgaggatacg cagcatgtga
    16321 ccgttgttgt tgatattgta ggcaaatttt tcgttatcaa acgcgacgcc agaaccctgc
    16381 gacagacctt taccctgcga ggtcatcgag tcagtagcgt actgaacaac aaacttgtta
    16441 aagcccttca ggacactctg agtatgttca gcagtgaata accaaccgtc tttcgatgcg
    16501 ccatcaacca gacgatagtt atcacgcagg ttggcacgac cgtagtcgac acccagttct
    16561 aatgtgccgc ccgggttgat ttccatctgc gctaaacgca cgtcgaaaac gtcgttcgcg
    16621 gtttcgttgg tatagtcata aatattgttg ctggcgaaag aggaagaacc accagcttca
    16681 gaggaacggg ttgctgccag agagagttta ccgaagccaa catcgatgtt ttccagaccg
    16741 gcaccaggac cagaaatatc ccagtagtag aagtcgatca tatgaacgtc atgacgttgg
    16801 tagaagcgct tacctgccca gatggtggaa cctggcagcc attcgatcag gtttttaccc
    16861 tgcacgttcg cttcacggaa ggccggatcg gtagcttccc agtcattctg ttgtgcgacg
    16921 gaataggcca cgttagtgtc gaaatagaag ctcttatcgc cctctttcca cacttcctga
    16981 cccaatttta attcagcata agtttcacat tcgttgccaa gacggtattt actttgagca
    17041 ccggtagtct ggaaacactg ttgttcaccg ccgctacctg tccagccaat accggaacgt
    17101 gcatagccgt ggaaatcaac agccattgcc tgagcagaca ttacgcccgc tgcgacggca
    17161 accgccagag gaagtttgcg cagagtaatc atcattctat ctcctgagtc attgcttttc
    17221 ttttttcaca tcacctgtga caggctttgt gtgttttgtg gggtgcttaa acgcccggct
    17281 ccttatgcag tcgacgacat gcagtgccat cctcacggaa cagatggcaa cgctctggcg
    17341 gcaggccgat agcgaatgtg gcaccttctt ctaccaacac cacgtcgttc tggcggtaca
    17401 ccaggttttg acgaatggaa gggatctgga tatggatttg ggtttcgttg ccgagttgct
    17461 cgacgacctg aacttcaccc tcaaggatga cgtcagcgat atcactcggc agtagatgtt
    17521 ccgggcgaat acccagcgac atattggctc caacctggac atcacggctt tcaaccggca
    17581 gccagacttg ctgacgattt ggcatcggca gctccacctg cacttgatcg attgcagtgg
    17641 cggtcacttt tactggcagg aagttcatct ttggcgaacc gataaatccg gcgacaaaac
    17701 ggtctgccgg atagtggtac agctccagcg gtttcccaac ctgcgccacg cgaccggcgt
    17761 ccagcaccac gattttgtcg gctagtgtca tcgcttcgac ctgatcgtgg gtgacgtaaa
    17821 tcattgtgcg gcccaggcgt ttatgcagac gggagatttc gatacgcatt tgcacacgca
    17881 gtgcagcatc gaggttggag agcggttcat cgagcaaaaa tacgcttggc tcggctacca
    17941 gcgtacggcc aatcgccaca cgctgacgct gaccaccgga gagcgctttc ggtttgcgat
    18001 ccagcaaatg cgccagttgc agcacttccg caacctggtt aactcgctgg ttaatcactt
    18061 cttttttcgc gccagccagt ttcaggccaa atgacatgtt ttctgctact gacaggtggg
    18121 gatagagcgc gtaagactga aacaccatac caacgccgcg ttctgcaggc ggagtgtcat
    18181 tcatccgttt ctcaccgatg aacaggtcgc cgctggtgat cgtctcaagc ccggcaatca
    18241 tgcgcagtaa agtcgattta ccgcagccag acggtccgac aaacaccacg aattcacctt
    18301 catggatatc gagattgata tctttcgata ccacgacctc gccccaggct ttcgttacat
    18361 tttgcagctg tacgctcgcc atgcccttct ccctttgtaa caacctgtca tcgacagcaa
    18421 cattcatgat gggctgacta tgcgtcatca ggagatggct taaatcctcc accccctggc
    18481 ttttttatgg gggaggaggc gggaggatga gaacgcagct tctgtgaact aaaccgaggt
    18541 catgtaagga atttcgtgat gttgcttgca aaaatcatgg cgattttatg tgcgcatctc
    18601 cacattaccg ccaattctgt aacagagatc acacaaagca acggtggggc gtaggggcaa
    18661 ggaggatgga aagaggttgc cgtataaaga aactagagtc cgtttaggtg ttttcacgag
    18721 cacttcacca acaaggacca tagattatga aaataaaaac aggtgcacgc atcctcgcat
    18781 tatccgcatt aacgacgatg atgttttccg cctcggctct cgccaaaatc gaagaaggta
    18841 aactggtaat ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga
    18901 aattcgagaa agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga
    18961 aattccctca ggttgcggca actggcgatg gccctgacat tatcttctgg gcgcacgacc
    19021 gctttggcgg ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc
    19081 aggacaagct gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt
    19141 acccgatcgc tgtcgaagcg ttatcactga tttataacaa agatctgcta ccgaacccgc
    19201 caaaaacctg ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg
    19261 cgctgatgtt caacctgcaa gaaccatact tcacctggcc gctaattgcg gctgacgggg
    19321 gttatgcgtt caagtatgaa aacggcaagt acgacattaa agacgtaggc gtggataacg
    19381 ctggcgcgaa agcgggtcta accttcctgg ttgatctgat taaaaacaaa cacatgaatg
    19441 cagacaccga ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacta
    19501 tcaacggccc gtgggcatgg tccaacatcg acaccagtaa agtgaattat ggtgtaacgg
    19561 tactgccgac cttcaaaggt caaccatcta aaccgttcgt tggcgtgctg agcgcaggta
    19621 ttaacgccgc cagtccgaac aaagagctgg cgaaagagtt cctcgaaaac tatctgctga
    19681 ctgacgaagg tctggaagcg gtcaataaag acaaaccgct gggtgcagta gcgctgaagt
    19741 cctatcagga tgaacttgcg aaagatccac gtattgccgc caccatggat aacgctcaga
    19801 aaggtgaaat tatgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg
    19861 cggtgatcaa cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga
    19921 ctcgtatcac caagtaatgc tgtgaaatgc cggatgcggc gtaaacgcct tatccggcct
    19981 acgaaattga aacgtatgta ggcctgataa gacgcgtcag cgtcgcatca ggcagttgtt
    20041 gtcggataag gcgtgaaagc cttatccgtc ctggaatgag gaagaacccc atggatgtca
    20101 ttaaaaagaa acattggtgg caaagcgacg cgctgaaatg gtcagtgtta ggtctgctcg
    20161 gcctgctggt gggttacctt gttgttttaa tgtacgcaca aggggaatac ctgttcgcca
    20221 ttaccacgct gatattgagt tcagcggggc tgtatatttt cgccaatcgt aaagcctacg
    20281 cctggcgcta tgtttacccg ggaatggctg gaatgggatt attcgtcctc ttccctctgg
    20341 tctgcaccat cgccattgcc ttcaccaact acagcagcac taaccagctg acttttgaac
    20401 gtgcacagga agtgttgtta gatcgctcct ggcaagcagg caaaacctat aactttggtc
    20461 tttacccggc gggcgatgag tggcaactgg cgctcagcga cggcgaaacc ggcaaaaatt
    20521 acctttccga cgcttttaaa tttggcggcg agcaaaaact gcaactgaaa gaaacgaccg
    20581 cccagcccga aggcgaacgc gcgaatctac gcgtgattac ccagaatcgt caggcgctga
    20641 gtgacattac cgccattctg ccggatggca acaaagtgat gatgagctcc ctgcgccagt
    20701 tttctggcac gcagccgctc tacacactcg acggtgacgg cacgttgacg aataatcaga
    20761 gcggcgtgaa atatcgtccg aataaccaaa ttggctttta ccagtccatt accgccgacg
    20821 gcaactgggg cgatgaaaag ctaagccccg gttacaccgt gaccactggc tggaaaaact
    20881 ttacccgcgt cttcaccgac gaaggcattc agaaaccgtt cctcgccatt ttcgtctgga
    20941 ccgtggtgtt ctcgctgatc actgtctttt taacggtggc ggtcggcatg gttctggcgt
    21001 gtctggtgca gtgggaagcg ttgcgcggca aagcggtcta tcgcgtcctg ctgattctgc
    21061 cctacgcggt gccatcgttc atttcaatct tgattttcaa agggttgttt aaccagagct
    21121 tcggtgagat caacatgatg ttgagcgcgc tgtttggcgt gaagcccgcc tggttcagcg
    21181 atccgaccac cgcccgaacg atgctgatta tcgtcaatac ctggctgggt tatccgtaca
    21241 tgatgatcct ctgcatgggc ttgctgaaag cgattccgga cgatttgtat gaagcctcag
    21301 caatggatgg cgcaggtccg ttccagaact tctttaagat tacgctgccg ctgctgatta
    21361 aaccgctgac gccgctgatg atcgccagct tcgcctttaa ctttaacaac ttcgtgctga
    21421 ttcaactgtt aaccaacggc ggcccggatc gtcttggcac gaccacgcca gccggttata
    21481 ccgacctgct tgttaactac acctaccgca tcgcttttga aggcggcggg ggtcaggact
    21541 tcggtctggc ggcagcaatt gccacgctga tcttcctgct ggtgggtgcg ctggcgatag
    21601 tgaacctgaa agccacgcga atgaagtttg attaagggag ataacaaaaa tggcaatggt
    21661 ccaaccgaaa tcgcaaaaaa cacgtttgtt tattactcac ctgctactgc tactttttat
    21721 cgcggcgatt atgttcccgc tactgatggt cgtcgctatc tcactgcgcc aggggaactt
    21781 tgcgaccggc agcctgatcc cggagcaaat ctcctgggat cactggaaac tggcattagg
    21841 ttttagcgtg gaacaggctg atggtcgcgt tacaccgccg ccgtttccgg tactgctatg
    21901 gctgtggaac tcggtaaagg tcgccgggat ttccgcgatt ggcattgtgg cgctctccac
    21961 cacctgcgcc tacgctttcg cccgtatgcg ctttccaggc aaagcgacgc tgctgaaagg
    22021 aatgctgatt ttccagatgt tcccggcagt actttcactg gtcgcgttgt atgcgttgtt
    22081 tgatcgtctg ggtgagtaca ttccattcat tggcctgaat actcacggcg gcgtaatttt
    22141 cgcgtatctg ggcgggattg cgctgcatgt ctggaccatc aaaggttatt tcgaaaccat
    22201 cgacagttcg ctggaagaag cggcggcgct ggatggtgcg acaccgtggc aggccttccg
    22261 ccttgtcctg ttgccgctgt cagtaccgat tctggcggtt gtgttcatcc tgtcgtttat
    22321 cgctgccatt actgaagttc cggtcgcgtc gctattactg cgtgacgtaa acagctacac
    22381 cctggctgtg gggatgcagc aatacctcaa cccgcaaaac tacctgtggg gtgactttgc
    22441 cgccgctgcc gtgatgtctg cattaccgat caccatcgtc ttcttgctgg ctcaacgctg
    22501 gctggtcaac ggcctgacgg caggtggtgt gaaaggttaa agatgttgtt ctgccaatgt
    22561 tatgccgctg caccctcaac ttacgttatc ccaacttgtg actgttattc ggcgctccac
    22621 ggagcgcctt tttttactcc cgcttcagcc gtttcgaatt acacaaccac agggtgatca
    22681 ccagtaacag gatcgctgcc gagtagatca acacatccag tggcgattta tgatcgacga
    22741 tgatcaagcg cacaatggcg gtgatcccaa tatagacaaa gtaacgtagc gggaagtgaa
    22801 aaccggactg aaagtacttc acaatcagcg cgataaattc gaaatagaga aagtaaacca
    22861 ccagcccttc taccagctca tatttactgg tttgttctgg cgcgaacagc acatcagcca
    22921 gatgcaccgt ttctttgccg aggaagacaa ccaaaatcag gccgaggcac aacaggccaa
    22981 gattgagtac ggtctgcaaa atggtggata taaactccac gcgcggacga gagagtgacg
    23041 tcatagcagt acctccttca cagtggcgag cttcctgtat aacgcaaaaa tgtgacggag
    23101 atctatattt tttgtgtatg ttttgttcag ctcaattaag tgggcatctc ccatcaatgt
    23161 ctcatctggc aaaacacaca gccaaaaaaa tcacccattc aggtgattcc cagccaccag
    23221 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    23281 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    23341 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagtcc
    23401 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    23461 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    23521 ctcgacaggc cattaagaat gagggtgcca gtgagtgcga aaactggcac cttttccatg
    23581 acttagcgga aattgacgct acggtcgctg gtcatgtcat acaactggaa cttacgccca
    23641 agttgctgac caccgtcacg cgtcagcggc gtccagccaa ttgccgcacg gctacgtgtc
    23701 gggccagacg agaagagatc cagcggtaca gagacataca cgcctttggt aaagtcccct
    23761 tcgccgtact cctctttcga aacgttggtg atcgtggcat agccacccac caccacgccg
    23821 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    23881 acgctcgctt tcactaacac atcctgagca aaagatggcg tccagtatgc ggtcagatgt
    23941 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    24001 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg gcgatacaac
    24061 acttctgccc ccgcaccgcc aaacatggtt tcgagataac ctccgtagac ctgaccgtag
    24121 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata gacatcgttc
    24181 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg cggcggattg
    24241 gtgtagttaa acttgtcgta gttgttggca aggttagcaa acaggctgcc ggtggtcagc
    24301 agatggtccg tcagccacaa atccgccgtt cccatcacgc ccagctgata catgtaaaag
    24361 ttttccgggc caccgaccga ctggttcaac accggatcga tatggaaatc aaagcgtgat
    24421 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    24481 gccagcttcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    24541 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    24601 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcacgcgaa
    24661 tcacgatatt tcacctgctc gccggtgaca tacagcgtat cgcctttcgt ctggatctgc
    24721 ggatcggcca gtccggcgtt gtatttcaac agcgttaact gattcgccac caccgaatgc
    24781 tgcaaaatgg catcctgtgg ttgcggttga tattgcgggc gggcgttatc gttgtaggac
    24841 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaagt gttgccacgt
    24901 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    24961 ttactttttt gctccagctt accggcaaaa tcctgctgat aattattgcc ttcatactcc
    25021 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    25081 ggaccgtgga acatctggct gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    25141 tagcaatatt tgtcactggc cgaacagagc ggatttttca cattaccgct ggtgcccaga
    25201 tatccccagc ccaggccgag cgtaaaatcg aatggccccc aggctttgct ggcgacaaga
    25261 tattccgcat cgaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    25321 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    25381 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtagcgc
    25441 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    25501 ttatcgcgat agttcagact cagctcccct tcccgcgcca tgcgcgcggt gggcgtttgt
    25561 aataatccta cgccaccgaa atccgattgc gacggaccaa tgggcgcagg atatgtttcg
    25621 ccgtagcaag ctgtgctaat acccagcgcc agtaagctaa gcagatgtct ttttttcatt
    25681 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggtgtct
    25741 cactccagac ggagtccgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    25801 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaaccaccc
    25861 acgcgtagct gcgatccgca ccgctaagca ggttctgccc ggagagatag ctcgccacgt
    25921 cgcgacctgg agtgaatggc tgcttgccag gacggctgat aagcccgaac aacgtgacag
    25981 tggacggtgg tggtccgacc cacagtgtgt agttgccctg caacggtggg ttaccacgtt
    26041 cggcaacgcg cacgatatcg ggatcaagat tgattttttg cctgcccgtc actttcaacg
    26101 cctgaatttg ctggcgtaag gcgttaatcg cagcggcatc gtcagcgctg gaatctgcgg
    26161 ccagttctgc cagccgtgcc agtagcgcct gctgctgccg caatgctgcc gccgttgcca
    26221 gctcttcgct gatcactgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    26281 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    26341 ccttgacggt tcctgccgcg aagaccgatg acgcccccac actcaaaatc aatgcgacaa
    26401 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    26461 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    26521 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaaccg gggtttcatc
    26581 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    26641 gcgtacctgc tggtactcga tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    26701 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    26761 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgacg agagtggcct gatcctgagt
    26821 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaataact gcggcccgcc
    26881 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    26941 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    27001 tgaacaggcc tgtaatagca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    27061 ccagaggggc agaataacca caccggagtg tggttatggt taatcccctt catacttcaa
    27121 gctgcagatg cggtgaccgc actcgttcac cccggtcaca tagttatcta tgctcctggg
    27181 gactcactcg cttgtcgcct tcctgcaact cgaattattt tggggataca tactttatta
    27241 ctgggtactg gtggtcgtgg ttgttgtggt cccggtatta gaaccatcac cgccaccgct
    27301 tgccgcgagc gcgacaccca ccgccgagct tacggtgctg acgctggtcg cggaggaact
    27361 ccccgccgaa accgacgttg ctgccgaccc tgccgcttcg cccacctgta caggtgcagc
    27421 ccacgcagaa gtcgccgcaa gcgcagatat ggcaaaaatg ccatacagaa cttttttcat
    27481 aacaatttcc cttcattgaa tgaatggaga tttacccaaa aacatttcgg gcgggatcga
    27541 gtatacacaa ctaaagcatg cgaattacgg cgcggggaat tagtgatggg gttttaggat
    27601 aattggaatg aatatcaaat ataagcttca gcttatatat aaatagcact aaaaatcata
    27661 agcttaaaat aaaaataaaa gtaattaatc tgcgtatttt ccgaaatcaa tcacgattaa
    27721 aaatctattt taggattaac ctcacggtat gatttccgtt aaatcacaga caaggggatt
    27781 tatctgataa aaaatgccga cataacgtcg gcattgtttt taaggctatt taatcaatcg
    27841 tactatgtat taattgccga atacgatgta catatcggca tcgacctgta ggcctgataa
    27901 gacgcgaccg cgtcgcatca ggcatcggtt gccggatgcg gcgtgaacgc cttatccggc
    27961 ctacatatcg acaatgatta accgcgccac gctttatagc ggttaatcag accattggtc
    28021 gagctatcgt ggctgctgat ttctttatca tctttcagct ctggcagaat acggttcgcc
    28081 agctgtttac ccagctccac gccccactgg tcgaaggtga agatgttcag aatcacgccc
    28141 tgagtaaaga ttttgtgctc atacagcgca atcaatgcac ccaggctgaa cggagtgatt
    28201 tcacgcagca ggatggagtt ggtcgggcgg ttaccttcga acactttgaa cggcaccacg
    28261 tagtcaagcg ttgccggatc tttaccctga tcacgatatt cctgctcaac cacttcgcgg
    28321 gatttaccaa acgccagcgc ttcggtctga gcaaagaagt tagacagcag tttctggtga
    28381 tgatcagaga gcgggttatg ggtgatagcc ggagcgatga aatcgcacgg taccattttg
    28441 gttccctggt ggatcagctg atagaacgcg tgctgaccgt tcgtgccagg ttcaccccag
    28501 ataatcgggc cagtctggta atccacaacc ttaccgttac ggtcaacata cttaccgttg
    28561 gattccatat tgccctgctg gaagtacgcc gcgaaacggt gcatatactg gtcatacggc
    28621 agaatcgctt cagtttccgc gccaaagaaa ttgttgtacc agatgccaat cagcgccagc
    28681 aagacaggca ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat cgcgtgtgcg
    28741 ccagaaagca gttcaacaaa attatcaaag ccgatggaga gaacaatcga caggccaatt
    28801 gctgaccaca aagagtaacg gccgccaacc cagtcccaga actcgaacat gttggcagta
    28861 tcaataccaa actcgccaac ggctttggca ttggtggaaa gcgccgcaaa gtgtttcgca
    28921 acgtgttttt catcaccagc cgctttcagg aaccagtcac gcgcgctgtg ggcgttggtc
    28981 atggtttcct gagtggtgaa ggttttagat gctaccagga acagcgtggt ttccgggttt
    29041 acttttttca gcacttccgc gatgtgagtc ccatcgacgt tagaaacaaa gtgcatgttc
    29101 aggtggtttt tgtatggacg cagtgcttca gtcaccatgt acgggccgag gtcagaaccg
    29161 ccgatcccga tgttcactac gtcagtgatt gctttgccgg tatagccttt ccactcaccg
    29221 gaaataatcg cttccgagaa ggttttcatc ttctccagca ccgcgttgac ttctggcatt
    29281 acgtctttgc catcaaccag aatcggggta ttgctacggt tacgcagcgc tacgtgcagc
    29341 acggcgcggt tttcagtgcg gttgatcttc tcgccagaga acatcgactt aatcgcgccc
    29401 gccagatcgc actctttcgc cagatcttgt aatttcgcca gcgtctcttc agtgatgcgg
    29461 tttttggagt aatccaccag catctgatcg tcgaatgttg cggagaactt agaaaaacga
    29521 tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc gaagtgtttc
    29581 tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt ttttcattag caatactctt
    29641 ctgattttga gaattgtgac tttggaagat tgtagcgcca gtcacagaaa aatgtgatgg
    29701 ttttagtgcc gttagcgtaa tgttgagtgt aaacccttag cgcagcgaag catttattag
    29761 ctgaactact gaccgccagg agtggatgaa aaatccgcat gaccccatcg ttgacaacgg
    29821 ccccgctcac cctttattta taaatgtact acctgcgcta gcgcaggcca gaagaggcgc
    29881 gttgcccaag taacggtgtt ggaggagcca gtcctgtgat aacacctgag ggggtgcatc
    29941 gccgaggtga ttgaacggct ggccacgttc atcatcggct acaggggctg aatcccctgg
    30001 gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac ttctgggtga
    30061 aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc gctcttccct tgtgccaagg
    30121 ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa attgttgtct ccaaatttgg
    30181 cggtaccagc gtagctgatt ttgacgccat gaaccgcagc gctgatattg tgctttctga
    30241 tgccaacgtg cgtttagttg tcctctcggc ttctgctggt atcactaatc tgctggtcgc
    30301 tttagctgaa ggactggaac ctggcgagcg attcgaaaaa ctcgacgcta ttcgcaacat
    30361 ccagtttgcc attctggaac gtctgcgtta cccgaacgtt atccgtgaag agattgaacg
    30421 tctgctggag aacattactg ttctggcaga agcggcggcg ctggcaacgt ctccggcgct
    30481 gacagatgaa ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg tcgagatcct
    30541 gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt aaagtgatgc gtaccaacga
    30601 ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg gaactggccg cgctgcagct
    30661 gctcccacgc ctcaatgaag gcttagtgat cacccaggga tttatcggta gcgaaaataa
    30721 aggtcgtaca acgacgcttg gccgtggagg cagcgattat acggcagcct tgctggcgga
    30781 ggctttacac gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct acaccaccga
    30841 tccacgcgtg gtttccgcag caaaacgcat tgatgaaatc gcgtttgccg aagcggcaga
    30901 gatggcaact tttggtgcaa aagtactgca tccggcaacg ttgctacccg cagtacgcag
    30961 cgatatccca gtctttgtcg gctccagcaa agacccacgc gcaggtggta cgctggtgtg
    31021 caataaaact gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca atcagactct
    31081 gctcactttg cacagcctga atatgctgca ttctcgcggt ttcctcgcgg aagttttcgg
    31141 catcctcgcg cggcataata tttcggtaga cttaatcacc acgtcagaag tgagtgtggc
    31201 attaaccctt gataccaccg gttcaacctc cactggcgat acgttgctga cgcaatccct
    31261 gctgatggag ctttccgcac tgtgccgggt ggaagtggaa gagggtctgg cactggtcgc
    31321 gttgattggc aatgacctgt caaaagcctg cggcgttggc aaagaggtat tcggcgtact
    31381 ggaaccgttc aacattcgca tgatttgtta cggcgcatcc agccataacc tgtgcttcct
    31441 ggtgcccggc gaagatgccg agcaggtagt gcagaagctg cattttaatt tgtttgagta
    31501 aacacagtat ggcccggaag acattatttc gggccgtatt gtttttcttg tcattatgcc
    31561 cataaataaa cgagcctgta ctctgttaac cagcgtcttt atcggagaat aattgccttt
    31621 aattttttat ctctttctct aactaattag agaaagagat aaatagttaa gagaaggcaa
    31681 aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc tctggctcac
    31741 tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg aacgcgagca
    31801 gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg tactgaccaa
    31861 ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg agcgtgaggc
    31921 tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg atcctgaggc
    31981 gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctgattaa cgccccttct
    32041 ttttgcgccc cggcgaggta aaacgcttac cgttggacgc cgggcgaccg cctttttccg
    32101 ccgttttttc catcttaacg actggacgtt tgatgcctac cgttttcggt ttggccttcg
    32161 ctttaggttt tgcctcagaa gaggaatttt cgatgagctt aaagaggtcg attaactcat
    32221 cgtcggttaa atcgcgccac tcccccagcg gaatgccgct taaactgacg ttcataatgc
    32281 gcgtacgttc cagctttttc acttcgtaac caaagtgctc gcacatgcga cggatctgac
    32341 ggttcagccc ctgcaccagg gtaatgcgga agacaaatgg cgcttctttt ttgactttgc
    32401 actttttagt cactgtcccg aggattggca cccccgcccc cataccacga ataaattcat
    32461 cggtaatcgg tttatcgacc gtcaccagat actctttctc atgatcgtta cccgcacgga
    32521 ggatcttatt caccagatcg ccgtgattgg tgaggaaaat cagcccctgg gagtctttat
    32581 ccaggcggcc aatcgggaat acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc
    32641 gctcgccatc ttcggtggtg ctgacaatac cgacgggctt gttcagggcg ataagtacca
    32701 aatcttcggc ttcccgaggt tcaatcaact gaccatttac tttcacaacg tcgccgggtt
    32761 tcacctgatc gccaatggtg gctcgcttgc cattaaggaa cacattgcct tgctcgatat
    32821 agcgatccgc ttcgcggcgt gagcaaattc cgctttcgct gatgtattta tttaaacgga
    32881 ctgatgagtc gggcagcata aattctcctg taaaacgcgg aatataccgt acctcttagc
    32941 ggggaaaaac agggttatgc ggcaacgtcg aaattaaatg tgatctgcca cgccttttat
    33001 cacaaaagtg tctccggaag tacgatgcaa atttgcctgg caaaacaagg ataatcaaca
    33061 tattacgaat cgctgcacac ctgtgctgat gactactacg cagagaaaca aatgtgcaag
    33121 agaaagcgct atttctcccc atgcaaagag tgaaaaatcg ctttgaaagg ccctcatgat
    33181 ggggttatat cagatcaagc ggtgagaaag cgtatttgca caaatgtgca gaaccaggag
    33241 gcggcaatgg aaaacagtga cgatatccgt ttgattgtga agattgccca actctattac
    33301 gaacaggata tgacgcaggc gcaaatcgcg cgcgaactgg gtatttaccg caccaccatc
    33361 agccgcttgc ttaaacgagg ccgcgatcag ggaattgtca ccatcgccat caactatgac
    33421 tacaacgaaa atctctggct ggagcagcaa ctgaagcaaa agtttggcct gaaagacgtt
    33481 gtggtggtgt caggaaatga tgaggatgaa gagactcaac tggcgatgat ggggttacac
    33541 ggcgcgcaac tgctggatcg cttgctggaa cctggcgata ttgtcggttt ttcctggggc
    33601 cgcgcggtga acgcactggt tgaaaacttg ccgcaggcgg ggcaatcgcg gcagttaatc
    33661 tgcgtaccaa ttattggcgg accgtccggt aaactcgaaa gccgctatca tgtaaacaca
    33721 ttaacctaca gcgcggcagc gaagttgaaa ggggaatcgc atctcgcgga ttttccggct
    33781 ctgctggata acccattaat tcgtaatggg atcatgcagt ctcagcactt taaaaccatc
    33841 tctgcctact gggataatct ggatgtcgcc ctggtgggaa ttggctcacc ggccattcgc
    33901 gacggcgcta actggcatgc gttttatggt ggtgaagaga gtgacgacct gaatgcccgc
    33961 caggttgctg gcgatatttg ctcgcgcttt tttgatattc acggcgcaat ggttgaaaca
    34021 aatatgagcg aaaaaacact ctctatcgaa atgaataaat taaagcaggc gcggtattcc
    34081 attggcattg ccatgagtga agaaaaatac agcggaatta ttggtgcact gcgtggaaaa
    34141 tatattaatt gtctggtaac gaatagcagc acagctgaac tattactgaa ataaaaatct
    34201 tcccgatgtt taccgtaata aaaattacgg cgggtctcta ttatctaaaa tcaggagtct
    34261 gttatgcaaa cgtggttaaa tttgcaggat aaaatcatta ttgtcaccgg tggcgcatcc
    34321 ggcattggtc tggcgattgt ggatgaatta ttagcacaag gcgcgaatgt acagatggtc
    34381 gatattcacg gtggcgatgg acaatatgaa ggtcataaag gctatcagtt ttggccgacc
    34441 gatatttcca gcgccaaaga ggtaaatcat acggtagcgg aaattatcca gcgttttggt
    34501 cgcatcgacg gtctggtcaa taacgccggg gtcaatttcc cgcgtctgct ggtcgatgag
    34561 aaagcgcctg ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat ggtcaatatc
    34621 aaccagaaag gcgtttttct gatgtcgcag gcggtggcgc gacagatggt caaacaacat
    34681 gatggcgtga ttgtgaatgt ttcctcagaa agtgggctgg aaggctcaga aggccaaagc
    34741 tgttacgccg ctaccaaagc cgcgcttaat agcttcacgc gatcctggtc gaaagaattg
    34801 ggtaaacacg gtatccgtgt agtcggtatc gcgccgggga ttctggaaaa aaccaggcta
    34861 cgcacgccgg aatatgaaga ggcgctggcg tggacgcgca atatcaccgt cgagcagctg
    34921 cgtgaaggct ataccaaaaa cgccattcct attgggcgcg ccggaagatt agcagaagtc
    34981 actgattttg tttgttatct gctgtctcaa cgcgccagct atatcaccgg agtaaccact
    35041 aacattgcgg gcggcaaaac gcgcggctaa ggaggcttta tggtcaacgc cattttttgt
    35101 gcccacggca aattagcttg cgccatgctg gaatcggttc agatggttta cggcaacgcc
    35161 aatgttgaag ccgttgaatt tgtgcccggt gaaaacgccg gagacatcgt agcaaagctg
    35221 gaaaagttag taagcattca caatcaagat gagtggctga ttgccgtgga tttacagtgc
    35281 ggcagcccgt ggaatgcggc agcgatgctt gcgatgcgca atcctcgtct gcgggtaatc
    35341 agcggccttt ctctgccgct ggcgctcgaa ctggttgata accagcacag catgaatgtt
    35401 gatgaactgt gtgaacacct gacgcaaatc gcaaagcaaa cctgcgttgt ctggaaacaa
    35461 ctggcaacgg cagaggagga tttctgatga acattacgct cgcccgcatt gatgaccgtc
    35521 tgatccacgg ccaggtcact accgtctggt cgaaagtggc taatgcccag cacattatta
    35581 tctgcaatga cgaagtttat aacgatgaag tgcgccggac attattgcgt caggctgcgc
    35641 caccgggaat gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt tatcacaacc
    35701 cgcaatatca ggacgaaacc gttttttatc ttttcactcg cccacaggat gccttagcga
    35761 tggttcgcca gggggtaaaa atagacaccc tgaatattgg cggcatggcc tggcggcccg
    35821 gtaaaaaaca gttaaccaaa gcggtttcat tagatgatga tgatattaac gcatttcatg
    35881 agttaaataa tcttggcgtc atactggatt tacgcgtcgt agcgtcagat ccctcaatca
    35941 atattatcga caaaataaac gaacagttaa tcgcaaatta aataatagcg cctgtgtaca
    36001 tatgctttaa caggcaggga tgacgacact taaaaggtga caaatcatgg aaataagtac
    36061 cctacaaatc attgctatat ttcttttttc ctgtattgcc ggaatgggca gcgtgctgga
    36121 tgaatttcag actcatcgtc cgttaattgc ctgtacggtg attggtttaa ttctcggtga
    36181 tttaaaaacc ggaattatgc tcggtggtac gctggaattg atagctctcg gctggatgaa
    36241 cgtcggcgcg gcgcaatctc cggattctgc actcgccagc ataatctccg ccattctggt
    36301 tatcgttggt cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg tggctgcggc
    36361 aggccaggtg ctgacggtgt ttgcccgtac catcaccgta gtgttccagc acgcggcgga
    36421 taaagcagcg gaagaagcac ggtttcgcac tctcgatatt ctgcatgtct ccgcgcttgg
    36481 cgtgcaggca ctgcgcgttg ctattccggc actgattgtc tcgctgttcg tcagcgctga
    36541 tatggtgagc aatatgctga gcgccattcc cgaatttgtg acccgtggac tgcagattgc
    36601 tggcggtttt atcgtggtgg tcggttacgc catggtgctt cgcatgatgg gcgtgaaata
    36661 tttgatgcct ttctttttcc tcggtttcct cgcaggtggc tacctcgatc tcagtctgct
    36721 ggcgttcggt ggcgtcggcg tgatcatggc cctgctctac atccagctaa atccacagtg
    36781 gcgtaaagct gaacctcaac tccaggccac cgcctccacc gcccttgacc aacttgacga
    36841 ttaacggagc catcatggaa cagagaaaaa ttacacgcag cgatctggtg agcatgtttc
    36901 tgcgctccaa cctgcaacag gcgtccttta actttgaacg tattcacggg ctgggttttt
    36961 gctacgacat gatccccgcc atcaagcgac tttacccatt aaaagaggat caggttgcgg
    37021 cgctcaggcg acacctggtg ttcttcaata ccacgccagc cgtatgtggc ccggtcatcg
    37081 gcgtcactgc cgccatggaa gaggcgcggg ccaacggcgc ggaaattgat gacggtacca
    37141 ttaacggcat caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct
    37201 ggggaacgct gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca
    37261 ttctcggccc gctgctgttt ttctttattt tcaatgcggt gcgtctggcg atgaagtggt
    37321 atggcctaca gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg
    37381 tgctgcaaaa actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg
    37441 tgaccaaatg gacgtcaatc aacgtaccgt tgctggtttc acaaacgcat gccgccgatg
    37501 gctccaccgt caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg
    37561 cgctcggtct gacgctgctg atggttcgtc tgctcaacaa aaaaattaac ccggtatggc
    37621 tgattttcgc cctgtttggt ttagggatta tcggtaatgc gctgggcttc ctgtcctgat
    37681 tcttcgcccc ggcacgactg ccggggtcat cgctcaacat gaggtggttt atgaaaacaa
    37741 cagctctgcg tctttatggc aaacgtgacc tgcgtctgga aacctttgac cttcctgaaa
    37801 tgcaggagga tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga
    37861 aagaggccaa tctgggtgaa aaccataaaa aagtacccga cgatgtggcg accaacccca
    37921 tcatcatcgg ccacgagttt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca
    37981 aattccagcc gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg
    38041 attgccccgg ctactccttc ccgtgggtag gcggcgaagc gacgcatgtg gttattccca
    38101 acgaggtcat ggaacaagat tgcctgctgg catacgacgg cgaaacctat tttgaaggct
    38161 cgctggttga accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg
    38221 aaggtagtta taaccacacg atggggattc gcccgcaagg gcgcacgctg atcctcggcg
    38281 gcaccggccc gatggggctg ctggcgattg attatgcgtt gcatggaccg gttaacccgt
    38341 cgctgctcgt cattaccgat accgacaacg acaaattgag ttatgcacgc aagcactatc
    38401 catcagaacc gcaaacactg attcattatc tcaatgccgc cgatgcagca tttgatacgt
    38461 taatggcact gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag
    38521 gactggtgac tctcgcctct tccttgctgg cgacagatgg ttgtctgaat ttcttcgccg
    38581 gaccgcagga taagcacttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta
    38641 cccactacgt gggcacgtca ggcggcaata ccgacgacat gcgcgcagcg gtcaaattga
    38701 ttgaagagaa aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg
    38761 cgggcgaaac cacgcttgaa ttgcctgcca tcggcggcgg caaaaagctg gtgtataccg
    38821 ggaaatacct gccgcttatg tcactcacgc agattcagga tcaagcactg gcggcaattc
    38881 tggcgcgtca tcaggggatc tggtccggtg aggcggagca atacctgctc gcccatgcag
    38941 aggcaatttc ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgccgc
    39001 ggcaacttcg cgacctgatg tgcggctgat tgctgaagcg gcacaatgtg cacgagaagg
    39061 ataaaaaaac atcagcgcct taaggggcgc tcaaattggt ggcaaagagc acattgttta
    39121 tgccggatgc ggaatgaagc cttattcggc ctacataatc ttgaaaattc aatatattgc
    39181 agttatctgg taggccaggt aagcgtagcg catcaggcaa tcctgtgttt gtcatcagtc
    39241 tcagcgcctg tgcaatatgt gacatcgtga gccatgtcag taagtggtta aattcgcagt
    39301 ggtagcattc cttccgtgtt ttcttactaa ccggagaagt aatggaactg cttttattga
    39361 gtaactcgac gctgccgggt aaagcctggc tggaacacgc gctgccgctg attgctgaac
    39421 agttgcaggg tcgccgttct gcggtattta ttcctttcgc gggcgtaacg cagacctggg
    39481 atgattacac agcgaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta
    39541 ttcatagcgt tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg
    39601 gcggtaatac tttccagttg ctgaaacagt gccgcgagcg cggactgctg gcaccaatta
    39661 ctgacgtggt taaacgtggc gcgctgtata ttggctggag cgctggcgct aaccttgctt
    39721 gcccaaccat tcgtaccacc aacgatatgc cgattgtcga tccacaaggt ttcgatgcgc
    39781 tgaatctgtt cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata
    39841 aaggtgaaac ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactcacaa
    39901 ttattggtct accggaaggt aactggatca cggtgagtaa aggtcacgct acgctgggtg
    39961 gcccgaacac cacttatgtg tttaaggctg gtgaagaagc ggttccgctg gaagcgggtc
    40021 accgttttta agtgaattgc cggatgcatg atccggcatc ttgtacatag tgcctgatgc
    40081 gacgctttac acgtcttatc aggccttcag ggtcacggta ttaatactcg tcccgtcctt
    40141 cgtcttcatc cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcagac
    40201 gctgcatatc tcccagtaag cctaaatgta gcgaactggt ttcaatactc tgcacgtttt
    40261 gctgatgcag gcgatcgacg tgagcatggg aataacggcg attaagaatg cgaaagcgat
    40321 gcttgctgcg gcgcaaacga cgggcgctgg taacatcgcc agagaagaaa accgacattg
    40381 ccagttttaa attactgagc aattgctcat agagcgcatc cagttccttc aagccatcaa
    40441 gcgaaaatgc ccgccgtgct gccagcgatt tatcggcaat ttcactcccc atgcgctcga
    40501 cgatatcgga ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg
    40561 actcttcttc tgccagttcc tcttttggca tccgcgccag atacagctta atggcggtat
    40621 agagaacgtt gatgtcatca gccagcttac gcagctcttt ctcctgccgt ggctcgccgt
    40681 gcatcacttt attcaaccct tccatcatct gttccatggc gtcgccaatg cgcagggttt
    40741 cgcgggcagc gttcgccaga gcaagcgtgg gcgtatccag cgcgctgaca tccagatgtt
    40801 taggccgtag ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg
    40861 ccatcgggtc aacaaatggc agcatgacca ggcagcgtac aaggttgtag aagacgtgga
    40921 aatagatcac caattccgct tttggcaatg gcaacttccc cattgtctct gccagcaaat
    40981 gaacaaacgg caggataatc aggctaccca ccagcttaaa cagcaggcta cccagcgcga
    41041 cacggcgggc tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga
    41101 ggtttgcacc aatcaccaga caaagcgcca ctgggaaaga gataatgcct gcggcggtca
    41161 gagtcgcggt cagcagtacc gcggcaaggc tggagtagct gataatggcg aacatcgcac
    41221 caatcagcgc atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt
    41281 ttgcctgggt gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcaacc
    41341 caagaccaat accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa
    41401 aaatcacgcc gataaaaata agtaatggtg acagccagga taagtcgaag gtgagaatac
    41461 gcgccattag cgccgtcccg acatctgcac ccagcacaat caccagagcc ggtgcgaggg
    41521 ctaccagatc ctgagcgacg aacgaggtca ccagcatagt ggtggcatta ctgctctgca
    41581 ccagtgcggt aacgcctatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc
    41641 taaggacagt acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag
    41701 tcccccagac cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag
    41761 gttctcctta tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc
    41821 tgactggcat ctttactgtg ccttttgacc ttcgtttttc cggctaagat tattccagcc
    41881 tgtttccagt ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt
    41941 ttgttgtaag gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg
    42001 ccagccagcg ctcaacatct gagacgctca tacctttacg gcgggcatag tcttcaacct
    42061 gatcgcgctg gatttgcgcc accgcgtaat acttgctgtc cgggtggctg aagtaccaac
    42121 ccgaaaccga tgcaccaggc cacatggcga aagattctgt gagtttcatg ccagtgtgtt
    42181 tttccacttc cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat
    42241 aacccggtgc cggacggatg ccctggtagt tttcgcggat cagctcttcg ttgctgaggt
    42301 tctcgttcgg cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa
    42361 aggcttcggc taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct
    42421 gcgcttcaaa ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc
    42481 cgatgtaatc tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag
    42541 cgaagccggt tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtac
    42601 gcgtttcgtc acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca
    42661 ccacgccacg cggattcagc gttttttcgg cgcttaattt atccagcatg tcgttggcgt
    42721 ctttaaacag ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact
    42781 tcccggacag cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt
    42841 cgatgctggc ttcgacttcc tgcacaccga gacggtgcgc caccggcggc gtgtaagcct
    42901 gccagtcaaa agcgaagtcg ttatcacgcg ccgcttccag cgtgaccggt ggtgtgcgtg
    42961 gtttctttcg tccgtgctga atacgcacgg tttcgtactc cttgcgggta cgagcaacga
    43021 aatcatcgcg ctgagtgtcg gaaagcaacg ccgccaccac accaacggtg cgtgaagcgt
    43081 tctgcacata caccgtcggg ccgctgtagt tctgctcaat tttcaccgcc gtgtgcgctt
    43141 ttgaggtggt cgcgccgcca atcaatagcg gaatggtgaa gccctgacgc tccatctctt
    43201 tcgccacgtt aaccatctcg tccagcgacg gcgtgatcag ccccgaaagg ccaatcaaat
    43261 cagcattcac ttctttagcg gtacggagaa ttttttccgc aggcaccatc acgccgagat
    43321 cgacaatttc gtagttgtta cattgcagca ccacgccaac gatattttta ccgatgtcgt
    43381 ggacatcgcc cttcactgtg gcgatcacca tcttgccgtt ggttttaccc tgctctttgc
    43441 tggcttcaat gaacggctcg agatacgcca ccgcctgttt catgacgcgc gccgatttca
    43501 ccacctgcgg caggaacatt ttgccctcgc caaacaggtc gccgaccacg ttcatgccgt
    43561 ccatcaacgg gccttcaatc acttcaatcg gacgcgtagc ctgctggcgg gcttcttcgg
    43621 tatcctgctc gataaactcg gtaatgcctt tgaccagcga gtattccaga cgtttattca
    43681 cttcccacga gcgccactct gcctgctggg tgttagcggt gtcgtcggtt ttgctgccgc
    43741 gatatttctc ggcaagctcg agtaaacgct cggtgccatc gtcgcgacgg ttgaggatca
    43801 catcctccac cgcgtcgcgt agctcggtag gcaggtcgtc gtaaatcgcc agttgcccgg
    43861 cgttgacgat ccccatatcc atgccattgc gaatagcgta gtagaggaac accgcgtgaa
    43921 tggcttcgcg caccggatcg ttgccacgga atgagaaaga aacgttagaa acgccgccgg
    43981 aaatcagtgc gtgcggcagt tcacgtttga tgtcttcaca cgcgccgata aagtcctgcg
    44041 cgtaattgtt gtgctcttca atgccagttg cgaccgcgaa gatgttcggg tcgaagatga
    44101 tatcttcagc cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct
    44161 cgattttccg tgcgcgggta tcggcctggc cctgttcgtc aaaagccatt accacgactg
    44221 ccgcaccgta gcgacgcaac agtttcgcgt gatggataaa ggcgtcgacg ccctctttca
    44281 tcgagataga gttaacaatg cctttgccct ggatacactt cagacctttt tcaataacgt
    44341 cccattttga ggagtcgatc attatcggca cgcgagcgat atccggttca ccggcaatca
    44401 gattgagaaa acgcaccatc gccgcttcgg cgtcgagcat cccttcatcc atgttgatat
    44461 cgataatctg cgcaccgttt tccacctgct gacgcgccac atccagcgcc tcgctgtatt
    44521 tctcttcttt gatcaggcgc ttgaacttag cggaaccggt gacgttggtg cgttcaccca
    44581 cgttcacaaa caggctatct tcgccaatgt tcagcggctc caggccggac aaacggcagg
    44641 ctacggggat ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgcgg
    44701 caatatgttg tggcgtggtg ccacagcagc cgccgacgat attgagaaag cccgcttgcg
    44761 cccattcacg tatctgtttt gccatcgtgt cggcgtcgag atcgtactca ccaaaggcgt
    44821 tgggtagccc ggcgttcggg tgcgctgtga cgtagcattc cgcaatccgt gataattcct
    44881 gcacgtactg gcgcagttcg tctggcccca gcgcacagtt aaggccgaaa gtcagcgctt
    44941 cggcgtggcg cagagagtta taaaacgctt cggtggtctg cccggagagc gtgcgcccgg
    45001 aggcgtcggt gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact
    45061 ccgttttcac cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca
    45121 gaatcaggtc ggcaccacct tccaccagcg ctttggtgga ctctcgataa gccgccacca
    45181 gcccgtcaaa agtgatatta cgaaatgccg gatcgttgac atccggagaa atagacgccg
    45241 tgcggttggt cgggccgaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg
    45301 tccactcatc agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca
    45361 ggaattccat ctggtaatcc gccatcgcaa tggttgtgga gttgaaggtg ttggtttcga
    45421 tgatatccgc gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttgc
    45481 tgagcaccag caggtcgttg ttgcctttga gatcgcatgg ccagtcggca aagcgttcac
    45541 cacgaaaatc ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca
    45601 gcaccagaat acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg
    45661 ctcccgacac gctcaaccca gatttaataa aaattcaaca aaccatactg gcataaacgc
    45721 atctgtggta aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat
    45781 cagttcagca actattgcat tagctaacaa taaaaatgaa aatgatttcc acgatacaga
    45841 aaaaggagac tgtcatggtc gcacccattc ccgcaaaacg cggcagaaaa cccgccgttg
    45901 ccaccgcacc agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt
    45961 ggattgccga atccaatggc agtgtggcac tcacggaact ggcacagcaa gccgggttac
    46021 ccaattccac gacccaccgc ctgctaacca cgatgcagca acagggcttt gtccgtcagg
    46081 tcggcgaact gggtcactgg gcaatcggcg cacacgcctt tatggtcggt agcagctttc
    46141 tccagagccg taatttgtta gcgattgttc accctatcct gcgcaatcta atggaagagt
    46201 ctggcgaaac ggtcaatatg gcggtgcttg atcaaagcaa tcacgaagcg attattatcg
    46261 accaggtaca gtgtactcac ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga
    46321 tgcacgcttc cggtgcgggt aaagcctttc tggcccaact gagcgaagaa caggtgacga
    46381 agctgctgca ccgcaaaggg ttacatgcct atacccacgc aacgctggtg tctcctgtgc
    46441 atttaaaaga agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac
    46501 atgcgctggg gctgcgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg
    46561 ccgcaatttc aatttccgga ccgatttcac gtattaccga tgaccgcgtg accgagtttg
    46621 gcgcgatggt gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atacgctgac
    46681 tttttcgcct ctgatttata cgaatcgaac gctaaaccgc tgcttgcgcc gatacgcata
    46741 aacatcttcc acatgccctt cacgtatgcg gttttgtagt gcgcgccagt aatcagcgcg
    46801 gaacaagtcg gcgtgcatct cttcaaatag cggaccaata cgcgggtcgg cacataacca
    46861 gtgacggaac tcttccggga aaacatcgcc cggcgagacg ctgtaccacg gttcgctggc
    46921 aagttcgtcc tccgggtagc gcggcggcgg gatgtcgcgg aagttcactt ccgtcatgta
    46981 gcaaatttcg tcgtaatcat aaaacaccac gcgcccgtga cgggtgacac cgaagttttt
    47041 aaacagcatg tcgccaggga aaatattagc ggcggcaagc tggcgaatag cgttaccgta
    47101 ttcttcaatg gcatcgcgca actgctgacc ttccacttgt tccagccaga tattgagcgg
    47161 caccatccgc cgctcaatat aaagatggcg aatcacaatt tgttcgccga gatcggtgat
    47221 tttttccgct gcttcctgga gcagtaatgc cattaatgcc ggggaaatat gccgcttctc
    47281 cagcacaaag ttttcaaact cctgggtgtc cgccattcgg cccacgcgat cgtgctcttt
    47341 caccagttga tagcaggcac gaacgtgagc ggcagacatc tctttctgcg gcgcgaactt
    47401 gtctttgatg actttgaata cccgatcaaa gcccggcagc gtaaacacca gcatcaccat
    47461 gccacgaata cccggcgctt caatgaactg ctcattacag ccctgtagat aaacgagata
    47521 ttcgcggtag ctttcggttt tggcgtgctt ctggcagccg atagccatat acaattcagc
    47581 ggtggtttta cctggcagaa tttcccgtag ccactcgacc agcgctgcgg gcagcggtgc
    47641 ataaaccata aaataagaac gcgcaaagcc aaaaacaatg ctcgcttcgg cggtggtcgt
    47701 caggcaggta tcaataaata actcgccgtc gtccgtctgg tggatcggca gcaaaaatgg
    47761 caatgtgccg gaaggcgtga tcagattgcc taccagccag gcggctttat tgcggtaaaa
    47821 cagttcgttc gccacctgta aatgactttc cgcgaggttg tctgtcccca gcgtttccgt
    47881 cagatggcga acgatgtaat gaatgtcacg gcttttattc tgccaacgca gacgcagcgg
    47941 cagatcgctg ataacgcgca tcagcagaga ttcccagccg tgatcggggt gaaagtcttt
    48001 cgccagcggg cggggaatgg tacgaaaacg gcgctctggc tgggagctaa agataaaaag
    48061 ccgctcggga gtaagcgagc ggtggtcaaa taacctacag tacacggagt tgaaaaagct
    48121 ctccgcaatc tcgaagcgcg ggtaatccgg caacagccgc gtgtaatgct ctttgacgcg
    48181 cagtaaaaat gccgcgtccg tgctttggcc gttggtaatg cagcgcagtt gctccacgac
    48241 cagacccacg tgatgatcgt aaagatgaat acggtttttc atcgcctgct ggacagcatg
    48301 ccagtcggct tgctcgaaac gctgctgcgc cccggaagtc acttcgagga atcgaccata
    48361 ctgagcgtcg aaaccttgca aaatggtttg tgcaatcagt aattccaggc cacgcggcat
    48421 ttgttgccct cgtcgggagc agagaattgc cggataagac gcgtaagcgt cacatcaggc
    48481 gctgattgcc ggatgcggcg tagacgcctt atccggccta cgttcggcaa cggctgtagg
    48541 catgataaga cgcgcaagcg tcgcatcagg cgctgattgc cggatgcggc gtgaacgcct
    48601 tatccggcct acagtcagca acgtttatcg gttgcttaga actgcgattc ttcggtggag
    48661 ccagtcagtg cggtaaccga cgacgtgccg ccctgaataa tggtcgtcac tttatcgaag
    48721 taacctgtac ccacttcctg ttggtgagat acgaaggtat agccatcttt cgcggcggca
    48781 aattccggct gctgcacttt ctcaacatag tgcttcatac cctcgccctg ggcataggcg
    48841 tttgccaggt caaacatgtt gaaccacatg ctgtggatac ctgccagggt gatgaactgg
    48901 aacttgtagc ccatatccga cagctgctgc tggaagctgg caatagtttt gtcgtcgagg
    48961 tttttctgcc agttgaacga cggcgagcag ttataagcca gcagtttgcc cggatatttc
    49021 gcgtggatgg cttgtgcaaa gcgacgcgcc agttccaggt ccggcgtgga ggtttcacac
    49081 cagaccaggt cagcatatgg cgcatacgcc aggccacggc tgatcgcttg ctcaataccc
    49141 gcatgagtac ggaagaagcc ttcactggta cgctcgccag taataaattc gctgtcatat
    49201 gggtcgcaat cggaggtaat cagatccgcc gcatcggcat cggtacgagc aaccagcagg
    49261 gttggaacgc ccgtcacatc agctgccaga cgcgccgcga ccagtttctg aatagcttcc
    49321 tgagttggca ccaaaacttt gccgcccatg tgaccacatt tcttcactga tgccagctga
    49381 tcttcgaagt gaactgccgc tgcaccggct tcaatcatcg ctttcatcag ttcaaaggca
    49441 ttcagtacac cgccaaaacc ggcctccgca tcggcgacga tcggcaggaa gtaatcgaca
    49501 tagcgcggat cgcccggctc aatgcccgcg gaccattgga tctgatccgc acggcggaaa
    49561 gtattgttga tccgctccac caccgctggc accgagtttg ccggatagag cgactgatcc
    49621 ggatacatgc tggccgccag gttagcgtcc gccgctacct gccatcccga cagatagact
    49681 gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc cagtcagtgc accgaggctg
    49741 ttgatgtagc cttttttcga ctcgccgtgc agcagacgcc acattttcgc cgcgcccagt
    49801 tgcgccaggg tgcattccgg attgactgaa ccgcgtaatt tcaccacatc ttccgcgctg
    49861 tacgggcgag taatgccttc ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt
    49921 tgttgtgtac gggttttcat gtgcagatgc tccatagtta tgtggtggtt tacgctaaca
    49981 ggcggtagcc tggcagggtc aggaaatcaa ttaactcatc ggaagtggtg atctgttcca
    50041 tcaagcgtgc ggcatcgtca aaacgcccct gggagaaacg ttcttcgccc agttcgctgg
    50101 caatgacttt catctcttcg cccagcatct ggcggaacaa ggctttagtc accggtttgc
    50161 cattgctcaa cgttttttga tgatggatcc actgccagat tgaggtacgg gaaatttcag
    50221 ccgtcgccgc atcttccatc aggccataaa tcggcacaca gccattgcca gagatccacg
    50281 cttcgatgta ctgcacagcc acgcgaatgt tggcgcgcat accttcttcg gtgcgttcac
    50341 catcacaagg tgccagcagc tgatcggcag taatcggcgc gtcttgttcg cgcatcactt
    50401 caagctgatt tttacgggag ccgagaatgt cgttgaatac cgccattgcc gtatccgcaa
    50461 ggcctgggtg ggcaatccat gtgccatcgt gaccgttatt agcttccagc gatttatccg
    50521 cttttacttt gttgagcacc tggttattgc gctcttcatc tttgctcgga ataaatgccg
    50581 ccataccgcc catcgcaaaa gcaccgcgtt tatggcaggt tttaatcaac aggcgtgagt
    50641 aagcattcag gaagggttta tccatcgtca ctgcctgtct atctggcagg acgcgatcgg
    50701 gatagttttt caatgttttg atataactga agatgtaatc ccaacgaccg cagttcagac
    50761 caacaatatg gtcacgcagc gcgtgaagga tttcatccat ctggaacacg gcgggcagcg
    50821 tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag attaaagcga tcttctgcat
    50881 agctgaagac ttcgctccac caggccgctt cctgccagga ctgggttttc ggcagataga
    50941 aatagggacc gctgcccttt gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa
    51001 aatcaaacag gctaccgggg attgcctcac cacgccaggt gacatgtttt tccggcaagt
    51061 gcagaccgcg tacccgacaa atcaaaaccg ctggattggg cttgagctga taaattttgc
    51121 ctgcttcatt ggtatagctg atggtgccgt taaccgcatc gcgcaggtta atttgcccat
    51181 cgatcacttt gttccagtct ggtgccagtg aatcttcgaa atcggccata aagactttca
    51241 cattggcgtt gagcgcgttg atcaccatct tgcgctcaac cgggccggta atctctaccc
    51301 gacgatcttc taagtccgca ggaatcccgc gaattttcca gtcagcatca cgaatggaag
    51361 ctgtttccga aataaaatca ggcaacgttc cgttatcgat atcctgctgc tgctgaatgc
    51421 gcgctgccag aagtttattg cgttgtggcg taaaatgcgt caccagctca gtcagaaatt
    51481 ctaccgcttc ggcagtaaga atatgcttct cctgctcgcc atacggcctt gtgaaagcca
    51541 attcatcggt tgttgttgcc tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa
    51601 cttccccact gtgaacgaag gatcgttgtg cgcttttcgt tcagcacaac taaga
//
LOCUS       DAZGAP010000008        51303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGAP010000008 DAZGAP010000000
VERSION     DAZGAP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.008"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(176..1087)
                     /gene="gsiD"
                     /locus_tag="ACHLAM_000404"
     CDS             complement(176..1087)
                     /gene="gsiD"
                     /locus_tag="ACHLAM_000404"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO7851994.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1090..2010)
                     /gene="gsiC"
                     /locus_tag="ACHLAM_000405"
     CDS             complement(1090..2010)
                     /gene="gsiC"
                     /locus_tag="ACHLAM_000405"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO7851995.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2028..3566)
                     /gene="gsiB"
                     /locus_tag="ACHLAM_000406"
     CDS             complement(2028..3566)
                     /gene="gsiB"
                     /locus_tag="ACHLAM_000406"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO7851996.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3586..5457)
                     /gene="gsiA"
                     /locus_tag="ACHLAM_000407"
     CDS             complement(3586..5457)
                     /gene="gsiA"
                     /locus_tag="ACHLAM_000407"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO7851997.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYRGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVVAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5444..6409)
                     /gene="iaaA"
                     /locus_tag="ACHLAM_000408"
     CDS             complement(5444..6409)
                     /gene="iaaA"
                     /locus_tag="ACHLAM_000408"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7851998.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6613..7848
                     /gene="moeA"
                     /locus_tag="ACHLAM_000409"
     CDS             6613..7848
                     /gene="moeA"
                     /locus_tag="ACHLAM_000409"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO7851999.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7848..8597
                     /gene="moeB"
                     /locus_tag="ACHLAM_000410"
     CDS             7848..8597
                     /gene="moeB"
                     /locus_tag="ACHLAM_000410"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO7852000.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCGVCGQ"
     gene            complement(8673..9335)
                     /gene="fsa"
                     /locus_tag="ACHLAM_000411"
     CDS             complement(8673..9335)
                     /gene="fsa"
                     /locus_tag="ACHLAM_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7852001.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9466..10365
                     /gene="ybiY"
                     /locus_tag="ACHLAM_000412"
     CDS             9466..10365
                     /gene="ybiY"
                     /locus_tag="ACHLAM_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO7852002.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10371..12803
                     /gene="ybiW"
                     /locus_tag="ACHLAM_000413"
     CDS             10371..12803
                     /gene="ybiW"
                     /locus_tag="ACHLAM_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO7852003.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12949..13764
                     /gene="ybiV"
                     /locus_tag="ACHLAM_000414"
     CDS             12949..13764
                     /gene="ybiV"
                     /locus_tag="ACHLAM_000414"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO7852004.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            13916..15175
                     /locus_tag="ACHLAM_000415"
     CDS             13916..15175
                     /locus_tag="ACHLAM_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO7852005.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(15416..17008)
                     /gene="ybiT"
                     /locus_tag="ACHLAM_000416"
     CDS             complement(15416..17008)
                     /gene="ybiT"
                     /locus_tag="ACHLAM_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO7852006.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIIGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17227..18147
                     /gene="ldtB"
                     /locus_tag="ACHLAM_000417"
     CDS             17227..18147
                     /gene="ldtB"
                     /locus_tag="ACHLAM_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7852007.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18205..19323)
                     /gene="ybiR"
                     /locus_tag="ACHLAM_000418"
     CDS             complement(18205..19323)
                     /gene="ybiR"
                     /locus_tag="ACHLAM_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO7852008.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19320..19787)
                     /gene="mntR"
                     /locus_tag="ACHLAM_000419"
     CDS             complement(19320..19787)
                     /gene="mntR"
                     /locus_tag="ACHLAM_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO7852009.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            19973..20101
                     /gene="mntS"
                     /locus_tag="ACHLAM_000420"
     CDS             19973..20101
                     /gene="mntS"
                     /locus_tag="ACHLAM_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO7852010.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20373..21956
                     /gene="opgE"
                     /locus_tag="ACHLAM_000421"
     CDS             20373..21956
                     /gene="opgE"
                     /locus_tag="ACHLAM_000421"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7852011.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22005..22520)
                     /gene="ompX"
                     /locus_tag="ACHLAM_000422"
     CDS             complement(22005..22520)
                     /gene="ompX"
                     /locus_tag="ACHLAM_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO7852012.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22573..22638)
                     /locus_tag="ACHLAM_000423"
     CDS             complement(22573..22638)
                     /locus_tag="ACHLAM_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852013.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            22873..23760
                     /gene="rhtA"
                     /locus_tag="ACHLAM_000424"
     CDS             22873..23760
                     /gene="rhtA"
                     /locus_tag="ACHLAM_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO7852014.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24060..24563
                     /gene="dps"
                     /locus_tag="ACHLAM_000425"
                     /gene_synonym="pexB"
     CDS             24060..24563
                     /gene="dps"
                     /locus_tag="ACHLAM_000425"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO7852015.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            24967..25713
                     /gene="glnH"
                     /locus_tag="ACHLAM_000426"
     CDS             24967..25713
                     /gene="glnH"
                     /locus_tag="ACHLAM_000426"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO7852016.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            25852..26511
                     /gene="glnP"
                     /locus_tag="ACHLAM_000427"
     CDS             25852..26511
                     /gene="glnP"
                     /locus_tag="ACHLAM_000427"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO7852017.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            26508..27230
                     /gene="glnQ"
                     /locus_tag="ACHLAM_000428"
     CDS             26508..27230
                     /gene="glnQ"
                     /locus_tag="ACHLAM_000428"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO7852018.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            27347..29572
                     /gene="ybiO"
                     /locus_tag="ACHLAM_000429"
     CDS             27347..29572
                     /gene="ybiO"
                     /locus_tag="ACHLAM_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO7852019.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(29569..30495)
                     /gene="rlmF"
                     /locus_tag="ACHLAM_000430"
     CDS             complement(29569..30495)
                     /gene="rlmF"
                     /locus_tag="ACHLAM_000430"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO7852020.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            30771..31031
                     /gene="mcbA"
                     /locus_tag="ACHLAM_000431"
     CDS             30771..31031
                     /gene="mcbA"
                     /locus_tag="ACHLAM_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO7852021.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            31296..33578
                     /gene="fiu"
                     /locus_tag="ACHLAM_000432"
     CDS             31296..33578
                     /gene="fiu"
                     /locus_tag="ACHLAM_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO7852022.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIKNEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            33620..34297
                     /gene="ybiX"
                     /locus_tag="ACHLAM_000433"
     CDS             33620..34297
                     /gene="ybiX"
                     /locus_tag="ACHLAM_000433"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO7852023.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            34371..34637
                     /gene="ybiI"
                     /locus_tag="ACHLAM_000434"
     CDS             34371..34637
                     /gene="ybiI"
                     /locus_tag="ACHLAM_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO7852024.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            34902..35162
                     /gene="ybiJ"
                     /locus_tag="ACHLAM_000435"
     CDS             34902..35162
                     /gene="ybiJ"
                     /locus_tag="ACHLAM_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO7852025.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(35302..36387)
                     /gene="hcxB"
                     /locus_tag="ACHLAM_000436"
     CDS             complement(35302..36387)
                     /gene="hcxB"
                     /locus_tag="ACHLAM_000436"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO7852026.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAVVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(36528..37490)
                     /gene="ybiB"
                     /locus_tag="ACHLAM_000437"
     CDS             complement(36528..37490)
                     /gene="ybiB"
                     /locus_tag="ACHLAM_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO7852027.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIKRCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            complement(37518..39668)
                     /gene="dinG"
                     /locus_tag="ACHLAM_000438"
     CDS             complement(37518..39668)
                     /gene="dinG"
                     /locus_tag="ACHLAM_000438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO7852028.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            39772..39972
                     /locus_tag="ACHLAM_000439"
                     /pseudo
     CDS             39772..39972
                     /locus_tag="ACHLAM_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            complement(40207..41568)
                     /gene="rhlE"
                     /locus_tag="ACHLAM_000440"
     CDS             complement(40207..41568)
                     /gene="rhlE"
                     /locus_tag="ACHLAM_000440"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO7852029.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            41797..42468
                     /gene="cecR"
                     /locus_tag="ACHLAM_000441"
     CDS             41797..42468
                     /gene="cecR"
                     /locus_tag="ACHLAM_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO7852030.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            42471..43466
                     /gene="hlyD"
                     /locus_tag="ACHLAM_000442"
     CDS             42471..43466
                     /gene="hlyD"
                     /locus_tag="ACHLAM_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO7852031.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            43459..45195
                     /gene="ybhF"
                     /locus_tag="ACHLAM_000443"
     CDS             43459..45195
                     /gene="ybhF"
                     /locus_tag="ACHLAM_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7852032.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            45188..46321
                     /gene="ybhS"
                     /locus_tag="ACHLAM_000444"
     CDS             45188..46321
                     /gene="ybhS"
                     /locus_tag="ACHLAM_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7852033.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            46332..47438
                     /gene="ybhR"
                     /locus_tag="ACHLAM_000445"
     CDS             46332..47438
                     /gene="ybhR"
                     /locus_tag="ACHLAM_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7852034.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(47400..47810)
                     /gene="ybhQ"
                     /locus_tag="ACHLAM_000446"
     CDS             complement(47400..47810)
                     /gene="ybhQ"
                     /locus_tag="ACHLAM_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO7852035.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            47943..48704
                     /gene="ybhP"
                     /locus_tag="ACHLAM_000447"
     CDS             47943..48704
                     /gene="ybhP"
                     /locus_tag="ACHLAM_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7852036.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKMQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            48701..49942
                     /gene="clsB"
                     /locus_tag="ACHLAM_000448"
     CDS             48701..49942
                     /gene="clsB"
                     /locus_tag="ACHLAM_000448"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO7852037.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAMFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            49942..50898
                     /gene="ybhN"
                     /locus_tag="ACHLAM_000449"
     CDS             49942..50898
                     /gene="ybhN"
                     /locus_tag="ACHLAM_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO7852038.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(50934..>51303)
                     /locus_tag="ACHLAM_000450"
     CDS             complement(50934..>51303)
                     /locus_tag="ACHLAM_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852039.1"
                     /translation="SNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRNAALMSER
                     PIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLYYYFIGFF
                     GILAAIAITLVWQRHTRFFH"
BASE COUNT        12298 a        13762 c        13057 g        12186 t
ORIGIN      
        1 ttaatacatt taacaaaata actgtctgaa cgctgtgccg ctgcaaaata gtcccttgct
       61 gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac aacacttgct
      121 tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa cgtaactatc
      181 ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg aacgccagta
      241 cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata accatatccg
      301 ctcgtgcctc attgagcatt gcaccccact ctggtgtcgg cggctgcgca ccgaggccga
      361 gaaatgacag gctggcggca gagataatcg aggcgccaat gcgcatggtg aaaaacacca
      421 cgatagaaga gacggtccca ggcaagatat gacgcaacaa aatggtcata tcgctggcac
      481 caatactgcg tgctgactcg ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca
      541 ccagccgggc aaacgcgggg atggaaaaaa tggcgactgc gataatcacg ttagcaatgc
      601 cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag gcaaacaaca
      661 catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca gccagcaagc
      721 ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc gccagcgata
      781 tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg acaccaaacc
      841 agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt tcggcgtcat
      901 agggagcgat ccagcgtgca aaaatggcca ccacaatcaa taaaataacg aacaatgcgg
      961 cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgta
     1021 cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata
     1081 gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca catccaccac
     1141 taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta
     1201 atcgcgcatt tcaacggagt caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt
     1261 ctccaccacg atggaaccac cgagtaaaaa tccaaactgt aagcccatca tggtcactac
     1321 cgggatcatc gcgttgcgta gcccgtgttt gaggacaacc caggtttcgc tcaccccttt
     1381 cgccctcgcg gtacgcatat aatcttcgct taaaacgtcg acaaacgacg cgcgggtaaa
     1441 gcgcgccatc acggcggcca ctgccgcgcc gagcgtcagg gagggtaaaa tgtagtgctg
     1501 ccaactgtct gctcccacgg taggcagcca gcccagttca acagagaata cctgaattaa
     1561 aagcatcccc agagcaaatg ccggaaacga aatccccgac accgcaatag tcatactcaa
     1621 tcgatccggc caacggttac gccagacggc ggcgataatt cccgccgcca taccaaatat
     1681 caccgcccag accatactgg ttatggtcag ccacagcgtt ggcataaagc ggctggcaat
     1741 ctcatcgcca accggacgac gcgacaccat cgacaggcca aaatccccct gcacagcatt
     1801 gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct gctgacgcac
     1861 cagttctata acctgcgcat cagcttcggg cccggcaatt aatcgcgccg gatcgccggg
     1921 cagcatgtgg acaaataaaa acaccagcac cgagacgata aacagcgtcg gaatcaaccc
     1981 cagtaagcgt ttgataacgt aattaagcat tccactccct gcgttgctta ttgcaaatcc
     2041 gcatcttcaa agctgaagcc ggtgtctggc atgatccaaa aaccggtcag gtttttactg
     2101 tgtgccgaca caagtttttc taccaccagc gggatccacg gcgactcttg ccagatgata
     2161 tcctgcgccg ccttatacaa gcgggtcttt tccgccggat cattagtttt cagtgcctga
     2221 gccaggaagt catccacctg tttattgctg taaaacgcgg tattaaacag cgttggtggc
     2281 cagttctgcg aggcaaacag cggcgataga gcccagtcgg cttcgccggt tgaagccgac
     2341 cagccagtgt agaacatccg cacgccgctc tctttttgcc ctttaccttc aacttctgcc
     2401 gcccgctgtc cggcatccat cgcagtcacc tgggctttaa tcccaacctg cgctaactgc
     2461 tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg
     2521 gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg
     2581 taaggccacg gtttataact ttgcgcgtag gcgatactcg gcggtaccac accagtagct
     2641 ggcgttgcat agcccgcgaa ggccactttc accaacgccg ggcggttaat ggcgtaattc
     2701 agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat actgatataa
     2761 cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc cagcagtgcg
     2821 gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg
     2881 cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg caatcctggc
     2941 tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca ggtatccagt
     3001 tcatacggcc cggttcccac cggatgaaaa ccaatctcct tgccatattt ttccagcgct
     3061 gccggtgaaa tcatcgcggt cgccggatgg gcaagaatat taataaacgc tgagaatggc
     3121 tgtttgaggg taatctttac cgttgtcgga tcgatcgctt ccgttttggc aatattctta
     3181 tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag attcgctttt
     3241 accgccgctg cgttgaagtc ggttccatcc tggaatttaa tgccttcccg cagtttcacg
     3301 gtgtaagtaa ggccgtcatc ggaaacggta taactctccg ccagcacgtt tttcagtttc
     3361 atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc ctgagataac
     3421 gtgtcattcg cgtcatatgg atcgagcgtg gtgaagttcg atcctaccgc caccaccaca
     3481 tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag cgccactaac
     3541 ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc tacgcatgaa
     3601 tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt gcaacgagac
     3661 ggctacaacc tcttcaccac gcagatgaat attgctggga agatcgtccg acagcagtac
     3721 acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta atttacgcgt
     3781 ataaggatgc tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa caatttgtcc
     3841 gcgatacatc accgccacac gatgactaat ccgctctacc acggccatat cgtgggagat
     3901 aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga taatctgccc
     3961 gcgaatagaa acatccagcg ccgagacggc ttcgtcggca atgatcactt ttggattcaa
     4021 tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact catgagggta
     4081 acgccaggca tgttccggta acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc
     4141 cgcttcttta cctggcaata aaccgtgtac gcgcagcggt tcgataatcg aatcaccgat
     4201 ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg
     4261 caaagcctga agtttaccgg atgacaaggt atcgattcgc tgaccgttaa agataatttc
     4321 gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc
     4381 agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga ctttctcaac
     4441 ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac
     4501 cagattacgc acccgtaaaa caggttcgcc atccaccacc gttttctgct cgatgggggg
     4561 ttcctgtttt gctggatgtt caagcgatat caatgggaaa cgtcggggat aatctaaccc
     4621 tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtataag gatgttgcgg
     4681 tgcatgaaaa atctgttcga cgctacccgt ttccaccgcc tcgccctgat acatcaccag
     4741 tacccgatcg gcaatctctg ccaccacgcc catatcgtga gtgataaaga taacgcccat
     4801 cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc
     4861 caacgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca gcgccatcgc
     4921 aatcatcact cgctggcgca tcccgccaga gagttgatgc ggataacgtg aaaggatggt
     4981 ttgtgcctcc ggaatgcgta cctgatccag catccgcttc gcctcgacca tcgcttcttc
     5041 acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca cagtaaatac
     5101 cgggttcagc gatgtcatcg gttcctgaaa tatcatcgcc atatccgcac cgcgcacatg
     5161 gcgcatttgt gcagcgctct gctcgctaag ttcaatcact tcacgactgc gccgccgcaa
     5221 cagcatttga tcgcactgca ctaaaccgcc cgcctgttcc aacaagcgca tcaatgccag
     5281 cgcagtcact gacttaccag agccggattc gccaacaatt gccagcgtct caccgcgttg
     5341 caggctaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca taaaggcaat
     5401 attcagattt tcaaccgcca gcacatcacc ggcatcaagt tcatcactgt gtggcaacgg
     5461 tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc
     5521 gatacattcc ttcggtgtta aacggtagcg cgacgttacc ttcatggtcg atagcgatta
     5581 agccaccgct accgccaagc gcaggaagtt tttccattac tacccgctcg caggcttccg
     5641 cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc agcgcgcgga
     5701 tgaagacttc gcccgtgccg gtacaagaaa ccgccacact ggcgttattg gcgtagcatc
     5761 cggcacccac taaggggcta tcgccaactc gtccgggtaa tttattggtc attccgcccg
     5821 tggacgtagc tgccgccaga ttgccgtcta aatccaacgc cacggccccc acggtgccca
     5881 ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag aaccattgcc ccttcctcgc
     5941 gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatctccggc gagacgcgct
     6001 ccatgccatg agcaaacgca aaattttctg ccccttcgcc aatcatcatc acatgcgggc
     6061 tttgctccat caccagccgg gcggcaagaa ccggattacg cagatgacta acgcctgcca
     6121 ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca tgggtttcat
     6181 cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc agcagacgca
     6241 ccgcttccgt caccacatcc agcgcacttt cgcccgcttc cagcattttc tgcccggttt
     6301 caacaatggc agacagcgcc tcgatgtagc gtaattcctg ttgcagactc atctgcgcgc
     6361 ggctaattgc acctgcgccg ccatgaattg caatgactgc tttgcccata gtattcgtcc
     6421 attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat tactcattca
     6481 atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg ctacgcccga
     6541 ggtggcatac agtctgtggc gtttttctga cataataggc aaattcgatt ttgcctccgc
     6601 aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag
     6661 atgctttctc gcgttacccc actgaccgcc caggaaacgc tgccactggt tcagtgtttt
     6721 ggtcgtattc tggcgagcga tgtcgtttcg ccactggatg tcccggggtt tgataactcc
     6781 gctatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt
     6841 gccggtaaat cctttgccgg tcagccgtac catggtgaat ggcctgcggg tacctgcatt
     6901 cgtattatga ccggtgcgcc ggtgccagaa ggctgcgaag cggtggtgat gcaggagcag
     6961 actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt
     7021 cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt tcccggcggg gactcgcctg
     7081 actaccgctg aactgccagt gattgcttcg ctagggattg ccgaagttcc ggtgattcgt
     7141 aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg tcagccgctg
     7201 ggcgacggcc aaatctacga taccaaccgt ctcgccgtac acctgatgtt agaacagttg
     7261 ggatgcgagg taatcaactt agggattatc cgcgacgatc cccatgccct gcgcgccgca
     7321 tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt
     7381 gaagcggatt acaccaaaac gattcttgaa gagctggggg agatcgcctt ctggaaactg
     7441 gcgattaaac cgggtaaacc gttcgcgttc ggtaaacttt ctaatagttg gttctgcggc
     7501 ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca gcctttgctg
     7561 gcaaaactaa gcggcaatac cgccagtggc ctgcccgcgc gccagcgtgt acgcacagcg
     7621 tcccgcctga aaaaaacgcc aggacgcctt gatttccagc gcggcgtgct gcaacgcaac
     7681 gccgatggcg aactggaagt gacgaccacc ggacatcagg gttcacatat atttagctcc
     7741 tttagcctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt ggaagtgggc
     7801 gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg gcggaactca
     7861 gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt gattttgacg
     7921 gtcaggaagc attgaaagat tcccgcgtgc tggtcgtcgg ccttggtggg ctcggctgcg
     7981 cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc gacttcgaca
     8041 cggtttcgct ctccaatctg caacgccaga cgctgcacag tgatgccacg gtcgggcaac
     8101 caaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc gcgattacgc
     8161 cagtcaatgc gctgcttgat gacgctgaac ttgctgcgat gattgcaaaa cacgatctgg
     8221 tgctcgactg tacggataac gttgcagtac gtaatcaact gaacgcaggc tgttttgccg
     8281 cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca
     8341 cttatcagga cggcgaaccg tgctatcact gccttagccg tttgtttggc gagaacgcgt
     8401 taacctgcgt agaagcaggc gtgatggcac cgttaatcgg cgtaattggt tcgttgcagg
     8461 caatggaagc aatcaaactg ctggcaggtt atggcaaacc agccagcgga aaaatcgtga
     8521 tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgcaat ccggggtgtg
     8581 gggtgtgcgg gcagtaactg ccacctccag gccagataag acgcggcact ccgccacgtc
     8641 atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc
     8701 agccagtcct gttcaaactt cgccacagcg gcatcaaccg ccggatagct aatcatctgt
     8761 tgtgccacat ccagcggcag agtaattgat tcacatcctg ccagtaagca gtccagcgcc
     8821 tgacgcgggg ttttgaaact cgcggctaac actttcgcct gcggcgcatg cattttcaat
     8881 aactggtgta agtcggtcac agtctgaata ccgctaccgc cctgagcatc aatacgatta
     8941 acgtaaggcg caacatattc cgcacctgcc agcgccgaca gtagcccttg tgctgcgcca
     9001 tataccgcgg ttcccagcgt cggaatccct tccgctttta acatcttaat agctgccagc
     9061 ccctcagcgg tcaccggaac cttcaccacg atatccgcaa taatagaacg caatttacgt
     9121 gcgtcattaa ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga
     9181 ccgcccatcg cctcgtgaag ttgcggaagc acaacctcca gcggtttttt acccgctgcg
     9241 ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgtcttcacc
     9301 gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg
     9361 tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatctaactt
     9421 tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt
     9481 caacgctact cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt
     9541 tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg
     9601 tatgacccac gactctgtct ggaaggctgt gagctgtgcg ctaagaccgc accggaagtg
     9661 attgaacgcg cgttgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatttt
     9721 acggcgctaa ccgactgctg cccgacacag gcgttaaccg tgtgtggtga agtgaaaagc
     9781 gttgaggaga tcatggcaac cgttttgcgc gataaaccgt tttacgatcg cagcggcggc
     9841 ggcttaacgc tttctggcgg tgaaccgttt atgcagccgg aaatggcgac ggcgctgctg
     9901 caagccagcc acgaggccgg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg
     9961 aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacgtt
    10021 gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccagggtgct ggataacctg
    10081 aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccactgat tcagggcttt
    10141 aatgccgacg aaacctctgt gaaagccatt accgattttg ccgccgacga gctgcacgtt
    10201 agcgaaattc atttcctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat
    10261 ctgccctatg acgccccgga aaaaccgctt gatgcgccag aactgctcga ctttgcccag
    10321 cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaatc atgacaacac
    10381 taaaactgga tacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg
    10441 tgaaaccgcc ggtctgtacc gaacgagcac aacactatac cgagatgtat caacaacatc
    10501 tcgataagcc aatcccggta cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca
    10561 ccatctggat caaacatgat gaattgatca ttggcaacca ggcaagcgaa gttcgcgccg
    10621 cgccgatctt cccggaatat accgtctcct ggatcgaaaa agagattgat gatctggcag
    10681 atcgtcccgg tgctggtttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt
    10741 gcccgtggtg gcgcggtcag accgtacagg atcgctgcta cggcatgttt accgatgagc
    10801 aaaaaggtct gctggcgacc ggcatcatta aagcggaggg caatatgacc tccggcgatg
    10861 cgcacctggc ggtcaatttc ccgctactgc tggaaaaagg tcttgatggt ctgcgtgaga
    10921 aagtggcgga acgtcgctcg cgcatcaatc tgacggttct ggaagatctg cacggcgagc
    10981 aattcctgaa agcgattgat atcgtgctgg tggcagtcag tgaacacatt gaacgtttcg
    11041 ctgccctggc gcgtgaaatg gccgcaaccg aaacccgcga aagccgtcgc gatgaactgc
    11101 tgacgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg
    11161 cgctgcaact gtgctacttc atccagttga ttttgcagat cgaatctaac ggtcactcgg
    11221 tatcgtttgg tcgtatggac cagtatctct acccatacta tcgccgtgac gttgaactga
    11281 accagacact ggatcgcgaa cacgccatcg agatgctgca tagctgctgg ctgaaactgc
    11341 tggaagtgaa caaaatccgc tccggctcac actcaaaagc ctctgcggga agtccgctgt
    11401 atcagaacgt caccattggc gggcaaaatc tggttgatgg tcaaccgatg gacgcggtaa
    11461 atccactctc ttatgcgatc ctcgaatctt gcggtcgcct gcgttcgact cagcctaacc
    11521 tcagcgtgcg ttaccatgcg ggtatgagta acgatttcct cgacgcctgt gttcaggtga
    11581 tccgctgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta
    11641 ttaaactcgg tattgaaccg caggacgctt acgactacgc agcaattggt tgtatcgaaa
    11701 ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc
    11761 gcgtgatgct ggcggcgctg gaaggtggtc gtgatgccac cagcggcaaa gtgttcctgc
    11821 cacaagcaaa agcgttgtca gcgggtaact tcaacaactt cgatgaagta atggacgcat
    11881 gggatacgca aatccgttac tacacgcgta aatcaatcga aatcgaatat gtcgtcgata
    11941 ccatgctgga agagaacgta cacgatattc tctgctcggc gctggtcgat gactgtattg
    12001 agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg
    12061 tcggcattgc taacctcggc aacagcctgg cggcagtgaa gaaactggta tttgaacagg
    12121 gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgac ggcctgactc
    12181 acgagcagct gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata
    12241 ctgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg aagcagtacc
    12301 ataacccgcg ctacggtcgt ggtccgattg gcggcaacta ttatgcgggc acatcgtcta
    12361 tctccgctaa cgtaccgttt ggcgcgcaga cgatggcaac gccggacgga cgtaaagcac
    12421 ataccccgct ggcagaaggc gctagtccgg cctccggtac tgaccatctc ggccctactg
    12481 cggtcattgg ctcggtgggt aaactgccta cggcagcaat tctcggcggc gtgctgctca
    12541 accagaaact gaatccggca acgctggaga atgaatctga caagcagaaa ctgatgatcc
    12601 tgctgcgtac cttctttgaa gtgcataaag gctggcatat tcagtacaac attgtttccc
    12661 gcgaaacatt gctggaggcg aagaaacatc cggatcaata ccgcgatctg gtggtacgcg
    12721 tagcgggtta ttccgccttc ttcaccgcgc tctctccaga cgcacaggac gatatcatcg
    12781 cccgtaccga acacatgctg taatcccctc agcccggcgt cgtcgtcgcc gggccaaatc
    12841 attcacatca tcaatttcag cctcgctttc attcgaaata taatttgtgc tctgcgtcac
    12901 attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa
    12961 gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca
    13021 cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt tgtcgccagc
    13081 ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt
    13141 gtcgcggaaa acggcgcact ggtttacgaa catggcaagc agttgttcca cggcgaactg
    13201 acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt
    13261 gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc attcgtcgca
    13321 ctgatggcaa aacactacca tcgcctgaaa cctgtaaaag attatcagga gattgacgac
    13381 gtactgttca agttttcgct caacctgccg gatgaacaaa tcccgttagt gatcgacaaa
    13441 ctgcacatag cgctggatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac
    13501 ttgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat
    13561 ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa
    13621 atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt
    13681 tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat
    13741 aatatcgctc ctttcaatag ttgatcttcc ccctggccga agtgaccact tcggcctctc
    13801 tcctttttgc tcgctttgtg cactgactca atatttttat cttgcgttaa cttattttta
    13861 acttaatgta tcaatcgcaa tcattttact ttcgaatgaa agaagcgagg ttgatatgac
    13921 tttttcctgt gagacgctgc ccacagatgc gaaagcagct atccgagaga tgaaagctgc
    13981 gctgcgggaa cagctcagtg atgtgcaaca ggttttcgat acactctctg caaaaatcga
    14041 aacgcgtgtt gctgaaatcg atgccctaaa agcgcaaggg ctgccggttt ggccggaagt
    14101 ttcttatagt gatattgccg caggtacagt aagcgatgcc actcgcaatg aaattaaacg
    14161 tcgcggttgt gcagtcataa aaggccattt ccctcgcgag caggcgatgg cgtgggatcg
    14221 tgccatgctc gattatcttg atatcaatca ttttgatgat gtttataaag gccctggcga
    14281 cagttttttc ggctcgctgg aggcttctcg tcccgaaatc tatcccattt actggtcgca
    14341 agcacagatg caggcgcggc agagcgaaaa catggctgct gtgcaatctt ttctaaaccg
    14401 actgtggaag tctgaaagca acggcaaaca gtggtttaat cctgatatca gtgtgattta
    14461 tccagacagg atccgccgcc gccctccagg tactacatct aaaggcctgg gcgcgcatac
    14521 cgattctggt gcgctggaac ggtggttatt acccgcttat cagcgcgttt ttgccaatgt
    14581 gtttaatggt aatttcgatg attacgatcc ctgggatgct gcacaccgga ccgatgtcga
    14641 agagtacacg gtagataaca ctacaaaatg ttcggtgttc cgcacgtttc agggctggac
    14701 ggcgctctct gatatgttat cgggtcaggg attgttacat gtggtgccta tcccggaagc
    14761 gatggcgtat gttttattgc gcccgctgct ggatgacgta ccggacgatg aactgtgcgg
    14821 ggttgcacct ggcaaagttc tgcccatttc agagaaatgg catccactac tgcttaaagc
    14881 gctgacttcc attcccgcga taacagcagg ggattccgtc tggtggcact gcgatgtgat
    14941 tcactccgtc gcgccagtag acaatcaaca gggttggggc aacgtgatgt atatccctgc
    15001 tgcgccaatg tgcgagaaaa atcgggctta tgcacacaaa gtcaaaatgg cgctggaaaa
    15061 aggcgcatca cctggcgact tcccgcggga agattatgaa gcaaactggg aaggccgctt
    15121 tacgctggag gatctgaaca ttcacggtaa gcgcgcactg ggcatggatg tttgagagtt
    15181 aatggaagtg aattgatgtg ggattaagaa aatcgattca acgttactca gattgctgac
    15241 aaatgcgctt tgttaatgcc tgatgcggcg ttaacgcctt atccggccta caaagtcctg
    15301 cagattcaat atattgcagg tactgcgtag gcctgataag catagcgcat caggcaatct
    15361 tgcgtttgtc atcagccgcc gtcaccccga cagtatcggg ctgacggata ataacttact
    15421 cgatcccttt actacgcaga taatcttcgt aattaccgct aaagtcgatc acgcgttccg
    15481 gggtgatttc cagaattcgg gtcgccaggg agcttacaaa ctcacggtcg tgagacacaa
    15541 agatcagcgt gccctgatac agttccagcg ccatgttcag cgactcaatg gattccatat
    15601 ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg cttctgcatc attaacttac
    15661 caaacagcat ccgccctttc tccccaccgg aaagcacttt cgctggcttt ttgatgtcgt
    15721 cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc ctgctcgtca tcgccttcct
    15781 gcttccactg actcatccat tcgaacacgg taagatcatt ttcgaactca tattcgtggt
    15841 cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt tacggtgccg ctgtccggtt
    15901 gcagatcgcc caccagcgtt ttcaacagtg ttgatttacc gacgccgttg gtacccagta
    15961 ccgccagttt ttcaccgact tccagcagca ggttgagatt tttaaacagc ggaccgttat
    16021 caaacccttt ggtcagacct tccacttcca gcgcgttacg gaacagtttc ttatcctgtt
    16081 caaaacggat gaacgggttc tgacggctgg aggctttcac ctcttccagt ttgattttat
    16141 caatctggcg cgcgcgcgaa gttgcctggc gagatttcga ggcgttggca ctaaagcggc
    16201 taacgaaaga ttgcagctca gcaatctgcg ctttcttctt ggcgttatca gccagcagac
    16261 gctcacgcgc ctgggtcgcc gccgtcatgt actcatcgta gttacctgga taaacgcgca
    16321 gctcgccgta atccagatcc gccatgtggg tacaaaccat gttaaggaag tgacggtcgt
    16381 gcgagatgat gatcatggtg ctgtcacgct cgttcagcac ctgttccagc caacgaatgg
    16441 tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag aatgtccgga tcagcaaaca
    16501 gcgcctgcgc cagcagcaca cgcagcttcc agccaggagc aacttcactc atcgggccgt
    16561 agtgctgttc cactggaatt cccacgccaa gcaacagttc accggcgcga gcttccgcag
    16621 agtaaccgtc catttcgccg tatttcactt ccagatcggc cactttatag ccgtcttctt
    16681 cactcatttc cggcaaagca tagatgcggt cacgctcctg cttcacttcc cacaactctt
    16741 tatgcccaat gatcaccgta tccagcacag tgaactcttc aaaggcaaac tgatcctgac
    16801 gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt acccaacgtc ggctctaagt
    16861 cgccgccgag gatcttcata aaggtggatt taccactacc gttcgcgcca atcaggccgt
    16921 aacggttgcc gccgccaaat ttgacggaaa tgttttcaaa caacggctta ctgccgaact
    16981 gcatggtgac gttactggaa actaacacag gcgtatcctg aaaagagata tgacaaaccg
    17041 cgcattatgc cataaatctg aaagaagatc ctgctcctcg cttaaggtct acactcaagc
    17101 caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac tgtgagctgg aatgaactta
    17161 taatgcgctt ccaatactct aatattctca acccaatggc ctgccaggca caaaatctcg
    17221 cttaacatga atatgaaatt gaaaacatta ttcgcagcgg ccttcgctgt tgtcggcttt
    17281 tgcagtaccg cctctgcggt aacttatcct ctgccaaccg acgggagtcg cctggttggt
    17341 cagaatcagg tgatcaccat tcctgaaggt aacactcagc cgctggagta ttttgccgcc
    17401 gagtaccaga tgggactttc caatatgatg gaagcgaacc cgggtgtgga taccttcctg
    17461 ccaaaaggcg gtactgtact gaacattccg cagcagctga tcctgccgga taccgttcat
    17521 gaaggcatcg tcattaacag tgcagagatg cgtctgtatt actatccgaa aggaaccaac
    17581 accgttatcg tgctgccgat cggcattggt cagttaggca aagacacgcc tatcaactgg
    17641 accaccaaag ttgagcgtaa aaaagcaggc ccgacctgga cgccgaccgc caaaatgcac
    17701 gcagagtacc gcgctgcggg cgaaccgctt ccggctgtcg ttccggcagg tccggataac
    17761 ccgatgggtc tgtatgcact ctacatcggt cgcctgtacg ctatccatgg caccaacgcc
    17821 aacttcggta tcggcctgcg tgtaagtcat ggctgtgtgc gtctgcgtaa cgaagacatt
    17881 aaattcctgt tcgagaaagt gcctgtcggt actcgcgtgc agtttattga tgagccggtg
    17941 aaagcgacta ccgaaccaga cggcagccgt tacattgaag tccataaccc gctgtctacc
    18001 accgaagccc agtttgaagg tcaggaaatt gtgccaatta ccctgacgaa gagcgtgcag
    18061 acagtgaccg gtcagccaga tgttgaccag gttgttcttg atgaagcgat caaaaatcgt
    18121 tccgggatgc cggttcgtct gaattaatcg tcaaaacgta aaacaaaggc ggactcataa
    18181 tccgcctttt ttatttgcca gaccctacca ggccgggatc ataaccaaca aaacatatcc
    18241 caccagcgcc gcccacaaca gcatcggtat cgaataaaga tgaaaacgcc accagatacg
    18301 gcgatcgttc gccatccgta acgcaatcaa atttgccagc gatccgggta ataaaccaaa
    18361 gccccctacg tttaccgccc atgccagcag caacgacggc ggcacatagt tcagcaacaa
    18421 tatggtactc ggcacgttac tgatcacctg cgataaaccg attgccgtta accataatcc
    18481 gggttcggat aaatgactca cgttacttaa caccccctgc aaagctggca gttgggttag
    18541 taaatggacg tcgataaaca tcgccataaa caccagcagc agcgtccagt ccacactgag
    18601 caccacgcga cgtgccagca gcgcaaagcc caccgccaca atcaccagtc cccacagctc
    18661 ttgttttaac tccagcgcca tcagaaagac gatatacagc cccagacaac tccacaccag
    18721 ccgcggtttc cactccggcg tttgcgcccc cgtatggtat tgcagcgcct ttccagggaa
    18781 acaacaccag cacaacagca ggagtgtcag catcattgcg ccagccagcg gtgccatttg
    18841 ggcaataaat ccggcaaacg aaagaccaga acgtccccag ataagaatat tttgcgggtt
    18901 gccaattggc gtcagtagcg aaccagcgtt gactgccagc gcctcaaaaa taatcagccg
    18961 attaaccggg atctcacaca gtctttttag cgtgatagtc agcggaacaa caataaacag
    19021 cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc gccagcacca taaacatcgc
    19081 cagccgacgc tccgtagcaa agcggcgcac cattttgcgc cccagcacat caaaataacc
    19141 gcttaactcc acacctttgg tcagcagcat caggccgctt aaggtgatga tggtgtgcca
    19201 gtcgatagca gcaggccagg atttcggtgc aaagggaacg aaaaaactta atccgatgcc
    19261 aacaagaatt aataactgaa aaaaacgatc gccttgcagc gtgcgtaaaa aaggcaggct
    19321 catttggcac cgtgtttctg ggtaaacaaa cgaaaggcat ccagcgtctc ttcactaaca
    19381 tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg gactgacgcc caacaccaac
    19441 aagaaatttt cgactatctg atgtcgctcg cggctttcct gcgccagttt ctctccttct
    19501 gccgttaaaa acacgccacg ccaggggatc atttcaatca gccccatggt tgccagtcgc
    19561 ttaagcattt tagccaccgt cggttgcgaa actcccaaac gagcagccat gtctacctga
    19621 cgagcttccc ccacttccct gatcaagtca gaaatcagct caacgtaatc atcaatcagc
    19681 tcgcgccgat gcgcctctct gacctggcgg aacccttcaa cgtgctcttc cacgttcact
    19741 aactgcgtca ctttttttgc tgttggcgta cctgcgcgac gactcattgt gcttcctcat
    19801 tatggtgacg catacatagc gttctcattt cgtgcgcaca ttgtaaacca gagttgcgaa
    19861 ggtacaaaaa attaacgttt tagcaatagc tatataatat agcctgtgct atatctgtat
    19921 gtaatgcaat catccctcaa ggatcgacgg gattagcaag tcaggaggtc ttatgaatga
    19981 gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt aaagtacggt taatgctgct
    20041 ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa gataaacctt cagataaata
    20101 gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca ggcataacgc gttttggtct
    20161 gaaaaacccc actttttgtc ggatttgcaa tccccttcgc aaaagatttg ttcgtcagta
    20221 gttgacctga acggcggctc gctctatctt cttgcagccc tgcgtatatt gcggctcgcg
    20281 gatgcggacc cctttcccct ctccacgcac tcttgcaggt attgaccctt gacgccaagg
    20341 taagcacatg gcgtttgtta cgatagtggc atatgaattt aaccctcaaa gaatcgcttg
    20401 ttacccgtag ccgggtattt agcccgtgga ctgcgttcta ctttttacag tcgctattaa
    20461 ttaacctcgg cttaggttat cccttcagtt tgctctacac cgctgcgttt acggctattt
    20521 tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt tctggtcggt gtcagttcgc
    20581 tggtggcggc ttgttatttc ccttttgctc aggcctacgg cgcacctaat ttcaatacat
    20641 tgctggcatt gcactccacc aatatggaag agtcgaccga aatcctgacg atttttccgt
    20701 ggtacagcta cctggtcggc ttatttattt ttgcgctcgg cgtaatagca atcaggcgga
    20761 aaaaagagag tgaaaaagcg cgctggaata ccttcgacag cctgtgtctg gtattcagtg
    20821 tggcgacatt ttttgttgct cccgtgcaaa acctggcctg gggtggcgta tttaaactga
    20881 aagatactgg ctatccggta tttcgttttg ctaaggatgt catcgtcaat aataacgagg
    20941 tgattgaaga gcaagaacgg atggcaaaac tgtccggaat gaaagatacc tggacggtca
    21001 ctgccgttaa gccgaagtat cagacctatg tggtggtgat cggagaaagc gcgcgtcgcg
    21061 atgccctcgg tgcctttggc ggtcactggg acaatacccc gtttgccagc agcgttaacg
    21121 gtttgatatt tgctgactac attgccgcca gtggctccac gcagaaatcg cttggcttaa
    21181 cgctcaatcg cgttgtcgat ggcaaaccac agtttcagga taactttgtc accctggcaa
    21241 atcgcgcggg cttccagacc tggtggtttt ccaaccaggg ccaaatcggc gaatacgata
    21301 ccgccatcgc cagcatcgcc aaacgtgcag atgaagtgta cttcctgaaa gaaggtaatt
    21361 ttgaagcaga taaaaacacg aaagacgaag cgttactgga tatgaccgct caagtgctgg
    21421 cgcaagagca ctcgcaaccg cagctgattg ttttacatct gatgggctca catccgcagg
    21481 cctgcgaccg gacacaaggc aaatacgaaa cctttgtgca atcgaaagaa acgtcgtgct
    21541 atctctatac catgacgcaa acagacgatt tactgcgtaa gctgtacgat cagttacgca
    21601 acagcggcag tagcttctcg ctggtttact tttctgacca cggtctggcc tttaaagagc
    21661 gtggcaaaga cgtgcaatac cttgcccatg atgataagta tcagcaaaat ttccaggtgc
    21721 cttttatggt catttcgagc gacgataaag cgcatcgggt gattaaagcc cgccgctctg
    21781 ccaatgactt cttaggcttt ttctcccagt ggacgggaat taaagcgaag gaaataaata
    21841 tcaaataccc gtttatatct gagaagaaag ccgggccgat atacatcacc aacttccagt
    21901 tacagaaggt agattacaac catctcggaa ccgatatttt cgacccgaaa ccttaaaaac
    21961 aaaaatccgc cccgagaggc ggatttttta tatcaccgaa gtgattagaa gcggtaacca
    22021 acaccggcaa tccaggtgcc tacgtcaacg ctacgaatac ggctctgctc gtaagagaag
    22081 tccagagcaa cgttttccat cgggttgaac tgcagaccag caccgtagga gaaaccgtag
    22141 tcgctggtgt cgtgtttgta ggtcgggtat tcagtggtct ggaatttacc ataacccaca
    22201 cccactacac cgtagatgct tgcccagtcg ttaatgcggt aagccggacc agcagtgatg
    22261 ccgtagtact ggtttttgtt gtagtcacca gagcttgcag tacggctttt ctcggtgtaa
    22321 gtgaaagaac cgatcacacc cagcgggctg ttgtcttctt catagcggta tttcaggttg
    22381 aaaccgccca ttttgttcat ttggccctga gcgtcgctct gtgcgtaacc gccagttacg
    22441 gtagaagtcg cagctacgga agtacctgcg gtgaaagcca gaactgcggc cagtgctgaa
    22501 agacatgcaa tttttttcat aaccacctca aatgtgattc aaataagtcc taagttttaa
    22561 atatatcaaa aattaatggg aaactcttcg cgatttgtga tgtctaacgg gccatttcat
    22621 gtaacagaac gtttccatac accgctatcc atctaaattt aaatcacttt ttcagagagc
    22681 tgcataagta ttacgcatgt tttccctgtc attcatccag attattccta atcaccagac
    22741 taatgattcc atcaatcctg gcgcatttta gtcaaaacgg gggaaaattt tttcaacaaa
    22801 tgctcaacca gcattgggta tatccagtac actccacgct ttacttaggt ttagatattt
    22861 gtgggagaaa ggatgcctgg ttcattacgt aaaatgccgg tctggttacc aatagtcata
    22921 ttgctcgttg ccatggcgtc tattcagggt ggagcctcgt tagctaagtc actttttcct
    22981 ctggtgggcg caccgggtgt cactgcgctg cgtctggcat taggaacgct gatcctcatc
    23041 gcgttcttta agccatggcg actgcgcttt gccaaagagc agcggttacc gctgttgttt
    23101 tacggcgttt cgctgggggg gatgaattat cttttttatc tttctattca gacggtaccg
    23161 ctgggtattg cggtggcgct ggagttcacc ggaccactgg cggtggcgct gttctcttct
    23221 cgtcgcccgg tagatttcgt ctgggttgtt ctggcggttc ttggtctgtg gttcctgcta
    23281 ccgctggggc aagacgtttc ccatgtcgat ttaaccggct gtgcgctggc actgggagca
    23341 ggggcttgtt gggctattta cattttaagt gggcaacgcg caggagcgga acatggccct
    23401 gcgacggtgg caattggttc gttgattgca gcgttaattt tcgtgccaat tggagcgctt
    23461 caggctggtg aagcactctg gcactggtcg gttattccat tgggtctggc ggtcgctatt
    23521 ctctcgaccg ctctgcctta ttcgctggaa atgattgccc tcacccgttt gccaacgcgg
    23581 acatttggta cgctgatgag catggaaccg gcactggctg ccgtttccgg gatgattttc
    23641 ctcggagaaa cactgacacc catacagctg ctggcgctcg gcgcaattat cgccgcttca
    23701 atgggttcta cgctgacagt acgcaaagag agcaaaataa aagaattaga cattaattaa
    23761 atttacattt ctgcatggtt atgcataacc atgcagaatt tatcgctact tttcctctac
    23821 accgtcttta tatatcgaat tatgcaaaag catatttatt ccgaaaattc ctggcgagca
    23881 aataaataag aattgttctt attaatatat ctaactcatt gagtttttat tggttttgtt
    23941 ttttcacgct tgttaccact attagtgtga taggaacagc cagaatagcg gaacacatag
    24001 ctggtgctat acttagtctc gttaattact gggacataac atcaagagga tatgaaatta
    24061 tgagtaccgc taaattagtt aaatcaaaag cgaccaatct gctttatacc cgcaacgatg
    24121 tctctgatag cgagaaaaaa gcaacagtag agttgctgaa tcgccaggtt atccagttta
    24181 ttgatctttc tttgattacc aaacaagcgc actggaacat gcgcggcgct aacttcattg
    24241 ccgtacatga aatgctggat ggcttccgca ctgcactgat cgatcatctg gataccatgg
    24301 cagaacgtgc agtgcagctg ggcggtgtag ctctggggac cactcaagtt attaacagca
    24361 aaactccgct gaaaagttac ccgctggaca tccacaacgt tcaggatcac ctgaaagagc
    24421 tagctgaccg ttacgcaatc gtcgctaatg acgtacgcaa agcgattggc gaagcgaaag
    24481 atgacgacac cgcagatatc ctgaccgccg cgtctcgcga cctggataaa ttcctgtggt
    24541 ttatcgaatc taacatcgaa taaatccatc gctgatggtg cagaacttta gtacacgata
    24601 aaggcggctt cctgacagga ggccgttttg ttttgtagcc cgcctcaacg cacttattta
    24661 gtgcatccat ctgctatctt cagctgatta agtaaatttt ttgtatccac atcatcacac
    24721 aatcgttaca taaagattgt tttttcatca ggttttacgc taaataatca ctgtgttgag
    24781 tgcacaattt tagcgcacca gtttggtgcc ccagaatggt gcatcttcag ggtattgccc
    24841 tataaatcgt gcatcacgtt tttgccgcat ctcgaaaaat caatgagttg caaaactggc
    24901 acgatttttt catatatgtg aatgtcacgc aggggatcgt cccgtggata gaaaaaagga
    24961 aatgctatga agtctgtatt aaaagtttca ctggctgcac tgaccctggc ttttgcggtt
    25021 tcttctcatg ccgcggataa aaaattagtt gtcgcgacgg ataccgcctt cgttccgttt
    25081 gaatttaaac agggcgataa atacgtgggc tttgacgttg atctgtgggc tgccatcgct
    25141 aaagaactga agctggatta cgaactgaag ccgatggatt tcagtgggat cattccggca
    25201 ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta ccatcaccga cgagcgtaaa
    25261 aaagcgatcg atttctctga cggctactac aaaagcggcc tgttagtgat ggtgaaagcc
    25321 aacaataacg atgtgaaaag cgtgaaagat ctcgacggga aagtggttgc tgtgaagagc
    25381 ggcactggct ccgttgatta cgcgaaagca aacatcaaaa ctaaagatct gcgtcagttc
    25441 ccgaacatcg ataacgccta tatggaactg ggcaccaacc gcgcagacgc cgttctgcac
    25501 gatacgccaa acattctgta cttcatcaaa accgccggta acggtcagtt caaagcggta
    25561 ggtgactctc tggaagcaca gcaatacggt attgcgttcc cgaaaggtag cgacgagctg
    25621 cgtgataaag tcaacggcgc gttgaaaacc ctgcgcgaga acggaactta caacgaaatc
    25681 tacaaaaaat ggttcggtac tgaaccgaaa taataacgct acgcctgtaa ggcgcactgg
    25741 cagttccctc tcccctctgg ggagaggatt agggtgaggg gcgcaaaccc gctccggggc
    25801 cattaattac cctgaatttg attatttaca ccacggtaac aggaacaaca tatgcagttt
    25861 gactggagtg ccatctggcc tgccattccg cttctgattg aaggtgccaa aatgaccctg
    25921 tggatctcgg tcctcggtct ggctggcggc ctggtgatcg gattgctggc aggttttgca
    25981 cgcaccttcg gtggttggat agccaaccac gtcgcgctgg tctttattga agtgatccgc
    26041 ggcacaccta tcgtcgtcca ggtgatgttt atctatttcg ccctgccgat ggcatttaac
    26101 gacttacgca tcgacccatt tactgcggcg gtggtcacca tcatgatcaa ctccggcgca
    26161 tacattgcgg aaatcacgcg tggtgcggtg ctgtctattc acaaaggttt tcgtgaagca
    26221 ggactggcgc tcggtctttc acgttgggaa accattcgct acgtcatttt accgctggca
    26281 ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca tcagcattaa agacacctcg
    26341 ctgtttattg ttatcggcgt ggcggaactg acccgtcagg ggcaagaaat tattgccggt
    26401 aacttccgcg cccttgagat ctggagcgcc gtggcggtgt tctatctgat tattaccctg
    26461 gtgctgagct ttattctgcg tcgtctggaa agaaggatga aaatcctgtg attgaattta
    26521 aaaacgtctc caagcatttt ggtccaaccc aggtgctgca caatatcgat ttgaacattg
    26581 ctcagggcga agtcgtggtg attatcgggc cgtccggttc cggtaaatcg accctgctgc
    26641 gctgcatcaa caaactggaa gaaatcactt ccggcgatct gattgtcgat ggcctgaagg
    26701 ttaacgatcc gaaagttgac gagcgcctga ttcgccagga agcaggtatg gtgttccagc
    26761 agttttacct cttcccgcat ctgacggcgc tggaaaacgt catgtttggt ccgctacgcg
    26821 tgcgtggcgc gaacaaagaa gaggcggaaa aactggcgcg tgagctgctg gcgaaagtcg
    26881 gtctggcaga acgtgcacat cactaccctt ccgaactttc tggtggtcaa cagcagcgtg
    26941 tggcgatcgc acgcgcgctg gcggtgaagc cgaaaatgat gctgtttgat gaaccgactt
    27001 ccgctcttga cccggaactg cgccatgaag tgctgaaggt tatgcaggat ctggctgaag
    27061 aagggatgac gatggtgatc gtgacacacg aaatcggttt tgccgagaaa gtagcttcgc
    27121 gcctgatctt tatcgacaaa ggccggattg cggaagatgg cgatccgcag gtgttgatca
    27181 agaacccacc gagccagcgc ttgcaggaat ttttgcagca cgtctcttaa cttctcagcc
    27241 tccctttcct ccgaaaggga ggcacaccag attcctctca tttaaaattg cccctcctcc
    27301 agcatctata cttatctttt tgctctgttt tctcactgga ggagtcatgc ggtggatcct
    27361 gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg gtatccatac ccggcgttac
    27421 aaccacaacg acaacggact caacgactga accggccccg gaaccggata tcgaacaaaa
    27481 aaaagcggcc tatggtgcac tggcggatgt gctggataat gacacctcac gtaaagagtt
    27541 gatcgaccag ttgcgcaccg tcgccgcgac gccccctgcg gaacccgtac cgaagatcgt
    27601 gccgccgacg ctggttgaag agcaaaccgt gctgcaaaaa gtcaccgaag tcagccgcca
    27661 ttatggtgaa gccctttccg cccgcttcgg acaactttat cgcaatatca ccggctcccc
    27721 gcataagccg tttaatccac aaaccttcag caatgcgttg acccattttt caatgttagc
    27781 ggtattagtg tttggttttt actggctgat tcgcctgtgc gcacttccgc tgtatcgcaa
    27841 aatgggccag tgggcgcggc aaaaaaatcg tgagcgcagt aactggttgc agcttccggc
    27901 gatgattatc ggggcgttta ttatcgacct gctgttactg gcactgacat tatttgtcgg
    27961 tcaggtttta agcgacaacc tgaatgcggg cagtcgcacc atcgctttcc aacaaagttt
    28021 gtttctcaac gcctttgccc tgattgaatt tttcaaagct gtactacgcc tgattttttg
    28081 cccaaatgtg gcggagctgc gcccgttcac gattcatgac gagaccgccc gttactggag
    28141 ccgtcgcctg agctggttaa gcagtttgat aggttatggc ctgattgtgg ccgtgccgat
    28201 tatctctaat caggtgaatg tacaaattgg cgcgctggcg aacgtcatca ttatgctgtg
    28261 catgaccatc tgggcgttgt acctgatctt tcgtaataaa aaagagatta cccaacattt
    28321 gctcaacttt gcggagcatt cgctggcctt tttcagcttg tttatccgcg cctttgcgct
    28381 ggtgtggcac tggctggcaa gcgcctattt tatcgtgctg tttttctttt cgttgttcga
    28441 tccgggcaac agcctgaaat ttatgatggg tgcaacggtg cgcagcctgg cgattattgg
    28501 tatcgcagca tttgtttccg gtatgttttc ccgctggctg gcgaaaacca tcaccctctc
    28561 gccacatact cagcgaaatt atccggagct gcaaaaacgg ctgaatggct ggctttcggc
    28621 ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggcg gtaatgctgc tgttgagcgc
    28681 atggggatta ttcgatttct ggaactggct gcaaaacggt gcggggcaga aaaccgtaga
    28741 tatcctgatc cgtatcgcac tcattctttt cttctcggcg gttggctgga cggtgctcgc
    28801 cagtttgatc gaaaaccggc tggcttcgga tattcatggc cgcccactac ccagcgcccg
    28861 tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg gtgattatca gtaccatcac
    28921 catcatgatt gtgttgtcgg aaatcggcgt taatatcgcg ccattgctgg caggtgccgg
    28981 ggcattaggt ctggctatct cttttggttc gcaaacgctg gtgaaagata ttatcaccgg
    29041 ggtatttatt cagtttgaaa acggcatgaa cactggagat ttggtgacta tcgggccgtt
    29101 gaccggcact gtggaacgga tgtcgattcg ctccgtgggc gtgcgccagg ataccggggc
    29161 gtatcacatc attccgtggt cttcgataac cacctttgct aacttcgtcc gcggtattgg
    29221 ttcggtagtg gcaaactacg atgttgatcg ccatgaagat gctgataaag ccaatcaggc
    29281 actgaaagat gcggtagcag aattgatgga aaacgaagaa attcgcgggc tggttattgg
    29341 tgaaccgaac tttgccggga ttgtcggctt aagcaatacc gcgtttacac tgcgtgtttc
    29401 gttcaccacg ctgccactca aacagtggac ggtccgcttt gccctcgaca gccaggtgaa
    29461 aaaacatttc gacctggcgg gtgttcgcgc gccggtgcag acttatcagg tgctgccagc
    29521 tccgggcgcg gccccggctg aaccgttgcc gccgggggag ccaacgcttt aacgctggcg
    29581 attgacaaaa cggcggcgct gctcgtcgtt cataaaggtc caggctataa agcggctctg
    29641 cttttgcccc tgggccatct ctttttttac caccttcacc gcacctacgt ccgtcaaagc
    29701 acgatacaac ggcggtaagt tttcaccacg agataccagc gaggtaaacc acatcacctg
    29761 cttcgcgaag cctttgctct cttcaatcat ctttttgata aaagcgacct caccgccttc
    29821 acaccacaac tcctgttgct gaccaccaaa gttcagtgca tcgtctttgt tcagccccag
    29881 gttacggcgt ttacgttcac tacctgcccg tgccgcagcg gcggaatcgt ggaatggcgg
    29941 gttacacaag gtcgcatcgt attgctcgtt tttatggatg atgccgttaa aaatcgcccc
    30001 actctctttt tgccgacgca gacgaatggc gcggttaagc cccggattag cactgatgat
    30061 cgcctgcgca ctgcttaacg cctggctgct ggtttcacta ccggtaaaac gccagccata
    30121 ttcatgtacg ccaatcagcg gataaataca gttcgcacca acaccgatat ccagaatgct
    30181 ggcattagct ggaattgttc cgcttgcttc agccagtaaa tcggcaaggt gatgaatata
    30241 atccgcccgg cccggtactg gcgggcagag aaaaccgtcc gggatatccc agttcgctac
    30301 ggcgtaaaaa tgggccagca acgccttatt gagcgccttc accgccagcg gattggcaaa
    30361 gtctacgctt tgctccccgg cgggtgtaag cgtgaggaat tgcctgagtt caggattgac
    30421 ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg ttgcgcggat gcaaccccgg
    30481 tttctgggcg gacatagcat tctcctttaa gcagcggcgt aagatacccg cataacgcga
    30541 cagggtaaat atctggctgc gtttatcatt tctaaaaacc ttcacatggc tatggtttat
    30601 ggtaattcac cataaaaatc atcatcatgc atgtcattga tttatcttat tttgtaaaaa
    30661 tatattgttc aaaaaataag cgcatctctg tcaatttttt gcgtcatttt catcgcgcag
    30721 gcgacctaca cttatctggc actacccgtt gtatttatga ggatgtcatt atgaaaaagt
    30781 gcctcacact actgattgcc accgtcctga gcggaatctc tctcacggct tatgccgcgc
    30841 aaccgatgag taacctggac agtggtcaac tacggcccgc cggtaccgtt tcggcaactg
    30901 gcgcatcaaa cctaagcgat ctggaggata aactggcaga aaaagcgcgc gaacaaggcg
    30961 cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca gatgttcggt actgcaatca
    31021 tctacaaata accccaccaa cgtaactgca ttcgcctctg gatgcagttt tcgtccaatt
    31081 tgtaagcaaa aaacatcaaa tatgaacgca atgtaaataa atgtatttct ttttcgcgca
    31141 atgggtgata gaaaatcgcg ccaagtgata atgcttatca aaattattat cacttgcacg
    31201 gacgctatca cgggattaac agtggcatcg catccgcaga gatgctttct cgtgacagtg
    31261 aaaatttcac catataagaa aaagtcacct gcaaaatgga aaacaatcgc aatttccctg
    31321 ccagacaatt tcattcgctc acgttctttg ccggtctttg tattggtatt acgcctgtgg
    31381 cgcaggcact cgctgccgaa gggcaagcta acgcggatga cacgctggtt gtcgaagcat
    31441 caacgccttc gctttatgcg ccacaacaat ctgccgatcc gaaattctcg cgtccggtag
    31501 cggatactac ccgcacgatg acggtgattt ctgaacaagt gattaaagat cagggtgcaa
    31561 ccaaccttac cgacgcgctc aaaaacgtcc ccggcgtggg tgcgtttttt gcgggtgaga
    31621 acggtaactc caccactggc gacgccattt atatgcgcgg tgccgatacc tctaacagta
    31681 tttatatcga tggcattcgc gatatcggca gcgtctcgcg cgacaccttc aataccgagc
    31741 aggtcgaagt aattaaaggg ccgtccggca ccgactacgg gcgcagcgcg ccgacaggct
    31801 cgatcaatat gatcagcaaa cagccgcgca atgattccgg cattgacgcc tccgccagta
    31861 ttggcagcgc ctggttccgc cgcggcacgc tggacgtcaa tcaggtcatt ggtgatacca
    31921 ctgcggtgcg cctgaatgta atgggcgaaa aaacgcacga tgccggacgc gacaaagtca
    31981 aaaatgagcg ttacggcgtc gccccttctg tcgcttttgg ccttggtaca gcgaatcgtt
    32041 tgtatcttaa ttatctgcat gtcacccagc acaacacgcc agacggcggc attccgacta
    32101 tcggtttacc gggttattct gccccatctg cgggaacagc ggctctgaat cattccggaa
    32161 aagttgatac tcataacttt tacggcacgg attccgatta cgacgattcg accaccgaca
    32221 ccgctaccat gcgttttgag cacgacatca acgataacac caccattcgc aatactaccc
    32281 gttggtcgcg cgtgaagcag gattacctga tgacggcgat tatgggcggg gcgtcgaata
    32341 ttacccagcc caccagcgat gtgaatagct ggacctggtc acgcacggcg aatactaaag
    32401 atgtgagtaa taaaattctc accaaccaga ccaacctgac ctcgacgttc tataccggcg
    32461 ctatcggtca tgatgtcagt accggcgtgg aatttacccg tgaaacgcag actaactacg
    32521 gcgttaatcc ggtgacgtta cctgcggtaa atatttatca tcctgacagc agcattcatc
    32581 ccggcggcct gacgcgcaac ggcgcaaacg ccaatggtca gacggatacc ttcgcaattt
    32641 acgccttcga tacgctgcaa atcacccgtg attttgagct gaacggcggg atccgtctgg
    32701 ataattatca tactgaatat gacagtgcca ccgcctgcgg cggcagcgga cgcggtgcca
    32761 tcacctgccc tgctggcgtg gcaaaaggtt ctccggtcac caccgtcgat accgccaagt
    32821 cgggcaatct ggtgaactgg aaagccgggg cgctgtatca cctgacggaa aacggcaatg
    32881 tctatattaa ctatgccgtt tcccagcagc ctccgggcgg caacaacttc gcccttgcgc
    32941 agtctggcag cggtaacagt gccaaccgca ccgattttaa accgcaaaaa gccaacacca
    33001 gcgagattgg caccaaatgg caggttctgg ataaacgcct gttgctcacc gccgcgctgt
    33061 tccgtactga tatcaaaaat gaagttgagc aaaatgatga cggaacttac tcgcaatacg
    33121 gtaagaaacg cgtcgaaggc tatgagatat ccgtggccgg gaatatcact cccgcgtggc
    33181 aggtgattgg cggctatacc cagcaaaaag caaccatcaa aaacggcaaa gatgttgccc
    33241 aggatggttc ctcatcgctg ccgtataccc cggagcacgc ctttacctta tggagccagt
    33301 atcaggcaac cgatgatatt tccgttggtg cgggcgcacg ctatatcggc agtatgcata
    33361 aaggttctga cggcgcggtg ggaacgccag cgtttaccga aggttattgg gtcgccgacg
    33421 ctaaactggg gtatcgggtt aatcgcaatc tcgacttcca gctaaacgtc tacaacctgt
    33481 ttgataccga ttacgtcgcc tcaatcaaca agagcggcta ccgttatcac ccgggcgagc
    33541 caagaacctt cttgctcacg gccaatatgc atttctgatt gagatgtggg gcgcaggccc
    33601 cactttttgg agaacttgta tgatgtacca cattcccggc gtgctatcgc cacaggacgt
    33661 cgcccgtttt cgcgaacaac tggaacaagc cgaatgggtg gatggacgcg tcaccactgg
    33721 cgcacaaggt gcgcaagtta agaacaatca acaggtagac acccgcagca cgttatacgc
    33781 cgccctgcaa aatgaggtgc tgaacgcggt taaccaacat gctttgttct ttgccgcggc
    33841 cttgccgcgt accctttcca cgccgctgtt taatcgctat cagaacaatg aaacctatgg
    33901 ttttcatgtg gatggcgcag tacgcagtca cccacaaaac ggctggatgc gcactgacct
    33961 ttctgccacg ctgtttttaa gcgatccaca aagctacgat ggcggcgaac tggtcgttaa
    34021 tgacaccttc gggcaacatc gggtaaaact tccggcaggc gatctggtgt tgtatccctc
    34081 cagcagcctg cattgcgtga cacccgtaac ccgcggcgta cgagtggcat catttatgtg
    34141 gatccagtcg atgatccgcg atgataaaaa gcgcgccatg ctgtttgaac tggacaacaa
    34201 tattcagtcg ctgaaaagcc gctacggcga aagtgaagag atcctgtcgc tgctcaatct
    34261 ttatcataat ctgctgcggg aatggtcgga gatctgacgc ttgaactgcc cgagttcgtc
    34321 cctatattag ctacacttaa ctgtacaagt attgatatgg ggaggacgat atggcatccg
    34381 gttgggctaa cgatgacgcc gtcaacgaac agatcaacag tacaattgaa gatgcgattg
    34441 cccgcgctcg gggtgaaatt ccgcgtggcg aaagcctgta tgaatgtgaa gagtgcggag
    34501 cccccatccc gcaagcccgt cgggaagcca ttcctggtgt gcgcttatgc attcattgtc
    34561 agcaggagaa agatttacaa aaaccagctt atacaggata taatcgcaga ggttcgaaag
    34621 acagccagtt acgttaacta caactggcgg gatgtatagc gagtataatt tgtattttgc
    34681 gtggcattcc gtgcctttaa cgctgtcgtg actgccacgc tgcataaaac aagtccataa
    34741 gttcaacaaa taaatggatt atcagtatgt tagatatcaa tcaatttatt tgaacaaggc
    34801 ggtcaattct cttcgatttt atctctcgaa aaaaaacgtg atactcatca catcgacgaa
    34861 acaacgtcac ttatacaaaa atcacctgcg agagattaat tatgaaaact atcaatactg
    34921 ttgttgctgc tatggctctt tcaactctgt catttggcgt gtttgccgcg gaaccggtaa
    34981 cggcatccca ggcacagaac atgaataaaa tcggcgtggt ttctgccgat ggcgcatcca
    35041 ccctcgatgc cctggaagcg aaactggctg agaaagctgc ggcagctggg gcgagtggat
    35101 acagcatcac ttctgccacc aacaacaata aattaagcgg tactgcggta atttataagt
    35161 aattcgtaga acggataagg cgtttacgct acgtccagca caagtatttc cctgtctgtt
    35221 tgccgacaga cgcatatgct ctaaccctca ttgatcctat gttacccttg tttgcccgtc
    35281 cgccactgga cgggcttttt tttagctggc taactgctga cagaaagcct gcaacgtctc
    35341 ttccggcata ccaacctttc gcgccgcatc acaaatggcc tgccagcttc ccgcatccaa
    35401 tggaatcccc tgctcctgcc gttcgcgacg cgtgttaact tcccactcgc ccggtagcaa
    35461 aatcggctta tcgtcatcat gcggcgaggc tttcacccac tcggcaaagg cttcggtctg
    35521 cgcgctacaa tccggcgcgc cgaacagttc cgggttgatg atgatagtgg tcatgcagtt
    35581 aagaatggca tcgggactgg tttgtaacgt tttctgatgc gtcgttttac cgccggaaag
    35641 cgccccacca agaatttcac acatcgctgc aagggcgtag cctttatgtt cggcaaaggt
    35701 caacagcgaa cccaacggcg actcctgcat taccgccgga ttggtcgtcg gcatgccgtt
    35761 aacgtcaatc aggcaacctg gcggcacggg gacgccttta tgccaggcga cgcgggtttt
    35821 gccaaatgcg atggcgctgg tggcgtaatc aagcaacagc gggaaattat ctttacgagg
    35881 gaaaaccaca cagaacggat tggtgccaaa gcggctgtcg cgaccgtgga acggcgcgac
    35941 cattggaata ccgaccacgc taacaaagtg gatagagaca aaccccgccg ctgcacactg
    36001 ctccgcccag taaccgatac ggccgatatg atgcgagtta tgtagcgcca cggcagcaat
    36061 accgtgctga tgcgctttct caatccccag cgccatcgct tcgtgtgccg cgacctgacc
    36121 aaatgcgcga tcgccatcaa gcgtaaccac cgcgcccgct tctttaacga ttttggcatg
    36181 gtggttaatt tgcagatgcc cctgactcca ggaacgcaca tagcttggga tcatgccaat
    36241 accatgtgaa tcatgccctg ccaggtttgc cgcgattaaa tgatcggcaa ctaatttcgc
    36301 ttcttgttcc tcgctaccca tctgacgaaa tacagcctga ataaaactgt gcagcgtctg
    36361 agcatcaaag cgatgaccac tttccatggt ttactcctgt ttatgttatg tgtttgttgt
    36421 ttttttatgc tgcacgccgg gcgcgggaag gtcaataaag atgcaggaat ttcatattgc
    36481 ggtggggtaa aagccggatg acatggctca tccggctgag ataaaaatta aaatgcctga
    36541 ttaatgcgcg ccaggccgtc gttgatagtt gccgcttcac ccgtagccat caggcagcaa
    36601 gccatctgga ttttcagcga ttcaggaatc ggttcgctgc cagcaaggca acgcttaatc
    36661 cactgcgccg tggtttccgg atcttttgct tgtggcagta actcgctacc agcagtgtcc
    36721 tgtttttcat acagtacccg catcccttca cggtcaatga gattgatctg cgggcagcgc
    36781 tgcggattag catacacttc accttcagta ccatgcatta gtagcgcccg cccgccgata
    36841 tcactaaaga acttcgcgac gcgtccaatg tattccggat gcgaaacgct ggaaagacgc
    36901 agtgcctcac cttcggcaaa tggcgtcgcc agtttcgcca gggtatgtgc actgttacgc
    36961 acgcccatcc gccagcgcat cgccagttgt ttttccagcg gcgggcaaaa cgcgccgaca
    37021 ggcataaaca ccggttgatg ttcgtcgagc ttcgcctgcg cctgcccgcc gtgcaacgtt
    37081 ggcgtaatac ccatcagttc gaaaatggtt tctgtcagca cgcgggttgg atcttcgcta
    37141 accccgtgaa ccaccacagg aaaaccgagt ttgtgcagga gaatcgccaa caacggcgtc
    37201 aggttggcct gtttacgcgc gccgttgtaa ctggggatga caatcggcat cggcttgtct
    37261 gcaggcggcg tcagcttgat ggtgtgattt tgcatggctt cgtaaaagcc gagcatctct
    37321 gcttcccctt cccctttgat acgcagcgca atcaatacgc cgcccaactc aaggtcaggg
    37381 acttcaccat tgagcatatg ggcatacaga ccgcgcgcag tatcccggtc taaatcgcgc
    37441 gcgtggtttt tcccgcgccc gatctcttta atgattttgc gatagtccat ttacgactcc
    37501 ttacctgact cacatcatta acgccgccga cggcgtgggg atttcgtttt ttctttcttt
    37561 ttaactatac cttcagggac ttccggttgc tctatcggaa ataccggtaa tgcatccagt
    37621 agtcgcttgc cataattttt ggtcagcaag cgtttgtcgt agataaccac ttccccccag
    37681 caaccgtggc ttcgaatcag tcgcccaacc tgctgaatca ggttaaacga agcgctcggc
    37741 aggctttgca cctcaaacgg atagcggttg aggcttttca gccattcacc ttcggtgatc
    37801 accaccggac tgtcgatggg cggaaaagcg atcttgtgga tatgcacctg actgagcaga
    37861 tcgcctttca gatcaagacc ttcggcaaag gactgtaagc ccaccagcac gctacgctca
    37921 ccgttggcaa cgcgtttgcg gtgcagttca actaagcgat aacgtggctg atcgccctgc
    37981 accagcaaca tcaggcgtaa atccgtcaca tagtcgagaa agcgctgcat cgcacgcccg
    38041 ctggcaaaca gtaccaacat accgagatgt tttttgctct ccacctgctc acggaaaaag
    38101 gctgccattt cagcaatgtg ctgctcttcg ttgtcgatgg aaggctcaaa gcgcatccgg
    38161 ggaataacaa ttttgccctg ttcgcagtgg ttaaagggcg aatccagcgc cacaaagcgg
    38221 tcgcccgctt tctctttcag accactcatc tcctgtaaac gcgaaaaact gttcagcgaa
    38281 cgtaaggtcg cggaagtgac aataatgtgc ggaatactgc gccacagcag cctttccagc
    38341 tgatcgctga cacgaatacc cacacagtga aaccagagat gcagctgccc ttcgcgctct
    38401 tcccgcgtcg cccatttagt caccggcgca ccggaagatt gcgccagaga cgccagccgc
    38461 cagagtttgc tttgcgcctc gaacatcccc aacgcgcgat tcatctgcaa aatcaaccga
    38521 tgcagacgta caatgtcatg gctaccggtt ttctcactta aatcgttaag aaataactcc
    38581 gccaggccac gcagcatctc ggtgagtttt gccagccgct ggcagatctc cagcacttca
    38641 tcaggcagtt cgcccatcgc aaaacggtgc tctgcctcct gcccggcagg catgtagaga
    38701 ttgagaatgt tgtttaacga ggcgataagc tcatacagct cttcacaatg cgcattcaaa
    38761 cgttcaggga tcgccagcgg tgggatggtc ttcgggcgaa actgctccat gcaggtagcg
    38821 accagtttcg tgaacaagtc cagctgtagc cgataccagg gcgcggtgat ttcggcgctc
    38881 atctccagcg catcccgcgc cacatctggc aggtgatgac cttcgtccag caccagcagc
    38941 agatttttcg ggtcaggtag tacggcttcg ctttccatcg ccgccatcac cagcgcatgg
    39001 tttgccacca ccacttccgc ttcctgaatc tcccgacgag caacaaaaaa cgggcattca
    39061 cgatagtagt aacagttgcg gttgaggcag ctggctttgt cggtacttaa acgacgccag
    39121 agatcgtcat caatggcgat atccgtatga tcgcgcagac catcccattt ataagtgtcg
    39181 agatcgccct tcagcttcgc acaacgtttt tgctcttcct gattgttcgg cgtcagctcg
    39241 tcgtcaagaa acgccagcag atcctgttgc gtgggttcag tactggcaag cgctgtcaga
    39301 ttacgcggac aaacgtagcg cccacgccca aaagcagcag tgaatttaag atcgggaatg
    39361 atctttttca gcagaggcaa atctttactg tagatctgat cctgcaatgc cacgttggcg
    39421 gtactcacca ccagcgtttt ttgctcttcg cgggcaatgg cgatgccggg aatcaaatag
    39481 gagagcgttt tcccaacgcc ggtgggtgct tcaatcgcca gatgccgccc ttcttctccg
    39541 gccagcgttt tggcgacgtc cgcaatcatc tgccgctgcg gcgcacgggg aataaagtcg
    39601 gggatctgtt cctgaagcgc cttataccag gcggcaattt gcgctttaag cgcggcggtt
    39661 aatgccatga gaaaacctga aatactgtat aaacagccaa tattgtggca tttttcggtt
    39721 ttcaggggta atggtttttc tgtttctgga aagcggctcg caataaccag caggatgatg
    39781 cgtaaagcgc ttcgcgctaa attcgaacaa catgcagaac tgcgtacact cttgctggca
    39841 accgcgtccg caaaactggt tgagcatacg caaaatgatg cttactgggg agatagcggt
    39901 aatggtcagg gcaagaatcg cctggggtac cttttaatgg cgttgcgcgg acaattggct
    39961 gcggagaagt aacgcatcgg gtggcttgtg ggcgttaaaa cagagaaatc tttttgccgc
    40021 gctcgcaaat ggctgacaag tgcttgttgt gaaatgccgg atgcggcgta aacgccttat
    40081 ccggcctaca aaagcatgca aattcaatat attacagagt tcacgtaggc ctgataagcg
    40141 cagcgcatca ggcagttttg cgtttgttca tcagcctgat gccgggcatc gcccggcatc
    40201 aaaagattac tgcgcagcgg caggtttacg tggacggcgg cgacgttgtt gttcgcctgc
    40261 cggtttggca tcgccgaggc ggcgagacgg tttttctgca ggtttcgcac ttgcagattt
    40321 tgcgccacct tctccacggc gtggttgctg ttgaccgcga ccaccacctt gcccacgacc
    40381 gccgccacgt tgctggcgac cgttctggat cggttcggct ttgattgacg ggtccggctc
    40441 ataacccgga atcgcaatgc gcgggatctc ttttttcagc agtttttcga tatcacgcag
    40501 cagtttgtgt tcatcaacac acaccagcga caacgcttca ccggtagcag ccgcacgacc
    40561 ggtacgcccg atacggtgga cataatcttc cggtacgttt ggcagttcat agttgaccac
    40621 gtgcggcagc tcttcaatat ccaggccgcg cgcagcgatg tcagttgcca ccagtacacg
    40681 aatatcgccc gatttaaaat cagccagcgc acgagtacgc gcaccttgcg atttattacc
    40741 gtggatcgcc gcactacgga tgccatcttt attaagctgt tcagccagat ggttagcgcc
    40801 atgtttggta cgggtaaaca ccagcacctg ctgccagttc cctttcccaa tcatatgcga
    40861 cagcaattcg cgtttgcgtt tcttatcgac aaagtgaacg tgctgggtca cctgatcaga
    40921 cgcggtattg cggcgtgcca cttcgatttc cagcgggttg tgcaacagtt tttccgccag
    40981 ggctttaata tcgtcagaga aggtcgcgga gaacaacagg ttctggcgct tcgctggcag
    41041 ttttgttaac acgcgacgga tatcgtggat aaagcccatg tcgagcatgc ggtcagcttc
    41101 atcaaggacg aggatttcaa cctgatccag cttcactgca ttttgatgtt ccaggtccag
    41161 cagacgtccc ggcgttgcca ccagcacatc aacgccgccg cgcaacttca tcatttgtgg
    41221 gttaatgctg acaccgccaa acaccaccag cgaacgaatg ttcagatatt tgctgtaatc
    41281 acggacgttt tcgccaatct gcgccgccag ttcacgggtc ggggtaagaa tgagcgcgcg
    41341 taccggacga cgccctttgg cgtgcggctg gcgagtgatc aggtgttgca acagcggcag
    41401 cgtaaagccc gctgttttgc cggtaccggt ctgggcgctg gccatcaggt cgcggccttc
    41461 cagcaccgca gggatcgcct gctgctgaat aggggtgggt tcacggtaac cctgctcggc
    41521 aacggcgcgc aggatatcag ggcttaaacc caaagaatcg aaagacataa ctactccgaa
    41581 ccgccccgac cgttaccggt gtagttttca gggagatact gaaaaggaaa atgacaaaaa
    41641 ccacaatgtc atgagggagc ggagtgtagc agtttttgtg acgcagcgca taaattatcc
    41701 ctgtgactgg cgttgctaaa attcttagcc atactggaca actctcatat ggagtcataa
    41761 tcttaatcaa tcatttgatt aagaatggac tgtgcgatga ataatcctgc catgacaatc
    41821 aagggtgaac aggcgaagaa acagctgatt gctgccgcac tggcgcagtt tggtgaatat
    41881 ggaatgaacg ccaccacccg cgagatagcc gcccaggccg ggcagaatat cgctgccatc
    41941 acctattact tcggttcgaa agaggatttg tacctcgcct gcgcccagtg gattgccgat
    42001 tttattggcg agcagttccg tccacacgcc gaagaagccg aacgtttgtt cgcgcaacca
    42061 cagcctgatc gcgcagctat ccgtgaactg atccttcgcg cctgcaggaa catgattaag
    42121 ctgctcaccc aggatgacac cgtcaacctc agcaaattta tctcccgtga gcagctctct
    42181 cccacggcag cctatcacct ggtgcacgaa caggtgatta gtccgctaca cagccacctt
    42241 acacgcctga ttgccgcctg gaccggctgc gacgctagcg atacccgcat gatccttcat
    42301 acccatgcgt tgattggtga gattctggcg tttcgtcttg gcaaagaaac tattctgtta
    42361 cgtaccggct ggaccgcgtt cgatgaagaa aagaccgaac tgatcaacca gacggtgacc
    42421 tgtcatattg acctgatttt gcaaggatta tcgcaaagga gtttgtagtg atgaaaaaac
    42481 ctgtcgtgat cggactggcg gtagtggtac ttgccgccgt ggttgccgga ggctactggt
    42541 ggtatcaaag ccgccaggat aacggcctga cgctgtatgg caacgtggat attcgtacgg
    42601 tgaatcttag tttccgtgtt ggggggcgcg ttgagtcgct ggcggtggac gaaggtgatg
    42661 cgatcaaagc gggccaggta ctgggcgaac tggatcacaa gccgtatgag attgccctga
    42721 tgcaggcgaa agcgggcgtt tcggtggctc aggcgcagta tgacctgatg cttgccgggt
    42781 atcgagatga agaaatcgct caggccgccg cagcggtgaa acaggcgcaa gctgcctatg
    42841 actatgcgca gaacttctat aaccgccagc aaggactgtg gaaaagccgc accatttcgg
    42901 caaatgacct ggaaaatgcc cgctcctcgc gcgaccaggc gcaggccacg ctgaaatcag
    42961 cgcaggataa attgcgtcag taccgttccg gtaaccgcga acaggacatc gcccaggcga
    43021 aagccagcct cgaacaagcg caggcgcaac tggcgcaggc ggagctgaat ttacaggact
    43081 caacgttgat agccccgtct gatggcacgc tgttaacgcg cgcggtggag cctggcacgg
    43141 tgctcaatga aggtggcaca gtctttaccg tctcactgac acgtccggtg tgggtacgtg
    43201 cttatgttga tgaacgtaat ctcgaccagg cccagccggg gcgcaaagtg ctgctttata
    43261 ccgacggtcg cccggacaaa ccgtatcacg ggcagattgg tttcgtttcg cctaccgctg
    43321 aatttacccc gaaaactgtc gaaacgccgg atctgcgtac cgacctcgtc tatcgcctgc
    43381 gtattgtggt gaccgacgcc gatgatgcct tacgccaggg aatgccggtg acggtgcaat
    43441 tcggtaacga ggcaggacat gaatgatgcc gttattacgc tgaacggtct ggaaaaacgc
    43501 tttccgggca tggacaagcc cgccgtcgcg ccgctcgact gtaccattca tgctggttat
    43561 gtaacggggc tggtggggcc ggatggcgca ggtaaaacca cactgatgcg catgctggcg
    43621 ggattactga aacccgatag cggcagcgcc acggtgattg gctttgatcc gatcaaaaac
    43681 gacggcgcgc tgcacgccgt gctcggctat atgccgcaga aattcggcct gtatgaagat
    43741 ctgacggtga tggagaacct caatctgtac gcggatttgc gcagcgtcac cggtgaagcg
    43801 cggaagcaga cttttgctcg cctgctggaa tttacatctc ttggcccgtt taccggacgc
    43861 ctggcgggca agctctccgg cggaatgaag caaaaacttg gcctggcctg taccctggtg
    43921 ggcgaaccga aagtgttgct gctcgatgaa cccggcgtcg gcgttgaccc tatctcacgc
    43981 cgcgaactgt ggcagatggt gcatgagctg gcaggcgaag gaatgttaat tctctggagc
    44041 acctcgtatc tcgacgaagc cgaacagtgc cgtgacgtgt tactgatgaa cgaaggcgag
    44101 ctgctgtatc agggagaacc gacggccctg actcaaacta tggccggacg cagctttctg
    44161 atgaccagcc cgcacgaggg caaccgcaaa ctgttgcaac gggcattgaa actgccgcag
    44221 gtcagcgacg gcatgattca ggggaaatcg gtacgtctga tcctcaaaaa agagaccacg
    44281 ccagacgata ttcgccatgc cgacgggatg ccggaaatca acatcaacga aactacgccg
    44341 cgttttgaag atgcgtttat tgatttgctg ggcggtgccg gaacctcgga atcgccgctg
    44401 ggcgcaatat tacatacggt agaaggcaca cccggcgaga cggtgatcga agcgaaagag
    44461 ctgaccaaaa agtttggcga ttttgccgcc accgatcacg tcaactttgc cgtcaaacgc
    44521 ggggagattt ttggtttgct ggggccaaac ggcgcgggta aatcgaccac ctttaagatg
    44581 atgtgcggtt tgctggtgcc gacctctggt caggcgctgg tgctgggcat ggatctgaaa
    44641 gagagttccg gtaaagcgcg ccaacatctc ggctatatgg cgcaaaaatt ttcgctctat
    44701 ggcaacctga cggtcgaaca gaatttacgc tttttctctg gtgtgtatgg cttacgcggt
    44761 cgggcgcaga acgaaaaaat ctcccgtatg agcgaggcgt tcggcctgaa aagtatcgcc
    44821 tcccacgcga ccgatgaact gccattaggt tttaaacagc ggctggcgct ggcctgttca
    44881 ctgatgcatg aaccggacat tctgtttctc gacgaaccga cgtccggcgt tgatcccctc
    44941 acccgccgtg aattttggct acatatcaac agtatggtag agaaaggcgt cacggtgatg
    45001 gtcaccaccc actttatgga tgaagcggaa tattgcgacc gcatcggcct ggtgtaccgc
    45061 gggaaattaa tcgccagcgg cacaccggac gatttgaaag cgcagtcggc caacgatgag
    45121 caacccgatc ccacgatgga gcaagccttt attcagttga tccacgactg ggataaggag
    45181 catagcaatg agtaatccga tcctgtcctg gcgtcgcgtg cgggcgctgt gcgtcaaaga
    45241 gacgcggcag atcgttcgcg atccgagtag ctggctgatt gcggtagtga tcccactgct
    45301 actgctgttt atttttggat acggcattaa cctcgactcc agcaagctgc gggtcgggat
    45361 tttactggaa cagcgtagcg aagcggcgct ggatttcacc cacaccatga ccggttcgcc
    45421 ctacatcgac gccaccatca gcgataaccg tcaggaactg atcgccaaaa tgcaggcggg
    45481 gaaaattcgc ggtctggtgg ttattccggt ggatttcgcc gaacagatgg agcgcgccaa
    45541 cgccaccgca ccgattcagg tgatcaccga cggcagtgag ccgaataccg ctaactttgt
    45601 gcaggggtat gtcgaaggga tctggcagat ctggcaaatg cagcgagcgg aggataacgg
    45661 gcagactttt gaaccgctta ttgatgtaca aacccgctac tggtttaacc cggcggcaat
    45721 aagccagcac tttattatcc ccggtgcggt gaccattatc atgacggtca tcggcgcgat
    45781 tctcacctcg ctggtggtgg cgcgagaatg ggaacgcggc accatggagg cgttactctc
    45841 tacagagatt acccgcacgg aactgctgct gtgtaaactg atcccttatt actttctcgg
    45901 gatgctggcg atgttgctgt gtatgctggt gtcagtgttt attctcggcg tgccgtatcg
    45961 cggttcgctg ctgattctgt tttttatctc cagcctgttt ttactcagta ccctggggat
    46021 ggggctgctg atttccacga taacccgcaa ccagttcaat gccgctcagg tcgctctgaa
    46081 cgcggctttt ctgccgtcga ttatgctttc cggctttatt tttcaaatcg acagtatgcc
    46141 cgcggtgatc cgcgcggtga cgtacattat tcccgctcgt tatttcgtca gcaccctgca
    46201 aagcctgttc ctcgccggga atattccagt ggtgctggtg gtaaacgtgc tgtttttgat
    46261 cgcttcggcg gtgatgttta tcggcctgac gtggctgaaa accaaacgtc ggctggatta
    46321 gggagaagag catgtttcat cgcttatgga cgttaatccg caaagagttg cagtcgctgc
    46381 tgcgcgaacc gcaaacccgt gcgattctta ttttacccgt gctgattcag gtgatcctgt
    46441 tcccgttcgc cgccacgctg gaagtgacca acgccaccat cgccatctac gatgaagata
    46501 acggcgaaca ctcggtagaa cttactcaac gttttgcccg cgccagcgcc tttacccatg
    46561 tgctgctgtt gaaaagcccg caggagatcc gcccaaccat cgacacacaa aaggcattac
    46621 tcctggtgcg tttcccggct gacttctcgc gcaaactgga taccttccag accgcgccgt
    46681 tgcagttgat cctcgacggg cgtaactcca acagtgcgca aattgccgcc aactacctgc
    46741 aacagatcgt caaaaattat cagcaggagc tgctggaagg aaaaccgaaa cctaacaaca
    46801 gcgagctggt ggtacgcaac tggtataacc cgaatctcga ctacaaatgg tttgtggtgc
    46861 cgtcgctgat cgccatgatc accactatcg gcgtaatgat cgtcacttca ctttctgtcg
    46921 cccgcgaacg tgaacaaggt acgctcgatc agttactggt ctcaccgctc accacctggc
    46981 agatcttcat cggcaaggcc gtaccggcat taattgtcgc caccttccag gccaccattg
    47041 tgctggcgat tggtatctgg gcgtatcaaa tccccttcgc cggatcgctg gcgctgttct
    47101 actttacgat ggtgatttac ggtttatcgc tggtgggatt cggtctgttg atttcatcac
    47161 tctgttcaac acaacagcag gcgtttatcg gcgtgtttgt ctttatgatg cccgccattc
    47221 tcctttccgg ttacgtttcg ccggtggaaa acatgccagt atggctgcaa aacctgacgt
    47281 ggattaaccc tattcgccac tttacggaca ttaccaagca gatttatttg aaagatgcga
    47341 gtctggatat tgtgtggaat agtttgtggc cgctactggt gataacggcc acgacagggt
    47401 cagcggcgta cgcgatgttt agacgtaagg tgatgtaact tcttatcttt cgccagcaaa
    47461 gacactaccg ccgggccagc aaggattgcc agccctgcaa tcgccagcaa aaagttgttt
    47521 tccagtacgc gggacaacag ccacagcaca atcagcgctg cgccaaaata ccaggtcgtg
    47581 gtcagctctt ccagcacgtc tgccacctca cgccagcgtt gctcggggtg gcgctgaaac
    47641 accagcatca tcactaccgt gacacaatac agtgcgcata atcccacgcc gacgtgaacc
    47701 agagtcttac tcatccagcc caccaccagc agcgccacta ccagtaaatg caacataatc
    47761 tgccagcaac ttagacccgt tgcgacacga acacgttgtt gccacttcat ggcttctctc
    47821 ctgaagaatt tatctctact tactcagagt accgaacttt acggaaaatt ccgtaagacc
    47881 gcaccttttt gtgacgccga ctacactatt tcaatgagat aagaggcaaa gacaggaaaa
    47941 ttatgcccga tcaaacacaa caattttcgt tcaaggtgct caccatcaat attcacaaag
    48001 gctttaccgc gtttaaccga cgcttcattt tgccggaact tcgcgacgcc gtgcgtaccg
    48061 tcagcgccga tattgtttgc ctgcaggaag tgatgggcgc gcacgaagtt catccgctgc
    48121 atgtggaaaa ctggcccgat acctcgcact acgagtttct cgccgacacc atgtggagcg
    48181 atttcgccta cggtcgcaat gccgtatacc cggaagggca tcacggcaac gcggttcttt
    48241 cacgttatcc cattgaacat tatgagaatc gcgatgtttc ggtcgatggt gcggaaaagc
    48301 gcggcgtgct ctactgccgc attgtgccgc cgatgaccgg gaaagcgatt catgtgatgt
    48361 gcgtacatct gggcctgcgt gaggcgcacc gtcaggcgca gcttgcgatg ctcgccgaat
    48421 gggtgaatga gctgccggac ggcgaaccag tattggtggc gggtgatttc aacgactggc
    48481 ggcaaaaagc taatcatccg ttaaaaatgc aggccgggct ggatgagata tttacccgcg
    48541 cccacggacg cccggcgcgc acgtttccgg tgcaatttcc tctactacgt ctggacagga
    48601 tctatgtcaa aaatgccagc gccagcgcgc caaccgcgtt gccgctgcgg acatggcgac
    48661 acctttctga tcatgcccct ttaagtgcgg agattcattt atgaaatgta gctggcgcga
    48721 aggcaataag atccagttgc tggaaaacgg cgagcaatat tatcccgcga tgtttaaggc
    48781 gattggcgag gcgcaagaac gcatcattct tgaaacgttt atctggtttg aggatgacgt
    48841 cggcaaacag ctgcatgcgg cgctactggc agcagcgcaa cgcggggtga aagcggaagt
    48901 cttgctggat ggctacggtt cgccggatct cagcgatgag tttgtcaatg aactgacggc
    48961 agctggcgtg gtatttcgct actacgatcc ccgtcctcgc ctttttggta tgcgcaccaa
    49021 tgtgtttcgc cggatgcatc gcaaaattgt ggtgatcgac gcgcgtatcg cctttattgg
    49081 cgggctgaat tactccgccg agcatatgtc cagctacggt ccagaggcta aacaggatta
    49141 cgcggtacgc cttgaagggc cgattgtcga agatattctc cagtttgagc tggaaaacct
    49201 gcctggacag agcgcggcac gacgctggtg gcgacgtcat cacaaagcgg aagagaaccg
    49261 ccagccggga gaagcgcagg tattgctggt ctggcgcgat aacgaagaac atcgcgatga
    49321 tattgaacgt cactatctga aaatgctcac tcaggcgcgg cgagaagtca ttatcgccaa
    49381 cgcctacttc ttccccggct atcgattttt acacgccttg cgtaaagcgg cacggcgcgg
    49441 ggtgcggatc aaactgatca ttcagggcga accggatatg ccgattgtca gagtcggtgc
    49501 gcgcttgctg tataactatc tggttaaagg cggcgttcag gtatttgagt accgccgccg
    49561 cccgctccac ggcaaagtgg cattgatgga cgatcactgg gcgacagtag ggtccagtaa
    49621 tctcgatccg ctcagtttgt cactgaatct cgaagcaaat gtcatcatcc acgatcgtca
    49681 ttttaaccag acgctgcgcg ataatctgaa cggcattatt gccgcagatt gtcagcaggt
    49741 cgatgagacc atgctgccga aacgcacctg gtggaacctg accaaaagcg tgctggcgtt
    49801 ccacttttta cgccacttcc cggcgctggt tggctggctt ccggcacata caccacgtct
    49861 ggcgcaggtt gatccccccg cacaaccgac aatggaaacg caggatcggg tagaaactga
    49921 aaacacgggg gtaaaaccct gatgagtaaa tcacacccgc gctggcgctt agcaaagaag
    49981 atcctcacct ggctgttttt tatcgcggtg attgtgttgc tggtggtcta cgccaaaaaa
    50041 gtggactggg aagaggtctg gaaggtcatc cgcgactaca atcgcgttgc gctgctcagt
    50101 gcggtcgggc tggtggtcgt cagctatctg atttacggct gctatgacct gctcgcccgt
    50161 ttctactgcg gtcacaaact ggcgaagcgc caggtgatgc tggtgtcgtt tatctgctac
    50221 gccttcaacc tgacgctcag tacctgggtc ggcggcatag gtatgcgcta tcgtttgtac
    50281 tcgcggctag ggttaccggg cagcactatt acgcggattt tctcgctcag tattaccacc
    50341 aactggctgg gctatatttt gctggccggg attatcttta ccgcaggcgt ggtggagctg
    50401 ccggatcact ggtatgtcga tcaaaccacg ctgcgtattc tcggcattgg cttattgatg
    50461 attatcgcgg tttatttgtg gttttgcgct ttcgcgaagc accgccatat gaccatcaaa
    50521 gggcaaaagc tggtgctgcc ttcatggaaa ttcgccctcg cccagatgct gatttccagc
    50581 gttaactgga tggtcatggg agcgataatc tggctgttac ttggtcaaag cgtgaactat
    50641 ttctttgtac tgggcgtgtt gctggttagt agtattgccg gggtgattgt gcatattccg
    50701 gcggggattg gtgtgctgga agcggtgttt atcgcactac tggctgggga gcatacctcc
    50761 aaaggtagca ttatcgccgc cctactcgct taccgtgtgc tgtattactt tatcccactg
    50821 ctgctggcgc tgatctgtta cctgctactg gaaagccagg cgaagaagct gcgggcgaaa
    50881 aatgaagcgg cgatgtgaag ttcggttaag ttctttagta tgtgcattta cgactaatga
    50941 aaaaaccgcg tatgcctttg ccagacaagc gttatagcta tcgcggcaag tatcccaaag
    51001 aagccgataa aatagtaata gagtgttaat gcacctgtaa aagcgcaact aaccggactc
    51061 cacaactctt cgctatggca ttttcctgcc agttcaagga gcttatttcg tccatgcgag
    51121 ataatgcctg accataaaac aatcattaag caacttatta cccaaatggg ccgttcactc
    51181 attaatgcgg cattcctgat gatcaccagg gccagtcctg cggttgtcac caaacccaaa
    51241 gcggtaatga gccatatgcc gtttaatatc ctggcttcgt gctgaaaatc taatttattg
    51301 ctg
//
LOCUS       DAZGAP010000009        50051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGAP010000009 DAZGAP010000000
VERSION     DAZGAP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.009"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..1103
                     /gene="cyoA"
                     /locus_tag="ACHLAM_000451"
     CDS             156..1103
                     /gene="cyoA"
                     /locus_tag="ACHLAM_000451"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO7852040.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1125..3116
                     /gene="cyoB"
                     /locus_tag="ACHLAM_000452"
     CDS             1125..3116
                     /gene="cyoB"
                     /locus_tag="ACHLAM_000452"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO7852041.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3106..3720
                     /gene="cyoC"
                     /locus_tag="ACHLAM_000453"
     CDS             3106..3720
                     /gene="cyoC"
                     /locus_tag="ACHLAM_000453"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO7852042.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3720..4049
                     /gene="cyoD"
                     /locus_tag="ACHLAM_000454"
     CDS             3720..4049
                     /gene="cyoD"
                     /locus_tag="ACHLAM_000454"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO7852043.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4061..4951
                     /gene="cyoE"
                     /locus_tag="ACHLAM_000455"
     CDS             4061..4951
                     /gene="cyoE"
                     /locus_tag="ACHLAM_000455"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO7852044.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5100..6464
                     /gene="yajR"
                     /locus_tag="ACHLAM_000456"
     CDS             5100..6464
                     /gene="yajR"
                     /locus_tag="ACHLAM_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7852045.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILAATGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADTAANEALEVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6592..7083)
                     /gene="yajQ"
                     /locus_tag="ACHLAM_000457"
     CDS             complement(6592..7083)
                     /gene="yajQ"
                     /locus_tag="ACHLAM_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO7852046.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7251..8162
                     /gene="panE"
                     /locus_tag="ACHLAM_000458"
     CDS             7251..8162
                     /gene="panE"
                     /locus_tag="ACHLAM_000458"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO7852047.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGITVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8125..8715
                     /gene="yajL"
                     /locus_tag="ACHLAM_000459"
     CDS             8125..8715
                     /gene="yajL"
                     /locus_tag="ACHLAM_000459"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO7852048.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8769..10217)
                     /gene="thiI"
                     /locus_tag="ACHLAM_000460"
     CDS             complement(8769..10217)
                     /gene="thiI"
                     /locus_tag="ACHLAM_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFO7852049.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10423..10665
                     /gene="xseB"
                     /locus_tag="ACHLAM_000461"
     CDS             10423..10665
                     /gene="xseB"
                     /locus_tag="ACHLAM_000461"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO7852050.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10665..11564
                     /gene="ispA"
                     /locus_tag="ACHLAM_000462"
     CDS             10665..11564
                     /gene="ispA"
                     /locus_tag="ACHLAM_000462"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO7852051.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLEALERIHRHKTGALIRAAVRLGALSAGDKGRRALSVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            11589..13451
                     /gene="dxs"
                     /locus_tag="ACHLAM_000463"
     CDS             11589..13451
                     /gene="dxs"
                     /locus_tag="ACHLAM_000463"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO7852052.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEIVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13506..14480
                     /gene="yajO"
                     /locus_tag="ACHLAM_000464"
     CDS             13506..14480
                     /gene="yajO"
                     /locus_tag="ACHLAM_000464"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO7852053.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14534..15052)
                     /gene="pgpA"
                     /locus_tag="ACHLAM_000465"
     CDS             complement(14534..15052)
                     /gene="pgpA"
                     /locus_tag="ACHLAM_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO7852054.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15030..16007)
                     /gene="thiL"
                     /locus_tag="ACHLAM_000466"
     CDS             complement(15030..16007)
                     /gene="thiL"
                     /locus_tag="ACHLAM_000466"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO7852055.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16085..16504)
                     /gene="nusB"
                     /locus_tag="ACHLAM_000467"
     CDS             complement(16085..16504)
                     /gene="nusB"
                     /locus_tag="ACHLAM_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO7852056.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16524..16994)
                     /gene="ribE"
                     /locus_tag="ACHLAM_000468"
     CDS             complement(16524..16994)
                     /gene="ribE"
                     /locus_tag="ACHLAM_000468"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO7852057.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17083..18186)
                     /gene="ribD"
                     /locus_tag="ACHLAM_000469"
     CDS             complement(17083..18186)
                     /gene="ribD"
                     /locus_tag="ACHLAM_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO7852058.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18190..18639)
                     /gene="nrdR"
                     /locus_tag="ACHLAM_000470"
     CDS             complement(18190..18639)
                     /gene="nrdR"
                     /locus_tag="ACHLAM_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO7852059.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18790..19329
                     /gene="yajI"
                     /locus_tag="ACHLAM_000471"
     CDS             18790..19329
                     /gene="yajI"
                     /locus_tag="ACHLAM_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO7852060.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            19628..20512
                     /gene="tsx"
                     /locus_tag="ACHLAM_000472"
     CDS             19628..20512
                     /gene="tsx"
                     /locus_tag="ACHLAM_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO7852061.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20550..20897)
                     /gene="yajD"
                     /locus_tag="ACHLAM_000473"
     CDS             complement(20550..20897)
                     /gene="yajD"
                     /locus_tag="ACHLAM_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO7852062.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21027..21998)
                     /gene="secF"
                     /locus_tag="ACHLAM_000474"
     CDS             complement(21027..21998)
                     /gene="secF"
                     /locus_tag="ACHLAM_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO7852063.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22009..23856)
                     /gene="secD"
                     /locus_tag="ACHLAM_000475"
     CDS             complement(22009..23856)
                     /gene="secD"
                     /locus_tag="ACHLAM_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO7852064.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(23884..24216)
                     /gene="yajC"
                     /locus_tag="ACHLAM_000476"
     CDS             complement(23884..24216)
                     /gene="yajC"
                     /locus_tag="ACHLAM_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO7852065.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24239..25366)
                     /gene="tgt"
                     /locus_tag="ACHLAM_000477"
     CDS             complement(24239..25366)
                     /gene="tgt"
                     /locus_tag="ACHLAM_000477"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO7852066.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25421..26491)
                     /gene="queA"
                     /locus_tag="ACHLAM_000478"
     CDS             complement(25421..26491)
                     /gene="queA"
                     /locus_tag="ACHLAM_000478"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO7852067.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDERINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26584..27165
                     /gene="acpH"
                     /locus_tag="ACHLAM_000479"
     CDS             26584..27165
                     /gene="acpH"
                     /locus_tag="ACHLAM_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO7852068.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETCFWQFYPRMMEQASRKAL"
     gene            complement(27170..28987)
                     /gene="malZ"
                     /locus_tag="ACHLAM_000480"
     CDS             complement(27170..28987)
                     /gene="malZ"
                     /locus_tag="ACHLAM_000480"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO7852069.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSGGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29143..30516)
                     /gene="proY"
                     /locus_tag="ACHLAM_000481"
     CDS             complement(29143..30516)
                     /gene="proY"
                     /locus_tag="ACHLAM_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO7852070.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     SLKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            complement(30592..31911)
                     /gene="brnQ"
                     /locus_tag="ACHLAM_000482"
     CDS             complement(30592..31911)
                     /gene="brnQ"
                     /locus_tag="ACHLAM_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO7852071.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32318..33613)
                     /gene="phoR"
                     /locus_tag="ACHLAM_000483"
     CDS             complement(32318..33613)
                     /gene="phoR"
                     /locus_tag="ACHLAM_000483"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO7852072.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKVTRFSFVIPERLIAKNSD"
     gene            complement(33671..34360)
                     /gene="phoB"
                     /locus_tag="ACHLAM_000484"
     CDS             complement(33671..34360)
                     /gene="phoB"
                     /locus_tag="ACHLAM_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO7852073.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34550..35752
                     /gene="sbcD"
                     /locus_tag="ACHLAM_000485"
     CDS             34550..35752
                     /gene="sbcD"
                     /locus_tag="ACHLAM_000485"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO7852074.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            35749..38892
                     /gene="sbcC"
                     /locus_tag="ACHLAM_000486"
     CDS             35749..38892
                     /gene="sbcC"
                     /locus_tag="ACHLAM_000486"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO7852075.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIKEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSVREHLRQWQQQLTHA
                     EQKLNALTAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTMEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            39018..40214
                     /gene="araJ"
                     /locus_tag="ACHLAM_000487"
     CDS             39018..40214
                     /gene="araJ"
                     /locus_tag="ACHLAM_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO7852076.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGMVLGNVLSGRISGRYSPLRIAAVTD
                     FIIVLALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQ
                     IAFNLGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPV
                     LAKPLG"
     gene            complement(40353..41261)
                     /gene="mak"
                     /locus_tag="ACHLAM_000488"
     CDS             complement(40353..41261)
                     /gene="mak"
                     /locus_tag="ACHLAM_000488"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO7852077.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            41386..42297
                     /gene="rdgC"
                     /locus_tag="ACHLAM_000489"
     CDS             41386..42297
                     /gene="rdgC"
                     /locus_tag="ACHLAM_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO7852078.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42378..42458)
                     /locus_tag="ACHLAM_000490"
     CDS             complement(42378..42458)
                     /locus_tag="ACHLAM_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852079.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(42455..42784)
                     /locus_tag="ACHLAM_000491"
                     /pseudo
     CDS             complement(42455..42784)
                     /locus_tag="ACHLAM_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(42941..43225)
                     /gene="ppnP"
                     /locus_tag="ACHLAM_000492"
     CDS             complement(42941..43225)
                     /gene="ppnP"
                     /locus_tag="ACHLAM_000492"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO7852080.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43297..43974)
                     /gene="aroM"
                     /locus_tag="ACHLAM_000493"
     CDS             complement(43297..43974)
                     /gene="aroM"
                     /locus_tag="ACHLAM_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO7852081.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(44232..44423)
                     /gene="yaiA"
                     /locus_tag="ACHLAM_000494"
     CDS             complement(44232..44423)
                     /gene="yaiA"
                     /locus_tag="ACHLAM_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO7852082.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(44473..44997)
                     /gene="aroL"
                     /locus_tag="ACHLAM_000495"
     CDS             complement(44473..44997)
                     /gene="aroL"
                     /locus_tag="ACHLAM_000495"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO7852083.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45180..45638)
                     /gene="yaiI"
                     /locus_tag="ACHLAM_000496"
     CDS             complement(45180..45638)
                     /gene="yaiI"
                     /locus_tag="ACHLAM_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO7852084.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            45758..46567
                     /gene="proC"
                     /locus_tag="ACHLAM_000497"
     CDS             45758..46567
                     /gene="proC"
                     /locus_tag="ACHLAM_000497"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO7852085.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(46584..47699)
                     /gene="adrA"
                     /locus_tag="ACHLAM_000498"
                     /gene_synonym="dgcC"
     CDS             complement(46584..47699)
                     /gene="adrA"
                     /locus_tag="ACHLAM_000498"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO7852086.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(47801..48121)
                     /gene="psiF"
                     /locus_tag="ACHLAM_000499"
     CDS             complement(47801..48121)
                     /gene="psiF"
                     /locus_tag="ACHLAM_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO7852087.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(48240..49655)
                     /gene="phoA"
                     /locus_tag="ACHLAM_000500"
     CDS             complement(48240..49655)
                     /gene="phoA"
                     /locus_tag="ACHLAM_000500"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO7852088.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVVHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(49755..50015)
                     /gene="iraP"
                     /locus_tag="ACHLAM_000501"
     CDS             complement(49755..50015)
                     /gene="iraP"
                     /locus_tag="ACHLAM_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO7852089.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
BASE COUNT        12153 a        13373 c        12685 g        11840 t
ORIGIN      
        1 ttcttaaaat caacctgata tgttttgcaa catatgtgac ctgtaagcca aatccaagta
       61 acaggaattt aatcatgttt acagtaatgt aaccttcccg taaaatgccc acacacttta
      121 aacgccacca gatcccgtgg aattgaggtc gttaaatgag actcaggaaa tacaataaaa
      181 gtttgggatg gttgtcatta tttgcaggca ctgtattgct cagtggctgt aattctgcgc
      241 tgttagatcc caaaggacag attggtctgg agcaacgttc actgatactg acggcatttg
      301 gcctgatgtt gattgtcgtt attcccgcaa tcttgatggc tgttggtttc gcctggaagt
      361 accgtgcgag caataaagat gctaagtaca gcccgaactg gtcacactcc aataaagtgg
      421 aagctgtggt ctggacggta cctatcttaa tcatcatctt ccttgcggta ctgacctgga
      481 aaaccactca cgctcttgag cctagcaagc cgctggcaca cgacgagaag cccattacca
      541 tcgaagtggt ttccatggac tggaaatggt tcttcatcta cccggaacag ggcattgcaa
      601 ccgtgaatga aatcgctttc ccggcgaaca ctccggtgta cttcaaagtg acctccaact
      661 ccgtgatgaa ctccttcttc attccgcgtc tgggtagcca gatttatgcc atggccggta
      721 tgcagactcg cctgcatctg atcgccaacg aacccggtac ttatgacggt atctccgcca
      781 gctacagcgg cccgggcttc tcaggcatga agttcaaagc tattgcaaca ccggatcgcg
      841 ccgcattcga ccaatgggtc gcaaaagcga aacagtcgcc gaacagcatg tctgacatgg
      901 cagcgttcga aaaactggcc gcgcctagcg aatacaacca ggtggaatat ttctccaacg
      961 tgaaaccaga cttgtttgcc gatgtgatta acaagtttat ggctcacggt aagagcatgg
     1021 acatgaccca gccagaaggt gagcacagcg cacatgaagg tatggaaggc atggacatga
     1081 gccacgcgga atccgcccat taaaggggtt gaggaagaat aaagatgttc ggaaaattat
     1141 cacttgatgc agtcccgttc catgaaccta tcgtcatggt tacgatcgct ggcattattt
     1201 tgggaggtct ggcgctcgtt ggcctgatca cttacttcgg taagtggacc tacctgtgga
     1261 aagagtggct gacctccgtc gaccataaac gcctcggtat catgtatatc atcgtggcga
     1321 ttgtgatgtt gctgcgtggt tttgctgacg ccattatgat gcgtagccag caggctcttg
     1381 cctcggcggg cgaagcgggc ttcctgccac ctcaccacta cgatcagatc ttcaccgcgc
     1441 acggcgtgat tatgatcttc ttcgtggcga tgcctttcgt tatcggtctg atgaacctgg
     1501 tggttccgct gcagatcggc gcgcgtgacg ttgcgttccc gttcctcaac aacctaagct
     1561 tctggtttac cgttgttggt gtgattctgg ttaacgtttc tctcggcgtg ggcgaatttg
     1621 cgcagaccgg ctggctggcc tatccaccgc tatcgggaat agagtacagt ccgggagtcg
     1681 gtgtcgatta ctggatatgg agtctccagc tatccggtat aggtacaacg cttaccggta
     1741 tcaacttctt cgttaccatt ctgaagatgc gcgcaccggg catgaccatg ttcaagatgc
     1801 cagtatttac ctgggcatca ctgtgcgcga acgtactgat tattgcttcc ttcccaattc
     1861 tgacggttac cgttgcgttg ttgaccctgg atcgctatct gggcacccat ttctttacca
     1921 acgatatggg tggcaacatg atgatgtaca tcaacctgat ttgggcctgg ggccacccgg
     1981 aagtttacat cctgatcctg cctgttttcg gtgtgttctc cgaaattgcg gcaaccttct
     2041 cgcgtaaacg tctgtttggt tatacctcgc tggtatgggc aaccgtctgt atcaccgtgc
     2101 tgtcgttcat cgtctggctg caccacttct ttacgatggg tgcgggcgcg aacgtaaacg
     2161 ccttctttgg tatcaccact atgattatcg ccatcccgac cggggtgaag atcttcaact
     2221 ggctgttcac catgtatcag ggccgcatcg tgttccattc tgcgatgctg tggaccatcg
     2281 gttttatcgt caccttctcg gtaggcggta tgactggcgt gctgctggca gtaccgggcg
     2341 cggacttcgt tctgcataac agcctgttcc tgattgcgca cttccataac gtgatcatcg
     2401 gcggcgtggt cttcggctgc ttcgcaggga tgacctactg gtggcctaaa gcgttcggtt
     2461 tcaaactgaa cgaaacctgg ggtaaacgcg cgttctggtt ctggatcatc ggcttcttcg
     2521 ttgcctttat gccgctgtat gcgctgggct tcatgggcat gacccgtcgt ttgagccagc
     2581 agattgaccc gcagttccac accatgctga tgattgcagc cagcggtgca gtactgattg
     2641 cgctgggtat tctctgcctc gttattcaga tgtacgtttc tattcgtgac cgcgaccaga
     2701 accgtgacct gactggcgac ccgtggggtg gccgtacgct ggagtgggca acctcttccc
     2761 cgcctccgtt ctataacttt gccgttgtgc cgcacgttca cgagcgtgat gcattctggg
     2821 aaatgaaaga gaaaggcgaa gcgtacaaaa agcctgacca ctatgaagaa attcatatgc
     2881 cgaaaaacag cggtgcaggt atcgtcattg cggctttctc caccatcttc ggtttcgcca
     2941 tgatctggca tatctggtgg ctggcgattg ttggcttcgc aggcatgatc atcacctgga
     3001 tcgtgaaaag cttcgacgag gacgtggatt actacgtgcc ggtggcagaa atcgaaaaac
     3061 tggaaaacca gcatttcgat gagattacta aggcagggct gaaaaatggc aactgatact
     3121 ttgacgcacg cgactgccca cgcgcacgaa cacgggcacc acgatgcagg cggaaccaaa
     3181 gtcttcggat tctggatcta cctgatgagc gactgcattc tgttctctat cctgtttgct
     3241 acctatgccg ttctggtgaa cggcaccgca ggcggcccga caggtaagga cattttcgaa
     3301 ctgccgttcg ttctggttga aactttcctg ctgttgttca gctccatcac ctacggcatg
     3361 gcggctatcg ccatgtacaa aaacaacaaa agccaggttg tctcctggct ggcgttgacc
     3421 tggttgtttg gcgccggatt tatcgggatg gagctctatg aattccatca tctgattgtt
     3481 aacggcatgg gtccggatcg cagcggcttc ctgtcagcgt tctttgcgct ggtcggcacg
     3541 cacggtctgc acgtcacttc cggtcttatc tggatggcgg tgctgatggt gcaaatcgcc
     3601 cgtcgcggcc tgaccagcac taatcgtacc cgcatcatgt gcctgagcct gttctggcac
     3661 ttcctggatg tggtttggat ctgtgtattc actgttgttt atctgatggg ggcgatgtaa
     3721 tgagtcattc taccgatcac agcggcgcgt cccatggcag cgtaaaaacc tacatgacag
     3781 gctttatcct gtcgatcatt ctgacggtga ttccgttctg gatggtgatg acaggagctg
     3841 cctctccggc cgtaattctg ggaacaatcc tggcaatggc agtggtacag gttctggtgc
     3901 atctggtgtg cttcctgcac atgaatacca aatcagatga aggctggaac atgacggcgt
     3961 ttgtcttcac cgtgctaatc atcgctatcc tggttgtagg ctccatctgg attatgtgga
     4021 acctcaacta caacatgatg atgcactaag agcggcggtt atgatgttta agcaatacct
     4081 gcaagtaacg aaaccaggca tcatctttgg caacctgatc tcggtgattg ggggattcct
     4141 gttggcctca aagggcagca ttgattatcc cctgtttatc tacacgctgg ttggggtgtc
     4201 actggttgtg gcgtcgggtt gtgtgtttaa caactacatc gacagggata tcgacagaaa
     4261 gatggaaagg acgaagaatc gggtgctggt taaaggcctg atctcacctg ctgtctcgct
     4321 ggtgtacgcc accttgctgg gtattgctgg ctttatgctg ctgtggtttg gcgcgaatcc
     4381 gctggcctgc tggctggggg tgatgggctt tgtggtttat gtcggcgttt atagcctgta
     4441 catgaaacgc cactcagtct acggcacatt gattggttcg ctctccggcg ctgcgccgcc
     4501 ggtgatcggc tactgtgcag taaccggcga gttcgatagt ggcgcagcga tcctgcttgc
     4561 tatcttcagt ctgtggcaga tgcctcactc ctatgccatc gccattttcc gctttaagga
     4621 ttaccaggcg gcaaacattc cggtattgcc agtggtaaaa ggcatttcgg tggcgaagaa
     4681 tcacatcacg ctgtatatca tcgcctttgc cgttgccacg ctgatgctct ctcttggcgg
     4741 ttacgctggg tataaatatc tggtagtcgc cgcggcggtg agcgtctggt ggttaggcat
     4801 ggctctgcgc ggttataaag ttgctgatga cagaatctgg gcgcgcaagc tgttcggctt
     4861 ctctatcatc gccatcactg ccctctcggt gatgatgtcc gttgatttta tggtgccgga
     4921 ctcgcatacg ctgctggctg ctgtgtggta acacaacctc tcagttaaaa aggtgctacg
     4981 gcaccttttt tcttagcatt agaatcatat ccctctcgaa atatttacta aaaaatccgc
     5041 atgtttaccc cattcgtttg ccgctttaca ctagtcgcga atttaaaaca gaggtggtaa
     5101 tgaacgatta taaaatgacg ccaggtgagc gacgcgcgac ctggggttta gggaccgtat
     5161 tctcgttgcg catgctgggc atgttcatgg ttctgccggt tctgaccacg tacggcatgg
     5221 ctctgcaagg tgccagcgaa gcattaatcg gtattgccat tggtatttat ggtctgactc
     5281 aggccgtttt tcagattccg tttggcctgc tttccgaccg cattggtcgc aaaccattaa
     5341 ttgtcggtgg gctggcagtg tttgccgccg gtagcgttat cgctgcgctc tctgactcca
     5401 tctggggaat tattctgggc cgggcgctac aaggctccgg tgcgattgcc gctgccgtta
     5461 tggcgctgct ttccgatctc acgcgcgaac aaaaccgcac caaagcgatg gcgtttatcg
     5521 gcgtgagctt tggcattacc tttgccattg cgatggtgct tggcccgatc atcactcaca
     5581 aacttgggct gcacgcgctg ttctggatga tcgctattct ggcagcaacc ggcattgcgt
     5641 tgaccatttg ggttgtgccc aacagtagca ctcacgtact taatcgtgag tccggaatgg
     5701 tgaaaggaag tttcagtaaa gtgctggcgg aaccgcggct gctgaaactc aactttggca
     5761 ttatgtgtct gcatattttg ctgatgtcga cctttgttgc cctgcccgga cagatggctg
     5821 atgcggggtt cccggcggct gaacactgga aggtctacct ggcaacgatg ttaatcgcct
     5881 ttggctcggt cgtgcctttc attatctacg ctgaagttaa gcgcaaaatg aagcaagtct
     5941 ttgtcttttg cgtcgggctg atcgtggttg cggaaattgt gttgtggaac gcacaaacgc
     6001 agttctggca actggtggtc ggcgtgcagc ttttctttgt ggcgtttaat ttgatggaag
     6061 cccttctgcc ttcacttatc agtaaagaat cgccagcagg ttacaaaggt acggcgatgg
     6121 gtgtttactc caccagccag tttcttggcg tggcgattgg cggttcgctg ggcggctgga
     6181 ttgacggcat gtttgacggt cagggagtat ttctcgctgg cgcaatgctg gccgcagtgt
     6241 ggctggcagt cgccagtacc atgaaagaac cgccgtatgt cagcagcttg cgcattgaaa
     6301 tcccggcgga cactgccgca aacgaagcgt tagaagtacg tttgctggaa actgaaggcg
     6361 tcaaagaagt gttgattgca gaagaagaac attcagcgta tgtgaaaatc gacagcaaag
     6421 tgacgaatcg ctttgaggtt gaacaggcaa ttcgccaggc ataaaagcga ggcgaaattg
     6481 cctgctgcgc tacgcttatc aggcctacta agttcctgag ttacaaacct ttgtaagcca
     6541 aatcaggcgt tcatgccgca tctgacatga acaaagcgca ggttgtcgcg attaatcgcg
     6601 gaagtttttg aactggaacg gctgaccgag atcgccacca cgtaccatcg ccatgacagc
     6661 ctgcaaatca tcacgagatt tacccgttac gcggatctca tcgccctgaa tttgcgcctg
     6721 cactttcagt ttgctgtctt tgatcatttt gacgattttc ttctgagtcg cgctctcaat
     6781 gccctgtttc agtttcgctt ccacaaacca ggttttaccg ctatgaacga tattttccgg
     6841 tacatccagc gaactgcctt caatgccgcg cttcagcagc ttggcacgca gaatatccag
     6901 caactgattg acctggaaat cggactcgct caacactttg atggttttgc tggcgtcgtt
     6961 cagctcaaat gaggcttcaa cattacggaa gtcaaaacgg gactccactt cgcggctcgc
     7021 gttatcgacc gcgttacgtg cttcctgaag atcaacttca gagacaatat cgaaagatgg
     7081 catcttttcc tctcccttca tttttgatgc gaagcataat acctgcaaag ttaaataacc
     7141 aacagtcctg caccaggacg tatcccttaa ttgtgcatag tcgcaacagc atgcacaatt
     7201 aagggatagc cttattgaac acccggagtg gttgcgggtg aggaggaaca atgaaaatta
     7261 ccgtattggg atgcggtgcc ttagggcaat tatggcttac agcactttgt aaacagggtc
     7321 acgacgttca gggctggctg cgcgtaccgc aaccttattg tagcgtgaat ctggttgaga
     7381 cagatggttc gatatttaac gaatcgctga ccgccaacga tcccgatttt ctcgccacca
     7441 gcgatctgct cctggtgacg ctgaaagcat ggcaggtttc cgatgccgtc aaaagcctcg
     7501 cgtccacact gcctgtaact acgccaatac tgttaattca caacggcatg ggcaccatcg
     7561 aagagttgca aaacattcag cagccattac tgatgggcac caccacccat gccgctcgcc
     7621 gcgacggcaa tgtcattatt catgtggcaa acggtatcac gcatattggt ccggcacggc
     7681 aacaggatgg cgattacagt tatctggcgg atattttgca aaccgtgttg cctgacgttg
     7741 cctggcataa caatattcgc gccgagctgt ggcgcaagct ggcagtcaac tgtgtgatta
     7801 atccactgac tgccatctgg aattgcccga acggtgaatt acgtcatcat ccgcaagaaa
     7861 ttatgcagat atgcgaggaa gtcgcggcag tgatcgaacg cgaaggacat catacttcag
     7921 cagaagattt gcgtgattac gtgatgcagg tgattgatgc cacagcggaa aatatctcgt
     7981 cgatgttgca ggatatccgc gcgctgcgcc acaccgaaat cgactatatc aatggttttc
     8041 tcttacgccg cgcccgcgcg catgggatta ccgtaccgga aaacacccgc ctgtttgaaa
     8101 tggtaaaaag aaaggagagt gaatatgagc gcatcggcac tggtttgcct cgcccctggt
     8161 agtgaagaga ctgaagccgt caccactatc gatctgctgg ttcgcggcgg tatcaaagtc
     8221 accactgcca gcgtcgccag cgatggcaac ctggcgatta cctgctcgcg cggcgtgaag
     8281 ctgctggcgg atgcgccgct ggtcgaagta gctgatggcg aatatgacgt gatcgtgctg
     8341 cctggaggca ttaaaggcgc agagtgtttt cgcgatagca ccctgctggt tgaaaccgtt
     8401 aaacagttcc accgttccgg gcgtatcgtc gcggctattt gcgccgcacc agccaccgtg
     8461 ctggtcccgc acgatatctt cccgattggc aatatgaccg gcttcccgac gctgaaagac
     8521 aaaatacccg ccgaacaatg gcaggacaag cgcgtcgtct gggatgcacg ggtaaaattg
     8581 ctgaccagtc aggggccggg tacagctatc gactttggtc tgaaaattat cgacctgctg
     8641 gttgggcgtg aaaaagccca tgaagtggca tcacaactgg tgatggcggc agggatttat
     8701 aattattacg agtagtggcg gatgcggcaa acgtcgcatc tgaccagatg cgacgtaaaa
     8761 accacaaatt acgggcgata taccttcaca ttgttaaagc cctgctcgcg cagatagagc
     8821 gcctgcaggc ggctcatcac cccgcgctca caccacaaca gccaggtttt gttctggtcg
     8881 agatcgccaa atttggtgct cagtttatag aacggcagag aaaccacatc aatcccttcg
     8941 actttcagcg gcttatcttc ctgttcatcg atagaacgga tatcgaggat cacgtcgttc
     9001 gggccgaagc cattgacggt ttccacttcc accacttcct gctcggtctg ctgggcgatt
     9061 tcgcggatat caacgttatt cgcttcctca accactttat cgagaatgct gaagtcgaac
     9121 ttctcttctt ccgcttcaat cttcgattta accgctttca ccgtcgggct tttggagatc
     9181 acaccgcaat attcgggcat cgtgcgggca aagtcttcag tgccaatctg gcgagccagg
     9241 ttgatgatgt gctctttgtc gtaagaaatc agcggacgca ggatcagcgt gtcggagacg
     9301 ttatcaatca ggcgcaggtt agtcagcgtc tggctggaca cctggcccag tgcttcgcca
     9361 gtgaccagcg cctgtacacc gtaacgttca gccactttag acgcggcacg caccatcata
     9421 cgtttgagga taacacccat ctgaccgtcg tcgattttct cgagaatttc cccgacgaca
     9481 ggttcgaaat taatagcgac aaaacgcacg cggtgggagc tgccaaagcg gttccacaga
     9541 taatgcgcca cctgacgcac gccaatttca tgcgccgcgc cgccaaggtt aaagaagcag
     9601 taatgcacac gacatccgcg acgcatcaac atataactgg aaacgccgga gtcaaaacca
     9661 ccggaaatga gcgacagcac atcttcctgg gtaccaatcg ggaaaccgcc aataccttcg
     9721 tagcgacctt taatcagcag gagacgatcg tcttccactt ccagatggac agtcacatcc
     9781 ggattggtca gcttcacgcg cgcggactca atatgctgat ttaaaccgcc gccgacgtaa
     9841 cgttccacat caatcgagct aaaatcatgt ttgccacggc gcttcacgcg cacgcagaag
     9901 gttttgcctt ccagctgatc gcgatactga accaacgctt tctcgaaaat atcgtgcatg
     9961 tcggtaaacg gcacgtcttc gacttcgaga atatggtgga tgcccggaat acgggtcagg
    10021 gcgtcgcgaa tagccagacg ctggttttca tcttttgcgc gaacttcgat gttatcccag
    10081 tggcggacga cagcgagcgt ctcatcatag tgctttaaaa cgttacgaat gttcccggta
    10141 aggattttta taaagcgcaa gcgcacagat tggcttttga tggtgatttc cgggaacaat
    10201 ttaatgataa acttcatggc ggcaatggtt cgttggcaag ccttaagcga cttgtatagg
    10261 gaaaaataca gcagcccaca cctgcggctg catccaggcg cggaagtata ccactaacat
    10321 cgctttgctg cgcacatcac cttaccattg cgcgttattt gctatttgcc ctgagtccgt
    10381 taccatgacg gggcgaaaaa tattgagagt cagacattca ttatgccgaa gaaaaatgag
    10441 gcgcccgcca gctttgaaaa ggcgctgagc gagctggaac agattgtaac ccgtctggaa
    10501 agtggcgacc tgccgctgga agaggcgctg aacgagttcg aacgcggcgt gcaactggca
    10561 cgtcaggggc aggccaaatt acaacaagcc gaacagcgcg tgcaaattct gctgtctgac
    10621 aatgaagacg cctctctaac cccttttaca ccggacaatg agtaatggac tttccgcagc
    10681 aactcgaagc ctgcgttaag caggccaacc aggcgctgag ccgttttatc gccccactgc
    10741 cctttcagaa cactcccgtg gtcgaaacca tgcagtatgg cgcattatta ggtggtaagc
    10801 gcctgcgacc tttcctggtt tatgccaccg gtcatatgtt cggcgttagc acaaacacgc
    10861 tggacgcacc cgctgccgcc gttgagtgta tccacgctta ctcattaatt catgatgatt
    10921 taccggcgat ggatgatgac gatctgcgtc gcggtttgcc gacctgccat gtgaagtttg
    10981 gcgaagcgaa cgcgattctc gctggcgatg ctttacaaac gctggcgttc tcgattttaa
    11041 gcgatgctga tatgccggaa gtgtcggatc gcgacagaat ttcgatgatt tctgaactgg
    11101 cgagcgccag cggtattgcc ggaatgtgcg gtggtcaggc attagattta gacgcggaag
    11161 gcaaacacgt acctctggag gcgcttgagc gtattcatcg tcataaaacc ggcgcattga
    11221 ttcgcgccgc cgttcgcctt ggtgcattaa gcgccggaga taaaggacgt cgtgctctgt
    11281 ctgtactcga caagtacgca gagagcatcg gccttgcctt ccaggttcag gatgacatcc
    11341 tggatgtggt gggagatact gcaacgttgg gaaaacgcca gggtgccgac cagcaacttg
    11401 gtaaaagtac ctaccctgca cttctgggtc ttgagcaagc ccggaagaaa gcccgggatc
    11461 tgatcgacga tgcccgtcag tcgctgaaac aactggctga acagtcactc gatacctcgg
    11521 cactggaagc gctagcggac tacatcatcc agcgtaataa ataaacaata agtattaata
    11581 ggcccctgat gagttttgat attgccaaat acccgaccct ggcactggtc gactccaccc
    11641 aggagttacg tctgttgccg aaagagagtt taccgaaact ctgcgacgaa ctgcgccgct
    11701 atttactcga cagcgtgagc cgttccagcg ggcacttcgc ctccgggctg ggcacggtcg
    11761 aactgaccgt ggcgctgcac tatgtctaca ataccccgtt tgaccagtta atctgggatg
    11821 tggggcatca ggcttatccg cataaaattt tgaccgggcg ccgcgacaaa atcggcacca
    11881 tccgtcagaa aggtggcctg cacccgttcc cgtggcgcgg cgaaagcgaa tatgacgtat
    11941 taagcgtcgg gcattcatca acctccatca gtgccggaat tgggatcgca gttgctgccg
    12001 agaaagaagg caaaaatcgc cgcaccgtct gtgtcattgg cgatggcgcg attactgctg
    12061 gcatggcgtt tgaagcgatg aatcacgcgg gcgatatccg tcctgatatg ctggtggttc
    12121 tcaacgacaa tgaaatgtcg atttccgaaa atgttggcgc gctcaacaac catctggcgc
    12181 agctgctttc cggtaagctt tactcttcac tgcgcgaagg cggaaaaaaa gttttctctg
    12241 gcgtgccacc cattaaagag ctgctcaaac gtaccgaaga acatattaaa ggcatggtag
    12301 tgcctggcac gttgtttgaa gagctgggct ttaactacat cggcccggtg gacggtcacg
    12361 atgtgctggg gcttatcacc acgcttaaga acatgcgcga cctgaaaggc ccgcagttcc
    12421 tgcatatcat gaccaaaaaa ggtcgtggtt atgaaccggc agaaaaagac ccaatcacct
    12481 tccacgccgt gcctaaattt gatccctcca gcggttgtct gccgaaaagt agcggcggtt
    12541 tgccaagcta ttcaaaaatc tttggcgact ggttgtgcga aaccgcagcg aaagataaca
    12601 agctgatggc gattactccg gcgatgcgtg aaggttccgg catggtcgag ttttcacgta
    12661 aattcccgga tcgctacttc gacgtggcaa ttgccgagca acacgcagtg acctttgctg
    12721 cgggtctggc gattggaggc tacaaaccca ttgttgcgat ctactccacc ttcctgcaac
    12781 gcgcctatga tcaggtgctg catgacgtgg cgattcaaaa gcttccggtc ctgttcgcca
    12841 tcgaccgtgc gggcattgtt ggtgctgacg gtcaaaccca ccagggcgct tttgatctct
    12901 cttacctgcg ctgcataccg gaaattgtca ttatgacccc gagcgatgaa aacgaatgtc
    12961 gccagatgct ctataccggc tatcactata acgatggccc gtccgcggtg cgctacccgc
    13021 gcggcaacgc agttggcgtg gaactgacgc cactggaaaa actgccaatt ggcaaaggca
    13081 ttgtgaagcg tcgtggtgag aaactggcga tccttaactt tggtacgctg atgccagaag
    13141 cggcgaaagt cgctgaatcg ctgaacgcta cgctggtcga tatgcgtttc gtgaaaccgc
    13201 ttgatgaagc gttaattctg gaaatggccg ccagccatga agcgctggtt accgtagaag
    13261 aaaacgccat tatgggtggt gcaggcagcg gcgtgaacga agtgctgatg gcccatcgta
    13321 aaccagtacc cgtgctgaac attggtctgc cggacttctt tattccacaa ggaactcagg
    13381 aagaaatgcg cgccgaactc ggcctcgatg ccaccggtat ggaagccaaa atcaaagcct
    13441 ggctggcata atccctactc tactcctgct atgcttaaga aattattcat ggcaggagtg
    13501 gcagcatgca atacaacccc ttaggaaaaa ccgaccttcg tgtttcccga ctttgcctcg
    13561 gctgtatgac ctttggcgag ccagatcgcg gtaatcacgc atggacactg ccggaagaga
    13621 gcagccgtcc catcattaaa cgcgcactgg aaggcggcat aaatttcttt gataccgcca
    13681 atagctattc cgatggcagc agcgaagaga tcgtcggtcg cgcactgcgg gatttcgccc
    13741 gtcgtgaaga cgtggtcgtt gcgaccaaag tgttccatcg cgttggtgat ttaccggaag
    13801 gattatcccg tgcacaaatt ttgcgctcta tcgacgacag cctgctccgt ctcggcatgg
    13861 attatgtcga tatcctgcaa attcatcgct gggattacaa cacgccgatc gaagagacgc
    13921 tggaagccct gaacgacgtg gtaaaagccg ggaaagcacg ttatatcggc gcgtcatcca
    13981 tgcacgcttc gcagtttgct caggcactgg aactacaaaa acagcacggc tgggcgcagt
    14041 ttgtcagtat gcaggatcac tacaatctga tttatcgtga agaagagcgc gagatgctgc
    14101 cgctgtgtta tcaggaaggc gtggcggtga ttccatggag tccgctggcg cggggccgtc
    14161 tgacgcgtcc ttggggagaa accaccgcac ggctggtgtc ggatgaagtg gggaaaaatc
    14221 tctaccaaga aagcaatgaa aatgacgcac aaattgcaga acgcttaacg ggcgtcagtg
    14281 aagaacttgg tgcaacgcga gcacaagttg cgctggcctg gttgttgagt aaaccgggca
    14341 ttgccgcacc gattatcgga acttctcggg aggaacagct tgatgaactg ctaaacgcgg
    14401 tggatatcac cttgaagccg gaacagatcg ccgagctgga aacgccgtat aaaccgcatc
    14461 cggtcgtagg atttaaatag gtatgcaggc ctgatgagac gtgacaagcg tcacatcagg
    14521 catcggtgca caactacgac agaataccca gcggccagtg atgaccgata aaatacagga
    14581 tgccagcgga gatcaccccg gcgacaatat cgtcaatcat gatccccatg ccgccatgta
    14641 cattgtgatc aaaccagcgg atcggccacg gcttccacat atccagaata cggaaaatca
    14701 caaatccggc ggtaacccac tgccagtcat tggtcggcag cgccatgagc gtaatccaca
    14761 taccaataaa ttcgtcccag acaatactgc cgtgatcgtg cacgcccatg tctttcgccg
    14821 tttgatgaca aagatagacg ccgatacaga tccccagcat taccaccagc gaatagagct
    14881 gccagggcaa aaaagtcatc agataccaga agggaatcgc cgccagcgac cccatcgtgc
    14941 caggaacgat cgggcttaat ccacttccga atccgacagc aagcagatgc cacggattac
    15001 tcattttcag gcgacttttc gcgacatctt tatggcgtgg caaaatggtc atatcctttc
    15061 cagtctaacg tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc
    15121 atttgcccga tacaggtaaa cggtacgcct aggtgtccga gagccacatc cagcgcgcca
    15181 cggttcagtt ccggcaccgt gaaacacaac tcgtaatctt caccaccaga gagcgcccaa
    15241 cgcagcgcct gttccggttc aacatggcga gaaagcgcat cagaaaacgg cagcaatgcc
    15301 aggtcaatac gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga
    15361 ccgtcagaga gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata
    15421 cgcggcgacg gacggagatg acgtttgatc aagtagtccg catctttagc atcggcaacc
    15481 tgcaaacggt tttgcaaaat cgccagcccg gcagcgctat cgcccggtgt accggtcaca
    15541 tagatccagt cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag
    15601 ccgtggatac ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc
    15661 atatcgtaat aattgagaag atcaaacaag ctgtcgctga aggactcaag ccacgcttcg
    15721 tctacgtccg gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc
    15781 agatcgctta ggttcaccgc cagtgcttta taagccagat cagcaggatc gatatcaggg
    15841 aggaagtggt tgcccgccac cagcgtatca gtgctgatcg ccagggtctg tttctcgggg
    15901 atattgagaa gagcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt
    15961 acacggtcaa aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca
    16021 gcaggtaaaa aagaaaaggc cggcgatcat ggaacggtct tccgtgaatc taccggcctg
    16081 gatattactt tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga
    16141 acttatggct gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca
    16201 ctttgtatgg cacatcgcta cgtttagaca gctcgtacag cgcaatgcgc agtactgctt
    16261 tttctacctg gcccagttct tccagcagac gggacaggta cggcttcatc agtccgtcga
    16321 ggtatgcggt gttagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt
    16381 cttttacatc ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca
    16441 actgccagga gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt
    16501 tcacggattt ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca
    16561 agcgcggtca gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc
    16621 gcttgttcaa tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg
    16681 gcaacatgcg ccaggccgtt gcttgcacca ccagcgacat attcaaagtg ggcagtgcca
    16741 ccacgaataa ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca
    16801 cccgccgcca gcggcagctc ataggcacct ggcacccaaa caacggtaat gttttcatct
    16861 tttacctgac cgatacgttt cagtgcgtca attgcacctt ccagcaggct gtcattgata
    16921 aagttgttga aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct
    16981 tcaataatgt tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt
    17041 agcataatat ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg
    17101 caggcaaaca tccgggccta catgacgtat ctctttgaat ttaaattggg gagcgtcggc
    17161 taatttctca agccctggca gcgtgcataa tccacgggcg tcgttgccta atagtttagg
    17221 tgcgatatag acaatcagct catcgactaa acccgcctgt agcaatgcgc cagcgagcgt
    17281 tggccccgct tccacccaga tgctgttaat ctgctgttta cccagttgca tcattagtac
    17341 aaccagatcc aggtgacctt tatgctctgg aatcagcaag gtacgcaccg tttccggcca
    17401 ctcacgagaa tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgcg
    17461 atgttccggc gtcacgcgat tttggctatc aatcacaata cgtaccggct ggcggagatt
    17521 ttgttgcggg tagagtccct gagtttgttc atccagttca gaccaacgca ctgttaaggc
    17581 tggatcatcc gccagcaccg tggcgctgct ggttaaaatg gcatgacttt gcgcgcgcag
    17641 tcgttgtacg tcgcgcctcg cctgaggtga agtgatccac tggctttcgc cactcgccat
    17701 cgccgtgcga ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg
    17761 catccgtttg agaaagcctt tattcaattg ctcagcttca ctcatcatca gcccgtggct
    17821 gacgtcaatg ccagcctgtt gcagacggta aagtccacgt ccagcgacct gcggattggg
    17881 gtcttgcatt gcagcaacca cgcgcgccac gcccgcagca attaatgcat cacaacacgg
    17941 tggcgtacga ccatgatggc tacagggttc gagtgtgaca tacgccgttg cgcctttggc
    18001 tttttcgccg gccatgcgca acgcgtgtac ctcggcatgc ggttcacccg cgcgttgatg
    18061 gtaaccttca ccgacaattt cgccatcttt gacaatgacg cacccgacat tcgggttggg
    18121 atgcgtggta aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc
    18181 ctgcacggct tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa
    18241 actgcggtag acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc
    18301 catcaccaga ttgccaatca tcttgctcgg cacttcgcgc tcaccggtgg cgcgcagctg
    18361 cgattttata tgattgattg ccatttcgac gtcatcggaa ctcaccggac gtttttccag
    18421 cgcccgcagc attccgctac gtaatttctc ttcattaaac ggttcacgca cgtcgttgct
    18481 ttttacaaca cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca
    18541 caccagacac tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat
    18601 taccttagtg tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt
    18661 taacagaaat ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat
    18721 aaaggatcga tgctttcact gcttttaccc tctaccatta ggctaattgc gacgagaaag
    18781 ggacgaccta tgaatactaa cgtttttcga ctgctcctgc tgggaagcct gttcagcctt
    18841 agcgcctgtg tgcagcaaag tgaagtgcga cagatgaaac acagcgtcag cacgctgaac
    18901 caggagatga cgcaactcaa taaagaaacg gtcaaaatca ctcagcaaaa caagctgaat
    18961 gcaaaatcca gcagtggggt ttaccttctg cctggagcaa aaacaccagc aagactggaa
    19021 agccagatcg gtactttacg tatgtcgctg gtgaatatta cgcctgacac agatggcact
    19081 accctgacgc tacgtattca gggcgaatcc aatgaccctt tacccgcatt cagcggaacc
    19141 gttgaatatg ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtgcagaat
    19201 caattgatta acgcacctgc cagcgtcctc gcccccagcg atgttgatat tccgttacag
    19261 ttaaagggta tgtctgtgga tcagttaggc tttgtacgta tccacgacat tcaacctgtt
    19321 atgcagtaaa cgtattgcgg ggcgatattg tgcgtcccgc aacatctttc tccgtcattt
    19381 tgttactccg cttacatcac ctggattgat agtaaaagtt tgcaacaagg gtgaaagtca
    19441 gtacaatccc cgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa
    19501 ctgtgaaacg aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg
    19561 aaatatttag aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg
    19621 catacatatg aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt
    19681 tactgtcaac gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag
    19741 cgttaacgtt gtcggaagct atcacacccg tttcggaccg cagctccgca acgataccta
    19801 ccttgagtac gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc
    19861 gccggtattc ttcggcggta actccgatgc aaaaggtatc tggaaccacg gttctccgct
    19921 gtttatggaa atcgaaccgc gtttctccat cgacaagctg accaatactg accttagctt
    19981 tggtccgttc aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa
    20041 agatggccgc cagagcacct ggtacatggg tctgggtacc gacatcgata ctggtctgcc
    20101 gatgagcctg tccatgaacg tctatgcgaa ataccagtgg cagaactacg gcgcagcgaa
    20161 cgaaaacgag tgggacggtt accgtttcaa agtgaagtac tttgtgccga ttaccgatct
    20221 gtggggcggc cagctgagct acatcggctt caccaacttc gactggggtt ccgacctggg
    20281 cgatgacagc ggttatgcga acaacggtat taaaacacgt accaataact ctattgcgtc
    20341 cagccatatt ctggcgctga attacgatca ctggcactac tctgtcgttg cccgttactg
    20401 gcataacggt ggtcagtgga atgacgatgc agaactgaac ttcggcaacg gcaacttcaa
    20461 cgttcgctct accggctggg gtggttatct ggtagtaggt tacaacttct aattgtgaga
    20521 atgccggatt tcatatccgg cattttttat cacttcttct tgttcatcat cgctttcaaa
    20581 tcagcgaatg ggttgtactt cgcttcaccg acatctttct gcgcatcttc ccctgcgata
    20641 acggtcgtac catactgatc cgcttcggtg tatttcgaat gctcatgatc gtggcaatag
    20701 agacaaagta attcccagtt actgccatct tccgggttat tagtatggtc gtgatcaatg
    20761 tggtgaacgg taagttcacg caggttggaa taaacaaact cgcgggaaca gcgaccgcag
    20821 acccacggat agatttttaa tgctttttcg cgatagccgc tttctaaccg cgcatagttt
    20881 tttgggatga tagccatgaa aaagttacct ggatgaaatg tagggttacg cgcagtttcc
    20941 caagatgcgg ggattttcgc aatcacctta catcaaaaaa aatcccgatc ttctgaccgg
    21001 gattttcaac atcagctgaa acttgattaa ggcagaattg acggctgatc cgccccttct
    21061 ttttccactt tctgctgcaa catgtgttcg cgcttcatac ccagtttcag agccagcgcc
    21121 gatgccacat agatggaaga tgcagtaccg atggaaacac cgataagcat ggtcagcgag
    21181 aagccttcca gtaccggacc accgaagagg tacagcatca ggataaccat caaggtagta
    21241 ccggatgtga tcaaggtacg gtgcagcgtc tgggtcaagg acacgttaaa gatttcgtaa
    21301 ggcgtaccgc gacggatctt gcggaagttt tcacgaatac ggtccgatac cacgatactg
    21361 tcgttaagcg agtaaccgat aactgacatc aacgatgcca caatggtcag gtcaatctcg
    21421 atatggaata acgacaaaat acccagcgta atgataacgt cgtgtgccag cgcaataaca
    21481 acccctgccg ccagtcgcca ctcaaagcgg aaacctacgt acacgaggat agacagcagc
    21541 gccgccatca acgccatcgc accggtttgc gcaaggtctg cccccacgct cggaccgacg
    21601 aactcaatac gcttcactgc cgcattctga ttggtggatt cgttaatcac cttcaggacc
    21661 tggctgccca gcacctgacc gccggtttcg ccttcagcag gcggcatacg gaccatgatg
    21721 tcgtggctgc taccaaagtt ttgcagcatc ggctcttcaa aaccggcttt ttgcaatgca
    21781 tcacgcatta cgtcaatttc agccggtttt tcgagcgtaa tttcaataac cgtaccaccg
    21841 gtgaaatcca gcccccagtt aaagccgcgc acgcccataa taacgatagc ggcgattaac
    21901 agcagaccag agatgccaaa agcccagtag tcccagcgca taaagtcata gactttacgg
    21961 ccgtggttta gttgttcaac agtatattcc tgtgccacat cgcactcctc agattgacag
    22021 cttcttgacg cgcttgccgc catatagcag gtttacgatg gcacgcgtac cgacaatcgc
    22081 ggtaaacatc gacgtcgcca caccgatacc ggtagtaatc gcgaaccctt taattgcccc
    22141 ggtacccact gcgtacagga tgatgacttt aatcagcgtg gtgatgttcg catcgaagat
    22201 agaactgaat gcgccacgat aaccttcatc aattgcctgt tgaacagtac gtccgttgct
    22261 caactcttct ttaatacgtt cgttgatcag tacgttcgca tcgaccgcta ccgcaagggt
    22321 taagacgata cccgcgatac ctggcatact cagcgttgcg cctggcaaca gcgacataat
    22381 accgacaatc aagatcaagt tggcaatcag agcactggtc gcaatcagac caaacttctt
    22441 atagaagatg atcatgaaca gaatagaaac cagcagaccg gcaaggcaag cctccagccc
    22501 ctgctcaatg ttctgcatac ccagggttgg gccaatggta cgttcttcaa caatctgaat
    22561 cggcgcgatc aacgcaccgg cacgcagcag cagtgaaagc tgacgcgctt cgcttgggtt
    22621 attgatgcca gtaatacgga agctgttacc cagacgagac tggatgttgg cgatattaat
    22681 cacctcttcc tgtttcacca gaaccgcacg accgtttgca tctttcttac cgctgtcttt
    22741 gtactccaca aacagggttg ccatcggttt gccgatgttg tccttggtga agttagacat
    22801 gatgttacca cccgcgctat cgagcgagat gttaacctgc ggctggttgt attcgtcctg
    22861 gctggaagtg gagtcggtga tatggtcacc ggtcagaatt acgcgtttgt acagcacaac
    22921 cggctgacct tcgcgggtct gtttcacttc agagtcgccc ggtacgcgac cggatgccgc
    22981 agcggcctga tcaacgttgg tgtttaccag acggaattcc agcgttgccg tcgcacccag
    23041 aatctctttc gcacgcgctg tgtcctgaat acctggcagt tcaacaacga tacggtcagc
    23101 accctgacgc tgaaccaccg gctccgccac gccaagttgg tttacacggt tacgcaggat
    23161 attaatgttc tgctgcaccg catattcacg cgcttcactc aggcgagcat cgctcattac
    23221 cgcacgcagc tggttgctgc cctggctgct aatcaccagg tccggatggc gcttgctcag
    23281 atacgcaatg gcttcatcac gagctttagc atcgcggaaa gtgatgctca ggccgtagtt
    23341 gttttcttta cgaacggtgg tatacgggat gcccttttcg cgcaggtcgc tgcgcaggct
    23401 atcgatgttt tgttcctgga gtttgccaag cgcggtgtcc atatccactt ccatcaggaa
    23461 gtgaacgccg ccacgcaggt caaggccgag cttcatcggc tcagcgtgaa tagctgccag
    23521 ccagcgcggc gttgccgggg caaggttaag cgccacgacg tatttgtcac ccataacgcc
    23581 cattaatgct tcacgagcgc gcagctgggt gtcagtggag tcgaagcgcg caagaatagc
    23641 gccctcttcc agtgccacag acttagcagt tattttttct tcttgtaacg ttttctggac
    23701 ctggatcagc gtttgctcac tggcggcgac tccgcgcgca ccagtgatct gaacagccgg
    23761 atcctcacca aacaggttgg gaagcgcata cagcagaccg atgacaatca ccacgatcag
    23821 catgacgtac ttccacaaag gataacggtt taacacggca attcccttag ggaaaaattt
    23881 taattacagc gccttcatgg tgcctttcgg cagaacggca gctacgaagt cacgtttaat
    23941 aactacttca gtggtgtcat tcagcgcgat agcaatgtag ccgttttccg ctactttggt
    24001 tacgcgaccg accaggccac cgttcgtcag cacttcatca cctttagcga tggagtccat
    24061 cagctttttg tgttctttgg tgcgcttctg ctgtggacgc aggatcatga aatagaaaat
    24121 cagaccgaat accaccagca tcaaaatcaa agacatcggg ctaccttgcg ccggtgcacc
    24181 cgttgccgct accgcatcag aaataaaaaa gctcattaaa tttccctcat tattaatatt
    24241 aatcaacgtt caaaggtggt acttctcgcc cctgacgctg gtaaaaatca gttacgaagc
    24301 tctctaattt accctcttca atagccttgc gtaaacccgc catcaaacgc tggtagtaac
    24361 gaaggttatg aatggtattg agtcgcgcgc ctaatatttc gttgcaacgg tcgagatgat
    24421 gcaagtaagc gcgtgaataa ttgcgacagg tgtagcaatc acactcagga tcgagtgggc
    24481 cagtatcgct cttatacttc gcattgcgga ttttcaccac gccatcggtc acgaacaaat
    24541 gaccatttcg ggcgttgcgg gttggcatta cgcagtcaaa catatcgata ccgcgacgca
    24601 cgccttcaac caggtcttct ggtttaccaa cgcccatcag gtaacgcggt ttgtctgccg
    24661 gaatttgcgg gcatacatgc tccagaatgc ggtgcatatc tgctttcggc tcacccacag
    24721 ccagaccgcc gacagcgtag ccatcaaaac cgatatctac cagaccttta acagaaatat
    24781 cacgtaaatc ttcgtaaacg ctgccctgaa tgataccaaa cagtgcattt ttgtttccga
    24841 gactgtcaaa acgctcacgg ctacgcttcg cccaacgcag agacatctcc atggagcgtt
    24901 ttgcgtaatc ccagtcagca ggatacggcg tacactcatc aaagatcatg acgatatccg
    24961 aaccaagatc gtactgaatc tccatcgact tttccgggtc gaggaaaatc gggtcgccgt
    25021 tgatcgggtt acggaagtgc acgccctgtt cggtgatttt acgaatatca ccaaggctga
    25081 agacctggaa gccgccggag tcggtaagaa tcggcccttt ccactgcata aaatcgtgta
    25141 aatcgccatg cagtttcatg atttcctggc ccgggcgcag ccacaggtgg aaggtgttac
    25201 cgagaataat ttgtgcgcca gtggcttcaa cttcttccgg cgtcatccct tttacggtgc
    25261 cgtaagtgcc aacaggcata aaacaaggcg tttccactac gccacgatca aagaccaggc
    25321 ggccacggcg tgcgcgaccg tcggtggtgt ccagttcaaa tttcattttt tctccactac
    25381 gtcagaaaaa cagtccaacg tttaaaccag cgccgcggaa ttactcccca acgcgctcat
    25441 taattgcctg cggattgtac gtgataaaca tcgcatcacc gtaactaaaa aagcgatatt
    25501 tctcttctac cgccgcttta taggcattca tggtgtgttg ataaccagca aatgccgaaa
    25561 ccagcataat cagcgtcgat tccggaaggt ggaagttcgt caccagcgca tcgaccactt
    25621 tgtactggaa acccggatag ataaagattt gggtatcgtc aaagaacggt tcaatgagat
    25681 cgtttttcgc tgcctgagcc gcactttcca gcgaacgtac tgaggtggta ccaaccgcaa
    25741 tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc taccacatcc tgcggcactt
    25801 cagcgtattc cgagtgcatg atgtgatctt caatggtgtc gacgcgcacc ggctggaagg
    25861 tgcccgcacc aacgtgcaac gtcacaaacg ccatctccac gcctttggcg cgtaattttt
    25921 ccagcaaagg ttcgtcaaag tgcagtccgg cggtcggtgc tgctaccgcg ccgggttttt
    25981 cgctataaac cgtttgataa agttcgcggt ctgcatcttc gtccggacgg tcgatgtacg
    26041 gcggcagcgg catatggccg atgctgttga gaatatccag caccgaacgt tgatcattaa
    26101 attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt tgcgttaata cgttcgtcat
    26161 cgcccagcag cagttctgcg ccaggttttg gcgctttcga ggcgcgaata tgcgcaagaa
    26221 tgcgtttgtc gtcgagcatc cgctcaacca gcacttcaat cttgccgccg ctggctttac
    26281 gcccaaacag acgcgccggg atcacgcggg tattattaaa aaccagaaga tcgccggggt
    26341 tgagcttatc gagtaaatcg gtgaaagtac cgtgcgtcag cgcgcccgtc ggcccgtcca
    26401 gcgacagtaa acgacagcta ctgcgttcag gcatgggata gtgggcaatc agggattcgg
    26461 gcaattcaaa agagaaatcg gtaacgcgca tgacactgac tcagactaaa ataagaggcg
    26521 ggtagtctag tgccggggcg ctctccctgc aacctttacc cccctttgga tagaatataa
    26581 tcgatgaatt ttttagctca cctgcattta gcccatctcg cggaaagctc gctttccggc
    26641 aatttactgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg
    26701 gctggcattc atatgcatcg gcgaatcgac gtattaaccg acaatctgcc agaagtccgc
    26761 gaagcgcggg agtggtttcg taatgaaacg cgccgtgtcg cgcctattac gctggatgtg
    26821 atgtgggatc actttctgtc ccgccactgg tcgcagctgt cgcccgattt tccgctacag
    26881 gaatttacct gttatgcccg ggagcaagtg atgactattc ttccagactc accgccacgc
    26941 tttatcaatc tgaacaacta cttatggtca gagcagtggt tggtgcgcta tcgcgatatg
    27001 gatttcatcc agagcgtgtt aaacggcatg gcaagccgtc gcccacgtct ggatgccctg
    27061 cgtaactcct ggtacgattt agacgcccat tacgatgctc tcgaaacctg cttctggcag
    27121 ttttatccgc ggatgatgga gcaggcgtca cgcaaggcgt tataagacgt taattcatcc
    27181 acaccgtagc cgaaatggca ggcaaagcca gaatcccgtc tgtcagttgc ccgtgacctt
    27241 ctttgcgttg ccattgcgcg acattaagca acggcgacgc gggtagcacc acttcacagg
    27301 cttcgccacg gttgattgcc accagtacac gctgctgata cagcacgcgg acaaatacca
    27361 ccacgttatc ttccgcatac agtacctgac agccgccacg acgcagcgcc tgacttttct
    27421 tacgcagcgc aatcattcgc tggtacagcg cgaataacgc cgtatcctgc ttttccacct
    27481 gccaggggaa cggtttacgg caaaacggat cgtttttacc atccagtccc acttcatcac
    27541 cgtaataaat gcacggtaca ccaggccagg tgaacaacca gaccaccgcc agcggcaggc
    27601 gcgcaatatc ccgaccgagc agcgttttaa atcgcgcagt atcgtggcta tcgagctgat
    27661 taaacatacg cagttgctgt tgatgagaaa gtccggcgcg gtaattatcc atccaggcca
    27721 tacaggtttg ggcgtcaatt tgctgcggat cgtaagagat gtcggtattg gcaagaaatc
    27781 cccacaacgg gaatgtgaag ccgcgatagt tcatggcggc atcttccaca tcggcctgta
    27841 accattgccg tgcatcgcca aaatgctctc caacgatata cgcttccggc tgggtttctt
    27901 tcgccgcttc ggtgatcccg gctacgtgct gcaaattatt gcgcgcccca cccgcttccc
    27961 ccagcatatg caccacatcc agccgccagc cgtccatatt ccacggcgct ttcagccagt
    28021 ggcggacgat actgtcttcc ccacgataaa tttcattcac cagactttcc gactgataat
    28081 ccagtttcgg caagctggca tagcccagcc agtcgagcgc cgtgccatcg tcactaaacg
    28141 agtaccagtc gcgccagggc gattcggggt tgtgacaagc accacccgtc ccgcgattgt
    28201 gtctgtcaaa ccaggcatgg gaatcgccac tgtggttaaa cacgccgtcc agcaccagcc
    28261 gcattcccag ctgctgcgta ttgtgtcgca aacgcaacaa cgccccatca ccgccaaact
    28321 gcggatcgac atggcgataa tcctcggtat cgtatttatg tacgctggga gctttaaaca
    28381 ccggattgag atacagcgcc gtcacgccaa gttttttcag atacggcagt ttttcgctta
    28441 tcccgtctag atcgccgcca tagaacgttg atccgcccgc ctgcgccgtg accggttcat
    28501 cccagtcacg caagatgatc tcttgtccgg ctgcgtgatg gtaatagaca tgatcctgtt
    28561 cagcttcacg aggaagacta cgggcgaaac gatcagggaa gatctgataa aaaatctgat
    28621 ccgcagccca ttgtgggccg atatccggta catcgacggc aaactgctcc agtcgtgccg
    28681 gcggcattcg gctgaagccc tgcggtgtaa accaacgctg gcgatcgtgc cacagtaatt
    28741 tgaaactata acgccgccga ggtagtccgc cggagagatc gatcgccgcc cgccatgcgg
    28801 taacaccagg ctgcggctga ctgcgctgct tatgcatcgg tactgacgtt tcttcgttgt
    28861 cgtgttctgt acgcagcata atgcgctgcg gtgggtcttc gcccgtcagc cacagtgtaa
    28921 tgagcagttg atctttgctt tgtttaacaa atgggggcac cggcaggtgc catgcattta
    28981 acatcataat tcccctgtga tgaaaatctg atcaccttgc catagcctaa tgcaggatca
    29041 ctcatcatct gaaggatatt tgggggagga ggacgggggc ggataacttt cggaatagcc
    29101 ggatcaggat ttcctcctga accggcttac agaacgcaag gattagtggt tttcagccag
    29161 ctgacgatcg tggcggcgct taaacatcca gccaatcaac agcacaacaa tccacgcgaa
    29221 accgacatac agcgagatac gagtatccgg gtgataacca atcaacccga taataaagag
    29281 caggaaaatc aaaccgccga tggtggttgc taccccaccc ggtactttaa atttcagcga
    29341 cttaacttct tctggcggta aacggcgacg gaaggcgatt tgcgacagca ggatcataat
    29401 ccacacccac accgtggcga aggttgccag cgaagcgatc accaggaaga cgttttccgg
    29461 catgatgtag ttcagataca cagcaaacag cagcgcggta gtcatcacca gcaccgtaac
    29521 ccacggaata ccgcgacgcg aggttttgct gaaaattttt ggtgcgctgc cctgctctgc
    29581 cataccgtgg agcatacggc ctacgccaaa tacgtcactg ttaattgccg acagcgaagc
    29641 ggtcagcaca acaaagttaa gaatgctggc ggcaaaggta atgcccatat gctggaacgt
    29701 cagtacgaac gggctaccgg cagtgccaac ctgattccac gggtagatag acataatgac
    29761 gaacagcgta ccgacgtaga acaccagaat acgcatcggc acggagttaa tcgcacgcgg
    29821 aatcgatttc tcaggatctt tcgcttcacc ggcggtaatc ccgataattt cgatcccacc
    29881 gtaagcaaac atcaccattt gcaacgacat caccatccca agccagccgt tactgaagaa
    29941 gccgccgttg ctccacaggt tatgaatacc ggtcggttgc ccgccgttgc caatccccca
    30001 gatgatgatg ccgaaaccgg cgacaatcat gatgatgatg gtggcgactt taaagaacga
    30061 gaaccagaac tccagctcac cgaatacctt cacgctcatc aggtttacgg cgcaaatgat
    30121 cagcaccacg ctcagtaccc aaatccagtg cggcaccgtc gggaaccaga cacccatata
    30181 gataccaaaa gcggtcacat cggcgatggc gacaataagg atttcaaagc agtaggtcca
    30241 gccggtaatg taaccagcca gcgggccgag gttttcctgc gcataacgcg agaaagagct
    30301 ggcggccggg ttatgtaccg acatttcccc cagcgcgcgc ataatgatat acgccgcgac
    30361 accaccgata atataggcca acaacacgct cggaccggcc attttgatgg cgtctgccga
    30421 accgtaaaac aacccggtgc caattgctga acccagtgcc ataaagcgta tgtggcgggt
    30481 acttagccca cgctttagct tgttcttact ttccatcaat tcctaaccca tcaacacaat
    30541 aaaaaaccac ggggcatcgc agccccgtgg ttaaaacaaa tgttcagtga tttagtgagc
    30601 gctggaggtc acctgacgac ctgccgcgcg atcccagata atggccagaa ccaccatcac
    30661 cactgttggc attaaccacg ccagaccttg ttcggccagc ggtaaacgct gcgcccagga
    30721 cggtaagata tcgctgaatg cagatgcttt gatcccgtcg agaataccaa aaagcaggct
    30781 gataaacatc ggcggagcaa tcacgcggga cgaattatgc caccatgagc gtgtaaaact
    30841 taatacaacc agtgcgatac acggcggata aatggcggtc agcaccggta cagagatctg
    30901 aatcagctga ctcaagccga ggttagacac caccatcgag aagccgccga ggataaacac
    30961 cagcgtacga taagagagcg gtacgtactg ggcgaagaat tctgcacaag cacaggtcag
    31021 gccaaccgcc gtgaccaggc aggcgatgaa gattaacgcc gccagcagga agctaccgcc
    31081 gccgccaaag gtatgctgaa cgtaagcatg caggatcgcc gcaccgtttg cagactgatc
    31141 gaccagcgac gcgctgtctg aacccagacg gaacagcgcc aggtacagca gagtcagacc
    31201 aacacccgcc atcaggccag cccagacggt ataacgggtc agcagacgcg cttcggtaac
    31261 gccacgagaa cgcgccgcgt taacaataac gataccaaac accattgcgc ccagcgtatc
    31321 catggtcaga tagccgttga cgaagccgtt agaaaacgca gcgttttgat aagcctcagt
    31381 agccgtgctg atagaacccg ccggccagat aattgcggca acagacagga tgaccagcgc
    31441 gataattttc agcggcgcaa ggaagttgcc cacggtatcc agcagcttgc ccggatagag
    31501 cgaaaccaga ataacgatag cgaaatagac caggctgtaa ataaacagcg gcagtgcgga
    31561 atcacccgtc agcggcgcaa tccccacttc aaaagaaacg gtagctgtac gcggcgtagc
    31621 gaacagcggc cccaccgcca ggtaacacac cgttgccagc agtacgccag caactttacc
    31681 gattggcgtg ctgaggctgt caacaccgcc gccaactttt gccagcgcca ccaccgttaa
    31741 taccggcagg ccaacggcag taatgaggaa gccgaatgcc gcagtccaga cgtgttcgcc
    31801 tgcctgcaag ccgaccattg gagggaaaat aatgttacct gcgccgacga acaacgcaaa
    31861 tgtcataaag cccagagcga tgatatcgcg cgatcttaat tgatgggtca taaatcatac
    31921 tgcctgtgga tgtggtgttg taacgttaaa tttttgtgcc aatccctggg gatgatacag
    31981 cggtatattt aaacaatttt agcgggaaat actcccaacc aggcgcggcg aagaatagtt
    32041 tttcgattta ccgcgttaat gcagtcagta tgacagcacc tgaagcgcaa tttaaacgct
    32101 tataacgttt taaagcaaga agggatctaa aaaccagagg tatatagcga taagaaatca
    32161 ccaaccagta taaaacacta tttcaatgaa aaagacatgg ctaatcatgc gaacaaaaaa
    32221 tcaccaaccg ttgtaaaatc taacgcccgt atttcgactg caaagaaaat aagccagcat
    32281 cggctggctt atggcaataa aagatgacaa aggcgaatta atcgctgttt ttggcaatta
    32341 aacgttccgg gataacaaaa ctgaaacgtg taacttttcc tactgtactc tcaatattca
    32401 ggcgactttc gtgatgattc accgcatgtt tcacgatcgc taaccctaat ccgctaccac
    32461 cggtttgccg ggaacgcgct ttatcaacgc gataaaaacg ctcggtcagg cgcggaatat
    32521 gctccggtgc aatgcccggt ccgttatctt caacactaaa ttcggcaccg tgcggcactc
    32581 gctgccagcg tacggtgata tgcgtgcctt ccggcgtatg attcacggcg ttatagacca
    32641 ggttcgaaat cgcactgcgt agctgatctt cgttgcctga caccttgagg ccgttatcta
    32701 tctcaaaggt aaatgtctgt tttttctgac tcagagtctg agcctcgcgc tcaacgacgc
    32761 gcagcatcat cggcacatca accttttcat tgagcaaatg cgtcggtgcg gcttctattt
    32821 tcgacagcgt cagcaattgc ttcaccaacc cttccatccg ctgggtctgc tcgcgcatgg
    32881 tgtgcaacgc tttttcgcgt accgcgcctt ccagcggctg ctcatccatc atctccaggt
    32941 aaccctgtaa cacggtcaat ggcgtacgta actcatggct cacgttggca aaaaagttac
    33001 gccgcgcccc ttccagttga tgcatttgcg tgacatcacg cgccaccatc agcaactgtt
    33061 tgtgggtata aggcataacg cgaatttcca gatgccgccc ggtgttgagc accagattaa
    33121 gcgggcgaga aaaatcacgc gttttcagat attgcgtaaa ctccgggtaa cgcagcaggt
    33181 taaggatgtt ctgcccgtta tcttccggcc agcgcaaacc aagaatctgt tgcgccagac
    33241 cgttacacca gaaaataccg ccctcttccg tggtcagcac tactgcgtcg ggcagcgact
    33301 ccgcgccact acgaaagcgt ttaatcagat tgcccagttc gcggcggcgt tttttatttc
    33361 gcagctgcat ctggtgtaag ccgtatagta gcggttccca gctaccacgc cccggtggcg
    33421 gggtcatact gcgatccacc cacagccacc atgaaaggcg caataaattc cagaaatgcc
    33481 agataagcag tcctgttacc gatgccagca aaaaccaggg caggtaacca aaaaatgcac
    33541 ccaggatgaa agccgggagg cagcaaagta gcagctccag caccagcctt ttccacgaca
    33601 gccgttccag cacgtaagat actccagtta agaaatcata agccctgctc tgcgtccgat
    33661 gagcaaggcg ttaaaagcgg gttgaaaaac gatatcctgt accgcgcacg gtctgcacca
    33721 tgcggtcatg cccgccgggc tccagtgctt tacgcaggcg acgaatgtgg acatcgaccg
    33781 tgcggtcttc cacatacacg ttagttcccc agacgtggtt taacagctgc tcgcggctgt
    33841 acacgcgctc aggatgcgtc ataaaaaagt gcagcagttt aaattctgtc ggccccatct
    33901 ccagtggctc ttcgcccgcc atcacccggt gagatgtcgg gtcgaggctt agcccctgca
    33961 tctcgatcac ctcttccacc gccattggcg aaatacggcg cattaccgct ttgattcgcg
    34021 ccaccagctc cttcggcgaa aacggcttgg tgatatagtc atccgcgccg gtttcaaggc
    34081 cgcgcacgcg atcttcttct tcccctctgg cggtcaacat caccactgga atatcccggg
    34141 tcatcgactc gcgcttgagg tgtttgatga actggatacc ggagccgcca ggtaacatcc
    34201 agtcaaggag aattaaatcc ggccagggtt cattcagttg attcacagca ctgtcataat
    34261 cttccgcttc gaccggctga aagccatttt gttcgagcac gaagcagacc atttcgcgaa
    34321 ttggagcttc atcttctacg accagaatac gtctcgccat gatttgccct gttgtaataa
    34381 ataggttgcg atcattaatg cgacgtcatt atgcgtcaga tttatgacag atttatgaaa
    34441 agctcgtcgc acatatcttc aggttattga tttccgtggc gcagaaaaaa gcaaatggca
    34501 catctgtttg ggtataatcg cgcccatgct ttttcgccag ggaaccgtta tgcgcatcct
    34561 tcacacctca gactggcatc tcggccagaa cttctacagt aaaagccgcg aagccgaaca
    34621 tcaggctttt cttgactggc tgctggagac agcacaagcc catcaggtgg atgcgattat
    34681 tgttgccggt gatgttttcg ataccggctc gccgcccagt tacgcccgca cgttatacaa
    34741 ccgttttgtt gtcaatttac agcaaactgg ctgtcatctg gtggtactgg caggaaacca
    34801 tgactcggtc gccacgctga atgaatcgcg cgatatcatg gcgttcctca atacaaccgt
    34861 ggtcgccagc gccggacatg cgccgcaaat cttgcctcgt cgcgacggga caccaggcgc
    34921 agtactgtgc cccattccgt ttttacgccc acgtgacatt attaccagcc aagcggggct
    34981 taacggtatt gaaaaacagc agcatttgct ggcggctatt actgattatt atcaacaaca
    35041 ctatgccgat gcctgcaaac tgcgcggcga tcagcctctg cccatcatcg ccacgggaca
    35101 tttaacgacc gtgggtgcca gtaaaagtga tgccgtgcgt gacatctata ttggcacgct
    35161 ggacgcgttt ccggcacaaa actttccacc agccgactac atcgcgctcg ggcatattca
    35221 ccgcgcacag attattggcg gcatggaaca tgttcgatat tgcggttccc ccattccact
    35281 gagttttgat gaatgcggta agagtaaata tgtccatctt gtgacatttt caaacggcaa
    35341 attagagagc gtggaaaacc tgaacgtacc ggtaacgcaa cccatggcgg tgctgaaagg
    35401 cgatctggcg tcgattaccg aacagctgga acagtggcgc gatgtatcac aggagccacc
    35461 tgtctggctg gatatcgaaa tcactactga tgagtatctg catgatattc agcgcaaaat
    35521 ccaggcatta accgaatcat tgcctgtcga agtattgctg gtacgccgga gtcgtgaaca
    35581 gcgcgagcgt gtgttagcca gccaacagcg tgaaaccctc agcgaactca gcgtcgaaga
    35641 ggtgttcaat cgccgtctgg cactggaaga actggaggaa tcgcagcagc aacgtctgca
    35701 gcatcttttc gccacgacgt tgcatagcct cgccggagaa cacgaagcat gaaaattctc
    35761 agcctgcgcc tgaaaaacct gaactcatta aaaggcgaat ggaagattga tttcacccgc
    35821 gagccgttcg ccagcaacgg gctgtttgcc attaccggcc caaccggtgc ggggaaaacc
    35881 accctgctgg acgccatttg tctggcgctg tatcacgaaa ctccgcgcct ttctaacgtt
    35941 tcacaatcgc aaaatgatct catgacccgc gataccgccg aatgtctggc ggaggtggag
    36001 tttgaagtga aaggtgaagc gtatcgcgcg ttctggagcc agaatcgggc gcgtaaccag
    36061 cccgacggta atttgcaggt gccacgcgta gagctggcgc gctgcgccga tggcaaaatt
    36121 ctcgccgaca aagtgaaaga taagctggaa ctgacggcga cgttaaccgg actggattac
    36181 gggcgcttca cccgttcgat gctgctttcg caggggcaat ttgctgcctt cctgaatgcc
    36241 aaacccaaag aacgcgcgga attgctcgag gagttaaccg gcactgaaat ctacggacaa
    36301 atctcgtcga tggtgtttga gcagcacaaa tcggcccgca ctgagctaga gaagctgcaa
    36361 gcgcaggcca gcggcgtcgc gttgctcacg ccggaacaag tgcaatcgct gacagcgagt
    36421 ttgcaggtac ttactgacga agaaaaacag ttacttaccg cgcagcagca agaacaacaa
    36481 tcactaaact ggttaacgcg tctggacgaa ttgcagcaag aagccagccg ccgtcagcag
    36541 gccttgcaac aggcgttagc cgaagaagaa aaagcgcaac ctcaactggc ggcgctcagt
    36601 ctggcacaac cggcacgaaa tcttcgtccg cactgggaac gcatcgcaga acacagcgcg
    36661 gcgctggcgc atactcgcca gcagattaaa gaagtaaata ctcgcttaca gagcacaatg
    36721 gcgcttcggg cgagcattcg ccaccacgcg gcgaagcagt cagcagaatt acagcagcag
    36781 caacaaagcc tgaatgcctg gttacaggaa cacgaccgct tccgtcagtg gaacaacgaa
    36841 ctggcgggtt ggcgtgcgca gttctcccaa caaaccagcg ttcgcgagca tctgcggcaa
    36901 tggcagcaac agttaaccca tgctgagcaa aaacttaatg cgcttacggc gatcacgttg
    36961 acgttaaccg ccgatgaagt tgctaccgcc ctggcgcaac atgctgagca acgcacgctg
    37021 cgtcagcgcc tggtcgcgct gcatggacag attgttcccc aacaaaaacg cctggcgcaa
    37081 ttacaggtcg ctatccagaa tgtcacgcaa gaacagacgc aacgtaacgc cgcacttaac
    37141 gaaatgcgcc agcgttataa agaaaagacg cagcaacttg ccgatgtgaa aactatttgc
    37201 gagcaggaag cgcgcatcaa aacgctggaa gctcaacgtg cacagttaca ggcgggtcag
    37261 ccttgtccac tttgtggttc caccagccac ccggcggtcg aggcgtatca ggcgctggag
    37321 cctggcgtta atcagtctcg attactggcg ctggaaaacg aagttaaaaa gctcggtgaa
    37381 gaaggtgcga cgctacgtgg gcaactggac gccttaacaa agcaacttca gcgcgatgaa
    37441 aacgaagcgc aaagcctccg acaagatgag caagcactta ctcaacaatg gcaagccgtc
    37501 acggccagcc tcaatatcac cttgcagcca caggacgata ttcaaccgtg gctggatgca
    37561 caagatgagc acgaacgcca gctgcggtta ctcagccaac gacatgaatt acaagggcag
    37621 attgccgcgc ataatcagca aattatccag tatcaacagc aaattgaaca acgccagcag
    37681 cagctcttaa cggcattggc gggttatgca ctgacattgc cacaggaaga tgaagaagag
    37741 agctggttgg cgacgcgtca gcaagaagcg cagagctggc agcatcgcca gaacgaatta
    37801 accgcgctgc aaaaccgtat gcagcagctg acgccgattc tggaaacgtt gccgcaaagt
    37861 gatgaactcc cgcacagcga agaaactgtg gtgctggaaa actggcggca ggttcatgaa
    37921 caatgtctcg cattacacag ccagcagcag acgttacagc aacaggacgt tctggcggcg
    37981 caaagtctgc aaaaagccca ggcgcagttt gacaccgcgc tacaggccag cgtctttgac
    38041 gatcagcagg cgttccttgc ggcgctaatg gatgaacaaa cactaacgca actggaacag
    38101 ctcaagcaga atctggaaaa ccagcgccgt caggcgcaaa cgctggtcac tcagacagca
    38161 gaaacgctgg cacagcatca gcaacaccga cctgacgggt tggctctcac tgtgacgatg
    38221 gagcagattc agcaagagtt agcgcaaact caccaaaagt tgcgtgaaaa caccaccagt
    38281 cagggcgaga ttcgccagca gctgaagcag gatgcagaca accgtcagca acaacaaacc
    38341 ttaatgcagc aaattgctca aatgacgcag caggttgagg actggggata tctgaattcg
    38401 ctaataggtt ccaaagaggg cgataaattc cgcaagtttg cccaggggct gacgctggat
    38461 aatttagtcc atctcgctaa tcagcaactt acccggctgc acgggcgcta tctgttacag
    38521 cgcaaagcca gcgaggcgct ggaagtcgag gttgttgata cctggcaggc agatgcggta
    38581 cgcgataccc gtaccctttc cggcggcgaa agtttcctcg tcagtctggc gctggcgctg
    38641 gcgctttcgg atctggtcag ccataaaacg cgtattgact cgctgttcct tgatgaaggt
    38701 tttggcacgc tggatagcga aacgctggat accgcccttg atgcgctgga tgccctgaat
    38761 gccagtggca aaaccatcgg tgtgattagc cacgttgaag cgatgaaaga gcgtattccg
    38821 gtgcagatca aagtgaagaa gatcaacggc ctgggctaca gcaaactgga aagtgcgttt
    38881 gcagtgaaat aactattcag caggataatg aatacagagg ggcgaattat ctcttggcct
    38941 tgctggtcgt tatcctgcaa gctatcactt tattggctac ggtgattggt agccgttctg
    39001 gtggttgtga tggtggtatg aaaaaagtca ttttatcttt ggctctgggc acgtttggtt
    39061 tggggatggc cgaatttggc attatgggcg tgctcacgga gctggcgcat aacgtaggaa
    39121 tttcgattcc tgctgccggg catatgatct cgtattatgc gctgggggtg gtggtcggtg
    39181 cgccaatcat cgcactcttt tccagccgct actcactcaa acatatctta ttgtttctgg
    39241 tggcgttgtg cgtcattggc aacgccatgt tcacgctctc ttcgtcttac ctgatgctcg
    39301 ccattggtcg gctggtatcc ggctttccgc atggcgcatt ttttggcgtc ggcgcgatcg
    39361 tgttatcaaa aattatcaaa cccggaaaag tcaccgccgc cgtggcgggg atggtttccg
    39421 ggatgacagt cgccaatttg ctgggcattc cgctgggaac gtatttaagt caggaattta
    39481 gctggcgcta caccttttta ttgatcgctg tttttaatat tgcggtgatg gcatcggtct
    39541 atttttgggt gccggatatt cgcgacgaag cgaaaggaaa gctgcgcgaa caatttcact
    39601 ttttacgcag cccggccccg tggttaattt tcgccgccac catgtttggc aacgcaggtg
    39661 tatttgcctg gttcagctac gtaaagccat acatgatgtt tatttccggt ttttcggaaa
    39721 cggcgatgac ttttattatg atgttagttg ggctagggat ggtgctgggg atggtgctgg
    39781 ggaatgtgct aagtggccgg atttcaggac gttattcacc actgcgcatt gcggcagtaa
    39841 ctgactttat tattgtgctg gcactgctga tgctcttttt cttcggcggc atgaaaacaa
    39901 cgtcgcttat ttttgcattt atttgttgcg cgggattatt tgccctttca gcaccactgc
    39961 aaatattgtt actgcaaaat gccaaaggcg gagagttatt aggtgccgca ggtgggcaaa
    40021 tagcgtttaa cctcggtagc gccgtcggcg catattgcgg tggtatgatg ctgacgctgg
    40081 ggctggcata taattacgtg gcgctgcctg ccgccctgct ttcgtttgct gcgatgtcgt
    40141 cgttgctgct gtatggtcgc tataagcgcc agcaagcggc ggatagtccg gtgctggcga
    40201 aaccactggg gtagattact gtcaccgtgc gcgttgctta ttgccggatg cggcgtaaac
    40261 gccttatccg gcctacgggc tgctcatttt gtaggcctga taagacgcgg caagcgtcgc
    40321 atcaggcatg atgcgctaat tgcctggttt ttttactctt gtggccataa ccacgcagcg
    40381 ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc acattcgccg
    40441 ccgaagacaa attgtttaat caactgcgga accgtttgat ataaacggtc tacattgctc
    40501 atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg tgccagcgat
    40561 tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc gctttcttca
    40621 accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg ataatccgtc
    40681 gcaaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca ataacaaggg
    40741 acttcctcgc gataacgcag ttcgtcttcg tccatccacg gtagcggatt gtgtccccac
    40801 tcacctgccg tgccattgcc gccgatatgc gcccgcccat tgaatgccac gcccgcgccg
    40861 catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc gccatctact
    40921 gcttctgaaa ccgccagaca gttggcgtca tttgccagcc gcacttcccg ctgcaacctc
    40981 gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc attcttcacc
    41041 acgccggtgt agggcgaaat cgaaccagga atgcccatac ctaccgttcc gcgctgccct
    41101 gttgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg ccgataatca
    41161 tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc cagtgcaatc
    41221 acttcagttt tggtgccgcc taaatcgata cctatacgca cggtactctc cttatttttt
    41281 tcaatatcaa tagcgtagag acggacaact ggattggcaa tgcaaggccg ccgacaattc
    41341 gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct gtggttcaaa
    41401 aatttaatgg tttaccgtct tagccgcgag atttcgttgc gtgcagaaga gatggaaaaa
    41461 cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa gatgggctgg
    41521 gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg tcaaattgtt
    41581 atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca ggcgctggaa
    41641 gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac cgaaaaagat
    41701 tcgctgaaag acgaagtgct gcactctctg ctaccgcgtg ctttcagccg ttttagccag
    41761 acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc cagtgccaaa
    41821 aaagcggaag atacgctggc actgctgcgt aaaagcctgg ggtcgttacc ggttgtacca
    41881 ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc cggcagcgcg
    41941 gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga agatggcgga
    42001 gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaacca cattgaagcc
    42061 ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt tgtgatgtgc
    42121 gacgatggtt cgctcaagcg tctgaagttc tgcgacgagc tgcgcgatca aaacgaagat
    42181 atcgaccgtg aagatttcgc ccagcgcttt gatgccgatt tcatcctgat gactggtgaa
    42241 ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca acgttaattg
    42301 ctgatttacc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg tacgaagcca
    42361 caccaggaat ataatcgtta gctacagcgg gcaataattc ttagcgcagc aatattatgc
    42421 gttttatgct gtaacttgct ccaggggcgt tgtgtcattg tttttcctca agccggtgac
    42481 gcgcttcact gcgaattttt cgcgttgccc cgtttttcac taaatcacgt aacagcgaaa
    42541 gatcgggttg ttcgacaaat aagagtaatg atggctcctc ttttaaccac gccgttttca
    42601 catcggcagc cagctgtgaa ttattaatag ccagcgaccg ctcctgaggc tcagtcaatg
    42661 tcgttaacaa caccggagta tgtgcgttgt gcaatgccgt ttggcgcaaa tgatcattgc
    42721 gactttgcag caatgcacac caccattcat catctgattt atcttttaac gtcgccagtt
    42781 ccgcaatagt atctggagat cttcgctgat atttatatca accttgatga attatgaata
    42841 tatcggaaat cgaaaaaatc ccagcaaagg aaatattatt atgggttaaa gagaggacat
    42901 ttcagaaaaa tgccctcccg ttcaagggga aggcgaggaa ttacagatag cggcacagat
    42961 aagaggtggg ttcggcaact tgcagatgaa actcgctgtg accgggaaca ttaaacagcg
    43021 aaccggcttc atacacttgc cagtcggtcg cgtcaggcag taacacattc agcgcgccac
    43081 tgattaccgt catctcttca ggctcagcgg tgctgaaggt gtattcacct tcaaccataa
    43141 cacccacgct ggcgcgacca gtgctgctgc tggaaaagcc gatcgatttc actttgccgg
    43201 aaaagtactc attactttga agcataaact ggccctgtct ggtagttgaa catctacttc
    43261 actatagggg ccagaggcgc tggctgtcac gcaaaattac atcagtaatt ccgcagccag
    43321 ccgtgcaatc aatacgttag agagcaagac aggaacatcg agctgttttt gcagtaaatc
    43381 gcgatgacgc tggttaaatc ccaaacaatc cagcatgatg acatctgcac cttttgccag
    43441 taattctttc ccggcatcaa tgattttttg ttccgaatca tgaatgggat tacccaatga
    43501 aaatactggc ggtttctgca aaatttgcca tttttgcgcc tgaacggtca gcaactcctc
    43561 aaccggtacg ataaccccca cctgatgatc ttcaacaata gaggaaacca gcggtggcaa
    43621 tatccgcgac ggctcaagaa agatcgtatt acgcgcggtc atactactaa tgtttgctgt
    43681 actcattaat ataatgacgt cgtaaccctg attatcgagc acttcaacca caccttgcag
    43741 gtctcgctcc actttgcgac gcgaaacatg ggccagatgg ttgtcattta ataatgtgag
    43801 aatggtgtct tcgcctgctt ctggcgcgta ctccgccatc acttcttcgc ggcttaactt
    43861 ccccagcagg ctatgatggg aaatattatc ttcgtcaatg tattctgtca ggagcggcaa
    43921 aacttcctgc ataggtacaa tgccgatggt caggatcgcc aacgacgcac tcattgttct
    43981 tccaccttac ttttatttcg tcgttaatta ccgggcaagt gttaagcacc actgctgaca
    44041 cttacttgtg ctacaaatga caaaaagcgt agcagcagac ctgcactgca taatgtaaag
    44101 attccaaatg attccagtaa tggattgtta ttgtttaata ttctaattat ttgaaaaaca
    44161 tgaattgtga aaaatgtgac gcagatccct tgtggcggca ctcagaatcc cttccgagca
    44221 gtctgatgcg gtcactcttt gtcagggtcc tcatagcgtt ttttcgcatc catcgcttcc
    44281 tgagcggtcg gatgttcact gatcaacgag tctggtttag gatgatcggc tctgagttgg
    44341 taccaggtta ccgtctgtcc tggctttcct ttttcaatcg tcactatata tgcttcacga
    44401 ggataaggcg gtttcgttgg catcgttctt ccttatttca cgggatgaac gttaagtata
    44461 ggcgctcgaa aatcaacaat tgatcgtctg tgccagagcg ctgcgaattt cagaaatcac
    44521 ctggctgggt tcgtttgttg cgtcgatgat aatatgcgca acttcgcgat atagcgcatc
    44581 gcgttcttcc agcacttcct gaacttcttc gctcagtggt tttcccgtta aggttggccg
    44641 taaatcttct tccggtgcag cttgcagtcg gttaaccagg actgatactg gcgcacacaa
    44701 ataaaccacg atcccgttat tttgcatgaa gtgacgatta aattccgtca gaataatgcc
    44761 gccgcctgta gcgataacgg tggatggcgc agttaccgct tccagcgccg ccgtttctct
    44821 ggcgcggaat cccgcccact cttccctttc gacgatctcc gcgaccgtca tattgagctg
    44881 tgattgcaac cactggtcgg tatcgacaaa ccgacggtta agcgaatcgg caagggccat
    44941 tccgaccgtt gttttaccac agccccgagg cccgatcaga aaaagaggtt gtgtcatcgt
    45001 gggttttccc caataggtcg caatgcggcg aaagccggtg tcatgagaat agcgatcata
    45061 ccatcaaact agtacaattt cgattgtaaa gaaaaaattc cacttaaagt gaaaatctca
    45121 atacatccct tactatacca ataaatattc aagaatgaag tgtaaataat aaattacatt
    45181 tagccacgac tacgttgcac ttccagccac cacttctcca gctccgcagc aaaagcctgg
    45241 cggtcacgtt gtgaaagact atctggtcct ccggtctgga tcccactggc acgtaaggta
    45301 tccataaaat cgcgcatcgt caggcgctca cgaatggtcg ctggcgtgta acgttcgccg
    45361 cgcggattaa gcgccgccgc gcctttctcg atggcttcag cagccaaagg tatatctgcg
    45421 gtgatcacca aatcgcccgc ttcacactgc cgaacaattt cgttatcagc aacgtcgaaa
    45481 cctgccgcga cgcgcagcgt acgaataaat cgcgatggcg gcacgcgtaa actctggttt
    45541 gctaccagta ccagcggcat ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt
    45601 acattgggac acgcgtcggc atccacccaa attgtcataa agtcatcctt tgttgggtaa
    45661 tcctctattg tgtcgcgctt ttgccttccg gcatagttct gtttatgctt ctgccagcga
    45721 ttatcaaaac aatgaatttc acggcaggag tgaggcaatg gaaaagaaaa tcggttttat
    45781 tggctgcggc aatatgggaa aagccattct cggcggtctg attgccagcg gtcaggtgct
    45841 tccagggcaa atctgggtat ataccccctc cccggataaa gtcaccgccc tgcatgacca
    45901 gttcggcatc aacgccgcag aatcggcgca agaagtagcg caaatcgccg acatcatttt
    45961 tgccgccgtt aaaccaggca tcatgattaa agtgcttagc gaaatcacct ccagcctgaa
    46021 taaagactct ctggtcgttt ctattgctgc aggtatcacg ctcgaccagc ttgcccgcgc
    46081 gctgggccat gaccggaaaa ttatccgcgc catgccgaac actcccgcgc tggttaatgc
    46141 cgggatgacc tccgtaacgc caaacgcgct ggtaacccca gaagataccg ctgatgtgct
    46201 gaatattttc cgctgctttg gcgaagcgga agtgattgct gagccgatga tccacccggt
    46261 ggtcggtgtg agcggttctt cgccagccta cgtctttatg tttatcgaag cgatggccga
    46321 cgccgccgtg ctgggcggga tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt
    46381 catgggttcc gcaaaaatgg tgctggaaac gggagaacat ccgggaacac tgaaagatat
    46441 ggtctgctca ccgggaggca ccaccattga ggcggtacgc gtactggaag agaaaggctt
    46501 ccgtgccgca gtgatcgaag cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa
    46561 atcctgatgg cttttgccgg acgtcaggcc gccacttcgg tgcggttacg tccggctttc
    46621 tttgctttgt agagcgccaa atctgccgat ttcaaccact cgcgatagtg actcatttgt
    46681 ggattcagcg gcgcaacccc cacactaatc cgtaaagtta cctgtggcgt attcggcaag
    46741 cgtaacgtgt ttagcccttc atgcacccgt aacatggcgg tgatggcgct ctctgctggc
    46801 gtaccggaca tgattactgc aaactcatcg ccgccaaacc gaccaatcac atcgctaccg
    46861 cgtagggtaa tttgtaactg tcgggtaagc gccacaatcg cttcatcacc cacatcatgg
    46921 ccccaggtat cgttgatgct cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg
    46981 cgattatgcc gccgacagtt atcaaattca ttgcgtaaca tagtttccca atgacgtcgg
    47041 ttatacacgc ccgtcatacc gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc
    47101 gccagtttag tcgccgtctg gtagctgacc cagccaaaca gcagaggata aatgacaata
    47161 atgggaaggg agagccacca ttccagcggt gcgctattga acgacacggt gatgcccgtc
    47221 agctcgaggg tgacaaggca ggagaccacc atcaacacca gacccgcgac aaacagacgg
    47281 gggccgcctg cccccatcaa attcagacac ataatcatca acatcgcggt ggaaggcagt
    47341 gcgtttacgc ccattacgcc tacccacatt cccgctaata ctgcatcggt ttttaagttg
    47401 taaatttccc ggctaagcgg atcgacggcc ctgctcgcta tttgccaggc taaatgcggc
    47461 cagacgaacg cccagccgac caacaacagc caccaccagc ccggcgacgg atgcgaaacc
    47521 agcgttgaag caatcggtaa gaacatgcca gccaggccaa ccgcgcgggg tagtctgacg
    47581 cgacgggcaa agcgcagccc ggaccgctga tgttcgtttt gaggagttaa aggaggttcc
    47641 tccccatgcg ccgccgcttt tttgaaaaag ttttcatcat tcattatttt tgggaacatt
    47701 cagaaacaat tttcccaaat tatagagacg gggctaaagg cagaagtatg aaatttcggg
    47761 tgagccattt tcgtagctca cccgttagca ctatcaggta tcaggcggct ttcttgaggc
    47821 aggcgctcat aaacttatta cgatcatccc ctttcagaga ttgttgcgtt gcttgattat
    47881 tacattcgcg catcttttgc tgctgtggcg tcaaactttt ttcaccaggc gcagacttgc
    47941 tgttcttcag gcaatcactc atgtaggtct tacgagcatc ccctttcaac gcctgcgccg
    48001 tcgcctgctg attacaggag gtcatacgct gttgttgcgg ggttaaggtt ctctcggcag
    48061 cgccgacggt ggttaaaaaa accagtccga aaagcaaggt aaccagtaat gttattttca
    48121 tcgcaccatc cctcttcatg ttttaaccat gagcgtttgc gcccgtgatc tgccattaag
    48181 tctggttgct aacagcaaaa aaaccacccg acagcgaaaa ttcaccgccg ggcgcggttt
    48241 tatttcagcc ccagggcggc tttcatggtg tagaagagat cggtctggtc ggtcagtcca
    48301 acgacattgg cggcatgtgg gccatacgct gcaatacgca actgactgcc ggtatgttct
    48361 tgtgaatcct cttcggagtt cccgtaactc atcaccatca ctgcgccatc tttggtattt
    48421 agcgcctggg tgaggcccgg cgctttggtg tccggcgcga caatctggct ggcgtgggcg
    48481 tgatcagcgg tgactatgac cagcgtgttg ccatcctttt tagcgaattc cagcgcacgt
    48541 tgtacggctt catcgagatc gaccgtctcg ccaatttgcc cacaaggatt cgcagcgtga
    48601 tcctgtttat cgatggatgc accttcaact tgcaggaaaa aacctttctc atttttactc
    48661 aacaattcaa tggctttgtc ggtcatctgc gccagggtcg gtacactgtc attacgttgc
    48721 ggattaggcg tacaggtaac tgcgggctta tcgatattgc cgtggtacgt tgctttcggt
    48781 cctagccagc gcactggcat attgccgtca gcaaacaggc caagcagggg tttttgctga
    48841 ttcgcttccg tcaccgcatt tagcgaggca gcatcactca ccaactgata accacgcgcc
    48901 tgtgcctgtt cacgcagcgt ttttccctgc cattcgccga cggttgccgt ttcagcaaag
    48961 gtttttgcgc cgccgccaag cgtaacatcg gcacgagcgt taagcagctg ttcggtaatc
    49021 gatccttttc cgcctttttc cagagcgtta cccggacatt tttcactggt cgcgctcgga
    49081 ccgtagcatt tgcgcgaggt cacatgcacc accagcgcgg cgggcgtggc atcctgcaac
    49141 tctgcggtag aaacgttacc ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt
    49201 gggtgatctt tttcgtgaat atcgacgccc agcgcaccgt tataggtttt gacaccagtt
    49261 gaccaggcgg ttgctgatgc agccgagtcg gtgacgtagt ccggtttacc ggttttttta
    49321 ttcagcgcat agtgagtgta ttgcccggta agcggtaatg catcgatacc tttaaaaaag
    49381 ccgcccgcac cttcggcata attgcgtgca gcagtaattt ccgagtcccc catcccatcg
    49441 ccaatcagca aaataatatt tttggcgggt ttatcgctaa gagaatcacg cagagcggcg
    49501 gtctgatccc ccgttaaacg gcgagcaccg ccgggtgcag taatatcgcc ctgagcagcc
    49561 cggttttcca gaacaggcat ttctggtgtc cgggcttttg tcacagggga aaacagtaac
    49621 ggtaagagtg ccagtgcaat agtgctttgt ttcactttat tttctccatg tacaaataca
    49681 ttaaaaataa aaacaaagcg actataagtc tcggccgtga cagctttatg acagctgttg
    49741 aaaagattaa ctttttactg acgaggatgc ttcaataact tctttacata attgcgcagc
    49801 agctccgtat cgtcgtcagg aatgctggca tcgggcttta cctcgtacag cgccccctct
    49861 acctgatcaa tcaacctctg ttggtcattt tgcgccatat tgcgaagcat tgcagtgacg
    49921 ataatctcca aagcttctac ctgcgcgcac agttctttcg actcttcttc tttttgggca
    49981 agcttaaata acaactcagc aatgagattt ttcatgtctg tatttcctta tccaaagtat
    50041 ggagaagtta a
//
LOCUS       DAZGAP010000010        48545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGAP010000010 DAZGAP010000000
VERSION     DAZGAP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.010"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..641
                     /gene="comR"
                     /locus_tag="ACHLAM_000502"
     CDS             9..641
                     /gene="comR"
                     /locus_tag="ACHLAM_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO7852090.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(703..1242)
                     /gene="ycfJ"
                     /locus_tag="ACHLAM_000503"
     CDS             complement(703..1242)
                     /gene="ycfJ"
                     /locus_tag="ACHLAM_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7852091.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1469..2773)
                     /gene="ndh"
                     /locus_tag="ACHLAM_000504"
     CDS             complement(1469..2773)
                     /gene="ndh"
                     /locus_tag="ACHLAM_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO7852092.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3173..3715)
                     /gene="ycfP"
                     /locus_tag="ACHLAM_000505"
     CDS             complement(3173..3715)
                     /gene="ycfP"
                     /locus_tag="ACHLAM_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO7852093.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3738..4763)
                     /gene="nagZ"
                     /locus_tag="ACHLAM_000506"
     CDS             complement(3738..4763)
                     /gene="nagZ"
                     /locus_tag="ACHLAM_000506"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO7852094.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4774..5597)
                     /gene="thiK"
                     /locus_tag="ACHLAM_000507"
                     /pseudo
     CDS             complement(4774..5597)
                     /gene="thiK"
                     /locus_tag="ACHLAM_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
     gene            complement(5578..6219)
                     /gene="lpoB"
                     /locus_tag="ACHLAM_000508"
     CDS             complement(5578..6219)
                     /gene="lpoB"
                     /locus_tag="ACHLAM_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO7852095.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6233..6610)
                     /gene="ycfL"
                     /locus_tag="ACHLAM_000509"
     CDS             complement(6233..6610)
                     /gene="ycfL"
                     /locus_tag="ACHLAM_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO7852096.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6613..6972)
                     /gene="hinT"
                     /locus_tag="ACHLAM_000510"
     CDS             complement(6613..6972)
                     /gene="hinT"
                     /locus_tag="ACHLAM_000510"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO7852097.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7306..9495
                     /gene="fhuE"
                     /locus_tag="ACHLAM_000511"
     CDS             7306..9495
                     /gene="fhuE"
                     /locus_tag="ACHLAM_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO7852098.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9555..10988)
                     /gene="ptsG"
                     /locus_tag="ACHLAM_000512"
     CDS             complement(9555..10988)
                     /gene="ptsG"
                     /locus_tag="ACHLAM_000512"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO7852099.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11283..12080)
                     /gene="ycfH"
                     /locus_tag="ACHLAM_000513"
     CDS             complement(11283..12080)
                     /gene="ycfH"
                     /locus_tag="ACHLAM_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7852100.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPQDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12091..13095)
                     /gene="holB"
                     /locus_tag="ACHLAM_000514"
     CDS             complement(12091..13095)
                     /gene="holB"
                     /locus_tag="ACHLAM_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO7852101.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFHGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13092..13733)
                     /gene="tmk"
                     /locus_tag="ACHLAM_000515"
     CDS             complement(13092..13733)
                     /gene="tmk"
                     /locus_tag="ACHLAM_000515"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7852102.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATLPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13723..14745)
                     /gene="yceG"
                     /locus_tag="ACHLAM_000516"
     CDS             complement(13723..14745)
                     /gene="yceG"
                     /locus_tag="ACHLAM_000516"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO7852103.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKLRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14748..15557)
                     /gene="pabC"
                     /locus_tag="ACHLAM_000517"
     CDS             complement(14748..15557)
                     /gene="pabC"
                     /locus_tag="ACHLAM_000517"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO7852104.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15677..16918)
                     /gene="fabF"
                     /locus_tag="ACHLAM_000518"
     CDS             complement(15677..16918)
                     /gene="fabF"
                     /locus_tag="ACHLAM_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO7852105.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17006..17242)
                     /gene="acpP"
                     /locus_tag="ACHLAM_000519"
     CDS             complement(17006..17242)
                     /gene="acpP"
                     /locus_tag="ACHLAM_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7852106.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17453..18187)
                     /gene="fabG"
                     /locus_tag="ACHLAM_000520"
     CDS             complement(17453..18187)
                     /gene="fabG"
                     /locus_tag="ACHLAM_000520"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO7852107.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18200..19129)
                     /gene="fabD"
                     /locus_tag="ACHLAM_000521"
     CDS             complement(18200..19129)
                     /gene="fabD"
                     /locus_tag="ACHLAM_000521"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO7852108.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19145..20098)
                     /gene="fabH"
                     /locus_tag="ACHLAM_000522"
     CDS             complement(19145..20098)
                     /gene="fabH"
                     /locus_tag="ACHLAM_000522"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO7852109.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20166..21236)
                     /gene="plsX"
                     /locus_tag="ACHLAM_000523"
     CDS             complement(20166..21236)
                     /gene="plsX"
                     /locus_tag="ACHLAM_000523"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO7852110.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21317..21490)
                     /gene="rpmF"
                     /locus_tag="ACHLAM_000524"
     CDS             complement(21317..21490)
                     /gene="rpmF"
                     /locus_tag="ACHLAM_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO7852111.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21542..22063)
                     /gene="yceD"
                     /locus_tag="ACHLAM_000525"
     CDS             complement(21542..22063)
                     /gene="yceD"
                     /locus_tag="ACHLAM_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO7852112.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22262..22846
                     /gene="yceF"
                     /locus_tag="ACHLAM_000526"
     CDS             22262..22846
                     /gene="yceF"
                     /locus_tag="ACHLAM_000526"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO7852113.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22959..23918)
                     /gene="rluC"
                     /locus_tag="ACHLAM_000527"
     CDS             complement(22959..23918)
                     /gene="rluC"
                     /locus_tag="ACHLAM_000527"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO7852114.1"
                     /translation="MKTETPSVKIVAINADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24491..27676
                     /gene="rne"
                     /locus_tag="ACHLAM_000528"
     CDS             24491..27676
                     /gene="rne"
                     /locus_tag="ACHLAM_000528"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO7852115.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            27810..28103
                     /locus_tag="ACHLAM_000529"
     CDS             27810..28103
                     /locus_tag="ACHLAM_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO7852116.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            28100..28594
                     /locus_tag="ACHLAM_000530"
     CDS             28100..28594
                     /locus_tag="ACHLAM_000530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7852117.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(28689..29642)
                     /gene="flgL"
                     /locus_tag="ACHLAM_000531"
     CDS             complement(28689..29642)
                     /gene="flgL"
                     /locus_tag="ACHLAM_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO7852118.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(29654..31297)
                     /gene="flgK"
                     /locus_tag="ACHLAM_000532"
     CDS             complement(29654..31297)
                     /gene="flgK"
                     /locus_tag="ACHLAM_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO7852119.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(31363..32304)
                     /gene="flgJ"
                     /locus_tag="ACHLAM_000533"
     CDS             complement(31363..32304)
                     /gene="flgJ"
                     /locus_tag="ACHLAM_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO7852120.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(32304..33401)
                     /gene="flgI"
                     /locus_tag="ACHLAM_000534"
     CDS             complement(32304..33401)
                     /gene="flgI"
                     /locus_tag="ACHLAM_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO7852121.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(33413..34111)
                     /gene="flgH"
                     /locus_tag="ACHLAM_000535"
     CDS             complement(33413..34111)
                     /gene="flgH"
                     /locus_tag="ACHLAM_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO7852122.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(34164..34946)
                     /gene="flgG"
                     /locus_tag="ACHLAM_000536"
     CDS             complement(34164..34946)
                     /gene="flgG"
                     /locus_tag="ACHLAM_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO7852123.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQLGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(35117..35872)
                     /gene="flgF"
                     /locus_tag="ACHLAM_000537"
     CDS             complement(35117..35872)
                     /gene="flgF"
                     /locus_tag="ACHLAM_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO7852124.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35892..37097)
                     /gene="flgE"
                     /locus_tag="ACHLAM_000538"
     CDS             complement(35892..37097)
                     /gene="flgE"
                     /locus_tag="ACHLAM_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO7852125.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(37122..37817)
                     /gene="flgD"
                     /locus_tag="ACHLAM_000539"
     CDS             complement(37122..37817)
                     /gene="flgD"
                     /locus_tag="ACHLAM_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO7852126.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTSTITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37829..38233)
                     /gene="flgC"
                     /locus_tag="ACHLAM_000540"
     CDS             complement(37829..38233)
                     /gene="flgC"
                     /locus_tag="ACHLAM_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO7852127.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(38237..38653)
                     /gene="flgB"
                     /locus_tag="ACHLAM_000541"
     CDS             complement(38237..38653)
                     /gene="flgB"
                     /locus_tag="ACHLAM_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO7852128.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38809..39468
                     /gene="flgA"
                     /locus_tag="ACHLAM_000542"
     CDS             38809..39468
                     /gene="flgA"
                     /locus_tag="ACHLAM_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO7852129.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            39544..39837
                     /gene="flgM"
                     /locus_tag="ACHLAM_000543"
     CDS             39544..39837
                     /gene="flgM"
                     /locus_tag="ACHLAM_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO7852130.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39842..40258
                     /gene="flgN"
                     /locus_tag="ACHLAM_000544"
     CDS             39842..40258
                     /gene="flgN"
                     /locus_tag="ACHLAM_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO7852131.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(40298..41833)
                     /gene="murJ"
                     /locus_tag="ACHLAM_000545"
     CDS             complement(40298..41833)
                     /gene="murJ"
                     /locus_tag="ACHLAM_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO7852132.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41943..42866)
                     /gene="yceM"
                     /locus_tag="ACHLAM_000546"
     CDS             complement(41943..42866)
                     /gene="yceM"
                     /locus_tag="ACHLAM_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7852133.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(42868..43515)
                     /gene="yceH"
                     /locus_tag="ACHLAM_000547"
     CDS             complement(42868..43515)
                     /gene="yceH"
                     /locus_tag="ACHLAM_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO7852134.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(43526..44110)
                     /gene="rimJ"
                     /locus_tag="ACHLAM_000548"
     CDS             complement(43526..44110)
                     /gene="rimJ"
                     /locus_tag="ACHLAM_000548"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7852135.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            44346..45554
                     /gene="mdtH"
                     /locus_tag="ACHLAM_000549"
     CDS             44346..45554
                     /gene="mdtH"
                     /locus_tag="ACHLAM_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO7852136.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            45618..46265
                     /gene="grxB"
                     /locus_tag="ACHLAM_000550"
     CDS             45618..46265
                     /gene="grxB"
                     /locus_tag="ACHLAM_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO7852137.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPIELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            46399..46959
                     /gene="yceB"
                     /locus_tag="ACHLAM_000551"
     CDS             46399..46959
                     /gene="yceB"
                     /locus_tag="ACHLAM_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7852138.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            47065..48111
                     /gene="pyrC"
                     /locus_tag="ACHLAM_000552"
     CDS             47065..48111
                     /gene="pyrC"
                     /locus_tag="ACHLAM_000552"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO7852139.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDTVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            48185..48430
                     /gene="dinI"
                     /locus_tag="ACHLAM_000553"
     CDS             48185..48430
                     /gene="dinI"
                     /locus_tag="ACHLAM_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO7852140.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11725 a        13119 c        12083 g        11618 t
ORIGIN      
        1 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgcggc cgcccaaaag
       61 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
      121 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
      181 cggaatttac caacaaagag gggttattcc gcgccgtact cgaccgctat atcgatcgtt
      241 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
      301 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
      361 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgctga
      421 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
      481 gcggggaaat cccgacccac tgtgacgtga cgcatctggc agaattcctt aattgtatta
      541 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
      601 cccgaacgac tttgcgttta tggcccgaac tggtgaaata aggcgcaaaa aaagcccctc
      661 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
      721 aatcagttgc ccgttgctat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
      781 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
      841 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
      901 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tagcgacatc
      961 tttaccgcga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
     1021 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttacg
     1081 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
     1141 gtattgcggg ccacgttcaa acacgttcag actggccact gccgctacgc ccaacgcagc
     1201 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaatac ctccttcttt
     1261 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
     1321 cgcgggaatg gctggaaata cttagggatt cggaggagtc tgaaggaatg cctgatgcga
     1381 cacttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gataaggcgt
     1441 ttatgcccca tccgccagta caagccgatt aatgcaactt caaacgtgga cggataacgc
     1501 ggttaatact ccccaccagc atcattaatc cagttttaaa gtaaccatgc agcgcaatct
     1561 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
     1621 agccgcgtgt caggttaccc atcaggctac cgacggtgga aaagttcgac agcgatacca
     1681 gcgaaccgtg atctttatac tgataatttt tcagcggctt accgttcatc tgcgccagaa
     1741 tgttgttcat tgcgcaggtt gccatctgat gtgcggcctg agcgcgcggc ggaacaaagc
     1801 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtatatgtct ggatcgcgag
     1861 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
     1921 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
     1981 tatattcgcc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
     2041 tcagcacgcg aacgccaagt ttcgttaact cactatgggc cgcagcagag atacgtggcg
     2101 gcaatgcagg caaaatacgt tcgcccgctt ctaccagcgt tacgttcagg gcttcgttgg
     2161 tcagaccttt ataaccgtaa ctgtgcagtt gcttgaccgc gttgtgcaat tcagcggaga
     2221 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt tactttgcca ttcgcgccca
     2281 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
     2341 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttggagg
     2401 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcaatt
     2461 cacctttttc gtcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
     2521 ttacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
     2581 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacaggt
     2641 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
     2701 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
     2761 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttatta
     2821 acatagctct taattaacaa caggttacag caatgaaaat gttcttcact attgagaata
     2881 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
     2941 ataggcgact ttaccgggcg agatcaaaaa acagagttta ttgtttatta tttgagcgaa
     3001 tttgtgagag tgatatagag tgaggggtgt atggagtgcg ttcggtaggc ctgataagag
     3061 gcgcatcagg cattgatgct cgactgtcgg atgcggcgtg gacgccttat ccgacttaca
     3121 gaattcggag cgcatcaggt accggcaagg ttacctgatg cgacgagggc atttacccga
     3181 gggttttgaa cgccttaatg cgctgcaaat gcggggagat attcttgaat ttgtgcgtct
     3241 gctcttcgtc ccagacaatc tcgtaataat gatgcagctc ttcagatgtc cgctggctgt
     3301 taagagcttc atcattacgc gacaaaatca ccaaacaacg atcgcgattc ttttcgcgga
     3361 agttggtcac acacttagtg gcaatatcgg catactcttc cgggcgatca atcttgcctt
     3421 ccatgttctc ataagggaac aaattagggt tgaagatcac ctggcggata tcgcaaagaa
     3481 aaccaatccg ttccgcccag tatccgccca gaccaacgcc gcaaatcagc ggacgctcgt
     3541 cgacgttcag ttgcaacatt ttgtccactt ctttgagcag atgctgcata tcatgtttcg
     3601 gatgccgcgt actgtagctt atcaagcgta catccgggtc aataaactgc aattgtaaga
     3661 ctttctcgtg gttacccgga ctgttagagt caaaaccgtg taaatagata atcatcgctt
     3721 cctcacataa gccagggtta gtgacctgct ttctcttcct gccagcgttc atgtaactga
     3781 ttcagacggg cgctgatcgc tttccagcga gccgagtcca tcagttcctg tcgcgaaaat
     3841 gaacctttat gatacaaacg tgtaacacgt tctgccttga tcggtgacag attatctaac
     3901 acgctgacgg cccctttacg attattgcag accaggatca tatcgcaacc cgcgtccagt
     3961 gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg ctccttccat cgataaatcg
     4021 tcagagaaaa tcacgccatc aaaacccagt tcctgacgca aaacggtttt cagccaatag
     4081 ggagaaccgc tcgccggacg cggatcgaca tcactgtaga tcacatgcgc aggcataatg
     4141 gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg acatatcttt agcgcgaatc
     4201 tccgcttgcg gacgtgggtc gcacggtgtt tctttgtgtg agtctgccgt tactgcaccg
     4261 tgtcctggga agtgtttccc ggtcgttttc attccggctt catgcatacc atcaataaac
     4321 cggctggcaa ttgccagggc tttttcggga tcggaatgat aagaacgctc gccaatcgcc
     4381 gcgctgatat gcccgacgtc cagcactggc gcaaagctga tatcaatatc catagcaatc
     4441 atttcgctgg ccatcaacca acctgcctct cgcgccagtt ttccgccttc ttccattccc
     4501 aacagcgcag caaatgattg tgccgctggc aagcgggtaa aaccttcacg aaaacgctgc
     4561 acgcgtccac cttcctgatc aaccgccacc accagatgat tgcgcgaagc tgcgcggatc
     4621 tggcgcacca gttcacgtaa ctgggcagga tcatgatagt tacgcgtaaa gagaatcagc
     4681 cctcccacca gcggatgcgc cagtatttca cgctcttccg cgtccagttc gtaaccttcg
     4741 acatccaaca ttactggacc cacactgctc tccttattgt tttattaata gctgccgcca
     4801 ggtgtcatcg gccagcctga taaattgttg atcgccggtt tgtcgccagc ggtactcaaa
     4861 ccaccctgct ttgagcatca gcagccaggg aaaccatcgc ctgacctgac gccataattg
     4921 cgccggataa atcttcgcgc gagtggcata gtcattgacc aattgccggt gctggtcagt
     4981 attttccacc cacaccgccg ccagttccag cgcgatatca ccatctccgg catactccca
     5041 gtcgatgagt tttaaccctg acgcgctatg cactaaattt ccggctggac atccatatgc
     5101 aatggactta agcgtaaagg ccgtggttcc cgcgctttgc gcagacgttt taaccttcgc
     5161 agccaaccca ctgtccgccg cgccggatcg ctttgctgcc agtacagttc cagtaacggc
     5221 aacagcgtta ttcgccagcc aaaacgcggt tgttgatgta gataatacag caagcctgcc
     5281 agttcgttgg tatccggcaa atacgttttt acctcgccgg gcagatagtc gaccaccatc
     5341 cagtcacgga gatataaatg cggcttcggt gcaatgcatg cgggtagttg tgataaagcc
     5401 cgatactggc gcaagaacgc gaaccgcggc gcatcaggat cgtgcggctg acgcacaaca
     5461 aaacgctggc cctgatgttc aatgagaaaa ctcccgccac taagcccact attaaacgtc
     5521 gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt gatgggatta
     5581 ttgctgcgaa acggcacctt tacctgacca gataatttcg cccgtctgca ccagcatcag
     5641 ctgcatttga agggtcggag cgttaacatt gccagaggcg ctggagtaca gcacgtaatg
     5701 agcgccgaca ttgcgggcaa tgcctatggc tttactacgg gtgcctaaac tgtcctgcgg
     5761 cgacaaacct aactgttgct ttgccatcga cagctgctgg gcggaaacca gggtaaattt
     5821 accgttatta gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc
     5881 gttagtacgg ttgttaacgc tatcaaccag caggacgcta cccgcagtca cgccgtcagc
     5941 cccaagcatc ttactgacca tcggctgcat tgcgccattc cagtcataat ggcgaatatg
     6001 cggcgcaggc ggtgcagttt gatcttcgtg ctcaattggg cctggctgct gcggtatcgt
     6061 cggcaccgat ggcactgtgg ggacaggctg ttgcggctcg gctggttgtt ccggcgctgg
     6121 tttcacttct tctaccggcg caggttcacg ttgccccaca cacccggcga gaaacatcgc
     6181 cagcgcggta atcaaggcgt agcgactcat tttggtcatc aagattcacc ccttacaaat
     6241 atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg
     6301 ccgaatgtgc gggaagggta acgctgcgtg gcctttccag aggatgcatc tccagccctc
     6361 tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa
     6421 gcgttgagga tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga
     6481 tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattc accggaattt
     6541 ccggatgtga acgacagccc accagcacta acaacgccag agacaccagc ccaaagcatc
     6601 cttttctcat tgttacagac ctttatgcgc cagcattggt cccagtggac ggccacccaa
     6661 caaatgcata tggatgtggt aaacctcttg tccgccatgg cggttggtgt tcatgatcag
     6721 gcgatagcca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat
     6781 gcgtcccagc gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgaggatatt
     6841 cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag
     6901 atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt
     6961 ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc
     7021 gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg
     7081 atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc
     7141 tgcgaatgcg ctgaaaagac attctctgtt tggtttacac gtcaaatatg aatgcgtata
     7201 tttctcattt gcatttacat gcaaaattat tcgcacacta aatataaccg ctcatcaccc
     7261 tcctccggca tgagcctgac gacaacataa accaagagat ttcagatgct ttcaacacaa
     7321 tttaacaggg ataatcaata tcaaaccacc tccaaaccgt cactgcttgc cgcctgcata
     7381 gcactggcac tcttaccttc cgccgctttt gctgcaccag ccactgaaga aacggtgatt
     7441 gttgagggtt cagccactac tccagttgat gacgaaaatg attacagcgt aacgtctacc
     7501 tctgcgggta ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt
     7561 agtcagcagc ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg
     7621 ctggggatca gcaaaagcca agcggattcc gatcgtgctc tttattattc ccgcggattc
     7681 cagatcgata actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg
     7741 ggcgacgcac tttctgatat ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca
     7801 ggactcatga ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg
     7861 accagtcgtg aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg
     7921 tatgtggcgg atttacaaag cccactcacc gaagatggta aaatccgcgc acgaattgtc
     7981 ggcggctacc agaataacga ctcatggctg gaccgctaca acagtgaaaa gaccttcttc
     8041 tccggcattg tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat
     8101 cagcgcattg atgttaatag cccaacctgg ggcggtttac cgcgctggaa tactgatggc
     8161 agcagcaaca gttacgatcg cgcacgcagt accgcacctg actgggcgta taacgataaa
     8221 gagatcaaca aggtctttat gaccctcaag cagcggtttg ctgatacctg gcaagcgaca
     8281 ctgaatgcca cccactccga agtcgaattt gacagtaaaa tgatgtatgt cgatgcctat
     8341 gtaaacaaag cggatggtat gctggttggg ccatacagta attatggacc tggctttgat
     8401 tatgtcggcg gtaccggttg gaacagcggc aaacgtaaag ttgatgcgct ggatttgttt
     8461 gctgacggta gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac
     8521 agcaaacaaa acaatcgtta cttcagttca tgggcctaca tcttcccgga tgaaattggc
     8581 agtttctaca actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg
     8641 caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat
     8701 ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac
     8761 agcatggaga aaaaccacac cacgccttac gctggtctgg tgtttgacat caatgacaac
     8821 tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca
     8881 ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg
     8941 atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct
     9001 cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat
     9061 gggacagtca gtaaaggtgt ggaatttgaa ctcaacggcg caattaccga caactggcag
     9121 ctgacgtttg gcgcaacgcg ctatattgca gaggataacg aaggaaacgc cgttaatcct
     9181 aatctgccac gcaccacggt taaaatgttc accagctacc ggttgcctgt catgccagag
     9241 ttgacggtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg
     9301 tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac
     9361 caggtgacga aaaacttctc gttacagggg aatgtcaata acctgttcga caaaacctac
     9421 gataccaacg tggaaggttc tattgtctac ggcgcaccgc gtaatttcag cattaccggc
     9481 acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc
     9541 caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg
     9601 gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc
     9661 agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac gcgcagacgg
     9721 gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc
     9781 ggtgccattt cgctggtacc tgtcgctttt gcgtcttcag tcgcgtcttc acgacccggc
     9841 gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatggcataa
     9901 ccgataccga cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgatgaag
     9961 tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat acagattggg
    10021 aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag
    10081 gagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata
    10141 ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca
    10201 gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca
    10261 cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca
    10321 ccaatctgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccaggcaa
    10381 cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga
    10441 gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta
    10501 aagatggcag ccaggcctga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga
    10561 tactcaggca gcttaatacg gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag
    10621 ataatccccc cgagtacgcc agtatccgcc aggtgtttag aggcgatttc ttcagcaggt
    10681 aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca
    10741 actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca
    10801 aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca
    10861 acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggc
    10921 agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc
    10981 ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgtttt cacgcgtggc
    11041 aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag
    11101 taaaataatt cagtgccgat ttgtttgacg gctatcacgt ttcaacttca gatcattgga
    11161 gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgtggtt attcaccgcc
    11221 caattttagg cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc
    11281 attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg
    11341 gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca
    11401 acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggaaccgg tgcaaggtaa
    11461 ggtgagtcag tttctaccag taaccgatcc tgggggacat aacgcgcggc atcgcgcagt
    11521 tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt
    11581 aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc
    11641 actttttctt cgcgcagaat tgccagcgta tcggcgcggg cgtcacgggt atggacgata
    11701 accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggattc ttgctgccgt
    11761 actttagttt ccggcgtgta ataataatcc aacccggttt cacccagcgc aacaacgccc
    11821 tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga
    11881 tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg catgtgtaaa
    11941 taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc
    12001 gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag
    12061 tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc
    12121 cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttaat
    12181 acctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag
    12241 gcgcgaggga gaaagatggt ttgccagttc gacgaccagg cccggcacat caacattggt
    12301 cacctgcgca gcgccatgat ggcgttttag cgcatccatc agcaacgttg ccagccagtg
    12361 taaacgcgcc ggagcttgtt catgattaag ggccgctagc agcgaatacc agtcgcccga
    12421 tggcacgcta tatgccaaag cctgacacaa tgtttcacga gcctgccagt tatctccgtg
    12481 aaacaacgcc agtgccgcgc caggcgaacc ggcacttaag cgcaatgcgg caagtaatgc
    12541 atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg
    12601 cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt ctggctcgcg
    12661 ggtagcaagg aaaaaccacg tttctgctgg tggctcttca agcgttttca gcaatgcgtt
    12721 agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctaa
    12781 gcgtgcgtgc tcattcagct tttcggtgac ctcacgtacc gcatcaatgc ccagcgtatt
    12841 tttccctttt tcgggagcca gggtgtagta atcgggatgc gtgccagcct gcatcaactg
    12901 acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg
    12961 gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagcagcgc
    13021 atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca
    13081 tggataccat ctcatgcgtc caactccttc acccagttgg tcacggtagt gcggattgca
    13141 tccatcacag cctccagcgg cagggtggca tcaatggtat gaatgctttt atcttgtgct
    13201 gccagttcca gatagcgggc gcgggtgcga ttaaaaaaat cgaaagactc ttgctcaata
    13261 cgatccagct cgccgcgtgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga
    13321 tagagcgtta agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata
    13381 tgttggtcaa taccacgtcc gccgccctga tacgcctgag tggagagatc gtggcgatcg
    13441 ccaatcaccc aagtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg
    13501 cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat
    13561 ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga
    13621 gtgaaaacca tgtcgcggat acccagttgc tcaagcgtct caaccaccac gttacgcgcg
    13681 gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc
    13741 cttaagcact ttcagataat cctgcacaga cttgttatga ctggcaagat tggtattaaa
    13801 cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc
    13861 agcagccttc agcgaatccg cccccggcgt cgctatcgca cctggcggca aaccggtaat
    13921 ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata
    13981 acgctctccc atcccgtaaa tcacggtcgg gtcggtctgc aggcgcatac caatgcgtaa
    14041 ccggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc
    14101 gataattgat gccatcgtca ccaactggtt tttatcttta taaggcagac cgtccgcacg
    14161 cccttcccag gcgctatcga ccgctttcac cattttcttg tgcgctcgct tgagtaacgc
    14221 gacatcggtg gtattggcgg tatacatcca ggtgtctggc cagaaccaac cttcaatcca
    14281 ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cgctcagcgt
    14341 gtgcttgata tagggggcct cacgcaattg cttgaggtaa tcgctcagac gcatcccttc
    14401 taccagtcgc agagggaact gtgcttcttt accgctttcc agcaatttca gcatctcgcg
    14461 cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc
    14521 gatacgcagc agccattgga aaacccgtgg gcgattgatg atcttatcgg cataaagctg
    14581 ttcaccgagc gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat
    14641 aagtaatttg ctgtcggcaa gatggcgaag cttccagacg cccacaccag cggcgatacc
    14701 cagtaccacc agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc
    14761 tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggagac atcgccacag
    14821 gcacgtacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac
    14881 gcctcctcca gcgaggcttg cacttcgaca agctgataag aggattgtgc cagcaaacgg
    14941 atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact
    15001 acgttgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg
    15061 tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcggat caatacttgc
    15121 tcaagccgat taagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg
    15181 cttagcgcca acgtcatccc ctcgttacgc aaacggtcgt aatgtgcggg ataaaccgta
    15241 acggagagaa tccgcgttgc cggtccgctg ttcaatgtgc tgtatcctcg cccgccacta
    15301 ccgcgactga tcaccacttt cagcacacca ttttgctgtt ctgctgccag cgttttcatc
    15361 tctagttcaa gctgaggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt
    15421 agtcgctgga tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta
    15481 aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttatga
    15541 ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca
    15601 ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttataag
    15661 ggtgggaaat gacaacttag atctttttaa agatcaaaga accattagtg ccaccgaagc
    15721 cgaaggagtt acacagcgtg tattccattc cgctgacctg acgtgcttcg tgcggtacga
    15781 aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat
    15841 cgcgcagcgc caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac
    15901 cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt
    15961 tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga agtaccgtga gcgttaacgt
    16021 agccaatctg actcgcttca atgcctgcat cacgcagagc atttgccatt gccagagctg
    16081 cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc
    16141 cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta
    16201 ccagcatacc ggcaccatcg cccagtacaa aaccatcacg ctctttatcc cacgggcggc
    16261 tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa
    16321 cgcccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat
    16381 acgcgataat acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga
    16441 tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca
    16501 caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg
    16561 tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc
    16621 gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga
    16681 caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct
    16741 cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat
    16801 ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg
    16861 tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt
    16921 ttgtcctcca gggagggaaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt
    16981 cgaacgaccg cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag
    17041 cctgaacggt ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt
    17101 ccagagccat taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag
    17161 aagcattgtt ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa
    17221 cgcgttcttc gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg
    17281 ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg
    17341 attttacgtt attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt
    17401 actcataacc acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca
    17461 tgtacatccc gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg
    17521 ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct
    17581 gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa
    17641 agcccggagc aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt
    17701 tactgaagcc gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca
    17761 tggtaccaac cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc
    17821 gcattaccgc ttttgacaga cggaaaacag atgaaaggtt ggtttcgata atatcgttcc
    17881 actcttcatc tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca
    17941 ggatatccac ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg
    18001 ggtcggtcac attcaacatc agacctttgc cgttggcacc taaataatca gtgatcgcct
    18061 gagcgccatt ttcactggtc gcagtgccga taactttcgc gccacgggct gcgagcgttt
    18121 cagcaattgc gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa
    18181 aattcatgat tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca
    18241 gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc actttgcccg
    18301 ggccgacttc atagagatgt tctacgcctt gcgctgccat gtattcaaca gacttcgtcc
    18361 actgaaccgg gttatacaac tgacgtacca gtgcgtcacg gatggcatca ccattggttt
    18421 cgcatttcac atcaacgtta ttcacaacag gaactgttgg tgcgttaaag gtgattttcg
    18481 ctaattctac tgccagtttg tcggctgctg gtttcatcag cgcacagtga gacggtacgc
    18541 tcactggtaa cggcagagcg cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct
    18601 caactgcttc tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag
    18661 aaacgacctg accttctgca gcttcttcac acgctttcgc aatagacgca tcatccagac
    18721 cgatgattgc cgccatagca cccgttcctt ccggtacggc ttcttgcatg aacttgccgc
    18781 gcatctcaac cagacgcacc gcatcagcga aatcaatcac accagcgcaa accagcgcgg
    18841 agtattcccc caggctgtga ccagccatca ttgccggtgc tttaccgccc tgctgctgcc
    18901 atacgcgata cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc caggttttat
    18961 tcagttcttc agccggcccc tgctgggtca gcgcccacag gtcgtagccc agcgccgcag
    19021 aagcttcagc aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc
    19081 caacggtttg agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct
    19141 tatcctagaa acgaaccagc gcggagcccc aggtgaatcc accgccaaag gcttcaagca
    19201 gaaccaactg ccccggctta atgcgcccgt cgcgtacagc ttcatccaga gcgcacggga
    19261 cagaggccgc agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca
    19321 tgccgagttt tttcgctgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc
    19381 agtccagttg agaacggtca agattattcg ccgccagcgt ctcatctacg atgtgcgcca
    19441 gttctgttac cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct
    19501 ctggattcac acggtcagca ttaggcagcg tcagcaactc accatagcta ccgtcggcat
    19561 gcagatgggt ggagatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc
    19621 catcgccaaa aataataata gtcccacgat cggttggatc gcaggtgcgc gccagtacat
    19681 cggaaccgac gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc
    19741 ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa
    19801 tgcccagcat gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg
    19861 ttgccacaac gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg
    19921 tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc
    19981 ggataccggt acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg
    20041 cgtttgtccg cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt
    20101 acgctcagtc acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg
    20161 tcctgctacc gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat
    20221 acagattcca ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc
    20281 tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg
    20341 ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc
    20401 ctcgtcaggc tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc
    20461 ccttcaccct gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt
    20521 aatgtgacat ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac
    20581 tcattggctt caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag
    20641 gcatcccgaa tactgtcgag accctttact tcttcttcac caatattgag caacgccacg
    20701 cgaggattag gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc
    20761 accaacattg tactgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc
    20821 ttttgctgat gcggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg
    20881 agtaataatt ttgccagccc cattagcgcc ccggtattac cggcactgac acaggcttgc
    20941 gctcgacctt ctttcaccag ctccagtgcc acgcgcattg aactcccacg actggcgcgg
    21001 atagcttgcg aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga
    21061 cgcgaacgtt gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg
    21121 actaaaagaa gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc
    21181 acggaagggc caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta
    21241 tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca
    21301 tcacgcagac gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag
    21361 tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg
    21421 cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt
    21481 gtacggccat ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca
    21541 attacttacg ctttaagctg gctaatacgg caaatgggtt tggcttttgc gcttcttcag
    21601 gcagttcacc aaagaccatg tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg
    21661 gaactaccgg caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac
    21721 cgaattcgtt aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg
    21781 aacgcacagg actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac
    21841 gctgacactc gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt
    21901 tatcgatagc gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg
    21961 cgacgcgctc aacctgatca ggagtataga taccctggta atcaaggcgt ttttgagccg
    22021 tacgaaccgg atcgagagtc aggggtaatt ttaccttttg catagggcgc gcatattaac
    22081 tttgtaacgt catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc
    22141 gcacacattg cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc
    22201 aaaacgaagc gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa
    22261 tatgcctaaa cttattttag cctccacctc gccctggcgt cgcgctcttc tggaaaaact
    22321 gcaaatctct tttgaatgtg cagcacccga ggtcgacgaa actccacgca gcgacgaatc
    22381 accgcgacag ttggtgcttc gactggcaca agaaaaagcg caatctctgg cgtcacgtta
    22441 tccggatcat ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg
    22501 taaaccgtta acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt
    22561 caccttctat actggactgg cgttgtttaa ttcggcgaat gggcatctac aaacagaagt
    22621 ggagcctttt gacgtccatt tccgtcacct gagcgaggcg gagattgata attacgtgcg
    22681 taaagagcat cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct
    22741 gtttgagcgt ttagaggggc gagatcctaa cacgctggtt ggtttaccgc taatcgcgct
    22801 gtgtcagatg ttacgtcggg aagggaaaaa cccgctgatg ggatagtttt ctgcgccgga
    22861 tgcggttacg tcgcatccgg caatcggtgc tggcggcctg atgcggcgta aacgccttat
    22921 caaggccaac cgcccattat caggtttata tgcttgtatt agcgcgcgtt acgcagcttt
    22981 tgcaaacaac gcttcaaacc ttcatccatc ggcgcttcga tacgcatcac ctcaccggtc
    23041 cccggatggg taaacttcaa cgctgcggcg tgcaggaaca gacgatttaa tcctgtgcct
    23101 gcttcagtga gctgtctgtc aaattcacgg tcaccgtagc gatcgtcaaa ggcaatcgga
    23161 tgacccgcat actgtgtatg cacacggatc tgatgagtac gccctgttac cgggctacaa
    23221 cgcaccaggg tggcaaaagc atagcgttct tccactttaa agcgtgtttc cgacggtttg
    23281 ccttcctgac tcacacgcac gatacgttcg ccgctttgca gaatattttt cagcaacggc
    23341 gcttgaacgc tcttcacatg cgactgccac tgaccgcgca ccagcgccag gtaatctttt
    23401 tgcatccctt tttcacgtaa ttgctcatgc agagaacgca acgccgagcg ttttttcgct
    23461 accagcaaca cacctgaagt gtcccggtcg agacgatgaa ccagttcaag gaaccgcgct
    23521 tccgggcgca acgcccgcaa accttcgata acgccgaagc ttaaaccact gccgccatgt
    23581 accgccgtac cggaaggttt attcagcacc aggatatggt catcttcata taagatgacg
    23641 tccgccagcg ccgccacctt ttgcagatgt ggcgaaaccg cctcttcttc ccgttcagca
    23701 acgcgaaccg gtgggatacg cacctcatca cccgcttcga gtttatattc aggcttaata
    23761 cgttttttgt tcacccgcac ttcgccttta cgcaaaatac ggtaaatcat actttttggt
    23821 acgcctttca attgggtacg caaaaagtta tcgatacgtt gccccgcttc gtcagcgttg
    23881 atagcaacaa tttttacgga tggagtctct gttttcatgg tgggcgattc taattagcca
    23941 acaggattcg cgccactcat ttttctatgc ttatatttag ttttgcacct tattacttca
    24001 ctacgtgatc actttattga tggttattaa accaatcacc agcaagaagt gaaaaaactg
    24061 tgagtaagcg ggtgataaat ggtaaaagtc atcttgctat aacaaggctt gcagtggaat
    24121 aatgaggccg tttccgtgtc catccttgtt aaaacaagaa attttacgga ataacccatt
    24181 ttgcccgacc gatcatccac gcagcaatgg cgtaagacgt attgatcttt caggcagtta
    24241 gcgggctgcg ggttgcagtc cttaccggta gatggaaata tttctggaga gtaataccca
    24301 gtctgtttct ttgataattg cgctgttttt ccgcatgaaa aacgggcaac cgacactctg
    24361 cgcctctttg agctgacgat aaccgtgagg ttggcgacgc gactagacac gaggccatcg
    24421 gttcacaccc ggaaaggcgt tactttgccc gcagcttagt cgtcaatgta agaataatga
    24481 gtaagttacg atgaaaagaa tgttaatcaa cgcaactcag caggaagagt tgcgcgttgc
    24541 ccttgtagat gggcagcgtc tgtatgacct ggatatcgaa agtccagggc acgagcagaa
    24601 aaaggcaaac atctacaaag gtaaaatcac ccgcattgaa ccgagtctgg aagctgcttt
    24661 tgttgattac ggcgctgaac gtcacggttt cctcccacta aaagaaattg cccgcgaata
    24721 tttccctgct aactacagtg ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg
    24781 tcaggaagtc attgttcaga tcgataaaga agagcgcggc aacaaaggcg cggcattaac
    24841 cacctttatc agtctggcgg gtagctatct ggttctgatg ccgaacaacc cgcgcgcggg
    24901 tggcatttct cgtcgtatcg aaggcgacga tcgtaccgaa ttaaaagaag cactggcaag
    24961 ccttgaactg ccggaaggca tgggacttat cgtgcgcacc gctggcgtcg gcaaatctgc
    25021 tgaggcgctg caatgggatt taagcttccg tctgaaacac tgggaagcca tcaaaaaagc
    25081 cgctgaaagc cgccctgctc cattcctgat tcatcaggag agcaacgtca tcgttcgcgc
    25141 attccgcgat tacttacgtc aggatatcgg cgaaatcctt atcgataacc cgaaagtgct
    25201 cgaactggca cgccagcata tcgctgcatt aggtcgcccg gatttcagca gcaaaatcaa
    25261 actgtacacc ggcgagatcc cgctgttcag ccactaccag attgagtcac agattgagtc
    25321 cgccttccag cgtgaagttc gtctgccatc tggtggttcc attgttatcg acagcaccga
    25381 agcgttaacc gccatcgaca tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga
    25441 aaccgcgttt aacaccaacc tcgaagctgc cgatgagatt gctcgtcagc tgcgcctgcg
    25501 tgacctcggc ggcctgattg ttatcgactt catcgacatg acgccagtac gccaccagcg
    25561 tgcggtagaa aaccgtctgc gtgaagcggt gcgtcaggac cgggcgcgta ttcaaatcag
    25621 ccatatttct cgctttggcc tgctggaaat gtcccgtcag cgtctgagcc catcactggg
    25681 tgaatccagc catcacgtct gcccgcgctg ctccggtacc ggtaccgtgc gtgacaacga
    25741 atcgttgtcg ctctctattc tgcgtctgat cgaagaagaa gcgctgaaag agaacactca
    25801 ggaagttcac gccattgttc ctgtgccaat cgcgtcttac ctgctgaatg aaaaacgttc
    25861 tgcagtgaat gcgattgaaa ctcgtcagga cggcgtgcgc tgtgtgattg tgccaaacga
    25921 tcagatggaa accccgcact accatgtgct gcgcgtgcgt aaaggggaag aaactccaac
    25981 cttaagctac atgctgccga agctgcatga agaagcgatg gcgctgccgt ctgaagaaga
    26041 gttcgctgaa cgtaagcgtc ctgaacaacc tgcgctggca acctttgcca tgccggatgt
    26101 gccgccagct ccaaccccag ctgaacctgc cgcgcctgtt gtagccccag cacctaaagc
    26161 tgcaacggca acaccagcat ctcctgcaca accagggttg ttgagccgct tcttcggcgc
    26221 actgaaagcg ctgttcagcg gtggtgaaga aaccaaaccg gccgagcaac cagcaccgaa
    26281 agcagaagcg aaaccggaac gtcaacagga tcgtcgcaag cctcgtcaga acaaccgccg
    26341 tgaccgtaat gagcgccgcg acacccgtag tgaacgtgct gaaggcaacg ataatcgcga
    26401 agaaaaccgt cgtaatcgtc gccaggcaca gcagcagact gccgagacgc gtgagagccg
    26461 tcagcaggct gaggtaacgg aaaaagcacg taccaccgac gagcagcaag cgccgcgtcg
    26521 tgaacgtagc cgccgccgta acgatgataa acgtcaggcg caacaagaag cgaaggcgct
    26581 gaatgttgaa gagcaatctg ttcaggaaac cgaacaggag gaacgtgtac gtccggttca
    26641 gccgcgtcgt aaacagcgtc agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga
    26701 agaagcggta gtcgcaccgg tggttgaaga aactgtcgct gccgaaccaa ttgttcagga
    26761 agcgccagct ccacgcacag aactggtgaa agtcccgctg ccagtcgtag cgcaaactgc
    26821 accagaacag caagaagaga acaatgctga taaccgtgac aacggtggca tgccgcgtcg
    26881 ttctcgccgc tcgcctcgtc acctgcgcgt gagtggtcag cgtcgtcgtc gctatcgtga
    26941 cgagcgttat ccaacccaat cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact
    27001 ggcctctggc aaagtctgga tccgctatcc aattgtacgt ccgcaagatg tacaggtcga
    27061 agagcagcgc gaacaggaag aagtacaagt gcagccgatg gtgactgagg tccctgtcgc
    27121 cgccgctgtc gaaccggttg ttagcgcgcc agttgttgaa gaagtggctg aagttgtaga
    27181 agcccccgtt caggttgccg aaccgcaacc ggaagtggct gaaacgacgc atcctgaagt
    27241 aattgccgcc gcggtaactg aacagccgca ggtgattacc gagtctgatg ttgccgtagc
    27301 ccaggaagtt gcagaacacg cagaaccggt ggttgaaccg caggaagaga cggctgatat
    27361 tgaagaagtt gccgaaactg ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc
    27421 tgccgcgcca gtcgtcgctg aagtcgcagc agaagttgaa acggtagccg cggttgaacc
    27481 agagatcacc gttgagcata accacgctac cgcgccaatg acgcgcgctc cggcaccgga
    27541 atatgttccg gaggcaccgc gtcacagtga ctggcagcgc cctacttttg ccttcgaagg
    27601 caaaggtgcc gcaggtggtc atacggcaac gcatcatgcc tctgccgctc ctgcgcgtcc
    27661 gcaacctgtt gagtaataat tagctcaagt aatcaagccc tggtaactgc cagggctttt
    27721 ttatttcagg ggtagcatct ttgctgtaat gacattagcg ttacatgtac tgtataatta
    27781 tcatccacgc aaagccgaag gatatattta tgccagcagc caataacatt ccagccaggc
    27841 gcgaatcttt aaacttgcgc ataaaacccg cagaacgcga tcttattgac cgggccgcta
    27901 aagcccgagg aaaaaatcgc accgaatttg tccttgaggc cgccagagcc gcagctgaag
    27961 aggcgctcat tgagcagaga attttcagga tcgattctga tgattatcag ctatttttga
    28021 atcgtctgga tcagcccccc gcgcccaatg ctgcattacg caaaaccatg caaacacccg
    28081 cgccgtggga gcaaaaaaaa tgattcaggc ccccgagcca ctcatggcaa ggcatcaatt
    28141 cacctctttt tgctcgggag tagaaacgat ggataactgg ctaaagcaga gagccctaaa
    28201 aaatcagctt gccggcgcat cacgtacatt tgttagctgt gatacgtata gcaacgtttt
    28261 ggcatattat tcgcttgctt ccagcgccgt tgaaacgtat gtcgcgacag gacgatttcg
    28321 ccgaaatatg ccagaaccga tccctgttgt agtacttggt cgtctggcaa tagataaatc
    28381 attacagggt cagggcatcg atcgtgcgat ggtcagagat gccggtctac gagtactaca
    28441 ggcggctgaa gtcattggta ttcgcggaat gttggtccat gcgttatcag accaggcccg
    28501 tgagttttat ctgcgagtgg ggtttgaacc atcgcctgtc gattctatga tactgatggc
    28561 aacattggct gatttgcagg aatgtcttaa atagttcgtt atgtctcatg caaaaaaatg
    28621 cccgccccgg cgatcccaga gcaggcagac aaaacatacc cagtttcatg atatgtttta
    28681 aagcgaaatt atttgttgag ctggaagagc gacaatccct gcatatcggt aaatgcttta
    28741 tacgacgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc attccagtcc
    28801 acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct acctaatgaa
    28861 tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacagt cagcacattg
    28921 ttcagcgagt ttttcagtcc gcggttggtt ttatccagtg ccgcagcggc ggtttcttta
    28981 tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcgctatc cagcatggca
    29041 aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttgct ggtaatactg
    29101 tcgaaaattt tgtcacccgt gtgccctatc accatcgaac gcgaagcatc gacctgttgt
    29161 ttaatacttt ctgctccgcc ttcgtatttt ccatccgcct cgctaaacgg tgcggattct
    29221 gttttataac cggcaaaaat gtagcgcccg ttaccgtcag tggtgttcgc cagattcagc
    29281 aactggtcac gaagcccctg aatatccgta gccagcgagg cccggtcatc gtcactcaag
    29341 gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcggt ggtgacctgg
    29401 ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgcgc cagcgtgtac
    29461 tggctgtttt gcgcctgtgc ctgggagaga acaacagctt gtgatgcagc aatgggatca
    29521 tctgaagggt taacgactcg cttacccgtc gacatctgtt cgccgtactt catccattct
    29581 gcctgagaat tggtgatacc acgcatgttt tgctggtaca tcatctgggt actgaaacgc
    29641 atcttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt gccgtctgca
    29701 gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg tactcctcat
    29761 cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc acattacctt
    29821 gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc actaacgagg
    29881 cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt tgcagatcga
    29941 gcagggcctg accgttgcgg ttgtcgctat cacctgtatc ttctgcgctc gccatggcga
    30001 ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt accggtttca
    30061 gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga ccatcaaatg
    30121 ccactttacc gctggcatcc ggcgtcaccg taaaagtggt attactggca aggcgggtga
    30181 cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc gcggaggcat
    30241 cagttaccgt tgcaccgatc gcaacgtcac ctttgttttt cgtgttttgc agaaccgcgg
    30301 gcttaccgat agcaaagaaa tcttcaccgg catcgccgtt agcatcaaat ccggctttgt
    30361 gttgggtgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta ttacgcgtct
    30421 ggtccagatc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta ttcagtaatt
    30481 tctccgggat ctcaatattg cctgccgtcc catcaacata agcgacagtc gtacgagaag
    30541 ggtcagcgct ggaaggaact gccgccagtt gccgagccgt acttccctga accagtgagt
    30601 aaccattggc catcgtgatg ttataagtgc caccatcctg aacgctgact tctacaccaa
    30661 caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg
    30721 cccctgcccc cacgcctgtc aggcgggaga tttgatcgtt caggctggcg atttgtttag
    30781 cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt
    30841 cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca
    30901 gcgcctggcg cgctgccgga tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga
    30961 aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg
    31021 acatctgctc atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt
    31081 tggtaataaa cgcatcatac tcacgctgca caccagaaac gtagacgcca ttgccaaccc
    31141 agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc
    31201 cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt
    31261 tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat aagccttcaa
    31321 ttacagagta ttatcggcag cgccccgccg gacttgagtt attcagaaca gattatcaat
    31381 gttcatgctg taggttttgc tcaccttgtc gcttatcgat ttcatctgct gaatcatgct
    31441 ggtgagtttg cgggcatagt gaggatcggt ggcatagccc gcgtcctgta gggcctgcgc
    31501 cccctgctcc gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gcgttaacag
    31561 cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaact tcgcttttac
    31621 tttcttcgct tcgccatttt catattcagt cgtggtgatt tcagtgactg gccctttcca
    31681 gttgccagag gctttgacgc caaacaggtt atagctcggc tcgccgtttt cacggcggat
    31741 ttgccgttgt ccccagccag attccagcgc cgcctgagcg aggatcaaat gatgtggcac
    31801 accgctttgc tggcttgcca gttgggcggg caacgagagt tgcgcgagga atgctttact
    31861 gtcacccggc agcgaatcat cgtagttacg tggtacggcc ttttgcacca gctgcgaaag
    31921 cgtctgattt tgataacgca ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt
    31981 ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct ctgccagccc
    32041 cagacctttg cccgccgtca tctgttgggc aatctgctgg tcatacatac tggtatacag
    32101 gcgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa
    32161 catcatctgc acgaacatcc cttccacctg gcgggccacc ggacggatat ttgccgccgg
    32221 atcttcgccc gctttcgcct taagttcgtt gagcgattgt gcatcccagg ccgcacttgc
    32281 cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca
    32341 ctttgcattg attgcaaaat agacatcaga tccatcggcg tagcgcccag cgcattgagc
    32401 gcacgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc
    32461 tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc aaacggtgta
    32521 tctggctggc tgacattggc ctggcgatta actgttactg agagatttcc ctgcgctacc
    32581 gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc
    32641 actactttag cgtcctgcgg ggtaacatta acctgcatat tctggatatc ggcaaggaaa
    32701 cggacctggg aactgttgcc actcggtacg cgcacctgaa tagtccgcgc atccaacgcg
    32761 gtggcgctgc catatccacg cactcggttg atggtgtcag cgatttgctg tgccatgctg
    32821 aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat
    32881 tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt cacctgaaca
    32941 ctgctaccgc cagcggaggc ccctgcgccg ccaaccagaa tattgccctg cgccagcgca
    33001 tacacctgac tgtcaacgcc cttaagcggt gtcatcaata acgtaccgcc acgcagactt
    33061 ttggcatttc ccatggaaga aaccaccacg tcaatggttt gcccctgacg tccaaacggt
    33121 ggaagtgacg ctgtcaccat aaccgcagcg acgtttttta gctgcatatt agtgcccgtc
    33181 ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc
    33241 tgagttgtct ggtcaccagt tccatccagc cccaccacca ggccatagcc aatcagtgag
    33301 ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc
    33361 gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg
    33421 gagacaggtt aagaaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc
    33481 cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg gtattgctgc
    33541 cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta ccctgattaa
    33601 tggcgatctg tttttcaccc accacatgca ggttgccgtt aaccagtacc tggtcaaccg
    33661 tcaccgtcaa cgtgccgcta aaggtattac tggcattggc cccgcctttt ccgttgaacg
    33721 tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc
    33781 gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc
    33841 ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg
    33901 gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa
    33961 tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc gccccctgta
    34021 ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa
    34081 tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg
    34141 gtgaatctgc caccggttat gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg
    34201 gtggataccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt
    34261 tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg
    34321 ttcaggcccg gcgtgctttc gttcggtgca ccggaggatt gcgtttcggt gtagaggttt
    34381 tcgccaatac tctccagtcc ggtatcattc ataaaggtgg tgagattgag ctgcccaacc
    34441 tgaaccggag ctgcctggcc ttgttgtgtt acgctgacca cgccatcacg accgatggtg
    34501 atacttagcg cattcgccgg aatggtgatc gctggctgca cctgaaaacc acctgccgtc
    34561 accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc tgatgagcca
    34621 tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttgttg
    34681 gtctgcgaca gattgccctg gctgtgtaag cgttcagtgg cgaccgggcg tacacccgtg
    34741 ccgatttgta atccggatgg taaggtggtt tgttcggaag actgagcacc tagctggcga
    34801 atggtttgat aaagcagatc ttcaaatacc gcgcgctgac gcttaaaacc gttggtactg
    34861 acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcatc caggcccgtt
    34921 ttggcgatcc ataatgaact tatcataaaa tgtcctgtag tggattagtg acaaagtgca
    34981 tctgtcttct tgccggatgc ggcgtaaacg ccttatccgg cctacaagag ctcgcgaaat
    35041 caataaattg cgattgcccc atagacccga taaacttgcg catcgggcaa cttatacttg
    35101 tcatgtatcc tttcaattaa ctcatcgaca gcagttggtt ggctcggcct gcgttatcat
    35161 cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca atcatgtcgc
    35221 tcattgccgc aacggcattg acgttactgc cttccagaac acccgacatc acacgcaagg
    35281 ttggatctgc ctgcagtacc ggcccacgcg tggtctgggt ttctgcgctt aaacgaaaaa
    35341 tgccgtcgtc accgcgctgg acttcgctgc ccgtggcttt caccagtttc agacgcccca
    35401 ctggcgcaac cgtatttgcc ggatcgcccg gattcagcgc cgagattgtg ccatcggcag
    35461 caatagtgat ttccgcccct tccggcacag caattggccc agcctcgcct atcaccggat
    35521 gtccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta cgcgtatacc
    35581 cttcgctacc gtcagcggtc tgcacggcca gccagccatc ctgctgcaac gcaacgtcca
    35641 gcgggcgcga ggtgtaatcc atcttgccgg gcgtcatgtc tgcacccggc gttgacgccg
    35701 ttaccagcgt gcgcgtaggc agtgaaagcc cttccaccgg cactgcgcgc aaggcgttta
    35761 actgcgcgcg aaaaccgggc gttgaggcat tggccagatt actggcggtt accgcctgtt
    35821 gattcaacgt ctggctggct gctcccatcg cggtataaat tgcgtgatcc attgagctat
    35881 cccgtcagcg attagcgtaa gttaaccagc gtgttgagga tctggtcctg ggttttgatg
    35941 gtctgggcgt tagactgata gttacgctga gcaacgatca tattgaccag ttctttactg
    36001 agatcgacgt tggacgcttc cagcgcaccg ttggtcaggg tgccaaagtt gcccgtcccg
    36061 gctgtcccca acagcgccac gccagaagat tgcgtcgcag accagacgtt gtcgccttcg
    36121 gatgccagac cttcgttgtt ggcaaagttc gccagtacaa tctgccccag cagttgggtt
    36181 tgttcgttgg aatagttgcc gacaaccgta ccgtcatcat tgatttgata actcaccaga
    36241 tcgcccggtt tgtagccgtt ctgggtggtt gccacaatgt tgttagcgcc ggtattttgc
    36301 tgcatggagt tgaggaagct caggctaaac gtggcgggtt ctgcaccgtt aattgcgcca
    36361 gtggaaatat tcgctgttgg atctgaggtc agaacgccat tggcattaaa caccagcgtc
    36421 attgcaggct cagctgtacc tgttggatca ctgctatcct gggtgtagac ctgccagtta
    36481 ttatccccgg tcttcacaaa gtagacgctc atgtcatgag cattaccctg actatcgaaa
    36541 acagtcaccg aacctttttt gttatagcta tccgcattgc tagcatcaaa tgcgttaaca
    36601 gagggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt ggtagttttc
    36661 gctgccatca gggtattcgg aatcgaaata ttggtcggat tcgccccttg ctgaatagtc
    36721 ggcggcgtac cggttgccgg gtaacccgtc agctgtaaac cttgcatatt caccaggtta
    36781 cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc gttgctgtct
    36841 accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc ggtgttggtg
    36901 gtcgtgccat cggtaaagtc ctgagtgata ccagcaactt ttacccccag tcccactttc
    36961 gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta ggtggcagag
    37021 ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc
    37081 gcttgagaaa aggccatgac tgactcctga aatgtgaagg cttaaattat ctgccgtact
    37141 tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgtt gttgccgcgg
    37201 atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt accaccgtta
    37261 ctggcgctaa tcgctacgtt gtaagaaccg ttcggcgcag tggtgccgtc agtcaacgta
    37321 ccgtcccagg tgaaactgtg gactccggcg gtcagttcac caatatcaat ggtacggaca
    37381 accgcaccgt ttttatcggt gatggtggac gtcactttgt ctgccgcctg ttgcagctca
    37441 acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc cgcaagaaca
    37501 gtggtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa cgactggctg
    37561 ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat cccgctgacc
    37621 gtgctgattt gagccaattg cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc
    37681 tggtttttca gctgcgccac caacaaagtc agaaaactgc tttgtaaatc tgcggcgttg
    37741 ctgcccgtga gcgaactact actggtggtg ctgacgccgg tatttgtcgg atcggtggtg
    37801 gttaccgcaa tggacatagt tttctccttt attgaccgag cgtaagggtt ttcagcatca
    37861 tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac gccgacatgg
    37921 tgttaaccat ctctccgacg acatcaacgt ttggcatttt cacgtagccc tttgcatccg
    37981 ccagcggatt acccggttca taaaccagtt tgtccggggc ctgactttct ataacatcgg
    38041 caacctttac gccgcctgtc gcagcacctg gcgcagcgtt aacctgaaat accacctgtt
    38101 ttgcccgata tggctgtcca tcgggaccgg tcacgctatc agcattcgcc agattactgg
    38161 ccgccacgtt caggcgctgg gactgagcag ttaacgccga cccggcgata tcaaaaatat
    38221 tcagcagtgc catccgttaa tttccgctct gtaaaacgtt catcatgcct ttgatttgcc
    38281 cgctcaacgc gctaaggctc atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt
    38341 cgcgatccat atcgacagta ttaccatcca gcgagggctg gtccggaata cggtattgca
    38401 gttctgcggt aggaggcgtc agcgcctgcg ccggaatgtg ttgcgttgag gtcatcgtca
    38461 gtgcaaccac gcttgttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa
    38521 aatcgatatc gcgcgcctga taaccagggg tatcggcatt ggcgatgttt gctgccagca
    38581 cttcctgacg ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga
    38641 gcttatcgag catatctcct ccgcaggtat caaaattcgg ccatcagctt aaatgccttt
    38701 acgcgcgcgt tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc
    38761 attgcggaca ggtacaattc acgttgtaga aatggctggg gggcgaaaat gctggcaata
    38821 aaacgtagcg tggcgatcat cgcgatactg ttcagtccgt taagtgcggc gagcaatctc
    38881 acatcgcaat tgcacacctt ttttagcgcc cagctcgcgg gggtaagtga tgaggttcgt
    38941 gtttctattc gtacagcgcc caatctacta ccgccgtgcg agcagccatt gctttcgatg
    39001 agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa
    39061 cgatatttac aggttaatgt gcaggccaca ggaaattatg tggttgccgc aatgcccatt
    39121 gtgcggggag gaaagctgga agctggcaat gtcaaactga aacgcggacg gctggacacc
    39181 ttgccaccgc gtacggtgct ggatatcaat caacttgttg atgccgttag cctgcgcgat
    39241 ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg
    39301 ggacaacgcg tcaacgtgat cgccagcggt gatgggttta gcgccaacgc agaaggtcag
    39361 gcgctgaaca atgctgccgt cgcacagaat gcgcgggtgc gcatggtatc gggacaagtg
    39421 gtcagcggcg ttgttgatgc agatgggaat attcttataa acctgtaagc tgttaaagat
    39481 tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa
    39541 accatgagta ttgatcgcac ttcgcctctg aagcccgtaa gcaccgttca accgcgcgaa
    39601 accactgacg cgccggtaac gaacacccgg gcggcaaaaa caaccgcctc caccagcacc
    39661 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    39721 cttgaacgtg tcgaagcgtt aaaactagcg attcgtaacg gtgaactaaa aatggacacc
    39781 ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag taactgatcg
    39841 tatgacgcgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    39901 ggtaatggat caagagcagc aacatctctc tatggggcag atcaacggca gccagttgca
    39961 atggattaca gaacaaaaaa gctctctgct ggcgacgctg gattacctcg aacagttacg
    40021 caggaaagaa cccaatgcag caaatagcgt tgatattagt caacgctggc aggaaattac
    40081 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    40141 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    40201 tggagcgaac ggtcagacct caacaaccca tcgcggcagt aaaaagattt cgatctgaag
    40261 attatctccg gcctgcactg caggccggaa tgccttttta caccgtccgg cgggcaaact
    40321 ctttaacttt gaagcccagt accgccagtg ctgcaaagta cgcggcaatc cccgccagca
    40381 cgaccgccat taaacgcagt aaacgccagg gcatggtacc caatgaccac tccggcatga
    40441 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    40501 gaaacgccat ccagccgggt tgcggggtaa agattttctg cttacgcaac tgccagtaaa
    40561 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg gcatgtttca
    40621 acggaccgat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    40681 tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact ttcactacaa
    40741 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    40801 aggcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacctgaa agaatgccca
    40861 acgcaaccgc actcggcagc gccaacagga aacaaagacg cagcccccag tccatcaaac
    40921 ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg
    40981 tgccaagcgc cacgcccagt acaccggacg gaaactccat taagcggtcg gcgtaataca
    41041 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    41101 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc
    41161 cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    41221 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    41281 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgata ttaagcagtg
    41341 ttggagcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    41401 acgccaggga gatcagcaag atatagggaa aggttatctt tagtagctgg ctggtcaggg
    41461 caaatttgtc ggcagtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcag
    41521 cgagcatgcc agcgaccgtc acaaccgcca gcgcgagtgt cagcaggcca gaaacataag
    41581 agacaaagac ccgcgtggcg tcttcaccct gcttgctttt atattctgcc agaatcggta
    41641 caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg caacaagtta ggaagtttaa
    41701 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    41761 cacgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    41821 ttaataaatt catcggtgtt ctaatccaga cccacgggca aaacgcctgc aaagcaggcg
    41881 ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag atgttacagg
    41941 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc ccgcactgcc
    42001 tgttcgccgg cggtttgcgg aactgtctga ttttgcacgc attcaatgaa gtgccgcgca
    42061 cagccgacaa atccacgttg ctcaagcgta ctctgccaac caggaatcgg tttatgcact
    42121 acgccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcaccg
    42181 tcagtcacgg cctgcacggt ttcacgctgg cttccggcac ggcgatgcat acaggtggtg
    42241 atctgcaaag gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta
    42301 gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc
    42361 accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacgctattg
    42421 ctacgatgtt tatccattct tagcgaggct gcggtggcga gttgcgtttt taactctccg
    42481 tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc
    42541 agttccacca accgttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca
    42601 tgtacccccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggaatgc
    42661 acaaaaaccg catcgcaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc
    42721 cagctttcac aaattggcag ggctttcgcg cgcgtaggcg accaggctcc ttgtaacatc
    42781 cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc gcctaatccc
    42841 actacgccga tacgtaattt tttcacttta atctcccaga tgggcaagca acgaatcaag
    42901 acgctgtttc agttctgcca cttcgatttc cagggcttcg acgcgagcct gtaaatcacc
    42961 gtcaaccgcg ttcgacatat ccatcaccgc cggctgatct tcaacctcac cactgaaaag
    43021 atgcatgtag cggctttcac gtttacccgg ttcgcgggcc agacgcacca caaaaggacc
    43081 atcttcgcga tttgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc
    43141 atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac gcaataacaa
    43201 cgtggtgatt aacgccactt ctgcggcgct cagtttcaga tcgccaaatt ctgaattaca
    43261 aaaacgttgc tcatatttgg tgacccgatt accaaaaccg ctcactgtgc gtagataatg
    43321 acgtttgacc agattatcca gctgttcctg cacttcggat tcactcagat tcatgaccgg
    43381 ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg
    43441 ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc acgcgagctt caagggcagt
    43501 aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg
    43561 ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt
    43621 ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat
    43681 tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg
    43741 cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg
    43801 catgaaaaga gccacgaaca acattggaaa aattggcaac gccaataatc tctttttcgt
    43861 ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc
    43921 ctagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg
    43981 gcttgaggaa atggcgattc tctgcgtaat aatccgccag acgccaggca tcacgatcat
    44041 gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat
    44101 agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact
    44161 atacctgcgc aaccagaccc tgtgaaaagt ccagaatgcc attttctgca ttattcacat
    44221 atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt ccccgaagtg
    44281 atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag
    44341 gggaaatgtc ccgcgtgtcg caggccagga acctgggtaa atatttcctg ctcatcgata
    44401 atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg
    44461 atcaaatggg ctgggccgcc gtcatggtcg gtattgccct cggcctacgc cagtttattc
    44521 agcaaggtct gggtattttc ggcggagcaa ttgccgaccg ctttggtgcc aaaccgatga
    44581 ttgttaccgg tatgctgatg cgtgccgccg gattcgccac aatgggtatc gcccacgaac
    44641 cgtggctact gtggttttca tgcctgcttt cgggactcgg cggcacgttg tttgatcctc
    44701 cgcgttcggc gctggtggtg aaattaatcc gtccacagca gcgtggtcgt tttttctcgc
    44761 tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg agctggctgt
    44821 tgcaatacga ctttcgcctg gtctgcgcca caggggcagt tttgtttgtg ctgtgtgcag
    44881 cgtttaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg cccgttcgcg
    44941 aaggcatgac ccgcgtgatg cgcgacaagc gttttgtcac ctatgtactg acgctggcgg
    45001 gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg
    45061 cgggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt
    45121 tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac cggttgatgg
    45181 ctgggctatt gataatgtca ttaagcatga tgccggtggg catggtcagc ggtctgcaac
    45241 aacttttcac cctgatttgt ctgttttata tcgggtcaat cattgccgag cctgcgcgtg
    45301 aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc
    45361 gtctgggtct ggcgattggc ggcgctattg gttacatcgg tggcggctgg ctgtttgacc
    45421 tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggcatt attggcatct
    45481 tcactttcct tgcgctgggt tggcagttta gccagaaacg cgccgcgcgt cgtttgctgg
    45541 aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt gaactgacag
    45601 ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag
    45661 cccgcatgat tttcggcctg aaaaatatcc ccatcgaatt acatgttctg ctcaacgacg
    45721 acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg
    45781 acagccgcta tatgccagaa agcatggaca tcgttcacta tgtcgataaa ctcgacggca
    45841 aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg
    45901 gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta
    45961 ctcctgccgc gcgcaaatat ttcgtcgaca agaaagaggc tagcgcgggt aattttgccg
    46021 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgattta cgtgcgctgg
    46081 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
    46141 tatttccgct cctgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg
    46201 ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
    46261 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt
    46321 ctgctggcgc gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat
    46381 ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc
    46441 ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag
    46501 tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc
    46561 catattgttc tgacaaacct gaccagccaa attggtcgcg aagagccgaa taaggttacc
    46621 ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagccagaa agcgaccatg
    46681 aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggcgctat cttcctgaaa
    46741 gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg
    46801 ttgcttccct atttaaacca ggcattacgc aattacttta accagcaacc tgcttacgtc
    46861 ctgcgcgaag atggcagcca gggcgaagca atggcgaaga aactggcgaa aggcattgaa
    46921 gtgaagccag gcgaaattgt cattccgttt actgattaat cacgagggcg cattcgcgcc
    46981 ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatc
    47041 ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgc
    47101 ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat
    47161 accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc cgtgaccacc
    47221 gttgaggctg ccgtggctta tcgccagcgt attcttgaca ccgtacctgc cgggcacgat
    47281 ttcaccccgc tgatgacctg ttatttgaca gattcactag accctaatga gcttgagcgt
    47341 ggatttaacg aaggcgtgtt caccgctgcc aaactttacc cggcaaacgc aaccactaac
    47401 tccagtcacg gcgtgacgtc agttgacgca atcatgccgg tacttgagcg catggaaaaa
    47461 atcggtatgc cgttactggt gcatggtgaa gtgacacatg cagatatcga catttttgat
    47521 cgtgaagcgc gctttattga aagcgtgatg gaaccgctac gccagcgtct gactgcgctg
    47581 aaagtcgttt ttgagcacat caccaccaaa gacgctgccg actatgtccg tgacggaaat
    47641 gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg
    47701 gttggtggcg tgcgtccgca cctgtattgt ctacccatcc tcaaacgcaa tatccaccaa
    47761 caggcattgc gtgaactggt cgccagcggt tttaatcgcg tattcctcgg tacggattct
    47821 gcgccgcatg cacgtcatcg caaagagagc agctgcggct gcgcgggctg cttcaacgcc
    47881 ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt
    47941 gaagcattct gttctgtaaa cggcccacag ttctatggct tgccggtcaa cgacacattc
    48001 atcgaactgg tccgcaaaga gcaacaggtt gctgaaagca tcgcattgac tgatgacacg
    48061 ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag
    48121 cttcctgttg tcattaggtt attttaactg tataaataac cagtatattc aacagggggc
    48181 tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg gggctattga
    48241 cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata atgaaggcca
    48301 cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcga caaaagaaga
    48361 taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg actggtttgt
    48421 cagcgaataa tatgcagtga tttttgtttt tgccgggtcg ccccggcttt tttattttac
    48481 ttgcgaattg tgagtacaaa atttctacac ttcctcaaaa taattgcaca atatgctaaa
    48541 aaaat
//
LOCUS       DAZGAP010000011        45011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGAP010000011 DAZGAP010000000
VERSION     DAZGAP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.011"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(94..3243)
                     /gene="acrB"
                     /locus_tag="ACHLAM_000554"
     CDS             complement(94..3243)
                     /gene="acrB"
                     /locus_tag="ACHLAM_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO7852141.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="ACHLAM_000555"
     CDS             complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="ACHLAM_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO7852142.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4601..5248
                     /gene="acrR"
                     /locus_tag="ACHLAM_000556"
     CDS             4601..5248
                     /gene="acrR"
                     /locus_tag="ACHLAM_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO7852143.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5376..8738
                     /gene="mscK"
                     /locus_tag="ACHLAM_000557"
     CDS             5376..8738
                     /gene="mscK"
                     /locus_tag="ACHLAM_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO7852144.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLRDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8799..8897
                     /locus_tag="ACHLAM_000558"
                     /pseudo
     CDS             8799..8897
                     /locus_tag="ACHLAM_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="ACHLAM_000559"
     CDS             complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="ACHLAM_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO7852145.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9125..9652)
                     /gene="priC"
                     /locus_tag="ACHLAM_000560"
     CDS             complement(9125..9652)
                     /gene="priC"
                     /locus_tag="ACHLAM_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO7852146.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9722..10099
                     /gene="ybaN"
                     /locus_tag="ACHLAM_000561"
     CDS             9722..10099
                     /gene="ybaN"
                     /locus_tag="ACHLAM_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO7852147.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10252..10803
                     /gene="apt"
                     /locus_tag="ACHLAM_000562"
     CDS             10252..10803
                     /gene="apt"
                     /locus_tag="ACHLAM_000562"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO7852148.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10932..12863
                     /gene="dnaX"
                     /locus_tag="ACHLAM_000563"
     CDS             10932..12863
                     /gene="dnaX"
                     /locus_tag="ACHLAM_000563"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO7852149.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNSVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12196..12260
                     /gene="dnaX"
                     /locus_tag="ACHLAM_000563"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12916..13245
                     /gene="ybaB"
                     /locus_tag="ACHLAM_000564"
     CDS             12916..13245
                     /gene="ybaB"
                     /locus_tag="ACHLAM_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO7852150.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13245..13850
                     /gene="recR"
                     /locus_tag="ACHLAM_000565"
     CDS             13245..13850
                     /gene="recR"
                     /locus_tag="ACHLAM_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO7852151.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13960..15834
                     /gene="htpG"
                     /locus_tag="ACHLAM_000566"
     CDS             13960..15834
                     /gene="htpG"
                     /locus_tag="ACHLAM_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO7852152.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16015..16659
                     /gene="adk"
                     /locus_tag="ACHLAM_000567"
     CDS             16015..16659
                     /gene="adk"
                     /locus_tag="ACHLAM_000567"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO7852153.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            16791..17753
                     /gene="hemH"
                     /locus_tag="ACHLAM_000568"
     CDS             16791..17753
                     /gene="hemH"
                     /locus_tag="ACHLAM_000568"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO7852154.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17750..18709)
                     /gene="aes"
                     /locus_tag="ACHLAM_000569"
     CDS             complement(17750..18709)
                     /gene="aes"
                     /locus_tag="ACHLAM_000569"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO7852155.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FTGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18861..20165
                     /gene="gsk"
                     /locus_tag="ACHLAM_000570"
     CDS             18861..20165
                     /gene="gsk"
                     /locus_tag="ACHLAM_000570"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO7852156.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20249..21925)
                     /gene="ybaL"
                     /locus_tag="ACHLAM_000571"
     CDS             complement(20249..21925)
                     /gene="ybaL"
                     /locus_tag="ACHLAM_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO7852157.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22163..23383)
                     /gene="fsr"
                     /locus_tag="ACHLAM_000572"
     CDS             complement(22163..23383)
                     /gene="fsr"
                     /locus_tag="ACHLAM_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO7852158.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23601..25253
                     /gene="ushA"
                     /locus_tag="ACHLAM_000573"
     CDS             23601..25253
                     /gene="ushA"
                     /locus_tag="ACHLAM_000573"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO7852159.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLIMAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNNLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKAGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25290..25769)
                     /gene="ybaK"
                     /locus_tag="ACHLAM_000574"
     CDS             complement(25290..25769)
                     /gene="ybaK"
                     /locus_tag="ACHLAM_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO7852160.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25973..26767)
                     /gene="ybaP"
                     /locus_tag="ACHLAM_000575"
     CDS             complement(25973..26767)
                     /gene="ybaP"
                     /locus_tag="ACHLAM_000575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO7852161.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEKRISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26900..27202
                     /locus_tag="ACHLAM_000576"
     CDS             26900..27202
                     /locus_tag="ACHLAM_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7852162.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            27238..27579
                     /gene="ybaQ"
                     /locus_tag="ACHLAM_000577"
     CDS             27238..27579
                     /gene="ybaQ"
                     /locus_tag="ACHLAM_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7852163.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27637..30141)
                     /gene="copA"
                     /locus_tag="ACHLAM_000578"
     CDS             complement(27637..30141)
                     /gene="copA"
                     /locus_tag="ACHLAM_000578"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO7852164.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30404..31336
                     /gene="glsA"
                     /locus_tag="ACHLAM_000579"
     CDS             30404..31336
                     /gene="glsA"
                     /locus_tag="ACHLAM_000579"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO7852165.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31339..32631
                     /gene="ybaT"
                     /locus_tag="ACHLAM_000580"
     CDS             31339..32631
                     /gene="ybaT"
                     /locus_tag="ACHLAM_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7852166.1"
                     /translation="MMNTEGNNDNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            32662..32759
                     /locus_tag="ACHLAM_000581"
                     /pseudo
     CDS             32662..32759
                     /locus_tag="ACHLAM_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32756..33163
                     /gene="cueR"
                     /locus_tag="ACHLAM_000582"
     CDS             32756..33163
                     /gene="cueR"
                     /locus_tag="ACHLAM_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO7852167.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33164..33894)
                     /locus_tag="ACHLAM_000583"
                     /pseudo
     CDS             complement(33164..33894)
                     /locus_tag="ACHLAM_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33966..34172)
                     /locus_tag="ACHLAM_000584"
     CDS             complement(33966..34172)
                     /locus_tag="ACHLAM_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852168.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(34169..34591)
                     /locus_tag="ACHLAM_000585"
     CDS             complement(34169..34591)
                     /locus_tag="ACHLAM_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852169.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(34677..35693)
                     /locus_tag="ACHLAM_000586"
     CDS             complement(34677..35693)
                     /locus_tag="ACHLAM_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7852170.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     TQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHYSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            36060..36422
                     /locus_tag="ACHLAM_000587"
     CDS             36060..36422
                     /locus_tag="ACHLAM_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852171.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(36525..36983)
                     /gene="ybbJ"
                     /locus_tag="ACHLAM_000588"
     CDS             complement(36525..36983)
                     /gene="ybbJ"
                     /locus_tag="ACHLAM_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO7852172.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVIIGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(36980..37897)
                     /gene="qmcA"
                     /locus_tag="ACHLAM_000589"
     CDS             complement(36980..37897)
                     /gene="qmcA"
                     /locus_tag="ACHLAM_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO7852173.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            38043..38720
                     /gene="fetA"
                     /locus_tag="ACHLAM_000590"
                     /pseudo
     CDS             38043..38720
                     /gene="fetA"
                     /locus_tag="ACHLAM_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
     gene            38707..39486
                     /gene="fetB"
                     /locus_tag="ACHLAM_000591"
     CDS             38707..39486
                     /gene="fetB"
                     /locus_tag="ACHLAM_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO7852174.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(39549..40403)
                     /gene="cnoX"
                     /locus_tag="ACHLAM_000592"
     CDS             complement(39549..40403)
                     /gene="cnoX"
                     /locus_tag="ACHLAM_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO7852175.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(40464..41273)
                     /gene="ybbO"
                     /locus_tag="ACHLAM_000593"
     CDS             complement(40464..41273)
                     /gene="ybbO"
                     /locus_tag="ACHLAM_000593"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7852176.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(41263..41886)
                     /gene="tesA"
                     /locus_tag="ACHLAM_000594"
     CDS             complement(41263..41886)
                     /gene="tesA"
                     /locus_tag="ACHLAM_000594"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO7852177.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            41857..42543
                     /gene="ybbA"
                     /locus_tag="ACHLAM_000595"
     CDS             41857..42543
                     /gene="ybbA"
                     /locus_tag="ACHLAM_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO7852178.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            42540..44954
                     /gene="ybbP"
                     /locus_tag="ACHLAM_000596"
     CDS             42540..44954
                     /gene="ybbP"
                     /locus_tag="ACHLAM_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO7852179.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT        10832 a        11687 c        11895 g        10597 t
ORIGIN      
        1 gagatcctga gttggtggtt caatgactcc ttaatgttcg tcggttatgc ataaaaaagg
       61 ccgcttgcgc ggccttagtg attacacgtt gtatcaatga tgatcgacag tatggctgtg
      121 ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc accacaaaga ataccggaac
      181 gaagaagatt gccagtaccg ttgcggtcac catcccgccc attacaccgg tacctactgc
      241 gttctgcgcg ccggaaccag caccagtact gataaccagc ggcataacgc cgaggataaa
      301 cgccaacgag gtcatcagga ttgggcgtaa acgcatccgc accgcatcaa gcgtcgcttc
      361 aatcagacct ttaccttctt tatccatcaa gtctttggcg aattcgacga taagtatcgc
      421 gttcttcgcc gacaaaccaa tggttgtgag caggcctacc tggaagtaaa cgtcattggt
      481 caggccacgg aaggtggcag ccagcaacgc accgataacc cccagcggaa cgaccagcat
      541 aacggagaac ggaatcgacc agctctcgta cagcgcagcc agacacagga acacgacaat
      601 caacgaaatc gcgtacagtg aaggcgcctg gttgccggag agacgttcct gataggacat
      661 tcccgtccag tcatagccaa caccggtagg cagtttgctc gccagttgtt ccatcagctc
      721 cattgcttca ccggtacttt tacccggtgc cgcctggcct aagatttcca tagatggcag
      781 gccgttgtaa cgttccagac gcggcgaacc gtactcccaa cgagaagagg agaacgccga
      841 gaacggcacc atctgaccat cagcagcacg aacataccag tcgccgatat catccggcag
      901 catacggtat ttcgcttctg acatgacgta aactttcttc acacgaccgc ggtcgataaa
      961 gtcgttcaca tagctaccgc cccatgcagc gcccagagtg gtgttaatgt cgttgataga
     1021 aacacccagc gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg gggtatcttc
     1081 cagaccgttt ggacgtacgc tggtcaacat atcagggtgc ttcgctgctt ctgcaagcaa
     1141 ctggttacgc gcctgagtca gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa
     1201 gtcaaagccg gttgcggtac ccagttccac gattgcgggc aggttaaagg cgaaaaccat
     1261 cgcatctttg atttgcgaga aagcacgtgt tgcacgcatg gtaatcgctt caactttgtt
     1321 ttcttcgccc ggacgatcgg cccagtcctt caaggaaacg aacgcaatac ctgtattctg
     1381 accacgtccc gcaaagccga agccgttaac ggcgaacacc gactcaacgt tgttcttttc
     1441 tttggtcaga tagtaatgcg ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc
     1501 acctgctggc agctgaacca tggtcataaa tacgccctgg tcttcatctg gcaagaagga
     1561 gcttggcaga cgcacgaaca gataggccat gccgaccacg atgatcagat acagcaccag
     1621 gtaacgccct gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt
     1681 ctcgaacatg cggttaaacc agccgaagaa gcctttttta ccttccccgt gatcgccttt
     1741 ggcaatcggt ttcagcatgg tggcacaaag agccggagtc aggatcaacg ccaccagtac
     1801 cgacagcgcc attgctgaaa caatggtaat agagaactga cgatagatag caccagtaga
     1861 accgccaaag aaggccatcg gtacgaatac cgccgacagt accatcgcga taccgaccag
     1921 agcgccctga atctgcccca tcgacttacg ggtagcttct tttggcggca aaccttcttc
     1981 cgccataaca cgctcaacgt tttctaccac aacgatggcg tcatccacca acaggccgat
     2041 ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag aagccaaagg cggcaaggac
     2101 ggcaaaggtc cccagcaata ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa
     2161 gttctgcagg aacagataca taaccaggaa cacgaggatg atcgcttcga ctagcgtttt
     2221 aaccacttcg tgaatcgaga ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt
     2281 cagacccgac gggaagaacg gttccatctt cgccagttca gcacggattg ccgcagcggt
     2341 atccagcgcg tttgcaccgg tcgccagctt gatccccaga ccggaagccg gttggccgtt
     2401 aaactctgcg atgatgtcgt agttctcacc acccagctca attttcgcca catcacgcag
     2461 cagcacgcgg gaaccatcct gattcacttt cagcaggatt ttgccgaact cttcagtaga
     2521 ggtcagacgc gtctgagcaa taatagaggc gttaagctgt tggcctttca ccggcggcgt
     2581 accaccgagc tgacccgccg caacctgggc gttctgcgct ttgatggcgg taatgacatc
     2641 aaccggcgtt agctggaatt tgttcagttc attcgggttc atccagatac gcatcgcata
     2701 ctgtgaaccg aacaactgaa cgtcacccac gcccgacgta cggctgatgg catctttcat
     2761 attcgctgcc acgtagtcag agatatcctc ctgcgtcatg gtgccatcgg tgttgataac
     2821 gccgacaacc atcaggaagc tgctggatga tttctcaacg ctcacccctt gctgctgtac
     2881 ttcttgcggc agcaacggca tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat
     2941 atccgcatca gtaccagact caaaggtcag ggtgatctgc acggtacccg tggagtcact
     3001 gttagaggac atgtacatca ggttatcgat accgttcata ttctgttcga taacctgtgt
     3061 caccgtgtcc tgcactgttt tcgcatcagc gccagggtag gaggcggaga tcgttactgc
     3121 cggcggtgca atcgtaggat attgcgccac cggcagtttg aggatcgcca gcccccctgc
     3181 caacatgatg ataatggcga tcacccacgc aaaaatcggg cgatcgataa agaaattagg
     3241 catgtcttaa cggctcctgt ttaagttaag acttggactg ttcaggctga gcaccgcttg
     3301 cggcttgctg gttattatca gcggtaactt cttgtgcttt tacctggaca ccaggacgca
     3361 ctttctgcag cccacttatt actacgcgat cgcctgcttt cagaccttct gtcaccagcc
     3421 acttatcgcc gatagcctgg cttgcaacga tcggacgggt ttccactttg tcatccgcgc
     3481 caaccaccag tacggtggca tcgccacgcg gcgtacgggt tacgccctgt tgcgggacta
     3541 aaatagcgtt tggattaagc ccttcttcca gacgtgcccg cacgaacata cccggcagca
     3601 gagtgtgatc cgggttcggg aagatagcgc gtagggtgat agacccggtg gtctgatcaa
     3661 cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa cttaatgccg tcactggtga
     3721 tcagcgacac tttggctttg ccgttttctt gtttcaacgt gccattcgcc agttcctgtt
     3781 tcaggcgcag gaagtcgttg ctggactggg tcacatcaac gtagatcgga tcaagttgct
     3841 gcacggttgc cagcgcagtc gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg
     3901 acttaccaat gcgaccgcta atcggcgagg tgactttggt gtaagccaga ttgatccgcg
     3961 cagtttcaac ggcagctttc gccgcagtga ccgcagcatt cgcctgttgc gcatcagcca
     4021 gagcctgatc gtactcttgt ttactgatgt actgagtacc gagcagtttc tgataacgat
     4081 tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt cgccaaatca cctttcgcac
     4141 tgtcgtatgc cgcctgatag gtcgcaggat caatctgata gagagagaca cctgcttcga
     4201 tgtcgctacc ttctttgaaa ttacgcttca ggataatccc gctaacttga ggacgaactt
     4261 ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc agaggttcag
     4321 ttttgactgt tactacgcca acggcgggca tctgctggcc accttgttgg gcctgtttgt
     4381 cgtcacatcc tgttagggct aagctgcctg agagcatcag aacgaccgcc agaggcgtaa
     4441 accctctgtt tttgttcata tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc
     4501 aaaagttaat aaacccattg ctgcgtttat attatcgtcg tgctatggta catacattca
     4561 caaatgtatg taaatctaac gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca
     4621 agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt tctcacagca
     4681 gggggtatca tccacctcgc tgggcgagat tgcaaaagca gctggcgtta cgcgcggtgc
     4741 aatctactgg cattttaaag acaagtcgga tttgttcagt gagatctggg aactgtcaga
     4801 atccaatatt ggtgaactcg agcttgagta tcaggcaaaa ttccctggcg atccactctc
     4861 agtattaaga gagatattaa ttcatgttct tgaatccacg gtgacagaag aacggcgtcg
     4921 attattgatg gagattatat tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca
     4981 acaggcacaa cgtaatctct gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca
     5041 ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg cgtcgggcag caattattat
     5101 gcgcggctat atttccggcc tgatggaaaa ctggctcttt gccccgcaat cttttgatct
     5161 taaaaaagaa gcccgcgatt acgtcgccat cttactggag atgtatctcc tgtgccccac
     5221 gcttcgtaat cctgccacta acgaataacc ttgaatctga ctccaggatt tttcctggac
     5281 attttcgtcg ttgctattct ggttcactgc gtcgtgatat tcttgcggtt tgactttttc
     5341 aggtcgttct tcaggttcag aaaccttcat tcatcatgac tatgttccag tattacaaac
     5401 gatcacggca ttttgttttt tcagcattta ttgcttttgt ttttgtcttg ttatgccaga
     5461 acacggcgtt tgcgcgggcg tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg
     5521 cacaacttga ctcactgaat aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc
     5581 aggatctgac agatacatta gctaccctcg ataaaatcga tcgcgtaaaa gaagagacag
     5641 ttcagctacg gcaaaaggtt gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt
     5701 taactgcact tagcgatgtc gataatgacg aagaaactcg caaaattctg agtacgcttt
     5761 cgttgcgcca gctggaaact cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa
     5821 acgatctggc gtcttataac agccagctgg tttcgttaca gacgcagccc gaacgcgtgc
     5881 aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga
     5941 ctgatgtcgg cgagactgcc ttacgtccca gccagaaagt gttaatgcag gctcagcagg
     6001 ctttgctgaa tgccgagatt gaccagcagc gtaaaagcct ggaagggaac acagtcttac
     6061 aggatacctt gcaaaagcaa cgggattacg tgacggcgaa cagcgcccgt ctggagcacc
     6121 agttgcagct gttgcaagaa gcagtaaaca gcaagcgctt gactttaacc gaaaaaacgg
     6181 cgcaggaagc cgtctccccg gatgaagccg cgcgtattca ggctaatccg ctggtgaagc
     6241 aggaactgga aattaaccag cagttaagtc agcgtctgat taccgcgact gaaaacggta
     6301 atcagttgat gcagcaaaac attaaagtca aaaactggct ggagcgggcg ctgcaatcgg
     6361 aacgcaatat taaagagcag attgccgtcc tgaagggcag cctgctgttg tctcgtatcc
     6421 tttaccagca acaacaaacg ctgccctcgg cggatgaact ggaaaacatg accaaccgca
     6481 tcgcggattt gcgtctcgaa cagtttgaag ttaaccagca gcgtgatgca ctcttccaga
     6541 gcgatgcgtt cgtcaacaaa ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc
     6601 acgatgcgtt attgcaagtg gttgatatgc gtcgcgaatt gctggatcag cttaacaaac
     6661 agttgggtaa ccagctgatg atggccatta acctgcaaat caaccagcag cagttaatga
     6721 gtgtgtcgaa aaacctgaaa tccatcctga ctcagcaaat cttttgggtg aatagtaacc
     6781 gtccaatgga ctgggactgg atcaaagcgt tcccgcaaag cctgagagat gaatttaagt
     6841 cgatgaaaat cacggtgaac tgggaaaaag cctggcctgc cgtttttatc gctttcctcg
     6901 ctggtttgcc gctgctgttg attgccgggc tgatccactg gcgtctgggc tggctgaaag
     6961 cgtatcaaca aaaactggct tccgctgtgg gttccctgcg taacgacagc cagctcaaca
     7021 caccaaaagc gatccttatc gacctgatcc gtgcgctgcc ggtgtgcctg attattctcg
     7081 cggttggcct gattctgttg accatgcagc tcaacatcag cgaactgcta tggtcgttca
     7141 gtaaaaaact ggcgattttc tggctggtgt ttggcctgtg ctggaaagtg ttggagaaaa
     7201 acggcgttgc cgtgcgtcac tttggtatgc cggaacagca gaccagccac tggcgtcggc
     7261 aaattgtccg catcagtctc gcgttgctgc ctatccattt ctggtctgtg gtggcagaac
     7321 tttccccgct gcatctgatg gatgatgtgc tggggcaggc gatgattttc ttcaacttgc
     7381 tgctgattgc cttcctggtg tggccgatgt gccgcgaaag ctggcgtgat aaagagtcgc
     7441 acaccatgcg actggtcacc attaccgtac tgtcgattat cccgattgcg ttgatggtgc
     7501 tgaccgccac gggttatttc tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg
     7561 tttatttggt gatcatctgg aacctgctgt accagacggt attgcgcggc ttaagcgtag
     7621 cggcgcggcg tatcgcctgg cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg
     7681 gcgcagaagg tgctgaaccg ccggaagaac ccaccattgc actggagcag gttaaccagc
     7741 agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa
     7801 tttggtccga tttgatcacc gtgttcagct atctggacag catcacgctc tggcattaca
     7861 acggcactga agctggtgct gcggtggtga aaaacgtcac catgggcagt ctgttgtttg
     7921 cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt ttgctggaag
     7981 tgttggtgct ctcgcgcctg aacatgcgcc agggcgcgtc gtatgccatt actaccatcc
     8041 ttaactacat cattattgcc gttggtgcga tgacggtgtt cggatcgctg ggcgtctcgt
     8101 gggataaact ccagtggctg gcggcagcat tatccgtagg tcttggtttt ggtttacaag
     8161 agatcttcgg taacttcgtt tccggtttga tcattctgtt cgaacgtccg gtgcgcatag
     8221 gcgacacggt aaccattggt agcttctcgg ggacggtaag taagatccgt attcgtgcga
     8281 caacgattac cgatttcgat cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg
     8341 agcgtctgat caactggtcg ttgactgaca cgactacgcg tctggtgatc cgtctcggcg
     8401 tagcttatgg ctccgatctg gaaaaagtgc gtaaagtgtt gctgaaggcg gcgactgagc
     8461 acccaagggt gatgcacgaa ccaatgccgg aagtcttctt tacggcattt ggtgccagca
     8521 cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg tgaccgtagc cgtactgtcg
     8581 atgagctgaa ccgtactatc gatcagttgt gccgtgaaaa cgacatcaac attgccttta
     8641 accagcttga agtgcatctg cacaacgaga agggcgatga ggtgacggaa gtcaaacgcg
     8701 actacaaagg cgacgatccg acgccagcgg tagggtaata accaaagggg gcgacattta
     8761 gttgccccga gattactaac aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct
     8821 tatccggcct acgaaaccgc aagaattcaa tatattgcag gagcggtgta ggcctgataa
     8881 gcgtagcgca tcaggcagtt ttgcgtttgc ccacaatctt aggggacatt tagcggcccc
     8941 atttatttct cacttttccg cctcatcatc gcgcgttaat ttcttttcat aatcgcgttt
     9001 tacaatatcc agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagtagcac
     9061 aatcaaatcg acggccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc
     9121 attgctagcg ggttaaacgc gctaacctgt tttcgatttt ttccagcgca tggcggcagc
     9181 gtgccaggcg cgcttcatag gcttccactt cacgatgcag cgtctgctgt tccacgagat
     9241 cggtcgctcg tgccagacgg gctctacgtt cggcaaccat ctcacgtagc cgccgctcaa
     9301 aatcctgatg ctgaatacgt ttacgctgcc agcgggcaat tttcggtggt gcgctatccc
     9361 actcgcgtag cgaccaggct gtggcttcac gcgctatagc ctccagttgc gccgccagat
     9421 gttctgccag ccaggcgact tgcggcagct gttgctgctc aaccgcatga cgaagcgcag
     9481 ccagattatc gcccgcctca tcgagacaag cctgcagtgt tgtcgcacga gtctgaaaaa
     9541 gatgcctgtc aaaacgggcg cttagcgtgg caaattgcgc taccggggca caacgctgac
     9601 gcagcgtagc gagctgtcct tccagttttt ccagcagcag ggcggttttc acgataagac
     9661 ctcaatgaaa atgataattg ttatgctaaa gtagcaactc tgtaagctga caacactcaa
     9721 tatgcaacga atcattttaa tcatcattgg ctggctggcg gtagtgctgg gtacgctggg
     9781 cgtggtatta ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc
     9841 ccgttcttcc ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct
     9901 acgtttctgg cagaaacatc atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct
     9961 tattttgctc acgtttgcca tttctctgtg gttcgtccag atgccatggg tgcgcatcat
    10021 gttgctggta attctcgcct gtttgctttt ttatatgtgg cgaattccgg tgattgatga
    10081 aaagcaagaa aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta
    10141 cattctggcg ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg
    10201 atacgtttaa aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg
    10261 actgcacagc agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa
    10321 cccggcattc ttttccgtga tgtcaccagc ttactggaag acccgaaagc ttacgctctc
    10381 agcatcgact tgctggttga gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc
    10441 gaagcgcgtg gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta
    10501 ccggtccgta aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgacctggaa
    10561 tacggcaccg atcagctgga aatccacgtt gatgccatca aaccgggcga caaagttctg
    10621 gtggtggacg atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt
    10681 cgtctgggtg gtgaagtggc tgacgctgcg ttcattatca acctgttcga tctcggcggc
    10741 gaacagcgtc tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat
    10801 taattatcgc cagtcttgtg ctgcccacgc tacggacagc acaagatgtg cattcagcct
    10861 cgccgttctg acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc
    10921 agagcctgcc aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg
    10981 acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta
    11041 ttcatcatgc ttatcttttt tccggtaccc gtggcgtcgg aaaaacctct atcgcccgac
    11101 tgctggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg
    11161 ataactgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct
    11221 cgcgcactaa agtcgaagat acccgcgacc tgctggataa cgttcagtac gctccggcgc
    11281 gtggtcgttt caaagtttat ctcatcgacg aagtgcatat gctgtcgcgt cacagcttta
    11341 acgcactgtt aaaaaccctt gaagagccgc cagagcacgt taagtttttg ctggcgacga
    11401 ccgatccgca gaaattgccg gtgacgattt tgtcacgctg tctgcaattt catctcaagg
    11461 cgctggatgt cgagcaaatt cgccatcagc ttgagcacat cctcaacgaa gaacatatcg
    11521 ctcacgagcc gcgggcgctg caattgttgg cacgcgccgc tgaaggcagc ctgcgagatg
    11581 ctttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggcgg
    11641 tcagtgcgat gctgggtacg cttgacgacg atcaggcgtt atcgctggtt gaagcgatgg
    11701 tcgaggccaa cggcgagcgc gtaatggcgc taattaatga agctgctgcc cgtggtatcg
    11761 agtgggaagc gttgctggtg gaaatgctcg gtctgttgca tcgtattgcg atggtacaac
    11821 tttcgcctgc tgctcttggc aacgacatgg ccgccattga gctgcggatg cgtgaactgg
    11881 cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca
    11941 aagaattacc gtatgcgcca gaccgccgta tgggtgttga gatgacgctg ctgcgcgcgc
    12001 tggcgttcca tccgcgtatg cctctgcctg agccagaagt gccacgccag tcctttgcgc
    12061 ccgttgcgcc aacggcagta atgacgccaa cacaggtgcc gccgcaaccg caatcagcgc
    12121 cgcagcaggc accgactgta ccgctcccgg aaaccaccag ccaggtgctg gcggcgcgcc
    12181 agcagttgca gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta
    12241 cccgcgcgcg gccggtgaat aacgctgcgc tggaaagact ggcttcggtc accgatcgcg
    12301 ttcaggcgcg cccggtgcca tcggcgctgg aaaaagcgcc agccaaaaaa gaagcgtatc
    12361 gctggaaggc gaccactcca gtgatgcagc aaaaagaagt ggtcgccacg ccgaaggcgc
    12421 tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagctg gcggcagaag
    12481 ccattgagcg cgacccgtgg gcggctcagg tgagtcaact ttcgctacca aaactggtcg
    12541 aacaggtggc gttaaatgcc tggaaagagg agagcgacaa ctcagtatgt ctgcatttgc
    12601 gctcctctca gcggcatttg aacaaccgcg gcgcacagca aaaactggct gaagcgttga
    12661 gcacgttaaa aggttcaacg gttgaactga ctatcgttga agatgataat cccgcggtgc
    12721 gtacgccgct ggaatggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt
    12781 ccattattgc ggataataat attcagaccc tgcgtcgatt cttcgatgcg gagctggatg
    12841 aagaaagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga
    12901 ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag
    12961 cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agtcaccggc
    13021 gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacacaactg ccgtcgcgta
    13081 gagatcgacc cgagcctgct ggaagacgac aaagagatgc tggaagacct ggtggctgca
    13141 gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcctctgta
    13201 tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc
    13261 tgttaacaca gcttatggaa gcactgcgct gtctgccggg cgttggcccg aagtcggcgc
    13321 agcgtatggc gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg
    13381 cgctcacccg ggcgatgtcg gaaatcggcc actgcgccga ttgccgcacc ttcaccgaac
    13441 aggaagtctg taacatctgt tcgaatccac gtcgtcagga aaacggtcaa atctgcgtgg
    13501 tggagagtcc ggcggacatt tacgccattg agcagacggg gcagttttca ggtcgttatt
    13561 ttgtgttgat gggacacctg tcaccgctgg acggcatcgg tcctgatgat atcgggcttg
    13621 atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaacc
    13681 ccacggttga aggtgaagct accgctaact acattgccga gctttgtgcg caatatgacg
    13741 tggaagccag ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg
    13801 gcaccacgct gtcacattcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga
    13861 gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct ccctcatcct
    13921 gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt
    13981 ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat
    14041 aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagctgcgt
    14101 ttccgcgcgc tttctaaccc ggacctgtac gaaggtgatg gtgaactacg cgttcgtgtc
    14161 tctttcgata aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacccgc
    14221 gacgaagtga ttgaccatct ggggactatc gctaaatccg gcaccaaatc attcctcgaa
    14281 tccctgggtt ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc
    14341 tactctgcgt ttatcgtggc tgacaaagtg accgtgcgta ctcgcgcagc aggcgaaaaa
    14401 ccagaaaatg gcgtcttctg ggaatcggct ggtgaaggtg aatacactgt tgccgacatc
    14461 accaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc
    14521 ctcgatgact ggcgtgtgcg ttccatcatc agcaaatatt ccgaccatat cgcgctgccg
    14581 gtagagatcg aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg ggagaaaatc
    14641 aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtacaaa
    14701 gagttctaca aacacatcgc ccacgacttt aacgatccgc tgacctggag ccacaaccgc
    14761 gttgaaggta agcaggagta caccagcctg ctgtacattc cgtctcaggc accgtgggat
    14821 atgtggaacc gcgatcataa acacggtctg aaactgtatg ttcagcgtgt gttcatcatg
    14881 gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc
    14941 agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac
    15001 ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac
    15061 gcggaaaaat accagacctt ttggcaacag tttggcctgg tactgaaaga aggtccggcg
    15121 gaagatttcg ctaaccagga agcgatcgcc aaactgctgc ggtttgcgtc tacccatacc
    15181 gattcttctg cgcagaccgt gtctctggaa gactacgttt cccgcatgaa agaagggcag
    15241 gagaaaatct actacatcac tgcagacagc tacgcggcag cgaagagcag cccgcacctg
    15301 gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg
    15361 atgatgaact atctgactga gttcgacggt aaaccgttcc agtctgtgtc taaagttgac
    15421 gagtcgctgg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca
    15481 ctgactccgt ttatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa agatgtccgt
    15541 ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc
    15601 actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc
    15661 ttcgaactga acccggatca cgtactggtg aaacgtgcgg cagatactga agatgaagcc
    15721 aagttcagcg agtgggtaga actgctgctg gatcaggcgc tgctggcaga acgcggcacg
    15781 ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtcat
    15841 gccggatgac ctttgtgtca tccggcattt ttcttttcat catctgcact ttccgcaaat
    15901 tatctcgcca ttaaccgttt cagccccagg tgcctttctt gaggcaatcg cctgttggtg
    15961 gtatcgttta tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt
    16021 atcattctgc ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag
    16081 aaatatggta ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc
    16141 tccgagctgg gtaaacaagc aaaagacatt atggatgctg gcaaactggt caccgacgaa
    16201 ctggtgatcg cgctggttaa agagcgcatt gctcaggaag actgccgcaa cggtttcctg
    16261 ttggacggct tcccgcgtac cattccgcag gcagacgcga tgaaagaagc gggcatcaat
    16321 gttgattacg ttctggaatt cgacgtaccg gacgaactga ttgttgaccg tatcgtaggc
    16381 cgccgcgttc acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta
    16441 gaaggcaaag acgacgttac cggtgaagag ctgactaccc gtaaagatga tcaggaagag
    16501 accgtacgta aacgtctggt tgaataccat cagatgactg caccgctgat cggctactac
    16561 tctaaagaag cggaagcggg taacaccaaa tacgcgaaag ttgacggcac caagccggtg
    16621 gctgaagttc gcgcagctct ggaaaaaatt ctcggctaat caagccgcag cagatctgct
    16681 ttcgggcagg tctgctcatc ctcctcaggc aatacctctc acagcaatta gttcttcttc
    16741 ttcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaga
    16801 caaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg
    16861 taaaacgcta tctgaaacaa tttttaagcg acagacgtgt ggttgatacc tcacggttgt
    16921 tatggtggcc attgctgcgc ggcgtgattt tgccactgcg ctcgccgcgt gtggcgaagc
    16981 tgtatgcctc tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac
    17041 agcaggcgct ggcacaacgt ttaccggaga cgcccgtagc gctgggaatg agctacggct
    17101 cgccatcact ggaaagcgcc gtagatgaac tactggcaga gcatgtagat catattgtgg
    17161 tgctgccgct ttatccgcaa ttctcctgtt caacggtcgg tgcggtatgg gatgaactgg
    17221 cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt gattacgccg
    17281 ataaccacga ttatattaat gcactggcga acagcgtacg tgcttctttt gccaaacatg
    17341 gcgaaccgga tctgctgttg ctctcttatc atggcattcc ccagcgttat gcagatgaag
    17401 gcgatgatta cccgcaacgt tgtcgcacaa cgactcgcga actggcttcc gcactgggga
    17461 tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tgggcgggaa ccctggctga
    17521 tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggtgtaggt catattcagg
    17581 tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa
    17641 accgtgaggt cttcctcggt gcgggcggga aaaaatacga atatattccg gcacttaatg
    17701 ccacgccgga acatattgaa atgatggcta atcttgttgc cgcgtatcgc taaagctgag
    17761 cggtaaagaa ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc atccgtgaat
    17821 aatgcaaaaa ggcgtgcagc gtgcctgggt agagtttgaa ctcacagggc tgctgatgcg
    17881 ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac tccgccccgg
    17941 caataaaaca gggcggaact tcgcgagtga gatcattatt aaacaggcag taatacggcg
    18001 actcgcggtc cgcgtcgttg cttaaatacg cctcttcata catctgtaaa tcctgttgcg
    18061 ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt aatccgtaaa
    18121 gcccatacca cagcaaaacg cccgcaactt taccgcaatc gatctgttta tcacgcaacc
    18181 acaacgcact ggcgagcgcc aacatggcac ctgcggaatc accggtaaag ccaatgcggg
    18241 acatattgat ttgataatct tccgcctgct ggtggaaata acaacaggca gccacaattt
    18301 cctctattgc ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaatg ccaatcaccg
    18361 tacattggct gtagcttgcc agcaggcgca tgatgcgatc gtgggtatca agattgccga
    18421 gaataaaacc gccgccatgc aaataaaata gcgtcgccgg gctatctggc tgcggacaaa
    18481 agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctctg gttgccattt
    18541 ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt tgctcagcaa
    18601 tcgttcccgt tgcgggccag ggcggtaaat ccggctgaag agtattcaca acggtcttca
    18661 tttcagcaga aataaggtcc agaacgggta atttgttttc cggcttcatg caaaaactcc
    18721 tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg cctgctgata
    18781 ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg ccgctcagtt
    18841 aaccacccgt aaaaacaacc atgaaatttc ccggcaaacg taaatccaaa cattattttc
    18901 ccgtaaacgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa accagcgctg
    18961 cctgggtagt gggtatcgat caaacgctgg tcgatattga agcgaaagtg gatgatgaat
    19021 ttattgagcg ttatggatta agcgccgggc attcactggt gattgaggat gacgtagccg
    19081 aagcgcttta tcaggaacta aaacagaaaa acctgattac ccatcagttt gcgggtggca
    19141 ccattggtaa caccatgcac aactactcgg tgctcgcgga cgaccgttcg gtgctgctgg
    19201 gcgtcatgtg cagcaatatt gaaattggca gctatgccta tcgttacctg tgtaacactt
    19261 ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt cgttgcttta
    19321 cgctgattgg cgagtccggg gaacgtacct ttgctatcag ccccggccac atgaaccagc
    19381 tgcgggctga aagtattccg gaagatgtga ttgccggagc ctcggctctg gttctcacct
    19441 cttatctggt gcgttgcaag ccgggtgaac ccatgccgga agcaaccatg aaagctattg
    19501 agtacgcgaa gaaatataac gtaccggtgg tgctgacgct gggcaccaag tttgtcattg
    19561 ccgagaatcc gcagtggtgg cagcaattcc tcaaagacca cgtctctatc cttgcgatga
    19621 acgaagatga agccgaagcg ttgaccggag aaagcgatcc gttgttggca tctgacaagg
    19681 cgctggactg ggttgatctg gtgctgtgta ccgcagggcc aatcggcttg tatatggcgg
    19741 gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg ggtgctatag
    19801 cggaatttaa ccagtatgag tttagccgcg ccatgcgcca caaggattgc cagaatccgc
    19861 tgcgtgtcta ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc atgaacacta
    19921 atggagcagg ggatggcgca ctggcagcgt tgctgcatga cattaccgcc aacagctacc
    19981 accgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact tattcatcgt
    20041 tagcgcaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac cagcattcac
    20101 ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct tactgggatc
    20161 gttaagttat cgaccagacc ggacgacgat ggcatccgta ggccggataa gatgcgtcag
    20221 catcgcatcc ggcaatggaa tcaatgtttt accccgtcac cacctcaccc gccggtggcg
    20281 tttccagcag ttccagcata gtacgagcaa tttcacgctc acccatcact acctgattcg
    20341 caccacgttc ggtgatatac gccacttcat cgtcataatg ggcgcgggca ataatctcaa
    20401 tatccggatt tttcgcgcgg gcagatgcca caatctcgcc cgcttcataa ccgttgggaa
    20461 tcgtcaggat cagccatttt gcacattcca gatgcgccag ttgcataatt tcttcgttcg
    20521 ccgcattgcc caatactgcg cggaccccgc gctctcgcag ttcatcaaca cgggttcgtg
    20581 acgtctcaat caccaccagc ggaatatcag aggcgagcaa tttctccccc agcaggctgc
    20641 ctacacgacc gtaacctacc agtagcgcat ggttgcaaat atccactggg atctgcttct
    20701 cttcttcgat agcctcttcc agcgtctgct cttccagcgt ttcggtcttc gccagatatt
    20761 tctccagcag cgcaaacagg accgggttga gcataatcga caaaatcgcc cctgccagta
    20821 ccaggttctg cccggcttgc ggcaatagat ccagcgccat gcccagtccc gccaggataa
    20881 acgcgaactc accaatttgc gccaggctgg cggcgatagt taatgccgta cgttgggagt
    20941 gaccaaacag tcgcaccagg aaaaacgcgg ctaacgactt accaaacaga ataatcgcca
    21001 gcgtcgccag cactgccagc ggttgctgaa tcagaattaa cggatcaaac aacatcccga
    21061 cggagacaaa aaacagcacc gcaaacgcgt cgcgcaatgg cagcgtatcg tgggcggcac
    21121 ggtgactcag ttcagactcg ttcagtacca tcccggcaaa gaacgcaccg agtgcaaagg
    21181 agacatcaaa cagctccacc gcaccaaagg caatccctaa cgccagcgcc agcaccgaca
    21241 gggtaaacag ctcacgggaa ccggttgctg cgctacgtgc cataatccac ggcaccagac
    21301 ggcgacctac cagcatcata atggcgataa atgcgatcac cttgccgatg gtgatcccca
    21361 tatcgacagc aagagtggca aagcccacat cgccttgttc catcattcct gccactgcgg
    21421 gcagcaacac cagcgtcaga accatgacca ggtcttccac aatcaaccaa ccgatggcga
    21481 tttgcccacg ctgactgtca attaattgcc gttcttcaag tgcgcgcagt aacaccacgg
    21541 tactggcggt ggaaaggcat aagccgaaca cgataccggt cattaacgac cagcccagca
    21601 cggcggagag cgccataccc agcagcgtcg ccacggctat ctgggcgatc gcaccgggaa
    21661 tggcgatggc ctttaccgcc atcaaatcct tcagcgaaaa gtgcagaccc acgccaaaca
    21721 tcaacagaat gacgcccagt tcagccagtt ccggcgcaag cttggtatcg gcaacaaagc
    21781 ccggagtgaa tggtcctgcc agcacacccg ctaacagata tcccaccaga ggagaaatac
    21841 gtagtttatt ggccagcatg ccgaggataa aggcgagcac aaggccgcca acaatggtgg
    21901 tgataagcgg ggtggcgtga tgcattccgt ctccttttcc tggtggttat tgtccatttt
    21961 tggccgggaa aaccaaaatt acaggtaata gtttatgaca atttcattga tgatgtttat
    22021 gaataattgt tgaattttgc agaaaaatgg aattagctgc aaaaaaagca cggatcggaa
    22081 aacagaaggc ggtttaacag aggaaaggct tatggcgact gtaaaacgat gcagccaaag
    22141 tttgcctttg gctgcaatga aatcagtctt tatgccggtt atcaggcagg aatatggtca
    22201 acatccccaa tagtggcagg aaagcacaga ttttatagac taactcgatg ctggtgtgat
    22261 cggcgataag ccccagaact gccgctccca gacctcccat gccaaaagca aaaccgaaaa
    22321 agagtccaga aaccataccg atacgtcccg gaagtagctc ctgagcgtag accagaatgg
    22381 cagagaatgc cgaagcgagg ataaatccaa taatcaccgt taaaaccccc gtccagtgca
    22441 ggctggcgta gggtaaaatc agcgtaaacg gcgcaacgcc gaggatagag ccccaaatca
    22501 catatttccg tccaatttta tcccctacag gcccgccaat caccgtacct gccgcaacgg
    22561 caaacaggaa ggcaaacaaa tgaagctgag cattctggat agataatccg aatttttgca
    22621 tcagataaaa ggtgtaatag ctgctgatgc tcgccatata gaaatatttc gagaaaatga
    22681 ggattaacag aatgctgacc gccagaacaa ctttattgcg cggcagtgga ttgataatcg
    22741 tcgctttggg ttttccttta ttcattcggt gctgtgccga gtaccaacgg ctgatttgcg
    22801 ccaacaccac gatcgccagc agtgccgcaa gcacaaacca ggcaacgttg cctttgccat
    22861 aaggcgcgat aatcaccgcc gccagcaagg ggcccaggga actgccaaag ttgccgccga
    22921 cctgaaagat agattgcgcc aggccatgac gcccgccgga agccatacgg gccacgcgag
    22981 aagattccgg atgaaagacc gatgaaccgg taccgaccag cgccgctgcc agcagaactg
    23041 cgccgaaact gcccgccagc gccagcagca ccagaccact taaggtaaag cacatgccaa
    23101 ttggcaacga ccacggcatc ggatatttat cggtccagta gccgacaact ggttgcagta
    23161 gcgaagaggc gagctggaag gtgagggtta tcatgccaat ctgcataaat gtcagagaaa
    23221 actctgactg aagcagcgga taaatcgcca gaatcagcga ttggatcatg tcgttcagca
    23281 gatgtgagag gctgatagca cctaaaatac caaacgatgt tcgggccttg gtcgttgacg
    23341 cagccgcgcc cgccacaggc tgggtttgtt cactcattgc cataggaaag tcactttttc
    23401 agggttgcga tgtaaagaat gatcttattt gtgattatta cccgactaac atacctgtat
    23461 gcgccgcctg aaggaagtct caacgctgaa tacagaattt ctaatctgga tacagattta
    23521 tcttcaccgg acgcagactt gtctatgatg tcgcgtcata ctatttttca acacgttgaa
    23581 atcaggtcag ggagagaagt atgaaattat tgcagcgggg cgtggcgtta gcactgttaa
    23641 ccacatttac actggcgagt gaagctgctc tggcgtatga gcaggataaa acctacaaaa
    23701 ttacagttct gcataccaat gatcatcatg ggcatttttg gcgcaatgaa tatggcgaat
    23761 atggtctggc ggcgcaaaaa acgctggtgg atggtatccg caaagaggtt gcggctgaag
    23821 gcggtagcgt gctgctactt tccggtggcg acattaacac tggcgtgccc gagtccgact
    23881 tacaggatgc cgaacctgat tttcgcggta tgaatctggt gggctatgac gcgatggcga
    23941 tcggtaatca tgaatttgat aacccgctca ccgtattacg ccagcaggaa aagtgggcta
    24001 agttcccgtt gctttccgcc aatatctacc agaaaagtac cggcgagcgc ctgtttaaac
    24061 catgggcgct gtttaagcgt caggatctga aaattgccgt tattggcctg acgacggatg
    24121 acacagccaa aattggtaat ccggaatatt tcactgatat cgaatttcgt aagcccgctg
    24181 atgaagcgaa gctggtgatt caggagctgc aacagacaga aaagccagac attattatcg
    24241 cggcgaccca tatggggcat tacgacaatg gtgagcacgg ctctaacgca ccgggcgatg
    24301 tggagatggc acgcgcgctg cctgccggat cgctggcgat gatcgtcggt ggccactcgc
    24361 aagctccggt ctgcatggcg gcagaaaaca aaaaacaggt cgattacgtg ccgggtacgc
    24421 catgcaaacc ggatcaacaa aacggcatct ggattgtgca ggcgcatgag tggggcaaat
    24481 acgtgggacg ggctgatttt gagtttcgta atggcgaaat gaaaatggtt aactaccagc
    24541 tgattccggt gaacctgaag aagaaagtga cctgggaaga cgggaaaagc gagcgcgtgc
    24601 tgtacacccc tgaaatcgct gaaaaccagc aaatgatctc gctgttatca ccgttccaga
    24661 acaaaggcaa agcgcagctg gaagtgaaaa taggcgaaac caatggtcgt ctggaaggcg
    24721 atcgtgacaa agtacgtttt gtacagacca atatggggcg gttgattatg gcagcacaaa
    24781 tggatcgcac tggtgccgac tttgcggtga tgagcggagg cggaattcgt gattctatcg
    24841 aagcaggtga tatcagctat aaaaacgtac tgaaagtgca gccattcggc aatgtggtgg
    24901 tgtatgccga catgaccggt aaagaggtga tcgattattt gaccgccgtc gcgcagatga
    24961 agccagattc aggtgcctac ccgcaatttg ccaacgttag ctttgtggcg aaagacggta
    25021 aactgaacaa cctgaaaatc aaaggcgaac cggtcgatcc ggcgaaaact taccgcatgg
    25081 cgacattaaa cttcaatgcc accggcggtg atggctatcc gcgccttgat aacaaagcgg
    25141 gctatgtgaa taccggcttt attgatgccg aagtgctgaa agcgtatatc cagaaaagct
    25201 cgccgctgga tgtgagtgtt tatgaaccga aaggtgaggt gagctggcag taatccgaaa
    25261 gtgccggatg ttcgcatccg gcacaatgct taatcgcgac gggcgatatc agcaaatttg
    25321 gcatcgagga tctttgccag atcgcctgcc gccagttcga tatccagccc gcgcttgccg
    25381 ccggaaacat aaatagtggc aaattcttgt gcgggggcgt cgataatcgt tggcagacgt
    25441 tttttctgcc ccagtgggct aatcccccct accagatacc ccgtcgaacg ctgcgcgacc
    25501 atcggatcgg ccatctcaac tttcttggca cccagcgctt ttgctacttt tttaagatcc
    25561 agttgacctg cgaccggcgt aacggccacg gcaaggtgtt tcatatcacc gttcactgcg
    25621 accagcagcg ttttgtagac ctgatccgga ttcaaaccta attttttgac gacttcatcg
    25681 ccaaaattgg tttcagccgg atcgtgctcg taggtgtgga tttgaaacga aatcttgttt
    25741 ttttcgagta atttaactgc gggtgtcatg gtaattttcc tgccttaaac aaaaaaacga
    25801 agcaagcata cgcctgatgg cggtccaaaa aaaagagtca tcttgcctaa gagtattggc
    25861 aggatggtga gattgagcga caatcgagtt acaccgtcgc ttaaagtgac ggcataataa
    25921 taaaaaaatg aaattcctct ttgacgggcc aatagcgata ttggccattt ttttagcgca
    25981 acatctgcgg caaattccct tctccataca ggtgtagtgc accaaccgcg accacatagc
    26041 gccccggcgg catggcgcat aatttgtccc gccaggcgag atttcgctga tgcatcagca
    26101 catcgtacag cgactgactg aacgtattag gcagcgttat ttcattattt tgcggcggcg
    26161 cattcagcca ccagctcatc atttgttgca gcaaccgtgc gttggtgtgc cagtgggtca
    26221 gcgtatcgtc cagcagcgcc agtccgttgt cagggagctg gagcaacatg gcaatctggt
    26281 tttcagcccc ttccagttca atcacgggtt tatgttgttg cttagccgcc tgcaatagct
    26341 ggtaatcgat accgtattct gcccgcagcc ccagtttttg cgcctgcgtc gcctgaagaa
    26401 ccatcgcgat ttgccacagc ggttgagtag aaaagagcga cggagaaatg cccatctcct
    26461 ggctaatatg ctgcaggttt tggagttgct cctcgctaat gcgcttttcc agggcctcgc
    26521 aggcaggcaa attggcaaaa ggcgtatcgc tggtggaaac atccgcctcg acgatcagcg
    26581 catcggcgtt tttgagcttt ttgagcaaac gggtgggcag gggagccata tcgtggctac
    26641 ccatatgaat actgccgatc agatgaaaat ggcgattgcc gggaagagag atatcgatag
    26701 caggccaggt gtaatgatta ccgcgcagcg cggcccaaag cgtttttacc cggtacaaca
    26761 gatccatacg acctcccttt gtgaaatatc atgctagcgc gcggggaggg atggcgcaaa
    26821 tggagttgat tgttgcactt gcgcacagtg cttgtgtgca ttacaatgat taaataatgg
    26881 acaaggagat gagagaggaa tggctcaaaa gaaaaatata aggagttttc gtgatgcctg
    26941 gttggctgat ttttttgtcc attcaacgcc acatcgaaag atccctgctg aaatccatac
    27001 cacgttatca cgaaagttgg acatcattaa tgctgccact tctcatcggg atttacgctc
    27061 ccctccgggg aaccgatacg aagagttatc cgggaaatta caggaatact cctccattag
    27121 agtgaataaa cagtaccgac tgatttttaa atgggttaat ggcaaggccg aagaattgtt
    27181 tttagaccca cacaactact aaaacatagc tgtcgtattg acgagaaagg taaaactatg
    27241 aaacaggcaa caagaaaacc gacgacaccc ggagatattc ttctctatga atatctggaa
    27301 ccgctcgatt tgaaaatcaa tgagttagca gagttgctgc atgttcatcg taatagcgtc
    27361 agtgcactga tcaataacaa tcgtaaactc accactgaga tggcattccg tctggcgaaa
    27421 gtttttgata ccacagtcga tttttggcta aacctccagg cggcagttga tctttgggaa
    27481 gttgaaaaca acatgcgtac ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat
    27541 ttggcacgcc gtgaagagcg tgcaaaaaag gtcgcgtaga ataatttccc tataacggat
    27601 gcgccaccct aaagcagtgc atccgcaatg atgtacttat tccttcggtt taaaccgcag
    27661 caaccggttg gcgttactca ctacggtaat cgacgagagc gccattgccg ctccggcgac
    27721 caccgggtta agcagcgtac cggtgaacgg ccacaaaata ccggcggcga ccggaatacc
    27781 aatgctgttg tagataaacg caccgagcag gttctgcttc atattgcgca gcgttgcgcg
    27841 ggaaatcgcg agcgcatccg caacacccat caggctatgg cgcatcaggg taatcgccgc
    27901 ggtttcaatg gcaacatcac tgccgccacc cattgcaatg ccgacatccg cctgagccag
    27961 cgctggcgcg tcgttaatgc catcgcccac cattgccacc tgacgtcctt cactttgcag
    28021 acgtttgatc gcttcggctt taccgtccgg cagcaccccg gctatcacct catcaatccc
    28081 tgcttctttg gcgatcgcat tggcggtggt tgggttatcc ccggttagca tcaccagacg
    28141 atatcccgct ttatgcaggc gttgcagcgc cgccacgcta tcactacgca atggatcgcg
    28201 tacagccagc agggctaccg ctttcccgtc aaccgccagc agcacaggcg ttgccccttg
    28261 cgatgcctga gctgaaatat ctgcttcaat cgctttagta tcaacttgct ggtcatttaa
    28321 cagcgcctga ttgcccagca ataacgcatg accttcagct tcaccgctca cgcccagccc
    28381 gcgcaatgtg cggaaaccgt tgacctgcgg cagctgcata tcgcttgctt tatcgaggat
    28441 cgcccgtgcc agcggatggc tggaaccttg ttccagtgcc gccgccagac gcagcgcctg
    28501 cgcttcgtca aaatcagcaa atgtttttac tgcgacaacc tgcggcttcc cttcggtcag
    28561 cgtcccggtt ttatcgaaca ccacggtgtc gagcgtactg gcgcgttgca gcgcgtcggc
    28621 gtcccgcacc agcacgccaa actcagccgc ccgcccgacg cccgaaataa tcgacatcgg
    28681 cgtcgccagt cccagcgcac acggacaggc gataatcagc accgtggtgg caatcaccag
    28741 ggtataaaca atttgcggtg ccgggccaaa gaaataccag attgccgcac tgaccagcgc
    28801 aatgactacc accaccggca caaacacagc ggagatttta tccgccagtt gaccgatttc
    28861 tggcttgctg ctctgggcct gtcgcaccat gcgaatgatc cgtgacagcg tagtatggct
    28921 gccgaccgca ctggcgcgaa acagcacact gccgtcctgt accactgtcc cggcatggac
    28981 gctatcgcct tcgccttttt gctgcgggat tggttcgccc gtcagcatcg cttcatccag
    29041 ccacgcttcg ccctgggtaa tttcaccatc taccggcacc cgatcacccg tcgtcaggcg
    29101 cagcaacata cctggctgaa catctgccag cggcacgttt ttttcacctt cgttggtgac
    29161 caggcgcgcc gtcggtgggg ttaaatcgag taatttctcc agcgccttag aagaacgctg
    29221 acgtgcgcgc gcttccagca tatgaccgag attaatcaga ccgataatca tcgcactggc
    29281 ttcatagtaa agatgtcgcg cttccatcgg gaaccactgc ggccacaggt tgacgctcat
    29341 tgaatagagc cacgccacgc ccgtacccag cgccaccagc gtatccatcg tcgctgcacc
    29401 gttcagcagg cttttccatg cgctgcggta aaaatggccg ccggcgaaaa ccattaccgc
    29461 cagggttatc aggccgataa ccaaccacag gctgcggttg tcggcggtga ccatcatgtt
    29521 atcgccgatc atcccccaga ccatcaccgg gatacccacc gccagtgcga caatcgcctg
    29581 ccagcggaag cgcttcatcg tagcgacggc ggtttcttgc tggcgctcgc ggcgtttagc
    29641 gtcatcttca atcgcttccg cgccgtagcc cgctttttcc accgcctgca ctaaatcttg
    29701 tggggaggca ctgcccatca ccagcgcagt acgctccgcc aggtttaccc gtgcctgagt
    29761 gacgcccggt acgctttgca acgcattttg tacgcgggtg acacagctgg cgcagctcat
    29821 gccgctcagc agcaactgct ggctgtcatc gtcatcggcg gtcgctgccg gaagcgcctc
    29881 agaaaccgct gtcagtgctt ccgacgggat tgatgactcc gccagcggtt tagcctttgg
    29941 gtggcttaca gatgcgtcat aacccgcttg tttgatggtt tcgatcagct gttctgcact
    30001 ggcagtcccg gtaacgtgcg cttcagtgac agacacatcc gcctgctcaa catccgggcg
    30061 ctgttcgaga ctttctttca cgcgtttaac gcagtgaccg caggacaggc cgtccagggt
    30121 caggtcgata gtttgtgaca taaaacactc cttttaagac agttttgact ggctgtgata
    30181 aaggttaaac cttccagcaa ggggaaggtc aagaaattaa taaaccaggc gggtaaaagt
    30241 acgtaaagat taaaaaatcg gctcgatttg catcaggatt agacatttat ctctttgttt
    30301 tcctgtagtt aagttgcggg tgctaagtta aatcaggatg cctgaaaatt ggcaccgggg
    30361 tgaggaatta cctcccgcat ctataaaaag gagttaacaa aagatgttag atgcaaacaa
    30421 attacagcag gcagtggatc aggcttacac ccaatttcac tcacttaacg gcggacaaaa
    30481 tgccgactac attccctttc tggcgaatgt acctggtcaa ctggcagcag tggctattgt
    30541 gacctgcgat ggcaacatct atcgcgcggg tgatagtgat taccgctttg cgctggaatc
    30601 catctcgaaa gtctgtacgt tagcccttgc gttagaagat gtcggcccgc aggcggtaca
    30661 ggacaaaatt ggcgctgacc cgaccggatt gccctttaac tcagttatcg ccttagagtt
    30721 gcatggcggc aaaccgctgt cgccactggt aaatgctggc gctattgcca ccaccagcct
    30781 gattaacgct gaaaatactg aacaacgctg gcagcgaatt ttacatatcc aacaacaact
    30841 ggctggtgag caggtagcgc tctctgacga agtcaaccag tcggaacaaa caaccaactt
    30901 ccataaccgg gccattgcct ggctgctgta ctccgccgga tatctctatt gtgatgcaat
    30961 ggaagcctgt gacgtgtaca cccgtcagtg ctccacgctc atcaatactg ttgaactggc
    31021 aacgcttggc gcgacgctgg cggcgggtgg tgtgaatccg ctgacgcata aacgcgttct
    31081 tcaggccgac aacgtgccgt acattctggc tgaaatgatg atggaagggc tgtatggtcg
    31141 ctccggtgac tgggcgtatc gcgttggttt accgggcaaa agcggtgtag gtggcggtat
    31201 tctggcggtc gtccctggcg tgatgggaat tgccgcgttc tcaccaccgc tggacgaaga
    31261 aggcaacagt gttcgcggtc aaaaaatggt ggcatcggtc gctaagcaac tcggctataa
    31321 cgtgtttaag ggctgatcat gatgaacacg gaaggtaata acgataacaa acccctcggt
    31381 ctatggaacg tcgtttccat cggtattggg gcaatggtgg gggcggggat cttcgcgctg
    31441 ctggggcagg ctgcgttgct aatggaagcc tcgacctggg tcgcctttgc ttttggcggt
    31501 attgtggcga tgttttccgg ttatgcctat gcgcgcctgg gggcgagcta tcccagcaat
    31561 ggcggtatta tcgacttctt tcgtcgcgga ttaggcaacg gcgtcttttc gctggcactc
    31621 tcgttattgt acctgttgac gctggcggtg agcatcgcca tggtcgcccg tgcttttggc
    31681 gcttatgccg tgcagttttt gcatgaaggc agtcaggagg agcaccttat attgctctac
    31741 gcgttgggga tcattgcggt gatgacgctt ttcaactcct taagcaacca tgcggtaggg
    31801 cggctggaag tgatcctcgt cggcattaaa atgatgatcc tgttattgct gatcattgcc
    31861 ggtgtctggt cgctgcaacc ggcgcatatt tccgtctctg cgccccccag ctccggtgcg
    31921 ttcttctcct gtatcgggat aaccttcctt gcctatgcgg gctttggcat gatggcgaac
    31981 gcggcggata aagtgaaaga tccgcaggtc attatgccac gggcgtttct ggtggcgatt
    32041 ggcgttacca cgttgcttta tatctcgctg gcgctggttt tgcttagcga tgtatcggca
    32101 ttagagttag aaaaatatgc cgataccgcc gtagcgcagg ctgcttctcc gctgctcggg
    32161 catgtgggtt atgtgatcgt cgtcatcggc gctttactgg cgacggcttc agccataaac
    32221 gcgaacctgt tcgccgtgtt taacatcatg gacaacatgg gcagcgaacg cgaactgccg
    32281 aagctaatga ataaatccct gtggcggcag agtacctggg gcaacatcat tgtcgtggtg
    32341 ctgattatgc tgatgacggc ggcgctgaat ttaggctcac tcgccagcgt tgccagcgcc
    32401 acctttttga tctgctatct ggcggtgttt gtggtggcga tccgcctgcg tcatgatatt
    32461 cacgcctcgt tgccgattct tatcgttggt acgttggtta tgttgttggt gatcgttggc
    32521 tttatctaca gtctctggtc ccagggcagc cgtgcgctga tatggattat tggcgcactc
    32581 ttactcagcc ttattgtggc aatggtcatg aagcgcaata aaactgtata acatctctct
    32641 gtgcgcagta cctcctgtat tattgtggtg gcggtcgata ttcgcactgg caaaaaaacg
    32701 tgcttgaata tctgttgaaa ccctttaaca aagcacagga ggcgttgcgc gaacgatgaa
    32761 cattagcgat gtagcaaaaa ttaccggcct gaccagcaaa gccattcgct tctatgaaga
    32821 gaaggggctg gtgacgccgc cgatgcgcag cgaaaacggt tatcgcacct acacgcagca
    32881 gcatctcaac gaactgacct tactgcgcca ggcacgccag gtgggcttta acctggagga
    32941 gagcggcgaa ctggtgaatc tgtttaacga tccgcagcgt cacagcgccg acgtcaaacg
    33001 tcgtacgctg gagaaagtgg cggagatcga acagcatatc gaagaactgc aatccatgcg
    33061 taaccagctg ctggcgctgg cgaatgcctg ccccggtgat gacagcgccg actgcccgat
    33121 tatcgaaaat ctctccggct gctgtcatca tcgggcgggg tgattaaggc ttgcgaaaat
    33181 agtcgtccat tattttcgtc agctccggac gttccccgtc atcattgatg gcaaaatcca
    33241 gaaaaaaagc atctgcttgc ggcgttcggt tttgcgccat caaccggaag gtgagggggc
    33301 ctgaccaggt ttcggatggg ctgatgtgct tatcagggcc gaaactctcc catgcgttgg
    33361 gcggtaatgt tttgcacgcg gtgagttttg gtatgtggta ttgcagctct gttttgtgca
    33421 gacattcccc gccaggaaat accagggttt gttgctcatc attaaaatgg gttgccgtgc
    33481 tgagcaccag tgttttgagc ggtcgccctt ccagcgcggc ggcagtagcc ttatggtgtc
    33541 cgtcgagcag aacgcagaga taggaatcag ggagatacca ggccagagcg cgggcgattc
    33601 cagttttcga gagcagacaa aaaccgggcg gctccctgtt caccctccgc aaaaccgctg
    33661 tagacaaatt ccgcacaggt agggcaacca gcctcggacc agaaacgtat taacgggaca
    33721 tccttgccag cagagcgaat ggataagacc gtgcgagtgc ggatcgtaaa tcacacattg
    33781 acgcagaaat cctttcccgg acttcaccga aaaaatgacg cccgcagcgg tatttttcag
    33841 cagacgtact tcaggttttc gaaaggggaa aaagcatggc cactccttag ccaaaatttc
    33901 agttccctga actgtgccag aaaattacgc tggtgactat tgctctggaa atggaaacag
    33961 tgtccttacc cattaatccc cgtttgtacc gaaagaatat gttgcaaact ttctaccata
    34021 atgcgccccg actgaaataa gccttcctgg atttcattgt taacattcac agcgtgaacc
    34081 agggaggcta ataacaccac ttgcaagaag gcaaaaataa acgcaccgat aatcataata
    34141 tgcgtcaggg ggattttcct ttgctccttc attttaaata acccacaaac tggccttctt
    34201 tcattaaggc gacttttcct gtgtgatgat aaacaatgac attatacaga cccgactgat
    34261 aaaccgtggc atcggcatta tcttcatcta tggcacataa tgaatcattg agataagcaa
    34321 aattcttact actcattcta aaggtcatgc caaatccttt ataaatggct ttttttgccg
    34381 cctgggcatg ttgtgcggct tctgcatttt taaatgcctg cgcttcagaa gcacttaaaa
    34441 tagccgtctg gcgctgctgt tctgcgagat aacaggtgtc atggctgacg ccctgcgcct
    34501 gacaatctgc gatgcgggaa gcgggtgagc tacagccagc cagtaatata accgccagca
    34561 gggaaaagga tttaatggag ctattcatca ttgttaacct taaataatgt gcgaattaat
    34621 gaagaggtga aacccggcaa gcttccttgc tcttatttat attgaggatt gaaatattag
    34681 aacgaatatc ctacgccgac acctgcgccg aaatcagatt gcgtatccgc agaaataccg
    34741 gctttaagta ccgtatgcgt tccggcatta aaagatgttc cgacagacac cgcagattca
    34801 ccgttgaagg ttcccgctcc cgctgacacc atcatcacgt caccggtttt aacctgtggt
    34861 tgtgaggcga tagcgatggc agatgcagtt cccgcattgg cttctttacg gttgtcctca
    34921 acttcgtttt tcagcgaact gtaatggtta ttgagcgtgt taatttgttg gctgttatct
    34981 gcgacccgtt tttgcgtgtt gcttaactgt tgctgcgttt gtgcggaatc ttgtctcaga
    35041 ctttcaattt gctcatgtgc ctggacagac ttctgcgcca gactttgcat ttgtttatcg
    35101 tattgagccc tttctttttg ctgttgtgct gccacatcgg ttgtcagtgc attcattttc
    35161 tgcccttgtt ccgtggtagt gcgctgatta gcctcatttt gagccgccag tctggcatta
    35221 atcatctcct gataatgcgc agtattttgc gtatctccag tcgcggcgag cgttttttgt
    35281 gtggctacga tctgcgcctg ttgctctgta atttgcgttt gctgggcatc agttaccgca
    35341 gactgtcggg cgttgatcat gctctgataa cgggcaggtg tctgtgtatc acctgtagcg
    35401 gcaacggttt tttgtcctgc gtcaggatct gtctggacag tgggagtctg agtggtatca
    35461 ggtgtttcag aagggatgta gtgatgttca gtcaaatagg tatacgcagc ggggctgttg
    35521 tctgtgcgtt ctaaaaagcg ggtaatcttc tgctcaatct ccgattgcga catgccagag
    35581 ttaatggctg aaagctctgt tgccgccgct tcaatggtat caccagctaa aacaacaggg
    35641 ctggatgttg ctgaaattat gagtgccagt aaagctacgt ttacagtttt cattcatttt
    35701 ctcttgttca aaataagtcg cattaatgtt tctataagaa acaaataata ttattttaag
    35761 aaataaactg cgttgcaatt taatcgaaat ctacgccgcc atttgtgtga tgtaaagcgc
    35821 atttttgtct ggtgtaagcg taaacaccac caataagaaa caaaaaatgt ttcttatata
    35881 aataaaatcg aaaatcattt tgtgctcttt caatgtaaaa aagagtaaag acttgttttg
    35941 ataagcgaaa taaattctgt ttctggacta aacgtgaagc ggttcctgat gtgtgctaaa
    36001 atgcgtttct ttgataaatc tcaattttta agaggtttac gccaggcagg aaaatttaaa
    36061 tgaatgaatt tgagaagatt tttaatgaaa tgaatctgga ccgggcattg ttaccgatcc
    36121 tttttcgttc gaaccggtcg acagtgtgga agtatttaag cggcgatagt acggctcctg
    36181 cctctgccat gtcgctgatc atgctgttgc agttgatcca gaaacgaaac cccgatctgt
    36241 tagccgaatg gttaacgctt tctgatttca ccattccgcc agaagtttac ctggaccaac
    36301 ctgattactg gaaagggtgg gtgtataccc aacacaaggt caataaaaac gtcctggaat
    36361 atctaaaaaa acactatccg gatgaagacc aaaaaagtat gggtaaaggc cgggaagagt
    36421 aattttgcct ctactcggct atatgccgga tgcggcgcaa gcgccttatc tggcctacga
    36481 ttcgggcata ttttgtaggc atgataagac gcgccagcgt cgcatcaggc gatgaccgca
    36541 cggataatca gcgttatccc ttctatcgca atcacctcaa catgcgtacc tgcactgaga
    36601 tcctcgctgg cgctgacagg ccatgaactg tcaccgacgc gcatatgacc acgcccgttg
    36661 accagcggag attccagaac aaaacgtcgg ccaatcaact gctgcccgcg ctggtttaaa
    36721 tgactgtcgc tgtgcttttg ttcgcgcacc cgccgcgaca accatttcca ccacagccag
    36781 gcggcgagca gcgtcaggac ggcaaacatt accccttgcc actcccaacc cagcggcagc
    36841 aaccagacca ccagaccgat aatcaccgct gccacgccgc tccacaacag ataaccattg
    36901 ccgcccaaca tctcggctgc cagcagcaaa ccgccgagac ttagccagaa aatatgtgga
    36961 tgaacgacca ttaactccat catggctgag tccgtttgtt ggcgctgtct ttcaccagct
    37021 cagcaatccc ggcaatcgac cccatcaggc tgctggcctc taatggcatc atcaccactt
    37081 tgctgttact ggacgaaccg atctgctgta acgcttcggt gtatttctgt gcgacgaagt
    37141 agttcaccgc ctgaatatca ccggaggcaa tggcttcaga caccatttta gtggcgcggg
    37201 cttctgcttc agcggaacgt tcacgcgctt cagcctgtaa aaacgccgac tgacgttcgc
    37261 cttccgcttt caggatttgc gactgttttt caccttcggc tttgaggatt tccgcctgac
    37321 ggatcccttc cgcttcaagg atgtaagcgc gtttggtacg ttccgctttc atctgcgcgt
    37381 tcattgaaga gataagctct gccggtgggc gcacgtcgcg aatttcaata cgggtgactt
    37441 taatccccca cgggttggtg gcctcatcga caatacgcag caggcgtgag ttgatgctgt
    37501 cgcgctgaga gagcatttcg tcaagttcca ttgaacccag cacggtacgg atgttagtca
    37561 tggtcaggtt gatgatcgcc agctccagat tgctgacttc ataagccgcg cgcggcgcgt
    37621 caatcacctg aataaagcac acggcgtcga tggtaacgtt ggcgttatct ttcgagataa
    37681 cttcctggga agggatatcg agcacttgct ccatcatatt gatcttgcga ccaatgcgat
    37741 ccataaacgg caccaccaga ctgagccccg gctgtaacgt tttggtatag cgaccaaaac
    37801 gttctactgt ccactgatag ccctgcggca cgattttgac gcccgcgccg acaatgacca
    37861 gcgcgacaaa aatgagaatc gggataaaga taagcatcgg aaaaacctcc tgttgtaccg
    37921 tccataatca gcaaaattgc tgcttgatta aacaaattat acctgattac tgaaagagag
    37981 ttccccctta ttcctgcgaa ggataaactg tttttagtaa aaatcagaaa aagggaacag
    38041 tgatgcagga aaatagtcct ttgcttcagc tacaaaacgt aggatatctg gcgggtgatg
    38101 cgaagattct taataacatc aatttttcgc tgcgtgctgg cgaatttaag ttaattaccg
    38161 gtccttctgg ttgtggcaaa agtacgctgc taaaaatagt tgcttcattg atcagcccaa
    38221 ccagcggaac gttactgttt gaaggtgagg atgtcagcac actaaagcca gaaatctacc
    38281 gccagtaagt ctcttattgc gcccagacgc cgacgctgtt tggcgacacg gtatacgata
    38341 atctgatctt tccctggcag atccgtaacc agcagcctga cccagccatt tttctcgatt
    38401 ttctcgaacg cttcgcctta ccggacacca ttttgacgaa gaatatcgcc gagctatctg
    38461 gtggtgaaaa acaacgcatc tcattgattc gtaacctgca atttatgccg aaggttttat
    38521 tgctggatga aataaccagt gcgctggacg aaagtaataa acataacgtt aatgagatga
    38581 tccaccgtta tgtgcgcgag caaaatattg ccgtgctgtg ggtgacacac gataaagacg
    38641 aaattaatca tgcggataaa gtgattacac tgcaaccgca tgccggagaa atgcaggaag
    38701 cacgctatga actcgcataa tattactaac gaatcattag cactggcatt gatgctggtg
    38761 gtggtggcaa tcttaattag ccataaagaa aaactggcgc tggagaaaga tattctctgg
    38821 agcgtcgggc gcgcgataat ccagctgatt attgtcggct atgtgctgaa gtatattttc
    38881 agcgtggatg atgccagcct gacattattg atggtgttat ttatctgctt taatgcggcg
    38941 tggaacgcgc aaaaacgcag taaatatatt gctaaagctt ttatctcatc gtttattgcc
    39001 attacggtcg gggcgggaat taccctggcg gtgctgattc tctcagggtc gattgaattt
    39061 atcccgatgc aggtgatccc tatcgccggg atgatagccg gcaacgccat ggtagcggtg
    39121 gggttgtgtt acaacaattt agggcaacgg gtcattagcg aacagcaaca gattcaggag
    39181 aaactgagtc ttggcgcgac gccgaaacag gcttcagcga tattgattcg cgacagtatt
    39241 cgcgcggctt taattccgac ggtcgattca gcaaaaacgg taggcttagt gagtttacca
    39301 ggaatgatgt ccgggctgat atttgccggg attgatccgg tgaaggcgat taaatatcag
    39361 attatggtga cctttatgct gctctcaacc gccagcttgt cgaccattat tgcctgctat
    39421 ttaacctatc gtaagtttta caattcgcgc caccaattgg tggtgacgca attgaagaag
    39481 aaatgatgcg atgccggacg cgcagcatcg catccggcgt tgtggttcat gtgccgatca
    39541 acggaaactc aatacaacaa cgcgtacagc tggcggcgat acttcgacgc cagtgcatca
    39601 cccgtaccca gcgcagcgag gatctcctgg aatgttttac gtgtctgacc atctgcggcg
    39661 gtgagatctt tacgcagatg cccaaacagt aactccagcg cctcttcatt gcgcccaacc
    39721 tggtgcagtt gcagtgccag ttgtgtcgcc agtgcggcat cttctggatt ctccgccacc
    39781 tgctgttgca actgttgaat ttccggcgta tcagccgcct gcttcagcag ttcgatttgc
    39841 gccaccagcc cctgatagcg ggtgtcctga tcctgcaacg gaatggtttt cagcacggct
    39901 tctgcatctt cagaacggtt cagcgcaatc agcgtttctg ccagcagcag gccgatctcc
    39961 ccgttctgat tcgacaactg ccaggcgtct ttcagcaatg gcaaggcgtc ggtgtaattg
    40021 ccttcctgca tcagctgcat cgcctgctgc gctttcagct cttcttcgcg cggcagcact
    40081 ttatccagca gggcgcggat cgcctcttcc ggttgcggcc cctggaagcc atctaccggt
    40141 tgcccgttct ggaacagata cacggtcgga atcgcgcgca gaccaaactg ggcggcaatc
    40201 atctgctccg cgtcgcagtc cagcttcgcc agaataaatt gcccgttgta ctgcgccgcg
    40261 aggctttcca ggatcggggt taactgcaaa cagtgctggc tacgttcaga ccaaaaatag
    40321 aacagcaccg gagtggtcat cgactgttca agaacctgtt gcaggttaga ttcgttaatg
    40381 ttgacaatat tttctacgga catggagtcg ctctctgttg tcgatatttt ctttgacatg
    40441 ggggcttaag cgcgcgcttc aactcacccc tgcaatattt tgtccatcac gcgccccggc
    40501 agcaggcgct taagcaccat taccgcccag gtcaccagcg ttaccggata gcgcatcttc
    40561 ggcttctcgc taataaaagc atggcgtact ttgtccacca ccgcttccgg tcccaacgta
    40621 aagcgggcgg cgatgccggg attttcgact ggtttatcac tttgcgtctg gttgacgttg
    40681 tcggtgaagc gagtacgaat gggaccgggt tcgatcaggc tgactttaat tccacagtgg
    40741 cgtagctcca tgcgcagcgc gtctgaccac gcctccagcg catatttact ggccgcgtaa
    40801 gcaccacgac ccggcgtgga gattaatccc atcaccgatg aggtcatcac aatacgccct
    40861 tcaccgtgcg gtaacatcgc gggtaacagg cgcatggtga gctggtgtgc gccgaaaaag
    40921 ttggcggaaa actgctgttc catctgcgca cggctgatgg tggaaagggg gccatacatg
    40981 ccgaatccgg cattgttaaa gatcccatac agacaattat cggtcagggc gatcacctcg
    41041 tcggctgcgc gatcaacact ttctggtgaa tccagatcga tcaacacgcc ggtaaatccc
    41101 atgctgttca tgcgctcaac atcatccggt ttccggcaac ctgccagcac atgaaaaccc
    41161 tggcgtttta attcgagcgc gctttccagg ccaattccac tggaacatcc ggtaattaag
    41221 accgattttt gcataacttt acctgtcagg atctccgttg ctttatgagt catgatttac
    41281 taaaggctgc aactgcttcg ccatccagtc ggcaataaac ggctgggcgt cgcggttggg
    41341 atgaataccg tcatcctgca tccattgtgg tttgaggtag acctcttcca taaaaaaggg
    41401 cagcagcgga acatcaaact ctttggcgag tttggggtaa atggcgctaa aggcttcatt
    41461 ataacggcga ccatagtttg caggcagacg tatttgcatt aacaatggtt cagcgttggc
    41521 ggctttgaca tcctgcaata tctggcgcag cgtttgctcg gtttgttgtg gctgaaaacc
    41581 acgcaaaccg tcattgccgc ccagttctac cagcacccag cgcggctgat gctgtttcag
    41641 cagagccgga aggcgcgcca gcccttgttg cgaggtgtcg ccgctgatgc tggcattgac
    41701 taccgacgtt ttactctgcc acttatcgtt caacaaggca ggccaggccg cgctggcaga
    41761 cattcgatac ccggcgctca ggctatcacc cagaatcaat aacgtgtccg ctgcggcggc
    41821 acggaaggtt aacaggacca ggaacaggaa gggcaaatgc cagcggaaaa cattgttgaa
    41881 gttcatcatc ttaagaagtc cgtcggtcag ggggagcatg aactctccat cctcaccgga
    41941 gttgagctgg ttgtcaaacg tggcgagacc atcgcactgg tgggcgagtc gggatcgggt
    42001 aagtcaactt tgctggcgat cctcgccggg ctggatgacg gcagcagtgg cgaagtgagt
    42061 ctggtgggac aaccgctaca taatatggac gaagaagcgc gggcaaagtt gcgcgcgaag
    42121 cacgtcggct ttgtttttca gtcatttatg ttaattccta cccttaacgc gctggaaaac
    42181 gtcgagcttc cggcgctgct gcgcggtgag agtagcgcgg aaagtcgtaa cggggcgaaa
    42241 gcgttgctcg aacagttagg gctgggtaaa cgtctggatc atcttccggc acagctttcc
    42301 ggcggtgaac agcaacgagt ggcgctggca cgagccttta acggtcgacc tgatgtgctg
    42361 tttgccgacg aacccaccgg caatcttgac cgacagacgg gcgataaaat tgccgacctg
    42421 ctattttccc tcaaccgtga gcatggcacc acgttgatta tggtgaccca cgacctgcaa
    42481 ctggcggcac gctgcgaccg ctgcttacgg ctggtgaacg ggcaattgca ggaggaagca
    42541 tgattgcacg ttggttctgg cgcgaatggc gctcgccgtc gctattaatt gtctggctgg
    42601 cgctaagcct ggcggtggcc tgcgtgctgg cgctgggcaa tatcagcgat cgcatggaga
    42661 agggcttaag ccagcaaagc cgggagttta tggcgggcga tcgggcgttg cgcagttcac
    42721 gcgaagtgcc acaagcgtgg ctggaggaag cgcaaaagcg cggcctgaaa gtcggcaagc
    42781 agctgacttt cgccacaatg acctttgcag gcgacacacc gcagctggcg aacgtcaaag
    42841 cggtggatga tatctacccg atgtatggcg atctgcaaac taatccccct ggcctgaaac
    42901 cgcaggcggg cagcgtattg ctggccccac gcctgatggc actgcttaac ctgaaaacag
    42961 gcgacaccat cgacgtgggt gatgccacct tgcggattgc cggagaagtg attcaggaac
    43021 cggattccgg ttttaacccc ttccagatgg ctccgcgtct gatgatgaat ctggcggatg
    43081 tcgataaaac cggagccgtg cagccgggga gtcgggtcac ctggcgctat aaattcggcg
    43141 gcagcgagaa ccagctcgac ggctatgaga aatggttgtt accccagctt aaacctgaac
    43201 aacgctggta cggtctggaa caggacgaag gcgcgctggg gcgatcgata gaacgttcgc
    43261 aacagttcct gctgctttcg gcgcttctga ccttgctgct ggcggtggcg gcggttgcgg
    43321 tggcgatgaa tcattactgt cgcagtcgct acgacttggt ggcgatcctc aagacgctgg
    43381 gggcagggcg agcgcaactg cgtaagttaa tcgtcggtca gtggttgatg gtgctggcgc
    43441 tttcagccgt taccggtggt gcgataggcc tgttgttcga aaacgtgttg atggtgctgc
    43501 tcaagcccgt tttgcctgct gcactaccgc cagccagcct ctggccgtgg ttgtgggcgc
    43561 tgggcaccat gacggtcatc tcgctgctgg tggggctacg accgtaccgc ttgttgctgg
    43621 caacgcagcc tttacgcgta ttacgtaatg atgtggtcgc gaacgtctgg ccgctgaagt
    43681 tttatctgcc gattgtcagt gtggtggttg tgctgctgct cgccggatta atgggtggca
    43741 gcatgctgct ttgggcggtg ctggcgggcg cggtagtact ggctttgctg tgcggtgtgc
    43801 tgggctggat gctgctgaat gtacttcgcc gcatgacgct gaaatcgcta cctctgcgcc
    43861 tggcggttag ccgcctgtta cgtcagccgt ggtcaacgtt aagccagctt tcggcatttt
    43921 cgctctcctt tatgctgctg gcgctgttgc tggtattgcg tggcgatctg ctcgaccgct
    43981 ggcagcagca gctacctcca gaaagcccga actacttttt aattaacatc gccacagaac
    44041 aggtagcacc gctaaaagcg ttcctcgcgg aacatcagat agtcccggaa tcgttttatc
    44101 cggtggtgcg ggcgcggctg acggcgatta acgataagcc gacagaaggc aatgaagatg
    44161 aggcgctcaa tcgcgaactc aatcttacct ggcaaaatac gcggcccgat cataatccga
    44221 ttgtcgccgg taactggccg ccaaaagccg atgaagtgtc gatggaagag gggctggcga
    44281 aacgcttaaa cgttgccctc ggtgataccg tgacttttat gggcgatacc caggagttcc
    44341 gcgctaaagt gaccagcctg cgcaaagtgg actgggaaag tctgcggcct aatttctatt
    44401 ttattttccc tgaaggggca ttagacgggc aaccgcagag ctggcttacc agtttccgct
    44461 gggagaatgg caacggcatg ttgacgcaac tcaaccgcca gttccctact attagcctgt
    44521 tagacattgg cgcgatttta aaacaggtcg gtcaggtgct ggagcaggta agtcgggcgc
    44581 tggaggtgat ggtggtgctg gtcaccgcct gcggtatgtt gctgttgctg gcacaggtgc
    44641 aggtgggaat gcgtcagcgt catcaggagc tggtggtgtg gcgcacactc ggtgcgggga
    44701 aaaaactgct gcgtaccacg ttgtggtgtg agttcgccat gcttgggttt gtttctggcc
    44761 tggtagccgc aattggtgcg gaaacggcgc tggcagtgtt gcaggcgaaa gtgtttgatt
    44821 ttccgtggga gccagactgg cgattgtgga ttgtgctgcc gtgcagcggt gcactgctgc
    44881 tgtcgctttt cggcggctgg ctgggtgcgc gactggttaa gggtaaggcg ctgttcaggc
    44941 agtttgcggg atgatgagag taaataaggg gcgttctggt tgaatggaac gccttgttta
    45001 aattaattct a
//
LOCUS       DAZGAP010000012        40590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGAP010000012 DAZGAP010000000
VERSION     DAZGAP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.012"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            40..1806
                     /gene="adeD"
                     /locus_tag="ACHLAM_000597"
     CDS             40..1806
                     /gene="adeD"
                     /locus_tag="ACHLAM_000597"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO7852180.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNTYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTISRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVIRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1853..3244)
                     /gene="uhpT"
                     /locus_tag="ACHLAM_000598"
     CDS             complement(1853..3244)
                     /gene="uhpT"
                     /locus_tag="ACHLAM_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO7852181.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQSFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3500..4819)
                     /gene="uhpC"
                     /locus_tag="ACHLAM_000599"
     CDS             complement(3500..4819)
                     /gene="uhpC"
                     /locus_tag="ACHLAM_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO7852182.1"
                     /translation="MLPFLKAPADAPLLTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGVSALLLLPFLNAQTPREA"
     gene            complement(4829..6331)
                     /gene="uhpB"
                     /locus_tag="ACHLAM_000600"
     CDS             complement(4829..6331)
                     /gene="uhpB"
                     /locus_tag="ACHLAM_000600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO7852183.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6331..6921)
                     /gene="uhpA"
                     /locus_tag="ACHLAM_000601"
     CDS             complement(6331..6921)
                     /gene="uhpA"
                     /locus_tag="ACHLAM_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO7852184.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELVRRMFDGW"
     gene            complement(6994..7284)
                     /gene="ilvN"
                     /locus_tag="ACHLAM_000602"
     CDS             complement(6994..7284)
                     /gene="ilvN"
                     /locus_tag="ACHLAM_000602"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7852185.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7288..8976)
                     /gene="ilvB"
                     /locus_tag="ACHLAM_000603"
     CDS             complement(7288..8976)
                     /gene="ilvB"
                     /locus_tag="ACHLAM_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO7852186.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAIAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9082..9180)
                     /gene="ivbL"
                     /locus_tag="ACHLAM_000604"
     CDS             complement(9082..9180)
                     /gene="ivbL"
                     /locus_tag="ACHLAM_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO7852187.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9744..9833
                     /gene="tisB"
                     /locus_tag="ACHLAM_000605"
     CDS             9744..9833
                     /gene="tisB"
                     /locus_tag="ACHLAM_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO7852188.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9957..10031)
                     /gene="ysdE"
                     /locus_tag="ACHLAM_000606"
     CDS             complement(9957..10031)
                     /gene="ysdE"
                     /locus_tag="ACHLAM_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO7852189.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10113..11297
                     /gene="emrD"
                     /locus_tag="ACHLAM_000607"
     CDS             10113..11297
                     /gene="emrD"
                     /locus_tag="ACHLAM_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO7852190.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11305..11802)
                     /gene="yidF"
                     /locus_tag="ACHLAM_000608"
     CDS             complement(11305..11802)
                     /gene="yidF"
                     /locus_tag="ACHLAM_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO7852191.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11799..12161)
                     /gene="yidG"
                     /locus_tag="ACHLAM_000609"
     CDS             complement(11799..12161)
                     /gene="yidG"
                     /locus_tag="ACHLAM_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO7852192.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12151..12498)
                     /gene="yidH"
                     /locus_tag="ACHLAM_000610"
     CDS             complement(12151..12498)
                     /gene="yidH"
                     /locus_tag="ACHLAM_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO7852193.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(12558..14051)
                     /gene="yidJ"
                     /locus_tag="ACHLAM_000611"
     CDS             complement(12558..14051)
                     /gene="yidJ"
                     /locus_tag="ACHLAM_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7852194.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14048..15763)
                     /gene="yidK"
                     /locus_tag="ACHLAM_000612"
     CDS             complement(14048..15763)
                     /gene="yidK"
                     /locus_tag="ACHLAM_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO7852195.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            15930..16796
                     /gene="yidL"
                     /locus_tag="ACHLAM_000613"
     CDS             15930..16796
                     /gene="yidL"
                     /locus_tag="ACHLAM_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7852196.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(16886..18547)
                     /gene="yidE"
                     /locus_tag="ACHLAM_000614"
     CDS             complement(16886..18547)
                     /gene="yidE"
                     /locus_tag="ACHLAM_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO7852197.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(18743..19171)
                     /gene="ibpB"
                     /locus_tag="ACHLAM_000615"
     CDS             complement(18743..19171)
                     /gene="ibpB"
                     /locus_tag="ACHLAM_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO7852198.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(19283..19696)
                     /gene="ibpA"
                     /locus_tag="ACHLAM_000616"
     CDS             complement(19283..19696)
                     /gene="ibpA"
                     /locus_tag="ACHLAM_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO7852199.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(19690..19762)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20002..20334
                     /gene="yidQ"
                     /locus_tag="ACHLAM_000617"
     CDS             20002..20334
                     /gene="yidQ"
                     /locus_tag="ACHLAM_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7852200.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(20336..21550)
                     /gene="yidR"
                     /locus_tag="ACHLAM_000618"
     CDS             complement(20336..21550)
                     /gene="yidR"
                     /locus_tag="ACHLAM_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO7852201.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            21651..22715
                     /gene="cbrA"
                     /locus_tag="ACHLAM_000619"
     CDS             21651..22715
                     /gene="cbrA"
                     /locus_tag="ACHLAM_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO7852202.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(22712..24049)
                     /gene="dgoT"
                     /locus_tag="ACHLAM_000620"
     CDS             complement(22712..24049)
                     /gene="dgoT"
                     /locus_tag="ACHLAM_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7852203.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(24124..25272)
                     /gene="dgoD"
                     /locus_tag="ACHLAM_000621"
     CDS             complement(24124..25272)
                     /gene="dgoD"
                     /locus_tag="ACHLAM_000621"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO7852204.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(25269..25886)
                     /gene="dgoA"
                     /locus_tag="ACHLAM_000622"
     CDS             complement(25269..25886)
                     /gene="dgoA"
                     /locus_tag="ACHLAM_000622"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO7852205.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(25870..26748)
                     /gene="dgoK"
                     /locus_tag="ACHLAM_000623"
     CDS             complement(25870..26748)
                     /gene="dgoK"
                     /locus_tag="ACHLAM_000623"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO7852206.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(26745..27434)
                     /gene="dgoR"
                     /locus_tag="ACHLAM_000624"
     CDS             complement(26745..27434)
                     /gene="dgoR"
                     /locus_tag="ACHLAM_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO7852207.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            27711..28367
                     /locus_tag="ACHLAM_000625"
     CDS             27711..28367
                     /locus_tag="ACHLAM_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852208.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(28411..29223)
                     /gene="yidA"
                     /locus_tag="ACHLAM_000626"
     CDS             complement(28411..29223)
                     /gene="yidA"
                     /locus_tag="ACHLAM_000626"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO7852209.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTTLSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(29338..29736)
                     /gene="yidB"
                     /locus_tag="ACHLAM_000627"
     CDS             complement(29338..29736)
                     /gene="yidB"
                     /locus_tag="ACHLAM_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO7852210.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            29854..29955
                     /locus_tag="ACHLAM_000628"
                     /pseudo
     CDS             29854..29955
                     /locus_tag="ACHLAM_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29976..32390)
                     /gene="gyrB"
                     /locus_tag="ACHLAM_000629"
     CDS             complement(29976..32390)
                     /gene="gyrB"
                     /locus_tag="ACHLAM_000629"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO7852211.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(32419..33492)
                     /gene="recF"
                     /locus_tag="ACHLAM_000630"
     CDS             complement(32419..33492)
                     /gene="recF"
                     /locus_tag="ACHLAM_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO7852212.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(33492..34592)
                     /gene="dnaN"
                     /locus_tag="ACHLAM_000631"
     CDS             complement(33492..34592)
                     /gene="dnaN"
                     /locus_tag="ACHLAM_000631"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO7852213.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(34597..36000)
                     /gene="dnaA"
                     /locus_tag="ACHLAM_000632"
     CDS             complement(34597..36000)
                     /gene="dnaA"
                     /locus_tag="ACHLAM_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO7852214.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            36297..36377
                     /locus_tag="ACHLAM_000633"
     CDS             36297..36377
                     /locus_tag="ACHLAM_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852215.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            36607..36747
                     /gene="rpmH"
                     /locus_tag="ACHLAM_000634"
     CDS             36607..36747
                     /gene="rpmH"
                     /locus_tag="ACHLAM_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO7852216.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            36764..37123
                     /gene="rnpA"
                     /locus_tag="ACHLAM_000635"
     CDS             36764..37123
                     /gene="rnpA"
                     /locus_tag="ACHLAM_000635"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO7852217.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            37087..37344
                     /gene="yidD"
                     /locus_tag="ACHLAM_000636"
     CDS             37087..37344
                     /gene="yidD"
                     /locus_tag="ACHLAM_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO7852218.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            37347..38993
                     /gene="yidC"
                     /locus_tag="ACHLAM_000637"
     CDS             37347..38993
                     /gene="yidC"
                     /locus_tag="ACHLAM_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO7852219.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            39099..40463
                     /gene="mnmE"
                     /locus_tag="ACHLAM_000638"
     CDS             39099..40463
                     /gene="mnmE"
                     /locus_tag="ACHLAM_000638"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO7852220.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         9765 a        11037 c        10438 g         9350 t
ORIGIN      
        1 aaataataat caaattgata aaatcaaaat gagaaaaata tgaataattc tattaaccat
       61 aaatttcatc acattagccg ggctgaatac caggaattgt tagccgtttc ccgtggagac
      121 gctgttgccg attatattat tgataatgtc tctattctcg acctgatcaa tggcggagaa
      181 atttccggcc caattgtgat taaaggacgt tacattgccg gtgttggcgc agaatacgct
      241 gatgctccgg ctttgcagcg gattgatgcc cgcggcgcaa cggcggtgcc agggtttatt
      301 gatgcgcacc tgcatattga atccagcatg atgacgccgg tcacctttga aaccgctacc
      361 ctgccgcgcg gcctgacgac cgttatttgc gaccctcatg aaatcgtcaa cgtgatgggc
      421 gaagccggtt tcgcctggtt tgcccgctgt gccgaacagg caaggcaaaa ccagtactta
      481 caggtcagct cttgcgtacc cgccctggaa ggctgcgatg ttaacggtgc cagttttacc
      541 cttgaacaga tgctcgcctg gcgggaccat ccgcaggtta ccggccttgc agaaatgatg
      601 gactatcctg gcgtaattag cgggcagaat gcgctgctcg ataaactgga cgcatttcgc
      661 cacctgacgc tggacggtca ctgcccgggt ttgggtggta aagaacttaa cacctatatt
      721 gctgcgggta ttgaaaactg ccacgaaagt tatcagctgg aagaaggacg ccggaaatta
      781 caactcggta tgtcgttgat gatccgcgaa gggtccgcag cccgcaatct caacgcgctg
      841 gcaccgttga tcaacgaatt taacagcccg caatgcatgc tctgtaccga tgaccgtaac
      901 ccgtgggaga tcgcccatga aggacatatc gatgccttaa ttcgccgcct gatcgaacaa
      961 cacaatgtgc cgctgcatgt ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt
     1021 ggtctgaatc acctcggctt actggcaccc ggtaagcagg ccgatatcgt cctgttgagc
     1081 gatgcgcgta aggtcacggt gcaacaggta ctggtgaaag gcgagccgat tgatgcgcaa
     1141 accttacagg cggaagagtc ggcgagactg gcacaatccg ccccgccata tggcaatacc
     1201 atttcccgcc agccagtttc cgccagcgac tttgccctgc aatttacccc cggaaaacgc
     1261 tatcgcgtta ttgaggtcat ccataacgaa ttgattaccc actcccgctc cagcgtctac
     1321 agcgaaaatg gttttgatcg cgatgatgtg tgctttattg ccgtacttga gcgttacggg
     1381 caacggctgg ctccggcctg tggtttgctc ggcggctttg gcctgaatga aggtgcgctg
     1441 gcggcgacgg tcagccatga cagccataat attgtggtga tcggtcgtag cgcagaagag
     1501 atggcgctgg cggtcaatca ggtgattcag gatggcggcg ggctgtgcgt gatccgtaac
     1561 ggtcaggtac aaagtcatct accgttgccc attgccgggc taatgagcac cgacacggcg
     1621 cagtcactgg cggagcaaat tgacgccttg aaagccgccg cccgtgaatg cggtccgtta
     1681 ccggatgagc cgtttattca gatggcgttt ctttccctgc cagtgatccc cgcgctgaaa
     1741 ctaaccagtc aggggctgtt tgatggcgag aagtttgcct tcactacact ggaagtcacg
     1801 gaataataaa aaaaagcccg gcgtcatgcc gggcaaaagt caccagttaa gtttatgcca
     1861 ctgtcaactg ctgaattttt ttctcgcggc ggattttgcg ttcttccatc accgccacta
     1921 tcgccatcag gcagatacaa ccaatcgcgg cgatatccag cgcggcgaag gtgcctgccc
     1981 agccggtaag gccaaatacc ggcgtcccat cggcaatcat tcccagacct aacttggcaa
     2041 agctgtcacc aatcaggtaa gcgaaggtgc ctttaatacc atcggcagcg ccaatcgctt
     2101 ttttaggtac aaagccaaca gcagccacac caatcaacaa ttgcgggcca aagaccagga
     2161 aacccaacgc aaagagagaa gccaggtaga tatattcgtt gctggcgtgt tgatacacac
     2221 cgagcgtggc gataatcagc gccagcgcga tacaggccac caggccacgg cgaccgttcg
     2281 ccaggtcaga gagccagccc cacagcagcg taccgaccag cgcaccagct tcaaacagcg
     2341 taaagctctg aatcgccacc gctttagaga gtttcagttc ctggaacgca tacacggttg
     2401 accactggtc gataccaata cgcaccacat agaggaaaat gttggcgaag cacagcagcc
     2461 agatcacttt gtttttcagc acatactcaa caaagatctg ccacttggtc atatcggtag
     2521 attctgtctc tttgtcctct tcgctgatct cctcgccgaa cagctcttca gctttgccga
     2581 ggccataaga ttccggggag tcgctgccgt aacgcaggcc gataaaaccg acaatcagcg
     2641 cgataatcga cgggaaaata aacatgccga tgacatgacc atcgaacagg taatttgcgc
     2701 cgaacagcgc cacacctgct gcgcctgcac caccaaggtt gtgagaaatg ttccagaaac
     2761 cgaggaatgt cccgcgttta cgacgcggcg tccatttggt gatggtggag taactgcacg
     2821 aaccgccggt actctggaaa aagccgctca aggcgtagaa ggcaatcatc aggaacaggc
     2881 taaccgagcc gctgcccata ctggcactga agcccagcat acaaatggcg gacaggatca
     2941 gcatgaacgg caggaattgt ttggtgtttt tgccgtcggc gtagtaagaa accagcgttt
     3001 tacccacgcc ataagtgatg gagaagccca ggccgatcat ccccagctgc gtcatgctca
     3061 acccgtaggt cgagatcatg tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca
     3121 tcgtaaggta gccgataaag accaccaggt aggattgcat gaacggtttg aaccacattt
     3181 tgcgccgcac ttcgagcgga aggtccaggg tcggcttgcg aacctggttt aggaaagcca
     3241 gcatgggtta ctcctgattt cccttcatac ttcaagccgc aggtgcctta gctgcacgca
     3301 ttcctcccgg tcacatagtt cactatgctc ccgggactca cgcgtttgct gcgttcctgc
     3361 aacttgaatt attttgggaa agaattaatg aatgatggca ttgtaaaaat cagcagcaaa
     3421 tcaggcctga gatagcgtcc aggcaaaacc tggaaaattg cttagttttg ccggatatga
     3481 agtgaaaagg tgagatgcat cacgcttcgc gcggtgtctg ggcgttcaaa aagggcagta
     3541 acagcagtgc ggaaaccccg gcggcaatgg cgataaccac aaaaaatccg ctccagtgcc
     3601 aggtatcgag cactttcgcc agcggccaac cagcaagcga cgcccccaga taagcaaaca
     3661 agccgacaaa ccccgtcgcc gcccctgccg cctctttgtg ggaacattct gccgccgcca
     3721 tgccgattaa catctgcggg ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct
     3781 gcatcacgta gctggcaaat ggcatcagcc acagcgagcc gactgaaagc aaaattccgg
     3841 cggcgaaaat caaattcatc ggcccgcggt tgccgttaaa caatttgtcc gagccccaac
     3901 ccgctaccag cgcaccgata aatccaccca gttcaaacat cgtcactgcc gtattcgccg
     3961 tgaccagatc gacgcccagc gtctcggaca tatacaaatt gccccagtcg ttgattgccg
     4021 cccggaccac atagaccagc acatagcaaa acgaaagcag ccagatatac ggattcagta
     4081 acacatattt ggtgaggatc tctttacgcg tcagccctgc cccttcttgt tgttgagcta
     4141 tctccagcgc gtcgtgccgc cattcaccaa ccgccggtaa acctaacgcc tgcgggcgat
     4201 cgcgcagccg ccagcagaga aaaatcccca cgactatcgc catacaaccc gcaatcatca
     4261 tcccggcacg ccagccgtaa tgcagcgcgc tcgctgccat cacaatggga atgagtgcgc
     4321 cgccgacgtt atgcgccgtg ttccataatg cccaccaacc gccgcgctcg gtacgagaat
     4381 accaggcggt taacagacgc gcacacaccg gtgaacccca gccctggaaa aaggcgttca
     4441 acacccaaag cacggcaaac gcccatagcg acgtcgagaa gccaaacaga atgttgataa
     4501 tgcccgtggc aataagccct atccccataa aataacgggc atttgagcga tcgctgacaa
     4561 tgccggagac aaacttcgac acgccgtagg taatgtaaaa cagggtcgct aacaggccaa
     4621 tatcgctacg gctgagcacg ccgttagcaa ggatttctgg tacggcggcg ttaaaacttt
     4681 tacgcgtgaa gtaaaacagc gcgtaaccca gccagatggt cagcagaata tgccgacgcc
     4741 agtagcgata gcgggcatca atttcatgtt tatcagtcag taatggcgca tcggcaggcg
     4801 ctttcagaaa cggcaacatc atcgaacctt agacataacg ttgaggtaga gaaacgctga
     4861 cacgcgtgcc gtgcagacag gaaatgtgta atgtgccacc cagcgccgtt acgcgctcgc
     4921 gcattccggt aaggccaaaa ccgtgttgcc cggaatctgg cggtaaaccg ctgccatcgt
     4981 cttcaatcac cagcattaac cgctcatcat gctgccagcc ttgcagcgtg accgcgctgg
     5041 catcggcatg tttcacaatg ttgttcaacc cttcctggca gacgcgaaat agcgtcacgc
     5101 gctggttttc gcttaacgct gattcatcga ttcgccattc gagatgactg acaatgccgc
     5161 gcccttccag ctccatttcc cgcatcagtg agcggatggc ctgctccagg gtgagatcat
     5221 ccagctggcg cggacgtaac cgccctaaca agcggcgcac cgcgtcgtaa acgcccagcg
     5281 atagttgttc gatgagctgc ccgctctgtt tcacgctggc gttatccgcc gcgagccgct
     5341 ggacaatgcc cgcttgagtt cgaatagcag taatggtctg accgatatca tcgtgcagct
     5401 cacgtgccac atcacggcgc acgctctctt cggtttccag caaccgttca gcgagatgct
     5461 gattgcgcgc cagttccttt tgcagcgact ggttaagttc acgcaaccgc tggatgcctg
     5521 cgcccagtaa caaccctgtc agactttgca ccagcagcga gagcaataaa tccaccggat
     5581 gatcgcgcca ggtttgactg gcgatcagcg cgatggcgtt catcaacgtc gcaattagcg
     5641 ccccttgcca gccatagtgc caggccagcg cgataatcgg cagcgccagg cagaatggcg
     5701 taaagcgcga cagttcgtcc ggcaatccca actggagcca gagactgata acaaacagca
     5761 gcaagtacca gaccagatgc cgcccacgcc agttgatcgg ttgagaaact aacgacgggc
     5821 cgagcggcag ccaggtgtta ttggcgagat agtgccagaa caccagacat atcggggcca
     5881 gcgtcaggcc gccagttaaa gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa
     5941 gccagggcag cgactgcaac aacgccgccg ccgttaatgc cgccccttgt agcagcaagg
     6001 tgcgccagtc acgttgatgt cgatagcgcg agatcagcgc cacgggcagt aacgtcagta
     6061 aactaccgat catcaataag gagaaatggg ttaaaccgac cgcctgcgtt aaccagtaaa
     6121 tcagcagcca ctccgcgccc agcaacaccg gccagtatcc gcgcgggcat tgcagcatta
     6181 gccccagacg cagaccaaac ggaaataaca gcaccgccat atcagggcgc tcaaccaggt
     6241 gcaggctgat actccacagg caaaaccatg cggcagagaa gataaaaaag caggcaataa
     6301 cggtaattaa gcgggagaac aacgtcttca tcaccaacca tcaaacatgc ggcgcaccag
     6361 ttcaacgtcg ttactgacgc ccagtttttc catcagattg gcgcgatgga cgtgtaccgt
     6421 tttcggcgac aagcccagtt cggcggcaat ctctttcacc gccattcctt gcgccagttt
     6481 ttctgccacc tggcgttcgc gtttggttag cgggtcctga cgaccggatg ccagtttaat
     6541 ggcaatatcc ggcgtcagat aacagccgcc cgtggcaacc gtatgcaccg cagcaatcag
     6601 ttcgtcaggg ctacaacgct tggagagaaa gccgcgcgcc cccgcgttaa gcgcctgctc
     6661 aaccagcgcc ggactgtcat gaacagagag cataatcgtc gccatacctt tcggcagctg
     6721 gcttagcagc tccagaccgg agatatcggg catggagata tcgcaaatac acacctgcac
     6781 accgcgcccc ggcaaccccg ccagcgcctc gcgccccgaa ccaaactcgg caactacctg
     6841 caaatcaggt tccagcccca gcagctgcgc aaagccggag cggacgataa ggtgatcgtc
     6901 tataagggca acggtgatca tgatcttgtc ctggcgggta ataaaacgcg cttaccttaa
     6961 cgataagcgc gatgttgttc aagccttgag cggttactga aaaaacaccg caatcttgtt
     7021 aaacattgtc ggatcggact gattacgctg cactttcacc acatcttcca gcttatcgat
     7081 ttggcttatc atctgctcca gacgctggtc gtcattgacc agtagccaga tatggctttt
     7141 gtcgctgtcc tgaatcggca ggcagagaat gccttcaacg ttaaaagcgc gacgggcaaa
     7201 aaggccacaa acgtgggtca ttacgcccgg atggttgcga acggtgagtt cgagaatcac
     7261 gttgtcatga gttgtgtttt gcatggctta ttcccccacc atttcagtat tcgccgcacc
     7321 tggcggcacc atcgggtaca ctttttcttc ggcatcaatg cgcacgtgga tcagcgccgg
     7381 accagggcga ttgatgattt cctgcaatgc agcctgcgga tcggtttcgt tattcaaatc
     7441 acaggtttcg aggccgaatc cggcggcaat ctgcataaag ttgatttttc ccggataggt
     7501 agcggcaaaa acgccctgct tgtagaacag actctgttgc tgatgtacca gccccagcgc
     7561 ttcgttgttc atcagaatga ttttgacatc cagctgattt tcgctagcgg tcgccatctc
     7621 ctgaatattc atcatcaggc tgccgtcgcc ggagaaacac aacactttgc gatccgggtt
     7681 cgcaagcgct gcgccaatcg ccgcaggcag gccaaagccc atcgtgccca gcccaccgga
     7741 ggtcagccac tggcgtgggc gattgagcgg ataagcctgc gccgtccaca tctgatgctg
     7801 acccacgtcg gtggtgataa tcgcgctgtc atcgacacag gcggcaacag cgttgatcag
     7861 gccgtaatgc gttaacggat cgcacgcttt cgggattgga cacgggaatt cacgctgcaa
     7921 atccgccacc aactggtgcc actctgcacg cggttgcgct tccaccagcg ggatcaactg
     7981 cgccaacacg tcatcaacat ccgcctgaat tgccacatgc ggttgcttga ttttgcccag
     8041 ctctgcgcgg tcgatatcga catgaataat tttggcattc gggcagaact gctcggtttt
     8101 gccaatcgcc cggtcatcaa aacgcgcgcc gagaacaatc agcagatccg cctcctgcaa
     8161 aatatagttg gtgctgcgca cgccgtgcat ccctagcata cccagcgaca gcggatgcgc
     8221 ttttggcaac atacccagcg ccattaatgt cattgtggta ggcagctgcg ctttctccgc
     8281 cagttcacgc actctcgcag gcgcattgat cacaccaccg cccagataaa gcaccgggcg
     8341 tttggcagca ttaatcattg ccgctgcgtc acgaatgctt tcttcgctaa aggcgggcgc
     8401 gatggctttt tctgccaccg cgggctgtgc ttcaatctca aaaacggccg tttgcacatc
     8461 cttaggaatg tctatccaca ccgggccagg gcggcctgat tgcgcaatac ggaaggcatc
     8521 gctcatgacc tgcgggagtt cttcgatatg tctgaccaga tagttgtgtt tggtgatggg
     8581 gatagagatg ccgtaggtgt cgacttcctg gaaggcgtcg gtgccgatca tcgaggcggg
     8641 aacctgacca gtgatgcaaa tcagcgggat ggagtccagc cgcgcatcgg caatggcggt
     8701 caccaggtta gtcgcccccg gtccgctaca ggccatacag accgccggtt taccgtcggt
     8761 gcgcgccatt ccctgagcga taaaccccgc gccctgttca tggcgagcca gaatatggcg
     8821 gatttgcgtg ctttggctta aggcatcgta aacaggcagg atagaaccgc ccggaatgcc
     8881 cgtcacaatc ttaatgccct gctgttccag gaaatgaacg ataaattctg cgccggtaaa
     8941 gcgcttacgc gtcgatgttg tgcccgaact tgccatgctc cagtcctttt cttctgggcc
     9001 gactttccgg gaggtgctta aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg
     9061 aatcgtgtgt tgttccagtc cctacggcgc attgccgacg accaccacca cacgcacgac
     9121 gaccactgcg gcggatggcg cagttggtag tagttttgcg ttgagcatgg aagtagtcat
     9181 tggggacctt gttggttttg tgtttaacaa tatttataca agcacagctt tacaggggag
     9241 acaatggaaa atttttcagc aagggaaaat tgaggggttg atcacgtttt gtactgaatt
     9301 gcaggtaaca aaaaaccccg ccggagcgag gtttcgtcag tcgcctgcgg ctggtaaccg
     9361 caaagcacac tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat
     9421 ttcggcacga attttgacgt atttagtgca tagttgagta tcgatcacag tttgcgtttt
     9481 gtccaaaatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
     9541 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gcagaaacgg gtggtgccgt
     9601 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
     9661 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
     9721 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
     9781 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
     9841 tcaagtcgca ctaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
     9901 gtgtcggatg cgatgctgac gcatcttatc cgccctacca tctctcccgg caacatttat
     9961 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    10021 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    10081 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcatagaaac gtcaatttgt
    10141 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    10201 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    10261 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgatt tccgaccgcg
    10321 tgggtcgccg accggtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    10381 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    10441 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgcga tttatatgaa cggacacagt
    10501 tgcgccatgc taacagcctg ttaaacatgg ggattcttgt cagtccgttg ctcgcaccgc
    10561 taatcggcgg tctgctggat acgatgtgga actggcgtgc ctgttatctc tttttgttgg
    10621 ttctttgtgc cggtgtgact ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    10681 acgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    10741 actgttattt gctgatgctg attggcggtc tggccggaat tgccgccttt gaagcctgct
    10801 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    10861 ttctgccgat tccggcggca ttttttggcg catggtttgc cggacgtccc aataaacgct
    10921 tctcaacgtt gatgtggcag tcggttatct gttgcctgct ggctggcttg ctgatgtgga
    10981 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cattcccgcc gcgctgttct
    11041 ttttcggtgc cgggatgctg tttccactgg ctaccagcgg cgcgatggag ccgttcccct
    11101 tcctggcggg caccgctggc gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    11161 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctggga ttgttgatga
    11221 ccttaatggg attgttgatc gtgctgtgct ggctaccgct ggcgacgcgg atgtcgcatc
    11281 aggggcagcc tgtttaagct cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    11341 atgctgcttt atcagatcac gcatcacccg catatgcggc gcggagtaag aataaaacgc
    11401 ctgatagccc gcacaaagca cgttgtctgg tttatcttcc cgagcgtgcg ggcacagccg
    11461 atgccagtcg cacgcctggc agtcacggcg cggttgcgac ttttcgctgc cacaccattt
    11521 ttgtaaggtg gtgtcaaaaa gttcaatcga aatacgactg atatctgccc gcacccaaca
    11581 ctcgaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    11641 atcgccccgg tcatcaaatt caagatgggg aataagctgt actgagcgga tgcccagctg
    11701 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttatactcta caaccagttt
    11761 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    11821 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    11881 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    11941 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    12001 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    12061 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgttc cggctgcagc
    12121 cctgggtcgg caatgcgtct ggctttgcgg ctatccggca tacaacacca gtcccatgac
    12181 gatcactgcc acgaccatca atattaaact gatgattaat aagctgttgg tatagggcag
    12241 gtcctctttc aggcgcatcg ctttttcatt gcgtaaccag cgcaagtagc catacatcgc
    12301 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcgcggatga cgggcgtggc
    12361 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcagtacg
    12421 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    12481 gaggcgggaa attttcattg ttgctccttg taaactgttc ctcgccgaaa aattaggcca
    12541 ggaacagcaa taatgcatca gaacttctgt tttttctctt ccaccttcac cccttgtgtc
    12601 ggtaaaccgg tgtcatagtc gcgtaccact ggagaataac catcctgtgg gcgtggacga
    12661 aacgccccca tccagcgcgg ttgtgcatct ttgcgccacg gacgcaggtt ccattgataa
    12721 ctgcggaacg gatcgcgaat tttgtccata taatccaata aggcgtcatg cattttgctg
    12781 cgaacgtcgg caaaatggat atcatcgatc aggttatgca tttcatttgg gtcattacgg
    12841 cgatcgtaaa gttcatcact ggtgaagagg ttgagcacca gtttaaagtc atccgttacc
    12901 cagcaacgca ccggaataaa accgccaaag ctgtcatgct caatctcgta gcgattaaat
    12961 tccaccaaca cgccacgtgg ttcttttacg gcaaggatat tatcccccgg cagaatctct
    13021 ggtttttcaa tatctgccag cgccatcatt gtcggcagta agtcaatatg actgactggc
    13081 gtatcgacct gtcgccgctc cccttgcggc gaacggatga tcagcggaat gcgggtgatg
    13141 tcgtcataca tcgccgcccc tttactgatc agcttatgtg cgcccatcat ttcaccatga
    13201 tcggaggtat aaatgaccca cgtattttca cgttgctctg gcgttaaggc attgatgaca
    13261 cgtccgattt ggtcatcaac aaagtcattg caggcaaaat agagcggatg gtgataaagc
    13321 ccgtcatcac cgactggcga tggcatcgcc tgcgcccata agcggtgatg ttccggtttg
    13381 ttcgccaggt catcctgtga tttctcgcca agttcgtagt aaaaatcagc gtatttctct
    13441 aaatactcca ccggacaggt gaacgggtga tgcggctcat catacgaaac caccatcagg
    13501 aaaggttcgt cggcgcgcgc gggctgttgc agaaaatcta ccgcccgatt gctgatgcgg
    13561 tgcgcccagg tgaaggtttc gtcgatatgg ttcgcctgta aatcctcaac gctgtttagg
    13621 ccattgcgcc acaggctgat ctctttttcc gtcagttcgc taaggtagtt cgccccatcg
    13681 aaccagtaat cagcgtccca ctccggcgga cactcgccag tgccgaaata gtcatgaccg
    13741 tcgagatgcc atttgccaat gtaacaggtg tgatagcccg catccttaaa gtagcgcccc
    13801 atggtggaaa tgtttttacc tggcgcgacg ttgttggtcc acgggccgga ctggttagcg
    13861 tagataccgg taaacagccc ggcgcgcgct ggtgtacaaa ccggtgaaca ggtataggcg
    13921 gaattaaagc gaatactttc cgccgccaga ctatcaatat tttgcgtatt cagcggctta
    13981 ccgctatagc aaccgaccat attggtggcc tgggtatcgg tcatgatgaa cagaaaattg
    14041 gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat
    14101 gccgccagct atcaaaaatc aggtagacaa tcactacggc ggcaatgaaa taactgatca
    14161 tcgctaacca gcgcgtaccg tagccgccga attcagccag cccggcatag acgccaatca
    14221 tcgcgaacag gatgcccatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg
    14281 caaacgcatc tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca
    14341 tcacgaccac gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact
    14401 tcaccagata gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg
    14461 ccagcgccgg gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt
    14521 tgtaaatgcc gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc
    14581 acggcgctac cagcaccgaa acgatggcga taaagaaacc aaatttgcgc ccgacggtga
    14641 ccagctgctg cggctcggca ttctggttaa tgatgcgccg gtaaataccc atactgaata
    14701 acgtactggc gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg
    14761 cgccgaagaa acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca
    14821 tgtcggcttt cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta
    14881 ccagcgggtc gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca
    14941 ggctttttga cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt
    15001 tcaccagaat caaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccgccga
    15061 ctgagtttaa tttctcggcg tgaacggtgg tgagttgctc aatgccctgc ataaagctgc
    15121 ccttgcccat cgcgatcagg ccaaataccg gcaccatcaa cccgccaata accagcccaa
    15181 taccgttaat ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa
    15241 ttcccgccag accaagcaaa ataaccagta accagatagc cgcaccatcg gaaagttgta
    15301 gcgactcacc cacatgaaac aggctgttga gcgccaacgc gccggaatag agaacaatcg
    15361 gcagaaagca gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt
    15421 tatcataacg ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg
    15481 gtaaaaagat tagcgcgagg aagatcagcg tcactgcaga ggtgacttcc cagcccatca
    15541 ccgacatgcc acttttgtag gcctgcccag aaaggccgac cagttgttcc gtggaaaggt
    15601 tggttagcat taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat
    15661 agccttgttg cgatccggta tctgttttac ggaccttcca ccaggtgatg accgctacca
    15721 gcagcgtaaa accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta
    15781 tgtttatgtc ccggacgcat gaatataggg agaggtaaaa tgcgtcgggt caggcagtca
    15841 gattcttaaa atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg
    15901 tgagcataat tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcggatgtaa
    15961 aaaacgaaac tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa
    16021 tttctctgat atcgctctgg catacctacg ccgacgagca ttatcgggtg atctggccgc
    16081 gggacaagaa gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat
    16141 gtgggaaaat tcttttaaag aatgacgaac aaataactct gcatggcaac tgtattatat
    16201 ttttaaagcc aatggatatt cactcttatc actgtgaagg tttggtctgg gaacagtatt
    16261 ggatggaatt tacccccacc agtatgatgg atattcccgt cggtcagcaa agcgttattt
    16321 ataatggcga agtttataat caggaactca ccgaagttgc tgagttaatt acttcaccag
    16381 aagcaataaa aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt
    16441 gtcttatgca cgcagtcggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg
    16501 ccactttaca tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc
    16561 cctgtagcga ggcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag
    16621 aatattacct cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caagggaact
    16681 cggttggcga agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct
    16741 ttaagcataa atttggttat gcgccgtcag ctgtgctgaa gaatacggac aggtaataac
    16801 cagaatggcg agtgtgtaaa tgactcgatg atgttcagaa ataccaggtg gagtaccatt
    16861 tacggcgact ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt
    16921 ggggggtgat aatgcgcagg aacatcacca acggatagac agtggcgtaa gagagcgccg
    16981 ccgcaccgct cgttggatga agattattag caaatgccag cgccggagga tcggtcatgg
    17041 agccagccag catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa
    17101 tgccaacagt aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc
    17161 cttcgccatc caccagtgta tggataaaat cgccgccgga tttcaggcca accaccgaga
    17221 gaaagagcac aatgcctaac tcgcgtagcg ccaggttagc gcttggtggc ataaaccagt
    17281 acagcttgcc gatactgccg atacgcccga ggatcaacgc cataatcagc ggaccgcccg
    17341 ccagcccgag tttcaacgcc gccgggaacc caggcacaaa gacgggaata gaacctaaca
    17401 atacgccaag cccgatacca ataaacactg gcagcatctg aacctgttgc agtttttgct
    17461 gcgcattccc cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga
    17521 ggatatcgcc gaactgcagg ctgatatcgc tacttgcaac taattcaaca ccagcacggt
    17581 tcaggcgcga gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgtttac
    17641 cgagcacgtt ttcattggtg accaccacac gctcgacgcg caagtcagtg cctttcgtag
    17701 acagcgaggt atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgcaggct
    17761 gccccaccag atgcagcaaa tcgcccagtt gaataacggt ttctggcgaa ggcactttta
    17821 gggtttcttc tcgtttcaat cgtgagcaaa taactttatc gccattgaga atcggcacat
    17881 ctttaatggc taaattatgc aaattaggat tctcaactcg gatgttaatc gtcctgatta
    17941 gcgcaccgcc gttggttcgc gtggactcgt gctgctgggc ctcagtctca acattgacgc
    18001 ggaaaataac ccgcagcatc cacatagtga acaaaatgcc gcaaatccca aatggatacg
    18061 ccatcgcata gctcatcccc atttgatcga ccatcgccat cggtgtcccc aggtcgcgca
    18121 agatctgctg ccctgccccc agtgctggcg tattggttac cgcaccggag aaaatcccca
    18181 gtactaccgg cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agccccccga
    18241 tgatgacgat cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa
    18301 agaagcccgg ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct
    18361 gaataacatg cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga
    18421 cgatgatccc accgaacagc acgccgccaa tgcccaatcc aacaccgcga aacttgacgt
    18481 tgccgataaa caacccgacg accgccacca aagccagaat actgaccgtt aatgctatat
    18541 cactcatcgt ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg
    18601 gtgaagcaga tttgccttat agcgcacatt atcacgggaa agaacgtgcc gaaatatctt
    18661 aaacaggcga aaattaacgc attgttgaag aagaaagaca aaaaaagccc caccagaatg
    18721 gcggggcaaa gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac
    18781 gctgcgctgc gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta
    18841 cgaaggttgc accagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct
    18901 gattcataag cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt
    18961 ttacgctcag gcgcgtacct tccagttgaa tctctaaatc ttcctgacga aaacctgcca
    19021 gcgcaagggt aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc
    19081 tctggctttc accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac
    19141 gcatcagtgg ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa
    19201 gtacctgcaa atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc
    19261 cgcgcaaggc ggccttaggg aattagttga tttcgatacg gcgcggtttt ttcgcttccg
    19321 gaatcacgcg ttcgagatcg atatacagca aaccatttac caggttagca ccacgaacat
    19381 gaatgttctc agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca
    19441 gataggtgcg ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct
    19501 gggcggtaat ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat
    19561 ggttttcgtc taccagttca acgttatacg gagggtagcc gccattactc tggctctggt
    19621 tgttttctaa gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata
    19681 aatcaaagtt acgcataatc aatagctcct gaaatcagcg agaatctaag accttccaca
    19741 atggacaggt caggtagcca gaacacccat caggcgcgtc ctcatcggct acgatgtaaa
    19801 aatgggtctg gaaatgaact tttcaagcct tatcagcgga cttttttttg cagtttatgg
    19861 tctattgcat agactgaggg ggcagcacga aacgttaaag tgcgatagcc gccacagtgc
    19921 gacgtaatga tgatgttatt ttcagcaagg gatcgctata actcatcagg ccaacaccca
    19981 cgatcaacag atgaatttat tatgataaga aatgtgttgt tagcgttcat gatatgcagc
    20041 ggaatgacat tacttggagg atgctccagc gtgatgtccc ataccggggg gaaagaagga
    20101 acgtatcccg gcacccgcgc cagcgctacc atgattggcg acgatgaaac caactggggc
    20161 actaagtcgc tggcgattct cgatatgccg tttaccgctg tgatggacac gcttttactg
    20221 ccgtgggacg tgttccgcaa agacagctcg gtgcgctcac gcgttgaaaa aagcgaagct
    20281 aacgcgcaag ccaccaatgc ggttattcca cccgccagaa tgcctgataa ctgatttagc
    20341 gatcagtttc ggtgatccac agctggccgc cttccatcca cgccagccac tgaccatccg
    20401 gcgagaagac cacggcgtcc gcagaaggtg gattggcgtg gttttcggtt aaatactcaa
    20461 cctcgccgct ttgcgcatgg gcgcaagcaa ttcgattatc cagcacaaag cccaaccatt
    20521 ctcctgatgg atgccagtta aatgcagact gaatatccgt tttgttatgg gttaactggc
    20581 gcggctcgcc gccctgtggc gagataagcc acagttgcac aatgccgtta tcatcacgca
    20641 ttaaaaacgc gatttgcgta ccctgcggat tacagcgcac ccagtggcgc gggacgttga
    20701 ctaaccccgg ataagcccga tggtgggtaa aggttaaacg tcgctgcacg acgccacgcg
    20761 gtggcgcggg cagggttgtt tccgttccac ttaacggcgc atcaccagct gctttccagc
    20821 cagcttcatc ttgcggtaac tcaacgataa atagctccgg cactttctcg ccctttggcg
    20881 aaagtgtgtc gccaataaac gccagcgcgt gatttcctac ccatccttct tcataagcac
    20941 gattgatttc attactgcca ggctgcggcg tgggcgttgt tttactcacc agcacgcacc
    21001 agtggctacc gctgtattca cgcggatgct gcttttgtac gttgaccggg ccaaacggcg
    21061 cagcaacacc tacgtttcgc aaatccagcg tcgaatcgag ttgatgcatt acatggtcgt
    21121 tataggtaaa gctcaccctt tctccgttcg ggctaaagac atgcacatgg ctgccgccgc
    21181 gcagcgctcc tggggtgtac ggcgcggtaa tatccattgc atcgagattg ctcaccttgc
    21241 cgccttcagc aatcactccg cgccgatgat ggaagtcgta ataccatgtt tcatcaggat
    21301 tttccgggcc gtggatgaaa acatatttct ctgacttcgg atgaacggtc accacgccga
    21361 cgtacgcgcc ctgtgacgcg cgatagataa cctcgacctc gccggtatgg atattcacac
    21421 gctcaatggt ctcgccggta aacgacgccc ctgaaggacg cacgtcaaat accagccact
    21481 ggctatcggg cgtccaggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta
    21541 tctgtttcat ggcgcggtcc tgatacaaaa cgggtcccgc catcatactt cacaaggact
    21601 tcactttcag acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt
    21661 tcgacgtggc gattattggc ctcggcccgg cagggtcggc gttggcgcga aagttagcag
    21721 gcaaaatgca ggtgatcgcg ctggataaaa agcaccagca tggtactgaa ggtttcagca
    21781 aaccttgtgg cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatggactga
    21841 cgcttcctgt cgatgtcatc gccaacccgc agattttcag cgtcaaaacc gtcgacgtcg
    21901 ccgcatcgct cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg
    21961 acttgtggat gaaatcgctg atccccgcca gcgttgaggt ttaccacgac agcctgtgtc
    22021 gaaaaatctg gcgtgaggat gataaatggc atgtcatttt tcgcgcagac ggctgggagc
    22081 agcatattac cgcccgctat ctggtcggtg ccgatggtgc caactcgatg gtgcggcgac
    22141 atctctaccc ggatcatcaa attcgtaaat atgtcgctat ccagcagtgg ttcgcagaga
    22201 aacatccggt gccgttctac tcctgcatct ttgataatgc gataactgac tgttattcat
    22261 ggagtatcag caaagacggt tattttatct ttggcggtgc ctatcccatg aaagacggtc
    22321 agacgcgttt cacgatgctg aaagagaaaa tgagcgcctt tcagttccag tttggtaagg
    22381 cggtgaaaag cgaaaaatgc acggtgctgt ttccctcacg ctggcaggat tttgtctgcg
    22441 gtaaggacaa tgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg
    22501 aggggattag ctatgcgctg gatagcgcag agattctgcg ttcggtgtta ctgaagcagc
    22561 cggagaagag caacgccgcc tactggcgcg ccacccgcaa actgcgttta aaactcttcg
    22621 gcaagatagt aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca
    22681 gtggtgtggc gcatattcca cagttgaaag attagccaac gcgcttcaca tcgcccacca
    22741 gcaggatgta agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggtg
    22801 cgaaaccgta accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac
    22861 caccggcgaa gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag
    22921 agaccagcga ccaggtaatc gaagcaaaac cgttgccgaa gaatgccagc gccatcaggc
    22981 acataatcat catcggatcg ttggtgtagt tagcgcccat aatgcaggtg gagatcagca
    23041 agccgcagat aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca
    23101 gatccgcgac ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg
    23161 tcataaagcc tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc
    23221 aagttaagaa aaaccacagt gtagaagcca ccgcaaattg cccaagatag acgccgatca
    23281 gtttacgatg gaacaccagt ttccagtctt tggctgttaa cggctgacgc gcctcttttt
    23341 tcaccggcgc atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt
    23401 tgctgatgcc tttagtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga
    23461 taatgccgat accaccggtg acaatgaaca cccagtgcca gctcaacatt tcctgaatcc
    23521 agatcagcag cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa
    23581 cggcggaagc gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga
    23641 aagcgggcgc ttcgaaaata ccggttattg cgcgcagacc aattaatgac atcaatcccg
    23701 tggcaaagcc ctggaataaa gtcgccactg accagccaaa tatcgcaata aaataagtca
    23761 cgcgtgaacc tacgcgatct aaaaaccaac cgccggggat ctgacatagt gtataaagcc
    23821 aggcgaaggc cgaaaataca tagcccattt ccgctttggt aataccgaac tcttcctgaa
    23881 tatgggcgga agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa
    23941 agatcatcac cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat
    24001 ccatagcgag ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt
    24061 tacagagggt tatatttttg tattgctgtt ttagtgcccg atgagaggct tacgtggcag
    24121 gaattaccac tctgctacgc tgttatcttc atgacgccag agcggattac gccagtccgg
    24181 ggcattttta ctgaactcaa taactttagc ttcgtcgatt tccacaccta agcccggttt
    24241 cgttaatggt ttaaagaaac cgccgaccat gctgaagtct tctttgtttt tcacaaagtc
    24301 gagtaactcc gcgcctttgt tgtaatgaat gcccatactt tgttcctgaa gtacggcgtt
    24361 ataggaaaca aagtcgatat gcaggcaagc cgccagtgca atcggtccga gcggacagtg
    24421 cggcgcaagg gtcacgtcat aggcttctgc cattccggca attttgtagc attcggtaat
    24481 accgcccgcg tgggagagat ctggttgcag aatcgaaata ccacccgcct ccagcacacg
    24541 tttaaaatcg aaacgtgaga acatgcgttc acccgccgcc agtggaatat gcgtttgtgc
    24601 cgccagtttc gggtagtatt cggcttgttc tgccagcacc ggctcttcaa taaacagcgg
    24661 gcgatacggc tccagttctt taatcagcac tttcgccatc ggcgcgctga cgcgaccgtg
    24721 gaaatcaaga ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt
    24781 gactgccgca tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag
    24841 tttgaaggta tcgaagccga tttcgcgcag ggttttaatg ccgtcgataa catccgccgg
    24901 acgatcgccg ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag
    24961 ttgccagacc ggcgcattca gcactttgcc tttgatatcc cataacgcct ggtcgatccc
    25021 ggcgatggcg ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg
    25081 ccataagtca ttgatgcgcg atggatcctg accaatcaaa tagtcaccca gctcgtgaac
    25141 tgccgcttcc accgtgcggg cgcggccttc aatcactggc tcgccccagc cgaccacgcc
    25201 ttcatcggtt tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt
    25261 ggtgattttc attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg
    25321 gtgcgttcta cggactgccc ggcgcgatag agatcgctgc ctaagcccgc ccctgcacaa
    25381 cctgcgtcta tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg
    25441 atgtccggtg gtaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac
    25501 ggaaatattt tcagcgcctg agcgcccgcg tcgagcgcgg taaaggcttc ggtcgccgtc
    25561 gcgcagccgg ggcaaacggt catgccgtag cccaccgcac ggcggatcac ttcactatgg
    25621 atattgggcg taacgatgag ctggcagctc atcctggcga gtgcatcgac ctgttcaggt
    25681 ttcagtaccg tacctgcgcc aatcaacgcc ttatcgccat acgcatcaac gatggcggga
    25741 atgcttttct cccattgagg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca
    25801 atcaccgcgc caacatgcgc cagcgcctcg tcgggcgtaa tgccgcgcaa aatggcgatc
    25861 agcgggagtt tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt
    25921 gtcgcccgcc accgccgtca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc
    25981 ggtcagcgat gttccggcga caagggtgat ggcgtgttga tgggtcacat agtcgcgcat
    26041 actggcaacc tctgcgccaa tcaacaaacc agagagaaat tcgctgacct gttcgcgggg
    26101 aagtgttccc agcacatgcg aggcgcgaac ttcaaaaagc tgcggcaata tggcgggagc
    26161 attaaagcca cgctcaagac cagccgcgaa ggcatcggca gagttttcct gcggcggcaa
    26221 acctgcgcca atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt
    26281 gcgaaaatcg ttgatttgct ggctatcggc ctgcacccat ttgcaatggg taccgggcat
    26341 aacataaaga gaggaaggag cgagagcacg cgcgccgatc aattgtgttt cttcgccgcg
    26401 catcacattg tggttatcgt catgagagac gcataatccg ggaataatcc agatattgtc
    26461 gccaaccgac gttaattgat cgccaataga cgaaaaacgg gcaggaacag ataaatacgg
    26521 tgcaactttc cagccgacat tgctgccgac cattcctgcc atcaccactg gcgtattctc
    26581 ttcacgccag tcggtcgtga cttctgctaa caccgcagcc ggagattttc cgttcaggcg
    26641 cgtgacgcct gcttctgatt gcctgctgtc caggcagtgg tcgccctgat aaagccaggc
    26701 gcgcagattg gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt
    26761 aaccttcgtg tcgagctggc gatcatggta agcgccgcct gctctgccgc atcgccgtcc
    26821 tgatgccgta ttgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc
    26881 tcatcgccca tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta
    26941 agttgctgta acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc
    27001 gcttcgttaa acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca
    27061 atctgcgcca gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat agccggctct
    27121 accaggtttc gcacttcgct cattgcactg ataagccgtg ggtcatagtc gttttccagc
    27181 acccattgca gtacgtcagt gtcgaggtaa ttccactggt tacgcggagc cacaaacgcc
    27241 ccgcgataac gtttcatttc aatcagccgc ttcgccatca gcgaacgaaa cacctcacgg
    27301 atgatgttgc gcgaggttgc aaactcttca cagagctccg cctcagccgg aagtggagag
    27361 cctggtacgt atttgccgtg aacgatctgt ttacccagcg taataacgat gcgatcggtt
    27421 ttattgagag tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc
    27481 gatgcataac gcggcaactt tgtagtacca gcgtgatgac gttcgcgttt gctgtgcgcc
    27541 taatgtagta caaacttata ttgttgtact acaatttaga tcacaaaaag aacaatacat
    27601 aaaaaatgac atgcgtcggg cagaaatctg aaaagggatg tcaggcgcta aacaggaggg
    27661 aaagaagagt atgctttcaa cggcttagct actcgtttaa aggattaatc atgaagttga
    27721 attttaaggg attttttaag gctgccggtt tgtgttcttt ggcatttgcg ttgaccggat
    27781 gtatcacctg gggattggtt tccaatactg caagccgtcc acatgccgag caatggcggt
    27841 cagacacgat taagggttta tcgctggctg aagacagtaa cggtacgaaa ggctatgttt
    27901 ttgttggtga atcgttggac tatctgttga ccaccggcgg cgatgaggtg gttaaaatgc
    27961 tgaacgatcc ggcaatccat ggtgaaagaa tcaccgtttc tgataacgcg aaatttattt
    28021 taagcagcag taataagaat ttctctggtg ctattacgct ctattacgac tggaacaacg
    28081 aagaagataa ggcactggca acacaatatg gttttatttg tgatacccgg cgttgtacat
    28141 ggatgctgga cggtttgaaa ggctcaatcc accagaaaaa taaaaaagcg gattactcaa
    28201 acgtgatggt tttccatcaa ccctttacgg tgggatttta tgagtacaaa gcaacagatg
    28261 gcgtcccgcg cggcctcgtt aatgcgctgc tgccagtcac tcttacgctg gatatagtaa
    28321 cgtcaccact ccagttcctg attttatgca cgaccagaaa ttgttaatcg ctgcgggcaa
    28381 acgcattcat gccgtttgcc cattcactaa ttaattcagc acatacttct caatagcaaa
    28441 tgccacgcca tcttcaaggt tggatttggt gacaaagttc gccacttctt tcaccgaagg
    28501 aatagcgtta tccatcgcca caccgacgcc tgcatattca atcattgcga tgtcgttttc
    28561 ctgatcgcca atcgccatga tttcttccgg tttaatacct aacacgtcgg ccagtgattt
    28621 cacacccgta cctttgttaa cgcgtttatc gaggatttcg aggaagtacg gcgcactttt
    28681 cagcacggtg tatttttctt tcacttcctg cggaatacgc gcgatggcct ggtcgaggat
    28741 ggcaggttca tcaatcatca tcactttcag gaactgggta ttggggtcca ttttctccgc
    28801 ttcgcagaac accagcggaa tggtggcaac gaaagattca tgcaccgtgt agtagctgat
    28861 atcacgattg gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc
    28921 gcgagagagt ttttccagga aacggtagtc gtcatagctg agagtggttt gcgccacggt
    28981 gctaccatca gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg
    29041 ctgttccata tgcagctctt tcaggtaatt gtgcacacct gcataagggc gacccgtcgt
    29101 tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga
    29161 aatggtgtga tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc
    29221 catgagttcc ccaggtagat tggttcctga cccatgctaa cgcgattccg ctcaaaaatc
    29281 agtacaacac ccgagggaaa agggggatgc aacgcgcgtt gcgtgctccc cttttgctta
    29341 gcggaagagt ttccctttca gcagttccat gcctgcggaa agcagatcgt tattggcttg
    29401 tggtgacact tcaccttgcg gtgagagtgc atcaataatc ttcggcaatt gttctgccag
    29461 taaactggaa gctgtactgg tatccacgcc cagtttttgc ccgagatcgg acaccgcatt
    29521 tgtgccgagc gccgattcca gttgctcgcc gctaactggt tggttgccct gttgattact
    29581 cagccaggtt gagagaatgg cacctaaacc gccactttgc agtttttcca gcagcacctg
    29641 aatgccgccc tgctcctcaa cccaacttaa aatcgcctga tatttccccg catcgccttt
    29701 cagaaaggcg ccgacaacgt catcaaaaag gcccatgata atcacctgta aagcgttacg
    29761 tgttgactca aaaagtatag ttctgtggga tgataattgc ggattgcagg aataaaaagg
    29821 gcggagatga tctccgccct tttcttatag cttcttgccg gatgcggcgt gaacgcctta
    29881 tccggcctac aaaaacatgc aaattcaata tattgcagga gtctcgtagg cctgataagc
    29941 gtagcgcatc aggcacgctc gcattgttaa cgccattaaa tatcaatatt cgccgctttc
    30001 agagcgttct cttcaataaa cgcacggcgc ggttcaacgg cgtcgcccat aagcgtagtg
    30061 aacaactggt cggcagcaat cgcatcttta acggtaacgc gcagcatacg acggctttcc
    30121 gggtccatgg tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc
    30181 tggatggaga ggccgcgacg ggactctttc accagccagt ccagcgcctg ctcgaagctg
    30241 gctaccggct gacgacgttc gccacgttcg ataaatgcat cttcttccag caagccacgc
    30301 agtttctcac ccagcgtgca gatacgacga tattcgccgc cagtgataaa ttcgtgatcc
    30361 agcggatagt cagtatccac accgtgggta cgcacgcgaa caatcggctc gaacaggttt
    30421 tgttcggcat tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc
    30481 agttcgctga ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct
    30541 tccgtcagcg tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc
    30601 atgcggttga tcattttctg cgtcgcgttg tactcagaca ccagtttctc taacgcttcg
    30661 ccagccaatg ccggtgcact ggcgttggtg tgcagcgttg cgccatccag cgcgatggag
    30721 atctggtact gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc
    30781 actttgtaca gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc
    30841 tgacgataga agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg
    30901 gtcatgatga tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata
    30961 ccacaaccaa gcgcggtgat aagcgtcgcc acttcctggg aagagagcat cttatcgaag
    31021 cgcgctttct cgacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg
    31081 ttacgcccct gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcagag
    31141 agcgccggat cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc
    31201 gcacctttac ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca
    31261 tcgataattt tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc
    31321 agcagttcgt tcatctgctg ctcaaccgcc gatttcacct cagaagaaac cagtttgtct
    31381 ttggtctggg aggagaattt cgggtccggc actttcacgg aaacgaccgc aatcaggcct
    31441 tcacgcgcat cgtcaccggt ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc
    31501 atgtaggcgt tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg
    31561 tcacgctgcg gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac
    31621 tgcaacgcca cttcgacgcc aataccgtct ttctcggtgg agaagtagaa gatattcggg
    31681 tggatcggcg ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag
    31741 tggaagtggt cttctttgcc gtcgcgcttg tcgcgcagac gaatggaaac gccggagttg
    31801 aggaacgaca actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg
    31861 gtgaaggttt cgaggcttgg ccagaaacgc accatggtgc cggttttttc agtctcgccg
    31921 gtaaccgcca gcggggcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc
    31981 tcgcgctgga taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg
    32041 ccgtgcagac cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga
    32101 acggtcatga tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg
    32161 ccgcgcccgt catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct
    32221 ttacagtgac ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg
    32281 tgcagaccgg tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca
    32341 tccagccctt tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct
    32401 cgctcattta tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga
    32461 attttcgtcc gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga
    32521 ttgcgtcgct tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc
    32581 aaaatcatct atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg
    32641 cgccagacgt aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac
    32701 cggcgcaccg tcggcgcgaa tgcgtaagtc cgctttatgc gggccgtgcg cggtgtaggt
    32761 tagctggcga tcgcgttcaa aattacgttc cagcacctca gcgtactctg tctctttctc
    32821 ccagccgcgc tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaggtatc
    32881 ggccatatca gccgcgatac cggcgctata ctccgcgcgc caggtgctta tttgctccgc
    32941 cagcggaatc agttctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag
    33001 cgccgcattg cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg
    33061 ttcgttgtga aagcatcccc agtcgaggaa tgctcttctg tatttggggc cgccgttgag
    33121 taaagtaaac ccttctggcg ttatcaactg cattggcatc aggtgcgcca gttccgcgac
    33181 cttatgcccg tctgtaccgt cgatgcggac tttgctgtcg ccctgtttgt ctttggttaa
    33241 gccaatcgcc gtctcgcgct cttcgccctg taatcgcccg tggagaacaa acgcctcctg
    33301 ctcgtggcga atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt
    33361 atagatggct tccagcacgc tggttttgcc actaccgttg gcacctacca gaaagttaaa
    33421 gccgggagat aaagcgagat ccgcggtttc gatattgcgg aaatcgcgga tcaacaagcg
    33481 ggtgagggac attacagtct cattggcatg acaacatagg cagccgactg tgatgcggca
    33541 tcttcaatct gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc
    33601 agtgcgttca gaacatccag cacatagctg acgttgaagc cgatttccat ctccgcaccg
    33661 ctataggtaa cgtcgaggat ctcttccgct tcttcctgtt ctgggttgtt ggcggtgatt
    33721 ttcagctggt tttcgctgac atacagacgc acgccgcgga atttctcgtt agagagaatt
    33781 gccgcacggg caaacgcctg cttgagcaga tcacagccag cttccagatg cttgtccggg
    33841 ttcttcggca gaacgcgacg gtaatccggg aagcgaccat ccaccagttt cgaggtgaag
    33901 ataaagtcgc caacgtgggc gcgaatgttg ttgctgccaa tctgcacgcg cagcggattg
    33961 tcgccgccgt cgagcatacg catcagttca atcacgcctt tacgcggcac gatcaccgaa
    34021 tggcttggca aagattgacc aattggcatt gaacagactg ccagacggtg gccgtcggtc
    34081 gccacggtgc gcagttcttc accttcggtt tcaaacagca taccgtttaa gtaatagcga
    34141 acgtcctgat gcgccataga aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc
    34201 ggcagggtaa attcgacttc gctctgccag tcatcgaggt tcgggaaatc tgccgctggc
    34261 agggtagaca gcgaaaaacg gctacgcccg gagcgcacca gcatccgctc accttccagc
    34321 tgcacggcaa tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc
    34381 ggaacggtcg tcgcccctgg ttcgtgcggc tgaaccagcg caacgcgtgc caccatttcc
    34441 atctcgagat cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta
    34501 ccgagaatcg gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt
    34561 aataaatgct cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa
    34621 attagaaaaa tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttccggca
    34681 ggcatgaagc accgtcgtgt ggtcacgacc accaaaggca tcgccaatct ccggaagact
    34741 gtggttagtc agctctttcg ccagcgccat cgccatttgg cgcggacggg ccaccgagcg
    34801 ggatcgacgc ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt
    34861 ctgaatattg tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc
    34921 acgcacgaag tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt
    34981 cagcgcgcct tccaactcac gtacgttaga tcgtagacgc ttggcgataa aaaacgccac
    35041 ttcgcccggc aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg ccacgcgggt
    35101 ttccagctct ggcggttcga tcgccacagt cagtccccag ccgaagcggg atttcaaacg
    35161 atcctcaacg ccgttgatct ctttcggata gcgatcggag gtgagaatga tctgttgatt
    35221 accttccagc agggcgttga aggtgtggaa aaactcttcc tgagatcgtt ctttattagc
    35281 aaaaaactga atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc
    35341 ttcgatcgcg ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat
    35401 ataaaccact ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagcag
    35461 gtgagtttta cccaaacccg tgccgccata aaggaacaac gggttataag caccgccagg
    35521 gttatctgcc acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa
    35581 gttatcaaac gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggagccgg
    35641 gacgttatcc caacccgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac
    35701 ctgtgcaggg gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt
    35761 ggtgccgact tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc
    35821 attgatatta ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta
    35881 cagggccagc gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc
    35941 tgtggctggt aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac
    36001 ggcggactcc actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg
    36061 acgtacgtcg aaaagaccct gcatgagggt gacgcacgaa ccgctgtctg cggttatatg
    36121 cccgatcaag atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag
    36181 atcttctgtt tctcacagat tttcccgatt tatccacagg actttccaga actcgctaag
    36241 tgtaaacgat cctgccgcgg gccgggcacg atttacgccg catattggaa aatttaatga
    36301 ccatagacaa aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta
    36361 cccatcagcc cgtataatcc tccacccggc gcgccatgct ggttttcact ggtgtgaggc
    36421 cgtacatttt ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac
    36481 tccggagtgt acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat
    36541 cgtttcattt ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa
    36601 atcgccatga aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc
    36661 cgtgctcgta tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc
    36721 cgcgctcgtc tgaccgtttc taagtaataa agctaacccc tgagtggtta agctcgcatt
    36781 tcccagggag ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca
    36841 acgggctggc acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg
    36901 tatcggtctt acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa
    36961 acgtctgacg cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt
    37021 ggtggtggcg aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga
    37081 aaaattatgg cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc
    37141 tatcaacgcc tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca
    37201 agctacggaa ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg
    37261 aaacgcgtat taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga
    37321 ccatttgata ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt
    37381 tgctgttcgt gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc
    37441 aggcccaaca gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg
    37501 taccggccag tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca
    37561 tcaacacccg tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga
    37621 actctaccca gccgttccag ctgctggaaa cttcaccgca gtttatttat caggcacaga
    37681 gcggtctgac cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata
    37741 acgttgaaaa ggacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga
    37801 cgtataccga cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt
    37861 acgctgtcaa cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcct
    37921 cgtttggtca gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca
    37981 acttcgcact gcacaccttc cgtggcgcgg cgtactccac gcctgacgag aagtacgaga
    38041 aatacaagtt cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt
    38101 gggtggcgat gctgcaacag tatttcgcga cggcatggat cccgcataac gacggtacca
    38161 acaacttcta taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc
    38221 cggtactggt tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg
    38281 aaatccagga caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt
    38341 ggttgtggtt catttctcag ccgctgttca aactgctgaa atggatccat agctttgtgg
    38401 gtaactgggg cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc
    38461 tgaccaaagc gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg
    38521 caatgcgtga gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt
    38581 acaaagctga gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa
    38641 tcttcctggc gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg
    38701 cactgtggat ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg
    38761 gcgtaacgat gttcttcatt cagaagatgt cgccgaccac tgtgaccgac ccgatgcagc
    38821 agaagatcat gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag
    38881 gtctggtgct gtactatatc gtcagcaacc tggtaaccat tatccagcag cagctgattt
    38941 accgtggtct ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg
    39001 agttttcgct aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggcggata
    39061 agcaccgcac atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg
    39121 tagcccaggc cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcttca
    39181 aagcccgtga agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt
    39241 atcttccgtt taaagacgcc gacggcagcg tgctcgatca ggggattgcg ctatggttcc
    39301 ctggcccgaa ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg
    39361 tgatcctcga cctgctgtta aaacgcattc tgaccattcc cggcctgcgg attgctcgcc
    39421 cgggtgagtt ttccgaacgc gcgtttctta acgataaact cgacttagcc caggccgagg
    39481 cgattgcgga tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc
    39541 tgcaaggcgc attctccgca cgggttaatc atctggtaga agccctcacc cacttgcgca
    39601 tttacgttga agcggcaatt gatttccccg atgaagagat cgatttcctc tccgacggca
    39661 aaattgaagc ccagcttaat gacgtgattg ccgatctcga tgcagtgcgt gctgaagcac
    39721 gtcagggtag tttgttgcgc gaagggatga aagtggtgat tgccggacgt cctaacgccg
    39781 gtaaatcgag cctgttaaac gcgctggctg gccgtgaagc agcaatcgta accgatatcg
    39841 ccggaactac gcgtgacgtg ttgcgtgagc atatccacat tgacggaatg ccgctgcata
    39901 tcatcgacac cgccgggcta cgtgaagcca gcgatgaagt ggaacgtatt ggtatcgagc
    39961 gcgcgtggca agaaattgaa caagccgacc gcgtgctgtt tatggtcgat ggcaccacga
    40021 cagacgccgt tgatccagca gagatctggc cggagtttat cgcccgtctg ccagcgaaac
    40081 tgccgatcac cgtggtgcgc aataaagccg atatcaccgg agaaacgttg gggatgagcg
    40141 aagtgaacgg tcacgcgtta attcgtctct ctgcaaggac tggtgaaggt gtggacgtgc
    40201 tgcgtaacca tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg
    40261 cgcgtcgtcg tcacctacag gcactggaac aggcggcaga gcacctgcaa cagggtaaag
    40321 cgcaactgtt aggagcctgg gcaggcgagc tgctggcgga agaactgcgt ctggcgcagc
    40381 agaacttaag cgagatcacc ggggaattta cttctgacga cctgctgggg cgaattttct
    40441 ccagcttctg tattggtaag taaccgcgct tgcgaagccg cattctgact gtcagatgcg
    40501 gcttcgcttc attgttagca ctcctgttac tcctcaaccc tttttttaaa cattaaaatt
    40561 cttacgtaat ttataatctt taaaaaaagc
//
LOCUS       DAZGAP010000013        39977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGAP010000013 DAZGAP010000000
VERSION     DAZGAP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.013"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..1225)
                     /locus_tag="ACHLAM_000639"
     CDS             complement(101..1225)
                     /locus_tag="ACHLAM_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO7852221.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            1358..2911
                     /locus_tag="ACHLAM_000640"
     CDS             1358..2911
                     /locus_tag="ACHLAM_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO7852222.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3293..4183
                     /gene="metF"
                     /locus_tag="ACHLAM_000641"
     CDS             3293..4183
                     /gene="metF"
                     /locus_tag="ACHLAM_000641"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO7852223.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4512..6692
                     /gene="katG"
                     /locus_tag="ACHLAM_000642"
     CDS             4512..6692
                     /gene="katG"
                     /locus_tag="ACHLAM_000642"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO7852224.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6786..7691
                     /gene="yijE"
                     /locus_tag="ACHLAM_000643"
     CDS             6786..7691
                     /gene="yijE"
                     /locus_tag="ACHLAM_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO7852225.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7718..8335)
                     /gene="yijF"
                     /locus_tag="ACHLAM_000644"
     CDS             complement(7718..8335)
                     /gene="yijF"
                     /locus_tag="ACHLAM_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO7852226.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(8611..9714)
                     /gene="gldA"
                     /locus_tag="ACHLAM_000645"
     CDS             complement(8611..9714)
                     /gene="gldA"
                     /locus_tag="ACHLAM_000645"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO7852227.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9725..10387)
                     /gene="fsa"
                     /locus_tag="ACHLAM_000646"
     CDS             complement(9725..10387)
                     /gene="fsa"
                     /locus_tag="ACHLAM_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7852228.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(10399..12900)
                     /gene="ptsP"
                     /locus_tag="ACHLAM_000647"
     CDS             complement(10399..12900)
                     /gene="ptsP"
                     /locus_tag="ACHLAM_000647"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7852229.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13209..14288
                     /gene="frwC"
                     /locus_tag="ACHLAM_000648"
     CDS             13209..14288
                     /gene="frwC"
                     /locus_tag="ACHLAM_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO7852230.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            14303..14623
                     /gene="frwB"
                     /locus_tag="ACHLAM_000649"
     CDS             14303..14623
                     /gene="frwB"
                     /locus_tag="ACHLAM_000649"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7852231.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14674..16971
                     /gene="pflD"
                     /locus_tag="ACHLAM_000650"
     CDS             14674..16971
                     /gene="pflD"
                     /locus_tag="ACHLAM_000650"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7852232.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGS
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRSGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16937..17815
                     /gene="pflC"
                     /locus_tag="ACHLAM_000651"
     CDS             16937..17815
                     /gene="pflC"
                     /locus_tag="ACHLAM_000651"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO7852233.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSQENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17817..18158
                     /gene="frwD"
                     /locus_tag="ACHLAM_000652"
     CDS             17817..18158
                     /gene="frwD"
                     /locus_tag="ACHLAM_000652"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7852234.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18145..18996)
                     /gene="yijO"
                     /locus_tag="ACHLAM_000653"
     CDS             complement(18145..18996)
                     /gene="yijO"
                     /locus_tag="ACHLAM_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7852235.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGATGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19222..20955)
                     /gene="eptC"
                     /locus_tag="ACHLAM_000654"
     CDS             complement(19222..20955)
                     /gene="eptC"
                     /locus_tag="ACHLAM_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO7852236.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21138..23789)
                     /gene="ppc"
                     /locus_tag="ACHLAM_000655"
     CDS             complement(21138..23789)
                     /gene="ppc"
                     /locus_tag="ACHLAM_000655"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO7852237.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24091..25242)
                     /gene="argE"
                     /locus_tag="ACHLAM_000656"
     CDS             complement(24091..25242)
                     /gene="argE"
                     /locus_tag="ACHLAM_000656"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO7852238.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25396..26400
                     /gene="argC"
                     /locus_tag="ACHLAM_000657"
     CDS             25396..26400
                     /gene="argC"
                     /locus_tag="ACHLAM_000657"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO7852239.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26408..27184
                     /gene="argB"
                     /locus_tag="ACHLAM_000658"
     CDS             26408..27184
                     /gene="argB"
                     /locus_tag="ACHLAM_000658"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO7852240.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27245..28618
                     /gene="argH"
                     /locus_tag="ACHLAM_000659"
     CDS             27245..28618
                     /gene="argH"
                     /locus_tag="ACHLAM_000659"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO7852241.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVESKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            29116..30330
                     /gene="manD"
                     /locus_tag="ACHLAM_000660"
     CDS             29116..30330
                     /gene="manD"
                     /locus_tag="ACHLAM_000660"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO7852242.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            30397..31680
                     /locus_tag="ACHLAM_000661"
     CDS             30397..31680
                     /locus_tag="ACHLAM_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7852243.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            31932..32849
                     /gene="oxyR"
                     /locus_tag="ACHLAM_000662"
     CDS             31932..32849
                     /gene="oxyR"
                     /locus_tag="ACHLAM_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO7852244.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32832..34232)
                     /gene="sthA"
                     /locus_tag="ACHLAM_000663"
     CDS             complement(32832..34232)
                     /gene="sthA"
                     /locus_tag="ACHLAM_000663"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO7852245.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34564..35214
                     /gene="fabR"
                     /locus_tag="ACHLAM_000664"
     CDS             34564..35214
                     /gene="fabR"
                     /locus_tag="ACHLAM_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO7852246.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35214..35573
                     /gene="yijD"
                     /locus_tag="ACHLAM_000665"
     CDS             35214..35573
                     /gene="yijD"
                     /locus_tag="ACHLAM_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO7852247.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35613..36713)
                     /gene="trmA"
                     /locus_tag="ACHLAM_000666"
     CDS             complement(35613..36713)
                     /gene="trmA"
                     /locus_tag="ACHLAM_000666"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO7852248.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36827..37017
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37082..38965
                     /gene="btuB"
                     /locus_tag="ACHLAM_000667"
     CDS             37082..38965
                     /gene="btuB"
                     /locus_tag="ACHLAM_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO7852249.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            38910..39767
                     /gene="murI"
                     /locus_tag="ACHLAM_000668"
     CDS             38910..39767
                     /gene="murI"
                     /locus_tag="ACHLAM_000668"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO7852250.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9305 a         9823 c        10836 g        10013 t
ORIGIN      
        1 cattgaccat gattatattt cacggtcata catttatata tttaattaaa tgaaaggtag
       61 tgttctctct taatttatgg tcgtttgtcg agtgtattga ctaacaagat ggagtgagcg
      121 attcattgta ttttaacgcc agtgaggtca gcatttgtgt tacgcgacct gcggataata
      181 acgcgctttg ggtaaagtcg tgttggtctg tcagccatgt gataaaacaa cgactggttg
      241 ccagagaaac atttgcgccc ctttctaagc aggctcccgt gagtaggcag accatcttgc
      301 agttattccc ctgctcccag cgctgccact gattttgcca ccaaccatgg tatgtcgttc
      361 ccttaaaatg ccagggcgta tcagcgttac tgtcactgat ttcttcttct acgaaccaaa
      421 gttcaccttg ttgttggcgg aactgatctg cccaggctgt aataattgcg cctcttagaa
      481 aacattcgtg tgaaataata tgcgttttga catctggata gcggattttc agtattctga
      541 aatgcccgtg tgccgtgata accgggcgta gtatggctcc cttaccgttg cgcttgagta
      601 cagcactgcc atactgaatt tcatcgaact ggctccggcg caagaattgc gcttcacgca
      661 tatctacctt ctgcacgata tcgcgatggt gcatagttgg ttctgtgttg cccttgtaac
      721 tgtaccgcag actgtctcct gtctgatgtt gcgtataatt cgtggagaga tgaagaatgt
      781 tgccacgagt ggcgtcaaga ctgactaaaa catacagttt ttgtactgcg ctggcccctt
      841 gaaagggtat gaacagttga acggtttcaa tggcctgggg atgcgtaatt tctttctgat
      901 actgtttagg cgtccagact tttttgcagt tacggcattg aatgcgttgc gaaccctgtg
      961 gattgtgacc ataacaaatc atgtctgtgc cataacatac cgggcagaaa tgacccttct
     1021 ccgaggccca cgtagataag tgaactgaca accagtcacg aaattgttgc tcgtcaaaca
     1081 acggcgggta actgccacat gcccgacagt gcagtgccgg ataacccagt ttatagtctg
     1141 gccaactgta atcttccgat gaggccaatc ccaaatttcg gcaaccgaac gttttacagg
     1201 cattaatcgt aaacagagtc gacatatatc accaacgatt tcacaaggtc gttgattttg
     1261 tccggcaact atcccctgca gatgtgatta ctctcacata aataagtctt tcagaacgta
     1321 atgtagcaat cctttatctc actgaaaggg attgtttatg aaaattaaaa tacttgctgc
     1381 gggtatcgca ttaacactgc cgttctgggc ttgcgctaaa gatgtcacta ttatttatac
     1441 caacgacctc catgcgcatg tagaacctta taaagtgccg tggatcgctg acggtaaacg
     1501 ggatattggc ggctgggcaa atatcactac gctggttaag caagaaaaag caaaaaataa
     1561 agcgacatgg ttttttgatg cgggcgacta ttttaccggg ccatatatca gcagcctgac
     1621 caaaggtaag gctattatcg atattatgaa tactatgccg tttgatgccg tcacgatagg
     1681 caaccatgaa tttgaccacg gctgggacaa cacattatta cagttgagtc aggcaaaatt
     1741 cccgattgtg cagggcaatg ttttttatca gaacagcagt aaatcattct gggataagcc
     1801 ctataccatc atcgaaaaag acggtgtgaa aataggcgtt ataggtttgc acggtgtatt
     1861 tgcctttaat gacacagtat ctgctgccac gcgagtgggt attgaggcgc gtgatgaaat
     1921 taaatggcta caacgttata tcgatgaact taaaggcaag gttgatttga ccgtcgccct
     1981 gatccatgaa ggtgttccgg cccgccagtc cagtatggga ggcacagatg tgcgtcgcgc
     2041 gctggataaa gatattcaga cggcaagtca ggtgaaaggg ctggatattt tgatcaccgg
     2101 acatgcacat gtgggtacgc cggaaccgat taaagtcggc aatacgctca tcctctccac
     2161 tgacagcggc gggattgatg tgggcaaact ggttctcgac tacaaagaga agccgcataa
     2221 ttttacggtg aaaaacttcg agcttaaaac catttacgct gacgagtgga agcccgatcc
     2281 acaaacgaaa caggtgatcg acggctggaa taaaaagctt gatgaagtgg tgcaacaaac
     2341 ggtggcgcaa tcgccggttg aattaaaacg tgcctatggt gaatcggctt ccctcgggaa
     2401 cctggcggct gacgctttac tggtagcggc gggtaaaaac acccaattgg cgttaaccaa
     2461 ctctggcggc attcgcaatg agatccccgc aggggcgatc acgatgggcg gcgtcatcag
     2521 tacgttcccg ttccccaacg aactggtgac gatggaactc acgggtaaac aattacgcag
     2581 tctgatggaa cacggcgcaa gtttaagcaa tggcgtttta caggtatcga aaggcctgga
     2641 aatgaagtac gacagcagca agccggttgg tcagcgggta atcacactga ctctgaatgg
     2701 caaacccatt gaagatgcga cggtttatca cattgctact cagagtttcc ttgctgatgg
     2761 tggagatggt tttaccgcct ttaccgaagg gaaagcgcgt aacacaacgg gcggttatta
     2821 cgtttatcac gcggtggttg attacttcaa agcgggcaac accattacgg atgaacagct
     2881 caacggtatg cgcgtgaagg atatcaaata aaagtgcgca ctgaattgac tcaactgttt
     2941 gttacccgtt gattgcgcag atcctgtatt cggcctacgc gagggcgcag gccgaatcag
     3001 atgccttagc atcaccatcc ggtggcagga cgttgttttt cgaattagcc caggtgtcgc
     3061 tgtgttgtca gaatatgagt caaaacgtgc gaggtggatt gtgctataga gaaatctttt
     3121 atcgcctcta attaagatga gtttttttca tcttccctga tttttcctca ccatcattgg
     3181 ttatttttcg gttgacgccc ttcggctttt ccttcatctt tacatctgga cgtctaaacg
     3241 gatagatgtg cacaacacaa catataacga taagcgattg atgaggtaag gtatgagctt
     3301 ttttcacgcc agccagcggg atgccctgaa tcagagtctg gcagaagtcc aggggcagat
     3361 taacgtttcg ttcgaatttt tcccgccgcg taccagtgaa atggagcaga ccctgtggaa
     3421 ctccatcgat cgccttagca gcctgaaacc gaagtttgta tcggtgacct atggcgcaaa
     3481 ctccggcgag cgcgaccgta cgcacagcat tattaaaggc attaaagatc gcaccggcct
     3541 ggaagcggca ccgcatctta cctgtattga tgcgacgcct gccgagctgc ggaccattgc
     3601 acgcgactac tggaataacg gtattcgcca tatcgtggcg ctgcgtggcg acctgccgcc
     3661 gggaagtggt aagccagaaa tgtatgcttc tgacctggtg acactgttaa aagaagtggc
     3721 agatttcgat atctccgtgg cggcgtatcc ggaagtacat ccagaagcga aaagtgccca
     3781 ggccgattta ctcaacctga gacgaaaagt ggatgccgga gccaaccgcg ccattactca
     3841 gttcttcttc gatgtcgaaa gctacctgcg ttttcgcgat cgctgcgtct cggcaggcat
     3901 tgatgtggaa atcattccgg gaattttgcc ggtttcgaac tttaaacagg cgaagaaatt
     3961 tgccgacatg accaacgtgc gtattccggc gtggatggca caaatgttcg acggcctgga
     4021 tgacgatgct gaaacccgca agctggtggg cgcgaatatt gccatggata tggtgaagat
     4081 tttaagccgt gaaggagtga aagatttcca cttctatacg cttaaccgcg ctgaaatgag
     4141 ttacgcgatt tgccatacac tgggtgttcg acctggttta taaatagtgt gccttttgtg
     4201 aaaattacac agtgatcaca aattttaaac agagcacaaa atgctgcctc gaaatgaggg
     4261 cgggaaaata aggttatcag ccttgttttc tccctcatta cttgaaggat acaaatctaa
     4321 aacccttttt tataaagcat ttgtcctaaa tcggacataa tcaaaaagtc ttaattacga
     4381 tcaatttgat ctacatctct ttaaccaaca atatgtaaga tctcaactat cgcatccgtg
     4441 gattaattca attataactt ctctctaacg ctgtgtatcg taacggtaac actgtagagg
     4501 ggagcacatt gatgagcacg tcagacgata tccataacac gacagccacc ggcaaatgcc
     4561 cgttccatca gggcggtcac gaccagagcg caggtggggg cacaaccacg cgcgactggt
     4621 ggccaaatca actccgtgtt gacctgttaa accaacattc taatcgttct aacccgctgg
     4681 gtgaggactt tgactaccgc aaagaattca gcaaattaga ttactacgga ctgaaaaaag
     4741 atctgaaagc cctgctgaca gaatctcaac cgtggtggcc tgccgactgg ggcagctacg
     4801 ccggtctgtt tattcgtatg gcctggcacg gtgcgggtac ttaccgttca atcgatgggc
     4861 gcggcggcgc gggtcgtggt cagcaacgtt ttgcaccgct gaactcctgg ccggataacg
     4921 taagcctcga taaagcgcgc cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa
     4981 tctcctgggc cgacctgttt atcctcgcgg gtaacgtggc gctggaaaac tccggcttcc
     5041 gtaccttcgg ttttggtgcc ggtcgtgaag acgtctggga accggatctg gatgttaact
     5101 ggggtgatga aaaagcctgg ctgacccacc gtcatccgga agcactggcg aaagcaccgc
     5161 tgggcgcaac cgagatgggc ctgatttacg ttaacccgga aggcccggat cacagcggcg
     5221 aaccgctttc tgcggcagca gctatccgcg cgaccttcgg caacatgggc atgaacgacg
     5281 aagaaaccgt ggcgctgatt gcgggtggtc atacgctggg taaaactcac ggtgccggtc
     5341 cgacatcaaa tgtaggtcct gatccagaag ctgcaccgat tgaagaacaa ggtttaggct
     5401 gggcgagcac ttacggcagc ggcgttggcg cagatgccat tacctctggt ctggaagtag
     5461 tctggaccca gacgccgacc caatggagca actatttctt cgagaacctg ttcaaatatg
     5521 agtgggtaca aactcgcagc ccggctggcg caatccagtt cgaagcggta gacgcaccgg
     5581 aaattatccc ggatccgttt gatccgtcga agaaacgtaa accgacgatg ctggtgaccg
     5641 acctgacgct gcgttttgat cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc
     5701 cgcaggcgtt caacgaagcc tttgctcgtg cttggttcaa actgacgcac agggatatgg
     5761 ggccgaaatc tcgttacatc gggccggaag taccgaaaga agatctgatc tggcaagatc
     5821 cgctgccgca gccgatctac aacccgaccg agcaggacat tatcgatctg aaattcgcga
     5881 ttgcggattc tggtctgtct gttagtgagc tggtatcggt ggcctgggcg tctgcttcta
     5941 ccttccgtgg tggcgacaaa cgtggtggtg ccaacggtgc gcgtctggca ttaatgccgc
     6001 agcgtgactg ggatgtgaac gccgcagccg ttcgtgctct gccagttctg gagaaaattc
     6061 agaaagagtc tggcaaagct tcgctggcgg atatcatcgt gctggcaggt gtggttggcg
     6121 ttgagaaagc cgcaagcgcc gcaggtttga gcattcatgt accgtttgcg ccgggccgcg
     6181 ttgatgcgcg tcaggatcag actgacattg agatgttcga actgctggag ccaattgctg
     6241 acggtttccg taactatcgc gctcgtctgg acgtttccac caccgagtca ctgttgattg
     6301 ataaagcaca gcaactgacg ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc
     6361 gcgtactggg tgccaacttc gatggcagca aaaacggcgt cttcactgac cgcgttggcg
     6421 tattgagcaa tgacttcttc gtgaacttgc tggatatgcg ttacgagtgg aaagcgaccg
     6481 acgaatcgaa agagctgttc gaaggccgtg accgcgaaac tggcgaagtg aaatacacag
     6541 ccagccgtgc ggatctggtc tttggttcta actctgtcct gcgtgcggtg gcggaagtct
     6601 acgccagcag cgatgcccac gagaagtttg ttaaagactt cgtggcagcg tgggtgaaag
     6661 tgatgaacct cgaccgtttc gacctgctgt aatctgacct tcttcagcga ctgcctttca
     6721 tgcagtcgct gaagtttctt taccggcgta tagtgtccac aggaaaacta cacactggat
     6781 ctctcatgtc tgccgcagga aagagcaacc cactggcaat cagtggcctg gttgtgctca
     6841 cacttatctg gagttatagc tggattttca tgaagcaagt caccagctac atcggtgcct
     6901 tcgactttac cgccttacgc tgcattttcg gcgctctcgt tttattcatc gtccttttat
     6961 tacgtggtcg cggaatgcgc ccgacaccgt ttaaatacac cttagccatt gccctgttac
     7021 aaacctgcgg gatggtcggc ctggcgcagt gggcgctggt cagcggagga gcggggaagg
     7081 tggcaatcct gagctatacc atgccgttct gggtggtaat tttcgccgcg ttgtttctcg
     7141 gtgaacgcct gcgacgcgga caatatttcg cgattctgat tgccgctttc ggcttatttt
     7201 tggtgttgca gccgtggcaa ctcgatttct cttcgatgaa aagcgccatg ctggcaatcc
     7261 tctccggtgt gagttggggg gcgagcgcga ttgttgctaa acgtctgtat gcccgccatc
     7321 cgcgcgtgga tttattgtcg ctaacatcct ggcagatgct gtatgcggcg ctggtgatga
     7381 gcgtggtcgc tttactggtg ccgcaacgtg aaattgactg gcaacccacc gtgttctggg
     7441 cgctggccta cagtgcgatt ctggcgacag cgctggcgtg gagcttatgg ttgtttgtat
     7501 tgaaaaactt gcctgccagt attgccagct taagcacact ggccgttccc gtttgcggcg
     7561 tactcttttc ctggtggctg ctcggtgaga atccgggggc agttgaaggt agcggtattg
     7621 tgctgattgt actggcactg gcgctggtga gccgtaagaa aaaagaagcc gtcagtgtaa
     7681 aaaggatctg aattttttct tcatgtgggg agatctctta tttagcaaaa taccgatagt
     7741 gccccaccat ccgccagcta aacagtacat cttcttcctg cgcgcctgcg ccaatgttat
     7801 gtatcaccag cggcgtaccg tcgcgggcga agccatctga caccacccca atatgtgcca
     7861 gcccgttatc taatcgccag gagacaatat cgcccgcttg atagtcactg gggttcttgc
     7921 tggtggggcg tgttttatcg tggcgggtaa accaggtttc cagattaggc acccggcggt
     7981 gatcgatgtt gctgtccggg cgctttaact gccatttttg cgggtacgca gcaaaattct
     8041 tcgccatatc ttcgtgcacc agtttctgca aatcgacctt ctggctgcgc aatgcacgga
     8101 tcaccacatc ggaacatacg ccgcgttctt gcgggacatc accgccagga taggtaagct
     8161 gcacatacgc cggatcgtaa aatagcgtgc tgccgatttg ctgtctggcg ccgtctgcga
     8221 tggcaagatt ggtgttggcc tggatttgta ccacggttgg tggaacggcg ggagatttta
     8281 aggagtaact ggtaaatacc gtcagaagac tgaataacgc cagtgaagct ttcatctgtt
     8341 tcgtccgtga aaataagagt cacagattcg accttccggg ctaacgattg ttctgtggag
     8401 aaaatgtcca gaagcggata aaaaaacacc cgaacaaggt ggaagtttca gattactggc
     8461 acagcgtgcc ggatgcggcg tgaacgcctt atccggccta caaaagtacg taaatttact
     8521 atattgcaga gattgcgtag gcctgataag cgtagcgcat caggcaattt tgcgttcaaa
     8581 ctcccggaca agccgggagt ttggagtagg ttattcccac tcttgcagga aacgctgacc
     8641 atactggtcg gctaccagca gtgcggcgta aacctgatct ggcgtcgcgc cgccaggcat
     8701 gttgtggatg gtttcacctt ctgcacatgc cgcttctgcc acaattcgca ttttcgccgg
     8761 gacatcttct ttaatatcca gttgagcgag agttattggc aaacctaccg catggctaag
     8821 tgcagctacg gtttcgattt cctccaccgg cgcgttttcc agaaccagct gcgtcagcgt
     8881 accaaatgcc actttttcac cgtggtaata gtgatgcgcg tccgggatag cggtcaggcc
     8941 gttatgcacc gcgtgagccg ccgccagccc gccgctttca aaaccaacgc cgctcaaata
     9001 ggtgttcgct tcaatcacgc gctccagcgc cggggttact acatgctgct cggcggcaag
     9061 catcgctttt tcgccttctt ccagcagggt gttgtagcac agttcagcca gtgccagcgc
     9121 cgcctgggtg cacttgccgc ccgccatggt ggtcgcgccg ctacgagagc aagcacgcgc
     9181 ttcaaaccag gttgccagcg catcgccgat acccgccgct aacagacgtg caggtgcgcc
     9241 agcgacgatt ttggtgtcga caatgaccat attcgggtta tttggcaaca tcagatagcg
     9301 gtcaaattca ccatcatcgg tgtagataac agacaatgcg ctgcacggtg cgtcggtaga
     9361 ggcgatagtc ggtgcgatcg ctaccggaac acccatgaaa tgtgccagtg ctttggcagt
     9421 atcgagagtt tttccgccac cgataccgag aattgcgcca cactgcgcag tctccgcgat
     9481 gccacgcaga cggtcgatct cattttgcga acattcaccg ccaaacggcg caatttctac
     9541 taccagtcca gcatctttaa agcttttctc gacagtggat tgagcaaaac ctaaaacaaa
     9601 tttgtcaccc accactaacc agcgttctgc cagcggcttc aggtattcgc ccagacgatt
     9661 aatcacatca gcgccctgga tgtatttacc cggtgattga ataatgcggt ccataattgc
     9721 tcctttagag atgagtagtg ccaaatgcgg cattccagtc gtgttcgaac ttctctatag
     9781 ctgactctac cgcaggggtg ttgagcattt gttgcgctac atctaagggc agggtgatgg
     9841 attcacatcc tgccagtaaa cagtccagcg cctgacgtgg tgttttaaag ctggcagcca
     9901 gcaccatgct ttctggcgca tgcatttcca acagcgcttg cagctcctgt acagtacgaa
     9961 tgccgtcacc gccctgggca tctacgcggt taacgtatgg agcaacgtat ttggctccag
    10021 ccagcgccgc cagtaatcct tgcgctgcgc tgtacactgc ggttcccagc gtagtaatgc
    10081 cttctttctt cagcatttta attgctgcca gaccttcaga ggttaccgga attttaacca
    10141 caatgcccgg aatagcgtcg cgcaggcgtt tcgcttcttt caccataccc tgcgcgtcgc
    10201 ggctcatggt ctgagcaaac agaattccct catcaccgat cgctttttga aggcgcggca
    10261 gcacttccca gatggactcc ttgctggcag cgataatgct cgggttagtt gtcaccccgg
    10321 caatcgggaa tatgcgtgcc agacgttcga cttctgcgac gttagcggtg tccagataca
    10381 gttccatgat gtttcctttt acagttccag ttcatgttgc agcaggctgg cgatagcgtc
    10441 ggcagaggcg gcgttaacca gcgcgttacg gaattcttcg tgcatgatgc gacgagcgag
    10501 gcgcgagaaa atgcgcatgt gctgatcgcc cgcagcatgt ttgttcaggg ttaacataat
    10561 gatgaattgc gcttcatcat cgccccagcg caccggcgct gccagacgcg ccacgctgat
    10621 ggtggattgc tcaatgtgtt ctgatttgct gtggggaatg gcaaaactaa agcccagacc
    10681 ggtagagaaa acggcctcgc gcgcccacaa gtcggcttcc agtttacgcg gatagcgaca
    10741 gcggcccgcc agcagcaggt tatcggtcat gcctttgagc acttcttctt tgctgcgcca
    10801 gtcgctttcc agtgtgatgc actcggcggt gaccagcggt gcgtcttgtt gggtcatgcg
    10861 gaattgcgcc agcaggtgtt ccacttccag cgaagtacgg caggccattg cctggttgag
    10921 caacttgcgg cactcacggc tatcaagttg cgccatccgc gctttcgccg ccggaattga
    10981 tggtgcgctc atgctgagtt catccagccc taaaccgacc agcaacggca gcacggaacc
    11041 tttcgctccc agttcaccgc acagaccaat ccatttgccc tggcgatgca ccgcctgcac
    11101 ggcgtaatcg agcgcccgca agaatgctgg attcaggctg ttgtagtgac gagtcacctt
    11161 agcgttatcg cgatccactg ccagtagata ttgcgtcagg tcattactac caatactaaa
    11221 gaaatcaatc tcttcgcagc attgatcgat gatgaacatc accgacggca cttccagcat
    11281 gatgccgagt ggaatcttct catcaaacgg aatgtgttcg ttacgcagtt gctgtttggc
    11341 ttccgccagt ttttctttca cccataagat ctcttccatt gaggagatca tcgggatcat
    11401 gattttcagg ctgccgtgag cagaggcgcg caggatcgac cgtagttgtg tggtaaacaa
    11461 cgacgcgtat tcttcataaa tacgcaccgc gcgataaccg aggaacgggt ttgcctctgc
    11521 gggaatattc agataatcaa cgggtttgtc accgccaata tccatagtgc gcacaataat
    11581 gctgcgtccg ttggcggact ccagcgcctg acaaaaaatg ttgtacagct cgctttcgcc
    11641 cggtgcactg gtgcgatcca tatagagcat ttcagtgcgg aataaaccaa cgccttccgc
    11701 accattgccg aatgcggcct gcgcttccac ggagtgagcg atgttagcgg caatttcaat
    11761 gcggataccg tcagcggtac gggcttgttg ggtcagccag acacgctgtt gctcacgcag
    11821 ggcgtcctgt acgcgggctt cttgctgata ataacgggcc acggcttccc ctggctcaac
    11881 cacaatcgcc cctgcgttgc cgtcgatata aatcgtttga tgctgccacg gagtaagggc
    11941 atcaatatcc acacctacca gcgtcggaat gttgaacgaa cgggcaagga tcaccgtatg
    12001 tgaggtggtg ccgccacttt tcagtaatag acctttgagg tgatttttat ccagttcgag
    12061 gaactggctg ggggtcagtt catcagccat acaaatggcg ggctgcgtca gtttgcccgg
    12121 tgccgggaag cgttgctcac cgtagatttg ctggagtaac tggaagcaga cgtcgcgtac
    12181 gtccagggca cgttcttgca ggtagctgct gctggaacgg gcgaactctt cgcaaaagtg
    12241 attcgcactg gcaacaatcg cttcggcgca gcttaatccg gcgctgacac ctgccagtaa
    12301 atgttcgcgc agggaagtat cgccagccag ggatcggtga gcttccagta tcgcgctggt
    12361 agcaccgtcg ctatccagca gacgaaattc aatgtttttc atcaccagtg tcaggccgtt
    12421 ttccagcgcg gattgctcgg cgtcaacgcc tttggcagca ggaagattac ccagcgcatt
    12481 gagatctaaa gaagagatcg gcgtcagaat gccgcctgca ctaccactgc acacggtgcg
    12541 agcacggata atttgcggat tcagattggt cagtgaaacc ggcagtggtt ccagttcgtc
    12601 agattttact tccgccagcg gtgcgtcgca gtgggggaat tcatcgcgca gccattggct
    12661 taaacgctgg tgcgcttcct gttcgtcggc cccggaaatc agtaactggc agttatcgcc
    12721 caccagcgta tcggtgccaa tcagcgcaag ggcgcttttg gcgttgccct tgcggtcagt
    12781 gcgcaggtta tgccactcaa tttgtgatga aaaagtatta cacagcgttt caacgtggct
    12841 tgccggacgt gcatgtacgc cgttaggtag ctcacaaata aattccacaa tcagggccat
    12901 tgcctctctc ccggtacggt tttctgttta caggataaga gcgcacggca acggcctgcc
    12961 atgtgacaaa tctgccaaaa gctggacaaa tgtaatgtaa ccgtcaattt gcgacgcgtc
    13021 tcacaagacg ccgttttgcg gcatgcttcc ggatcatcgc aagttatgag gcggatcgca
    13081 tttttgtact gatattacaa aagtccagta aatggccttt ttatccactg tttgctccgc
    13141 ctgcgattgc ctattgtttc ctccatcaaa aatatggaca tgggcttttg caagcccggg
    13201 agcagcttat gaatgagttg gtgcagatcc tgaaaaatac ccgtcagcat ttaatgacgg
    13261 gcgtttcaca catgattccc ttcgtggtat cgggcggtat tttgctggcg gtttccgtca
    13321 tgttgtatgg caaaggcgca gtgccggatg ccgtagccga tccgaatctg aaaaaactgt
    13381 ttgatatcgg cgttgcgggt ttgacgctga tggtgccttt cctcgccgct tacattggtt
    13441 actccattgc agagcgttct gcgctggctc cgtgcgctat cggtgcctgg gttggtaaca
    13501 gctttggtgc gggcttcttt ggtgcactga tcgccgggat tatcggcggc atcgtggtgc
    13561 attacctgaa gaaaattccg gtgcataaag ttctgcgctc ggtgatgcca atcttcatca
    13621 ttccgatcgt cggcacactg attaccgcag gcatcatgat gtgggggctg ggcgagcctg
    13681 tgggggcatt gaccaacagc ctgactcagt ggcttcaggg gatgcagcag ggcagcattg
    13741 ttatgctggc ggtgatcatg ggcctgatgc tggcgttcga tatgggcggt ccggttaaca
    13801 aagtggccta tgccttcatg ctgatttgcg ttgctcaggg tgtttatacc gtggtggcca
    13861 ttgctgccgt gggtatttgt attccgccgc tggggatggg gctggcgacg ctgattggtc
    13921 gtaaaaattt ctccgcagaa gagcgcgaaa ccggtaaagc ggcgctggtg atggggtgcg
    13981 ttggggttac tgaaggggcg attcctttcg ccgctgccga tccgctgcgt gttattcctt
    14041 ccatcatggt cggttcagtt tgtggtgcag taactgcggc gctggtcggt gcgcagtgct
    14101 atgcaggctg gggcggtctg attgtgctgc cggtggttga aggcaagctg ggttatatcg
    14161 cagcagtagc tgtcggagca gtggtgacgg ctgtttgtgt gaacgtgctg aaaagtctgg
    14221 cgcgtaaaaa tgggtcttcg actgatgaaa aagaagacga cctggatttg gattttgaaa
    14281 ttaattaatt gaggaacgag ccatgacgaa aattattgca gtaaccgcgt gtccatctgg
    14341 tgttgcccat acctatatgg cggcagaggc gctggaaagt gccgcgaaag cgaaaggctg
    14401 ggaagtgaaa gtagaaaccc agggctcgat tggtctggaa aacgaactga ctgcggaaga
    14461 cgtggcgagc gccgatatgg tgatcctgac caaagatatc ggcatcaagt ttgaagagcg
    14521 ttttgctggc aagaccatcg tgcgcgtcaa catcagcgac gcggtaaaac gcgctgatgc
    14581 cattatgagc aaaattgaag ctcacctggc acaaaccgct taagttctta tcccgcccgt
    14641 aagggcgggt tcgctttccc acaggagttc ctcatgacga atcgtatctc tcgcctcaaa
    14701 actgcactgt ttgccaatac ccgtgaaatc tcgctggagc gtgcgctgct ttataccgcc
    14761 agccatcggc aaaccgaagg cgaaccggtg atattgcgcc gggcgaaagc aacagcgtat
    14821 atccttgaac atgttgaaat ttcgattcgt gatgaagaac tgattgccgg taaccgcacc
    14881 gtaaaaccgc gcgccgggat tatgtcgccg gaaatggacc cttactggct gctgaaagag
    14941 ctggatcaat tcccgacgcg tccgcaggac cgctttgcta tcagcgaaga agataaacgt
    15001 atctaccgtg aagagttgtt cccgtactgg gaaaaacgtt cgatgaaaga tttcattaac
    15061 gggcagatga cggatgaagt aaaagccgcg accagcacgc agattttcag catcaaccag
    15121 acggataaag gccaggggca cattattatt gattacccgc gcctgctgaa tcacgggctg
    15181 ggtgagctgg tagcgcagat gcagcaacat tgtcagcaac agccggagaa tcacttttat
    15241 caggcagcgc tgttactgct cgaagcctcg cagaaacaca ttttgcgtta cgccgaactg
    15301 gcggaaacga tggcggcaag ctgcactgat gcccagcgtc gcgaagaact gctgactatt
    15361 gcggagatct cccgccataa cgccgaacat aagccgcaga cgttctggca ggcgtgccag
    15421 ttattctggt acatgaacat cattctgcaa tacgaatcca acgccagttc actatcgttg
    15481 gggcgcttcg accagtatat gttgccgttc tatcaggcat cattaaccca gggcgaagat
    15541 ccggcgttcc tgaaagaact gctcgaatct ttatgggtga aatgcaacga catcgtgctg
    15601 ttgcgctcca ccagtagcgc gcgttatttc gccggtttcc cgaccggcta taccgcactg
    15661 ctcggcgggt taaccgagaa cggacgtagc gcggtgaacg tgctttcgtt cctttgcctt
    15721 gacgcttatc aaagcgtgca attaccgcaa ccgaacctcg gcgtgcgcac taacgccttg
    15781 atcgacacgc cgttcctgat gaaaaccgca gaaaccattc gcctcggcac cggtattccg
    15841 caaatcttta acgatgaagt ggtggtgcca gcgttcctca accgtggcgt ttcgctggaa
    15901 gatgcacgcg actattccgt agtgggctgt gtggaattat ctattcccgg cagaacctac
    15961 ggcttgcatg acatcgcgat gtttaatctg ctgaaagtga tggaaatctg cctgcatgaa
    16021 aatgaaggca atgctgcgct gacttatgaa ggtttactgg agcagatccg cgccaagatc
    16081 agccactaca tcaccctgat ggttgagggc agcaatattt gcgatatcgg tcaccgtgac
    16141 tgggcacctg taccgctgct ctcatcgttt atcagcgatt gtctggaaaa aggcagcgat
    16201 attaccgatg gcggcgcgcg ttataacttc tccggcgtac aggggatcgg tatcgccaac
    16261 ctgagcgatt ctctccatgc gttgaaaggg atggtttttg agcaacagcg tttaagtttt
    16321 gacgaattgc tgtcggtatt aaaagccaac ttcgcaacgc cagaaggcga aaaagtccgt
    16381 gctcgcttaa ttaaccgctt cgagaaatac ggtaacgata tcgacgaggt ggataacatc
    16441 agcgccgaac tgttacgcca ctactgcaaa gaagtggaaa aataccagaa cccgcgcagc
    16501 ggctacttca cgccgggatc gtataccgtt tctgctcacg tcccgttggg atcggtggtt
    16561 ggcgcgacgc cagacggtcg ttttgccggg gaacaactgg cagacggcgg cttgtcaccc
    16621 atgctgggcc aggacgcaca agggccaacg gcggtactga agtcagtcag taagctcgat
    16681 aatacgctgc tgtctaacgg tacgttgctg aacgtgaaat tcactccggc gactctggaa
    16741 ggtgaagcgg gattacgcaa actggccgac ttcttacggg cgtttaccca gcttaagttg
    16801 cagcatattc agtttaacgt ggtgaacgcc gacacgttgc gggaagcgca acagcgccca
    16861 caagattatg ccgggctggt ggtgcgcgtt gccggataca gcgccttctt tgtcgaattg
    16921 tcgaaggaga tccaggatga catcatccgc cggacagcgc atcagctgta acgttgtgga
    16981 aacgcgccgc aatgatgtgg cgcgcatttt caacattcag cgttattcac tgaatgacgg
    17041 cgagggcatt cgtacggtgg tcttttttaa aggctgtccg catctttgcc catggtgtgc
    17101 taatccggag tcgatctccg gcaaaatcca gacggtacgc agagaggcga aatgtctgca
    17161 ctgtgcgaaa tgtttgcgtg atgcggatga atgcccctcc ggggcgtttg aacggattgg
    17221 ccgcgatatc agccttgacg cgctggagcg ggaagtgatg aaagatgaca ttttctttcg
    17281 cacgtccggc ggcggcgtca cgctttctgg cggcgaagtg ttaatgcagg cggagtttgc
    17341 tacccgtttt ttacagcggc tgcggttgtg gggcgtgtca tgcgccattg aaactgccgg
    17401 agacgcgcca gccagcaagc tgttaccgct ggcgaaattg tgcgatgaag tgttgttcga
    17461 tttaaaaatt atggacgcga ctcaggcgcg ggatgtggtg aagatgaatc tgccacgcgt
    17521 gctggagaat ctgcgtttgc tggtgagtga gggcgtcaac gtgatcccgc gtttaccgct
    17581 gatccctggt ttcacgctca gccaggagaa tatgcagcag gctctggacg tgctgatccc
    17641 gctgaatatc aggcagatcc atctgttacc gtttcatcag tacggcgaac cgaaataccg
    17701 cctgctgggg aaaacatggt cgatgaaaga ggtgcctgcg ccgtcgtcag ctgatgtggc
    17761 aacgatgcgc gaaatggcag aacgggccgg atttcaggtt accgtgggag gttaaaatgg
    17821 catacctggt ggcagtaacc gcctgcgtca gtggcgtggc gcatacttat atggcggcgg
    17881 aacggctgga aaagttgtgc ctgttagaga agtggggcgt cagcattgaa actcagggcg
    17941 cgctgggaac ggagaatcgt ttagcggacg aggatatccg ccgggcggat gttgcgctgt
    18001 taattacgga tatcgagctt gccggtgctg agcgatttga ccattgccgc tatgtgcaat
    18061 gcagcatcta cgctttcctt cgtgagccgc agcgggtgat gagcgcggta cgcaaagtgc
    18121 tttctgcgcc gcagcaaacc catctcattc tggagtagtc ggtttttctg tcagttggct
    18181 gtggtactgc cgacgatatt ccgacggtga gcgttcggta tttttacgaa acagacggca
    18241 gaagtagttg ctgtcgacaa agccgcaggc gtgcgccact tcttttacct tcaggtcata
    18301 gcctttcaat aacgtcttag cgtgctccag tcgcgtgtga ttcaggtatt cgttaaaacc
    18361 agtggccccc gttttttgaa acaggtgcga gaggtaattt ggcgaaatat agaatgcctg
    18421 tgcgacagat tcgcgggtaa gcgcggaggc atagcgttcg tcgatataat cacgaatagc
    18481 ttcgaacagt gcctggctgc gtgaggcggt ctggatttgg ctgccaagca aatcgcggca
    18541 gtggctaagc aggctggcga cgataagcct tgccgtttgc tgctcctgcg actgcatttg
    18601 catttcattg agcgtttgta gcagaaaaga acctatgcgt gggccgcgtc gggcgacgtg
    18661 ttgcttcgcc agattttgat attgtttgcc atcccattgc acgacgctga agccgagttg
    18721 ctgtttgcca aacagcacgc taaaggtagt ggcgggggct tgccattgtg gaaaattcca
    18781 gccaccagcg ggaacgtaca gcacatcgcc gggaactaac gctgctccag cgccggtatc
    18841 aataaactca ccttccagca caatttccag ccgtggaaaa tcgacctgat acgcaagatc
    18901 gggcaccggg ccgttgctgc tggcaaagta aatctggcgc agggacagcg ggccgttgat
    18961 caggcgggag agcagatagc tgacgtcgtg atacatgtta ttcccttagg tatgtagacg
    19021 ggaaaacagt aactggcatt atgaaaaaag gccacctgaa aagtgtggcc tgatgggagt
    19081 gagatgtaaa cattgcggca gtttcagtcg acttgtattg ccggatgcgg cgtaaacgcc
    19141 ttatccggcc taccgccgcg atctgtaggc cggataagac gcgtcaagcg tcgcatccgg
    19201 caaataacat ctggctgata attactgatt acccacctga tcgccatacg gcagagtgtc
    19261 gtaatcgatc agtgcgtttt tcttatacgg gttaccaatc cagcgggtag tttctttgaa
    19321 ctgcggattc accactgaac gggttggatc gtaaccgtcg taagataagc ccgccaaatc
    19381 tgaccaggtg tggatcagtt ccgccaggct gtatttacga tcaacatcct gtgagaaatc
    19441 acgcggatga gtcgcttgcc atttttctga cgtccacagc aggaacggaa tggtgtacat
    19501 atgacgcgtc gggttgtctt cattacgccc ctgagttttg tgcggcggcg tgtcgtaaac
    19561 ctcttcaccg tggtcagaga aataaaccag gaaaccgttc ggatcggtcg ctttaaagtc
    19621 tttaatcagg ctggcaacca cgtgatcgtt gtacaggtta gcattgtcat aatcattata
    19681 tgactccagc tcttctgcgt ttaatcccgg tggaacatgc tctgtattac cgtcaaattt
    19741 gccctgatct tccggataac ggtacttgta cttgatatgc gtacccagca gatgaacgat
    19801 gatcagtttc ttcggcgcag ggtccttcag cacttcctgg aacggcttca gcacgttggt
    19861 gtcgtattca cgcgcactct gcgtacgttg ctggttcatg tagtactgct tatcggtctg
    19921 gcgcgaaaat accgtcagca tggtattgcg ggcggtcatc gtctgctggt tggtgatcca
    19981 gaaggtttta taacccgcct gtttcatcat gttcatcagc gacggctgcg tcagatacag
    20041 atccgggttc ttttcattgg caaaggtcag cgcctgttgc aggatttcaa tggtgtacgg
    20101 acgagacgta accacgttat taaacacggt cagattcgga tcggttttat gcagcgcatc
    20161 cagctccggc gtggtttcac gcggataacc gtacagactc atgcgtccac gctgggtcga
    20221 ttcgccaatt accagcacta aggtgcgcgg ttcgttaccc gattcatctt tgaaattcgc
    20281 cagcggcggc aaggcattat tttcattcag tagctttgtt agcgagttta gttgctgacg
    20341 atactgataa tagccggtca ggaattgcca cggtgcggca ggctccatac gcgatgccag
    20401 gttatccaac gttttctcaa atggcttgtt tttgataaac gtattcatgg cgatcggatg
    20461 cagaatcaag ccataaagta gggcaaaaga gacaacataa cgccacggct ttggaatata
    20521 gaccgggcgc aggcgcgtcc acagcaaaac tgccaccgcc gtataggcca gcgcgataag
    20581 cacaatcttc aggctgaaat actggcttaa atactcgctg gcttcgttgg tgttggtttc
    20641 aaacatcaca aacagaacgc tctgcgagaa ctcctgaccg tagatgacgt agtagcacaa
    20701 cgctgccaga gaggccgccc atagcaccac gccgatcact gcggcaataa ttttaatccg
    20761 cttcggaaag aggaataccg ggatcaacca cagcgaactg aataacagcg agtcgcgaat
    20821 gccgttagtg ccactataac cactgatgta aataatggcc tgtagcagag tagagaaaaa
    20881 ccaaaagtag agcagtgccc aacccagggc cttccagcta aaaaggggtt tagcctggac
    20941 ttctgtggaa tgcatagtaa gaacctgtct tgaaaaaaat atcgccgaat gtaacgacaa
    21001 ttccttaagg atatctgaag ggatatccag attttgaata aaatgcagac agaaatatat
    21061 tgaaaacgag ggtgttagaa cagaagtatt tcagaaaacc ctcgcgcaaa agcacgaggg
    21121 tttgcaggag aggaagatta gccggtatta cgcatacctg ccgcaatccc ggcaatagtg
    21181 accattaacg cctgctcgac gcgaggatcc ggctcatggc cttctttttc tgcctggcgg
    21241 gagcggtgca gcaactcggc ctgcaatacg ttcagcgggt cggtgtaaat attccgtagc
    21301 tgaatagact ctgcaatcca cggcagatcg gccatcaggt gagaatcgtt ggcaatcgcc
    21361 agcaccactt tgatgtcttc ttcttgcaga ttgcgtaact ctttacctaa cggccacagt
    21421 gctttgtcta ccaggcgttg gtcatagtat tccgccagcc acaggtctgc tttggcgaag
    21481 accatctcca gcatgccgag acgcgtcgag aagaatggcc aatcgcggca catggcttcc
    21541 agctcgctct gtttgccatc ttcgaccact ttttgcagcg ccgtacctgc acccagccag
    21601 gcggggagca tcaggcggtt ttgtgtccag gcaaaaatcc acggaatagc gcgcagtgac
    21661 tcaacgccac cggtcgggcg acgttttgcc ggacgtgaac ccagcggtag tttgccgagt
    21721 tcctgctccg gcgtggcaga gcggaagtaa ggcacaaaat ctttgttttc acgcacgtag
    21781 ccgcggtaga catcgcatga gataaccgaa agctcatcca taatgcgacg ccagctctct
    21841 ttcggctctg gcggtggcag caggttggct tccagaatcg ccccggtata cagcgaaagg
    21901 ctgctaacgg tgatttcagg cagaccgtac ttgaagcgga tcatctcgcc ctgttcggtc
    21961 acgcgcaggc cgcctttcag gcttcctggt ggttgtgaca gcagcgccgc atgggcaggt
    22021 gcgccgccgc gaccaatgga accgccgcga ccgtggaaca acgttagctc aatacccgct
    22081 ttttcgcagg ttttgattaa tgcatcctgt gcctgatatt gcgcccagga agctgccatc
    22141 acgcccgcat cttttgctga gtcggaatag ccaatcatca ccatctgttt gccctgaatc
    22201 aggccgcgat accagtcgat attcagcagc tgggtcatga catcgttggc gttgttcagg
    22261 tcatcgaggg tttcaaacag cggagcaacc ggcatcgcaa acccgatacc cgcttctttc
    22321 agcagcaggt ggacagccag cacgtcggac ggcgttttcg ccatcgagat cacgtaggcg
    22381 gcaatggagc cttgcggtgc ttcggcaatc acctggcagg tatcgagcac ttcgcgcgtt
    22441 tcggcgcttg gttgccagtt gcgcggtaga agcggacgtt tggagttcag ttcgcggatc
    22501 aggaacgcct gtttgtcggc ttctgaccag ctctcgtagt cgccgatacc gaggtagcgg
    22561 gtcagctcgc ccagcgcttc ggtatggcgg gtgctctcct gacggatatc aatacggacc
    22621 agcggtacgc cgaaacattt cacgcggcgc agggtgtcga gcagatcgcc gttggcgata
    22681 atacccatgc cacacgcctg aagtgactgg tagcaagcgt agagcggttc ccacagttct
    22741 tcgttttgtg tcagcaggcc ttctggtttt ggcagttctt cgcctttcag gcgcgcttcc
    22801 agccatgcct gtgtcgccat caggcgagaa cgcaggtttt tcatcagata gcgatacggt
    22861 tctgcggcac cttcttcgcc aaccagcgcc agcagttcag gggtcgcttc aaccatcgac
    22921 agttcagaaa ccagcacctg aatatctttc aggaacagat cggtggcttt ccagcgactg
    22981 agcagcagga cgtggcgggt gatatcggca gtgacgttcg ggttgccgtc gcggtcaccg
    23041 cccatccacg aggtaaaacg gaccggaaca aattcgacgg gcagtttgta gccgagattc
    23101 tcttccagtt gttcgttcag ttcgcgcagg taattcggta cgccttgcca caggctgttt
    23161 tctactacgg caaagcccca tttggcttca tctaccgggc ttggacgcag cttacggatt
    23221 tcatctgtat gccatgactg ggcgatcaac tggcgcaggc ggcgcatcag ctggttatgt
    23281 tcgtagtcgg cgatatcttt gttatcgagc tgttttaagc aggcgttcac ttccaccatt
    23341 ttgtggatca gtgtacgacg ggtaatttcg gttgggtgag ccgtgaggac cagttccagc
    23401 gacagcgatt ccactgcttt tttgatggtg tcttcgctca gttccggctg gtttttcagt
    23461 ttacgcaggg tgcgggcgat cacttccggg ttgctggcag cttcgccttt cggcgaaatg
    23521 ctgtggtatt gctcagcggt gttggccagg ttcaggaact gactaaacgc acgcgcaacg
    23581 ggcagcagct cgtcgttcga caaattttgt aaggtggtga gcaactcctg gcggttagca
    23641 tcattgccag cgcgtgaaga tttcgacaac ttacggatag tttctacgcg ttcaagaatg
    23701 tgttctccca acgcatcctt gatggtttct cccagcactt tgccgagcat actgacatta
    23761 ctacgcaatg cggaatattg ttcgttcata ttaccccaga caccccatct tatcgtttga
    23821 tagccctgta tccttcacgt cgcattggcg cgaatatgct cgggctttgc ttttcgtcgt
    23881 cttttataaa gccacgtaaa agcggtgacg tcaaatgctg cgaaatcgct tcagcaaacg
    23941 aataaatagc aggaatttac gccattaaat tcatgacgct ttaaataagc gtaacttatg
    24001 ggaatgttaa aaaatcgcct cgagtaacac caaaggtgta ggtcggataa gatgcgtaag
    24061 catcgcatcc gacattgttt ggcctacgtt ttaatgccag caaaaatggt gaatcacctg
    24121 ggtgatcagt tcgcgggtgg gcttgataaa cctcgtctcc agatattcat caggctgatg
    24181 agcctgatta attgagccag gccccagaac cagcgtcggg cataacgttt gaataaacgg
    24241 cgcttcggta cagtaattca ccacttcggt ctttgctccg agcaatttct caaccacttc
    24301 aaccagttta tgattcggtg ggcattcata gccagggatc ggcggatgca gctcgtcgac
    24361 cgtcagacga cccggccagc gttcgctcac tggagccaat gcatcgttga gcaaaccatt
    24421 aagttcattg agtgtcatgc ctggcagcgg acgaatatcc atatgcaact cacagcaagc
    24481 acaaatacgg ttagaagcgt cgccaccgtg gatatgcccg aggttgagcg tagggtatgg
    24541 cacggtaaac gcgtcgtagt gataacgttc tttcaggtta tcgcgcaatt gcaaaatatg
    24601 cccgatggcg tcgtgcatca gttcgatagc gttaactccg cgtgctggat cgctggagtg
    24661 gcccgactgg ccctgaatac ggatggcgtt agagatatga cctttatgcg cgcgtaccgg
    24721 ttgcagcgac gttggctcgc caatgatggc gcaatccggg cgcagggcgg tagtttcggc
    24781 aaaataacgc gctccggcca tactggtttc ttcatcggca gtcgccagaa tgtagagcgg
    24841 ttttgcaagc ttcgtgacgt cgacatcgcg tagcgcatca aggataaacg caaaaaagcc
    24901 tttcatgtcg gcggtgccta agccgtaaag cttgccgtca tgctccgtca acgtaaatgg
    24961 atcgcgcgtc cagcgtccgt catcaaatgg caccgtatcg gtatgccccg ccagcaacaa
    25021 accgccagcc ccctgtccgc aactggccag catattaaat ttgttgcgag ttcctggcac
    25081 aggctgtact tccacattga agcctaaatc cttaaaccag tccgccagca gagtgattaa
    25141 atctgcattg ctttgatcga gtgcctcttc cgtggcgctt attgaaggtg tggcaatcag
    25201 agcacggtaa atctcgataa atggcggtaa tttgtttttc attgttgaca cacctctggt
    25261 catgatagta tcaatattca tgcagtattt atgaataaaa atacactaac gttgagcgta
    25321 ataaaaccca ccagccgtaa ggtgaatgtt ttacgtttaa cctggcaacc agacataaga
    25381 aggtgaatag ccccgatgtt gaatacgctg attgtgggtg ccagcggcta cgctggcgca
    25441 gagctagtga cctatgtaaa tcgccatccg catatgaaca taaccgcttt gactgtttca
    25501 gcgcaaagca atgatgcggg aaagttaatc tccgatttgc atccgcagct aaaaggcatc
    25561 gttgatctgc cgttgcagcc gatgtcggat atcagcgagt ttagcccagg ggtggacgtg
    25621 gtgtttctcg ccaccgccca tgaagtcagc cacgatttag cgccgcaatt tcttgaagca
    25681 ggctgtgtgg tgttcgacct ttccggcgcg tttcgtgtta acgatgtcgc cttctatgaa
    25741 aaatattacg gctttaccca tcaatacccg gaactgctgg aacaggcagc ctacggtctg
    25801 gcggagtggt gcggtaataa attaaaagaa gcgaacttga ttgccgtgcc aggctgttat
    25861 ccgacggcag cacagctggc gctgaaaccg ttgattgatg ccgatcttct tgacctcaat
    25921 cagtggccgg tgatcaacgc caccagcggc gtgagcggtg caggacgtaa ggcagcgatt
    25981 tcaaatagtt tttgtgaagt tagcctgcaa ccgtatggcg tctttaccca tcgccatcaa
    26041 ccagagatcg ccacacacct cggtgctgac gttatcttca ccccgcatct gggcaatttc
    26101 ccgcgcggca ttctcgaaac cattacctgc cgcctgaaac cgggtgtgag ccaggtgcaa
    26161 gtggcgcaag cgttacagca ggcgtatgcc cataaaccac tggtgcggct gtatgacaaa
    26221 ggcgttccgg cgctgaaaaa tgtcgttgga ctgccattct gcgatatcgg ttttgccgtt
    26281 cagggcgagc atctgattat tgtggcgacc gaagacaact tactgaaagg cgcggcggca
    26341 caagcggtgc agtgcgccaa tattcgtttc ggctatgcgg aaacgcagtc tcttatttaa
    26401 gggtgcaatg atgaatccat taattatcaa actgggcggc gtactgctgg atagtgagga
    26461 ggcgctggaa cgtctgttta gtgcactggt gaattatcgt gagtcacatc agcgtccgct
    26521 ggtgattgtc cacggtggcg gctgcgtagt ggatgagttg atgaaagggc tgaatctgcc
    26581 ggtgaaaaag aaaaatggcc tgcgggtgac gcctgctgat cagattgaca ttatcaccgg
    26641 agcactggcg ggaacggcaa ataaaaccct gctggcatgg gcgaagaaac atcagattgc
    26701 ggccgtaggt ttgtttctcg gtgacggcga cagcgtcaaa gtgacccagc ttgatgaaga
    26761 gttaggccat gttggactgg cgcagccagg ttcgcctaag cttatcaact cattgctgga
    26821 gaacggttat ctgccggtgg tcagctccat tggcgtaaca gacgaagggc aactgatgaa
    26881 cgtcaatgcc gatcaggcgg caacggcgct ggcggcaacg ctgggcgcgg atctgatttt
    26941 gctctccgac gtcagtggca ttctcgacgg caaagggcaa cgtattgccg aaatgaccgc
    27001 cgcgaaagca gaacaactga ttgagcaggg tattattact gacggcatga tagtgaaagt
    27061 gaacgcggcg ctggatgctg cgcgcacgtt gggccgtccg gtagatatcg cctcctggcg
    27121 tcatgcggaa cagcttccgg cactgtttaa cggtatgccg atgggtacgc ggattttagc
    27181 ttaagttttg ttggccggag gcgcagcttt ccggcattga atttcaaaat aaggaaacag
    27241 agttatggca ctttggggcg ggcgttttac ccaggcagca gatcaacggt tcaaacaatt
    27301 caacgactca ctgcgctttg attaccgtct ggcggagcag gatattgttg gctctgtggc
    27361 ctggtccaaa gccctggtaa cggtcggcgt gttaaccgca gaagagcagg cgcaactgga
    27421 agaggcgctg aacgtgctgc tggaagatgt tcgcgccagg ccacaacaaa tccttgaaag
    27481 cgacgccgaa gatatccata gctgggtgga aagcaaactg atcgacaaag tgggtcagtt
    27541 aggcaaaaaa ctgcataccg ggcgtagccg taatgatcag gtagcaactg acctgaaact
    27601 gtggtgcaaa gataccgtta gcgagttgct gacggctaac cggcagttac aatccgctct
    27661 ggtcgagacc gcgcagaaca atcaggacgc ggtgatgccg ggttacactc acctgcaacg
    27721 cgcccagccg gtgacgttcg ctcactggtg cctggcctac gttgagatgc tggcgcgtga
    27781 tgaaagccgt ttgcaggacg cgcttaagcg tctggatgtc agcccgctag gctgtggcgc
    27841 actggcagga acggcctatg aaatcgaccg tgaacagtta gcaggctggc tgggctttgc
    27901 ttcggcgacc cgtaacagtc tcgacagcgt ttctgaccgt gaccatgtgc tggaactgct
    27961 ttctgctgcc gctatcggca tggtgcatct gtcgcgtttt gctgaagatc tgattttctt
    28021 taacaccggc gaagcggggt ttgtggagct ttctgaccgt gtgacttccg gttcatcatt
    28081 aatgccgcag aagaaaaacc cggatgcgct ggagctgatt cgcggtaaat gcggtcgggt
    28141 gcagggggcg ttaaccggca tgatgatgac gctgaaaggt ttgccgctgg cttacaacaa
    28201 agatatgcag gaagacaaag aaggtctgtt cgacgcgctc gatacctggc tggactgcct
    28261 gcatatggcg gcgctggtgc tggacggcat tcaggtgaaa cgtccgcgtt gccaggaagc
    28321 ggcgcagcag ggttacgcca acgctactga actggcggat tatctggtgg cgaaaggcgt
    28381 accgttccgc gaggcgcacc atattgtggg tgaagctgtg gtggaagcca ttcgtcaggg
    28441 caaagcgttg gaagatctgc cgctcagtga gttgcagaaa ttcagccagg tgattggcga
    28501 agatgtctat ccgattctgt cgctgcaatc gtgcctcgac aagcgtgctg caaaaggcgg
    28561 cgtctcaccg cagcaggtgg cgcaggcgat tgcttttgcg caagctcggt tagagtagtt
    28621 tgtttatatt gctcccctga aaatggggag cttaccacgc ctacaaagtg cgacacttcg
    28681 taggcggata aggcgtttac gccgcatcag gcagaaattt gtcagtattc tggtgacctt
    28741 aaatttaacg cccaaccaga accattattt gtcacattcc tttttattct tgcgtcttct
    28801 ttaccttcac tctccccata aaaattatat tttcaataca tcagccaata catactttaa
    28861 ttaattgaat ataattcatt tctattgctg tgttcatttt taatgattaa tggagtttgt
    28921 tatgtgatta tttaacgtgt tgttttaacg atgatagcta attattcagg tggtttttga
    28981 gggtgttttt tctcctcggt agaaattttt atttttaatg tgatcatggt tgaataatcg
    29041 ctctttctct cttgtatggt agtaggcgca aaattaagtt tgaaacgtct atttacaaca
    29101 caggagattg caataatgaa aattattgct gcagatgttt ttgttacttg cccagggcgt
    29161 aatttcgtta cgcttaaaat aaccactgca agcggacttt gtggattagg agatgccacg
    29221 ttaaatggtc gcgaattgtc ggtcgcttct tatctgaaag atcatctttg tccacaacta
    29281 attggccgcg atgccagccg tatcgaagat atctggcaat tcttttataa aggtgcttac
    29341 tggcgacgtg gccccgtcac gatgtcagcc atttctgcca ttgatatggc gttgtgggat
    29401 atcaaagcaa aagcggcaaa tatgccattg tatcaactgc ttggtggtgc ttctcgtgaa
    29461 ggcgttatgg tttattgcca caccaccggg cgtacgattg atgaagtcct tgaagactat
    29521 gcaaagcacc agcagatggg ctttaaggcg attcgtgtgc agtgtggtgt accgggtatg
    29581 caaaccacct atggtctggc gaagggcaaa ggactggcgt atgaaccagc aaccaaaggc
    29641 ctgtggccag aagagcagtt gtggtcttca gaaaagtatc tcgattttac gccgaagtta
    29701 tttgaagcgg tgcgtaataa gttcggtttt aacgaacatc tgttgcatga catgcaccat
    29761 cgcctgaccc ccatcgaagc ggcgcgcttt gggaagagca ttgaagatta ccgcctgttc
    29821 tggatggaag atccgacgcc tgccgagaat caggaatgtt ttcgtctgat tcgtcagcat
    29881 accgtgacgc cgattgcggt cggtgaagtg tttaacagta tctgggactg caagcagtta
    29941 atcgaagagc agctgattga ctatattcgc acgacgatta cccatgcagg cggcattacc
    30001 gggatgcgac ggattgccga ttttgcatcg ctttatcagg tacgtaccgg ttcgcatggc
    30061 ccttcagatt tatcgcctgt ttgccatgcg gcggcgctgc attttgacct ctgggtgcca
    30121 aactttggcg ttcaggagta catgggctat tccgaacaaa tgcttgaagt cttcccgcat
    30181 agctggcgct tcgatgaagg ttatatgcac ccaggcgatg aaccggggct gggtatttct
    30241 ttcgatgaga aactggcggc gaaatatccg tatgacccgg cgtatctccc cgtcgcacga
    30301 ctggaagatg gcacattgtg gaactggtaa tagcggttcg cttaattatt ttctgaatat
    30361 tcatcaaatc gattaattcc gctcaaggag ctaaaaatgg acgttgatgt gtcaaccagt
    30421 gttgcgggta ataaaccgca acggattcgt cgtattcaga cagtgaccct ggttttatta
    30481 tttatggcgg ggatcgttaa ttttctcgac cgttcgtcat tgagcgtggc aggtgaagca
    30541 attcgtggcg agttaggatt atccgctacc gagtttggtg ttttgctttc tgcattttct
    30601 ctgtcttatg gtttttcaca acttccttcc ggtattttgc tagaccgctt tggcccacga
    30661 atagtgttag gcgcaggctt aatattctgg tcattaatgc aggcattaac aggaatggtt
    30721 aattctttta gccactttat tttaatgcgt atcggtctgg gtattggcga agcgccattt
    30781 atgcctgcgg gagtaaagtc gatcaccgac tggtatgcgc aaaaagaacg cggcacggcg
    30841 ctggggattt ttaactcatc taccgttatc ggtcaggcta tcgcgcctcc tgctcttgta
    30901 ttgatgcaac tggcatgggg ctggcggacg atgtttgtca tcatcggcgt agcagggatt
    30961 ctggttggga tctgttggta cgcgtggtat cgcaaccggg cgcagtttgt cctgaccgag
    31021 gaagaacgga catatctctc cgcgccggtt aaaccgcgtc cacagttaca atttagcgag
    31081 tggctggcgc tgtttaagca tcggacaacc tgggggatga ttttaggttt ctctggcgtc
    31141 aattataccg gttggctcta catcgcgtgg ttacccggtt atttgcaggc cgaacaaggt
    31201 ttcagtctgg cgaaaaccgg ttgggtggcg gcaattcctt tcctcgcggc ggcggtcggg
    31261 atgtgggtta acggtattgt tgtcgatcgg ctggcgaaaa aaggctatga cctggcgaag
    31321 acgcgtaaaa cggcgattgt ctgcggtttg atgatgtcgg cattaggcac gttgctggtc
    31381 gtgcaatctt cctcgccagc ccaggcggta gcgtttatct caatggcgct gttctgcgtg
    31441 catttcgcgg gaacatctgc atgggggctg gtgcaggtga tggtgtcaga aacaaaagtg
    31501 gcttccatcg ccgggattca aaactttggc agttttgttt ttgcttcctt tgctccgatc
    31561 gtaaccggtt gggtcgtgga taccacacac tcgtttaatc tggcgctggt tatcgcggcc
    31621 tgtgtgacgt tcaccggggc gctgtgttac ttctttattg tcaaagatcg gattgagtaa
    31681 ccgttactat caggctctct tgctgtgggc ctgtagaata aaaaaaagcg gatcctggag
    31741 atccgcaaaa gttcacgttg gctttagtta ttcgagttga gaaactctcg aaacgggcag
    31801 tgacttcaag ggttaaaaga ggtgccgctc cgtttctgtg agcaattatc agtcagaatg
    31861 cttgataggg ataatcgttc gttgctatgc tacctatcgc cgcgaactat cgtggcaatg
    31921 gaggatggat aatgaatatt cgtgatcttg agtacctggt ggcattggcc gaacaccgcc
    31981 attttcggcg tgcggcagat tcctgccacg ttagccagcc gacgcttagc gggcaaattc
    32041 gtaagctgga agatgagttg ggtgtgatgt tgctggagcg gaccagccgt aaagtgttat
    32101 tcacccaggc gggaatgctg ctggtggatc aggcgcgtac cgtgctgcgt gaggtgaaag
    32161 tccttaaaga gatggcaagc cagcagggcg agacgatgtc cggaccgctg catattggtc
    32221 tgattcccac ggttggaccg tacctgctac cgcatattat cccgatgctg caccagacct
    32281 ttccaaagct ggaaatgtat ctgcatgaag cacagaccca ccagttactg gcgcaactgg
    32341 acagcggcaa actcgattgc gtgatcctcg cgctggtgaa agagagcgaa gcattcattg
    32401 aagtgccgtt gtttgatgag ccaatgttgc tggctatcta tgaagaccat ccgtgggcga
    32461 accgcgaatg cgtaccgatg gccgatctgg caggggaaaa actgctgatg ctggaagatg
    32521 gtcactgttt gcgcgatcag gcaatgggtt tctgttttga agccggggcg gatgaagata
    32581 cacacttccg cgcgaccagc ctggaaactc tgcgcaacat ggtggcggca ggtagcggga
    32641 tcactttact gcctgcgctg gctgtgccgc cggaacgcaa acgtgatggg gttgtttatc
    32701 tgccatgcat taagccggaa ccacgccgca ctattggcct ggtttatcgt cctggctcac
    32761 cactgcgcag ccgctatgag cagctggcag aggccatccg cgcaagaatg gatggccatt
    32821 tcgataaagt gttaaaacag gcggtttaag ccatttagcg cagctacccg ataggcttcc
    32881 gccatcgtcg ggtagttaaa ggtggtgttg acgaagtact caatagtgtt gccgccacct
    32941 ttctgttcca taatcgcctg accgatatga ataatttcgg cagcgcgctc gccaaagcag
    33001 tgaataccca gaatctcttt tgtttcccga tggaacaaaa ttttcagcgt gcccacgttc
    33061 atgccgacga tttgtgcacg tgccagatgt ttaaactggg cgcggcccac ttcatatggc
    33121 actttcatcg cggtcagctg ctgttcggtt ttgcccacag agctgatttc cgggatggtg
    33181 taaataccgg tagggatatc ttcaatcaga tgtgcggtgg cttcaccttt caccagcgcc
    33241 tgcgcggcaa tgcgcccctg gtcataggcc gccgacgcca ggctcggata accaatcacg
    33301 tcgcccaccg cgtaaacgtg cggctgtgcg gtctgataca tgctgttgac cttcagctgt
    33361 ccgcggctgt cagtttccag cccaatgttc tgtaacgcca gcgaatcggt attaccggtg
    33421 cgaccgttgg catagagcag gcagtcagct ttcagttttt tacccgactt caaatgcatg
    33481 atcacaccgt cgtcacagcc ttcgatcttc tcgtactctt cgttgtgacg aatcactacg
    33541 ccactgttcc agaagtgata ggagagagaa tctgacatct cttgatcgag aaatgccagc
    33601 aggcgatcgc gggtgttgat cagatccact tttacatcca taccgcggaa gatcgacgca
    33661 tattcacagc cgatcactcc agcaccatag ataagtacat ggcgcggttc gtggtgcatg
    33721 ctaagaattg agtcgctgtc gtaaatgcgt ggatgcgtga aatcaacatc tgttggatga
    33781 tatggacgag agccgcaagc aataacaaat ttttcagcgg ttagtgtctc gacgctgccg
    33841 tccgggcaat ccagcgccag cgtgtgctcg tcaacaaagc gagcgttccc ctgcaatatt
    33901 tcacagtgat tacgttcgta aaatccctga cgcatgcgcg tttgttgatt aatcacgtta
    33961 tcggcatggt taaggatatc ggcaaaagaa gagcggagca gtcgcgaatg gtcgctgtaa
    34021 agtgggtttt gattaaattc tataatgcgg ctgacggcgt gacggagagc tttcgacggg
    34081 atggtgcccc agtgggtgca accgccgcca acattttgat aacgctcgat aactgcgacg
    34141 cgcgcacctt gcttaaccag gcccattgca gcgccttcgc cgccggggcc ggaacctatt
    34201 actatggcat cgtaatcgta ggaatgtggc atggtagggc ttacctgttc ttatacataa
    34261 aagcaacaga atggtaacat tttatcgcgg gtaagccaat tgatccccgt catttatctg
    34321 gctatatcgt gagcggcctt tgctttgtct gtttcttact tttgacctga acgttttatt
    34381 ggatttttat cgacgatact ctccgtttaa gcggcaggtt tccgttgtac gtaaaagaac
    34441 cggccaaaga attgcagtaa atatgtttta ttgcgttacc gttcattcac aatactggag
    34501 caatccagta tgttcattct ctggtatagt gccagcagta cttttggcaa ggattcagac
    34561 atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc gccgttcgct ggtggaagcc
    34621 gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc tgagtttgcg tgaagtggcg
    34681 cgtgaagcgg gcattgctcc cacctctttt tatcggcatt tccgcgacgt agacgaactg
    34741 ggtctgacca tggttgatga gagcggttta atgctacgcc aactcatgcg tcaggcgcgt
    34801 cagcgtatcg ccaaaggcgg gagtgtgatc cgcacctcgg tctccacatt tatggagttc
    34861 atcggtaata atcctaacgc cttccggtta ttattgcggg aacgctccgg cacctccgct
    34921 gcgtttcgtg ccgccgttgc gcgtgaaatt cagcacttca ttgcggaact tgcggactat
    34981 ctggaactcg aaaaccatat gccgcgtgcg tttactgaag cgcaagccga agcaatggtg
    35041 acgattgtct tcagtgcggg ggccgaggcg ttggatgtcg gcgtcgaaca acgtcggcaa
    35101 ttagaagagc gactggtact gcaactgcga atgatttcta aaggggctta ttactggtat
    35161 cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg tgaaggacga gtaatgaaac
    35221 aagcaaatca agatagaggt acgctgctgc tggcgttggt tgctggctta tcgattaatg
    35281 gtactttcgc agcgctgttt agctccattg tgccattttc tgtattcccg attatttccc
    35341 tgttgctgac ggtttactgc ctgcatcaac gttatcttaa tcgcaccatg ccggtaggct
    35401 tgccgggtct ggcagctgcc tgttttattc tcggcgtact gctgtacagc acggtagttc
    35461 gtgcggaata tccggatatc ggctctaact tcttcccggc agtactctcc gtcattatgg
    35521 tgttctggat tggcgcgaag atgcgtaacc gtaagcagga agttgctgag taatcggtat
    35581 catgccggat gctggtgcat ccggcatggg ttttacttcg cggtcagtaa tacgccgcac
    35641 tccatatggt gcgtgtaggg gaactgatca aacagagcca gacgttcgac cttgtgcgtc
    35701 tggcttaatg tttccagatt cttgcataac gtttccggat tgcaggagat atacagaata
    35761 cgtggatacg cctgcaccat tttctcggtt tcactgtcca gaccgctgcg cggtgggtcg
    35821 acaaaaatgg tttcgcactg ataactcttt aagtcgattc cttgcaggcg gttaaactcg
    35881 cgtacgccat tcatcgcctg agtaaattct tctgccgcca tacgaataat ttgtacgtta
    35941 tcaatatggt tagcggcaat gttgtattgc gcggcagcga ccgacggctt agcgatttcg
    36001 gtggctaata cccgatcaaa attgcgcgcc agtgctaatg aaaagttacc gttgccgcag
    36061 tacagctcca gcaaatcgcc ttttgagcct ttggttacgt ccagcgccca ttccagcatc
    36121 tgaatattca tcgccgcgtt cggctgggta aagctgtttt ctacctgacg gtagatcatc
    36181 tctttccctg cgaccggcag acgttcatcg atgtaatcct ggtccagctc gattttggtt
    36241 ttcgttgccc gaccaatcag atgcacattc agattctgcg ctcgcagtgc atcgcgcagg
    36301 gcttccgctt cctgacgcca ctcatcatcc agcttcttat ggtacagcag ggaaaccacc
    36361 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
    36421 ttattacgca cacccgcaat catcgccgtc atcaactggt tgataagttc actggcggcg
    36481 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
    36541 tcatcgccat cgtgccagat gcggaactcc gcacgcatcc ggtaatgact gaccggcgag
    36601 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
    36661 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
    36721 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
    36781 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
    36841 tgccggtcct gtgagttaat agggaatcca gtgcgaatct ggagctgacg cgcagcggta
    36901 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
    36961 ttagtatctt agatacccct ccaagcccga agacctgccg gctaacgtcg catctggttc
    37021 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
    37081 aatgattaaa aaagcttcgc tgctgacggc gtgttctgtc acagcctttt ccgcttgggc
    37141 acaggatacc agcccggata ctctcgttgt tactgctaac cgttttcagc agccagtaaa
    37201 tactgtcctt gcacctactg atattgttac ccgtgaggat attcaacgct ggcagtcgaa
    37261 agacctcaac gatgttatga gtcatctccc cggtgtaaat atctctcaaa ctggtggaat
    37321 ggggacgagt tcttctcttt atgttcgagg cactgaatcg cgccatgttt tagtgttgat
    37381 tgacggtgtg ccgatggccc gtgccggtat cgacaatgca attgatatta gccagttccc
    37441 ggtatcactt gtacagcgtg ttgaatatat tcgtgggccg cgttctgccg tttatggttc
    37501 tggtgccatc ggaggtgtgg tgaacattat tacaatgagt aatgatgaga aagctcagat
    37561 caacgcagga atgggtacaa atggttatca agcctatgat ggggtaataa ataaacgttt
    37621 tggcgatacg atggtaacag cagcaggtgc ttaccagacg accaaaggtt ttaacattca
    37681 gcctggctca ccatacagtg gtgatagcga tcgcgatgga tatcgcaaca aacttttctg
    37741 gggggggctg cagcataagt ttaacgataa cttctcgggg ttcttccgtg gttacagcta
    37801 cacagctaat aatgattacg accagggctc gatgtatggt tctgctactg gcaacgaaga
    37861 agctcagaat tacaaccagt cctgggatac tggcctacgc tataactccg gcatctattc
    37921 gtctcagtta attgctaact atcaacgact caagaattat aactacacaa ataatcttgg
    37981 tcgttatgcg ggagatgcca ccctggatga catggaacag cgttatattc agtggggtaa
    38041 tagcattgag gtagggcatg gtgcaattag cggcgggatc gattggaaac aggaaaaact
    38101 gacgtccagc agcattacaa aatcagatga ttacgaacgc gataccaccg ggttatacct
    38161 gactggacag caacagattt ccaatgtgac tttggaagct tctggccgag aagatcatga
    38221 tgaacagttt ggctggcatg gtacctggca gacagctgct ggctgggagt ttattgatgg
    38281 ctaccaggca acactttctt acggtaccag ttttctttcg ccttctcttg gacagcaata
    38341 cggggctgct cgctttgctt caatttatgg tcctggtatt gcagccaacc caaatttgaa
    38401 gccagaagaa tctaaacagt gggagattgg aattgagggg gtaactggga ctctggattg
    38461 gcggctctca ggatatcgtt atgaaattca gaacctgatt gattataaaa gcataaataa
    38521 tgtcaaccag tacattaatg tgaaatcagc cacgattaaa gggctggaat ggacgggaaa
    38581 tattacaacc ggtcctgttg aacatcgctt gacgttacaa tatgttgacc cgcgcgatga
    38641 tgaaacagat aaagtgctgt accgtcgggc aaaacagcag gttaagtacg agttgactgg
    38701 acaagcttat ggtttcgaat gggacgtatc ttaccaatat ataggtcaac gatacgacaa
    38761 cgcttatgac gagacctctg gctcatcaca tacagttaaa ttaggcggtg tgagcttgtg
    38821 ggatcttgcg gttgcgtatc cggtcacctc tcatctaaca gttcgtggta aaatagccaa
    38881 cctgttcgac aaagattatg agacagtcta tggctaccaa actgcaggac gggaatacac
    38941 cttgtctggc agctacacct tctgaaccac gtcccaccgt gctggtgttt gactccggcg
    39001 tcggtgggtt gtcggtctat gacgagatcc ggcatctctt accggatctc cattacattt
    39061 atgctttcga taacgtcgct ttcccgtatg gcgaaaaaag cgaagagttt attgttgagc
    39121 gagtggtggc aattgtcacc gcagtgcaag aacgttatcc ccttgcgctg gctgtggtcg
    39181 cttgcaacac cgccagtacc gtttcacttc ctgcattacg cgaaaagttc gacttcccgg
    39241 ttgttggtgt cgtgccggcg attaaacctg ctgcacgtct gacggcaaat ggcattgtcg
    39301 gattactggc aacccgcgga actgttaaac gttcttatac tcatgagctg atcgcgcgtt
    39361 tcgctaatga atgccagata gaaatgctgg gctcggcaga gatggtcgag ttggctgaag
    39421 cgaagctgca tggcgaagat gtttctctgg atgcgctaaa acgtatcttg cgtccgtggt
    39481 taagaatgaa agagccgcca gataccgttg tactggggtg tacccatttc cctctactac
    39541 aagaagaact gttacaggtg ctgccagagg gaacccggct ggtggattct ggcgcagcga
    39601 ttgctcgccg aacggcctgg ctgttagaac atgaagcccc ggatgcaaaa tctgccgatg
    39661 cgaatattgc cttttgtatg gcaatgacgc cagaagctga acaattattg cccgttttac
    39721 agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt aggctgattt ggttgaatgt
    39781 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    39841 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
    39901 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag cgtattatgc
    39961 acaccccgcg ccgctga
//
LOCUS       DAZGAP010000014        39492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGAP010000014 DAZGAP010000000
VERSION     DAZGAP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.014"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..536)
                     /gene="hemG"
                     /locus_tag="ACHLAM_000669"
     CDS             complement(<1..536)
                     /gene="hemG"
                     /locus_tag="ACHLAM_000669"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO7852251.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPT"
     gene            complement(548..1999)
                     /gene="trkH"
                     /locus_tag="ACHLAM_000670"
     CDS             complement(548..1999)
                     /gene="trkH"
                     /locus_tag="ACHLAM_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO7852252.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2038..2652)
                     /locus_tag="ACHLAM_000671"
     CDS             complement(2038..2652)
                     /locus_tag="ACHLAM_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO7852253.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2652..3983)
                     /gene="pepQ"
                     /locus_tag="ACHLAM_000672"
     CDS             complement(2652..3983)
                     /gene="pepQ"
                     /locus_tag="ACHLAM_000672"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO7852254.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENITRDLKLA"
     gene            4173..6362
                     /gene="fadB"
                     /locus_tag="ACHLAM_000673"
     CDS             4173..6362
                     /gene="fadB"
                     /locus_tag="ACHLAM_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO7852255.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            6372..7535
                     /gene="fadA"
                     /locus_tag="ACHLAM_000674"
     CDS             6372..7535
                     /gene="fadA"
                     /locus_tag="ACHLAM_000674"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO7852256.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMIGDAQTCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLTQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(7727..8428)
                     /gene="fre"
                     /locus_tag="ACHLAM_000675"
     CDS             complement(7727..8428)
                     /gene="fre"
                     /locus_tag="ACHLAM_000675"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO7852257.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8474..9967)
                     /gene="ubiD"
                     /locus_tag="ACHLAM_000676"
     CDS             complement(8474..9967)
                     /gene="ubiD"
                     /locus_tag="ACHLAM_000676"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO7852258.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10134..10622
                     /gene="rfaH"
                     /locus_tag="ACHLAM_000677"
     CDS             10134..10622
                     /gene="rfaH"
                     /locus_tag="ACHLAM_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO7852259.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10619..11401)
                     /gene="tatD"
                     /locus_tag="ACHLAM_000678"
     CDS             complement(10619..11401)
                     /gene="tatD"
                     /locus_tag="ACHLAM_000678"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO7852260.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11443..12219)
                     /gene="tatC"
                     /locus_tag="ACHLAM_000679"
     CDS             complement(11443..12219)
                     /gene="tatC"
                     /locus_tag="ACHLAM_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO7852261.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12222..12737)
                     /gene="tatB"
                     /locus_tag="ACHLAM_000680"
     CDS             complement(12222..12737)
                     /gene="tatB"
                     /locus_tag="ACHLAM_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO7852262.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12741..13010)
                     /gene="tatA"
                     /locus_tag="ACHLAM_000681"
     CDS             complement(12741..13010)
                     /gene="tatA"
                     /locus_tag="ACHLAM_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO7852263.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13089..14729)
                     /gene="ubiB"
                     /locus_tag="ACHLAM_000682"
     CDS             complement(13089..14729)
                     /gene="ubiB"
                     /locus_tag="ACHLAM_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO7852264.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14726..15331)
                     /gene="ubiJ"
                     /locus_tag="ACHLAM_000683"
     CDS             complement(14726..15331)
                     /gene="ubiJ"
                     /locus_tag="ACHLAM_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO7852265.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15345..16100)
                     /gene="ubiE"
                     /locus_tag="ACHLAM_000684"
     CDS             complement(15345..16100)
                     /gene="ubiE"
                     /locus_tag="ACHLAM_000684"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO7852266.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16195..17622)
                     /gene="rmuC"
                     /locus_tag="ACHLAM_000685"
     CDS             complement(16195..17622)
                     /gene="rmuC"
                     /locus_tag="ACHLAM_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO7852267.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17839..18606
                     /locus_tag="ACHLAM_000686"
     CDS             17839..18606
                     /locus_tag="ACHLAM_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO7852268.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            18630..19634
                     /locus_tag="ACHLAM_000687"
     CDS             18630..19634
                     /locus_tag="ACHLAM_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO7852269.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            19624..20253
                     /locus_tag="ACHLAM_000688"
     CDS             19624..20253
                     /locus_tag="ACHLAM_000688"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO7852270.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            20278..21252
                     /locus_tag="ACHLAM_000689"
     CDS             20278..21252
                     /locus_tag="ACHLAM_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFO7852271.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAVAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            21269..21775
                     /locus_tag="ACHLAM_000690"
     CDS             21269..21775
                     /locus_tag="ACHLAM_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO7852272.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            21788..23278
                     /locus_tag="ACHLAM_000691"
     CDS             21788..23278
                     /locus_tag="ACHLAM_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO7852273.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(23312..24076)
                     /gene="udp"
                     /locus_tag="ACHLAM_000692"
     CDS             complement(23312..24076)
                     /gene="udp"
                     /locus_tag="ACHLAM_000692"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO7852274.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            24338..25150
                     /locus_tag="ACHLAM_000693"
     CDS             24338..25150
                     /locus_tag="ACHLAM_000693"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO7852275.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            25542..26675
                     /gene="tssD"
                     /locus_tag="ACHLAM_000694"
     CDS             25542..26675
                     /gene="tssD"
                     /locus_tag="ACHLAM_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO7852276.1"
                     /translation="MSDIIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFI
                     PRQKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFKPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            26672..26929
                     /locus_tag="ACHLAM_000695"
     CDS             26672..26929
                     /locus_tag="ACHLAM_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO7852277.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            27004..27249
                     /locus_tag="ACHLAM_000696"
     CDS             27004..27249
                     /locus_tag="ACHLAM_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO7852278.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(27418..29679)
                     /gene="metE"
                     /locus_tag="ACHLAM_000697"
     CDS             complement(27418..29679)
                     /gene="metE"
                     /locus_tag="ACHLAM_000697"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO7852279.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            29797..30750
                     /gene="metR"
                     /locus_tag="ACHLAM_000698"
     CDS             29797..30750
                     /gene="metR"
                     /locus_tag="ACHLAM_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO7852280.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSCLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(30638..31537)
                     /gene="bioP"
                     /locus_tag="ACHLAM_000699"
     CDS             complement(30638..31537)
                     /gene="bioP"
                     /locus_tag="ACHLAM_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO7852281.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(31613..32413)
                     /gene="yigL"
                     /locus_tag="ACHLAM_000700"
     CDS             complement(31613..32413)
                     /gene="yigL"
                     /locus_tag="ACHLAM_000700"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO7852282.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(32421..33443)
                     /gene="pldB"
                     /locus_tag="ACHLAM_000701"
     CDS             complement(32421..33443)
                     /gene="pldB"
                     /locus_tag="ACHLAM_000701"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO7852283.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVIVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            33554..34174
                     /gene="rhtB"
                     /locus_tag="ACHLAM_000702"
     CDS             33554..34174
                     /gene="rhtB"
                     /locus_tag="ACHLAM_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO7852284.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(34236..34856)
                     /gene="rhtC"
                     /locus_tag="ACHLAM_000703"
     CDS             complement(34236..34856)
                     /gene="rhtC"
                     /locus_tag="ACHLAM_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO7852285.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(34920..36749)
                     /gene="recQ"
                     /locus_tag="ACHLAM_000704"
     CDS             complement(34920..36749)
                     /gene="recQ"
                     /locus_tag="ACHLAM_000704"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO7852286.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPISASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(36881..37750)
                     /gene="pldA"
                     /locus_tag="ACHLAM_000705"
     CDS             complement(36881..37750)
                     /gene="pldA"
                     /locus_tag="ACHLAM_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO7852287.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITRHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            37915..38382
                     /gene="yigI"
                     /locus_tag="ACHLAM_000706"
     CDS             37915..38382
                     /gene="yigI"
                     /locus_tag="ACHLAM_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO7852288.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            38434..39324
                     /gene="rarD"
                     /locus_tag="ACHLAM_000707"
     CDS             38434..39324
                     /gene="rarD"
                     /locus_tag="ACHLAM_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO7852289.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTAK"
BASE COUNT         9620 a        10488 c         9976 g         9408 t
ORIGIN      
        1 gtcggtttgt cggttaaatg ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg
       61 gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg acatcttcat aatcagcttg
      121 atcataaaac ggtcgtacca gcgataacgt gggtaacgca gcgccccggc aatgacggca
      181 cagcgatcgg gacgccattg tgaactcatc agaaacttcc gcgcgtagct gttggtctgt
      241 ggagtacgct tctccggttt acgcgccacc aggtttacgg agtaaaaggc gctcggcatc
      301 gaattcagtc gcgtcgcatg ctttttgaca aactcctgga acgcagagtg gtagtgacca
      361 tagcgaatag aagcaccaat gaccacacgg tcgtagtttt cccactgtgg ttcttcaatg
      421 cggtgcacat tggcgacatc cgcctggatc cccagttctt tcagttccga agccaggtag
      481 gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa gaattaatgt tttcacgtat
      541 tactccatca ttcacgccag aaagtcgggg taaagagcac cagcaatgta aagacctcga
      601 gacgaccaaa cagcatgttg gcaatcagga tccatttagc caccgggttc atactggtaa
      661 agttatcagc aaccacacca agccctggcc ccaggttatt caatgtcgca acaaccgagg
      721 caaaggcaga aaagtcatcc acgcccgtgg cgataatcgc cagcatactg acaataaaca
      781 ccaatgcata ggcggagaaa aatccccaca cggcttcgag gatacgttcc ggcagtgcgc
      841 gattccccag cttaatgcta tagacggcgt tcggatgcac cagtcgtttc agctcacggt
      901 tcccctgctt aaacagcagc aggatgcgga tcactttcag gccaccgccc gttgacccgg
      961 cgcaaccgcc gataaaagct gaacataaga gcagtaccgg caaaaagagc ggccagcggg
     1021 caatgctgtc agtcgtaaac ccggcggttg tcgccatcga taccacctgg aaaaacgcct
     1081 ggttaattgt catcagcgcc gaactgtaga cattatgaaa ccacagtacg agtgtacaaa
     1141 taaccaccag cgtaaactgt acgccgataa acatgcgaaa ttccggatcg cgccaataaa
     1201 ccttcagact acgcccactt aacagtgaaa agtgcagacc gtagttacag ccggagatca
     1261 gcaggaagat agcaatgata gtgttaatag tcgggctgtc gaaataaccg atactggcat
     1321 catgtgtcga gaagccgcca atagcgatag tcgcaaagct atggccgatg gcatcaaagg
     1381 catccattcc ggcaaaccac aacgccagcg cacaggcgac ggtcagcaag acataaatca
     1441 accacagggt tttcgccgtt tccgcaattc gcgggcgcat tttgttatct ttcagcgggc
     1501 cgggcatttc tgcgcgatag agctgcatcc cacccacgcc gaggataggc agtatcgcaa
     1561 ccgctaacac gatgatcccc atcccgccaa accattgcag catctggcga taaaagagga
     1621 tggcgtgagg gagcgaatcc agccccacta gcgtagtggc ccccgtggtg gtcaggccag
     1681 agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg gctttccgag aagataaaag
     1741 ggagcgcacc gacgctaccc agcacggtcc agaacagcac cactatcaga aacccctcac
     1801 gggatttcag ttcgcccttc tctttgcggt tcggccacca cagcatagag ccaatggcga
     1861 gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta
     1921 ccagcccagg gataatcatg gtccctgaaa ataagatgac cagtagtcca acgattcggg
     1981 taatggcgcg aaaatgcatc tctgccgctt ccttagttct gcaaaacgaa gtggggatta
     2041 ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa
     2101 ttcagccact ttcgccgccg gaagcgccac ccgcagcaga acgaatgcct gataatcact
     2161 gttaataatt ttgccgtcac actgccccag caacgcttca atgccggtta actgactata
     2221 ttcacattgc aaagtatatt cggttaatgg tgtcttgcgt tgggtcgtta gctggcgcag
     2281 cgcctgattc acgccgccgc catacgcttt cactaaccca ccggtgccaa gcaatatgcc
     2341 gccgtagtag cgcaccacca cagcggtaat ttccccgacg ccgctgccca ttagttgggc
     2401 gagcatcggt ttacctgccg ttcccgccgg ctccccgtcg tcagagaaac ccagctgttg
     2461 agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg tggcgggcat cggggtgctc
     2521 tgcccgcacc gattcaacaa acgcttttgc cgcctcaacg ccatcggtat gcgccaacag
     2581 cgtaatgaag cggctctttt tgatctcttc aacgaccgtg accggtgccg caggaattaa
     2641 ccagctttcc atcacgccag tttcagatcc cgggtaatgt tttccacgtt gttttcgtga
     2701 atcaccacgt tgtcttcgat acgaatgccg ccgaacggtt tcagtgcttc aattttctgc
     2761 cagttgaagt gtttgctgaa ctgcccttca cgccacggtg ccagaagcga ttcgatgaag
     2821 tagatacccg gttcgatggt taacaccatg cccggctgga gaatacgggt gcagcgcagg
     2881 tacggatatt ttgccggtgc cgcgaggtgc gtaccgctat catcctgcat aaaaccggct
     2941 acgtcatgca cctgcaggcc cagcggatgg ccgataccgt gcggcataaa cggtccggtg
     3001 agatcgtttt cgaccatcgc ttcttcactc atatcggtga tgatttgatg tttacgcagc
     3061 agtttggcga tgcgctgatg gaactggata tggtaatcca catagctgac gcctgctttc
     3121 atggtagcga tcagcgccag ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg
     3181 ttgtcgcttt ttgccgacca ggtacgggtt agatcagccg catagccgtt atattcggcc
     3241 ccggcatcca gcaggaagct gcgcatctct tccggtgcct ggtggtccag tttggtgtaa
     3301 tgcaacaccg cagcgtgttc gttaagcgcc acaatgttgc tataaggtac gtcggtatca
     3361 cgatgaccgg tcgcggtcag ataggcaatg ttgatatcga actcgctcat gccagaacgg
     3421 aacgcttctt ctgccgcgcg atgaccgttg accgccattt tctgcgcttc acgcatacag
     3481 gccagttcgt actcggtttt gaaggagcga tagtaatgca ggtagtcgat cacccctttc
     3541 gggttgatat tgctggcctc aatacccagt tgcagcgcac gttctggcac cggaccgata
     3601 taaccgatat tgccgcgcgc agcaggcaac agactaccaa tgccatcggc tttcggcagc
     3661 gcgatcactt ctacatcttc agtccagaag gagttcggca gcggttcgac gttgtgccag
     3721 taatcaaccg gtagatagaa ccacagtttc ggcttattca cgccatccac cagcagccag
     3781 cagtttggca cctgagttac tggcacccac gctttgaatt gcgggttcac tttaaacgga
     3841 tagggatgat cgtcgagaaa aacgttgaac agttcgccgg agtgaataag taacgcatcc
     3901 agcttgaagc gcgccagcgc atcgcgagtc cgttcctgta aggtagctat atgattttta
     3961 tagagcgagg ccagtgattc cattttttac ccttctgttt ttttgacctt aagtctccgc
     4021 atcttagcac atcgttcatc cagagcgtga tttctgccga gcgtgatcag atcggcattt
     4081 ctttaatctt ttatttgcat atttttaaca caaaatacac acttcgatgc atctggtacg
     4141 accagatcac cttgcggatt caggagactg acatgcttta caaaggcgac accctgtacc
     4201 ttgactggct ggaagatggc attgccgaac tggtatttga tgccccaggt tcagttaata
     4261 aactcgacac tgccaccgtc gccagcctcg gcgaggccat cggcgtgctg gaacagcaat
     4321 cagatctaaa agggctgctg ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata
     4381 tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca gttaagtcag tggctgcact
     4441 ttgccaatag cgtgtttaat cgcctggaag atctcccggt gccgaccatt gctgccgtca
     4501 acggctatgc gctgggcggt ggctgcgaat gcgtgctggc gaccgattat cgtctggcga
     4561 cgccggatct gcgcatcggt ctgccggaaa ccaaactggg catcatgccg ggctttggcg
     4621 gttctgtacg tatgccacgt atgctgggcg ctgacagtgc gctggaaatc attgccgccg
     4681 gtaaagatgt cggcgcggat caggcgctga aaatcggtct ggtggatggt gtagtcaaag
     4741 cagaaaaact ggttgaaggg gcgatggcga ttttacgcca ggccattaac ggcgacctcg
     4801 actggaaagc gaaacgtcag ccgaagctgg aaccgctaaa actgagcaag attgaagcca
     4861 ccatgagttt caccatcgct aaagggatgg tcgcacaaac ggcggggaaa cattatccgg
     4921 cccccatcac cgcagtaaaa accattgaag cggcggcccg ttttggtcgt gaagaagcat
     4981 taaatctgga aaacaaaagt tttgtcccgc tggcgcatac caacgaagct cgcgcactgg
     5041 tcggcatttt ccttaacgat caatatgtaa aaggcaaagc gaagaaactc accaaagacg
     5101 ttgaaacccc gaaacaggcc gctgtgctgg gcgcaggcat tatgggtggc ggcatcgctt
     5161 accagtctgc gtggaaaggc gtaccggttg tcatgaaaga tatcaacgac aagtcgttaa
     5221 ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg
     5281 atggtctgaa actggctggc gtgatctcca caatccaccc aacgctcgat tactctggtt
     5341 ttgaccgcgt ggatgttgtg gtagaagcgg ttgtcgaaaa cccgaaagtg aaaaaagccg
     5401 tactggcaga aaccgagcag aaagtacgcc cggataccgt gctggcgtct aacacttcaa
     5461 ccattcctat cagcgaactg gccaacgcgc tggaacgtcc ggaaaacttc tgcgggatgc
     5521 acttctttaa cccggtgcac cggatgccgt tggtggaaat tattcgcggc gagaaaagct
     5581 ccgacgaaac catcgcgaaa gtcgtcgcat gggcgagcaa gatgggcaag acgccgatcg
     5641 tggttaacga ctgccccggc ttcttcgtca accgtgtact gttcccctat ttcgccggtt
     5701 tcagccagtt gctgcgcgac ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac
     5761 agtttggctg gccgatgggt ccggcatatc tgctggatgt tgtgggcatt gacaccgcgc
     5821 atcacgccca ggctgtcatg gcagcaggct tcccgcagcg gatgcagaaa gattatcgcg
     5881 atgccatcga cgcgctgttt aatgccaacc gctttggtca gaagaacggc ctcggtttct
     5941 ggcgttataa agaagacagc aaaggtaagc cgaagaaaga agaagatgcc gtcgttgacg
     6001 acctgctggc agaagtgagc cagccgaagc gcgatttcag cgaagaagag attatcgccc
     6061 gcatgatgat cccgatggtc aacgaagtgg tgcgctgtct ggaagaaggc attatcgcca
     6121 ctccggcaga agcggatatg gcgctggtct acggcctggg cttccctccg tttcacggcg
     6181 gcgcgttccg ctggctggac accctcggta gcgcaaaata cctcgatatg gcacagcaat
     6241 atcagcatct cggcccgctg tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata
     6301 acgaaccgta ctatcccccg gttgagccag cccgtccggt tggcgacctg aaaatggctt
     6361 aaggagtcac aatggaacag gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt
     6421 cgaagggcgg tgcttttcgt cacgtgcgtg cggaagatct ctccgctcat ttaatgcgta
     6481 gcctgctggc gcgtaacccg gcgctggaag cggcggccct cgacgatatt tactggggtt
     6541 gtgtacagca gacgctggag cagggtttta atatcgcccg taacgcggcg ctgttggcag
     6601 aagtgccgca ctctgtcccg gcggttactg tcaatcgctt gtgtggttca tccatgcagg
     6661 cactgcatga cgcagcacga atgatcatga tcggcgatgc gcagacatgt ctggttggcg
     6721 gcgtggagca tatgggccat gtgccaatga gtcacggcgt cgattttcac cccggcctta
     6781 gccgtaatgt cgccaaagcg gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta
     6841 tgcacggtat cagccgtgaa atgcaggatg cctttgccgc gcggtcacac gcccgcgcct
     6901 gggccgccac gcagtcgggt gcatttaaaa atgaaattat cccgaccggt ggtcacgatg
     6961 ccgacggcgt cctgacgcag tttaattacg acgaagtgat tcgcccggaa accaccgtgg
     7021 aagccctcgc cacgctgcgt ccggcgtttg atcccgtcag cggtacggta acggcgggta
     7081 catcttctgc gctttccgat ggcgcagctg ccatgctggt gatgagtgaa agccgcgccc
     7141 atgaattagg tcttaagccg cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc
     7201 cgtcgattat gggttacggt ccggttccgg cctcaaaatt ggcgctgaaa aaagcggggc
     7261 tttctgtcag tgatatcggc gtgtttgaaa tgaatgaagc ctttgccgcg cagatcctgc
     7321 catgtattaa agatctggga ctgattgagc agattgacga gaagatcaac ctcaacggtg
     7381 gcgcgatcgc gctgggtcat ccgttgggct gttccggtgc gcgtatcagc agcacgctgc
     7441 taaatctcat ggagcgcaag gatgcccagt ttggtcttgc gacgatgtgt atcggtctgg
     7501 gtcagggtat tgcgacggtg tttgagcgga tttaaccact tgccagatgc ggcgtgaacg
     7561 ccttatccgg tctaccgatc ctgcaccaat tgtaggcctg ataagacgcg ccagcgtcgc
     7621 atcaggcagc gtgtgccgaa tgccggatgc gacatagatc taaatgccac tgtataaagt
     7681 tttagttgcc gttcttcccg cctgtcaggg gcgggttttt tatatctcag ataaatgcaa
     7741 acgcatcgcc aaacaggcga tcttcccgcg cattacgctc gctgcaaaac aggtcacggg
     7801 caattttcgc catctcaaaa cgtccggcaa tatagatatc atgctctgcc agcgtaccat
     7861 gatcctgcaa taccgccgtt aacacggtgc cagtacgccc acgccagccc gcttccggtt
     7921 gttcaaccac cggcaccact tgtagaccag gatgcttcaa cgaaagcgcc tcaagctcgc
     7981 agagatcata cagatgctgc tcttcacgcc cgccccagta aatggtgata tcacggtttg
     8041 ggttacgcgc caacgctgtc agcaaaatcg agcgggcata agagaacccg gtgccgcccg
     8101 caatcaaaat catcggacgc tcttcatcat cgcgcagcca cgcttctccg tggggaatat
     8161 cgaccacgat ttgatgatct ttgaggatgc ggtccatgac tgctttcgcg taaaggttga
     8221 tttcagaagc gccaatatgc agctcgataa accctttttc atctggcgtc gaagccattg
     8281 agaacggacg tttgtcgcgc tcatccatca ctaccatcaa atactgacca gcacgaaaag
     8341 aaaaggccgc gtctggcacg atgcggacac gatatacggt atccgtgata gcttctaccg
     8401 aggtcacttt acagcttaag gttgtcatgc gctttctctg tcggatcgat aaatagggca
     8461 aaacaaacgc gcatcaggcg cttttaccgt tgttaaaaat agccagttca tcccaaatgg
     8521 cgtcaatatg cgcgacaaca tctggatctt ttttgatggg acgtccccat tcacgctggg
     8581 tttcccccgg ccatttattc gtggcatcca gccccatttt tgaacccagc ccggagacag
     8641 gggaggcaaa atccagataa tcaataggcg tattttctac cagaacggta tcccgcgccg
     8701 ggtccatacg ggtggtaatc gcccaaatca catcgttcca gtcgcgggcg ttgacgtcat
     8761 catcgcaaac gatcacaaat ttagtgtaca taaactggcg taagaacgac cagacgccca
     8821 tcatgacgcg cttcgcgtgt ccggcatact gttttttgat cgtcactacc gccagacgat
     8881 aagaacagcc ttccggcggc agataaaaat cgacaatttc cgggaactgt ttttgcagaa
     8941 tcggcacgaa cacttcgttc agtgctacac ccagtaccgc aggttcatct ggcggacgcc
     9001 cggtataggt ggaatgataa atcgcatctt cacgctgggt aatatgcgtc acggtaaata
     9061 ccggaaaact atcgacttcg ttatagtaac cggtgtggtc gccatacggc ccttccggcg
     9121 cagtttcgcc ttgttcgata tacccttcca gcacaatctc cgcactggcg ggcacttcaa
     9181 ggtcattgga gatacacttc accacttcgg tcttggtgcc gcgcagcaat ccggcaaacg
     9241 catactctga aagcgtatcc ggaacggggg tgactgcacc gagaatcgtg gcgggatcgg
     9301 cacccagcgc cacagaaacc gggaaacgtt cgcccggatg cgccgcacac cactcctgat
     9361 aatccaacgc gccgccgcga tgcgacagcc agcgcataat cagtttgttt ttaccaatta
     9421 gctgctggcg ataaatgccc agattctgcc gctctttatg cgggccgcgc gttacggtga
     9481 gcccccaggt aatcagcggc gcggcatctt ccggccagca ggtcataatg ggaatacgat
     9541 tgagatcgac gtcatcgcca gagacgattt tttgttggca aggcgcacca cgcagccgct
     9601 ttgtcggcat gttcaatact tgcttaaact gcggcagttt atcaaacagg tcgcggaaac
     9661 cttttggcgg ctccggctct ttcagaaacg ccaataattt accaacttca cgcagcgccg
     9721 aaacatcttc ctgccccatg cccatcgcca cgcgctttgg cgtaccgaac aggttgcaca
     9781 gcaccggcat tgagtagcct ttagggtttt cgaacaacag cgcaggccca ccggcacgca
     9841 aagtgcggtc agcaatttca gtgatttcca ggtgcggatc caccgggagc gtgatacgtt
     9901 ttagctcacc ctgctgttca agcagcgtca agaagtcgcg taaatcgtta tatttcatgg
     9961 cgtccattgt agcctcttaa tctgcgccca ttatacggcg ttcatctttg cgatgctgta
    10021 aatttgttaa attagcgtga actctgacgg tataacgcaa accggggaat ataattaact
    10081 tagcgtaaag cttttgctat ccttgcgccc cgattaaacg gataagagtc attatgcaat
    10141 cctggtattt actgtactgc aagcgcgggc aacttcaacg tgcccaggaa cacctcgaaa
    10201 gacaggctgt gaattgcctg gcaccgatga tcaccctgga aaaaatcgtg cgtggcaaac
    10261 gtactgcagt cagtgagcca ttgttcccca actacttgtt tgtggagttc gatccggaag
    10321 tgattcatac cacgactatc aacgcgaccc gcggtgtcag ccacttcgtg cgctttggcg
    10381 cgtcgccagc gatagtccca tcggcggtga ttcatcagtt atcagtgtat aaaccgaaag
    10441 atattgtcga tccatcgacg ccgtatcctg gtgataaagt gattattact gaaggcgcat
    10501 tcgaaggttt tcaggccatt ttcaccgagc cagacggcga ggctcgctcc atgctattgc
    10561 tgaatcttat taataaagag attaagcaca gtgtgaagaa caccgagttc cgcaaactct
    10621 aaaacgcaat cccaaacagt gttttgacat tagcatccgt ggtggcagcc agccatgcgg
    10681 catcttctcc acgccagtgc gcaatacgtt gcaaaatatg gggcagatgg gctggctcgt
    10741 tgcgccggga tgatggcttt ggcgtgagat cgcgagggag cagatacggc gcatcagttt
    10801 cgatcagcaa tttctccgcc ggaatcaacg gcaacaattc ccgcagctcc agcccgcgtc
    10861 gttcatcgca aacccaaccg gtaatgccga tataaattcc atgcgccacg cacgcctgca
    10921 tctcttcgcg tgtgccggta aagcaatgaa gaaccgcacc aggcacttta tccagccacg
    10981 gctccagcaa tgtcataaac cgctcgtggg catcgcgaca gtgcataaat accggcatgt
    11041 ttaattctgc ggcaatgcgt agctgggcaa caaaagcgcg ttcctgttct tccggcgtcg
    11101 aaaagttgcg gttaaagtcg agaccacatt caccaatcgc caccacttct ggctgcgcgg
    11161 ccagctcaat aatcgcttct tcagtcgcag cttgccactg gctgctgtcg tgaggatgta
    11221 cgcccgccgt tgaccaacag gacgaatact gacgcgccag cttttgcgcc tgctggcttt
    11281 cacgcaggtt agtgccggtg atgagtagcc cattaactcc cgcgtcaaaa gcgcgcgcta
    11341 caacatcatc acggtctttc gcaaattgcg aactggtcaa attaacgccg atatcaaaca
    11401 tcctgtactc catatgacaa ccgccctgac gggcggttga atttattctt cagttttttc
    11461 gctttctgct tcagcgtcgt tttcctcttc ccgatttcgc cctttaccaa cgtaaaagcg
    11521 tgagaagaag acaccgattt caaacaggca atacatcggg atcgccagca gcgtttgcga
    11581 gaagacatcc ggcggcgtca gcagcatccc gacaacaaat gcgccaacca gcacatacgg
    11641 acgttttttg cgtaagtctt ctggcgaggt aattcccatc cagcacagca gcactattgc
    11701 caccggcact tcaaaggaga caccaaacgc cataaacagc gccataacga agcttaaata
    11761 gctggcgatg tcggtggata cctgtacccc ttccggcgcg gtattggcaa ggaagccaaa
    11821 tgccagcgga aagaccacaa agtaggcgaa cgccatgccg atataaaaca acagagagct
    11881 ggaaaccagc agcggcacca ccaggcgacg ttcatgctta tacagcgctg gggcgataaa
    11941 cgcccacacc tgatagagaa tcaccggcgc tgacagaatc agcgacacca taaaggtcag
    12001 cttgatcggc gtaaagaacg gcgaggccac gtcggtggcg atcatcgttg aaccttgcgg
    12061 caactgcttg atcaatggcg cggataccag gtgatagatg tcattggcga aatagaccag
    12121 acacaggaat atcacgatca ccgcgataat gcagttcagc agacgcttac gcagctcaat
    12181 cagatgcgtg ataagcggtt gagtatcttc tacagacatg tttacggttt atcactcgac
    12241 gaaggggaag gtgcagcggt tttcggttca gcgtccgcag caggttttac caccggctct
    12301 ggcgtggttt ctggcttctg ttccggcgaa ctggcttgcg tttgtgcagc agcaggcgtt
    12361 acgccttcat gcgcagcttc attgtctttc accaccgggt tgtggatggt gtgcgcttca
    12421 tcgctcgcct tttcaggatc gtttgcaacg taggaacgct tcatcgactc cgcggcctgg
    12481 cgtagttcat ccatcgacgc tttcagttcg ggcgtcaggt tagtgaggct cgccttttca
    12541 acctttttca gactgtcctg aaactcctgg agttttaact cctgggtcag ttcgttctgc
    12601 accgttgtcg ccagtgaacg caacgcgcga atccagcccg cgaccgtttt taccgccaca
    12661 ggcagtcgtt gcggccccag aacgacgagg ccgatgatga ataccaatag cagttcgcta
    12721 aaaccgatat caaacacgga ttacacctgc tctttatcgt ggcgcttcgc gtcttctgtt
    12781 ttagcctgtt cctgattcgt atccgcctgc ttatcggcga tagttttcgc agtaaaatca
    12841 gcatcctgac tggttttatc ctgctttggt tcatcatcgc tcattgcttt tttaaagcct
    12901 ttgatcgacg caccaagatc ggaaccgatg gagccgagct ttttggtgcc aaaaagcagt
    12961 acaacgatga cggcaataat caataactgc caaatactga taccacccat acatgttcct
    13021 ctgtggtaga tgatgattaa acaaggccgc attatacgtt acacggcccg ccttgagcga
    13081 tgaaaaaatc agcgtgtttt gcgccaaccg acaaaccagg cgatcagacc acctgccatt
    13141 aaccagccgg gcatcagccc ccattcaggt cggctgacca acaagaatgt gccacttaat
    13201 actaacgtag cgccaattcc gagaaaataa cgcgattgtc cctgacgtac atgatttgac
    13261 tgaagctcgc gggcaatctt atcaacactg tgctgtaaat acttgccctg gcgcaaactg
    13321 tcgtaaacca gttcaggcag ttctggcatt ttttcgaccc agaacggcgc tttttcttta
    13381 aatgctctca ccagcgcagg aataccgacc tgatctttaa tccacgactc aaggaaaggc
    13441 ttcgccgttt tccataaatc gagctgcgga taaagctggc gtcctacccc ttcgacgtag
    13501 agcagggttt tctggagtaa caccagttgc ggctgcactt ccatattgaa gcgacgcgcc
    13561 gtattaaata gatttaacag cacatgtcca aacgaaattt cggccaacgg tttctcaaag
    13621 ataggttcac agaccgtacg aatggcaaat tcgaactctt caacgttggt atctggcggc
    13681 acccagcctg aatcgacgtg tagctctgca actttgcgat aatcgcgatt aaagaaagcg
    13741 ataaagtttt ccgccagata gcgtttatct tctttgttta gcgagccaac aatcccgcaa
    13801 tcaatgccga tatatttcgg gttttctggg tgttcatagc ttacaaagat gttgccaggg
    13861 tgcatatcgg catggaaaaa gctgtcgcga aagacctgag tgaagaacac ctgcacgccg
    13921 cgttccgcca gcaatttcat gttggtgccg tttttctcca gcgtcgcaac atcagacacc
    13981 ggaatgccgt aaatacgctc catcaccatc atcccttcac tacaatagtc agggtaaact
    14041 tccgggatgt agagcatcgg gctgtcttca aaattgcgtc gaagctgaat ggcgttggca
    14101 gattcccgca gcaaattcag ttcatcaatc aaggtctttt cgtactcgcg caccacttcg
    14161 gttgggcgca gacggcgacc atccggcagc aaacgcggca cccagcgagc cagacggtag
    14221 ataagtttca gatccgcttt aatgaccggc aaaatatccg ggcggatgac tttaatcacc
    14281 acctctttac cattcgattt caatcgcgcg gtatgaacct gggcgataga agcagaagcc
    14341 agcggcttga tttcaaaatc gtcaaaccac gcttctaccg gcaagccgcc cattgcagct
    14401 tcaatctgct gcttcgccag cttgccatca aacggagcga ctttgtcctg caataacgcc
    14461 agctgatcgg caatatgcgg cggaaaaaga tcgcggcggg ttgataacat ctgcccgaac
    14521 ttgatccata ccggtcccag ttcttgcagg gccagtcgta gtcgctcacc taaaggtttg
    14581 tctttatgcc gatttggcat ccagaataat gagtatcgcc atagccgtag cggcagggtg
    14641 atacgcattt tggggatcag ttcatcaagt ccgtagctta aaaaagtgcg aatgatgaaa
    14701 tataggcgcc gtacttcacc tggcgtcatt tagcctccag tttttccagc cgtttggtca
    14761 gggcatcaac agcacgctcg acggcagccg tctcttccgc aaaccaggcc acttcaagcg
    14821 gaccgggtgc catacgccac tcttcagtaa tggcttccgc cacataacgt tgctggcgct
    14881 taatgccgtg atgcaggaac tttgcgcccc cgcgcagggc tttgctgata ccttcagcgg
    14941 cgatatcacc ggtataaggg gccagcagtt ccgcagggtc gaactcagcc agatctgcca
    15001 gcgcaacgaa gttttgcacc acctgaatat cgccctgcac ttccagctca ccactgcgaa
    15061 tcagtgcggc aagctgctgg cgatcgcgaa gtttcggcaa cacactggcg taggcgataa
    15121 cggtgcagtc agcatcgcct gcccattcgc ccagtacatc aacctggcgt tcgctgaaca
    15181 ccagaatcaa tgacgtcgaa aagcctttta cctccacgcg cagcactttg cccaacagac
    15241 gcgagcgggc cgttttcagc gcgggtgagc gatacaggaa ggtgttgagc agactttcaa
    15301 ttcctgccgt cactaaaggt ttaaaaggca tttccggtct cctgtcagaa cttataacca
    15361 cgatgcagcg ccacaacccc tgccgtcaga ttgtagtagt cgacactttc gaatccggca
    15421 tcctgcatca tggttttcag ggtatcctga tcgggatgca tacggatgga ttctgccaga
    15481 taacggtagc tgtcggcgtc gttcgcgacc agtgagccaa tacgcggcag cacatggaag
    15541 gagtatgcat cataggcttt gctcagcggc tcgataattg gcttcgagaa ctcaagcacc
    15601 agcaggcggc cgccgggttt cagcacgcga tacattgaac gcagtgcttt atctttgtcg
    15661 gtgacgttac gcagaccaaa cgaaatggtg atgcaatcaa aggtgttatc cgggaacggc
    15721 agcgcctcag cgttcgcctg aacatactca acgttgccaa tcacaccgat attacgcagc
    15781 ttctcgcggc ccattttgag catggattca ttgatatcag caaggaccac tttgccagtt
    15841 tctccgacca ggcgagagaa tttcgctgtc aggtcgccgg tgccaccagc cagatccagc
    15901 acggtctgcc cacggcgtac gccgctgcaa tcaatcgtga atcgcttcca caaacgatga
    15961 ataccgaatg acatcaaatc attcatgaca tcgtattttg atgccacgga atggaaaacg
    16021 tgggcgacca tatccgcttt ttgttccttc gcgacggtct gaaaaccaaa gtgcgtcgtt
    16081 tcttgtgact tatccaccat ctcaatgcct gctcatcaaa aaattgttcc agaagtgtaa
    16141 cagattgggc gtcgatgccc tagatttcta cccggcttaa ctacccccaa tgggctagcg
    16201 cgactgctga ttatattcat catcgcgttg ataagcttca tcattcggct gctccggaac
    16261 cgaccgaagt cgatactctt catcctggct caccgcctgt tcagccaaat ccggattaat
    16321 ctcgcgttta atttctaccc ctaaaccgcg aaacgcttct gcctgcgcca gcacatttcc
    16381 gcgacctgaa gagagttttt tcattgcctg gcggtagtta tcctgcgctt tgtcgagact
    16441 ttgaccaatc gctgacatgt catcgacgaa caaacgcatc ttgtcgtaca gcttgctggc
    16501 acgatcggcg atttgctggg cgttgcggct ttgatgctca taacgccaca ggttggcgat
    16561 agtgcgcagc gccaccagca gcgtagtcgg gctaaccagc atgatattgt ttttcaacgc
    16621 ttcggtgatc agctccggct ggcggtcaag cgccagtaaa aaagcgggtt caacgggaat
    16681 aaacatcagc acgtaatcca gggttcgcag ccccggcagc tgttgataat ctttgcgtcc
    16741 cagcaaacgg atatggttac gcaccgacgc gatatgttcc tgtagcgcgc tttcgcgggt
    16801 gtagtcatct tcggcgttga aatagcgttc ataggcgacc agcgtcattt tggcgtcgat
    16861 caccacatct tttccctgcg gcaaccgcac gatgacatcc ggctgcatcc gcgagcgggc
    16921 gtcattttcg atgctgacct gagtttcata ttcataccct tcacgcagac cggaagcctc
    16981 cagcacccgc gtcaatacca cctcgcccca gttgccctgg gttttattgt cgcctttcag
    17041 cgcgcgcgtc aggttgatcg cctcctgagc catttgcgcg ttgagctgct ggagattgcg
    17101 aatttcgtgg gtcagggtat gacgttcctg tgcttcttta ccgaagctgt cctgaacctg
    17161 acggcggaaa ccgtccagtt gttcacgtag cggcgacaac aggctgttca gactctgacg
    17221 gttttgctca tcaacccggc gattgctgtg ctcaaaaata cggttggcga ggttttcaaa
    17281 ctgctcactg aggcgctgct cgctgttgat catctggcga attttatcgt cagcatgttg
    17341 ctgtgcggct tccatccgcg tggttacttc acgcagatcg gcctccagag aggtgttaat
    17401 actttgcagg ctgcgcactt cgttattgag taactcgcac tcagcgcgcc agtgctcgct
    17461 ttgagtaatt tgttgttttg ccgcgcttaa ctccgcgacc atctcttcac gttcagctaa
    17521 ttgctcggct ttttgctgcg catgttgata actggcaaac agccagccaa tgaccacact
    17581 aaccagcgca ataatggcat aaacaatgat tgagaaatcc acaaagcctc ctgacccaac
    17641 aacttacccg cacaaaatag aattgtgctg tacaaacgtc cagtttgaaa gaagtttcct
    17701 gcgaggcgct acgcaaaacg atcgttaatt ttcattattt tatcccacaa tatgagatgg
    17761 agtttatatt gtgggatata tcaccttttc cccacgtcat tatgtggaac acttcagcaa
    17821 tatcatcagg agaaacgtat ggcagaaacc tcttcaacca ccgcaggcgc acaaacgtta
    17881 ttacgtggac tggcggttct taatgctgtt tataacggct gccatgacct aaaaagtatc
    17941 ggcgagttca ccggcacgac ccggagcaca acccatcgtt tagtcaccgt tctggtggaa
    18001 caacgctacc tgcgccatgt cccaacgcag ggctaccaac tgggtgccaa actgatcgaa
    18061 ttcggtgcca gagcactgga aagcacctca ctgtacgaaa ttgcccagcc cgttttgcaa
    18121 cgccttgccc gttacaccct ggacaccgtg catctgggga tcgttgaagg cgacgaagtg
    18181 ctgtatctgg agaagatcaa cagtcagcgc gggctggaaa tgcgctcacg cccaggccat
    18241 cgcatgccac tggcaatcac cggcatcggc aaggcattga tcctcaaccg aacagaagaa
    18301 gagtggcgca cgctgtttaa gacctgtggt gatgaaacaa aactgggcgc atttattcag
    18361 aacatgcgtc gttatgccgc cagtggtttt gcttttgatc tggaagagaa cgagccgact
    18421 atccgttgcg ttgcagcgcc ggtttacaac gcccgggatg aaattgtggc ggctatttct
    18481 gtcgccagta ccaccacgta tatgtcgctg gcgcgtctgg aagaactggc accttacgtc
    18541 aaatcctgtg cagaagagat atccgctgaa ctgggctggg gtaagcacgt ccgcaaagat
    18601 aaataaacca tgattaaagg aaacgactaa tgccgggagc accctcattt attgccctgg
    18661 actggggaac gtcatcgctt cgggcatggc gttttggcga atcaccaaat ccacaagaaa
    18721 aacgtgaatt cccctggggg atcatgaaat taccctccca ggcagcaaca cgtgaggacg
    18781 ctttccacga tacgttttta cgtgtctgcg gcgactggct ggcacaaacc ccatgcccgg
    18841 tactggcttg cggcatgctc ggcagtgcgc aagggtggca atcagcagct tatttacctt
    18901 gcccggtcac tctcgagggt ctggcgaagc agttgacgcc tgttatccac cagcagcaga
    18961 caatgctgca catcattcct ggcgtgatta aagaaggtga aatgcccgaa gtgatgcgtg
    19021 gcgaagagac acaaatcttc ggtgctatct cgatggaacc ggccctgcaa aacgcgatcc
    19081 atcagggtat gccagtgctg ataggcttac ccggcacaca tgcgaaatgg gcagtagttg
    19141 aaaacaacac cattaccgat ttccgaacct ttatgacggg cgagttattt gatgttttat
    19201 cccgccattc gattctcggt gccaccatgc atcctggaga tgaaccgcac tgggatgcct
    19261 tcacccacgg gctgacagca gcacaagagc atcatcagac cggattatta tcgacgctgt
    19321 tttcgacccg ttcgcgcctg ctgaccagta atcttacgtc gtcctcgcag ggggattacc
    19381 tttccggatt actgataggc catgaattat gtggtctggc atccagtttg ctacgtgatt
    19441 tacccgccac aacaccgatc gccttaatcg gtagcgcaaa tctgaacagc cgctattcac
    19501 aagcattcag ccacgttttt cccgacagac agatacacgc cattcctaat gccactgaac
    19561 atggattatg gcgaatcgcc cacgctgccg ggttactgtc caccaacgcc agggaatgta
    19621 cccatgccat ttaatacact tttgcaaaaa accgggttag tcgccatttt acgcggcgtg
    19681 aaaccggatg agattgtcgc catcggtgaa aaactgtacg ccgccggatt ccgcctgatt
    19741 gagattccta tgaactcgcc ggaggcgttg caaagtatca gtttactgcg cgacgccctg
    19801 cctaaggact gcctggtcgg agccggaact gtgctttcgg tagagcaggt ggttgcggtt
    19861 aaagaggctg ggggccagat tatcgtgatg ccgcattgcg atactgcggt gatccgtcgc
    19921 gcccgtgcgc tgggtatgta ttgtgcgcct ggcgtcgcca cgcccaccga agcctttgct
    19981 gccattgaac atggtgctaa tgccatcaag ctgttcccgg ctgaacaaat tacgccagaa
    20041 gttaccaaag cctggcgtgc ggttattcca caatccgttc cgatgttacc ggttggcggt
    20101 atcacaccgg aaaccatggc gcgttatctg agccacggcg caaacggctt tggcctcggt
    20161 tctgcgctat atcgccccgg catgacgcca gagcaagtct atgaaaatgc agtactgttt
    20221 atgaacgcat ggaacaattt aaatagcaac taactgataa ataaggatat aaaagcaatg
    20281 ataaaacatc ttaccttacc gaaaaaaatg gctgcaatgg tacttcttgc aggcgctgcc
    20341 ctcagcattg cgcctgtaca ggctgcctcc tacccaacca aacagatcga gttagtcgtt
    20401 ccctacgctg ccggaggcgg tacggatctg gttgcccgtg cctttgctga tgccgccaaa
    20461 aaccatttac ccgtcagcat cggggttatc aataaacctg gcggaggcgg tgctatcggc
    20521 ctgagtgaaa tcgccgctgc ccgccctaac ggttacaaaa ttggtttagg cacggttgaa
    20581 ctgaccaccc ttcccagcct cggaatggtg cgttttaaaa ccagcgactt taaacccatt
    20641 gcccgtctga atgcggatcc ggctgctatc acagtccgtg ccgatgcgcc gtggaatagc
    20701 tatgaagaat ttatggctta ctccaaagcg aatcccggaa aagtacgcat tggtaactca
    20761 ggcaccggag ctatctggca tctggcggta gctgcactgg aagacaaaac gggcacaaag
    20821 ttttctcatg tcccgtatga cggcgcagcc cctgccatta caggcctgtt aggcgggcat
    20881 attgaagcgg tttccgtaag cccaggagaa gttatcaacc atgtgaatgg cggcaagctg
    20941 aagacactgg tagtgatggc ggatgagcga atgaaaacca tgcctgacgt cccgacgtta
    21001 aaagagaaag gcgttgatct ctccatcggc acctggcgcg gcctgattgt gtcgcaaaaa
    21061 acgccgcagg atgtggtgga tgttctggca aaggcagcaa aagagacggc tgaagagcct
    21121 gcattccagg atgcactgca aaagttgaat ctcaactatg catggcttga cgctgccagc
    21181 ttccagaccc aaatcagcga acaggaaaag tactttgacg agttgctgac tcgcctgggc
    21241 ctgaaaaaat aaacctgagg tacggttgat gaacacaaaa cagtctgttg ctcaactcgc
    21301 cgtaccgcac cgcaagcgcc tttcatcaac gatggtggtg gcgctgttac tttgtgtggt
    21361 tgctggcgcg gtgatgatta atgccgctga ttttccagca actgccattg aaacggatcc
    21421 cggtgcaagt gccttcccta ccttctatgc ctgtgccctg attgtgctcg ctgtcttgct
    21481 ggtgatacgc gatcttttgc aggcaaaacc agcctcttgc gccaacgcac aggaaaaacc
    21541 ggcattcagg aaaacagcaa caggaattgc ggcaaccgcg ttttatattg tggcgatgag
    21601 ctactgcggt tatctcatta ctactcctgt tttcctcatc gtcattatga cgttgatggg
    21661 ctacaggcga tgggtactca caccgggtat tgcgctgctg ttaacggcaa tcctctggtt
    21721 gctgtttgtc gaagcgttac aggtgccatt gcctgtcggc acatttttcg aataacaggg
    21781 gccagcaatg tttgaaagtg aacttttaac tcagggtttt tcgacattac tcaataatcc
    21841 tcaggcgctg ctgtttgcca cttttggggt gatgctgggt attgtgattg gcgctttgcc
    21901 tggtctgaca gcgaccatgg gtgtggcgat tttgctgcct ttcacctacg gcatggagcc
    21961 tgtttctggc ttgttgatga tttgcggcgt cttttttggt ggcgtctacg gtggttctat
    22021 caccgcaatt ttgctcaaaa ttcccggaac gcctgccgcc gcagccaccg ctattgatgg
    22081 ttatgagctg acgaaacagg gaaaagcagg gctggcattg agtgccgcca cgttctcttc
    22141 ctttagtggc ggaacgctca gcattatcgt gctgatgttt ctctctccgg tacttgccag
    22201 ttgggcgctg aaatttagtg cctcggagtc cttcgccctg gcaaccttcg gactgagtat
    22261 tattgccagt atttccggtg agtcgctgat taaagggctg attgccgggg ttggcggatt
    22321 gctgatcgca acgataggcc ttgatccaat gggcggtttt ccacggttta caggtggatt
    22381 tgtggagctg atgaatgtgc catttatccc ggtgatgatt ggtttatttg ctgcttcaga
    22441 agcattccgc tctatggagc aaaaccagca aattcgtcag ggggcgaagg tggctatcgg
    22501 cagtctgtta ctgccctggc aaacactacg ccgcattgca ttaaccattt tgcgctcatc
    22561 aggattaggg gtttttatcg gcatgatccc cggtgcgggg gcggatattg cagcttttgt
    22621 tgcctataac gaaactcgtc gtttcagtaa gacaccagaa aactttggta agggtgaaat
    22681 taaagctgtg gcctcctgtg aggcaggcgc gaatggctgc accggtggcg cattgttacc
    22741 tatgctgacg ctggggatcc ctggcgatgc ggtaaccgcc atcatgctgg gcgcgttaac
    22801 gttacaggga atgcaaccag gcccgctgat gtttaccgac cacggcgata tggtttatac
    22861 actgtttgtc ggcatgatct tttgttactt catgctgcta gttcttggac tgctctcttt
    22921 gaaagtcatc ggtaacgtgg tgaagatccc cggcaatatt ctcacaccga tgatcctcgc
    22981 actttgtgtg gtcgggactt atgcgttgaa caatagcctg tttgatgttg gcattatgct
    23041 gattgcaggc gtggtgggct atttcatgca gaaaggagga tatccggcat cacccgtagt
    23101 gctggcattg attatgggcc cgatggcaga aagtaatttt cgccgtgcgc tgtcgctttc
    23161 agggggttca ctggactttc tgtatacccg accgatcact ctggcattgc taactctggc
    23221 agcctttacg ctactgacgc caataatccg caaaataatg cgtttacggc gtcagtaact
    23281 gatatcgctc cccgaagttg gggagcgttt tttatttcag cagacgacgc gccgcttcca
    23341 ccacgatttt caccgcatgg ctctcggttt gtttcatcgt ctcagcattc gggatctctt
    23401 gctgggtgcg gttaacgata acccccgcta ccataccggc acgcaggccc tgacttgcac
    23461 acatggtcag cagggttgca gattccattt catagttcat tacgcccatc gcctgccact
    23521 cttccataga acctttaaag tgacgaacta cgcgaccaga gtaagtatcg taacgttcct
    23581 gacccgggta gaaggtatca gaagaagccg tcacaccaac gtgagtggtg gcaccaattg
    23641 acttcgccgc ttcaaccagc gcagtcgtac attcgaaatc agcgacagcc gggaattcca
    23701 gcggtgcgaa gtgcaggctc gcgccgtcca gacgaacaga cgccgtggta accaggacat
    23761 cacccacatt aatatgcggc tgaatagcgc ccgttgtacc gatacgcagg aaggtgcgaa
    23821 tgcccagctg tgccagctct tcaacagcaa tagaggtaga cgggccgccg ataccggtag
    23881 agcagacgat aacaggttta ccatccagct ctgcacgcca ggtggtgaat tcgcggtgag
    23941 atgccagctt aaccggctta tccatcagcg cggcgatctt ttccacacga tccgggtcgc
    24001 cagggacgat ggcaagcgta gccccttgta aatcgttttt agtgaggccg agatgaaaaa
    24061 catcagactt ggacatatac aactcctctg tgaatcggtt tagtcagaag aagcaaaaag
    24121 acactttacc gaagggttta acattttttc gtgatactca tcaccatgac gcaaatgcgt
    24181 tgcataaatt tacccatcaa aagtgatgca aatcacataa atcactcttc attctgcagg
    24241 actgcataaa agatgagccg attcaggcaa tgtgatttgt tgcatgctga ctatagttaa
    24301 cattgcgcaa catttttata agggtacgga gaataccatg gcaacaacac aacaatctgg
    24361 atttgcacct gcggcatcgc ctctcgcttc gaccatcgtt cagactccgg acgacgcgat
    24421 tgtggcgggc ttcacctcta tcccttcaca aggggataac atgcctgctt accatgccag
    24481 accaaagcaa agcgatggcc cactgccagt ggtcattgta gtgcaggaaa tttttggcgt
    24541 gcatgaacat atccgcgaca tttgtcgccg tctggcgctg gaggggtatc tggctattgc
    24601 acctgaactt tacttccgcg aaggcgatcc gaatgatttt gccgatatcc ccacgttgct
    24661 tagcggtctg gtagcaaaag tgcctgactc gcaggtgctg gccgatctcg atcatgtcgc
    24721 cagttgggct tcccgcaacg gcggcgatgt tcatcgttta atgatcaccg gattctgctg
    24781 gggtggacgt atcacctggc tgtatgccgc gcataatcca cagctaaaag ccgcagtggc
    24841 gtggtacggc aaactgacgg gcgacaagtc gctgaattca ccgaaacaac ctgttgatat
    24901 cgcaaccgat cttaacgcgc cggttctcgg cttatatggt ggtcaggata acagcattcc
    24961 gcaggagagc gttgaaacca tgcgccaggc gctgcgggct gctaatgcga aagcagagat
    25021 tatcgtctac ccggatgccg ggcatgcatt caacgccgat tatcgcccga gctatcatgc
    25081 cgaatctgcg aaagatggct ggcagcgtat gctggagtgg tttaagcagt atggtgggaa
    25141 gcggaattag tttcctctta gtctatattt gagatggtga gtgaacgagt gcattttgct
    25201 gccatctctg tcccccaaaa aataatcagt atgaattgat tggagagcac gactccgcta
    25261 tttgacctta agaaagtcgg caagtcatag tcataaatga gtctcttcac tggcagacac
    25321 cttagagatt taccatcaac tcccattctc aaacaaacat taattattat caacacattc
    25381 acattaatta taaattatct cagaccttcc tgataaaaag atattaaagt gaaacatttt
    25441 gtttttcaca ttttagttca tcccttttgg gcgtaaataa aggtaaaaaa taacaatact
    25501 atttttcttt cacaaaagaa ttaacaaaag gtttttcaag gatgagtgat attatttacc
    25561 tgaccataac cggagaacaa caaggatgta tttcctctcg ttgcggaacg agcgcatcaa
    25621 taggtaatcg ctggcagatt ggccatgaag atgaaatctt cgcgttctcg ctatctaata
    25681 gtattacgaa tacaggcaaa gggtcacaat tacatggcct tagtttctgt aagttgattg
    25741 acaaaagctc cccgttactt attaacgcta ttaacaataa cgagcagttg ttcatggagt
    25801 ttgacttcta tcggatcaat agatttggtc gatgggagaa atattacaac attcaactcc
    25861 gcggcgcgct cttatcagca atcaatcacc tgtttactga gaacaatctg gatacagaaa
    25921 ccatcactgt cagttatgaa tatatactct ccaggcatct gattgctaat actgaattta
    25981 gtcatttggc atttcccgat aactacaacc gtctgtttat accacggcaa aaaactaaag
    26041 atgataatgg cctcaaaaca ctcaatagta aagcggttgg cagactgctt gctgctggcg
    26101 gcatatacaa tgggaatatc gaaggattca gggaaacagc aaacaagttg ggaggagatg
    26161 cacctgctgg ttacgatcaa gttatggata acaaagggtt actcatcgct ggtgcttcaa
    26221 tagttgctgg agttgttctg ggaagaatga gattccctga tttggaatca tttgagcatt
    26281 tcggagccag aggagcagta tcaggaagac catttaagcc cgaccttgcc ggtggtccaa
    26341 tagaaaacct gacgatagat ggcgtaacaa tcaataggga aggtatcgct attgttgaaa
    26401 agcatatctc cagatttgat catgatccag ctaatgatgt aatgatttca aggctaaaaa
    26461 aaatagcaaa taaagaattg cttcctgaaa aatacgatct gaactactat actcatgaat
    26521 gccgcgagta tcagcgttat tgtaatttag gatgggaaac aggtgaaccg aatggtttag
    26581 atggatatga attatggaat aatgcacata ctgcaactct agaagacttt aaaattaaag
    26641 atactgactt atttcatccg gatgcaaaaa aatgacgatt acgatagttg aatttaacgt
    26701 gctgaaagtt atggctgaga aagatattga ctggagctgg atggtgctgg atcgtacttt
    26761 agctatacga aatattccag gatttggcaa cgtagcgaat atcgtaacaa agctggttaa
    26821 tcatgggttg gttgatatcg taaatggtga gggcaactct aaaccacgct acagagtttc
    26881 tcaatatgga ttgaatctta tcaaagaaca acaagacaac ctcttctgat ccagccatta
    26941 caggagcatg atttacattg tgatctttca ataagatact catcaatttt ggtacagaat
    27001 atcgtgatca ctaaaaatga acttaacgtg ctgaacgtta tgactgagaa agatataaac
    27061 tggaactgga tgaatctcga tcgcacacta tccataaggc aaataccagg attcggtaat
    27121 gttgttaata ttgttaacaa attagctaac gaaggtttgg ttattattga ggatagcggg
    27181 cataagtcta tgcctcacta ccacgttagt gaaaagggat acaatctcgt gcaacgtgag
    27241 aataaataac gtagcgttga ttactgaacg gaatcactca aaactgtgtt ttgcgaaaaa
    27301 aacgattcgg ttatttctac tcctgaagat ctgcttcaat ctggcactca tgcaaaatgg
    27361 cttccagtac tggaagccat tatcgtactg caatttacaa tgactcgtta atactcatca
    27421 tccccgacgc aaattctgcg ccgcctgcac catgttcgcc agtgccgcgc gggtttctgg
    27481 ccagccgcgc gttttcaggc cacagtccgg gtttacccac agacgctctg ccggaatgcg
    27541 ttttgccgct ttcttcagca gggcttcaat ccattccacg ctcggtacgt ttggcgagtg
    27601 aatgtcatag acgcctggac caatttcatt tggataatca aactcttcaa acgactccag
    27661 caactccatg tcggaacgcg aggtttcgat ggtgatgacg tctgcgtcca gcgccgcaat
    27721 cgaatccatg atgtcgttga actcgcagta acacatgtga gtgtggattt gtgtgtcatc
    27781 tttcgccacg gcggcgttga tacggaaagc ctccacgccc cactggagat aggcatccca
    27841 gtcgctgcga cgcagcggta aaccttcgcg cagtgccggt tcatcaatct ggatgatgcc
    27901 aattccagcg gcttccaggt ccgccacttc atcacgcagc gccagcgcaa tttgtttggc
    27961 gatggtttca cggctgacat cttcacgcgg gaatgaccag cagagtatgg ttaccggccc
    28021 cgtcagcatc cctttcaccg gtttgtcggt cagcgattgt gcatatttcg cccactccac
    28081 ggtaattggt gccgggcggc taacgtcacc aatgacaatc ggtggcttca cgcagcggga
    28141 accgtagctc tgtacccaac cgttttgcgt aaagacaaag ccatccagat gctcgccaaa
    28201 gtattccacc atgtcattac gctcggcctc gccatgtacc agcacatcca gtcccaaacg
    28261 ttcctgctcg acaatggcct gcttgatatg ttccgcaatg cccgtgcggt agttgttggc
    28321 gtcgagatta ccctttttga aatccagacg cagtgtacga atttccgtgg tttgcgggaa
    28381 ggaaccaatc gtggtagtcg gccacgcggg cagtttaaaa cgcgcacgct gggcttcagc
    28441 acgcacttca tagacattcg cacgctgact gtcctgggcg gtaatggctg caagacgctt
    28501 ttctaccgcc ggattatgga cacgggtaga gtgacgacgc gcctgaatcg gagcgctcca
    28561 ttctgccaga gccgccgtat caccactgtt cagcgcatcg cgcagcagtg ccagttcatg
    28621 gcatttttgt agggcgaagg caaaccagct tttcacttct gcatcaagac gcgtttccac
    28681 gctcaggtcg atggggctgt gcagcaacga gcaggaagat gccacccaca aatcgcgttt
    28741 gccgacaatg tccttaattt gcgcatattt ctcggtaaga tcggcgcgcc agacgttacg
    28801 accattgatc agacccgcag acagtagcca gtcagaaggc agacgcttgt gcagttcagc
    28861 aacgtcatct ttaccatgta caaggtccac atgcagcccc tgaacaggca gcgcagtaat
    28921 cgtgtcgaga tttggcgtta cgccttcaaa ataggtagtc agcagcagtt tcacctggcc
    28981 ctggagcgcg tcgtaagcgg gtttgtatgc gtccagccac gcctgcggta gttccagtac
    29041 cagcgctggt tcgtcgatct gtacccactc aatgccgcgt tttgccagtt ctgccaacac
    29101 ttgctgataa accggcagaa tgtcgttcag caggctcagg cgatcaaact gttcaccttt
    29161 cactttaccc agccacaggt aggtaaccgg ccccagcagc acgggcttga ccttgtggcc
    29221 cagcgccagc gcctcgtcca cttcctccag cagctgcgtc caggtcagtt tgaactgttg
    29281 gcctttaacg aactccggca ccatgtagtg atagttggtg ttaaaccatt tggtcatttc
    29341 cgctgccgcc gcaggttcgc cagtcggtgc acgtccacga ccaatacgga acagggtgtc
    29401 gatatctacc gaaccatctt tgttctgatg acgtggcgga acgttaccca gcagcagact
    29461 ggtggtcagt acatgatcgt accaggcaaa atcgcccacc ggcagcaggt cgatacccgc
    29521 ctgcttttgt tgatcccagt gacgggcacg taattcacgc ccaacagcca gcagttcttc
    29581 acgcgtggag ttccccgccc aataactctc ttgcgctttt ttcagctcgc gacgcaggcc
    29641 aacgcgaggg aaaccgaggg tgtgattaag aattgtcatt atatgcccct tatggaattt
    29701 ttagtattta gccgtccaga tgtttacaca tccataattg gcggttactg tatattcctc
    29761 aagcgcaaat tattcatgcc gaagtgaagg actttcatga tcgaagtaaa acacctgaaa
    29821 acgctacaag cgttgcggaa ctgcggctca ctcgcagccg ctgcggcgac gttgcatcaa
    29881 acgcaatccg ccctgtctca ccagtttagc gatctggaac aacgccttgg cttccggcta
    29941 tttgtgcgta aaagccagcc gcttcggttt acaccgcagg gagaaatcct gttgcaactg
    30001 gcgaatcagg tgctgcctca aatcagccag gcactgcaag cctgcaatga accgcagcag
    30061 acgcgcctgc gcattgccat tgagtgccat agctgtattc agtggctgac gcccgcgtta
    30121 gaaaatttcc ataagaactg gccgcaggtg gagatggatt ttaaatcggg cgtgacgttt
    30181 gacccgcagc ccgcgttgca gcagggtgag ctggatctgg tgatgacctc cgatattctg
    30241 ccgcgcagtt gcctgcatta ttcgccgatg ttcgattatg aagtgcgtct ggtgttggca
    30301 cctgaccatc cactggcggc gaaaacgcga atcacgccgg aagacctcgc cagcgaaacg
    30361 ctgttgattt atccggtgca gcgtagccga ctggatgtct ggcggcattt tcttcagccc
    30421 gcaggcgtta gcccgtcact gaaaagcgtc gataacacct tgttgttgat tcagatggtt
    30481 gccgcgcgga tgggcatcgc tgcgctaccg cattgggtgg tggagagttt tgaacgccag
    30541 ggtctggtgg tcaccaaaac cctgggcgaa ggcttgtgga gccgactgta cgccgccgtg
    30601 cgcgatggcg agcagcgtca gccgattaca gaagcgttta ttcgttcagc gcgcaatcac
    30661 gcctgcgatc atctgccgtt tgtgaagagc gcggagcgac ccacttacga tgcacccaca
    30721 gtgaggccag gatcaccagc gcgcctgtaa taaacgttgg ccagtgcggc tgttggtgcc
    30781 agatagccag gttaaccaac agccctgccg gaacgtgcat attattcata atgcccagcg
    30841 ttccggcgtc cacctgagtc gcgccgtagt tccacataaa gtagccaata ccggaagcca
    30901 ccacgccaag aaacaccaga atgccccatt gcagcgtggt ttgcggcatt ttctgcgcat
    30961 ttcccagcaa aaaccatgca atcaccgcca ccaggaacgc accaagatag aaccacgcaa
    31021 aggcgttatg ctgtggcatc ggacgagttt ccatcaggcg tttgtaaccc accatgccga
    31081 tggcaaaagt gatattggag agttgcacca gcagcaggcc agtccagaag tggtcggtga
    31141 cctgatcata gcgaataatt ccggcaccaa tcaccgccaa caaggcgcta aaagcatagc
    31201 cccagcgcag acggcgctta ctcatgatgt cataaatcag cgtgatgtag agcggagtca
    31261 gtacggtgaa cagcaacagt tcggagaccg tcaggtagag ataggcgcga aaactcagca
    31321 tatacatcac accgagctgc atcgcgccca ccagcatata caggccaacc gttttcaggc
    31381 tattgccacg ggtacgcaga aacggcagaa acacgagtgc cgccaggcca acgcgcacca
    31441 gcactgcaaa atagctatcg acgtgccccg caaggtactc gccataaaag ctaaaggaga
    31501 aggcccacag aatcgtggtg atgataagta gcgccacaat ggaaaactct ttgattaacg
    31561 agtatccatt gtagcgaagt atcaggttga caactgacca aataaagaac gattacgata
    31621 aatagagttt acgcagataa tgcggcaccg cgtcttcggc attagtacca atcacttcca
    31681 gctccggatg cagatctttc agacgctggt gcgcactgcc cataatgcag cctttacctg
    31741 ccatcgacag catttcggcg tcgttcatcc cgtcaccaaa ggcaatacaa tctttcagac
    31801 tgtagcccag tttcttcgcc accgcttcca gggcatggcc ttttgaaacg ccgcccgcca
    31861 tcacttccag acaagttagg gtagagaagc tgacgttgac gcgatcgccc caacgagcgt
    31921 tgattgcctg ctccagcggc agcagttgct catgagaatc gcaggtgaag aacactttgc
    31981 tgacgccttc cggctccagt aatcctggct catacagcgc atatttgaac actgcttctt
    32041 taaaaaagcg catctcttcc gggcggtggc gattcataaa ccattcgtcg tcgcgataaa
    32101 cgttagtaat gatgtccggg ttgtcgttga ccacgccaaa cagatcgctg gcaatgtcgc
    32161 gatccaggtt atgagcaaaa atcagattgc catccagatc gtgaacacgc gcgccattgg
    32221 aggtaatcat gtaagactta atctccagat tatcgcgaat ttgccctaca tcaacgtggt
    32281 ggcgaccggt cgcaaacaca aagttaatgc cgcgcgcggt gagcagcttc agagtttctt
    32341 tggcgtaagg ggataacgta tggtcgggag aaagtaacgt gccatctaaa tcagacgcaa
    32401 caacctggta cataagaaat ttaaacctct gtagagcggt ttccgctggg tgagttatgc
    32461 ctgttgaaaa aatcaacaat ggcgtggagc gcgactgagc gcattgcgtc cttttcaaaa
    32521 aggatctcat ggtacgcacc tttaattacc agcggtcgtc ccccttcgac aggatggccc
    32581 gcagcggtgc ggagttcaca aaaacggtcg tgcatgcggt tatccaccac gcgttcctct
    32641 tcagcctgca agagaagcgt tggcgtggcg tcatcacccg caccggctaa cacctgttcg
    32701 ccagccagaa tactttcgcg tacccaatgg taggtcggcc caccgacgcg aatcgttggg
    32761 tcatcagcat aaaagcgtaa gttacgtcga tatcgctgtc tgctgtgggt cagtacgttg
    32821 atagcaaacg gcaacgcgcg ccagcgcccg gtgcctattg catagccatc acggaaacgt
    32881 ggatgcgctt cggcccagtt aaggatctgc cgcgccataa atgacggcat acgaatcacg
    32941 atcccaaaca ttggcgcagt tagcgcaatg gcgtcacata cacctggatg gcgttgcaga
    33001 aataacgtgg agatcgcacc gcccatcgaa tgtgccagta tatagcgctt acgccacgga
    33061 ccaggctgga cctcctgttg ccagaatgcc gccaggtcat cgacataatc attaaagcga
    33121 ttaacatgcc caagatgcgg atcggctaac aggcgaccgg aacgtccctg cccgcgatgg
    33181 tcgatgatta agacatcaaa ccccaaatgg aacaggtcgt aggccagttc cgcatatttt
    33241 acgtagctct caatacgccc cgggcagatg actatcaccc ggtcatggtg ctgtgcgcga
    33301 aaacggacaa agcgcaccgg aatgtcatct acaccagtaa actctgcttc atcacgctga
    33361 cgccagaaat cagtcagcgg tcccatggta aaagcagcaa acgcgttttc tcttgtttcc
    33421 cagtcttttt gctgctgaaa catcgggtaa tctgcctctt aaaccacgta aaatcgtttt
    33481 tttaagcgtg cctgacacaa cgcttcgaca gtagcgtatt gtggcacaaa aatagacaca
    33541 ccgggagttc atcatgacct tagaatggtg gtttgcctac ctgctgacat cgatcatttt
    33601 aagcctgtcg ccaggctctg gtgcaatcaa caccatgacc acctcgctca accacggtta
    33661 tcgcggcgcg gtggcgtcta ttgctgggct tcagaccgga ctggcgattc atattgtgct
    33721 ggttggcgtg gggttgggga cgctattttc ccgctcagtg attgcgtttg aagtgttgaa
    33781 gtgggcaggc gcagcttact tgatttggct tgggatccag cagtggcgcg ccgctggcgc
    33841 aattgatctt aaaccgctgg cctctactca atcgcgtcga catttgttcc agcgcgcagt
    33901 ttttgtcaat ctcaccaatc ccaaaagtat tgtgtttctg gcggcgctat ttccgcaatt
    33961 catcatgccg cagcagccac aactgatgca gtatatcgtg ctcggcgtca ccactattgt
    34021 ggtcgatatt attgtgatga tcggttacgc cacacttgct caacggattg cgttatggat
    34081 aaaagggccg aaacagatga aggcattgaa taagattttt ggctcgctgt ttatgctggt
    34141 aggagcgttg ttggcatcgg cgaggcatgc gtgaaaaata atgtcggatg cggcataaac
    34201 gccttatccg acttactctg atgaagcgtt tggcatcaac gagaaataat caaatgaatg
    34261 ccaaatccgg caaataacgc cccggcaaaa ccatcaatcc acttcgccag acgttgataa
    34321 ccacggcgca tttgcggtag agcaaacagg ctggcaacca ccgtaaacca tgccagtgtt
    34381 tcgataataa tcaacgcgaa aatgccccag cgctcagtgg tgccgacgtt atcgccgaca
    34441 aacagcgaga ataccgaacc aaagtagata attgctttcg gattggcgag attggtcagt
    34501 aagcctttca ggaaactgcg cccgcttttt gccagttcta cctgtggcgc aggcgcagaa
    34561 acgacctctt ttttcagtgc gctacgcagc atctgataac ccatccagca gagatacagg
    34621 ccaccgccca ccataatcag cgtatgcagc caggccattt tttcgataat caaatgcagg
    34681 ccaagcaacg cgatccctgc ccagaccatt acgccgcagg taatgcccag cacgcccatc
    34741 atcgcctctt tacgggaacg actgacagcg gtctgagaaa caaagaagaa atcgggaccg
    34801 gggctcataa gcgccacaat gtgcaccatg gcaacggtga ggaataacat caacattcaa
    34861 ttgactcgcg gagaaatcgt ttacggagtc ttcatcctgg cactttttta tgctgctgac
    34921 tactcttcgt catcgccatc gacatgcgca cgaattagcg ccataaacgg tttgccaaag
    34981 cgttccagct tgcgcatccc aacgccgtta acgctgagca tttcgctggc ggagatcggc
    35041 atctgttccg ccatctcaat caaggtcgca tcgttaaaca ccacatacgg cgggacatta
    35101 ctttcatcgg caatcgattt acgcagtttg cgtaatttgg cgaacagttt gcgatcgtaa
    35161 ttgccaccga aagatttctg cattgctttc ggtttgagcg ccacaatacg cggcacggca
    35221 agttgtaaag aggattcgcc gcgcaacacc gggcgtgcgg cttctgtcag ttgtaatgcc
    35281 gaatgctggg cgatgttttg cgtcaccaga ccgagatgaa tcaactggcg gattacgctc
    35341 acccagtgct catgactttt atcgcggccc atgccgtaga ctttcagttt gtcatgacca
    35401 tagtcgcgga ttcgctggtt attggcgccg cgaatcactt ccactacata gcccatccca
    35461 aaacgctgat tcacacgacc aatggtggaa agcgcaatct gcgcatcggt tgaaccatcg
    35521 tattgtttcg gcggatcgag gcagatatcg cagttcccgc acggctcctg ccgcccttcg
    35581 ccaaagtagt tcagcagcac cagacgacgg caagtttgcg cttcggcaaa agcgcccatc
    35641 gcattgagtt tatggcgctc gatatcctgc aactgcccct gcggcttctc ttccagacaa
    35701 cggcgcagcc acgccatatc agccggatcg taaaacagca tcgcttccgc gggcaggcca
    35761 tcacgcccgg cgcgaccggt ttcctgataa taggattcga tattgcgtgg aatatcaaag
    35821 tgaaccacga agcggacgtt aggcttgttg atgcccatgc cgaacgctac cgtcgccacc
    35881 acaatttgca ggtcatcacg ctggaatttt tcctgcacgt ccgcacggac atcgttttca
    35941 agcccggcgt gataagccgc tgcgctgata cccttgcttt gcagacgcgc ggcggtgtct
    36001 tctactttcg cacgactatt acagtaaata atccccgact taccgcgttg ttcctgtaca
    36061 taacgcatca actgatcgag cggtttgaat ttctccatca gcatgtagcg aatattcgga
    36121 cggtcaaaac tgctaatttg aatcagcgga tcgttcagcc ccagcaggcg cacgatatcc
    36181 tgccgcgtgg tgtcgtcggc ggtggcggtt agcgccataa acggcagcgt cgggaaccgc
    36241 tggcgcaact gaccgagcgc ggcatattcc gggcggaaat cgtggcccca ttgggagata
    36301 cagtgcgctt catcaacggc taataacacc ggattccagt gcgccagatg ctcaagaaag
    36361 ttatccagca tcaggcgttc cggggcgata taaagcagac gaatttgccc ggtgcgacag
    36421 cctgtcatca cttcaagctg ctgctcgcgg gtttgcgtcg agttaaggca cgccgccgcc
    36481 acaccgttgg cctgtagctg atccacctga tctttcatca acgaaatcag tggtgaaaca
    36541 accacggtaa gaccgtttag cagtaaggca ggaatttgat agcaaaggga ttttccgcca
    36601 ccagtgggca tgacgaccag gcaatcgcga ccggaaagta cggtgtcgat aatctcttcc
    36661 tggccggggc gaaactgttg gtagccaaag gtttcttgta aaacctgttt agctccggac
    36721 tccagattca acacttccgc ctgcgccaca ttaaccccga ttgcctgaaa taaaaacagg
    36781 cgctattttc agcgcctgcg cgagaaactg caatgctttt agtctctgag agggtaatcc
    36841 tggatactgg attccagttt tgcctttcaa tcaactcaca ttagaacaag tcgttcagca
    36901 taacccccac acccacacgg gtctggttaa agttatagtc gatgagcgat tcgccataac
    36961 cgctgtaaac ctgggtataa aggcgcacat gcctggtgat cgggtaactt aagcctaact
    37021 ccgcaccacc gtaaccggtg ttccagttgt actgtccttt cgcactcagc accgcatcac
    37081 ccaggtgata gccgatttta agctggtagt aacccatata tttggtgata tctgggttat
    37141 cgtcagtatt ccccaccaca taccacggct tcacctctac cagccagtta ccgttttctg
    37201 ccatcagacg ggtataaagg cggttccagc tgcgggaagt cgggtctgaa cgcccgttag
    37261 agtcgtggtt ataacccatc tccacatcgc gcagcgtcca gccagcaaag cggtaatcgg
    37321 tggcaaaacc aaggaacaat tgcggttcgt agttggtttc acgaaacggt gaagactctt
    37381 cgctattgga cagttgccac caggattttt gcgtgtaaga agcccccaac accgagtttg
    37441 gccccaatat tccgcgccac agcggaaatg ccaggctcaa ctggaatttc acttcatcct
    37501 tacgcgcgtt ttccgcccag tcataactgg cgatcgcttc tttgttcaga tcgctggttt
    37561 gggtgtagat gaggtagttg gtgtcataag gatagagcgt gaaaggattg tcatgctcct
    37621 gcagcatatt cgcaataata ctgccacgca ccgcaggcgc gtcatgcacc tcttttaccg
    37681 ttgcctcttg cgcatacact gccattggca acataaacac cggcaacaac cagccctgca
    37741 gagtccgcat cggtcgtgtt ctccgtaacg aattattgat tagatgaata tttttctgcg
    37801 ggctatttta catacttaac tcctttgccg ttagttatcc cttcctaaag tagaaaacca
    37861 caattaagaa gcataaaatc aacatttcat taaccaatga ggtgtaagga tgatatgtct
    37921 gccgtactga ccgctgaaca agccctgaaa ttagtgggtg agatgtttgt ttatcacatg
    37981 ccatttaacc gcgcattggg gatggaactg gagcgttacg aaaaagagtt cgcacagctg
    38041 gcctttaaaa atcagccaat gatggtgggt aactgggcgc aaagcatttt gcacggcggg
    38101 gtcattgcgt cggcgctgga tgtcgccgca ggtctggtgt gcgtgggaag taccttaacc
    38161 cgccacgaaa ccatcagtga agatgaacta cgccagcggc tatcgcggat ggggaccatt
    38221 gatcttcgcg tcgattatct gcgcccaggc aggggcgagc gttttactgc taccagtagt
    38281 ctgttgcgtg caggcaataa agtcgccgtt gcccgcgttg aattacacaa tgaagaacag
    38341 ctttatatag ccagtgccac cgccacctat atggtaggtt gataacgtaa aagcaggtaa
    38401 actgctttta ccaataaaaa atgagttttg ccaatggaca ccaaacaaac gcggcagggc
    38461 gtattactcg ctcttgccgc ttattttatt tggggtattg cgccagcgta cttcaagctg
    38521 atttactacg tgcccgccga tgagatcctg acgcatcgcg tgatctggtc gtttttcttt
    38581 atggtggtgc tgatgagcat ttgccgccag tggtcctatt taaaaacgct gattcagaca
    38641 ccaaagaaaa tcttcatgct ggcagtctct gccgtgctga ttggtggcaa ctggctactg
    38701 tttatctggg cggtgaacaa tcaccatatg ctggaagcga gccttggtta ctttattaac
    38761 ccgctggtga acattgtgct ggggatgatt ttcctcggcg agcgattccg ccggatgcaa
    38821 tggctggcgg tgattctggc gatatgtggt gtattggtcc agctctggac ttttggttcg
    38881 ctgccaatca tcgcgctggg gctggcgttt agttttgcct tctacggcct ggtacgcaag
    38941 aaaattgccg ttgaagcgca aaccggcatg ttaatcgaaa ccatgtggct gctgcccgtg
    39001 gcggcgattt acctgtttgc tattgccgac agctcaacca gccatatggg gcaaaacccg
    39061 atgtcgctga atttactgct gatcgccgcc ggtattgtca ccaccgtgcc actgttatgt
    39121 tttaccgcag cagctacacg cttacgactc tcaacattag gctttttcca gtacattggg
    39181 ccgacgctaa tgttcctgtt ggcagtgacg ttttatggtg aaaaaccggg tgccgataag
    39241 atggtgactt tcacctttat ttgggtggcg ctggcaattt ttgtgatgga tgcgatttat
    39301 acgcagcgta gaacggccaa ataaaaaata aatatattct gaaaaaatat gccgactctt
    39361 gcatgagagt gggcatattg ccatgctgac aggagactca cataaggaaa ggcagaattt
    39421 ccagacgtcg ttgtatattt tcatttgctt taataaattt catttttggt aaattcagca
    39481 ttatgttatt ag
//
LOCUS       DAZGAP010000015        39171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGAP010000015 DAZGAP010000000
VERSION     DAZGAP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.015"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..1751)
                     /gene="glnS"
                     /locus_tag="ACHLAM_000708"
     CDS             complement(87..1751)
                     /gene="glnS"
                     /locus_tag="ACHLAM_000708"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO7852290.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSCEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1954..3900)
                     /gene="nagE"
                     /locus_tag="ACHLAM_000709"
     CDS             complement(1954..3900)
                     /gene="nagE"
                     /locus_tag="ACHLAM_000709"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO7852291.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4233..5033
                     /gene="nagB"
                     /locus_tag="ACHLAM_000710"
     CDS             4233..5033
                     /gene="nagB"
                     /locus_tag="ACHLAM_000710"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7852292.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5093..6241
                     /gene="nagA"
                     /locus_tag="ACHLAM_000711"
     CDS             5093..6241
                     /gene="nagA"
                     /locus_tag="ACHLAM_000711"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7852293.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            6250..7470
                     /gene="nagC"
                     /locus_tag="ACHLAM_000712"
                     /gene_synonym="nagR"
     CDS             6250..7470
                     /gene="nagC"
                     /locus_tag="ACHLAM_000712"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO7852294.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7518..8270
                     /gene="nagD"
                     /locus_tag="ACHLAM_000713"
     CDS             7518..8270
                     /gene="nagD"
                     /locus_tag="ACHLAM_000713"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO7852295.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8526..10190
                     /gene="asnB"
                     /locus_tag="ACHLAM_000714"
     CDS             8526..10190
                     /gene="asnB"
                     /locus_tag="ACHLAM_000714"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO7852296.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10571..10647
                     /locus_tag="ACHLAM_000715"
     tRNA            10571..10647
                     /locus_tag="ACHLAM_000715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10605..10607,aa:Met,seq:cat)
     gene            10656..10740
                     /locus_tag="ACHLAM_000716"
     tRNA            10656..10740
                     /locus_tag="ACHLAM_000716"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10690..10692,aa:Leu,seq:tag)
     gene            10764..10838
                     /locus_tag="ACHLAM_000717"
     tRNA            10764..10838
                     /locus_tag="ACHLAM_000717"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10796..10798,aa:Gln,seq:ttg)
     gene            10873..10947
                     /locus_tag="ACHLAM_000718"
     tRNA            10873..10947
                     /locus_tag="ACHLAM_000718"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10905..10907,aa:Gln,seq:ttg)
     gene            10963..11039
                     /locus_tag="ACHLAM_000719"
     tRNA            10963..11039
                     /locus_tag="ACHLAM_000719"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10997..10999,aa:Met,seq:cat)
     gene            11088..11162
                     /locus_tag="ACHLAM_000720"
     tRNA            11088..11162
                     /locus_tag="ACHLAM_000720"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11120..11122,aa:Gln,seq:ctg)
     gene            11200..11274
                     /locus_tag="ACHLAM_000721"
     tRNA            11200..11274
                     /locus_tag="ACHLAM_000721"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11232..11234,aa:Gln,seq:ctg)
     gene            complement(11428..12603)
                     /gene="ubiF"
                     /locus_tag="ACHLAM_000722"
     CDS             complement(11428..12603)
                     /gene="ubiF"
                     /locus_tag="ACHLAM_000722"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO7852297.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            12749..14173
                     /gene="miaB"
                     /locus_tag="ACHLAM_000723"
     CDS             12749..14173
                     /gene="miaB"
                     /locus_tag="ACHLAM_000723"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO7852298.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14326..15366
                     /gene="ybeZ"
                     /locus_tag="ACHLAM_000724"
     CDS             14326..15366
                     /gene="ybeZ"
                     /locus_tag="ACHLAM_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO7852299.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15363..15830
                     /gene="ybeY"
                     /locus_tag="ACHLAM_000725"
     CDS             15363..15830
                     /gene="ybeY"
                     /locus_tag="ACHLAM_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO7852300.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            15920..16798
                     /gene="corC"
                     /locus_tag="ACHLAM_000726"
     CDS             15920..16798
                     /gene="corC"
                     /locus_tag="ACHLAM_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO7852301.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16823..18361
                     /gene="lnt"
                     /locus_tag="ACHLAM_000727"
     CDS             16823..18361
                     /gene="lnt"
                     /locus_tag="ACHLAM_000727"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO7852302.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(18439..20316)
                     /locus_tag="ACHLAM_000728"
     CDS             complement(18439..20316)
                     /locus_tag="ACHLAM_000728"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO7852303.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            20771..21679
                     /gene="gltI"
                     /locus_tag="ACHLAM_000729"
     CDS             20771..21679
                     /gene="gltI"
                     /locus_tag="ACHLAM_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO7852304.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21849..22589
                     /gene="gltJ"
                     /locus_tag="ACHLAM_000730"
     CDS             21849..22589
                     /gene="gltJ"
                     /locus_tag="ACHLAM_000730"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO7852305.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            22589..23263
                     /gene="gltK"
                     /locus_tag="ACHLAM_000731"
     CDS             22589..23263
                     /gene="gltK"
                     /locus_tag="ACHLAM_000731"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO7852306.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23263..23988
                     /gene="gltL"
                     /locus_tag="ACHLAM_000732"
     CDS             23263..23988
                     /gene="gltL"
                     /locus_tag="ACHLAM_000732"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO7852307.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24106..25041
                     /gene="rihA"
                     /locus_tag="ACHLAM_000733"
     CDS             24106..25041
                     /gene="rihA"
                     /locus_tag="ACHLAM_000733"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO7852308.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(25086..25568)
                     /gene="ybeL"
                     /locus_tag="ACHLAM_000734"
     CDS             complement(25086..25568)
                     /gene="ybeL"
                     /locus_tag="ACHLAM_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO7852309.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25803..28385
                     /gene="leuS"
                     /locus_tag="ACHLAM_000735"
     CDS             25803..28385
                     /gene="leuS"
                     /locus_tag="ACHLAM_000735"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO7852310.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28400..28981
                     /gene="lptE"
                     /locus_tag="ACHLAM_000736"
     CDS             28400..28981
                     /gene="lptE"
                     /locus_tag="ACHLAM_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO7852311.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28981..30012
                     /gene="holA"
                     /locus_tag="ACHLAM_000737"
     CDS             28981..30012
                     /gene="holA"
                     /locus_tag="ACHLAM_000737"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO7852312.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSHPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            30014..30655
                     /gene="nadD"
                     /locus_tag="ACHLAM_000738"
     CDS             30014..30655
                     /gene="nadD"
                     /locus_tag="ACHLAM_000738"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO7852313.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30679..31290
                     /gene="cobC"
                     /locus_tag="ACHLAM_000739"
     CDS             30679..31290
                     /gene="cobC"
                     /locus_tag="ACHLAM_000739"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO7852314.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            31550..31867
                     /gene="rsfS"
                     /locus_tag="ACHLAM_000740"
     CDS             31550..31867
                     /gene="rsfS"
                     /locus_tag="ACHLAM_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO7852315.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31871..32338
                     /gene="rlmH"
                     /locus_tag="ACHLAM_000741"
     CDS             31871..32338
                     /gene="rlmH"
                     /locus_tag="ACHLAM_000741"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO7852316.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            32369..34270
                     /gene="mrdA"
                     /locus_tag="ACHLAM_000742"
     CDS             32369..34270
                     /gene="mrdA"
                     /locus_tag="ACHLAM_000742"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO7852317.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            34273..35385
                     /gene="mrdB"
                     /locus_tag="ACHLAM_000743"
                     /gene_synonym="rodA"
     CDS             34273..35385
                     /gene="mrdB"
                     /locus_tag="ACHLAM_000743"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO7852318.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35396..36484
                     /gene="rlpA"
                     /locus_tag="ACHLAM_000744"
     CDS             35396..36484
                     /gene="rlpA"
                     /locus_tag="ACHLAM_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO7852319.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGLYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36624..37835
                     /gene="dacA"
                     /locus_tag="ACHLAM_000745"
     CDS             36624..37835
                     /gene="dacA"
                     /locus_tag="ACHLAM_000745"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO7852320.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37945..38208
                     /gene="ybeD"
                     /locus_tag="ACHLAM_000746"
     CDS             37945..38208
                     /gene="ybeD"
                     /locus_tag="ACHLAM_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO7852321.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            38309..38950
                     /gene="lipB"
                     /locus_tag="ACHLAM_000747"
     CDS             38309..38950
                     /gene="lipB"
                     /locus_tag="ACHLAM_000747"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO7852322.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         9589 a        10186 c        10051 g         9345 t
ORIGIN      
        1 gccgcagagc gcggcgtttg ggattgtttg catatttctt gccggatgtg gcgtaaatgc
       61 ctcacccggc ctggtagaac ttaaaactac tcgcctactt tcgcccaggt atcacgcagc
      121 cctacggtgc ggttaaatac cggtttttcg gcagtggaat ggcggctgtc gaggcagaaa
      181 taaccttcac gctcaaactg gaatgcttta cccgctaccg cgtctttcag cgacggttca
      241 gcgaaaccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca
      301 gcacctgggt taggcacgct gaacagacgg tcatacaaac ggatttcaac cggcagcgca
      361 tgtgccgcgc tcacccagtg aataacacct ttcactttac gaccatctgc cggatctttg
      421 cttaaggtgt cggcgtcata agtacagaag atggtggtga tattaccttc ggcatctttc
      481 tcgacgcgtt ctgccttaat cacgtaagca ttacgcagac gcacttcttt acccaacacc
      541 agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat ccaaatctca
      601 cagctaaacg gcacctgacg gctacccatt tccggtttgt tcggatggtt cggcatggtg
      661 accatttcgc cttcgccctg gtagttttcg ataaccagtt tcaccggatc gataaccgcc
      721 attgcgcgcg gggcattttc gttgagatct tcacggatgc aggattccag tgacgccatc
      781 tcaatggtgt tgtcctgctt ggtcacacca atgcgtttgc agaactcacg aatagaagcc
      841 gcagtgtaac cacgacgacg cagaccagaa atggtcggca tacgcgggtc atcccagcct
      901 tcaacgtgct tatcggtcac cagcaggttc agcttacgct tggacatcac agtgtattcc
      961 agattcaggc gcgagaattc gtactggcgc gggtgaacag gaatggtgat gttgtccagt
     1021 acccagtcgt acagacggcg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata
     1081 ccttccagcg catcgctgat gcagtgggtg aagtcgtaca tcgggtagat gcaccacttg
     1141 ttgccagtct ggtggtgttc agcaaattta atgcggtaca gcaccggatc gcgcattacg
     1201 ataaatggcg acgccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacta
     1261 ccggtacgca ttttttcgaa cagcgccagg ttctcttcaa cgctgcggtc gcggtacggg
     1321 ctgtttttac ccggctgcgt cagggtgccg cggtattcgc ggatctgttc cggtgtcagt
     1381 tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca
     1441 aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca ctcaacgtcg
     1501 tttttgatcg actcaacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc
     1561 aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca
     1621 tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc
     1681 ttaccactgg ccagatcttc atcgatgatc tgacgaataa agttagtcgg gcgggcttct
     1741 gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata
     1801 agcgggacag gctgacaact gttagttagt gaaaatcgaa aatctcactg agattatggg
     1861 gatgattgat gaaaaaaaaa gcggcagaga agatctccac cgcttgtcat tgttggatgc
     1921 gacgctcaag cgtcgcatca ggcataaagc agattacttt ttgatttcat acagcggtgt
     1981 ttgacccgcc acaacatggc cctgagcttt aatgatcagg ccgctgaaat cgtcgatatt
     2041 gctgcaaacc accgggctaa tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat
     2101 ttccagaatc ggttgtcctg cgcttacctg cgcgccctct tccaccagac gtttaaagcc
     2161 tttaccttcc agcgctacgg tgtcgatacc catatggacg acgatctccg cgcctttttc
     2221 ggtttccaga cagaacgcgt ggttggtgtt gaagattttc acgattgtcc ctgcggctgg
     2281 tgatacgacg attttatctg tcggtttcac cgccacaccg tcacccaccg ctttgctggc
     2341 gaatgcttcg tcaggaacct gatccagtgc cacaacatca ccggtaatcg gcgataccag
     2401 ctccgcgata gacaccgcgt ttggtacagc ctgcggtttt gctacaggcg cggcagttgc
     2461 aggcgcagtt tcagctgacg cagcggctac cggaccacgg gcaacgactt tcttcatcgc
     2521 atcgccgatg gattctgctt tcgcgcccac aatcacctga atagtctgtt tgttcagttt
     2581 tactacccca gaagccccca gacgtttaca catcacatcg ttgacgcgag ctgagtcaac
     2641 cacggtaagg cgcagacgag taatacaggc gtcaatggct ttcaggttgt cagtaccgcc
     2701 aaccgcagca atatagttgg ttgccagttg attgagacct tcttccgtgt tgctgttggc
     2761 ttcttcagta acgatctcgt cttctttatc ttcacggccc ggcgttttca ggttgaacat
     2821 gcggataacc aaactgaaca ccacgaagta gatagcgaag aagacaacac ccatcaccag
     2881 cagcatccag acgttctggc tggcggccgg caggttatac atcaacgcgt agtcgatagc
     2941 ccccgcagag aaggagaagc ccgcgtggat acccagcagc gttgccacaa acaggctgat
     3001 accggtcagc agtgcgtgca ggaggtacag cagcggcgca aggaacatga acaggaattc
     3061 cagcggctca gtcacaccgg tcaggaacgc agtaacagca acagaaagca gcataccgcc
     3121 aaccatcgga cgacgctctt tcggtgctgc aaagtacatc gccagcgccg cacccggcag
     3181 accgaacatc atgatcggga agaagccgga catgaacatc cccgcggtgc cgtcaccggc
     3241 atagaagcgg ttaatgtcgc cgtggaaaac cgtacccgcc gcgttggtga attcaccaat
     3301 ctggaaccag gcgatggtgt tcagtacctg atgcagaccg gttgggatca gcagacggtt
     3361 gatgaaacca aagataccgg aacccagcgc gcccgcagaa acgatccact cgccgcctgc
     3421 atggatagcg tgctgtaccg gcggccagac gtaaccaaaa atggccgcca gcaccaggca
     3481 gaagaagccg gtggcaatcg gcacaaagcg tttgccgccg aagaagctca ggaagtccgg
     3541 cagtttaata tcggaccaac ggttataggc tgcaccaccg accagaccgg taatgatacc
     3601 cgccagtaca cccatgttaa tttctgggtt gatggtcacc atcgctttgg ttaacacaaa
     3661 gtaacctacc gcacccgcca gtgccgccgc acctgcgctg tctttcgacc agctggatgc
     3721 cacgccgatg gcgaagatta atgcgaggtt atcaaaaatc gcaccgcccg cctgggcaat
     3781 aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc aacagcgccg ccaccggcag
     3841 caccgcgata gggagctgta acgccctacc tagtcgctgg aaaaaaccta aaatattcat
     3901 cttattcccc ctacgagaac cctatttggc tcgtttaaag ccgtattttt attttgctgc
     3961 aaattgtact gccgatgttc tgtaatcaga ttgttagatc atctgctaca gagtgtgtga
     4021 aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc accaaatatc
     4081 gttatcatca ctccctttta ctggctaaac cagaaaactt attttatcat tcaaaaaatc
     4141 aggtcggatt gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc
     4201 aacggcttac attttactta ttgaggtgaa taatgagact gatccccctg actaccgctg
     4261 aacaggtcgg caaatgggct gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccga
     4321 ctgccgatcg tccgtttgta ctgggcctgc cgactggcgg cacgccgatg accacctata
     4381 aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt gtcaccttca
     4441 acatggacga atatgtcggt ctgccgaaag agcatccgga aagctactac agctttatgc
     4501 accgtaattt cttcgatcac gttgatattc cagcagaaaa tatcaacctt ctcaacggca
     4561 acgccccgga tatcgacgcc gaatgccgcc agtatgaaga aaaaatccgt tcttacggaa
     4621 aaattcatct gtttatgggc ggtgtaggta acgacggtca tattgcattt aacgaaccgg
     4681 cgtcttctct ggcttctcgt actcgtatca aaaccctgac tcatgacact cgcgtcgcga
     4741 actctcgttt ctttgataac gatgttaatc aggtgccaaa atatgccctg actgtcggtg
     4801 ttggtacatt gctggatgcc gaagaagtga tgattctggt gctgggtagc cagaaagcac
     4861 tggcactgca ggccgccgtt gaaggttgcg tgaaccacat gtggaccatc agctgtctgc
     4921 aactgcatcc aaaagcgatc atggtgtgcg atgaaccttc caccatggag ctgaaagtta
     4981 agactttaag atatttcaat gaattagaag cagaaaatat caaaggtctg taattattat
     5041 ccctgccctg gctccttgct cagggcaata ttttttaaaa tcgggggtca gaatgtatgc
     5101 attaacccag ggccggatct ttaccggcca cgaatttctt gatgaccacg cggttgttat
     5161 cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca
     5221 acgttcactg aacggggcca ttctctcccc cggttttatc gatgtgcagt taaacggctg
     5281 cggcggcgtg cagtttaacg atactgctga agcagtcagc gtagaaacgc tggaaatcat
     5341 gcagaaagcc aatgagaaat caggctgtac taactatttg ccgacgctta tcaccaccag
     5401 cgacgagctg atgaaacagg gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa
     5461 tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca
     5521 taatccgaat tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt gtgagaacgc
     5581 cgacgtgatt accaaagtga ctctggcacc ggaaatggtt ccagcggaag tcatcagcaa
     5641 actggcaaat gccgggattg tggtttctgc tggtcactcc aacgcgacgt tgaaagaagc
     5701 gaaagccggt ttccgcgcgg ggattacctt tgccacccat ctgtacaacg cgatgccgta
     5761 tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc gacgaagctg acatttattg
     5821 cggtattatt gctgatggcc tgcatgttga ttacgccaac attcgtaacg ctaagcgtct
     5881 gaaaggcgac aaattgtgtc tggttaccga tgccaccgcg ccagcaggtg ccaacattga
     5941 acagttcatt tttgcgggta aaacaatata ctaccgtaat ggactttgtg tggatgagaa
     6001 cggtacgtta agcggttcat ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca
     6061 ttgtggtatc gcactggatg aagtgctgcg tatggcgacg ctctatccgg cacgtgcgat
     6121 tggcgttgag aaacgtctcg gcacgctcgc cgcaggtaaa gtagccaacc tgactgcatt
     6181 cacacctgat tttaaaatca ccaggaccat cgttaacggt aacgaggtcg taactcaata
     6241 agagaaagta tgacaccagg cggacaagct cagataggta atgttgatct cgtaaaacag
     6301 cttaacagcg cggcggttta tcgcctgatt gaccagtacg ggccaatctc gcggattcag
     6361 attgccgagc aaagtcagct tgcccccgcc agcgtaacca aaattacgcg tcagcttatc
     6421 gaacgcgggc tgatcaaaga agttgatcag caggcctcca ccgggggccg ccgcgctatc
     6481 tccatcgtca ccgaaacccg caatttccac gcaatcggcg tacggcttgg tcgtcacgac
     6541 gccaccatca ccttgtttga tctcagcagc aaagtgctgg cagaagaaca ttacccgctg
     6601 ccggaacgta cccagcagac gctggaacat gccctgctga atgccattgc tcagtttatt
     6661 gatagctacc agcgcaagct acgcgagctg atcgcgattt cggtgatcct gccagggctt
     6721 gtagacccgg acagcggcaa aattcattat atgccgcata ttcaggtaga aaactggggg
     6781 ctggttgaag cactggaaga gcgttttaaa gtgacctgtt ttgtcggcca cgatatccgc
     6841 agtctggcgc tggcagagca ctacttcggt gcaagtcagg attgcgaaga ctccattctg
     6901 gtgcgtgtcc atcgcggaac cggggccggg attatctcta acgggcgaat ttttatcggg
     6961 cgcaacggca acgtcggtga aattggccat attcaggtcg aaccgctggg tgaacgctgc
     7021 cactgcggca actttggctg cctggaaact atcgccgcca atgctgccat tgaacaacgg
     7081 gtgttgaatc tgttaaagca gggctaccag agccgtgtgc cactggacga ctgcaccatc
     7141 aaaactatct gcaaagccgc gaacaaaggc gatagcctgg cctcggaagt gattgaatat
     7201 gtcggtcgtc atctgggtaa aaccatcgcc attgctatca acttatttaa tccgcaaaaa
     7261 attgttattg ctggagaaat caccgaagcc gataaagtgc tgctccctgc tattgaaagc
     7321 tgcattaata cccaggcgct gaaggcgttt cgtactaatc taccggtggt acgttctgag
     7381 ttggatcacc gctcagcaat cggcgctttt gcgctggtaa aacgcgccat gctcaacggt
     7441 attttgctcc agcatttgct ggaaaattaa tgtgctttta tagtggcgct tattgttgtc
     7501 aatattctgg gtagtccatg accattaaaa atgtaatttg cgatatcgac ggcgtgctga
     7561 tgcacgataa cgtcgccgta ccgggtgcag cggaattttt gcacgggatt atggataaag
     7621 gcctgccgct ggtgttgctg accaactatc cttcgcagac cgggcaagat ctggcgaatc
     7681 gctttgccac tgccggtgtc gatgtaccgg atagcgtgtt ttatacctcc gcgatggcga
     7741 ccgccgattt cctgcgtcgc caggaaggca agaaagcgta tgtggtgggc gaaggcgcac
     7801 tgattcatga actgtacaaa gccggtttca ctattaccga tgtgaaccct gattttgtga
     7861 ttgttggcga aacgcgttcc tacaactggg acatgatgca taaagcagcc tatttcgtcg
     7921 ctaacggcgc acgctttatc gccaccaacc cggacaccca cgggcgcggt ttttatcccg
     7981 cctgtggcgc gttgtgtgca gggatcgaga aaatctccgg gcgcaaaccg ttctatgttg
     8041 gaaagccgag tccgtggatc atccgcgcgg cattaaacaa aatgcaggcg cattcggaag
     8101 aaacggtgat tgtcggcgat aacttgcgta ccgatattct ggcgggcttc caggccggtc
     8161 tggagacgat tctggtgctt tctggtgttt cgtcactcga cgatatcgac agtatgccgt
     8221 tccgcccaag ttggatttac ccatcggtcg ctgaaatcga cgttatctga atagcaacca
     8281 cgcctcaggg cgtggtttct tatttttacc catcattaaa atcaccccat tcaataaaac
     8341 ccgtcaaaaa ttatcgatcc atcaataaat atacccaaaa gccctcccct ttttcatcaa
     8401 tcagcaatcg ccattacgtt ttttattttt ccactgccaa atcgcttgcg caccgtgctg
     8461 ctttgcggca tttttttaac aagtaaacac aacacgcagt aaacaccagg ttaacggaga
     8521 aggttatgtg ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta
     8581 agaaagcact cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt
     8641 atgccagcga taacgccatc ctcgcccacg aacgtctgtc aattgttgac gttaacgcgg
     8701 gggcgcaacc tctctacaac caacaaaaaa ctcatgtgct ggcagtaaac ggtgaaatct
     8761 acaaccacca ggcattgcgc gctgaatatg gcgatcgtta ccagttccag accgggtctg
     8821 actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc
     8881 agggcatgtt tgcctttgcc ctgtacgaca gcgaaaaaga tgcatatctg attggtcgcg
     8941 accatctggg gatcatccca ctgtatatgg gctatgacga acacggtcag ctgtatgtgg
     9001 cctcagaaat gaaagccctc gtgccagttt gccgcacgat taaagagttt ccggcgggga
     9061 gctatttgtg gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact
     9121 acgatgcggt gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt
     9181 ccgttaaaag ccatctgatg tctgatgtgc cttacggtgt gctgctttct ggtggtctgg
     9241 attcctcaat tatttccgct atcaccaaga aatacgccgc ccgtcgcgtg gaagatcagg
     9301 aacgctctga agcctggtgg ccgcagttgc actcctttgc tgtaggtctg ccgggctcac
     9361 cggatctgaa agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc
     9421 acttcactgt acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt
     9481 atgatgtgac cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg
     9541 cgatgggcat taaaatggtg ctgtccggtg aaggttctga cgaagtgttt ggcggttatc
     9601 tatacttcca caaagcgccg aacgctaaag agttgcatga agagacggtg cgtaaactgc
     9661 tggccctgca tatgtatgac tgcgcgcgtg ccaacaaagc gatgtcagcc tggggcgtgg
     9721 aagcacgcgt tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccac
     9781 aggataaaat gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgaag
     9841 cgtatctgcc tgcaagcgtg gcctggcggc aaaaagagca gttctccgat ggcgtcggtt
     9901 acagttggat cgacaccctg aaagaagtgg cagcgcagca ggtgtctgat caacagctgg
     9961 aaactgcccg cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc
    10021 gggagatctt tgaagaattg ttcccgcttc cgagcgccgc tgagtgcgtg ccgggcggtc
    10081 cttccgtcgc ttgttcttcc gctaaagcga ttgagtggga tgaagcgttc aagaaaatgg
    10141 acgatccgtc tggtcgcgcg gttggcgttc accagtcggc atataaataa gacgtgtaat
    10201 attgccccgg agaaatccgg ggcaatttca gttactgggg cgcttgttga ttctgatatg
    10261 cagtaaggaa atgttccggg tttgccacat cgctgtttga caactcatta ctgtaggcca
    10321 gtatttcata gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtatgac
    10381 aaaagaatgg ctgtagggtg gggaaaaaac acaaagtaaa caataattga cgaatatagc
    10441 gccatgctgt tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag
    10501 gttgacgctg caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga
    10561 aaaaaaagat ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca
    10621 caggttcgaa tcccgtcgta gccaccatct tttttgcggg agtggcgaaa ttggtagacg
    10681 caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca
    10741 ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat
    10801 accggcattc cctggttcga atccaggtac cccagccata ttcttcgaat aagcggttca
    10861 ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    10921 ctggttcgaa tccaggtacc ccagccatcg aagaaacaat ctggctacgt agctcagttg
    10981 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccaa
    11041 attctgaatg tgtcgaatat gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca
    11101 agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc
    11161 caatttattc aagacgctta ccttgtaagt gcacccagtt ggggtatcgc caagcggtaa
    11221 ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa
    11281 aaaagctcgc ctcggcgagc tttttgcttt tctgcgtata ttcaatgccg aatgcgatgt
    11341 tgactcgtct tatccttcaa agtcggatgc gacgctgccg cgtcttatcc gacctacggt
    11401 tggcatgcat ccagcaatgt tgtgaggcta caaccctaac gcatatttca gcgccttacg
    11461 tttcaacacg ccagcacgct ccgccgccat caaaccaaga ttacgcacaa aacgcagcgg
    11521 tggcagatta ttgctgaatc cggcataaaa cagatccata ccactttgca tcataaagtt
    11581 atccgccata cgccgcatct ggtaacgctt gagcacagga taactggccc acgcttcgcc
    11641 gtagctgcgg gcgttcacca gaacgtcaat cagggcatcg acatcacgat aaccaagatt
    11701 caccccctgc cccgccagcg gatggatggt atgcgcggca tcgcccacca gagcaagccc
    11761 tggttgcaca tactgcaacg catggcgacg tatcagcgga aacgcaccag cggcaagcgg
    11821 tgtcacgtaa cccagacgcg acgggaaatg cttcgcgatt tccgcctgta gctgcgccat
    11881 attcatattc tgcaactggc gaatgcgcgc cggagagtca taccacacca gcgatgccca
    11941 gttatcaaac aacggcagaa acgcacgcgg tccgtccggg gtaaattgct gccaggtgct
    12001 gtcgccggga tcgttctcac actgtacgct aatcaacata cacgactgcg catactgcca
    12061 ggcatgaacg ccaattcccg ccatctgtcg cacctgcgaa tttgcgccgt cggcaccaat
    12121 caccagcttc gcgaggatcg tttcaccgcc cttcagttcc agctcctgaa gatcattatg
    12181 gcgatgcagt gcaatcagcg aggttggcac acgtaacgtt actttcggat gcgcttccag
    12241 cgcctgccac aacgcctgtt gcaggacagt gttttccacc atgtagccaa gcaacggtag
    12301 cttaagttca gcggcgtcaa acaccacatg cgccgtttcc cactcccacg tttccagtct
    12361 gcggtaagga tggcaacgca tagcctgtac tgcatcccag acccctaacc ctttaagcaa
    12421 tgataccgaa gccgcactaa tcgccgagat ccgcacgtcc ggctggctgt cagcgacaaa
    12481 cggtgcaggt tgggcatgtt cgataaccat taccgaaaat ccgtgctgag ccagtcccag
    12541 cgccagtgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg gttgatttgt
    12601 catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta cccccaaggg
    12661 ctgataacgc tcatactggt cacaacggca gcaaagcatt acactatgcg ccctgcattc
    12721 ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg
    12781 ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg ccacccacgg
    12841 ctatcaactg accgacgtgg cggaagaagc ggatgtgctg ctgctgaaca cctgctcaat
    12901 ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac tgttaaaaga
    12961 gaagaatcca gacctgatta tcggcgtcgg cggctgcgtg gcatcgcaag aaggcgagca
    13021 cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct
    13081 gccggagatg atcaactccg tgcgcggcga ccgcagcccg gttgtagata tcagcttccc
    13141 ggaaatcgag aagtttgacc gtctgccgga accgcgcgcc gaagggccga ccgcgtttgt
    13201 ctccatcatg gaaggctgca ataaatattg cacctactgc gtagtgcctt acacccgtgg
    13261 tgaagaggta agccgtccgt ccgacgatat tctgtttgag attgcccagc ttgctgctca
    13321 gggcgtgcgt gaagttaacc tgctcggcca gaacgtgaac gcctggcgcg gtgagaacta
    13381 cgacggcacc accggatcgt ttgccgatct gctgcgtctg gttgcggcga tcgacgggat
    13441 cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata tcatcgaagt
    13501 gtaccgcgac acgccggagc tggtgagctt cctgcatctg ccggtacaga gcggttccga
    13561 tcgcattctg aacctgatgg gacgtaccca tacagcgctg gagtacaaag cgatcatccg
    13621 taagctgcgt gcggcgcgtc cggatattca gatcagttct gacttcatcg ttggcttccc
    13681 tggcgaaacc accgaagact tcgagaaaac gatgaagctg attgccgacg tcaatttcga
    13741 catgagctac agctttatct tctccgctcg tccaggtaca ccagccgccg atatggttga
    13801 tgatgttccg gaagaagaga agaagcagcg cctgtatatt ctacaagaac gtatcaatca
    13861 gcaagcaatg gcatggagcc gccggatgct cggtaccacc cagcgtattc tggtagaagg
    13921 tacatcgcgt aagagcatca tggagctttc cggccgtact gaaaataacc gcgtcgtcaa
    13981 cttcgaaggt acgccggata tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta
    14041 cccgaactct ctgcgcggta aagtggtgcg aactgaagat gaaatgggtc tgcgcgtggc
    14101 agaaacaccg gaatcggtga ttgcccgtac ccgcaaagaa aacgaccttg gcgtgggtta
    14161 ttatcagccg taattctcag gcccgccgtt ccggtgggcc ttgttttttc tctctttatc
    14221 cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt ccttataggc
    14281 cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa catagacact
    14341 cgcgaaatca ccctggagcc agcagacaat gcgcgtctgt tgagcctgtg cggcccgttt
    14401 gatgacaaca tcaaacaact cgaacgccgt ctcggcatcg aaatcaatcg ccgcgataac
    14461 cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat tctgcgtagc
    14521 ctgtatgtcg atactgcccc gatgcgcggt cagattcagg atatcgaacc ggaacagatc
    14581 caccttgcga ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt gccggagtac
    14641 ggcaaagcgg tcaatatcaa aaccaaacgc ggcgtaatta agccgcgcac gccaaaccag
    14701 gcgcagtaca tcgccaatat tctcgaccat gacattacct tcggcgttgg cccggcgggt
    14761 acgggtaaaa cctacctggc agtggctgcg gcagttgatg ccctggagcg tcaggaaatt
    14821 cgccgtattc tgctgactcg tccggcagta gaagccggtg agaaactggg cttcctgcct
    14881 ggcgatttaa gccagaaagt agacccgtat ctgcgcccgc tgtacgacgc gctgtttgaa
    14941 atgctgggct ttgagaaagt cgagaaactg attgagcgca acgttattga agtcgcaccg
    15001 ctggcctata tgcgtggtcg tacgctgaac gacgcgttta tcattcttga tgagagccag
    15061 aacaccacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg
    15121 gttatcaccg gcgacgtcac acagatcgac ctgccgcgta ataccaagtc tggtttgcgt
    15181 catgccatcg aagtactggc tgatgtcgaa gagatcagct ttaacttctt ccacagcgaa
    15241 gacgtggttc gtcacccggt ggtggcgcgt atcgttaacg cctatgaagc ctgggaagaa
    15301 gccgaacaaa aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga acaggaacaa
    15361 aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc gggttaccgg
    15421 aagagagcca gtttcagaca tggctgaatg cggtgatccc gcagtttcag gaagaatcgg
    15481 aagtgactat tcgcgtggtc gataccgccg aaagccacag tctgaatctg acctatcgcg
    15541 gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtaccgcct ggcatggaaa
    15601 tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc
    15661 aaggcaaacc actggaggcg cactgggcgc atatggtggt gcacggcagt ctgcatttgt
    15721 taggttacga tcacatcgaa gatgacgaag cagaagaaat ggaagccatc gaaacagaga
    15781 ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa tttgccaggc
    15841 atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga ttaactaaca
    15901 tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca cgataagcaa
    15961 caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac cgaaaaaccg
    16021 tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg acgaagatac
    16081 gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg acatcatgat
    16141 cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat gccttgatgt
    16201 catcatcgag tccgcccact cacgtttccc ggtgattagc gaagacaaag atcacattga
    16261 agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg aagccttcag
    16321 catggacaaa gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg tagaccggat
    16381 gctgaaagag tttcgctctc agcgttacca catggcgatc gtcattgacg aattcggtgg
    16441 ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg agattgaaga
    16501 cgagtatgac gaagaagatg atatcgactt ccgtcagctg agtcgtcata cctggaccgt
    16561 gcgcgcactg gcttctattg aagacttcaa cgaagcgttc ggcacccact ttagcgatga
    16621 agaggttgac actatcggtg gtctggtgat gcaggcattt gggcatcttc cggcgcgtgg
    16681 cgaaaccatc gacatcgacg gttaccagtt caaagtggcg atggccgaca gtcggcgtat
    16741 tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccgaagctgg atgaataaaa
    16801 ccgaaactgg atagataact acatggcttt tgcctcatta attgaacgcc agcgcattcg
    16861 cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc cttacgacgt
    16921 ctggcctgcg gcgattattt cgctgatggg gcttcaggcg ttgaccttta accgccgtcc
    16981 actccagtct gccgctattg gcttttgctg gggatttggc ctctttggca gcggtattaa
    17041 ctgggtctat gtcagcatcg cgacctttgg cggaatgcct ggcccggtta acatcttcct
    17101 ggtggtactg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc
    17161 gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccgcccctg ctctctggca
    17221 agtgaccgag tttctgcgcg gttgggtact aacaggcttc ccgtggttac agttcggcta
    17281 tagccagatt gatggcccgt taaaagggct ggcaccgata atgggcgtgg aagccattaa
    17341 cttcctgctg atgatggtta gcggcctgct ggcactggcg ttggtcaaac gcaactggcg
    17401 tccgctggtg gtggccgtcg tgctgtttgc ccttcccttc ccgctgcgtt acatccagtg
    17461 gtttaccccg caaccggaaa aaactattca ggtttcgatg gttcagggcg atattccgca
    17521 atcgctgaaa tgggacggag accagctact taatacgctg aagatatact acaacgcaac
    17581 ggcaccgctg atgggcaaat catcgttgat tatctggccg gagtcggcga taaccgatct
    17641 ggaaattaat cagcaaccgt tcctcaaagc tctggacggt gagttgcgcg ataaagggag
    17701 ctcgctggtc accgggattg tcgacgcgcg tctcaataaa cagaaccgct acgataccta
    17761 caacaccatc atcacactgg gtaaaggcgc accgtacagc tatgaatcag ccgatcgcta
    17821 taacaaaaac catctggtgc cgtttggcga gtttgtccca ctggagtcga tactgcgtcc
    17881 gttagcaccg ttctttgatt tgccgatgtc ttcattcagc cgcggaccat atattcagcc
    17941 accgctgtcg ttgaatggca ttcaacttac tgcggccatt tgctacgaaa tcattctcgg
    18001 cgagcaagtg cgcgataact tccgcccgga taccgactat ctgctgacta tctccaacga
    18061 tgcgtggttt ggtaagtcta ttggtccatg gcagcacttc cagatggcgc gaatgcgtgc
    18121 gctggagctg gcgcgcccac tgttgcgcag caccaacaac ggcattacgg cggtgattgg
    18181 cccgcagggt gagattcagg cgatgatccc gcagttcacc cgcgaggtgt taaccactaa
    18241 cgtgacgccg actaccggac tcacaccgta cgcacgtacc ggcaactggc cgctgtgggt
    18301 gctgacggca ctgtttggtt ttgcagctgt gctgatgagt ctgcgagcac gtaaacatta
    18361 atgtgcctga tgtatccggc atgaacaaaa catactttct cattgagttg tggggcatga
    18421 ttatcatgcc cttttttctt acggttcagg gatacaagcc acatcgccat attggggatg
    18481 ccgaataatt gcctgcggat caagcgagcg cgtcctgtcc atttggatta acctgttctt
    18541 cgccataaca aatatctttc taccatccgc tgaaaaacga aggtgattaa cgtagctggc
    18601 aggataaacg ccaataatct gcccggcatt aatattgagc aggatgacat aaccttgcag
    18661 gccatatgac accaacgcta aatattgacc gtccgggcta ataccctcaa cggaataaac
    18721 gctcctgcta caccattgcg tcataatgct ggcttgttga tcccctacgc ttatacgtga
    18781 aatcccctgg ctatcggcaa aaataatctc gccatttttt ggtcggaatc ccatcgcaga
    18841 aacgatacca cgcgcatcac ctgtgggaaa gtcttctttc tgatgcgtga tggtattgat
    18901 gcgtacaatg tcgcctctga ccataccggg tatcaacaat gttttttcat ccggggaaag
    18961 tgccataacc caggcgcctg gtttaccgct cgtccccgtc ccgtcgtatt tctccagcgg
    19021 gatatccgtc acatgctgat aagtcaccag atcaatggct gacacactat tactggtgcg
    19081 attcatcacc caggcttttg tgccatcacg cgagaggata aggctgtcag gatgggcgcc
    19141 ggtggtaatc gactgaataa tctcccccgt cgccacgtta atgaccgata ctgcattcgc
    19201 ttcaaatgaa gtcgcataaa tcaacttttc atccaggctt aatgccagcg atctgacgcc
    19261 ctgatcttta cagccaccac aatgtttgac tgacttttct ttcgctatgg gcgctgtaaa
    19321 ggtatgcagg attttctttt gtccgaggtc gaaaacaaca atgctgttgg tgttttcaat
    19381 tgcggcatac aacgtattac catcccgact gatcgccata tcatggatat cggcaatctc
    19441 agcgagtaaa tcaccactgg cctgctcacg acttaccgga gcggataatg tttccctgag
    19501 ggcgttgcgc tcctccacta actgctgctt tttttgtcgt cgctcttgtt caatctcctg
    19561 cggataaaac gcttcacgca ggctttgcct gacctgtaaa actgattcat caatctgctg
    19621 ctgcacaaac cagatagtcc cggtcagaag aagagtgacc gcaacaataa tcccgccttc
    19681 cgtaacgagc cgattttgcc caactaaacg cgcactcagc caacccaacg cgccccccgc
    19741 aatcaggccg ccaatgtggc aagcgttatc tattcctgct tgccgggcac catacaacag
    19801 tgtcagcgcc accataccca acagagaaaa tactcgccgc tggtttttat ggtgtgtacc
    19861 cgcacctgaa gaaagctgtg ttgctatcgc agcgcccgct attcccataa ttgccccgga
    19921 tgccccaacg ctgatgacta tctgctcact ttcgcgtaac gcataacagg cgctgatcag
    19981 cccaccaaag atgccggaga ataaccagat aatcaggagt cgccatttcc ccagaatacg
    20041 ttccaccgct ccccccacca ccaggagtgc aaacatattc atcagcaaat gagaaacact
    20101 gctgtgcaga aagacactgc tcaataagcg ccaggattca cccgacaacg tgagagaagc
    20161 aacgttagca ccgagatgga taagtgcgga ttgatcgact tcttcataac tcatccattg
    20221 tgcgaggtaa caaaatatcc caacacagag cgcaaaaagg ataagggtta ttgcgggtaa
    20281 ttgaacattt aacggcttaa ccgaagatgc agacataagg tgattcttcc ttgaaaagac
    20341 aggtaaaaag catttttccc gtgtgcagtt atcatcccgg caaacgtccc atgccgaaaa
    20401 gataaccctg tccgaaatat gactatttca gcctaacatt acaatcgtaa ttaattcacc
    20461 tataagtatt ttttggcatg taagttgctt tatttactgt gtaagcaatg aaatcagccg
    20521 cgacgcaacc gggttgcacc accacaaatc agcggtagca ccaaaaaggt gcgatttaag
    20581 aattttgctt cggaatggtg cggcgcatct cgcaaaaaca aacaataaaa cagcaatatc
    20641 tttacattta gccaaactaa atgttaccta acaagcacaa cactgcacaa taaagttgca
    20701 gatgataaca acacaaacac tcacaacggg tatccatgcg ttcttaacgc agaagataaa
    20761 ggagttggat atgcaattac gtaaacctgc cacagcaatc ctggccctgg cgctttccgc
    20821 aggactggca caggcagatg acgccgcccc ggcagcaggc agtacgctgg acaaaatcgc
    20881 caaaaacggc gtgattgtcg tcggtcaccg tgaatcttca gtacctttct cttattacga
    20941 caatcagcaa aaagtggtgg gctactcgca ggattactcc aacgccattg ttgaagcagt
    21001 gaaaaagaaa ctcaacaaac cagacttaca ggtgaaattg attccgatca cctcacaaaa
    21061 ccgtattcca ctgctgcaaa acggcacttt cgattttgaa tgcggttcta ccaccaacaa
    21121 tgccgaacgc caaaaacagg cggctttctc tgacactatt ttcgtggtcg gtacgcgcct
    21181 gctgaccaaa aagggcggcg atatcaaaga ttttgccgac ctgaaaggca aagccgtagt
    21241 cgtcacttcc ggcaccacct ctgaagtttt gctcaacaaa ctgaatgaag agcaaaaaat
    21301 gaatatgcgc atcatcagcg ccaaagatca cggtgactct ttccgtaccc tggaaagcgg
    21361 tcgtgccgtt gcctttatga tggatgatgc tctgctggcc ggtgaacgtg cgaaagcgaa
    21421 gaaaccagac aactgggaaa tcgtcggcaa gccgcagtct caggaagcct atggttgcat
    21481 gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat gataccatcg ctcaggtgca
    21541 gacctccggt gaagcggaaa aatggtttga taagtggttc aaaaatccaa ttccgccgaa
    21601 aaacctgaac atgaatttcg aactgtcaga cgaaatgaaa gcactgttca aagaaccgaa
    21661 tgacaaggca ctgaactaat tacaagaacc aggggcggaa attccagccc tctcgattgt
    21721 tacgtagcac ggacagacta tacgcctggt ggtcgttccc catcgggcct gaaaaccgca
    21781 atacgctggg taacaatctt cgagggtagc agttaacgct gctaccctct tttttctgga
    21841 gtagatttat gtctatagac tggaactggg gtattttttt acaacaagcc ccgttcggca
    21901 acaccaccta tcttggttgg atctggagtg gttttcaggt gacgatcgct ttgtcgatct
    21961 gcgcctggat tatcgctttc ctcgtcggtt catttttcgg cattttacgt accgttccaa
    22021 accgttttct ctccggtctg ggtacgttgt atgtcgaact gttccgcaac gtgccgttga
    22081 ttgtgcaatt ctttacctgg tacctggtga tcccggagct gctgccagag aaaatcggca
    22141 tgtggtttaa ggcagagctg gatcccaata ttcagttttt cctttcatcc atgctctgcc
    22201 tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg cgccgctatt cagtcgctgc
    22261 cgcgtgggca aaaaaatgcc gcgctggcga tgggtctgac gctaccgcag gcttaccgct
    22321 acgtactgct gcctaatgct tatcgcgtta tcgtcccgcc gatgacctca gagatgatga
    22381 acctggtgaa aaactccgcc atcgcctcga ctatcggtct ggtggatatg gcggcgcagg
    22441 cgggtaaatt gctcgattac tcggcccacg cctgggagtc gtttaccgcc atcacgctgg
    22501 cgtatgtatt gattaacgct tttatcatgc tggtgatgac gctggttgaa cgtaaagttc
    22561 gtctgcccgg caacatgggg ggcaaataat gtacgagttt gactggagtt ccattgttcc
    22621 ttccctgcca tatctgctcg acgggctggt gatcacgctg aaaatcaccg tcacggcagt
    22681 ggtgattggt attttgtggg gcaccatgct ggcggtgatg cgcttgtcca gctttgcgcc
    22741 tgttgcgtgg tttgccaaag cctatgttaa cgtattccgc tcgattcctt tagtcatggt
    22801 tttgctgtgg ttttacctga ttgtgccggg atttctgcaa aacgtgctgg gattatcgcc
    22861 aaaaaatgat atccgtttaa tttcggcaat ggtggcgttt tcgatgtttg aagcggccta
    22921 ctattcagag attatccgtg ccggtattca aagtatttcc cgtggtcagt cgagcgccgc
    22981 gctggcgttg gggatgactc actggcagtc gatgaaactg attattctgc cgcaggcttt
    23041 ccgcgcgatg gtgccgctgc tgctcactca gggcatcgta ctgttccagg atacctcgct
    23101 ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgct tcaaccattg gtgagcgtga
    23161 tggtacgcag gttgagatga tcctgtttgc cggatttgtt tatttcgtta ttagccttag
    23221 cgcgtcgttg ttggtcagct acttgaaaag aaggacagca taatgattac cctgaaaaat
    23281 gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact gctcaaccga agtgaaaaaa
    23341 ggcgaagtgg tggtggtttg cggcccgtct ggttccggca aatcaacgct gattaaaacc
    23401 gtcaacggcc tcgaaccggt gcagcaaggt gaaatcaccg tcgatggcat cgtggttaac
    23461 gacaagaaaa ccgatctggc aaagctgcgt tcccgcgtcg ggatggtatt ccagcatttc
    23521 gagctgttcc ctcatctgtc gattatcgaa aacctgaccc tggcgcaggt gaaagtgctt
    23581 aaacgcgata aagcgccggc tcgtgaaaaa gcattgaaat tgctggagcg cgtcgggctt
    23641 tccgcacatg ccaataagtt tccggcgcag ctttccggcg gtcagcagca gcgtgtggcg
    23701 atcgctcgcg cattgtgtat ggatcctatt gccatgctgt tcgacgaacc gacatcggcg
    23761 ctggatccgg agatgatcaa cgaagtgctg gacgtgatgg tggaactggc gaacgaagga
    23821 atgaccatga tggtggtgac ccacgaaatg ggctttgccc gtaaagtggc gaatcgggtg
    23881 atctttatgg acgagggtaa aattgtcgaa gactcgccga aagacgcttt cttcgatgat
    23941 ccgaaatcgg accgcgcaaa agacttcctc gcgaaaatcc tgcattaatc actctggcgc
    24001 gcatacccaa gtgcgcgcca tctcacgctt tatcgtttcg ctcctcgtat ctgttacatc
    24061 ctagcggtac ccttgtcgca aagaagcaca caacaaggag caacaatggc actgccaatt
    24121 ctgttagatt gcgacccagg tcatgacgac gctatcgcaa tagttcttgc cctcgcctca
    24181 ccagagcttg atgtcaaagc aattacgtct tccgccggaa accagacacc agaaaaaacc
    24241 ttacgcaatg ttctgcgtat gctgaccttg cttaatcgca ccgatattcc ggtagcaggc
    24301 ggcgcggtaa aaccgttaat gcgtaatttg attattgcgg acaatgtgca cggcgaaagc
    24361 ggtctcgacg gcccggcatt accggaaccg acgttcgcac cgcaaaactg tacggcggtg
    24421 gagctaatgg cgaaaacgct gtgtgaaagt gaggaaccta tcaccattgt ttcaaccgga
    24481 ccgcaaacta acgttgcctt gctgctcaat agccacccgg aactgcatag caaaattgcc
    24541 cgtatcgtga ttatgggcgg cgcaatgggg cttggtaact ggacgcctgc ggctgaattt
    24601 aatatttacg ttgacccgga agcggcagaa attgtcttcc agtcaggaat tccggtggtg
    24661 atggccggtc tggatgttac tcacaaagca caaatccacg ttgaagacac tgagcgtttc
    24721 cgcgcgattg gcaaccctgt ttcaaccatt gttgccgaac tgctggattt cttcctcgaa
    24781 tatcataaag acgaaaaatg gggctttgtc ggcgcaccac tgcatgaccc atgcaccatc
    24841 gcctggctgt tgaaaccgga gttgtttacc actgttgagc gctgggttgg cgtggaaaca
    24901 caagggaaat atacccaggg tatgacggtt gttgattatt attatctgac tggcaataaa
    24961 ccgaatgcca ccgtaatggt cgatgttgat cgtcagggct ttgttgattt actggctgat
    25021 cgtctgaaat tttacgctta atcactcgcc ggatgacatc acgccatccg gcaccactca
    25081 tttcattacg gctcgaacgg acgtctctgg aactgatcat gaccacattt cgggcatagc
    25141 gtcagcactt ccggtgtata gatcgggaga tggaagtgac atttctcgca gaccagattt
    25201 cccagcccta ccacttctcc gctgtgataa accccgtgat gattgaggtc ctggaaaact
    25261 tcgcgccatt caagctgcgt tttatcggtg atgtctgcca gctcctgcca caagctttct
    25321 ttaatcaccc gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg
    25381 gcgaactctt ccaggtcacg tctgacagct cgcgtcagct catcgacctc ggttcgcgtt
    25441 aactcccctg tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt
    25501 tcgccattgc gcaggcgttc gctcagtgac gcgaccagtt cacggtaata ttgagcaacc
    25561 ttgttcatcg tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc
    25621 cttgctttgc taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca
    25681 tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg
    25741 atctgaaaaa ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg
    25801 ccatgcaaga gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg
    25861 agaagcgcac atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta
    25921 tgcttcccta tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg
    25981 acgtgatcgc ccgctaccag cgtatgctgg gcaaaaacgt cctgcagccg atcggctggg
    26041 acgcgtttgg tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgca ccggcaccgt
    26101 ggacgtacga caacatcgcg tacatgaaaa accagctcaa aatgctgggc tttggttatg
    26161 actggagccg cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct
    26221 tcaccgagct gtataaaaaa ggcctggtat ataagaagac ttctgcagtc aactggtgcc
    26281 cgaacgacca gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg
    26341 ataccaaagt tgagcgtaaa gagatcccgc aatggtttat caaaatcacc gcttacgctg
    26401 acgagctgct caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc
    26461 agcgtaactg gatcggtcgt tccgaaggcg tggaaatcac cttcaacgtt aacgactatg
    26521 acaacacgct gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg
    26581 cggtggctgc gggtcatccg ctggcacaga aagcggcgga aaataatcct gaactggccg
    26641 cctttattga cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga
    26701 aaaaaggcgt cgatactggc tttaaagcgg ttcacccatt aacgggcgaa gaaattcccg
    26761 tttgggcagc aaacttcgta ttgatggaat acggcacggg cgcagttatg gcggttccgg
    26821 gtcacgacca gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccggtta
    26881 tcctggcagc tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg
    26941 tgctgttcaa ctctggcgag ttcaacggtc ttgaccatga agcggccttc aacgccatcg
    27001 ccgataaact gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact
    27061 ggggtgtttc tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg
    27121 gtaccgtaat gccgaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga
    27181 tggacggcat taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg
    27241 gtatgccagc gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact
    27301 atgcgcgcta cacttgcccg gagtacaaag aaggtatgct ggattccaaa gcggctaact
    27361 actggctgcc ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct
    27421 acttccgctt cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag
    27481 caaaacagtt gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa
    27541 atggcgaacg taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc
    27601 gtatcgtgaa agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa
    27661 tgtccaagtc gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg
    27721 acaccgttcg tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg
    27781 aatccggcgt ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtttacgagc
    27841 acacagcgaa aggtgatgtt gcggcactga acgttgatgc gctgactgaa gatcagaaag
    27901 cgctgcgtcg cgatgtgcat aaaacgatcg ctaaagtgac cgatgatatc ggccgtcgtc
    27961 agaccttcaa caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac
    28021 cgaccgatgg cgagcaggac cgcgccctga tgcaggaagc actgctggcc gttgtccgta
    28081 tgcttaaccc gttcaccccg cacatctgct tcacgctgtg gcaggaactg aaaggcgaag
    28141 gcgatatcga caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca
    28201 cgctggtcgt ggtgcaggtt aacggtaaag tccgtgcaaa aatcaccgtt ccggtggacg
    28261 caacggaaga acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg
    28321 atggcgttac cgtgcgtaaa gtgatttacg tacccggtaa actcctcaat ctggtcgttg
    28381 gctaagcgcg ggaggaagcg tgcgatatct ggcaacattg ttgttatctc tggcggtgtt
    28441 aatcaccgcc gggtgtggct ggcatctgcg tgataccacg caggttcctt ccactatgaa
    28501 ggtcatgatc ctggactcag gcgatccgaa cgggccatta agccgtgcgg tgcgtaacca
    28561 gttacgtcta aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc
    28621 cctgcgtttg ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca
    28681 aacagcagag tatcagatga tcatgacggt caatgcgacc gtgttgatcc ccggccgtga
    28741 catctacccg attagcgcca aagtcttccg ttcgttcttc gataacccgc aaatggcgtt
    28801 agcgaaagat aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca
    28861 gctgattcgt aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac
    28921 gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc tctaccacgc tgggtaactg
    28981 atgattcggt tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg
    29041 tatcttttac ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tattcgtcag
    29101 gtagctgcgg tacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac
    29161 tggaatgcga tcttttcgtt atgccaggcc atgagtctgt ttgccagtcg acaaacgcta
    29221 ttgctgttgt taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc
    29281 accggacttc tgcatgacga cctgctgttg atcgtccgcg gcaataaatt aagcaaagcg
    29341 caagaaaatg ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag
    29401 acaccggagc aggctcagct tccccgctgg gttgctgtgc gcgcaaaaca gctcaactta
    29461 gagctggatg acgcggcgaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg
    29521 ctggctcagg cactggagcg tttatcgcta ctctggccag acggcaaact gacattacct
    29581 cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct
    29641 ttgttgatgg gaaaaagtaa gcgcgcgttg catattcttc agcaactgcg tctggaaggc
    29701 agcgaaccgg ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg
    29761 aaacgccagt ctgcccatat gccactgcgt gcgttgtttg ataagcatcg ggtatggcag
    29821 aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa gccatccgca gttacgtcag
    29881 gccgtgcaac tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg
    29941 tgggcagagc ttgaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt
    30001 atcgacggtt gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca
    30061 ctatggtcat cttaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac
    30121 aatcatccct aataatgttc ctccgcatcg tccccagccg gaagcaaaca gcatgcagcg
    30181 taaacacatg cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga
    30241 gctaaagcgc aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggagca
    30301 aggaccggac gtgccgctgg cgtttattat tggtcaggat tcattgctga cctttccgac
    30361 ctggtacgaa tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtcctgg
    30421 ttacccactt gaaatggcac aaccacaata tcagcaatgg ctggaggatc atttgacaca
    30481 taacccggaa gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg
    30541 gtttaacatc tcggcgacca tcatccgcga acgtttgcaa aatggtgaat cgtgtgagga
    30601 tttattaccg gaaccggtac tgacttacat taaccaacaa ggcttgtatc gctgatacct
    30661 gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc atggtgaaac gcaagcgaat
    30721 gtcgatggtc tgtacagcgg tcatgcgccc acacccctga ccgcgcgcgg tattgagcaa
    30781 gcgcaaaatc tgcatacgct gctacatgat gtttcctttg acctggtttt atgcagtgaa
    30841 ctggaacggg cacagcatac cgcgcgactg gttcttagtg accgccagca ccccgttcat
    30901 atcatccctg aactcaatga aatgtttttt ggcgactggg agatgcgaca tcatcgtgac
    30961 ctcatgcaag aagatgccga aaactatagc gcgtggtgca atgactggca gcatgctatc
    31021 cccacgaacg gtgaaggatt tcaggcattt tcgcaacgtg tggaacgatt tatcgcaagg
    31081 cttagtgaat atcagcacta tcagaatatt ttagtcgtca gccatcaggg cgtactgagt
    31141 ctgttaattg ctcgtttaat tggcatgcct gccgagtcta tgtggcattt ccgcgttgac
    31201 caggggtgtt ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat
    31261 agccgtgcca tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa
    31321 cgaccattga cagccccggg caggctgata ttctccgcag ccagactttt tccgccagac
    31381 acgactttgt agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat
    31441 gatagcccac tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag
    31501 gtatactgac agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa
    31561 agcactccag gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc
    31621 cttagacgtt cagggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc
    31681 cagccgccat gttatgtcca ttgctgatca cgttgtgcag gagtctcgcg cagcgggcct
    31741 gttaccgctc ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga
    31801 tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg
    31861 gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca
    31921 aaccggtttt accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga
    31981 aattccggcc ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg
    32041 tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa
    32101 gccctgggat acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga
    32161 cgtcagtcta ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga
    32221 gcagagctgg tcgctgtcgg cacttaccct tccccatccg ctggttcgcg tgctggtcgc
    32281 agagagtctg taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata
    32341 agtgagcttt gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac
    32401 ggcagagtcc gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct
    32461 gaccggcgtg cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca
    32521 gacccgctct aatgaaaacc gcattaagct ggtgcctatc gcacccagcc gcggcattat
    32581 ctacgaccgt aacggtatcc ctctggctct caaccgcact atctaccaga tagaaatgat
    32641 gccggagaaa gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct
    32701 gaccgatgat gatattgcgg cattccgaaa agagcgcgca cgttcacacc gtttcacctc
    32761 tattccggtg aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaaccagta
    32821 ccgttttccg ggtgttgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc
    32881 gttgacccac gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa
    32941 taatgacggc aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga
    33001 acgttactat gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa
    33061 ccgtgggcgt gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta
    33121 cctgacgctg gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc
    33181 agctgtggta gtcaccgatc cgcgtacagg tggggtgttg gcgctggttt ccacgcctag
    33241 ttatgaccca aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgctgaa
    33301 cgacccgaat acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac
    33361 agttaaaccc tatgttgcgg tttcggcatt gagtgccggg gtgatcacgc gcaatacgac
    33421 gctgtttgac cccggctggt ggcaactgcc agggtcggaa aaacgttatc gtgactggaa
    33481 aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt
    33541 cttctatcag gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt
    33601 cggttatggt cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac
    33661 ccgcgaatgg aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt
    33721 tggtatcggt cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat
    33781 cctgattaat gacggtatcg tgaaggttcc gcatttgctg atgagcaccg ccgaagacgg
    33841 caaacaggtg ccatgggtac aaccgcatga accgcccgtc ggcgatattc attccggtta
    33901 ctgggagctg gcgaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca
    33961 taaatatttt gccagcgcac cgtacaaaat tgcggcgaaa tccggtaccg ctcaggtctt
    34021 cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca
    34081 caaactgatg accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct
    34141 ggagaacggt ggcgcgggtc cggcggttgg tacgctgatg cgccagatcc tcgaccacat
    34201 tatgctgggt gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga
    34261 ggaccattaa tcatgacaga taatccgaat aaaaaaacat tctgggataa agtccatctc
    34321 gatcccacaa tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg
    34381 agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa tcggccagat tgcgatgggt
    34441 ctggtcatca tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc
    34501 tatctctata tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct
    34561 aaaggcgctc aacgctggct ggacctcggt attgttcgtt ttcaaccgtc ggaaattgcc
    34621 aagatagccg taccgttgat ggttgcacgc tttatcaacc gcgacgtttg cccaccttca
    34681 ttgaagaata cagctatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca
    34741 cagcctgact tgggaacatc aatcctcgtt gcgctttccg gcctgtttgt actgttcctc
    34801 tctggcctta gctggcgtct gattggcgtc gcagtagtgc tggtagcggc gttcattccg
    34861 attctgtggt tctttctgat gcatgattac cagcgccagc gcgtaatgat gctcctggac
    34921 ccggaatcag acccactcgg cgcaggctat cacattattc agtctaaaat tgctattggc
    34981 tccggcggat tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc
    35041 cccgaacgcc ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc
    35101 attttgattc tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc
    35161 agagcgcaaa ccacctttgg tcgcgtcatg gctggcggct taatgctgat attattcgtt
    35221 tatgtcttcg taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc
    35281 ccactggtca gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta
    35341 atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg
    35401 taagcagtgg ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga
    35461 tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga
    35521 aattagcggg gcagacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca
    35581 gcgcgacggg aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact
    35641 ggcggcaatc tatgatgccg aacccggcag taacctgacg gcctctggcg aagctttcga
    35701 tccgacacag ttgacggcgg cccatccaac gcttccaatc cccagctacg ccagaatcac
    35761 taacctggct aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc tttatggcaa
    35821 cgaccgcgtg atttcgcttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac
    35881 caaagttcgt atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat
    35941 ggcttgtacc acagtcgcca aacagactta cgccctgcca gcacctcccg atttaagcgg
    36001 tggcgcggga acaagttcag tgcctgtccc gcagggtgac attcttccgg tcagtaattc
    36061 gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc agcagcggtt tcctcggcgc
    36121 accaacgacc ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc
    36181 cgttgttaca gctccgtcga caacgcctgc aacctcacct gcaatggtga caccgcaagc
    36241 cgcctcgcaa agcgccagcg gcaactttat ggtgcaagtc ggggccgtta gcgatcaggc
    36301 tcgtgcgcaa cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac
    36361 tcaaaatggc gcggtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag
    36421 taccttgcag caacgtttgc aaaccgaagc ccaattacag tcatttatta ccaccgcgca
    36481 gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc
    36541 ggaaagtcag atgcctgccg gtagtgcatt tgctatagta gggcactttt tttaattcca
    36601 tcacggatgt cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc
    36661 tggcgctcac cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga
    36721 atatcaaaac catgatcccg ggtgtaccgc agatcgacgc ggagtcctac atcctgattg
    36781 actataactc cggcaaagta ctcgccgaac agaacgctga tgtccgccgc gatcctgcca
    36841 gcctgaccaa aatgatgacc agttacgtta tcggccaggc aatgaaagcc ggtaaattta
    36901 aagaaactga tttagtcact atcggcaacg acgcatgggc caccggtaac ccggtgttta
    36961 aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc
    37021 gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc catggccgat tttgccgctg
    37081 gtagccagga cgcttttgtt ggcttgatga acagctacgt taacgccctg ggcctgaaaa
    37141 atacccactt ccagacggta cacggtctgg atgctgatgg tcagtacagc tccgcgcgag
    37201 atatggcgct gatcggccag gcgttgatcc gtgacgtacc gaatgaatac tcgatctata
    37261 aagaaaaaga atttacgttt aacggtattc gccagctgaa ccgtaacggc ctgttatggg
    37321 ataacagcct gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca ggttacaacc
    37381 ttgttgcttc tgcgactgaa ggccagatgc gcttgatctc tgcggtgatg ggcggacgta
    37441 cttttaaagg ccgtgaagcc gaaagtaaaa aattgctaac ctggggcttc cgtttcttcg
    37501 aaaccgttaa tcctctgaaa gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg
    37561 attctgatcg cgcttcgtta ggggttgata aagacgtgta cctgaccatt ccgcgtggtc
    37621 gcatgaaaga tctgaaagcc agctatgtgc tgaacagcag tgaattgcat gcgccgctgc
    37681 aaaagaatca ggtcgtcgga actatcaact tccagcttga tggcaaaacg atcgaacaac
    37741 gcccgctggt tgtgctgcaa gaaatcccgg aaggtaactt cttcggcaaa atcattgatt
    37801 acattaaatt aatgttccat cactggtttg gttaaaaatt gaacacttga aagtgtaatt
    37861 tccgtcccca tatactaagc atcagtaaaa aactcccgcc ttctggcggg agttgctatt
    37921 taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct
    37981 actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt
    38041 gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa
    38101 ggcaactacc actcggtatc tatcactatc aacgccactc atatcgagca ggttgaaaca
    38161 ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg
    38221 ttacccggct cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact
    38281 tttactcatt ctccacggag atgccgtttt gtatcaggat aaaattcttg tccgccagct
    38341 cggtcttcag ccttacgagc caatctccca ggctatgcat gaattcaccg atacccgcga
    38401 tgatagtacc cttgatgaaa tctggctggt cgagcactat ccggtattca cccaagggca
    38461 ggcaggaaag gcggagcaca ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg
    38521 cggtgggcag gtgacttatc acgggccggg gcaacaggtg atgtatgtgt tgcttaacct
    38581 gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa
    38641 taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt
    38701 cggggaaaag aaaatttgct cactgggttt acgtattcga cgcggttgtt cattccacgg
    38761 tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta
    38821 tgcaggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat
    38881 tgcaccacgt ttactggaaa atattttagc gctactaaac aatcctgact tcgaatatat
    38941 taccgcttaa ttccatacat caatggccca atttacactg ggtcattact cataatacct
    39001 gctaatgctt tacatctcct tcgcgtttgt atattttctt tatcacttgc agaaatgcga
    39061 tgctacgcct tgctccgtag cccacctaat tgattaaccc attgaaataa ttgaccatgg
    39121 tgagcaaaag cgttccgtct caattagaca taagattatt gtaatttttt t
//
LOCUS       DAZGAP010000016        39103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGAP010000016 DAZGAP010000000
VERSION     DAZGAP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.016"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..800)
                     /locus_tag="ACHLAM_000748"
     CDS             complement(<1..800)
                     /locus_tag="ACHLAM_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO7852323.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDVEYIFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITELIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAIHEIERYLYATFKYELILEEKLYLS"
     gene            complement(1058..2311)
                     /gene="amiC"
                     /locus_tag="ACHLAM_000749"
     CDS             complement(1058..2311)
                     /gene="amiC"
                     /locus_tag="ACHLAM_000749"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO7852324.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2543..3874
                     /gene="argA"
                     /locus_tag="ACHLAM_000750"
     CDS             2543..3874
                     /gene="argA"
                     /locus_tag="ACHLAM_000750"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO7852325.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(4112..5938)
                     /gene="recD"
                     /locus_tag="ACHLAM_000751"
     CDS             complement(4112..5938)
                     /gene="recD"
                     /locus_tag="ACHLAM_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO7852326.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5938..9480)
                     /gene="recB"
                     /locus_tag="ACHLAM_000752"
     CDS             complement(5938..9480)
                     /gene="recB"
                     /locus_tag="ACHLAM_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO7852327.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKLLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALMLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(9473..12361)
                     /gene="ptrA"
                     /locus_tag="ACHLAM_000753"
     CDS             complement(9473..12361)
                     /gene="ptrA"
                     /locus_tag="ACHLAM_000753"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO7852328.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12537..15905)
                     /gene="recC"
                     /locus_tag="ACHLAM_000754"
     CDS             complement(12537..15905)
                     /gene="recC"
                     /locus_tag="ACHLAM_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO7852329.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEIGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFRMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDADRLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIARRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15918..16241)
                     /gene="ppdC"
                     /locus_tag="ACHLAM_000755"
     CDS             complement(15918..16241)
                     /gene="ppdC"
                     /locus_tag="ACHLAM_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO7852330.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(16226..16633)
                     /gene="ygdB"
                     /locus_tag="ACHLAM_000756"
     CDS             complement(16226..16633)
                     /gene="ygdB"
                     /locus_tag="ACHLAM_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO7852331.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16630..17193)
                     /gene="ppdB"
                     /locus_tag="ACHLAM_000757"
     CDS             complement(16630..17193)
                     /gene="ppdB"
                     /locus_tag="ACHLAM_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7852332.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(17184..17654)
                     /gene="ppdA"
                     /locus_tag="ACHLAM_000758"
     CDS             complement(17184..17654)
                     /gene="ppdA"
                     /locus_tag="ACHLAM_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7852333.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17839..18633)
                     /gene="thyA"
                     /locus_tag="ACHLAM_000759"
     CDS             complement(17839..18633)
                     /gene="thyA"
                     /locus_tag="ACHLAM_000759"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO7852334.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18640..19515)
                     /gene="lgt"
                     /locus_tag="ACHLAM_000760"
     CDS             complement(18640..19515)
                     /gene="lgt"
                     /locus_tag="ACHLAM_000760"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO7852335.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19666..21912)
                     /gene="ptsP"
                     /locus_tag="ACHLAM_000761"
     CDS             complement(19666..21912)
                     /gene="ptsP"
                     /locus_tag="ACHLAM_000761"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7852336.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLARRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21925..22455)
                     /gene="rppH"
                     /locus_tag="ACHLAM_000762"
     CDS             complement(21925..22455)
                     /gene="rppH"
                     /locus_tag="ACHLAM_000762"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO7852337.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22808..22954)
                     /gene="ygdT"
                     /locus_tag="ACHLAM_000763"
     CDS             complement(22808..22954)
                     /gene="ygdT"
                     /locus_tag="ACHLAM_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO7852338.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            23140..23829
                     /gene="mutH"
                     /locus_tag="ACHLAM_000764"
     CDS             23140..23829
                     /gene="mutH"
                     /locus_tag="ACHLAM_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO7852339.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23898..24611
                     /gene="ygdQ"
                     /locus_tag="ACHLAM_000765"
     CDS             23898..24611
                     /gene="ygdQ"
                     /locus_tag="ACHLAM_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7852340.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24749..24967
                     /gene="ygdR"
                     /locus_tag="ACHLAM_000766"
     CDS             24749..24967
                     /gene="ygdR"
                     /locus_tag="ACHLAM_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO7852341.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            25075..26115
                     /gene="tas"
                     /locus_tag="ACHLAM_000767"
     CDS             25075..26115
                     /gene="tas"
                     /locus_tag="ACHLAM_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO7852342.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(26147..27340)
                     /gene="lplT"
                     /locus_tag="ACHLAM_000768"
     CDS             complement(26147..27340)
                     /gene="lplT"
                     /locus_tag="ACHLAM_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO7852343.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRY"
     gene            complement(27333..29492)
                     /gene="aas"
                     /locus_tag="ACHLAM_000769"
     CDS             complement(27333..29492)
                     /gene="aas"
                     /locus_tag="ACHLAM_000769"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO7852344.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            30078..31109
                     /gene="galR"
                     /locus_tag="ACHLAM_000770"
     CDS             30078..31109
                     /gene="galR"
                     /locus_tag="ACHLAM_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO7852345.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(31116..32378)
                     /gene="lysA"
                     /locus_tag="ACHLAM_000771"
     CDS             complement(31116..32378)
                     /gene="lysA"
                     /locus_tag="ACHLAM_000771"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO7852346.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32500..33435
                     /gene="lysR"
                     /locus_tag="ACHLAM_000772"
     CDS             32500..33435
                     /gene="lysR"
                     /locus_tag="ACHLAM_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO7852347.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33422..34114)
                     /gene="ygeA"
                     /locus_tag="ACHLAM_000773"
     CDS             complement(33422..34114)
                     /gene="ygeA"
                     /locus_tag="ACHLAM_000773"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO7852348.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADKRAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(34243..35661)
                     /gene="araE"
                     /locus_tag="ACHLAM_000774"
     CDS             complement(34243..35661)
                     /gene="araE"
                     /locus_tag="ACHLAM_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO7852349.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35976..36737)
                     /gene="kduD"
                     /locus_tag="ACHLAM_000775"
     CDS             complement(35976..36737)
                     /gene="kduD"
                     /locus_tag="ACHLAM_000775"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO7852350.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36767..37603)
                     /gene="kduI"
                     /locus_tag="ACHLAM_000776"
     CDS             complement(36767..37603)
                     /gene="kduI"
                     /locus_tag="ACHLAM_000776"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO7852351.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGIINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37890..39071)
                     /gene="yqeF"
                     /locus_tag="ACHLAM_000777"
     CDS             complement(37890..39071)
                     /gene="yqeF"
                     /locus_tag="ACHLAM_000777"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO7852352.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTDAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT         9744 a        10610 c         9537 g         9212 t
ORIGIN      
        1 atagagagat atagtttttc ttctaaaatt aattcatatt taaaagtggc atacagatac
       61 cgttcaattt catgaattgc gcgctgtaac aggatgtcct tttcaagaaa ctgttcatag
      121 atatcaccaa tatctttctg ttttattttt tcattgcata acacgcgact tgaaaaatat
      181 ttaaggtgca tcataaatct ggaatagtta aatgaacttc tgtccacctt tctgttataa
      241 acaacttcaa taagctccgt gattatttgc gacaactcaa taatccgtac tgattcataa
      301 tcaactgaat tttcataatt atttaaaata tgcaatgcta taaaaccaat ctcatcatca
      361 ggaagttgaa cgctgaattg ctcattaatg atagttaatg catcgcgact tatcgcatac
      421 tctgctttgt acaacgcttt gatttcatca aggaaaacat tattaatttg tatacctgaa
      481 actactcggg atattgcatt cgaaatgtga tccgcaagaa tcaatagtag cgatggattg
      541 agattttttt cgagattttt ctccgcatat tgcactattt tttcggcaac aaaaatatac
      601 tcaacatcca catgttcaat caatttgtag agtttatttt tttgttcgtt tctgacataa
      661 aaaatccggt tagccggatg ttcagggacc gacattccat attttttgtt gtaaccgacg
      721 ccaggtccag aaatgataac ctcctgtcca ttcatcgatg cctgtacaca attattgttc
      781 atgacttttt cgatgatcat tccatgactc caaaaaaaaa ggcaaggcac gtcagaagta
      841 ataattactt caaacattgc cttgcctgat ttaacagtta caagatgatt aactaatgcg
      901 tatacattac acggaattgt gcttaattaa agagatggtc ggcttatttt tcaagccgac
      961 attcaaatat tcgacatcag tcacaaaacc agcatctaat attcatgaaa atgttatcta
     1021 ttcaacgaaa tataaattca ttgaataaat aaaaacatca tccccttctc gccagcgtcg
     1081 ccccatcggc aaaatacgct ttaatccccg caagaataga ctccgcaact tcctgctgga
     1141 aagtcgccgt tttcagttta cgttcttcct caacgttact gataaacgcc gtttcgacca
     1201 gaatggaggg aatatctggt gcctttagta cggcaaaccc ggcctgttca acttgatttt
     1261 tatgcagctt gttgatttta ccgagcttat tcagcaccgc tttaccaaac ttcaggctgt
     1321 cggcaatggt cagcgactgc accatatcga acatggtgtg gtcgacatag cggtcaccgc
     1381 ttttgctcac gccaccaatc aagtccgagg cgttctgggt ttgtgccaga tattttgccg
     1441 cagtactggt tgcacctttg gttgagagcg caaacacaga ggaaccgctc ggctgacgac
     1501 tggtaaaggc gtcggcatgg atagagacaa acaggtcagc acgctgtttc tgggcttttg
     1561 ctacgcgcac ttgcaacgga atgaagatgt cttcattgcg cgtcatgtac accttcatat
     1621 tgccctcttt ctcgatcaga gagcgcagac ggcgagctat ttgcaatacc acgtcttttt
     1681 cgcgcgtttt gtatttcccc accgcaccgg agtcttcgcc accgtggcca gggtcaagca
     1741 taatgacaat aggacgatcg cgccctgctt tacccggttg tggaccactt tgtgctggcg
     1801 gcacctgctt ttcgaggtcg cctttgttgt aatcctccag cagcgccagc agcgggtcct
     1861 gcatatcctg tgcattggcc ggatagaggt ccatcaccag acgctcttta aacccggcga
     1921 ccggcgcaag ggcaaacagc tgcggtttta cgttttgctt taattcaaaa accatacgta
     1981 cggtttgcgg gtcaaattgc ccgacgcgcg ccgacttgat gaacgggtcg tcagcgcgga
     2041 tttgcgcagc catccccttg agtaccgagt tcaggtttac atcttcgata tccaccacca
     2101 cgcgttcagg attactcaac gcgaactgct tatatttcag ctgacgattt gattctaccg
     2161 tcacgcgggt gtagctggac gcaggccaga cgcgcaccgc cacgacctgg ctgaccgcag
     2221 ccaggctgac ctgacttacg ctcaatagcc acatggcacc cgcgccttgc agtaaacgac
     2281 gacggctgat tgcagtgttg gatcctgaca tgcctctccc gagcaaaaga aatctaagct
     2341 gtgtaacaag taaacgacca atttgaccgg aaactttagc gaaagacgca taatctgtca
     2401 tctataaaac ggtaaacatt ctttttatat tcacggtatt actgataaaa aactcgctcc
     2461 cgcataaaat ttacacttgc accctgcgaa aaaacagaat aaaaatacaa taatttcgaa
     2521 taatcatgca aagaggtgta ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt
     2581 ccgccattcg gttccctata tcaataccca ccggggaaaa acatttgtca tcatgctcgg
     2641 cggtgaagcc attgagcatg agaatttctc cagtatcgtt aatgatatcg ggttgttgca
     2701 cagcctcggc atccgtctgg tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct
     2761 ggctgcgcat caccacgaac cgctgtatca caagaatata cgtgtgaccg acgccaaaac
     2821 actggaactg gtgaagcagg ctgcgggaac attgcaactg gatattactg ctcgcctgtc
     2881 gatgagtctc aataacacgc cgctacaggg cgcgcatatc aacgtcgtca gtggcaattt
     2941 tattatcgcc cagccgctgg gcgtcgatga cggcgtggat tactgccata gcgggcgtat
     3001 ccggcggatt gatgaagacg cgatccatcg tcaactggac agcggtgcaa tagtgctaat
     3061 ggggccggtc gctgtttcag tcactggcga gagctttaat ctgacctcgg aagagattgc
     3121 cactcaactg gccatcaaac tgaaagctga aaagatgatt ggtttttgct cttcccaggg
     3181 cgtcactaat gacgacggtg atattgtctc cgaacttttc cctaacgaag cgcaagcacg
     3241 ggtagaagcc caggaagaga aaggtgatta caactccggt acggtgcgct ttttgcgtgg
     3301 cgcagtgaaa gcctgccgca gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga
     3361 tggcgcgcta ttgcaagagt tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga
     3421 aagcgccgag cagattcgtc gcgcaacaat caacgatatt ggcggtattc tggagttgat
     3481 tcgcccgctg gagcagcaag gtattctggt acgccgttct cgcgagcagc tagagatgga
     3541 aatcgacaaa ttcaccatta ttcagcgcga taacacgact attgcctgcg ccgcgctcta
     3601 tccgttcccg gaagagaaga ttggggaaat ggcctgtgtg gcagttcacc cggattaccg
     3661 cagctcatca aggggcgagg ttctgctgga acgcattgcc gctcaggcga agcagagcgg
     3721 cttaagcaaa ttgtttgtgc tgaccacgcg cagtattcac tggttccagg aacgtggatt
     3781 taccccggtg gatattgatt tactccccga gagcaaaaag cagttgtaca actaccagcg
     3841 taaatccaaa gtgttgatgg cggatttggg gtaagtggtg gaaatcgcca gatgcgacat
     3901 gcgcaatgcc tggaggcatg ataaacacgc tagcatcgca tcaggtattg acgctcatcg
     3961 tcggatgcgg cgtgaacgcc ttatccgacc tacatcaaat caaaggtgtt gatggcggat
     4021 ttagggtaag cgacggaaat cgccagatgc gcatatgcgc aatgcccgga ggcatgacag
     4081 gacgcgcatg cgtcgcatca ggcaatcacg tttattcccg cgaactaaac aatgccgcca
     4141 gaccactgcg ccgctcagtg cgcgtggcaa ttgccgcact taaaatgcgc tcatcggcat
     4201 acagcgacag acggcgacgc gcgcgggtca ctgcggtgta aaccagctct cgcgttacta
     4261 ccggcgtgcg ttgactcggt aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt
     4321 tatgtaccgt catcgcccac gtcgtttcgt gctctggcag gcgactcggt tgcacagact
     4381 taatattgcc gtccggcatc gcaaaccaaa cgcgcgtccc ctgcccgcga tccagcgcaa
     4441 taccgatatc gccattaaac aacccaagcg cgctgtcatt acgggcaatc atcaccggtc
     4501 ggccttcgta ccaacgagag tgcggattac gatgaatttt gcgcttctgt tgcataaact
     4561 gctcaattcg ctcattcagt ccagccacgc caaacggccc ttcccgcagg gcgcacaaaa
     4621 gctggtactc attgaacgcc tgaatgatta aatccggctc ggcacgcgct tgcagcagat
     4681 ccagataacg cccgtaaccc gcaagagcct cctcaagcat cgcaatatag tcttcgccgc
     4741 tctgtaagag ccgtttttcg atatcagtaa aatcctgctg aaaaacggtt ttcactgccg
     4801 ttttatcacc acggttgatc gccgcagcta actgaccaat gccagaatcg ctgccgaaac
     4861 gatagctttt ttgcagcagg cagagactgt cgcgcaaaga tgccgcttct gtgccagttc
     4921 ctgacggaac gtgagttccc gtcaggcggc tcaactgccc ggcacgctcg gcggtaaagc
     4981 ccgcgttggc ataagcgcag atatcgccca gcacagctcc agcctcaact gaggccagtt
     5041 gatcgcgatc accgaggaag atcactcgcg catgatcggg caacgcatcg atcaggcgcg
     5101 acatcatggg gagatcgatc attgacgctt catccaccac cagcacatca agatgcagcg
     5161 ggttaccggc atgatgacgt aaacgctggc tacccggctg cgcacccagc aatcggtgca
     5221 aagtgctggc atcttccgga atgcgtttct tttgttcatc ggtcagcggt aactggcgca
     5281 aagccttgcc gagagattcg gttaagcgcg cggcagcttt acccgttggt gcagccagac
     5341 ggatacggca gcgttcgccg tctgccattt gaattaacgc tgccagcaac ttcgctacgg
     5401 tggtcgtttt accggtgcca gggccgccgg aaatcaccga gatccgccgc gtcagcgcca
     5461 ctgccgccgc cactttttgc cagttaattt catcgcttac tggaaaaagt ttgtccaggg
     5521 tttgcgccag tagagcttca tcaacctcaa tggcatgatt cacttcgttg aaaaagcgtg
     5581 ccactgtgcg ctcgttacac cacatgcgat tcaaataaag acgatcacca cagaggatca
     5641 tcggcgttgg ttcatctccc cggctgaccg cttgtgaagc cagcaagcat tcttcccaat
     5701 tttgtaactc accgatttcg ctgacgcagg ttgctaacag cggatgtgac gcctcgttat
     5761 tttccagtcg tgagagcggt aaacagacat gcccttcccc ggcgtcgtgg cttaacaggg
     5821 ctgccgcgag ggtgacggca ggatgttcat ctcccgccac ggtcagggca aattgcacat
     5881 ccagcgggcg taactgtttg tgctccacag cttccagtaa ttgcttttgc aatttcatta
     5941 cgcctcctcc agggtgatac cggcaaacat ctcatccatc aggtcaatca acccggcgtt
     6001 gggtcgggtc gcgtagatcc cttgttgcgg atgttcttta tcaacaccac gcaggaacag
     6061 ataaataacg ccgccaaagt ggcgctcata gtcgtaatca gcaatgcgat ggcgcagata
     6121 acggtgcagc gccagggtat aaagctgata ttgcagatca tagcggtgtg cctgcattgc
     6181 cgctgccata gcctgttggg tgtaagcaga actgtcttca cccaaccagt tggatttata
     6241 gtcgagcagg taataacgcc cttcgtggcg gaacaccagg tcaataaagc cttttaacat
     6301 gccgcgtacc tgcatgaact ccagcggcgg gcagccagcg gatagcgagt caaactggcg
     6361 aattagtgca tcaagctggc tggcgatgag cggttcgcta atcggcaggt aaaactccat
     6421 ctccacctgt ttatcgcggt cggaaagctg actcaggcta acacccgttt cattgagagg
     6481 tgcctggagg acagccgtga tccactcggt caacaccggt tcccactgcg attcaaagcc
     6541 gccgagttcc agtttttcct gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc
     6601 ttcaaacaaa ctgtgcaaga atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt
     6661 taacgtcggt tcgtcaacga cgctgaccac gcctgcggca tcaacatcca gccgtggcat
     6721 cagatcctga gcgataccgt gaccacgctg ctgcaaaccg gagtagctgg tgacacgcca
     6781 gttatcgccg ggcaatcgtt gtagcgtcct cgcattcagt tctgcagtta tagcatcatt
     6841 aacctgccag ggctggttat caccagtttg tgccgtttgc caggcaatat catcatcgca
     6901 taacgcttca atacaggtgc gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa
     6961 acgcccgagc gcactttggt ggacgtcggt gtcacctttt ttatcgccac gacggcgcac
     7021 cagcggtgca acgccgagac tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagcaa
     7081 acgcagatct tccgccagac gttcggcctc cgcgagatcg acgctttctg gcgcagcatt
     7141 aagatccaga acagcctcaa acgagtggcg atcgtgatag aacgcctgat cctggacgcg
     7201 gaaattggta ataaatggca accagaccag cggatattcc agcccttttg atttatggat
     7261 cgtgacaatc tgcaccagat gtttatcact ttcgaggcgc atttgttggc tggaggcatt
     7321 actgtctggc tcgaggatat gttgcgataa ccagcgtacc agcgcatgtt cactttccag
     7381 ctgcgttccg gcttcttgta gcaattcgct gatatgcaag atatcggtaa gacgccgctc
     7441 accgcctgct gttgccagca agttttctgc aatgttacgc gccgacatca gcgcccgcag
     7501 catcggcata acgccacgtt tgcgccagat ttgccgataa ccatcgaact cttcgacgac
     7561 cgcatcccac gcatgttcgt cattattcag cgtttcaata tccagcgcat tcagccccat
     7621 cattgacgtt gccagcgcac tgcgcagggt gttctcacgt tcgggagtca tcaccgcctg
     7681 caacagccaa agcatctcct gcgcttccag agtttcaaaa acactgtcgc ggttcgaaag
     7741 gtaaacggaa gggatttcca gcagcattaa ggcatcgcgc acctgggcgg cctcctggcg
     7801 gctgcgcacc agcacactga tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat
     7861 cagcaacgct tcgccccgct gtccggcttg tagccagtcg cggatttgcg cagcacatac
     7921 ctgcgccatg gtactttgat agtcgccaac gccgcagctt tcgccttcca tcagccacat
     7981 tttcatcgca ggctgcgttt cacctttaaa cacaaaacgt aacgcctgat ttttgccggc
     8041 agatttcaca ggaataaacg gtatttcacg aaacatgaac gcgtcatcag tctggctgaa
     8101 aagtttgtta acgctgttca ccattcccgg tgcggaacgc cagttggtat ctaaggtgta
     8161 gtgagcatga acttcgctac gcgccttcat ataagtgaag atatccgcac cccggaatgc
     8221 ataaatagcc tgtttcgggt cgccaatcag caacaatgcg gtttccggct gatggtgcca
     8281 gatacggcga aaaattcgat actgctgggg gtcggtatcc tgaaattcat cgatcattgc
     8341 caccgggaat cgcgtacgga tcgccgctgc caatacctcg ccgctttcac tacgcagcgc
     8401 ggaatcgagc cgacttaaca tgtcatcaaa acccaattcg ccacggcggc gtttttcacg
     8461 cgctactgtt tcgcggatct cagccaatgc gcgggtgatc accagatcgc ggatcgacaa
     8521 tggttctgca agcagttgat cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc
     8581 ggctttcgtg cgatcttcta agaaacgctg agagaatttt tccagcgact ccggcaactg
     8641 atagctgttg gtctcttctt ctgcccaggc gctgatcttg tcgatccatt tagcctgatt
     8701 gctacggtta aacttgcgtc gatcaatacc agaagattcg atcagcgcat ccagttcacc
     8761 cactgcgtcg cgccactgct gttttacggc atcaatacgc gccacaattt gcgcgtggcg
     8821 ggaagcaagc gtttcatcat cgggcggcgg tgctttgata accggtgctt cgccttgcag
     8881 ataacgatta atatcgcgca gcaacgcctg cggccctttc caggtttcaa agaccacctg
     8941 ggcaatttca cgcggcagcg ggtagcagtg gcgacgccag aaatcggcgc aggcctggta
     9001 gcgtagcaga gactcatctt caatcagctg ctgctcaaac agcatgccgg attcaaaggc
     9061 attcaggttg agcatgcgct ggcaaaaacc gtgaatagta aagactgccg cttcatccat
     9121 ctgccgttcg gctaacaaca accactgcgc ggcttgcgct ttatcgtcga tctcctccag
     9181 caggagtttg tacagtggat tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg
     9241 gatattgcta cggatacgac cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac
     9301 cagcagttct tcaacggtca gcgggcgggg aaaggcggca gaaccgccta gtccaagtaa
     9361 caggcgcaaa tagagcgccg caatcgtaaa ggttttgcct gtgccggcag aggcttcaat
     9421 caggcgctcg ccctgtaagg gcaagcgcaa aggatctagt gtctcggcga catcactcat
     9481 tcttttcact catcaggggc attgtttgct gcaacgcgct gacgttctcc cacactttcc
     9541 agccttcagg atgcacatat tcggctttcc cgttctggct gccggaaatc tgcgacagaa
     9601 tagccatgcc ttgcggctcg accaccgcct gatggaagaa atcagcaagt ttttgcggcg
     9661 tcagcagttt tatctgggcc acgattttat cacgcgaatc gaagcgcata ttgccgcgat
     9721 cgaaatcttt acttaacttc gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct
     9781 gggttattac cgcctgctgg atttgcgcaa actcctctgg cttcatcgcc cgcaattttg
     9841 cctctgcggt tgggaaaaac gccttgtaac gctcccacaa gaatgaaggc tgtttatcat
     9901 tgctttgcaa aaggaagccc atgccccact gacgccccac gctcattgga aacgcaaaca
     9961 cggcatagcc caactgttct tcggtacgca actgattgta gaaccacggc tgtacgatct
    10021 gccccaacag agagctatag gctgagctgg tgtattcatc gtagccagtc ggtacaaata
    10081 ccgctgccag tgcggagtcg gtgctgttac cggctttttc aaagatgaca gattgttttt
    10141 tatcgaccac gacatctttg ttacggcacc actccgaacc atcagcgccc aactgttttt
    10201 gcacatcgcg tgccagcgtt gttgcctggg cttcggtcat gttgccaata accataaact
    10261 ctggtcgagc ccctgatttt aaggcgtcgc gataggccag cacctctttc aaagtgattg
    10321 aaggcaaaat tttgcgccgt tcatctcgcg agaagtacgg cacttgcgag agcatctgcg
    10381 cgggcataat cgcctgctcg aacgctttgc ccttttctgc ggaatccatc atctggttat
    10441 accaggactt cgcctgctca agctgatctt ccgtagcggt atagctaaag tacccctcga
    10501 gcaatgcctg gaacagctgc ggcagacgct gggtgtaacc attagcatta accataaggc
    10561 cgttgttagc gttggtggaa aaactaatgc caccaaccga cgcctggttg cttaactgat
    10621 caagcgctag cccggcaaga taatcattga gcgcaaacat cacctgattg cgggcgctgt
    10681 ccatggcttt cggattacgc aaaatcaggc tgacatcagc tttgggttcg ctggaaaaat
    10741 aacggcttgg cgcatagacc acacgcagat tcgactcatc aacaataagc tctggatggt
    10801 catatttctt ctctgactta atcagcgaga aatcgtcagg aatatagggg ttaagctctg
    10861 gcaaagagag cgcaatgtcg gcggcttttt tctgccagtc agcgaaagtt tgtgcgctaa
    10921 ttttatcgac ctgatacggc gcatcgacaa agtaagccgt tttgttgtgc ggctctttcg
    10981 ggctgatata ccagatacgc gcattctgcg gcgtcatcat cgccaggcgt tcttttactg
    11041 ctttagcgtc gtaccgatcg gcaatattga ctgcatccag cgtatgctca acaggaacgc
    11101 gaatcatggt atctgccagc cattcgacgt aatccatatc acgggtgatt gacggataac
    11161 gaaagtcgat atccagcaca ttcgccagtt catcgaagta ttgtttatca atcccttttt
    11221 cgcgtaacag attgaggtag ctaaaaatgg ccgccacaac ctgatcgcga ttcgccagac
    11281 ctttatcggt taaagacgca gagatcgcta atacgccgct gttgccgttg acgataggat
    11341 cggaattggc gctaatgccc tcaactaatc cctgcttttg cagccagtca gaaagtgtac
    11401 ctgggctgcg attaccgatc agataggtaa tcaattcatc cgttttacta cggaacttcg
    11461 ctgaattgtt atcgatgcga aactcaacgc gcagaacttt acgcggcagc gccgggacgt
    11521 aatgaatgat aatacctttt tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt
    11581 tgctctcttt gttcggcacg cgaccaaagg tgtccgccgc catttttgcc aactccggca
    11641 gcggtttatt gctgtaaata accgccttca tcagattggc agagtaatat ttctcgtgaa
    11701 aatctttcag cgcctgctgc accggatttc cgggtttgtc gcttaaagtt tcgaggttac
    11761 caccagaaaa ctttgaaccg gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca
    11821 tgcgcatccc gtcacgcgta cgcgccatgg ttaattcggc attcactgca ttacgttcac
    11881 gttcggcgta tttcttgtcg agcaagggtt ctgcaatagc gtccgccagg cggtctaccg
    11941 caccgggcaa ggcgtcgttc tcaacttcca gatagaaagc cgtgcgatac ggtgccgtgc
    12001 tggcattgtg actgccgccg tgcattttga gatattcggc cagactgtca gcctgcgggt
    12061 actttttcga ccccatcaga ctcatatgtt caaggtaatg tgccagcccc tggtacgcct
    12121 cgggatcttc cagcgaccca acgggcacta ccagcgccga gagcgattta accgcctgcg
    12181 gatcagaaac cagcaagacc accataccgt tatccagacg tatagcctga tactggcggt
    12241 tatctttatc acttttacgg atggtttcct gaatcggctg ccatcccgtt tctgcctgac
    12301 ttaagggtgc ccaaagggca acaaacaaca ataatgcttt gaaccaggtg ctgcggggca
    12361 ttcacggacc tcataatcaa ctttattttc tggccagatt caacaggtta acgcaaatca
    12421 tctgccagaa tttaatcttg tgctgaacga gtcagcctat gtctatatat ccatcagtcc
    12481 gtgactggtg cgcatcataa agcaagcaga tatattgcgc aatttttata cagcactcat
    12541 gactgattaa agcgaaacag cggtaacagg aaacgttgcg actgttcaac gatagcctcc
    12601 attgtctctg gtgttaattg ccgccagagc cgttgatacc agatatcatc accttcgcca
    12661 cgcaccatca tgttgccttc gtaagcctga aggaatttcg tacgggcttt ttgtaacgtg
    12721 gaatcgtcat ccagcatggc atcgttttgc gcgtcataac aggtttttag ccacgcgccg
    12781 ccactttcag gtaacaccag caatggcgcg gacattcctt cacgataccc ctcaatcagt
    12841 tgtgagaggt aatgcaaagc ctgttcggct gcaagcggcg gaaaacgcca ctcgccgtct
    12901 ttgcgtagaa aaaggcgact ttcaccatta ccaccgctgg cacagtagac aaggtgttcc
    12961 agccaaagtt gcattccctg cgccacactt aataaagagg gacgccagcg caacaggcca
    13021 tccggctgca cctgcggcaa ccagccagtt atctgcacac cgttgcaggc gagatcaatt
    13081 tccatactct gccccggctg gcgacgtgca atgactctgt cggcaagctg ctgcatctcc
    13141 tggcactgtg tttcccagaa aatttcacca aaagcgccat acggtaaatc ccctgccgcc
    13201 cggaagcggc ggaataagcg atcggcatca tcctgctcaa ccagtgcatt caataactgc
    13261 tgattgattt gataacggct aagcccttcc agaataaatg gctcggtgtc ggggatttcg
    13321 ctgtcttcag tacggaagtt cacctgcaaa cgcatccgga agaacgcccg taccggatgt
    13381 gcccagaatc gttgtagcgt ttccagcggc acggtttccg gtaaggtaaa cggcagcggc
    13441 tgaacaaatt cagaatgtgc tttaccagcc tggctggccg caggtagcca ttcacgagca
    13501 tagctttgtc gttcgcctgg ctggtagttt tgcggatcaa acggcatccg ggtatggagg
    13561 caagtaagat gcgcttttac ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc
    13621 ggtagataat ggctttgccc gatgtagtcg atcagttcct gcaccagtac cgacgggaaa
    13681 cgttcactgt tatcctgaat agaacgcccg atatagctga tatagagttt ttgctgcgcg
    13741 gaaattaacg cttccaggaa cagatagcgg tcgtcatcgc gacggctacg gtcgccacgc
    13801 ttcggtttct ggctcatcag gtcaaagccc aatggcgcaa gctggcgtgg ataaacgccg
    13861 tcgttcattc ccagcaggca aaccactttg aatgggatgg aacgcattgg catcagagta
    13921 caaatgttaa ccggtccggc gagaaaacgc tggctgatac gttcctgatc caggcgctgt
    13981 gccagttcat cacgcaatag tgacagcggc accgcgtcgc catactgcgc acctaaacct
    14041 tcggcgataa tcgcctgcca ttgttgttcg atcagcgtca tagccgcttc ggtttccgca
    14101 tccggcagga agaaggcgtt gagcatatcg cgacaaaccg gcaaccactc ttccagcgga
    14161 cgctcctgcg ccagcccgcg acgccagatg tttagctgca ttagcagtga agccagatgc
    14221 cccaccagtt ctgcaattaa gccgctcgat tcatcataag gtagaaccga ttgccactcg
    14281 ccctgcgcgc tctccatcgc gtagcccagc aacatacgcg tcaggccaaa tcgccaggtg
    14341 tgttgtccgg tggcggggag ttccagctcg cgaacgttgt cgtcatctat cccccaacga
    14401 atgccggatt cgttgaccca ctggcgtaaa taacgcagcc cttcttcggt gatgtcaaac
    14461 cgcgccgcca gcaccggcac atccagcagc gccagcacat cctctgacac aaagcgactg
    14521 tcaggcagtg ataacaggct gataaacgct tccaataccg gatgtgactg ccgcgcccga
    14581 cggtcggaaa tggcgtaagg caggtaacga tccgcaggtg cgctaccaaa cacagcctga
    14641 ataaacggac tgtagctgtc gatgtcggcc accatcacga tgatgtcgcg cggagtcagt
    14701 gtcgggtctt cctccagcat cgccagcagg cgatcgtgta aaacttcaac ttcacgctgc
    14761 gggctatggc aaacgtgaaa ggtgatactg ctatccagtg gatcaagcgg gcgtttgtta
    14821 tcgctacggg aaaactcttc gatgtttaca ccagcaacgg cgcggttttc cagttccaga
    14881 atgtcagact gaatgttatg cagcaggtta tctggcgtaa catcgacaaa agcgtccagc
    14941 tcctggctgc tctccagatc agaaaggaga taaatgtagt cgcgcccaag cttgccccat
    15001 gaggccagca gcgggttgcc gacatcctgt tcaccatcgc tgttaaagag ctgcccggca
    15061 ttttcgctgt cgcgaaatag cggtaattct cgatcttcaa aactgtgtcg gcgctggcga
    15121 gtcagtagtt tcgccagata agcaggatct ttaatgtcgc cccagtaata acggcagggg
    15181 ttggtaaaca ggagatggat ttcaatatgt ttacccagcg cctggagtgc ctggagataa
    15241 acaggcggta acgcggaaat accgcatata aagacgcgcg aaggtaaccc cggcgggcag
    15301 gtcgtcgcgg actccagcgt ttcgataaag cgctgataga gattggcgcg gtgccagcgc
    15361 ggttgcccga gttcatgggt atattccacc aacgccttcc acaacggcgc ttgccaggcc
    15421 tgtgcttctc ccagcccttc aaccaaatgt cctatttccc actgtgccag ccagtccgga
    15481 cgatagacca gatactggtc aaacaggtcc gccgcttttg aagaaagctg gaacagtttt
    15541 cgcttgtcgc tatcgtcagt cagataatgc cgcaacaggg taaagtcttc gcgctccaac
    15601 agttgcggca gcagagtcat cagtttccag ctcatgctct gtttgttaaa ggcgctctct
    15661 ttggggattt ccggtaacac ccggacgaac atatcccaga taaagctcgc tggcagcgga
    15721 aaatcaatgt ttgccgcaat accaaacttt tgcgacaggg tcatttgcag ccactgcgcc
    15781 ataccggtac tttgcaccag aatcatttct ggttcgaaag gatcgtccag ccgttcgcgt
    15841 tcgacgataa actccatcaa cgcttccagc acgtccagac gattggaatg gtagaccctt
    15901 aacatagcgg ctcctgacta ctgacgattc gggcaatgca ggcgggtcat ctcgccttct
    15961 ctgcccccgg gtgaaactag cgtaacgctg atgctgacac atcccgcctg cgatgtctgc
    16021 attcggttga cctgccagtt agcaggtggc gaaatcgcgc gcagttgcgt ttgctgccag
    16081 ccatgccgcc agagctgttg gtactggttt ctgctggcaa gactgttcat taatgttcgc
    16141 tggaaacccg ataacgcagt gacaatcatc accatcaaca ccatcgccaa cattacctcc
    16201 ggcagactaa agccttgttg attcctcagg gaagctgaca taacgccctc tctttcagcg
    16261 gacaaaaatc gctccagccg cgtggcgaaa aaacaatgtt tccatcgatg acttcgcctg
    16321 ttcgccacaa cgaaacgcct tcataaccag caatcaataa ggcttcatta tctgccagta
    16381 aacgcagaca aacccgggca ccggtggcag catacagtaa gcactgaact gcgggcagcg
    16441 tctgccagga gtgcattttt ccccactcca gcgccgactg aacgatggcc tggcggcgca
    16501 atgactgact ttccatgctc acgcgagaag caaaactgcg atcctgctga ctcattcctt
    16561 gcaatagcaa gctacccaaa atcagcagca tcaggaccag agccagtgac gaaacaccct
    16621 tttcgcggtt cacaggttgg atcctgtcac gctatagctg gcatccacca cggtttgcgg
    16681 atcagccttg ctggcagcgt gcatattaac cgtcaacacc ggcgagaagc cgctgacatc
    16741 ctgacgtacg acctgaaaag tgtcgataat gatggcatcc ggattagtga ctttatccca
    16801 gcccttacct tcacaggatg tcgcaccgcg tagcgtttcc agcacatgct ccttcagacg
    16861 aaatccaatc tggtcggact cttttaccgg ttcgcgatcc cagataccat tactgttcgc
    16921 atcccactgc acaatgacac agtcaccctg tccgacaatt tccagccctt cgcccgtaca
    16981 gctgccatgg caataacccg ccctctggag atgcttcgcg acggtaaata cccgcagcca
    17041 gatttcatct tccagcgcca gcttacgggt gttcgttaaa ctttcacgct gtaacgcagg
    17101 cagaaagcgt gcagccccca gcaacaatac gctactgatc gccatagcaa tcaacacttc
    17161 cagcagagaa aaaccttgct cttttacagg catccttctg tttctccttg ctgacaaagc
    17221 cggagtcttc cccacggcga aaccactagc caccactcgc ccgttgagtt tttgaagcga
    17281 atatgcccgg cccatgcggt attgcgcagg ccaaagaaag caagcgaagg tgtcaggtcg
    17341 ctcatttcga cttcgggcca gcgcggcaca aagaccaatg gtgaactgcc atgacaggta
    17401 ttcgccccag caacggaact cacaaggcac cataacgtcc cctccctgat aagactgata
    17461 ctgtggtcgc ggttatgcca gttggcatct tcacgtaaat agagcaaata gtcccgcgcc
    17521 tggctggcgg tttgccaaag ccgttgcgac tgctgccagt attgccagcc ataaagccca
    17581 cttgcgctta gcatgaccag aatcagcatc gcgaccagcg tttcaatcag cgtataacca
    17641 cgttgtgttt tcatgccggc agtatggagc gaggagaaaa aaagacgagg gccagtttct
    17701 atttcttcgg cacatcttcc ggactattta cgccgttgca ggacgttgca aaatttcggg
    17761 aaggtgtctc gaagaattta acggagggca aaaaaaaccg acgcacactg gcgtcggctc
    17821 tggcaggatg tttcgtaatt agatagccac cggcgcttta atgcccggat gtggatcgta
    17881 tccttcaatc tcaaagtctt cgaaacggta gtcgaagatg gattcgggtt tacgtttgat
    17941 aatcaacttc ggcagcggac gcggttcgcg gcttaattgc agatgagttt gatccatatg
    18001 gttgctgtag agatgcgtgt cgccaccggt ccagacaaaa tcacccactt ccagatcgca
    18061 ctgctgcgcc atcatatgca ccagtaacgc gtagctggca atgttgaacg gcaggccgag
    18121 gaagacgtca caggagcgct gataaagctg gcaagagagt ttgccgtctg ccacatagaa
    18181 ctggaagaat gcatggcacg gtgccagcgc cattttatcc agttcgccta cgttccacgc
    18241 tgaaacaata atgcggcggg aatccgggtc gtttttcagc tggttcagta cagtagtgat
    18301 ctggtcaata tgacgaccat ccggcgttgg ccaggcgcgc cactgtttac catacactgg
    18361 cccgaggtcg ccgttttcat cggcccattc gtcccagatg gtgacattgt tttcgtgtag
    18421 ataagcaatg ttagtgtcgc cctgcaggaa ccacagcagt tcgtggatga tggaacgcag
    18481 gtggcaacgt ttagttgtca ccagcgggaa tccatcctgc aggttaaaac gcatctgatg
    18541 accaaaaatg gaaagcgttc cggttccggt acggtcgttt ttctgtgtgc cttcgtcgag
    18601 cactttttgc atcagttcta aatactgttt catggttcct caggaaacgt gttgctgtgg
    18661 gctgcgacga tatgcccaga ccatcatgat cacacccgcg acaatcatcg ggatggaaag
    18721 aatttgcccc atgctgatgt actgcaccca ggcaccagta aactgcgcgt cgggctggcg
    18781 gaaaaactca acaatgatgc gaaacgcgcc gtaaccaatc aggaacaagc ctgagacagc
    18841 tcccattggg cgcggtttac gaatatacag gttgaggata ataaacagca ccacaccttc
    18901 cagcagcagc tcgtaaagct gtgatgggtg gcgcggcagc acaccgtaag tgtcgaaaat
    18961 ggattgccac tgcggattgg tttgcagcag caaaatatct tctgtacggg agccagggaa
    19021 cagcatggca aacgggaagt tcgggtcaac gcggccccac aattcaccgt taataaagtt
    19081 gcccaggcgc ccggcaccaa gaccaaacgg aatgagtggg gcaataaaat cagagacctg
    19141 gaagaaggaa cgtttagtac ggcgggcgaa gataatcatc accacgataa cgccaatcag
    19201 gcctccgtgg aaggacatgc cgccgtccca tacacggaaa agatacagcg gatcggccat
    19261 aaactgcggg aaattgtaga acagaacata accaatacgt ccaccgagga agacgccgag
    19321 gaagcccgca tagagtaagt tttcaacttc atttttggtc cagccactgc ccggacgatt
    19381 cgcccgtcgt gttgccagcc acattgcaaa aatgaaaccc accagataca tcaggccgta
    19441 ccagtgaagc gccacgggtc ctattgagaa aatgaccgga tcaaactccg gaaaatgcag
    19501 atagctactg gtcatctgtc accacaagtt cttgttattt cgctgaaaga gaacagcgat
    19561 tgaaatgcac gccgcaggtt tcaggcgctc caaaggtgcg aataatagca caaggggacc
    19621 tggctggttg ccggataccg ttaaaaaata tgtatatgat ccgcgctata accccccgcg
    19681 aatcaacccg cccatgccgc gacgctccat aaaggctgca acctgatggc gaacttcggt
    19741 cgccagttgc gcttccagac tacgccgcgc aagattttct gcttcagcaa aatcaatgcg
    19801 ccgcagcagg tattttacgc gcgctacaga acgtccgttc atagacagat ggcgataccc
    19861 aagcccaatg aggattgcca cgcacatggg atcgcccgcc atttcaccgc acaaacggag
    19921 atcgattcca tgtatttccg cttcccgggc gatcatcgcc agagctcgta acattgcagg
    19981 atgaagactg tcataaatgt tcgccacccg ggtattgttg cgatcaacgg ccaggatgta
    20041 ttgagtcaga tcgttggtgc caacagagat gaaatcgacc cgctttgcca gatgcggcag
    20101 cataaatacc attgacggca cttccagcat gatgccgata cgtggtttgg gaatttcgta
    20161 accgatcatc tcctcgactt cacgtccggc acgttcaatc aggcggcgtg cttcgtcaac
    20221 ttcatcgagg cttgtgacca tcggcaacag aatattcagg ttgcccgtag cggcattagc
    20281 acgcagcatc gcccgcacct ggatcaagaa gatctccggc tgatcgagcg taatgcgaat
    20341 cccacgccaa cccaggcatg gattctcttc gctaatcggc atgtaaggca gctgcttatc
    20401 tgctccgaca tccagcgtac gcaaggtgac gggtttatca ttaaacattt gcagcatccc
    20461 ctgatactgc gccacctgtt cttcttccga cggaaaacca ctttgcagca tgaatgggat
    20521 ttcagtgcga taaagtccta tgccatcaat acggctgccc agtttttctt catgttccgg
    20581 gcttaaaccg gcattgagca tgactttaat gcgctcgccg ctttttaact gggcgggtaa
    20641 attgacgtca tcttccgcca gacggctaag ctcgatctct tcactaatta gccgctgata
    20701 ttcttgcagc agtaccggtt ccggatcgac cagcaattca ccgcgataac catcgacgat
    20761 cagcgtccga cgatgcagta ccgaaggctg aatatccgcg cccatcacgg taggtatccc
    20821 cagcgcacgt accatgatcg cagcatggga gttggcagcg ccatcgcgca cgacaacacc
    20881 gactaagcga tcctggggca gctcagcaag cgttgtcgct gacagttcat ctgccaccag
    20941 aatgaaacgt tccggccagg cgttcggccc ttgattagcg tcatcaagat gaaacagcaa
    21001 tcgctgaccc agcgcacgta aatcgccagc ccgctctttg agatagttat cgcttagcgc
    21061 ggcaaactgt tcggcaaatt tttcaatgac cgtttttacc gcccactctg ccaccgagcc
    21121 tttatcaacc tcggcaaaca attcgcgacg cagccgggta tccgaaagca ggtgtgagta
    21181 aagatcgaaa atagccgccg tttctttttg tgcaccggca gcaaagcgtt tgctatagcg
    21241 gcgaaactcg ttagccgcct cttccaacgc cccggtcagt cgttcgcgtt ccagagccgg
    21301 atccagcgtt gatgcctgat acacctgttc cattaaaggt aacgtggcat cctgccagcc
    21361 ttcggcaatc gccacaccag gtgctgccgg taatgcacgg attcgcgtct ggcgatattg
    21421 cccaaacaag gcagtcaact gcgactgaga aagaatagct gccatctggg tggcaagcgt
    21481 caccaggaag gattcttcac tttcgtcata ctggcgcaac tctcgttgct gtaccaccag
    21541 tacaccaagc aactggcgac gttgaataat tggtacgcct aaaaacgcgc ggaaacgttc
    21601 ttcttttacg gaggggatgt atttgaagct ggggtgcttt tgcgcatctg caaggtttat
    21661 cggttccgcc agcctgccaa ccaggccgac gatcccttca tcaaacgcga gcgttacagt
    21721 gcgaccgcgt ggttttttca gtccccgggt cgccatcagg tagtaacaac gtcgatcatg
    21781 atcggccagg tagaccgaac agacctcggt atccatcgca agacagatgt cggtaaccag
    21841 aatatttaac gcctcattca ggcgtggtgc gctggctacc ttttcgacta tttcgcgcag
    21901 gcgagtgagc ataattggcg tgacttaacc tcttttacgt cgataagcag atgcgttttg
    21961 tggttttggc gtattttcct gcagtgacat caccacactc gcgaactctt tcattaccct
    22021 acggtagaca tcacgtttaa atgacaccac ctgtctgact ggataccagt aacttaccca
    22081 tcgccagccg tcaaactctg gtgtactgct ggtttgcata ttgatttctg catcgccgct
    22141 caccagctgc aagagaaacc atttttgttt ttggccgata caaaccggct tcgtgtccca
    22201 acgcaccaaa cgtttcggta atttgtagcg caaccagtta cgcgttgaag caaggattcg
    22261 aacgtctttg cggcttaatc ctacttcttc aaacaattca cggtacatcg cctgctctgc
    22321 ggattctccg gggttgattc cgccttgcgg aaattgccag gagtgctgac caaatcgccg
    22381 ggcccacatt acctgcccct ggcgattaca aatcacgata cctacgtttg ggcggtagcc
    22441 atcgtcatca atcaccggac tacctcaaaa taaagcttta tatacgaatg attgtttcat
    22501 actccaggaa gacggtaaac cactctctgc agggcattac acactaataa caattgaata
    22561 actcacagtt atgtgcagag ttataaacag aggaaggggt ggatagccgt ttttattcac
    22621 cttttctgtg gatagagttg tgaagaacta cggaattact acgggaaaac ccggagcatt
    22681 ctgaataaga cagatacaga gattttaaaa tattcattat cagtcaatgc attacatgtg
    22741 atttgagtaa taaaaaagag taattcgtga cccaggtcac acctctcatt tacgggttgg
    22801 cgaaagatca accaatgccg tatttatcca cagaatgtgc cactaagtta agcactgaac
    22861 cactaaaaac tggagtttcg tcgcacgtca aggctgtaaa tggaaacagt agtgaaggtt
    22921 tttcacagtt atcccagctt tctgtggata acatggtgta agatcctgtt tattttcagt
    22981 gaccagattt ggaaaacccg ttgcagtgtt gcgcaactcg attaccggca acctaaaaag
    23041 caatataaat cagagaattg aacaacgcat gtggaaaaag ttacactgcg aatattcggc
    23101 acttaattgc tgtttgtttt ttaaccaagg tatcatgaca tgtcccaacc tcgcccactg
    23161 ctctctcctc ccgaaactga agaacaattg ttagcgcaag cacaacaact ttctggttat
    23221 acattgggag aactggcggc acttgccggg ctggttacgc cggagaattt aaaacgcgat
    23281 aagggctgga ttggcgtgtt actggagatc tggctaggtg ccagcgcagg gagtaaacct
    23341 gagcaagatt ttgctgctct gggcgtggaa cttaaaacta tccctgtgga tagtcttggt
    23401 cgtccgctgg aaacaacatt cgtttgtgtt gccccgttaa cgggcaatag cggggtgacc
    23461 tgggaaacca gccacgtgcg ccacaagctc aaacgcgtgc tgtggatacc ggttgaaggc
    23521 gagcgcagca tcccgctggc aaagcgtcgc gtaggatcac cgttgctgtg gagcccgaat
    23581 gaagaggaag accgacagtt acgcgaagac tgggaagaat taatggatat gattgttctc
    23641 ggtcagattg agcggatcac cgctcgtcac ggggaatatt tacagatacg accgaaagca
    23701 gcgaatgcga aagcgctgac cgaagctatt ggtgcccggg gcgaacggat tctgacgctg
    23761 ccgcgcggat tttatttgaa gaagaatttc accagtgcgc tactggcccg tcattttctg
    23821 atccagtagc catcgctttg acctgccgct ttccgggcat ataattaccg cttcattttt
    23881 ttggcagggc tttttagatg ttatttgcat ggataaccga tcctaacgcc tggcttgcgc
    23941 tcggtacact gacgctgctg gagatcgttc ttgggatcga caatattatt ttcctttctc
    24001 tggtggtggc aaagcttccc acagcacaac gtgctcatgc gcgccgtctg gggttggcgg
    24061 gagccatggt catgcgtctg gcgctgctgg catccatcgc ctgggttacg cgcctgacga
    24121 atccgctttt tacaatattc agtcaggaaa tttccgcccg tgatttgatc ctgcttctgg
    24181 gtggcttgtt ccttatctgg aaagccagca aggaaatcca cgaatccatc gaaggtgaag
    24241 aagaagggct gaaaacacgc gtttcatcat tcctcggcgc tatcgtgcag attatgctgc
    24301 tggatattat cttcagcctc gactcggtga ttaccgctgt gggtctgtca gatcacctgt
    24361 ttattatgat ggctgccgtg gtgattgccg taggcgtgat gatgttcgcc gcgcgctcga
    24421 ttggtgattt tgtcgagcgc catccttcgg taaaaatgct ggcgctctct ttcctgattc
    24481 tggtgggctt taccctgatt ctggaaagtt tcgacatcca cgtaccgaaa ggttacatct
    24541 acttcgcgat gttcttctct attgcggttg aaagcctcaa cctgattcgc aacaaaaaga
    24601 atccgctctg atactccgta cgctctcctg cgggagggcg tactcactcc tgttaccctc
    24661 acagtttaag attttcctgc tttcaagata tataacgtcg gtttataaac agactattat
    24721 cataggtggg ccaaatgagg ataaccggat gaaaaaatgg gcagtaataa tttctgcagt
    24781 cggactggcg tttgctgttt ccgggtgttc cagtgattac gtcatggcga ccaaagatgg
    24841 ccgtatgatt ttgaccgatg gaaaacctga aattgatgat gataccgggc tggtgagtta
    24901 tcacgatcag caaggtaacg cgatgcaaat taaccgtgat gatgtttcgc aaattattga
    24961 acgttaacaa ataaggtcag catccggctg gccttaagat ttttctcttt cccttttccc
    25021 ttccctctgc catttttata ttccttatgt cgtgattata aaaaggaaac ggctatgcaa
    25081 tatcaccgta taccccacag ttcgctggaa gtcagcacgc tggggcttgg cacgatgacg
    25141 tttggtgaac agaacagcga agccgacgcc cacgcacaac tcgactatgc cgtcgctcag
    25201 ggcattaacc ttatcgacgt tgccgaaatg tacccagtac ctccgcgccc cgaaactcaa
    25261 gggctaaccg aaacctacgt cggcaactgg ctggcgaaac atggcagccg cgaaaagtta
    25321 attatcgcct ccaaagtgag cggaccgtcg cgcaataatg acaaaggcat ccgcccggat
    25381 caggcgctgg atcggaagaa tatccgcgaa gcgctgcatg acagcctcaa gcgtctgcag
    25441 actgattacc tcgatcttta tcaggtgcac tggccgcagc gcccaaccaa ctgcttcggc
    25501 aaactcggtt atagctggac agattctgcg cctgcggttt cgctgctgga tacactggac
    25561 gcactggcag agtaccaacg cgcgggaaaa attcgttata tcggcgtgtc gaacgaaact
    25621 gcatttggcg taatgcgcta cctgcatctg gcggacaaac acgatctgcc gcgtattgtc
    25681 accattcaga acccctacag tctgttaaac cgcagttttg aagtaggtct ggcagaagtc
    25741 agccagtatg aaggggtcga actgctggcc tattcgtgcc tgggtttcgg cacgctgacc
    25801 gggaaatatc tcaacggtgc aaaacccgct ggcgcacgta atacgctctt tagtcggttc
    25861 acccgctata gcggtgagca aacgcaaaaa gccgtcgcgg cgtatgttga tatagccaga
    25921 cgtcatggcc tggatcctgc acagatggcg ctcgcttttg tacgccgtca accgtttgtt
    25981 gccagcactc tgctgggcgc aaccacgatg gagcagctga aaactaacgt cgaaagtttg
    26041 catctggagt taagcgaaga cgtattagca gaaattgaag cggtgcatca ggtttacact
    26101 tatccggcac cataagatta acgccggtta aaaccggcgt taaatattaa taacggcgct
    26161 gccagatcca cagcgccgtt attgccagcg caaacagcgc accgaagcca atgccaatgg
    26221 gcacgaccgg gatgcctacc attaccgcca gcgagtaaat gcccagcatc aacaacatgg
    26281 cgctgttttc gccaaggttt tgtactgcaa tcgcattccc cgccccgacg ctttttttac
    26341 cccgttcctg tagcaacgca ttgagcggaa cgacaaaaaa gccccccagc acgccaatca
    26401 gcatcaacaa ggcataggct gacagcagct cgtgttgcag ggagaaaatc agtaccacca
    26461 cgccaatcaa aatcccggct ggcatacagc gtgacacggt ttccagcgta actaacttcg
    26521 ctgccgcacc agcgccaacc acgataccaa tcgctaccat cgcgttgaga taggtcggcg
    26581 tagcgttatc ggtaatgccc agcgccaccg gtacccacag caccaacagg aaacgcagcg
    26641 tgacacccgc tccccagaat aaactggtgc ccaccagcga aaaacgcgtt tcaccattgc
    26701 gccatagcga ggtgcaggca ttcaggaaac tgcgggtcat gctgatgaga ttccaggact
    26761 gccccggacg cgccgccgcc agtttaggaa tgtagatatt ggcaacgacc gcaccaccgt
    26821 aggccagtgc gcatgcggcc agggcgacca ggacatgcca gtcagccagt acaccaccgg
    26881 ctacggaacc gagcaaaatc gccgctatgg tagaagcttc cattaaacca ttagctttca
    26941 ctaacttact acccgtggtt aattcgccga gaataccgta tttcgccggt gaataggctg
    27001 cagcaccaac acccaccagc gtatagccga gaaacggatt gataccaaag cagatactgg
    27061 ctgcgcccag cagcttcagg ccgttggcaa acatcatcac ccggcctttg gcgaagctat
    27121 ccgccacctg tccgacaaac ggcgcaaaaa gaatgtaagc acctacaaac accatttgca
    27181 ggatgggctg gctccactcc ggataaaact gcgctttcag taacgccaga gtggcaaaca
    27241 gcagcgcatt atcgccaaac gcagagagaa actgcgccac gataaccgct ttcatcccct
    27301 tcgaccacaa cgaagtgtta gtgtgcactg actcactcat cgtgttgttc cgcttcgtct
    27361 acccagcttt tcaacgtgac aaagtcaggt ttgccgctgc caagtaatgg catctgtttc
    27421 agatagcgaa tatcgcgcgg tacagcaagc tccggcacgc cgtgctcgcg ggcatactgt
    27481 tgcaacttat cgcgcgtcag ttcgttatct gtggtgaaaa gcaccagtgc ctcgcctttg
    27541 ctggcatcgc tcttaatcgc agtggcatgg actttatctg gcgaaacacc aagtgccagt
    27601 tgttccacca tttccagcga caccatttcg cctgcaattt tggcaaagcg ttttgcgcgg
    27661 ccctgaatct gcacaaagcc ctgctcgtcg aaacgcacaa tatcgccagt gtcataccag
    27721 ccgcgctcca tttcgccgcg aacattctcg gcggtgggca cttccagtac acctggcttc
    27781 tccacccgca gatagccgtt cattatgttc ggccctttca gttgcaggcg tccgccctct
    27841 tcgataccag gaaccgacaa caggcgcgca tccattcctg gtagaatacg ccctaccgta
    27901 ccgggtttcg ccgccatcgg tacgttgata gaaacgacag gcgcgcactc ggtcacaccg
    27961 tagccttcaa ggatgcgcag gccaaattta tcctgccaaa gctgtttggt actttcttgt
    28021 agtttttctg cgcctgccac cacatagcgt agacggtaga agtcatacgg gttggcgaaa
    28081 cgcgcgtagt gaccgagaaa agtcgaggta ccgaacaaca cggtgcaact gcggtcatac
    28141 accaactccg gcacaatgcg gtaatgcagc gggcttggat aaaggaacac ctctgcacct
    28201 gtgagcagtg gcgtaaacag gcctaccgtc agcccaaagg agtgaaacag cggtaacgcc
    28261 gacataaagc gatcgttggt ggtgaagtcg gcaatcgttt taatctgctc gacattcgcc
    28321 agaatgcttt tatggctatg gacgacgcct ttcggatggc cttcagaacc ggaggtaaaa
    28381 aggatcagcg cctcttcttc cggctgctgt tttacctgtg ccagacgcgg catcagcaaa
    28441 tgagcgaaga tccatacttt gtcggcagtg gtgacatctg cttttaaatc ttccagatag
    28501 acccagcgca cctgagtaag ttgctccggc agatgccaga gtttgccttt atcgagaaac
    28561 tgacgggaag tgaaaatggt tttgatttca gccgccgtaa tagcactggt caaccctttt
    28621 accccggcgg tgtagttcat cattgcggga atgcggcgac gggcgatggc cccaaaaatc
    28681 actgccgcac tgatgcctgc attgggcagc attaagccga tgcgttcgcc ttcaacactg
    28741 tatttttcaa ggatgcgtcc aacaaacagc gttttcgtaa gcaatttgcg ataggagtcc
    28801 ggggtaaagt taacgtcttc gacacatttc ttcccggctc cgaagcggta cattgcgctc
    28861 agtaaagatt cgtacagcgt ttcacgcggg cgcaccgcca tacgcgcttc catcattatt
    28921 tgatgcagca tttcgccagc gattttgcga cggtcacggg cacgcggcgc atccggcatc
    28981 tccacctgcg ttggtggcaa aatatgcaga gtaatttgcg ggaacaagcg acgtttaacc
    29041 agacctttca ggcggctgaa gtgcgtaagt tccgcccctt caatacgcac aggaataacc
    29101 gttgcaccag acttcgccgc gacaaatccc gcgccatcgt agattttcat cagcgagcca
    29161 gtggtggtga tgcgtccttc agggaaaatc accactggtc ggccctgttc caccagacgt
    29221 accagatgtt taatcgccat aggttgcgtc gggtcgagag gaacaaagtc gataaatgat
    29281 ttcagccaac gcatatacca ctgttggctt attgaggtgt aaacggcaaa taccggacgt
    29341 acgggtaaaa acaatgccag cagtatgcca tcaataaaag agacgtgatt aggcgtgatt
    29401 agaacgcgct cgcccttcag tgcctgggtg tcacccgtaa cgcgaacgcg atacaaaaca
    29461 cggcacaaat ttcgaaaaaa gctaaaaagc attccaactc cctttgctct gattcagtaa
    29521 aagcgaatgg agggagatta cacgagataa agaacgcgag cgacagtaaa ttaggtgcga
    29581 aaaaaaacct gcgcatccgc gcaggttggt gcaagagaca gggtacgaag agcgtaccga
    29641 ataatctcac caatcaatac ctctgggatc ttgattgtgg tctgcacgac gactcttcgc
    29701 cagtgagaaa acgcaaagga atgaagggaa atgcaacgag gtgtgtaaat tgtcggttac
    29761 tgttacagat tgatgaccgg caaaaaaaaa cctgcgcatc tgcgcaggct ggtgtaattc
    29821 atgtgctcaa cccgaagttg acttcaccta tcaatacctc tgggatcacc actttagcaa
    29881 cctgaagcca aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag
    29941 gtttgcaatc tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacaatgaaa
    30001 acacgccgcc ccttgaacca acgggcgttt tccgtaacac tgaaagaatg taagcgttta
    30061 cccactaagg tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag
    30121 tcgccaccgt ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggcttg
    30181 ctgtgcatag tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg
    30241 cgcagcagac cactgaaacg gtcggtctgg tcgttggtga tgtttccgat ccgtttttcg
    30301 gtgcaatggt gaaagcggtc gaacaggtgg cttatcacac tggtaatttt ttattgattg
    30361 gcaacggtta ccacaacgaa caaaaagagc gtcaggccat tgagcaactg atccgccatc
    30421 gctgtgctgc gttggtcgtc catgccaaaa tgatcccgga tgccgattta gcctcattaa
    30481 tgaaacaaat gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt
    30541 gtattgctct ggacgatcgt tacggtgcct ggctggctac gcgtcattta attcagcaag
    30601 gtcatacccg cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc
    30661 tgcaagggta ttacgatgcc cttgctgaaa gtggtattcc ggccaatgac cggctggtga
    30721 catttggcga accagacgaa agcggcggcg aacaggcaat gaccgagctt ttgggacgag
    30781 gcagaaattt cactgcggta gcctgttata acgattcaat ggcggcgggc gcgatgggcg
    30841 tgctcaatga taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg
    30901 tgctggtgtc acgctatgtg cgcccgcgtc tgaccaccgt gcgttaccca atcgtgacga
    30961 tggcgaccca ggctgccgaa ctggctttgg cgctggcgga taatcgccct ctcccggaaa
    31021 tcactaatgt ctttagtccg acgctggtac gtcgccattc agtgtcaact ccgtcgctgg
    31081 aggcaagtca tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat
    31141 tcttcaatgg tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct
    31201 ggtaacagcg gacggctgtt gtagttggat gacattgatg cgccatatgc ccctgtatca
    31261 tgcagtacca gataatcacc tgccttcact tccggcaagg cgcgggtttc gacatttccc
    31321 ccttcctgct gggtaaagac atcgcccgat tcacataacg gcccggcgac gacggtttcc
    31381 accgttggtg cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa
    31441 ctaccgtaca ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg
    31501 cggctaccca tttgtttgac gctccgcact tgagtaatta atacgccaga ctgcgctacc
    31561 agaaagcgac ccggttcaat ttccagtttc acagggtggc ccaaatggcg ggcgatttgc
    31621 tgacgcgcgg cattccacag accataataa tgctcggtat caaccgcctc ttcaccctgt
    31681 tgataaggaa cagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg
    31741 aggacctgac gcaccatagc accacatacc tgttccagat gggcataatc gacgccagaa
    31801 ccaatgtgca tgtgaatgcc gaccagctgt agatgatgac gttgtatcac gtccagtgcg
    31861 gcgggcagat cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt
    31921 tggctatgcc cgtgaccaaa ccccggatta acacgcagcc atacccgatg ccctggcgaa
    31981 acctgaccca gttggtcgag catatcaaca gaacccgcat tcaccggaat ttgcaattca
    32041 ctgacgcgtt caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcaggg
    32101 tgcgtttgcg gattgtaacc cgccgccagt gcacgctcta tttcgcctaa cgagacggaa
    32161 tccactttca cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc
    32221 tgtgcgaagc gcaccacatc aaactgtttc agcgctgcaa tctgccgacg aataatttgc
    32281 gcatcgtaga cccacaccgg gcagccaaat tctgcgggca aacgcagcag attttcggcg
    32341 gtgagatcgg tatcagtgct gaacagtgaa tgtggcataa caaactccag ataagtgctt
    32401 ttttatgatt acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgagtctatg
    32461 caaaaatgat atggattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt
    32521 catattgaaa tttttcatgc ggtaatgacc gccggaagcc tgactgaggc ggcacacctg
    32581 ctacacacct cacagccaac tgtcagccgc gaactggcgc gctttgagaa ggtgatcggg
    32641 ctgaaattgt ttgagcgcgt acgtggacga ttgcatccta ccgtgcaagg actgcgtctg
    32701 tttgaagaag tgcaacgatc ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt
    32761 ctgcgcgagt ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct
    32821 tttttaccgc agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc
    32881 gtgccccagg aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga
    32941 ctcacggaga cactccatac gcctgcggga acagaacgta ccgaattact ctctttagat
    33001 gaagtgtgtg tgttacctcc gggccatccg ctggcggtaa aaaaggtatt aacgccggat
    33061 gattttcacg gtgaaaacta catcagcctt tcccgtactg acagctatcg ccagttgctg
    33121 gatcaattgt ttactgagaa tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc
    33181 gcgtcagtct gcgcaatggt acgggcgggg gtaggtgttt cggtggttaa cccgctcacc
    33241 gcactggatt atgcggcaag cggtttagtg gtacggcggt tcagtattgc ggttccgttc
    33301 accgttagcc tgatccgccc cctgcaccgc ccgtcatcag cgctggttca ggcgtttagt
    33361 gagcatttac aagcgggatt accgaaactg gtcacttctc ttgacgctat tttgtcgtca
    33421 gctacgacag cataaaagcg acagcatcct cggcatggat cgccgcagta tcaaacacag
    33481 gcagaacact gcgctcttct ggcaccagta aaccaatttc cgtacagcca aaaatgacac
    33541 cctgtgcgcc ctgttctgca aggcgagcaa tcacttgcgc ataataagtg cgtgacgctt
    33601 cggtaaattg ccccagacac agttcttcaa aaataatctg attaattttc gcccgtttat
    33661 ccgcttcagg aatgagacag ttgatggaaa attgttccgt cagccgcccg cgataaaaat
    33721 cctgttccat ggtgtaacgc gtacccagca gcgccacacg agtcattcca gcccggataa
    33781 ttgcacgtcc agtggcatcc gctatgtgta agaaaggcag agagcaacga gactcaatgg
    33841 catccgccac tttatgcatc gtattggtgc ataacacaat accttctgcg cccgcccgct
    33901 gtaagccaag cgccgcctca gccagaatat ccccggtttt gtcccactca ccgcgacgct
    33961 ggcactcttc tatttcatga aaatcgacgc tatgtagcag cacttgcgca gagtgaagcc
    34021 caccaagccg ctgtttaatg ccttcattta tcagacgata gtaaggaata gtggattccc
    34081 agctcattcc tcccagcaaa ccaattgttt tcattcctct ctcctgatgt gtgttaccgc
    34141 aacaaggctg aataaggaac gagacaaacg cctcaacggc caggtgccta atctctatta
    34201 acgaaaaaag ggccggatgt acagcacatc cggcccgtga aatcagacgc cgatatttct
    34261 caacttttcg cctgccatca gtttgcgctc gatatgttcc agcgtgacat ttttggtttc
    34321 cggaatgagc cagaaagtga tgcccacaaa cgcaatgttc agcgcagtgt agagccagaa
    34381 cgtaccggca gcgccaatgc tatcaagcag tgtcaggaag gtcgcgccga taatcatatt
    34441 cgacacccag tttgtcgtcg ttgagcaggt gatgccaaaa tcgcggcatt tcagcggctg
    34501 aatttcagag cacaagatcc acaccactgg cgcggcgctc atcgcataac cggcaataca
    34561 catcatcgtc atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa
    34621 ctgcatcagg cagtaaccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt
    34681 cagagccggc ttacgccctg ctttatctac cgtaaagacc gcaatgaagg tagcgaacat
    34741 aaaggtcagc cccacgacca gagtcgcaat catctgttgt tctgtggtcg taaagcccgc
    34801 cattttgaag atacgtggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat
    34861 cgcctgcaac aacataccga gaaacacagc acgacggacg ttacggttga tcttaaacag
    34921 tgcccaaccg ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc
    34981 tttttccgac gtatcgcgca gcatacgcaa cacttcttcc gcctcaatat gacgcccttt
    35041 ttccgccagc cagcgcgggc tatttggcag gaagaccacc agaataatca gcaggactgc
    35101 tggtaaagca agaaccccca acattgcgcg ccagttaccg ctataactga acgctgtatc
    35161 ggataaaaac gccagcacga tgccgagtgt gaccatcaac tggtacatac tgatcatctt
    35221 accgcgaacg ttttcacttg ccatttcaga aagatacaga ggagcggtgt aagacgcgat
    35281 cccgacagca atgcccagca ccacacgagc ggcgattaac atctctacgc tggtcgcaaa
    35341 tgcggaccct atcgaaccga gcacaaaaag gatagccccc gccatcaggc tgtatttacg
    35401 ccccaggcgg aacgacagcc acccattaaa cagcgcacca attgctgcgc cgagcatcat
    35461 gctactgacc acccattcct gcaaacggct ggtcagcaca aagtgatcgg taatgaacgg
    35521 caacgctccg gcgattacgc cgatatcaag accaaataac aatcctgcga ccgcagcagc
    35581 taccgaaaca aacatattca tacgccgcgt atcccgcaaa gaacgtggcg ttaaagcaga
    35641 ttccgtattg atagtaacca ttttttcctg ccagcagaga gtaagacata gtgaaaaaat
    35701 acgtgaacaa ttcacacagg tgtcaggtcg gaaatagcat aaatatggat taaattgctg
    35761 cgacatgtcg ttatgtgatg gatattccaa ttttcaaatt aagttgaatt attgagatta
    35821 ttattaacca cctaatttta aagcagataa aattcataaa gttcattaat tgataattga
    35881 tatggattat ttcataacca tgatatggat tatgatgatc tacaggtata aaaaaccctg
    35941 ccatgcggca gggtcataaa agtaagagaa atgaattaac gagccagcca accgccatcc
    36001 acggcaatgg tataaccatt cacataatct gaagcgctgg aggcaaggaa cactaccggc
    36061 cccatcaggt cactcggcag tccccaacgg ccagctggaa tgcggtcgag aatttccgcg
    36121 ctacgttgtt catctgcccg tagttgttga gtattgttgg ttgccatgta acccggagct
    36181 atcgcattaa cattaatgtt gtgtttagcc cattcattcg ccatcaatcg cgtcacgccc
    36241 atcacgccgc tttttgatgc ggtataagaa ggcacacgga tcccgccctg gaaggagagc
    36301 attgacgcga tattgataat cttgccgcca ttgccttgcg cgataaagtg tttcgccgct
    36361 gcctgagaca tgaagaatac gctcttgata ttcaggttca tgacatcgtc ccagtctttt
    36421 tcgctgaact cgagagcgtc ttcgcggcga atcaatccgg cgttattcac caggatatca
    36481 atatgaccaa actcagctac cgcgcgatcc agcagtgctg gaatgccatc aatctttcgc
    36541 agatcggcgg tcaggcttaa aaaacgacgc cccagcgccg tgacctgctt gatggtttca
    36601 gtcggttcaa cgatgttaat gccaacaatg tcacagcctg cttgcgccag ccccaacgcc
    36661 atcccctggc ccagcccagt atcacaacca gtgacgaccg caactttacc ttcgagagaa
    36721 aatgcactta aaatcataac aataccttat tcgtttatgc ccacaactag cgcaaatctt
    36781 taacggccac atggtccata tcatcaaaaa cctggttttc accgaccatg ccccagataa
    36841 aggtataagc tttggttccg acgccggaat ggatcgacca gctcggggag atcaccgcct
    36901 gctcgttatg catcacaatg tgacgcgttt cttgcggttg ccccatcatg tggaaaacgc
    36961 aggcgtcatc atccatattg aaatagaaat aaacttccat ccggcgctcg tgggtgtgac
    37021 acggcatggt gttccacaag ttacctggag ccagctccgt cagccccata ctcaattggc
    37081 aggtttccag cacatccggg acaaaatatt tgttaatcgt gcgacggtta ctggtgaggt
    37141 tatcgcctaa cgtgactgga gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg
    37201 tatgcgcggg tgcgcaattg taataaaact tcgccggagt ggcggtatca atactggcaa
    37261 agacaacttc ttttgcacct ttaccaacat acagggcttc gcggtgaccg atttcatagc
    37321 attgaccatc gacagtaatc gtaccggcac cgccaatatt gataatacct aactcgcgac
    37381 gttcaaggaa atagcttacg ccgagttgtt taccaacttc cccgccaacg gaaaccgttt
    37441 tagttaccgg cataatgccg ccaacaataa tacggtcaat gtggctgtaa accatggtgt
    37501 actcatcggc gacaaatacc ttttcaacca aaaattcatt gcgcagcccc tgggtatcca
    37561 gcgtttttgc gtgcgcactg tggatgctct gtcttacgtc cacatcaacc tccgataatc
    37621 agtgaagcgt gaaaatagaa aacgatagtg aaacgctgtt tcgttttctc tataagggga
    37681 tgatatgccc acgggatagg gttttcaatt aaaaataaaa caatgtttca tttttaaggt
    37741 aaggactgaa aaaagtgcag tgtcgatcac gaatatgtca tgagaatgga aaaatcaggg
    37801 agccctgaaa aggtatgtcg actcgccagc caccattatt caacagatta atgacggctg
    37861 atatttatcg gtaaggagga aatgcagagc tattcgtcac gttcaatggt caatgccacg
    37921 ccctggcccc cgccgataca aagcgtagcc agtcctttgc gggcattacg tttcaccatt
    37981 tcatgaacca gagaaacgag gattcggcaa ccggaagcgc ctattgggtg accgagtgcg
    38041 atcgcgccac cattgacatt gacacgacgc tcatcccatt caagcatctt gccaaccgaa
    38101 agcgcctgtg cagcaaacgc ttcattagcc tcgataagat cgacatcagc caactgccag
    38161 cctacacgct ccaggcaacg gcgggtcgca tacaccggcg caattcccat caatgccgga
    38221 tctacaccaa cgctggcaaa tgcgcggatg cgggccagca cgggtaaatt caacgctcgt
    38281 gctttggctt cgctcatcat cattactgca gctgcgccat cgtttatgga tgatgcatta
    38341 cccgctgtca cagaaccgag actatcaaat gaaggattta aacgggctaa gccttctgca
    38401 ctggcgtcag tgcgtggctg ctcatcggta tcaacaacca acgtctgacc gttactttgg
    38461 gtcattaccg ggacgatctc atctttaaat cgtccagcgt caatcgccgc tcgcgctttt
    38521 tgttgcgagc taagtgcgta agcatcctgc aactgacggc tgatgccata ttcgcgagcc
    38581 agattttcgg cggtgacacc aatatgataa tcattgaagg catcccacaa cccatcgtgc
    38641 acaagactat caaccaactg gctattgcca agctgtgcat cggtgcggct atcagtcaga
    38701 acatgtggtg cgcggctcat gttttcctgg ccaccggcga tgacaatatc agcctcgcca
    38761 cactgtatcg cctgagtagc cagatgcagt gctttaagcc cggaaccgca aacgtcatta
    38821 atagtgattg cagaaacgct attaggaaga ccacctttaa tagccgattg ccttgccgga
    38881 ttctgccctg cccctgcagt caacacctga ccaagaatta cttcatccac cgcatatgca
    38941 ggaacgccgg tacgttctat taacgctttc acgaccaggc tgccaagttc cacggcggaa
    39001 tgacccgcta acgcaccacg gaagcagccg atgggtgtcc gtaacgcccc gacaatcaca
    39061 acgtctttca tcacaacctc gcaccaatta acagtgcaat agt
//
LOCUS       DAZGAP010000017        38423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGAP010000017 DAZGAP010000000
VERSION     DAZGAP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.017"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            212..1867
                     /gene="aslA"
                     /locus_tag="ACHLAM_000778"
     CDS             212..1867
                     /gene="aslA"
                     /locus_tag="ACHLAM_000778"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO7852353.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(2025..3260)
                     /gene="aslB"
                     /locus_tag="ACHLAM_000779"
     CDS             complement(2025..3260)
                     /gene="aslB"
                     /locus_tag="ACHLAM_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7852354.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKVFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3407..3483)
                     /locus_tag="ACHLAM_000780"
     tRNA            complement(3407..3483)
                     /locus_tag="ACHLAM_000780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3447..3449),aa:Pro,seq:tgg)
     gene            complement(3526..3612)
                     /locus_tag="ACHLAM_000781"
     tRNA            complement(3526..3612)
                     /locus_tag="ACHLAM_000781"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3576..3578),aa:Leu,seq:cag)
     gene            complement(3633..3708)
                     /locus_tag="ACHLAM_000782"
     tRNA            complement(3633..3708)
                     /locus_tag="ACHLAM_000782"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3673..3675),aa:His,seq:gtg)
     gene            complement(3767..3843)
                     /locus_tag="ACHLAM_000783"
     tRNA            complement(3767..3843)
                     /locus_tag="ACHLAM_000783"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3807..3809),aa:Arg,seq:ccg)
     gene            complement(3946..5331)
                     /gene="yifK"
                     /locus_tag="ACHLAM_000784"
     CDS             complement(3946..5331)
                     /gene="yifK"
                     /locus_tag="ACHLAM_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7852355.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5522..6262)
                     /gene="wecG"
                     /locus_tag="ACHLAM_000785"
     CDS             complement(5522..6262)
                     /gene="wecG"
                     /locus_tag="ACHLAM_000785"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO7852356.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6265..7617)
                     /gene="wzyE"
                     /locus_tag="ACHLAM_000786"
     CDS             complement(6265..7617)
                     /gene="wzyE"
                     /locus_tag="ACHLAM_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO7852357.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7614..8693)
                     /gene="rffT"
                     /locus_tag="ACHLAM_000787"
                     /gene_synonym="wecF"
     CDS             complement(7614..8693)
                     /gene="rffT"
                     /locus_tag="ACHLAM_000787"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO7852358.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8690..9940)
                     /gene="wzxE"
                     /locus_tag="ACHLAM_000788"
     CDS             complement(8690..9940)
                     /gene="wzxE"
                     /locus_tag="ACHLAM_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO7852359.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQELVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9942..11072)
                     /gene="rffA"
                     /locus_tag="ACHLAM_000789"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9942..11072)
                     /gene="rffA"
                     /locus_tag="ACHLAM_000789"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO7852360.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11077..11751)
                     /gene="rffC"
                     /locus_tag="ACHLAM_000790"
                     /gene_synonym="wecD"
     CDS             complement(11077..11751)
                     /gene="rffC"
                     /locus_tag="ACHLAM_000790"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO7852361.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11729..12610)
                     /gene="rfbA"
                     /locus_tag="ACHLAM_000791"
     CDS             complement(11729..12610)
                     /gene="rfbA"
                     /locus_tag="ACHLAM_000791"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO7852362.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12629..13696)
                     /gene="rffG"
                     /locus_tag="ACHLAM_000792"
     CDS             complement(12629..13696)
                     /gene="rffG"
                     /locus_tag="ACHLAM_000792"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO7852363.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SVSKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13696..14958)
                     /gene="wecC"
                     /locus_tag="ACHLAM_000793"
     CDS             complement(13696..14958)
                     /gene="wecC"
                     /locus_tag="ACHLAM_000793"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO7852364.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14955..16085)
                     /gene="wecB"
                     /locus_tag="ACHLAM_000794"
     CDS             complement(14955..16085)
                     /gene="wecB"
                     /locus_tag="ACHLAM_000794"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO7852365.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16141..17187)
                     /gene="wzzE"
                     /locus_tag="ACHLAM_000795"
     CDS             complement(16141..17187)
                     /gene="wzzE"
                     /locus_tag="ACHLAM_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO7852366.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYFRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17199..18302)
                     /gene="wecA"
                     /locus_tag="ACHLAM_000796"
     CDS             complement(17199..18302)
                     /gene="wecA"
                     /locus_tag="ACHLAM_000796"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO7852367.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18542..19801)
                     /gene="rho"
                     /locus_tag="ACHLAM_000797"
     CDS             complement(18542..19801)
                     /gene="rho"
                     /locus_tag="ACHLAM_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO7852368.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19886..19987)
                     /gene="rhoL"
                     /locus_tag="ACHLAM_000798"
     CDS             complement(19886..19987)
                     /gene="rhoL"
                     /locus_tag="ACHLAM_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO7852369.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20128..20457)
                     /gene="trxA"
                     /locus_tag="ACHLAM_000799"
     CDS             complement(20128..20457)
                     /gene="trxA"
                     /locus_tag="ACHLAM_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO7852370.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20588..21853
                     /gene="rhlB"
                     /locus_tag="ACHLAM_000800"
     CDS             20588..21853
                     /gene="rhlB"
                     /locus_tag="ACHLAM_000800"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO7852371.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21987..23471
                     /gene="gppA"
                     /locus_tag="ACHLAM_000801"
     CDS             21987..23471
                     /gene="gppA"
                     /locus_tag="ACHLAM_000801"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO7852372.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMALAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23518..25539)
                     /gene="rep"
                     /locus_tag="ACHLAM_000802"
     CDS             complement(23518..25539)
                     /gene="rep"
                     /locus_tag="ACHLAM_000802"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO7852373.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25626..25907
                     /gene="ppiC"
                     /locus_tag="ACHLAM_000803"
     CDS             25626..25907
                     /gene="ppiC"
                     /locus_tag="ACHLAM_000803"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO7852374.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25953..27428)
                     /gene="ilvC"
                     /locus_tag="ACHLAM_000804"
     CDS             complement(25953..27428)
                     /gene="ilvC"
                     /locus_tag="ACHLAM_000804"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO7852375.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETVTEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27578..28468
                     /gene="ilvY"
                     /locus_tag="ACHLAM_000805"
     CDS             27578..28468
                     /gene="ilvY"
                     /locus_tag="ACHLAM_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO7852376.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28465..30009)
                     /gene="ilvA"
                     /locus_tag="ACHLAM_000806"
     CDS             complement(28465..30009)
                     /gene="ilvA"
                     /locus_tag="ACHLAM_000806"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO7852377.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30012..31862)
                     /gene="ilvD"
                     /locus_tag="ACHLAM_000807"
     CDS             complement(30012..31862)
                     /gene="ilvD"
                     /locus_tag="ACHLAM_000807"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO7852378.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31927..32856)
                     /gene="ilvE"
                     /locus_tag="ACHLAM_000808"
     CDS             complement(31927..32856)
                     /gene="ilvE"
                     /locus_tag="ACHLAM_000808"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO7852379.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32876..33139)
                     /gene="ilvM"
                     /locus_tag="ACHLAM_000809"
     CDS             complement(32876..33139)
                     /gene="ilvM"
                     /locus_tag="ACHLAM_000809"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO7852380.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33136..34782)
                     /gene="ilvG"
                     /locus_tag="ACHLAM_000810"
     CDS             complement(33136..34782)
                     /gene="ilvG"
                     /locus_tag="ACHLAM_000810"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO7852381.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34785..34835)
                     /gene="ilvX"
                     /locus_tag="ACHLAM_000811"
     CDS             complement(34785..34835)
                     /gene="ilvX"
                     /locus_tag="ACHLAM_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO7852382.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34922..35020)
                     /gene="ilvL"
                     /locus_tag="ACHLAM_000812"
     CDS             complement(34922..35020)
                     /gene="ilvL"
                     /locus_tag="ACHLAM_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO7852383.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35373..36893
                     /gene="yifB"
                     /locus_tag="ACHLAM_000813"
     CDS             35373..36893
                     /gene="yifB"
                     /locus_tag="ACHLAM_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO7852384.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36919..37257)
                     /gene="maoP"
                     /locus_tag="ACHLAM_000814"
     CDS             complement(36919..37257)
                     /gene="maoP"
                     /locus_tag="ACHLAM_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO7852385.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37376..38215
                     /gene="hdfR"
                     /locus_tag="ACHLAM_000815"
     CDS             37376..38215
                     /gene="hdfR"
                     /locus_tag="ACHLAM_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO7852386.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38310..38385)
                     /locus_tag="ACHLAM_000816"
     tRNA            complement(38310..38385)
                     /locus_tag="ACHLAM_000816"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38350..38352),aa:Trp,seq:cca)
BASE COUNT         9416 a        10486 c         9697 g         8824 t
ORIGIN      
        1 caacgcgata taacatcacc gccttacata tcacctgcgc cagaggtagg attgaaaacg
       61 ctctcctgat tttccaattc attttctaga taaataaata atttattttt atcactatta
      121 tttatgcaat catcctgtca gggagaggga tctcaattat caatgcttaa ttacgtcatc
      181 attttgacga catgaaaccg gagcattttg catggaattt tcgttttcac ccaaacttct
      241 tgttgtggct gtcgccgccg ctcttccact catggccaac gcagcagata ccccgtcaac
      301 cgccaccgca cgcaaaggct ttgccggata cgatcaccca aaccagtatc tggttaaacc
      361 ggcgaccact attgccgaca acatgatgcc ggtgatgcag catcctgcac aagacaaaga
      421 aacccagcag aagctggcag aacttgagaa aaaaaccggt aagaaaccga acgtggttgt
      481 tttcttgctg gatgatgtgg gctggatgga tgttggcttt aacggcggcg gcgtggcggt
      541 gggtaaccct acaccagata tcgacgccgt tgccagccag gggctgattt taacttcggc
      601 gtattctcaa ccgagttctt ccccaacccg cgccacgatt cttaccggac aatactccat
      661 ccaccacggc attctgatgc cgccaatgta cgggcaaccg ggcggtctgc aagggttaac
      721 cacgctgccg cagttgctgc acgatcaggg ctacgtcact caggccatcg ggaaatggca
      781 tatgggggaa aacaaagagt cgcagccgca gaacgttggc tttgatgatt tccgcggctt
      841 taactcggta tccgatatgt ataccgaatg gcgtgatgtt cacgtcaacc cggaagtcgc
      901 cctgagcccg gatcgttctg aatacatcca aaaactgccg ttcagcaaag atgacgttca
      961 tgccgtgcgc ggcggtgaac aggaagccat tgccgacatt acgccgaaat atatggaaga
     1021 cctggatcaa cgctggatgg aatatggcgt taagttcctc gacaagatgg cgaagagcga
     1081 taagcctttc ttcctctact acggcactcg cggctgtcac ttcgataact acccaaatgc
     1141 gaaatatgcg ggtagctctc cggcgcgcac ctcttacggc gactgcatgg tggagatgaa
     1201 cgatatcttc gccaatctgt ataaagcact ggagaaaaac ggtcagcttg ataacacgct
     1261 gattgtgttc acctctgata acggtccgga agccgaagtt ccgccgcacg gtcgcacacc
     1321 gttccgcggt gcgaaaggtt ctacatggga aggcggcgtt cgtgtgccaa ccttcgttta
     1381 ctggaaaggc atgatccaac cgcgtaaatc tgacggcatt gtcgatctgg cggatctctt
     1441 ccctacggcg ctggatctgg cggggcatcc tggggcgaaa gtagcgaatt tagtaccgaa
     1501 aaccaccttt atcgatggtg tggaccagac atctttcttc ctgggaacaa atggtcagtc
     1561 taaccgtaag gccgagcact acttcctcaa cggtaaactt tctgctgtgc gtatggatga
     1621 gttcaagtat cacgtcctga ttcagcaacc ttacgcttat acccagagcg gatatcaggg
     1681 tggattcacc ggcacagtaa tgcaaacggc gggatcgtcg gtgtttaacc tgtacaccga
     1741 tccgcaggaa agcgactcta tcggcgtgcg ccatattccg atgggtgtac cgctacagac
     1801 cgaaatgcac gcgtatatgg agatcctgaa aaaatatcca ccacgcgcgc agattaaatc
     1861 tgactaagcc ggcgctatcg ctgagatctg cctttgccgg atgcgatgct gacgcatctt
     1921 atccagccta cagaacgctg caatttattg aatttgcaat gattgtaggc cggataaggc
     1981 gttcacgccg catccggcaa tcaaccgcag gcggccgccg atttctactt attcaccacc
     2041 atcaaatgcg cctgcataat gtcgctggcc ggacgaccgt gcgccagcaa atcagccatt
     2101 gctttaagat atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc
     2161 agcccgggtt tgccgctggc atcgagcata aagcggtgtt tcgggcagcc tccccagcac
     2221 gcttttaaca cgttgcaact gcgacactgt gccggtaact gcttgaattt atcttcacca
     2281 aatgcctgct gttgcgggga atcaatcatt tctgcaatcg tctgctgaag catattccct
     2341 agtcgatact gcggataaac atagtgatcg caggcgtaaa catcgccgtt gtgttcaaca
     2401 atcaccgagc gcccacaggt tggctgatga tggcaaaccg cgcccggtgc gccgacaaaa
     2461 ttggcgaacg cccattcgat attcattacg aaaaccttgc cgacgtcgcg cttaatccag
     2521 tggtcgaaaa tcgccaccag aaactcaccg aactcttgcg ggcacaccga ccattccgtc
     2581 agttcgccct gaatatcgcc gggagcatgt agcttaagtc cagcatgagc agccgtttca
     2641 tcagccaggc gctcgaccac cggaataaac tggatgaact caacacctgc atcacacaga
     2701 aaatcataga cttgcaaagg ttgtaacgca ctggtgcgat ttacgcagac cagcacgtta
     2761 tagtcgacat gatgtttttg caggagcgtc agggcacgca tcaccagctt atgcgttggt
     2821 ctgccgcctt tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac
     2881 ccaacaagaa aatgattttc tgccagaaat gcacaccatt cgtcatcaag cagcacgccg
     2941 ttagtctgga agctgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg
     3001 gcacggcggt aaaaatccag cccgagtaac gttggttcgc cgccctgcca ggtaaaagcc
     3061 acttcgtttt gggtttcgct ggcagcgata tagtgacgga catacgcttc cagcgtgtca
     3121 tcgtccatat gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag
     3181 tagtcacagt tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc
     3241 gttggaacct gttgcagcat ggtcgctcct tgcctgaagt catccttcaa taagttaagg
     3301 gcgaaatcat accgtggcgc aatgcgttat tacgggcgtt tattgcgaag ggaaagtgac
     3361 gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga
     3421 ggattcgaac ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg
     3481 ccgaaatact gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg
     3541 gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc
     3601 cgccaccttc gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac
     3661 gacaactgga atcacaatcc agggctctac caactgagct atagccacca ctacaaatct
     3721 tgttacgcgg tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca
     3781 ggattcgaac ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg
     3841 cgcttagcgc cgttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt
     3901 tttaaataaa ttgcgcgttt ggttacggtt tgtgcgcttt gctgcttatt tctccagttt
     3961 atgcgcttta ccgtggcgat taagcccaaa aactttataa atcgccgtca ccgtcagcat
     4021 aaagatgatg ccgacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa
     4081 aacgcaaatc aggaatgcca ttgttacgta gttggcccac gggaacagga ttgagcggaa
     4141 cggatggctg gcaattgccg ctttatgcgc acgacgaaaa cgcagctggc taatcaatat
     4201 cacaaaccat ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg
     4261 ctgcggattg ggaatgatgt agttcaggca tgagccaatt aacaggatag caatagatac
     4321 tgccacaccc gcaaccggta cgccgtgacg ggaaactttc gccatcgccg tcggtaactg
     4381 acggtttttc gccaatgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc
     4441 agagagcgca gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taatgccgat
     4501 tttggcgaaa gtcagtacga acgggctgcc gttactgcct atttcattcc acgggaagat
     4561 ggtgacgata acgaaaatcg cgcctacgta gaaaatcagg atacgccaca acactttgcc
     4621 tacggcactg cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag
     4681 ctccacgccc tggtaggacg ccaccacaat acacaatgcg gtcaggaacc ctttccagcc
     4741 acccgcaaag aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt
     4801 gccaaaacca aagaaaatca cgcccaagcc aatgacaatc atcacgataa tcgtggtgac
     4861 tttgatcatc gcgaaccaga actcgatttc gccgtacaac cgcactgccg ccagattcgc
     4921 cagcgccacc agcgccaccg cgatcaatgc gggtatccac tgcgccatct ccgggaacca
     4981 gaactggaca taaacgccaa tggcggtgat ttctgagatc cccaccgcca tccacataaa
     5041 ccagtaagac caggcggtga gatagccaaa aaacgggctc atataacgat gcgcataaac
     5101 ggcgaacgaa ccggtaaccg gttcgaggaa caacatttcg cccattgaac gcatgatgaa
     5161 aaagacgaac agcccggcga tgatataggc caacaatacg gatggcccgg cccatttcag
     5221 ggtactggcg gcccccataa acaggccgac gccaatggtg ccccccaggg cgatgagttc
     5281 gatatgtcga gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg
     5341 tgttgtgttt gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc
     5401 gaatgttttc gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt
     5461 gtaataaaaa agcagacagg cgacggagtg accactccgt cgctttacaa agagaggaaa
     5521 atcagaggtt gccggtgtag tgccagcgta aataacgcag caaacgaagc tgacgcttaa
     5581 tgcggctcgg ctgcgaaagc aggcggtaga gccactccag ccccagcgtt tgccagattt
     5641 tcggtgcgcg ttttacgtga ccggtgaaaa catcgtaggt cccgccaacg cccatataca
     5701 gcgcatctgg atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca
     5761 tcgcaacggt gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct
     5821 gctcgggttt aaaataacca tcctgactgc caacgatatt cacattccac tggttgcgca
     5881 gtttagcttc agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc
     5941 cttctttgcc tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa
     6001 cctgcgcctg cgggtacttt ttacgtactg aacgtacaac gctgatgcca tccgcatatt
     6061 taaattcggc agcgttaatt agctccctga cctcagcatt atcttcaatg gtcagcattt
     6121 tttcggcatt aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat
     6181 cgagggcgtg ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg
     6241 gtgccgtggt gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt
     6301 acgtttatga atgagtccgg cgctttcaaa aagccagtac aacagttttg cgatcatcag
     6361 acatgcgccg aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc
     6421 acgtgccagc acgatcatat tgaagatcgc cccgaagcag aaactgtgca atatcgcagc
     6481 cttatagcga ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat
     6541 cagaccaacc acaatcgccc cgagcgggat gaacaacgcg ccgcccatca ccaccagtga
     6601 gcctataagc gtaggcgaga tcgccagccc ggagtggtta ttcagcactt cccaggtaaa
     6661 gtagttggcg gagttcagta ccatactcgg gcgacccggc cacagccagg aagggataaa
     6721 gacatagaaa tcgcggacaa tgggagccag gccctggaag tcgatgttgt cgtagttctg
     6781 caacagcaac gccagattct cccacggcga aaaggtatcg cgggtgagat agagaaacgt
     6841 atagaacgct tcatcgccgc tcacattcat tccataacgt tttagcgcca gccagaacat
     6901 gcccacaatc cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat
     6961 aatgccaata aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac
     7021 aatcatgtaa gtcaacaagc caaaggcaac cgtgctgacg aggaaaaaca gccacgcttt
     7081 gctatcctgg cgcagaaaat aaaccaccag catcgccggg atgaaaaagt aaaagaagcg
     7141 ttttaatgcc acgccggaga cttcgctgga aaagatctga ctgtaagaat taagccggaa
     7201 cagcaaaaag ccgttgtgca tgaagaagat gccgacgctc accagagcga tgcccatcag
     7261 gatcacccac gtgagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtgc
     7321 atcagcaacg cgtttgcgta gacgggtttt gtaggtgaca tagtaaaccg cgtagaagca
     7381 gcctgcagaa agcaacgcct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg
     7441 aaataccagc acgctggtca gcggaaagcc gaagaaaaag gtgagcaaaa acagcaatga
     7501 aaagaagaca ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat
     7561 aaacaacgtg cagagcagcc agacaacaaa caggccactg aattgcagca gactcatgcg
     7621 acctccccgg cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag
     7681 aaggcaatgg tgtttttatc caccgacgcc aactggcgct gcgcttcacg cacaatatcc
     7741 tcgttgagat catcggtagt aaacagtact ggcaaatgct gttccgtcat atcctgccag
     7801 aacggatttt cccggttaag cacacaagga atgcccgcct gaatcagtaa gcaaagcgta
     7861 ccaatgccct gctggcgggc aaaaataaag taaccgagat cgcactgacg aagtagcgtc
     7921 agataggcgt caaattccag tttttcgctc agaacttgca gattttcttc gctgaataac
     7981 tccagtcccg cctgacgaac ttcctcgatg tacgcttcgt tattaggcgg atatcccatc
     8041 ggcaccacca cttttaccgt atcgccaaat tgctgatgaa cggcgcgcaa ggcagcaata
     8101 tgctcattgc tgcggtcacc ggagttcccc accagaatgg tcatttttac ttcacgttgc
     8161 cgatcgttca ccatcgtatt aagagaagag tccatccgcg tcgggaagta cagcagttcg
     8221 ccccgcacct ttgggtgcgt tttggcaaaa aagctcaaat caccgcgggt ggcaaataca
     8281 cagccgactc gcttttgcgc caggcgacgt agtgggtaaa aaagcttata tctcaagcca
     8341 ctggaaagtt cgtacaggtc tgccccccag atatgccagt aaaactggct gggcttaatg
     8401 ccaccactca gcagagccag ccacagtgtg ggattgaact gaccgtggaa gaaaaaacgc
     8461 tgctgacggt tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg
     8521 aaaaattgca cagaaagcgc cagacagcta tcacttaagc cgtcgtcctt gccaacaacc
     8581 ataaactcgc gcgcgtgctc gctcgtcgcg gccagcgcgt cgttgaaaaa ccgcagaacg
     8641 gttcggttat ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc
     8701 tacgccagag taaaaacacg ccacaacaaa gagaaaaata gacgatatag gttgccatat
     8761 aggcctgcgc tgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca
     8821 ataaagtgaa ctggctgact tccgccagaa tataaaaccg cagtgacgct ttggcgatca
     8881 ccagataacc aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa
     8941 agagatcgcg catagcggta aatttattcg acaacagcag ccagatagca aaatcacgca
     9001 gcaaccagac ggtaaaactc gccgccgcca ctgccggtaa gacgaatttt agcgatttaa
     9061 ccacttcccg ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata
     9121 cgctgaacga tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc
     9181 agatccccac ctcatcccag ctatactgcg ccgccagcag tttacgcatc atgatgtaag
     9241 caacaggcaa ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg
     9301 ccagaccgtt atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttgatca
     9361 acatgatggc ggcaggaatt accaccagcg cgggaatcag cgccagaccc agcaacgccc
     9421 cttcataacc gcccaaacgg taactgacgt agtacgcaag aacgccaatc aagctgccaa
     9481 caatcagcga taacgcatta cctgcggcat cgcgaaagcc tttcatcaac gccagtaaca
     9541 ggttgcccca ggcgatcccc atttgcacca acgccactaa acgcaccagc tcctgatagt
     9601 cggtattacc aaacaatccc tggctgattg gcgcagctgc cagcacaaaa accagcgcca
     9661 tcagcgtaga gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt
     9721 ggggattatc atggtactgg gcaacgtatt tggttacgcc gttaaagatg cctgccccgg
     9781 caagcacgcc gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg
     9841 gaccaaatga cactgccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg
     9901 tactggccgc agtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac
     9961 aaggtcgcaa ttaccgtacg ctgattgacg ggcgacaggt tgtagaacaa cggcaggcgc
    10021 agcaggcgct cgctctcttt ggtggtgtag cgatcttcac cgtggaactc accaaagcgt
    10081 tcccccgcag ggcaaccgtg cagcggaatg taatggaaca ccgccatgat ttccgcttct
    10141 ttcagaaagt taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata
    10201 tgcgcgttct gcacgcagcc atcgggaatc gacggcagct cgatacgccc ggctttcgcc
    10261 agaggtgcta acgcatcgta gtagttttgc cacagcgcca gacgttgctg attgatacga
    10321 tccgctgctt ccagttgcgc ccacaggtac gcagcttgca aatcggacat caaatagctg
    10381 gagccgatat cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta
    10441 gtgccttttt cacggatgat ctcggctcgt tcgatcaacg ctttatcgtt aatcagcgtc
    10501 gcaccgcctt caccgcccgc cgtgtagttt ttggtttcat ggaagctaaa gcagccaata
    10561 tgaccaatgg ttcccagtgc acgccctttg taagtggaca taacgccctg agcggcatct
    10621 tccaccacaa acagattata ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc
    10681 acacccgcgt aatggaccgg cacgataacg cgcgttttgt cggtgatcgc cgcttcaatc
    10741 agcgtttcgt cgatgttcat agtgtccggg cgaacatcca caaagacgat tttcgcgcca
    10801 cgcagcacaa aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg
    10861 ccaggctgga tatcgagcag cagcgccgcc atctccagag aagcggtgca ggacggcgtc
    10921 aacagcactt tggcgctgcc aaaacgttgt tccagccact gctggcagcg acgggtaaaa
    10981 ccgccatcgc cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt
    11041 cccaccaccg gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt
    11101 gctttctaca ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatctg
    11161 ggtcgccacc cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ctgtttgcat
    11221 cagctcagca cctgcaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag
    11281 ttcccgtaaa gagacatagc cgcgaatatc gccggacgcc gaacgtaaaa tgaggcattg
    11341 atgatcaaag gtgccgcgca cggcattttc aatccactgt gcataaaagc gaccgctggc
    11401 gtcaggcgca taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tggctaactg
    11461 acgtaatgcg ggaatatcgg tctcttgtgc cactacagca ccactatcac tgacattgtt
    11521 tacgggtagc gccaaatcaa cttcaccttc taccagggag aatcccagct gttgcaaggc
    11581 atccagttca tccgtatttg atgccgcaat tttggcctgc acccgtgacc acggcgctaa
    11641 ggcgtctggc gtcaggagcg gtgcttcagg actaatgcgc acaatggcgc tgttaacacc
    11701 aaagaaggcg ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact
    11761 ccagcagata ttggccgtag ccagttttcg ccagcgagct ggcggcgcgc ttcacgcctt
    11821 cgtcatcaag ccagccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct
    11881 ggcgtttttc caccgtctgc acaaaggtgc tggcttcaat caggctgtcg tgagtgccag
    11941 tgtccagcca ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt
    12001 acatctggtt gatggaggta atctccagtt caccacgctc cgacggcttc acctgctttg
    12061 cgtactccac gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg
    12121 gctgttttgg cttttcttcc agcgagatag cgcggaaatt gtcgtcaaac tccacgacgc
    12181 caaagcgttc cggatccatc acctgatagc caaataccgt cgcgccttgc gtgcgcgccg
    12241 caacatgacg cagcttcgga ctgaagccct gtccgaagaa gatgttatcg cccagcacca
    12301 gacaagaagg ttcaccgttg aggaaggttt caccgatgat aaaggcctgc gccagaccgt
    12361 ccggactggg ctgttcggca tattccagct ggataccgaa ctcactacca tcgcccagca
    12421 ggcgctggaa ataaccttta tcttccggcg tagtgatgat gagaatttcg cggataccgg
    12481 ccagcatcag caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt
    12541 gcttcgatac gccgcgcgta atcggatgca atcgggtgcc ggaaccgccc gccaggataa
    12601 tacctttcat gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcgccctgat
    12661 agctgccgtc ctgcacctgc ttccaccagc tttcattagc cagataccac tgaaccgttt
    12721 tacgcattcc actttcaaag gtttcctgcg gcagccagcc aagttcacgg gcaattttcg
    12781 aagcatcaat ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaggt
    12841 cacgatagtg cgccacgccg tgcggcttgt tcggagccag ttcttccagc agctcgcaaa
    12901 tggtttccac aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac
    12961 cgactttccc ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc
    13021 agtcacggat ttgctgcccg ttgccatata ccggcagcgg tttacccgcc agcgcgttga
    13081 ggatcatcag cgggatcagt ttttccggaa agtggtaagg gccgtagtta ttcgagcagt
    13141 tggtgataag cgtcggcaga ccataggtcc gcagccaggc gcgcaccaga tggtcgctgc
    13201 tggcttttga cacggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat
    13261 catccgtcga gtgcaggtca ccatatactt cgtcggtaga gatatgatga aaacggaacg
    13321 ctgatttttt atcttccgtc agcgcattcc agtaagcccg cgccgcttca agcaatgtat
    13381 aagtcccgac aatgttggtt tcaacaaacg ctgccgggcc gtcaatagaa cggtcaacat
    13441 ggctttcggc tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgcgcca
    13501 gttctgcccg atcgcagata tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg
    13561 gtgccagcga catcagattt ccggcgtagg tcagcttatc gaccactacc accgcgtcgc
    13621 tcgtttcgtt gatgatataa cgcaccagcg ccgagccaat aaacccggca ccacctgtta
    13681 tcagaatttt tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac
    13741 attgtcgcca ttgataactt tgaactgact atgatcgacc agcatcacca gcacgtctgc
    13801 cgttgccagc gcctcgtcaa gctgcgccag tgtacaaagc ccggtcagtt ttttcggcaa
    13861 ctggtggatg ttaggctcaa caaccagagt ttcgccgcta tgccactggg cgatcagttc
    13921 agcgatttcc atcgctgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc
    13981 aaagcaggcg attttcagtt cactggcgcg tttatcggta gcagcaaggc agtcggcaac
    14041 cgccgctttc acctgatcga taacccagaa cggtttgtga tcgttcactt cgcgcgcggt
    14101 acggataagc cgcgcctgct gcgggttctg tgccacgata aaccacggat caacagcaat
    14161 gcagtgaccg cccacgccag gaccaggctg aagaatatta acgcgagggt gacgattcgc
    14221 caggcgaatc agttcccaga cgttaattcc ctgatcggca caaatcagcg acagttcgtt
    14281 agcaaaagcg atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt
    14341 ccgcgagtta gtgacgacac actcaccttc gaggaaaatt ttgtacagtt cgctggcgcg
    14401 ggccgaacaa accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat
    14461 aacctggccc ggtaacacgc gttccgggca gtaagcaatg ttgacgtccg ccaactcgcc
    14521 cacctgctgt gggaaagtga gatctggacg catctctgct aaccattctg ccatcttctc
    14581 ggttgacccc accggcgagg tggattcaag gatcaccagc gcgccttttt tcagcactgg
    14641 cgcaatggag cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa
    14701 cggcgtgggt acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa
    14761 aaaaccgcct tctacggcag tttttactac gctcgccaaa tcaggttcga cgatatggat
    14821 ttcgccacga ttgatggtat caaccgcgtg ttggttgata tcgacaccaa tcacctgttt
    14881 ttgccgtgag gcaaacgcag ctgctgtagg cagcccgatg taacccagtc cgataacaga
    14941 aatggtcgca aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag
    15001 catgcctgac catcaccata cgggttatgg gcgcggctca tagcttgata ttcgttttcg
    15061 tcttttaaaa gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc
    15121 accgtacccg ccgtcaccgc ttccggacgc tcagtggtat cgcgcattac cagcaccggt
    15181 ttccccagcg aaggcgcttc ttcctgaatg ccgcctgagt cggtcaaaat cagccaggcg
    15241 tggttcatca gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc
    15301 acgtgcccca gaatgcgatt gaccggctct ctgacgttcg ggttgagatg cactggatag
    15361 acaatctgga tgtcctggtg cgtggtggcg atgtctgcca gcgcatggca gatttcttca
    15421 aagccacgac cgaagctctc acgcctgtga ccggtcacca gaatcatttt tttattgggg
    15481 tcgataaacg ggtaatttgc cgccagttct gaacgcagcg tgtcgctgct catcacctgg
    15541 tcacgcaccc ataacagtgc atcaatgact gtattaccag taatgaagat tcggctatcc
    15601 gcaacgtttt cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc
    15661 gccagatgcc cggtcaatgt acggttagcc tcttccggcc acggcgaata gagatcgccc
    15721 gtgcgcagac cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg
    15781 gttgccagcg tcgtcgtagt atcgccgtga accagcacga cgtctggttt gaactcggca
    15841 agaataggtt ttagcccttc cagaatccga caggttatct ctgtaaggcc ctgtcctggc
    15901 tgcattatgt tgagatcgta gtcaggtaca atggaaaaga gtttcagcac ctgatcgagc
    15961 atctcccgat gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac
    16021 gcatgcacca acggcgccat cttgatggct tccgggcgcg taccaaatac agtcagtact
    16081 ttcacatcga ttctcttcga ataagcggcg agcgcctttg cgctcaccgc agcagtgttg
    16141 ctatttcgag caacggcggg ttaatgcgac accagccccg atcagtcccc cgacaatgcc
    16201 ccacataatc atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt
    16261 acgaaaatag cgataggtct gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt
    16321 taacatggcc cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt
    16381 ttccagtcga gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc
    16441 ctcggcaggc acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc
    16501 ctgttcaata ctgttcatcc ggcgatcgta gatggctttc gccacctctt cctgacgctt
    16561 cacctgagct ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct catcattcag
    16621 atggctggct gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc
    16681 aggcgcagtt tcggcaatca gcttcacgct gtcattgacc gcgcgggtga agtcaccggg
    16741 aataaacacg atgttgttga tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc
    16801 caccatccgc tgtttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga
    16861 ggccagttgc ataacaaact ctttataagc ttcgtccatg accgatggtt ggtcggcaga
    16921 agccatgttt gaacggacat ccaggttacg caaaaattgc tgctgtgagt aatatccccc
    16981 cagcatattc accgttggac gatcggtaat cgctgtcgag ctccactcct ggcgagcaaa
    17041 aaaagtatac gccagcgcga ttaacgcaaa cgccagcccc atgccaataa tccacagctt
    17101 cccagcccac aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc
    17161 cggtttccca ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg
    17221 ccaccacgat ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag
    17281 gcacgcttga tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc
    17341 cactccggga caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgctgcg
    17401 gcaagggtaa tcagcacaaa cgcctggcgg gaagtaaacc cggcacgcat gatcaaatgg
    17461 tgaatatgct gacggtctgg agagaatggg ctcatgcctt tacgcaggcg acggtacata
    17521 atcgccacca tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg
    17581 ggatgagttt tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc
    17641 gtactgcccg catcccccat aaagactttg tagcggcgac ccaggatacc aaggttaagc
    17701 atgatgtatg gcaggatggc ggcgatcatc gcaaagcacc agattgcgag actggtttgc
    17761 ccgtcgaacc acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccacccagc
    17821 aagccatcaa tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc
    17881 gtcaggaagt aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacctagg
    17941 ctactgagat aaagtttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg
    18001 gcacggattt ttacgctgat atcaaaacgg tcatccagcg cgccaatgaa aacaagcaca
    18061 ccagcacaag cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg
    18121 aagcaaatcc ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt
    18181 ttgcggaagt taggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac
    18241 agaaacagtg tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc
    18301 acaatgcatg ctctcagaga aaattattag cagaagtata accacgaaga cctttattca
    18361 gaagggaaag tccgttccga acccaccagt cctgtgcaaa aattaaccat ttgatatgca
    18421 aattgcataa cacaccagca aaatccagga attttcctaa cattgccagc gcggcattaa
    18481 gattacagat ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc ataagacaaa
    18541 tttatgagcg tttcatcatt tcgaagaaat cgtcattggt cttggtcatt gccagtttat
    18601 taatgaggaa ttccattgca tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc
    18661 acattttctg cagttcttcc tgagtagtga gcagctcttc tttacgggta ccggaacggt
    18721 tgtagtcgat tgccgggaag acgcgttttt cagcgatctt acgagagagg tgcagttcca
    18781 tgttgcctgt acctttaaac tcttcgtaga taacttcgtc catcttggaa ccggtatcga
    18841 taagcgcggt agcgatgatg gtcaggctgc cgccctcttc cacgttacgc gccgcaccga
    18901 agaagcgttt cggacgatgc agggcgttgg catccacacc accggtcagc actttacctg
    18961 acgccggaac aacggtgttg taagcgcgcg ccagacgagt gatggagtcg agcaggatga
    19021 taacgtcttt cttgtgctca acaaggcgtt tcgccttctc gatcaccatt tccgcgacct
    19081 gaacgtggcg agatgctggt tcgtcaaagg tagaagcaac aacttcacct tttaccagac
    19141 gctgcatctc ggttacttct tccggacgtt cgtcgatcag cagaaccatc agcacacaat
    19201 ccgggtggtt gtaagcaatg ctctgagcaa tgttctgcag cagcatggtt ttaccggctt
    19261 tcggcggtgc cacaatcaga ccacgctgac cacgaccgat aggtgatgcc agatccagta
    19321 cgcgagcggt taaatcttca gtagaaccgt taccacgttc catacgcaga cgagagtttg
    19381 cgtgcagcgg ggttaagttc tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga
    19441 agttaacttc gttaactttc agcagcgcaa aatagcgttc accttctttc ggcgggcgaa
    19501 tcttaccaga gatggtatca ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa
    19561 cgtagatgtc atcaggaccg gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc
    19621 catcctgcaa tatctccagt acaccatcac caaagatatc ttcgccactc tttgcgtgct
    19681 gcttcaggat ggcaaaaata atgtcctgct tacgcatacg agccaggttt tccagcccca
    19741 tattttcgcc gagagtgatc agctcagaaa ccggcgtatt ctttaattcg gtaagattca
    19801 tagtggtgtg agttcttaaa cttggggtaa atctcgaact taatgttgtg aatggtatgg
    19861 caggatcatc catgcctgtt tacggtcatc gactcatgtc ttttcgctgc ctggtcacag
    19921 gagagtacgc agaactgaaa cgacaagacg gattgagtga cgagccagaa atctgttcac
    19981 ttcgcattta agataaaacg ggaagcgttg gaaattacaa gattcaaact taataaggta
    20041 tgtttaatac gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc agtttttagc
    20101 gacggggcac ccgaacatga aattccctta cgccaggtta gcgtcgagga actctttcaa
    20161 ctgaccttta gacagtgcgc ccactttggt tgccgccact tcaccgtttt tgaacagcag
    20221 caaagtcggg ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt
    20281 cagttttgca acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc
    20341 gatcattttg cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc
    20401 tttgagtaca tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatata
    20461 taactccaca ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact
    20521 aaaggttgac tttatttcac cggatacgct ttcgtaaagc aatagtaagc tgatattcta
    20581 ccacactatg agcaaaacac atttaacaga acagaagttt tccgacttcg ccctgcatcc
    20641 gaaggttgta gaagcccttg aaaaaaaagg gtttcataac tgtacgccca ttcaggcact
    20701 ggcccttccg ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg
    20761 gaaaacgatg gcgtttctta cgtcaacgtt tcattatctt ctctctcatc ccgcgattgc
    20821 cgatcgcaag gtgaatcaac cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt
    20881 gcagatccac gccgacgcgg agccacttgc gcaagctacc ggcctgaagc tgggtctggc
    20941 ttacggtggt gatggctacg acaaacagct gaaagtgctg gaaagcggcg ttgacattct
    21001 gattggcacc acggggcgtt taattgacta cgccaagcag aaccacatta acctcggtgc
    21061 cattcaggta gtggtactgg acgaagccga tcgtatgtac gatctgggct ttattaaaga
    21121 tatccgctgg ctgttccgcc gtatgccgcc tgcaaaccag cgcctcaaca tgctgttctc
    21181 cgccacgctt tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca atgccgaata
    21241 tattgaagtg gaaccggaac agaaaacggg ccaccgtatt aaagaagagc ttttctaccc
    21301 ttctaacgaa gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt ggccagacag
    21361 agcgattatt ttcgccaaca ccaaacaccg ttgtgaagag atctggggcc acctggcggc
    21421 agatggtcat cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat
    21481 tcttgatgaa tttacccgtg gtgatttgga tattctggtt gccaccgatg ttgccgcgcg
    21541 tggcctgcat atcccggctg tgacgcacgt ctttaactac gatttacccg atgactgtga
    21601 agattacgtt caccgtattg gtcgtacagg tcgcgctggc gcaagtggtc attccatcag
    21661 cctggcatgt gaagagtatg cattgaattt gcctgctatt gagacctata ttggtcactc
    21721 aattccggta agcaaataca atccggacgc attgatgacc gatctgccaa aaccgctgcg
    21781 cctcacgcgc ccgcgtacag gcaatggtcc gcgtcgtact ggtgctccgc gtaatcgtcg
    21841 tcgttcaggt taaataaaat gtcatactca aaatcattca aactgtatca agatggcaag
    21901 agaatgaatc ccgatgcgca tgctgtagta agtgatttgg gtgaaagaac gcagccaaca
    21961 cagagacaga ttgaaggatg aagagtatgg gttccacctc gtcgctgtat gcagccattg
    22021 atctcggttc gaatagtttt catatgctgg ttgtgcgcga ggtggctgga agcatccaga
    22081 cgctgacgcg aattaaacgc aaagtgcgtc tggctgctgg cctgaatagc gaaaatgccc
    22141 tgtctaatga agcaatggag cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc
    22201 aagatatccc tccctcgcaa attcgcgttg tcgctacggc gacattacga ctagccgtca
    22261 atgcgggtga ttttatcgcc aaagcacagg aaatccttgg ctgcccggta caagtcatca
    22321 gcggtgaaga agaagcccgc ctgatttatc agggggtggc ccacacaaca ggcggtgccg
    22381 atcagcgcct ggtggtggat ataggcggtg ccagtactga actggtaacc ggcacgggtg
    22441 cacaaaccac ctcgttgttc agcctgtcga tgggctgcgt cacctggctg gaacgctatt
    22501 ttgccgatcg taatctggcg caggaaaatt ttgatgctgc agaaaaagcg gcacgcgaag
    22561 tgttacgtcc ggttgcagat gaattacggt atcacggctg gaaagtgtgc gttggcgctt
    22621 ccggcaccgt gcaggcgtta caggaaatca tgatggcaca ggggatggat gaacgcatta
    22681 ccctggaaaa gttgcagcaa ctgaaacaac gcgccattca ttgcggtcgg ctggaagagc
    22741 tagagattga cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc
    22801 tgatagccat ttttaccgaa ctgaatattc agtgtatggc cctggcgggc ggtgcgctgc
    22861 gtgaaggcct ggtctacggt atgttgcatc ttaccgtcga gcaggatatt cgcagccgta
    22921 cgctgcgtaa tattcagcgc cgctttatga tcgatattga tcaggcacag cgcgtagcca
    22981 aagttgcggc taacttctac gatcaggtgg aaaatgaatg gcatcttgaa gcaataagcc
    23041 gcgatttgct catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac
    23101 aagcgccgca acacgctgct tatctggtac gtaatctgga tcttcccggt tttacccccg
    23161 cacagaaaaa actgctggcg acgctactgc tcaaccagac taatccggtc gatctctcat
    23221 cgctgcatca gcaaaatgcc gtaccaccgc gcgtcgcaga acaactctgc cgtttactcc
    23281 gcctggctat catttttgcc agccgtcgcc gtgacgatct cgtgccagag atgacattac
    23341 aggctaacca tgaactgttg accttgacgc ttccgcaagg ttggctaacc caacatccgc
    23401 tgggtaaaga gattattgat caggaaagcc agtggcagag ctatgtccac tggccgctgg
    23461 aagtgcatta atgagtaagt gccggatgcg atgctgacac atcttttccg gccttgatta
    23521 tttccctcgt tttgccgcca tcatcgcttt cagattcgcc agatggcttt gccctttctg
    23581 catccgttct tcggcgctga ccactttgcg ctcctgttcc caaatcaaat catcctgcgg
    23641 cagctccagc aggaagcggc tcggctccgg gcgcaccagt tctccgtact gacggcgttc
    23701 tttacacagc gtaaaggtca attccttctg ggcgcgggta atgccgacat aggccagccg
    23761 ccgctcctca tcgatattat cttcatcgat gctgctctgg tgcggcaaaa acccctcttc
    23821 cataccgacc atgtagacat aaggaaactc cagccctttc gacgcgtgga gagtcatcag
    23881 ttgcacctga tccagctctt cttcactctc accacgctcc atcatgtcgc gcaaagtaaa
    23941 gcgcgtcacc acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt ccagcatctc
    24001 cgtcatccag ctaaacagtt ggttgacgtt cttcatgcgc atttcggcgg ctttcgggct
    24061 gggcgatgtt tcgtacagcc aggattcata atccatgccg tggatcagat cacgcaccgc
    24121 ggcaatcggc tcccgctccg ccagacgctg gatttctgcc aaccagtgag tgaagcgggt
    24181 caatgcttca taaccacgtc cgctaagcgt ctgactcagg cccatatcaa agctggcggt
    24241 aaacatgctt ttattgcgcg tcatcgccca ttcacccaac tttttcagcg tcgccgggcc
    24301 aatctctcgc ttcggcgtgt taacgatacg caggaacgca ctatcatcgt ccgggttagt
    24361 cagcacgcgc agataagcca gcaagtcctt gatttcagga cgagagaaaa acgacgtacc
    24421 accagaaatt ttgtacggga tgcggttttg catcaggaat ttttcaaaca cccgcgactg
    24481 atggttaccg cgataaagaa tggcgtaatc tttgtactgc gttttattga cgaagtgatg
    24541 ggcaatcagc tcgccagtaa cgcgctcggc ttcatgttcc tcgttattcg cgcttaaaac
    24601 ttttagctct gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga cgtgcgggtt
    24661 attggcgatc aggatgttcg ccgctttcaa aatacgtccg gaagagcggt agttctgctc
    24721 aagcttaatc accttcagcg ccggaaaatc ctgactcagc agcaccaggt tttgcggacg
    24781 tgcgccgcgc caggagtaga tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg
    24841 gctgcccacc agcagtttca ccagctcata ctggctggtg ttggtgtcct gatattcgtc
    24901 caccagcagg tagcgaattt tgttctgcca gcgctcgcgg acttcttcat tgcgttgcaa
    24961 cagcaacgtt ggcaataaaa tcagatcatc gaaatcgaga acgttacagg ctttcaggtg
    25021 tgcatcatac agcccataac aatgggcaaa aatacggtcc cgctcgccct ttgcctctgc
    25081 cgccgcctgc gccggtgttt tgagatcgtt cttccagttg gagatggtcg aaattagctg
    25141 ttgcaggaga actttgtcat cttcaatcag cccctcggtc aactctttaa gcaaagcaag
    25201 ctgatcggta tcgtcaaaca gcgaaaagtt agccttcatc cccaacgccg cgtactcacg
    25261 cttaatgata tccagcccca gcgtgtgaaa ggtggagatc atcagcccac gcgcctcttt
    25321 acgccccagc gtctgcccga cacgctcttt catctcgcgt gccgccttat tggtaaaggt
    25381 caccgccgcg atatgtcgcg cctgataacc gcaaccgcgg atcagatggg cgattttatt
    25441 ggtgataaca cgagttttac cggaacccgc gcccgccagc accaggcagg ggccggtaac
    25501 gaattcgaca gcttgttgtt ggccggggtt tagacgcata ggaaaatcgc tcaatgaaag
    25561 tcgggagggg aaaataacag cgtggtagta tagccagcct catccttacc actcaaggca
    25621 cgatcatggc aaaaacagca gcagcactgc atatccttgt aaaagaagag aaactggctc
    25681 tggatcttct cgagcagatc aagaatggtg ccgatttcgg caagctggcg aagaaacact
    25741 ccatttgccc gtcaggcaaa cgcggcggtg atttgggtga attccgccag ggtcaaatgg
    25801 ttccggcgtt cgataaagtg gttttctctt gtccggtact ggagccaacc ggcccgctgc
    25861 atacccagtt cggttaccac atcattaagg tgctgtaccg taactaacag caaggccttc
    25921 tccgggagaa ggccttgaac tctgaatcgc tattaacccg caacagcaat acgtttcata
    25981 tccgtcatat agccgcgcag tttcttacct acctgctcaa tcgcatggct gcgaatcgct
    26041 tcatttacat cacgcagctg cgcgttatct accgcacctt ccggaatagc tttacccagg
    26101 tcgcccggtt gcagctctgc cataaacggt ttcagcagcg gcacacaagc gtaagagaac
    26161 agatagttac cgtactcggc agtatcggag ataaccacgt tcatttcgta cagacgctta
    26221 cgggcgatgg tgttggcaat cagcggcaat tcgtgcagtg attcatagta agcagattct
    26281 tcgatgatgc cggaatcaac catggtttca aacgccaact caacgcctgc ttttaccatc
    26341 gcgatcatca gtacgccttt atcgaagtac tcctgttcgc cgattttgcc ttcatactgc
    26401 ggcgcggttt cgaatgcggt tttgccagtc tcttcacgcc aggtcagcag tttcttatcg
    26461 tcgttggccc agtcagccat catgccggag gagaattcgc cggagatgat gtcgtccata
    26521 tgtttctgga acagcggcgc catgatctct ttcagttgct cagagagcgc gtaagcacgc
    26581 agtttcgccg ggttagaaag acggtccatc atcagggtga tgccgccctg tttcagcgct
    26641 tcggtgacgg tttcccaacc gaactgaatc agtttttctg cgtatgccgg atcggtgcct
    26701 tcttccacca gcttgtcgaa gcacagcaga gaaccagctt gcaacatacc gcacaggatg
    26761 gtttgctcgc ccatcaggtc agatttcact tccgcaacga aagaggattc cagcacgccc
    26821 gcacggtgac cgccggttgc agccgcccat gctttagcga tcgccatgcc ttcgcctttc
    26881 ggatcgtttt ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct
    26941 tcacgtactt cggtgccagg gcatttcggc gcaaccatta cgacggtgat gtctttacgg
    27001 atctgctcgc ccacttcgac gatgttgaaa ccgtgtgagt aacccagcgc cgcgccgtct
    27061 ttcatcagtg gctgtacggt gcgtactaca tcagagtgct gcttgtccgg cgtcaggtta
    27121 accaccagat ccgcctgcgg gatcagttct tcgtaagtac ccactttaaa accattttcg
    27181 gttgctttac gccaggatgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaagaa
    27241 atatccagac cagaatcacg catgttcagg ccctggttca gcccctgtgc gccacagccg
    27301 acgatgacta cttttttacc ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc
    27361 ataaagcgac atttgcccag ctgtgccagc tgctggcgca gattcagtgt attgaagtag
    27421 ttagccatgg tgattcctcg tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga
    27481 ttgaattcac tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat
    27541 tgcaatttct gcaacgtcaa cattgagggc tgcgccattg gatttacgcg atctgaaaac
    27601 cttcctgcat ctggcggaaa gccgccattt tggccgtagc gcgcgggcaa tgcacgttag
    27661 cccgtctacg ctttcgcgac aaatacagcg cctggaagaa gatctcggtc agccgctgtt
    27721 tgtgcgtgat aaccgcacgg taacgctgac ggaagcgggc gaagagctgc gcgttttcgc
    27781 ccagcaaacg ctgttgcagt atcagcaatt gcgccacacc atcgaccagc aagggccgtc
    27841 gctctctggc gaattacata tcttctgctc ggtgaccgct gcctacagcc atctgccgcc
    27901 gattctggat cgcttccgcg cggaacatcc gtcagtggag atcaaactct ctaccggtga
    27961 tgcggccgat gcgatggaaa aggtggtcac tggcgaagcg gatctggcga ttgccgggaa
    28021 accggaaacc ttgccgggcg cagtggcgtt ttcgatgctg gagaatctgg cggtggtgct
    28081 gattgctccc gcgctgccct gcccggtgcg taatcaggta tctgtcgaaa agcccgactg
    28141 gtctacagtg ccgtttatca tggccgatca gggaccggta cgtcgccgca ttgaactgtg
    28201 gtttcggcgt aacaaaatca gcaacccaat gatttacgcc acggttggcg ggcatgaagc
    28261 gatggtatcg atggtggcgc tcggctgtgg cgtggcgctg ttgccggaag tggtgctgga
    28321 taacagcccg gaaccggtgc gtaaccgcgt gatgatttta gagcgcagtg atgaaaaaac
    28381 gccgtttgag ctgggcgtgt gcgtacaaaa aaagcggctt catgagccgc tgattgaggc
    28441 gttctggaag attttgccga accactaacc cgccaaaaag aacctgaacg ccgggttatt
    28501 ggtttcgtcg tggcaatcgt agcccagttc attcagccgg gtttcaaaat ccggttcatg
    28561 atcgccaagc tcgaacgccg ccagtacgcg cccgtagtcg gtaccgtggc tgcgataatg
    28621 gaacagcgag atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
    28681 cgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgcgatg gacgcccgcc
    28741 aaccatatag cgcacatgca gcttcgccat ttcgtcgtcg gagagatcca ccacgctgta
    28801 gccgccatcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccac ggctcaggcg
    28861 cacgccgaca aaaatgcagg cgtttttggc atcagcaaag cggtagttga actcggtgac
    28921 cgagcgccca ccaagtaact ggcagaattt gaggaagctg cccttttctt ccggaatggt
    28981 caccgccagt aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
    29041 accgtgaaag ttcacgttgg caccggaaag aatatgcgcc agccgctcac cgcgaatgtt
    29101 gtgctgggcg atgtattttt tcatccctgc cagcgccagc gcgccagagg gttcagccac
    29161 cgcgcgtaca tcttcgaaca ggtctttcat cgccgcacag atggcatcgc tatcgacggt
    29221 gatgatgtcg tcaagatact cctggcacaa acggaaggtt tcatcgccga tgcgttttac
    29281 cgcgacgcct tcagcaaaca gccccacgcg gggcagatca acgggatgac ctgcatcaag
    29341 tgccgctttc aggcaggcgg aatcttccgc ttctacggcg atcactttga tttgtggcat
    29401 cagttgtttg atcagcaccg ccacgcccgc cgccagaccg ccgccgccga ccggaacaaa
    29461 tacgcggtcg agatgagcat cctgctggag cagttccagc gccagcgtac cctgaccagc
    29521 gatcaccatc ggatgatcga agggtggcac ccaggtgaag ccctgctgct gtgacagttc
    29581 gattgctttc gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccaaa
    29641 gccgcgcacc gcatcaactt tgatatcggc ggtggcggtt ggcatgacga tcagcgcctt
    29701 cacgcctaac cgtgcggaag aaaacgcgac gccctgcgcg tggttacccg cagaagcggt
    29761 aatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcaccacg
    29821 aagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatca cgttatcaag
    29881 acgcgacgac agtttttcca ttttctgtag cggcgtgacc tgcgccgctt cgtagaccgg
    29941 tgcgcgcagc accgctctta aatattcggc accttccggg gcaccggaca ggggttgtga
    30001 gtctgccatc attaaccccc cagtttcgat ttatcgcgca ccgcgccttt gtcggcgctg
    30061 gttgccaggc tggcgtaggc gcgcagggca aaggagacct gacgttcacg gtttttcggc
    30121 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
    30181 cttacctgta actgaatacc acggttcgga atgtcgatgg cgatcaggtc gccgtcttca
    30241 atcaggccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat agaaaggcca
    30301 gaggtaccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
    30361 gatttcagga agctggttgg gtagagcatt tcctgcatcc ccggaccgcc tttcgggcct
    30421 tcatagcgaa ttactaccac atctccggcg acaactttac cgccgagaat cgcttctacc
    30481 gcatcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcc
    30541 acgcccgcgg ttttaacgat gcagccgttt tccgcaaagt taccgtagag caccgccagg
    30601 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccattggc gcgatcgtcg
    30661 tccagcgtat cccaacggca atcttgcgag aatgcctggg tggtacggat acccgcaggg
    30721 ccggcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
    30781 agcgtttgcg gcaacgtcag gccgagcacg tttttcacat cacggttcag taaccccgcg
    30841 cgatccagtt cgccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
    30901 tatttctggg tgctcggcgc aactttacac agctgtggaa ccttgcggga aagcttatcg
    30961 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag atgaagtacg
    31021 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cgttttcaaa cgccgcctta
    31081 ctggcgatat tacgcggcag tgcactatcg tcgttttgct cgtaataacg tttggtcaat
    31141 tcaacaatgc gtttaccagc attgaggaac agctgcttac ggtccgcgtg ggttgccagc
    31201 aacgagccgt tgcccggctg cgacaggccc agtgcttcgg tcaggcagtt cattgagtta
    31261 gcagtaaaca tcccggagca ggaaccgcag gttgggcagg cggatcgttc aacctgatcg
    31321 ctctgggagt cagaaacttt cgggtctgca ccctggatca tcgcatcaac cagatcgagc
    31381 ttaatgatct gatcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
    31441 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
    31501 ttagagatgc agaccatggc gtcggcgcag tgagcgttga ccatatactc aacggaatca
    31561 gcgatcaatt cgcgagatgg cagtgaataa agcatccccc cgtggcccat ggcaatccca
    31621 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
    31681 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
    31741 ttcacaaccg cgataatcgg cttaccgaaa tcggcgtcgg tcattccggt ggcgcgccac
    31801 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
    31861 atactttatt tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt
    31921 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
    31981 gaggccgaag aaggcttgct gaatgcgttt ggtaaccggg ccgcaacggc cttcaccaac
    32041 ctgaataccg tctacactgc gcactggcgt gatttctgcc gcagtaccgg acataaacac
    32101 ttcatccgcc aggtacagtg attcgcgcga cagcacctgc tcacgcactt caattcccag
    32161 ctctttcgcc agtttgatga tggcgtcacg ggtaataccc ggcagcgcgg atgaggtgaa
    32221 cggtggggtg aacagcacac cgtctttcac ttcaaacagg ttttcgcctg cgccttcaga
    32281 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gtgcttcact
    32341 acccaccagc agggaagaga ggtagttacc accggctttt gccgcggttg ggatggtgtt
    32401 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
    32461 gcccagatac gccccccacg ggaaagcggc gataatcacg tcggttgagt atcccgctgg
    32521 cgggtttacg cccatgccaa catcaccaac gaagatcagc ggacggatat aggcgctggt
    32581 gagattgttt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
    32641 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
    32701 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc
    32761 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
    32821 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
    32881 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
    32941 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
    33001 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgaacagacg tggaaaccac
    33061 gatgacgcac cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat
    33121 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg caccaggcgg
    33181 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
    33241 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttatgggtga tgtgttggcc
    33301 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc
    33361 gctgtatcgt tcctggaaaa acagttgctg ccattgtcga accatcccta accgttggtt
    33421 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
    33481 attcatcatg aaagagccgt caccggagat acagacgaca gtatcgttcg gtcgcgcgac
    33541 ttgtgcgcca accgccgccg gtaaaccgaa acccatggtg cctaagccgc tggaggtgat
    33601 gaagttttcc gggcgagtgt ggacgatgtg ctgggcagcc cacatctggt gctgccccac
    33661 atctgtggtc acgatgcaat ccgcagattt acgatccgac agttgtttta acaacaacgg
    33721 cgcgtagata gcgtcaccag gatggtcgta acgccaggca tgttcatcac gcagttgcgc
    33781 gcagtgttgc tgccagtcat cgatatttaa cggctgctgt aatgctggta acagagcatt
    33841 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccggatcgat
    33901 atccatatgg ataacgctgg cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc
    33961 atcaaaacgt gcgcccacgg cgatcagtag atcgcactcc tgcactgcga agttcgccgc
    34021 tttggtgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
    34081 cagccctttc aacgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
    34141 cgaaactgcc tgcgccatgc ccacgccacc accaacgtac agcatcggtt tttgcgcttt
    34201 tgccagcatc tgacgcgctt gctcgacttc ggcatgtggg aaagtcactt cgttttcaac
    34261 agtggtgaac cacggttcca gatcgccgct ggctaactgg atatcttttg gaatatcgac
    34321 caaaaccgga ccaggacgac cagagctggc gacgtcaaac gcttcggcca tgatacgcgg
    34381 taactcttcc agtgattgca ccaggaaact gtgcttggtg caggctaacg acaaccccag
    34441 aatatccacc tcctgaaatg cgtcagtgcc gataaacggt gcggatacct gaccggtgat
    34501 agcaacaacg gggatggaat ctaacagcgc gtccgcaagc ccggttatca ggttggttgc
    34561 acctggacca gacgtggcga tgcaaacgcc cgttttgccg gtagcacggg cataaccgat
    34621 agccgccatt gctgcacctt gctcgtgtcg gcacagcaag tgttccacgc caccgtcata
    34681 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
    34741 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
    34801 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcagt tatgttttta
    34861 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
    34921 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
    34981 taatcacgac caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    35041 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atattttttg
    35101 gccgattttt atgatgtctt taataatttc aagagaaatt gttgtttttt aaaaagaaaa
    35161 tagtgagtga aaaaaattca atttcagtat gaatgttgtt cccttcctcg tagttcatcc
    35221 tttgcccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    35281 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    35341 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    35401 cagccctggg agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtctac
    35461 caggcttaac gatggtgggc ttaccagaaa caacggtaaa agaagctcgc gatcgcgtgc
    35521 gcagcgccat tatcaatagc ggatatgaat atccggcgaa aaaaatcacc atcaaccttg
    35581 ctccagccga tctgccaaaa gaagggggac gatatgattt gcctatcgct attgcgttac
    35641 tggcggcctc agaacagctt acagccaata agttagatga atatgaatta gtcggagaac
    35701 tggcgcttac aggcgctctg cgtggcgttc ccggcgcaat ctccagtgca actgaagcca
    35761 ttaagtcggg cagaaaaatt atcgtcgcga aagataacga ggatgaagtg gggctaatta
    35821 acggtgaagg atgcctgata gccgatcatc tgcaggctgt ctgtgcgttt ctggagggta
    35881 agcacgctct cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc
    35941 tcagtgatgt tgtcggtcag gagcaaggaa agcgaggact ggaaattacc gccgctggcg
    36001 ggcacaacct tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta
    36061 ttaatggcct gttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa
    36121 gtctggtaaa tgctgaatca gtacaaaaac aatggcggca gcgcccattc cgctcacctc
    36181 atcacagtgc gtcgttaact gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa
    36241 tttcgctggc gcataacggc gtgctttttc ttgatgagct acctgaattt gaacggcgta
    36301 cactggatgc cttgcgagag ccgattgaat ccgggcagat ccatctttca cgcacacgag
    36361 caaaaataac ctatccagcc cgtttccaac ttgttgcggc gatgaatccc agccctaccg
    36421 gacattatca gggaaaccat aaccgctgca caccagaaca gacattacgt tatctcaacc
    36481 ggctctcggg gccctttctc gaccgcttcg atctctcact agagatccca ttaccgcccc
    36541 ccggcatttt gagtaaaacg gtagtgcagg gagaaaacag caccaccgtt aaacaacgtg
    36601 taatggccgc cagagagcgc caatttaagc ggcagaataa actgaacgcc tggctggata
    36661 atccggaaat acgccaattc tgcaagcttg agagcgaaga tgcgcagtgg ctggaagaaa
    36721 cgctgatcca tctggggtta tcgattcgtg cctggcagcg gttattgaaa gttgcacgaa
    36781 ccattgctga tattgatcag tctgacatta tcacacgtca gcatttgcag gaggcagtta
    36841 gctatcgtgc gattgaccgt ttgctcatcc atctgcaaaa actactgaca taaaaaaaag
    36901 ggcatttcgc cctttttatt aatcgtcaga atcggtgtag tcttcagcac cttcaacctg
    36961 cggtttaccg ccggaaaggg tgtgaaaacg ttttggacgc ttgatacgcg tcatatactt
    37021 ggaccacacg cgttctgctt ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa
    37081 gagtttctct tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata
    37141 accatgacgc tcaagcagtt gtgcctcttt gatggtgaag tcaccatgac gagagaatcc
    37201 acgtggataa tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct
    37261 gcacgctcct aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca
    37321 gagtgtaaaa caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga
    37381 tacggaattg ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcagc
    37441 tgaatcgctc tatctgaccc aatcagccgt gagctttcga atcagacaac tggaaaatca
    37501 actgggtgtg aaccttttca cccgccacag aaacaatatc cgtttaaccg ctgctggtga
    37561 aaaactactg ccttatgcag aaacgctcat gagcacctgg caggccgccc gtaaggaggt
    37621 ggcgcatacc tcacgacata acgagttttc tatcggtgcc agcgcctcgt tgtgggaatg
    37681 tatgcttaat cagtggctgg gacgcttgta tcaaaatcag gatgtccata caggcttaca
    37741 gttcgaagcg cgaattgccc aacggcagtc tctggtaaaa cagctgcatg aacgccagct
    37801 tgatcttctt attactactg aagcgcccaa aatggacgaa tttagcagtc agttgctcgg
    37861 gtatttcact ttagcgcttt ataccagtgc cccttctaaa ctaaagggag atcttaacta
    37921 tctgcgactg gaatgggggc cagattttca acagcatgag gcaggtttga tcggtgctga
    37981 cgaagttccc attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgacgct
    38041 taatggttgc acctggctac ccgtcagttg ggcgcgtaaa aagggcggcc tgcataccgt
    38101 tgtcgatagc acaacacttt cacggccgct ttatgccata tggctgcaaa atagcgataa
    38161 aaatgcgttg attcgcgatc tattgaaaat taacgtgctg gatgaagtgt attaatctga
    38221 atggctggca aggatgccgg tagaaggatt tacttcggag aggttatttc agataaaaaa
    38281 aatccttagc tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac
    38341 acccggtttt ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga
    38401 acggacggga ctcgaacccg cga
//
LOCUS       DAZGAP010000018        37396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGAP010000018 DAZGAP010000000
VERSION     DAZGAP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.018"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            116..1489
                     /gene="aroP"
                     /locus_tag="ACHLAM_000817"
     CDS             116..1489
                     /gene="aroP"
                     /locus_tag="ACHLAM_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO7852387.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTCFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1532..2386)
                     /gene="ampE"
                     /locus_tag="ACHLAM_000818"
     CDS             complement(1532..2386)
                     /gene="ampE"
                     /locus_tag="ACHLAM_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO7852388.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVLVVIAL
                     LTIYGALV"
     gene            complement(2383..2934)
                     /gene="ampD"
                     /locus_tag="ACHLAM_000819"
     CDS             complement(2383..2934)
                     /gene="ampD"
                     /locus_tag="ACHLAM_000819"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO7852389.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3022..3915
                     /gene="nadC"
                     /locus_tag="ACHLAM_000820"
     CDS             3022..3915
                     /gene="nadC"
                     /locus_tag="ACHLAM_000820"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO7852390.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4118..4558
                     /gene="ppdD"
                     /locus_tag="ACHLAM_000821"
     CDS             4118..4558
                     /gene="ppdD"
                     /locus_tag="ACHLAM_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO7852391.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4568..5953
                     /gene="gspE"
                     /locus_tag="ACHLAM_000822"
     CDS             4568..5953
                     /gene="gspE"
                     /locus_tag="ACHLAM_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO7852392.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVENPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAINAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            5943..7145
                     /gene="hofC"
                     /locus_tag="ACHLAM_000823"
     CDS             5943..7145
                     /gene="hofC"
                     /locus_tag="ACHLAM_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO7852393.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALFPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTE
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7180..8223)
                     /gene="guaC"
                     /locus_tag="ACHLAM_000824"
     CDS             complement(7180..8223)
                     /gene="guaC"
                     /locus_tag="ACHLAM_000824"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO7852394.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSFEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8379..8423)
                     /locus_tag="ACHLAM_000825"
     CDS             complement(8379..8423)
                     /locus_tag="ACHLAM_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852395.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8448..9068
                     /gene="coaE"
                     /locus_tag="ACHLAM_000826"
     CDS             8448..9068
                     /gene="coaE"
                     /locus_tag="ACHLAM_000826"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO7852396.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9068..9811
                     /gene="zapD"
                     /locus_tag="ACHLAM_000827"
     CDS             9068..9811
                     /gene="zapD"
                     /locus_tag="ACHLAM_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO7852397.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9821..10018
                     /gene="yacG"
                     /locus_tag="ACHLAM_000828"
     CDS             9821..10018
                     /gene="yacG"
                     /locus_tag="ACHLAM_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO7852398.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10220..10618)
                     /gene="mutT"
                     /locus_tag="ACHLAM_000829"
     CDS             complement(10220..10618)
                     /gene="mutT"
                     /locus_tag="ACHLAM_000829"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO7852399.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHLSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10678..13383)
                     /gene="secA"
                     /locus_tag="ACHLAM_000830"
     CDS             complement(10678..13383)
                     /gene="secA"
                     /locus_tag="ACHLAM_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO7852400.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13445..13957)
                     /gene="secM"
                     /locus_tag="ACHLAM_000831"
     CDS             complement(13445..13957)
                     /gene="secM"
                     /locus_tag="ACHLAM_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO7852401.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14188..15105)
                     /gene="lpxC"
                     /locus_tag="ACHLAM_000832"
     CDS             complement(14188..15105)
                     /gene="lpxC"
                     /locus_tag="ACHLAM_000832"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO7852402.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15206..16357)
                     /gene="ftsZ"
                     /locus_tag="ACHLAM_000833"
     CDS             complement(15206..16357)
                     /gene="ftsZ"
                     /locus_tag="ACHLAM_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO7852403.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16418..17680)
                     /gene="ftsA"
                     /locus_tag="ACHLAM_000834"
     CDS             complement(16418..17680)
                     /gene="ftsA"
                     /locus_tag="ACHLAM_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO7852404.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17677..18507)
                     /gene="ftsQ"
                     /locus_tag="ACHLAM_000835"
     CDS             complement(17677..18507)
                     /gene="ftsQ"
                     /locus_tag="ACHLAM_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO7852405.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18509..19429)
                     /gene="ddlB"
                     /locus_tag="ACHLAM_000836"
     CDS             complement(18509..19429)
                     /gene="ddlB"
                     /locus_tag="ACHLAM_000836"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7852406.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDANPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19422..20897)
                     /gene="murC"
                     /locus_tag="ACHLAM_000837"
     CDS             complement(19422..20897)
                     /gene="murC"
                     /locus_tag="ACHLAM_000837"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO7852407.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20951..22018)
                     /gene="murG"
                     /locus_tag="ACHLAM_000838"
     CDS             complement(20951..22018)
                     /gene="murG"
                     /locus_tag="ACHLAM_000838"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO7852408.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22015..23259)
                     /gene="ftsW"
                     /locus_tag="ACHLAM_000839"
     CDS             complement(22015..23259)
                     /gene="ftsW"
                     /locus_tag="ACHLAM_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO7852409.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23259..24575)
                     /gene="murD"
                     /locus_tag="ACHLAM_000840"
     CDS             complement(23259..24575)
                     /gene="murD"
                     /locus_tag="ACHLAM_000840"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO7852410.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAVTGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24578..25660)
                     /gene="mraY"
                     /locus_tag="ACHLAM_000841"
     CDS             complement(24578..25660)
                     /gene="mraY"
                     /locus_tag="ACHLAM_000841"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO7852411.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25654..27012)
                     /gene="murF"
                     /locus_tag="ACHLAM_000842"
     CDS             complement(25654..27012)
                     /gene="murF"
                     /locus_tag="ACHLAM_000842"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO7852412.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27009..28496)
                     /gene="murE"
                     /locus_tag="ACHLAM_000843"
     CDS             complement(27009..28496)
                     /gene="murE"
                     /locus_tag="ACHLAM_000843"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO7852413.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28483..30249)
                     /gene="ftsI"
                     /locus_tag="ACHLAM_000844"
     CDS             complement(28483..30249)
                     /gene="ftsI"
                     /locus_tag="ACHLAM_000844"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO7852414.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30265..30630)
                     /gene="ftsL"
                     /locus_tag="ACHLAM_000845"
     CDS             complement(30265..30630)
                     /gene="ftsL"
                     /locus_tag="ACHLAM_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO7852415.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30627..31568)
                     /gene="rsmH"
                     /locus_tag="ACHLAM_000846"
     CDS             complement(30627..31568)
                     /gene="rsmH"
                     /locus_tag="ACHLAM_000846"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO7852416.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31570..32028)
                     /gene="mraZ"
                     /locus_tag="ACHLAM_000847"
     CDS             complement(31570..32028)
                     /gene="mraZ"
                     /locus_tag="ACHLAM_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO7852417.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32630..33634)
                     /gene="cra"
                     /locus_tag="ACHLAM_000848"
     CDS             complement(32630..33634)
                     /gene="cra"
                     /locus_tag="ACHLAM_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO7852418.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33716..33802)
                     /locus_tag="ACHLAM_000849"
     CDS             complement(33716..33802)
                     /locus_tag="ACHLAM_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO7852419.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33814..34305)
                     /gene="ilvN"
                     /locus_tag="ACHLAM_000850"
     CDS             complement(33814..34305)
                     /gene="ilvN"
                     /locus_tag="ACHLAM_000850"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7852420.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34308..36032)
                     /gene="ilvI"
                     /locus_tag="ACHLAM_000851"
     CDS             complement(34308..36032)
                     /gene="ilvI"
                     /locus_tag="ACHLAM_000851"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO7852421.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36151..36195
                     /locus_tag="ACHLAM_000852"
     CDS             36151..36195
                     /locus_tag="ACHLAM_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852422.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(36350..37294)
                     /gene="leuO"
                     /locus_tag="ACHLAM_000853"
     CDS             complement(36350..37294)
                     /gene="leuO"
                     /locus_tag="ACHLAM_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO7852423.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9009 a        10433 c         9138 g         8816 t
ORIGIN      
        1 gcattatcat aattcagtta catgaaacgt aaagaaatca atacaacaaa cggaattgca
       61 aacttacaca cgcatcactg cgtagatcaa aaaaacaatc accgcacgag gtttcatgat
      121 ggaaggtcaa cagcacggcg agcagctaaa gcgcggtctt aaaaaccgcc atattcagct
      181 tatcgcgctg ggtggcgcga tagggaccgg gctattcctg ggtagcgcct ccgtaataca
      241 gtccgcaggg ccagggatta tcctgggtta cgccattgct ggttttatcg cctttctgat
      301 catgcgtcag ctaggtgaaa tggtggtcga agaacctgtc gcaggctcct ttagccactt
      361 tgcttataaa tactggggca gttttgccgg tttcgcctct ggctggaact actgggtact
      421 gtacgtttta gttgccatgg ctgagctgac tgccgtgggt aaatacattc agttctggta
      481 tccggaaatc ccaacctggg tttctgccgc cgtgttcttt gtggtgatta acgccatcaa
      541 cctgaccaac gttaaagtgt ttggcgagat ggagttctgg tttgccatta tcaaagttat
      601 cgcggtggta gcgatgatca tcttcggcgg ctggctgcta ttcagcggca acggcggccc
      661 gcaggcgagc gttagcaacc tgtgggatca gggtggtttc ctgccgcacg gcttcaccgg
      721 gctggtgatg atgatggcga ttatcatgtt ctctttcggc ggtctggaac tggtggggat
      781 caccgcagca gaagctgata atccggagca aagtatcccg aaagcaacta accaggttat
      841 ctaccgcatc ctgattttct atattggttc gttagccgtt ctgctctcgc tgatgccatg
      901 gacccgcgtt actgctgata ccagtccgtt tgtactgatc ttccacgaat tgggcgatac
      961 ctttgtggct aatgcgctga acatcgtggt actgactgct gcgctctccg tgtacaacag
     1021 ctgcgtatat tgcaacagcc gtatgctgtt tggtctggca caacagggca acgcgccaaa
     1081 agcgctggcg tctgtcgaca aacgcggtgt accggttaac actattctgg tgtctgcgct
     1141 ggttacggca ttgtgcgtgc tgattaacta ccttgcaccg gaatccgcat tcggactgtt
     1201 aatggcactg gtggtatctg cactggtaat caactgggcg atgattagcc tggcgcatat
     1261 gaaattccgt cgcgccaagc aggaacaagg cgtggtaact tgcttccctg ctctgcttta
     1321 tccgctgggt aactggatct gcctgctgtt tatggcggcg gtactggtga ttatgctgat
     1381 gaccccagga atggcgattt cggtatacct gatcccggta tggctgatcg tgttaggtat
     1441 cggctatctg tttaaagaga aaaccgccaa agccgtaaaa gcgcattaat ctctctacgc
     1501 cctcacccgt gcagggtgag ggcaataatc tttacaccaa cgccccgtaa atcgtcagta
     1561 gcgcaattac caccaggacc acgaacgagg ttttcttcgc catggaaacc gctgccttcg
     1621 gcgtctccac cttatcgaca tgcggttcac gcgccagaga gaactgtgcc agacgtgtta
     1681 atacctgata ttgcgaagta tggaaatcac ccagcgaagc aaaccaggcc ggtaacgctt
     1741 tctccccatg accgatcaag gcatatacca cacccgcaag acgaaccggc acccaatcca
     1801 gtacatgaag cacggcatca atgccggact gtaaacgatg atgcggcgtc tggtaacgcg
     1861 ccagccagta ttgccatgca cgcaaaaacg cataccccat cagcgtaacg ggtccccagg
     1921 ttccccccac aatcagccag aacagcggtg caagataaaa acgaaagtta atccacagca
     1981 atgcattttg cagctcacgc aaatactcac gttcatcgca gcctgccggg acgccgtgaa
     2041 tcatggtgag ttcgccagcc atcgtggcac gggcatggct atcattacgt gaagcagctg
     2101 tcagataagc atgataatga agacgaactt tacctgcgcc aatacacagc aaaccaatca
     2161 gcagccacac cagcagcgtg ggaacgttga acaatactcc ctgcaatgcg cgtaacagta
     2221 aaaaagtcac gcccatcgca ataatggtca tgcctaatgt gcgcccgaga gaaaaatgtt
     2281 tcacccgccg aaagaacgct tcaagacgat gatcaatctg ccagtgctcg cccaacttaa
     2341 acaggcgctc gaaaattaac accagtaagg ttgtaaatag cgtcatgttg tctccttgct
     2401 gaccagtgca cgaaaccgtg cccaatcaaa tgcagggccg ggatcggttt tccgatccgg
     2461 cgcaatatca caatggcccg tcatgttatt agcgatatct ggatagcgat caatcagtgc
     2521 ccgcgtaacc gccacaagct gttgatactg cgcatcggta tacgccagcg tatcggtgcc
     2581 ttccagctca atcccaatag aaaaatcatt gcagcgttcg cgcccctgat actgagagac
     2641 tccggcatgc caggcacgtt tatcgaaagg aacatactgg actatttcac catcgcggcg
     2701 aatcaaacag tgagcggaga cgcgcaaatg ggcgatctca gcaaagaaag gatgcgcctg
     2761 cggatcaata gttccagtga ataatgcgtc gatccacgga ccgccaaact cgcctggcgg
     2821 caggctaata ttgtgcacca ccagcagggt gggtgtttcg tcatccgggc ggcaatcgta
     2881 atgtggtgag ggaacgcggc gcgcgccaac cagccacccc tgttctaaca acatgcagga
     2941 tctccttata tgtggtgcta atacccggtt cagagtagca tgtttctacc ttatgattcg
     3001 ttagctatct ggagttttaa catgccgcct cgccgctata accctgacac ccgacgtgac
     3061 gagctgctgg aacgcattaa tctcgatatc cccggcgcgg tggcccaggc gctgcgggaa
     3121 gatttaggcg gaacagtcga tgccaacaat gacattacgg caaaactttt accggaaaac
     3181 tctcgctctc atgccacagt gatcacacgc gagaatggcg tattttgcgg caaacgctgg
     3241 gttgaagagg tgtttattca actggcaggc gacgatgtca ccataatctg gcatgtggat
     3301 gacggcgatg tcatcaacgc caatgaaccc ttgttcgaac ttgaaggccc atcccgcgtg
     3361 ctgttaacgg gcgaacgcac tgcacttaat tttgtgcaaa ccctttcagg ggttgccagt
     3421 aaggtacgcc actatgtcga attactggag ggcaccaaca cgcagttgtt ggatacgcgc
     3481 aaaaccttac ccggcctgcg ttcagctctg aaatacgcgg tactttgcgg cggcggagcg
     3541 aatcaccgcc tggggctttc tgatgccttc ctgatcaaag aaaaccatat tattgcctcc
     3601 ggctcagtgc gccaggcggt cgaaaaagcg tcctggctgc acccagatgc gccagtagaa
     3661 gtcgaagtag agaatctgga agaacttgat gaagccctga aagcaggtgc cgatatcatc
     3721 atgctggata acttcgaaac agaacagatg cgcgaagccg tcaaacgcac caacggcaag
     3781 gcgctactgg aagtgtctgg caacgtcact gacaaaacac tgcgcgaatt tgccgaaacg
     3841 ggcgtggact ttatctccgt cggtgcgcta actaaacacg tgcaggcact cgacctttca
     3901 atgcgttttc gctgattgct gacagattta tccgctccat cgcgggcgga taatctcccc
     3961 tttcccaact ttcttcgtaa cgcctcgcaa atttcttgtc tttcgctgca acgtaataaa
     4021 aaactccgga agctgctttc ccgtttcgtt ttttgcccct cgcttgtgct gatggcgttt
     4081 gccaaagtag cgccaaccaa atcaaggagc gaaacaaatg gacaagcaac gcggttttac
     4141 acttatcgaa ctgatggtgg ttattggcat cattgccatt ttaagcgcca ttggtattcc
     4201 cgcttatcaa aactacctgc gcaaagccgc actcaccgac atgctacaaa cctttgtgcc
     4261 ttaccgtacc gccgtagagt tgtgcgcgct ggaacatggt ggattagata cctgcgacgg
     4321 tggcagcaat ggcattccct cgcctaccac cacccgctat gtttcagcca tgagtgtggc
     4381 aaagggcgtg gtgtcgctga ccgggcaaga aagtctcaat gggctaagcg tcgtcatgac
     4441 accgggttgg gataacgcaa acggcgtcac cggctggacg cgcaactgca atattcaaag
     4501 tgacagcgca ttgcagcaag cctgcgaaga tgtctttcgc tttgatgacg ccaactaagg
     4561 agcggcaatg aatattccac agctcaccgc cctgtgtctg cgttatcagg gagtcttgct
     4621 ggatgccagc gaagaggtgg ttcatgttgc ggtggtcgat gccccttcgc atgagctact
     4681 ggacgcattg catttcgcta ccaccaaacg tattgagatc acctgctgga cgcgccaaca
     4741 aatggaaggt cacgccagtc gcacccaaca gacattgccc gtagctattc atgagaagca
     4801 tcagcccaaa gcagagttgc tgactcgaac gttacaatct gcgctggaac aacgcgcgtc
     4861 tgatattcat atcgaaccag cggacaatgc ctaccgcatc cgcttgcgta tcgacggtgt
     4921 attgcatcct ttaccggatg ttttaccgga tgccggagtc gcattaaccg ccagattaaa
     4981 agtgctgggc aacctggata ttgcggaaca tcgcctgccg caggacgggc aattcactgt
     5041 cgaactggca ggaaacgcca tctcatttcg tattgcgacc ttaccatgtc ggggtggtga
     5101 aaaggtggta ttaaggttgt tacagcaggt gagtcaggca ctggatgtca acacgcttgg
     5161 aatgcagccg ttacaactgg cggactttgc tcatgccttg caacaaccac agggactggt
     5221 gctggtaaca ggccctacag gcagcggcaa aacggtcacg ctttatagtg ccctgcaaac
     5281 gctgaatacc gctgacatta atatttgtag cgtcgaaaat ccggttgaga tccccatagc
     5341 cggactaaac cagacgcaaa tccatccgcg tgccggactc acctttcagg gcgttttacg
     5401 tgcgttattg cgccaggatc ctgacgtcat tatgatcgga gagatccgcg atggcgaaac
     5461 ggcagaaatt gccattaacg ccgcacaaac gggtcatctg gtattgtcca ccctacacac
     5521 caactccacc tgcgaaacgc tggtacgttt acagcaaatg ggagtcgccc gctggatgct
     5581 ctcatcagcg cttacgctgg taatagccca gcgtctggta cgcaaacttt gcccacattg
     5641 tcgccgacag caaggggagc caatccatat tccagacaat gtatggccgt cgccgctgcc
     5701 ccgctggcaa gcacccggtt gtgtacattg ctaccacggt ttttatggtc gcacggcctt
     5761 atttgaagtt ctgcccataa caccggtcat acgtcagctt atttccgcta ataccgacgt
     5821 tgaatcgctg gaaacgcacg cccgacaggc gggtatgcga acgctttttg aaaacggctg
     5881 tctggccgtg gagcaaggct taaccacctt tgaagagtta atccgcgtat tggggatgcc
     5941 gcatggcgag taagcaactc tggcgctggc gtggcataac cggcgacggc aatgcgcaag
     6001 atgggatgct atgggcagag agccgtgctt tgctgcttat ggcactacag caacagatgg
     6061 ttaccccact aagcctgaag cgaatcgcca tcaattctgc gcagtggcga ggagataaaa
     6121 gcgcggaagt cattcatcaa ctggcgacgc tactcaaagc cgggttaacg ctttctgaag
     6181 ggctggcact gctggcggaa cagcatccca gtaagcaatg gcaagcgttg ctgcaatcgc
     6241 tggcgcacga tcttgaacag ggcgttgcct tttccaatgc cttatttccc tggtcagagg
     6301 tatttccgcc gctctatcag gcgatgatcc gcacgggtga actgaccggt aagctggatg
     6361 aatgctgctt tgaactggcg cgtcagcaaa aagcccagcg tcagttgacc gacaaagtga
     6421 aatcagcgtt acgttatccc attatcattt tagcgatggc aatcatggtg gttgtggcaa
     6481 tgctgcattt tgtgctgccg gagtttgctg ctatttataa gaccttcaac accccactac
     6541 cggtactaac gcaggggatc atgacgctgg cagactttag tggcgaatgg ggctggctgc
     6601 tggtgttgtt cggatttctg ctggcgatag ccaataagtt gctgatgcgc cgcccgacct
     6661 ggcttatcgc gaggcagaaa ttgctgttac gcatcccgat tatgggttca ctgatgcggg
     6721 gacaaaaact cacgcagatc tttacgattc tggcgctgac tcaaagtgca ggcattactt
     6781 ttttacaggg cgtagagagc gtcagagaaa caatgcgctg cccgtactgg gtgcaacttc
     6841 tgacacaaat ccagcacgat atcagtaacg gtcaacccat ctggctggcg ctaaaaaatg
     6901 ccggtgagtt tagcccactc tgtttgcaat tagtgagaac agaagaggca tccggctcgc
     6961 tggatctcat gttagacaac ctcgcccatc atcatcggga taacacaatg gcgctggcgg
     7021 ataacctcgc agccttactg gaaccggcgt tgctgatcat aacgggagga attatcggta
     7081 cgctggtggt ggcaatgtat ctgccaattt tccatttagg cgatgcgatg agtggaatgg
     7141 gataacgctg gcgtgttgct ccacgccagc gttgggagat tacaggttgt tgaagatgcg
     7201 gttttcttgt tccagcacac ggataaatgt ggtgcgcttg gtcagctctt tcaggcgtga
     7261 agccccaacg tatgtacaag ctgaacgcag gccgcccaaa atatcacgcg cggtattttc
     7321 aaccgggcct cgcagcggca gcttaacggt tttaccttct gctgcgcgat attccgcaac
     7381 gccaccaacg tgacgtttca tcgcagactc ggagctcatg ccgtagaaca gcataaattt
     7441 ctcgccgttc tcctcaacga tgcgaccgcc gctctcttcg tggcccgcca gcatgccgcc
     7501 aagcatgacg aaatcggcac cgccgccaaa ggctttcgcg acatcgcccg gcgtggtgca
     7561 gccaccgtcg ctgacgatca ttccgcccag accgtgcgca gcatcggcac attcgatcac
     7621 cgcagaaagt tgcggataac cgacgcctgt tttgacgcga gttgtacaaa cagaacctgg
     7681 gccaatgcca actttaacga tatcagcacc ggagaggata agctcctcac acatttcacc
     7741 agtcactacg ttgccagcac aaatggtttt ggtcggccac gcttcacgcg ctttcgcaac
     7801 gaactgcacg aagtgttcgg aataaccatt cgccacgtca atacaaacga agtttaatgc
     7861 cgggttcagg tcgagaatct gtttagtttt ttcgaaatcc gcatcagacg tgccggtaga
     7921 aaccatcaca tgtttcagca catcagcgga agaattgttg ataaacgctt gccactcttc
     7981 gaaagaatag tgtttatgca cagcagtcaa aatatcaaaa gaagccagcg cagaggccat
     8041 agaaaatgtg cctacggtgt ccatatttgc ggcgataatc ggcacgccgg accagctctg
     8101 acctgaatgt ttgaaggtga attgacgttc cagttcaaca tcggaacggc ttttaagagt
     8161 ggagcgttta gggcggatga gaacgtcttt aaaacctaac ttcagatctt cttcaatacg
     8221 catgtgcgga ttcctggggt taatggctat aagggtaaga gcgcaactcc agtgacgcta
     8281 tcatacgcac taatcaatgc ggcgcaagac tgcgaaattg tcttttttta cgctacaatc
     8341 ccataaattt acctggtaaa tagtgagcga acgtttcatc aatttcgctt tcgtgcgctc
     8401 atttttaacg ttttgataat catgatgaat tcccgggaat ataaattatg aggtatatag
     8461 ttgccttaac gggaggcatt ggcagtggca agagtaccgt tgccaatgcg tttgctgatc
     8521 tcggaattaa cgtcattgat gccgatatta ttgcgcgtca ggtggttgaa ccaggtgcac
     8581 ctgcgctaca tgccattgct gatcactttg gcgctaacat gattgctgct gatggaacat
     8641 tgcagcgccg ggccttgcgc gagcggatct tcgccaaccc ggaagagaaa aactggctta
     8701 acgccctgct gcatccgctg attcagcaag agacgcaaca ccagatccag caagcaacct
     8761 ccccctatgt actgtgggtt gtgccattgc tggtagaaaa ctcactgtat aaaaaagcga
     8821 atcgagtgct ggtggtggat gtcagcccag aaacgcaact taagcgcacc atgcagcgcg
     8881 atgatgtaac tcgcgagcat gtcgaacaaa tccttgctgc tcaggcaccg cgcgaagctc
     8941 gccttgccgt ggcagatgac gtcattgata ataacggcgc accggatgct atcgcatcgg
     9001 atgttgcccg cctgcacgca cactatttgc agcttgcgtc gcagtttgtc tcacaggaaa
     9061 aaccgtaatg cagacccagg tcctttttga acatccacta aatgaaaaaa tgcgtacatg
     9121 gctgcgcatt gagtttttaa ttcaacaact caccgtaaat ttgcccgtcg ttgactacgc
     9181 tggcgctctg catttcttcc gcaatgtcag tgaattgctg gatgttttcg agcgcggcga
     9241 agtccgcact gagttgttga aagaacttga ccggcagcaa cgtaaactcc agacctggat
     9301 tggcgtgcct ggcgtggacc agagccgtat tgaagcatta attcagcagt taaaagcggc
     9361 ggggagcgta ttaatttccg cgccccgtat cgggcaattt ctgcgtgaag atcgtttgat
     9421 tgccctggtg cgtcagcgac tgagcatccc cggcggctgt tgtagctttg atttacctac
     9481 attgcacatt tggctgcatc taccccaggc gcagcgcgac agccaggtag aaacctggat
     9541 tgccagcctg aacccgctca cccaggcact taccatggtg ctggatttaa ttcgccagtc
     9601 ggcccccttc cgtaaacaaa ccagcctgaa tggtttttat caggataacg gtggcgatgc
     9661 cgacttgctg cgcctgaatc tgtcactcga ttcacagctt tatccgcaaa tttccggtca
     9721 taagagccgt tttgccattc gttttatgcc gctggacagt gaaaacggac aggtaccgga
     9781 acgtctggat ttcgaactgg cctgttgcta aggagtaaaa atgtcagaaa ctattacggt
     9841 gaattgccca acctgcggga aaacggtggt gtggggtgaa atcagcccgt ttcggccatt
     9901 ttgctccaaa cgttgtcagc tgatcgacct cggagaatgg gctgctgaag aaaaacgaat
     9961 ccccagcagt ggcgatcttt ccgaaagcga tgactggagc gaagagccaa agcagtgaca
    10021 ttttatctga tgcgacgctg gcctgataag acgcgggcag cgtcgcatca ggcatcgtgt
    10081 gcaaatgtcg gatgcggcgg gaacgcctta tccgacctac aagaccttac atatcaacat
    10141 gttaaaattg tagatactga taagacgcgc cagcgtcgca tcgggcatct tgtgcaaatg
    10201 ttggatgcgg cataaacgcc tatccgacat acacacgctt aagcttcgca agtaccggtt
    10261 cattggctgg cggaaaatca tcggcattca gaccgaccag cgacatccac tcaccgggtt
    10321 gcccttcttt accccacggc tccccttccc agctttcgac cagccagaac cacagtgtta
    10381 tatgcctgtc cgggaattca tattccagta tttcaaatag cgaaagatgt tgtggggtaa
    10441 tcccgacttc ttcctgaagt tcacgcacca ccgcctgttc cggcgtttca cccatttcaa
    10501 ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgggtta
    10561 taaagatttc attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg
    10621 tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta
    10681 ttgcaggcgg ccatggcact gcttatattt tttaccagaa ccacacgggc aaggatcgtt
    10741 acgtcctact ttgcgttcac cggtttgcgc cgccagtgca gctgcggcag cagagtcgtc
    10801 atcctgatgg ctaagctgct gcatttgcgc taaacgctcg gcttccatac gacgctgttg
    10861 ttccagctcc tcaacctctt caggcatacg tacctgaact ttgctcagcg tgctgataac
    10921 ttcatatttt aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc
    10981 ctgcttcgga tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat
    11041 cgcagccagg tgctctttcc acagagagtc aagcgtttgc agcatgacgc ctttctcgaa
    11101 gtgacgcatc atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg
    11161 cgccagaatg cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc
    11221 ggcaattggc aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc
    11281 ccacatttct tccagcgact gtggcggaat gtaggcatca atggtcgctt tgaacacatc
    11341 ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg
    11401 ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg
    11461 aatgtcgaag ttacggcttt caactttacg ctgggcgttg gcaatcgctt tggtcaccca
    11521 cgggtgctca atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg
    11581 gtcggaagca aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc
    11641 agcatccccc tgacgaccag aacgaccgcg cagctggtta tcgatacgac gggattcgtg
    11701 acgttcagta ccgatgatat gcaggccacc tgctgccagt accgcatcgt gacgtacctg
    11761 ccagtcggct ttaatttttt caatttgctc tgcagtcgga ttttccagcg cggcaacttc
    11821 tgcctgccag ctaccaccga gcacaatatc ggtaccacga cccgccatgt tggtcgcgat
    11881 agtcaccgca gccgggtaac ctgcctgagc aacaatcgcc gcttcgttgg catggaattt
    11941 ggcgttcagg acgttgtgct taataccggc tttggtcagt tcatttgaca ccagctccga
    12001 tttttcgatg gagattgtac ccaccagcac cggctggcct ttcgcagtgc gttctttgat
    12061 atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc
    12121 tttacgaatc attggacggt tggtcggaac aacgacagta tccagcttat agatggagct
    12181 gaattcgaaa gcttcggtat cagcagtacc agtcatcccc gccagttttt catacagacg
    12241 gaagtagttc tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc
    12301 ttctttcgct tccacagcct ggtgcagacc atcggaccag cgacggccct gcatggtacg
    12361 accggtgtgt tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcgcg
    12421 ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc
    12481 cggagagtac agagactcac cttcatccat gatgccttct ttaaccagca gttcttcaat
    12541 cagaaccaga ccacgttcgg tcaggttcac ctggcgagat ttttcatcca ccgagaagtg
    12601 gccttcgccc tggaaggttt cggagtcttc tttttcctga cggatcaggt gtggaataat
    12661 tttattcacg cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg
    12721 tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt
    12781 acgctgtaca cgttcttcag gactgaacgc catgttgtcg cgcaggtagt caaagccgta
    12841 ttcgttgttc gtaccgtaag taatgtcagc tgcgtaagct tcacgctttg ccggtgctgg
    12901 catgcccggc aggttgatac caactgtcag gccaagaaat tcaaacagcg gacggttgtt
    12961 ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt
    13021 tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat
    13081 ttcggcgatg cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg
    13141 cataccaaag acgcgcttac ttgcctcacg caccacggcg aaagcttccg ggatcagatt
    13201 ttccagcact tcgccttttt ccagacgtgc acgaaactct gcggttttcc ctttcagttc
    13261 ttcgtcggag agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg
    13321 catccggcgc agggtgcgat cgttacgact accgaaaact ttagttaata atttgattag
    13381 cataataaaa tcccaaacgc cccgcgttgc ggagttaata aaatgaagta acggtttatt
    13441 gttgttagct gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg
    13501 gcgtgctgaa ttttgcttgc ggggtaaaat gcgcataatc aatgcgataa cccttttcag
    13561 acggcgtgcc ttcctgggtc aacagcgcgc tgagcgtatc cagtaatgca agatgttgcg
    13621 cctgaagagg caaagattct tcagcaacgg gcagcgtttg cggtgccatt gcgaaggaaa
    13681 gatgacggat taccgtgcga atggcatgtt gatgccagta atcaacggaa tagttcgaat
    13741 tcgggcgacg cgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag
    13801 gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg
    13861 caggcaaacc taaactcgcc gcgaccatcc ctaataagag atgcggccag aagtagcgtt
    13921 taccaaactg tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgtatt
    13981 caaacgtatt tagcgcacaa atcttatcat taaagcctga agtccacagc acgaatgaag
    14041 ataagttgtg cgtaaaagtg cttaagaaaa cagcgttcgc accgtttttt atcgttctta
    14101 taaaattcaa aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc
    14161 gaattttata cgacagtata aatgtcgtta tgccagtacg gctgaaggcg ctttgaaggc
    14221 caacggcagt tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag
    14281 gacagcctgc agcagtttgt tattcagtgc atgaccagat ttataagcgg taaatgcacc
    14341 aataatattg tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac
    14401 aaattcgtct tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat
    14461 ggcacaatcg aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg
    14521 catgaaaccg aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt
    14581 catcgcatag cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc
    14641 cagcgaaaaa ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac
    14701 agtctctttg atgcgaacaa atttcttggc gcagttcagc tcgtcgatac cagcgtcaag
    14761 cagcaggtat acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac
    14821 ttcgataaca atgttatcga tgcccaggcc cgcgagagca gcattgaggt gctctacggt
    14881 tgaaatccgt acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga
    14941 tttggcatcg gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc
    15001 ggtgttggcc ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac
    15061 acccgtcgcc tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca
    15121 aattacctat ccaaccgaag tgtactatac attcggcggg ccagtttagc acaaagagcc
    15181 tcgaaaccca aattccagtc aattcttaat cagcttgctt acgcaggaat gctgggatat
    15241 ccagataatc cggctctttc gcagtttgcg gcgcattgtc attcacgact ttagcaaccg
    15301 gcttctgctc ctgggtcagc ggagccatcc cgtgctgctg gtagcgatcc atcactggct
    15361 gctgaacctg cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg
    15421 tcgcaacaac agttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa
    15481 ccacagtcgc gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat
    15541 ccagacgcag gtcgaagccc gctgtgatgt ttaccagtac gccgcgtgcg ccagacaggt
    15601 cgatatcttc cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacgatctt
    15661 caccgctcgc cacaccagaa cccatcattg cgtagcccat ttcagacatt acggtgcgta
    15721 cgtctgcaaa gtccacgttc atcaaacccg gacgagtaat cagttcggcg ataccttgca
    15781 cagcgccttt gagtacgtcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc
    15841 ccagaacttt cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca
    15901 gttcagtgat cccctgctcc gcgaatgcca tacgcttctt gccttcaaag ttgaagggct
    15961 tagtgacgac agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggag
    16021 ctgcgcctgt accggtacca ccacccatac ccgcagcaat aaagaccatg tctgcacctt
    16081 ccagcgccgc acgcagtgca tcgcgatcct catcagccgc attgcggcca acttctggat
    16141 tagcgccagc tcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg
    16201 ctgttttacg cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatgc
    16261 gctcgcgcac catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa
    16321 tcaccgcgtc attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg
    16381 tcgcctgaga ccgtaatcat tgtcggcctc ataaaaatta aaactctttt cgcagccaac
    16441 tattgagtcg cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt
    16501 caccgttaag atgtgactct ttcccgtaat gaagcaatcc caccgccgtc gaataatacg
    16561 gctcctgagc ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacctgcg
    16621 tatgaaacac gcgctgagca caggctgcaa gaccttcaat ctgcgctgcg ccaccggtta
    16681 atacaatgcc tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact
    16741 gcaatatctc ttcgttgacc aggttgagca gctcggtata gcgcggctcg atcacctctg
    16801 ccagggtctg acgttgcaga ctccgcggcg gacgaccgcc tacgctcggc acttccacgc
    16861 tctcatcttt tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg
    16921 cgtcgcttgg cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat
    16981 aaggaattac cttagtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac
    17041 caccaccgat atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat
    17101 aacttgatgc cagtccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg
    17161 ctttgacgat gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca
    17221 tccgcacgcc cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact
    17281 cttgcgggat cacatgcagc acacggtgtt catcgcgcac acgcaccgat ttcgcggtat
    17341 ggacaacgtt ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa
    17401 tttcattctg gcagctgatg tgcttaccag aaagcgccag atataccgaa gagatctgac
    17461 aatctgccat caattctgcc tggtcaatgg cgcgctgtac gcacttgacc acggattcga
    17521 ggtcgttcac cccgccttta tccataccac gcgacgggca gctgcccacg ccaatgatat
    17581 tgaccatacc gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaatct
    17641 ccagtcctac taccagtttt ctgtccgtcg ccttaatcat tgttgttctg cctgtgcctg
    17701 attttgttgc tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga
    17761 gtcataacgc aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa
    17821 aaccggataa agttctacaa agcgagccaa acgtttcatc gtatcgcccc ggccaagatt
    17881 gagcttaata tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc
    17941 cttcagagta aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac
    18001 ttcattggcg ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc
    18061 tggcggcacg ctgaaagtgt ttccttccgc gtctaccata tgttgatcat tccaccgcgc
    18121 aatcggcaca tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct
    18181 cacctgctta atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcctg
    18241 ggtcataaag gtacctggct cacccaatgc caggatcgac tgccggatat cgtcattacg
    18301 cgtgtaatgg cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc
    18361 catccagccc aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa
    18421 aaggatcccc gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctgtt
    18481 tcgcgtgttc agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca
    18541 actgcgagaa gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca
    18601 tacccggtga ggtattggct tccagcagat aaaactgtcc atcgctgtcc agcataacgt
    18661 caatacgtcc ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca
    18721 aattggcctc ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga
    18781 gatacttcgc ctcataatca tagaaggttc cggacggttg aatacgtatt gacggtaaaa
    18841 tttcttcacc gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata
    18901 cttcttcatc gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta
    18961 cttttgacat tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca
    19021 gagcagaaat ttctgctaac tgcttatcac tcaggccttt ttcaaactct gcgcgggtta
    19081 acgctaccca cggagcgacc ggtaaaccgg caccttgcca cagaagtttg ctgcgtagtt
    19141 tatccattga aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct
    19201 cgagcatgcc ctgcagcgtg ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt
    19261 tctgaaagcc catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggattcg
    19321 cgtcaatacc gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt
    19381 cccgctcagc ggaggtccca cccaacagga ccgcgatttt atcagtcatg ttgttcttcc
    19441 tccggagttt gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta
    19501 ccagccccct gaacgagaat caggtcgtta ccggttaata ccggagccag catctcggct
    19561 acctgcgccg ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga
    19621 cacagcgaac ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc
    19681 atcaacaggg tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta
    19741 cgggtaaaac ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcacgc
    19801 gccgctttaa tggtggcgtc cacttccgtc gggtggtggc cgtagtcatc gaccagcatt
    19861 gccgtaccgc ttttaccatt cacaggctcc agcgggaatt caccgaggaa atcaaaacgg
    19921 cgaccagtcc cctggaagct ctccagcgca cgcaaaatag cctcgtcgtc aattccctct
    19981 tccgtagcaa ccgcaaccgc agctgcggcg ttcagcgcgt tatgacggcc tggcgcattc
    20041 agggtgacgc gcatcggctc tttgtcctgg cgcagcagcg taaagtgccc ctgcgggcca
    20101 atctgctgat aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agtcgtggtc
    20161 tgacgtccca cacgcggtaa taattcgcgg atcaccggat catcaacaca catcaccgca
    20221 cgaccgtaaa acggcaggtt gtgcagaaaa ttaataaagg tctgttttaa attctcaaag
    20281 tcgccctggt aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt
    20341 tgcagatgca ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc
    20401 cccaaacgcg catgaacgcc cgccgctttt accagcccac cgttaacgaa ggttgggtcg
    20461 agtcccgctt ctgcgtagat gctggaaacc atcgcagtgg ttgtcgtttt gccgtgcgtt
    20521 ccggcaatgg cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg
    20581 atcaccggaa tacgcgcttc atgagctgcg acaatttccg ggttatcggc agaaatcgcg
    20641 ctggaaacaa cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc
    20701 gtcgccccaa gattcattaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg
    20761 atctgataac cttcattggc cagaacttcg gcaataccgc ccataccggc accaccgatg
    20821 ccgacaaaat gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc
    20881 aattgttgtg tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc
    20941 atcgctacaa ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat
    21001 ccggaatgga tgcggcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc
    21061 cggccagggt gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg
    21121 cgcccgcttt ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg
    21181 gcacaaacaa cgccggtagt cctgccgcgg cgatttcact caccgttaac gcaccggagc
    21241 ggcaaacgac gacatccgcc cacgcatacg ccgccgccat atcatcaata aattccgtca
    21301 ctttatgctg cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt
    21361 taccgctctg atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg
    21421 tctggttaag aatgcgtgcg ccctgggaac cacccactac caacacacga accggacctt
    21481 cacgtccagc caaacgttgc tgcggcaacg gcagcgccag cacatcggta cgcaccgggt
    21541 tacccaccac ttccgcatta gggaaagcgc ctggaaacgc ctgcatcact ttggtggcaa
    21601 tcttcgccag ccatttattg gttaatcccg caataccgtt ttgttcatga agcacaaccg
    21661 gaatacctaa cgaccacgcg gccagaccac ctggacctga tacgtagccg cccataccga
    21721 gcaccacgtc aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga
    21781 tacgcagcgg ggcagctatc agtgctttta taccttttcc acgcagacca gagatacgaa
    21841 tgaaatcaat ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc
    21901 ccagccagcg aacttgccaa ccctgagcca ttagatggtg cgcaaccgcc agtcccggga
    21961 atacatgtcc accggttccg cctgccatca ccattaatcg cttcccttga ccactcatcg
    22021 tgaacctcgt acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag
    22081 catcatgatg gctgtcgaca taatcagtaa gctcgaacca ccgtaactga tcagcggcaa
    22141 tgtcagacct ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctggaa
    22201 actaaaccag ataccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa
    22261 tgctttgcgg ccaatcgaca tcgcgcgaaa agcgacgaag aataccatta aaagtgccag
    22321 caccacaccg acatacccca gttcttcgcc gataatggcg aaaataaagt cagtgtgcgc
    22381 ttccggcaga tactccagtt tttgtaccga gttacctaaa ccttgtcccc acagttcacc
    22441 gcgaccaaac gccatcaacg attgcgttaa ctgatagccg ctgccaaagg gatcttccca
    22501 cggattccag aatgcggtaa cacggcggat acggtacggt tcggcgagga tcagcaacac
    22561 aaccgctgaa atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag
    22621 gaacaacatc gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc
    22681 cagcagtaac actgccaaca ccagaattac gcccatcggt ttcaggaagc cgcgcaggtt
    22741 attacgtact tcgtcgcctt tacgcaccag atagttggcg atatagcaaa acagcgacag
    22801 ttttgtcagc tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc
    22861 tttaaccgag ctacccacta ccagaacgat catcagcagg atgatagatc cgagcagcat
    22921 cgtggcactg tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat
    22981 gaatgccagg atcagataga cgccatcacg cttcgcgaag aagaacggat cgttggttaa
    23041 gcgttgccct atgggcattg acgccgaggt caccatgata aagccaatcg ccgcgaggcc
    23101 gaaggtcagc cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg
    23161 cgagcccatc acccagccct ttagcgccgt ggagatccag accagaatac tgaatcctgg
    23221 caggcgcggc attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga
    23281 cgggcaaact cattgcctcg ttgttcaaag ttcttgaatt gatcaaggct ggcacaggcc
    23341 ggggagagca gaaccatatc gcccggctga acacgcggag ccagcaagcg catcgcctgt
    23401 tccatggttt cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcgccgtcc
    23461 cgaccgaaac aatacagacg tacattatcg ccattcaggt aacgcgccag tgggctaaag
    23521 tccgccgatt taccatcgcc acccagcaac aaatgcagcg tgccgtctac atgcaggcca
    23581 ttcagcgccg cttccgtact gccgacgttg gtcgcttttg aatcgttaac ccagcgcaca
    23641 ccgttatgct ccagcacaac ttcaaagcga tgcggcagac cagtgaatgt ggttaacgct
    23701 ttcaggctgc tggcacgcgg taaccctgcg gcatctgcca gcgccagcgc cgccagcgca
    23761 ttggtgtagt tatgctgccc ggaaagtttc atttctttca cgttcagcac tttctcgcct
    23821 ttcacccgca gccaggtttc accctgctga tgattcaggt gatagtcgcc catgttgacg
    23881 ccaaagctga cgcagcgttc atccgcaccg cgaatcggca ttgttaaggc atcatcagca
    23941 ttaaccacgc aaatttttgc gttttcgtaa atgcgcagtt ttgctgcacg atactgttgt
    24001 aaaccaaacg gatagcgatc catatgatct tcagtcacgt tcagaatggt cgctgctacc
    24061 gcctgtaaac tggaggtggt ttcaagctgg aagctcgaca gctccagcac gtacagttcg
    24121 cactcatcat ccagcagcat caacgcaggc aggccaatat tgccacccac accaacgtta
    24181 acccccgccg ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa
    24241 ccggtgaccg ccacaatcgg tgcttgtgct tcacgacaga acagctcgat atcgccaacg
    24301 atttcgattc cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga
    24361 ctggcgacaa tcagatcggc cgccatcagc cattcatcat tcagaccgcc cgtgtggcgt
    24421 tctacagctt cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc
    24481 ggcgtcacac cgcgagcgag gaaaaagtcc acgcaagaaa gcccggtgag gcccaggccg
    24541 ataatgacga catttttacc ctggtaatca gccatgatta acgtaccttc agcgttgcca
    24601 gaccaatcag gaccagcatc agcgaaataa tccagaaacg tacaatgacg cgcggttccg
    24661 gccagccttt cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc
    24721 gcagtttaaa ggagccgacc tgcaggatga cggaaagtgt ttctaccacg aacacgcccc
    24781 ccataatcac cagcaggaat tcctgacgca gcagtacggc gataatacct aacgcaccac
    24841 ctaacgccag cgaaccgaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca
    24901 ggaagcccag ccctgccccg actatcgcgg tacagacaat gaccagttcc ccggcgtgtc
    24961 gtagatacgg tatgtgtaaa tagctggcaa agttcatgtt gccggtcgcc cacgccacca
    25021 gcgcaaaacc accggcgaca aatacggtcg gcataattgc cagaccgtcg agaccatcag
    25081 tcaggtttac cgcgttgcct gtacccacaa tgacgaagta agccagcaga atgtagaaca
    25141 gccccagttg cggcatcaca tctttaaaaa atgggaccac cagctgcgtt gcgggcgtgt
    25201 ctttgccaac aaggtacagc gcgaaggcga cacccagcgc aatgaccgac atccagaaat
    25261 acttccaacg agcgatcaac cctttggtgt ctttacgcac tactttgcga taatcatcaa
    25321 caaagccaat aacaccgtaa cctaccagca ctaccaacac gcaccagacg tacggattgg
    25381 acgggtaagc ccacagcagt acggagatca caatcgctgt caggatcata atcccgccca
    25441 tggtcggcgt accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc accacctgac
    25501 caaaggaaag tttttgcaaa tgagcaatca tgcgcgggcc catccacaat gagatgaaca
    25561 gcgcagtcag caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg
    25621 aataatattt gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc
    25681 gcgtactacc tcttccatgg cagcactacg tgaaccctta actaaaatcg taattacctg
    25741 ttgctcagca atcagtgatt taagacgcgc gataagcgca gttttatcgg aaaaatgttc
    25801 gccaacaccg ctggcagtgc tgatagcatg gctttgtttg cccacgctta acacgcggtc
    25861 aataccagct gcttttgccg cttcgcccac ctgtatatgg caggcttcgc tttcagcgcc
    25921 cagttccgcc atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagtacctg
    25981 gactgctgcg gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt
    26041 ttccgccagt tggatgggga acagacggcc tggaacagct ttcagatttg ccagccccgc
    26101 tttgatagca tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt
    26161 cgcaatattg tgacgccccg gcaacggcag cagaacatcc acgctaccgg ttggggtttg
    26221 cagggtaaat tccgtaccgt gcgaagtcac atggatgttg gtggcggtga aatcgctgtt
    26281 ggcggcatta ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag
    26341 ccagtcgttg ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat
    26401 ttcacctttc gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc
    26461 caggttgttg accagcgccg cttccgggcg agtcagactc acagtccagg caatttcgcc
    26521 ctgatggttc gcgccaagtt caattactgc gtaatcgtac tctggcgtta agcgcaacag
    26581 cgtcatcggt acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccaca
    26641 ctggcttaaa atcgctgccg tcatctcttt aacggaggtt ttgccggagg aaccggtcag
    26701 agcaaccacg cgcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag
    26761 acgagtatcc ttgacgatta cctgcggcag atcgatatcc agcggacggt taaccagtag
    26821 tgcacccgca ccgccagctt tcgcctggtc agcaaaatca tgggcatcga aacgttcgcc
    26881 tttcagggca acaaacaggc aacccggcgt cagttttcgt gtgtctgtgg ttacagcatc
    26941 aagggtgata tctgcccctt ttagttcacc gttgagaata tcggtaagtt ggctaagggt
    27001 tacgctaatc atgcaatcac ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc
    27061 agacgctgat tgccaacaat ctggtaatcc tcatgacctt tgcccgcgac cagtaccaca
    27121 tcattctctt tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact
    27181 ttggcatgtc cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct
    27241 tcggtacgcg ggttatcgtc cgtcaccacc gccacgtcag cgaactcttc agcaattgcg
    27301 cccatcagtg gacgtttgcc tttatcacga tcgccaccac agccaaagac gcaccacagc
    27361 ttgcccgcac agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggtgtatgc
    27421 gcgtaatcca ccactaccgt cggtttgcct ggcgcagtga acacttccat acgtccacaa
    27481 accggctgca ggcgtgcggc agttttcagc aggtcagcca acggatagcc cagcgccagc
    27541 agcgtcgcca gcgccagcag caagttgctg acgttaaaag cgcccatcag atggctttca
    27601 atttcgccat cgccccagct tgagctaaag cgaatcgtcg caccgctgtc gtgatagttc
    27661 acttctgtcg ctttcaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat
    27721 accgcaaccg cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta
    27781 ataatcgcct gaccgcaatg atgctcagaa taaagcagcc atttcgcggc ttcgtagtgt
    27841 tccatatcac catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgca
    27901 aatttcaatg ccgccacacg gtgctgtacc agtccgtggg aggaaacttc cattgcgcaa
    27961 aaagtcgcgc cctgatccac cagccccgcc agctcatgct gaacatcgac tgccgaaccg
    28021 gttgtatttt ctgtcgggat cactttcccc agcaggccgt taccaacggt gcccattacc
    28081 gcgctggttt cgccaagcag ttggctccac tgcgccagca gctgggtcgt cgtggttttg
    28141 ccgttggtgc ccgttacgcc cacgagacgt aaattgtcag agggttcatg gtaaaagcgg
    28201 cccgccagtg cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc
    28261 atttcacgga tttcaccgtc ggtcgcctca tctttcgcct ctgcaataat ggcagccaca
    28321 ccttgcgcta tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct
    28381 acaaagagat cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc
    28441 gaaggtgcgt ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt
    28501 cccctcgcct tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggttc
    28561 gatgttcatg gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc
    28621 gccgccgtag tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg
    28681 ctgactcgca ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc
    28741 cggcccgacc tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc
    28801 cttcacgccg ccgccgcccg gtagcgccac gctttccatc atatgcacca cggtgcgaac
    28861 aatggattcc gggaagactc gttcaccggg aaccgggggg tcaactttgg taatcgacag
    28921 tgggcgataa atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt
    28981 taccattagc ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt
    29041 ttgaggatat aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc
    29101 aaaacgtgag taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac
    29161 accgacgtta ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac
    29221 gtctttgatt tcgtggccgt taattcgata aggaacggta ttcagtaccg agttttcccg
    29281 cactacgcca cgttgcaacg cggtcatcac caccatcggt ttaaccgttg aacccggttc
    29341 aaacacgtca gtgatggtac ggttacgcat cgcctctttc ggcgtgccac tcagattgtt
    29401 tgggttgtat gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag
    29461 cacggcgcta ccagattcag ctttgttaaa ggccaccgcg ttgttcagtt cgcgataaac
    29521 cagcgcctgc aggcgttcat caatactcag cgccaggttg tgcgccgcct gactgtcggt
    29581 ggaagagata tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg
    29641 ctgcccggta agccatttat cgaaactctt ctcgacgccc tcaatcccct gactatcgac
    29701 gttagtaaag ccgatgaggt gagcggtcac ttcgccggat ggataataac ggcgagattc
    29761 ttcacgcaga tgaatccccg gcagtttcag ttttttgatg tactctgcca tgtcagggtt
    29821 tacctgacgc gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag
    29881 ctgatccagc ggaatattga gtgcgttagc cagcgccttc cagcgatcac cgacgctgat
    29941 accgccagcg tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac
    30001 cgctaacggg cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac
    30061 gcgaagagaa cgcatgtcgc cctcttttac cagcatatcc ggggagataa cttgtaacca
    30121 cgctacgcgt ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa
    30181 acgccaactg ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc
    30241 tgctttcatg cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc
    30301 aacatgctgc atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag
    30361 cgcattctct tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac
    30421 cagttgttcg cgctgtgcgg tcagtaaacg ggtatggtgc gccgtggtta ccacggtcac
    30481 cgccgtcaaa ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc
    30541 gtcaccgata acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt
    30601 gcttagagct tctgtcactc tgctgatcat gcgttcgtcc tctctgcaat acgcagaact
    30661 gaactacggg cacgagggtt ctcagccacc tcttcttcac ccggcattaa cttgcctagt
    30721 gctcgcagct gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc
    30781 ggaacttgcg gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc
    30841 gagtggaagc tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt
    30901 agcgcctgct ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta
    30961 cgggtcgcgg gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc
    31021 agttctttgg tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg
    31081 cgtttggcaa aacgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct
    31141 gcggtttgta gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc
    31201 ggaccatcgc gcataaagga aaagccgcgt tcagcatcat caagttgcgg tgaagagacg
    31261 ccaagatcga ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg
    31321 cccagcgcgg agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca
    31381 acggcgatag cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc
    31441 gagaggatca gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca
    31501 ggacggatat tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt
    31561 tccatcattt tatagagaca agtcctgcag tcgctccgat aagtctccag tagccaactg
    31621 ctctgcgtcg atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa
    31681 cttgttgaac tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac
    31741 tggcgcgatt aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag
    31801 taggcgctgc acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc
    31861 gataatttcc cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt
    31921 gcaaaccatt tgaccggcag cgttctcaag cagctgttcc cgataacggg taggcactga
    31981 taagcgccct ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc
    32041 ccttattccc actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa
    32101 gcttgtcaag ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat
    32161 cagcgttgtt tattttacgt ccaacgtgcg aagcaaatta acgttatgaa tagctgtaag
    32221 aaaaaatccc tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat
    32281 aaagtgtcag tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc
    32341 ctgttgcgtc tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgtt
    32401 tgttaaatcg gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctatcgata
    32461 tcacttaatt attgtgaatt tcagctggtg aggtaattaa cgggcaggtt taaaagcatt
    32521 tatcaataat agccaggaat agtcttattt actttaagga tattgatgtc cattcccgta
    32581 ctcaacgcgc cagagagaaa ttcacctggc gcgtattttt gttcgcggct tagctacggc
    32641 tgagcacgcc gcggcgatag agattacgtt taatgcgcgt taaaccaggt tttggcttac
    32701 gcggttcgtc caggcttgcc aggacaatct ccagcacacg ctctgcgaca tcgcggtgac
    32761 gttgagccac tgccagcacc gggcactgta agaagtcgag cagttcgtta tcgccaaagg
    32821 tggcaattgc caggtcagaa ggcagtttgc cgtcacgacg tagcgtgaca tccatcaccc
    32881 cttgcaacaa cgcaaacgac gttgtgaaca gtgcctgcgg catcggatgc gtttccagcc
    32941 atttttcgaa taactgggca gccgcctccc gctcatagct gttggcataa aggaaatgca
    33001 cttcgcgagg atcatctttc caggcagtac ggaaaccttg ttcacgcagg aagctgacag
    33061 aaagctccgg cagcgcgcca agataaagta ccgtctcggc gggaaactta cgtaactctt
    33121 ccgccagcat ttcggcatca tcctgatcgg caccaaccac gctggtgaag tgttcacgat
    33181 cgagggcgcg gtccagcgcg acaatcggga acgggtcgtt agcccagcgt tgataaaaag
    33241 gatgctcagg tggcaacgac gtcgaaacaa taatggcatc aacctgacgc tgtaaaagat
    33301 gctcgataca acgcatttcg ttgtcgggct gatcttccga gcaggcaatc agcagttgat
    33361 aacccctttg ccgcgcctgg cgttcaagat agttagcgat gcgggtatag ctggtgttct
    33421 ccagatcggg gatcacaaga ccaatagaac gtgtgcgtcc agcacgaagc ccagctgcca
    33481 cggcgttcgg gtggtaattg tgctcacgca ccacagccat gactttttca acggttttgt
    33541 cgctcacacg gtattgcttc gctttgccgt taataacata gcttgcagtg gtccgcgaca
    33601 ctcccgccag ccgagcgatt tcatccagtt tcacaattgc cccttgcgta aaatgtaaaa
    33661 accatagcca ttgtactggt atgggttaaa ttcattaaca tctaagcgca gaaaattaac
    33721 tgcggcaact gcttttattc caggttccca ctgatttcgc aaaaaaagcc caacgtgaca
    33781 tgttgggctg caaattgcgc attgagatca ttctcaacgc attattttat cgccgcgcga
    33841 aagtccgact acaccagagc gagcaacttc cacaattttc gccacatcgc gaatcgatgc
    33901 taaaaatgca tcaagcttat cgctggtgcc tgctaattga acggtataaa gcgagggtgt
    33961 gacatcgata atttgcccac ggaatatttc cgtattacgt ttcacttcat cacgcccgta
    34021 accgctggcc tgaattttca ccagcatgat ttcccgctca acgtgcgcgc cctgccccaa
    34081 ctcgctcacg cgcaagacat cgaccagctt gtgtaattgc ttttcgattt gctcaagcac
    34141 tttttcatcg cccacggtct ggatggtcat acgcgataat gttggatcgt cggttggcgc
    34201 aacggtcagg ctttcaatgt tgtagccacg ctgggaaaaa aggccaatca cgcgggataa
    34261 cgcgcctgat tcattttcga gtaagactga taatatccgg cgcataatca ggttctctcc
    34321 gttttgctta accacatttc atccattccg cccccgcgaa tctgcatcgg gtagacgtgc
    34381 tcactgccat cgacggtgac atcaacaaac accaggcgat tattgcgcac ctgttccagc
    34441 gcctcgctaa gtttgctttc cagttcttgc ggatgagaga tctggatccc gacgtgacca
    34501 taggcttccg ccagacgaac gaaatcgggt agtgactgca tataagattg tgaatgacgg
    34561 ccggaataga tcatgtcctg ccactgtttc accatcccca gatagcggtt attgagattc
    34621 accaccagta ccggcaactc gtattgcaac gctgtagaca gttcctggat gttcatctga
    34681 atactgccgt cgccagtgac gcaaaccacg gtttcttctg gcaacgccat tttgacgccc
    34741 agtgccgcag gtaaaccaaa acccatcgtg ccgaggccgc cagagttaat ccagcgacgc
    34801 ggtttgtcga atggataata aagcgcagca aacatctggt gctgcccgac atcggacgtc
    34861 acgtaagcgt ccccattagt caaccgccaa agagtctcga tcaccgcctg cggtttaatc
    34921 ttttcactgt gagtgtcata tttcaggcac tgacgagcgc gccactgttc aatctgctgc
    34981 caccagtcgc ggatctcatc cagcggttga tggacggatt cttgcgacag gagttcaagc
    35041 atttgttcga ggacctggcg agcatccccc acaatcggga tatcagcagt cacggtttta
    35101 gaaatggaag taggatcaat atcgatatgc agaaccgtgg catttgggca gtacttcgcc
    35161 agattgttcg tcgttcggtc atcaaaacgt accccgacgg caaaaatcac atccgcgtta
    35221 tgcatcgtca tattggcttc gtaggtaccg tgcattccca acatgcccag tgcctgacga
    35281 tgcgttgccg gaaacgcccc cagccccatt aatgaggaaa caacgggcag attcaacgcc
    35341 tccaccgttt cttttagctg ctgatgacag cccgccgtga ttgccccacc gcctacatag
    35401 acaaccggtt tttttgccgc caccagcgtt tgcagagcgc gcttaatttg ccctttatgc
    35461 ccggaggtcg tgggattgta agaacgcata ctgaccgact ccggccagac atagggtaat
    35521 ttgttcgccg gatttagaat atctttcggt aaatcaacga ccacgggtcc aggacgacca
    35581 cttgccgcca gccagaaagc ctttttcagc acctgcggaa tgtcttccgt ttgcttaacc
    35641 agaaaactgt gtttaaccac cgggcgagaa atccccacca tgtcgcactc ctgaaaggca
    35701 tcgtagccta tcaacgaggt cgctacctgc ccggaaagga caactaaggg aatggaatcc
    35761 atataagcgg tggcaatgcc agtaatcgca ttggtcgccc ctggacccga cgttaccagc
    35821 acgacgccga cttccccggt cgcgcgcgcc agaccatcgg ccatatgcac cgccgcctgc
    35881 tcatgacgaa ctaaaacatg atcgatacca cccacggtat gcaatgcatc gtaaatatca
    35941 aggaccgcgc ctccgggata accgaatact tgtttaacgc cctgatcgat aagcgatcgg
    36001 acgaccatct cggctccaga caacatctcc atggcctgcc tcactgtttg acggaaaaaa
    36061 tgtgtaaaac acattaaccg tccgatcttg cttaagcaat tggcagcctg agacataaac
    36121 acggggtaat aagaataaac aggaggaaag gtgaaaagaa acgataaaat cctccattac
    36181 agaggattaa attgataata aatcggctga atcccacaac ttacagcaat ttctctattt
    36241 ttttaattaa tcctaaaata gcgagatcac acattcagaa taaaattcta tattcaataa
    36301 aaaatgcaga ataaaccaga cattaatgtc tgacctattc tgcattcagt tagcgtttgc
    36361 aaattgagac taattgctct tccatccact gatggccttt atcgcgcccg gcagcttcat
    36421 gccaggagag ataacaggtt ctgctgtttt gttttaacgg cagcggtaat acctgtaact
    36481 ctaaggattc agcgaactct tcagccagcc aacgcggcgc aatagcgacc agatgcgttt
    36541 gcgacaccac gctaagtacg ctcatcattg ccatgccctg atacgcgata ctggcttgct
    36601 tatctaccgt gtcataccag gtttgactaa atgacgcgaa acgatcgagc gaaaccgcag
    36661 catgttgttc gttataaaca tcatgtttca gtaacgggcc tttaattgtt ggatgatttt
    36721 tgctggctac caacaccatt tcatctttaa ataatggcac actggtaaat tcagggcgat
    36781 ggaagtcttc ataactaatc acaaactccg tttcctgata acgcagctga tgttcagtgt
    36841 tctgatttaa tgaagacttg aacataacat gtatatttgg cgcaatctgc tcaatgtgat
    36901 tataaatctg cgaggtcaga atgctgtcta acgggctgca aacacaaaga tgaaatacac
    36961 gttcactgct cgcgggttca aaacctgaac caggcaattc attttgtact agttgcaatg
    37021 cctgacgaac tgagccaaaa agttgaaatg cgcgagcagt cggttgaata ccacggccat
    37081 aacgaacaaa aagctcgtca ttaaacatca ccttcaggcg tgcaacagcg ttactgaccg
    37141 caggttgcga cattcccaga acatgagcgg cacgggtgat gttttgctcc tgcatcacgg
    37201 catcgaaaac ggttaataag ttgagatcga ccatgcgtag ctgtggcttg cttaactccg
    37261 ccgtctctgg atgatctgtt tgaacctctg gcatacttaa ctccactgtc acacttaact
    37321 ccctttccct tattggaatg cagagaaata atcctcgaga atatgattta tcatgcatat
    37381 aaaataagaa aaagga
//
LOCUS       DAZGAP010000019        36653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGAP010000019 DAZGAP010000000
VERSION     DAZGAP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.019"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..3799
                     /gene="yfaL"
                     /locus_tag="ACHLAM_000854"
     CDS             41..3799
                     /gene="yfaL"
                     /locus_tag="ACHLAM_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO7852424.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKANCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNYVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLESVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQVLDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFRLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVTPTPPPDPDPTPDPDPTPDPDPTPDPGPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDQAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGVWLDSWLQ
                     YAWFNNDVSEQDDGEDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWSTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     GVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3939..4661)
                     /gene="ubiG"
                     /locus_tag="ACHLAM_000855"
     CDS             complement(3939..4661)
                     /gene="ubiG"
                     /locus_tag="ACHLAM_000855"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO7852425.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4808..7435
                     /gene="gyrA"
                     /locus_tag="ACHLAM_000856"
     CDS             4808..7435
                     /gene="gyrA"
                     /locus_tag="ACHLAM_000856"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO7852426.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLIIQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7584..9272
                     /gene="yfaA"
                     /locus_tag="ACHLAM_000857"
     CDS             7584..9272
                     /gene="yfaA"
                     /locus_tag="ACHLAM_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO7852427.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALSHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTMLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9269..9892
                     /gene="yfaT"
                     /locus_tag="ACHLAM_000858"
     CDS             9269..9892
                     /gene="yfaT"
                     /locus_tag="ACHLAM_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO7852428.1"
                     /translation="MRHGLLALICWLCCVVTHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9916..14430
                     /locus_tag="ACHLAM_000859"
     CDS             9916..14430
                     /locus_tag="ACHLAM_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO7852429.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14431..16080
                     /gene="yfaQ"
                     /locus_tag="ACHLAM_000860"
     CDS             14431..16080
                     /gene="yfaQ"
                     /locus_tag="ACHLAM_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO7852430.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTFQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16085..16861
                     /gene="yfaP"
                     /locus_tag="ACHLAM_000861"
     CDS             16085..16861
                     /gene="yfaP"
                     /locus_tag="ACHLAM_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO7852431.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16935..18119)
                     /gene="atoB"
                     /locus_tag="ACHLAM_000862"
     CDS             complement(16935..18119)
                     /gene="atoB"
                     /locus_tag="ACHLAM_000862"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO7852432.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATKKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18150..19472)
                     /gene="atoE"
                     /locus_tag="ACHLAM_000863"
     CDS             complement(18150..19472)
                     /gene="atoE"
                     /locus_tag="ACHLAM_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO7852433.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPIMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19469..20119)
                     /gene="atoA"
                     /locus_tag="ACHLAM_000864"
     CDS             complement(19469..20119)
                     /gene="atoA"
                     /locus_tag="ACHLAM_000864"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO7852434.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20119..20781)
                     /gene="atoD"
                     /locus_tag="ACHLAM_000865"
     CDS             complement(20119..20781)
                     /gene="atoD"
                     /locus_tag="ACHLAM_000865"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO7852435.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(20977..22362)
                     /gene="atoC"
                     /locus_tag="ACHLAM_000866"
     CDS             complement(20977..22362)
                     /gene="atoC"
                     /locus_tag="ACHLAM_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO7852436.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22359..24185)
                     /gene="atoS"
                     /locus_tag="ACHLAM_000867"
     CDS             complement(22359..24185)
                     /gene="atoS"
                     /locus_tag="ACHLAM_000867"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO7852437.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24352..27201
                     /gene="rcsC"
                     /locus_tag="ACHLAM_000868"
     CDS             24352..27201
                     /gene="rcsC"
                     /locus_tag="ACHLAM_000868"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO7852438.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27287..27937)
                     /gene="rcsB"
                     /locus_tag="ACHLAM_000869"
     CDS             complement(27287..27937)
                     /gene="rcsB"
                     /locus_tag="ACHLAM_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO7852439.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(27982..29271)
                     /locus_tag="ACHLAM_000870"
     CDS             complement(27982..29271)
                     /locus_tag="ACHLAM_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7852440.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(29305..29559)
                     /gene="xisR"
                     /locus_tag="ACHLAM_000871"
     CDS             complement(29305..29559)
                     /gene="xisR"
                     /locus_tag="ACHLAM_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7852441.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(29567..29713)
                     /locus_tag="ACHLAM_000872"
     CDS             complement(29567..29713)
                     /locus_tag="ACHLAM_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852442.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(29734..29976)
                     /locus_tag="ACHLAM_000873"
     CDS             complement(29734..29976)
                     /locus_tag="ACHLAM_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852443.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPNELA"
     gene            complement(29969..30871)
                     /locus_tag="ACHLAM_000874"
     CDS             complement(29969..30871)
                     /locus_tag="ACHLAM_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852444.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(30868..31092)
                     /locus_tag="ACHLAM_000875"
     CDS             complement(30868..31092)
                     /locus_tag="ACHLAM_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852445.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(31109..31471)
                     /locus_tag="ACHLAM_000876"
     CDS             complement(31109..31471)
                     /locus_tag="ACHLAM_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO7852446.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(31464..32330)
                     /locus_tag="ACHLAM_000877"
     CDS             complement(31464..32330)
                     /locus_tag="ACHLAM_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO7852447.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGITVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(32354..32938)
                     /locus_tag="ACHLAM_000878"
     CDS             complement(32354..32938)
                     /locus_tag="ACHLAM_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852448.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(32925..33485)
                     /locus_tag="ACHLAM_000879"
     CDS             complement(32925..33485)
                     /locus_tag="ACHLAM_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852449.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQCLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(33893..34255)
                     /locus_tag="ACHLAM_000880"
     CDS             complement(33893..34255)
                     /locus_tag="ACHLAM_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852450.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGQKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITRCVNH"
     gene            complement(34430..34630)
                     /locus_tag="ACHLAM_000881"
     CDS             complement(34430..34630)
                     /locus_tag="ACHLAM_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852451.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(34627..34818)
                     /locus_tag="ACHLAM_000882"
     CDS             complement(34627..34818)
                     /locus_tag="ACHLAM_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852452.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(34820..35197)
                     /locus_tag="ACHLAM_000883"
     CDS             complement(34820..35197)
                     /locus_tag="ACHLAM_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7852453.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(35283..35942)
                     /locus_tag="ACHLAM_000884"
     CDS             complement(35283..35942)
                     /locus_tag="ACHLAM_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7852454.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            36282..36575
                     /locus_tag="ACHLAM_000885"
     CDS             36282..36575
                     /locus_tag="ACHLAM_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852455.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT         8868 a         8898 c         9561 g         9326 t
ORIGIN      
        1 ttaaatacgc catcttatag aaaatgatgg ttagatgaat atgcggatta tctttctacg
       61 caaggagtat ttatctttac tcccgtcaat gattgcatct cttttctctg gtaacggtgc
      121 cgcagcggtc atcgattcat gccagggata tgatatcaaa gcgaactgtc aggccagcag
      181 gcaaagcctt tcgggcaata tgcaggactg gagcatcgcg gatgggcaat ggttgatttt
      241 ttcgggtatg gccaataatg ccagcggtgg cgcagtattt ttgcagcaaa gtgctgaatt
      301 tacgatttca ccacaaaatg aaacagggat gaccctgttt gctgataact cggttagtgg
      361 cgaatataat aatggtgggg ctatctttgc taaagaaaac tcaacgataa atcttgcgaa
      421 tgttattttc gacagtaatg ttgcaggagg ctatggcgga gcaatctatt ctgccggtac
      481 caatgatacc ggtgccgccg atttacgtgt cactaacgcc gtatttcaca ataacatcgc
      541 taatgacggc aaaggcggtg caatttatac catcaataat tacgtctatt taagtgatga
      601 tgcttttaat aataaccagg catatacatc aacaagttat agtgatgggg atggcggcgc
      661 tattgatgtt acggataata gtacggataa cacacatctt tcaggaaaaa cgataattaa
      721 taatacctcc tttaccaata actatgcaga aggttatggc ggagcaattt ataccagcag
      781 caccacatcc ccgtatctta ttgatatttc tgttgatgat aattatgacc aaaacaatgg
      841 tgtgatgatt gatgaaaata acagcgcctc gggatatgac cattcggcga cggcagcggc
      901 ggggggcttt atgtatatcg gtcatagtgt ggctgaattt aacattgccg ccgataaaac
      961 gctggtaata ggcaatacca gcaacgatgg cgcgattgat tctcttgccg gaacgggagt
     1021 catcgttaaa gagggagcgg gagagttagt cctcaatgcc gacaataatg cgtttaccgg
     1081 cgagataagt atccagaatg gcgaggtgac gttggggcgc agcgatgagt taatgaatgt
     1141 cggcgacacg cactgtcaga gcgatccgca ggattgcttt ggtctgatgg tcggcagcac
     1201 tgttcattct gagtatcagg cagaactgaa tgttggcaat acacaacaaa cgtttgtgca
     1261 ctcattaacc ggttttgcta atggcatttt gaatatcgac gcaggcggca atgttaccgt
     1321 aaaccagggc ggtttttccg gctcgattca gggtgaagga cagttgacgg tagcgcaaga
     1381 tggcagctat ctcctgacag gggcgcagtc gatggcatta accggcgata ttgtggtgga
     1441 agataacgcc gtattgtcgc tagcggggaa tcaggccgat ttacgggcaa tgcagagcga
     1501 tccgcaatcg atcgtgctaa atggcggagt attggatctc tctgatttca ccacctggga
     1561 tggcgatagc tcatacaatg acggtctgca aatcagcggc agcgggggga cagtgattgg
     1621 cagtaatgat gtcgttgata tcagcagtgg cgatgattta catattggcg gcagtgatgc
     1681 cagccagaat ggcgtttatg tcgtcattaa tgcgggggat cagcgcgtaa cgctggcgaa
     1741 taacaacggt tatctcggta atacgcaaat cgcctccggt acgcttgaag tcagcgataa
     1801 ttcgcaattg ggggacacaa gttacaaccg ttcagtgatc ttcacagatc cccaacaaca
     1861 cagtgaaatg gatgtgacga ctgatgttga cactcgctcg gctacaacag gtcagggcag
     1921 gaatattgaa atgcgcgctg atggcgaaat acacgttgag gatggcgtag atacgcaatg
     1981 gggcgggttg atggcggata gtaccgggca acaactggag agcgtaagca cgttgactaa
     2041 aagtggcggc ggtacgctgg agttgaccgc cagcggcact gcgacgtctg cggtacgtgt
     2101 ggaggacgga acgctaaaag gtgaagcgga gaatattatt ccttatgttt cttcactgtg
     2161 ggtgggggaa gacggtgttt ttgaaaccgg gcaaaatcag gatattcgct caatcgatgc
     2221 cacctctggc ggcgatatcg acataaccga tggtactgtg ttgcgattga cgcagcagga
     2281 tacgaaccag gtgctggatg cctcgctgtt tagcggcgac ggtacgttgg tgaatgccac
     2341 cgatggcgtg acgctgacag gcgagcttaa taccaacctt gaaactgaca gcctgactta
     2401 tctttccgac gtgacggtta atggcaatct gaccaatacg tccggtgcga ttagcctgca
     2461 aaatggcgtc gctggcgaca cgctgacggt aaatggtgat tataccggcg gcggtacgct
     2521 actgctcgac agcgaattaa acggcgatga ctcggcaagt gaccaactgg tattgaacgg
     2581 taatactgct ggcaacacgg ccgtggtaat taaccccatt acgggtattg gtgagccgac
     2641 atctacaggt attaaagtgg ttgatttcgc agctgatccc acgcagtttc aaaacaatgc
     2701 gcagttccgt ctggcaggaa gcggctacgt caatatgggg gcgtatgact atacgctggt
     2761 ggaagataac aacgactggt atctgcgatc gcaagaagtc actccgacgc cgccgcccga
     2821 tccggacccg actccagatc ctgatcccac gccggatccc gacccaaccc ccgaccctgg
     2881 acctacgcct gcttaccagc cggtgttgaa tgccaaagtt ggtggatatt ttaataacct
     2941 acgtgcggca aatcaggcat ttgtgatgga gcgacgcgat caggctggtg gcgatggtca
     3001 gacgctgaat ttacgcgtta tcggagggcg ttatcattac acggcggcag ggcagttggc
     3061 tcagcatgaa gacacttcta cggtgcagct tagcggcaac ctgtttagcg ggcactgggg
     3121 tgatgatggc gagtggatgc ttgggattgt tggtggctac agcgataacc agggcgacag
     3181 ccgctcgaat atgaccggaa ctcgcgccga taaccagaac cacggttatg ccgttgggct
     3241 gacctcaagc tggtatcagc acggtaatca gaagcagggg gtctggctgg atagctggct
     3301 gcaatatgcg tggtttaaca atgatgtttc cgaacaagac gatggcgagg atcattacca
     3361 ctcgtcgggg attatcgcct cgctggaagc cggttatcag tggttaccgg ggcatggtgt
     3421 ggtgattgaa ccgcagacgc aggtgattta tcagggcgtg cagcaggatg attttaccgc
     3481 cgctaaccat gcgcgcgtgt cacaatcgca gggtgatgat attcagacgc ggctgggttt
     3541 acacagcgaa tggagtactg ctgttggtgt cacgccaacg ttagatctga attattatca
     3601 cgatccccat gcgacggaaa ttgaagagga tggcagcact atcagtgacg atggggtgaa
     3661 gcaacggggt gaaataaaag tgggagtcac gggcaatatc agtcagcgag tgtccctgcg
     3721 cggcagcgtg gcgtggcaga aagggagtga tgattttgcc cagacggcag ggtttttgtc
     3781 gatgacggtg aaatggtaat cgctgtctga ccggcacttt tgccggatgc ggcgtaaacg
     3841 ccttatctgg cctacgggag catttgtagg cctgataaga cgcggcaagc gtcgcatcag
     3901 gcattggcac ggttgtcgga tgaatccaaa caacaacctc acttattctg cgtgtgcagc
     3961 atatagttca catccacgcc ggggccgagc ttaaaggaat tagtgatcgg gttgtaatgc
     4021 agcccggtca tatgccgctc ttttaaactg gtctgatcca cccagcccag caattctgcc
     4081 ggtttaataa acttcttcac atcatgcgtg cctttgggca ccatgcgcaa aatatattca
     4141 gcaccaacca ccgccatcag ccatgacttg ccgttgcggt taagcgtcga gaaaaagaca
     4201 tcgccgcctg gtttcaccag ttgcgcacag gctctgacca ctgactgcgg atcggggacg
     4261 tgctccagca tctccatgca ggtcactaca tcatactgcc cggcatgttt tgccgcgtgc
     4321 tcttccacgg tttcctgtac gtactctacc tgaatgccgc tttccagcgc gtgcaacttt
     4381 gccacctgca atggctcaaa gcccatatcc agaccggtca ccgtcgcgcc ttcgcgcgcc
     4441 atactctcgg ccagaatgcc gccgccacaa ccgacatcga gcaccttttt gccaaataaa
     4501 ccgccagcac gctcggcaat atagcccaga cgcagcgggt taatgcggtg cagcggtttg
     4561 aactcacctt caagatccca ccagcgggag gcgacagctt caaatttagc gatctcttcg
     4621 tggtctacgt tatggtttac cggcgatttt tcggcattca ttggcacttc tactccgtaa
     4681 ttggcaagac aaacgagtat atcaggcatt ggaggtgaat aaagcgtata ggtttacctc
     4741 aaactgcgcg gctgtgttat aatttgcgac ctttgaatcc gggatacagt agagggatag
     4801 cggttagatg agcgaccttg cgagagaaat tacaccggtc aacattgagg aagagctgaa
     4861 gagctcctat ctggattatg cgatgtcggt cattgttggc cgtgcgctgc cagatgtccg
     4921 agatggcctg aagccggtac accgtcgcgt actttacgcc atgaacgtac taggcaatga
     4981 ctggaacaaa gcctataaaa aatctgcccg tgtcgttggt gacgtaatcg gtaaatacca
     5041 tccccatggt gactcggcgg tttatgacac gatcgtccgt atggcgcagc cattctcgct
     5101 gcgttacatg ctggtagacg gtcagggtaa cttcggttcc atcgacggcg actctgcggc
     5161 ggcaatgcgt tatacggaaa tccgtctggc gaaaattgcc catgaactga tggccgatct
     5221 cgaaaaagag acggtcgatt tcgtagataa ctatgacggc acggaaaaaa ttcccgacgt
     5281 catgccaacc aaaattccta acctgctggt gaacggttct tccggtatcg ccgtaggtat
     5341 ggcaaccaac atcccgccgc acaacctgac ggaagtcatc aacggttgtc tggcgtatat
     5401 cgatgatgaa gacatcagca ttgaagggct gatggaacac atccccgggc cggacttccc
     5461 gacggcggca atcattaacg gtcgtcgcgg tattgaagaa gcttaccgta ccggtcgcgg
     5521 caaggtatat atccgcgccc gtgctgaagt ggaagttgac gccaaaaccg gacgtgaaac
     5581 cattatcgtc cacgaaattc cgtatcaggt aaacaaagcg cgcctgatcg agaagattgc
     5641 ggaactggta aaagaaaaac gcgtggaagg catcagcgcg ctgcgtgacg agtctgacaa
     5701 agacggtatg cgcatcgtga ttgaagtgaa acgcgatgcg gtcggtgaag ttgtgctcaa
     5761 caacctctac tcccagaccc agttgcaggt ttctttcggt atcaacatgg tggcattgca
     5821 ccatggtcag ccgaagatca tgaacctgaa agacatcatc gcggcgtttg ttcgtcaccg
     5881 ccgcgaagtg gtgactcgtc gtactatttt cgaactgcgt aaagctcgcg atcgtgctca
     5941 tatccttgaa gcattagccg tagcgctggc gaacatcgac ccgatcatcg aactgatccg
     6001 tcatgcgccg acgcctgcag aagcgaaaac tgcgctggtg gctaatccgt ggcagctggg
     6061 caacgttgcc gccatgctcg aacgtgcggg cgatgatgct gcgcgtccgg aatggctgga
     6121 gccagagttc ggcgtgcgtg atggtctgta ctacctgacc gaacagcaag ctcaggcgat
     6181 tctggatctg cgtttgcaga aactgaccgg ccttgagcat gaaaaactgc tcgacgaata
     6241 caaagagctg ctggatcaga tcgcagaact gctgcgtatt cttggcagtg ccgatcgtct
     6301 gatggaagtg atccgcgaag agctggaact ggttcgtgaa cagttcggtg acaaacgtcg
     6361 tactgaaatc accgctaaca gcgccgacat caacctggaa gatctgatca tccaggaaga
     6421 tgtggtggtc acgctttctc accagggtta cgtgaagtat cagccgcttt ctgaatatga
     6481 agcgcagcgt cgtggcggga aaggtaaatc tgccgcacgt attaaagaag aagactttat
     6541 cgaccgactg ctggtggcga acacccacga ccatattctg tgcttctcca gccgtggccg
     6601 cgtctattcg atgaaggtct accagttacc agaagccact cgtggcgcgc gtggtcgtcc
     6661 gatcgtcaac ctgctgccgc tggagcagga cgaacgtatc actgcgatcc tgccggtgac
     6721 cgagtttgaa gaaggcgtga aagtcttcat ggcgaccgct aacggtactg tgaagaaaac
     6781 cgtcctcacc gagttcaacc gtctgcgtac cgccggtaaa gtggcgatca aactggttga
     6841 aggcgatgag ctgatcggcg ttgacctgac cagcggcgaa gacgaagtaa tgctgttctc
     6901 cgccgaaggt aaagtggtgc gctttaaaga gtcttctgtc cgtgcgatgg gctgcaacac
     6961 caccggtgtt cgcggtattc gtttaggcga aggcgataaa gtcgtctctc tgatcgtgcc
     7021 tcgtggcgat ggcgcaatcc tcaccgcaac gcagaacggt tatggtaaac gtaccgcagt
     7081 ggcggaatac ccgactaagt cgcgtgcgac gaaaggggtt atctccatca aggttaccga
     7141 acgtaacggt ttagtggttg gcgcggtgca ggtagatgac tgcgaccaga tcatgatgat
     7201 caccgatgcc ggtacgctgg tacgtactcg cgtttcggaa atcagcatcg taggccgtaa
     7261 cacccagggc gtgatcctca tccgtactgc ggaagatgaa aacgtagtgg gtctgcaacg
     7321 tgttgctgaa ccggttgacg aggaagatct ggataccatc gatggcagtg ccgcggaagg
     7381 ggacgatgaa atcgctccgg aagtggacgt tgacgacgag ccagaagaag aataatttta
     7441 cttcttcatg ccaaaaggga gctatctccc ttgtttgaat tgaaaagtcc aggctgcaaa
     7501 gtctgggctt ttgtcgtatt agggcgcggt aaagttttgc tgtgctcgta aaaaatggct
     7561 ggctttacac aaggaatgtg gcaatgagtg gtgaaaaaaa ggcgaaaggc tggcggttct
     7621 atggtcttgt aggtttgggc gcaatagtac tgctttccgc tggcgtctgg gcgttgcaat
     7681 atgctggcag tgggccagaa aaaacgttgt cgccgctggt ggtgcacaac aatctgcaaa
     7741 tcgatctcaa tgagccggac ctctttctcg acagcgactc tctgagccag cttcccaaag
     7801 acctccttac cattccgttt ctccacgatg ttctgagtga agatttcgtt ttctattatc
     7861 agaatcatgc cgatcgtctg ggcattgaag gcagcattcg ccgcattgtc tatgaacacg
     7921 atctcacgct gaaagataag ctcttttcgt cactcttaga tcagcccgcg caggcggcgc
     7981 tatcgcacga taaacaaggc catctttcac attacatggt gctgatccag cgcagtggtt
     8041 taagcaaact gttggagcca ttgttgtttg ccgctaccag cgacagccag ttaagcaaaa
     8101 cggaaatcag tagtattaag ctaaatagtg aaaccatccc ggtttatcag ttgcgctata
     8161 acggcaataa cgccctgatg ttcgcgactt atcaggacaa gatgctggtg ttttccagta
     8221 cggatatgct gtttaaagat gaccagcagg ataccgaagc cacggcgatc gcaagtgatt
     8281 tgttgagcgg taaaaaacgc tggcaagcca gctttggcct ggaagagcgt gctgctgaaa
     8341 aaacgccagt acgccagcgt attgttgtca gcgccaggtt gctggggttt ggctaccagc
     8401 gattaatgcc ttcttttgct ggcgtacgct tcgaaatgag caacgacggc tggcatagct
     8461 ttgtggcgtt aaatgatgaa tccgccagcg tagatgccag tttcgatttt acgccggtat
     8521 ggaacagtat gcctgccggg gccagcttct gtgtggcggt gccgtattcg catggtattg
     8581 ccgaagagat gctttcgcac atcagccagg aaaacgacaa gctgaacggg gcgttagatg
     8641 gtgcagccgg gctgtgctgg tatgaagact caaaattaca aaccccgcta tttgttggtc
     8701 aatttgatgg ctcagccgaa caggcacaac tgcctgggaa actgtttacg caaaatattg
     8761 gtgcgcacga aagcaaagcg ccagaaggtg ttttgccggt aaaccagact cagcagggcg
     8821 aagcgcaaat ctggcgtcgc gaagtgagtt cccgatacgg ccagtatccg aaagcgcagg
     8881 cggtgcaacc cgatcaatta atgtcggatt attttttccg cgtgtcgctg gcgatgcaaa
     8941 acaagacgat gcttttctcc ctcgatgaca cgctggttaa taacgcgctg caaacactga
     9001 ataaaacccg cccggcaatg gtggatgtga tacccactga cggcatcgtt ccgctctata
     9061 tcaatccaca aggcgtggcg aaactgctgc gtaacgaaac gctgaccagt ttgccgaaga
     9121 atctcgaacc ggttttttat aacgccgcac aaactttatt aatgccgaag ctggacgctt
     9181 tatctcaaca accgcgttat gtcatgaagc tggcccagat ggaacccggt gccgcctggc
     9241 agtggctacc cataacctgg caaccgctat gagacacggg ctgctggcgc tgatttgctg
     9301 gctgtgttgt gttgttaccc atagcgaaat gctgaatgtc gaacaatccg ggctgtttcg
     9361 cgcctggttt gtacgcattg cacaggaaca gctccgccag ggtccaagtc cacgctggta
     9421 tcagcaggat tgtgcgggcc tggtgcgatt tgcagcgaac gagacgctga aagttcacga
     9481 cagtaaatgg ctaaaaagta acgggttatc taaccagtat ttgccgccag agatgacgct
     9541 aacacctgaa cagcggcaac tggcgcaaaa ctggaatcag gggaacggga aaaccggccc
     9601 ctacgtgacc gcaattaatt tgattcagta caacagccaa tttattggcc aggacataaa
     9661 ccaggcgctg cctggcgata tgattttttt cgatcagggc gatgcccagc acttaatggt
     9721 ctggatgggg cgttacgtca tctaccacac cggaagcgcc acgaaaactg acaacggaat
     9781 gcgcgcagtc agtctgcaac aacttatgac atggaaggac acccgatgga tacccaacga
     9841 ttccaatccc aatttcattg gcatttatcg tttaaatttt ctggcgcgat agcagcgtgc
     9901 ttgttcctgt ctcttgtggg aacagggctt gccaatgctg atgattcgct tccttccagc
     9961 aactatgcgc cgcccgccgg gggaacattc tttttgctcg ctgacagcag ttttagcagc
    10021 agtgaagagg cgaaagtgcg actggaagcg ccggggcgtg attatcgacg ctatcagatg
    10081 gaagagtacg gcggcgtgga cgttcgcctg tatcgtattc ctgacccgat ggcatttttg
    10141 cgccagcaga aaaacctgca tcgcattgtg gtgcaaccgc aatatttggg cgacgggctg
    10201 aacaatacgc tgacctggct gtgggataac tggtacggca aatctcgccg cgtgatgcag
    10261 cgtacttttt cttctcagtc tcggcagaat gtgactcagg cgttacccga attacagctc
    10321 ggtaatgcca ttattaaacc ttcccgttat gtacagaaca accagttttc accgctgaaa
    10381 aaatatcccc tggtggaaca gttccgttac ccattatggc aggctaaacc ggtcgagccg
    10441 cagcaagggg taaaactgga aggcgcatcc agcaatttca tctcgccgca gccgggcaac
    10501 atctatattc cgctaggcaa gcaagagccg gggctgtacc tcgtcgaggc gatggtaggt
    10561 gggtatcggg cgacgacggt ggtgtttgtt tccgataccg tggcgcttag caaagtgtca
    10621 ggtaacgagc ttctggtgtg gaccgcgggt aaaaaacagg gggaagcgaa gccgggatca
    10681 gagatcttgt ggactgacgg tcttggcgtg atgacccgcg gtgtgaccga tgacagcggt
    10741 accttgcagt tacaacatat atcgccggaa cgttcataca ttctgggtaa agatgctgaa
    10801 ggcggcgttt ttgtctccga gaattttttc tacgaaagcg aaatctacaa cacccgcttg
    10861 tatatcttta ccgaccgccc tctgtatcgt gcaggcgatc gtgttgatgt caaagtgatg
    10921 ggccgcgagt tccacgaccc gttgcattca tcccccatcg tcagcgcccc ggcgaagctt
    10981 tcagtgctgg acgccaacgg cagtctgttg caaaccgtca atgtcacgct tgatgcgcgt
    11041 aatggcgggc aggggagttt ccgcctgcca gaaaatgccg tcgccggagg ttatgagtta
    11101 cgcctggctt accgcaaaca ggtctatagc agcagttttc gcgtggcaaa ctacatcaag
    11161 ccacatttcg agattggttt agctctcgac aaaaaagagt tcaaaacagg cgaagcggtg
    11221 agcggcaaac tgcaactgct ttacccggat ggagagccgg taaaagatgc ccgcgtgcag
    11281 ttaagtttgc gcgctcagca attatcaatg gtcggtaacg atttgcgtta tgccggacgt
    11341 tttcccgtgt cgctggaagg cagcgaaacg gtgtccgatg atagcggtca tgtggcttta
    11401 aatctccccg ccgccgataa accgagccgc tatttgttaa ccgtctccgc cagtgacggc
    11461 gcggcgtatc gcgtcaccac caccaaagag atcctcattg aacgcggcct ggcgcattac
    11521 tctttaagta ccgccgcaca atacagtaat agcggcgagt cggttgtgtt ccgttatgcc
    11581 gcgctggaat cttcaaaaca ggttcctgtt acgtatgaat ggttgcgtct cgaagaccgc
    11641 acgagccata gcggagatct accgtcaggc ggcaaatcct ttaccgtcaa tttcgataaa
    11701 cctggcaact acaatctgac attacgcgat gaagacggct taattctcgc tgggttaagc
    11761 catgcagtca gcggtaaggg cagcacggcg catacgggta cggtagatat cgtggcggat
    11821 aaaacgctgt accagccagg cgaaaccgcg aagatgctga ttaccttccc ggagccaatt
    11881 gatgaagcat tattgacgct ggaacgcgat cgcgttgaac agcagtcgct gctatcgcat
    11941 ccggcaaact ggctgacgtt acaacgttta aacgataccc agtatgaagc ccgcatacca
    12001 gtgagcaatt cctttgcgcc taacatcact ttttcggtgc tgtatacccg taacggtcag
    12061 tacagttttc agaacgccgg gatcaaagtt gccgttcccc agctggatat ccgggtgaaa
    12121 acggaaaaaa cccattacca gcctggtgaa ctggtcaatg tcgaattaac ctcgtcgctg
    12181 aaaggtaaac ctgtttctgc gcagctaacg gttggtgtgg tcgatgaaat gatctacgcg
    12241 ctgcaaccgg aaatcgcgcc gaatatcggc aaatttttct atccgctggg gcgtaacaat
    12301 gtgcgtacca gctccagtct gtcgtttatc agctacgacc aggcgctctc cagcgagccg
    12361 gttgcgcctg gcgcgactaa ccgcagcgag cggcgagtaa aaatgcttga acgtccacgg
    12421 cgtgaagagg tggataccgc ggcatggatg ccgtcactca caaccgataa acaaggcaaa
    12481 gcgtatttca cgttcctgat gcctgattcg ttaacccgct ggcgcatcac cgcgcgtggg
    12541 atgaacggcg acgggctggt cgggcagggg cgtgcttatc tgcgttcgga aaaaaatctc
    12601 tacatgaagt ggagtatgcc aacggtgtat cgcatgggcg acaaaccggc agcaggactg
    12661 tttatcttca gtcagcagga taacgaaccg gtggcgctgg tgactaaatt tgcaggtgct
    12721 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct gacgcagaat
    12781 attcagcaat ctggcttgtt aagtgcagaa ctgcaacaaa atgggcaagt acaggacagc
    12841 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaacgtc
    12901 atgctcggcg gtggcgataa cgcgctgatg ttgcccgagc aggcgagcaa tatccagcta
    12961 caaagtagcg aaacgccgca ggagattttc cgtaacaatc ttgatgcgtt agtcgatgaa
    13021 ccgtgggggg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
    13081 tcacttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
    13141 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
    13201 ggcaatggcg acgccttcct gacggcatgg gcatggtacg ccgactggca ggccagccag
    13261 gcgctcggcg taacgcagca accggattac tggcaacata tgctcgacag ttatgccgag
    13321 caggcagata acatgccgat attgcatcgc gcgctggtgc tggcgtgggc acaggagatg
    13381 aatctaccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
    13441 aaaactgaag atttctctga ggaagacacc agcgatatca atgacagcct gatcctcgat
    13501 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
    13561 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
    13621 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
    13681 acccaggcgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
    13741 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
    13801 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
    13861 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
    13921 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
    13981 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
    14041 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
    14101 caggatgccg ttctgcgcta cggtcaggtg gaagtaccgc tcccgccagg ggctgacgtt
    14161 gagaggacta cgtggggcat ttcggtcaat aaaccgaacg ctggaaaaca gcaggggcag
    14221 ttgctggaaa aagcgcgaaa tgaaatgggc aaactggcct atatggtgcc ggtgaaagaa
    14281 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
    14341 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcac agcaaagtgt tgcggcaggg
    14401 agcgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
    14461 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
    14521 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcggcatta
    14581 cctgacacgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
    14641 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
    14701 tattgttgtc aggctgggga gagtataacg cgcgataccg cgctggtacg ttcctgtggg
    14761 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atattggcaa
    14821 cagcgtcagg cacctgcgtg gttagcgtct cttactacgc tgaagccaga aacctcggta
    14881 acggttaact cgctgcttga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
    14941 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggcagt
    15001 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgtcagggt
    15061 ggttttgccg gctggttaac cgatggtaca ccgctgtggg tgactggcag tggcaccagc
    15121 aaaacggtac tgacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcaa
    15181 gttgccagtg ggcaatgtgt cctggtggag ctgtttgccc gctatccgct gaagaaaatc
    15241 acggcggaaa agagttcgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
    15301 acgtttgcca atggtaacca cattatgttt gtcagccacg gtgagacgac cttactgagc
    15361 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
    15421 ctggatcgtg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
    15481 cgcacttttt tacagcaaaa cgccaatcgg gaaggcgatt gcctgaccat ccctgacagc
    15541 agcgccacgc agcgtgtttc tgcttcgcca gctaccacgg gggcgcgaac gatgaccgcc
    15601 tggacgcagg atcttatcta tgcgggcgat ccggttcatt atcacggcag tcgcgctacc
    15661 gaagggacgc tttcctggcg gcaagccacg gcccaggcgg ggcagggaga gcgttacgat
    15721 cagatactcg cgtttgctta tcccgacaat agccttagcc gctggggagc gccacgctca
    15781 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
    15841 cgtatattac aaggtgagac ggggtacaac gaaccagacg tgtttgcggt ttgccgttta
    15901 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
    15961 acgtttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
    16021 cccaccgggc ttgatgagaa ctatatcgaa acgcttaccc gacaattatt aatggactaa
    16081 tgctatgcga aagatttttc ttccgttgtt actggtggcg ctttcgcctg tcgctcacag
    16141 tgaaggtgtg caggaagtcg agattgatgc accgctctct ggctggcatc cggcagaggg
    16201 tgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
    16261 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
    16321 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
    16381 tggttcattt gcacgccctt atattttcac ggaaggcagc aacagcgtgc aggtcatcag
    16441 cccggatggg caaagccggc aaaaaatgca gttttactca acgccgggta caggaacgat
    16501 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
    16561 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac acggtgctga aaaacagtgg
    16621 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcgcc
    16681 gattcacgga cgttatcagg tgtatatcaa ctactatggc ggacgcagcg aaacggaatt
    16741 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
    16801 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
    16861 attaatgggg ttaccttcgc cgtcgatgtt caagaaaaca cccgataact ttcgctatcg
    16921 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
    16981 atgcatagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
    17041 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
    17101 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcaaggaa ctgtgcagca
    17161 aatgcttcat tagcctcaat gagatcaata tccgccagtt gcagccccgc cagttgtaac
    17221 gcttttttcg tggcaggtac tggccccata cccatcaatg cggggggcac gccaccgctg
    17281 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
    17341 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccggc ggtgactgtt
    17401 cctgctttat cgaaggccgg gcgcaatgcg cctaacgctt cagtcgtaga atccgctttc
    17461 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
    17521 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
    17581 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
    17641 atccccatat gataaccatg ggtggcgcac atcagaccat cgcgcaggat tacgtcataa
    17701 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
    17761 aaactcatat tttccatacc ccccgccaca atgctctgcg cctgacctgc ctgaattgcc
    17821 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
    17881 cacaccgttt cagccagtcc gctttttagc agcgcctgac gtgccggatt ttgccccagt
    17941 ccggcttgca acacgttacc cataatcact tcatcaacgt gtagtgaatc gatttttgca
    18001 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
    18061 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
    18121 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
    18181 acgccggaga agagcaaggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
    18241 gcgattgcca gtgctggcag cgcccagaac ggttgtgcca tgttcatcca ttgctcgccg
    18301 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggcatc
    18361 acgaacggcc cctgaataac ccagtgcccg ccgccagacg gaacggcgaa gttaatcagt
    18421 gcagaactaa aaaaggtcat gatcgggaag gtgtctttgt tcgcaacatt gatgaagaat
    18481 tcggtaatga gtccgcccag accggaatgc tccatcatca gttggatccc agcgtagaag
    18541 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
    18601 gccattggcg ttttatgtag cagcagaccc gcaatcataa acatcaggtt gacggtattg
    18661 atggtgatgt tgaagccatg ttcgctgaag tacatcgcaa ggtaagcgat accgagtgcg
    18721 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
    18781 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt tcggatcgat actcaccacg
    18841 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgccaca
    18901 gtgatgaaaa tattaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
    18961 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgagccaga gaaaccgcca
    19021 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggt
    19081 actcgccggg cgacttcacg ggcgaacatt gcaccgacaa ccaaaccaaa tccccagttg
    19141 atgacacaag cgactgaacc gaagaaagta accagcatga cgccctgtac gggcgtcttt
    19201 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cggagctggc aagggcatga
    19261 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccatca
    19321 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
    19381 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccaccg gctgacaaaa
    19441 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
    19501 cggcaacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
    19561 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
    19621 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gcggcgcaaa atttttgctg
    19681 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgtgacccg gtcaccagat
    19741 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
    19801 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccgcggatta
    19861 gcgcaaatga catggcgcta tcaaacatgg ctgcgccggg taaaatgccg cacggttgtc
    19921 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
    19981 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
    20041 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
    20101 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
    20161 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
    20221 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
    20281 ataggtaagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
    20341 cagcgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
    20401 tacgacggtg ccgacaccag ttggggtgag aaaaccacca agtccagctc cgccacagcg
    20461 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
    20521 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
    20581 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
    20641 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
    20701 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
    20761 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
    20821 gggaatagca aggggagtgc cagaatgaga ttattgaatg ttgttattta actaattgaa
    20881 tagaaaggaa taaattagat ggggattcat aaggagaatg agcggctatg cagaaaattg
    20941 cgcactgtgc aaatttctgc atagcaagtt ttggcgttat acatccgccg gatcgatacc
    21001 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta acgcagtgcg
    21061 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
    21121 aatttcctct tttaaattac gctcaccgac aggggctgtt tttgcctcac cagcattaca
    21181 gactggctga cgaatctgcg gcggaagatc ctcagagaaa atgatcgggc ctgagttcat
    21241 cacgacggcg cgttcaatga cgttggaaag ctctcgaata ttacccggcc atgaccaggc
    21301 ggtaagcagc gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
    21361 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
    21421 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
    21481 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
    21541 ggtctgatga ccgccaatcc gttcaaattc ccgttcctgc agaatgcgta gtaatttggc
    21601 ttgcagtacg agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggcacg
    21661 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
    21721 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
    21781 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
    21841 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
    21901 atccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg tgctcagtgc
    21961 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
    22021 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggccc cgcagcgtag
    22081 cgcttcgacg gcggtttcca cttcagcata ggccgtcatc agaataacgg gtgtccgggt
    22141 ctcattactg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
    22201 catcaacacc acgtcagggt gaatatcagc aaacaggtgt aatgctgtgc gtccgttgtt
    22261 cgcacaatgt gtttcgaaac cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
    22321 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
    22381 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
    22441 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
    22501 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
    22561 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggttcgaatg
    22621 cgaattttcc ctcgtgcact gatagcctgg acggcattga tcaggatatt cagtagtacc
    22681 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    22741 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    22801 gcattgaggc tgacttgttg ccattgactg tggcgtgggc gtgaaaattc gagcaattgc
    22861 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    22921 attgggtcac gggtttgttg gcgcaagatc tgtacataac cacgaatagc tgttagcgga
    22981 ttacgtactt catgcgccac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    23041 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    23101 cctatcattt caccgtgcgt gttatgaata cgactggtcg tgacactgag ttcaatggtg
    23161 cggtcacggc ctggaaaact gatctccagt gctacatgtt cggtgccatg ttccagcgta
    23221 tccagtacgg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    23281 agttcatgac gttgatagcc agtgatgact tctgctgctg ggttcatggt ggttacatca
    23341 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    23401 gttcgcgttt cacgcagtgc ctgggcgagg ttgttaacac tctgactgat ttgccccatt
    23461 tcaccgggca attgtggtaa tcgagtggga atattttgag ccagagtcga gaggccatcg
    23521 gtgatgatat ctatattggc gctcaggcga cgggagaaaa ggacaatcaa cagcaggctt
    23581 atcagcaaac cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    23641 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    23701 atgggaatca ttgaattcaa aatatcgccg cgcacctgtc tgcctgaata aaccaaaggg
    23761 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gcccacatta
    23821 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcgtttt gttgtaataa
    23881 ccagcgccga tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    23941 tttaatgcgc ggatacgctc ctcacgcggt aagtcgatgt agagatcgta gcgattgcct
    24001 agcgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    24061 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    24121 aggattgcca tcaggatcat ttgattacgt aagcggcgtg gataaatcca cttcatataa
    24181 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    24241 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    24301 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    24361 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    24421 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    24481 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    24541 caacgcacct ctgatgtgat gaaagagctg aagtacatag ctgaaaatcg cttatcggca
    24601 gaaaacggtg tgctttcccc gcgtggacga gaaacgcaga cggatgtgcc tgcgtttgaa
    24661 ccgctatttg ctgactctga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    24721 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatcttaac
    24781 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    24841 ccagtggaac gcgataccgc gttaaaagct ttgcatgaac gcattaataa atatcgaaat
    24901 gcaccacaag atgatagtgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    24961 gtcgggtatt tttacgcctt gacgccagtt tatctggcga accggttgca ggcgcttttg
    25021 ggtgtcgagc agaccatccg gatggagaac tttttcttac ctggtacgtt gccgatgggg
    25081 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagcaaa
    25141 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    25201 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    25261 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    25321 gtgcttgccg gagctgcatt gtttactctc gcgcggatgt acgagcggcg tattttcatt
    25381 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agtttaatcg caagattgtc
    25441 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    25501 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acggctgaca
    25561 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaac
    25621 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    25681 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    25741 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    25801 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    25861 gtcgatcgtc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    25921 gatattctcg acttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    25981 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    26041 cagctaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    26101 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    26161 ggctgtatag ttctgcatgt tcgcgctgat ggcgattatc tctctatccg tgttcgcgat
    26221 accggcgtag ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    26281 ggaacgggcg tacagcgtaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    26341 ctgatcagca tgatggacgg cgacatctcg gtagattcag aaccgggaat gggcagccag
    26401 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    26461 ttgagtggta aacgttgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    26521 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    26581 gaagatgtgt taatcactga cgaggtagtg agcaaaaaat ggcagggtag agcggttgtg
    26641 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccaggaga gtgggtacac
    26701 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    26761 gagagcgacg atcctgctaa cgctctgccg ttaacggaca aagcggtcag cgataatgac
    26821 gatatgatga ttctggtcgt ggatgatcat ccgattaacc ggcgtctgct ggcagatcag
    26881 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gcgtcgatgc gcttaatgta
    26941 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    27001 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    27061 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    27121 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    27181 aggaaatcgc gggaatcgta ggacggataa ggcgtttacg ccgcatccgg caatccgtgc
    27241 aaatgccaga taagacactg acgcgtctta tctggcctac aggtgattag tctttatctg
    27301 ccggacttaa cgtcacagaa gagagataat tcagcagagc aatatcgttc tcgacaccca
    27361 gcttcatcat cgcagatttc ttctggctac tgatggtttt gatactgcgg ttcagctttt
    27421 tagcgatctc ggtcaccagg aaaccttccg caaacaggcg tagaacttca ctctcttttg
    27481 gcgagagacg cttgtcaccg taaccaccag cactgatttt ttccaacagg cgagaaacgc
    27541 tttccggggt aaatttcttc cctttctgca gcgcggcgag agctttcggc agatcggtcg
    27601 gtgcaccttg tttcagcacg atcccttcga tatccagatc caataccgca ctaagaatcg
    27661 ccgggttgtt gttcatagtc agaacaatga tcgacaggct tgggaaatgg cgcttgatgt
    27721 acttgattaa ggtaatgcca tcgccgtact tatcgccagg catggagaga tcggtaatca
    27781 acacatgcgc atccagtttc ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc
    27841 cgacaacatt cacccactca atttgctcaa gtgatttgcg aataccgaac aagactatcg
    27901 gatggtcatc ggcaataatt acgttcatat tgttcatgta cttgcaccta tgtattaact
    27961 atttgatttt aaaaatatat tctatgcttt gtttagtcgt tggggcacct gtgggacata
    28021 agtacatagc tttctgttta gtacttctac ctgggactgg ttattgtctt tcatccatgc
    28081 accgtaaaca ttgtaaacca tctgggcatt cgcatgtccc atctggttgg ctatgaagtt
    28141 tggatttgct ccggcagtta aagcccagca agcataagta tgccgagact gatacgcttt
    28201 tctatggcga atttttgctc gttttaatgc agattcccat gttctgttga tggatgttac
    28261 ggcataatga actcctgagt tacctgtgcg atctaatatc tgaggattga aaacaaagct
    28321 gcatgagtga gtgcttgtcc ggccatactc tcgcagcttt acttctatct gatattgttt
    28381 accaagcctg gttaatgtcg cctgattttt taatacgtca attgctggct gtatgagttg
    28441 tattaccctg tttgttcctg catctgtttt gggtagtgta aattcctttg tctgagtgta
    28501 attccttttg acggtcaagg tacccgcttc cagatcgata tcctcccatg ccagcccaca
    28561 cagttcgcca tgccgcatcc cggtatatac agccagcgac cagaaatttt tagtttgctg
    28621 gtggtggcac gcatcaatca gtctggcaaa ctcatctttt gttagtggat cgggaggtgt
    28681 tttcgctctt ttcagtgatg gaagctcact gaaagggttt ttgcctatat acccattgtt
    28741 tgccgcaaaa cggaacatcc cggagataat tgtcatatag ctatttacag taggtacact
    28801 acgaccttta ataggcttcc tctgattttt tcctggcaac tgatatcccg ttaataattc
    28861 tttacgaaaa atgagaacat cttcctgtgt aattgatgat gccagtttgt tccctccaag
    28921 gcagtcaagg caatttcgga tagctgattt gtatctgacc atggcatttg ctgttatttc
    28981 cattttctta agctcaagcc atttttccga aagtgcctta atggttatct ctttttttcc
    29041 cagaccaaaa tgtttcaggt taggggaatt agggaactgc gcggcgtagt cgaagctccc
    29101 cattcttatt gcaaaacaaa cggaagtgcg aagttcacct gcgatcttcc ggtttttggc
    29161 ggtgtcagga acaccgaggt tttctctgac acgtttgccg ttatagtgaa accatatgcg
    29221 gagtgatccg ccatggtttt caacgcctgt cgggtatgat gcgttactca ttaaacctcc
    29281 cagacgtcca ggagcattaa caggttaacc ggaacttgca tttttggcac ctggttgttt
    29341 ctggttttcg atccagcgca taatttcctc aatgttgtat acacattcac tgaaaggccc
    29401 gggctctcct tctgctgcgt aatgacggta ttctttcccc agtaaccagg atttttttct
    29461 tgctctggca atggttcctg atttcagtcc agttgcagca ataagggttc tttctgtgca
    29521 ccatttgctg ggcgttatct gatagatgat tgtttccatt cctgactcac actatcttca
    29581 ggccacggca gtggcaccac acgtcaaaca ttcgctttac aacttcacgg cagtagaagc
    29641 cgtgagcatc tctggttaaa tcgtatcggt acccgtatct gagtcgtatc catatttcaa
    29701 actcgcgatt cacgacttat tctcctgctg tctttatgcc agttcattcg gttgcgctgg
    29761 ggccctggcc ttccgtggcc tgtatttgcg gcgttttctg gcttcaatgc gtatctttgt
    29821 caccctgact ggctttggtt cgtgccggaa cactttgttt ctgatattac gccgtgtatt
    29881 catcatccag ataaaacgct gggtatggtc gcatccgtca tcaacggtaa ttactgtgcg
    29941 gaccagttca tcgttaatat caaccatttc acccattcac tgagccttca tacgcgaatt
    30001 tcgtggcttc catcagttgc gtccataccc attcaagaaa ttgtgcgttt tctcctgagc
    30061 ggtcatattc atcgacgctg cggggcttga tggtcgccag tagtggcaca atccggtact
    30121 tcatctgttc ggttaacttg tccggtacaa gtcgccagcc gttatatttc gatggaacat
    30181 ggtggtattc agccaggaag cgtcgtatat cgtttttcat ttcctcgcat gagttctgat
    30241 catgaagtgc agcggaaagc cagcgtccga tatattcagg gattcgcaca tcaagtactt
    30301 tttctgccgt cgtcagcatt gcctgtcggc aggcgttcca gccgttgaca tagtgacgtg
    30361 ttgcatccca tgaaacctgc acatcagggt aagcatcctg ttctgttctc actggcggta
    30421 tctgaggaac agtgaatagt ggactgacga tatatttatc cagagagcct ccccgctctt
    30481 ctgcaagact ttcccacgat gtcattgtgg ggaccctgta acctgctttt ttgtgcgtat
    30541 acatccatgc cactctgccg ttatcatctg cggcggcaat gcgctgccag gcaggataaa
    30601 gcggggtgta tgtttctctg tcattacggg cgtcaggcat ggcatcaata ctgcaaactt
    30661 cccccgtaag gttattcagc caggccttcg ggctaatctc tgacgttacg atttcatcca
    30721 gttttttctc cagattttct atgtgactga ttagcgcaag gataactggc ggagtggcaa
    30781 gggagtgaaa tgcttccaga tcacatccct tgtcgccata tacggtattt tgtgcggcgt
    30841 cacgcagtgc tttcagatca gcttctttca tcgttcagac tcccttgttg ctgttttgta
    30901 ggcgcgcagg atgtcgcgtg tttttcctga taatgacgac ttaaccagga aaaagccgct
    30961 ccggctttct gtgatgtccg gcgtgcacag aagtaccgca tcaacgacac gattatgttt
    31021 tcgcaatgtc aggcgggaac tggtgataat cagttttgcc tgttcgccgt aatcggtgaa
    31081 tgaaataatc atctgatggc gatctccgtt atgaaagtgt tcctgctggt tcaatgctga
    31141 taatttctgc tgcaataagc atacctgcca gccatgcttc gccgagcatg tcctcatccg
    31201 tacatgcaag atcaccgatc tgtattgtgg ccataatgtt agtttcaccg gtatattcat
    31261 cctgtgcttc ttcatacggc aggtcttcat aaaggttttc aattgcgcag gttattacat
    31321 ccagtcccgt cagattgccg acaacgtcca cttcaaatgt ttcgcggtac tcccattgcc
    31381 cgagtgtcag acgaaccgtc tgtcttgcca tgcgcccgca taatgtcatg ttcgggtcgt
    31441 agttcatgat tgccgcagta gtgctattca tgttctgcct ccggtgtgta aatagctttg
    31501 tcgtgtttaa attcaccgtt ccagtttttc ttcataggga gttcaccgtt catgtaaaac
    31561 tggtataaac ggtggcatcc tttttccagc aatacggggg tgaatttcgt gaatgttgct
    31621 ttcccgtgtg gagtgatctg tactgcttct tcggtcaggt atctgtcacg ggcataagag
    31681 gccgtgcgcc agcggggtgt tttctccgga tcgcgttgtt cgttgaatac ccattttcgg
    31741 ccagacagcc accacattat ctggctggta tttacgccgt tcagcgcacg gcagaactgg
    31801 ggaacagtta tgcctttaga gaaatgtttt tccagactgt ctacggttgc agagagcgtt
    31861 ttattttcca gggccatagc ttcagcccgt tcttcggcct caataaccat caacgcgagt
    31921 tccttgcgag tgagtacgcg ctgaacatcg atgttttccc gctgagtgaa gtagaactcc
    31981 acaaggtctt cgtgatagtt ccacgcctga tccgtttcca gcaattttgc gtggttggct
    32041 gcgccgcgtt cggtccagag gatgagggat cgaacattgc tggcaatttt cacagagtag
    32101 ctttgagata ctctgtgctt aagttcacgt agttcatcgc cttccagcaa aaaataatgc
    32161 tttccaacaa cgaatcgttc ttcgtttcgg gaatgattct ggcgaatacg gattggtgtt
    32221 gctccataac ctgccgccag ttgttcagtg gtcacaacgc gttgaccgcg atattcgata
    32281 atttgcaaat cacgggctgc caccggcaca agttcagttg attttttcat cattacacct
    32341 cacgaaaagc tgattagtgg aacactctgg tttccggttc tgagttgtca gtggcttcac
    32401 tgataagccc atccagaatc atgtgcatcg ctctgtaacc ttccattgtg atgccggttt
    32461 tttcgccgtc gcccagagcg ccggtgaatg ctttgtacat aagaatgctt ttgagcagcc
    32521 ctgattccgc tccgtaagtt tctatgagtg cccattccat atagttctgc atagcgagac
    32581 gtaatggttt caggtagatt gtcatgccac cgttgtggcc tctgtacatg gtgcccttgt
    32641 ctgtaccgcc attctcgtta agcacttcgg tccagccgtt tctggtgagc tgttcagaga
    32701 agaacatggc ggtataaagc cagcgccagt acatgattgt cttttccggc gcgagctcac
    32761 ctgtcatgcc tttttcttct gcttcactga tgcaacacac tatttccaga ccatgccaga
    32821 tcggctcgtc aaaacagcct tcatcgagca tttccagtgc tttgttgata tcaagcagtc
    32881 cgtaaggtgt gtggatacct tcagcggtaa gaaaaaataa ctgttcagtt gcagtcatgg
    32941 tttgctctct tcttaaatgg tctttgcgcg acaagccggc aatactgatc aatccaggcg
    33001 ttgtattttt ccatccaggc atcaaactgt tttctgcgtg caaggatgcg caacaggcga
    33061 tgaaaacaac gcccgtgagc tctgtagtat tcgggcgtaa tttctcccgg atgaaagact
    33121 tctccatatt ccggatgcat caggcactgg tcaggcagac gtttttcaaa acctgaactt
    33181 tcgaatacct tcgttgtcat aaaatgagcg agatgtatga tggccgtttt tcgcgtgaag
    33241 catttcttaa cttgtccgtg gcggcagact ctgtaaacag ggccgcaatc gagttcgtat
    33301 ttatgaaaag cacggtcgat tccggcatta tttattgcac tgtattccgc atcactgaag
    33361 aaaggtggaa atacgatgta tccttccatt gtcagcgcca gaacgcgcgg atcggtttct
    33421 gtcagccatt cgttttctgt gaattcttca gaattaattt caatgatttc aggttcttct
    33481 ttcatggtta ctccgtgttc atccggtgtt aattactttt attcactctg agcgcagcca
    33541 gaagatgtgg ctcagcaatt cgtttgttgc gatatatctg tgatacagcc tggccttttc
    33601 aggttgtaaa aatccccgac aataaagctg taaattaatt actgttctta aataatgtta
    33661 ttcgttttca gtccggcaca gatgcttttc tgctttgtct gctgtacagg ataatgagcg
    33721 gaaaagacaa ttcatcatgt ttagcgcatc tggtgaacca atatcaccgc tgtcaataat
    33781 cacctgtaac aaagacaggc tttctgttat ctcgcagtgg acattatcga gtacttcaac
    33841 gcaattattc atttttcatt atctccgtta ttccggggga ggagctatgc tgttaatgat
    33901 taacgcatct ggtaatatgc ccctctttaa tgtaatcgat gcattcattc aaaagttcat
    33961 caatgattaa ctttcctgat tcagtcagat actcaccatg ttggttgata ccgacagcat
    34021 cctggtatgc agtgcgtatt gctgtttcac catctttctg cccaaactca ccacgggtaa
    34081 cgccttcaaa tcgtaatagc aactggctta tgaactgttc tgttatttca atagttgtta
    34141 actctccatc atgacgcttt acagtgagga gattacttcc tgtttttcgt ttcatccgcc
    34201 gcagtgctgc aaccgttatg cggcgacgat atgcagaaat gtattcatct ttcatttgtt
    34261 atttcccgta tgcctttttg agaaacaata ctgcaactga ccagtatccg gcatcagcca
    34321 ttaataaagc ggttttatag gcatgtttgt ctttcatgtg tcatccccat tttaaaatag
    34381 tgagaactcc cgaccgcaga ccgtcatgtt tttatgtatg attgtctgtt taatttcctt
    34441 tattcgttct cagagcatca acatactcgt aagccttttc acaggtttta ttcatggaac
    34501 gaatcataca aagtaagaaa tcatctgtct ctccgttatc accagagttt ttaaatataa
    34561 actcaagcat tgacgtgttt tcttttattt ctgctgccac ttcctcaagc ataatcaggg
    34621 gagttttcat gttctttgtt ccttaaatgc atcgcatgcg cttctggcgt attgttgtgc
    34681 cagtaaaaat atgtcatccg aaagttcatc acattcttca tcaccggaag ccgaaatgat
    34741 taaccccgct tcaagcagta ctgcaatgtg atgaaaagct gtttccggtt cgttggtgag
    34801 gcctttgaac attttcatct tacgattcct ttgtcgtgtc agaatggaaa cttgtaagta
    34861 accacatcgg atcgcagtca agtatatttg ccagaggaat cacctggctt gccagaggtt
    34921 cagccttgcc gttctcccac tggatgatgg tttcttcatc aactccaagc agcgtggcga
    34981 gttcagcagt ggatagaccg cagctttcgc gttgggtgcg gatgccatca cggcaatctt
    35041 ttttgtttct cggaggtaac agaatatgtt ttagttgttg gattttttcg attccaacct
    35101 caaggagtac gtgacggttt ctttccgata cagactccag actttcatga agcagcgatt
    35161 ctacttgttc gataagcatc aattgtgaag tagacataaa aaaacctcgt aaccaatatt
    35221 tcgaaatcta ggttacgagt gagtaatgta ggtgtcaaga gcaattaatg ttttttgttg
    35281 aattaaattt gcatacgcat ctcaacgaca acaccaataa tttcacaatc cccattgatg
    35341 gggatcagtg gatagcgtgg gtttagtggc tttaggaaac attgcccagc gtcttctatg
    35401 tactgtttaa gggttgcctc tccggagttt ttgagcctgg cgacgacata ttttcctgaa
    35461 aaaacttctt tgtcagggtt aacaagaatg ttcatgcctt caaaaataga gaactctcca
    35521 ttctgtgatg tcattgagtc ccctttcact tccagccaga agccgtgatc gcccgcatat
    35581 atatcgctac caatccattc ttctggataa gctatgtaat cttcgcagtt ccaggggcca
    35641 gcactcaccc atgttagttt cggatatcta taacggtttg gagaaactgg cttaggattt
    35701 cggacgtttt gaatgctatc tgtaggtgat ggggcgctgc cgtcaagaag ccatttagga
    35761 gagcatttca atgcatccgc gattctaaat aggtttgaag atcttgtgtc ttgtgagtct
    35821 cctaactcta ttttgctgat tgttacacgc gagacaccgg tcaattttgc caggtcatct
    35881 tgtgacattc ctagctgtac tctgcgcatt aacagtctgc caccaaagct atcgtgatcc
    35941 atcttctccc ccttcttgtc gaaataatcg tttcgaaaca aatatagcaa aaaatcttga
    36001 aatctaggtt tcggaaaatt aagattaatc gaaacaaacg tgtcgaggtg gatatggatt
    36061 tgtatgaaat tctaaaaaac atgtttggat cgaatgttga aataggtcgt tattttcccc
    36121 gaaggggaag ggctagaacc ggacaggctg taggtaagtg gaaaacccga ggtgtgccgg
    36181 aagacgtggc gatcctttgt catctggatc ccaaaattcc ttatcagcat ccgtcgctca
    36241 tgaatgccag tcatgaatcg tgatgttctg ccggaggctg tatgtcccct gattacgttc
    36301 agatggagat gcctgccagg tacagccagg cagattcaga gtggattaag cggcagttac
    36361 agagcctgcc ttcctcactg agacagaagg tcgccctgaa atacgcggag gtttacgaag
    36421 ttacttttga ttccgagcct gtttcattcc gtaaggagaa cagagcgagg catgaagcca
    36481 atgtacggct gcgcagattc gttgaaacac acgggcgtgc attacagggg tatacgactc
    36541 agccgcccct ggcaggaacg caacagcgta cctgaatggt gttggtctta aaggtgaccg
    36601 acgggctgag accccacttc tcaggatgca cttatgtact accaagctag tac
//
LOCUS       DAZGAP010000020        36343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGAP010000020 DAZGAP010000000
VERSION     DAZGAP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.020"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            242..832
                     /locus_tag="ACHLAM_000886"
     CDS             242..832
                     /locus_tag="ACHLAM_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7852456.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            941..1681
                     /gene="yadV"
                     /locus_tag="ACHLAM_000887"
     CDS             941..1681
                     /gene="yadV"
                     /locus_tag="ACHLAM_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO7852457.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1716..4316
                     /gene="htrE"
                     /locus_tag="ACHLAM_000888"
     CDS             1716..4316
                     /gene="htrE"
                     /locus_tag="ACHLAM_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO7852458.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            4333..4905
                     /locus_tag="ACHLAM_000889"
     CDS             4333..4905
                     /locus_tag="ACHLAM_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7852459.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            4917..5525
                     /gene="yadL"
                     /locus_tag="ACHLAM_000890"
     CDS             4917..5525
                     /gene="yadL"
                     /locus_tag="ACHLAM_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO7852460.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            5552..6148
                     /locus_tag="ACHLAM_000891"
     CDS             5552..6148
                     /locus_tag="ACHLAM_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO7852461.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            6174..7463
                     /locus_tag="ACHLAM_000892"
     CDS             6174..7463
                     /locus_tag="ACHLAM_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO7852462.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            7575..8369
                     /gene="panB"
                     /locus_tag="ACHLAM_000893"
     CDS             7575..8369
                     /gene="panB"
                     /locus_tag="ACHLAM_000893"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO7852463.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8381..9232
                     /gene="panC"
                     /locus_tag="ACHLAM_000894"
     CDS             8381..9232
                     /gene="panC"
                     /locus_tag="ACHLAM_000894"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO7852464.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(9314..9529)
                     /locus_tag="ACHLAM_000895"
     CDS             complement(9314..9529)
                     /locus_tag="ACHLAM_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7852465.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGFQKGYKEGLQKVRLAQRLL
                     SKGMSREDVAEVTALSLAEIDKLINSN"
     gene            complement(9598..10566)
                     /locus_tag="ACHLAM_000896"
     CDS             complement(9598..10566)
                     /locus_tag="ACHLAM_000896"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7852466.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVRQEV
                     RQEVIQEVRQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            10840..11220
                     /gene="panD"
                     /locus_tag="ACHLAM_000897"
     CDS             10840..11220
                     /gene="panD"
                     /locus_tag="ACHLAM_000897"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO7852467.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(11224..12453)
                     /gene="yadE"
                     /locus_tag="ACHLAM_000898"
     CDS             complement(11224..12453)
                     /gene="yadE"
                     /locus_tag="ACHLAM_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7852468.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12517..12957)
                     /gene="yadI"
                     /locus_tag="ACHLAM_000899"
     CDS             complement(12517..12957)
                     /gene="yadI"
                     /locus_tag="ACHLAM_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7852469.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(13062..13832)
                     /gene="yadH"
                     /locus_tag="ACHLAM_000900"
     CDS             complement(13062..13832)
                     /gene="yadH"
                     /locus_tag="ACHLAM_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7852470.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13829..14755)
                     /gene="yadG"
                     /locus_tag="ACHLAM_000901"
     CDS             complement(13829..14755)
                     /gene="yadG"
                     /locus_tag="ACHLAM_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7852471.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14864..15526
                     /gene="can"
                     /locus_tag="ACHLAM_000902"
     CDS             14864..15526
                     /gene="can"
                     /locus_tag="ACHLAM_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO7852472.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15567..16103)
                     /gene="hpt"
                     /locus_tag="ACHLAM_000903"
     CDS             complement(15567..16103)
                     /gene="hpt"
                     /locus_tag="ACHLAM_000903"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO7852473.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            16309..18699
                     /gene="gcd"
                     /locus_tag="ACHLAM_000904"
     CDS             16309..18699
                     /gene="gcd"
                     /locus_tag="ACHLAM_000904"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO7852474.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18895..20445)
                     /gene="cueO"
                     /locus_tag="ACHLAM_000905"
     CDS             complement(18895..20445)
                     /gene="cueO"
                     /locus_tag="ACHLAM_000905"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO7852475.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20611..20958
                     /gene="yacC"
                     /locus_tag="ACHLAM_000906"
     CDS             20611..20958
                     /gene="yacC"
                     /locus_tag="ACHLAM_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO7852476.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            21064..21930
                     /gene="speE"
                     /locus_tag="ACHLAM_000907"
     CDS             21064..21930
                     /gene="speE"
                     /locus_tag="ACHLAM_000907"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO7852477.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21946..22740
                     /gene="speD"
                     /locus_tag="ACHLAM_000908"
     CDS             21946..22740
                     /gene="speD"
                     /locus_tag="ACHLAM_000908"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO7852478.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(22778..23140)
                     /gene="yacL"
                     /locus_tag="ACHLAM_000909"
     CDS             complement(22778..23140)
                     /gene="yacL"
                     /locus_tag="ACHLAM_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO7852479.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(23315..25912)
                     /gene="acnB"
                     /locus_tag="ACHLAM_000910"
     CDS             complement(23315..25912)
                     /gene="acnB"
                     /locus_tag="ACHLAM_000910"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO7852480.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            26267..28024
                     /gene="yacH"
                     /locus_tag="ACHLAM_000911"
     CDS             26267..28024
                     /gene="yacH"
                     /locus_tag="ACHLAM_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO7852481.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSDHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(28095..29519)
                     /gene="lpdA"
                     /locus_tag="ACHLAM_000912"
     CDS             complement(28095..29519)
                     /gene="lpdA"
                     /locus_tag="ACHLAM_000912"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO7852482.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29844..31736)
                     /gene="aceF"
                     /locus_tag="ACHLAM_000913"
     CDS             complement(29844..31736)
                     /gene="aceF"
                     /locus_tag="ACHLAM_000913"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO7852483.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31751..34414)
                     /gene="aceE"
                     /locus_tag="ACHLAM_000914"
     CDS             complement(31751..34414)
                     /gene="aceE"
                     /locus_tag="ACHLAM_000914"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO7852484.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34575..35339)
                     /gene="pdhR"
                     /locus_tag="ACHLAM_000915"
     CDS             complement(34575..35339)
                     /gene="pdhR"
                     /locus_tag="ACHLAM_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO7852485.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(35795..36085)
                     /locus_tag="ACHLAM_000916"
     CDS             complement(35795..36085)
                     /locus_tag="ACHLAM_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO7852486.1"
                     /translation="MYNFKDKVEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDKPENILKIVKEWRRSQGLSLFKDSE"
     gene            complement(36086..>36343)
                     /locus_tag="ACHLAM_000917"
     CDS             complement(36086..>36343)
                     /locus_tag="ACHLAM_000917"
                     /inference="COORDINATES: protein motif:HMM:NF044473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852487.1"
                     /translation="DTLWLEVSQYPELIAQFSSGNQTRIKQGLIAKAPLEGWHYGPKE
                     IVKKFHMYHCVAIEYSGEIYDIDNLRRAHLICMMKFITGDS"
BASE COUNT         9203 a         9453 c         8622 g         9065 t
ORIGIN      
        1 atacaccatc cctcttagct ttcaaattta attttactaa cttttatttt tagtaaaatt
       61 aaattgcata tgctatcaca ttatgcaaaa tagccatccc gatgatgaat gaatgtaata
      121 gttggtaaac aacagacaaa catcattaac acagtaacga ttttgttacg cagcattgca
      181 gcagactctt atcgtcatgc ggtgcggtta tctctctatg gaatattatt caaggatttt
      241 tatgtctaaa aaattaggtt ttgcgctgtc tggtattatg ttggcaatgg ctgctggtac
      301 tgcatttgct gctaacgata tggacggtgg tcagttaaat attagcggcc tggtcgttga
      361 taacacctgt gaagctcgtg ttgatggcgg taacaaagat ggtttgatcc tgctgcaaac
      421 cgctactgtt gctgaaatca ccgatggtgt actggatacc accgtaggtg ctaaagctaa
      481 gccattcagc attactattg actgcagcaa agctaacccg gctccgggta ctactgcaaa
      541 aatgactttc ggttccgtgt tctttagcaa tagcaaaggt actctgaata acgatatgtc
      601 tatcaataat ccatctgatg gtgtgaatat tgccctgcat aatattgaag gttccactat
      661 taagcaggtt caggttaata acccgggtga tgtttacagc aaaactctgg atgcaacgtc
      721 taaaactgca acctacgatt ttaaagcatc ctatgttcgt gctgatgcaa gcaaagcagc
      781 aactgccggt tatgtaaaaa ctaactcagc atataccatt acttaccagt aaatatcagc
      841 cacctaaatt gattgggtga atatatatag ggaataacat caaacatatt tatttttgaa
      901 cgttattccc tgttttaatt aactctgtga ggaacggttt atgtttttta atactaaaca
      961 taccgcagca ttgtgcttag cgagctgtat ggcttttagc tcatcagcta ttgcagacat
     1021 cgtaatttca ggtacacgcg tagtttataa aagcgaacaa aaaagcgtca atgtgcgtct
     1081 ggaaaataaa ggcaataacc cattgctggt tcagagttgg ttagatactg gagatgacaa
     1141 cgctgaacca ggcagtatta ctgtcccctt taccgctacg ccaccagtat cacgtattga
     1201 tgctaaacgt ggccaaacaa tcaaactgat gtacaccgcc agtagtgctc tgccaaaaga
     1261 cagagaaagt gtgttctggt ttaacgtact ggaagttcca ccaaaacccg atgcagcaaa
     1321 agcagcaaat caaagcctgc tacaactggc atttcgtaca cgtataaaac tgttctatcg
     1381 cccggaagga ttgagtggaa ttccatctga tgcgccattg gctctgaagt ggtcctgggc
     1441 aacatctgaa ggcaaagcgg cattacgcgt tgataaccct acgccctact atgtctcttt
     1501 tagcagtggt gatttagaag ccaacggaaa acgctatccc ctggatgtga aaatgattgc
     1561 accgttcagt aacgacgtca tcaaggtaaa tgggatgagt ggcagaacaa gctctgcaaa
     1621 agtgcatttt ttcgccatta atgattttgg cggctctatt gaaggcaacg ccaaccttta
     1681 atctggcaag gatataccac agcaggaagc agaacgtgac tattaaatcc actaaaaaat
     1741 atcataatca ccttacccgc atcgccacgt tttgcgcgct gctgtatagc aacagtgctt
     1801 tatgcgctga actcgtcgaa tatgaccata catttctgat gggaaaggat gcttcaaata
     1861 tcgaccttag tcggtataca gaaggcaacc cgacactacc aggcatttac gatgtcagtg
     1921 tgtatgttaa cgatcaacct atcatgagcc aaagcattgc ttttactgtt attgaaggta
     1981 aaaagaacgc tcaggcctgc attacgcaga aaaatttatt gcaatttcat atcagttcac
     2041 cagacaagaa tagcgaaaaa gccattttgc ttaagcgaga cgaagatctt ggtgattgcc
     2101 ttaatctggc tgaaatgatc cctcaatcat ccattcgtta cgatgttaac gatcaacgcc
     2161 tggatatcga cgtcccccag gcatggatta tgaagaatta tcaaaactat gttgatccct
     2221 ctttgtggga aaatggcatt aatgctgcca tgctttccta caacctcaac ggatatcaca
     2281 gtgaaagtcc aggcagaaca aatgacagta tctacgcggc attcaacggt gggataaact
     2341 taggtgcctg gcggttacgt gcctcgggta actacaactg gatgaccaat gtccatagcg
     2401 attacgattt tcagaatcgc tatttacaac gcgatcttgc gtcactacgt tcacaactgg
     2461 ttattggtga atcttacaca accggcgaaa ccttcgattc agtcagtatt cgcggtattc
     2521 gcttatacag tgacagtcga atgctgcctc cggtattagc cagttttgcg ccaatcattc
     2581 atggcgtagc gaataccaat gccaaagtaa ccgttatgca aaatggttat aaaatctatg
     2641 aaacgacggt gccgccaggg gcctttgcca ttgatgatct tagcccgtca ggttatggca
     2701 gtgatctcat tgtgactatc gaagaagctg atggcacaaa acggacgttc tcccagccat
     2761 tttcatctgt tgtgcaaatg ctgcgccctg gcgttggtcg ctgggatatt agcgccggtc
     2821 aggttttaaa agacagtatc caggatgagc ctaatttatt tcaagccagc tactactatg
     2881 gtttgaataa ctacctgaca ggatataccg gtattcagct aactgataat aactataccg
     2941 ccggattgct ggggcttggt atgaataccc ccgtgggggc tttttctgtc gatgttactc
     3001 actcaaatgt cagcattccg gacgataaaa cctatcaagg acaaagctac cgtatttcct
     3061 ggaacaagtt atttgaaaat accagtacat cattaaatat cgcggcctat cgctactcca
     3121 cccagcatta tctgggcctc aatgatgcgc tgacgctgat tgatgaagta gaacatcctg
     3181 agcaagatct tgagcctaaa tccatgcgca attactcgcg catgaaaaat caggtcacgg
     3241 tcagtatcaa ccaaccgctg aaatttgaga aaaaggatta cggttcattt tatctctcgg
     3301 gaagctggtc agattactgg gcctccgggc aaaacagtac taattactct attggttata
     3361 gcaatagtgc gtcctggggc agttacagta tcagtgctca acgttcgttg aatgaagatg
     3421 gtcaaaccga cgatagcatt tatctcagct tcacgattcc aatagaaaat ttactgggta
     3481 cggaacaccg cagctccggc ttccagagta ttgatacaca attaaacagc gactttaagg
     3541 gtaataacca actcaatatc agcagtagtg gttacagcga taccaatcgc atcagctaca
     3601 gcgtaaacac gggttatatg atgaataaat ccagtgatga cctgagttat atcggcggtt
     3661 atgccagtta tgaatcgccg tggggaactc tgtcaggttc agcctcagca agcagtgata
     3721 atagtcgcca attctcactc aatactgatg gtggatttgt gttacatagc ggcgggctga
     3781 cgttcagtaa tgacagtttt agtgattccg acactctggc tgtcattcag gcgccaggag
     3841 caaaaggcgc tcgtattaac tacggaaaca gcaccgtaga ccgctggggt tatggtgtga
     3901 ccagcgccct ttcgccatac catgaaaacc gtatcgcact ggacattaac gatctggaaa
     3961 acgatgttga attgaaaagc accagtactg tcgccgtccc gcgccaggga gccgtcgtct
     4021 ttgctgattt tgaaacagtt cagggacaat cggcaattat gaatatcgtg cgaagcgatg
     4081 gtaaaaatat tccatttgca gcggatattt atgatgaaca aaacaatatc atcggcaacg
     4141 taggtcaggg tggtcaggcc tttgttcgcg gtatcgggca ggaaggaaat atacgaatta
     4201 cctggattga agagggcaaa ccagtcagtt gttttgctca ttaccaacaa aacacgacat
     4261 cagaaaaaat agcgcaatct attattctga acggacttcg ttgtcagata cagtaaccac
     4321 aagggacttc caatgataaa aaccacacca cataaaatag caatactcat ggggctatta
     4381 ttatcgccat ctgtatttgc aacggatgtt aatgtcgatt ttaccgctac cgtgaaagcg
     4441 acaacatgta acatcaccct gacatcgctg aatggttcaa acgtcgttga taatggcaat
     4501 gatagttata ccctgataat ccccaagatg ggactggata agatagtcaa taaagcgtca
     4561 caatctgagg ccaacttcaa attagtcgcc agtgggtgta gcagtggaat tagctggatt
     4621 gataccacaa tgactggaaa tcagtctggt agtacatcgc tgattattcc acaatcttct
     4681 gactcctcct caacaaccag taatattggt atgggattta aaagattaga gacttcgggc
     4741 gatacgtttc tgaaacccaa tagcgcggaa tacatccgct ggaaaaccac agaaattagc
     4801 accagtggtc tggaaatgac tgtcgcactg cgcgaaacaa ccccaggaca gggcataccc
     4861 gggaaatttc gcgcacaggc aacgttcaat ttcatctatc aataacaagg atgacaatga
     4921 tgatattaaa aaaattatgt tatggattat cgggtagtct atttctggct tcatcactgt
     4981 taaccatttc ctcacatgcg gccactgata gtattggcct gactgttact acagacatag
     5041 aaatgggtac ctgtacatca acgttgactg atgacggtac agctaaactg tccaccatca
     5101 atttcggtga tgtatatatt tctgaaatta atgccgaaac taaaattaaa accttcaaat
     5161 tgcagttcaa agactgtgcg gggattcccg ggaaaaaggc caaaataaag ttgaccccac
     5221 gagccttatg cgaaggtaat tctaataacg gccctggatt tgctaacgca tcaacggcaa
     5281 cagcaaaagc agcggcagtc gccgttgagg tctggagtac gtccacacca ggtaaaaacg
     5341 gcgcaaaaca attcagttgc gtcacccccg caacagaaga agtcagtatt gctggtgcga
     5401 cgggaggtga tgttgtcgat tacccgatga gtgctgtttt ggtggtcgcg aaagataaaa
     5461 ccgttacaga tgtcaccgcg ggtgatttca ccgctccggc aacatttact gtgacttata
     5521 actaacaggc agaccgattc aggaactaaa tatgcgcccc actcaacgta atctgatgaa
     5581 aaaatcaatt ctgcttctgt ctttgctatt cagcaccact ttccctgtgg ttgcaggaca
     5641 ggatgttgac attgttgcaa atataaaaaa taacacctgc caaagtggaa tcagcaacaa
     5701 cggaaatatt gatttaggtg tcgttggcgt gggcgatttt accggaaaca tttcagccga
     5761 aaattatcac cccggtggaa aagagttcac tattacagta caggactgca cacttcaggg
     5821 cacagggaat gtgttaaatc agttacatat tgattttcgc gcactcagtg gcgtaatggc
     5881 gacaggttcc agtcaaatat tcgccaatga agacaacacc ggggctaaaa atgttggggt
     5941 agttatattc tctattcagg acccggcaaa taccttcaac gttcttggcg taactggtag
     6001 ctcacgttcc gtttatccgg tcatgtcatc agcactaaac aattcatcct ggaaatttag
     6061 cactcgaatg caaaaaatcg atccagcgct gaatgtcacc tctggccaac ttataagcca
     6121 tgtgttagtg gatatttact acgaataaca gacaaagtgc tgcaagcatt atcttgcagc
     6181 atgacaatgg agtaaaaatg aagatattct tcagatactt tcttttttta gcactatgct
     6241 cttgttgtta tacagcaagt gccggcaccg atgataacgt cagctatctt gttgggaatg
     6301 attacggagt gggtccggga gaccaaaagt ggagagaaac aggccccagt gcagacatta
     6361 ctgtcaaatt caggtattca acaagcacaa ataacttagt tttttataaa ccaacccaac
     6421 tcggaccaac aggtgtgtca ttatactggt ctcagttaaa tagcgcttca ggtggaggtt
     6481 acctttactg caacagaagt aacactgcaa gcggtgagcc tatggcaatt gaaaatcaaa
     6541 tggttgattc gggtaaacgt tatggcgggc ataaactatt tgaaacatct gtcccaggcc
     6601 tgtattatac attaaagata tcaaacattt ggtcatcata caccacaacc aatatcagtt
     6661 catcaggtat gtatatcggc gattctccaa ctcaatattt tcggtggatc cccgaaagtg
     6721 aagccactct atattggtct tgtaacaatg caaactcatc taataaatac tgggccgtag
     6781 gtggagtttg gcagtcgctc acggtggaat tttataccga taccaacttt gaccccacaa
     6841 agacaaacaa ggtaacatta tctaagccgg gtggttatct ctactcattc aaaggatatg
     6901 gtcttggtgg cggtatcaac gatcacagct atttccttaa aatagatttt gacctggata
     6961 atatgacgtt atccaaccca acgtgtttta ctgctgcact tactggccca tctgtaagcg
     7021 gttcaacagt taaaatggga gattataccc cagcgcaaat aaaaaatggc gcaacagcgg
     7081 ttccttttga tatcacgcta cagaactgta ttcgtgttcg caatatagaa acaaaactta
     7141 tgtcaaacaa ggtgggcagt gtaagcaaag aacttctggc taacacactc acaggaaata
     7201 acgcagcaaa aggtgttggt gtactgattg aaggtttaaa aaacactaaa agtgcacaaa
     7261 tggtactgaa accaaatgat tcaacgtctg tttataaaga ttacgaaaca gaagatgaca
     7321 ctaccggagg aatttacccg gataaaggaa acggtacctc acaacctctt catttccagg
     7381 ctacattaaa acaagacggt aatattgcta tagaaccggg ggattttaaa gccaccagca
     7441 cttttcaggt tacctacccc tgataacact attgcaccgc cagacaatgg cggtgcgata
     7501 cggataaatc attgccccca ccaatgtgac tgataaaatg cgagacagca caacatcatt
     7561 tatcaggata cgctatgaaa ccaaccacga ttgcttcatt gcagaaatgc aagcaggaca
     7621 aaaaacgttt cgcaaccatc accgcttacg actatagctt cgcaaaattg tttgccgaag
     7681 aaggacttaa cgtcatgctg gtaggcgatt cactgggcat gacggttcag gggcatgact
     7741 ccacgctacc ggttaccgtt gccgatatcg cctatcacac cgccgctgtg cgtcgcggcg
     7801 cgccaaactg ccttctgctg gctgacctgc catttatggc gtatgccacg ccggaacaag
     7861 cctttgaaaa cgccgcaacg gtaatgcgtg ccggagccaa catggtcaaa attgaaggcg
     7921 gtgagtggct ggtggaaacc gtacaaatgc tgaccgaacg tgccgttcca gtttgtggtc
     7981 atttaggcct aaccccacag tcagtgaata ttttcggtgg ctacaaagtc caggggcgcg
     8041 gcgatgaagc gggcgatcaa ctgctcagcg atgcattggc attagaagct gctggggcac
     8101 agctgctggt gctggaatgc gtgccggttg aactggcaaa acgtattacc gaagcactgg
     8161 cgatcccggt tattggcatt ggcgcaggca acgtcactga cgggcagatc ctcgtgatgc
     8221 acgacgcgtt cggcattacc ggcggccaca ttcctaaatt cgctaaaaat ttcctcgccg
     8281 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     8341 acccgggcga agaacacagt ttccattaag gagtgacgtt gtgttaatta tcgaaaccct
     8401 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggtaagcgcg tggcgctggt
     8461 gcccaccatg ggtaacctgc acgatggcca tatgaagctg gtcgacgaag ccaaagcccg
     8521 cgccgatgtg gtagtggtca gtattttcgt taacccgatg cagtttgacc gcccggaaga
     8581 tctggcacgt tatccacgga ccttgcaaga ggactgcgaa aagctaaaca aacgtaaagt
     8641 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtacag aaacccacac
     8701 ctatgttgat gttcctggtc tttccaccat gctggaaggt gccagccgtc cgggacattt
     8761 tcgcggcgtt tcgacgattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     8821 cttcggtgaa aaagattttc agcaactggc gctgatccgc aaaatggttg ctgatatggg
     8881 ctttgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctgag
     8941 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcccggtc tgtacaaagt
     9001 tttaagttcg attgccgaca aattgcaggc tggcgaacgg gatctcgatg aaattattac
     9061 tattgccggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgtga
     9121 tgccgacacc ttgctggagg tctctgaaaa cagcaaacgt gcagtgattc tggtagccgc
     9181 ctggcttggc gatgcccgcc tgatcgacaa caaaatcgtc gaactggcgt aatccttatc
     9241 tggcgctacg gctgatggcg ccagttattc attgccacgt tatctgtagg caattattca
     9301 tatcgtagac aacttagtta gagttaatta gcttatcaat ctctgcaaga gataaagctg
     9361 tcacctctgc aacgtcttcc cgagacattc ctttactcag aagacgctgg gccagtctta
     9421 ccttctggag tccctcttta tatcccttct gaaatccctt ctgaaatccc ttctcttcaa
     9481 accactgcgc caacgtctgc ctggtttctc tccgtgtctt tatttccata tgttccctta
     9541 attatctggt cagttaacaa taacaattat ctggctataa ctgataatgc agataactta
     9601 gttaatcagc ttatcaacct cagcaagagg taaatttgcc atctctgcaa cgtcttcccg
     9661 agacatccct ttactcagaa aacgcagggc gtattcctgt cttacttcct gtctaacctc
     9721 ttgtattacc tcctgtctta cctcctgtct tacctcctgt cttacctcct gtcttacctc
     9781 ctgccttacc tcctgccttt cctcctgtct tcccttctct tcaaaccact gcgccagcgt
     9841 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     9901 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     9961 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
    10021 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
    10081 cggtgtgatg gtgatatcca ccagcgggaa agggttgttg taaacgcgtc gcgccagctc
    10141 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
    10201 ataaaacaga atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
    10261 catggcggca atggcatagc gcatcatgcg aaaggtcatt ttcttatccg gcttgctttg
    10321 atgttcaatc accacatgca aataaccggg attaccctgc atttgcacgg aataaagcac
    10381 gtcagtgctg tgccctttca ggctttcttc aataaaactc cccgactcaa gatgcagcgt
    10441 gtcgaggtca cacagttcgc gtagttccgc tggcaaatgg atatccagaa agtcacgagc
    10501 cgtttccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
    10561 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgcgc
    10621 cgtcgtcagc cgcctcttta ttgtttaccg agcagcgttc agagaaattt caattggtta
    10681 caggcgttac atggcacgct tcggcacggt acgcaaactg ctgatgcaaa tgccgtcaga
    10741 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
    10801 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
    10861 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
    10921 attgaccagg attttcttga cgcagctggt attctcgaaa acgaagccat tgatatctgg
    10981 aatgtgacca acggcaagcg tttctccacc tatgccatcg cggcagaacg cggttcgaga
    11041 attatttccg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
    11101 gccagcttcg ttactatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
    11161 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcctga
    11221 taatcacccc tgcggctggt tactcaccag ccgcgacatc gtctcgagcg aatccgttct
    11281 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
    11341 ggttgtcacc gccaggtgaa atccggcttc ttttgctgcc ttcacggctt tgtcattaaa
    11401 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
    11461 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagcaata tgggtcggcg
    11521 ataaccatcc acccgatgca aaaaatgggt atgtgactgg aaatcaaata catcgcgaat
    11581 ttcgttaagt tcagaaacgc tcataaattg cagcgatttt gggtcccact tctgcgggtg
    11641 acgtttgatg cgcgaggtaa caataaacgc cgtcgccttc atgccatatt gtttcaacac
    11701 aggatacgca tagcggctca ccgatttgag gccatcatca aaggtaatca ccaccgctcg
    11761 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcaccatac tcagtgtcgt
    11821 gtatcccctg tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt
    11881 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aggttagcac
    11941 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
    12001 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
    12061 caaattgtcc gccagtaccc caaacggcgc actgcccgca ctcggcgcgt tatagaccgg
    12121 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
    12181 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatga cctttatcga taaacccttt
    12241 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
    12301 cccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
    12361 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacgcttg cggtaaacag
    12421 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
    12481 cttttattgt agcaaaagcg tcatcagaat cgcgggttaa tactcataca aattatgttt
    12541 gaggacgaaa ggcggatttt tttgttgctg gtgccaaaga ctactcacct ccggcgcacc
    12601 cagttcgaca atacgctcgc gaaactctgc gctggataac gtttcacggt agaccagcat
    12661 ctgctcaatt aacggtaacg tgacgccaga aatcacttcg caacgggaat gtttatggct
    12721 taatagcgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
    12781 ttcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
    12841 taacccacgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
    12901 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
    12961 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
    13021 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
    13081 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtaca
    13141 ccgaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
    13201 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
    13261 agtgagtaaa agaccccgcc caaatacgta agtggcgtta acacaaaggt tggcaccagg
    13321 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaacaac
    13381 accgccgtga gcaccagcgt taaagcgaca aatacccacg aatgcacctg aaacggcaca
    13441 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
    13501 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
    13561 ttacgctgaa acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatg
    13621 accgacatca tgatcagccc cggtacaatg aactgcatgt agctgaagcc atgcatatcg
    13681 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgacc
    13741 ggtggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
    13801 ctttttagtg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
    13861 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
    13921 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcaga acttcaactt
    13981 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacgggcttt
    14041 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
    14101 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
    14161 cttcttccag gtagtgtgtg gtgagaatga tagtggtgcc tttgtcgttt aaatccttca
    14221 aaaagcccca cattgagcgg cgaagttcga tatccacgcc tgcggtcggt tcgtcgagaa
    14281 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
    14341 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatact
    14401 tttcgctgcg aatgtacgct tctttgcgct ccacaccgta gtaccctgcc tgattcacca
    14461 caatttgctg cacggtttca aacgggttga agttaaattc ctgtggcacc agacccaact
    14521 gacgtttagc gtttacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
    14581 aggttttatt taccagagag ctgataatac cgatagtggt cgattttccg gccccgttcg
    14641 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atcgattcca cgaagcgcct
    14701 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
    14761 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
    14821 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
    14881 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
    14941 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
    15001 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
    15061 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
    15121 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
    15181 cgcagttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
    15241 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
    15301 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
    15361 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
    15421 cttgctgcgt gatctggatg tcacagcgac taaccgcgaa acccttgagc aacgttaccg
    15481 tcacgggatc tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
    15541 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccgatatac
    15601 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
    15661 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
    15721 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
    15781 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
    15841 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
    15901 gaggcggtca taaaatcgac ttcatgagat acctgaactt cacggcacag gtccgccata
    15961 aacataaatg agccacgcag cagaccaacc agcaccatat cgctgccact gtctttgtaa
    16021 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
    16081 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
    16141 acgggcaggt ttcagcaccg caacaaagcg caagacacgc agtataccag taaaactatt
    16201 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac gatcacacat gttaaaacct
    16261 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
    16321 aataccggct cgcggcgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
    16381 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
    16441 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
    16501 ctttggctgt acgcagccct gctgctcggc actatgattt ggggcgtctg ggaagttggt
    16561 ttcgacttct gggcgctgac tccgcgcagc gacattctgg tcttcttcgg catctggctg
    16621 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
    16681 gtggtggcac tgctgattag cggtggtatc ctggcctggg ccggatttaa cgatccgcag
    16741 gaaatcagcg gcaccttaag cgccgatacc acgccagcag aagccatctc ccccgttgcc
    16801 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
    16861 caaattaacg ctgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
    16921 gtgaagcagc cgaacgatcc aggtgaaatc accaatgaag tgacgccaat taaagtgggc
    16981 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
    17041 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtccttcca gcatgtaacc
    17101 tgccgtggcg tctcttatca tgaagccaaa gcagaaactg cttcgccgga agtgatggcg
    17161 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gtctgattgc aattaacgct
    17221 gaaaacggca agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    17281 atgccggaca ccaaaccggg tctgtatgaa ccgacttcgc cgccgattat caccgataaa
    17341 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgtgaaac atctggcgtg
    17401 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc tggtgcgaaa
    17461 gatcctaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcgtgg
    17521 gcaccagcgg cctatgacgc gaagctggat ctggtgtatc tgccgatggg cgtgaccacg
    17581 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    17641 gcgctgaatg ccacaaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    17701 tgggatatgg atcttccggc acagccgacg ctggcggata tcaccgttaa tggtcagaaa
    17761 gtgccagtta tttatgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat
    17821 ggcgaactgg tggttccggc accggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    17881 tacgtaaccc caactcaacc gttttctgaa ctgagcttcc gtccgacgaa aaatttgagc
    17941 ggtgcggata tgtggggagc caccatgttt gaccagctgg tgtgccgcgt gatgttccac
    18001 cagatgcgct atgaaggcat tttcaccccg ccatctgaac agggtacgct ggtcttccct
    18061 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccgaatcg tgaagtggca
    18121 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tcctggcaac
    18181 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccgcag
    18241 tacggtgtac cgtatggcgt cacgctcaat ccgttcctct caccgtttgg tctgccgtgt
    18301 aaacagccag catggggtta tatctcggcg ctggatctga aaaccaatga agtggtgtgg
    18361 aagaaacgta ttggtacgcc gcaggacagt atgccattcc cgatgccggt acctgtgccg
    18421 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    18481 atcgccgcta cggcagataa ctacctgcgc gcttataaca tgagcaacgg tgaaaaactg
    18541 tggcaaggtc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggt
    18601 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    18661 tatattgtgg cttatgcgtt gccggatgat gtgaagtaag gcttgctcag attgctgaca
    18721 acgtgcgatt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaacattt
    18781 gcaaattcaa tacattgcgc gtaggcctga taagcgtagc gcatcaggca gttttgcgtt
    18841 tgtcatcagt ttcatgcccg gagagatccg ggcatatttc cgaatacggt ctttttatac
    18901 cgtaaaccct aacatcatcc ccgtatcttc atgctccagc aaatggcagt gcgccatata
    18961 agcatgttct ttcggtgcat cgtgattaaa cttcaccagc acttcgctga cattaccttc
    19021 taccttaacg gtatctttcc agcctgcgcg atgcgtcgct ggcggtttgc cattttctga
    19081 caagatacgg aactgcgtac cgtggatatg gaacggatgc agcatcatgt cgcccacgcc
    19141 agagataacc caacgttcgt attgcccttt cgccgccgca aacatcggct tgttcatatc
    19201 aaacgcctga ccgttgattt tattggcatg gtggaaatcg aacttcccgc cgtggttcat
    19261 atgattcata ttgccgtgcc ccatatggcc catcatctgg ctgtgatcca tcccggccat
    19321 cgcctgatcg ccatatttct ccatcagcat ctgcatcccc atcatatcga gcattgggtc
    19381 catagaaagt tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg caggcaggct
    19441 acttaatgtg tctggcaaag caccagaggc actaatggca atcggctgaa tccgcattac
    19501 cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc tggctgaccg gcagcgtcac
    19561 caggtcaaag ggtttgttgt cgttaacctc caccagcact tcaaaacgct cgcccatcag
    19621 cactggcagc tcgttcacct tcaccggttc aggtagcaga ccgccgtcgc tggcaatcac
    19681 ataaagcggg cgattgtcgc tggtggcgaa attgagcgag cgggcattac agccattgag
    19741 caaacgcagg cgcagccagc cacgcggggc agcgtgttgt gggtagatta caccgttggt
    19801 cagcaacgta tcgccaaacc aacccacagc ggcggtcatc acatccagtt gataatcaat
    19861 ctgcccgtca gcgttaaatt ttttatcctg aacaataacc gggacatcat caatacccca
    19921 ctgttttggc agcattaatt tcaggatctc gtcatcttca atcaccacca aaccagccag
    19981 ccccatcgcc acctgacgtc cggtcttgcc atgttgatgt ggatggaacc agcaggtagc
    20041 ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa taattccctg
    20101 cgggccgccg tccacttcac ccggtacttc cagcccgtgc cagtgcaacg tcgtctcttc
    20161 cgtcagttgg ttgtagatat caaccgttac cgctttgccg cgctgtaatt tcaccgccgg
    20221 ccccagcaga ttgccgttat agccccaggt agttgcggtt ttcccgccaa aggtggactg
    20281 acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca aatcagggat
    20341 cggtaacgtt gggcgttccg ccgcaaatac tgcgcggctc cacagcggca aagccgaagc
    20401 cacgcccagc gcgacggaat attttaagaa atcacgacgt tgcatagtta tttccttatt
    20461 cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac gggaaggtca
    20521 agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag tgtgctaacg
    20581 tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac agtgttattc
    20641 ggcagcctga tggccgtctg cgcaaacagt tacgcgctca gcgagtctga agccgaagat
    20701 atggccgatt taacggcagt ttttgttttt ctgaagaacg attgtggtta ccagaactta
    20761 cctaacgggc aaattcgtcg cgcactggtc ttttttgccc agcaaaacca gtgggatctc
    20821 agtaattacg acaccttcga catgaaagcc ctcggtgaag acagctaccg cgatctcagc
    20881 ggcattggca ttcccgtcgc taaaaagtgc aaagccctgg cccgcgattc cttaagcctg
    20941 cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg tgcatccgcg
    21001 gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag gaggtatcaa
    21061 cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg gcagtacttt
    21121 gcggtagata acgttctgta tcatgaaaag accgatcacc aggatctgat catttttgag
    21181 aacgccgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac cgagcgcgac
    21241 gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg tcacgcgaaa
    21301 catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac ccgacataaa
    21361 aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtgtcgtt ctgccgtcag
    21421 tatctaccca accataacgc cggtagctac gacgatccgc gctttaaact ggtgatcgac
    21481 gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat ttccgactgc
    21541 accgatccta tcggtcccgg cgaaagtctg ttcacctcag cattttatga aggctgcaaa
    21601 cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt tttacagcag
    21661 gaagaggcca tcgacagcca tcgcaaactc agccattact tcagcgatgt tggcttttat
    21721 caggcggcga tcccgaccta ttacggcggc atcatgacct ttgcatgggc gacagataac
    21781 gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc ttctggcctg
    21841 aaatgccgtt attacaatcc ggcagtccat acggcagctt ttgccttacc ccagtatctg
    21901 caagatgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa aaaactgaaa
    21961 ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga tatctgctac
    22021 gccaaaacta ccgaagagcg cgacggttat attgcttata tcgatgaact ctataatgcc
    22081 aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa tattcttaac
    22141 atcgcccgcc aggattacga gccacaaggt gccagcgtca ctattctggt gagtgaagaa
    22201 ccggtcgacc cgaaacttat cgacaaaaca gaacaccccg gcccactgcc agaaacggtc
    22261 gttgcccatc tcgataaaag tcatatttgc gtacatacct acccggaaag tcatcctgaa
    22321 ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt gatttctccg
    22381 ctgaaggcgc tgaattacct gatccaccag cttgagtccg atattgtgac tattgattat
    22441 cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc actttatcga ccatgagatt
    22501 aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat ggtggatgta
    22561 aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt cgaccttaag
    22621 cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca ggaaattacc
    22681 actgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc cgctgtttaa
    22741 cgtctcaggc ggagcacgga ggctccgcct gatttactta cttctgctgc acgaaattac
    22801 ggtaagccgc caccacctga agaaaatcct caacgccgca cagcgacaag ctctcttcat
    22861 cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga ttggcgcgaa
    22921 ccatcacctc ttcaccatcc atccatagcg tgtattcatg ccctgcccgt tgccaggaac
    22981 gttcgctacc tttcaatgcg tgcgccgctt gttctacttc atcaagcaag gccaggtttt
    23041 ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac atgcgcacct
    23101 ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc tctgctcctg
    23161 gcaatgcgcc gaaatcgcat tgcgccattg atgtaattat cgcagcctta ccggagaaaa
    23221 acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt caccgggcat
    23281 tgtgtcgttt atgcgcagcg cgtgcgctta cttattaaac cgcagtctgg aaaatcaccc
    23341 cgtccgcttt ttcggtgtac tgagaaagct ggtcgaagtt cagataacgg taagtatcaa
    23401 cggctgtttt atctacctgc gccacgtagg tctggtactc ttccggcgtc ggcagtttgc
    23461 caatcagcgc cgcaacagca gccagttccg cagaagccag gaagacattc gcgccagtac
    23521 ccaaacggtt cgggaagtta cgggtagagg tggaaaccac cgtcgcaccg tctgctacac
    23581 gcgcctggtt acccatacac agggaacagc cagggatctc gatacgagca ccactcttac
    23641 cgaagacgct gtagtagcct tcttcggtca gctgcgcagc gtccatacgc gttggcggtg
    23701 ccacccacag gcgggtcggc aactgacctt tgtgcgcatc cagcagttta ccagcagcac
    23761 ggaagtgacc gatgttagtc atgcaagaac cgataaatac ttcgtcgatc ttctcgccct
    23821 gtaccgcaga cagcggacgt gcgtcgtccg ggtcattcgg tgcacagagg attggctctt
    23881 tgatatccgc cagatcgatg tcgatcactg ccgcgtattc cgcatctgca tcggcttcca
    23941 gcagctcagg attcgccagc catttttcca tgccctgaat acgacgctcc agggtacgac
    24001 gatcgccgta accttccgcg atcatccact tcagcaggac gatgttagag ttcaggtatt
    24061 caatgatcgg ttctttgttc agcttgatgg tacaaccggc agcagaacgc tcggcagacg
    24121 catcggtcag ctcaaatgcc tgctcaactt tcagatccgg cagaccttca atttccagaa
    24181 tgcggccaga gaagatgttt ttcttacctt tcttctcaac ggtcagcaga ccttgtttga
    24241 ttgcatacag cgggatcgcg tgtaccagat cgcgcagggt gatacccggc tgcattttgc
    24301 ctttgaagcg caccagaacg gattctggca tatccagcgg cattacgcct gttgcggctg
    24361 caaatgccac cagaccagaa cccgccggga aagagatacc gatcgggaaa cgggtatggg
    24421 agtcaccacc ggtaccgaca gtatccggca gcagcatacg gttcagccag gagtggatta
    24481 cgccgtcacc cggacgcagc gacacaccgc cacggttcat aatgaagtcc ggcagcgtgt
    24541 ggtgcgtgtt aacgtcaact ggtttcggat acgccgcggt gtggcagaaa gactgcatca
    24601 ccaggtcagc cgagaagccc aggcacgcca ggtctttcag ttcatcacgg gtcatcgggc
    24661 cggtggtgtc ctgggaacct acagaagtca ttttcggttc acagtacgcg cccggacgaa
    24721 tgcctttcac gccacaggca cggcctacca ttttctgcgc cagcgagaag ccgcgatcgc
    24781 tctcagcgac atctttcgcc tgacggaaca catcgctgtg cggcaggcca agtgcttcac
    24841 gcgctttggt ggtcaggcca cgcccaataa tcagcggaat acggccgcca gcacgcactt
    24901 catcaatcag cacgtcggtt ttcagttcga aggtcgccag cagttcgccg gtttcgtggt
    24961 tacgcacttc acctttgtac gggtaaacgt caattacgtc gcccatgttc aggttagaga
    25021 cgtcgacttc gattggcagc gcacccgcgt cttccatcgt gttaaagaag atgggtgcaa
    25081 ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga atatcatcgc
    25141 ccataaacca cagaacggag ttagtggcgg atttacgcga tgaccccgta cccacaacgt
    25201 caccgacgta cgccagtggg aaacctttct gttgcagagc ttcgatttgc ttaatcggac
    25261 caacaacgcc tggctgatct ggctcaatac cttcacgggc gtttttcagc atcgccagcg
    25321 cgtgcagtgg gatatccggg cgtgaccacg catccggtgc cggagagagg tcatcggtgt
    25381 tggtttcgcc tgtgactttg aagacggtaa cggtcagttt ttcagccagc gccgggcgat
    25441 tcaggaacca ttcggcatcc gcccaggact gcattacctg cttcgcatat tcgttgcctg
    25501 ctttcgcttt ctcttctacg tcatagaagt tatcgaacat cagcagcgtg tgagaaagtg
    25561 ctttggcagc gatcggtgcc agtttggcat catccagcgc gtcgatcagc ggatgaatgt
    25621 tgtaaccacc ctgcatggtg cccagcagtt cgatggcttt ttccggagtc agcagagggg
    25681 atttggcttc gcctttagcg atagcagcca ggaagcctgc ttttacatag gcggcttcat
    25741 cgacgcctgg gggaacacgg ttggttaaca gatctaacag gaattcttct tcgcccgcgg
    25801 gcgggttttt cagcagctct acaagcgcgg ccatttggtt tgcatccagg ggtttgggcg
    25861 caatcccctc agcggcacgc tcagctacgt gcttacggta ttcttctagc acgacggttc
    25921 tcctcgctct cattgtcata gtgcggcagg cgattctctt cacgctcctg tgagacagca
    25981 gtttgtaggg taaatgccca gtaccgcaac gggcagaata gcaggatttt gacgatgtgt
    26041 taatctgttt acaaaaaagc aacataaaaa agtggctgaa tcgttaagga tggtctagag
    26101 attgtttccc tccgtaaaaa cttcgttaat tcgcatggtg ataatcacct ttctcaacga
    26161 atcccaagat tagtcaaaat ttaaactacc gcctctttat actcggattc acagcacctg
    26221 cgggtggcag ttcgccgacc attgcgattt ccttgagatc cgaattatga aaatgacttt
    26281 gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc gggctttgtg ccgcctctac
    26341 cgggctatat ataaaaagcc gcacagtgga agcgcctgtg gaaccgcaat cgacacaact
    26401 ggctgcgcct gacatctccg cagttacgct tcctgcaacg gtttccgtgc ctcctgtaac
    26461 acctgccgtc gtcaaatccg cattcagcac tgcgcaaata gatcaatggg ttgcacctgt
    26521 cgcgctatac cccgactctc tgctttcaca agtgttaatg gcatcaacct atccggcaaa
    26581 cgttgctcaa gcagtgcaat ggtcgcacga taatccactt aaacaaggcg atgctgctat
    26641 tcaggcggta tctgaccagc cctgggacgc cagcgtcaaa tcactggtgg cctttccaca
    26701 gttgatggca ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg atgcttttct
    26761 ggcgcagccg caggaggtga tggactcggt acagcgattg cggcaactgg cgcaacaaac
    26821 cggctcgctg aagtcatcaa ctgaacagaa agttattacc acaacgaaga aagctgtacc
    26881 ggtaaaacag acagtcacgg ctcccgtcat accatccaat accgtttcaa ctgccaaccc
    26941 tgtcattaca gagcctgcaa caaccgtcat ttccattgaa cccaccaatc ctgatgtggt
    27001 ctatattccc aactacaacc caaccgtggt ttacgggaac tgggccaata ctgcgtatcc
    27061 gccggtttat ctgccgccgc cagccggaga accgtttgtt gacagctttg tgcgcggatt
    27121 cggctatagc atgggcgttg ctaccacgta cgcgctattc agcagcatcg actgggatga
    27181 cgacgatcat gaccatcatc atcatgacga tgatgattat caccatcacg atggcggtca
    27241 tcgtgatggt aatggctggc aacacaatgg cgacaacatc aatatcgacg tcaacaattt
    27301 caaccgtatc accggtgaac atcttactga taagaatatg gcatggcggc acaatccaaa
    27361 ctaccgtaat ggtgtgccct atcatgatca ggatatggca aagcggtttc atcaaaccga
    27421 tgtcaacggc ggaatgagcg ccacgcagct acctgctcca acacgcgaca gccagcgtca
    27481 ggcggcagca agtcagtttc agcaacgaac acacagcgca ccagtcatta cacgagatac
    27541 ccaacgtcag gcagcggcac agcggtttaa tgaagctgaa aactatggga gctatgacga
    27601 cttccgcgac ttcagccgtc gccaaccact gacccagcaa caaaaggacg ccgctcgtca
    27661 gcgttatcag tcggcctctc ctgagcagcg ccaggcagtc cgcgagaaaa tgcagactaa
    27721 cccgcagatc cagcagcgaa gagaggcagc gcgtgagcgc attcagtctg caactcccga
    27781 acagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaatcaac agcaacgtga
    27841 taacgcccgc cagcgtgttc aatcagcatc acctgagcaa cgtcaggttt ttcgggagaa
    27901 agttcaggag agccgcccgc aacgtctaaa cgacagtgac catactgtca ggctgaataa
    27961 cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc gactggaaag
    28021 gtaaattaca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg ccagatgttc
    28081 cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcggt aatgctacct
    28141 tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc atggatggtc
    28201 agtgcgatgt cttcagcgtc acaacccatt tcgattgcca ggccgatttc acccagcaac
    28261 tcgccgccgt tagtgccgac aatcgcacca ccgatcacac ggtgagattc tttgtcgaaa
    28321 atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga agcagcccac
    28381 gggaaggtgg cggtttcgta gctgatgcct ttctctttcg cttctttctc agtcagacct
    28441 acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg atcgaagtaa
    28501 tgttttttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc tttgtgtgcc
    28561 agcattggct gaccgacgat atcaccgata gcaaagatgt gcggtacgtt ggtacgcagc
    28621 tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt gcctgcgtcg
    28681 aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta acgctgcggt
    28741 tcagcgggtg cttttttgcc ttccatcgtc acataaatgc cgtcttcttt cgcttcaacg
    28801 gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt ggtaaagact
    28861 ttaacgatgt ctttatcagc tgccgggatg acctggtcga acatttcaac cacgtcaatc
    28921 tgtgaaccca gcgcgtggta tacggtgccc atttccagac cgatgatacc gccacccatt
    28981 accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc ccagatacgc
    29041 ggatcttcat gcggaataaa cggcagttga atcgggcgag aacccgctgc aatgatcgcg
    29101 ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt gttagccccg
    29161 gtaaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat accagccaga
    29221 ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc gatatcggtt
    29281 ttcggttcgc caaagacgat accgtgttca gccagcgctt tggcttcttc gataactttt
    29341 gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac accgccaagg
    29401 gtgttgtagc gttctacgat tacggtttcc agacctaaat cagcgcaacg gaaggcagcg
    29461 gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc agtactcatc
    29521 atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc cgcagcggcg
    29581 ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga ttccggcgct
    29641 tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct aaccaccacc
    29701 gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac aacaaacggc
    29761 aaccgatttg tctattcgct aataacctca atacattcat gagattacca gaaaaaagcc
    29821 ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga cagcgtgttg
    29881 ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg gtcgaaggag
    29941 agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac cggctccatc
    30001 gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg
    30061 gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat ttcgcccgca
    30121 gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga cagctcgatg
    30181 atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg ggtatccacc
    30241 gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc cgacagcgaa
    30301 ctgttgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat gaagacaacc
    30361 ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg tttacggaac
    30421 gcttccagct cggtgatatc ggttttgtcg aagtgggtaa catgcgggat catcacccag
    30481 ttacggctca ggttcgcacc agagattttc tggatacggc ccagttccac ttcttcgatt
    30541 tcaccaaact tgctgaagtc caccttcggc cacggcagca tgcccgggat accgccgcca
    30601 gtcgccgccg gagccgcttc tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct
    30661 tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt aacgccaaat
    30721 tcgcgtgcca gacggcggat cagcggagtc gcgtgaacgt aagcgtcgtt ttcagcaaat
    30781 tcagatttgc cttcggcttt cgcagccgga gctgctgctg gagcttcagc ttttgctgcc
    30841 ggagccggcg ctgccgcttc ctgtttcgca ggagctgccg caggcgctgc gccttcaact
    30901 tcgaagatca taatcagcga gccagttttc actttatcgc caacgttgac tttcagttcc
    30961 ttcacgacgc ctgcaaacgg agccggaact tccatagacg ctttatcgcc ttctacggtg
    31021 atcagtgact gttcagcggc aactttgtcg cccactttca ccatcacttc ggtcacttca
    31081 acttcgtcac cgccgatatc cggaacgtta acgtctttca cgccagccgc tggtgcagag
    31141 gccgctgccg gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg
    31201 acttcgaaga ccataatcag cgagccggta gacactttgt cacccacgtt cactttgatc
    31261 tctttcacgg tgccagcaaa cggagccgga acttccatag aagctttatc gccttctacg
    31321 gtgatcagcg actgttcagc ttcaacttta tcgccaactt tcaccaggat ttcggtcact
    31381 tcaacttcgt cgctgccgat atccggaacg ttaacgtctt ttgccgctgc agccgctgga
    31441 gctgctgccg gagcggcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca
    31501 ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag
    31561 actttgatct ctttaacgat acccgcctgc ggagacggaa cttccataga ggctttgtcg
    31621 ccttctacgg tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc
    31681 tcggtgattt caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt
    31741 cttttacctc ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga
    31801 ttgcgtcagc aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg
    31861 caaccacgac ataagaagca tcaacctcga agtggtgacg caggttctca cggctgtcgg
    31921 aacgaccgaa gccatcagta cccagtacgc ggtagtcgtc agccggtacg taagtacgga
    31981 cttgctcagc gaacagtttc atatagtcgg tagatgccac tgccggagcg tcgttcatca
    32041 cctgagcgat atacggaacg cgcggagttt ccagcgggtg cagcatgttc cagcgttcac
    32101 aatcctgacc atcacgcgcc agttcggtga aggaagtcac gctataaacg tcagaaccta
    32161 cgccgtaatc tttcgccagg atctctgctg cttcacggac gtgacgcagg atagaaccgg
    32221 agcccagcag ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt
    32281 tacggatacc ttcctcagca ccttccggca ttgctggcat gtggtagttt tcgttcagcg
    32341 tagtgatgta gtagtaaacg ttctcttgtt tctcaccgta catacgctcc agaccgtcat
    32401 gcataatgac agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcgggatag
    32461 tcagcgactg aatgtggctg tgaccatctt cgtgctgcag accttcgccg ttcagggtgg
    32521 tacgaccgga agtaccgccg atcaggaagc cacgcgcttg ctggtcacca gccgcccagc
    32581 acagatcgcc gatacgctgg aagccgaaca tcgagtaata gatgtagaac ggaatcatcg
    32641 gcagattgtt ggtgctgtaa gaggtcgccg ctgccagcca ggaacaccca gcgcccagct
    32701 cgttgatccc ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct
    32761 cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca
    32821 gaccttccat accgaaagta cgcgcttcgt cggcgatgat cggtaccaga cgatctttga
    32881 tcgacttgtt cttcagcatt acgttcaggg cacgaacgaa agcgatagtg gtagagatct
    32941 ctttgctctg ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct
    33001 cggtgaagtt cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgagcgtgca
    33061 gataggtatg ctcttcagaa ccttccggga aggtgatgta cggcaatttt tcgatatcgg
    33121 catcagacac cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc atgttcattt
    33181 tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg taacctttaa
    33241 tggtatgagc aaggattacc gtcgctttgc ctttggtttc ctgcgctttc ttaaatgcag
    33301 cgtagatttt cttcggatcg tgaccaccac ggttcagtgc ccagatctgt tcgtcagtcc
    33361 agtctgcaac cagtgctgcg gtttcaggat atttaccgaa gaagtgttca cgaacgtacg
    33421 caccatcttt cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc atcagctgga
    33481 tcagtttacc gctggtatct ttacgcagca gttcatccca acggctaccc cacatcactt
    33541 tgatcacgtt ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt
    33601 taccggtgac cgggccgtca agacgctgca ggttacagtt gataacgaaa accaggttat
    33661 ccagtttttc acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac
    33721 cgtcgccgag gaacgcgtaa acggtttgtt tagaggtatc tttcaggcca cggtgttcca
    33781 gatatttcag gaatttagcc tggtaaatag caccaatcgg ccccagaccc atagatacgg
    33841 tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc
    33901 cgtgaacttc ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag
    33961 cacgagcgta aacgcccggg gagatgtggc cctggaagta aaccaggtcg ccgccatcct
    34021 gctcgttgcg tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact
    34081 ggaaggacgc catgtggccg cccagttcga ggtctttttt cgacgcacgc agaaccgtca
    34141 tgatggcgtt ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat
    34201 actccggttg ttcttcaacg gggatggtgt tgatgtagtt gctgataccg gtgcctgcgg
    34261 ctacgttgac accgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac
    34321 gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga
    34381 tcggatccac gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac
    34441 gttgagtttt ctggaacctg tcccattgaa ctctcgccgg aaagctcaat aagacaggtt
    34501 ctacgtttag ttgccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt
    34561 taccagaaaa atcactaatt ctttcgttgc tccagacgac gtagagaacg ctcacggcgg
    34621 ctctcttcac ggcttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat
    34681 gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt caaatatgcg ggtgcggtga
    34741 ctactcacca gcggcagcat ctcgcgacgc gaatagagca attcgaagtt ctggcggaca
    34801 ttctgggcca acatcggctc catacacctt agcagatgaa gcagaaccac attgtgggcc
    34861 gcttcggtga cggcaatctg atactggagt acggcatttg attccgcgtc cagatcgcca
    34921 gactgctgcg ccagctctat ggcatggtgg agttcacgga tgcgttcctt gtcttcatcg
    34981 gtactacgca gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc
    35041 aagtcatact gtgattcagg atggtcggag agcagctcca ccagcggatc gctgaagctt
    35101 tgccataggc tgctctggac aaaagtgccg ccaccctggc gacgaagcaa caagcccttc
    35161 gcttcgagac gttgaatcgc ctcacgcaag gagggacggg agacgtcgaa ctgttttgcc
    35221 agttcgcgtt ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac
    35281 tccagttgct gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg
    35341 agttcctgtc ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac
    35401 gctaaagtaa caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact
    35461 gtgcacattt taacaacttg acatatataa cgtttcaaag ttgtaactat gcacaaatgt
    35521 taaatttacc catcaagggg tgagattaac agaattgaaa cgaaaaaata tgtcctctga
    35581 accgattcac caagcaacaa aacgcataac aattcaatta tgtgaattaa tatgtaaaat
    35641 gaatgttcag ggaaggagag aaaggtagta tttacctatg ttttcttttt acattgctta
    35701 ttatcactgc aatcgccacc gtaaccttct cgtagcgaaa atcatcactt aaccagaacg
    35761 tgatgatttt caggtgacag attctgttta gaattcattc tgaatctttg aacaaagaca
    35821 atccttgcga acgccgccat tctttgacga ttttcaggat gttttcaggc ttatcatctc
    35881 ccttctttgc aggatagtag ataagatcgc tcatcaacgg atgttgtgta attctggtaa
    35941 gatgttcttc tagatcatcc caatatttgt caagctcttc cccttttaat tgagcgttat
    36001 ccccggttgg atttgtaaac tcgccaagca actcaatgaa ttcccgctct gtgtaatctt
    36061 ctactttatc tttaaaatta tacatttaac tatctcctgt aataaatttc atcatgcaaa
    36121 tgaggtgtgc cctccttaaa ttatcaatat cgtaaatttc gccactatat tcaatcgcaa
    36181 cacaatgata catgtggaat tttttaacga tttctttagg gccataatgc caaccttcaa
    36241 gaggtgcttt tgcgattaat ccttgtttta tacgtgtttg attaccactt gaaaactgcg
    36301 ctatcaactc cggatactga ctcacctcca gccaaagggt atc
//
LOCUS       DAZGAP010000021        35754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGAP010000021 DAZGAP010000000
VERSION     DAZGAP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.021"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..163)
                     /locus_tag="ACHLAM_000918"
     CDS             complement(<1..163)
                     /locus_tag="ACHLAM_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO7852488.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIET"
     gene            complement(290..1021)
                     /gene="rlmB"
                     /locus_tag="ACHLAM_000919"
     CDS             complement(290..1021)
                     /gene="rlmB"
                     /locus_tag="ACHLAM_000919"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO7852489.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1112..3553)
                     /gene="rnr"
                     /locus_tag="ACHLAM_000920"
     CDS             complement(1112..3553)
                     /gene="rnr"
                     /locus_tag="ACHLAM_000920"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO7852490.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3592..4017)
                     /gene="nsrR"
                     /locus_tag="ACHLAM_000921"
     CDS             complement(3592..4017)
                     /gene="nsrR"
                     /locus_tag="ACHLAM_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO7852491.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4222..5520)
                     /gene="purA"
                     /locus_tag="ACHLAM_000922"
     CDS             complement(4222..5520)
                     /gene="purA"
                     /locus_tag="ACHLAM_000922"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO7852492.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5624..5821)
                     /gene="yjeT"
                     /locus_tag="ACHLAM_000923"
     CDS             complement(5624..5821)
                     /gene="yjeT"
                     /locus_tag="ACHLAM_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO7852493.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5903..6907)
                     /gene="hflC"
                     /locus_tag="ACHLAM_000924"
     CDS             complement(5903..6907)
                     /gene="hflC"
                     /locus_tag="ACHLAM_000924"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO7852494.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6910..8169)
                     /gene="hflK"
                     /locus_tag="ACHLAM_000925"
     CDS             complement(6910..8169)
                     /gene="hflK"
                     /locus_tag="ACHLAM_000925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO7852495.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8255..9535)
                     /gene="hflX"
                     /locus_tag="ACHLAM_000926"
     CDS             complement(8255..9535)
                     /gene="hflX"
                     /locus_tag="ACHLAM_000926"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO7852496.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGEGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9612..9920)
                     /gene="hfq"
                     /locus_tag="ACHLAM_000927"
     CDS             complement(9612..9920)
                     /gene="hfq"
                     /locus_tag="ACHLAM_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO7852497.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10006..10956)
                     /gene="miaA"
                     /locus_tag="ACHLAM_000928"
     CDS             complement(10006..10956)
                     /gene="miaA"
                     /locus_tag="ACHLAM_000928"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO7852498.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10949..12796)
                     /gene="mutL"
                     /locus_tag="ACHLAM_000929"
     CDS             complement(10949..12796)
                     /gene="mutL"
                     /locus_tag="ACHLAM_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO7852499.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTVEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKMVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPLYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12806..14143)
                     /gene="amiB"
                     /locus_tag="ACHLAM_000930"
     CDS             complement(12806..14143)
                     /gene="amiB"
                     /locus_tag="ACHLAM_000930"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO7852500.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTFLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14162..14623)
                     /gene="tsaE"
                     /locus_tag="ACHLAM_000931"
     CDS             complement(14162..14623)
                     /gene="tsaE"
                     /locus_tag="ACHLAM_000931"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO7852501.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14595..16142)
                     /gene="nnr"
                     /locus_tag="ACHLAM_000932"
     CDS             complement(14595..16142)
                     /gene="nnr"
                     /locus_tag="ACHLAM_000932"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO7852502.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16141..17280
                     /gene="queG"
                     /locus_tag="ACHLAM_000933"
     CDS             16141..17280
                     /gene="queG"
                     /locus_tag="ACHLAM_000933"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO7852503.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17263..17316)
                     /locus_tag="ACHLAM_000934"
     CDS             complement(17263..17316)
                     /locus_tag="ACHLAM_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852504.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17550..17625)
                     /locus_tag="ACHLAM_000935"
     tRNA            complement(17550..17625)
                     /locus_tag="ACHLAM_000935"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17590..17592),aa:Gly,seq:gcc)
     gene            complement(17661..17736)
                     /locus_tag="ACHLAM_000936"
     tRNA            complement(17661..17736)
                     /locus_tag="ACHLAM_000936"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17701..17703),aa:Gly,seq:gcc)
     gene            complement(17894..17969)
                     /locus_tag="ACHLAM_000937"
     tRNA            complement(17894..17969)
                     /locus_tag="ACHLAM_000937"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17934..17936),aa:Gly,seq:gcc)
     gene            complement(18180..18725)
                     /gene="orn"
                     /locus_tag="ACHLAM_000938"
     CDS             complement(18180..18725)
                     /gene="orn"
                     /locus_tag="ACHLAM_000938"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO7852505.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18820..19872
                     /gene="rsgA"
                     /locus_tag="ACHLAM_000939"
     CDS             18820..19872
                     /gene="rsgA"
                     /locus_tag="ACHLAM_000939"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO7852506.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19969..20937
                     /gene="asd"
                     /locus_tag="ACHLAM_000940"
     CDS             19969..20937
                     /gene="asd"
                     /locus_tag="ACHLAM_000940"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO7852507.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            20959..24282
                     /gene="mscM"
                     /locus_tag="ACHLAM_000941"
     CDS             20959..24282
                     /gene="mscM"
                     /locus_tag="ACHLAM_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO7852508.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24313..24627)
                     /gene="yjeO"
                     /locus_tag="ACHLAM_000942"
     CDS             complement(24313..24627)
                     /gene="yjeO"
                     /locus_tag="ACHLAM_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO7852509.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24624..24938)
                     /gene="yjeN"
                     /locus_tag="ACHLAM_000943"
     CDS             complement(24624..24938)
                     /gene="yjeN"
                     /locus_tag="ACHLAM_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO7852510.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(25001..26503)
                     /gene="yjeM"
                     /locus_tag="ACHLAM_000944"
     CDS             complement(25001..26503)
                     /gene="yjeM"
                     /locus_tag="ACHLAM_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO7852511.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(26722..27699)
                     /gene="epmA"
                     /locus_tag="ACHLAM_000945"
     CDS             complement(26722..27699)
                     /gene="epmA"
                     /locus_tag="ACHLAM_000945"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO7852512.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            28024..29832
                     /gene="frdA"
                     /locus_tag="ACHLAM_000946"
     CDS             28024..29832
                     /gene="frdA"
                     /locus_tag="ACHLAM_000946"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO7852513.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29825..30559
                     /gene="frdB"
                     /locus_tag="ACHLAM_000947"
     CDS             29825..30559
                     /gene="frdB"
                     /locus_tag="ACHLAM_000947"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO7852514.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30570..30965
                     /gene="frdC"
                     /locus_tag="ACHLAM_000948"
     CDS             30570..30965
                     /gene="frdC"
                     /locus_tag="ACHLAM_000948"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO7852515.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30976..31335
                     /gene="frdD"
                     /locus_tag="ACHLAM_000949"
     CDS             30976..31335
                     /gene="frdD"
                     /locus_tag="ACHLAM_000949"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO7852516.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            31398..32531
                     /gene="ampC"
                     /locus_tag="ACHLAM_000950"
     CDS             31398..32531
                     /gene="ampC"
                     /locus_tag="ACHLAM_000950"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7852517.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            32620..33153
                     /gene="blc"
                     /locus_tag="ACHLAM_000951"
     CDS             32620..33153
                     /gene="blc"
                     /locus_tag="ACHLAM_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7852518.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(33154..33471)
                     /gene="sugE"
                     /locus_tag="ACHLAM_000952"
     CDS             complement(33154..33471)
                     /gene="sugE"
                     /locus_tag="ACHLAM_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7852519.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33646..33792)
                     /gene="ecnB"
                     /locus_tag="ACHLAM_000953"
     CDS             complement(33646..33792)
                     /gene="ecnB"
                     /locus_tag="ACHLAM_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7852520.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33903..34028)
                     /gene="ecnA"
                     /locus_tag="ACHLAM_000954"
     CDS             complement(33903..34028)
                     /gene="ecnA"
                     /locus_tag="ACHLAM_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7852521.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(34080..34646)
                     /gene="efp"
                     /locus_tag="ACHLAM_000955"
     CDS             complement(34080..34646)
                     /gene="efp"
                     /locus_tag="ACHLAM_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7852522.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34688..35716
                     /gene="epmB"
                     /locus_tag="ACHLAM_000956"
     CDS             34688..35716
                     /gene="epmB"
                     /locus_tag="ACHLAM_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7852523.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8531 a         9790 c         9058 g         8375 t
ORIGIN      
        1 aggtttcgat aatcatttgg cgggaagtaa atagaatatt gatttgcaaa tcgccataat
       61 cattcatttt aataattaat gcgttctcgc tatttgttac atcaatattt tgttcaggta
      121 cagtttgcag cgccgtcgcc agcgccaacg gattccatgt catatattat tccatataaa
      181 ttaagtttaa atattaataa aatgaatatt tgcagtacgt aattatctta ccagctatag
      241 acaaaaaaaa accatccaaa tctggatggc ttttcataat tcagaggaac tagctgcgct
      301 gacgaaccgc ttcaaataag caaattccgg ttgcaaccga aacgttcagg gaagaaacac
      361 ttcctgccat tgggatgctg atcaactcat cgcaatgttc acgggtcagg cgacgcatac
      421 cttcaccttc cgcgcccatc accagcgcca ggcgtccggt cattttgctc tgatagagtg
      481 tatgatccgc ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc aacatacgca
      541 tggtgcgcgc cagattagtc acccgaatca gcggaacgct ttctgccgca ccacaggcca
      601 cttttttcgc cgtggcgttg agctgtgcgg agcgatcttt cggcacaatc accgcatgaa
      661 caccagcggc gtccgcgcta cgcaggcacg cgccgaggtt gtgcggatcg gttacgccgt
      721 cgaggatcag caggaacggt tgatcgagcg aagcgatcag atccggcaga tcgttttcct
      781 gatactgccg tcctggcttc acacgagcga taatgccctg atgcacggca ccgtcgcttt
      841 tctcgtcgag atattggcgg tttgccaact ggataaccac gccctgagac tcaagggcgt
      901 gaatcagcgg taacagacgt ttatcttcac ggccttttaa aataaagact tcctgaaaac
      961 gttcaggggc gcgctccagc agggcctgca ctgcatggat gccgtaaatc atttcgctca
     1021 ttaatgtact cgttatttac gttttgccgg gagcgccacg cttgcccggc ctacggataa
     1081 ttcaccctct tctgtttgaa gagggtaatg atcactctgc cacttttttc ttcgccgcac
     1141 gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc cgatggcttt ttcgcttttc
     1201 tcgcgtcttt cttcgctgct ttcggcttcg gttttttctc gccgcggaag gcgctgtctg
     1261 gctcaaagtt gacctttttg ccgacctgac gccgcttgcc gccttttttg cctgcatcgc
     1321 cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt tcgctggaga
     1381 tcaggctaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga acttccacgc
     1441 gatcgcccag gcgataagtc tggccgctgg attcgcccat caggcgttgc cccacctggt
     1501 caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca atgaacaagt
     1561 catccaggcg aacaaagaag ccaaaaccag tgacgctgga aattacgcct ttaaagacgt
     1621 tacctacctg gtcgagcatg aagtcacact tcagccagtc agccacatcg cgcgttgctt
     1681 cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcagcatc tcttccatcg
     1741 aataatggta gccgccggtt tcagtggtat tcccctgatg cccctgctct ttcgccaaca
     1801 gatacttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc gaagtaaagt
     1861 gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt ttccggatcg taaatcgctt
     1921 gtttcatcga gcgcagcagc atggtttgca gcatttctgc atcaggacgg tcggcaaccg
     1981 actccagcag ctctgcgtag tcgcgcggtt ccggcttgtt accgcccggc agttccagcc
     2041 ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc ttgtcgtgaa
     2101 tacggaacag tgccggttct ttcgctttct caacgaaacg cgccgccgag atattcgcca
     2161 gaatcatgca ctcttcaatt aacttgtgtg cgtcgttacg ctgagtctgt tcgatacgct
     2221 caatacggcg ttcggcgttg aaaatgaact tcgcttcttc gctctcaaat gagatcccgc
     2281 cacgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct tcgagatgct
     2341 taaccagcgg ggcatactgc tcgcgcagat cctgatcgcc ttgcagtata tgccagactt
     2401 tggtgtaggt cagacgcgcg tgagaactca tcaccgcttc gtagaatttg tagccagtca
     2461 ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct acctgcgggt
     2521 tgagcgagca caggccgtta gagagcactt ccggcagcat cgggataacc tgcgaaggga
     2581 agtacaccga cgtaccacgg ttacgcgctt ctctgtccag cggtgttggc ggacgcacat
     2641 agtaactgac gtcggcaatt gcgacccata aacgccagcc gccgccgcgt tttttctcac
     2701 agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc agcggtaaat
     2761 cgcgcagatc gacacggccc gcttttgctt cttccggcac ttcttctttc agcccggcaa
     2821 cctgttgctc aacagcctgc ggccagatgt acggaatttc atgggtacgc agagcaatat
     2881 caaccgccat gccggtgccc atattatcgc ccagcacttc gacgatttta cccaccgctt
     2941 tggtacggcg agtcgggcgt tgagtcagtt caaccaccac cacaaagccc atccgcgcgc
     3001 ccatgatctg atcgggcggg attaagatat cgaagctcag acggctatcg tcaggaacca
     3061 caaagccgac gcccgcttcg gtgaagtagc gaccaacaat ctggctggtt tttggcacca
     3121 gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcgcccagc ggctgagcca
     3181 gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac aaatcatctt
     3241 tacgcccttc aactcgcaga aagccgtagc catcacggtg gccaataacg gtacctttca
     3301 ccaggtcgag gcgttccggc agcgcatagc actgacggcg agtgaagacc agttgaccat
     3361 cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg ccttcaatgt
     3421 gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt aaatgttcga
     3481 ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt tcctggaaag
     3541 gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc gtcactccac
     3601 cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg tgtagttatc
     3661 cagttccgta agaaaacttt gcacggcctt agaaagcgcc tgtttcaacc gacaggcagg
     3721 tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca gctcgcgcac
     3781 cacatcacca atacgtattg cactcgccgg tttacccaag cgaatgccgc cattttttcc
     3841 acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga ccatatgatt
     3901 acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc cttctggcaa
     3961 tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta actgcacatc
     4021 aacctcaaaa gggaaatcgg ggaaaaatac atttatattg atgataaacc agccacaagc
     4081 tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa cactgtgtcg
     4141 gatagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg gcaggcaaaa
     4201 tatctgccag gcgtaccaga attacgcgtc gaacgggtcg cgcagaatca tggtttcagt
     4261 acgatccgga ccagtagaga tgatatcgat cggcacaccg gtcagctctt caatgcgctt
     4321 gatgtagttc agtgccgcct gcggcaggcc gctacgatct ttcacgccga aggtggattc
     4381 agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt cagctgccag
     4441 cggagtggta gtcacttcac gaccatccgg catacggtaa gccacgcaga gtttaacctc
     4501 tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacagag agttcagctg
     4561 taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac gacccgtagt
     4621 tgcgccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa acagttcggt
     4681 cgggaacgga cctgcaccca cacgagtgga gtaagctttg aggataccca gaacgtaatc
     4741 aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg tgttggaaga
     4801 agttacgtac ggatatgtac cgtggtcgat atccagcagc gtaccctgcg caccttcaaa
     4861 catgacgaaa tcgccacgct gacgcgcctg gtcaagcagg tcagaaacgt caacaaccat
     4921 agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt aatcaaccgc
     4981 ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt ctttcagttt
     5041 ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac gtgccacttt
     5101 atcttcataa gcaggcccga tacctcgacc ggtggtgccg atcgctttcg cgccacgcgc
     5161 tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac atgcttcgga
     5221 cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca tctctttcat
     5281 cagcgcagcc ggagacagca caacaccgtt accgatgatg ctggttacat tctcgcggag
     5341 aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga gagtatggcc
     5401 tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca gaagatcgac
     5461 gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt tgttacccat
     5521 ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca gagttacagc
     5581 actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca atcgttttcc
     5641 tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa cgacgtaaaa
     5701 tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc gggtaaagca
     5761 tcggccctaa accttccagt accaaaacca gggcaagcgc cagccagatt gtcgaattca
     5821 ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg tggctttatt
     5881 gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc atgtagcgga
     5941 agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag ctgttctcat
     6001 aagcacgcag gctacggata aatgcgtaga agtccggatc tttactgaat gcatcagcaa
     6061 acagtttggc tgcttctgca tcgccttcac cacgcatgat gcggccctga cgctcagctt
     6121 cagccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc gcttcttcct
     6181 gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata cggttgtaga
     6241 tcgcttcaga cacttcggtc ggcaggttga tctgcttaat acgcacatcg acaacttcaa
     6301 tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc gtctcagccg
     6361 ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc cggggtagta acttcatctt
     6421 ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga cgaccacggg
     6481 aatcggtgac gatgtctttc acgtccaggc gaccaatttc agaacgcaga cggtcagaga
     6541 acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt gccaggtagt
     6601 aacggctgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg tctttcttct
     6661 ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg tcgagcattt
     6721 tcaccgtttc aatgaacggt atcttgaaat gcagacccgg ctcataaacc agaggtttgt
     6781 tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accgcgctca ccttctttga
     6841 cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg ataactgact
     6901 tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg cgcggcgttg
     6961 gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg ttgtggagga
     7021 agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg ccgcaggggc
     7081 gttaccacct ttcagcatct ggtctaacgg cagaaccatc aagttgccac ctttatcgtt
     7141 aaccagcact ttgcgggtgt tgcccaacac tttttccatc gtctcgatat acagacgctc
     7201 gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc gcgccacttc
     7261 accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga ggatacgttg
     7321 cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt cacgaatgta
     7381 ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta cttcttccgg
     7441 cggacgagct gcctggaagt tgacgtccag cagcgtgata cccatgtcat acggacgaat
     7501 cgtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt ccgtcagaat
     7561 gcggtccatg gtgtatttac cgataacccc acgcagggcg ctgtcggtag cctgacgcag
     7621 gctgtcatcc gggctggtca cgctatacag atatttttcc ggatcggtga cgcggtactg
     7681 cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac cagaagcggc
     7741 cagttcacgc acggcttcca cgttcaccgg tttgacttcg tcgataaacg tcggtttcca
     7801 gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca cgccacgttc
     7861 ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg ctgccgctgc
     7921 gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac tgccaccgcc
     7981 agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt tgcggaagat
     8041 atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc cctcagagtt
     8101 gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac cgggctgatt
     8161 ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct ttcgacgcca
     8221 gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg atcaacgccg
     8281 gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc agacttacgc
     8341 tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa cgacttctca
     8401 gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc cacctcgccg gaaagccgct
     8461 ccgtcaaagc ctgaaaaagc tgtggtatcc cctctccggt ctgtgcggaa agccagacac
     8521 ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc agcatatcga
     8581 tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca agaaccgtat
     8641 tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg tgcagcagca
     8701 atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga tcgtgcggca
     8761 ggtggcgaat aaaccctacg gtatctgcca acacggtttc accgacatct gcgacgtcaa
     8821 tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag acccgcgctt
     8881 cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc agcgaaacag
     8941 taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt tcaactcttt
     9001 ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg gtttcgagct
     9061 gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg tgggtccagc
     9121 cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc aacttaccct
     9181 catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg tcgataacac
     9241 gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca tggtcaaaaa
     9301 gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct ttaccttcac
     9361 ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc aatgcttcga
     9421 caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct ttgtcttgcg
     9481 taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca aacaagcgta
     9541 taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg taaaaaaaac
     9601 agcccgaaac cttattcggt ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg
     9661 ctaccatgat ggtagttact gctggtaccg ccaccggcgt tgttactgtg atgagaaacc
     9721 gggcgagacg ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt
     9781 ttcaacagga tcacgaactg atcaaaagac tcgatttgcc cttgcagctt aataccattc
     9841 accaaataaa tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt
     9901 aaagattgcc ccttagccat tctctctttt ccttatatgc ttatttgtac tttgaacctt
     9961 tcgattctga aaaaattgcg cacgatacgt ctcaattgta cacattcagc ctgcgatagc
    10021 accaacaacc tgtaatactt cgtcacgcgc ttgttctggt ttttcactgt caagccagtg
    10081 aaccccttcc caaccacgca gccaggttat ctgccgcttc gccaactgtc tcgtggcgca
    10141 aacacctcga taaaccattt catcgtatga gatttcgcct tcaaggtaag accacatctg
    10201 gcgataaccc acgcaacgaa tggaaggcaa atccgtatgc aaatctcctc gggcaaaaag
    10261 cgcccggact tctgcttcaa aacctgaagc caacatctga tgaaaacgct gctcaattcg
    10321 ttgatggagc agttcacggc tcgccggggc gatggcgaac tgatgcacct gatacggtag
    10381 agcgtctcct gacgtttgcg tcagttccgt taaagtttta cccgaaatga aaaaaacttc
    10441 cagtgcccgg gaaagccttt gtggatcatt tggatgaatc cttgctgccg caaccggatc
    10501 tacctcctga agttgacgat gcaatgactc ccaaccttgc tctgccgcct gttgctcaat
    10561 tctggcccgc acttccgggt ctgccgacgg tagcggcgac aacccttcca gcaatgcctt
    10621 gaaatacaac atcgtaccgc ccactaacag tgggatccgc cccgccgcgg tgatatcggc
    10681 catttccgcc agcgcatcgc ggcgaaaatc agcagccgag taagcctgcg acggatcgcg
    10741 aatatccagc aatcggtgcg gcgcggcgag taactcttca gcgttcggct tcgccgtccc
    10801 gatatccatc cctttgtaaa taagggcaga atcaacgctt atcaactcta ctggtaaaat
    10861 tttacgcagc tcaatggcta acgccgtttt accggaggcc gtcggcccca tcaaaaaaat
    10921 cgccttaggc aggctcgcct tactgatatc actcatcttt cagggctttt atcgccggat
    10981 gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc
    11041 gttccacgtc cgccagcagg gttatggcct gcgccattga ccactgcgca tgttcgctca
    11101 tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca
    11161 ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca
    11221 cggccctgat ggtcacatgc tgcgcatcgg actggaaatc aatacccaat tccgccaggg
    11281 cagactgcgc tttttctaat gcagattttt cttcgccaga aacttttagc cgcagcggaa
    11341 tcagcaacgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc tgacgcagcc
    11401 aacgttctgc cactggcaag gctaaaagtg aaatgttgcc gtcgcgctcc agcaacgcac
    11461 agtcggaatg gacgatagtc agtacccgac caaagctttg actgttcgcc gcaagtgcag
    11521 gttcctgcgg ttctggcgct tttggttttt gcatcggcgc gggcgtttgc aaaagctggc
    11581 gatacacttc accttgctgt ttctggtaac ctggctgcgc attcggccag ggggcagccg
    11641 gacgactgcc tgaggcgggc gcaggagtgt aaagcggagc tactggctca cgaactgccg
    11701 gttcagcaaa atgattgcgc cccgccgcca cgcggttttc cgggatggcg cgcggtgcag
    11761 gttgtggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca
    11821 cgccctgata gataaagtca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg
    11881 cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc cagatacagc acaaacgccg
    11941 gttgttgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcatga ttgatcaggc
    12001 gatcgcgcat catgcgacca ttcacgtagc aatactgaat ttccgccagt gcgggcgtgg
    12061 tgtgattcgg atctgccacc cagccgcgca gcgtaagatc gccgtgttgc cattcaatcg
    12121 ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcccc taaacggcgc tctttttgtc
    12181 caccttccgg cacggcgcgg tactggcgga ccattttccc gttatgcgac aggttgatcg
    12241 tgacgtcgaa acgcgccagc gcaatgcggc ggatgatctc atcaatgtgg ttaaattcgg
    12301 ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct
    12361 ccagcgtagt ccccacagga tgcgccgccg gttttaccgt cacgtccata tcgcgccctt
    12421 cggcataggc ctgccaggct tcctgctgtt ctacggtgcg tgaagtgagc gtcaggcggg
    12481 aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata atggcttcga
    12541 gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat
    12601 cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgccccg ccacgttcga
    12661 tatcaatatc aatgcgcgtc gcacctgcat cgaggctgtt ttccaccagt tctttgacta
    12721 ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta
    12781 agacctgtat tggcatcaat cgtccttagt ttggcagcgt acgatctggc gtcgtcaccg
    12841 tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg catcggatgc gcaaggaaat
    12901 aattgcgcag acctttataa atggcttctg ccagctgttg ttggtaatcg tcgctcgcca
    12961 gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc gaccagtact gatgggatat
    13021 ccggcgaacg cagaacgcca aggctggcgt gttctggtcg acgtttatgt atttcgccaa
    13081 tgcgttgcaa ctggctgatc atactggtcg ctacatcata ccctacacgc tgggaatgac
    13141 cgaactgtaa atccagcacc gcctggctta aatagggatc agactgactg ttcgccagca
    13201 catcgcccgc tccgcccagc agctccgact gtttctcgtg ctgctccagc cagctggcca
    13261 tttcgctgtt ggcgcgacgg ttagagagca cccatacgga agcgccagtc gcactgcggt
    13321 ttggtgcggc atcagcgtga atcgacacga ggaaattggc gttttgctta cgtgccacat
    13381 cgctgcgccc catcaccgag ataaagtaat ccccgtcacg ggttaaaacg cctttaaaca
    13441 ttggatcgtc attgagaaaa gtacgcaatt tacgtgcgat ggcgatggtg acatttttct
    13501 cccgcgtacc accggggccg atagcgccag ggtcctgacc gccgtgtcct gcatcaatag
    13561 cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc tgccggacgc gttaccgtat
    13621 tactgctgat aacacctgta gtgcggttac tttccgtttt aaacggattg cgcgccggtt
    13681 cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg tttagcaacc acaggcggcg
    13741 aaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat
    13801 tctgccgctt caccgcttcg gttttaccgt tttcggttag atcgaccacc agccgtagcg
    13861 tttgtgcatc tttaggcgtt ccggagcgaa tcgccttcac cagattattg ccgctaaaca
    13921 acaacggcag tccctgaatc acgcccgttt gtttgatatc gagcgccacg gtgcgtttgc
    13981 tttgatggct aaacgcataa tcaggatcgc caataaaact caacgttatc cgcgcctgct
    14041 gattgccgtt agaaacctga atatcagaga gcgtcgcggc acccaccggc gcgcacagca
    14101 gcagcaacgt cgctaccaac caatttctga tgcgatacat catcccgcca cctttcaaag
    14161 gttaaccggc taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg
    14221 cctcacggcc ttgtgcctga taatcaatgt gtatttcgac atccgggtca ggaagaacac
    14281 ctgtaccttg ttgtggccac tccaccaggc agatggcatc gttggcaaaa taatcgcgga
    14341 tccccataaa ctccagctcc tcgggatcgg caaggcggta caaatcaaag tgataaacca
    14401 ttaaattgtc gagcgtatag ggttcgacca gcgtataagt ggggcttttg acattaccct
    14461 gatggcccaa agcctgcaaa aagccccggc taaaggtggt tttacctgcg cctaaatcgc
    14521 catacagata gattacggtt gcgccatcgc aggctttcgc tacccgctcg cccaggtcta
    14581 atgttgcctg ctcatcaggg agcggaatta ctcgattcat catggttttt atcagtcact
    14641 tccgggttaa caatacgctg tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt
    14701 ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg cgacacagcc cgcacaggct
    14761 gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa taataccaga gagcacatcg
    14821 cccatgccgc cgctcgccat gcctgcattc ccggcatcaa taatgcctaa agcgtcagga
    14881 tgggcggcga ccacggttcc ggcacctttc agcaccgcta cgccgccata acgttgtacc
    14941 agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca gcctaacaac
    15001 cgtgcggcct cgccaggatg tggcgtgatc acgcgattgt gacgcttatc gggattgatt
    15061 gccagcaggt tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt
    15121 tgcagggctt ttttccccca ctcttgctgg cccagaccgg gaccaatcac caccacatcg
    15181 gcccattcca ggctttcggt aagagagtcc atagtcagtt catgcaccat caattctggt
    15241 cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca gtactcggac taaaccagca
    15301 ccagcacgta gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca
    15361 ccgataatca ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg cggtatcagc
    15421 cactgagaaa gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc tgccagccag
    15481 ctatccagcc ccagtgagtc aaaatgcagt tgtccggtaa catcccgcgc ttttccagtg
    15541 agcaagcctg gtttcagcgc aataaaagtg atggtgtgat cggcgttgat taccgcgcct
    15601 ggcgtagcgc cggtttcagc cagaaggccg gaagggatat caaccgccac aatcggcgca
    15661 gggtgagtat tagcgtggtc gattaactgg ctaatggatt cgcggggcgc ttgctgcaag
    15721 ccggtgccga gcagcgcatc aacaatcaga tctaccgatt cgggccagac aatattcgaa
    15781 gcatggatct cgcctcccgc gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc
    15841 aacggtttgt cactttcctg ggccagcagc gtgacatcaa taccgatcgc tttggccagc
    15901 cgcgcgacca cgtagccatc gccgccgtta ttaccatgac cgcacagcac cagccagtgg
    15961 cgggcgtcag gataagccga acgacacacc tggaatgcgg cttcgccagc gcgaagcatc
    16021 agctcataga gtgtgagccc cagcgcatct gccgcctcgc gttctccgcg gcggatatcg
    16081 tcggcgtacc agacggtgtg tggtatactt acggggtttt tcttcattgt atggtccgtc
    16141 atgtcagagc ccctcgatct caatcaatta gcgcaaaata ttaaacagtg ggggctggaa
    16201 ctgggctttc agcaggtagg tattaccgat accgatctca gcgcgtccga gcccaaactg
    16261 caagcatggc tggacaaaca ataccacggc gaaatggact ggatggcgcg tcacggtatg
    16321 ctgcgtgctc gtcctcacga attattgccc ggtacgctgc gcgtgatcag cgttcggatg
    16381 aattaccttc ctgctaacgc cgcatttgcc agcacgctga aaaatcccaa actcggctat
    16441 gttagccgtt atgcgctggg ccgtgactat cacaagcttc tgcgcaaccg actcaaaaag
    16501 ctgggcgaga tgattcagca acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct
    16561 gcgcctattc tcgagcgccc gttagctgaa aaagcaggtc ttggctggac aggtaagcac
    16621 tcacttatcc tcaatcgcga ggccggttcg ttcttctttt taggcgaatt gctggtcgat
    16681 attccgctgc ccgtggatca accagtcgag gaaggatgcg gtaaatgcgt ggcctgtatg
    16741 acgatttgcc cgaccggtgc catcgtcgag ccatataccg tcgatgctcg ccgctgtatc
    16801 tcttatctca ccatcgaact ggaaggggcg atcccggaag agttgcgacc gttaatggga
    16861 aaccgtattt acggttgcga tgactgccag cttatctgcc cgtggaatcg ctattcgcaa
    16921 ctcactacag aagacgattt cagcccgcgt aagccgctac acgcaccgga actcattgag
    16981 ttattcgcct ggagtgaaga gaagttttta aaagtcacgg aaggttcggc gattcgccgt
    17041 ataggtcacc tgcgttggct gcgtaatatc gccgtagcat taggcaatgc cccctgggat
    17101 gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc acccacttct tgatgagcac
    17161 atagcgtggg cgattgcgca gcaaatcgag aggcgaaatg cgtgcgtggt cgaagtgcaa
    17221 ttacccaaaa aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga
    17281 ttcatccaca gcttgtgaat aaaaataaaa acgtattgca attcaagacg aataaaatcg
    17341 tcaagtgatc gactcgacaa aacgaaatct taatttttaa cttatctatc aataggttaa
    17401 aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag attacggcct gaggctgtgg
    17461 ataactctgt ttacaagagt ttgtcagaga caacaaaaac cagcaataac gcatcgcgtc
    17521 gctgtggata ttttattgag agaagaattt ggagcgggaa acgagactcg aactcgcgac
    17581 cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcttggg tggtctgtgc
    17641 tttacagcac tttcaaattt tggagcggga aacgagactc gaactcgcga ccccgacctt
    17701 ggcaaggtcg tgctctacca actgagctat tcccgcttaa tcttcgtctt tcgaaccgct
    17761 actgccttgg ctgccttcac tcaccttagt cacttacttt tgtaagttcc caaggattcg
    17821 ttcagttgcc gccttgtcgc aattcgaaat actgcgattg ggtggtctgt gctttgcagc
    17881 acttttcaaa ttttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    17941 tcgtgctcta ccaactgagc tattcccgcg tactacttaa ttttgctttc gtaattttcg
    18001 catttctgcg tcgttacggg aggcgcatta tacgagaaat ctttccctct gcaacccccc
    18061 taaaaaattt tttttgagtt tttatctcaa gtgctgaaat tatcagcaag acgttcaatt
    18121 tagcgtcaaa atcacgccgc cgcaagtgga ttccggcgct tacgtgacca ggaaaaattt
    18181 tacagcttga taaaatgctc gcggtagtaa gccagttctg ccaccgactc acggatatca
    18241 tccatcgcct gatgcgtccc ctgcttggta aaaccatcaa gaatttccgg cttccagcgg
    18301 cgcgccagct ctttcagggt gctgacatcg agataacggt agtggaagta ggcttccagc
    18361 tccggcatat atttaaacag gaagcgacga tcctggccga tgctgttacc gcaaatcggc
    18421 gattttcccg caggcaccca ctgttttaag aattcgagcg ttgccagttc agcttcacga
    18481 tcgcccatcg tgctcgcttt cacgcgctcc accagcccgc tggcggtatg ggtgcgcacg
    18541 ttccagtcat ccatcagcgc cagctgttca tcagactggt gtactgcaat ggtcggccct
    18601 tctgccagaa tattcaggtt ggcatcggtc accagcgtgg caatctcaat aatgcgatcg
    18661 cgctcgggat ccagaccggt catctcaaga tcgatccaaa tcaggttgtt ttcattggca
    18721 ctcatgctat tttccaccca ttcacagtga ctatattgat ccacaattgc gtgtatcata
    18781 gatgttttgc ccatcagggg cgaccaggag tctgtacgat tgagtaaaaa taaactctcc
    18841 aaaggccagc agcgccgcgt gaacgccaat caccagcgtc gtcttaaaac gtctaaggag
    18901 aagcccgact acgacgacaa tctgtttggc gagcctgatg aaggtatcgt catcagccgc
    18961 tttggtatgc acgctgatgt ggaatccgcc gatggcgacg ttcaccgctg caatattcgc
    19021 cgtaccatcc gttcgttggt gaccggcgac cgcgtagtct ggcgtccggg taaaccagcg
    19081 gcggaaggcg taaatgtcaa agggatcgtg gaagcggtgc atgagcgcac ctcagtgttg
    19141 acgcgcccgg atttttacga cggtgtgaaa cctattgctg ccaacatcga ccagattgtc
    19201 attgtctccg ccattttgcc ggagctgtcg ctcaatatta tcgaccgtta cctggtggcc
    19261 tgcgaaacct tgcagattga gccgattatt gtgctcaaca agatagacct gctggacgac
    19321 gaaggcatgg cattcgtcaa cgagcagatg gatatctacc gcaatatcgg ttatcgcgta
    19381 ttgatggttt ccagtcatac tcaggatggg ctgaaaccgc tggaagaggc gttgaccggg
    19441 cgcatcagca tttttgccgg gcagtctggc gttggcaaat ccagcctgct gaacgcattg
    19501 ctgggtctgc aaaaagagat cctgaccaac gatgtctctg acaactcggg tctcggtcag
    19561 cacaccacga ccgccgctcg gctgtatcac ttcccgcacg gcggtgatgt gattgactcc
    19621 ccaggagtgc gtgagttcgg cctctggcac ctggagccgg aacaaatcac tcagggcttt
    19681 gtcgaattcc atgactactt aggtctgtgt aaatatcgcg attgcaaaca cgataccgat
    19741 ccgggctgcg ctatccggga agcggttgag gaaggaaaaa tcgcggaaac ccgtttcgaa
    19801 aactatcacc gtattctgga aagcatggcg caggtaaaaa cgcgtaaaaa cttttctgat
    19861 acggatgact gacaattaag ctaaccgtcg ttagaatcgt cccctttttt tcaggatccg
    19921 gcatgtaggc cggatcagga acgacaaaac aatggcctgg aggctacctt gttaaattca
    19981 tttaaacttt cgctacagta cattctgccg aaactatggc ttactcgcct ggcgggttgg
    20041 ggcgcaagca agcgggcagg atggctgaca aaactggtta tcgatctgtt cgttaaatac
    20101 tacaaggtcg atatgaaaga ggcgcaaaag ccggacaccg ccagctaccg cacctttaac
    20161 gaattctttg tccgtccgct gcgtgacgaa gtacgcccaa tcgataccga tccgaatgta
    20221 ctggttatgc ctgccgatgg cgttatcagc cagttgggta aaatcgaaga agataaaatc
    20281 ctgcaagcca aaggccacaa ctacagcctc gaagccctgc tggctggcaa ctatctgatg
    20341 gcggacctgt tccgcaacgg tacgtttgtg accacttacc tctccccacg tgactaccac
    20401 cgcgtacaca tgccgtgcaa cggtattctg cgtgagatga tctacgtgcc gggcgatctc
    20461 ttttccgtta accatctcac cgctcagaac gtgccgaatc tgtttgcccg taacgaacgc
    20521 gtgatctgcc tgttcgatac cgaatttggc ccaatggcgc agattctggt cggggcgacg
    20581 attgtaggca gcattgagac ggtctgggcg ggcaccatta cgccgccgcg cgaaggtatc
    20641 atcaagcgct ggacctggcc tgccggggaa aacgacggtt ctgtggcact gctgaaaggc
    20701 caggaaatgg gtcgctttaa actcggttcc accgttatca acctgtttgc accgggtaaa
    20761 gtgaatctgg ttgagcaact ggaaagcctg tctgtcacga aaattggcca gccgctggca
    20821 gtatctaccg aaacctttgt tacaccagac gctgaaccgg ccccgcttcc tgctgaagag
    20881 atcgaagcag aacacgacgc caacccattg gttgacgaca acaaagacca ggtctaatcc
    20941 atcaaaggaa acgctgacgt gcgcctgatt atcacttttc tgatggcctg gtgcctcagt
    21001 tggggggcgt acgccgcgac ggcccccgat agcaaacaaa tctcacaaga actggagcag
    21061 gcaaaagcgg cgaaacccgc gcagccggaa gtcgtagagg cgctccagtc cgccttaaat
    21121 gcgcttgagg aacgaaaagg ttcccttgag cgcatcaagc aatatcaaga agtcattgat
    21181 aattacccga aactttccgc tactctgcgc gcacaattaa acaacatgcg tgacgaaccg
    21241 cgcagcgtgt caccgggaat gtctaccgac gcgctgaatc aggaaattct ccaggtcagc
    21301 agccagttgc tggataaaag ccgtcaggcc cagcaagagc aggaacgcgc ccgcgagatt
    21361 gccgattcgc tgaatcaact gccgcaacag caaaccgatg cccgccgtca gttaaatgaa
    21421 atcgagcgcc gcctggggac gcttaccggc aattccccgc tcaatcaggc acaaaatttc
    21481 gcgttgcagt ctgactccgc acgcctgaaa gcgctcgtcg atgaactgga actggcgcag
    21541 ctgtctgcca ataaccgtca ggaattagcg cgcttacgct cagagctggc ggaaaaagag
    21601 agccagcaac tggatgcgta tttgcaggcc ttacgtaatc agttgaacag ccaacgtcag
    21661 cttgaggcgg agcgggcgct ggaaagtacc gaattactgg cagaaaacag tgccgatttg
    21721 ccgaaagata tcgtcgcgca attcaaaatt aaccgcgaac tatcggcagc gctgaatcaa
    21781 caggcgcagc ggatggatct cgttgcctcg caacagcgtc aggctgccag ccagacgtta
    21841 caggtccggc aggcgttgaa tacgctgcgt gaacagtcgc aatggctggg ctcgtccaac
    21901 ctgctcggcg aagcgttgcg ggcgcaggtg gcacggctac cggaaatgcc aaaaccacaa
    21961 cagcttgata ccgaaatggc gcagttgcgt gtgcaacggt tacgttatga ggatctgcta
    22021 aataaacagc cgctgctacg gcaaatccat caggccgacg gtcagccgct gaccgccgag
    22081 caaaaccgta ttctggaagc acagctacgc actcagcgtg agttgctgaa ctcattgttg
    22141 cagggtggag acacgctact actggaactg accaagctga aagtctccaa cgggcaactg
    22201 gaggatgcgc tgaaagaggt gaatgaagcg acgcaccgct atctgttctg gacctctgac
    22261 gtgcgcccga tgaccatcgc ctggccgctg gaaatcgccc aggatctgcg tcgtctcatt
    22321 tcgctggaca ccttcagtca gttgggcaaa gccagtgtaa tgatgctgac cagtaaagag
    22381 acgattttgc cgctgcttgg cgcgttgatt ctggtcggtt gcagtattta ctcgcgccgc
    22441 tatttcaccc gttttcttga acgttcggcg gcgaaagtcg gcaaagtgac tcaggatcac
    22501 ttctggctga cgttacgcac tcttttctgg tcgattctcg tcgcgtcacc gctgcctgtg
    22561 ctgtggatga cgctgggtta cggcttgcgc gaggcgtggc cttatccgct ggcggtcgcg
    22621 attggtgatg gcgtaacagc caccgtgccg ctgctgtggg tagtgatgat ttgcgccacc
    22681 tttgcccgcc cgaacggctt gtttatcgct cattttggct ggccgcgcga acgggtttcc
    22741 cgtgggatgc gctactacct gatgagcatc gggcttattg tgccgctgat tatggcgctg
    22801 atgatgttcg ataacctcga cgaccgtgaa ttctccggtt cgctgggacg gctttgcttt
    22861 atcctcattt gcggtgcgct ggcggtggtc actctcagtc tgaaaaaggc cgggatcccg
    22921 ctgtatctca acaaagaagg cagcggcgac aacattacca accatatgct gtggaacatg
    22981 atgattggcg caccgctggt tgccattctg gcgtcggcgg tgggttatct ggcaacggcg
    23041 caggcgctgt tagcgaggct tgaaacctcg gttgccatct ggttcctgct actggtggtt
    23101 tatcacgtta tccgccgctg gatgctgatc cagcgccgca ggctggcgtt tgaccgggcg
    23161 aagcatcgcc gggcagagat gttagcgcag cgtgcgcgtg gcgaagagga agcgcatcat
    23221 cacagtagcc cggaaggagc aattgaagtc gatgaaagcg aagtcgatct cgatgccatc
    23281 agtgcgcaat ccttgcggct ggtgcgctca attttgatgt tgatcgccct gctttcggtc
    23341 attgtgctgt ggtcagaaat ccattccgct ttcggcttcc tcgaaaatat ttcgctgtgg
    23401 gatgtcactt ccacggtaca gggcgtagaa agcctggagc cgattaccct cggtgcggtg
    23461 ctgattgcca ttctggtgtt tatcatcacc acgcagctgg tgcgcaacct gcccgcgctg
    23521 ctggaactgg cgattctgca gcacctggat ttaacgccgg gtacgggcta cgccatcacc
    23581 accatcacca aatatctgct gatgctgatt ggcgggctgg tcggcttctc gatgattggt
    23641 attgagtggt cgaaattgca gtggctggtc gccgcgctcg gtgttggtct cggttttggt
    23701 ttgcaggaaa ttttcgccaa ctttatctct ggcctgatta tcctgttcga aaaaccgatt
    23761 cgcattggcg atacggtaac gatccgcgat cttaccggta gtgtgacgaa aattaacacc
    23821 cgcgccacca ccatcagcga ctgggaccgc aaagaaatta tcgtgccgaa caaggcgttt
    23881 attaccgagc agtttatcaa ctggtcgctc tctgactcgg tcacgcgcgt ggtgttgacg
    23941 attccggccc ctgccgatgc caacagtgaa gaagtgacgg aaatcctgct caccgcagcg
    24001 cgtcgctgct cgctggtaat cgataaccca gcaccggaag tcttcctggt ggatctgcaa
    24061 caggggattc aaattttcga gctgcgtatt tacgccgctg agatgggtca ccgtatgcca
    24121 ctacgccatg agattcacca gctaattctg gcaggattcc atgcccacgg tattgatatg
    24181 ccattcccgc ccttccagat gcgtttggaa agcctgaatg gtaagcaaac ggggagaaca
    24241 ttaacttctg cgggcaaagg tcgtcaggcg ggaagtttgt aaggtctttg tggccggatg
    24301 aacccatccg gcttataagc aaaactgata gcgcagaaaa aactgccaca gccaatagcc
    24361 gaaaatagcc agcgcggtgg cgtacataaa ccagccgcgt ttttttcgcc acaaagcgaa
    24421 ccagagaaaa gggaaaaaga gcggtaacgt cattatcaca ccgacatcac gggtatcgtc
    24481 atcttcgaga tacatcagca catcgcagac gttattaatc ccgcgtccat caatcatcca
    24541 ctctttgtcc agcgcggaag tgatggttat ccagaggaac gccacaataa accagatgca
    24601 gcacaagata aacatgcgcg cactcatgag ataatcggtc gcttaatttc gctatagcgc
    24661 atacgaaaca aatcgccgta tcccactaat tctccgcgaa aaaccagaca tctaataaga
    24721 taagcgcaat agatatccgg caggtttccc caccgcggac catcgcgctc tttgagggta
    24781 ttaatagtgt tacccaccac catcgctact ttgcgggtgt ggcgggtatc gacaaaagag
    24841 aggaccgttt gctctatttc cgccacatcc ccggcagaaa attgcagctc cctgatttca
    24901 tcttcggtta tcactggatc ccgactagcg tcgtccataa cgtcttcctt tccagacaac
    24961 tcatcgccgg agagacgatg agttgcagga tttcatatga tcagtcgaga gctaactcag
    25021 gtttgttcgc catgcgactg caatagttct ggtaaatcgc catcgctaac agcgagaaga
    25081 agacagggcc gccaatcatc cacaatgtgc tgtcccagtc tccagcttca accacaggtt
    25141 gaatgatggt gaagacgttc gcaaatgtca ccaccagtac gacaaccact gttgcaatca
    25201 ttgccgacat atgcgttttg aaaatcacaa acggtctgtc gagatcctga cgtgctttaa
    25261 agaatgggaa agccagcgcg aggaacaggt aaggaagcgt catagacacg ttcgccatca
    25321 gcgtcagctt gttaaagaac gccgatgcgg taccgccacc aaacgaaacc agcaggatga
    25381 agacagtaac caacccgcac tgcatccaca tggcgataga cggcatcccc atcgcattca
    25441 ggcgcgtcat cggttccggc cacaacgctt tcggcgtccc ctggatgatg gctttcagcg
    25501 gtgaatagca aagcgtaaag aacgcaccgg tataagcgag gaacatcgaa agtccggtaa
    25561 tacgcgcaaa ccatacgccc agcgacaatg acgcttcagg tgacaaatgc agtgcgttac
    25621 ccagcgtcac cccgaggctc ttcatcagca cataggtaat attgccgagg ttaacggaac
    25681 cattacttaa tacctgctgc cagtttgtgc tgacgcccca taaaaatatt gccagcgaat
    25741 aaccgattga aataacaata gcggcaaaaa caataccttt ggcaaagttc ttttctggat
    25801 tttccgtttt atcgaccaga ccaccaaccg cttcaattcc accataggca aaaatagcaa
    25861 ataccacaaa cgatagcatt gccagaccgg actgataacc tgggttcggt gatgcaagga
    25921 aattaatatc ctgcgcgaaa tgcccaccat ttaataacaa aatagtaatg cttactaaca
    25981 gcaatactaa attcagacac attactgcaa taccgcccac cgcagtaatg cgggcaattt
    26041 tattaatccc ttttgaagca acgacggtga ccagaatcat ccatgccact gccagcagac
    26101 caaccacctg cgtaggctcc agtccggcaa tacgccagtg ctgggtcatg tcgctaccat
    26161 agaggaatgt tgagaacggt acccaaactt tcgcggaggt actcaccatc cagatgatat
    26221 aagaggaaaa ccacataaac gtaccaatga aggcaaaacg tggtccgaca ctattattca
    26281 tccaggaata gataccgcct tcttctttgc gataagcagc tcccatttca gccatcatta
    26341 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
    26401 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
    26461 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaaaca tccttcattt
    26521 cagcgcgtgt ataaaacgcg cccactgaac tgcactgccc aacattgagc gggcagcgta
    26581 aaaaccaata cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct
    26641 ttagcggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgcggt
    26701 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
    26761 tgtctccgcg cccagcgcca acatcaccag acgatcaacg cctaatgcca cgccggaaca
    26821 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
    26881 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
    26941 cgtcaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
    27001 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
    27061 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
    27121 ttgtagaagc gtgtcgcggt cttcttcagt atcggcaacg ttgctcaaat ccagtttcgc
    27181 tgccacttcc cgcagttgcg ttttgtcggc agagagtggg tcaatttcca gataacgcaa
    27241 gaaagcttgt tgataagaaa ggctttctgc tgccggacag tccagcacct gttgcaagag
    27301 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
    27361 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
    27421 gaataccggc ccacaaccag caaccagcag gcgtttcata tggtattccg ggctggtcat
    27481 taaccagaga ttcatcccct gcgaatgccc ggggcctacg aaacgtgtct caaacgggac
    27541 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
    27601 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg
    27661 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
    27721 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
    27781 aagggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
    27841 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
    27901 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
    27961 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
    28021 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
    28081 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
    28141 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
    28201 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
    28261 caggatgtcg tggattattt cgtccaccac tgccccaccg aaatgaccca actggaactg
    28321 tggggatgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
    28381 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
    28441 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtactg
    28501 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
    28561 acgctggtgc agatccgtgc taacgcggtc gttatggcta ccggcggtgc gggtcgcgtt
    28621 tatcgttaca ataccaatgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
    28681 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
    28741 tccggtatcc tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat
    28801 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
    28861 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
    28921 cgtaaaggca acactatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
    28981 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
    29041 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
    29101 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
    29161 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gttctaactc cctggcggaa
    29221 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
    29281 aatggcaacg aagcggtaat tgaagcgcag gcagctggcg ttgaacaacg tttgaaagat
    29341 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggct
    29401 atggaagaag gctgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
    29461 ctggcagagc tgcaggaacg cttcaaacgc gtgcgcatca ccgacacttc cagcgtgttc
    29521 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
    29581 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
    29641 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
    29701 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
    29761 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
    29821 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
    29881 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
    29941 tgcgctgggc tacatcaaag acaacctggc accggacctg agctaccgct ggtcctgccg
    30001 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
    30061 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
    30121 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
    30181 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
    30241 gatggccaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
    30301 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
    30361 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
    30421 gaacggcgta tggagctgta ctttcgtggg ctactgctcc gaagtctgcc cgaaacacgt
    30481 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
    30541 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
    30601 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttctacatg ctgcgcgaag
    30661 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
    30721 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
    30781 tcattaacct gatcactctg gcggcagccc tgctgcacac caaaacctgg tttgagctgg
    30841 caccaaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
    30901 aaagcctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
    30961 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
    31021 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
    31081 gttggtattc tgctgccact ggggctgttt ccgggtgacg cactgagcta cgagcgcgtt
    31141 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
    31201 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtacct
    31261 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
    31321 gtcgttacaa tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg
    31381 tatggaaacc agaccctatg ttcaaaatga cgctctgcgc cttattaatt accgcctctt
    31441 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
    31501 ttatagagca acaaaagatc cccggtatag cggtggcggt aatttatcag ggtaaacctt
    31561 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
    31621 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
    31681 ctcgagggga aatcaagtta agcgatcccg cgacaaaata ctggcctgaa cttaccgcta
    31741 aacaatggaa tgggatcaca ctattacatc tcgcgaccta caccgctggc ggcctgccat
    31801 tgcaggtgcc ggatgacgtt aaatcctcaa gcgacttgct gcgcttctat caaaactggc
    31861 aacctgcatg ggctccagga acacaacgtc tgtatgccaa cgccagtatt gggttgttcg
    31921 gtgcactggc ggtgaaacca tccggtttga gctttgaaca ggcgatgcaa actcgtgttt
    31981 tccagccact caaactcact catacgtgga ttaatgtgcc gtccgcagaa gaaaagaatt
    32041 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
    32101 aggcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
    32161 aaccccttga tatcactgag aaaacgcttc aacaagggat acaactggca caatctcgct
    32221 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
    32281 atcctgacat catcattaac ggcagtgaca ataaaattgc actggcagca cgccccgtaa
    32341 aagcgattac gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
    32401 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
    32461 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
    32521 ctctacagta aaatttcatc gggtccgaat ttttggacct tttctccgct tttccttgct
    32581 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
    32641 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccccgtggc
    32701 gtgaccgtag tgaataattt cgatgctaaa cgctatctcg gtacctggta tgagattgcc
    32761 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt
    32821 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagaga aatgtggcag
    32881 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
    32941 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
    33001 gcgctggttt gcggtccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
    33061 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
    33121 aagtttattt gggtacagca gcccggtagt tagttagtga gtgctgagtt tcagaccaat
    33181 aatccccaat acgattaacg ccagactcgc caggcgcatc gggttagcgg actcgccgag
    33241 cagcacaatg ccggtgatgg ccgcgccgac tgcgccaata cccgtccaca cggcataagc
    33301 cgtccctact ggtaacgatt tcatcgccca ggcaagtagc gccatactga caatcatcgc
    33361 cgtcacagta ataacacttg gcgtcaaacg actaaagccg tgggtatatt tcaggccaac
    33421 ggcccatacc acttccagca ggccagcaat aactaagata atccaggaca tatcaggctc
    33481 cggagaattg gggccgtccc cggtgaaaag aggcgtttgc aggtcgtcct gcaaagctga
    33541 tggttgaacg gatattttgc caggtggcag gggaatttca actattacct ttgtgactat
    33601 cgcaaaaaaa caaacggcac gacacagctg tcgtaccgtt attgcttact gctgcgcttt
    33661 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
    33721 ggtgttgcaa gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
    33781 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
    33841 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga ggagaagaaa
    33901 aattagctgg cagcgcggga gatggagttg ccgagatgtt taatgtcttc accaaaaccg
    33961 cgagcggtgt tacagcccgt gagcagcgca ctggcaagca aaacaagaac gataaggcgt
    34021 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
    34081 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatc
    34141 tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagctgg tttgccgcca
    34201 gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag ctcaacgaag
    34261 ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga
    34321 tccagcaacc atttggcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa
    34381 gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata
    34441 tcgacaacat cagcaccttc agcggaatca gtagatttga aggttttttc tacgcgagta
    34501 ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg
    34561 aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga
    34621 aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag
    34681 ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca
    34741 acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga
    34801 cgaaaaactg ttggccgggc gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc
    34861 atttatcgat cgcatggaga aaggcaatcc gaacgatccg cttttgcgtc aggtacttac
    34921 ctcgcaagat gagtttgttg tcgcgcccgg attctccacc gacccgctgg aagaacagca
    34981 cagcgtagtg ccaggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg
    35041 cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca
    35101 gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga
    35161 cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt
    35221 gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc
    35281 gattgtgatc ccggcacgca tcacagatgc gctggttgaa cgcttttctc attctacgct
    35341 gcaaatcttg ctggtgaatc acattaacca tgccaatgag gtagatgaaa cattccgtca
    35401 ggcgatggct aagttgcgcc gggtaggcgt tactttgctc aaccagagcg ttctgttacg
    35461 tggtgtgaac gataacgcgc aaacgctggc aaacctgagt aatgcgttgt tcgatgcggg
    35521 cgtgatgccg tattacctgc atgtgctcga taaagtacag ggcgcggcgc attttatggt
    35581 gagtgatgac gaagcacggc agattatgcg tgagttgctg acactggtgt cgggatatct
    35641 ggtgcctaaa ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc tggatctcca
    35701 gctacgccag cagtaactga ttaaacaggg aagtttaatc tttgcacgtt gcat
//
LOCUS       DAZGAP010000022        34757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGAP010000022 DAZGAP010000000
VERSION     DAZGAP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.022"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(98..1084)
                     /gene="yeiR"
                     /locus_tag="ACHLAM_000957"
     CDS             complement(98..1084)
                     /gene="yeiR"
                     /locus_tag="ACHLAM_000957"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO7852524.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1202..2668)
                     /locus_tag="ACHLAM_000958"
     CDS             complement(1202..2668)
                     /locus_tag="ACHLAM_000958"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO7852525.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARALMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNTIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            complement(2891..3463)
                     /gene="yeiP"
                     /locus_tag="ACHLAM_000959"
     CDS             complement(2891..3463)
                     /gene="yeiP"
                     /locus_tag="ACHLAM_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO7852526.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            3618..3872
                     /locus_tag="ACHLAM_000960"
     CDS             3618..3872
                     /locus_tag="ACHLAM_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7852527.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(3869..5050)
                     /gene="setB"
                     /locus_tag="ACHLAM_000961"
     CDS             complement(3869..5050)
                     /gene="setB"
                     /locus_tag="ACHLAM_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO7852528.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVTVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            5418..6548
                     /gene="fruB"
                     /locus_tag="ACHLAM_000962"
     CDS             5418..6548
                     /gene="fruB"
                     /locus_tag="ACHLAM_000962"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO7852529.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            6548..7486
                     /gene="fruK"
                     /locus_tag="ACHLAM_000963"
     CDS             6548..7486
                     /gene="fruK"
                     /locus_tag="ACHLAM_000963"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO7852530.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFDVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMCESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            7503..9194
                     /gene="fruA"
                     /locus_tag="ACHLAM_000964"
     CDS             7503..9194
                     /gene="fruA"
                     /locus_tag="ACHLAM_000964"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO7852531.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVATSG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYISFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            9618..10559
                     /locus_tag="ACHLAM_000965"
     CDS             9618..10559
                     /locus_tag="ACHLAM_000965"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO7852532.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            10547..11479
                     /gene="psuG"
                     /locus_tag="ACHLAM_000966"
     CDS             10547..11479
                     /gene="psuG"
                     /locus_tag="ACHLAM_000966"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO7852533.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAQGVIGKESTPFLLARVA
                     ELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            11573..12823
                     /locus_tag="ACHLAM_000967"
     CDS             11573..12823
                     /locus_tag="ACHLAM_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7852534.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12891..13589)
                     /locus_tag="ACHLAM_000968"
     CDS             complement(12891..13589)
                     /locus_tag="ACHLAM_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO7852535.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            13719..14660
                     /gene="rihB"
                     /locus_tag="ACHLAM_000969"
     CDS             13719..14660
                     /gene="rihB"
                     /locus_tag="ACHLAM_000969"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO7852536.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            14760..16010
                     /locus_tag="ACHLAM_000970"
     CDS             14760..16010
                     /locus_tag="ACHLAM_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7852537.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKSFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16066..17154)
                     /locus_tag="ACHLAM_000971"
     CDS             complement(16066..17154)
                     /locus_tag="ACHLAM_000971"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7852538.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17157..18014)
                     /gene="nfo"
                     /locus_tag="ACHLAM_000972"
     CDS             complement(17157..18014)
                     /gene="nfo"
                     /locus_tag="ACHLAM_000972"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO7852539.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18088..19137)
                     /locus_tag="ACHLAM_000973"
     CDS             complement(18088..19137)
                     /locus_tag="ACHLAM_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO7852540.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19236..20117
                     /gene="yieE"
                     /locus_tag="ACHLAM_000974"
     CDS             19236..20117
                     /gene="yieE"
                     /locus_tag="ACHLAM_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO7852541.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20322..21791
                     /gene="lysP"
                     /locus_tag="ACHLAM_000975"
     CDS             20322..21791
                     /gene="lysP"
                     /locus_tag="ACHLAM_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO7852542.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAVS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22084..24063
                     /gene="cirA"
                     /locus_tag="ACHLAM_000976"
                     /pseudo
     CDS             22084..24063
                     /gene="cirA"
                     /locus_tag="ACHLAM_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(24095..24931)
                     /gene="yeiG"
                     /locus_tag="ACHLAM_000977"
     CDS             complement(24095..24931)
                     /gene="yeiG"
                     /locus_tag="ACHLAM_000977"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7852543.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25189..25857
                     /gene="folE"
                     /locus_tag="ACHLAM_000978"
     CDS             25189..25857
                     /gene="folE"
                     /locus_tag="ACHLAM_000978"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO7852544.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            25874..27031
                     /gene="yeiB"
                     /locus_tag="ACHLAM_000979"
     CDS             25874..27031
                     /gene="yeiB"
                     /locus_tag="ACHLAM_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO7852545.1"
                     /translation="MGRNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPARSKTSR"
     gene            27173..28213
                     /gene="galS"
                     /locus_tag="ACHLAM_000980"
     CDS             27173..28213
                     /gene="galS"
                     /locus_tag="ACHLAM_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO7852546.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            28493..29491
                     /gene="mglB"
                     /locus_tag="ACHLAM_000981"
     CDS             28493..29491
                     /gene="mglB"
                     /locus_tag="ACHLAM_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO7852547.1"
                     /translation="MNKKVLTLFAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            29552..31072
                     /gene="mglA"
                     /locus_tag="ACHLAM_000982"
     CDS             29552..31072
                     /gene="mglA"
                     /locus_tag="ACHLAM_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO7852548.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31088..32098
                     /gene="mglC"
                     /locus_tag="ACHLAM_000983"
     CDS             31088..32098
                     /gene="mglC"
                     /locus_tag="ACHLAM_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO7852549.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(32169..33404)
                     /gene="preA"
                     /locus_tag="ACHLAM_000984"
     CDS             complement(32169..33404)
                     /gene="preA"
                     /locus_tag="ACHLAM_000984"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO7852550.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(33398..34636)
                     /gene="preT"
                     /locus_tag="ACHLAM_000985"
     CDS             complement(33398..34636)
                     /gene="preT"
                     /locus_tag="ACHLAM_000985"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO7852551.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLKGACSC"
BASE COUNT         8399 a         8558 c         9156 g         8644 t
ORIGIN      
        1 agaaacagcg cgaggccgac aatattcaac aacactattt gcggcaaatt tttaatcata
       61 ttttctctaa ttatcttact gaaaaacgca ggcaacctta cgcggtggtc gctaaacgaa
      121 gcttcaacag cgcgctctgc aaggcattcc agtcagcttc gctgctggaa atcagctcaa
      181 tacgactgtc cggtggcgca acgttttgcg tttcaatgtg caggtcatcg ccctgacggt
      241 tgattcgcac cagcccttcg ggaatacgca tcacgccttt gacgcgttcc accggtgcaa
      301 gtcgcgccca ttccagaatg ccaatggtgt cgaataccgt atcagcgtcg aatatccagc
      361 cacaggcctg atatccttgc ccgctgttca gactgcgacg ccagcgttga tgctctggca
      421 ggcttaacgc tgctaaccct tttttcacgc catgctgatg agaatgcgcg gcgctggcgg
      481 gcaactcggc taaattgcga cgcggcaaat ccagaagatg accgtcaatt ttcccatgct
      541 cgctgtgaat taattgccga tcgccaccat tttgctgcca ccagcgctgt agcgcttgct
      601 cactttcggg cgtcgcacgg tcggatttat tggcgacaat aatgtctgcg gcagccagct
      661 ggtcacggaa gttttcattg ctggcgcttt tttcgtccag cagcaggcgt ggatcgagaa
      721 tgcacaacgt ggcgcgcaga tctatccacg gttcatacac gggcgcggtt aagagatcta
      781 ggatctgttt cggatgaccc agtccggtcg gctctatcaa caagcggtct ggttttccct
      841 gacgcagtaa ggtattcaat cctacctgca tgggtaaacc attaacgcag cacatgcagc
      901 cgccggggat ctctttcagc aatgcgccgc tatcggcgag caaagcacca tcaattccga
      961 cttccccaaa ttcattaacc agtaccgccc atttttcgtt gggatcttta tgggctaaca
     1021 gatgaagaat cgacgtggtt ttcccgctgc cgagaaaacc ggtgatgagg ttggtcctgg
     1081 tcacaacggc tccgcaatac tcttaaaagt taactgagcg ttaatcctgg ctaaaaacgg
     1141 cagaaaagag aaggggagag cggacgaagg cgagcttcat ccgctttaac cgcagaaatc
     1201 gttaaatttt gagggtgttt aatagcgcct gatgcgcgcc gaattgtgcg atttgttgcc
     1261 aggcttgttc gatggcctgc acaaaatgcg ggttatctgg cagatcatca ccgaaaactt
     1321 cacgcaggga aagcagggcg gtgacgcgtt gctcactgct gctgcccgca acaagatcgc
     1381 gaattttatc gctaagcgga tcgcgaacat caatggtatt tccggcatca tcaacgccgc
     1441 tgacgtaacg catccagcct gcaacgccta atgccagcaa cgaccagtcc gtttcgcgcg
     1501 ccagatgtat gcgaatacct gccagcatac gttgcggtaa tttctggcta ccatccattg
     1561 cgatttgcca ggtcttatgt ttcagcgccg ggttagcaaa acgtgcaatt aacttatccg
     1621 catattgtgt taaatcgaca tctttaattc gcagtgtcgg cgcttgctca tccagcatta
     1681 atgctctggc ggcatggcga aatgcgcgat cctgcatgca atcactgata tgggcgaatc
     1741 ctgagaggta acccagataa gcgagaaaag agtggctgcc attaagcata cgcagtttca
     1801 tctcttccca tggcaggaca tcattcacca tttgtacacc tgcgacttcc caggcaggac
     1861 gcccagcgac gaagttatct tccaccaccc actggataaa cggttcgcag ctaatcgcgc
     1921 agggatcatt tacccccaga tgctggctga tttccgccag tgattcgtcg gttgctgccg
     1981 gaacaatgcg gtcgaccata gttcccggaa aactgacgtg ctcttttatc cacccggcga
     2041 gttctggcga acgtttttct gccattccca gcaccgcgtt tttcaccaca tggccattgt
     2101 cgggaatatt gtcgcaggag agcacggtaa acggtgtaag gccgcgctca cggcggcgtt
     2161 tcagtgcttc gacgagaata cccggtgcgg agtgaggttc ttcaggggtt tgtagatcgt
     2221 gaataatccg cggattgctg gtgtcgagtg caccggtggc cgggtcaata caatagcctt
     2281 tttcggtaat cgtcagggaa acaattgcca cctgtggctc gcaaaatttc tcaataattg
     2341 ccgctaagga atccagtttt gcattcaggc attcgtgaac ggcaccgaca attatcgcct
     2401 gattgccgtc cgcccctttc tccagcacgg tgtataaatg gttctgtgcg cggagctggc
     2461 tcatcagttg atcaccgctg aacaagctga tttcacagat cccccagtcg ccgctctggg
     2521 cattcagcac acgatcggtc agtaacgcct gatgtgcgcg gtgaaaggct ccaaagccaa
     2581 aatgaacgat acgtgattgc aactgctggc gatcgtagcg tggagcgtgt acatgatgcg
     2641 ggagcgtaac ggaggcgatt gtattcatag gagtcacgtc ttagtaacga ttaattaaca
     2701 atgacagtgt aaagttatat gacagcaatt gaaattggtc tggattgtta atgcgatgaa
     2761 gtgtgagggc gatcaatcaa cgagtgaaaa ttgcgggcgt aaggtgttca ggaagcgcaa
     2821 aatagaagat tggtataata aattaaaaaa gggacagcct gagctatccc ttttctgcat
     2881 gcggctgaag ttagtcagca cgccccatat aacggcgttc ttcgatatgg atacgaattt
     2941 tttcgcccgg gcttaagtat tctggtacct gaatcaccag accagtgctc aatgtcgccg
     3001 gtttgttacg ggcgctggca gatgcccctt tgatgcctgg tgcggtttca acgatttcca
     3061 gatcaacggt ctgcggcaac tcaagcgcca gcagttgacc gtcccaggtc agcacctgca
     3121 tgtccggcat gccgccctct ggcataaaca gcaactcttc ttcaatctga tctttggtga
     3181 aggtatacgg ggtatagtct tctttatcca taaagacata ttcgttgcca tcgacatagg
     3241 agaagtcaac gtaacggcgg gtcagcgtca cggtgtcaac gatatcatct cctttgaaac
     3301 gctcttctac tttcagcccg gtacggacat cagaaaaacg cattttgtac agcgtagcgg
     3361 caccgcgggc agtgggcgac tgaatatcaa tgtccttaac cagcagcagt ttgccgttgt
     3421 aattcagtac catacctttt ttaatttcgt tcgctcttgg catcgaaaaa ttcctgtagt
     3481 cgaaaatgtc aaaaatatcg cgacaaagta ctcgcgacgg gcctttcggg caagtggtat
     3541 tcgcactttt gctggtgcaa aaaaggtggt actgtgcgcg ctcatcaatc cggtggttaa
     3601 ccttaagaga acaacgtatg gaatgccgtc cgggttgcgg ggcgtgttgc actgcccctt
     3661 cgatttccag ccctattccc ggtatgccag atggcaagcc cgccaatacg ccctgcattc
     3721 agcttgatga acagcaacgc tgtaaaattt tcacctcgcc actgcggccg aaagtctgtg
     3781 caggcttaca agccagcgct gaaatgtgcg gtaactcgcg tcagcaggca atgacctggc
     3841 ttatcgatct ggaaatgctg accgcgcctt aaacatcttt aatccgcagt aagcaaaaca
     3901 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
     3961 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgta ttggtataga
     4021 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
     4081 cacccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atgacaggtg
     4141 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
     4201 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatggtc gggatttcca
     4261 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
     4321 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
     4381 tgacaaacag cagcagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
     4441 ccagcggaag ttctttttgc attgacggta aaaacagcca caccgtcacg ccgcaaacaa
     4501 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaaggcat
     4561 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
     4621 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
     4681 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
     4741 tgcgattcca ggcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
     4801 gcgatttgcg atcgccgcgc ttatcagagc gcccggcgag aaactgactc accagaatcc
     4861 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     4921 cggtaagaaa aatactgagt gtcggggttt gcagagcgcc cgcaataccg gtgagaaagg
     4981 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggtg actgcggggg
     5041 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     5101 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     5161 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     5221 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     5281 tttccttgcg tgctgaaaca aaagtattat gctctcttga aacgtttcag cgcgatcttg
     5341 tctttaaccc taagccaggt tggcgctttt tttctcatag aagctgaatc gtttcaattc
     5401 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     5461 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     5521 tagctgaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     5581 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     5641 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggccag gtagcgtacg
     5701 tggcgatcgg tattgctgcc agctcggatg aacatctggg cctgctacgc cagctgaccc
     5761 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     5821 ttcgcgcatt gctgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     5881 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     5941 tgaaagaagc gggggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     6001 tgaacctcgg gcagggtatc tggctgagcg atagcgccga aggcaatctg cgtagcgcga
     6061 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     6121 tgagtgtggc gatgaatgac gatcagccca ttgcggttct taagcgtctc gctgatttgt
     6181 tactcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     6241 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     6301 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     6361 gtgatattac cgtgacaaac cttgatggta ccggcaaacc ggcaaacgga cgtagtctga
     6421 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     6481 cagatgctga acaggcgctg aaagcaatcg gcgacgctat cgctgctggt cttggggagg
     6541 gcgcataatg agcagacgtg ttgctactat cacccttaat ccggcttatg accttgttgg
     6601 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     6661 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     6721 tggtggcttc ctcggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     6781 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     6841 aaaagacggc gaagtgaccg acttcaactt ctcgggtttt gacgttaccc ccgccgactg
     6901 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     6961 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     7021 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     7081 agcggcaccg tggctggtga aacctaaccg ccgcgagctg gaaatctggg caggccgtaa
     7141 actgcctgaa atgaaagatg tgattgaagc tgcgcatgcg ctgcgtgaac aaggtatcgc
     7201 gcatgtcgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     7261 gatcgccaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     7321 tggtggcctg atttatggct tgctgatgtg tgaatccagt gaacacacac tgcgtctggc
     7381 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     7441 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     7501 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     7561 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     7621 acgctgaaat ggcgattgtt ctcggtgatt ccatcccgaa cgacagcgcg ctgaacggta
     7681 aaaatgtctg gctgggcgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     7741 aagccaaagg ccatgcgaaa ccttacactg caccggtcgc tgcgacagca ccagttgcca
     7801 ccagcggtcc gaaacgcgta gttgcggtga cagcttgccc gactggcgta gcacacacat
     7861 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     7921 aaacccgtgg ttctgttggc gcgggtaatg cgatcacccc tgaagaagta gccgcagcgg
     7981 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     8041 tgtatcgcac ctctaccggt ctggcgctga agaaaaccgc gcaggaactg gataaagcgg
     8101 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacagcgacc actgaaggta
     8161 agaaagaggg tgcaggcgct taccgtcact tgctgacggg cgtctcttac atgctgccga
     8221 tggtcgttgc aggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     8281 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     8341 tgatggtgcc ggtactggca ggttacattt ccttctccat tgccgatcgt ccgggtctca
     8401 cgccgggtct gattgggggt atgctggcgg tcagcaccgg ttctggcttc attgggggta
     8461 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     8521 cacagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     8581 tcggtctggc gatgatctac ctgatcggta aaccggttgc tggcattctc gaagggttga
     8641 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     8701 gcatgatgtg tactgacatg ggcggtccgg taaataaagc agcgtacgca ttcggtgtgg
     8761 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     8821 caccgctggc aatgggtctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     8881 aggaaggtgg caaagccgct ctggttcttg gtctgtgctt tatttcggaa ggtgcaattc
     8941 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgttg tatcgttggt ggggcgctga
     9001 ctggcgcaat ctcaatggcg attggtgcga aacttatggc accgcacggt ggtctgtttg
     9061 ttctgctgat ccctggcgct attacgccgg tactgggtta cctggtagca attattgccg
     9121 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     9181 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     9241 atgagcgcaa agggaatgac cagtttgtga atgtaatttt atgaatttaa atggttttat
     9301 ctttcagtag aaacctgaaa tgttcatcaa acactaaggt tatttcctca agggcagatt
     9361 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcactttt
     9421 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     9481 tggcggcaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     9541 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacaggat tggtaatttt
     9601 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     9661 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     9721 taaagtttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggga
     9781 ataaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     9841 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     9901 cgagctattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     9961 atattagcaa cgctattaca gctgaatatc tcgcacagca cggtgaattt attcagaggg
    10021 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
    10081 atgccgccaa cgtacccgta tttgtcgatc ctgtttccgc gtggaaatgt gtcaaagtgc
    10141 gcgaccgtct aaatcaaatc cacactctca agccaaaccg ccttgaagcg gaaaccctga
    10201 gtgggattgc tttgtcaggg cgtgaagatg tggcaaaagt tgctgcctgg ttccatcaac
    10261 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agtgatatca
    10321 gcggtgaaag tggttggtct gcgccgatca aaaccaatgt cattaatgtt accggagcgg
    10381 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
    10441 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
    10501 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
    10561 aatttcccct gaattattac aaatttcccc ggaagtgcag gacgctttaa aaaacaaaaa
    10621 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
    10681 ccagaccgca attgaagttg aagaaactat tcgtaaacag ggcgctgtac ctgccactat
    10741 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaagaaattg aattgctggg
    10801 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
    10861 cggaaaaaat ggtgcaacca ccgttgcttc aacgatgatt attgcggcgc ttgccggaat
    10921 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
    10981 tatttctgcc gatttgcaag aactggcgca caccaatgtc accgtcgttt gtgccggggc
    11041 gaaatctatt ctcgatttag gcttaactac cgaatattta gaaactttcg gtgtaccgct
    11101 aattggctat cagaccaaag cgctgcctgc gtttttctgc cgcaccagcc cgtttgacgt
    11161 gagcattcgt ctcgacagcg ccagcgaaat tgcccgtgca atggcggtga aatggcaaag
    11221 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg ctatgccgga
    11281 acacactatc aatgcggcga tcgatcaggc ggtagctgaa gcgcagggtg ttattggtaa
    11341 agaaagtacg ccattcctgc tggcgcgcgt tgctgagttg accggcggtg acagcctgaa
    11401 atccaacatc cagctggtgt tcaacaacgc catcctggcg agtgaaatag ccaaagaata
    11461 ccagcgtctc gcgggttaat ttctgacggg cagaaagccg tcgcccgtac tcatcaagca
    11521 taccaaaacc tggcgctgac gccgggttta ctggcatgaa gggaaaatca ttatggatat
    11581 aatgagaagt gttgtgggga tggtggtgtt actggcaata gcatttctgt tgtcagtgaa
    11641 taaaaagagc atcagtttgc gcacggttgg agccgcactg ctgctgcaaa tcgctattgg
    11701 tggcatcatg ctctacttcc caccgggaaa atgggcagta gaacaggcgg cattaggcgt
    11761 tcataaagtg atgtcttaca gtgatgccgg tagcgccttc atttttggtt cgctggtcgg
    11821 gccgaaaatg gatgtcctct ttgacggtgc gggttttatc ttcgcctttc gcgtacttcc
    11881 ggcgattatc ttcgtcactg cgctcatcag tctgctgtac tacattggcg tgatggggct
    11941 gctgattcgc atccttggca gcattttcca gaaagccctc aacatcagca aaatcgaatc
    12001 ttttgttgcg gttaccacta ttttcctcgg gcaaaatgag atcccggcga tcgttaaacc
    12061 gtttatcgat cgcatgaatc gcaacgagtt gtttaccgca atttgtagcg ggatggcgtc
    12121 cattgctggt tcgatgatga ttggttatgc cggaatgggc gtaccaattg actacctgtt
    12181 agcggcatcg ctgatggcga tccctggtgg tattttgttt gcacgtattc ttagcccagc
    12241 caccgagcct tcgcaggtga catttgaaaa tctgtcgttc agcgaaacgc cgccaaaaag
    12301 ctttatcgaa gcggcggcga gcggtgcgat gaccgggctg aaaatcgccg ctggtgtggc
    12361 gacggttgta atggcgtttg tcgcaattat tgcgctgatc aacggcatta tcggcggaat
    12421 tggcggctgg tttggtttcg ccaatgcctc tctggaaagt atttttggct atgtgctggc
    12481 accgctggcg tggatcatgg gtgtggactg gagtgatgcc aatcttgcgg gtagcctgat
    12541 tgggcagaaa ctggcgatta acgaattcgt cgcttacctg agtttctccc catacctgca
    12601 aacgggcggc acgctggaag tgaaaaccat tgcgattatc tcctttgcgc tttgtggttt
    12661 tgctaacttt ggttctatcg gtgttgtcgt tggggcattt tcggctattt cgccaaaacg
    12721 cgcaccggaa atcgcccagc ttggtttacg ggcgctggca gcagcaacgc tttccaacct
    12781 gatgagcgcg accattgccg ggttctttat tggtttagct tgagtgtcat tgccggatgt
    12841 acgcgcttat taagcgtcac atccggcaat cgctctattg caaataattt ttactgcatc
    12901 atccctgaga atttattttc cgggtccatc tccagtgcca gtcctgacaa ctgctttctg
    12961 tttttaatga gatacccttt cttgctttta attaataaac cgtcgtgaat gaattgcgcg
    13021 agaacatata aaagatgtcg ataagaaaca cctaaatact ctgccgcttg cgtatgcttt
    13081 tcgtgataaa gatcaccttc ctgcgagagt aatataaaag ctgccaggcg attcactaac
    13141 ggaaatgatt gattctgagt taaagaaaca atattacgat aatttttatg acttaaggtg
    13201 acgcagagtt ttcgtaaaaa tagcgtgtcg tttaataaca gcgggcggta atgtttcata
    13261 gggagcgcaa ggcaccaaca ctcttcaata gcctgcaccg cacgcggttc atggtctttg
    13321 tcgattaact caatctcgcc aataaaacag ggagcggcaa agaaatcgat cagcgacacg
    13381 cgaccattag caagcgtggc gtaaagcctg gcacggcctc gcgtcagata aaacagccat
    13441 gacggctgtt gcccttcctg cacaatgtag tcacgcgcta aaaaatgaaa cagccgcgta
    13501 tcggctgaaa catccgttga aaagcaatcc ttaaacgcag attcactcat caattgctgc
    13561 ttaaggtcat tattgtggat ttctttcacg atgtttatcc gctatgagat ttctcatatt
    13621 tagcatactt acgctgttgt taagattcaa tcatcggccc ggtcgaattt tctttgctgt
    13681 agaaaaaata acgcaactgg aaacagagga aataaacaat ggaaaagaga aaaattattc
    13741 tggattgtga tccgggtcat gatgatgcta ttgccataat gatggcagcg aaacatccgg
    13801 cgattgattt attaggtatc accattgtgg cgggtaatca gacgcttgat aaaacattaa
    13861 ttaatggcct gaatgtttgc cagaaactgg agattaatgt tccggtttat gcggggatgc
    13921 cacagcccat tatgcgtcaa caaatcgttg ccgataatat tcacggtgaa accggactgg
    13981 atggcccggt attcgagccg ctgacccgcc aggcagaaag cactcatgcg gtgaaatata
    14041 tcattgatac cctgatggca agcgatggcg atatcacttt ggtgccggtt ggcccacttt
    14101 caaatatcgc ggtggcaatg cgtatgcaac ccgcgatcct gccgaaaatc cgtgaaattg
    14161 tgctgatggg cggcgcttac ggtactggca acttcacccc atctgccgag ttcaacatct
    14221 ttgctgaccc ggaagccgca cgcgtggtgt tcacctccgg cgttccatta gtgatgatgg
    14281 gcctcgatct caccaaccag accgtttgca ccccggacgt gattgctcgg atggaaaggg
    14341 caggcggccc tgccggagag ctgttcagcg acatcatgaa cttcactctc aaaacccagt
    14401 tcgaaaacta cggcctggct ggcggcccgg tgcatgacgc cacctgcatc ggttatctga
    14461 ttaaccctga tggcattaaa acccaggaga tgtacgtcga agtggacgtt aacagcggcc
    14521 cttgctacgg gcgtacggtc tgcgacgagc tgggcgttct tggcaagccc gccaatacca
    14581 aagtcggcat cactattgat actgactggt tctggggatt agtcgaagag tgcgtgcgcg
    14641 gctacatcaa aacccattaa gtgttgataa caagccggga cagtatcgca agatgcaggc
    14701 ccggctattt ctgatgggca gaaagccgtc gcccaaaatg acatgaagag ataataacta
    14761 tggatgtcat gagaagtgtt ctgggaatgg tggtattgct ggcaattgcg tttttgctgt
    14821 cagtaaacaa gaagaagatc agcctgcgta ccgttggcgc ggcgttagtg ttacaggtcg
    14881 tgattggcgg cattatgctt tggttaccac cagggcgttg ggtcgcagaa aaagtcgctt
    14941 ttggcgtgca taaagtgatg gcgtacagcg atgcaggtag cgcatttatc tttggttctc
    15001 tggtcggacc aaaaatggat accttatttg atggcgcagg atttatcttt ggtttcaggg
    15061 tattaccggc aattatcttc gttacagcac tggtgagtat tctctactac atcggtgtga
    15121 tggggatctt aattcgcatt ctcggcggta tcttccagaa agcattgaat atcagcaaga
    15181 tcgagtcatt cgtcgcggtc actaccattt tcctcgggca aaacgaaatt ccggcaatcg
    15241 tcaaatcctt tatcaatcgt ctgaatcgca atgaattatt tacagcgatt tgtagcggga
    15301 tggcctcgat tgctggttcg acaatgattg gttacgccgc actgggcgtg cctgtggaat
    15361 atctgctggc ggcatcgtta atggcgatcc ccggcgggat cttgtttgcc cgcctgttaa
    15421 gcccggcaac agaatcttcg caggtttcct ttaataacct ctctttcacc gaaacaccgc
    15481 caaaaagcat tattgaagcc gctgcgacag gggcaatgac cgggctgaaa atcgccgcag
    15541 gtgtggcgac agtagtgatg gcatttgtcg ccatcattgc gttaattaac ggtattatcg
    15601 gcggcgttgg cggctggttt ggctttgaac atgcctcgct ggagtccatt ttaggttacc
    15661 tgctggcccc actggcgtgg gtaatgggtg ttgactggag tgatgcgaat cttgccggga
    15721 gtttgattgg acagaaactg gcaataaatg aatttgtcgc ttacctcaat ttctcacctt
    15781 atctgcaaac agctggcact ctggatgcta aaaccgtggc gattatttcc ttcgcgttgt
    15841 gcggtttcgc taactttggt tctatcgggg tggtggtggg ggcgttttct gcggttgcgc
    15901 cacaccgtgc accggaaatc gcccagcttg gtttacgcgc gctggcggcg gcgacacttt
    15961 ctaacctgat gagtgcaacc attgcaggat tctttattgg cttagcgtag tgcgcgagtt
    16021 atgagccgga taagatgtag tcaagcgtcg aatccggcaa cattgttaca ccatcggcac
    16081 taatgaaagc gcgttatcgg cagacagggt agggttgttt aagctgccgc tggcgcgtga
    16141 aattgccgcg caggccatcg caaaacgggc gctgtcacgg aaattgtttc cctccagaaa
    16201 gctatatacc aggcccgcca taaaaccatc gtcagcacca aaactgtcta ctgtcgtatg
    16261 cgctggcgct gtcagcaaaa attgctctcc atccttttcg ctgcaataaa ctgactcatc
    16321 gggcaaataa acaaacagtt gctgaacgcc ttgctgatgc aacgcattca ctgcggcatt
    16381 acggtcagca tcgctggtga tcgcctgtcc ccataaaatt tccagctccg gtaaagtggg
    16441 tttcagggtg tgaatatgcg ccagccagtg tttgattttg cccgctttga attctgaaac
    16501 ggtatcgaca aacaccggga tttcatcagc gagggtaaag acccattcca gcgcctcggc
    16561 tgtcaggtta caatcagcaa gtaccacgcc cgcatgacga agtaaatcgc gcgacccgtt
    16621 cagtagttgc ggtgtcaact gatccagcag atgggtatcg ttaatcgcca gcacggtttg
    16681 atcgtctcga ttggcaattg ccagatacgt cgatgtgctt tggccatgta aacgaacgca
    16741 gccggagaca ttcacgccgg cgcggcgcgt ttcttccagg agcatttcgc cataaaagtc
    16801 atcaccaatc actgaaagca aatggacgtc gcggcctaac agcgccaggt tgtgggcgat
    16861 attgcgtcca acgccgcctg ccgagcaatg aatggtaccg ggatgagaag ccgcttgcgg
    16921 gtaacggata tccgccatcc cgcgaatatc catattgatt gtccccacca ctacgcagta
    16981 ttcctgctcg gtgagaatgt aacctttgcc tttaatccgg cctttgcgca ttaaatccat
    17041 aatatgcgcc gcaacgcgcg aacggctgat ttgcagcata tccgcaattt cgttctgctg
    17101 aatcagcggg ttacgccgta aaattgcaag gatctccttc tcccggttgt tcatcttcag
    17161 gctaccgctt tttcagtttg ttgcgctttc agccaggcga tctcttctgc ccagatatcc
    17221 gggttgatgg tttcaaggat cagcggaatg ccgtcgaaac ggtcgtcctg catgatccag
    17281 cggaacgcat catgaccaat attgccttca ccgaggctat gatggcggtc aacgcggctg
    17341 ccaaaggtgc ttttcgcatc gttaaggtgc atcccgcgca gatacttaaa gccaacaata
    17401 cgggcaaaat ccgcgaacgt tttctcgcat tcggcagaag tacgcaaatc atacccggca
    17461 gcaaaagcat ggcaggtatc aatgcagacg ccgacgcggg atttatcttc cacgccgtcg
    17521 ataatcgccg cgagatgttc gaatttaaac cctaagttac tgccctgacc ggcggtgttt
    17581 tcaatcaccg ctgtcacacc ttgagtttta tccagcgcaa tgttgatgga ttcggcaata
    17641 cgcgcaaggc aatcctcttc tgaaatctgc atcagatggc tgccagggtg gaagttgagc
    17701 aaagaaagcc ccagctgttc gcaacgctgc atttcatcta taaacgcatc gcgcgatttt
    17761 tccagagctt cagcgaccgg atgtccgagg ttaatcagat aactgtcgtg gggaagaatt
    17821 tgcgccgatg tgtagtgata tttttcacag gcggctttga attcatcgat ggtttgcgtc
    17881 gtgagcggtg cggcacgcca ctgacgttgg tttttggtga acaaggcaaa cgcggttgcg
    17941 tcgatttcgg cggcgcgaat tgcggcattt gccagaccgc cagcagcact aacgtgcgct
    18001 ccaatgtatt tcatgcgagg actcctgtta aacccgctgg aggaaaacgg taatgatagc
    18061 gggttaacag gagcaagatg tagtggttta tgcgatgacg ctttgaatca catagttaat
    18121 cgcaccacca ccaacaatca gccaggcaaa cagtaccagt gccatcagca gaggtttcgc
    18181 cccggctttt ttcagcgcgc tgacgtgagt ggtcagaccc agcgccgcca tcgccattgc
    18241 cagcaggaag gtatccagcg ttaccagcat gttcaccacg ctctgcggta acaggtggaa
    18301 cgagttaaag atggcaacta cgatgaacaa gatggcaaac cacggaatag tgattttgct
    18361 tttctcgccg ctgttcgccc cagacagctg tttaacacgc gccgccagca ggatgaggaa
    18421 cggagccagc atcatcacgc gcagcatttt ggaaataact gctgcgtttt ctgcatccgg
    18481 gctgatggca tgacctgccg ccaccacctg cgccacttcg tgcacagtag aaccaatgta
    18541 gataccgaaa gtttccggac taaaccattg agacatcagc ggatatatcg ccgggtagag
    18601 gaaaatcgcg acggtcccga agataacaac ggttgcaaca gccacggtta ctttactggc
    18661 ttccgctttc actaccggct cagtcgccag taccgcggca gcaccacaga tactgctacc
    18721 ggcaccgatc aaccagctgg tgtgcttatc cagaccaaac actttctgcc ccaggaagca
    18781 agccagcagg aaggtactgg acagcgtcag cacgtcaatg atgatcccac tgataccgac
    18841 atcggcaatt tgcgagaacg tcagacggaa gccataaaga atgataccca gacgtaataa
    18901 atattgcttg gcaaacagca caccaccgtc acagcttttc cagatgtgcg gatagatggt
    18961 gttgcctaaa accatcccca acaagattgc gagggtgagg gcactaaacc cggcacccgc
    19021 aaccgcggga atggaaccac cccacagggc gaccccggtg ataactgcac tcagggctaa
    19081 ccccggaata aaatgccaca gtgtacgatg ttgtttctgt aaggtgatat tcgtcataac
    19141 cctctccttt aaccgggcat aaggttacga ctaactggtt taaaaataaa attgattata
    19201 tatttataat taatctttat aagtggtaag cgactatgca catcaccctc cggcagttgg
    19261 aagtttttgc agaagtattg aaaagtggat caaccaccca ggcgtcggtg atgctggcgt
    19321 tgtcgcaatc agcagtgagc gcagccttga ccgatctgga agggcagctt ggcgtgcaac
    19381 tgtttgatcg cgtggggaaa agactggttg ttaatgaaca cgggcggctg ctctatccgc
    19441 gcgcgttggc actgcttgaa caggcggttg aaatcgaaca actgtttcgc gaagacaacg
    19501 gcgcgattcg tatctatgcc agtagtacta tcggtaacta cattctgcct gcagtcattg
    19561 cccgttatcg ccatgattat ccgcagttgc cgattgaact tagcgttggg aacagccagg
    19621 acgtgatgca agcggtgctg gatttccgcg ttgatattgg ctttattgaa ggaccgtgcc
    19681 acagcactga aatcatttct gaaccgtggc tggaagacga gctggtggtt ttcgccgcgc
    19741 cgacttcgcc attggctcgt ggtccggtca ccttagaaca gctggccgct gcgccgtgga
    19801 tcctgcgtga acgcggctcc ggcacgcggg agattgtcga ttatctgttg ctgtcacatt
    19861 taccgaagtt tgagatggcg atggaattag gtaactccga ggcaatcaaa catgcggtgc
    19921 gtcatgggtt gggaattagt tgcctgtcgc gacgggtgat tgaagatcaa ttgcaggcag
    19981 gcacattaag tgaagttgcg gtccctctgc cgcgcctgat gcgtacgttg tggcgtatac
    20041 atcatcggca aaaacacctt tccaacgcgc tacggcgctt tctggactat tgcgatcccg
    20101 cgaatgtacc gcgttaagtt gctgtacaag aacatgctgg tgctgtgtcg atttcgtgac
    20161 gcagcgccct cagcatgcat tcgccagaaa agagattggc tgctttactt ataatccctg
    20221 ggcgatcatg aaggtgtctt ataaccgtgt atttctgccg gaaggattgc caatcgtctg
    20281 ctacaatcgc gcctcatttt taagatggat agcatttttg tatggtttcc gaaactaaaa
    20341 ccacagaagc gccgggctta cgccgtgaat taaaggcgcg tcacctgacg atgattgcca
    20401 ttggcggttc catcggtaca ggtctttttg ttgcctctgg cgcaacgatt tctcaggcag
    20461 gtccgggcgg ggcattgctc tcgtatatgt tgattggcct gatggtttac ttcctgatga
    20521 ccagtctcgg tgaactggct gcatatatgc cggtttccgg ttcgtttgcc acttacggtc
    20581 agaactatgt tgaagaaggc tttggcttcg cgctgggctg gaactactgg tacaactggg
    20641 cggtgactat cgccgttgac ctggttgcag ctcagctggt catgagctgg tggttcccgg
    20701 atacaccggg ctggatctgg agtgcgctgt tcctcggcgt tatcttcctg ctgaactaca
    20761 tctcagttcg tggctttggt gaagcggaat actggttctc actgatcaaa gtcacgacag
    20821 ttattgtctt tatcatcgtt ggcgtgctga tgattatcgg tatcttcaaa ggcgcgcagc
    20881 ctgcgggctg gagcaactgg acaatcggcg aagcgccgtt tgctggtggt tttgcggcga
    20941 tgatcggcgt agctatgatt gtcggcttct ctttccaggg aaccgagctg atcggtattg
    21001 ctgcaggcga gtccgaagat ccggcgaaaa acattccacg cgcggtacgt caggtgttct
    21061 ggcgaatcct gttgttctat gtgttcgcga tcctgattat cagcctgatt attccgtaca
    21121 ccgatccgag cctgctgcgt aacgatgtta aagacatcag cgtcagtccg ttcaccctgg
    21181 tgttccagca cgcgggtctg ctctctgctg cagcggtgat gaacgcagtt attctgacgg
    21241 cggtgctgtc agcgggtaac tccggtatgt atgcgtctac tcgtatgctg tacaccctgg
    21301 cgtgtgacgg taaagcgccg cgcattttcg ctaaactgtc gcgtggtggc gtgccgcgta
    21361 atgcgctgta tgcgacgacg gtgattgccg gtctgtgctt cctgacctcc atgtttggca
    21421 accagacggt atacctgtgg ctgctgaaca cctccgggat gaccggtttt atcgcctggc
    21481 tggggattgc cgttagccac tatcgcttcc gtcgcggtta cgtattgcag ggacacgaca
    21541 ttaacgatct gccgtaccgt tcaggtttct tcccactggg gccgatcttc gcattcattc
    21601 tgtgtctgat tatcactttg ggccagaact atgaagcgtt cctgaaagat accattgact
    21661 ggggcggcgt agcggcaacg tatattggta tcccgctgtt cctgattatt tggttcggct
    21721 acaagctgat taaaggaact cactttgtac gctacagcga aatgaagttc ccgcagaacg
    21781 ataagaaata agttttctcc cttccttgct aagccctctc aaccgagagg gctttttcaa
    21841 ttccatttcc ctgacaaatc atgcggatat aaaatttaac atttggattg ataattgtta
    21901 tcgtttgcat tatcgttacg ccgcaatcaa aaaaggctga caaatcagag gctggtctgg
    21961 ctttctggga tgatcacctg catacaaata agtccaccgc gatgctgccg tacgcaaggg
    22021 gacgtgaaga agatgtgagc gataacccat tttattttcg tagttacctc atggagatat
    22081 ggaatgttta ggttgaaccc tttcgtacgg gtcgggctgt gtttgtccgc tatttcttgt
    22141 gcatggcctg tgttagcggt cgatgatgat ggcgaaacga tggttgtcac tgcatcttcc
    22201 gttgagcaaa accttaaaga tgcacctgcc agtatcagcg tcattaccta ggaagacctg
    22261 cagcgaaaac cggtacagaa tctgaaggat gtcctcaaag aagtgcctgg cgtacaactg
    22321 acgaacgaag gggataaccg taagggcgtt agtattcgtg gtctggacag cagctacacc
    22381 ctgattctcg tcgacggtaa acgcgttaac tcccgcaatg ccgtcttccg ccacaatgat
    22441 ttcgatctga actggatccc ggtcgattcc atcgaacgta ttgaagtggt ccgtggcccg
    22501 atgtcgtcgc tgtacggttc cgatgcgctc ggcggtgtag tgaatatcat caccaaaaaa
    22561 atcggtcaga aatggtcggg caccgttacc gtcgatacca ccgttcagga acatcgcgat
    22621 cgcggtgata cctataacgg tcaattcttt accagcggac cattaattga cggcgtgctg
    22681 ggaatgaaag cttacggcag cctggcaaaa cgtgaaaagg atgacccgca aaactcaacg
    22741 accaccgata ccggagaaac gccgcgtatt gaaggattct ccagccgcga cggcaatgtc
    22801 gaatttgcct ggacaccgaa tcaaaatcac gattttactg ccggatacgg tttcgaccgt
    22861 caggatcgtg attccgactc gctggacaaa aaccgcctgg aacgccagaa ctactccgtc
    22921 agccataatg ggcgttggga ttacggcacc agcgaactga aatactacgg tgagaaagtc
    22981 gagaacaaaa accctggcaa cagcagcccg ataacttccg aaagcaatac ggtcgacggc
    23041 aaatacacgt tgccgctgac ggcgattaat cagtttctca cggttggcgg tgaatggcgt
    23101 cacgacaaac ttagcgatgc ggtgaacctg actgggggaa ccagctccaa aacatctgcc
    23161 agccagtacg cgctatttgt tgaagatgaa tggcggatct tcgagccgct ggcgctgacg
    23221 accggcgtac gtatggacga tcatgaaacc tacggtgaac actggagtcc gcgtgcctac
    23281 ctggtttata acgccaccga caccgtaacg gtgaaagggg gctgggcgac ggcatttaaa
    23341 gcaccttctc tgttgcaact tagccctgac tggacgagca attcctgccg tggcgcatgt
    23401 aagattgtgg gtagcccgga tctgaaacca gaaaccagcg aaagttggga gctggggctt
    23461 tactacatgg gcgaagaagg ctggctggaa ggggttgaat ccagcgttac cgttttccgt
    23521 aacgatgtga aagatcgtat cagcatcagc cgtacgtctg acgtcaatgc tgcaccaggc
    23581 taccaaaact ttgttggttt tacagcgaac ggtgtgccgg tatttagcta ctacaacgtt
    23641 aacaaagcac gtattcaggg cgtggaaacc gagctgaaaa ttccgttcaa cgatgaatgg
    23701 aaactgtcac tcaactacac ctacaacgat ggtcgtgatg tcagcaacgg cgaaaataaa
    23761 cctttatccg atctgccgtt ccataccgct aacggtacgc tggactggaa accgctggcg
    23821 ctggaagact ggtcattcta tgtttccgga cactataccg ggcagaaacg cgccgacagc
    23881 gcgacggcta aaacaccggg cggttatacc atctggaata ccggtgcggc ctggcaggtg
    23941 actaaagacg tcaaactgcg cgcaggcgtg ctgaaccttg gcgacaagga tctcagtcgt
    24001 gacgactaca gctataacga agacggacgt cgttacttta tggcagtgga ttatcgcttc
    24061 tgatgagaag atgcccggcg aaccgggcgg actttcactt cagtaaatac tgcgcatgga
    24121 agcgcaggtg atcctctata aaagaggcga tgaagtagta actgtgatca tatcccggct
    24181 gaatacgcag cgtcatcggc cacgctttct ggcgggcggc ttctgccagc actgcgggtt
    24241 gcagctggtc ggcaagaaac tgatcattat caccctgatc gataagcgtc gggatcgcat
    24301 cctgcgcgtt actggcatac atcagcgcac aactgtccca ttccagccat gcatttttgt
    24361 cctcacctaa atagctgcta aacgctttaa tgccccaggg tacgctgcat ggattcacaa
    24421 tcggcgcaaa ggcggaaacg ctggtgtatt tgcccgggtt tttcagcgcc ataatcagcg
    24481 caccgtgacc acccattgaa tgaccgctaa tagcgcagcg gtcgctgaca ttaaattgcg
    24541 actgaaccag cgccggtaat tcatcgcgca gataatcata catccggtaa tgcgtcgccc
    24601 acggcggttg cgtggcatta agataaaagc ctgcgccctg gcctaaatcg tagccatcgt
    24661 cgttggcaac ctgttcgccg cgcgggctgg tgtctggcat caccagtaca atccccagtt
    24721 ctgccgccac gcgctgggca cccgccttgg tggtgaagtt ctcgtcattg caggttaatc
    24781 cggaaagcca gtacagcact ggtggcgggg tgtgatcacg aggtggtggg agaaagatac
    24841 tgaacgtcat cggacagttt aaggtactgg agtcgtgtcg ccagcgttgc tgccagcctt
    24901 caaaacagcg gtgctcttcg agcatttcca tgcgtggctc cttgttgtgt tgtttgcaat
    24961 ttcttcatca taccgtttat tcaatgtgct gtgagtaact ttcacttccg tatttgcata
    25021 acgatgtttt aacatctgct gatgaaaggc agcggcaatt acaataatta tcgctgtgaa
    25081 tactggatta tgtgcgccgc ctcacgcaca ataatcaggc tgtaaatcag cttaataact
    25141 ttgcccccac gcagggcgga ggcgtcacac ctgcaggaga aatcataaat gccatcactc
    25201 agtaaagaag cggccctggt tcatgaagcg ttagttgcgc gaggactgga aacaccgctg
    25261 cgcccgcccg tgcatgaaat ggataacgaa acgcgcaaaa gccttattgc tggtcatatg
    25321 accgaaatca tgcagctgct gaatctcgac ctggctgatg acagtttgat ggaaacgccg
    25381 catcgcatcg ctaaaatgta tgtcgatgaa attttctccg gtctggatta cgccaatttc
    25441 ccgaaaatca ccctcattga aaacaaaatg aaggtcgatg aaatggtcac cgtgcgcgat
    25501 atcaccctga ccagcacctg tgaacaccat tttgttacca tcgatggcaa agcgacggtg
    25561 gcttatatcc cgaaagattc ggtgatcggt ctgtcaaaaa ttaaccgcat cgtgcagttc
    25621 tttgcccagc gcccacaggt acaggaacgt ctgacccagc aaattctcat tgcgctacaa
    25681 acgctgctgg gcaccaataa cgtggctgtc tctatcgacg cggtgcatta ctgcgtgaaa
    25741 gcgcgtggca tccgcgacgc aaccagtgcc acaacaacga cctctcttgg tggattgttc
    25801 aaatccagtc agaatacgcg ccacgaattt ctgcgcgctg tgcgtcatca caactgatta
    25861 aaaggcagga accatggggc gcaacgtcac gctcgatttt gttcgcggcg tcgccattct
    25921 ggggatcctg ctattaaaca tcagcgcctt tgggctacca aaggcggctt atcttaatcc
    25981 cgcctggtac ggcgctatta cgccgcagga tgcatggacc tgggcatttc tcgatctcat
    26041 cggccaggtg aaattcctca cgctttttgc gctgctgttt ggtgctggcc tgcaaatgtt
    26101 gctgccccgt ggcagacgct ggatccagtc gcggttaacg ctgttagtct tgctgggctt
    26161 tattcacggt ttattgttct gggacggcga tattctgctg gcttacgggc tggtgggctt
    26221 aatctgctgg cggctggtgc gcgatgcgcc atcggtaaaa agcctgttta ataccggcgt
    26281 catgctttat ctggtggggc ttggcgtttt gctgttattg gggttgattt ccgatagcca
    26341 gaccagccgc gcctggacac ctgatgcatc ggctattttg tatgaaaagt actggaaact
    26401 tcacggtggt gttgaagcga tcagtaatcg tgccgatggt gttggcaaca gtttactggc
    26461 actgggcgca cagtatggct ggcaactggc agggatgatg ctcattggtg cggcgctgat
    26521 gcgcagcggc tggctgaaag ggcagttcag cttacgtcac tatcgtcgta ctggttttgt
    26581 gctggtggcg attggggtga ccattaacct tcctgccatc gccctgcaat ggcagctgga
    26641 ctgggcgtat cgctggtgtg cctttttact tcagatgccg cgggaactga gtgcgccgtt
    26701 tcaggcgatt ggctatgcgt cgctgtttta tggcttctgg ccgcaattga gccgctttaa
    26761 gctggtgctt gcgatcgcct gcgtcggacg gatggcgctg accaactatc tattgcaaac
    26821 gctgatttgt accacgcttt tttaccacct cggtttgttt atgcattttg accgcctgga
    26881 gctgctggcg tttgttattc cggtatggct ggcgaatatc ctcttctctg ttatctggct
    26941 gcgttacttc cgccaggggc cggtggaatg gctctggcgt cagttaactt tgcgtgcagc
    27001 tgggccggca agatctaaaa catcaagata acgatctgga tcacattcgt taacaaaacg
    27061 gctgtaaccg tttccattgc tgtgactcga ttcacgaagt cctgtattca gtgctgacaa
    27121 aatagccgcc agcaagcagt catttactgc aatctcataa caggtagtga atatgatcac
    27181 cattcgtgat gtagcgcgtc aggctggcgt ctctgtggca acggtttccc gggtgctcaa
    27241 taacagcacg ctggtcagtg ccgacacgcg tgaagcagta atgaaagccg tgagtgagct
    27301 ggattatcgg ccaaacgcca atgcccaggc gctggcaact caggttagcg ataccattgg
    27361 cgtggtggtg atggacgttt ctgatgcgtt ttttggtgcg ctggtaaaag cggtggattt
    27421 ggtcgcccag cagcatcaga aatacgtgct aataggcaat agctatcatg aagcggaaaa
    27481 agagcgtcac gccattgagg tgttaattcg ccagcggtgt aatgcgttga ttgttcactc
    27541 aaaagcattg agtgatgatg aactggcaca atttatggat aacattcctg gtatggtgtt
    27601 aatcaaccgc gttgtgccgg ggtacgccca tcgttgcgtt tgcctggata atctcagcgg
    27661 tgcccgaatg gcgacgcgca tgttgctgaa taacggtcat caacgtattg gttatctttc
    27721 ttccagtcac ggcattgaag atgacgccat gcgtaaagca ggctggatga gtgcgttgaa
    27781 agagcaggat attattccgc cggaaagctg gattggcaca ggtacgccgg acatgccggg
    27841 cggtgaggcg gcgatggttg aactgctggg gcgcaatcta caacttaccg ctgtatttgc
    27901 ttataacgac aatatggccg ctggcgcact gacagcatta aaagataatg gcattgcgat
    27961 tccgttacat ctctccatca tcggtttcga tgatattccc atcgcccgtt acaccgaccc
    28021 gcaattaacg actgtgcgtt atcccattgc ttcaatggct aaattagcca ccgaactggc
    28081 cttgcagggg gcagcaggca atattgatcc gcgtgccagc cactgtttta tgccgacgtt
    28141 agtgcgtcgc cattctgtcg caacgcgcca gaatgcggcg gcgatcacta actcaacaaa
    28201 tctggcgatg taaccgcttt caatctgtga gtgatttcac agtatcttaa caatgtgata
    28261 gctatgattg caccgtttta acgttgtaac ccgtatgtaa cagtgaataa tcactttcac
    28321 cgaggtaata gcgtcataac aacaattaaa gccgttttct ggagcgttac cgggcatgga
    28381 agaacgaatt ttaaaaagtg agcttcggcg ttcagtaaca cttcattaac tctactgccc
    28441 cgccgagcat ttatctcaag cactaccctg cataagaaaa accggagata ccatgaataa
    28501 gaaggtgtta accctgtttg ctgtgatggc cagcatgtta ttcggtgccg ctgcacacgc
    28561 tgctgatact cgcattggtg taacaatcta taagtacgac gataacttta tgtctgtggt
    28621 gcgcaaggct attgagcaag atgcgaaagc cgcgccagat gttcagctgc tgatgaatga
    28681 ttctcagaat gaccagtcca agcagaacga tcagatcgac gtattgctgg cgaaaggggt
    28741 gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc
    28801 gcgtgggcaa aacgtgccgg tggttttctt caacaaagaa ccgtctcgta aggcgctgga
    28861 tagctacgac aaagcctact acgttggcac tgactccaaa gagtccggca ttattcaggg
    28921 cgatttgatt gctaaacact gggcggcgaa tcagggttgg gatctgaaca aagacggtca
    28981 gattcagttc gtactgctga aaggtgagcc gggccatccg gatgcagaag cacgtaccac
    29041 ttacgtgatt aaagaattga acgacaaagg catcaaaact gaacagttac agttagatac
    29101 cgctatgtgg gataccgctc aggcgaaaga taagatggac gcctggctgt ctggcccgaa
    29161 cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga
    29221 agcactgaaa gcacacaaca agtccagcat tccggtgttt ggcgtcgatg cgctgccaga
    29281 agcgctggcg ctggtgaaat ccggtgcact ggcgggcacc gtactgaacg atgctaacaa
    29341 ccaggcgaaa gcgacctttg atctggcgaa aaacctggcc gatggcaaag gtgcggctga
    29401 tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta ccttatgttg gcgtagataa
    29461 agacaacctg gctgaattca gcaagaaata agactgattg ggcgcagtcg tttactgcgc
    29521 ccttattgta tggccaacaa ggtaaaatta tatggtcagc tcaacgactc cgtcttccgg
    29581 ggaatacttg ttggaaatga gcggtatcaa caagtctttt ccaggtgtta aggcacttga
    29641 taacgttaat ttaaaagtcc ggccacattc tatccatgca ttaatggggg aaaacggcgc
    29701 aggaaaatca acattattaa aatgcctgtt tggtatttat caaaaagact ccggcaccat
    29761 tttattccag ggtaaagaga tcgatttcca ttctgccaaa gaagcgctgg aaaatggtat
    29821 ttcgatggta caccaggagt taaacctggt attacaacgt tcggtgatgg acaacatgtg
    29881 gctggggcga tatcccacca aaggcatgtt tgtcgatcag gacaaaatgt accgcgaaac
    29941 caaagcgatt tttgatgaac tggatattga tatcgatccg cgtgcgcgcg tcggcacatt
    30001 atccgtttcg caaatgcaga tgatcgaaat cgccaaagcg ttttcctata acgcgaaaat
    30061 tgtgattatg gatgaaccga cttcttcgtt aaccgaaaaa gaggtcaatc atctgttcac
    30121 tattattcgt aaattaaaag agcgcggctg cggtattgtt tatatctcac ataaaatgga
    30181 agaaatcttc cagttatgtg atgaagttac cgtattgcgc gacggtcagt ggatcgccac
    30241 tgaaccgctg gcaggactga cgatggacaa gatcatcgcc atgatggttg ggcgttctct
    30301 taaccagcgt ttccctgaca aagaaaacaa gccgggcgaa gtgattcttg aagtgcgtaa
    30361 cctgacgtcg ctgcgccagc cgtcgattcg cgatgtctcg tttgatctgc ataaagggga
    30421 gattctcggt atagccggtc tggtgggagc gaaacgtacc gatattgttg agacgttatt
    30481 tggtattcgc gagaaatcgg ctggcaccat tacgttgcac ggcaaaaaga tcaataacca
    30541 taatgccaac gaagccataa accacggatt tgcactggta actgaagagc gccgctcaac
    30601 gggaatttat gcctatctgg atatcggttt taactcgtta atttccaata ttcgcaacta
    30661 caaaaataaa gttggtttac tggataactc gcggatgaaa agcgataccc agtgggtgat
    30721 tgattcgatg cgggtaaaaa caccggggca tcggacgcaa attggttcgc tctccggcgg
    30781 taatcagcaa aaggtgatta ttggtcgctg gctactaacg caaccagaaa tattaatgct
    30841 cgatgaaccg acgcgcggta ttgatgtcgg agcgaagttt gagatctatc agttaattgc
    30901 cgaactggcg aagaaaggca aagggattat tattatctcc tctgaaatgc ctgagttgtt
    30961 agggataaca gaccgtattc tggtcatgag caatggtctc gtttccggaa ttgtcgatac
    31021 aaaaacaaca acgcaaaacg aaattctgcg tcttgcgtct ttgcaccttt aagattaggg
    31081 gcttcccatg agtgcgttaa ataagaaaag ttttcttact tacctgaaag agggcggtat
    31141 ttacgtcgtt cttttagttt tgctggcaat tattattttc caggacccaa catttttaag
    31201 tctgttgaac ttaagtaata ttctcaccca gtcatcggtg cgtattatta tcgcgctcgg
    31261 tgtggcaggg ttaattgtca cccaggggac cgacctttct gcaggtcgcc aggtggggct
    31321 ggcggcagta gtagcggcga cgttattgca gtccatggat aacgccaaca aagtgttccc
    31381 ggaaatggcg acgatgccga ttgcgctggt tattctgatt gtctgtgcca ttggtgcggt
    31441 gatcggtttg atcaacggtc tgattatcgc ctatctcaac gtgacgccgt tcattaccac
    31501 gctcggcacg atgatcatcg tctatggcat caactcgctc tattacgact ttgtcggggc
    31561 gtcgccgatt tctggtttcg acagtggctt ctctaccttt gctcagggct ttgtcgcgct
    31621 ggggagtttc cgtctctctt acatcacctt ctacgcgttg attgcggtgg cgttcgtctg
    31681 ggtgttgtgg aacaaaaccc gcttcggtaa gaacattttt gccattggcg gtaacccgga
    31741 agcggcgaaa gtatctggtg tcaacgtcgg cctgaacctg ctgatgatct acgcgttgtc
    31801 tggcgtgttc tatgccttcg gcgggatgtt agaagccgga cgtatcggct ctgccaccaa
    31861 caacctcggc tttatgtatg agttggatgc tatcgcggca tgtgtggtgg gtggtgtatc
    31921 gttcagcggt ggtgtaggga cggtgattgg cgtggtgacc ggggtgatta tttttaccgt
    31981 catcaactat ggcctgacgt acatcggcgt aaacccatac tggcagtaca tcatcaaagg
    32041 ggcgattatt atcttcgccg tggcgctgga ttcactgaaa tacgcgcgta agaaatgatg
    32101 tgaatgtggg tattgccgga tgcgccgcat ccggcaatcg gtgccaaatg ccggatgcgt
    32161 ctctggtttt acaacgttac cgggtgttct ttctctccct tcttaaactt cacttctccg
    32221 agatcaatac aacccaccgg gcagacgtga ccacaaagca ggcaaccaac acatttctcg
    32281 gtattacaat gcggtgtacg ggttttctcg ctccattcca tcgcctggtg accgccgtcg
    32341 taacaggaaa tataacagcg tccacagcca acacatttat cgagattgat atggggatag
    32401 acaatataac tgcggtctaa atcttccgcc gggacaatat tgttattcgc cagacctacc
    32461 atctcctgca acgaatcaaa cccttgatcg gcgagataat gcgacaaccc gcttgccata
    32521 tcttccacta tccgataccc gtactgcata atgccggtgg tcacctgcaa cgtcgctgca
    32581 ccgagcaata aaaactccgc cgcatcttcc caggtttcaa tgccgccgat accgctgatc
    32641 gggaaatcgc gcagttctgg atgggtacgc atttgctgaa tgaagcgcag ggcgatcggt
    32701 tttaccgctt taccggaata tccggaaata ctcgatttac cgttaacgat cggcataccg
    32761 attttctgat taagatcgat attggtgatg gatttaacgg tgttaatcgc cgcaatgcca
    32821 tctgcgccgc cgcgcttcgc cgccagcgcc acttcgcaca tatcaccgat attcggcgtc
    32881 atcttcgcca gcattggcag cgtggaacca cgttttactg cccgacaata tttttctacc
    32941 agctccgggc tttgcccgac atcgctaccc atcgcatgag aagtcatttg cggacaggag
    33001 aagttacact cgatcatatc cgcgccagct tcttgcacca gacgcgccag ctcttcccat
    33061 tgctgctcat tttcgcccat gattgaagcg atcaatactt tgtccgggta atcttctttc
    33121 agccgacgca gtgcggccag attatcttcc aacggatgtt cagcaatctg ctccatattt
    33181 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    33241 ttggcgataa aaaaaccgat cgttttaaac accacgccac cccagcctgt gtcgtaggct
    33301 ttggcacaca tctcatagca gttgcctaca ggcgaagaag agaggcagaa cgggttggga
    33361 aacttcacac cacaaaaggt aatcgaaaga tctttcgtta acatgagcaa gctcccttta
    33421 aatagtgatg aatcgcctca gcggcttctt tcccggtttt cacggcataa accacggttt
    33481 tgtcaccctc aacaatatcg ccagcagcaa agacttgcgg gtcgcgggtc tggtaatttt
    33541 gtgttttgat ggtgttacgc tgcggctcta actccgcaaa ggcatccagt ctggcatgct
    33601 gaccgacggc gagaataatt ttatctgccg ccatcgtcag ttcgcccggt aaccgtacat
    33661 gcttaaacgt gactttattg ccttccacag ctactggcgt gaatccatcg atgatcgaaa
    33721 cgcccagctc ccgggcgctg gcaaactctt tttcgcttgc cggaaactca tctaactctt
    33781 cacgcgctac gcaagttact gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    33841 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    33901 cctgtcgtgc acgttgcaag aagtcgacgg caatctcaac atcgctatgc tcaaacagtg
    33961 gtagccccga accgctcgat aaccccacag tgaccagtac cgcgcggttt tccgctttca
    34021 gctgctccag ggtgagtgtg ttaccgattt cgttattgca tttaatggtc acgcccattt
    34081 tttcaatacg ggcgatttct gcatccagta ctgattgcgg taaccggaat tgcggaatgc
    34141 cgttacgcaa ccagccaccg ggttgtgctt ctttctcata gatcgtgacg tcataaccct
    34201 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc cgcgccaata atggcgactt
    34261 tgccgagcgt tttagtaccg ggctgataaa tttccatccc agtttgttgt tcaaaatcag
    34321 taacaaaacg ctgtaagcga ccgatatcaa tgggagtatc aacaccggca cgggtacaac
    34381 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    34441 tttcacgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    34501 tccccggatc ggtctgtgcc gggcaagcct gactacaggg ggcgtcatgg cataataaac
    34561 agcgagaggc ttctttaatc gccagtaacg gtgtaaaagc cggagtgagt tcatccagat
    34621 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattaaggcg aacgctaaat
    34681 atactcgtca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta
    34741 cttatgtaag ctcctgg
//
LOCUS       DAZGAP010000023        34712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGAP010000023 DAZGAP010000000
VERSION     DAZGAP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.023"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            142..1764
                     /gene="pckA"
                     /locus_tag="ACHLAM_000986"
     CDS             142..1764
                     /gene="pckA"
                     /locus_tag="ACHLAM_000986"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO7852552.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            1879..2196
                     /locus_tag="ACHLAM_000987"
     CDS             1879..2196
                     /locus_tag="ACHLAM_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7852553.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            2255..2551
                     /gene="nadS"
                     /locus_tag="ACHLAM_000988"
     CDS             2255..2551
                     /gene="nadS"
                     /locus_tag="ACHLAM_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO7852554.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(2583..3935)
                     /gene="envZ"
                     /locus_tag="ACHLAM_000989"
     CDS             complement(2583..3935)
                     /gene="envZ"
                     /locus_tag="ACHLAM_000989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO7852555.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(3932..4651)
                     /gene="ompR"
                     /locus_tag="ACHLAM_000990"
     CDS             complement(3932..4651)
                     /gene="ompR"
                     /locus_tag="ACHLAM_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO7852556.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4879..5355
                     /gene="greB"
                     /locus_tag="ACHLAM_000991"
     CDS             4879..5355
                     /gene="greB"
                     /locus_tag="ACHLAM_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO7852557.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            5452..7773
                     /gene="yhgF"
                     /locus_tag="ACHLAM_000992"
     CDS             5452..7773
                     /gene="yhgF"
                     /locus_tag="ACHLAM_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO7852558.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            8211..8438
                     /gene="feoA"
                     /locus_tag="ACHLAM_000993"
     CDS             8211..8438
                     /gene="feoA"
                     /locus_tag="ACHLAM_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO7852559.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            8455..10776
                     /gene="feoB"
                     /locus_tag="ACHLAM_000994"
     CDS             8455..10776
                     /gene="feoB"
                     /locus_tag="ACHLAM_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO7852560.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            10776..11012
                     /gene="feoC"
                     /locus_tag="ACHLAM_000995"
     CDS             10776..11012
                     /gene="feoC"
                     /locus_tag="ACHLAM_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO7852561.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            11215..12096
                     /gene="rpnA"
                     /locus_tag="ACHLAM_000996"
     CDS             11215..12096
                     /gene="rpnA"
                     /locus_tag="ACHLAM_000996"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO7852562.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVPLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            12168..12287
                     /locus_tag="ACHLAM_000997"
                     /pseudo
     CDS             12168..12287
                     /locus_tag="ACHLAM_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12316..13086)
                     /gene="bioH"
                     /locus_tag="ACHLAM_000998"
     CDS             complement(12316..13086)
                     /gene="bioH"
                     /locus_tag="ACHLAM_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO7852563.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKMVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            13124..13807
                     /gene="gntX"
                     /locus_tag="ACHLAM_000999"
     CDS             13124..13807
                     /gene="gntX"
                     /locus_tag="ACHLAM_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO7852564.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13866..14441
                     /gene="nfuA"
                     /locus_tag="ACHLAM_001000"
     CDS             13866..14441
                     /gene="nfuA"
                     /locus_tag="ACHLAM_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO7852565.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            14802..16118
                     /gene="gntT"
                     /locus_tag="ACHLAM_001001"
     CDS             14802..16118
                     /gene="gntT"
                     /locus_tag="ACHLAM_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7852566.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(16163..18247)
                     /gene="malQ"
                     /locus_tag="ACHLAM_001002"
     CDS             complement(16163..18247)
                     /gene="malQ"
                     /locus_tag="ACHLAM_001002"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO7852567.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTFTQEDQRVHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQTRDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGSETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(18257..20650)
                     /gene="malP"
                     /locus_tag="ACHLAM_001003"
     CDS             complement(18257..20650)
                     /gene="malP"
                     /locus_tag="ACHLAM_001003"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO7852568.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWTNDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            21272..23977
                     /gene="malT"
                     /locus_tag="ACHLAM_001004"
     CDS             21272..23977
                     /gene="malT"
                     /locus_tag="ACHLAM_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO7852569.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGDWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWHNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVTHRQAAVQHAQKLLKMMGYGV"
     gene            complement(24020..25036)
                     /gene="rtcA"
                     /locus_tag="ACHLAM_001005"
     CDS             complement(24020..25036)
                     /gene="rtcA"
                     /locus_tag="ACHLAM_001005"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO7852570.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(25040..26266)
                     /gene="rtcB"
                     /locus_tag="ACHLAM_001006"
     CDS             complement(25040..26266)
                     /gene="rtcB"
                     /locus_tag="ACHLAM_001006"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO7852571.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            26455..28053
                     /gene="rtcR"
                     /locus_tag="ACHLAM_001007"
     CDS             26455..28053
                     /gene="rtcR"
                     /locus_tag="ACHLAM_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO7852572.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(28035..28793)
                     /locus_tag="ACHLAM_001008"
     CDS             complement(28035..28793)
                     /locus_tag="ACHLAM_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO7852573.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(28810..29640)
                     /gene="glpG"
                     /locus_tag="ACHLAM_001009"
     CDS             complement(28810..29640)
                     /gene="glpG"
                     /locus_tag="ACHLAM_001009"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO7852574.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(29685..30011)
                     /gene="glpE"
                     /locus_tag="ACHLAM_001010"
     CDS             complement(29685..30011)
                     /gene="glpE"
                     /locus_tag="ACHLAM_001010"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO7852575.1"
                     /translation="MEQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWRRQFPA
                     EVAYGA"
     gene            30201..31706
                     /gene="glpD"
                     /locus_tag="ACHLAM_001011"
     CDS             30201..31706
                     /gene="glpD"
                     /locus_tag="ACHLAM_001011"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO7852576.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(31760..32365)
                     /locus_tag="ACHLAM_001012"
     CDS             complement(31760..32365)
                     /locus_tag="ACHLAM_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852577.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHYWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(32368..33144)
                     /locus_tag="ACHLAM_001013"
     CDS             complement(32368..33144)
                     /locus_tag="ACHLAM_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852578.1"
                     /translation="MQPSSAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAIIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQFILKERARQA
                     IENLLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(33147..33857)
                     /locus_tag="ACHLAM_001014"
     CDS             complement(33147..33857)
                     /locus_tag="ACHLAM_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852579.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            complement(34031..>34712)
                     /locus_tag="ACHLAM_001015"
     CDS             complement(34031..>34712)
                     /locus_tag="ACHLAM_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7852580.1"
                     /translation="YSTNYPADRSKADYWSSGTEGYIKIRLDKKIISDVSLNNVLLVS
                     LYASQRQTEHGPVPLFNAWISNLDIQVPQGCTINEGTSFTVNMPDVWASELSRAGAGA
                     KPAGVTPVATTIPINCTNKDTDAVMTLVFDGNISATRDTNGKQSIIQAQDNPDVGIMI
                     MDSQQNSVDLNALATSVGVPFRLVENQAASAQTADVTFLALPVSTTGKVPAAGRYNAL
                     AVLRVEYQ"
BASE COUNT         8401 a         9351 c         8727 g         8233 t
ORIGIN      
        1 accgtttcgt gacaggaatc acggagtttt ttgtcaaata tgaatttctc cagatacgta
       61 aatctatgag ccttgtcgcg gttaacaccc ccaaaaagac tttactattc aggcaataca
      121 tattggctaa ggagcagtga aatgcgcgtt aacaatggtt tgaccccgca agaactcgag
      181 gcttatggta tcagtgacgt acatgatatc gtttacaacc caagctacga cctgctgtat
      241 caggaagagc tcgatccgag cctgacaggt tatgagcgcg gggtgttaac taatctgggt
      301 gccgttgccg tcgataccgg gatcttcacc ggtcgttcac caaaagataa gtatatcgtc
      361 cgtgacgata ccactcgcga tactttctgg tgggcagaca aaggcaaagg taagaacgac
      421 aacaaacctc tctctccgga aacctggcag catctgaaag gcctggtgac caaacagctt
      481 tccggcaaac gtctgttcgt tgtcgacgcc ttctgtggtg cgaacccgga tactcgtctt
      541 tccgtccgtt tcatcaccga agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt
      601 cgcccgagcg atgaagagct ggcaggtttc aaaccagact ttatcgttat gaacggcgcg
      661 aagtgcacta acccgcagtg gaaagaacag ggtctgaact ccgaaaactt cgtggcgttt
      721 aacctgaccg agcgcatgca gctgattggc ggcacctggt acggcggcga aatgaagaaa
      781 gggatgttct caatgatgaa ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc
      841 tccgccaacg ttggcgagaa aggcgatgtt gcggtgttct tcggcctttc cggcaccggt
      901 aaaaccaccc tttccaccga cccgaaacgt cgcctgattg gcgatgacga gcacggctgg
      961 gacgatgacg gtgtgtttaa ctttgaaggc ggctgctacg caaaaactat caagctgtcg
     1021 aaagaagcgg aacctgaaat ctacaacgct atccgtcgtg atgcgctgct ggaaaacgtc
     1081 accgtgcgtg aagatggcac tatcgacttt gatgatggtt caaaaaccga gaacacccgc
     1141 gtttcttatc cgatctatca catcgaaaac atcgttaagc cggtgtccaa agcgggccac
     1201 gcaactaaag ttatcttcct gaccgcagac gcctttggtg tgctgccgcc ggtttctcgt
     1261 ctgactgccg atcaaaccca gtaccacttc ctctctggct tcaccgccaa actggccggt
     1321 acagagcgcg gcatcaccga accgacgcca accttctccg cctgcttcgg cgcggcattc
     1381 ctgtcgctgc acccgacgca gtacgcagaa gtgctggtga aacgtatgca ggcggcaggc
     1441 gcgcaggcgt atctggttaa caccggctgg aacggcactg gcaaacgtat ctccattaaa
     1501 gatacccgcg ccattatcga cgccatcctc aacggttcgc tggataacgc agaaaccttc
     1561 actctgccga tgtttaacct ggcgatccca accgaactgc cgggcgtaga caccaggatc
     1621 ctcgatccgc gtaacaccta cgcctctccg gaacagtggc aggaaaaagc tgaaacgctg
     1681 gcgaaactgt ttatcgacaa cttcgataaa tacaccgaca cccctgcggg tgccgcgctg
     1741 gtagcggcag gtcctaagct gtaatcagct gatatctcat tgacccctta tccaaacgga
     1801 taaggggttt ttttatctaa ttagctacca atatgtacac ggtacactta taattatcaa
     1861 tagaccgcag gatgcattat gttcaccttt attgaactac aaggatttag caaacgccgc
     1921 cagttattgc tacctgatga tgagttccgg gcttttcagg aactactcat tcaagatccc
     1981 actgccggtg acattatcgc aggcacaggc agttttagaa aaattcgctg gaaccgttct
     2041 ggaatgggga aacggggtgg cattcggata atttattaca acgtaacacg caatggccga
     2101 atttatctgg cgttgattta tcctaaaaat gaacaggatg atttaacaga agaacaaaga
     2161 aaggcattga aactattgtc cgagaagctg ctttaatgtt gaagctccac aaattccacc
     2221 atggccgaaa tgtcaacgga ggcattgaaa acagatgaaa gatgaattat ttgctgatct
     2281 tcttgccagc gcagaagaaa tggtacgcat cgaaaagggt gaagaaacgc ctgaaccaga
     2341 gcacgtccat acttttagcg aaatcgatgt aaaagcgatc cgtgaagcaa cgggtttacg
     2401 tcagcaagat tttgctgtcg ccgtaggggt aagttatgac ctggtcaaaa gctgggaaac
     2461 caaacgccgt caacctaccg gagcaccccg aaaattgctc ctgcttttgc aagcgaatcc
     2521 tttcattatc aatcaattga aaacaatcta aaaacaggag gcaccttcgc ctcccgtttt
     2581 atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg gcactggcag ccaggcgcga
     2641 atggaaagcc cgccccgctc gctgctgcca agctccagca tgccgttatg gttatccacg
     2701 atacgctgca caatcgccag ccctaatccc gtgccgctaa tggtgcgcgc actgtcgccg
     2761 cgaacaaacg gctggaacag gtgcttacgt tgttccggcg caattcccgg accatcatct
     2821 tccacctgga accaggcgcg attcggctcc gttccgctgc tgactttgat ccagccattg
     2881 ccatagcggg cagcgttcac caccatattc gccaccgcac gtttgatcga cagcgggtgc
     2941 attttcacct caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc atagccactt
     3001 tcggcggcaa tcacctcacc gagcactgcg ttgagatccg ccatttccat cggcatctcc
     3061 tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg cgttgcactc ttcgatatct
     3121 ttattgatcg attcggccag gtagccatcc tgctcgctca tcatctcggt cgccaggcga
     3181 atgcgcgtca gcggcgtacg cagatcatga cttacccccg ccatcagcag cgtgcgatca
     3241 tccgccagtt gcttaacacc agccgccata tggttaaagg cgcgggtaac ggaacgcacc
     3301 tccgaagcgc catattcacg cagcggcggc ggaataatcc ctttaccgac ctgcagagca
     3361 gcatgttcga gatcgaccaa cggtcggttc tggatacgga taaacagcca cgccccgcct
     3421 atcgccaata gcataatcgc cagcgtatag cggaacagcg gagaaaaatc accctgatga
     3481 atttcggtca gcggcacgcg cacccagata ttgggcgaca gccaggtttt cagccagaca
     3541 acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag ttgctgcgcc
     3601 atctgatggc ttaagaattc atagtgttgt gcccaacgca gacctgcctc ttcggcagct
     3661 tcgttggaat agagagagat ccccagctca cggtagatct cccgacggaa agcgggaggc
     3721 acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg tcatcaacat acgcacttcg
     3781 tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt cagcaccacc
     3841 agataagtcg tcaccaggct gacgaacagc aaggtgacga tgagcaataa cgtacgggca
     3901 aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agaaccgtcc ggtacaaaga
     3961 cgtagcccag accccagacg gtctgaatgt aacgcggatg cgccggatct tcttccacca
     4021 tgcggcgtag acgcgaaatc tgcacgtcga tggagcgttc cattgcggag tattcacggc
     4081 cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg ctgaccagtg
     4141 ccttcagtac cgcaaactca ccgctggtga gcggcatcgg ctcatcttcg cggaacattt
     4201 cacgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgcctct tcctgtgacg
     4261 gcgcgcccgg cagttcgttg gcctgacgac gcagcaccgc acgaatgcgg gccagcaatt
     4321 cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc aatctccagg ccaacgatac
     4381 ggtccacttc ttcccctttc gccgtcacca taatgatcgg catcgggttg ctctggctac
     4441 gaagacgtcg gcaaatcgac aagccatctt caccaggtaa catcaaatcc agtaccataa
     4501 gatggaaaga ttcacgagtc agcaggcgat ccatctgttc tgcattagcg acgcttcgaa
     4561 cctggaagcc ttgttcggtg agataacgtt ccagcagcgc gcgcaggcgc atatcgtcat
     4621 cgaccaccag aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt tcgcaacaat
     4681 ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc agctaaatct ggtatgaatt
     4741 tcagcctaaa ttgttacaaa gcatattaaa cagcagctta agtatacaat ttattcggcg
     4801 aaacattatt gattctgttg atatgatcac gttataccca atgtgcgcat tatcaaacag
     4861 acaaagggaa tcaacgagat gaaaacgccc ctggttaccc gggaagggta tgaaaaactc
     4921 aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa aaaggtgacc
     4981 tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact atcagtataa taaaaagcgc
     5041 ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat gcatggaaaa tctcaaaatc
     5101 gtcgattact cccctcagca ggaaggcaaa gtcttttttg gtgcgtgggt ggagattgaa
     5161 aacgacgatg gcgtgactca ccgtttccgt attgtcggct acgatgaaat ttttggccgt
     5221 aaagattaca tctctatcga ttccccgatg gctcgcgcat tgttgaaaaa agaggtgggc
     5281 gatttggcgg tggtgaatac ccctgccggg gaagcgagct ggtatgtgaa tgctatcgag
     5341 tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat ggctggcatt tttgccagcc
     5401 agtccgtata actatcccct gatttttgat ccgaaaagat gaactcaaac catgatgaat
     5461 gattcgttct gccgcattat tgcgggtgaa attcaggcgc gcccggaaca ggttgacgct
     5521 gccgttcgcc tgcttgacga agggaatacc gtgccgttta tcgcacgtta tcgtaaggaa
     5581 atcaccggcg gtctggatga cacgcagctg cgtaatctgg aaacgcgtct gagctatctg
     5641 cgcgagctgg aagagagacg tcaggcgatc ctcaagtcca tttccgagca aggcaaactc
     5701 actgacgagc tggcgaatgc catcaacgcc acactaagca aaaccgaact cgaagacctc
     5761 tacctgccct acaaacctaa acgccgcacc cgcgggcaaa tcgccattga agcagggctt
     5821 gagccgttgg ctgacctgct gtggagcgat ccgtcacaca cgccagaagt cgccgctgca
     5881 caatatattg atgccgataa aggcgtggca gataccaaag ccgcgctgga cggcgcgcgc
     5941 tatatcctaa tggaacggtt tgccgaagat gccgcgctgc tggcgaaagt gcgtgattat
     6001 ctgtggaaga acgcgcattt ggtctcaacc gtggtgaatg gtaaagaaga ggaaggggcg
     6061 aaattccgcg actatttcga tcatcacgaa ccgctgtcca cggtgccttc tcaccgcgcg
     6121 ctggcgatgt tccgtggtcg taacgaaggc gtacttcagc tttcgctgaa tgccgatccg
     6181 cagttcgaag agccgccgaa agagagctat tgcgagcaaa tcatcatgga tcaccttggc
     6241 ctgcgcctga acaatgcccc ggcggatagc tggcgcaaag gtgtggtgag ctggacgtgg
     6301 cgcatcaagg tgttgatgca tctggaaact gaactgatgg gcaccgtgcg cgaacgcgcg
     6361 gaagatgaag caatcaacgt ctttgcccgt aacctgcacg atctgctgat ggcggcccct
     6421 gccggactgc gtgcaacgat gggcctcgat ccgggtctgc gtaccggggt aaaagtggcg
     6481 gtagtcgatg cgacaggtaa actggtggcg accgatacca tttatccgca taccgggcag
     6541 gccgcaaaag cagcgatgac cgttgctgcg ctgtgtgaaa agcataacgt tgaactggtg
     6601 gcgatcggca acggtacagc ttctcgcgaa accgaacgtt tctatctcga cgtgcagaag
     6661 cagttcccga aagtgaccgc gcagaaagtg atcgtcagcg aagctggcgc gtcggtttac
     6721 tcggcttccg agctggcagc acaggaattc ccggatctcg acgtttcgct gcgtggcgcg
     6781 gtgtctatcg cccgtcgttt gcaggatccg ctggcggagc tggtgaaaat cgatccgaaa
     6841 tctatcggcg taggtcagta tcagcatgac gtcagccaga cgcagctggc acgcaaactg
     6901 gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg atctcaacac cgcttctgtt
     6961 cctctgttaa cccgcgtggc gggcctgacg cgcatgatgg cgcaaaacat cgtggcctgg
     7021 cgcgatgaga acggccagtt ccagaaccgt cagcaactgt taaaagtgag ccgtctgggg
     7081 ccgaaagcct tcgagcagtg cgcgggcttc ttgcgcatta accatggtga taacccgctg
     7141 gacgcgtcta ccgtccaccc ggaagcctat ccggtggtgg aacgcattct ggcagcaaca
     7201 cagcaggcac tgaaagatct gatgggtaac agcagcgagc tgcgtaacct gaaagcgtct
     7261 gactttactg atgaaaaatt cggtgtgccg acggtaaccg acatcatcaa agagctggaa
     7321 aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc agtttgccga tggcgtcgag
     7381 acaatgaacg acctgcaacc gggtatgatc ctcgaaggtg cggtgaccaa cgtcaccaac
     7441 tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc tggttcacat ctcttcattg
     7501 tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag cgggcgacat tgtgaaggtg
     7561 aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc tgaccatgcg tctggacgag
     7621 cagcctggcg aaaccaacgc ccgtcgcggt ggcggtaacg atcgcccgca aaacaaccgc
     7681 ccggcagcca aaccgcgtgg gcgtgaagcg cagcctgccg gtaatagcgc gatgatggat
     7741 gcgctggcgg cggcaatggg caaaaaacgt taaacgcccg gactggcccc taaacctgcg
     7801 cgattaggcg ttcacgccac atctggcaac gaacgccttg cctgacataa aagtgccgga
     7861 gaatatctcc ggccttttta ttccacaagc aaactcataa tatatcccat taatatttat
     7921 cacttcatta acaactgaaa ccttaattaa acattaacca gccctaataa ttcgtcatgc
     7981 gaattatatt ttcgctgcga tataaccttg agccatatca acattgagtc agattattat
     8041 tcaaaccaac attcacacac attttaagta ttgctgatag aaaccattct cattatcatt
     8101 gtactgttga ttatttaatc tctccttcgt tggcaaatca tctggtctca tgtcgctgtc
     8161 aaacgcccca tgaggtagtt atccagttaa tgagaaacaa gtaggcacct atgcaataca
     8221 ctccagatac tgcgtggaaa attactggct tttcccgtga aatcagcccg gcatatcgcc
     8281 aaaaactgct ttctcttggc atgttacctg gctcctcttt taatgtggtg cgcgtcgctc
     8341 cactcggcga ccccattcat atcgaaaccc gtcgtgtgag cctggtatta cgaaaaaaag
     8401 atctggcctt attagaagtg gaagcggttt cctgttaata cggtgataac aacaatgaaa
     8461 aaattaacca ttggcttaat tggtaatcca aattctggca agacaacgtt atttaaccag
     8521 ctcactggct cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga acgtaaagaa
     8581 gggcaattct ccaccaccga tcatcaggtc acgctggtgg acctgcccgg cacctattct
     8641 ctgaccacca tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca ctacattttg
     8701 agtggcgacg cagacctgct gattaacgtg gtggatgcgt ctaaccttga gcgtaacctg
     8761 tacctgacgc tgcaactgtt ggaactcggc attccctgca tcgtggcact gaacatgctc
     8821 gacattgccg agaagcaaaa tattcgtatt gaaattgatg ctctgtcggc gcgtcttggc
     8881 tgcccggtga tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct caagctggcg
     8941 attgatcgct ataaagctaa cgagaatgtg gaactggtgc attacgcaca gccgctactc
     9001 aacgaagcag attcactggc aaaagtgatg ccttccgaca tcccgctgaa acaacgtcgc
     9061 tggctgggcc tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc cggtgaagcg
     9121 tcgcagcatc tggatgccgc cctcgcccgt ctgcgtaatg agatggacga tccggcgctg
     9181 cacattgccg atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt aagcaacacc
     9241 ctgacggcag aacccagccg tttcaccact gcggtagata aaatcgtgct caaccgtttc
     9301 ctcggtctgc cgattttcct ctttgtgatg tacctgatgt tcctgctggc tatcaacatc
     9361 ggcggggcgt tacagccgct gtttgatgtc ggttccgtgg cgctgtttgt gcatggtatt
     9421 cagtggattg gctacacgct ccacttcccg gactggctga ctatcttcct cgcccagggc
     9481 ctgggtggcg gtattaacac cgtgctgcca ctggtgccgc agatcggcat gatgtacctg
     9541 ttcctctcct tccttgagga ctccggctat atggcgcgtg cggcgtttgt gatggaccgc
     9601 ctgatgcagg cgctgggctt gccggggaaa tcctttgtac cgctgatcgt cggcttcggt
     9661 tgtaacgtac catcggtaat gggcgcacgt acgcttgatg caccgcgtga acgcctgatg
     9721 accatcatga tggcaccgtt tatgtcctgc ggcgcacgtc tggctatctt cgcagtattc
     9781 gcggctgcct tcttcgggca gaacggtgcg ctggcggtct tctcgctgta tatgctgggt
     9841 atcgtaatgg cggtgctgac gggcctgatg ctcaagtaca ccatcatgcg cggtgaagcg
     9901 acgccgtttg tcatggagct gccggtctac catgtaccac acgttaaaag cctgattatc
     9961 cagacctggc agcgtctgaa aggcttcgtt ctgcgtgctg gtaaagtgat tatcatcgtc
    10021 agcattttcc tgagcgcctt caacagcttc tcgctgagcg gaaaaatcgt cgataacatc
    10081 aacgactcgg cgctggcttc cgtcagccgg gtgatcaccc cagtcttcaa gccgattggc
    10141 gtgcatgaag ataactggca ggcaacggtt ggcctgttta caggcgcaat ggcgaaagaa
    10201 gtggtggtgg gcacgctcaa caccctctac accgcagaaa atattcagga cgaagagttc
    10261 aatccggcag aatttaacct cggtgaagag ctgttcagcg cggtagatga aacctggcag
    10321 agcctgaaag acaccttcag ccttagcgta ctgatgaatc ccattgaagc gagcaaaggc
    10381 gacggcgaaa tggggaccgg ggcgatgggc gtgatggatc agaaattcgg tagcgcagct
    10441 gccgcataca gctacctgat tttcgtcctg ctgtatgtac cgtgcatctc ggtgatgggg
    10501 gctatcgccc gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg ggggctgaat
    10561 atcgcttact cactggcaac attgttctat caggtcgcca gctacagcca gcacccaact
    10621 tacagcctgg tgtgcattct ggcggttatc ctgtttaaca tcgtggttat cggtttgctg
    10681 cgtcgcgcgc gtagccgggt ggatatcgaa ctgctggcaa cgcgcaagtc ggtaagcagt
    10741 tgctgcgcag ccagcaccac cggtgattgc cattaatggc ttcacttatt caggtgcgtg
    10801 atttgctggc gttacggggc cgtatggaag cggcccagat aagccagaca ttgaacaccc
    10861 cgcagccaat gattaacgcc atgctgcaac agctggaaag catgggcaaa gccgtgcgga
    10921 ttcaggaaga acctgacggc tgcctctctg gcaattgtaa aagctgcccg gaaggcaaag
    10981 cctgcctgcg cgagtggtgg gcgctgcgtt aaccatactc catcacctgt tttgaaaagc
    11041 ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt tccataaaaa tgcaaccctt
    11101 gcagcgcgac gtaaattcct ctgaaagcgt ctcgcaggga tgaaaactcg ctaatgcaca
    11161 ggtgtggagt ggtgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa cgccatgagc
    11221 aaaaagcaga gttccacccc acacgatgcg ctgttcaaac tctttttacg ccaaccggag
    11281 acggcgcgcg attttcttgc gtttcattta ccggcaccta ttcacgcgct ctgtgatatg
    11341 aaaaccctca agctggagtc gagcagcttt attgatgacg atctgcgtga aagctattcc
    11401 gacgtgctgt ggtcggtgaa aacggaacaa gggccaggat atatctattg tctgattgaa
    11461 catcaaagca cctcaaacaa actgatcgca tttcgcatga tgcgttacgc cattgccgca
    11521 atgcaaaatc acctggatgc cggatacaaa acattgccga tggtggtgcc attgttgttt
    11581 taccacggta ttgaaagccc ctatccctat tcgctgtgtt ggcttgattg tttcgccgat
    11641 cccaaactgg caaggcagct ttatgcctcc gcatttccgc tgattgatgt caccgtcatg
    11701 cctgatgatg aaatcatgca gcaccgacgt atggcgctgc tggagttaat tcaaaaacat
    11761 attcgtcaac gcgatctgat ggggctggta gagcaaatgg cctgcttatt aagtagtgga
    11821 tacgctaatg acagacaaat caaagggctg tttaattaca tcttgcaaac cggcgatgcg
    11881 gtacgtttta acgattttat cgacggcgtt gccgaacatt caccgaaaca caaggagagc
    11941 ttaatgacta ttgcggaaag attgcggcaa gaggggtttc agaaaggtat acgagctatc
    12001 gccaaaacca tgctggatgc cggagtccct cttgaggacg ttctgcgatt taccagcctg
    12061 acggttgaag accttgctga aataaatcat caataacgta aaggatggtt ttaatgacag
    12121 atgcagaaag agaatatctt attagttacc tgattggtta tttattgggt cgacgcctgg
    12181 aagccctgga tatcgccaaa agaatgctgt aatccggagt ccctcttgca gacatcatgc
    12241 gctttaccgg ggtgtcagaa gaagagttgg ctgcggcgag ccagtaaagt tctgtctcgc
    12301 catttcaaaa gccacctaca ccctctgctt caacgccacc agcatgcgac aaaactcggc
    12361 cggatgcgaa ataaacggcg catgggccgc tttggcgaag atgtatgatt cgctgtgagg
    12421 ccaaagttta tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata
    12481 caatcgcaaa aacggcatgg acacgttttg cagcggctga cggagatcga ccattttcag
    12541 gatttccagc ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt
    12601 cttcagcgcc cgcgcatcct ggcgcgccgt ttcagtaccc atggtttgta acgccaggaa
    12661 ccgctccacc gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagcacgtc
    12721 cggttttatc cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac
    12781 cagcgcctga acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag
    12841 actccagcct aaccaaatgg ctttatcagg tgcctgtcgc agcacggctt cggccatatc
    12901 agcaagtgac atcgcaccaa atccccggct acgcccgaag ccgggcagat caacaaggtg
    12961 cagcgtaaaa tgcgagctca gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc
    13021 ccatccgtgc agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt
    13081 attcatccgc tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg
    13141 attatgctgg ctatgccgaa tgccgctggc gttaggtcat tgggggattt gttcggtatg
    13201 ctcacgcgcc gcccgtacag ataaaacgtt atgcccccaa tgtggattac ccgccacaca
    13261 ctcacatctt ccctgcggtc gctgcctgca aaaaccgccg ccctggcaaa gactggtcac
    13321 ggttgccgac tatgcgccgc cgttaagtcc gcttatccac cagcttaagt tttcccggcg
    13381 cagcgaaatc gccagcgccc tggcacgtct gttactattg gaagtcttac acgctcgtcg
    13441 caccaccggg ttgcaattgc cggatcgcat tatcagcgtt ccgttatggc agcggcgtca
    13501 ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca
    13561 ctgccgatgg gatagcgaag ccgtcacacg aacacgggcc actgcgaccc agcattttct
    13621 cagtgcccgg ctgcgcaagc gcaacctgaa aaatgccttt cgccttgaat tgcctgtgca
    13681 aggtcgccat atggtgattg tggatgatgt cgtcaccacc ggaagtaccg tcgcagagat
    13741 tgcgcagttg cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac
    13801 cttgtagagc ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca
    13861 ttactatgat ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc
    13921 aggaagaagg gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat
    13981 gtggcgtttc ttattgtccg ccggacgctg tagaagccac cgacactgcc ctgaaatttg
    14041 acctgctgac cgcgtatgtt gatgagttaa gcgctcctta cctggaagat gcagagatcg
    14101 actttgttac agaccagttg ggttcccagt taacgctgaa agcgcctaac gccaaaatgc
    14161 gtaaagtcgc agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga
    14221 tcaacccaca gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg
    14281 gttacgccat tctgcaattt ggcggcggct gtaacggttg ttctatggta gatgtgacgc
    14341 tgaaagaagg gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg
    14401 atctcaccga acaccagcgc ggcgagcact cctactacta agttatcccc tcttttgtag
    14461 attgcccgat gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt
    14521 ggcgcagcat tgctttggca aacccctccc ttcgtttccc cgcgataata tgaccaacct
    14581 ctcataattt aaatttaccc cgctctggtg attctcaaac gccggatgtt acccgtatca
    14641 ttcacgtggg taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta
    14701 tcagtcgtgg tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga
    14761 aaattgacgt tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg
    14821 ctatcggtgt aatcttgttg ttgctcctga tgatccgttt caaaatgaac ggcttcatcg
    14881 ctctcgtcct cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta
    14941 ttggctccat caaagcaggt gtcggcggaa cgctcggtag ccttgccctg atcatgggtt
    15001 ttggcgcaat gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca
    15061 cgctgattgc taaatttggt aaaaaacaca tccagtgggc ggtggtactg accggtttta
    15121 ccgttggttt tgccctgttc tatgaagtgg gctttgtgct gatgctgccg ctggtgttta
    15181 ccatcgcggc ttccgcgaat attccactgc tgtatgttgg tgtaccaatg gcggctgcac
    15241 tgtctgtgac ccacggcttc ctgccaccgc atccgggccc gactgcgatt gccaccattt
    15301 tcaatgccga tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga
    15361 ttctcgccgg tccggtttac gctcgcgtgc tgaaaggtat agataagccg atcccggaag
    15421 gtctgtacag cgcgaaaacc ttcagcgaag aagagatgcc aagttttggc gtcagcgtct
    15481 ggacctctct ggtcccggtt gtgctgatgg cgatgcgtgc gattgccgaa atgatcctgc
    15541 cgaaaggtca cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggctacgc
    15601 tgattgccgt actgattgcg atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc
    15661 agattaacga cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgattatcg
    15721 gtggtggcgg tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt
    15781 ccatgatgca cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac
    15841 tgcgtatcgc gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac
    15901 cgctgattgc aacgacgggt gttagcccgg agctgatggt tattgcggtt ggttccggta
    15961 gtgtgatttt ctctcacgtg aacgatccgg gcttctggct gttcaaagag tactttaacc
    16021 tgactatcgg cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg
    16081 gtctggtagg ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat
    16141 cgcatccggc atcaaccgca ctctacttct tcttcgctgc cgctctgcgc cgtctgtcca
    16201 aatccttcag caacttgttc acaccatcat cggcaaacat cgcctcaagc gttgcggaga
    16261 gcttgcgccg ccagttttta tactggtaac tggtgccggg aatatttacc gggtccgcca
    16321 tatccagcca gtcttctggc tgtagtccta acagggcgct gttgctatcg gcgatgtagc
    16381 gctgtaagcc acgattcagc gtcggtgtca tcgacatcaa cgatgcctta tgcccggcac
    16441 gttttggcag gcaaccatat ttatgcagag catccagtag tccttgcttc gccagttcgc
    16501 gatcctgata cagaccgcgc agtaccactt catccggata cagccccagc gttttgccca
    16561 gcgttaaatc cccgctctcc cagtagccgc gtagcgtagg caggtcgtgt gtcgccgcaa
    16621 ccgccatcga ctgctctggg tacgctttcg gtgcgcggaa tgtcttctcg tggtcgtttt
    16681 cgaaatagag cactttgtag gagtacacac cgctgctgcg cagcttaccg acaatctcta
    16741 ccggtacggt accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg
    16801 ccagaatcga gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct
    16861 cgccatacgg tatccaccac aaacgcagca tcgacatcac gtgatcaatt cgtaatgcgc
    16921 cgcagttttg catattggca cgcaacagct cgataaacgg ttcataggca cgcgcggtga
    16981 taatatgcgg gtccattggc ggcaatcccc agttctgccc caacgggccg aggatatccg
    17041 gcggtgctcc aaccgacgct ttcaggcaat acaactcccg gtcgcaccag gtttctgatc
    17101 caccctctgc tacaccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct
    17161 ggcttatctc ccagcaggcg gcaaactggc tgtaagccag ccactgcaac cagagataaa
    17221 aatcgacgtc atcacgatgt tcttcgcaga actggcgcac ttctggtgaa tccacgttct
    17281 gatacatctc tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg
    17341 catgtagcgc gtcaaacgcc gcctgccaga acaggctatc gccttgctcg gcaacaaact
    17401 gacgaaacgc ggccacctgt tcatcatcac gttgcgcgaa acctttccac gccatgcgca
    17461 atgctgtcat ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcccgcgtct
    17521 gttgcagcgt ctgttgcgtg gtcggcaact tccaccaggc ctgagcctct tcgctaagat
    17581 ggaaatcttc gacggcgtta acgtcgatat aaatcacatt cagccaacgg cgagaagacg
    17641 ggctgtatgg gctggcactt tccggatttg ccggatagag cgcatgaatc gggttcagac
    17701 cgatgaacga cccgccacgt tttgccacat ccaccagcat cgctttgaga tcgccaaaat
    17761 ccccaatacc ccagtttttt tccgatcgca gcgtataaag ctgaacgcag gcaccccaca
    17821 gcttttgttt attcagcaac gcctgcggtt cgtaacagcg tttcggggcg acaatcaccc
    17881 tgcaatgcac gcgctggtct tcctgggtaa acgtcagcgt gtgataacct tccggcagct
    17941 tcgtcggtag attgaacgct ttgccccccg ttacatggcc tttgtactgc gttccttctt
    18001 cggtggtcag cagccagcta tattcgccgc tgccctccac caccatcggc atttttttgc
    18061 cgctggtata aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt
    18121 gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt
    18181 gggcattgat gtaattgggg ctaatccccg ccgccagcgc ggcattatcc agacgtttgc
    18241 tttccatcga gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag
    18301 agcgatccga gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg
    18361 cctcctggtc gcggtacagc acatccacct gcttttgtgc ctctacatag gctgcgaaat
    18421 ccgccatcac cagatacgga tcgccgccct gtttgccgat actgtgcagc atctgatcga
    18481 aggcatgctt atcgccgtcg ctgtatttgc cgctttccag ctctttcaat actgcgtcca
    18541 gcaccttatc tttcttccgc catttcaccg ggtcgtagcc tttggccaga attgccttca
    18601 cttgttccac ggtatgacca aaaataaaga tattttcttc accaactttc tcggcgattt
    18661 caacgttcgc accatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat
    18721 tgccggtacc ggaagcttct ttacctgccg tcgaaatttg ttcggagata tccgccgccg
    18781 ggatcagttt ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaatttgt
    18841 cgccaacctg cggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatgt
    18901 ttttcgccag gtagtagccc ggtgccgctt tcgcgccaaa gaggaagacg cgcggtacgc
    18961 gatcagcctg cgggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca
    19021 ggtgctggcg tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat
    19081 tgatctcaat accggtacga actttcacaa actctgccag acggacttta ttcgcctgct
    19141 tgatctcgcg atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca
    19201 gttgatcgag atcgttagtc cactcttttt tcagtgattt atccaacaga gccgccagtg
    19261 ccgggttgca ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg ttatggaatt
    19321 tgttcggcca tagctggtga tattccggga acagatcttt caccaccaga tccgagtgca
    19381 gggccgcaac accgtttacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt
    19441 gtttgtcgtg taccaccgcc agtttggccc atactttttc atcgcccggc caggtttttt
    19501 ccaccagcgt tttaaagcgt gtattaattt cgttaataat ctgcatgtgg cgcggcagta
    19561 agcctttcac cagtttcaca tcccagcgtt ccagcgcttc tggcatcaag gtatggttgg
    19621 tgtaagcgaa agttttactg gtgatggccc aggcatcatc ccagctcatc tggtgctcat
    19681 cgatcagcac gcgcagcagt tctggaatcg cgatagttgg gtgggtatcg ttcagctgaa
    19741 taacttcgta ctccgccagc tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa
    19801 tatccgctac cgagcaggca cactggaagt attgctgcat caggcgcagc tttttaccgg
    19861 cagtatggtt gtcgtttgga tagagaactt tggtcagttt ttccgcgttg atgccctgct
    19921 gttcggcacg caggaaatca ccatcgttaa atttagtcag atcaaacgga tgcgcatgcg
    19981 tcgcctgcca cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat
    20041 cccacgcttg accggtaatg gtaaactccg gctcccagcg tccgtctttc gtcactttac
    20101 cgccaatccc tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc
    20161 gatgccagtc atccggcgct tcaacctgtt tgccatcgac aaatgactgg cggaacaagc
    20221 catattgata attaaggccg taaccagtag ctgactgccc gacagttgcc attgagtcga
    20281 ggaagcacgc cgccagacgt cccagaccac cgttacccag cgccgggtcg atctcttctt
    20341 ccagcaagtc agtcaggttg atgtcataag ccttcaacga atcctgtaca tcctgatacc
    20401 agccgagatt caacagattg ttgcccgtca ggcgaccaat caaaaactcc attgagatgt
    20461 agttaacatg ccgctgattc gccaccggtt tggcaaatgg ctgagcacgc agcatttcgg
    20521 ccagtgcttc gctcactgcc aaccaccact gacgaggggt catttcagcc gcagaattta
    20581 agccataacg ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag
    20641 gttgtgacat aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc
    20701 caggctcgat gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga
    20761 tgagcaagga aatgtgatct cgaccactta aagctagtgc aaaccacagg attagcagca
    20821 aatcaatgcc atacagcgca gaaaatctgt atcaaagtgc aaaaaatggc cgttgcgtat
    20881 tttcaaaaag cggaaggtaa ctctataatt aagtaaagga gtgaaacagt ttcacaagta
    20941 aaatatccag tgtgctccat ctcattctta atagatttat taagatcatc tttttagatg
    21001 gcactttcat cagaaatgaa gaagaaaccc ttgcttaaat gaaactgatg aacataagga
    21061 aaataccgta caacccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata
    21121 aacatttgga attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca
    21181 ttagaaaggt ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat
    21241 ttcctcattt tccacagtga agtgattaac tatgctgatt ccgtcaaaat taagtcgccc
    21301 ggttcgactc gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa
    21361 caacttccgg ctggcgctga tcacaagtcc tgcgggctac ggaaagacca cgctcatttc
    21421 ccagtgggcg gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag gtgataacca
    21481 gcaagagcgt ttcgccagct atctcattgc cgccgtgcaa caggcaacca atggtcactg
    21541 cgcgatatgt gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc
    21601 ccagcttttc attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta
    21661 tcatctgatc actaatcctg tgatccacga gtcaatgcgc ttctttattc gccatcaacc
    21721 agaaaatctc acccttgtgg tgttgtcacg caaccttccg caactgggca ttgccaatct
    21781 gcgtgttcgt gaccagctgc tggaaattgg cagtcagcaa ctggcattta cccatcagga
    21841 agcgaagcag ttttttgatt gccgtctgtc atcgccaatt gaagccgcag aaagcagtcg
    21901 gatttgcgat gacgtttccg gttgggcgac ggcactgcag ctaatcgccc tctccgcccg
    21961 gcagaatact cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca
    22021 tctttcggat tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt
    22081 tctgttgaaa agcgccattt tgcgctcaat gaacgatgca ctcatcaccc gtgtgaccgg
    22141 cgaagaaaac gggcaaatgc gcctcgaaga gattgagcgt caggggctgt ttttacagcg
    22201 gatggatgat accggcgatt ggttttgcta tcacccactg tttggtaact tcctgcgcca
    22261 gcgctgccag tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag
    22321 ctggatggcc cagggatttc ccagcgaagc gattcatcat gcgctggcgg caggcgatgc
    22381 gctgatgctg cgcgatattc tgcttaatca cgcctggagt ttgttcaacc atagcgaact
    22441 gtcgctgctg gaagagtcgc ttaaggccct gccgtgggac agtttgctgg aaaatccgca
    22501 gttggtgtta ttgcaggcgt ggctgatgca aagccagcat cgctacggcg aagttaacac
    22561 cctactggcc cgcgctgaac atgaaatcaa ggacattaga gaaggcacca tgcacgcaga
    22621 atttaacgct ctgcgcgccc aggtagcgat taacgatggt aatccggatg aagcggaacg
    22681 gctggcaaaa ctggcactgg aagagctgcc gccgggctgg ttctatagcc gcattgtggc
    22741 aacctcggtg ctgggtgaag tgctgcactg caaaggcgaa ttgacccgct cactggcgct
    22801 aatgcagcaa accgaacaga tggcacgcca gcacgacgtc tggcactacg ccttatggag
    22861 tttaatccag caaagtgaaa ttctgtttgc tcagggattc ctgcaaaccg cgtgggaaac
    22921 gcaggaaaaa gcgttccagc tgatcaacga gcagcatctg gaacagctgc caatgcatga
    22981 gtttctggtg cgcatccgag cacaactgtt atgggcctgg gcacggctgg atgaagccga
    23041 agcgtcggcg cgtagcggga ttgaagtctt gtcgtcttat cagccacagc aacagcttca
    23101 gtgcctggca atgttgattc aatgctcgct ggcccgtggt gatttagata acgctcgtag
    23161 ccagcttaac cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc
    23221 taacgccaac aaagtccggg tgatttactg gcaaatgacc agcgataaag ccgccgctgc
    23281 caactggttg cgtcatacgg ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca
    23341 atggcacaac attgcccgtg cacaaatctt gctgggcgag tttgaaccgg cagaaattgt
    23401 tctcgaagaa ctcaatgaaa acgcccgcag tctgcggttg atgagcgatc tcaaccgtaa
    23461 cctgttgctg cttaatcaac tgtactggca ggccggacgt aaaagtgacg cccagcgtgt
    23521 gttgctggac gcattaaaac tggcgaatcg caccggattt atcagtcatt ttgtcatcga
    23581 aggcgaagcg atggcgcaac aactgcgcca gcttattcaa ctcaatacgc tgccggagct
    23641 ggaacagcat cgcgcacagc gtattctgcg agaaatcaat caacatcatc ggcataaatt
    23701 cgcccatttc gatgagaatt tcgttgaacg tctgctaaat catccggaag tgccagaact
    23761 gatccgcacc agcccgttga cccaacgtga atggcaggtc ctggggctga tttactccgg
    23821 ttacagcaac gagcaaattg caggtgagct ggaagtggcg gcaaccacca tcaagacgca
    23881 tatccgcaat ctgtaccaaa aactcggtgt cacccaccgt caggccgcgg tacaacacgc
    23941 gcagaaactg ctgaagatga tgggatatgg ggtgtgagtt tagccggata atacgtcgga
    24001 tccggcttac atctttatat cattcaatgc tcacccgcgt tacgccatcc gcctccacca
    24061 gactaaaccg cactggcaag aaacgctcca ccaccgcaat attggtcagc agatggcatg
    24121 agggatgggc gaccgtaaat tctcccgcgc ccgccagcgc catcggcagt accagctggt
    24181 cggcgagata ttcccccacc gccgccggac ttgccaggta gcgtttcacc tctttcacca
    24241 actgtgccgc gaccacttcg gcgctgacgc gcttttcacc gacgacaaaa aagcgttcgg
    24301 tgatattttc actttcgact tcaagcgaaa cggtattacc cggtccctgg tcacgcggca
    24361 ggttatgaat attctgctca tgcagggaaa aactccccgc cagtgtagcg atttcacgct
    24421 cagcgacatg gcgcggcacg ccagctaata gcacctctcc acgcatctgc acaatgttcc
    24481 cgcgctcgcc aagttgcaag gtgttaaaca atgccaccgg cgagacttcc gttgccacca
    24541 ccccgcctcc agcaggataa aaaccgtggc gtaataatgt ggtttgctga tgaatgccca
    24601 ttttcgccag cagcggctcc agcacccggc ggataaaatc cgcaggcggt gccgacgggt
    24661 tatcggtgcc gccgctcact tcaacacgcg aaggcccatc agcaaaccac agcgcgggta
    24721 gcaccgtttg cagcaccagc gtgctgcttc cggcactacc gatagcaaag cggtaatcgc
    24781 cgccacgcac ggtaccgggc cggaagacca gacgctgtga ccccagctcc gcgccttcca
    24841 ccgttgccct acaaatttcc gcagccgctt ttaccgcggt cagatgttgg cgcaacagcc
    24901 ccggtttcgc ccgcccggca cgaatgccgg tgatggtaaa tggctggccg gttatcatcg
    24961 acaggctcag cgccgagcgc aggatctgcc cgccgccttc gccctgtgcg ccatccagcg
    25021 caatcatcct tttcatcatt tatcctttta cgcacaccac ctgacgcagg gtatagataa
    25081 cttccaccag atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcggga
    25141 tttcgtcgat cacttcggca tctttacggc attccacatg cgcggtggca cgaatttgat
    25201 cttccacgct gaacattttt ttcgctttag tacggctcat aacccgcccg gcaccgtggc
    25261 tgcacgaaca gaacgactct tcatttccca gcccacggac gataaagctt ttcgctccca
    25321 tcgaccctgg aataattcca tattgaccag cacgcgcaga caccgcgcct ttacgcgtca
    25381 cgtagatctc ttcaccaaag tgctgttctt tttgcacata gttgtggtga cagttgatct
    25441 cttccatcgc cagcgtttgt ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg
    25501 ttaccacgtt ttccatcatc gcatcgcggt taaggctggc aaaaagctgc gcccaggcca
    25561 cggctttcag gtaatcatca aagtattccg taccttccat aaagtacgcc agatcacgcg
    25621 acggcaatgt ttcaagcgtt tcctgcatct ctttttgcgc cagatcgata aagtaagtcc
    25681 cgatggcgtt accaattccg cgtgaaccgg agtgcagcat aatccacacc tggtccgact
    25741 catcaaggca gatttcaata aagtggttac ccgttcccag cgttcccagg tgtttatagt
    25801 tgttagtgtt aaggaaacgg ggatattttt gcgttaacca ctgataaccg gcttccagct
    25861 cagcccattt cgcatcgacg ttaacaggcg gattttccca tgcgccttta tcacgtttac
    25921 aacgtccggt agtacgtccg tgcggcacgg ctgtttcaat cgcctgacgc agctccgcca
    25981 ggttttccgg caggtctgcc gccgttaacg cggtacgcag cgcgttcatt ccacaaccga
    26041 tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg ctaccaatgg
    26101 tggaaccttt acccaggtgt acatccggca ttaccgcaat atgtttgaaa ataaacggca
    26161 tcttcgccgt attaataagt tgctgacgcg catcggcctc taccggcacg cctttggtcc
    26221 acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg tttttctctt
    26281 ttcgttgttt gctgtcctga taattgcaac cgtcgtgcca gaaaattaaa taagcagctt
    26341 aattttttta tttattgttt ttaaaaagat tatatcttta cgtccgtaac cggagatttc
    26401 ccgcaaagcc gatttaccga taatgaaatc tcgtctttta taaggatatc taagatgcgt
    26461 aaaacagtgg cttttggctt tgtcggaacg gtgctggatt atgccgggcg cggcagtcag
    26521 cgctggtcga aatggcgtcc gtcactctgt ctatgccagc aagaatcgtt agtcatcaat
    26581 cgactggaat tgttgcacga cgcccgctcg cgctcgctgt ttgaaacgct taaatgcgat
    26641 atcgccagcg tttcgccaga aactgaagtg gtgagcgttg agattgaact gcataacccg
    26701 tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggcta cgcgtttcaa
    26761 cctgataaag aagactattt aattcacatc accactggca cccacgtcgc acagatttgc
    26821 tggtttctgc tggcggaagc gcgttacctg cccgcccggc tgatacaatc ttccccaccg
    26881 cgcaaaaaag aacagccgcg cggcgcaggt gaagtgacga ttatcgatct cgatttaagc
    26941 cgttataacg ccatcgccag ccgctttgcc gaggaacggc agcaaacgct cgattttctt
    27001 aagtccggca ttgccacgcg taacccccac ttcaaccgca tgattgagca gattgaaaaa
    27061 gtggcgatta aatcccgcgc accgatcctg cttaacggcc cgacaggtgc gggcaagtca
    27121 tttctggcac gacgcatctt cgaattaaaa caggcacggc atcagtttag cggcgcattt
    27181 gtagaaatga actgcgccac tctgcgtggc gataccgcca tgtcgacgct atttggtcat
    27241 gtgaaaggcg cgtttaccgg ggcgcgggag tcgcgggaag gattattacg cagcgccaac
    27301 ggcgggatgt tatttcttga tgagattggc gaactgggcg cagacgaaca ggcaatgctg
    27361 ctgaaagcca ttgaagagaa aaccttttat ccgtttggca gcgatcgcca ggtgagtagc
    27421 gactttcagc ttatcgccgg aacggtgcgc gatttgcgtc agctggttgc cgaaggcaaa
    27481 ttccgcgaag atctgtacgc gcggatcaat ctctggacct tcaccttgcc ggggctgagc
    27541 cagcgccagg aagatattga accgaacctt gattatgaag tggagcgcca cgcctcactc
    27601 actggcgaca gcgtgcgttt taacaccgaa gcgcggcgcg cctggctggc ttttgcgacc
    27661 tctccccagg caacatggcg cggtaacttt cgcgaacttt ctgccagcgt cacgcggatg
    27721 gcaacctttg ccactagcgg acgcatcact cgggaagtgg tggaagatga gataaaccgt
    27781 ctgcgctata actggcagga gagtcgcccc tccgcgatta cggcgttgct gggcgcagag
    27841 gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgtaatagc catctgccgc
    27901 caggcaaagt cactttctgc tgccggtcgc cagctttttg atgtttcgcg ccagggcaaa
    27961 gccagcgtca acgacgcaga tcggctacgc aaatacctgg cgcgttttgg tctgacgtgg
    28021 gaagccgtgc aggatcagca cagctccagt tgaatatgat ggtccgtcag cacctgcatg
    28081 acgcttactg gcggcggggc gtcggtgtag acagcatcaa ccatgctgat gctccccata
    28141 ttgaccatcg cattacggcc aaatttcgag tgatcgacaa ccagcataac gtggcgcgag
    28201 ttctcaataa tggcgcgttt ggtgcgaact tcgtggtaat cgaattccag caacgagccg
    28261 tcgctatcga tgccgctgat ccccagaatg ccgaaatcaa ggcggaactg ggagataaaa
    28321 tcgagcgtcg cttcgcctat gatcccacca tcgcggctac gtaattcgcc accggcgaga
    28381 ataatgcgaa aatcctcttt taccatcagc gtgttagcga cgttaagatt gttggtgaca
    28441 atgcgcaaat tgctgtgatt gagcagcgcg tgcgccaccg cttccggggt ggtgccgata
    28501 tcgataaaca gcgttgagcc attaggaatt tgctccgcca ctttgcgggc gatgcgctct
    28561 ttttcttcgg tctgggtggc tttacgatcg tgccatggcg tgttaaccga actggaaggc
    28621 agcgccgcac cgccatgatg gcgcaggatc aggttttgct ctgccagctc gttgaggtcg
    28681 cgacgaatag tctgcgggct gacggagaag tgctccacca gttcctcggt actgacataa
    28741 ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt catttataaa
    28801 tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca tcgctaaacc
    28861 cacggctaac ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca aatcaaacca
    28921 tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt aaatgccact
    28981 ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca ccacgccaga
    29041 aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta acagggcgct
    29101 aatgagagta atgacaatta gcttaccgct accgaggcgt ttttccaccg caccgccgag
    29161 ataccaccac cagagcaggt taaagaggat atgcatcagc gagaagtgca ttaacgcgtg
    29221 ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg ccagccataa
    29281 catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc aggcgatcat
    29341 catcacccag gttaccggac ctgcgcgttc acgcaaggca gcaaagaaag gataacggcg
    29401 ataatgcagg ccactgccgg tatggcccgc ctgccagctg gccgccagat aacgcggatc
    29461 tgccgggttt tcgagaaaac gcgccagctc tgcccgcacg cgctcggcct gggactcatc
    29521 cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac cctgcgtcgc
    29581 catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa tcatcaacat
    29641 cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc gccgtacgcc
    29701 acctctgcag gaaactgacg ccgccaggct tcaaagccgc cgtcaatgct atagaccaca
    29761 tcgtagccct gttgcagcag atactgcgcc gcgcctttgc tgctattgcc gtgataacac
    29821 atcaccatca ccggtgtgtc aaagtcgtta tcacgcataa acgcgcccag tgtgtcgtta
    29881 gttaaatgga aagcctgcac cgcatgcccc attgcgaaac tctgtggatc gcgaatatcg
    29941 accagcaccg cctctttttc ctgcaacttc tggtgcgcga cggcaacgtt aatacattcg
    30001 aactgctcca tgcgtctctc tttctttaca aacaagtggg caaatttacc gcacagttta
    30061 cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa
    30121 tgttacctaa agcgcgattc tttgctaata tgttcgataa cgaacattta tgagctttaa
    30181 cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt ggcggcatca
    30241 atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg atgctggagg
    30301 cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac ggtggcctgc
    30361 gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa cgtgaagtgc
    30421 tactgaaaat ggccccgcat atcgccttcc cgatgcgttt tcgcctgccg catcgtccgc
    30481 atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat ctgggtaaac
    30541 gcaccagcct gccgggatca accggtttgc gttttggcgc aaattcagtg ttaaaaccgg
    30601 aaattaagcg cggattcgaa tattccgact gttgggtaga cgacgcccgt ctggtactcg
    30661 ccaacgccca gatggtagtg cgtaaaggcg gtgaagtgct tactcgtact cgtgccactt
    30721 ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat accggcaaaa
    30781 aatatagctg gcaagcgcgc ggcctcgtga acgccaccgg cccgtgggta aagcagttct
    30841 tcgacgacgg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa ggcagccata
    30901 ttgtcgtgcc gcgcgttcat acacaaaagc aggcctacat tctgcaaaac gaagataaac
    30961 gtattgtctt cgtgatcccg tggatggacg agttttccat catcggcacc accgatgtgg
    31021 aatacaaagg tgatccaaaa gcagtgaaga ttgaagagag tgaaatcaat tacctgctga
    31081 aagtgtataa cacgcacttt aaaaagcagt taagccggga cgatatcgtc tggacttact
    31141 ccggcgtgcg tccgctgtgt gatgatgagt ccgattcgcc gcaggctatc actcgtgatt
    31201 acacccttga tatccatgat gaaaacggca aagcgccgct gctgtcggta ttcggcggta
    31261 agctgaccac ctaccgcaaa ctggcggagc acgcgctgga aaaactgacg ccatattatc
    31321 agggcattgg tccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc attgaaggcg
    31381 accgcgacga ttatgccgct cgcctgcgcc gccgctatcc gttcctgact gaatcgctgg
    31441 cacgccatta cgcgcgcacg tacggcagca acagcgagtt gctgctcggc aatgcgggga
    31501 cggtaagcga tctcggggaa gatttcggtc atgagttcta cgaagcggag ctgaaatacc
    31561 tggtggatca cgaatgggtc cgccgcgccg acgatgccct gtggcgtcgc acaaaacaag
    31621 gcatgtggct gaatgcggaa caacaatctc gtgtgagtca gtggctggtg gagtatacgc
    31681 agcagaagtt gtcgctggcg tcgtaaggat gatgcccggt tgtgaagtgc aaccgggcta
    31741 tttggattcc ttagactatt cattgtaaat aattgaataa cggcccctga gctaaaatcg
    31801 ccaaacttaa gcacgttccc cactcaaccc acttctgccc ctggcttcta tttttgctac
    31861 gccactggca gtaatatccc aacgcaaaac caagctgtgc actaatcggg ccgacaaaat
    31921 cagtccacgg ctgcggccaa aaaggggaaa aattcagggg aagccagaca aacgtcagaa
    31981 agaagtttcg gatattttcc agcaacgctg atttctctgg agtaatataa agaatgaaat
    32041 tcgagagaat taaccagaaa taggccggag aaaagaccag ccagtaatgc agtctgtttt
    32101 ttaattcagg aggcgttctg cgggagaatt gccaaccgat gccacccatt acactgaaaa
    32161 aaagtgcata ttcaaccatt aactcaagtt gtaattcctg aagagaaata cgcacaacca
    32221 ggagcggaaa ggagaatatt atttcagcca gaaaagttaa cgtgaatata agtacggcta
    32281 aggcaataac cgtcggccat accaataatg gcacgataac aaaaccgaga attacagggg
    32341 gaatataaag ccggaattta atcattttta ttttcctggt gattcggatg atgcgtcata
    32401 catcgcgcat aaacactcat caatcatcca gtcgtcttcc cagacctgat aatgtgtgcc
    32461 ctgttcgggc tgattaagca ggttctcaat cgcctgccgc gcacgttctt tcaggatgaa
    32521 ttgattgttg aaggcccagg cattgatttg ggcggtaatg gctttatcgt catcatcctg
    32581 ctcaaaacga ctattctgaa tattctcttc cctgttatgc cacagcaaac atgccatcga
    32641 tactgtgggt tggtaaccgg gtacagagat gatggcatca agcaatggcg aaagagcgat
    32701 atcggcacgg gaaaacattt ccagcatata ctgtaattcg acagcctcat cgccagatac
    32761 ccaatcagcc atatactggc aggcaaatcg ctgcaatatt gcccgttcat cctcacgcca
    32821 gttttcttta ctggcaaggt tgatacgccg tagagaaaat acttcgtgaa tattgtcaaa
    32881 ttgcccctgc gcaacaaact ccagtaaacg aggaagaaaa taacgaacct catcgctgtt
    32941 ttgcggatca ggtcctggac tactattcca ggcattgatt aaggaataag gaatcgctcg
    33001 caacgatgta ttccgtaatg ctttttgttc ctgttgactc aggcaatatt cacaacaaac
    33061 cacaaattgc tgcggcaccg gataacgacg aaaaatatga taaacctgct caattaatgc
    33121 ctgcatggcg gagctgggtt gcataattac cccctcttcc gctggtcgcg tgtaaacggt
    33181 tgataaacaa aacgagcatt cgccgcattc aggcgaataa tggaactacg agagagggaa
    33241 aagctgggcc gaatgcaata attacaatcc cgacagcttt cctctgtgca attttccact
    33301 gagtaatgac gcgcgtagag tttcagttca gcctgcttat cccgaaaacc gcgtaagctg
    33361 ttatcggtat ggaatttcca tgcttcttta aacacctgaa agatataaac atcctcaatc
    33421 caggagtcac attttttaag cgggcgcttt gctatgatca cccgctcatc cggattgagc
    33481 attgccgtaa aatgaacggt aaagtcattt gtcgtattca ggtcacattg aacatctggt
    33541 acatcactga tataggggtc aaatccaaaa tcagcaccac aggcacaaag acgaccgatc
    33601 gtggcggtat cgagggaaaa tcccggccat acattgctgg aggttaagcc aatatttaat
    33661 gtgacatcat atcgagataa tccctcaaga ccgacagtac cggcgaactt ttccccttca
    33721 tccaggacct ggttaatgag ttgctgaata atgccttcat cccagtatgt tgatttgcag
    33781 ttggcaatcg taatatccac gccagtgaat ggatcagtac cggagaggct tgcaaataac
    33841 gtatcaacta attccatttt tattatccgg cttaatgcaa cattttatcc atcggcacat
    33901 aacctacatt ttccaccaga ttttgtccct gtagaattaa aaatattttt ttgttttaca
    33961 ttctgttcat ccctattatt gcattttatt ttgcggtgat ggctaaaccg attctgtact
    34021 agctcaaaaa ttactgatat tccactcgca atacagccag tgcattataa cgacctgccg
    34081 caggcacttt tccagttgta ctaacaggta aagccaggaa agtaacatca gcagtttgcg
    34141 cagaagcagc ctggttttcc accaatctga acggaacgcc tactgatgtt gccagagcat
    34201 ttaaatctac ggagttttgc tgactatcca taatcataat accaacatca ggattatctt
    34261 gtgcctgaat aatactttgt ttcccattgg tatcacgtgt agctgaaatg ttaccgtcga
    34321 ataccaacgt cattaccgca tctgtatctt tattcgtaca attaatcgga atagttgttg
    34381 ctacaggagt aacaccagca ggcttcgctc cagcaccagc ccggctcaat tcactggccc
    34441 acacatccgg catattaaca gtaaaactag taccctcatt aatagtgcaa ccttgcggaa
    34501 cctgaatatc caggttactg atccaggcat taaataatgg tactggccca tgttccgttt
    34561 gccgctgact ggcatacaat gaaactaaaa gtacattatt cagagagaca tcagagatga
    34621 tttttttatc caacctaatt ttaatatagc cttctgttcc tgaagaccag taatctgctt
    34681 tacttctgtc agcaggataa ttagtactat ag
//
LOCUS       DAZGAP010000024        34505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGAP010000024 DAZGAP010000000
VERSION     DAZGAP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.024"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHLAM_001016"
     CDS             complement(<1..191)
                     /locus_tag="ACHLAM_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7852581.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSL"
     gene            complement(338..1621)
                     /gene="yeaH"
                     /locus_tag="ACHLAM_001017"
     CDS             complement(338..1621)
                     /gene="yeaH"
                     /locus_tag="ACHLAM_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO7852582.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1734..3668)
                     /gene="yeaG"
                     /locus_tag="ACHLAM_001018"
     CDS             complement(1734..3668)
                     /gene="yeaG"
                     /locus_tag="ACHLAM_001018"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO7852583.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4104..4850
                     /gene="mipA"
                     /locus_tag="ACHLAM_001019"
     CDS             4104..4850
                     /gene="mipA"
                     /locus_tag="ACHLAM_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO7852584.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYDGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4940..5794
                     /gene="yeaE"
                     /locus_tag="ACHLAM_001020"
     CDS             4940..5794
                     /gene="yeaE"
                     /locus_tag="ACHLAM_001020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO7852585.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5845..6747)
                     /gene="yeaD"
                     /locus_tag="ACHLAM_001021"
     CDS             complement(5845..6747)
                     /gene="yeaD"
                     /locus_tag="ACHLAM_001021"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO7852586.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGPAAQQGLPAHG
                     FARNLPWTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAH
                     GEFETTSALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYL
                     NPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAY
                     ASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(6831..7826)
                     /gene="gapA"
                     /locus_tag="ACHLAM_001022"
     CDS             complement(6831..7826)
                     /gene="gapA"
                     /locus_tag="ACHLAM_001022"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7852587.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8168..8581
                     /gene="msrB"
                     /locus_tag="ACHLAM_001023"
     CDS             8168..8581
                     /gene="msrB"
                     /locus_tag="ACHLAM_001023"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO7852588.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8623..8895
                     /gene="yeaC"
                     /locus_tag="ACHLAM_001024"
     CDS             8623..8895
                     /gene="yeaC"
                     /locus_tag="ACHLAM_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO7852589.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(9056..9697)
                     /gene="pncA"
                     /locus_tag="ACHLAM_001025"
     CDS             complement(9056..9697)
                     /gene="pncA"
                     /locus_tag="ACHLAM_001025"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO7852590.1"
                     /translation="MHPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(9708..10724)
                     /gene="ansA"
                     /locus_tag="ACHLAM_001026"
     CDS             complement(9708..10724)
                     /gene="ansA"
                     /locus_tag="ACHLAM_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO7852591.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(10889..12745)
                     /gene="sppA"
                     /locus_tag="ACHLAM_001027"
     CDS             complement(10889..12745)
                     /gene="sppA"
                     /locus_tag="ACHLAM_001027"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO7852592.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            12906..13457
                     /gene="ydjA"
                     /locus_tag="ACHLAM_001028"
     CDS             12906..13457
                     /gene="ydjA"
                     /locus_tag="ACHLAM_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO7852593.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAAMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGVGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            13574..14617
                     /gene="selD"
                     /locus_tag="ACHLAM_001029"
     CDS             13574..14617
                     /gene="selD"
                     /locus_tag="ACHLAM_001029"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO7852594.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRHACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRELLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            14622..16577
                     /gene="topB"
                     /locus_tag="ACHLAM_001030"
     CDS             14622..16577
                     /gene="topB"
                     /locus_tag="ACHLAM_001030"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO7852595.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(16654..17997)
                     /gene="gdhA"
                     /locus_tag="ACHLAM_001031"
     CDS             complement(16654..17997)
                     /gene="gdhA"
                     /locus_tag="ACHLAM_001031"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO7852596.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            18233..18505
                     /gene="ynjH"
                     /locus_tag="ACHLAM_001032"
     CDS             18233..18505
                     /gene="ynjH"
                     /locus_tag="ACHLAM_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HFO7852597.1"
                     /translation="MSRALFAVVLAFPIIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQQDDKNLSTNPLVWRRVKP"
     gene            complement(18471..18878)
                     /gene="nudG"
                     /locus_tag="ACHLAM_001033"
     CDS             complement(18471..18878)
                     /gene="nudG"
                     /locus_tag="ACHLAM_001033"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO7852598.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            18965..19585
                     /gene="ynjF"
                     /locus_tag="ACHLAM_001034"
     CDS             18965..19585
                     /gene="ynjF"
                     /locus_tag="ACHLAM_001034"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7852599.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(19594..20901)
                     /gene="ynjE"
                     /locus_tag="ACHLAM_001035"
     CDS             complement(19594..20901)
                     /gene="ynjE"
                     /locus_tag="ACHLAM_001035"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO7852600.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(20968..21621)
                     /gene="ynjD"
                     /locus_tag="ACHLAM_001036"
     CDS             complement(20968..21621)
                     /gene="ynjD"
                     /locus_tag="ACHLAM_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7852601.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(21621..23156)
                     /gene="ynjC"
                     /locus_tag="ACHLAM_001037"
     CDS             complement(21621..23156)
                     /gene="ynjC"
                     /locus_tag="ACHLAM_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO7852602.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQAKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLEWGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(23129..24295)
                     /gene="ynjB"
                     /locus_tag="ACHLAM_001038"
     CDS             complement(23129..24295)
                     /gene="ynjB"
                     /locus_tag="ACHLAM_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7852603.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYDINLKIVHLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQFLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(24305..24853)
                     /gene="ynjA"
                     /locus_tag="ACHLAM_001039"
     CDS             complement(24305..24853)
                     /gene="ynjA"
                     /locus_tag="ACHLAM_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO7852604.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SIFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(24853..25560)
                     /gene="ydjZ"
                     /locus_tag="ACHLAM_001040"
     CDS             complement(24853..25560)
                     /gene="ydjZ"
                     /locus_tag="ACHLAM_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7852605.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(25574..26251)
                     /gene="ydjY"
                     /locus_tag="ACHLAM_001041"
     CDS             complement(25574..26251)
                     /gene="ydjY"
                     /locus_tag="ACHLAM_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO7852606.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(26256..26966)
                     /gene="ydjX"
                     /locus_tag="ACHLAM_001042"
     CDS             complement(26256..26966)
                     /gene="ydjX"
                     /locus_tag="ACHLAM_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7852607.1"
                     /translation="MNAERKFLFACLIFALVIYAFHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(27133..27939)
                     /gene="xthA"
                     /locus_tag="ACHLAM_001043"
     CDS             complement(27133..27939)
                     /gene="xthA"
                     /locus_tag="ACHLAM_001043"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO7852608.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTANRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            28385..29605
                     /gene="astC"
                     /locus_tag="ACHLAM_001044"
     CDS             28385..29605
                     /gene="astC"
                     /locus_tag="ACHLAM_001044"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO7852609.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSGGSS"
     gene            29602..30636
                     /gene="astA"
                     /locus_tag="ACHLAM_001045"
     CDS             29602..30636
                     /gene="astA"
                     /locus_tag="ACHLAM_001045"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO7852610.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEESETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLAANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            30633..32111
                     /gene="astD"
                     /locus_tag="ACHLAM_001046"
     CDS             30633..32111
                     /gene="astD"
                     /locus_tag="ACHLAM_001046"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO7852611.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            32108..33451
                     /gene="astB"
                     /locus_tag="ACHLAM_001047"
     CDS             32108..33451
                     /gene="astB"
                     /locus_tag="ACHLAM_001047"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO7852612.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            33444..34412
                     /gene="astE"
                     /locus_tag="ACHLAM_001048"
     CDS             33444..34412
                     /gene="astE"
                     /locus_tag="ACHLAM_001048"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO7852613.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         8339 a         8740 c         9142 g         8284 t
ORIGIN      
        1 aataagctga aaataaaaaa gccttgagct tttaattttt gtgtacccat agaagccata
       61 aaaaggagag caatgtgagt gctggatttt attgatggca gagatatata gttttgacca
      121 agatcaattt tcgttaacga taaaaaataa ttaaagaata accccaggga aatacgtgtt
      181 gaccgaatca tttttccgaa tgattgtttt ctggaactaa attgtgtaaa ggttatgctg
      241 tcatatttaa agcgattgta agctaatgta tgtaataaat gagataattt acagtgaact
      301 aaaaatcagc caggcgataa tgacccggct gacagtttta gtctttagct gttgcatttt
      361 gtttatgaaa cagttcacgg aacaccggat aaatatcatc ctggtcgcgg atatgctgca
      421 tcgcaaagtt gtcgaaagta gattgcagat gctcatattc tcgccacagt gtctgatgcg
      481 cgcgacgtgt aatttcgata tagctgtaat aacgaacaac gggtaatatt tttttcgcca
      541 gaatttcatg gcaaagcgga gagtcatcgg cccagttatc gccgtctgat gcttgggctg
      601 cgtaaatatt ccactgtgcc gggttataac gctctttcac tacctcatcc atcagtttca
      661 gggcgctgga aacaatggtg ccgcctgttt cctgcgagta gaaaaactca tgttcatcga
      721 cttctttcgc ctgggtatga tggcggatgt atacgacttc cacgttctta tatgttctgc
      781 tgaggaacag atacagcaga atataaaaac gtttagccat atctttagtg gactgatcca
      841 ttgaaccgga aacgtccatc aggcaaaaca tcactgcctg gctggaggga tcgggccgtt
      901 tttcgtagtt cttgtaacgt aaatcgaagg tgtcaatgaa agggacgcgt tcaattttgg
      961 cacgtaattc tgcaatttct ttgcgcagac gttcctcttc cagcagttgc gcaggttcac
     1021 tgttgctgat gatggccaaa ttctcttcca gcgcatgaag ttcccgccgc ttgcccgccg
     1081 tcatggctgt gcgccgcgcc agtgagttct gcaatgaacg cacaacgctg atattggccg
     1141 gaacgccgtt agcggtataa cccgcccgat gcgttttata ttcggtcagc tggcgttgtt
     1201 ggttttgttt cagattcggt aaagccaaat cttcaaagag cagatcaaga tactcatctt
     1261 tcgaaatctg aaagacgaat tcatcctgac cttcaccatc ctggctggcc tggccctgac
     1321 cgctgccgga acctccgccg ccaccctggg gacgttcaat tcggtcgttc tggacgaaat
     1381 ggtcattgcc cggatgcacg cggtggcgca gaccgccacg cccctgatga aacatcggtt
     1441 cgctaatatc ttccgtggga atggatacgg actcgccgct gtcgacgtca gtcaccgaac
     1501 gcttattaat ggcctcggag atcgactgtt taatttgcgc tttataacgg cgtaaaaaac
     1561 gctggcgatt caccatgctt ttgtttttgc cgttcagacg ccggtcaata aaccaggtca
     1621 tatgcccccc gtactacatt tgccaacttt atgctacgcc tgtgttgccg cttctctggc
     1681 ccacggttcg ctgtcgttgt aggcccggta gcatgtgtac cgggccaggg ttgttaagac
     1741 gatttacgta cgcgcaaata ccattcgcac agtaaacgca cctgtttacg ggtgtagcct
     1801 ttttccatca tacggtcgac aaagtcgtca tgtttctttt gctcgtcggt tgacgttttg
     1861 gcgttaaacg aaataaccgg caacagctcc tcggtattgg agaacatttt cttctcaata
     1921 accgtgcgca gtttttcata gctggtccag ttcggattgc gtccgctgtt attcgctctg
     1981 gcgcgcagta cgaagttaac aatctcgttg cggaaatctt ttggattact gatccccgcc
     2041 ggtttctcga ttttctccag ctcggcgtta agagactcgc ggtcaaacaa ctgcccggta
     2101 tcaggatcgc gatactcctg atcctgaatc cagaaatccg cgtaggtaac ataacggtcg
     2161 aaaatgtttt gcccatattc ggaataggat tcaaggtagg ccgtctggat ctctttgccg
     2221 ataaactcgg catatttcgg gatcagataa cctttcagga actccagata gcgttctgcc
     2281 tgttcttgcg ggaactgctc gcgctcgatc tgctgttcca ggacgtagaa cagatgaacc
     2341 gggtttgccg ccacttctac atgatcgaag ttgaacacgc gggagaggat cttaaacgca
     2401 aaacgcgtcg acagaccgtt catcccttca tcgacaccgg cgtagtcacg atattcctga
     2461 tacgacttgg ctttgggatc tgtgtctttc agactttcgc catcataaac ccgcatcttt
     2521 gaataaatgc tggagttttc tggctcttta aggcgcgaaa gaatggaaaa acgtgacagc
     2581 gtttccagcg tgccaggggc gcagggggcg tgagtcaatt cactgtgatt aagcaatttc
     2641 tcgtagattt tgatctcttc ggaaatgcgc aagcaatacg gcaccttcac gatgtaaaca
     2701 cgatcgagga aggcttcgtt gtttttgtta ttacggaaag tgacccattc ggattcgttc
     2761 gagtgggcga gaataatccc gttgaacggc agggcggaga taccttccgt cccgttgtag
     2821 ttaccttcct gagtggcggt tagcaaggga tgcagcactt taatcggtgc tttaaacatc
     2881 tcaacgaatt ccatgatccc ctggttggcg cggcacagtg caccggaata gccgtaggcg
     2941 tccgggtcat tctgcgcgta gtgttcgagt ttacgaatat cgactttccc aaccagcgcg
     3001 gagatgtcct ggttgttctc atcaccgggt tccgttttgg cgatagcaat ttgttgcaga
     3061 attgacggcc agaccttcac tacccggaac ttagtgatat cgccaccaaa ttcatgcagg
     3121 cgttttgccg cccacggcga catgatggtg ccgagataac ggcgaggaat gccatactct
     3181 ttttccagaa tctgcgcatc ttcctgcgga ttgaaaagac agaacggatg atcgttgacc
     3241 gggctacgct caccgttggc gctcaataca taaatcggta cgagctgcat taatgatttc
     3301 agtcgctcag caagcgatga tttaccccca cccacaggcc caagcagata caggatttgt
     3361 ttcttctctt ccagcccctg agccgcgtgt ttcagataag agacaatctg ttcaatcgcg
     3421 tcttccatgc cgtaaaactc ttcaaatgcc ggataacgtg ctatgacccg gttagaaaag
     3481 agtcgagaaa gtctgggttc ctgggctgta tcgaccatga caggctcacc gatagccatc
     3541 aatagccgct cagccgcgtt ggcataagca ctgcgatctt gccgacaagt ggtaagaaac
     3601 tcctgcagtg tgaactcttc gtccttggca gcttcatatc gctggcgata gtgatcgaat
     3661 atattcatgg tatgccgtcc tttcgttttt tagcacaggt taagagccgt tcatatgaat
     3721 agtagaggct cccggaagaa gtaaactgaa cgacgtctct tgctaaaaca cagcaactct
     3781 catgccattt tatcgacgat gagaacaacg tgcccggtga tacaccttca aaattaagcg
     3841 tagatggcaa atggaaaact tgcctgcacg gctttcttaa tttcaatggc atatcaacaa
     3901 ctcatccata aattttgcat aattaatgta aataccaggg tcgccggtaa cgctaaattc
     3961 atttggctgt aagagtggtg tcatctgcgt caggaaaatt aaacagttac tataaaaaat
     4021 gaaaacgtaa aaaggttggg tttcgatgta ttgacgggta aactttgtcg cccgctaaac
     4081 atttgtttaa ggaatgatta attgtgacca aactcaaact tctggcactt ggagtgctta
     4141 tcgcaacgtc tgcaggcgta gcgcacgctg aaggtaaatt ttccctgggc gcaggcgtag
     4201 gtgtcgttga acacccatat aaagattacg ataccgatgt ttacccagta ccggtaatca
     4261 actatgacgg cgataacttc tggttccgtg gcttaggtgg tggttactac ctgtggaatg
     4321 acgcaacgga caaactttca attaccgctt actggtcgcc gctttacttc aaagctaaag
     4381 acagtggcga tcaccaaatg cgtcacctgg atgaccgtaa gagcaccatg atggctggtc
     4441 tgtcttatgc tcactttacc cagtacggtt acctgcgtac caccctggct ggcgataccc
     4501 tggataacag caacggcatc gtctgggata tggcctggtt gtatcgttac accaacggtg
     4561 gcctgaccgt gactccgggt attggtgtgc agtggaacag cgaaaaccag aacgaatact
     4621 attatggcgt atcgcgcaaa gagtccgctc gcagcggtct gcgtggctat aacccgaacg
     4681 acagctggag cccttacctg gagctgagcg ccagctacaa cttcctcggc gactggagtg
     4741 tttacggtac cgcgcgctac acccgtctgt ctgatgaagt tactgacagc ccaatggtgg
     4801 ataaatcctg gactggcctg atttctaccg ggatcaccta caaattctga taatgcatct
     4861 ttccagggca ttttgtgcat aaaaacaggg cgtcatgccc tgttttgcat tacggtggtg
     4921 caatacctgg gaggaaacaa tgcaacaaaa aatgattcaa tttagtggcg atgtctcact
     4981 gccagccata gggcagggaa catggtatat gggcgaagat gccagtcagc gcaaaacaga
     5041 agttgctgca ctacgcgcgg gcattgaact cggtttaacc ctcattgata ccgccgaaat
     5101 gtatgccgaa ggcggtgctg aaaaggtggt tggggaagca ttaaccggtc tgcgtgataa
     5161 cgtctttctc gtctctaaag tctatccgtg gaatgctggc gggcaaaaag cgataaatgc
     5221 atgcgaagcc agtttacgcc gtctcaatac tgattatctc gatctctact tattacactg
     5281 gtctggcagt ttcgcttttg aagagactgt cgcagcgatg gaaaaattga tcgcccaggg
     5341 aaaaatccgc cgctggggcg tttctaacct tgattatgcc gacatgcagg aactctggca
     5401 gctgccgggg ggaaatcagt gtgccactaa tcaggtgctt taccatctcg gttcacgagg
     5461 gattgagtac gatctactcc cctggtgcca gcaacagcag ataccggtga tggcttacag
     5521 tccgttagct caggccgggc ggttgcgcaa tgggctgcta aaaaacgcag tagtcaacga
     5581 aattgcacat gctcacaata tcagcgcggc acaagtattg ttggcgtggg tgatcagtca
     5641 tcagggtgtg atggcgattc caaaagcggc cacgattgcc catgtccaac aaaatgcggc
     5701 tgtgcttgag gttgaacttt cgtcagcgga attagctatg ctggataagg catatccggc
     5761 accaaaagga aaaactgcgc tggatatggt gtgatgttga tacgtgccgg atgcaacgca
     5821 cccggcacac aacattaacg caaattaacg tttcgcaacg cgaatggatt gcgccagatg
     5881 tgcaggtttt tctttggtca ctttttgcgt ttctgaagcg taagccgttt ctacacaaac
     5941 aaaggttttg tagccatcgt ccggcatatc gcccatgcta actgaaagcg ccggtcctgg
     6001 gttccagcca acgacgttca gatgatgctg gtggcctacg gcgataatac gattcagcgc
     6061 ttcgtcatta atcacgctgc aatcttgcgg attcagatac acgcgatcgg tacggtcagg
     6121 gaaggtctga ataccgtcgg tcagtacatc ttctttcgcg tcattcactt tatcaatgaa
     6181 gcgatcgccc agtccactga cgcttacctt agtgatatca cccacgttaa agtaggtatg
     6241 cagggcagag gtggtttcaa attcgccgtg tgcttcaaga tcgatttcac aggctttacc
     6301 cacgcggaaa tgcgctaata gcgtaaagtc gtgcggccag aattttttcg tctcttcgct
     6361 ttgtgtcaat tcaaaagtca gcgctacgcc atcagcatct tcacgatgtg atttcagcgt
     6421 ccacggcagg ttgcgggcaa aaccgtgcgc aggcagacct tgttgtgcag ccggaccttg
     6481 ttgtgcagcc ggaccaaacc acggccagca aaccggtacg ccaccgcgaa tagcgacgcc
     6541 atttttgaac ggtgtgttat tgctcaacca cagaacttct tcttcacccg caggtttcca
     6601 tgagagaagg tgtgcgccct gtaatgcaaa agaggctttt acctggggat gatcgaccac
     6661 aatgaggtcc agttcatcca gtttacgacg ggagaggaca ggggagattt gttcgatgac
     6721 cggaagggca aaaattttct taatcatgac gcagtccttt aacttcattt tatcaggtaa
     6781 aaaaaagagc gaccgaagtc gctcttttta gatcacagtg tcatctcaac ttatttggag
     6841 atgtgagcga tcaggtccag aactttgttg gagtaaccgg tttcgttgtc gtaccaggat
     6901 accagtttca cgaagttgtc gttcagagcg ataccagctt tagcatcgaa cacggaagtg
     6961 caaacttcgc cgttgaaatc ggtagatact acgtcatctt cggtgtagcc cagaacgcct
     7021 ttcatttcgc cttcagcagc agctttaacg gcagctttga tctgctcgta agttgcagct
     7081 ttttccagac gaacggtcag gtcaactacg gatacgttcg gggtcggaac gcggaacgcc
     7141 ataccagtca gtttgccatt cagttctggc agtactttac ctacagcttt agcagcaccg
     7201 gtagaggacg ggatgatgtt ctgggaagca ccgcggccgc cgcgccagtc tttgtgagac
     7261 gggccatcaa cggttttctg agtagcggta gtagcgtgaa cggtggtcat cagaccttcg
     7321 atgatgccga agttatcgtt gataacttta gccagcggag ccaggcagtt ggtggtgcag
     7381 gaagcgttgg aaacgatgtc ctggccagca tatttgtcga agttagcgcc tttaacgaac
     7441 atcggagtgt tgtctttaga cggaccagtc ataaccactt tcttcgcgcc agcggtgatg
     7501 tgtttacgag cagtttcgtc agtcaggaac agaccagttg cttcagcgac aacgtcaaca
     7561 ccaacttcgt cccatttcag gttagccgga tcacgttcag cggtaacacg gattttttta
     7621 ccgttaacga tcagatgacc gtctttcact tcaacggtac cgtcgaaacg gccgtgagtg
     7681 gagtcatatt tcagcatgta tgccatgtaa tcagcgtcta acaggtcgtt gattgcaacg
     7741 atctcgatgt cagaacgttt ctgagcagca cggaaaacaa tgcgaccgat acggccaaaa
     7801 ccgttgatac ctactttgat agtcatatat tccaccagct atttgttagt gaataaaagg
     7861 ttgcctgtaa aattacaaaa accttacgca gcgtcaagcg gaatcgtgtc aatcattgcg
     7921 acaaatcaat cctgtgccta agcattacgc gactgactcg cctcactctt cctttgggct
     7981 tgagaccaca tggggtcggc gccccgaatt ttaaagggca attacgataa aaatgtgatt
     8041 ttcatcacga tttaggtgca gccatttcgt cgagatcaag tttaggacaa aattttgcca
     8101 gttggtgtta atgttttgtt agaatcagtc acgtaatgtg agcacgatta aagtgagatg
     8161 tgagcaaatg gctaataaac cttcggcaga agaactgaaa aaaaatttgt ccgagatgca
     8221 gttctacgtg acgcagaatc atgggacaga accgccattt acgggtcgtt tactgcataa
     8281 caagcgtgac ggcgtatatc actgtttgat ctgcgatgcc ccgctgtttc attcccaaac
     8341 caagtatgat tccggctgtg gctggcccag tttctacgaa ccggtgagtg aagaatctat
     8401 tcgttatatc aaagacttgt cacatggaat gcagcgcata gaaattcgtt gcggtaactg
     8461 cgatgcccat ctgggccatg tcttccctga cgggccgcag ccaacgggcg aacgttattg
     8521 tgttaactct gcttctttac gcttcactga tggcgaaaac ggcgaagaaa tcaacggttg
     8581 aaaaaacgat tcagcaactt attccacagg agcggaaaaa ttatgaatct tgatgacatt
     8641 atcaacagca tgacgcctga ggtataccag cgtttgtcga ccgccgttga actggggaaa
     8701 tggcctgatg gcgttacgtt gaccgaggaa caaaaagaga actgcctgca actggtgatg
     8761 ctgtggcaag cccgcaataa taccgaagca cagcatatga caattgacac aaatggtcag
     8821 atggtcatga agagcaaaca acagttaaaa gaagattttg gtatctcagc aaagcctatt
     8881 gccatgttta agtgaatgag ttgtctagta tttaagcctg ttattaaggg agggcaaata
     8941 gacttgttcg gatgtcgtgt tttagctgcg agtaggttaa atgcaacact aacaagtatt
     9001 acctggccta caatgtcatt gaggaaatgt aggccagaga tgaatccaac taatattact
     9061 cctgcgtctc ttcccaatct gccagcgtat atagcgttgc cccagctgct gacatctcca
     9121 taaacgcgtg cgcactgtcc tgtggctgga tattcacgcc acgacaacca tcggtaatca
     9181 cattaacctt ataacctaac tgtaacgcgt ccagcacggt aaacttcacg caatagtcag
     9241 tagccagacc cataatgatc aattcatcga tttcatgatc gcgtaaccag tcatcgagcg
     9301 tggttttctg ccgacggccg ttatcaaaaa aggcactgta actgtcaact aaaggatttt
     9361 cgcctttatg gaacaccgct gcgatcgctt tttggttcag taacggatgt aattgtgcgc
     9421 cttcactgtt ctgcacacag tgatctggcc agaaggtttg tggcaaaccg tcgagttggc
     9481 ctggcgtata aggctctaca ccgtgctgac tggcaaaact gccgtgattc gccgggtgcc
     9541 agtcctgact ggcgataacc gcttcaccgc gcgactggca ccagtcaatc aggcggttag
     9601 cgacatccac cgtactgtca ccttccggca cggcgagcgc gccaccagca cagaaatcat
     9661 tttgtaaatc gaccagtaac agggcgcgag ggtgcattgc agtctcctta atcatccggc
     9721 gtcagttcgc cacgcaggtt ttggctcatg gccttgcgaa tggtttcagt atcaagttcc
     9781 tggctaagca ggtaatgcag tttggttagc gtggcttcta cagtcatatc tgcaccgcca
     9841 ataacgccgg catgggcgag ggcgttaccg gtggcataac cacccatgtt cactttaccg
     9901 gacatacatt gtgtcaggtt gaccaccaca ataccgcgat cgctggcttc ttgtaattcc
     9961 tgcaggaagg ctttgttttg tggcgcatta cccacgccat atgagcgcag aatcaatgct
    10021 ttcacaggtt ggcgcagaaa attgcgcacg acgtcagcag aaatccccgg ataaatcgtc
    10081 actacgccaa ttggttgcgg cgtgattgga tgaacgatca attccccttc accgtgcggg
    10141 gcgggtggcg tattcaaacg gcgaatatgg atacctgctt ccagtaacgg aggaaggttt
    10201 ggagaggcaa acgcatcaaa accatcggca tgggctttgg tggttctgtt gccacgatac
    10261 agtcgattat taaaaaagag agttacttcg ttgataggat aattcgccgc aacgtacaac
    10321 gcattcagca gattaatttg tccgtcagag cgtaactcag ccagcgggat ttgtgaccct
    10381 gtcacaatga ccggtttacc gagattctcg agcatgaacg acagtgcaga ggcggtatac
    10441 gccatcgtgt ctgtgccgtg cagaatgaca aaaccatcat agtcgtcata gtgcgcttta
    10501 atatcttcag caatatgctg ccagtcttct ggcgtcatat cagaagaatc catcaatggc
    10561 gtatattcat gaatagtgaa atctggcatc tccgggcgat ggaattccgg catcagcgcc
    10621 agttggcgct gtagatgacc tgacaccgga atataaccct gctcggaacg ctgcatcccg
    10681 atggtcccgc ccgtgtaggc gacgtaaatt gatttctttt gcatgatatt gatatgaatg
    10741 atatcgaaag agcaaaagta tatacgtgag gaagggggag cgcacctgat actatgcgga
    10801 taaggaaata tggggcgaga cagcgggaag aggattcgtc gaggtaaaaa aatgtggccg
    10861 acgatctgtc ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg
    10921 cataacggtt ttgtgggtcg ttaaacgctg ccagcttgtc gctttcactt ttaacggtag
    10981 aggctaccga ggccagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc
    11041 ggacagaacc agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt
    11101 attccagatg ccactgtttc acttttgcca gctctgctgc tttggcgacc gcatcatcga
    11161 aatccccgag actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacatggc
    11221 cctgggcgat tttatcaatc tgctctggcg ttgaatgacg cgcatcagca accagcgtga
    11281 taaagcgttt atagccattc tcaatgctta actgcatcat ctgctgcgct tccggcggca
    11341 gtgccctggt gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac
    11401 caatcgaatc cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg
    11461 tcagggtgct ggggttagcc acaatgtaat tagctggtgt ggaaatccag taaccaccag
    11521 atgccgccat gccgcccatc gatacaacca caggcttacc cgctgcccgg gctgctgcca
    11581 gttcagcgcg aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga
    11641 caatcgcttt cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac
    11701 cgccaacatt cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgactc
    11761 cgatgctgtc accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc
    11821 gataattttt atcagtcttg ctccagccga actctttggt cagagttttt tcgatttcgg
    11881 cactcgatgc cagtgcatcg accagcttgt tttccagcgc atatttcgcg gtatcgccac
    11941 cggttttggt taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga
    12001 tctgccggtt agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc
    12061 tgtcagcttc gcgggctgcc ggcgacatat catcacgaat aaacggttca acggcagatt
    12121 tatacgtacc cacgcggaac acatgggtgg aaactttcag cttatccagt aatgatttgt
    12181 agtacagacc gttggtggca aagccgtgca gatcaaccac gccttgcgga gacagccaaa
    12241 ttttattggc gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat
    12301 aaaccggttt cccgctatca cgaaactctt tcagagcttt gccgatgtac tgcatagacg
    12361 gctggtcgcc gcctgcgaag tttttcagat ccatcacaat accggtgata ttgcggtcgt
    12421 ccttcgcctg gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg
    12481 aactggcacc aagcagctgg cggcttaatt tactaaaccg ctgagaactg tcgggtttat
    12541 cgacgatcac accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat
    12601 caccaccact gacctgcatc cagatcccca caccaaccag cacgagaaaa ataaagaaca
    12661 ggttaagtac catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg
    12721 caataaatcg ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtttcagg
    12781 cgcaatagat gtggttatcg taatgaccca accggcactt gtcagcagga attgcggact
    12841 gggctgtaac aaaatccact ctcgtgttaa ttttgtgaat aaatatcatg acaggagtta
    12901 atcaaatgga tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac
    12961 ctgcgccaac gggtgaacaa ctgcaaaaca tcctgcgtgc ggctatgcgt gcgccggacc
    13021 ataagtccat gcaaccgtgg catttctttg tgattgaagg ggaagggcgc gagcgtttca
    13081 gcgccgtact ggagcagggg gcgattgctg ccggtagtga taacaaagcc atcgacaaag
    13141 cgcgtaatgc gccgttccgc gcaccgctca tcataacggt ggtggcgaaa tgcgaagaaa
    13201 atcataaagt cccgcgctgg gaacaggaaa tgtctgcggg atgcgcggtc atggcgatgc
    13261 aaatggcagc agttgcccag ggggttggcg gcatctggcg cagtggtgca ttaactgaaa
    13321 gtccggtagt gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct
    13381 acctcggtac gccgcagttg aaagcatcta cgtcgataaa cgtcccggac ccgacgccgt
    13441 ttgtgactta tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga
    13501 attcagacac tctcacttat cacttcatgg aatgagggtt accatacccg gattgcattg
    13561 acaggagatg tccatgagcg agaactcgat tcgtttgacc caatacagcc acggcgctgg
    13621 ttgcggctgt aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa
    13681 gtttgttgat ccgaatttgc ttgtgggtaa tgaaacccgc gacgatgcgg cggtatacga
    13741 tctgggcaat ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa
    13801 tcctttcgat tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcaatggg
    13861 cggcaaaccg attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga
    13921 aattgcccgc gaagtgaccg aaggtggacg ccatgcatgc cgtcaggcgg ggattgcgct
    13981 ggctggcggt cactccatcg atgcgccaga gccgattttt ggtctggcgg taacggggat
    14041 cgtacctacc gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct
    14101 gacgaaacct ctggggattg gcgttcttac cacggctgag aaaaaatcac tgctgaaacc
    14161 agaacatcag ggactggcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt
    14221 tgccaacatc gaaggcgtaa aagcgatgac cgacgttacg ggttttggtt tactggggca
    14281 tttaagcgaa atgtgtcagg gcgcaggtgt gcaggcgcgc gtcgactatg acgcgatccc
    14341 gaaattgccg ggggttgaag agtacattaa gttgggcgca gtgcctggcg gcaccgaacg
    14401 taactttgcc agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgaactgct
    14461 gtgcgatccg caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga
    14521 ggtcaaagct acagccgccg agtttggcat tgaactgacg gcgattggcg aactggtgcc
    14581 agcgcgcggc ggtcgtgcca tggttgagat ccgttaattc aatgcggttg tttattgccg
    14641 aaaaaccgag tctggcgcgc gccattgctg atgtcctgcc taaaccgcac cggaaaggcg
    14701 atggctttat cgagtgcggt aatggtcagg tggtgacctg gtgtatcggt cacctgcttg
    14761 agcaggcgca gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc
    14821 cgattgtccc ggaaaagtgg caattacagc cccgaccctc cgtgactaaa caacttaatg
    14881 tcatcaaacg cttcctgcat gaagccagcg aaatcgttca cgccggagac ccggatcgcg
    14941 aagggcaact gctggtggat gaagtgctgg actacctgca actggcaccg gaaaagcgcc
    15001 agcaggtaca gcgttgtttg ataaacgacc tgaacccgca ggcggttgag cgggcgatcg
    15061 atcgtcttcg ctccaatagc gagtttgtac cgctgtgcgt ttctgcactg gcgcgtgcgc
    15121 gagctgactg gctgtacggc attaatatga cccgtgcgta taccattctc ggtcgcaatg
    15181 ccggttatca gggcgtgctt tccgtgggac gcgtgcagac gcccgtactc ggactggtgg
    15241 tgcgccgcga cgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac
    15301 atatcgtgac acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg
    15361 aaccgtacca ggatgaagaa gggcgcttat tacatcgtcc actggcggag catgtggtta
    15421 accgcattag tggtcaacca gctattgtca ccagctataa cgataaacgg gaatcagaat
    15481 ccgcgccgct gcctttttcg ctttcggcgt tgcagattga agcggctaaa cgttttggtc
    15541 tgagcgcgca gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca
    15601 cttatccgcg ttccgattgc cgctatttgc cagaagaaca ttttgccgga cgccacgcgg
    15661 tgatgaatgc tatcagcgtt catgcaccag atctgttgcc gcagccagtg gtcgatccag
    15721 atatacgcaa ccgctgttgg gatgacaaaa aagtcgatgc gcaccacgcc atcatcccga
    15781 ccgcacggag ttctgcgatc aacctgacgg agaacgaagc gaaggtctat aacctgattg
    15841 cccgtcagta tttgatgcag ttctgcccgg atgcggtgtt ccgcaagtgt gttatcgaac
    15901 tggacattgc caaaggcaaa tttgtcgcta aagcccgttt tcttgctgaa gcaggctggc
    15961 gcacactgtt aggcagcaaa gagcgcgatg aagaaaacga cggcacgccg ctgcctgtgg
    16021 tggcgaaagg cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc
    16081 cgccgcgcca ttttaccgat gcaacactgc tttcagcgat gaccgggata gcacgttttg
    16141 tgcaggacaa agatctgaaa aagatccttc gtgcgaccga tggtctgggg acagaagcga
    16201 ctcgcgccgg gattattgaa ctgctgttca agcgaggctt cctgaccaaa aaagggcgct
    16261 atatccactc caccgacgcc ggaaaagcgc tattccattc gctgccagaa atggcgacgc
    16321 gaccggacat gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc
    16381 gctatcagga ctttatgcag ccgctggtgg ggacattgta ccagttgatt gatcaggcaa
    16441 agcgcacgtc ggtaaggcag tttcggggaa taatggctcc cggcggtgga gaagggaaga
    16501 aaaaggattc gccacgcaag agagcgccga aaaaaagccc gccatcagaa gaggcgggca
    16561 atggagtgat aatataaagg ttctttcaac catctgatac tgaagatttt tatggccgga
    16621 taaggcgttt gcgctgtatc cggcacaatt gtcttaaatc acgccctggc ttaacatcgc
    16681 atccgcaacc ttcacaaaac cggcaatgtt cgccccctgc acgtagttgg tttgctcacc
    16741 ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat gcaaacgtgc
    16801 gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgcgccattt ccaggcccga
    16861 tgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc ctgcctgctg
    16921 gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcggcga cggctttaac
    16981 gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg tggcgcaagg
    17041 cagggcgata tcaaccggta cagaccacgg ttgttggcct tcgagataga ccagaccaaa
    17101 ttctttggcg taatcagcca ctcgaccatc gcggctggct ttgatttcga taagacgtgc
    17161 cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt ctgacgcagt
    17221 gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga cgttgccgga
    17281 gccagaaacg gaaactcgca tcccttcaaa acccataccg tggcgtttta gcattgcttc
    17341 ggtgaaataa accagaccgt agccggtagc ttccgggcga ataagactgc cgccaaatga
    17401 aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca tcatccccgc
    17461 cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt cagtatccgc
    17521 gcccaggtgg cgatacagtt cagtcatcag cgcctggcaa aaacgcataa cttcgccttc
    17581 gcttttccct ttcgggtcga aatcgctgcc gcctttacca ccgcccatcg gtagagtagt
    17641 cagggcattt ttgaaggttt gttcgaagcc gaggaatttg agaatggaga ggttaactga
    17701 cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact gcacacgcca
    17761 tgcacggttg acctgcacct ggttgcgatc atcaacccat actacgcgaa attggatcac
    17821 gcgctccggt tcaaccagac gctccagtaa tgacatctga cgatatttgg gattttgttc
    17881 aagaaaaggc cagagtgtgg tcattacttc acgaacggct tgcgcgaact cggtttgatt
    17941 cgggtcgcgc ttttggacat ggttgaggaa tgactccaga gaatatgtct gatccataga
    18001 tataaaaccc ttatatatta atacgattat gcttatgttg tttatgtcat gtgcttttgc
    18061 agttttcgac tataccatca agaatgtgac ggtaagcaag aaaaaaatga cccaggaaag
    18121 caaattaata acgagagtaa tctcataacc aaatgcgata actcagacgc taaagttttg
    18181 ccacccggct accgttatca gtagcagaga caataacgtt aaggagttcc ctgtgagtcg
    18241 agcattgttc gccgtagtat tagcatttcc aataattgca ctggctaacc cgcattatcg
    18301 tccggacgtg gaggtaaacg taccgccaga agtgttcagt tcaggcgggc agtcagcgca
    18361 gccctgtact cagtgctgcg tttatcagga tcaaaattat tctgaagggg cggtaattaa
    18421 agccgaagga atactgctgc aatgccaaca ggatgacaaa aaccttagca ctaatccgct
    18481 ggtctggcgg cgcgtaaagc cataaacgcc tctaataatg gaatgtcagc aggggccagc
    18541 ggatattgca gcgcctcttc aggtgagcac cagaccagcg cctgatgttc atgtgcctgt
    18601 aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat ggataatccg ccccgaaact
    18661 tctcgctgat ggctggcaac atagtcacca acagttgctt cgatgcccag ttcttcgttt
    18721 aactcacgca ccagcgcctg ccgctggctt tcatccggct cgactttacc accggcaaac
    18781 tcccataatc ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat tttgccatca
    18841 cgttcaatga tggcggcaac aacttcaatc attttcataa attacacacg ggcagataag
    18901 cgaaaaatgc cataatatcc gcactgttgc tcaatcggaa aggctttata tttaaggagt
    18961 gcctgtgcta gaccgccatc ttcatccccg aattaaacct ttgttgcatc agtgtgtgaa
    19021 ggtgctggat aaacccggca tcacgccaga tggtttaaca ttagtgggat ttgccatcgg
    19081 cgtgctggca ctgccgtttc tggcgctggg ctggtatctg gcggcgctaa tcgtcatttt
    19141 gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag gacttaccga
    19201 cgcgggcggc tttctcgata tttctctcga ttttctcttt tacgcgctgg tgccgtttgg
    19261 ttttattctc gccgcaccag aacaaaacgc gctggccgga ggctggctgt tgtttgcgtt
    19321 tatcggcacg ggcagcagtt ttctcgcttt tgctgcgctg gcggcgaaac atcagattga
    19381 taatcccggt tatgcgcata aatcgtttta ttatctgggc ggcttaaccg aaggcagcga
    19441 gacgattttg ctgtttgttc tgagttgcct ctttccggcc tgttttccgt ggtttgcgtg
    19501 ggtatttggt gcgttgtgct ggatgacgac ctttacgcgg gtgtggagtg ggtatctgac
    19561 gctgaagtcg ctccagcgtc agtaattgca atgttattta ctgctgtccg ggccgcgttc
    19621 accggttgct accggatttt ttggatcgct gctccattcg taccagccac cgtcataaac
    19681 ggagacgtta ttccagccca ttgcgcgtgc gtacataaag gtttctgacg cgcgccagcc
    19741 ggtgccgcag tagaatgaaa cttgctgatc tggcttgata ttccatgctt tccacatagc
    19801 ggtaatatca tcggcgctac gcatggtgcc atccgggtta tggaaatctt ccatatgcgt
    19861 cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt tcaccttttg gtttaatata
    19921 gctgtaaccg ctggtcgtac cgataaattc tggccacgaa cgaatgctca ccagcgatgc
    19981 atcctggcga tgcagcagtc cacgcgcttg ttcaatatca agcatcagtt gcggttgtgc
    20041 cgggatcttc acgccgaaat ccggttccgc tttcactttc ggtggcgttc cgcgctcaac
    20101 gggcagtccc gcgtcggacc aggtttgcca gccgccgtcc agcaggcgga catctttcac
    20161 gccagcgtag agcataattt gtgccacacg cgctgcagcg tatacgtcac gcccgtacag
    20221 aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc atcgctttta gttgttcatc
    20281 agaaactttg ttccacagcg gttcgctttc cacttcgttg gtatcgatgt agtcagcgcc
    20341 gggaatatgg ctgataaggt aaagcttagg agcgccccag gccgcttcaa tgactttcca
    20401 gtcaccggca ggtttcgccg taacctcttt accttgttgc aggtcgtgca gccattgcgg
    20461 ataaaccagc tgctcaaaat gcggcagttt ttgcagacgg gaaggttcgc ttagcgcgtc
    20521 actcagaatg gagatatgcg taaaacctgc tttttgcagt cgctttttga cggcgtcgac
    20581 atctttgtca ttaccgtaca gcgccaccgg agcatcggct ttcaggttat gttgcttgat
    20641 ccactcgttg agctgttcgg tgctcatttt gtcgagccag ttagcagaga ggtttaaggc
    20701 ggcaggttca tgaccagaag ggccatttaa ggtttgtggc cagccgttat aaaacgcgct
    20761 ggggcgagta tctatcgctt tgccattttg ttgttgaagc tggtcaagtg taagaggctt
    20821 cgccagttca gcggcccacg aagaagcgca agccagccct aaagccattg ccagcgcggt
    20881 catttgagaa acacgtttca tcgaaaatcc cgacattgta gttttgagtg cgccattctg
    20941 aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg ttgtaatttt ctgaccactg
    21001 cgccatatcc agaacagaac tatcagcagg aacatcctgg agatcgtgcg ttacctgaac
    21061 gacggggatc gcctgggcgc gaacttcgct gaacacccac tggcgaaaat tatcgcgcag
    21121 agccacatct agacggctga atggctcatc caggagtaac gcttttggct gggcgagaag
    21181 ggcgcgtagc agagcaacgc gcgctcgctg accgccagac aaagtagcag gatcctgatg
    21241 gaaagctccg tcaaggcctg aacgctcaag tgcatcattc acggcattac gtcgggcatt
    21301 ccctttaagt gtcgccggta gcgccagcag taaattttgc ccgacgctga actggtcaaa
    21361 taacagtgca tcctggaaaa gaatgccaat ctgacgctgt gcggtgggta gcatgtcaat
    21421 ccgttgctca ttgagccata gctcccctgt acaagaaaac tgtccggcca gtccaccaat
    21481 catccatgaa aacagagtgg attttccaca gccagacggc cccattaacg tgacaatgtc
    21541 acctttatcc accgtaaagt taacgtttgt cagcaagcgg ctttctggta aacgtagcga
    21601 aacatttttc acgcagagca ttagcggagt ccttgtctga cataacctac ccattttgcg
    21661 actaacgcgg tcagggcaaa aataataagc ggtaatagca gttgccataa agcctgggcg
    21721 gcgagaatgc cgttgctgcc gccgctgctt aatgccaccg cttcagtggt gagcgtcgga
    21781 aaacgccccg cgcccagcca cagcgtcggc atatactgtg caatactgac tgaaaatccc
    21841 accgcgaagg caatcagcgt agggcgtagc ataagcggac atttcacgta aaagaagatt
    21901 ttggaccgcg accagcccag tgtttgcgca atcaaaatta accgtgaatc aatgcgctgc
    21961 caggcgggtt gcaggataaa cagcatccac ggcattaccc acagcagata cccccagacc
    22021 accgccgtcc agcttccatc taggtttagc catagcgcca gcgtgtactg gcccgccacc
    22081 agtggcagag cgggtaataa aatgggcaac catagccaga aatgccgacg tgacgatccc
    22141 cattccagcc acgccagtat caggattaaa gcgataaacg tcgcggtaag tcccatcgtc
    22201 aggctattga tgagcgcttc actattgatc gtcgactgat ccgcgaggat cgccaacaga
    22261 accacacaca gcacaccggt caagggtaaa aaactcgcca gagtaatgcc aggcaataaa
    22321 ggcgtggcag gcttacgaac gccatctacg cggggaatag tacgcctcca gctacgccat
    22381 agcagatagc ccagcaaaac gtaggcggcg agtaacagca tcagcagcag gctggcgagt
    22441 gcgcctttcg cttgttgatc ggcgtcgccc tgagttaacc actgccagct aattactgcc
    22501 agcgtcgggg gattacctgg cccaaggata atcgccacat ccacgaccga cagcgaccag
    22561 gccacaatcg ccagcatcgc cattgccagc gcaggcgcga cagagggcaa cagcagccag
    22621 ttcaggcatt gccagcggct gtagcccagc gaatccagca caatgagttg ctgcaacagc
    22681 cgtttttcgc tcaatactgc cgctaagatc cacagcagaa atgcgctttc tttcactgcc
    22741 agggtgaggc ccagcccaat acccaatttg tccattggcg gagtgaaata cgggaaatag
    22801 tcataaagca gccctccgtc agcaaagagc agaagggcgc tggtggcaaa agccacatgg
    22861 ggaatggcga gcagccacgg cagacgggcg cacatacgct gccatttcgg ccctggccac
    22921 agcgctacaa tcaccagcag ggcaatcaac aacgccccga ccgccgcaat ggttgtcgac
    22981 accagcgttg ccagtaatgc ctgcggtaac tgtggatcgg caaataacgc ctgccagtgc
    23041 gtcaacgaca aagcaggcga tatcagcgtg agaactgccg ggatcagcgg tgcataaatc
    23101 accgccatca tcgcccacag caaaaagatt aatgcgtacc gtaacggtgt agccattctt
    23161 gttccagcgc atttacccaa cctgcgtgcg gttcagccag taccggcggc agatcctgcg
    23221 gcattctcga ttgcaatgat tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag
    23281 aaggatcgcc ccagacagcg ggatctgctt tacgcagttg tgcatcgggt gagagcagga
    23341 aattggcaac taccttcgcc gcagcactgg cattcgcgtt ggcgggaatg gtgacgaaat
    23401 ggacgttgcc aatcatcccc tcgctaaagc caaagctgta actgctggcg ggtaaatcgc
    23461 cgctggcgat tttttgctgc gcatgagcag gattaaaggt cagcgacagg cgcaatgtgt
    23521 tagctttcag cagagcatcc atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc
    23581 ataaatacgg atgcagcgca tcaagaaatt gccacaaggg cgcagtgaca cgggcgaaag
    23641 tcgcatcgtc cggcgcttct tttaatgcgg cgggatcggg tgtcaacata atcagcaatt
    23701 gttcaagaaa cgccgtgccg gtaaagtccg gtgggcgcgg ataggtaacc gtgccgggat
    23761 tggctttagc aaactccagt aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc
    23821 ggcgggcgat aaacgtcagt tgtgcgccgc cccagggcga ttcagcccct tctgtaggca
    23881 ctgaaaaatc ttcccgcacc ggaagctgtg tgtcgacata gcgccagttg ggcagagtct
    23941 ctgcccagtc ggtttgcagt aaattagcct cttttaaggt gcggaagttt tcgccgttca
    24001 cccagagcag atccaccgaa ccgcctgttt tgcgtccggc agcagcttcg gtctgaatgc
    24061 gcttcaccgc gtctgcggca tccgcgagat gaacaatctt caggtttata tcgtaatggg
    24121 ttttcatctc gccgctaacc cagtcgagat agcggttaat tgcggtatcg ccgccccagg
    24181 cgttaaacca gacggtttgc cctttggcgt cgtttttgat ttcctgccag tcactggcat
    24241 gtgttgccaa agaaaacagc gataacaatc ccagcaacca cacacaatgg cgcatcatct
    24301 ctccttaaga acgtttttca ggaatacggc acagcccctg agcgggaatg tccatggcag
    24361 aattaaaacg ggctgacagg ttttgcagtc cgatcaatgc cgtcagttcg gtgagcgcct
    24421 gagcgtcaaa atgcgtaacc agtcgggcgc gcagggcatc atcgaccgtt ggcggcgtta
    24481 cgcttgcggc ttctgcgtac tccagcgcca gccgttcttc atcgctaaag agcgggcttt
    24541 gccgccagtc agccactgcc agcagtttat cggtgctgcc agtgcgctcg gcgactttca
    24601 tactggtgat atccacgcaa aactcacaca ggcacatttg cgcaatgcgc gcgctgacaa
    24661 gcgatcgcac taccggatcc agcggtgagc gtttgcgctc cagccagccg acaaatatcg
    24721 acaccagata aaagataaac gggatccgcc cccaccagcg aatcgggctt agcacctcgc
    24781 cgtaatggcg acgatgcagc cacgcctgcg gacgtaaaat gaaaggaatt ttgctaagcg
    24841 gcggtaaacc catcaggcat tcctcttctg gcgctcaagc catattttct tcgccataaa
    24901 aatcaccacg gtcagggcaa acagaataaa cagtccggtg acaaaccaga aagtaccgcc
    24961 tgttaacatg ctgcccgccc aggaataaac aatagtcgct ggtaactgac caagcccggt
    25021 ggcgataaaa aacgagcgaa aacgtattga agtcaaaccg gcagcatagc ttacgggatc
    25081 aaaaggcaca aaaggcaata atcggcatac cagaatggtg tgtttaccat agcgggtaaa
    25141 aaagccgtcc atactattga gtaccgtttt acccgttaac ttttccacca cttcgcggcc
    25201 catcactctg gcgataaaaa agcacagcgc cgcgccggcc atcgaactgg tccacgacag
    25261 caagccgccc cagaacgcgc caaacagcga cgcattggca aaggtgatca aaaacgcagg
    25321 tagcggtgca gcaatggcct gtaaaatcat caacaagaac gagacaactg ccgccagtgc
    25381 gccgtaagac tgaataaagc gttctatacc ctgttggtcc acggcggcaa acgccgcaac
    25441 gctgcgattg ataaactcat ggacgccggg aagcagcgcc catgccagca gcatagcgaa
    25501 caacaggatg atgagggtta tacggtagta ccagattttt cgcgattgca tcttcatcat
    25561 cctttatcct gctttattcg gtgattttaa aggtaaccgt tgccagcgtg ttatccgccg
    25621 ggagaaccga ggcattgcct ttgaatttaa cttcacctcg tttttcaacc gcaccataag
    25681 tgtatgttgc attgctgacg atgccgaccg ggcagctatc cagacacacc aggcagcccg
    25741 ttttcttctc ttctgctgcc gttaaattac cgccaaagcg catgtccagt ttctttccat
    25801 tactgtcaac aatcacttca tcgaaggaat acgcttttgc cgccccttgc cagttgaccg
    25861 aaatatccag tttgctgcct gtgacatgag tcgtttcttt attatccatc gtcatgtttt
    25921 cgcccggtgt accacctgcc tctttcaggg cttcataaaa ggctttcggg gtcgcataac
    25981 ccataaacag cgatttatgt ccgttggagc catctttaaa cacaataccg tgacgagtgt
    26041 cgtcggtgag gaaacgacca ttaatttgca ccagcatggt taccgtatgg ttattggcat
    26101 cgacacgcag cggttggctg ttcgaaagtc cgtcgtattc cacttttgca gccgcattct
    26161 ctttttgatc gcagccgcta agcccggcaa cagccagtaa gcagagtgca gccagtcctt
    26221 ttttccatga cactgaataa tgttgcaaca tcgttctatc cttcattttt ggggtgagaa
    26281 agtgggctgc ggcgcgaagc agacagatcc acatgtttgt gtcgggcgta gagttttgcg
    26341 agctggacga gaataaacag tgccaggccc gccagacaga gttgtaaaat aaagcgcagc
    26401 gtaatgcctt cattggcgag atcgcttgcc atcacggtat aaataacaat accgggcagg
    26461 gttgtgagtg ccgaaataag ggtataaggc cagaaggcga ttgtggttaa tccgtaagcg
    26521 taattttgaa tattgtaagg aaacaacggg attaagcggg tcagaataag aaaatcaata
    26581 ccgttacgcg caatgccttt ttcaatggcc tgaaaggtat ggctatggcc aacgtatttc
    26641 agcagtaaat cacgtcccag ccagcgcgcc agcaggaatg agcacgagga ggccagcgtg
    26701 gcggcaatta atgatagtag tgtccctaag agcgggccaa aaactattcc tccagcgatc
    26761 accagtatgc ttcctggtaa cagaaagagg gtggcaatga tgaataacag aatatagagg
    26821 ctataaccga acaatccgct ctggcgaatg agtgtcagta agtgaggtaa atcggtgagc
    26881 agctcgaata aaccgaaagc gtggaaagcg taaatgacca gcgcaaaaat aagacaggca
    26941 aaaagaaatt tacgctcagc gttcatcgtg gagtttccta tatcaaactt gataatagag
    27001 aagtggtcag acaactaaaa acgcagtgac ggtataacaa agggcggcag gcaacaaatc
    27061 attactgaca gtttgttgat ttttctcaag gttaattctc ctgacccagt ttgagccagg
    27121 agaacagcta aattagcggc ggaaggtcgc ccagacgggg gcgtgatcgg acggtttttc
    27181 catgctgcgg atttcatagt cgatgccggt ttctacgcaa cattctgcca gcggttggct
    27241 ggcgagaagc aggtcgatgc gcagaccacg gttatcgtca aaaccttttg agcggtaatc
    27301 aaaccatgag aaacgattgg ctgtttgcgg attcgcatgg cggaaggtat caaccaatcc
    27361 ccagctcatc agtctctcca tccattcgcg ttcctccgga aggaaagaac attttccggt
    27421 acgcagccag cgcttacggt tctcttcgcc aatgccaata tccaggtcgc caggactgat
    27481 attcatatcg cccataatca gcaccggatt ttcacgcttg agttcggttt ccagatagtt
    27541 ttgcagattc tgataaaact gcgcctttgc cgggaatttt atcggatggt cgcggctttc
    27601 accctgcggg aagtaaccgt tgatcacggt gacattgccc agcggcgagg ggatttccgc
    27661 cataataatc cgccgttgcg cctcttcgtc gtcaccggga aagccgcgac gcacggcaat
    27721 cggcgtctct ttggtcagca gcgccacgcc ataatggcct ttctgtccgt gataaaacac
    27781 gttgtagccg agcttcgcca cctcttcgag cggaaacata tcgtcatgaa cttttgtctc
    27841 ctgcaggcca atcacatccg gttggtgctt ttcgacgatg gcttcaagct ggtgaggtct
    27901 ggcgcgcagg ccgttgatat taaaagagac aaatttcata gtcgctgcca tttattcaga
    27961 taaagagtgc gtggatggta gcagaatttc gctttgcgta ccgcctgttg ttaagtgatt
    28021 tgatcgatgg ttgttaatga tgtcaacgat ggcgcaaaaa atgcccgctt ttggtgcgcg
    28081 ttgcgtcaga atggcgcagt aatttccagt aaatttcgtc aatgatcaca attccagaat
    28141 tatattttac ttctgcataa cattgcgttt tttattcttt ttattcaccc acatcgcaaa
    28201 cgtattcact ttatatgcac tttaaatgca tatggtttgg ttataactcc ttgatttcca
    28261 gttagcctcc gccgtttatg cacttttatc actggctggc acgaaccctg caatctacat
    28321 ttacagcgca aacattactt attattaaca tataaataac gaattattta ctgtagaggt
    28381 cgctatgtct cagccaatta cgcgtgaaaa ctttgatgaa tggatgatac ctgtttacgc
    28441 tccggcaccg tttataccgg tacgtggcga aggttcgcgc ttgtgggatc agcaggggaa
    28501 agagtatatc gacttcgcgg gtggcattgc ggtgaacgcg ctgggccatg cgcatccgga
    28561 actgcgtgag gcgctgaacg aacaggcgag taagttctgg cataccggca acggttacac
    28621 caacgagccg gtactgcgac tggcgaaaaa attgatcgac gccacgtttg cctatcgcgt
    28681 cttcttttgt aactccggtg cggaagccaa cgaagcggcg ctaaaactgg cgcgtaaatt
    28741 cgctcacgac cgctacggca gccataagag cggtatcgtg gcgtttaaaa atgcgtttca
    28801 tggtcgcacg ctgtttaccg tcagtgcggg cggtcagcca gcctattcac aggattttgc
    28861 gccactgccg ccggatattc gtcatgccgc atataacgat attaactctg ccagcgcgct
    28921 gattgacgac tctacctgtg cggtgattgt cgaacccatc cagggggaag gcggtgtggt
    28981 gccagcaagc aacgcatttt tacaaggtct gcgtgaattg tgtgaccgcc acaatgcgct
    29041 gttgattttt gatgaagtac aaaccggcgt cgggcgcacc ggggaactgt atgcctatat
    29101 gcactacggc gtgacgcccg atctgttaac taccgccaaa gcactgggcg gcggtttccc
    29161 cgttggcgct ctgttggcaa ccgaagaatg cgccagcgtg atgaccgtgg gcacacatgg
    29221 taccacttat ggcggtaatc cgctggcctc ggcggtggca ggtaaagtgc tggatctcat
    29281 caacacgcca gagatgctta atggcgttaa acagcgtcac gactggtttg ttgagcgtct
    29341 taataccatt aatcaccgct gcggtttgtt cagtgaaatt cgcggattag gtctgctgat
    29401 tggctgtgtg ctgaatgccg attacgccgg gcaagcgaaa caaatttctc aggaagcggc
    29461 gaaagcgggc gtaatggtgc tgattgccgg tggcaacgta gtgcgttttg cgccagcgct
    29521 caatgtcagc gaagaagagg tgacgaccgg gctggatcgt tttgcggcgg cttgtgaaca
    29581 ctttgtgagc ggaggttcat catgatggtc atccgtcccg ttgagcgctc agacgttgcg
    29641 gcgctgatgc agcttgccag caaaacgggc ggcggcctga cgtcgctccc tgctaatgaa
    29701 gccacgcttt cagcgcgtat cgaaagggcc atcaaaacct ggcagggcga actgcccaaa
    29761 agcgagcagg gttatgtgtt tgtactggaa gagagcgaaa caggcactgt ggcggggatt
    29821 tgcgccattg aggtggcggt tgggctgaac gatccctggt acaactatcg cgtcggcacg
    29881 ctggttcacg cctcaaaaga gctgaatgtc tataacgcgt tgccgacgct gtttctcagt
    29941 aacgatcaca ccggcagcag cgagctgtgc acgctgtttc tcgacccgga ctggcgcaaa
    30001 gagggcaacg gctatttgct gtcgaaatcg cgctttatgt ttatggcggc ctttcgcgac
    30061 aagtttaatg acaaagtggt cgccgaaatg cgcggggtga ttgacgaaca tggctattcg
    30121 ccgttctggc aaagcctcgg taaacgcttc ttttcgatgg attttagccg cgccgatttt
    30181 ctctgtggca ccgggcaaaa ggcatttatt gcggaactga tgccgaaaca tccgatctat
    30241 acccactttt tatcccagga agcgcaggac gttatcggcc aggtacatcc gcaaaccgcg
    30301 cccgcccgcg cggtgctgga gaaagaaggt tttcgctacc gcaactatat cgacatcttt
    30361 gacggcgggc cgacgcttga gtgtgacatc gaccgcgtgc gcgccatccg caaaagtcgg
    30421 ctggtggaag tggcagaagg gcagcctgcg cagggcgatt tcccggcctg cctggccgcc
    30481 aatgaaaatt atcaccattt ccgcgtggtg ctggtgcgta ccgatccggc aaccgagcgt
    30541 ttgatattaa ccgccgcaca actggatgcc ctgaaatgcc acgccgggga tcgcgttcgt
    30601 ctggtgcgcc tgtgcgcaga ggagaaaaca gcatgacttt atggatcaat ggtgactggg
    30661 ttacgggcca gggcgcatcg cgtgtgaagc gtaatccggt atcgggcgag gtgttatggc
    30721 aaggcaatga tgccgatgcc gctcaggtcg ggcaggcttg tcgggctgcc cgtgcggcgt
    30781 ttccgcgctg ggcgcgtctc tcctttggcg atcgtcaggt aagagttgaa cgctttgccg
    30841 gattgctgga aagcaataaa gccgaattaa ccgcgattat tgccagagaa acgggtaagc
    30901 cgcgctggga agcggcaacc gaagtgatgg cgatgatcaa taaaatcgcg atatcaatta
    30961 aggcgtatca cgttcgtacc ggcgagcagc gtagtgaaat gccggacggc gcggcgagcc
    31021 tgcgacatcg cccgcacggc gtgctggcgg tgtttgggcc gtataatttc cccggtcatt
    31081 taccgaacgg acatatcgtt ccggcattgc tggcaggtaa caccattatc tttaaaccca
    31141 gcgaactgac accgtggagt ggcgaagcgg taatgcgttt atggcagcag gctggcttgc
    31201 cgccgggcgt gctgaacctg gtgcagggcg ggcgtgaaac gggtcaggcg ctgagtgcgc
    31261 tggaggatct cgacggcttg ctgtttaccg gcagcgccaa taccggctac cagctgcatc
    31321 gccagctttc cggtcagccg gagaaaattc tcgcccttga gatgggcggt aataacccgc
    31381 taattatcga tgaggtggcg gatatcgatg ccgctgtcca tctgaccatt cagtcggcgt
    31441 ttgtcactgc cgggcaacgc tgcacctgcg cccgccgttt attgctgaaa agtggaacgc
    31501 agggcgatgc atttcttgct cgcctggttg ccgtcagcca gcggttaacg ccgggccgtt
    31561 gggatgacga accgcagccg tttattggcg ggctgatttc tgagcaggcc gcacagcagg
    31621 tggttactgc ctggcaggaa ctggaggcga tgggcggacg gaccctgctt gcgccgcgct
    31681 tattacaagc agggacatcg ttgctgacgc cggggatcat tgaaatgaca ggcgttactg
    31741 gcgtgccgga tgaagaagtg tttgggccgt tattgcgcgt ctggcgttat gacaatttcg
    31801 atgaagcgat tcgaatggcg aataacactc gcttcggcct ctcttgcggt ctggtttccc
    31861 ccgagcggga aaaattcgat caactgttgc tggaggcacg ggcggggatt gttaactgga
    31921 acaaaccgct taccggtgct gccagtaccg cgccattcgg cggcattggc gcttccggta
    31981 accatcgccc cagcgcctgg tatgccgcag attattgtgc ctggccgatg gcaagtctgg
    32041 agtcggactc gttaacattg cccgcaacgc ttaaccccgg gctggatttt tccgatgagg
    32101 tggtgcgatg aacgcctggg aaatcaattt cgacgggctg gtagggctga cgcatcatta
    32161 cgcgggcctg tcgtttggta atgaagcctc cacccgtcac cgttttcagg tctctaaccc
    32221 gcgactggcg gcgaagcagg gcttactgaa aatgaaagcc cttgccgacg cgggattccc
    32281 ccaggcggtg atcccgccgc acgagcgtcc gtttattccg gtgctgcgtc agttgggatt
    32341 cagcggcagc gatgagcagg tactggaaaa ggttgcccgc caggcaccgc actggctttc
    32401 cagcgtcagc tccgcttcgc caatgtgggt agccaatgcg gcaacgatcg cgccatctgc
    32461 cgatacgctg gatggcaaag tgcatctcac cgttgccaac ctgaacaata aatttcaccg
    32521 ttcgctggaa gcccccgtca ccgaatcgtt gttaaaagcg atttttaacg acgaagagaa
    32581 atttagtgtc cattcggcgt tgccgcaggt agcgttgctc ggtgatgagg gggcggcaaa
    32641 ccacaatcgt ctcggcggtc attacggtga accgggtatg caactttttg tctacgggcg
    32701 agaagaaggc aatgataccc ggccttcccg ttatccggcg cggcagactc gcgaagccag
    32761 cgaggcggtg gcaaggctga atcaggtgaa tccccaacag gtgattttcg cccagcaaaa
    32821 cccggacgtt atcgaccagg gcgtttttca taatgacgtg attgccgtga gtaaccgcca
    32881 ggtgctgttt tgccatcaac aggcgttcgc tcgccagtca cagttactgg caagcctgcg
    32941 tgcgcgggtg aacggtttta tggcgataga agttccggcg actcaggttt ccgtgtcaga
    33001 tgcggtgtct acatatctgt ttaacagcca actgctgagc cgcgatgatg gttccatgat
    33061 gttggtgctg cctcaggagt gtcgggaaca cgccggagta tggggttatc tcaatgaact
    33121 ccttgccgct gacaacccga ttagcgaact aaaagtcttt gatttacgtg aaagcatggc
    33181 gaatggcgga ggcccggcgt gcctgcggtt gcgcgtggta ttgacagaag aagaacgccg
    33241 ggcagtgaat ccggcggtga tgatgaacga tacgctgttt aatgcgctca atgactgggt
    33301 ggatcgttac taccgcgatc gccttactgc tgccgatctg gccgacccgc aattgctgcg
    33361 cgaagggcgg gaagcactgg atgtattgag ccaattactg aatctcggtt cggtttatcc
    33421 gttccagcgc gagggagggg gaaatggata attttcttgc tctgacctta acgggtaaaa
    33481 aaacggttat caccgagcga gaaatcaacg gcgttcgctg gcgctggctg ggcgatggcg
    33541 tgctggaact gacgccatta acgccaccgc aaggcgcgct ggtgatttca gcggggatcc
    33601 acggtaatga gacggcacct gtggaaatgc tggacgcgtt gcttggcgcg atatctcacg
    33661 gtgagatccc gttacgttgg cggttactgg tgatcctcgg gaatcctcct gcgctgaagc
    33721 aagggaaacg ttattgccat agcgatatga atcgaatgtt tggcggtcgt tggcagctat
    33781 ttgctgaaag cggggaaacc tgtcgggcgc gcgaactgga gcagtgcctg gaagattttt
    33841 atgaccaggg caaagaatct gtgcgctggc accttgatct ccataccgca attcgtggct
    33901 ccttgcatcc gcggttcggt gtattaccgc aacgcgacat tccctgggac gagaaatttc
    33961 tgacgtggct gggtgcggcg ggtctggagg cactggtgtt ccatcaggag cctggtggta
    34021 cgtttaccca tttcagcgcc agacattttg gcgcgctggc ctgtacgctg gaactcggca
    34081 aagcgttgcc ctttggacaa aacgatcttc gccagtttgc agtcactgcc agcgcaattg
    34141 ctgcgctgct atctggtgag agtgtcggta tcgtgagaac gccgccgctc cgttatcggg
    34201 tggtttcgca aattactcgc cactcgccgt ccttcgaaat gcatatggca agtgacacgc
    34261 tgaattttat gccgtttgag aaaggaacat tgctggcgca ggacggggag gaacgtttta
    34321 ccgtaaccca tgatgtagag tatgtgttat tccctaatcc tttggtagcg ttgggattac
    34381 gcgcgggatt aatgctcgaa aaaataagct aatacttacc cgcagaaatc attctgcggg
    34441 tattaagtta ctttcttata aattaataca gattaatcct ggttatttgc tttatttatc
    34501 accag
//
LOCUS       DAZGAP010000025        34487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGAP010000025 DAZGAP010000000
VERSION     DAZGAP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.025"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..619
                     /gene="srlA"
                     /locus_tag="ACHLAM_001049"
                     /gene_synonym="gutA"
     CDS             56..619
                     /gene="srlA"
                     /locus_tag="ACHLAM_001049"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO7852614.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            616..1575
                     /gene="srlE"
                     /locus_tag="ACHLAM_001050"
                     /gene_synonym="gutE"
     CDS             616..1575
                     /gene="srlE"
                     /locus_tag="ACHLAM_001050"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO7852615.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1586..1957
                     /gene="srlB"
                     /locus_tag="ACHLAM_001051"
                     /gene_synonym="gutB"
     CDS             1586..1957
                     /gene="srlB"
                     /locus_tag="ACHLAM_001051"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO7852616.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1961..2740
                     /gene="srlD"
                     /locus_tag="ACHLAM_001052"
     CDS             1961..2740
                     /gene="srlD"
                     /locus_tag="ACHLAM_001052"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO7852617.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2846..3205
                     /gene="gutM"
                     /locus_tag="ACHLAM_001053"
     CDS             2846..3205
                     /gene="gutM"
                     /locus_tag="ACHLAM_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO7852618.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3272..4045
                     /gene="srlR"
                     /locus_tag="ACHLAM_001054"
                     /gene_synonym="gutR"
     CDS             3272..4045
                     /gene="srlR"
                     /locus_tag="ACHLAM_001054"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO7852619.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            4038..5003
                     /gene="gutQ"
                     /locus_tag="ACHLAM_001055"
     CDS             4038..5003
                     /gene="gutQ"
                     /locus_tag="ACHLAM_001055"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO7852620.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5000..6514)
                     /gene="norR"
                     /locus_tag="ACHLAM_001056"
     CDS             complement(5000..6514)
                     /gene="norR"
                     /locus_tag="ACHLAM_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO7852621.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     IVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6701..8140
                     /gene="norV"
                     /locus_tag="ACHLAM_001057"
     CDS             6701..8140
                     /gene="norV"
                     /locus_tag="ACHLAM_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO7852622.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8137..9270
                     /gene="norW"
                     /locus_tag="ACHLAM_001058"
     CDS             8137..9270
                     /gene="norW"
                     /locus_tag="ACHLAM_001058"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO7852623.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(9494..11746)
                     /gene="hypF"
                     /locus_tag="ACHLAM_001059"
     CDS             complement(9494..11746)
                     /gene="hypF"
                     /locus_tag="ACHLAM_001059"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO7852624.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11899..12426)
                     /gene="hydN"
                     /locus_tag="ACHLAM_001060"
     CDS             complement(11899..12426)
                     /gene="hydN"
                     /locus_tag="ACHLAM_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO7852625.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12575..13585)
                     /gene="ascG"
                     /locus_tag="ACHLAM_001061"
     CDS             complement(12575..13585)
                     /gene="ascG"
                     /locus_tag="ACHLAM_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO7852626.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13845..15302
                     /gene="ascF"
                     /locus_tag="ACHLAM_001062"
     CDS             13845..15302
                     /gene="ascF"
                     /locus_tag="ACHLAM_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO7852627.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            15311..16735
                     /gene="ascB"
                     /locus_tag="ACHLAM_001063"
     CDS             15311..16735
                     /gene="ascB"
                     /locus_tag="ACHLAM_001063"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO7852628.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16893..17363)
                     /gene="hycI"
                     /locus_tag="ACHLAM_001064"
     CDS             complement(16893..17363)
                     /gene="hycI"
                     /locus_tag="ACHLAM_001064"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO7852629.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(17356..17766)
                     /gene="hycH"
                     /locus_tag="ACHLAM_001065"
     CDS             complement(17356..17766)
                     /gene="hycH"
                     /locus_tag="ACHLAM_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO7852630.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(17763..18530)
                     /gene="hycG"
                     /locus_tag="ACHLAM_001066"
     CDS             complement(17763..18530)
                     /gene="hycG"
                     /locus_tag="ACHLAM_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO7852631.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(18530..19072)
                     /gene="hycF"
                     /locus_tag="ACHLAM_001067"
     CDS             complement(18530..19072)
                     /gene="hycF"
                     /locus_tag="ACHLAM_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO7852632.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(19082..20791)
                     /gene="hycE"
                     /locus_tag="ACHLAM_001068"
     CDS             complement(19082..20791)
                     /gene="hycE"
                     /locus_tag="ACHLAM_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO7852633.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(20809..21732)
                     /gene="hycD"
                     /locus_tag="ACHLAM_001069"
     CDS             complement(20809..21732)
                     /gene="hycD"
                     /locus_tag="ACHLAM_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO7852634.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(21735..23561)
                     /gene="hycC"
                     /locus_tag="ACHLAM_001070"
     CDS             complement(21735..23561)
                     /gene="hycC"
                     /locus_tag="ACHLAM_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO7852635.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(23558..24169)
                     /gene="hycB"
                     /locus_tag="ACHLAM_001071"
     CDS             complement(23558..24169)
                     /gene="hycB"
                     /locus_tag="ACHLAM_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO7852636.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24294..24755)
                     /gene="hycA"
                     /locus_tag="ACHLAM_001072"
     CDS             complement(24294..24755)
                     /gene="hycA"
                     /locus_tag="ACHLAM_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO7852637.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            24967..25317
                     /gene="hypA"
                     /locus_tag="ACHLAM_001073"
     CDS             24967..25317
                     /gene="hypA"
                     /locus_tag="ACHLAM_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7852638.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            25321..26193
                     /gene="hypB"
                     /locus_tag="ACHLAM_001074"
     CDS             25321..26193
                     /gene="hypB"
                     /locus_tag="ACHLAM_001074"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO7852639.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26184..26456
                     /gene="hypC"
                     /locus_tag="ACHLAM_001075"
     CDS             26184..26456
                     /gene="hypC"
                     /locus_tag="ACHLAM_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO7852640.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            26456..27577
                     /gene="hypD"
                     /locus_tag="ACHLAM_001076"
     CDS             26456..27577
                     /gene="hypD"
                     /locus_tag="ACHLAM_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO7852641.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            27574..28584
                     /gene="hypE"
                     /locus_tag="ACHLAM_001077"
     CDS             27574..28584
                     /gene="hypE"
                     /locus_tag="ACHLAM_001077"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO7852642.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            28658..30736
                     /gene="flhA"
                     /locus_tag="ACHLAM_001078"
     CDS             28658..30736
                     /gene="flhA"
                     /locus_tag="ACHLAM_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO7852643.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            30781..31209
                     /locus_tag="ACHLAM_001079"
     CDS             30781..31209
                     /locus_tag="ACHLAM_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO7852644.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(31248..31592)
                     /gene="ygbA"
                     /locus_tag="ACHLAM_001080"
     CDS             complement(31248..31592)
                     /gene="ygbA"
                     /locus_tag="ACHLAM_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO7852645.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            31878..34439
                     /gene="mutS"
                     /locus_tag="ACHLAM_001081"
     CDS             31878..34439
                     /gene="mutS"
                     /locus_tag="ACHLAM_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO7852646.1"
                     /translation="MRTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
BASE COUNT         8143 a         9477 c         9227 g         7640 t
ORIGIN      
        1 gattagatta ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat
       61 agaaaccatt actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt
      121 gtttaccggg atggtgaccg gcattcttcc gctgttgatt agtctgctgg ttatcatgaa
      181 cgcactgatt aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg
      241 taaccctgtt tcccgttacc tgctgttacc gtgcattggc acgtttgtct tttgcaatcc
      301 gatgacctta agcctgggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc
      361 cgcctcttat agctgccact caatgaatgg cctcttcccc catatcaacc caggcgaact
      421 gtttgtttat cttggcattg ccagcggtct gacaacactg aacctgccac ttggcccact
      481 ggcggtgagt tatctgctgg ttggtctggt caccaacttc ttccgcggct gggtgaccga
      541 tctgaccacc gccatttttg agaaaaagat gggcattcaa cttgaacaaa aagttcatct
      601 ggcaggagca acatcatgac gcgtattcgg atcgaaaaag gaacgggtgg ctggggcggc
      661 ccgcttgagc tggaagccac gccgggcaaa aaaatcgtct atatcaccgc cggtacccgg
      721 cctgcgattg ttgacaaact ggcacagctt actggctggc aggctattga cggatttaaa
      781 gaaggtgaac ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta
      841 cgctgcggca tttatccgaa acggcgtatt cccaccatta atatccactc gacgggcaag
      901 tccggtccac tagcgcaata cattgtagaa gatatttatg tctctggcgt aaaagaagaa
      961 aacatcactg tagtgggtga tgcgacacca caaccctctt ccgtgggccg tgactatgac
     1021 accagcaaga aaatcaccga acaaagcgat ggtttactgg cgaaggtggg aatgggtatg
     1081 ggttctgccg ttgccgtgtt gtttcaatct ggtcgtgaca ccatcgacac tgtattaaaa
     1141 accattctgc cgtttatggc attcgtttcg gcgcttatcg gcatcattat ggcttccggt
     1201 cttggcgact ggattgccca cggtcttgct ccgctggcga gccatccact gggtctggtc
     1261 atgctggcgc tcatctgctc cttcccgctg ctttcacctt tcctcggccc aggcgcagtt
     1321 atcgcacagg ttatcggcgt attgattggc gtgcagattg gtctcggcaa tattccgccg
     1381 catctggctt tacctgcact gtttgccatc aacgcgcagg cggcctgcga cttcatcccg
     1441 gtcggtttgt cgctggcgga agcccgtcag gacacggttc gcgtcggtgt cccttctgtt
     1501 ctggtgagcc gctttttaac cggtgcgcca actgtactga tcgcctggtt tgtctccggt
     1561 tttatctatc aatagaggct gaaacatgac cgttatttat cagaccacca tcacccgtat
     1621 tggcgcgagt gccactgacg ccctcagcga ccagatgctc atcacctttc gtgaaggcgc
     1681 gccagcggat ctcgaagagt attgcttcat tcattgccac ggcgagttga aaggtgcact
     1741 ccatcccggt ttgcaatttt cactcgggca gcatcgctat ccggtgaccg ccgttggcag
     1801 cgtggcggaa gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa
     1861 cgaagcggaa tttccgggca ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc
     1921 gggatcggtt ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt
     1981 catcggtggt gggcaaacct taggcgcgtt cctgtgccac ggtctggctg ccgaggggta
     2041 tcgcgtcgcg gttgtcgata ttcagagcga caaagccgca aatgtggcac aagaaattaa
     2101 cgccgaatat ggtgaaggta tggcgtacgg ttttggtgct gacgccacta gcgagcaaag
     2161 cgttctggcg ctctctcgtg gggtagatga aatctttggt cgcgtggatt tgctggtcta
     2221 cagcgccgga atagcaaaag cagcctttat cagcgacttc cagctcggcg attttgaccg
     2281 ttcgctacag gtgaatctgg tgggttattt cctgtgtgcc cgtgaatttt cgcgtttgat
     2341 gatccgcgac gggattcagg ggcgcattat tcagatcaac tcgaaatccg gcaaagtggg
     2401 cagcaaacac aactctggct acagcgcagc gaaatttggt ggcgtcgggc tgactcaatc
     2461 actggcgctg gatctggcgg agtacggcat tacggtgcat tcactgatgc tcggtaatct
     2521 gctgaaatcg ccgatgtttc agtcgctatt gccacaatat gcgaccaagc tgggtatcaa
     2581 accggatcaa gtcgagcagt attacatcga caaagtaccg ctcaaacgcg gctgcgatta
     2641 tcaggatgtg ctgaatatgc tgctgttcta cgccagtcct aaggcgtcgt actgcactgg
     2701 tcagtcgatc aatgtcaccg gcggtcaggt gatgttctga ttaacagcgg agatccgtta
     2761 aggatctccg cgagactaat aaaatgcctg atgcgctacg ctcatcaggc atacaggact
     2821 tccgccacta cattaaggaa aagttatggt atccgcactc atcaccgtcg ccgttatcgc
     2881 ctggtgtgcg caactggcct taggcggttg gcaaatttct cgttttaacc gtgccttcga
     2941 cacactatgc cagcaagggc gggttggcgt gggccgttcc agcgggcgct ttaaaccgcg
     3001 ggtcgtggtc gccatcgccc tggacgatca gcaacgcatc gtcgacacct tgtttatgaa
     3061 aggactgacc gtcttcgccc gaccacaaaa aattcccgca attaccggta tgtatgcggg
     3121 tgatttacag cccgatgtga tctttcccca tgatccacta tcacagaatg ctctatcatt
     3181 ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg cgaagttatc
     3241 attttgaaac ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc aggccgccat
     3301 tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa gaattggcgc aatactttga
     3361 caccacaggc acaaccattc gcaaagatct ggtcattctg gaacatgccg gaaccgtcat
     3421 tcgtacttat ggcggagtgg tgttgaataa agaggaatcc gatccgccaa tcgatcataa
     3481 aacactcatc aacacccaca agaaagagct gattgcagaa gctgccgtta gttttatcca
     3541 tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg tccccctgct
     3601 ctcgcgcttt aataacatca cagtgatgac caacagcctg catatcgtca atgcgctatc
     3661 cgaactggat aacgaacaaa ctatcctgat gccaggcgga acatttcgta aaaaatcggc
     3721 ctcatttcac gggcaactgg cagagaatgc cttcgagcat ttcaccttcg ataaattgtt
     3781 tatgggcacc gacggcatcg atctcaatgc gggcgtaacc acgttcaacg aggtttatac
     3841 cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg actcatcaaa
     3901 gtttggccgt aaaagcccca acgtagtttg cagtcttgaa agcgtcgata agctaattac
     3961 cgacgcaggt atcgatccag cgtttcgtca ggcgctggaa aagaaaggga tcgacgtgat
     4021 cataaccgga gagagcaatg agtgaagcac tactgaacgc gggacgtcag acgttaatgc
     4081 tggagttgca ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt gttcgcgccg
     4141 ccaatatcat cctgcactgc gaaggcaaag tggtggtttc gggaattggc aaatcgggcc
     4201 acattggtaa gaaaatcgcc gcaacgcttg ccagcaccgg cactcctgcc ttttttgtcc
     4261 atccggcaga ggcattgcac ggcgacctgg ggatgatcga aagccgcgat gtgatgctgt
     4321 ttatctctta ctccggtggc gcgaaggaac tggatctgat tattccgcgt ctggaagata
     4381 aatctatcgc gctgctggcg atgaccggca aaccgacgtc accgctgggc ctggcggcga
     4441 aagcggtgct ggatatctcc gtagaacgcg aagcctgccc gatgcacctt gcgccgacct
     4501 ccagcaccgt caataccctg atgatgggtg acgcgctggc gatggcggtc atgcaggcgc
     4561 gcggatttaa tgaagaagat tttgcccgct cccaccctgc tggagcactg ggcgctcgct
     4621 tgctgaataa agtgcatcat ctgatgcgcc gtgacgatgc catcccgcag gtggcgttaa
     4681 ccgccagcgt gatggacgcg atgctggaac ttagccgcac cggtttaggt ctggtggcag
     4741 tgtgcgatga tcaacgactg gtgaaaggcg tctttaccga cggtgattta cgtcgctggc
     4801 tggttggcgg cggcgcactc accacgccag tcaatgaagc gatgacggtc ggcggcacca
     4861 cgttgcaatc gcaaagccgt gccatcgacg ccaaagaaat cctgatgaaa cgcaaaatca
     4921 ccgccgcacc ggtagtggat gaaaacggca aactcaccgg cgcaattaac ctgcaggatt
     4981 tctatcaggc cgggattatt taatccttca gccccagacg tttcgccagc cgatgcaggt
     5041 tggcgacgtc ggtttccagc atccgcgcac aggcggccca gttgtgatga ttttgcgcca
     5101 gtgcctgacg aatggtttca cgctggaacg cttctgtcgc ttcacgcagg ttttgcttaa
     5161 cgataggcac cgccgccgct tctggcggcg gcaacgtcac ctcaggaaaa gcaaaatgtt
     5221 gcgcctcaag aatcacttca tcgccgctgc gagtggctct cgacagtact accgcccgat
     5281 gaatagcatg ttccagttcg cgcacgtttc ccggaaaact gtagtgttgc agtaaatttc
     5341 gcgccccggc acttaatacc acgcgggaga gccccagccg caaacgacac tgctcgcaga
     5401 aataccccgc cagcagaatg acatcatcgc cccgctcacg cagcggcggc accgaaagtg
     5461 gaaacacgct caggcgatga aacaaatcgg cgcggaatcg ccctgccagc acctcttcgc
     5521 gtaaatcgcg gttagtcgcc gccagcacgc gcacatcgac ccgcaaactg cggtcatcgc
     5581 caacgcgctg aatatcgcca tactgcaaca ccctcagcag cttggcctgc aatgccaacg
     5641 acaactcgcc gatctcatcg agaaacagcg tgccgttatc cgccatttca aacttcccgc
     5701 tgcggttact gatagcacca gtaaacgccc ctttcacatg cccgaacaac tcactttccg
     5761 ccacactttc cggcagtgcg gcacagttga gatagaccag cggattcacc gcccgtggcg
     5821 aggcttcatg aatcgctttc gccaccagct ccttaccggt tcccgtctca ccgctaatta
     5881 aaacgttgag atcggatgcc gccacaatct caatctcttt tttcaattgc gtcatgcctg
     5941 gggacaaacc aatcatctgc gtctgtttca ccgcttcaaa cggcgcggca tcgcctggca
     6001 gcatattctg gctttccagt tgttcaatca gtaacgcatt acttaacgct cccgccgcca
     6061 gtgcggcaat cagccgtagc tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc
     6121 cgtcgagcgt caaggcaccg atcaggtttt gcccggcaaa caacggcaaa ccaacgcagg
     6181 cgtgaacctt cagactctcc tgccctggaa tcaaaccgtc ataaggatcg ggcaattcgc
     6241 tgtctgcggg aaagcgcacc acatcacccg cgcgggcaat cgcttccagc cgtggatgac
     6301 cttccagcgc aaagcgtcta ccgagtacat ccttcgccag cccgtcgatg gcaagcggaa
     6361 taaactgccg cgagtcgtaa cgtagcaacg cagacgcatc gcactccagc acctgacgca
     6421 gcgtggtgat caggcgctga aaacgatcct gatgaccaat cccacgctgc aattcgatgg
     6481 cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca atttgactat
     6541 agatattgtc atatcgacca ttttattgat agtcattttg actactcatt aatgagcaca
     6601 attttattta tagagtaaaa acaattagat aaaaaactgg cacgcaatct gcaatcagca
     6661 agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg tggtgaaaaa
     6721 taacattcat tgggttggtc aacgtgactg ggaagtgcgt gattttcacg gtacagaata
     6781 taaaacgctg cgcggcagca gctacaacag ctacctcatc cgcgaagaaa aaaacgtgct
     6841 gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc gtaatgaaat
     6901 cgatctgacg gatatcgatt acatcgtaat taaccatgct gaagaggacc acgccggggc
     6961 gctgaccgaa ctgatggcac aaattcccga tacgccgatc tactgtactg ccaacgctat
     7021 cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtgg tgaaaacagg
     7081 cgacacgctg gatatcggca acggcaaaca gctcattttt gtcgaaacgc caatgttgca
     7141 ctggccggac agcatgatga cttacctgac aggcgacgcg gtactgttca gtaacgatgc
     7201 tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc agacggagct
     7261 tttcgagcag tgccagcgtt attacgccaa tatcctgacg ccgttcagcc gcctggtaac
     7321 gccgaaaatt accgagatcc tgggctttaa cttaccagtc gatatgatag cgacctctca
     7381 cggcgtggta tggcgcgata acccgacgca aattgtcgag ctgtacctga aatgggcggc
     7441 ggattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata atacccgcat
     7501 gatggccgat gctatagccc agggtattgc ggaaaccgac ccacgcgtag cggtgaaaat
     7561 tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc gctcaaaagg
     7621 cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg ccaaaaatcg ccgggctggt
     7681 ggaggagatg actggtttac gctttcgtaa caaacgcgcc agtgctttcg gctctcacgg
     7741 ctggagcggc ggtgcggtgg atcgtctttc cacacgcctg caggatgcgg gtttcgaaat
     7801 gtcgcttagc ctaaaagcca aatggcgacc ggaccaggac gctctggagc tatgtcgtga
     7861 acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga gcacggtgaa
     7921 tacggtggtt aaagaagaaa cctctgccac cacgacggct gacctcggcc cacggatgca
     7981 gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc
     8041 gccaggaacg ccgtggagtg aagttccgga taacttcctg tgcccggaat gctcccttgg
     8101 caaagacgtc tttgacgaac tggcatcgga ggcaaaatga gcaacggcat tgtgatcatt
     8161 ggttcgggct tcgccgcccg ccaactggtg aaaaatattc gcaaacagga cgcctgtatt
     8221 ccattaaccc tgattgccgc cgacagcatg gatgagtaca acaaaccgga cctcagccat
     8281 gttatcagcc agaggcaacg tgccgatgac cttacccgcc agacggcggg tgaatttgcc
     8341 gagcagttta atctgcacct gtttccgcac acctgggtaa cggatatcga tgccgaagcc
     8401 catgtggtga aaagtcagaa taatcagtgg caatacgaca agttagtgct ggcaaccggt
     8461 gccagcgcct ttgtcccgcc agtgcccggg cgtgagttaa tgctgacgtt aaatagtcag
     8521 caagagtatc gcgcctgtga aacgcaactg cggaatgccc gacgcgtgtt gattgttggc
     8581 ggtggcttga ttggtagcga actggcgatg gatttttgtc gtgcaggcaa agcggtcacg
     8641 ctaatcgaca acgctgccag tattctggca gcgttaatgc caccggaagt aagcagccgc
     8701 ttgcagcatc ggttgacgga gatgggcgtt catctgctgt taaaatctca gttgcagggg
     8761 ctggaaaaaa cggattctgg cattctggca acgctggacc gccagcgcag catcgaagtg
     8821 gatgcggtaa ttgccgccac cggactgcgc ccggaaaccg ccctggcacg acgcgccggg
     8881 ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa tgccgatatt
     8941 tatgcgctgg gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct ccagccgatt
     9001 caacttagcg cgatggtgct ggcaaaaaat cttctcggca ataacacgcc gctgaaactc
     9061 ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc tgcatctggc aggcgaaacc
     9121 cagcgtcagg atttacgctg gcaaattaat accgaacgcc agggaatggt ggcgcgcggt
     9181 gttgatgatg ccgaccagct tcgcgccttt gtggtcagtg aggatcggat gaaagaggcg
     9241 tttggattgt tgaaaacgtt gccggtgtag gcgagctaca gtgccccaaa tgtcggatgc
     9301 ggcgctgacg cgtcttatcc gacctacagg gaacgcatgt gttgatcaca taatacgttt
     9361 atgtcgcatc ctgcactgtc ccaaaatgtc ggatgcgacg ctggcgcgtc ttatccgacc
     9421 tacggggacg cacgtgtagg ccggataagg cgtttacgcc gcatccggca atggtgtcca
     9481 aatgcaacac gctttatccg ttctggactt cacccgctaa ccagcgcgcc gcagcaataa
     9541 ccccctgccc cagagacaat ccaccatcgc ccgccggtaa actctgcggg aagagcaacg
     9601 taaaatcagc aagataatgc gccagacgtg cacgcagcaa acggttatga ataactccgc
     9661 cgctaaatac cagcgtagtg ataccccgca tcgtggcctg ctcacgcatc aacgcggcaa
     9721 aaccctgcgc cagcgcatca tgaaacgccc acgcgcgttg attaaccgga gcctgccagc
     9781 tcagccactg ctgccagaaa gtggcgagat ccagttgatt gtccaccagc ggcatcgtca
     9841 ccggatgcgt cactccgtcg cacgaagctg cgagcgcctc cagagcacaa gccgcttcac
     9901 cttcataact taacgtggct ggcgcacagc ccagtcccgc cgccacagca tcgaacaaac
     9961 gcccacacga tgacgccagc ggcgcgttaa ttccacgctc aatggcccgc gccagcacgc
    10021 tccagttttg ctgttgcaca cttgccgttt cagggtaatt ctgccactcc ggcacaaagc
    10081 gcaggcactg cgccagcagg ttacgccacg gctgcttcgc cgccaaatcg cccccagcaa
    10141 gcgccactgc aggcaagccg cccaggtgct ggcattcgcg atagttcacc cgcaggcact
    10201 cgccgcccca caaagcgccg ttctccccca taccgatacc gtcgagcgtc aacgcaatga
    10261 catcaccgcc atccagtggc cagtgatgtt ctgccagaca cgccgccgca tgagcatgat
    10321 gatgcagcac cgtttgcgtc ggcagattca tctcacgcgc ccactggctg gagacatagc
    10381 ccggatgcgc gtcatgcaca acgtattgcg gggtgaaatc gtagatgttt tgcatcaggc
    10441 gtaacgcttc gcgccactgc atctggatgc catcgtcact taaatcgccc agatgctggc
    10501 tcaacaccgc ttgttcaccg cgcaccaggc agaaggtatt tttcagatcc gcgccgagac
    10561 acagcacagg cggaacattt ttaaagcccg gaggcaaagc cagcgcatcc ggcacatacc
    10621 cccgcgaacg gcgcagcatt tcgccgcttt cgcgcaccac cgaatcatcc atccgctgca
    10681 cgatgtcgcg gttatgtatc aagaatccgt cggcaatgcc ctgcaaatcc gccagcgcct
    10741 gttcgttgct gatagccggt ggtttaccgc tcaggttgcc ggaggtcatc accagcgggc
    10801 attgcagttc ctgtaacagc aaatgctgga gcgggttcgc aggcaacatc accccaactt
    10861 cgttaaggcc aggggcgata tcatcacaaa gctcaggaac gtatttttta tccaccagca
    10921 caatcggcgc ggcgggtgtg gtaagcaact gacgggcagc gtctggtaaa ccttctgcca
    10981 ctggcaacat aaccgccagc ggtttcgccg ggcgatgttt gcgcgcccgc agtgtcgcca
    11041 ccgcgttact gttacgcgca tcgcaggcga gatgaaatcc gccaatccct ttgatggcga
    11101 caatgttgcc catttttaac tgtgcgatag ctgcctgtaa tgccgcctct tgttctgcat
    11161 gttcaccatg acttacccat tcaagatggg ggccacactc cgggcaggcc accggctggg
    11221 cgtggaagcg acgatcgagc gggtcacggt actctttgtc acaggccgga cacagcggaa
    11281 acgccgccat cacggtaaac gggcggtcgt aaggcatggc gcgaataatg gtgaaacgcg
    11341 gaccacagtg agtacagttg ataaacggat aacgccagcg tcgttcgcct ggggtattca
    11401 tttcggcaag acaagcaggg caagtcgcgg catccgggac aatttgcgta ttcatgacgc
    11461 cacccgcgct ctggcggatg gtgaactcag tgggcagttg tgaccagata aacggctcac
    11521 gctcgacgct atcaatacgc gccagtggcg ggcagtgctg atgcaactgc acaagaaacg
    11581 tttccggatc ttccagcagc cggacttcta cgccatcgcc gtcattacag acatcgccgt
    11641 gaagatttaa ttgctgtgcc agttgccaga caaacggacg aaaaccgacg ccctgcactt
    11701 tgccacgaat acgcagttgg acaccgcaag atgtgttttt tgccattgag ttattcccgc
    11761 catcatgaat tgcgtaaccc gcccagccgg acacgacagc gtcgcatccg gcagtcacag
    11821 gtcggcgata ctgcccgctt cgtattctac gaatatttcc gggaattcct ttgatgccag
    11881 aacagttcag taagattttt agaacatcag cgccgtacgg cgacgttttt ctgcgctcag
    11941 ttgttcaagt ttattacgat cgacacaaat cagcgcatgg gtcgggcaag ccgccataca
    12001 cgccgggccg tcttcacgat ggttgcacag gtcgcattta ttggcttcgg ctttgtcagc
    12061 ccgtacattc agccccgcgc cgctgttgcg aatcaccgga cgtaccacca cttccatcgc
    12121 gccatacggg caagccacaa cgcaggtttt gcaaccaata caacgttcct gcatcacatg
    12181 aacaaaccct ttatcacgac tgatagcacc attcggacag acgttagcgc acggtgcatc
    12241 ttcacactga cggcaaactg tcgccgtgga aatgttcaca cctttaatga catggattcg
    12301 cggtaaaaaa gtttccgggg tcagcgatgc acagtcctga ttttcctgat gagaaaccac
    12361 gcacgccact tcacaggtac ggcaaccaat acatttactc gcgtcagcaa tgatgaaacg
    12421 gttcatcaaa ttctccagca atgacagtta atgcgccgat acattcacaa atcatgccag
    12481 tttttaattt actgttattt aaagaaatta aattctgtaa tgcaggaaaa acgatgtcat
    12541 cgacactagt gacgatgaca tgtgatgaca atgtttatcg cgaaagagca atgagggagt
    12601 cgcggcggat cagttttccg ctgaaggttt tcggcggtgt gtaagcacca ccatcgagca
    12661 taaatatcag ccgcccaatt atttcctgaa tcatctcagt caccggaatt tttacgctgg
    12721 agagcgccgg aacggtgtag ggggcaatag cgatatcatc gaatccgata actgacacct
    12781 gctctggcac cgctacgccg cgctcgtgta acgctttcat cgcacctatc gccatatcgt
    12841 cgttactggc aactaacgcg ctaaatttag ccccacgttc gagcaacatt tctacccctt
    12901 cggccccgct ggcaggcgtc catttaccgt tagcgataag tttttcattg agcgcaatac
    12961 catgctgcgc cagcgcgtct ttatacccgg caagacgttc aacgctggtg ggggaatcca
    13021 tcgaaccggt aaggaaagca atctcctgat gtccggcgtt tatcaactct gccacggcgt
    13081 taaagctggt ctgtttatga tcgcaccaga cgctatggct gctgtttttg cgcaggcggc
    13141 gattaagcac cattatcggc tgactgtgtg cgtcaatgat gtcatcaatc tcatccacgc
    13201 ttaaaaaacg cgggtaaatc ataatggcgt cgcagcgcaa atccagcaga tactgaatcg
    13261 cctggcgctc ttcttcggcg ctgtgtttac catctgccaa tagcaactgt cgccctttct
    13321 cttccgccat tcgcgcggca tgaaagagta attcactaaa ataaatgccg tggtaaagcg
    13381 tgttggtcac gaccagcccc agcgtctgag tactcttcgc cgacagattg cgcgccagca
    13441 agtttggacg gtaaccgctc tcttctaccg cctgaaacac gcgatcttta gtctcctggc
    13501 tgacgtagcc attacctgaa agcacgcggg aaacggtcgc ttttgaaacc ccggcgcgct
    13561 tcgccacttc cagcatcgtc gtcatcattt tcatcccttt acacgcaatc aacgcagtgt
    13621 actgcaccgt ttgccgattg tccttgcgca atcggcggga aaaatattca ggtgaccggt
    13681 ttcacaaata taaaaaatga acaattaact ctcttgctta ttcagtgaca actattcatg
    13741 attttgtgaa accggtttcc taattccgtt tcagcatcgg catttttccg tcacgtcgac
    13801 tgataacaac tacatctacc ctactgataa caggataaaa tccgatggcc aaaaattatg
    13861 cggcgctggc acgctcggtg atagcggcac tgggcggcgt tgataacatc tcggcagtca
    13921 cgcactgtat gacgcgtttg cgctttgtta tcaaagatga ccagcttatc gacagtccga
    13981 cgttgaaaac catctccggc gtgctcggcg tggtacgtag tgacaaccag tgtcaggtga
    14041 ttatcggcaa taccgtttca caagcctttc aggaagtcgt cagcctgctg ccgggagata
    14101 tgcagcccgc acagcccgtg ggtaaaccca aacttacgct acgtcgcata ggtgcgggga
    14161 tcctcgatgc gctgatcggc accatgtcac cgctgatccc ggcgattatc ggcggatcga
    14221 tggtcaaact gctggcgatg atcctcgaga tgagcggcgt gctgacaaaa ggatcgccga
    14281 ccttaaccat tctgaatgtg attggtgacg gtgctttctt cttcctgccg ctgatggtcg
    14341 cggcttcggc cgccatcaaa tttaaaacca atatgtcgct ggcgattgcc atcgcgggtg
    14401 ttctggtaca tccgagtttt attgaactga tggcgaaagc ggcccagggt gaacatgtcg
    14461 aatttgccct gatcccggtc accgcggtga aatacaccta cacggtgatc ccggcgctgg
    14521 tgatgacctg gtgcctgtca tatatcgaac gctgggtgga tcgcattaca ccggcggtga
    14581 ctaaaaactt cctcaagcca atgctgattg tgttgattgc cgcaccgctg gcaatcctgc
    14641 tgattggccc gattggtatc tggatcggta gcgccatttc ggcgctggtt tacaccattc
    14701 atggttatct gggctggctt tcagtcgcca ttatgggcgc gctgtggcct ctgctggtaa
    14761 tgaccgggat gcaccgcgtc tttacgccaa ccatcattca gaccattgcc gaaaccggca
    14821 aagaagggat ggtcatgccg tcagagatcg gcgctaacct gtcgctgggc ggttcatcac
    14881 tggcagtggc gtggaaaacg aaaaacccgg aactgcgtca gacggcgctg gctgcggcgg
    14941 catcagccat tatggcgggg atttccgaac cggcgttata tggcgtggcg atccgcctga
    15001 aacgtccgct tatcgccagt cttatcagcg gttttatttg cggcgcggtt gccggtatgg
    15061 cggggcttgc cagccactca atggcagcgc cggggctatt taccagcgtg cagttcttcg
    15121 atccggcgaa tccaatgagc atcgtctggg tgttcgccgt catggcgctg gcggtcgtgc
    15181 tgtcgttcat cctcacactg ttgctcggct ttgaggatat tcctgttgag gaagcgactg
    15241 ccgaggcgcg aaagcatcag agcgcacaac cgaccgtcgc caaagaagta agtcttaatt
    15301 gaggatgaaa atgtcagtat ttccagaagg ttttttatgg ggcggcgcgc ttgccgccaa
    15361 ccagtctgaa ggtgcgtacc gggaaggtgg caaagggctg accaccgtcg atatgatccc
    15421 acacggcgag catcgaatgg cggtgaaact ggggctggaa aaacgttttc agttgcgcga
    15481 tgacgagttt tatcccagcc atgaggcgac ggatttttat catcgttata aagaagatat
    15541 cgccctgatg gcagagatgg gattcaaagt gttccgtacc tcaattgcct ggagccgcct
    15601 cttcccgcag ggcgatgaac tgacgcccaa ccagcagggc attgcttttt accgttctgt
    15661 ctttgaagag tgtaaaaagt acggtatcga accgctggtc acgttgtgcc acttcgatgt
    15721 gccgatgcat ctggtcaccg aatatggctc ctggcgtaac cgcaagctgg tggagttttt
    15781 cagccgctac gcccggacct gctttgaagc atttgatggt ctggtgaaat actggctcac
    15841 cttcaatgaa atcaacatta tgttgcatag cccgttctcc ggcgcgggtc tggtgtttga
    15901 agaaggcgaa aatcaggatc aggtgaaata tcaggctgcg catcaccaac tgattgccag
    15961 tgcattagcc accaaaatcg cccatgaggt taacccgcaa aatcaggtgg gctgtatgct
    16021 ggcgggcggt aacttctacc cttacagttg caagccggaa gatgtctggg cggcgctgga
    16081 gaaagatcgg gaaaacctgt tttttatcga tgtgcaggcg cggggcgcgt atccggctta
    16141 ctctgcccgc gtattccgcg aaaaaggggt aaccatcgac aaagcaccgg gcgatgatga
    16201 gatcctgaaa aacaccgtcg attttgtctc tttcagctat tacgcctcac gctgcgcctc
    16261 ggcggagatg aacgccaaca acagcagtgc ggcgaacgtg gtgaaatcgc tgcgtaaccc
    16321 atatctacag gtaagcgact ggggctgggg gattgatcca ctcggtctgc gtatcaccat
    16381 gaacatgatg tatgaccgct atcagaagcc gctgtttctg gtggaaaacg gcctcggcgc
    16441 aaaagatgaa cttgctgcca atggcgagat taacgatgac tatcgcatca gctatttacg
    16501 cgaacatatc cgcgcaatgg gcgaagcgat tgcagatggc attccgctga tgggctacac
    16561 cacttggggc tgtattgatt tggtttccgc ctcaacgggt gaaatgagca aacgctacgg
    16621 ttttgtctac gtagaccgtg acgacgcagg caacggtacg ctggcgcgca cgcgtaagaa
    16681 atcgttctgg tggtataaaa aagtgattgc cagtaatggt gaggatttag agtaagtaac
    16741 agtgccggat gcggcgtgaa cgccttatcc ggcctacatg tcgcacgaac caggtaggcc
    16801 cgataagcgc agcgcatcgg gcaatctggc gtttgctatc agttctgatt ccctcatcaa
    16861 gaacatccct gtcctgattc cttactgaaa aactactctt cttccaccgc taactgcgcg
    16921 aatccgccat tcccttccca gccttccagt cgttgataaa cggtttctac cgcatcttta
    16981 atcggctggg tcatcgggta gtaaaagccg acgatatccg gctggatgcc gaggaaaatc
    17041 acttcgccaa tgtcttcttt taactggtcg ataaggtagt tcaacggcat gttatgggta
    17101 gtcatcataa acatctcggc gatatcatcc ggatcgatga tgcggatctc gccggggttt
    17161 aaccccatat ccgtggcgtc gacaatcagc agccgtgtcg ggcgcagttc gcggatggcg
    17221 acgatgtcgt tttccggagc gctaccgccg tcaatcacca cccagttacc tttcggcgcg
    17281 gcggcgcact tttccgccag cagcgggcct gcgccatcat cgcccatcat gctattgcca
    17341 acacaaagta aaacgtcagt cacgcagtct cctcaccatc aaatagatgg cgttttcctg
    17401 atgaatatca tgcagcatac ttaacagcat tgtactccat tctttctgct gcgaagtttg
    17461 cgctgcccgg gctttatcga atgcgttagc cagcatcggc acatggttaa tgtcgatgac
    17521 gatctcacca tatttcggca cgccttccat tttgcggcgg gcttcgctgc ccgcctccag
    17581 agtggcaatc catgccagat agtcatccca cgggcaggta agcgccgctt ccaggcaatc
    17641 gataacccca aggtggtgac caatcgccag gctgtaatag accacctgct gcgcctcggc
    17701 gggcgtagca tcgttctcat caataaattt acggctcagt tgactgaaca ccaccttttc
    17761 actcatcgga tacgcgcctc ttcaacaaca tgattcagat ggctgacaat ctcgttcaga
    17821 cgcggatcgt tttccgcttc cagccagcgt gcaacctgtt cttcgccctg ccctaactgt
    17881 gtaaggtaat catcggcaat ctgacgaccg taacgataac ccgccaggcg gcgtgcttcg
    17941 cgatccactt tcacacgcag cggctgcacc atatcgccat gcaggatctc cgccggttgt
    18001 tcatcctgct cacccggccc acgggcgtga attttctgct ccagcaggcc gagcgccatt
    18061 gcaaagccgt atagcgtagc ggcaggcgtt ggcgggcagc cagggatgta gacatctacc
    18121 gggacgattt tatccgtacc gccccacacg cagtagagat cgtggaagat cccgccactg
    18181 ttaccgcagg caccgtagga gatacagatt ttcgggtccg gcgcggactg ccacgcacgc
    18241 agcgcagggg atcgcattgc acgggtgacc gcgccggtaa acagtaaaat atccgcatga
    18301 cgcggtgaag gaacgacttt aatgccgaag cgttctgcat caaacagcgg cgaaagcgtg
    18361 gcgaaaattt cgatttcgca gccgttgcag ccaccgcagt ccacgcggta aacgtaggca
    18421 gaacgtttga tttttttcag taacgacgcc ttcatgctgg cgatggattc atccaccgtc
    18481 atcgggaccg gaatgccgtt ggcgtcacgg gggcctaata aattgctcat cagatggcct
    18541 ctttcatatg gcgagtcagt tcaatacggt cggacggcac caggcatttc tggcgcttac
    18601 attccgggca agtctcaaag ctttcgcggt ggttttccgc gcggctgtca ccattgtgct
    18661 taagcagcgc aatggcgtag tcgatctctt tctggacggc gaaaggacga ttgcagacgc
    18721 ggcagttgca cagcgcgaag cgggactgtt ggaggaagtc ttctttcttc cacaccgcca
    18781 gttcgtactc ttgcgacagt ttgatcgccg ccgtcgggca aacttcttcg cagcgtccac
    18841 agaagatgca gcgcccgaga ttaaactccc aggccagctc tcctgtggcg aggtcggttt
    18901 caaccgttaa ggcgtttgac gggcaggcat tgacgcaggc cgcgcagccg atgcactgct
    18961 gcgggttctg ctctggctta ccacggaagt ttttatcaac cgcaatcggc tccagcggat
    19021 aagacgaggt cgccgtgccg gttttgatga cttttttgat aaaggtaaac atggcgattc
    19081 cttatttcag cggcgagttt ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca
    19141 ccactttgct cttcttctta cgcacatcga ccacggtcat gcggtcggta caggagtagc
    19201 aagggtccag gctaccgata atcagcggcg catcggaaac ggtgttgccg cgcagcatgt
    19261 agcgcagggt cggccagttc gcgtaggtcg cggcacggca gcgccagcgg tacagcttct
    19321 ggttgtcgcc ggtcatgctc cagtggatat cgtcgccgcg cggcgcttcg gcaaagccca
    19381 gcgcaaaacg gtgcggaatg taggtaaaac cttctaccat caacggaccg cccggcaggt
    19441 tatccagacc gtagtcgatc atgttcagcg cggtatagac ctcgttgatt cgcactttca
    19501 gacgggaaat aacgtcgcag ccctgctcgc tgtggacttc cattggcagc aggccataac
    19561 cgacaaacgg gtgatcggcg cgagtatcgc gggcgtgacc gctggcgcga accatcgggc
    19621 caacgttact gaaatcgcgg gcgatttccg ggtccagacg accaatgccg acggtgcgct
    19681 gttccatgtt cggtgtgctc agcagcacat ccaccagctc ctgcacttca cgacgcatct
    19741 gttgtgccag ctggcgggtc tggatcatgt cgtctttcag cagatcgcga cgaatcccgc
    19801 cgatcaggtt caggccgtag gttttacgtg caccggtaag gatctctgcc attttcatgg
    19861 aggtttcacg cacgcggaag aactgcataa agccggagtc gaagccggta aagtgacagg
    19921 cgaggccgag attgagcaga tgcgagtgca ggcgttccac ctccaacaga atggcgcgga
    19981 tcatctgcgc acgttctggc acctgaatac ccatcgcgtt ttccaccgac gtggtgtagg
    20041 cggtgctgtg agcaaagccg cagatcccgc acacacggtc agagaggaag gtcacttcgt
    20101 tgtaacccat acgggtttcc gccagttttt ccatgccgcg atggacgtag aacaggcggt
    20161 agtcggcgtc gataatgttt tcaccatcga caaacagacg gaagtgaccc ggttcgtcag
    20221 aagtgacgtg caggggacca atcggcacga cgttgttttt cttgtcgccc agttcgttga
    20281 tgaactcgta ggtttcagca tcggtagtcg gtgccggacg ctgacgataa tccatgctgt
    20341 ctttacgcag aggataaagt tcatccggcc agtcatccgg cagcaccaga cggcgttcat
    20401 ccggcagacc aaccggaatc aaaccgtaca tatcgcgcac ttcacgctcg ccccacactg
    20461 ccgccggaac gcgcggcgtc acggaaggat actccggttt gttggcgtca acttcgacgc
    20521 gcaccgttac ccaacactta gtccccttct ccatcgacag cacgtaataa acggcgtaat
    20581 gaccattcag tttgcgttcg tcgttaccaa acagcaccga cagccagccc ccctgcttgt
    20641 agtagagaaa ctccaccact tccggcaggt agttcacctt cacggtcacg gtcagctgat
    20701 ctttggtctg ccaggcgtgg tccagcacga cgcccggaaa tgcctcattc agcgcggcga
    20761 gataatgttg acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag
    20821 caaggagacg aacgctaaaa atgcaaagcc aaaaccagcc caggtaatgc gtggagtaat
    20881 atcaagacgc aaacgggcca tgctgttttc gaacagcgcg ataaccagta cgccgaccac
    20941 cagtttgacg atggcaatca ccagcgccag cagcagccca ccgacggtga aggtttccat
    21001 ttgtccccac ggaataaaca ccccgacgaa catctgcaac accaccagct gtttcaggct
    21061 gatgccccat ttcatgacgc caaagccgct gccgctatac tcagagagcg gaccttcctg
    21121 caactcctgc tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaagtggc
    21181 gaacgcacag gcacaaagcg ccagcaccag cgggatgctc tggctcagtg gccagtgata
    21241 aacggtgtcg gtgatgttgc tgatgttggt ggaaccggca acctgtgcgg cgacccacag
    21301 accaagcagc agcatcggtt caaccaacac gccaagcatc gcttcacggc tcgcgccgat
    21361 agcggtaaac gggctaccag tatccagacc agaaatggca aagaagaaac gcgcgatggc
    21421 gaagagatac agtaaggtga tcaaatcgcc cagttgcggc agcggagagc cgacggtcac
    21481 caccggcagc gcagtggcga tagtcagcat gacgcccacc atcacatacg gcgtcaggcg
    21541 gaacacccag ccggaggcat ccgggccaac gctctgacgc cccagcagtt tgataatgtc
    21601 gcgatactcc tgcaacacgc ccggcccacg acggttatgc aagcgggcgc gcgctacgcg
    21661 ggtaataccg gagagcagcg gcgcaacggc aaataacacc agcgcctgaa ttaacggata
    21721 taaaacactc attctcaggc tcctcgtgaa acaataatca ccaccagtac cgccagttca
    21781 accagcgcca tccggcggaa cagcaacgca ctcccctcgc actgccagcc cggcaccagc
    21841 gacaccggat tcagccattt gcgtagtttc agcaccggcg caaacgcctg tttcaccggc
    21901 atggcaaaac cgtgagcggt aatcaccatt gatttttcat gatcgtagcc gcacacccag
    21961 gccgcgccgc gggaacgcga cggcaaacga tcgcctttgc aaatcgccat aatgatgaat
    22021 ggcagcagcg ggcaggcaat cagcagcaac gtgatcatgg gttgagaaac ggtggtgtta
    22081 gcaggctcca gcggcagagg tacagcagca gagagcatcg gcagtagcca cggcgcagca
    22141 acaccgccaa ttacgcagca aatcgccagt gccactacgc ttacgctcat caggagcggc
    22201 gcacaggtgg cgttttcggc ctctttggta cgcggcgcgc cgaggaaagt aacgccataa
    22261 actttcgcca tacacatcac cgccagcgca ccggtaattg ccagccccac ggcgagcagc
    22321 ggcccgagaa gacgggcaac aaacgcgcca ctattgctca gtttgaagaa ggattgatag
    22381 ataacccatt ccccggcaaa cccgttcagc ggcggcagcg cagccattgc catcagcccg
    22441 actaacatgg cgatggagat aaccggcatt ttcttgccaa taccaccgag tttttcgata
    22501 tcgcgatgac cggtacggaa ccagacgctc cccgcaccaa ggaacagtac gctcttgaac
    22561 aggctatggt taagcagatg gtacagacca ccaaccagac caagggcaat cagcgccggt
    22621 tgttcgagcg cgatacccgt tacgccagcg cccagcccca gcaggatgat gccgatattt
    22681 tccagggtgt ggtaagccag cagacgttgg atattatgtt ccatcaacgc atacagaccg
    22741 ccgacgaatg cggtgatcat gccgagtacc agcagtgcga tcccccacca cagcggtgca
    22801 ttaccgccca gtagtgacag agttaaaatg cccagcaggc caattttcat gactaccgtg
    22861 gaaaacagcg cggcagctgg cgcagaggcg ttggcatgtg cctgtggcac ccagccgtgc
    22921 agcggaataa tcccggccag caggccaaag ccaatcacac cgagcagcca gatatcggaa
    22981 ccgagcggca gctgttgcat acgcatatcc agcaggcgca gatccagcgt gccgtaacgc
    23041 tgccacagca gccagcaggc aatcgccagc agcagagtgc caagacgccc cagcgcaaac
    23101 cacagtttgc cctctttgct gttgctggtg aggaacaccg cacacagggc catgatttcg
    23161 gccattacca cgaacatgcc gaggttgctg gcgatgacgg cgcagacggc ggcagccatc
    23221 aacatattga tctgcaagcc gttgcacttc acctgcgcgt ggcgatgcca gtcaatgttg
    23281 tagaggctga caaacagacc gcataggccg agcgtaatca gccaaatcgc attaagcgga
    23341 gagatttgca catcgtagct taccagcgac agcgcaccgc tcacgccaac cgcgccagtc
    23401 agcactgtga agcccgcggc tgccgtatac aggctaccaa ccgcgccgcc aattccggct
    23461 atccagccgc ttagcgcctt ttgaaaagaa aagagaaatg ccagaacagc ggcggcgaca
    23521 aaccacgcca cgccgctatt gatcagggaa attgcgctca tttagcctct ccactttgag
    23581 cctgctgaaa caaggtgaga tcgccaaaat ccgtgttaaa agtcagctca cgcttacgtt
    23641 tgctgacgcg ggcgatatcg gtgttatcca ccagatgcag ggctttagtc gggcacatcc
    23701 gcacgcaggc cggaccttgt tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc
    23761 gaatacctgg cacccagtca agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg
    23821 gcgctttcgg ggtattggcg tttgccggaa tatccagcgg acggctgccg gaaaattcaa
    23881 ttgcgccaaa cgggcaggcg atgccgcaca gtttgcagct tacgcacaga ctttcattca
    23941 actgcacggc cccatcaacg cgggtgatgg cgttaaccgg gcaaaccacc gcacagggcg
    24001 catcttcaca gtggtgacag agctgcggcg cagattcttt ttcattgagc atcactcgca
    24061 ggcgcggcat tgattgcagg ccgtgctggc gatgcgtctc tgaacaggcg gcctcacagg
    24121 tgtggcagcc gatacagagc gtggagtcag caattacaaa acgattcacc aggcattcct
    24181 caggtgattg tcatttttga cgaaaacatg ccgatgaaat gtcattttcg acactcatcg
    24241 acacgcccat ccccaaacag gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct
    24301 gccgggcttt gtccctttac cagttggctt aaattcaccg gcacattgtt ttcaatggcg
    24361 gtgtataagc tgtcgtaaac gccagcgatt ttttcgagat agtgttccag aacctgttcg
    24421 cgatcgcgga tgttgacctt gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca
    24481 atccgctgtt tgtcttcacc atcttttgtt tcgacgtaat actcgatggt gtggctgtta
    24541 aagccatccg ccagggtgac gtaaatacga aaatgttcaa aaaggacgaa acagagttct
    24601 ttgtccggcg cgcagctcat ggcgtgatgc aggcgcgctt tcgataacgt gattccctga
    24661 accagcgaat tgcagtagat gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg
    24721 taatcggctt tctcgcttat ttcccaaata gtcattgtca ggttacccgt ttaacagaga
    24781 tgccagcttt aagcattttt tgtgccaact tttaatttat tgttattaaa gcgattttta
    24841 agctaaagat gaatttcgtc gccgtgtcga cgtgtcattt cgacatcatc gacattattc
    24901 accgcaggga taatcaacac tggcacaatt attgcttgta gctggcaata gttaatggga
    24961 ggcgatatgc acgaaataac cctctgccaa cgggcactgg aattgatcga acagcaggcc
    25021 gcaaaacacg gcgcaaaacg cgtaactggg gtctggctca aaattggcgc attttcttgt
    25081 gtcgaaacca gctctcttgc cttttgtttt gatctggttt gccgcggcag cgtggcggaa
    25141 ggttgtaaac tgcacctcga agaacaagag gccgaatgct ggtgtgaaac atgccaacag
    25201 tatgtgacgc tactgaccca gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta
    25261 cagattgtgg cagacgacgg tttacagatt cggcggatag aaatagacca ggagtgagcg
    25321 atgtgtacaa catgcggttg cggtgaaggc aacctctata ttgagggtga tgaacataac
    25381 cctcattccg cgtttcgtag cgcgccattt gccccggcgg cacgcccgaa gatgaaaatc
    25441 accggcatta aagcgcctga atttaccccc agccagactg aagaaggcga cctgcattac
    25501 ggtcatggcg aagcgggcac tcacgcgccg ggcatgagcc agcgtcggat gctggaagtc
    25561 gaaattgacg tgctggacaa aaataaccgt ctggctgaac gcaaccgcgc gcgctttgct
    25621 gcccgcaagc aactggtgct caacctggtt tccagccctg gttccggtaa aaccaccctg
    25681 ctgacggaaa ctttaatgcg cctgaaagac agcgttccgt gcgcggttat tgaaggtgac
    25741 cagcaaaccg tgaacgatgc cgcacgcatt cgcgctaccg gcaccccagc gattcaggtg
    25801 aacaccggta aaggctgcca tcttgacgca cagatgattg ccgacgccgc gccgcgcctg
    25861 ccactggacg ataacggtat tctgtttatc gaaaacgtcg gtaacctcgt ttgcccggcc
    25921 agcttcgatc tcggtgaaaa acacaaagtg gcggtgcttt ccgttaccga aggtgaagac
    25981 aaaccgctga aatatccgca catgtttgcc gccgcctcgc tgatgctgct caacaaagtt
    26041 gacttgctgc cgtatctcaa tttcgacgtt gagaagtgca tcgcctgcgc ccgtgaagtc
    26101 aatccagaaa ttgaaatcat ccttatttcc gccaccagcg gcgaagggat ggaccagtgg
    26161 ctgaactggc tggagacaca gcgatgtgca taggcgttcc cggccagatc cgcactattg
    26221 acggcaacca ggcgaaagtc gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag
    26281 tcggcagctg cgatgaaaac ggtcagccac gcgtgggcca gtgggtactg gttcacgttg
    26341 gctttgccat gagcgtaatt aatgaagccg aagcacgcga cactctcgac gccttacaaa
    26401 acatgtttga cgttgagcca gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg
    26461 ttttgttgat gaatatcgcg cgccggaaca ggtgatgcag ttaattgagc atctgcgcga
    26521 acgtgcttca catctctctt acaccgccga acgccctctg cggattatgg aagtgtgtgg
    26581 cggtcatacc cacgccattt ttaaattcgg cctcgaccag ttgctaccgg aaaacgttga
    26641 gtttatccac ggtccgggtt gcccggtgtg cgtactgcca atgggtagaa tcgacacctg
    26701 cgtggagatt gccagccatc cggaagtcat cttctgtacc tttggcgatg ccatgcgcgt
    26761 accagggaaa caggggtcgc tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt
    26821 ttactcgcca atggatgcgt taaaactggc gcaggagaat ccaacccgta aagtggtatt
    26881 cttcggctta ggttttgaaa ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa
    26941 agcccgcgat gtgcagaatt tttacttctt ctgccagcac attacgctta tcccgacgct
    27001 gcgcagtttg ctggaacagc cagataacgg tatcgacgcg ttcctcgcgc cgggccacgt
    27061 cagtatggtt atcggcactg atgcctataa ttttatcgcc agcgattttc atcgcccgct
    27121 ggtggtcgct ggtttcgaac cccttgatct actgcaaggc gtggtgatgc tggtggagca
    27181 gaaaatagcg gcccacagca aggtagagaa tcagtatcgt cgggtggtac cggatgccgg
    27241 taacctgctg gcgcaacagg cgattgccga tgtgttctgt gtcaacggcg acagtgaatg
    27301 gcgcggctta ggcgtgattg aatcttctgg cgtgcacctg acgccggatt atcaacgatt
    27361 cgatgccgaa acacatttcc gcccggcacc gcagcaggtc tgcgatgacc cgcgtgcgcg
    27421 ttgtggcgaa gtcttgacgg gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac
    27481 ctgtaatcct caaaccgcgt ttggtgcgct gatggtttcc tccgaaggag cgtgcgccgc
    27541 gtggtatcag tatcgtcagc aggagagtga agcgtgaata atatccaact cgcccacggt
    27601 agcggcggcc aggcgatgca gcaattaatc aacagcctgt ttatggaagc ctttgccaac
    27661 ccgtggctgg cagagcagga agatcaggcc cgtcttgatt tggcacagct ggtagcggaa
    27721 ggcgaccgtc tggcgttctc aaccgacagt tacgtcattg acccgctgtt ctttcctggc
    27781 ggtaatatcg gcaaactggc gatttgcggc accgccaatg acgttgcggt cagtggcgca
    27841 attccgcgct atctctcctg tggctttatc ctcgaagaag gattgccgat ggagacactg
    27901 aaagccgtag tgaccagcat ggcagaaacc gcccgcgcgg caggcattgc catcgttact
    27961 ggcgatacca aagtggtaca gcgcggcgcg gcagataaac tgtttatcaa caccgctggc
    28021 atgggcgcaa ttccggcgaa tattcactgg ggcgcacaga cgctaaccgc aggcgatgta
    28081 ttgctggtta gcggtacact cggcgaccat ggggcgacta tccttaacct gcgtgagcaa
    28141 ctagggctgg atggagaact ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg
    28201 ctgcgtgaca ttcccggcgt gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg
    28261 gtggttcatg agttcgcggc agcctgcggt tgtggtattg aactttcaga agcggcactg
    28321 ccggttaaac ctgctgtgcg cggcgtttgc gaactgctgg gactggacgc ccttaacttt
    28381 gccaacgaag gcaaactggt gatcgccgtt gaacgcaacg cagcagagca agtgctggcg
    28441 gcgttacatt cccatccatt gggaaaagat gcggcgctga ttggcgaagt agtagaacgt
    28501 aaaggtgttc gtcttgccgg tctgtatggc gtgaaacgaa ccctcgatct accgcacgcc
    28561 gaaccgcttc cgcgtatatg ctaataaaat tctaaatctc ctatagttag tcaatgacct
    28621 tttgcaccgc tttgcggtgc tttcctggaa gaacaaaatg tcatatacac cgatgagtga
    28681 tctcggacag caagggttgt tcgacatcac tcggacacta ttgcagcagc ccgatctggc
    28741 ctcgctgtgt gaggctcttt cgcaactggt aaagcgttct gcgctcgccg acaacgcggc
    28801 tattgtgttg tggcaagcgc agactcaacg tgcgtcttat tacgcatcgc gtgaaaaaga
    28861 cacccccatt aaatatgaag acgaaactgt tctggcacac ggtccggtac gcagcatttt
    28921 gtcgcgccct gatacgctgc attgcagtta cgaagaattt tgtgaaacct ggccgcagct
    28981 ggccacaggt gggctatacc ccaaatttgg tcactattgc ctgatgccac tggcggcgga
    29041 agggcatatt tttggtggct gtgaatttat tcgtcatgac gatcgcccct ggagcgaaaa
    29101 agagttcaat cgtctgcaaa catttacgca gatcgtttct gtcgtcaccg aacaaatcca
    29161 gagccgcgtc gttaacaatg tcgactatga gttgttatgc cgggaacgcg ataacttccg
    29221 catcctggtc gccatcacta acgcggtgct ttcccgcctg gatatggacg aactggtcag
    29281 cgaagtcgcc aaagaaatcc actactactt cgacattgac gatatcagca tcgtcttacg
    29341 cagccaccgt aaaaacaaac tcaacatcta ctccactcac tatcttgata aacagcatcc
    29401 cgctcacgaa cagagcgaag tcgatgaagc cggaaccctc accgaacgcg tcttcaaaag
    29461 taaagagatg ctgttgatta atctccacga gcgggacgat ttagccccct atgaacgcat
    29521 gttgttcaac acctggggca accagattca aaccttgtgc ctgttaccgc tgatgtctgg
    29581 cgacaccatg ctgggcgtgc taaaactggc gcaatgcgaa gagaaagtct ttaccactac
    29641 caatctgagc ttactgcgcc agattgccga acgtgtggca atcgctgtcg ataacgccct
    29701 cgcctatcag gaaattcatc gtctgaaaga acggctggtt gatgaaaacc tcgccctgac
    29761 cgagcagctc aacaatgttg atagtgaatt tggcgagatt attggccgca gcgaagccat
    29821 gtacagcgta cttaaacaag tggaaatggt ggcgcaaagt gacagtaccg ttctgatcct
    29881 cggtgaaact ggtacaggta aagagctgat tgcccgcgcg atccataatc tcagtgggcg
    29941 taataatcgc cgtatggtca aaatgaactg cgcggcgatg cctgccggat tgctggaaag
    30001 cgatctattt ggtcatgagc gtggggcttt taccggtgcc agcgctcagc gcattggtcg
    30061 ttttgaactg gcggataaaa gctcactatt cctcgatgaa gtgggcgata tgccgctgga
    30121 gttacagccg aagttgctgc gtgtattaca ggaacaggag tttgaacgtc tcggcagcaa
    30181 caaaatcatt cagacggacg tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat
    30241 ggtcgccgac cgagagttcc gtagcgatct ctattaccgt ctgaacgtat tcccgatcca
    30301 cctgccgcca ctacgcgagc gtccggaaga tattccgctg ctggcgaaag cctttacctt
    30361 caaaattgcc cgtcgtctgg ggcgcaatat cgacagcatt cctgccgaga cgctgcgcat
    30421 cctgagcaac atggaatggc cgggcaacgt gcgtgagctg gaaaacgtca ttgagcgcgc
    30481 ggtattgcta acacgcggca acgtgctgca gctgtcattg ccagatattg ctttaccaga
    30541 acctgaaacg ccgcctgccg caacggttgt cgcccaggag ggcgaagatg aatatcagtt
    30601 gattgttcgc gtgctgaaag aaacaaacgg cgtggttgcc gggcctaaag gcgctgcgca
    30661 acgtctgggg ctgaaacgca cgactctgct gtcacggatg aagcgactgg gaattgataa
    30721 atcggcattg atttaacaga tgtggttata ctcgatcaac attcaatgaa aggaggcagt
    30781 atggcagttt cagcaagaaa tcaattaacc ggaacagtta gcacagtagc aatgggcgct
    30841 gtgaatgatg aagtagaact gacgctggca ggtggtgcaa agctggtggc aattgtgact
    30901 cacagcagcc agcaagcgct gggtctggcg aaagggaaag aagcgattgc cctgattaaa
    30961 gccccgtggg tcacgctggc aacggaagac tgtggactga aattctctgc tcgcaaccag
    31021 tttgctggca gcgtgtcgac aatcaccgaa ggcgcagtga acgccactgt ccatatcaaa
    31081 acggatgcgg gttttgaaat tgtggcagtg gtaacaaatg aaagtcagga tgaaatgaaa
    31141 ctcaccactg gctcgcgcgt tattgcattg atcaaagcat cagccatcct gattgccacc
    31201 aaagcgtaaa cgccttattt gccggagagt atggctctcc ggcagattta tttctttgga
    31261 cgatattttt ctggcaactc cggcaccgga cgcttgtcat cgatgagatg acgcacggtt
    31321 aagatcggat gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct
    31381 tcacgctttg caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttca
    31441 ccgaacacac atttatccag ccgcttttgc gcgtaagcaa acaacgcctc gtaatgctcc
    31501 ggctctgctg acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc
    31561 agtttttcac gagagatacg cttgccggac atgctgcctc catactctta aggtgcattt
    31621 atattacaac ttaattttaa aggggactat ggctctgaag gaggaggttt cttcaaccga
    31681 cgaatttggc gatcttgttt catcgtcatc acgcaaaagc tgcaaagcag catttttcgc
    31741 ggaaccgaca tcaagaactc acctttcgcg tcttcccctg aaatgattaa ctccggtatc
    31801 atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc acactacatt taacatcagg
    31861 gagccggact taaccccatg agaacaatag aaaatttcga cgcccatacg cctatgatgc
    31921 agcagtatct caagctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggcg
    31981 acttttatga actgttttat gacgacgcaa aacgggcgtc gcaactgctg gatatttcac
    32041 tgaccaaacg cggtgcttca gcgggagagc caatcccgat ggcggggatt ccctaccatg
    32101 cggtggaaaa ctacctcgcc aaactggtga atcagggcga atcggttgct atttgtgaac
    32161 agattggcga tccggcgacc agcaaagggc cggtcgagcg caaagttgtc cgtatcgtta
    32221 cgccgggcac catcagcgat gaagcgctgt tgcaggaacg tcaggacaac ctgctggcgg
    32281 ctatctggca ggacagcaag gggttcggct acgccacgct ggatatcagc tccgggcgtt
    32341 ttcgcctgag cgaaccggcg gaccgcgaaa cgatggcggc agaactgcaa cgtacgaatc
    32401 cagcggaatt actgtatgcg gaagatttcg ccgagatgtc gctgattgaa ggtcgccgcg
    32461 gcctgcgtcg tcgcccgctg tgggagtttg aaatcgacac cgctcgccag caattgaacc
    32521 tgcaatttgg cacccgagat ctggtcggtt ttggtgtgga gaacgcacca cgcggacttt
    32581 gtgctgccgg ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata
    32641 ttcgttctat cactatggaa cgtcagcagg acagcatcat tatggatgcc gcgacgcgtc
    32701 gtaacctgga aattactcag aacctggccg gcggtgcgga aaatacgctg gcttcagtgc
    32761 tcgactgtac tgtaacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag
    32821 tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca
    32881 ccgccgagct gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcgcgtc
    32941 tggctttacg aactgctcgt ccacgcgatc tggcccgtat gcgtcacgct ttccagcaac
    33001 tgccggagct gcgcgcgcaa ctggaaggtg tcgatagcgc cccggtacaa gcactgcgtg
    33061 agaagatggg cgagtttgcc gaactgcgcg acctgctgga gcgagcaatc atcgacacgc
    33121 cgccggtgct ggtacgcgac ggtggtgtta ttgcgactgg ttataacgaa gaactggatg
    33181 aatggcgcgc gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc
    33241 gtgaacgtac tggcctggac accctgaaag tcggctttaa cgcggttcat ggttattaca
    33301 ttcagatcag ccgtggacaa agccatctgg cacccatcaa ttacatgcgt cgccagacgc
    33361 tgaaaaacgc cgagcgctac attattccag agctaaaaga gtacgaagac aaagttctca
    33421 cttcaaaagg caaagcactg gcactggaaa aacagcttta tgaagagttg ttcgacctgc
    33481 tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc
    33541 tggtgaacct ggcggaacgg gcctatacgc tgaactacac ctgcccgacc tttattgata
    33601 aaccgggtat tcgcatcacc gaaggccgcc atccggtggt tgaacaggtg ctgaatgagc
    33661 cgtttatcgc caacccgctg aacctgtcgc cgcagcgtcg catgttgatc attaccggtc
    33721 cgaatatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct
    33781 acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta
    33841 cccgcgtagg ggcagcggat gatctggcat ccgggcgttc aacctttatg gtggaaatga
    33901 ccgaaaccgc caatatttta cataacgcca ccgagtacag tctggtgctg atggatgaga
    33961 ttgggcgtgg aacgtccact tacgatggtc tgtcgctggc gtgggcgtgc gcggaaaatc
    34021 tggcgaataa gattaaagcg ttgacgttgt ttgctaccca ctatttcgag ctgacccagt
    34081 taccggagaa aatggaaggc gtcgccaacg tgcatctcga tgcactggag cacggcgaca
    34141 ccattgcctt tatgcacagc gtgcaggatg gcgcagcgag caaaagctac ggcctggcgg
    34201 ttgcagctct ggccggcgtg ccaaaagagg ttattaagcg cgcacggcaa aaactgcgtg
    34261 agctggaaag catttcgccg aacgccgccg ctacgcaagt ggatggtacg caaatgtctt
    34321 tgctgtcagt gccagaagaa acgtcgcctg cggtcgaagc actggaaaat ctcgacccgg
    34381 attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat
    34441 aacaattccc gatagtcttt tgctatcggg aatattaacg acaactg
//
LOCUS       DAZGAP010000026        34402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGAP010000026 DAZGAP010000000
VERSION     DAZGAP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.026"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..1915)
                     /gene="gss"
                     /locus_tag="ACHLAM_001082"
     CDS             complement(56..1915)
                     /gene="gss"
                     /locus_tag="ACHLAM_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO7852647.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2120..2986
                     /gene="yghU"
                     /locus_tag="ACHLAM_001083"
     CDS             2120..2986
                     /gene="yghU"
                     /locus_tag="ACHLAM_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO7852648.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3109..3357)
                     /gene="hybG"
                     /locus_tag="ACHLAM_001084"
     CDS             complement(3109..3357)
                     /gene="hybG"
                     /locus_tag="ACHLAM_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO7852649.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3370..3711)
                     /gene="hypA"
                     /locus_tag="ACHLAM_001085"
     CDS             complement(3370..3711)
                     /gene="hypA"
                     /locus_tag="ACHLAM_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7852650.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3704..4192)
                     /gene="hybE"
                     /locus_tag="ACHLAM_001086"
     CDS             complement(3704..4192)
                     /gene="hybE"
                     /locus_tag="ACHLAM_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO7852651.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4185..4679)
                     /gene="hybD"
                     /locus_tag="ACHLAM_001087"
     CDS             complement(4185..4679)
                     /gene="hybD"
                     /locus_tag="ACHLAM_001087"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO7852652.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4679..6382)
                     /gene="hybC"
                     /locus_tag="ACHLAM_001088"
     CDS             complement(4679..6382)
                     /gene="hybC"
                     /locus_tag="ACHLAM_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO7852653.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6379..7557)
                     /gene="hybB"
                     /locus_tag="ACHLAM_001089"
     CDS             complement(6379..7557)
                     /gene="hybB"
                     /locus_tag="ACHLAM_001089"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO7852654.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICTYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7547..8533)
                     /gene="hybA"
                     /locus_tag="ACHLAM_001090"
     CDS             complement(7547..8533)
                     /gene="hybA"
                     /locus_tag="ACHLAM_001090"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO7852655.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8536..9654)
                     /gene="hybO"
                     /locus_tag="ACHLAM_001091"
     CDS             complement(8536..9654)
                     /gene="hybO"
                     /locus_tag="ACHLAM_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO7852656.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9843..10130)
                     /gene="yghW"
                     /locus_tag="ACHLAM_001092"
     CDS             complement(9843..10130)
                     /gene="yghW"
                     /locus_tag="ACHLAM_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO7852657.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10249..11136)
                     /gene="yghX"
                     /locus_tag="ACHLAM_001093"
     CDS             complement(10249..11136)
                     /gene="yghX"
                     /locus_tag="ACHLAM_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO7852658.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11293..12333
                     /gene="gpr"
                     /locus_tag="ACHLAM_001094"
     CDS             11293..12333
                     /gene="gpr"
                     /locus_tag="ACHLAM_001094"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO7852659.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12373..12867)
                     /gene="yqhA"
                     /locus_tag="ACHLAM_001095"
     CDS             complement(12373..12867)
                     /gene="yqhA"
                     /locus_tag="ACHLAM_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO7852660.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13058..13942
                     /gene="yghA"
                     /locus_tag="ACHLAM_001096"
     CDS             13058..13942
                     /gene="yghA"
                     /locus_tag="ACHLAM_001096"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7852661.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(13981..14406)
                     /gene="exbD"
                     /locus_tag="ACHLAM_001097"
     CDS             complement(13981..14406)
                     /gene="exbD"
                     /locus_tag="ACHLAM_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO7852662.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14413..15147)
                     /gene="exbB"
                     /locus_tag="ACHLAM_001098"
     CDS             complement(14413..15147)
                     /gene="exbB"
                     /locus_tag="ACHLAM_001098"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7852663.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15399..16586
                     /gene="metC"
                     /locus_tag="ACHLAM_001099"
     CDS             15399..16586
                     /gene="metC"
                     /locus_tag="ACHLAM_001099"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO7852664.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16726..17385
                     /gene="yghB"
                     /locus_tag="ACHLAM_001100"
     CDS             16726..17385
                     /gene="yghB"
                     /locus_tag="ACHLAM_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO7852665.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17425..18324)
                     /gene="yqhC"
                     /locus_tag="ACHLAM_001101"
     CDS             complement(17425..18324)
                     /gene="yqhC"
                     /locus_tag="ACHLAM_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO7852666.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18518..19681
                     /gene="yqhD"
                     /locus_tag="ACHLAM_001102"
     CDS             18518..19681
                     /gene="yqhD"
                     /locus_tag="ACHLAM_001102"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO7852667.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19786..20613
                     /gene="dkgA"
                     /locus_tag="ACHLAM_001103"
     CDS             19786..20613
                     /gene="dkgA"
                     /locus_tag="ACHLAM_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO7852668.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20813..21739
                     /gene="yqhG"
                     /locus_tag="ACHLAM_001104"
     CDS             20813..21739
                     /gene="yqhG"
                     /locus_tag="ACHLAM_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO7852669.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     RIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21790..22047
                     /gene="yqhH"
                     /locus_tag="ACHLAM_001105"
     CDS             21790..22047
                     /gene="yqhH"
                     /locus_tag="ACHLAM_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO7852670.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22089..24308)
                     /gene="ygiQ"
                     /locus_tag="ACHLAM_001106"
     CDS             complement(22089..24308)
                     /gene="ygiQ"
                     /locus_tag="ACHLAM_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO7852671.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(24419..25831)
                     /gene="ftsP"
                     /locus_tag="ACHLAM_001107"
     CDS             complement(24419..25831)
                     /gene="ftsP"
                     /locus_tag="ACHLAM_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO7852672.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPTLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25906..26643)
                     /gene="plsC"
                     /locus_tag="ACHLAM_001108"
                     /gene_synonym="parF"
     CDS             complement(25906..26643)
                     /gene="plsC"
                     /locus_tag="ACHLAM_001108"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO7852673.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26877..29135)
                     /gene="parC"
                     /locus_tag="ACHLAM_001109"
     CDS             complement(26877..29135)
                     /gene="parC"
                     /locus_tag="ACHLAM_001109"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO7852674.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29273..30880)
                     /gene="ygiS"
                     /locus_tag="ACHLAM_001110"
     CDS             complement(29273..30880)
                     /gene="ygiS"
                     /locus_tag="ACHLAM_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7852675.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(30989..31471)
                     /gene="ygiV"
                     /locus_tag="ACHLAM_001111"
     CDS             complement(30989..31471)
                     /gene="ygiV"
                     /locus_tag="ACHLAM_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO7852676.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(31524..31916)
                     /gene="ygiW"
                     /locus_tag="ACHLAM_001112"
     CDS             complement(31524..31916)
                     /gene="ygiW"
                     /locus_tag="ACHLAM_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO7852677.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32068..32727
                     /gene="qseB"
                     /locus_tag="ACHLAM_001113"
     CDS             32068..32727
                     /gene="qseB"
                     /locus_tag="ACHLAM_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO7852678.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32724..34073
                     /gene="qseC"
                     /locus_tag="ACHLAM_001114"
     CDS             32724..34073
                     /gene="qseC"
                     /locus_tag="ACHLAM_001114"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO7852679.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(34119..>34402)
                     /locus_tag="ACHLAM_001115"
     CDS             complement(34119..>34402)
                     /locus_tag="ACHLAM_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO7852680.1"
                     /translation="LTYWLIDAFFAFNKYDWMLESGDDICSIPSVSGEDRILQAMIAA
                     FFLLTPLIIIILRKLFVREMFEFWLYVISLVICLVCGWWLFWGRFIFCY"
BASE COUNT         8427 a         9214 c         8692 g         8069 t
ORIGIN      
        1 ttaatccccg tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact
       61 ctttcaccac aattagcggt tcaatatcac tctctttttt gatgaccagt gattcatcac
      121 cgcgcaaaca cgtcccaccg tagttgccgc caacggtgaa ggtacatacc tgaatgtatt
      181 taccgtccac tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt
      241 taccgctggt tttgtccagc acctcttcat gatggctgac gaggtcgatg ttactgccac
      301 agcgaccggc gatcggtttc actgcataac cggttttcac cagttcatca ttcacggaga
      361 aatcggtatc cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg
      421 ctttgttgcc ggggatcact gtccacagag gttcaaagac cagcacttcc gggcgcagca
      481 atacgtcgat aagacgcact tcattttgcg gatgaccggt acggatcggc accgcagcaa
      541 actcacggtc gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc
      601 acacgcagtt aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca
      661 gttcatccag cccgcgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca
      721 taaactgcgc gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac
      781 gtgcacgact gtgtttccag gcaccagcca gttcgttaat cagcccttcc gccggattga
      841 agccgttgcc tttatagccc tgctccgccc aacgttcgag gatcaagcca gcttcggtat
      901 gacaggaggc ggagtcggcg ttgtattcgt agaccttcag gccacgttcg tccatacaga
      961 aatccatacg accggtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg
     1021 gccagaggat ttttgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt
     1081 cggttgcgtg aagatacatc aggtgcagtt cgttggtggc tttaatcagc tcctgctctg
     1141 cgctctcggt aatggtgtag taatgatacg gatcctgatt gatcacctga ccgttggcct
     1201 gcacataggc gttttgtagc ggatcttttt catccagcca tttaccgtca aactggcctt
     1261 tgttttccag gcgcgctccg ctgattttca acagctcgcc tgcaatttcc ggctgcggta
     1321 agctgtattc agtatcttcc gtctggatca tccagccgag aatggtggtg tcatcaaaag
     1381 tgtctttaag ggtatagcag ccgttttcga ccaccatctc cagctcgcgc gtccactgtt
     1441 gcccttgcgg taacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat
     1501 gcaattgggt aatgatggcg acatgaccag tgtctttaaa ttcaccgcct ttatcccaga
     1561 taagaagcgc acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca
     1621 ggatgttgtc attaaccact tcacgcagga agcgcagcga gaaaatctcc cacgccatac
     1681 ccacgtcagt gaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc
     1741 attgccattt gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt
     1801 cgtattcctg cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg
     1861 cgtagcccaa taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt
     1921 tacctttaac aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca
     1981 gcgacattaa ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa
     2041 agggttaaaa aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc
     2101 actcagaagg caactcacta tgacagacaa cacttatcag cccgcgaaag tctggacgtg
     2161 ggacaaatcc gccggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca
     2221 tgaaaaaacg ctgcccgttg gcaaacaccc attgcaactt tattcgctgg gaacgccgaa
     2281 cggtcagaaa gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga
     2341 gtacgacgcc tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga
     2401 agtgaaccca aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg
     2461 cgtgtttgag tctggatcga ttctgcttta tctggcggag aaatttggct acttcctgcc
     2521 gcaggatctg gcaaagcgta ctgaaacgat gaactggctg ttctggctac agggggctgc
     2581 accgttcctc ggcggtggtt ttggtcactt ttaccattac gcgccggtga aaattgagta
     2641 cgccatcaat cgctttacca tggaagccaa gcgcctgctc gacgtgctgg ataagcaact
     2701 ggcgcagcat aagtttgttg caggcgatga gtacaccatt gcggatatgg cgatttggcc
     2761 gtggtttggc aacgtggtgt taggcggcgt gtatgatgcc gcagagtttc tggatgcggg
     2821 aagttataaa cacgtacaac gctgggcgaa agaagtgggc gaacgtccgg cagtgaagcg
     2881 tgggcgtatt gttaaccgca tcaacggacc gctgaacgag cagttgcatg aacgccatga
     2941 cgccagtgat ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtattcgt
     3001 cgcagcaagc catccaggcc ggataaggcg ttcacgccgc atccggcaat cgagcacaat
     3061 gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa gctaatcatc acgcgctggt
     3121 aatgtcgtaa tccatttggc gcagcgcgtc taatgtggct ttggcttcat cttcgtcgat
     3181 gatgctcatg gcaaatccga cgtgtaccag tacccactgg cccagcagat cggcagggtt
     3241 accttcacaa atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc
     3301 aagctggtga atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattgt
     3361 taactccggt tattcaactt caatactttt gacgattagc gaatcgccgg tatcgacacg
     3421 caaccgctcg ccatgacaga tcggacactg cgcatcgtgc tgatgaatct ccaccacctg
     3481 gctgcaatcc cagcaccagg cctgggcggg tttatagacg atatgtaaat cgcacccttg
     3541 cgccaccgtt ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca
     3601 ggagagcgca ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc
     3661 ctgccgttgg ataatttcaa cggcgctctg gcaaagcgac aactcatgca ttttcgccac
     3721 tcctgcggcc aaatagcagg gcacgacgcc ctgcgtgtgg tacatccgga ttggtgactg
     3781 gcagcgaaag gagcattcgg gcgcagtcat ccgtcagccg ttggccctct tcaatcgaca
     3841 tgctgtgcga aagcggcgac atcagcgagc aggagaggta ttgcgaaaca ccatccagtt
     3901 caccaacagt aaaggtcata gtgccatacg gcagttgcag accaattttt tcactcactt
     3961 tgcgcagcgg ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg
     4021 tgatcacaca ccccgtccac tgaccttcga acagcgtaaa gtcagaaaca tacaccggca
     4081 ttgaaggatg cagaaaagag agatcatgca tcgaacgccg ggcaatttct tcaaaagctg
     4141 cctgtacttg cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgaatcgcc
     4201 tcccgtggga tggcttccac gcctgattca cgcagcgcag ccagaacctg ctcaagcgca
     4261 ggttcaatca tcgcttcgac cgtcggcgtc aagccaatgt gcggctccag tgattccggg
     4321 atcacgccga ccagggtcag ctttttcgga aactcaccgg tgaagcgcag ggccgacaat
     4381 acgtcggcca ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct
     4441 tcatctcgca ggatcatcat cgttcccggc gcgttctttt tcgagacaat ggcatccgca
     4501 ataatcagat gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc cgttccgcca
     4561 tcgaggatct caacataatc cggcagaatg tatcgttgct ctaacgcttc aacaattcgc
     4621 acaccgatgg cttcatcggt cagcaaaata ttgccaaccc ctaagactaa aatacgcatt
     4681 acagaacctt cactgaaacc acttcgttgc cgtcggcatc cactacgtgt accgcacagg
     4741 ccatgcacgg gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcgg
     4801 caaccggtgt acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg
     4861 ggccagagtt ccaggttgat ggaacaactg cctggtagtt actgataata ccgtctttga
     4921 tcaccatcca gtgagagagc ataccgcgcg gtgcttcaag gaaaccaacg cctttgaact
     4981 cacctgtagc cggaatgttc ggcttcacaa aggtggtgtg atcgcctttg ccgatattgg
     5041 tgatcagtgc actgtattga ttttgcagga tatcctgcaa ttcgcagcag tgaacggtac
     5101 gaccaataat acggcccagc gtggagtgca gctgcgccac ttccagcgtg ttgccagtca
     5161 gtttctgata aatcgcaacg atttcattca gtttgttctg ggtagactcg cgtcctgccg
     5221 ccagtttcac cagcatgtta gccagcggac ccacttctac cgttttgccg tagaaagtcg
     5281 gtgatttcac ccaggaatat ttaccgtcgt cagaccaacc atcataagcc ggaatggtgg
     5341 taccttccca cggtgcctgc ggcgcttcgt ctttatacca ggagtgcttc gcgctctcct
     5401 gaatgccttt aatcaggtat tcatcggagt gggaagtgat cgggcgatac gaggacagat
     5461 ccgcattctc aatgtagccg cccgggaaca ggaagctgcc gtttttgctg tcggtcggga
     5521 attccggcac gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga
     5581 acgcggcgat aactgcggta tcaaccttgt aaacctgctc aacaaagtcg ctcagtttat
     5641 cgatgaaaga cttgatgtac atcaggcgct caaggttcag cacgcccaga ccgtcgaggt
     5701 tgatcgggtt agcgacccca cctactgcca ggttctgaat gtgcggcgtt ttaccgccca
     5761 gcagcgccac gacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta
     5821 ccgcaatcag gttcacttcc ggcggcagtt tcatcgccgg gtgaccccag tagccattgg
     5881 cgaaaatccc caactgaccg ctggcaacca gatctttaat cttgttctga actttggtga
     5941 actcttccgg actgttcaga tgccaggtcg aaacgccttt cagcatttca gaggctttgg
     6001 ttgggtcagc ttgcagtgca gaagtgatgt ccacccagtc cagcgccgag agctgataga
     6061 aatgaacgat atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg
     6121 cgttaaccgg aacgtcgata ttcagtgcac tttctgccgc acgaacggaa gagagcgcgt
     6181 gagtagtcgt acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc
     6241 ggtttttcac gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa
     6301 cgacgccatt ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg gttaccggat
     6361 caatagtaat tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt
     6421 taaaggagga agtatcggca gtagacgaat gagtacgatg taagtgcaaa tctcaatagc
     6481 cacaaaacca atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc
     6541 cgggttgaat gccaccagcg aataggtcag acgccaggtt gcacaaccta acagcgcgct
     6601 cagtgctgac aggaacagca tgcgggaatc attacgcagc ttcgccacac gcagaacgac
     6661 cagcgggaag agcatcagca ggacttcaat ccagaacatc gcggagtaga agtcaccggc
     6721 aaacgctaac gacagcttgt cgcgatagat aagctcgcca aagcgcagca cgatgaaaat
     6781 cgccagcaac acactgatgg tgttggtcag cttgacgaac aggctctttt catctggacc
     6841 attgccacgc agacccgcct gcaccaacga accttcaaag atgacaatcg agaagcccat
     6901 gatgaacgcc gtcagcagcg agaacagcgg caacatttca tagctctgcc acaacggatg
     6961 cactttgtag cccgccgaga tcatcagcga ccccattgaa gactggtgca tagttggcag
     7021 cagcgcaccg agcgcgataa tgaagaacat caccttgttc agacgctgta gcgacacctt
     7081 ccagcccaat cgttcaaaca atgccggagc aaactccagt gccatcacgc cgatgtagat
     7141 ggtcatacag accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta
     7201 gaagtacggc aggttccagt agcgaccaac gtcgatggtg atcgacaagc cacccagtga
     7261 gtaaccgaac agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt
     7321 aaagacgtat accgcccacg ccagcgccca gccgccacag gcaaagccgg tgccgatcaa
     7381 caggtcaaac gcgatccaca cgccccacgg gaagccgccg ttcaggtcag agacagagcc
     7441 cagaccgaac accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa
     7501 aatcatgacc ggtttactga tgattttgcc gcccagcggt tgtggatcat gactcatgat
     7561 cgtctcctcc gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg
     7621 ccagcacagc cagtggtagc atcatgcctt tatacagggt gtgttgaata ttttcggaac
     7681 gcgcaccggt agaaagatcg tccagtttcg gcaggtcgag attttcgtaa ggcacaccgg
     7741 tcagtaccag aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt
     7801 tcggcaccgt atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt
     7861 cgctgcctgg cttcagcgcc agacgttttt tcgcctcagc catcagctct tcacgcgtac
     7921 caaaaatcac cgcgcccgcc gggcacactt ctacgcagcc aggcagaccg cctttatcaa
     7981 gacgttccac acctttctgg ttgcacagtt cacacttatg cagcgcacca aatgggttgt
     8041 tgtagtcgta cttcggcacg ttgtatgggc aggcgaccat gcagtaacgg cagccagtgc
     8101 acacatcttt gtcgtaatgg acaatgccgg ttttcggatc ttttttcagc gcagagaccg
     8161 ggcacacaga gacacaattc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt
     8221 agccgttctc ctcctggtct ttgttgaccc ccgagccgct ggtccacacc tgaatgatgt
     8281 tattggtgta cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac
     8341 gttcagggaa attgatatcc tgacacttgg tgacgcaagc ctggcagcca acgcacaagg
     8401 tcgagtcgta caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat
     8461 gactgacaga cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat
     8521 tacgtctgtt cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc
     8581 cagttcacgc accgccatca cgctgacacc ggcaaccagc ccaaccacac cgccgagcaa
     8641 accaatagcg cccgcagaga cgttgccgcc ttctttagcg ttaacgtccg gtttctgtga
     8701 acgcggagtc tgattttcga cgttggcaag ctgatggatg cctttatgga agccgatacc
     8761 ttcttcgtta cagccatagc acgggtgacc aatcgctacc ggccacacgc cgccaacgtc
     8821 gcagaattgc agcgttgagc agttgccgta tgtttctggc cctttacagc cgaggtggta
     8881 caggcaccag ccttcgcggt ggccttcatc accgaactct ttggcaaaac gaccagcatc
     8941 gaagtgcggg cgacgttcgc agtgttcgtg aatcagacgg ccataggcga aggtcggacg
     9001 gtttttgtca tccagtttcg gcggcttgcc gtaagtgatg atgtgcgcaa cggtcgcgag
     9061 gaagttgtgc gggttcggcg ggcagcccgg aatattgata acggttttgc ctggcagaac
     9121 ttcttgcagg ctgactgcgc cagttgggtt aactccagcg gcggcaacac cgccccacgc
     9181 agagcaggaa ccgatagcga taatggctgc tgcgccttcc gccgctttgc ggatgtgatc
     9241 cacaatcggc tcaccggcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc
     9301 atccaccact aacacatact gccctttgta cttctcgaga gcgttatgtt tgttctcttc
     9361 gacctggtga ccgaaagcag cagaaagcac ctcgtgatac tccagagaga tagtctccag
     9421 cacgaggttt tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca
     9481 ctcctgcgcg ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcggccat
     9541 ctctgcagcg gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat
     9601 gaaatcacga cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag
     9661 ttattccgtt gcgaagacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat
     9721 cgatgcgcca tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc
     9781 gctaaaacga agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt
     9841 gatcaatttt cgaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc
     9901 tcacgaaaga aatccattac catctcttta tccagtccat agcggcgcgt cagaataccc
     9961 gcttcccagg cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct
    10021 aatacaaaac cgcgtttata atcggcacag tattttgtca cattaaccgc actgtcggca
    10081 tgcgttgctt ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacctc
    10141 attcgctata tattgccgta tatagcgata ttttaggcaa agaacgacat ttgcgcacgc
    10201 tattttttca tgcttgttta agacgtaatc ctgtaagaga taaacccatc aggagagata
    10261 tttatcgaac catttcagcg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg
    10321 gggcgtggaa tcattatgga atccgtgatt aacccccgga tatatatacg cctcatagac
    10381 cttattattg gctttcaacg ccgcctcgta agcaggccag ccctcgttga ttcgggtgtc
    10441 cagttccgcg tagtgcaaca gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc
    10501 ctgacgacca taaaacggca ccgcgcaggc cagttccggg taagcgaccg ctgccgcgtt
    10561 cgatacgcca ccgccatagc aaaatccagt aatacccact ttgcctgtcg cttgcggata
    10621 gctttgcata aactcaatcg cggcaaagaa atcattcatc agtttggttg gatcaacctg
    10681 ctgttgcagc tcacgacctt tatcatcatt acccggataa cctccaacgg aacttaagcc
    10741 gtcaggtgcc agggcgatat accccgcctt cgccactcgc cgcgtcacat cttcgatata
    10801 cggattcagc cctcgattct catgcaccac taccacggct ggcgttttgc cgctcatttt
    10861 tgcgggcttc accagataac cccgtacctc gccgtgacca tttggcgagg gatacgtgat
    10921 gtactctgca acaatttccg ggtcggtaaa ctctacctga gtcgccagcg cataatttgg
    10981 cttgagcaaa tcaaacagcg ctaatgccgt catcccgccc accgcatact tcgccgcaag
    11041 gttgaggaac tcacgtttcg agattttccc gtgagcgtaa tagtcgtagt aatccagcaa
    11101 ctcttgtggg aaatctttgg cggttaaacg cggcatcgtt gcactcctca actggtgttt
    11161 ttttaagcaa agcataagcg cgtgtttttg cccagttttt cgtcactctg tgagccagac
    11221 tacgggatac gcgctggcga atcgctaaac tagaaacatt gtttcgaaat tgaacgatgg
    11281 aaaggagagg tcatggtctg gttagcgaat cccgaacgtt acgggcagat gcagtaccgc
    11341 tattgcggaa aaagtggttt acgcctgccc gcgttatcgc tcggtttatg gcacaatttc
    11401 ggtcacgtta acgcgctgga atcacagcgt gcgatcctgc gtaaagcgtt tgatttaggc
    11461 attacgcact ttgatttagc caacaattac gggccgcctc caggaagcgc agaagagaac
    11521 tttggtcgcc tgctgcgcga ggattttgcc gcttatcgcg atgaactgat tatctctacc
    11581 aaagccggat acgatatgtg gcccggcccg tacggctctg gcggctcacg taaatacctg
    11641 ctcgccagcc tcgaccaaag cctgaagcgt atggggcttg agtatgtcga tatcttttac
    11701 tctcatcgcg tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct ggctcatgcg
    11761 gtacaaagcg gtaaggcgct gtatgtcggg atctcctctt actcgccaga gcggacgcaa
    11821 aaaatggttg agttgctgca cgagtggaaa attccgctgt taattcatca accttcgtac
    11881 aatttactga accgctgggt ggataaaagc ggcctgctgg ataccctgca aaataacggc
    11941 gtgggctgca ttgcctttac tccactggct cagggattgc tgaccggaaa atatctcaac
    12001 gctattccgc aagattcacg gatgcatcgt gaagggaata aagttcgtgg tctgacgccg
    12061 aaaatgctca ccgaagccaa cctcaacagc ctgcgcttat tgcatgaaat ggcacagcag
    12121 cgtggacaat caatggcgca aatggcgtta agctggttgc tgaaagatga gcgagtgacg
    12181 tcggtattga ttggagccag ccgcgcggag caacttgagg agaacgtgca ggcgctgaat
    12241 aatctgacat ttagcaccga ggagctggcg cagattgatc agcatatcgc cgatggagag
    12301 ctgaatctgt ggcaggcgtc ttccgataaa tgacctgtta ataacgggcg cgagaaccgc
    12361 gcccgaatca gatcagtgat tatgacgagt cagtcggtca agatagccca tcacaaatgc
    12421 agagagcaca aacgtcagat ggataatgac gtaccacatc agtttgttat cagggacatt
    12481 tttcgcatcc ataaagacgc gcagtaagtg aatggaagaa attgccacaa tcgacgctgc
    12541 tactttgttt ttcagcgacg ttgcgtccat tttccccagc cagttcagct tctctttgtt
    12601 ctcagagata tcgagttgtg agacgaaatt ctcgtaaccg gaaaacatca ccatcaccag
    12661 caaaccgcca accagcgtca tatccaccag cgacagcaac acgaggatca aatctgattc
    12721 cgccatcgag aagatattcg gcagtacgtg aataatctcc tggaagaact tcagcgccag
    12781 ggcaactaac gccagcgaaa ggccaaagta cacgggggca agcagccagc gagaagcata
    12841 cattgcattt tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa cgggcaacag
    12901 tatatcgcaa ttaagtaaac agcacgcaac agcaaatcaa catatacgtt aaacctttgt
    12961 cgcataattc tgagggttag ggatcttcct ggttccctta tcaacgcatc gagagcgtct
    13021 acccttaaag gacgttgaac ctgaagggag aaaaatgatg tctcatttaa aagacccgac
    13081 cacgcagtat tacactggtg aatatcccaa acagaaacaa ccgacgccag gcatccaggc
    13141 gaagatgaca ccggtaccgg attgcggcga gaaaacctat gttggtagcg gtcgcctgaa
    13201 agatcgtaaa gcactggtga cagggggcga ttccggaatc ggtcgcgctg ccgccattgc
    13261 ttacgcgcgt gaaggggctg acgtggcgat cagttatctt cccgtggaag aagaagacgc
    13321 tcaggatgtc aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc tgccaggcga
    13381 tttaagcgat gagaaattcg cccgttcgct ggttcacgaa gcacacaagg cgttaggcgg
    13441 gctggatatt atggcgctgg tcgccgggaa acaggttgcc attactgata ttgcagacct
    13501 caccagcgaa cagtttcaaa agacctttgc cattaacgtt ttcgcgctgt tctggctaac
    13561 ccaggaagcg atccccctgc taccgaaagg tgccagtatc atcaccactt cgtcaatcca
    13621 ggcataccag ccaagcccgc atttactgga ctatgcggct acgaaggcgg caattctgaa
    13681 ctacagccgt ggcctggcaa aacaggtcgc ggagaaaggt attcgggtga atattgtcgc
    13741 gccaggcccg atctggacag cactgcaaat ttccggcgga caaacgcagg ataagatccc
    13801 gcagtttggt cagaaaacgc cgatgaagcg tgcggggcaa ccggcggaac tggcccctgt
    13861 atatgtttat ctggcaagtc aggaggcgag ctacgtcacc gcagaagtgc atggcgtgtg
    13921 cggcggcgag catttaggct aaaaaaatgc ccggttgtga aaagcaaccg ggcatcattt
    13981 ttatttcgct ttggcagttt cttcgccgac cagacctatc ttcaggtaac ccgcctgatg
    14041 cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttatccg ctcggaagaa
    14101 gacggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca ttgtttcatc
    14161 ggtgaccgga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca gataaaccgg
    14221 tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct tcacatctac
    14281 cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca cgtcgataaa
    14341 cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt taagatgcat
    14401 tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat gcgcagcggc
    14461 gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg caacatcacc
    14521 cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa cgaccgccgg
    14581 aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac ccggcgcaac
    14641 gactgccagg ttagtagtct gcgtttgcgc gataccaata aagctgttca tgatgcccca
    14701 gaccgtacca aacagaccaa cgaacggcga aatcgcgccg atggttgcca gatagccgtt
    14761 accacggccc atttgacgac ctactgcggc gacccgacgc tccagacgga agctggtacg
    14821 ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat tctgcgcttc
    14881 attgagcaaa tgcaggctca agcttttgct accaaaatca gcggcgatat cgttggcctg
    14941 gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc ttaagacgac gcttctgatt
    15001 gaagaactcc acgctcttac tgaagaagat tgcccaggtg actacggagg ccaaaataag
    15061 cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc agacggagag
    15121 gtccgtctgc attaaattat tacccactgt gtatctccag gacgcaagtc acaaaatctg
    15181 cgcataataa tatcaaaacg acgtcgaatt gatagtcgtt ctcattacta tttgcatact
    15241 gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc agcacgccat
    15301 ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat attcatgcta
    15361 gtttagacat ccagacgtat aaaaacagga atcccgacat ggcggacaaa aagcttgata
    15421 ctcaactggt gaatgcagga cgcagcaaaa aatacacgct cggtgcggta aatagcgtga
    15481 ttcagcgcgc gtcttcgctg gtctttgaca gtgtggaagc caaaaaacac gcgacacgta
    15541 atcgcgccaa tggcgagttg ttctatggac ggcgtggaac gttaacccat ttctccttac
    15601 aacaagcgat gtgtgaactg gaaggtggcg caggctgtgt gctatttccc tgcggggcgg
    15661 cagcggttgc taattccatt cttgcttttg tcgaacaggg cgatcatgtg ttgatgacca
    15721 acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa ctgggcgtaa
    15781 cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg ctgccaaaca
    15841 ctaaaatcgt gtttctggaa tcgccaggtt ccatcaccat ggaagtccac gatgttccgg
    15901 cgattgttgc cgccgtacgc agtgtggtcc cggatgccat cattatgatc gacaacacct
    15961 gggcagccgg tgtgctgttt aaggcgctgg attttggcat cgatgtttct attcaggccg
    16021 ccaccaagta tctggttggg cattcagatg cgatgattgg cactgcggtg tgcaatgccc
    16081 gttgctggga gcagctgcga gagaatgcct atctgatggg ccagatggtc gatgccgata
    16141 ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgc caacatcatg
    16201 aaagcagtct gaaagtggct gaatggctgg cagaacatcc ccaagtagcg cgagttaacc
    16261 accctgccct gcctggcagt aaaggccacg aattctggaa acgagacttt acaggcagca
    16321 gcgggctatt ttcctttgtg cttaagaaaa aactcaatga tgaagagctg gcgaactatc
    16381 tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa tcgttgatcc
    16441 tggcaaatca accagaacat atcgccgcta ttcgcccaca aggcgagatc gattttagcg
    16501 gaaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt gccgatctgg
    16561 acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg cagacatttt
    16621 attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg attcaggagt
    16681 acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc tgttattcaa
    16741 gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggaccc tcatattgtt
    16801 agcgttgttt actttgttat gtttgccacg ctgtttttag aaaacggctt gctgcccgcc
    16861 tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc ccagggggtt
    16921 atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg ctgctggcta
    16981 agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg gctggcacag
    17041 cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg tctgctggcg
    17101 ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat ggcgggaatt
    17161 tccggtctgc caaaccgccg cttccagttt ttcaactggt taagcggatt gctgtgggtc
    17221 agcgtggtaa ccagtttcgg ctatgcctta agtatgattc cgttcgttaa acgccatgaa
    17281 gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc tggcttgttg
    17341 ggcacgctgt ttgtggtgat taaaaaaaaa tactgtaacg cctgacgatt ttccccattc
    17401 ccggtagctg taccgggaac gtatttaatt cccctgcatc gcccgcattc ttgccgcatc
    17461 ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgcg atgcgctttc
    17521 atagccgacg cgcatcgccg ccgcgctggc cttcatgccg tcgtggatga tcatcatccg
    17581 cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg tgacagactt
    17641 aaaattatgg tggaacgccg atacgctcat gttggcttct gccgccagtt gctcgacgct
    17701 caggttttcg gtgtatttat tctcaatccg tttcagcacg cgactaatca ggctgaagtg
    17761 agtctggcgg ctgaccagtg ccagtaacgc gccgccgcaa ggtccggtca gcacgtagta
    17821 cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagcggtc gctccatcac
    17881 atccagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcag agttaatccc
    17941 gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca acagttcctg
    18001 taactgcaaa atatcgacat tgagacgcaa ccctgccagc ggcacctctg acgtggcata
    18061 ggtttcgcac tcaaacggca acggcaccgt cagcaggaga tattcattag cgtcataacg
    18121 aaacacgcgt tcattgatat aaccgatttt atgtccggaa aagagaatta tgatgccagg
    18181 ctcgtacatc accggtgtgc gtgcgaaagg cgtctcgcca tacaacaaac gcacatcggg
    18241 caacagctct gacaaactat tttctttatt tttcagttta ttaactttat ccgccagcaa
    18301 gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc ggcaatttga
    18361 ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag acattggcag
    18421 aaatgagcat tgagaggtac ggcgctggcg accacaatga aaaacatcag gcagaccgtt
    18481 ctctgccctc atattgaccc agcaaaggga gcaagtaatg aacaacttta atctgcacac
    18541 cccaacccgc attctttttg gtaaaggcgc aatcgctggt ttacgcgaac aaattcctca
    18601 cgatgctcgc gtattgatta cctacggcgg cggcagcgtg aaaaaaaccg gcgttctcga
    18661 tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta ttgagccaaa
    18721 cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaaa aagtgacttt
    18781 cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg ccgcagcggc
    18841 taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta aagagattaa
    18901 aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag aatccaacgc
    18961 cggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt ctgcccatgt
    19021 tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc cgcgtcaggt
    19081 ggctaacggt gtagtggacg cttttgtaca caccgtggaa cagtatgtta ctaaaccggt
    19141 tgatgccaaa attcaggacc gtttcgcaga aggcattttg ttgacgctga tcgaagatgg
    19201 tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtta tgtgggcggc
    19261 gactcaggcg ctgaacggtt tgatcggcgc tggcgtaccg caggactggg caacgcatat
    19321 gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac tggctatcgt
    19381 cctgcctgca ctgtggaatg aaaaacgcga taccaagcgc gctaagctgc tgcaatatgc
    19441 tgaacgcgtc tggaacatca ctgaaggttc agacgatgag cgtattgacg ccgcgattgc
    19501 cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg actacggtct
    19561 ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca tgacccaact
    19621 gggcgaaaat catgacatta cgctggatgt cagccgccgt atatacgaag ccgcccgcta
    19681 agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa gttcacaaca
    19741 cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc taatccaacc
    19801 gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt ctggcaagca
    19861 agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta tcgctcgatt
    19921 gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa aaatgcctca
    19981 gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca caagcgcccc
    20041 cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga cctctactta
    20101 atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaggg catgatcgaa
    20161 ttgcaaaaag agggattaat caaaagcatt ggcgtgtgca acttccagat ccatcacctg
    20221 caacgcctga ttgatgaaac tggcgtgacg cctgttatta atcagatcga acttcacccg
    20281 ctgatgcaac aacgccagct acatgcctgg aacgcgacac acaaaatcca gaccgaatcc
    20341 tggagtccat tagcgcaagg tgggaaaggt gttttcgatc agaaagtcat tcgcgatctg
    20401 gcagataaat acggcaaaac cccggcgcag attgttatcc gctggcatct ggatagcggc
    20461 ctggtggtga tcccaaaatc ggtcacacct tcacgcattg tcgaaaactt tgatgtctgg
    20521 gatttccgtc tcgacaaaga cgaacttggc gaaattgcaa aactcgatca gggcaagcgt
    20581 ctcggccccg atcctgacca gttcggcggc taacgtgcaa attctcccgg tggcggtaat
    20641 gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcctgcc cattgttact
    20701 cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg aagctcttaa
    20761 aggaacaact catctacacc cgggataaac gcaacggaga ggtgaaaaac agatgaaaat
    20821 aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc ctccttcaca
    20881 gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg ccagtacgcc
    20941 ttattttggt gaaaccggag agcgagcgat aacttctgcg cgtattcaac aggcgcttac
    21001 cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg atccggtttg
    21061 tgattgtcag gattttggcg atctggtgtt agaaagcgta gcgataaccc aacctgacgc
    21121 cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gacgataaag aaaagaccac
    21181 gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgacgata ttgtcagcaa
    21241 tcatggcagc gtcttacaag cagttaatag cgagaatgaa aaaacgctgg ccgctatagc
    21301 ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac atattgctga
    21361 ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg tcaatgcctt
    21421 ctataaaacc accttcaaga ctaccaacaa tcccgatgaa gatatgcgaa tagaacggca
    21481 atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac gcgttgatga
    21541 aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt ttacgctgac
    21601 caatggcaac aatgaagagc aagaactggt tttacagcgg cgcgaaggta agtgggaaat
    21661 cgctgatttt atccgcccga atagcggtag cctacttaag cagattgagg caaaaaccgc
    21721 cgccagatta aagcaatgag ctgagttaaa taacaattag ccggaacaat aaataaaagg
    21781 gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac ctgcttgctc
    21841 agtggctgcg tcaatgagca aaaggtcaat cagctggcga gcaatgtgca aacattaaat
    21901 gccaaaatcg cccggcttga gcaggatatg aaagcattac gcccacaaat ctatgctgcc
    21961 aaatccgacg ctaacagagc caatacgcgt cttgatgctc aggactattt tgattgcctg
    22021 cgctgcttgc gtatgtacgc agaatgataa aaaaattccc ggcagcattc tgttaccggg
    22081 gatatttttt aacgtccaac cgccgcttta gggcgtttct tcgcaccagc attctgcagg
    22141 cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg ggtcgccatc
    22201 ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct ggcgacgagc ctcacgcatc
    22261 tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag atgctttttg
    22321 cccatcgctt ccagcgcctg gcggattaac ggccagtttg ccggatcgtg gtaacgcaac
    22381 aacgctttat gcaggcgacg ctgcttgtcg cccttcggta caaagacgtc ttcactttta
    22441 taaccaatct tcgccagcgg gtttttgccg gtgtaataca tagtggtcga gttagccagc
    22501 ggcgacgggt agaagttctg tacctggtcg aggcggaagc ggtgcttttt cagccacagc
    22561 gccagattca ccatatcttc atcgcgcgta cccgggtgcg cggagatgaa atacgggatc
    22621 agatactgtt ctttacctgc ctgtttcgag taagtatcga acagctcttt aaagcggtca
    22681 tagctgccca tgcccggctt catcatcttc gataacggcc cttcttcggt atgttccggg
    22741 gcaatcttca gataaccgcc aacgtgatgg gtcgccagct ctttgatata gcgcggatct
    22801 tctacggcga tgtcataacg cacaccagag gcaatcagga tctttttaat gcctttcaga
    22861 tcgcgcgcac ggcgatagag gttaatcgtc ggttcgtggt tagtgtccat atgcggacaa
    22921 atatccggat aaacgcacga caaacgacgg caggtttgct cagcgcgtgg cgatttgcaa
    22981 cgcaacatat acatgttggc agtaggccca ccgagatcgg aaatcacacc cgtaaaacct
    23041 ggaacagtgt cgcggatcgc ttcgatctca ttaataatcg aatcttcgga acggctctga
    23101 ataatgcgcc cttcgtgctc ggtaatagaa cagaaagagc agccgccaaa gcagccacgc
    23161 ataatgttga ccgagaaacg aatcatttcg taagccggaa ttcgggcatt accgtaagcc
    23221 ggatgcggca cgcgcttgta cggcagcgca aagacgctat ccatctcttc ggtagaaagc
    23281 ggaatagcag gcgggttgat ccatacatag cggtcgccat gtttttgcat caatgcgcgg
    23341 gcgcagcctg ggttagtttc gtggtgaaga atgcgcgaag catgggcgta cagcacttta
    23401 tcgcccttca ctttctcgaa agaaggcagc aacacgtagg ttttttccca cggtttcggg
    23461 cgcggcggct gcacggttac ggctttcgct tcctgctttt tcggcgcaac aggtttgtta
    23521 tccgcgcacg gcagatcttc gccatacgga tgcgggatcg ggtcgatttt cccaggagta
    23581 tcaagacggg tggaatccac gccgctccag cctggcaacg cctcttttac gataatcgcg
    23641 gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg atgcgccact
    23701 tccaccagcg gacgctcacc gttaccgaac atcagcatgt cggctttcga atccaccagc
    23761 acggaacggc gcacggtatc ggaccagtaa tcataatgcg cggtacggcg cagacttgcc
    23821 tcaataccgc cgaggatcac cggtacatct ttccacgcct ctttacaacg ctgggtataa
    23881 accagtgtgg cgcgatccgg tcgcttaccc gcgacgttat ccggcgtgta ggcatcgtca
    23941 tgacgtaaac ggcgatcggc ggtatagcgg ttgatcatcg agtccatgtt gccagcagta
    24001 acaccgaaaa acagattcgg tttacccaga cgcataaagt cgtctttgct gctccagtcc
    24061 ggttgggcaa tgatcccgac acgaaagccc tgcgcttcca gcatacgacc gcaaatcgcc
    24121 atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta
    24181 tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc cggtccaaaa
    24241 caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat cagggagata
    24301 gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga ttataagccg
    24361 gaacgaaaga gaaatcgaaa ggtattccat actcgccctc ctcgggcgag tatgaagatt
    24421 acggtaccgg attgaccaac agttgcccta tcgagccacg gtccgccatt tccagcgtct
    24481 gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa accagcagct
    24541 ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg aacggcatcg
    24601 cgccattcac gttacggatc tggaacatca cgccttcaat atggaacgct tgcggctcgt
    24661 ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca atacggttga
    24721 cgtcccacag ttgtccatta atacccgggt catcacccag actgatatcg cgactgcgaa
    24781 tcggcgaacc ggccatgatt tcagttggca gcaggcgcat cggaagactg tctgtgacca
    24841 gcggcagaag gccggttggg cgcagcgtta gcaccagggt agaaaccaga atgctggatg
    24901 gctcaaagaa gccgcgaata cgatcaacaa tgctcgccgc ttcaccacag gtgatcgaca
    24961 cttcatcacc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt gccagcgaaa
    25021 gttgcttcac cgatacagga gcgggcagga atccctgatc gccagaaatc acatgtaacg
    25081 ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc aacagacgca
    25141 agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta accagcagcg
    25201 tatcaccaac aaagccgccg cttcccggtt cgttatattc cggcgtacca aagttatcca
    25261 gccgtttatc ctgaataatg accggaaaat catccacacc ataatggttg gggataggca
    25321 gcgatttgct gacttcatct tccaccagcc acattccggc aaggccgtta tagacctgct
    25381 gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc tggcgaatgg
    25441 gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg cccatcagcg
    25501 ggccaggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg cgattgctgt
    25561 aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg taacgaccat
    25621 tgattcccca aaccgatgcg cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag
    25681 tcataaacag cggttgccca cggcgagatt caagcagcgt cggaacgggt agcggttgct
    25741 gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca atccccgatg
    25801 cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact ggctaaagta
    25861 ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa cttttccggc
    25921 ggcttcgcgc tctgcgactt ctttatcgag ctcagcgatt ttttgttcca ttatcgaacg
    25981 acaatgggca gccaactcgc gaacctgatc tttgccatac tgactgacgt caattggcgg
    26041 cagcatttcg acaatcacca gaccgttgtg tagccgatta agattaatct tattcgaagt
    26101 tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt gaaatgcccc
    26161 ggtcttgaac ggcagcaggc cgcgaccacg gctgcgggtt ccttccggga acatccagat
    26221 ggaaatacgg cgttttttga agtgattcac gacttccgca atagtgccgt gagctttagt
    26281 gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc cgaagaaggg
    26341 gatccacagc aagctctttt tccctaccgt cactgtcggc ggctgcacaa tgttcgatgc
    26401 cgtcaccatg tcataattgt tctggtggtt agcaatatag atagcatttc cgtagctttc
    26461 agcatccgca ggtttgcggc actcaacttt caggccgaac agcggtgcaa gacggccaaa
    26521 catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga
    26581 gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa agatatatag
    26641 cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa tcaggttagg
    26701 gcattgttaa aagccgctct ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca
    26761 acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg atgacgactt
    26821 aacgtttcat ccggcgttcc ttgcaagcgg gaggaaacag cgccctcccc ggcatattac
    26881 tcttcgctat caccgctgct ggcacggcga ggagagtcga tctcaacacg atcgatacgc
    26941 tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcgc cagcgacttt ctgtagctct
    27001 tccggacgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct ttgcggcggc
    27061 agaacgtaca gttgcgcaag accatcctcg ccacgcgcgg cttctgccga tggaatgttg
    27121 ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg gaacatcaac
    27181 atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat caccaccggc
    27241 ggcataacat gggcattttc cggtaaggtg atcaaagcct tacctgcacg gttacgcgcc
    27301 accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat cagcagtttc
    27361 tgatcgtcgc tttccatcag catatggtca acggtcgctc caggcggcaa cgttaatttg
    27421 ccggtgagcg gctcaccctg accacgcgcc gacggcagcg taatcgggtc gatggcatag
    27481 ctacgaccgg tggaatcaac aaacactacc ggttggttgc tcttaccttt caccgccgct
    27541 ttgaagctat cgcccgcttt ataattcagg cccggcgcgt caatatcatg gcctttagcg
    27601 ctgcgcaccc agcccatctg cgacagcaca atggtgacag gttcagacgg cagcatgtcg
    27661 tgctcgctca ttgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg ttcgtcaccg
    27721 taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt acgctcggaa
    27781 gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc accgcgaatc
    27841 ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc ttccgcctgg
    27901 gtttcagtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc attacgaatg
    27961 atctcaatca cttcgtcgat attgagaaac gccaccagca aaccttcgag gatatgcagg
    28021 cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc gcggcggaac
    28081 accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc atccagaccg
    28141 atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcaaa gaggtggttc
    28201 atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg ggtcgggttc
    28261 tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt gcgcatttgc
    28321 gcagcaattt gctccagtac gcgcgcacct gaaacctgat gcggcaatgc gctgataacc
    28381 accgcgccat cttctttctt ccaaaccgcg cgcatacgca ctgaaccacg tccgttctcg
    28441 tagattttac gaatctcggc gcgcgaagtg ataatttccg cttcagtcgg ataatccggc
    28501 ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat taatgcgatt
    28561 gccgcctgag ccacttcacg caggttatgc ggtggaatat cggtcgccat gccgacggca
    28621 ataccggtgg tgccgttaag caaaatgttt ggcagacggg caggcagcat tttcggctcc
    28681 tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag ctcgctcaat
    28741 agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc gaacgatttc
    28801 ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg gtaagagaac
    28861 ggctgcgcca tcaggaccat cgcttcataa caggcactat cgccgtgcgg atggtattta
    28921 cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc attcaggccc
    28981 agctcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc accaataaac
    29041 ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt ttccgtaaat
    29101 tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc aacttattcg
    29161 ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg tcgagtcaca
    29221 ggagatcagc gtgaatagcg ccgccggacg tgctccggcg gcgataaagg catcaatgtg
    29281 ccttgatata caactctttg ctgtagacat aatcctgggg attatgcagc ggaaaaccgc
    29341 caacgtaggg tttaagcagt ttgattaacg gctgatagta gatgggaatc agcggtgcct
    29401 gctggttgat gatcacttct gcctgctgat acaacgcatt acgctttgtc gcatcagtga
    29461 tctgcgtggc ctggtttagt aaggcgtcat actgcgcatt tttccagtga ccgacgtttt
    29521 cttcactatc gcttttgagc gtgttcagga agctggaggc atcattgtac gtcgcatccc
    29581 acgactgccg agacagcata aaatcaccgg ctcgtcgggc atcaagatag gttttccact
    29641 ccattgtgcg cagcgtcacc tgtgcaccca gccatttttt ccattcggaa gacaacgcta
    29701 tcgcggtctt ttcatgcaga tcgtacttgt tgtagaacag ctcaaagcgt agcggatgag
    29761 aggcgtcgta tcccgcctgt ttcagcaagg cttttgccat cgcgacacgc tcactcattg
    29821 gcttttgcag ttcatcgaac gtcgtcgcgc taaagccttt tacctctggc ggcgtcagcg
    29881 tggttgcggg cgttctcaac cccagtacct tttgcgcaat aagctgtcga tcaaccgtaa
    29941 gatatagcgc ccgacgcacc cgcacatcgt taaatggcgg tttctcaagg ttaaagttgt
    30001 aatattcgct gttcagacgc gggataattc gtagctcgcc aggcagtgat ttttcaatgg
    30061 cgggaatttg ctgcgccgga acccaggtga gatcgacctc tcccgcgcga tagcggttat
    30121 agccggtgac cgaattatcc agcgccagat actcaacctg ttgcaatacc gtatgttgcg
    30181 catcgcggta ctttggattt ttgcgtgcgg taatcttttc gttaactacc catttatcaa
    30241 gcacaaaggc accgttgtaa accatgttct ctggcttact ccagctatcg ccatgtttag
    30301 cgatgacatg atgaggaacc gggaacaacg tcggccaggc gagcatcgtc gtaaaccaag
    30361 gaactggctg ctcaagcgta acttcaagag tacgatcatc cgtcgctttg atccccagcg
    30421 atgtaacatc tgctttaccc gcaacaatag ccgcggcatt gttaatgtgc gcctgtgcca
    30481 gatagccagc aaaagggctt gccgttttcg ggtcaaccgc acgttgccag ccgaggacaa
    30541 aatcctctgc cgtcagaggc tgaccgtctg accactgcaa accgctacgc agatggaaaa
    30601 tatagcgctt gccgccgtca agtatctccc agcgttcagc ctgagcgggc tgcacctggc
    30661 cttcaccgtc catccatacc agaccttcaa acagatccag cacaatctgc gcggcagtat
    30721 tctcctctac cttttgcggg tcgagcgtgc ctgggtcgct atgattgttg taacgaaaga
    30781 cttgttgcgg ggcgagcggt gtgttggcgg gaacgtcagc agcgtagaga ggagccgcac
    30841 ttaccaggct gaccagccat aataaatttc gcgtatacat ggtattcctt accggagaaa
    30901 ttataagccc gacaacaata cgcctgtggc attgtttgct caaacttatc gcgagtgatt
    30961 tggctcacac tccggtaaag attccccatc acgccaacgg cacataaata tccgtttcca
    31021 gtcgccgctc tgtcatccct tcggcaagat tggtgtagtg aaacaggatc ggggctttgc
    31081 gcattttttc gccgcttgca ggtagccagt ggcgaatgat gccccatatc gtgtgtgaaa
    31141 tatcgtccag ctcgccaaca tggcgggcaa cagcataacg cccaccggta agttcgccat
    31201 tactcacgcc ataacgatta tcggggatcg gttcgctaac gctgccgcag atatcaaagc
    31261 gaaacgcttc tggtgcggtg gtggctgggt cgtcccaggc gacgccaaaa gtctggcttt
    31321 ggttaacagg agaaagcccc gtctctttac gccacatgat aaatttcgcc acgctgtagt
    31381 tgagcaactc agggctacag cggtgcggca acatcgccac agggattgat gggaaatcga
    31441 taatatttac atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt attcccggga
    31501 acgcgtcccg ggagcggtaa caattacgga tttactttgc ggatctgttt gacgtcaatt
    31561 tcaacagagt tccagtcttt atcgacttcc ccctgaatct caaccgtatc tttcggcgtc
    31621 accgtcacgc cgttccagcg tttatggtcg atatcaacat tgatagtacc gctggcatct
    31681 ttgaacacgt agagatcgtc agagatgcgt tcaacgatat tgccgcgtag ggttacccag
    31741 gtgtcgtcac gcagggattt tgcgctttct acagtcgtta cgctgccgtt cggcccctgg
    31801 aatcctccgg cctgactttg cgttgccgat gggccagaaa aacctccctg ctctgctgcc
    31861 ataaccggtg cactgcacag ggccattact gcgattactg ctgcgaattt tttcatgttt
    31921 attactccct ttaatgtctg tttccgagca tttaacaaga tagtccttaa caacttctta
    31981 agggaaaaaa ataaaattta atgctgtaca gagcgcgtta caacacggtt tactggcagc
    32041 aaatacggtt atcgcaggga tgaaaaaatg cgaattttac tgatagaaga tgacatgctg
    32101 attggcgacg gcatcaaaac gggccttagt aaaatgggtt ttagcgtcga ctggtttaca
    32161 caaggtcgtc agggaaaaga ggcgctatat agcgcacctt atgatgcggt gatcctggat
    32221 ttaaccttac cgggcatgga tggtcgcgat attttgcgcg aatggcgaga aaaaggtcag
    32281 cgtgagccgg tattgatcct gactgcgcgc gatgcgctgg cggaacgtgt agaagggctg
    32341 cgtctgggag ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg
    32401 ctggaagctc tgatgcgccg aaccaacggc caggccagca acgagctgcg ccacggcaac
    32461 gtcatgctag accccggcaa acgtatcgcc acgctggctg gcgacccctt aacgctgaaa
    32521 ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt actgccgcgc
    32581 aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa
    32641 gtgcatgtgc atcatctgcg acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt
    32701 attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg cgagtcaggc
    32761 tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc tttgtcgcct
    32821 ggaaacaaac aacggataac gtcgatgagt tgttcgacac ccaactgatg ctgtttgcca
    32881 aacggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc
    32941 caaataaatt aaaacacggt catgttgatg acgatgcgct gacttttgcc atctttaccc
    33001 acgacggcag aatggtcctt aacgatggcg ataacggaga agatattcct tatagctatc
    33061 agcgggaagg ttttgctgac gggcaactgg tcggtgaaga cgatccctgg cgttttgtct
    33121 ggatgacctc acctgatggc aaatatcgca tcgttgtggg tcaggaatgg gaataccgtg
    33181 aagacatggc gctggcgatt gttgccggac aattgatccc gtggctggtc gcactgccaa
    33241 ttatgttaat catcatgatg gtactactgg gtcgtgaact cgcgccgctg aacaaactgg
    33301 cgctggcact acgtatgcgt gaccctgact cggaaaaacc actaaacgcg acgggcgtac
    33361 ccagcgaagt gcgcccactg gttgagtcgc taaatcaact gttcgcccgc acacatacga
    33421 tgatggttcg tgaacgacgc tttacctccg acgcagctca cgaacttcgt agcccgttaa
    33481 cggcgctgaa agtgcaaacc gaagttgctc agctctctga cgatgatccg caggcgcgga
    33541 aaaaagcgct gcttcaatta cattccggaa tcgatcgtgc tactcgtctg gttgatcaac
    33601 tgctcacgct atcgcggctg gactcactgg ataaccttca ggacgtcgcg gagatcccgc
    33661 ttgaagatct cctgcaatcg tcggtgatgg atatttacca cacggcgcag caggcgaaca
    33721 ttgacgtgcg actgacactc aatgccaacg gcatcaaacg caccggacaa ccgctattgc
    33781 taagtttgtt ggtgcgaaat ttgctggata acgccgtgcg ctacagtcca cagggcagcg
    33841 tggtggacgt cacgctgaat gccgataatt tcatcgtgag ggataacggc cccggtgtga
    33901 caccagaggc actggcgaga attggcgaac gcttctatcg cccacccgga caaaccgcta
    33961 ccggcagtgg acttgggcta tcaattgtcc agcgaatcgc caagctgcat gacatgaatg
    34021 ttgaatttgg gaatgcggaa caaggtggat ttgaggcgaa agtaagctgg taacttctct
    34081 gtctatgaga gccgttaaaa cgactctcat agattttatt aatagcaaaa tataaaccgt
    34141 ccccaaaaaa gccaccaacc acaaaccaaa caaataacga gcgagattac atataaccaa
    34201 aactcaaaca tttctcgcac aaagagtttt cttaagataa taataataag tggcgtcaga
    34261 agaaaaaatg ctgcaatcat tgcttgcaat atacgatctt cgcctgaaac ggaaggtata
    34321 ctgcaaatat catctcccga ctcaagcatc cagtcatact tattgaaagc aaaaaaagca
    34381 tctattaacc aataagtaag ca
//
LOCUS       DAZGAP010000027        33746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGAP010000027 DAZGAP010000000
VERSION     DAZGAP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.027"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..376
                     /gene="ytcA"
                     /locus_tag="ACHLAM_001116"
     CDS             101..376
                     /gene="ytcA"
                     /locus_tag="ACHLAM_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO7852681.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            395..1426
                     /gene="mdtN"
                     /locus_tag="ACHLAM_001117"
     CDS             395..1426
                     /gene="mdtN"
                     /locus_tag="ACHLAM_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO7852682.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGIDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1426..3477
                     /gene="mdtO"
                     /locus_tag="ACHLAM_001118"
     CDS             1426..3477
                     /gene="mdtO"
                     /locus_tag="ACHLAM_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO7852683.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            3474..4940
                     /gene="mdtP"
                     /locus_tag="ACHLAM_001119"
     CDS             3474..4940
                     /gene="mdtP"
                     /locus_tag="ACHLAM_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO7852684.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPFYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTLRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            5138..7285
                     /gene="fdhF"
                     /locus_tag="ACHLAM_001120"
     CDS             5138..7285
                     /gene="fdhF"
                     /locus_tag="ACHLAM_001120"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5555..5557,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO7852685.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNKTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7379..8068
                     /gene="yjcO"
                     /locus_tag="ACHLAM_001121"
     CDS             7379..8068
                     /gene="yjcO"
                     /locus_tag="ACHLAM_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO7852686.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(8486..9799)
                     /gene="gltP"
                     /locus_tag="ACHLAM_001122"
     CDS             complement(8486..9799)
                     /gene="gltP"
                     /locus_tag="ACHLAM_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO7852687.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(10141..10737)
                     /gene="nrfG"
                     /locus_tag="ACHLAM_001123"
     CDS             complement(10141..10737)
                     /gene="nrfG"
                     /locus_tag="ACHLAM_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO7852688.1"
                     /translation="MKQSQIPVKMLTTLTILMAFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10734..11117)
                     /gene="nrfF"
                     /locus_tag="ACHLAM_001124"
     CDS             complement(10734..11117)
                     /gene="nrfF"
                     /locus_tag="ACHLAM_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO7852689.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEAEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(11110..12828)
                     /gene="nrfE"
                     /locus_tag="ACHLAM_001125"
     CDS             complement(11110..12828)
                     /gene="nrfE"
                     /locus_tag="ACHLAM_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO7852690.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(12848..13804)
                     /gene="nrfD"
                     /locus_tag="ACHLAM_001126"
     CDS             complement(12848..13804)
                     /gene="nrfD"
                     /locus_tag="ACHLAM_001126"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO7852691.1"
                     /translation="MMQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTKPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13801..14472)
                     /gene="nrfC"
                     /locus_tag="ACHLAM_001127"
     CDS             complement(13801..14472)
                     /gene="nrfC"
                     /locus_tag="ACHLAM_001127"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO7852692.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(14469..15035)
                     /gene="nrfB"
                     /locus_tag="ACHLAM_001128"
     CDS             complement(14469..15035)
                     /gene="nrfB"
                     /locus_tag="ACHLAM_001128"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO7852693.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(15080..16516)
                     /gene="nrfA"
                     /locus_tag="ACHLAM_001129"
     CDS             complement(15080..16516)
                     /gene="nrfA"
                     /locus_tag="ACHLAM_001129"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO7852694.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16909..18867
                     /gene="acs"
                     /locus_tag="ACHLAM_001130"
     CDS             16909..18867
                     /gene="acs"
                     /locus_tag="ACHLAM_001130"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO7852695.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            19249..19563
                     /gene="yjcH"
                     /locus_tag="ACHLAM_001131"
     CDS             19249..19563
                     /gene="yjcH"
                     /locus_tag="ACHLAM_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO7852696.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            19560..21209
                     /gene="actP"
                     /locus_tag="ACHLAM_001132"
     CDS             19560..21209
                     /gene="actP"
                     /locus_tag="ACHLAM_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO7852697.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(21292..22941)
                     /gene="yjcE"
                     /locus_tag="ACHLAM_001133"
     CDS             complement(21292..22941)
                     /gene="yjcE"
                     /locus_tag="ACHLAM_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO7852698.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23092..24441)
                     /gene="ghxP"
                     /locus_tag="ACHLAM_001134"
                     /gene_synonym="yjcD"
     CDS             complement(23092..24441)
                     /gene="ghxP"
                     /locus_tag="ACHLAM_001134"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO7852699.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(24987..25451)
                     /gene="soxR"
                     /locus_tag="ACHLAM_001135"
     CDS             complement(24987..25451)
                     /gene="soxR"
                     /locus_tag="ACHLAM_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO7852700.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            25537..25860
                     /gene="soxS"
                     /locus_tag="ACHLAM_001136"
     CDS             25537..25860
                     /gene="soxS"
                     /locus_tag="ACHLAM_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO7852701.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(25863..27449)
                     /gene="pdeC"
                     /locus_tag="ACHLAM_001137"
     CDS             complement(25863..27449)
                     /gene="pdeC"
                     /locus_tag="ACHLAM_001137"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO7852702.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            27879..28160
                     /gene="yjcB"
                     /locus_tag="ACHLAM_001138"
     CDS             27879..28160
                     /gene="yjcB"
                     /locus_tag="ACHLAM_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO7852703.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(28260..28796)
                     /gene="ssb1"
                     /locus_tag="ACHLAM_001139"
     CDS             complement(28260..28796)
                     /gene="ssb1"
                     /locus_tag="ACHLAM_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO7852704.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            29051..31873
                     /gene="uvrA"
                     /locus_tag="ACHLAM_001140"
     CDS             29051..31873
                     /gene="uvrA"
                     /locus_tag="ACHLAM_001140"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO7852705.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(31908..32264)
                     /gene="yjbR"
                     /locus_tag="ACHLAM_001141"
     CDS             complement(31908..32264)
                     /gene="yjbR"
                     /locus_tag="ACHLAM_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7852706.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(32268..32684)
                     /gene="yjbQ"
                     /locus_tag="ACHLAM_001142"
     CDS             complement(32268..32684)
                     /gene="yjbQ"
                     /locus_tag="ACHLAM_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO7852707.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(32795..33508)
                     /gene="aphA"
                     /locus_tag="ACHLAM_001143"
     CDS             complement(32795..33508)
                     /gene="aphA"
                     /locus_tag="ACHLAM_001143"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO7852708.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         8232 a         9140 c         8733 g         7641 t
ORIGIN      
        1 aatctatttt tattcatcga gaaaatagcc atactgacgc cttcaatgga tattgatatt
       61 cactacagat attgttcgca gcagacagga tgtatttaat atgccaacag ttctctctcg
      121 catggcgatg caactcaaaa aaacagcctg gataattccc gtcttcatgg tttcgggatg
      181 ctcattatct ccggcaatcc cggtgatcgg cgcttattat cccggctggt ttttctgcgc
      241 cattgccagc cttattttga cgctcatcac gaggcgaatt attcagcggg caaatatcaa
      301 tctggcattt gtcggaatta tttataccgc cctttttgct ctctacgcca tgctgttctg
      361 gctggcattt ttctaatcta tttgagacgc gctcatggaa agtacgccga aaaaagctcc
      421 tcgcagtaaa ttccctgctc tgttagtggt tgcgttggcg ctggttgccc ttgttttcgt
      481 tatctggcgc gtagacagtg cgccatcaac taatgacgct tacgcgtcag cagataccat
      541 tgatgtggtg ccggaagtca gcggtcgcat tgtagaactg gcggtcaccg acaaccaggc
      601 ggtgaaacag ggtgatttgc tattccgcat cgacccacgc ccatacgaag ccaatctggc
      661 gaaagccgaa gcctccctcg cggcgctgga taagcaaatt atgctcaccc agcgtagcgt
      721 tgacgcgcaa cagtttggtg ccgactcggt taatgccacg gtagaaaaag cccgtgccgc
      781 cgcgaaacag gccacagata cattacgccg caccgagcca ttactgaaag aaggttttgt
      841 ctcagcggaa gacgttgacc gtgcaagaac tgcgcagcgc gccgcagaag cggatcttaa
      901 tgccgtattg ttacaggcgc agtcagccgc cagcgccgtc agcggcatcg atgcgctggt
      961 ggcgcaacgt gcggcggtcg aagcggatat tgccctgacc aaactgcatc tggaaatggc
     1021 gaccgttcgc gcgccgtttg atggccgggt catttccctc aaaacctccg tcgggcaatt
     1081 tgcttctgcc atgcgcccta tttttaccct aatcgacact cgtcactggt acgtgatcgc
     1141 caacttccgc gaaaccgatc tgaaaaatat tcgctcaggt acacccgcaa ccattcgcct
     1201 gatgagcgac agcggcaaaa ccttcgaggg taaagtggat tcgattggct acggcgtgct
     1261 accggatgac ggcggcctgg tgctgggcgg cctgccgaaa gtgtctcgtt ctattaactg
     1321 ggtccgcgtt gcccagcgtt ttccggtcaa aatcatggtc gataaacctg acccggaaat
     1381 gttccgcatc ggcgcttcgg cagtcgctaa tcttgagccg caataatgag cgcgctcaac
     1441 tccctgccat taccggtggt caggctgctg gcgttctttc atgaagagtt aagcgagcgg
     1501 cggccaggtc gcgtgccgca gaccgtgcaa ctctgggtag gctgcctgct ggtaattctg
     1561 atctcgatga cctttgagat cccttttgtg gcgttatcgc tggcagtgct gttttacggt
     1621 attcagtcga acgcgtttta caccaaattt gtcgcgatct tgtttgtggt tgccacggtg
     1681 ctggagatcg gcagcctgtt tttgatctac aaatggtcat acggcgaacc gttgatccga
     1741 ttgatcatcg ccggaccgat cctgatgggc tgtatgtttt tgatgcgcac ccatcggttg
     1801 gggctggtct ttttcgccgt cgccatagtc gctatttacg ggcaaacctt ccccgccatg
     1861 ctcgactatc cggaagtggt cgtgcgctta accctgtggt gtatcgttgt cggcctctat
     1921 ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc ccagccgcgc tatcacgcag
     1981 atgcatcagg cgcttaatga tcggcttgat gatgccatta gccacctgac ggacagcctc
     2041 gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa actcaatgtc
     2101 ttttgcctcg cggacgatgc caactggcga actcagagcg catggtggca aagctgcgtg
     2161 gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc ttttgctgat
     2221 tctcaggcaa ttattgaatt tcggcaaaaa ttagcctcag aaatcaacaa gctgcagcat
     2281 gccattgccg aaggtcagtg ctggcaaagc gactggcgga tcactgaaag tgaagcgatg
     2341 gcggcacggg aatgtaacct ggagaatatc tgccagacgt tattgcaact gggtcagatg
     2401 gacccgaata cgccgccaac gcccgccacc aaaccgccat caatggtcgc cgacgctttt
     2461 accaatccag actatatgcg ctacgcggta aaaacgctgc tcgcctgttt gatctgttac
     2521 accttctaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac ctgcgtgatc
     2581 gtcgctaatc ccaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt tggcggggcc
     2641 ttttgcggcg cgattctggc gctgttattc acgttattgg tcatgccctg gctggacaat
     2701 attgtcgaat tgctgtttgt gctggcaccg attttcctgt tgggcgcatg gattgccacc
     2761 agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc gctcgccacg
     2821 ctcgaaaacg tttttggccc ggtgtacgac ctggtggaaa ttcgcgatcg cgctctgggt
     2881 atcatcattg gtaccgtggt gtcggcggtg atttacacct ttgtctggcc tgaaagcgaa
     2941 gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttgagtaa agtaatgcgg
     3001 atcccacgcc agcaggaagt cacagctctg cgcacttatc tgcaaattcg cataggtctg
     3061 catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggcgctgga gcgtcaactg
     3121 gacagcgaag aacgcgcttt actgattgaa cgttcgcaaa cggttattcg tcagggccgc
     3181 gatattcttc acgcctggga tgcaacctgg aactcggcgc aggcgctgga taacgcacta
     3241 cagccggaca gagcaggtca gtttgccgac gcactggaga aatacgccgc cggtctggca
     3301 accgcactca gtcgttctcc tcaaataacg cttgaagaaa cacccgcctc gcaggccatc
     3361 ctgccgacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg cttgccagac
     3421 tggacagccc cggcattaac gcccgccacg gaacaggtac aaggagccac gcaatgatca
     3481 atcgtcaact ttcacgcctg ctgttgtgca gcattctcgg cagcacgacg ctgatttccg
     3541 gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg gaacaaatca
     3601 aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag tggtggaaac
     3661 aactcaatga cccgcagttg gatgccctga tccaacggac gctaagtggt tcacacaccc
     3721 tcgccgaagc gaaactgcgg gaagaaaaag cgcagtcgca ggccgatttg ttagatgccg
     3781 gttcacaatt acaggtcgca gcgttaggga tgctcaaccg ccaacgcgtc tcggcgaacg
     3841 gctttttaag cccttatgcg atggatgcgc cagcactggg tatggacggg ccgttctata
     3901 cggaagccac agtcggtttg tttgccggac tggatctcga tttgtggggt gtgcatcgct
     3961 cagcggttgc tgccgccatt ggcgcgcata atgccgcgct ggcagaaacc gcagcagtag
     4021 agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc agctatcaga
     4081 tgctcgatct gttagaacaa acacgcgatg tgattgatta cgcggtgaaa gcgcaccaga
     4141 gtaaagtggc gcacggtctg gaagcgcaag tgcctttcca cggcgcgcgg gcacagattc
     4201 tggcggtcga taaacaaatt gttgccgtta aagggcaaat caccgaaacg cgagaatctc
     4261 tgcgtgcatt gattggcgcg ggagccagcg atatgccgga gatcaaaccg gtggcattac
     4321 cgcaagtcca gaccggcatt ccggcgacac tctcttatga gttgctcgcc agacgcccgg
     4381 atctgcaagc gatgcgctgg tatgttcagg cgtcattaga tcaggtggat tccgcgcggg
     4441 cgctgttcta tccgagcttt gatatcaaag cgtttttcgg cctggactct atccacctgg
     4501 ataccttatt caaaaaaacc agtcgccagt tcaactttat cccgggtctg aaattgccgc
     4561 tgtttgacgg tggacggttg aatgccaatc tcgaaggcac tcgcgccgcc agcaacatga
     4621 tgattgaacg ttacaaccag tcagtactga atgcggtgcg cgacgttgcc gtcaacggca
     4681 cgcgtctgca aacgctcaac gacgagcgag agatgcaggc tgaacgcgtg gaagcaacgc
     4741 gctttaccct gcgcgctgcc gaggccgcct atcagcgcgg cttaaccagc cgcttacagg
     4801 ccaccgaagc ccggttgcca gtacttgccg aagagatgtc attactgatg ctggacagcc
     4861 gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat caggcaagtc
     4921 ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc tgtcacgcgg tatttcgttt
     4981 cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa atagtgcccg
     5041 taatatcagg gaatgaccac aaataaaatg tggcataaaa gatgcatact gtagtcgaga
     5101 gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg tcacggtttg
     5161 cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca aaatcgtccg
     5221 ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg gttattatgg
     5281 ctgggatttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc ccatgatccg
     5341 tcgccagcgt ggcggcaaac tcgaacctgt ttcctgggat gaggcactga attacgttgc
     5401 cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga cgaccggctc
     5461 ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc gcgccgttat
     5521 tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg ttgcaggtct
     5581 gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg ataataccga
     5641 tttagtgttc gttttcgggt acaacccggc ggattcccac ccaatcgtgg cgaaccacgt
     5701 aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca aaattgaaac
     5761 cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg cgctgttgaa
     5821 tgcgatgggc catgtcatta ttgaagaaaa cctgtacgac aaagctttcg tcgcttcccg
     5881 tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg agtcggttga
     5941 agatatcacc ggcgtcagcg ccagtgagat tcgtcaggcg gcgcggatgt atgcccaggc
     6001 gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag ttctaccagg gcgtggaaac
     6061 cgtgcgttct ctgaccagcc tcgcgatgct gactggtaac ctcggtaagc ctcatgcggg
     6121 tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg gcgcgctgcc
     6181 ggatacgtat ccgggatacc agtacgtgaa agatccggct aaccgcgaga aattcgccaa
     6241 agcctggggc gtggaaagcc tgcctgctca taccggttat cgcatcagcg agctgccgca
     6301 ccgcgcagcg catggtgaag tgcgtgccgc gtacattatg ggcgaagatc cgctgcaaac
     6361 tgacgcggaa ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg ttatcgttca
     6421 ggacatcttt atgaccaaaa ccgcgtcggc ggcggatgtc attttaccgt caacgtcgtg
     6481 gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt tcttcaaggc
     6541 ggttgagccg aagtgggatc tgaaaacgga ctggcagatt atcagtgaaa tcgccacccg
     6601 tatgggttat ccgatgcact acaacaaaac ccaggagatc tgggatgagt tgcgtcatct
     6661 gtgcccggat ttctacggtg cgacttacga aaaaatgggc gaactgggct tcattcagtg
     6721 gccttgccgc gatacttcag ataccgatca ggggacttct tatctgttta aagagaagtt
     6781 tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc caatcgacaa
     6841 actcaccgac gagtacccga tggtactgtc aacggtgcgt gaagttggtc actactcttg
     6901 ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac ctggctacgc
     6961 acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat tggtttgggt
     7021 gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc cgaacaaagg
     7081 ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg ttaccgaaaa
     7141 cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg tcgagccgat
     7201 cgccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt tgaaaactcg
     7261 cctgcgcgaa gcggcactgg cgtaatacag tcctttctac agcctccttt cggaggctgt
     7321 ttttttatcc attcgaactc tttatactgg ttactcccta cccaatcgta ttatcaaaat
     7381 gaaaaaaatt atcgcattga tgttgttttt gacattcttt gcccacgcga acgactccga
     7441 gcctggcagc cagtatttaa aggcagcaga ggcaggggac cgacgcgcac aatattttct
     7501 tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg cacagaaagc
     7561 cgccgacagc ggtgatgctg atgcctgcgc gctgttagcg cagatcaaaa tcaccaatcc
     7621 ggtcagtctg gactatccac aagcaaaagt tcttgcagag aaagcggcgc aagcgggcag
     7681 taaagaaggt gaagtaacgc tggcgcatat tctggtaaat acgcaagcgg gtaaaccgga
     7741 ttatccaaag gcaatttcgc tgttagaaaa cgcctcggaa gatctggaga acgactctgc
     7801 cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca ttaaggccga
     7861 cgatgacaag gcaacctggt atttcaaacg cagctctgcg atttcccgaa ccggttattc
     7921 cgagtactgg gcgggaatga tgttcttaaa cggtgaagaa ggttttatcg agaagaacaa
     7981 acaaaaggcg ctgcactggt taaatctgag ctgtatggaa gggtttgata ccgggtgtga
     8041 agagtttgaa aaattaacta acggttaagg taggcctgat aagacgcggt aagcgtcgca
     8101 tcaggcattg atgtcggatg cggcgtaaac gccttatccg acctacaaaa tcacgctaaa
     8161 tcaggctatt gcgcagtctg gcgtaggcct gataagacgc ggtaagcgtc gcatcagata
     8221 ttgatgtcgg atgcggcgta aacgccttat ccgacctaca aaatcaccct aaatcaagct
     8281 attgcgcagt ctggcgtagg cctgataaga cgcggcaagc gtcgcatcag atattgatgt
     8341 cggatgcggc gtaaacgcct tatccgacct acaaaatcac cctaaatcaa gctattgcgc
     8401 agtctggcgt aggcctgata agacgcggta agcgtcgcat caggcatcca catattgccg
     8461 ggtggatatc cccggcaatc ttcaattatt gatccgcagt tttatcaaat ttgcccagca
     8521 cttcgcgctc ataagccagc gctttcttac ggtcaaattt gtgttcccac ttggcaatca
     8581 ccagcaccgc cagcgcatta cccaccacgt tcagcgcagt acgcgccatg tcgaggatac
     8641 ggtcaacacc ggcaataaac gccagacctt ccagcggaat acctacgcta cccagcgttg
     8701 ccagcaacac cacaaacgac acgcccggca cgccagcaat ccctttcgag gtcaccatca
     8761 gcgtcagcac cagaatgatt tcctgccaga tggacaggtc aatgccatac aactgcgcga
     8821 tgaaaatagc ggcaatactt tgatacagcg tcgaaccatc aaggttaaaa gagtaaccgg
     8881 tcggcaccac gaaactggtg atcgacgccg gtgctccgta ggcttccatc ttctcaataa
     8941 ttcgcggcag cacgctttca gagctggcag tggagtacgc cagaatcagc tcatctttca
     9001 gaatacgaat caggatccag acgcttaacc cgcacaggcg cgccacaatt cccagcacta
     9061 ccagcgcgaa gaacagaatg gcgaaatgca ccagcagcac cagtttcgcc agtggccaca
     9121 gagacgagaa accaaagtta gccaccgtca ccgcaatcag cgcaaacaca cccaccggcg
     9181 cataacgcat caccatgtga gtcactttaa acatggtttc agagatggag cggaaaacgg
     9241 tcaccagcgg ttcacgatgc gtcgcgggca gggatgaaag ccccagacca aacagcaccg
     9301 agaagaagat gatcggcagc atttcgcctt tcgccatcga cgccacaatg ttcgtcggca
     9361 ccagcgacaa aatcgtgccc ataatgccgt gggaactgct ttgtaccgct tccgtagtgc
     9421 tctgatattt cgagatatcg acggtcgcca gctgcgacat atccaccccg gcaccgggct
     9481 ggaagacgtt cgccagagtg atccccaaaa tgatggcgac ggtggtgatc acctcgaagt
     9541 aaataatggt tttcgcgcca atacgcccga gctgtttggc atcaccaaca cccgcgatac
     9601 ccaccaccag cgtggagatc acaatcggca caacaatcat tttgatcaga tggatgaaga
     9661 tatcacccgc cggagagagc aaattgacga ccagccagtc gcggctgtcg ctatggtagt
     9721 gcaggtagct tcccaggaga atgcccagca ccatagcaaa cagaatctgc caggccaggc
     9781 tgaattttat atttttcatc agaataactt cctcaatgga gcgccgttcc ccgttcagaa
     9841 acgcgagaac gtgacatact gccagggcat gaattgtgtt gcgttggttt gtagattttt
     9901 ttaacgctta tgatcagtat catttgcgcg gtatgttgcg caacccttta acaacagagc
     9961 ttttcgctgc attaatgcat aataacgcga gtttatggca ggattaatga ataaccattt
    10021 gttatggaaa gatttttatt tgtttaatct gcaataatac tgattggtga ttatttttgg
    10081 catatgttca ttcgattatt ttttatgcag ctcaataaca taatcttcat caccagatga
    10141 ttaatctgat ctccgctgca acaatttcgc catattaatc gactcgacca gctgtgtccg
    10201 gttgatccgg ggtgagttga gatccatcac tttttgccat agttcgatgg cctgcgcgta
    10261 attcgcctgc ataaacgcat cggaagccag cagcatcaga gcggtgattt cgttagagtc
    10321 cagcgcgagg gctttatcga tcattgcgcg tgtctgggcg gtcatatgct ggctggcctg
    10381 gtaataaagc accgtcgcca gcgccgcata cagttcagcg ttctcaccac gtagttgcaa
    10441 cgcctgacgg tacgccagca gcgaattgct gtaatcattt tgccacagat agtactcgcc
    10501 cagtaacgcc cactgttcgc tgttttgtgg attagcgcgg attttatcct gcaatgcctg
    10561 aagctgcgct tctggggttt gctggctggc aaactgatgc agcggatcgc gctgacgctg
    10621 atactccgca cgtaccgcct gccattttgg gcttaacaga taactgccga tacagagaaa
    10681 tgccatcaaa atcgtgagtg ttgtcagcat tttcaccggt atttgtgact gtttcatcgc
    10741 ttcgccctca ctcgccagag gatcagtgcc atcagcagta acaacaccac tggcagcgcc
    10801 cacaacacca gcgtctggcc cgttaacggt gggttatagc gaacaaaatc tccgtagcgt
    10861 tcggtcatcc agccgatgat ttcagcttcg ttcttcccct ccgccaccat gctgtaaacc
    10921 tgatggcgca tactgaccgc caccggcgcg ttggattcca gtaagttttg attctggcac
    10981 tgcggacaac gtaactggct ggcaatattt aacgcctgtt gctgttgttg cggattggcg
    11041 aattgccagg tgtctacgac ctgagcgcgc gcaaaacagg taaataacag cagtagcgtg
    11101 agaagtcctt tattcatcgc gcttcctccc ccgccatccg cttaacaatg cgcccgcaat
    11161 catcaacaat cctccccccc agatccagcg cacaccgctt tgtacataca aacgaaaagc
    11221 gtaacgatcc gctccggttt tctcgcccat gaccgcatac cagtcatgga tgccgttcca
    11281 gcgaattgac ggttccatca tttgctgacg gcgtgcttca taaaaacgcc gctccggcgt
    11341 cagttcacca atgcgttgct gatggtcaaa cagcgccact atcgcttttt cgctggtgta
    11401 attgcctctg gcttgcagat cgagtcgctc aaaacggaag gtgtatcctg ccagcgtcac
    11461 ctgctgaccc ggctgtaaat tcaggctgat ctcctgacga ctgacgctgg aaaccacgat
    11521 ccccgcggca aataacagca cgccagcatg agctaccagc gccggaagct gcacgcgttt
    11581 gccagagaca aacgtcgcca gcacaatcac caccagcatc aacagaccaa acggtaacgt
    11641 cgcgcggtta aaatacggcg cgccaacgga gaggcgtccc cagcccagca gaccgtaaat
    11701 catcggataa agcgttccca ccagcacgat cagtagcact gcgcaaaaca gcaacagcgt
    11761 agcgaggatt aacatttccc gcgataaccc cgaaaaacgc accgccgggc caccgtcccg
    11821 cgctcgccag ccatacagag ccagagacgc aaggctaatc agtgcaaaca ggctgaacaa
    11881 cggcacggcg cggacgttat ccagtgcgaa cgcatgaacc gaaaccagaa tgccagaacg
    11941 gacaattaag gtgcccagca gcgacagcat cagagtaact atcgccagca gcagcgacca
    12001 gtggcggaaa atcccccgct ggcgtgtcag ggacaaactg tgcagcagcg cagtggcaga
    12061 aagccagggt aataaagagg cgttttccac cggatcccag aaccaccagc cgccccagcc
    12121 cagttcgcaa taggcccacc aggaaccgag gatgatccca gccgttaatg cactccagcc
    12181 aggtaacgcc cagcgccagc aaattcgggc gcaggcacca tcaaactcgc cgcgcagtaa
    12241 actcgccagc gccacgctcg ccgccaccat caaaccgcca tagccaaggt aaagcagcgg
    12301 tggatgaaag ataagaccgg gatgttgcag catcggattg agatcgcggc cttcgattgc
    12361 tggtggaaat atccgcacga agggatcgga ccacagcact acaaacagta gcagtgcggc
    12421 gagcatgaga gataaaacgg ctagcgttag cggaaatagc ggatcggttt gctgccgata
    12481 atgccaggca aacagcgcgc tccaggcgga aagcagcagc acccaaagca gcagcgaacc
    12541 ttcatgaccg ccccacaccg ccgccagttt gagttcccac gacagcaggc tgtagctatg
    12601 ttgggcgaca taaatgaccg agaaatcgct gatgagaaaa caatacgtca gtacgccaaa
    12661 ggcgagcagc aggagcgcga actgcgccag aatgccgatg caagtgagtc gcatcatggc
    12721 aggccagcgc aaccgcactc ccgcgaaggc cgtcaacggg gtcaacacgt tgaccccgag
    12781 acttaacaac aacgccagaa aaccgacttc aggaaggaat acgtccagaa accacctctt
    12841 ttctggctta cgccaccgtc aactgtccgg cgtagagaat gaaaaagcgc agcatcaaca
    12901 cgccgaccag actggcccca cacgccgcca gcacggtagg aatgccggaa ctgcgattga
    12961 cccacggttt gagcaacatt ggcacaatca gccccagccc ggcgacacca agccagaacc
    13021 accacgtcca gaaaccgcca cctaatgccg ccaccagcgc acgcacttta ccgtcatcgc
    13081 ccagcgccag accgacaaaa aacgccacca gcaggaagat ttcaccccat accacaggga
    13141 tttccatacg gtgaacaaag tgcgcttccg tggaatgcgg atttttgcgt tggcgtatcg
    13201 ccatggcgat cagcgctacc gccgcaccgg acgagatgcc ggagaagagg aacagcaccg
    13261 gcaggatcgg gttattgagg aacggatacg atttcagcgc cgacagcaga aagccggtat
    13321 aagcccccag cagcaccgcc agcaccagca tcaatgtttc cagtccgcga tgaacgggcg
    13381 tcagcaggct cagaaccttt tgcacgatcc ccagcttcgg caaccagcgt tgttgcaggg
    13441 caagcaaatc atgttcaaag attttcgcca gccacagcac cagcaccacc atgtagagct
    13501 gaaacaacat cacccccatc gacatcaccg aggtaaaact gtagtggaac atcagcttcc
    13561 agaaggtcca cggctttgtc aggtggaaga cgaggatcaa cagaccgagg atcaccgcgc
    13621 caggcccgac aattagcgtg gtgcgcagca acgtactgtc tgcaccgccc gcctgcgggt
    13681 agaagcgacg taacagcacg gccagcgtca ccagaccggc agaaatacca atcaaaaaca
    13741 ggtagatggc aatcggccag tcccacacca gcgattcaaa atgaaatgcg gaagtctgca
    13801 tcattggctc acctccccgt atttaaacgg tacgcggtac agcttcggtt tggttcccag
    13861 cgccagcttg tagcggtaag tgggcttctg gcgcagcagt tgcgaaatct cactgttggg
    13921 atcgtccaga ttgccaaacg tcagtgcctt ggtcgggcaa gcttcaacgc acgcgggcag
    13981 cttaccggcc tgcaaattgg tcttacggca gaaatcgcat ttgtccgccg tcttcgtgac
    14041 cggatggata aagcgcacgc ggtacggaca ggcggcgatg cagtactgac aaccgacgca
    14101 gagatcagga ttaacatcga caatcccgct agcggcatca cgaaaagacg caccggtcgg
    14161 gcagacgtca acgcacggcg catgatcgca gtgctggcaa gacttacgga agaagcgata
    14221 tttcacatca ggaaattcgc cctgcggctc gctacgaata atcgtcaggc gcgagacgcc
    14281 ttccggcacc ttgttcactt cccgacaagc atccatacag gcggtacagc cgatgcataa
    14341 cgactcgtca tgcaccatgc cgtaacgcac cccattgata ttcaacgttt tcgccaccac
    14401 gcgccctgcg gtgccgctga cggctgccag cacgccgacg ccggtgagaa actggcgacg
    14461 agaccaggtc atggctgctc cttaagcaac ggaacagacg ccgggttaaa gttcggattg
    14521 gtgcgctgat cgctgtggca atcgacgcaa atcttaatcc gtcctttgtc acttaacgtc
    14581 tgcatcgtat cttgctgtgg atgcagggaa tggcagctgg cgcacgccac tttggttacg
    14641 tggacatcgt gtggccagaa cgctttttgc aactgttccg gcagatggca ggacatacag
    14701 acgctgttct gctctccaac cttgtacatc ggctcgttaa agcgcatcac atctttcacc
    14761 ccttcgcggt gttgcggtga tggctgaccg tggcagttgg tgcaggtgac cggcagtttg
    14821 ttattcgggt tgataacgga agcatgtttg ccatgcatac cttcggtatc tggcttatga
    14881 cagtccagac aggcggcatc cgggttacgc tgctgggtaa cttcgtagcg atcaccagat
    14941 gcctgcgccg caggggtagc ggtaattccg ttcaggctcc acaacaggcc cgacgccagc
    15001 accccggcag ttaataacga acgtaatacg ctcatattca ctccattgtg ttttccaccc
    15061 cttgcgaggc ggaacggggt tattggctta acagaccgtt tttacgtgcc tgctcttccc
    15121 actgcgggat caccgttttg atgaagtcct gcttctcggc cttgatttgc tccatgttca
    15181 ggccaatggc ctgctgggct ttctctttgg tcgagatatc cgggatctgg atttcatggg
    15241 taatgccttt ggtcgccagc aggcgcgcca gtttggtgcg tgcatccgcc gctttatcca
    15301 tcgccgtacc gagcatcctc agaccttctt ccggtgcgtg catatgaatg ccgtgggaag
    15361 cgatcgccaa atcccaacgc cactgggcat gacggatatc gtcctgaatc ggcttcattt
    15421 cggcttccgt tgcgcctgca tccagcgccg ctttcgcttc gaagtgagcg tgaaccagtt
    15481 gatcttcaac cttgattttc aggtcgttaa tcgactgctt acgttccgcg accacttttt
    15541 gcaaggcagc tttgtcctgg gtatggcagt tcgcacaagt ctgggcgaag ttatcaaacg
    15601 gattaccaat tttatggtcg gtgtagagtt tgccttcggc gttctgcact tttggcatat
    15661 ggcaatcgat acaggtcacg ttgtttttgc cgtgaatgcc cgctgtccag gtttcatatt
    15721 ccgggtgctg cgctttcagc attggcgttt tcgacaggga gttagtccag tcagagaagg
    15781 caattttgtc gtaatactgc tccatatttt cgactttcat gccgtcatcc cacgggaatt
    15841 taaccgcttt gtttttgccg tcgaagtaat actccacatg gcactgaccg caaaccatcg
    15901 attgctggtc gaaacgtccg gctttctcaa aaggtttacc aatggcttcc atcgcgcgag
    15961 ccgcatacgg acgggaaagg gttaactccg gtttgccttt ggcgaactct ggtgaggcgg
    16021 tgttatggca atcggcacaa cctaagttgt tgacgatttc cgggccgccg cgcgcccatt
    16081 ttccgtggaa gtagccatct tcgccgtctt tctggatcag acgcgccaca tctgggcttt
    16141 tacaactcca gcatgccatc ggtagcggac catcttcagc gtttttcggc gcaccggtac
    16201 gcagggtttc acgcacatcg gtcacagcaa aggcatgtcc acgcggcttg ttgtaatcgc
    16261 gcgagaaggg ataccccgcc cacaggatca ccagccgtgg atcttccgcc agggcgtcaa
    16321 cacgctctga ctgttccgag gtggctttcc aggagagata ttgatcggga tgttgcgggg
    16381 caaaggtttc attcttcgct tcaacagtta caggttttgc aggagctgcc gtttgttcag
    16441 cgtgaacaga agtgaaaaag aaaaaaggaa tcaataagct gaagatacgg cgtgcgttta
    16501 tttttatcct tgtcataggg gcttcatccg aattgcgcca ttgttgcaat ggcggttttt
    16561 attgtttttc acgacagtaa ccgcacctac actgtcatga cattgctcgc ccctatgtgt
    16621 aacaaataac cacactgtga atgttgtctt taatcaattg taagtgcatg taaaatacca
    16681 ctttagagtt agtcagtatc ttcctctttt tcaacagcat gcataactgc atgttcctca
    16741 aagaattaat caacttttgt tgctgacctt caaaaattac cctgccgttt atttgcacaa
    16801 ttctactttt gcgtgatctg tcgcccaaat actaaacaaa actgccaata cccctacatt
    16861 taacgcttat gccacatatt attaacatcc tacaaggaga acaaaagcat gagtcaaatt
    16921 cacaaacaca ccattcctgc caacatcgca gaccgttgcc tgataaaccc tcagcagtac
    16981 gaggcgatgt atcaacaatc tattaacgca cctgatacct tctggggcga acagggaaaa
    17041 attctcgatt ggatcaaacc ttaccagaag gtgaaaaaca cctcctttgc ccccggtaat
    17101 gtgtccatta aatggtacga ggacggcact ctgaatctgg cggcaaactg ccttgaccgc
    17161 catctgcaag aaaacggcga tcgtaccgcc atcatctggg aaggcgacga cgccagccag
    17221 agcaaacata tcagctataa agagctgcac cgcgacgtct gccgcttcgc caataccctg
    17281 ctcgagctgg gcattaaaaa aggtgatgtg gtggcgattt atatgccgat ggtgccggaa
    17341 gccgcggtcg cgatgctggc ctgcgcccgc attggcgcgg tgcattcggt aattttcggc
    17401 ggcttctcgc cggaagccgt tgccggacgc attatcgatt ctaactcacg actggtgatc
    17461 acttccgacg aaggcgtgcg tgccgggcgt agtattccgc tgaagaaaaa cgttgatgac
    17521 gcgctgaaaa acccgaacgt caccagcatt gaacatgtgg tggtactgaa gcgtactggc
    17581 gggaaaattg actggcagga agggcgcgac ctgtggtggc acgacctggt tgagcaagcc
    17641 agcgatcagc accaggcgga agagatgaac gccgaagatc cgctgtttat tctctacacc
    17701 tccggttcta ccgggaagcc gaaaggcgtg ctgcacacta ccggcggtta tctggtgtac
    17761 gcggcgctga cctttaaata tgtctttgat tatcatccgg gtgatatcta ctggtgcacc
    17821 gccgatgtgg gctgggttac cggacacagt tatttgctgt acggcccgct ggcctgcggc
    17881 gcgaccacgc tgatgtttga aggcgtaccg aactggccga cgcctgcccg tatggcgcag
    17941 gtggtggaca agcatcaggt caatattctc tataccgcgc ccacggcgat ccgcgcgctg
    18001 atggcggaag gcgataaagc gatcgaaggc actgaccgtt cgtcgctgcg cattctcggt
    18061 tccgtaggcg agccaatcaa cccggaagcg tgggagtggt actggaaaaa aatcggcaac
    18121 gagaaatgcc cggtggtcga tacctggtgg cagaccgaaa ccggcggttt catgatcacc
    18181 ccgctgcctg gcgctaccga gctgaaagcc ggttcggcaa cacgtccgtt cttcggcgtg
    18241 caaccggcgc tggtcgataa cgaaggtaac ccgctggaag gggccaccga aggcagtctg
    18301 gtgatcaccg actcctggcc gggtcaggcg cgtacgctgt ttggcgatca cgaacgtttt
    18361 gagcagacct acttctccac cttcaaaaat atgtatttca gcggcgacgg cgcgcgtcgc
    18421 gatgaagatg gctattactg gatcaccggg cgcgtggacg acgtgctgaa cgtctcgggt
    18481 caccgtctgg gaacggcgga gattgagtcg gcgctggtgg cgcatccgaa aatcgccgaa
    18541 gccgctgtcg tcggtattcc gcacaatatt aaaggtcagg cgatctacgc ctacgtcacg
    18601 cttaatcatg gggaggaacc gtcaccagaa ctgtacgcag aagtccgcaa ctgggtgcgt
    18661 aaagagattg gcccgctggc gacgccagac gtgctgcact ggaccgactc cctgcctaaa
    18721 acccgctccg gcaaaattat gcgccgtatt ctgcgcaaaa ttgcggcggg cgataccagc
    18781 aacctgggcg atacctcgac gcttgccgat cctggcgtag tcgagaagct gcttgaagag
    18841 aagcaggcta tcgcgatgcc atcgtaaccc acaattgccc gatgcgagtc ggtaacggtt
    18901 tgtaggcctg ataagacgcg acagcgtcgc atcaggcgct ggttgccgga tgcggcgtga
    18961 acgccttatc cggcctacat tcggcaacgg ctgtaggcct gataagacgc gacagcgacg
    19021 catcaggcgt tgattgccgg atgcggcgta aacgccttat ccggcctaca ttcggcaacg
    19081 gctgtaggcc tgataagacg cgacagcgtc gcatcaggcg ttgattgccg gatgcggcgt
    19141 gaatgcctta tccggcctac attcggcaag ggttacccaa gcgttaacct tctcccataa
    19201 gggagcggga attaaaacaa tccctacatt acctctggag aatctgtgat gaatggtacg
    19261 atttatcagc ggatagaaga caatgcgcat ttcagggagt tagtcgaaaa acggcaacgg
    19321 tttgccacca tcctgtcgat tattatgctg gcagtttata tcggctttat tttactgatc
    19381 gccttcgcgc ccggctggct gggcaccccg ctgaatccga acaccagcgt cacacgcggt
    19441 attccgattg gtgttggagt aattgtgatc tcctttgttc tcactggtat ctacatctgg
    19501 cgggcgaacg gcgaattcga ccgtcttaat aatgaagtcc tgcatgaggt acaagcatca
    19561 tgaaaagagt tctgacggcg cttgccgcca cactcccttt cgcagctaac gccgcggatg
    19621 ctattagcgg ggccgtagag cgccagccaa caaactggca ggcgattatt atgttcctga
    19681 ttttcgtcgt gtttacgctc ggcattacct actgggcgtc aaaacgcgta cgttcgcgta
    19741 atgactacta caccgcaggc ggcaatatca ccggttttca gaatgggctg gcgattgccg
    19801 gggactatat gtccgccgcc tcattcttgg ggatctccgc gctggtgttt acctccggct
    19861 atgacggctt aatttactcg ctgggcttcc tggtaggctg gccaatcatt ctgttcctga
    19921 ttgccgaacg tctgcgtaac ctgggccgct acacctttgc cgatgtagcc tcttaccgcc
    19981 tgaaacaagg gccgattcgt attctttcgg cctgtggttc tctggtggtg gtggcgcttt
    20041 accttatcgc ccagatggtg ggcgcaggta aactcatcga gctgctgttt ggccttaact
    20101 atcacattgc ggtggtgctg gtcggcgtgc tgatgatgat gtacgtcctg ttcggcggca
    20161 tgctggcgac cacctgggta cagattatca aagccgttct gttgctgttc ggtgccagct
    20221 ttatggcctt tatggtgatg aaacacgtcg gctttagctt caacaatctg ttcagcgaag
    20281 cgatggcggt acacccgaaa ggtgtcgata tcatgaagcc aggcgggctg gtgaaagatc
    20341 cgatctcagc actgtcgctg gggctcggac tgatgtttgg tacggcgggc ttgccgcata
    20401 ttctgatgcg cttctttacg gtcagcgatg cccgcgaagc acgtaagagc gtgttctacg
    20461 ccaccggatt tatgggctac ttctacatcc tgacctttat tatcggcttc ggcgcaatca
    20521 tgctggttgg tgcgaatccg gaatataaag atgctgcggg ccatctgatt ggtggtaaca
    20581 acatggcggc agttcacctg gccaatgcgg tgggcggcaa cctgttcctc ggctttattt
    20641 cagcggttgc tttcgccact atcctcgcgg tggtagcggg gctgacgctg gcgggcgcat
    20701 cggcggtgtc tcacgacctg tacgctaacg tcttcaaaaa aggcgcgacc gaacgtgaag
    20761 agctgcgggt atcgaaaatc accgttctga ttttgggtgt gattgccatt atcctcggcg
    20821 tgctgtttga gaatcagaac atcgccttta tggtggggct ggcgtttgcc atcgcggcga
    20881 gctgtaactt cccgatcatt ctgctttcca tgtactggtc gaaactgact acccgtggcg
    20941 caatgctggg cggctggtta ggactgatta ctgcggtggt gctgatgatc ctcggtccga
    21001 cgatttgggt acagatcctc ggtcacgaaa aagccatctt cccgtatgaa tacccggcac
    21061 tgttctctat cagcgtggca ttcctcggta tctggttctt ctcggcaacg gataattcag
    21121 cggaaggtgc gcgcgagcgt gaactgttcc gtgcgcagtt tatccgctcc cagactggct
    21181 ttggcgttga gcaaggccgc gcgcattaat ctccactagc ctctggtcac ctgaccaacg
    21241 gtaaaaaggt gcgatgtact gcaccttttt accgattaca ccgccaaagc gttattggtt
    21301 ctcttcaatc agcaacgctt caagcaaatc gagatcgtgc agtaattttt gcagcgtttc
    21361 gttactgatc tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag aacgcaatgc
    21421 cgccagacgg aagcgacgct caaggttctc ttcctgcacg gaactttcaa cgtcgttacg
    21481 tccatcggcg cgacgacgca ggttaccaat gacgcgagaa ctaacctctg tgagcagctg
    21541 gttatcgata ttctcttcgg tatccgccgc cagacgctcc tccattttct ggatcgccac
    21601 aatcgccact tctgccgttg ccgctcgcgc aatacgttcc tctttcaatt gttgcgaatg
    21661 gtccgcgact tcaatgtgtt gtagcagaat aggcaacatc accacgccga caaacagcga
    21721 gaagagaatg acaccagccg ccaggaacac cagctcatag cgcgccggga agacgttacc
    21781 atccggcaag agcagcggga ttgagagcac accagccaga gtgatcgccc cacgcacccc
    21841 ggcgaaagac gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca tcggcttctt
    21901 cttcaggaag cggttgctga actttttcat cgtccacagc cagccgaaac ggaccagcat
    21961 cagcgccgca taaatcagaa tgatatcggt aaacagcatc cagatttcga cgttagggtc
    22021 gatttctgcc gccatcagcg acgtctccag aatacccggc agctgcagac ctaacagcag
    22081 gaataccatg ccgttaaaga caaattccag catcgcccag gtactgtttg cgcgcaggcg
    22141 cattgccagc ggcgcacggc gcatcacacc ggaacgggtg atggtcatcc cggcggcaac
    22201 cgcagcgagg ataccggaaa cgccaatatg ttcggcaatc agataagaag cgaatggcag
    22261 caggaacagc aggacgatct gcgtcgcggg ttcatcaccg ccccagcggc tgaggaatcg
    22321 cagcgagcga ccgtacagcc agctcaccac aaaaccggcg agaataccgc caatggccac
    22381 tttcataaat tcgaccgtcg caccaccgac ggtgaagatc atcgtcccca tcgccactgc
    22441 cacggcaaac ttcaacgaca ccagaccgga ggcgtcattc atcaacgctt cgccctgcaa
    22501 aatgcccatg atttttttcg ggatgcgccc ttcgccaaca atccccgaga gcgccacagc
    22561 atcggtcgga gaaagcaccg ccgccagcgc aaaggcgggg atcagcggaa tgcccggcac
    22621 cacccagtaa ataagaaagc caatgccgac cacggtgacc accaccagcg ccagcgcgag
    22681 gccgaaaatc tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc agccatcagc
    22741 gaacagcaac ggcgggataa acaagactaa aaagagttca ggatcaaact ccacatgcaa
    22801 accaaacgtc ggccacgcca gtagcgcacc gatggcgatt tgcataagcg gaagcgggat
    22861 ctgaaagggc atgacacgag tgaccacccc ggacagcgag accacgaggg tcattatcag
    22921 tatggtgaag aagatttcca tgcgttccct gtttgcgatg ttttttatga tctgagaaag
    22981 gcgttatgcc tgaattctca ttttttcaga atatcgcaaa agctcacaca atgtgcggag
    23041 aaaataaaaa cgggcagcca ttgccacccg ttttcagaat catatgaaag attagatagc
    23101 ccagccaccc gcatagaagg tcaccagcgc cacagcgata acgaccgtac cgatgttcaa
    23161 cttgcgccat tcgccggaaa ccagacgacc aatcaccaga gtcgcgaagc cgatcatgat
    23221 gcctgttacg atgttacagg tcagcacgat gaataccgcc gtaaccagac ccgccatcgc
    23281 atcaacaaaa tcagcaaagt cgattttcgc cacgttactc agcatcagca ggccaacgta
    23341 catcagcgcc ggagccgttg cataccccgg aacgaggtaa gagagcggag agaggaacag
    23401 aatcagcagg aacagcacgc caacggtgat agccgtcaaa ccggttttac cgcccgccgc
    23461 cgtacccgcc gcagactcga tgtataccgc tgccggagct gcacccacca ggccagagaa
    23521 aacgctgctc atggagtcag tggtcagtgc tttcccgccg tcgatgatct gcccatcttt
    23581 atccagcagg ttcgcctggc cggcgacggc acggatagtt ccggtggcat caaatactgc
    23641 cgtcatcacc agcgccagaa cgcttggcag gactacagga ttcagcgcgc ccataatgtc
    23701 caggctgcca attagtgaat tgccgttttc atcgctcaat gaaggcatgg cgaaaacgcc
    23761 ggagaaatgg acgttaggat cgaagatcaa accgacaatt gagataccga taatggtcag
    23821 cagaatgcca ccagggactt tcagtttttc caggccgata atcactgcca gccccaccaa
    23881 cgacatcatc accggaaagg tcgtgaaatc ccccagcgcg acgggcagac catcaagtgg
    23941 gtttttaatc accagaccga caccattagc agcaatcagc agcaggaaca gaccgatacc
    24001 aatccccgtg ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc tacggatacc
    24061 cgtggcagaa atgaccgtaa acagcacacc catcaggaac acggcaccca gcgcgacagg
    24121 tacgctaata tgttgcccca gcaccagact gaatgcggta aacgccgtca gggagatggc
    24181 gcaaccaatc gccaacggca gattagccca cagacccatc acgatagaac cgagtccggc
    24241 aaccagacag gtcgcaacga aaactgccgc aggcgggaag cctgctttac ccaacatgcc
    24301 tggaacgacg ataaccgagt agaccatcgc cagaaatgtt gttaacccgg caaccacttc
    24361 ctgacggaca gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg aaccgccggt
    24421 acgcgctgat ggcgtagaca taagaaaaca tcccctgaga gtttaatttt cgtcaagatg
    24481 cgtggcggac aatcccctgc cagcgaaacg ttatcttcct gctccaggtt tgcgacaaat
    24541 tggtgcgtcg caaaaaagca aacgtttagc tccgcgcata caaaacgggt ggtaaaaaaa
    24601 gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg cttttttctc
    24661 ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat cgtcgatgcg
    24721 caagattgca gcgttatcga gccgtatacc gttcaggatt gcgataaaaa atcgtctgag
    24781 atgacaaaaa tattgcggct aaaaagtgta cttttacact ggcgaagaaa cgccggtgac
    24841 atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat gaggtgtgtt
    24901 gacgtcgggg gaaaccctcc tgtgtaccag cgggatagag agaaagacaa agaccggaaa
    24961 acaaactaaa gcgcccttgt ggcgctttag ttttgttcat cttccagcaa gcgtgcgcct
    25021 gtgccttctt ctcctaagcg gtcgcccggg ttacgcaatg ggcaatcact gcgcgaaagg
    25081 cagccacaac caatacatcc gtccagctcg tcacgcagcg ccactaaggt atgaatgcgc
    25141 cgatccaact cttctcgcca ttgggacgaa agctgtttcc actctttcgc acttaatgta
    25201 tgcccttcgg gcaacacgcc aaatgcttca cgaatggtcg ccagcggaat gccaatacgc
    25261 tgagcaattt tgatgattgc gacatatcgc aacacatcac gtttatatcg ccgctgattg
    25321 ccactattac ggatactgct aatcaaccct ttgctttcat agaaatgcag cgccgatacc
    25381 gccacaccgc tgcgttttgc cacttcgccg ggggttagca gcgctttaat gcggggtaat
    25441 ttcttttcca taaatcgctt tacctcaagt taacttgagg aattatactc cccaacagat
    25501 gaattaacga actgaacact gaaaagaggc agatttatgt cccatcagaa aattattcag
    25561 gatcttatcg catggattga cgagcatatt gaccagccgc ttaacattga tgtcgtcgca
    25621 aaaaaatcag gctattcaaa gtggtactta cagcggatgt tccgtactgt gacgcatcag
    25681 acactaggtg attacattcg ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc
    25741 accgagcgtc cgatttttga tatcgcaatg gacctgggtt atgtctctca gcagaccttc
    25801 tcccgcgttt tccgtcggca gtttgatcgc actcccagcg attatcgcca ccgcctgtag
    25861 ttttattgcc cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg cataaacacc
    25921 tgcggcggca tcgccttcgc aaagaaccat ccctgacaat agcgcacgcc gcgtttgcgc
    25981 agccagttaa cctgctcctc agtttcgacg ccttcagcga tcgtttttaa ccccagactg
    26041 tgcgccagct cgatgatatg ttccgcaatc aaatgactgg ttttatgggt ggtcagcgtc
    26101 tcaacaaacg atttgtcgat tttcaaaata tcgacattca atgatttaag gttatgcaag
    26161 ttagagtagc caataccaaa atcatcaatt gccacttcgt aaccagcctg gcggaaagcc
    26221 agaataatcg gcgtcatttt gtcgacatca agaaatgcat gttcagtcac ttcaaattta
    26281 atttgctgcg gacgcaccgc atattgctct gttttctggt tggttcgcgc tatcaaccgt
    26341 gacgtatgaa aatcggaggc cgacaggtta atagaaacat agcgatcgac atgtgttgcc
    26401 aggtacacgc ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttcgatc
    26461 atcccctcct tttctgccag cggaataaac tctgccggat tcattacttg cccctgctca
    26521 ccaggccaac gtaacaatgc ttcagctccg atacattttt ctgttttgat atcgattatt
    26581 ggctggtaat aaaggcaaag ttgatgtttt tcgagggcgc gttgcaattt gcgtttggga
    26641 gacaaatagt tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata
    26701 ccggcgggca acgcaaaaat aagatgatta taaaaatgag ttataagacg ttgatatgaa
    26761 gtcgcaacaa tggctgcaat tgggcgtttt gtcgaataaa ctgtcgcata taaatagcca
    26821 tttcgttgta cggttaaatc attcaaatga atcagcggag aaaacgttgc tgccgaggcc
    26881 tcgttgctta acgagaaaaa ggttttcgtc accgtatcat acactcccca ttgcaatgtc
    26941 ggatcatcag acatcacttc actccagaag agagggttga taaccgccac ataatttccc
    27001 cgctgcatgt aggtcatttt atagccagag aaaaaaggcg tatcacggta ataatagata
    27061 gaaacgttag gttcacgctt ataatcggcc ggtgcaatcg tatagccatt tacagacgct
    27121 atcagcgatg agcataaaaa atggttatca cgggcataga tcaattcatt aatataaaga
    27181 tagccacgaa taatattcaa cattcgcttt tgatgggctg gagtgcaaac ttgcccctga
    27241 tagcgttctg ctgcatcgct cactaaatct gcctggcgaa tgaccagctc ggatttatcc
    27301 agcgccagct gagcaaaagt ttggagctga ctattcactt ctgattttgc ccaaaggaag
    27361 gcaatccata gcgacagaat gatgggaaag agaactaaaa agataatgcc cggcaacgcc
    27421 agtaattggt gtcgtgcacg atgactcatt gctatcaatt tcctgcctgt ttatacccgg
    27481 ccattgcaag gtgaaaaccc tgggcaccga cgtctttaga taaatcttac ggtataaata
    27541 gcacttttgc ctgatttgaa attgatacct ttttaatggt cacctggata aaaataattc
    27601 attgatattc aaaactaatt ttctacagga atgcagaggc ggcgggaaga tatgccctta
    27661 atgccactga aaaaccacat aatctgtaca gcaataaaag tcacggccta atctggctaa
    27721 caaacaatca attgtgatat agttcacaaa attaatgaaa caaacagagt gtttcatttt
    27781 tgtgtcctgc aagccccgcg atagccagag tgtaccacgc ctcccgtgaa caacgccgcg
    27841 ctgtccaggg catcggctct tttcagagga attgtttgat ggctaccctc accactggcg
    27901 tggttctact tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc agcacgctca
    27961 acatccgttt tcgtcggtct gattatgtcg ggcttgcagt gatcagcagc ggtctgggcg
    28021 tggtttctgc ctgctggttc gcaatggggt tgcttggcat cacaatggcg gatatcaccg
    28081 ccatctggca caacatcgag tcggtgatga ttgaagagat gaatcagaca ccgccacaat
    28141 ggccaatgat tttaacttga tatgtagaag cctccataat ggaggcttct ttttttatgg
    28201 ctggcagatg ctttaatcat ccaccttaaa acaatataac ctattgtttt aatgacaaat
    28261 cagaacggaa tatcgtcatc aaaatccatc ggcggctcgt tagacggtgc tgccggagcg
    28321 gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccgccctg cggctgctga
    28381 ggctgacccc aaccgccctg cggctgacca ccaccgatat tgccacctgc aggagcgcca
    28441 ccaccctgac gaccacccag catctgcatg gtgccgccaa cgttcaccac gacttctgtg
    28501 gtgtagcgat cctgaccgga ttgatcggtc catttgcggg tacgcagctg accttcgata
    28561 taaacctgag aacctttacg cagatattcg ctggctactt ctgccagttt gccgaacagc
    28621 acaacgcggt gccactcagt ctgctctttc atctcgccgg tcgctttgtc acgccaggat
    28681 tcggaagtag ccagcgtaat gttggcaaca gcgccaccat ttggcatgta gcgtacttcc
    28741 gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct ggccatgttc
    28801 gtgtctcctg aaaaaaaatc gttctgaata agtgtaaacg cgcgattgta ccattaccaa
    28861 tagcgctttt actatgttgt gacctcggtt ccgggaaaca aacctggcca gacattgtta
    28921 cacaacactc cgggtaatgc attccaatac tgtatattca ttcaggtcaa tttgtgtcat
    28981 aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg
    29041 ggaaaggtga atggataaga tcgaagttcg gggcgcccgc acccataatc tcaaaaacat
    29101 caacctcgtt atcccccgcg acaagctcat tgtcgtgacc gggctttcgg gttctggcaa
    29161 atcctcgctc gctttcgaca ccttatatgc cgaagggcag cgccgttacg ttgaatccct
    29221 ttccgcctac gcgcggcagt ttctgtcact gatggagaag ccggacgtcg accatattga
    29281 ggggctttct cctgccatct caattgagca gaaatcgacg tctcataacc cgcgctctac
    29341 ggtggggaca atcaccgaaa tccacgacta tttgcgtctg ctgtacgctc gcgttggtga
    29401 gccgcgctgc ccggaccacg atgttccgct ggcggcgcaa accgtcagcc agatggtgga
    29461 taacgtgctt tcgcagccgg aaggcaagcg tctgatgctg ctcgcgccaa tcattaaaga
    29521 gcgcaaaggc gaacacacca aaacgctgga aaatctggca agccagggtt acatccgtgc
    29581 tcgtattgat ggcgaagtct gcgatctttc cgatccgccg aaactggaac tgcaaaagaa
    29641 acataccatt gaagtggtgg ttgatcgctt caaggtgcgt gacgatctca cccaacgtct
    29701 tgccgagtcg tttgaaaccg cgctggagct ttccggtggt accgcggtag ttgccgatat
    29761 ggacgatcca aaagcggaag agctgctatt ttccgccaac ttcgcctgcc caatttgcgg
    29821 ctacagtatg cgtgaactgg aaccacgcct gttttcgttt aacaacccgg cgggtgcctg
    29881 cccgacctgt gacggccttg gcgtacagca atatttcgat cctgaccgcg tgatccagaa
    29941 tccggaactg tcgctggcag gtggtgcgat ccgaggctgg gatcgccgca acttctatta
    30001 cttccagatg ctgaaatcgc tggcagatca ctataagttc gacgtcgaag cgccgtgggg
    30061 cagcctgagc gcgaatgtgc ataaagtggt gttgtacggt tctggcaaag aaaatattga
    30121 attcaaatac atgaacgatc gtggcgatac ctccattcgt cgtcatccgt tcgaaggcgt
    30181 gctgcacaat atggagcgcc gctataaaga gacggaatcc agtgcggtac gtgaagaatt
    30241 agccaagttt atcagcaatc gtccgtgcgc cagctgcgaa gggacgcgtc tgcgtcggga
    30301 agcgcgccac gtttatgtcg agaatacgcc gctacctgct atctccgaca tgagcatcgg
    30361 tcatgcgatg gaattcttca acaatctcaa actcgcaggt cagcgggcga agattgcaga
    30421 aaaaatcctt aaagagatcg gcgatcgtct gaaattcctc gttaacgtcg gcctgaatta
    30481 cctgacactt tcccgctcgg cagagacact ttctggcggt gaagcccagc gtatccgtct
    30541 ggcgagccag attggtgcgg gcctggttgg cgttatgtac gtgctggacg agccgtctat
    30601 cggcctgcac cagcgcgata acgagcgcct gctgggtacg cttatccatc tgcgcgatct
    30661 cggtaatacc gtgattgtgg tggagcacga cgaagacgcg attcgcgccg ctgatcatgt
    30721 gatcgatatc ggtccgggtg cgggtgtaca cggcggtgaa gtcgtcgcgg aaggtccgct
    30781 ggaagcaata atggcggtgc cagagtcgtt gaccgggcag tacatgagcg gtaaacgcaa
    30841 gattgaagtg ccgaagaagc gcgttccggc aaatccggaa aaagtgctga aactgacagg
    30901 cgcacgcggc aacaacctga aagacgtgac gctgacgctg ccggtgggtc tgtttacttg
    30961 catcacaggg gtttcaggtt ccggtaaatc gacactgatt aacgacacac tgttcccgat
    31021 tgcccaacgc cagttgaatg gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca
    31081 gggactggaa catttcgaca aagtgatcga tatcgaccaa agcccaattg gtcgtacccc
    31141 acgttccaat ccggcaacct ataccggcgt gtttacacct gtacgcgaac tgttcgcagg
    31201 cgtaccagaa tcccgtgcgc gtggttatac gccagggcgt ttcagcttta acgtccgtgg
    31261 cggacgctgc gaggcctgtc agggcgacgg cgtgatcaaa gtggagatgc acttcctgcc
    31321 ggacatttac gtaccgtgcg accagtgcaa aggcaaacgc tataaccgtg aaacgctgga
    31381 gattaagtac aaaggcaaaa ccatccacga agtgctggat atgaccatcg aagaggcgcg
    31441 tgagttcttt gatgcggtgc cagctctggc acgtaagctg caaacgctga tggatgttgg
    31501 cctgacgtac attcgcctcg ggcagtccgc aaccacactt tctggtggtg aagcccagcg
    31561 cgtgaagctg gcgcgtgagc tgtcaaaacg cggcaccggg cagacgctgt atattctcga
    31621 cgagccgacc accggtctgc acttcgccga tattcagcaa ctgctcgacg tactgcataa
    31681 actgcgcgat cagggcaata ccattgtggt aattgagcac aatctcgacg tgatcaaaac
    31741 cgctgactgg attgtcgacc tgggaccgga aggcggcagt ggcggcggcg aaatcctcgt
    31801 ctccggtacg ccagaaaccg tcgcagagtg cgaagcttcg catacggcgc gcttcctcaa
    31861 gccgatgctg taatcgttaa ggccgctttc agagcggcct tcttctttca gagttgcacc
    31921 agcaatttac gtttttcttc cggcagcaaa ttcaccgcct gctgataaga cgcatccacc
    31981 agataataga tttgcgaatc cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct
    32041 ttattcagat ggcggcttgg acgcacatcg ctgtgctgct gacgtagcag ctccgccagc
    32101 tccgggctgg ttttcagcga aacagctggg cgattttcta cttctttcac catcgcaaac
    32161 agcacatctt ccactttgat ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg
    32221 cctggttttg ccatgcaata ttgtagcaac tccgaaatgg tcatttttca ctccccttgt
    32281 agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat gttcccccag ccagatgcct
    32341 tgccaggtgc cggtctgaat acgcccttta tgcaccggca atacaagcga tgttcccagc
    32401 attgaggatt tgatatgaga aggcatatcg tctgctccct catagtcatg ctcataattt
    32461 ccgttatcgg gaacggcgcg gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg
    32521 cagttctcat taagtgtcag agaggcggag gtatgttgca gcaacagatg cagtaaaccg
    32581 atgttaacgc gcggcatatc agccagctga ttcagaattt catccgttac cagatgaaac
    32641 ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc acatgtgctg ctccttgata
    32701 aaactctctt aatcagtttg cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac
    32761 agcattcggg agagtattac gctctcccgc tctgtcagta ttctgaattg acgatcacct
    32821 cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa
    32881 tgcggatacc acgagcgccg acatcgcgtg cggcggtaat atcgttatcg gaatcaccat
    32941 aaaaaattcg gatattttta tcctgcagcc attgcgattt tgtattttgc cctggtttat
    33001 cgcccgcaaa gatcaccgga ttcatgttgg tggcaggaat atgaaaatta tccgccagcg
    33061 tttttgaaac cgtttctgtt ttggtcgggc tacgaccagt cacaaagaag atcgcgtcac
    33121 cgcggcgtac atgcatatca atcagctggc gagcgacctc ttttggaatg ctgaattcat
    33181 cccagccatt gttcattttt tcccagaaca caggattttt cagataatct tcgctttctg
    33241 gcgagaaggt ttttttgccg cgccagaagc ccggactgga aaaaagtacc gtgtcatcga
    33301 tatcaaaccc caccgccatt ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg
    33361 aaacccaatg aattggtgcc tgttcagcaa gcctggcaac gttagtacca gggttaagcg
    33421 gtgaaggaga tgaggccagg gcaacagcgg aactgtttag agcgaacaat aagcaaacgg
    33481 cactgattgc ctgtgtgatc ttgcgcatat ttttccctaa atagtcagta tgttgaaact
    33541 ttttattgtg agatttgttg caaaaaccat ctgaccataa cgccagcagc cggggaaagg
    33601 aaggattatc tgcggttttt gtgatgaaaa gcaggatgat tatatttaac ccagtaaata
    33661 cgttcctgtc gttttttgta aaaaaaatgt ttattacgcg atgtaagaat acattaattt
    33721 tgcgtttatt atgatgaatc ctctgc
//
LOCUS       DAZGAP010000028        33171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGAP010000028 DAZGAP010000000
VERSION     DAZGAP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.028"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(551..841)
                     /gene="nadS"
                     /locus_tag="ACHLAM_001144"
     CDS             complement(551..841)
                     /gene="nadS"
                     /locus_tag="ACHLAM_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO7852709.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(842..1153)
                     /locus_tag="ACHLAM_001145"
     CDS             complement(842..1153)
                     /locus_tag="ACHLAM_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7852710.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1382..2290
                     /locus_tag="ACHLAM_001146"
     CDS             1382..2290
                     /locus_tag="ACHLAM_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7852711.1"
                     /translation="MAQEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2354..3295)
                     /gene="fabY"
                     /locus_tag="ACHLAM_001147"
                     /gene_synonym="yiiD"
     CDS             complement(2354..3295)
                     /gene="fabY"
                     /locus_tag="ACHLAM_001147"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO7852712.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3340..3777)
                     /gene="dtd"
                     /locus_tag="ACHLAM_001148"
     CDS             complement(3340..3777)
                     /gene="dtd"
                     /locus_tag="ACHLAM_001148"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO7852713.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDCA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3774..4646)
                     /gene="yihY"
                     /locus_tag="ACHLAM_001149"
     CDS             complement(3774..4646)
                     /gene="yihY"
                     /locus_tag="ACHLAM_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO7852714.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4640..5239)
                     /gene="yihX"
                     /locus_tag="ACHLAM_001150"
     CDS             complement(4640..5239)
                     /gene="yihX"
                     /locus_tag="ACHLAM_001150"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO7852715.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5380..6267)
                     /locus_tag="ACHLAM_001151"
     CDS             complement(5380..6267)
                     /locus_tag="ACHLAM_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO7852716.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6257..7606)
                     /locus_tag="ACHLAM_001152"
     CDS             complement(6257..7606)
                     /locus_tag="ACHLAM_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7852717.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7705..9138)
                     /locus_tag="ACHLAM_001153"
     CDS             complement(7705..9138)
                     /locus_tag="ACHLAM_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7852718.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9208..10218)
                     /locus_tag="ACHLAM_001154"
     CDS             complement(9208..10218)
                     /locus_tag="ACHLAM_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO7852719.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10251..11138)
                     /locus_tag="ACHLAM_001155"
     CDS             complement(10251..11138)
                     /locus_tag="ACHLAM_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO7852720.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11163..12041)
                     /gene="yihU"
                     /locus_tag="ACHLAM_001156"
     CDS             complement(11163..12041)
                     /gene="yihU"
                     /locus_tag="ACHLAM_001156"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO7852721.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12214..13110
                     /locus_tag="ACHLAM_001157"
     CDS             12214..13110
                     /locus_tag="ACHLAM_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7852722.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            13150..13938
                     /locus_tag="ACHLAM_001158"
     CDS             13150..13938
                     /locus_tag="ACHLAM_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7852723.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13998..15263)
                     /gene="yihN"
                     /locus_tag="ACHLAM_001159"
     CDS             complement(13998..15263)
                     /gene="yihN"
                     /locus_tag="ACHLAM_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7852724.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15365..16345)
                     /gene="yihM"
                     /locus_tag="ACHLAM_001160"
     CDS             complement(15365..16345)
                     /gene="yihM"
                     /locus_tag="ACHLAM_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO7852725.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(16353..17063)
                     /gene="yihL"
                     /locus_tag="ACHLAM_001161"
     CDS             complement(16353..17063)
                     /gene="yihL"
                     /locus_tag="ACHLAM_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7852726.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVIPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17280..19103)
                     /gene="typA"
                     /locus_tag="ACHLAM_001162"
     CDS             complement(17280..19103)
                     /gene="typA"
                     /locus_tag="ACHLAM_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO7852727.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRSPKDD"
     gene            19475..20884
                     /gene="glnA"
                     /locus_tag="ACHLAM_001163"
     CDS             19475..20884
                     /gene="glnA"
                     /locus_tag="ACHLAM_001163"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO7852728.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            21058..22107
                     /gene="glnL"
                     /locus_tag="ACHLAM_001164"
     CDS             21058..22107
                     /gene="glnL"
                     /locus_tag="ACHLAM_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO7852729.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            22119..23528
                     /gene="glnG"
                     /locus_tag="ACHLAM_001165"
     CDS             22119..23528
                     /gene="glnG"
                     /locus_tag="ACHLAM_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO7852730.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23640..23750
                     /gene="yshB"
                     /locus_tag="ACHLAM_001166"
     CDS             23640..23750
                     /gene="yshB"
                     /locus_tag="ACHLAM_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO7852731.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23940..25313)
                     /gene="hemN"
                     /locus_tag="ACHLAM_001167"
     CDS             complement(23940..25313)
                     /gene="hemN"
                     /locus_tag="ACHLAM_001167"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO7852732.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25502..26011)
                     /gene="yihI"
                     /locus_tag="ACHLAM_001168"
     CDS             complement(25502..26011)
                     /gene="yihI"
                     /locus_tag="ACHLAM_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO7852733.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(26130..26296)
                     /locus_tag="ACHLAM_001169"
                     /pseudo
     CDS             complement(26130..26296)
                     /locus_tag="ACHLAM_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26593..27225
                     /gene="yihA"
                     /locus_tag="ACHLAM_001170"
     CDS             26593..27225
                     /gene="yihA"
                     /locus_tag="ACHLAM_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO7852734.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27549..30335)
                     /gene="polA"
                     /locus_tag="ACHLAM_001171"
     CDS             complement(27549..30335)
                     /gene="polA"
                     /locus_tag="ACHLAM_001171"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO7852735.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPVAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVVDEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            30699..31631
                     /gene="yihG"
                     /locus_tag="ACHLAM_001172"
     CDS             30699..31631
                     /gene="yihG"
                     /locus_tag="ACHLAM_001172"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO7852736.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCESLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(31672..33102)
                     /gene="yihF"
                     /locus_tag="ACHLAM_001173"
     CDS             complement(31672..33102)
                     /gene="yihF"
                     /locus_tag="ACHLAM_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFO7852737.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVKLVDNELTQPLFIAAKNKSPMEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDLVLELSPLNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQINIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLIFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8362 a         8481 c         7934 g         8394 t
ORIGIN      
        1 aaatcattta aaaaaagcgg atttttgcat gctgttttgt ttttgcgaga cgttttccag
       61 aaataataag aacgttgcag ttgtttcatt tgttaatcaa aaatggttgg gttatcgccc
      121 aggatgagat atgggtgcta taaatatatt atatacaggg atgtttattt tttgattgga
      181 tctttatttg cataatctga atggtgtagc cgaaaaatgt cagcatccta acagcacccg
      241 gatttaaggt gaaattaatc atccatcaca gaccggtcag tactgctacc aaccggtctt
      301 aacttgctca tcgttataat tttgttggta acagttcggg aaacaaggaa ttccttccgg
      361 aacacccccg gtatcatccg atagcattga cgtcaatcat cggcgcgacg atgagttttt
      421 ttaatcgcat catagggtgg caacggtcag cacgattacg ggtcatagca aaactcctga
      481 gcaggtggca ctacgccgct ttcacaggag ttctgaatgc tggtgctttc tgtatggcat
      541 aaccatgcct ctaacggtta agtgcttgca gcacatgttg cggatcattg gcaatcgctc
      601 gcagcaaagc ctttgcaggc ccggtcggtg aacgtcgccc ctgctcccag ttacgcaacg
      661 tgtctacgct tacagaaata agatcggcaa atttagattg tgacaaccct gaagcgcggc
      721 gtatttcttt gattttcata gcatcaactt caaaagtccg cgaaggctgt cgcgttcctt
      781 caataatttc attcatctgg gccatgctgt gagtcaaccg ctcaaaaagt gccttatcca
      841 tttaccacct cgtatttaac aaccggagca ttgctttttc ctggggagac agatcatctt
      901 taataccctt acgataaata agcagtaatc tgatctcata atgtttaacc tgatgaaagt
      961 aaataaccct gacaccagca cgtttcccct ttcctcctga cacccatcga acctttctta
     1021 acccaccagt ttcaggtatt acctcaccat agtcaggatt caaggccagg aaaaactgaa
     1081 aacgactgaa ttcatcgtcg gtcagcagtt tttgaacatc ttcagtaaaa atttccgttt
     1141 cgatgaagag cataattata cgccattggc ttagagtaac aatccttttt gtacgtcatt
     1201 gacgtaccct tgaaaccact agcttactga tagtgctcca gcaaaaccac gtctctgccg
     1261 ggtttgtctt tacatttgcg aggcgctttc cagatttttc acgcccgtga atttccctgt
     1321 atccatccag taaaaacggg tgtataaaac tgtacattca tacagcaaaa tgagttctac
     1381 tatggcacag gaaaaaggaa ttgcagagtt agtcgaagag tttatcgctg cgggtcgccc
     1441 ctcatcgcgt caacaaagta ttgcgcagcg cagggagggc tatatcgcca gcgctgtcct
     1501 tgcgggtgag acagaaacgc gggtagatat tcagactatc gaacttgaag cgatgacctt
     1561 acgaatagtg tcaccgctga atgccccgac attactcccg acgataattt attactacgg
     1621 tggctgtttt gttagcggcg ggttcgcaac tcacgacaac cagctgcgcc agttagccta
     1681 ttacgggcaa tgtcgggtta ttgccgtcca gtatcggctg gcaccggaac atactttccc
     1741 cgccgcccat gatgatgcgc aacgaggtgc agaattagtc tggcaacacg ccgaacgact
     1801 cggcgttgat aaacaacgga taactcttgc cggtgacagt gcgggcggac atctggcgct
     1861 ggtaactgct ttgcgattaa agcgtgctgg cgagtggcaa ccggcacaac tcatcttaat
     1921 ttatcccatg cttgatgcga ctgcgcattt cgaaagctac atacgcaatg gtcacgatta
     1981 catcatcaca cgcgacactt tactgagcgg ctttgagatg tacctgccag gcattgaacg
     2041 caggcatcct gaagccagcc ctctttggcg cgatgatttc aatggcttgc cacctgtaca
     2101 tatcattacc gctgaatacg acccactttg cgatgaaggt gaagcgttgt atcaccgcat
     2161 gacagggcaa ggagtgcaat gcacctgcca acgctacctt ggcgttatcc acggattttt
     2221 tcagttaggt ggtatcagtg aggctgcgcg atcggcgcta cgggatgttg cctggagagc
     2281 gggaaggtaa ctccggacca gggcccagaa aaggttttca gattgaaaac cttttcagtc
     2341 aagtaaaacg cccctactct tcttcgttcc cgccctcttc atacgggcca aatggcttcg
     2401 cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg ccaaagattt
     2461 ctacttgcat ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga tcgccgctta
     2521 aggcaccaag gtcggctacc gcatgaggtt tgccgctaat cggcttgctg tagcggatat
     2581 gcgcatccgc cagaataatc gttccgccga ggtggcgttc gcgcagcatc agccagataa
     2641 gcccccaacc ggtgagcgtt gccagcgaga ataaactccc ggcaaacagc gtgtgatgcg
     2701 gattctgatt gcctgtttcc ggcatggtgg tgataaattt ttgcccggta tattgctgaa
     2761 tgcgcacgcc cattttttca ctcagcggga tgtgttcata ccacgcctgt tgcagctgcg
     2821 cgcaccagtc gccgcgatgc aaaatatcat ccagagtggc aacgggttta atcatcaaaa
     2881 aatggcgaat cggcgtggtg gttggcgtgg tgatttctcc ctgattaaca aaccccagct
     2941 tggcgaaaaa ctccaccgcg tcttcgcggg cgctacaggt cacgcgctta acaccttcct
     3001 gacgcgccac agattcgagg gtcatcgcca tcagcgttcc taaccctttg tcctgcacgt
     3061 cgggatgaac ggccataaag cgaatggacg cttcattgtc ggcattaata tacagtcggc
     3121 ctaccgccac cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc atcgcatccc
     3181 acgcgtcgcg ttccgaacct tttggttgat gcaggggctt acgcaacatt tcccagcgaa
     3241 actgatagta acgctctaat tcttcttctg tttgtggaac ccgaaggtga tacatagcga
     3301 tactctctct tgtttcccgt gacaaccctg gaagctggct catacctgca accagaatgt
     3361 cacggggcca tcattgacca gcgatacctg catatccgca gcgaagcgtc ctgtttgcgt
     3421 gttcatctct tgctgacggc agcgttcgac gaaatagtca tataacgcct ctgcgcaatc
     3481 cggtgatgcg cctttggaga aacttgggcg catcccccgt tcggtatctg cggcaagggt
     3541 aaactgggaa accaccagta cactgccgcc cgcctgttgc acgttgagat tcatcttgcc
     3601 ttcggcatcg ctaaagatgc ggtagccgag cacacgctcg cacagacggt ttgctttctg
     3661 ttcgtcgtca tccttttcga cacccaataa caccaaaagt cccgcgccaa tttcacccgt
     3721 cacttctccc tccacggtga cgctggcacg ggttacgcgt tgaattaatg caatcatggt
     3781 tcgtcgtctt cttcttgttc agcagcttgt tttaatttgc ggtattcccc gagagtgaca
     3841 gtaatttccg cgccaagcaa gacgatacac catgtccagt agacccaaac aaagagaatg
     3901 gggatcaccg ccagcacacc gtaaatgagc tgatatgatg ggaacatggt gatataaagc
     3961 gcgaaacctt tctttcctgc ttcgaacagg agcgcggcga caaacgcgcc gacaatcgcg
     4021 tcgcggttag gtacacggat ggtaggaacg atgctgtaca gcagccagaa agagatccac
     4081 gacaacagca gcggaaaaat acgcaacaca ttatcgatga cagtattgag atcgctcgcc
     4141 cagcgcagag agagcagata ggagctgatc gccagactgg cccctgccaa cagcggcccc
     4201 agcgttaaaa tcatccagta cacggcaaac gagtaaattt ttggtcgcgc tcgtttactg
     4261 cgccagatgg tattcaacgc gctatcgatg gagtacatca acaataacgc cgtgacgatc
     4321 agcccgcacg ccccgacggc ggtcattttg ttggaattgg cgacaaactg ttcgatatat
     4381 cgctggataa catcgccagt agcaggcaga aagttggcaa aaataaagtg acgcaactga
     4441 atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac ggcaaccagc
     4501 ggcactaatg agagcaacga cacataggca aggttacctg ccagggttgt catgttgtcc
     4561 tcatcaatgc gttgccagag tagttttagc caggcccata gtggacgggt acgatgcctg
     4621 gctttgtcct gaatggtttt tagcataaca ccttcgcgaa atagtccggg atggtggttt
     4681 tatctttcac cagaatacta gtaatgccta gctgattagc tccttctata ttatcggcgt
     4741 tatcgtcgaa aaagaccgta tcgtctggtg aaaaaccttc tgcctgcaaa acatgctgat
     4801 aaattcgtgc ttcaggtttg cgcataccca agtcttgcga cagatagata tggtcagcag
     4861 catcacggat ttccgggtat tcctccggcc agaaggtagt atgcaggcgg ttagtattgg
     4921 aaagcaccac cacgcgatgc ccctgctcac gcagtttatg catgatggcg atcacttccg
     4981 ggcgcagcgc aacaaatacc gcctgccaac cgtgggagaa ctgctcatag cttagcggca
     5041 gggccatctc atggcacagt gcctctgcga acgcttcgtc gctaatttcc ccacgctcat
     5101 gctgatgaaa cgcctcgccc atgtggaaac tcttcttaag cgtagccagt ggaacacgcg
     5161 ttaaatcgct ccaggccccc agcacacggt taaagtcgat atcgacaatc acattaccta
     5221 aatcaaagat atagagcatt tttgcctcct ttcgcgccac aagaaattaa ctgtagcggg
     5281 aaaggatggg tttggctatg tggcaataag ggtaatcttg tgatgctgat tgcgaaaaag
     5341 cagagtaatt tcactggctt tttcgcaata gatgaatggt taagattcag gtgtaaattg
     5401 ccaacgagtt tcgatgacca gcggcttatc tggtgtcaca aattccggcg ttagatttat
     5461 tgcgttaggt gcctgagtca gcgggttaat acaggtggca tcgggctgtt tatcaaatac
     5521 caccagactg tttgcagaag aggtcatggt catcgcgagt ttaccaggcc acagtaactt
     5581 aaccttcacc ccgtcataaa aaccaaaaca gtcatcccag ggaccgactt gaggagagat
     5641 acgatttcca gttggcagtt catcactacc cgcttcttct tgccagtccg catcaaaaag
     5701 cacctgcaac gattctgtat tttgcggagt cagctttttc gcaaaccacg gatgccagcc
     5761 tgcggaggcc gggaatgtat cggcatatga atggacttcc agttgcaaaa ccagcgcatc
     5821 gttctccagc aaaaaagtct gaatcacttc cccttgccag ggccacggtg aagccagggg
     5881 catacgtaaa gtcagggaat ctgctgtttt gtcgatgatt tcccacgttg agtaacaagc
     5941 cataccatgc aacgcatgtg gtggtttatt ggcgggtaaa gaataacatt gcccaccagc
     6001 attcagcgtc gcattcccca gtcgccctgc ccacggcacc attgggaaac agccatactg
     6061 aaaagctctg cgctgcggct gccattggcg taatacttca gaaccaaatg cctttaacga
     6121 ggttatcctg gcaccatcat gcggataaat ctgcaggtga atattgcctt tttccagcgt
     6181 cactaactct cctacaggcc caggccatgg cgcgtcaaac gcctgttgta gcgccaggta
     6241 atgctcgttt tttcgttcag ttttcatgag attgttccct gatgtagcgg taagaagcgg
     6301 tttcttgttc atcggttggc tcaatcagcc agacgcaaac aaaggaaaga actgagacag
     6361 caaataaaaa cagagcgaca taccacggtt ttcctccccc tagcgccagc aaacttgagg
     6421 cgataagggg gcttagtccg ccaagaatcg cggaaaattg ataagcaaaa gaaaggccgg
     6481 tatatcgtac tttggtaccg aacatgcggg tgaaaagtgt tggctgcacg gcaaacatca
     6541 tcgtcggacc taagttgtag ccaaggatca tactgcacca gatgatgagc gtacttttgc
     6601 tatccagtaa taagaagaaa ggaaacgcga acaggacaca aaatcctgct cccgacaaat
     6661 acagactttt ttgacctata cgatctgata accatccaac caatggacaa ctgaaaatac
     6721 cgatcgccga ggcaatcaac atacccgtca ggggaatatc tcgcgacaat gctaattggc
     6781 tggaaatata gacaatacca aaggcgttaa taatattaga ggagacactt tccgccagcc
     6841 tcgcacccag tgccaataaa atatttcgtg gatgtttttt gaataattca atcaacggag
     6901 gaacggactt ttctttatta ttcgtcgttt tttgcttttg aaaatccagc gtttcttcgg
     6961 tatgcaggcg gataaacagc ccaacgatca acatcaccgc agaaagccaa aacggaatgc
     7021 gccatcccca ggagagaaac tgctccgaag taagaaaatg gttacagaga gcaaaaatac
     7081 cggttgccag cataataccc acagacgcag ccgtttgcgg gagtgaacct aaaaatccgc
     7141 gtcgggattc gggcgctgat tctatggtca tcaatgccgc tccgccgtac tcgccaccaa
     7201 gaccaaagcc ctgaatcaaa cgcagcacca ttaatacaag cggtgcccaa atgccaattt
     7261 cctgatacgt cggaataaaa ccaatcaaaa atgtggagca accgacaata gccagcgtcc
     7321 agataagtgt tatttttcgc ccgattttat cgccaaaatg accaaagacg atgccaccta
     7381 aaggacgagt cagaaaacca acaccaaagg tcgcaaagga agctataaga cctataaaag
     7441 gatcgctgtc tggataaaac agcggtgcaa ataccagtcc tgctgccgtg ccaaaaataa
     7501 agaagtcata ccattccatt aacgcgcctg cgaaactgcc cataacgaca cgtctcatgg
     7561 ctttaccgga aagcccttca cgcggtgcgg gtaacttttc agacataatt ttcctctcct
     7621 ggtaggcgag aaatctggga tacgaaccac taaacgtggg cagtttgaat taatgcgcat
     7681 cggcttaaac cgatgcgcat taaattattt cttcaattca gccagaacct ctgcgtcctc
     7741 agcatcaaca tttgcaccca gtggttccca attaatcaat acacaggtca ggaatcccag
     7801 gaatggcacg attgccaacc agtaaaacgc ttcagttttc aacgcacccc acagcatcgg
     7861 ccagaaaatg agtcctgcaa ttgcccctgt gcgcataata gcgcgggcaa atccgacccc
     7921 agtagggcga atagcgggag gataagagag tgtggcaatg gtcatgccca gcccaccagg
     7981 acccgctgaa tggaagaaaa taattgatcc caataaccct aaagacagcc agggattagt
     8041 cgttgccagt gcgcctaacg acagcaatgc taaggtcaca acagcaaatc catacatcga
     8101 ctgccggcga gtacccagtc gttggagaat aaacgagccg atcatcccgc ctgtcacccc
     8161 acaaagggca ttcacgacgg cagaaccggt taacgcgcca gtaagcccgc cacttattat
     8221 tcccgccaac gtaagaggaa gataaacacc tacggcgtta tattgccacg cctgcatggt
     8281 tgccacgaca cagccaagaa tggtgcgttt gcggtaacga tcattaaata agatgccata
     8341 tccaccactg tatttattct ctgctttttc cgagaatttc gcttctgttg tacccgatgc
     8401 cgcaacgcga gcctgaacac cataacgttt tcccagaatg tcgcacgctt cctggtaacg
     8461 cccgacgcgt gcggcccaca ttgcggattc accaataaag aagtaacgca aaatcatgaa
     8521 gatgactgca aaaatagctc cgatgaatat tccgtaacgc cacaattgtt cttcagcgat
     8581 acccgacaga agcagcggca aaagcaccaa ataaacaacg gttgtcgaaa cataccaggc
     8641 catttgccag aggttgaccg acgtgccggt tttcttcgac gttgaaccac gaagctctgc
     8701 cacggcattg gttgccaaag ggaagtcgat gcctaaacca aagcccatga tgacgcggca
     8761 caccagcacc caccagatac tgggagcaat agctaccgct gcagcaccga tggtgcacag
     8821 ccccatgcca ataataaatg cccgcttttg cccaaagcgg tttattactc ggttcgctaa
     8881 taaaccgcca attaacgcgg cgattaatac tgatgcattg acggttgcag caagacccgg
     8941 cgaaatgccg aattgagcgg caatgtattt attaccaaac cccaccatcg ccgcctggta
     9001 ggcatcaatc aatatgccgc ccaaagcaat aaaaataacg ccgatactgg tgttgattgc
     9061 agagttctta ttaacaatat caattacgtc ctgtggccga ctaattgaat aagttgcagg
     9121 actggtgatt tgactcatcg cagtgtcctt tttaattgtt gtagggtggc gctgacgtga
     9181 cgcagcgcca gacagagatt tagaaaatca ttccggcgca aattgcagca tgacctttat
     9241 ggagtcattt tgctgcgcgc gttcgaatgc cgccgctgca tcctcaatgg caaataaatc
     9301 ggtaaccagt tcactggtat caaaccgccc atccgccagc cattcgataa cagcatcgaa
     9361 atcttcaggg atgtacatgc cagagttgag caagtcacgc tcgaaacgtt gcatcatcgg
     9421 taatggtatt tcacgcggcc cacttggtac gcccatgcac acgacggtgc ctccggcata
     9481 tacacgggta catgcatcgt taagcgtggc ctgcgctgca acgacatcaa tcgcccccgt
     9541 aaatcgcaca tctgcggcca gctcacctgg tgcccaaact tctgctgcgc ccagttttaa
     9601 cgccagcgcg cgtttggcag cgtcaggttc aatcacggta atcttacctg cccccataat
     9661 gcgcagtgcc tgcacgatgg ataagcctat cgttccggca ccaatcacca gtacgctttc
     9721 cagagaagct ttcggcattc ggttaacgca gtgacgagca caagccgccg gttcggcaaa
     9781 ggccaacact tttagcggta aagaggctgg tgcgacatgg caattacgcg caggaacaat
     9841 gtgttgtgag cgagcaaagc ccggtgcgcg gaaaccagca acctgcggtg gttcacaaag
     9901 attaaaacgt cctgctttgc aggctcggca ttccatgcaa gccatgatgg gatcgaccac
     9961 aacatgatcg cccggctgta catttttaac gtcgctgccc acttccgtaa cgcgcgctgc
    10021 aatttcatga ccggggacga caggcggttt ggcaaacgga tgaccgcctt tcagaacatg
    10081 cagatccgaa ccgcatatcc cgtagaacac gggcgcaatc cgtacttcgt gcggtttaag
    10141 ttgctggtgt ggtacttcaa catattgagt ggtgactttc ccaatatctg aaaataaaac
    10201 ctgggtaatt ttatccattg ttattctcca gaatcgatat ccatagggga ttagcgttgc
    10261 gcgatacctt catcgacaat ttcagccagc cgttgcaagc gtggaacgga gacatcgcgc
    10321 agcatggttt gtggatcttg tgcaccaaca accgacgccc aaaccgctcg tccagccagg
    10381 aagcccgacg caccaccttt catggcaata cttactgcac ggccaaaaat atcggcatca
    10441 acgccagaag aaagaattac ccacggcatc ttcatctgat cgttaagttg ctgacaggca
    10501 gcaagtaagg ttttttcatc gcctttgccc cctaatggca tctcggcttt gtaaagatca
    10561 gcctctgttc caccaagctc tgcggcggcg gcaacaatgg cgctttcacg atcaaagtcc
    10621 caaccgcgac gaggtggacg caccacaggt tcaataatgc taaccagccc ggcactacgg
    10681 cagcgacgaa caaatttatc aaccattgcc agacgttcct ctgcgggttc atcttcacgc
    10741 cagagtacaa gtaatttcat tgccttagcc cccatttcac gggctttatg cgggtcgacg
    10801 gacatatcta tttcaacgct atcaaccggg atgccattac ctgggataaa aagatcggca
    10861 gcgacaatca ggccgcaaga attagccaca gcgccctgct cgactatttg ttcaagacaa
    10921 aattgtttat ccgccagaac ggcagaagca taaggagaaa ggattcgggt cgcggcaact
    10981 ttaaaatcgg tgagtacact gtcggcgatg ggttttggct gccctgctgc cgcaaacatc
    11041 aggcgcatgg cctcacgctg atcgacagct aacatggcga atccaccacc tgggcgggag
    11101 atatctttca gcgtaaatgg ggtgaagtgg gatgacatag aaactcctgt taatagataa
    11161 aattaaagac cagcctgctg gcgaacctga tttagcaggg cgctccagtc ctgccgccct
    11221 ttgccgtctt gacttgctgc ggcgtaataa ctttctgctg caatccccat attgagcggc
    11281 gcccccgttt cccgagcgac gtcaactgca atacgcagat ctttcaatgc caggtccacc
    11341 atgaaggcgg gagaaagatc gcctttcagc actttgccag gccaggtggt cgtaaaatgt
    11401 cctttccctg ctgccgtacc actcatcacc ttaattgcga catcaagatt taatcccagt
    11461 gactcacata acacagcggc ttcggaggac agcgcattaa gcgcaatgct catatagttg
    11521 ttaattagct tcacgcggat ccccattcct gggccaccag cctcgaccag ctcattaccc
    11581 atgcacatga gaatcgggcg tgcacgcgca atctgctcat cagtgccgcc cgctaaaatc
    11641 agcaatgtgc catcaatcgc attcactgac gttctgccca cgggggcgtc catcatattg
    11701 atgccttttt cttgtaattc ccgtattaat gcatcggtct gtaagggatg aatagtcgac
    11761 atatcaatca ctaacgcgtc agatgaaaca gtttcacaga cgcctttttc ccccaacagc
    11821 acctgtcgaa caatgtcccc attgggaagc atagtgatga taaattcagc gcttgctgcc
    11881 gcttccgctg gggtttgtgc cgcagtggcc ccctgcgtaa ctagcgcatc gacggcctgt
    11941 gtattgacat caaatacctg taatgaatgc cctttttgca atagattgtt agccattggg
    12001 ctacccatct gccccagccc gagaaatgca atggttgcca tgaatactct cctgtctgac
    12061 attgacaaaa taatgacaat tgaaaattcg ttttcgtctg tttttgagca tatttgtaat
    12121 ttatgttcaa aaacacgaca cgcatcacta atttgatcat ttgtgtcatt taaattgacc
    12181 aaagacgaca aaattaaaca ggacaagaca aatatgacca gaattgcatg cgtgggtatc
    12241 acagttcagg acaggattta ttctttgccc actctgccgg agggcggcgg gaaatatcag
    12301 gcaaatcatt atcttgagat tggtggcggc ccggcagcaa cagccgctgt tgcaatcgca
    12361 aagcttggcg tggaggttga tttcatcggt cgcgtcggcg atgacagttg tggaaatacc
    12421 ttacttgcag aacttgaggg ttggggcgtc aataccgcat tttgccgccg atatcctaac
    12481 gcccgttcgt cgcaatcagc gatcctcgtt gatcagcatg gcgaacggat tatcgttaat
    12541 tatccaagtc ctgatctcgg tactgatgcc gaatggttag aagcgatcga tttctctcgt
    12601 tatgatctga ttctggccga tgttcgctgg catagcggta ctgaaaaagc attctcgcta
    12661 gcgcgccttg cgggtgtgac aacgctttta gacgccgata tgacaccgca ggatatttct
    12721 ccacttgtcg cgcttgccga tcacgccgtg ttctcaacac ctgggctgaa acgcatgact
    12781 ggcctgcagt cacctgaaga aggtttgttc caggcaacga cacaaacagc aggtaaagtg
    12841 tatgttacgc tgggttctga aggatcgttg tggattgaag atggtcattt gtgtcaacag
    12901 gaggcatttt ctgtcaatgt ggttgatacc actggagcag gcgatgtttt tcacggcgca
    12961 ctcgcggtag cgttggcaga aaaaatgcca acaaaagagg ctattcgttt tgccagtgcg
    13021 gttgccgcga tgaaatgtac ccagcctggc ggtcgggccg gcattcctaa tcgtgagcaa
    13081 accgaatcat ttttgtcact ttatgcgtaa aatggtaaac atctaccgag gaggggcaag
    13141 ggagtattca tgagtatcat cgaagtgaca ggtaatccac gccatgacca actcgttcat
    13201 ctaatcgcag aacgcggtta tatgaatatt gaagaactgg cacaactgct ggatgtttca
    13261 acgcaaaccg tgcgccggga tatccgaaaa ctcagtgagc agggcttgat cacccgtcat
    13321 catggagggg ccgggcgcgt ctccagcgtc atgaataccg cttttgagca acgggaactt
    13381 tcgctcaccg ccgagaaacg ggcgatcgct gaggcagtcg ccgattacct tcccgaacgc
    13441 tgtaccgtct ttatcaccat cggtacaacc gtagaagccg ttgccagggc attactcaac
    13501 cggcgtgatt tacgcattat cactaatagc ctgcgtgtgg cacagattct ttataagaat
    13561 caggatattg aagtgatggt gccgggaggc actttacgcg ctcataacgg tgggattatc
    13621 ggcccaggag ccgtggattt tattgaaggc ttccgcgcag attatttaat caccagtata
    13681 ggtgccattg aacatgacgg caccctactg gaatttgatg tcaatgaagc gttagtcgcg
    13741 agaacgatga ttaaacatgc gcggaataca ttgttagtgg ccgatcatac gaagtttacc
    13801 gcgtctgctg ccgtttcaat tggcaatgca cggaatgtca gggctttttt tactgatgcc
    13861 ccgcctccca attctttctg ccagttgtta agtgaagaga atgttgaact ggtggttgcc
    13921 gagcaagaag tatcctgaaa ttgaaacttc ggttgatacg gttttcgttg gccggattaa
    13981 gcacatccgg ccactaatta agcttcagcc gccagcatct ctttcttacg ttggctaata
    14041 cgcttaacca gcattacgga aaccaccgca ccacagaatg cgaagcaggc cataatgcca
    14101 aacacgattt tgtagccaat aatccccgga tttaaatcca gaatgtagcc atacagactg
    14161 aagcagaaca tcgccggggc gtaaccaata aagctaccca acgccatcgc cgcgcctgtt
    14221 ttattttcag caatttttgc ttcgccgata ggtgcaaaaa atacggctcg ctgtgtgaag
    14281 actatagcgc caaagcccag cgtacatgcc atccctaaat agaccggcat actttcgtgc
    14341 ggcagcataa tcaacagtac gagcgcagcg gtacttatga taaaggtgta gcatagatat
    14401 ttactcggcg atttcaggat cttatctgaa atcatgccac caatcggtcc tccaatcatt
    14461 ttcagacagt attggttaat gatgccgtaa gcccccacca gagcaacggg caatagatag
    14521 atgtttttca ggaatggaat gaagaatgtt aggccacagt aaaccgcgta aacgaagaag
    14581 acgttgaaag cgataagcca gatggtttta tctctcagca ccgaggtcat actggtgttt
    14641 tgcgatgctc cgtcttcttt tttcacctca acggacggtg cctcttcttt gtcattcagg
    14701 acaaagaaaa tgataatgcc tacggcaatc acaataaggg aatagaacca gatgcctgct
    14761 ttaaaaccca ataagccact gccaaaccag gtaaatactg ccaacgcaga aaatgccacc
    14821 acggtatcga caatgccacg ccctgtttcg aagaagccaa acaaccgacc ttgttgttcg
    14881 ctattgccca atcgacttac cgacttgagc aagaccggcc agttcatcat gtcgcaagta
    14941 acgccaaata gcgcccagac aaagaggatg ccccaatacc ccggcattgt cgtcaaataa
    15001 acacccagta atcctgtcgc aatgagtgag aatgacatgg tgtatctgcg cggtagttta
    15061 tcggcaaaat aaatagacag aaaaaagccc actgtggtga caaatgagtt taccgacata
    15121 gcattaccaa tttgaccatt ggtcaaatgg aaatattcct gcatcgggat ataaaacgca
    15181 tctttcagag acggtaattt ataaattgta ccgccacaca atgttaatag actaaataac
    15241 gcccatttct ttttcgtgag cataacatcc cctaagcgat ttttaaaatc actgttttaa
    15301 aggcaataag ataccgacca ttcatatgga atggttttat taacagcgta tgagttaaaa
    15361 cttattagtt gttcagaaga tggtttgcct cttgtttgat ttgttgtagt acattacgca
    15421 catgatttat ctgattaatt gcatcttctt tttctttacg taaccgcgca tccagtaaat
    15481 gattttctgg caatggtgtt tcactcatct ggcgataagg gatccacgga ttatcatctt
    15541 cgtcttcaaa gcggaacgcc ggcgcatagt aatcaacctc ttcttccagg ccatatgccg
    15601 tcacctgcgg ctcatcatca cccaggcaga taaggtgcgt taataacgct ttgaacggca
    15661 tatcaccctg acctgaaata caggctttat gacccaggcc accctcttct ttaacgatca
    15721 aggcatcttt gatatggacc tgggtaatat gcggtgccat cgtttttaaa gcgtcgatgg
    15781 gatgctcatt tgcattgatc atgttcgcaa aatcaaacag taaggaaaga gattccatct
    15841 cactttcttt gaccagcgac accagctcat ggctttttaa atcttcatgc tgttcgatcg
    15901 taaaagtcag gccgctgtcc tgatacgttt cccgtacata ggcaatgtcg ttagcgatga
    15961 tcgataatac gtcgcgcaaa ttaccttcat aacgtggata aaaacgtacg gacgatgccc
    16021 cagttttcaa cgcaatggcg acggcttcgt caatagatgc cttatctgaa gcgctggttt
    16081 caatatgaat atcaaggttc agacggcggg ctttatcccc aaaggcagag agttctttat
    16141 cgtccatatt accaagagaa aaacgctcgc catccagaac atggattttg acgccacgta
    16201 gattattttc acttgcgata tcaagtaaat cagcaggcaa cactctttcc agccgcatat
    16261 ttaaatgaaa ggcataagca tgtaaataaa gaggaagagt gtcgacacgt tgtagaatct
    16321 ttcttgcgtt atttattgtc accataaaac acctacagat taatgatgag gtctacggcc
    16381 tcaaatcgcc aatattcaat atctatctgg acaatattgc cttgctggtc ggcgcgatgc
    16441 ttttcaatta agatcgctgg catacccgca gaaccgccaa gatacttgct gatatcgccc
    16501 ggcattctga cgggtttgaa aatcaatttt tttactaccg tctctttttg gtagcacttt
    16561 tcccagactg cagaaaatga gtggttttca agttgttcaa taaaacccgg ggcaacttca
    16621 ggattaatat atgtttcgtg atagaaaact ttatgtcctt ccagcgctcc ccagccggtg
    16681 atgcgataga gttcagttga tggtatcact gtaatcagtg gcacgagcgt ctcgggaata
    16741 tctctggtac ggcttttctc ggtaaacccc caggaaggct cccgcccttg ctcaattgca
    16801 gcccgctgaa agctcgccga cagctctgga ctgtaattaa atcgcggctg ggtcacgaac
    16861 caccctttac gatccttacg gaagattttg gattctgcct cgaggttcaa caacgcctgg
    16921 cgcagcgtca tacgtttaat gcccagcagt tctcccagct ctctttctga agggagtttt
    16981 ccacccggcg tggtgatgcc atctttcaac caccgatcca gtttctcttt tgcattttct
    17041 acggtggatt gattctccgc cattgtttaa tccttttggt ttaaaccaga tgaggcatct
    17101 tacgcttacg ccaaaaaaga aagcaattaa cggcgtatga ctttgcagat aattgtgaca
    17161 aagctctcta ttgacgtaat cacagactta taagggagat actcgcattg gtgtgcgctc
    17221 acgacaggta aaaaaaaacc tgccagcgat ggcaggtttt ttatgactga aaagcgaaat
    17281 taatcgtctt tcggtgagcg gttggcgcga cgacgatcgt tttccgtcag gtgacgttta
    17341 cgaatacgga tagaggtcgg agtcacttct accagttcgt catcatcgat gaactccaga
    17401 gcttgttcca gagtcatgcg gataggcgga accagaacaa cggcttcgtc agtaccggaa
    17461 gcacgcatgt tggtcagttt cttaccggtc aggcagttta cagtcaggtc gttagagcgg
    17521 ctatgaatac cgataatctg accttcgtaa acttctgcac cgtgaccgag gaacagctta
    17581 ccgcgatcct gcagaccgaa cagcgcgaac gcgaccgctt taccctgacc gttagagatc
    17641 agtacaccgt tctgacgctg acccacttcg cccggacgta cgtcgtcgta gtggctgaag
    17701 gtggagtaca gcagaccagt accggaagtc atggtcatga actcagaacg gaagccaatc
    17761 agaccacggc ttgggatcac gtagtcgaga cgtacgcggc ctttaccgtc tggattcatg
    17821 tttttcaggt cgcctttacg ttcgcccagc gcctgcatca cagaaccctg atgctgttct
    17881 tcaacgtcca gcgtcacgtt ttcatacggc tcttgtttac gaccgtcgat ttcacggaag
    17941 attactttcg gacgggatac cgccagttcg aaaccttcac gacgcatgtt ttcgataaga
    18001 acagacaggt gcagttcgcc acgaccagaa acgcggaacg catcggcgtc ttcggtttct
    18061 tctacgcgca gcgcaacgtt gtgtaccagt tctttgttca gacgatccag gatctgacga
    18121 gacgttacga acttaccttc tttaccgcag aacggcgagg tgttaacgca gaagaacata
    18181 gaaacggtcg gctcatcaac ggagagtgcc ggcagcgctt caacgttttg cgtgtcgcaa
    18241 acggtgtcag aaatgttcag ttcgcccagg cccgtgatcg ccacgatatc gccagcttcc
    18301 gccagatcgg tttcgatacg ttccagaccg aggtggccca gcactttacc gactttcgcg
    18361 ttgcgggttt tgccttcgct atcgatgata gtaacctgct ggttcggctt cactttaccg
    18421 cgcttgatgc ggccaatgcc gataacgcca acatagctgt tgtaatcgag ctgagaaatc
    18481 tgcatctgga acggaccgtc aaggtcaacg tccggcgcag gaacgtggtc aacaatcgcc
    18541 tgatacagcg gggtcatgtc ttccgccata tcttcgtggt ccagacccgc gataccgttc
    18601 agcgcagaag cgtaaacgat cgggaagtcc agctgctcgt cggtcgcgtc gaggttaacg
    18661 aacagatcga atacctgatc cacaacccaa tcaggacgcg cgccagggcg gtcaactttg
    18721 ttgataacaa caatcggctt caggccgtaa gcaaacgctt ttttggttac gaagcgcgtt
    18781 tgcggcatcg ggccgtcaaa tgcgtcaacc accagcagca ctgagtctac catggacatt
    18841 acacgttcaa cttcaccacc gaagtcggcg tgccccgggg tatcaacgat gttgatacgg
    18901 taatcattcc atttgatagc ggtgtttttc gcgaggatgg taatcccacg ctctttctcc
    18961 aaatcgttgg agtccatcac gcgctcttgg gtttcggcac gagagtcgaa cgtaccggat
    19021 tgttggagca gcttgtctac cagggtggtt ttaccatggt ctacgtgcgc gatgatggcg
    19081 atattacgca atttttcgat cacaactttg cctcaggcat tagaaatagc gcgttattgt
    19141 acacggatta atcgcactac aaaacaggat cacaaacatc ctccgcaaac aagtattgca
    19201 gagtcccttt gtgatcgctt tcacggagca taaaaagggt tatccaaagg tcattgcacc
    19261 aatatggtgc ttaatgtttc cattgaagca ctatattggt gcaacattca catcatggtg
    19321 cagccctttt gcacgatgtt aagcatgata acgcctttta ggggcaattt aaaagttggc
    19381 acagttttcg ctttatcttt ttacggcgac acggccaaaa taattgcaga tttcgttacc
    19441 acgacgacca tgaccaatcc aggagagtta aagtatgtcc gctgaacacg tactgacgat
    19501 gctgaacgag cacgaagtga agtttgttga tttgcgcttc accgatacca aaggtaaaga
    19561 acagcacgtc actatccctg ctcatcaggt gaatgctgaa ttcttcgaag aaggcaaaat
    19621 gtttgacggc tcctcgattg gcggctggaa aggcattaac gaatccgaca tggtgctgat
    19681 gccagacgca tccaccgcag tgattgaccc gttcttcgcc gattctaccc tgattatccg
    19741 ttgcgacatc cttgaacctg gcaccctgca aggctatgac cgtgacccgc gctctattgc
    19801 gaagcgcgcc gaagattacc tgcgttccac tggcattgcc gacaccgtac tgttcgggcc
    19861 agaacctgaa ttcttcctgt tcgatgacat ccgtttcgga tcatctatct ccggttctca
    19921 cgttgctatc gacgatatcg aaggcgcatg gaactcctcc acccaatacg aaggtggtaa
    19981 caaaggtcac cgtccggcag tgaaaggcgg ttacttcccg gttccgccgg tagactctgc
    20041 tcaggatatt cgttctgaaa tgtgtctggt gatggaacag atgggtctgg tggttgaagc
    20101 ccatcaccac gaagtagcga ctgctggtca gaacgaagtg gctacccgct tcaataccat
    20161 gaccaaaaaa gctgacgaaa ttcagatcta caaatatgtt gtgcacaacg tagcgcaccg
    20221 cttcggtaaa accgcgacct ttatgccaaa accgatgttc ggtgataacg gctccggtat
    20281 gcactgccat atgtcgctgg ctaaaaatgg cgttaacctg ttcgcgggcg acaaatatgc
    20341 aggtctgtct gaacaggcgc tgtactacat tggcggcgtc atcaaacacg cgaaagcgat
    20401 taacgccctg gcaaacccga ccaccaactc ttacaagcgt ctggtcccgg gctacgaagc
    20461 accggtaatg ctggcttact ctgcgcgtaa ccgttctgcg tctatccgta ttccagtggt
    20521 ttcttctccg aaagcacgtc gtatcgaagt acgtttcccg gacccggcgg ctaacccgta
    20581 cctgtgcttt gctgccctgc tgatggccgg tcttgacggt atcaagaaca agatccatcc
    20641 gggcgaagcg atggacaaaa acctgtatga cctgccgccg gaagaagcga aagagatccc
    20701 acaggttgct ggttctctgg aagaagcact gaacgaactg gatctggacc gtgagttcct
    20761 gaaagccggt ggcgtgttca ctgatgaagc gatcgatgcg tacatcgctc tgcgtcgcga
    20821 agaagatgac cgcgtgcgta tgactccgca cccggtagag tttgagctgt actacagcgt
    20881 ctaatagttg aagttgtact acccggcgca acaacgccgg gatttagttg ccgtggaaac
    20941 tttcagccca tctctgcatg ggcttttttc tccgtcaatt ctctgatgct tcgcgctttt
    21001 tatccgtaaa aagctataat gcactaaaat ggtgcaacct gttcaggaga ctgctttatg
    21061 gcaacaggca cgcagcccga tgctgggcag atcctcaact cgctgattaa cagtattttg
    21121 ttaatcgatg acaacctggc gatccattac gccaaccccg ccgcgcaaca actgctcgcc
    21181 caaagctccc gcaaattgtt tggtacaccg ttaccggaac tgttgagcta cttctcatta
    21241 aatatcgagc tgatgcaaga aagtctggag gcggggcaag gttttactga taacgaagtg
    21301 acgctggtca tcgacgggcg ctcgcatatc ctttctgtga cggcccagcg tatgccggac
    21361 ggcatgatcc tgctggagat ggcaccgatg gataaccagc gccgcttaag tcaggaacag
    21421 ctacagcacg cccagcaggt tgctgcccgt gatttagtgc gcggcctggc acatgagatt
    21481 aaaaatccgc ttggcggttt acgtggcgcg gcgcagctgc tcagcaaagc attacctgac
    21541 ccgtcactac tcgaatatac caaagtgatt atcgaacagg cggaccggct gcgaaatctg
    21601 gtcgaccgtc tgttgggacc gcaactgccc ggtacgcgcg ttaccgaaag tattcataaa
    21661 gtagctgaac gcgtggtgac gctggtgtcg atggaactgc cggacaacgt gcggttgatt
    21721 cgggattacg accccagcct gccggaactg gcgcacgacc cggatcaaat tgaacaggtc
    21781 ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc cggagggtgg tgaaatcatt
    21841 ctgcgtaccc gcacagcgtt tcaactgacc ttacacggcg agcgctatcg gctggcggcg
    21901 cgaattgatg tggaagataa cgggccaggt attccgcctc atttgcagga tacgctgttt
    21961 tacccgatgg tcagcggccg cgaaggtggc accgggcttg gcttatccat cgcccgtaat
    22021 ttgattgatc agcattcagg caaaattgaa tttaccagtt ggccaggtca taccgagttc
    22081 tcggtttacc tgcctatcag gaaataaagg tgacgtttat gcaacgaggg atagtctggg
    22141 tagtcgatga cgatagttcc atccgttggg tgcttgaacg tgcgctcgct ggagcgggtt
    22201 taacctgtac aacatttgag aacggcgcgg aggtactgga ggcgctggcg agcaaaacgc
    22261 cggatgtgct gctttcagat atccgtatgc cgggaatgga cgggctggcg ctgctcaagc
    22321 agattaaaca gcgccatccg atgcttccgg tcatcattat gaccgcacat tccgatctgg
    22381 atgctgccgt cagcgcctat caacaagggg catttgatta tctgcccaaa ccgtttgata
    22441 tcgacgaagc cgtggcgctg gttgagcgcg ccatcagtca ttaccaggaa cagcagcagc
    22501 cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat cggtgaagcg ccagccatgc
    22561 aggacgtgtt ccggattatc ggtcggcttt cgcgttcttc tattagcgtg ctgattaacg
    22621 gcgagtccgg caccggtaaa gaactggtcg ctcatgccct gcatcgccac agtccgcgag
    22681 ccaaagcgcc gtttatcgcg ctcaatatgg cggctatccc gaaggatttg atcgaatccg
    22741 aactgtttgg tcacgagaaa ggcgcattta ccggcgcgaa taccattcgt caggggcgtt
    22801 ttgaacaggc tgatggtggc acattattcc tcgatgaaat tggcgatatg ccgctggatg
    22861 tgcagacgcg tttgctgcgc gtactggcag atggtcagtt ttatcgcgtt ggcggctatg
    22921 cgccggtgaa agtagatgtg cggattatcg ctgccactca ccagaatctt gaacagcggg
    22981 tgcaggaagg taagttccgt gaggatctgt tccaccgcct gaacgtcatc cgcgttcatc
    23041 tgccgccact gcgtgagcgt cgggaagata ttccccgtct ggcacgccat tttttacagg
    23101 ttgccgcgcg cgaactgggc gtagaagcga agttgctgca tccggaaacc gaagccgcgc
    23161 tgacgcgcct ggcgtggccg gggaacgtgc gccagctgga aaacacctgt cgctggctaa
    23221 cggtgatggc cgccgggcag gaagtgttga ttcaggattt gcccggtgaa ctgtttgaat
    23281 caacggttgc ggagagtact tctcaaatgc aaccggacag ttgggcgaca cttttagcgc
    23341 agtgggcaga cagagcgctg cgttccggtc atcaaaatct gctttccgaa gcgcagcctg
    23401 agctggagcg gacgttactg acgaccgcgt tgcgacatac gcaagggcat aaacaggaag
    23461 cggcgcggct actcggctgg gggcgtaaca ctctgacgcg gaagttaaaa gagctgggga
    23521 tggagtgatt cacggcttgt gtgtaaagat tgattattga gcgcaaattg ctggtgtttt
    23581 gcgctttact gttccgataa gttcagtatg atcttgcccg gaaaacgggg agagtcatta
    23641 tgctggaatc aataattaat ctggtatcga gtggcgcagt tgacagccac acaccgcaaa
    23701 ctgctgttgc tgccgtgctg tgtgccgcga tgattgggct gtttagttga gtttcagatt
    23761 gctgatgaag tgctctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    23821 aagcaatatt ttcaacagat tttgcaggcc tgataagcgt agcgcatcag gcaatcttgt
    23881 tctcagcaca ttgatggcgc tttcgttttc tactttgtaa acgaagcgcc attcacaatt
    23941 taaatcaccc gagagaactg ctgcatccgc gctttctggc gcagataggt atcaaagcac
    24001 atgcaaatgt tgcggatcag caagcgacct ttcgccgtca cctgtattcc cttctcatcc
    24061 acatccacca gcccatcttt tgctaacggg gcgagcagct tgagatcttc ggcaaagtaa
    24121 tcagcgaaga gcaaatccca ctgttgctca atgggagcgt aatccagacg gaagttgcag
    24181 atgagcgact taatcacatc gcggcgaata cagtcatcac gcgttagcgc aataccacgc
    24241 cacagcgcat tgccttgttc atccacttgc tgatagtact gcttcaactc tttctggttc
    24301 tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc ccatccccag cagatcggta
    24361 tcgccctgag tggtgtagcc ctggaagtta cgatgcagca cgccttcacg ctgggccacc
    24421 gccagctcgt catccggacg ggcaaagtga tccataccga taaactgata gcccgattgt
    24481 gtcaggaagg cgatggtttc ctgcaggata tcgagttttt gctgcggact cggcaggtca
    24541 gcatctttga ttttgcgctg agcagcaaaa atggtcggca gatgcgcgta gttaaagaca
    24601 ctcagacgat cggggttcag ctccgccaca cgtttcaggg taaaggcgaa actctccggc
    24661 gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg aggtaaagcc aatctcacgc
    24721 gcatggttaa gcagtgcaaa gatgaactct tcatcctgct cgcggttaac cagacgttgc
    24781 acttctttgt tgaagtcctg cacgcccatg ctcaggcgat taaagccttc ggcgcgtaaa
    24841 tgatcgagta catccagttc gatttcccgc ggatcgactt cgatcgagat ctccgcatcg
    24901 gcattgaact ggaagttttc gcgcagcagc ttcatcagac ggctgatttg cgctttattc
    24961 agatacgtcg gcgttccgcc gccccagtgc aattggctga cgtgacgccc ggcgaacagc
    25021 ggtgcacgat ggacgatttc ttgctccagc gcgtccagat actgatcggc cttgtgctgc
    25081 tggcgagtaa ctatcttatt gcaaccgcag aagtaacaaa gcttatggca gaacggaata
    25141 tgtacgtaga gagataatgg acgctcagga tagcgcgcca cggcttgtaa aaacgcctgt
    25201 tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg aggtgtatcg tggcccggaa
    25261 tagttatatt tctggatcag ggccagatcc cagtcgattt gctgtacaga catgctcact
    25321 ccttccgatg gcgtctctgg cggctacggc gtaccggcgg atgcccgttt cgagtcatca
    25381 gccggttgcg cagccacttt tgtcgccgcg acaaccgctg tagtttaacg aataaccaca
    25441 ccagataaca tataaccgga agggttatca gcagaacggg aaggcccacg gcggcaatcc
    25501 gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt ctcgtcttct tcctcttctt
    25561 cgtcatcgtc ataagagagg ccgagtttct gcatcaactc atcaatacga tccagtttgg
    25621 catccaccca ggattgctct tcggcgctca gggtttcgcc tgcttccaga cgttccagca
    25681 gcgcatccag acgctcatcc gtttccagta actccaactc cgcctgcggt gaaagcatag
    25741 gtttctcact cttcggtttg tgctgtttgg tgactttttc agtcacgccc aatggaatag
    25801 gggttttact gccaatacgt ggatctttcg gtgcgttctg gcctttgctg cctgacgtgg
    25861 tgttaccgcc cgctgcgcgg ctgcccggcg catgaccacg acgttttttc tgacgcttac
    25921 ggtcacgagc ttcctgatcc agctcctcgc gtgtttttcg acgcgctttt gcatgacctt
    25981 tgctgcgtga gtttgaagat gatggtttca taatggttcg tcttttagcc gttttattca
    26041 gtatagattt gcggcggaat ctaacagaaa gcaagcaaag aaaaaaggcg acagattact
    26101 ctgtcgcctt ttttcctgac tcataaccct taacgggtct tacaatcctt gcaggccgcc
    26161 aacacaccct gtttttccat tagccggaac aatccgtgtt gattccattt ccgtttaatt
    26221 acgtctccgg acgtttatct tcctgacaat cctctgtctt cgcctcctgg cgctcctgac
    26281 cctcatcctg agtcattgtt cctgttagcg tcctcgctct atgggtgcta ctttacgcct
    26341 ttgctttaaa taaacaatca accgccatca gtatttgcac attttcagag ttttatataa
    26401 aagcaattca ctgatttata tagcttattt gacatacgtc gcagaatttt ctctgctatt
    26461 tcgcataaag actatcggca atgtctcaca aagcatatgg cttttactta caacgctcac
    26521 gccgaaaaat ccgcctttta ttatggttca ggtataatcg ccgcaaagct ttgactgatg
    26581 gagacgaccg ctttgactaa tttgaattat caacagacgc attttgtgat gagtgcgcct
    26641 gatattcgcc acctaccttc cgataccgga attgaagtgg cttttgcagg ccgttccaac
    26701 gcaggtaaat ccagcgcgct gaacacgctg actaaccaga aaagcctggc tcgtacctca
    26761 aaaaccccgg ggcgcaccca gcttatcaac ctgtttgaag tggctgacgg caagcgtctg
    26821 gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag agatgaagcg caaatggcag
    26881 cgcgcgctcg gcgagtacct cgaaaaacgt cagagcctgc aaggtctggt ggtgctaatg
    26941 gatattcgcc atccgctgaa agatttggat cagcagatga ttgagtgggc ggtagacagc
    27001 aatatcgccg tgctggtact gctgaccaaa gcggacaaac tggcaagcgg cgcacgtaaa
    27061 gcgcaattga atatggtgcg tgaagcggtg ctggcgttta acggtgatgt gcaggttgaa
    27121 acgttttctt cgctgaagaa acaaggcgtg gacaagctgc ggcagaaact ggatacctgg
    27181 tttagcgaga tgcagcctgt agaagaaacg caggacggcg aataattttc ttgccttaat
    27241 gcttgtgccg gatgtggcgt atcaggcatt acggatcttt tctttcgccc aataaaaaac
    27301 gccccagtca ttactgactg gggcggctaa aatattcagc caaatccgat tacgtgaagt
    27361 aaaaggtctg aaagatagaa catcttacct ctgtacccta cgcgactaac tttactcttt
    27421 tttgttcagt tcagaaagca ttttttgtaa ttttttttca gttgttgcat aggaatttct
    27481 aatagccatc acaaaacgtg acagcttatg ttgcttactt acgaaaaaag ggcatgttca
    27541 ggcgaatctt agtgcgcctg atcccagttt tcgccactcc ccacttccac cagcagcggc
    27601 acatccagac gggtacagtt ttccatcagt tgatgaatct gcttcgcgac ggcatcgaca
    27661 tcatctttat gaacttcaaa taccagttca tcgtgtacct gcatgatcat acgcacacgc
    27721 ggttgctcag cctgtagcca cgcatcaacg gcaatcatcg cccgtttgat aatgtcggcg
    27781 gcggttccct gcattggcgc gttaatggct gcacgttcag ccgctgcacg acgagcccca
    27841 ttgctggatt tgatatccgg cagatacaga cggcgtccgt ccagcgtttc aacgtagccc
    27901 tgctctttcg cctgagcacg ggtgcgttcc atatactcca gcacgccagg gtagcgttcg
    27961 aagtaaaggt ccatgtactt ctgcgcttct ttacgtggaa tgttcaattg ccgcgccaga
    28021 ccgaaagcac tcatgccata aatcagacca aagttgatcg ctttcgcgct acggcgttgc
    28081 tcgctggtta cggtttccag tggcaaacca aacacttctg ccgccgtagc ccggtggata
    28141 tcttttcctt ccgcgaatgc ggtcagcaag cctttgtcac gcgagagatg cgccataatg
    28201 cgcagttcaa tctgcgagta gtccgccgag acaatcacat aatcctctgg cgcaataaac
    28261 gcctggcgga tacgacgacc ttcttcgtta cgcaccggaa tgttttgcag gttaggatcg
    28321 gttgacgata aacgtcccgt tgcagttact gcctggtgat aagaggtatg cacacgcccg
    28381 gttttcgggt tgatcatcag cggcagcttg tcggtgtaag tcgatttcag cttcgccaga
    28441 ccacgatact ccagaatcac ttttggcaac ggatagtcca gcgccagttc ttccagtacc
    28501 tcttccgacg ttgacggcgc gccacccggc gttttcttca gcggtttaat tccctgtttt
    28561 tcaaacagaa tggtttgcaa ctgcttggtg gaggaaaggt taaattcttc acctgcaatt
    28621 tcatgcgctt tcttttccag ctcagccaga cgaagagtga gctcttcaga atgattgtgc
    28681 agcactttcg gatcgatctt cacaccgtta cgttcaatgc gtgaaagcac cggcactagc
    28741 ggcatttcga tattctcgaa gacgttcaac ggccctttgt gtttttgcag atccggccac
    28801 attttcagat gcaactgcaa ggtgacatct gcatcttcgg cggcgtaacg tccggcctct
    28861 tcgagggcaa tctggttaaa ggtcagttga tttttgcctt tacccgcaat ctcttcaaaa
    28921 gtgatggttt tgtgcttcaa ccaacgttcc gcgaggctgt ccatatcgtg acgcccggca
    28981 acgctattga gaatgtagga ctccagcatg gtatcaaacg caatcccacg cagctcaatg
    29041 ccgtaattcg ccagaatacc gcgatcgtat ttcaggtttt gcccaacctt cagcgccttt
    29101 tcatcttcca gcagcggttt tagcaactcg agtgcacgct cgcgagagat ttgatcgggc
    29161 gcatcaagat aatcatgagc aaccggaata tatgccgcta cgcctggctc gatagcaaaa
    29221 gaaagcccga ccaggttagc agagatgtta tcaaggctgt cggtttcggt atcaaatgca
    29281 aataccggcg ctttttccag cttcgcaatc cacgctttca gtgtttcttc atcaaggatg
    29341 gtgacgtagt tgtcataaga aatcaccgtt gccgtcactt ctggcgcttc gtctacaaca
    29401 ctggtttcct gcggcttcgc ggctggtttt gcccctttgg cctgtaacca tttgcccgct
    29461 tcgacatcag cagtccagcg tttgaactca tactttttga acagccctaa caactcttct
    29521 gccaccggtg gctgcacttc cagttgttca caggtcagct ccagttcaac gtcggtttta
    29581 atcgtcgcca gctggtaaga gagataagcc acttctttgt tttgctcgag cttcgccgcc
    29641 attgttttcg cgccacggaa gctcaaccca gcaatttttt ctggctcggc atacagcgta
    29701 tccagtccgc caagaccttg tagcaatgct tgcgcggttt tttcaccgac gcccggtacg
    29761 ccagggatgt tatcagagga gtcacccatc agcgccagga agtcgatgat cagttctggc
    29821 ggcacgccgt acttattcac cacctcttcc gggccgagga tggtattcgt catggtattg
    29881 ataagcgtaa tatttggcgt caccagctgc gccatatctt tatcgcctgt gcttatcagc
    29941 accggacgcc cggctttttc ggcttcccgc gccagagtac cgataacgtc gtccgcttct
    30001 acgccagaaa ccgccagcag cggcagtccc atcgctttaa ccatcgcgtg caagggttcg
    30061 atttgtgcac gcagatcgtc cggcattggc gggcgatgtg atttgtaatg ttcaaacagt
    30121 tcatcacgaa aggtttttcc cttggcgtca aagaccaccg ctgcatgcgt cggtttatat
    30181 tgcatgatta gactgcgcag catgttgagg acaccataca tcgcaccggt cggctcgcct
    30241 gcgctgttag tcagcggggg aaacgcgtga tatgcgcgat aaagataaga tgaaccatct
    30301 acaaggataa gtggattttg ggggatctga accataatgt ccgtgcctgt ttatcagatt
    30361 atgggtaaag gatgccacag acaggatgaa aacatgagtt tttcaccgct tttcgtgaaa
    30421 atcttttgta gatcttctgg atcgctcgca aatcgtcatg tggataactt tgtgcataaa
    30481 ttatttacat cgcttttaat ttattatcat gctttttaca gcatgatatt tgtctttgct
    30541 tttcatacag ttaaatataa agccatccct aataattgtg ataatgggat tttttctaat
    30601 gtggatatat atgaattatt tttctcctta aggatcatcc gttatttggg gcgttttctg
    30661 ctaaaatcgg cgccttgcgc ttctttagcg cactgtctat ggctaacctt ttgaataaat
    30721 tcattatgac gagaatactc gctgcgataa cccttttgct gagtatcgta ttgaccattt
    30781 tggtcactat tttttgctcc gtcccgatca tcattgccgg gattgtaaaa ctattgctac
    30841 ctgtgccagt catttggcga aaggtttcgc gtttttgtga ttttatgatg tattgctggt
    30901 gtgaaagtct ggcggtatta ctgcatctga acccacacct gcaatgggaa gttcacgggc
    30961 tggaagggtt aagtaagaag aactggtatc tgcttatttg taaccaccgt agttgggcag
    31021 atattgtcgt actgtgcgtg ctgtttcgta agcacattcc gatgaataaa tatttcctca
    31081 agcagcagct tgcctgggtg ccatttcttg gcctggcatg ctgggcgctg gatatgccgt
    31141 ttatgaagcg ttattcccgc gcttatttat tacgtcatcc ggaacgacgt ggtaaagatg
    31201 ttgaaaccac ccggcgttct tgtgaaaagt ttcgcctgca ccccaccacc attgtcaatt
    31261 tcgttgaagg ctcccgtttc acgcaagaaa aacatcagca aacgcactcc acttttcaaa
    31321 acttgttgcc accaaaggcg gcaggcattg cgatggcgct aaatgtactg ggtaaacaat
    31381 tcgataaact gcttaatgtt acgctgtgtt atccggacaa taaccgtcag ccgttcttcg
    31441 atatgttaag cggtaaactg acgcgaattg tcgtacatgt ggatttgcag cctatttccg
    31501 atgagttaca tggcgactac atcaatgaca aaagctttaa gcgccatttt cagcaatggc
    31561 tgaactcact gtggcaggaa aaagaccggc ttctgacgtc gttgatgtca tcacagcggc
    31621 aggacaagta aattttgctc gcctctgaat gagcagaggc gagtgagtat tttaatgaac
    31681 aaaacgtccg gcacgagaca taaattcttc ttcgctcatc tcctgtccgt taaaaacaac
    31741 tttacccggt gtataacgca gttgcagcga ggcggtattg ttgtcaatcg tgattaactg
    31801 aaacatctga cctaatgtca tcatcccgct gatttgttta tcagcccgct tttgcgcttt
    31861 ttcagcatcc attccttcag ataaattaag ctgttttgcg gtttctgtga caacattaag
    31921 cggtaacttc acatcaaaaa tgagcgattt aatatctttg tttgtggatg atgaggattt
    31981 ggctgggtcg gcaatactga tatccagatt ggcatttagt tcgccgagtg cattcttcca
    32041 tcttaccggt tgtttaatgg tcggctcact tttttgtaat aacggtaaat actctttgaa
    32101 caatgcagca ttcacttctt gcagagcaac ttcatcgttt gctaactcag ggtgtgcagc
    32161 aagctgcttc tgcatcgcaa tgttatactg gatgacaaat tggcgcaccg ctgagggatc
    32221 aatagattca gcaatcaatg accactcacc actaccgagt tgctgattcc cttttgttaa
    32281 cttatcaagt tcgtaggtca gattgacatt gatataatct ttgtctttaa cgcgatccag
    32341 cctggttttt gcaacgaaag catcgatttg ggcaacgtct tctccctgat tgataatgtt
    32401 aatctgattg aatttcgact cgctttcgcc aaccggaaat ttcttttctt ctaatcgcgt
    32461 tagagaatca atggtaaaac ttttcgccgt tactttattc aatggcgata attccagaac
    32521 aagatcttcg actttgcctt caatatccag attagacaat gaactaccat caccattaaa
    32581 agtaaactgg ccctcaccaa aagaaaactt tccatatttt gcaggggcaa catctaatgt
    32641 cgtggagaat gagccaccaa acgcgaatcg caatgtcgct tccataggag atttattttt
    32701 tgcggcgata aatagcggtt gtgtcagttc attatccact aatttcactt ttgccgctgc
    32761 cagtgcgggg atgacattac cacgcattaa cattgtgatg ggtaacggac cgtgctcaac
    32821 atccacatca aacgcaactt tctggccagg tttgatattg agatcaggtg caccgttatc
    32881 aaatgtcatt tgcatttgaa aacgagaatt aaaaaagcct ttgtcaaaat ttttataact
    32941 taacgtcata tcgtaggcat gttcaccttt ctttttagca tcgttaaaat ctttaataaa
    33001 cttttcgata ccaggctgaa tttgcgtacc tgtgtaccat gtgccaccac cccagattac
    33061 ggctaacgca acaataacac ctgtagctga cttacgtatc atataaatat tccctgataa
    33121 aacagcaaaa ctttgaatat ccacttatgc tgaaataatg tccagcggca g
//
LOCUS       DAZGAP010000029        33082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGAP010000029 DAZGAP010000000
VERSION     DAZGAP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.029"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..410)
                     /gene="yhaH"
                     /locus_tag="ACHLAM_001174"
     CDS             complement(45..410)
                     /gene="yhaH"
                     /locus_tag="ACHLAM_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7852738.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(701..1687)
                     /gene="yqjG"
                     /locus_tag="ACHLAM_001175"
     CDS             complement(701..1687)
                     /gene="yqjG"
                     /locus_tag="ACHLAM_001175"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO7852739.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1757..2149)
                     /gene="yqjF"
                     /locus_tag="ACHLAM_001176"
     CDS             complement(1757..2149)
                     /gene="yqjF"
                     /locus_tag="ACHLAM_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7852740.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2336..2635)
                     /gene="yqjK"
                     /locus_tag="ACHLAM_001177"
     CDS             complement(2336..2635)
                     /gene="yqjK"
                     /locus_tag="ACHLAM_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO7852741.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2625..3029)
                     /gene="yqjE"
                     /locus_tag="ACHLAM_001178"
     CDS             complement(2625..3029)
                     /gene="yqjE"
                     /locus_tag="ACHLAM_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7852742.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3032..3337)
                     /gene="yqjD"
                     /locus_tag="ACHLAM_001179"
     CDS             complement(3032..3337)
                     /gene="yqjD"
                     /locus_tag="ACHLAM_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO7852743.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3375..3743)
                     /gene="yqjC"
                     /locus_tag="ACHLAM_001180"
     CDS             complement(3375..3743)
                     /gene="yqjC"
                     /locus_tag="ACHLAM_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO7852744.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3890..4273)
                     /gene="mzrA"
                     /locus_tag="ACHLAM_001181"
     CDS             complement(3890..4273)
                     /gene="mzrA"
                     /locus_tag="ACHLAM_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO7852745.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4277..4939)
                     /gene="yqjA"
                     /locus_tag="ACHLAM_001182"
     CDS             complement(4277..4939)
                     /gene="yqjA"
                     /locus_tag="ACHLAM_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO7852746.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4961..5044)
                     /locus_tag="ACHLAM_001183"
     CDS             complement(4961..5044)
                     /locus_tag="ACHLAM_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852747.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            complement(5284..6060)
                     /gene="exuR"
                     /locus_tag="ACHLAM_001184"
     CDS             complement(5284..6060)
                     /gene="exuR"
                     /locus_tag="ACHLAM_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO7852748.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6190..7488)
                     /gene="exuT"
                     /locus_tag="ACHLAM_001185"
     CDS             complement(6190..7488)
                     /gene="exuT"
                     /locus_tag="ACHLAM_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO7852749.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            7971..9383
                     /gene="uxaC"
                     /locus_tag="ACHLAM_001186"
     CDS             7971..9383
                     /gene="uxaC"
                     /locus_tag="ACHLAM_001186"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO7852750.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9398..10885
                     /gene="uxaA"
                     /locus_tag="ACHLAM_001187"
     CDS             9398..10885
                     /gene="uxaA"
                     /locus_tag="ACHLAM_001187"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO7852751.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10968..11519
                     /gene="ygjV"
                     /locus_tag="ACHLAM_001188"
     CDS             10968..11519
                     /gene="ygjV"
                     /locus_tag="ACHLAM_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO7852752.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11524..12768)
                     /gene="sstT"
                     /locus_tag="ACHLAM_001189"
     CDS             complement(11524..12768)
                     /gene="sstT"
                     /locus_tag="ACHLAM_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO7852753.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLINDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12973..13275)
                     /locus_tag="ACHLAM_001190"
     CDS             complement(12973..13275)
                     /locus_tag="ACHLAM_001190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852754.1"
                     /translation="MASKSPKRSLEAGATRMDARRGRILQGRSLAPDPVAARDKLREP
                     RSGDFVRQSLGVQGAAAIGRPLRAPCASGFIWHSTLKERNRSHIPNSFAHMLSNQP"
     gene            complement(13384..14349)
                     /gene="alx"
                     /locus_tag="ACHLAM_001191"
     CDS             complement(13384..14349)
                     /gene="alx"
                     /locus_tag="ACHLAM_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO7852755.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14632..15618)
                     /gene="ygjR"
                     /locus_tag="ACHLAM_001192"
     CDS             complement(14632..15618)
                     /gene="ygjR"
                     /locus_tag="ACHLAM_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7852756.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15697..16380)
                     /gene="ygjQ"
                     /locus_tag="ACHLAM_001193"
     CDS             complement(15697..16380)
                     /gene="ygjQ"
                     /locus_tag="ACHLAM_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO7852757.1"
                     /translation="MQARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVGAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            complement(16457..16960)
                     /gene="ygjP"
                     /locus_tag="ACHLAM_001194"
     CDS             complement(16457..16960)
                     /gene="ygjP"
                     /locus_tag="ACHLAM_001194"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO7852758.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17045..18181
                     /gene="rlmG"
                     /locus_tag="ACHLAM_001195"
     CDS             17045..18181
                     /gene="rlmG"
                     /locus_tag="ACHLAM_001195"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO7852759.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18372..18455
                     /locus_tag="ACHLAM_001196"
                     /pseudo
     CDS             18372..18455
                     /locus_tag="ACHLAM_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            18452..18868
                     /gene="higA"
                     /locus_tag="ACHLAM_001197"
     CDS             18452..18868
                     /gene="higA"
                     /locus_tag="ACHLAM_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO7852760.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18913..20931)
                     /gene="fadH"
                     /locus_tag="ACHLAM_001198"
     CDS             complement(18913..20931)
                     /gene="fadH"
                     /locus_tag="ACHLAM_001198"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO7852761.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQQLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21102..23453)
                     /gene="ygjK"
                     /locus_tag="ACHLAM_001199"
     CDS             complement(21102..23453)
                     /gene="ygjK"
                     /locus_tag="ACHLAM_001199"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO7852762.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNKRNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKAETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(23470..24540)
                     /gene="ygjJ"
                     /locus_tag="ACHLAM_001200"
     CDS             complement(23470..24540)
                     /gene="ygjJ"
                     /locus_tag="ACHLAM_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO7852763.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            complement(24674..26107)
                     /gene="ygjI"
                     /locus_tag="ACHLAM_001201"
     CDS             complement(24674..26107)
                     /gene="ygjI"
                     /locus_tag="ACHLAM_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7852764.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(26170..26619)
                     /gene="ebgC"
                     /locus_tag="ACHLAM_001202"
     CDS             complement(26170..26619)
                     /gene="ebgC"
                     /locus_tag="ACHLAM_001202"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO7852765.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(26616..29708)
                     /gene="ebgA"
                     /locus_tag="ACHLAM_001203"
     CDS             complement(26616..29708)
                     /gene="ebgA"
                     /locus_tag="ACHLAM_001203"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO7852766.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENNLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(29892..30875)
                     /gene="ebgR"
                     /locus_tag="ACHLAM_001204"
     CDS             complement(29892..30875)
                     /gene="ebgR"
                     /locus_tag="ACHLAM_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO7852767.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            31094..31426
                     /gene="ygjH"
                     /locus_tag="ACHLAM_001205"
     CDS             31094..31426
                     /gene="ygjH"
                     /locus_tag="ACHLAM_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO7852768.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(31468..32847)
                     /gene="ygjG"
                     /locus_tag="ACHLAM_001206"
     CDS             complement(31468..32847)
                     /gene="ygjG"
                     /locus_tag="ACHLAM_001206"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO7852769.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
BASE COUNT         8054 a         8710 c         8557 g         7761 t
ORIGIN      
        1 tgataaaagc cggatgagtc agacatccgg cgagtagatt tgttttaagg ttccagtttt
       61 ggatcaggtc caaagcggtt ttcgcctggc gtacccgcct ggcagttaaa gacgatgata
      121 ataagccagc cgataaacgg gattaagaac agcagtgccc accacgccga gcggtcggtg
      181 tcgtgcaggc ggcggaactg aactgcccac cacggtaaaa acaccagaat accgtagatt
      241 gtcgtcagga tgccttcgcc accggcacgt tgccagccta acattttatc cagcagcccc
      301 agcacgaacg taaagatgat gttgaccaga ataaacatcc agtactcttt gcgccgcgca
      361 cgaccccgga aaccgacata attttttagt actttcagat accagtccat ttttgcctca
      421 attttgctca atcacttgtt tttaaagcag caaattaaga atagacgtga atgaaatggc
      481 agtaaatatt cgttcaagga attaataaag tgccgtaaag cgcgctaaga ctgatgacaa
      541 gcgcaaaatc gccagatgcg ctacgcttat ctggcctacg cggcattgta acatattgaa
      601 tataaacgtt ttgtaggttg gagaagacgt tcacgccgca tccgacataa ataacacgca
      661 cttagctaat aatcagaggc acctcgtaag gtgccttttt ttaaccgaag cgaacatctc
      721 gtccatgcgg ttcatcgaga tcctgccacg gaccaattga aataatcccc gtagggttga
      781 tggtcttatg gctgcggaag taatgattac ggatgtgatt gaaattgaca gtttcggcga
      841 tccccggcat ctggtagata tcgcgcagaa aaccgtacag attcaggtaa tcgctgatgc
      901 ggtgcttatc acacttgaag tgggtcacat acaccggatc aaaacggacc agcgtggtcc
      961 acaggcgaat atcggcttcg gttagctgat tgccggtcag gtaacgatgc tggcctaaaa
     1021 tctgctccag tcgcgccagc gattcaaaca ctttcgccac cgcctcgtcg taagcttgct
     1081 ggctggtggc aaaaccagct ttatacacgc cgttgttaac ggtgtcataa atccagccgt
     1141 taagttcgtc aattttcgtt tgcagggctg gtgggtagta atcacccgct ttcgcgccca
     1201 gcgcatcaaa cgcggtattg aacatgcgga tgatttccgc tgattcgttg ctgacgatgg
     1261 tgtggttctt tttgtcccac agtacgggaa cagtaactcg tccgctgtag tgtggatcgg
     1321 cgtggagata aagctgatac aaaaattcat gttgatagag tgtatcgccg gttgcacccg
     1381 gaaaactgtc atcaaaggtc cagccgtttt ccagcatcag cgggttcact acagaaacgg
     1441 aaataaacgg ttccagtcct ttgagtttgc gcatgatcag cgtgcggtgc gcccacgggc
     1501 aggcgagtga aacatagaga tgataacgat ctttctctgc gataaaaccg cctgtgccag
     1561 tggggcctgg cgcgccatcg gcagtgagcc agttacgaaa tgcggaagct gaacgttgaa
     1621 atttaccgcc ggtagatttg gtatcgtacc aggtgtcatg ccagacgccg tcaatcagtt
     1681 gacccatatt tctctcctcc aatagcaaaa acgaggagct tcctcctcgt tttttatcgt
     1741 taagtatagc gcgtgcttac cactttttat tcagcaggcg gtcgatgcta tacgcgcccg
     1801 gaccggtaat tgccagcagc aggaatccgc cagaaattgt caggtttttc atgaacatca
     1861 gcgagttgac gccttcagca aagttgctgt gaaataaaaa tgccgtcagc agcgtaaagc
     1921 ccgcagtaaa cagggctgtg gtgcgagtca ggaaaccgaa caggattgcc agaccaccac
     1981 caaactcaag cagaatcacc agcggcagca taaaacccgg gacgcccatc gcttccatat
     2041 attgttgggt acccgcgtaa ccagtaattt ttccccagcc tgcggtaata aacagaatcg
     2101 gcattaaaat gcgcgctacc agtacaccaa catcttctaa ttttttcatc atactctcca
     2161 ctgcgccgct caggctgctg attgtttttt tgtgcaaggg cgcgctatta gcgtcgttgc
     2221 tgtcgatgga gagaatcata aacgtggcga agcgtgattg ttagcaagga aaactgtcaa
     2281 atattttcaa aaaatttgag ggataaggcc ggaatttctc cggccagaga ggaagttaac
     2341 cgcgaagctg ttgttgcttg agggttgttt tcaccagacg ccaggcgctc catacaccaa
     2401 aaccgcgtct ggcccagcgg accagcatat taggatggcg aatcgtccag atagccatca
     2461 cgctactgcc aaccagcgcc caggagcgca gactcaacag catattccag cgacgatcgt
     2521 aagcgcctgt tgcctccagc cattcacgac gactggcgga aagatccagc cgttgttgct
     2581 ggatctggct aagcagttgc gccttacgtc gttcacgttc gactttactg ctcacgggac
     2641 tcctcctcga gcagctgccg atcgtttgcc agctcatggc gcgtgtggcg cagcaacgta
     2701 gacttacgcg atttacgtaa cgtccagatg ccgccaatca gtgccagtag cagcaacacc
     2761 acggtggtgg caatcatcgc attcaggcga tattgcggat caaccgccca gataattagc
     2821 accatcaggc tcataagacc aaatgcagcg aaaagcatcg tcaggcccag catcagtaaa
     2881 agttgaaaga gattcgcttt ttcctcttcc agctccacca ccgccagccg cagacgtgtc
     2941 tccaccattt caaccatgat ggaaacaatt cgctgcccga tgcccagaac gcttttaccg
     3001 ggcccttgcg cgtgatgagt gtccgccata attaacgacg cgacagcaga acaccgagca
     3061 ctacaccgat tgctgcgcca atgcccacgc ccgtccacgg attttcacgc acatactcat
     3121 cggcacgcgc cgccgcgaca cgggtttgtt tggcaatggc atcaccggtt tcacccaggc
     3181 gataacggct ctgtttcagt gcctgctccg ctttgctacg aatcttactc aactcttctt
     3241 tcgacttctc gccagatgag ctaagcacct cttccagcgt atcggaaagg gatttcaact
     3301 cagcacgcag atgttccgta gtgtgttctt tcgacatagt tttctccagg tgagtaagta
     3361 gtgactattg tgagttagta gtcgcgcgct tccagctttt tcagctcttc ctgcgcttct
     3421 gccagtttcc gttcgcgttt ggcaatctta tctgcatcgc ctttttgctt cgcctcggct
     3481 aaatcttgct ggcgttccgc cacctcatct ttctgctttg cgattttctt ctgatgatcg
     3541 gcacgcagct ggctatctga acagttggcc cggacttcac tcagggcttt attcagaccg
     3601 tcaatacgat tctggttttg gtgtttttcg gcatagctga tctccttaag gatattttgc
     3661 tccttttcct gacacagggt agtggcataa ctaccggcac taagagcaaa gagagaaaca
     3721 gctaaagcga tgcggtattt cattcttgaa ccttccattg tgattcggac ctattacaaa
     3781 ggaacgattg tccagacaac gaagcggtcg tgttaaccac cgcctcatta atcatagaca
     3841 gttacggtga aatcaccaaa gtgagtgatc tgtttcggat aacaggaagt tatccgaagc
     3901 gatgagagtt atcccgcaac cgggtcagcc actgcatagc ctgactgtta ttgtcctgct
     3961 gcgcaatgat gtagccatgg ggcaatgttc tgtcgaggac cgcttttgcg gcggcgctct
     4021 ggtcgctgga gtcaaaggta attaacagag tgtcgttttt gggggtgata cttttgaaag
     4081 gaatgccgtg agcgtcaagg tgatgccaga ttgaaaaacc gtctggcatc gttgtccctt
     4141 gatgaacggc acgaatcgcc agcgtagact cttgctggcg aaccgcggac caggccagca
     4201 acagtgtgcc gaccagaagt aaaacagcgc cggaccaggc tagctggcga agcgacatgc
     4261 gaggtatttg catcccttac ccccgatttc catatttctt tttccataac acgaccagag
     4321 aacctgccag gccaaacacc agcagcacca ccgggagcag catcaggcat gacatcagct
     4381 ggtcctcgta ctttaaaaat accggcgttt tgccgagcat gtaacccaga gttgtcagga
     4441 tcaataccca caacaaacca ctcatccagt tgaagaactg aaagcgcgcg ttattcagcc
     4501 ctgataaccc ggcaatcgtc ggcagcagtg ttctgacaaa cgcaataaag cgaccaatta
     4561 acagcgccga taaaccgtgt ttatgaaaaa gatggtgtgc gcgttgatga taatgcgcgg
     4621 gtaaatgaga tagccagttt tgtacggtgc gggtattgcc cagccatcgc ccctgaatat
     4681 agctgaccca gcagccgagg ctggcggcaa cggtcagcag cagaatcgtt tgcggatagc
     4741 ccatcgcgcc tttcgcaatc aatacaccga ccaacaccag taagctgtcg cccggtaaaa
     4801 aggctgccgg aagcaagccg ttttcaagga acaaaatgac aaacaagaca aaatacaaca
     4861 tgccaatcat cgacggattg gccagggttt caaaatcctg cgcccacagg gcttgcagca
     4921 attgggtcaa aagttccatt cattattcct ggtgaatcgg ttaacgccac gctggtttta
     4981 aatgatgcac taccgcaatg ttattgctgt cttggtatgt tctgcgggtt tgatacatca
     5041 acacagcctg aactattccc tgttaaatcg tgagataaga aagcattcaa aagcataaat
     5101 tcaaattgaa tctaattgta acaaaggtca acgttgttcg ttatcaaaat tacatgtccg
     5161 tggagtctga ttttgctgaa tgcaggggag ggaggcgaga ggatttacac aggctgacac
     5221 tttatacttt tcgcttaccg acgagacaaa caggcgcact gtagcgcctg tcggacagga
     5281 atttcattta ctgccgctgg tggctgtatc gagatgcacc accgggtttt cggcgaacag
     5341 atagcggtcg gcattgaact cgaaatcgtc gctggtttcg ttaaataaca tgatcttggt
     5401 gttttccagg tgctgccaca ttgccagctt agcagcatga ggatctttgc gaatcagcgc
     5461 cttgaggatt tgatcgtggt catcacacca gttatcgacg gtacgggcat caatgtgttc
     5521 gtgcagtttt ttccagtacg ggttatgact acgctgggtc cacatttttt ccacgatagc
     5581 cgccagggcg gagttctgcg tcgccagggc tacctgaatg tggaactgca aatcccactc
     5641 ggaatcacgg aagcattgtt cgccgcgcgc ctgctcctgg atggccatca gtttcatgat
     5701 gtcctgtttc gtcacctgag ttgccgcgaa ctcggcaata ttactttcga tgagctggcg
     5761 agcctggagc aactcaaacg gaccgtaatt ggcgaattcc atattattgt cagtcgcctg
     5821 ctgatggcgc ggctggttgg aaaccacatg aatacctgaa cctttacgca cttcgacata
     5881 gccttcaact tccagcatga tgatggcttc acgaaccacg gtgcggctga cgttcttttc
     5941 atcggcaata aagcgttcag caggcagttt atcacccacc agatagacgc cctgttcgat
     6001 gcgctctttc agatcagcgg caagttgttg atacaaacgg cgtggttcag tgatttccat
     6061 atgcgctcca ggcatgatac ggcagactat atttgttata ccacttttgc gagtttagct
     6121 ctactcacag tgatgaaaaa gccgcctgag aaggcggctt tgaagggaaa ctaaaactta
     6181 cacgaagcct taatgttgcg gtgcgggatc gttatgtgat tcctgcgcca cctcaatcgc
     6241 cggtttgttc tgcaacacgg tccagataac cagcgcacct aacaggtcga acactgccag
     6301 aactgcgaac agcgggctga agccgatggt gtcagccagt gcaccgacta ccagtgcaaa
     6361 cagcgtactt gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt
     6421 acgaccgaac acgtcagacg agagcgtaat cagtgcgcca gacagtgcct ggtgagcaaa
     6481 accaccgata cacagcagca taatcgcgac atacgggttg gtgaacagac cgatcatacc
     6541 cgggccaatc atcagcaccg cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt
     6601 cacaccaaac caacgctgga acagtggtgg caggtaacca ccgaggatac aaccgaggtc
     6661 agcaaacagc atcggcatcc aggcaaacat cgcgatctct ttcaggttaa agccgtaaac
     6721 tttaaacatg aacagcggga tccacgcgtt aaaagtaccc catgccggtt ctgccaggaa
     6781 gcgcggcagc gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt
     6841 cttcgctgtg tcaacctggt gctgggcttc ctgaccatta ataatgtagt cgcgttcttc
     6901 atcggtcaga tgtttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca
     6961 gataaagctc aacgcaccgg agatgataaa tgccatctgc cagctgtgca tcacgattgc
     7021 ccataccacc agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc
     7081 taccgcgatg gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg
     7141 aatcatcgcg gcttccgcgg caccgaccgc accacgagca acagccaggc cgccccagct
     7201 acctgccagc gcggttgcac cacagaacac ggcccacagc acagcaaaca ttgcataacc
     7261 gattttcgta cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc
     7321 agcagaatag gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat
     7381 cagagttggc gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccgag
     7441 cgtcaccagt gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat
     7501 cgttttgtta tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt
     7561 tacgaccggg taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg
     7621 ctaattcggc ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt
     7681 tgcattccgt cgacttatga tttgcgacgg cagaaagata acttgtcata caactttaaa
     7741 aggtgagagc catcacaaat gtgcgaatat ttgtagggac attacctgac gacggcgagg
     7801 ccagtactgg cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg
     7861 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     7921 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     7981 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     8041 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     8101 actatcgttt taaaaacctg tatgacatct ggttgaaggg cgatcactac aaatggcgcg
     8161 ctatgcgtac caacggtgtg gccgagcgtc tgtgcaccgg tgatgcgtct gaccgtgaaa
     8221 aatttgacgc ctgggcggcg actgttccgc acactattgg caacccgtta taccactgga
     8281 cgcacctcga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     8341 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tctgcgcgcg
     8401 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     8461 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     8521 ggcgtccgga caaagccttt aacatcgaac aggcgacctt taacgactac atggcgaagc
     8581 tgggtgaagt ctctgatacc gacatccgcc gctttgctga tctgcaaact gccctgacca
     8641 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccacgcg ctggatgtgg
     8701 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggcag
     8761 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtgctg gtgttccttg
     8821 gtgccgaata cgcacgtcgc ggctgggtac agcagtacca catcggcgcg ctgcgtaata
     8881 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaatgacc
     8941 gtccgatggc agaagagctg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     9001 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     9061 gtaacttcca gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     9121 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgttga
     9181 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     9241 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     9301 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     9361 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     9421 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     9481 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     9541 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     9601 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     9661 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     9721 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aatgagctgt ggatcctgcc
     9781 aaccgtgggc tgtgtcaacg gcatcgcgcg gcagatccaa aatcgtttcc tgaaagagac
     9841 caacaacgcc gaaggtactg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     9901 gctgggcgac gatcacatca atacccgcac catgctgcaa aacatggtgc gccacccgaa
     9961 cgcaggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
    10021 tgaaacgctg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
    10081 tgatgagatc gaagccggga tcgcgcattt gcatcagctg tataacgtga tgcgcaacga
    10141 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
    10201 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
    10261 taacggtggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
    10321 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
    10381 caagcagtac tttattgccc atgaccagcc gatctacgaa aacccatcac cggggaacaa
    10441 agcaggcggt atcaccacgc tggaagataa atcgcttggc tgtacccaga aagcgggttc
    10501 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccgg ggctgaactt
    10561 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
    10621 tatggtgctg ttcagtactg gtcgtggcac gccgtatggt ggatttgtgc cgacggtgaa
    10681 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
    10741 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaattcatcg ataccatcgt
    10801 tgagtttgcc aacggtaagc aaacctgtaa cgagcgtaac gacttccgcg aactggcgat
    10861 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
    10921 ataatgtgaa gcgccgtttg cttttccaga cctgctaagg atttatcatg accgcgtatt
    10981 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaacc
    11041 gtgacgaacg ccgctttaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
    11101 tttttcttct gggcacctat cccgccggtg ccagtgccat ccttaatgcc attcgtacgc
    11161 tgattacttt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
    11221 gaattggcct cgcgaagttc catcatcctg tcgaactact gccggtaatc ggcacgattg
    11281 tcagtacctg ggcgctgttc cgctgtaaag ggctgaccat gcgctgcgta atgtggtttt
    11341 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acgatgattg
    11401 aggggagttt tctgctgatg aatggtctga atatcattcg tttctggcgg atgcagaaaa
    11461 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc catagacgaa aggggttaaa
    11521 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
    11581 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
    11641 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
    11701 acaggccagt gggatcagca gcagagaccc ccccgccacg ccggatgcgc cacaggcaca
    11761 cagagaagcc actacgctca acagcagcgc tgtgggcaga tcgaccggaa tacccagcgt
    11821 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
    11881 ggctcccagc ggaatagaaa cagaataggt atcgcgatcc agattcagct tttcacacag
    11941 cgccatattc accggaatgt tcgctgcaga gctgcgggtg aagaaggcat acacaccgct
    12001 ttcacgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    12061 caatgggtta accaccagcg ccaccagcag catacagcca accagcacga ccagcagttg
    12121 cgcgtagccc cacagcgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    12181 aataccgatc ggtgcgaagc gaatcaccag tttcaccata aaggtgacgg cattcgacat
    12241 atcgttgatc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    12301 tgcccacacc aggatcccga tatagttacc ttttagcagc gcgtcaatgg ggttggaaac
    12361 catgctcatt accagcccgc gcattacttc tacaatgcct gacggcggcg aaatatcacc
    12421 cgcgctactg gacaagtgca gggtagaagg gaaggcaaaa ctgaagatta ctgcggccag
    12481 agcagcagag aaggtgccca gcagatagag gaacaaaata gggcggatat tggttttctg
    12541 cccgtgctgg tggttagcaa tagatgccat taccagcatc aacaccagga tgggggcaac
    12601 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    12661 tgagatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    12721 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    12781 tcattgtgtt gtcgcacatc cttggtgcgt tctgtcgacg catctttcag gatgtaacaa
    12841 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    12901 tttaaagaga ggtcatattt tgcaacttaa tggttacatt tatttgacat aaacagaggg
    12961 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    13021 acggttgcgc tcctttaatg tgctatgcca tataaagcca ctggcgcagg gagcgcgcag
    13081 ggggcggcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
    13141 gcgcggttcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
    13201 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
    13261 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctataa gccgattaca
    13321 taattaatag ctcacctttg tctggaatga gttatctcta gtaatggctt aatatacagc
    13381 gaatcattct ccccgctgct tatcatgccg ataattcacc caggcgttga taataaacgt
    13441 catcaccaga atgccaaaca ccacgcccag cgagacggcg atcggaatat ggtagaagtc
    13501 gacaatcagc atcttgatac cgataaacac cagaatcacc gccagaccat atttgagcat
    13561 cgagaaacgc tctgccacac ctgccagcag gaaatacatc gcacgcaggc cgaggatcgc
    13621 aaacaggttt gaggtcagca caataaacgg atcggtggtt acggcgaaga tagccggaat
    13681 actatccacg gcgaaaatca cgtcgctcaa ctccaccaga atcagcacca gcatcagcgg
    13741 cgtggcatac aacaaaccat ttttacgcac aaagaaatgc tcgttgtcga tggtgtcggt
    13801 catgcgcaaa tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc
    13861 ttcatgggcg agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag
    13921 gatccagtcg aactgtgaaa tcagccagct gccagtgaag atcatgatgg tacgcagaac
    13981 aatcgccccg agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa
    14041 atagctgaac aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag
    14101 ataacccgtg agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cggtttgcac
    14161 cagataccac cagaaagcgg cgttaaacag taacgacagc gtcacccaga ccagcgacca
    14221 ggccgcagcc tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc
    14281 gatagccagc ataatggtga caacaacagc gaatccgccc catagcaacg gcgtgccgac
    14341 agtattcata gaagttcctt acacataaaa aaacggccaa cgtcggaaga cgtcagccgc
    14401 tgctttttat gcatagacct cgccttccgg caaggtctca cttacaacaa aaaaggaata
    14461 cgtctgcgta ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat
    14521 gacgatcgac cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagaccatt
    14581 cagtcagtca atggtctgtt acataccttt tacactgttt tactttggca attatagttt
    14641 tacgctatct gccggaaaaa tcaccccagt ctggcggcgg atctcggtca gcagtttagc
    14701 ggtgatgcga ctgaccgcca gccctggatg atccaccaga tgctcatcca ccagttcagc
    14761 aaacagctct gcttcgtaga gcatggtatt aatatgctgc ggttgggtca gatcctgcat
    14821 ctggctgcca cgcggcacaa aacacacttt ctggcattca gacagttttt caatcaccag
    14881 cgatcccgct tcgccctgaa tctcgcttgc caggacagaa tcgctcactt ttgagtgctg
    14941 caacgtaacg ctgaaatcac cgtaatccat caccaccacg ccgtgggcat ccacaccgct
    15001 tgccagcaaa ctggcggttg cctgcacgtt tttcggttca ccgaataatg ccaccgccga
    15061 cgccagacag taaaagccga tatccataat tgaaccgtta gagaatgctg gattaaaggt
    15121 gttgggattc tcaccatcca ggtaacgttg ataccgcgag gaatactggc aatagttgaa
    15181 aaagacttta cgcagtttgc cgactttcgg cagcgcctgg cgcaacagat gaaagttcgg
    15241 caggtaggcc gttttaaatg cctcaaacag caccacctga ttttcccgcg cacaggcaat
    15301 ggcggcatcc acttccgcca gattcgatgc cagcggcttc tcgcaaatca catgaatttt
    15361 gtgactaagg aaaagttgtg tctgggaaaa gtgcagggca ttcgggctgg caatatacac
    15421 cgcgtcaatg gcatcgcttt ccgccatcgc ttccagcgac gtaaacagat gctcgacaga
    15481 aaaatcattg gcaaagtgct gggcctgttc aaggctgcgg gaatatacgg cggttaactt
    15541 gtatttaccg ctctcatggg cggcctcgac gaactggcga gtgatccagt tcgtaccaat
    15601 cacagcgaaa cgtatcataa cgcgtggata ctccataaag ggattctgcc gtcaatgtag
    15661 cacgcctttt tcatcttcga cgtgcgcaga tacgcattat ttctgcgccg gacagccatg
    15721 ctcactaaac ggcccaatca tgaccgatgg cccgagatat ttcggctggc gatggaggat
    15781 ttttgcgtca atcaccgcgc cgacgcgagc gagtttctcc cgcagttgag tataaaaacc
    15841 gtgcttcata ttgaggtctg gcgcgttaaa accgatagcg tcgatgccgt actgttttgc
    15901 cagccagatg gcgcgttggt tatgaaattc ttgcgaaata atggtgatat ggttttcccc
    15961 aaacaccttt ttggcacgca ccaccgaatc aagcgttgag aatccggcat agtcgcagaa
    16021 gatcactttc gccggtacgc ctttagcgat caaagcctgc tgcatacctg aggcttcatc
    16081 gtaattttta cgcccgttat cgccacttac cagtagccat ttcacttttc cggcgtggta
    16141 caacgctgct gcggtatcaa tacgtcgggt aaagtagcga ttacccggcc tcgcccccag
    16201 caataaaccc acgttacgcg ccggaactgc gctgacatcg ctccaggtca gctgcttgct
    16261 ggtgttgacc ataacgcgat cggcaataaa aatggtcgct cctgctataa gcaacaataa
    16321 acagccaatt ttaagagtcc ggcgagaaaa acaaatacgg agaagaaggc gggcctgcat
    16381 ggtcgttatc cttaacgaaa ggggggaggt cgattttagg cgctatagga agaaaagata
    16441 acgcctgaca tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc
    16501 gggtgtcgaa ctcaagctgg tgatactgcg gctccatatg gcagcacaat tgatagaacg
    16561 ctttgttatg ctctttctcc ttcaggtgcg ccaactcgtg caccacgatc atgcgcaaaa
    16621 acggttccgg cgcgttgcga aacacggtag cgacgcggat ctccgccttt gctttcagct
    16681 tgccgccctg cacacgcgaa acggcagtat gtaatccgag cgcgtttttc agcacgtgga
    16741 tcttattgtc atacatcact ttagtgatcg gcggggcatt gcgcagaaac tgatttttaa
    16801 gatcctgagt atattgccag agggctttat cggtggcgta gtcgtgtgtt cccggatagc
    16861 gttttgccag cacatctccc agacgctgtt cgttaatcag cgttcgcacc tgggaaagta
    16921 gctgctcggg ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga
    16981 aaaagggtat actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg
    17041 gccgatgagc cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac
    17101 ggatgacgtt aacccactac aggcgtggga agcggcggat gaatatttgc tgcaacagtt
    17161 ggacgacaca gaaatccgcg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag
    17221 ttgtgcgctg gcggaacata agccatacag cattggcgac tcatacatca gcgaactggc
    17281 gacgcgcgag aatttacgcc tcaacgggat tgatgagtcg agcgtgaagt ttctcgacag
    17341 caccgccgac tacccgcaac agccgggcgt ggtactgatc aaagtaccga aaacactggc
    17401 gttgctggaa caacaactgc gtgcgctgcg caaagtggtc acgccggata cacgtattat
    17461 tgccggtgcc aaagcccgtg acatccacac ttccacgctg gaactgttcg aaaaagtgct
    17521 cggtccgacc accaccacgc tggcatggaa gaaagcgcgc ctgattaact gcaccttcaa
    17581 cgaaccgccg ttggccgatg cgccgcagac cgttagctgg aagctggaag gcactgactg
    17641 gactatccac aaccatgcga atgttttctc ccgcactggg ctggatattg gcgcgcgctt
    17701 ctttatgcaa catctgccag agaatctcga aggtgagatt gtcgatctcg gttgcggtaa
    17761 tggcgttatt ggcctgacat tgctcgataa aaacccgcag gcgaaagtgg tgtttgtcga
    17821 tgaatcaccg atggcggttg cttccagccg tttgaacgtt gaaaccaaca tgcccgaggc
    17881 gttggatcgc tgcgagttta tgatcaacaa cgcgctctcc ggcgtggagc ctttccgttt
    17941 taatgctgtg ctctgcaacc cgccgtttca tcagcaacat gcgctgaccg ataacgtcgc
    18001 ctgggagatg ttccaccatg cccgccgctg cctgaaaatc aacggcgagc tgtatatcgt
    18061 tgccaaccgt cacctggatt acttccataa actgaagaaa attttcggca actgcaccac
    18121 catcgccacg aataataaat ttgtggtgct gaaagcagtg aagctggggc gtcgtcgata
    18181 agattgggag ttacctgccg gatgcggcgt aaacgcctta tccggcctac gttcagcaca
    18241 ttacccccag gtcggataag acgcggcaag cgtcgcatcc ggcaatgtct gcccagttct
    18301 ggaatcgcgc ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta
    18361 gcgtttttct acgtttatgc tacatacgtg aagttatgac gcataaagaa tacgatttct
    18421 ttaccgccgt tcatcgtact aaggggaaaa aatgattgcg attgccgaca tcttgcaagc
    18481 aggagaaaag ctaactgctg tggcaccttt tctggcgggt attcagaacg aggaacaata
    18541 cgcccaggcg ctggaactgg tcgatcatct gctgctcaac gatcctgaaa atcccttgct
    18601 ggatctggtg tgtgccaaaa taaccgcgtg ggaagaatca gcgcccgaat ttgctgaatt
    18661 taatgccatg gctcaagcca tgcctggcgg tatagccgtg attcgtaccc ttatggatca
    18721 atatggttta accctttccg atcttccgga aattggcagt aaatccatgg tgtcacgcgt
    18781 tttgagcggg aagaggaaat taacgctgga acacgctaaa aaactggcaa cgcgattcgg
    18841 catttctccc gccttgttta ttgattaacg cgacgtgccg gatgcgtcgc atccggcgct
    18901 tcaaacaacg atttaaatct ccagcgccag ccgtgttccc tgggcaattg cccgtcgtgc
    18961 gtcaagctcc atagctacat cgcagccgcc aattaaatgc acagttttcc cgctatcaat
    19021 cagctgttgc gccagcgcgc ggtttggctc ttgccctgcg cagatcacca cattgtccac
    19081 tgctaatacc tgcgtttcgc cgttgatcac cacatgcagc ccgtcatcgt caatcttctg
    19141 ataacttacg cctgggatca ttttcacacc ccgcgagagc agggtggtgc gatggatcca
    19201 gccggtggtt ttgcctaacc cctgtcccgg tttgctggct ttgcgctgga gcatcacaat
    19261 ctgccgtggg ctacgcggga tctgcattcc ctgcgggctt aagccaccag cctgttgcag
    19321 gctgctgtcg atcccccact cattacagaa ctcggcaatg ttctggctgg tggactcacc
    19381 cggctgactt aaaaacatcg ccgtatcaaa accaatcccg ccacaaccga tgattgctac
    19441 ctttttgccc acaggcgctt tgtcgcgcag tacgtcgaga taactcaaca ccttcggatg
    19501 atcgattccg tcgataggtg gaatgcgcgg tacgatcccg ctagcgagga tcgtttcatc
    19561 gaacgactgt aactgatccg ccgtcacggt gtgattgagt ttcagcgtca cgcctgttac
    19621 ttcgatcatc cggcggtaat agcgcagcgt ttcgtaaaac tcctctttgc cagggatctg
    19681 tttggcgata ttaaactgac cgccgatctc actgtgagca tcaaacaacg ttacctgatg
    19741 accacgtgcc gccgcgttaa tggcaaacgc cagcccagcc ggaccagcgc cgaccaccgc
    19801 cagatttttt ttctgcacgg cgggaaggat cggcattttg gtttcgtggc aggcgcgagg
    19861 attcaccagg cacgaggtga ctttgccaac gaagatctga tcgaggcagg cttgattgca
    19921 gccaatacag gtgttgatct catcggctcg ccccgattgc gcttttgaca gcagttccgc
    19981 atcagcaaga aacggtcgcg ccatcgatac catatcggca tcaccgcgcg agagaatgtc
    20041 gtcggcaacc tgcggatcgt taatccggtt ggtggtcacc agcggcagcg agacgtggct
    20101 tttcagtttg cgcgtgaccc agctaaaggc accgcgcggc acgggcgtgg caatggtcgg
    20161 aatacgtgct tcatgccagc caatgccggt gttgataatg gtcgcgcccg ccgcttcaat
    20221 ggcctgcgcc agccctaccg tttctgcaaa agtaccgccg ccttctacca ggtcgagcat
    20281 cgacagacgg tagataataa tgaagtcatt gccgacgcgt tcgcgcactg ctcgcaccac
    20341 ttcaactgca aagcgcatcc ggttgcggta atcgccgccc cactggtcgc tacgctggtt
    20401 ggtgcgcagg gtcagaaact cgttgatcaa atacccttcg gaacccatca cttctacgcc
    20461 gtcgtatccg gcctcccgcg ccagttgcgc gcagcgggcg aaattgtcga tcagttgcag
    20521 gatctcttca tggcttaact catggggcac gaaacggttg atgggggcct gcaatgcgga
    20581 gggagcgacc agatgcggtt ggtagctgta gcgcccggta tgcaaaattt gtaaggcaat
    20641 tttgccgcct tcctgatgta cggcttcggt aatggtgcga tggtgtggga tctggctggc
    20701 gtcgttgagc attgcgccgc cttccatgcc aacgcctgtt aaatctggtg caatgccgcc
    20761 gctgacaatc aacgccacgc catgacgggc gcgttccgcg taaaacgctg ccagccgttc
    20821 agcgccgtcc gggtattcct ccagtccggt gtgcattgaa cccatcagta ctcggttttt
    20881 taacgtggtg aagcctaaat ccagcggggc gaacagcgac gggtagctca taaaattgtc
    20941 cagtatgtaa aataattgtt atgtggtcgg atgagttcta atttagccgt cgctggagaa
    21001 aagggaaaag gggagtgcgg ggtttgtgat gggattcaaa aagaatacgc ccgttcgtca
    21061 tgccggacgg cggtaataca gtcgtccggc atcgggaaaa attactgctt acggaaaaaa
    21121 tcgttataca acatatacaa atgcgcggca ctccaggaga aatttggtgc gccttgctgt
    21181 gcgccagtca gcgggttgta attctcctga attgggccat cggcggttaa tcctttggcg
    21241 tgccggaaga acgtatccgc cagcttcagg gcatcatcgc gataaccgta acgctccatc
    21301 cctttcagac caaaccagaa ctgatccacc cacacgcgcc cgcgccagta aatatctgcg
    21361 ccaaaggccg gattggttaa cgccgccgtt cccagcggta caaaggtgtt gaactcttta
    21421 ggatcgagca tcaccttcac caccgcgtcg gcgttggcct gcgttgccgc accgttaaac
    21481 agcggcgacc agccttccgg ccctttgccg cgctcgacaa tcggtttgcc cgcgcagccg
    21541 ttcgccagtg gtttatcttc aatgcgcacg tcatagtaga actgggtagt cgggtcgaac
    21601 atacaggtgt tgatgtagtc cgcgagctgt tgtgctaact gacgatagcg tttggcctct
    21661 tccggcttac cgagaatcgt cgccatctcc gccagataat ggttatcgct gtacatatag
    21721 ctggcctgat ccaccgactc ctgcaatagc gagtagccca gcaacgttcc gtcctgactg
    21781 cggttttcgg cgaatttcac cgtccagtcg ctacgtttgc cgccgttagc gacatattta
    21841 tccagctgtt ctttgtcgat aaacccaaag acggcggcat catcacgccc cgattcccac
    21901 gacgccgcaa cctgtgccgg gatttccaga ctgtcgtact ggcctttctc taccacgcgg
    21961 gcgtagttgt tcagcccaga cagcgtctct gctttgttgc ctttcttcac cgtaaacagc
    22021 atctcgccgc tctcagtgtt gtgggctttg tcgcgggtcg cgccatattc cggcacgccg
    22081 ttgccgttgt gatcgcggtt acgtagccac cagtcgtgat aggccaccag tttcgggtac
    22141 atctctgcca gccaggtttt atcctgggtg acgttataca cttccattac tgaccaggcg
    22201 gcaaggctgg gtttggtatt acgtttgttc cagttgccgc catcgccgcc acgctcgggg
    22261 ctaaggttcc atgctatcag gtcggggaca aagcccacat cctgcggacg cacgcggtcg
    22321 cctggctgga tctgccagga gaagaccgcg cggatattct ctttggcgat atccggattg
    22381 aaatgcgcca tcgcaaatgc ctgcttccag gtatcccacg gccaggtctg attgccggag
    22441 aaccagcgcc cggtcaccga cggcgtgacg gtgttatatt tcaccgcccc cccaggcgag
    22501 cgccagttac cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg ttccggcgtc
    22561 gcatccggat tggttaaccc tttcttcaga tactcttccc agcgttgctg cgaggcggtg
    22621 agataaaacg ccggacgcgc cagaatatcg cggatctgca tttgctcttt gctaacttcc
    22681 tgagcggtca gcaggtggga gtaggtggta tagagcgtgg ccgaaccgtt gatatgcgcc
    22741 ttactggtaa agcgattacc gttgatttca gtctgcactg gcagggattt atgcacctga
    22801 tattctgatt caccggaggt cagcagatcc caggtggcgc gcactttgcc aaaggtgact
    22861 ttcaggccat cacgggtggc gctgattttg cgctgatagt cagggtattc gccagcaatg
    22921 gttttatcgg aaagcggttt cccttctttc gcttccagtt tttccagcag ctcgccgtcc
    22981 cacaccagat ccagcggttt attgctgatg attttagttt ccagtagtga cgtgcgcggc
    23041 gtggcgaagc gcagagtcat ttcgacctgc acatcttttg ctgtcagttt ttgcaccagc
    23101 gcgccgggaa tactgtatgc ctccagcgtg aagtcgactt tcttgccgtc ttgccagacg
    23161 gtcagacgat cgaaattgct ggccataaag ttgatgtatt cttccgtcag cagcgcaacg
    23221 cctggaaaac cgcccatggt gttcgggccg tctggcaaca gatgaccatg ccaggctccg
    23281 agatcgaaaa acggattaaa acgctggtga tcgtcgtaat cgtaatcctt catgtactgc
    23341 ggcgcgccag tacggttaat cacgttttta taattgtcgg cgttagcggc atgggcggaa
    23401 aaacttatca gcagagcgca ggttactggc gttaaaatag ttttgatttt catgaaatta
    23461 cagtccttat taccagtaga aatattgcat caggaagagt tggtcgctgg gatcgtgaga
    23521 ggcatttttc atgtagaagc ggctatcaaa agccgttgcg aaacgtccgc cgccgtattc
    23581 gtaccagata ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt cagggcgatg
    23641 tttggcgtag gtgtaagcgg tggagagata aatgcctttt aacgcttcat acatggcgct
    23701 caccatcatc ccgctttcac ttcccgatac gtccagcccg tcggcgcgtc cggtatggtg
    23761 ccaggcgcga gcggcaaagt taggggtaag caggcccgtc agatagagtt ggcctgtgcc
    23821 gtcggtgatt tccagaccgt tcatccaggt gatgccttcg gcaaggtcat actggatgta
    23881 accgttcatc attgccggat aggtgtattt gtcgtcgtag cgatcgtact tgccaaaatg
    23941 tagccaggct ttgctttcgt catgatgtcc ggcaggtgtg gcggtaacgc tataacgaaa
    24001 gttgccgctt aagttctgca ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac
    24061 gtagcccaga tcgggcgtga aatcgcccca ccattgcagg tcatccagcg aggaatcctg
    24121 acgcagactg agcatgattt cggtgccatc attggtacga taaccgccat agaaacggtt
    24181 aatcccacct tcgaaaccgc cccagctatg gtcaggcacc caggcattac cgtcgtggtc
    24241 ggcctgcacg gtccagcctt cgccgtaaat caggccgaac caggatccgt acttcatctc
    24301 caggccacct tcaatatagg tgccgtcgct gtatttatgt ttattctcac cctctaaatc
    24361 catataccca ccaaccccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt
    24421 cattgcgggg gtttttgtgt cgtcatgttc tgccggacgt gcttcatcag cggtcacaga
    24481 aaaggcgtag caaaacggca gcgcaagtaa tgctctgcat ggtgcagtga ttaatttcat
    24541 tgtgttaatc catgagctaa agaaaaagta cagcctttta tcgttgtaat tataaaaata
    24601 taaaggcaga aactattttc cctctcccca tccaggaaga gggaaaatgc ctgctcattt
    24661 tttattattc tgcttaatca acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag
    24721 ccgttaatcc ctttatatat ttactgtatt tccaccacgc aaagccgagg aagataacaa
    24781 taccgccgac gttataaaaa atgatggtca gaatattcgc gccagtcggg aatgtcgaag
    24841 cgacaaaccc tacggcaaat atcgcaatca gcattgaaac aacgataata ccggtgcggc
    24901 gggagcccat acggaaatcg cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag
    24961 ccagcatgat gaataacggc ggaagcatgg acgctgcggc ggtcatatta ataatggtat
    25021 tcatcagatc ttgcacggta ttggaaccga gcatcgggat aatcatcagc gggatgacga
    25081 tcaggaactg gatccacgct gcgcgcgccg gaacgccgtt ttcgttcagt tcgacggttt
    25141 tcttaccaaa gatgccttcc gggatttcgg agaagaaaat tttcaccggc gttgcggtcc
    25201 acatcagcag ggaaccgaac atcgcggtaa aggacaccag accgacaaag cgattcatca
    25261 acgcttccgg cagaccaaaa tacgccgcca tgccgtggaa tacctgcacc gatccgccag
    25321 taaatttcaa ctctttgctg ctgacgaaga cgttaatcag caccgaggaa acggaataca
    25381 gtacgccgat aaaaatcccg gcgaggatga tcactttaac gaacgatttc gaaccgcctt
    25441 tgacgtcgtt aacgtacacc gcgacggact ccgcgccacc tgcggccata aagatccagg
    25501 tggtaacgcc gaggaacgcc cagttgaagt tcgggatcat cgcgtcaacg gtgatcggat
    25561 cggcaggctg tacgccgcca accagtgccg tacctgccag taaaatgtag gagagcgtta
    25621 acagcagcat cagcgttgaa gtgacggagg taattggccc cagcattttc gccccgttgg
    25681 tggaaaccca ggtggagaag gcgaacagca ccatactgat aatggtggtg gcaaccggcg
    25741 tcataatata ttcgtagccg aggaaggcat acgaagcata agcaataacg cgcggcaaca
    25801 gtgaggtgaa aaagaacagg tttacgaacc agtaggtata ggcagtaata aatgcccaac
    25861 gcccgcccag cgaacttttt acccacgcgt agacaccggc ttctgagttt ttatttaacg
    25921 aaacaaattc tgcgatgatc agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa
    25981 acatcggtgc cgaggccagt ccaagctcaa tattattatt gataacgttg ttaaagctgt
    26041 aaacggcggc aaaagtcagc gagagcaagc cgaatttgcc gattgtatta cgtttggtat
    26101 cagacataac acatctccga aaatgagtag ggtagcggtg gcggcaaact ccttttgccg
    26161 ccgtagttgt tatttgttat ggaaataacc atcttcgatg gtgactttga gaaccacttt
    26221 tttgaccgcg ttattgcaga taaaccgata tgcttcatgg atatcgtaaa taacgatttg
    26281 cccttcgtgg acctctacgg tttctccgca gccttttaaa tattcacggt cagtttcatc
    26341 acgataatat tccacgacct gtaatgtctc tttcggcgca tattcaattt tttgctgccc
    26401 ttgcaggtag taatgcactt caaaatagcg tcgatgcccg gtaaatagcg catcggtcgc
    26461 ggagtccgtc tccacgcggt aagtcaatga gtcaccgatg gagtgggcga cgccaggctg
    26521 aatgttgtcg atattttcaa tcgcttcaac gcagcgttgc cacttcttgc cagaggcgta
    26581 aatctggcgg aactgttcta agttatcgat gatcctcatt gcttattctc gctgtgcaaa
    26641 ttcgtggaaa agaaccctgc gccgaactca tacgacgcca ggctttgcgc ggtagcttct
    26701 ccgccagaaa ccggcagcaa cgtaaagccg tagctgaagt cacggaacca gacgcgccag
    26761 gagtccagca cctcgctgcc ccaggagttg gagccgaggc caagcagctg gtggtcgaga
    26821 ttcagggtga tgtcatcact gcgctgcaac tcgttacagt gctgggcagc gtggatgttt
    26881 tcctgggtat agcgccaggc gctgaagtta atggggcgct gcggaacaac cagcagacca
    26941 ttgccgtggc ggttagtcag tgccgtccag cggacatgct gacggttgcc gttgttctgt
    27001 gggaagggat agttctcgaa catggcatcg acggtgctgc gccagatatc gatgatgtta
    27061 gcctgctggc tgtcggcgta gttttcgccc ggtccacgac cgtaatacgc cacctggtcg
    27121 tattcgccgt taatccccat ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg
    27181 taacgctcgc cggaaagcgc cacgttaacc tggccatcgg cagcgatgcg ccagatgtag
    27241 gtgcagcgca tcccgaagtc aaacaccggt ggggcaataa ccgtgcggct gatgatcaac
    27301 acttcaccat cgctctgttc tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa
    27361 tgattcggct gccacagccc ttcgtactcc tgcttgtggt tgtcgatcat tggcttgaag
    27421 aagttgattt ttggctcgcg ggttagcagc gactcgccat tcacctgcca ggatgtcggt
    27481 ttaccactcg tttttgagaa ggtgatcgca aagttgtagc cgcgaacagt acagctcaaa
    27541 cgatcgtctt ccagcgtcag cggacgggcg ttattcggtg cgaaaggtac aggctgcgcg
    27601 gtgttttcct tcagcgggaa ctgataagtg gcgatagaat gcccggcttc gctgtagcgg
    27661 gtgcgggaat ctttggtcac cgtaatgttg agaaacgctt cgcgggcgtc cagctgtggc
    27721 agcgtgatct gtaagggggc ttcgctgttc ggcgcaacgt cgcgcagttt aatctgttgc
    27781 gtcgcgagcg tttcaccttc ggcgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg
    27841 gtaaaccaca gattattttc gactttcaac tcgccgcgag tcagatccag cgcgtggatt
    27901 tttactggcg cgataacctg tttgtactct ttcaggcccg gtcccggcgt ctgatcggaa
    27961 tagatcaaac catcaagaca gaagttatag ttgttgggat agtcgccgta gtcgccgccg
    28021 aatttatacc agacattgcc gttgtcatcc tgcgcctgga tcccgtggtc gcaccattcc
    28081 cagacataat gtccctgaat gcaatcatgc ttatagaaga cgttctggta ctccgtcagc
    28141 ccgcccggtc cgttccccat cgcatgagca tattcacaga tgatgcgcgg cttcggatgc
    28201 gggtattcac caaactcatt catcagcggc acgcgggtgt acatggtgga aataatatcg
    28261 accacttcag catcgcgatc ttcttcgtaa tgcaccagtc gcgtgtcatc cagcgccttc
    28321 gccgcatggt acatcgcgcg gatgttacag ccatagccgg attcattgcc cagcgaccag
    28381 atgatgatcg acggatggtt tttctgcgcg tgaatatggc gaacaatgcg ctcgacgtaa
    28441 actttttccc actgcggatc gtcggtaata cggctgatat cgccgacatt agcaaagccg
    28501 tgcgattcga cgtcggtttc cgccatcaca aacaggccgt agatatcaca cagttcgtaa
    28561 aaacgtggat cgttcgggta gtgagcggta cgcacggagt tgatgttgtg ctgcttcatc
    28621 aactggagat ctttctcgac gcgatccatc ccaacggcgc ggcctttgcg atgatcgttg
    28681 tcgtggcggt tgacgccgtg cagcatcaca taacggttat tgatccagaa cagaccgtcg
    28741 cgcactttga tatcacggaa gccaactcgt tgtggcacca cttccagaac gttgccgtcg
    28801 gcgtctttca gcgtcatgac cagatggtaa agataagggg attctgctga ccattgctgc
    28861 ggctgttcga cagtaaaagc aaaactggcg ctggtcagtt tttcaattgc caaatgatca
    28921 atggtgctgc tgtgcaccac gtgttcgcca tcaaacaggg tatattccag cgtcgtgacg
    28981 acaggggagg cggcgagatt ttccagcacc acttcgcagg aaagtgtggc atcgcaatag
    29041 gcttcgtcaa agtcggtacg aacggtgaaa tcgttaatat gcgtgagttg ttttccgatc
    29101 agataaacat cgcggaaaat ccccgccgac caccacatat cctggtcttc cacgtaggta
    29161 gagtccgccc actgcatcac gcgcacacac aacaggttgt cgccggtttt aaccatcgcg
    29221 ctgatgtcaa actctgcggt caggcgactg cctttgctga accccacata ctgaccgtta
    29281 acgtagactt caaaataggt ttcgacaccg tcaaatttga tcagcgtctg tttaccctgc
    29341 cagccgtcgc tgagggtgaa aatgcgttga taggcaccgg ttgggttatc gctggggaca
    29401 aacggcacat cgatggggaa cggaaaacct tcgtcggtat attgcagttt gccgtgacct
    29461 tccatttgcc acatggcggg gacggtaata tgcccccagt cagccattaa ctcagaggtg
    29521 aaggcttctg gcacttgcag cggatggtca aaaaagtgga aattccactg accgcttaag
    29581 ggcagaaaca ggctgctggt ttcacgggca aaagtacgag cctgcgcaac agaatcatat
    29641 gaaaaaaagt acgcacgcgg cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag
    29701 cgattcatag ggcctcccgg tagagagaaa tcggaaccat ttatggtgat ggtagtttgt
    29761 gtaattgctg gggagtttag taaaaatttt actaaaaagt agcggataaa tgcatttact
    29821 ttaactggat cacgaaaata aacggcagca ctgtccggca aaaacgattg ccggacagcg
    29881 tgcaagggga tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagaccaac
    29941 agcggcagcg cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc
    30001 atcatctcgg aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg
    30061 atatcgttaa cgctgataag cgaaatatcc tgtgggatgt tcaggccacg ttcatgaatt
    30121 gcccgcagta cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct
    30181 tcccgcgcca gcatctgttt tgccagttca tagcccgacg aactggaaaa accgccgcgc
    30241 cagatatcct cttcgcgtac cacgtgtttc agacgaccat attccgcaaa ggcgacctca
    30301 cgaatatccg ccttgccagg caagtcttca ccgccaataa aaccaatacg attaacgccc
    30361 tgattgatat agaaatcgat gatttcttta ctgatacgtg ccagatcgat atccaccgca
    30421 tcgtaaccgc tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcacag
    30481 gcggcggcgc gcagggcgga cgtgggtttg ccgacaatta aaataccggt gacgttttta
    30541 atgtctggta agccgttgtg ttcataacag ttggtcagtt cgatcgccag cttttcgcac
    30601 tgagtttcaa tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgt
    30661 tggtagctgt agatagccag aatatggtgt tggttgactg cgcctgtctg gagtttacgg
    30721 gcgctactgg tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct
    30781 tctttcacat tcaatgtcgg atcgtcattc aagaccctgg atactgtcgc cagggatacg
    30841 ccagcttcga ttgcgatgtc ttttagtgtt gccatgttta ccttttttac tttccctgat
    30901 gtgcgccccc attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc
    30961 gtttctttga cgctgtaacg gtaacacagc ggcttgcgaa tgtgatcacg cctgcattct
    31021 ccatttttag cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttactcg
    31081 aagggaggaa acaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt
    31141 cggaaagatt gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt
    31201 tgatgtgggg gaaaaaacgc tgcaaaccgt gaccagcctg gtgccgtact acagcgaaga
    31261 agagctgatg ggaaaaacag tggtggtctt atgcaatttg caaaaagcga aaatgcgcgg
    31321 tgaaacgtcg gaatgcatgc tgttgtgtgc ggaaaccgat gacggcagtg aaagcgtgct
    31381 gttaacgccg gagcggatga tgcctgcggg cgtacgtatc gtataaaaag atcggatggc
    31441 gacgtcgtat cgccatccga tttgatacta cgcttcttcg acactgactc gcatggccgc
    31501 cagcgcctta cgcgccgctt tgatcacctg ttcacactgt tcaatggtca gtgtcagtgg
    31561 cggttcaatg cggatcgttt tagcgttgtt gagtgttccg gccaccagga cgcgctggcg
    31621 gaacatctcg ctggcaaagt tatagccgat ttcgttatca acaaactcaa tcgccatcaa
    31681 catcccttta ccacgcgctt cctgtaccag atcgggatat tcccgcgcca gttgacgaaa
    31741 accgtccagc aacatatcgc ctttttgttc agcctgagcc ggtaagttct gctccagcaa
    31801 cacattgatg gtcgccagcg ccgccgcaca ggccagcggg ttgccgccaa aggtggtggt
    31861 atgcaggaac gggttgtcga acagaacgga aaacacctct tcagtggcga tggttgcgcc
    31921 aatcggcatc acgccgccgc cgagggcttt tgccagacac aggatgtccg gctgtacgtt
    31981 ctcatgctcg caggcgaaca tcttgcccgt gcgccccata ccggtttgca cttcatcgag
    32041 gatcatcagt gcgccgaact catcgcacag cttacgcacg gcggtgagat atcccggcgg
    32101 cggcagaatg acgccacctt cgccctgaat cggttcgagg atcaccgcag caacatcatc
    32161 accggttttt ttgcactcgt taagagccgt gcgcatggct tcgatattgc caaacggtac
    32221 atgacgaaac cctggcagca gtggcataaa aggtttgcgg aaggtcgatt tcgccgtggc
    32281 cgacagcgcg ccaagtgatt taccgtggaa cgcgccgctg gtggcaataa aagtaaactt
    32341 gccgcgcggt gactgataag ccttcgccag cttcagcgcc gcttcaacgg attcggtgcc
    32401 gctattacag aagaagctgt atttcagttt accgggcgtt agcgccgcaa gggttttcgc
    32461 cagcatcgcc cgtaacggat cgagcagctc ctggctgtga agcggttgtt tagcaagttg
    32521 attctgtacg gcggaaacca caactggatt acggtgcccc acgttgaaaa ttccaaaacc
    32581 tcccaggcag tcgataaact cctgtccctg ggtgtcgaca agcgtattta aaccccccgc
    32641 ttgccactct acggctccgt aatccccgcc agcggtaaca gatttgcgat actctaaaaa
    32701 ccccggattg acatgctctt tgaagtattc aatcacctcc cggttaagtg ctttcatctc
    32761 ctcatgatcc agcgttcgct tctcaatgag attcagggcg tgggcgctgc acgctaaagc
    32821 cgatgcgctc gaaggtaacc tgttcaaaat atgctccgga ggctcgcgta tcacatgata
    32881 ccgatttaag tattgcaggg attgcgccac tccggctgcg atcgcgaaaa tcgggataaa
    32941 cacaaatgtg gttatcattt gcgcaatatt taatgctatt tgttaacatc tgttgttatt
    33001 ttgcacggtt tttgtcgggt atggatttgg cgattgctgc gcaaatgcac actttcaggg
    33061 caacaactgc ctgatttttg gg
//
LOCUS       DAZGAP010000030        32835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGAP010000030 DAZGAP010000000
VERSION     DAZGAP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.030"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..325)
                     /locus_tag="ACHLAM_001207"
     CDS             complement(<1..325)
                     /locus_tag="ACHLAM_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
                     /protein_id="HFO7852770.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKM"
     gene            complement(782..868)
                     /locus_tag="ACHLAM_001208"
     tRNA            complement(782..868)
                     /locus_tag="ACHLAM_001208"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Leu,seq:gag)
     gene            complement(883..1215)
                     /gene="secG"
                     /locus_tag="ACHLAM_001209"
     CDS             complement(883..1215)
                     /gene="secG"
                     /locus_tag="ACHLAM_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO7852771.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQIQPAAPA
                     KPTSDIPN"
     gene            complement(1443..2780)
                     /gene="glmM"
                     /locus_tag="ACHLAM_001210"
     CDS             complement(1443..2780)
                     /gene="glmM"
                     /locus_tag="ACHLAM_001210"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO7852772.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2773..3621)
                     /gene="folP"
                     /locus_tag="ACHLAM_001211"
     CDS             complement(2773..3621)
                     /gene="folP"
                     /locus_tag="ACHLAM_001211"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO7852773.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3711..5645)
                     /gene="ftsH"
                     /locus_tag="ACHLAM_001212"
     CDS             complement(3711..5645)
                     /gene="ftsH"
                     /locus_tag="ACHLAM_001212"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO7852774.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5745..6374)
                     /gene="rlmE"
                     /locus_tag="ACHLAM_001213"
     CDS             complement(5745..6374)
                     /gene="rlmE"
                     /locus_tag="ACHLAM_001213"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO7852775.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6500..6793
                     /gene="yhbY"
                     /locus_tag="ACHLAM_001214"
     CDS             6500..6793
                     /gene="yhbY"
                     /locus_tag="ACHLAM_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO7852776.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6949..7425)
                     /gene="greA"
                     /locus_tag="ACHLAM_001215"
     CDS             complement(6949..7425)
                     /gene="greA"
                     /locus_tag="ACHLAM_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO7852777.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7673..9106
                     /gene="dacB"
                     /locus_tag="ACHLAM_001216"
     CDS             7673..9106
                     /gene="dacB"
                     /locus_tag="ACHLAM_001216"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7852778.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9147..10319)
                     /gene="cgtA"
                     /locus_tag="ACHLAM_001217"
     CDS             complement(9147..10319)
                     /gene="cgtA"
                     /locus_tag="ACHLAM_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO7852779.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(10335..11300)
                     /gene="yhbE"
                     /locus_tag="ACHLAM_001218"
     CDS             complement(10335..11300)
                     /gene="yhbE"
                     /locus_tag="ACHLAM_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7852780.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLGPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11427..11684)
                     /gene="rpmA"
                     /locus_tag="ACHLAM_001219"
     CDS             complement(11427..11684)
                     /gene="rpmA"
                     /locus_tag="ACHLAM_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO7852781.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11705..12016)
                     /gene="rplU"
                     /locus_tag="ACHLAM_001220"
     CDS             complement(11705..12016)
                     /gene="rplU"
                     /locus_tag="ACHLAM_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO7852782.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12275..13246
                     /gene="ispB"
                     /locus_tag="ACHLAM_001221"
     CDS             12275..13246
                     /gene="ispB"
                     /locus_tag="ACHLAM_001221"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO7852783.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13474..13752
                     /gene="sfsB"
                     /locus_tag="ACHLAM_001222"
     CDS             13474..13752
                     /gene="sfsB"
                     /locus_tag="ACHLAM_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO7852784.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13800..15059)
                     /gene="murA"
                     /locus_tag="ACHLAM_001223"
     CDS             complement(13800..15059)
                     /gene="murA"
                     /locus_tag="ACHLAM_001223"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO7852785.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15114..15368)
                     /gene="ibaG"
                     /locus_tag="ACHLAM_001224"
     CDS             complement(15114..15368)
                     /gene="ibaG"
                     /locus_tag="ACHLAM_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO7852786.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15528..15821)
                     /gene="mlaB"
                     /locus_tag="ACHLAM_001225"
     CDS             complement(15528..15821)
                     /gene="mlaB"
                     /locus_tag="ACHLAM_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO7852787.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15821..16456)
                     /gene="mlaC"
                     /locus_tag="ACHLAM_001226"
     CDS             complement(15821..16456)
                     /gene="mlaC"
                     /locus_tag="ACHLAM_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO7852788.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16475..17026)
                     /gene="mlaD"
                     /locus_tag="ACHLAM_001227"
     CDS             complement(16475..17026)
                     /gene="mlaD"
                     /locus_tag="ACHLAM_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO7852789.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17031..17813)
                     /gene="mlaE"
                     /locus_tag="ACHLAM_001228"
     CDS             complement(17031..17813)
                     /gene="mlaE"
                     /locus_tag="ACHLAM_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO7852790.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17821..18630)
                     /gene="mlaF"
                     /locus_tag="ACHLAM_001229"
     CDS             complement(17821..18630)
                     /gene="mlaF"
                     /locus_tag="ACHLAM_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO7852791.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18840..19817
                     /gene="yrbG"
                     /locus_tag="ACHLAM_001230"
     CDS             18840..19817
                     /gene="yrbG"
                     /locus_tag="ACHLAM_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO7852792.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19831..20817
                     /gene="kdsD"
                     /locus_tag="ACHLAM_001231"
     CDS             19831..20817
                     /gene="kdsD"
                     /locus_tag="ACHLAM_001231"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO7852793.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGVTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20838..21404
                     /gene="kdsC"
                     /locus_tag="ACHLAM_001232"
     CDS             20838..21404
                     /gene="kdsC"
                     /locus_tag="ACHLAM_001232"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO7852794.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSIAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21401..21976
                     /gene="lptC"
                     /locus_tag="ACHLAM_001233"
     CDS             21401..21976
                     /gene="lptC"
                     /locus_tag="ACHLAM_001233"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO7852795.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21945..22502
                     /gene="lptA"
                     /locus_tag="ACHLAM_001234"
     CDS             21945..22502
                     /gene="lptA"
                     /locus_tag="ACHLAM_001234"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO7852796.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22509..23234
                     /gene="lptB"
                     /locus_tag="ACHLAM_001235"
     CDS             22509..23234
                     /gene="lptB"
                     /locus_tag="ACHLAM_001235"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO7852797.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23282..24715
                     /gene="rpoN"
                     /locus_tag="ACHLAM_001236"
     CDS             23282..24715
                     /gene="rpoN"
                     /locus_tag="ACHLAM_001236"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO7852798.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24738..25025
                     /gene="hpf"
                     /locus_tag="ACHLAM_001237"
     CDS             24738..25025
                     /gene="hpf"
                     /locus_tag="ACHLAM_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO7852799.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25143..25634
                     /gene="ptsN"
                     /locus_tag="ACHLAM_001238"
     CDS             25143..25634
                     /gene="ptsN"
                     /locus_tag="ACHLAM_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO7852800.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25680..26534
                     /gene="rapZ"
                     /locus_tag="ACHLAM_001239"
     CDS             25680..26534
                     /gene="rapZ"
                     /locus_tag="ACHLAM_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO7852801.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26531..26803
                     /gene="npr"
                     /locus_tag="ACHLAM_001240"
     CDS             26531..26803
                     /gene="npr"
                     /locus_tag="ACHLAM_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO7852802.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27016..27648
                     /gene="yrbL"
                     /locus_tag="ACHLAM_001241"
     CDS             27016..27648
                     /gene="yrbL"
                     /locus_tag="ACHLAM_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO7852803.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27645..28373)
                     /gene="mtgA"
                     /locus_tag="ACHLAM_001242"
     CDS             complement(27645..28373)
                     /gene="mtgA"
                     /locus_tag="ACHLAM_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO7852804.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(28370..29023)
                     /gene="elbB"
                     /locus_tag="ACHLAM_001243"
     CDS             complement(28370..29023)
                     /gene="elbB"
                     /locus_tag="ACHLAM_001243"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO7852805.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29253..31589)
                     /gene="arcB"
                     /locus_tag="ACHLAM_001244"
     CDS             complement(29253..31589)
                     /gene="arcB"
                     /locus_tag="ACHLAM_001244"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO7852806.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLALVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31685..32614)
                     /gene="yhcC"
                     /locus_tag="ACHLAM_001245"
     CDS             complement(31685..32614)
                     /gene="yhcC"
                     /locus_tag="ACHLAM_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO7852807.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQTECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
BASE COUNT         8203 a         8886 c         7897 g         7849 t
ORIGIN      
        1 gcattttgac aacttcatcc ggatcactct gcagcacact aatcgcaaat ccatcattga
       61 aagttgtacc aaaggtacac catgaataga ggctaatact catgtctgcc gctgtcagta
      121 acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt ttaacaacca
      181 ggatggtcag aacgaagaac agcaatcgct gaaccatatg cgctccggaa gaggcgacta
      241 agttcgtaat accaaaaaca ataacacaaa gatacaacaa ccaatgagat ttaaaacttc
      301 taacaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat ataaacaatt
      361 atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat atcagaccga
      421 aacactgtta gttttttctt tttaaatgat gcggccattg ttctcagtaa ttaaataaca
      481 ttatcaatgc gtctctaaac attacgcaga acgtagccat cagtagttat actgacagga
      541 gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt accggaactg gagttgacac
      601 aataaagtgc caattatgtc agcagaaggg aaaagtttat aactaaggcg taccgatacc
      661 atcgtttcaa agtaccagct acgagtaaag caactggacg agatacagat acctgacaac
      721 cattcctcag accaggacca aaacgaaaaa agacgctttt cagcgtctct tttctggaat
      781 ttggtaccga ggacgggact tgaacccgta agccctattg ggcactacca cctcaaggta
      841 gcgtgtctac caattccacc acctcggcac ggatactact ttttagttcg ggatatcgct
      901 ggtcggctta gccggagctg ctggctgaat ttgttcggtt ttcgccggcg cactcaggtt
      961 ttcccattcg ctacctttat tggttttgtt gctattgatg ttacccagca ccagactgat
     1021 gatgaagaat aacgttgcca gcagcgctgt catgcgggtc atgaagttac cagaaccact
     1081 tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag gctcccatat cagcgccttt
     1141 accttgctgc agcatgatca gaccaacaag gccaattgcc acaataagga aaactactaa
     1201 aagagcttcg tacataatca acctgttcct tgcggagttg ccgcgtacca atgcttcaac
     1261 caataaagcg ggagttttta atctttccca ctgaagcggg tgtgaatact aaccaaagcg
     1321 aatgaccttc gcaagggcaa ttttattgca ttgtatcaac tgcggaaaaa aacagcaaaa
     1381 ccgatgtgtt cgctgagaaa aaaggcgaca atcgccgcct ttttagccag ttatttatcg
     1441 ttttaaacgg cttttactgc atcggcgatg cggtgtgcaa attcagtcac ctgcgcttcg
     1501 tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc cggatttacg caacagcaca
     1561 cgtccacggc tacccagcgc agcttcaact tctgcggtca cagctttgac tgactcatgc
     1621 tccagcggat cgccgctgcc tgctgtgtaa cgcacgttaa ccagaatctg cgggaacatt
     1681 ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac gcgccatcgc cgccagcacc
     1741 tgcaagccag caacaatgcc gtcaccggta gtggttttat ccagcaggat cacatgaccg
     1801 gagttctctg caccgatacg ccagcctttc tcctgcattt tttccagtac gtagcggtca
     1861 cccactttcg cgcgcgcaaa tggaatacct aactgtttca gcgccagttc aagccccatg
     1921 ttgctcatca atgtacccac agcaccacca cgcagctggc cctgacgaag accttcacgc
     1981 gcgatgatat acatgatctg atcgccatcg actttattgc cttcatggtc aaccataatc
     2041 acgcgatcgc cgtcgccgtc gaaggcaata ccgagatccg ctttttcagc cagcacacga
     2101 gcctggagcg cgcgaacgtc ggtagccccc acttcggcat tgatgtttac accgttcggc
     2161 tcacaaccga tagcgataac gttcgccccc agttcgcgca gcacgttcgg cgcgatgtga
     2221 taagtcgcac cgtttgcaca atccaccaca atcttcagtt cactgaggct aagttcgttc
     2281 gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcag catcaacgat acggctggct
     2341 ttacccagtt ctgccgaatc aacgcagctg atctcttttt ccatttccgc ttcgatggcc
     2401 tcttctaccg catccggtag tttggtgccg tcgatagaga agaatttaat gccgttatcg
     2461 tagaatgggt tatgcgatgc agaaattaca attccagcct ctgcgcggaa ggtacgcgtc
     2521 agataagcca cggccggtgt tggcatcggg ccagtgaaga gcgcggaaag accggctgcc
     2581 gcaagccccg cttccagtgc tgactccagc atatagccag aaatacgcgt gtctttacca
     2641 ataataatct tacgggagcc gtggcgcgcc agcactttac ccgcggccca acccagctta
     2701 agcacaaaat caggggtgat cggcgcatcc cctacacgac cacgaatccc atcggtaccg
     2761 aaatatttac gattactcat agcgtttgtt ttcctttgca gacagagtgg cttccaccac
     2821 ccgcatcgct tctacggttt ctttgacgtc atgaacacga atgatgtgcg cgccttgcat
     2881 tgcggcaatg accgcacagg ccagactacc gctcaaacgc tcggacggcc ccacgttcag
     2941 cagctgtcca atcatcgatt ttcgtgacat acccaccaac agcggcaggt tgaaatggtg
     3001 aaattcagcc aggcgcgcca gtaatgaata gttatgggag agatttttac cgaaaccgaa
     3061 tccggggtcg agcaacaatt tctcttttgc gatacccgcc tgctcgcaac gtgctatttg
     3121 ctcaataaag tagcgattca cttctgcaaa gacatcgtga tacttcgggg cttcctgcat
     3181 ggtttttgga tttccctgca tatgcatcag acaaaccggt aaaccggttt ctgcagccgc
     3241 ctccagagcg ccaggttcgg aaagggagcg gatatcgtta ataatgtgag cgccaacttt
     3301 cgctgactca cggatgactt ctggtttgga tgtatctacc gagatccaga cttcgaagcg
     3361 ttgagcaatt gcctcaacca caggaataac acgttgtaac tcttcttcaa cgctaacttc
     3421 cgccgcccct ggacgtgtgg actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat
     3481 catcagattc gcatgtttca ccgcatctat cagcgagtta tgcgtgccac catccgaaaa
     3541 ggaatcaggc gtgacgttga ggatccccat tacgtgagga tggctaaggt ccagtgaagt
     3601 accctgggca aagagtttca taatgttatc cctggtatga attgataaga aaaaaccccg
     3661 gagcacgccc cggggttttt ggtacaaata cagtcatctg atgcgggaac ttacttgtcg
     3721 cctaactgct ctgacatggt gttacccggg ttcggcgtac gcggttcatc aaccggacga
     3781 ggagcctttg gactaccatt gtcgccagaa ttgttagaag cgcctggttc ttcccagccc
     3841 gctggcggac gtacatcgcg acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc
     3901 tcatatttca tgagagcatc tttcatcgca tgcagtatat ccatattgtc ggtcagaagc
     3961 tgacgcgcac gattatagtt gcgctcaatc agtgctttca cttcctggtc gatgatacgt
     4021 gcagtttcat cggacatatg tttcgccttc gctacgctac ggccgaggaa cacttcacct
     4081 tcttcttccg cgtacagcag tggacccaat ttctcagaga agccccactg agtcaccatg
     4141 ttacgtgcca ggttggtcgc aactttaata tcgttggacg caccggtaga aacatgttcc
     4201 ggcccgtaga tgatctcttc tgccagacga ccaccgtaca gcgtagaaat ctggctttcc
     4261 agtttctgac ggctagcgct gattgcgtcg ccctcaggca agaagaaagt cacacccagc
     4321 gcacgaccgc gtgggataat cgtcactttg tgcaccggat catgttccgg caccaggcga
     4381 ccgataatcg catgacccgc ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc
     4441 accatggagc gacgttccgc acccatcatg attttgtctt tcgctttctc gaactcaacc
     4501 atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg ccgcttcgtt caccaggttc
     4561 gccaggtcag caccggagaa accaggagta ccacgggcaa tgattgccgc gtcgatatcg
     4621 ggtgccaatg gtacgcgacg catgtgaact ttcaggatct gttcacgacc gcgaacatct
     4681 ggcaagccaa ccacaacctg acggtcgaaa cggccaggac gcagcagcgc cgggtcgaga
     4741 acgtccggac ggttagtcgc ggcgataacg atgatacctt cgttaccttc gaagccatcc
     4801 atctcaacca gcatctggtt cagagtctgt tcacgttcat cgtgaccacc gcccagacct
     4861 gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa agatgatgca cggtgccgct
     4921 ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac ccacaccgac gaacatttct
     4981 acgaagtcag aaccggagat agtaaagaac ggaactttcg cttcgcctgc aatcgctttc
     5041 gccagcaacg ttttaccggt acccggagga ccgaccatca ggacgccttt cgggatctta
     5101 ccgccgagtt tctggaagcg gctcggctcg cgcagatact caaccagttc agcaacttct
     5161 tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg ttttgatctg atcttcagtc
     5221 agcatgcgcg ctttgctctt accaaacgac atggcacctt tgccaccgcc gccctgcatt
     5281 tgacgcatga agaagatcca gacaccaatc agcaacagca tcgggaacca ggagatgaag
     5341 atagaagcca gcaggcttgg ttcttcaggc ggttcaccga caaccttgac gttcttggtc
     5401 aacaagttat ccagtaattt cggatcctga accggaatgt aagtggtata acggttacta
     5461 tctttcttgg taacgttgat ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta
     5521 ttgacctctt gtaggaaggt agagtaatcc accttacggc cattagactc gctgggccca
     5581 aagctctgga atactgacat cagcacaacg gcaatgacca gccagagtat taggtttttc
     5641 gccatgtcac tcaagggatt aacctcttat tacaactgtg ttaaaaacag cgtcaggata
     5701 ctctatatcc agcatctttc aaactttcgt ctgaaatctc ccggttaggg tttacgcccg
     5761 gtcgctacaa tatacacttc ccgcgaacgt gcgcgagaag agtccggctt acgaactttg
     5821 accttcgtaa acagggagcg aatttccctt agatactcat cgaaaccttc gccctggaac
     5881 accttcacta caaaactgcc acctggcgct aatacatcac gacacatttc tagcgccagt
     5941 tccaccagat acatggcacg ggggatatcc accgccggtg ttccgctcat gtttggtgcc
     6001 atatcggaca tgacaacctg gactttgctg tcgccaacgc gctccagcag tgctttcatc
     6061 accagttcat cacgaaaatc gccctgaaga aagtccacac caacgatagg atccataggt
     6121 aaaagatcgc aagcgatgat acggccttta ccgccaattt gggtgaccac atattgtgac
     6181 caaccacccg gcgcagcacc aaggtcgaca accgtcattc ccggtttaaa gagtttgtca
     6241 ctttgctgta tttcatcaag tttaaaccag gcacgggaac gtaacccctt tttctgtgcc
     6301 tgttgaacat atttatcgct aaagtgttcc tgaagccagc ggctggagct ggcagaacgc
     6361 ttcttacctg tcatttaact ttcccattgg gataactcat cgtaaccaat tgcgtaaatt
     6421 tttacgcgcc tatttggcga tatatgggag atggcggtag aatgacccgt tttcaatccc
     6481 aacgtaagca aaaaatacga tgaatctgag tactaaacaa aaacagcacc tgaaaggtct
     6541 ggcacatccg ctcaagccag ttgttctgct tggcagtaat ggtttgaccg aaggggtgct
     6601 ggccgagatt gaacaagcgt tagagcacca tgaactcatc aaggtgaaaa tcgccaccga
     6661 agatcgcgaa actaaaacct tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa
     6721 tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca actaaagaac gtaaaatctc
     6781 gctgccacgc taagattatc ctaaagttac acacatttgc tgtgtaaaac gaggggtttt
     6841 ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga taaaaggccg catagcggcc
     6901 tttttccttt ctttacaata catcaacatc ttgagtattg ggtaattctt acaggtattc
     6961 caccttaatt acttcaaatt ctacttcgcc gcccggcgtt ttgatgacca caacatcatc
     7021 ttcttctttg ccgatcaggc cacgagcaat aggcgagttt acagaaatca ggttttgttt
     7081 aaagtcagct tcgtcatcgc caacgatgcg ataagtctgt tcttcgtcag aatccagatt
     7141 cagcaccgtt acggtagcac caaaaataac gcgcccattg ttgggcattt tggtgacatc
     7201 aatcacctgc gcgttcgaca gcttggcttc gatgtctttg atacggcctt cgcagaagcc
     7261 ctgctgttca cgagctgcgt ggtattcggc gttttctttc aggtcgccat gctcacgcgc
     7321 ttccgcgata gcagcaatga tttcaggacg gcgcacagat ttcagaaaat ccagctcttc
     7381 gcgtaatttt tcagcgccgc gtaaggtcat cggaatagct tgcatttgtt atacctcttg
     7441 aatattcctg atggggcaag tcttcaccca ccccggctgt tcggcctgcc cggcataact
     7501 cctcaccggg accagaagca aaaaaatacc gacccgggta caagtcccag gtcagctaca
     7561 attcacattt tgatagtcat tttaccctga agttcccgaa gggtcatcgt ttactttata
     7621 gggcgttgcg ccgtagtatg acggctcgat tccaggttgt tagcgcgaga ttatgcgatt
     7681 ttccagattt atcatcggat tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt
     7741 tgatgagtac attactcaac tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt
     7801 cggcgcgtcg gcccccgcta ttgattacca cagtcagcag atggcgctgc ccgccagtac
     7861 gcagaaagtg attactgcac tggcggcgtt gattcaactc ggccccgatt ttcgttttac
     7921 cacgacgctt gaaaccaaag gcaatgtgga aaacggcgta cttaagggtg acttagtggc
     7981 gcgatttggt gccgatccga cgttaagacg tcaggatatt cgcaatatgg tcgcgacttt
     8041 gaaaaaatct ggcgtcaacc aaatcgatgg caatgtgttg atagatacct ccattttcgc
     8101 cagccacgat aaagcccccg gctggccatg gaatgacatg acacaatgct ttagcgctcc
     8161 gcctgccgcc gccatagttg accgcaactg tttctccgtc tcgctctata gtgccccaaa
     8221 acctggtgat atggctttta tacgcgtggc atcttattac cccgttacga tgttcagcca
     8281 ggtacgcacc ctcccccgtg gttctgccga agcgcaatac tgcgagctgg atgtggtgcc
     8341 aggcgacctg aaccgcttta cgctgaccgg atgcctgcca caacgttctg agccgctccc
     8401 gttggctttt gccgtgcagg atggtgccag ctatgctgga gcaattctga aagatgagtt
     8461 aaaacaggca ggtatcacct ggagcggaac actgctgcgt cagacgcagg ttaacgaacc
     8521 cggaacggta gttgccagta aacagtcggc cccgttgcac gatctgctta agattatgct
     8581 gaaaaagtcg gacaacatga ttgccgatac ggttttccgc atgatcggcc atgcgcgctt
     8641 caatgtgcct ggaacatggc gggccgggtc ggacgccgtg cgtcagatcc tgcgccagca
     8701 agccggtgtc gatattggaa ataccattat tgccgatggt tcagggcttt cgcggcataa
     8761 cctgattgct cccgccacca tgatgcaggt gctgcaatac attgcccaac acgacaatga
     8821 acttaacttt atctctatgc tgccgctggc gggctatgac ggctctttgc agtaccgtgc
     8881 aggtctgcat caggcgggcg tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg
     8941 ggtatataac ctggcgggat tcattaccac agcgagcggg caacgaatgg cgtttgtgca
     9001 atatctttct ggctatgcag tagaaccggc ggatcagcgt aatcgccgta ttccgttggt
     9061 acgttttgaa agccgtttgt ataaggatat ttaccagaac aattagtcta aagagaaacc
     9121 ccggcacatg gccggggttt tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg
     9181 tcgtcttcgt cccagtcgtc atcccagtct tcatcatcct cttcagcaat ctcttcaagc
     9241 tgctggcgat gataatcatc ccacatgaat tcgactttct ctggctgttt cgcttcttca
     9301 gcctgcacga ccgggttttc aatgataaag gtcatcacat cccagcagag atctttcacg
     9361 cccagtccgc tcgccgcaga gatcagataa tatttatctt cccaacccag cgcctcagcg
     9421 atcgctttcg ctttctcttc ggcttctgcc ttatccagca ggtcgatctt gttgaagact
     9481 aaccaacgcg gtttcgccgc cagatcctgg ctgtattttt ccagctcgct gataataata
     9541 cgcgcgtttt caaccggatc ggtgccatca atcggatcga tatcgatgag gtgcaacagg
     9601 acgcggcaac gctccaggtg cttcaggaag cgaatgccca gacctgcgcc ttctgcagcg
     9661 ccttcaatca gtcctggaat atcggcaaca acgaagctct tttcgttgtc cattcgtacc
     9721 acaccaagac ttggcaccag agtggtaaac ggataatccg ccactttcgg tttagccgcc
     9781 gatactgcgc ggataaatgt cgatttaccc gcgtttggca tccccagcat accgacgtca
     9841 gccagcagca tcagctccag tagcagctca cgcttatcgc ccggcgtacc gttagttttc
     9901 tgccgtggtg tacggttaac tgacgatttg aaacgggtat tgcccagacc gtgccagccg
     9961 cccttagcaa ccagcagacg ctgaccgtgt ttggtcatat cgcccatggt ttcaccggta
    10021 ccctggtcga taacacgcgt acctaccggc actttaatcg tcacgtcttt accgcgctta
    10081 ccggtacagt cgcggcttgc gccattctga ccacgctctg cgcggaaaga tttttcaaaa
    10141 cgataatcga taagcgtgtt caggttctcg tcggcttcca tccatacgtc accgccgtca
    10201 ccgccgtcgc cgccatccgg gccgcctttc ggaatatact tttcgcggcg gaagctcacg
    10261 caaccattac cgccatcacc tgcaacgacc agaatcgatg cttcatcaac aaacttcatt
    10321 ttattctccg taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt
    10381 aatccgcccc aaatacgatg accaatggcg gaatacatcg cgcccgcaac cacgacaaac
    10441 gcaccgagat aacctaaaag gtttaacatc ggtctggcga agaaatcggg ccaggccagt
    10501 gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga gcgtaatgat cgcgctcacc
    10561 tgcgctgcct gccagcgagc catcgcttcc gccagggcgc catatcctac caatgtattc
    10621 agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa gctgcgctat cactccaggc
    10681 tttgccagag ggaagagcgc aattgtacat aaagtgtaca gcaaaaacag gatctgcggt
    10741 gaggccagtc gacgcaataa aaccttttgc gccacgccat aactcaccca caccgtcgcc
    10801 gcaccgaccc caaagattac tccccaggtg taatcggtga gctttgtaaa tatctcgacc
    10861 agactggtgt taaaaaacat caccaggccg ctcaggagca tcaatgcccc tacaacctga
    10921 gtgctgcgca ttttctcttt caggataaat acgctggcaa ccatcatgcc aactggcgag
    10981 agttgcccaa tcacctgcga agcggtcgga cccaggtatt gcaaggatga gctgaacagg
    11041 atgaagttcc caaacagccc ggcggtcgcc actgccaaca aaatcaacca gcgtggctta
    11101 cgaaacacgc gtaatggcgg caaccgcttc ttcaccgcaa gaatggcacc caggccaata
    11161 ctcgccatca agaaacggta aaacacgatt gtcggaggtt ccatcacctc cagcacctgc
    11221 ttcattgcga ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa aagaatgcca
    11281 atgcctgcct gctgcttcat accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc
    11341 accatgcgca tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt
    11401 tcatccgtta ccgggacgcg aaaaacttat tcagcttcga tgctgataaa tttacggttt
    11461 ttcgggcctt taacttcgaa tttcacttta ccgtctgctt tagcaaacag agtgtggtca
    11521 cgaccgcaac ctacgttagc gccagcgtgg aatttggtac cacgttgacg aacgatgatg
    11581 ctacccgcca gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa
    11641 tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa tctctcctca
    11701 ggtcttaggc gctgatgcca gtaattttca catcagtgaa ccactgacga tggccctgct
    11761 gcttacgata gtgtttacga cgacgaaact taacgatttt aactttctcg ccacgaccgt
    11821 gagcaacaac ttcagctttg attacgccgc catcaacgaa aggaacgccg attttgactt
    11881 cttcaccgtt tgcgatcatc agcacttcag cgaactcaac agtttcgcca gttgcgatgt
    11941 ccagcttttc caggcgaacg gtctgacctt cgcttactcg gtgttgttta ccaccacttt
    12001 ggaaaaccgc gtacatataa aactccgctt ccgcgcacac cttttcaatg attcagagtg
    12061 cgctataaat attcacaata gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg
    12121 ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg gtaacgttta tctgtgccat
    12181 tttttcagta caatcaccct atattcctaa ccataaaccc taagttgcct ttgttcacag
    12241 taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt
    12301 aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt gagcagctta attccgacgt
    12361 ccaactgatc aatcagttag gctattacat cgtcagcggc ggcggtaaac gtattcgtcc
    12421 gatgattgct gtactggctg cacgagctgt tggctatgag ggaaatgcgc atgtcaccat
    12481 tgctgccctg atcgagttta tccacacggc gactctgctg cacgacgacg ttgtggatga
    12541 atcagatatg cgcaggggta aagctaccgc caacgctgca tttggcaatg ccgccagcgt
    12601 gctggtaggc gattttattt atacccgcgc gttccagatg atgaccagcc tcggctcgct
    12661 caaagtgctg gaagtcatgt cagaagccgt aaacgtcatc gcagaaggtg aagttctgca
    12721 actgatgaac gttaacgatc cggacatcac tgaagaaaac tacatgcgcg ttatctatag
    12781 caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc gggattctgg ctggctgtac
    12841 gccggaggag gagaaaggcc tgcaggatta tgggcgctat ctcggcactg ctttccagtt
    12901 gatcgacgat ttactcgatt acaatgccga tggcgaacag ttaggtaaaa acgtcggcga
    12961 cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat gcaatgcatc atggcacacc
    13021 agaacaggca cagatgattc gtaccgccat cgaacagggt aacggtcgcc atcttctgga
    13081 accggttctg gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc agcgtgccga
    13141 ggaagaagca gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc cgtggcgaga
    13201 agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc ctcccctcat
    13261 cccgcgcagc gcgcgggatg attccagtaa attccataaa aacacttatt cagctctata
    13321 acacctgaaa aatagggacg tacatcttta tatattctga atattcacac tctttacagg
    13381 aactttttag agcaataggc catcaggagt atagtgatgc tcgacagaag aagtgttctg
    13441 aatgaaagcg actaacttaa ggagtgagga aagatggaaa gtaatttcat tgactggcat
    13501 cccgctgaca tcattgcggg tttgcgcaag aagggaacgt caatggcggc ggaatctcgc
    13561 agaaatggtt tgagttcctc aacgctggcg aacgcattat cgcgcccctg gccgaaagga
    13621 gagatgatta ttgcgaaagc cctgggaact gacccctgga ttatctggcc atctcgctac
    13681 catgatccgc agacccatga gtttatcgac agaacgcagt tgatgcgtag ctacactaaa
    13741 ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg gctgccagct ctcagacaat
    13801 tactcgcctt tcacacgctc aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt
    13861 tcgtagccac gatcgatgtg ataaatacga tcaactaccg tcgtcccttc cgcaatacag
    13921 ccagccagca ccaggcttgc tgatgcacgc agatcggttg ccataacctg tgcgccagaa
    13981 agtttttcaa caccgtgaca aataacggta ttgctttcga tttcggcgtg cgcgcccata
    14041 cggctcagct ctggcacatg cataaagcgg ttttcaaaga ccgtttcggt gatgaagccg
    14101 gtcccttctg ccaccaggtt caacagcgtg aactgggcct gcatatcggt cgggaatgcc
    14161 ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt tgccatgcat atccaggcta
    14221 atccagtctt cgccgacttc gatgtctgct ccagcgtcac gcagtttcgc cagcacggcg
    14281 tcgagagtat ctggctgcgc gttacggcag ataattttgc cgcgagagat cgccgccgcc
    14341 accaggaaag taccggtttc gatacgatcc ggcagcacgc gatagacacc gccgcctaaa
    14401 cgttccacac cttcgatgac gatacgatcg gtgccctgac cgctaatttt cgcacccagc
    14461 gtaatcagga agttcgcggt atcgacgatt tccggttcac gcgctgcgtt ttcaataatc
    14521 gtggtgcctt ccgccagggt tgcagcacac atgatggtca ccgttgcgcc aacgctgact
    14581 ttatccatca cgatatgtgc gcctttcaga cgaccatcga cggaagcttt aacgtaacct
    14641 tcttccagtt tgatggtcgc gcctaattgt tcgagaccag aaatgtgtag atcaaccgga
    14701 cgtgcaccaa tcgtacaacc gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc
    14761 agcggcccca gcgcccagat agaagcacgc atggttttaa ccagatcgta aggtgcgcag
    14821 aatacattaa cgtcgcgggc atcaatatgc acagaaccat tacgttctac tttcgcaccc
    14881 agctggctta gcagcttcat tgatgtatcg acgtctttta gtttcgggac gttctggatc
    14941 tctaccggtt cttccgccag tagtgcggca aaaaggatag gcagagcagc atttttagcg
    15001 ccggaaattg tgacttcgcc ctggagcttc gttggcccct gaacacgaaa tttatccatt
    15061 tagtttgttc tcagttaaca attcatatcc gctaccggcg aatcgcccat agctcaaaag
    15121 ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat acgctttgat cgacacagca
    15181 tgaatgcggt tatccgcaat atattccatc agcgggccat agaccgtctg ctgtttttta
    15241 acccgactca tgccgtcaaa caactcaccc acggcaataa cctgaaagtg gctgccatcg
    15301 ccggaaacgt ggacttcctg gagggagaga gcgttcatca acacgctctg aatttcatta
    15361 ttttccatgg gatcttcaat catcagttaa taaaccagcg aaacatctta gagcaaagtt
    15421 gcgctggcat aaataagcaa aaagcctcgc tgataaatca gacaaggctc gacttgcagg
    15481 caggtttgcc ggacaggcgg ttaacgccat atccggcctg aaaaaattta acgaggcaga
    15541 acatcagcag gcaaattata caatttcgcc agggtataca ctttgtcgtt taccccctga
    15601 agcgtcacat tgttgccctg ctttttcgcc agatcgataa gatggagcag cagcgccagt
    15661 ccacctgtat caacgcggga tacacggcta agatcgatac aggtaatccc cttcaccgct
    15721 tcctcacgca tttcccaaag cggtagcaga acgtcctgat ccagctctcc ggataacgcc
    15781 agcgtgtcac ccgtctgcat ccagctcagt gactcgctca ttattttttc tcttccagag
    15841 tgattttctg ttgagaaatc gatttcagtt gcgcagtcag gccgtcaata cctttggtac
    15901 gcagcagcgt tccccactcg ttttgtttgg tggtgatcat actgacgcct tcagcaatca
    15961 tgtcgtaagc ctgccagttg cccgtctggg agtttttacg ccactggaag tccagacgca
    16021 ccggcgggcg gccattcggg tcaataatgg taacgcgaat aggcacaatg gttttatcac
    16081 ccagcggctg ttctggcgca atctgatagg tttgaccgtg atacatcgcc agcgcctgac
    16141 cgtaagcctg cttcaggtac tcacggaaag cggcaaagta agcatcacgt tgagctgggg
    16201 tcgcactctt gtaatactga cccagcacca gcgcaccggc gtatttcacc tgtacgtatg
    16261 gcagcagttc ctgatcaaca atggtacgca gataatccgg gttggcccga atttgtggtt
    16321 gctcattctt caggcgatcg aacgttttct gcgccgcctc gtccatcagc ttatacggat
    16381 tggtctggtc tgccgcggtt gccgcactca gcggtgcaat caccagcaaa gcgaccatca
    16441 ttaaacgttt aaacatgcgt cggttctcct gaaattattt cgttgtaccc acaggttcag
    16501 tggtttcatt attacctggc gcagcagctg gcgcatcgcc actattctta ttgtcatcgc
    16561 ctttactacc gtaaaggaac tgaccaataa gatcttccag caccatcgca gacttggtgt
    16621 cctgaattgt atcgccatcc ttcaggatag cagtccccag ttccgggtct tcaaaaccga
    16681 cgtttaatgc cagatattgt tcccccagca ggccggaagt acgaatgctc agcgaactgg
    16741 tatcaggaat gtggttataa cgttgttcaa tttccagcgt tacgcgcggc agataggttt
    16801 tcgggtccag cgtaatatcc gccacccggc ccacaacaac accaccaatg ctgaccggag
    16861 agcgcgcttt caggccgcca atgttatcga acgtcgcata aagcgtgtag gtcggttcag
    16921 tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa cagcgccgcc agtagtgctg
    16981 ctaataaaaa gatacccacc caaatttcat tttttttcgt ttgcatgaac tcaattccca
    17041 aacatcaatg cggtcagcac aaaatccagc cccagaacag ccagagacga gtggacaacg
    17101 gtgcgagtgg ttgcccggct aatcccggca gacgtcggga tggcgtcgta gccgttaaac
    17161 aacgaaatcc acgtcaccgt gatggcaaac accacgctct taatcagaca gttgaccaga
    17221 tccatacgcc agtcgacggc attttgcatt gccgaccaga agaacccgct atcaatgcct
    17281 ttccaactga cgccgaccag agatccgccc cagatcccca cggcgacgaa aataaccgtc
    17341 aacagtggta atgaaataac cccagcccag aaacggggag aaataacccg acgcagcgga
    17401 tccaccgcca tcatctccat actggagagt tgctctgtag cgcgcatcag gccgatttct
    17461 gcggttagcg ccgaaccagc acgcccggca aacaacaacg cggcaaccac cggccccagt
    17521 tcacgcagta gcgataacgc caccagcata cccagactgg tttccgcact ataagtggtc
    17581 agaaccagat aaccttgcag ccccaacacc attccgatga acacgccaga aaccacaata
    17641 atcagcatcg acaggacgcc gacattatag agctggcgca ccagcagcgg cgcatgtttg
    17701 cgaaattccg gtttgccgac cagcgcattg aataacatta acccggcccg cccgaacgtt
    17761 ctcagggttt taatcccttt atgtccgagc gacgccagcg catttaacag catgagtggc
    17821 ttaactccct ggtaaaagat cagcgtgata atcgccggca ggatagcgga acggaacagg
    17881 cccgtcagct atcccgtcca gaaactgacg tacgcgtgga tcaggattcg cctgcaacgc
    17941 ctgggcactg ccatgagcga caattttttt gtccgccagg atccaggcgt gatcggcaat
    18001 acttaacact tccggcacat cgtgagaaac caccacacaa gtcacaccca gcgcgctgtt
    18061 cagctcagaa atcagcttca ccagtacgcc catggtgatg ggatcttgcc caacaaaggg
    18121 ttcatcaaac atgatgagat ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg
    18181 cgccatccca ccggaaagtt cagaaggcat tagtttagcc gctccacgca gccccacggc
    18241 ctccagcttc atcatcaccg tactatgcaa caatggcgcg ggaagttggg tatgttcgcg
    18301 cagtggatag gcgacgttgt caaatacgtt catatcagtg aacaacgccc ctgactgaaa
    18361 taacatgctc atccgtttgc gcactgtata caggcgcgaa cgagacatcg ccggaatatt
    18421 ctcaccatcg aaaagaatct caccatgatc tggtgcgatt tgcccgccaa tcagacggag
    18481 tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc gtgatcttcc ctcgcggcac
    18541 ggtcagggaa atattatcga agatgcagcg attgccacgc gtaaaactga catcgcgcat
    18601 atcgactaaa ttcgccacag actgctccat aattcaccct tcgtcttgcg ttgattttct
    18661 aagcatggcg ctcaatttaa ccttgaaccc aacatattta cagaatatta cccgccgtgg
    18721 ttagcgaaag ctggcatttg ttttactttt tagccgcata aagtcaaaat taagaatccg
    18781 ttacggcttt ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa ggacgttaga
    18841 tgcttttagc tacggcactg ttaattgttg gtttactttt ggtcgtttac agtgccgacc
    18901 gcctggtttt tgccgcgtct attctttgcc gaacctttgg catcccgccg ctaatcatcg
    18961 gcatgacggt ggtcagtatt ggtacatcgt taccagaaat catcgtctcg cttgccgcgt
    19021 ctctgcacga acaacgcgat ttagccgttg gtacagccct cggctcaaac attatcaata
    19081 tattgctgat cctcggtctg gctgcgctgg ttcgtccttt taccgtccat tctgatgttc
    19141 tacgccgtga attaccctta atgttgttgg tcagcgtggt ggccggttcc gtactctatg
    19201 acggacaact tagtcgaagc gatggtatct ttctcctctt tctggctgtg ctatggctgc
    19261 tgttcattgt taaacttgca cgtcaggctg aacgtcaggg gactgacagc ctgaccagag
    19321 agcagcttgc agagctgcct cgtgacggcg gattgcccgt cgcgttttta tggctcggca
    19381 ttgcgcttat catcatgcca gtggccacgc ggatggtggt tgataacgcc acggtgctgg
    19441 cgaattactt tgccatcagc gagttgacga tgggtctgac ggcaattgct atcggaacca
    19501 gcctgccgga actggcaacc gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg
    19561 tcggaaatat cattggcgca aacattttta atattgtcat cgtattgggt ttacccgcgc
    19621 tgataacgcc aggagagatt gatccactgg cgtacagtcg tgactacagc gtgatgttgc
    19681 tggtgagcat tatttttgcg ttgctgtgct ggcggcgctc cccgcaaccg ggccgtggtg
    19741 taggggtatt attaactggc ggatttatcg tatggctggc gatgttgtac tggttatcgc
    19801 caatactcgt tgaataactg gaaacgcatt atgtcgcacg tagagttaca accgggtttt
    19861 gactttcagc aagcaggtaa agaagtcctg gcgattgaac gtgaatgcct ggcggagctt
    19921 gatcaataca tcaatcagaa tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg
    19981 aaagttgtcg tcatggggat ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg
    20041 tttgccagca ccggtacacc ttcatttttc gtccatcctg gtgaagccgc gcatggtgat
    20101 ttaggcatgg tcaccccaca ggatgtggtg attgctatct ctaactcagg tgaatccagc
    20161 gaaatcacgg ccttaattcc agtgcttaag cgtcttcacg taccgttaat ctgcatcacc
    20221 ggtcgcccgg agagcagcat ggcgcgcgcc gcagatgtgc atctgtgtgt taaagtagcg
    20281 aaagaagcct gtccgttagg gctggcaccg accagcagca ccaccgccac gctggttatg
    20341 ggcgatgccc tcgctgtcgc gctgttaaaa gcacgcggct ttactgctga agattttgcg
    20401 ctctcacacc caggcggcgc actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg
    20461 catacgggcg atgagatccc gcatgttaag aaaacggcca gtctgcgtga cgcattgctg
    20521 gaagttaccc gcaaaaatct tggtgtgact gtcatttgcg atgacaatat gatgattgaa
    20581 ggcatcttta ccgacggtga tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag
    20641 ttaagtattg ccgatgtgat gacgccgggg ggaatacgtg tgcgccctgg cattctggcc
    20701 gttgaggcac tgaacttaat gcagtcccgc catatcacct ccgtgatggt tgccgatggc
    20761 gaccatttac tcggtgtgtt acatatgcat gatttactgc gtgcaggcgt agtgtaaaga
    20821 ttcaaggata aacaacaatg agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg
    20881 tcagcgccga cgttatagca aaagcagaga acattcgtct gctgatcctc gatgtcgatg
    20941 gcgtactgtc agatggcctg atttatatgg gcaataatgg cgaagagctg aaagcgttca
    21001 atgttcgtga cggttatggc attcgttgtg cgctcacctc tgatattgaa gtcgctatca
    21061 ttaccgggcg aaaggctaaa ctggtagaag atcgttgtgc cacattgggg atcactcact
    21121 tgtatcaggg gcagtcaaac aaactgatcg cctttagcga tttgctggaa aaactggcga
    21181 ttgccccgga aaatgtggct tatgtcggcg atgatctcat cgactggccg gtaatggaaa
    21241 aagtgggttt aagcatcgcc gtggccgatg cgcatccact gttaatcccg cgcgccgatt
    21301 acgtgacgcg cattgctggc ggtcgtggtg cagtgcgtga agtttgcgac ttattactcc
    21361 tggcgcaggg caaactggat gaagccaaag ggcaatcgat atgagtaaag ccagacgttg
    21421 ggttatcatt gtgctgtcac tggcggttct ggtgatgatc ggcattaata tggccgaaaa
    21481 agacgatacc gcccaggtgg tcgtcaacaa caatgatccc acctataaaa gcgagcatac
    21541 ggacacgctc gtctataacc cagaaggggc actaagctat cgattgattg ctcaacacgt
    21601 tgaatattat tccgatcagg ccgtttcgtg gtttacgcag ccggtactta ccacgtttga
    21661 taaggataaa atcccgacat ggtccgtaaa agcagataaa gccaagctga ccaatgaccg
    21721 gatgctctat ttatatggac acgttgaagt caacgcactc gtgccggact ctcaacttcg
    21781 cagaatcacg acggataacg cgcagatcaa tctggtgacg caggatgtta cctctgaaga
    21841 cctcgtcacg ttatacggaa caacatttaa ctccagcggt ctgaaaatgc gcggcaactt
    21901 acgcagcaag aacgccgagc tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa
    21961 acaaactcag ccttaatctt gtgcttgcca gctcacttct ggccgccagc attccggcat
    22021 ttgccgtaac cggagacact gatcagccga tccacattga atcggaccag caatctcttg
    22081 atatgcaagg caacgtggtt acctttaccg gcaatgtcat cgtcacccag ggcaccatca
    22141 aaattaatgc cgacaaagtg gtcgttaccc gtccgggcgg cgaacaaggt aaagaagtga
    22201 ttgacggcta cggtaaaccg gcaacgttct accagatgca ggacaacggt aaacccgttg
    22261 aaggtcacgc ttcacagatg cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta
    22321 atgcttatct gcagcaggtc gatagcaaca ttaagggcga taagatcact tacctggtga
    22381 aagagcagaa aatgcaggct ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc
    22441 cgtcgcagct gcaggacaaa aacaataaag gccagacccc ggcacagaag aagggtaatt
    22501 aattcgttat ggcaacatta actgcaaaga accttgcaaa agcctataaa ggccgtcgcg
    22561 tggtagaaga cgtcagcctg accgtcaact ccggggaaat tgtcggtctg ctggggccaa
    22621 acggtgccgg taagaccacc actttctaca tggttgttgg cattgtgccg cgcgatgcgg
    22681 gcaacatcat tattgatgat gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc
    22741 gcggtatcgg ctatctgccg caggaagcct ccattttccg tcgcctcagc gtttacgata
    22801 acctgatggc ggtactgcaa attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg
    22861 cgaacgagct gatggaagag tttcacattg agcacctgcg tgacagcatg gggcagtcac
    22921 tctccggggg tgaacgtcgc cgtgtagaaa ttgcccgtgc actggctgcg aatccgaaat
    22981 ttattctgct cgacgaaccg tttgccgggg ttgacccgat ctcggttatc gacattaaac
    23041 gcatcattga gcacctgcgc gacagcggcc tgggcgtact gatcactgac cacaacgtgc
    23101 gtgaaacact ggcggtttgt gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc
    23161 acggcacgcc tacagaaatc ttacaagacg aacacgttaa gcgtgtatac cttggggaag
    23221 acttcagact ctgatagggt agaagtttgc gatgttttag caggagagta cgattctgaa
    23281 catgaagcaa ggtttgcaac tcaggcttag ccaacaactg gcgatgacgc cacagctcca
    23341 acaggcaatt cgtctgttgc agttgtcgac gctggaactt cagcaggagc tacagcaggc
    23401 gctggagagt aatccgctgc ttgagcaaat cgacactcat gaagaaatcg acacccgcga
    23461 aacgcaagac agtgaaacac tggacaccgc cgacgcgctc gaacaaaaag agatgccgga
    23521 agagctgccg ctcgatgcca gttgggacac catttacacc gctggtacac catccggcac
    23581 cagcggtgac tacattgacg acgagctgcc ggtctatcag ggcgaaacga cgcagacctt
    23641 gcaggattac ctgatgtggc aggtcgagct gacaccgttt tccgacactg accgcgctat
    23701 tgctacctct atcgtcgatg ccgttgatga caccggttat ctgactgtcc cgctggaaga
    23761 tattctcgaa agtatgggcg atgaagagat tgacatcgac gaagttgaag ccgtccttaa
    23821 gcggatccaa cggtttgatc cggtcggtgt ggcggcaaaa gatctgcgtg actgtctgct
    23881 gatccaactc tcccaattcg ataaaaccac gccatggctg gaagaggcca gactgatcat
    23941 tagcgatcat ctcgatctgt tagccaatca cgacttccgc actttaatgc gcgtcacgcg
    24001 gctgaaagaa gatgtgctga aagaagccgt caatctgatc cagtcgctcg atccgcgccc
    24061 cgggcagtcg atccagacgg gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa
    24121 gcataacggt cactggacgg tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa
    24181 ccagcactac gcctcgatgt gcaataacgc gcgcaacgat ggtgacagcc agtttatccg
    24241 cagcaatctg caggatgcca aatggttgat caagagcctg gaaagccgta acgatacgct
    24301 actgcgcgtg agtcgctgta tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga
    24361 atatatgaaa ccgatggtac tggccgatat cgcccaggct gtcgaaatgc atgaatcgac
    24421 gatatctcgc gtgaccacgc aaaaatacct gcatagtcca cgaggcattt ttgaactgaa
    24481 gtatttcttt tccagtcacg tcaataccga gggcggcggc gaagcttcct ccacggcgat
    24541 tcgtgcgctg gtgaagaaat taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag
    24601 caagttaacc tctttgctgt cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa
    24661 gtaccgagag tctttatcca ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa
    24721 ccgataagga agacactatg cagctcaaca ttaccggaaa taacgtcgag atcaccgagg
    24781 cactgcgcga atttgttaca gccaaatttg ccaaacttga gcaatatttt gaccgaatca
    24841 accaggtcta tgttgttctg aaagtggaga aagtcaccca cacctcagat gcaacactgc
    24901 atgtaaacgg tggtgaaatt catgccagcg cggaaggtca ggacatgtac gctgccattg
    24961 atggcttaat tgataagctg gcacgccagt tgaccaaaca taaagataaa ctgaaacaac
    25021 actaattgtc cgggcaatta gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat
    25081 ttgtacggtt aatgctccga gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa
    25141 ttatgacaaa taatgataca actctacagc ttagcagtgt tcttaacagg gaatgtacgc
    25201 gaagccgcgt ccactgtcag agcaaaaaac gcgccctgga aatcatcagc gagctggcgg
    25261 cgaaacaact tagtctgccg cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa
    25321 tgggcagtac cggtatcggc aatggtattg ccattccgca tggcaaactg gaagaagata
    25381 ctctgcgcgc cgttggcgtt ttcgttcagc tcgaaacgcc tatagctttc gatgccattg
    25441 acaaccaacc ggtagattta ctatttgcgt tgctggttcc ggctgaccaa accaaaacgc
    25501 acctgcatac tctgtcgctg gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt
    25561 tacgcgcagc ccagagcgat gaagagctgt atcaaatcat tacggatacc gaaggtactc
    25621 cggatgaagc gtagttattc ggtaatgtct cttttagacg ttgtgaggag aaacagtaca
    25681 tggtactgat gatcgtcagc ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc
    25741 tggaagatat gggtttttac tgcgtggata accttcccgt agtgttgtta cccgatctgg
    25801 ctcgaaccct ggccgatcgc gagatttctg ccgccgtcag cattgatgtt cgtaatatgc
    25861 cggagtcacc agaaatattc gaacaggcga tgagtaacct gcctgacgct ttctcaccgc
    25921 aactcctgtt cctggatgcc gaccgtaata ccttaattcg tcgttacagt gacacgcgcc
    25981 gactgcatcc gctttccagc aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg
    26041 atttgctgga gcctctgcgt tcgcgagcgg atctgattgt cgatacctca gaaatgtccg
    26101 ttcacgagct ggcagaaatg ctgcgtaccc gtctgctggg taaacgtgaa cgtgaactga
    26161 ccatggtctt tgagtctttc ggcttcaaac acggtatccc tatcgatgca gattacgtct
    26221 ttgacgtgcg cttcttgccg aacccgcact gggatccgaa actgcgtcca atgacaggtc
    26281 ttgataaacc tgtcgccgcg ttcctcgacc gccacacaga agtacacaat tttatctacc
    26341 agacgcgaag ctatcttgag ctatggttac ctatgctgga aaccaacaac cgtagctacc
    26401 tgacggtcgc cattggttgt accggcggga agcaccgttc ggtgtatatt gcagagcaac
    26461 tggcagacta cttccgctcg cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa
    26521 aacgtaaacc atgaccgtca agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc
    26581 ccggcctgca atgaagctgt ttgaattaat gcaaggtttt gacgcagaag tgctgttacg
    26641 taatgacgaa ggtaccgagg ccgaagccaa cagcgttatt gcactgctga tgctggattc
    26701 tgccaaagga cggcagattg aagttgaagc gaccggtcca caggaagagg aagcactggc
    26761 cgccgttatc gccctcttta attctggttt tgatgaagat taatcttcat cactttgaca
    26821 ttcaaatccc ttcaagctcc cccgacaata agaaaagcgc gtgctgaaat cgttctcaat
    26881 caacgtcatt tgtacatttt gtgcgctttt cactttcaga agaaccttaa gaaaacctta
    26941 agaagcattg tttaggtttc gtttaagtta atcgaccata ctggagatcg tcagaaaata
    27001 tttccaggag atggcatgat tcgcttatct gaacaaagtc ccctgggcac cggaagacat
    27061 cggaaatgtt atgcgcatcc ggaagatgcc caacgctgta tcaagattgt ctaccatcgt
    27121 ggcgatggtg gcgataaaga gatccgccgt gagttaaagt actacgcgca tcttggtcgc
    27181 cggttaaaag actggagtgg aataccgcgc tatcacggta ccgttgagac ggattgcgga
    27241 accggttatg tctacgatgt gattgcagat tttgacggca aaccctccat tacgctgacc
    27301 gaatttgctg aacaatgccg ttacgaagaa gacattgcac aactgcgcca gctactgaaa
    27361 cagctgaagc gttatttgca ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat
    27421 atcctctgtc accgcatcag tgagtcagag gttatccccg tcgtctgcga caacattggt
    27481 gaaagcacat tgattccact ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag
    27541 cggttatgga aacgatttat cgcccaaccg gcgctggcta ttgccctgca gaaagatctg
    27601 caacctcgtg agagcaaaac gctggccctc acttcgcgcg aagcttaatc cagctggtgc
    27661 tgttgcataa acgactcacc gcctaactga tacatctgcc gtaaaatcca cgcctgacgg
    27721 ctacgcacgt agcccgatgg cgcggagact ttgaaacgaa gtggattagg taatacagca
    27781 gccagtaatg cagcttccga gcgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt
    27841 gccgcagctt cgacgccaaa cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc
    27901 aggatacgct ttttgctcca gactgtttct atccccagcg ttaatccagc ttccagccct
    27961 tttcgtaccc agctacgccc atcccataaa aagagatttt tggccgtctg ctgagaaatc
    28021 gttgaagcac cgcgaatacg gttttcattg cgctcgttgt gcgccagggc tttctcaatg
    28081 gaagcgacat caaagcccca gtgctcagga aatttctgat cttctgcggc aatcaccgcc
    28141 agtcccatcc acggcgagat ttgatccata ctgacccagt cagaatgtgc cacgtaacga
    28201 aaattgccat gcagccaggc gctgacctgt cgctcgacca ttaccgctga gaagggaaca
    28261 ggcgcaacac taaacaacgc gatgcccccg ccccagaaaa cggcgaggac aaccattaac
    28321 cgcaaaagaa aacggcgaac gaaactaaac accgttaagc ggcttttact cattcagcca
    28381 gaaccagcac gcgggaaacc agcttatcaa tgccgctcgc cgcttctgca atgttctgcg
    28441 ccagcatata tgctggggtg gtgacaatct tattgtcttc atcaaccacg atatcatcga
    28501 caggacatgg cacatgctcc gcgcccatct cttccagcac ttctgcggta tcgatatcag
    28561 taccgatggt caaacgcagc gggaaatcga aaattttcgg cagcatcgcc ggggcaatac
    28621 acataaaacc aagcggtttt ccggcctgat gcatcgcttg tgccagcgcc tttaattcac
    28681 ggtcaacggt gcattcgcta ccaagactgg caaaattgct taaattcttc gccgcgccaa
    28741 aaccccccgg cacaatcaac gcatccagtt cagcggcatc ggcctgggcc agaggacgga
    28801 tttcaccgcg cgttattcgt gccgcttcaa tcagcacatt gcgcgtttcc gtcatcgctt
    28861 cgcccgttaa atggttgata acatcaacct gctgcttatc cggtgcaaag cagaccgcct
    28921 gcgcaccgct gcgtgaaata gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt
    28981 catagacgcc gcatccgctc agaattacgc caattttctt cattgtgatc atcctttcgc
    29041 aattgactga aacacattaa tttttttaat aaaaatggta cgcatcacac atttaactga
    29101 ttcatgtaac aaatcattta agttttgcta tcttaactgc gtgcggcctg aaaaacagtg
    29161 ctgtgccctt gtaactcatc ataataattt acggcgcagc caagatttcc ctggtgttgg
    29221 cgcagtattc gcgcaccccg gtctagccgg ggtcattttt tagtggcttt tgccacccac
    29281 gctttcagca cttctacgtc gtgacgccac tcttctttca tctcttcaat ccattcaccg
    29341 acgttatctt cccaggcagg aaggtcagga gactgaattt gctgacccag ctgttgcaga
    29401 tggcgtaacc ccactgaccc cgccgcacct ttaattttat gtccttcctc aacaatgcct
    29461 tttttatcct gcgccgtcag attcgactcc agcacgctga catagcccgg catcatcttc
    29521 tcaaacaccg ctaacccgtc ggtgatcagc ttcggtccta caagtgcgag atactgttcc
    29581 agcatgggaa tatcgagcaa tgcttctgat ttactgttct cttctgtcgt caccgtactc
    29641 tcctcatcat cctgggtatc ccagaatttc ttgatcatcg cggttagcgc cggaaccgaa
    29701 agcggcttac tcagcacatc atccattcca gcattgaggt actcttgttt gtctttcagc
    29761 acgttagcgg ttaaggccac cagcggcggt aaatcctcgc gcggataacg cttcgtcagt
    29821 tcacgagaga tatccagccc ggtcatatct ggcaactgaa tatccagcaa caccaggtcg
    29881 tattcgcccg gtttaaacat ctccagcgcc gccttgccgg tcatggcgac atcaacgctg
    29941 ttacctaatt tttccagcac agaacgcgca acaatcacgt tcagttcaat gtcttccacc
    30001 agcagcacat tcagcgccgg taaaggcata tcgtcttcat caaacgcatc atcgacctct
    30061 tctgctaccg acggtgcgtg gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg
    30121 gtaaccgtaa tatcgccgcc catatttttc gccagacgac gagaaacggc cagaccaata
    30181 ccggtgccgg tggcaggttt accgccatga ctgtctttca cctggtaata catggcgaaa
    30241 attttatcca gctcatcctg cggaatgccg ataccagagt cttccacttc aaaatgcagc
    30301 atatcgcctt catcgtagcg cacgcgcacg gtaacctggc cttgctgggt gaatttgacg
    30361 gcgttactga tgagattcca caggatctgc cgtaaacgcg tcccgtcggt aatgacctga
    30421 tgcggtaatg gcagcgtagg ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat
    30481 gcggaaagat tttccagatc ggcaaggaag ctggtgaaat caaccggctg attatcaagc
    30541 tggaccttac gccgttccat cttatccatg tcgataatat cgttgaagat attcccgagc
    30601 gtgacggccg aaacatggat ggttttgaga tatttttcct gctcggcggt gagttcggta
    30661 tccagcagaa tgcggctcag gccgacgata ccattcagcg gtgtacgcaa ttcgtgactg
    30721 atggtggaga taaacgtcgt tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc
    30781 ttacgctcgg taatgtcgcg accaaagccc atcaaaccgt gacgtttacc cacgcggtcg
    30841 tagtacggca ctttacggat ttcaaagcag gctttgcgtc cgtcgggata atccagccac
    30901 tgctcatagg tcagtgacac attatgacgg aacacttttt catcggtttc aatgactttc
    30961 gcagcggctt ccggtgagta aacatcagca ggtttcagat gaaccagttg tttttcgctt
    31021 tttccggtca gcagctccat cgcgcggtta cagccggaaa actctttatc ttcgttacga
    31081 taaaaaacca ggtcgggtga agcgtcaagg aaggaacgta agaatgagga ttgctgctcg
    31141 agctgaattt gtgtctcttc gcgctctttg atttcaattt tcagttggcc gaaggtttcc
    31201 tgcagttctg cttccgcttt ttcacgaacg gcgatttcct gatttagctg ggcaatatta
    31261 tcttttaact gaacgttgag gctcaaatcg cgctcgcgca tctcctccag tttttgcacc
    31321 agtcgtgaca gacgttgtcg tgactcctcc agttgctcga cgaccaccga tagaaagtag
    31381 accgcccacg gcgtaatcag caaaccaaag aagatagaac gaataacatc aatgctttcg
    31441 acctgaccat gcagcaccat ggttaccgcc atttgtacca caatggcaag aacgacgagg
    31501 gccagcgcca gtaacattga gaagcgcacc agacctaact tcatcatcag gtcaacataa
    31561 tactgcgcca gcagacgaat ttgcttcatt agggaattcc ttcacgacaa cctgccccaa
    31621 taatacccaa ttctgacagt tacgttataa attgtgtgag aaatgcggaa ttcccctcaa
    31681 tggcctacgc cgttggaggt agccacggac gtcccagcgc cgatccctgt acgccatgtt
    31741 cattcagata acgatccagc tcgaccattc ccgtccatcg attttcgcac cacagcggag
    31801 caagcagcgt cgggcgacgg gcgctggcgg aaatgcggtg gtagatcact tccggtggcg
    31861 tatggcgaat catttctccg gcagtgagcg tgtaatcctc cagttcaatt ccgttcaaac
    31921 gccccgcttc ccaggctttc gccataatac tgcctttcac aatatgcagc ggatgcagct
    31981 ttatgccgtc cacgccggtt tcaaccactc gctccagcgt ttgcaaacat tccgtctgcc
    32041 cttcgccagg caggccgaca attaagtggg aacacacctt cagcccgcgc tgacgtgcca
    32101 gctgggttgt acgctgataa caggcaaaat catggccgcg gttgatgcga tgcagcgttt
    32161 tgtcgtgggc ggtttgtagc cccagctcca gccacacttc gtaaccctgg tccttatatt
    32221 cgcaaagcag atccagcacc gcatccggca cgcagtccgg gcgggtacca acacacaaac
    32281 cgacaatatt ggcctggctc accgcctgct gatacataga acgcagcacc tgaacttccg
    32341 caaaggtgct ggtatacgcc tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat
    32401 tcgcctggtg cgccagttgc tcggcaatgg aacgatgctg ctgcgcttca tcggcaaacg
    32461 aggccacatt acagaatgtg cagccaccac gcccgatggt accgtcacgg ttagggcagc
    32521 taaaaccgcc gtgcagcgtc agcttatgca ccttttgccc ataacgacgg gtgagatcac
    32581 caccaaacat attgactaat ttctgtaact gcataatctg atagaccgcg ccttgaaaag
    32641 aggccaaagc ctgccatttt tagcccaatt cggcgatgac ctggatcaat cgtcccggcc
    32701 cgcttttatc aactgcataa tcaatcaaaa ttaccgaaat ttcatgcaca atcacataaa
    32761 tcacttttgc ttatcttgtg tcagattttt ttatctcctt atggatttta ggcaaaaaca
    32821 gtagcatgaa acgtt
//
LOCUS       DAZGAP010000031        32328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGAP010000031 DAZGAP010000000
VERSION     DAZGAP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.031"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(121..1518)
                     /gene="ccp"
                     /locus_tag="ACHLAM_001246"
     CDS             complement(121..1518)
                     /gene="ccp"
                     /locus_tag="ACHLAM_001246"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO7852808.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1923..3572
                     /locus_tag="ACHLAM_001247"
     CDS             1923..3572
                     /locus_tag="ACHLAM_001247"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7852809.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3623..4225)
                     /locus_tag="ACHLAM_001248"
     CDS             complement(3623..4225)
                     /locus_tag="ACHLAM_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO7852810.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4745..5644
                     /locus_tag="ACHLAM_001249"
     CDS             4745..5644
                     /locus_tag="ACHLAM_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7852811.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5693..6706
                     /gene="yhjD"
                     /locus_tag="ACHLAM_001250"
     CDS             5693..6706
                     /gene="yhjD"
                     /locus_tag="ACHLAM_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO7852812.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7099..8421
                     /locus_tag="ACHLAM_001251"
     CDS             7099..8421
                     /locus_tag="ACHLAM_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7852813.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8600..10659)
                     /locus_tag="ACHLAM_001252"
                     /pseudo
     CDS             complement(8600..10659)
                     /locus_tag="ACHLAM_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
     gene            complement(10729..11496)
                     /gene="pdeH"
                     /locus_tag="ACHLAM_001253"
     CDS             complement(10729..11496)
                     /gene="pdeH"
                     /locus_tag="ACHLAM_001253"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO7852814.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11728..12657
                     /gene="kdgK"
                     /locus_tag="ACHLAM_001254"
     CDS             11728..12657
                     /gene="kdgK"
                     /locus_tag="ACHLAM_001254"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO7852815.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12753..14249)
                     /locus_tag="ACHLAM_001255"
     CDS             complement(12753..14249)
                     /locus_tag="ACHLAM_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7852816.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(14470..15756)
                     /gene="dctA"
                     /locus_tag="ACHLAM_001256"
     CDS             complement(14470..15756)
                     /gene="dctA"
                     /locus_tag="ACHLAM_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO7852817.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15939..17927)
                     /gene="hmsP"
                     /locus_tag="ACHLAM_001257"
     CDS             complement(15939..17927)
                     /gene="hmsP"
                     /locus_tag="ACHLAM_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO7852818.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18009..21500)
                     /gene="bcsC"
                     /locus_tag="ACHLAM_001258"
     CDS             complement(18009..21500)
                     /gene="bcsC"
                     /locus_tag="ACHLAM_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO7852819.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQAGQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQW
                     SSNIQELVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRD
                     YTAARAAYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRR
                     VALAQAQLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETY
                     KDAMVASGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDY
                     WGSSGTGGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGT
                     CTLQDCSGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLG
                     YTVNAHRRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLS
                     GDQLTGKNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGY
                     YSPQEYLSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAA
                     RQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGW
                     QGDMDLPPQPLIPYADW"
     gene            complement(21482..22588)
                     /gene="bcsZ"
                     /locus_tag="ACHLAM_001259"
     CDS             complement(21482..22588)
                     /gene="bcsZ"
                     /locus_tag="ACHLAM_001259"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO7852820.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22595..24934)
                     /gene="bcsB"
                     /locus_tag="ACHLAM_001260"
     CDS             complement(22595..24934)
                     /gene="bcsB"
                     /locus_tag="ACHLAM_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO7852821.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24945..27563)
                     /gene="bcsA"
                     /locus_tag="ACHLAM_001261"
     CDS             complement(24945..27563)
                     /gene="bcsA"
                     /locus_tag="ACHLAM_001261"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO7852822.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27560..28312)
                     /gene="bcsQ"
                     /locus_tag="ACHLAM_001262"
     CDS             complement(27560..28312)
                     /gene="bcsQ"
                     /locus_tag="ACHLAM_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO7852823.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICTGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28324..28512)
                     /gene="bcsR"
                     /locus_tag="ACHLAM_001263"
     CDS             complement(28324..28512)
                     /gene="bcsR"
                     /locus_tag="ACHLAM_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO7852824.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28795..30366
                     /gene="bcsE"
                     /locus_tag="ACHLAM_001264"
     CDS             28795..30366
                     /gene="bcsE"
                     /locus_tag="ACHLAM_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO7852825.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30363..30554
                     /gene="bcsF"
                     /locus_tag="ACHLAM_001265"
     CDS             30363..30554
                     /gene="bcsF"
                     /locus_tag="ACHLAM_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO7852826.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30551..32230
                     /gene="bcsG"
                     /locus_tag="ACHLAM_001266"
     CDS             30551..32230
                     /gene="bcsG"
                     /locus_tag="ACHLAM_001266"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO7852827.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
BASE COUNT         7630 a         8835 c         8106 g         7757 t
ORIGIN      
        1 aaatcctaag cagaaaaacg accttttata atataaacat ttaatatgat aatattaaaa
       61 acagacttta ttatcgtaat aaaaatcgcc caaacactcc tgttcgggcg atcaaattaa
      121 ttattgttta tcctgcatat acggcgtgta aaccccgttc agactgtgca ggaaagctac
      181 gatatcatcc acatcctcct gcggcagctc tttgcctacc tgatagcgca gcatcagttt
      241 caccgccccg tccagcgtcg gcacgtcacc gcgatggaag tacggtgccg ttaaagcaac
      301 gttacgtaaa ccgggtactt tctgacgcaa tttatcacgc tcttctttag taacattcat
      361 acgaccaata tccgccgccg taatttcacc aaagttaaag tcttttttca gccccaacgg
      421 ttcaaaggaa cgtccgccga gaataatacc accatgacaa gttgcacatt tattatcttt
      481 aaataattga tagccttttt tctgttgcgc cgtcagagca ttctcatctc cacgcaacca
      541 tttatcaaat ggggaatccg gcgtaattaa tgttttctca aattcagcaa tggcatcagt
      601 aatattttcg ccactgaaac cttgtggata gacttcgagg aactgcgctt taagctgcgg
      661 atctttttcc agcttagcaa taatttcgtc ccaggatttc gacgccattt caatcgggtt
      721 caacggcggt ccaccagcct gatcctgcaa tgttgccgca cgaccatccc agaactgctc
      781 aacgttaaat actgagttaa ataccgtcgg cgcgttaatc ggcccaactg cgccaccaac
      841 accaatcgat gtttttctgc catcgacgcc ccccgcattc aacgcatggc aatgagcgca
      901 tgaaatggtg ctatcagccg ataaacgggg atcgtgatac agcgcaaaac ccaacgccac
      961 tttttgcgca tcggtaggca gtttttgcgg gatgggctgc accggttcat tgcgatgctc
     1021 cggagcagta tcattgctgg cgtaatattc cgcgcgctgt tttgcaatcc aggccagtat
     1081 ttccgcccgc tcttcatcac tcaccttacc cgcccagtgt agcgcggtat aacgcgttgg
     1141 cggcatagtt tcatactgca tcacccattc aatcttattc agatcgcttt gcgaaacggg
     1201 tttattagcc agcagagccg cacgcacggc ctcgaggtta aaagatttat atccaagctt
     1261 tatgtcgtaa tccatcaact gtttcgcgcc gggaatataa taataggcgg gtaattctgc
     1321 cgaaggtgta tggcaatagt cgcatccttt ttcacggaga aagcctaaaa ccttattatt
     1381 ttcactgaca gcagatgcct gaacatcggc ctgtttactg cgtttattat cgtggtacca
     1441 cacataacca gataacccta aatagcaaat cgcgacgcca gccaggccga tcgcggtaat
     1501 acgtgagacc attttcatta ttcttccctc acggtggtga gttattgtga cccagaaaat
     1561 tttccagaat gctgcaaacg tctctgcgag acaaagccat cataaaaaac agggagatat
     1621 gctttttgat aggaatcaat caattacatg aatataaact atcaataaga tagccagtgc
     1681 ctattaccca gatcaggaat ggcatatcat gattgcggcg agagcaggat tgaatgttct
     1741 gaaaatgaaa aacaacacga aatccgtatt tttataaatt ccattgatat tagtgcgtta
     1801 tattttttgg caggttaata aacgtgatcc accgcacgct ttgtcgccca ccaggcggag
     1861 cgaatgacta cccttaaaga aaagcccgat aattagcgac gaatttcgga ggtcggatcc
     1921 ttatgctcaa tcagaaaatt caaaacccta atccagacga actgatgatc gaggtcgatc
     1981 tctgctatga actggaccca tatgaattaa aactggatga gatgatcgag gcagaaccgg
     2041 aacccgagat gattgaaggg ctgcccgcct ctgatgcgct gacgcctgcc gatcgctatc
     2101 tcgaactgtt cgaacatgtt cagtcggcga aaattttccc cgacagtaaa acctttcccg
     2161 actgcgcacc taaaatggac ccgctggata tcttaatccg ctaccgtaaa gtgcgccgtc
     2221 atcgtgattt tgacttgcgc aagtttgttg aaaaccactt ctggctgccg gaggtctact
     2281 ccagcgagta tgtatcggac ccgcaaaatt ccctgaaaga gcatatcgac cagctgtggc
     2341 cggtgctaac ccgcgaaccg caggatcaca ttccgtggtc ttctctgctg gcgctgccgc
     2401 agtcatatat tgtcccgggc ggccgtttta gcgaaaccta ctattgggat tcctatttca
     2461 ccatgctggg gctggcggaa agtggtcggg aagatttgct gaaatgcatg gccgataact
     2521 tcgcctggat gatcgaaaac tacggtcaca tccccaacgg caaccgcacc tattatttga
     2581 gccgctcgca accaccggtt tttgcgctga tggtggagtt gtttgaagaa gatggtgtac
     2641 gcggtgcgcg ccgctatctc gaccacctga aaatggaata tgccttctgg atggacggtg
     2701 cagaatcgtt gatccctaat caggcctatc gccatgttgt gcggatgccg gacggatcgc
     2761 tgctcaaccg ttactgggac gatcgcgaca caccgcgtga cgaatcctgg cttgaggacg
     2821 ttgaaaccgc gaaacattct ggtcgcccgc ccaacgaggt gtaccgcgat ttacgcgcgg
     2881 gggcggcctc cggttgggat tactcttccc gctggctgcg tgatactggt cgtctggcga
     2941 gcattcgtac cacccagttc atccccatcg atctgaatgc cttcctgttt aaactggaga
     3001 gcgccatcgc caacatctcg gcgctgaaag gcgagaaaga gacagaagca ctgttccgcc
     3061 agaaggcctg tgcccgtcgc gatgcggtaa accgttacct ctgggatgat gaaaacggta
     3121 tctaccgcga ttacgactgg cgacgcgagc aactggcgct gttttccgct gccgccattg
     3181 tgccgctcta tgtcggtatg gcgaaccatg aacaggccga tcgtctggca aacgccgtgc
     3241 gcagccgatt actgacacct ggcgggattc tggcaagcga gtacgaaacc ggtgaacagt
     3301 gggataaacc caacggctgg gcaccgttac aatggatggc gattcaggga tttaaaatgt
     3361 atggcgatga ccttctgggt gatgaaatcg cgcgcagctg gctgaagacg gtgaatcagt
     3421 tctatctgga acagcacaaa ctgatcgaga aataccatat tgccgatggt gttccccgcg
     3481 aaggcggcgg tggcgagtat ccgttgcagg atgggtttgg ctggactaac ggtgtggtgc
     3541 gccgtttaat tggtttgtac ggtgaaccat aatattttta cagccagccg ctaacttcct
     3601 gctggctgta aaattatcct cttcaggagg agatatttaa catcattgcc gcctgggtgc
     3661 gatttttcac ttccagacgg cgatacaggg attccagatg cgcttttacc gttccggtac
     3721 tgatattcag cgccctgccg atctccttat ttgattcgcc cgccgctaac atggttaaaa
     3781 tctcccgctg gcgggcgctt aacgatttga gatctttaat gtccttttcc ggcgtcgtcc
     3841 gccagtctcc aggcagaaac atcatcccca tcgccgcact atttaccgcc aacgcaaatg
     3901 tctcgacggt tgaatcacga ggcacaatgg ccagcacatt aaaatggata acttcctgta
     3961 accaccgttt attgcaatcc gtcgccgtaa ttaacacctt aacctcagga aattgcacca
     4021 cggttttttg cagcaaccag taacaaaact caccatcctg atcgccatcg agcatcacta
     4081 aggcttcagg gtaactttcc agcttttgcc ataactcgtc tgcctgactg gccccctgaa
     4141 tactcactcc tggaatacgt tgctgtaaac tgattttcat tccatgaata aatattgact
     4201 gcctgtcaaa catgactatt tgcataactg aatctccacc tgaatacgtt aaaaagactt
     4261 aagtagtgga agggtattac ccgcgagaaa aaataagaat tcgccatttg gcggtggcca
     4321 ttctacagag atgacgcgta gaaaatagtt accgatataa atagttacag ctaaacgcct
     4381 gaaattacat gtcgagggca ctatttaaaa caattttgag gatttcctta tattagtggt
     4441 tagtacgcat gcaattaaaa atgaaattcc gcgaccacaa gccaaaataa caaatggcaa
     4501 tgagacaaaa ataagcacaa atagccaata cgtcctctat tcactttaaa gggaatcgct
     4561 gaaaaatacg ctctgtttaa ggggattcac ctttctaaga aagctattcc tcccttttcc
     4621 tactgagaaa tcgccacatt cgtcatgaca acattgtgaa acccggcttt agatgttaga
     4681 aaaaacaata aacaatgcga tatgcgcgtt actgtttcgc tgtgaacaac aatcggtcaa
     4741 agaaatggat aaaattcacg caatgcagtt gttcatcaaa gtcgcggagc tggaaagttt
     4801 ttcccgcgcg gcggatttct ttgctttgcc aaagggaagt gtttcgcgcc agatacaggc
     4861 actggaacat caacttggca cccagcttct ccagcgcact acgcgacggg tcaaactcac
     4921 gccagaaggc atgacctatt atcaacgggc aaaagatgtg ttgagtaatc tcagcgaact
     4981 ggacggtctg tttcaacagg atgccaccag tatcagcggt aaattacgca tcgacatccc
     5041 gccaggaatc gcgaaaagcc tgttactgcc gcgcttgtcg gaatttctct atctgcatcc
     5101 gggaattgag ctggaactga gtagccatga ccgtccggta gatattcttc atgatggttt
     5161 tgattgcgtg atacgcactg gcgcattacc ggaagatggc gttatcgccc gttctctcgg
     5221 caaactgacc atggtcaact gtgccagtcc acactatctg acgcgctttg gttatccgca
     5281 aagccccgat gatctgactt cacacgcaat agtgcgttat acaccgcacc tgggtgtaca
     5341 tccgttaggt tttgaggttg ccagcgttaa tggcgtccag tggtttaagt ctggcggcat
     5401 gttgacggta aacagtcgcg aaaactatct cgccgccggt cttgccggtc tggggattat
     5461 tcagatcccg cgcattgccg tgcgcgaagc cctgcgtgcc gggcggctta ttgaagtgtt
     5521 gcctggctac cgtgccgagc cgctctccct ttcactggtt tatccgcagc gtcgggagct
     5581 ttcccggcgt gtaaacctgt ttatgcagtg gctggctggc gtaatgaaag agcacctgga
     5641 ctgaccgact atacttttta agaatgacca cgacagaagg acaaaagagc ggatgacgca
     5701 ggaaaacgag atcaaacgtc ccacccagga tctggagcac gagccgatta agcagttgga
     5761 taatagcgag aaaggcggca aagttagcca ggcgctggaa accgtcacca ctactgccga
     5821 gaaagtccaa cgccaaccgg ttattgcgca cctgattcgc gcaacagagc gctttaacga
     5881 tcggctgggt aaccagtttg gtgcggctat cacctatttc tcatttttgt cgatgatccc
     5941 gattttgatg gtatcgtttg ccgcaggggg ctttgtgctg gcctcccatc cgatgttgct
     6001 acaggatatc ttcgacaaaa ttctgcaaaa catcagcgat ccgacgctag ccgccacgtt
     6061 gaaaaacacc atcaacaccg ccgttcagca gcgtactacc gtagggcttg tcggcctggc
     6121 ggtggcgctt tattccggca tcaactggat gggtaacctg cgtgaagcga ttcgcgccca
     6181 gtcacgcgat gtctgggaac gctcgccgca agatcaggag aagttctggg taaaatatct
     6241 gcgtgatttt atttcgctga ttggtttgtt gattgcgctg attgtgacgc tttcgatcac
     6301 ctcggttgcc ggttcggcgc agcaaatgat tattagcgcc ctgcacctga acagcattga
     6361 gtggctgaaa ccgacgtggc ggttgattgg cctggcgatt tccatcttcg ccaactatct
     6421 gcttttcttt tggatcttct ggcgcttgcc gcgccaccgt ccgcgcaaaa aagccctgat
     6481 tcgcggaaca tttctggctg cgattggttt tgaagtgatt aaaatcgtga tgacctacac
     6541 cctgccatcg ctgatgaaat ccccctctgg cgcagcattt ggttccgtgc tgggactgat
     6601 ggcatttttc tacttcttcg cccgtttgac gctgttttgc gcggcgtgga ttgccaccgc
     6661 cgaatataaa gacgacccgc gaatgccggg gaaaacgcag ccttaaaata ggccggatgc
     6721 ggcgtacgaa tttattcggc ctggctcccc gtaggccgga taagatgcgc cagcatcgca
     6781 tccggctata atgcgcacat aacctcttga aactctttcc cagagcctct ttagccatct
     6841 attcaggagc aaacaatttc ataccaactc ataaccccag catataaatc cagttggtaa
     6901 cttttattta acctgaaacc agttttatcc actatttata aaattatgtg aagcatttca
     6961 tagaagaaaa atcactggcc taaacattat cccctttttg cctggttttt gaccatttcc
     7021 gcgatttgtt acacattgaa atatcacttt tgctgtgcgt aatatggcta ttcgttagcc
     7081 aaaaaataag aaaagattat gcaagcaaca gccacaacac tcgaccacga gcaagaatac
     7141 acgccgatca actcgcgtaa taaagtcctt gtcgcctctc tcattggcac ggccattgag
     7201 ttcttcgact tttacattta cgccactgcg gccgttattg tgtttccgca tatcttcttc
     7261 ccgcagggcg atcctacggc agcaacgcta cagtcgctcg ccacctttgc catcgccttc
     7321 gtcgcgcgcc ccattggctc tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa
     7381 gcgacgctgg tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat tggcctgctg
     7441 ccaggctatg ccacgattgg tattttcgcc ccgctgctgc tggcgctggc tcgatttggt
     7501 cagggtctgg gcttaggtgg tgaatggggc ggcgcggctc tgctggcgac tgaaaacgcc
     7561 ccaccgcgca aacgtgcatt gtacggctcc ttcccgcagc tgggcgcacc gattggcttc
     7621 ttctttgcca acggcacttt cttgctgctt tcctggctac tgaccgacga gcagtttatg
     7681 agctggggct ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat cggcctgtat
     7741 gttcgcgtgt cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag
     7801 gtgaagatcc cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt actgggtacg
     7861 ttcattatgc tggcaaccta tacgctgttt tacatcatga cggtctactc tatgaccttt
     7921 agtaccgccg ccgcgcccgt tgggcttggc ctgccgcgta acgaagtgtt gtggatgctg
     7981 atgatggcag ttattggttt tggcgtgatg gtgccagtcg ccggattact ggctgatgcc
     8041 tttggtcgcc gtaaaagcat ggtaatcatc accacgctga tcatcctgtt cgcgctgttc
     8101 gcctttaacc cgttgctcgg ttctggcaac ccgattctgg tttttgcctt cctgctgctg
     8161 gggttaagtc tgatggggct gaccttcggt ccgatgggcg cgctgttacc agagctgttc
     8221 ccgacagaag tgcgttacac cggagcatcg ttctcttaca acgtagcgtc gattctcggg
     8281 gcttccgttg cgccgtatat cgcagcctgg ttgcagacta actacgggtt aggtgcggtg
     8341 gggttatatc tggcggcgat ggctggcttg acgttaatcg ccctgctgct gacccatgag
     8401 acgcgacatc agtcgttgta ataagagtga aaagcctgat gcgctacgct tatcaggcca
     8461 gaatttccat acaatatatt gaattagcaa ggatttgtaa gccggaggcg ttcacgccgc
     8521 atccggcatg aacaaagcac actttgccaa caatctaaat ctgtttaatt gccggatgtc
     8581 agacatccgg caattatcat cacttcttca tctgcgacaa aattgtcctg cactgattag
     8641 cctccccttc ggaaggtgag atcagcgcca gtaacgccgc cgccggtgtt accagcgttg
     8701 ccagtgccgc cgcaacggct ccgcgagcaa tcaacggccc ggctttcacc ccagcctgcg
     8761 gatttttaaa cgtcccacgt acatacagcg gcgaacgcaa tgtgataatc cgaatccctt
     8821 tactctccgg atcaatagtc aaatccagct gttccgaagc aaaacttgcc gtaccggtaa
     8881 cgttaatcaa cgcgttctca gtatcgaaag caaaaatctg cgggcgcgcc acgccgttgg
     8941 caatatccag attcgccgcc gcacagttca cccgcacctc atcatcacca aatatcgcac
     9001 cgacaatgta gttaccgaca ttcagcccga caatctccat caggttgcgg ctcaccagcc
     9061 cgtcattcat cagcagtttc aggtgccgtt actgttgcct aaaagcgccg ccaccgagtt
     9121 accgctaccg cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc
     9181 tacatcgggc atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccttg
     9241 catcggtttt ttatcacctt ccagatgaat attcgccgca atgctgccgc ctgccatgcc
     9301 aaatttcagc ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc
     9361 gctaatcggc aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc
     9421 catgacgtcc catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg
     9481 aacgcttttt tcacccttct tctgttcaga ccgtttcgac ttttcagctc ctttcccgga
     9541 atcaacgcca attaacggcc ctaaatctgc cagccgcaat tgccgcgact caacatcacc
     9601 ttccagtttt ggtcgtggct tgccggtggt gtagaccaga gaaccgtgga tatcgctatc
     9661 gccaattcgc ccattaaaac cgcgataatc aaagaccgac gatttttcag tgtcgatttt
     9721 cgctaccagc cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag
     9781 ttcatagaga tcacccagcg aatcgccaga aaatttaagc cgtaaatcga caccacccat
     9841 cttcattggg tcattcacaa cgccatcaaa agcaacacgg gtgttaccag agcgaaaatc
     9901 agcctgtaca ggaaatggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc
     9961 cgtaccagtg agcggctctc cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc
    10021 gcccaccttt tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg
    10081 cttgcctaac ggatcgacaa aaatttctag atccgctttg cttactttgt catcaatggc
    10141 gatccgccct tgatcgaaca gaatattatc cagccgaaac gaccatgccg acggctttgc
    10201 attcgtgtct ttgttgtcat cgctggcgag attaaatgtc cagttgttgt tcttttctga
    10261 gaggcgaatc aggcgtgcgt cgggcttttc gagcttgatc cacggcaacc agaccgtttt
    10321 ggtcagcagc gccagcgggg ccagcgtcgc ttctactcgc ggcaaatgca ccattgtgac
    10381 ttcgggaata tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg
    10441 cacccagctg cgccagccag tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc
    10501 acggatagcg aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag
    10561 tcgattccag tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc
    10621 ccctgaaatc gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt
    10681 cctgtgccag tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag
    10741 aaccgccgta ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc
    10801 gaatgcgggc gagttctgaa catcacgcca ctcttccggc gtttctacgc cctcgacaat
    10861 caccccgcga caatagcgat tcatgagatg taaaagctga gaaaagagtg tgcggccttc
    10921 cggcgactga cgcagcatca cgaacagctc tcgcgcgatt ttgatgtagt cataacgcac
    10981 ttcacttagc gcagagaaat ttgccatccc ggtgccaaaa tcatccagcc acagcgggcc
    11041 aaattcacac atcgaggcaa aggttgaatc tttcggcaga cggatatgct ccaccagttc
    11101 gaaacgcagc cagggaagac gctcaatttg gcgcaggatt tttggttgct gacgcagggc
    11161 gatgagcgta gggccatcaa tattgaccga tgccagcagg tcgtgctcta taaagaagtc
    11221 ggctttttgc gccagcaaat caatctgctc tttcacaacc tccatccgat ggctaacggt
    11281 gatttcagta aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac
    11341 caccgttaat agctccacgg ccattaaccg tccgcatgtt tgatagatcg gctgccaggt
    11401 gtaagcacgc tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc
    11461 agggttgctt attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt
    11521 gatgctgccc ggactcgaca aagattatcg gcgcgcaagg gcagaacttt accgcctggt
    11581 cggggtaaat ttccccactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa
    11641 ataatggaac actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct
    11701 aattttttac agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc
    11761 atgattgagc tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg
    11821 aacacttccg tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta
    11881 acggcgctgg gaacggacag ttttagccag cagatgctgg acgcctggca cggcgagaac
    11941 gttgatactt ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa
    12001 accgacagca ccggcgaacg tacgttctac tactggcgta acgaagccgc cgccaaattc
    12061 tggctggaga gtgagcaatc cgcggcgatt tgcgaagagc tggcgaattt cgattatctc
    12121 tacctgagcg ggattagcct ggcgatctta agccagacca gccgcgaaaa gctgctttcc
    12181 ctgctgcgcg aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg
    12241 cgcctgtggg ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg
    12301 gatatcgcct tcctgacgct ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa
    12361 gacgtcattg cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg
    12421 gattcttgcc tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtaaaactg
    12481 ccgaaagaaa aagtgatcga taccaccgca gctggcgact ctttcagtgc cggttatctg
    12541 gcggtacgtt tgacgggcgg cagtgcggaa gacgcagcga aacgcgggca cctgaccgca
    12601 agtaccgtta ttcagtatcg cggcgcgatt atcccgcgtg aagcgatgcc agcgtaaaag
    12661 cagcatatgc aaaaggctgg atagagtgtt acccccctat ccagctcatc cataaatacc
    12721 tgatggttgt gctaactcat caccatcagg cattactgta caggtggaat atccgtcact
    12781 tcaggatgta cgtcatccgt ggcgacagat gtggcggcag ccgcagtcga tggggccatg
    12841 atttgatccc aggccgcctg caacgctttc atgttaaatt ccggctcgcc tttcggctgc
    12901 agcagtatta acgccatatc attcgacaac tgctgacgca gatcctgatt taacatctcc
    12961 accgtcaggc tgttaaggaa atcctgacgc agtttctgat attgctccgg cgcgatatcg
    13021 acgacctgat tttgcaacga acgcatccgc tgccccatca gaatatcggt atcagctcgc
    13081 gcataggcgg caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac
    13141 tcttcttccg gcagaccttt atcgcgaacc ttcgccagtt cacgcgccac cagattaagg
    13201 ttgctgttca gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac
    13261 agcacacgac agtcaaatcc aagaccgatg tctttactgt tactggcgct taacgcttgc
    13321 tgaacatgcc agaacagcgc ctcacgggcc aggtccgcac gccagtagcg cagcagtgcg
    13381 gctgattcac gaatcggctg ccacggcgta tcccacatga ttgataaccg gtcctgacgc
    13441 accgcgtcag tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga
    13501 gccggtgttt cacgtttgcc tttcagttcg ccaaacgttt tattgatttg gtcgacgacc
    13561 gagcgcgcat ccacgtttcc caccaccagc agcgtcattg catccggggt ataccatttc
    13621 tgatagaaat ctttaatctt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg
    13681 tgacctaaca aggttgatcc tttcagacga tagcgccacc agccctcttt agtatcggca
    13741 ggccaggttg ccaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggtgtgatg
    13801 gtcagtttgc cagtggcatt tgccagataa gagagcgctt ctttcagcaa gtcgttacgg
    13861 ttattgggca aactcagatt aaacagcgtg gtgtcataag agacaattac tggcggcatc
    13921 gggcgtttag ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc ttcaaggcca
    13981 ccgctttgcg ttaacgcaat acgagggatg gcgtggctgt aaccgctctg ttgtgtactt
    14041 tcggcgagcg aaccggtatt aaccagcagg cgaatttcaa cacgatcgct aggacgctgg
    14101 ggcgtggtca gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga
    14161 tcaggctgga gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc
    14221 gctaaaagtc gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa
    14281 aaaaactgcc cgttgtcggt cagtctaaat gacgttaaaa acacttcgtg ttagaccgca
    14341 agaacatgaa aatgtcacgg aagaagtgaa ataaacctga actcacccag gtggtaagtt
    14401 caggcacagg ggtcaattat gcgcaaacac ccgcactcgg ggaagggagt gcgggcataa
    14461 gtgatgagat taagaggata attcgtgcgt tttgccatcc ggcgcacgat tattcagcac
    14521 atcgtccagt tttttgtggt ccagttcttt cacccactta gcaacgacaa tggtcgctac
    14581 gccgttaccg accaggttag tcagcgcacg agcttctgac ataaagcggt cgataccaag
    14641 gatcagcgcc agacctgcta ccggcaaatg gcccaccgca gagagcgtcg ccgccagcac
    14701 gataaagcca ctgcccgtta ccccggcagc ccctttggaa gagagcaaca acacaattaa
    14761 tagcgtaatt tggtggacga tatccatctg actgttagtc gcctgggcga taaacaccgc
    14821 cgccattgtc aggtatatcg atgtgccatc aaggttaaac gagtagcctg tcgggatgac
    14881 cagccccacc accgatttcc ggcagccgag tttctccatc ttgtcgagca tacgcggcag
    14941 cgccgactcg gaagaagaag tgcccagtac aatcagcagc tcttcacgga tgtagcggat
    15001 aaatttgaag atactgaaac cagtcgcttt agcgattgaa cccaatacca gcaccacaaa
    15061 caggatacag gtgatgtaga aacagataat cagctgcccc agttgcacca gtgtgccgac
    15121 gccgtattta ccgatggtaa acgccattgc cccgaacgca ccaataggtg ccagacgcat
    15181 gatcatattg atgatgccga agatgacctg cgagaaactt tcgatgacgt taaaaatcag
    15241 ttggcctttg ctgcccagac ggtggagcgc aaaaccaaac agtacggcaa acagcagcac
    15301 ctgcagaatg ttaccgctgg caaatgcgcc aatgacgctc gccgggatga catccataat
    15361 gaaggcgaca atgccctggt ctttcgcctg atcggcgtaa accgctaccg ctttcgcatc
    15421 aagcgttgcc ggatcgacgt tcattccggc accaggctgc acgacgttaa cgatgataag
    15481 accaataatc agcgcgatgg tactgacaat ttcaaagtaa agcagtgcga ctgcgccggt
    15541 acgaccaacc gccttcatgc tttccatgcc cgcaatgccc gttacgacgg tacaaaagat
    15601 gacaggagcg atgatcatct taatgagctt aacgaacccg tcgccaagcg gtttcatttg
    15661 ctcgcctatt tcagggtaga aatggccaag gagaatacca atggctatcg ctgtcaggac
    15721 ctgaaagtaa aggcttttaa acagagaggt tttcataggg tgtcctttag taaaaccaca
    15781 ggtcttgtaa ggttatgggg tacctgcggc cctaaaataa cacccagaca acatcacaga
    15841 aatgtacctg gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa
    15901 ccagcacttt ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc
    15961 tcttcgaaga tttcaatagg gagtgggcga gcaaaaagga agccctgggc aataccaacg
    16021 cccgcttttg ccagccagtc gcgttgtgct tcagtctcca cgccttcggc aatcatttgt
    16081 aagttcaggc tttgcgccaa catgataatt gcagcaatca tgctgctatc ttccggcaag
    16141 ccttcaacaa acattttgtc gattttcagt acgtcgattg gcaacgattt catatgctgc
    16201 agctgacgca gccctgcgta gcccatgccg aaatcatcca gcgccacccg aactccggca
    16261 ttgcgcagcg gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct
    16321 gtcacttcca gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata
    16381 tccgctacca tattcgggtg catcagttgt agcgctgaga ggtttaccga caagggcagc
    16441 ataatgccgc gctcttgcca ggcagcaagc agtcgacaag actcttccag cacccagtga
    16501 ccaacggtaa ccatcagccc acagcactca atgcgatcga ttaagccatc cggcagatcc
    16561 caactgccat ccggttgctg gatacgcagt aacacttccg cactgaccag tttaccgctg
    16621 gtcatctcga cctgtggctg taaccaaatc gcaaattgat gattttccag tgcattaagg
    16681 atatcgctct cttccgtcaa ccgctgctgg gcggcttcca tctgctgcgg atcaaagaac
    16741 tgaatctgat ttttgccttt atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa
    16801 agctgttcgg cggtgagatc gccgtagaac atcgccacgc caatgctaca gtgcggacgg
    16861 agttgaatac gttcaatcgg caggcgctcg ctcatgatag tgagcacttg ctgacctaat
    16921 gtgatagcgt gccacggttc ctgtacaccg ttggcaatga cagcaaagtc ataaccgcta
    16981 atctgcgcga ggatcatgcg tggcgacagt accgatttga gtttttccac cagcgtcagc
    17041 agcagaattt ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag ggtttcacag
    17101 gtgatgatca tcagcgcggt ggtttgttta cgcgcgacaa cctgctccag catctccatc
    17161 agcaaggctt tgttcggcaa atccgacacc gggaagcgca tcgcgttttc gttctgttct
    17221 tcataatggc gctgcagcaa ttgctggttg aggttgtaac tgcgcaccag cataccgatt
    17281 tcatcgtcct gatgcagacg tggtaatgcc agttggtgac caacaagctc ctgggctggg
    17341 atggcgttaa gttcgcgagc aatattgcgt aacggatgta aaatcagtcg attaatacac
    17401 cagctgatgg cgacggtcag gataagcgac aaaagtaagt aaatggtcac taacgttgag
    17461 agggtgctca tcacgaactt atacatacgg aaggaatccg cctgtagcac cagataggca
    17521 attggctgcg gatttgccgg acgttcgagc gagtagacgc ccagcgagat ttgaaccggt
    17581 agctcaaaca ggcgagtaac cattaccggc accggacgct ctggaataaa acttttacgc
    17641 agcgcctgga actggttggg cagcaccaca tcggcacggc tgaccacgcc tgccggttta
    17701 atgctggcaa gaatagcttc cgcttcggga atatcgcctt tcaaaatagc tgaagataag
    17761 ggttcgcgga cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgc
    17821 tggaccagat ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct
    17881 gccaccattg ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc
    17941 ggaaatccca gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat
    18001 ctgaaaactt accagtcggc gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc
    18061 tgccatccgg cggcggaata acgtacgtag agcaggaaat ggctgggtgc gtaatctttc
    18121 gcctgctgga tatcaattgc cgtgccgaca aaccagttgg aagtaacacg tcgttcaagt
    18181 aatgcccgcg ccgtatagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca
    18241 gcttcttcct gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg
    18301 gtgcgtgaat gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc
    18361 tcccgccaca tcaccggtat ggcaaacgac aggtattcct gcgggctgta gtaaccgccc
    18421 tgaccgagtg agtagccact caggtctttg tcgtaatgcc agatcatgtt gttcaggccg
    18481 attgtgacgc ggcgattgtt ctggttaatg accttatagt aatagcccgt catccagcgc
    18541 acacgccagt tatcttcgac atttttaccg gttaactggt cgccactaag cgatgcccag
    18601 acgccgtttg cttcaccttt atcgtagctc aggcttagcc ccacgccgtc ggcacgcacg
    18661 ccgccccatt ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa
    18721 gaactggaga tgggccgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg
    18781 ctgtaactga tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtgccgata
    18841 tcccagctcc aggtgtcgtt gcgccaaccg accgccacgc tggcaccgga atccgactgg
    18901 ctgcggttgc cgctacagtc ctgtaatgta caggtgcccc agttgtcatc ccatttacca
    18961 tcggcattag tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc
    19021 cgcccgtcag aatacggcgc atccacctgc aacatggtag tgtgcgcttt cagatcggag
    19081 taaccaccgg tgccgctcga accccagtaa tcatgctcaa gggtgacgtt aagatcctgc
    19141 tggcgataga ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg
    19201 tcattgcggg tcagtcgggt aaaggtgtcg ttatccagcg gacgcgtcgt ggtcacaccg
    19261 gatgcaacca tcgcgtcttt gtaggtttcc agcgcctgcg ttggatcgcc cgcctgcgct
    19321 tcaaacttcg caccatcacg cagtaccatc gcactttcca tcgacggcgg ctgagatttt
    19381 gccagcggga tcaacttatt aaacgtctgc tgcgccgctg cggtatcgcc aagctgcgcc
    19441 tgtgccagcg ccacgcgccg ctgagtgttc agcgaggcgt tatcggtggc aggcagcttc
    19501 gccagctggc tacgtgccgc cgctttgtca ccggcagcaa tatccacttc cgtcagacca
    19561 agaatggcat cggcgttagt tggctcccgc gtcaggacat tctgatatgc agcgcgagcg
    19621 gcggtgtaat cacgtcgttg ttgcgcccag tcagccagcg tgaggtctat gcgcgtggaa
    19681 ggtggttgct ggcgcagcat cgcttccgct tcagcttctt tgccgctttc tcgcaggcga
    19741 ttagcggttt ccaacacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg
    19801 ctccactgcg cgcgcggcag gctgttgata tgcgccagcg ccgctctgtc ctggtcatga
    19861 ccagagagat acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc
    19921 agattacgca ttaacgtatc ggcctggctg cgttgtccgg cctggctgcg ttgtccggcc
    19981 tgccagagat cctgtgaaag tcggtaagta atccatacac tgccggggtc cagcgccagt
    20041 cgttgccgct gaagtgctgc cgcctgcgcc catttcccct ggttttccag tgcctctgcc
    20101 tgctgtgcca gacggtcgtt ttgcaggctg cgttcgatat catcaatgct gcgccgctgg
    20161 ctggcagaga gcgaggcgat atacgcttcg gctttttctg gcgactgctg gcggtaaata
    20221 ttcgccagcc cgcgcacggc gttagtgttg ccgctgtcca tacgcaaggt ctgctggtaa
    20281 tagcgttcgg cggcgggata atctttgcgc gccatcgcca catcgcccag cccaagcact
    20341 gcatagctgt cggtgttatc gacattacgt gcctgctgga acagccgttc tgcccggtca
    20401 ggattattgg ctttcagcgc ggcatcgccc tgctggatcg ccagccagta gcggtttact
    20461 ttcagcagac tgttccattt gtcgttattg ctgttgtgcg gatccagcgc gagggctttt
    20521 tccagattcg ccaccgcatt agcgcgatcg cctttctgag aatacgcctg gcccagcgcc
    20581 cccagagcct cactgtcttt cgggttcgcc cgcaccgcct gttgtagttc gggaatggct
    20641 ttacccgcca tgccagagtc caccgccgct aaaccttgcg cacgagcgcg gaaagcaggg
    20701 tcggccagct gtttttgctg ttctgccagt tgcgattgtg cagcagctac gctatcgcca
    20761 tcgctaaaga tcgagagata ttttttcagt gcctgaacgc tggcatcgct gacaggcatg
    20821 tctttaatct gcccgtacca gattttagag gccccttcgc gcccggcgtt cgatttcgcc
    20881 atctgttcca ggacggcaaa accttcatca cggcgatcgc tactaaacag taatagcgcc
    20941 agattgtttt gcaggcccgt gttgcccggc gtatccgcat taatgcgttt tagctgatta
    21001 atcgcttcgc cacgtcgggc cggaattttc gccaccgtac tccagtactc gacagcaatg
    21061 tcaccttccg gcggcgcacc gttgaacagt ttgttgtaac tcgccacagc ttcttccgca
    21121 tgaccagtcg tcgcctgcaa tcttgcctgt tgcagtgcct gacgaccatc cgacgtggaa
    21181 agcagcatcg tagtccgtga cgatttatac gcatttgaac tcggcgctaa ctgcgacagc
    21241 cgatcgagct gtttttgtgc gccatcaata tcgccctgac gtaacagaga acgaaaacgg
    21301 gcagcaacga cgtccgggtt attcggatca ataagttcca gacgatacaa cgactgttgc
    21361 accaggtctt cacgatgggt cgcttcgcct aaccgaactt gctccagcaa ctgttgctga
    21421 gcggttggtg ctgcctcgac catcggcatg acggccagac cgagggaaag cgtgaatatg
    21481 tttagtgtga atttgcgcat tcctggcccc agtcaggtaa taactcacct tttgtcgaga
    21541 agcggaaacg gtgttgatcc cagccttgtc caaacagggt cagcacatag ttgtaatagg
    21601 catcgctgcc aggaaagtta tcggccacgc gctggcgctg aacggcctgc gcatcgcggt
    21661 tttgtaaaaa gggcagcatg gcggcagaaa aaccgaccgg tcctttaccc tgcgctttcc
    21721 ccgtagccac atccactttt tccggcggat aaccgttttt ctcagtgaat gtcgccatcg
    21781 gtttaaaccg gttgagcatc cgcgcttttt gcggatcgct gtcaggcatc atgcctaccc
    21841 acatgtaaac gcggatagcg tcgtagctgc tgatcaatgt tttttcggct tttagctgcc
    21901 agcctttgtc tttctcatag cgcacccagt ctggcgaaaa gcctttcggg gcggtttcca
    21961 gcaataaacg ttgattggtt tcgcgcagcg tggtccacgg cgcgccaaag cgggtgaaat
    22021 actgcgccag cgtcggcggc aggtagctgg ggttaaaacg ccagctgtta tcctctgcaa
    22081 atcccacttt gcccggtaac aacatggagc ccagcccagg caccgtcacc acttcctccc
    22141 gcgcgatacg ttttagcaat gcgctgccga tgtcggtata acgctgctct ttccacaaac
    22201 gccccgcctc cagcaacgcc caggccatcc agacatcacc atcggaggcc gaattgctgt
    22261 ccagcacttc ccacttactg ttctctttct tgccccacag ccaggcgggc aaatgttctt
    22321 ttaaagaacc ctgtgcgaga ttgttctgcg tccagtcgag aagattatcg aaagctgcac
    22381 ggtcgttagc cgccagggca aagaacatgc cgtaactttg cccttcggag gtggtgattt
    22441 tgcgcgcgtc gctggggtcg atgacgcgcc cttcctgact gatgtaatcc tttttaaact
    22501 gctcccaggc aggccaggta caggctgcct gaacactaaa ggcagccagc agcagcatcg
    22561 tcacgagtcc actacgcaac acattcatct tcaattactc gttatccggg ttaagacgac
    22621 gacggctgat aatacgcagc agacgccaca gtacccatgc cagcagcacg acgctgattg
    22681 ccgccagcac cgccagcaga atcggatggt ttgccagcgc ataccataag cgttcgaacc
    22741 acggcagatg gccaacgtaa tagacgtcgc caacacgcag gctattgata ccggactcgc
    22801 ggatcaccgc gaccgaaccg aacatggtgg cgcgtttgcc gctatcgttc accgcatcgt
    22861 taagcatttc ataaccgcgt gggctatctg ccagcagcgc aatcacgcta cgctggtcgt
    22921 tatacggcga ctggaagcca atcaccgctg ccatcgcacc tgaagaggtc agcgttgact
    22981 gagtttctgc cgcgcgatcg ctctcgtccg gtacaatgcc ggggaacggg gtctggcgca
    23041 tcggcgtttt cacccagctt tcggtcgcct gcaccaacag gtcgatctgc ttgtcgtctt
    23101 tcagtttgtc cgggatacca ccgacgatca tgatgtcggc atctttcccc tgaatagtgc
    23161 tgccatcatc ggtcaccgtc aggttaatcg ccgggaagcc cgtctgcgca ccgataaagc
    23221 caacggtatt cagcaaggtt tccatctgtg cttcgttagg cgctttcggc atcacggtga
    23281 tggtttgtga cagatccgcc atccggctga acgggaagcc cgcgttagca aaggcgcgta
    23341 gatccggcat cgggatgaag tggtaatact tcgagaagtc gatggtggaa tcatcaccaa
    23401 tcaccacatg attctgcacc ggctggaagg taatgcagtt atccaccgaa ccgcccggca
    23461 tcgggttcat atactcaaag tcaaagcgca gctggttggt cgcgcccagt ttcagcgccg
    23521 gaatagagac atccgttttg ccatccagca aaccttgtaa taccggaata cgcagcagca
    23581 ggcggttcgc ctcctgttta ctgctcaggt tgaaggattg caggaactgg ttattcaggc
    23641 tgatatccat ccgtgagctg tctttcactg gcggcatggt gtagcgataa ttaatatcca
    23701 tatcaatgcc ggtactgcgc atcaggtaga gatccggtgg caggtttagc gaaacgttaa
    23761 tcgctgctgg ctcaagaccg ctggattgta actgttcttc ataggttttc agttcaccaa
    23821 aggtaaccgg acgatcggta cgcacccagt tcggcgcatc gtacggctta cgcggtagca
    23881 gcggtttcac ttcattcact accacgcttt caccacggaa cagaatgtta ccctgagcga
    23941 tacctttcgc tgcctgcaac aggtctttgt cgtcacgacc aaacaccacc agcagtttga
    24001 cataaggatt ctgcggatgg ttgatcattt caatcaccgg ggcttttacc gccggatgat
    24061 cgcgcaggaa gtccgggcgt ttgtcattgg tggcaaaaac gatggcattg cgatccggca
    24121 attggttata gagcaccggg aagttctgcc cacgccagcc agaacgcgaa ccaaaccacg
    24181 aggcgacaat ggcagatgct tgttgcagcc cgacatccgg cgcacccgca aagaccatcg
    24241 gcaaggtgtt agtacggtta tcacgcgggt caaagaatgg caccgggaag tgtgacaggt
    24301 cattcttcac attcagggtc tgataggtca gatccagtcc actgctccgc ccaacatcca
    24361 gccaaagcgt ggtgctggcc gggttttcgc acacgtcctg ataatgaccg acaaactcca
    24421 gccgcacacg gttgaagtcg gtaataaaca gtgggttaat tggcatttgt gccagcgttt
    24481 ttttacccaa ctgttctttg gtcactggca gcacgcccat cagttcatca ttgagataaa
    24541 cctttaactg cgactggaca ggcagtaatg atggcgatgg cgtgtattcg aggttgagca
    24601 tcgccttcgt caccacttca tcgctgcgca taccaaactc aatgctgccg ttcgggttaa
    24661 tgccacgtag caccatgctg cccggcggcg gcgcaatttg cgcaaaggtc agcttcacat
    24721 cacgggaagg accgttctgc gccacgactg gcgcatctgc gccctgcacg cccggcatca
    24781 cttgccccac ctgcggattt tgctccgcct gggcggctac agctggctca gcattgatca
    24841 gtggttgcgt tgctggcgtc gcctgcgtca tgaaagaggg gaacgcactc atccccagag
    24901 ccactgcaca aatccagaat atttttcttt tcatcgcgtt atcatcattg ttgagccaaa
    24961 gcctgatccg atggttgtgc cgtttcgctc cgctccgggc ggcgcggaat aaacgataca
    25021 acccaggaaa ccagagaagt cagcacacgg aatatgccct tcaccgaaga aggcgcaaat
    25081 tccgccagat ggcggtagcc acggaagccg agcttcagaa tatccagcag actttccagc
    25141 ggcttatctt ccgggtagct gtcctgccag agcgcccatg tatccgcacg ggcaaacgta
    25201 cactgcacaa aatcgatatg ttgctgggta gtgagcggca ttaatttcag cccaacttca
    25261 ttacccatca cacgcgccac ctgagccggg aagacgtatt cctgctgacc gcgtttaagc
    25321 aacagattca ctttctgccc ttccagaatc tgcgcctgac cgttgatctt gatccccaaa
    25381 ccaccgtcgg agaaatcctg aacggtacac gagaagaggt gaccatcttc gcgggcaatt
    25441 gctgcgggca tcgtcatctc cacgcggtgc gatcggcgta cctgtttgct ttctaccgat
    25501 actgcgactg cgccgccgag aataatcagg ttgtagaata cccacaccat actgacgacc
    25561 acggtgagca tctcggttgg cggaccatag aagtagcgcc agatcccaac cgctacgccc
    25621 accaggttga gcagaacaag gaagatgtag ggccgcgaga tcacccagtc gacgtactct
    25681 tcttccacca gtccaccttt ggcggtgacg ttaaatttgc ctttgtgcgg gttaatcagc
    25741 gccaccagcg tcggtggtgc gatataccac gccagcactg tttcgtagat ttcactccag
    25801 aaagagtggc gatatttgcc ctggatcttc gagttagtca ggctggcatg gatcatatgc
    25861 ggtagcacga acagggcgat catcaacgct ggcgcgtaga tgatgtaggc atgaaacagc
    25921 aggaacgcca gcggcgcagt caggaagatc agccgtggaa tgcccgacaa gaaatggaac
    25981 atggcgttga catagcacag ccgctgagca aacttcagcc ctttaccggt gagtgggtta
    26041 tcgagacgga agatttgcac catcccgcgc gcccagcgaa tacgctgacc gatatgcgcc
    26101 gagagacttt cggtcgccag ccccgctgcc tgcggaatac gcatatacgc tgaggtgtag
    26161 ccacgacggt gcagacgcag agaagtgtgc gcatcttcgg ttaccgtttc gacagcaata
    26221 ccgccaatct catccagcgg cttacggcga atcaccgcac atgaaccgca gaagaaagtg
    26281 gcatcccaca tatcattgcc gtcctgcacc agaccataga acagcgtgcc ttcgttcggc
    26341 gttttacgaa aacgccccag gttgcgttca aacggatccg gcgagaagaa gtggtgcggc
    26401 gtctgcatca tcgccagctg tttctctttc aggaaccagc ccatggtcat ttgcaggaac
    26461 gatcgcgttg gtacgtggtc gcagtcgaat atcgacacga actcgccttt ggcatatttc
    26521 agcgcattgt tgatgttgcc cgctttcgca tgttcatgag tggtacgggc gatatatttc
    26581 acccccacgt tttgcgcaaa ctggcgaaac tcttccctgc cgccgtcatc gaggatccag
    26641 atgttcagct tgtctttcgg ccagtcgata cccagcgaag cgtaaatggt atttttcacc
    26701 acgttgagat cttcgttgta agtcgggaca aagatatcca ccgacggcca cagcgacata
    26761 tctttcggca atggcaccgg ctgacgattc agcggccata cgacctggaa gtagccgagc
    26821 accagcacta tccacgcgta cgtttcggcg aacagcagaa taagcccgca caccaggctg
    26881 accggatcgt cccagtttaa cgtcgaggtg taacgccacc agatataacg gcaagaaacg
    26941 gtcagcgaca gcacaatcag catcagcgcc gagaagcgcc ccggcatccg ccgtacgatc
    27001 agcgctaccc cccacagcag catcaggaag ataaactgcg ctagcgggtt aaacggctga
    27061 gtaacgcaga ttaacgccag aatcagcgag aaggtgacga tgataccaag gatcaaccgc
    27121 cgcgccccgg cactcaaatg accgagctct tttttctcat caagatgctg tgttttatgg
    27181 ctaacgcgct caggcagctc gttcatccat tgatggtaac gtccacgaat attttgtaga
    27241 cctgaaaatg cccgcctgcg cggtttcggc gtttctttgc gcgatgcacc gatcagtaac
    27301 cagcatgttt gaataagata acggaccggg tccagcggac gcggacgcga ggcgttgata
    27361 tgcggataca ggtttttatg ttctgcgcga atacgctgcc agcgcgggtg ctccagcgga
    27421 ataaaaatcc aggccaggat catccagaaa cagccgagcg tcgcgctgaa agccgacgca
    27481 ccgtgacgac gataatcgcg ataacgccca ataagccgtg cgttgaccgg cgggataagc
    27541 aaccaccggg tcaggatact catgatgcac tcccgactgg cgttttcagc ccggaataat
    27601 tcaacaggca ccagttcgcc agcgtcagta tctcttcagc cgccagcgca tcactgcgat
    27661 attcaccgac tggttgctta gctgccagac attcagccat cgcttcatca cgatgaatga
    27721 gcatcggcag caatcggcgc tggctttgca accaaagctg gtaaatatcg tcctgaacct
    27781 ggctgccaat acggaagtca ttaatcaaaa tatgtgcacc atccggcagc gcttgctgat
    27841 gcagtcggat atggcagttg gcatccacat tgacgattgc cagcgagtga tcgcacaaac
    27901 tcagcagctg gtgggttatc tgcgaggcat cacgcggtaa gtcaattaaa atccactggt
    27961 aacgcccgct ggcttttagt tgctgtaagc cggtgcaaat atcgctcagc cgggtttgcc
    28021 agtgctgtgg attttcttgt tcttcaatcg ataactgacc aaaaggcagc aaatcgagct
    28081 gcgaggtata gcgcaacccg gcgtcacgcc agtcctggcc atccagcata gctctggccc
    28141 aaccctgacg gtgggtaaaa tcaacgttaa atgacaggcg caacaagttg tccgggcagg
    28201 catcgaccac caggacattt tctcccaaca tttgtaatga ccaggctaat gcggcggtga
    28261 tggttgttgt ccccacgcct ccccgaaccc cctgcaatcc cagtacggcc attcaccaat
    28321 cccctacttt tgttgcgcaa actctgccag caacggccag cgttttaatg ccgcggccaa
    28381 ctgttcgcgt tgggaaatat cggcataatc aatatcaggc agtgaaaatg cctgctttaa
    28441 cgccaaaata tcattctgga agatatagcc tatcgcggga tcaggcagag tatctggttc
    28501 attgttattc attgttattc atttttcttg atccctatga aataaccgta ccgttcatga
    28561 gacaattcta actgtgagca tttcgtcggt ttattgttta cggcgagacg ggaatgtgct
    28621 cactttttga ctttccgtta cgcaacagaa aaagttaaaa acgaaaagac taaaatcaca
    28681 tgtcttgtga cctgctaaat tgattcacat gctaaatctg ataagtttta atttcaatgg
    28741 taggtttatt tcttagcttt cgctagtaaa ctgataatca gacaaaatgt tgacatgagg
    28801 gacattgtgg accctgtatt ctctatcggt atctcatcat tatgggatga gctgcgacat
    28861 atgccagcag gcggcgtctg gtggtttaac gtcgatcgcc atgaagatgc tatcagtctg
    28921 gcgaatcaaa caattgcatc ccaggctgaa accgcacacg tcgcggtcat tagcatggac
    28981 agcgatccag cgaaaatctt tcaattagat gattctcaag ggccgggaaa aataacatta
    29041 ttttcaatgc taaatcatga aaaaggtcta tactatttgg cccgtgattt gcagtgttct
    29101 attgatcccc ataattacct ttttattctt gtttgcgcaa ataacgcatg gcaaaacatt
    29161 cctgccgagc ggcttcgctc atggttggat aaaatgaata aatggagccg gctaaaccat
    29221 tgttcgcttt tggtaattaa ttccggaaat aataacgata aacaattttc attgttactt
    29281 gaggaatacc gttcactttt tggtcttgcc agtttgcgtt ttcagggcga ccaacatttg
    29341 ctggatattg ctttctggtg caacgaaaaa ggggtcagcg cccgtcagca gcttagcgtt
    29401 cagcaacaaa atggttgctg gacattagtt caacaccagg aggcggaaat ccaaccacgc
    29461 agcgacgaaa aacgcattct gagtaatgtt gctgtactgg aaggtgcgcc gccgctatcg
    29521 gaacactggc aactgttcaa caataacgaa gtcctgttca atgaagcccg taccgctcag
    29581 gcggcgacgg tggtcttttc tttacagcaa aatgcgcaaa tcgagccact ggcccgcagc
    29641 attcatactc tgcgtcgcca gcgcggtagt gcgatgaaaa tcctcgtacg ggaaaatacc
    29701 gctagcctgc gcgccaccga tgaacgttta ttattggcct gcggtgcaaa tatggttatc
    29761 ccgtggaatg cgccactctc ccgctgtctg acgatgatcg aaagcgtgca agggcagaag
    29821 tttagtcgct atgtgccgga agatatcact accttgctgt caatgaccca gccgctcaaa
    29881 ctgcgtggtt tccagaagtg ggatgtgttc tgtaatgccg tcaacaacat gatgaataac
    29941 cctctattac ctgcccacgg taaaggcgtt ctggttgccc tacgtccggt accgggtatc
    30001 cgcgttgagc aagccctgac gctatgtcgc cctaatcgca ccggcgatat catgaccatt
    30061 ggcggtaatc ggctggtgct gtttctctca ttctgtcgga ttaacgatct ggataccgcg
    30121 ttgaatcata ttttcccatt gcctactggc gacattttct caaaccgtat ggtctggttt
    30181 gaagatgatc aaatcagtgc cgagctggtg cagatgcgcc tgcttgcccc agaacaatgg
    30241 ggcatgccgc tgcctttaac gcaaagttct aaaccggtca tcaatgccga gcacgatggt
    30301 cgccactggc gacgaatacc agaaccaatg cgactgttag atgatgctgt ggagcgctca
    30361 tcatgatgac catcagcgat atcattgaaa ttattgtcgt ttgcgcactg atatttttcc
    30421 cgctgggcta tctggcgcgg cactctttgc gacgcattcg cgacacctta cgtttgttct
    30481 ttgctaaacc tcgttatgtt aaaccggccg ggacgttacg ccgcacggaa aaagccaggg
    30541 caaccaaaaa atgactcaat ttacgcaaaa taccgccatg ccttcttccc tctggcaata
    30601 ctggcgcggc ctttccggct ggaacttcta ttttctggtt aagttcggcc tgttgtgggc
    30661 gggatatctt aacttccatc cgctcctcaa tttggtcttt gccgcgtttc tgctgatgcc
    30721 cattccgcgc tacagcctgc atcgcttgcg ccactggatt gccctgccga tcggctttgc
    30781 tttgttctgg catgacacct ggttgcctgg cccggaaagc ataatgagcc agggttcgca
    30841 ggtggcgggg ttcagtaccg attatttaat cgaccttgtc acacgcttta ttaactggca
    30901 gatgattggg gccatttttg ttttattcgt ggcctggtta ttcctgtcac aatggattcg
    30961 cattaccgtt tttgtggttg ccattctgct atggctgaac gtacttaccc tggcgggacc
    31021 aagtttctcc ttgtggccag ccggtcaacc gacgaccact gtaacaacga cgggtggtaa
    31081 cgcagcggca accgttgcgg cgacgggtgg cgcaccggta gtgggtgata tgcccgcaca
    31141 aactgcaccg ccaacaacgg cgaaccttaa cgcctggcta aataatttct ataacgcgga
    31201 ggcgaaacgt aaatcgacct tcccgtcttc gctgcccgct gatgctcagc catttgaact
    31261 actggtgatt aatatctgtt cgctttcctg gtcggatatt gaagccgccg ggttgatgtc
    31321 gcatccgctg tggtcgcatt tcgatattga gttcaagaac tttaactccg ccacctccta
    31381 cagtggcccg gcggcgatcc gtttactgcg cgccagctgc gggcagactt cgcacactaa
    31441 tctgtatcaa ccggcaaata acgactgcta tctgtttgat aacctgtcga aactgggctt
    31501 tacccagcac ctgatgatgg gacataacgg ccagttcggc ggttttttga aagaagttcg
    31561 cgaaaatggc ggcatgcaga ccgaattgat ggatcaaaca aatctgccgg ttattttgct
    31621 gggctttgat ggttcgccgg tttatgacga taccgccgtg cttaaccgct ggctggacat
    31681 taccgaaaaa gataaaaaca gccgtagtgc cacgttctac aacacgcttc cactgcatga
    31741 cggcaaccat tatccgggcg tcagcaaaac agcggattac aaagcgcggg cgcagaaatt
    31801 ctttgatgaa ctggacgcct tctttactga actggagaaa tcgggtcgta aagtgatggt
    31861 ggtcgtggtg ccggaacacg gcggcgcgct gaagggcgac agaatgcagg tatctggcct
    31921 acgtgatatc cctagcccgt ctatcacaga cgtccccgtt ggggtgaaat tcttcggcat
    31981 gaaggcacca catcaggggg caccgattgt catcgaccaa ccgagcagct tcctggctat
    32041 ctccgatctg gtggttcgcg ttcttgatgg caagattttc tccgaagaca atgttgactg
    32101 gaaaaaactc accagtgggt tgccacaaac agcaccggtc tccgagaact caaatgcagt
    32161 agttattcaa taccaggata aaccgtacgt tcgcctgaac ggcggcgact gggtgcctta
    32221 cccgcaataa gaacgaaaaa ggccgcagag tttcacccct gcggcctgat ccgggcgcaa
    32281 attgccatta cggcagcctg acgcccgcat gacacgttac ttccgctt
//
LOCUS       DAZGAP010000032        31776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGAP010000032 DAZGAP010000000
VERSION     DAZGAP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.032"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..793
                     /locus_tag="ACHLAM_001267"
     CDS             <1..793
                     /locus_tag="ACHLAM_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO7852828.1"
                     /translation="PASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTSVVP
                     RISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQFNG
                     NMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGP
                     ASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLLVRS
                     RAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            797..2128
                     /gene="traO"
                     /locus_tag="ACHLAM_001268"
     CDS             797..2128
                     /gene="traO"
                     /locus_tag="ACHLAM_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO7852829.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            2125..2838
                     /gene="traP"
                     /locus_tag="ACHLAM_001269"
     CDS             2125..2838
                     /gene="traP"
                     /locus_tag="ACHLAM_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO7852830.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCLLIIL
                     AAVWYKFLRAPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            2835..3365
                     /gene="traQ"
                     /locus_tag="ACHLAM_001270"
     CDS             2835..3365
                     /gene="traQ"
                     /locus_tag="ACHLAM_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO7852831.1"
                     /translation="MMRIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            3412..3810
                     /locus_tag="ACHLAM_001271"
     CDS             3412..3810
                     /locus_tag="ACHLAM_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO7852832.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIAQVIGLVLFIAGLLGLKQIGKKPGAGLACIL
                     SIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            3867..4118
                     /locus_tag="ACHLAM_001272"
     CDS             3867..4118
                     /locus_tag="ACHLAM_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO7852833.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            4033..4851
                     /locus_tag="ACHLAM_001273"
     CDS             4033..4851
                     /locus_tag="ACHLAM_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO7852834.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILMPDSFPDKKYVM"
     gene            4906..5265
                     /locus_tag="ACHLAM_001274"
     CDS             4906..5265
                     /locus_tag="ACHLAM_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852835.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            5265..5645
                     /locus_tag="ACHLAM_001275"
     CDS             5265..5645
                     /locus_tag="ACHLAM_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852836.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     RNERRKCIVVHINKITYPPVNSLR"
     gene            5821..8865
                     /locus_tag="ACHLAM_001276"
     CDS             5821..8865
                     /locus_tag="ACHLAM_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7852837.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEVMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            8865..9482
                     /locus_tag="ACHLAM_001277"
     CDS             8865..9482
                     /locus_tag="ACHLAM_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO7852838.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWQDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            9443..10648
                     /gene="traW"
                     /locus_tag="ACHLAM_001278"
     CDS             9443..10648
                     /gene="traW"
                     /locus_tag="ACHLAM_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO7852839.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPIREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            10645..11214
                     /gene="traX"
                     /locus_tag="ACHLAM_001279"
     CDS             10645..11214
                     /gene="traX"
                     /locus_tag="ACHLAM_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO7852840.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            11287..13446
                     /locus_tag="ACHLAM_001280"
     CDS             11287..13446
                     /locus_tag="ACHLAM_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO7852841.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     YYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTTASSTEYRTAEAAEPKSIIMKVWSGVNFADKIATSLSGTGTTSD
                     QLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAKAF
                     LEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTNQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            13528..14175
                     /gene="excA"
                     /locus_tag="ACHLAM_001281"
     CDS             13528..14175
                     /gene="excA"
                     /locus_tag="ACHLAM_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO7852842.1"
                     /translation="MNTTKSRYPSWWAYYQLARNTYFIFGIPCLLFYAFFGTLIVHDS
                     SLSQTRDFIFAYGCWALIILPLLWLYLKTRAKKKKVQQVVQQIKESSPFAPTNDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(14489..14641)
                     /locus_tag="ACHLAM_001282"
     CDS             complement(14489..14641)
                     /locus_tag="ACHLAM_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7852843.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            14974..15216
                     /locus_tag="ACHLAM_001283"
     CDS             14974..15216
                     /locus_tag="ACHLAM_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852844.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(15309..16844)
                     /locus_tag="ACHLAM_001284"
     CDS             complement(15309..16844)
                     /locus_tag="ACHLAM_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7852845.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNALGNVLILFLMMLLGRRNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGHKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(17044..17499)
                     /locus_tag="ACHLAM_001285"
     CDS             complement(17044..17499)
                     /locus_tag="ACHLAM_001285"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFO7852846.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVIYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLAVLCLFFIYSFFKRKRVVGKLLLCIYILYISHYA
                     ITFVYCLYVVGAECMATGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(17587..18210)
                     /locus_tag="ACHLAM_001286"
     CDS             complement(17587..18210)
                     /locus_tag="ACHLAM_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7852847.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRESGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            18359..18844
                     /locus_tag="ACHLAM_001287"
     CDS             18359..18844
                     /locus_tag="ACHLAM_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO7852848.1"
                     /translation="MIKYIFALTLLLSGSVCADQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVVNTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNSHNNY
                     K"
     gene            19488..19852
                     /locus_tag="ACHLAM_001288"
                     /pseudo
     CDS             19488..19852
                     /locus_tag="ACHLAM_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19862..20053
                     /locus_tag="ACHLAM_001289"
     CDS             19862..20053
                     /locus_tag="ACHLAM_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852849.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPGGE"
     gene            20087..20410
                     /locus_tag="ACHLAM_001290"
     CDS             20087..20410
                     /locus_tag="ACHLAM_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO7852850.1"
                     /translation="MAYHSPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMREQMSGIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            complement(20553..20648)
                     /gene="dqlB"
                     /locus_tag="ACHLAM_001291"
     CDS             complement(20553..20648)
                     /gene="dqlB"
                     /locus_tag="ACHLAM_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO7852851.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELID"
     gene            21147..22454
                     /gene="trbA"
                     /locus_tag="ACHLAM_001292"
     CDS             21147..22454
                     /gene="trbA"
                     /locus_tag="ACHLAM_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO7852852.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFAFLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     LGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPSIAPVLADGDSNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTEKQHPADKEKEQ"
     gene            22451..23575
                     /locus_tag="ACHLAM_001293"
     CDS             22451..23575
                     /locus_tag="ACHLAM_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO7852853.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     SPGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            23556..25859
                     /gene="trbC"
                     /locus_tag="ACHLAM_001294"
     CDS             23556..25859
                     /gene="trbC"
                     /locus_tag="ACHLAM_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO7852854.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            26326..26556
                     /locus_tag="ACHLAM_001295"
     CDS             26326..26556
                     /locus_tag="ACHLAM_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7852855.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(26654..27085)
                     /locus_tag="ACHLAM_001296"
     CDS             complement(26654..27085)
                     /locus_tag="ACHLAM_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO7852856.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(27153..29864)
                     /locus_tag="ACHLAM_001297"
     CDS             complement(27153..29864)
                     /locus_tag="ACHLAM_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO7852857.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDMQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYEP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(29876..30208)
                     /gene="mobA"
                     /locus_tag="ACHLAM_001298"
     CDS             complement(29876..30208)
                     /gene="mobA"
                     /locus_tag="ACHLAM_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO7852858.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            30441..30776
                     /locus_tag="ACHLAM_001299"
     CDS             30441..30776
                     /locus_tag="ACHLAM_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO7852859.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKRAPKKTDSGR
                     NDGIDMNNN"
     gene            complement(30861..31709)
                     /locus_tag="ACHLAM_001300"
     CDS             complement(30861..31709)
                     /locus_tag="ACHLAM_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7852860.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT         8087 a         8840 c         7607 g         7242 t
ORIGIN      
        1 acctgcctct ccccttgttc aggaagccgc atcactggat tcccccctga atgcaggtga
       61 aattcgggaa ctgcggggac gaatggccga taacgaacgg gccatcagcg cacctgtcac
      121 cagtgtggtg ccccgtatca gctccctcac ggtaaatctg tcacccgggg ccagtctgcc
      181 gcttgtccga accgcactgc ataacgtcag cgtggtaacg ttcacggaca tcaacggctc
      241 cccgtggcct cagtcggatc cgccttataa cgcggcaccc ggtcactttg atgtgcagtt
      301 caacggcaat atggtctccg tcactcccct gcgcgcctgg gctgcaggga atgtaacggt
      361 ctacctcaaa ggactgtctg taccggtcat tctgaatatc accagtggtg aaaccgacac
      421 caataccctg agtcaggaga tcgacagccg tctggatctg cgcattcccc gtcagggacc
      481 tgccagtcct gtggtagccg taccatcaga gaagattgcc ctgcatgatg ccaccctgca
      541 ggcctttctg gacggtgttc ctcccgatga tcccaccgtg cgtcggctga aattctccgg
      601 aaatgttccg ggcaccacca tctggcagca cggcgatgat ctgctcgtgc gatcgcgggc
      661 catgctgcgg gatgaatttg aacagacgct ggcatctgct gacggtacgc gtctgtggaa
      721 actgccggtg actcccttac tcaccttctc cgtgaatggt cagtccattc acgtcacccc
      781 tgaactggag taaatcatga gcgcagaaca ggacacagcc gcaaagtccg gcaaaaaact
      841 gtcagccctg ctggttctcg ggggcgtgat tatcatcggg ggcggatacc tgctgctcac
      901 ctggctttcc cccgaaaaca gcgcatcgct ctcgtcagtc aatatcaaca gcacgacaac
      961 aggcagcggg cggaccgtgg tggagtcaca gcactaccgg gatttactgc gtgctgacaa
     1021 tgagcgcggg gccgcagaag cgttacgtaa taaccagacg ttcatcgcca gtctgcccag
     1081 gggactggat actgatcacg gaaataatca aagaagggat actcagcctg ctgaccaggc
     1141 cgagggttct ggtcaaccat caacgccccc tcaggcagat caaacagccc gggaaaaaca
     1201 gcgggaagcg ttacaaaaac tgcttgcccg catcaacaca cagcatcctg acgcccgccc
     1261 cccggtagtg gcggctgcaa tgtggggtac cactggtgct cccgggataa gctcacacgc
     1321 ctcaccaccc ggtaccggca catcaccaca accacgtctg caggaggcca gtctggcaac
     1381 cccggcagct cgtacaggga tacagctgat accggcactg acccgcgtac ctgccttcat
     1441 tgatacggaa gtggactctg acaatgctgt ctcccgcgtg gtggcctccg tacctgccgg
     1501 cccatgggcc ggggccgtcc tctacagcca ggacgccaga ctcgccggca agggaatgga
     1561 tatccacttt gaccggatga tgtggcgggg catgtttctg aaagtcaatg catatgccct
     1621 gaatgaacat accggcatgt catccgtggc cagcgacgtg aaccaccgct ggtttaagca
     1681 tatcgttctg ccgtctgtgc tcggtggggt gggcagtgtc ggaagtcttt acaaagacgc
     1741 caacacacag gtcattcagg gaaactacgg cagcattacc ggcagggtgg gaatgcctga
     1801 tggcgaggcg ctggctggcg tcattgcagg cggcatggcc gaacggggca gtcagttgct
     1861 gacccgccag gcggagagcg aaccttaccg gcaggtgaca gtgtcacgcg gagaggttgt
     1921 ctccattctg tttgttgagc cggtcatgag caacgatatc agtcccccct cctccggcac
     1981 tgccccttct gtgccaccgc cctccggacg cccgctcacc caggaagaag cagagcgtca
     2041 ggcgtctgac aggatccagg cagctgtcat gcgtaaacag aacgaaatgc ggcaacgtta
     2101 cggaatacaa caggaaagtc atccatgaaa aacgaacatg aagatcccgg cagttttggc
     2161 gaaacaacga caccggcatc cggcaaatcc ttcctgaaac gccagtatat gggatacagc
     2221 ctgccagtct gggccggctg tctcctcatc atcctggccg cagtctggta caagtttctg
     2281 cgggcaccct cagagccccc tgcagatgca ggcttcgctg tcacaccgcc cgcagccgaa
     2341 agctgggcag agcccatgcc catgacggag accgggagcg gaaacaccat gtccgcgcct
     2401 cctgtcaccc cacaggcaga catgcagcag cttgcccgtg acctgaaagc agagctggac
     2461 aggcgggatg aggaaatccg acgtaccctg accatgctga aagagagcat aacaaccctt
     2521 ggcgacacca tacggaaaga tgaggcctgg gcacaggaaa cccgtcagtt gcttgatgaa
     2581 acccgtaagc aggtgcatgc cctgcagatg cagggaccac tgcaaatccc gaccgggaat
     2641 aacggccagt caacgaaaac cccgccttcc cgcctgcagg gcatgcaaat catgtcggtg
     2701 gaaaaaggaa tggcctggat ccgctggcag ggtagcgcct gggcagtacg ggaaggtgac
     2761 aggctgggga atatcaccat cacccggatt gaaccgtcca gccgctcagt catcacctct
     2821 gccggtacat tacgatgatg agaattgatg ccctgacagc catagagaac tttgccagcg
     2881 gtatattcag tgcaggcatg gagtttctct tcacctgggg agagctgctg gggattatcg
     2941 gcctgattgg tcatctgatg cgtgcccggg ctgaagggcg tcacagtatg ggaccgggaa
     3001 aatttatcgc cggtatcgtc atctgcggca tgctggtcgc cctgccatcc ttcatcaatg
     3061 ccggggggac ccagatggga ttcagggcag attccttcgg ccctatcgct tatgtacaac
     3121 caaccacctt tggtgccgct gccggtgccg ccaatgccat gctctcactg gtaaaactgg
     3181 ccggagtggg gtttgccatg aacggcattt ccatctggcg taaatccctg ctggacgggc
     3241 ataccgccct gagcagcagt gagtccatca gcaaaggaaa cgtcaagttt gttgccggcg
     3301 ttctgctggt ttttatcgat cgcgtcatcg atgcctctct ggcgagtctt ggtctcgcct
     3361 tctgatccac attcattttc agtaattcag tctgttagtt aaggagacta tatgtcacga
     3421 ttccgtgcca tggcggcgcg tctgtgcgct gcatttattt atgttcagtt atgcctggtg
     3481 gcctgccgtg accggctgat acgcctgtta cttctgcttc ccgtgatact gctgccccac
     3541 acagcgatgg caaacaccct attcgatatg atcaaaaccg ttggcgatgg tgcaaaaggc
     3601 tccagcagtt catttgtggc catcgctcag gttattggcc tggtgctctt tattgccggc
     3661 ctgcttggcc tcaaacaaat cggtaaaaaa cccggagcag ggctggcctg catactgtcc
     3721 attgtggtcg gtgcctgtct tgccgtcgta ccggaaatga tgagcagtgg acagaaaacg
     3781 ctcggtcttt ctccggtctc catcagttaa tccacaacgg caccggtttc ccggtgccgt
     3841 cttcacacaa tgaagaggac cagaccatgt cctgtgagct gccccctttc accctgctca
     3901 gaacagagca cggcaacgtg attgcccgcg acaaccggga taatcaggag tttgtgtttg
     3961 cagaatgtta ttccaccgcc atcgcagcac tgattctggc tgccgtcacc ctgtatacag
     4021 agagaaaaac gtatgaacaa aacaccgcca ctcatcgccc cggcccggga acgctggctt
     4081 ttcccgctcc cgcaaccgga cacggtattc agcggtgaat ccctgctgcc accacccgtg
     4141 ctctcaacac ccggacgctg tccctgctgt cgcaggaccg tcactcaccg cttcattctg
     4201 gaggattcat ggccattaca gcagatggct gacacctgcc gggatacggt cgtcctgctg
     4261 gaaaaaaacc tcacccgggt gatgcgcctg aaaaaacatc cggtaccgga aaacgctgac
     4321 gaaaaaaaga aacacacccg aaccctgcag gatgcggagc ggtccctggc acaggcccgc
     4381 ctgtcggcca ggcgactggc cctgcgtcat gtggaaaagt cacaaatcgt cacaacagat
     4441 gcactcagcg aaaatgaaag tgaactgctg cagcctgaag gcccgccctt tcatctctgt
     4501 gccttctgcc acgcctggca ctgcctgaac ggctacgctg ccgcacaggg ggtgatggtc
     4561 tggctccctg atttgcatcc ggcctcggta gttgctctca atgcacgggc actgaaggaa
     4621 attttcagcg atgagcgtaa gcgaatcaga cagggacggg ccgtactgaa tgccctggta
     4681 cagaaccgcc tggccgttga ggagaaattt cgcacctggc gtccggcgga ttttgctgat
     4741 gcactgcgcc gctggccgcc ggcacagcgt aaaacactgc gggagaaaat ggacggggtg
     4801 gcgctcattc tgatgccaga ttcgtttcct gacaaaaagt acgtcatgtg acacacaccg
     4861 ctgccggtga cgttctgccg gccccaactg acagaggaac aaaaaatgga taccctcact
     4921 gccggatatc agttttccgc agatgcattc cggcgcttta ttatccggac gctgactgac
     4981 gatattccgc ctgacggaga tgaaaaagag attgcccggt tcattctccg ccacccggca
     5041 tttaacggcg gctggctgct ctttctggct gcagaaattc atgaaagatc ccgtacccat
     5101 aacagcatca ccgtcacagt gagcgtcgat aactatctct catggatgga tattgccgtg
     5161 tccggtctcc cggaattacc ggatatgccc ttctgtgatt ttttcattcg ccgccagcag
     5221 tttatctatc cgggtgtacc cccgacaaaa tcaggagaag aatgatgaaa attcaccgca
     5281 tatccccgga gacgcttata acactcatcc tcgcacacct ggccggaaaa gctgacagta
     5341 ccgccaaaga agagcaccgg ctactccgtc ggttccttcg ggatgatgac ggcaggctgg
     5401 ccggcatact gctgaatatt gccggaatac ttcagttcaa cagggaatta agcgcccggc
     5461 ataattatcc ggccacaccc ctgactgaat tttcattaag gaaacgggga aaacaactac
     5521 atctctgtct ctgcagtctc cgtttctttt atatcccccc tgtatttata cgaaatgaaa
     5581 gaagaaaatg tattgttgta catattaata aaatcactta tccgccagta aattcactac
     5641 gctgacagcg tgtttccacc ttattacatt tttattatta actgtaatca cacagaaata
     5701 cgcacctgtc cctcaacaga ttatttattt tctctgctgt tttttcatgc cacatcccct
     5761 gctgtggcat caaaaaatat cagaccgtga tgattcatcc ttaccggagt gctccatgcc
     5821 atgaatatca accgcattat ttttgccatt gaagactgta tcagtaccct gtcacggtat
     5881 tccgtttccg cattcttccc cgattactgt gatctgcaca ccgtcatcgg tctggaccaa
     5941 caggaccgcg aacgccgccc ctggctgaaa gccccctaca ttgccaccac caaacagggg
     6001 tattaccttt ccgtctttga aatcagcggt gccctgaaag aaatggatga aaacccggac
     6061 ctgacagccc ccggcacact ggaatcactg attacagcgc tggcagagcg aatgaacact
     6121 gcctataaaa acatcggtca caaaatcagt tttgttttcg aacgcgatcc ggagcgtgga
     6181 aggaatgaaa tcgaagtcat gctcttaccc cagcgaaaat ccatcgcacg cacggggatc
     6241 ctgcttcagg acgttctgga tgaaaaagtc accacgctca ccccctggct tgtccgggaa
     6301 cgctgctggc tgaccgtctg gagctctgaa gaactgctga gccggaccga tcgcaaggac
     6361 caccagaccc gtatcaggaa actcgctgaa cacgctccgc cagcccgatt tgcgcaggat
     6421 ccatggcgct ggacactttc cgcactcaaa atacggcatg acgcctttct ggacacgctg
     6481 gagcaggccc tgacacatga ttctgatggt ctgctggttc gcctgatgga tattcatgaa
     6541 gtgggacggg aaatccgccg tcaactggag cgcaacagca cacctgcagt atggcagcct
     6601 catctgcctg aagatgcccg tcctgccggc tggcgacagg gcgaggatac ctctgtattc
     6661 cacgcaccgt cgctgaatct gcagttattc accacacagc ctgaaacaca cggcagcctg
     6721 ataaaggccg gtgacctctg gcacggtatg gtcgccatta ccctgccccc ccagaatctg
     6781 cggacattca accagctggt cagggatgtg ccccgcgcaa tcccctggcg tatccggatg
     6841 gatctgatgc ccggcggcat gaaggcgctt ggggtcaaga aaaccctgct gacctacagc
     6901 agctttattt cccctctccg gcccatgtat gactcagtga tgatgctgaa tgacatcaac
     6961 aaacatgatc cggtctgtat catgactatt gttgccacca catggggaaa cagccgggaa
     7021 gtctgtaccc gcaaccaggc gctcctgaaa tctgcgctgg aaggctgggg agtatgcggc
     7081 accaccacca ccttcggtga tccgagacgt gcctgggtga acacggttct ggcggcctcc
     7141 cacggttccg gtccagtccc cctttacccg cccctttcac acgccctttc cctgtttccc
     7201 ctgaacaggg ccggctctgt ctggcgggga cagggaaatc tgatgatgca caccgaagac
     7261 ggctctgcat gggaggtcgc gctggcatcg tcacaacaga acaagcacac ggaactgacg
     7321 cccggcgcac ccggtctggg caaatcggta ctcatcaacg cactgtcgga gatccagata
     7381 gcctcagccc agaagaacct gcctttcatt gcctatatcg acaagggatt cagtgcacag
     7441 ggactggtac agcttatccg tgacagcctg ccggagcaga gaaaggacga agccgtgggg
     7501 atcattctga gcaatgatcc ggaacacacc cgtaacctgt ttgatgtgat gtacggtgct
     7561 aaaaaacccg ccaccccgga aaagaacttc atggtatctg tactctgcgc actgtgcgtg
     7621 gataccggca ccggtcagcc ctgtaatccc ggtgacaccc ggcaaatcat aagcaacctg
     7681 gtggatctgg ccttcaggga gtatggcgaa aacaacccca gactgtaccg tgcaggaaca
     7741 gagccccttg tggatctcgc cctggaagag agtggcatcg cagaacaaca tgacgctgga
     7801 tggtggaatg ctgccacctg gtttgaaatc agggacatgc tgcatatggc tggcaatatc
     7861 cccgcagccc agcgggcaca ctaccaggcc atgcccctgc tggctgaaat gtccgccctg
     7921 ctgggacaac catccatccg ggatgtgttc ggcacagttc agcgcgataa ctcagcagaa
     7981 ttgttactgg actacatccg ccgtgccctc gatcagggac acagtgacta cccgatgatg
     8041 tccggctgta cccggtttat gctcagcccc gacacccgcg ttgtggccgt tgatctgaac
     8101 aatgtggccg gtgacaagac acctgccggg cgtctgcgca ctggcatcat gtacctgctg
     8161 gcaggacaaa tcgccggcgg ggattttgtc ctgccgcagt atcaggagga aatacggaaa
     8221 aatctcgacc cacgctacca tgaggtgatt ttccgccgca ttgaacagct tgatcaggag
     8281 gtcaagacca aagtctatga cgaactgcac aatgcaaaag ggatcaattt catctgggaa
     8341 gccctggaca cccaggagct ggagcagcgt aagttcggga tacgcacagt gctgagcacc
     8401 cagctattgc aggactatcc accggcagtt ctcaaatccg ccaacaccct ctggttgctg
     8461 cgttaccgcc ctgacgaaat tccgtttctg cgggataatt ttggtgtccc tgaagtcacc
     8521 ctgcggcgct tcctgaaaat gccggaaggg gctgcaccgg atggcagcgg tgtcccggta
     8581 ctggctgtgt tccgtgtaaa aaacggtacg ctggcccgga tcctgaaatt cactctcggt
     8641 ccactggagc tgtgggcact gaattcctca cccaaagaca gcgccctgag acgtgcgctg
     8701 acgcaggagc tcgggtcact gcgggcacgc cagatcctgg cagaaaactt tccccgtggt
     8761 tcagccacct cactgataga acaccgcgcc cgtacacatg acagtgaaaa cgtcatccat
     8821 gatctggctg cagaactcat ccgtaagcag ggatatcacc tctgatgtac agcatacaca
     8881 tcactccccg ctgccggcgt cctgcacggg gacaggtctt cctgaaaacg ctgcgcgtgg
     8941 acggtgacag tttcagcttc accctgccgt ccgcctgctt cacgctgcgt ccctccccca
     9001 atccccgggt cattttcgcc agtgacagac gggctgacgg ggaaatgagt ggcgttcgcc
     9061 tggctctgcc cggagagaca ggcacgctga ttacaacggc cacctggcag gtcaacgtgc
     9121 cggagcggcc tccttttgat atacagcatg tcattcactg gctcctgccc tcgcctctca
     9181 cggcgctgat gtccgacaac acctggctgt ggcaggatag ccccggcaca ccggcactga
     9241 cggcgggaga ctggccggta atggaaaccg ataccccgac gctgaaacgc gtgaagcgtc
     9301 gtaccttcac gctcatcgat aaccgcaacc gtcagggatg gatcacccgt cgcttccagt
     9361 gcttcccgct ggctccgctg ccggaatcac acagtcaccg cgtaccagac atcctgtatc
     9421 ccctgaaaac ggagaaaaaa gaatgcaaag acaaccagca accacactgc ttgcactcct
     9481 gataggctgt gcggttatcc cgtccaccca cgcctattct gtgaccgtag tggccagcca
     9541 gcctgtcacc acacaaatca tgccggagct ggccgccatt cagggcgtga tggcagagat
     9601 ggtccgtatg cagacagcca ccggcacagc catcaaccag agttcagaga aactggcctc
     9661 tgtcattgcg caggatgggc aggccacccg tcagcagatg atattcagta atgaaacccg
     9721 ccggcttgag gaagcccgga agagcttcag cgtgccggac tccatctgca gtgaatccgc
     9781 ctccggagtt gcagctgaaa gtcgccgtgc agcagcgtct gcggctgcac gtctgagtca
     9841 gggaggaggc gtcagcagta aaccaatccg tgaacgtctc tcccgggcag cagactcacc
     9901 aatccgcgag gcatatgaca gtgcaggcat tcatgccggt tactgcacgg aagctgaata
     9961 tgcccgcttt ggcggaaccg atgtctgccc tgccgtgggc gagctgccgg gcggagacag
    10021 ccaggtgcga tcgctctatc agggtgcagg cacagcagat acccctgccg ccctcacctg
    10081 ggatcagaaa cagattgatg ctgccacggc ttacatgaaa aacaccgcca gaccgtcagc
    10141 gggtcgtgcc ccggggaaag gtgaagtggg aacacagacc gggcgcacgt atgtgggcct
    10201 gcagaatgaa tacaacggca tcatcgatgc cgcttctcat ccgcaactgt cactgatagc
    10261 tgacagtacc cctaatgagg ccacccgtgg agcgctgact gaagccctgc agtcaccatc
    10321 cgctgcggca tattttgacc ggaccgcatc atcagaggcc cgcacccgtg gtcacatgag
    10381 ccagcgggag tttgaggcct ttgaagctgg cagacgctat gcaaacaccg actggcagca
    10441 ggatctgcag ggcatggaag gcgataacct tctccgtgaa ctgctgcgca ctaccgccct
    10501 gctgaactgg cagatgaacg acctgaaaga gcagatacgc cagggtaatg ttatcgccgg
    10561 gcagcagctg gcactggcgg cacgacagta ttacggacag cgtctcggtg aactgtcaca
    10621 ggccatgagc caggggagcg taagatgaaa gacgacaaca gcaccgacaa aaaaaaaccg
    10681 gccccccgtc tccgccgcat cggtgacatc gggataaacc tgttcgcacc actggcagaa
    10741 acccggtgga tgctgcgcag ctgccgccat atgctgggcc agcataaaac gcgcctgaag
    10801 cgcctcatgc ccgaccggca accggagagt ccggtctgta tgacctggga agaagccgtc
    10861 agggccagcg gccttacggt caacgaactg gatcggcgtc tgcgacgtcg caggatgttc
    10921 tggcggacag gattctggct gatgatgata ccggtacttc tgctgtgcgg catggccctt
    10981 gctgccacag cgcttccggc catcaccctg atacgtatac tggcctgcct gttactgatg
    11041 agtgccattg cagcactgtg tgcctcaaaa accctgattg tgtcataccg cctctggcaa
    11101 ctgaatgaac ggcgggtcag cgaggacgaa cagggcacct tccccgatta cctgcaggcc
    11161 cggcacggct ggaaacaggc cacatggctg gccattaccc accggcagta ctgaccgtcc
    11221 ggtctgcaat tccttttcct cattccggag gctgatgccc tgcatccctt cggttccgga
    11281 gttatcgtga aaatcatacc cagagcactg agtgcgggtc tggcgctggc tgccgtacct
    11341 gcacaggcat tcgtcaccta tcaggacatt gtgagcgccg ccaccaatcc ggatgacctg
    11401 tcgcgccagg cactggtcac catttttggc gacgtggtca caaatcccct gacagccagc
    11461 agcaacacca ttatcggtaa cctgttcatg gtgtttaacg caattatcgc cattatgggg
    11521 atagtctggt tcctgtttat tggaatccgc catgttgtcc gcaccggcca ccagggcaat
    11581 gtctttaaca acggacggga cgtcgtcgga atgctcagta ttgtgagcgg ttttatgatg
    11641 attgtgccca ccgcttccgg ctggagcctg gcacaactgg tcatgctctg gggcgcatcc
    11701 atcatgggca ttggttccgc caatatcatg gttcagactg ctgcagaaaa catcgctgaa
    11761 ggctattcaa tgaccgtaca gccagttcat gtctccaccc gcacggcagc acggggaata
    11821 ctggagatgg cgctgtgcaa acacgccatg aatgcaggcc tcgatgactt taaccgtact
    11881 gcccgttcat caacgccccc catgagcgaa tcctcacgga cagagaacgg caggtatacc
    11941 gtcaccgttt caaatggcag tgccacctgc gggagtgtga gtctcaccgt gaaagggaat
    12001 ggcagcaaca accagtccgc actggactat tttttcaatc ccttcattga aacggagtat
    12061 cagggtgtcc tgacggcaca acgctccgcc atggatacca tgctccgtga cctggacagc
    12121 gcagcacagg aatttgtgtc tgcgtaccgg acccgacgcg acacgggtaa cggcaacctg
    12181 ccggatattg aaagccgtat tcagaaggct gccaaccggt atgaagaaac ggtgcagaag
    12241 gcgttgccaa cagataacgc agaaactgag cgcagggagc agctgaaata ctacctgacc
    12301 acctacggct gggttgccct gggggcctgg taccagacct ttgccaccgc caaccagcga
    12361 ctggcagaac tggccgaccg tgcccccact gtgagtggag tttcatcgca gggcgaagcc
    12421 ggagatactg aattcctgaa ggaagtaaaa ggagcttacc aggcacagtt gcaaaactca
    12481 ccatatacac ctgcactggg aacaaccgct tcatcaactg aatacagaac agcagaagct
    12541 gctgaaccta aaagcataat catgaaagta tggtcgggtg taaattttgc agacaaaatt
    12601 gcaacatcct tatctggaac aggaaccacc tcagaccaac taaatcctct gataaaaatg
    12661 aaaaatatcg gtgattacac tatggtcaca acagaaacca tctgggcggg ttataccacc
    12721 gtcagggtac tttccactgc cggcagcaac tctatttttg gtcgactggt caatgccgtt
    12781 agcggcttat ctgatgccgc aaaggctttt ctggaagcag ctgcaccacc gctctacttc
    12841 ctgctgtttt tgctgttctg tgccggtttt tcgttatcca tttacctgcc gtttattccg
    12901 tttatcttct ggatgaccgg catcggtaac tggatagtca gtgtactgat tggatgcgcc
    12961 gccggtcccc tgtgggctgc cacccatctg gggacaaacc aggaccgggg aagccgggct
    13021 gcttacggtt acatctacct gatagacagc atgatcagac cactgttgat ggtgttcggg
    13081 ttcttttttg catcggtcgc catcgtggct ggcggcacca tactgaatgc gttgtttggc
    13141 gcggctctgg ttaatgtgca ggctgactcc cttacagggt tattcagtat cgcgggattt
    13201 ttgatgattt atgcccgtgc ctgtaccacg ctggttgcgg ccgtttttgc gcttcaggct
    13261 tatctgcctg accatgtaat taattttctg ggcggacgtg acggcgttaa tatgctgggg
    13321 aatatggttt catccgtcaa agatattatt gccggtggca acaccaatat acgcagaaca
    13381 cctggcgttc gtgaagaaaa actgaaaaac attaatggag atgataaaga cggaataaaa
    13441 caataaagta cagacatcaa aaaaaacagt attgccacat tcacctgaag agattaaaat
    13501 acattcaggt gaatatggag gtttattatg aacacaacaa aaagcagata tccttcctgg
    13561 tgggcatatt atcaactagc ccgcaatact tactttatat ttggaattcc atgcttatta
    13621 ttctatgctt ttttcggaac actaattgtt catgactcaa gcttatctca aacaagagat
    13681 tttatttttg catatggatg ctgggcatta ataattctcc cattactctg gctatatctg
    13741 aaaaccagag cgaagaaaaa gaaagtccag caggttgttc agcagataaa agaaagcagt
    13801 ccctttgcac cgaccaacga ttacgagcaa ctgtcattct cgaagagttg ttatttcggg
    13861 atcgacatta aaaacggcac catgctgtat gtgcgcattt acccaaacaa tgtcatggat
    13921 gtgattggcc tggatattca caacttcacc cgaacagttg ctgaagacgg caagctggaa
    13981 atccacacca cctatgtgag tctgccgatg atccctcttg aaatctccgg catctcagca
    14041 aggacgctgg ccaacaccat acacaccatg gctgcacgtg gatatgagta caaagagcgt
    14101 ttcccgcaga tgatccggta tcgggttaaa gagtgggaaa aagttgccgg tgttcctgtt
    14161 gcagaagtat tttgaggtag ctatcaactg ttagacgata atccgtcagc cggttttctc
    14221 tgaaccggct ttttcttttc aggcagaatg cctgtctgcc gtccctgtat cagactgtaa
    14281 ttagcacaat acggcaattt gccgttcagt atattcagga agtacaaatc atgccatctg
    14341 caacacaaac tgcccgactg gaagcaagga tcagcacaga actgcatgct atgctgaaac
    14401 gcgctgcaga aatccaggta aaaaagggcg cattgaggtt tgctttcgca acaatccggc
    14461 ggcagatttc ctgccgccgt gttgcccatt aacgtttaac ttcgtaggct aacgttgcca
    14521 caagcactgt gtttccctgc tcgacacgga atccgcaaag cgaatccctc accatccaga
    14581 caaaacacag tatcgtcaca cagacgacaa ttaacattgt taaaaacgtt cgttgtggca
    14641 tcgcagcccc tccattgcct ttcggccgtc atcgggctac tataatgttg tctaggcatt
    14701 acagtgggcc ctgggttgat tgataaaaaa atcgcctggg gctttctgct gcatgccgtc
    14761 cacaaaacgc tttcagcata cagcctcaag cgcccgccgc tattctaatc gattttcagt
    14821 ctgccacaat cgttttccac ctaatgacat gttatgcaca acagtatttt tacgggaaac
    14881 tacacggcaa ggattcccgg aacacgttca ttatttgatg gttacagaca tcatgttaaa
    14941 tccggaagta ataatgacct gaaaggaaat actatgagta acgaatatca aagcaccagt
    15001 ataaaccctg atgtttttga acaatttctt accctcctga atacacactg taaatcatca
    15061 gaagctgcac ttgccatgct gagtacactc tgctacgaac tgatgaggag gagtgggatc
    15121 cattcactgg acagcctgat gctcaacgga gaaggtatat tgatacaatt acagggggct
    15181 gatattcgcg aaaaaacagg tcagcatatt cactgatcca gggatgtgct gaaaacagtg
    15241 tcagcctgaa ataaacgttc aatgcctcat acacatgagg cattgagcac ggtcatcaat
    15301 aatctttatt atttttctga ttcagcattt ccggtcagta tcggctcttc tggcaaagac
    15361 attaccggaa cgtcacgttc accattgaat acagaaacac tttcagcgaa atcattctgt
    15421 aacaaagaaa aacttaacgg cagatttttg gtttgtatcc ctgtgatcca ttgaaaaatc
    15481 tcgggaaaac gacgtgcact gatatgcaca tgattgaggt ggtgctcagt aatatcactg
    15541 gccatcatca tgagaggtac atcgtatccc gaacgcaaac ctggcgcatg atgcacaggg
    15601 gtgctgtctg aactgacgga ttgcccatga tcagaaaaat acagcatggc aaaatcatca
    15661 ccatgctctg tcgtcatgtt ataaattgcc tgaagaaacc gatccagttt aagcagtgtt
    15721 gataaataac aggatacttt atgtccgaat aattccctga atggctgtgc atcattaaaa
    15781 agtcggtcac agggattagt gtgagacccc agcatatgaa taaaaatcac ttttggagaa
    15841 gacgagttaa cagcttccct gacatactga agcatatcct catcatcaat gcgaagggaa
    15901 gaagagctcc cttccttaag aaaatgtttt ttatccgcat tggcggcgat gacagaaatc
    15961 agcgtatcat gttgccctaa agagccctga tttgatatcc accacgtctc atacccggct
    16021 tttctggcaa gcgtaacaat attattaccc ggattacctg tttcagtgta atcaagcgtc
    16081 agtgttctgc tgagtgaacc tatcgtattc ggcgctgctg agaaataccc attaataaat
    16141 atacccggag cagaattcag ccatggtgtt gtcggcaaag gatacccata aacagaaaga
    16201 taatcccgtc tgacactttc accaattacg ataattatgg ttttatattt cttctccaca
    16261 ctcacaatat cccatgagtc tttctgattg tttttcagcg attcatatcg catcatttct
    16321 tctctggttt cagctatccc tgtcattaca ttcgtaacca tacgttttgg ccatgcattt
    16381 actgacaata cgataaagaa caacagaaag ataaatcccc ggtggctttt atcacgctta
    16441 cgccagaacc agaatgtaaa agcagcaatg accagcgaaa caataaaata aagaacaggt
    16501 atgcctgtga taaactcacc tgcctcattt atatttgtct gcattacaga cgcaataaaa
    16561 ctgttgttta ttttcccgta aatcatccca accggagcat agaatgcact taacaaaaga
    16621 aagatacaga cacacgttgc tgaaataaca ttccgccgcc ccaacaacat catcaggaac
    16681 aatataagca cattccccag ggcgtttttc actgtatatc cgcagcacca tgtaacaagt
    16741 acagaaaaca acagataaat cagtgactct ttacaccaca acataaaata aaactgtttt
    16801 gctctctcaa aagcatattt cgacttttct ttaatacaag acacgccagt cattttcctg
    16861 tacaaacaca gacgatcatt tcaacgcatg gatatttata tgaaacagtt cacaaaaata
    16921 ccctaccctg tccagcaggg tttacatatt atcaggttct gtgtttacag caactgctcc
    16981 ccgaaccaga ttattcgcat aacccgatga aggcctcata accatcaaac actcagaata
    17041 catttagtta acaaaaatat gtcttgccct cccggaaagg caaacatacg aaagagaaag
    17101 catggctata ataatatcaa tcaaaatatg ctcaatcttt ccggttgcca tacactcagc
    17161 accgacaaca taaagacaat aaacaaaagt gatagcatag tgtgatatat aaagaatata
    17221 aatgcacaga agtaatttcc cgacaactct tttccgttta aagaaagaat aaatgaagaa
    17281 caaacataaa accgccagaa aaagaaatgt agctatagat attataaaat actccgtcac
    17341 ctgtgaattc tcatccagag atgaatacag cgaataatat gtggttgtga gattaagcct
    17401 tgttatcaat atataaataa ctgataatag tataatataa agaatcagac caaatctgtt
    17461 tttctgcgct ccggttaata cttctgattt atctcgcata aaatatcctg tctgtaatct
    17521 gtcccggtaa acaatggtaa atttcagaat cagtgcgaat acggtaacaa agattcctcc
    17581 tgtgctttaa agtgtgtagg cagaaaccag tctggtggtt ccggtcaggg agtctgatac
    17641 ctcaacttcc cgggtgacta taaccatacc tgactgacgc agtctctgca catcagctgc
    17701 aattcgctga gttcccagcc agaacaggcc atccagtgcc gttaccagca acccgctttc
    17761 cagcgccagt ttcagccgtg ccgtcggggc tttaacctgc ccggcgatat cagtaaaaca
    17821 acgcgtcgtc atgccatagc agtccatccg ccgtattcgc gtcgcaagac tgtagctgaa
    17881 acaatccggc acgccggata agtcattctg cagactgtat acacatcccc gctgccgccc
    17941 gttgaacagg gcttttttct gactgagcag ccattcccga cgcagagtgt caatcatctg
    18001 cggtgtccgg gttaacagca gacgaaacgg cagctcaaaa ctggtcacat aatcaacatt
    18061 cagtaatgct gtccgcagtc gctccctggc acctgccccg ctctcccggc attcacgcat
    18121 gacatcagcc cactgacgca tcagtcgtgg agactcattc ctggccctga actgatattt
    18181 ctcaatatga taatcaatac gttcctgcat atcgccaccg gatatctgtt gtttacccag
    18241 acacagtgat tagcctacag gaataaatat cctccgtgaa gccaggtgcc tcagaagata
    18301 aaaacatgta cttttttcaa cgtgtaatat taacaccacc tccacaggaa aatataaaat
    18361 gataaaatat atattcgcac tcacacttct gctttccggg tctgtctgtg ccgatcaaat
    18421 tcagggactc gttattcgtg tgctggacgg agatactctg gagatattac aggcccggca
    18481 tcctgtgagg attcgtcttg ttaatattga tgcaccggaa aagaaacagc cttttgggcg
    18541 ctggtcaatg aatcaactta aaagccttgt ggtaaatacg cccgtaaccg tgacatacac
    18601 acagacagac cgctacgggc gtattctggg acgggtatat accgaagacg gcatggaagt
    18661 taaccgctgg ctggtacagc atggtgcagc ctgggtatat cctgatttca acacagatta
    18721 caccctgccg gcataccaga gagaagccag aacaatgaaa cggggtctgt gggcagacaa
    18781 aaaacctgtt ccaccatggc tgtggcgaaa agaaaataaa aatagccaca ataattataa
    18841 ataagacatc tcacagaaat atatttcctg ccgttttatt tcccctgcca gtccccaacg
    18901 ggaaataacc cacagaaatt acacagatgt gaataataaa cctgttcgat gtatccgtca
    18961 caaaagagtt gatgtttgac tcagaagaaa atacgcctga agttaaggaa ggcttcagct
    19021 tcatgattgt tctgacatga acatatatcc ctctgtagcg ccctgccttt cataacattt
    19081 ctattacagc aacaacgaaa gacaacagca atgtcggcct tttacgtcca catgaatatt
    19141 ccacttatcc ggaattgtga cacagcccct gaatacagga ttgattgcca gtgcccacaa
    19201 atggcacatt ttcagtaaga ttctgaatgt tacattcccc cgtcatgtgt ggcacaactg
    19261 tcaccgggaa agaaacggaa agcacatgaa aaacatcaaa actcacaccg gactccttat
    19321 ccacaaagaa caaacgcggc gcgttcgtct tcatgaaact ccggccgcct ggtgccacac
    19381 acgccgggaa tgctacagca aaacgaccgg tcgtcgctgc ggcagccctg acagtctcag
    19441 caggctgatt ctgagcagca ttcagcccat agaatgagat gacccgtatg aatgatggca
    19501 gacagacgcc tccgtcccct gaggcatgga aaatccggtt tctggcgaac ggtacacccg
    19561 gcacatggtt ttacacggat gagcagccgt cacaaaagag tggctgcggg cacattgtga
    19621 cgatgtggaa gtgatccccc tgtgccgtat gcatgacggt cccgttttcc gggaacacca
    19681 tattaatgag ctcagcgccg cactggccag aatgctccat atgcaccaca tactgctgga
    19741 acgaacccgg tacagagaaa atatccatga tgcagaatgt attcgtgaaa ttcacgaagc
    19801 tcccctgcag gctatcggca tcctggaaaa atacaaacgc acaaaatcat aaggcagttc
    19861 tatgtggata ctgattttct ggctctcatc cccctttaat gtctcctccg gcgatgcccg
    19921 gcaacccaca ctggccgttc acagtcagga gttccgtact gaaaaagcct gtcgggatgc
    19981 cttcaccgcg ctgaaaaaag aaaacgtggg agaattcgct ctccggggcg tctgcgtccc
    20041 cggcggagaa taaaccggag atgatgatta gccacaggag tattcaatgg cataccattc
    20101 cccggatatc acactgcatt atccgtatct gcgccggatg tcctgggcgc agataacggc
    20161 agcagctgaa gatgcggaaa tacatcatga ttatgcccgg gcattaatat tgtggcagca
    20221 tgcatatcat gctgccacgc tgaccctcaa caaaaacctg gcagttgcta aaatcgactt
    20281 ctgtattaaa cgtatccgga tgcgtgaaca aatgagcggg ataatccgga agactggcac
    20341 agacgaaata ctcttccgtc tgagtaagca ccatcattta tatgagaaaa agaaaacaaa
    20401 agagggatga caatcccatg atatgggaaa aacttgccat gcttactgca ggtgcatttt
    20461 tgatactggc agtcctgtgg gggtaataat atcctgttta ttaaagggca gaaggcggtg
    20521 cccctctgcc cttaagtacg tttccgggat tatcagtcaa taagttcccg gatcagttcc
    20581 aggattgcta tcactgcgtt agccacagca acaaccgccc tgagaacgat gattaacagg
    20641 ttaatcatcc gtttctccgt caatggagga aatcactgtt gtttactctt acattgcctg
    20701 tcacaacagc tctttcattg ttagatgtaa agtgatttct ctccagccag agcactgaac
    20761 acagcaccac ctgttcgatc agccaggact taaaatctcg cgttcatcgt cccattggag
    20821 atcacggcag aagcatcacc agacagagct aacggagcga ttatagtcat ttttctgatt
    20881 gcaaaaaaga gaagcgcagc ctactatgat ggtgttagat gtagagtgat ttccctttcg
    20941 gtcagagtca ccaactccgc cccggaatag caaaaaaacc gacctcacca gtcggttttt
    21001 ttgtgcctgt ctctcactgc cttctgctgt tcctgtgaaa ataccctggt tatttcatcc
    21061 ctgcccggtc attaccggcg attgttcgca ttaattccac tcagcctcat cccgaaattt
    21121 atccatttat cgatggagga atactcgtgt cttaccaaca ctcacccaca actgaagacc
    21181 cgatgaatat atggtatatc gttggcacca tatgcctgct ctttgcgttt ctcatctggc
    21241 tctttttacc cgacatcgtc tttgccacct gcctgatcct gcacacaatg tgggggatga
    21301 ctgactgggg gccttttcac cattttgccg ccccccgtta taacctgctg gccaacaccg
    21361 ccaacaacgc tgcaacgatc actttcagcc agtggctgga tgtcatgtca cagaccgtgg
    21421 ggatcctctg gctgattctg cttcccatga cgctcggctt tctgtggatg tggtttcagc
    21481 atccggccca gccccgcttc acccgcagac cactgaatat ccacacgctg ccacacctgt
    21541 tttcatcact gtcaccgtcg atcgcccccg ttctggctga cggagacagc aacagactct
    21601 tccacggaca aaaacgtccc gagcgccgtg tcgcactaac ccccgaagtc tttgtggagc
    21661 agaacaacct tatccgcaac atgcagctcg atgttgcgtc cacacgccag tgcttcatgg
    21721 cgcaactggg gcaaccactg accagctgga aagacatggc cccacatgaa aaagcgctct
    21781 ttgccatctt tggcctgcag ttcttcctcg gtgaccggaa agcagcggta gccctgatga
    21841 acaacctcaa tctctcctgc aggctcaaaa gtaaacggga ccaggggcgt ttcagcacgc
    21901 cggtgtattc actggccaga aacgccttta tccgggtcat caaaacagaa ggcgcacaac
    21961 agtggctcag gcagcaccgt tacgttcgca gcggtctggt ctggctttac gcacatgatt
    22021 tgcgcctgac gcctccgaac tggctgtggc tcaagggcgt tgaccggacg ctcttctatg
    22081 cccttcaccg ggcaaacacc acaaaaggct tcatcgaagg ggccggcgtg gtggccgttg
    22141 cccgggcaga aaatgaagcc agccgtctcg gcctcccctg cccggaaccc tgtgtggaag
    22201 aggccattga agggcttcga cgggacatgc tggggctggg cctgatatgg gacgaacctc
    22261 agccagatcg cgatcgtaaa cgtcagatca ggacccgctg gtcactcacg gatgacgtga
    22321 taccccgccg ccatgataac gacgaagaca ctgacactgg tgaaaccacc gaaaccaccg
    22381 aaaccaccga aaccaccgaa accaccgaaa ccaccgaaaa acagcatccc gccgacaagg
    22441 aaaaagaaca atgaccatcc actacactgc aacactgaat aataaacacc tgtccctgac
    22501 cgccctgtac cctgacggta atcgggaggt gattgccatc ctgccagtca cagaactggg
    22561 cgcactgatt aaccgtaata acaggctgat gcacctgtcc ttcagttccc cttttcgcga
    22621 aataaccgaa acggtgcttg ttgataccgt tgatgcagaa cgtcccggac atcacatcgg
    22681 cagaatgctc tcagcgattg atgcctgcct ggatgccgaa tatgccggaa aaggaaaaca
    22741 tccctcccgg aaaaatggct tctggcggac actgatgtgt ggctttatcg ccggaattgc
    22801 tctgccggcg acagttgcag gttaccactg gatgaaggca gagccccctt ccacgctggc
    22861 ttaccctaca tctcaggaat ggagagctga accagtaacc gacacagaga caccacgccc
    22921 acatacggaa catcatgtta acagcatccc ggcccgcccg gctcccacac agcaggaacc
    22981 ttcttctgcc cccgccccga cggcagtgac ggaagaacaa cacgttcagg ccgctgaggc
    23041 ccgccgtaat ctggccacag tacttaaacg caacgcagac cgggggatgt tcaccattaa
    23101 cctgtcccct ggtcatgaac gcacgctgta cgccttcctt gaccccgcat gcatcaactg
    23161 ccgtctgctg gaacccgcgc ttaagcaact ggcccgggaa ttcagcgtgg tgatttatcc
    23221 ggtctccgtg attggcgggg agtccagtac aaagcggact gctccgctgc tgtgtgaacc
    23281 ggactcacaa aaacgggcga ccggatggca tcagctgtat tccgctgaca gtggtatggc
    23341 ccccaccggc gatgcaccgg ccgcacagga tggacagtgt ctgaccgccg cacgtgctgc
    23401 catcgatgtc aataaccagg ctttccgcca gttcggtttt ggcggaaccc cctgggtgtt
    23461 atccgatacc ggctggcacc tgccgtccgg catcctgaaa gacgccactt ccgttcgcct
    23521 cttcctgaaa gcaacagata gcgaggagta tcacaatgag ccagcaccac gttaatcctg
    23581 acctcataca ccgcacggcc tggggaaacc cgttgtggaa tgcactgcat aacctgaaca
    23641 tcactggtct gtgtctggcc ggcagtatca ttacggcgct gatatggcca ctggcccttc
    23701 ctgtctgcct gctgttcacc ctggtcacct ccgtgatatt catgctgcaa cgctggcgat
    23761 gccccctgcg tatgcccatg acactcagcc ttgatgaccc ctcccaggac agaaaggtca
    23821 ggagaagcct cttcagcttc tggcccaccc tgtttcagta tgaagcggat gaaacattcc
    23881 ccgcccgggg gattttttat gtcggttacc gccgtatcaa cgatatcggt cgtgagctct
    23941 ggctgagcat ggatgacctc acccggcatg tcatgttttt tgccaccacc ggtggcggta
    24001 aaacagaaac cacctttgcc tggctgctca acccgctctg ctggggaaga ggctttacct
    24061 ttgttgacgg caaggcacag aacgatacca cgaggacaat ctggtatctc tcccgacgtt
    24121 ttggcaggga ggatgatatc gaagtcatca acttcatgaa cggcggaaaa tcccgcagtg
    24181 aagtcattca gagtggcgat aaatcccgcc cacagtcaaa cacctggaac ccgttcgcat
    24241 tcagcaccga agccttcacc gccgaaacca tgcagtccat gctgccccag aacgttcagg
    24301 gcggtgaatg gcagtcacgg gcgatagcca tgaacaaggc actggtcttt ggcacaaaat
    24361 tctggtgcgt ccgggaaggt aaaaccatgt ccctgcagat gttacgggaa catatgacgc
    24421 tggaagggat ggcaaaactc tattgccgtg gccttgatga ccagtggccg gaagaagcca
    24481 tcgcaccact gcgaaactac ctgcaggatg tcccgggatt cgatatgtca ctggtgagaa
    24541 cgccgtcagc ctggactgag gagccccgta aacagcatgc ttatctctcc ggtcagttct
    24601 cggaaacgtt cacaaccttt gccgaaacct tcggggacgt ctttgcggca gatgccggcg
    24661 acatcgatat tcgcgacagt attcacagcg accgcattct gattgtgatg atccctgcac
    24721 tggacacatc ggctcatacc acgtcggcac tcggtcgtat gttcgtcacc cagcagagca
    24781 tgctcctcgc ccgtgacctg ggctaccggc tggaaggcac agatgcacag accctggaag
    24841 tcaaaaaata caaaggcagc ttcccctata tctgtatcct tgacgaagtc ggtgcctact
    24901 acacagaccg cattgctgtg gaggccaccc aggtacgttc actggagttt tccctgataa
    24961 tgacggcgca ggatcaggag cgaattgaag ggcagacctc cgccaccaac accgccacac
    25021 tgatgcagaa cgccggcacc aaattcgccg gacgcattgt cagtgacgac aaaacggcaa
    25081 gaacggtgaa aaatgccgcc ggtgaagagg cacgggccag aatgggcagc ctgcagcgcc
    25141 acgatggtgt gatgggtgag tcctgggtgg acggtaacca gatcaccatc cagatggaaa
    25201 gcaaaatcga cgtgcaggat ttgatcagac tgaatgctgg tgagttcttt accgttttcc
    25261 agggggatgt ggtgccgtca gcctctgtgt atattccgga cagcgagaaa agttgcgaca
    25321 gtgatccggt ggtcatcaac cgctatattt ccgttgaagc cccccggctg gagcgtctcc
    25381 gccgtctcgt ccccaggaca gtgcagcgca ggctccccac accggagcat gtcagcagca
    25441 tcatcggtgt actgacagca aaaccatccc gtaaacgtcg taaaaaccgt acagagcctt
    25501 accgtatcat tgatacattc cagcgccagt tggcaacagc gcagacatca ctggatctgc
    25561 ttccgcaata tgatacagat atcgaatccc gggcaaacga actgtggaaa aaggctgtgc
    25621 acactatcaa taacacaacc agggaagaac gccgggtatg ttatatcaca ctgaatcgtc
    25681 cggaagagga acactccggt cctgaagata taccttcagt gaaggcgatt ctgaatactc
    25741 tgctccccct ggagatgctg ttacctgtac cggatctaac tgcatccccg ccccataaaa
    25801 aaaatgtcgc tcagacacca tcaggtggaa aacaagagag tcgtaaaaaa agattctgat
    25861 aacacctgta tggctgattt taataacaac ctttacgcga ttattaatct cctccgctca
    25921 ccatagtaag cggaggattc aggatttcaa tccatcacca gaggtacaat aaatgtgtat
    25981 tagtagcccg gaaacaaact catggtctgt tatttatcgt aaaaattccg gagaagatat
    26041 caatatcact tccctgacgt ttaaaaatag tcttctggca gcccggacac tgatggtgcc
    26101 cgaaaactat atgatatgca tactcagaaa cggggagagg gtcagacgat gggacagaga
    26161 aatacttgca ggctcaaaca gatggtacaa gtgttctccc gataatttcg aaatactggg
    26221 taagctcccc ataataaata aagtcacaaa actgataaaa tcataatcta cggattttaa
    26281 tcagttaagc taaatcagga tgaacgcttt gattatgaat tatacatgaa aacaaatatg
    26341 ggcagcacaa aaaatggtgc cagggttccc cacgttcgta atagattcac ttctatggat
    26401 attggtatga attcccagat cctgccagac atgctgatta attttgccct tattaacatc
    26461 acagacagaa agaacgaggg taccaatacc attgatggta actggcaggc cgatgaagga
    26521 cgccgttacc gggataatgt aagaatctat ttttgagtat gtcaacataa cctgaggcaa
    26581 agtacctcag gttccagtgt agtaatcagc caacctgaca gtcttctgtt agccctacag
    26641 atatgtcgtg gagctaaccg cgcatgtcaa agccggattt ttgaaaatct ctataaactt
    26701 ccggattgtt gaaggccggg aatttagctt tatgggctgc tgactttatc gcttcgcaat
    26761 aggctttatc gccattactg gttgatattt ttaacgccgt gccatcccgg gagaattcca
    26821 tatgcaacct gcattttttc cttttccatt tttgcggatc atcaagttta gcattaattg
    26881 catccctgat tccccgtgct tgctcccccc attcatcctg atcatcccaa cgtcctgaac
    26941 tgcaactacc cagggctgta gttttatggc aatttgaagg atgtaatggc gcacatcccg
    27001 ccacaaagct gactaaaata gccagcataa cgattttctt tacccccata tattgctcct
    27061 caatccatat agatctcagt accatagctc tgtcagagca aagaaaatct taactaatac
    27121 aagttaagat ttttaatatc cactcctcat ccctacattc caggtgatgg tggcgtccac
    27181 tcattatcct gaactggagc ataagatgcg ccatgccctc cggatatgct tttctgttgc
    27241 ctgatatatt cgtggtagta ctgttcacta cgctgacgat gtaaatccac atccgggcgg
    27301 ctgattacaa agtgtccatt tccggttatt ccagctgcat tatgccaggc caccatttcc
    27361 gcaaatacat cattaaactg ttgatatgag ccttccggtt caaaacccat tcctggctca
    27421 cgatcaaaca atacaggagc aatcagattc agcttatcca ccagttcatt tctgtcctga
    27481 tgatggtaga acaccacacg gtccccataa tccacacaga ctaactcgcc attcctccga
    27541 tcacgaaaac gcacactgcc atctttctgc agacgggctt cgagaacgcc agtgtcttca
    27601 taaagtggag ttcctcccgg ttcacagaga atcacgcagc ggtcggcatt cgttgttcgt
    27661 cgcttgtctt tgcgacgatc acgataatcc cagccacgta actgtgataa tgcagctctg
    27721 tctccctgta ccgcctgttg ctccacccac tgccggtagg acaaaggata ccacttaccc
    27781 tccgcaatca gtgacaaccg ctcttccttc acactctcct tcagcctgat cagcgcctgc
    27841 atacgctgca cttctgcaat atggtaatgc agcttacgca attgtggatc ccggaactgc
    27901 acacggatat acgccttgcg ccgacggcag gcagcatgaa tttcccgtaa ccgttctccg
    27961 taacgtaagt cgggtttacg ccagtgctcc ttccatgcaa gataacgcgc gcgcaaatct
    28021 tcccgcgcag ctgcacgggc ttcacggcgt tcacgacgca gtacaggatc gcgccggaag
    28081 ccgggctcca cttcatcgct ggcagcaagt tccggattgt agcgacattc aggcttcacc
    28141 cgttcaaata tatctgcacc tgcaatctca aatggccctg cctgcggctc agccctggat
    28201 aacgtcaaat ccgggtggat actgctggcc ttcactggtg tgagctcatg attaaacgca
    28261 tccacaatca caagaccatg atgctgtttc tgcaacataa gccctttacg cgcaaacatc
    28321 tcatggcacg cctgccagct gaaattcccg cccgcgatca tatcttcaat caacgactca
    28381 cactgaacag tcgtgtaccc ccacagactt tccttatcag aaaacagttc gcgctttatt
    28441 gctccctgtg gacctgtccc catccgcata cgataccatt cgtaatcact gacagcctga
    28501 gtaccaagat tacgtccccg gaatcgttct ccgggtataa cctgagtaaa aatatccttc
    28561 ggtacaggct gccatccacc atccagatcc tgcatataat ccagcgtcat taacggccac
    28621 acactgtctg cacgtactgg tgtctgttgt ttatcaaatc catcatgcag taccagatgc
    28681 ccgtgctgga tccccatcca taatccagcc tttgccagag ttctgtgcac atccaggcaa
    28741 ctttccagct caccatttct cgccatttcc ggtaagcgtt cagcaaagtg acgacaggcg
    28801 tactgtttca gactctccgt ttctgccact ttttccggcc ggatatccac gttaatggct
    28861 tccggctcat aacgaccggg tgtccccacc tgactgaaaa tatctgtcgg tacaggctga
    28921 tattccccca gtttccgccc cagtttttca gctgtccatg acggtccgaa cgaactgagt
    28981 gcaacacctt cccgcgctct gtcccagcca tccttcacca ccagttcccc ttcctgcatc
    29041 gtgatataca gaccatcact ggccagccgt ttatgcaaag ataaccagtc ctgtgcaggc
    29101 tcttcacgca gcccggcaat cgacatctga gcaatatatt ccctgaacgt ctgctttttc
    29161 ccgcgtcgcc aggcattacg gcgctcacgt accagcgctg ttttgcgcac aatacggttg
    29221 ccgggtgcat gcacaaagca gccattatca ggcgcgaatc cgtgcttaag ttcaagttca
    29281 cgacatgcac ggctgagcct ttcctgactc caggaaagac gattaatatg accactaaca
    29341 ggatgaacac gattcactgc gacatgtaca tgcaggttat ccgtgtccgt atgaacggca
    29401 gccacgtact gatgttttga gagttcaagg gatttcaatg tgtgacgcac acagtcaaaa
    29461 agctgctcag gacgcgggct ttcatgcgca ggccaggaga gaatgtagtg gaaaacgggg
    29521 tccgtatcat tacgggaaaa aacagctttg tccgccttgt actgcatctc ttcagcagct
    29581 gtttccagtg aattgcagtt atggaaacag gtaacgccat agaaatttac ccactgactg
    29641 ccgtctttca tcacgtcaat cagggagaca aatttttcgt tacgcaggcg tgtagcatag
    29701 tcgacaaggc gtctgaaacg gttgcggtga gggacgtcac tcttgatttc gctgtcttct
    29761 gtgagctcat tttcctgcat atcatcccgc acagatgtgt acgcaatcag gtcttcaaac
    29821 gaagacctgc cgtcacgtct tttgtgcgga ataatggcat tcatcagcag ttatctcagc
    29881 gtcccattaa gtctcttcgc agagcattga catattcagt aatggcaaca agaacctcgg
    29941 catactcctt atcaccagta cgttttcctt ccacaaacag atgcttctga aggcggccaa
    30001 gacgcataat ctcatcaatc atacggtcgt cagtacggca gttaaccacc ttatccagtg
    30061 aagagcttcg tataaatgtt gaaaccgtct gtccacttga ctccgctttt tctctgacga
    30121 tttcagcctc atcctccgtg aatctcgccg tgaattttac aattctcttc ctgttctcac
    30181 tgcccgttcg ggttttcttc tcactcatgg tctgctcctt accggggtgt cggggcgaag
    30241 ccctgaccag gaggcatttg tctgattgcg cgtgcgcggt ccgacaaatg cacatcctgt
    30301 cccgtttttc aggacattat agcctgttat ttttgtgcat catagtctta tgcagacggc
    30361 agaggcaggg cgcagccctg ccaggagtca gtttttgatg ataaaatcag gcttcaacat
    30421 gaaacagaga gaaagtgcca atggcttcta aaaaatttta ctcagatgat gacatccagc
    30481 tggcaaaagc tgctttatcg gaattgccag atctcaccgc tcaacggaaa acattgcgtg
    30541 attttcttga tgccattcgt gatgacatta ttgtcctggt aaggacaaag ggttatacac
    30601 tggcagatgt tcgtgacacg ctcaaaaatg cagggtatga agtaggagag aaggcacttc
    30661 gtgacattat tcgggaagca gaatcgaaaa aaccatcccg ccgttcttcc tctaaaagag
    30721 cacctaaaaa gacagattca ggcaggaatg atggcatcga tatgaacaac aattgatgtg
    30781 acaatgtcac atcgtgttct gagattatga tgattacaga aaaaaagccc cggcataaat
    30841 accagggctt ttctgtttaa tcagactcgt ggcggcaatg caccaggata atgccgtgca
    30901 ataatatcat tcagtttttc aacaaggtca ggacgcttaa agacaatgtg tacggtccct
    30961 ttctggtagc cacgcaccgt aaaatattcc ccttcaaaca actcaccgac attgtcgccg
    31021 ttcccgagaa attcaccata ctgcgccccg atgctgttcc tgaagtcagg aacgttgcgc
    31081 ccctccagca gataaaacgg acgtaccagg tcatccagtg cctcccgtcc gctgcagtcc
    31141 agcgtcacac gcccgttact ccagcggtac agcaggtttt caataatgat tttctttccc
    31201 agacggcagg gattattggt tctgtaatcc catgacaatt tccggtagac atcaatcagt
    31261 ccatgttcaa atgtctgcat cttactggca ttcagatggc gaaaagttgc cagtacattg
    31321 tccagggtga tttcaggaca ggtgtcgctc atcagctggc tgttccactc atcacgctgg
    31381 catgaactca tgaatgtgta catgccagta tccgtcatca ggcgatccca gattttccgg
    31441 tcggcaagac gggtgatatt cttttttgct tcttccggtt tatccatgca tcgaacggag
    31501 tcagtcacgc agtcttcaaa cccgtagaga aaaatgtcac ctgacgcatc aagcagaatc
    31561 ctgcgtgctg tcgccaggca ttccatcgcc tgcatgaaca gtgaaatacc ttcgttacgc
    31621 tgcgcgatga tgcggtcgat agccacagac ggaataacat ctgtcgtgcc gtcagtattc
    31681 aggtcagcag tgggtaaagt ctgattcatc acattgtctc ctcatcgtca cgactgccgg
    31741 gtcagccagg tcatttcccg gaggagcgaa gctcct
//
LOCUS       DAZGAP010000033        31662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGAP010000033 DAZGAP010000000
VERSION     DAZGAP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.033"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..463
                     /gene="agaB"
                     /locus_tag="ACHLAM_001301"
     CDS             2..463
                     /gene="agaB"
                     /locus_tag="ACHLAM_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO7852861.1"
                     /translation="MLLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDIQQKL
                     MSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKDVNV
                     GNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            502..1305
                     /gene="agaC"
                     /locus_tag="ACHLAM_001302"
     CDS             502..1305
                     /gene="agaC"
                     /locus_tag="ACHLAM_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO7852862.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1295..2086
                     /gene="agaD"
                     /locus_tag="ACHLAM_001303"
     CDS             1295..2086
                     /gene="agaD"
                     /locus_tag="ACHLAM_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO7852863.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPLLKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHIGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2087..2854
                     /gene="agaI"
                     /locus_tag="ACHLAM_001304"
     CDS             2087..2854
                     /gene="agaI"
                     /locus_tag="ACHLAM_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO7852864.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(2904..3767)
                     /gene="rsmI"
                     /locus_tag="ACHLAM_001305"
     CDS             complement(2904..3767)
                     /gene="rsmI"
                     /locus_tag="ACHLAM_001305"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO7852865.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3832..5868
                     /gene="lpoA"
                     /locus_tag="ACHLAM_001306"
     CDS             3832..5868
                     /gene="lpoA"
                     /locus_tag="ACHLAM_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO7852866.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5826..6221
                     /gene="yraN"
                     /locus_tag="ACHLAM_001307"
     CDS             5826..6221
                     /gene="yraN"
                     /locus_tag="ACHLAM_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO7852867.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6241..6831
                     /gene="diaA"
                     /locus_tag="ACHLAM_001308"
     CDS             6241..6831
                     /gene="diaA"
                     /locus_tag="ACHLAM_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO7852868.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6841..7416
                     /gene="dolP"
                     /locus_tag="ACHLAM_001309"
     CDS             6841..7416
                     /gene="dolP"
                     /locus_tag="ACHLAM_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7852869.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7529..8569)
                     /gene="yraQ"
                     /locus_tag="ACHLAM_001310"
     CDS             complement(7529..8569)
                     /gene="yraQ"
                     /locus_tag="ACHLAM_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7852870.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8642..9277)
                     /gene="yraR"
                     /locus_tag="ACHLAM_001311"
     CDS             complement(8642..9277)
                     /gene="yraR"
                     /locus_tag="ACHLAM_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO7852871.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9405..9923
                     /gene="yhbO"
                     /locus_tag="ACHLAM_001312"
     CDS             9405..9923
                     /gene="yhbO"
                     /locus_tag="ACHLAM_001312"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO7852872.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9903..10346)
                     /gene="yhbP"
                     /locus_tag="ACHLAM_001313"
     CDS             complement(9903..10346)
                     /gene="yhbP"
                     /locus_tag="ACHLAM_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO7852873.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10397..10699
                     /gene="yhbQ"
                     /locus_tag="ACHLAM_001314"
     CDS             10397..10699
                     /gene="yhbQ"
                     /locus_tag="ACHLAM_001314"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO7852874.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(10686..11189)
                     /gene="yhbS"
                     /locus_tag="ACHLAM_001315"
     CDS             complement(10686..11189)
                     /gene="yhbS"
                     /locus_tag="ACHLAM_001315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7852875.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11183..11707)
                     /gene="ubiT"
                     /locus_tag="ACHLAM_001316"
     CDS             complement(11183..11707)
                     /gene="ubiT"
                     /locus_tag="ACHLAM_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO7852876.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11916..12911
                     /gene="ubiU"
                     /locus_tag="ACHLAM_001317"
     CDS             11916..12911
                     /gene="ubiU"
                     /locus_tag="ACHLAM_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO7852877.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12920..13798
                     /gene="ubiV"
                     /locus_tag="ACHLAM_001318"
     CDS             12920..13798
                     /gene="ubiV"
                     /locus_tag="ACHLAM_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO7852878.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13879..14886
                     /gene="yhbW"
                     /locus_tag="ACHLAM_001319"
     CDS             13879..14886
                     /gene="yhbW"
                     /locus_tag="ACHLAM_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO7852879.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTPMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15004..16248)
                     /gene="mtr"
                     /locus_tag="ACHLAM_001320"
     CDS             complement(15004..16248)
                     /gene="mtr"
                     /locus_tag="ACHLAM_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO7852880.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(16402..18291)
                     /gene="deaD"
                     /locus_tag="ACHLAM_001321"
     CDS             complement(16402..18291)
                     /gene="deaD"
                     /locus_tag="ACHLAM_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO7852881.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18284..18364)
                     /gene="yrbN"
                     /locus_tag="ACHLAM_001322"
     CDS             complement(18284..18364)
                     /gene="yrbN"
                     /locus_tag="ACHLAM_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO7852882.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18471..19355)
                     /gene="nlpI"
                     /locus_tag="ACHLAM_001323"
     CDS             complement(18471..19355)
                     /gene="nlpI"
                     /locus_tag="ACHLAM_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO7852883.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19464..21599)
                     /gene="pnp"
                     /locus_tag="ACHLAM_001324"
     CDS             complement(19464..21599)
                     /gene="pnp"
                     /locus_tag="ACHLAM_001324"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO7852884.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21846..22115)
                     /gene="rpsO"
                     /locus_tag="ACHLAM_001325"
     CDS             complement(21846..22115)
                     /gene="rpsO"
                     /locus_tag="ACHLAM_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO7852885.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22264..23208)
                     /gene="truB"
                     /locus_tag="ACHLAM_001326"
     CDS             complement(22264..23208)
                     /gene="truB"
                     /locus_tag="ACHLAM_001326"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO7852886.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23208..23609)
                     /gene="rbfA"
                     /locus_tag="ACHLAM_001327"
     CDS             complement(23208..23609)
                     /gene="rbfA"
                     /locus_tag="ACHLAM_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO7852887.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23773..26445)
                     /gene="infB"
                     /locus_tag="ACHLAM_001328"
     CDS             complement(23773..26445)
                     /gene="infB"
                     /locus_tag="ACHLAM_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO7852888.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26470..27957)
                     /gene="nusA"
                     /locus_tag="ACHLAM_001329"
     CDS             complement(26470..27957)
                     /gene="nusA"
                     /locus_tag="ACHLAM_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO7852889.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(27985..28437)
                     /gene="rimP"
                     /locus_tag="ACHLAM_001330"
     CDS             complement(27985..28437)
                     /gene="rimP"
                     /locus_tag="ACHLAM_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO7852890.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28644..28720)
                     /locus_tag="ACHLAM_001331"
     tRNA            complement(28644..28720)
                     /locus_tag="ACHLAM_001331"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28684..28686),aa:Met,seq:cat)
     gene            29068..30411
                     /gene="argG"
                     /locus_tag="ACHLAM_001332"
     CDS             29068..30411
                     /gene="argG"
                     /locus_tag="ACHLAM_001332"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO7852891.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30419..>31662)
                     /locus_tag="ACHLAM_001333"
     CDS             complement(30419..>31662)
                     /locus_tag="ACHLAM_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7852892.1"
                     /translation="VIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDAKNKKAF
                     SPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDTYVLIVG
                     ESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTADSVLSHD
                     IHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFDELLLPH
                     LSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIHYTDSLL
                     GQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWYSPVLGD
                     GVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDANHDVFDY
                     VMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         7568 a         8579 c         7962 g         7553 t
ORIGIN      
        1 tattctctta acccgtattg ataaccgtct ggttcacggt caggttggcg tgacctggac
       61 atccaccatc ggtgcgaatc tgctggtagt cgtggatgat gttgtcgcta acgatgatat
      121 tcaacagaaa ttaatgagta ttaccgcaga aacctacggc tttggcattc gtttctttac
      181 tatcgaaaaa accattaacg tcatcggcaa agctgcaccg catcaaaaga tcttcctgat
      241 ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt ggtattgacc tgaaagatgt
      301 caacgtcggc aatatgcatt tctcggaagg gaaaaagcaa atcagcagta aagtttatgt
      361 cgatgaccag gatctcacgg acttacgttt tattaaacaa cgtggcgtga atgttttcat
      421 tcaggacgtc cctggcgatc aaaaagaaca aatccctgac taaatctaaa atcgccttaa
      481 tattggtttg aggtaataaa aatgcatgaa ataaccctac ttcagggatt atccctggcg
      541 gcgttagttt tcgttctggg gattgatttt tggctggaag ccttattttt attccgcccg
      601 ataatcgttt gtaccctaac tggcgctatt ctcggtgata ttcagactgg cttaattacc
      661 ggtggtctga cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc
      721 aacccgatta tggcgggtct gatgaccacc gtcattgcat ggtctacggg cgttgatgcc
      781 aaaacagcaa ttggtcttgg cctgccgttt agtttgttaa tgcagtatgt cattctgttc
      841 ttctattctg ctttctcatt atttatgacc aaagccgata aatgcgcgaa agaagcggat
      901 acggcagcgt tttcccgact taactggaca acgatgctca tcgttgcttc agcgtatgcg
      961 gtgattgctt tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg
     1021 atgcccgcct ggctgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt
     1081 tttggcttgc tgctgcgcgt gatgttcaaa gcgcaatata tcccttacct gatcgccggc
     1141 ttcctgtttg tttgctacat ccaggtcagc aacctgttgc cggttgccgt actgggcgca
     1201 ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc
     1261 gttgccagta aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga
     1321 tatcacccgt ctgggctttc gttcgtcact gctacaagca agctttaact acgaaaggat
     1381 gcaggcgggc ggctttacct gggcgatgtt gccgctcctg aaaaagattt ataaggacga
     1441 caaaccaggc ctaagcgcgg cgatgaaaga taacctagaa tttattaata cccacccgaa
     1501 tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gagaaagggg aaaaccgcga
     1561 caccattaaa ggcctcaaag tggcgctgtt tggcccaatc gccgggattg gcgatgcgat
     1621 tttctggttt accttgttac caattatggc ggggatttgc tcatcgtttg ccagtcaggg
     1681 aaacctgctg gggccgattc tgttctttgc cgtttacctg cttatctttt tcttgcgcgt
     1741 cggctggact cacatcggtt actcggtcgg cgtgaaggct attgataaag tgcgagagaa
     1801 ctcgcagatg attgcccgtt cggcaaccat cctcgggatc acggtaatcg gcgggctgat
     1861 cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt
     1921 cgcgctgcag caggatttct tcgataaagt cttcccgaac attttaccga tggcctacac
     1981 cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt
     2041 gacgtttgtt ctttctattg tttgttctgc attcggcatt ttgtaaatgg aacgaggcac
     2101 cgcgtctggc ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcaacaagt
     2161 tgaaaactat acggcgttaa gtgaacgtgc cagcgaatat ttattggccg tgatccgtag
     2221 caaaccggat gccgtgattt gcctggcgac cggagccacg ccattactga cgtatcatta
     2281 tctggtagaa aaaatccacc agcagcaggt tgatgtcagc cagctcacct tcgtgaagct
     2341 cgacgaatgg gtggatctgc cattaacgat gcctggtacc tgcgaaactt tcctgcaaca
     2401 gcatatcgtg cagccgctgg ggctacgtga agaccagctc atcagctttc gctccgaaga
     2461 gattaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga
     2521 tttatgcgtt ctcgggttgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaag
     2581 cctgcaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt
     2641 aaaaaccgcg ggccgtcctg tgactcgtgg gatcacctta ggcctgaagg atattctcaa
     2701 tgcccgcgaa gttttgttac tggtgactgg cgaaggaaag caggatgcga cagaacgttt
     2761 tctcacggct aaagtctcta ccgctatccc ggcttcattt ttatggctgc ataactattt
     2821 cacatgcatg attgatgaga tgtgtcgtcg ttaactcgcc tgctacaggc gttgcaacgc
     2881 tattcctgct gcgacgcctg taatcactct ccctgctgct ccagcgcata cttatacagc
     2941 gcatttttct tcacgccgtg aatctctgcc gccagcgccg ccgctttttt cagcggcagt
     3001 tctgcctgta gcagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgcgct
     3061 ttatggcctt cgacaatcag caccatttcg cctttgcgac ggttttcatc ttctttgacc
     3121 cacgccagca gctcgccaac gggcgcgccg tgaatggttt cccaggtttt ggtcagctca
     3181 cgcgccagaa ccacgtagcg ggattcgcct aataccgcaa cgatatcttc caggctatct
     3241 aacagacggt gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc aatagctttt
     3301 agcgcatcac ggcggccttt tgatttggca ggtaaaaagc cttcataaca aaaacgatcg
     3361 gatggtaaac ccgcggcgct taacgcagtg atagcagcac acggccctgg tagcggcacc
     3421 acgcgaatcc ccgcttcacg acaggtacgc accagatggt agccaggatc gttaattagc
     3481 ggcgttccgg catcggaaac cagcgcaatg ttttgcccct cttgcagttt cgccagcagc
     3541 gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa acaaccgggc attaatccca
     3601 aaatgttgca gcaataaacc ggtgtgacgc gtatcctcgg cggcaatcag atcaacggcc
     3661 tgtaatacct ctaatgcacg ctgggtgata tcagccagat tgccgattgg cgtcggtaca
     3721 atgtaaagct ggccctgaga attatccgcc gattggtgtt gtttcattgt gtcgtccgta
     3781 ttgccgattt aatattgagc attgcgtaaa aaaaatatca ctggatacat tatggtaccc
     3841 tcaacatttt ctcgtttgaa agccgcgcgt tgtctgcctg ttgttctggc agccctgatt
     3901 ttcgccggtt gtggcaccca tactcccgat cagtccactg cttatatgca gggcacggcg
     3961 caggctgatt ctgcctttta cctgcaacag atgcagcaaa gttctgatga taccaggatc
     4021 aactggcaat tactcgccat tcgtgcactg gtgaaagaag gtaaaaccgg acaggcggtg
     4081 gagttgttta accaactacc gcaagaactg aacgattctc agcgtcgcga gaaaacactg
     4141 ctggcggcag agattaaact ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg
     4201 aaaatcacac ctgccgattt agaacaaaac cagcaagccc gttactggca ggcaaaaatc
     4261 gatgccagcc aggggcgtcc ttccattgat ttactgcgcg cgttaattgc tcaggaaccg
     4321 ctgctcggtg cgaaagaaaa gcagcagaat attgacgcca catggcaggc gctctcctcc
     4381 atgactcagg agcaggcgaa tacgctggtg atcaacgccg acgaaaatat tctgcaaggc
     4441 tggctggatt tgcagcgcgt ctggtttgat aaccgtaacg atcctgacat gatgaaagct
     4501 gggatcgccg actggcagaa acgttatccg aacaacccgg gcgcgaaaat gctaccaacg
     4561 cagttggtta acgtaaaagc gtttaaaccc gcatcgacca acaaaatcgc cctgctgttg
     4621 ccgctgaatg gccaggcagc ggtatttggt cgcactattc agcaaggctt tgaagcggcg
     4681 aaaaatatcg gcactcagcc agtggcggcc caggtagcag ccgcacctgc cgcagacgta
     4741 gcagaacaac ctcagccgca aaccgtggat ggcgttgcca gcccggcaca agcttcggtt
     4801 agcgagctga ccggtgaaca gcctgcggcc cagccggtac ctgtaagcgc cccggcgaca
     4861 agcaccgcag cggtaagcgc acccgcaaat ccatccgcag agctgaaaat ctacgatacc
     4921 tcatcacaac cacttagcca gatcttaagc caggttcagc aggatggtgc gagtattgtg
     4981 gtcggtccgt tgctgaaaaa taacgttgaa gagttgctga agagcaacac cccgctgaac
     5041 gtgctggcac tgaaccagcc ggagaacatc gaaaaccgcg tcaatatttg ttacttcgcg
     5101 ctttcaccgg aagacgaagc gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa
     5161 gcgccgctgg tgctgatccc acgcagttca ttgggcgacc gcgtagccaa tgcgtttgcg
     5221 caagagtggc agaaactggg cggcggcacc gttctgcaac aaaaatttgg ttccaccagc
     5281 gaattacgcg cgggtgttaa cggcggttct ggtattgctt taacgggtag cccgattact
     5341 cccagagcga caaccgactc cggcatgacg accaacaatc caacgctgca aaccacgcca
     5401 accgatgacc agttcaccaa taatggcggt cgtgtcgatg cggtgtacat tgtggcaacg
     5461 ccgggtgaaa tcgcttttat caaaccgatg atcgccatgc gtaacggtag ccagagcggt
     5521 gcaacgctgt acgccagttc ccgcagcgca caaggcaccg caggcccgga tttccgtctg
     5581 gagatggaag gcttgcagta tagcgaaatc ccgatgctgg caggcggtaa tctgccgtta
     5641 atgcagcagg cgctcagcgc ggtgaacaac gattattcgc tggctcgcat gtatgcgatg
     5701 ggcgtcgatg cctggtcgtt ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt
     5761 gaaatcaacg gtaataccgg aagtctgacg gctaacccgg attgcgtgat taacaggaag
     5821 ttatcatggc tacagtacca acaaggtcag gtagtccccg ccagttaacc accaaacaga
     5881 ccggcgatgt gtgggaagca caagcgcgtc gctggctgga aggcaaagga ctgcggttta
     5941 tcgccgctaa cgtgaacgag cgtggcggcg agatcgatct gataatgcgt gaaggccgga
     6001 tcaccgtttt tgtcgaggtg cgttaccgcc gctctgcgct ttatggcggc gcggcagcca
     6061 gtgtgacccg cagcaaacaa cacaaattat tacagactgc ccgcttgtgg ctcgcgcgtc
     6121 ataatgggag ttttgatact gtggattgcc ggttcgatgt ggtagccttc accgggaatg
     6181 aggttgagtg gattaaggat gcctttaatg accactcata attaaggttt aaggattagc
     6241 gtgcaagaaa gaattaaagc ttgcttcact gaaagcattc aaactcaaat tgcggcggca
     6301 gaggcgcttc cggatgccat ctcgcgtgca gccatgacgc tggttcagtc tctgctcaat
     6361 ggcaacaaaa tcctctgttg tggtaatgga acttccgctg ccaatgcaca gcattttgct
     6421 gccagcatga tcaaccgttt cgaaacggag cggcccagct tacctgccat tgcactaaat
     6481 actgataatg ttgtcttaac ggcgattgcc aacgatcgct tacatgatga agtgtatgca
     6541 aaacaggtgc gggcgctggg tcatgcggga gatgtattgt tagccatttc cacccgtggc
     6601 aacagccgcg atattgtaaa agcggtagaa gctgccgtta cgcgtgatat gaccattgtg
     6661 gcattaaccg gctatgacgg cggtgaactt gcaggtttgt tagggccaca ggatgtggag
     6721 atccgcattc cttcgcatcg tagtgctcgc attcaggaaa tgcatatgct gacggtaaat
     6781 tgcctgtgcg atctgatcga taacacgctt ttccctcacc aggatgttta aggagaatac
     6841 atgaaggcat tatcgccaat cgcagtcctt ctttccgcgc tgctgttgca aggttgtgtt
     6901 gccgctgccg tagtgggtac cgctgctgta ggtaccaaag ccgcaactga cccacgcagt
     6961 gtcggcaccc aggtggacga tggtaccctg gaagtgcgcg tgaacagcgc attgtcgaaa
     7021 gacgaacaaa ttaagaaaga agcgcgcatt aatgtcacgg cctatcaggg caaagtgctg
     7081 ctggttgggc agtcaccaaa cgctgaactt tcggctcgcg ccaaacagat tgctatgggc
     7141 gtagacggtg ccaacgaagt gtataacgag attcgtcagg gccagccgat tggtctgggc
     7201 gaagcgtcta acgatacgtg gatcaccacc aaagtacgtt cgcagctgtt aaccagcgac
     7261 ctggtgaaat cgtccaacgt gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg
     7321 ctggtgactg aacgtgaagc gaaagcggcg gcagatattg ccagccgggt aagcggcgtg
     7381 aagcgggtaa ctacggcgtt tacgtttatt aaatagcagc ccttgtaatg cctgatgcga
     7441 cgcttgcagc gtcttatcag gcctacaacg atgcaaaccg taggtcggat aaggcgttta
     7501 cgccgcatcc gacatgtttt ccctcaaatc aaaccaacag cgccagcccg ccgacaatca
     7561 caccagacac tgccaccatc gcccccgtca gccataaggc tttcgccggg aacgctttgc
     7621 gcagcataat cagtgacggc aaactcaccg ccgggagcgt catcaacaat gccagcgccg
     7681 gagcagttcc catacctgcc agcatcatcg tttgtacaat cggaatttct gctgccgtgg
     7741 gaatgacaaa caagcatccc gctaccgcca tcgccaccac ccacatcagg ctgttatcga
     7801 cagcaccatc ggcatggggg aataaccaga cgcgagcggc acccaacacc agtactgcaa
     7861 ggatgtaaac cgggatcgta ctccagaaaa gcgtccatag cgccctgccc cagcggctaa
     7921 aaaatcctcc ctgtgcttcc ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt
     7981 cacgcaccca tttttgcacc agcgtcgcaa tcagcaacac catcaccagc cccgccacca
     8041 gacgaatcgc cgcaaaaccc cagccgagga cgaaccccat aaacaccagc gtcgccgggt
     8101 ttaacaacgg attccccatc cagaatgcca gcgcaccgcc catcgacacc tgttggcgac
     8161 gcattcccgc cgcgaccgga gccgcacagc aggtacacat catgcccggc agcgaaaaca
     8221 gcgttcccag cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaat aaccagtcac
     8281 gcgggatcag cacctgaatc aacgacccga gcagtacgcc cagcaccgcc gctttccata
     8341 ccgcgaggaa atagatcatc gcgtaatcca acgccgcctg ccacggatta gcatccgcct
     8401 gcgcaaggat agatttaccg atactgtggg tttcggcagc agtaaacgct ttgccgtagt
     8461 aaggttgcca tttcacatac cagagaccag caatgacaac gagaaagaaa agcgcgggtt
     8521 tccaccactg aatgggcgtt gccgcctgag atgaagactg accagtcata gcattcccca
     8581 ggaataagtt atgataattg agcgcgtgaa tattacgctc actatcaatt cttgggaata
     8641 attattcagc tcttttgcgt aattctgaag agcttaaaat cgtcacacct tcatgctcag
     8701 gccgcatcgc ctctgccagc atgacccgcg ccacgtctct cgcatcaatg gatttccagt
     8761 tacccggtaa caaacggaac agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac
     8821 gatcgcccag taacatcgac gggcgagcaa tggtcaattt cggccagttc tgggcgatta
     8881 atgcttcttc catctcccct ttgacgcggt tatagaaaaa cggcgagtgg gcattagcac
     8941 ccatagcact gaccaccagc atatgctgcg caccgagccg ccgcccggtt aatgcggtat
     9001 ccaccaccag cgtgtaatcg gcatgaataa acgcctcttt actccccgct tctcgccgcg
     9061 tggtgccgag acaacaaaac acaatgtcga tgggatcggt gacctgcgcc agcgcgtcgg
     9121 tcagttgcgg atcatgggga ttaaacacac cgggcatatc gcccaacgga cgtcgcgtcg
     9181 gcgcggctat ggcgttaact ttcggttcat taatcaacat ccgcagcagg tgaccaccca
     9241 ccagccccgt tgcgcctgta atcagtacct ggctcatctt cgctccttta cagaattgtc
     9301 tgccttgcgc tccccggctc atgcaccagg cttaatagtc tacgaggtaa ttatccccga
     9361 ttgtgggaaa ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt aagaaaattg
     9421 ccgttttaat cactgatgaa tttgaggatt cagaatttac ttcacccgca gacgagttcc
     9481 gtaaagccgg acacgaagtg attaccattg aaaaacaagc gggtaaaacg gtgaaaggca
     9541 aaaaaggaga agccagcgtg gccatcgata aatccatcga tgaagtgacg ccagcggagt
     9601 ttgatgccct gctgctaccg ggcggccatt caccggatta tctgcgtggc gacaaccgtt
     9661 ttgtcacttt cacccgtgat tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg
     9721 gtccgcagct gctgatcagc gccgatgtga ttcgggggcg caaactgact gccgttaaac
     9781 cgatcattat tgatgttaaa aacgcgggcg cggaatttta cgatcaggaa gtggtggtcg
     9841 ataaagatca gctggttacc agccgtacac cggacgatct gccagcgttt aaccgcgagg
     9901 cattacgcct gctcggtgcc tgagttacgt agccagatca tttttttacc aaagcccagc
     9961 gtgttgtcgg tgaatttgat ttcatcgagc cggatttccc acaccggggc cgacagcatt
    10021 ctggcaaccg gaaagcggcg attgtacgcc tggcgcgcaa ggtcgctttc ctcaccttca
    10081 agcctgcgga tctcgccttt aaattgcacg ccgcgaatta acgctaccgt tttcggctga
    10141 ccgtttaccg ttccggcaac tgccgcctgc ggcccactca tctgcgcgtg gcgcgttttt
    10201 tcttccgtca aaatgtagaa ggcgactttc tgcgcatcaa aaagataaaa ggcattagcg
    10261 caccagagtt ccccttcctg ctgcacacac caggtgacaa catgttgttt tgccagccaa
    10321 cggctgatgg cggtgagtgt ttccattgct gttctctttt atactgtggg cctgacctta
    10381 acatgcgacg cagacgatga caccctggtt tctttacttg atccgtaccg ccgacgataa
    10441 gctttatacc gggatcacca cagatgtcga acgccgctat cagcagcacc aaagcggcaa
    10501 aggggcgaaa gcactgcgcg ggaaaggaga actaacgctg gcgttttccg cgccagtggg
    10561 cgatcgttcg ctggcactac gggcggaata tcgcgttaag caactgacca aacggcaaaa
    10621 agagcgtctg gtagcggaag gcgcagggtt tgcagaactg ttgagcagcc tgcaaacccc
    10681 aaagattaaa agcgattgaa gtgctcgtga tactcaacca gaccagttac accgttcagc
    10741 gcgtcatccg ctaaacgatg tacctggaag gcactttcag tgcccggcca gcggcaacgc
    10801 agatcgtgat gtgccgccag ttcaaagccg aaacggctgt acagcgccgg atcgcccagc
    10861 gtcaccactg cggcatagcc gaactcatta agcgaatcga gtccttcata gaccagttgg
    10921 cgtgccagcc cctgtccacg gtatttttca tctaccgcca gcggtgccat gccgacccat
    10981 tgcaggtctt cgccctgcac atcaaccgga ctaaatgcca catagccaat gacctgacct
    11041 tcgtcatctg tcgccaccag ccccagcgtc agaaagccat cttcacgcag atcgtgaacc
    11101 agcttcgctt ccgcatcact ttcgaatgaa cgacgcagca gggcatcaat acccggcgca
    11161 tcaatgggaa tttctactcg aattagcatg gttcacctac cgatgtctgt ttggtttcag
    11221 gcgcggtttt cattcccgcc tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg
    11281 ctttcggcat ttgctccagt tcaatggcgt ccatcaggtt tttcacatac agccccagct
    11341 ccgtatcgcc ttcaattacc agccgacgct ggaagaagag cgtatccgga tcttgtttac
    11401 gcgccgcaat catcagcaga tcgctggcgt cggcactaaa actcacatca gcttgcgcgt
    11461 tctggctaac gaccagtttg ccattcacca ccgaggtaaa ccattgcagg tcaatatcac
    11521 gcacatgaat acttaaccag cggccttcaa gaaactccag ctcgccatca tccaacgcct
    11581 ggcggaattg ccagcttaag acctgctcaa gaacctggcg ttttagcgca aatggcgtca
    11641 gttttaccgg tacactcaac agagatggcc ccaaatgcac aatacgggaa cgcagtttat
    11701 ccaacacgag ttttactccc tgtttcaaca atcatcctat tttgccatat cagaaaaata
    11761 acatagcggt ataaatcaac aattccatat gaaattgctg ctaccaccaa tacaacttta
    11821 actgccttaa atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt
    11881 tttgcgcttt gatagcgcaa ccttcaggaa aaattatgga gctgctctgc cctgccggaa
    11941 atctcccggc gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat atcgggctaa
    12001 aagatgatac caatgcccgt cacttcgccg gccttaactt taccgagaaa aaattgcagg
    12061 aagcggtgag ttttgtccat caacatcgcc gcaaacttca catcgcgatt aacacttttg
    12121 cgcatccgga cggttacgcc cgctggcagc gcgccgtgga tatggcggcg cagttgggtg
    12181 ccgacgcgct gatcctcgcc gacctcgcca tgctggagta cgccgccgag cgttacccgc
    12241 atattgagcg tcatgtgtca gtgcaggctt cggcgaccaa tgaagaggcg attaactttt
    12301 atcatcgcca ttttgacgtt gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg
    12361 tgaaacaact ggcacgggtc acacctgtcc cgctggaagt gtttgctttc ggcagcctgt
    12421 gcattatgtc ggaaggccgt tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca
    12481 ccgtgggcgc gtgttctccg gcccgtttcg tgcgctggca gcaaacgccg cagggactgg
    12541 aatctcgcct gaacgaagtg ctgatcgacc gttatcagga tggcgaaaac gcaggttatc
    12601 cgacgctgtg taaagggcgt tatctggtgg acggcgagcg ctatcacgcg ctggaagaac
    12661 ccaccagtct caataccctg gaactgctgc cggagttaat ggcggcgaat attgcttcgg
    12721 tgaaaattga aggccgccag cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc
    12781 gtcaggctat cgaccgttgt aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga
    12841 tggagacgct cgggtcgatg tccgaaggca cgcaaactac ccttggcgcg tatcaccgta
    12901 aatggcagtg agaaaagcaa tgaaatattc cttagggccg gtgctatggt actggccaaa
    12961 agagacgctg gaagactttt atcagcaggc cgccgccagc agcgcagacg tgatttatct
    13021 tggtgaagcg gtctgtagca aacgccgggc aaccaaagtt ggcgactggc tggagatggc
    13081 aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc
    13141 gtcctccgaa cttggcgaac tgaaacgtta tgttgagaac ggtgagtttc tgattgaagc
    13201 cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca
    13261 cgcgctgaac tgctacaacg cggtgacact gaaaatattg ctcaaacagg gcatgatgcg
    13321 ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga
    13381 tgagctgggc attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctgccgct
    13441 ggcctactcc gcccgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga
    13501 aacctgctgc attaagtatc cgaacgggcg caacgtgcta tcgcaggaaa accaacaagt
    13561 gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct
    13621 ggcatccatg caggggctgg tcgatgtggt ccgcctgtca ccgcagggta ctgacacttt
    13681 cgcgatactc gacgccttcc gcgccaatga aaatggcgct gcaccactgc cgttgacggc
    13741 aaacagcgat tgtaacggct actggcggcg gctggccggg ctggagctgc aagcctaagt
    13801 aaatagctca ctttgttaac aactttaact actctttaat gcagtattaa agattaatca
    13861 gtaacaaagt gagctgttat gactgataaa accattgcgt tttcgctact cgatctggcc
    13921 cccattcccg aaggctcttc agcgcgggaa gcgttctccc actctctcga cctcgcccgt
    13981 ctggctgaaa agcgcggcta tcatcgctac tggctggcgg aacaccacaa tatgaccggc
    14041 attgccagtg ctgcaacatc agttttgatt ggctatctgg cggcgaatac cactacgctg
    14101 catctggggt ctggcggcgt gatgttgcct aaccactcac cattggtgat cgccgaacag
    14161 ttcggcacgc ttaatacgct ctatccgggg cgaatcgatt tggggctggg tcgtgcgccg
    14221 ggtagtgacc agcgaaccat gatggcacta cgtcgtcata tgagcggtga tattgataat
    14281 ttcccccgcg atgtcgcgga gctggtggac tggtttgacg cccgcgatcc caatccgcat
    14341 gtgcgcccgg taccaggcta tggcgagaaa atccccgtgt ggttgttagg ctccagcctt
    14401 tacagcgcgc aactggcggc gcagcttggt ctgccgtttg cgtttgcctc acacttcgcg
    14461 ccggatatgt tgttccaggc gctgcatctt tatcgcagca acttcaaacc gtcggcacga
    14521 ctggaaaaac catacgcgat ggtgtgcatc aatattatcg ctgccgacag caaccgcgac
    14581 gctgaatttc tgtttacccc aatgcagcaa gcctttgtga aactacgccg tggcgaaacc
    14641 ggacaactac cgccaccgat tcaaaatatg gatcagttct ggtcaccgtc tgagcagtat
    14701 ggcgtgcagc aggcgctgag tatgtcgttg gtaggtgata aagcgaaagt gcgtcatggc
    14761 ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta tggttaacgg gcagattttc
    14821 gaccaccagg cacgcctgca ttcgtttgag ctggcgatgg atgtgaagga agagttgttg
    14881 gggtagtgtg ttttaacgta ggaagcctta tccgagatgg caacgctgtc ctacgtaggc
    14941 ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc
    15001 tgattactga tacaccggca gcaaattaaa gctcgataag atatggacca gtgcgttgcc
    15061 aacgccaaat accagaatca gcataatcat cggcttgccg ccccagacgc ggaatttcgg
    15121 gctaccaaag cgtttacgcg atgcgcgggc caacagcgcc ggaacaattg ccgcccagat
    15181 ggtagccgct aagccagcat aaccaatggc gtacaggaat ccgttaggaa acagcaggcc
    15241 acccacaaca ggcggggcaa aggtcagcaa cgctgttttc aaacggccca tagccgagtc
    15301 gtcgaaacca aacagatctg ccagatagtc aaacaaaccc agcgttacgc caaggaacga
    15361 actcgctacc gcaaagtttg agaacacgac cagcagaaga tccaggctac ggctgttcag
    15421 cacgccgctt aatgcctgta ccagcacatc aatattaccg cccttctcgg cgatgccgat
    15481 aaactccgga cgagggatgt tgcccatcgt cgccagcaac cagatggtat acagcgccag
    15541 cgccatcagc gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata
    15601 cttcatcagg ctcggcacgt taccgtgata accaaacgat gccagacaga atggcagtgt
    15661 catcagcaga tacggcgcat aagacgcatt gctttcggcg acgttgaaca aggtcgttgg
    15721 ctgtacatgc cccagcaggc tgccgaaggt gaggaagaaa gtaatgactt tcgcccccag
    15781 cacaattgct gtcatccggc tgacagcttt cgtactcaac cacaccacga acgctaccag
    15841 cagtgcaaaa ccaaatccag ctgcccgtgc agggacgttc agcgacatct ccgcgaaggt
    15901 gtgatgcaga atcgaaccgc tggcagaaat ataggcgtaa gtcaggatat agagcacaaa
    15961 ggcaatggaa atgccgttga ctacgttcca gcctttgccc agcaaatctt tggtgatggt
    16021 gtcaaagcta gaaccgattc gataattcag gttagcttcc agaatcatca agccggaatg
    16081 cagcatacag aaccaggtaa agatcagcgc cgccattgac cagaagaacc acgccccgga
    16141 catgaccaca ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac
    16201 gccgccaagc agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct
    16261 ccagtgaaaa atagtgcgac tgcgttgtta tgcattgcac tgtaccagta cacgagtaca
    16321 aaagacagaa aaaaagcccc gatggtaaaa atcggggctg tatatattat tttacagatt
    16381 gtgttcgctg ttcagcgatg attacgcatc accaccgaaa cgacgacgac cggtagagtc
    16441 atcacgacgc ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac catcaccacg
    16501 accaccttca cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt cgccactgaa
    16561 accacgacca ccgccacgac gctcaccgcc agtatgcggc tgtgcatcgc ccagtaactg
    16621 catgttcatc ggcttgttga gaatgcgagt gcgcgtaaag tgttgcagca cttcacccgg
    16681 catacctttc ggcagttcga tggtggagtg agaagcaaac agcttgatgt taccaatgta
    16741 acggctgctg atgtcgcctt cgttagcaat cgcaccaacg atatgacgaa cttcaacacc
    16801 atcatcgcgg cccacttcaa tgcggtacag ctgcatatcg ccaacatcac gacgttcacg
    16861 acgcggacga tcttcacggt caccacgcgg gccacggtcg ttacgatcgc gcggaccacg
    16921 gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag
    16981 agtacgttca ccctgtgcca ttttcagcag tgcggcggcc agagtttcga gatccagctc
    17041 ttcaccttct gccgtcggct gaattttgct cagcagtgca cggtattgat ccagatcgct
    17101 gctttccagc tgctgctgta ctttagcggc gaatttttcc agacgacgtt tgcccagcag
    17161 ttctgcgttc ggcagttcaa cttccggaat ggtcagcttc atggtgcgtt caatgttacg
    17221 cagcagacga cgctcacggt tctcaacgaa cagcagcgca cggccagcac ggcccgcacg
    17281 accagtacga ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac
    17341 taccaggctg atacgctcaa cgtccaggcc acgggccgca acgtcggtcg caatcaggat
    17401 gtccagacga ccatctttca gtcgttccag tgtctgctca cgcagcgcct ggttcatgtc
    17461 accgttcagc gcggcgctgt tgtagccgtt acgctcaagg gcttctgcca cttccagagt
    17521 cgcgtttttg gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg
    17581 taccagtgct tcgtttttac gcatacccca gacagtccag tagctctggc tgatgtcagg
    17641 acgggtagtc acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt
    17701 aatgcgacga atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg
    17761 gatctgcgcc ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc
    17821 ttcatccaga accagaccgc tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc
    17881 cagcagacgg cccggagtac cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg
    17941 cacgtcataa cgttggccgc cgtacagggc aaccacgttt acgccgcgca tgtgtttaga
    18001 gaaatccgtc attgcttcag caacctgtac cgccagttcg cgggtcggtg ccagcaccag
    18061 aatctgtggt gctttcagct caggatcaag attctgcagc agaggtaaag agaatgctgc
    18121 agtttttccg ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg
    18181 aatacactct gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag
    18241 gataggagcc ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt
    18301 gtgcctcaaa attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt
    18361 tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc
    18421 cgttaaggtg atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg
    18481 tccgattctg ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat
    18541 cggtgctcaa caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg
    18601 ctgtccaaat cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga
    18661 tgctcagcga gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt
    18721 tcgctaatgt tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat
    18781 ttttcgaagt gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg
    18841 agataaagcc acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga
    18901 tcatcttgcg ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc
    18961 gcgtagttgt aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca
    19021 aaattgcctg cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt
    19081 cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca
    19141 tacaacactc cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg
    19201 gcaaggattt gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt
    19261 accgcgagga cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct
    19321 gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg
    19381 aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc
    19441 gcagcggagg gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct
    19501 gcagcaggtt gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga
    19561 tcaacttcca gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc
    19621 tcaacgcgtt tgtcagcgat ctgagagatg tgtaccagac cttctttacc gccgccgatg
    19681 gcaacaaatg cgccaaagtc aacgatacgg gtcactttac cattgtagac gcggcccact
    19741 tcgatttctg cagtgatctc ttcgatacga cgaatagcat gtttcgcttt ctcgccgtcg
    19801 gtcgctgcga ttttcacagt accgtcatct tcgatttcga tggtggtgcc agtttcttcg
    19861 gtcagagcac ggataacaga accgccttta ccgataacgt ctttgatctt gtccgggttg
    19921 atcttgatgg tatggatacg cggagcgaac tcagagatat cgccgcgcgg cgcgttgatc
    19981 gcctgttcca ttacgcccag gatatgcaga cgcgcacctt tagcctggtt cagcgcaacc
    20041 tgcatgatct ctttggtgat accttcaatt ttgatatcca tctgcaatgc agagataccg
    20101 tcacgggaac ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg
    20161 tcagacagta caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg
    20221 gcagctttga tcggcacacc tgcgtccatc agcgccagag acgcgccgca cacggaagcc
    20281 atagaagagg aaccgttgga ttcggtgatt tcagacacaa cacgtacggt atacgggaat
    20341 ttgtccatat ccggcatgac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt
    20401 tcacgacgct tcggagaacc gaccatgccg gtttcgccta cagagtacgg agggaagttg
    20461 tagtggaaca ggaaggtgtc agtacgttcg cccatcagtt catcaagaac ctgcgcgtca
    20521 cgtgcagtac ctagcgttgc ggtaaccagc gcctgagttt caccacgggt gaacagcgca
    20581 gaaccgtgag tacgcggcag cacaccagta cgcacatcca gaccacggat catatctttt
    20641 tcacgaccgt cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac atttttctcg
    20701 atagcgtgca gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagt
    20761 gtcgcgatgg tttcagattt gatgacatca acctgcgcat aacgctcttg tttgtcggtg
    20821 atgcggtaag catcgctcag acgagcttca gccagtgcag caacgcgcgc gttcagcgct
    20881 tcgtttaccg gctccggctg ccagtcccaa cgcggtttac cggcttcttt caccagttca
    20941 ttgatgttct gaataacaac ctgctgttgt tcatgaccga agactactgc gcccagcatc
    21001 tggtcttcgc tcagcagttc agcttcagat tcaaccatca gtacagcggc ttcagtaccc
    21061 gcaacaacca gatccagttt gctctctttc aactcgtcct gagtcgggtt cagtacgtac
    21121 tggtcattga tgtaacctac acgggcagca ccaatcgggc cattgaacgg aataccagac
    21181 agagacaggg ctgcggaagc accaatcatc gcgacgatat ccgggttaac ctgcgggtta
    21241 acagaaacca cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg
    21301 cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg gcggccttca
    21361 cgacggaaga agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg
    21421 gtcagcggga agaagtcctg acctggtttg gcttttttct gaccaacaac ggtaacgaat
    21481 accgcagtgt catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg
    21541 gtttccagag tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat
    21601 gtaatatcct ttctctttct tagacagtac cttacggcac tggtgttaat acccgatctt
    21661 ctgcgcatcc tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca
    21721 ttagccgcgc gaacctctgc agcggaagat cattcatagc aacaatacat tagtttccag
    21781 tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc
    21841 aagaattagc gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta
    21901 cgtttcaggt agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg
    21961 ctgtggtgat cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc
    22021 agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa
    22081 acgattttag ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat
    22141 gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa
    22201 tttacgcgac gttaagcggc agtattctac tcgtagcgac ttgttatcgc aagacggtta
    22261 acattacgcc gggtattcaa ccaccaggcg acgaggcgca acgcggcctt catcgtcaat
    22321 ttcgcccata ccgataaact tgccgttctc accctctgta acgcgaacca gtccttccag
    22381 cggcgcaccg gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg
    22441 aagattcacc actggatagt ccgaagctgg actgtccatt ggcatcagta atggatcaag
    22501 taactccgca gccggaatat cctgctgttc ggcttgctca accagttcac gcagatgttc
    22561 cagggtcacc atccgttcaa ccggatattt acttaccgcc agacgacgca agtaaataac
    22621 atgcgcgcca caaccgagtt tttcgccgag atcgtcaatg atagtgcgga tataagtgcc
    22681 ttttgagcag tgaatttcca gctccagctc attgccttca tggcgaataa acagcaattc
    22741 ataaacggta atcgggcgcg cttcacgcgg aacttcaatg ccctgacgcg cgtattcgta
    22801 cagttttttg ccctgatatt tcagtgctga atacatcgaa gggatctgtt cgatatcgcc
    22861 acggaaggta tccagcgccg ccgccagttg ctctgcacta aaggttaccg gacgttcttc
    22921 aacgatttgt ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg
    22981 ataacgtttg tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat
    23041 tggcaacatg ccggtcgcca gcgggtccag cgcaccggta tgcccggcac ggttggcgtt
    23101 ataaatccgt ttcacttttt gcagcgcatc gttgctggac atcccctgcg gtttatccag
    23161 caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg
    23221 ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt
    23281 gacatgcgca tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata
    23341 cgcaggcgca tcgctttccc cagcaggctg cggatgaaac cagaagcttc ctgcagcgct
    23401 ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga ggaacgttac atatactttg
    23461 gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccagacga
    23521 ggatctttaa tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg
    23581 cgctgcgggc gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt
    23641 agcccctttt taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc
    23701 gccagcgtcg catcaggcaa tccatgccgg atgcggcgta aacgccttat cccgcatgga
    23761 accctaaaaa ccttaagcga tggtacgttg gatttcgatg atttcgaata cttcgatcac
    23821 atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca taccgttacg
    23881 gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt cgtagataac
    23941 cacgttgtcg cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat
    24001 acaacctgcg atggcaccaa atttcggtga tttgaatacg tcacgaactt ccgccagacc
    24061 gataatctgt tgtttcagtt ccggagacag cataccgctc attgccgctt tcacttcgtc
    24121 gatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt caatcacttt
    24181 acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcattgg acgccgcagc
    24241 cagggtagcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac
    24301 ttcgtcagta gacagtttca gcaaggagtc ggagatagct tccacagaac cctgtacgtc
    24361 tgccttcagg acgatattca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt
    24421 ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata
    24481 gagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc
    24541 cggaacgccg gacaggccga ggatttccac cggaatggac ggacccgctt ccagcacttc
    24601 ctgacccagt tcgttgcgca tcgcacgaac acgaccgtat tcgaagccac acagaacgat
    24661 atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac cacgaccttt
    24721 atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagttc
    24781 cagaacttcc gcctgcagca ggatagcgtc cagcagctca tcgataccag tacccgcttt
    24841 cgcagacaca tgtacgaact ggctttcacc gccccactct tccggcagga tgccgtactg
    24901 ggagagttcg ttcttaacgc gatccggatc ggcttctggt ttatcgatct tgttcactgc
    24961 aaccaccacc ggaacctgcg ccgctttcgc gtgctggatt gcttcgatgg tctgcggcat
    25021 tacgccgtcg tcggcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgagc
    25081 acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc
    25141 agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggccac
    25201 tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat
    25261 ggtcacaacc ggcgcgcgcg gttcagccgc tgcacccgtg tcacggtcgc tcattaccgc
    25321 ttcttccaac tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag
    25381 ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat
    25441 cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat
    25501 ggtttcgccg atcacaacgt cacggttaac ggcctgagca ggcttctgga agccttgctg
    25561 cagcgaagaa cctttacgtt ttccgccctt accgccacgt actgctgcgc gtgcttcttc
    25621 acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgcag ctttcgcgtt
    25681 acgaccacgg ccacggccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc
    25741 atgttgagaa gtagtaacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt
    25801 ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc
    25861 gagtttacga cgcgcttctt cttcggcttt acgcttgagc tctgcagctt cctgctcacg
    25921 gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc
    25981 tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt gttctgcggc
    26041 ctcacgttca gctttttgtt gcgcctcacg tttggccgat tcttctgcct cacgacgggc
    26101 ttgctcttcc gcttcacgct gcgcttgctc ttccgcagca aggcgttcag cctcttgcgg
    26161 atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt tgctttttcc
    26221 accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca atttgtccgg
    26281 gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg cagacacaga
    26341 gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc gttccacgga
    26401 ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt tccttcctgc
    26461 tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca taatcagtgc
    26521 tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc cctgttcggc
    26581 gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat cacgatctat
    26641 cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt cttcctgggc
    26701 ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa cggtcggctc
    26761 atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt cttccagcgt
    26821 cgagaagcct tcttctacca gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt
    26881 gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt cgtcaacggt
    26941 catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct gaccattacg
    27001 gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt gtttatcttc
    27061 atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacga actgcgccgg
    27121 gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag aaaccgcctg
    27181 aacacgcgcg ccacgcatac ctacgcaagc acctaccgga tcgatacgtt tatcgttggt
    27241 tttcaccgcg attttcgcac gagaacccgg atcgcgagcc gctgctttaa tttcaatcac
    27301 ttcttcgccg atttccggca cttcaatgcg gaacagttcg atcagcattt ccggcttgga
    27361 acgggtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataga gcacgccacg
    27421 aacgcggtcg ccaggacgga agttttcacg cggcagcata tcttcgcgca ggatcacggc
    27481 ttcagcgttg ttgcccagat ccagagagat gttgtcgcgg tttacttttt tcaccacgcc
    27541 ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac gttcggcttc
    27601 acgcactttc tgcacgataa cctgttttgc cgtctgggtg gtgatacggt caaaggtaac
    27661 agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt cataacgtgc
    27721 ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta accagcgacg
    27781 gaaggtgtca aaatcaccgc ttttgcgatc gatctgtaca cggacgtcga tctcttgctc
    27841 atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg
    27901 aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt tgttcatcgc
    27961 gggcttttca cctcatccag actattaaaa gtggggaacc aggttcgcct tctggatatt
    28021 actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt caccgtctac
    28081 cgctttgata acgccctgcc atttacgacg gttttgtacc gccatgcgga gaaccagagt
    28141 cacctcttct ccgacgaaac gggcgtagtg ttcagccgtg aacagtgggc gatcgagacc
    28201 cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat ccagcacagc
    28261 acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt cactatcaat
    28321 atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca gttcaaaacc
    28381 cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg tggacaagcc
    28441 cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca gataacaaaa
    28501 aaccccgata aatcggggct ttatataact gaaccctata accgcaactg cggtctggag
    28561 cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc acagtatatt
    28621 tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga cgaccttcgg
    28681 gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt ggcaaattct
    28741 actcgttttg ggtaaaaaat gcaaatactg ctgggatttg gtgtaccgag acgggacgta
    28801 aaatctgcag gcattatagt gatccacgcc acattttgtc aacgtttatt gctaatcacg
    28861 tgaatgaata tccagttcac tttcatttgt tgaatacttt tgccttctcc tgctctccct
    28921 taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata aaagatgatt
    28981 aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc aggctcctca
    29041 tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct cccggtaggt
    29101 caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact gctgtggatg
    29161 cgacaaaagg gagctgttcc ttatgcatat actgcaaatc tgggccagcc agacgaagag
    29221 gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc acgtctgatc
    29281 gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg cgcatttcat
    29341 aacaccactg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc cgtgactggc
    29401 accatgctgg ttgctgctat gaaagaagat ggcgtgaata tctggggtga cggtagcacc
    29461 tataaaggaa acgatatcga acgtttctat cgttacggtc tgctgaccaa tgctgaactg
    29521 cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg tcgtcatgaa
    29581 atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga aaaagcctac
    29641 tccacggact ccaacatgct cggcgcaacg catgaagcga aggatctgga atacctcaac
    29701 tccagcgtca aaatcgtcaa cccgattatg ggcgtgaagt tctgggatga gagcgtgaag
    29761 atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc gctgaacggt
    29821 aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg cggtcgtcac
    29881 ggcctgggca tgagcgacca gattgaaaac cgtatcatcg aagcaaaaag ccgcggcatt
    29941 tatgaagccc ctgggatggc actgctgcac attgcgtatg aacgtttgtt gaccggtatt
    30001 cacaacgaag acaccattga gcagtatcac gcgcatggtc gtcagttggg ccgtctgctg
    30061 taccagggac gttggtttga ctcccaggcg ctgatgctgc gggactctct gcaacgctgg
    30121 gttgccagcc agatcactgg tgaagttact ctggagctgc gccgcgggaa cgattattca
    30181 atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac gatggaaaaa
    30241 ggcgactcgg tgttctcgcc agatgatcgt atcggtcaat tgaccatgcg taacctggat
    30301 atcaccgata cccgcgagaa acttttcggt tatgccaaaa ctggcctcct ctcctcctct
    30361 gccgcttcag gcgtgccgca ggtggagaat ctggaaaata aaggccagta attcgacatc
    30421 aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc tttctgagca
    30481 tcacataatc gaaaacatca tggtttgcat ccacaacccg tgagtctcct ttatagtgtg
    30541 caatcacttc ctccagcgtt ttcggttgtt ccggctttgt tacccccatc cacgcattaa
    30601 tcaggtaatt attgtaagct gtggaaaaga tgttgttttc cgttgtgcga tccacgccat
    30661 ccccaagaac ggggctatac cagataaaca tcgggacatg atatgcctgc tggctggcct
    30721 ccctgcctcc atgaaaatag acgttcttct tcgtagggtc acgttccagg ccgtggtcgg
    30781 caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaacctga cctagcaaac
    30841 tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc ggttgaaaca
    30901 cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa
    30961 caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc ggcaacaaca
    31021 attcatcaaa tcctctgaca taaactgttt ccatggcgcg catggcgata ctggtaactg
    31081 ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt
    31141 gattagccat attaataatg ttgtccgggt aattatgaat gtcatgactc aaaacggaat
    31201 cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcaccgctt attgcttgat
    31261 taaacagttt gatctgtttt ctttgtgctt cgacttgcgg tgtcgtagag cgtgtatatc
    31321 catacaaaga catattgtcg acgcgtacag actcccccac aatcaacacg taggtatcaa
    31381 tacctgtatc cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc
    31441 tttgatgctc tttcgctgct aaagcaaaat aattgagatt gaaaaacggt gtataggtag
    31501 caaatcgcga cgctagtata tatggactga acgctttttt attttttgca tctttataag
    31561 caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca
    31621 cttttttcgt cggcaaggaa acatcatatt ttattattac tg
//
LOCUS       DAZGAP010000034        31098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGAP010000034 DAZGAP010000000
VERSION     DAZGAP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.034"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            143..685
                     /gene="zapC"
                     /locus_tag="ACHLAM_001334"
     CDS             143..685
                     /gene="zapC"
                     /locus_tag="ACHLAM_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO7852893.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFNLEQAV"
     gene            complement(682..1791)
                     /gene="ycbX"
                     /locus_tag="ACHLAM_001335"
     CDS             complement(682..1791)
                     /gene="ycbX"
                     /locus_tag="ACHLAM_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO7852894.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEVLATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            2035..4143
                     /gene="rlmKL"
                     /locus_tag="ACHLAM_001336"
     CDS             2035..4143
                     /gene="rlmKL"
                     /locus_tag="ACHLAM_001336"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO7852895.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4155..6062
                     /gene="uup"
                     /locus_tag="ACHLAM_001337"
     CDS             4155..6062
                     /gene="uup"
                     /locus_tag="ACHLAM_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7852896.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6192..7445
                     /gene="pqiA"
                     /locus_tag="ACHLAM_001338"
     CDS             6192..7445
                     /gene="pqiA"
                     /locus_tag="ACHLAM_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO7852897.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADDPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7450..9090
                     /gene="pqiB"
                     /locus_tag="ACHLAM_001339"
     CDS             7450..9090
                     /gene="pqiB"
                     /locus_tag="ACHLAM_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO7852898.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9087..9650
                     /gene="pqiC"
                     /locus_tag="ACHLAM_001340"
     CDS             9087..9650
                     /gene="pqiC"
                     /locus_tag="ACHLAM_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO7852899.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9906..10073
                     /gene="rmf"
                     /locus_tag="ACHLAM_001341"
     CDS             9906..10073
                     /gene="rmf"
                     /locus_tag="ACHLAM_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO7852900.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10143..10661)
                     /gene="fabA"
                     /locus_tag="ACHLAM_001342"
     CDS             complement(10143..10661)
                     /gene="fabA"
                     /locus_tag="ACHLAM_001342"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO7852901.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10730..12490)
                     /gene="ycbZ"
                     /locus_tag="ACHLAM_001343"
     CDS             complement(10730..12490)
                     /gene="ycbZ"
                     /locus_tag="ACHLAM_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7852902.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12676..13128
                     /gene="matP"
                     /locus_tag="ACHLAM_001344"
     CDS             12676..13128
                     /gene="matP"
                     /locus_tag="ACHLAM_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO7852903.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13204..14256)
                     /gene="ompA"
                     /locus_tag="ACHLAM_001345"
     CDS             complement(13204..14256)
                     /gene="ompA"
                     /locus_tag="ACHLAM_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO7852904.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(14612..15121)
                     /gene="sulA"
                     /locus_tag="ACHLAM_001346"
     CDS             complement(14612..15121)
                     /gene="sulA"
                     /locus_tag="ACHLAM_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO7852905.1"
                     /translation="MYTSGYAHRASSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15339..15968
                     /gene="sxy"
                     /locus_tag="ACHLAM_001347"
     CDS             15339..15968
                     /gene="sxy"
                     /locus_tag="ACHLAM_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO7852906.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15931..18093)
                     /gene="yccS"
                     /locus_tag="ACHLAM_001348"
     CDS             complement(15931..18093)
                     /gene="yccS"
                     /locus_tag="ACHLAM_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO7852907.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18103..18549)
                     /gene="yccF"
                     /locus_tag="ACHLAM_001349"
     CDS             complement(18103..18549)
                     /gene="yccF"
                     /locus_tag="ACHLAM_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO7852908.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18672..20726
                     /gene="helD"
                     /locus_tag="ACHLAM_001350"
     CDS             18672..20726
                     /gene="helD"
                     /locus_tag="ACHLAM_001350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO7852909.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWQRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20758..21216)
                     /gene="mgsA"
                     /locus_tag="ACHLAM_001351"
     CDS             complement(20758..21216)
                     /gene="mgsA"
                     /locus_tag="ACHLAM_001351"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO7852910.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21312..21974)
                     /gene="yccT"
                     /locus_tag="ACHLAM_001352"
     CDS             complement(21312..21974)
                     /gene="yccT"
                     /locus_tag="ACHLAM_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO7852911.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22147..22560
                     /gene="yccU"
                     /locus_tag="ACHLAM_001353"
     CDS             22147..22560
                     /gene="yccU"
                     /locus_tag="ACHLAM_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO7852912.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22605..22922)
                     /gene="hspQ"
                     /locus_tag="ACHLAM_001354"
     CDS             complement(22605..22922)
                     /gene="hspQ"
                     /locus_tag="ACHLAM_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO7852913.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22980..24170)
                     /gene="rlmI"
                     /locus_tag="ACHLAM_001355"
     CDS             complement(22980..24170)
                     /gene="rlmI"
                     /locus_tag="ACHLAM_001355"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO7852914.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24265..24543
                     /gene="yccX"
                     /locus_tag="ACHLAM_001356"
     CDS             24265..24543
                     /gene="yccX"
                     /locus_tag="ACHLAM_001356"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO7852915.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(24540..24869)
                     /gene="tusE"
                     /locus_tag="ACHLAM_001357"
     CDS             complement(24540..24869)
                     /gene="tusE"
                     /locus_tag="ACHLAM_001357"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO7852916.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24960..25619)
                     /gene="yccA"
                     /locus_tag="ACHLAM_001358"
     CDS             complement(24960..25619)
                     /gene="yccA"
                     /locus_tag="ACHLAM_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO7852917.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(26029..27048)
                     /locus_tag="ACHLAM_001359"
     CDS             complement(26029..27048)
                     /locus_tag="ACHLAM_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7852918.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(27026..27268)
                     /locus_tag="ACHLAM_001360"
     CDS             complement(27026..27268)
                     /locus_tag="ACHLAM_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO7852919.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27336..29774)
                     /locus_tag="ACHLAM_001361"
     CDS             complement(27336..29774)
                     /locus_tag="ACHLAM_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7852920.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRFGIKGGFYWSTEKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQEKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINDGLA
                     AECKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQLETEQND
                     QKAEQNSQNALQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATYQET
                     FDEKNQAEVQEDDPEEIEGAEHPNNENAGNDQHHTSDSETGEAADPLIAVNGHHEITS
                     TSRTCDHLMIDLETMGKNPNAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SAIWQKLIPSQADF"
     gene            complement(29867..30055)
                     /locus_tag="ACHLAM_001362"
     CDS             complement(29867..30055)
                     /locus_tag="ACHLAM_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7852921.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(30052..30240)
                     /gene="dicB"
                     /locus_tag="ACHLAM_001363"
     CDS             complement(30052..30240)
                     /gene="dicB"
                     /locus_tag="ACHLAM_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7852922.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(30726..30878)
                     /locus_tag="ACHLAM_001364"
     CDS             complement(30726..30878)
                     /locus_tag="ACHLAM_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7852923.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
BASE COUNT         7804 a         7547 c         7994 g         7753 t
ORIGIN      
        1 tcagggcttt attttaagcc ctggttgttt tatattcatc gctgttgctt atttagacat
       61 tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt tgctgcggtt
      121 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
      181 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
      241 ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt gcgttgacga
      301 cgccgcgctc tatttctcat ttgaagaaaa atgccgcgat tttaatttat ctaaagaaca
      361 aaaagccgaa ctggtgctta atgcgctggt agcaattcgt tatctcaaac cgcaaatgcc
      421 gaaaagctgg cattttgttt cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
      481 ctgcgtttgg ttgagcgaca ctcacgagca agtcaatttg ctggtcgttg aatctggcga
      541 aaatgccgca ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
      601 gctgggcgac gccatcaaaa tcatgaacga caggctgaaa ccgcaggtta atgttgacag
      661 cttcaacctc gaacaggcag tctaacgcgt cagcttaagc gcagtcttcg gtacacagct
      721 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg tgacttcgcc
      781 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
      841 aattccctga ttttctaatt gctccagcaa cacctgctgg ttatttccac gaaatgcctg
      901 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttga cggtatcatc
      961 agcggcggct gcgccgtaaa ttttagccgg agccgttgcc agaacttcca cctcatcgcc
     1021 aacgcgaatt acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     1081 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     1141 ccctttttct ggactgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     1201 caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg acgcgccgga
     1261 aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag
     1321 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagggg
     1381 tacagtgttg tggcgtttca cgcgccgcgt catttgtggc cccacccagc gcaactgcac
     1441 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggtgcaa ttcgcgcggt
     1501 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     1561 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     1621 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     1681 cataaagatg cgatcgaagg ccagaccact gacatctgcc agggcatgtg taagaccaat
     1741 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     1801 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     1861 acaattttct tagcgcctgt cccattaggc tattttattt gtcagtttgg cccagggaag
     1921 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctgtc catgtccaga
     1981 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
     2041 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaac
     2101 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
     2161 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
     2221 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
     2281 gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
     2341 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
     2401 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
     2461 ctgcataaag aaaccgccag tattgccctt gatctcagtg gtgatggttt acatctgcgt
     2521 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgcgattgtt
     2581 atgcgatccg gctggcagcc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
     2641 ttgttgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca ccgtggacgt
     2701 tggggcttta gcggctgggc acagcatgat gaagccatct ggcaggaagt gaaagcagaa
     2761 gcgcaaaccc gcgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
     2821 agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa
     2881 ctgatcacct ttgaagtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
     2941 tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga accggcgctg
     3001 attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggcgg ctggaatctc
     3061 tctttattca gtgcctcgcc ggatctgtta agttgcctgc agttgcgtgc agacaaacag
     3121 tacaaagcga aaaacggtcc attagactgc gtgcagaaaa attaccacgt tgccgaaagc
     3181 acaccagaca gcaaaccggt aatggcagcg gaagactacg ccaaccgtct gcgtaagaac
     3241 ctcaaaaaat tcgagaagtg ggcgcgtcag gaaggcattg aatgctaccg cctgtacgac
     3301 gccgatctac cggaatacaa cgttgcagtt gaccgctacg ccgattgggt ggtggtgcag
     3361 gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct gttcgatatt
     3421 atcgctgcaa ccatttcggt attggggatt gcgccaaaca aactggtgct gaaaacccgt
     3481 gaacgccaga agggcaaaaa tcagtaccag aaactgggcg agaagggcga gtttctcgaa
     3541 gttaccgaat ataacgctca cttgtgggtg aacctgacgg attacctcga taccggtctg
     3601 ttcctcgatc accgtatcgc ccgtcgtatg ctcggtcaga tgagcaaagg caaagatttc
     3661 ctcaacctgt tctcttatac cggcagcgcc accgtgcacg cgggattagg cggtgcacgc
     3721 agcactacca ccgtggatat gtcgcgtact tatctggagt gggcagaacg caacctgcgt
     3781 ctgaatggcc taaccgggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
     3841 cgtgaggcaa atgaacagtt cgatctgatt tttatcgatc cgccaacctt ctctaactca
     3901 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat gaaagatttg
     3961 aaacgtctgc tgcgtgctgg tgggacgatc atgttctcga acaacaaacg tggcttccgt
     4021 atggatcttg acggactggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
     4081 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
     4141 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
     4201 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
     4261 gccgcaacgg cgcaggcaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
     4321 atgacggtcg cattatttac gagcaagatt tgattgtagc tcgtctgcaa caggatccgc
     4381 cgcgtaacgt tgaggggagt gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
     4441 aatacctgaa acgctatcac gatatttcgc gcctggtgat gaacgacccg agcgagaaaa
     4501 atctcaacga actggcgaag gttcaggaac agttggatca ccacaacctg tggcagctgg
     4561 aaaaccgcat taacgaagtg ctggcgcaac tggggttaga tcctaacgtt gcgctgtcgt
     4621 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agcaatccgc
     4681 gcgtattgct gctcgacgaa ccgactaacc acctggatat cgaaaccatc gactggctgg
     4741 aaggattcct taaaaccttc aatgggacga taattttcat ctcccacgac cgttcgttta
     4801 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagcttgtg acctatcctg
     4861 ggaattacga ccagtacctg ctggaaaaag aagaagccct gcgcgtggaa gaactacaaa
     4921 atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg gatccgccag gggatcaaag
     4981 cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcaatgcgt cgtgagcgtg
     5041 gcgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
     5101 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt tgacggtaag caactggtga
     5161 aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatggat
     5221 gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaggcggac agcgggcgta
     5281 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgtgcg gaactggatc
     5341 ccgataaaac ggtgatggat aaccttgctg aaggtaagca agaggtgatg gttaacggca
     5401 agccacgtca tgttttgggc tatttgcagg acttcctgtt ccatccgaaa cgggcgatga
     5461 cgccggtacg tgcgctttct ggcggtgagc gtaaccgctt gctgttggcg cgtttgttcc
     5521 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gttgaaacgc
     5581 tggaactgct ggaagaactg atcgacagct accagggcac ggtattgctg gtgagccacg
     5641 atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc ggcggtaaaa
     5701 ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca gcaagaacag tatgtggcgc
     5761 tcaaacagcc tgcggtgaaa aaaatcgaag aagccgccgc gccaaaagca gaaactgtaa
     5821 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
     5881 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaggtggcg gatgcttcct
     5941 tcttcagtca gccgcatgag cagacgcaaa aagtgctcgc cgatatggct gctgcagagc
     6001 aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
     6061 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg cctgatgaac
     6121 tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata gcgcatcatt
     6181 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
     6241 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
     6301 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc tatgcgttgg
     6361 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcgg
     6421 gagtcaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
     6481 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
     6541 ccattctgtt actggtgaat cgcgcagaat taccggtccg tttaaaagag caactggcac
     6601 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg ggtgtgctgg
     6661 tcagtttcgt taaactgatg gcttacggca gcattggcgt aggcagcagc tttctcccct
     6721 ggtgtttatt ttgtgtcctg caactgcgtg cttttcagtg cgttgatcgt cgctggttat
     6781 gggacgacat cgccccgatg ccagaactgc gccagccgct aaaacctggc gtcacgggga
     6841 tacgccaggg gttacgttcg tgctcctgtt gtacagcaat tcttccagct gatgatccgg
     6901 tgtgtccgcg ctgtggtact aaagggtacg ttcgtcgtag aaacagcctg caatggacac
     6961 tcgcgctgct tgttacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
     7021 tgacggattt attaggctcg aagatgccat cgacgattct cgctggggtc atcctgttat
     7081 ggagcgaagg atcttatccc gtcgctgcgg ttatctttct ggccagtatt atggtgccaa
     7141 cgttaaagat gatcgccata gcgtggttgt gttgggatgc caaagggcat ggcaagcgcg
     7201 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
     7261 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
     7321 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
     7381 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
     7441 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
     7501 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
     7561 tacagccatc aggggccgga agtgaccctg atcaccgcga atgcggaagg aatagaaggt
     7621 ggtaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg
     7681 gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat ggaaaaattg
     7741 ctgcataaag ataccgtctt ttgggtggtg aaaccgcaga ttggtcgaga agggattagc
     7801 ggcctgggaa cgctgctgtc tggtgtttat atcgaactgc agccaggcgc gaaaggcagc
     7861 aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga tgcgaaaggt
     7921 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tcccgtgctg
     7981 ttccgtggct atcgggtagg ttcggttgaa accagcacct ttgatacgca aaaacgcaat
     8041 atcagctatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
     8101 ttctggaaag atagtggcat tgcggttgat ttgacgtcag cgggaatgcg tgtggagatg
     8161 ggttcattga caacgctgct tagtggcggt gtcagctttg atgtgccgga aggtctggat
     8221 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
     8281 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
     8341 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
     8401 gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg tattccggta
     8461 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
     8521 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
     8581 gtcactggcg cactgtatgt tgatctcgat ttctatccaa atacgcctgc aataaccggt
     8641 attcgtgaat ttaatggtta tcagattatc ccgaccgtta gcggcggtct ggcgcaaatc
     8701 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
     8761 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
     8821 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagctgcc gacggatatg
     8881 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
     8941 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
     9001 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
     9061 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcggcactgt
     9121 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
     9181 agagcggtac acaaagcacc gccagccagg gtaatcgcct gttatgggtt gagcaggtcg
     9241 ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
     9301 tgattgccaa caacaacctg tgggccagcc cgttggatca acagttgcgc aacactctgg
     9361 ttgccaacct gagcacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
     9421 cccaggatac gctcaatgtt accgtaacgg agtttaacgg gcgatatgat ggcaaggtca
     9481 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
     9541 tggaaggggt gcaaactcag gatggttacg atgaaatggt taaagtgctg gccagtgtct
     9601 ggagtcagga agccgcttct attgcgcaag agataaagcg tctaccgtaa tcataaagat
     9661 ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt ctaaggtgcg
     9721 gaaaaagttg ttcctcttca cattttttgt acaactgaca tgcccgtgta gctcacaaat
     9781 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
     9841 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
     9901 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
     9961 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat caaaggtcac
    10021 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    10081 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgattat
    10141 aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    10201 agacgaccat caaccagcac ttcgccatct gccagaccca taatcagacg tcgattaaca
    10261 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccggtg
    10321 aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag ccagccgagg
    10381 tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat aaccggatcg
    10441 ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    10501 taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc catcatcagc
    10561 atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgcg accagaggca
    10621 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    10681 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    10741 taaaccagtt cagccaacgc aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    10801 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    10861 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    10921 tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga atgttagcag
    10981 tggggataat gacaccttgt ttcccggtta actcacgttg ctggcaaata gcaaagaagc
    11041 cttcgatttt ctcatttaaa ccaccgaccg gctgggcacg accgaactga tcgactgaac
    11101 ctgtgatagc gatactctga ttcaccggca catcggcgag ggcgcttatc agggcgcaga
    11161 gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca aatgtcagcg
    11221 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcctgcatga
    11281 tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    11341 actcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    11401 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    11461 tttcaatcag gatttgctcc tgaagaatct catcctgcat gcgttcagcg aggaaacctt
    11521 cacgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag gtgtcgccat
    11581 cgcacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta agcggaagtg
    11641 tttcttgttc accggtgtag cgggctgctt cgcggataag taccggccag gcatccgctc
    11701 ccggtgcagg taagtgatta tgtcttgcgg tcaatgttac ccagcgacac cactggctta
    11761 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    11821 aaagctctgg ttccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    11881 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    11941 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    12001 gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga tgcaccagac
    12061 ctggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgttccgctt
    12121 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    12181 cgttacgtaa gcggatggtg tgcccggaaa cctcatagtg accgcccacc agttggcctg
    12241 catcgctttg tagcgcacgc gcggcatcgg caataagatt cagatactca gactcttccg
    12301 gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    12361 attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    12421 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    12481 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    12541 cactacagca cacgtagaaa gcaccgacaa tactcctggc atgggcgtta aagctcacag
    12601 gatggagatt cttttcttca ctggcccaaa aagctgatat tctgtaaaga gttacacggt
    12661 aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg ttggaaatgg
    12721 aagtatctgg tgaagaagca ccgcgaaggg gagttaatca cccgttacat agaagccagt
    12781 gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa atgaacccgt gctggtaaat
    12841 ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca aaccattcgg
    12901 gccagacgga agcgccattt taatgcggaa catcagcata cgcgcaaaaa atcgatcgat
    12961 ctggaattta ttgtctggca acgtctggct ggtcttgcgc agcgtcgcgg taaaacgctt
    13021 tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata cgcgaataaa
    13081 atgtcttctt tgaagcagga tctgcaggca ttgctgggta aggaataact gacgaaagtc
    13141 agttcaattt tctaaagacg aaaaaaaacc ccgcagcagc ggggtttttt accagacgag
    13201 aacttaagcc tgcggctgag ttacaacgtc tttgatgcct ttaacttcga tctctacgcg
    13261 acgatccgga gccaggcaat cgatcagtgc agcacgctgt ttcacgttgt cacaggtgtt
    13321 gccagtaacc gggttggatt cgcccatacc acgtgcggag attttgtctg ccgggatacc
    13381 tttagagatc aggtaatcaa caacggactg agcacgacgc tcggacagag cctggttata
    13441 agcgtcagaa ccgatgcggt cagtgtaacc cagaacaact acggaaccgt ctttcggatc
    13501 caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg gtttcagggt
    13561 tgctttgttg aaggtgaaca gaacgtcaga cttcagagtg aagtgcttgg tctgtacttc
    13621 cggtgccgga gctggagccg gagcaactac tggagctgct tcgccctgac cgaaacggta
    13681 ggaaacaccc aggctcagca tgccgttgtc cggacgagtg ccgatggtgt gtgcgtcacc
    13741 gatgttgttg gtccactggt attccagacg ggtagcgatt tcaggagtga tcgcatactc
    13801 aacaccgcca gcgaagaccg gagaaacgcc ggtgtcgtgg tctttagtgg atgcgccacc
    13861 aggtacgtta gacttggtgt ctgcacgcca taccatacca cccagacgag tgtaaacgtc
    13921 cagatcgtca gtgattgggt aacccagttt agcggtcagc tgaacgccct gagctttgta
    13981 tgcgccgttg atgttgtcgc ctttgtacgg catacgacct aaccagtcgt aacccatttc
    14041 aaagccaaca tacgggttaa cctggtaacc accaaaagca cctgcaccca gttggttttc
    14101 gtgggtcgga ccattgttag gaataaaacc agtgtcatgg tactgggacc agcccagttt
    14161 agcaccagtg taccaggtgt tatctttcgg agcggcctgc gctacggtag cgaaaccagc
    14221 cagtgccact gcaatcgcga tagctgtctt tttcattttt tgcgcctcgt tatcatccaa
    14281 aatacgccat gaatatctcc aacgagataa cacggttaaa tccttcaccg gggatctgct
    14341 caatattaac tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct
    14401 acaacgtagt tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg
    14461 tataaggtac gtttaatctt ttttgtcagc gacaatttac agaagaaaat cgcggaaacc
    14521 gcttcagaca agcctccgca aggaaaatta gtcacgactg aaagcattgg ctgggcgaca
    14581 aaaaaagttc caggattaat cctaaattta cttaatgata caaattagag tgaattttta
    14641 gcccggaaag ttgtctcgtt gcgtgagagg atgcgcttac cggacgcata ataaacccca
    14701 tagcgttacc ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat
    14761 ctgccaacca accgatcacc acgctgtaat tgcccgtgcg taaagcgcga accattgact
    14821 caacggtgtg gcaaggggag agctggctaa tctgcattac tttcgttaag ggtagcccag
    14881 atgcctgaac ccattcgcga ctcagttttt gttgcggtgt taaccagagt tgccagcgcg
    14941 attgctgacc gagttgctgt aacaatggca acagtagaag ttgcgtcatc atgggctgat
    15001 cttcgcgata gacaacttca ctgataagcc cggctgtagt gttttccgta gagacacgcg
    15061 caattttact tgctgcggat gagaacgacg aagcacgatg tgcatagcct gaagtgtaca
    15121 taatcaatcc agccctgtgg gttactgtat gaatatacag tacatccggt gacgacaaag
    15181 atcaacccta ttttcggaaa gagcctcgca aattttgtcg ttagtgacgg gaaaacataa
    15241 attaatcttg ccccttaaga ataagttgcc tattttcgta gttaacggat ccgttaatgt
    15301 gaatcattct tttatgttat gattttaaaa ggaattttat gaaaagcctc tcctataagc
    15361 ggatctataa atcacaagaa tacctggcaa cgttgggcac aattgaatac cgatcattgt
    15421 ttggcagtta cagcctgacc gttgacgaca cggtgtttgc gatggtttct gatggtgagt
    15481 tgtatcttcg ggcttgtgag caaagtgcac agtactgtgt aaaacatccg cctgtctggc
    15541 tgacatataa aaagtgtggc cgatccgtta ccctcaatta ctatcgggtt gatgaaagtc
    15601 tatggcgaaa tcaactgaag ctggtgcgtc tgtcgaaata ttctctcgat gctgcgctga
    15661 aagagaaaag cacgcgcaat acccgggaaa gactgaaaga tttgcccaat atgtcttttc
    15721 atctggaagc gattctcggg gaggtgggga ttaaggatgt acgggcgtta cgtatacttg
    15781 gggcaaaaat gtgttggttg cgactgcggc agcaaaacag tctggtgaca gaaaagattc
    15841 tgtttatgct tgaaggggcc attatcggca ttcatgaagc tgcgctcccg gtggcacgcc
    15901 gccaggagct tgcagaatgg gctgactctc ttacgccgaa acaggagttt cctgcggaac
    15961 ttgagtaatc tggcgttgca gacgaccaat ctctggcagt aatgcaatca ataacccaac
    16021 ttgttgtacg accagaggtt ctttgctgtc tgcccgtggt tcaagttgct gcatccgctg
    16081 tttcaggcca gctaatgcct gattgacgcg ttcttcatca gcaggttgat gatgtaacgc
    16141 gtcatcaaca tagcaaactg cgtcatcaag aaacgccaga atttcaggat tagttaactg
    16201 ctcccggtga gcaccgaggg ctgagatata gctggtaaac gtatggttaa ggcacagcaa
    16261 ccgaaacgcg gcttcgcgaa tttgcggggt aacgttcggt tcgctggaca tatttgatac
    16321 caccgacgcc agttcagcat cacggttgtg tgcatcgcgg cgggcaatac gatacgccag
    16381 acggttatca cgcccctgat ggtattgctc cagtatggca tcgagatacc ggcagttggc
    16441 ctcggtggcg cgttcgagca ttcgcggcag attgcgaaac ttccagtcag gccagatgta
    16501 gctcactgcc gcccacgcaa tggcacaacc aatcagcgta tcgattacgc gaggtaacgc
    16561 cacttcaaaa ccttcaccca gcaagttgaa acacagtagc accaaaagtg tgatgaacat
    16621 cgttgcatgg gcgtattgca cgttacggaa ggcaaaaaag agcacgccgg taataaccag
    16681 cagcaccagc tgcccttcca gtgatggcac aaaccacagc acaggaatac caatcgcgat
    16741 acctaccagc gtaccaataa tccttaactt caggcggtgg cgcgtggcgt tatagtttgg
    16801 ctggcagaca aacaaacttg tcagcaagat ccaatacccg tgatgcattc cggttatctg
    16861 aatgatggcg tagccgaagc acaacaccag cgacattctt accgcatgac ggaagagggc
    16921 ggattccggc gtgaagtgac ggctaagacg cagccagata tcactcaatc cgtgcgggct
    16981 gtcatcagcg agctcatttt cgtcattatt atggggcagt gcctgggcct gttctgattc
    17041 aattgttgcc agttgggcat caatggcacg taaattgttc agtaaaaatc ctagtgtttt
    17101 gagtaaatca gcaggagcgc cgttatcgcg catccgctcc agcgcagcat caatatgcgt
    17161 aaaagcgcgc tcaaaatgcg gatcatgttg ataaggctga cgcagcaaaa tacagcgtga
    17221 cagttgctgg cacgcctggc cctgcatcga catcagccgc tgaaaacgga acagcacgtc
    17281 gctgtggcga aaatgttcac gcaatgtttg atattgaata tgagaagagc tggcacgctc
    17341 gtgaatatcc tgcgcgacaa agtaataatg cagtgtgcga cgcgttcctc gttgaccacg
    17401 atcgccacgt aagcgggtca gcagcgagag tttcgtctga ttcaatgtcg ccatcagctg
    17461 accgttggcg agagccaaat cgtacagcgg tgcctggctt tcatcttcaa tatcaggatc
    17521 aaacatgcgc gacttgagct caagataacg cgccagttgt tcatagcaac gcgccaggtt
    17581 gtcctgcagc gggcggaccg ggaacagcag atgaccaata agtgtcagga cgttgtacca
    17641 gacggcaccg gccagcagat acatcggctg ctgataccag tgctcataca gtgacgtccc
    17701 caacatagtg taaatggcga tcagtaacgc accgaaggca attgttgcat agcgttgacc
    17761 cagaccgccg agcaaaatga agccgctggt agagagcgtt aaaccaattg caaatagcca
    17821 gggccaggga aacagcaatt ctactgaggc cgaggcgata aaaaagcaga acagcgtaat
    17881 gatgaggtta cgcaaacgtc ccgccagtcg gtcatcgaga tcggtcagcg ccgctgccac
    17941 catccccagc gttagcggaa tcgtcagttt tacatcaccc agccaccacg gaaacgctgt
    18001 ggttccacaa agcgcaataa aaatacgcgc gtaatacagc caggcgctgt tccaggtata
    18061 gcgtttgagc aaaggactta gcataaaggc catattagtg gattattcaa aacgacgacg
    18121 tgcattggct tcacgcgcag cttgcgctgt ttctaccgat accacgcgac gaccgaccgg
    18181 ccatagtgca atagcggcaa ttttaaagtt cgcaatgccg acaggaatgc caatgatgga
    18241 aatacattgt gcgatgcccg ttgcaatgtg catcaggcat aaccaccagc caaagaaaat
    18301 cagccagaaa atattcaata ccgtaccgcc agtatttagc agcacatttt tgccagccgg
    18361 gttcagttca tcgacatgaa tagcttcatt gccataaggc accagagaca gtttagtgat
    18421 ctcccagcag gatcgtgtca gcggtaaggt aaaaatcagc acaatactga ccagagtcgc
    18481 caacagccag cccagagtgg tggcaaatcc gccaagcaca aagttcagaa tgttcaaaac
    18541 ggtacgcata aaacctcgct ttactgtggt tttcagtaat gggcggcaat tgtaacgttt
    18601 ttttgggctg gagcacgttt tctctgacgg ttacactgat aagaaataat ttcgtgtgga
    18661 tctacagagt catggaactg aaagcgacaa cgcttggaaa acgtctggca cagcaccctt
    18721 acgatcgggc ggtgatcctc aatgccggga ttaaagtctc cggcgatcgc cacgaatacc
    18781 ttattccttt caatcaatta ctggcgattc actgtaagcg cggtctggta tggggcgagc
    18841 tggaatttgt actgccgggc gaaaaagtgg tgcgtctgca cggtaccgaa tggggcgaga
    18901 cgcagcgttt ttaccatcat cttgatgctc actggcagcg gtggagtagc gagatgagcg
    18961 aaattgcatc tggtgtttta cgccagcaac tggatttgat tgccacgcgt actggagaaa
    19021 ataaatggct gacgcgtgag caaacctctg gcgttcagca acaaatccgc caggctttgt
    19081 cggcgttgcc gttgccggtt aaccgactgg aagaattcga taactgccgt gaggcatggc
    19141 gtaaatgtca ggcctggttg aaagatattg aaagcgctcg gttgcagcat aaccaggcgt
    19201 ataccgaagc catgcttacc gagtatgcgg attttttccg ccaggtcgag tcttcaccgc
    19261 tgaatccggc gcaggcccgg gcagtcgtta atggcgagca ttctctgtta gtgctggctg
    19321 gtgcaggaag cggaaaaacg tcggtgctgg tggcccgtgc aggctggttg ctggcgcgtg
    19381 gtgaagcgtc ccctgagcaa attttattgc tggcgtttgg tcgcaaagcc gctgaagaga
    19441 tggacgagcg tattcgagaa cgactacata ccgaagacat taccgcacgc acatttcatg
    19501 cgctggcgct gcatattatt cagcagggca gcaaaaaagt tccgatagtc agcaaactgg
    19561 aaaatgatac cgctgcccgt catgaacttt ttattgctga gtggcgcaag caatgcagcg
    19621 aaaagaaagc gcaggcgaag ggctggcggc aatggctgac ggaagaaatg cagtggtcag
    19681 tgccagaagg taatttctgg gatgatgaaa aattacagcg tcgtcttgcc tctcgcctcg
    19741 atcgctgggt aagtctgatg cggatgcacg gtggtgcaca ggcagaaatg attgccagtg
    19801 cacccgaagt gattcgcgat ctgttcagta aacgtatcaa gttgatggcc ccgttattaa
    19861 aagcctggaa aggtgcgctg aaggcagaaa acgctgtcga tttttcgggc cttattcacc
    19921 aggcgattgt gattctggag aaaggtcgct ttatcagccc gtggaagcat attctggttg
    19981 atgaatttca ggatatctcg ccgcagcgcg cagcgttgtt agcggcatta cgcaagcaaa
    20041 acagtcagac gacgttgttc gccgttggtg atgactggca ggcgatttat cgcttcagcg
    20101 gtgcgcaaat gtcgctcacc accgctttcc atgaaaactt tggtgaaggt gatcgctgcg
    20161 atttagacac gacttaccgt tttaacagtc gtatcggtga ggtggcaaac cggtttattc
    20221 agcagaaccc aggccagctg aaaaagccgc taaacagctt aaccaatgga gacaaaaaag
    20281 ccgtcacgtt attggatgag agccatcttg acgctttgct ggataagctc tctggttatg
    20341 ccaaaccgga agagcgcatt ctgatcctgg cgcgttacca tcacatgagg cctgccagcc
    20401 tggaaaaagc ggcaacacgc tggccgaagt tgcaaatcga ctttatgacc attcatgcca
    20461 gcaaagggca acaggcggat tacgtcatca tcgttggctt gcaggagggg agtgatggtt
    20521 ttccggctgc ggcgcgggag tcgattatgg aagaggcgct actgccatcg gttgaggatt
    20581 tcccggacgc tgaagaacgg cggttaatgt acgtggcgct gacccgggca cgccatcggg
    20641 tatgggcact gtttaacaaa gagaatccct ctccatttgt cgaaatactg aaaaatcttg
    20701 atgtgccggt ggcgagaaaa ccgtaagaaa caggtggcgt ttgccacctg tgcaatatta
    20761 cttcagacgg tccgcgagat aacgctgata atcggggatc agaatatcga ccgcgtcgtt
    20821 gaaatgcggc gactggataa tgaagtctgc cgttgccacg ttggtggcga ccggaatgtt
    20881 ccataccgtc gccagacgca gcaaggcttt cacgtcaggg tcgtgcggca cggcgtttag
    20941 tgggtcccag aagaaaatca atacatcaat tttcccttct gagatcaatg cgccaacctg
    21001 ctggtcaccc cccattgggc cactcaacat cgcgttgacg ttcatgccgg ttgcacggga
    21061 aattaagtta ccggtagtgc ctgttgcata cagtacgtgt tgttccagta acggttgatg
    21121 ccgttccacc cagctcatca gcatttgttt gcagtgatcg tgtgccacca gcgcaatatg
    21181 tttccgcgca ggtaaagtgc gtgtcgtcag ttccataatg tacatccgta gttaactttc
    21241 ctacagatta ctgtaagcac ttatcgctgc aagataaaga ccgaaaaagc ctgcgcacag
    21301 gcacaaaaat ctcaggaaga tggttgtttt tccgcccatt gcaggaaagt atttcgcgtt
    21361 tgtgggtcag ccagtttaaa ccaatacttc agccgttgtt ctgtgagcac ctgagactgc
    21421 ggtggaatag catccagctc ggaaacaccc gaaagcagcg tactatcatc tgccatcatc
    21481 gtggcaaatt gcggtagtga agcgcgtttc gcggatttgt tatagcgttc aacctcgacc
    21541 tcataatcaa tcgtttttgc cgttgaggta atggcgagaa tatccagctt taccggaatc
    21601 ggcgtcgcat cgccatccaa caattcaagg cgcggcgcgg catcaaaatg gttagcttcc
    21661 cgctcattct ccagacgagg caggcgaaaa ttgacctggt tgatgagctg ggtattaaaa
    21721 ctgaccacca gcggtgggga gatatagagc cgttcttcac tattggaaag atgaatcgtc
    21781 ttctcaacgc gaaacactaa ctgatgcggt ccgttatcca gttcaatgct gtccgcaccg
    21841 cgcagcagag agctggaaac cttcttgccg tcgagcacca gaagatcgac atcggttgaa
    21901 agccgcagcg tggtggcaaa aacggatacc ggcaaacata atgcaatcaa ggtggtcacg
    21961 atgccggttt tcataggtag ctcctgtcaa aagaccgccc gaataataat gtgtcaaaat
    22021 ttttctttaa tcatacttac taacatatag acatatttcc cgcttttgct ctcattcatt
    22081 cgtattagct gcatggttgg catgtcggct ttgtcgtaca ctttgttaaa cagtctggag
    22141 gaaataatga aagaaaccga tattgcaggc attttaacgt ccactcacac cattgcgctg
    22201 gtgggggcaa gcgacaaacc cgatcgtccc agctatcgcg tgatgaaata tctactcgac
    22261 caggggtatc acgtcattcc ggtttcgcca aaagttgctg gcaaaacact gctgggccag
    22321 caggggtatg gcacgctggc agaagttcct gaaaaagtcg atatggtcga tgttttccgg
    22381 aattcagaag cggcgtgggg cgtagcgcag gaagcgatcg ccatcggcgc gaaaaccctc
    22441 tggatgcaac tgggggtgat taatgagcaa gcggcagtgc tggctcggga tgccggatta
    22501 aatgtagtga tggatcgctg cccggctatt gagatccctc gcctgggcct ggccaaataa
    22561 aaaatccccg gaaggcaaaa accttccggg gatttgttca gggattagtt acgcagacgc
    22621 ggggcctgga gttgtttacg gatggtctgc gccagctcat ccatggatgg ctgttcagga
    22681 tgttcatcct gcaattcgct acttagctgg gcttcagcca ggtaggtatg aaccggtagg
    22741 ccgttatcgt cctccatcac cacgtgatac cacggagcgg cgcgaagctc gtcattcacc
    22801 gccagctcat caggcgacgg ttcagagagc gaataaaccg ggtcgatatc cacgaccact
    22861 ccgagataac ctaacaggga atggcggacc tgctggccga taccgaattt gctggcaatc
    22921 atagtcacct cccgggaaat ctttaccttt gatataaggg taaaattcca catttcaagt
    22981 tacatgacgc gacaggcaaa ccctttcaga tatagccctt ccggataggt agcgatcacc
    23041 ggatgatcgg ctgcctgacg gaactgctct ataaattgta catcacgacc ggcatcaatt
    23101 gcggcatccg cgatgatttt ctgaaataaa tcgctggtca tcagaccgga acaggagaaa
    23161 gtcaggagaa taccgccttc attcagcagc tgaatcgcca gcatgttgat gtctttataa
    23221 ccacgacacg cgcccatcaa ctggctttta ttctcaacaa acttcggcgg gtccatcacg
    23281 ataacgtcaa atttttcacc gcgatcgcga taagtacgca gcaatttaaa gacatcatca
    23341 cggacaaact cagccttgct cagatccagt ttgttcagct caacgttctg ccgtgcaata
    23401 tccagggctt cctgggaggt atcaacgctg acaacctggc tgcaaccgcc catcagtgcc
    23461 gatacggcga aaccaccggt ataggagaaa cagttaagta cgcgtttatt ttcgacgtag
    23521 cggcgggtag ccaggcggct gtcacgctga tccaggtagt agcccgtttt gtgtccgtgc
    23581 tgaatattca ccagcagttt catgccgtgt tcttcaatcg gcagcagggc aggcggcaac
    23641 tcgccggtga tggggccctg agtcagctcc atcccttctt ttttacgtac cgcgacgtcg
    23701 ctgcgatcgt aaatggcaca ttccgggtac agcgtttgca gggcgctaat taatgccgcg
    23761 cgctgatact ctgcgccagc actcagcagt tgcagcacca gaaaattacc gaaacgatcg
    23821 atagtaatac ccggcaggcc atcagattct ccggcgatta aacgatagct gtcgaggcca
    23881 tctttttgcg ccagccagtc acgccatttt tgtgcttgct gcaaacggcg ggtaaaaaaa
    23941 gcaatgtcga tagattcatt cgggtcaaac gtccagacgc gcgcccggat ttgcgaagct
    24001 ggcgaataag cgccgcgtgc taaccatttt ccctgatgat caacaatatc gatggtttca
    24061 ccgaggctgg ctttaccttc catgcgggca acggccccgg aaaagaccca cggatggcga
    24121 cgaagtaatg atttttcgcg ccctttggct aacactaaac gtacactcat aatttacttt
    24181 tctgtcgatg caaagaaatg ggcgtcattg tccggagttt tccatggatt tgcaacgcgt
    24241 ccattaagga taaggagagc agcaatgtcg aaagtctgca taattgcctg ggtttacggt
    24301 cgagttcagg gcgttggatt tcgctatacc acgcagtacg aagcgaaaaa actgggatta
    24361 gccgggtacg ccaaaaatct tgacgatggc agcgtggaag tggttgcctg cggtgaagaa
    24421 gggcaggtag aaaaattaat gcagtggtta aagagtggcg gcccgcgttc tgcgcgtgta
    24481 gaacgggtgc tcagcgagcc gcatcatccc tcgggggaat taaccgattt tcgtatccat
    24541 taaatacatt ttaccggttt tggcaggcca gcaattttgg tggcttgctt agccggacct
    24601 ttcggaaaca gtcggtataa atagcggctg tttccttttt cttcgccaaa tttattcgcc
    24661 atcgctttta ccagcatacg aatcgccgga gaagtattga attccagata gaaatcacgc
    24721 acaaaacgca ccacttccca gtgttctggc gacagcgtaa tcccttcgtt ctctgcaatc
    24781 accactgcca gcggctcact ccactggctg ctttctttga gatagccttc ggtatccgtt
    24841 tctatctctt taccttcgaa gatcagcata attattacta ccttaatcag actggcggca
    24901 gtgtaacaaa aaacaaagcc ccgcataaag cgaggctatg aaagtgttag cgggtaagat
    24961 taatcgcggc tggcgaagcc cagaatgctc agcaggctga cgaagatgtt gtacagcgaa
    25021 acatacaggc taaccgtggc acgaatatag ttcgtttcac cgccatgaat gatgttgctg
    25081 gtttcaaaca agatagcgcc agaggagatc agaatgaaga ccgcgctgat cgccagatgc
    25141 agagcaggca gttgcaggaa gatattagca accataccaa tcagcaccac cacaataccc
    25201 gccatcagca taccgccgag gaacgacata tctttgcggg tggtcagcac atatgcggag
    25261 cagcagaaga acactaacgc cgttccgccc agtgccatag cgattacgtc acccattccg
    25321 gcagacagat aggtattcag aattggtccg aggatataac ccagaaaacc ggtaaaggcg
    25381 aatgcggaga taatcccggt cggcttattc gccgttttat aggtcaggaa catcaaacca
    25441 tacataccca ccagcgtcag aatcagaccc ggagatggca gcatcagcac cgtactggca
    25501 gttgcggtaa tcgccgaaaa ggccagcgtc aggctcagca gaaaataggt attacgcagc
    25561 accttatggg tgctaagcag cgatgtacgg tcatgtgaag aactaacaat acgatccatt
    25621 gagtcactct ctatgacaga tgtaattaat taagcagcat aatgataatg cgtaagggca
    25681 cccagaagtt ttacccatct ttacgcattt gatctggaac aggtttaaca gcggattatc
    25741 aggttattaa gcaatgatat aacgctctga gaatttcgac agacaaaaga aaaaggggtt
    25801 agcatttagc taacccctta tcttatttgg cggaagcgca gagattcgaa ctctggaacc
    25861 ctttcgggtc gccggttttc aagaccgaag aaaaacaaat aaaaatcagt gtaatacgtg
    25921 aagctgctga aaatttaatc aaatttaaca ccaagaaaaa tcaaggtgtt aggaagttgc
    25981 tcatagctag tataccaagt atttttgcca gaaaattttc tgtattgcct acttgctaac
    26041 cacgagcggc agatccagtg ttggtgtgat ttttgtttta cgatcgtaaa tcaacacctg
    26101 attttctgtt ttatgcccgc tgaaaatttg tttgtcgcga ctgctgcctt cgtaatcaga
    26161 gatccccttg gcttttatat cgtgaaaatt gcacccgaac ggaacgccaa ctttttgctc
    26221 ggctgagcgt ttagcctgat tccaccagtt gttcagcgtt ttagctatga ctttcccgcc
    26281 tttggttgta ttgatcacat attcgcatgt acaggaagat acatttcggg ctaactggat
    26341 cgctgtacgt aatcgcggtg accattcctt gatttgtttg gtgccggttt tgttttgctc
    26401 aatgtagatc cctttatcca taatatcctg ccatttcaac tcaagcacat caccgagcct
    26461 tgccgcacag agataggaaa tctccattgc aatgcgtaac tgtggaattg cttccgcata
    26521 tatcgctgca tactgttcat cggtgatgta aacagtgcgg gctttaagag agaattttct
    26581 gactcctttg catgggttat tcttcacgta tccacgctca tatccccacc cgtatactcg
    26641 actcaggctt gccagttcat gatttgcctg ggtcttgctc tcaagccctc gtttatccat
    26701 gaaaattctt acctgctcag ttttgacatt atcagcaagc acttttccga ataccatcag
    26761 caacgccttc tgatgttgtc gataatcttt ttgggttcgg ggggccagtt ctgtaaatgc
    26821 aggggagtcc ataaacatgt gccataattt agctacggtc attatgttgt ggagttttgc
    26881 tttttccagt tcataatttt gccagacttt agctacgctg gttttccgca ctcttcctag
    26941 ccctatagtt cttgtacttc cttctggttt ccacacgtaa ctgtaaccat tcgatctaac
    27001 ccgcggtggc agtacattat cttttttatt ttttcttggt cttcccattg ttcagcgcct
    27061 caaaatcggg ttcagcagaa accagttcag atgtttttgg catcgttgtc agtccgtgtg
    27121 gaatatccct gcgtagaact attggttcgt ttttaggacc gattacaaat gggatgccgt
    27181 gcagtcttaa ctggtgttgc tgttttgtgt atcgctcgta tttcgtgatc tctttaatct
    27241 ctgctggaga tagagttaat tcgtacatgt ggtcacgtgc ctttacagca tgaccgccgc
    27301 cactatagct ggtgggcggc gatatgggtt gaacattaaa aatcagcctg actcgggatc
    27361 agtttttgcc agatagctga aacgtatttt gcctgataac gggcgtcatc aagtgcatta
    27421 tggcgctcac cttcgaatgg aatagccgtt ctggcatcga agtctatggc tttccccagc
    27481 tcaacgattg tgcgtacatc gcgatcgttg tagtaacgcc acgggcaggg gatcccctgc
    27541 cgttcgtatg aacggcgcaa aatcgtgttg tcgaagttgg ctccatttcc ccagacctga
    27601 acaaaaaatt caccggagtt ttcgtcgata aattcccgca gttgtaacag tgcatcatct
    27661 aacgggattt catcggtcat aatggcagat tgcgcttcgc gtgattgctt aagccaccat
    27721 ttaatggtgt cccgatcaat gactccgcca gcagtttcca gatcgatagt cttactaaat
    27781 tccggtccca tatctccggt ttgcggatcg aaaaatattg cacctattga gatgatcggg
    27841 gcattaggat tttttcccat ggtttcaagg tcgatcatta gatggtcaca cgtcctgctg
    27901 gtggatgtga tttcgtgatg accgttcacc gcaattaagg gatctgccgc ttcgccagtt
    27961 tcactatcgc tggtgtgatg ctgatcgtta ccagcattct cattgttcgg atgttcagcg
    28021 ccttctattt cctccggatc atcttcctga acttcagcct gatttttttc atcgaatgtt
    28081 tcctggtatg ttgcgtcgcc catcaccgcg ccacagtcag gacagttgcc gccaccgctc
    28141 tgaccgcagg cggtgcagac tttttcctct tcctgttgcg ctactggttc ggattgtttc
    28201 gtttctggct cgtcttgtaa cgcattttgg ctgttttgtt ctgctttctg gtcgttctgt
    28261 tccgtttcta gctggttctg gttcacagaa tcgcaggttt caatcccctt aacccatttc
    28321 gaatcattcg ggtcgctaat cccttcaaca aactcaccac gagaagcggc aagcaatttg
    28381 tcggcatctg gtcgactgat gtctgctgcc tccataattt tatttacttc ttcagcggta
    28441 acttttacct gtgacttgtc atccagcggc tgcgggtcct gatgatgttc aactgtatcc
    28501 gctgccattg tttcagccgt tgcctgttca tctgccattg cgccagatgg ttgcgggttt
    28561 tcttcatcat ttttttcttc ttctgttttg cactcagcag ccagtccgtc gttaatttct
    28621 tccaggatat ctttttccgg tgtatgccgg gcagccatga gcgtttcagc tgtgggattc
    28681 tcgtgatcag tttctgtcag gttggcattg atatacccct gaaggcgtcc cgggtagtga
    28741 taaaactcag ggtgggcgct ccggataagt gcaaaaatag ctgcgcggga atagtccagg
    28801 ataccagggg ttgcgcgaag tgctgcggac cattccctga acggactttc ttttttcagg
    28861 acgatttctt ttgcgcgacg ataaacgctc acgggtattt cataaatatt aaaatccatc
    28921 ggaagcgtgg ctgctgcagc ctccacatcc agagtatcga gggtgtgtac taagtcagga
    28981 ttacgatcgg ttttatttcc gccaccggca tttgcaccgg aagccgtgcg ggtgatgcga
    29041 gaaacacgat ttcctttcat ccactctttt gtcagcagac ctcgatcggt atagtcagcg
    29101 tccaggtatg cttcgaaaaa agcagtcatt aaccccagac tggaattaac gggattaggg
    29161 aaaactttgt ctgtgtcgcg taccagttta tgaaggtcgc gaatctccag cgggtcgagc
    29221 agctttgtgt tgtgggaaat agccagggca gtaacagccg gcagttcttc agctcgtgcg
    29281 atatgtaatg cctgaagttc ttcccgtgaa acgtgcgtta ccggtttttc gctgccatgt
    29341 tgcgcaagcc agcgaatggg tagttcctga ccggaaattg gcaggagcat attatcctca
    29401 atctcagcca tgtcttcgcc attgacgttg gtattgtcag tgctggctgg tttttcctga
    29461 actgaggggg aaggcgctat aaatatcatt gtgatgccat cttccccgcc tttttcatag
    29521 cggttgcaga attcagtatc aaacacgcct tccggtggaa ggtcattcac aacggggaaa
    29581 tggacgcgga cgggtttttt gaaatcatca gcgtcgtagc ctgcctcgtc cattgcaaca
    29641 acgcaccgcg agattgctac agataatttc ttttctgtac tccagtaaaa acccccttta
    29701 atcccaaagc gttttctgac cttgtcattt tttgcttcgc aatatagcgc aaattcttct
    29761 ttatcagtgc tcattgataa acctcataac tattttaagg ttgtacgaac ccctgccatt
    29821 gctggcatgc ttaatcaacg ggtatggcgt taatatggct ggtggattat ccagccggta
    29881 tttcgttatt caggtacagc gatacttttt ttaacgggag gcattcgcca gaaatttttt
    29941 gctcgtctct tgcccggagg catgattctt tactggcata aattccggta atcacattct
    30001 gtggctcacc cgttataaga aaaacagtca ttaccagtgc aaatgcagaa gtcactgctg
    30061 ccccccgaaa ataccaagtt taagaagggc aattctggag agtatggaat tatcattgag
    30121 aagataaggc tcatattttc tcatcttaat ggcatcttca gtaaactccc ggttactgag
    30181 cagaacacca atatcaaaac acccttcaga cgtattaacg tttggtaata acgtttccat
    30241 tatcgtgtcc tcaacaatga attttgtgat gtggtgcctg gtgcctccag gtgacgttaa
    30301 ccagttaaca attaacgccg gaatacagaa ggatgcccgt tacgccccgt aaaagaccac
    30361 tttacggatt taactgttcc gcgtgcgcat agccgcattc accgcatcac aaaattcact
    30421 ttaaaaagga tggcagagca gtcatggagt cgaactgatg ccaccaaaga ctacacatgg
    30481 atattgtggc ggggctgtca cttaagcgta tggtcagcct gacaacccgg tgtcctcaag
    30541 gggaaggaat aaccccgcca tacttaccgc cgcgccattt cgcggattgc cacaaccgga
    30601 agcgcacggt cgaattaaat ttaacgacaa agtacagaga gacgagcttc tccgtgcgct
    30661 ttcgtgttat gccctgactt ttcagggtaa attaaccagt ggcaacctgt ttttactggt
    30721 gctaatcagt tagtgtttct ggctaaccag cgacgcgcgc cagcttcggt tttaaacttt
    30781 ttacttttgg tatacgtcat cgcggtaaac gtgccgtcct ggttagggaa cacaccgcat
    30841 accagagatt cgttgttgcc aagattgagc gtatccatgt tgacctcatt tccccttaac
    30901 gccggggtag cggaacaaaa aacctgctgc atagttaaac gtaatccccg ccgtcatgtt
    30961 catacgcctc aggctggcta cttaacccct gaccactgcc gggtaactcg aagtattgcc
    31021 ctgcgttctg tgggtcgggg tgggtgtatg gatacaattt acaaattaaa atttaactgg
    31081 tcaacatgtt tattatta
//
LOCUS       DAZGAP010000035        31051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGAP010000035 DAZGAP010000000
VERSION     DAZGAP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.035"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            329..1054
                     /locus_tag="ACHLAM_001365"
     CDS             329..1054
                     /locus_tag="ACHLAM_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7852924.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            1012..1689
                     /gene="bglJ"
                     /locus_tag="ACHLAM_001366"
     CDS             1012..1689
                     /gene="bglJ"
                     /locus_tag="ACHLAM_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO7852925.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1726..2514)
                     /gene="fhuF"
                     /locus_tag="ACHLAM_001367"
     CDS             complement(1726..2514)
                     /gene="fhuF"
                     /locus_tag="ACHLAM_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO7852926.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2655..2891
                     /locus_tag="ACHLAM_001368"
     CDS             2655..2891
                     /locus_tag="ACHLAM_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO7852927.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     AMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
     gene            complement(2930..3016)
                     /locus_tag="ACHLAM_001369"
     tRNA            complement(2930..3016)
                     /locus_tag="ACHLAM_001369"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2980..2982),aa:Leu,seq:cag)
     gene            complement(3216..4247)
                     /gene="rsmC"
                     /locus_tag="ACHLAM_001370"
     CDS             complement(3216..4247)
                     /gene="rsmC"
                     /locus_tag="ACHLAM_001370"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO7852928.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4350..4763
                     /gene="holD"
                     /locus_tag="ACHLAM_001371"
     CDS             4350..4763
                     /gene="holD"
                     /locus_tag="ACHLAM_001371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO7852929.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4732..5178
                     /gene="rimI"
                     /locus_tag="ACHLAM_001372"
     CDS             4732..5178
                     /gene="rimI"
                     /locus_tag="ACHLAM_001372"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7852930.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5193..5870
                     /gene="yjjG"
                     /locus_tag="ACHLAM_001373"
     CDS             5193..5870
                     /gene="yjjG"
                     /locus_tag="ACHLAM_001373"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO7852931.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5961..7550
                     /gene="prfC"
                     /locus_tag="ACHLAM_001374"
     CDS             5961..7550
                     /gene="prfC"
                     /locus_tag="ACHLAM_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO7852932.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQSAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7943..8548
                     /gene="osmY"
                     /locus_tag="ACHLAM_001375"
     CDS             7943..8548
                     /gene="osmY"
                     /locus_tag="ACHLAM_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO7852933.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8675..8836
                     /locus_tag="ACHLAM_001376"
     CDS             8675..8836
                     /locus_tag="ACHLAM_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO7852934.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8958..10031
                     /locus_tag="ACHLAM_001377"
     CDS             8958..10031
                     /locus_tag="ACHLAM_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO7852935.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            10028..10807
                     /locus_tag="ACHLAM_001378"
     CDS             10028..10807
                     /locus_tag="ACHLAM_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7852936.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(11025..11888)
                     /locus_tag="ACHLAM_001379"
     CDS             complement(11025..11888)
                     /locus_tag="ACHLAM_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO7852937.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(11860..13410)
                     /locus_tag="ACHLAM_001380"
     CDS             complement(11860..13410)
                     /locus_tag="ACHLAM_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO7852938.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13668..14447
                     /gene="deoC"
                     /locus_tag="ACHLAM_001381"
     CDS             13668..14447
                     /gene="deoC"
                     /locus_tag="ACHLAM_001381"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO7852939.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14525..15847
                     /gene="deoA"
                     /locus_tag="ACHLAM_001382"
     CDS             14525..15847
                     /gene="deoA"
                     /locus_tag="ACHLAM_001382"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO7852940.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15899..17122
                     /gene="deoB"
                     /locus_tag="ACHLAM_001383"
     CDS             15899..17122
                     /gene="deoB"
                     /locus_tag="ACHLAM_001383"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7852941.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17179..17898
                     /gene="deoD"
                     /locus_tag="ACHLAM_001384"
     CDS             17179..17898
                     /gene="deoD"
                     /locus_tag="ACHLAM_001384"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO7852942.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18059..18322)
                     /locus_tag="ACHLAM_001385"
     CDS             complement(18059..18322)
                     /locus_tag="ACHLAM_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7852943.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(18354..19370)
                     /gene="lplA"
                     /locus_tag="ACHLAM_001386"
     CDS             complement(18354..19370)
                     /gene="lplA"
                     /locus_tag="ACHLAM_001386"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO7852944.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            complement(19398..20042)
                     /locus_tag="ACHLAM_001387"
     CDS             complement(19398..20042)
                     /locus_tag="ACHLAM_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO7852945.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            20148..21116
                     /gene="serB"
                     /locus_tag="ACHLAM_001388"
     CDS             20148..21116
                     /gene="serB"
                     /locus_tag="ACHLAM_001388"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO7852946.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            21165..22547
                     /gene="radA"
                     /locus_tag="ACHLAM_001389"
     CDS             21165..22547
                     /gene="radA"
                     /locus_tag="ACHLAM_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO7852947.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            22568..23800
                     /gene="nadR"
                     /locus_tag="ACHLAM_001390"
     CDS             22568..23800
                     /gene="nadR"
                     /locus_tag="ACHLAM_001390"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO7852948.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            23892..24224
                     /locus_tag="ACHLAM_001391"
     CDS             23892..24224
                     /locus_tag="ACHLAM_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7852949.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            24226..24510
                     /gene="nadS"
                     /locus_tag="ACHLAM_001392"
     CDS             24226..24510
                     /gene="nadS"
                     /locus_tag="ACHLAM_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO7852950.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPDFFRELASH"
     gene            complement(24566..26233)
                     /gene="ettA"
                     /locus_tag="ACHLAM_001393"
     CDS             complement(24566..26233)
                     /gene="ettA"
                     /locus_tag="ACHLAM_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO7852951.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            26569..28506
                     /gene="sltY"
                     /locus_tag="ACHLAM_001394"
     CDS             26569..28506
                     /gene="sltY"
                     /locus_tag="ACHLAM_001394"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO7852952.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWELSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            28596..28922
                     /gene="trpR"
                     /locus_tag="ACHLAM_001395"
     CDS             28596..28922
                     /gene="trpR"
                     /locus_tag="ACHLAM_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO7852953.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(28916..29431)
                     /gene="yjjX"
                     /locus_tag="ACHLAM_001396"
     CDS             complement(28916..29431)
                     /gene="yjjX"
                     /locus_tag="ACHLAM_001396"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO7852954.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            29483..30130
                     /gene="gpmB"
                     /locus_tag="ACHLAM_001397"
     CDS             29483..30130
                     /gene="gpmB"
                     /locus_tag="ACHLAM_001397"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO7852955.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(30127..30996)
                     /gene="robA"
                     /locus_tag="ACHLAM_001398"
     CDS             complement(30127..30996)
                     /gene="robA"
                     /locus_tag="ACHLAM_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO7852956.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
BASE COUNT         7424 a         7898 c         8426 g         7303 t
ORIGIN      
        1 taaatattga ttgtgtgatt ttgcattctg ctggaaggga tatcagttaa ttataaatta
       61 aatgaatgag aattctgttt ttcattaagt atttctaaca acttaatgta gatattttaa
      121 atgtctccag gctatttcac caggttatca tttgatgcta accttctgac acatgcagtg
      181 gagttgttgt gcagcaggag tatgctgata tgaaagtgaa tgctaaggat aatttattcg
      241 cttaatctat taatttgctg gaaacattta aggaatgaat atcctgaata aaccatcatg
      301 atttttagac agtggatgtg gaggaaatat gttgccagga tgctgcaaaa atggaattgt
      361 tatcagtaaa atacctgtta tgcaagcagg gttaaaagag gtcatgagga ctcacttccc
      421 tgaatatgaa ataatatcca gcgcctctgc ggaggacctt accttattac aattacgtcg
      481 ttccggatta gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga
      541 acattattat tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg
      601 ctcctggtat tcccaggcag tagaactgct tatgtgccct acggcgacgt tattgtctga
      661 tgttgaaccc attgagaatc tggtcaagac cgtacgttcc ggcaatacgc acgcagagcg
      721 tatcagcgcc atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc
      781 cgtcattctc actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat
      841 caaccagata gcttcattgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag
      901 tgcgatgcgt cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaatagcgc
      961 gcagggtgca agagaactta acttgccttc tgtttatgga gatgccgcag aatggaacac
     1021 agccgaatta agaagagaaa tgtcgcactc atagaaaaat gcgtcatgag cagtatcggt
     1081 attgagagtt tattcagaaa gtttgcgggt aacccttata agctccatac ctataccagt
     1141 caggagtcat ttcaggatgc catgtcgcgg atctcgtttg cggcggtcat tttttctttt
     1201 tctgccatga gaagtgagcg cagagaggga ttatcttgcc tgactgaact ggcgattaag
     1261 tttccgcgta cccggcgttt agttattgcg gatgatgata ttgaagctcg gctgattggt
     1321 tcattgtcgc catcaccgtt ggacggtgta ttaagtaaag cgtcaacgct ggagattttt
     1381 catcaggaac tctttttgtc attaaatggt gtacgtcagg cgaccgaccg actgaacaat
     1441 cagtggtaca ttaatcaaag ccgaacgcta agcccgacgg agagagaaat attgcgcttt
     1501 atgtcgcgtg gctactcaat gacacaaatt gccgagcagc ttaaacgcaa tatcaaaacg
     1561 atccgtgcac ataaatttaa tgtgatgtcg aaactgggcg tcagttctga tgcagggttg
     1621 ttggaggccg cagatattct gttatgtatg cggcattgcg aagcaagtaa tgtgttgcat
     1681 ccctattaat ccacatgatg ccgggtttac ttccccggca gtgcttcatt tcagcgtaca
     1741 atcgccacat tgctgcacat ccggtaagcg ataacgctgg cagcaagtgc ggcgcaccag
     1801 caggccgtcg cgcagtacca cggtacgcca gagtggatta tcttcaccgt tcgtgagcat
     1861 tttctcaaaa aagagggcat ggcgcagcga ttcaaccgtc gcctcgccaa gcagctgttt
     1921 catctcagtc agataccagt tgatcaaata accggtattg ctccagataa gtttgccgtt
     1981 gatctctccc gtcgcttcta atgcctgcac taccggaaca agcgcctggc tgattaacgt
     2041 ttccattcgc tgctgcggcg aatgtagtgt tgcgttttta tcttcacaca catcgaccca
     2101 gaaacaggcg gcgcgtccgg tttcgtgaaa ctcaacgtgg aaatgttccg gcgacacatc
     2161 taatgctttt tcctgcgtca gcagcgccaa cattaatggt ggcaccatca ggccgatata
     2221 ccattgtgcc catagtgaga tcagcggttt gttctcgcgg atcatcaagg gttggttgcg
     2281 gtagatatga tcggaataga ccgccagttg agaacttagc gtattcggtg atgaccattg
     2341 cgccagcgtc atggcacgga gcggtgcggg ttcatccaga cggataaact ccagcaaatg
     2401 ttcacgatgt tcggcgatcg tcgcccgtac ggcttgtgca agcccagcat cctgcggctg
     2461 gagatgcgtt cgccagataa catcttcata gagcggtgcg gaacgatagg ccataatcgt
     2521 gatagtaatc taaatgataa tgattgctaa tcctagcgat aggtttaccc gatagcaagg
     2581 gatttctctg gcttgcaaat gataaaaatt atcatatgat attggttatc attatcaatg
     2641 aaagagatga aaccatgttg caacgtacgc tgggcagtgg ctggggagtg ttgctgccgg
     2701 gattgctgat tgcagggctg atgtatgcgg atttatcgcc ggatcaatgg cggattatca
     2761 ttctgatggg attagtattg acgccggcga tgctgtatca caaacagttg cggcattacg
     2821 ttttgctgcc atcgtgcctg gcgcttattg ctggcatcat gctgatgata atgaatttga
     2881 agcagggatg ataaaacagg gaagaaacaa gaaaggaagt aaagataatt ggtgcgaggg
     2941 gggggacttg aacccccacg tccgtaagga cactaacacc tgaagctagc gcgtctacca
     3001 attccgccac cttcgcatac cgtcaattct taaaaagaat tgctaccacg gaggcgcatt
     3061 ctagtggttt tcagcttttc gtcaatagtt aattatcgac agaggtgtaa ttgctggaaa
     3121 aatgtccatc agcggattgt ggataattcg tttgtcggtc agataaggcg tgtacgccgc
     3181 atccgacacg gtatgcggcg acatatatca gatactcaac ctttcttcgc ctggcgggtc
     3241 ataatggcgc gatacacctt gaagcgcccg gtttgcgcga tcacttcgtg gaagccaaat
     3301 gtctcatcca gcacgtccgg ataaggcagg aaggcgttcg ctacaattcg cagctcgccg
     3361 ccgctattaa gatgacgcac cgcgccgcga atcagcgttt gcgcggcatc caggctggtt
     3421 tgcatcccat cgtggaacgg cgggttggag atgatcatat caaaacgacc tttcacctcg
     3481 gaaaagacgt tactggcaaa gacttcacct tcaacaccgt tggccgcaag tgttgcgcgg
     3541 ctggcttcta ccgctggtgc agagacatcg cacagagtga gacgaatttt cggcgaatgg
     3601 cgcgcaaagg caaccgaaag tacgcccgcg ccacagccga catccagcac tttacctttc
     3661 gtgtgcgggg ttaacgtcga gagcagcaac tggctaccga catccagacc gtcgcggcta
     3721 aacacgccag gcagcgtttt gaccgtcagg ccatcgacgc tgtattcgcc ccagaattta
     3781 tcggcatcaa atactggctg tttttccaga cgaccaaaat agaggccaca gcgacgagcg
     3841 ctgtccattt tattcaacgg cgcataatct gacagcatct gctcggcgct gcgcacgccg
     3901 ctgcggttct cgccaacgac aaaaatatcc gtgcccactg gcagcagaga aagtaaattc
     3961 atcaactgga actgggcttc cggtttgttc ttcggccagt agtaaatcag cgtatcgcaa
     4021 tctgcgacat catccgccgt ggcgaccaga ctaaaacggg cgttatcccc catctggcgg
     4081 cttaacacct gccagtggtg gaattgctgg gtatgagcac ggctggccgc ggtatctaaa
     4141 cgcgcgggca ggtcatcctg taagtctccg gcaaaaagaa tacggctttg ttcgaaatca
     4201 tcactgtgac gcagcaagac ttcacttgcc ggggtaaatg cagacatgga atgctcctca
     4261 attaatactg gcggcgatta tagccatatg ttggcgcggt atcgacgaat ttgctatatt
     4321 tgcgcccctg acaacaggag cgattcgcta tgacatcccg acgagactgg cagttacagc
     4381 aactgggcat tacccagtgg tcgctgcgtc gccctggcgc gttgcagggg gagattgcca
     4441 ttgcgatccc ggcacacgtc cgtctggtga tggtggcaaa cgatcttccc gccctgaccg
     4501 atcctatagt gagcgatgtt ctgcgcgcgt taaccgtcag ccccgatcaa gtgctgcaac
     4561 tgacgccaga aaaaatcgcg atgctgccgc aaggcagtcg ctgcaacagc tggcggttgg
     4621 gtactgacga accgctatca ctggaaggcg ctcaggtggc atcaccggcg ctcaccgaat
     4681 tacgggcaaa cccaacggca cgcgccgcgt tatggcaaca aatttgcaca tatgaacacg
     4741 atttcttccc tcgaaacgac tgatttacca gcggcttacc acattgaaca acgcgcccac
     4801 gcctttccgt ggagtgaaaa aacctttgcc agcaaccagg gcgagcgtta tctcaacttt
     4861 cagttaacgc aaaacggcaa aatggcggcg tttgcgatta cgcaagtggt gctggatgaa
     4921 gctacattgt tcaatattgc ggtcgatcct gactatcagc gtcagggatt gggaagggcg
     4981 ctgctggaac atctgatcga cgaactggaa aaacgcggcg tggcgacgct atggctggaa
     5041 gtccgtgctt caaacgctgc cgccattgcc ctgtacgaaa gtttaggctt taacgaggcg
     5101 acaattcgcc gcaattacta ccccaccacg gacggtcgcg aagacgccat catcatggcg
     5161 ttgccaatca gtatgtaaga caaggtggaa taatgaagtg ggactggatt ttctttgatg
     5221 ccgatgaaac gctgtttacc tttgactcat tcaccggctt gcagcggatg tttcttgatt
     5281 acagcgtcac ctttaccgct gaagattttc aggactatca ggccgttaac aagccactgt
     5341 gggtggatta tcaaaacggc gcgatcactt cattacagct tcagcacggg cggtttgaga
     5401 gctgggccga acggctgaac gtcgagccgg gtaaactcaa cgaagccttt attaatgcga
     5461 tggcggaaat ctgtacgcca ctgccgggcg cggtttctct gcttaacgcc attcgtggca
     5521 acgctaaaat tggcatcatc accaacggct ttagcgcctt gcagcaagtg cgtctggaac
     5581 gcacgggcct gcgtgattat ttcgatttgc tggtgatttc cgaagaagtt ggcgttgcca
     5641 aaccgaataa gaaaattttc gattatgcgc tggaacaggc aggcaatcct gaccgttcac
     5701 gtgtgctgat ggttggcgac acagctgagt ccgatattct cggtggcatc aacgccgggc
     5761 ttgcgacctg ctggctgaat gcgcacaatc gcgagcaacc agaaggcatc gcgcccacct
     5821 ggaccgtttc ttcgttgcac gaactggagc agctcctgtg taaacactga ttgcctcccc
     5881 cccgttgatg ggtaaaatag ccgcaatttt tcgttttcaa caagcgcggc gcgatgccgc
     5941 ttactcaaga agaaagaatt atgacgttgt ctccttattt gcaagaggtg gcgaagcgcc
     6001 gcacttttgc cattatttct cacccggacg ccggtaagac taccatcacc gagaaggtgc
     6061 tgctgttcgg acaggccatt cagagcgccg gtacagtaaa aggccgtggt tccaaccagc
     6121 acgctaagtc ggactggatg gagatggaaa agcagcgtgg gatctccatt actacgtctg
     6181 tgatgcagtt tccgtatcac gattgcctgg ttaacctgct cgacaccccg gggcacgaag
     6241 acttctcgga agatacctat cgtaccctga cggcggtgga ctgctgcctg atggttatcg
     6301 acgccgcaaa aggtgttgaa gatcgtaccc gtaagctgat ggaagttacc cgtctgcgcg
     6361 acacgccgat cctcaccttt atgaacaaac ttgaccgtga tatccgcgac ccgatggagc
     6421 tgctcgatga agttgagaac gagctgaaaa tcggctgcgc accgatcacc tggccgattg
     6481 gctgcggcaa gctgtttaaa ggcgtttacc acctttataa agatgaaacc tatctctatc
     6541 agagcggtaa aggccacacg attcaggaag tccgcattgt taaagggctg aataacccgg
     6601 atctcgacgc tgcggttggt gaagatctgg cacagcagct acgtgacgaa ctggagctgg
     6661 tgaaaggcgc gtctaacgag ttcgacaaag agctgttcct tgcgggcgaa atcactccgg
     6721 tattcttcgg tactgcgctg ggtaacttcg gcgttgatca tatgctggat ggcctggtgg
     6781 agtgggctcc tgcgccgatg ccgcgtcaga ctgatacccg taccgtagag gcgagcgaag
     6841 acaaatttac cggcttcgta tttaaaattc aggccaacat ggacccgaaa caccgcgacc
     6901 gcgtggcgtt tatgcgtgtg gtgtccggta aatatgaaaa aggcatgaag ctgcgccagg
     6961 tgcgtactgc gaaagatgtg gtgatctccg acgcgctgac ctttatggcg ggtgaccgtt
     7021 cgcacgttga agaagcgtat cctggcgata tccttggtct gcacaaccac ggcaccattc
     7081 agatcggcga cacctttacc cagggtgaga tgatgaagtt caccggtatt ccgaacttcg
     7141 cgccagaact gttccgtcgt atccgcctga aagatccgct gaagcaaaaa cagctgctta
     7201 aagggctggt acagctttcc gaagagggcg cggtgcaggt gttccgtccg atctccaaca
     7261 acgatctgat cgttggtgcg gttggtgtgc tgcagtttga tgtggtggta gcgcgtctga
     7321 agagcgaata caacgttgaa gcagtgtatg agtcagtcaa cgttgccact gcccgctggg
     7381 tagaatgtgc ggacgcgaag aaattcgaag agttcaagcg taagaacgaa agccaactgg
     7441 cgcttgatgg cggcgataac ctcgcttaca tcgctaccag catggtcaac ctgcgcctgg
     7501 cgcaggaacg ttatccagac gttcagttcc accagacccg cgagcattaa ttcttgcctc
     7561 cagggcgcgg tagccgctgc gccctgtcaa tttcccttcc ttattagccg cttacggaat
     7621 gttcttaaaa cattcacttt tgcttatgtt ttcgctgata tcccgagcgg tttcaaaatt
     7681 gtgatctata tttaacaaag taatgacatt tctgacggcg ttaaataccg ttcaatgcgt
     7741 agatatcagt atctaaagcc gtcgattgtc attctaccga tattaataac tgattcagag
     7801 gctgtaatgg tcgttattca tcacccatcg cttttgtgat ggcggccatt gacttctgta
     7861 gagggtgaag tctctcccta ttcagcaatg caacctcgtg ttgccaggct caaattacga
     7921 gcaaacatac aggaataaat cgatgactat gacaagactg aagatttcga aaactctgct
     7981 ggctgtaatg ttgacctctg ccgtcgcgac cggctctgcc tacgcggaaa acaacgcaca
     8041 gactaccaat gaaagcgcag ggcaaaaagt cgatagctct atgaataaag tcggtaattt
     8101 catggatgac agcgccatca ccgcgaaagt gaaggcggcg ctggtggatc atgacaacat
     8161 caagagcacc gatatctctg tcaaaaccga tcaaaaagtc gtgaccctga gcggtttcgt
     8221 tgaaagccag gctcaggccg aagaggcagt gaaagtggcg aaaggcgttg aaggggtgac
     8281 ctctgtcagc gacaaactgc acgttcgcga cgctaaagaa ggctcggtga agggctacgc
     8341 gggtgacacc gccaccacca gtgaaatcaa agccaaactg ctggcggacg atatcgtccc
     8401 ttcccgtcat gtgaaagttg aaaccaccga cggcgtggtt cagctctccg gtaccgtcga
     8461 ttcccaggca cagagcgacc gtgctgaaag tatcgccaaa gcggtagatg gtgtgaaaag
     8521 cgttaaaaat gatctgaaaa ctaagtaatt cgtcgtaatt cgtcctcccg aactttgtcg
     8581 ggaggcgcga tgtgcaccac actaaaaata tcgcattgag tgattttcac gctcatatca
     8641 agcggtgaca ttaactatgg taaaggagac gcttatgttt cgttggggca tcatatttct
     8701 ggttatcgcg ttaatcgccg ccgcacttgg gtttggtggt ctggccggta ccgctgcagg
     8761 cgcagctaaa attgtctttg tcgtcgggat tattctgttc ctggtgagtt tgttcatggg
     8821 ccgaaaacga ccctagattt cagaccatta agaaaatttt atccaaagcc agtccagcgg
     8881 actggctttt gcggttttag cgaataataa attgcgttac tttgtcattc tttcacaaca
     8941 aaaacaggaa ggcagaggtg gggcagcgaa tacctgtaac gcttggtaat attgcgccgt
     9001 tgtcgctaag gccgttccag cctggacgaa tagctctggt gtgcgaaggc ggcggacagc
     9061 gtggaatttt cacggctggc gtgctggatg agtttatgcg cgcgcagttt aatcctttcg
     9121 atctttatct cggcacatct gccggggcgc agaacctctc ggcgtatatc tgcaatcagc
     9181 ccggttacgc gcgcaaagtc atcatgcgct ataccacaaa acgcgaattt ttcgatccat
     9241 tgcgctttgt ccgtggagga aatcttatcg atctcgactg gctggtggag gccactgcaa
     9301 gccagatgcc gttgcaaatg gataccgccg cgcggttgtt tgacagcggc aaatcgtttt
     9361 atatgtgcgc ctgtcgtcag gatgactatg cgccgaatta ctttttacca accaaacaaa
     9421 actggctgga tgtgattcgc gcctccagtg cgatacctgg cttttatcgt agcggagtgt
     9481 cgctggaagg cattaactac ctggatggcg ggatcagtga tgcgattccg gttaaagagg
     9541 cggcaaggca gggagcaaaa accttggtcg tcatccgcac cgtgccgtcg caaatgtact
     9601 acacgccgca gtggttcaaa cgcatggaac gctggctggg tgacagtagc ctgcagccgc
     9661 tggtcaatct ggtgcagcat catgaaacca gctatcgtga cattcagcaa tttattgaga
     9721 aaccaccggg ccagctgcgg atattcgaaa tttatccgcc gaagccgttg catagtatcg
     9781 cgcttggcag tcggattccg gcgctgcgtg aagactataa acttgggcgt ttatgcggtc
     9841 gttattttct ggcaacggtc ggtaagttat tgactgaaaa agcgccgctt acccgccatc
     9901 tggtgccggt ggtgacgccg gaatcgattg tcattccgcc tgcgccagtc gccaacgata
     9961 cgctggttgc cgaagtgatc gacgctccgc aggcgaacga cccgacattt aacaatgagg
    10021 atctggcttg atttgccgtt ttatcgacac ccactgccat tttgatttcc cgccttttag
    10081 tggtgatgaa gaggccagcc tgcaacgcgc ggcacaagcg ggcgtaggca agatcattgt
    10141 tccggcgaca gaggcggaaa attttgcccg cgtgcaggcg ttagcggaaa aatatcaacc
    10201 gctgtatgcc gcattgggct tgcatcctgg tatgttgaaa aaacatagcg atgtgtctct
    10261 tgatcagcta cagcaggcgc tggaaaggcg tccggcgaag gtggtggcgg tgggggagat
    10321 cggtctggat ctcttcggcg acgatccgca atttgagagg cagcagtggt tactcgacga
    10381 acaactgaaa ctggcgaaac gctacgatct gccggtgatc ctgcattcac ggcgcacaca
    10441 cgacaaactg gcgatgcatc ttaaacgcca cgatttgcca cgcactggcg tggttcacgg
    10501 tttttccggc agcctgcaac aggccgaaag gtttgtactg ctgggctaca aaatcggcgt
    10561 aggcggtact atcacctatc cacgcgccag taaaacccgc gatgtcatcg caaaattacc
    10621 gctggcatcg ttgttgctgg aaaccgacgc gccggatatg ccgctcaacg gctttcaggg
    10681 gcagcctaac cgcccggaac aggctgcgcg ggtgtttgac gtactttgcg agttacgccc
    10741 ggaaccagaa gatgagattg ccgaagtgtt gctaaataat acgtatacgt tgtttaacgt
    10801 gccgtaggcc ggataaggcg ttcacgccgc atccggcagt tggcgcacaa tgcctgatgc
    10861 gacgcttgac gcgtcttatc atgcctacaa gtctgtgccg aaccgtaggc cggataaggc
    10921 gttcacgccg catccggcag ctggcgcaca atgcctgatg cgacgcttga cgcatcttat
    10981 catgcctaca ggtctgtgcc gatccgtagg ccgtatccgg catgtcacaa atatagcgcc
    11041 ggaaatatta accggctcac cccgcgctcc cataacgcat cagccagttg ttccacatcc
    11101 tccggcgtgg cgctcggcca gctttgcgcc tcgccataca cgccgtgggc atgaaacgcg
    11161 ttcaggcgta ccggaacatc gccaagtccc ttgattaacg ccgccagttc ttcgatgtgt
    11221 tgcaaataat ccacctggcc agggattacc agcaaacgca gttccgccag cttaccgcgc
    11281 tctgccagca aacagatgct gcgcttgatc tgctgattat cgcgtccggt taattgctga
    11341 tgacattcgc tcccccacgc tttgagatcg agcattgcgc cgtcgcacac cggaagcaat
    11401 ttttcccagc cggtttcgct caacatgcca ttactgtcca ccagacaggt gaggtggcgc
    11461 agttgcggat cgtttttgat agcagtaaac agcgccacca caaacggtag ctgggtcgtg
    11521 gcttcgccgc cgcttaccgt tatcccttcg ataaacagca ctgctttgcg gatatggcta
    11581 agcacctcat ccacgctcat ggactgcgcc attggcgtgg catgttgcgg acacatcttc
    11641 aggcaggtat cacattgctc gcagaccaca gcgttccaca ccactttgcc gtcgacaatc
    11701 tgcaacgcct gatgcggaca ctgcggcacg cactctccgc aatcattaca gcgccccatc
    11761 gtccacggat tgtgacagtt tttgcagcgc agattgcagc cctgcaaaaa cagagccaga
    11821 cgactgcctg gcccgtcaac gcaggagaag gggataatct tactgactaa agcgcatctg
    11881 ctgttcatgg cttatcacgc gcggctggcg ttccagaata cgagtgttgc gtgcggcttc
    11941 ttctcccagc caggtggtgt tggtgcgtga accttcggcg cgatattttt ctaaatccga
    12001 caaacgcacc atataaccgg taacgcgaac cagatcgtta ccgctgacat tggcggtaaa
    12061 ttcacgcatt ccggctttaa aggcaccgag gcaaagctgt accagcgcct gcggattacg
    12121 tttgatggtt tcgtcgagcg tcagaatgtc gctgatgccg gaataataat aagcatgatg
    12181 cggggcgaca gtttgcagat gggtaatcgg atctggctca tcaccatacg gcaagcgtgc
    12241 gccaggcgtg gtgccgatat cggaactgat ccccgactgt gcgtgtagca tggcgcgttt
    12301 ttgccagcca tatttcacgg gggtattggc gacaaactcc gccagttgtg cgctgatgcg
    12361 ataacccact tcatttgcgg cggcttcttt accgtagcgc gcggcaatcc cttctttctc
    12421 acacagcaag ttaactgctt ccgccaatcc gtacatgcca aacattggca caaaacgttc
    12481 agggttaatc agcccttctt tcaccaggaa gctattctca aagaagtgtg attgttgata
    12541 gaggaattca caccgcgcat cgatgatggc gatctgctgc tggcagtagt gcggtagagt
    12601 gcgtgtaaag aagtcatcca gcgattcact gcgctcggca atggctttca ggttaaggcg
    12661 taccagcgtg ctgccaccac ccgccagcgg cagtgagttg taacagctca caatcccgta
    12721 gccccctttt gtgaaaattt tatcatgcac cggaccgttg gcgatgtgcg gtttgctaca
    12781 ttcacagatg ttcttcgcca cttccagcag taggtcatca ggggtgattt caggatcgta
    12841 gataaaggtc aggttcggtg aaacctgctt caactctgcg tctgcacgta agatcgcgcg
    12901 ggtaatgggc gaatcagacg ggccgatatt ggcgtgcata aaggcgtctg gcagggttct
    12961 gtcgaggtaa cgccagaaac gttttattcg aatatcgatc tcgtcttgtg ttagaattct
    13021 aacatacggt tgcaacaacg catccagttg ccccaggtag accggcatcg atgtgaccga
    13081 tggtacgtgg tggtaaagaa tggtcagcag agagagtgca tcatcaagat ctttcgcgcc
    13141 ttccagctcc agccattcgg aaccgttcgc cagaaaacgg gcgtaatcgg gtaagacata
    13201 gcgcggtttg tacggcgcat gaccttcaaa catatcgcag attacacctt catccagcgc
    13261 gcggcgggct tcggcaggaa gctgtgggta aggcagattg ttttctgctt ccagtgccag
    13321 aaaatggcgc ttctgctccg ggctaagtac cgggctggtg acaatttgct ggcaacgttg
    13381 ttgcagtgca ttttcatgag aagtgggcat cttcttttcc ttttatgccg aaggtgatgc
    13441 gccattgtaa gaagtttcgt gatgttcact ttgatcctga tgcgtttgcc accactgacg
    13501 cattcatttg aaagtgaatt atttgaacca gatcgcatta cagtgatgca aacttgtaag
    13561 tagatttcct taattgtgat gtatatcgaa gtgtgttgcg gagtagatgt tagaatacta
    13621 acaaactcgc aaggtgaatt ttattggcga caagccagga gaatgaaatg actgatctga
    13681 aagcaagcag cctgcgtgca ctgaaattga tggacctgac caccctgaat gacgacgaca
    13741 ccgacgagaa agtaattgct ctgtgtcatc aggccaaaac tccggtcggc aataccgccg
    13801 ctatctgtat ctatcctcgc tttatcccaa ttgctcgcaa aacgctgaaa gagcagggta
    13861 ccccggaaat ccgtatcgct acggtaacca acttcccaca cggtaacgac gacatcgaaa
    13921 tcgcgctggc agaaacccgt gcggcaatcg cctacggtgc tgatgaagtt gacgttgtgt
    13981 tcccgtaccg cgcgctgatg gcgggtaacg agcaggttgg ttttgacctg gtgaaagcct
    14041 gtaaagaggc ttgcgcggca gcgaatgtac tgctgaaagt gatcatcgaa accggcgaac
    14101 taaaagacga agcgctgatc cgtaaagcgt ctgaaatctc catcaaagcg ggtgcggact
    14161 tcatcaaaac ctctaccggt aaagtggctg tgaacgcgac gccggaaagc gcgcgcatca
    14221 tgatggaagt gatccgtgat atgggcgtag aaaaaaccgt tggtttcaaa ccggcgggcg
    14281 gcgtgcgtac tgcggaagat gcgcagaaat atctcgccat cgccgatgaa ctgttcggtg
    14341 ctgactgggc tgatgcgcgt cactaccgct ttggtgcttc cagcctgctg gcaagcctgc
    14401 tgaaagctct gggccacggc gacggtaaga gcgccagcag ctactaaggc ttgatttgcc
    14461 ggatgatgca gcgatgctca tccggttccc cgattacctc gtctcttttc cacgggaggt
    14521 taccttgttt ctcgcacaag aaattattcg taaaaaacgt gatggtcatg cgctgagcga
    14581 tgaagaaatt cgtttcttta tcaacggtat tcgcgacaac actatctccg aagggcagat
    14641 tgccgccctc gcgatgacca ttttcttcca cgatatgaca atgcctgagc gtgtctcgct
    14701 gaccatggct atgcgagatt caggaaccgt tctcgactgg aaaagcctgc atctgaatgg
    14761 cccgattgtt gataaacact cgaccggcgg cgtcggcgat gtcacttcgc tgatgttggg
    14821 gccgatggtc gcagcctgcg gcggctatat tccgatgatc tccggtcgcg gcctcggtca
    14881 tactggcggt acgctcgaca aactggaatc catccctggc ttcgacattt tcccggatga
    14941 caaccgtttc cgcgaaatta ttaaagacgt cggcgtggcg attatcggcc agaccagctc
    15001 actggctccg gcggataaac gtttctacgc gacccgtgat attaccgcaa ccgtggactc
    15061 catcccgctg atcactgcct ctattctggc gaagaaactg gcggaaggtc tggacgcgct
    15121 ggtgatggac gtgaaagtag gtagcggcgc gtttatgccg acctacgaac tctctgaagc
    15181 ccttgccgaa gcgattgttg gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct
    15241 caccgatatg aatcaggtac tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc
    15301 ggtgcagttc ctgacgggtg aatatcgtaa cccgcgtctg tttgatgtca cgatggcact
    15361 gtgcgtggag atgctgatct ccggcaaact ggcgaaaaat gacgccgaag cgcgcgcgaa
    15421 attgcaggcg gtgctggaca acggtaaagc ggcagaagtc tttggtcgta tggtcgcggc
    15481 acaaaaaggc ccgaccgact tcgttgagaa ctacgcgaag tatctgccga cagcgatgct
    15541 gacgaaagca gtctatgctg ataccgaagg gtttgtcagt gaaatggata cccgcgcact
    15601 ggggatggca gtggttgcaa tgggcggcgg acgccgtcag gcatctgaca ccatcgatta
    15661 cagcgtcggc tttactgata tggcgcgtct gggcgaccag gtagacggtc agcgtccgct
    15721 ggcagttatc cacgcgaaag acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc
    15781 ggcaattaaa cttgccgata aagcaccgga aagcacacca actgtctatc gccgtatcag
    15841 cgaataacgg tatactgatc tgatcattta aatttgaagc actgagtacg gagaacatat
    15901 gaaacgtgca tttattatgg tgctggactc attcggcatc ggcgctacag aagatgcaga
    15961 acgctttggt gacgtcgggg ctgacaccct gggtcatatc gcagaagctt gtgccaaagg
    16021 cgaagctgac cacggtcgta aaggcccgct caatctgcca aatctgaccc gtcttgggct
    16081 ggcgaaagca cacgaaggtt ctaccggttt cattccggcg ggaatggatg gcaacgctga
    16141 agttatcggc gcgtacgcat gggcgcacga aatgtcatcc ggtaaagaca ccccgtctgg
    16201 tcactgggaa atcgccggtg tcccggttct gtttgagtgg ggatatttct ccgatcacga
    16261 aaacagcttc ccgcaagagc tgctggataa actggttgaa cgcgctaatc tgccgggtta
    16321 cctcggtaac tgccactctt ccggtacggt cattctggat caactgggcg aagagcacat
    16381 gaaaaccggc aagccgattt tctatacctc cgctgactcc gtgttccaga ttgcttgcca
    16441 cgaagaaacc tttggtctgg ataaactcta cgaactgtgc gaaatcgccc gtgaagagct
    16501 gaccaacggc ggctacaaca ttggtcgtgt tatcgctcgt ccgtttatcg gcgacaaagc
    16561 cggtaacttc cagcgtaccg gtaaccgtca cgacctggct gttgagccgc cagcaccgac
    16621 cgtgctgcag aaactggttg atgaaaaaca cggccaggtg gtttcggtcg gtaaaattgc
    16681 agacatctat gccaactgcg gtatcaccaa aaaagtgaaa gcgaccggcc tggacgcgct
    16741 gtttgacgcc accatcaaag agatgaaaga agcgggtgat aacaccatcg tcttcaccaa
    16801 cttcgttgac ttcgactctt cctggggcca ccgtcgcgac gtcgccggtt atgctgcggg
    16861 tctggaactg ttcgaccgcc gtctgccgga gctgatgtct ttgctgcgcg atgacgatat
    16921 tctgattctc actgctgacc acggttgtga cccgacctgg accggtactg accacacgcg
    16981 tgaacacatt ccggtactgg tttacggccc gaaagtgaaa ccaggttcgc tggggcatcg
    17041 tgaaaccttc gcggatatcg gccagactct ggcaaaatat tttggtactt ctgatatgga
    17101 atatggcaaa gccatgttct gatggatttg ggcggagcag tgactccgcc tttaatatgt
    17161 cacaaaaagg ataaaacgat ggctactcca cacattaatg cagaaatggg cgattttgct
    17221 gacgtagttt tgatgccagg cgacccgctg cgtgcgaagt atattgctga aactttcctt
    17281 gaagatgccc gtgaagtgaa caacgttcgc ggtatgctgg gcttcaccgg tacttataaa
    17341 ggccgcaaaa tttccgtaat gggtcacggt atgggtatcc cgtcctgctc catctacacc
    17401 aaagaactga tcaccgattt cggcgtgaag aaaattatcc gcgtaggttc ctgtggtgct
    17461 gttctgccgc acgtaaaact gcgtgacgtc gttatcggta tgggtgcctg caccgattcc
    17521 aaagttaacc gcatccgttt taaagaccat gactttgccg ctatcgctga cttcgacatg
    17581 gtgcgtaacg cggtagacgc ggcaaaagca ctgggcgttg atgctcgcgt tggtaacctg
    17641 ttctccgctg acctgttcta ctctccggac ggcgaaatgt tcgacgtgat ggaaaaatac
    17701 ggcatcctcg gcgtggaaat ggaagcggct ggtatctacg gcgtcgctgc agaatttggc
    17761 gcgaaagccc tgaccatctg caccgtgtct gaccacatcc gcactcacga gcagaccact
    17821 gccgctgagc gtcagaccac cttcaacgac atgatcaaaa tcgcgctgga atccgttctg
    17881 ctgggcgata aagagtaatt gtgttttgct gaaaggcgat tgtcgtgtga agccggagtg
    17941 ggagactgct ccggcttttt agtatttatt catcaatagc aactgattta tagatttact
    18001 attatttttc atatggatag aaaataaaat ccattcaagt atctattcac gaatctattc
    18061 atttatgttc agccgtcatc caggaagggc atatcagcaa ctctggtgga caaccataaa
    18121 ttcctgccat tttgcttttg atgtttgaac taagccgttc cgtcttttcg atctgtttta
    18181 gagaattaat ggagatacct aacattttac ttacttcagc aagtgatagt ttatgcctga
    18241 tacgcacggc ggttagccag gatataccct ggctgaacat taaattgata atctcccgtt
    18301 cctggttttc cagatcgatc atatttcctt ccctggatat tttaatcagc gagttacctt
    18361 accgcccctg ccatccacgc tgacaactcc cgtagctctt tttcctgttc cgggaagtca
    18421 accagcagcg cttcgcactc ctgttgcagc atatccgcgc ggtacagaca gccttgcagt
    18481 cgcccggcga gggcttccag cggcgcgggg ttgagactgt cggtaaacac ctgggcgcgg
    18541 gtgatatggc ctttttcaac gtcgaaatgc agttccacgc cgccccaggt aaagcgttca
    18601 tccagcagat gcgagaatgc cggagcctga ccgaagttcc attcccagct actctggcgg
    18661 gcaaaggttt cggcgaagtt tggcaagtct ggcgttttgt ccggggagat gatttccgct
    18721 tccacgcgct cgccataatg ggcgaaaaag gcctcggtta tggcctcgca aacctgctca
    18781 tgggtgatcc ccggtagcaa ttcggtcaga ttggtcacgc gggaacgcac tgaggtaatg
    18841 cctttcgccg ccagtttctt tttatccgga ttgagatagt ttgccaggcg gctaaggtcg
    18901 gaattgagca gcaaagtgcc gtggtggaag ccacgatctt tggtttcgcg ataggccgag
    18961 cctgagactt tgcgatcgcc ttcggcggtt ttcaccacca gatcgttacg cccggaagct
    19021 tcggcgctga cgccgagcgc gttcagcgca ttgagcacaa tcgacgtgga gatggtttta
    19081 tcgtactccg gcttgccagc cataaaggta aaacaggtat tgccgagatc gtggaacact
    19141 gcgccgccac cgctactgcg ccgcgccagg cggacgttat cttcttccat ccgccgggta
    19201 ttacactctt tccacgggtt ctgcgcgcga ccaatcacta ccgtgtcggc attgcgccag
    19261 agaaacagaa cgcgctgcgt agccggcatc tggcgaaaaa tacactcctc taccgccagg
    19321 ttaaaccacg ggtcgtaaga gtcagagatg agcaggcgta atgtggacat aacgatttcc
    19381 tttcttgtag taatgggtca ctcttttttc tcgctttctt cctcttccgg caccggttta
    19441 ctggcggtta acaggaaggg cgattgctgc cagcgggtgc gtttaccctg tagcagcgtg
    19501 cgggtcagca ctacgccgat tgccagtgag agcagcaaca tcaggcgtaa aatgttagtg
    19561 gtgttatcca cctgttgggc ttcggtggcg agcgtatggg tgtcgagcgt aaggcgcaga
    19621 tagccgagcg gtccgttttt cccggcaatt ggttcgacaa tctgctggtt aaaatagccg
    19681 cccgcttttt tgccgtcgag cgccaaccgg tcgcgcacgt cgacgctttc gccagaacgg
    19741 gcgataagat cgccttgttc gtcatacacg cccgcgtcga ggatacggct ttcatccgtt
    19801 aactgatcaa ggatcgcctg aatgcgtttt tcatccggtg agtcggtacg catcagcggt
    19861 gcaacgttca gcgtcacctg acgcgccagg gtgcgggcca gttcttccag ctgtggatta
    19921 cgctgtcgct ggtggttttg actaaaccat gacgctccct gcatcagcgc cactaacaag
    19981 gcaagacaga acaggacaat cactgcccga tgcagccgga atttcagttt tgtgcgagcc
    20041 atcttccacc ctttgaaaat ttgagactta atgttgccag aagcaatgga tacaaggtag
    20101 cctcatgcgt tattttccct gcttcgaacg attttacagg agccttaatg cctaacatta
    20161 cctggtgcga cctgcctgaa gatgtctctt tatggccggg tctgcctctt tcattaagtg
    20221 gtgatgaagt gatgccactg gattaccacg caggtcgtag cggctggctg ctgtatggtc
    20281 gtgggctgga taaacaacgt ctgacccaat accagagcaa actgggcgcg gcgatggtga
    20341 ttgttgccgc ctggtgcgtg gaagattatc aggtgattcg tctggcaggt tcactcaccg
    20401 cgcgggctac gcgcctggcc cacgaagcgc agctggatgt cgcgccgctg gggaaaatcc
    20461 cgcacctgcg cacgccgggt ttgctggtga tggacatgga ttccaccgcc atccagattg
    20521 aatgtattga tgaaattgcc aaactggccg gaacgggcga gagggtggcg gaggtaaccg
    20581 aacgggcgat gcgcggcgaa ctcgatttta ccgccagcct gcgcagccgc gtggcgacgc
    20641 tgaaaggcgc tgacgccaat attctgcaac aggtgcgtga aaatctgccg ctgatgccag
    20701 gcttaacgca gctggtactc aagctggaaa cgctgggctg gaaagtggcg attgcctccg
    20761 gcggctttac tttctttgct gaatacctgc gcgacaagct gcgcctgaca gcagtggtag
    20821 ccaatgaact ggagatcatg gacggtaaat ttaccggcaa tgtgatcggc gacatcgttg
    20881 acgcgcaata taaagcgaaa accctgaccc gcctggcgca ggagtatgaa atcccgctgg
    20941 cgcagaccgt ggcgattggc gatggagcca atgacctgcc gatgatcaaa gcggcagggc
    21001 tggggattgc ctaccatgcc aagccaaaag tgaatgaaaa gacggaagtc accatccgtc
    21061 acgctgacct gatgggggta ttctgcatcc tctctggcag cctgaatcag aagtaattgc
    21121 ctgcccgtca tcctgcgggc ggcacagcat taacgaggta caccgtggca aaagctccaa
    21181 aacgcgcctt tgtttgtaat gaatgcgggg ccgattatcc gcgctggcag gggcagtgca
    21241 gcgcctgtca tgcctggaac accatcaccg aggtgcgtct tgctgcgtcg ccaacggtgg
    21301 cgcgtaacga gcgtctcagt ggctatgccg gtagcgccgg agtggcaaaa gtccagaaac
    21361 tttccgacat cagccttgag gagctgccgc gtttttccac cggatttaaa gagttcgacc
    21421 gcgtattagg cggcggggtg gtgccgggaa gcgccattct gattggcggt aacccaggtg
    21481 cggggaaatc cacgctgttg ctgcaaacgc tgtgcaaact ggcgcagcag atgaaaacgc
    21541 tgtatgtcac tggcgaagag tcgctgcaac aggtggcaat gcgcgctcat cgccttggcc
    21601 tgccgactga caatcttaat atgctgtcgg aaaccagcat cgagcagatc tgcctgattg
    21661 ccgaagaaga gcaaccgaag ctgatggtaa ttgactccat ccaggtgatg catatggcgg
    21721 atgttcagtc atcgcctggc agcgtggcgc aggtgcgtga aacggcggct tatctgacgc
    21781 gcttcgccaa aacgcgcggc gtggcgattg tcatggttgg gcacgtaacc aaagatggct
    21841 cgctggctgg ccctaaagtg ctggaacact gtatcgactg ttcggtgctt ctggatggcg
    21901 atgccgactc tcgttttcgc accttacgca gccataaaaa ccgcttcggc gcggtgaatg
    21961 agctgggcgt cttcgcgatg accgaacagg ggctgcgtga agttagcaac ccttccgcca
    22021 tctttttaag ccgcggtgat gaagtgacct ccggtagctc agtgatggta gtgtgggaag
    22081 gaacgcgtcc actgttggtg gagatccagg cgctggtcga tcactcgatg atggcgaacc
    22141 cacgccgcgt cgcagtaggg ctggagcaaa accgtctggc aatcctgctg gcggtgctgc
    22201 accgtcacgg tgggttgcaa atggccgatc aggatgtatt tgttaacgtg gtcggcggcg
    22261 tgaaggtaac ggaaaccagc gccgacttag cgttactgct ggcgatggtt tccagcctgc
    22321 gcgacagacc gctgccgcag gatctggtgg tgtttggtga agtcgggctg gcaggggaga
    22381 tccgcccggt gcccagcggc caggaacgaa tttctgaagc ggcgaaacac ggttttcgcc
    22441 gggcgattgt tccggcggct aacgtgccga aaaaagcgcc ggaagggatg cagatttttg
    22501 gcgttaaaaa actctccgac gcgcttagcg tgttcgacga cttataatga gagatacgga
    22561 gggagatatg tcgtcatttg attacctgaa aactgccatc aagcaacagg gctgcacgct
    22621 acagcaggtc gctgatgcca gcggtatgac caaagggtat ttaagccagt tactgaatgc
    22681 caaaattaaa agccccagcg cgcaaaagct ggaggcgttg caccgttttt tggggcttga
    22741 atttccccga cagaagaaaa ccattggtgt cgtattcggt aagttctacc cgctgcatac
    22801 cggacatatc taccttatcc agcgcgcctg tagccaggtt gacgagctgc atatcattat
    22861 gggctttgac gatacccgcg accgcgcgtt gttcgaagac agcgccatgt cgcagcagcc
    22921 cacggtgccg gatcgtctgc gctggttatt gcaaaccttt aaatatcaga aaaatatccg
    22981 tattcatgct ttcaatgaag agggcatgga gccatatccg cacggctggg atgtatggag
    23041 caacggcatc aaaaagttta tggctgaaaa ggggatccag ccggacctga tctacacctc
    23101 ggaagaagcc gatgcgccac agtatatgga acatctgggg atcgaaacgg tgctggtcga
    23161 tccgaaacgc acctttatga gtatcagcgg tgcgcagatc cgcgaaaacc cgttccgcta
    23221 ctgggaatat atccccactg aagtgaagcc gttttttgtg cgtaccgtgg cgatccttgg
    23281 cggcgagtcg agcggtaaat ccaccctggt aaacaaactt gccaatatct tcaataccac
    23341 cagtgcgtgg gaatatggtc gcgattatgt cttttcacac ctcggcggtg atgagatcgc
    23401 attgcagtat tccgactacg ataaaatcgc gctgggacac gcacaataca ttgattttgc
    23461 agtgaaatat gccaataaag tggcgtttat cgacactgat tttgtcacta cccaggcgtt
    23521 ctgcaaaaag tacgaagggc gggagcatcc gttcgtgcag gcgctgatcg atgaataccg
    23581 ttttgatctg gttatcttgc tggagaacaa caccccttgg gtggcggatg gtttacgcag
    23641 cctcggcagt tcggtggatc gcaaagagtt ccagaacttg ctggtggaga tgctggaaga
    23701 gaacaatatc gaatttgtgc gggttgaaga agacgattat gacagccgtt tcctgcgctg
    23761 cgtggagctg gtgcgggaga tgatggggga gcaaagataa tcatctttat aagatgttga
    23821 gtgggattac tgattcaaaa gtgtacttag tactccattt tggtgtacaa tgtaccttcc
    23881 agggaggggt aatgggtaag gcgataatat ttatagagac gccaatgttt acgcgtcaga
    23941 tcaagcagat tgcaaccgat gatgaattaa aggaattaca aaaagaactc atcggaactc
    24001 ccgataaagg tgatctcatt cagcagactg gtggattgcg taaagtccga atggctgcgg
    24061 gttcacaggg aaaaagtggc agtgtcagaa taatttactt tctggcgacg gaagagatta
    24121 tttatttgat tatggcctat ccgaaaaatg ccaaagatag tctgacagat acggaaaaag
    24181 ctcaactaaa gaagttgacg aaactgctca aaggcgagat ataaaatgag tttttttgat
    24241 gagttgaaaa cctctctgga agaggctgtc gagattaaac aaggtttaaa agaacctgca
    24301 cgggtgaccc gctacgaaat tgcggatgtt aaagctattc gtgagcaact caacgtttcg
    24361 caaagtgaga tggcaaaagc tctgggaacc agtgtggata ccattaaaag ctgggaatcg
    24421 aagcgaagaa atcctacggg attggctgca aaagtacttg ccacaattaa agaaaatcct
    24481 gactttttcc gggaacttgc ctcccattaa tcatttaact aataccggat gcgatgctgg
    24541 cgcgccgcat ccggcatttt acgcattact tcgcaatacg cttgtacttg atacgcttcg
    24601 gctccagcgc gtctgcgccc agcgtgcgtt tcttgtactc ttcgtactcg gtaaagttac
    24661 cttcgaagaa ctcaacttta ccctcatcct ggtaatccag gatgtgcgtg gcgatacggt
    24721 cgaggaacca acggtcgtgc gagataacca tcgcgcagcc cgggaactcc agcagggcgt
    24781 tttccagcgc gcgcagggtt tcgatatcca ggtcgttggt tggttcgtcg agcagcagca
    24841 tgttgccgcc aacttgcagc aacttcgcca gatgcagacg accgcgctca ccgccggaga
    24901 gttcgccaac gcgtttaccc tgatcaaccc ctttaaagtt aaagcggcca acgtaggcgc
    24961 ggcttggcat ctcggtgttg ccgattttca tgatatccag cccgccggaa acttcttccc
    25021 aaacggtttt gctgttatcc attgagtcac ggaactgatc aaccgatgcc agtttcaccg
    25081 tttcacccaa agtgatggtg ccgctgtccg gctgttcctg accagagatc atacggaaca
    25141 gcgtcgattt acccgcaccg ttcggaccga tgatcccgac gatcgcccct ttcgggatcg
    25201 agaaactcag ggagtcaatc agcagacgat cgccatagga tttacgcaga ttgctgactt
    25261 ccagcacttt gtcgcccaga cgcggtccag gtggaataaa cagttcgttg gtttcgttac
    25321 gtttctgata ttcggtgctg ttcagttctt caaagcgcgc cagacgtgct ttacctttcg
    25381 actgacggcc tttagtacct tgacgtaccc actccagctc tttctcaatc gacttacgac
    25441 gcgcagcttc ttgtgaagct tcctgcgcca gacgctgatc tttctgctcc agccaggagg
    25501 agtagttacc ttcccacgga ataccttcac cgcggtccag ttcgaggatc cagcccgcaa
    25561 cgttatcgag gaagtaacgg tcgtgggtaa tcgccacaac ggtgccttcg aagtcgtgca
    25621 ggaagcgttc cagccaggcc acggattcgg catccaggtg gttggtcggt tcgtcgagca
    25681 gcagcatgtc tggtttttcc agcagcaggc ggcacaacgc cacgcgacga cgctcaccac
    25741 cggagaggtt agcgattttc gcgtcccaat ccggcagacg cagcgcatcc gccgcacgct
    25801 ccagctgaac gttcaggttg tgaccgtcgt gagcctgaat gatctcttcc agacggccct
    25861 gttcagcggc cagtttgtca aaatcggcat ccggatcggc gtacagtgca tacacttcat
    25921 ccaggcgttt cagggcgtta accacttcag aaaccgcttc ttcaatggac tcacgcacgg
    25981 tgtgttccgg gttcaactgc ggttcctgcg gcagataacc aatcttgata tccggttgcg
    26041 gacgcgcttc gccttcgatg tctttatcaa tgcccgccat aatgcgcagc agggtggact
    26101 tacccgcgcc gttcagaccc aggacaccaa tttttgcccc agggaagaaa ctcagagaga
    26161 tgtttttcaa aatatgacgt ttcggcggaa caactttgcc gacacgatgc atggtataaa
    26221 cgaattgagc cacgttggac ttcgcctcta tgtttatcgt gatgatgagt tttcaaaggc
    26281 gaagtgtagc ctgtttcctt atctaatccc agccggtcga tcaccctcgc agtatactcg
    26341 tcatacttca agttgcatgt gctgcgctta cgctcgctca ccccagtcac ttacttctgt
    26401 aagctcctgg ggactcactc acttatcgcc ttcctgcaac tcgaattatt tagagtataa
    26461 aagtaaaaaa gtgtccgtaa cgtggcgtaa acggcaatga ctggttagca taaatctatt
    26521 acgcggcatg acgctgcatt gatgtattta cacttagagg atgcgcttgt ggaaaaagcc
    26581 aaacaagtta cctggcggct gttggctgcc ggtgtctgtc tgctgacggt cagcagcgtg
    26641 gcgcgagccg actcactgga tgagcagcgt agccgctacg cccaaatcaa gcaggcctgg
    26701 gataatcgac aaatggatgt ggtcgaacaa atgatgcctg gactgaaaga ttatccgctt
    26761 tatccctacc tggaataccg tcagatcacc gatgacctga tgaatcaacc ggcggtgacg
    26821 gtcactaact ttgttcgcgc taaccccacg cttcctcccg ctcgcacgct gcaatctcgt
    26881 ttcgtcaatg aactggcgcg gcgtgaagac tggcgtggct tgttagcctt tagcccggaa
    26941 aagcccggaa ctaccgaagc gcaatgtaat tactactatg cgaaatggaa caccgggcag
    27001 agtgaagaag cctggcaagg ggcgaaagag ctgtggctaa ccggcaagag ccagcctaac
    27061 gcctgtgaca agttatttag cgtctggcgt gcgtcaggta aacaagatcc gctggcgtat
    27121 ttagagcgta tccgtctggc gatgaaagcg ggtaacactg gactcgtaac agtgctggca
    27181 gggcagatgc ctgccgatta ccagactatc gcctcggcaa tcatttcact ggcgaacaac
    27241 cctaatacgg tactgacctt cgcgcgtaca accggagcga ccgattttac ccgtcaaatg
    27301 gcggcggtgg cgtttgccag tgtggcgcgg caggatgctg aaaatgcacg gctgatgatc
    27361 ccgtcgcttg cccaggcgca gcagcttaat gaagatcaga ttcaggagct gcgcgatatc
    27421 gtcgcctggc gtttgatggg caatgatgtc accgatgagc aggcgaaatg gcgcgatgac
    27481 gccattatgc gttcgcaatc tacttcgctt attgaacggc gcgtgcgaat ggcgcttggc
    27541 accggcgatc gtcgcggcct gaatacctgg ctggcgcgtt tgccgatgga ggcgaaagag
    27601 aaagatgaat ggcgttactg gcaggcggat ttactgctgg aacgcggacg tgaagctgaa
    27661 gcaaaagaga ttttgcatca actcatgcaa cagcgtggtt tctacccgat ggttgcagca
    27721 caacgcatcg gtgaagagta tgagctgaag attgataaag cgccgcagaa tgttgacagc
    27781 gccctgactc aggggccgga gatggcgcgc gtgcgcgagt tgatgtactg gaatctcgat
    27841 aataccgcgc gtagcgagtg ggccaatctg gtgaagagca agtcaaaaac agagcaggcg
    27901 caactggctc ggtatgcttt caataaccaa tggtgggaac ttagcgttca ggcaacgatc
    27961 gccgggaagc tttgggatca tctggaagag agattcccgc tggcctacaa cgatcttttc
    28021 aaacgttaca ccagtgggaa ggagatcccg caaagctatg cgatggcgat tgcccgtcag
    28081 gagagcgcct ggaatccgaa agtgaaatca ccggtagggg ccagcggcct gatgcagatt
    28141 atgccgggca cggcgaccca tacggtgaag atgttctcta ttcctggtta tagcagccct
    28201 gggcaattgc tggatccgga aacgaacatc aacattggca ccagttacct gcaatatgtt
    28261 tatcagcagt ttggcaataa ccgtattttc tcctcagcag cttataacgc cggacctgga
    28321 cgggtgcgaa cctggcttgg caacagcgcc gggcgtatcg acgctgtggc atttgtcgag
    28381 agtattccgt tctccgagac gcgcggttat gtgaagaacg tgctggctta tgacgcttac
    28441 taccgctatt tcatggggga taaaccgacg ttgatgagcg ccacggaatg gggacgtcgt
    28501 tactgatccg cacgtttatg atatgctatc gtactcttta gcgagtacaa ccgggggagg
    28561 cattttgctt cccccgctaa caatggcgac atattatggc ccaacaatca ccctattcag
    28621 cagcgatggc agaacagcgt caccaggagt ggttacgttt tgtcgacctg cttaagaatg
    28681 cctaccaaaa cgatctccat ttaccgttgt taaacctgat gctgacgcca gatgagcgcg
    28741 aagcgttggg gactcgcgtg cgtattgtcg aagagctgtt gcgcggcgaa atgagccagc
    28801 gtgagttaaa aaatgaactc ggcgcgggca tcgcgacgat tacgcgtgga tctaacagcc
    28861 tgaaagccgc gcccgtggag ctgcgccagt ggctggaaga ggtgttgctg aaagatcact
    28921 gatacaccgc attatgaaac ggactcagcg ccaggatcac cgcctggtga tagacgctgg
    28981 cgcgggtaag tttcccggcg gtaaacacgc cgatcgcccc ttccttacga ccaatctcat
    29041 caataccggt ataacgtgac atcacgggac cgagcgcctc gccttcacgc actttttcca
    29101 gaatcaccgc aggtaacggc aaagttgccg aacgcgcctc gccgcgctgg ctggcgtttt
    29161 caatcaccac ccaactgaag gtgctgtcgc catcaatgcc tgcttcaatc gccacccaaa
    29221 aatcagcctc tggatgtaaa cggcgggcat ttgctacccg atttcgtgcg ccagcgcgcg
    29281 tttcctcact gccaaagggt tgttccggta caccgctctc gacggcaacg gatgcaatat
    29341 ggcaggatcc ttcgccgaag atctcgtgaa atgcctgcag aatggcctga attttagcgg
    29401 gattggtggt cgcacagaca acttggtgca taatcagcat tactcagaaa attaacgtta
    29461 cagcagtata cggaaaaaaa gcatgttaca ggtataccta gtccgccacg gtgaaacgca
    29521 gtggaacgcc gagcgacgta ttcagggcca gtctgacagc ccgctgaccg ccaaaggtga
    29581 gcaacaggcg atgcaggtgg caacccgtgc caaagagctt ggcattacgc atatcatcag
    29641 tagcgattta ggacggaccc ggcgcacggc ggaaatcatc gcccaggcct gcggctgtga
    29701 tatcatcttt gattctcgcc tgcgtgaatt aaatatgggt gtactggaaa caagaaatat
    29761 cgattcactc accgaagaag aagagaactg gcgtcggcag ctggtcaatg gcaccgttga
    29821 cgggcgtatt cctgaaggcg agtcaatgca ggagctcagc gatcgcgtca atgcggcact
    29881 ggaatcttgc cgggacttac cgcagggaag ccgaccgctg ctggtgagcc atggtattgc
    29941 actgggatgt ctggtgagta cgattctcgg attaccagca tgggcagagc gccgcttacg
    30001 tctgcgtaac tgttctattt cgcgcgtgga ttatcaggaa agcctgtggc tggcgtctgg
    30061 ctgggtcgtt gaaaccgcag gggacatctc gcatctggac gcccctgcat tagatgagct
    30121 gcagcgttaa cgacggatcg gaatcagtag ttcacagcgc agattaattg ggcgatctcc
    30181 cgctttggca tcttctgccg ggtagtatcg ctcaatatcc tgacctttac ggcgcgtcag
    30241 gttgagcatt ggcatgcacg tcccgtatac cgtcaggata aactcctgca cgccggttcc
    30301 cagaccttca taggtaaaca tcacatattc gccgccctgc agcatcaccg gatgccccgt
    30361 cagtacatag ccatctgcct gatcctgggc taacgcggtg gtatagaata cttcttgttc
    30421 gtcgtcttta tcctgactcg gacgcgtttc attcaggccg tagagcaccg gcggaatggt
    30481 cggcgcgttg ccgagaaaat cgtgccagaa ctgataacgc atttcatggc ggaaatcaga
    30541 gatttgctcc agcgaacagg agtagctctg ggtaacacca atcagcggcg tatcttccag
    30601 ggtgacaaat ttgtgctctg gcatagtgaa ttcacccaga cgtagcggcg ggcgaatacc
    30661 aaaggcgctc cattcaggag aacggcggta aagtgcagga gtctgggcaa actgcttctt
    30721 gaatgcgcgg gtaaatgtct gttgagagtc gaagcggtat tgcagcgcga tgtccagaat
    30781 cggacgcgca gtcaggcgta gtgcgaccgc cgatttcgac aaacgacgag cacgaatata
    30841 cgcgccaata gcatggccag tgacatcttt aaacattctc tgtaagtgcc acttggaata
    30901 acctgctttc gccgctacat tgtcgagcga caggggctga tccagatgac cttccagcca
    30961 gattaaaagg tcgcgaataa tgccggcctg atccataaaa tatcctcatc ctttcaacaa
    31021 cgagcacctg acatcaggta attggataat a
//
LOCUS       DAZGAP010000036        30715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGAP010000036 DAZGAP010000000
VERSION     DAZGAP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.036"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /gene="radC"
                     /locus_tag="ACHLAM_001399"
     CDS             <1..354
                     /gene="radC"
                     /locus_tag="ACHLAM_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7852957.1"
                     /translation="AAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTE
                     VHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLI
                     VGGSQVFSFAEHGLL"
     gene            423..644
                     /locus_tag="ACHLAM_001400"
     CDS             423..644
                     /locus_tag="ACHLAM_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7852958.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            720..1073
                     /locus_tag="ACHLAM_001401"
     CDS             720..1073
                     /locus_tag="ACHLAM_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7852959.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAG
                     IRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLGSC
                     GYVYMAVYPTPETKK"
     gene            1163..1537
                     /locus_tag="ACHLAM_001402"
     CDS             1163..1537
                     /locus_tag="ACHLAM_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7852960.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            1534..2022
                     /locus_tag="ACHLAM_001403"
     CDS             1534..2022
                     /locus_tag="ACHLAM_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7852961.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPTF
                     DW"
     gene            2034..2231
                     /locus_tag="ACHLAM_001404"
     CDS             2034..2231
                     /locus_tag="ACHLAM_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7852962.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            2316..3158
                     /locus_tag="ACHLAM_001405"
     CDS             2316..3158
                     /locus_tag="ACHLAM_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7852963.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITCNLDREIWRDLMQCS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDQWGLHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSLQGKECYEDEMFIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(3308..3395)
                     /locus_tag="ACHLAM_001406"
     tRNA            complement(3308..3395)
                     /locus_tag="ACHLAM_001406"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3359..3361),aa:Ser,seq:gga)
     gene            complement(3649..3867)
                     /gene="infA"
                     /locus_tag="ACHLAM_001407"
     CDS             complement(3649..3867)
                     /gene="infA"
                     /locus_tag="ACHLAM_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO7852964.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(4152..4856)
                     /gene="aat"
                     /locus_tag="ACHLAM_001408"
     CDS             complement(4152..4856)
                     /gene="aat"
                     /locus_tag="ACHLAM_001408"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO7852965.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(4898..6619)
                     /gene="cydC"
                     /locus_tag="ACHLAM_001409"
     CDS             complement(4898..6619)
                     /gene="cydC"
                     /locus_tag="ACHLAM_001409"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO7852966.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(6620..8386)
                     /gene="cydD"
                     /locus_tag="ACHLAM_001410"
     CDS             complement(6620..8386)
                     /gene="cydD"
                     /locus_tag="ACHLAM_001410"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO7852967.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(8509..9474)
                     /gene="trxB"
                     /locus_tag="ACHLAM_001411"
     CDS             complement(8509..9474)
                     /gene="trxB"
                     /locus_tag="ACHLAM_001411"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO7852968.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10018..10512
                     /gene="lrp"
                     /locus_tag="ACHLAM_001412"
     CDS             10018..10512
                     /gene="lrp"
                     /locus_tag="ACHLAM_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO7852969.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            10647..14714
                     /gene="ftsK"
                     /locus_tag="ACHLAM_001413"
     CDS             10647..14714
                     /gene="ftsK"
                     /locus_tag="ACHLAM_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO7852970.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQTAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQHPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAHHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            14873..15484
                     /gene="lolA"
                     /locus_tag="ACHLAM_001414"
     CDS             14873..15484
                     /gene="lolA"
                     /locus_tag="ACHLAM_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO7852971.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSNFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            15495..16838
                     /gene="rarA"
                     /locus_tag="ACHLAM_001415"
                     /gene_synonym="mgsA"
     CDS             15495..16838
                     /gene="rarA"
                     /locus_tag="ACHLAM_001415"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO7852972.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLSCAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            16929..18221
                     /gene="serS"
                     /locus_tag="ACHLAM_001416"
     CDS             16929..18221
                     /gene="serS"
                     /locus_tag="ACHLAM_001416"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO7852973.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            18460..20904
                     /gene="dmsA"
                     /locus_tag="ACHLAM_001417"
     CDS             18460..20904
                     /gene="dmsA"
                     /locus_tag="ACHLAM_001417"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO7852974.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            20915..21532
                     /gene="dmsB"
                     /locus_tag="ACHLAM_001418"
     CDS             20915..21532
                     /gene="dmsB"
                     /locus_tag="ACHLAM_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO7852975.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            21534..22397
                     /gene="dmsC"
                     /locus_tag="ACHLAM_001419"
     CDS             21534..22397
                     /gene="dmsC"
                     /locus_tag="ACHLAM_001419"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO7852976.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(22433..23059)
                     /gene="ycaC"
                     /locus_tag="ACHLAM_001420"
     CDS             complement(22433..23059)
                     /gene="ycaC"
                     /locus_tag="ACHLAM_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO7852977.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            23374..24522
                     /gene="ycaD"
                     /locus_tag="ACHLAM_001421"
     CDS             23374..24522
                     /gene="ycaD"
                     /locus_tag="ACHLAM_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7852978.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            24732..26162
                     /gene="ycaM"
                     /locus_tag="ACHLAM_001422"
     CDS             24732..26162
                     /gene="ycaM"
                     /locus_tag="ACHLAM_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7852979.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(26163..26273)
                     /locus_tag="ACHLAM_001423"
                     /pseudo
     CDS             complement(26163..26273)
                     /locus_tag="ACHLAM_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(26372..27112)
                     /gene="pflA"
                     /locus_tag="ACHLAM_001424"
     CDS             complement(26372..27112)
                     /gene="pflA"
                     /locus_tag="ACHLAM_001424"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO7852980.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMREVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(27304..29586)
                     /gene="pflB"
                     /locus_tag="ACHLAM_001425"
     CDS             complement(27304..29586)
                     /gene="pflB"
                     /locus_tag="ACHLAM_001425"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7852981.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(29641..30498)
                     /gene="focA"
                     /locus_tag="ACHLAM_001426"
     CDS             complement(29641..30498)
                     /gene="focA"
                     /locus_tag="ACHLAM_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO7852982.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         7592 a         7625 c         8021 g         7477 t
ORIGIN      
        1 gcggcacggg aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg
       61 ctgtatctga acaaccagaa tcagttgatt gccggtgaaa ccctcttcac cggcaccatc
      121 aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg ccctgtacca caatgccgct
      181 gccgtggtac tggcacacaa tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgg
      241 cttattacgg aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat
      301 ctgatagtcg gtggcagcca ggttttctcc tttgccgaac acggtctgct ttaacccgtc
      361 accgtcacaa ccacatcata cctgttttca cttttatctt ctgtcttcag aggtatcaca
      421 tcatgaaaat catcacccgt ggtgaagcca tgcgtattca ccaacagcat ccggcctccc
      481 gtctttttcc gttctgcacc ggtaagtacc gctggcacgg cagtgccggg gcgtataccg
      541 gtcgtgaagt acaggatatt cccggtgtgc tggccgtgtt tgctgaacgc cgtaaggaca
      601 gttttggccc gtatgtccgg ctgatgagcg tcaccctgaa ctgagtcagg gcattctgat
      661 gagcagaatt atcgccacca ccggaccatt cttaaccgat tttctgtgag gattttatcg
      721 tgtcagacac acttcccgac ggcaatcacg accgcccctg gtgggggctg ccctgcaccg
      781 tgacgccctg tttcggcgcc cgtctggtgc aggagggtaa ccggttgcat taccttgccg
      841 accgcgctgg tatcagaggc ctgttcagcg atgcagatgc gtaccacctg gaccaggcct
      901 ttccgctgct gatgaaacaa ctggaactca tgctcaccag cggtgaactg aatccccgcc
      961 atcagcatac cgtcacgctg tatgccagag ggctgacctg cgaagccgac accctcggca
     1021 gttgtggcta cgtttatatg gctgtttatc cgacgcccga aacgaaaaag taactctcca
     1081 gaataaccct ctgctccggc ctggtgtttt caccacacca ctttcccatt tttcatcttt
     1141 gcatatcagg aaaatcttca gtatgaaaac attacctgta ttacccgggc agtcggccag
     1201 ttcacgccca tctcctgttg aaatctggca gacactgctc acccgactgc tggaccagca
     1261 ctatggcctt acgctgaatg acacaccgtt cgctgatgaa cgtgtgattg agcagcatat
     1321 tgaggcaggc atttcactgt gtgatgcggt gaattttctc gtggaaaaat acgcgctggt
     1381 gcgtaccgac cagccgggat tcagcgcagg aacttcgtcg cagttaatca acagcattga
     1441 tattctccgg gcacgccggg caacaggtct gatgacccgc gacaattaca gaacggtaaa
     1501 taacattacc ctgggtaagc atccggagaa acgatgaaac tttccctgat gctggaagcc
     1561 gacagaatta atgtgcaggc actgaacatg gggcaaattg tcgttgacgt cgatggtgtt
     1621 aatctcactg aactgattaa caaggtcgct gaaaacggtt attcactccg cgtggtggag
     1681 gaatccgacc aacagtcaac ctgcacacta ccaccgtttg caacccttgc cggcatacgc
     1741 tgcagtaccg cacatatcac ggaaaaggat aacgcctggc tgtactcgct gtcgcatcag
     1801 accagtgacg ttggtgaatc agaatggatt catttcacag gtagcggata tctgttacgt
     1861 accgatgcgt ggtcataccc ggttctgcgg cttaaacgcc tggggctgtc aaaaacgttc
     1921 cgccgtctgg ttgtcacact catccgacgt tatggcgtca gtctcattca tctggatgcc
     1981 agtgccggat gtctgccggg tttccccact ttcgactggt aaccagaaac aacatgaaat
     2041 cattaaccac ggaaacagcg ctggatattc tgattgcatg gctgcaggac aatatcgact
     2101 gcgaatcggg aattatcttt gacaacgatg aggataaaac ggattcggca gcactgctgc
     2161 cctgcattga acaggccagg gaagatatcc gtaccctgcg ccaactgcag tttctgcaac
     2221 agaaccggtg agtcttactc accagacttc attaccctga cgtcagcctg aacagggctg
     2281 gcgttttcgt ttataaaccc aaagggaaac cgataatgtc agacatcaca atctcccgtc
     2341 cggaactggt caccgggcat acggacgtta tctgctcaac cagtatccgc cacattctgg
     2401 ctgtacgaaa gagtacactg ctgcaaatcg acacacttat ccggcaactg gctgaaatat
     2461 cagcaatgac agaaagtatt ggcggtaaaa ccgcactgga ctgggcaatg aaacaggatt
     2521 ttcgctgcgg ttgctggctg atggaaaaac ctgaaacggc aatgaaagcc atcacctgca
     2581 atctcgatcg agaaatctgg cgtgacctga tgcaatgctc cgggatgctt tccttaatgg
     2641 atgcacaggc ccgtgataca tggtaccggt cactggagta cgataatttt ccggaaatca
     2701 gtgaagagaa tattctgagt acgtttgagc agctacacca gaataaggat gaagtgtttg
     2761 agcgaggagt gatcaacgtc ttcagagggt tgagctggaa ttacaaaacc aattgcccct
     2821 gtaaatttgg caataaaatt atcgtcaaca atctggtgag gtgggaccag tggggattgc
     2881 atcttatcac cgggcaacag gcagatagac ttgccgacct ggaaagaatg ctgcatctgt
     2941 tcagcggcaa accgatcccc gacaacaggg aaaacataac tattcatctg gacgatcaca
     3001 tccggtctct tcagggaaaa gaatgctatg aagatgagat gttcattatt aaatacttta
     3061 agaagggctc tgcgcacatc acgttcagga agccagaact ggttgacaga ctgaatgata
     3121 ttatcgcaaa acactatcct gacatgctgg cagtatagct ttgccagtat gctcaaagat
     3181 cggaaattgt ccgataagat atgatcgtgg ggcatataaa ccggtttcag atggcatacc
     3241 cggtggcata ctgtctggaa ggataaataa aaaagcgcat gttaaacaat aacatgcgct
     3301 tgtaatctgg cggtgagggg gggattcgaa cccccgatac gttgccgtat acacactttc
     3361 caggcgtgct ccttcagcca ctcggacacc tcaccaaatt gttttgctac caaacctcat
     3421 gggtggcaac ggggcgctac tatagggagt tggagtaaaa cggtcaagaa gaattttaat
     3481 gataattatt gtttgctcat actgtaaaca agttgtgcag tatatctaca tcgagacaag
     3541 ttatggactt atacttccaa agtacttcat acatatcaca aaataaaaag gccggctaaa
     3601 ccgacctttt actcgttctt tctcttcgcc catcaggcgg taaaacaatc agcgactacg
     3661 gaagacaatg cggcctttgc tcaggtcgta cggggtcagt tcaacagtca ctttgtcgcc
     3721 cgtcaggatg cggatgtagt ttttgcgcat tttaccggag atgtgtgcag taaccacgtg
     3781 accgttttct aactctacgc ggaacatggt attaggcaac gtttcaagaa cggtaccttg
     3841 catttcaata ttgtcttctt tggccatcta atcctctggg gtatcactac cgtaatttga
     3901 accggcaaga taatgccgaa gttctgtaaa taagtaaaga tttgcgcgct aaatcgcaac
     3961 aaacaggttc ggcacattac tccgaaaaca cacggctaag ccgcaccaaa agcgcaacgt
     4021 ataagggagc ggtgagataa acgatgggcg ttacctgacg cgaaaaattc cttatcggca
     4081 gcggggtaat gagcgtaacc aactctgcga ccgcaattat aacactctgg ggagaaatgt
     4141 gccgaaaaca ttcattcttg tggtgaaaac aagcaccgtg gtacccagaa attattcggc
     4201 aatcgtccga ggcgcatttg attgagataa ttgaggtaat cccggcgggg aatttcgcag
     4261 gcaccaagcg acgctgtgtg atcgttaagg acctggcagt cgataagttt accgccatga
     4321 ccaataaatt cctcacagaa taccagaagc gccgttttag acgcattttc catccggctg
     4381 aacatggact cgccacaaaa tagcgttccc tgggccacgc cgtacatacc gccgacaagc
     4441 tcatcttcac gccagacttc aatagagtgg gcatgaccga gttcgtgaag gcgatggtag
     4501 gcttcgacca cgccacgcgt gatccaggtt ccttcttcgc gatcgctggc acagccttca
     4561 atgacctgac caaaagcgta attcatcgtg actcgatagg gcgagcgttt atgaaatcgc
     4621 ttcatactac ggctgatatg cagtgattca ggccaaagca ccgcgcgggg atcgggcgac
     4681 caccagagga tggggtcacc tggagaaaac cacggaaaaa taccacgctg gtaagccatt
     4741 aacaggcgcg cagggctaag atcgcctccc agtgccagca ggccgttagg ctcacgtaat
     4801 gcgccttccg gggaagggaa ggctattgaa tggcgagaaa gctgaaccag gcgcatgacc
     4861 gcaaaactcc acgcaagtcg gatcgttcaa taatagctta caaaccctgc ttaaactggt
     4921 aataacgccc ctgtctggca agcagttctg cgtgagtacc ttgctcaata atttgcccgt
     4981 tgtccatcac tattatttgt tggaaacgag agagtccgcg aagtcgatgg gtgaccatta
     5041 acaccgtttt ctcatgcatc atttctgcaa gcaattcaag gatctgactt tcggttgtgg
     5101 catctaagcc ttctgtaggt tcatccagca ataccagcgg cgcattatgt aacagcgcac
     5161 gggcgatagc cagacggcgt agttcaccac cggagagctg gcgtccgcct tcacccaacc
     5221 aactgttgag acctgcatcc tcgagcagct tctccagacc aacgcgacgt aagatctccg
     5281 ccagagcctc atcactactg ccaggcgagg cgagtaacag attatcacgc agcgtggcgc
     5341 taaacagatg cactcgctga ggaacaacgc tgatggtctg tcgtagagtc gcttcattca
     5401 ggctggctat ggggctaccg ttaagcaaaa tctcgccctg ttgcgggtcc catgcgcggg
     5461 tcagcagttg taacagtgtt gatttgccgc atccggttcg cccgagaatc gctatatgtt
     5521 ccccggcgtt tacctgaaga gaaatccctt taagtgcctg ttgggattgc tccggataat
     5581 tgaattgaac atcccgtaac gtcagcgaaa cgcgatcggc aacacgagtt tgggtatcag
     5641 ggaaggtgac ttccggtttt tgatccgtta agtcagtgat ccgtacggca gaggcaatga
     5701 cttgccccag atgctgaaat gcacccgtta ctggtgccag tgcttcaaac gcggctaacg
     5761 cgcagaagac aaacagggca attaacgcgc cgggttgagc attgccgcca acgccgccag
     5821 acgccatcca cagcatcagg atcaccgcta acgcgccaat gagcagcatt atcgcttgcg
     5881 acaatgcggt gagttcagat tgacgacgtt gcgcttccag ccactgaatt tctgtattct
     5941 ctaattgcgt gcgataacga tcgctggcac caaagatggt cagctcagct tgcccttgca
     6001 gccaggccgt cagttgttgg cgatactgcc cgcgaagatg agtcagattt tgcccggtgc
     6061 tttttcccgc acgataaaac agtggtggca taaggaaaag cgtcagtaac ataatgccgc
     6121 ccagcgtaaa ggcgagggtg aaatcaagga aacttaaccc gattgtcacc accataatca
     6181 ccacaaaagc gcccaccagc ggcgagataa cgcgcaggta aagatgatcg agcgtatcaa
     6241 catccgccac cacgcggttg agcaattcgc cctgacgata gcgcgccagt ccggcagggg
     6301 ataggggcag caatttgctg aaggtgtaaa tgcgcagatg ctgcaacacg cggaaagtcg
     6361 cgtcgtgact taccagacgt tcaaaatagc gcccggcagt acgggtgatt gctgcgccac
     6421 gcacgcccgc agcgggtagc atatagttga agctgtacag tccggcaacc cccgctaccg
     6481 ctgaggcgga gaggaaccag ccggaaagtg tcaacagacc gatactggcg agcagcgtga
     6541 caattgccag cacaatacca agacttaaca tccatttatg acgtttatac agtgccagat
     6601 agggtagcaa agcgcgcatt taaatctcct cctgacgatg ggccagcaat gtggcgaaag
     6661 gaccaccagc cacgcttaat tccgcgtaac gtccttgctc aataatctga ccatcctgca
     6721 ttacccaaat gacatcccag tcagcaagat cttctaactg gtgggtgacc attaacgttg
     6781 tctggcgcag agaggcggca ttaagcgcct ccattacgcg ctgttcactg tgagcatcaa
     6841 ggctggcagc gggttcatcc aacagtaata gcgaacaggg atttagtaat gctcgggcca
     6901 ccgccacgcg ctgcgcctgc cccacggaaa ggcgggcagc ctggtcgcca acaggcgtat
     6961 caacgccttg tggcaggagc ggtagaaact cgctgaccca ggcgttatcc agcgctgttt
     7021 gtaactcgtg ttcgctggca tcaggtcgtg ccagtagtac gttatcccgt agtgttgctg
     7081 ccggtaattg tgggttttgc ccaacccagg agagatgctt acgccatgat tccggtgata
     7141 aatcgcgtaa ttctatcccg ttgattcgta gcgatccctg atatgagaga aaaccagaaa
     7201 gcgcgttcag cagtgagctt ttacctgaac cgctgcgacc aaccaacact gcacgttggc
     7261 ctgctggcaa agtaaagttc agcggtccag ccagcgtttt accttctggt gatgtaataa
     7321 acagatcctc ggcttcaatg gtcaccggat cggttgatgc taattccgcc tcaccacgtt
     7381 gcggatgggc tagcggggtt tccataaacg ttttcagact gtcagctgcg ccaacagcct
     7441 gggctttagc atgataaaac gtaccgagat cgcgtaatgg ctggaaaaac tccggcgcaa
     7501 ggatcagggc cagaaaaccc gcagccagcg tcacgccagt atcatagtga ccaaaatcca
     7561 gctcgccgag ataggaaaaa ccaaagtaga ccgccaccag agcaattgac agcgaggtaa
     7621 aaaattcgag aatgccggag gataaaaacg ccagccgtag cacttccatt gtccgttggc
     7681 ggaaatcttc cgaagcagaa cgaatacttt caatttcagc ttcaccacga ccaaaaatac
     7741 gcaatgtttc catgccgcgc aggcgatcga ggaaatgccc acttaagcga gcaagagcga
     7801 gaaagttacg tcggttagca tcggcagccc ccattccaac cagcgccata aacaacggaa
     7861 ttaacggtgc agtgcccagc agaataagcg ccgcagccca gttagacggg aagatcgcca
     7921 ccacaatcag caaaggcacg gacactgcca gcgccatttg cggcaggtag cgtgcatagt
     7981 aatcatgcat atcgtcaatt tgctcgagta ccagcgtcgc ccagctcccc gccggtttac
     8041 cctgaatcca cgctggccct gcttgttgca gacggtcgag aacctgacgg cggatggcaa
     8101 agcggatatg ctgcccggcg tgataaccca cccgttcgcg taaccagacc acccatgcgc
     8161 gcagtacaaa ggtcagaacc agtaacgtaa agggaagcag cagggcttca cggggaatat
     8221 tctccataat catatgttgc agaattcgcg ccatgaacca ggcctgggca atgatcaata
     8281 tgccgctcac aaagcccagc agacgagaaa tattcagcca acgttgggag atgacgcttt
     8341 gctgttttaa ccagcgggtt aactcttttt gacgggattt attcattgca cgcttagcag
     8401 gtgagttatc agaattattt gcagagcaat gttacaacga ggaaaaaata aaggcgaccc
     8461 atagtcgcat ggtgtcgcct tctttacttt tgttactgat ttgtaaaatt attttgcgtc
     8521 agctaaacca tcgaggtagc gttccgcatc aagtgctgcc atgcagcctg taccagcaga
     8581 tgtaatagcc tggcgataaa tgtgatccat cacgtcgcct gcggcaaaga cgccagggat
     8641 gctggtctgg gtggcattac catgaatacc cgactgtact ttgatgtagc cgttttccag
     8701 ttccagctgc ccttcgaaaa tagccgtatt cgggctgtga ccgatagcaa caaacagacc
     8761 ggcaacgtcg agtgactcga tgttatcgct gttttgcgta tcgcgcagac gaacgccagt
     8821 gacgcccatc tgatcgccgg tcacctcttc cagcgtacgg ttggtgtgta gaatgatgtt
     8881 gccgttctcc actttatcca tcagacgctt aataaggatt ttttccgcgc ggaaaccgtc
     8941 acggcggtga atcagatgca cttccgaagc gatgttagac agatacagcg cctcttcaac
     9001 cgcggtattg ccgccgccga tgaccgcaac tttctggttg cgatagaaga aaccgtcgca
     9061 ggtagcacaa gcagaaaccc cacggccttt aaaggcttct tcagatggca ggcctagata
     9121 gcgtgcagaa gctccggtgg caataatcag cgcgtcgcaa gtgtattcgc cgctatcgcc
     9181 agtcagacgg aacggacgat tctgcaaatc aactttgttg atgtgatcaa agatgatttc
     9241 agtttcaaat ttggtggcat gttcgtgcat gcgctccatt aataacggac cggtcagatc
     9301 gtttggatcg ccaggccagt tttccacttc cgtggtggtg gtcagttggc cgcctttttc
     9361 catgccggta atcagcacag gttgcaggtt ggcgcgcgcc gcgtagacag cagcggtata
     9421 tcccgccggg cctgaaccca ggataagcag tttactgtgt ttggtcgtgc ccatgagatc
     9481 cccatagttg ttggcagaca atgggcagga ttgtagggaa tttacagacg taaaaaaaga
     9541 gtatgacgat tttgttaaca atttgtgcaa tcggcagcat cgataagcag gtcaaattct
     9601 cccgtcatta tcacctctgc tacttaaatt tcccgcttta taagccgatt aaatgatgaa
     9661 taaacgcccc tgttaatgaa tatctggcat gttgtactaa aaatcgatgt tttgctttga
     9721 caatcccctg gtgttttgcg aaaacattcg aggaagaaaa aaacagtatt cttatatgcg
     9781 cataaccatg catgtaaata ccatgtttac cgtgctagtg aaatctacgt atggcgtgga
     9841 cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca ccgtagaccc
     9901 aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta gcgactctga
     9961 acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac aataataatg
    10021 gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat
    10081 gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt gggactttcc
    10141 ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat tcagggctat
    10201 acggcgctgc tgaaccccca ttatctggat gcatcacttc tggtattcgt tgagattact
    10261 ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa
    10321 gaaattcagg agtgtcattt agtatccggt gatttcgact acctgttgaa aacacgcgtg
    10381 ccggatatgt cagcttaccg taagttactg ggggaaaccc tgctgcgtct gcctggcgtc
    10441 aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg tctggttatt
    10501 aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat
    10561 ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca tcgggcagga
    10621 aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac aaagaagtca
    10681 cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc cttattgtcc
    10741 tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac cccagctggt
    10801 cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt gcgtggctgg
    10861 cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc attattgtcg
    10921 gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat tattttgccg
    10981 tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt ctggcggcaa
    11041 tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc ttactaagca
    11101 ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc tgcgtttggg
    11161 ctgcgggtct gacgctgttc accggttggt catgggtgac cattgctgaa aaactcggcg
    11221 gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat gatacctggg
    11281 tcgatgaaga tgagtatgaa gacgacgaag aatatgaaga tgaaaatcac ggtaaacagc
    11341 atgaatcacg ccgtgcccgt attcttcgcg gcgcgttagc gcgtcgtaaa cggttggcgg
    11401 aaaaattcat taatccgatg gggcggcaaa cagatgctgc gttgttctcc ggtaagcgga
    11461 tggatgatga agaagagatt acctacactg cacgcggtgt ggctgccgac cctgacgacg
    11521 tcctcttttc gggcaatcgt gcaacgcagc cagaatatga cgaatacgat ccattattaa
    11581 acggtgcgcc aattaccgaa cctgttgctg tagcagctgc tgctaccacg gcgacacaaa
    11641 gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct gttgatgttc
    11701 caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg ggagagccgg
    11761 tgattgctcc ggcaccggaa ggttacccac agcagccaca atatgcgcag cctgcagtgc
    11821 aatataatga gccgctgcaa caacctgtac agccacagca gccgtattat gcgccagcag
    11881 cggaacaacc tgtgcaacaa ccttactacg ctcctgcggc ggaacaacct gttcagcagc
    11941 cgtattatgc aactgcggcg gaacagtctg cgcaacagcc gtattacgcc cctgcgccag
    12001 aacaaacggc ggcaggtaac gcctggcaag ccgaagagca gcaatccact tttgctccac
    12061 agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag ccgttgtacc
    12121 aacagccgca acccgttgaa cagcaccctg ttgtggagcc tgagcccgtt gtagaagaga
    12181 caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag cgtgcccgtg
    12241 aacgtgaaca acttgcggcc tggtatcaac cgattcctga accggttaaa gaaccagaac
    12301 cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagtg gaagccgctg
    12361 ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg ggggctgccg
    12421 caaccgttgc cgcgccagtc ttcagtctgg caaatagcgc tggaccgcgt ccgcaggtca
    12481 aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg
    12541 aactggcgtc ttacggtatt aagctgccct cacagcgtgc ggcagaagaa aaagcccgtg
    12601 aagctcagcg caatcagtac gattccggcg atcagtataa cgatgatgaa atcgatgcga
    12661 tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc tatggcgaac
    12721 agtatcaaca tgatgtgccc gtgaacgcag aagatgcaga tgctgcggca gaggctgaac
    12781 tggctcgtca gtttgctcaa actcaacaac aacgttattc cggcgaacaa cctgctgggg
    12841 cgaatccgtt cactctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg
    12901 gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg caacaaccga
    12961 ttgcaccgca gcaacagtat cagcagccgc agcaaccagt tgcgccgcag ccgcagtatc
    13021 agcagccgca acagccggtt gcaccgcagc agcaatatca gcagccacaa caaccggttg
    13081 caccgcagcc gcaatatcag cagccgcaac agccggttgc accacagccg caatatcagc
    13141 agccgcaaca gccagttgcg ccgcagccgc atgataccct gcttcatccg ctgttgatgc
    13201 gcaatggtga cagccgtccg ttgcataaac cgacgacgcc gctgccttct ctggatttgc
    13261 tgacgccgcc gccgagcgaa gtggagccgg tagatacctt tgcgcttgaa caaatggcgc
    13321 gcctggtgga agcgcgtctg gctgatttcc gtattaaagc cgatgtcgtc aattattctc
    13381 cggggccggt tatcactcgc tttgaattga acctggcacc gggagtaaaa gcggcgcgta
    13441 tttctaactt gtcacgtgac cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg
    13501 aagttattcc tggcaaaccc tatgtagggc tggagttacc gaataaaaaa cgacaaaccg
    13561 tttatctgcg cgaagttttg gataacgcca aattccgcga taatccgtcg ccattaaccg
    13621 tggtgctggg taaagatatc gccggtgagc cggtggttgc cgatctggcg aaaatgccgc
    13681 acttgttggt tgcggggact accggttccg gtaagtctgt cggtgtgaac gcgatgatcc
    13741 tgagcatgct ttataaagca cagccagaag atgtgcgttt catcatgatc gacccgaaaa
    13801 tgctggagct ttcggtttat gaaggcatcc cgcatctgtt aacggaagtc gttactgata
    13861 tgaaagatgc cgccaacgcg ctgcgctggt gtgttaacga gatggagcgt cgttataaac
    13921 tgatgtctgc gctgggtgtg cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg
    13981 atcgcatgat gcgtccgatt ccagacccgt actggaagcc gggtgatagt atggatgcgc
    14041 atcatccggt gctgaaaaaa gaaccgtaca tagtggtgtt ggtggacgaa tttgccgacc
    14101 tgatgatgac ggtaggtaaa aaagtggaag agctgatagc acgtctggcg caaaaagccc
    14161 gtgccgcggg tatccacctc gtactggcaa cgcagcgtcc atcggttgat gttattactg
    14221 gtctgattaa agcgaatatt ccgacccgta tcgcctttac cgtatccagt aagattgact
    14281 cacgtaccat tcttgatcag gctggcgcgg aatcactgct gggtatgggg gatatgctct
    14341 actctgggcc gaactccacg ttgccggtac gtgtccatgg tgcttttgta cgcgatcagg
    14401 aagttcatgc cgtggtgcag gactggaaag cgcgtggtcg cccacagtat gttgacggca
    14461 tcacctccga cagcgaaagc gaaggtggtg cgggtggttt cgatggcgct gaagaactgg
    14521 atccgttgtt cgatcaggcg gtgcagtttg tcactgaaaa acgcaaagcg tcaatttcag
    14581 gcgtacagcg tcagttccgc attggttata accgtgcagc gcgtattatc gaacagatgg
    14641 aagcgcaagg gattgtcagc gaacaggggc acaacggtaa tcgtgaagtg ctggccccac
    14701 cgccgtttga ctaattaatg catcgtaggc cggataaggc gcggtagcgt cgcatccggc
    14761 actctaacaa ctgaaaattc agtattttct tctttcctca agctgattat tagcctggaa
    14821 tagagagtag agggaactcc cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa
    14881 aattgccatc acctgtgcat tactctcaag cttagtagca agcagcgttt gggctgatgc
    14941 cgcaagcgat cttaaaagcc gcctggataa agtcagcaac ttccacgcca gcttcacaca
    15001 aaaagtgact gacggtagcg gcgcggcggt gcaggaaggc cagggcgatc tgtgggtgaa
    15061 acgtccaaat ttattcaact ggcatatgac tcaacctgat gaaagcattc tggtttctga
    15121 cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa gctacggcaa cctggctgaa
    15181 agatgccacc ggtaatacgc cgtttatgct gattgcccgc aaccagtcca gcgactggca
    15241 gcagtacaat atcaaacaga atggcgatga ctttgtcctg acgccgaaag ccagcaatgg
    15301 caatctgaag cagttcacca ttaacgtggg acgtgatggc acaatccatc agtttagcgc
    15361 ggtggagcag gacgatcagc gcagcagtta tcagctgaaa tcccagcaaa atggggctgt
    15421 ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc acggtagatg atcaacgtaa
    15481 gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc ggataatact tttcaacctc
    15541 tggccgcgcg tatgcggcca gaaaatttag cacagtatat cggccagcaa catttgctgg
    15601 ctgcggggaa gccgttgccg cgcgctatcg aagccgggca tttgcattct atgatcctct
    15661 gggggccacc gggtaccggc aaaacaaccc tcgctgaagt gattgcccgc tatgcgaacg
    15721 ctgatgtgga acgtatttct gctgtcacct ctggcgtgaa agagattcgc gaggcgattg
    15781 agcgcgctcg gcaaaaccgc aatgcaggtc gccgcactat tctttttgtt gacgaagttc
    15841 accgtttcaa caaaagccag caggatgcat ttctgccaca tattgaagac ggcaccatca
    15901 cttttattgg cgcaaccact gaaaacccgt cgtttgaact taactcggca ctgctttccc
    15961 gtgcccgtgt ctatctgctg aaatccctga gtacagagga tattgagcag gtgcttactc
    16021 aggcgatgga agacaaaacc cgtggctatg gtggtcagga tattgttctg ccagatgaaa
    16081 cacgacgtgc cattgctgaa ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg
    16141 aaatgatggc ggatatggcc gaagtcgatg atagcggtaa gcgggtcctg aagcctgaat
    16201 tactgaccga aatcgccggt gaacgtagcg cccgctttga taacaaagga gatcgctttt
    16261 acgatctgat ttccgcactg cataagtcgg tacgtggtag cgcacccgat gcggcgctgt
    16321 actggtatgc gcgaattatt accgctggtg gcgatccgtt atatgtcgcg cgtcgctgtc
    16381 tggcgattgc gtctgaagac gtcggtaatg ccgatccacg ggcgatgcag gtggcaattg
    16441 cggcctggga ttgctttact cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg
    16501 cgattgttta cctgtcttgc gcgccaaaaa gcaacgctgt ctacactgcg tttaaagccg
    16561 cgctggcgga tgcacgcgaa cgtccggatt atgacgtgcc ggttcatttg cgtaatgcgc
    16621 cgacgaaatt aatgaaggaa atgggctacg ggcaggaata tcgttacgct catgatgaag
    16681 caaacgctta tgctgccggt gaggtttatt tcccgccgga aatagcacaa acacgctatt
    16741 atttcccgac aaacaggggc cttgaaggca agattggcga aaagctcgcc tggctggctg
    16801 aacaggatca aaatagcccc ataaaacgct accgttaatg ttatcgttgc ggtaatgttg
    16861 ttactgtatc cctgtggtcg caggctgtgg ccacatctcc catttaattc gataagcaca
    16921 ggataagcat gctcgatccc aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac
    16981 tggcacgccg gggctttaag ctggatgtag ataagctggg cgctcttgaa gagcgtcgta
    17041 aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg taactcccga tcgaaatcca
    17101 ttggccaggc gaaagcgcgc ggggaagata tcgagccttt acgtctggaa gtgaacaaac
    17161 tgggcgaaga gctggatgca gcaaaagccg agctggatgc tttacaggct gaaattcgcg
    17221 atatcgcgct aaccatccct aacctgcctg cagatgaagt gccggtaggt aaagacgaaa
    17281 atgacaacgt tgaagtcagc cgctggggca ccccgcgtga gtttgacttt gaagttcgtg
    17341 accatgtgac gctgggtgaa atgcactctg gcctcgactt tgcagccgca gttaagttga
    17401 ccggttcccg ctttgtggta atgaaagggc agattgctcg catgcaccgc gcactgtcgc
    17461 agtttatgct ggatctgcat accgaacagc atggctacag tgagaactat gttccgtacc
    17521 tggttaacca ggacacgctg tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt
    17581 tccatactcg tccgctggaa gaagaagcag acaccagtaa ctatgcgctg atcccaacgg
    17641 cagaagttcc gctgaccaac ctggtgcgcg gtgaaatcat cgatgaagat gatctgccaa
    17701 ttaagatgac cgcccacacc ccatgcttcc gttctgaagc cggctcatat ggtcgtgaca
    17761 cccgtggtct gatccgtatg caccagttcg acaaagttga aatggtgcag atcgtgcgcc
    17821 cggaagactc aatggcggcg ctggaagaga tgactggtca tgcagaaaaa gtcctgcagt
    17881 tgctgggcct gccgtaccgt aaaatcatcc tttgcactgg cgacatgggc tttggcgctt
    17941 gcaaaactta cgacctggaa gtatggatcc cggcacagaa cacctaccgt gagatctctt
    18001 cctgctccaa cgtttgggat ttccaggcac gtcgtatgca ggcacgttgc cgcagcaagt
    18061 cggacaagaa aacccgtctg gttcataccc tgaacggttc tggtctggct gttggtcgta
    18121 cgctggttgc agtaatggaa aactatcagc aggctgatgg tcgtattgaa gtaccagaag
    18181 ttctgcgtcc gtatatgaac ggactggaat atattggcta atacccaatt tttctgaatc
    18241 taaaaagcgc ctgcgggcgc tttttttgtc tccctttgat accgaacaat aattactcct
    18301 cacttacacg taatactact ttcgagtgaa aatctaccta tctctttgat tttcaaatta
    18361 ttcgatgtat acaagcctat atagcgaact gctatagaaa taattacaca atacggtttg
    18421 ttactggaat caatcgtgag caagcttgag tgagccatta tgaaaacgaa aatccctgat
    18481 gcggtattgg ctgctgaggt gagtcgccgt ggtttggtaa aaacgacagc gatcggcggc
    18541 ctggcaatgg caagcagcgc gttaacatta ccttttagtc ggattgcgca cgctgttgat
    18601 agtgcccttc cggcaaaatc agacgaaaag gttatctgga gcgcctgtac agttaactgt
    18661 ggtagtcgct gcccgctacg tatgcacgtt gtggacggtg aaattaaata tgttgaaacg
    18721 gataataccg gcgatgacaa ctatgacggc ctgcaccagg tgcgcgcctg tctgcgtggt
    18781 cgttccatgc gtcgccgtgt ctacaatccg gaccgcctga aatatccgat gaaacgagtc
    18841 ggggcgcgcg gtgaaggcaa attcgagcgc attagctggg aagaagccta cgacatcatc
    18901 gcgaccaata tgcagcgcct gatcaaagag tacggcaacg agtctatcta tctgaactat
    18961 ggtaccggaa cgctgggcgg caccatgacc cgctcctggc cgccgggaaa taccctggtc
    19021 gctcgcctga tgaactgctg cggcggttat ctgaaccatt acggtgacta ctcttccgcg
    19081 caaattgcgg aaggtttgaa ctatacctac ggcggctggg cagatggtaa cagcccgtcg
    19141 gatatcgaaa acagtaaact ggtagtgctg tttggtaata accctggcga aacgcgaatg
    19201 agtggcggtg gggtgactta ctatcttgaa caggcgcgcc agaaatctaa tgctcgcatg
    19261 atcatcatcg atccgcgcta taccgacacc ggtgccgggc gcgaagatga gtggatccct
    19321 attcgtccgg gaacagatgc cgcactggtt aacggtctgg cgtatgtcat gatcaccgaa
    19381 aacctggtgg atcaggcatt cctcgataaa tattgcgttg gctacgacga gaaaacactg
    19441 ccagccagtg cgccgaaaaa tggtcactat aaagcttata ttctgggtga agggccagat
    19501 ggcgtggcta aaacgccgga atgggcctcg caaatcactg gtgttccggc agacaaaatc
    19561 atcaaactgg ctcgtgaaat cggtagtacc aaaccggcgt ttatcagcca gggatggggc
    19621 ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta tctcgatgct ggcgattctg
    19681 accggtaacg ttggtattaa cggaggcaac agcggcgcgc gtgaaggttc atacagctta
    19741 ccgtttgtcc gtatgccgac cctcgaaaac ccgatccaga ccagcatttc gatgtttatg
    19801 tggaccgatg ccattgaacg tggcccggaa atgacagcgc tgcgtgatgg tgtgcgcggg
    19861 aaagataagc tggatgtgcc gatcaaaatg atctggaact atgccggtaa ctgcctgatt
    19921 aatcagcatt ctgaaatcaa ccgtacccat gaaatccttc aggatgataa gaagtgcgag
    19981 ctgattgtgg ttatcgactg ccacatgacc tcatcggcga aatatgctga catcctgctg
    20041 cctgactgca ccgcttcaga acagatggac tttgcactgg atgcatcctg cgggaatatg
    20101 tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct ttgaatgtaa gaccatctat
    20161 gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc aacagtttac tgagggccgt
    20221 acccaggaag agtggatgcg gcatctgtat gcccagtcgc gggaagcgat tcctgaactg
    20281 ccaacgtttg aagagttccg taagcagggg atctttaaaa agcgcgaccc acaagggcat
    20341 cacgttgctt ataaagcttt ccgtgaagat ccgcaggcaa atccactgac cacgccatcg
    20401 gggaaaattg agatttattc gcaggcgctg gctgacattg ccgctacctg ggaattgcca
    20461 gaaggtgatg tgatcgatcc actgccgatc tacacgccgg gctttgaaag ttatcaggat
    20521 ccgctgaaca aacagtatcc gctgcagctt acgggtttcc actataaatc tcgcgttcac
    20581 tcaacttacg gcaacgttga tgtgctgaaa gcagcttgcc gtcaggaaat gtggatcaac
    20641 ccgcttgatg cgcaaaaacg cggtatcaac aacggcgata aagtcaggat ctttaacgat
    20701 cgtggtgagg ttcatattga ggcgaaagtg acgccacgaa tgatgccggg tgtggtcgca
    20761 ctgggtgaag gtgcctggta tgacccggat acaaaacgtg tcgataaggg cggttgtatc
    20821 aacgtactga ccactcaacg tccgtctcct ctcgctaagg ggaatccgtc acatacaaac
    20881 ctggttcagg ttgaaaaggt gtaaggagta accgatgaca acccagtatg gattttttat
    20941 tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg gcttgtaaag actacaaaga
    21001 tttgacgcca gaagtcagtt tccgccgcat ttatgaatat gctggcggcg actggcagga
    21061 agataacggt gtctggcacc agaacgtgtt tgcctactat ctgtcgattt catgtaacca
    21121 ctgcgaagat ccggcttgta ctaaagtctg cccgagcggt gcgatgcata aacgtgaaga
    21181 tggttttgtt gtggtcgatg aagatgtgtg cattggctgc cgttactgcc atatggcttg
    21241 cccgtacggc gcaccgcaat ataacgaaac gaaaggccat atgaccaaat gcgatggttg
    21301 ttatgaccgt gttgcggagg ggaaaaagcc gatctgtgtt gaatcttgcc cgctgcgggc
    21361 gctggatttc ggccctatcg acgagctgcg taaaaaacat ggcgatctgg cggccgttgc
    21421 gccgttgccg cgagctcact ttaccaaacc gaatattgtg atcaaaccca atgccaacag
    21481 ccgcccgacc ggggatacca ctggctatct ggcaaacccg aaggaggtgt gagatgggaa
    21541 gtggatggca tgaatggccg ctgatgatct tcacggtctt cgggcaatgt gtagcgggtg
    21601 gttttatcgt tctggctttg gcgctgctca aaggcgacct gcgagcagaa gcccagcagc
    21661 gtgttattgc ctgcatgttt ggtttatggg tgctgatggg cattggcttt atcgcctcta
    21721 tgcttcatct tggttcaccg atgcgcgcct ttaactcgct caaccgggta ggggcttcag
    21781 cactcagtaa cgaaatcgcc agcggttcga tcttttttgc tgttggtggc atcggctggt
    21841 tgctggcaat gctgaaaaag ctgtcaccgg cattgcgtac gctgtggctg atagtgacga
    21901 tggttcttgg cgtcatcttt gtctggatga tggtgcgtgt gtataacagc attgataccg
    21961 ttccgacctg gtacagcatc tggacgccga tgggcttctt cctgacgatg ttcatgggcg
    22021 gcccgttact gggttacctg ttgctgagtc tggctggcgt cgatggctgg gcgatgcgtc
    22081 tgctgccagc catttctgta ctggcactgg tagtgagtgg cgtgatgtca gtgatgcagg
    22141 gcgcagagct ggcgactatt catagctctg tgcagcaggc cgcagcgctg gtgccggact
    22201 atggcgcgct gatgtcctgg cggatcgtgc ttttggctgt tgccctgtgc ttgtggattg
    22261 caccacagct aaaaggttat cagcctgcgg ttccgctact ttctgtatca ttcattctgc
    22321 ttctggcagg ggaattaatc ggtcgtggcg tattctatgg tttgcatatg accgtgggga
    22381 tggctgtcgc aagttaatca gtaacaaccg gggtttcggc cccggttcgc ttttatttct
    22441 gcttcgttaa cgtgtcataa ctggtcatca agttacgata atcaggaatg tggttagaga
    22501 acaacgtcgc cagtccttca atatcattac gccagtcgcg atgcagctcg catgccacgc
    22561 cgaaccatgt catcaattgc gcaccagctt gcgacatgcg atcccatgcc gaatgccggg
    22621 taatttcatt aaaggtccct gacgcgtcag tcacgacaaa gacgtcaaac ccttcttcaa
    22681 tggctgaaag cgccgggaat gccacgcaaa cttcggttac cacaccggca ataattaact
    22741 gttttttacc tgtcgctttg acagctttta caaaatcttc gttatcccag gcgttaatat
    22801 ttcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc tttcagttct ggaactaatg
    22861 ggccgttagg accggtttca aaactggtgg taagaatagt tggtaagttg aaatacttag
    22921 ccaggtcacc cagcgccagc acgttatttt taaacttatc gggttcgata tcccgtacaa
    22981 gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc agcatcattt ttatcaagac
    23041 gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt ctgaaatcgg
    23101 tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc tgtgaccatg
    23161 aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag atcaataaaa acaatcagat
    23221 gagattaatc gtctctccag cattagcgtt atgaatgagt aatataaatc gataatacct
    23281 ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt tttggcggtg gcatgatgcg
    23341 catgaaattt gaacttcctc acggttttaa ttcatgtcca cgtatacccg gcctgtcatg
    23401 cttttgctgt ctggcctgct tttgttgact ctggcgatcg cagtgttaaa tacactcgtg
    23461 ccgctttggc tcgcccagga acacatgtcc acatggcagg taggcgttgt cagctcatcc
    23521 tattttaccg gcaacctggt cggtacattg ctgacagggt atgtcattaa gcgcattggc
    23581 tttaaccgca gctattatct ggcctccttc atttttgccg ctggctgtgc cggccttggc
    23641 ctgatgattg gattctggag ctggttggct tggcgttttg tcgccggtgt cggttgtgcc
    23701 atgatttggg tggttgttga gagcgcgctg atgtgcagtg ggacgtcacg taaccgtggg
    23761 cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa cgtttttagg ccagttactg
    23821 gtcagcaaag tttcaaccga gctgatgtcc gtattgccgt gggttacagg tttgacgttg
    23881 gcagggatct taccgctgtt gtttacgcgt gtgctgaatc agcaggctga aaaccatgat
    23941 tcgacgtcaa ttacgtcaat gctaaaactc cgtcaggcgc ggcttggcgt gaatggctgc
    24001 attatctcag gaatcgtgct gggatctcta tatggcctga tgccgctgta cctcaatcac
    24061 aaaggggtga gcaatgccag cattggtttc tggatggcgg tactggtcag tgcgggtatc
    24121 ctcggacaat ggccgatcgg acgtctggcg gataagtttg gtcgactgct ggtgttgcgt
    24181 gttcaggtct ttgtcgtcat tctcggcagt atcgcgatgc ttagccaggc ggcgatggcc
    24241 ccagcgttat tcatcctcgg tgccgctggc tttacgctat atccggtggc gatggcctgg
    24301 gcttgtgaga aagttgaaca tcatcaactg gtggcgatga accaggcctt actgttgagc
    24361 tataccgtgg gaagtctgct tggcccgtca tttaccgcta tgctaatgca gaatttctcc
    24421 gataatttat tgtttatcat gatcgccagc gtatcgttta tctatttgct gatgctgctt
    24481 cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt aaatgaattc aagcagagtg
    24541 tgaacttact gtttcacact ctgctttttt gtttcttcta tctgacttgc tttattccaa
    24601 attttattcg tttaaaaata aaatgtgcag caggttataa ttttgcattt cgctatttcc
    24661 gcacttctta tttgccgcgc ataatccctc gttttacaga tgccccttta attttggcga
    24721 aggatttgtc tatggctggg aatgttcagg aaaaacagct gcgatggtac aacattgcgc
    24781 tgatgtcttt tatcactgtc tggggttttg gcaatgttgt taataactac gccaaccagg
    24841 ggctggtggt tgttttttca tgggtgttta tctttgcgct ctatttcaca ccttatgcgc
    24901 taattgttgg tcagttaggc tcgaccttca aagatgggaa gggcggggtc agtacctgga
    24961 ttaaacacac gatgggaccc ggactggctt acctcgccgc gtggacctac tgggtagtgc
    25021 atattcccta tctggcacaa aaaccccagg caatactgat tgcgctcggt tgggcgatga
    25081 aaggcgacgg ttcgttaatc aaagaatatt cagtcgtagc gttacagggg ttaacgctgg
    25141 tgctgtttat cttctttatg tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg
    25201 gttctgtggc agggattgcg atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa
    25261 ccgcgcctgc aattactgaa gtgcatattg cgaccacaaa cattacctgg gaaacgttca
    25321 ttcctcatat cgactttacc tacattacca ctatttcaat gctggttttc gcggttggcg
    25381 gagcagagaa gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa
    25441 aagggatgtt atgcctggcg gtgatggttg cggtttgtgc cattctgggt tcactggcga
    25501 tggggatgat gtttgactcg cgtaatatcc cggatgactt aatgactaac ggtcagtatt
    25561 acgcctttca gaagctgggc gagtattaca acatgggtaa tactttaatg gtgatttacg
    25621 ccattgcgaa taccctggga caagtagctg cgctggtatt ctcgattgat gccccgctta
    25681 aagtgctatt aggcgatgct gacagcaaat atattccagc cagtttatgt cgtaccaacg
    25741 cttctggtac gcccgttaat ggctattttc tgaccctggt gctggtggcg atcctgataa
    25801 tgctaccgac tctcggcatt ggtgatatga acaatctcta taaatggctg ttgaatctta
    25861 actcggtagt gatgccgctg cgttatttat gggtatttgt tgcatttatt gcagttgttc
    25921 gcctggcgca gaaatataag ccggaatatg tctttattcg taataagccg ctggcaatga
    25981 ccgtcggaat ttggtgtttt gcctttaccg cttttgcctg cctgactggt attttcccga
    26041 aaatggaagc cttcaccgca gagtggacat tccagttggc gctgaatgtt gcaacgccgt
    26101 ttgtgctggt aggattaggg ctgatattcc cgctgctggc gcgtaaagcg aatagcaaat
    26161 aattattgtg gtcggtgatg ctgactctgg cagatctgac ccgtttttac ataatcgaga
    26221 aatgcgcgca agccgcagga catatactgg cggtttgggt aatacatttg gaagctgtgc
    26281 tgtaagggcg ttagaaatag agctctgaat tatttatgca gcgcggaata aaaaagcggc
    26341 aactcaaaaa agttgccgct ttacggggaa attagaacat taccttatga ccgtactgct
    26401 caagaatgcc tttcacgcgt tccatggtct ctttcttcgg tggtttaaca ccatcaagtt
    26461 tgtattcttc acccatcgcc acccatttgt gtttgcccag ttcgtggtag gggagaagct
    26521 cgattttctc aacgttgccc atatcacggg taaattcacc gaggcgatgc gctgaatcgt
    26581 catcgtcaga ccagcctggg acaacaacgt agcggatcca caccttcaca tttttgttcg
    26641 ccagatattt ggcgaactcc agcgtgcggt ggttggaaac tccaaccaga ttttggtgga
    26701 tctcgtcgtt catctgtttg agatcgagca ttaccaggtc ggttacttcc agcagttcat
    26761 caatcaccgg atcgtaacga cgaacaaaac cgttggtgtc cagacaggta tgaatgcctt
    26821 cttttttgca ggcgcggaac cagtcacgaa caaactcagc ttgcaggatt gcttcaccgc
    26881 cggatgcggt aacgccgccg ccggaagcgt tcataaagtg gcgataggtc accacctctc
    26941 tcattaaatc ttcaacggta acttctttac cgccatgcgt gtcccaggtg tcgcggttat
    27001 gacaatacag gcagcgcatc aggcagccct ggaaaaaggt gataaagcga atacccggac
    27061 cgtctacggt tccacaggat tcaaaggagt gaatgcgacc aataactgac attgcggtgt
    27121 ttctccagat gtggcccatc tgaggccgtg ttggtgcgca gctcgaaggc tacgtcgagt
    27181 ctgttttggc agtcacctta aagtatagat agctgacaaa aaaggctctc gtgctaaaaa
    27241 aggccccact ttcgtggagc ctttattgta cgctttttac tgtacgattt cagtcaaatc
    27301 taattacata gattgagtga aggtacgagt aataacgtcc tgctgctgtt ctttagtcag
    27361 cgagttgaaa cgtactgcgt agccagatac acggatggtc agctgcggat atttttccgg
    27421 gttttccatc gcgtcgagca gcatttcacg gttcatcacg ttaacgttca ggtgctgacc
    27481 accttcgatg gacgcttcgt ggtggaagta accatccatc agaccagcca ggttggtctt
    27541 acgaacttcg tcgtctttac ccagtgcgtt cggaacgata gagaaggtgt aggagatacc
    27601 atctttagcg taagcaaacg gcagtttagc aacggaagtc agagaggcta ctgcaccttt
    27661 ctggtcacga ccgtgcatcg ggttagcacc cggtccgaac ggcgcgccag cacgacgacc
    27721 gtctggggtg ttaccagttt tcttaccata cacaacgtta gaagtgatgg tcagaacaga
    27781 ctgagtcggg atagcgtcac ggtaggtgtg cagtttctga attttcttca tgaaacgttc
    27841 taccaggtca acagccaggt catctacacg cggatcgttg ttaccaaact gcgggtattc
    27901 gccttcgatt tcgaagtcga tagccagacc gtcttcgtca cgaatcggtt taactttcgc
    27961 atatttgatt gcagacaggg agtcagcagc aacggacaga ccagcgatac cacacgccat
    28021 ggtgcggata acgtcacggt cgtgcagcgc catcagagag gcttcgtagc tgtacttgtc
    28081 gtgcatgtag tggatgatgt tcagtgcagt gatgtactgt ttagccagcc agtccatgaa
    28141 gtgatccatg cgctccatca cttcatcgta gttcaggaga tcgcctttga tcggctcaga
    28201 cttcggacca acctgcattt tcagtttttc gtcaacgccg ccgttgattg cgtacagcat
    28261 ggttttcgcc aggtttgcac gcgcaccgaa gaactgcatt tgtttaccaa cgatcatcgg
    28321 gcttacgcag caagcgatag cgtagtcatc gttgttgaag tccggacgca tcaggtcatc
    28381 gttctcatac tgcagagaag aggtgtcgat ggacacttta gcagcgaatt tcttgaagtt
    28441 cagcggcagt ttttcggacc acagaatggt catgttcggt tccggagacg gacccatggt
    28501 gtacagggtg ttcaggaaac ggaagctgtt tttggtaacc agggtacgac cgtcgaggcc
    28561 cataccaccg atagattcgg ttgcccagat cgggtcgcca gagaacagtt catcgtattc
    28621 cggagtacgc aggaagcgaa ccatacgcag tttcatgacc aggtggtcaa ccatttcctg
    28681 cgcttcttgt tcggtgatct tgccagcttt caggtcacgt tcgatgtaca catccaggaa
    28741 ggtggaggta cgaccgaagg acattgcagc accgttctga gacttaacag cagccaggta
    28801 gccgaagtaa gtccactgga tagcttcctg agcgttggta gccggaccag agatgtcgta
    28861 gccgtatttc gcagccattt ctttcatctg acccagagcg cggtgctgtt cagcgatttc
    28921 ttcgcgcaga cggatagtct gttccaggtt tacgccgttt tccagatcag cctgcagaga
    28981 ggtgaactga gcgtatttgt ctttcatcag atagtcgata ccgtacagcg caacgcgacg
    29041 gtagtcaccg atgatacgac cacggccata agcatctggc agaccggtca gaacaccgga
    29101 tttacggcaa cgcaggatgt ccggagtgta aacgtcgaac acgccctggt tgtgagtttt
    29161 acggtattca gtgaagattt ttttgatcat cgggtccagt tcgcggttgt acgctttgca
    29221 ggaaccttcg atcattttga taccaccgaa cgggataaga gcacgtttca gcggagcttc
    29281 agtctgcaga ccaacaactt tttccaacgc tttgttgatg tagccagcgt cgtgagaggt
    29341 gatggtggaa gcaacagcgg tgtcaaagtc aactggcgcg tgagtgcggt tttccagttt
    29401 aacgccttcc attactttgt cccacagggt ggtggtcgct tcagtagcgc cagccaggaa
    29461 ggactcgtca ccctcgtacg gagtgtagtt tttctgaatg aagtcacgga cgtttacttc
    29521 attctgccag tcacctttgg taaaaccttc ccaggctgtg gctaactttt cattaagctc
    29581 ggacatgtaa cacctacctt cttaagtgga ttttttattt actgcgtact tcgacaacca
    29641 ttaatggtga tcgttttcac gcagataaat gacccagtat gtcaacccaa ccaacaaacc
    29701 accgccgata atattaccga tcgtaaccgg aatcaggtta tcagtgatga agttcatcac
    29761 ggtcaggtga gaaaaatttt ccggtgcaga accgacagcg gtccagaatt ccggagatgc
    29821 gaagtcgcgg attacaatac ccatcgggat cataaacatg tttgcgatac tgtgctcaaa
    29881 accgctggca acaaacatcg cgaccggcag caccataatg aacgctttat ccatcaggct
    29941 gcggccagaa taactcatcc atactgccag acataccatc aggtttgcca ggataccaag
    30001 acagacggcc tcaataaaag tatggtgcac tttgtggtcg gcggtttgta ggacgtttag
    30061 tccccattga ccattcgcgg tcatatactc gccggaaagc cacattaaaa gtacaaacag
    30121 cagtgcgccg accaggttgc caaaatagac atttagccag tttttcgcta actgacccca
    30181 ggtgatgcgc ccactcgcct tagcaacaac aatcaacact gtggaagtaa agagatcggc
    30241 tccgcagaca acacaaagaa tcagccccag agagaagcaa atgccgccaa ccagttttgc
    30301 catgccgaag ggcattgttc ctgtgccagt ggttgctgtg atatagaaga cgaatgcgat
    30361 tgagatgaaa acaccggcgg taatcgccag atagaaagtc ttaagcggat gtttcgttgc
    30421 tttatagaca cctgcctctt cggccacttt ggccattgct gcaggaagta aaagatcaaa
    30481 agggttgtca gctttcacac taactctctc tttattaagt cggcgacgag atactaacaa
    30541 agcattatag atgagaaatt gatatagatc atatctcgcc tggcttatag gcccgtaact
    30601 cgcatggttt ttatgcaaat acggagtaaa tatttgatta tccaaataaa aataaatttt
    30661 aaaaattaac aaatgagttg aattttttcc gcatcctccg ctaaaacagt taatt
//
LOCUS       DAZGAP010000037        30156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGAP010000037 DAZGAP010000000
VERSION     DAZGAP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.037"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHLAM_001427"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHLAM_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO7852983.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHLAM_001428"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHLAM_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO7852984.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHLAM_001429"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHLAM_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO7852985.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHLAM_001430"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHLAM_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO7852986.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHLAM_001431"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHLAM_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO7852987.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHLAM_001432"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHLAM_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO7852988.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHLAM_001433"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHLAM_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO7852989.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHLAM_001434"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHLAM_001434"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO7852990.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHLAM_001435"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHLAM_001435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO7852991.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12916..14049)
                     /gene="thiH"
                     /locus_tag="ACHLAM_001436"
     CDS             complement(12916..14049)
                     /gene="thiH"
                     /locus_tag="ACHLAM_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO7852992.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQVKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14046..14816)
                     /gene="thiG"
                     /locus_tag="ACHLAM_001437"
     CDS             complement(14046..14816)
                     /gene="thiG"
                     /locus_tag="ACHLAM_001437"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO7852993.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVIPLGAPIGSNQG
                     LETRAMLEIITQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14818..15018)
                     /gene="thiS"
                     /locus_tag="ACHLAM_001438"
     CDS             complement(14818..15018)
                     /gene="thiS"
                     /locus_tag="ACHLAM_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO7852994.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQSGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15002..15757)
                     /gene="thiF"
                     /locus_tag="ACHLAM_001439"
     CDS             complement(15002..15757)
                     /gene="thiF"
                     /locus_tag="ACHLAM_001439"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO7852995.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRNLALRRASGCPVCGGSNADPV"
     gene            complement(15750..16385)
                     /gene="thiE"
                     /locus_tag="ACHLAM_001440"
     CDS             complement(15750..16385)
                     /gene="thiE"
                     /locus_tag="ACHLAM_001440"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO7852996.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16385..18280)
                     /gene="thiC"
                     /locus_tag="ACHLAM_001441"
     CDS             complement(16385..18280)
                     /gene="thiC"
                     /locus_tag="ACHLAM_001441"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO7852997.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGREHIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18361..18461)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18513..18989)
                     /gene="rsd"
                     /locus_tag="ACHLAM_001442"
     CDS             complement(18513..18989)
                     /gene="rsd"
                     /locus_tag="ACHLAM_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO7852998.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19084..19857
                     /gene="nudC"
                     /locus_tag="ACHLAM_001443"
     CDS             19084..19857
                     /gene="nudC"
                     /locus_tag="ACHLAM_001443"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO7852999.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19897..20961
                     /gene="hemE"
                     /locus_tag="ACHLAM_001444"
     CDS             19897..20961
                     /gene="hemE"
                     /locus_tag="ACHLAM_001444"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO7853000.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHL"
     gene            20971..21642
                     /gene="nfi"
                     /locus_tag="ACHLAM_001445"
     CDS             20971..21642
                     /gene="nfi"
                     /locus_tag="ACHLAM_001445"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO7853001.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21685..22275
                     /gene="yjaG"
                     /locus_tag="ACHLAM_001446"
     CDS             21685..22275
                     /gene="yjaG"
                     /locus_tag="ACHLAM_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO7853002.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22462..22734
                     /gene="hupA"
                     /locus_tag="ACHLAM_001447"
     CDS             22462..22734
                     /gene="hupA"
                     /locus_tag="ACHLAM_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO7853003.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22747..23442
                     /gene="yjaH"
                     /locus_tag="ACHLAM_001448"
     CDS             22747..23442
                     /gene="yjaH"
                     /locus_tag="ACHLAM_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO7853004.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23444..23863)
                     /gene="zraP"
                     /locus_tag="ACHLAM_001449"
     CDS             complement(23444..23863)
                     /gene="zraP"
                     /locus_tag="ACHLAM_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO7853005.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMDHW"
     gene            24101..25477
                     /gene="zraS"
                     /locus_tag="ACHLAM_001450"
     CDS             24101..25477
                     /gene="zraS"
                     /locus_tag="ACHLAM_001450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO7853006.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQVGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25474..26799
                     /gene="zraR"
                     /locus_tag="ACHLAM_001451"
     CDS             25474..26799
                     /gene="zraR"
                     /locus_tag="ACHLAM_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO7853007.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26796..28085)
                     /gene="purD"
                     /locus_tag="ACHLAM_001452"
     CDS             complement(26796..28085)
                     /gene="purD"
                     /locus_tag="ACHLAM_001452"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO7853008.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28097..29686)
                     /gene="purH"
                     /locus_tag="ACHLAM_001453"
     CDS             complement(28097..29686)
                     /gene="purH"
                     /locus_tag="ACHLAM_001453"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO7853009.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7055 a         7828 c         8318 g         6955 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggtgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa tacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtagcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 aaccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagagttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgttgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggcgttgttg aagaagttga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga cccctgtaga gctggacttc agccaggttg
     1381 aaaaagctta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgcgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcactg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgagag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgctctgct gaaagaactg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 gggtgcaaac gctgaagctg ctaaagctgc aggtgctgaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggtaactg agtgagtcgc caggagcata gttaactatg
     2821 tgactggtgc gaacgaagga agccaacgcc gccacaagct gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggcttg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgt
     5281 gctgcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tattctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaata gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctatctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cctgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgacaagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgatatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccaga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggttac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcgtct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcatggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttctgg tcagatccgc
     7981 ctgtacgacg gccgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt actaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggtgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggcctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt acttcgaatc
     8881 ctatgtggtt attgaaggcg gtatgaccaa cctggaacgc cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaaa tgggtgcgga
     9001 agcaatccag gccctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gcagttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcctc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggctttg catatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtt attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa cggcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtact catgaaggta tcatgatgac
    10921 tccggttatc gaaggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcaaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga ccgacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gatgtactga tcccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatctggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 tctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcactccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccaaaatg gcgtcagctc aatgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgatgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaagacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggtgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aactcgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaattctt cacggaattt ttaagaaagg cagggcgaaa
    12781 cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cgggactacc ataaaaaaac
    12841 acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg
    12901 ttgttacatt ctgtctcatg gcctttgcga ggcgcgtccc agatagctgt cccagtcttt
    12961 ccataccggc tgcaaaccct gagcagttaa cgcggcagca accgcttccg gtctgcgatc
    13021 gtcgtgcggt gagaactgtt ccagctcggg gtgattgtcg gcatagccac ctggctgcgt
    13081 tttcgaaaaa gcgctgacgt tattaattgc cagcggaata acgcgatcgc gaaaccacgg
    13141 tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagccgga aggcgcagat
    13201 ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacatgg
    13261 acgcaggcgt gggaaggaga tagagtagcg gctttgccag taatgctgtt gcagccatag
    13321 caaatgttct gcaaccatat agcagtcaac gcgccagctg tcggaaaggc caattagcgc
    13381 gccgaggcct atcttatcaa tccccgcacg gcccagccga tccggcgttt ccagccgcca
    13441 gaagaagtcc tgttttttac ctttcagatg atggcgggca taagtcgcct cgtgatatgt
    13501 ctcctgataa accatcacgc catccagacc tagttgcttt aactcggcgt attccgtctc
    13561 cgccagcggt tgcacttcca tctgtagtga agagaactgt tcacgcaggg cagggagatg
    13621 acgacgaaag taatccatcc ccactttcac ctgatgttca ccagtgacta atagcagatg
    13681 ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt
    13741 tttgcgcttg atgcgattgc tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag
    13801 attggaaaga taaagcggga cgtagaagct gacgacgttg ccaaatcgct gacgggtcag
    13861 acgctgcgcc cgttgggcca gtggttccag atagccactg gcggctggcg ataacagcgc
    13921 catcatatcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt
    13981 tttgctgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc gatcgctgaa
    14041 ggttttcatg ctgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaatga
    14101 ctgcggctgc ccggtccgga ctgacgtgcc agcaggcctg cttctactgc cagacgaaat
    14161 gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg
    14221 tcggccccca tttccagcgc ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca
    14281 accaccggaa ccgtggcttg ctgggtaata atctccagca tggcacgggt ttccagtccc
    14341 tgattcgagc caatcggcgc gccgagcggt atcaccgctg cacagccgac ttcttccaga
    14401 cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc
    14461 agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg
    14521 tgaatctcta attttaacca gtttgtgcct aacgcttcac gagccagatg ggcggcgaaa
    14581 atggcttctt ccgctgtttt cgccccggat gtatttggca gcagggtcac acccgccgcg
    14641 ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt tgacgcgttt catcgccagt
    14701 gtcaccagtt ggctgccgca agcgcggatc gcctccatca tcagttgtga agaggcgaat
    14761 ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca
    14821 accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgc
    14881 ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg attgtcgttg
    14941 gtcgagttgc tccagtagtt cgtgaaccgt ttgcccggcg gcgcactgca tcggttgatc
    15001 gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg
    15061 ccaggttgcg ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggcgtct
    15121 ctataccgct taataactta atggcttcca gtgcctgtaa agtgcccata accccgacca
    15181 ccgggccaac cacgcccgcc gtgcggcagt tgcgttcagg ctcctggtta tctggccaca
    15241 ggcagcggta acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc
    15301 cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca ttaatctcct
    15361 gacgagtcgc catattgtcg gtacagtcga gcaccacatc ggcccgtgct accgcatctt
    15421 ttaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatatcg ggattcaact
    15481 gtgtcagtcg ctgatggctg acctgcgatt tcgggcgatc gatatcttca gtggtaaaga
    15541 ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc
    15601 cgacgccagc gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa
    15661 tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagga
    15721 tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc aatttccagc
    15781 aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgcttac gacggcgatg
    15841 ctgccgacac ccgttgctat caccgcaggc gcgcgcgcca gactgatacc gccaatcgcc
    15901 acggtgggat aatccgccag gcgctcaaca tgccgtgcca gctgttccag cccctgcggt
    15961 gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg
    16021 cgtgctgcca gcgcgacgtc gatctccata tcgtcatggg tcgaaacgcc cagtcgcaga
    16081 cctgccgcgc ggatggcgct gagatcggtg gcttgcaaat cttcctgccc cagatggacg
    16141 ccatatgcct gatgcttgat cgccagccgc cagtaatcgt tgataaacaa tcgcgcgtta
    16201 tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgtcga
    16261 tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt
    16321 acgctgtcca ccaccgggta cagtcctaaa cgaaagggta caggaggaaa atctggctga
    16381 tacatcacgc ttcctcctta cgcaggtaga tttctccgcc tctggcgcgg aagttctccg
    16441 acatatcagc cattcccact tcaatggttt gcgcggcggc gtaatcgcgc acttcctggc
    16501 tgattttcat cgagcagaat ttcggcccac acatggagca aaaatgggcg actttgccgg
    16561 attcttgcgg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat
    16621 taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt
    16681 gcgcgcccgg atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa
    16741 gcccctgctt aacatcttct ttattaggca gacccagatg ctcttttggc gttacgtaac
    16801 agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt
    16861 cataacccgg cgcgatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc
    16921 agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc
    16981 cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttca cccagcgtat
    17041 gcagctcggc aaattgcgct tcatcgttgg cgtcctgaat agaaccaggg cgcagaccgt
    17101 ctcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt
    17161 gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc
    17221 gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc agtacgcccg
    17281 catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg
    17341 cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca
    17401 ctgtaccgat cggcaccggg ctgttacgca aaatccactc gcgggtttcg tgaatatagc
    17461 gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt
    17521 ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta
    17581 ccaggaaatt gcgaccaata atcatcggct ccgattccgg atgattaatg ttggccggga
    17641 taatcgcgcg tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca
    17701 gacgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgtgct
    17761 cgcggcccat attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc
    17821 gggcgtagtg cagttgggtg acgcggcgtc ctgctttggc gcgttttggt gttagtacgc
    17881 cgctaaaacg cagctcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac
    17941 tgcgcacggt aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg
    18001 ccagtccttg ctgcacgtta atggcgatct gtggatcgcc atacggaccg gaggtgtcgt
    18061 agaccggaat cgcttcgttt tcttcgtact gcggttgttc tttgctgccg ccaattagcg
    18121 tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg ccagtgatgt
    18181 aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggcct
    18241 gggcgcgttg ttcgcggcgg gtcagttttg ttacagacat agctcattcc aaaaagttaa
    18301 ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat
    18361 agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga
    18421 ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagccccaca aacggggcgt
    18481 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18541 tcaagtacca gcagaatcaa cttatcttcc agcacaaagc gtgcttccag cgcctcgccg
    18601 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    18661 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    18721 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    18781 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    18841 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    18901 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgt
    18961 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19021 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19081 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19141 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19201 ggtcagcgcg cactacaaat cggcgaatgg caaggggaac ctgtttggtt agtacaacag
    19261 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt agggctgttt
    19321 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19381 tactgcgggc atgaaatgta tccaagcaaa accgaatggg cgatgctgtg cagccattgc
    19441 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19501 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19561 ttcgtcgaag tgggtgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19621 ggaattaaag ttaaaaactt gcgttacgtg acctcccaac cgtggccgtt tcctcagtcg
    19681 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgttatcga cccgaaagaa
    19741 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    19801 gtagcgcgcc gtctgataga agatacggtg gcgatgtgtc gggcagagta tgagtgatga
    19861 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    19921 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    19981 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20041 ctgtgcaaaa atgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctatccg
    20101 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cagacgcgat gggtttaggg
    20161 ctctattttg aagccggaga aggtccgcgt tttacctcac cagtcacctg caaggccgac
    20221 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20281 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20341 tggacgctgg cgacctatat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    20401 aaaatgatgt atgccgatcc acaggcgctg cacgcgctac tcgataaact ggcgaaaagc
    20461 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggctgtgat gattttcgac
    20521 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20581 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtaccggt cacgctgttt
    20641 accaaaggcg gcggacagtg gctggaagcc atggcagaaa ccggttgcga tgcgctgggc
    20701 ctcgactgga caacggacat cgccgatgca cgccgccgcg tgggcaataa agtcgcgttg
    20761 cagggcaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    20821 gcgactatac ttgcaggttt cggtcacggt gaaggtcatg tctttaacct tggtcacggc
    20881 attcatcagg atgtgccgcc agaacatgct ggcgtgtttg tggaggcagt gcatcgcctg
    20941 tctgcgcctt atcatctgta aggagtggtt atggatctcg cgtcattacg cgctcaacaa
    21001 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21061 atcgccggag ccgatgtcgg atttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21121 ctgctgaaat acccctcgct tgagctggtg gagtataaag tggcccgcat cgccaccacc
    21181 atgccttaca ttcctggttt tctctccttt cgtgaatatc ctgcgctgct ggcagcgtgg
    21241 gagatgctgt cacaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    21301 cgccgtcttg gcgtcgccag ccattttggt ttgctggtgg atgtgccgac cattggcgta
    21361 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    21421 ccgctgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    21481 ccattgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    21541 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    21601 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    21661 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    21721 ctggaaagct ggcagcacgt cacttttatg gcctgcttat gcgaacgtat gtaccccaat
    21781 tacgccgtat tctgtcagca aaccggcttc ggcgatgggc agatttaccg ccgcattctc
    21841 gatctcattt gggaaacgct gaccgtcaaa gacgcaaaag taaatttcga cagccaactg
    21901 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    21961 gcaatcgatg cttgcgtggc gttaagtgaa ctggtccatt cgcgtttaag tggcgaaacg
    22021 ctcgaacacg cggtggaagt gagtaagact tccatcacga ccgtggcgat gctggaaatg
    22081 actcaggctg gtcgcgaaat gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa
    22141 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22201 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22261 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    22321 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    22381 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    22441 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    22501 gcagaactgt ccaaaaccca ggctaaagct gctctggagt ccactctggc tgcaattact
    22561 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    22621 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    22681 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    22741 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    22801 tcattgatgc tggctggcgc tctgtttctc actgcttgta gtcacaactc ttcacttcct
    22861 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    22921 agcggcgata atgtgcatct tcttgccgtg tttagcccgt ggcgcagtgg cgatacgacg
    22981 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23041 aaacccccgg tgaatattcg cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa
    23101 cgcgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23161 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23221 caggggcgct ggcatgcgat ggagcagact gtgaccacct gcgaagggca aaccattaaa
    23281 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    23341 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt ggtggcaaac
    23401 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggtccatac
    23461 ccatatgacc gccaccaccg cagccgccgt aacccattcc ggtaccgcgc ggaatacccg
    23521 cttcagccat cgcaatatct cgtttcaccc gtaactcatc taacgactga cgcaaattct
    23581 ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg gctaacaggg
    23641 cattgtattc ataacgcttt gttaccagtt gctgttgcag tgcgctgctt tgagcgtaaa
    23701 agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt
    23761 gctgccacat accgtgtccg ccgtgagcaa atgccgatgt cgatcccatc gccattgctg
    23821 aaagcgccat cattaccagg gcaattttcg tgttccgttt catggttaat cctccagtgg
    23881 ttgtcttact tcgggtattg catcttccgt gccaacgatg aaacgctgat atgacgggta
    23941 atctggcatg ataaacgagt aaaaatgact cgcctgctgc gggtagcgag tcatttttac
    24001 tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa agatggcatg
    24061 atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta tgcaacgttc
    24121 taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg ttgggctggt
    24181 ggggctgttt gcggtgacag tgattcgcga ttacgggcgc gagactgcag ccgccagaca
    24241 aacgctgctg gaaaaaggca gtgtacttat ccgtgctctc gaatccggct cgcgcgtcgg
    24301 catggggatg cgcatgcatc atgcgcagca gcaggcatta ctggaagaaa tggccgggca
    24361 gcctggtgta cgttggtttg cggtcacgga tgaacaagga acaatcgtga tgcatagcaa
    24421 ctccggcatg gtgggaaaac agctttattc cccgcaggaa atgcagcagt tacatccggg
    24481 agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc tggaaattta
    24541 tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg atgcgccata tgcagcagta
    24601 tgccgcgaca ccacaagcaa tttttatcgc tttcgacgcc agtaacattg tgagtgccga
    24661 agatcgtgag cagagaaaca ccctgattat cctcttcgcc ctggcgacgg tcttgctggc
    24721 aagcgtgttg tcattcttct ggtatcgccg atatctgcgc tcgggccagc tgttgcagga
    24781 tgaaatgaag cgcaaagaga agctggtggc actggggcat cttgcggcag gcgttgccca
    24841 cgaaatccgt aacccacttt cctcgattaa agggctggcg aaatactttg ccgaacgcgc
    24901 gccagcaggg ggagaagcgc atcaactggc gcaggtgatg gcgaaagaag ccgaccgttt
    24961 aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca acgcatctgg ctttgcaggc
    25021 ggtggatctc aacacgctga ttaaccactc attacagctg gtaagccagg atgcaaacag
    25081 ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc aggccgatcc
    25141 ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg cgattggtca
    25201 gcatggcgtg attagcgtga cggccagcga aagcggcacg ggtgtgaaaa tcagcgttac
    25261 cgacagcggt aagggaattg cggcggatca gcttgaagcc atcttcaccc cgtatttcac
    25321 cacgaaggcc gaaggcaccg gactggggct ggcggtcgtg cataatattg ttgaacaaca
    25381 cggtggtaca attcaggtcg caagccaggt gggaaaaggc gcaacgttca ccctctggct
    25441 tccggtcact attacgcgta aggacccaca aggatgacgc acgataatat cgatattctg
    25501 gtggtggatg atgacattag ccactgcact attttgcagg ctttgctgcg cggctggggc
    25561 tataacgtcg cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt
    25621 tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg acggcattgc cacgctaaaa
    25681 gagatcaaag cgctaaatcc ggcgattccg gtactgatta tgaccgcata ctccagcgtc
    25741 gagacggcgg tagaggcact gaaaaccggg gcgctggatt atctcatcaa gccgctggat
    25801 ttcgataacc tgcaggcgac gctggaaaag gcgctcgcac atacgcacag tgttgacgct
    25861 gaaacgcctg cggtgtctgc cagccagttc ggtatggtag gtaaaagccc ggcgatgcaa
    25921 cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacagtgct gatccacggc
    25981 gattccggca ccggtaaaga gctggtcgca agggcgattc acgccagtag cgcacggagt
    26041 gaaaaaccac tggtaacgct caactgtgcg gcactcaacg aatccttgct ggaatctgaa
    26101 ttgttcggtc acgaaaaagg ggcgtttacc ggagccgata aacgccggga agggcgcttt
    26161 gttgaggcgg acggcggcac gctgtttctc gatgaaattg gcgatatctc gccgatgatg
    26221 caggttcgtc tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg cagcaaccag
    26281 accatctcgg ttgatgtccg gctgattgcg gcgactcatc gcgatcttgc cgcagaggtg
    26341 aatgccgggc gttttcgcca ggatctctac tatcgcctga acgtggtggc gattgaagtt
    26401 ccgtcgctgc gtcagcggcg ggaagatatt cctctgctgg caaaccattt tcttcagcgc
    26461 tttgccgagc gtaatcgtaa ggcggtaaaa ggttttacgc cccaggcgat ggatctgctg
    26521 attcactacg actggccggg aaatattcgt gaactggaaa acacggtgga acgggcggtg
    26581 gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat tgcgagtacg
    26641 ccgatcccgc tggtacaaag tcaggatatt cagccgctgg tggaagtcga aaaagaggtg
    26701 attctggcgg cgctggagaa aacgggcggc aacaaaaccg aagccgcccg ccagttaggg
    26761 atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagcg
    26821 cgccagccga tatctttccg gcagaagcag tcgtcccaat ggatatcggt cattaaggca
    26881 taagcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtaacgca cagtacgcgc
    26941 ccgccgctgg tcactacctg ctcgtcatcc gctagtttcg tgcccgcgtg gaacactttg
    27001 ccgtctgcca cttcttccag cggcaggccg tggatcacat cgccggtgcg gtaatcgccc
    27061 ggatatccac ccgcagccat caccacgccg agagaagcgc gttcatccca ctcggacgtt
    27121 ttctcgtcca gcttgccttc acaggccgcc aggcagagct caaccagatc ggacttcatg
    27181 cgcagcataa tcggctgggt ttctggatcg ccaaagcggc agttaaattc aataaccttc
    27241 ggattgccct gtttgtcgat catcaggccc gcgtagagaa aaccagtgta ggtgttgcct
    27301 tctgccgcca tgcctttcac ggttggccag atgattcgtt ccatcgtgcg ctgatgaact
    27361 tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc cgcccgtgtt tggtccggta
    27421 tctttatcgc ctacgcgttt gtgatcctgg ctggtagcca tcggcagcac atgctcgccg
    27481 tccaccatca cgataaagct cgcttcttcg ccatcgagga actcttcgat aacgatgcga
    27541 tgacccgcgt cgccaaacgc gttgcccgcc agcatatcgt gaacagctgc ttcagcttct
    27601 tccagcgtca tcgccacgat aacgcctttc ccggctgcca gaccgtcagc tttaatgacg
    27661 attggcgcgc ctttctcacg cagatacgcc agcgcaggtt ctacctcggt gaagttctgg
    27721 tattccgctg tgggaatgtt atggcgggcc agaaagtctt tggtgaatgc tttagaacct
    27781 tccagttgtg cggcacctgc ggttgggccg aagattttca gcccggcggc gcggaaggta
    27841 tcgactacgc ctttcaccag cggcgcttcc gggccgacga tggttagatc gatcttttcg
    27901 ttttgcgcga aatccagcag cgccgggata tcggttacgc cgatagcgac gttttgcagt
    27961 gtgggttcca gcgcagtgcc tgcattaccc ggagcaacaa aaacagtttc aaccagcggc
    28021 gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact
    28081 ttcatctgtc gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac
    28141 cgtgctcgtc ggcggcggca atcacttcgt catcacggat agaaccgcca ggctggatta
    28201 cgcaggtcac acccgcagcg gcggcggcat caataccgtc gcggaacggg aagaacgcgt
    28261 cagaagccat cgaggaacct ttcacttcca ggccttcatc ggccgcttta ataccggcga
    28321 tttttgcgga gtacacgcgg ctcatctggc ccgcgccaat gccgatggtc atattgtttt
    28381 tggcatagac aatggcgttg gatttcacga acttcgccac tttccagcag aacagcgcat
    28441 cacgcagttc ctgttcggtc ggctgacgtt tggtgacgac gcgcagttct tctgcaccga
    28501 ccatgcccag gtcgcgatcc tgaaccagca gaccgccgtt aacacgtttg aaatcaagac
    28561 ccggaacacg ctcgccccac tgaccgcagg tcaggacgcg tacgttctgt ttggcggcgg
    28621 tgattttcag ggcttcttcg ctggcggacg gcgcaataat cacttcaaca aactgacgag
    28681 aaatgatggc ctgcgcggtt tccgcatcca gctcgcggtt aaaggcgata atgccgccga
    28741 atgcggaggt cgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg gaattgctga
    28801 tagccacgcc gcacgggttg gcgtgcttca caatcacaca tgctggctcg gcgaactctt
    28861 tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg gccttaccct
    28921 gaacctgggt tgcggtggca acagaggctt ctttcacgtt ctcttctata tagaaggcag
    28981 cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag ttcaggttca
    29041 gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc gtggtaagcc ggaaccatgc
    29101 tgccgaagta gttggcaatc atgctgtcgt aggcggcggt atgttcaaag gctttgatag
    29161 cgagatcgaa acgggttgca agcagaagcg atccttcgtt ggcatccatc tctttaatga
    29221 tggcgtcgta gtcgctgctc ttcaccacga tggcgacatc tttatggttc ttggcggcag
    29281 agcgcaccat cgtcgggccg ccgatatcga tgttctcaac cgcatcttcc agcgagcaac
    29341 cttcacgggc aacggtctgg gcgaacggat acaggttaac aaccaccata tcgataggct
    29401 ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga atgccaccat
    29461 gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat
    29521 cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggctgtg ccccctgtag
    29581 acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt
    29641 cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta aatcccctgg
    29701 atttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc taacgcccct
    29761 aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga
    29821 atttttctct ttcaatggtg atcacaattt tgacagtggc taccgtggga aaaatacaga
    29881 aattacattg atgattgtgg ataactctgt gcgtaaaaag gtataaagcg gggttttgct
    29941 ggggaatgca gcagtcagtc atttttatgc aatttttcta ttgcgacctg cggagaactc
    30001 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    30061 ggttgaagag aaaaatcctg aaattcaggg ttgactctga aagaggaaag cgtaatatac
    30121 gccacctcgc gacagtgagc tgaaagccgc gtcgca
//
LOCUS       DAZGAP010000038        29867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGAP010000038 DAZGAP010000000
VERSION     DAZGAP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.038"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            320..1012
                     /gene="yieP"
                     /locus_tag="ACHLAM_001454"
     CDS             320..1012
                     /gene="yieP"
                     /locus_tag="ACHLAM_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO7853010.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMTEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            1035..2462
                     /gene="mdtD"
                     /locus_tag="ACHLAM_001455"
     CDS             1035..2462
                     /gene="mdtD"
                     /locus_tag="ACHLAM_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO7853011.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2428..3420)
                     /gene="rbsR"
                     /locus_tag="ACHLAM_001456"
     CDS             complement(2428..3420)
                     /gene="rbsR"
                     /locus_tag="ACHLAM_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO7853012.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLLHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3424..4353)
                     /gene="rbsK"
                     /locus_tag="ACHLAM_001457"
     CDS             complement(3424..4353)
                     /gene="rbsK"
                     /locus_tag="ACHLAM_001457"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO7853013.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4479..5369)
                     /gene="rbsB"
                     /locus_tag="ACHLAM_001458"
     CDS             complement(4479..5369)
                     /gene="rbsB"
                     /locus_tag="ACHLAM_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO7853014.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5394..6359)
                     /gene="rbsC"
                     /locus_tag="ACHLAM_001459"
     CDS             complement(5394..6359)
                     /gene="rbsC"
                     /locus_tag="ACHLAM_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO7853015.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6364..7869)
                     /gene="rbsA"
                     /locus_tag="ACHLAM_001460"
     CDS             complement(6364..7869)
                     /gene="rbsA"
                     /locus_tag="ACHLAM_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO7853016.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7877..8296)
                     /gene="rbsD"
                     /locus_tag="ACHLAM_001461"
     CDS             complement(7877..8296)
                     /gene="rbsD"
                     /locus_tag="ACHLAM_001461"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO7853017.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8463..10331)
                     /gene="kup"
                     /locus_tag="ACHLAM_001462"
     CDS             complement(8463..10331)
                     /gene="kup"
                     /locus_tag="ACHLAM_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO7853018.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10554..12050
                     /gene="ravA"
                     /locus_tag="ACHLAM_001463"
     CDS             10554..12050
                     /gene="ravA"
                     /locus_tag="ACHLAM_001463"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO7853019.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            12044..13495
                     /gene="viaA"
                     /locus_tag="ACHLAM_001464"
     CDS             12044..13495
                     /gene="viaA"
                     /locus_tag="ACHLAM_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO7853020.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCMALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13500..14492)
                     /gene="asnA"
                     /locus_tag="ACHLAM_001465"
     CDS             complement(13500..14492)
                     /gene="asnA"
                     /locus_tag="ACHLAM_001465"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO7853021.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14644..15102
                     /gene="asnC"
                     /locus_tag="ACHLAM_001466"
     CDS             14644..15102
                     /gene="asnC"
                     /locus_tag="ACHLAM_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO7853022.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15192..15635
                     /gene="mioC"
                     /locus_tag="ACHLAM_001467"
     CDS             15192..15635
                     /gene="mioC"
                     /locus_tag="ACHLAM_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO7853023.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            16014..17903
                     /gene="mnmG"
                     /locus_tag="ACHLAM_001468"
     CDS             16014..17903
                     /gene="mnmG"
                     /locus_tag="ACHLAM_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO7853024.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17967..18590
                     /gene="rsmG"
                     /locus_tag="ACHLAM_001469"
     CDS             17967..18590
                     /gene="rsmG"
                     /locus_tag="ACHLAM_001469"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO7853025.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDGERHLVVIKANKI"
     gene            19207..19587
                     /gene="atpI"
                     /locus_tag="ACHLAM_001470"
     CDS             19207..19587
                     /gene="atpI"
                     /locus_tag="ACHLAM_001470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO7853026.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            19596..20411
                     /gene="atpB"
                     /locus_tag="ACHLAM_001471"
     CDS             19596..20411
                     /gene="atpB"
                     /locus_tag="ACHLAM_001471"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO7853027.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            20458..20697
                     /gene="atpE"
                     /locus_tag="ACHLAM_001472"
     CDS             20458..20697
                     /gene="atpE"
                     /locus_tag="ACHLAM_001472"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO7853028.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            20759..21229
                     /gene="atpF"
                     /locus_tag="ACHLAM_001473"
     CDS             20759..21229
                     /gene="atpF"
                     /locus_tag="ACHLAM_001473"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO7853029.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            21244..21777
                     /gene="atpH"
                     /locus_tag="ACHLAM_001474"
     CDS             21244..21777
                     /gene="atpH"
                     /locus_tag="ACHLAM_001474"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO7853030.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            21790..23331
                     /gene="atpA"
                     /locus_tag="ACHLAM_001475"
     CDS             21790..23331
                     /gene="atpA"
                     /locus_tag="ACHLAM_001475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO7853031.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            23382..24245
                     /gene="atpG"
                     /locus_tag="ACHLAM_001476"
     CDS             23382..24245
                     /gene="atpG"
                     /locus_tag="ACHLAM_001476"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO7853032.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            24272..25654
                     /gene="atpD"
                     /locus_tag="ACHLAM_001477"
     CDS             24272..25654
                     /gene="atpD"
                     /locus_tag="ACHLAM_001477"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO7853033.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            25675..26094
                     /gene="atpC"
                     /locus_tag="ACHLAM_001478"
     CDS             25675..26094
                     /gene="atpC"
                     /locus_tag="ACHLAM_001478"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO7853034.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            26443..27813
                     /gene="glmU"
                     /locus_tag="ACHLAM_001479"
     CDS             26443..27813
                     /gene="glmU"
                     /locus_tag="ACHLAM_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO7853035.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQSQKEGWR
                     RPVKKK"
     gene            27975..29804
                     /gene="glmS"
                     /locus_tag="ACHLAM_001480"
     CDS             27975..29804
                     /gene="glmS"
                     /locus_tag="ACHLAM_001480"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO7853036.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         7372 a         7622 c         7675 g         7198 t
ORIGIN      
        1 ctcagcggcg cggggtgtgc ataatacgct ttcccgctac agagtcaagc atttgttttt
       61 gctttctctg ccggaattct caggagaacc ccgctaaccc ggcggcgtgt ttgccgttgt
      121 tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagag gaaatttaaa
      181 ataattttct gaccgcgcat tttttcagca aaagccccgt taatgtgcca taaaacaagc
      241 aatttgccgt ttttgcaacc gtaatcacac ttcctggtgg catactaagg aggaataaaa
      301 agaaaaagga agattagtaa tgcctttaag cgcacaacag ttagctgcac agaaaaacct
      361 ttcgtatgtt ctggctgaga agctggcgca acgcatctta aaaggtgaat atgaacccgg
      421 caccattttg cctggtgaga ttgagctggg cgagcaattt ggagtgagtc gtacagcggt
      481 acgcgaagcg gtcaaaacgt taacggcaaa agggatggtt ttaccgcgac cgcgaattgg
      541 tacccgggtc atgccacaat caaactggaa ttttcttgat caggaattgc ttacctggtg
      601 gatgacagaa gagaactttc atcaggtcat cgatcacttt ctggttatgc gtatctgtct
      661 ggagccgcaa gcttgcctac tggcagcaat ggttggcacc gcggaacaga aggcgcatct
      721 caatacgtta atgaccgaaa tggtggcatt aaaagagaac tttcgccgtg aacgctggat
      781 tgaggtcgat atggcctggc atgagcatat ctatgaaatg agcgccaatc cgtttttgac
      841 ctcattcgcc tcgctcttcc attcggttta tcacacttac ttcacgtcaa ttaccagcga
      901 cacagtgata aagctggatc tgcaccaggc gattgttgac gcgattatcc aaagcgatgg
      961 cgacgcggca tttaaagctt gtcaggcgct gctacgctca cctgataagt gacaccccga
     1021 taacaggaca ttgaatgagc gataaaaaga agcgcagtat ggcgggtttg ccgtggatcg
     1081 cggcgatggc cttcttcatg caggcacttg atgccactat tctgaatacc gccttacccg
     1141 caatcgctca tagccttaat cgttctcctc tcgcgatgca atcagccatc atcagttata
     1201 cgctgacggt ggcgatgctt attccggtaa gcggatggct agccgatcgc ttcggtacgc
     1261 gtcgcatatt tactcttgcc gtgagtctgt tcacgttggg ttctctggcc tgcgcacttt
     1321 ctaactcgct accacagctg gttgtcttcc gggttattca ggggataggc ggcgcaatga
     1381 tgatgcctgt tgctcggctg gccttactgc gcgcttatcc tcgtaatgag cttcttcctg
     1441 tattgaattt tgtcgccatg ccgggtctgg tggggccaat tttaggccct gttcttggcg
     1501 gcgtgctggt cacctgggca acctggcact ggatattttt aatcaatatc cccataggta
     1561 ttgcgggcct tctttacgcg cgcaaacata tgcccaattt caccaccgca cgacgcagat
     1621 tcgatatcac tggctttttg ctgtttggcc ttagccttgt tctcttctca agcggaatag
     1681 agctattcgg ggaaaagatt gtcgccagtt ggattgcctt gacggtaatt gtcaccagca
     1741 tcgggttact gcttctctat attctccatg cacgacgcac gccaaaccca ttaatttcat
     1801 tagatttatt taaaacccgc actttctcga tcggtatcgt aggcaatatt gcaacccgtc
     1861 tggggaccgg ttgtgtaccg ttccttatgc cattgatgtt acaggtagga tttggttatc
     1921 aggcgtttat tgccggctgt atgatggcac cgacagcgtt aggttccatt attgcaaaat
     1981 cgatggttac ccaagtctta cgtcgtctgg gctatcgcca tacattagtg gggatcacgg
     2041 tgattattgg gctaatgatc gctcagttct ctttgcaatc accggcaatg gcgatatgga
     2101 tgctgatctt gccgttgttt atattaggga tggctatgtc gacgcaattt accgcgatga
     2161 ataccatcac acttgccgat ctgaccgatg acaacgccag cagcggtaac agtgttctgg
     2221 cggtcacgca gcaactgtct atcagtttag gcgttgctgt aagtgcggcc gtccttcgcg
     2281 tttatgaagg gatggaaggc acaacgactg tcgaacaatt ccactatacg tttatcacga
     2341 tgggcattat tactgttgct tcagcagcaa tgttcatgct tctgaaaaca accgatggta
     2401 ataatttgat caaaagacag cgtaaatcta agccgaaccg cgttccatca gaatcggagt
     2461 aagttgtaat cgttgttgct gaagggtcgg ttgggttatc cgatggatga gtacatcaat
     2521 cgccagctcc cctagttcat ctttcggttg gtggatagtg gttaatggtg gcgtcataaa
     2581 gcttgccagt tcgatatcgt catagccaat caccgcaata tcctgcggaa cttgtaactc
     2641 tgcctgatac agcgcctggt aaacgccaac agccatagcg tcatttccgg taaagacggc
     2701 ctgaggacgc agcggatgta ataacagttg gcgcatagcg tcaaacccgc cgttaaattc
     2761 aaaatcacca gtgacttcat agccatcagg aatgttgaga cccgcacgtt tcatcgccgc
     2821 ccgataacct tccaaccgca ggcgtgccgg agttttatcc agcgggccgg taatacaggc
     2881 gatacgggta tgacctttat cgatcagata ttgcgttgct aagtctccgc ccagcaacga
     2941 gttatcctga ataagatcgc tgtcgccatc gaacggagcc cagtccatca tcacagtagg
     3001 cactgtcgga taacgttgca tgatttcacg cgaaggttga tgcgtttcgg tgcacagtaa
     3061 cagcaagcca tcaacgcgtt tttgcatcag cgtttccaga ttgcgattca tccgctgttc
     3121 atcgccttcg gtattgcaaa ggacaagact ataaccgcgt tcgaagcaac tgcgttcaac
     3181 gccacgcacc agttcagaat agaaaggatt ggtactggca gtgatcaaca tgccaatggt
     3241 atgtgtttga ttgagtttga ggctacgcgc cagggctgat ggcgcgtaat tgagttcttt
     3301 aatcgccgct tcaactttgg cggtaatcgc ttcactgacg aagcgatctt tattgataac
     3361 gtgagaaact gttgaggtag aaacgcccgc caggcgggca acatctttca ttgtagccaa
     3421 gcgtcacctc tgcctgtcta aaaatgcgtc gatctcttca cgccacggta cggaaggttg
     3481 tgcgccttta cgtgttacgg caatcgcagc ggcagcatga gcaaaccgaa tcgcctctgg
     3541 caatggtttt tcttccagca acgccgtgat taacgcgccg ttaaaggtat ctccggcagc
     3601 aatggtatcg acagcctgca cccggaatcc aggaacgcgc tgaccttcac cattcacgct
     3661 agcccataca ccacgacttc ctaaagtaat cagtacagta cggatacctt tttcatgcag
     3721 tacctgcgcc gccttcgctg catcttcatc attttcaaca cgaataccgg tgagcttttc
     3781 tgcttccgtt tcgtttggcg taataatgtc caccagtgcc agcagttcgt caggaagttc
     3841 gcgagccgga gccgggttaa gcgcaacgat agtcttattt tgatgggcga ttttcgccgc
     3901 tgccatcaca ctttcgagtg gtgattccag ctgcattaat aatgctgacg cgttggcaat
     3961 acgctcacgt tgcgcttcca ccagtgccgg ggaaagggca gcattagcgc cggcatgaat
     4021 accgatgaca ttctcacctt cgccattaac aaaaatcagc gccacacctg ttgattcgcc
     4081 tttgatcacg ctgaccggag aaatatcgat gttatcagtg gcgagctgct ggcgaacgct
     4141 ctcaccaatg ctgtcatcgc cagtgcaggc aataaacgcg atattcgcac cgctacgccc
     4201 agcagccaca gcctgattag cgcctttgcc gccaaatgca acctgatagt ggttaccggt
     4261 tacggtttcg cctggggtag gaaaagattg aagattaaga atgtggtcag cattaatgct
     4321 gccaagaaca acgaggctgc ctgcgttttg catattcggg atgtccataa aatgcgccac
     4381 cgtgttaggg tggcgcgtgc cctgcttttc tttatattac acgttcatct gtcgatgccg
     4441 tatttatgtc accatcaggt catacaacct gattaaaact actgcttaac aaccagtttc
     4501 agatcaaccg gatacttagc ctgaactttc tcacctttca gtactttatc ggcggtttcg
     4561 acgcctttcg cgccaatctg atcgggtagc tgagcgatag tcgctgctag tttgccatca
     4621 ttcaccgctt tttcgccatc cggtgtaccg tcaaatccga cgaccatcac atccgattta
     4681 ccggcagttt gcagtgcgcg cagcgcgccc agcgccattt catcattctg cgcgaataca
     4741 gcctgaacat ccggatgtgc ggtcaacagg ttctgcatta cgttcaaacc tttagtgcga
     4801 tcaaaatctg ctggctggct ggcaagaaca ttaaacttgt gagcagcaac agcctgctgg
     4861 aagccttcgc cacgttcacg ggctgcggat gtaccggcaa tgccttgcag ctcgataact
     4921 ttggcacctt cacccgcttt cttcgcgatg taatcaccgg cgattttgcc gcccagtacg
     4981 ttatcagaag caatgtggct caccacttca cctttcgttg cctggcggtc aagagtgata
     5041 accgggatgt tcgcctggtt agccatcttc acagcattac ccactgcgtc ggagtcggtc
     5101 gggttaatca gcagaatttt ggtgccgcga acggttaagt cctgcacgtt cgccagctct
     5161 ttcgccgggt tattctggga gtccagcacc accaggttat agccaagttt atccgcctct
     5221 ttctgcgcgc catctttcag cgatacaaag aacgggttgt taagcgtgga gaccaccagc
     5281 gcgatggtgt cttttgccat cgcattcgca ctgacggtgg cgcttagcgc aacagcggaa
     5341 accagggtag ccagtttttt catgttcata ttcaagatgt cctgtagtcg ttattactgc
     5401 tttttgttgt ctaccagcac cgccagcaaa atcaccaccg ctttgacgat catctggtaa
     5461 taggaggaaa cacctaacaa attcaatcca ttattaagga agccaagaat taatgcgccg
     5521 atcaacgtcc caacaatgcg accttttccg cccgccagac tcgtaccgcc cagaaccacc
     5581 gcagcaatgg catccagctc atagccagtc cccgccgtag gttgtgcgga ggagagacgc
     5641 gccacttcaa tgatcccggc cagcgatgcc agcagaccac aaagagaata gacgatgatt
     5701 ttgattttat tgacgttgat accagaaagg cgcgttgccg cttcgttgcc gcccagcgcg
     5761 tagatgtaac gccccagacg cgtgtgatgc agcatgtacc aggccgcgag gaagacaatt
     5821 cccatgatcc agactggcgt cggtacaccc agcggacgac caataccaaa ccagccaaac
     5881 agatcggcgt tctcagtaaa gccagtattc actgggctac cgttggtata aaccatggtc
     5941 acgccgcgca gtaaaagcat cataaccagc gtagcgataa aggcctggac gcgacctttc
     6001 gctacaatca ccccggttac cgcaccaatt gcggcaccta acgcgagagc ggcagcgaca
     6061 gccaccagcg cattgacttc aatgccaacg atagatgcag caactgcgcc ggtcagcgcc
     6121 aatagagaac ctaccgacaa gtcgatgccc gacgtcagga tcaccagcgt catcccgact
     6181 gccataatgg cgttcaccga ggtttgctgg agaatattga ataagttatt gatggtgaaa
     6241 aagttcgggc ttaacgtcga gacaatcgcg atcagcacca gcagagcgat aagcgatttc
     6301 tgctccatca gccacgcttt cgtgaaataa cggcgaccag agacagtctg ggttgtcatt
     6361 tttttactcc tgattcacgc gattaagctt gcccacagcg gcagccatta acacttcctg
     6421 ggtggcctgc tcacgagtaa attccccgct gagatgccct tcatgcatga cgatgatgcg
     6481 atcgctcatg cctaatactt ctggcatctc cgatgacacc agaatgatgc tcaagccatc
     6541 ggctttgaac tggttaatca gttgatagat ctcttttttc gcgccgacat ctacgccacg
     6601 ggtaggctca tcgaggatca acactttggg gcgtgtcatc agaccacggg caatcgccac
     6661 tttttgctga ttgccaccgg agagcagacc aattgcctgt tccatcgacg gcgttttcac
     6721 attaaacaga cgaatgaaat cacttactgc ctgctgttca tcggcatgct tcaaactgcc
     6781 gccagcgcgg ctgaagtagc gcagcgctgt cagcgacatg ttctctttta ctgacatgcc
     6841 cagcactaaa ccgtcacgtt tacggtcttc ggagatatac acaatgccgt ttgccaggcc
     6901 atcctgcggt gaacgggtaa cgacttcatg cccatccagg gtgacgtaac cgctggtgcg
     6961 cggcagtgcg ccgtagagca ctttcatcag ttcggtacga cctgcgccca tcaaaccaga
     7021 gacgccaaga atttcgcctt tgcgtaaagt aaaagagaca tcgttaacgc caggtccgca
     7081 gagattatcg actttcaggc ggatatctcc cggcgctttg tccaggtgcg gatattgatc
     7141 ttccagcttg cgacccacca tcatctcaat cagcgaatct tcggtcagtg atgctacttc
     7201 acgctcagca ataaattgcc catcacgaaa aacggtaacg tcatcgcaaa tctcgaagat
     7261 ttctttcatg cggtgggaga tatagacaat accgcggcct tgcgatttca gctcgcggat
     7321 gacgcggaac aaggattcgg tttcggtatc ggtcagcgca tcggtcggtt catccataat
     7381 gatgactttc gactcaaagc tcagcacttt ggcaatttca accatttgct ggtcaccgat
     7441 ggaaagatcg cccaccagct tgtcgctttt aaagcgcagg ttaagtttag ccagcaattt
     7501 atccgcttcg gcatacatgg ttttccagtc aattttgcca aagcgattaa caaactcacg
     7561 accgaggaaa atgttttcgg caatggtcaa ctgcgggatc aggttcagtt cctgatggat
     7621 aatcccaatc ccggcttcct gggaagattt cggcccggta aacgtcgttt ctttccccag
     7681 ccataaaagc gtaccggcat cgcgagtata gatacccgta agcactttca tcatggtgga
     7741 tttacccgca ccgttttcgc ccaccagcgc catcacgcgg ccaggataga catttaacgc
     7801 tgcgcccgag agggctttta cgcccgggaa ggctttatcg atgcctttaa gctgaagtaa
     7861 tgcttccatg acggcctcag aacgtcacgc cagcacagag aatgatattc gcatacggag
     7921 aacattctcc gctgcgaatt accgcctgac tttctgcggt ttgttgtttg aattgttcat
     7981 gcgtggtgta acgaatttca atggtatttc cctggtgttt ttgcagctgc tcaaggtgag
     8041 tgagcaacgt ttcgtggaga tgcggattat gttgtttgat ctcttccgcg ataatggctg
     8101 cctcgacctg catttcattt gtgacgacgc ccagcacctg cataaaagaa ggtacgccct
     8161 gggttaatgc catatcgata cgcgttgtac ttttggggat gggtaaacca gcatcacaca
     8221 ccaccagcgt atcggtatgt cccagacggg agatcaccga tgaaatatca gaattaagaa
     8281 cggtgccttt tttcattttt ttctccatca gcgaaacgtt tcgctgaccc gcagtttaat
     8341 cctgactgag ttgggaaaac catcgtgacg ttacggaaat gtgatcaacc tcgaaacgtt
     8401 ttacatggta attaaccatg aaaacagaaa cgccccctta aggagaaagg aggcgtctgg
     8461 cgttagattt cgacctgagt acccagttcg ataaccctgt ttggcgggat ttcaaattga
     8521 tccggcgcac gcagcgcatt acgttgcagc agcaagtaca gcttgccgcg cagacgcaaa
     8581 taccacgggc gtttgccgag gatcaacgac tcatgcgaca taaagaagga ggtttccatc
     8641 atccggcaac ttaatccttc cagaccgcag cggtggaaaa cttcttccac gtttggcgtt
     8701 tctcgccaac cataacttgc caccacgcgc cagaaagtgg gcgacagttg ttcaatctgt
     8761 acccgacgga cgttatggac atatggcgcg tcttcggtgc gcagagttaa cagaatcacc
     8821 cgctcatgca ataccttgtt atgtttaagg ttatgcatca gcgcaaaggg aatgacgttg
     8881 attgcacgtg acatatacac cgcggtcccg ggcacgcgaa cgggcggtga tttctccagc
     8941 gaagcaatca tcgcctccag agagttacca tgttcatgca tccgccgcag caaacggaaa
     9001 cgttcgctct tccaggtggt catcacgata aacatcaccg taccgaggct caatggcaac
     9061 cagccgccgg agagcagttt atcgaggtta gcggtgaaca atggaatatc gacacaaagg
     9121 aaagcaatca ggatcagcgc aacaaaatac ttattccagt gccagttctg acgtgccacg
     9181 gtagtcgaga gaatagacgt cagcaccatg gttccggtca ccgcaatccc gtacgccgcc
     9241 gccaggttgc tggagtgctc aaagctgaca atcacaatca cgaccgcgac atagagcatc
     9301 cagttcacaa agggaatata gatttgccct gactccattt cggaggtgtg aataatgcgc
     9361 atcggcgaca aatatcccag acgtaccgcc tgacgcgtca atgagaagac gccagagata
     9421 acagcctgcg aggcaattac cgtcgccagt gcggcgatga tcagcagcgg gatcagcgcc
     9481 cagtccggtg ccaacaggaa gaacgggttc ttaatcgctt ccgggttctt taacaacaac
     9541 gctccctgac cgaagtaatt aagggtcaag gaaggtaata cgacggtgaa ccacgccagg
     9601 cgaataggga acttaccaaa gtgccccata tcagcataca gcgcctcgac ccccgtaatc
     9661 gacagcacca ctgcccctaa tgcaataaaa gaaaccgttt tgtattcaag gaagaaatgc
     9721 accgcccaca tcggattcag cgcatgcagc acttccgggt tagcaataat gctacgtaac
     9781 cccagtcctg ccagaatcaa aaaccaggtc agcatgatcg gcgcaaacag cttaccgacc
     9841 atagccgtgc catgcttttg aatcataaat aataaagtga gaacgataat tgagagggga
     9901 actatccagg tatccagctg cggggcgacg atttccagac cttcaatggc ggacatcacc
     9961 gaaatagcgg gtgttatgac gacttcacca tagaaaaagc tgccgccgat tagccccata
    10021 atcaccagca ttgatgtggt tcgcgccgac gtattacgcc cggcaagcga catcaacgtc
    10081 aggatccccc cttcaccggc gttatctgcc cgcatcacga aggtgagata tttaatagaa
    10141 accacaaaga ttagcagcca gaagatcagc gataaaaagc caaacacggc atcgcgctca
    10201 acgccaaaac caaactggcc ggacaaacat tcacgaagtg tatataacgg gctggtacca
    10261 atatcgccgt agacaactcc aatcgccgcg agggtaatcg cgggcaatga ttgcttatta
    10321 tcagtgctca tagactagtc tttcgttgaa atatgaaatg tgtgcttagt cccttggccc
    10381 acaaaaaagc gcacagtatg cacgattaac ggcaaaatcg tactcctaaa tgcgggcaca
    10441 ttaacctggc tcaaagaaaa ataccccccc tttgagacta ttttagccac cagcaagaaa
    10501 cgtctatact cgcaatttac gcagaacttt tgacgaaagg acgccacttc attatggctc
    10561 accctcattt attagcggaa agaatttccc gcctgagcag ttcgttggaa aaggggcttt
    10621 atgaacgtag ccacgccatc cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt
    10681 tcctccttgg cccgccaggt attgccaaaa gtttgatcgc ccggcgctta aaattcgcct
    10741 ttcagaatgc ccgcgcgttt gaatatctga tgactcgctt ctccacgccg gaagaggttt
    10801 ttggtcccct ttctattcag gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg
    10861 gttacctgcc ggaagcagaa atcgtctttc tggatgagat ctggaaagcg ggtccggcaa
    10921 ttcttaatac cctgctcacc gccattaacg agcgccagtt ccgcaacggt gcacacgtag
    10981 aaaaaatccc gatgcgcctg ctggtggcgg cctccaacga gctgccggaa gcagacagca
    11041 gtctggaagc gttatatgac cgcatgctga ttcgtctgtg gttagataaa gtgcaggata
    11101 aagcgaattt ccgctccatg ctgaccagtc aacaggatga aaacgacaat ccggttcctg
    11161 cctccttgca ggtcacagat gaagaatatg aacgctggca gaaagagatt ggtgaaatta
    11221 cgctgcccga tcatgtattt gagctgattt ttatgctgcg ccagcaactg gataaattac
    11281 cggatgcgcc ttatgtctcg gatcgtcgct ggaaaaaagc gattcgatta ttgcaggcca
    11341 gcgccttttt tagcggtcgc agtgctgttg ccccggttga tctcattttg ctgaaagatt
    11401 gcctgtggta tgacgcgcaa agcctgaatt tgatacaaca acaaattgat gtattgatga
    11461 ccggtcatgc ctggcaacag caaggaatgt tgacccgcct gggcgcgatt gtgcaacgtc
    11521 acctgcaact ccagcagcaa caaagcgata aaacagcctt aacggtaatt cgtctgggcg
    11581 gcattttcag ccgtcgtcag cagtatcaac ttcctgttaa cgttactgct tccactctga
    11641 ctctgctgct gcaaaaaccg ttaaaactgc atgatatgga agtggttcat atctcctttg
    11701 agcgtaacgc gctggaacag tggctgagta aaggtggtga aattcgcggc aaactgaacg
    11761 gtatcggctt tgcccagaaa ttgaatctgg aagtggatag cgcccaacat cttgttgtac
    11821 gcgatgtaag tttacaaggc agtacgctgg cacttcccgg ttcatcggct gaaggtctgc
    11881 caggtgaaat aaaacaacaa ctggaagagc ttgaaagcga ctggcgcaag caacacgctt
    11941 tattcagcga acagcaaaaa tgtctgttta tccctggcga ctggttaggt cgcattgaag
    12001 ccagcctaca ggatgtcggc gcccagattc gccaggcaca acaatgctaa cgctggatac
    12061 gcttaatgtg atgctggccg tcagcgaaga gggattgatc gaagagatga tcatcgcgct
    12121 gctggcctca ccgcagctgg cggtcttctt tgaaaaattc ccacgactga aggcggcaat
    12181 cactgatgat gttccccgct ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt
    12241 cccgccggaa ctcaccgaag aggtgatgtg ctatcagcaa agccagctcc tctccacgcc
    12301 gcagtttatt gtgcagctac cacagatcct ggacttactg catcgtctga attctccatg
    12361 ggcagaacaa gcccgacagt tggttgatgc taacagtacg atcacttcag cgttacacac
    12421 gctttttctc cagcgctggc gtttaagtct gatcgtgcaa gcaacgacgc taaatcagca
    12481 gctattagaa gaagaacgcg aacaactgtt gagtgaagtt caggaacgca tgacgctgag
    12541 cgggcaactt gaaccgattc tcgcagataa caataccgca gctggtcgtc tgtgggatat
    12601 gagcgccggt cagcttaaac gcggcgacta tcagttgatt gtgaaatacg gtgaatttct
    12661 taacgaacag ccggaactga aacgcctggc agaacagttg gggcgttcac gggaagccaa
    12721 atcaataccg cgcaacgatg cgcagatgga aaccttccgc accatggtgc gcgaaccggc
    12781 gacggttcct gagcaggttg atggtctgca acaaagcgat gatattttac gtctcctgcc
    12841 gccagaactg gcgacactgg ggataacgga actggagtat gagttttacc gtcggctggt
    12901 ggaaaaacag ttgctcacct atcgcctgca cggtgagtcg tggcgtgaaa aagtgatcga
    12961 acgcccggtg gtacataaag attacgacga acagccgcgc ggaccgttta ttgtctgtgt
    13021 ggatacttcc ggctcaatgg gcggctttaa tgaacagtgt gcgaaagcgt tctgcatggc
    13081 cttgatgcgc attgctctcg ctgaaaaccg gcgctgctat attatgttat tttccaccga
    13141 gatcgtccgt tatgagcttt caggcccaca aggcatcgaa caagcaatcc gttttttaag
    13201 ccagcagttt cgtggcggca ccgatcttgc cagttgtttt cgcgccatta tggaacgctt
    13261 gcaaagcagg gaatggtttg atgccgatgc ggtggtgatt tctgatttta tcgcccagcg
    13321 gttgcctgac gacgtgacga gtaaagtgaa agagctacag cgcgtacatc agcatcgctt
    13381 tcatgccgtg gcgatgtctg cacatggcaa acccggcatc atgcgcattt tcgatcatat
    13441 ctggcgcttt gataccggga tgcgaagccg cctgctcaga cgctggcggc gataaattat
    13501 tacagcagag aagggacgct ctcgcgaaca gctgccggcc atacaccaca ctgaacctgg
    13561 ccgatatgtg gcagttgcag cagcagcatg gtcaaacgag actgcccgat accgccgccg
    13621 atggtctgcg gcatttcacc gcgcagcagc gcctgatgcc actccagctc caggcgatct
    13681 tcatcaccgg tcagcgccag ctgatgtttc aacgtatcgg catccacgcg gatccccatg
    13741 gaagaaagct caaacgcatc ttccagtacc gggttccata ccagaatatc gccgttcaga
    13801 cccgcatggc ccagctctga cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg
    13861 tgacgatgac catcactcaa cttgccgcca atcccgacga ggaataccgc gccaagatct
    13921 ttcgcaatcg cccgctcacg ccctttggca tcgagatccg gataacgaga cagtaactcc
    13981 tggctgtgta cgaagtggat ctgatccggc aggaacggtg ccaggccaaa ctcttcgcta
    14041 accgcagctt cggttgcttt aattcccgcc cagatcgcct ctaccgtgct tttcagagtc
    14101 gagaattgac gctcaccgtc gcccattacg cgttcccagt cccactggtc aacatagacc
    14161 gagtgcaacg gagaaagacg gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc
    14221 ccttcgcccg cgctgaagtc gtgttgccct aatgtctgac gtttccactt cgccagtgaa
    14281 tgaaccactt cgaactgggc atcaggcaga gctttcactt ttacctgcac cgctttttca
    14341 cagcccgaca agttatcctg cgtgccatcc cccacacggc taagaatcgg cgcctggact
    14401 tcgatcagcc ccagacgttc ttccagttga cgggaaaagt gagatttcac gaagctaatt
    14461 tgacgttgtt tggcaatgta agcggttttc attttttata ctcctgcgtc ctgttgctta
    14521 tgattaagca acaaaaaccg acacgcatgc aataatcatt caataaaaag cctaactcac
    14581 ttttgattca ttaaaaaaga aggctaaaat agaatgaatc atcaatccgc ataagaaaat
    14641 cctatggaaa attatctaat cgacaatctg gaccgtggca tcctggaagc attaatgggc
    14701 aatgcgcgca ccgcttacgc cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt
    14761 cacgttcgag tagagaaaat gaagcaggcg gggatcatta ccggggcgcg tattgatgtc
    14821 agcccgaagc agctcggtta tgacgtaggc tgctttatcg gcattatatt aaagagcgcc
    14881 aaagactatc cttccgcgct ggcgaaactg gaaagccttg atgaagtcac tgaagcctat
    14941 tacaccaccg gccactacag catctttata aaagtgatgt gccgttcgat cgacgctctc
    15001 cagcatgtac ttatcaacaa gatccaaaca attgatgaaa ttcagtccac cgagacattg
    15061 atcgtcctgc aaaacccgat catgcgtacc atcaagccct gatcggcttt tttaatccca
    15121 tacttttcca caggtagatc ccaacgcgtt cacagcgtac aatacgccac tcttaataaa
    15181 ggtggcggtt tatggcagat atcactctta tcagcggcag caccctcggc ggtgccgaat
    15241 atgtagcaga acacctggct gaaaagctgg aagaggcggg ttttaccacc gaaacgctgc
    15301 acggtccgct gttggaagat ttatctgcct ctgggatctg gctggttatc agctcaaccc
    15361 acggtgccgg agatattccg gacaaccttt ctcctttcta tgaagcattg caggaacaga
    15421 agcccgatct ttctgcggtc cgctttggcg caatcggtat tggtagtcgt gaatatgaca
    15481 ccttttgtgg ggctatcgat aaactcgagg cagaactcaa aaattccggt gcaaaacaga
    15541 caggcgaaac actgaagatc aacattcttg atcacgacat tccggaagat ccggcagaag
    15601 aatggctggg atcgtggatt aatttactca aataagtata cagatcgtgc gatctactgt
    15661 ggataactct gtcaggaagc ttggatcaac cggtagttat ccaaagaaca accgttgttc
    15721 agtttttgag ttgtgtataa cccctcattc tgatcccagc ttatacggtc caggatcacc
    15781 gatcattcac agttaatgat cctttccagg ttgttgatct taaaagccgg atccttgtta
    15841 tccacagggc agtgcgatcc taataagaga tcacaataga acagatctct aaataaatag
    15901 atcttctttt taatacccag gatcccaggt ctttctcaag tcgacaaagt tgagtagaat
    15961 ccacggcccg ggcttcaatc cattttcata ccgcgttatg cgaggcaatc accatgtttt
    16021 atccggatcc ttttgacgtc atcatcattg gcgggggtca tgcaggcacc gaggccgcga
    16081 tggccgcggc gcgtatgggt caacagactc tgcttttgac acacaatatc gacactctgg
    16141 ggcagatgag ctgcaacccg gcgatcggcg gtattgggaa gggacatctg gtaaaagaag
    16201 tggatgcact cggcggtctg atggcgaaag cgatcgatca ggcgggtatc cagtttagga
    16261 tactaaacgc aagcaaggga ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc
    16321 tctaccgtca ggcggtacgt acggcgctgg agaaccaacc gaacctgatg atcttccagc
    16381 aggcggttga agatcttatt gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg
    16441 gactgaagtt ccgtgccaaa gccgtcgtgc ttaccgttgg gacgttcctc gacggtaaaa
    16501 ttcatatcgg tctggataac tacagcggtg gtcgtgctgg tgatccgccg tccattccgc
    16561 tctctcgccg tttgcgtgaa ctgccgctgc gcgttggtcg tctgaaaacc gggacaccgc
    16621 cgcgtattga tgcgcgtact atcgacttca gcgtgctggc gcaacagcat ggcgataacc
    16681 caatgccggt attctcgttt atgggtaatg cgtcccagca tccacagcag gtgccgtgtt
    16741 atatcaccca taccaacgag aaaacccatg atgtgatccg cagtaacctc gatcgtagcc
    16801 caatgtatgc aggggtgatc gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca
    16861 aagtcatgcg cttcgccgac agaaatcagc atcagatctt ccttgaaccg gaagggctga
    16921 cctcaaacga aatttatccg aacggtatct ccaccagcct gccgttcgat gtgcagatgc
    16981 aaatcgtccg ctccatgcag gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca
    17041 ttgagtatga cttcttcgat ccacgcgacc tgaaaccgac gctggagagc aagtttatcc
    17101 aggggctgtt ctttgctggt cagattaacg gcactaccgg ttacgaagaa gccgctgcgc
    17161 aaggtttgct ggccggtctt aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc
    17221 cggcgcgttc tcaggcgtat ctcggcgtac tggttgatga cctgtgcact ttaggaacca
    17281 aagaaccgta tcgtatgttt acctcgcgcg cagaatatcg tctgatgctg cgcgaagata
    17341 atgcggatct gcgtttgact gaaattggtc gtgaactggg cctggtggat gacgaacgtt
    17401 gggcgcgctt taacgagaaa cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga
    17461 cctgggtaac cccgtcggcg gaagctgcag ccgaagtgaa tgctcacctg actgcgccac
    17521 tttcccgtga agccagtggt gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat
    17581 taaccacgct gacgccgttt gcccctgcgt tgacagacga acaggcggcg gaacaggttg
    17641 agattcaggt taaatacgaa ggttatatcg cgcgccagca agatgagatc gaaaagcagc
    17701 tgcgtaacga gaacaccctg ctaccagcga cgctggatta ccgccaggta tccggtcttt
    17761 ctaacgaagt gatcgccaaa cttaacgatc acaaaccggc ctctatcggc caggcttcgc
    17821 gtatttctgg cgtcacgcct gcggccatct ccattctgct ggtgtggctg aaaaaacagg
    17881 gtatgctgcg tcgtagcgca taacgcatta aaaatgcctg gtaagcaccc gcttaccggg
    17941 caacgcatca agaacaggta atcaccgtgc tcaacaaact ctccttactg ctgaaagacg
    18001 caggtatttc gcttaccgat caccagaaaa accagcttat tgcctatgtg aatatgctgc
    18061 ataaatggaa taaagcgtac aacctgactt cggtccgcga tcctaatgag atgctggtac
    18121 gccatattct cgatagcatt gtggtggcac cgtatctgca aggtgaacgg tttatcgatg
    18181 tcggcaccgg tccaggactg cctggcattc cactctctat cgtgcgtcct gaagcccatt
    18241 tcactctgtt ggatagcctt ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc
    18301 ttaaactgga gaatattgag ccagtacaga gcagggtaga agagtttcct tcagagccgc
    18361 catttgatgg cgtaattagc cgcgcttttg cctctctgaa cgatatggtg agctggtgtc
    18421 accatcttcc tggtgagcaa ggccgtttct acgcgctgaa aggccaaatg ccggaagatg
    18481 aaatcgcttt gttgcccgaa gaatatcagg tcgaatcagt ggttaaactt cacgttccgg
    18541 ccctggatgg cgaacgtcat ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa
    18601 aaaaatcata aaaaattgac cggttagact gttaacaaca accaggtttt ctactgatat
    18661 aactggttac atttaacgcc acgttcactc ttttgcatca acaagataac gtggcttttt
    18721 ttggtaagca gaaaataagt cataagtgaa aatatcagtc tgctaaaaat cggcgctaag
    18781 aatcatcatt ggctgttaaa acattattaa aaatgtcaat gggtggtttt tgttgtgtaa
    18841 atgtcattta ttaaaacagt atctgttttt agactgaaat atcataaact tgcaaaggca
    18901 tcatttgcca agtaaataaa tatgctgtgc gcgaacatgc tcaatatgtg atctgaagca
    18961 cgctttatca ccagtgttta cgcgttattt acagtttttc atgatcgaac agggtttgca
    19021 gaaaagtcgc aattgtatgc actggaaaaa tatttaaaca tttattcacc ttttggctac
    19081 ttattgtttg aaatcacggg ggcgcaccgt ataatttgac cgctttttga tgcttgactc
    19141 taagccttaa agaaagtttt atacgacacg cggcatacct cgaagggagc aggagtgaaa
    19201 aacgtgatgt ctgtgtcgct cgtgagtcga aacgttgctc ggaagcttct gctcgttcag
    19261 ttactggtgg tgatagcaag tggattgctg ttcagcctca aagacccctt ctggggcgtc
    19321 tctgcaataa gcgggggcct ggcagttttt ctgcctaacg ttttgtttat gatatttgcc
    19381 tggcgtcacc aggcgcatac accagcgaaa ggccgggtgg cctggacatt cgcatttggc
    19441 gaagctttca aagttctggc gatgttggtg ttactggtgg tggcgttggc ggttttaaag
    19501 gcggtattct tgccgctgat cgttacgtgg gttttggtgc tggtggttca gatactggca
    19561 ccggctgtaa ttaacaacaa agggtaaaag gcatcatggc ttcagaaaat atgacgccgc
    19621 aggattacat aggacaccac ctgaacaacc ttcagctgga cctgcgtaca ttctcgctgg
    19681 tggatccaca taacccccca gccaccttct ggacaatcaa tattgactcc atgttcttct
    19741 cggtggtgct gggtctgttg ttcctggttt tattccgtag cgtagccaaa aaggcgacca
    19801 gcggtgtgcc aggtaagttt cagaccgcga ttgagctggt gatcggcttt gttaatggta
    19861 gcgtgaaaga catgtaccat ggcaaaagca agctgattgc tccgctggcc ctgacgatct
    19921 tcgtctgggt attcctgatg aacttgatgg atttactgcc tatcgacctg ctgccgtaca
    19981 ttgctgaaca tgtactgggt ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg
    20041 taacgctgtc tatggcactg ggtgtattta tcctgattct gttctacagc atcaaaatga
    20101 aaggcatcgg cggcttcacg aaagagttga cgctgcagcc gttcaatcac tgggcgttca
    20161 ttcctgtcaa cttaatcctt gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt
    20221 tgcgactgtt cggtaacatg tatgccggtg agctgatttt cattctgatt gctggtctgt
    20281 tgccgtggtg gtcacagtgg attctgaatg tgccgtgggc cattttccac atcctgatca
    20341 ttacgctgca agccttcatc ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg
    20401 aagaacatta atttaccaac actactacgt tttaactgaa acaaactgga gactgtcatg
    20461 gaaaacctga atatggatct gctgtacatg gctgccgctg tgatgatggg tctggcggca
    20521 atcggtgctg cgatcggtat cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt
    20581 caacctgatc tgattcctct gctgcgtact cagttcttta tcgttatggg tctggtggat
    20641 gctatcccga tgatcgctgt aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa
    20701 gcgttgcttt tatttaaaga gcaatatcag aacgttaact aaatagaggc attgtgctgt
    20761 gaatcttaac gcaacaatcc tcggccaggc catcgcgttt gtcctgttcg ttctgttctg
    20821 catgaagtac gtatggccgc cattaatggc agccatcgaa aaacgtcaaa aagaaattgc
    20881 tgacggcctt gcttccgcag aacgagcaca taaggacctt gaccttgcaa aggccagcgc
    20941 gaccgaccag ctgaaaaaag cgaaagcgga agcccaggta atcatcgagc aggctaacaa
    21001 acgccgctcg cagattctgg acgaagcgaa agctgaggca gaacaggaac gtactaaaat
    21061 cgtggcccag gcgcaggcgg aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg
    21121 taagcaagtt gctatcctgg ctgttgctgg cgccgagaag atcatcgaac gttccgtgga
    21181 tgaagctgct aacagcgaca tcgtggataa acttgtcgct gaactgtaag gagggagggg
    21241 ctgatgtctg aatttattac ggtagctcgc ccctacgcca aagcagcttt tgactttgcc
    21301 gtcgaacacc aaagtgtaga acgctggcag gacatgctgg cgtttgccgc cgaggtaacc
    21361 aaaaacgaac aaatggcaga gcttctctct ggcgcgcttg cgccagaaac gctcgccgag
    21421 tcgtttatcg cagtttgtgg tgagcaactg gacgaaaacg gtcagaacct gattcgggtt
    21481 atggctgaaa atggtcgtct taacgcgctc ccggatgttc tggagcagtt tattcacctg
    21541 cgtgccgtga gtgaggctac cgctgaggta gacgtcattt ccgctgccgc actgagtgaa
    21601 caacagctcg cgaaaatttc tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg
    21661 aattgcaaaa tcgataagtc tgtaatggca ggcgttatca tccgagcggg tgatatggtc
    21721 attgatggca gcgtacgcgg tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg
    21781 gactggagca tgcaactgaa ttccaccgaa atcagcgaac tgatcaagca gcgcattgct
    21841 cagttcaatg ttgtgagtga agctcacaac gaaggtacta ttgtttctgt aagtgacggt
    21901 gttatccgca ttcacggcct ggccgattgt atgcagggtg aaatgatctc cctgccgggt
    21961 aaccgttacg ctatcgcact gaacctcgag cgcgactctg taggtgcggt tgttatgggt
    22021 ccgtacgctg accttgccga aggcatgaaa gttaagtgta ctggccgtat cctggaagtt
    22081 ccggttggcc gtggcctgct gggccgtgtg gttaacactc tgggtgcacc aatcgacggt
    22141 aaaggtccgc tggatcacga cggcttctct gctgtagaag caatcgctcc gggcgttatc
    22201 gaacgtcagt ccgtagatca gccggtacag accggttata aagccgttga ctccatgatc
    22261 ccaatcggtc gtggtcagcg tgaattgatc atcggtgacc gtcagaccgg taaaaccgca
    22321 ctggctatcg atgccatcat caaccagcgc gattccggta tcaaatgtat ctatgtcgct
    22381 atcggccaga aagcgtccac catttctaac gtggtacgta aactggaaga gcacggcgca
    22441 ctggctaaca ccatcgttgt ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg
    22501 gcaccgtacg ctggttgtgc aatgggcgaa tacttccgtg accgcggtga agatgcgctg
    22561 atcatttacg atgacctgtc taaacaggct gttgcttacc gtcagatctc cctgctgctc
    22621 cgtcgtccgc caggacgtga agcattcccg ggcgacgtat tctacctcca ctctcgtctg
    22681 ctggagcgtg ctgcgcgtgt taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg
    22741 aaagggaaaa ccggttctct gactgcgctg ccgattatcg aaactcaggc gggtgacgtt
    22801 tctgcgttcg ttccaaccaa cgtaatctcc attaccgatg gtcagatctt cctggaaacc
    22861 aacctgttca acgccggtat tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt
    22921 ggtggtgcag cacagaccaa gatcatgaaa aaactgtccg gtggtatccg taccgctctg
    22981 gcacagtatc gtgaactggc agcgttctct cagtttgcat ccgaccttga cgatgcaaca
    23041 cgtaagcagc ttgaccacgg tcagaaagtg accgaactgc tgaagcagaa acagtatgcg
    23101 ccgatgtctg ttgcgcaaca gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg
    23161 gatgtagaac tgtcgaaaat cggcagcttc gaagccgctc tgctggctta cgtcgaccgt
    23221 gatcacgctc cgttgatgca agagatcaac cagaccggtg gctacaacga cgaaatcgaa
    23281 ggcaagctga aaggcatcct cgattccttc aaagcaaccc aatcctggta acgtctggcg
    23341 gcttgcctta gggcaggccg caaggcattg aggagaagct catggccggc gcaaaagaga
    23401 tacgtagtaa gatcgcaagc gtccagaaca cgcaaaagat cactaaagcg atggagatgg
    23461 tcgccgcttc caaaatgcgt aaatcgcagg atcgcatggc ggccagccgt ccttatgcag
    23521 aaaccatgcg caaagtgatt ggtcaccttg cacacggtaa tctggaatat aagcaccctt
    23581 acctggaaga ccgcgacgtt aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt
    23641 tgtgcggtgg tttgaacatt aacctgttca aaaaactgct ggcggaaatg aagacctgga
    23701 ccgacaaagg cgttcaatgc gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca
    23761 actccgtggg cggcaatgtt gttgcccagg tcaccggcat gggggataac ccttccctgt
    23821 ccgaactgat cggtccggta aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca
    23881 agctttacat tgtcagcaac aaatttatta acaccatgtc tcaggttccg accatcagcc
    23941 agctgctgcc gttaccggca tcagatgatg atgatctgaa acataaatcc tgggattacc
    24001 tgtacgaacc cgatccgaag gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc
    24061 aggtttatca gggcgtggtt gaaaacctgg ccagcgagca ggccgcccgt atggtggcga
    24121 tgaaagccgc gaccgacaat ggcggcagcc tgattaaaga gctgcagttg gtatacaaca
    24181 aagctcgtca ggccagcatt actcaggaac tcaccgagat cgtctcgggg gccgccgcgg
    24241 tttaaacagg ttatttcgta gaggatttaa gatggctact ggaaagattg tccaggtaat
    24301 cggcgccgta gttgacgtcg aattccctca ggatgccgta ccgcgcgtgt acgatgctct
    24361 tgaggtgcaa aatggtaatg agcgtctggt gctggaagtt cagcagcagc tcggcggcgg
    24421 tatcgtacgt accatcgcaa tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa
    24481 agacctcgaa cacccgatcg aagtcccggt aggtaaagcg actctgggcc gtatcatgaa
    24541 cgtactgggt gaaccggtcg acatgaaagg cgagatcggt gaagaagagc gttgggcgat
    24601 tcaccgcgca gcaccttcct acgaagagct gtcaaactct caggaactgc tggaaaccgg
    24661 tatcaaagtt atcgacctga tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg
    24721 tggtgcgggt gtaggtaaaa ccgtaaacat gatggagctt attcgtaaca tcgcgatcga
    24781 gcactccggt tactctgtgt ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt
    24841 ctaccacgaa atgaccgact ccaacgttat cgacaaagta tccctggtgt atggccagat
    24901 gaacgagccg ccgggaaacc gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa
    24961 attccgtgac gaaggtcgtg acgttctgct gttcgttgac aacatctatc gttacaccct
    25021 ggccggtacg gaagtatccg cactgctggg ccgtatgcct tcagcggtag gttatcagcc
    25081 gaccctggcg gaagagatgg gcgttctgca ggaacgtatc acctccacca aaaccggttc
    25141 tatcacctcc gtacaggcgg tatacgtacc tgcggatgac ttgactgacc cgtctccggc
    25201 aaccaccttt gcgcaccttg acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg
    25261 tatctacccg gccgttgacc cgctggactc caccagccgt cagctggacc cgctggtggt
    25321 tggtcaggaa cactacgaca ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga
    25381 actgaaagac attatcgcca tcctgggtat ggatgaactg tctgaagaag acaaactggt
    25441 ggtagcgcgt gctcgtaaga tccagcgctt cctgtcccag ccgttcttcg tggcagaagt
    25501 attcaccggt tctccgggta aatacgtctc cctgaaagac accatccgtg gctttaaagg
    25561 catcatggaa ggcgaatacg atcacctgcc ggagcaggcg ttctacatgg tcggttccat
    25621 cgaagaagct gtggaaaaag ccaaaaaact ttaacgcctt aatcggaggg tgatatggca
    25681 atgacttacc acctggacgt cgtcagtgca gagcaacaaa tgttctctgg tctggtcgag
    25741 aaaatccagg taacgggtag cgaaggtgaa ctggggatct atcctggcca cgcaccgctg
    25801 ctcaccgcca ttaagcctgg tatgattcgc atcgtgaaac agcacggtca cgaagagttt
    25861 atctatctgt ctggcggcat tcttgaagtg cagcctggca acgtgaccgt tctggccgac
    25921 accgcaattc gcggccagga tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct
    25981 gaagagcaca ttagcagctc tcacggcgac gtagattacg ctcaggcgtc tgcggaactg
    26041 gccaaagcga tcgcgcagct gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg
    26101 cttgaaaagc acaaaagcca gtctggaaac aggctggctt tttttacgcg tgacccgtcc
    26161 tgaagaacgt tcacatagat cctgctgata taaaaccccc tgttttcccg tttattcatt
    26221 gatcgaaata agagcaaaaa catccacctg acgcttaaat gaaggtactg ccttaatttt
    26281 ctgcagacaa aaggtgtgac gatggtcgaa aatggcgctt tcgtcagcgg ggataatccg
    26341 ttattgaaca atttatcctc tgtccatttc acgatgaaaa aaatgtagtt ttttcaaggt
    26401 gaagcggttt aaattcgttc tcaaattaca gtcaggacgc gtatgttgaa taatgctatg
    26461 agcgtagtga tccttgccgc aggcaaaggc acgcgcatgt attccgatct tccgaaagtg
    26521 ctgcataccc ttgccgggaa agcgatggtt cagcatgtca ttgatgctgc gaatgaatta
    26581 ggcgcagcgc acgttcacct ggtttacggt cacggcggcg atctgcttaa acaggcgctg
    26641 aaagatgaca acctgaactg ggtgcttcag gcagaacagc tgggtacagg tcatgcgatg
    26701 cagcaggccg cacctttctt tgccgatgat gaagacattt taatgctcta cggcgacgtg
    26761 ccgctgatct ctgtcgaaac actccagcgt ctgcgtgatg ctaaaccgca gggtggcatt
    26821 ggtctgctga cggtaaaact ggatgatccg accggttatg gacgtatcac ccgtgaaaac
    26881 ggcaaagtta ccggcattgt tgagcacaaa gacgccaccg acgagcagcg tcagattcag
    26941 gagatcaaca ccggcattct gattgccaac ggcgcagata tgaaacgctg gctggcgaag
    27001 ctgaccaaca ataacgctca gggtgaatac tacatcaccg acattattgc gctggcgtat
    27061 caggaagggc gtgaaatcgt cgccgttcat ccgcaacgtt taagcgaagt agaaggcgtg
    27121 aataaccgcc tgcaactctc ccgactggag cgcgtttacc agtctgaaca ggctgaaaaa
    27181 ctgctgttag caggcgttat gctgcgcgat ccggcgcgtt ttgatctgcg cggtacgctt
    27241 actcacgggc gcgatgttga aattgatact aacgttatca tcgagggcaa cgtgactctc
    27301 ggtcatcgcg tgaaaatcgg caccggttgc gtgattaaaa acagcgtgat tggcgatgat
    27361 tgcgaaatca gtccgtatac cgttgtggaa gatgcgaatc tggcagcggc ctgtaccatt
    27421 ggcccgtttg cccgtctgcg tcctggtgct gagttgctgg aaggtgcaca cgtcggtaac
    27481 ttcgttgaga tgaaaaaagc gcgtctgggt aaaggctcga aagccgggca tctgacttat
    27541 ctgggcgatg cggaaattgg cgataacgtt aacatcggcg cgggaaccat tacctgcaac
    27601 tacgacggtg cgaataaatt taagaccatt atcggcgacg atgtgtttgt tggttccgat
    27661 actcagctgg tggccccggt aacggtaggc aaaggcgcaa ccattgctgc gggtacaact
    27721 gtgacgcgta atgtcggcga aaatgctctg gcaatcagcc gtgtgccgca gtcgcaaaaa
    27781 gaaggctggc gtcgtccggt aaagaaaaag taattctggc tgggaaatcc ggtcatatgg
    27841 gatgaggaga taacataatc tcccgcccac aagcagtaac tataaaaata accccactct
    27901 ctacaaggct cggggcgccc gaaaaaacgg gcatacaggt tgaccgacaa cgatataaat
    27961 cggaatcaaa aactatgtgt ggaattgttg gcgcgatcgc gcaacgtgat gtagcagaaa
    28021 tccttcttga aggtttacgt cgtctggaat accgcggata tgactctgcc ggtctggccg
    28081 ttgttgatgc agaaggtcat atgacccgcc tgcgtcgcct cggtaaagta cagatgctgg
    28141 ctcaggcagc ggaagaacat cctctgcatg gcggcaccgg tattgctcat actcgctggg
    28201 cgacccacgg tgaaccttca gaagctaacg cgcatccgca tgtttctgaa cacattgtgg
    28261 tggtgcataa cggcatcatc gaaaaccatg aaccgctgcg tgaagagtta aaagcgcgtg
    28321 gctatacctt cgtttctgaa accgacaccg aagtgattgc acatttggtg aactgggagc
    28381 tgaaacaagg cggtactctg cgtgaggcag ttctgcgtgc tattccgcaa ctgcgtggtg
    28441 cgtacggtac agtgatcatg gactcccgtc acccggatac cctgctggcg gcacgttctg
    28501 gtagcccgct ggtgattggt ctgggcatgg gcgaaaactt tatcgcttct gaccagctgg
    28561 cgctgttgcc agtgactcgt cgctttatct tccttgaaga gggcgatatt gcggaaatca
    28621 ctcgccgttc ggtaaacatt ttcgataaaa ctggcgcgga agtaaaacgt caggatattg
    28681 aatccaatct gcaatatgac gcgggcgata aaggtattta ccgtcactac atgcagaaag
    28741 agatctacga acaaccgaac gcgatcaaaa acacccttac cgggcgcatc agccacggtc
    28801 aggttgattt aagcgagctg ggaccgaacg ccgacgaact gctgtcgaag gttgagcata
    28861 ttcagatcct cgcctgtggt acttcttata actccggtat ggtttcccgc tactggtttg
    28921 aatcgctggc aggtattccg tgcgacgtcg aaatcgcctc tgaattccgc tatcgcaaat
    28981 ctgccgtgcg tcgtaacagc ctgatgatca ccttgtcaca gtctggcgaa accgcggata
    29041 ccctggcggg cctgcgtctg tcgaaagagc tgggttacct tggttcgctg gcaatctgta
    29101 acgttccggg ttcttctctg gtgcgcgaat ccgatctggc gctgatgacc aacgccggta
    29161 cggaaatcgg cgtggcatcc actaaagcat tcaccactca gttaactgtg ctgttgatgc
    29221 tggtggcgaa gctgtctcgc ctgaaaggtc tggatgcctc cattgaacat gacattgtgc
    29281 atggtctgca ggcgctgccg agccgtattg agcagatgct gtctcaggac aaacgcattg
    29341 aagcgctggc agaagatttc tctgacaaac atcacgcgct gttcctgggc cgtggcgatc
    29401 agtacccaat cgcgctggaa ggtgcattga agctgaaaga gatctcttac attcacgctg
    29461 aagcctacgc tgcgggcgaa ctgaaacacg gcccgctggc gctgattgac gccgatatgc
    29521 cggttatcgt cgttgcgccg aacaacgagt tgttggaaaa actgaaatct aacattgaag
    29581 aagttcgcgc gcgtggcggt cagttgtatg tcttcgccga tcaggatgcg ggttttgtga
    29641 gcagtgacaa catgcacatc attgagatgc cgcatgtgga agaggtgatt gcaccaatct
    29701 tctacaccgt tccgctgcag ctactggctt accacgttgc gctgatcaaa ggcaccgacg
    29761 ttgaccagcc gcgtaacctg gcgaaatcgg ttacggttga gtaatatctc tcgttgatag
    29821 aaaagggctg ccgaccggcg gccctttttt ataagtgata tcaataa
//
LOCUS       DAZGAP010000039        29771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGAP010000039 DAZGAP010000000
VERSION     DAZGAP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.039"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..1294
                     /locus_tag="ACHLAM_001481"
     CDS             74..1294
                     /locus_tag="ACHLAM_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7853037.1"
                     /translation="MSIYKIPLPLNILEAAKEHITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1267..1896
                     /locus_tag="ACHLAM_001482"
     CDS             1267..1896
                     /locus_tag="ACHLAM_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO7853038.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLIPNDYNPNNVAPPEKKLLKKSIETDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1897..3057)
                     /gene="ybdL"
                     /locus_tag="ACHLAM_001483"
     CDS             complement(1897..3057)
                     /gene="ybdL"
                     /locus_tag="ACHLAM_001483"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO7853039.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3166..4254
                     /gene="hcxA"
                     /locus_tag="ACHLAM_001484"
     CDS             3166..4254
                     /gene="hcxA"
                     /locus_tag="ACHLAM_001484"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO7853040.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFENVESFKA"
     gene            complement(4264..4461)
                     /locus_tag="ACHLAM_001485"
     CDS             complement(4264..4461)
                     /locus_tag="ACHLAM_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7853041.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4574..6679)
                     /gene="cstA"
                     /locus_tag="ACHLAM_001486"
     CDS             complement(4574..6679)
                     /gene="cstA"
                     /locus_tag="ACHLAM_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO7853042.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6860..7273)
                     /gene="entH"
                     /locus_tag="ACHLAM_001487"
     CDS             complement(6860..7273)
                     /gene="entH"
                     /locus_tag="ACHLAM_001487"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO7853043.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7276..8022)
                     /gene="entA"
                     /locus_tag="ACHLAM_001488"
     CDS             complement(7276..8022)
                     /gene="entA"
                     /locus_tag="ACHLAM_001488"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO7853044.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8022..8879)
                     /gene="entB"
                     /locus_tag="ACHLAM_001489"
     CDS             complement(8022..8879)
                     /gene="entB"
                     /locus_tag="ACHLAM_001489"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO7853045.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8893..10503)
                     /gene="entE"
                     /locus_tag="ACHLAM_001490"
     CDS             complement(8893..10503)
                     /gene="entE"
                     /locus_tag="ACHLAM_001490"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO7853046.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10513..11688)
                     /gene="entC"
                     /locus_tag="ACHLAM_001491"
     CDS             complement(10513..11688)
                     /gene="entC"
                     /locus_tag="ACHLAM_001491"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO7853047.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFAGAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11877..12833
                     /gene="fepB"
                     /locus_tag="ACHLAM_001492"
     CDS             11877..12833
                     /gene="fepB"
                     /locus_tag="ACHLAM_001492"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7853048.1"
                     /translation="MRLVPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAETVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12837..14087)
                     /gene="entS"
                     /locus_tag="ACHLAM_001493"
     CDS             complement(12837..14087)
                     /gene="entS"
                     /locus_tag="ACHLAM_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO7853049.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14198..15202
                     /gene="fepD"
                     /locus_tag="ACHLAM_001494"
     CDS             14198..15202
                     /gene="fepD"
                     /locus_tag="ACHLAM_001494"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO7853050.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15199..16191
                     /gene="fepG"
                     /locus_tag="ACHLAM_001495"
     CDS             15199..16191
                     /gene="fepG"
                     /locus_tag="ACHLAM_001495"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO7853051.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16188..17003
                     /gene="fepC"
                     /locus_tag="ACHLAM_001496"
     CDS             16188..17003
                     /gene="fepC"
                     /locus_tag="ACHLAM_001496"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7853052.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(17000..18133)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHLAM_001497"
     CDS             complement(17000..18133)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHLAM_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO7853053.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGGVLLRHAMASRKQDAIMADHLV"
     gene            complement(18349..22230)
                     /gene="entF"
                     /locus_tag="ACHLAM_001498"
     CDS             complement(18349..22230)
                     /gene="entF"
                     /locus_tag="ACHLAM_001498"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO7853054.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFVRQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DSQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERALQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22227..22445)
                     /gene="ybdZ"
                     /locus_tag="ACHLAM_001499"
     CDS             complement(22227..22445)
                     /gene="ybdZ"
                     /locus_tag="ACHLAM_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO7853055.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQVSCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22448..23650)
                     /gene="fes"
                     /locus_tag="ACHLAM_001500"
     CDS             complement(22448..23650)
                     /gene="fes"
                     /locus_tag="ACHLAM_001500"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO7853056.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23893..26133
                     /gene="fepA"
                     /locus_tag="ACHLAM_001501"
     CDS             23893..26133
                     /gene="fepA"
                     /locus_tag="ACHLAM_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO7853057.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            26243..27591
                     /locus_tag="ACHLAM_001502"
     CDS             join(26243..26723,26723..27591)
                     /locus_tag="ACHLAM_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7853058.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIRSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHNDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FLGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPLEYRTEYQKAG"
     gene            27745..28365
                     /gene="entD"
                     /locus_tag="ACHLAM_001503"
     CDS             27745..28365
                     /gene="entD"
                     /locus_tag="ACHLAM_001503"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO7853059.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFEPASFHEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAVHALREFGFKYAPPVGERRQPLWPAGLYGSISHCQATALAVVSHQ
                     PTGIDIEEIFSAQAAEELKDSIMTPAEYELLVVSNLPFSVVLTLAFSAKESAFKASKL
                     QTDQGFLDYQIVNWNKQRIIICWENEIFAVQWQIRGKQLITLCQHD"
     gene            28709..29644
                     /locus_tag="ACHLAM_001504"
                     /pseudo
     CDS             28709..29644
                     /locus_tag="ACHLAM_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         7223 a         8184 c         7735 g         6629 t
ORIGIN      
        1 cttgcttcgt ttatcctata attagcggat tcatctcacg agtcaccccg taacgttcag
       61 agagcgtttt tagatgtcta tttataaaat cccgcttcca ctgaatattc tggaagctgc
      121 aaaagaacat attacctgga cacttaatac cctaccccgt atctgtgttt ctttttcggg
      181 tggtaaagat tccggattaa tgctgcatct gacagccgaa attgcccgac aaatgggcaa
      241 aaaaatctgc gttttgttta tcgactggga agcgcagttc tcatgcacta ttaactatgt
      301 tcagtcatta cgtgagtttt acgccgatgt catcgaagag ttttactggg ttgcgctccc
      361 gcttacgacg caaaattccc tttctcaata ccaacccgaa tggcagtgct gggaacctga
      421 tgtcgaatgg gtgcgtcagc ccccgcaaga tgcgataacc gatcctgact ttttctcctt
      481 ttaccagcct ggcatgacct tcgaacaatt tgtgcgtgag tttgctgaat ggttttcaca
      541 aaaacgtccg gcggcgatga tgatcggcat ccgtgcagat gagtcctaca accgttttgt
      601 cgccatcgcc agtttaaata aacaacgttt tgccgatgat atgccctgga ccaccgccgc
      661 accaggcggt catagctggt acatttaccc catctacgac tggaaagtgg ctgatatctg
      721 gacctggtat gctaaccatc aacagctctg taacccactg tataatataa tgtatcaggc
      781 aggcgttcct ctgcgtcata tgcgaatttg cgaacctttt ggcccggagc aacggcaagg
      841 attatggctc tatcacgtta tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag
      901 cggggtaaaa agtggcggca tttacgccgg acatgataat catttttatg gtcatcggaa
      961 aatcctcaag ccagaacatt tagactggca agaatatgca ttattgctgc tcaatagcat
     1021 gccggaaaaa acagctgagc attaccgcaa taaaatcgcc atttatttgc actggtatca
     1081 gaaaaaaggc atcgaagttc cacaaaccca gcaaggggac attggcgcga aagatatccc
     1141 ctcctggcgg cgaatatgca aagttttact caataacgat tactggtgcc gggcattatc
     1201 atttagtcct acgaaagcga agaactatca gcgttataac gaacggataa aaggaaaacg
     1261 tcaggaatgg gggatactat gcaacaacga ttaacgcagg atttaaccca atttctcgcc
     1321 agcttgccgg aagatgatcg catcaaagcg attaatgaaa tccgcatggc gatccatcag
     1381 gttagcccct ttcgtgaaga gccagtggat tgcgttctgt gggttaaaaa cagccaactt
     1441 atacctaatg attacaaccc aaacaacgtg gcaccacccg aaaaaaaact gctgaaaaaa
     1501 tccattgaaa cagatggttt tacccaaccc attgtggtca cgcatacgga taaaaatgcc
     1561 ctggaaatcg tggatggttt tcaccgccat gaaattggta aaggctcatc atcgttaaaa
     1621 ttacgcttaa aaggttattt gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc
     1681 gccgcgacta ttcgtcataa ccgcgctcgt ggtcgccacc aaatcactgc catgtccgaa
     1741 attgttcgtg aactcagcca gttgggatgg gacgataata aaattggcaa agagctggga
     1801 atggacagcg acgaagtgct gcgcctgaaa caaattaacg gcctgcaaga gttgtttgcc
     1861 gaccgtcaat attcccgtgc ctggactgtc aaataactac agctggcgca ggcgttcagc
     1921 tgctgccagc aacgtcgatt ccttcttggc aaaacagaga cgaatcagtt tatgggggaa
     1981 gggatcggcg caaaataccg acagcgggat cgccgctaca ccgtgctccc gcgtcagcca
     2041 ctggcaaaac ttaacgtcat ctagcgaaga aaccgcgctg taatccacca gcaaaaagta
     2101 tgtaccttcg cacggtaaaa tctccagccg actctcactt aaggcattca ccagaatatc
     2161 gcgtttctgg cgataaaagt ccggtaacgc aagataatgc tcaggttctg cacgtagcat
     2221 atcggcaagc gccaactgtg ccggggtatt caccgaaaag gtcagatact gatgcacttt
     2281 gcggatctcg gcgctgatgg gcgcaggcgc aacgcaataa cccactttcc agccggtcat
     2341 atgataggtc ttgccaaacg atgacaccgc caccgctcgc tcacgcaatt gcggatgcgc
     2401 cagcacgctg gcatggccct gttgtgaaaa attgatgtgc tcgtagactt catcgctaat
     2461 gacaaaaatc tcgtgcccgg cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca
     2521 gacagttgca ctggggttat gcggggtgtt gaggatcacc agtcgggtgc gctcgcttaa
     2581 caatgcggca aattcctgcc agtccacgcg aaaatgcggt ggttgcagtg caatacgttt
     2641 cactattccc cccgaaagcg cgatggcggg cgcgtaactg tcatagctgg gatcaaagca
     2701 gatcacttca tcaccattgc gcaccaatgc ggtaatcgct gcatataacg cttccgtcgc
     2761 ccctgccgtt acggtgatat cgctatcggc atccggttga tagccataca gacgttccgt
     2821 tttctgagca atcgcctcgc gcaaggcctg cacgccggtc atgggcgcgt attggtttgc
     2881 cccctgggca acgtggtacg ccagccgctc ctgtaaatag cgcggaccat caaaatcagg
     2941 aaagccttgt gaaaggttaa tcgcctggtg ttgctgcgcc agcgcgctca tccgggtgaa
     3001 aatagtggtg ccaagttgtg gaagtttgct ttgtggaatc agagggttat ttgtcatttt
     3061 tattgtacct gacgaagttg cgtgttgaag gcactataac acgatgctag tatttggcaa
     3121 tcaagacgtt tagatgtcta aataaaacaa taaggacaac acaacatgcc tcacaatcct
     3181 atccgcgtgg tcgtcggccc ggctaactac ttttcacatc ccggaagttt caatcacctg
     3241 cacgattttt tcactgatga acaactttct cgcgcggtgt ggatctacgg cgaacgcgcc
     3301 attgctgcgg cacaaaccaa actgccgcct gcgtttgaac tgccaggggt aaagcatatt
     3361 ttgtttcgtg gtcattgcag cgaaagcgat gtgcaacaac tggcggcgga gtccggtgac
     3421 gatcgcagcg tggtgattgg cgtcggcggc ggcgcactac tcgacaccgc gaaagccctc
     3481 gcccgccgtc tcggtctgcc gtttgttgcc gttccgacga tcgccgccac ctgcgccgcc
     3541 tggacaccgc tctccgtctg gtataacgat gccggacagg cgctgcatta tgagattttc
     3601 gacgacgcca attttatggt gctggtggag ccagagatta tcctcaatgc gccgcaagaa
     3661 tatctgctgg cggggatcgg tgacacgctg gcgaaatggt atgaagcggt ggtgctggct
     3721 ccgcaaccag aaacgttgcc gctaaccgtg cggctgggga tcaataatgc gcaggccatt
     3781 cgcgacgtct tgttaaacag tagcgaacag gcgctgagcg atcagcaaaa acaacagtta
     3841 acgcaatcat tttgcgatgt ggtggatgct attattgctg gtggtgggat ggtcggtggt
     3901 ctgggcgatc gttttacgcg tgtggcggcg gcccatgccg tgcataacgg tctgaccgtt
     3961 ttgccgcaaa ccgagaagtt tctccacggc accaaagtcg cctacggaat tctggtgcaa
     4021 agcgccttgc tgggtcagga tgatgtgctg gcgcaattaa cagaggcgta tcagcgtttt
     4081 catctgccga ctacgctggc ggagctggaa gtggatatca ataatcaggt ggagatcgac
     4141 aaaatgattg cccacaccct acgtccggtg gaatccattc attacctgcc agtcacgctg
     4201 acaccagata cgttgcgtgc agcgttcgaa aacgtggaat cgtttaaagc ctgacgtaca
     4261 aatttagcag cagcgcgcgc cgccttttcc accgtagcgc gcgtcctgcc gctcccggaa
     4321 aaactcctcg taggtcatcg gtgtttgatc gggatggtta acccgcatat gttcgacata
     4381 gttgtcgtaa tcaggcatac caatcatcag tttcgccgcc tgacctaaat attttccggc
     4441 ttttgccagt gaatcaaaca tctaaacctc cggtaataca aatgccctcc tgaattaaga
     4501 gggcttaatt gctatcaaca cgctcgggat gagagtaagg ggaacgctca gcacattccc
     4561 cctcactctg actttagtgc gcgcctttcg cctgcgccac gatctcctcg acattttccg
     4621 gcatcggctc atatggcgtt tctttcgccg ttggcttcgg ctctttcaat gccaccagtg
     4681 ccgtcttaat cgagaacagc gccagaacca ccacaaccac cataaagaag atggttaatc
     4741 cggcatccag acggttgttg aacaccagtt gcgccagttg tgactcagta tactgcgacg
     4801 gaatattgcc gctgtcgatc attgcctgga acttgttagc aatcgccagg aagccgactt
     4861 tcgcatccgg gctaaacgct ttctgccagc ctgcggtcag ggtacaaatc agcagccagg
     4921 ccgttggtac cagcgccacc caggcgtaac gttgacgttt cattttgaac agcaccacag
     4981 cacagagcat cagcgccatc cctgccagca tctggttggc aataccaaac agcggccaca
     5041 aggtgttaat accgcccagc ggatcgacca cgccctgatg caggaagtag ccccacgcca
     5101 gcacgcacag cgccgttgcc agcaggttag caggcagtga atcggtccgt ttcaggcccg
     5161 gagacaccac gcccagcaga tcctgcaaca taaagcgcgc agcacgcgta cccgcatcca
     5221 ccgccgtcag aataaacagt gcttcaaaca gaatggcgaa gtgataccag aacgccacat
     5281 ccatcatgcc gcccagcgca ccgtgcagaa tgtaggccat ccccaccgcc agggtcggcg
     5341 caccgcccgc acgagaaatg atcgactgtt cacccacttc gctggcaatc tggtttagcg
     5401 tatccggggt aatcgcaaag ccccagctac tcaccacctg cgcggcagaa gcaaccacat
     5461 ccgccgtccc tgccggagcc agcaccgcca tcgggctgtt catggcgaag tacacacccg
     5521 gatcgatgat acaggcggaa accagcgcca taatcgccac gaaggattcc atcaacatcc
     5581 caccgtagcc gataaagcac gcctgccctt cgttcgccag catcttcggt gtagtgccgg
     5641 aagagatcag tgcatggaag ccagacaccg cgccacaggc gatggtgata aacaggaacg
     5701 ggaacaggtt accggtccat accgggccag tgccatcgac aaatttagtt agcgcaggca
     5761 tggtcagcgt cgggcgcata atcaaaatgc ccaccgccag accgacgata gtcccgattt
     5821 tcaggaaggt tgagaggtag tcacgcggag ccagcagcaa ccacaccggc agcaccgccg
     5881 ccacaaaacc gtaacccacc agcatccagg tcagctgcac gccggtaaag tcaaagtacg
     5941 gtgcccacgt cgggctttct gccacccagc cgccagagat aatggcgaaa ataaggaaca
     6001 ccagaccaat caccgacact tcgccaatac gccccggacg caaatagcgc aggtagatcc
     6061 ccataaacag cgccagcgga atggtgaacg caacggtata cgttccccac gggctatgag
     6121 tcagggcttt caccacgatc atcgccagca cggcaaggat aatgaccatg atcataaagc
     6181 aggccaccag cgcaatcacc ccggcggttg gccccatctc ttctttgacc aactcaccca
     6241 gcgagcgacc atcgcggcgc gtagaaacaa acagcaccat gaaatcctgc accgcaccgg
     6301 cgagaaccac cccagcgagc agccagatca tccctggcag gtaacccatt tgcgccgcca
     6361 gtaccggccc aaccaacgga cctgctccgg caatggccgc aaaatggtga ccgaacagca
     6421 cttttttgtc cgtcggcaca tagtccagcc cgtcgttatg gcgcaccgct ggcgtcatac
     6481 gcgtcgggtc aaccgccagc acatttttgg cgatatacag cccataaaaa cggtaagcga
     6541 tcagatagat acagaccgac gccaccacaa tccacagcgc gttgatctgt tccccacgat
     6601 ttaaagcaat gtatcccaga gcaaatgctc ccattacaga gagcactgtc cagacgaggt
     6661 atttccctga tttgttcata gttgttatcc gtgtgcgtgt ccatagagat gttacatttt
     6721 gtttctataa catctttgta caatttaaca acacggcaac catgtaaatc ggagttgaaa
     6781 ccaatattta acttaacaat gttaactcga tcactccgtt aaccgagttt tcctgcaatc
     6841 tcatttaatt ctgtcgggct catcccaaaa ctgccgtacc cagccgacaa gtgcagcaac
     6901 gccgcccctg ttcatcgaaa acaacaattt cccagctctg attttgccga ccaagatgca
     6961 gcggctggca gacgccgcgt acctttccct cagacaccgg gcgatgatgt gtggcgttaa
     7021 gttccgtgcc caccacacac tgtccgtcgc gagtcatcat aaatccggcc atcgatccca
     7081 gcgtttccgc cagcgccgcc gacgcgccgc catgtagtaa accgaacggc tgatgagtac
     7141 gggtatcaac cggcatttcg gcttccagca catcatcgcc caggcgggta tacacaattc
     7201 ccagatgcgc caccattgtg ttatcgctgg tggcgttcag ctcgtcgagc gttaaatggc
     7261 gtttccagat catgcttatg cccccagcgt agagccgcca tctaccacaa tatcctgtag
     7321 ggtgatatgg ctggcgaggt cagaggcgag gaacaaaatc gtgttggcga tctcttgtgg
     7381 acgggcgatt ttccccagcg gaatgccaag tttaaactgc tcgccaaagc cgcgaatacg
     7441 ctgctcttcg gcgtcatcgc tcacccacag cgtgcgttgc atatcggtgt cggtggagcc
     7501 tggcgaaacc acattacagc gcacgccgct acccgccagt tccagcccaa cgctcaacgc
     7561 caggcttttc agcgccgctt tcgacgcgcc ataagcactc ataccaatgc gcggcgtgtg
     7621 tgcggcgtcg gacgcaacag taacaatcgc cccgccccgc tgacggcgaa actggttcat
     7681 ggtctgctgg aacaggttaa acgcaccgcc gacattaacc gcaaaagtct gctgccagtc
     7741 ctccttactg agctgatcgg tcgcgcccat gcgcaaaatt cccgccgcat tgaccagcac
     7801 gtccagtcgc tccgtttcag ctaacagtcg ctgacatacc tgcgccacct gcgcagcgtc
     7861 ggcaacatcc atcacttcgg tcgcaaaggg atattgctcc tgagtgaacg cttgatcaaa
     7921 acctgtaact ttcgctcccg cctcaacaaa cgccagcgcc gtggcgtagc cgataccttt
     7981 ccccgcgcca gttacccaga catttttacc gctgaaatcc attatttcac ctcgcgggag
     8041 agtagcttcc accaggcgtc gatggtcggg tttttcgcca gcatgacaaa gtcgatatcg
     8101 ccatgcactt tgcgccagcg cgccgccagc gccatcatcc gcaccgaatc cagaccgtag
     8161 tcgatcaggt tgtcgtcatc gaacggttca tcggactcgt ccagcaacgg caggatcacc
     8221 tcacgcagcg ccgctttgct ggcggggata ggtgctggca gtaattcttc ggtcatcacc
     8281 actcggccag aacgtccggc cacatatttc agcgacatca aatgctcgtc acggctgaaa
     8341 tcggccagcg catctgccac cataaacggt ttaatatcgc gcataaatgc gtcggttgcg
     8401 gtggtcatac agccaatgtg ggcatatacc ccggtaataa tcagctggtt acgtccactc
     8461 tctttcagca tttgctccag cggggaacga tgaaacgcgc tgtagcgcca cttcaccagc
     8521 accgtgtcgt cggcatctgg cgtcaggcta tccaccacct tttgctgttc cggcgaacgg
     8581 gtcaggccag gcccccacat atcattcaac agcgcccgat cttcatcgct ctgctctttc
     8641 ggctgggcgg tgtaataaac cgggatattg tgctgtttgc agtagtcgcg tagtgccgca
     8701 atattcgcga tcacctgctc catcatcggg cagttctcgc cccagaagct gacaaaatag
     8761 tcctgcatat catggattaa caacgcggca cgttgcggtt caaaggccca gtcgacttta
     8821 ttctgcggaa tatcgtgaga ctccggcagt gcgtaagcct gtaattttgg aatagccatc
     8881 gtgttctctc cttcaggctg atgcgcgtga cgccagccac tgacgtaatt gttttttatc
     8941 gactttcccg accgccgtca gcggaagtga atctacacac tccacgcgat ccggtaattt
     9001 aaattcggca ataccctgtt cacgcaggaa acgacgcacc tgcaccgcgc gcagcggctc
     9061 ttttaccacc agataagcac agcttttttc gcccatcagc tcatcttcca tgctcaccag
     9121 tgcggcgtag atcaccgccg gatggcgcag cagcaggttt tcgatctctt cggcagcgat
     9181 cttctcgccg ccacggttga tctgatcttt ctcgcgcccc tgcacagtga tgtaaccctc
     9241 tggatcaata gagatcagat cgccggaaca gtaaaaaccg ttggcatcaa aggcgctggc
     9301 attgtgctgc gggcttttgt aatagccacg gaaggtatac ggaccgcggg tcatcaggcg
     9361 tccaacttcc ccttgcggca gtggatttcc ttcggcatcg gcaacccaca cttcatcgtc
     9421 cggacacatt gggtaaccct gggtatggat aattttctcc gcgctatcat caagtcgggt
     9481 gtagttcact agtccttccg ccatgccgaa tacctgctgt aactggcagc caatctctgc
     9541 gggaatacgc gccgcaagcg tggcagaaag acgtgcacca ccgacctgta acagtttcag
     9601 cgaggcaagc tgcgcccggc tttcgccttc agccagtgcc tgcaaccaca ggctgactgc
     9661 gggcggcacc agcgcggtaa cgttgacctg atgtttttca atcaatggga agcaaagcgt
     9721 ggcgctgggg tcggcagcca gaacgacagt gccgccagcg agaaataccc ccaacgatcc
     9781 cggcgaactc atggcgtagt tatgagccgc cgggatcgcg cacaggtagc gcgtctgttg
     9841 tgtgaactga caaatctcga cgctacgacg cacgctgtag tagtagtcgt tatgagtgcg
     9901 cggtatcagt ttcggagtgc cggtggtgcc gccggaaagc tggaaatagg ccacttcatc
     9961 agcaggagat ggcgtggcag taaaatcctc agccggatgg ttaatcgcat cctgcaagtt
    10021 atgctcaccg ctgtcgttga gcagctgcac cacgcgaatg gaagaatgct ctgcaacaaa
    10081 tgtgttgagg aaatcatccc cgctaaatag cgcatgttga cgatcggcaa tcagtaacgc
    10141 cggttcaatc tggctggcat aggcgttcag ttcactacgc tgatggctga acagcgccag
    10201 caccggcgca acgcccagct tcagcagcgc gaaaaaggta atatacaatt cagcgacgtt
    10261 acccagttgt accagcgcgg tttcaccagg tttaatgccc tgacggcgca aactacaggc
    10321 gaggttatcc gccgcctgat tcagctcccg gtaactcaac tgccgctcgc cgtcgataac
    10381 cgcgatgctg tcactcgcag cgtggcgagt cagaatgtcg gtcagtggca aatcctgcca
    10441 gtagcctttt tcccgatagc gacgggcaaa ctcttccggc cagcgggtga atggaatgct
    10501 catcctcgct ccttaatgca atccaaaaac gttcaacatg gtagaaagtt tgacgcctgt
    10561 ttcgcgccac tcacccaacg gtgaggacgc aggcacaatc cccgctccgg caaacagacg
    10621 cacctgattt tcccgcagct tcgcgcagcg gatggtcacc acccattcac cgttaccttc
    10681 gctgtcacac caacccacaa tgccgccaaa cagttcgcgg tcgaacggct ccagttcagc
    10741 aataacctga gtcgcggcct gatgcgggaa accgctcagc gcgggggtcg gatgcagcag
    10801 acaggccaga gtcagtgcgt tttcttgcga attcgcttta ccttcaaagg gagttgcgag
    10861 atgccacagc gtcggcgtgg taatcaattg tggagaggag ggaacgtgta actcactact
    10921 gcgtttgcgc agtacctctt tcatcgcctg agtcaccagt tcatgttcat ggcgatcttt
    10981 ttctgacgcc agcagacgat tacccgcttc gcgatcgagc acttcatccg gctgacgacg
    11041 cgcggaaccg gctaacggaa tggagctaaa acgctcgccg tctttacgta gcagcagttc
    11101 cgggctggcc cccagcagga cgccgccatc agccagcggg acatggaagt tgtaactaac
    11161 cgggttttgc gcaatcaacc gttccagcaa tacgccacta tcaatggcgg cgtcagtggt
    11221 gatatcaatc aaccgtgaca acaccacttt gtcgacctgc ggcgtggcgg taagtgcggc
    11281 ggcgcgggca accatctgtt caaacgtggt ttgttccgga attgcctggc gttccaccac
    11341 attcagcgac tggctgcggg tgaaacggcg tgcggaagct tgtttttcct gacgggagaa
    11401 cgactgccag gattcgggaa tatacagcga tgaaggctga cgtggatcga agggaatcgc
    11461 cccgaccatc accggatttt tgatgccctg cgctttggca ccggcaaaca gcgcggcgag
    11521 tttttgctgg aagggactgt cgggcgaatc cccgttcaca gccggttcat cgaagcgggc
    11581 gaaacatcct gacgtcgtaa aactgcggta cggcgacata aagaaaaagc gattgggcgc
    11641 aagtgttgcc atggtctgct gtacttcctc agccagtgac gtatccatat catcctccac
    11701 aaaatgataa aggctttaat aatgattatc atttatattt tcggtcgcta acctaaaagc
    11761 aaacgcgcac tatgtcaact cttgaggtaa cgcaatctgc aactcggggt tgcatctggt
    11821 tgtggttaca atgaaaatga gaagcattaa cttcattaat aacaggaagc tgatttgtga
    11881 gactcgtccc gctctaccgc aacgcccttc tattaacagg acttttgctt tcaggaatag
    11941 ccgtagttca ggccgccgac tggccgcgtc agattactga cagccggggc actcataccc
    12001 tggaaagcca gccgcagcgt attgtttcca ccagcgtcac cctgactggc tcactgctgg
    12061 cgattgatgc tccggtgatc gccagcggcg cgaccacgcc gaataaccgc gtcgcggatg
    12121 accagggctt tttacgccag tggagcaagg tggcgaaaga acgcaaactg caacggctct
    12181 atatcggcga gccgagtgca gaaaccgttg ccgcgcaaat gccggatctg attttaatta
    12241 gcgcaaccgg cggtgattca gcgctggcgc tgtacgatca gctttccacc atcgccccaa
    12301 cattaatcat caattacgac gacaaaagct ggcagtcgct gttaacgcag cttggcgaaa
    12361 ttaccgggca tgagaaacaa gcggcagagc ggattgcaca gtttgataag caactggcgg
    12421 cggcgaaaga gcaaatcaaa ttaccgccgc agccggtcac tgccattgtc tataccgccg
    12481 ctgcacacag tgccaatctc tggacgccgg aatcagcaca agggcagatg ctggaacaac
    12541 tcggctttac gttggcgaaa ttgcccgcag ggttaaacgc cagccaaagc cagggcaaac
    12601 gccatgacat cattcagctt ggtggggaaa atctggcttc agggttaaat ggcgagtcac
    12661 tattcctgtt cgccggtgat cagaaagatg ccgatgcgat ttatgctaat ccgctgctcg
    12721 cgcacctgcc tgcagtacaa aacaagcagg tttatgcgct gggaaccgag acgttccgtc
    12781 tggattacta cagcgccatg caagtgctgg aacggcttaa ggcgctgttt taagcattaa
    12841 ctgtcggacg ctgtcacctg cggcggcgtc tggcgaaaac gccgcaactc caccagcacc
    12901 agcaataaca acacgccgat aatcaacaaa ccaaaaccgc tcgcgcttgc ggaagcaacc
    12961 ggtgtcatca tcgcgcccaa accgcccagc agcgccgcgc ctatggcatc gcccgtcacg
    13021 ttttgcgccg tccacaaacc gttaatccgc cctaacatcg cttccggggt ttgcgtttgc
    13081 agcattgtgt attgcagcaa cgaactaacc gcgctcagcc agccgaacag cgccagacaa
    13141 accacgccta aaatccacat cggcatcagg ccaaacagac caatggcgag gaacgatccc
    13201 agcgtggaaa gcagcatcaa taaccctggt cgcgcgctat gtgccagctt cccgctggtt
    13261 aacgcgccaa tagccgcacc gagcgggatc gccgcataga gaaaaccaat ctgtgccgcc
    13321 gacatctgcc agttgtcagc cagcgccgga tacaggaccc gcaccgcgct cgccatcgtc
    13381 aataaaccac ccaacagcgc aatccctcct accagcgggc tggcgagcag aaaacgaaat
    13441 cctgccagta atgatttcaa cggatgctca cgcggctgcg gtggtggtgg taacgccgga
    13501 aggcttaaca acggtagcaa ggtaataaac gtgcccgccg ccgccagccc gtagttccag
    13561 gccacaccgc cagtcgccag cagtaaaccg ccaatcatgg gcgaaatcac cgatcccaga
    13621 cgcacggtca acatggtgat cgccccggcc tgcattaagt tttcacgccc taccagtgct
    13681 ggtgtcgccg ccagtagcgc cgtaacgcca agtgatgcga aaaaaccatc ccataaacca
    13741 agtaaataga ttgccagcaa tgacggctcc ggcagcagtg cattaaggca cagtccaatg
    13801 aagccaatgc cacaggtgcc gcgcgccagc aaaatcactt ttttgcgctc atagcgatcc
    13861 gccagcacac cgccgaccat caggccaaca aacatcgcgc cgccggtcag cgtcaccgaa
    13921 agccccacct gccaggtaga atgcgtcatt atctggatct gcaccggtac cgcgacgccg
    13981 agcaaaccca gagacacaat tgagataaaa cgagcgagga atactgcgcg aaacgccggg
    14041 tgcgttttca acaggctgag gttaagcagc caggattgtt tattcattac aatgccttgc
    14101 catcaaatat tttaaatacc tattcttagg ctgcacatgc taacatatcc aaataagatc
    14161 gataacgata attaatttca ttatcatgga agttcgtatg tctggttctg ttgccgtgac
    14221 acgcgccatt gccgtgcccg gattgctgtt attactgatt atcgcgacgg cattaagcct
    14281 gctcattggg gcaaaatcac tccccgcttc cgtagtgctg gaggccctct ccggcacctg
    14341 ccagagcgcc gactgcacca tcgtgctcga cgcgcggctg ccgcgcaccc ttgccggttt
    14401 actggcaggc ggcgcgcttg ggcttgccgg ggcgttaatg caaaccctca cccgaaaccc
    14461 acttgccgac cccggcttgc ttggcgtaaa cgccggagcc agctttgcca ttgtgctggg
    14521 tgcggcgctg tttggttact catccgcgca ggaacaactg gcgatggcct tcgccggggc
    14581 gctggtggcc tcgttgattg ttgcctttac cggcagccag ggcggcgggc agttaagtcc
    14641 ggtgcgttta accctggcgg gcgtggcgct ggcggcggtg ctggaagggc tgaccagcgg
    14701 catcgccctg cttaatcctg acgtctacga tcaactgcgt ttctggcaag ccggttcgct
    14761 ggatattcgc aatctatata ccttaaaagt tgtgctgatc ccggtgctga tcgccggagc
    14821 aaccgcgcta ttactgagcc gcgcgctgaa cagtttaagc ctcggcagtg acaccgcgac
    14881 agcgctgggc agccgcgtgg cgcgcacaca gttgattggt ctgctggcga ttaccgtgct
    14941 ttgtggtagt gcgacggcgg tagttggccc gattgccttt attggcctga tgatgccgca
    15001 tatggcgcgt tggttggttg gagcagatca tcgctggtcg ctgcccgtca cgctacttgc
    15061 cacccctgcc ctgctgctgt ttgccgatat catcgggcgg gtgattgtgc ccggcgaact
    15121 gcgagtttcc gtggtcagtg cgtttattgg tgcaccggtg ctgatcttcc tcgtgcgacg
    15181 taaaacgcga ggtggcgcat gatttacgtc tctcgccgat taattatcac ctgtttgctg
    15241 ctggtttccg cctgtgtggt tgcaggtatc tggggattac gcagcggtgc catcacgctg
    15301 gaaacctcgc aggtattcgc cgcgctgatg ggcgatgcgc cgcgcagtat gacgatggtg
    15361 gtcaccgaat ggcgtttacc acgcgtgctg atggcgctgt tgattggcgc ggcgctgggc
    15421 gtcagcggcg cgatttttca gtcgctgatg cgtaacccgc tcggcagccc tgacgtaatg
    15481 ggctttaaca ccggggcatg gagcggcgta ctggtggcga tggtgctgtt tggtcaggat
    15541 ctgacggcta tcgcgctggc agcaatggcg gggggtattg tcacctcgct gttggtctgg
    15601 ctgctcgcct ggcgtaacgg tatcgacacc tttcggttga ttattatcgg tatcggtgtt
    15661 cgcgccatgc tggtggcctt taatacctgg ctgttgctga aagcgtcttt agaaacggcg
    15721 ctaacagcgg gtttgtggaa tgccggatcg ctcaacggcc tgacgtgggc aaaaacctca
    15781 ccttccgcgc caatcattat attgatgctc attgccgcct ccttgctggt gcggcgaatg
    15841 cgcttgctgg aaatgggcga tgataccgca tgtgcgctgg gcgtcagcgt cgaacgttcg
    15901 cggctgttaa tgatgctggt cgccgtagtg cttaccgctg cggcaacagc gcttgccggg
    15961 ccgatttcct ttattgcttt agtcgcgccg cacattgccc gccgcattag tggcaccgct
    16021 cgctgggggc taacccaggc ggcgctgtgc ggggcgttgt tactgctggt ggccgatctc
    16081 tgcgcccaac tactgtttat gccgtatcaa cttccggttg gcgtcgttac cgtcagcctc
    16141 ggtggtattt accttatcgt cttgttaatt caggagtctc gcaaaaaatg accgaatcag
    16201 tagcccgttt gcgcggcgaa cagttaaccc tcggatatgg caaatatacc gttgcggaaa
    16261 atctgactgt agaaatacct gatggtcact tcacggcaat tatcgggcca aatggctgcg
    16321 gtaaatccac gttgctgcgt accttaagcc gcctgatgac gcctgctcac gggcatgtct
    16381 ggctggatgg cgagcacatt caacattacg ccagtaaaga ggttgcacgc cggattggcc
    16441 tgttggcgca aaacgccacc acgccgggag atatcaccgt gcaggagctg gtggcgcgtg
    16501 ggcgttaccc gcatcaaccg ctgtttaccc gctggcgcaa agaagatgaa gacgcggtaa
    16561 cgaaagcgat gcaggccacg ggaatcactc atctggcaga tcaaagcgtg gatacccttt
    16621 ctggcggaca acgccagcga gcgtggatag caatggtgct ggcccaggaa acggcaatta
    16681 tgctgctcga tgaacccacg acctggctgg atatcagtca tcagattgat ttgctggaac
    16741 tgttaagcga actgaaccgc gagaaaggct ataccctggc ggcggtgctg cacgatctta
    16801 atcaggcctg tcgttacgcc agccatttga ttgcattgcg ggaagggaaa attgttgctc
    16861 agggagcgcc gaaggagatt gtcactgctg aactgatcga gcgcatttat ggtctgcgct
    16921 gcatgattat tgacgatccg gttgccggaa cgccgctcgt ggtgccgctc ggacgaacga
    16981 caccttcaac cgcgaagatt taaactaagt ggtctgccat tatggcatcc tgttttctgg
    17041 atgccatcgc atggcgcaat agcacgccac cacaagccac tataccgccg atcaacgcag
    17101 aaaggatcac aataatcgcc ttacccggac cgtctttttt tacgggtaat gacggacgca
    17161 actgatattt aaacggtgta aaattcacat cgttgacatt tgtttttgtt aattgctcga
    17221 ctagatactg acgattacgt aattcaccgt tcaattccgc aacgtcagtg actgcttttt
    17281 caatttccag tttgcgttca ataccgtctg cgccgagaga aatagagaaa tcggggtcat
    17341 ctttaacggc ctgaccatta ctgtatacgg gctttttaat tcctgccgcg ttggcaatgt
    17401 cgagtgaata attgaggcgc tgaatgtttg catcaagttg atttttcgtt ttaatgcgat
    17461 cctgagccag tttttctttt tcaaactggg ttttgatctc cagtttatta cggacgtttt
    17521 ctatcgactc tttcaccacc aacgcagaga tataatcgat atacccggcc aacactttct
    17581 gtgcctcttc actggtgggt gcggtgaaac ttagcgtcca ggaagtatac aatgcagact
    17641 catctttttt cttactggca ttgtcatcaa ccgctttcat tttttcgctc aatgcgacaa
    17701 ttgcgcgatg caaatccagt tcgtcgattt tcgcctcttt taattggtcc atcacataag
    17761 gtgttgagcg caggtactct tccagcaagc taaccgactg aaacttcttg ataaacaggt
    17821 taaatgcttc tgtacgatca attttgatat ccagatccag cacacgaagc ttagtgaatg
    17881 ttttctcaag cgcctgccac tgaacaggtt ctggaggcgt gacaaccgcc gcgctggtcc
    17941 atttttgcgg caggatgaaa gagatcagta aacctgcgca ggcaaacgca aaaacgaccg
    18001 ccatgaccgt ttttttggcc cgccataaaa cttcaattag attaagtaaa tcaacttcat
    18061 tattactggg cgacgccaga ggatactcgg gaaaatgagc ttcacttccc tgtttaatat
    18121 tcagtgatga catggggtat aacctgaaaa tgagtccaat aagagtaaat ctccacggcc
    18181 cccactctac cagttggtga gatttatccg aagctgaagt ttgtaaatta ctcccactga
    18241 atgcaacatt cactctattt cagagaaata cccaccaaat ttataaatta aataaatgat
    18301 aaacccataa ttatttcctg taattatggg tttatagata atattaattt acctgtttag
    18361 cgtagcgcga ataatcggcc caattttttc aaacgcccct ggagagataa tatccacatg
    18421 cgcacaatcc tgacgataga tatccagctc ggctatccac ggcgaccagg cgcgttcggg
    18481 attcatacct tcctgaagcg cgcgttcagc aacaaacagc gtcgctttac cgtcaaacgg
    18541 tacactatga gcagtcgtca gcaggcgcac agcatcagcg tagttgcctt caatggtggt
    18601 aaacaactcc gttgaagtac ttccctgctg tgccgccagg aaggcctcgc gctcgcggtt
    18661 aatctccgcc agcacttccg ggtccagacc attggcttct ttttcctgcc agttttgcgt
    18721 ttctggcggc caggtatcca gcaagccaag aaatgccacc tgttcgccac gggcacgcag
    18781 tcgcgccgca atgccctgcg ccagcgtacc gccaagtgaa taacccagca ggtaataagg
    18841 accgtgcggt tgttgttcaa gtaacgttgc cagatgcgct tcgcagactt catccaggtt
    18901 tgccgccgtc tgcatggggc catgagggcg cggcgactga atgccgataa tcgaccattg
    18961 tgaatcgaga taacgcgaga gcacgctaaa ctgccaggca aaaccggagg ccggatggaa
    19021 acaaaacagc gtcgggccat taccttcacg caacggcaga atggtttcga atcccatgcg
    19081 ctgggtgctg tcttcttcac catcaataat cgttgccagt ttggcgacag ttgacgcgac
    19141 catcacctgc cccggcgtca cctgacgaac aaactgtcga cttaactgcg ctgccagttt
    19201 cattgccagt agcgaatgac cgccaagcgc aaagaaatca gcatcggcat cctgcacgtc
    19261 acaacccagc aacgacgaga atgccgcggc gataatcgtt tcactgcccg ctttcggcgc
    19321 acgccccggc gcttgtgctt tcagttccgg caacggtaag gctttgcgat ccagcttgcc
    19381 gttggcgcta agaggtaact gtggaagttg cagcagaacc acgggcacca tatgtggtgg
    19441 caatgtttcg cgaagctgag cctgtaatgc gctggtatcc aacggcaggc ccgattgcga
    19501 caccagatag cccaccaact gacgcgcatc accaccagtg gctgccgcct ggttaatcac
    19561 acaggcgtgg gtaacggctt gttcgacatc cggcagcgcc tgcatcacgc gatcgatttc
    19621 gcccagttcg atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc cgaggtactc
    19681 caccgcgccg ttatccagcc agcgggcaac gtctccggta cggtacatcc gttcgccagg
    19741 ggcaaaagga tcggcaataa agcggctggc ggtcagatcg ggtcgtccaa gatacccctg
    19801 cgccagttga ataccggtga gatagagatc tcccgccaca cccggcggca ccggatgcat
    19861 catcgcatca agaatacgca ggcccgtatt ccacaccgga taaccaatcg gcacactgct
    19921 gccgcgcacc tgtgccagtt cctcgccaaa agccggatac cagctgacat ctaccgccgc
    19981 ttccgtcggg ccatatagat tatgcaacgg cacgctggtt aactgttgcc attcgcggca
    20041 taaatcagcg ggtaaggcct caccactaca gaaaacctgt ttcaacgtcg cgcaattctg
    20101 gcgagcggtt tgcggagtca gcgatgcaac aaatgccgcc agcatcgacg gcacaaagtg
    20161 cgtggtcgtt acaccatatt cggcaaagaa ttgctgcata gcgagcgggt cgcggtgcgc
    20221 ttccggttca gccatcacca gtttagcccc ggcaataaac ggccagaaaa actcccacac
    20281 cgagacatca aaactgcacg gcgttttttg ggcaacgaca tcttcgcctg taagcgggta
    20341 gtggttttgc atccaaagca gacggttaac gatagccgtc tgcccgacca ttaccccttt
    20401 cggcctgccg gtggagccag aggtaaagat gatataagcc gtgtggtgcg gttgtgaaag
    20461 tttcagcggc gcactgccct gcggtgtaag cggggcgtta tagcaaaggc tggttaaatc
    20521 tggaacatcg gcaaagcgcg gcagttgatc gtcggtggtg attaacagcg acggacgcgc
    20581 atcttccagc atcattttca ggcgatcgtc cggatagccg gtatccagcg gtaaccaggc
    20641 cgcaccagcc tcaacaattg catgtagcgc cagagtcaaa aagaccgagc gcggtagcgc
    20701 caccgccacg ctgtcgcctg gtttaacgcc gcgctcacgc agcagattcg ccagcgccac
    20761 cacctgctcg cgcatttccc gatagctgaa caggtaacgc gcatctgcca gcgccggagc
    20821 atgcggtgtt tttgccgctt gttctgccac cagcgcgctc agcgtggttt ctggaatctc
    20881 aacctgagtg gcgttgatct gcgccagctg cgcatattca cctggcagca taatatcgac
    20941 atcgccgcac aacagcgcag gatccgcggc gaactgggca atcagcattt tcaggcgttc
    21001 agcatgctgg attaacgttg gctcagcgta acgctgttta ttggcgagga tttcaatact
    21061 caaatctccg tgttcatccg ggaacagggc cagttcaagg tcattaaccg gaccggttgc
    21121 cagggtatgg gtttgcgcct gaacaccagg aatatccagt tggtaatcaa ataccttgat
    21181 attgagtacc ggaccgaaca gcggttcctc tcctgccgct cgcccactgt cacggacaat
    21241 ttgttcggcg tcgtagcgtt gatggcggcg catttttttc agttgtgctg ccagtcgggt
    21301 tgccagttgc ggcagcgttt cttgcgccgc aatgtgaata cccaacggta aaacgttgag
    21361 cacgggtccg gtagccgtca gcgccgccga gcccagtcga cgcataaaga taaatccggc
    21421 agcgtagtcc atgcggttgc acaatcgccc cagccacaaa gccgtcagcg caagggctaa
    21481 atcggtacgc tgcacacctg aaagttgcgt agccagctgg cggaattccc cgtcggtaaa
    21541 ttccagtttc aggcgcaaaa tatctgccga agcgctgcgc cccggtaaag gtgccggaga
    21601 aagtgacgcg ggcggtggca gttgacgacg ctgttccgcc cagaatgccg catcacgctg
    21661 ccaggcttcg ctttcgcggt attgctggta ctcttccact acatcagcga aaggcgtaaa
    21721 cggcgaagca ggcgttggtt cgccacgcag ccatgcgcag taaatattgg cgatctggcg
    21781 ggtaatggcc gggaaactga agccatcgac cagcaaatgg tgataacgct gataccagta
    21841 ccagcggtta tccgccacct gtatcaactg atgaaagacc agtggtttac cgctatcgac
    21901 gcgcagatct tgttgcaaat ccgcctgcat taatgactgc gcagtaccgt gcggatcaat
    21961 gttggttcgc aggtcgatag tttctggcag ttcgaacgtc agcgcatcat cgacccactg
    22021 ccagacttcg ccgttatctt ccgtaaaacg catccgcagc gtatctgctt gcgctaatcc
    22081 ggcaaccacc gcgcgggcca gtaatggcgc atcaacctct ccggttaact caacgtaatg
    22141 cgccacgctc caggcggagg gtaaatccga cagtttttct gccatccaga tgccgggctg
    22201 tgcggcgacc aaaggtaaat gctggctcat tgtgcctcct gcaactgggt gaaattcgcc
    22261 ggtgtcagag tacgccagtg ggcttccagc cactgctggc aggacacctg tgactgcggc
    22321 tgacacacaa cgtcccagcc tgccggtaag gcgcattgtt gcggccacag actgaattgc
    22381 ccctgcgcat tgcgcaaaat gtaaaacgct ccctgcggat catcgaaggg attactgaat
    22441 gccatattca actcctgtca tggaaaagtg gttgccagag gtcgattagc ccctgcatca
    22501 agccaccgcg ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt
    22561 cttttatggg gtgtaattgc gcatacagcg cctgattggc ctgcatgatc atcggctcgc
    22621 gaatacccgc ttccagcaca atgcgcagac cttcggcact aacttgacca gctttaagtt
    22681 gctcaagtaa aacaccttct tgctgcccgc cccgatgcgg ccaccagtac gatcctgact
    22741 ggcttaatac acagccaaag cgttcaggcc agtgcagtcc ggcatacagc gcggaaagcc
    22801 caccaaaact ctgcccggcg accacggtgc gatcggcgcg atcgctaaaa ggggcaatag
    22861 ctttcaccag gggtaataac tcttgctgta ctgccagcca gaaatccgca ttacacggca
    22921 gttcgtgggc gcggtgcgtg gtgtcaatag cgtcgatcaa cacatacacg gctggaggaa
    22981 gttgctgacg atgggtcaac gaagtcagca caggccagac aggcatactc tgcgcccaaa
    23041 attcgccatc gagcaaaact gccagcggac gttctcctgc tgttgcatcg ccagtggtaa
    23101 aaatccatac acggcgtgaa tttttcaacc gctcactttt ccagataatt tctttggcag
    23161 gtgtttctgg cgcttgcgga caatcccatc ccggttgtag gggcgcttgc ggcatttcga
    23221 gtgcagaaac agcgtgccct cgcccgcctt tccagctttg tggattcagc ggatcggcta
    23281 tcgcctgggg taatagtttt cgccagcctt cacgtaattc gaggcgatcg ggggatggtg
    23341 cagaaaaaat gtcatcgcgt tcggtgggaa taaaacagta gctgccgcgc cagttggcat
    23401 taagttgtgt cgtccactgc cagacgttgg tgcctgcaat tcgctgcatc gactggggct
    23461 ggctgttctg atggtgatcg gtcacaccag tgatgtagac ccatacgcgc tttatcgtcg
    23521 agtattcttc agaaccctgg ggatcacgcc accggaaagt gacctcaaac atttcgtcat
    23581 tcagacgctg ccattccggg ccatgtttcg actgccacca gctctcactt cctactttta
    23641 acgccgtcac accataaccc catgtttact gtgcaatttt tcattgattg cagaaatata
    23701 ttgataatat tattgataac tatttgcatt tgcaatagcg taatggcgcg ccgtgggaag
    23761 cgcggacatt aattaaccaa ctgcactgcg tgtctttcag gatcaaaggt tttcgcggta
    23821 gcgggatgcg tcgtgttgat gacgaccatg cccgacagtt gcaattcgtg gcaaaaatgc
    23881 aggaataaaa caatgaacaa gaagattcat tccctggcct tgttggtcaa tctggggatt
    23941 tatggggtag cgcaggcaca agagccgacc gatactcctg tttcacatga cgataccatt
    24001 gtcgttaccg ccgccgagca gaacttgcag gcacctggcg tttcgaccat caccgcagat
    24061 gaaatccgca aaaacccggt tgcccgcgat gtgtcggaga tcatccgtac catgccaggc
    24121 gttaacctga ccggtaactc caccagtggt cagcgtggga ataaccgcca gattgatatt
    24181 cgcggtatgg gtccggaaaa cacgctgatt ttgattgacg gcaagccggt aagcagccgt
    24241 aactcggtgc gtcagggctg gcgtggcgag cgcgataccc gtggtgatac ctcctgggtg
    24301 ccacctgaaa tgattgaacg tattgaagtt ctgcgtggtc cggcagctgc gcgttatggc
    24361 aacggcgcgg cgggcggcgt ggttaacatc attaccaaaa aaggcagtgg tgagtggcac
    24421 ggctcgtggg acgcttattt caatgcgcca gaacataaag aggaaggtgc caccaaacgc
    24481 actaacttta gcctcaccgg tccgctgggc gacgaattca gcttccgctt gtatggcaac
    24541 ctcgacaaaa ctcaggctga cgcgtgggat atcaaccagg gccatcagtc cgcgcgtgcc
    24601 ggaacgtatg ccacgacgtt accagccggg cgcgaagggg tgatcaacaa agatattaac
    24661 ggcgtggtgc gctgggactt cgcaccattg caatcgctgg aactggaagc gggttacagc
    24721 cgccagggta acctgtatgc gggcgatacc cagaacacca actctgactc ctatacccgc
    24781 tcgaaatatg gcgatgaaac caaccgtctg tatcgccaga actactcgct gacatggaac
    24841 ggcggctggg ataacggcgt gaccaccagc aactgggtgc agtacgaaca cacccgtaac
    24901 tcgcgtattc cggaaggtct ggcgggcggt accgaaggga aatttaacga aaaagcgaca
    24961 caggatttcg tcgatatcga tctcgatgac gtgatgctgc acagcgaagt taacctgccg
    25021 attgatttcc tcgttaacca gacgctgacg ctgggtacgg agtggaatca gcagcggatg
    25081 aaggacttga gttccaacac ccaagcgctc accgggacga ataccggcgg cgctattgat
    25141 ggcgtgagtg ccaccgaccg tagcccgtat tcaaaagcag aaattttctc gctgtttgcc
    25201 gaaaacaata tggagctaac tgacagcacc atcgtaacgc cggggctgcg tttcgatcat
    25261 cacagtattg tcggcaataa ctggagtccg gcgctgaaca tatcgcaagg tttaggcgat
    25321 gacttcacgc tgaaaatggg cattgctcgc gcttataaag cgccgagtct gtaccagact
    25381 aacccaaact acattctcta cagtaaaggt cagggctgct atgccagcgc gggcggctgc
    25441 tatctgcaag gtaatgatga cctgaaagcg gaaaccagca tcaacaaaga gattggtctg
    25501 gagttcaaac gcgacggttg gctggcgggc gtcacctggt tccgtaacga ttatcgcaat
    25561 aagattgaag cgggctatgt ggctgtaggg caaaacgcag tcggcaccga tctctatcag
    25621 tgggataacg taccgaaagc ggtggttgaa ggtctggaag gatcgttaaa cgtaccggtt
    25681 agcgaaacgg tgatgtggac caataacatc acttatatgc tgaagagtga aaacaaaacc
    25741 acgggcgacc gtttgtcgat catcccggag tatacgttga actcaacgct gagctggcag
    25801 gcacgggaag atttgtcgat gcaaacaacc ttcacctggt acggcaagca gcagccgaag
    25861 aagtacaact ataaaggtca gccagcggtt ggaccggaaa ccaaagaaat cagtccttac
    25921 agcattgtgg gtctgagcgc gacctgggat gtgacgaaga atgtcagtct gaccggcggc
    25981 gtggacaacc tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac cacgggcgat
    26041 ctggcaggag ccaactatat cgccggtgcc ggggcgtata cctataacga gccgggacgt
    26101 acgtggtata tgagcattaa tactcacttc tgatgctaac gtcagattgt tgacaaagtg
    26161 cgcgtaattc atgccggatg cggcgtgaaa ctgaactgca ccccaaatgt tggacataat
    26221 ctgacatctg aaggtgcagt ttatgaaaca ttcatttgaa gtaaaacttg ccgctgtaaa
    26281 tcattacctg gctggtcatg caggcatcat ttctacggca aaactctttc aactttctca
    26341 caccagcctc tcacactgga ttaatctttt tcttcttcac ggtcctcggg cactggattg
    26401 cagacacaag cgtagctatt ctcctgaaga taaactttgc gtggttcttt atgctctcgg
    26461 gcattctgag tctctacccc gggtagctgc ccggtttaac attcccagcc acaacatggt
    26521 taaaaactgg ataaaaggct accgcaaatc tggtaatgag gcctttatca ggcgcaggaa
    26581 ggagaaaagc atgacccgtt ctgatgatac ccatgaaaac gaggcaaaca tgacgccgga
    26641 agagatgaag aatgagctgc gttatcttcg cgcagaaaat gcatatctga aagccatgca
    26701 ggagcatctc ctggaaaaaa agcgccagga gctggagaaa aaacgaaagt catccggagc
    26761 ctgaagtgcg gacactgcca gtctgacctg ttaaaggctg cagggctggc cagaagcacg
    26821 ctgtactacc agctgtcact gcagaaggct aaggacaagt atgctgatgt gaaacagctc
    26881 atagcctcca tcttccatga acaccgggga tgttatgggt accggcggat ccattgtgag
    26941 cttcagaaaa gaggtcttaa gttcagtggc aaaacggtgc gaaaactgat gcaacagctc
    27001 ggcctgaagt ctcctgtgcg cctgaagaaa taccggtctt accggggaaa tatgggactc
    27061 gctgcagaaa atatccttca gcggcagttc aaagcggaag ctccctgtga gaaatgggtg
    27121 acagatatca ccgaattcag ggctggcgga caaaaactgt acctgtcccc gatacttgat
    27181 ttgtttaacg gggaaatcgt ggcgtgggaa actgcctgca ggcctacaga ggagctggtg
    27241 aagcgcatgt taaataaggg gctggaaagc cttgcagaag gagaaaaacc gctgctccac
    27301 aatgatcaag ggtggcatta ccggataaaa agttatcagt ctgccctggc agacaaaggg
    27361 ttagtgcaga gcatgtcacg caagggcaac tgtcttgata atgcggtaat ggagaatttc
    27421 ttaggtcacc tgaaagaaga aatgtactat cgccgggact acagaagtgt agaagagctg
    27481 gaaaatgccg ttaacgaata cataacttac tggaatcaaa aaagaataaa actcagtctt
    27541 gggggactca gcccgttaga ataccggact gagtatcaaa aagccggtta actgaaactg
    27601 tccaggtttt ggggttcagt tcagaacgcc ttatccggcc tacaaaaatc ttgccaattc
    27661 aatatattgc aggatagtgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg
    27721 tcatcagtct ctaatatggt cgatatgaaa actacgcata cctccctccc ctttgccgga
    27781 catacgctgc attttgttga gttcgagcca gcaagttttc acgaacagga tttactctgg
    27841 ctgccgcatt atgcgcaact gcaacatgcc ggacggaaac gcaaagccga acatttagcc
    27901 ggacggattg ctgctgttca tgctttacgg gaatttggct ttaagtacgc cccccctgtc
    27961 ggtgaacgtc gccagccact ttggcctgcc gggttatacg gcagtattag tcactgccag
    28021 gctacggcat tagcggtggt atcccatcaa ccaacgggta ttgatatcga agagatcttc
    28081 tctgcacaag cagcggaaga gttgaaagac agcattatga caccagcaga atacgagctg
    28141 cttgtggtca gcaatttacc cttttctgtg gtgcttacac tggcgttttc agcgaaagag
    28201 agtgcgttta aggcaagcaa gttacagaca gatcaaggtt ttctggatta tcagatagtg
    28261 aactggaata aacagcggat cattatttgt tgggagaacg agatatttgc tgtgcaatgg
    28321 cagataagag gaaaacaact cataacgctt tgtcaacacg attaatggat aacccttact
    28381 gtggttagcc agcaaccatt gcttgtagta gtcaagtaat attgaccacc gttttacgct
    28441 aagaacgtta tctgttctct aattttttcg gtgtcagccc accgttataa tggtgagatc
    28501 tgacgctatt atagttgttc aatatacaac cactgatttg ctgccatata tccggcgtcg
    28561 ttagtggact gatgacccgc gccatgatgg catccgcacc ctgtttccta acctatgtta
    28621 ccaccccgac accgttcagg ttgttacaga gtcgattatg cgatctgatc aatcgctgaa
    28681 tatcccaaat caccaaccgg acggagcagt gccgatcata gcaccaatac ccagaactga
    28741 acgacgcctg atccagaaaa cgatccataa aacaaaggac aaaaaaccac gcccggcggc
    28801 tcacagccat gctgatgctg catcgtggtg acaccgtcag tcatgctgcc agaacactct
    28861 gctgtgctcg ctcatcgatt ggtcgctgga ttaactggtt cacgctgtcg ggtgttgaag
    28921 gcctgaagtc attgccgtca ggacgtgaga ggcgctggcc gttcgagcat atttgcgcat
    28981 tgctgcgtga gcttgttaag cattctcccg gcgatggtta cctgcagccg aactggtatg
    29041 gcgcagagca gcaccaacac tgcgtatccg gggcccgaat aaggaagaaa aaatggcagc
    29101 gattaatgta gcgttagtca gttgtagcat agaacaccct gtgttttacg aagatgaagt
    29161 ggagatccac ctcaatccca aaatcggcgc agactggcag ttacgcgggc agcaaaagcg
    29221 cgttgttacg ccgggccgga atgaaaagta ctatcttgcc ggtgcgctgc acagtggtac
    29281 gggcaaagtc agctacgtgg gtggaaacag taaaggttca tccttattta tcaggctgtt
    29341 aaaacatctg aaggagacat actgacgggc aaaaaagatt acgctcattg tcgataacta
    29401 catcacccac aaaagctgtg aaacgttgcg ctggctgaaa gcaaacccaa agttcagggt
    29461 gatttatcag ccaatttact cgccatgggt caatcatgtc gaacggttgt ggcaggcgct
    29521 tcatgaaaca ataacccgaa atcaccagtg ccgttcaatg tggcaactgt tgaaaaaggt
    29581 tcgtcacttt atggataccg tcagcccatt ccccggtgga aagcatgggc tggctaaagt
    29641 gtagcggtat taggatcagc tacttagaag cgaatggagt aactggcata gcacagggga
    29701 atttaactac tcagcatata tatgcaagac ttgaataata tgtgggcgcg taaatagctc
    29761 ataaaaattt c
//
LOCUS       DAZGAP010000040        29453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGAP010000040 DAZGAP010000000
VERSION     DAZGAP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.040"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..512)
                     /gene="mocA"
                     /locus_tag="ACHLAM_001505"
     CDS             complement(<1..512)
                     /gene="mocA"
                     /locus_tag="ACHLAM_001505"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO7853060.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKIMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENA"
     gene            616..1386
                     /gene="yqeC"
                     /locus_tag="ACHLAM_001506"
     CDS             616..1386
                     /gene="yqeC"
                     /locus_tag="ACHLAM_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO7853061.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRSECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1434..3059
                     /gene="yqeB"
                     /locus_tag="ACHLAM_001507"
     CDS             1434..3059
                     /gene="yqeB"
                     /locus_tag="ACHLAM_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO7853062.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTECGFIPVIVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3100..4032)
                     /gene="arcC"
                     /locus_tag="ACHLAM_001508"
     CDS             complement(3100..4032)
                     /gene="arcC"
                     /locus_tag="ACHLAM_001508"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7853063.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4080..5465)
                     /gene="hyuA"
                     /locus_tag="ACHLAM_001509"
     CDS             complement(4080..5465)
                     /gene="hyuA"
                     /locus_tag="ACHLAM_001509"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO7853064.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQVPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5518..6729)
                     /gene="ygeY"
                     /locus_tag="ACHLAM_001510"
     CDS             complement(5518..6729)
                     /gene="ygeY"
                     /locus_tag="ACHLAM_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO7853065.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6787..7983)
                     /gene="dpaL"
                     /locus_tag="ACHLAM_001511"
     CDS             complement(6787..7983)
                     /gene="dpaL"
                     /locus_tag="ACHLAM_001511"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO7853066.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8041..9228)
                     /gene="ygeW"
                     /locus_tag="ACHLAM_001512"
     CDS             complement(8041..9228)
                     /gene="ygeW"
                     /locus_tag="ACHLAM_001512"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO7853067.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9707..11485
                     /gene="ygeV"
                     /locus_tag="ACHLAM_001513"
     CDS             9707..11485
                     /gene="ygeV"
                     /locus_tag="ACHLAM_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO7853068.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFISEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(11525..12004)
                     /gene="xdhC"
                     /locus_tag="ACHLAM_001514"
     CDS             complement(11525..12004)
                     /gene="xdhC"
                     /locus_tag="ACHLAM_001514"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO7853069.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITGIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12001..12879)
                     /gene="xdhB"
                     /locus_tag="ACHLAM_001515"
     CDS             complement(12001..12879)
                     /gene="xdhB"
                     /locus_tag="ACHLAM_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO7853070.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCQLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(12890..15187)
                     /gene="xdhA"
                     /locus_tag="ACHLAM_001516"
     CDS             complement(12890..15187)
                     /gene="xdhA"
                     /locus_tag="ACHLAM_001516"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO7853071.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAISINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            15603..16355
                     /gene="actS"
                     /locus_tag="ACHLAM_001517"
     CDS             15603..16355
                     /gene="actS"
                     /locus_tag="ACHLAM_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO7853072.1"
                     /translation="MSAGRLNKKSLGIVMLLSIGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKNSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            16434..16507
                     /locus_tag="ACHLAM_001518"
     tRNA            16434..16507
                     /locus_tag="ACHLAM_001518"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16466..16468,aa:Gly,seq:ccc)
     gene            16672..17886
                     /locus_tag="ACHLAM_001519"
     CDS             16672..17886
                     /locus_tag="ACHLAM_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7853073.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARAARDEAKRLIAGGVNPSDVRKAHKREQASKFGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            18014..18709
                     /locus_tag="ACHLAM_001520"
     CDS             18014..18709
                     /locus_tag="ACHLAM_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853074.1"
                     /translation="MPVVNDFPDLSHWFTVQEFTITQAALLLSGIDPLDYPTLEMVKE
                     EKHERWRMAYGFAYGMVTAIRRGVLTPVLCVAYDWGDMRRLTPAEMMDRSNNISMDGT
                     IITRYSLQEWVKNEKVDYVRPSRSISDKEIKPSNQSVIIDAESSVINSEETVLLTYGH
                     KSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILRPTG
                     LQQVGRRKRKTNS"
     gene            18957..19154
                     /locus_tag="ACHLAM_001521"
     CDS             18957..19154
                     /locus_tag="ACHLAM_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7853075.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLINQGRFPKPVKIGPRSVAF
                     IESEVDEWINQRIEASRNNAA"
     gene            19235..20011
                     /locus_tag="ACHLAM_001522"
     CDS             19235..20011
                     /locus_tag="ACHLAM_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7853076.1"
                     /translation="MPVQGQGFVQPEELSQQYFAAIIPVISGNEIIADGINKNALTAP
                     EVFPSGGEPVTTSQAIARFFRKDHDKVVNRIENLCCSAEFLTRNFRRVKFEHKGRVLK
                     AYEVTKDGFVFLTMGFTGKKAAAFKEAYIAQFNRMANWIAERCEAKAEMRSMGDAMKG
                     YIERTGDTQHGHAYNNECTFIARLVLGMHPPVWAKKNGIPSKKVRDHMTADQLALLAY
                     LESRNCALLDMDMPTPERKERLTELAQRWLVRRLGEREHA"
     gene            20004..21905
                     /locus_tag="ACHLAM_001523"
     CDS             20004..21905
                     /locus_tag="ACHLAM_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO7853077.1"
                     /translation="MPDILKPAVRYIDTADKFALLNINHADYMRHIVSHFTAERMKHV
                     KQYLEEGLSNYRAGGPVRREYPHDAGMAGKTKATTKGSPVEIKEIKRIIHNAISEAGG
                     QYAVYDEWPLGPDVRIFLRHVLFRKLSSAKASGASLCSRARALSSLRVNSVALACSRA
                     AFRFRSCSVIRALVSVTIMAKEFFFWSSISYPLLLDRLECCFKKSSSAKLVLKVFSNR
                     LRISISAMCLVIDCFFSTKCCSYAVRSSFLFSDCEVCCVLSILCTCKTMRRTSSHHGA
                     DGFCLFHRAAEETASYQPGGRFLVAFNRRGYFSTKASRANRVGLVSGCGLSNNLLCRC
                     AFSRCRSSMDNAISCSSSFVIVCTCKAIRRNTPRYSDDGFCCCNLLRWFFLPLRNTSS
                     GGGSLVFSGGVNFSFLVSMMSRISRWRCSALIFGVTGENLCLLILDSGNALCRDASYH
                     GAGYCLVRCLSFWYPSFLEGYVNIFRTNFSRNTSDHLSKKAAYTEAIPYKTGIGIGLP
                     LELEAIHDAPASFFVSAHTHTSTMVGCMELPSGRLVSSNTSKANSVQSTASKIGLLGG
                     GYNPKVRGAFHMAVSARPYFVWRFMQCLTDSIAIFTVTAATEREARAQLPHAHLIFVA
                     RIRQEVTHA"
     gene            21898..22083
                     /locus_tag="ACHLAM_001524"
     CDS             21898..22083
                     /locus_tag="ACHLAM_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853078.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            22087..22281
                     /locus_tag="ACHLAM_001525"
     CDS             22087..22281
                     /locus_tag="ACHLAM_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853079.1"
                     /translation="MCNFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            22348..22461
                     /locus_tag="ACHLAM_001526"
                     /pseudo
     CDS             22348..22461
                     /locus_tag="ACHLAM_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            22467..24287
                     /locus_tag="ACHLAM_001527"
     CDS             22467..24287
                     /locus_tag="ACHLAM_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO7853080.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHSERQNWRNYLDIERKNISDGLVIELPVKKKARGQGDDSSLALNQMGASQ
                     RGEVLLAHYGGELAINADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDICYSQNAI
                     KSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPAE
                     GETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKS
                     VMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAITG
                     GDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENERD
                     PMLPEKIEGELAVVIRHLLTRFSDQDEARLLLHEQQKSEEALAIKREGDSLVDFCGYL
                     MSSVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFSKPVTLTRFGADMPGAMAEY
                     GREYMKRKTKHGLRSNVTLTEESEDWMPSCAPVKNEGDKN"
     gene            24574..25023
                     /locus_tag="ACHLAM_001528"
     CDS             24574..25023
                     /locus_tag="ACHLAM_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853081.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAGKIYEKYAANS
                     KRAARAITYHFMTLYDLKADDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            25020..25469
                     /locus_tag="ACHLAM_001529"
     CDS             25020..25469
                     /locus_tag="ACHLAM_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7853082.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            25486..25932
                     /locus_tag="ACHLAM_001530"
     CDS             25486..25932
                     /locus_tag="ACHLAM_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7853083.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKVQRKNHRRINRIAEYWPE
                     LFGTENIKPLKAGIIRDVLEDAKSRELPLATGVLRGALTSHLHTTRYLKAIIAGGPRY
                     DLNGQPCGEVTEEDKSVASELLKRRLAQIKQQNSQQQTTGDDGDDS"
     gene            25922..26215
                     /locus_tag="ACHLAM_001531"
     CDS             25922..26215
                     /locus_tag="ACHLAM_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7853084.1"
                     /translation="MIADSVAQKLEERGLWRRAATRWGDVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQLVTFGDVKKAADRTLKEMGINAQDEWKNYSFSDAGDDLALP"
     gene            complement(26652..26849)
                     /locus_tag="ACHLAM_001532"
     CDS             complement(26652..26849)
                     /locus_tag="ACHLAM_001532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853085.1"
                     /translation="MIGFVIGFVIDLRIFLRNFLCIFSGIFPWIFHPTEGASGLPDND
                     QVNRTGKGAGCAGGRAIKNPE"
     gene            27126..27339
                     /locus_tag="ACHLAM_001533"
                     /pseudo
     CDS             27126..27339
                     /locus_tag="ACHLAM_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28410..28784)
                     /locus_tag="ACHLAM_001534"
     CDS             complement(28410..28784)
                     /locus_tag="ACHLAM_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853086.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
BASE COUNT         7579 a         6897 c         7594 g         7383 t
ORIGIN      
        1 tctgcatttt ctatttcgac ggaataatgt tctcccataa ggagtgcctg acgcatattg
       61 gtgacattgg gtcgctggat tgcctgcatc aggcatggtt ttgacactaa aatcggatgg
      121 ccggggatgc cattatggag tggcagtatt gcgccatcat ttcgtaacga ccagattttt
      181 ctaaaaatat cgatggtgag ggttggcata tctccgtggg tgagaaaaca atgttctgtt
      241 tgcaccgcgg gtactgcggc ctttactgat gtcagtaaac cctgcgcata atctgggttg
      301 tggataatag tgatattgct ctggttcgca taacgttcgt gcagttcatt accacgatag
      361 ccggtgacta aaataattcg gctacaaaac tgcaacgcat ttttgatact tgtatcaaga
      421 attgttccct gttgccaggg taacataatt ttccattgcc ccattcttga tgataatcct
      481 gcggcggtaa ttatacagtc gatggctgac atagtgattc cattatttac aggagctaat
      541 aacgcctgat aattgcgtta cttcctgatg gattaagttg agggtaattt atcccagaga
      601 ggtccataaa gacgcgtgaa aagtataatt gacccatcgg cattattcat tgatttaggc
      661 gcgcagaaac gtcccactgt gatttctgtt gttggtgctg gggggaaaac cagcttgctt
      721 ttttggctgg cagaactgtt ccaggcgagc ggcaggcgtg tattaatcac tacgacgaca
      781 catatgttta tgccaacatc tcactggccc gtggttttct gtcgtgatcc cgccatgctt
      841 cctcatgcgt cttttatatc ccccatttca ttttgttttc acagctggaa agcgaaccag
      901 ggaaaagtgc agggatttac gccagaagcg attgatgcac tggtacaacg atcagagtgt
      961 gacgtaattc tcattgaggc agatggctcg cgtggaatgc cgttaaaagc gcctgatgag
     1021 cacgaacctt gcatacctaa aagcagttgc tgcgtgattg ctgtgatggg aggacatatt
     1081 ttgggtgcga aagtgagcac agaaaatgtc catcgctggt cgcagtttgc tgatattacc
     1141 gggttaacac ctgatgcaac cttgcaactg agcgatctcg ttgcgctggt tcgccaccct
     1201 cagggggcgt ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat taaccgtttt
     1261 tctcaatgtg agaatgcgat tgcgcaaagc gagctccttc aaccgctgca acaacacgac
     1321 gtagaggcaa tctggctggg cgatatacaa gaacatcctg caatcgcgcg cagatttgtg
     1381 aattagcggc ccgggtattc cgggttactg aaaggatatc cattgaggtt ggtatgaata
     1441 ttttcacaga ggctgcaaaa ctcgaagagc aaaattgtcc gtttgcgatg gcgcaaattg
     1501 ttgatagccg aggctcaacc ccccgccatt ctgcacaaat gttagtgcgc gccgatggct
     1561 ctatcgtcgg tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa gagtcgcttc
     1621 aggcattgca ggaacgtaag ccgcgattat tccatggacg tatggctcgt aacggtgcgg
     1681 atgctgtcgg gtcagattgt ggaggtgcaa tgtcagtatt tatcagcgtc catggtatgc
     1741 gtccacgtct ggtgttgatt ggcgcggggc atgtcaaccg ggcgatagcc cagagtgcgg
     1801 cgctattagg atttgatatc gccgttgccg atatttatcg cgaaagcctc aatcctgaac
     1861 tattcccacc atcaaccacg cttctccatg ctgagtcgtt tggtgcggca gtggaagcac
     1921 tggatattcg ccctgataat tttgtcctga ttgccacgaa taatcaggat cgtgaagccc
     1981 tcgacaaact cattgaacaa cccattgcat ggctggggtt gctggcaagt cgccgcaagg
     2041 ttcagctttt cctgcgccaa ttgcgtgaga aaggcgtggc tgaagaacat attgcccgtt
     2101 tacatgcgcc cgttggttac aacataggtg cggaaacgcc gcaggagatc gccatcagcg
     2161 tgctggcaga aatattacag gtgaaaaata acgcgccggg tgggctgatg atgaagcctt
     2221 ctcatccttc cggacaccag ctggtggtga ttcgtggtgc gggggatatc gccagtggtg
     2281 tagcgctacg tctgtatcat gcgggtttta aagtgatcat gctggaagtg gaaaaaccga
     2341 cagtgattcg ttgtaccgtg gcatttgccc aggccgtgtt cgatggcgaa atgacggtcg
     2401 aaggcgtcac cgcccgcctg gcaaccagct ctgcggaggc gatgaaactt accgaatgcg
     2461 gattcatccc tgtgatagta gatcccgcct gttcattgct tgatgaactg aaaccgcttt
     2521 gcgtggtgga cgctattctg gcgaaacaga atttggggac gcgggcagat atggcaccag
     2581 taacaatcgc gcttgggccg ggctttactg cagggaagga ttgtcatgcg gtaattgaaa
     2641 caaatcgcgg gcactggctc ggtcaggtga tttactctgg ttgtgcgcag gagaataccg
     2701 gtgttcctgg caatattatg gggcatacca cccgacgggt gatccgtgct cctgctgcag
     2761 gcattatgcg atccaacgtg aaattaggcg atctggtgaa agagggcgat gtgattgcct
     2821 ggattggtga gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc ttgttgaacg
     2881 acggactggc agtggtcggt ggttttaaaa ttggtgatat cgatcctcgt ggtgaaacgg
     2941 ctgatttcac cagcgtttct gataaagccc gggcgattgg cggcggcgta cttgaggcgt
     3001 taatgatgtt gatgcatcag ggcgttaaag cgacgaaaga agtgctggaa gtggcttaaa
     3061 tgaaaatggc cggatggtat tacccacccg gcctttaaat tactgacaaa tcaccgttcc
     3121 ggttttgcct tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg cttcacgacc
     3181 tgcgcgagag cgagcaaacg aggcggcagc ttccacttta ggcagcatag aacctttcgc
     3241 aaaatgacct tcttcgataa agcgttcagc atcacttaat gacaggcgat cgagccactg
     3301 ttcattctct ttaccgaagt taatggccac tttttctacc gcagtcagaa tgatcagcat
     3361 atcggcatca atcatttctg ctaaacgggc gctggcccag tctttatcga taaccgcact
     3421 ggcaccgcgc agatggttcc cttcacgaat aacgggaatg ccaccaccgc caacggtgat
     3481 cactacctgg cctgcttcta ccagcgcttt taccgtctct ttttcaataa tatcaaccgg
     3541 tttcggcgag gcaacaacac ggcgatagcc gcgaccagca tcttctttca gagtgtaacc
     3601 ctgttttgtc agttgctctg cttcctgctc ggtaaagaac gaaccgatcg gcttggtcgg
     3661 gttgaggaat gccgggtcgt tagcatcgac ttcgacctgg gtaaccagcg ttgctacagg
     3721 tttattaatg ccacgagaaa gcagttcttc tcttaatgcg ttttgtagat cgtaaccaat
     3781 ataaccctgg cttaaggcaa cacagacaga catcggcagc attggcgagt gcgcttcagt
     3841 ttttgcggca gcttcaaagg cctggttaat catgcccacc tgtggcccgt taccgtgagt
     3901 gacgataact tcatgtccct gggcaattaa atcaacaatt gcctgagaag taatttttac
     3961 cgctttcatt tgtccggcca ggtcatcgcc cagcgcattc ccgcccaggg cgagaacaat
     4021 tttcttactc atttttcctc actcatcatt cttgattgtg tatagatccc tggcagtggt
     4081 tagagcacgg gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc ggctttgcct
     4141 gtaaaggtgc cgtcacagaa aatcgtttca ccacgggata aagttctgac aatcgcgccc
     4201 tgacaggtaa aaccctccca tggcgagtag tcggcgttgt cgtggagatg gcgatgctga
     4261 atttgttgac tctgacgtgg gtcgataatc accacgtcgc catcggaacc gggcgctaat
     4321 aatccttttt gcggccacag gccaaacagc ctggcgggca tcgcgctggt taattcaaca
     4381 aaacgttccg gtgttatacg tcccgtcata acgccactgg aaaacagtaa ctgcatgcgg
     4441 ttttccacac cgggtaagcc atttgggcag cgactgaaat cgcctttaga aatttgcagg
     4501 cgttgagcca tcgagaatgt gcagtgatcg gtcgctacca cgtcaatcgc accatcgctg
     4561 atgccacacc acagtttgtc ctgctcgcgt acgttacgca gcggtgggct aagaatgaac
     4621 ttcatgccat cttctgtatc gtaactgcgt tcgtccaaca ggagatattg tgggcaggtt
     4681 tcaacccaga ctggctggtg attcgcacgg gcaagacgca gataatccag acctaagccg
     4741 ttagacaggt gcacgatata gagcggggcg ttaccggcaa tttgtgccag gttaatcatg
     4801 cgggcgatgg cttccgcttc gcattccaga ggtcgactca aggcatgata acgcggcgcg
     4861 gttaaccctg cggcgataaa ctccgcccgc ttgctggcga tagctgcgtc attttccggg
     4921 tgcacggtgg tcagcgcgcc ggattcatgc aagcggcgta atgcctgcaa cacttcatca
     4981 tcgttgagtt tatattgata ggttaaatag agtttaaaac tgctcagtcc ttcctcaacc
     5041 atcatcggaa tttcgtcgag gatagcgtga ttgatatgtt gaatgacacc gtgaaagctg
     5101 tagtcgatga ctgctttgtg ggcggcataa ccgcgataaa cttccagttg atggcgtaac
     5161 cggcagccat tcgggccaaa tcccatatgg tcaataatgg ttgttgtacc gccacacgca
     5221 gctgcgcggg taccggtaaa aaaatcatca caactgcgcg cgatgccgac atcaatattg
     5281 aaatgcgtat ggacatccac gccgccaggg aaaacgtaac agccagtggc atcaatttct
     5341 tcatacggga cctgcggcga aatattgctg cccaattggc gaacaatccc gctctcaatc
     5401 agcaaatctt gtttggcttg tccatctgcg ttaacgacag tgccgttttt gatcaatacg
     5461 cgcatagcaa actccaaaaa ccctccggcg aaccggaggg gaataagtga agagtaatta
     5521 ttcggttgcc agccagctta acgggattgc agcgtacatc gcggcacagg tcaccaggtg
     5581 agatttccag gttttttcgt taggtgcatg cgcttcaggt tctttacccg ggccaaagcc
     5641 gatcaccgga atgccgtgac ggcccatgat agatacgccg ttagttgaga aggtccactt
     5701 atcaacaacc ggcgctttgc caaacagacc ttcgtaggca ttcaccagtg ctttaacggt
     5761 gaagtgatct tcttccactt tccaggtcgg gaagtagcat tcggttgggt aaaccaggcc
     5821 agtccaggac ggacggtcgt agttgtacat agaaacaacc gcgttagctt tctgtactgc
     5881 aggcagggcg cggatttcgt ccagcgcgcc ttcccaggtt tcgccccagg tcagacggcg
     5941 gtcaatagag acggcgcagc tatctgctac agcgcaacgg cttggggatg tgaagaagat
     6001 ttcagaaacg gtgagggtgc ctttgccgag gaattcgtca taacccagac gttgggagag
     6061 ttcttgtaat tcgccaagaa tcggacccat tttgaaaatg gcgttgtcac cgcgttctgg
     6121 tgcagaaccg tggcagctca caccctgaac atcaatacga atttccatac gaccgcgctg
     6181 accacggtat acctggcagt cggttggttc ggtactgacc acaaattccg ggcgaatgcc
     6241 ggattgttca ataatgtact gccagcacag gccgtcgcag tcttcttcct gcacagtacc
     6301 ggtaaccagc agggtatatt catcttccag accgaggtct ttaatgattt taccggcata
     6361 aaccatagat gccatgccgc cttcctggtc ggaagtaccg cgaccaccga tcagctcatc
     6421 agtttccatg ccttcgtacg gatcgaagtc ccagtttttg atgttgccaa tgccgacggt
     6481 atcgatatga gcgtccattg ccaccagacg cgggccgtgg ccgatataac cgagaacgtt
     6541 gcccatcggg tcgatttcaa ctttatcgaa gccgactttt tccatctctt ctttaatacg
     6601 atgtactacg cgtttctcgt cgcagctttc actgggaata gcaaccatat cgcgcaggaa
     6661 gcgagtcatg tctgcctggt aatcttttgc tttttcaaga atcagtttga atggaatatt
     6721 cttagccatt atttttctcc agatttttta ctccccgttt tgcagggagc agttccaata
     6781 gggtgattaa ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt agtgcttcac
     6841 gtcggtgtcg ccttcagtgc tgataaccag caccacggca tctttgttca gcgccagttt
     6901 ttccatcagg ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc ccaaaccgac
     6961 agcgccggat tcaccggaga tgatgcgcgg gtcgttgcca tacggattac ccagcacgcg
     7021 catacctaat gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc agttacgtag
     7081 gatttcccag cccagtgggt taggttcgcc gcaggccagg cccgccatga tggtggccat
     7141 atcaccgcca acgttgacga tgtcgccttt gacgccggag cgataaatac agtcagcttt
     7201 gtcaggttca acaataatgc tgtgcagatt ttgcgggcta tagacgtcga ccagataacc
     7261 cagcacacca ccggccattg caccgacacc ggcttgcagc agaacgtgtg tcggggtgac
     7321 gcccatttca cgcatttgct cgacggcttc atccgccagt gttgcgtagc cttgcatgat
     7381 ccaggttggg attttggtgt aaccttccca tgccgtgtcc tgtaccactt cccagccgtg
     7441 ctgctgcgcg tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat ccgtgacgat
     7501 gcactcggca ccgaggttta gaatggcgtc aacgcgttcc tgagcagaac ctttcggcat
     7561 gtaaatcacc gcattctgtc cgagttgctg tgctgcccat gccacaccgc gcccgtggtt
     7621 gccgtcggtg gtagtcgcga aagtcatttt ttcgccgatg gcatttttca ggtgctcaaa
     7681 tgacagcgtt tcgatatcaa gatgatattt ttcacacaat aattgagcga tggcgtacgc
     7741 accgccaagc attttgaagg cgttcagacc gaatcgtttt gattcgtctt tgacgagaat
     7801 tttctttaca ccaaacaggt ttgcgagatc gtccagcgca caaagcggtg ttgggtgata
     7861 accagcaatt ttctggtgga actggcgtgc cagttttgcc tggctttgcg aaaaaagcgg
     7921 tgatgtttcg ccgttgaaaa atttgttatc ggcgatatca atcttcaatg agaaaacgga
     7981 catatcctat ccttttggcc tctggaaata gagggccgac cagctgatcg gcccgagggg
     8041 ttatttcacg cgttcttgcg cttctttcag cagttgctcg agcagtgcac ctggtttggc
     8101 gtatttacgg gacaggatca tcgcggcgat gatataaggc ttccagctgg cttctttgta
     8161 ggtagcgata cggtattttt cgaatacgcc ttcagtcact tcaccttctt tgcaggatac
     8221 gccgctgata tcagctggca ggcagtgcat gtacagggct tcgccatcac gggtcagttc
     8281 catcatctct tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca gacactgttt
     8341 ttccagtgct tttaagcctt cgtgatcgtt cgcacgcagc aattcagtac gctcttccat
     8401 cactttgtaa ggtgcccatg acttcggata aacgatgtct gcgtctttga aggcttcttc
     8461 catgctggtg acctgacgga agctaccacc ggaggcttta gcattgtttt tagcaacttc
     8521 aacgacatcc gggatcaggt cgtagccttc cggatgggcc agggtgacat ccataccgaa
     8581 gcgggtcatc agaccgatga tgccttgtgg tacagagagc ggtttgccat agcttgggga
     8641 gtaggcccag gtcatggcga tttttttgcc tttcaggttt tccagcgaac caaagtgttc
     8701 acgcaaccat gccaggtcag ccatagactg agtcgggtgg tcaatatcgc attgcaggtt
     8761 cactaaagcc ggacgctgtg gcagaacccc ttgtttgtag ccgtcatcaa gtgccgcgcc
     8821 aacttcacgc atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa taccgatggc
     8881 atcggcgcag aaggagatca tattggcggt ttcacgcacg gtttcgccgt gagcgatttg
     8941 tgatttgcct tcgtcgagat cttgctgtgc aaggccgagc aggttaagcg cggaagcata
     9001 agagaagcgg gtacgggtgg agttgtcgcg gaatacggaa atacctaatc cactattaaa
     9061 gactttggtt gagatgtttt cagcacgcag tgcttttaat gctgcggcaa cgtccagtac
     9121 ttgtttcagt tcatctggcg tctgttccca cgttaacaaa aaatctttct cgtgaaggtg
     9181 agaggtcagc gaattgatat ccttaatcag ctcattaaca gttttcatca taaagtcccg
     9241 gtagtggaat gacaggcaaa gtggtttatt tcactgtgac ttgccaacgt gattggtgtt
     9301 ttcacagcta tacctgggac taacaataag tgtgccagaa ttatccacgg ggctgcaaaa
     9361 gactaatttg acggataatg tgtggggttg atcacaaatt gatgccagct tctggttgtt
     9421 tgtaaaatac gaaaacgttt ggcttttttt atcgcgttat ttatttgctg ctaagtatca
     9481 gaaataagat aaagggacag gcgatctctc ttcagtttga taaactcacc gccgtaattc
     9541 tcatattgat atagaaatgt gatatctctt ccataatttc attagaaagt gataaaaatc
     9601 ttatgttttt atcaaagcca atttcgcctg agttaatatc atcatgatct atgaaccatc
     9661 aacgtcttca atgatcgact tttaagtaag tctggaaggt aaagctatgg agcttgctac
     9721 tacgcagtca gtattgatgc aaattcaacc gacaattcag cgttttgcca gaatgcttgc
     9781 cagcgttttg cagcttgagg ttgagatcgt tgatgaaaac ttgtgtcgcg tcgccggaac
     9841 gggcgcgtat gggaagtttc ttggtcgcca gttgagcggc aactcacgcc tgctccgcca
     9901 cgtcctggaa acgaaaactg aaaaagttgt gacacagtct cgcttcgatc ccctttgtga
     9961 aggttgcgat agtaaagaaa attgccgcga aaaagcattt ctgggtacgc ctgtcatttt
    10021 acaggatcgt tgtgttgggg tgataagttt gattgccgtt acccacgagc aacaagaaca
    10081 tatcagtgat aatttacgcg aattttctga ttacgttcgc catatatcca ccatttttgt
    10141 ttcgaaactt ctggaggatc aggggccagg agataacatc agtaaaatat tcgcgaccat
    10201 gatcgataat atggatcagg gcgttttagt tgttgatgac gaaaatcggg ttcagtttgt
    10261 taatcagact gccttaaaaa cactcggtgt tgtgcaaaat aatattattg ggaaacctgt
    10321 tcgtttcaga ccattaacat ttgagagtaa ttttacccat ggacatatgc agcatattgt
    10381 ttcgtgggac gataaaagtg aattaatcat tggtcaattg cataacattc agggccgaca
    10441 attattttta atggcgtttc accaatcgca taccagtttt tctgtagcaa atgcacctga
    10501 tgaaccgcat attgaacaat tggttggcga gtgccgtgtt atgcggcaat taaaacgact
    10561 cattagccgt attgcaccca gcccatccag cgttatggtg gttggcgaaa gcggcacggg
    10621 taaagaagtc gtcgcccgtg caatccataa gttgagcgga agacggaata aaccttttat
    10681 tgctatcaac tgtgccgcga ttccggagca gcttctggag agcgaactgt tcggttatgt
    10741 taaaggcgca tttactggcg cttctgccaa cggtaaaaca gggttgattc aggcggcgaa
    10801 tacgggcacg ctgtttctcg atgaaatagg tgatatgcca ttaatgttgc aggctaaatt
    10861 actgcgcgct attgaggcac gtgaaattct gccgattggt gccagtagcc caatacaagt
    10921 cgacattcgc atcatttctg caactaacca gaatttggcc cagttcattt ccgaaggtaa
    10981 attccgcgaa gatctcttct accgacttaa tgttatcccg ataactctgc caccgctgcg
    11041 tgaacgtcag gaagatattg aactactggt gcattacttt ttacatctgc atacccgtcg
    11101 tctgggatcg gtttatccgg gcattgctcc cgatgtcgtc gaaatattgc gtaagcatcg
    11161 ttggcccgga aacctgcgcg agttaagcaa tttgatggaa tatctggtta acgtggttcc
    11221 ttcaggtgaa gttatcgaca gcacgctatt gccgccaaat ctgctgaata atggcacaac
    11281 ggagcaaagt gatgtaacag aggtcagtga ggcgcacctg gcactcgatg atgcgggcgg
    11341 cacggcgctg gaggagatgg aaaagcaaat gatccgcgag gcgctttcac gtcataacag
    11401 caagaaggaa gttgctgatg aactgggcat cggcattgct acgctctatc gcaagattaa
    11461 gaaatatgag ttgttaaaca cataacctca gtgaatccgg cctgaattca ggccggatat
    11521 ccttttactt cgttttctcg caatccagaa ctgtatttac aatcatctga taccccgtgc
    11581 agcgacaaag atttcccgcc agtccacgac gaattcccgt aatggttaat ggcttctcgc
    11641 gtggtttcgc cagcattgcc gtggtagcca taatcaggcc cggcgtacaa aacccgcact
    11701 gcactgcgcc ggatttcgca taagcctgct gaacatgaga aagttttccg cctttcgctt
    11761 caccttccag cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat aagcaactgt
    11821 ctattgctgt gccgtcgacc agcaccgtac aggcaccaca ttcacccacg cagcaccctt
    11881 gtttgacact tagcagtcct tgttcgcgga gtaattccga gagcggcgtg cctggtgcgg
    11941 cgtgaagctg aaaaggtatc ccgttaatgg tgcattcgat ggtaattgtt tcgctgtgat
    12001 tcattgcaat tttccccccg ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt
    12061 ctggatgaga tgcagacgaa actctttact ggcccgccat gacgaacgcg gggcgacatc
    12121 ttgcaggact gattcgctga tggcttccag cgtttgcagg tttaatggcg cattttgtgc
    12181 agtctgttcg gcatgttggc agcgaattgg cgttggcgcg gcaacaccaa atgccaggcg
    12241 taattcgctg aaattgccgt tatccagttg gcaatgtgcg gcgcagccaa tcgttgaaat
    12301 atccattgcg tcgcgcatgg catatttaaa atgcgcgctg cccgcgtgtt ctttcggctg
    12361 gggcggaaaa tgaaaggcga caaggatttc gtcatgctca agagacactt tgcccggccc
    12421 ggtgtgaaag ccattaatcg ggacgaaacg aacaccgcgc ggggagtgga tctccagttt
    12481 cgcgtcataa attagcgttg gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt
    12541 tccaccgtag gtagcgacgt tacggatctg cggtccggca atggatgagg ccgcagcaca
    12601 taacgccggg agatgacgtt gagttacagg atcttcaatg agctgggtaa atgtcgttgc
    12661 agagccgata cgtagtgagc catcttccgc cagcgtaatt ccccgcagct ccgccagatt
    12721 atgaatatca acaatatggc gataacggtc attgtgatgg tggagctgaa tcagtacgtc
    12781 agtgccaccg gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga tggcatcggc
    12841 aagggttgct gcgcgatggt aagaagcaaa atcaaacatg atgttatcct caaatcaatc
    12901 ctgccagatg gaactcttca tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa
    12961 caccggtagc catcttcacc gcgttacgaa tagcagcggc aacaggaatt attggtggct
    13021 cacccagtga cttatgtccg tatgcggatt gcggctcatt gatttcgacg aacgcgcttt
    13081 ccagttgtgg cagatccggc atggtcggca ttttgtaatc cagcagattg gggttacgga
    13141 ccacgccgct tttcgcatca atgatcatct cttcaaatag cgcccagcca atgcccattc
    13201 ccattccgcc gtgtacctga ccttctgcca gtagcggatt aagaatatgg cccgaatcat
    13261 gaacgttgag gatgcggttg atggtgactt tacacagcgc aatatcgacc gtcagatcaa
    13321 caaaggtaca gccaaacgct ggtgggttag tggtggtttt gatggagctt tcggcagaga
    13381 gctgcccgcc gcgttcaggg tggtagaagg cgtccatcgc caaatctttt aacgacatta
    13441 atggctcttc cggtcgttca accagcacga tatggccttt tatcagggta agattcatcg
    13501 ctgactgatg tagcatgaca gcggcatgag cgatgatttt ctcctttaat aacagtgccg
    13561 cactgcgcag cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgca ccgggatcga
    13621 acggcgtaac gtcggtatct tgggttgaaa taacgcgaac gtcgctgacc ggaaccccca
    13681 cggtttctgc taccatttgc gagaagacgg tgtcggcccc ctgaccgatt tctgtcgcgc
    13741 cgctttgcac gttgatggtt ccatcctgat tcatcaacag gcgcgcgcct gctatttcta
    13801 cgccgacagg ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg ccgcggcgta
    13861 aattgccttg ctggttctgg cactctgcac ggcgtttttc ccattcaaag attttccggc
    13921 ctttttcaag acactccggc aaccctgcgc tgtaaatacg tttgcccgtg agcggattag
    13981 catctccttc gcgggcggcg ttgcgtaaac gaatttcaac aggatcaata cctaacgctg
    14041 tcgcggcgtc atcaagcata gactcaacgg caaatacgac ttgtggcgcg ccataaccac
    14101 gcatcgcacc agccgagggg aggttggtat agcaggtctt tgaactgtaa gcgtaggcac
    14161 aacgaggata aaggtaagcg actttatttc cgcccgcaga agcgatggag tgcccgtgag
    14221 atgcataagc gccggtgtta gacagaacat ccagactata acctttcaat gttccgtcgc
    14281 ggttcacgcc catttgcccg tcaatagtaa aagcgtggcg ggtacgggtt gcgaggaaac
    14341 actcttcacg gctaagggaa actttcaccg gaatgccgcc aagcttgctg gtcaggaatg
    14401 ccgccattgg ctcttccagt acatcctgtt tattaccaaa accgccaccg acaaacggtt
    14461 tgatgactcg tacgcatgac cagggaatat tcagcgcctg accaaccaca cggcgaacaa
    14521 tgtgcgggat ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc atccatgcca
    14581 gcgatgtcac gctttccata tgacaatgtt gaataacggg agtctgatag tgcccctgca
    14641 cctggtaatc ggcggcatcg attgtttgtt ggacattacc cgtcgacatc gagctttgtt
    14701 ttagtaaatt gccgccgtta tggattggtg cagcgtcatc tgccagcgcc gcttctggcg
    14761 tggtgataac gggtaattct tgccactcaa tgctgaccaa ttgcgccgct ttttctgccg
    14821 tgagttcatc acgggccacg acgatggcaa cggcgtcacc atgatgacga acatggcgag
    14881 ttagcagtgc gcgatcggcg gtatcgcgct tgttttcgtc aagtgtccag gcatgcccag
    14941 ctgtagcgaa tgggatatca ggcacatctt cccaggtaaa aatcgccagt acgcccggta
    15001 aacttctggc ttgttcatca ttaatactta tggcataacc atgtgcgata gggctacgta
    15061 catatttcgc gtaacacatg cccgccataa cataatcgtc agtatatcgt gcccgcccgg
    15121 tgaccttagc aatggcatct acgcgcatgc atgattcacc tgtagcggtt gcttcccgcg
    15181 cttccataga aatcccctcg atttgaacat ttggttctta tggcaaagat ttagtctaag
    15241 caggcaagat ttacgccaga aacgacgata agcgttaagg gaggaaggaa gtgtgatgat
    15301 gttaaaaaaa cacagatatg atacgaaaca ggaccctgaa taggggcagt gatacgtatc
    15361 aatatgagtg gcgataaaat caatgtgata aattgggatt attacgcatc aggatgggaa
    15421 atagggtaaa tgagtcagaa aattcgatac atctacagtg aatattttgc tgatgatact
    15481 tccctttgcc aaatgaaatt aacctctttc tctattaccg cgtttttcca gaagcaagag
    15541 attgggtgtc aagaggctgg ctgctatgat atggcgtctt gttttttaag cgaggaaaga
    15601 ttttgagtgc gggacgcctg aataaaaaat ctctgggtat cgtgatgttg ttatcgattg
    15661 gactgctttt ggcgggctgc tcgggtagca aatcatccga tacaggaacg tattccggct
    15721 ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc acgggaacta
    15781 gcgtaaaaga gctggcgcga ctgaacggca tttccccccc ttacaccatt gaagttggtc
    15841 agaaactaaa actgggtggg gcgaaaaata gtagtacacg taaatcaacc gccaaatcaa
    15901 cgaccaaaac cgcatcggtt acaccgtcat cagcagtacc gaaatcttcc tggccgccag
    15961 tagggcaacg ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag
    16021 ctgatggcgg caataaaggg attgatatct cagctccacg gggtacacct atttacgccg
    16081 cgggtgcagg aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca
    16141 tgattaaaca cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa
    16201 ataatgggca aagcgtgaag gctgggcaaa aaatcgccac tatggggagc actgatgcgg
    16261 catcggttcg cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt
    16321 acttgccgcc tcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag
    16381 cgcggcactt gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg
    16441 tagttcaatg gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgcc
    16501 cgctccaaac aaacatctac caacgtctac taaagtctgc caatcccccg aatatcaagg
    16561 cctccagcga ttttcctcgt ctacaagcgt ctacagtcat ctacctaaat ctacacacag
    16621 aagtgtatag taatgcgtat aggacaaaat cgttttttgg gggctataca catgttgctt
    16681 tcagaccttg ctatccgccg cgcgaagcca aaagagaaag cctatacact caatgacggc
    16741 aacgggcttt cgttactcat tgagcctaac ggttcgaagg gctggcgatt gcgatacagg
    16801 tttgcaggta agcccaaaat gttgtcgctg ggtgtttatc ctgaggtgtc cctttctgat
    16861 gccagggcgg caagggatga agccaaaagg cttatcgctg gtggagttaa tccaagtgat
    16921 gtgagaaaag cccataagag agaacaggcg agcaaattcg gtaacacctt tgaggcgatc
    16981 gcccgtgagt ggtatgaaaa acgcctggac agatggtcac aatcatatgc cgcagagatg
    17041 attgatacgt ttgagaagga cgtattcccg tatatcggca gcaaaccgat agcagaaata
    17101 aaaccgatgg aattgctcgc ggttctctcc cgtcttaatg accgtggcgc aactgaaaaa
    17161 ttgcgtaagg ttcgccagcg atgtggcgaa gtgttccggt acgcaataat taccggacgc
    17221 gctgaatata accccgctcc tgatctggca agcgcatttg caccacacaa aaaagagcat
    17281 tacgcgtatc tgaaagttga tgagctaccg gaatttttcc gcgtttttaa tacgtacacc
    17341 ggaagccaga tcgtcaagct gggtatgcga ttattgattc ttacaggact ccgcccaggt
    17401 gaattgcggc aggcagagtg gcaggagatt gattttgata atcgtctgtg ggaagtgccg
    17461 aaagaacgaa tgaaaatgcg ccgtcctcat tgcgtgccgc tttcagaaca ggcgatagct
    17521 attctggaac aactgaaaca catgacgggg cagtatccgt ttattttccc tggtcgaata
    17581 cagcacagta aaccgatgag tgaaatggcg atgaacgtgc ttattcgtcg tattggttat
    17641 gcagggcgtg ttactggtca tgggttccgg cacaccatga gtacgatttt gcacgagcag
    17701 gggtataact ccgcatggat agaaacgcag ctggcgcacg tcgataaaaa ctcgatccgt
    17761 ggcacataca atcacgcgca atatctggac ggacggcggg agatgctcca gtggtacgcc
    17821 gactatatgg actcactgga acagggcgga aacgtggtac atggcaagtt tggaaaatgt
    17881 ggatgactgg ttgagtatac agtagtagac tttgagcgac taaataacag gctgtgtcta
    17941 gggtcgctcc cgaaaccccg tacacctctg cggactggca cagccaccac tacagagagt
    18001 gcgaggtgtg cacatgccgg ttgtgaatga tttccctgac ttaagtcatt ggtttacagt
    18061 gcaggagttt actattactc aagctgcgtt attgctgtca ggtattgatc ctctcgacta
    18121 cccaactctg gagatggtca aagaggaaaa acacgaacga tggagaatgg catatgggtt
    18181 tgcttatgga atggttactg caattcgacg cggtgtctta actcccgttc tgtgcgttgc
    18241 gtatgattgg ggggatatgc gtcgtttaac cccagcagag atgatggatc gttcaaataa
    18301 tatatccatg gacggaacaa ttattacccg ttattcatta caggaatggg taaaaaatga
    18361 gaaagttgat tatgtaagac cctctcgctc tatatcggat aaggagatta agccatctaa
    18421 ccaatcagta ataattgatg ccgaatctag cgtgattaat tctgaagaaa cagtattgct
    18481 gacttatggt cataagagcg aaggtcttga atatgtagaa gacgcaataa gagaactttg
    18541 ggcaacatat gatccagata atcaacaaac agcccctaca agaaatgatg ttgttgaata
    18601 cctgaaaggc aggggagcgg ggcagaacat ggccgaggct gtaaacctga tactacgccc
    18661 cactggcttg cagcaagttg gaagaaggaa aagaaaaaca aatagttaaa tagtgcccac
    18721 ccttcgtaaa aaaggtggca ctatttgctc aaaactcttt tgtttatggc gagaaatcat
    18781 agttaagcgt gcgttttctt tgcttattga tactggaatg gtggcagcat tattaaaata
    18841 atagtgggca ctattccatt gaaatagtac ccaccattaa aaatagtatc cacctataaa
    18901 tatgttttcc ttttatgcat cattgtagac ggttatagac gttacgagga ttgaatatgt
    18961 caaataactt acttcgtctt tctgatgtta tgcgccgtac tggttacggt cgcgcttgga
    19021 tttatcgcct gatcaaccag gggcgctttc ctaaacctgt aaaaattggt ccccgctccg
    19081 tggcttttat tgaaagcgaa gtcgatgaat ggattaacca gcgtatcgag gcttctcgca
    19141 acaacgccgc gtaataatct tttgggaggg ggatattctc ccccagcact aaacctgatc
    19201 cgatagcagg agtttttcaa tgaaacgtcc gaatatgccc gtacaggggc agggcttcgt
    19261 tcagcccgaa gaactgagcc aacagtattt cgccgcaatt attccggtaa tttccggcaa
    19321 tgagattatt gctgacggaa taaataaaaa tgcactcacc gcgccggaag tgtttccttc
    19381 tggcggtgag cctgtaacca cttcacaggc tatcgcccga tttttcagaa aggatcacga
    19441 taaggttgtt aaccggattg aaaacctgtg ttgttctgct gaatttttaa cccgcaattt
    19501 tcggcgggta aaatttgagc ataaggggcg cgtattgaaa gcctacgaag tcaccaaaga
    19561 cggcttcgtg tttttaacaa tgggatttac cggaaaaaaa gcggctgcat tcaaagaggc
    19621 atacattgcg cagtttaacc gcatggctaa ctggatagca gagagatgtg aggccaaagc
    19681 tgagatgcgc tctatggggg atgccatgaa gggatacatt gagcgcactg gagatactca
    19741 acacggtcat gcttataaca acgaatgtac tttcattgcg cgccttgtat tagggatgca
    19801 cccgcctgta tgggccaaaa aaaacggcat cccatcaaaa aaagttcgtg accatatgac
    19861 cgctgatcaa ctggcgttgc tcgcgtatct ggaaagccgt aactgtgcgc tactggatat
    19921 ggatatgcca acgccagaac gtaaagaacg tctcaccgaa ttagcccaac gctggctggt
    19981 ccgtcgcctg ggagaacgtg aacatgcctg acatcctcaa acctgctgtc cggtacatcg
    20041 acacggcgga taagttcgca ctactgaaca tcaaccatgc ggattacatg cgccatatcg
    20101 tgagccattt caccgctgag cgcatgaaac acgttaagca atacttggag gagggtttat
    20161 caaattatcg cgctggtggc cctgtacggc gcgaatatcc gcatgatgca ggaatggcag
    20221 gcaaaacaaa ggcgaccacg aaagggtcgc cagtggagat aaaggaaatc aagcgcatta
    20281 tccacaatgc catatctgag gctggcggac aatatgcagt ttacgacgaa tggccccttg
    20341 gtcccgatgt tcgtattttc ttacggcatg tacttttcag aaaattatca agtgcaaaag
    20401 cttcaggtgc ttctctgtgt tcccgtgcca gagcgttaag ttctttacgt gttaattctg
    20461 ttgctcttgc ctgttcgcgt gcagctttcc gctttcgttc atgttcagtt atacgggcgc
    20521 tggtcagtgt aaccatcatg gcaaaggagt tttttttctg gtcatccata agctatccct
    20581 tattgcttga tcggctggaa tgctgcttta aaaaatcctc aagcgcaaaa ttggtattga
    20641 aagttttttc aaatcgcttg cgcatatcca tatctgcgat gtgtctcgtt attgactgtt
    20701 ttttttcaac gaaatgttgc tcatatgctg tgagatcatc ttttttattc tctgattgtg
    20761 aagtgtgttg tgtgttatcc atattgtgca cctgtaaaac aatgcgccgt acttcctcac
    20821 atcacggcgc tgatggtttc tgtctgtttc atagagccgc agaagaaaca gcgagctatc
    20881 agcctggcgg tcggttttta gtggctttta accgccgtgg gtatttctcg acaaaagcct
    20941 caagggcaaa tcgtgttggc ctggtgagtg gctgtggatt aagtaacaac cttctttgcc
    21001 gttgtgcttt ttcgcgctgt cgctcaagta tggataatgc gatttcctgt agttcttcat
    21061 ttgtcattgt ctgtacctgt aaagcaatac gccgtaacac accacgctac agcgatgatg
    21121 gtttctgttg ttgtaattta ttgcgttggt tttttctgcc actgagaaac acctcaagcg
    21181 gtggcggttc gctggttttt tctggcgggg ttaatttttc tttcctggtt tcaatgatgt
    21241 cacgtatcag tcgctggcgt tgtagtgcgc tgatattcgg ggtaacgggc gaaaatctgt
    21301 gcttactcat actggattcc ggtaatgcat tatgtcgtga tgcctcatat cacggcgctg
    21361 gttattgtct ggtacgttgt ctttctttct ggtatccatc ctttctggaa ggatatgtga
    21421 atatttttcg cacaaacttt tctcgcaaca catccgacca cttaagcaaa aaagcggcat
    21481 atactgaggc tatcccttat aaaacgggga tcgggattgg cctcccgctg gagttagagg
    21541 cgatacacga cgcgccagcg tctttttttg tatcggcgca cacgcacacc tcaacaatgg
    21601 tgggctgtat ggaacttcct tcgggaaggc tggtttcctc taacaccagt aaggccaatt
    21661 ccgtacagtc taccgccagc aagattggcc tcttgggcgg tgggtataat cccaaagtca
    21721 gaggagcctt tcacatggct gtatccgcac gcccttactt tgtctggcgc tttatgcagt
    21781 gcctgacaga cagcattgca atattcaccg ttaccgctgc cactgagcgc gaagcacgcg
    21841 cccagctgcc gcacgcacat cttatttttg tcgcccgtat ccgtcaggag gtgactcatg
    21901 cttaaaacct tccgcgtctt ttcccgtgct gttaacaagc gtggctatac cgttggcatc
    21961 acgcagaacg tgaaggccga aaacgtccag gctgcaatca gcacagtaat taacaaatca
    22021 gctaaagccg gtttatccgg cgtaattatc cacgcagtga catgtattca ggatgaggag
    22081 taattcatgt gtaatttttc cgatatgact gttgatgaag cagcaaaaag cgcaatgcag
    22141 gcagaactta tttgttctct gatgatgggt aacacaggtg aaatgactga gggtgagatt
    22201 gagtcattgc tcgccctgat taagcagctt acaggtaagg ctggtagctg gctgattgcg
    22261 gcatcgggtg atatgcaata agggaataaa taacaggatg aaataaatac cagcgaatta
    22321 aagcgttaca tatcaggaaa taaaacgatg aaacgtaaat attctggctt aactgccagt
    22381 agcaaaactc accccgaatt tatgtgcggg gatacttatc gcaatgaata cggtagccac
    22441 atgacgatga tcagcagagg taagaaatga aaagcgcacc gaacttaaaa aaacagcctc
    22501 gagggaaaaa acacgccgat acggaagtga ttattttcgc tggcagtgat gcgtgggcgc
    22561 acgcgaaaca gtggctggag caggacggaa aacttgccgg agataatata ccgcccgtcg
    22621 tgctggctga tgagcaactt aaagatattg gcaatctgca aattgtaccc gacgggcgca
    22681 aatcagcgcg tatatacaag gccggacacc tcgatcaggt gatggtcaaa ggcataggtc
    22741 agaagctggc ggcggcaggc gtacaggacg cagactatta ccccgaaggt atgcaccata
    22801 gcgagcggca aaactggcgt aactatctgg atattgaacg caaaaacatt tctgacgggc
    22861 tggttattga gttgcctgta aagaaaaaag cgcgtggaca gggtgatgat tcgtcactgg
    22921 cacttaacca gatgggggca agccagcgcg gagaagttct tctggcacat tatggcggcg
    22981 aactggcaat aaacgccgac tctgacaccg tgcattatta caacagtgtt gtatgggttc
    23041 ccgtacagga taaagagcta cagcgcacaa tggcgcaaat cttcattgac gaagatatct
    23101 gctattcaca aaacgccata aaatcggcgg ttgataccat gaagttaagt ttacccgtaa
    23161 tggggaatac cgcccgtaac ctgattggat tcagtaacgg ggtatttgat acccgtacag
    23221 gtgatttccg tgaacacgat aaaaatgact ggctgttaat tgccagtgaa ttaccgttca
    23281 ccccaccagc agagggtgaa acgctggcaa cacatgcgcc gaatttctgg aagtggttac
    23341 gccgttcggt ggctgagaat gaccgcaagg cagatcgcgt tctggcagcg ttatttatgg
    23401 tgctggcgaa ccggtacgac tggcagttat tcattgaggt aacaggtcct ggtggaagcg
    23461 gtaaaagtgt tatggcagag atttgtacca tgctggcagg taaggccaac acggtatcgg
    23521 caagcatgaa ggcactggag gatgcaaggg aacgcgcatt agtggttggc ttttcactga
    23581 ttattctgcc ggatatgacc cgctacgcgg gtgatggtgc agggataaag gccattacgg
    23641 gcggtgacaa ggtggcaatt gacccgaaac acaaagcgcc atactcaacg cgtattccgg
    23701 cggttgtgct ggcggttaac aacagcgcca tgtcattcag tgaccgcagc ggggggatct
    23761 cacgtcgtcg tgtgatattc aatttttcgg aggttgtacc ggaaaacgaa cgcgatccga
    23821 tgctgccgga aaaaatagaa ggtgagcttg ctgtagtgat tcgccatttg cttacgcgct
    23881 tttctgacca ggacgaagcc agactcctgt tacatgagca gcagaaatca gaggaagccc
    23941 ttgcgataaa gcgtgagggg gattcgctgg tggacttctg cggctatctc atgtcgtcgg
    24001 taatgtgtga cggcctgtta gtcggtaatg ctgagattat tcctttcagt ccacgccgtt
    24061 atctctatca cgcctatctg gcttatatga gggcgcacgg gttcagtaag cccgtaacac
    24121 tgacgcgctt tggtgcggat atgccaggtg caatggcgga atatggcagg gaatatatga
    24181 aacggaaaac gaagcacgga ttgcgctcaa atgtgacgct gacagaggaa tcggaggact
    24241 ggatgccgtc atgtgcacca gtgaaaaatg agggtgataa aaattaagtt atagaaaaac
    24301 tgttcaccac tgttcaccct gtaataaatg tatttagtat caatacgtta aggggtgaac
    24361 agttttgtaa aactattcac ccaagtgttc acctgttcac catgttgttt gatttttgaa
    24421 cagcaaaggg tgaacagggt gaacagttgg tgaacacctt tacaaaaagt gttcacccct
    24481 taagttagtg aaaataaagg caaaacagca aaaggtgaac agttgaacac cttttgggta
    24541 taaattttta tttattcctc tgagaaaaat tttatgacag agaaacacgc caaaaaatca
    24601 cattcacacg ctatcgattt aaccgaaccg gaatctgttg tgaggttcgt atcggtacga
    24661 ggcagagcca cgctttatat acctgacgaa catctgcatc actgcgacaa acataaactt
    24721 ccgatcctga ttgtctggaa gcgaacggta tacgccgatg tgacatggct gaatgattcg
    24781 ctgatgttga tacaccgtga cctgtttgaa cgtgaagagt tccggcggga tattgaagag
    24841 cgggcaggaa aaatctatga gaaatatgca gcaaacagca aaagagccgc acgggcgatc
    24901 acttaccatt tcatgacgct gtatgacctg aaagcggatg atgcagaaaa agcggcatgt
    24961 gatttattcg atatgacaat gggtattatc caggagtacc ggaacaagga gcgcagacca
    25021 tgacagcaca gattgcagct tacgggcggc tggtggacga cccgcaggta aaacagacca
    25081 gcaagggaac agcgatgacg ctggcgcgta tggcggtatc tttgccatgc agtcaggcac
    25141 aggacggaca ggcgacgtta tggctatcag tcctggcatt tggtaagcag gccgacgcgc
    25201 tggcgagaca tgaaaaaggc gaccttatca gcgtggcggg taacatgcag attagccagt
    25261 ggaccggaca gaacggagaa atacggcagg gctggcaggt tatcgctgac agcgtaatca
    25321 gtgcccgttc ggcgcgtcct ggcgggaagc gggggcaaca ggggaaggcg accgaagcac
    25381 tggaaagagc aaaacgacag accggacagc agggaaatca gccaccagcg ggaggtgatg
    25441 acccgttctc tgacgatatt ccgttctgaa tgacaggagc acaacatgaa ccagcagacc
    25501 acacaacagg accagaccac acgtaaacca tcacggcgca taccgtggca gcacatgaca
    25561 aaagtgcaac gaaaaaacca cagacgaata aatcgaattg cggaatactg gcctgagctg
    25621 tttgggacag agaatataaa acccctgaag gctggcataa tacgtgatgt gcttgaggac
    25681 gctaaatcac gggagttgcc acttgctaca ggtgtattac gcggcgcatt aacctcacac
    25741 cttcacacga cgcgatacct taaggcgatt attgctggtg gcccacggta tgacctgaac
    25801 gggcagcctt gcggcgaagt cacggaagaa gataaatcag tcgccagtga actactgaaa
    25861 cggagactgg cgcaaattaa gcagcagaac agccagcagc aaacaaccgg agacgatggt
    25921 gatgatagct gacagcgtgg cgcaaaaact tgaagaacgc ggattatggc gacgagcagc
    25981 tacccgatgg ggtgacgtcc ttcttcacgc agaaacggac agggaacgcg aggaagctgc
    26041 cagacgtcgg gcgatatgta tcattaaatc ccgtcgtatg cctgaacaac tggtcacctt
    26101 tggcgatgtg aaaaaggccg ctgacagaac gctgaaagag atggggatta atgcgcagga
    26161 tgaatggaaa aattacagct tcagtgatgc aggcgacgat ttagcgctac cgtgatacgt
    26221 atctcatcac cgtatttgct cagttatcgc gattcgctat aattaccacc gaagagccgt
    26281 tgatggtgtt cgcctctggc aactggagcc agactgcatg aagccattca gcggcttttc
    26341 ggcactcatt ttgatgtgta cttgtcacac acatcgggat gagtgaacga cgaaaacatc
    26401 acggtgattt cgtcatgccg cgataagaat gcgcctcttt gggcaactgg agccctctgt
    26461 acagcgggtt attgcggcaa agttgtgata aaaacgcgcc tctttgagca attgaagctc
    26521 actgtaaagc ggcttatcac agcaggctgt aacgggcgct catcttctgg caactgaagc
    26581 cacctgtaca gagacccgtt acggctgacc ctgaaggatt tcgccccgtg caattgaagc
    26641 acccagcgga gttattcagg gttttttatt gcccgtccac cagcacaacc agcaccctta
    26701 ccagtacggt taacctggtc attatcaggc aggccagaag ccccttcggt ggggtgaaaa
    26761 atccacggaa aaatccctga aaaaatgcac agaaaattgc gcaaaaaaat gcgcaaatcg
    26821 atcacaaaac cgatcacaaa accgatcaca cagaccagca gagcaaagcc agatatgacg
    26881 aggcttgcag ggggacagac cacctcaaaa agtgcgcaaa aaactgcgca tttttctcaa
    26941 aaacgaaacg caacagccgc gcaaaacgaa accgatacgt gatgatcgaa aaagtgcgca
    27001 aatttaccgg attcaggcaa ggctgtttac cggattcggg taagcaattt tgtctgattt
    27061 gtgcaaaata tggtgttgcc gtaatagtga cattgtcatc aaaacaggga cacacaggag
    27121 ggccgatgaa tgagcgttca ccaacagagg taattcacgg gattattgaa gaacaggagg
    27181 agcgcgattt aatcctcatg agtgaagagc agacagaacg ccagcgcaaa caggaggcca
    27241 cacagccgga cagcgtgacc tctgaaaatt atgcgcgtgt tgtggctgat gtgccgtgat
    27301 ggtgtcgaac tgagtagctt taagcgactc tgtgactagt tttaaacaag tcgcaaaaag
    27361 agccggagaa aaactacgaa tctggggttt ttgctaagca ctctgtacac ggtggaacat
    27421 atggtggtca ggagacggca ggagcaacgc cagaacgcgc ccccgtacca gtggcataag
    27481 ccggagcggt tacagcggta acttgttgat taaaaggcgg ctcaattctg agccgggtag
    27541 ctgacggcgt taactggctg atattgtacc aaagcacttt ttaatgcttt ggtgctgtaa
    27601 cttactgaac ttaaagcaaa gcgcaaaatt gcgctggcta caatgaaaaa cgtaatcgcc
    27661 tgataattat gccacgagac aaagggcggt gcaccctgaa tggtcataat gaccactcag
    27721 accatcaagg caactataac gaacatcgtt acagttggag cttgtacccc gttgggggaa
    27781 aaggtgcata cctgaatcat cattatggtg atcaagggta tgtaaagccc accagcctga
    27841 ttaaaggtta accggaaaaa gccaggtatg gagggagtgg ttagccacga acaagccgga
    27901 caccaccagc agaaggtatt atgattttcg taataccttt agcaaagcgt gaatcatcat
    27961 taccgtaggg atggtcataa tgaccacacc ttcccccact ggaacaagga aggctggctt
    28021 acgaaaattt cgtaaccctc cccagcacaa tgtattaaat aatcactaca ggccattgat
    28081 taaacttaat tatcgcgaat cgcgagaatt agccccgccg gaacaaggaa agggaaatta
    28141 cgaaaatttc gtaaaatccc cccacatctc ccacggttgc ccgttaagtg caagcgcaca
    28201 ggcacgacct cattaaccag ttaccagcgc tcacgctcaa acatccacag caatggggct
    28261 aaatcgcatt ctggcgcaat gttcactgtc agggcgagca ttcaccggaa aacgtcatac
    28321 aggcttacag gcggcttaaa attcgatggg cggaggaccg gatgggaatc gggagacctg
    28381 gacaaaaagt gcaggcctcc atgacacggt caggacagtt tgtgtttaac cagaatattt
    28441 agcgcctgta ttggggcttc gtggagctcc cgcagacatt cagggtcagt aacatgttcc
    28501 tggtatcttg ttttctcaag taacaagcgg tgcatatgca gcatacgggc aagagccgcg
    28561 cacacctcct caaatgcggt atcccgctta ccgtcactgt cagagccaca aaacagcggt
    28621 acaacctcca cgccgtcgct gtgggccttt agccaggcat caaccacggt taaatcatcg
    28681 gtgaagaacc acgcactgtt agcgctgaat cgcattttcc aggcttccgg tactggttgc
    28741 ggggagtatg aatatgctgg tggtatcttg tcgtgagtat ccataggcta tcgttccgtc
    28801 attctgttat tcaccacagc gctaaaaacg ccatgaagga ccagaatacc gattgcctcg
    28861 tttgctgtat gaggtgctta ccacacattg ggtacacaga tgaatcaggc ggaagtgtga
    28921 ttaccggaaa aaaccccttt cagtttgacg aaaaaaaaac cgcagtgtga taacactacg
    28981 gcacgccacc aaagaaacat aacaaggaat cagcaatgag taatacctgt aagccttcgg
    29041 ggtgattgta acacatgagc aatcttttac tggaagctgt ttttcactgg atcacgtatc
    29101 gttatgggga ttcccatatc ggagagcatc caccagcttc atagcggata accggaaaaa
    29161 ttccggtaaa tatttgcctg agtaagtcat caagcgcctg ctaaatgtgt atagaaatgt
    29221 gtataggatt gattttagaa tatgtgggaa tggctttatt gctggtggca tggcttgttg
    29281 ttctgttccc ttcgccgctc caatttatct tcacatcatc tacctgccat ttttgttgta
    29341 tctgattttc tatattatct taattcaagc gcttcagact tttcgcaatc gccgtaatac
    29401 ttccccattt ttttgtaaat aaaaagcgtt tacgcaatgc aatcgtcatg tat
//
LOCUS       DAZGAP010000041        29353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGAP010000041 DAZGAP010000000
VERSION     DAZGAP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.041"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..2173)
                     /gene="glyS"
                     /locus_tag="ACHLAM_001535"
     CDS             complement(104..2173)
                     /gene="glyS"
                     /locus_tag="ACHLAM_001535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO7853087.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2183..3094)
                     /gene="glyQ"
                     /locus_tag="ACHLAM_001536"
     CDS             complement(2183..3094)
                     /gene="glyQ"
                     /locus_tag="ACHLAM_001536"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO7853088.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3189..3497)
                     /locus_tag="ACHLAM_001537"
     CDS             complement(3189..3497)
                     /locus_tag="ACHLAM_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO7853089.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            3671..4666
                     /gene="wecH"
                     /locus_tag="ACHLAM_001538"
     CDS             3671..4666
                     /gene="wecH"
                     /locus_tag="ACHLAM_001538"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO7853090.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4708..5145)
                     /gene="yiaA"
                     /locus_tag="ACHLAM_001539"
     CDS             complement(4708..5145)
                     /gene="yiaA"
                     /locus_tag="ACHLAM_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO7853091.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5190..5531)
                     /gene="yiaB"
                     /locus_tag="ACHLAM_001540"
     CDS             complement(5190..5531)
                     /gene="yiaB"
                     /locus_tag="ACHLAM_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO7853092.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGILMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(5700..7154)
                     /gene="xylB"
                     /locus_tag="ACHLAM_001541"
     CDS             complement(5700..7154)
                     /gene="xylB"
                     /locus_tag="ACHLAM_001541"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO7853093.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWSMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7226..8548)
                     /gene="xylA"
                     /locus_tag="ACHLAM_001542"
     CDS             complement(7226..8548)
                     /gene="xylA"
                     /locus_tag="ACHLAM_001542"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO7853094.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8914..9906
                     /gene="xylF"
                     /locus_tag="ACHLAM_001543"
     CDS             8914..9906
                     /gene="xylF"
                     /locus_tag="ACHLAM_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7853095.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9984..11525
                     /gene="xylG"
                     /locus_tag="ACHLAM_001544"
     CDS             9984..11525
                     /gene="xylG"
                     /locus_tag="ACHLAM_001544"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO7853096.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11503..12684
                     /gene="xylH"
                     /locus_tag="ACHLAM_001545"
     CDS             11503..12684
                     /gene="xylH"
                     /locus_tag="ACHLAM_001545"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO7853097.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12762..13940
                     /gene="xylR"
                     /locus_tag="ACHLAM_001546"
     CDS             12762..13940
                     /gene="xylR"
                     /locus_tag="ACHLAM_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO7853098.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14048..14872)
                     /locus_tag="ACHLAM_001547"
     CDS             complement(14048..14872)
                     /locus_tag="ACHLAM_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO7853099.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15192..17219
                     /gene="malS"
                     /locus_tag="ACHLAM_001548"
     CDS             15192..17219
                     /gene="malS"
                     /locus_tag="ACHLAM_001548"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7853100.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKYKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALVEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAA
                     SVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17397..18650
                     /gene="avtA"
                     /locus_tag="ACHLAM_001549"
     CDS             17397..18650
                     /gene="avtA"
                     /locus_tag="ACHLAM_001549"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO7853101.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMSEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18802..19275)
                     /locus_tag="ACHLAM_001550"
     CDS             complement(18802..19275)
                     /locus_tag="ACHLAM_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7853102.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19377..20225)
                     /gene="yiaJ"
                     /locus_tag="ACHLAM_001551"
     CDS             complement(19377..20225)
                     /gene="yiaJ"
                     /locus_tag="ACHLAM_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO7853103.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20426..21424
                     /gene="yiaK"
                     /locus_tag="ACHLAM_001552"
     CDS             20426..21424
                     /gene="yiaK"
                     /locus_tag="ACHLAM_001552"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO7853104.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21436..21903
                     /locus_tag="ACHLAM_001553"
     CDS             21436..21903
                     /locus_tag="ACHLAM_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO7853105.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
     gene            22021..22494
                     /gene="yiaM"
                     /locus_tag="ACHLAM_001554"
     CDS             22021..22494
                     /gene="yiaM"
                     /locus_tag="ACHLAM_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO7853106.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22497..23774
                     /gene="yiaN"
                     /locus_tag="ACHLAM_001555"
     CDS             22497..23774
                     /gene="yiaN"
                     /locus_tag="ACHLAM_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO7853107.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23787..24773
                     /gene="yiaO"
                     /locus_tag="ACHLAM_001556"
     CDS             23787..24773
                     /gene="yiaO"
                     /locus_tag="ACHLAM_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO7853108.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            24777..26273
                     /gene="lyxK"
                     /locus_tag="ACHLAM_001557"
     CDS             24777..26273
                     /gene="lyxK"
                     /locus_tag="ACHLAM_001557"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO7853109.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            26270..26932
                     /gene="ulaD"
                     /locus_tag="ACHLAM_001558"
     CDS             26270..26932
                     /gene="ulaD"
                     /locus_tag="ACHLAM_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7853110.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            26925..27785
                     /locus_tag="ACHLAM_001559"
     CDS             26925..27785
                     /locus_tag="ACHLAM_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO7853111.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27779..28474
                     /gene="araD"
                     /locus_tag="ACHLAM_001560"
     CDS             27779..28474
                     /gene="araD"
                     /locus_tag="ACHLAM_001560"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7853112.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28506..29321)
                     /locus_tag="ACHLAM_001561"
     CDS             complement(28506..29321)
                     /locus_tag="ACHLAM_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7853113.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
BASE COUNT         7205 a         7541 c         7388 g         7219 t
ORIGIN      
        1 atatatgaga caatctttat tcagcagaag attattaaag gttgctctat tatttagcga
       61 taaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgt aacagcgaaa
      121 tatccgcaac gcgcaggaat aattcgcgca gtttctccag catggtaaga cggttgattc
      181 gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaggcatca accggttcac
      241 gcaattcagc cagttcgacc agcgcgtcct ggtaacgacc ttcagcgaag tacggctcca
      301 gcttgtcgcg cagcaccaca acttgcatcg ccagtttaat ttcttccggc tctttcagag
      361 tagaagcatt cacgcgatcg ctcagcactt cgtcagactt cgccagaatg ttagatacac
      421 gcttgtttgc cgccgccagt gacgctgccg catccagagt acggaagtgc gataccgcct
      481 tcatacgggc atcgaaatct gccggacgag tcggacgacg cgccagtacc gcctggatgg
      541 tatcaacggt gtaaccttcg tcctgatacc aggcgcggaa gcgaccgagc ataaagtcga
      601 taacatcatc aactacgttg gtattagtca gcttatcgcc atacagacgc accgcttctt
      661 cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataatacgc agcacgccaa
      721 gtgcggcacg acgcaacgca aacgggtctt tgtcgccttt cggatgctgg ccgataccga
      781 agatacccgc cagagtatcc atcttgtcag caatcgccag cgcacaggct accgggttag
      841 acggcaggtc atcaccggca aagcgcggct gatactgctc gttcagcgcc acggcaacat
      901 cttccgcttc gccatcgtga cgcgcgtagt gcatccccat aacgccctgg gtgtcggtga
      961 actcgaagac catgttggtc atcaggtcgc acttggacag caggcccgca cgggtagcgt
     1021 ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg atgcggtcag
     1081 tcttgtcacg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg cgcggcaggt
     1141 tatcttccag acgtttttta cggtcggtgt tgaagaagaa ctcggcatcc gccagacgtg
     1201 gacgaacgac tttctcgtta ccagagataa tttgctgcgg atctttcgat tcgatgttgg
     1261 caacgaagat aaagttcggc agcagtttgc cgtcgttcgc atacaccggg aagtatttct
     1321 ggtcaccttt catggtgtaa accagcgctt cagacggcac cgcgaggaat ttctcttcga
     1381 attttgcggt cagcacaacc ggccattcca ccagcgaagc gacttcttcc aacaggcttt
     1441 cgcttaagtc agcgttaccg ccaatcttac gcgccgcttc ttcggcatcg gctttaatct
     1501 tcgccttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga atttccggat
     1561 actgatcagc attgtcgata gtgaattccg gctcgcccat aaagcggtgg ccgcgaatca
     1621 cgcgatcgga ctgaatgccc agaatggtag ccggaataac tttgtcgccc agcagcaggg
     1681 tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc accccaacgc atcagtttcg
     1741 ggatcggcag tttcgccagc gaagtcgcaa ccatattcgg cagcagtgct tcagtgcttt
     1801 cgcccttcac gtgggcgcga tacagcagcc attcgccttt atcggtagtc agacgctcag
     1861 cctggtcaac ggtaataccg caaccacgcg cccagccttc tgccgctttg ctcggtttgc
     1921 cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca cgatccggtt
     1981 gcgcttccgc caggttagct actttaagcg ccagacgacg cggagcagca aaccattgaa
     2041 cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaagttcgca gcaaaggact
     2101 cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc accagaaaag
     2161 ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag ccgagggctt
     2221 cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg cgcagaatat
     2281 agcgctgacg ctcggtgacg gagatggctt tacgcgcatc cagcaggttg aagctgtggg
     2341 cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc gccagcagct
     2401 gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag gaagtccaca tccgcgtatt
     2461 cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgcca taggtggttt
     2521 tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc tgaatgtaca
     2581 ttgccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac tccagaccac
     2641 caacctgctg gaagtaagtg aactgcgtca cttccatgcc gttcagccac acttcccagc
     2701 ccagccccca ggcacccagc gtcgggtttt cccagttatc ttccacgaaa cggatgtcgt
     2761 ggatggtcgg gtccatgccc agctctttca gagaaccaag gtacagctcc tgaatattgt
     2821 ccggcgatgg cttaatgacc acctggaact gatagtagtg ctgtaaacgg ttggggtttt
     2881 cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc gccatcggtt
     2941 ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg acttccatgt
     3001 ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag gtcaggatca
     3061 agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga tacgtgtgga
     3121 tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga aacgggaaaa
     3181 agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa agaacagaca
     3241 aactgctctt tacggaaggt ataaccgcgc atttcgtagc ttccctggaa ctgttcaaat
     3301 gcggtgacgt cgattttttg ctcgccggtg ttataacgtt tcgccgcctg atccttgcat
     3361 aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc tttctgcacg
     3421 ggtggcggtg tagaacaacc cactagcgcc ataagcacca taagcgccat tgccggaaag
     3481 aaagagttca tcatcattaa attaccgtct ggtttgcagg tgagtcttat tatttatatc
     3541 ggtatagtgg ctgatatctt acgaatttcc ttaagcctct ataagctgta gtgataaaac
     3601 tcagattatt ccagtgcgcc aaccgaatga tttatcggtc agactttttt aaccagatac
     3661 agagacacag atgcagccca aaatttactg gattgataac ctgcgaggga tagcgtgttt
     3721 aatggtggtg atgattcaca ccactacctg gtatgtgacc aatgctcata gtgttagccc
     3781 cgtcacctgg gatatcgcca atgttctgaa ttctgcctct cgtgtcagcg tgccgctatt
     3841 tttcatgatt tccggctatc tcttttttgg cgaacgcagc gcccagccgc gccatttctt
     3901 gcgtatcggc ttatgtctgc ttttttatag cgcaatcgca ctgctctaca ttgcgctgtt
     3961 tacctccatc aatgtggagt tagcgctgaa aaacctgctg caaaagccag tgttttacca
     4021 cttatggttt ttcttcgcga ttgcggtgat ttatctggtt tcaccgctga ttcaggtgaa
     4081 gaacgtcggc ggaaaaatgt tgctggtgct aatggtggtg attggtatta tcgctaaccc
     4141 aaacacagtg ccgcagaaaa ttgacggttt tgaatggctg ccaattaact tatatatcaa
     4201 tggcgatact ttttactaca tcctgtatgg catgttgggc cgcgctatag ggatgatgga
     4261 cacacagcat aaagcactgt cgtgggtgtg cgcagcactg tttgcgacgg gggtttttat
     4321 tatctctcgc gggacattat atgaattgca gtggcgcgga aattttgccg atacctggta
     4381 tctttactgt gggccgatgg tttttatctg cgcaatcacg ctattgactc tggttaaaaa
     4441 cacgcttgat acgcgtaccg ttcgcggact tggcttaatc tcccgccatt cgttgggcat
     4501 atacgggttc cacgccttga ttatccatgc actgcgcacc cgggggattg agcttaaaaa
     4561 ctggccaata ctcgatatta tttggatctt ttgcgcgacg ttggcagcga gtttgttact
     4621 ttctatgctg gtacaacgaa tcgacagaaa cagactagtg agttaagtaa aaggccggtc
     4681 acattggact gaccgggctt atgtgaatta ttcgctgtat tcttcctggg taacctgtgt
     4741 ttcttttggt gggtttattc cggcatcacg aatattcttc tgcaccgcta ctgcaccaaa
     4801 caagcttaag aagaaagcca gtccataaaa acctttctcg ctgagtaata acgttgcatt
     4861 ccacagacct accatcagta acgcaacaga gataataaag acagtcaggc aggtcatata
     4921 ataaatggag gtggtcggta tgccttcata cttgtcccga acggtctttt gataagacgc
     4981 cgcagaaaac agtcctaata ccagtacggc aaaataatat cctttttcat ttaactgcat
     5041 ctctgcattc catagcccta acagataggt aacgatacca ccaacgagag ctatccatga
     5101 cacaatactg aatgccggag aataggttga tattttgttg tccatcacag tattccttca
     5161 ttcctgaatg tgtaagagct ttatgttgct catgccgagc gaaacaaacg catttgacca
     5221 aacaagcaga caaaaaaggc gatagggtag atcaccattt cataagcatt gataggtgcg
     5281 tttaaaacac cgataaacaa gagaccgata gtgattaacg caactaactg gcagatatgg
     5341 gtaaaaaaat tatccagttg cccgagtttt tctgcgcgaa gatatgcata gttgccaaaa
     5401 gttgctgtca ttaatatgcc aagaaaatag ccttttccac ttaacaatgg gcatgagatc
     5461 catagcccaa ccagataaac cagcgccccg acaacaaata atagttttgc cacagtcttt
     5521 gaggttttca taacgatctc catatctacc agcgatacat tacgagtaac caacgaaaga
     5581 caaaactgaa aaatgccatt aacaaatgat tttcagaata aattcatact aaatattaat
     5641 taattactga gatatataga tgtgaattat cccccacccg gtcaggcaga ggataacgtt
     5701 tacgccatta atggcagaag ttgctgatag aggcgacgga acgtttctcg tcgtggctga
     5761 taagcggcat aacgctgcgc atctggtaga tgcgactgtt ctaacggtaa ctgcggcaac
     5821 aactcaatga gcgatttctc tggattcgcc gcaatctgcg ccagccttgc tgcgcccagt
     5881 gctggcccca catccccccc cgtacggtaa tcgagctgct gaccgctgat atccgccagc
     5941 atttgacgcc agtactcact acgcgccccg ccaccaatca acgtaacact ttgcggttta
     6001 atgccgcagg catgcacgac atccatgcca tctgccagcg cataacccac gccttccagc
     6061 actgctcgcg ccagttcatt ggggccatgt tgatgagtca aaccaaagaa aacccccttc
     6121 gcctggggat tattgtgtgg cgtacgctcg ccggaaagat aaggcagaaa ccaaactggc
     6181 tcggcacttt catcagcctg ttgagctgca gcgattaaag ctgggacatt gctcaggccg
     6241 gttaatttcg cggcccaatc cagacacgac gctgcactca gcattacaga cattaaatgc
     6301 caacgctgcg gtagcgcatg gcaaaagctg tgtacggcgc tttctggctt gcttaaaaac
     6361 ccttcgctga cagcaaaata gacccccgac gtccccagcg ataacattgc ctgattagca
     6421 tcaaccattc ctacaccaac cgcaccagct gcattatcac caccgcctgc gacaactggc
     6481 accgccgcca tactccacgc tttcgcaact tcaggtagca aagcaccagt aatttcgctg
     6541 ccttcgtata atgcgggcat ctggtcacga gataagtggc aagcctgcag catgtcgtca
     6601 ctccagtcac gctttgcgac atccagccac atggtgccag ctgcgtcaga catatcgctg
     6661 gcaaactccc ccgtcatacg cagacgcaag taatctttcg gtaataatac tttgtcgatt
     6721 tgacggaata tctccggctc atgccgctga acccatagca atttaggcgc agtaaatccg
     6781 ggcatcatca ggttaccggt aatcacccgt gattgcggaa ctcgcgcttc cagcaaagtg
     6841 cactcttgcg cacagcgccc gtcgttccac aaaatggcag ggcgtaatac ccgttgctgg
     6901 gcatccagta aggttgcacc gtgcatctgg ccggcaatac ccaatgcttt aacgtcctgc
     6961 agagaatgct gatcgcccag agctttcatt gcgcggtcag ttgcctgcca ccactgttcc
     7021 gggtcttgtt ccgaccagag tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa
     7081 gcaaccacct caccctgctc gttgagcaaa ataactttta cgcccgaggt gccaagatct
     7141 atcccgatat acatatcgat cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg
     7201 ggccaacgga ctgcacagtt agccgttatt tgtcgaacag ataatgattt accagatttt
     7261 ccagttgctc ctggcgacca ctctgatgca ccggagacaa attgtgttcc tgagcatatt
     7321 tggctaaatc tgccagtgac atttggcctt tcaggatttg ctggcctaat tcgctattcc
     7381 agccggaata acgctgcgcg atgcgtttat ccagctcgcc atcttcaatc atgcgtgctg
     7441 caattttcag cgccaacgcc atcgtatcca tcgcgccgat atgaccgtaa aacagatcat
     7501 atttatcagt actttgacga cgtactttgg catcgaagtt cagaccgcca gtggtgaaac
     7561 cgcctgcttt gagaatttca tacatcacca gcgcattctc ttccacactg ttcgggaact
     7621 ggtcggtgtc ccagcccagt tgtgcatcgc cacggttggc gtcgacagaa ccgaacaggc
     7681 caagcgcaat ggcggtggct atttcatgat ggaaagagtg acctgccagc gtcgcgtggt
     7741 tagcttcaat attcagttta atctcttttt ccagaccaaa ctgtttcagg aagccataga
     7801 ccgtcgcagc atcgtaatca tattgatgtt tggtcggttc ttgcggtttc ggttcgataa
     7861 gcaacgtgcc ctggaaaccg attttatgtt tatgctcaac caccatctgc ataaagcggc
     7921 ccagctgttc acgctcctgg cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac
     7981 caccccacag gacatagttt tcaccgccca atttatgggt tgcttccatc gctgtaacaa
     8041 cttgcgttgc cgcccaactg aagacttcag gatctgggtt cgtcgccgca cccgcgccat
     8101 agcgagggtt tgtaaagcag ttagcagttc cccacagcag cttcacgccg ctctcttctt
     8161 gcttgcctgc caggacatca accatttgcg caaaattatt gatgtactct tttaacgacg
     8221 cgccctcagg ggaaacatcc acatcgtgga agcaataaaa tggcacatgt aacttgtgga
     8281 aaaactcaaa tgcgacatct gctttacgct tcgccaacgc cagtgcctca ccaggctgct
     8341 gccacggacg attaaacgct cccacaccaa acatatccgc cccgttccag cagaaggtgt
     8401 gccagtagca ggcggcaaaa cgcaagtgct cttccatacg cttgcccaac accagttcgt
     8461 cgggattgta gtgacggaat gctaacgggt ttgaggattt tgagccttca taacgaacgc
     8521 gatcgagctg gtcaaaatag gcttgcatat tgaactccat aatcaggtaa tgccgcgggt
     8581 gatggatgat gtcgtaatat tgggcactcc ctttcagttg ctcaattatg ttatttcaca
     8641 ctgctattga gataattcac aagtgtgcgc tcgctcgcaa aataaaatgg gatgatgaaa
     8701 ctgggtaatt cctcgaagag aaaaatgcaa taagtacaat tgcgcaacaa aagtaagatc
     8761 tcggtcataa atcaagaaat aaaccaaaaa tagtaatcga aagataaaaa tctgtaattg
     8821 ttttcccctg tttagttgct aaaaattggt tacgtttatc gcggtgattg ttacttatta
     8881 aagctgtcct ctaactacag aaggccctac accatgaaaa taaagaacat tctactcacc
     8941 ctttgcacct cacttctgct taccaacgtt gcggcacacg ccaaagaagt caaaataggt
     9001 atggcgattg atgatctccg tcttgaacgc tggcaaaaag atcgagatat ttttgtgaaa
     9061 aaagcagaat ctctcggcgc gaaagtattt gtacagtctg caaatggcaa tgaagaaaca
     9121 caaatgtcgc agattgaaaa catgattaac cggggtgtcg atgttcttgt cattattcct
     9181 tataacggtc aggtattaag taacgttgta aaagaagcca aacaagaagg tattaaagta
     9241 ttagcttacg accgtatgat taacgatgcg gatatcgatt tttatatttc tttcgataac
     9301 gaaaaagtcg gtgaactgca ggcaaaagcc ctggtcgata ttgttccgca aggtaattac
     9361 ttcctgatgg gcggctcgcc ggtagataac aacgctaagc tgttccgcgc cggacaaatg
     9421 aaagtgttaa aaccttacgt tgattctgga aaaattaaag tcgttggtga ccaatgggtt
     9481 gatggctggt taccggaaaa cgcattgaaa attatggaaa acgcgctaac cgccaataat
     9541 aacaaaattg atgctgtagt tgcctcaaac gatgccaccg caggtggggc aattcaggca
     9601 ttaagcgcgc aaggtttatc agggaaagta gcaatttccg gccaggatgc ggatctcgca
     9661 ggtattaaac gtattgctgc cggtacgcaa actatgacgg tgtataaacc tattacgttg
     9721 ttggcaaata ctgccgcaga aattgccgtt gagttgggca atggtcagga accaaaagca
     9781 gataccacac tgaataatgg cctgaaagat gttccctccc gcctgctgac accgatcgat
     9841 gtgaataaaa acaacatcaa agacacggta gttaaagacg gattccacaa agagagcgag
     9901 ctgtaagcgt tacgccccgg cgaggagcgg gggcgtgatt tctctccatg ccgtgtgaat
     9961 taattggctt aggtggagtc gttatgcctt atctacttga aatgaagaac attaccaaaa
    10021 ccttcggcag tgtgaaggcg attgataacg tcagcttgcg gttgaatgct ggcgaaatcg
    10081 tctcactctg tggggaaaat gggtctggta aatcaacgct gatgaaagtg ctgtgtggta
    10141 tttatcccca tggttcctac gaaggcgaaa ttatttttgc gggagaagag attcaggcga
    10201 gtcacatccg cgataccgaa cgcaaaggta tcgccatcat tcaccaggaa ttggccctgg
    10261 tgaaagaatt gaccgtgctg gaaaatatct tcctgggtaa cgaaataacc cataatggca
    10321 ttatggatta tgatctgatg acgctacgct gtcagaagct gctcgcacag gtcagtttat
    10381 ccatttcacc tgatacccgc gttggcgatt taggacttgg gcaacaacaa ctggttgaaa
    10441 ttgccaaggc acttaataaa caggtgcgtt tgttaattct cgatgagccg acagcctcat
    10501 taactgagca ggaaacgtcg gttttactgg atattattcg cgatctacaa cagcacggta
    10561 tcgcctgtat ttatatttcg cacaaactca acgaagtcaa agcgatttcc gatacgattt
    10621 gcgttattcg cgacggtcag cacattggca cgcgtgatgc tgctggaatg agtgaagacg
    10681 atattatcac catgatggtc ggacgagagt taaccgcgct ttaccctaat gaaccacata
    10741 ccaccggaga tgaaatatta cgtattgaac atctgacggc atggcatccg gttaatcgtc
    10801 atattaaacg agttaatgat gtctcgtttt ccctgaaacg tggcgaaata ctgggtatcg
    10861 ccggactcgt tggtgccgga cgtaccgaga ctattcagtg cctgtttggt gtgtggcccg
    10921 gacaatggga agggaaaatt tatattgatg gcaaacaggt agatattcgt aactgtcagc
    10981 aagccatcgc ccaggggatt gcgatggtac ccgaagacag aaagcgcgac ggcatcgttc
    11041 cggtaatggc ggttggtaaa aatattaccc tcgccgcact caaaaaattt accggtggca
    11101 ttagccagct tgatgacgcg gcagagcaaa aatgtattct ggaatcaatc cagcaactca
    11161 aagttaaaac gtcgtccccc gaccttgcta ttggacgttt gagcggcggc aatcagcaaa
    11221 aagcgatcct cgctcgctgt ctgttactta acccgcgcat tctcattctt gatgaaccca
    11281 ccaggggtat cgatattggc gcgaaatacg agatctacaa attaattaac caactcgtcc
    11341 agcagggtat tgccgttatt gtcatctctt ccgaattacc tgaagtgctc ggccttagcg
    11401 atcgtgtact ggtgatgcat gaagggaaac taaaagccaa cctgataaat cataacctga
    11461 ctcaggagca ggtgatggaa gccgcattga ggagcgaaca tcatgtcgaa aagcaatccg
    11521 tctgaagtga aattagccgt accgacatcc ggtggctttt ccgggctgaa atcactgaat
    11581 ttgcaggtct tcgtgatgat tgcagctatc atcgcaatca tgctgttctt tacctggacc
    11641 accgatggtg cctacttaag cgcccgtaac gtctccaacc tgttacgcca aaccgcgatt
    11701 accggcatcc tcgcggtagg aatggtgttc gtcataattt ctgctgaaat cgacctttcc
    11761 gtcggctcaa tgatggggct gttaggtggc gtcgcggcga tttgtgacgt ctggctaggc
    11821 tggcctttgc cacttaccat cattgtaacg ctggttctgg gattgcttct cggtgcctgg
    11881 aacggatggt gggtcgcgta ccgcaaagtc ccttcattta ttgtcaccct cgcgggcatg
    11941 ttggcatttc gcggcatact cattggcatc accaacggca cgaccgtatc ccccaccagc
    12001 gccgcgatgt cacaaattgg gcaaagctat ctacctgcca gcactggctt catcattggc
    12061 gcgcttggct taatggcttt tgttggctgg caatggcgcg gaagaatgcg ccgtcaggct
    12121 ttgggtttgc aatctcccgc ctctaccgca gtagttggtc gccaggcttt aaccgctatc
    12181 atcgtattag gcgcaatctg gctgttgaat gattaccgtg gcgttcccac tcctgttctg
    12241 ctgctgacgt tgctgttact cggcggaatg tttatggcaa cgcggacagc atttggacga
    12301 cgcatttatg ccatcggcgg caatctggaa gcggcacgtc tctccgggat taacgttgaa
    12361 cgcaccaaac ttgccgtgtt cgcgattaac ggattaatgg tagccatcgc cggattaatc
    12421 ctcagttctc gacttggcgc tggttcacct tctgcgggga atatcgccga gctggacgca
    12481 attgcagcat gtgtgattgg cggcaccagt ctggcaggcg gcgtcgggag cgtggctgga
    12541 gcggtaatgg gggcattcat catggcttca ctggataacg gcatgagtat gatggatgta
    12601 ccgaccttct ggcagtatat cgttaaaggt gcgattctgt tgctggctgt atggatggac
    12661 tccgcaacca aacgccgttc ttgattttga taaaaatttt ctcaaagccg gtaacgtatt
    12721 accggttttg agtttttgca tgattcagca ggaaaagaac catgtttact aaacgtcacc
    12781 gcatcacatt actgttcaat gccaataaag cctatgaccg gcaggtagta gaaggcgtag
    12841 gggaatattt acaggcgtca caatcggaat gggatatttt cattgaagaa gatttccgcg
    12901 cccgcattga taaaatcaag gactggttag gagatggcgt cattgccgac ttcgacgaca
    12961 aacaaatcga gcaagcgctg gctgatgtcg acgtccccat tgttggggtt ggtggctcgt
    13021 atcaccttgc cgaaagttac ccacccgttc attacattgc caccgataac tatgcgctgg
    13081 ttgaaagcgc atttttgcat ttaaaagaga aaggcgttaa ccgctttgct ttttatggtc
    13141 ttccggaatc aagcggtaaa cgttgggcca ctgaacgcga atatgcattt cgtcagcttg
    13201 tcgccgaaga aaagtatcgc ggagtggttt atcaggggtt agaaaccgcg ccagagaact
    13261 ggcaacacgc gcaaaatcgg ctggcagact ggctacaaac gctgccaccg caaaccggga
    13321 ttattgccgt tactgacgcc cgggcgcggc atattctgca agtatgtgaa catctacata
    13381 ttcccgtacc ggaaaaatta tgcgtgattg gcatcgataa cgaagaactg acccgctatc
    13441 tgtcgcgtgt cgccctttct tcggtcgctc agggcgcgcg gcaaatgggc tatcaggcgg
    13501 caaaactgtt gcatcgatta ttagataaag aagaaatgcc gctacagcgg attttggtcc
    13561 cgccagttcg cgtcattgaa cggcgctcaa cagattaccg ctcgctgacc gatcccgccg
    13621 ttattcaggc catgcattac attcgtaatc acgcctgtaa agggattaaa gtggatcagg
    13681 tactggatgc ggtcgggatc tcgcgctcca atcttgagaa gcgttttaaa gaagaggtgg
    13741 gtgaaaccat ccatgccatg attcatgccg agaagctgga gaaagcgcgc agtctgctga
    13801 tttcaaccac cttgtcgatc aatgagatat cgcaaatgtg cggttatcca tcgctgcaat
    13861 atttctactc tgtttttaaa aaagcatatg acacgacgcc aaaagagtat cgcgatgtaa
    13921 atagcgaggt catgttgtag cgatgatgag aattgtcggc gtcgcatcag gtaactcagc
    13981 acaaccacct gatgcaagca aaagccttat ccgactgaca agtcggataa ggctcgagga
    14041 aatgcaatta catatgcgcc gcgattaacc gttggttatc ctggtacatt gcgaacaggt
    14101 agttgttata actcttccct ttagtcgagt agcccttcag cttgtgaatc atcgctgtgg
    14161 cagtcacttc ctgatccgct ttacgcagct gtgcacgcga tttacggaac gaagagtaag
    14221 ccggatgagt attcaggtta gtgacatagg cgctcaccga ttctttgaca gaactaaact
    14281 gtgagtaccc tttcacttta cccggcgcat tggtacaacg tcctttcatg catttcatgc
    14341 cgaacagatt gttgttgttg cgcgccagct ttgacgttcc ccaaccgctt tctgctgcag
    14401 ccatcgtcgc caccatactg gtagggataa tgtctacgcg ttcaagcaag gtattccacg
    14461 ggatttttcg cgtattacca gaccacttca ccttgtagcg tttggcgatg tctttcagac
    14521 gcgcacgctc agcaggtgac cattggccct gatactgttt tgaaatcagc cagttacgtt
    14581 ccgcagtaat ggccgcattt tggctggtaa tgtaaggcat tacagtccgg agaaacgcct
    14641 ttttccttgg tgttccggaa gggtattttc gcaaatcagg aagtgaacta ctctttgcac
    14701 tattgcgaga atactcttgt ttactgctta cctgtttatt actagcttta gttaagtggg
    14761 acttttgact cgctgttgtt gtgtgcgtct tcgctaacac ctcactcgaa aacaccagag
    14821 tgagtaacat aagaatcatc gccccatatc gtcgtatggg agtcaaaatc atcaggtctc
    14881 ctggtcggat ttaatcattc caacacctta tatttttcac aaatttgaga gttgaatctc
    14941 aaatcatatc aaaaatagct gtcaagagca ccccaaggaa tagtccaaat ctgaaactat
    15001 gtcacgagtt aacgattcag attggcgcta aatcgcagaa aatgtggggt ttatcgcaaa
    15061 attcagccgt tttttgtgcg cgatcgctca cccttgcttc tcatcctgtg gacttaccgc
    15121 tcagggatga gttttgtctg gcttatcgct ggcaaactgt ctgaaatcgc agcaataagg
    15181 actcatccgc catgaaactc gccgcctgtt ttctgacact ccttcctggc ttcgccgttg
    15241 ccgccagctg gacttctccg gggtttccca cctttagcga acaggggacg ggaacatttg
    15301 tcagccacgc gcagttgccc aaaggtacgc gtccactaac gctaaatttt gaccaacagt
    15361 gctggcagcc tgcggatgcg ataaaactca atcagatgct ttccctgcaa ccttgtagca
    15421 acacgccgcc tcaatggcga ttgttcaggg acggcgaata tacgctgcaa ctagacaccc
    15481 gctccggtac accaacattg atgatttcca tccagaacgc cgtcgaaccg gtagcaagcc
    15541 tggtccgtga atgcccgaaa tgggatggat taccgctcac actggatgtc agcgccactt
    15601 ttccggaggg agccgccgtc cgggattatt acagccagca aattgcgata gtgaagaacg
    15661 gtcaaataac gttacaaccc gctgccacca gcaacggttt actcctgctg gaacgggcag
    15721 aaactgacgc ccctgcccct ttcgactggc ataacgccac ggtttacttt gtgctgacag
    15781 atcgtttcga aaacggcgat cccagtaatg accagagtta cggacgtcat aaagacggta
    15841 tggcagaaat tggcactttt cacggcggcg atttacgcgg cctgaccaac aaactggatt
    15901 acctccagca gctgggcgtc aatgctttat ggataagtgc cccatttgag caaattcacg
    15961 gctgggtcgg cggcggtaca aaaggcgatt tcccgcatta tgcctaccac ggttattaca
    16021 cacaggactg gacgaatctt gatgccaata tgggcagtga agccgatcta cggacactgg
    16081 ttgatagcgc gcatcagcgc ggtattcgta ttctctttga tgtcgtgatg aaccacaccg
    16141 gctatgccac actggcggat atgcaggagt atcagtttgg cgcgttatat ctttctggtg
    16201 acgaagtgaa aaaaacgttg ggtgaacgct ggagcgactg gaaacctgcc gccgggcaaa
    16261 cctggcatag ctttaacgat tacattaatt tcagcgacaa aacaggctgg gataaatggt
    16321 ggggaaaaaa ctggatcaga accgatatcg gcgattacga caatcctgga tttgacgatc
    16381 tcactatgtc gctggccttt ttgccggata tcaaaaccga atcaacgacc gcttctggtc
    16441 tgccggtgtt ctataaatac aaaacggata cccacgctaa agtcatcgac ggctttaccc
    16501 ctcgcgatta cttaacccac tggttaagtc agtgggttcg cgactatggg attgatggtt
    16561 ttcgggtcga caccgccaaa catgttgagt tgcccgcctg gcagcaactg aaaaccgaag
    16621 ccagcgccgc gcttgtcgaa tggaaaaaag cgaatcccga taaagcgtta gatgacaaac
    16681 cgttctggat gaccggtgaa gcctggggcc acggcgtgat gcaaagtgac tactatcgcc
    16741 acggcttcga tgcgatgatc aatttcgatt atcaggagca ggcggctaaa gcagtcgatt
    16801 gtctggcgca gatggatacg acctggcagc aaatggcgga gaaattgcag gatttcaacg
    16861 tgttgagcta cctctcgtcg catgataccc gcctgttccg tgaagggggc gacaaagcag
    16921 cagagttatt attagcgcca ggcgcggtac aaatctttta tggtgatgaa tcctcgcgtc
    16981 cgttcggccc caccggttcg gatccactgc aaggcacgcg ttcggatatg aactggcagg
    17041 atgttagcgg caaatctgcc gccagcgtcg cgcactggca gaaaatcagc cagttccgcg
    17101 cccgccatcc cgcaattggc gcgggcaaac aaacgacact ttcgctgaag cagggctacg
    17161 gctttgttcg tgagcatggc gacgataaag tgctggtcat ctgggctggg caacagtaac
    17221 ttttccggct tcccgttcgt cagtacctcg ggaagccgcc aaccaggata aaatgtcaga
    17281 cctaatcagc gttgcaggat aaagcaccgc tccctcttca acagagcgat ttgcacccca
    17341 gcaaatgtag cgttattgtt accttccttg ctacagagtt cgacagatat cccgctatga
    17401 cattctccct ttttggtgac aaatttaccc gccactccgg cattacgctg ctgatggaag
    17461 atctgaacga cggtttacgc acgcctggcg cgattatgct cggcggcggt aatccagcgc
    17521 agatcccgga aatgcaggac tacttccaga cgctactgac cgacatgctg gaaagtggca
    17581 aagcgactga tgcactgtgc aattacgacg gtccacaggg gaaaacggag ctactcacac
    17641 tgcttgccgg aatgctacgc gagaagttgg gttgggatat cgaaccacag aatattgcac
    17701 taacaaacgg cagccagagc gcgtttttct acttatttaa tctgttcgcc ggacgccgtg
    17761 ccgatggtcg gatcaaaaaa gtgctgtttc cgctggcacc ggaatacatt ggctacgctg
    17821 acgccggact ggaagaagat ctgtttgtct ctgcgcgtcc gaatattgag ctgctgccgg
    17881 aaggccagtt taaataccac gtcgattttg agcatctgca tattggcgaa gaaaccggga
    17941 tgatttgcgt ctcccggccg acgaacccaa caggcaatgt gattactgac gaagagttgc
    18001 tgaagcttga cgcgctggcg aatcaacacg gcattccgct ggtgattgat aacgcttatg
    18061 gcgtcccgtt cccgggtatc atcttcagcg aagcgcgccc gctgtggaat ccgaatatcg
    18121 tactgtgcat gagtctttcc aagctgggtc taccaggctc ccgctgcggc attatcatcg
    18181 ccaatgaaaa aatcatcacc gccatcacca atatgaacgg cattatcagc ctggcacctg
    18241 gcggtattgg tccggcgatg atgagtgaaa tgattaagcg taacgatctg ctgcgcctgt
    18301 ctgaaacagt catcaaaccg ttttactacc agcgtgttca ggaaactatc gctatcattc
    18361 gccgctattt accggaagat cgctgcctga ttcataaacc ggaaggagcc attttcctct
    18421 ggctatggtt taaggatttg cccattacga ccgagcagct ctaccagcgc ctgaaagcac
    18481 ggggcgtgct gatggtgccg gggcacaact tcttcccagg gctggataaa ccgtggccac
    18541 atacacatca atgtatgcgc atgaactacg tgccggagcc ggagaaaatt gaggcgggtg
    18601 tgaagattct ggcagaagag atagaaagag cctgggctga aagtcactaa aaattgcctg
    18661 atgcgctacg cttatcaggc ctacatgatt tctgcaatat attgaatttg gtagaatttg
    18721 taggccggat aaggcgtttt agccgcatcc ggcataaaca aagcgcacta tccctgcgtt
    18781 gagaaacgcc ggaaaaacgt ttcaaacagg ctgctgccgt agccgatgtc gctgcacttt
    18841 ctcgacatcc atgcactgca acgcctttgt cgggcaggct tcaacacagg ccggtcccgt
    18901 cgcccgatgc cagcacagat cgcacttaat cgccctcgcc tttttgcgcg aagaaacgac
    18961 ctccatcgca ccaaacgggc aagccagcat acagctttta cagccaatgc aacgtgattg
    19021 ttcaacgaaa atatgcccat gttcgcggct tatcgcgtca acagggcaga cattcgcgca
    19081 cggtgcatct tcacactgat ggcaggctac agccgtggtc catgtgtggt ctttaatgac
    19141 acgaatacgg gaaataaact cgtctggtga caacgcagcg caatcctgat tctcctgatg
    19201 cgacactgcg caagccactt cacaggtacg gcaaccgata catttcgtcg catccgcaat
    19261 aataaaccgg ttcatgatca ctcctttgca ctaaaacagc agagtgccag agagcgcgtc
    19321 tctactgctt tgatccaacg aaggaaatgt taaagttttg gcacttgtcc aaagcgttat
    19381 gtgatcgcgc cctggtcgtc cctgacagta aatcccagtt cattagaaat agcctgcgcg
    19441 gtttcacgca gtggtttcag gagatttttc tcccccacct gtttcagacg tgatgtcgaa
    19501 agcgaaatcg acacggcgta cggcacccgc ccatgaatat caaacaccgg aacagcaata
    19561 caggagacgc cgagttcgtt ttcttctctg tccatcgccg ctccgctttc acgaatgtgc
    19621 gccagttcgt cgaacatcgc gggcagctcg gtaatggtat tgcgggttaa cggctggatc
    19681 tcatgctgat ggctttccca gtatgacttc acgtagtccg ggtggccaaa cgccatgtag
    19741 atcttgccca ttgcggaaca gtagagcggc atatgctggc caatataggc acgggttcgc
    19801 agcatcccgg ttgtgggttc cagcttataa atcaaaatag cgtgatcgtc ttcgcggctg
    19861 gagaagttaa tagtttcacc agtggcgatg ttcagtgcct caagatgcgg agcggcgata
    19921 tgaatgatat tcagcgaaga cagcgccttc tgcccgacgg caataaattt ggtggtcagg
    19981 cgataactcc ctgcggcggg cgcggtggtc acatagccgc aggattgtaa tccctgcaat
    20041 aagcgatgga cggtactctt atttaaacca gccagctccg aaagatgcgc caacggacaa
    20101 ccgtttggat agttgctcaa aatctcaatc agcatcaacc cgcgaaacag actctggctt
    20161 ccggctggac gctctttttc ctgcgccatc tcgttctctt tttttcccat cacttctttc
    20221 cccattttgt cgcgtcaaga tggtagcgca aagtgtgtcg tagttcacga tctcgacaga
    20281 taatttataa ccacttgatt tttaagtttt ttgaaattca tcaatcagat tgctttgtta
    20341 aaaagcgatc gatatatttg aaatcagatt tcgcatattg aaattttaag ccaaaaaagc
    20401 gatcaaaaaa acaaggaagc cagggatgaa agtgacattt gagcagttaa aagcagcctt
    20461 taatcgagtc ttaatttcac gcgacgttga cagcgaaacg gctgatgcct gtgccgaaat
    20521 gttcgcccgc accaccgaat ccggcgttta ttctcacggc gtcaaccgtt tccctcgttt
    20581 cattcaacaa ctggaaaacg gcgatatcat tcctgatgcc caacccaaac gtataaccag
    20641 cctcggcgca attgaacagt gggacgccca gcgttcgatc ggtaacctga cagcgaaaaa
    20701 gatgatggat cgcgccattg aactggctgc cgatcacggt attggtctgg tggcactacg
    20761 taatgccaac cactggatgc gcggcggcag ctacggctgg caggcggcgg aaaaaggcta
    20821 tattggcatt tgctggacca actccatcgc cgtaatgccg ccgtggggcg caaaagagtg
    20881 tcgcatcggc actaacccgc tgatcgtcgc cattccttct accccaatca ccatggtcga
    20941 tatgtcgatg tcgatgttct cttacggcat gttagaagtt aaccgtctgg caggccgtca
    21001 gctcccggtc gatggtggct ttgatgatga gggcaatttg accaaagaac ctggcgttat
    21061 cgagaagaat cgccgcattt tgccgatggg ttactggaaa ggttcgggca tgtcgattgt
    21121 gctggatatg atcgccactc tcctttctga cggcgcatcg gttgccgaag tcacccagga
    21181 caacagcgac gaatacggca tttcacaaat ttttattgcc attgaagtag ataagcttat
    21241 cgacggcccc acccgcgatg ccaaactgca acgcatcatg gattacgtta ccaccgctga
    21301 acgcgctgac gaaaatcagg ccattcgctt acccggccac gaatttacta ccctgctggc
    21361 cgaaaaccgc cgtaacggta ttaccgttga tgacagcgtg tgggcaaaaa tccaagcgtt
    21421 ataaggagat aacccatgat ttttggacat atcgcgcagc ctgacccgtg ccgtttgcct
    21481 gccgccattg aaaaggcgct tgattttctg cgcaccaccg acttcaacgc cctggaaccg
    21541 ggcgttgtcg aaatcgacgg caagaatatt tatgcgcaaa ttatcgattt aaccacacgc
    21601 gaagcggcgg aaaaccgtcc ggaagtgcat cgtcggtata tcgatattca gtttctggcg
    21661 tggggtgaag agaaaatcgg cattgctatt gatacgggaa ataataaagt cagcgaatca
    21721 ctattagagc agcgcgatat tattttttat cacgatagtg aacatgaatc atttatagaa
    21781 atgataccgg gcagctacgc tattttcttc ccgcaggatg ttcaccgacc aggttgtatt
    21841 ctgcaaacaa cctctgagat acgaaaaatt gtggttaaag tcgcattaac agcgctgaat
    21901 taatcgaata atcgtctacg ccagaacgcc tgggtaatgt tattgctcta actatattaa
    21961 taatttaggt tgctgcacat gcaacttgaa ttatgacaga catagcctca aggaatagct
    22021 atgaaaaaaa tacttgaagc aatactggcg attaatctcg ccgtactttc ctgtattgtg
    22081 tttataaaca ttattttaag atatggtttt cagacaagca ttttatctgt agatgaattg
    22141 tcacgttatt tatttgtctg gttaacattt attggcgcga ttgtggcttt tatggataac
    22201 gcccacgttc aggtcacctt tctggtggag aaactctccc ccgcatggca gcgacgagtc
    22261 gctctggtta cccattcctt aatcttattt atttgtggcg cactggcctg gggcgcaacg
    22321 ctaaaaacaa ttcaggactg gagcgattat tcaccgatcc tcggtttgcc catcggcctg
    22381 atgtatgccg cttgtctgcc caccagcctc gttatcgcat tctttgaact gcgtcattta
    22441 tatcaactca tcacgcgtag caattctcta acgtcgccac cgcaaggagc ttaatcatgg
    22501 ctgtgctgat ttttctgggc tgtctgttgg gtggtattgc tatcggtttg cctattgcct
    22561 gggcactgtt gttgtgcgga gcggcgttaa tgttctggct ggacatgttt gatgtccaga
    22621 tcatggcaca aacgctggtg aacggagccg acagcttctc cctgctggcg attccgttct
    22681 ttgtgctggc gggtgaaatc atgaatgcgg gcggcttgtc aaaacgcatt gttgacctgc
    22741 cgatgaaact ggtggggcat aaacctggcg ggttgggcta cgtcggcgtg ctggcggcta
    22801 tgattatggc gagcctttct ggttctgctg ttgctgatac cgccgccgtt gccgcgctat
    22861 tagtgccgat gatgcgcagc gctaactacc cggtcaaccg ggctgcgggg ctgattgcct
    22921 ctggcggcat tatcgcgcca attattcccc cttccattcc gtttattatt ttcggcgttt
    22981 ccagcggatt atccatcagt aagctgttta tggcgggcat tgcccctggc atgatgatgg
    23041 gcgcaacgct gatgcttacc tggtggtggc aggccagtcg ccttaattta ccccgccagc
    23101 aaaaagcaac gatgcaggaa atctggcact cttttgtctc cggtatctgg gcgctgtttc
    23161 ttccagtcat cattattggc gggtttcgct ccggtctgtt tacccctacc gaagccgggg
    23221 ccgttgctgc tttctatgcg ctgtttgtcg ccacagttat ttaccgtgaa atgaccttcg
    23281 ccacactttg gcatgtgctc attggcgcag cgaaaacgac ctcagtggtg atgtttctgg
    23341 tagcctcggc acaagtttcc gcctggctga tcaccattgc tgaactgccg atgatggttt
    23401 ctgatttact gcaaccgctg gtcgattcac cgcgtctgct gtttatcgtc attatggtgg
    23461 ctatcctgat tgtcggcatg gtcatggatt taacgccgac ggtattaatt cttaccccgg
    23521 tattaatgcc tttagttaaa gaggcaggaa tcgatccgat ttatttcggt gtcatgttta
    23581 tcattaactg ctcaatcggc ttaattacac cgcctatcgg taatgtgctt aacgttattt
    23641 caggggtggc aaaactcaaa ttcgatgatg cagtcagagg cgttttccct tacgtcctgg
    23701 ttttatattc attattagtc gtatttgttt ttattcccga tctcatcatc ctccctttga
    23761 aatggattaa ttaaaaggaa aatattatga aattacgctc tgtaacctac gcattattca
    23821 ttgctggcct ggctgcattt agtacatctt cactggcggc gcaatcttta cgtttcggtt
    23881 atgaaacctc acaaaccgac tcgcaacata ttgcggcgaa aaaattcaat gatttattgc
    23941 aggagagaac caaaggcgag ctgaaattaa aactgttccc ggacagtact ctcggtaacg
    24001 cacaggcgat gatcagcggc gtacgtggcg gcaccatcga tatggaaatg tccggctcga
    24061 ataactttgc cgggttatca ccagtgatga acttgcttga tgtccctttc ctgttccgcg
    24121 ataccgctca cgcgcataaa acgctcgacg gcaaagtcgg tgatgatctg aaagcctcac
    24181 ttgaaggtaa aggactaaaa gtactggcct actgggaaaa cggctggcgc gatgtcacca
    24241 actcgcgcgc accggttaaa acccccgccg acctgaaagg gctgaaaatt cgcaccaaca
    24301 atagcccgat gaatatcgcc gcattcaaag tctttggcgc taacccgatc ccgatgccgt
    24361 ttgccgaagt ctataccggg ctggaaacgc gcactatcga cgctcaggaa cacccgatca
    24421 acgtcgtctg gtcagcaaaa tttttcgaag tacagaagta cctttctctg acgcaccacg
    24481 cctattcgcc acttctggtg gtgatcaaca aagcgaagtt tgatggctta agcccggagt
    24541 tccagcaggc gctagtttca tctgcacagg aagcgggtaa ctaccagcgc aaactggttg
    24601 ccgaagatca gcaaaaaatc atcgacggca tgaaagaagc gggcgtggaa gtcatcaccg
    24661 atctcgaccg caaagccttt agcgacgcac tgggtactca ggtccgcgac atgtttgtta
    24721 aagatgttcc gcagggcgct gagctgctga aagccgtgga tgaggtgcaa taaaccatga
    24781 cgcaatactg gctggggtta gattgtggcg gtagctggct gaaagccggg ctgtatgacc
    24841 gcgaaggccg ggaggcaggc gtgcagcgcc tgccgctgtg cgcattaagc ccgcagccag
    24901 gttgggcaga gcgcgatatg gcagaactgt ggcaatgctg catggctgtc attcgcgccc
    24961 tgcttactca ttctggtgtt agcggggaac aaattgtcgg tatcggcatc tccgcacagg
    25021 gaaagggctt gtttttgctg gataaaaacg acaagccgct cggtaacgcc attttgtcct
    25081 cggatcgccg ggcgatggaa atcgttcgtc gctggcagga agatggcatc ccggaaaaac
    25141 tctacccgct gacgcgacaa accttgtgga ccgggcatcc ggtgtcgctg ttacgctggc
    25201 tgaaagagca cgaaccggaa cgctacgcgc aaattggctg cgtgatgatg acgcacgact
    25261 acctgcgctg gtgtttaacc ggcgtcaaag gttgcgaaga gagcaatatt tctgagtcca
    25321 atctctacaa catgagtctt ggggaatatg acccgtgcct caccgactgg ctggggatcg
    25381 ctgaaatcaa ccacgccctg ccacttgttg tcggatctgc cgaaatctgc ggggagatca
    25441 ccgctcagac agccgcactg accggtctga aagcgggtac gcccgttgtc ggcggcctgt
    25501 ttgatgtggt ttccaccgca ctctgcgccg ggatcgaaga cgaatttacc ctcaatgcgg
    25561 tgatgggcac ctgggcggtg accagcggca taacccgtgg tttacgtgac ggtgaagcgc
    25621 atccgtatgt ctatggtcgc tacgttaacg atggtcaatt tatcgttcat gaagcaagcc
    25681 ccacctcttc cggcaatctc gaatggttta ccacacagtg gggagaaatc tcgtttgatg
    25741 agatcaacca ggccgttgcc agcttgccga aggccggggg cgatctcttt ttcctgccgt
    25801 tcctgtacgg cagcaacgct ggactcgaga tgaccagcgg tttctacggg atgcaggcca
    25861 ttcacacccg cgtacacctg ttgcaggcca tttatgaagg cgtggtgttc agccatatga
    25921 cccatctcaa ccggatgcgc gaacgtttta ctgatgtgca caccctgcgc gtcactggcg
    25981 gtccggcgca ctccgatgtc tggatgcaaa tgctggcgga cgtcagcggt ctgcgtatcg
    26041 agctgccgca ggtggaagaa accggctgct ttggtgcggc acttgccgcc cgcgtcggca
    26101 ccggagttta tcgcgatttc agcgaagccc aacgtgattt acagcacccg gtacgcaccc
    26161 tgctgccgga tatgaccgcc catcagcttt accaacaaaa ataccaacgt tatcagcatc
    26221 tcattgccgc acttcagggc tttcacgccc gcatcaagga gcacacatta tgagccgacc
    26281 acttctgcaa ctggccctcg accactcatc acttgaagac gcacagcgcg acgtgacgct
    26341 gttaaaagac agcgtcgata tcgtcgaagc gggcaccatt ctctgtttaa acgaagggct
    26401 tggcgcagtg aaagccttgc gcgaacagtg cccgaacaaa atcatcgtcg ctgactggaa
    26461 ggtcgccgat gccggtgaaa cgctcgcgca acaggcgttt ggcgcaggcg ctaactggat
    26521 gaccatcatc tgcgccgcac cgctcgcgac ggtggaaaaa ggccacgcga tggcgcaacg
    26581 ctgcggtggt gaaattcaga tcgagttgtt cggtaactgg acgctggacg acgcccgcga
    26641 ctggcatcgt attggcgtgc ggcaggccat ttatcatcgc ggtcgcgacg cgcaggccag
    26701 cgggcaacag tggggcgagg ccgatctggc acgcatgaag gcgctttccg atatcggcct
    26761 tgagctttcc attaccggcg ggattacccc tgctgacctg ccgctgttta aagatattcg
    26821 cgtgaaagcg tttattgccg ggcgagcact ggcaggcgca gcaaatccgg tgcaggtggc
    26881 tggcgatttc catgcacaaa ttgatgccat ctggggaggt gcgcgtgcgt aatcatccgt
    26941 tagggattta tgaaaaagcg ctggcgaaag atctctcctg gccggagcgg ctggtgctgg
    27001 caaaaagctg tggctttgat tttgtcgaaa tgtcggtgga tgaaaccgac gaacggctct
    27061 cacgtcttga ctggagcgcc gcacaacgtg cttcgctggt cactgcgatg atcgaaacgg
    27121 gtgttgggat cccgtcgatg tgcctgtccg cacatcgtcg ctttcctttt ggtagccgtg
    27181 acgaggcagt gcgcgaacgg gcgcgggaaa tcatgagtaa agcgattcgc ctggcgcgcg
    27241 atctcggcat tcgcaccatc cagctggcgg gctacgacgt ctattacgaa gaccacgacg
    27301 aaggcacccg gcaacgtttt gctgaagggc tggcgtggac agtcgaacag gcggcggcat
    27361 cacaagtaat gctggcggtg gagattatgg atactgcgtt tatgaactcc atcagcaaat
    27421 ggaaaaagtg ggacgagatg ctcgcctcgc cgtggttcac cgtctacccg gacgtcggca
    27481 acctcagcgc ctggggcaat gatgttcccg ccgaactgaa actgggcatt gaccgtatcg
    27541 ccgcgatcca cctgaaagat actcaaccag tcaccgagca aagccccgga cagttccgtg
    27601 atgtgccgtt tggcgaaggc tgcgtcgatt tcgttggcat cttcaaaacg ctgcatgaac
    27661 tgaactatcg cggttctttc ctgattgaga tgtggaccga aaaagccaaa gagccggtgc
    27721 tggagattat tcaggcgcgg cgctggattg aagcgcgtat gcaggaggca ggttttatat
    27781 gttagagcaa ctgaaagccg acgtgctggc ggcaaatctg gcacttcccg ctcaccatct
    27841 ggtgacgttc acctggggca atgtcagcgc ggtagacgaa gcgcggcaat ggatggtaat
    27901 caaaccttcc ggcgtcgagt acgacgtgat gaccgccgac gatatggtgg tggttgagat
    27961 agccagcggt aaggtggtgg aaggcagcaa aaagccctcg tccgacacgc caacgcatct
    28021 ggcgctttac cgtcgctatg ccgaaatcgg tggcattgtg catacccact cgcgccacgc
    28081 caccatctgg tcgcaggccg ggctggatct ccccgcctgg ggcaccacgc acgccgatta
    28141 tttctatggt gccatcccct gcacgcgaca gatgaccgca gaggagatta acggcgaata
    28201 tgaatatcag accggcgaag tgatcattga aactttcgaa gaacgcggca ggagtccggc
    28261 acaaatcccg gcggtgctgg tgcattctca cggcccgttc gcctggggaa aaaatgccgc
    28321 cgatgcggtg cataatgccg tagtgctgga agagtgcgcc tatatgggcc tgttctcgcg
    28381 ccagcttgcg ccgcagctcc ctgcgatgca aaacgaactg ttagataagc actacctgcg
    28441 taagcatggg gccaatgcct attacgggca gtaatccctc acgccggggc ttcattgccc
    28501 cggcactacg aattgatatg ttccttgctg taacgtcgct tccacgcagc tggcgttaaa
    28561 ccggtgtgtt tgcgaaaaat ctgtcggaaa tagccaacgt cattaaaccc gcacaacgca
    28621 gccacttcag tgagcgatcg cgcttcgcta attaacagct tttcggcttc gcgcactcgc
    28681 tggcgatgaa ttgcttctgt taacgtcaga tgaaacaccc gccggtagac gcgcccaaga
    28741 taatcggcat tgcagtacag ctcgcgggct aaaagcgccg tagaaagcgg gcgatgatat
    28801 tgcgtgcgaa tcagtttctg cgctttccag gcaagcgtgg tacctgcggc atcggcctgt
    28861 tcatcaagac gcgcgtcacg ggtgagctgc tgcaaaatca gcagcacaat aaactccagc
    28921 gccggagtgc gttgatgctt ttcctgttcg cttaagaact ggcgaaacag ggccaccagc
    28981 gcctgcggat cggcaacctt cgtttgttgc ggcacgctaa gatgagtgag ccagcgcggg
    29041 tcgctatccg gcgctttaac ctcaaaatgc agccagtaaa atttgagatc ggcgggaaaa
    29101 tcctctaccc caacatgctg tcggttcggc cagagtaata aactctctcc cgcatcgacc
    29161 agaaatatcc gctcttcttc ctgaatcgtt aatcgacctt tttcgacaaa gataatttcc
    29221 catgactcca gccgccttgc cgggtgcctc cctacgccac gggaaataaa taatccacca
    29281 ttttgcactt taatcggtaa cgtcatggat aattcgagca ttgatatgta atttcccgct
    29341 tattcattaa tga
//
LOCUS       DAZGAP010000042        29311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGAP010000042 DAZGAP010000000
VERSION     DAZGAP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.042"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..355)
                     /locus_tag="ACHLAM_001562"
                     /pseudo
     CDS             complement(89..355)
                     /locus_tag="ACHLAM_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(631..1947)
                     /gene="hipA"
                     /locus_tag="ACHLAM_001563"
     CDS             complement(631..1947)
                     /gene="hipA"
                     /locus_tag="ACHLAM_001563"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO7853114.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(1947..2213)
                     /gene="hipB"
                     /locus_tag="ACHLAM_001564"
     CDS             complement(1947..2213)
                     /gene="hipB"
                     /locus_tag="ACHLAM_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO7853115.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(2406..4295)
                     /locus_tag="ACHLAM_001565"
                     /pseudo
     CDS             complement(2406..4295)
                     /locus_tag="ACHLAM_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(4445..6037)
                     /gene="lsrK"
                     /locus_tag="ACHLAM_001566"
     CDS             complement(4445..6037)
                     /gene="lsrK"
                     /locus_tag="ACHLAM_001566"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO7853116.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDPEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVNELKELHNNTFENKVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLIDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(6116..7069)
                     /gene="lsrR"
                     /locus_tag="ACHLAM_001567"
     CDS             complement(6116..7069)
                     /gene="lsrR"
                     /locus_tag="ACHLAM_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO7853117.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            7318..8853
                     /gene="lsrA"
                     /locus_tag="ACHLAM_001568"
     CDS             7318..8853
                     /gene="lsrA"
                     /locus_tag="ACHLAM_001568"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO7853118.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGD
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            8847..9875
                     /gene="lsrC"
                     /locus_tag="ACHLAM_001569"
     CDS             8847..9875
                     /gene="lsrC"
                     /locus_tag="ACHLAM_001569"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO7853119.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTVLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            9875..10867
                     /gene="lsrD"
                     /locus_tag="ACHLAM_001570"
     CDS             9875..10867
                     /gene="lsrD"
                     /locus_tag="ACHLAM_001570"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO7853120.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            10879..11901
                     /gene="lsrB"
                     /locus_tag="ACHLAM_001571"
     CDS             10879..11901
                     /gene="lsrB"
                     /locus_tag="ACHLAM_001571"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO7853121.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            11928..12803
                     /gene="lsrF"
                     /locus_tag="ACHLAM_001572"
     CDS             11928..12803
                     /gene="lsrF"
                     /locus_tag="ACHLAM_001572"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO7853122.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            12827..13117
                     /gene="lsrG"
                     /locus_tag="ACHLAM_001573"
     CDS             12827..13117
                     /gene="lsrG"
                     /locus_tag="ACHLAM_001573"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO7853123.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            13174..13932
                     /gene="tam"
                     /locus_tag="ACHLAM_001574"
     CDS             13174..13932
                     /gene="tam"
                     /locus_tag="ACHLAM_001574"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO7853124.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(13936..14850)
                     /gene="yneE"
                     /locus_tag="ACHLAM_001575"
     CDS             complement(13936..14850)
                     /gene="yneE"
                     /locus_tag="ACHLAM_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO7853125.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(15047..16498)
                     /gene="uxaB"
                     /locus_tag="ACHLAM_001576"
     CDS             complement(15047..16498)
                     /gene="uxaB"
                     /locus_tag="ACHLAM_001576"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO7853126.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTGLVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            complement(16726..18144)
                     /locus_tag="ACHLAM_001577"
     CDS             complement(16726..18144)
                     /locus_tag="ACHLAM_001577"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7853127.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(18283..18642)
                     /gene="yneG"
                     /locus_tag="ACHLAM_001578"
     CDS             complement(18283..18642)
                     /gene="yneG"
                     /locus_tag="ACHLAM_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO7853128.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(18642..19568)
                     /gene="glsB"
                     /locus_tag="ACHLAM_001579"
     CDS             complement(18642..19568)
                     /gene="glsB"
                     /locus_tag="ACHLAM_001579"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO7853129.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRMGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCIELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(19632..21020)
                     /gene="sad"
                     /locus_tag="ACHLAM_001580"
     CDS             complement(19632..21020)
                     /gene="sad"
                     /locus_tag="ACHLAM_001580"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO7853130.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNNGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            21121..21996
                     /gene="yneJ"
                     /locus_tag="ACHLAM_001581"
     CDS             21121..21996
                     /gene="yneJ"
                     /locus_tag="ACHLAM_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7853131.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAATKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPAMLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            22080..22199
                     /gene="yneK"
                     /locus_tag="ACHLAM_001582"
     CDS             22080..22199
                     /gene="yneK"
                     /locus_tag="ACHLAM_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO7853132.1"
                     /translation="MVTPVSISNFISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            22341..23531
                     /gene="ydeA"
                     /locus_tag="ACHLAM_001583"
     CDS             22341..23531
                     /gene="ydeA"
                     /locus_tag="ACHLAM_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO7853133.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGELITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(23556..24221)
                     /gene="marC"
                     /locus_tag="ACHLAM_001584"
     CDS             complement(23556..24221)
                     /gene="marC"
                     /locus_tag="ACHLAM_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO7853134.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            24433..24867
                     /gene="marR"
                     /locus_tag="ACHLAM_001585"
     CDS             24433..24867
                     /gene="marR"
                     /locus_tag="ACHLAM_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO7853135.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            24888..25271
                     /gene="marA"
                     /locus_tag="ACHLAM_001586"
     CDS             24888..25271
                     /gene="marA"
                     /locus_tag="ACHLAM_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO7853136.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            25303..25521
                     /gene="marB"
                     /locus_tag="ACHLAM_001587"
     CDS             25303..25521
                     /gene="marB"
                     /locus_tag="ACHLAM_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO7853137.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(25552..26451)
                     /gene="eamA"
                     /locus_tag="ACHLAM_001588"
     CDS             complement(25552..26451)
                     /gene="eamA"
                     /locus_tag="ACHLAM_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO7853138.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            26646..27833
                     /gene="ydeE"
                     /locus_tag="ACHLAM_001589"
     CDS             26646..27833
                     /gene="ydeE"
                     /locus_tag="ACHLAM_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO7853139.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRNEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            27960..28055
                     /gene="mgtS"
                     /locus_tag="ACHLAM_001590"
     CDS             27960..28055
                     /gene="mgtS"
                     /locus_tag="ACHLAM_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO7853140.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            28055..28159
                     /gene="mgtT"
                     /locus_tag="ACHLAM_001591"
     CDS             28055..28159
                     /gene="mgtT"
                     /locus_tag="ACHLAM_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO7853141.1"
                     /translation="MNGDNPSPNRPLVTTVYKGPDFYDGEKKPPVNRR"
     gene            complement(28274..29164)
                     /gene="dgcZ"
                     /locus_tag="ACHLAM_001592"
     CDS             complement(28274..29164)
                     /gene="dgcZ"
                     /locus_tag="ACHLAM_001592"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO7853142.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         7324 a         7474 c         7038 g         7475 t
ORIGIN      
        1 ttatatggct ataaatcatt agccatagtt aatgtttgcc tggtgtaact aacacggaat
       61 tgctctaaac agataagtgt agggtttatt aaagctgacc gacagacttt cttaacctgg
      121 taatatctct cttaggtgac tctccaaaca tccgcgaata ttcccgactg aaatgggata
      181 agctttcgta accgacagca taggctgcag tggtgacatc gtaatgttca ttcagcatca
      241 gtcgcctggc ttcattgagg cttagccatt tccgataccg gagtggactc attcccgcaa
      301 gctgtctgaa atgttgatgg aaagaggggg tgttcatttg cacgcggttt gccagcctga
      361 ctcttcaacg ctgagaagat gcggcaaacg gcaaacctgc ataccagcca gcctatatcc
      421 ggtatgcaca ggccaattat gaaagaggag aggctttatg ttctggaacc ttgaagtaat
      481 aacgtcgacc ggaaaatcac tctgcatcat cactcagatt caatttaaca taatatacat
      541 tatgcgcacc aacataaacc aagggagaat ccagccgttg gcggtcatga ttgtcctgct
      601 cattaacaac ggccacaccc catatcttac ttaataccat tctcggctta accgacggtg
      661 caacctcaac acattggttt caactgccgt caccacgttc tccggaaaat ctgtcggtaa
      721 tgaagtcttc acgttatcca gtgctgccgg aatcattctg gcaaagtcac tcaggatttc
      781 atgcatctgc acttgcggaa atttcagcac ctttgctgtc gccagaaaat gtcgcggata
      841 aattttatcg attgccgttt ttttgccttt ggatgcgtta agccccattg ccagtttgag
      901 atcgctgatg tgtattcctg caccacccaa taccggaaat gctgaaatga tgtcgtaaaa
      961 tggcgtgagt cgataactgc cgccagcctg aataaatacg gagaagtttt ttgcatggcc
     1021 atccgttgcg ccaatcaacc actggaacac ctgaaacttc ataaaatcat agcgatcttt
     1081 cagcgcctcg ctggacccca tcaaaaaagc catgatccgc gcgatgcctg ggcctccatc
     1141 tgattcatat ttcaccgatg aaggtaaacc gaatgtctga cacatatcct cctgtggcaa
     1201 gcgaagtaaa accgttcgct cagcattcca acgcctgtca aaacgttcga ccgctaacgc
     1261 gcgcacattt cccgctttaa tgatttctgc gtccggcaca ttcaacccaa gttctttagc
     1321 cagcagcaga cagtaatact cattatcaac gctttggctg agatcgagcg tcgcattggg
     1381 ctgcctgatt tcgccaatcg gtaatttaat gatgtgcgtc gtcggcgtta ttcctttcgg
     1441 aatgcaccag tcattgccta ttctgagcag cgctgtcttc tcctgcgcgc cagcaaccga
     1501 gatgcgaaag tcattttctt ctctaatcat gcccagaggg atatctgctt tataagccgt
     1561 taatacttct tcaagcctgg cttcagtgag ctgttcccat gccattatcg ggcgcgttat
     1621 ggtttcattt tcgggtaata acgttacggc accaacgctg tctcgcccta tttctgacaa
     1681 taaatcaaac ggttgtctgg atttagcatg ataacgttta acgatccgat cacgtacaat
     1741 cgggctatcg ggtaacaggt tatcgaagaa gttaaatacg gcatcagagg tgatattccc
     1801 cctctgcaat ggcagcgaaa gtgacaacgg tctggcataa cggctggcta accactccgg
     1861 tgcatactta aaggtgtgcg cgccattggc taacttcgtt aactcgccta cccgctggtt
     1921 gttcatccaa gtgacaagtt taggcattac cactccagat cttgctgttc tgttgattct
     1981 ggcgaggcat ttttcgtgtc gcatagcgtc attgagagtt caagcgactg taaaatctta
     2041 aaaaaggtcg tcagcgtggt attgtcaggg ttgttttcga aattggaaat cgtcgcctgc
     2101 ttaataccaa tttttttcgc cagctcgctc tgcgtccagc cattttgctg gcgaaccagt
     2161 ttcattgcat tcgccaattg cgttgggcta tagatcttct gaaagctcat catgtcatac
     2221 gtccacagca aatttatccg cttaagggga tattatcagt tttatccttt agtgaggata
     2281 agtcaatctg gaaacacatc ttatccgctc tacgggataa acactgtgat atccgcgatc
     2341 gcggataaaa aggaggattt aaaagtcgtt tgaagtcggc aatattaagc cgcatgcatc
     2401 ttcgacatgc ggctattccc ttgggtatcg ccttttacgt tcatcttatc ggtgggcgaa
     2461 ttatcgccgc ccagcgtcgc gttgaacgtc atcaggccat tgtttccggt atagttaccg
     2521 ttaacggtca gtacattccc cggtttcccc ccttcgctgt tcatttgaac cgtgccacca
     2581 ttctccaggt taccgccgac agtggtttta gcgacacgca gtgtcccccc tggcataacg
     2641 ttcacatcac ctgcagtggt gacatttccc gacatcacac cgctgttatg gatattaatg
     2701 tgctgacttg ccatattaat ggcagagtcg gaaccgaaag caatttcacc acccacgata
     2761 tccgtcgtac ccttatacgc cgcggaatct ttgttaagcg tcaggatccc ggtacccagt
     2821 tttgtcacat caccgctacc gctgatactg tgcgtcatca cccagctacg ctcagtggag
     2881 atggtgagca ggccattatt gataacggaa gcatcaccca ggtttttctg ctcgttgacg
     2941 ctaagcgcgc tgcctgtatc aatgttgaat tgccccacaa agcggctatt atcgccatcc
     3001 agctctacgt ctgtcctggc ggttgcgctc acgataccct tcccgcttat gctattacgc
     3061 agttcgccgg tcacattttt tagcgttaac agaccgtcat tggcaatatt tcctctgcct
     3121 aacccctgcg tattatcgag gctgacttcc gcatttgggc taatgctggt taacgcattg
     3181 tagcgtgcct taagcccctc gatagccagt gttccccctg taacattcag gtttccgcca
     3241 cctgtcagtt cgccctgact gatcccacct ttattcaccg tcagcgaccc ctctttaaac
     3301 aaaacagtcg aacccatttg cccggtgaat gtctctaccg tctgggtcga tccattaaga
     3361 tcgacgatgg ccgcgttgct gatgttcagt tcccgggtgt tgcccagcgc gccatccgca
     3421 tcggtacgta atgtccccat ctgaacgtag gttgccccct gatagtcgtt ctgaccgttg
     3481 gaaagcgaaa cctgtcgtac cgtattgatt gccagatccc cttcgccacc gatttttgcc
     3541 gacatatcga ccgttgcgcc ataagctccg ccatgttcgg ctaacgtcag cttttgccca
     3601 ccatggatgt tcagcgcttt cagcccatag ttaacataca aaccatcacc aggtgcggtc
     3661 gtgaggcgga atccataatc accctcggcg accaccgttc cgttttgcgc aatatgtaac
     3721 gttttgtccg cttccacctc gtcgccctgt aaatcacgta acgttaatga accacccgat
     3781 ccaataaccg tttgcgcctt caccagttga acgccgacat ggctatcgtc gtgttccatc
     3841 aaattgagcg tcgtcagagg gttattcgcc atgggatcat tccacggttt aggcacgtcg
     3901 ataagcacgt cgcccatccc gtttacctga tagttacggc ctttccaggt gtagtcaccc
     3961 gcgccgacaa ccagcgtatc gacgctgata tatccctccg caagcgtcgc agcaggtata
     4021 tccgtatcga acgtgagcgt accgccattc atggctagtc caccaatgga ttgctctccc
     4081 acttttaccg atgtggtatt ttcactgtca gactgcagca tcgcgtaagt aagcgcagcg
     4141 gtgttgtcgc gttccagtgc gaaggtactg gctttcagtt gggcaacacc ggcgaattca
     4201 gtccctgctg catgggtaaa gccaaacatt ttgtcggagg atgacagctg cactcgcatc
     4261 aagccatcgc ctttgagcgc attgatcagc gtgaatagca atttcccctt gaatatagta
     4321 ctggtgatgg aacgatgaat ctgcaagctg gcatttaaca agccagctct aaaagaaggg
     4381 aaatgagaat accaatactc aaaaactaac agccacggtc atcatggtgt ggctgtcaat
     4441 gaaactataa cccaggcgct ttccataacg acgtcgtcag tccatgatca accagcccca
     4501 gctgatcctg ataaactgcc tgccacttat cgcgtgaatc ctgataaagt tcatgctttt
     4561 ccgggtctgg tgtgtgcgtc cgttcccaac gaaccaagcg ttctccggtt tctgccattg
     4621 atgaaaaaat tccggcaccg acgccagctg caatggcaca tcctaatgca gtggcttctt
     4681 tgaccaccgg aatattgacg ggtaatcccg agacatcagc gagaatttga ctccataatt
     4741 tcccttttga acctccgcca gcaaaaacca gcgaggtggg atgaatattc gagaaatcag
     4801 caatttgctg taagttacac gctgatacaa tcgccgcatt ttcttccagc gcacggaaca
     4861 atgtcgcttt gttacattta tccgggtcaa tggacaagtt aataaaggaa ggcgcagcgt
     4921 gataccaggt tttaaagcgc attctgtcgg agaatatcgg cattacgccc cacgacccag
     4981 gcggcacccg actggccatc tcttccagca gcgtataggt gtcgatgcct aaacgttcgg
     5041 caatcagttt ttcttcggca cagaaagcat cgcggaacca gcgcatggtg agtccggtaa
     5101 aaaagcttat agattcagct tgtaccatgc caggaataac atggggatta acgcgcacgt
     5161 tcatttctgg gtctgtcacc ggcgcggcta agtttacaac ttgctgccag aatgtgccgc
     5221 caagaaccgc ggtttgtgcc ggacgtacaa cgcctaaccc caggcaacca agctgcacgt
     5281 cacctcctcc aacgaccacc ggagtgcccg ccttcagacc gcagagttcc gccgcttgtg
     5341 aacttaccac acccagcaat gtgccggttt ctttgacagg agaaagaata tcggcacgca
     5401 ggccagccat atccagcaat gcaggtttcc agtcacgggt ggttagatca ataagtcccg
     5461 tggtgccagc gttagaggga tccaccgcaa gttcgccgct gagcatatag gccagccagt
     5521 cgctgatcat ggtgatggtt gatgcctgac ggtaaatatc ggaacgatgg tgcgccagcc
     5581 aaagtaatct ggggatggcg cttaaagcca gcgtttgccc ggtcgctcga taaactttgt
     5641 tttcaaaggt attgttgtgc agttctttaa gttcgttaac ttcgcgcgcc gctctggcat
     5701 ccacattggc gcaggcccag atcggtgttc cttcattgtt atataaaaca atgccttcac
     5761 gcatcgaaca tgccgaaaca gcagcgatat actccggggc tatgccggcg ttgtgcagcg
     5821 cctggcgcat acactcacac gccagttgcc agtttttgtt gagatcaaat tccatagaac
     5881 caggaacgtc cggtactgcc agatgccgcc actccgcctg tcccacggct atttgattgc
     5941 cttccgggtc gaatatcaca gcccgaatac ttccggtgcc tgcatccagc gccatcaggt
     6001 agtactttga ttctgaaggg gtaaagagtc gagccattag attatcctcg gcttatgcgt
     6061 tctgtctgtt cctctatacg ttctccatca ttcccggtaa caaggtcatg caaatttaac
     6121 tacgtaaaat cgccgctgct gtgtcctgat cggtaaccag tgcgttgata tagccgcctt
     6181 tcattgcagc ggcaatggct tcggctttat tttctccccc tgccacgcca acccggacgg
     6241 gtatggtctt cagcgcgctt aaaggtaagc caatcagttc gttatgtatt ttaatatccg
     6301 tgacaacgtc accttttgca tcaaaaaagt agcctaaaat gtcgccaaca gccccttttc
     6361 gaccaatcat taactgttcg ccctggctga tatagccgga gcgaatgatt gttgcgtcgt
     6421 cttgttgact cacagcccca atgccgacaa tcgccacatc cgctgcttgc gcggctagca
     6481 gaacatcttt gacgcaattt tcatttttta gcgtacgggc aatgtcagcg gaggatgccc
     6541 gcaacggagc cggaataata ttcacactgc acgccgcgtt aagctgcccg atacccgtca
     6601 tataagaacc gactccaccg gagagcgtga ccaggcgaat ttgctgtgac gaaataaaac
     6661 cacttaagcg ttgcagcgta ttcatagttg cctcgccaaa accaatcgcc agcatctgtt
     6721 gtggttgaag taaactcatc aacatatgcg ccgcgcctat ccctagtcgc ccgccgacat
     6781 cagcatccgc aagccctggg atcacccgga catgttgcag cgaaaactga cgacgtaatt
     6841 gtgtttcata ttccagacag ccttcaaagc gagaattaat ctgtacgcga ataatgccgg
     6901 attgatgccc tttctccagt aatcgcgaca ctttcaaacg ggtcaggccg agacgatcgc
     6961 tgatctcact ctgggtcagc ccgtcgtggt agtaaaacca cgcaatccgc gcgacctgtt
     7021 cttcttcaca cattccctgt tctgaaatta ccgaatcgtt gattgtcata attcattctt
     7081 cactttgaac atatttaaat ctttaatgca attgttcagt tcttgcgcat ttatatctgt
     7141 gatagcaacc acagtttgac tctacgagca tgaacaaacg caaccgtgaa aatcaaaata
     7201 gcataaattg tgatctattc gtcggaaata tgtgcaatgt ccacctaagg ttatgaacaa
     7261 attaaaagca gaaatacatt tgttcaaaac tcacctgcaa aactgaacgg gggaaatatg
     7321 caaacgagtg atacccgcgc gttaccgcta ctttgcgccc gctcggttta taaacagtat
     7381 tcaggggtta atgtcctgaa aggcatcgat tttacgttgc atcaggggga ggtccacgcc
     7441 ctgctcggcg gcaatggtgc cggtaaatcg acgttaatga agattattgc cggtattacc
     7501 cctgctgata gcggtacgct ggagattggg ggcaataatt acaccagatt aacgccagtt
     7561 catgctcatc agctgggcat ttatctcgtt ccccaggaac cgctgctttt tccaagcctg
     7621 tcgataaaag aaaacatcct gtttggtctg gcaaaaaaac aactctccat acagaaaatg
     7681 aataacttgc tggtggcgct gggctgccag tttgatctgc atagtctggc aggatcgctg
     7741 gatgtcgccg atcgccaaat ggtggaaatc ctccgcggcc tgatgcgcga ctcgcggatt
     7801 ctgatcctcg atgaacctac agcctcgctt acccctgcgg aaaccgaacg tttgtttcgt
     7861 cgcttgcaag agctgcttgc tactggcgtg ggtattgttt ttatctcgca taagctgccg
     7921 gaaattcgcc agattgccga tcgaattagc gtgatgcgcg acggaaccat cgccttaagc
     7981 ggcaaaacca gcgaactgtc taccgacgac attattcagg ccatcacgcc agcgctacgg
     8041 gaaaaatcgc tctctgccag ccaaaaatta tggctggaat tacctggcaa ccgcccacaa
     8101 catgccgcgg gaacgccggt gctgacactg gaaaatctga ccggcgaagg tttcaggaat
     8161 gtcagcctga cgctcaatgc cggagaaatt ctgggcttgg ctgggctggt gggagccgga
     8221 cgcacagaac tggccgagac gctctatggt ctgcgtactt tgcgtggcgg acgcattatg
     8281 ctgaatggta aagagatcaa tagattatcc accggagatc gtttactgcg cggtctggtt
     8341 tatctgccgg aagatcgcca gtcatccgga cttaatctcg atgcttcact ggcgtggaac
     8401 gtctgcgccc ttactcataa ccttcgtgga ttctgggcga aaaccgcgaa agataatgcc
     8461 accctggaac gatatcgccg ggcgctgaat attaaattta accaaccgga acaagctgcg
     8521 cggaccttat ccggcggcaa ccagcaaaaa atcctgattg ccaaatgtct ggaagcttcg
     8581 ccgcaagttt tgattgtcga tgagccgacg cgcggcgtgg atgtctcggc gcgtaatgat
     8641 atctaccaac tgttgcgcag catcgccgca caaaatgtgg ctgtgctgct tatctcctct
     8701 gatctggaag agatcgaact gatggcagat cgcgtgtatg tgatgcatca gggcgaaatt
     8761 gcccactctg cactgaccgg acgcgatatt aatgtcgaga ccattatgcg cgttgccttc
     8821 ggagatagtc agcgtcagga ggcgtcatgc tgaaatttat tcagaacaac cgtgaaatca
     8881 cggcactgct ggcggtggtg ctgctgtttg tattacccgg ttttctcgac cgtcagtatt
     8941 taagtgtgca aacgctgacc atggtttata gcagcgcgca aatcctgatc ctgctggcaa
     9001 tgggcgcgac gctggtaatg cttacgcgca atattgatgt ttcagtgggt tcgattaccg
     9061 gaatgtgcgc ggtgctgttg gggatgttac tgaacgcggg gtattcacta cctgttgctt
     9121 gtgtcgcgac tttactgctt ggtttgctcg cgggattttt caacggtgtc ctggtcgcgt
     9181 ggctaaagat ccctgccatt gttgccaccc ttggcacgtt agggttgtac agaggcatca
     9241 tgttgctgtg gactggcggt aaatggattg agggattacc cgccgaactg aaacaactct
     9301 ccgccccgct gctgtttggc gtttccgcga ttggttggtt gacgataatt ctggtggcat
     9361 ttatggcctg gctgctggca aagacggcgt ttggacgcag tttttatgtc acgggcgata
     9421 atttacaggg cgctcgtcaa ctgggcgttc gtacggaagc cattcgcatt gtggcatttt
     9481 cgttgaacgg ctgcatggcg gcactggcgg gaattgtgtt tgcttcgcag attggtttta
     9541 tccccaacca gaccggtacc gtgctggaga tgaaagcaat tgcagcctgc gtgctgggcg
     9601 gcattagttt gctcggcggt tccggcgcga tcatcggtgc ggtactcggc gcatggttcc
     9661 tgacgcagat cgatagtgta ctggtgctgt tgcgcattcc ggcatggtgg aatgatttta
     9721 tcgcgggcat ggttctgctg gcggtgctgg tgtttgatgg acgcctgcgt tgtgcgctgg
     9781 aacgtaatct gcggcggcaa aaatatgccc gctttatgat gccaccgcca tccgttaaac
     9841 ccgcttcgtc aggtaaaaaa cgggaggccg cgtaatgcgt attcgctacg gttgggaact
     9901 ggctcttgcc gcactgctcg ttattgagat tgttgcattt ggtgcaatta acccgcggat
     9961 gttagatctc aatatgttgc tgttcagcac cagtgacttt atctgcattg gcattgtcgc
    10021 cctaccgctg acgatggtga ttgtcagtgg cgggatcgat atttcgtttg gttcgaccat
    10081 cggcctctgc gccattgcat tgggcgtact gtttcaaagt ggtgtgccga tgccgctggc
    10141 gattcttctg accttactgc tcggcgcatt gtgcgggctg atcaacgccg gattaattat
    10201 ctataccaaa gttaacccgc tggtgattac gcttggcacg ctgtatctgt ttgccggaag
    10261 cgccctgctg ctttccggga tggccggagc gacggggtac gaaggtattg gtggattccc
    10321 gatggcgttt acagatttcg ctaacctgga tgtgctgggg ctccccgttc cgctgattat
    10381 cttcctgata tgtctcctcg ttttctggct ctggctgcat aaaacccatg ccggacgtaa
    10441 cgtgttttta attgggcaaa gcccgcgtgt ggcggtttat agcgcgattc cagttaaccg
    10501 caccttgtgt gcgctctatg ccatgacggg gctggcgtct gcggtcgccg ctgtgctgct
    10561 ggtatcgtat tttggttcag cacgttccga tctcggtgcg tcgtttctga tgcccgccat
    10621 caccgccgta gtgctcggcg gtgccaatat ttatggtggt tctggttcca ttatcggcac
    10681 cgccatagcg gttttattag tgggatattt gcaacaaggt ttgcaaatgg caggagtgcc
    10741 aaatcaggtg tccagcgccc tttccggtgc gctacttatc gtcgttgtcg taggtcgttc
    10801 cgttagcctg catcgccagc aaattaaaga gtggctggcg cgtcgggcca ataacccatt
    10861 gccataaagg atatcttcat gacacttcat cgctttaaga aaatcgcctt acttagcgcg
    10921 cttggcattg ccgtaatctc tatgaatgtg caggcggcag agcgtattgc atttattccc
    10981 aaactggttg gcgtgggatt ttttaccagc ggtggcaacg gcgcacaaca agcgggtaaa
    11041 gagttgggcg ttgatgtgac ctacgacggg ccgacagaac ccagtgtttc tggtcaggta
    11101 cagttgatta ataacttcgt caatcagggg tataacgcca ttatcgtttc tgcggtttcg
    11161 cctgatggct tgtgtccggc actgaaacgc gccatgcaac gtggtgtgag agtgctgacc
    11221 tgggactctg atactaaacc ggagtgccgc tcttactaca ttaatcaggg aacgcccgcc
    11281 cagttgggag gtatgttggt ggatatggcg gcgcgtcagg tgaataaaga caaagccaaa
    11341 gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa accagtgggt gaaagaagcg
    11401 aaagcgaaaa tcgccaaaga gcatccaggc tgggaaattg tcactacgca gtttggctat
    11461 aacgatgcca ctaaatcgtt acaaaccgca gaaggaatat taaaagccta tagcgatctc
    11521 gacgccatta tcgcccccga tgccaatgcc ctgcccgctg ccgcacaagc cgcagaaaac
    11581 ctgaaaaatg acaaagttgc gattgtcgga ttcagtacgc caaacgtgat gcgtccatat
    11641 gtggaacgcg gcacggtgaa agaatttggc ctgtgggatg tggttcagca aggcaaaata
    11701 tcagtttatg tcgcggatgc attattgaaa aaaggatcaa tgaaaacggg cgacaagctg
    11761 gatatccagg gcgtaggtca ggtggaagtc tcgccaaaca gcgttcaggg ctatgactac
    11821 gaagctgatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa caaagagaat
    11881 atcggcaaat acgatttctg atgtgcatta cttaaccgga gtacgttatg gcagatttag
    11941 atgatattaa agatggtaaa gattttcgta ccgatcaacc gcaacaaaat atccctttta
    12001 ccctgaaagg ttgcggtgca ctggattggg gaatgcagtc acgcttatcg cggatattta
    12061 atccgaaaac gggtaaaacc gtgatgctgg cctttgatca tggttatttt caggggccga
    12121 ctaccggact tgaacgcatt gatataaata tcgccccgct gtttgaacat gccgatgtat
    12181 taatgtgtac gcgcggcatt ttgcgcagcg tagtcccccc ggcgaccaat aagccggtgg
    12241 tactgcgggc gtcaggtgcg aactctattc tggcggaatt aagtaatgaa gccgtggcgt
    12301 tatcgatgga tgatgccgtg cgcctgaaca gttgcgcggt ggcggcgcag gtttatatcg
    12361 gcagcgaata tgaacatcag tcgatcaaaa atattattca gctggtcgat gccggaatga
    12421 aagcgggaat accgaccatg gccgtgactg gtgtgggcaa agatatggtg cgcgatcagc
    12481 gttatttctc gctcgcgact cgaatcgccg ctgaaatggg ggcacagatt atcaaaactt
    12541 attatgtcga taaaggtttt gaacggattg ttgccggatg tccggtaccc attgttattg
    12601 ctggcggtaa aaagttaccg gagcgcgagg cgcttgaaat gtgctggcag gctatcgatc
    12661 agggcgcttc tggtgtggat atggggcgta atattttcca gtctgaccat ccggtggcga
    12721 tgatgaaagc cgtacaggcg gtggtacacc ataacgaaac ggctgatcgg gcatatgaac
    12781 tctatctgag tgaaaaacag taactgcgga tttaaggaga agaattatgc acgtcacact
    12841 ggttgaaatt aacgttcatg aagataaggt tgacgagttt atcgaagttt ttcgccagaa
    12901 ccacctgggc tctgtacagg aagaaggcaa tttgcgcttc gatgtcttac aggacccgga
    12961 agtgaattcg cgcttttata tctacgaagc ctataaagat gaagacgcag tggcattcca
    13021 taaaaccacg ccccactaca aaacctgtgt cgcgaaactg gaatctttaa tgaccgggcc
    13081 gcgtaaaaaa cgtctgttca atgggttgat gccgtgaggc gaatttatca gttttatcta
    13141 caattggggt aacgcgctga cgggagtaaa taaatgtctg actggaaccc ctctttatat
    13201 ctgcactttg ccgctgaacg atcgcgtccg gcggtggagc tgcttgccag agtgccgctg
    13261 gagaatatca aatacgttgc cgatcttggc tgtggcccag gtaacagcac caccctgctg
    13321 catcaacgtt ggcctgcggc caggataaca ggcatcgact cgtctccggc gatgattgct
    13381 gaagcgcgca gtactttgcc agactgccag ttcgtggaag cagacatccg caactggcaa
    13441 ccggaacagg cactcgatct gatttttgct aatgcctcac tgcaatggct gcccgaccac
    13501 tacgaattgt tccctcatct ggtttcttta cttaattcgc atggtgtgtt ggcagtacag
    13561 atgccagata actggctaga gccaacccat gtgctcatgc gcgaagtcgc ctgggaacaa
    13621 aattatccgg atcgcgggcg tgagccgctg gctggcgttc atgcttacta cgatattctg
    13681 agtgaagccg gatgtgaggt cgatatctgg cgaaccactt actatcacca gatgccgtcg
    13741 caccagtcga ttatcgattg ggtgaccgcc actggattac gtccgtggtt acaggatctg
    13801 accgagagcg aacagcagca ttttcttacg cgctaccatc agatgctgga agagcaatat
    13861 cccctccagg agaacggaca gatattgctg gcatttccgc gtctgtttat tgttgcccgc
    13921 cgtacggagt aattatcacg tcagctggta atggcgatcg ggaagaattt tcgctggaat
    13981 ttctgcttca tcgttcatct gtaacaggtc aatttcaata gcgttgcaga tggcatccag
    14041 cggtaagtca ttgttttcag taccgaaggg atcttccagt tcttccgcca gacaatccag
    14101 cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc gtcatgtaat gcaggtccac
    14161 gaccagcgca aacggcagca tgatacaaaa cagatacacg gtgcgatgca aaatcagggt
    14221 gtaggcaaag ggaattggcg tataggcaat gcgctcgcat cctgccagga ccactgaaat
    14281 atcattaagc cgatcgttga ggctaataaa caggatatct gaaagttgtc cattgcgtcg
    14341 ctgaaccgcc aaccactccc ccattattaa caagatgcgg ttagctggag agttagaagc
    14401 cagtacacgc tgaagatctt cagtcttgag ataatgagcc agcacttccg cctgtggctg
    14461 tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt tgcagccggg caaactccct
    14521 tacacttgcc gaatccggca atgtcgtttt tacctcgcgc agtaacgacc gcgaggcaat
    14581 catcaactgc ccccaaagtt ttcgcgcttc aacgtaacga gcgtaaccgg cattattacg
    14641 aaaaccaaga aaaatggcga tggcgacacc gagaatgctg aacggtgcga gggtgaattt
    14701 aatgcccaaa tgcgtgtacc agggcagcat gaaaataaca gcgatagaaa agagaaaatt
    14761 gagtagtaag cgcgaggata tcttggataa tactgaaccg tgccagacaa aaatacggcg
    14821 cagccagtgt tgttgtggac gaacaatcat ggttatcttc aggcgtggaa aagtcgccct
    14881 attaaacgtg attacgatca cattctcaag acactactta caaattacct acaagcttac
    14941 aacattaaca ggtgctgcat gtggcacctg ttaatgataa aggcgcatag gatgttgtaa
    15001 ctaatatagt gaaataagaa acccggtcga aaccgggttc agagtattag cacaacggac
    15061 gtacagcttc gcgcattcct tttttgagaa tcgcatccag gtcattagcc acctgctcga
    15121 ccagaccagg cacttgcgtc aggtcctgct cccagtggtc tttttctgcc aacacaatcg
    15181 ctaccagttc ctgggtaccg ataacacggt cacgatgctg gctccagagt tgctgataac
    15241 gttccagcca gtgtgcatcg tcctgtaccg gatatgtttc tccattacgc tcaccgcgat
    15301 aaaacgcaat caatgccgct aatgcgaaag tcaggcgcgc cggaagtgtg ccgtttgcct
    15361 tctgccctgc cagcagctgc ggcaggatac gggtgcggaa cttggtcata ccgttaagcg
    15421 cgatagacag cagctgatgc ttgatgtacg gattacggaa acgtccggtg actgcactgg
    15481 cgaaagattc cagttcatca cgaggcaaat ccagtaccgg aataatctct tcgtagatgg
    15541 ctttttcaac gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg gtatccagtc
    15601 ctgcctggaa cgccaccggc accagcccgg tgtgcgcacc gttgaggatt gccactttgc
    15661 gctctttata cggtttaata tcgtcaacaa tcagcacgtt gagtggatat ttgtccaggc
    15721 gcagctcagt cgctaaggat ttcggcccct gaatcacaaa caggtaaaag tgttcagcgg
    15781 tgtcgaggaa accatcgtga taacccagtt cttcttccag tttagccact tcatcgcgcg
    15841 gataaccggt aacgatacgg tccaccagcg tagaacagaa gctgttagcc tgatccagcc
    15901 attgaataaa tgcttctggc agtgcccact cttgcgcata gcgcagaacc agttcacgca
    15961 acgcgtcgcc attgtagtcg atcaactcac acgggatgat gatccaacct ttatccagcg
    16021 caccgttgaa atggctaaaa cgttcgaaca gcagacgggt cagttttgcc ggataactta
    16081 ccgctggcgc gtcatcgaat ttatcgcccg catggtagct gatacccgct tcggtggtgt
    16141 tagagaaaac aaagcgcatt tccgggttgt gcgccagttt caggaattca tcgtattcac
    16201 tgtagacgct gatttcacga ttaacagagc gaatcagacg cgcgtcgctg accgcttccc
    16261 ccttctcatt caggccacgg ataatggtgg tgtacagacc atcctgtgtg ctcagtgacg
    16321 gtgggaatga agtttcaatc ggacgaacaa cgaccacgcc agaattcaga tcggtgtgct
    16381 cattcaggag atcaatttgc cagtcaacaa aggcgcgcag gaagttacct tcaccaaact
    16441 gaatgatacg ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt gttttcacaa
    16501 tgggttccct tctgattagt catacaacct gttttaattg gtacgacagg ttagcaaact
    16561 ttaatacgcc gaacccctgt tttgatcaac tcctgatgat taatgagcag ttttatgaga
    16621 aaagtgtggc gcggatcatg gtttaatcga ggaactacgc cttttattcg ctcaataaag
    16681 tggtagaaca cattccgtta aaatcgctgt gaatgaataa agatgtcaga caacttcctc
    16741 accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat acaaacatgt
    16801 atcggcttcg accatcaatt tattaaagtc atcggtcagc gttcggtagg atgcgcaacc
    16861 actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg tgaacggttg
    16921 cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag gattcaccga
    16981 tggcactgca acagcaaatt cttcgccgcc cattcgcgcc accagcccct tatcaccgac
    17041 aatcttctga atatgctggg caaacacgct taacacttta tcgccacatt catgtccata
    17101 gttatcgtta atacttttga aatagtcgat atcaagcagc atgacagtca gatgttgtgt
    17161 ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt aaacctgagt
    17221 cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcgaattga tcgctgccac
    17281 gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac gtgcggagaa
    17341 catttgcgga atggagaacg gcgaaccgac cgagatatca atcaccgaat ttgccaccag
    17401 tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg gcgtatagcg
    17461 cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc cgccaatgac
    17521 taccgaggca ataaccgaca caataagcgc gatagcgggc atcatctgtt ctgctttaaa
    17581 gcgcggcagc actccaggaa ttgccaacgt cagcatacaa ggcacgatca acacgcccgt
    17641 tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac tatcaatact
    17701 gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac agtaattaaa
    17761 tagccgtaag gcacttactg gctcatactt attttttccc agacgcttat cccgcgcgac
    17821 cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgatagcca gtgaaaccag
    17881 cccccattcg gtggttatgg catcataaac cagcatcgcc acataactga tcgcatagta
    17941 atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc cattcagcgg
    18001 ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat agaacagcgt
    18061 ggttagcaca aaaatagcga tgctattacg tagcagatga ccttcctgga acaaacggaa
    18121 agttgaaatg ggctgtacat gcattaatta tctgtccata caattgcggg tggcaattat
    18181 ctcaacgcaa taagtaaatt cttagaaagg atagcatgtt cgctgacaac ggcggcaact
    18241 aaagatatcc tgggaatgct attttatcga tttccgtaat tatcatggct gattcatctg
    18301 tataaccagc cagtggtaga ttaccgtcac aatataacgt tgttgctcct cacttaacgc
    18361 ttccccctga ggaatgttat gccacttagc cagacaaccg cgacaacagg tcgcggtggc
    18421 gtgttgagcg ataaaaaccg gatgcccgcg catgggggtt tgcttaccgt cgttagccgg
    18481 taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcaa tgactggcgc
    18541 gcctttctcc agacaatact gacgctcttt cacacccaaa cgaaagcgag agcgaaattt
    18601 tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac tgaacgccct
    18661 aattgtttcg tcaattgttc aaggacggca atgcccgcaa gcgagttacc ggcatcatcc
    18721 agttccggac tccagactgc gatggccatt tcatgcggaa caatcgccac aataccgcca
    18781 ccaacgccag attttgccgg tagtcccacc cgccaggcaa actcccccgc gttctggtac
    18841 ataccgctgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt caccacaggt
    18901 tcatcaatat gaatagcttt cccctgattc gccagaaaga caaacgtccg ggccagctct
    18961 atacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt tgtcacgtca
    19021 tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg cgcggaatgt
    19081 tcaaattcgg aacgcgctac caccgtatcg taggaaatat cagacacacc acttaagccg
    19141 cgcacgactt ccagcatacg ttgccgaggt gcgcttaatc gcccttgcaa catatcgcag
    19201 accaccagcg caccggcatt aataaacgga ttacgcggaa taccctgctc catttccagt
    19261 tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg ccagatttcc
    19321 tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat agactgaatg
    19381 gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt acagatagca
    19441 atccccattc gggaaccgtc taccgtagcc agcgccggaa tataatccgc gactttaccc
    19501 tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt atccatggcg
    19561 actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat atcagagctg
    19621 aatatgtagc gtcagatccg gtctttccac accgtctgga tattacagaa ttcgtgtaag
    19681 ccaaaatggg aaagctcacg cccaaagcca ctcttcttca cgccaccaaa agcgactcgc
    19741 gcgtcgctgg cacagtaacc attgataaac accccaccgc attccagacg tgccgccatc
    19801 tgtctggccc gggtttcgtc ggtggtgaaa atggtcgctg aaaggccgaa ctcgctatca
    19861 ttagccagtt ccagtgcatg ttctgcatct ttcgcaatgg tgattgccgc aacagggcca
    19921 aacatttctt cccgaaacgc ggtcatttct ggggtaacgt tcgccagaac cgttggcgga
    19981 tagtagttac ctgccccagc catcttttcc ccgcccagta acaaacgcgc accctgcgcc
    20041 agggtttttt ccacctgatg atgcagctca tcacgtaaat caaaacgagc cattggtccg
    20101 agagcgttct cttcgtcacg gggatcgccc attttcaagg ctgccgcagc tgccacaaaa
    20161 cgttcggtaa atgccgaagc aattccctct tcgataataa agcgttttgc cgccgcacaa
    20221 acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag ttccagatcg
    20281 gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca ttttttcagt
    20341 gccgctccag cctgtgcgcc aatagccgct cccgcacgaa cacttccggt cactgtgaca
    20401 gcagcaatgc gcgaatcttt aatcatctga ctgacaccgt tgttgtcagc attcagccag
    20461 ccatatacgc cttgcgggat acccgcatct ttaaacacct gggcaatgag ctgtgcacag
    20521 cccatcacat tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaacc
    20581 gcgccacgca tcacctgcca taacggaaaa ttccacggca taatcgccag aattgtcccc
    20641 aacggtcgat actcaataac tgcctgctga ttttccacca gcgtaggttc cgccttcagc
    20701 attgccggac catgttctgc ataccagtca cacaaattcg ccgatttcgc cacttcagcg
    20761 cgcgcctggt tgattggttt gcccatttca cgggtgatca tttgcgccat ttcttcgcta
    20821 cggacacgca gagccttacc gatatcacgc agtttttcag cacgataatc tatatttgtc
    20881 tcgcgccagt cgcgaaagcc tgctgccgcc agctgaagtg cgttttcgat atcatcagcg
    20941 ccagcccacg gcagcacaga aagttgttca ccggtggcag gatttatcga aattgcatga
    21001 gttgccggag taatggtcat cggggtatct cctttatgag ttatggtaaa aagatacgca
    21061 gatttactct tgctttaaaa tgaataatat taagccactt attcacgaat cgagaatatt
    21121 atggatctga cccaactgga gatgttcaac gcggttgccg aagctggcag cattacgcag
    21181 gctgcaacaa aagtgcatcg cgtgccgtcg aatctcacta cccgtctacg ccagctggaa
    21241 acagaactgg gggttgatct gtttattcgc gagaatcagc gtttacgtct ctcgcctgcc
    21301 gggcataact ttttacgcta tagccaacaa attctcgcgt tagtggatga agcgcggagc
    21361 gttgtcgctg gcgatgagcc gcaaggttta ttttctcttg gttcgctgga aagcaccgca
    21421 gcagtgcgca ttccggccat gctggcggaa tttaaccgtc gttatcccaa aattcagttt
    21481 tcactttcca ccggcccttc cggcactatg ctggaagggg tgctggaggg aaaactgaat
    21541 gcggcgttta ttgatgggcc cattaaccat actgccattg acgggatacc ggtgtaccgc
    21601 gaggagctga tgattgtcac gccacaagga catgcgccag taacccgtgc cagtcaggtt
    21661 aatggcagta acatttatgc cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc
    21721 tggtttcatg ctgacggtgc cgctccggga actattcacg agatggagtc gtatcacggg
    21781 atgttggcct gtgtggtcgc aggagcaggc attgcgctta ttccacgctc aatgctggaa
    21841 agtatgccgg ggcatcacca ggttgaagcg tggccgttag ctgagcaatg gcgttggtta
    21901 acaacctggc tggtctggcg tcgcggtgcg aaaacgcgtc cgcttgaggc atttattcaa
    21961 ctactggatg tgtctgactc ggcaaaacag agttagtaat gagctatttt tgatagttct
    22021 tgcggctaat atgctctata tagtgatgct ccgatgactt atgactatat ggggcaaata
    22081 tggttacgcc agtaagcatc agcaatttca tctcacttcc tgatgatttt cccgttcgta
    22141 atattgcacc tcaggtaaaa gaagaacagg cccccttcct gatcgtcatt aaaatgtgat
    22201 gttaacaact ctctgatgcg tgacagaaca accgtcagag agatggaaat gcgatccaga
    22261 tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc actttttcgc
    22321 aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg gcgtggctac
    22381 gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt gtcccagttg
    22441 gcctgctctc tgacattgcg cagagttttc acatgcaaac cgctcaggtc ggcattatgt
    22501 tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta atgaccagcc
    22561 aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc agccacgtac
    22621 tgtcgttttt atcgtggagt tttaccgttc tggtgatcag tcgcattggt gtggcttttg
    22681 cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct ccggccggga
    22741 agcgagcaca ggcattgagt ttaattgcca ccggtactgc actggcgatg gtcttaggtt
    22801 tacctctcgg gcgcattgtg gggcagtatt tcggttggcg aatgaccttc ttcgcgattg
    22861 gtatcgggga gcttatcacc cttttgtgcc tgattaagtt acttccctta ctgcccagtg
    22921 agcattccgg ttcgctgaaa agcctcccgc tattattccg ccgcccagca ttgatgagca
    22981 tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc tatatcgagc
    23041 cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta ctgttattac
    23101 tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat cagtatgcgt
    23161 ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg cctggcactg ctgctacctg
    23221 cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggata gcgatgatga
    23281 tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgct accgacgtcg
    23341 cgatggcgct attctccggg atatttaata ttggaatcgg cgcgggtgcg ttggtaggta
    23401 atcaggtaag tctgcactgg tcaatgtcga tgattggtta tgtgggcgcg gtgcctgcct
    23461 ttgccgcgtt aatatggtca atcatcattt tccgccgctg gccagtgaca ctcgaagaac
    23521 agacgcaata gttgaaaggc ccatttgggc cttttttagt ggtacgtttt aatgatttcc
    23581 aggatgccgt taataataaa ctgcaccccc atacatacca gcaggaatcc catcagacgg
    23641 gagatcgctt caatgccccc cttacccacc agccgcataa ttgcgccaga gctgcgtaga
    23701 cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac catcagtacc
    23761 caatcagcaa aggttgaact ctgacgcact gtggacgccg agctaataat catcgctatg
    23821 gttcccggac cggcggtact tggcattgcc agtggtacaa aagcaatatt ggcgctgggt
    23881 tcatcttcca gctcttccga cttgcttttc gcttctggtg aatcaatcgc tttctgttgc
    23941 ggaaagagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat tcgcagaccg
    24001 ggaatcgaaa tgccaaatgt atccatcacc agttgcccgg cgtaatacgc caccatcatg
    24061 atggcaaaga cgtacaccga ggccatcaac gactgacgat tacgttcggc actgttcatg
    24121 ttgcctgcca ggccaagaaa taacgcgaca gttgttaatg ggttagctaa cggtagtaac
    24181 accaccagcc ccaggccaat tgctttaaat aaatctaaca ttggtggttg ttatcctgtg
    24241 tatctggttt atcagcgaaa agtataaggg gtaaacaacg ataaagtgtc actctttagc
    24301 taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg catcggtcaa
    24361 ttcattcatt tgacttatac ttgcctgggc aatattatcc cttgcaacta attacttgcc
    24421 agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc attgggtcgc
    24481 ttaatccata tggttaatca gaagaaagat cgcctgctta acgagtatct gtctccgctg
    24541 gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc gtgtattact
    24601 ccggttgaac tgaaaaaggt attgtcggtc gacctggggg cgctgacccg tatgctggat
    24661 cgcctggtct gtaaaggctg ggtggaaagg ttgccgaacc cgaatgacaa gcgcggcgta
    24721 ctggtaaaac ttaccaccag cggcgcggca atatgtgaac aatgccatca attagttggc
    24781 caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagtggc aacacttgag
    24841 catttgctta agaaagtcct gccgtaaaca aaaaaagagg tatgacgatg tccagacgca
    24901 atactgacgc tattaccatt catagcattt tggactggat cgaggacaac ctggaatcgc
    24961 cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac ctgcaacgga
    25021 tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt aagatgacgg
    25081 aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca gaacgatatg
    25141 gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat gttccgccac
    25201 ataaataccg gatgaccaat atgcagggtg aatcgcgctt tttacatcca ttaaatcatt
    25261 acaacaacta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc acttttatcc
    25321 gcaatagcaa ctgcgcttat tctcttttct gcgcagggcg ttgcggaaca aaccacgcag
    25381 ccggttgtta cttcctgtgg caatgtcgtg gttgttccca catcgcagga acaaccaccg
    25441 tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg cgtgccctat
    25501 tataatcaac acgctatgta gtttgttctg gccccgacat atcggggctt attaacttcc
    25561 cacttttacc gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat
    25621 gagcaccgca ccgaaaaatt gtagacccgt taagcgttca tccaataata gtgccgcact
    25681 tgccagtcct actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg
    25741 tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat
    25801 cagagacaag atggtggtca tatcgatagt aaccagactg tgaatcatgg ttgcggaacc
    25861 atcgagaatc agcgaggcaa caaagaaggg aatgattggg attaatgcgc tccagattac
    25921 cagcgacatc accgccgggc gcgttgagtg cgacatgatc tttttattga agatgttgcc
    25981 acacgcccaa ctaaatgctg ccgccagggt caacataaag ccgagcatcg ccacatgctg
    26041 accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc
    26101 caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa
    26161 cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt taatggcgca
    26221 aaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccagca gcaaattcag
    26281 tggtactttc ggtcgagcga caaaaaagat ggccggaaaa gcgaccagca taaagcgcaa
    26341 accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca caaaattcag
    26401 cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca ttataccgcc
    26461 tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagtcg
    26521 attcgttagt caccgtttac gacggcatta atgcgcaaat aagtcgctat acttcggatt
    26581 tttgccatgc tatttcttta catctctaaa acaaaacata acgaaacgca ctgccggaca
    26641 gacaaatgaa cttatcccta cgacgctcta ccagcgccct tcttgcctcg tcgttgttat
    26701 taaccattgg acgcggcgct acgctgccgt ttatgaccat ttacttgagt cgccagtaca
    26761 acctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt
    26821 ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg
    26881 caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac gtgacgctgg
    26941 tggtgctctt ttttgccctc attaactgcg cctattccgt ttttgctacc gtgttgaaag
    27001 cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca
    27061 ccatgctcaa cattggctgg accatcggtc cgccgctcgg cacgttgttg gtgatgcaga
    27121 gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg cttttcattc
    27181 aaatttgggt aaagcgcaac gagaaaatca tcgccacgga aacaggcagt gcctggtcgc
    27241 cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt
    27301 cctttgttag cggcgcattt gcctcgtgca tttcacaata tgtgatggtg attgctgatg
    27361 gtgattttgc cgaaaaggtg gttgcggttg ttcttccggt taatgctgcc atggtagtta
    27421 cgttgcaata ttccgtgggc cgccgactta acccggctaa catccgcgcg ctgatgacag
    27481 caggcaccct ctgtttcgtc attgggctgg tcggttttat tttttccggc aacagcctgc
    27541 tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg
    27601 agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgctc
    27661 agtctttagg ctggcttggt gcggcgatta acccattagt gagtggcata gtgcttacca
    27721 gtctgccgcc tttctcgctg tttatcatct tagcgttggt gatcattgct gcgtgggtgc
    27781 tgatgttaaa aggaattcgt gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt
    27841 cgaacacaat aaaaatttaa ttcagccttc gtttaggtta cctctgctaa tatctttctc
    27901 attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa
    27961 tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc
    28021 tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa
    28081 ccggcccctt gttacaactg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc
    28141 gccagtaaac cggcggtgaa tgcttgcatg gatagatttg tgttttgctt ttacgctaac
    28201 aggcattttc ctgcactgat aacggatcgt tgacacagta gcatcagttt tctcaatgaa
    28261 tgttaaacgg agcttaaact cggttaatca cgttttgttc gtcaataaac atacagcgat
    28321 ttcttccggt ttgcttaccc tcatacattg cccggtccgc tcttccaatg accacatcca
    28381 gaggctcttc aggaaatgcg cgactcacac ctgctgtcac ggtaatgttg atatgccctt
    28441 cagaatgtgt gatggcatgg ttatcgacta actggcaaat tctgacacct gcacgacatg
    28501 cttcttcatc attagccgct ttgacaataa tgataaattc ttcgccaccg tagcgataaa
    28561 ccgtttcgta atcgcgcgtc caactggcta agtaagttgc cagggtgcgt aatactacat
    28621 cgccgattaa atgcccgtag gtatcattaa ccaatttaaa tcggtcaata tccaacaaca
    28681 ttaaataaag attcagaggc tcagcgttgc gtaactgatg atcaaaggat tcatcaagaa
    28741 cccgacgacc cggcaatccc gtcaaaacat ccatattgct acggatcgtc agcaaataaa
    28801 ttttgtaatc ggttaatgcc gcagtaaaag aaagcaaccc ttcctgaaag gcgtcgaaat
    28861 gcgcgtcctg ccagtgattt tcaacaatag ccagcattaa ttcccgacca cagttatgca
    28921 tatgttgatg ggcagaatcc attagccgaa cgtaaggtaa ttcatcgtta tcgagtggtc
    28981 ccagatgatc aatccaccga ccaaactggc acagttcata agaatggtta tccgttattt
    29041 ctggcttact ggcatctctc gcgaccacgc tgtgaaacat acttaccagc cactggtagt
    29101 gggcatcgat agccttattg agatttaaca agatggcatc aatttccgtt gtcttcttga
    29161 tcattgccac tcctttttca cagttccttg tgcgcgctat tctaacgaga gaaaagcaaa
    29221 attacgtcaa tattttcata gaaatccgaa gttatgagtc atctctgaga taacattgtg
    29281 atttaaaaca aaatcagcgg ataaaaaagt g
//
LOCUS       DAZGAP010000043        29182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGAP010000043 DAZGAP010000000
VERSION     DAZGAP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.043"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..1139
                     /gene="wza"
                     /locus_tag="ACHLAM_001593"
     CDS             3..1139
                     /gene="wza"
                     /locus_tag="ACHLAM_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001746257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO7853143.1"
                     /translation="MKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFD
                     LDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPEL
                     TTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQV
                     DVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNG
                     REERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMT
                     LTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEFR
                     LQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1145..1588
                     /gene="wzb"
                     /locus_tag="ACHLAM_001594"
     CDS             1145..1588
                     /gene="wzb"
                     /locus_tag="ACHLAM_001594"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO7853144.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFVAVYTLLERSARQWAQALNAEQV"
     gene            1591..3753
                     /gene="wzc"
                     /locus_tag="ACHLAM_001595"
     CDS             1591..3753
                     /gene="wzc"
                     /locus_tag="ACHLAM_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO7853145.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGEITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            3931..4770
                     /gene="wcaA"
                     /locus_tag="ACHLAM_001596"
     CDS             3931..4770
                     /gene="wcaA"
                     /locus_tag="ACHLAM_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO7853146.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            4773..5261
                     /gene="wcaB"
                     /locus_tag="ACHLAM_001597"
     CDS             4773..5261
                     /gene="wcaB"
                     /locus_tag="ACHLAM_001597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO7853147.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5258..6475
                     /gene="wcaC"
                     /locus_tag="ACHLAM_001598"
     CDS             5258..6475
                     /gene="wcaC"
                     /locus_tag="ACHLAM_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO7853148.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQRVAGKRQLFSEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQSSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6450..7667
                     /gene="wcaD"
                     /locus_tag="ACHLAM_001599"
     CDS             6450..7667
                     /gene="wcaD"
                     /locus_tag="ACHLAM_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO7853149.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7678..8424
                     /gene="wcaE"
                     /locus_tag="ACHLAM_001600"
     CDS             7678..8424
                     /gene="wcaE"
                     /locus_tag="ACHLAM_001600"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO7853150.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAHGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8440..8988
                     /gene="wcaF"
                     /locus_tag="ACHLAM_001601"
     CDS             8440..8988
                     /gene="wcaF"
                     /locus_tag="ACHLAM_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO7853151.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9015..10136
                     /gene="gmd"
                     /locus_tag="ACHLAM_001602"
     CDS             9015..10136
                     /gene="gmd"
                     /locus_tag="ACHLAM_001602"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO7853152.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYDMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10139..11104
                     /gene="fcl"
                     /locus_tag="ACHLAM_001603"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10139..11104
                     /gene="fcl"
                     /locus_tag="ACHLAM_001603"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO7853153.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11107..11586
                     /gene="gmm"
                     /locus_tag="ACHLAM_001604"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11107..11586
                     /gene="gmm"
                     /locus_tag="ACHLAM_001604"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO7853154.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11583..12806
                     /gene="wcaI"
                     /locus_tag="ACHLAM_001605"
     CDS             11583..12806
                     /gene="wcaI"
                     /locus_tag="ACHLAM_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO7853155.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWQPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            12809..14245
                     /gene="cpsB"
                     /locus_tag="ACHLAM_001606"
     CDS             12809..14245
                     /gene="cpsB"
                     /locus_tag="ACHLAM_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO7853156.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAIDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14438..15808
                     /gene="cpsG"
                     /locus_tag="ACHLAM_001607"
                     /gene_synonym="manB"
     CDS             14438..15808
                     /gene="cpsG"
                     /locus_tag="ACHLAM_001607"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO7853157.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     NNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            15863..17257
                     /gene="wcaJ"
                     /locus_tag="ACHLAM_001608"
     CDS             15863..17257
                     /gene="wcaJ"
                     /locus_tag="ACHLAM_001608"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO7853158.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17259..18737
                     /gene="wzxC"
                     /locus_tag="ACHLAM_001609"
     CDS             17259..18737
                     /gene="wzxC"
                     /locus_tag="ACHLAM_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO7853159.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLIVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19398..20678
                     /gene="wcaK"
                     /locus_tag="ACHLAM_001610"
     CDS             19398..20678
                     /gene="wcaK"
                     /locus_tag="ACHLAM_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO7853160.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20675..21895
                     /gene="wcaL"
                     /locus_tag="ACHLAM_001611"
     CDS             20675..21895
                     /gene="wcaL"
                     /locus_tag="ACHLAM_001611"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO7853161.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLCGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21906..23300
                     /gene="wcaM"
                     /locus_tag="ACHLAM_001612"
     CDS             21906..23300
                     /gene="wcaM"
                     /locus_tag="ACHLAM_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO7853162.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23458..24453
                     /locus_tag="ACHLAM_001613"
     CDS             23458..24453
                     /locus_tag="ACHLAM_001613"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO7853163.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLTAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24696..25589
                     /gene="galF"
                     /locus_tag="ACHLAM_001614"
     CDS             24696..25589
                     /gene="galF"
                     /locus_tag="ACHLAM_001614"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO7853164.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            25961..27046
                     /gene="rfbB"
                     /locus_tag="ACHLAM_001615"
     CDS             25961..27046
                     /gene="rfbB"
                     /locus_tag="ACHLAM_001615"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO7853165.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            27046..27945
                     /gene="rfbD"
                     /locus_tag="ACHLAM_001616"
     CDS             27046..27945
                     /gene="rfbD"
                     /locus_tag="ACHLAM_001616"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO7853166.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            28003..28881
                     /gene="rfbA"
                     /locus_tag="ACHLAM_001617"
     CDS             28003..28881
                     /gene="rfbA"
                     /locus_tag="ACHLAM_001617"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO7853167.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFHQLLGDGGQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLRMIKGY"
     gene            28886..>29182
                     /locus_tag="ACHLAM_001618"
     CDS             28886..>29182
                     /locus_tag="ACHLAM_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFO7853168.1"
                     /translation="MNVIKTEIPEVLIFEPKKFEDSRGYFFESFNQRLFEDAVGKEIL
                     FVQDNQSYSSKNVLRGLHYQSEPYAQGKLVRCIMGEVFDVAVDIRKESESYGQWV"
BASE COUNT         7290 a         7067 c         7852 g         6973 t
ORIGIN      
        1 tgatgaaatc caaaatgaaa ttgatgccat tattggtgtc agtaaccttg ataagcggtt
       61 gcacagtact tccgggcagc aatatgtcga cgatgggtaa agacgtcatc aaacagcagg
      121 acgctgattt cgatctcgac aaaatggtga atgtttatcc gttgaccccg cgcctgattg
      181 atcaattacg cccacgcccg aatgtagcgc gccccaatat gacgctggaa agtgagatcg
      241 cgaattatca gtatcgcgtc gggccggggg acgttcttaa tgtcaccgtc tgggatcacc
      301 cggaactcac cacgccagcc ggtcagtacc gcagctccag cgacaccggc aactgggtac
      361 agcctgacgg caccatgttc tacccgtata tcggcaaggt tcacgtcgtg gggaaaacgc
      421 tcgctgaaat ccgcagtgat attaccggtc gcttagcgac gtacatcgct gacccgcagg
      481 tggacgttaa tatcgccgcc ttccgctcgc aaaaagccta tatctcaggt caggtgaata
      541 aatccggtca acaggcgatt accaacgtac cactgaccat tctcgatgcc atcaacgccg
      601 caggtggcct gaccgacacc gctgactggc gcaacgtggt gctaacacac aatggtcgtg
      661 aagaacgcat ttctttgcag gcgctgatgc aaaacggcga cctcaatcag aaccgactgc
      721 tttaccccgg cgatattctc tacgtgccgc gtaatgatga tctgaaagtg tttgtgatgg
      781 gtgaagttaa aaaacagagc accctgaaaa tggactttag cggcatgacc ctaactgaag
      841 ccctgggtaa tgctgaaggc atcgacatga ccacctccaa cgccagcggc atctttgtca
      901 ttcgtccgtt gaaaggcgag ggcgggcgta acggcaagat tgccaatatc taccagctgg
      961 atatgtccga tgccacatcg ctggtgatgg cgacagaatt ccgcctgcaa ccttatgacg
     1021 tggtgtatgt caccaccgcc ccggtttccc gctggaaccg tctgatcaat cagttgctgc
     1081 caactattag cggtgtccgt tacatgacgg atacagccag cgacattcat aactggtaat
     1141 cgtcatgttt aacaacatct tagttgtctg tgtcggcaat atttgccgtt ccccgacggc
     1201 ggaacgctta ctgcaacgtt atcacccgga gctgaaagtg gagtccgctg gactcggcgc
     1261 gctggtcggt aagggcgctg accctactgc tatcagcgtc gccgcagaac atcaactgtc
     1321 tctggaaggt cactgtgcac ggcaaatcag ccgtcgcctg tgtcgcaact acgacctgat
     1381 tttgaccatg gaaaagcgcc atatcgaacg cttatgcgag atggcaccgg agatgcgcgg
     1441 caaagtgatg ctgtttggtc actgggataa cgaatgtgaa atccccgatc cgtatcgcaa
     1501 aagccgggaa acgtttgtag cggtgtacac attacttgaa cggtctgccc gccagtgggc
     1561 gcaggcattg aacgcagagc aggtataaga atgacagaaa aagtaaaaca acatgccgct
     1621 ccggtaacgg gcagtgatga aatcgatatt ggtcgcctgg tcggcaccgt cattgaagcg
     1681 cgctggtggg tgattggcat caccgccgta tttgccctct gtgccgtggt ttacaccttc
     1741 ttcgctacgc cgatatacag cgccgacgca ctggtacaaa tcgaacaaag cagcggcaat
     1801 tcgttagtgc aggatatcgg atcggcatta gccaacaaac cgcctgcatc ggacgccgag
     1861 atccagttga ttcgttcgcg cctggtgctt ggtaaaacgg tggacgatct cgacctcgat
     1921 attgcggtga gcaaaaacac gttcccgatt ttcggtgcgg gctgggatcg cctgatggga
     1981 cgccagaacg agacggtaaa agtgaccacc tttaaccgtc ctaaggagat ggcggatcag
     2041 gtgtttacgc ttaatgtgct tgatgacaaa aactacactt tgagcagcga tggcggcttt
     2101 agcgcccgtg ggcaagcggg ccagatgctg aaaaaagaag gcgtcacgct gatggttgaa
     2161 gctattcacg ccagcccggg cagtgagttt accgtcacca aatactccac gctggggatg
     2221 atcaatcaac tgcaaaacag cctgacggta acggagaacg gcaaagatgc tggcgttttg
     2281 agcctgactt atactggaga agatcgcgaa cagatccgcg acattcttaa cagtatcgcc
     2341 cgtaactatc aggaacaaaa tattgagcgc aaatcggcgg aagcgtcgaa aagcctcgct
     2401 ttcctcgcgc aacagttacc ggaagtacgt agccgcctcg atgttgccga aaacaaactg
     2461 aatgccttcc gtcaggataa agattcagtt gatctgccgc tggaagcgaa agcggtgctc
     2521 gattcgatgg tgaacatcga cgcgcagttg aacgaactga cctttaaaga agcggaaatc
     2581 tccaagctgt acaccaaagt tcacccggcg tatcgcacgc tgctggagaa acggcaggcg
     2641 ctggaagacg aaaaagccaa acttaatggt cgcgtaacgg cgatgccgaa aacccagcag
     2701 gaaattgtcc gtctgacccg cgatgtcgag tctgggcagc aggtctatat gcaactgctg
     2761 aataaagagc aggagctgaa aatcaccgaa gccagcaccg tcggcgatgt gcgcattgtt
     2821 gacccggcaa tcacccagcc tggtgtgcta aaaccgaaga aagggctgat tatccttggg
     2881 gcgattatcc ttggcctgat gctctctatc gtgggtgtgc tgctgcgctc gttgtttaat
     2941 cgcggtatcg aaagtccgca ggtgctggaa gaacacggta tcagcgtcta tgccagcatc
     3001 ccgctgtcgg agtggcagaa agcgcgcgat agcgtcaaaa ccatcaaagg gattaaacgc
     3061 tataaacaga gccagctact ggcggtgggg aatccaaccg atctggcgat tgaagccatc
     3121 cgtagtctgc gcaccagtct gcacttcgcg atgatgcagg cacagaacaa tgtgttgatg
     3181 atgaccgggg ttagcccgtc aatcggtaaa acctttgtct gcgccaacct ggcggcggtg
     3241 atcagtcaga ccaataaacg ggtgttgttg atcgactgcg atatgcgcaa aggctacacc
     3301 cacgagctgt tgggcactaa taacgtcaat ggcctgtcgg aaattctgat tggtcagggc
     3361 gagattacta ccgctgctaa accgacctct attgccaaat ttgacctgat cccgcgcggt
     3421 caggtaccgc caaatccttc ggaactgttg atgagcgaac gttttgccga actggtgaac
     3481 tgggcgagta aaaactacga cctggtgttg attgatacgc cgccgattct ggcagtgacc
     3541 gatgcggcaa ttgttggtcg tcatgtcgga accacgttga tggtggcgcg ttatgcggtc
     3601 aacacattga aagaagtgga aaccagtctg agccgctttg agcaaaacgg tattccggtg
     3661 aaaggggtga ttctgaactc catcttccgt cgcgccagcg cgtatcagga ttatggctat
     3721 tacgaatacg aatataagtc ggacgcgaaa taacgaaatc cgctgtcgga tgcgatgctg
     3781 acgcatctta tccgacctac gttcacgccg catcaggcaa gcaaacccgc tccgtaggtc
     3841 ggataaggcg ttcacgccgc atccggcaac agaacctgta aaccagctta taaaccggca
     3901 gaacatctcg acactccggg agaacaacct atgaaagaca atccgctgat ttcaatctat
     3961 atgccgacct ggaaccgtca acaactggcg attcgggcga taaaatcggt cctgcgccag
     4021 gactacagta actgggagat gatcatcgtg gatgattgct ccacctcctg ggaacaacta
     4081 caacagtacg tcaccgccct caacgatccg cgtatcactt acattcacaa cgacattaac
     4141 agcggggcgt gcgcggtacg taaccaggcg attatgctgg cgcaagggga atatatcacc
     4201 gggatcgatg atgatgatga atggacaccc aaccgtctga gcgtcttcct cgcccataaa
     4261 cagcaactgg tcacacacgc ctttttgtac gctaacgact acgtttgcca gggcgaagtc
     4321 tattcccaac cggcaagttt gccactgtat cccaaatcgc cgtattcacg ccgcctgttc
     4381 tacaaacgca atatcattgg caatcaggtc tttacctggg cgtggcgttt caaagagtgt
     4441 ttgttcgata ccgaactgaa agcggcacag gattacgaca ttttcctgcg gatggtggtg
     4501 gagtatggcg aaccgtggaa agtggaagag gcgacgcaga tcctgcatat caatcacggc
     4561 gaaatgcaga tcacctcgtc accgaaaaaa ttctccggat acttccactt ttaccgtaag
     4621 cacaaagaca aatttgaccg cgccagcaaa aaatatcagc tgtttaccct ctaccagatc
     4681 cgcaataaac gcatgacctg gcgcaccttg ttgacgctgc tgtcggtgcg taacggcaaa
     4741 cgtctggctg atggtattcg ggggcgctaa agatgctgga agatctgcgc gccaacagct
     4801 ggagtttacg cccgtgctgc atggttcttg cctatcgcgc cgctcatttt tgttcggtgt
     4861 ggcgcaaaaa gaacgtcctc aacaatcttt gggcggcccc gctgctggtg ctgtatcgca
     4921 ttatcaccga atgctttttc ggttatgaaa tccaggccgc cgcgaccatt ggccgccgtt
     4981 tcactatcca tcacggttac gccgtagtga tcaataaaaa cgtggtagcg ggggatgatt
     5041 tcaccattcg ccacggcgtc actatcggca atcgtggtgc cgataacatg gcatgtccac
     5101 acattggcaa cggcgtcgaa ctcggtgcca acgtcattat tcttggtgat atcacgcttg
     5161 gtaacaacgt caccgtgggc gcgggcagcg tggtgctcga ctctgtcccg gacaacgcgc
     5221 tggtggtggg agaaaaagcg cgagtgaagg taattaaatg aatattctgc aattcaatgt
     5281 gcgactggcg gaaggcgggg cggcaggtgt ggcgttagat ctccaccagc gcgcgctgca
     5341 acaggggctg gcgtcacatt ttgtctacgg ctacggcaaa ggcggcaaag agagcgtcag
     5401 ccatcaaaac tatccgcagg tcatcaaaca tacgccgcgg atgaccgcga tggcgaacat
     5461 tgcgttgttt cgtctgctta atcgcgatct gttcggcaat ttcaatgagt tatatcgcac
     5521 cattactcgc acaccgggtc cggtggtcct gcattttcat gtgctgcaca gctactggtt
     5581 gaatcttaag agcgtggtgc gcttttgcga aaaagtgaaa aaccataaac cggatgtcac
     5641 tctggtctgg acgttacacg atcactggag cgttaccgga cgctgcgcct ttaccgacgg
     5701 ttgcgaaggc tggaaaacgg gctgccagaa atgcccgacc ttaaataatt atccgccggt
     5761 gaagattgat cgcgcacacc aacgggtggc gggcaaacgg cagttattca gcgagatgct
     5821 ggcgctgggc tgtcagttta tttcccccag ccagcatgtg gcagacgctt tcaatagtct
     5881 gtacggtcca gggcgttgcc ggattatcaa taacggtatt gatatggcaa ccgaagcgat
     5941 tctggcggac ttgcctccgg tgcgcgaaac ccagggcaag ccgaaaatcg cggtggtggc
     6001 gcatgacctg cgttacgacg gcaaaactaa ccagcaactg gtacgcgaga tgatggcgct
     6061 gggcgacaaa atcgaactgc atactttcgg taagttctcg ccgttcaccg ctggtaacgt
     6121 tgttaatcac ggctttgaaa ctgacaaacg taaactgatg agcgcgctca accagatgga
     6181 tgcgctggtg ttcagttctc gcgtcgataa ctacccgctg attttgtgtg aggcgttatc
     6241 gattggcgtg ccggtgattg ccacccatag cgacgcggcg cgggaagtgc tgcaaaaatc
     6301 cggcggtaaa accgtcagcg aagaagatgt gctgcaactg gtgcagtcaa gcaaaccgga
     6361 aatcgcacag gcgatatttg gtaccacgct ggctgagttc agccagcgca gccgcgccgc
     6421 ctacagtgga caacagatgc tggaggagta tgtcaacttc tatcagaatc tgtagctacc
     6481 tgctgctgcc gctgatctac ctgctggtta acgtcaaaat cgcccagctt ggcgaaagtt
     6541 ttcccatcac catcgtcact tttttacctg tcttgttact gctgttttta gaacgcatca
     6601 gcgttaaaaa attgatgatt gccttaggga ttggtgcggg actcacggcg tttaactacc
     6661 tgtttggtca gtcgttggat gccggtaaat acgtcacttc aactatgctg tttgtctata
     6721 ttgtgatcat tattggcatg gtgtggagta ttcgctttaa aacgatttcg ccccacaacc
     6781 accgcaagat attacgtttc ttttatctgg tggtcgggct ggtggtggtg ctggcggcgg
     6841 tggagatggc gcaaattatc ctcaccggcg gtagcagtat tatggagtcg atttcgaaat
     6901 atctgattta cagcaacagc tatgtgctga atttcattaa attcggcggc aagcgcacga
     6961 cagcacttta tttcgaaccg gcatttttcg ctctggcatt aatctcaatt tggctcagca
     7021 tcaaacagtt tggtatcaaa acgcctaaaa ccgatgctat gattcttgca gggataatat
     7081 tatccggatc gttttcaggg gttatgacct ttatcctgtt ttatttgctg gagtgggcat
     7141 ttcaatatct gaataaagag gcgattaaga aaaagttacc gttagcattg atttctctgg
     7201 ctgtattcct ggttggtgtg gtaatcgcgt ttccttatat ttccacccgt ctgggcgatt
     7261 taggtacgga aggatcgtca tcgtattatc gtattgtcgg tccgctggtg atggtcggtt
     7321 attctttgac ccatattgac ggtgtagtca gatttggctc actttatgaa tatgtcgcat
     7381 cattcggaat atttaacggt gcggatgtcg gaaaaaccat agacaatggt ttgtatctgc
     7441 tgattattta tttttcctgg ttcgcagtgt ttttatcgct gtggtacatg gggaaagtga
     7501 taaaaatgat gatcaacgct tttggtgata accgcaattt tcgcgtgcaa ttatatcttt
     7561 ttactccggt atcgctgttt tttaccggtt cgatatttag cccggaatat gcatttttaa
     7621 tcgtctgtcc gtttattttg cgaaaagcgt taaatattac gaggtaagaa taagaacatg
     7681 ttgcttagca taatcactgt cgcgtttcgt aacctcgcag ggatagtcaa aacacatgcc
     7741 tcgttggcgc atctggcgca ggctgacgat atcagcttcg aatggattgt tgtcgatggc
     7801 ggttccaatg acggcacccg tgagtatctg gaaaatctca atggtatcta taacctacgc
     7861 tttgtcagcg agccagataa cggtatctac gacgccatga acaaaggcat tgcgatggcg
     7921 catggcaagt tcgcgttgtt tctcaactca ggcgatattt ttcatcagga tgccgcaaat
     7981 tttgtccgta agttaaaagt gcaaaaagat aacatgatga tcaccggcga tgcgctgctg
     8041 gattttggcg acgggcataa aattaaacgt agcgccaaac cgggttggta tatttatcac
     8101 agcctgcccg ccagtcatca ggcgatattt ttcccggtgt ccggcttgaa aaaatggcgt
     8161 tatgacctgg aatataaagt ttcttccgac tacgcgctgg cagccaaaat gtataaagcc
     8221 ggttatgcat ttaaaaaact caatggcctg gtgtctgaat tttctatggg tggggtatca
     8281 accaccaata atatggaatt gtgtgctgac gccaaaaaag tccaacggca aatattacat
     8341 gtgcctggct tctgggcaga attatcctgg catttacgcc aacgtactac ctcaaagacg
     8401 aaagccttat ataacaaaag ctgaatataa gggaaaacga tgcaagattt aagcggtttc
     8461 tcggtaccga aagggttccg gggtggcaac gctattaaag tgcaattgtg gtgggcagta
     8521 caggcaacaa tatttgcctg gtcgccacaa gtattgtatc gctggcgggc atttttatta
     8581 cgtttattcg gcgcaaaaat aggaaaaaac gtagttattc gtccgtcagt aaaaattacc
     8641 tatccgtgga aattaacctt aggtgattac gcgtgggtcg gcgatgacgt caatttatat
     8701 accctcggtg aaataaccat tggcgcacat tcggtgatat cgcaaaaaag ttatttatgc
     8761 accggtagcc acgaccatgc aagtcaacat ttcaccatta acgccacgcc tattgtgatt
     8821 ggcgagaaat gctggctggc aaccgatgtc tttgttgccc caggcgtcac gatcggcaac
     8881 ggcaccgtcg tgggtgcacg aagcagtgtt tttaaatcgc ttccggcaaa tgtggtttgc
     8941 cgggggaatc ccgcagtggt gatacgcgaa cgcgttgaaa ctgaataaat tcaaaaaata
     9001 cagaggaata agacatgtca aaagtcgctc tcatcaccgg tgtaaccgga caagacggtt
     9061 cttacctggc agagtttctg ctggaaaaag gttacgaggt gcatggtatt aagcgtcgtg
     9121 catcgtcatt caacaccgag cgcgtggatc acatttatca ggatccgcac acctgcaacc
     9181 cgaaattcca tctgcattat ggcgacctga gtgatacctc caacctgacg cgtattttgc
     9241 gtgaagtgca gccggatgaa gtgtacaacc tgggcgcaat gagccacgtt gcggtttctt
     9301 ttgagtcacc ggaatatacc gcagacgtcg atgcgatggg tacgctgcgc ctgctggagg
     9361 cgatccgctt cctcggtctg gaaaagaaaa cccgtttcta tcaggcttct acttctgaac
     9421 tgtacggtct ggtgcaggaa attccgcaga aagagaccac gccgttctac ccacgctctc
     9481 cgtatgcggt cgccaaactg tacgcctact ggatcaccgt taactaccgc gaatcctacg
     9541 acatgtacgc ctgtaacggt attctcttca accatgaatc cccgcgccgt ggcgagactt
     9601 tcgttacccg caaaatcacc cgcgcaatcg ccaacatcgc ccaggggctg gagtcgtgcc
     9661 tgtatctcgg caatatggat tccctgcgtg actggggcca cgccaaagac tacgtaaaaa
     9721 tgcagtggat gatgctgcaa caggaacaac cggaagattt cgttatcgcg accggcgttc
     9781 agtactccgt acgtcagttc gtggaaatgg cggcagcaca gttgggcatc aaactgcgct
     9841 ttgaaggcac gggtgttgaa gagaagggca ttgtggtttc cgtcaccggg catgacgcgc
     9901 cgggcgttaa accgggcgat gtgattatcg ccgttgaccc gcgttacttc cgtccggcgg
     9961 aagttgaaac gctgctcggc gacccgacca aagcgcacga aaaactgggc tggaaaccgg
    10021 aaatcaccct cagagagatg gtgtctgaaa tggtggctaa tgacctcgaa gcggcgaaaa
    10081 aacactctct gctgaaatct cacggctacg acgtggcgat cgcgctggag tcataagcat
    10141 gagtaaacaa cgcattttta ttgctggcca tcgcgggatg gtcggttccg ccatcaggcg
    10201 gcagctcgaa cagcgcggtg atgtagagct ggtattacgc acccgcgacg agctgaacct
    10261 gctggacagc cgcgcggtgc atgatttctt tgccagcgaa agcattgacc aggtctatct
    10321 ggcggcggcg aaagtgggcg gcattgttgc caacaacacg tatccggcgg atttcatcta
    10381 ccagaacatg atgattgaga gcaacatcat tcacgccgcg catcagaacg acgtgaacaa
    10441 actgctgttt ctcggatcgt cctgcatcta cccgaaactg gcaaaacagc cgatggcaga
    10501 aagcgagttg ttgcagggca cgctggagcc gactaacgag ccttatgcta ttgccaaaat
    10561 cgccgggatc aaactgtgcg aatcttacaa tcgccagtac ggacgcgatt accgctcagt
    10621 catgccgacc aacctgtacg ggccgcatga caacttccac ccgagtaact cccatgtgat
    10681 acctgcattg ctgcgtcgct tccacgaggc gacggcacag aatgcgccgg acgtggtggt
    10741 atggggcagc ggtacaccga tgcgtgaatt cctgcacgtc gatgatatgg cggcggcgag
    10801 cattcatgtc atggagctgg cgcacgaagt ctggctggaa aacacccagc cgatgctgtc
    10861 gcacattaac gtcggcacgg gcgttgactg cactatccgc gagctggcgc aaaccatcgc
    10921 caaagtggtg ggttacaaag gtcgggtggt ttttgatgcc agtaaaccgg atggtacacc
    10981 gcgcaaactg ctggatgtga cgcgcctgca tcagcttggc tggtatcacg aaatctcact
    11041 ggaagcgggg cttgccagca cttaccagtg gttccttgag aatcaagacc gctttcgggg
    11101 gtaatgatgt ttttacgtca ggaagacttt gccacggtag tgcgctccac accgcttgtc
    11161 tctctcgact ttattgtcga gaacagtcgc ggcgagtttc tgcttggcaa aagaaccaac
    11221 cgcccggcgc agggttactg gtttgtgccg ggagggcgcg tgcagaaaga cgaaacgctg
    11281 gaagccgcat ttgagcggct gacgatggcg gaactggggc tgcgtctgcc gataacagca
    11341 ggccagtttt acggtgtctg gcagcacttt tatgacgata acttctccgg cacggatttc
    11401 accactcact atgtggtgct cggttttcgc ttcagagtag tggaagaaga gctgttactg
    11461 ccggatgagc agcatgacga ttaccgctgg ctgacgccgg acgcactgct cgccagtgac
    11521 aatgttcacg ctaacagccg cgcctatttt ctcgctgaga agcgtgccgg agtacccgga
    11581 ttatgaaaat actggtctac ggcattaact actcgccgga gttaaccggc atcggcaaat
    11641 acaccggcga gatggtggaa tggcttgcgg cgcaaggtca tgaggtgcgg gttattaccg
    11701 caccgcctta ctacccgcaa tggcaggtgg gcgagaacta ttccgcctgg cggtacaaac
    11761 gagaagaggg ggccgccacg gtgtggcgct gcccgctgta cgtgccaaaa cagccgagca
    11821 ccctgaaacg cctgttgcat ctcggcagtt ttgccgtcag cagttttttc ccgttgatgg
    11881 cgcaacgtcg ctggcagccg gatcgcatta ttggcgtggt gccaacgctg ttttgcacgc
    11941 cgggaatgcg cctgctggcg aaactctctg gggcgcgcac ggtgctccat attcaggatt
    12001 acgaagtgga cgccatgctg gggctgggcc ttgccggaaa aggtaaaggc ggcaaagtgg
    12061 cacagctggc aacggcgttc gaacgtagcg gactgcataa cgtcgataac gtctccacga
    12121 tttcgcgttc gatgatgaat aaagctatcg aaaaaggcgt ggcggcggaa aacgtcatct
    12181 tcttccccaa ctggtcggaa atcgcccgtt ttcagcatgt tgcaaatgcc gatgttgatg
    12241 cccttcgtaa ccagcttggc ctgccggata acaaaaaaat cattctttac tccggcaata
    12301 ttggtgaaaa gcaggggctg gaaaacgtta ttgaagctgc cgatcgcctg cgcgatgaac
    12361 cgctgatttt tgccattgtc gggcagggcg gcggcaaagc gcggctggaa aaaatggcgc
    12421 agcagcgtgg actgcgcaac atgcaatttt ttccgctgca atcgtatgac gctttacccg
    12481 cactgctgaa gatgggcgat tgccatctgg tggtgcaaaa acgcggcgcg gcagatgccg
    12541 tattgccgtc gaaactgacc aatattctgg cggtaggcgg taacgcggtg attaccgcag
    12601 aagcccacac cgaactggga cagctttgcg aaacctttcc gggcattgcg gtttgcgtgg
    12661 aaccggaatc ggtcgaggcg ctggtggcgg ggattcgtca ggcgctcctg ctgcccaaac
    12721 acaacacggt ggcacgtgaa tatgccgaac gcacgctcga taaagagaac gtgttacgtc
    12781 aatttataaa tgatattcgg ggataattat ggcgcagtcg aaactctatc cagttgtgat
    12841 ggcaggtggc tccggtagcc gcttatggcc gctttcccgc gtactttacc ccaagcagtt
    12901 tttatgcctg aaaggcgatc tcaccatgct gcaaaccacc atctgccgcc tgaacggcgt
    12961 ggagtgcgaa agcccggtgg tgatttgcaa tgagcagcac cgctttattg tcgcggaaca
    13021 gctgcgtcaa ctgaacaaac tcaccgagaa tattattctc gaaccggcag ggcgtaacac
    13081 tgcacctgcc attgcgctgg cggcgctggc ggcaaaacgt catagcccgg agagcgaccc
    13141 attaatgctg gtgctggcgg cggatcatgt gattgccgat gaagacgctt ttcgcaccgc
    13201 cgtgcgtaat gccatgccgt atgccgaagc gggcaagctg gtgaccttcg gcattgtgcc
    13261 ggatctacct gaaaccggat atggctatat tcgtcgcggt gaagtgtctg cgggtgagca
    13321 ggatacggtg gcctttgaag tggcgcagtt tgtcgaaaaa ccgaatctgg aaaccgcgca
    13381 ggcctatgtg gcaagcggcg aatattactg gaacagcggt atgtttctgt tccgcgccgg
    13441 acgctatctc gaagaactga aaaaatatcg cccggatatt ctcgacgcct gtgaaaaagc
    13501 gatgagcgcc atcgatccgg atctcgattt tattcgtgtg gatgaagaag cgtttctcgc
    13561 ctgcccggaa gagtcggtgg attacgcagt catggaacgt acggcagatg ctgttgtggt
    13621 gccgatggat gcgggctgga gcgatgtcgg ttcctggtcc tctttatggg agatcagcgc
    13681 ccacaccgcc gagggcaacg tttgccacgg cgatgtgatt aatcacaaaa ctgaaaacag
    13741 ctatgtgtat gccgaatctg gcctggtcac caccgtcggg gtgaaagatc tggtggtggt
    13801 gcagaccaaa gatgcggtgc tgattgccga ccgtaacgcg gtgcaggatg tgaaaaaagt
    13861 ggtcgagcag atcaaagccg atggtcgcca tgagcatcgg gtgcatcgcg aagtgtatcg
    13921 tccgtggggc aaatatgact ctatcgacgc gggcgaccgc taccaggtga aacgcatcac
    13981 cgtgaaaccg ggcgaaggct tgtcagtaca gatgcaccat caccgcgcgg aacactgggt
    14041 agtggtcgcg ggaacggcaa aagtcactat cgacggtgat atcaaactcc ttggtgaaaa
    14101 cgagtccatt tatattccgc tgggggcgac gcattgcctg gaaaacccgg ggaaaattcc
    14161 gctcgattta attgaagtgc gctccggctc ttatcttgaa gaggatgatg tggtgcgctt
    14221 cgcggatcgc tacggacggg tgtaaacgtc gcatcaggcg ctgtttgtcg gatgcggcgt
    14281 gaacgcctta tccgacctac ggttcgggtt tgtaggcctg ataagacgcg gcagcgtcgc
    14341 atcaggcaat gaatgcgaaa ccgcgatgta aataacgaca aaaacaaaat tggccgtttc
    14401 ggtcagggcc aactattgcc tgaaaaaggg taacgatatg aaaaaattaa cctgctttaa
    14461 agcctatgat attcgcggga aattaggcga agaactgaat gaagatatcg cctggcgcat
    14521 tggtcgcgcc tatggcgaat ttctcaaacc gaaaaccatt gtgttaggtg gtgatgcccg
    14581 cctcaccagc gaaaccttaa aactggcgct ggcgaaaggt ttacaggatg cgggcgtcga
    14641 tgtgctggat attggtatgt ccggcaccga agaaatctat ttcgccacgt tccatctcgg
    14701 cgtggatggc ggcattgaag ttaccgccag ccataacccg atggattaca acggtatgaa
    14761 actcgtgcgc gagggggcac gcccgatcag cggggatacc ggactgcgcg acgtccagcg
    14821 tctggcagaa gccaacgact ttcctcccgt tgatgaaacc aaacgcggtc gctatcagca
    14881 aatcaatctg cgtgacgctt acgttgatca cctgttcggt tatatcaacg tcaaaaacct
    14941 cacgccgctc aagctggtga tcaactccgg gaacggcgca gcgggtccgg tggtggacgc
    15001 cattgaagcc cgctttaaag ccctcggcgc accagtggaa ttgatcaaag tgcacaacac
    15061 gccggacggc aatttcccca acggaattcc taacccgctg ctgccggaat gccgcgacga
    15121 cacccgcaat gcggtcatca aacacggcgc ggatatgggc attgcctttg atggcgattt
    15181 tgaccgctgt tttctgtttg acgaaaaagg gcagtttatc gagggctact acattgtcgg
    15241 cctgctggca gaagcgttcc tcgaaaataa tcccggcgcg aagatcatcc acgatccgcg
    15301 tctctcctgg aacaccgttg atgtggtgac tgccgcaggc ggcaccccgg taatgtcgaa
    15361 aaccggacac gcctttatta aagaacggat gcgcaaggaa gacgccatct acggtggcga
    15421 aatgagcgcc caccattact tccgtgattt cgcttactgc gacagcggca tgatcccgtg
    15481 gctgctggtc gccgaactgg tgtgcctgaa agggaaaacg ctgggcgaac tggtgcgtga
    15541 ccggatggca gcatttccgg caagcggtga gatcaacagc aaactggcgg accctgttga
    15601 ggcgattaac cgcgtcgaac agcattttag ccgcgaggcg ctggcggtgg atcgcaccga
    15661 tggcatcagc atgacctttg ccgactggcg ctttaacctg cgcacctcca acaccgaacc
    15721 ggtggtgcgc ctgaatgttg aatcgcgcgg tgatgtgccg ctgatggaag cgcgaacgcg
    15781 aactctgctg acgttgctga acgagtaatg tcggatcttc ccttacccca ctgcgggtaa
    15841 ggggctaata acaggaacaa cgatgacaaa tctaaaaaag cgcgagcgag cgaaaaccaa
    15901 tgcatcgtta atctctatgg tgcaacgctt ttcagatatc accatcatgt ttgccggact
    15961 atggctggtt tgcgaagtca gcggactgtc attcctctac atgcacctgt tggtggcgct
    16021 gattacgctg gtggtgttcc agatgctggg cggcatcacc gatttttatc gctcatggcg
    16081 cggtgttcgg gcagcgacag aatttgccct gctgctacaa aactggacct taagcgtgat
    16141 tttcagcgcc gggctggtgg cgttcaacaa tgatttcgac acgcaactga aaatctggct
    16201 ggcgtggtat gggctgacca gtatcggact ggtggtttgc cgttcgtgta ttcgcattgg
    16261 ggcgggctgg ctgcgtaatc atggctataa caagcgcatg gtcgcggtgg cgggggattt
    16321 agccgccgga caaatgctga tggagagctt ccgtaatcag ccgtggttag ggtttgaagt
    16381 ggtgggcgtt taccacgacc cgaaaccggg tggcgtttct aacgactggg cgggcaatct
    16441 gcaacagctg gtcgaggatg cgaaagcggg caagattcat aacgtctata tcgcgatgca
    16501 aatgtgcgac ggcgcacgcg tgaaaaaact ggtccatcaa ttggcggaca ccacctgttc
    16561 ggtgctgctg atccccgatg tctttacctt caacattctc cattcccgta tcgaagagat
    16621 gaacggcgta ccggtggtgc cgctgtatga cacgccgctt tccggggtta accgcctgct
    16681 caaacgtgcg gaagatattg tgctggcgac gcttatcctg ctgctgatct ccccggtgct
    16741 gtgctgcatt gcgctggcgg tgaaactgag ctcaccgggg ccggttattt tccgccagac
    16801 tcgctacggc atggacggca agccgatcaa agtgtggaag ttccgttcca tgaaagtgat
    16861 ggagaacgac aaagtggtga cccaggcgac gcagaacgat ccgcgcgtca ccaaagtggg
    16921 gaactttctg cgccgcacct cgctggatga attgccgcag tttatcaatg tgctgactgg
    16981 cgggatgtcg attgtcgggc cacgtccgca cgcggtggcg cataacgaac agtatcgcca
    17041 gctcattgaa ggctacatgc tgcgccataa agtgaaaccg ggcattaccg gctgggcgca
    17101 gattaacggc tggcgcggcg aaaccgacac actggagaaa atggaaaaac gcgtcgagtt
    17161 cgaccttgag tacatccgcg aatggagcgt ctggttcgat atcaaaatcg ttttcctgac
    17221 ggtattcaag ggcttcgtta acaaagcggc atattgatat gaacttacgt gaaaaaacca
    17281 tcagcggcgc gaagtggtcg gcgattgcca cggtgatcat catcggcctc gggctggtgc
    17341 agatgaccgt gctggcgcgg attatcgaca accaccagtt cggtttgctt accgtgtcgc
    17401 tggtgatcat cgcgctggca gatacgcttt ctgacttcgg catcgctaac tcgattattc
    17461 agcgaaaaga aatcagccac cttgaactca ccacgctgta ctggctgaac gtcgggctgg
    17521 ggatcgtggt gtgcgtggcg gtgtttttgt tgagtgatac cattggcgac gtgctgaata
    17581 acccggacct ggcaccgttg attaaaacat tgtcgctggc gtttgtggtg atcccccacg
    17641 ggcagcagtt ccgcgcgttg atgcagaagg agctggagtt caacaaaatc ggcatgatcg
    17701 aaaccagtgc ggtgctggcg ggcttcactt ttacggtggt tagcgcccat ttctggccgc
    17761 tggcgatgac cgcgatcctc ggttatctgg tcaatagtgc ggtaagaacg ctgctgtttg
    17821 gctactttgg ccgcaaaatt tatcgccccg gtctgcattt ctcgctggcg tcggtggcac
    17881 caaacttacg ttttggtgcc tggctgacgg cggacagcat catcaactat ctcaatacca
    17941 acctttcaac actcgtgctg gcgcgtattc tcggcgcggg cgtggcaggg ggatacaacc
    18001 tggcgtacaa cgtggccgtt gtgccgccga tgaaactgaa ccccatcatc acgcgcgtgt
    18061 tgtttccggc atttgccaaa attcaggacg acaccgaaaa gctgcgcgtc aacttctaca
    18121 agctgctgtc ggtggtgggg attatcaact ttccggcgct gctggggctg atagtggtgt
    18181 cgaataactt tgtaccgctg gtatttggtg agaagtggaa cagcattatt ccggtgctgc
    18241 aattgctgtg tgtggtgggg ctgctgcgct cagtggggaa cccgattggt tcgctgctga
    18301 tggcgaaagc gcgggtcgat atcagcttta aattcaacgt attcaaaacc tttctgttta
    18361 ttccggcgat tgttattggt gggcagatgg cgggcgcaat cggcgtcacg cttggcttcc
    18421 tgctggtgca gattatcaac accattctga gctatttcgt gatgattaaa ccggtgctcg
    18481 gttccagtta tcgccagtac atcctgagtt tgtggctgcc gttttatctc tcgctgccga
    18541 cgctggtggt cagttatgcg ctgggcattg tgctgaaagg gcaactggcg ctggggatgc
    18601 tgctggcggt gcaaatagcc acgggcgtgc tggcgtttgt ggtgatgatt gtgctgtcgc
    18661 gccatccgct ggtggtggaa gtgaagcgtc agttttgtcg cagcgaaaaa atgaaaatgc
    18721 ttttacgggc ggggtgaatg gttgttttcg gttccggcaa cggttgccgg atgcggcgta
    18781 aaacgcctta tccgtcctgg ggggctgggc ttggatcttg taggcctgat aagacgcgcc
    18841 agcgtcgcat caggcactga atgccggatg cggcgtgaac gccttatccg gcctacggtt
    18901 ctgtgctcgg atcttgtagg cctgataaga cgcgtcagcg tcgcatcagg cactgaatgc
    18961 cggatgcggc gtaaaacgcc ttatccgtcc tacggctctg tgctcgggtc ttgtaggcct
    19021 gataagacgc gtcagcgtcg catcaggcac tgaatgccgg aagcggcgtg aacgccttat
    19081 ccgtcctacg gttctgggct tggatcttgt aggcctgata agaggcgtca gcgtcgcatc
    19141 aggtactgaa tgccggatgc ggcgtgaacg ccttatccgt cctacggttc tgggcttgga
    19201 tcttgtaggc ctgataagac gcgtcagcgt cgcatcaggc actgaatgcc ggatgcggcg
    19261 tgaacgcctt atccgtccta cggttctgtg ctcggatctt gtaggcctga taagacgcgt
    19321 tagcgtcgca tcaggcaaaa aacggcaacg aacacaacaa acacagcctc acctggcatc
    19381 aaatttatga ggaaaaaatg aaattactta ttctgggcaa ccacacttgc ggcaatcgtg
    19441 gcgatagcgc catcctgcgt ggcttacttg atgccatcaa tatcctcaat ccacatgccg
    19501 aagtggacgt gatgagccgc tatccggtca gttcttcctg gctgctcaac cgcccggtaa
    19561 tgggcgatcc gctgttcctg caaatgaaac aacacaacag cgcggcgggc gttgtcgggc
    19621 gtgttaaaaa agtcctccgt cgccgctacc agcaccaggt attgctctca cgcgtcaccg
    19681 acactggcaa gctgcgtaat atcgccatcg cccagggatt cactgacttc gtgcgactgc
    19741 tgtcaggtta cgacgccatt attcaggtcg gcggatcgtt ttttgtcgat ctctacggcg
    19801 tgccgcagtt tgaacatgcg ctttgcactt tcatggcgaa aaagccgctg tttatgattg
    19861 gtcacagcgt cggccccttc caggatgagc aatttaacca actggcgaac tatgtctttg
    19921 gtcactgcga cgcgctgatc ctgcgcgaat cggtcagcct tgatttgatg aaacgcagca
    19981 atatcaccac tgcaaaagtg gaacatggcg tcgataccgc gtggctggtc gatcaccaca
    20041 ttgaagactt caccgccagc tatgctgttc aacactggct ggatgttgcc gcacaacaga
    20101 aaacggtggc aattaccctg cgcgaactgg caccgttcga caaacgtctc ggcaccactc
    20161 aacaagcgta tgaaaaagcc tttgccgggg tggtcaatcg cattctcgat gaagggtatc
    20221 aggtgattgc gctctccacc tgtacgggca tcgacagtta taacaaagac gaccgcatgg
    20281 tggcgctcaa tctgcgccag cacatcagcg atcctgcccg ttatcacgta gtgatggatg
    20341 aactcaacga tctggaaatg ggcaaaattc tgggggcctg tgaacttacc gtcggtacgc
    20401 gcctgcactc tgctattatc tcgatgaact ttgccactcc ggcgattgcc atcaactacg
    20461 aacataaatc cgccgggatc atgcaacaac tggggctacc agaaatggcg attgatatcc
    20521 gtcatttatt agacggcagc ctgcaagcga tggttgcgga taccttaggc cagcttccgg
    20581 cactgaacac gcgacttaac gaagccgtca gtcgcgaacg tcagacggga atgcagatgg
    20641 tgcaatctgt gcttgaacgc atcggggagg tgaaatgaag gtcggcttct ttttactgaa
    20701 atttccgttg tcgtcggaaa ccttcgttct taaccaaatc actgcgttta ttgatatggg
    20761 atttgaggtg gagattatcg cgctgcaaaa aggcgacaca caaaacaccc acgcggcatg
    20821 gacgaaatac aaccttgctg ccagaacccg ctggttacag gacgaaccta cgggcaaagt
    20881 ggcgaaactg cgccaccgcg ccagccagac cttatgcggc attcatcgta aaaatacctg
    20941 gcaggcgctc aacctcaaac gctatggcgc cgagtcgcgg aacctgattt tgtctgctat
    21001 ttgcggtcag gtcgcaacac cgtttcatgc cgatgtcttt atcgctcact ttggtccggc
    21061 aggggtaacc gcagcaaaac tccgcgaact gggtgtcatt cgcggcaaaa ttgccactat
    21121 cttccacggt attgatatct ccagtcggga ggtgctcaac cattacactc ccgaatatca
    21181 gcaactgttc cgccgtggcg acctgatgtt accgataagc gatctgtggg ccggaaggct
    21241 gcaaaaaatg ggctgcccga gggaaaaaat cgccgtatcg cgtatgggcg tggacatgac
    21301 gcgctttagc ccgcgtccgg tgaaagcgcc tgcaacgccg ctggaaatca tttccgtcgc
    21361 acgcttaacc gagaaaaaag gcctgcatgt ggcgatcgaa gcctgccgtc agttgaaaga
    21421 gcagggcgtg gcatttcgct atcgcatcct cggcattggc ccgtgggaac gacgcctgcg
    21481 caccctcatc gaacaatatc aactggaaga tgtgatagag atgccgggct ttaaaccgag
    21541 ccatgaagtg aaggcgatgc tcgacgacgc ggatgtcttc ctgttgccat cggttacagg
    21601 tgcggacggc gatatggaag gtattccggt ggcactaatg gaagcgatgg cggtcggcat
    21661 tccggtggtt tctactctgc atagcggaat accggaactg gtggaggccg ataaatccgg
    21721 ctggctggtg cctgagaacg atgctcgcgc actggcgcaa cgactggcgg cgtttagcca
    21781 actggacacc gacgaactgg ctccggttat caaacgtgct cgcgaaaaag tcgaacacga
    21841 ttttaaccag caggtgatca atcgagaact cgccagcttg ctgcaggctt tatagaggag
    21901 aacgtatgcc atttaaaaaa ctctcccgac gcaccttcct gacggcaagc tcggcgcttg
    21961 ccttcctcca tacccccttc gcccgcgcac ttccagcccg acaaagcgtt aatatcaacg
    22021 attacaatcc gcacgactgg atcgcctcat ttaaacaagc cttcagtgaa gggcaaacgg
    22081 tcgtcgtgcc tgccggattg gtttgtgaca atatcaacac cggcatcttc attcctcctg
    22141 gaaaaacctt acacatcctt ggaagcctgc gcggcaacgg cagagggcga tttgtcttac
    22201 aggacggcag ccaggtgaca ggggaggagg gcggcagtat gcataacatc accctggatg
    22261 tgcgcggttc tgactgcatc atcaaagggt tggcgatgag cggttttggc ccggtaacgc
    22321 agatttatat cggcggcaaa aataaacgcg tcatgcgcaa cttgaccatc gataacctga
    22381 ccgtcagcca cgctaattac gccattttac gccagggatt tcataaccag attatcggtg
    22441 ccaacatcac caactgtaag ttcagcgact tacagggcga cgccattgaa tggaatgtgg
    22501 cgattaacga ccgcgatatt ttgatatccg accacgttat tgagcgcatc aactgtacca
    22561 acggcaaaat caactggggc atcggcatag gccttgcggg aagcacttac gataacaact
    22621 atccggaaga ccaggcagtg aaaaactttg tcgtggcgaa tatcacggga tcagattgtc
    22681 gacagttgat acatgttgaa aatggtaaac attttgttat tcgtaatatc aaagcccgca
    22741 atatcacgcc ggatttcagt aagaaagcag gtattgataa cgccacggtc gctatttacg
    22801 gttgtgacaa tttcgtgatt gataatattg aaatgattaa tagcgccggg atgttaattg
    22861 gctatggggt aattaaaggc aaatatctct cgataccgca aaatttccga gtgaataata
    22921 ttcaactgga taacactcac cttgcttata aattgcgcgg catccaaatc tccgccggga
    22981 atgctgtctc ctttgtggcg ctgactaaca ttgagatgaa gcgtgcctcg ctggagcttc
    23041 acaataaacc gcaacatctt tttatgcgaa atatcaaagt gatgcaggaa tcctctgttg
    23101 gacccgcatt gagcatgaac ttcgacatgc gcaaagacgt tcgcggcgtc tttatggcga
    23161 aaaaagaaac actgctgtct cttgcaaatg ttcatgcgat gaatgaaaga gggcaaagct
    23221 ccgtcgatat cgacagggtt aatcaccata ttgttaatgt ggaaaagatt aactttagat
    23281 tgccagaacg gagggagtag atttgcgacc attcataaac ttactggata taatgttcta
    23341 aaagattata atcctcacac taactgttaa agctggggct gggcagcatt gactactcag
    23401 ggaaggtact catctttatc ttgtgtttct tacgtaacaa ccagactaaa gtgatttatg
    23461 aacgataacg ttttgctcat aggagcttcc ggattcgtag gaacccgact acttgaaacg
    23521 gcaattgctg actttaatat caagaacctg gacaaacagc agagccactt ttatccagaa
    23581 atcacacaga ttggcgatgt tcgtgatcaa caggcactcg accaggcgtt agctggtttt
    23641 gacactgttg tgctactggc agcggaacac cgcgatgacg tcagccctac ttctctctat
    23701 tatgatgtca acgttcaggg tacccgcaat gtgctgacgg ccatggaaaa aaatggcgtt
    23761 aaaaatatca tctttaccag ttccgttgct gtttatggtt tgaacaaaca caaccctgac
    23821 gaaaaccatc cacacgatcc tttcaaccac tacggcaaaa gtaagtggca ggcagaggaa
    23881 gtgctgcgtg aatggtataa caaagcacca acagaacgtt cattaaccat catccgtcct
    23941 accgttatct tcggtgaacg caaccgcggt aacgtctata acttgctgaa acagatcgct
    24001 ggcggcaagt ttatgatggt gggcgcaggg actaactata agtccatggc ttatgttgga
    24061 aacattgttg agtttatcaa gtacaaactg aagaatgttg ccgcaggtta tgaggtttat
    24121 aattacgttg ataagccaga tctgaacatg aaccagttgg ttgctgaagt tgaacaaagt
    24181 ctgaacaaaa agatcccttc tatgcacttg ccttacccac taggaatgtt gggtggatat
    24241 tgctttgata tcctgagcaa aattacgggc aaaaaatacg ctgtcagctc tgtgcgcgtg
    24301 aaaaaattct gcgcaacaac acagtttgac gcaacgaaag tgcattcttc aggttttgtg
    24361 gcaccgtata cgctgtcgca aggtctggat cgaacactgc agtatgaatt cgttcatgcc
    24421 aaaaaagacg acataacgtt tgtttctgag taatgcagac gagtcagaga aaaaaggacc
    24481 aggttggact acgattattc ggaaagaggt actgattaaa ttatcagtat gcggaattcc
    24541 gagtaatccg agtaacccag atgctgatgg tgtgtagtgt tattctaagg tttaagcaaa
    24601 gttatgcgga actaagcttt ggtatatagc cataacgttt ggctaaccta tggcaataat
    24661 ttacgcacta tcagccactt agaggtagga aaagaatgac gaatttaaaa gcagttatac
    24721 cggtagcagg tcttgggatg catatgttgc ctgccactaa ggcgattccc aaagagatgc
    24781 taccgatcgt cgacaagcca atgattcagt acattgttga cgagattgtg gctgcaggga
    24841 tcaaagaaat cctcctggta actcacgcgt ccaagaacgc ggtcgaaaac cacttcgaca
    24901 cctcttatga attagaatct ctccttgagc agcgcgtgaa gcgtcaacta ctggcggaag
    24961 tgcaatctat ctgcccgccg ggcgtgacta ttatgaacgt gcgtcagggc gaacctttag
    25021 gtttaggtca ctccattttg tgtgcacgcc ccgccattgg tgacaaccca tttgtcgtgg
    25081 tgctgccgga cgttgttatc gatgatgcca gcgccgaccc gctgcgttac aaccttgccg
    25141 ctatgattgc gcgcttcaac gaaacgggcc gcagccaggt gctggcaaaa cgtatgccgg
    25201 gtgacctctc tgaatactcc gtcatccaga ccaaagaacc gctggatcgt gaaggcaaag
    25261 tcagccgcat tgttgaattc atcgaaaaac cggatcagcc gcagacgctg gactcagaca
    25321 tcatggccgt tggtcgctat gtgctttctg ccgatatttg gccggaactg gaacgcactc
    25381 agcctggtgc atgggggcgt attcagctga ctgatgctat tgccgaactg gcgaaaaaac
    25441 agtccgttga tgccatgctg atgactggtg acagctacga ctgtggtaaa aaaatgggct
    25501 atatgcaggc gtttgtgaag tatggactgc gcaacctgaa agaaggggcg aagttccgta
    25561 aaggtattga gaagctgtta agcgaataat gaaaatctga ccggatgtaa cggttgataa
    25621 gaaaattata acggcagtga agattcgtgg cgaaagtaat ttgttgcgaa tattcctgcc
    25681 gttgttttat ataaacaatc agaataacaa agagttagca ataggatttt cgtcaaagtt
    25741 ttccaggatt ttccttgttt ccagagcgga ttggtaagac aattagcgtt tgaatttttc
    25801 gagttaagcg cgagtgggta acgctcgtca catcgtaggc atgcatgcag tgctctggta
    25861 gctgtaaagc caggggcggt agcgtgcatt aatacctcta ttaatcaaac taagagccgc
    25921 ttatttcaca gcatgctctg aagtaatatg gaataaaaga gtgaagatac ttgttactgg
    25981 tggcgcagga tttattggtt ctgctgtagt tcgtcacatt ataaataata cgcaggatag
    26041 tgttgttaat gtcgataaat taacgtacgc cggaaacctg gaatcacttg ctgatgtttc
    26101 tgattctgaa cgctatattt ttgaacatgc ggatatttgc gatgctgctg caatggcgcg
    26161 gatttttgct cagcatcagc cggatgcagt gatgcacctg gctgctgaaa gccatgtgga
    26221 tcgttcaatt acaggccctg cggcatttat tgaaaccaat attgttggta cttatgtcct
    26281 tttggaagcc gctcgcaatt attggtctgc tcttgatggc gacaagaaaa atagcttccg
    26341 ttttcatcat atttctactg acgaagtcta tggtgatttg cctcatcctg acgaagtaaa
    26401 taataaagaa caattacccc tctttactga gacgacagct tacgcgccta gtagtcctta
    26461 ttccgcatca aaagcatcca gcgatcattt agtccgtgcg tggaaacgta cctatggttt
    26521 accgaccatt gtgactaatt gttcgaataa ctacggtcct tatcactttc cggaaaaatt
    26581 gattccacta gtaattctta atgctctgga aggtaaggca ttacctattt atggcaaagg
    26641 ggatcaaatt cgcgactggc tgtatgttga agatcatgca cgtgcgttat ataccgtcgt
    26701 aaccgaaggt aaagcgggtg aaacttataa cattggtgga cacaacgaaa agaaaaacat
    26761 cgatgtagtg ctcactattt gtgatttgtt ggatgagatt gtaccgaaag agaaatctta
    26821 ccgcgagcaa attacttatg ttgccgatcg tccgggacac gatcgccgtt atgccattga
    26881 tgctgagaag attggtcgcg aattgggatg gaaaccacag gaaacgtttg agagcgggat
    26941 tcggaagaca gtggaatggt acctgtccaa tacaaaatgg gttgataatg tgaaaagtgg
    27001 tgcctatcaa tcgtggattg aacagaacta tgagggccgc cagtaatgaa tattctcctt
    27061 ttcggcaaaa cagggcaggt aggttgggaa ctacagcgtg ctctggcacc tttgggtaat
    27121 ttgattgctc ttgatgttca ctccactgat tattgtggtg attttagtaa tcctgaaggt
    27181 gtggctgaaa ccgtcaaaaa aattcgccct gatgttattg ttaatgctgc ggctcatacc
    27241 gcagtagata aggctgagtc agaacccgaa tttgcacaat tactcaatgc gaccagcgtt
    27301 gaatcaattg caaaagcggc taatgaagtt ggggcctggg taattcatta ctcaactgac
    27361 tacgtatttc cgggaaccgg tgaaatacca tggctggaga cggatgcaac cgcaccgcta
    27421 aatgtttacg gtgaaaccaa gttagctgga gaaaaagcat tacaagagca gtgtgcgaag
    27481 caccttattt tccgtaccag ctgggtatat gcaggtaaag gaaacaattt tgccaagaca
    27541 atgttacgtc tggcaaaaga gcgtgaagaa ttagccgtta ttaatgatca gtttggtgcg
    27601 ccaactggcg cagagttgct ggctgattgt acggcacatg ccattcgtgt ggcactgaat
    27661 aaaccggaag tcgcaggttt gtaccatctg gtagccagtg gtaccacaac ctggcacgat
    27721 tatgctgcgc tggtttttga agaggcgcgc aaagcaggca taccccttgc actcaacaag
    27781 ctcaacgcag tagcaacaac agcctatcct acaccagctc gtcgtccaca taactctcgc
    27841 cttaatacag aaaaatttca gcagaatttt gcgcttgttt tgcctgactg gcaggttggc
    27901 gtgaaacgaa tgctcaacga attatttacg actacagtaa tttaatagtt tttgcatctt
    27961 gttcgtgatg atggagcaag atgaattaaa aggaatgatg aaatgaaaac gcgtaaaggt
    28021 attattttag cgggtggctc tggtactcgt ctttatcctg tgactatggc tgtcagtaaa
    28081 cagctgttac ctatttatga taaaccgatg atctattacc cgctttctac actgatgtta
    28141 gcgggtattc gcgatattct gattatcagt acaccacagg atactcctcg ttttcaccaa
    28201 ctgctgggtg acgggggcca gtgggggcta aatcttcagt acaaagtgca accgactcca
    28261 gatgggcttg cgcaggcgtt tattatcggt gaagagttta tcggtggtga tgattgtgct
    28321 ctggttctcg gtgataatat cttttacggt catgatctgc cgaagttaat ggatgtcgct
    28381 gttaacaaag aaagtggtgc aacagtattt gcctatcatg ttaatgatcc tgaacgctac
    28441 ggtgtcgttg agtttgataa aaaaggtacg gcaattagcc tggaagaaaa accgttacaa
    28501 ccaaaaagta attatgcggt aaccgggctt tatttctatg ataacgacgt tgtcgaaatg
    28561 gcgaaaaacc ttaagccttc tgcccgtggt gaactggaaa ttaccgatat taaccgtatt
    28621 tatatggaac agggggggtt atccgttgcc atgatgggac gtggttatgc atggctggac
    28681 acgggtacac atcaaagtct tattgaagca agcaacttca ttgcaacaat tgaagagcgt
    28741 caggggttaa aagtttcctg tccggaagaa attgcttacc gtaaagggtt tattgatgct
    28801 gagcaggtga aagtattagc tgaaccgctg aaaaaaaatg cttatggtca gtatctgctg
    28861 agaatgatta aaggttatta ataaaatgaa cgtaattaaa acagaaattc cagaagtctt
    28921 gatttttgag cctaaaaaat ttgaggactc cagggggtat ttttttgaaa gctttaatca
    28981 aaggcttttt gaagatgccg taggaaaaga aattctattc gtccaagata atcaatcata
    29041 ttcaagtaaa aatgtattaa gaggtttaca ttatcaaagc gagccgtatg ctcaagggaa
    29101 actagtaaga tgtattatgg gggaagtatt tgatgtagct gtagatatta gaaaagagtc
    29161 agaaagttat gggcaatggg tt
//
LOCUS       DAZGAP010000044        29089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGAP010000044 DAZGAP010000000
VERSION     DAZGAP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.044"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(135..1610)
                     /gene="ampG"
                     /locus_tag="ACHLAM_001619"
     CDS             complement(135..1610)
                     /gene="ampG"
                     /locus_tag="ACHLAM_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO7853169.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1654..2232)
                     /gene="yajG"
                     /locus_tag="ACHLAM_001620"
     CDS             complement(1654..2232)
                     /gene="yajG"
                     /locus_tag="ACHLAM_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7853170.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2537..2854
                     /gene="bolA"
                     /locus_tag="ACHLAM_001621"
     CDS             2537..2854
                     /gene="bolA"
                     /locus_tag="ACHLAM_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO7853171.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3198..4496
                     /gene="tig"
                     /locus_tag="ACHLAM_001622"
     CDS             3198..4496
                     /gene="tig"
                     /locus_tag="ACHLAM_001622"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO7853172.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4742..5365
                     /gene="clpP"
                     /locus_tag="ACHLAM_001623"
     CDS             4742..5365
                     /gene="clpP"
                     /locus_tag="ACHLAM_001623"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO7853173.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5491..6765
                     /gene="clpX"
                     /locus_tag="ACHLAM_001624"
     CDS             5491..6765
                     /gene="clpX"
                     /locus_tag="ACHLAM_001624"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO7853174.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6953..9307
                     /gene="lon"
                     /locus_tag="ACHLAM_001625"
     CDS             6953..9307
                     /gene="lon"
                     /locus_tag="ACHLAM_001625"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO7853175.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9498..9788
                     /gene="hupB"
                     /locus_tag="ACHLAM_001626"
     CDS             9498..9788
                     /gene="hupB"
                     /locus_tag="ACHLAM_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO7853176.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9980..11851
                     /gene="ppiD"
                     /locus_tag="ACHLAM_001627"
     CDS             9980..11851
                     /gene="ppiD"
                     /locus_tag="ACHLAM_001627"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7853177.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVVQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKK
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12002..12373
                     /gene="ybaV"
                     /locus_tag="ACHLAM_001628"
     CDS             12002..12373
                     /gene="ybaV"
                     /locus_tag="ACHLAM_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO7853178.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12467..12865
                     /gene="fadM"
                     /locus_tag="ACHLAM_001629"
     CDS             12467..12865
                     /gene="fadM"
                     /locus_tag="ACHLAM_001629"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO7853179.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12917..13612)
                     /gene="queC"
                     /locus_tag="ACHLAM_001630"
     CDS             complement(12917..13612)
                     /gene="queC"
                     /locus_tag="ACHLAM_001630"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO7853180.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLHHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13677..15377)
                     /gene="ybaE"
                     /locus_tag="ACHLAM_001631"
     CDS             complement(13677..15377)
                     /gene="ybaE"
                     /locus_tag="ACHLAM_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO7853181.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLLLLQLPARDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15477..16295
                     /gene="cof"
                     /locus_tag="ACHLAM_001632"
     CDS             15477..16295
                     /gene="cof"
                     /locus_tag="ACHLAM_001632"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO7853182.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGNVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16448..16906
                     /gene="decR"
                     /locus_tag="ACHLAM_001633"
     CDS             16448..16906
                     /gene="decR"
                     /locus_tag="ACHLAM_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO7853183.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16936..18708
                     /gene="mdlA"
                     /locus_tag="ACHLAM_001634"
     CDS             16936..18708
                     /gene="mdlA"
                     /locus_tag="ACHLAM_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7853184.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            18701..20482
                     /gene="mdlB"
                     /locus_tag="ACHLAM_001635"
     CDS             18701..20482
                     /gene="mdlB"
                     /locus_tag="ACHLAM_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7853185.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20663..21001
                     /gene="glnK"
                     /locus_tag="ACHLAM_001636"
     CDS             20663..21001
                     /gene="glnK"
                     /locus_tag="ACHLAM_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO7853186.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21031..22317
                     /gene="amtB"
                     /locus_tag="ACHLAM_001637"
     CDS             21031..22317
                     /gene="amtB"
                     /locus_tag="ACHLAM_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO7853187.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22366..23226)
                     /gene="tesB"
                     /locus_tag="ACHLAM_001638"
     CDS             complement(22366..23226)
                     /gene="tesB"
                     /locus_tag="ACHLAM_001638"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO7853188.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23444..24013
                     /gene="ybaY"
                     /locus_tag="ACHLAM_001639"
     CDS             23444..24013
                     /gene="ybaY"
                     /locus_tag="ACHLAM_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO7853189.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQPAI
                     QQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVL
                     PFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQ
                     ASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24044..24355)
                     /locus_tag="ACHLAM_001640"
     CDS             complement(24044..24355)
                     /locus_tag="ACHLAM_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO7853190.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24518..24614
                     /gene="ffs"
                     /locus_tag="ACHLAM_001641"
     ncRNA           24518..24614
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHLAM_001641"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24734..25087
                     /gene="ybaA"
                     /locus_tag="ACHLAM_001642"
     CDS             24734..25087
                     /gene="ybaA"
                     /locus_tag="ACHLAM_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO7853191.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25129..26679)
                     /gene="pdeB"
                     /locus_tag="ACHLAM_001643"
     CDS             complement(25129..26679)
                     /gene="pdeB"
                     /locus_tag="ACHLAM_001643"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO7853192.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26843..27313)
                     /gene="ylaC"
                     /locus_tag="ACHLAM_001644"
     CDS             complement(26843..27313)
                     /gene="ylaC"
                     /locus_tag="ACHLAM_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO7853193.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27429..27980)
                     /gene="maa"
                     /locus_tag="ACHLAM_001645"
     CDS             complement(27429..27980)
                     /gene="maa"
                     /locus_tag="ACHLAM_001645"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO7853194.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28153..28371)
                     /gene="hha"
                     /locus_tag="ACHLAM_001646"
     CDS             complement(28153..28371)
                     /gene="hha"
                     /locus_tag="ACHLAM_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO7853195.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28397..28771)
                     /gene="tomB"
                     /locus_tag="ACHLAM_001647"
     CDS             complement(28397..28771)
                     /gene="tomB"
                     /locus_tag="ACHLAM_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO7853196.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7307 a         6978 c         7843 g         6961 t
ORIGIN      
        1 gaattcataa atggcgtcaa taatttccag acgctaagcc gcacaaaaga acatatatta
       61 ataagcgatg ctgatgttta tttagccgta ataattacgg cgagtgatta ctacggctaa
      121 ataatattta cagattacgt cagatgcgtt tttcgtagcg ccagataatc cagtaaacca
      181 ccaagcacga cgccagaaag ggcgactaaa accccgactt ccagcagagc aggcaggaaa
      241 gagaagtgcg tcaaatccag cgcgtccatc gtcaacagca gtaaccacac ggccaacagg
      301 ctgacgcccg ccgccagtgt ccacatggca aaggcataac ctgccggata ttcggtacgg
      361 gagataaagt tatcatttac tcgtgtatat tcaagcgtct ggcgacaaac cagcagcaag
      421 ataagccctg gtacggcagc ggcgacggag aatagataga atgtcgacca gccgtgtgct
      481 tcaacaaacc aacccgccac ggggccgaca taaactcgcc ctacagcaga aagcgctgag
      541 agcagggcaa attgagtagc ggaaaatgac ttattacaca gcgtcattaa cagcgcgaca
      601 aaggctgatg tacccatccc gccacagagg ttttcgaaaa agacggctgc gcccatgctg
      661 tagagatgct tatcagtaat cgacagcagc cagtaaccgg cgttagatgc accttgtaaa
      721 atgccgaaaa tcagcagtgc acggaacagc gacaggcgct gcatcaaaat cccaccgtac
      781 aatgcgccaa caatggtcgc cagtaagcca agcgttttgt taaccacgcc tacttcaccc
      841 gcatcaaacc cgacgccgcg aatcaaaaac gtggttgtca ggctcatagc gaatgcgtcg
      901 cccagcttat acagcacgat aagaagcaaa ataagccagg cattattgcg accaaagaaa
      961 tctcgcagag gtgcaacaac cgcttgttcc agcgttttgg gcacaggaat ggtgtcggtg
     1021 ggttctggtg caagcaacgt cgcgataata caggggatca acagcgccgc catcaaccag
     1081 tacatgccct gccagcccag ccatttatct gccagccaca gggctaagcc gccggaaacc
     1141 agcatcccta aacggtaacc cagcacgctg attgccgcgc ccgcaccacg ttcttctgcc
     1201 gggagcacat cggttttcca cgcatcgaag acaatatcct gggaggcaga gcaaaaagcg
     1261 atcaccaccg ccagtgccgc catccagcgg agctgggtgc ctggttcgag aaaacccatc
     1321 gccgcaatgg cgactaataa cagaatttgc gtggcgagca gccaaccgcg ccgccgcccg
     1381 aaaaatgggg gcgtgtagcg gtccatcagt ggtgaccaga ggaatttaaa aacgtaagcc
     1441 tggcctacca gagagaagaa accaatggtt ttgagatcga tattctcgac cgtcatccag
     1501 gcctgtaagg tgccggacgt cagggcgagc ggtagcccgg aagcaaaacc caggatcagc
     1561 aatatggctg aacgcggctg ttgaaaaata cgtaaatatt gactggacat gggcgtctac
     1621 aggcccggct tgcaccgggc cagagggcag aaattaacgc gcgttctgct tgatgaattc
     1681 gtggatgctg gtgtcctgag acatatcagc gatggtatcg gtcagcacac tgttaaccgc
     1741 atcggcgata tttttgttgg aggcctggaa cgcaccttca acgttgtagc tggcacgata
     1801 gtttttggtc attttgttgc cattctgcgc ggtagcgatg atggcgatat ccgctttggt
     1861 cgcgatgttg tagcgcacgt tgccctggga cacgtcagca tacagttggc taacgatgat
     1921 ttgcagatta accgggccat tcggaccaac catgtatcca cgggcggtca tttgtttttc
     1981 cagcacttct tgcagcagga aacgcagatc gcgggaggcg gtcagggtaa cgatttgatt
     2041 atcgcgggtg acttttgcca gcgcctgatc ggtacgctga tcggcaccat taatgcttac
     2101 ggtgacgccc atcaggcttg gatcctgctg tggcagtgta atcgtcgggg aaacttcaat
     2161 agttgttggc ggttttgcgc atcctgcaag cataaacaga gcaactaacg ggaagaggat
     2221 ttttttgaac atgttcgggc tctcagagac tcttaagcgt gtttggtaaa aattcccgcc
     2281 atcataacat tgccaacggc gaggggaagt gggtaaggca tgtaaattca tcatgttgac
     2341 gaaataatcg cccctggtaa aagaaacgct gatgcgaggc ctgtgtttca atctttaaat
     2401 cagtaaactt catacgcttg acagaaaaac caggacgaaa cctaaatatt tgttgttaag
     2461 ctgcaatgga aacggtaaaa gcggctagta tttaaaggga tggatgacat ctcagcgttg
     2521 tcggaggaga tatttcatga tgatacgtga gcggatagaa gaaaaattaa gggcggcgtt
     2581 ccaacccgta ttcctcgaag tagtggatga aagctatcgt cacaatgtcc cagccggctc
     2641 tgaaagccat tttaaagttg tgctggtcag cgatcgtttt acgggtgaac gttttctgaa
     2701 tcgtcatcga atgatttaca gtactttagc ggaggaactc tctactaccg ttcatgcgct
     2761 ggctctgcat acttacacta ttaaggagtg ggaagggttg caggacaccg tctttgcctc
     2821 tcctccctgt cgtggagcag gaagcatcgc gtaaaaacgc atttgcaact gtcggcgctt
     2881 ttccagtatg ttgctaaaga ttttatgaaa aacggcctgc gggccgtttt gttttgtctg
     2941 gattttgcgc tttttgccca gcattcagac gaaaattgcc cgggaattgt gaaaaaatac
     3001 gcgacagcgc gcaataaccg ttctcgactc ataaaagtga tgccgctata atgccgcgtc
     3061 ctatttgaat gctttcggga tgattctggt aacagggaat gtgattgatt ataagaacat
     3121 cccggttccg cgaagccaac aacctgtgct tgcggggtaa gagttgaccg agcactgtga
     3181 ttttttgagg taacaagatg caagtttcag ttgaaaccac tcaaggcctt ggccgccgtg
     3241 taacgattac tatcgctgct gacagcatcg agaccgctgt taaaagcgag ctggtcaacg
     3301 ttgcgaaaaa agtacgtatt gacggcttcc gcaaaggcaa agtgccaatg aatatcgttg
     3361 ctcagcgtta tggtgcgtct gtacgccagg acgttctggg tgacctgatg agccgtaact
     3421 tcattgacgc catcattaaa gaaaaaatca atccggctgg cgcaccgact tatgttccgg
     3481 gcgaatacaa gctgggtgaa gacttcacct actctgtaga gtttgaagtt tatccggaag
     3541 ttgaactgca gggtctggaa gcgatcgaag ttgaaaaacc gatcgttgaa gtgaccgacg
     3601 ctgacgttga cggcatgctg gatactctgc gtaaacagca ggcgacctgg aaagaaaaag
     3661 acggcgctgt tgaagcagaa gaccgcgtaa ccatcgactt caccggttct gtagacggcg
     3721 aagagttcga aggcggtaaa gcgtctgatt tcgtactggc gatgggccag gggcgtatga
     3781 ttccgggctt tgaagacggt atcaaaggcc acaaagctgg cgaagagttc accatcgacg
     3841 tgaccttccc ggaagaatac cacgcagaaa acctgaaagg taaagcagcg aaattcgcta
     3901 tcaacctgaa gaaagttgaa gagcgtgaac tgccggaact gactgcagaa ttcatcaaac
     3961 gtttcggcgt tgaagatggt tccgtagaag gtctgcgcgc tgaagtgcgt aaaaacatgg
     4021 agcgcgagct gaagagcgcc atccgtaacc gcgttaagtc tcaggcgatc gaaggtctgg
     4081 taaaagctaa cgacatcgac gtaccggctg cgctgatcga cagcgaaatc gacgttctgc
     4141 gtcgccaggc tgcacagcgt ttcggtggca acgaaaaaca agctctggaa ctgccgcgtg
     4201 aactgtttga agaacaggct aaacgtcgcg tagttgttgg cctgctgctg ggcgaagtta
     4261 tccgcaccaa cgagctgaaa gctgacgaag agcgcgtgaa aggcctgatc gaagagatgg
     4321 cttctgcgta cgaagatccg aaagaagtta tcgagttcta cagcaaaaac aaagaactga
     4381 tggacaacat gcgcaatgtt gctctggaag aacaggctgt tgaagctgta ctggcgaaag
     4441 cgaaagtgac tgaaaaagaa accactttca acgagctgat gaaccagcag gcgtaattta
     4501 cgcagcataa cgcgctaaat tcgcacaaag gcccgtcacc gccaggtggt gggctttttt
     4561 ttgtcatgaa ttttgcatgg aaccgtgcga aaagcctctt tcggtgttag cgtaacaaca
     4621 aaagattgtt atgcttgaaa tatggtgatg ccgtacccat aacacaggga ctagctgata
     4681 atccgtccat aaggttacaa tcggtacagc aggttttttc aattttatcc aggagacgga
     4741 aatgtcatac agcggcgaac gagataactt tgcaccccat atggcgctgg tgccgatggt
     4801 cattgaacag acctcacgtg gtgagcgctc ttttgatatc tattctcgtc tacttaagga
     4861 acgcgtcatt tttctgactg gccaggttga agaccacatg gctaacctga ttgtggcgca
     4921 gatgctgttc ctggaagcag aaaacccaga aaaagatatc tatctgtaca ttaactcccc
     4981 aggcggggtg atcactgccg ggatgtctat ctatgacacc atgcagttta tcaagcctga
     5041 tgtcagcacc atctgtatgg gccaggcggc ttcgatgggc gctttcttgc tgactgcagg
     5101 ggcaaaaggt aaacgtttct gcctgccgaa ttcgcgcgtg atgattcacc agccgttggg
     5161 cggctaccag ggccaggcga ccgatatcga aattcatgcc cgtgaaattc tgaaagttaa
     5221 agggcgcatg aatgaactta tggcgcttca tacgggtcaa tcattagaac agattgaacg
     5281 tgataccgag cgcgatcgct tcctttccgc ccctgaagcg gtggaatacg gtctggtcga
     5341 ttcgattctg acccatcgta attgatgcca gaggcgcaac tgtgccgcta tacttatcca
     5401 gggcggcaca acgctgtaag cggcttgcgc ctgagaatgg catttgcgtc gtcgtgtgcg
     5461 gcacaaagaa caaagaagag gttttgaccc atgacagata aacgcaaaga tggctcaggc
     5521 aaattgctgt attgctcttt ttgcggcaaa agccagcatg aagtgcgcaa gctgattgcc
     5581 ggtccatccg tgtatatctg cgacgaatgt gttgatttat gtaacgacat cattcgcgaa
     5641 gagattaaag aagttgcacc gcatcgtgaa cgcagtgcgc taccgacgcc gcatgaaatt
     5701 cgcaaccacc tggacgatta cgttatcggc caggaacagg cgaaaaaagt gctggcggtc
     5761 gcggtataca accactacaa acgtctgcgc aacggcgata ccagcaatgg tgtcgagttg
     5821 ggcaaaagta acattctgct gatcggtccg accggttccg gtaaaacgct gctggctgaa
     5881 acgctggcgc gcctgctgga cgtcccgttc accatggccg acgcaaccac gctgaccgaa
     5941 gccggttatg tgggcgaaga cgttgaaaac atcattcaga agctgttgca gaagtgcgat
     6001 tacgacgtac agaaagcgca gcgcgggatt gtctacatcg atgaaatcga caagatttct
     6061 cgtaagtcag acaacccgtc cattacccga gacgtttccg gtgaaggcgt acagcaggca
     6121 ctgttgaaac tgatcgaagg tacggtagct gctgttccac cgcaaggtgg gcgtaaacat
     6181 ccgcagcagg aattcttgca ggttgatacc tctaagatcc tgtttatttg tggcggtgcg
     6241 tttgccggtc tggataaagt gatttcccat cgtgtagaaa ccggctccgg cattggtttt
     6301 ggcgcgacgg taaaagcgaa gtccgacaaa gcaagcgaag gcgagctgct ggcgcaggtt
     6361 gaaccggaag atctgatcaa gtttggtctt atccctgagt ttattggtcg tctgccggtt
     6421 gtcgcaacgt tgaatgaact gagcgaagaa gctctgattc agatcctcaa agagccgaaa
     6481 aacgccctga ccaagcagta tcaggcgctg tttaatctgg aaggcgtgga tctggaattc
     6541 cgtgacgagg cgctggatgc tatcgctaag aaagcgatgg cgcgtaaaac cggtgcccgt
     6601 ggcctgcgtt ccatcgtaga agccgcactg ctcgatacca tgtacgatct gccgtccatg
     6661 gaagacgtcg aaaaagtggt tatcgacgaa tcggtaattg atggtcaaag caagccgttg
     6721 ctgatttatg gcaagccgga agcgcaacag gcatctggtg aataattaac cattcccata
     6781 caattagtta accaaaaagg ggggatttta tctccccttt aatttttcct ctattctcgg
     6841 cgttgaatgt gggggaaaca tccccatata ctgacgtaca tgttaataga tggcgtgaag
     6901 cacagtcgtg tcatctgatt acctggcgga aattaaacta agagagagct ctatgaatcc
     6961 tgagcgttct gaacgcattg aaatccccgt attgccgctg cgcgatgtgg tggtttatcc
     7021 gcacatggtc atccccttat ttgtcgggcg ggaaaaatct atccgttgtc tggaagcggc
     7081 gatggaccat gataaaaaaa ttatgctggt cgcgcagaaa gaagcttcaa cggatgagcc
     7141 gggtgtaaac gatcttttca ccgtcgggac cgtggcctct atattgcaga tgctgaaact
     7201 gcctgacggc accgtcaaag tgctggtcga ggggttacag cgcgcgcgta tttctgcgct
     7261 ctctgacaat ggcgaacact tttctgcgaa ggcggagtat ctggagtcgc cgaccattga
     7321 tgagcgggaa caggaagtgc tggtgcgtac tgcaatcagc cagttcgaag gctacatcaa
     7381 gctgaacaaa aaaatcccac cagaagtgct gacgtcgctg aatagcatcg acgacccggc
     7441 gcgtctggcg gataccattg ctgcacatat gccgctgaaa ctggctgaca aacagtccgt
     7501 tctggagatg tccgacgtta acgaacgtct ggaatatctg atggcaatga tggaatcgga
     7561 aatcgatctg ctgcaggttg agaaacgcat tcgcaaccgc gttaaaaagc agatggagaa
     7621 atcccagcgt gagtactatc tgaacgagca aatgaaagct attcagaaag aactcggtga
     7681 aatggacgac gcgccggacg aaaacgaagc cctgaagcgc aaaattgacg cggcgaaaat
     7741 gccgaaagag gcaaaagaga aagcggaagc agagttgcag aagctgaaaa tgatgtctcc
     7801 gatgtcggca gaagcgaccg tagtgcgtgg ttatatcgac tggatggtac aggtgccgtg
     7861 gaatgcgcgc agcaaggtca aaaaagacct gcgtcaggcg caggaaatcc ttgataccga
     7921 ccattatggt ctggagcgcg tgaaagatcg catccttgag tatcttgcgg ttcaaagccg
     7981 tgtcaacaaa atcaagggac cgatcctctg cctggtaggg ccgccggggg taggtaaaac
     8041 ctctcttggt cagtccattg ccaaagccac cgggcgtaaa tatgtccgta tggcgctggg
     8101 cggcgtgcgt gatgaagcgg aaatccgtgg tcaccgccgt acttacatcg gttctatgcc
     8161 gggtaaactg atccagaaaa tggcgaaagt gggcgtgaaa aacccgctgt tcctgctcga
     8221 tgagatcgac aaaatgtctt ctgacatgcg tggcgatccg gcctccgcac tgcttgaagt
     8281 gctggatcca gagcagaacg tagcgttcag cgaccactac ctggaagtgg attacgacct
     8341 tagcgacgtg atgtttgtcg cgacgtcgaa ctccatgaac attccggcac cgctgctgga
     8401 tcgtatggaa gtgattcgcc tctccggtta taccgaagat gaaaaactga acatcgccaa
     8461 acgtcacctg ctgccgaagc agattgaacg taatgcactg aaaaaaggtg agctgaccgt
     8521 cgacgatagc gccattatcg gcattattcg ttactacacc cgtgaggcgg gcgtgcgtgg
     8581 tctggagcgt gaaatctcca aactgtgtcg caaagcggtt aagcagttac tgctcgataa
     8641 gtcattaaaa catatcgaaa ttaacggcga taacctgcat gactatctcg gtgttcagcg
     8701 cttcgactat ggtcgcgcgg ataacgaaaa ccgtgtcggt caggtaactg gtctggcgtg
     8761 gacggaagtg ggcggtgact tgctgaccat tgaaaccgcg tgcgttccgg gtaaaggcaa
     8821 actgacctat accggatcgc ttggcgaagt gatgcaggag tccattcagg cggcgttaac
     8881 ggtggttcgt gcgcgtgcgg aaaaactggg gatcaaccct gatttttatg aaaaacgcga
     8941 catccacgtc cacgtaccgg aaggtgcgac gccgaaagat ggtccgagtg ccggtattgc
     9001 tatgtgcacc gcgctggttt cttgcctgac cggtaacccg gttcgtgctg atgtggcaat
     9061 gaccggtgag atcactctgc gtggtcaggt actgcctatc ggtggtttga aagaaaaact
     9121 actggcagcg catcgcggcg ggatcaaaac agtgttaatt ccgttcgaaa ataaacgcga
     9181 tctggaagag attcctgaca acgtaattgc cgatctggat attcatcctg tgaaacgcat
     9241 tgaggaagtt ctgactctgg cgctgcaaaa tgaaccgtct ggcatgcagg ttgtgactgc
     9301 aaaatagtga cctcgcgcaa aatgcactaa taaaaacagg gctggcaggc taattcggac
     9361 ttgccagcct ttttttgtct cgctaagtta gatggcggat tgggcttgcc cttattaagg
     9421 ggtgttgtaa ggggatggct ggcctgatat aactgctgcg cgttcgtacc ttgaaggatt
     9481 caagtgcgat ataaattata aagaggaaga gaagagtgaa taaatctcaa ttgatcgaca
     9541 agattgctgc aggggctgat atctctaaag ctgcggctgg ccgtgcgtta gatgctatta
     9601 ttgcttccgt aactgaatct ctgaaagaag gggatgatgt agcactggta ggttttggta
     9661 cttttgccgt taaagagcgt gctgcccgta ctggccgcaa tccgcagacc ggtaaagaga
     9721 tcaccatcgc tgctgctaaa gtaccgagct tccgtgcagg taaagcactg aaagacgcgg
     9781 taaactaagc gttgtcccca gtggggatgt gacgaagttc aagggcgcat ctactgatgt
     9841 gcctttttta tttgtattcg gtgactttct gcgtcttgtg ggctgacaat tgcccccgtt
     9901 tcttgtcaca atatgccttt gcgcgcatcg atacgttgcg tgaggtacac agtcatctac
     9961 agcggagtgt tgttacacca tgatggacag cttacgcacg gctgcaaaca gtctcgtgct
    10021 caagattatt ttcggtatca ttatcgtgtc gttcatattg accggcgtga gtggttacct
    10081 gattggcgga ggcaataact acgccgcaaa agtgaatgac caggaaatca gtcgtgggca
    10141 attcgagaac gccttcaaca gcgagcgtaa tcgcatgcag caacagctgg gcgatcaata
    10201 ctccgagctg gcagcgaacg aaggctatat gaaaaccctg cgtcaacagg tgctgaatcg
    10261 tctgatcgac gaggcgctgc tggatcagta cgctcgtgag ctgaaactgg gtatcagcga
    10321 tgagcaggtt aaacaggcga ttttcgcgac cccagccttc caggttgacg gcaaatttga
    10381 taacagccgc tataacggta tcctcaacca gatggggatg accgccgatc agtacgccca
    10441 ggcgctgcgt aaccagctca ctacccaaca gctgattaac ggcgttgccg gtaccgattt
    10501 tatgctgaaa ggtgaaaccg acgagctggc ggcactggta gttcaacaac gcgtggtgcg
    10561 tgaagcgact atcgatgtta acgcgctggc ggcgaagcag cctgtgaccg aacaggaaat
    10621 tgccagctac tacgaacaaa acaaaaacaa tttcatgacg ccggaacaat tccgcgtgag
    10681 ttacatcaag ctggatgccg caacgatgca gcaaccggtt agcgatgcgg atatccagag
    10741 ctactacgat cagcatcagg atcaattcac ccagccgcag cgtacccgtt acagcatcat
    10801 ccagaccaaa actgaagatg aagcgaaagc ggtacttgat gagctgaata agggcggtga
    10861 ttttgctgca ttagccaaag aaaaatctgc cgatattatc tctgcccgca acggcggtga
    10921 tatgggttgg ttagaagatg ccactatccc ggacgaactg aaaaatgctg gtctgaaaaa
    10981 aaaaggccaa ctgtctggtg ttatcaaatc ttcggtcggt ttcctgattg tgcgtctgga
    11041 cgacattcag ccagcgaaag tgaaatcgtt agacgaagta cgtgacgaca ttgcggcgaa
    11101 agtgaaacac gaaaaagccc tcgatgcgta ctacgcgctg cagcagaaag tgagcgatgc
    11161 ggcaagcaac gacaccgagt ctctggccgg tgcagagcaa gctgccggcg ttaaagccac
    11221 tcagacgggt tggttcagca aagataacct gccggaagag ttgaacttca agccagttgc
    11281 cgacgctatc ttcaacggcg gtctggtcgg tgaaaacggc gcgccgggca tcaactctga
    11341 catcatcacc gtagacggcg accgcgcatt cgtgctgcgc atcagcgagc acaaaccgga
    11401 agcggtgaaa ccgttggcag atgttcagga acaagttaag gcactggttc agcacaacaa
    11461 agctgaacaa caggcgaaag tggatgctga gaaactgctg gttgatttga aagcaggcaa
    11521 aggtgcggaa gctatgcagg ctgccggtct gaaatttggc gagccgaaaa ccttaagtcg
    11581 ttccggtcgt gacccgatta gccaggctgc gtttgcactg ccgctgccag cgaaagacaa
    11641 accgagctac ggtatggcga ccgatatgca aggtaatgta gttctgctgg cgctggatga
    11701 agtgaagcaa ggttcaatgc cggaagatca gaaaaaagcg atggtgcagg gtatcaccca
    11761 gaacaacgca caaatcgtct ttgaagctct gatgagtaac ctgcgtaaag aggcgaaaat
    11821 caaaattggc gatgcgctgg aacagcaata atcctgaagc cgcctcgcaa aaaaatgctt
    11881 tgcagctgta acaagaaaag gtcgctttcg cggccttttc catttctgaa cattgccatt
    11941 tgtttactgt tttcactgcc gttaaggtga ttccactgtt aacaaacaag gagaaaacag
    12001 tatgaaacac ggaattaaag cactgctcat taccctgtcc ctggcctgtg ccggaatgtc
    12061 tcatagtgct ctggcggcag ctcctgcggc aaaaccgaca acggtagaaa ccaaagcgga
    12121 agctcctgca gcacaaagta aagcagcagt tccggcgaaa gccagtgacg aagaaggcac
    12181 ccgggtcagc attaataatg ccagcgcgga agagttagcc cgcgcgatga atggcgttgg
    12241 cctgaagaaa gcgcaggcga ttgtcagtta tcgcgaagag tacggtccgt ttaaaacggt
    12301 ggaggatcta aagcaggtgc cggggatggg caattcgctg gtggagcgta atctggcggt
    12361 attaaccctg taattaattt gcatagtggc aattttttgc cagactgaag aggtcatacc
    12421 agttatgacc tctgtactta taacaacaac gtaaggttct cgcgctatgc aaacacaaat
    12481 caaagttcgt ggatatcatc tcgatgttta ccagcacgtt aataacgccc gctatctgga
    12541 gtttctcgaa gaagcccgct gggatgggtt ggaaaatagc gacagttttc agtggatgac
    12601 ggcccataac atcgctttcg tggtggtgaa tatcaatatt aactatcgtc gcccggcggt
    12661 attaagcgac ttgttaacca ttacgagcca gttacagcaa ttaaacggta aaagcggcat
    12721 cttaagccag gtcattacac tggagccgga agggcaggtg gtagcggatg cgcttattac
    12781 gtttgtttgt attgatctta aaacgcagaa agcattagct ctggaagggg aattgcgcga
    12841 aaagctcgag cagatggtta agtaaacgtt ttgtggtgcc ggatgctcaa gccgcatccg
    12901 gcgacacccg gaataattac ttcaacccgg ttttctgctt catcgctgcc atcaccgtcg
    12961 gtttatcggc cagataatga tgcaaaccgt tggcacgtaa attacacgcc gcacaatgac
    13021 cgcaaccgtc gcctttaatg ccgttataac aggtcagcgt ttcgttacgg actaaatcca
    13081 gcttgccgta ataatctgcc agcgcccagg tttccgcttt atcaatccac atcagcggcg
    13141 tttcaaaacg aatgtctttc gccataccca gactgacggc atggttcagt gctttcacaa
    13201 actcatcgcg gcaatccggg tagccggaga aatccgtttc gcagacgccg gtaatcaccg
    13261 cttctgcttt tacctgatac gcatatattg ccgccagtgt caggaacaaa atattacgcc
    13321 cagggacaaa cgtattcggg atgccatcgg cttcaggttc ataatctggc actggaatgc
    13381 tgtcacgtgt caggctgctg accgccagct cgttgagcag cgttacatca agaaccttat
    13441 gcgcgcgtgc ccccagtttc agcgccagtt cgcgtgccac gtcgatttcc gcgcgatgtc
    13501 gctgaccgta atcgaacgtc acgcaatgga cttcatcata ttgttgtaat gcctgcacca
    13561 gacaggtggt agaatcctgg cctccactga acacaacgac agcacgtttc atagataatc
    13621 ctgcttgaac aataaaagcg ttatggtaac gcctgcgatg aacccggacc agctattcat
    13681 tgcgacggcg cgggaagcca ggcttcggta aattcaaacc agcctcgcgg tgtcagtcgc
    13741 acaccgttca cgccgggagg ggcgctaatg cgatagtgat agttgaacag cggcgtcagc
    13801 gtcgcatctg ccattaactg gctaaaaacc gctttcaggg cattaaatcg gttttcctca
    13861 tcaggcatta cttgcaccgc gtccagtgtc gattgcaaat gtgcatatgc tggagcgtcg
    13921 aaaacatgtg gccatagtgg atcgcaacgc agccattgct ccagggtata ttccggtgct
    13981 tcgccaatta atctgtcgcc catcatgagg tctgcgtgtg ccagtagggt cgtgtcgtcc
    14041 cagttttttg cgttatgaaa aataattgtg agttcacagc cttccgctgc cagtgtcgcc
    14101 tgtagacgtt ctgccatggt atgaagttct atcggtaggt gataaaccag cgtcaaggtt
    14161 ttcggtagtt tgacctcatc cggtacttgc cattgcggaa tagtccagcc tggcagtaat
    14221 gcatgactgg cggtgatcag gttttctccg acttctaacg tttgtaataa accggattga
    14281 tgaataatgg aaatcacttt tcgcgcctgc cagagggaca gtcggggact tttgcgcaac
    14341 gttaaatagc aaaaacctaa actgatgcca ctactgacct ggctgacccg ttgcagctcc
    14401 tccggtttgc cgatggtgat ttgcacggga tgccgacaac tggttcccaa atctttttcg
    14461 aaaagcggcg gagttatcca gtactcaacc gctttaagca gcggatgacg taaatggtaa
    14521 taatcatggc tttccaggcg caccagctcc gctgtgaatt gtgttaagcg aaaaggaccc
    14581 gtgccgatca gtgggaattg cggatgcgcc agatggctgc aatagctcgc cagtcggtgc
    14641 gcaagccagt aatcggggcg atgtaaaaag aaggtcagac actgcggatg ggtgacttca
    14701 atacgcttca cgctaataaa taattgatcc cgtgctggca gttgtaacag cagcaataat
    14761 cgctggtgta agtgtgaggc ttttactgca tcgccgttat gccagtgtag ggttgaacga
    14821 agataaaagt cccagcgtaa cccgtcagta gaggtttccc agtgatgtgc taaatcgcca
    14881 atcgggcgct gagtattatt atcgaagcgg gtcaggccgg aaaatatctg cccggccaga
    14941 tgctgctcgg cacggccggg caaaaagccc ggttgtagcg gttcgagcgg gcgatagtag
    15001 ggaatacgca acgtgggcgt atcgttttgc cattgtccgc ccataaacgg ctgtaacaga
    15061 gtgcgcagct cacctggggc cagttgcgcc agctccagca catcttgctg ctttccggtt
    15121 tccagcgcct gttccatcat cgcattgcgt agcgattctg gcgtgaccag aaagcgtagt
    15181 tgcccgcgtt ttccgcgtcc tgactgcgcc tgccactcca gccatcccgc ctcctgtgcc
    15241 tggcgtaaca gcgtacgaac atggcgttcg ctgcaaaaac agcgttcggc cagttcgctg
    15301 acggtgacgg tttgcggctt tccggcggaa ggttgccaca gacgttgata ctggttaaga
    15361 cggttgagca gtcgcatata aacccggaac aatattattt aactattcac tattacttcc
    15421 gtatatatca ggtgatactc aatcaccatt aaccgtgtca cagagtggag aaagaaatgg
    15481 ctcgtctggc agcatttgat atggatggca ctttattgat gcctgaccat catttaggtg
    15541 agaaaaccct ctctactttg gcgcgactgc gtgaacgcga cattaccctc acttttgcca
    15601 ctgggcgtca tgcgctggag atgcagcata ttctcggggc gctatcgctg gatgcatatt
    15661 tgattaccgg caacggaacg cgcgtgcatt ctctggaagg tgaactttta catcgtgatg
    15721 atttacctgc ggatgtcgcg gagctggtgc tgtatcagca gtgggatacc cgagccagca
    15781 tgcatatctt caatgacgac ggttggttta ccgggaaaga gatccctgcg ttgttgcagg
    15841 catttgtcta tagtggtttt cgttatcaga taatcgatgt caaaaaaatg ccactcggca
    15901 gcgtcaccaa gatctgcttc tgtggcgatc acgacgatct tacacgcttg cagatccagc
    15961 tatacgaagc attaggcgag cgtgcacatt tgtgtttttc cgccacggat tgcctcgaag
    16021 tgctgccggt gggctgcaat aaaggcgctg cattgacggt gctgacccaa catttaggtt
    16081 tatcgttgcg cgattgcatg gcctttggtg atgcgatgaa cgatcgcgaa atgttaggca
    16141 acgtcggtag cggatttatt atgggcaatg cgatgccgca attgcgcgcg gagctcccgc
    16201 atttaccggt gattggacat tgccgaaatc aggctgtctc tcactatttg acgcactggc
    16261 tggactatcc acatctacct tattcccccg aataacgaga tcccttccag caccgggcaa
    16321 ttgcccggtt ttttttgcgt tgaatttgtc attttgtgcc gtggtgttta aaccgcacag
    16381 aataaattgt cgcgatttca cctttaaaat agaattaaaa gagaaaaaaa ttctctgtgg
    16441 aagggctatg ttagataaaa ttgaccgtaa gctgctggcc ttactacagc aggattgtac
    16501 cctctctttg caggcactgg ctgaagccgt taatctgaca accaccccgt gctggaagcg
    16561 tctgaaacgg ctggaggacg acggtattct tatcggcaaa gtcgccctgc tggatccgga
    16621 aaaaataggc ctcggcctga ccgcctttgt gctgataaaa acgcaacatc acagcagcga
    16681 atggtattgc cgctttgtca cggtggttac cgaaatgcca gaagtgctgg ggttctggcg
    16741 catggccggt gaatacgatt atctgatgcg cgtccaggtt gccgacatga aacgctacga
    16801 cgagttttat aagcgtttgg taaacagcgt gccggggctg tcggacgtca cttccagctt
    16861 cgcgatggaa cagattaaat acaccacttc tttacccatc gaataaatat ccagaatcag
    16921 gtcaggacac aacgcgtgcg attatttgct caattaagct ggtatttccg tcgggaatgg
    16981 cgtcgctatc tcggggctgt cgccttgctt gtcattatcg cgatgctgca actggttccg
    17041 ccaaaagtgg ttggtattgt tgttgatggc gtgacagaac aacactttac taccgggcag
    17101 atcctgatgt ggatcgccac catggtgctg attgccgttg tggtttatct cctgcgttac
    17161 gtctggcgtg tattgctgtt tggcgcgtct tatcaactgg ctgttgaact gcgtgaagat
    17221 tattaccgtc agctaagccg gcagcatcct gagttttacc tgcgtcatcg cacgggtgac
    17281 ctcatggctc gtgcgaccaa tgacgtcgat cgcgtcgtgt ttgccgccgg agaaggggtg
    17341 ctgacgctgg tggattcact ggtaatgggc tgcgctgtgt tgattatgat gtctacgcaa
    17401 attagctggc agttgacctt atttgccctg ttgccgatgc cagtgatggc gatcatgatt
    17461 aagcgcaacg gcgatgcctt gcatgaacgc tttaagctgg cgcaggcggc gttttccagt
    17521 cttaatgacc gcacccagga aagcctcacc agtatccgca tgatcaaagc ttttggtctg
    17581 gaagatcgcc agtcggcgtt atttgccgag gatgccgaag ataccggcaa aaaaaacatg
    17641 cgggtggcgc gtattgatgc tcgtttcgac ccgaccatct atatcgcgat tggtatggcg
    17701 aacttgctgg cgattggcgg cggtagttgg atggtggtgc agggcagttt aacgctgggc
    17761 cagctcacca gttttatgat gtatttaggt ctgatgattt ggccaatgct ggcgctggca
    17821 tggatgttta acatagtgga acgtggtagt gctgcgtaca gccgcattcg cgcgatgctg
    17881 gcggaagcgc cggtggtgaa tgatggtagc gaacccgtgc cggaaggtcg tggcgaactg
    17941 gatgtaaata ttcgccagtt cacgtatccg cagactgacc atcctgcgct ggaaaacgtc
    18001 aatttcgccc tgaaacccgg tcagatgctg ggtatttgcg ggccgactgg ttccggcaaa
    18061 agtaccctgt tgtcgctcat tcagcgtcat ttcgacgtca gcgaggggga tatccgcttt
    18121 catgatattc ctctgacgaa gttacaactc gatagctggc gtagccgcct ggcggtagtt
    18181 agccagacac cgttcctttt ttccgacacc gtagcgaaca acatcgcgtt gggttgcccg
    18241 aatgccaccc agcaagagat tgagcatgtc gcgcggttag ccagcgtgca tgacgatatt
    18301 ttgcgtttac cgcaaggtta cgatacagaa gtgggcgagc gcggcgtgat gctttccggt
    18361 gggcaaaaac agcgtatctc cattgctcgt gcgttattag tcaacgcgga aatcctcatc
    18421 cttgatgatg cgctttcggc agtagacgga cgcacagagc accagatcct gcataacctg
    18481 cgtcagtggg ggcagggaag aacggtaatc atcagcgccc atcgcctttc tgcactgacg
    18541 gaagccagtg aaattattgt gatgcagcac ggacatatcg ctcagcgtgg caatcatgat
    18601 gagctggcgc aacaaagcgg ctggtatcgc gatatgtatc gctatcaaca actggaggcg
    18661 gcgctcgacg acgctccgga aattcgcgag gaggccatcg atgcgtagtt ttagccaact
    18721 gtggccgact ctcaagcgtc tgttagcgta cggttcgccg tggcgtaaac cgctggggat
    18781 tgcggtcata atgatgtggg ttgcggcagc ggcagaagtc agtgggccgc tgcttatcag
    18841 ctattttatc gacaatatgg tagcgaaaaa taacctgccg ttgaaagtgg ttgcagggct
    18901 ggctgcggcg tatgttgggc tgcaactgtt tgccgccggg ctacattacg cgcagtcgct
    18961 gctgtttaat cgggcggcag taggcgtagt gcaacagttg cgtaccgacg tgatggatgc
    19021 tgcgttacgc cagccattaa gcgagtttga tacccaaccc gtcgggcagg tgatttcccg
    19081 cgtcactaat gacactgaag tgatccgcga cctctacgtt accgtagtgg caactgtcct
    19141 gcgtagcgcc gcactggtgg gcgcgatgct ggtggcgatg ttcagcctcg actggcgaat
    19201 ggcgctggtg gcgataatga ttttcccggt ggtgctggtg gtaatggtga tatatcagcg
    19261 ttacagcacg ccgattgtcc gtcgtgtgcg cgcttatctg gcagatatca acgacggctt
    19321 taacgaaatt atcaatggca tgagcgttat ccagcagttc cgtcagcagg cgcgatttgg
    19381 cgaacgtatg ggtgaggcca gtcgttcaca ctatatggcg aggatgcaaa ccctgcgcct
    19441 cgacggtttt ctgctgcgtc cgctgctgag tctgttttca tcgctcattc tttgtggctt
    19501 gttgatgctg tttggcttct ccgccagcgg caccattgaa gtgggcgtgt tgtatgcgtt
    19561 catcagttat ttggggcgac ttaacgaacc cttaatcgaa ctgaccacgc agcaggcgat
    19621 gctgcaacag gctgttgttg ctggtgagcg cgtttttgag ctgatggacg gaccgcgcca
    19681 gcaatatggc aatgatgatc gcccgttgca gagcggcacc atcgacgtcg ataacgtgtc
    19741 atttgcttat cgcgatgaca atctggtgct aaagaacatt aatctctctg tgccttcgcg
    19801 caactttgtg gcgctggtcg ggcataccgg cagtggcaaa agcaccctcg ccagtttatt
    19861 gatgggctat tacccgctaa cggaaggtga gattcgcctt gatggtcgtc cattaagtac
    19921 gctaagtcac agcgcgctgc gccagggcgt ggcaatggtg cagcaagatc cggtggtgct
    19981 ggcggatacc ttcctcgcca acgtgacgct gggacgtgat atctctgaag aacgcgtctg
    20041 gcaggcgctg gaaatcgtgc agctggcgga gctggcgcgt agcatgagtg atggtattta
    20101 cacgccgctt ggcgagcagg ggaataatct ctcagtcggg caaaagcaac tgctggcact
    20161 ggcgcgcgtg ctggtggaga cgccgcaaat cctgatcctt gatgaggcaa ccgccagcat
    20221 tgactccggg actgaacagg cgattcaaca tgctctggcg gcggtgcgtg aacatactac
    20281 gcttgtggtg attgctcacc gcttatcgac tattgttgat gccaacacca ttctggtgct
    20341 tcatcgtggg caagccgtgg agcagggcac tcaccagcaa ctgctggcgg cccagggacg
    20401 atactggcag atgtatcaac tgcaacttgc gggcgaagag ctggcagcca gcgtgcgtga
    20461 agaggaatca ttgagcgcct gaatagcgca atatttcatc gttggtgcaa aaatgtaacg
    20521 cactgtgcac tgtcatagtg cgttttcatt ttcaatcttc ttaacttcct gctctctttc
    20581 tcgtttttca tttttggcac accgcttgca ataccttctt cgtgtagcag aaccattacc
    20641 gaattctgac cggaggggat ctatgaagct ggtgaccgtg attatcaaac cattcaaact
    20701 tgaagacgtt cgtgaagcgt tatcttccat tggtattcag ggcctgaccg tcaccgaagt
    20761 gaaaggtttc gggcgtcaga aaggacatgc cgagctgtac cggggggcgg aatacagcgt
    20821 caatttcctg ccaaaagtaa aaattgatgt ggcgattgct gatgaccaac tcgatgaagt
    20881 gatcgatatc gtcagtaagg cggcttacac cggaaaaatt ggcgacggca aaatcttcgt
    20941 cgctgaattg caacgcgtca ttcgtattcg taccggcgaa gccgacgaag cggcgctgta
    21001 atctctggca cacagcaaca ggaacgaaaa atgaagatag cgacgataaa aactgggctt
    21061 gcttcactgg cgatgctgcc gggactggta atggctgcac ctgcggtggc cgataaagcc
    21121 gacaatgcgt ttatgatgat ttgtactgcg ctggtgctgt ttatgactat tccggggatt
    21181 gccctgtttt acggtgggtt gattcgcggc aaaaacgtgc tgtcgatgct gacgcaggtg
    21241 acggtgacgt ttgcactggt ctgtattctc tgggtggttt acggttactc gctggcgttt
    21301 ggtgagggaa acaacttctt cggcaacatt aacgggttga tgctgaaaaa catcgaactg
    21361 acggcggtga tgggcagcat ttatcagtat atccacgtag cgtttcaggg atcgtttgcc
    21421 tgcattaccg taggcttgat agttggagca ctggcggaac gaatccgctt ctcagctgtg
    21481 ttgattttcg tggtggtatg gctgacgctc tcctacattc cgattgcgca tatggtctgg
    21541 ggcggcggtt tgctggcttc tcacggtgca ctggatttcg cgggtggcac ggtggtgcac
    21601 attaacgccg caatcgccgg tctggtgggc gcgtatctga taggcaaacg cgtgggcttc
    21661 ggcaaagagg cgtttaaacc gcacaacctg ccgatggtct tcaccgggac tgccattctc
    21721 tatatcggtt ggtttggctt taacgccggg tcagcgggca cggcgaatga aatcgcagca
    21781 ctggcatttg tgaatactgt ggtcgcaacg gcggcggcaa ttcttggctg gatcttcggt
    21841 gaatgggcgc tgcgtggtaa gccttcactg ctgggagcgt gttcaggcgc gattgccggt
    21901 ctggtcggcg tgacgccagc gtgtggctac attggtgttg gcggcgcgtt gattatcggc
    21961 gtggtagctg gtctggcggg cttgtggggc gttaccatgc tcaaacgctt gttgcgggtg
    22021 gatgatccct gcgatgtctt cggtgtgcac ggcgtttgtg gcattgtcgg ctgtatcatg
    22081 accgggattt ttgctgccag ctcgctgggc ggcgtgggct tcgctgaagg tgtgacgatg
    22141 ggccatcagt tgctggtaca gctggaaagc atcgccatta cgatcgtctg gtccggtgtg
    22201 gtggcattta ttggctacaa actggcggat ctgacggttg gtctgcgtgt gccggaagaa
    22261 caagagcgag aagggctgga cgtcaacagc cacggcgaga atgcctataa cgcgtaacaa
    22321 gcactgcaaa aaacagccgg acggttttca cctccggcta tttttttaat tatgattacg
    22381 cattacccct tcctgaacgg tcgaggcaac cagcacgccg tcctgagtat aaaactcacc
    22441 gcgcacaaag ccgcgtgcgc tggacgccga ggtgctctcc acgctataca gcagccattc
    22501 attcaaatta aacgggcgat ggaaccacat ggaatggtcg atggtcgcta tctgaattcc
    22561 cggttcgaga aaaccgatgc cgtgcggctg tagagctacc ggcaggaagt taagatcaga
    22621 agcgtaaccg agcagatact gatgaacgcg cagatcatcc ggcacgctac catttgcgcg
    22681 gatccacacc tgacgatgcg gttctgcgac gtgacctttc aatgggttat gaaattccac
    22741 cggacggact tccagcggac gatcgcagat gaatttatct ttcagcactg gcggcagcag
    22801 gtgcgccagc gattgggcga tttgtgtttc cgaaggcagg ccatcaggcg ctggcgcgga
    22861 cggcattgtt ttttgatgtt cgaaacccgc ttctggtgcc tggaaagagg ccgtcatata
    22921 aaaaatcggt ttgccgtttt gaatagcagc aacccggcgg gcgctgaagc tgttaccgtc
    22981 acgcagcgtt tcgacatcat aaataatcgg cttcttacta tcgcctgggc gaagaaagta
    23041 gctgtgaaac gaatgtacca gccgctcttc agggacggtc tcttttgcag catacaaggc
    23101 ctgacccacg acctggccgc caaacacctg gcgtaaacct aaatcttcac tctggccgcg
    23161 aaagagtcct tcctcaattt tttccagatt taacaatgtc agtaaatttt ttagcgcctg
    23221 actcatataa ctctccagta acaaagctgc cgcagcaagc caaagtgagt tgagtataac
    23281 gcaaatttgc cactggtccg atgggtgcaa tggtctgaat tacgggctaa ttacaggcag
    23341 aaatgcgtga tgtgtgccac acttgttgac gttactattt tgttaaccac tcttccggcg
    23401 aggaaagtta gcccgctggt gcattgataa taaggagaag tgaatgaaac tcgtgcacat
    23461 ggccagtggt ttagcggttg cgattgcgtt ggcggcttgc gcagataaaa gcgcggatat
    23521 tcagacgcca gcaccggctg caaatacgtc tatttcagca acacaacctg ctatccagca
    23581 accgaatgtc tccggtaccg tctggatccg tcagaaagtc gcactgccgc ctgatgctgt
    23641 gctgaccgtg acactttctg acgcgtcgtt agccgatgcg ccgtcaaaag tgctggcgca
    23701 gaaagcggtg cgtaccgaag gtaaacagtc accattcagc tttgttctgc catttaaccc
    23761 ggcagatgtt cagccgaacg cgcgtattct attgagtgcg gcgattaccg tgaatgacaa
    23821 actggtattt attaccgata ccgttcagcc ggtgatcaat cagggcggaa ctaaagccga
    23881 cctgacattg gtgccggtgc agcaaaccgc cgtgccggtt caggccagcg gtggcgcaac
    23941 ggctaccgta ccttcgactt caccaactca ggtgaatccg tcttcggcag ttccagctcc
    24001 tacgcaatat taagcgcagt taaccctctc cgcccggaga gggttagtag ttccagcgat
    24061 aacgctgcaa gtcgatttgc ccgcttcccg ataccatcac accttctgcc agtaatgcct
    24121 gtcgttgacg ctgtaaatcc ggtccggtta gcgaaattgt gccgtggcga ttaaccaccc
    24181 ggtgccaggg caaggtgctg ccttcgggga gacgctttaa cacaccgcct acctggcgcg
    24241 cggcgcgcgg cgatcccgcc agtttcgcca catcaccgta agtggtgaca tagccttcgg
    24301 gaatagcggc gacgatttgc cagacgcgtt ggggaaatga atcttctttt tccatctttt
    24361 cttcctgagg taatttttca gcataatctg gaaaaacgcc cgagtgaagc cgcattgcgc
    24421 aagaaaccag catctggcac gcaatgggtt gcaattagcc ggggcagcag tgataatgcg
    24481 cctgcgcgtt ggttctcaac gctctcaatg ggggctctgt tggttctccc gcaacgctac
    24541 tctgtttacc aggtcaggtc cggaaggaag cagccaaggc agatgacgcg tgtgccggga
    24601 tgtagctggc agggccccca cccatttctg cctccccccg tttcgtcaaa aaatcccaac
    24661 atggctaaac tttaaccaca actgacgtcg caagaattgt ctggctgcgc agtacgcttc
    24721 ggaggtatgt ctgatgaagt atgttgatgg ttttgttgtt gccgttcctg ccgataaaaa
    24781 ggatgcctat cgggaaatgg ccgctaaggc cgcgccatta tttaaagagt tcggtgcgct
    24841 tcgtattgtc gaatgctggg ccagcgatgt accggatggt aaagtgaccg attttcgtat
    24901 ggcggtgaaa gcggaagaga atgaagaggt tgtctttagc tggattgaat acccttcaaa
    24961 agaggtccgc gacgctgcta atcaaaagat gatgtcggac ccacggatga aagagttcgg
    25021 cgagtccatg ccgtttgacg gcaagcgaat gatctatggt ggattcgaat caatcatcga
    25081 cgagtaggta gcgtgacggg ctgtgcggcg tgccgtcagc cctaaaattc acaaatgttg
    25141 ctcggcccag cgtaagaaat cctctttcgg taatgccttg ctgtagagcc agccctgacc
    25201 gtagtgcacg ccatgctggc gtaaccactc ttcttgttta ctggtttcga ttccctccgc
    25261 taccattttc agtttcagtg tttttgccat ttcgatgata tgcggcgtga catttttata
    25321 ttccagcgca tcaacgaaag atttatcgat cttcagaatg tcgacatcca gatcctgtaa
    25381 ataacttaaa cttgaatacc ccgtaccaaa atcatcgaga taaatttcat ggcccgcctc
    25441 ccggtagcga gaaattatcg gggcgctggt tttcggatcg gcaaactcgc gttcagtgag
    25501 ttcaagcgcg atctgtctgg gattaacctg atagtgattg atcatttcac gcagcaattg
    25561 cgggattttt tccgaggtga gcacggtgga ttcaagattg atcgaaatat gctgctgcgg
    25621 atgctgacgc agccagtcgc ccatatcttc gaagacgctt cttatgatca gtagcgtcaa
    25681 cggctcagaa aggcccgttt gctgtgccag cggaataaaa ctatctggtg acaaccaact
    25741 accgtctgtc tgcggccagc gcgccagtgc ctcagcaccg acaattttgc cattggctaa
    25801 ggagacaatc ggctgatagt gcacgcaaat atcacgattt tcgatagcat cctgcagccg
    25861 atgatgcggt gactggatac ggcgcaaaat acgcagcaca aacatcgctg ccagcaggcc
    25921 aatcaccaac ccgagcggta accaaataaa gacttgccga tgccagcctt tctgcaacat
    25981 tttcgttgaa gaccaggtga tgatcgaaat attcatttcc gggaagggca ggatatcgta
    26041 aatgattcca ttattttcga tatgctcacc gggtgttttt tgtagtctgg taataattcc
    26101 ctgagcaatt tcatcgctgc tggttatcac aacgttatgg gcattgccaa taatggcggc
    26161 atcaatttgc catgagctat agggaatgac atcaataaag gaagcggggt cgatcatgac
    26221 gacataatgt gccgttccca tggcaaccat gtaacggata atgcctaaat cgttatgcga
    26281 tgttaaccag acacgataac catctttcga aattttacct ggctcgggga aggtatcggg
    26341 cgggctttca tgctccagag acgaacactg gggaacgttg ttatcgatat aaaccacttc
    26401 ctgaatatag cgataactgt aagatacccg acgcatttcc atgagatggg cttcgctaca
    26461 ggcagcgcct tgccatcttt ccagctcttg cagcgcatct ttcccttgtg tcgccacctt
    26521 attggcccga atagcgacgc gggaggaata cgtattcagc tcttcaataa atgatgtttc
    26581 tacctgctga tgggccagcc agatgcttaa gccgacaggc agcaatactg aaagaatcag
    26641 tactcccgaa ataaggccga ccagatgtcg tgttctcacc agcatatcct tattaacaca
    26701 gtgtagggaa agtatatctg attgtggaaa aaggctggca ggagataagg aggtagagcc
    26761 gggatttcat gaagggatgg gtggaaacga acgttgtcat ttgaccaggc gcagagcttt
    26821 acgcctggta tgtaacccta acttaagatg ttgattcggc gcgagcgagg gtaaaaatat
    26881 cgtaaaaaga tagttcacct ttacggacga tcattttttg cagttgttct ttatgaacat
    26941 ccgcgacacg cggagagttc aggatggctt caactagcgc ggcaggtaca aagcgcgtgt
    27001 tgtaccattc gccgttatag caaacgcgga aatccagcac gtcgatatct tcatagcggt
    27061 agcgtgggta gacatcgaaa aagaagaaac cattaagcag gataaataat acaaccagta
    27121 gccagacaga gcctgaaagc gtttcggact gcaacatcac tgccagggtg gcaaaaaaag
    27181 caacgtacat accgataaac aaccccggat gtttacggat aaaactgata ctaaagcggg
    27241 gtttgttgtc gcgtttttcg cgggtattta gagactcaat tgtttcggtc agcaggcgtt
    27301 gtatttcggt catcgtttgc cttcgtggta tctgcaactt tccagggaac accctatttt
    27361 aacgtgggaa tagtagaggg aaagtaacag atttaccaca attttgcata acagttgcga
    27421 aaaaccgatt acaatttttt aattattctg gctggattac cgcccacgac aacgttgtcc
    27481 gggacatctt ttgtgacaac tgcacctgag gctaccacga cgttatcacc aatggtcaca
    27541 ccagggttaa tgaccgcacg tccgccaatc cagacgttat taccgatggt gacgggtttc
    27601 cccagttcag caccgctatt acgtgctaca gggtcgatgg gatgtgttgc cgtgtagata
    27661 tgaacgcctg gtgccaacat acagttatca ccaatgcgaa tagggcagac atcaagcatc
    27721 acgcaatcga agttggcgaa aaaattattg ccgagaaaaa tgttataacc atagtcacag
    27781 cgaaacgttg gctcaatata agcctctgtc acctgaccga ataaatcagc aagaatttgc
    27841 tggcgtaatg tgtgctcttc cgccagggaa tgattgtatc ggtgaataag ccgacgagcg
    27901 cgcaggcgat cgcgagataa cgcctcatct gccgagtgat acaactcacc agcaatcatc
    27961 ttttcttttt ctgtgctcat tactgacctc cggttgctga aaatgcggca acacagtaag
    28021 acgaaaagtc agtgaagaga acgttcccat tacatctgtg tgatgaagat cacatagggt
    28081 atgccttcat ggcgaaagaa ttataaaact cttaataaac agccggttat agctccgaaa
    28141 gcgagttatt tattagcgaa taaatttcca tactgaggaa gggatcttgt cgtacagttt
    28201 attcatggtc aattcggcga ggcggtgatc tgcggctgag taaaataccg ccagctcatt
    28261 atctgataat tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa ttgtctggca
    28321 acgacgtaaa cgcattaaat aatcggtttt tgttaaaggt ttttcggaca tacttctacc
    28381 catggttgta ataattctaa caagataaac tcgcaggatt ctctttcgtc gcattgacaa
    28441 aacaacggaa tagtcgatta cctgacttcc gccatttctg aagatcctgc atattaatac
    28501 cataactact gaacaacata aaggtgtcat ccagatattc gtcgatctgc tcaatgagct
    28561 tattgtcttc attatactta attttgtaat taagtgcgaa ggtcgcaata tgctcaatca
    28621 gttcattcaa ctggaggttg atcgccgagg ttgggtcgtt aacccagcca tgattgcttt
    28681 cttcaaggtt tgcaagacag tcatgataca gggtttcaca gagaaactta agctgcgcga
    28741 tatcatgtct tttgggtgag tattcatcca taacgcgtcc ccttcttagc ggttgaacta
    28801 acggacacct ttcgggatgg aaaaaactta ctgacctgga cttgccctct cgtttgttag
    28861 cttaactata agccactctt tgcaggtttt catcgcatta ttacgaaaaa ttacaattag
    28921 gaaggaagcg ggacaggact cactttttca tactagcact aacccgcagc aggtaaaagc
    28981 agtttgctcg aactgttcat aacatttcag gattatcgcc agatgaaatt ggtaacacta
    29041 ataccaggat tgctctgaat atgacgtaat aaccgaggaa tgaataaag
//
LOCUS       DAZGAP010000045        28926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGAP010000045 DAZGAP010000000
VERSION     DAZGAP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.045"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..992
                     /gene="ybhD"
                     /locus_tag="ACHLAM_001648"
     CDS             39..992
                     /gene="ybhD"
                     /locus_tag="ACHLAM_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7853197.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1033..2028)
                     /gene="pgl"
                     /locus_tag="ACHLAM_001649"
     CDS             complement(1033..2028)
                     /gene="pgl"
                     /locus_tag="ACHLAM_001649"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO7853198.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2183..3001
                     /gene="ybhA"
                     /locus_tag="ACHLAM_001650"
     CDS             2183..3001
                     /gene="ybhA"
                     /locus_tag="ACHLAM_001650"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO7853199.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3002..4060)
                     /gene="modC"
                     /locus_tag="ACHLAM_001651"
     CDS             complement(3002..4060)
                     /gene="modC"
                     /locus_tag="ACHLAM_001651"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO7853200.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4063..4752)
                     /gene="modB"
                     /locus_tag="ACHLAM_001652"
     CDS             complement(4063..4752)
                     /gene="modB"
                     /locus_tag="ACHLAM_001652"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO7853201.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4752..5525)
                     /gene="modA"
                     /locus_tag="ACHLAM_001653"
     CDS             complement(4752..5525)
                     /gene="modA"
                     /locus_tag="ACHLAM_001653"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7853202.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5691..5840)
                     /gene="acrZ"
                     /locus_tag="ACHLAM_001654"
     CDS             complement(5691..5840)
                     /gene="acrZ"
                     /locus_tag="ACHLAM_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO7853203.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5969..6757
                     /gene="modE"
                     /locus_tag="ACHLAM_001655"
     CDS             5969..6757
                     /gene="modE"
                     /locus_tag="ACHLAM_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO7853204.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARNHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6825..8297
                     /gene="modF"
                     /locus_tag="ACHLAM_001656"
     CDS             6825..8297
                     /gene="modF"
                     /locus_tag="ACHLAM_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO7853205.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            8558..9574
                     /gene="galE"
                     /locus_tag="ACHLAM_001657"
     CDS             8558..9574
                     /gene="galE"
                     /locus_tag="ACHLAM_001657"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO7853206.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9584..10630
                     /gene="galT"
                     /locus_tag="ACHLAM_001658"
     CDS             9584..10630
                     /gene="galT"
                     /locus_tag="ACHLAM_001658"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO7853207.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10634..11782
                     /gene="galK"
                     /locus_tag="ACHLAM_001659"
     CDS             10634..11782
                     /gene="galK"
                     /locus_tag="ACHLAM_001659"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO7853208.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11776..12816
                     /gene="galM"
                     /locus_tag="ACHLAM_001660"
     CDS             11776..12816
                     /gene="galM"
                     /locus_tag="ACHLAM_001660"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO7853209.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            13019..13771
                     /gene="gpmA"
                     /locus_tag="ACHLAM_001661"
     CDS             13019..13771
                     /gene="gpmA"
                     /locus_tag="ACHLAM_001661"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO7853210.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13919..14971)
                     /gene="aroG"
                     /locus_tag="ACHLAM_001662"
     CDS             complement(13919..14971)
                     /gene="aroG"
                     /locus_tag="ACHLAM_001662"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO7853211.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            15287..15667
                     /gene="ybgS"
                     /locus_tag="ACHLAM_001663"
     CDS             15287..15667
                     /gene="ybgS"
                     /locus_tag="ACHLAM_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO7853212.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15781..16722
                     /gene="zitB"
                     /locus_tag="ACHLAM_001664"
     CDS             15781..16722
                     /gene="zitB"
                     /locus_tag="ACHLAM_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO7853213.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16719..17438)
                     /gene="pnuC"
                     /locus_tag="ACHLAM_001665"
     CDS             complement(16719..17438)
                     /gene="pnuC"
                     /locus_tag="ACHLAM_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO7853214.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17476..18519)
                     /gene="nadA"
                     /locus_tag="ACHLAM_001666"
     CDS             complement(17476..18519)
                     /gene="nadA"
                     /locus_tag="ACHLAM_001666"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO7853215.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCSVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPV
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18767..18842)
                     /locus_tag="ACHLAM_001667"
     tRNA            complement(18767..18842)
                     /locus_tag="ACHLAM_001667"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18807..18809),aa:Lys,seq:ttt)
     gene            complement(18876..18951)
                     /locus_tag="ACHLAM_001668"
     tRNA            complement(18876..18951)
                     /locus_tag="ACHLAM_001668"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18916..18918),aa:Lys,seq:ttt)
     gene            complement(19098..19173)
                     /locus_tag="ACHLAM_001669"
     tRNA            complement(19098..19173)
                     /locus_tag="ACHLAM_001669"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19138..19140),aa:Lys,seq:ttt)
     gene            complement(19177..19252)
                     /locus_tag="ACHLAM_001670"
     tRNA            complement(19177..19252)
                     /locus_tag="ACHLAM_001670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19217..19219),aa:Val,seq:tac)
     gene            complement(19304..19379)
                     /locus_tag="ACHLAM_001671"
     tRNA            complement(19304..19379)
                     /locus_tag="ACHLAM_001671"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19344..19346),aa:Lys,seq:ttt)
     gene            complement(19382..19457)
                     /locus_tag="ACHLAM_001672"
     tRNA            complement(19382..19457)
                     /locus_tag="ACHLAM_001672"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19422..19424),aa:Val,seq:tac)
     gene            complement(19593..19668)
                     /locus_tag="ACHLAM_001673"
     tRNA            complement(19593..19668)
                     /locus_tag="ACHLAM_001673"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19633..19635),aa:Lys,seq:ttt)
     gene            complement(19833..20624)
                     /gene="cpoB"
                     /locus_tag="ACHLAM_001674"
     CDS             complement(19833..20624)
                     /gene="cpoB"
                     /locus_tag="ACHLAM_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO7853216.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     EAIVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20634..21155)
                     /gene="pal"
                     /locus_tag="ACHLAM_001675"
     CDS             complement(20634..21155)
                     /gene="pal"
                     /locus_tag="ACHLAM_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO7853217.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21190..22482)
                     /gene="tolB"
                     /locus_tag="ACHLAM_001676"
     CDS             complement(21190..22482)
                     /gene="tolB"
                     /locus_tag="ACHLAM_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO7853218.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22615..23880)
                     /gene="tolA"
                     /locus_tag="ACHLAM_001677"
     CDS             complement(22615..23880)
                     /gene="tolA"
                     /locus_tag="ACHLAM_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO7853219.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23945..24373)
                     /gene="tolR"
                     /locus_tag="ACHLAM_001678"
     CDS             complement(23945..24373)
                     /gene="tolR"
                     /locus_tag="ACHLAM_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO7853220.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24377..25069)
                     /gene="tolQ"
                     /locus_tag="ACHLAM_001679"
     CDS             complement(24377..25069)
                     /gene="tolQ"
                     /locus_tag="ACHLAM_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO7853221.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25066..25470)
                     /gene="ybgC"
                     /locus_tag="ACHLAM_001680"
     CDS             complement(25066..25470)
                     /gene="ybgC"
                     /locus_tag="ACHLAM_001680"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7853222.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25620..25913)
                     /gene="ybgE"
                     /locus_tag="ACHLAM_001681"
     CDS             complement(25620..25913)
                     /gene="ybgE"
                     /locus_tag="ACHLAM_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO7853223.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25913..26026)
                     /gene="cydX"
                     /locus_tag="ACHLAM_001682"
     CDS             complement(25913..26026)
                     /gene="cydX"
                     /locus_tag="ACHLAM_001682"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO7853224.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26041..27180)
                     /gene="cydB"
                     /locus_tag="ACHLAM_001683"
     CDS             complement(26041..27180)
                     /gene="cydB"
                     /locus_tag="ACHLAM_001683"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7853225.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFHLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27196..28764)
                     /gene="cydA"
                     /locus_tag="ACHLAM_001684"
     CDS             complement(27196..28764)
                     /gene="cydA"
                     /locus_tag="ACHLAM_001684"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO7853226.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7195 a         7698 c         7126 g         6907 t
ORIGIN      
        1 gtcacccaaa atcgtcgctt ccattttccg gagtaataat gaagcatgaa ttatcaagta
       61 tgaaggcatt tgtcatactg gcagagtcca gttcatttaa caatgctgct aaattactca
      121 atattacgca acctgcatta acgcgaagaa taaaaaagat ggaggaagat ttacacattc
      181 aactatttga gcgtacaact cgcaaggtta cgttaacaaa agcaggaaaa aggttgctcc
      241 cggaggcgcg ggaattaata aaaaaattcg atgaaacgct ttttaatatt cgtgatatga
      301 atgcttatca tcgtggtatg gtgacgttag cgtgtattcc gaccgcagta ttttattttt
      361 taccgctggc aatcggtaaa tttaatgagc tgtatcccaa tattaaagtg cggattctgg
      421 aacaaggcac gaataattgc atggaatcgg tgctgtgtaa cgaatcagac tttggcataa
      481 acatgaacaa cgtcacaaat tcatccattg attttacccc actggtcaac gagccgttcg
      541 tgctggcctg tcggcgtgac catccattgg ccaaaaagca gcttgtagaa tggcaggagt
      601 tagtgggtta caaaatgatt ggcgtacgct cctccagcgg caaccgacta ttgatagaac
      661 aacagctagc cgacaagccc tggaagctgg actggtttta cgaagtgcgt catctttcga
      721 cgtcattagg gctggtggag gcgggactgg ggatttcggc gcttcctggt ctggcaatgc
      781 cccatgcgcc ctattcttcg attattggta tcccgctggt ggaaccggtt atacgtcgga
      841 cattggggat tattcgccgc aaagatgccg tgctttctcc ggcagctgaa cgcttttttg
      901 ccttgctgat taatttgtgg actgacgata aagacaattt gtggaccaat atcgtcgaac
      961 gccagagaca tgcgttacag gagataggtt aatacaccgg cgcagaagag actgcgccgg
     1021 gtaaatcagc ggttagtgtg cgttaaccac cacccacatt ggtccctgcc cgaccgcata
     1081 gcggcctttt tcatgcagta gcccctgctc gccaacaatt tcgtataccg agatgtggtg
     1141 agatttttgc ccggcggcaa tcagatactt gccgctgtga tcaacattga agccgcgcgg
     1201 ctgggtttca gttggctgga agccttcttt actcaacacg ctgccatctt ccgaaacgct
     1261 gaaaacagta atcagactgg cggtacggtc gcaggcgtat aaatgacgac catccggggt
     1321 gatatgaata tcagccgccc aacgggtgtc ggagaagttt tccggcatca tatccagcgt
     1381 ctggacacat tcgatattgc catgcggatc tttcagttcc cagacatcca ctgagctgtt
     1441 taactcattg acgcaatacg catattgttc gtttggatgg aataccatat gacgcgggcc
     1501 ggccccttca acggtagtca cttccgcagg gtcctgcgcc accagatgac catcctcgct
     1561 gacagtaaac aggcaaatgc gatcctgctt taatgccgga acccacagcg tacggttgtc
     1621 cggtgagata ttggcggaat ggcaaccgtc cagcccctcg accacatcga cgacgcccac
     1681 tggcaggcca tcttccagac gcgttacgct cacgttaccc gcattgtaag aaccgacaaa
     1741 gacaaactgc ccctggtgat cggtggaaat atgcgtcgga ctacccggca gcgcagactc
     1801 tgcggcaaag gtcagcgcgc catcgtccgg ggcgatacga tacgccagga cgcgaaactc
     1861 agggcgaaca ccaacataga gataacgttt gtccgggctg accaccatcg gctgcacctg
     1921 ccccggcacg tcgacaacct gtgtcagcgt cagtgcgcct tcatgattca ggttccagac
     1981 gtgaatttgc tggctctcag ggctggcgat ataaactgtt tgcttcatga atgctccttt
     2041 gcatttagca actgactgta acagctaaaa ttagtcgctt ttggcggtga atgctgaatt
     2101 aaaacgcaga attttgtccg atagtggtat cggtgtacca ttcgcgcaga cgaaaattct
     2161 taaccttaat ctggaatacg ccatgaccac acgcgtgatt gctctcgact tagacggcac
     2221 cttattgacc ccgaaaaaga ccttgcttcc atcatcgata gaagccctgg cccgcgctcg
     2281 cgaagcaggc tatcaattaa tcatcgtcac aggtcgccat cacgtcgcta ttcatccttt
     2341 ttatcaggcg ctggcgctgg atacacctgc tatttgctgt aatggcacct atttgtatga
     2401 ttatcatgca aaaacagtgc tggaagcgga cccaatgccc gttaataaag ccctgcaact
     2461 cattgagatg ctgaatgaac accacattca cggtctgatg tatgtcgatg atgcaatggt
     2521 ctatgagcac ccgaccggac atgtcattcg cacgtctaac tgggcgcaaa ccctgccgcc
     2581 ggaacagcgt ccgactttca cacaagtcgc ttctctggct gaaacggcgc aacaagttaa
     2641 cgccgtatgg aagttcgccc tgacgcacga tgacctgccg caattgcagc attttggtaa
     2701 gcatgtcgaa catgaactgg gactggagtg tgaatggtcc tggcacgatc aggttgatat
     2761 tgcacgcggc ggcaacagca aaggtaaacg tttgacgaaa tgggttgagg cgcaaggctg
     2821 gtcgatggaa aacgtcgtgg ccttcggcga taacttcaat gatattagta tgctggaagc
     2881 cgctggtaca ggcgtggcga tgggcaacgc cgatgacgca gtaaaagcgc gcgccaacat
     2941 tgtgattggt gataacacca ccgacagcat tgcccagttc atttatagcc acctgattta
     3001 atcaggcggt tatcgacaca cttttaattt gcgcatacag ccacaggcca ggtttgatcg
     3061 ccagttcatc cctggcccac gggctgatac gcgcccacag cgttttaccg ccgacttcca
     3121 gctccacttc cacctggccg ttgtcgtcat aactattaac cacttttgcc cgcaatacgt
     3181 tacgaatgct ggtttgctgc ggcggttgta aaaccagcga gacatcggaa gcctgaatgc
     3241 ggatgcgtag cgcagcttgc agcggttcgt ccagcttatt gacccacaaa tgctgatcgc
     3301 ccagcgccag cgcggtcatc gcgtaatgcg gatgatgctc aagcaccgtc actttcagaa
     3361 tgctactttg ttgctctttc ggcagccacg gattcatcac gctactgccc cacacttcct
     3421 ccagcgcgcc aaaggctttc acctgaccgt tttccagtac catcactctg tctgccagat
     3481 ggaggatctc atccagcgaa tggctgacat acaacatcgg aatgttgatt tcccgtgtca
     3541 gccgttgcag ataaggcaac agttcgcgtt tacgcggaat atccagtgac gccagcggtt
     3601 catccagcaa cagcaattcc ggtgctgtca gcaaagcccg accaatcgcc acgcgctgtt
     3661 tttcacctcc ggacaggctg cctggtaaac ggtcaagcaa cggttcaatg cctaaaagcg
     3721 ccaccagctt atcgaactga tcgaccatac ttttcgccat gccgtagcgc agattgccac
     3781 gcactttgta atgcgggaac agccgcgcat cctgaaaaac atagccaacg cgacgctttt
     3841 ccggcgtcag gcagatacct ttttcggcat catttagtac ccgcccattg aggacaatcc
     3901 gccctttttg cgggcgcgtc agtccactga tggcgttaat cagcgaagtt tttccggcac
     3961 cggagacgcc aaagatagca gtgatgccat tggcgggcag cgtttcatta atggtcaggc
     4021 aatggttgcc caacgtctgg gaaaaattca gttccagcat gattagcgcc ccgcccgttc
     4081 acggctgatt ctggccagcc attctgaaat caacagggag atcatcgcca gtgcaataga
     4141 aataatgcac agtctcgccg ctccactttc cccgccgggg gtctggatca gggtatacat
     4201 ggcagaagga atggttcgcg tttcaccagg aatgttcgac acaaaggtga tggttgcacc
     4261 aaactcaccg agagaacgag caaaagccag taccgtacca acaataatgc ccggtaaggt
     4321 cagcggtaac gtgatagtaa agaaaacgcg ccagcgcccg gcacccagtg ttcttgcggc
     4381 ctgttccagt ttgacatcaa ccccttccag cgccagacga attgcccgca ccatcagcgg
     4441 aaacgacatg acggcggcag cgagaaccgc gccgcgccag ctaaaggcga aggtaatgcc
     4501 aaaccagtca tacagacgtt caccgataaa tccgcgccgt cccatcgaaa ctaataataa
     4561 gtaaccgacg accacgggcg gtaacaccag cggtagatgc agtacgctgt cgagcagagc
     4621 tttgcccgga aacgtgcaac gcaccagtaa ccaggcaaaa aagatcccaa acggcaggct
     4681 aaacagcaca gccagggaag aaactttcag gcttagtaaa accgcctgcc attctggatc
     4741 ggtcagtatc attacttggt tgtaaatccg taacgtttaa agatttccgc agcctgcggt
     4801 cccttcagat aatcataaaa tgctttcacc gtcgcattgt tatgcccttc cacaacagca
     4861 accggatatt ccactttttt atgcgaatct tccgggaagg tggcaaccac ttttacccct
     4921 ttgctggcaa ctgcgtcaga accgtaaaca atacccagcg gcgcttcatt gcgttcgacc
     4981 aacgccagcg cgccacgaac atcttccgcc ggagccagtt tcggggagag cgtatcccat
     5041 gcgcccagtt tttgcagtgc ttcttttgca taaatgccag cgggaacatg ttccggatcg
     5101 ccaaccgcca gacgaccgcc attcagcagc gaagtccagt tggttttgct gtcgatggtg
     5161 aaatctttct gctcgctggc tttcggtgct acaacgacca ggctattgcc gagcagtgtc
     5221 tggcgcgaag ccgtatcgat cgctttttta tcaaccgcat aatccatcca tttctgatcg
     5281 gcagaaataa acagatccgc aggcgcaccc gcttcaatct gacgggccaa agtagatgac
     5341 gaagcgaaag aagaaaccac atccacgcct ttctcttttt tatactgcgt agcaatgtcc
     5401 tgcattgcgt tagtcagtga tgcggcggcg aacaccgtga ttttcccttc atctgccagt
     5461 gcattgccag caacagcgaa agagagtgcc gccccggcaa acaagttcaa ccatttacga
     5521 gccattggta accccttaat ctaacgttat gtaggcgaca atataacggc taactcagaa
     5581 ttttcccagc agttattgct aacctttgtg aggtagatga gaaaaaatat cggcaggaaa
     5641 agcaggaggt tgagaaaaag aaatgcccga ctaagcgggc attcagggat tcaatgattt
     5701 tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc accaagaccg
     5761 taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc gaataccaga
     5821 ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat tctaaccgct
     5881 aagcaaagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc
     5941 acaataagac tttttccagg atattgttat gcaggccgaa atccttctca cccttaagct
     6001 ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca ttgcgctttc
     6061 cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg catgggatgc
     6121 cattaacgag atgaatcagt taagtgagca tattctggtt gagcgcgcaa caggcggtaa
     6181 aggtggaggc ggcgcagtac tgacccgcta tggtcagcga ctgattcagc tctatgactt
     6241 actggcgcaa atccagcaaa aagccttcga tgtgttaagt gacgatgacg ccctgccgct
     6301 gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc gtaaccaatg
     6361 gtttggcacc atcaccgccc gcaatcatga tgacgttcaa cagcatgttg atgtcttact
     6421 ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg cgcgtctggg
     6481 gctggatgaa ggcaaagaag tgttgatatt actaaaagcg ccgtgggtag gcattactca
     6541 ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta gccatattga
     6601 gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc
     6661 cacagtgccg gtaaatgaag cgacttctct tcagcaagga cagaatgtca cggcctactt
     6721 taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa
     6781 acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa
     6841 ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct cagctaacgt
     6901 taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagcgggaaa tcggcgctgg
     6961 cccgcgcgca ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc cagttttccc
     7021 acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa tggcagcgga
     7081 ataacaccga tatgctcggc cctggcgaag atgacaccgg gcgtaccacg gctgaaatca
     7141 ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag ttcggtatta
     7201 ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga aaaaccctgc
     7261 tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg ttcgatggcc
     7321 tggatgttgc ctcacgtcag cagctggctg agctactcgc ctcgttacat cagtccggta
     7381 ttactctggt actggtgctc aatcgcttcg atgagatccc ggaatttgtc cagtttgctg
     7441 gcgtgctggc ggattgtacg ttagcggaaa cgggcgctaa agaggaactg ctccagcaag
     7501 cactcgtcgc gcaactggca catagcgaac agcttgaagg tgtgcaactg ccggagccgg
     7561 atgaaccttc agcacgtcac gccttacccg ccaacgaacc gcgcattgtg ctgaacaacg
     7621 gcgtggtttc ttataacgat cgccccattc ttaataacct tagctggcag gtgaatccag
     7681 gcgagcactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta ttaagcctga
     7741 ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcgga cgacgtcgcg
     7801 gcagcggcga aaccatctgg gatatcaaaa agcatatcgg ttacgtcagc agtagtttgc
     7861 atctggatta ccgggtcagc actaccgtgc gtaatgtgat cctttctggc tattttgatt
     7921 cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag cagtggctgg
     7981 atattctcgg cattgataaa cgcacggctg acgctccgtt ccatagtctt tcctggggac
     8041 agcagcgtct ggcgctgatc gtccgcgcgc tggtgaaaca tccgacgttg cttattctcg
     8101 atgaaccact acaggggctt gatccgctca atcgccagct tatccgccgg tttgttgatg
     8161 tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccatgct gaagatgcgc
     8221 ctgcctgtat tacccatcgt cttgagttcg tgccggacgg tgacttttat cgctatgcgc
     8281 tgacaaaaat aaactgagtc ggtaatgctg actgtaccgg aggcggccta agcgccctct
     8341 ccggcctaca gttcgacgca tccaggcatg aaaccgcgac ttttttcaga taaaaagctc
     8401 aatcattcat aaaccctctg ttttataatc cattaatggc gcataaaaaa cggctaaatt
     8461 cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct
     8521 atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg ttaccggtgg
     8581 tagcggttac attggaagtc atacctgtgt gcaattactg caaaacggtc atgatgtcat
     8641 cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg
     8701 cggcaaacat ccaacgtttg ttgaaggcga tatccgtaac gaagcgttga tgaccgagat
     8761 cctgcacgat cacgctatcg acactgtgat ccacttcgcc gggctgaaag ccgttggcga
     8821 atcggtacaa aaaccgcttg aatattacga caacaatgtc aacggtaccc tgcgcctgat
     8881 tagcgccatg cgcgccgcga acgtcaaaaa ctttattttt agctcctccg ccaccgtcta
     8941 tggcgatcag cccaaaattc catacgttga aagcttcccg accggcacac cgcaaagccc
     9001 ttacggcaaa agcaaactga tggtggaaca gatcctcacc gatctgcaaa aagcccagcc
     9061 ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc cgtcgggcga
     9121 tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg cccaggttgc
     9181 tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagacggtac
     9241 tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga
     9301 aaaactggcg aacaagccag gcgtacacat ctataacctc ggcgctggcg taggcagcag
     9361 cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta attatcattt
     9421 tgcaccgcgt cgcgagggcg accttccggc ctactgggcg gacgccagca aagccgaccg
     9481 tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg
     9541 gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc
     9601 cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc tggtttcacc
     9661 gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc
     9721 tgctcatgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa
     9781 ccccgattac accgggactt acgttttcac taatgacttt gcggctttga tgtctgacac
     9841 accagatgcg ccggaaagtc acgatccgct gatgcgttgc cagagcgcgc gtggcaccag
     9901 tcgggtgatc tgcttttcac cggatcacag caaaacgttg ccagaactca gcgttgcggc
     9961 attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gaactgggga aaacgtaccc
    10021 atgggtgcag gtctttgaaa acaaaggcgc ggcgatgggc tgctctaacc cgcatccgca
    10081 cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag accgtctgca
    10141 aaaagaatat ttcgccgaac agaaatcgcc aatgctggtg gattatgttc agcgcgagct
    10201 ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg taccttactg
    10261 ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttac ggatcaccga
    10321 tttgaccgac gcccagcgca gcgatttggc actggcgttg aaaaagctga ccagtcgtta
    10381 tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg cgccatttaa
    10441 tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgttgcgctc
    10501 tgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagaaaccc agcgagacct
    10561 gaccgcagaa caggccgcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc
    10621 cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg
    10681 ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac
    10741 cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagttg
    10801 tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa atcagctcga
    10861 cgagttttcc ctcgatgcga ccattgtcgc gcatgaaaac tatcaatggg ctaactacgt
    10921 tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggcg tggacatggt
    10981 gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac tggaagtcgc
    11041 ggtcggaacc gtattgcagc agctttatca tttgccgctg gacggcgcac aaatcgcgct
    11101 taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca tggatcagct
    11161 aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct cactggggac
    11221 taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc attaacagta acttcaaacg
    11281 taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaactg gtgcgcgttt
    11341 cttccagcag ccagcgctgc gcgatgtcac cattgaagag ttcaacgctg ttgcgcatga
    11401 actggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt
    11461 tgaagctgcc agcgcactgg agcaaggcga cctgaaacgt atgggcgagt tgatggcgga
    11521 gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg acactctggt
    11581 agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt
    11641 tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac agcaagctgt
    11701 cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc
    11761 acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac
    11821 cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga ctggggtgcg
    11881 actttacttt ccgcccgtat tccgttttcc gatggcagcg tccgcgaggc gctgctcggc
    11941 tgtgccagcc cggaatgcta tcaggatcag gccgcatttc tgggggcctc tattggtcgt
    12001 tatgccaacc gtatcgccaa tagccgttat acctttgacg gtgaaaccgt gacgctttca
    12061 ccaagtcagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg
    12121 cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat
    12181 cagggcttcc cgggtaatct cggagcgacg gtacaatatc gtctgaccga cgataaccgt
    12241 atctctatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac
    12301 gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt gcagattctg
    12361 gcggacgaat atctgccggt tgatgaaggc ggcattcccc acgacggcct gaaatctgtc
    12421 gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac
    12481 gacgatcagc gcaaagtgaa aggttacgat cacgcgttct tgttacaggc caaaggcgat
    12541 ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac
    12601 accaccgctc cggctctgca attctactcc ggcaactacc tcggcggcac accgtcgcgg
    12661 ggaaccgaac cttacaccga ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac
    12721 agcccgaacc accctgaatg gccgcaaccg gactgtttcc tgcgtcctgg cgaagagtat
    12781 tccagcctga cggaatatca gtttattgct cagtaatgtt aagccctcgt catgagggct
    12841 ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact caatattcac
    12901 aacaacctta cactgcgaca ctattttcgc tatggttatg cgtaagcatt gctgttgctt
    12961 cgtcacggca atataatgag aattattatc attaaaagat gatttgagga gtaagtatat
    13021 ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg
    13081 tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc
    13141 aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacacct ctgtgctgaa
    13201 acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat ggctgcccgt
    13261 tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc
    13321 agaaaccgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg gttttgcagt
    13381 gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc cacgttacgc
    13441 gaagctgagc gagaaagaac tgccactgac ggaaagcctg gcgctgacca ttgaccgcgt
    13501 gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc gcgtaatcat
    13561 cgctgcacac ggtaactcgt tacgtgcgct ggtgaaatat cttgataaca tgagcgaaga
    13621 agagattctt gagctgaata tcccgactgg tgtgccactg gtgtatgagt tcgacgagaa
    13681 tttcaaaccg ttgaaacgct attatctggg taatgctgac gagatcgcgg cgaaagcagc
    13741 ggccgttgcg aaccagggta aagcgaagta aacgtcattc gcttaaaaat gagaaagccg
    13801 actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca ttgcctgatg
    13861 ctacgctgcg cgtcttatca gggctgcaaa cgtacagtca tggcgattcg actataaatt
    13921 acccgcgacg cgcttttact gcattcgcca gttgacgtaa cagagcatcg gtatcatccc
    13981 agccaatgca ggcatcggtg atgctcttac cataggccag cggttcccca ctctcgaggc
    14041 tctgattgcc ttccaccaga tggctttcca ccatcacgcc aataatagcc ttttcgccac
    14101 cggcaatctg ctggcaaacg tcagcacaaa catccatctg ctttttgaat tgttttgacg
    14161 agttagcatg gctgaaatcg atcatcacct gtgctggcag gcctgctttg ttcagccctt
    14221 ctttcacttc agcaacgtgc ttcgcgctgt agttaggctc tttaccgccg cgcagaatga
    14281 tatggcaatc gccgttaccg ctggtattca caatcgccga atgcccccat ttcgttacgg
    14341 acaggaagca gtgcggcgca ccggcggcat taatggcatc gatagccact ttaatcgtac
    14401 cgtcggtgcc atttttgaag ccgaccggac aagaaagccc tgatgccagt tcgcggtgca
    14461 cctgcgattc ggtggtacgt gcgccaattg cgccccagct catcaggtca gcgagatatt
    14521 gtggggtgat catatccagg aattcacccg ccgctggcag accgctgtcg ttaatatcaa
    14581 gcagcaattt acgggcaata cgcagaccgt cgttgatctg gaagctgtta tccatatgcg
    14641 gatcgttaat cagccctttc cagcccaccg tggtacgcgg cttttcaaaa tagacgcgca
    14701 tcacgatttc cagctcatct tgcagctctt cacgcagcgt cagcaagcga gtggcatact
    14761 ctttcgccgc gacaggatca tgaattgagc acggaccaat caccaccaac aggcgatcat
    14821 cattaccttt caggatctta tggatcgctt ttcgggcatg ggcgaccgta ttcgcggcat
    14881 tttcagtagc ggggaatttt tccagcaatg cgactggagg aagtaactct ttgatttctt
    14941 tgatgcgtaa atcgtcgttc tgataattca tgtctgttcc agtgttgcca tacttatctt
    15001 agtgaatgca atacttccaa tctatatctt ccgtcagaat gtgtaaacgg ggttttacac
    15061 tatgaacgga ttaatcctgg aattagcaag aaaatcgcca gattgtcgcg aaaaacgaga
    15121 tctctcctac aatttctaac tgtaactcct ttgtttgtta attatttcaa gattctctgc
    15181 tgggtatctt tgcctggctg aaatcttaaa ccattgcctt atattcacct gcaaatgcac
    15241 tgttggaaga ggttatccga cataacgacc ataacaggag catcctatga aaatgacaaa
    15301 actggccaca ctatttttga ctgccactct aagccttgcc agcggtgccg cactggctgc
    15361 cgatagcgga gcgcaaagca ataacggcca ggcaaacgcc gcagctgatg cgggccaggt
    15421 agcccctgat gcccgtgaaa atgtcgcgcc aaataacgtc gacaataacg gggtaaatac
    15481 cggttctggc ggcacaatgc tgcatccgga tggttcttca atgaacaatg acggcatgac
    15541 caaagatgaa gagcacaaga acaccatgtg caaagatggt cgctgcccgg atatcaataa
    15601 aaaagtccaa accggtgatg gcatcaacaa tgatgtcgat accaaaaccg acggtaccac
    15661 gcagtaaccc tgcgaaatag ccaataatgg gagagcggag tctctccctt ttaatttttc
    15721 ccttctaaaa gcagtatgat gtcataaagt taagtgagaa taattaaggg atgactcgtc
    15781 atggcgcact cacactcaca cacttcctca cacctgccag aagataataa tgctcgtcgc
    15841 ttgttgtatg ctttcggcgt gactgccggg tttatgctcg tagaagtcat tggtggtttt
    15901 ctttccggtt ctctggcatt gctggccgat gcgggtcata tgttgaccga tactgccgcc
    15961 ctgcttttcg ccctactcgc agtgcaattt tcccgtcgtc ctcccaccat tcgccacact
    16021 ttcggttggc taagattgac cacgctcgcg gcgtttgtga atgccatcgc cctggtggtg
    16081 attactattt tgattgtctg ggaggcgata gaacgcttcc gcactccgcg tccggtcgag
    16141 ggcggcatga tgatggcaat tgccgtggct gggctgctgg caaatatact ttctttctgg
    16201 ttacttcatc acggcagcga agagaaaaac ctcaacgtgc gagcagcggc actgcatgtg
    16261 ctcggggacc tgctgggttc ggttggggct attattgccg cgttgattat tatctggacc
    16321 ggctggacac ctgctgaccc cattctctca atactggtgt cgcttctggt tctgcgtagc
    16381 gcgtggcgac tgttgaaaga tagcgtgaat gaattacttg aaggtgcacc ggtatcgctg
    16441 gatatcgccg aactgaagcg tcgtatgtgc cgggaaatcc cggaagtccg caatgtgcac
    16501 catgtgcatg tatggatggt aggcgagaag ccggtgatga cgctgcatgt gcaggtgatc
    16561 ccgccacacg atcacgatgc cttgttagat cagatccaac actacctgat ggatcactat
    16621 cagattgagc acgccactat tcagatggaa tatcaacctt gtcatgggcc agactgccac
    16681 ctgaatgaag gggtgtctgg ccattcacat catcaccatt aatgggacag cgcgcgtgaa
    16741 cctctttcgc gtgcgctgtt gatccacatc cggctgccgt tgagggcaat aaaggtcagg
    16801 atgatgtact ccagagacat ggcgtaaacg ccctgaagtg caaaaataac gacgctaatc
    16861 acgttaataa tcacccacaa cagccagttt tccacatact tacgcgtcat cagaatcatt
    16921 gccacgatag ataacaccat catgcatgaa tcccagaacg ggaaagcgtc cggctgtagt
    16981 tcaggcatcg caacctgtaa tcctaatgct tgcatgatca tgactgccac gcgggtcaaa
    17041 aatgcaaaca ccggattgat aaataccgtc atcagaccaa tcgaaacaac gcaaaccgcc
    17101 aaccagctta gcgccttcgg caatggcaac cagcgaattt tcaactccgc ctcgttctga
    17161 ctggtttgtc gcgaccacgc ataccagccg taaatattcg cggcaaagaa aaacatctgt
    17221 aatagcaggc tggcatacag ctgaatctga aagaaaataa tgccaaacaa ggtgacgttg
    17281 atcaggccaa agaagtagtt gctgatcttc tccagactgg caaggccaat acacagcaac
    17341 ccggcgatcg tgcctaccgc ttcgatccat gagagatcat aaccgcctgc acctattggt
    17401 atatgtacca ggatattctg cacactaaaa aaatccatct ttcccccaaa gcgtaacgtt
    17461 acgccttatt attcgttatc cacgtagtgt agccgcaaaa tccagcatac gattgagcgg
    17521 caccagcgcc ctctctcgca gcctttcatc aacatgaacc tcgtgattgc ttccttcctg
    17581 ttctaatgcc tctgcgatgg cctgaaggcc attcatggcc atccacggac aatgcgcgca
    17641 gctgcggcag gttgcaccct cacctgcggt tggtgcttcc agtaactctt taactggcac
    17701 cgcctgctgc attttgtaga aaataccccg atcggttgcc acaataagcc tctgatgtgg
    17761 caatgttttc gcagcagcga tcagttgact ggtggaaccg accgcatccg ccatctctac
    17821 aatagcttgc ggtgattctg gatgcaccag tatggcagca tccgggtatt cttcttgcaa
    17881 gcgggttaac gcctgagtct taaactcatc atgcacaatg caggcaccct gccagcataa
    17941 tatgtctgcg cccgtctgtt tttgcacgta acgccccaga tgtttgtcgg gtgcccagat
    18001 gattttttca cccaaactat ccagatgatc aataagttcg acggcaatgc ttgaagttac
    18061 cacccaatct gcgcgcgctt ttaccgcagc agaagtgttg gcgtagacga cgacagtacg
    18121 atcgggatgg gcatcgcaaa atgcgttaaa ttcttcaaca gagcagccga gatccagtga
    18181 acattcagcc tgaagtgtcg gcatcagaat tgttttttcc ggactgagaa ttttggcggt
    18241 ttctcccata aatctcaccc cagcgactaa caacgtagaa gcgggatgct ttgcaccgaa
    18301 gcgcgccatt tccagagaat cagaaataca gccaccggtt tcttctgcca gttgttgaat
    18361 ttcgggatcg gtatagtagt gggcaaccat caccgcatta cgttctttta gcagacgttt
    18421 tatcttctcg cggtaatacg ctttttcatc aatgcttaac ggcgtcggct tcgggggaaa
    18481 aggataaatc gccgtgtctg gatcaaacat cacgctcatc ttaccatctc gttttacagg
    18541 cttaacgtta aaaccgacat gagcgtaata ttcgctgtta tcggtgaaat ttgttttata
    18601 tgctaaacaa gatagtcgaa tggtggggaa aagtcactcg aattacgcct aattccaccg
    18661 acgtagagtt gcttgcaaag taagctctga agatgtcatc aggttacaaa gtgaaattga
    18721 ggatgcctga atagcaaaaa agcgccttta gggcgctttt ttacattggt gggtcgtgca
    18781 ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac
    18841 cccttgcggg attatgctgc tttaatcatt caggatggtg ggtcgtgcag gattcgaacc
    18901 tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc catacgggtg
    18961 ttacctgaaa gatttctact cggaaccaat ctttaaaatt ggtgggtcgt gcaggatgac
    19021 tcgcttcgct cgccctacgg gccgttgctg gcgcaacgtt atccttcacg cttaacatct
    19081 gagtttgatg ttaaaactgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa
    19141 agtcaactgc tctaccaact gagctaacga cccgagtggt gggtgatgac gggatcgaac
    19201 cgccgacccc ctccttgtaa gggaggtgct ctcccagctg agctaatcac ccgatactgc
    19261 gctgaatact acaaactact tccaccgaac ccaccgtaat tagtggtggg tcgtgcagga
    19321 ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccaactgagc taacgacccg
    19381 gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc
    19441 agctgagcta atcacccgat actacgctgg atactacaac taactaggtt agtggtgggt
    19501 cgtgcaggat gactcgactt cgtctcgccc ttcgggccgt tgctgacgca acgttatcct
    19561 tcacgtttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat tcgaacctgc
    19621 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
    19681 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
    19741 gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag
    19801 aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg tttttgcgcc
    19861 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
    19921 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atctgcagcc
    19981 ttcggtgact tcggatagtt tttcactacc gaagcaaaat aatacgccgc atcatctttt
    20041 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
    20101 tcagggtaat ttttgatgaa attctgaaat gccactattg cttcatcctg gcgggattta
    20161 tcctgcacca gcgcaattgc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc
    20221 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt
    20281 tgatcaccgc tggttgattg cgccgctgca ccaccgctgc tgaggctatc gatctgcaac
    20341 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
    20401 ccgcgcaggg aatcaatatc ggattgatta tcagagagtt gttgctggag ttgggttaaa
    20461 agctggctgt gagcatttga aatacgctca agttgagtga cgcggtcttc gaccgagcct
    20521 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
    20581 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
    20641 accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag tactgcaggt
    20701 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
    20761 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
    20821 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
    20881 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
    20941 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
    21001 atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat gccttcgctg
    21061 ccgtcattgc tggcgttctt attggaagaa catgccgcaa ttgccataac aggcagagca
    21121 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
    21181 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
    21241 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
    21301 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
    21361 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
    21421 cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
    21481 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
    21541 aacctgtgga cgaccggcct ggtcagaagt aaatgccagg ttctggctgt ccgggaacca
    21601 ggtcggttcg gtattgttac tgcgaccatc cgtcacctgg cggatctgac cagaagccaa
    21661 atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca gtttgctgcc
    21721 gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc
    21781 gccatttgcc agcgtttgga tgaccagcgc ggaacgaccg ctttcgaagg tcacataagc
    21841 cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg aacggtgaac
    21901 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
    21961 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt
    22021 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
    22081 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
    22141 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatacc
    22201 cagcgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
    22261 agcgcgatct aacggattaa atttaccgct gttacgcaag tcggcagcaa cgatgccgcc
    22321 aatatcttca ggtgccgcac cagggcccgc ccactggaaa ggaacaactc caataggacg
    22381 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
    22441 cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg
    22501 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
    22561 aaccccgtgg caaccggtgc ctgatgttga ccgtcggaac agtcaacatc gcgattacgg
    22621 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
    22681 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
    22741 aggtttgata tccagtaaca taccatcggg agccagtttt atgcgcagcg tacaggtttt
    22801 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
    22861 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggctgg
    22921 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
    22981 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
    23041 tgcttttgca gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
    23101 agctgctgcc ttttcagcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
    23161 tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct cagcttctgc
    23221 tttggctttt tcagcagctt cagttgccgc tttctttctt gcttcagctg cagctgcttc
    23281 tgccgcttcc gctttcttct tcagtgccgc cgcggctgcc tcggcttttt tctgcgcttc
    23341 ggctgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt
    23401 cgcagcttct tccgcagctt tagcatccgc ttcggccttc gctttagcat ctgccgccgc
    23461 tttcgctgcc gcttcttccg cttgcttctg ctttaactcg gcctgttttg cggcttcttc
    23521 agcctgcttt ttctgctcct gagcagctaa ccgctctttc tcaagttgct tcaggcgttc
    23581 ctgttcagcc gcttgtttct cacgcagctc ttccgcagcc tgctgttcct tcatcttgcg
    23641 ctgctcatca gaacgcttcg cgcttgattc ctggctttgc atgcgtttgt actgctcaac
    23701 taccgcacct gaatcaacca tgacagcgtc gatggatgaa ccaccgccgc ctccggctga
    23761 agcttctata ttctcatcga acgaactcca gatcagcgcc gcaaataaga tgacatgcag
    23821 cactgctgaa ataattatcg cccgcttgag cttgtcgttt tgttcggttg cctttgacac
    23881 tctcggtttc caaaaactgt tcgcctgtta cccgctctct ttcaagcaag ggaaacgcag
    23941 atgtttagat aggctgcgtc attaaaccaa ccgatttcac acccgcacta tgtaacaagt
    24001 tcagtgcttt aattatttca tcgtaaggca catcttttgc gccaccgatc agaaagaccg
    24061 ttttcgggtt ggccttgaaa cggctggaca cttccgccac cacctgctct ggtggtaaac
    24121 gctccaggcg atctttctca accaccacgg tgtactgacc aataccagac acttcaacaa
    24181 tcactggcgg attatcgtta ctgctcaccg cctgtgattc agtagcgtct ggcagatcga
    24241 cctccacgct ctgggtgatg atgggcgctg tcgccataaa gatcagcaac agcaccagca
    24301 gtacgtccag caacggtaca atgttgattt cggacttgag atcgcgacga cctcgtccac
    24361 gcgctctggc catggcttac cccttgttgc tctcgctaac ggtaaacgcc tggcggtgca
    24421 gaatcgcggt aaactcttcc ataaagttgt cgtaattcag ttccagtttg tttacgcgct
    24481 ggttgagacg gttataagcc attaccgccg gaattgcggc gaacagacca attgccgtcg
    24541 caatcaacgc ttctgcgata ccgggcgcaa ccatttgcag tgttgcttgt tttaccgccc
    24601 cgagggcgat aaaggcgtgc atgatccccc agaccgtacc aaacagacca atatacgggc
    24661 tgatggagcc aaccgtaccg aggaacggaa tgtgcgtttc cagattttca agttcacggt
    24721 tcatggagat acgcatagca cgcgacgccc cttccactac ggcttccggc gcatggctat
    24781 tggcacgatg caggcgcaca aactctttga acccgctgta aaagatttgt tccgaaccag
    24841 tcagattatc ccgtttcccc tgactctctt gatagaggcg agagagttcg attccagacc
    24901 agaatttatc ttcaaacgct tcggcttcac gcgccgctgc gttaagaata cgggtccgct
    24961 ggataataat ggcccaggat gcgattgaaa aaccaatcaa aatcaacatg ataagtttaa
    25021 ccagaaggct agccttcagg aacaaatcaa ggatattcat gtcagtcact gcttaaactc
    25081 cgcgacaata gacttgggaa gcgcacgagg cttcattttg agtgggtcaa cgcaaacaac
    25141 cagaacctct gcttcattca gcaaagtatt ctcggcgttg acaatacgtt gcgtgaaaac
    25201 caaagaggtg ccacgcattg atgttatttc agtctgtatt tcgagcatat cgtcgagccg
    25261 cgcaggtgcg taatattcca ccgtcatttt acgtaccaca aaggcaacgc gttcagccat
    25321 cagcgcctgt tgactgaagt gatgatgacg cagcatctct gtgcgtgctc tttcataaaa
    25381 agcgacgtaa ctggcgtggt acaccacacc accggcatcg gtatcttcat agtagacgcg
    25441 aaccggccat cgaaacagcg ttgtattcac tttacatccc ggtaatgcaa caaaagttag
    25501 agcttttaaa cgctgctact atacgcgcgc gattgaggtt tgggaatgag ggcaagttaa
    25561 gggagcgtaa aaatttatgg gcctggagcg gcccataatt ttttaaagag aattctgatt
    25621 taaaagaaga agaaaatcag cccgacaatg agaacaatat cggcaagcaa tgggcaaaaa
    25681 atcccttgcc aaagaacctt ctgcggacga aagcccacgc cgtgaatcac accagcacag
    25741 acggcccaca tcagcaataa accatgccag atttccagtt cactggtctt cgcggcgaaa
    25801 cgtgatgggt cccaaaacat acatcctgct aacagaagcg ccatcacgaa ggaaagcgcc
    25861 cgcagggggc gcttgtccat taccgcatac aaggttgcga taatcttact catcagatgt
    25921 cttcttgacc ggctttgcct gattcgacgt gctcaagcgc cagcgcggtg attaccccaa
    25981 acgaacaggc aagaagcgtt cccagaatcc atgcgaaata ccacattttt agctccttac
    26041 ttagtacaga gagtgggtgt tacgttcaat atcttctttg gtgatacgac cgaacatttt
    26101 ccagtaacac caggcggtgt agagcagaat gatcggtacc agaaccaccg caacccaggt
    26161 catgacgtta agcgtcagct ggctggaagt tgcatcccac attgtcagac ttgcgttcat
    26221 catggtgctg gacggcatca caaacgggaa cattgcgata ccggctgtca ggatgatgca
    26281 ggccagcgtc agggaggaga acacaaacgc ccacgccgct ttatccatac gtgcagtcag
    26341 gatggtcagc agcggcagaa ccacacccag tgccggaata gcccacagaa ttggcgtgtt
    26401 gttgaagtta accagccatg cgccagcttc acgaaccact tctttattca gtgggttaga
    26461 ggctgcgtaa tggtccattg tcgatttcac gacataacca tcgataccgt acatcaccca
    26521 tacgccagcc agtgcgaaac agaccagtgt caccagtgcc gccacctgag ccgttgcacg
    26581 ggtacgcagg tgcagttcgc ccacggtacg catttgcaga taggttgcgc cctgagtaat
    26641 gatcatccct acgctcacca cgcctgccag caggccgaac gggttaagca ggtggaagaa
    26701 gttaccggtg tagtacagac gcagatattc atcaacgttg aacggtacgc cctgcaacag
    26761 gttaccgaac gctacaccaa tcaccaacgg cggaacgaag ctaccaatga agatgcccca
    26821 gtcccacatg ttacgccagc gggtttcttc aatcttggag cggtagtcaa aaccgaccgg
    26881 acggaagaac aaagacgcca gcacgaggat catcgccaca tagaagccgg agaacgcagc
    26941 ggcatagacc atcggccagg cagcaaagag tgcgccgccc gcggtgataa gccatacctg
    27001 gttaccgtcc cagtgtggtg caatggagtt aatcataatt cgacgctcgg tgtcgttacg
    27061 accgaggaaa cgagtgagca tgcccacccc catgtcgaaa ccgtcagtga ctgcaaaacc
    27121 aatcagcaga acgccaacca gcagccacca gataaaacgc aatacttcat aatcgatcat
    27181 ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg gaagactgct caaagtgata
    27241 gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaat ttgaacatta agaacaattc
    27301 tgccaccagg aacagggtat acaggccgca aatcagcacc attgagaaga tgagatcgcc
    27361 tgcggtcagt gacgagttcg ccacagctgt cggcagcact tcaccgatag cccacggttg
    27421 gcgaccatat tcagccacga accagcccgc ttctacagca atccacggca gcggaatacc
    27481 gtacagcgcg gcgcgcagca gccatttttt ctcgccaatg cggttgcgga tgacactcca
    27541 gaaagagagc gcgatgattg ccagcagcag gaagccacac gccaccatga tacggaacgc
    27601 gaagtacagc ggcgctacac gcggaatgga gtctttggtt gcctgttgaa tctgcgcttc
    27661 agtcgcatca gccacgtttg gcgtatagcg tttcagcagc agaccgtaac cgaggtcttt
    27721 cttcatgcta ttgaactggt cacgaaccgc ctggtcggta gaaccagaac gcagttgctc
    27781 gagcagagag tacgccttca tcccgttacg aatgcgttct tcatgctgca ccatcagttc
    27841 tttcaggccg ataactggag tatccacgga acgcgttgca atgatgccca gcgcgtaagg
    27901 gatctggatc gcaaatttgt tcgtctcctc ttcctgatca ggaatgccga acagagtaaa
    27961 ggcagcaggc gcaggttgcg tttcccactc ggcttcaata gcagccagtt tggttttctg
    28021 cacgtcgccc atttcgtaac cagattcatc acccagaaca ataacagaca gaacggcagc
    28081 cataccgaag ctggcagcga tagcaaaaga gcgtttagcg aaggcgaagt cacgaccttt
    28141 cagcatatac catgcgctga taccgaggat gaacatcgcg ccagtcacat aaccagacgc
    28201 tacagtgtga acgaatttca cctgagcaac cgggttaagc accagctcgg agaagctcac
    28261 catctccata cgcatagttt caaagttgaa atcggacgcg attgggtttt gcatccagcc
    28321 gttcgcaacc agaatccaca gtgcggacag gtttgaaccg agcgccacca gccaggtgac
    28381 acacatatgc tgaactttac ccagacgatc ccaaccgaag aagaacagac ctacaaaggt
    28441 ggattcgagg aagaaggcca tcagaccttc gattgccagc ggcgcaccga agatatcccc
    28501 tacatagtgg gaatagtaag accagttagt cccgaactgg aactccatgg tcagaccggt
    28561 agccacaccc agagcgaagt tgataccaaa caacttgccc cagaacttgg tcatatcttt
    28621 ataaatctgt ttgccggaaa ggacgtagac cgtttccata atggccagca ggaacgccat
    28681 accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa actgtaagcg
    28741 cgacagttcg actatatcta acatcatgac tccttgctca tcgcatgaag actccgagag
    28801 tgaaccccgt tagaaagggt cacacgcatg ccccaataca aaattatttg ctcgcctcta
    28861 tcgttgccat ttcatcaaaa gaggaaaaga caatgataaa aaagttacaa atgtgttaat
    28921 gaataa
//
LOCUS       DAZGAP010000046        28529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGAP010000046 DAZGAP010000000
VERSION     DAZGAP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.046"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHLAM_001685"
     CDS             complement(<1..593)
                     /locus_tag="ACHLAM_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7853227.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHLAM_001686"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHLAM_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO7853228.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHLAM_001687"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHLAM_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO7853229.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHLAM_001688"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHLAM_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO7853230.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHLAM_001689"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHLAM_001689"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO7853231.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHLAM_001690"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHLAM_001690"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO7853232.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHLAM_001691"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHLAM_001691"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO7853233.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHLAM_001692"
     CDS             complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHLAM_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7853234.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHLAM_001693"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHLAM_001693"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7853235.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /gene="slyX"
                     /locus_tag="ACHLAM_001694"
     CDS             6904..7122
                     /gene="slyX"
                     /locus_tag="ACHLAM_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO7853236.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHLAM_001695"
     CDS             complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHLAM_001695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7853237.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHLAM_001696"
     CDS             complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHLAM_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO7853238.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHLAM_001697"
     CDS             complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHLAM_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO7853239.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVTVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHLAM_001698"
     CDS             complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHLAM_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO7853240.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10553..12466
                     /gene="yheS"
                     /locus_tag="ACHLAM_001699"
     CDS             10553..12466
                     /gene="yheS"
                     /locus_tag="ACHLAM_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7853241.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12466..13488
                     /gene="yheT"
                     /locus_tag="ACHLAM_001700"
     CDS             12466..13488
                     /gene="yheT"
                     /locus_tag="ACHLAM_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7853242.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13482..13700
                     /gene="yheU"
                     /locus_tag="ACHLAM_001701"
     CDS             13482..13700
                     /gene="yheU"
                     /locus_tag="ACHLAM_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO7853243.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13754..14623
                     /gene="prkB"
                     /locus_tag="ACHLAM_001702"
     CDS             13754..14623
                     /gene="prkB"
                     /locus_tag="ACHLAM_001702"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO7853244.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHLAM_001703"
     CDS             complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHLAM_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO7853245.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15384..16016
                     /gene="crp"
                     /locus_tag="ACHLAM_001704"
     CDS             15384..16016
                     /gene="crp"
                     /locus_tag="ACHLAM_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO7853246.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHLAM_001705"
     CDS             16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHLAM_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO7853247.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHLAM_001706"
                     /gene_synonym="dapC"
     CDS             complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHLAM_001706"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO7853248.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHLAM_001707"
     CDS             complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHLAM_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO7853249.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHLAM_001708"
     CDS             complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHLAM_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO7853250.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHLAM_001709"
     CDS             complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHLAM_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO7853251.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHLAM_001710"
                     /gene_synonym="rotA"
     CDS             complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHLAM_001710"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO7853252.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHLAM_001711"
     CDS             21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHLAM_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO7853253.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILIV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23275..25818
                     /gene="nirB"
                     /locus_tag="ACHLAM_001712"
     CDS             23275..25818
                     /gene="nirB"
                     /locus_tag="ACHLAM_001712"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO7853254.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHANLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25815..26141
                     /gene="nirD"
                     /locus_tag="ACHLAM_001713"
     CDS             25815..26141
                     /gene="nirD"
                     /locus_tag="ACHLAM_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO7853255.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26268..27074
                     /gene="nirC"
                     /locus_tag="ACHLAM_001714"
     CDS             26268..27074
                     /gene="nirC"
                     /locus_tag="ACHLAM_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO7853256.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27093..28466
                     /gene="cysG"
                     /locus_tag="ACHLAM_001715"
     CDS             27093..28466
                     /gene="cysG"
                     /locus_tag="ACHLAM_001715"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO7853257.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGQVVGLRDK
                     LNWFSNH"
BASE COUNT         6904 a         7674 c         7214 g         6737 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtca
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatcg attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttaatctc
     2041 ttcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag ccaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataatagca gttcgtcgcc ctcacttagc aggcgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc gatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4681 gacgaagcca gccccagtct tccggcctcc gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagcagcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgataaaaaa agacgctgct taactcatgg ccttctgcaa tcagcgcctg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     5101 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcaaacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcggt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagaatt ttccatgtaa cgacccagcg aagcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat cggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttaccttt
     7201 accgccgcag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccaccgca
     7261 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgca ccgtgaacgt ggccatgagc
     7321 cagttcttct tcagtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7381 accggccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7441 aaccggtacc ggaccctggt cggtttcagc caggaaacgc ataccaacct gcagttcatc
     7501 aacgcccata aatacgtctt taggaacgcg ttgcaccagg ttttcgtcgt actgaccgta
     7561 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcgtgac cttccagcgc
     7621 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7681 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7741 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7801 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7861 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7921 ccgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7981 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgccccc gcaataaaac
     8041 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     8101 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     8161 gagaaatttg tacggtgtcg gcatgcattg ggatgagctc ccgcaacatc cgcatatcca
     8221 ggcggcgaaa atgcagttgc gcccgctgcg cctgatgtgg atgcatgcct agcgtgacca
     8281 gcgtcttgcg ccccagctct aacgcactgg agaatgtctc acgagaaaac tgcgtcaccc
     8341 ctgcctgtaa taactcatgc gcctccacac gtccgcgcgc tcgcgcaaga atatgtaaat
     8401 gcggaaagtg ctgctggcat atttccacca gtttcatggt gtcttccggc tcattacagg
     8461 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8521 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggcg ctaatatccc
     8581 gctccagcac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8641 aacgcccgaa gcccacgaca atgacctgcg gtttatcatc gttgacccac ggtttttcgt
     8701 cttcttcttc cggtccgtta aactggcggg atagccattt atccaccagc ttcatcagca
     8761 acggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8821 gaaacaaccg ttgcgaagaa gcggtagaaa agagcacaaa ggcaaactca ccgccctgac
     8881 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatataat cgcgccagca
     8941 gatacagcac gagaattttc accgtcacca gcacaaccac gcttatcact acccacaaca
     9001 gatgggtata aagcaccccg agattgagcg acatgccgac agagataaag aacaaaccga
     9061 gcagcaagcc tttgaaggga tcgatagctg tttccagttc gtggcgatat tcgctttctg
     9121 ccagcaacac gcccgcaata aacgtaccga gcgccatcga cagccccagc gcatccataa
     9181 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     9241 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     9301 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9361 ccaacaacgg cactaacgcc agcgccggga tcactgccag atcctgaaac agcagaaccg
     9421 aaaaacctag ctgcccggat tcgctgcgat tcatcccttt ctcacgcatc agttgcaacg
     9481 ccattgcggt tgaggacatc gcaaggccaa taccaccgac caccgccgcc tgccaggcga
     9541 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcac
     9601 ctacgccaaa aatcgaacgc cgcaattgcc aaagcttgga gggattcaac tccaggccga
     9661 tgataaacat caggaacacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9721 taataaaacc cagcccccac gggccaattg caatccctgc cagcaaatat cccaacacag
     9781 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacaaca
     9841 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9901 cagccagtca ccgtaggctc tggcgtgact cgccagctcc tgtgcgcttt gccgtctcgc
     9961 ccagtaaata atgatgggac tcaaccaatg catccggcac atgcccgccg ctagttcaaa
    10021 ggggcgcaga acatcgctca tcgggtagcg attcaaggcg tcataacggt aagcactttc
    10081 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
    10141 cccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
    10201 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
    10261 ctcacggggg atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac
    10321 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10381 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10441 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10501 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10561 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10621 catcaacccc gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatcgaccct
    10681 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10741 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10801 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10861 acgtaacgac gggcacgcca tcgccactat tcatggcaag ctggatgcta tcgacgcatg
    10921 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10981 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    11041 gctgatttgc cgttcagact tgctgctgct cgatgaaccg actaaccacc tcgatctcga
    11101 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    11161 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    11221 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    11281 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatctgc aaagttatat
    11341 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgta ttaagatgct
    11401 ggagcgtatg gagctgattg ccccggcgca cgttgacaac ccgtttcgtt ttagcttccg
    11461 cgcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11521 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11581 gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgagcttgc
    11641 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11701 tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa catctggcac gtttagcgcc
    11761 gcaggaactg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11821 agtgacggaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11881 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11941 cgacatgcgt caggcactga ctgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    12001 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    12061 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    12121 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcaaacagcg cccaggcacg
    12181 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccaaccgc tgcgtaaaga
    12241 gatcgcccgt ctggaaaaag agatggaaaa gctgaacgca cagctggcgc aggcggaaga
    12301 gaaactcggc gacagcgaac tgtacgatca aagccgtaaa gcggagttga ccgcctgcct
    12361 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcgt ggctggaagc
    12421 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg
    12481 acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg cggctttagc
    12541 aattgtcatc tacaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa attcaccccg
    12601 cactggcagc gactggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12661 cctgcacagg cgcggcataa accgcgttta gtcgtgtttc acgggctcga aggcagtctc
    12721 aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12781 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12841 ggtgaaaccg aagacgccag ctggttttta cgctggctgc agcgcgaatt tggacatgcg
    12901 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tcgcctgttt gctggcaaaa
    12961 gaaggtaata acctcccgat tgatgcagcg gtaattgtct ccgcgccatt tatgctggaa
    13021 gcctgtagtt atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    13081 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    13141 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctaat caccgccaga
    13201 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    13261 aatcggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13321 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13381 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13441 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13501 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13561 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13621 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13681 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13741 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13801 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13861 agccgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13921 ccgcaaagca cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13981 cggcctgctg gaacaaacct tcattgaata tggtcagagc ggcaaaggga aatctcgcaa
    14041 atatctgcat acctacgatg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    14101 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    14161 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    14221 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    14281 agaagcagtg atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc
    14341 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14401 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14461 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14521 catcaatacg ttagtggtac cgggcggcaa aatgggtctg gcaatggaat taattatgct
    14581 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactttgct attacgccgg
    14641 ataaaatact atccggcttc acaatgggat agttaaatca cgcggcaacc acttcatacg
    14701 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14761 agagatcaac cgcacgtgca accgctgcgt ctttcaggtc gcgaccggta acgataaaat
    14821 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14881 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14941 tgcaaccccc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    15001 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    15061 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    15121 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    15181 ggagacacaa agcgaaagct atgctaaaac aatcaggatg ctacagtaat acattgatgt
    15241 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15301 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15361 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15421 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15481 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15541 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15601 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15661 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15721 ttctgatgcg tctgtctgca cagatggcgc gtcgcctgca agtcacttca gagaaagtgg
    15781 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa
    15841 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15901 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15961 atcagaatct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    16021 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    16081 gatttatcac cccgatatta actatgcact gcgacaaacg ctggtgctat gtttgcccgt
    16141 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    16201 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    16261 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    16321 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16381 gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc gccattttta ccctcagtct
    16441 ggcaggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca ctctctggta
    16501 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16561 aagtctgctg taccgcgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16621 gcacaccgac cctgaaaaag cgctgccgcc gctgcttgtg cgccagcaaa aagcggtcga
    16681 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16741 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16801 tttgcatcag ccggaagagg tgcaaaaact ggtcgagcgt agccatgcgg aagaagttat
    16861 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggccgatg acattcttta
    16921 tcatcgcctg ccgacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16981 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    17041 ccgcgtgctg cgcacccaaa aaccgctcta tgcgcgtgac ttactggccg ataaacagcg
    17101 gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    17161 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcactgca
    17221 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacgcaaa atggctatgg
    17281 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17341 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17401 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17461 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17521 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17581 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17641 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17701 aatgcgggtg aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17761 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17821 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17881 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17941 actggagtat gacgaaccgg ggagttctgg cgatgccaat atcatggatg cgccggagat
    18001 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    18061 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    18121 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    18181 gcgttcacgc cgcatccgac atcttttgcc tgacgcgtct tatttacgcc ccaatcacct
    18241 tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat atccgcatct tccaccacca
    18301 gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattacg ccttcctctg
    18361 caccggcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tccgcgccaa
    18421 ttaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca
    18481 aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18541 tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac
    18601 cgtgagaacc aggatgaaat gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg
    18661 ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtcaca ccgtagtgca
    18721 tgtaagcaaa caaatcgcca gtccgcccca tcccgcactg cacttcatca aacaccaata
    18781 atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18841 tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtatgg tcatccatca
    18901 ccgctttcac tgcgtggaga tcgttaaaag gcacgtggat gatgtctgcc ggttttggcc
    18961 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    19021 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    19081 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    19141 cgcgttcggc aaaagttgcc tcaatcagtt tacggccaag acgcagcgcc ggctcattgg
    19201 tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    19261 caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct
    19321 tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19381 cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19441 ccatctcatg atcaccctgt tacgcataaa taaatatgaa attataatca caaaatatgc
    19501 ataaaaaatc actgaatggc aatcagaaat cagcgatgca gaaaattagc cagcagttga
    19561 tgtccttgtt cgctgagaat actttctgga tggaattgca caccttccag atcccactgg
    19621 cgatggcgaa tccccataat ctcgcgggtt tcgctccagg ccgtcacttc aaagcactcc
    19681 ggtaatgagt caggttccac caccagcgaa tggtagcgtg tcacggtgag tggatttgcc
    19741 agccccttaa atacgcccac accattatgt gtaatcggcg aggttttgcc gtgcatgacc
    19801 tttgcggcgc gcacaacttt gccgccaaat gcctgcgcca ttgcctgatg accgaggcag
    19861 acgccaagaa tcggcaagcg tccggcatag tgacgaataa cgtcaaggga gatcccggct
    19921 tcatctggcg tacaggggcc gggtgagatg acaatttttt gtggtttaag ggcgtcgata
    19981 tccgccaacg tcaacgcatc gttgcgctta accaacacat ccgcccccag ttcacaaaag
    20041 tactggtaga ggttccaggt aaaagaatcg tagttatcaa taagcaggat catggcggct
    20101 ccggtacgaa agaaccgcgc tattttactc agattccccg gcttcgctta ccactttgct
    20161 aaatatcatc tgcagcgcgg tgagatcccc cattgcccca ttctgatttg cctgattcca
    20221 ggcctctttt tcaataccct gccagctcag ttgatatccg gcatgaatcg ccagttgctc
    20281 gaagaagatc cgctgtgcca aaccacttcc cacccggaag ggatgcagca cgttgatttc
    20341 acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa ccagataacc
    20401 ttcttcctcc agatcctgca tcagggcatt gccctctttt tcgatataag caaagtggca
    20461 gaacggcgta tcgccctgat aaatatcgac ttcgcgcagt tgccccgccc agtcgaaaat
    20521 atcctgatac aactggcgat gaagagtacg caaatgcggt aaaccccgaa ccaacgggcc
    20581 aagttcaatg gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt ccagccgctg
    20641 ctgctggcgg atgttcagcc ggttacgcat gatatcaagg cctggataaa gatacggatc
    20701 gcgcccttcg ccgaatttat cgctcatagt gcctcctcag cgcttcaaga cgcgctaaag
    20761 cctctgcggc agtaagagtg actaaaggca tctcgacgcc ttcaagacgg cgactggcct
    20821 ggaaattacg attacgctga agctcccaga gacgggactt ttgcttatcg gtgagtttct
    20881 tcacttgatg cctccctgaa cgtgtccatt tgccacaagt ataagcggca aatccgggtt
    20941 acgccgggag agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt acggcaggac
    21001 tttagcggaa aggataacta ccggttttga cggcacattc tggtacggac caacgtcatg
    21061 agtcggcacc tgggaaatct tatcggcaac gtccatgcct ttcaccactt taccaaatac
    21121 cgcgtaaccg aaatcacgct gaccgtggtc aaggaaggcg ttatcggcaa cgttgataaa
    21181 gaactggctg gtggcgctgt ctttgtcagc ggtacgcgcc atcgcgatgg tgccacgcgt
    21241 gttgcgcagg ccgttatcag cttcattttt gattggcggg tttggttttt tctgctgcat
    21301 ctgctcggtg aaaccgccgc cctgaatcat aaagccagga atgacgcggt gaaaggtcgt
    21361 gttgttataa aaaccactgt tcacataatc gacaaagttt tgcacagaca ctggcgcttt
    21421 ttgtttatcc agctccagtt cgatgttacc agctgaggtt gtcaacaata cgtgcgggtc
    21481 ccctttcgct gccattgctg cgggagaaag agcagaaaga gcgaaaacag cagccatcgc
    21541 cgccagggtc gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa aaagcaagtg
    21601 aagagattct aaatagcctt caagaacaag gccatccctt tacctaattt tacgtatctg
    21661 aaatatatgt aacctgagga taacttttta ttccgtgatc aaaatcacct cttaaaatgc
    21721 aatttagcaa ccgattgcaa taaaacattt aatcagatca caaaatcgcc taaaatcgcc
    21781 cgctcgcggc atagcccgct atgccatttt atttttgaac gcaggccaat catgactaac
    21841 agcaatcgca tcaagctcac atggattagc tttctttcct acgcactgac cggtgcgttg
    21901 gttattgtca ccgggatggt gatgggaaat atcgccgatt atttcaatct gcctgtttcc
    21961 agcatgagta acaccttcac cttcctcaat gccggcattt taatctctat cttcctcaac
    22021 gcctggctga tggaaatcgt cccgttgaaa acgcagttac gttttggctt tctcctgatg
    22081 gtgctggcgg ttgccggttt gatgttcagc cacagcctgg cgctgttctc ggcggcgatg
    22141 ttcattctcg gggtggtcag cggcatcacc atgtcgattg gtacattcct ggtaacacaa
    22201 atgtatgaag gacgtcagcg cggttcacgc ctgttattta ccgactcctt cttcagtatg
    22261 gccgggatga ttttcccaat gatcgccgcg tttctgctgg cacgtagcat tgagtggtac
    22321 tgggtttatg cctgcatcgg gctggtgtat gttgctattt ttattctgac cttcggctgt
    22381 gaattcccgg cactgggcaa acatgcgcca aaaacagatg ctccggtaga gaaagaaaag
    22441 tgggggatcg gcgtactgtt tctctctgtt gcggcgctgt gctacatcct cggtcagtta
    22501 ggttttatct cctgggtgcc ggaatatgcc aaaggcctgg gcatgagcct gaacgacgca
    22561 ggcacgctgg taagtaactt ctggatgtcg tacatggtcg gcatgtgggc gttcagcttt
    22621 attcttcgct tctttgattt gcaacgtatc ctgatcgtac tggctggtct ggctgcgatt
    22681 ctgatgtacg tctttaacac cggaacaccg gcatatatgg cgtggtcaat tctcgccctg
    22741 ggcttcttct ccagcgcgat ctataccacc atcatcaccc tgggttcaca gcagaccaaa
    22801 gtaccgtcgc caaaactggt taactttgtc ctgacctgcg ggaccatcgg tactatgttg
    22861 acctttgtgg ttaccggccc gattgttgaa catagtggtc cgcaggcggc gctgctgacg
    22921 gcaaacggtc tgtacgctgt cgtctttgtg atgtgcttcc tgttaggttt cgtcagccgt
    22981 caccgtcagc ataacaccct gacctctcat taattgttca tgccgggcgg cactatcgtc
    23041 gtccggcctt ttcctctctt actctgctac gtacatctat ttctataaat ccgttcaatt
    23101 tgtctgtttt ttgcacaaac atgaaatgtc agacaattcc gtgacttaag aaaatttata
    23161 caaatcagca atatacccct taaggggtat ataaaggtga atttgattta catcaataag
    23221 cggggttgct gaatcgttaa ggtaggcggt aatagaaaag aaatcgaggc aaaaatgagc
    23281 aaagtcagac tcgcaattat cggtaacggt atggtcggcc atcgctttat cgaagatctt
    23341 cttgataaat ctgatgcggc caactttgat attaccgttt tctgtgaaga accgcgcatc
    23401 gcttatgacc gcgtacacct ctcgtcttac ttctctcacc acaccgccga agagctgtcg
    23461 ctggtgcgcg aaggcttcta cgagaaacac ggcatcaaag ttctggtcgg cgaacgcgct
    23521 atcaccatca accgtcagga gaaggtgatt cactccagcg ccggacgtac cgttttttat
    23581 gacaagctga tcatggcgac cggttcctac ccgtggatcc cgccaattaa aggttctgat
    23641 actcaggact gctttgtcta tcgcaccatt gaagacctca acgccattga atcctgcgct
    23701 cgtcgcagca agcgcggtgc cgttgttggt ggcggcctgt taggtctgga agctgctggc
    23761 gcgctgaaaa acttaggcat tgaaacccac gttatcgaat ttgcccctat gctgatggcg
    23821 gaacagcttg atcagatggg cggcgagcag ctgcgtcgca aaatcgaaag tatgggcgtg
    23881 cgcgttcaca ccagcaaaaa cactcttgag attgtgcagg aaggtgttga agcgcgtaaa
    23941 accatgcgtt ttgccgacgg cagcgaactg gaagtcgact ttatcgtctt ctctaccggt
    24001 atccgtccgc gcgataagct ggcaacccag tgtggtctgg acgttgctcc gcgtgggggt
    24061 atcgtcataa atgattcctg ccagacttcc gatccggata tctacgccat cggtgaatgc
    24121 gcaagctgga acaaccgtgt atttggtctg gtagcaccag gctacaaaat ggcgcaggtc
    24181 gccgttgacc atattcttgg tagcgaaaac gcctttgaag gtgcagacct tagcgccaag
    24241 ctgaaactgc tgggcgtaga cgtaggcggt attggtgatg cgcacggtcg cacacctggc
    24301 gcacgtagct acgtttacct cgacgaaagc aaagagatct acaaacgcct tattgtcagc
    24361 gaagacaaca aaaccctgct cggtgcggta ctggtgggcg ataccagcga ctacggcaac
    24421 ctgctgcaac tggtgctgaa tgctatcgag ctgccggaaa acccggattc tctgattctg
    24481 ccggcgcact cgggtagcgg taagccgtct atcggtgttg ataaactgcc ggacagcgcg
    24541 caaatctgct cctgcttcga cgtcaccaaa ggtgatctga ttgctgccat caacaaaggc
    24601 tgccacaccg ttgcggcgct gaaagctgaa accaaagcgg gtactggctg cggtggctgt
    24661 atcccgctgg tcactcaggt actgaacgcg gaactggcga aacagggcat cgaagttaac
    24721 aacaacctgt gcgaacactt tgcttattcg cgtcaggaac tgttccattt gatccgcgtt
    24781 gaaggcatta aaaccttcga agaactgctg gcgaaacacg gcaaaggcta cggttgtgaa
    24841 gtttgtaaac cgaccgtcgg ttcgctgctg gcctcctgct ggaacgaata cattctgaaa
    24901 ccggaacata ctccgctgca ggattctaac gataacttcc tcgctaacat ccagaaagac
    24961 ggcacctact cggtgatccc gcgttctccg ggcggtgaaa tcaccccgga agggctgatg
    25021 gcagtaggtc gtatcgcgcg tgaatttaat ctctacacca aaatcaccgg ctcccagcgc
    25081 ctggcgatgt ttggcgcaca gaaagacgat ctgccggaga tctggcgtca gctgattgaa
    25141 gccggcttcg aaaccggtca tgcctatgcg aaagcactgc gtatggcgaa aacctgcgtg
    25201 ggtagcacct ggtgccgcta cggcgtgggc gacagcgtcg gcctcggcgt ggaactggaa
    25261 aaccgctaca aaggtatccg gactccgcac aaaatgaagt tcggtgtctc cggctgtacc
    25321 cgtgaatgtt cagaagctca gggtaaagac gtgggtatta tcgccaccga aaaaggctgg
    25381 aacctgtatg tttgcggtaa cggcggcatg aaaccgcgtc atgcgaatct gttggcagcg
    25441 gatatcgatc gcgaaacgct gatcaaatat ctcgaccgct tcatgatgtt ctacatccgt
    25501 accgccgaca aactgacgcg taccgcgccg tggttagaaa acctcgaagg tggcatcgat
    25561 tacctgaaag cggtgatcat tgacgacaaa ctggggctga acgcacatct ggaagaagag
    25621 atggcgcgcc tgcgtgaagc ggtagtgtgt gagtggactg aaacggtcaa taccccgtct
    25681 gcgcagactc gcttcaaaca cttcatcaac agcgataagc gtgacccgaa cgtgcagatg
    25741 gtgccagagc gcgaacagca ccgtccggca acgccgtatg aacgtatccc ggtaactctg
    25801 gtggaggaca acgcatgagc cagtggaaag acatctgcaa aatcgatgac atcctgcctg
    25861 aaaccggcgt ctgcgcgctg ttaggtgacg aacaagtagc aattttccgc ccgtatcaca
    25921 gcgatcaggt gtttgcgatc agcaacatcg atccgttctt cgagtccagc gtgctgtcac
    25981 gcgggctgat tgcggaacac cagggcgagc tgtgggtcgc cagcccgctg aaaaaacagc
    26041 gttttcgctt aagtgatggc ttgtgcatgg aagacgaaca gttttccgtc aaacattacg
    26101 aagcacgagt gaaagacggc gtggtgcagc tgcgcggtta atgttttaac gggaggcgca
    26161 atgcctcccc tttttgcatg gtcctgtaat gaccttcggt atattgcagg acatttttta
    26221 aactttttgt tttatttttt tgtttttatt ttttaaagga taatcaaatg tttacagaca
    26281 ctattaataa gtgtgcggct aacgctgcgc gcattgcacg cctgtcggca aataacccgc
    26341 tcggcttttg ggtcagctcc gccatggcgg gcgcgtatgt gggtcttggg atcatcctga
    26401 ttttcacgct cggtaatttg ctcgatccgt ccatacgtcc tctggtgatg ggcgcgacct
    26461 ttggtatcgc cttaacgctg gtgattatcg ccggttctga actgttcacc ggacacacca
    26521 tgttcctcac ctttggggtg aaagcgggca ccatcagcca cgggcaaatg tgggcaatcc
    26581 tgccgcaaac ctggctgggt aatctggtcg gttccgtctt cgttgccatg ctctatagct
    26641 ggggcggcgg tagcctgctg ccggtagata ccagcatcgt tcactccgtc gcgctggcaa
    26701 aaaccactgc accggcaatg gtactgttct tcaaaggtgc attgtgtaac tggctggttt
    26761 gcctggctat ctggatggcg ctgcgcactg aaggggcggc gaaatttatc gctatctggt
    26821 ggtgtctgct ggcatttatc gcgtccggct acgagcactc catcgctaac atgacgctgt
    26881 tcgccctctc ctggttcggc aaccacagcg aagcctacac gctggcgggt attggtcata
    26941 acctgctgtg ggtgacgctg ggtaatactt tatcaggtgc cgtattcatg ggattgggtt
    27001 attggtatgc tacgccgaaa gcgaatcgtc cggttgcgga caaatttaat caaactgaaa
    27061 cagctgccgg ttaattacta aggggttttt acgtggatca tttgcctata ttttgccaat
    27121 tacgcgatcg cgactgtctg attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt
    27181 tactgttaga cgcaggcgct cgcttaacgg tgaatgcatt agcgtttatt ccacagttca
    27241 ccgcatgggc agatgcaggc atgttaaccc tcgtcgaagg gccatttgat gaaagccttc
    27301 tcgacacctg ctggctggcg attgctgcga cggatgatga cgcgcttaac cagcgcgtca
    27361 gtgaagccgc tgaagctcgt cgcatcttct gtaacgtggt cgatgcgccg aaagccgcca
    27421 gctttattat gccgtcaatt attgaccgct caccgctaat ggtcgcggtc tcctctggcg
    27481 gcacctctcc ggttctggcg cgcctgttgc gcgaaaaact cgaatcactg ctgccgctgc
    27541 atctgggcca ggtagcgaaa tacgccgggc aattacgcgg gcgagtgaaa caacagttcg
    27601 ccacgatggg tgagcgtcgc cgtttctggg agaaattgtt cgttaacgac cgcctggcgc
    27661 agtcgctggc aaacaacgat cagaaagcca ttactgaaac caccgaacag ttaatcaacg
    27721 aaccgctcga ccatcgcggt gaagtggtgc tggttggtgc aggtccgggc gatgccgggc
    27781 tgctgacact gaaaggtctg caacaaattc agcaggcaga tgtggtggtc tacgaccgtc
    27841 tggtttctga cgatattatg aatctggtac gccgcgatgc tgatcgcgtt ttcgtcggca
    27901 aacgcgcggg ataccactgc gtaccgcagg aagagataaa ccagatcctg ctacgggaag
    27961 cgcaaaaagg caaacgcgtg gtgcggctga aaggcggcga tccgtttatt tttggccgtg
    28021 gtggcgaaga gctggaaaca ctgtgcaacg cgggtattcc gttctcggtg gttccgggta
    28081 ttaccgcagc ttctggttgc tctgcctatt cgggtattcc gctcacgcat cgcgattatg
    28141 cccagagcgt acgcttaatt accggacact taaaaaccgg tggcgaactg gactgggaaa
    28201 acctggcggc agaaaaacag acgctggtgt tctatatggg gttgaatcag gccgcgacta
    28261 ttcagcaaaa gctgattgaa cacggtatgc ctggcgaaat gccggtggca attgtcgaaa
    28321 acggaacagc agtcacgcag cgcgtgattg acggtacgct cacacaactg ggcgaactgg
    28381 cgcagcaaat gaacagtcca tcgctaatta ttattggtca ggttgttggc ctgcgcgata
    28441 aattgaactg gttctccaac cattaattta accaaggcca gagaattctg gcctttttcg
    28501 tttacaactc tctttatata ttcagcaga
//
LOCUS       DAZGAP010000047        28526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGAP010000047 DAZGAP010000000
VERSION     DAZGAP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.047"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..9556)
                     /gene="cdiA"
                     /locus_tag="ACHLAM_001716"
     CDS             complement(<1..9556)
                     /gene="cdiA"
                     /locus_tag="ACHLAM_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFO7853258.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTWSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGNVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARE
                     GDIILSSSGKLVLKNSLAGGSTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQSRLVT
                     DKNLVLSSSGQIVQNGGELTAGQNAMLSALHLNQTSGAVNAAENVTLTTTDDTTLKGR
                     SVAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGLKVTATDLTSTGSIK
                     SGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLISDDVLTLSATQINNSGTLS
                     GAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGTVSVKGNSLKTTTTA
                     QTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVL
                     VASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLI
                     TGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALALAGDTLSQGRHGRW
                     LTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANRGSVLATGNLTASATGQLTSTGDIM
                     SQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTLHHRSTDNSGTVTGL
                     SGLTLHSADGLTNSGALLSQNSLVLSAGDVTNSGRIQGQNITLDASSLTSSGAVQSAL
                     DLALTLSGDVIAATGSKITALGDARLSGKVLGNQGLISAKTLEVNGDSLSNSGEISGV
                     NSLNVTLSGNLQQHGKMLTGGALNVNARDISNSGQLQGADNRITASSLANSGRVQGES
                     GLTLTLLNALTNQTSGVLLSQNVSALSAPALTNDGTIQGNGKTTLSAATQAHNSGKIL
                     SGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNGTVMAANQATLTGNSLTNRGLFQAA
                     QLNVNTQTITNSGTLLGNQGLTIKGNNLNNAGGKVFSGGDMLAEMVSLSGAGQLVALG
                     NLTLKLTRGLTAQGVIAANKQLSVSSQGDITNGATLQGNGITLNAAGRLTNNGQLTAG
                     NGTTALSGSGIAMNASGSLQAGGDVSLTSRGDITLDAFTGTTGSLMLTAAGAVINTAL
                     LYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVSGAANAEVINTSGNIETTRGDITIR
                     TGHLLNQREGINETKSYIPVENVAVPDGANSVSVRVGDLGEDGWGYYVKSWSGTAGGG
                     FDAWAVPTEKGATRKFLTGTTRVDVGATGGDARISAGNNLLIDADKLDNTGSHLLASG
                     FVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMIPNDGHLQYGDASADDRVTFTLSGA
                     PEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTSTTSNAGRITNTLAAPEINTPAEKN
                     ISPGMAQLAPDGTETLTVTAPDWTDTITRLTIGSGTDLASGIVEGNYPLPSGNNGYFV
                     PSTDPDSPYLITVNPKLDGLGKVDSSLFTGLYDLLRMHPGQAPRETDPAYTDEKQFPG
                     SSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYMLNQIGGRYINGVGSDTDQMRYLMDN
                     AARAQKALGLKFGVALTADQVAALDQSILWYKAVTIKGQTVMVPEVYLSPKDVTLQNG
                     SIISGQNVHLAGGNVTNSGSTLMAQNNLTIDSADSLGNLESGLINAGGALGLKAMGDI
                     NNISATITGKTVRLESLAGNVNNLTRYSHWQLDAPEDSLALKHTYTGSIASVSAMDSL
                     DIRADKNISVTGAEISAGDRAALIAGNDLSLNAIDRVSSRRHANSESHQRSAGLTTIT
                     AGDSVMLSAGRDVSSQGAGIAAEDNITVRAGRDVNLLAEESVTGSSSYSKKKTVIDET
                     VRQQGTEIASGGDTTITAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLET
                     KKKSGGFLSKKTTHTISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLA
                     AGNHVDVLAVTSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSAS
                     TIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSG
                     LTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGD
                     PNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGS
                     QLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVN
                     AAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQ
                     DTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDI
                     TVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDK
                     FQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNPADLSRDPAHANDSI
                     SPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQ
                     RQEYLAKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPEL
                     AHLLKSTEKDPVVNAIAHAILGGAVAAMQGNNVAAGAAGAATGEMAARAIAGMLYPGV
                     KQSDLSEEQKQTISTLATVSAGLAGGLTGNSSASAAVGAQSGKNAVDNNYLSVSEKTE
                     LEIAKQTLKNSKNPAEREKAQQKYDALLEKDIASDKEVIAACGNGNAGSSACASARLK
                     VIASKEGFEDGPYNSKYSQQYADAYGQIVNLLDITSVDVQNQQQVKDAMVSYFMATLG
                     VDQKTAQGYVETTQGLEIAAASMTPLFGQAVANKITALVDKANKYPSGIGFKINQPEH
                     LAQLDGYSQKKGISGAHNADVFNKAVVDNGVKIISETPTGVRGITQVQYEIPTKDAAG
                     NTTGNYKGN"
     gene            complement(9569..11346)
                     /locus_tag="ACHLAM_001717"
                     /pseudo
     CDS             complement(9569..11346)
                     /locus_tag="ACHLAM_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            11846..12235
                     /locus_tag="ACHLAM_001718"
     CDS             11846..12235
                     /locus_tag="ACHLAM_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853259.1"
                     /translation="MKRYIKWLAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDNSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            12299..13357
                     /locus_tag="ACHLAM_001719"
     CDS             12299..13357
                     /locus_tag="ACHLAM_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO7853260.1"
                     /translation="MYEQDTLSTLDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEQTVLNEYAVSVLLDMGLSGRIITCKEGNYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARASWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFQAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            13398..14120
                     /locus_tag="ACHLAM_001720"
     CDS             13398..14120
                     /locus_tag="ACHLAM_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO7853261.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRYQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALMIESGDDWQCETQSAIAVNETTLPQSMLFLQH
                     YLQNAGAFSLSGERVQWRWSRR"
     gene            14117..14938
                     /locus_tag="ACHLAM_001721"
     CDS             14117..14938
                     /locus_tag="ACHLAM_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO7853262.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSHEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            14913..15170
                     /locus_tag="ACHLAM_001722"
     CDS             14913..15170
                     /locus_tag="ACHLAM_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO7853263.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTNAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            15182..15433
                     /locus_tag="ACHLAM_001723"
     CDS             15182..15433
                     /locus_tag="ACHLAM_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7853264.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVKRLLQEG"
     gene            15438..16019
                     /locus_tag="ACHLAM_001724"
     CDS             15438..16019
                     /locus_tag="ACHLAM_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853265.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLAHNSLHWLSPLMALL
                     LLLRLRQPRRQAGPLQAVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            16016..17374
                     /locus_tag="ACHLAM_001725"
     CDS             16016..17374
                     /locus_tag="ACHLAM_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFO7853266.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCILSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            17361..17714
                     /locus_tag="ACHLAM_001726"
     CDS             17361..17714
                     /locus_tag="ACHLAM_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO7853267.1"
                     /translation="MIRHEIERHQVQLQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            17705..19381
                     /locus_tag="ACHLAM_001727"
     CDS             17705..19381
                     /locus_tag="ACHLAM_001727"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7853268.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTCYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQGLQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            19385..19807
                     /locus_tag="ACHLAM_001728"
     CDS             19385..19807
                     /locus_tag="ACHLAM_001728"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO7853269.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            19804..20409
                     /locus_tag="ACHLAM_001729"
     CDS             19804..20409
                     /locus_tag="ACHLAM_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO7853270.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLSDDERQRFAAQ"
     gene            20378..22696
                     /locus_tag="ACHLAM_001730"
     CDS             20378..22696
                     /locus_tag="ACHLAM_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO7853271.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVVLLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILMIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLGRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALQQEIASYYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            22693..23277
                     /locus_tag="ACHLAM_001731"
     CDS             22693..23277
                     /locus_tag="ACHLAM_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO7853272.1"
                     /translation="MIKSTFWRALALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTGWTLRDNGNKRELRNASGKL
                     VTEIIYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            23279..24448
                     /locus_tag="ACHLAM_001732"
     CDS             23279..24448
                     /locus_tag="ACHLAM_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO7853273.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDIG
                     YINLHGTATQLNDQIESMVVNALFGKRIPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            24445..24909
                     /locus_tag="ACHLAM_001733"
     CDS             24445..24909
                     /locus_tag="ACHLAM_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO7853274.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            24909..25640
                     /locus_tag="ACHLAM_001734"
     CDS             24909..25640
                     /locus_tag="ACHLAM_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO7853275.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            25637..26866
                     /locus_tag="ACHLAM_001735"
     CDS             25637..26866
                     /locus_tag="ACHLAM_001735"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO7853276.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARSATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(27040..27309)
                     /locus_tag="ACHLAM_001736"
                     /pseudo
     CDS             complement(27040..27309)
                     /locus_tag="ACHLAM_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27706..>28526)
                     /locus_tag="ACHLAM_001737"
     CDS             complement(27706..>28526)
                     /locus_tag="ACHLAM_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO7853277.1"
                     /translation="TVAVLVPSLTDKASSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     AEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPFSPAQITIETAFFE
                     ASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQNTDLVITMPDAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDNC
                     DQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
BASE COUNT         6233 a         7900 c         7466 g         6927 t
ORIGIN      
        1 catttccttt ataattacca gtcgtattcc cagcagcatc ttttgtgggg atttcatact
       61 gaacctgagt aatacctctg actcctgttg gtgtttcaga aataattttc acaccattat
      121 caaccacagc tttattgaat acatcagcat tatgtgcacc gctaattcct ttcttctgcg
      181 aatatccgtc aagttgagct aagtgttccg gctgattaat tttaaaacca atccctgaag
      241 gatatttatt cgctttatca accaatgcag taattttatt agccacagcc tgaccaaata
      301 atggtgtcat tgaagccgct gcaatttcta atccctgcgt cgtctcgaca taaccttgtg
      361 ctgttttctg gtcaaccccc agcgttgcca tgaagtaact caccatcgca tctttcacct
      421 gctgttgatt ttgtacatca acactggtga tatcgaggag attgactatc tgaccatacg
      481 catcagcgta ttgctggctg tatttcgagt tataagggcc atcttcgaac ccttctttcg
      541 acgcaataac tttcagtctt gcgctggcac acgctgaact acccgcatta ccattaccgc
      601 aggcagcaat aacctcctta tcactggcaa tatctttttc aagcaatgca tcatatttct
      661 gctgtgcctt ttcacgctct gccggatttt tactgttttt aagcgtctgt tttgctatct
      721 caagctctgt cttttcagac acgctcaggt agttattatc aacagcattc ttcccgctct
      781 gtgcgcccac tgctgctgat gcgctgctgt ttccggtaag accgccagcc agccctgctg
      841 acacggttgc cagcgtactt atcgtctgct tctgctcttc actgaggtct gactgtttca
      901 cgcccggata cagcatgccg gcaatcgctc tggctgccat ctctcccgtt gctgcgcctg
      961 ctgcaccggc agcaacatta ttaccctgca ttgccgctac tgcgccaccc agaatggcgt
     1021 gagcaatggc atttactacc ggatcttttt ctgttgattt cagcaggtgc gccagttccg
     1081 gggccgaagc tccggccaga gcacccgcta aattcccccc agccagtccc tgaagcgcgg
     1141 ctgtcgcggc ctgtataccc cgctgaatct cactgccagt gccatacttt tcctgttcct
     1201 ttttgtattc cggcgtatca cgcagtttcg caagatattc ctgacgttgt ttctctgttg
     1261 cattagcagg aagtgtttcg ccggttgctt ccttcgcggc cttcaacgca ttcagttccc
     1321 cctgcgtccg cgcgatatcc gccacctgac tgcctatgtc actgataagc cccactgtct
     1381 gcagacgcct ctgctccttc tccttgtcaa atatcgggct gatactgtca ttagcgtgcg
     1441 caggatcacg gctgaggtct gccggattct gcttctgatt gtccctgtcc cggatggtga
     1501 tagtgccttc tgccactgcg gcctgagtcg ttccttccgc atgtccgctg tgacctccgg
     1561 cggatatcat gccacccggc atgttaccct gaaatttatc cccgaagctg ccaccaccgc
     1621 tcagactgat cccactgtga ctgactttat aatccgcttc gttgtgaagg tcactgaacc
     1681 ccagcgttcc ggtatccagg tggtttttat ccggtgtggc agtggaggca atcaccgcac
     1741 catccagttg ggtatgtctg cccactgtga tgtcgaagcc accatcaccg gcaaacattc
     1801 cggtttgttc agcaacggag tcaaagcggc tcttcatctt atcccgggag gcagcgatgt
     1861 aacctgagcc ggtcatggag ccaaaggtaa aactgccgcc ggcagccacg ctggtctgtt
     1921 tactgtcgta cttactggtg tcctgctggc tgctaatcag caggtcgtgg cccacatcgg
     1981 cgataatcct gttgccgttg acctgagcac cgttcagtac cgtatcccga ccactgttga
     2041 tggtgacggt tttaccgctg tctgttgtgg tttcagtcca ctcagtaccg ttacctttct
     2101 cgctgccttt tgccgcatta acgctggcaa agacgctgat accggcaccg ttaccaccgg
     2161 caccgatact gacacccacg ccaccgccac tgctgctgtt cctgcccgtt gttttttgtg
     2221 tgtttgcagc gccactcaac agaatatcat tcgcggcatc caggcttgtg ttaccaccgg
     2281 ccttaagctg gcttcctgca atcacaatat ctccgcggtt atcgcccctg tttttgccgg
     2341 ttgcgacaac agacagatta ttcccggcat tcagcgtact gccggatact gtgtcacttt
     2401 cagaatgttg ttgtgatttc gatttctgag tggtgagcga caggctgact cccgtcgcat
     2461 tcgggtcacc ggttgcggtg gccattgccg cagcctgtcc ggcctgcaca ccagacagcg
     2521 ctgtctttgt ggcctgcagg gctttcagac gactgtcact gctctccttc gtttcctgtg
     2581 cactggtgac cgcattattg atggcactgc ccactgtgcc ggaaagggca accgtcagcc
     2641 cgcttttctt ctgctcaaat ttttcgtcca cagtacgacg gtcatgcccc gggtcaacca
     2701 ccacactgtc accggtaatg ctgatatccc ggttcgcaat cacatccgaa ccgctgatat
     2761 gagcctgttt gcccgcggta atactgacat taccggcagt ggagccgatg gtactggcac
     2821 tctgactctg cgttgtcccg gcctcgcggc ggtcgtgcgt tgtcttactg ctgccgatgg
     2881 tgaagccgat accgccagta cccatcagac ccgatttctt cgtttcctta aagcgccagg
     2941 acgtatctgt actggtgaca gcaagaacat caacatggtt acccgccgcc agtgacacat
     3001 cccggtcagc caccacatcc gaaccctcta ccgtcaggtt atcaccggcg ttaacggtca
     3061 cgcggttccc cgacagcagg gagcctgctt cacgggtggc actgtcctca ctgatggtgt
     3121 gggtggtttt cttactgaga aaacctccgc tttttttctt cgtttccaga tagtgatagt
     3181 cactttctgt cgccgtggtc agggcaacat cacgaccggc attcacgctg atattgccgg
     3241 ttgcggtaac ggatgacgca acagcggtga tatcccgtcc tgcagtaatg gtggtgtcac
     3301 caccgctggc gatttccgtt ccctgctggc gaacggtttc atcaatgacc gttttctttt
     3361 tcgagtatga actgctgccg gtcacggact cttccgccag cagattaacg tcgcgcccgg
     3421 cccggacagt gatgttgtct tccgctgcga tacctgcacc ctgactgctc acatcccggc
     3481 ctgcactcag catcacactg tcgccggcag taatggtcgt aagcccggcg ctgcgctggt
     3541 gagactcact gtttgcatga cgtctgctgc ttaccctgtc aatggcattc agactgagat
     3601 cattgccggc aataagtgct gcgcggtctc cggcactgat ttctgcccct gtgaccgaaa
     3661 tgtttttatc tgcccggatg tccagactat ccatggcact gacagaggca atgcttcccg
     3721 tataagtgtg tttcagggcg aggctgtctt ccggtgcatc aagctgccag tgtgagtacc
     3781 gggtgagatt attgacattg ccggcgagac tctccagccg gacagtctta ccggtaatcg
     3841 tggcactgat attgttgata tcgcccatcg ctttcagtcc gagagcgcca ccggcattta
     3901 taagcccgga ctcaaggtta cccagactgt cagcgctgtc gatggtgaga ttattctgcg
     3961 ccatcagggt actgccactg ttagtgacat tccctcctgc caggtgcaca ttctgaccgc
     4021 tgataatact gccgttctgc agcgtcacat ctttcggtga cagatacacc tccggcacca
     4081 tcaccgtctg accctttatc gtgacggctt tataccacag aatactttgg tcgagagccg
     4141 ccacctggtc tgctgtcagt gccacaccaa acttcagacc gagcgccttc tgtgcacggg
     4201 cagcgttatc catcaggtag cgcatctggt cggtgtctga accaaccccg ttgatatagc
     4261 gccccccgat ctggttcagc atatagttcg atacatagcg ggtatcaaaa gcggcatccc
     4321 ccaggaagcg ataatctttt tccggtttca gcccgagccg gtcaagaaaa tacgatgagc
     4381 ccgggaattg tttttcatca gtatacgccg ggtctgtttc acgtggtgcc tgcccggggt
     4441 gcatcctgag caggtcatac agtccggtaa acagactgct gtccaccttt ccgagaccat
     4501 ccagtttcgg attcaccgta atcagatatg gactgtcagg gtccgtggac gggacaaaat
     4561 aaccattgtt accggaaggc aggggatagt tcccctccac gatgccggaa gccaggtccg
     4621 tgccactgcc gatcgtcaga cgggtaatgg tgtcagtcca gtccggggct gtgacggtca
     4681 gcgtctctgt gccatccggc gccagttgag ccattcccgg gctgatattt ttctctgccg
     4741 gcgtgttaat ttcaggtgcc gcaagggtat ttgtgatccg tcctgcattt gaggtggtgg
     4801 atgtattact gatatcactg gaaaaaacgg cagtgacatt tttgcctgcc tgaatcacgg
     4861 ctctcagagc ctgaccggtg tctctggtga catattccgg agctccggag agagtaaagg
     4921 taacgcgatc atcggcagaa gcatctccat actgcagatg cccgtcgttg gggatcatgg
     4981 ccagtttgcc gtaatagcga tacacattgt attcatcctg tgtatagcca aagaaggact
     5041 gattattcag ttgtgaaccc gacaggctga caaacccgct ggcaagaaga tgacttcccg
     5101 tattatccag tttgtccgcg tcaatcagca gattattccc ggccgaaatt ctggcatccc
     5161 cccctgttgc accaacatcg acgcgtgttg tgccagtcag aaatttccgg gtggctccct
     5221 tttcagtcgg aacggcccat gcgtcaaaac caccaccggc agtaccgctc caggacttca
     5281 cataatatcc ccatccatct tcacctaaat cgcccactct gactgaaaca gaatttgcac
     5341 catcaggtac tgcgacattc tcaaccggaa tgtatgattt tgtctcattt atgccctccc
     5401 gctgattcag taaatgtccg gttctgatgg tgatatcacc gcgggtggtt tcgatattcc
     5461 cggaggtatt aatcacttca gcattcgcgg caccactgac atctttctgc atcaccaggc
     5521 tgtcacccgc cagcatatcg ccgtgatggt tccggatggt gctggcaaac agcgagaggt
     5581 tattgcccgc atacaacagt gcggtgttaa tgactgcccc ggcggccgtt aacatcagac
     5641 tacccgtcgt gccggtaaaa gcgtcgagag tgatatctcc cctgctggtc aggctgacgt
     5701 ccccgcctgc ctgcaggctg ccgctggcat tcatcgcaat cccgctgccg ctcagtgccg
     5761 tggtgccgtt gccggcggtc agctggccat tgttggtcag tctgcctgca gcattcagcg
     5821 tgataccgtt gccctgcaat gtggcgccat tggtaatatc cccctggctg ctgacggaga
     5881 gttgtttatt tgcggcgata acgccctgtg cggtcaaacc acgggtgagc ttcagcgtca
     5941 ggttaccgag tgcaaccagc tggcccgcgc cgctgagcga aaccatttca gcaagcatat
     6001 caccgccgct gaacaccttc ccgccggcgt tgttcaggtt gttccctttt attgtcaggc
     6061 cctgattacc cagcagcgtg ccgctgttgg tgatggtctg cgtgttgacg ttcagttgtg
     6121 cggcctggaa cagaccacgg ttggtcagac tgttacctgt gagcgttgcc tgattcgccg
     6181 ccatcacagt gccattaccg gcaagcttcg ccacgtttag cagcaggtca gtggcctgta
     6241 gccagccact gccactgtag tccggcgtgg tgaacgtcag ctcaccgccg gaaagaatct
     6301 tgccactgtt gtgggcctgc gttgccgcgc tgagcgtggt tttaccgttg ccctgaatgg
     6361 tgccgtcatt ggtcaacgcg ggtgcggaaa gcgctgatac gttctggctg agaagcactc
     6421 cgctggtctg gttggtcagg gcattcagca atgtaagagt cagcccgctc tcgccctgaa
     6481 cacgacctga gttggccagc gaactggcgg tgattcggtt atccgccccc tgaagctgcc
     6541 cactattgct gatgtctctg gcgttgacgt ttaatgcacc gcccgtcagc attttaccgt
     6601 gctgttgcag attaccactg agtgtcacgt tcaggctgtt aaccccactg atttcgccac
     6661 tgttgctgag cgaatcgcca ttcacttcca gcgtcttcgc gctgattaat ccctggttac
     6721 ccagcacttt gccgctcaga cgggcatcgc ccagggcggt aattttgctg ccggtggcgg
     6781 caataacatc gccactcagg gtcagagcca aatccagcgc actctgaaca gcaccgctgc
     6841 tggtcaggct gctggcatcc agagtgatgt tctgcccctg aatccggccg ctgttggtca
     6901 catctcctgc gctcagcacc agcgagttct ggctcagcag cgcgccactg ttggtaagcc
     6961 cgtccgcgct gtgcagggtc agaccgctca gccccgtgac cgtaccgctg ttgtcagtgc
     7021 ttctgtggtg aagcgtgaca tggttaccct ggacagtgcc gctgttatcc agtgagtttc
     7081 catcaaggga gagcgtgccg gccgaaagca gactgccccg gttgtccgtg gtggctgcgt
     7141 tcagcgtggt gtcaccctgg ctcatgatat cgccggtact ggtcaactga ccggttgccg
     7201 aagcagtaag gttaccggtt gccagcacgg aaccacggtt cgcccatctg tccgcctgca
     7261 cggtgagatt ctgtccctgc gtggtccctg cggtattcag tgttttaccc cggagggaga
     7321 ggtcgcccgc cgtcagccag cgtccgtgac gaccctgtga gagggtgtcg cctgcaagcg
     7381 ccagtgcagc ggcgccctgc aacaggccat cgccatccag cgtggtcgcc ctgacgttca
     7441 gcgtgtcagc ggtgatttta cccggattgc tgagggaggc agtgtttaac gttaaaccat
     7501 tatcaccggt gataagcccg ctgttgcgga tgtccggtat atccagcgtc aggtctgcag
     7561 cactgtacag cgtgccgttc tgctgattat caagcctctg tgtgttaacg gtgagtgagg
     7621 cctccccctg caacagaccg ctgttggtca gggtctgtga ctgtgtattc agggcggaag
     7681 caacaagtac gccgctgctg gtcagttccg gcgcactgag gctgagcgac ggggcgctgc
     7741 ttttcccgct gtgggtgagc ttttcactgg cgttcagggt gaggatatct ctggcagcct
     7801 gtgttccgtc aagctgtgcg ttctgcacat ccacgctgac actgttgccc tgcgtctgcg
     7861 cagtggtcgt ggtcttcaga ctgttgcctt ttacagacac cgtgccaccc gcactggttt
     7921 cacccgccag tgtggaagac gcgctgttca gcatgaggtt accgtcactg tttgtgaccg
     7981 atttttctgt ggtggtcagt gtgtctgcag aagccacaag ttcctttgcc ccggagaggg
     8041 taccgctgtt gtttatctgc gtggcactga gcgtcagcac gtcatcgctg atgagtctgc
     8101 cgcggttttc gagtgtaccg ctgacggtaa cgcgggcact gtcttttgca ccggcatcac
     8161 cggacagtgt ggcattgcgg gcgctgatat cgagtgtgct gccactttta atactgccgg
     8221 tgctggtcag gtcagtggct gtgactttca ggccgttccc ctggacggca ccggtattac
     8281 tgaatgtccc ggttttcacc gtggcatcgc gcccggcaac cagtgtcccg ccgttgttca
     8341 ggctgccgga gctgacggtg agtgttttcc cggcaacgct acggcctttc agtgtggtat
     8401 catcggtggt ggtaagggtg acattttcag ctgcattcac ggccccggaa gtctggttca
     8461 ggtgcagtgc actgagcatg gcgttctgtc cggcagtcag ttcaccaccg ttctgtacaa
     8521 tctgcccgga tgaagacagc accagatttt tatccgtcac cagacgggac tgattcagtg
     8581 tcagtcccgt tgtcccggta acgctgagat ttcctccggc tttgttatcc cctgaaagtg
     8641 agacatccgt tccggttacg gtggtactgc cgccggcaag gctgttcttc aggaccagtt
     8701 ttccggaact gctcagtatg atatcgccct cgcgggcata aaggttacca agattaaccc
     8761 cgacaccact ttcagtggag gtcagatgaa tacgcctggc gtacattcca ccgagagcgc
     8821 cggtatcaac ggcaactttc ggcacattac cttcgccctt cagggcactg acgcgaccat
     8881 ctgcagttat ccggttagcg cctgcagtga cagttaaatc cttcgcatga agcgcggcat
     8941 tcacttccgt tgcacgggca ataatggata cggcatcgct ccaggtgccg tccaggcccg
     9001 cgccattgat ggtgatactg ccttcagtca cctccagcgc ctgcaggctg ccgtcggcat
     9061 tcatcacagg tttgcctgtg gtgagcgtcg cgtgcggcgt gttgataaaa ccacagccgt
     9121 cacaggtgat accatacggg ttggcaacca tcacattcgc cgctttgccg gccacttccg
     9181 tatagccctg cagcaatgaa cggttaccgc cagtcacttc gttgatgata cccttcgctt
     9241 ccccgcccgc tttcaggttc gggttattct gtatcagtcc accaagctgc gtcggattaa
     9301 gcttaccggt ggcattattg agaatcagcc cttccttccc gacgttgtaa tccgtaaacc
     9361 ggttatgcga aatcccggcc ccgttcggcg tggcaatgtt cacgaccggc acaccatttg
     9421 ccgctttatc cattccggcc ccgttttgtg gggtgatgac ggcccccaca gccggtaaca
     9481 acggctgccc ggcgataatc gtactgataa ggtaactcag caggcggtaa gtgaagcgaa
     9541 cgggaggctg atgcatggta ataatccctt aaaacgcgac ggcaacgcgc cagtaaaccg
     9601 tgagatggtc aggggcaagc cagtccgggt aaaccagagg cagtccggca gtgaacgaac
     9661 cggaaacatg gccactggtg gtgctgagcc cggcagcagc accccacagc gtgccggacg
     9721 agtacaggtc atctctgtca gagtgcagcc agccgccgtc cagtgcagtc actgcacgga
     9781 ctgtccccac atatggcagg gagaacagag accaggacag ctcatttcgc agataaccgc
     9841 cgttattacc ggagatatac tgctccttaa agccacgcac tgaactctca cccccgaggc
     9901 tcagttgttc cacaccatga agacggtccg gtgaccactg ggcataagcg ctggtcagcc
     9961 accacaccct gtccgtgacg gggcgctgaa aactggcgct taatgaccat ttccggaact
    10021 gatttacggg caggtctccc cttttcccgt ggtcgctttc tgcgccgaac cagggcatcc
    10081 cccgtgtgaa taccggattc agtgttccga caccccccag aaacttgtgt gtgtgattca
    10141 gcccgacaga aaatgaagtg agtttacggc tgctgccctg aagcagaaca tcatccagat
    10201 aattgtgaat aatgcggtgc tgcagacctc cggtcagtgc tgtcttcatg tccccgttac
    10261 ggaacaggac atgcgacagt cccagccggt gagtctgcag gtctcccgtg gaacgccacc
    10321 gccagccccg gttatcaatg gtgctgaggt agtcactcca tgaatacgtg taatccacca
    10381 gggtatagcc atacggcaga ctgacaccgg cggcaaaatt cctcgcatca tgtgacaccg
    10441 aaaagtcact gctccgtccc ccgctgacaa accagttgtc agccagcccc agcggattat
    10501 taaaggaaag gacaccattt aactgccccg taccggtatt cttctgtccg ctgttgtcga
    10561 tgcccacgct ccctgtgaca ggccattccg gcgatgccgt cagtgtcacc accgaccatc
    10621 cctcacggtc accgggcgat atttcaatct gtaccggctc cgtacgcaga cggttaatct
    10681 gctccatccc ctgctcaata tcccgcaggt tcagaacctt cccctccatt cccgggaaaa
    10741 ccatcttcag ggtgcggcca ggaaggtcag cgccttccgc ccggatttgc tgcagcctgc
    10801 cttccatgac cgttatgtgc agtacgcccc tgaaaggtcc tgctctgtca gaaaggcccg
    10861 gctggtgata tatccccggc gtatatagcc gtccgtcacg gcatccgtga ccgcggtcag
    10921 ccccgtgata ttcagacact gattcaccca cggtgccacc agtctgtcgg tttctgcaga
    10981 cgtcagtcgg gtggccccac tgacaacaat gcgtgacacc gtaaagcagg gaccttcagt
    11041 accggcagac atttccggag acggtatgac agtcagcggc gcactgcgct ccagcgcatc
    11101 acgctgacgc tggttttcat ccagcaactg ctgctgttgc tgctgaattg cactgcggtc
    11161 acccggagac agcatggctg ccggagacga aaatgcgggg ataaaaaaac cagaaaaaac
    11221 ggagagggag agataacata tccccgaaga ggcgcgggcg cgccggaata tatcataaca
    11281 ggggacagac acagcccgga ataaaagaga cgttttcgtc agaatgttgt tctgccggtg
    11341 ctgcatgttc tccccgtatt ctggcctgtg taatgtcagt gtaaattcat gtcaaaaatg
    11401 tgtcagcggg aaatttacac ctgaaaaagt ctgtcatgaa acagctttat gacaagatat
    11461 caaaaaaaat gtcaaaaacg tgatctttct tcagcttttc agaatattga gagcctagag
    11521 gtcaatataa attctgggac acatatgcat ttaccattaa aaccggcaca acaggctgtt
    11581 cagtctcaat ccatgctttc tgttttatcg atctcagaat atcggcagac aaataaaaaa
    11641 ggaatgtcga gagagctaac tctagatcac tctccacctc acaagccatt tacaaaaatg
    11701 atacaggcat atctttggtc cctgccacaa ccagacattg acaatccaat ataaccccaa
    11761 atatatttcg gatacgaagc ttgggattta tacagacatt tactatttaa ttataaaatt
    11821 tgtattacat acataagagg cactaatgaa acgttatata aaatggcttg ccatcacaat
    11881 ttttatcagt atgttgagtg cctgtgcccg tacggctcca gtgcaacaga taagcaccac
    11941 tgtcagtgtg ggtcatactc aggagcaggt taaaaatgcc attttgaaag caggtgcaca
    12001 gcgcaagtgg attatgacgc aagtgtcccc tggagttatt aaagctcgct atcaaacacg
    12061 aaatcacgtt gctgaggttc gcattacata tacagctact tactataaca tcaaatatga
    12121 caatagcctg aatctgcagg cttctgatgg aaaaattcat aaaaactata accgttgggt
    12181 gcgtaacctg gataaagata tacaggttaa cttgtctaca ggagcaacgt tatagtgatg
    12241 ctgatatcgg gctggaatag cccgatatca ttaaacttct ctataagaat ggttatatat
    12301 gtacgaacag gacacactca gcacactgga tgccatcacg gaggcacaac ggattgcttt
    12361 tgctcctatg cttttccagg cagctttgtg cctgcgcaat gcaggaatat tagattattt
    12421 ggatcaacag ggtaaacagg gagctcccct taacgcgatt actgaacaga cagtcctgaa
    12481 tgaatatgcc gtgagtgtgt tactggatat ggggttaagc gggcgaatta ttacctgtaa
    12541 agagggaaat tattacctgg cgaaaatcgg tcattacctc ctgcatgaca ctatgactcg
    12601 cgtgaatatg gatttcaccc aggatgtctg ttaccagggg cttttcttcc ttgccgattc
    12661 actcaatgaa ggaaaacctt ccgggttaaa agtatttggc gactggccga cgatatatcc
    12721 ggcactttcg caattaccag atgctgctcg tgctagctgg tttgcatttg atcattacta
    12781 ttcagatggc gcatttaacg ccgcactgcc ttatgtattc gccaacaacc ccacaacctt
    12841 gtacgatgtg ggcggcaata cgggtaagtg ggctttgcgc tgctgcaaat acaatgaaaa
    12901 catcgccgtt acgttattag atctgccaca acaaatcgta ctggcgaaag aaaatatcgc
    12961 gaatgcaggt ttttctgatc gcatagattt ccaagcggtg gatatgctaa gcgatgcccc
    13021 gttaccaggc gaggctgata tctggtggat gagccaattt ttagactgtt tttcacccga
    13081 acaaataatt agcatactca gcaaaatcgc cagcgtaatg aagcctggcg caaaagtttg
    13141 catcatggag ttgttctggg atgcgcagcg gtttgaagca gcatcgttta gcctgaacgc
    13201 gtcctcactt tattttacct gcatggcaaa cggtaacagc cgtttctata gtgcagagaa
    13261 attttacgac tatctgaata aagcaggatt ccaggtagca gagcgccacg ataatttagg
    13321 cgttggacat acacttttga tatgccaaaa gaaataatcg tcacttaata attttggttt
    13381 accgcgggca cgcgttgatg aaatttgcat taaatattac caactggcag gcgctggcgc
    13441 ctggacttag cgatgtgcag caatggcaag catggtcgcg ccagccgtgg gcgattgacc
    13501 ctgccgcgcc gctggcaaaa ttaagtgaac tgccaatgat gactgcccgt cgtctcagtt
    13561 caggcagcaa actggctgtt gaatgcggtc tggcaatgct acgccgctat cagcctgacg
    13621 ccgttttgta taccagtcgt catggcgaac tggagcgtaa ttaccgcatt gttcatgcac
    13681 tggcaaccga acaggcgctt tctccgacgg acttcgcgct ttccgtacac aattcttccg
    13741 tgggtaacct gaccattgcg gccaaacagc cgatcgtttc gtcatcgctt tcagctggtc
    13801 gcgacagttt ccagcaaggt ttgtgtgaag tgcttagcct gctacaagcg ggataccagc
    13861 gcgtgttgat ggtcgatttt gacggttttc tgcccgagtt ttatcaccca caactgcctg
    13921 cagaaatgcc gacctggccg tacgccgtcg cgctgatgat tgaatctggc gatgactggc
    13981 aatgtgaaac gcagtcagcc attgcggtaa atgaaacaac actgccgcaa agcatgttgt
    14041 ttttacagca ctatttacaa aatgcaggcg cattttcact gtctggcgag cgcgtgcaat
    14101 ggcgctggag ccgcagatga ataagctgat gtcgcgcctg ggatgggcat ggcgactggt
    14161 catgaccggg ttatgttttg ccctgtttgg tctcggcggc ctgttgcttt cagtcgtatg
    14221 gtttaacata ttgttagttt tggtatggga tacttcccgc cgccgtcgtt tggcgcgtcg
    14281 cagcattgcc gccagttttc gcctgttttt aactgtcgcg aaagggcttg gcgtgctgga
    14341 ttatcgtatt gatggcgctg aaattttacg tcaggaacgc ggttgcctcg tcgtagccaa
    14401 tcatcccacg ctgatcgatt acgtgctgct ggcgtcagtc atgccagaaa ctgactgcct
    14461 ggtgaaaagc gcgctgttaa aaaatccttt tcttggcggc gtcgtgcggg cggcagatta
    14521 tttaatcaac agcgaagctg aaaccctgtt accccgctgc caacagcgac tggcacaggg
    14581 tgacactatc ctgattttcc ccgaaggcac acgaacgaaa cccggtgaaa aaatgacatt
    14641 acagcgcggt gcagcgaata ttgccgtgcg ctgcgccagc gatttacgca tcgtgacaat
    14701 cggctgtagt gagcatctgc tggataaaca aagtaaatgg tatgacgttc cgcctgccag
    14761 gccgtttttt acggttgaag tccatgggcg ggtagaaatc aataaatttt acgatgcaac
    14821 ttcacatgag ccggcgctgg ctgcaaggca gttaaaccga catcttttgc ttcaattaca
    14881 acaaggttgt ttacctctct caggaataaa taatgcaagc gctttatctg gaaattaaaa
    14941 atctcatcat cagcaccctc aatctggacg agttgactcc tgatgatatt gataccaatg
    15001 cgccactgtt tggcgacggt ttagggctgg actctatcga tgcactggaa ctggggctgg
    15061 cagtaaaaaa tgagtacggc gtcgtgctct ctgcggaaag cgaagaaatg cgtcagcact
    15121 ttttctccgt cgccactctg gcctctttca ttgctgcgca acgtgcctga ggaaagccat
    15181 catgacagac caacaaacca tttatcagga agtctcagcc ctgctggtta agctgtttga
    15241 aatcgatccg caggacatca aacctgaagc gcgcctgtac gaagatctgg aactggacag
    15301 catcgacgcc gttgacatga ttgtgcacct gcaaaagaaa accggtaaga aaatcaagcc
    15361 ggaagagttt aaagcagtgc gtacagtcca ggatgttgta gaggctgtaa aacgcctgct
    15421 acaagaaggc taatttgatg tcgggcattc gctcgctacc gatgattaag ttgctgaccg
    15481 gattattgct gctcgcctgg ccttttttaa tctggctcgg tttggcgcat aacagcctgc
    15541 actggctgtc gccgctgatg gcgttgctgc ttttattgcg cctgcgccag ccccgtcgtc
    15601 aggcagggcc gctacaagcc gtcacaaaaa tcgtcgctgt tgtgggcatt gtattgtgtg
    15661 tctccagttt tctgttgaaa acgcaccagt tactgctgtt ttacccggta gtggttaacg
    15721 ctgtcatgct ggcggttttt ggcggttcgc tgcggtcgac aatgccgata gttgagcggc
    15781 tggcacgttt gcaggaacca gacctcccgg aaaaggccgt gcgatatacc cgccgcgtaa
    15841 cgcaggtctg gtgcctgttt tttattttca acggcagtat tgcgttattt accaccttgt
    15901 atggcaacat gcctttgtgg acagcctgga acggtatcat tgcttacctt ctgataggtt
    15961 tcctgactgc aggggaatgg ctgatacgtt gtcagatgat caaacggaaa acaccatgaa
    16021 tcagactctc tcactttcac agtggttgac agcctctcgc cctgtaacaa ctcctgttgc
    16081 ctggcttggg gaatatacct ggacactggg gcatctgcgg catgatgtag cgcttttgat
    16141 cgaccattta cgcgaccagc caggcaatcg ctgggcactt tgttttgaaa acagctatct
    16201 ttttattgta gcgttgttgg ccacgctaca ctcaggtaaa atcccagtaa tcccgggcca
    16261 taatcgcgcc gctttgttaa atgtacaacg ctccttgttt gattgcatcc tgagtgataa
    16321 aaggctgggc tggaacggtc cacagtttgt tgtaagcaca tctcaccaga tgacgaccag
    16381 cacgatagcc tttgatgata ttgcttctga cgcgtttatt gaacttttca catcgggttc
    16441 aaccggacaa ccaaaacaaa ttgcaaaacc tgttatcagc cttgatcaag aagcaaacat
    16501 gctggcaact cactttgcag accgactact gggctgtcgt tttgtagcat ccgtgatgcc
    16561 tcagcacctg tatgggctga ctttccgtat ttttttacct atggcacttg gcctgccatt
    16621 gcatgccgca atgctctggt attctgagca acttgccgca ttaagccata cctaccgcta
    16681 tgcctttgtc agcagcccgg cgttccttaa acgactcgac cttcatctga cgcctccccc
    16741 cgtggaaatg attctctctg ccggcggtat gctaccatgg caggatgtag agaaaacttc
    16801 cacctggctg aatatctggc ccgatgaaat ctatggtagt accgaaacag gtattctcgc
    16861 atggcgttac cgtcaacagg ataatatccc ctggtttact ttttctgatg ttcatctttc
    16921 acaggaaagt gagggggttc gtgtcttttc gccactcatc cctgctgaag gattggtgct
    16981 tgacgatatg ttgcaattca atgaaaacgg tcagttccac ctgatcggac gacgtggtcg
    17041 agtggtaaaa atagaggaga agcggatatc actggttgaa atcgaacaac ggcttctggc
    17101 acttgacggt attatggatg tcgctgcaat accattaata cgcaatggcc gccaggctat
    17161 cggtgtcgtg gttgtaccaa ataaagaggc ccgtcaaata tggcaatgct ccggagggaa
    17221 aactctggag ttgtcctggc gcaaagcact attaccatgg cttgaacctg ttgcagtacc
    17281 tcgttactgg cgcattattg atgaaatacc agtaaacaat atgaataagc gtgtctatgc
    17341 acaattacag gagttatttc atgatacgcc atgaaattga gcgccatcag gttcaactgc
    17401 aggagatgga aatcgttcta taccttgacc cgatgctcta ctggtttaat ggtcattttg
    17461 ccgtacagcc tctgttacca ggcgtggccc agttggactg ggtaatgcat tatgctacca
    17521 cgctgcttgc tccgggctgg cgttttcgta gtatccagaa tgttaaattt ctggcgccac
    17581 ttatacctga gacgactgtt accctgcaat taacatggca ggaaacaagt caggtactca
    17641 ctttctgcta tcagcgtcac gacggtgacg cccgccacac cgccagtagc gggaagatcc
    17701 gcctatgtcg gtaaactttt ctccctgcgt gttgatcccc tgctacaacc acggtgcgat
    17761 gatgccgggc gtgctggcgc gtcttaagcc atttaatctt ccctgtattg tggtggatga
    17821 cggcagcgat gccgccacgc aacagcaact ggataatctg gttgccgaac agcctggcgt
    17881 gaccttaatt cgcctggcag aaaacacagg caaaggcgcg gcggtaatgc gcggcttaca
    17941 ggcggcggca gacgcgggct tcagccatgc ggtgcaggtg gatgctgacg gtcaacatgc
    18001 gattgaagat atccccaaac tgctggcact cgctgaacaa caccctgcgg cgctgatctc
    18061 cggccagcca atttacgatg actccatccc ccgctcacgg ctttacgggc gctgggtcac
    18121 ccacgtctgg gtatggatcg aaacgctctc cctgcaactg aaagacagta tgtgcggctt
    18181 tcgcgtttat ccggttgcgc caacgctgca actggcaaaa tacgccacca tcggcaagcg
    18241 gatggatttc gacactgaag tgatggtgcg cctctactgg cagggaaaca cctgttattt
    18301 cgtgccaacc cgcgtcactt atccgcagga cgggctttcg cattttgatg ccctgaaaga
    18361 taacgtccgc atctcgctca tgcacacgcg tctgtttttc ggcatgttgc cgcgtattcc
    18421 ttcactactg atgcgccgct cttccagcca ctgggcgcgg cagagtgaag tgaaaggatt
    18481 atggggaatg cgcctgatgc tgctggtctg gcgtctgctg ggaagaacgg cgtttagcgc
    18541 gctgctttac ccggtggtgg gcgtctactg gctcactgct gctcgtgcgc gcaaagcgtc
    18601 gcaagactgg ctcgcccgtg tacgacagca tcaaccacag gcggcaaaac tcaacagcta
    18661 tcagcacttt ctacgtttcg gtaatgccat gctcgacaaa atcgccagct ggcgcggcga
    18721 gctacaactg gggcacgatg tgctgtttgc gccaggcgca gaagcggcgc ttgacgtccg
    18781 cgatccgcgc ggcaaattgc tgctggcctc gcatcttggc gatgtggaag tgtgccgggc
    18841 gctggcaaaa attcagggct acaaaaccat taacgcgctg gtgtttagcg aaaacgccca
    18901 acgctttaaa cagataatgc aggagatggc tccccaggca ggcattaacc tgatgccggt
    18961 aacagatatc ggcccggaaa ccgccatcct gctgaaagag aagctggata acggcgaatg
    19021 ggtggcgatt gtcggtgacc gcatcgccgt caccccgcaa cgcggcggcg actggcgcgt
    19081 ctgctggagt ccgtttatgg gccagcctgc gcctttccca caggggccgt ttattctcgc
    19141 gtctattttg cgctgcccgg tgaatctgat tttcgccctg cgccagcgcg gcaagctgca
    19201 tattcactgc gaaacctttg ccgacccgct gctgttgccg cgtggcgaac gccaacaggg
    19261 gctgcaaaac gctatcgacc actacgccgc gcgtctggaa cattacgcgc tccagtcgcc
    19321 cctcgactgg tttaattttt tcgatttctg gcaactgccg gaaattcagg acaaggagta
    19381 aagggtgctt aacgatcccc gatttaccgc tgaagtcgag ctgaccattc cctttcacga
    19441 cgtcgatatg atgggcgtgg cctggcacgg taactatttt cgctactttg aagtggcccg
    19501 cgaggcgctg ctcaaccagt tcaattacgg ctatcggcag atgaaagagt caggttattt
    19561 gtggccagtg gtcgatgcgc gggtgaaata tcgccacgcc ctcacctttg agcaacgcat
    19621 ccgcgtgcgt gcgcatatcg aagagttcga aaaccgtctg cggattggct atcagatttt
    19681 cgatgccgaa acgggcaaac gcgccaccac aggatacacc attcaggtcg cagtagagga
    19741 gcaaagccgt gaactgtgct ttgtcagccc cgatattctg tttgaacgca tgggagtcaa
    19801 accatgaaat ttttaccgct gctggcgctg ctgattagcc cgtttgtgag cgccctgacc
    19861 ctggacgatc ttcagcaacg ctttaccgaa caaccggtga tccgcgccca tttcgatcaa
    19921 acccggacga ttaaagatct gccgcagccg ctgcgatctc agggtcagat gttgatcgcc
    19981 cgcgaccagg ggctgttgtg ggatcaaact tcaccattcc ccatgcagct attgctcgac
    20041 gataaacgca tggtgcaggt gatcaacggc cagccgccgc aaatcatcac ggcagaaaat
    20101 aacccgcaga tgttccagtt taaccacctg ctgcgcgcgc tgttccaggc cgatcgcaaa
    20161 gtgctggaac aaaacttccg cgtcgaattt gccgataaag gcgaaggccg ctggacgctg
    20221 cgcctgacgc cgaccaccac gccgctggat aaaattttca acaccatcga tctcgccggg
    20281 caaacctatc tggagagcat tcagcttaat gataaacagg gcgatcgcac cgatatcgct
    20341 cttacccaac atcaactgac gccagcgcaa ctgagcgatg acgaacgcca acgttttgcc
    20401 gcccagtaaa cgccccgcgc tgttatgggg gctagtctgc ctggtcatgg cggtggtatt
    20461 gctgatcctg ctgccgcaat cacggctgaa cagtagcgtg ctggctatgt tacccaaaca
    20521 ggcgatgggc gatattcccc cagcgctgaa tgacggcttt atgcagcgtc tggaccgcca
    20581 actggtgtgg ctggtcagcc ccggtaaaga ggctaatccc agggtggctc aggaatggct
    20641 gacgctgctg caaaagtccg ctgcgcttgg cgacattaaa gggccaatgg atgccgccag
    20701 ccagcaggcg tggggggcgt ttttctggca gcaccgcaac ggcctgattg accccgacac
    20761 ccgcgcccgc ctgcaaaacg gcggcgaagc gcaggcacaa tggatcctct cccagcttta
    20821 ttctgcgttc tccggggtaa gcggcaagga gttgcaaaac gatccgctga tgttaatgcg
    20881 cggctcgcag ctggcgatgg cgaaaaacgg ccagcgttta cgactgatgg acggctggct
    20941 ggtgacgcag gatccccagg gcaactactg gtatctgctg cacggcgaac tggcgggatc
    21001 gtcgtttgat atgcagcaaa cccaccagct catcacgacc ctgaatacgc tggaaaaaga
    21061 tctgaaaacg cgttacccgc aggcgcagtt gctctcgcgc ggcacggtgt tttacagcga
    21121 ttacgccagc caacaggcga agcaggatat ctccaccctg ggcgtggcaa cgctgctggg
    21181 ggtgatattg atgattgtgg cggtgttccg ctctttacgc ccgttgctgc tttgcgtgat
    21241 ttccatcggc atcggcgcgc tggcgggaac ggccgccaca ttgttgattt tcggcgaatt
    21301 acacctgatg acgctggtga tgagcatgag cgttatcggc atttccgctg actacacgct
    21361 ctattacctc accgaacgga tggtgcacgg caacgacgtt tcgccgtggc aaagcctggc
    21421 gaaagtgcgc aatgccttgc tactggcgct gctcaccacc gtggcggcat atctgattat
    21481 gatgctcgcc ccttttcccg gtattcggca aatggcgatt tttgccgccg tcgggttgag
    21541 cgcctcctgt ctgaccgtcc tgttctggca tccgtggctg tgccgtggcc tgccggtacg
    21601 cccggttccg gcgatggcgc tgatgctgcg ctggctggcg gcgtggcggc gtaataaaaa
    21661 actgtcgctg ggtctgcccg tcgcgctggc gctgttttcg ctggcgggga tgtcaatgct
    21721 acgcgtcgat gacgatatct cgcagttaca ggcgctgccg cagcatattc tggcgcagga
    21781 gaaagccatt accgccctga ccggacaaag cgtcgatcaa aaatggtttg tagtttacgg
    21841 cgactcccca cagcaaacct tgggacggct ggagacattt accacctcgc tggagaaagc
    21901 gaaaaaagac ggccttatca gcaactaccg caccattccg ctgaactccc tggcgcggca
    21961 ggaggaagat ttagacctgc tgaaaacggc tgccccgacg gtaacaaaag cactgcaaaa
    22021 tgccgggctg acggcggtga acccggatct taacgccatg ccagtgaagg ttgatgaatg
    22081 gctggcaagc cccgccagtg aaggctggcg tctgctgtgg ctgacgctgg aaaacggcga
    22141 aagcggcgta ctggtgccgg ttgaaggggt taaaagtagc gcgttgcagc aggaaatcgc
    22201 cagctattac cctggcggca ttgcctgggt tgatcgcaaa agcacctttg atgaattgtt
    22261 cgcactttac cgttatgtgt taaccggctt gttgctggtg gcgctggcag taattgcctg
    22321 cggcgcagtg gcccgcctcg gctggcgcaa agggcttatc agcctggtgc cttcggtgct
    22381 ttcgctgggc tgcggtctgg cagtgctggc gatgagcggg caggcggtga atctcttttc
    22441 gctactggcg ctggtgctgg tgcttggcat cggcatcaat tacacgctgt ttttcagtaa
    22501 tccgcgcggt acaccgttaa cttccctact ggcgatcacg ctggcaatgc tcaccacctt
    22561 gctgacgctg ggaatgctgg tattcagcgc cacccaggcc atcagcagtt ttggtattgt
    22621 gctggtgagc ggtattttca ccgccttcct gctttcgccg ctggctatgc ccgataaaaa
    22681 gagaacaaaa aaatgatcaa atccaccttc tggcgagcgc tcgccctgac cgctacgctt
    22741 atcctcaccg gctgtagcca ctcgcaaccg gaacaggaag gccgcccgca ggcgtggctg
    22801 caacctggca cacgcatcac gctgcctgcg ccggggattt cacccgccgt caattcccag
    22861 caactcttga gcggcagctt caacggcaaa acccagtcgc tgctagtgat gcttaacgcc
    22921 gacgaccaga aaatcaccct tgccgggctg tcgtcggtcg gcattcgcct gtttctggta
    22981 acctacgatg caaaaggtct acgcgccgag caatccatcg tcgtcccgca gctgccgccc
    23041 gcaagtcagg tgctggctga cgtaatgctc agccactggc cgattagcgc ctggcaaccg
    23101 caacttccca ctggctggac gcttcgcgac aacggcaaca aacgcgagct gcgtaacgcc
    23161 agcggcaagc tggtcacgga aatcatctat ctgaatcgcc agggcaaacg cgtgccaatc
    23221 agcattgagc agcatgtctt taaataccac atcaccattc aatacttagg tgactgatat
    23281 gatttatatt tccgccgtcg gcatgatcaa cgccctggga aataatcttg acgaaatcgc
    23341 agccaacctg gctcgcggtg tcgcccccgg catgcgtccg cgcgcaggct ggttgcaggg
    23401 gcatccgcag gcggtcctgg ctggcgtgga tggcgagctt ccgcttatcc ccgaaaaatt
    23461 cgctgcgcac cgctcgcgca acaatcagat cctgctggcg gcgctggcgc agatccagcc
    23521 gcaggttgat gacgctatcg cgaaatatgg ccgcgagcgc atcgcgattg tgcttggcac
    23581 cagcacctcc ggcctgcatg agggcgacac gcacgttaac ctgcgcacca acggacagcc
    23641 gagcaccaca tggcactacg cgcaacagga actcggcgat ccgtcgcgtt ttctcagcca
    23701 ctggctggcg ctcgacggcc cggcctatac cctttcaacc gcctgctctt ccagcgccag
    23761 agcgatgatc agcgggcgca ggcttatcga agcggggctg gtcgatgccg ccattgtggg
    23821 gggcgcagat actctgagcc ggatgccgat taacggtttt cacagcctcg aatcgctctc
    23881 gccaacctta tgtcagccgt ttggtcgtga ccgcgcaggt atcaccattg gcgaaggggc
    23941 gggactgatg ttattaaccc gtgaaccgca gcccatcgcg ttactgggcg ttggtgaatc
    24001 gagcgacgct taccatattt cggccccgca tccgcaaggc gaaggggcga tccgcgccat
    24061 taaccaggcg ctgaccgatg cgcagcttac gccagatgac atcggctata tcaacctgca
    24121 cggtaccgcc acacaactca acgaccagat tgaatcaatg gtggttaacg ccctgtttgg
    24181 caaacgcata ccgtgcagct ccaccaaaca tttgaccgga cacacgctcg gcgcggctgg
    24241 cattaccgag gcggcaatta gtatgttgat tttacagcgt gatttaccgc tgcccgctca
    24301 ggacttcagc ctgtcgccgc gcgatcccac gctgccaccg tgtggcatta tcgaaaaacc
    24361 gcagccgctg gcacgtccgg tgatcctgtc caattccttc gcctttggcg gcaataacgc
    24421 cagcattctg ctcgggaggg tttcgtgagc cactatttat cccccggcgc ttatctgccc
    24481 cacgacgcgc ccatgttact gctggaagac gtggtgagcg tcagcgatga cagcgcggtg
    24541 tgccgcgtga cggtttcgcc cagtggcgta ctggcaccgt ttctcgaccc ggacggcaat
    24601 ctccctggct ggtttgccct tgaactgatg gcgcaaaccg tcggcgtctg gtctggctgg
    24661 catcgccacc agcagggcaa aaacagtatt gagttaggga tgatactggg cgcgcgtgaa
    24721 ttgctttgca ccactggcat tctgcccgca ggccagacgc tgaccattac cgtcaaactg
    24781 ctgatgcagg acgatcgctt cggcagcttt gagtgcagca tcaacgacgg cgaggcgacg
    24841 ggccgcatta acaccttcca gccgaccgcg gaagaactta ccaccctgtt tcaacaagga
    24901 gcgtcctgat gagtcgttca gttctggtta ctggtgccag caaaggcatt ggtcgcgcca
    24961 tcgcctgcaa gcttgcggca gacggcttta acattggcgt tcactatcac cgtgacgcca
    25021 caggcgctca ggaaacgctg aatgccattg tcgccaacgg gggtaacggg cgtttgctca
    25081 gtttcgacgt cgcaaaccgc gaacagtgtc gggaagtgct ggagcatgag atcgcgcaac
    25141 acggtgcctg gtacggcgtg gtcagtaacg ccgggatcgc ccgcgatgcc gcttttccgg
    25201 ctttaagcga tgacgactgg gatgcggtga tccacaccaa cctcgacagt ttctacaacg
    25261 tcattcagcc gtgcattatg ccaatgatcg gcgcgcgcca gggtgggcgc atcatcacgc
    25321 tgtcctcggt ttccggcgta atgggcaatc gcggccaggt gaattacagc gcagccaaag
    25381 ccggaattat cggcgcaaca aaagcactgg ctatcgaact cgcaaagcgc aaaatcaccg
    25441 tcaattgcat cgcgccaggg cttattgata ccgggatgat cgaaatggaa gaaagcgctc
    25501 tgaaagaggc aatgtcgatg atccccatga aacgtatggg ccaggcggaa gaagttgccg
    25561 ggcttgccag ttatttaatg tctgatattg cgggttacgt cacccgccag gttatttcca
    25621 tcaacggagg gatgttatga cacgtcgcgt agtgattaca ggaatgggcg gcgtgactgc
    25681 ctttggtgaa aactggcagg acgtttctgc acgcttactg gcgtatgaaa atgcggtgcg
    25741 caaaatgccg gagtggcagg tctacgatgg gctgcacacc ctgcttggcg cgcctgtgga
    25801 tgatttcacg ctaccggagc actacacccg caagcgtatc cgtgcgatgg gccgcgtgtc
    25861 gcaaatgtcg acccgtgcca gcgagctggc gttagagcag gcagggttga ttggcgatcc
    25921 gatattaacc agcggcgaaa cgggcattgc gtacggttca tcgaccggca gtaccggtcc
    25981 ggtgagcgaa tttgccacca tgctgactga aaagcacacg aacaacatca ccggcaccac
    26041 ctatgtgcag atgatgccgc acaccaccgc tgttaatact ggcctctttt tcggcctgcg
    26101 cgggcgggtg atcccaacct ccagcgcctg tacctccggc agccaggcga tcggttatgc
    26161 gtgggaagcc attcgccacg gttatcaaac ggtgatggtt gccggtggtg ccgaagagtt
    26221 atgcccttca gaagcagcgg tgtttgatac cctttttgcc accagccagc acaatgatgc
    26281 gccgaaaacc acaccttcac cgttcgatga aaaccgcgac ggactggtga ttggtgaagg
    26341 cgcaggtacg ctcattctcg aagaactgga gcatgccaaa gcgcgcagcg cgaccattta
    26401 cggtgaaatc gtcggctttg cgactaactg cgacgcggca catattaccc agccgcagcg
    26461 cgaaaccatg cagtattgta tggagcaatc gctaaaaatc gcagggttaa gcgcacaaga
    26521 tatcggctat atttcagcgc atggtacggc gaccgatcgc ggtgatatgg cagaaagtct
    26581 ggcgacagca accatttacg gtgataatgt gccgctctcc tcgctgaaaa gttattttgg
    26641 tcatacgctt ggtgcctgtg gcgcgctcga agcctggatg agtctgcaaa tgatgcgcga
    26701 aggttggttt gcgcctacgc taaacctgaa taaaccagac ccgaattgtg gcgcgctgga
    26761 ttacatcatg catgaagccc gcaaggttga ttgtgagttt ttgcagagta acaactttgc
    26821 ctttggcggc attaatacat ccatcattat taagcgctgg ccctgagtta tacaaccaca
    26881 tggacatccc atgtggtgat tcttcaaaaa atctgaatca atgaatatga acctcaaatt
    26941 ctcttacgca tactgaagag cattccgtat aacgattcag cagacaaaac tttatcagtc
    27001 cacacgaatc aacggatgca cagatgacac gcccggttat caccgggcaa tggttgccat
    27061 aacgggtaac ataagggact gaacagttgt ctttagcgca acgaactgat ctaacgtacc
    27121 tgaacggaca acctgaaaac agccttccat cctgcattgt ccgccagtgc ttattaccaa
    27181 ccacgtctgt tgcagtaaag taatcagttg cgaacattat ttatcaatcg tgacgctggc
    27241 tcgcgcatgg cagaaaaggt cctgactgag caacaacagg catcgtactg ttgctgctcc
    27301 atgttgttta tatgaacgca tcccgtttgc aaggcttccg tgtttttgac gttgacggta
    27361 gattgctgct ctatatcctt attgcagccg gtgctgcggg cctcaatgag attcgctgac
    27421 tctcgcctta ttcacgcaac gagctttaag ctcagaaaaa catccaagtt tggtgagtca
    27481 cagtcttacc tgtcgttgtc atcaatatag gaatggataa tctggagagc gatactgcag
    27541 gctgatattg cggttttgcg acaagttttt agcatccgct tctacgccaa atacctgaag
    27601 taggaatgga tgcgttagca gcatacatcc ggaactgcgc ggggaatagc cggaaacggc
    27661 tatccctacc acaatagcta actttgtaat agcgctcttt taggctcatg tactctgacg
    27721 taattctatc tggtagttga attgcacgat acgcgactct tctgattcat catgattatt
    27781 attcaatagt ccaatcagta acttacctgt ctctatcccc atttttttgt agtcaatacg
    27841 gatggatgtc aaagttgggt gtgtttgatc gcaattgtct gaaccgtcaa gacaagctac
    27901 agcgatattt ccagggattt ttaacaaacg ccgctggcac tcaaacatta tacccagcgc
    27961 aatctcttcg tggctgcaaa tcacagcatc cagctctggt tgtcgcagta atatctcgtt
    28021 taatgcgtaa cgccccaatt gcagacttgc ggcatccggc atggtgataa caagatcagt
    28081 gttctggtaa tggctcagca ttgctttatg ccagccgttc agttgctgac gttgcaggcg
    28141 gttgtccata tgcgcaccga taaatccgat attcttataa ccctgctcca gcagatgttc
    28201 ggtcagttta tgtgatgctt caaaaaatgc tgtttcgatg gtaatttgag ccggactgaa
    28261 aggggaaccg ataacgttaa tggtaggtat atttgttttc tcgagtatct ggtgagtttt
    28321 atcagccaac tgagaaccaa aaataactaa cgccgccggg ttgccttgaa gaagcgtcat
    28381 cagaatttca gcttctttga gctgattata ctcatgacac ccaagcagta gctggaattc
    28441 atttttatta agcacctgct gtaatgactg cataaagcgg gagctggctt tatctgtcag
    28501 ggaaggaacc agtacggcga ctgtat
//
LOCUS       DAZGAP010000048        28352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGAP010000048 DAZGAP010000000
VERSION     DAZGAP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.048"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..1184
                     /gene="mltB"
                     /locus_tag="ACHLAM_001738"
     CDS             99..1184
                     /gene="mltB"
                     /locus_tag="ACHLAM_001738"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO7853278.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            1329..1826
                     /gene="pncC"
                     /locus_tag="ACHLAM_001739"
     CDS             1329..1826
                     /gene="pncC"
                     /locus_tag="ACHLAM_001739"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO7853279.1"
                     /translation="MTDSELMQLSEQVGQSLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            1906..2967
                     /gene="recA"
                     /locus_tag="ACHLAM_001740"
     CDS             1906..2967
                     /gene="recA"
                     /locus_tag="ACHLAM_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO7853280.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            3036..3536
                     /gene="recX"
                     /locus_tag="ACHLAM_001741"
     CDS             3036..3536
                     /gene="recX"
                     /locus_tag="ACHLAM_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO7853281.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            3664..6294
                     /gene="alaS"
                     /locus_tag="ACHLAM_001742"
     CDS             3664..6294
                     /gene="alaS"
                     /locus_tag="ACHLAM_001742"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO7853282.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVNSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            6529..6714
                     /gene="csrA"
                     /locus_tag="ACHLAM_001743"
     CDS             6529..6714
                     /gene="csrA"
                     /locus_tag="ACHLAM_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO7853283.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            7030..7122
                     /locus_tag="ACHLAM_001744"
     tRNA            7030..7122
                     /locus_tag="ACHLAM_001744"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7064..7066,aa:Ser,seq:gct)
     gene            7126..7202
                     /locus_tag="ACHLAM_001745"
     tRNA            7126..7202
                     /locus_tag="ACHLAM_001745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7160..7162,aa:Arg,seq:acg)
     gene            7265..7341
                     /locus_tag="ACHLAM_001746"
     tRNA            7265..7341
                     /locus_tag="ACHLAM_001746"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7299..7301,aa:Arg,seq:acg)
     gene            7405..7481
                     /locus_tag="ACHLAM_001747"
     tRNA            7405..7481
                     /locus_tag="ACHLAM_001747"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7439..7441,aa:Arg,seq:acg)
     gene            7762..8328
                     /gene="yqaB"
                     /locus_tag="ACHLAM_001748"
     CDS             7762..8328
                     /gene="yqaB"
                     /locus_tag="ACHLAM_001748"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO7853284.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            8325..8753
                     /locus_tag="ACHLAM_001749"
     CDS             8325..8753
                     /locus_tag="ACHLAM_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO7853285.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            8826..10382
                     /gene="gshA"
                     /locus_tag="ACHLAM_001750"
     CDS             8826..10382
                     /gene="gshA"
                     /locus_tag="ACHLAM_001750"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO7853286.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFIAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            10532..11047
                     /gene="luxS"
                     /locus_tag="ACHLAM_001751"
     CDS             10532..11047
                     /gene="luxS"
                     /locus_tag="ACHLAM_001751"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO7853287.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(11098..12210)
                     /locus_tag="ACHLAM_001752"
     CDS             complement(11098..12210)
                     /locus_tag="ACHLAM_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7853288.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALVTQPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(12207..12914)
                     /locus_tag="ACHLAM_001753"
     CDS             complement(12207..12914)
                     /locus_tag="ACHLAM_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFO7853289.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFDYGEQS"
     gene            complement(13172..14710)
                     /gene="emrB"
                     /locus_tag="ACHLAM_001754"
     CDS             complement(13172..14710)
                     /gene="emrB"
                     /locus_tag="ACHLAM_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO7853290.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14727..15899)
                     /gene="emrA"
                     /locus_tag="ACHLAM_001755"
     CDS             complement(14727..15899)
                     /gene="emrA"
                     /locus_tag="ACHLAM_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO7853291.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(16026..16556)
                     /gene="emrR"
                     /locus_tag="ACHLAM_001756"
     CDS             complement(16026..16556)
                     /gene="emrR"
                     /locus_tag="ACHLAM_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO7853292.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(16647..16982)
                     /gene="ygaH"
                     /locus_tag="ACHLAM_001757"
     CDS             complement(16647..16982)
                     /gene="ygaH"
                     /locus_tag="ACHLAM_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO7853293.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(16972..17709)
                     /gene="ygaZ"
                     /locus_tag="ACHLAM_001758"
     CDS             complement(16972..17709)
                     /gene="ygaZ"
                     /locus_tag="ACHLAM_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO7853294.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(17833..19017)
                     /locus_tag="ACHLAM_001759"
     CDS             complement(17833..19017)
                     /locus_tag="ACHLAM_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7853295.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRTL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLPWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASVWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AI"
     gene            complement(19209..20201)
                     /gene="proX"
                     /locus_tag="ACHLAM_001760"
     CDS             complement(19209..20201)
                     /gene="proX"
                     /locus_tag="ACHLAM_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO7853296.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(20259..21323)
                     /gene="proW"
                     /locus_tag="ACHLAM_001761"
     CDS             complement(20259..21323)
                     /gene="proW"
                     /locus_tag="ACHLAM_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO7853297.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(21316..22518)
                     /gene="proV"
                     /locus_tag="ACHLAM_001762"
     CDS             complement(21316..22518)
                     /gene="proV"
                     /locus_tag="ACHLAM_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO7853298.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(22874..23833)
                     /gene="nrdF"
                     /locus_tag="ACHLAM_001763"
     CDS             complement(22874..23833)
                     /gene="nrdF"
                     /locus_tag="ACHLAM_001763"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7853299.1"
                     /translation="MKLSRISAINWNKITDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(23843..25987)
                     /gene="nrdE"
                     /locus_tag="ACHLAM_001764"
     CDS             complement(23843..25987)
                     /gene="nrdE"
                     /locus_tag="ACHLAM_001764"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7853300.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSSDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSSITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(25960..26370)
                     /gene="nrdI"
                     /locus_tag="ACHLAM_001765"
     CDS             complement(25960..26370)
                     /gene="nrdI"
                     /locus_tag="ACHLAM_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO7853301.1"
                     /translation="MSQIVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(26367..26612)
                     /gene="nrdH"
                     /locus_tag="ACHLAM_001766"
     CDS             complement(26367..26612)
                     /gene="nrdH"
                     /locus_tag="ACHLAM_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO7853302.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAANA"
     gene            complement(26859..27188)
                     /locus_tag="ACHLAM_001767"
     CDS             complement(26859..27188)
                     /locus_tag="ACHLAM_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO7853303.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            27340..27684
                     /locus_tag="ACHLAM_001768"
     CDS             27340..27684
                     /locus_tag="ACHLAM_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO7853304.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(27721..28170)
                     /gene="alaE"
                     /locus_tag="ACHLAM_001769"
     CDS             complement(27721..28170)
                     /gene="alaE"
                     /locus_tag="ACHLAM_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO7853305.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         7003 a         7115 c         7537 g         6697 t
ORIGIN      
        1 atgtaaagtc cgtatcgggc agtgactacc gcttccttgt gcggggcgtg atgctttacc
       61 atacttgccc ctggttgaat ctgttaaatg gacccctcat gttcaagcgt cgttatgtaa
      121 cattgcttcc cctttttgtg ttgcttgccg cctgtagcag caagccaaaa cctactgaga
      181 ctgaaacgac caccggaacg ccgtctggcg gcttcctgct tgagccgcag cacaatgtga
      241 tgcagatggg cggtgacttc gccaataatc cgaatgccca gcagttcatc gacaaaatgg
      301 tgaacaaaca cggtttcgat cgtcagcagt tgcaggaaat tctctcccag gcgaaacgtc
      361 tggattcggt actgcggctg atggataacc aggcaccaac cacatcggtg aaaccgccat
      421 caggtccgaa cggcgcatgg ctgcgttatc gcaaaaaatt tattacgccg gacaacgtgc
      481 agaacggtgt ggttttctgg aatcagtatg aagatgcgtt gaatcgcgcg tggcaggtgt
      541 atggtgtacc gccggaaatt atcgtcggga ttatcggcgt tgaaacccgc tgggggcgcg
      601 tgatggggaa aactcgcatc ctcgatgcgc tggcaacgct gtcatttaac tacccacgcc
      661 gcgcggagta tttttctggc gagctggaaa ccttcctgct gatggcgcgc gacgagcagg
      721 acgatccgct caatctgaaa ggttcctttg ccggggcgat gggctacgga cagtttatgc
      781 cgtcgtctta caaacaatat gcggtagatt tcagcggcga cgggcatatc aacctgtggg
      841 atccggttga tgccattggt agtgtggcga actatttcaa agcgcacggc tgggtgaaag
      901 gtgatcaggt tgcggtgatg gcaaacggtc aggctccagg cttgccaaat ggcttcaaaa
      961 ccaagtacag catttcgcag cttgccgcgg caggtttaac gccacagcag ccgctgggca
     1021 accatcaaca agccagcctg ctgcgtctgg atgttggcac cggctaccag tactggtacg
     1081 gtctgccgaa cttctacacc attacccgtt ataaccacag cacccattac gcaatggcgg
     1141 tctggcagtt aggacaagcc gtggcgctgg cgcgcgtaca gtaactattt gtaagagggg
     1201 ggcgtgagct tccctctggc taatcagctt ttttctgaat cctcctcgta aaattgcaac
     1261 gccaacacca tcttcctgac gaaagtgtta tcttgtccgg cataaatttt tgactgacag
     1321 aggttgtgat gactgacagt gaactgatgc agttaagcga acaggttggg cagtcgctga
     1381 aagcccgtgg cgcaaccgta acaactgctg agtcttgtac cggcggttgg gtagcgaaag
     1441 tgattaccga tattgccggc agttccgcct ggtttgaacg cggatttgtc acctacagta
     1501 acgaagcaaa agcgcaaatg atcggtgtac gcgaagagac gctggcacag catggcgcgg
     1561 tgagcgaacc tgtcgtggtg gaaatggtga taggcgcact gaaagcggct cgtgctgatt
     1621 acgccgtgtc tattagtggt atcgccgggc cggatggcgg cagtgaagag aaacctgtcg
     1681 gcaccgtctg gtttgctttt gccactgccc gtggtgaagg cattacccgg cgggaatgtt
     1741 tcagcggtga ccgtgatgcg gtgcgtcgtc aggctactgc gtatgcattg cagaccttgt
     1801 ggcaacaatt tctacaaaac acttgatact gtatgagcat acagtataat tgcttcaaca
     1861 gaacatattg actatccggt attacccggc atgacaggag taaaaatggc tatcgacgaa
     1921 aacaaacaga aagcgttggc ggcagcactg ggccagattg agaaacaatt tggtaaaggc
     1981 tccatcatgc gcctgggtga agaccgttcc atggatgtgg aaaccatctc taccggttcg
     2041 ctttcactgg atatcgcgct tggggcaggt ggtctgccga tgggccgtat cgtcgaaatc
     2101 tacggaccag aatcttccgg taaaaccacg ctgacgttgc aggtgatcgc cgcagcgcag
     2161 cgcgaaggta aaacctgtgc gtttatcgat gctgaacacg cgctggaccc aatctacgca
     2221 cgtaaactgg gcgttgatat cgacaacctg ctgtgctccc agccggatac cggcgagcag
     2281 gcactggaaa tctgtgacgc cctggcgcgt tctggcgcag tagacgttat cgtcgttgac
     2341 tccgtggcgg cactgacgcc gaaagcggaa atcgaaggcg aaatcggcga ctctcacatg
     2401 ggccttgcgg cacgtatgat gagccaggcg atgcgtaagc tggcgggtaa cctgaagcag
     2461 tccaacacgc tgctgatctt catcaaccag atccgtatga aaattggtgt aatgttcggt
     2521 aacccggaaa ccactaccgg tggtaacgcg ctgaaattct acgcctctgt tcgtctcgac
     2581 atccgtcgta tcggcgcggt gaaagagggc gaaaacgtgg tggggagcga aacccgtgtg
     2641 aaagtggtga agaacaaaat cgctgcgccg tttaaacagg ctgaattcca gatcctctac
     2701 ggcgaaggta tcaacttcta cggcgaactg gttgacctgg gcgtgaaaga gaagctgatc
     2761 gagaaagcag gcgcgtggta cagctacaaa ggtgagaaga tcggtcaagg taaagcgaat
     2821 gcaactgcct ggctgaaaga taacccggaa accgcgaaag agatcgagaa gaaagtacgt
     2881 gagttgctgc tgagcaaccc gaactcaacg ccggatttct ctgtagatga cagcgaaggc
     2941 gtagcagaaa ctaacgaaga tttttaatcg tcttgtttga tacacaaggg tcgcatctgc
     3001 ggcccttttg cttttttaag ttgtaaggat atgccatgac agaatcaacg tcccgtcgcc
     3061 cggcatatgc tcgcctgttg gatcgtgcgg tacgcattct ggcggtgcgc gatcacagtg
     3121 agcaagaatt gcgacgtaaa ctcgcggcac cgatcatggg caaaaatggc ccggaagaga
     3181 ttgatgccac ggcagaagat tacgagcgcg ttatcgcctg gtgccatgaa catggctatc
     3241 tcgatgacag ccgatttgtt gcgcgcttta tcgccagccg tagccgcaaa ggttatggac
     3301 ctgcgcgtat tcgtcaggaa ctgaatcaga aaggtatttc ccgcgaagcg acagaaaaag
     3361 cgatgcgtga atgtgacatc gactggtgcg cactggcgcg cgatcaggcg acgcgaaaat
     3421 atggcgaacc tttgccaact gtcttttcag aaaaagttaa gatccagcgt tttctgctct
     3481 atcgtggcta tctgatggaa gatatccagg atatttggcg aaattttgcc gactgaacgc
     3541 atacgggatt ttacttccca gtcaagaaaa cttatcttat tcccactttt cagttaccag
     3601 cccggcggtt aaaacacgct gtagctggtg gcgatatttc gttagcttga tttcaggata
     3661 attatgagca agagcaccgc tgagatccgt caggcgtttc tcgacttttt ccatagtaag
     3721 ggacatcagg tagttgccag cagctccctg gtaccccata acgacccaac tttgttgttt
     3781 accaacgccg ggatgaacca gttcaaggat gtgttccttg ggctcgacaa gcgtaattat
     3841 tcccgcgcta ccacttccca acgctgcgtg cgtgcgggtg gtaaacacaa cgacctggaa
     3901 aacgtcggtt acaccgcgcg tcaccatacc ttcttcgaaa tgctgggcaa cttcagcttc
     3961 ggcgactatt tcaaacacga tgccattcag tttgcatggg aactgctgac cagcgaaaaa
     4021 tggtttgccc tgccgaaaga gcgtctgtgg gttaccgtct atgaaagcga cgacgaagcc
     4081 tacgaaatct gggaaaaaga agtcgggatc ccgcgcgaac gtattattcg catcggcgat
     4141 aacaaaggtg cgccatacgc atctgacaac ttctggcaga tgggtgacac tggtccgtgc
     4201 ggcccgtgca ccgaaatctt ctacgatcac ggcgaccaca tttggggtgg ccctccggga
     4261 agtccggaag aagacggcga ccgctacatt gagatctgga acatcgtctt catgcagttc
     4321 aaccgccagg cagatggcac gatggaaccg ctgccgaagc cgtctgtaga taccggtatg
     4381 ggtctggagc gtattgctgc ggtgctgcaa cacgttaact ctaactatga catcgacctg
     4441 ttccgtacgc tgatccaggc ggtagcgaaa gtcactggcg cgaccgattt gagcaacaaa
     4501 tcgctgcgcg taatcgctga ccacattcgt tcttgtgcgt tcctgatcgc ggatggcgta
     4561 atgccgtcca acgaaaaccg tggttatgta ctgcgtcgta tcattcgtcg cgcagtgcgt
     4621 cacggcaata tgctgggcgc gaaagaaacc ttcttctaca aactggttgg tccgctgatc
     4681 gacgtaatgg gctctgcggg tgaagacctg aaacgccagc aggcgcaggt tgagcaggtg
     4741 ctgaagactg aagaagagca gtttgcccgt actctggagc gcggtctggc gctgctggac
     4801 gaagagctgg caaaactttc tggtgatacg ctggatggtg aaacggcttt ccgtctgtac
     4861 gacacctatg gcttcccggt tgacctgacg gctgatgttt gtcgtgagcg caacatcaaa
     4921 gttgacgaag ctggatttga agcagcaatg gaagagcagc gtcgtcgtgc gcgcgaagcc
     4981 agcggctttg gtgccgatta caacgcaatg atccgtgtta acagtgcatc tgaatttaaa
     5041 ggctatgacc atctggaact gaacggcaaa gtgaccgcgc tgtttgttga tggtaaagcg
     5101 gttgatgcca tcaatgcagg ccaggaagct gtggtcgtgc tggatcaaac gccattctat
     5161 gcggaatccg gcggtcaggt tggtgataaa ggcgaactga aaggcgctaa cttctccttt
     5221 gcggtggaag atacgcagaa atacggccag gcgattggtc acatcggtaa acttgctgcg
     5281 ggttctctga aagtgggcga cgcggtgcag gctgatgttg atgaagctcg tcgcgcccgt
     5341 attcgtctga atcactccgc aacgcacctg atgcacgctg cgctgcgcca ggttctgggt
     5401 actcatgtat cgcagaaagg ttcactggtt aacgacaaag tgctgcgctt cgacttctca
     5461 cacaacgaag cgatgaaacc agaagagatt cgtgcggtcg aagacctggt gaacgcacag
     5521 attcgccgta acttgccgat tgaaaccaac atcatggatc tcgaagcggc gaaagcgaaa
     5581 ggtgcgatgg cgctgttcgg cgagaagtat gatgaacgtg tacgtgtgct gagcatgggc
     5641 gatttctcca ccgagctgtg tggcggtact cacgccagtc gcactggtga tattggtctg
     5701 ttccgcatca tctctgaatc gggtactgct gcaggcgttc gtcgtatcga agcggtaacc
     5761 ggagaaggcg ctatcgccac cgttcatgca gacagcgatc gcttaaacga agtcgcgcat
     5821 ctgctgaaag gcgatagcaa taatctggct gataaagtgc gctcagtact ggaacgtacg
     5881 cgtcagctgg aaaaagagtt acaacagctt aaagaacaag ctgccgcaca ggagagcgca
     5941 aatctttcca gtaaggcaat tgatgttaat ggtgttaagc tgttggttag cgagcttagc
     6001 ggtgttgagc cgaaaatgtt gcgtaccatg gttgacgatt taaaaaatca gctggggtcg
     6061 acaattatcg tgctggcaac ggtagccgaa ggtaaggttt ctctgattgc aggcgtatct
     6121 aaggacgtca cagatcgtgt gaaagcaggg gaactgattg gtatggtcgc tcagcaggtg
     6181 ggcggcaagg gtggtggacg tcctgacatg gcgcaagccg gtggtacgga tgctgcggcc
     6241 ttacctgcag cgttagccag tgtgaaaggc tgggtcagcg cgaaattgca ataatataag
     6301 cgtcaggcaa tgccgtggac tcgcttcacg gcattcgcat taacgctatc gacaacgata
     6361 aagtcaggtt gaagttgtgt atatcggcta aacttaggtt taacagaatg taatgccatg
     6421 actgcttaga tgtaatgtgt ttgtcattgc ttactttttg gcgttatatg atggataatg
     6481 ccgggataca gagagacccg actcttttaa tctttcaagg agcaaagaat gctgattctg
     6541 actcgtcgag ttggtgagac cctcatgatt ggggatgagg tcaccgtgac agttttaggg
     6601 gtaaagggca accaggtacg tattggcgta aatgccccga aggaagtttc tgttcaccgt
     6661 gaagagatct accagcgtat ccaggctgaa aaatcccagc agtccagtta ctaatctttc
     6721 cgcgtctcat ctttatcggt gagacgcacc ctcaaaattt cttcttcact ccattgtctt
     6781 ttcgctttac tcccgttcat tcaacttaag tctccatttt tttgcattac tgctatctga
     6841 cagacctccg tttttctgtt gataaaacac tctttttgac gtttttacag actaattgag
     6901 cgtgaagtgt gcaaacgata aaagtgtggg aaaaattgtt tgacttataa gtctcagaaa
     6961 gtaatatgtg cgccacgcag cgacgatgag cgataaacaa gttcttcgaa gcactcgtaa
     7021 gaggcgtgtg gtgaggtggc cgagaggctg aaggcgctcc cctgctaagg gagtatgcgg
     7081 tcaaaagctg catccggggt tcgaatcccc gcctcaccgc catttgcatc cgtagctcag
     7141 ctggatagag tactcggcta cgaaccgagc ggtcggaggt tcgaatcctc ccggatgcac
     7201 catctcttac ttgatatggc tttagtagcg gtatcaatat cagcagtaaa ataaatttcc
     7261 cgatgcatcc gtagctcagc tggatagagt actcggctac gaaccgagcg gtcggaggtt
     7321 cgaatcctcc cggatgcacc atctcttact tgatatggct ttagtagcga tatcaatatc
     7381 agcagtaaaa taaattttcc caatgcatcc gtagctcagc tggatagagt actcggctac
     7441 gaaccgagcg gtcggaggtt cgaatcctcc cggatgcacc atattctccg taaccttcag
     7501 caatgaaggt atcgaagagg tagcggtatt aaccgcaggc accagggagg ataacgttgc
     7561 gccagcagcg gcccgaaggg cgaacgccag tgagtcatcc tcccggatgc accatcttct
     7621 ccaaaaaatt tccttcaaaa atatctcatt tctaaataac tactttatcc atttcctgaa
     7681 tattcttctc cttctccatc agcgacaaaa tcaagcaatt acgctaaagt agcgtcatat
     7741 ttttcgactt gcgagaagac catgtacgag cgttatgcag gtttaatttt tgatatggat
     7801 ggcacaatcc tggatacgga gcctacgcac cgtaaagcgt ggcgcgaagt attagggcac
     7861 tacggtcttc agtacgatgt tcaggcgatg attgcgctta atggctcgcc cacctggcgt
     7921 attgctcagg cgattattga gctgaatcag gccgatctcg acccgcatgc gttagcgcgt
     7981 gaaaaaacag aagcagtaag aagtatgctg ctggatagtg tcgagccgct tcctcttgtt
     8041 gaagtggtga aaagctggca tggtcgtcgc ccaatggctg taggaacggg gagtgaaagc
     8101 gccatcgctg aggcattgct ggcacacctg ggattacgcc gttattttga cgccgtcgtc
     8161 gctgccgatc acgtcaaaca ccataaaccc gcgccagaca catttttgtt gtgcgcgcag
     8221 cgtatgggcg tgcaaccgac gcagtgtgtg gtcttcgaag atgccgattt cggtattcag
     8281 gcggcccgtg cagcaggcat ggacgccgtg gatgttcgct tgctgtgagt gaagcgttat
     8341 cgcttttctc gttgtttgcc agtagttttc tcagcgctac actattaccc ggcaactccg
     8401 aagtcgtgct ggtggcaatg ttgctttccg ggatcagtca tccctgggtt ttagttttaa
     8461 cagcaacaat gggtaatagc cttggagggt taactaacgt tatccttggg cgtttctttc
     8521 cattgcgtaa aacatcgcgc tggcaagaga aagctaccgg ctggctgaaa cgctatggtg
     8581 cagtcacgct attattaagc tggatgcccg tggttggcga tttactgtgt ctgttagcgg
     8641 gatggatgcg catctcgtgg ggaccggtaa tctttttttt gtgccttggt aaagcgttac
     8701 gctatgttgc ggttgcagca gctaccgttc agggcatgat gtggtggcac taattgtagg
     8761 cctgcacata tggtcaccat tacagttatg ctaattaaaa cgattttgac aggcgggagg
     8821 tcaatttgat cccggacgta tcacaggcgc tggcctggct ggaaaaacat cctcaggcgt
     8881 taaaggggat acagcgtggg ctggagcgcg aaactttgcg tgttaatgct gatggcacac
     8941 tggcaacaac aggtcatcct gaagcattag gttctgcact gacccataaa tggattacta
     9001 ccgattttgc ggaagcattg ctggaattta ttacaccagt ggatggtgat attgaacata
     9061 tgctgacctt tatgcgcgat ctgcatcgct atacggcgcg caatatgggc gatgagcgga
     9121 tgtggccgtt aagtatgcca tgctacatcg cagaaggtca ggacatcgaa ctggcacagt
     9181 acggcacttc taacaccggt cgctttaaaa cgctgtaccg tgaagggctg aaaaatcgct
     9241 acggcgcgct gatgcaaacc atttccggcg tgcactataa tttctctttg ccaatggcat
     9301 tctggcaagc gaagtgcggt gatatctcgg gcgctgatgc caaagagaaa atttctgcgg
     9361 gctatttccg cgttatccgc aattactatc gtttcggttg ggtcattcct tatctgtttg
     9421 gtgcgtctcc agcgatttgt tcttctttct tgcaaggaaa accaacgtcg ctgccgtttg
     9481 agaaaaccga gtgcggtatg tattacctgc cgtatgcgac ctctcttcgt ttgagcgatc
     9541 tcggctatac caataaatcg caaagcaatc ttggtatcac cttcaatgat ctctacgagt
     9601 acgtggcggg ccttaaacag gcaatcaaaa cgccatcgga agagtacgcg aagattggta
     9661 ttgagaaaga cggtaagcgg ctgcaaatca acagcaatgt gctgcagatt gaaaacgaac
     9721 tgtacgcgcc gattcgtcca aaacgcgtta cccgcagtgg tgagtcgcct tctgatgcgc
     9781 tgttacgtgg cggcattgaa tatattgaag tccgctcgct ggatatcaac ccgttctcgc
     9841 cgattggtgt agatgaacaa caggtgcgat tcctcgacct gtttatggtc tggtgtgcgc
     9901 tggctgacgc accggaaatg agcagtagcg aacttgcctg tacacgcgtt aactggaacc
     9961 gggtgatcct cgaaggtcgc aaaccgggcc tgacgctggg tatcggctgc gaaactgcgc
    10021 agttcccgtt accgcaggtg ggtaaagatc tgttccgcga tctgaaacgc gtcgcgcaaa
    10081 cgctggatag cattaacggc ggcgaagcgt atcagaaagt gtgtgatgaa ctggtcgcct
    10141 gcttcgataa tcccgatctg actttctctg cccgtatctt aaggtctatg attgatactg
    10201 gtattggcgg aacaggcaaa gcgtttgcag aagcctaccg taatctgctg cgtgaagagc
    10261 cgttggaaat tctgcgcgaa gaggatttta tagccgagcg cgaggcgtct gaacgccgtc
    10321 agcaggaaat ggaagccgct gataccgaac cgtttgcggt gtggctggaa aagcacgcct
    10381 gacagaaaag aaaaaggcca ctcgtgagtg gccaaaattt catctctgaa ttcagggatg
    10441 atgataacaa atgcgcgtct ttcatatact cagactcgcc tgggaagaaa gagttcagaa
    10501 aattttaaaa aaaattaccg gaggtggcta aatgccgttg ttagatagct tcacagtcga
    10561 tcatacccgg atggaagcgc ctgcagttcg ggtggcgaaa acaatgaaca ccccgcatgg
    10621 cgacgcaatc accgtgttcg atctgcgctt ctgcgtgccg aacaaagaag tgatgccaga
    10681 aagagggatc cataccctgg agcacctgtt tgctggtttt atgcgtaacc atcttaacgg
    10741 taatggtgta gagattatcg atatctcgcc aatgggctgc cgcaccggtt tttatatgag
    10801 tctgattggt acgccagatg agcagcgtgt tgctgatgcc tggaaagcgg caatggaaga
    10861 cgtgctgaaa gtgcaggatc agaatcagat cccggaactg aacgtctacc agtgtggcac
    10921 ttaccagatg cactcgttgc aggaagcgca ggatattgcg cgtagcattc tggaacgtga
    10981 cgtgcgcatc aacagcaacg aagaactggc gctgccgaaa gagaagttgc aggaactgca
    11041 catttagtca gtttaagcag aagaggccgg gctggtgaaa acaccgcccg gcttttgtta
    11101 cctgtcagtc atccgatatt ccacgctatg tgcttcatca ggttgaggaa cttccagcct
    11161 tgaggccatc cgcatcacga ccgcttcagg aactgcatac tcgcgtctgg cattttgctg
    11221 cttccattgc gcccatggca cttccagata aacgattttt actcgtgcgc cgtaagccgt
    11281 aaacagactg atgagctggc tgcgcagctg gcgggtaatg ttcgttgcat tccagatgaa
    11341 cggctttttc tggcgtaaaa aaacgcgcgc ctcttctttt gcctgttgca caatacgccc
    11401 ggtggcggtt ttgtcgtccg gactggcatt gattcgccgc cgcatatcat caaggctgat
    11461 aacgtccatt ccctggcact gttcactgat atagcgatct ttgcccatac ccggtaagcc
    11521 acacagcaaa ataacctcaa aaggttcagc ctcccaggga acaaaatcag gagaactttg
    11581 ctcatgagtc aggtagtgcc agcgtgcgga atcagaaacg aacgggcgca tttttcccca
    11641 gcattgttgc tcatggcaaa acagctcgaa cagatcgatg cgttccagca ttgattgctg
    11701 atccgggctt tggcgaccaa gcagatcggc acgagcaagc aaagcgagca gacgcgtgtc
    11761 gatgcgcatt gcagccgtga gcagcaaacg ctccggttcc ggacgttcca gtaaccatag
    11821 cggtaaccca tgcagacgca ccagcgcaac aatttgctcg cgtagcacga acggcgttgg
    11881 gatatcgcgc cataaaattt gtcgggccgt cagttcgccc cgacgagcat ggccaggcga
    11941 ctgaatacgt ccgttttcct gcacggtagt gctgcgcttt tcaacatcat gcaataacgc
    12001 cgccgcccat aaaacttcct gctgttgagc ggggagttgc tggaattctg gctgagtaac
    12061 gagggcgttc agcaccattt cggtatgcac gccgacgtca ccttcaccat gatgctccgg
    12121 gtcttgtggc gtgtgatgca tctcctcaac ccagctaaaa cgctgacgta gcgccgacca
    12181 gcgtttgtcg tcggtaagct gccaaatcat gactgttctc cataatcgaa ctcatgggcc
    12241 atccgcgccc gttgccagtg tcgtttccag tggacggtgg ttttgacatg atttttgcgc
    12301 acatacttga agacgttatg cgaaaagtca gcgacggaaa acgcatcacg attcctgctg
    12361 acaatgcctt ccagcgtaca gggttgcccg gtttgcgtat cccagggatc gaacgcgccg
    12421 cgcgcattgg tcagcgccag aaaatcacgt tgccagcttt tttcatcatt ccccggttgc
    12481 gggccggaga tttcgggaac gcaggggaaa tcaaacagag cggcgtaaaa ctgcacctct
    12541 tcccagctca gccacatatc ctggcagcga acggcaaaca gataaaaatc ctgctccagg
    12601 gcgcgatatt caatcgaatg cacggcataa agattttcac caaacaactc cagatcgccg
    12661 agatcgtttt tcagcagttg ccagcgttgg cgaattttat aggtccatgc cgactgcgta
    12721 ggcgcggcat gggaacgggc aaacacgccg tagcggttaa ggcagttatt ttcaccgtcg
    12781 agtttttctg tgtgcaccaa ttgcgtgatg gcctgtaaat cctgccagta atcggcatta
    12841 atgcggtcat cgctggtggt gccgggggag aaaggataat gccaggttct cccgtatttt
    12901 cgttgggtat tcataataat ccattatttt gtgtgtaata aatgtacgcc cgcgagggtg
    12961 cgagttatgg ggttaaaaga aaaatgtgag tatcacccgc gaaagcgagg gcgaaagaaa
    13021 aagagaatta acaacctgag cgaatgctca gaaccggtta atggtgaatg gaagagtttg
    13081 gggcggcgaa aagatacacc gaatgagtgg ataaaacaac cgtttgcaaa atgagcaaat
    13141 aaaaagggag ccgcagctcc ctttgacaac cttagtgcgc accgcctccg ccgccacctg
    13201 caccaaatgg cggtttagca aaccacacca gccccagcag gacgaggaat attccggctg
    13261 acatccagaa gatctcattg gcggaaataa tcagcccctg attagtgatc tgctgggcaa
    13321 tccagcctga tgcctgttgt tgcgtcatcc ccagcccttc cagttggctg tacatcgcct
    13381 gagcgttcgg gttaaacggg tttaccgact cggttaactg tgcgtggtgc atcgactcgc
    13441 ggttagtcca catggtggtg gtgatggatg tgccgataga ccccgccagc gttcgcgtaa
    13501 agttggagag gctcgatgcc gccgccagtc gttccggtgg caaaccggac agcgtaatgg
    13561 tggtcagcgg cataaagaag caggccaccg caaacccctg gataaactgc ggccaggccg
    13621 acgcgccaaa atccatacct ggttcaaagg tataggcgcg ccagtagaag cagacggcat
    13681 acataataaa gctgaaggtt accagccgcc gcatatccag tttatgcgcg aagcggccga
    13741 taatcggcga caggatcacc ggaataatcc ctaccggcgc agaggccaga cctgcccacg
    13801 ttgccgtgta accgtagacc tcctgcaaca actgcggcag cagaacaatc gcgccgaagt
    13861 agagcatata cgcgaggctg atacacaagc agccgatggt gaagttgcgc gacttaaaca
    13921 acgacagatc gactatcggg ttatcgtcgg tcagctccca gacaatcagg aagcagatag
    13981 ccaccactgc caccacggta aggatgataa tttcctgtga tgagaaccag tccagctctt
    14041 taccgcggtc gagcataatc tgcaggctac cgataccaat aaccagcagt gccagcccca
    14101 cggcatcaat ccgccgccgt tcggtgcggg tttcacgtcc gcgcagggtt tgcagtgtca
    14161 tcaacaccac cgccacgcca atcggtacgt tgatgaagaa tatccagccc cagtggtaat
    14221 tgtcactgat atacccgccg aggatcgggc cgcaaattgg cgcgacaatc accgtcatcg
    14281 accacaacgc cagcgcgatc gagcgtttgg ctggcgggta gttattaagc aatagacttt
    14341 gcgaaagcgg gatcagcggc ccggcgacaa tcccctgaat cacgcggaag aagatcagca
    14401 tattcaggct gctggagaca ccacacgccc acgacgcaat agcaaaggcg atggtggacc
    14461 agaggaacag tttcacttcc ccgacgcgct ttgccagcca gccggtaagc gggatcgaga
    14521 tggcattcgc caccccgaaa gaagtgatta cccacgttcc ctggctgagc gatgagccca
    14581 gattcccggc gatagtgggg atcgccacgt tagcaatggt ggagtccagc acctgcatga
    14641 atgtcgccag tgacagcgca atcgtcataa tgaccagttg cgcgccttcc agcggttttt
    14701 gctgttgcat cacacgcacc tctggattag ccagcgttag cttttacgat atcgtcgatc
    14761 agtttattga caggtgccag gctgatttca cgcgcggtgc ttaccgctac cggtgtggaa
    14821 cgtactttat ttgccagtac ctgaccgtca cggttagtgg tattgacgct caccagcgtg
    14881 gacaaaccga tacgcagcgg gtattgctcg agctgtttct ggtccagttc aatacgaaca
    14941 ggcagacgct gaacgacttt gatccagtta ccggttgcat tttgcgccgg aagcagtgag
    15001 aacgcgctac ctgtgcccat atccagacca accacttttc cggtgtattt cacatcatcg
    15061 ccgtaaatat cggtggtgat ggtgaccggc tgaccgatac gcatattggc aatctgcgtc
    15121 tctttaaagt tggcatccac ccacatattg gtggctggaa cgaccgccat cagcggcgtc
    15181 gttgggctaa tttgcgcccc aggctgtacc gcgcggcggg agacataacc ggtcatcgga
    15241 ctgacaatac gggtacgctc cagcgccagc caggcgttac gtacttcggt ggcagcctgt
    15301 tgcacggcag gctgatcttc cagtttagtc cccagaatca tcgcctgatt ggcattgtat
    15361 tgttgaatag cgacgtccag ttgtgcctgg gcactggtaa cggcgtcgcg ggcgtgttgc
    15421 agctcttcgc gaccaatcag gttggcattg cccagcggca cacggcggtt gtagtcgctt
    15481 tgtgctttcg cgagggcgat tttttgcacc tcaatattcg cctgcaactg tttgctgtta
    15541 atcatcagct ggtgggtttg gcgaacgctg gaagccagtg cagttttggc tttttcaaac
    15601 gcctggcgag catctgtcgg gtcgagagtg accagcacat cgccttcttt tacaaaatcg
    15661 gtgttatcgg cccagacttt cgtcacgctg ccagacacct gagacataat ttgaatctga
    15721 ttccctgcca cgtatgcgtc atcagtttct tcgaaatgac gcagtaccaa aaaccaataa
    15781 atccctatcg ctacggcaat aattataaag agcaaggtga gaaggaggag cagacgctta
    15841 cgtttgccgc tcttctttac cggttgctgc ggggtttgag tctccgcatt tgcgctcata
    15901 ttgttctcca cgatcttctt aactcatcgg ctgagccgac ctgcttgcca aaaaggccag
    15961 catgttgcga tgctggccag tcattttttc ttttataaat ctggattttt gagcgagatg
    16021 acgcgttagc tcatcgcttc gagaaccaca ccgtcttgtt ccatctggtc gagacgggag
    16081 agcaatttgc gggtgatttg ctcgagctga tctttttctg ttgtgctgag cgcggaccag
    16141 agttgatgca ggcagttatg ctgcggtggt aaaacctcgc gcaaaaactc gtgacctttt
    16201 tccgttaatt gcagatgcag gcagcggcga tcgttatcgc tttcacgacg ttcgatccaa
    16261 ccgcgttttt ccagttcatc ggcaatacgc gtcgcgttgg tacgggatga tccaagagca
    16321 caactcaatt cagaaggctg aatactgtgg ttttcctgag actccagcgt aatcaacgcc
    16381 ataaacaacg tctcgttaat cccctgagcc ttcagcattt tattgcggtt ctccagcagc
    16441 ttgctttgca tgtgcatgca aagacgggtc agaaggatct cctgataagg aaaatcttcg
    16501 tggcggctgg cgcgaaattt tagcatttgt tcaatgggcg taaacgaact atccatttgg
    16561 gtatgacctc attaatttca gccgatatag taacgacagt gacaaataaa gtaaatgtat
    16621 tgttttagaa aaatgattct tgtgggttat ataatcgcca tcactttcca ggcgagccca
    16681 taggccagcg cactaagcag tgttgggatg ataatgctgc gtgttttata gaaactggca
    16741 cccagtaccg cgaagccgac cagagtgggc acgaaacggc gtgtatcgtg catcacttct
    16801 ggtgcggtag agacaaccag cagagcgcat atcgaggcga tgccaatggt gtcgagcaaa
    16861 atacctaccg cgccacgttt ggttgggcgg gcattaccca cacgcaggcg cagcggcaaa
    16921 tagcggaagc aataattcgc cgcgccaacc agtaacccaa gcagcagaac ctcatagctc
    16981 atcgggcgct ccttgccaga atgcctggat taacgcagtg aggcagccac agacaatgcc
    17041 tgccagaatg gcgacgggaa tagaaaatag cgttacgcct gcaagggcac caactaacgc
    17101 tgcggtaacg caaagagatt gtttgcgctg gaaagaggcg agcaggaaac tcataaagag
    17161 tgccggaagc ataaaaccta atgctgcttc aacggcggga tatccttgca gcaagccgct
    17221 gccggagaat gcccctatta ccgtaccaaa tacccacgat gaccatgaac tgaaggcaat
    17281 gccgatcatc cagttctcgc tccagcggcg attattgcgt accagttttg cggttgcggc
    17341 ggcaaaaacc tcatccgtca ggccaaacgc ccacagggca gttttcgatt tttgcagacg
    17401 ctgaataata cggctacgca gtgacgggcc atacaaaaca tggcgaacat ccattgccat
    17461 gacggtcagt gcagcaaccc acaaactact cccggctgcc agcatcgcgg taatgacgaa
    17521 ctggctcgcg cctgcataaa tgatgcagga gaaaaaaacg ctttcgagag gagagaatcc
    17581 cagacgggtc gcattcagac cgaacgcaaa ggccaccgga atataactaa taacaatcgg
    17641 taaactgtct ttgcatcctt ccatgaaggt cgccgaacca ggagcaggct gtggagtagg
    17701 gctttccata gagtgtacgc ttaacgattg ttaatattat aaatagactg aatgaatatc
    17761 ttaaccttat cagactgatg ggcttcttaa cacccttata agtgtaaagc cacgaaaacg
    17821 gttgctgatt gcttaaattg cggcttcctg acgatgaaaa cctcgccacc agaccagtaa
    17881 gttaaccagg gcaatcgtcg cgccagccag acaaacgcca gcccaaccgc catgttgcca
    17941 gactgaggct gaaattagcg aaccggcggc accgccaatg aagtagctgg tcatgtaacc
    18001 tgcggtcagg cgattacgcg catcagggtg tattcgataa attaccgtct ggttagtgat
    18061 atgcacgccc tgcacggtga gatccagcac caggatgccg ataatcagcg ccagtacgga
    18121 agtgtgacca aaccagatcg ccagccatgg aagtaacagc agcagcagac cgaaagttgt
    18181 ggtgtggtgc gatttgccct tatcggcaaa tccgcccgcc ggacgagcgc ccaacgctcc
    18241 ggctgctccc gcaagtccaa acagaccaat gacgccatcg ctgtaattaa aaggcggagc
    18301 ggcgagcaaa aaggccatcg aggtccagag aatgctgaag ttggcaaagg tcaggcagcc
    18361 cagcaacgtg cgggtgcgca gaattttatc gctgataaac atgctgaaaa cggaaccaag
    18421 caactgtggg tagttgaggt gggtttctga tttcatttgt ggcagaccac gccataatgc
    18481 cagcgccatc agtgccatta acaccgaagc aacccaaaag acggtgcgcc agccgccgag
    18541 gtttgccagc aacccggcaa cggttcgtgc cagcaagatc cccaacagca gcccgctcat
    18601 aatggtgccg accactttgc cgcgtttgtc cggtgaagcc agcgtcgctg ccagcggaac
    18661 cagaatttgt gccacgaccg agaataaacc ggttaatgcc gtaccgagga tcatcatcgc
    18721 cagcgactga ctgctggcag tgattaacat gccgcatgcc gccagtaagg tcatcgagac
    18781 aatcaggcgg cggcgttcaa acatatcacc gaggggaaca agaaacagca gacctgcggc
    18841 atagcccaac tgcgcggcgg taacaataaa gcctgccgaa ctggcggaaa gggaaaagtt
    18901 acgcgcgatg gtgtcgagca atggctgggc gtaatagtta ctggctaccg ccaggccggt
    18961 ggcgatagac atcagcacga tcagcgccgg gctaagctca tgattaggtt tagtcattat
    19021 ttttgttcat caggtgatga ttaagctgga aatcaataat agcggatgtt gatgaatatg
    19081 ggatgaaaga tagattgttg aaatgtgcgg tgtggtttgt aggtatgata agacgcacca
    19141 gcgtcgcatc aggcattgtg tacagagcgg cggatgaggc aaaaacgcct tatccgcccg
    19201 aataaaaatt acttctgcgc tgccagcgcc tcattcaccc agccatcgaa ctgctgctgg
    19261 tgggctttga tccagccatc aacgtggccc tgaatatcgc cttctgaggc tttgccgtca
    19321 tgcataatgg cgttctgggc gttaatatca gccactggta actgcataat ggcaaacagt
    19381 ttcgctgctg ccgggttttt ctcggcccag gctttgttgg caacgatatg catggtactg
    19441 accgggaagc cgtaattcgc gccattcggc agtttggtat cggcattttt atcgcccggc
    19501 agtgcggaga acggcacctg caaccagacc acatctttcc ccggtttcag ttcgttactc
    19561 acccagtacg gcgtccaggt gtagtaaaac accggcttac cctctttgta acgactgatg
    19621 gtgtcagcca tcatcgctgc gtagttcccc tgattatgcg tcacggtatt ggtcagtcca
    19681 tacgcggcaa gctggtggtt gatcgcacct tcgcagcccc agcctgggtt acagccggtt
    19741 aaatccgctt taccgtcgcc gttggtatcg aacagtttgg cgatcttcgg atctttaagt
    19801 tgcgcgatgt tggtaatttt gtactggtcg gcagttttct tatcgatcag gtaaccctgt
    19861 gccgcgccgt taacaaatac tccttcacga taaaatttct tatcgccacc ggcagcttcg
    19921 tacatgttgt catgcagtgg cgtccagttc actgcggtga aggttgcatc gccggaagca
    19981 agcgaggtgt agccaacgtt gtaatccact tcgctgggtt tattgacggt ataacctaat
    20041 ttctccagcg cgcggctgac cagcagcgtc tggaaagttt cttcggtgat ggtgctctga
    20101 accggattaa cagtaatgcc tttgcccggc aggtcggcag caaaagtttg tgtagagata
    20161 agcgtggcaa acgctgtcgc aaaaagtacg ctatgtcgca ttgttattcc tttttttggc
    20221 agggtgataa tgcccggcgt caccgggcaa gtgcagagtt acttaatgaa tgggcgggtc
    20281 agcagaccga cagggccagt ggtgtaccag cgacggttgc cgcgactgcg tgagtcgcgc
    20341 ccaacggcct gcgtcaggcg gtcgaggata atggcgagga tcacaatccc gacgccgcca
    20401 acggtggcaa gccccatatc cagacgaccg ataccgcgaa gcaccatctg acccagcccg
    20461 ccgacggcaa tcatcgaggc gatgaccacc atagaaaggg ccagcatcag cgtctggtta
    20521 acgcccgcca taatggtcgg catcgccagc ggtaactgaa ctttgaacag catctggcgc
    20581 gggctggcac cgaatgagcg cgaggcttca atcagatccg ccggaacctg gttaattccc
    20641 agaatcgtca gacggataat cggcggcagc gcaaagatga tcgtcaccac cacgcccggc
    20701 acgttaccga taccaaacag catgacgatt ggtaccagat aaacaaacgc gggcgtggtc
    20761 tgcatggcat caagcagtgg acgaataatt ttcgccgctc gcggacttct cgccagccag
    20821 atccccaacg gcaaaccgat gacgatacag aacagcaggg cggttagcac cagcgccaga
    20881 gtaaccattg cctgtgacca ggcaccgatt gcgccgatgg caatcagcga aaccagtgtc
    20941 gccacgccca ttccgacacc ggaaatttgc caggcgatga gagcgaaaac gataatcgcc
    21001 accggagcgg gcatacccag cagcaattgc tggaaaccgt tgaggatgta atcaaccgga
    21061 acgcgcacgc cctgaaagac gggacgaaaa tgggtaacga cccagtcgat cccttcagtg
    21121 acccaactgt cgagcgggat cagcgtttta tggaacggat cgagaatatt aaaatgctcg
    21181 acgtttggcg caggcgtact ggtcagccag tcagcaccac cgccgtcagt cggtgcagca
    21241 gccggtgtac cccaggcgtc tgcggactgt gcggcactgt ccgccgctgg cgtggtatcc
    21301 cacggattat tttgatcagc cattatttac cccctcacga tctaaagcgc gcagcagcat
    21361 tcctttcgaa atgatgccga catactgttg gtcctcatcg accacgggca ccgcacaggg
    21421 ggcctgtccg acatgagaga gcaactcgct aagaggcgtt tgtgcatcga ccgctaacgg
    21481 cgcatcaatc agcgccgcat caagaccttg ctgctgcgtt aacgcggctt taagcgaatc
    21541 gatagacaca gcgccgacaa acttattacc gcgttcgatg acgtagccat attcgcgatc
    21601 ttcatcctgc aataatttca gtgccgaacg cgggccgaag ccgggggttt tacgaattaa
    21661 gccgttcggt gtacgacggg caatatcttt cgcactgaac acctggctaa tatcgacgcc
    21721 acggaagaag gtacggacat aatcattcgc cggattattg agaatttcat ccggtgtgcc
    21781 gacctgtact acttcaccat tttgcataat ggcaattcgg tcgccaatac gcatggcttc
    21841 atcgagatcg tgggaaataa agacaatggt gcgctgatgt ttcgcctgta attttaccag
    21901 ctcatcctgc atctcggtgc gaattaatgg atcgagcgcc gagaaggctt cgtccattaa
    21961 taatatatcc ggattaatcg ctaacgcgcg ggctaatccc acacgttgac gcatcccgcc
    22021 agagagttca tccgggtagc tgtgggcata attttccagc ccgacctgac gcagtgcatc
    22081 aagggctttt tcacggcgtt cttcggcatt aattccggcc agttccatac cgaacgcagt
    22141 attgtccagc acggtcatat gcggcattaa ggcaaaggac tggaagacca tcgcaatctt
    22201 ttttctgcgc acctcacgga gttcggcgtc ggatattttg gcaatatcca caccatcaat
    22261 cagcacttgc ccgcgggtgg gttcaatcag gcgattgaga aggcgtacca ttgtggattt
    22321 acccgaaccg gataatccca tgatgacaaa tatctcgcct tcttcaatgg ccagactggc
    22381 gtctttaacg ccaagcgata gcccagtttt ttccagaatt tgttcttttg aaagtccttg
    22441 ttcgatatat ttgaaagctc gctgtggatg ctcgccaaat attttataaa gatttttaat
    22501 ttctaattta attgccatgc aatagaaaga ttcctgttat ttgtttatgt cgatacatta
    22561 cccaatggaa atagtcactt ttttctaccc taacatactg agaatctgag gcaacccctg
    22621 atggcaaatg tggcatgaat attgatgtga gcaaatcgcc ggaatttccc atgatataag
    22681 ggctgagagc aaatcgagaa aagttgatat tttttgtcaa agatagcaaa aaaacgcctg
    22741 aatattgtgg tttcaggcga ataatgcaac agagtatcag tgtagatcac cacaaattat
    22801 ttgcgtgata taaagctatt tggcgggaat tatttccctg cagcgggtag tgatattttt
    22861 gaaaataacc ccctcagaaa ttccagtctt catcttctgt ttcgaccgct tttcccatca
    22921 catatgaaga gcctgaaccg gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg
    22981 cggcgaggat tgccggattc acttccgcca tttctgcggg aaatagcggc tcgtagccca
    23041 gattcatcaa cgccttattg gcgttgtaac agagaaacgc tttcacatcg tcagcccacg
    23101 gggtttcggc gtacagctca tcggtgtatt gcaactcgtt gtcatagagt tccagcaaca
    23161 aatcgaaggc gaaactcttc aattcttcac gtcgtgcctg ggagatcttt tccaggtttt
    23221 tctgatattt atagccgatg tagtaaccgt ggactgcttc atcgcggata atcagacgga
    23281 tcaggtctgc ggtattggtt agctttccgc ggctggaaaa atacatcggc agccagaaac
    23341 cggaatagaa caaaaaagat tcaagaaaca cactggcgat tttctttttc agcggatcat
    23401 caccgcgata atgttgctga ataatctgtg cttttcgctg caacggcgcg ttttcttcac
    23461 tccaggcgta ggcggcatcg acatctttgg tctggcacag cgtcgagaaa atcgaactgt
    23521 aagagcgggc atgaaccgcc tccataaagc tgatattcga taatactgcc tcttcatgag
    23581 gcgtgagcgc atcgggcatc agcgaaggcg cgccgataac attttgcagc gtgtcgagca
    23641 gtgtcaggcc agtaaaaacg cgcatcgtca gttgttgttc tacgacactt aatgtctgcc
    23701 aggcgggaat atcgttcgat agtggcacct tttctggtag ccagaaattg ctggtcaggc
    23761 ggttccacac ctccagatct ttatcgtctg ttatcttgtt ccagttgatg gcgctgatac
    23821 gtgagagttt catagatatt ccttaaagtg cacaggagac gcagccttca atttcagtgc
    23881 cttccagcgc catctgacgc aggcggatgt agtagagcgt tttgatacct ttgcgccagg
    23941 cgtaaatctg cgctttgttg atatcgcgag ttgtggcggt atcggggaaa aacagtgtca
    24001 gcgacagccc ctgatcgaca tggcgagtcg cttcggcgta ggtgtcgatg atcttttctg
    24061 cgcctatttc gtaagcgtcc tgatacagcg ccagattctc gttagtcata aacggggcag
    24121 gatagtaaac gcgtcctgtt ttgccctctt tgcgtatctc tactttcgcc acaatcggat
    24181 gaatactcga cgtggcatgg ttgatataag agatagaacc ggttggcggc accgcctgga
    24241 gattttggtt gtatatgcca tagcgcatca cgtcgtcgcg cagttgcgcc cacatttcgc
    24301 gggtaggtaa cgtgatgctg ctacgagcaa acaactcgcc aactttcgcc gttttcggtt
    24361 gccagttact ttgcagatat tggctaaaat attcaccgct ggcatagcgc gactgtttga
    24421 acccggcaaa ggtttcgccg cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc
    24481 aggtgatggt atagaaatag agattggtga aatcgagtgc ttccggcgat ccataagcga
    24541 tgccttctcg cgccagataa ccgtgtaaat tcatctgccc cagtccgatg gcgtgtgagg
    24601 cggcatttcc ggcttcgatg gacggcacgc tgcggatatg actcatatct gacaccgccg
    24661 ttaaaccgcg cacggcagtc tctaccgtgc gggcaaagtc gggggaatcc atggtgtggg
    24721 caatattcaa cgaacctaaa ttgcaggaaa tatcatggcc tgtgcgggca tagtcgagat
    24781 tctcgtcata ctctgaggcg ctgttaacct gcaaaatttc tgagcagaga ttgctcatat
    24841 ttatgcgccc ggcgataggg ttagcacggt taaccgtgtc ttcatacatg atgtacggat
    24901 agccggactc aaactggatt tctgccagtc gctggaagaa atcacgggcg ttgatgtatt
    24961 ttttgcggat gcgttcattg gcaaccagtt cggcatagtg ttcactgatg gcgacatcgg
    25021 caaatggctt gccataaact cgctctatgt catagggcga aaacagcgcc atctgcgcat
    25081 tctcttttgc cagatggaaa gtgatatccg ggatcaccac gcccagcgac agtgttttaa
    25141 tgcggatttt ttcgtcggca ttttcccgtt ttgtatcgag aaaacgcaga atatcgggat
    25201 gatgagcatg taaatagact gcgccagcac cctgacgcgc gccgagctgg ttggcatagg
    25261 aaaatgcgtc ttccagcatt ttcatcaccg gaatcacgcc agaagattga ttttcaatac
    25321 gtttaattgg cgcgcccgct tcccgcagat tcgacagcaa aaatgcaacg ccgccgccgc
    25381 gtttcgacag ttgcagtgcg gaatttactg cccggccaat cgactccata ttgtcttcaa
    25441 tacgcagcaa aaaacaggaa accagttcgc cgcgctgctg cttaccacag ttgaggaatg
    25501 tcggtgttgc aggctgaaag cgtcctgaca gcatctcatc ggtcagttgc gtcgccagtg
    25561 tttcatcgcc ttgtgccagc gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca
    25621 gataacgctt accgtcgaat gtcttcaacg tatagctggt gtaaaacttc catgctccga
    25681 ggaatgtctg aaaacgaaaa ccgctggcgt gtgcgtgggc aaacagcgta atgacaaaat
    25741 cgctagagta gcgattaagg acactttcat catagtaacc ttcgttaacc agccagttca
    25801 ggcgctgctg ctggctactg aaggccacac tgttcggacg cacatgcgtc gtcataaagg
    25861 cgtctactgc ctggcgatct ttatcgaact gaatgcgacc tgcgctatcg taaaggttaa
    25921 gcatcgcatt cagcgcgtgg taatccatag tttcctgcgt caggcgttct gcggttgtcg
    25981 ttgccaaaat tcggttactc ctttacgaac gttttcgata tcgctttgcg tacccatgag
    26041 ttcaaagcgg tacagccatg gcacgccgca tttccgggca atcacatctc cggcgcggcc
    26101 atatgcctca ccaaagttgc gattacccga agcaataacg ccgcgcagca acgcccggtt
    26161 atgctcgtcg tttaaaaagc gaatgacctg tcgtggcacc gcgccagccg taccgccgcc
    26221 gccgtaagag ggcacgatca ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt
    26281 gagcgggatg cgcaccgcgg gcagccctaa acgttcgata aaacgctgcg tattttcgga
    26341 gctgctggaa aagtagacaa tctggctcat gcattggccg cgtgtggtgc tggatgcaga
    26401 cggttgatca tgtccgggcg gaaacctgac cagctaagat cgcctgcaat cacgaccggc
    26461 aactgacgaa agccctgagc acgcagcgct tccgccgctt caggaacgcg atcgacatta
    26521 atcatttcaa agtcaaagcc ccggttttcc atcgcccttt tggtggcgtg gcactgaacg
    26581 caatcgttac gagtgtaaat agtaatgcgc atgattcgta tttccgttta aaatgaagat
    26641 acggcgcgat gatacgcgtc gggttgtctc tctgttgata cagaaatact agatgtagtt
    26701 gaaaaaagat tcaaccacac aatatatagc aaatagtggt cgaaattacc ctgggtatga
    26761 gcgtgacggg gtagggggat ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg
    26821 cttcggggtt aaagacgcgt atttattatc atgttgcact acgatttgcg catgcttaac
    26881 agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca gggtctttca
    26941 cgaacaaaag aatccgcgca gccaacggca tcgcgcgcgg cttgctggac gcgagtacga
    27001 ccatgcattc gcgctctggt ttctttcagt agagcctgcg ctttactacg agcggcttcg
    27061 gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgagact atcagctaat
    27121 tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg gttcggtcgg
    27181 ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc ataattttct
    27241 taactgaggt tcatcgcagc tttatggact tttctgaaag cgttgtgaat gcgcaatcag
    27301 gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg accagacgag
    27361 gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca gaaggcttat
    27421 ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat gggacgtacc
    27481 gccctggtga ttcatccaac attaaaagaa cgtagttcaa cactggctga acccgcttcc
    27541 gatattaaaa cttgcgatca ttatcaacaa tttccgctct atttagcagg cgagcgacac
    27601 gagcattacg gtatcccgca tggctttagt tcgcgtgttg cgcttgaacg ttatttgaat
    27661 ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg aaaagaaggg
    27721 tcaggctttt acctgctggt aacggctgac tttaaacagt cggcggcaat aatcgaggaa
    27781 gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga ccgccgccat
    27841 aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg gtgactggaa
    27901 cgtcacataa gccatgatat ccgccagatt ttttatccag cccgacgggc taactttgcg
    27961 tgctgcgcgc ataaacagat cgcggtacat accgtatggc catgcaatta agatgtttac
    28021 cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg agaggaaaac
    28081 ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat ctgcaactgc
    28141 atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa gcgtaatggg
    28201 atgtgaatgg agatgaaagc agtggatctc attgccatct gcatttttgc atagcgtata
    28261 tcgctggcta tgactaatca attagtgaaa aatttttatt taatctgttt ttgtagataa
    28321 aatattccgt agcggcgcgt tttttgtaca tt
//
LOCUS       DAZGAP010000049        28206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGAP010000049 DAZGAP010000000
VERSION     DAZGAP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.049"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            259..930
                     /locus_tag="ACHLAM_001770"
     CDS             259..930
                     /locus_tag="ACHLAM_001770"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO7853306.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDIEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            940..2136
                     /gene="yaiP"
                     /locus_tag="ACHLAM_001771"
     CDS             940..2136
                     /gene="yaiP"
                     /locus_tag="ACHLAM_001771"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO7853307.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2171..2695
                     /locus_tag="ACHLAM_001772"
     CDS             2171..2695
                     /locus_tag="ACHLAM_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7853308.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2697..3470
                     /gene="yaiO"
                     /locus_tag="ACHLAM_001773"
     CDS             2697..3470
                     /gene="yaiO"
                     /locus_tag="ACHLAM_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO7853309.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3655..3930
                     /gene="frmR"
                     /locus_tag="ACHLAM_001774"
     CDS             3655..3930
                     /gene="frmR"
                     /locus_tag="ACHLAM_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO7853310.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3965..5074
                     /gene="frmA"
                     /locus_tag="ACHLAM_001775"
     CDS             3965..5074
                     /gene="frmA"
                     /locus_tag="ACHLAM_001775"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO7853311.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5166..5999
                     /gene="fghA"
                     /locus_tag="ACHLAM_001776"
     CDS             5166..5999
                     /gene="fghA"
                     /locus_tag="ACHLAM_001776"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7853312.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFAPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6226..6765)
                     /gene="yaiL"
                     /locus_tag="ACHLAM_001777"
     CDS             complement(6226..6765)
                     /gene="yaiL"
                     /locus_tag="ACHLAM_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO7853313.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDYLMW"
     gene            complement(6867..8078)
                     /gene="mhpT"
                     /locus_tag="ACHLAM_001778"
     CDS             complement(6867..8078)
                     /gene="mhpT"
                     /locus_tag="ACHLAM_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO7853314.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYFGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGIGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8254..9267)
                     /gene="mhpE"
                     /locus_tag="ACHLAM_001779"
     CDS             complement(8254..9267)
                     /gene="mhpE"
                     /locus_tag="ACHLAM_001779"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO7853315.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9264..10214)
                     /gene="mhpF"
                     /locus_tag="ACHLAM_001780"
     CDS             complement(9264..10214)
                     /gene="mhpF"
                     /locus_tag="ACHLAM_001780"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO7853316.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10211..11020)
                     /gene="mhpD"
                     /locus_tag="ACHLAM_001781"
     CDS             complement(10211..11020)
                     /gene="mhpD"
                     /locus_tag="ACHLAM_001781"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO7853317.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11030..11896)
                     /gene="mhpC"
                     /locus_tag="ACHLAM_001782"
     CDS             complement(11030..11896)
                     /gene="mhpC"
                     /locus_tag="ACHLAM_001782"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO7853318.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11914..12858)
                     /gene="mhpB"
                     /locus_tag="ACHLAM_001783"
     CDS             complement(11914..12858)
                     /gene="mhpB"
                     /locus_tag="ACHLAM_001783"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO7853319.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12860..14524)
                     /gene="mhpA"
                     /locus_tag="ACHLAM_001784"
     CDS             complement(12860..14524)
                     /gene="mhpA"
                     /locus_tag="ACHLAM_001784"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO7853320.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14715..15548
                     /gene="mhpR"
                     /locus_tag="ACHLAM_001785"
     CDS             14715..15548
                     /gene="mhpR"
                     /locus_tag="ACHLAM_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO7853321.1"
                     /translation="MQNNEQTAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15625..16707
                     /gene="lacI"
                     /locus_tag="ACHLAM_001786"
     CDS             15625..16707
                     /gene="lacI"
                     /locus_tag="ACHLAM_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO7853322.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            16830..19904
                     /gene="lacZ"
                     /locus_tag="ACHLAM_001787"
     CDS             16830..19904
                     /gene="lacZ"
                     /locus_tag="ACHLAM_001787"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO7853323.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSKEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDISLLHK
                     PTTQISDFHVTTRFNDDFSRAVLEAEVQVYGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVLRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLTENLSVTLPSAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQTEVALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19956..21209
                     /gene="lacY"
                     /locus_tag="ACHLAM_001788"
     CDS             19956..21209
                     /gene="lacY"
                     /locus_tag="ACHLAM_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO7853324.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21275..21886
                     /gene="lacA"
                     /locus_tag="ACHLAM_001789"
     CDS             21275..21886
                     /gene="lacA"
                     /locus_tag="ACHLAM_001789"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO7853325.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVHFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVESSV"
     gene            complement(21989..23170)
                     /gene="cynX"
                     /locus_tag="ACHLAM_001790"
     CDS             complement(21989..23170)
                     /gene="cynX"
                     /locus_tag="ACHLAM_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO7853326.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23176..23646)
                     /gene="cynS"
                     /locus_tag="ACHLAM_001791"
     CDS             complement(23176..23646)
                     /gene="cynS"
                     /locus_tag="ACHLAM_001791"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO7853327.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23677..24336)
                     /gene="cynT"
                     /locus_tag="ACHLAM_001792"
     CDS             complement(23677..24336)
                     /gene="cynT"
                     /locus_tag="ACHLAM_001792"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO7853328.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24446..25345
                     /gene="cynR"
                     /locus_tag="ACHLAM_001793"
     CDS             24446..25345
                     /gene="cynR"
                     /locus_tag="ACHLAM_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO7853329.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(25347..25438)
                     /locus_tag="ACHLAM_001794"
                     /pseudo
     CDS             complement(25347..25438)
                     /locus_tag="ACHLAM_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25478..26761)
                     /gene="codA"
                     /locus_tag="ACHLAM_001795"
     CDS             complement(25478..26761)
                     /gene="codA"
                     /locus_tag="ACHLAM_001795"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO7853330.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26751..28010)
                     /gene="codB"
                     /locus_tag="ACHLAM_001796"
     CDS             complement(26751..28010)
                     /gene="codB"
                     /locus_tag="ACHLAM_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO7853331.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         6750 a         7667 c         7315 g         6474 t
ORIGIN      
        1 atgcaattaa ttgacaaact aaatcacatt taaatacaat atataaaaca aaataaccat
       61 acaaaccaaa cacacaaaca cttaacatat gaactacata aagattatac agtcttatcc
      121 ccgtacctgg cagccccttg tttttcagct atcagctatt tttaacactt aaaaattagc
      181 atctgtctgc gtcagaggtt acgtctctgc gggcgtcgtt gtgcttgtag tataataaat
      241 aaaatggagt gtcataaagt ggataaggtt ttagattcag cgatcctttc ttcggcaaat
      301 aaaagaaagg gtatccttgc cattggcgcg catcctgacg atatagaatt aggctgtggc
      361 gcatcgctag ctcgccttgc gcaaaaagga atttatattg ctaccgtggt gatgactacc
      421 ggtaattctg gcgtagatgg aataatcgat cgccacgaag aatcacgcaa cgctctaaag
      481 atattagggt gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag
      541 ctcaatgata tgatttctgc cctggaaaat atcataaaaa atcaaattcc ctctgatatt
      601 gaaattatac gtgtatatac catgcatgat gctgaccgcc atcaggatca tcttgcagtc
      661 tatcaagcct caatggttgc ctgtcgcgct attccgcaaa ttctcggcta tgaaacacca
      721 agtacctggc tttcatttat gcctcaggtt tttgaatccg tgaaagaaga atatttttct
      781 cttaaactga ctgcgttaaa aaaacataaa agtcagagcc agcgagatta tatgcgcccg
      841 gagcgtctgc gtgcagttgc tcaatttcgc gggcaacagg tcaatagcga tctgggtgaa
      901 ggttttgtta ttcataaaat gattctttga gcaaattgca tgaaaacctg gatctttata
      961 tgtatggccg tagcaatatt gctatggttt ctgagtacgt taaggcgtaa gcccagccaa
     1021 aagaaaggct gtattgacgc cattatacct gcttataacg aaggcccgtg tctggcgcag
     1081 tcactggata atctgctgcg taacccttat ttctgccggg taatttgcgt taacgacggc
     1141 tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt
     1201 attgccgtca cgcaaaaaaa taccggtaaa ggtggtgcgc taatgaatgg cctcaattac
     1261 gccacctgcg atcaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac
     1321 ggaatgggct atatgctggc agaaatagag cgcggtgctg atgccgtagg cggcattccc
     1381 tctactgcgt tgaaaggcgc gggtctgtta ccgcacatcc gcgcgaccgt aaagttgccg
     1441 atgattgtta tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt tattatcagc
     1501 ggtgcctgcg ggatgttccg tactgatgta ttgcgtaagt tcggtttctc tgatcgtact
     1561 aaagtcgaag accttgatct cacctggaca ttggtggcaa acggctatcg tattcggcag
     1621 gcgaatcgct gcatcgtata cccacaggaa tgcaacagcc cgcgtgagga atggcgtcgc
     1681 tggcggcgtt ggattgtggg ctacgcggtc tgtatgcgcc tgcataaaag acttttattt
     1741 agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgcttta tggcgttggg
     1801 atttatctca ctacctggtt taatgaattc atcaccaccg ggccacatgg tgtggtgttg
     1861 gcaatgttcc cgcttatctg ggtcggcgta gtttgtgtta ttggtgcatt tagcgcctgg
     1921 tttcatcgtt gctggttgtt ggtgccttta gcaccgcttt ccgttgtgta tgtattatta
     1981 gcttatgcca tctggattat ttatggactt attgcctttt ttactggacg cgaacctcag
     2041 cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaccct
     2101 tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa atattcagat
     2161 tgcagaccag gtgattattg atgaaagtgc cggtgaggtt gttatcggcg cgaatacccg
     2221 tatttgccac ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact gcctgatagg
     2281 taattatgcg tttattcgtc ctggcacaat aatcagcaat ggcgtaaaaa ttggttttgc
     2341 caccgaaatt aaaaatgcgg ttattgaagc ggaagcaacg attggtccgc aatgctttat
     2401 tgccgactcg gtagttgcaa accaggcata tttgggcgca caagtacgaa ccagtaatca
     2461 tcgtctggat gaacagcccg tgtctgttcg aactccagag ggaattatcg ctaccggatg
     2521 cgataaatta ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag ttattatttt
     2581 gcctgggcga attatttccc cgaacacgca acttggcccg cgcgtgattg tagaacgtaa
     2641 tttacctacc ggaacttatt cactccgaca agaacttatc cgtacaggag attaaaatga
     2701 taaaacggac gttattagcg gcggcaatat ttagcacatt gcccgcttat gccgggttaa
     2761 cttccattac cgcgggctac gattttaccg attattctgg cgatcatggc aaccgtaatt
     2821 tagcgtatgc tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt aatctttccc
     2881 aggggcgtcg tgattatgaa actgaacatt tcaatgccac tcgcggtcag ggtgcggtct
     2941 ggtataaatg gaataactgg ctgacaaccc gaacgggtat tgcctttgcg gataatacgc
     3001 cggtctttgc ccgccaggat tttcgtcagg atattaacct ggccctgttg ccaaaaacgc
     3061 ttttcacgac cggttatcgc tacactaaat attacgatga tgtcgaagtc gatgcctggc
     3121 aaggcggcgt atccctctat acaggcccgg tgatcaccag ctatcgctat acccattatg
     3181 actccagcga tgcaggtggt agttacagca atatgatttc cgtgcgtctg aatgacccgc
     3241 gcggcactgg ttatacgcaa ctatggctaa gccgcggaac aggtgcttac acctatgact
     3301 ggacgccaga aacacgctac ggcagcatga agagcgtcag tttgcaacgt attcaaccgc
     3361 tgactgagca acttaatctc gggctgacgg caggtaaagt gtggtacgac accccaaccg
     3421 atgattataa cggtctgcaa cttgcagccc atctgacctg gaaattctga ttccttctgc
     3481 cgcccgctat ccggggcggc cttccctgtc gattaggccc ctctttactt tttgttatcc
     3541 gactacattc accggataaa ttttattctc cagtgttata tactatagag gggtatgcat
     3601 tgacatataa catacccccc tatagtatat tgcgtgcaga tgatgaggtg cgaaatgccc
     3661 agtactccgg aagagaagaa aaaggtcctt actcgagttc gtcgtattcg ggggcagatt
     3721 gatgctctgg aacggtcgct ggagggtgat gccgaatgcc gtgccatact ccaacagatc
     3781 gctgccgttc ggggcgcggc taacggactg atggcagaag tgcttgaaag ccatatccgg
     3841 gaaacgtttg accgaaatga ctgctacagc cgcgaagtca gccaatccgt tgacgacact
     3901 attgaactgg ttcgtgccta tcttaaatag ctgaatctat taccatattg aggaagagcg
     3961 agagatgaaa tcacgtgctg ccgttgcatt tgctcccggt aaaccgctgg aaatcgttga
     4021 aattgacgtt gcaccaccga aaaaaggtga agtgctgatt aaagtcaccc ataccggtgt
     4081 ttgccatacc gacgcattta ccctctccgg cgatgacccg gaaggtgtct tcccggtggt
     4141 tctcggtcac gaaggggccg gggttgtagt tgaagtcggt gaaggcgtaa ccagcgtcaa
     4201 acctggcgac catgtgatcc cgctttacac cgcagagtgc ggcgagtgtg agttctgtcg
     4261 ttctggcaaa accaacctgt gtgtcgcggt tcgcgaaacg cagggcaaag gcttgatgcc
     4321 agacggcacc acccgttttt cttacaacgg gcagccgctt tatcactaca tgggatgctc
     4381 aacattcagt gaatacaccg tggtcgcgga agtgtctctg gccaaaatta atccagaagc
     4441 aaaccatgaa cacgtctgcc tgctgggctg tggcgtgacc accggtattg gcgcggtaca
     4501 caacacagct aaagtccagc caggtgattc tgttgccgtg tttggtcttg gcgcgattgg
     4561 tctggcagtg gttcagggcg cgcgtcaggc gaaagcggga cggattatcg ctatcgatac
     4621 caacccgaag aaattcgatc tggcgcgtcg cttcggtgcc accgactgca ttaacccgaa
     4681 tgactacgac aaaccgatta aagatgtcct gctggatatc aacaaatggg gtatcgacca
     4741 tacctttgaa tgcatcggta acgtcaacgt gatgcgtgcg gcgctggaaa gtgcgcaccg
     4801 cggctggggt cagtcggtga tcatcggggt agctggttct ggtcaggaaa tctccacccg
     4861 tccattccag ttggtcaccg gtcgcgtatg gaaaggttcc gcgtttggcg gcgtgaaagg
     4921 tcgttcccag ttaccgggta tggttgaaga tgcgatgaaa ggcgatatcg atctggaacc
     4981 gtttgtcacg cataccatga gcctggatga aattaatgac gcctttgacc tgatgcatga
     5041 aggcaaatcc attcgaaccg taattcgtta ctgatttccc gcaggtttac ccgtccagtt
     5101 cagacggggt tcttaatact ctccccgggc agccgtccgg gggattaacc gtgagataat
     5161 gactgatgga actcattgaa aaacatgcca gctttggcgg ctggcaaaat gtgtatcggc
     5221 attattcccc atcactgaaa tgtgaaatga atgtcggcgt ctatctcccg ccaaaagccg
     5281 caaatgaaaa attgccggtg ttgtactggc tttcaggcct gacctgcaac gagcagaatt
     5341 tcattactaa atcggggatg cagcgttacg cggctgagca caacattatt gttgttgcgc
     5401 cagacaccag tccgcgaggc agtcatgtcg cagatgccga ccgttacgat ctcgggcaag
     5461 gtgccgggtt ttacctgaac gcaacgcaag cgccgtggaa tgaacattac aaaatgtatg
     5521 actatatccg caacgagctg ccggatttag tgatgcaaca ttttccggca acgaccagaa
     5581 agtctatctc aggtcattct atgggcgggc tgggcgcgct ggtgctggcg ttacgtaacc
     5641 cagatgaata tgtcagcgtc tcggcgtttg cgcccattgt ctccccatcg caagtaccgt
     5701 ggggacagca agcctttacc gcatatcttg gtgaaaataa agatgcctgg ttggattacg
     5761 atccggtgag tcttatttca caaggtcaac gcgttgcgga aataatggtt gatcaggggt
     5821 tgagtgatga tttttacgca gaacagctgc ggactccaaa tcttgaaaag atctgccagg
     5881 agatgaatat caaaacgtta atccgttatc acgagggtta tgatcacagc tattattttg
     5941 tctccagttt tattggcgag catattgcgt accacgccaa taaactgaat atgcgttgag
     6001 catccgcgca cgcaggccgg atgcggcgta aacgccttat ccggcctaca tttcacccgt
     6061 aagccgtagg cctgataaga cgcgaaaagc gtcgcatcag gcatctgcgc acggctgccg
     6121 gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag gcctgataag
     6181 acgcgtaagc gtcgcatcag gcatcactgc acggcttatc tatgtttacc acatcaaata
     6241 atcaggcact ttgaagtcgg catacggatc gtcttcatcc tgctcttcgg cgctgagcgc
     6301 gctgtgcaac acaatactgc tggcatcacg ctgagcaatt ttatcggcaa cactggcggg
     6361 aataatggcg tattcacctt cgctattgtt atcgaccaac aagcgggcaa tcgccagacg
     6421 accattaatc aactgcgcct gtgtgagctt atcgacaaaa atcttcttaa tcagattgcc
     6481 gtcagtgaag ttaaaaccaa tatcgccatt ggcaatggtg attcggttca tttcaatgag
     6541 ctgcttcacc tgggctttat attctttcgc caacgccgct tgtttttgct gttcgctaag
     6601 ctgtttatca cgctcaagct gtgccttttt attttcttct accgccgccc gagcttcacg
     6661 cgcctgaacg cgcgattttt tcgccgttct ctcgaccttc gccgcttttt tgctggtgac
     6721 taatcctgct ttgagcaact gctcttgtaa ggtaagtttt gccatctttg tttccaaacc
     6781 cgctgaataa tttttagcga gtatacctgt aaacgccgta ggtgtattgg gggcaagtcc
     6841 ctttcgcccc tgcttaataa ggcgcgtcag gcatcggcgc acggctgcat tcgtgatctc
     6901 cggctcatca aaataaatac tgccaggcca gcaacaagaa tacccggcgc agaagcggcc
     6961 attacgccaa ccgtgccaat gcctaatgcc agcattttcc cggccagtaa cggaccgctc
     7021 atagccccca gacgccctac cgccacggct gttcccacac ctgttgcgcg gatctgcgaa
     7081 ctgtaaaaca acggtgccag ggcatacaaa acgctttgcc cacctgtcgc aaacaacccc
     7141 gcgacgaatc ccgccagcaa cataccgtta aatgacgaca ccgttcccag cgccagcagc
     7201 gaagctaaca tgccgctata aatcagtagc gacatggtta ctggacgcag cttatccatc
     7261 aatgcgccca acattaacgt cccgcttgcc gcccccattt gcagtgcaaa catcacccct
     7321 gccgcctgcg atggctggaa tccttgctcc accaaaagca gcggtagcca gttgatcaac
     7381 atgtagacca ccagcagagt gaagaaataa cacaaccaca gcagcagcgt cgcggttgcc
     7441 gtttctggcg caaataacgc acgcagtggt ggcgcagcct gtttttcgcc agcgaacacc
     7501 gccgactccg gcagccagcg cattaatagc ggcaccagaa tcaacggcac cacaccacct
     7561 acccaaaaca ccgtttgcca tgctaagttt gcccccgcga aacccagtgt cgccgccagc
     7621 gccgcgccaa tggggacacc gaaatacatc aggctcactg ccgtcccacg aaaacgtgga
     7681 cccgcggctt cagacgtcag ggcgatcaga ttcggcaacg ccgcccccag cccgacaccg
     7741 gtcatcagcc gcgcaaagac cagtgagggg aaatcccagg caatcgccgt tgccagtgag
     7801 aacaaaccaa acagcgcaac tgagccaatc aaaatgcgct tgcgaccata acggtccgcc
     7861 agcattccgc caaccaacgc gccgggtagc aaaccgagta ttccggcgct gaatatccag
     7921 cccatttgca ttttatcgag tgcgaaagcc tgggcgatgc cacccgccgc aatgccagcc
     7981 gcctgaagat ccagtccttc catcagagcg accaaaaaac aaagcccgat ggtcagcatc
     8041 aggcgggttg aagatgatga aggggtacga gtcgacataa gatgaacctc gttgcgagtg
     8101 caggtgaatc gcggctgata cattgtcagc cgcgtaatca gaaccattta atgactgcct
     8161 acgggcgaaa tataggccgg ataaggcgtt tacgccgcat ccggcagtcg tgctgggatg
     8221 ggcatacaaa atgcacgttc tcggcagtca tcattatttg ttgttgcgca gatccagcgc
     8281 cacgtcgacg atcatatcct cctggccgcc aaccatccgg cgtttgccca gctcaacgag
     8341 aatatccacc gcgcttaagc cataacgcgc cgccgccgtt tcacagtgac gcaggaagct
     8401 cgagtaaaca ccagcgtatc ccagcgccag cgtttcgcga tcgactcgta ccggtcgatc
     8461 ctgcaacgga cgcaccaggt cgtcagcggc atccattaac gcatagagat cggtcccatg
     8521 ctgccaaccc agtttatccg cggcggcaat aaaaacttcc agcggcgcgt tacctgcgcc
     8581 cgcgcccatt cccgcgaggc tggcgtcgat tcggtcgcag ccctcttcca ccgccgcgat
     8641 agagttcgcc acgccaagac tcaggttatg gtgagcgtgt atgccggttt gcgtttctgg
     8701 tttcagcact gctttcagag cgcggaaacg gtcacggata tcgctcatgt tcatcgcacc
     8761 gccagaatcc accacataaa tacaggtcgc accgtagcct tccatcagct ttgcctgctt
     8821 tgcgagattc tccggcgtgg tcatatggct catcatcaga aaaccaacgg tgtccattcc
     8881 gagctcgcgg gcatactgaa tatgctgggc ggaaacatca gcttcggtac agtgcgtagc
     8941 cacacgaacc acccgcgcgc cagcctgcca ggcatttttc agatcgtgaa tagtgccgat
     9001 tccaggcagc aacaacgtcg cgattttggc gtgcttcacc acatccgccg ccgcttcaat
     9061 ccattcaagg tcgctatgtg cgccgaaacc atagttaaag ctggaacctt gcaaaccgtc
     9121 gccgtgggcc acttcaatcg aatccacgcg ggcatcgtcc agtgctttgg caacctggcg
     9181 aacgttttcc aatgaatact gatgacgaat ggcgtgcata ccgtcacgca acgtgacgtc
     9241 cgagatataa agttttttac cgttcatgcc gcttctcctg ccttgcgcgc cagtgactgg
     9301 gccatttttt ccgctgtcgc cagcgcactg gaagtcataa tgtcgaggtt gcctgcatag
     9361 gcaggcagat aatgcgctgc gccttcgact tccagccaga ccgccgtttt cagtccggag
     9421 aactgcccca cgcccggcaa attaaccggt ttatcctgcg ggataacttc aaactgcacg
     9481 cgctgtttca ggcgataacc cggtacgtaa gcctgcaccg cctcagccat ttcattgatt
     9541 gaggcttcga tatcatcttg tgaagcttcg tcgctcaata catacaccgt gtcacgcatc
     9601 atcaacggtg gctctgctgg gttaagcaca ataatcgcct tcccttttgc cgcgccaccc
     9661 accacttcaa tggcgcgcga agtggtttcc gtaaattcat cgatattggc acgcgtgcca
     9721 ggtccggcag atttactggc aatagaagcg ataatttcgg cgtaatgaac acgcgccacg
     9781 cgtgaaactg ccgccaccat tggaatggtg gcctggccgc cgcaggtgac catgttgacg
     9841 ttcagttgag cgacgttctc ctcgaggtta acgaccggca cgcagtaggg gccgatggca
     9901 gcaggcgtca ggtcaattaa gcgaatatcc ggtttcgctt cgcgcaaagc ggcatcgttt
     9961 ttcacatgcg cacccgcgct ggtcgcatca aatacaatgt cgatatcagc aaattcaggc
    10021 atgttcatca ggccgatcac cccttcatgg gtggtggcga cgcccatacg tctggcacgc
    10081 gccagaccgt cggactgagg atcaatgccg accatcaccg ccatctccag atgctggccg
    10141 tgacgcaaaa ttttaatcat cagatcggtg ccaatgttgc ctgaaccgat aatggcgact
    10201 ttacgcttac tcatgacaga cttccttttg gggctgcgct tgaaaatgtc gcagcaactg
    10261 aacctatgcc ttcaatatgg gcttcaaaac gatcgcccgc attcaccgcc accatcggac
    10321 ctaatgcccc ggtaagaatg atatctccgg cgcgcagcgg ttcacccagg ctggccattt
    10381 tgcgtgccag ccagacggcg gcattaagcg gatgtcccag gcattcgctg ccgcgcccgc
    10441 tggaaacctc ttcgttatta cgcgtcatct tcatggcgca gtttttcagg tctaaccccg
    10501 ccggacgttg cgccggaccg ccgatgacat acaccccgca ggaagcgtta tctgccacgg
    10561 tatcgacaaa ctgaatcgac cagtcgcgaa tgcggctccc caccacttcc agcgccggaa
    10621 gtacccattc aatggcgtta tacaattcgt cgaaggtgat atcggttgcg ggtagatcgc
    10681 ggttcaacac cagtgcaatc tccgcttcaa tgcggggttg cagaacacgg gaaaaaggaa
    10741 tggtttcgtt atcgccataa cacatgtcgg caaataatgt cccgaaatcc ggttgatcaa
    10801 cgcccagttg ttgctgcact ttcggatgcg tcagccccac tttgcgcccc accactcgcc
    10861 gtccctgagc gacgtcgtgt tgcacattga tgtgctgaat ggcgtaggcc gcttcagcgt
    10921 tatcgatacc aatcagatcg cgcagcggcg caatcgcttc gccctgctct gcggcgcgac
    10981 gtaaatccgc cgccagttgc tcaagagtat gcttcgtcat gaccgttcct taagggcgtg
    11041 cgaggaaatt cagcaccagt tgattgaaag cgtcggcatg ttcccactgc gcccagtgac
    11101 cacagtcgcg gaagatatgc agttcagaac cggcaatgcc ggacagcaga cgcagacccg
    11161 catccatcgg cacaaagcgg tcgttgcgcc cccagacaat cagggtttgc gctttgattt
    11221 ccgccagacg tgggccaaaa tccgggaact gtttcggatt ggcttccagg ctcttaacga
    11281 agttttccag gtgatcgcgg cgcgacagca tattattcag gcgcgcttca aacagggcgt
    11341 cggtcaaatc gctggtatca aaaacgaaga tatccatcat cagcttcagg ttttcgatag
    11401 tcggctgacg ataaagctga ttcagtcgct taataccttc ggttggcatc ggcgtaaaca
    11461 aactcatgcc gcccgtaccg ccgcccatca gcaccagttt gccgacgcgc tccggccagt
    11521 taagggtgaa cgccacagaa ctgtggccgc ccatcgagtt gcccagcagg tggattttgg
    11581 cgatatccag ttgatccacc acgcttttca ggattcgcgc attaagatcc gatcgtgaac
    11641 cactattaac gatcgaatcg ctcttacccc aacccggaca atccagcagg atcacccgat
    11701 agcccgcctt taccagcgga tcgatattgc ggctgaagtt cgcccagcca gtagcacccg
    11761 ggccggaacc atgcagcagg acaacagttt cgtcgccttg tccgcagtca ttaaaatgga
    11821 tgcgcagcgt tttacccgct tcttctacat tcagaaaacg gctggtggcg gcttcggttt
    11881 gtggctgata actcatcatc ttctcctgca tattcagttc tctgttctgg cgcttaacga
    11941 gccaaatccg gcaatccact ccgggattgg gcggtaataa cgcccttcgc tacgccagtt
    12001 gccaaacgca gaaatagcgg caaaagcggc gacccaggtt ttgatttcat gtgtcgactt
    12061 tccggcaatg gcggaaagct cttcgttact gacggcatcc agttcctgta tgcgtccctg
    12121 ctccagcaaa gtcatgaact ggttatccca aatcgggttg agcggatgca gcgttctctg
    12181 atcctcaaca aacttctcag cggcgctaat cacccgttgc tgacgcaatt cgcgctcact
    12241 ggcgggtaaa tctttcccgc tccccaacag acggtcgcgc atatgggcat cggctttcgc
    12301 cagttctggc accggcggct gatgggaaag cccaccggaa cccaggaaca gcacgcgttt
    12361 attgagagta ctggtgaaac gtccaatggc ttcccccaac atgcgggtac gctggaaacc
    12421 gggcaacggc gtggcgacac cgttgatgaa cacaggcaga accggcacct tatccagccc
    12481 accgagcagg aactccagcg gctgggcgaa cccgtggtcc acctgcatac agtaagaaac
    12541 ggcaagatcg attccgctct tcatgacggc atgcgcacag gcctccgcca gctcaacagg
    12601 cacgggcagc tctcctgccg cactaccgaa atcaccaatt gccgtcgctc caacgcctaa
    12661 acagaacggt ggcatcacgt catagaaaaa gccgttgtag tgatctggcg caaacagcac
    12721 caccaactca ggggagaaag ccgcaatacg ctcgcgggcg ctggcaatca cgccattgac
    12781 ctcatcgagc acctcttgcg ccgggtcgac atatcccacc agcggcgagt gggaaagaca
    12841 gtgaagataa gcgtgcatat caggctacct tttcgacaga aacgtcggca tcagggcggg
    12901 tcagcgtcat taccgacgcc agtttattca gcgtattacc cagagtttgc ggaatggcgg
    12961 tggcggcaac aaagcgatcc gggcgcatca ccaccagcga agcattgtgt tgcgcgaacc
    13021 agctacgcag gcgaccttgc gtatcgccca cgcgtagtac gccgtcgtgg ttatcctgtg
    13081 cggtatgaat ttgcacttcc ggcaccacct gaatgaagcg tgtgcccaac gcgcgccact
    13141 gctggatttg ctcgtcgctc atcccccaca gtggattgca tccccagcca attaccgcga
    13201 agttcgcgcc gatcgcgtta tcgagcagcg tcacgtcgcc gttttccagc gtgactttcg
    13261 gctgaataaa catcttgccg accggagagt gcttcgcttc gccctcacgc accagcgcac
    13321 cgccgtaata ttgcggcatc ggcttgaagc gcatttcgag gaagtagcgt tttactggcg
    13381 gcagataatt caacagccag gaaacgccgt cacgtaacgt accctgccag cgtttcggcg
    13441 gagccagcac gttgcccgcc gtcacggaca ggtcaatcat cgctttggcg tgatcgcgac
    13501 gttcttgttg ataggtatcg agcagcgcat cgcgggcttt cccctggata accaacgcca
    13561 gtttccatgc gaggttaaag gcgtcgcgca taccactgtt atagccctgc ccctgccaca
    13621 ccggcatgat gtgcgcggca tcgcccgcca gcagtacgcg atcaatacga aaacgttgcg
    13681 ccagtcgcgc gttgtgggtg tagacacgct ggcgaatcaa ttcaacattg tccggattag
    13741 gcagcacttt gcttaacagc ttgcgcatat tttgcggctc acgcagctgt tcttcggttt
    13801 ctcccggcat caccataaat tcaaagcgac gtaccgcatg aggcagcgcg gcagaaacat
    13861 acgggcgcac cggatcacaa cacaaataga tatgcggcgt acttaacgga tcgttggcga
    13921 tatctaccac aatccactga tttggcgcag ttttaccttc aaacggcaca ttcagggtgc
    13981 gacggacaaa acttgcccca ccatcacagg ccaccagcca ttgggctttg accgtttccc
    14041 gctgcccttc tgccgttttc aggtgcaagg tcacttcgtc atcttgctgg ctgaaggcct
    14101 ccagctcgcg ggaaaacagg cagcgcacat tcggaaaacg cgacaatcct tccagcatca
    14161 ccgcatcgac ctgaggctga ataaaggcgt tacggcgcgg ccagccaaat tcatcggtca
    14221 ttggctgaat atcagcaaaa cagcgacctt ttggggtgag aaaacgcatc gcgtgccacg
    14281 gcgtagtgtg cggcagaaca ttatcgacca ggccgaccga ctgcatggtg cgcagcgcct
    14341 cgtcatcaat accaatcgca cgcgggtagt cgatcaactt atcgagtttc tccaccacca
    14401 gcacgtcaat gcccatctgg ccgagatagt tcgccatcat cagcccgacc ggaccggcac
    14461 cagcgatcgc cacctgaacg ctatggttaa cagcaggctg gatgtcagga tgttgtattg
    14521 ccatttcagt acctcacgac tcggacaaaa tgccgttgcg cgcacagtac ggcgcaactt
    14581 attttgttaa aaacatgtaa atgatttttt attgtgcgct cagtatagga agggtgtttt
    14641 cggctacaat caaaacatgc ccgaatgtgc accaagtgca caacgttgtt ttaactatag
    14701 aaatgtcaat taatatgcag aacaatgagc agacggcata caaaaccgtg cgcggcttaa
    14761 cccgcggtct aatgttatta aatatgttaa ataaacttga tggcggtgcc agcgtcgggc
    14821 tgctggcgga actcagcggc ctgcatcgca ccactgtgcg gcgactgctg gagacgctgc
    14881 aggaagaggg atatgtccgg cgtagtccct ccgatgatag tttccgtttg accatcaaag
    14941 tgcggcaatt aagcgaagga tttcgtgacg aacagtggat ttctgcactg gcggccccac
    15001 tgctgggcga tctgttgcgc gaagtggtat ggccgacaga tgtgtccacc ctggatgttg
    15061 atgcaatggt ggtacgcgaa actacccatc gtttcagccg cttatctttt caccgggcaa
    15121 tggtcgggcg acggttaccg cttctgaaaa ccgcctcggg cctgacctgg ctggcctttt
    15181 gcccggaaca agaacgcaag gaattaatcg agatgttagc ctcccgcccc ggtgatgact
    15241 atcaactggc acgggaaccg ttaaagctgg aagccattct ggcgcgcgcg cgcaaagagg
    15301 ggtacggaca gaactaccgc ggctgggatc aagaagaaaa gatcgcctct attgccgtac
    15361 cgctgcgcag tgaacaacgg gtgattggct gtctgaatct ggtgtatatg gcgagcgcaa
    15421 tgaccattga acaggcagcg gaaaagcatc ttccggcgct acaacgggtg gcaaaacaga
    15481 tcgaagaagg ggttgaatcg caggctattc tggtggccgg acggcgaagc ggcgtgcatt
    15541 tacgttgaca ccatcgaatg gcgcaaaacc tttcgtggta tggcatgata gcgcccggaa
    15601 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgc tatacgatgt cgcagagtat
    15661 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggcctgcca cgtttctgcg
    15721 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
    15781 gcacaacaac tggcgggcaa gcagtcgttg ctgattggcg ttgccacctc cagtctggcc
    15841 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
    15901 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
    15961 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
    16021 gccattgctg tggaagctgc ctgcgctaat gttccggctt tatttcttga tgtctctgac
    16081 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
    16141 catctggtcg cattgggtca ccagcaaatc gcgctgttag caggtccatt aagttctgtc
    16201 tcggcgcgtc tgcgtctggc gggctggcat aaatatctca cacgcaatca aattcagccg
    16261 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
    16321 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
    16381 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
    16441 tacgacgata ccgaagacag ctcgtgttat atcccgccgt taaccaccat caaacaggat
    16501 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
    16561 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac ccttccgccc
    16621 aatacgcaaa ccgcttctcc ccaggcgttg gcagattctt taatgcagct ggcgcgacaa
    16681 atttcccgac tcgaaagcgg gcagtgagcg caacgcaatt aatgtgagtc agctcactca
    16741 ttaggcaccc caggctttac actttatgcg tccggctcgt atgttgtgtg gaattgtgag
    16801 cggataacaa tttcacacag gatacagcta tgactatgat tacggattca ctggccgtcg
    16861 tattacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcggcac
    16921 atcccccttt cgccagctgg cgtaatagca aagaggcccg caccgatcgc ccttcccaac
    16981 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
    17041 cggaaagctg gctggagtgc gatcttcctg acgccgatac tgtcgtcgtc ccctcaaact
    17101 ggcagatgca cggttacgac gcgcccatct acaccaacgt gacctatccc attacggtca
    17161 atccgccgtt tgttcccgcg gagaatccga caggttgtta ctcgctcaca tttaatgttg
    17221 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aattcggcgt
    17281 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagccgt ttgccgtctg
    17341 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtactgc
    17401 gctggagtga cggcagttat ctggaagatc aggatatgtg gcgaatgagc ggcattttcc
    17461 gtgacatctc gttgctgcac aaaccgacca cacaaatcag cgatttccat gttaccactc
    17521 gctttaatga tgatttcagc cgcgcggtac tggaggcaga agttcaggtg tacggcgagc
    17581 tgcgcgatga gctgcgggtg acggtttctt tgtggcaggg tgaaacgcag gtcgccagcg
    17641 gcaccacgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca
    17701 cgctacgtct gaacgtcgaa aacccggcgc tgtggagcgc cgaaatcccg aatatctatc
    17761 gtgcggtggt tgaactgcac actgccgacg gcacgctgat tgaagcagaa gcctgcgatg
    17821 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
    17881 tgattcgcgg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
    17941 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt
    18001 cgcattatcc gaaccatccg ctgtggtaca ccctgtgcga ccgctacggc ctgtatgtgg
    18061 tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc
    18121 cgcgctggtt accggccatg agcgaacgcg taacgcgaat ggtgctgcgc gatcgtaatc
    18181 acccgagtgt gatcatctgg tcgctgggga atgaatcggg ccacggcgct aatcacgacg
    18241 cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg
    18301 gagccgacac ctccgcaacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc
    18361 agccctttcc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgctg cctggagaac
    18421 tgcgcccgct gatcctttgc gaatatgccc acgcgatggg taacagtctt ggcggcttcg
    18481 ctaaatactg gcaggcattt cgtcagtacc cccgtttaca gggcggcttc gtctgggact
    18541 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtca gcttacggcg
    18601 gtgattttgg cgatacgcca aacgatcgcc agttctgcat gaacggtctg gtctttgccg
    18661 accgcacgcc gcatccggcg ctgacggaag caaaacacca gcagcagttt ttccagttcc
    18721 gtttatccgg gcgaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg
    18781 agctcctgca ctggtcggtg gcgctggatg gcaagccgct ggcaagcggt gaaatgcctc
    18841 tggatgttgc tccacaagat aaacagttga ttgaactgcc tgaactaccg cagccggaga
    18901 gcgccggaca actctggctt acggtacacg tagtgcaacc gaacgcgacc gcatggtcag
    18961 aagccggaca catcagcgcc tggcagcaat ggcgtctgac ggaaaacctc agcgtgacac
    19021 tcccctccgc gccccacgcc atcccgcaac tgaccaccag cgaaacggat ttttgcatcg
    19081 agctgggtaa taaacgttgg caatttaacc gccaatcagg ctttctttca cagatgtgga
    19141 ttggcgatga aaaacaactg ctgacgccgc tgcgcgatca gttcacccgc gcaccgctgg
    19201 ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtagaacgct
    19261 ggaaggcggc gggccattac cagaccgaag tggcgttgtt gcagtgcacg gcagatacac
    19321 ttgccgacgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat
    19381 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg
    19441 ttgaagtagc gagcgataca ccgcatccgg cacggattgg cctgacctgc cagctggcgc
    19501 aggtcgcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc
    19561 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg
    19621 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc
    19681 agtggcgcgg cgacttccag ttcaatatca gtcgctacag ccaacaacaa ctgatggaaa
    19741 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc
    19801 atatggggat tggtggcgac gactcctgga gcccgtcagt gtcggcggaa ttccagctga
    19861 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc
    19921 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact
    19981 tttggatgtt cggtttattc tttttctttt acttttttat catgggggcc tacttcccgt
    20041 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgataca ggtattattt
    20101 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca
    20161 aactcgggct gcgcaaatac ctgctgtgga ttattaccgg catgttagtg atgtttgcgc
    20221 cgttctttat ttttatcttc gggccactat tacaatacaa cattttagta ggatcgattg
    20281 ttggtggtat ttatcttggc ttttgtttta acgccggtgc gccagcagta gaggcattta
    20341 tcgagaaagt cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg tttggctgtg
    20401 ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat aatcagtttg
    20461 ttttctggct gggttctggc tgtgcactca tcctcgccgt tttgctcttt ttcgccaaaa
    20521 cggatgcgcc ctcttccgcc acggttgcca atgcggtagg tgccaaccat tcggcattta
    20581 gccttaagct ggcgctggaa ctgttcagac agccaaaact gtggtttttg tcactgtatg
    20641 ttatcggcgt ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat ttctttactt
    20701 cgttctttgc cacaggtgaa cagggtacgc gggtatttgg ctacgtaacg acaatgggcg
    20761 aattacttaa cgcctcgatt atgttcttcg cgccgctgat cattaatcgc atcggtggga
    20821 aaaacgccct gctgctggct ggcactatta tgtctgtacg tattattggc tcatcgttcg
    20881 ccacctcagc gctggaagtg gttattctga aaacgctgca tatgtttgaa gtaccgttcc
    20941 tgctggtggg ctgctttaaa tatattacca gccagtttga agtgcgtttt tcagcgacga
    21001 tttatctggt ctgtttctgc ttctttaagc aactggcgat gatttttatg tctgtactgg
    21061 cgggcaatat gtatgaaagc atcggtttcc agggcgctta tctggtgctg ggtctggtgg
    21121 cgttgggctt caccttaatt tccgtgttca cgcttagcgg ccccggtccg ctttctctac
    21181 tgcgtcgtca ggtgaatgaa gtcgcttaag caattaatgt cggatgcggc gcgagcgcct
    21241 tatccgacca acatatcagg acggaatgat cgcaatgaac atgccaatga ccgaaagaat
    21301 aaaagcaggc aagctattta ccgatatgtg cgaaggctta ccggaaaaaa gacttcgtgg
    21361 gaaaacgtta atgtatgagt ttaatcactc gcatccatca gaagttgaaa aaagagaaag
    21421 cctgattaaa gaaatgtttg ccacggtagg ggaaaacgcc tgggtagaac cacccgtcca
    21481 tttctcttac ggttccaaca tccatatagg ccgcaatttt tatgcaaatt tcaatttaac
    21541 cattgtagac gactacacgg taacgatcgg tgataacgta ttgattgcac ccaatgttac
    21601 tctttccgtt acgggacacc ctgtacacca tgaattgaga aaaaacggtg agatgtactc
    21661 ttttccgata acgattggca ataacgtctg gatcggaagt catgtggtca ttaatccagg
    21721 cgtcaccatc ggggataatt ctgttattgg cgcgggtagt gtcgtcataa aagacattcc
    21781 accaaacgtc gtggctgctg gcgttccttg ccgggttatt cgcgaaataa acgaccggga
    21841 taagcactat tattacaaag attataaagt tgaatcgtca gtttaaatta taaaaattgc
    21901 ctgatgcgct ccgcttatca ggcctacaag ttcagtgatc tacgtaggcc gcatccaacg
    21961 taaacaaagc gcaggaacaa gcgtcgcatc atgcctcttt gacccacagc tgcggaaaac
    22021 gtgctggtgc aaaacgcagg gttatgatca tcaacccaac gacgcacagc gcatgaaacg
    22081 cccagtccat caagtaattg ccactgatac tacgcagcac accagaaaac cacggggcaa
    22141 gcccggcgat gataaaaccg atcccctgca taaacgccac cagtttgcca gcaatagcgg
    22201 gttgcgcaga gtgatcgagt gccagcagca aacagagcgg aaatgcgccg cccagaccta
    22261 aaccacacac catcgcccag aagaccggca attgcagcgg cagccagata aagccgcaga
    22321 aacccaccag ttgtaacgcc agcgccagca tcaaaagttt gcgccgatcc tgatggcgag
    22381 ccatagcagg catcagcaat gcccccgcag cttgcccaag cgtcatcaac gccagcaagg
    22441 aaccgctgta ctgcgcgctg gcaccaatct caatatagaa agcgggtaac caggcaatca
    22501 ggctggcgta accaccgtta atcagaccga agtaaacacc cagcgtccac gcgcggggag
    22561 taaataccac gcgaaccgga gtggttgttg tcttgtgaga agaggcgacc tcgcgggcgt
    22621 tttgccacca ccaggcaaag agcgcaacaa cggcaggcag cgcccaccag gcgagtgttt
    22681 gataccaggt ttcgctatgt tgaactaacc agggcgtaat ggcggcacca agtccaccgc
    22741 cgcccattag agccgcggac cacagcccca tcaccagtgg cgtacggtgc tgaaaccgcc
    22801 gcttaattac cgaaggcatc actgcctgaa tgatgccgat ccccactcca ccaagcagtg
    22861 cgctgctaag cagcagcgca ctttgggggt aaagctcacg catcaatgca ccgacggcaa
    22921 tcagtaacaa actgatggcg acgctgcgac gttcgctgac atgctgatga agccagcttc
    22981 cggccagcgc cagcccgccc atggtaacca ccggcagagc ggtcaacagg gcagccacgc
    23041 taaagctcat tccgcttgcc tggcgcaatt gcggtagcag tggcccgacg gaggtgagca
    23101 gtggtcgcat attaagacca atcagcacca gaaccagcag catcgtgaac gtcttttgat
    23161 ggttacgcat ggctgtcaga acggtttggt cggcagatat ttaccatcta aggtgatgac
    23221 cgcacgttcg ccaccctccg ggtccgccac tttcttaacg tcgagtttga agttaatcgc
    23281 gctgataatg ccatcgccaa acttctcatg aaccaacgct ttcagggttg tgccgtacac
    23341 ctgcaacatt tcatagaaac gatacatcgt tgggtcggtt ggaatacggt catcaatgca
    23401 gccacgcagt ggaatcatct gcaacagtag aatggcgtct tcgtcgagat ccagcttcgc
    23461 tccgaccagg cgggcggcgt cggcaggaag cgcctgctgc cccagcaaag ccgcggttac
    23521 aaaggcttct gccagaccgg tgccgtcggc aatctcggca aatgagagat cttttttagc
    23581 tttgctgagc aaaatggcat cggcaagatc aagacgaata ttgcggttaa tttgcgactg
    23641 aatcatggtg gatctcctga tggtttaaaa ataaggttac gctgcggtcg gttggcgtag
    23701 cggtatggca caaacgcgag gattagcggc cagtggcaca aactggcggg ttgcgccgtc
    23761 aaaagctgcg atgctgccgc tttcaatgtc gtagacccag ccgtgcaggg caatccgccc
    23821 ctcttcgagc gccaggcgca ccgatggatg agtttgcaaa ttagccaact gagcaatgac
    23881 gttttcacgt accatcgccg ctgcttttga cggtaaatcg ggatgcgggc gcgcctcatt
    23941 aacgacgcgg gctgaatcgg cataacgcag ccagtgggag acggcaggca tatggtccat
    24001 gcactgacag ctggcaatgg aggtcatcgc gccacagttg gaatgaccgc aaatcacaat
    24061 gtcagatacc cgaagtgcag cgacggcata ctccaccgaa gcagaaacgc caccgggttc
    24121 cggcccgtag gaagggacga tattgcccgc gttgcgaata acgaacagat cgccaggctc
    24181 acgttgcgtc accagctcag ggaccagacg gctgtcggag caggagataa aaagggtgcg
    24241 cgggctttgc tgtgtcgcca gctgtttaaa caaggcttcc cgcttcggaa atgcctcgcg
    24301 ctggaattta aggaatccat caataatctc tttcaccgtt aacctctgtc tgtctctgga
    24361 atgagaggca gactactcgc cgattgtcat aaggtaaaag actcatttat gatgagttct
    24421 attggattta cttataggtt ggcgaatgct ctctcgacat atcaattact ttcttgccgt
    24481 ggctgaacat ggcagcttca cccgtgcagc cagtgcgttg cacgtctccc agcctgcgct
    24541 ttcccagcag attcgccagt tagaggaaag tttgggcgtg ccgctgtttg accgtagcgg
    24601 gcgaacgatt cgtctcactg atgcaggaga agtctggcga cagtacgcca gccgggcgtt
    24661 acaggaactg ggggcgggta aacgggcgat tcatgatgtt gccgatctga cgcgaggatc
    24721 gctgcgtatc gccgtcactc ccacctttac aagctacttt atcggccctt taatggcgga
    24781 tttctatgcg cgctaccccg gcatcacgct ccagctacag gaaatgtcgc aggagaaaat
    24841 cgaagatctg ctttgccgcg acgagttgga cgttgggatt gccttcgcgc ctgtgcattc
    24901 gccggagctg gaggcaattc ctttactgac agaaagttta gcgttagtcg tggcgcaaca
    24961 tcatccgctg gctgcctgtg aacaggtggc gttgagtcgc ttgcatgatg aaaaactggt
    25021 cctgctcagc gcggaatttg ccaccagaga gcaaattgac cactactgcg agaaagcggg
    25081 gctacatcca caggtggtca ttgaggcgaa ctcaattagc gcggttctgg agctgattcg
    25141 ccgcacttcc ctttccacat tgttgccagc agcgattgcc acacaacatg acgggcttaa
    25201 agctatatcc cttgccccgc cactactgga gagaacggcg gttttgttgc ggcggaaaaa
    25261 tagctggcag acagccgccg cgaaggcatt tttgcacatg gcgttggata aatgcgcggt
    25321 tgttggcgga aatgaatcgc ggtaaccact gacacaattg cctgatgcgc tacgcttatc
    25381 aggcctacga gttcagtgct ctacgtaggc cggataaggc gttcacgccg catccggcat
    25441 aaactacgct cgctttgtca acacacagcc cagccgatca ccgtttgtaa tcgatggctt
    25501 ctggctgctc cagatatacg gtggtttgtg ctggttgggt gctggcaatc accttaccgc
    25561 cacgtaccga ataacgtaca ggaacctgac ggcgcagcgc gtcaaaccca ttttcagccg
    25621 gcaggataat caggttggcg ctgtttccgg cggcaatgcc gtaatcctgc aaattcaacg
    25681 tcctggcact gtggtgggtg attaaattca ggccatcgtt aatctgcccg tagcccatca
    25741 gctggcaaac atgcagcccc atatgcagca cttgcagcat attcgccgtt cccagcggat
    25801 accacggatc gaagacatca tcgtgaccaa agcagacgtt aatgccggac tccagcatct
    25861 ctttaacgcg cgtgatgccg cgacgttttg gataggtatc gaaacgtcct tgcagatgga
    25921 tattgaccag cgggttggcg acaaagttaa taccggacat tttcagcaag cggaacaggc
    25981 gtgaggtata cgccccgtta taggagtgca ttgccgtggt gtggctggcg gtgactcgcg
    26041 cgcccattcc ttcacggtgc gccagggcag caacggtttc gacaaagcgc gactgctcgt
    26101 catcgatctc atcacagtga acgtcgatga gacggtcgta tttttgcgcc agggcgaagg
    26161 ttttatgcag cgactccacg ccgtattcac gggtaaattc aaaatgcgga atcgccccca
    26221 ctacgtcagc ccctaagcgt aacgcctctt ccagcaacgc ttcaccgttg ggatacgaca
    26281 aaatcccttc ctgcgggaag gcgacgattt gcagatcaat ccacggcgcg acttcctgct
    26341 tcacttccag cattgctttc agcgcagtca gcgttgcatc cgaaacatcg acatgggtac
    26401 gcacatgctg aatgccgttg gcaatctgcc atttcagcgt ttgccaggcg cgttgtttca
    26461 catcgtcatg ggttaataac gctttgcgct cggcccagcg ttcaatgcct tcaaacagcg
    26521 tgccggactg attccagttc ggttgtccgg cggtttgcgt ggtgtccagg tgaatatgcg
    26581 gctccacaaa cggcggtata actaaacctt gttcggcatc caggctgttt tcagttatgg
    26641 gcatcacccc ggattgcgca tcaatggcgc tgatttttcc gtccagcaga tgaatctgcc
    26701 acagcccctc tttgccgggt aaccgggcgt taataattgt ctgtaaagcg ttattcgaca
    26761 ctgttagcct ccacatgcgt cattgcagcc gtcgttttgc gattcaaaat cgggttaagg
    26821 atcagatagc tcagcgcgcc acctaatacc gcgttgaccg gaacaatacc cggtaaccag
    26881 tggcctgcgg caatccccaa ggcgaccgcc agaatcgcca cccaattgac actcatcata
    26941 cgcgtggtcg caaagtgctc atagcggcga cggttcatca gatagtcagc gatgatcacg
    27001 ccccctaccg gaggaatagc tgccgaaagg aaggtcagcc agccgacaaa attgttatac
    27061 agccataatg cgcagaccgt accgataata ccgttgatta ccgaaagggt tttactcgac
    27121 ataccagtaa tgttggcgaa acctaaaccc gacgcataga gcgcgttatc gttggtggtc
    27181 cagatattca gccccagcac cacaatcgca ggcagcagca ggccctgagc aatcatcaca
    27241 tcagagatat ccgccatccc cagtgccgca gcgcccgctg caccgaaaat aaacatcaac
    27301 gagttgccga ggaaaaaggc caccatcgcc accagcaccg ccagtttggc attacgacca
    27361 aaccggacaa agtcagcggt gagcgtgccc gcactgataa atgaccccac aaccagcgcc
    27421 agcgcgacat tgaaatctaa cggttgtgcg ggaacgaccg cttttaatgc gtccaggccg
    27481 cccatgccat taacggccag ccacacggaa taaccgccaa ggcaggcgat agccggaacc
    27541 gcaatcaccg aaagaaccgt cagcgccgaa atgccaaaga agacggtgac ggtcatcagt
    27601 aaaccggaaa cggcaatcag caaattaata tccagcccgg ttgccttgcc caccggaatg
    27661 gcaaacatcg ccacgccaac gccaaaccag ccaacctggg tgccgcccag cagcagtgaa
    27721 ggcagccatg agcctttaac accaaaagag aagcgagcaa gaagatgagt ggtcaggccg
    27781 gtttttgcgc caatgtaacc aagaaatgaa gtgtaaatac cgaggagaag attaccgatg
    27841 aggactgcga ggaagaaatc atgatagcta agaccggttc cgagagtgcc gccggtccac
    27901 atactggcgg aaaagaaggt taatcccagc atgacgaacg tcaatgccaa tacccctttc
    27961 cgcgccgact gcgggactgg cccctggcta aagttgttat cttgcgacac gaaattcccc
    28021 ctttttgctg tttgaaaccc aaaaaatccg ccgcattcta ttcatctgaa gataaaaagc
    28081 aatcgttttc gtaggggaat atatttctta tatgaatgag tacaaaaagg cgggggaaac
    28141 ttatctgtgt aaaaacagtg ggtaggcatg ataagacgcg tcagcgtcgc atcaggcatc
    28201 tgcgca
//
LOCUS       DAZGAP010000050        27980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGAP010000050 DAZGAP010000000
VERSION     DAZGAP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.050"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            134..1858
                     /gene="yhgE"
                     /locus_tag="ACHLAM_001797"
     CDS             134..1858
                     /gene="yhgE"
                     /locus_tag="ACHLAM_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO7853332.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIIYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            complement(1996..2874)
                     /gene="hslO"
                     /locus_tag="ACHLAM_001798"
     CDS             complement(1996..2874)
                     /gene="hslO"
                     /locus_tag="ACHLAM_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO7853333.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(2899..3300)
                     /gene="hslR"
                     /locus_tag="ACHLAM_001799"
     CDS             complement(2899..3300)
                     /gene="hslR"
                     /locus_tag="ACHLAM_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO7853334.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(3311..3979)
                     /gene="yrfG"
                     /locus_tag="ACHLAM_001800"
     CDS             complement(3311..3979)
                     /gene="yrfG"
                     /locus_tag="ACHLAM_001800"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO7853335.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(4044..6179)
                     /gene="igaA"
                     /locus_tag="ACHLAM_001801"
     CDS             complement(4044..6179)
                     /gene="igaA"
                     /locus_tag="ACHLAM_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO7853336.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAHDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            6499..7059
                     /gene="nudE"
                     /locus_tag="ACHLAM_001802"
     CDS             6499..7059
                     /gene="nudE"
                     /locus_tag="ACHLAM_001802"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO7853337.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            complement(7227..9779)
                     /gene="mrcA"
                     /locus_tag="ACHLAM_001803"
     CDS             complement(7227..9779)
                     /gene="mrcA"
                     /locus_tag="ACHLAM_001803"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO7853338.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTDAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            9899..10668
                     /gene="hofM"
                     /locus_tag="ACHLAM_001804"
                     /pseudo
     CDS             9899..10668
                     /gene="hofM"
                     /locus_tag="ACHLAM_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            10668..11207
                     /gene="hofN"
                     /locus_tag="ACHLAM_001805"
     CDS             10668..11207
                     /gene="hofN"
                     /locus_tag="ACHLAM_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO7853339.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            11191..11631
                     /gene="hofO"
                     /locus_tag="ACHLAM_001806"
     CDS             11191..11631
                     /gene="hofO"
                     /locus_tag="ACHLAM_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO7853340.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            11621..12025
                     /gene="hofP"
                     /locus_tag="ACHLAM_001807"
     CDS             11621..12025
                     /gene="hofP"
                     /locus_tag="ACHLAM_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO7853341.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            11937..13175
                     /gene="hofQ"
                     /locus_tag="ACHLAM_001808"
     CDS             11937..13175
                     /gene="hofQ"
                     /locus_tag="ACHLAM_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO7853342.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            13576..14097
                     /gene="aroK"
                     /locus_tag="ACHLAM_001809"
     CDS             13576..14097
                     /gene="aroK"
                     /locus_tag="ACHLAM_001809"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO7853343.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            14154..15242
                     /gene="aroB"
                     /locus_tag="ACHLAM_001810"
     CDS             14154..15242
                     /gene="aroB"
                     /locus_tag="ACHLAM_001810"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO7853344.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            15334..16620
                     /gene="damX"
                     /locus_tag="ACHLAM_001811"
     CDS             15334..16620
                     /gene="damX"
                     /locus_tag="ACHLAM_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO7853345.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            16727..17563
                     /gene="dam"
                     /locus_tag="ACHLAM_001812"
     CDS             16727..17563
                     /gene="dam"
                     /locus_tag="ACHLAM_001812"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7853346.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            17581..18258
                     /gene="rpe"
                     /locus_tag="ACHLAM_001813"
     CDS             17581..18258
                     /gene="rpe"
                     /locus_tag="ACHLAM_001813"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO7853347.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGIGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            18251..19009
                     /gene="gph"
                     /locus_tag="ACHLAM_001814"
     CDS             18251..19009
                     /gene="gph"
                     /locus_tag="ACHLAM_001814"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO7853348.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            19002..20006
                     /gene="trpS"
                     /locus_tag="ACHLAM_001815"
     CDS             19002..20006
                     /gene="trpS"
                     /locus_tag="ACHLAM_001815"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO7853349.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            20296..21201
                     /gene="yhfZ"
                     /locus_tag="ACHLAM_001816"
     CDS             20296..21201
                     /gene="yhfZ"
                     /locus_tag="ACHLAM_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO7853350.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            21218..21580
                     /gene="yhfY"
                     /locus_tag="ACHLAM_001817"
     CDS             21218..21580
                     /gene="yhfY"
                     /locus_tag="ACHLAM_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO7853351.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            21664..22827
                     /gene="yhfX"
                     /locus_tag="ACHLAM_001818"
     CDS             21664..22827
                     /gene="yhfX"
                     /locus_tag="ACHLAM_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO7853352.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            22827..24053
                     /gene="yhfW"
                     /locus_tag="ACHLAM_001819"
     CDS             22827..24053
                     /gene="yhfW"
                     /locus_tag="ACHLAM_001819"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7853353.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            24050..24928
                     /gene="php"
                     /locus_tag="ACHLAM_001820"
     CDS             24050..24928
                     /gene="php"
                     /locus_tag="ACHLAM_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO7853354.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNIVKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            24939..25292
                     /gene="yhfU"
                     /locus_tag="ACHLAM_001821"
     CDS             24939..25292
                     /gene="yhfU"
                     /locus_tag="ACHLAM_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO7853355.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            25304..26608
                     /gene="yhfT"
                     /locus_tag="ACHLAM_001822"
     CDS             25304..26608
                     /gene="yhfT"
                     /locus_tag="ACHLAM_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO7853356.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            26620..27705
                     /gene="yhfS"
                     /locus_tag="ACHLAM_001823"
     CDS             26620..27705
                     /gene="yhfS"
                     /locus_tag="ACHLAM_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7853357.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(27812..27979)
                     /gene="yhfL"
                     /locus_tag="ACHLAM_001824"
     CDS             complement(27812..27979)
                     /gene="yhfL"
                     /locus_tag="ACHLAM_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO7853358.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6549 a         6920 c         7716 g         6795 t
ORIGIN      
        1 tgaaattgat gcaaattcaa tcggtaaagt atcgattcac cacgctaact ttttttgatt
       61 attttctgaa aaaagcaaaa tactacggcc cggatgcaat aattaaccca ctttttgatt
      121 ctggataact aacatggata acgttgaact ttcaccggca acacgctggg ggatgattgc
      181 taccggatta cttcagggtc tggtttgcta cctgctgatc gcctggcttg ccggaaaaaa
      241 tcacagttgg attgtttatg gcgtgcctgc gaccgtcgcg ttttcatccg ttttactctt
      301 ctccgtgatc tcctttaaac agaagcgcct ttggggatgg ctggcgctgg tgtttattgc
      361 cacgctaggg atgagcggtt ggctaaagtg gcaaattgat ggcatgaacc cctggagagc
      421 agaaaaggcg ctttgggatt ttggttgcta tctgctgctg atggcaatgt tgttgctacc
      481 gtggatacaa caaagccttc gcatccgcaa tgacagtagc cgctatcgct atttttacca
      541 gtcagtatgg cataacgtac ttatattact ggtgattttt ctcgccaatg gcctgacgtg
      601 gctggtactt ttactgtggg gagagttgtt taaacttgtt ggcatcacgt ttttcaaaac
      661 gctctttttt gcaaccgact ggtttattta tctcacgtta ggtctggtta ccgcactggc
      721 ggtgatcctc gcgcgtacac agtcacgttt aatcgactct attcaaaagt tgttcacgtt
      781 aatcgccacg gggttgctgc cgttagtatc attgctaacc ctgatgttta ttatcaccct
      841 gccgtttacg ggcctgagcg cgatttctcg ccacatctcc gccgctgggt tgctgttaac
      901 gctggccttt ttgcaattga tcttaatggc tattgtccgc gatccgcaaa aagcgtctct
      961 tccctggacg gggccgttgc gttgcctgat taaaaccgct ttgctggttg ctccgctgta
     1021 tgtgttcgtc gccgcctggg cgttatggct gcgggtcgct cagtacggct ggactgtcga
     1081 ccgcttgcag ggcgcgctgg cggtgctggt gttactggta tggtcgctgg ggtattttgt
     1141 cagtatcgtc tggcgtaaag ggcaaaaccc ccttgttctt cagggcaaag tgaaccttgc
     1201 ggtttcgtta ttggtgttgg taatactggt gcttcttaat tctccggtgc tggacagtat
     1261 gcgcattagc gtgaacagtc atatggcgcg ttatcagagc ggcaaaaaca cgccagacca
     1321 ggtaagtctc tatatgctcg aacagagcgg tcgctatgga cgtgcggcgc ttgagtcgct
     1381 gaaaagcgat gccgagtata tgaaagaccc gaaacgcagg cggggtctgc tgatggcatt
     1441 tgatggagta caacgtcttc agcagcagat atcggaaaaa gcattagcag ataatgtatt
     1501 tattgcctca ggttctggaa aaccggatac gatattctgg tcggcagtga tgaaaaatcg
     1561 ctataacgca atgatatgca ttgaaaaaga tgcctgcgtg ctggtagagc aagatctgaa
     1621 tagtgatggc agggcagagc ggatcctgtt tgcctttaat gatgaaagat acattatcta
     1681 tggtattgat cctgctggga aagaatggaa agagattacg atgaattcac tcccggctgt
     1741 aataacgaaa gaaaaattac tcatggctgc ggaggatgga aaactgggga cgaagcctaa
     1801 agtgtggcgc gatcttacgg tggatggcga aagactggat ttgaatctga atgaatgaca
     1861 gcgcatatct gatttttatt ggattcggca cattatctta cctgcgataa attgcattga
     1921 atccgtaggt ctgataagcg catcaggcca ttacgtcgta aaatccggca gagccctctc
     1981 tgccggacat actcattaat gaacttgcgg atctgccgga gacgcgttgt tgcggatttc
     2041 agcaatatcc atcgcattga acagatagtg gttaccgcag taatcacaat gcatgtcaat
     2101 ttcgccatct tccgccagga tgctatcgac ttcttcatcc ggcagcgttt ttagcgcgtc
     2161 ggcgcaacgt tcacgcgagc aggtgcattt gaactccaca tcctgcggat cataaaccgt
     2221 cacctcttct tcgtgataca aacgccacaa cacttcgttt gccggtaagg tcaacagttc
     2281 ttcggttttg atggtttcgg ttagcgtcgc caggtggtca aagtcgtcct gctgggcatt
     2341 ttgcgcaggc attacctgca acaacatacc gcctgcagcc ggtttgccgt ctacgtcgcc
     2401 ggtgcgaata aacaggcgcg tcggcagctg ttcagaacgc ataaagtaat cttccaggca
     2461 ggccgccaga gtatcacctt ccagacccac cacgccctga tagcgttcac cttcgctcgg
     2521 ggtgatggtg atcaccacgt aaccattgcc gaccagcgtt ttcaggtcgg cattttctgg
     2581 aatttcgccc tgcacgcgcg ccacaccgcg catctgctgg ttgttgttac cgttaataac
     2641 cgccagattc atcggaccgt cgccttgcag ctgtacggtg atatcaccat caaacttcag
     2701 cgtagcggtt aacaggctgg tcgcaaccag cagttctgcc agcacgtttt taacgggctg
     2761 cggataatcg tggttctcaa ggatctgttg cagggtttcc gaaacggtta ccagttcgcc
     2821 gcgaacggca aagttttcaa acagatagcg atgtaattgg tcatgttgcg gcataatcat
     2881 ctctcttgca ggtgacagtt attcactgtc gccgtgttta aatcgtaaca ggtcgcggcg
     2941 ctcttttttg tccgggcgtc ggtccgggtg cggcatggtt aaggcattaa gtttacgtgc
     3001 cagcgccatt ttttcgcgtt tctctacact ttccgcagtc tcttcataca gcaaggctgc
     3061 ctcgctggcg gggcgacgct gttcagtaat cgcctttaca atcaccgtgc gttcgtcatt
     3121 tccctggcgc agagtgagtg tggcattcag ctcgacgatt ttgctcggct tgctgcgctg
     3181 cccgttgtaa tgcaccttac cgccttcaat catttcacgg gccagcgcgc gggttttata
     3241 aaaacgggca gcccatagcc atttatccag tcgaacctca acagcaggtt tttctttcat
     3301 ggcgtctcct tcacattagc gaggggatca ggcggcggta gtcattcagt gacggatggc
     3361 gttgatactg tttctcggca atcccggagt caggattagt cacgccgagg cagtaacgaa
     3421 taccaaattg cgcggcagca tcgagaatcg cttcgctgtc atcaataaac agcgttcttt
     3481 cagctttcag acccgtagct tcggccaccg catgccataa ccgctgatcc tctttcggat
     3541 aaccaaatgt gtgggtggaa agtaataaat caaggtgtgc gtccagaccg gtatgctcaa
     3601 gttttaccgc caggttgtgc ggatgcgcat tggtgagcaa aattcgctgc ttaccgctgg
     3661 ctttcagtgc ctcaagaaac ggaatggtat cttcacgcag tacggcgcgc ggtcccatct
     3721 cggtggtcat cgcacagata tccagaccca gttgctcact ccagtaatca agacagtacc
     3781 agtttagcgt atgctgtacg tcgtgatatt gctggcgcat atattccatc gcttcctgtg
     3841 gcgtaacccc gtttttcgcg ccccatgttt caggcaccag cttttgccag aaatagttat
     3901 cgaaggcgag gtcgagcaac gtgccgtcca tatccagcag aacggtatct acgtcctgcc
     3961 aggcaatgtt gatatgcatg agggaaatct ccagagtgaa gcaatttgcg cgacagggta
     4021 gcataacctg ccgcgcaaac gtgttattcg ataaggcttt ctgaaggggt gatcagttgt
     4081 gggttcaggc agctttcata ataggcctga atcttcatca tgcgagtacg atgacgctgg
     4141 taacgccgcc aggcctgtac gccattataa atggcgctac ccagcatcgt cagtagcagc
     4201 agcgtggtgc tgagatagcg ccacaggccg gaacgatccg ggatcggatg aaggcctata
     4261 tgctgcgtac cattggcgtc agtgaagatt tttgtgacga taccctcggc gttaaacggc
     4321 gtatgcatca gcatttgtgc cagtttctgg aaagcgttcc actgttcttg cggcgggtag
     4381 tcgtaaagtg atgccgaagg ccagggctga tcaacaaaat cgctgccttc gtcgctgaca
     4441 atcaggaatc cgccgggggc cggactgttt agtgattgtg ccgctcgcgc cgtttcatgc
     4501 gtgataaacg gcgcggtgga ggttgccacc aggttatcca gcgattccgc actcaccggg
     4561 cgtaataaca cattcacgcc atcgagtttc ccggcgttgg cgcgttttac cagcgcgtcc
     4621 cagtctttac tgttgccgag attgaccagc gcatttttca gtcgcacaca gtcatctttg
     4681 gcagaacaca gatccgctgt cttcagtact atgtcgccaa aatcatcaag caataccatg
     4741 ccggattttt gaattgctga acgtaatgag gcgctgacgc gagattcatc ttccggttta
     4801 gggtgcagct ggcgattaac tgcttcggtc aatgccgtcg ctttgttgac caattcagat
     4861 tctggtaatg gcaatgagcg ggcgtcattc cagatgatct gcgagcagtc aaacggtaaa
     4921 aaaggtgagt tggttttcgc gctccaggta ccggaagtgc gaatattaca cattcccgta
     4981 ccgctaatac gcaatgtatc gccaacccgt acgccagcgt cagccagttg tttcacgctg
     5041 gtggcttcaa tggtctgcgc gcctttcatc catgagagag tgaatttcag cggcatatcc
     5101 agcgggatcc agaataacag cataaacagc accagcagcg aacccgcagc gataatcgta
     5161 ctgcgcagcc agtgctgtaa cggaaagttt tttacttcat catgcagcga aagatatcgc
     5221 ccctgacgca ctacgtggcg gtcgagatag atatcgatat cggtttgttg gccgagatca
     5281 tgagcaatgt agggctgcca gtgtgcgggg tagaccaggt cgataatacc gagtgaaata
     5341 ttgttgatct gttcctgatc gttttcgcca aacaatcccc aacgacgggg tgtaccgcgc
     5401 agacaatgaa tttcccgcag ggaggatttt gccgggggcg cgaatagccc ccacagacct
     5461 gcgcccagca gcagtaacgc gccgcctgcc agccacggaa caaatacatc cggggtaatc
     5521 aggcagaaaa agaacatcag gaaggaggcg acaatcagca acgcttcgcg cagcccgcgc
     5581 ggacgactta gcgcatactc ttcgtgcgtt tctttgcgaa tattgagtag ctcgatttgc
     5641 tcactctctt ccccgcgaat cgacgcctgt gttgacggaa caggttgcaa ggcatagccg
     5701 cgagtttcct gcatgtactc ctgcagcgta tgaccgttga gtgaaataac cagcggcagc
     5761 gaatcggtat gaatcagttc aacggtattc tcatcgttga tgtactgctc ccagaaaggg
     5821 ggcaggtgga cttctaccga atcgaggtag taacgccatt tattcggatc gtcggtagag
     5881 atgccgtaac gcgtgatagc gtgtgttagc atcatgacgt tgttgctttc ggcattcagc
     5941 gccagagaaa tcggtgccgc gctggctccc gttgggccag gcacctgtaa tacctgcgtc
     6001 aggctctcaa gataattttc aacggcgcta cgttcttcag gtgtgagttt acgcgtttgc
     6061 gcatccgcga aggcgttggt ccagggcagc tgacgccgtc tggatcgcac ttttatcagc
     6121 catcccgcaa gtagtgagca ggccagcaaa gcagctaaaa aaatcacaat ggtgctcatg
     6181 ctttccccat cttacttagt ctgtcaggcg tggtcagtcg caccggacac aaaacatgat
     6241 tctgcggttg gctatcggca agcgtggagt ttaacttgag catcattaag cacatcacag
     6301 taaccgctga tcatagcaaa ccctctgaag gcggaatatc agcaaattcc tggagttatt
     6361 tcaacctatt gacttttaat ttgatttgag gacgattatt tattaagtta cataaatgta
     6421 aataacatgc aattcacatt gccgaaagcg tgtccgatat cgcacaataa ccgctgattt
     6481 cacagcatag atcccacgat gagcaaatca ttacaaaaac ccaccattct gaatgttgaa
     6541 actgtagccc gttcccgact gtttaccgtc gaaagcgtgg atctggagtt cagcaatggc
     6601 gtgcggcgtg tttatgaacg aatgcgtcca accaaccggg aagcagtgat gattgtgccg
     6661 attgtggacg atcacctgat cctgatccgc gaatacgcag tgggaactga atcctacgaa
     6721 ttaggttttt cgaaaggatt aattgatccg ggtgaaagcg tcttcgaagc cgcgaaccgc
     6781 gagctaaaag aagaggtggg atttggtgcg aacgatctga cttttttgaa gaagctcagc
     6841 atggcaccgt cttacttttc cagcaaaatg aatatcgtgg tagcgcaaga tctctacccg
     6901 gaatcgctgg aaggcgatga gccagagccg ctaccacagg tgcgctggcc gctggcgcat
     6961 atgatggatt tgctggaaga ccctgacttc aacgaagcgc gtaatgtcag cgcgcttttc
     7021 ctcgtgcgcg aatggttgaa ggggcagggg cggttgtaaa tgcgtgaagt ttgcctgatg
     7081 cataatgctt atcaggcctg tgtggctcct gcaatatatt gatttttcta gatgttttta
     7141 gacgggtaaa gtgttcacgc cgtatccggt ataaacaaag tgcactttgt cagcaaactt
     7201 aaaaaggcgc tgaagcgcct ttttaatcag aacaattcct gtgcctcgcc attatcgata
     7261 atggtcgttc ccacctcgtg caccgcctgt tgtgtcggct gcgtaccttc gatgaaatac
     7321 tcttcgcggc tgttgccacc attagctaac tgcccggtgc tgcgatcgat attcaccgtc
     7381 acaatacccg gcggcggcgt cagcggctgc tccggcacac cttcaagaac ggctttcata
     7441 taagcgtccc atgcaggttg ggcactcttg gcaccgcctt cgtaacctga gatctgatct
     7501 ttaatcgctc cggaagctgt tgtatgaccg agattacgac ggtgatcatc aaagccaatc
     7561 cagaccgagg tcacaacgcc cggaccgtaa cccgagaacc acgcatcttt cgaactgtta
     7621 gtggtacccg tttttccgcc tatatcatgc cgttgtaaat cacgacctgc tcgccagcca
     7681 gtaccctgcc agccgggttc accaaaaata ttggtgttca aggcactctt aatcaggaat
     7741 gccagcggag tgttgatgac gtgcggtgcg tattcctgcg cgtcggtctt cgccactaac
     7801 gcctgatttg cctgctccag ctgcggcatt ggtacagaaa cattctgctg ctcgcgggag
     7861 atagcgacat cttcaacatc gttattttcc agcacgttcg atttctgcgt atcaccgtaa
     7921 atcaccggaa tatcgcattc cgggcaggct actttcggtt tcgcttcgaa aatcacgccg
     7981 ccctgatcgt tttcaatttt gctgataaac cacgggtcca ccaggaagcc gccgttcgcc
     8041 atgaccgcgt agccgcgcgc cacctgcatt ggggtgaatg acgctgaacc cagcgccagc
     8101 gattcggtgt ggacaatgtt ttgcgccggg aagccgaagc gttgcagata ttctgcagcg
     8161 tagtcgacgc ccatcgcccg cattgcgcgt accatcacca cgtttttcga ctggcccagt
     8221 ccctgacgta agcgaattgg accggcatac tgcggtggtg agttcttcgg ctgccagtca
     8281 gaaccggcac ctgcatccca gcgggaaatt ggcacatcgt tcaacatact tgccagcgtc
     8341 agacctttat ccatcgccgc ggtgtagagg aacggtttga tgttggaacc tacctgacgc
     8401 agtgcctggg tggcgcggtt aaacttgctc tgattgaaat caaagccacc gaccagcgcc
     8461 ataacggcac cgttttgcgg attgatcgac accagcgccg agttcacttc cggcacttgc
     8521 gccagccacc atgcatcgcc aacctgacga acccagattt gctgacccgt ttgcagaaca
     8581 tcggtcactt tacgcggcgt cggtccctgc tgagtatccg aacggtaagg acgcgcccag
     8641 cgaacgcctt ccatactcaa tgcgacggtc gacccgtccg ccagcatcgc cgtcgcttcc
     8701 tgaggattgg cgctggtgac tgcggcaggc agcagcggac cataggtcgg cagcgccttc
     8761 agcgtatcgg taatcttgtt gttatcccac gccgactcgc ccactttcca cagcacattt
     8821 gccgggccgc gatagccgtg gcgcatgtcg tagtccagca cgttattacg taccgcctgc
     8881 tgtgcggcct gctgcacttt gcgggtgatg gtggtgtaaa tgcgataacc gtcttcatag
     8941 gcactttcgc cataacggtt atacatctcc tggcgcacca tttcgctcag gtacggcgca
     9001 gagaaagcaa tctccggcgc gtgatagtta gcgttaatcg cctcagtgcg tgtctgatcg
     9061 aactgttgtt gggtgatata cccttcatcc agcatccgcg acagtacgac gttacgccgc
     9121 gcgacggcac gatccattga gtagagcgga ttaaaggtgg aaggcgcttt cggcagcccg
     9181 gctatcaccg ccatttcgtt cagcgtcagt tggtcgaccg ttttaccgaa atagacttgt
     9241 gccgcagcac cgacaccata ggcgcggtaa ccgaggtaaa tcttgttcag ataaagctcg
     9301 aggatctcgt ctttcgtcag caactgttca atgcgaatcg cgaggaagac ttccttaatc
     9361 ttacgcatca gcgtgcgttc tggactgagg aagaagtttc tcgccagctg ctgggtaatg
     9421 gtactcgccc cttgtgacgc gtggccggag aacagcgcca cacttgccgc acggaagatc
     9481 cccaccgggt caacgccgtg atgctcgtag aagcggctgt cttccgtcgc gataaaggct
     9541 ttcaccatct ccggcgggat ttgatccaac gtaaccggaa tacgacgttt ctcaccgtat
     9601 tgagcaatca gctcgccatc ggcgctgtaa atctgcatcg gaatttgcag gcgaacatct
     9661 tttaatgtcg ccacatccgg cagttgtggc tcgatgtagc ggtataggcc ataaatcgag
     9721 cctgctccca gcagaatgca acagactgca aggatcaaaa aatactttac gaacttcact
     9781 ggaaatttcc catttagttt catttgggca gtttataaac aaacgcgcgg tagtataaag
     9841 gcaagccaga cgcattgata tacccgtcag agtgacgggt gataaggaga tcatcacaat
     9901 ggcatttaag atctggcaaa ttggtttgca tttacaacag caagaagcgg tagcggttgg
     9961 ggcacaaaag aatgcttttt gcaacgctgg tggcggttgc cgctggagaa cgacattatc
    10021 aaagatgggc ggattgttga tgtgcagcgg ctggctaaaa cgttgttacc gtggagtcgc
    10081 gaactgccgc agcgtcatca cattatgttg gcgtttcccg ccagtcgcac attacagcgg
    10141 tcatttccac gtccgtcgat gtcccttggt gagcgggagc aaacggcctg gctgtccggg
    10201 acgatggccc gcgagctgga tatggatccg gactccctgc gcttcgatta cagcgaagac
    10261 tcactcagtc ccgcctataa cgtgactgcc gcgcaaagca aagagttggc aacgctgctt
    10321 actctggcag aaaggttgcg tgttcatgtt agcgcgatca ccccggatgc cagtgcatta
    10381 cagcgattcc tgcctttttt accttctcat cagcaatgtc tggcctggcg tgataacgaa
    10441 cagtggctgt gggcgacgcg ctatcgctgg gggcgcaaac tggcggtggg gatgactagc
    10501 gcgaaggagc tggcggcagc gttatccgtt gatcccgaaa gcgtcgcgat atgtggcgaa
    10561 ggcggatttg atccctggga ggcggtttct gttcgtcagc cgccgctacc gccgccaggt
    10621 ggagactttg ccatcgcgct ggggctggcg ctagggaagg cgtactgatg aacccgccaa
    10681 ttaatttttt gccctggcga cagcaacgcc ggaccgcttt tctgcgtttc tggttgctga
    10741 tgttcgttgc gcctctgctg ctggctgtcg ggataacgct aatactgcgt ctgacaagca
    10801 acgccgaagc acgcgtagac accgttttgc ttcaggcgga acaacaactc gcccgcagct
    10861 tacagataac gaaaccacgt ttgctggaac gccagcagtt acgcgaacag cgtttgcaaa
    10921 ggcagctcca gcgacaattt acccgtgact ggcaatctgc gctggaagca ctggcggcgc
    10981 ttttacccga acatgcctgg ctgacaacga taagctggca gcaaggtacg ctggagatca
    11041 aggggctgac aacaagcatt accgcgttaa acgcgctgga aacgtcactc cgccaggatg
    11101 catcttttca tctcaatcag cggggggcca cgcagcagga tgcgcaggga cgctggcaat
    11161 ttgagtatca gttaacaagg aaggttagcg atggacatgc tctttgactg gtggttcgcc
    11221 acatcacccc gcctccgcca gctttgctgg gcagtctggt tgctgatgtt agttacgctc
    11281 atttttctgt catcgacaca ccatgaagag agcgacgcat taattcgact acgggcaagt
    11341 catcaccagc agtgggccgc actgtatcgc ctggttgaca ccactccctt cagcgaggaa
    11401 aaaacgctgc ccttttcgcc actggatttt cagttatccg gcgcgcaact ggtttactgg
    11461 catccatccg cgcagggagg tgagttggcg ttgaaaacgc tgtgggaagc agtgccgtcg
    11521 gcatttacac ggctggcaga gcgcaacgtc agcgtgagcc gtttttcgtt aagcgtggaa
    11581 ggtgatgacc ttttgttcac gctacaactg gagacgccgc atgagggtta aacgctggtt
    11641 gttggcaggt attacattgt gccttttaac cggtatgcgt gaccctttta aaccgccgga
    11701 agatctatgc cggattagcg aacttagcca gtggcgctat caggggatgg tagggcgagg
    11761 tgagcgcatc atcggtgtaa taaaagacgg gcaaaaaaaa tggcgacggg tgcagcaaaa
    11821 cgacgtgctg gaaaacggct ggacaatttt acagctgacg ccagacgcac taacgctggg
    11881 aaccgggaca aactgcgaac cgccacactg gttgtggcaa cggcaaggag atacaaatga
    11941 agcaatggat agccgcacta ctgttgatgc ttatacccgg cgtacaggcg gcaaagccgc
    12001 aaaaagtgac gctgatggtg gatgacgttc cggtagctca ggtgttgcag gcgctggctg
    12061 aacaggagaa gttgaacctg gtggtttcgc cagacgtcag cggtacggtg tcgttacatt
    12121 taactgacgt tccctggaag caggcactac aaactgtagt gaaaagcgcc ggattgataa
    12181 cgcgccagga gggcaacatt ctctcggtgc attccgttgc ctggcagaat gacaatatcg
    12241 cccgtcagga ggcggagcag gcgcgggcgc aggcaaatct gccgctggaa aatcgcagta
    12301 taaccctgca atacgccgac gcgggagaac tggcgaaagc cggggagaag ctactgagtg
    12361 ccaaagggag tatgaccgtc gataaacgca ccaatcgcct tttgctgcga gataacaaaa
    12421 cggcgttaag cgcgcttgaa cagtgggtag cgcaaatgga tctgccggtc gggcaggttg
    12481 agctgtcggc gcatattgtc accattaatg aaaaaagttt gcgtgagtta ggcgtgaaat
    12541 ggacgttggc tgatgcgcag caagctggtg gcgttgggca agtcaccacg cttggcagcg
    12601 acctctccgt agcgacggcg acaacgcatg tcggttttaa cattggacgc atcaacggac
    12661 gtttactgga tcttgagctt tccgcgctcg aacaaaaaca gcagctggat attatcgcca
    12721 gtccgcgtct gctggcctca catcttcagc ctgccagcat taaacagggg agcgaaattc
    12781 catatcaggt ttccagcggg gaaagtggcg cgacgtcggt ggaatttaaa gaggccgtcc
    12841 tggggatgga agttacgccc acggtgttac aaaaaggtcg catccggctg aaattacaca
    12901 tcagccagaa cgttccgggg caggtgctac agcaggccga tggcgaagtg ttggcgattg
    12961 ataagcagga gatcgaaacg caggtcgagg tcaaaagcgg agaaacgttg gcgctgggcg
    13021 gcatttttac ccgtaaaaat aaatcgggtc aggatagcgt accgttgctt ggcgacattc
    13081 cctggttcgg gcaattattt cgtcatgacg gaaaagaaga tgaacgacgc gagttagtgg
    13141 tgtttatcac gccacgactg gtttccagtg agtaaacagc cgtaaaagcg gtaatgtttt
    13201 tacgctgaac gtgtttcatc tatttgacgc gcgcaggtat ttagcataca aggagtaccg
    13261 atttgagagt tggtgctctt cgctgcctgc gttccatgat gatgatttat cattcaggcg
    13321 gcattttgct gtctttttta cgctaatctt acccggtgat taatcgccag agcggtggta
    13381 gcaaggcagc gcgcttgcag cgaccagata tgcagaggga tgggtgattt attcagttgc
    13441 caaacccgct ggagtattga gataattttc agtctgactc tcgcaatatc ttatgaggtt
    13501 tcagttcatg tcctgcggcg ctctctgagc gaagcgggtt tatcattaac gaatagtctt
    13561 agtagtaccg aaaaaatggc agagaaacgc aatatctttc tggttgggcc tatgggtgcc
    13621 ggaaaaagca ctattgggcg ccagttagct caacaactca atatggaatt ttacgattcc
    13681 gatcaagaga ttgagaaacg aaccggagct gatgtgggct gggttttcga tttagaaggc
    13741 gaagaaggct tccgcgatcg cgaagaaaag gtcatcaatg agttgaccga gaaacagggt
    13801 attgtgctgg ctactggcgg cggctctgtg aaatcccgtg aaacgcgtaa ccgtctttcc
    13861 gctcgtggcg ttgtcgttta tcttgaaacg accatcgaaa agcaacttgc acgcacgcag
    13921 cgtgataaaa aacgcccgtt gctgcacgtt gaaacaccgc cgcgtgaagt tctggaagcg
    13981 ttggccaatg aacgcaatcc gctgtatgaa gagattgccg acgtgaccat tcgtactgat
    14041 gatcaaagcg ctaaagtggt tgcaaaccag attattcaca tgctggaaag caactaattc
    14101 tggctttata tacactcgtc tgcgggtaca gtaattaagg tggatgtcgc gttatggaga
    14161 ggattgtcgt tactctcggg gaacgtagtt acccaattac catcgcatct ggtttgttta
    14221 atgaaccagc ttcattctta ccgctgaaat cgggcgagca ggtcatgttg gttaccaacg
    14281 aaaccctggc tcccctgtat ctcgataagg tccgcggcgt acttgaacag gcgggtgtta
    14341 acgtcgatag cgttatcctc cctgacggcg agcagtataa aagcctggct gtactcgata
    14401 ccgtctttac ggcgttgtta caaaagccgc atggtcgcga tactacgctg gtggcgcttg
    14461 gcggcggcgt agtgggcgat ctgaccggct ttgcggcggc gagttatcag cgcggtgtac
    14521 gtttcattca agtcccgacg acattgctgt cgcaggtcga ttcctccgtt ggcggcaaaa
    14581 ctgcggtcaa ccatcccctc ggtaaaaaca tgattggcgc gttctaccag cctgcttcag
    14641 tggtggtgga tctcgactgt ctgaaaacgc ttcccccgcg tgagttagcg tcggggctgg
    14701 cagaagtcat caaatacggc attattcttg acggtgcgtt tttcaactgg ctggaagaga
    14761 atctggatgc gttgttgcgt ctggacggtc cggcaatggc gtactgtatt cgccgttgtt
    14821 gtgaactgaa ggcagaagtt gtcgccgccg acgagcgcga aaccgggtta cgtgctttac
    14881 tgaatctggg acacaccttt ggtcatgcca ttgaagctga aatggggtat ggcaattggt
    14941 tacacggtga agcggttgct gcgggtatgg tgatggcggc gcggacgtcg gaacgtctcg
    15001 ggcagtttag ttctgccgaa acgcagcgta ttataaccct gctcacgcgg gctgggttac
    15061 cggtcaatgg gccgcgcgaa atgtctgcgc aggcgtattt accgcatatg ctgcgtgaca
    15121 agaaagtcct tgcgggagag atgcgcttaa ttcttccgtt ggcaattggt aagagtgaag
    15181 ttcgcagcgg cgtttcgcac gagcttgttc ttaacgccat tgccgattgt caatcagcgt
    15241 aacaacaaga aaggtcaggc cgcttatcaa gcggtctatt agcttcaggt taattgcaac
    15301 gtggtaagca ttaacctttt agtggggtgt taaatggatg aattcaaacc agaagacgag
    15361 ctgaaacccg atcccagcga tcgtcgtact ggtcgttctc gtcaatcttc tgaacgttct
    15421 gagcgtactg aacgtggcga accgcagatc aattttgatg atattgaact tgatgacact
    15481 gacgatcgcc gtccgactcg tgcgcaaaaa gagcgcaatg aggaaccgga actcgaagaa
    15541 gaaattgacg aatccgaaga tgaaaccgtg gatgaagagc gcgtagagcg tcgtccacgt
    15601 aagcgcaaaa aagcagccag taaacccgct tctcgtcagt atatgatgat gggcgtcggc
    15661 attctggttc tcctgctgtt gatcatcggt atcggttctg cgctaaaagc cccctcgacc
    15721 tcttccagcg atcaaaccgc gtctggcgag aagagtattg atcttgctgg caatgcgacc
    15781 gatcaggcga atggcgtgca gccagcgccg ggaaccacgt ctgcggaaaa tactcagcag
    15841 gatgtttctc tgccgccgat ctcttctacg ccgactcaag ggcaaacccc ggcggcaacg
    15901 gatggtcaac aacgtgttga agtgcagggt gacctgaaca atgcgctgac ccagccacaa
    15961 aatcagcaac agttgaataa tgtggcggtc aattccacgt tgccgactga acccgcgacg
    16021 gtcgcgcctg ttcgcaatgg caatgcatcg cgtgacacgg cgaaaacgca aaccgctgaa
    16081 cgtccggcca ctacgcgtcc agctcgccag caggcggtga ttgaaccgaa aaaaccgcaa
    16141 gcaaccgtga aaacggagcc gaagccggta gcacagacgc cgaagcgtac tgaaccagct
    16201 gctcctgtgg caagcacgaa ggcaccggct gcgacttcta cgccagcacc aaaagagacg
    16261 gcgactacgg ctccagtaca gacggcatcc ccggcgcaaa ccacggcaac accagccgct
    16321 ggagggaaga ccgcaggtaa tgttggttcg ttgaaatcgg caccgtccag ccattacact
    16381 ctgcagctga gcagttcctc taactacgac aacctgaacg gttgggcgaa gaaagagaat
    16441 ctgaaaaact acgttgtcta tgaaacgacg cgtaatggtc agccgtggta tgtcctggtt
    16501 tctggcgtgt acgcttcgaa agaagaggcg aaaaaagcgg tatctacatt gccagccgat
    16561 gtccaggcca aaaacccgtg ggcgaaaccg ctgcgtcagg tacaggccga tctgaagtaa
    16621 tcaaggttat ctcccgcaat ggtttatcat cgcgggagtt gcctgaagcg ctggatgctg
    16681 tcggagcttt ctccacagcc ggagaaggtg taattagtta gtcagcatga agaaaaatcg
    16741 cgcttttttg aagtgggcag ggggcaagta tcccctgctt gatgatatta aacggcattt
    16801 gcccaagggc gaatgtctgg ttgagccttt tgtaggtgcc gggtcggtgt ttctcaacac
    16861 cgacttttct cgttatatcc ttgccgatat caatagcgac ctgatcagtc tctataacat
    16921 tgtgaagatg cgtactgatg agtacgtaca ggccgcacgc gagctgtttg ttcccgaaac
    16981 aaattgcgcc gaggtttact atcagttccg cgaagagttc aacaaaagcc aggatccgtt
    17041 ccgtcgggcg gtactgtttt tatatttgaa ccgctacggt tacaacggtc tgtgtcgtta
    17101 caatctgcgc ggtgagttta acgtgccgtt tggccgctac aaaaaaccct atttcccgga
    17161 agcagagttg tatcacttcg ctgaaaaagc gcagaatgcc tttttctact gtgagtctta
    17221 cgccgatagc atggcgcgcg cagatgatgc atccgtcgtc tattgcgatc cgccttatgc
    17281 gccgctgtct gcgaccgcca actttacggc gtatcacaca aacagtttta cgcttgaaca
    17341 acaagcgcat ctggcggaga tcgccgaagg tctggttgat cgccatattc cagtgctgat
    17401 ctccaatcac gatacgatgt taacgcgtga gtggtatcag cgcgcaaaat tgcatgtcgt
    17461 caaagttcga cgcagtataa gcagcaacgg cggcacacgt aaaaaggtgg acgaactgct
    17521 ggctttgtac aaaccaggag tcgtttcacc cgcgaaaaaa taattctcaa ggagaagcgg
    17581 atgaaacagt atttgattgc cccctcaatt ctgtcggctg attttgcccg cctgggtgaa
    17641 gataccgcaa aagccctggc agctggcgct gatgtcgtgc attttgacgt catggataac
    17701 cactatgttc ccaatctgac tattgggcca atggtgctga aatccttgcg taactacggc
    17761 attaccgccc ctatcgacgt acacctgatg gtgaaacccg tcgatcgcat tgtgcctgat
    17821 ttcgctgccg ctggtgccag catcattacc tttcatccag aagcctccga gcatgttgac
    17881 cgcacgctgc aactgattaa agaaaatggc tgtaaagcgg gtctggtatt taacccggcg
    17941 acacctctga gttatctgga ttacgtgatg gataagctgg atgtgatcct gctgatgtcc
    18001 gtcaatcctg gtatcggcgg tcagtctttc attcctcaaa cactggataa actgcgcgaa
    18061 gtacgtcgtc gtatcgacga gtctggcttt gacattcgat tggaagtgga cggtggcgtg
    18121 aaggtgaaca acattggcga aatcgctgcg gcgggcgcgg atatgtttgt tgctggttcg
    18181 gcaatcttcg accagccaga ctacaaaaaa gtcattgatg aaatgcgcag tgaactggca
    18241 aaggtaagtc atgaataagt ttgaagatat tcgcggcgtc gcttttgatc tcgacggtac
    18301 gctggttgac agtgcacccg gtcttgctgc tgcggtagat atggcgctgt atgcgctgga
    18361 gttgcctgtc gcaggtgaag aacgcgttat tacctggatt ggtaacggcg cagatattct
    18421 gatggagcgc gcattgacct gggcgcgtca ggaacgtgcg actctgcgta aaacaatggg
    18481 taaaccgccc gttgatgacg acattccggc agaagaacag gtacgtattc tgcgtaaact
    18541 gttcgatcgc tactatggcg aggttgccga agaggggacg tttttgttcc cgcacgttgc
    18601 cgatacgctg ggcgcattgc aggctaaagg cctgccgcta ggcctggtca ccaacaaacc
    18661 gacgccgttc gtcgcgccgc tgctcgaagc cttagatatc gccaaatact tcagcgtggt
    18721 tatcggcggt gatgatgtgc aaaacaaaaa accgcatccg gacccgctgt tactggtggc
    18781 tgagcggatg ggaattgccc cacaacagat gctgtttgtt ggcgactcac gcaatgatat
    18841 tcaggcagca aaagcggcag gttgcccatc agttggctta acctacggat ataactacgg
    18901 cgaggctatc gatctcagcc agcctgatgt aatttatcag tctataaatg accttctgcc
    18961 cgcattaggg cttccgcata gcgaaaatca ggaatcgaaa aatgactaag cccatcgttt
    19021 ttagtggcgc acagccctca ggtgaattga ccattggtaa ctacatgggt gcgctgcgtc
    19081 agtgggtaaa catgcaggat gactatcatt gcatttactg tatcgttgac caacacgcga
    19141 tcaccgtgcg ccaggatgca cagaagctgc gtaaagcgac gctggatacg ctggccttgt
    19201 atctggcttg tggtatcgat cctgagaaaa gcaccatttt tgttcagtcc cacgtgccgg
    19261 aacatgcaca gttaggctgg gcactgaact gctataccta cttcggcgaa ctgagccgca
    19321 tgacgcagtt taaagataaa tctgcgcgtt atgccgagaa catcaatgct ggtctgtttg
    19381 actatccggt gctgatggct gcggacatcc tgctgtatca aactaatctg gtaccggtcg
    19441 gtgaagacca gaaacagcac ctggaactga gccgcgatat cgcccagcgt ttcaacgcgc
    19501 tgtatggcga gatctttaaa gtgccggagc cgtttattcc gaaatctggc gcgcgcgtaa
    19561 tgtcgctgct ggagccgacc aaaaagatgt ccaagtctga cgataaccgc aataacgtta
    19621 tcggcctgct ggaagatccg aaatcggtag tgaagaaaat caaacgtgcg gtcactgact
    19681 ccgacgagcc gccggtagtt cgctacgatg tgcagaacaa agcgggcgtt tccaacctgc
    19741 tggatatcct ttctgcggta acgggccaga gcatcccgga actggaaaaa cagttcgaag
    19801 gcaagatgta tggtcatctg aaaggcgaag tggctgatgc cgtttccggt atgctgactg
    19861 aattgcagga acgctatcac cgtttccgca atgacgaagc cttcctgcaa caggtgatga
    19921 aagatggcgc ggaaaaagcc agtgcacacg cttctcgtac gctgaaagcg gtgtacgaag
    19981 cgattggttt tgtggcgaag ccgtaattca ctattgctgg caaattgcgc tttgttcatg
    20041 ccggatgcgg cgtgaacgcc ttatccggcc tacaaagtaa tgcacatatt gcaaggaaat
    20101 gtaggcctga taagcgtagc gcatcaggca attttgcttt tatcatcact ctgaaaccgg
    20161 ggaaacccgg tttttttatc ctcatttgca ataatccgaa aaaatgtgaa gtgcctcgcc
    20221 gtttccacat ctcgctgttg cgccttatct tcaaattcct gaagatataa atattcaatt
    20281 tactgaaaat ttattatgcg tcgaacgttt atcaaaaaag aaggcgtcgt catcacgaca
    20341 ctggcccgtt atttgttggg tgaaaagtgc ggtaatcgac tgaaaaccat agatgaactg
    20401 gcaactgaat gccgttcatc cgttggcctg acgcaggccg cgttgaaaac gctggaatca
    20461 agcggagcga tacggataga acgccgtggg cgtaatggca gttatctggt ggaaatggat
    20521 aacaaagcat tgctgagtca tgtggatatc aacaatgtgg tgtgcgcgat gcccttgccc
    20581 tatacccgtt tgtacgaagg cctggcgagc ggattgaaag ctcagtttga tggtattcct
    20641 ttttactatg cgcacatgcg cggcgcggat attcgcgtgg agtgtttact taacggcgtg
    20701 tatgacatgg cggttgtttc ccgactggcg gcggaaagtt atctcacgca aaaagggtta
    20761 tgcctcgcgc tggagttggg gccgcacacc tacgttggcg agcaccagtt gatttgccgt
    20821 aaaggcgagt ccgcaaacgt gaatcgcgtg gggctggata accgttcggc ggatcagaaa
    20881 atcatgaccg atgtcttttt tggcgatagt gatgtggaac gagtcgatct ctcttatcac
    20941 gagagtttac aacgcattgt taaaggcgat gttgatgcgg tgatctggaa cgtggtggcg
    21001 gaaaacgaac tgaccatgct gggattagaa gcgacgccgc tcacagacga tccgcgattt
    21061 ttacaggcca ccgaagccgt agttttgacg cgagtcgatg attacccaat gcaacaattg
    21121 ctgcgtgcag ttgttgataa acaggccctg cttgcccatc aacaacgagt ggtcagtggg
    21181 gaacaggaac cgagttatta aacgaaaggc atctgaaatg gaaaccagac tcaacctgct
    21241 ttgtgaggca ggcgttattg ataaggacat ctgcaaaggc atgatgcagg tcgtcaacgt
    21301 actggaaaca gagtgtcatc taccggtgcg cagcgagcaa ggaatgatgg cgatgacaca
    21361 tatggcgagc gcactgatgc gcagtcgccg tggtgaagaa atagagccgc tggatgacga
    21421 attgctggca gagctggcgc aatccagcca ctggcaagcc gttgtgcaat tgcatcaggt
    21481 gttgttgaag gaattcgcac tggaagttaa gccgtgtgaa gaaggctatt tgctggctaa
    21541 tctttatgga ttatggatgg ctgctaacga agaggtttga agctcccgac aaatccacgt
    21601 cgcataatct aaatattcag aaaaatgaat atttaattca aggaacagaa acgaggcaac
    21661 aagatgtttg tagaagcatt gaaacgccag aacccggcgc tgatttccgc cgcactaagt
    21721 ttgtggcagc agggcaagat cgccccggac agctgggtga tcgatgtgga tcagatactg
    21781 gaaaacggta agcggctgat tgagacggcg cgactttacg gcattgaact gtatctgatg
    21841 accaagcagt ttggccgtaa tccgtggctg gcggaaaaac ttctggcact gggctacagc
    21901 ggcattgtgg cggtggatta caaagaggcg cgggtcatgc gccgcgccgg tctgccgttg
    21961 gcgcatcagg ggcatctggt gcaaatccct tgtcaccagg tttctgacgc cgttgcacag
    22021 ggaactgacg tcatcaccgt gtttactctc gataaagcgc gggaagtttc tgcagcagcg
    22081 gtgaaggccg gacgtgtgca gtctgtgctg ctcaaagttt atagcgacga tgattttctt
    22141 tatccggggc aggagagcgg ttttgtacag cattcgctgc acgaggtggt tgctgatatt
    22201 aagaaactgc cggggctgca tttagccgga ctaacccatt tcccttgcct gctttgggat
    22261 gaggctgccg ggaaagtttt gccgacaccg aatcttcaca cgctgataca ggcacgggat
    22321 caactggcga aatctggtat tgcgattgag caactgaacg caccttctgc gaccagctgc
    22381 acttcgctgc cattgctggc ggaatacggt gtgactcatg ccgaacccgg tcatgcgctg
    22441 acggggacga ttccggcaaa ccagcagggc gatcagcctg aacgtatcgc gatgctctgg
    22501 ttaagtgaaa tctcccatca ttttcgtggc gacagctact gctacggcgg cggttactac
    22561 cgtcgtggtc acgcgcaaca tgcgctggtg tttacgccag aaaatcaaaa gattactgaa
    22621 accaatctca aaactgtgga tgacagcagt atcgactaca ccctgccgct ggcaggcgag
    22681 tttccggtaa gcagtgcagt agtgctctgt tttcgcacgc agatttttgt cacccgtagc
    22741 gatgtggtgt tgctgtccgg tattcaccgt ggcgaaccag aaatcgtcgg tcgttatgac
    22801 agtcttggaa actctctggg ggcgtaatgg cgcgatttgt ggtgttagtg attgatagct
    22861 ttggcgtagg ggcaatgaaa gatgtcacgt tggtgcgtcc gcaggatgcc ggggcaaata
    22921 cctgtggtca catcctgagc cagttgccgc atttgcagct accaatgctg gagaaaatgg
    22981 ggctaatcaa cgcattaggt tatgcgcctg gagatatgca gccgtcagac tccgcaacct
    23041 ggggcgtggc agagctgcaa catgaaggtg gcgatacctt tatggggcat caggaaattt
    23101 taggcacgcg cccgttaccg ccgctacgga tgccctttcg cgatgtgatt gaccgtgttg
    23161 agcaagcatt acttaacgct gactggcagg tggagcgccg tggcgatgat ctgcaatttc
    23221 tgtgggtcaa tcaggcggtt gcgattggcg ataatctcga ggcggattta ggtcaggtct
    23281 ataacatcac cgccaatctc tctgtgatct cttttgacga cgcaatcaaa attggtcgta
    23341 tcgtgcgtga gcaggtacag gtcggtcggg tcattacatt tggtggcctg ttaaccgaca
    23401 gccagcgcat cctcgatgcc gcagaaagca aagaagggcg ctttattggc atcaatgcgc
    23461 cgcgttctgg cgcttatgac aacggcttcc aggtcgtgca tatgggctac ggcgtcgatg
    23521 aaaaagtgca ggtgccacaa aaactgtatg aagtgggcgt accaaccgtg ctggtgggga
    23581 aagtggcgga tattgccagt aatccttatg gcgtgagctg gcaaaacctg gtggatagcc
    23641 agcggattat ggatatcacc ttcaacgaat ttaacaccca tccgacggcg tttatttgta
    23701 ccaatatcca ggaaaccgac ctcgctggtc atgcagaaga cgtcgcacgt tatgcggaac
    23761 gtttgcaggt cgttgaccgt aaccttgccc ggcttattga ggcgatgcag ccagatgatt
    23821 gcctggtcgt gatggcggat cacggtaacg atccgaccat tggtcacagc caccataccc
    23881 gcgaagtggt gccagtgctg gtttatcagc aagggatggt cgctacgcag cttggtgtgc
    23941 tcaccacgct ttctgatgtg ggggctaccg tatgtgaatt tttccgcgcg ccaccgccac
    24001 aaaatggtcg ctcttttctt tcctccctcc ggtttgcagg agacacccta tgagttttga
    24061 tccgacgggc tacaccctcg cccatgagca tctgcatatt gatctctccg gctttaaaaa
    24121 caacgtggac tgccgccttg atcagtatgc gttcatttgc caggagatga acgacctgat
    24181 ggcccagggc gtgcgtaatg tgattgagat gaccaaccgt tacatggggc gcaatgcgca
    24241 atttatgctc gatgtaatgc gcgaaaccgg gatcaacgtg gtggcctgta ccggttatta
    24301 ccaggacgcg tttttcccgg aacatgtggc gacccgcagc gtgcaggaac tggcgcaaga
    24361 gatggtcgat gaaattgaac agggtatcga tggcacggag ctgaaagccg ggatcatcgc
    24421 ggagataggc tccagcgaag gaaaaatcac gccgctggaa gagaaggtat ttattgccgc
    24481 cgcgctggcg cataaccaga ccggacgccc gatctccacg catacctctt ttagcaccat
    24541 ggggctggaa caactggcgc tactgcaagc ccacggagtt gatctttcgc gcgtcaccgt
    24601 tggtcactgc gatctgaaag acaacctcga caacattgtg aagatgatcg atctcggcgc
    24661 gtatgtgcag ttcgacacca tcggtaagaa cagttactac ccggacgaaa agcgcattgc
    24721 gatgctccac gcgctacgtg accgaggttt gcttaaccgc gtcatgctgt cgatggatat
    24781 tacgcgccgc tcccatttaa aagccaacgg tggttatggc tatgactatt tattgaccac
    24841 ctttattccg caattgcgcc agtcaggatt cagtcaggcc gatgtggatg tgatgttacg
    24901 tgaaactccc tctcaatttt tccaataagg acagactcat gaaaaagatt ggtgttgcag
    24961 gcttacagcg tgagcagatt aaaaaaacca ttgaagcgac ggctcctggc tgttttgaag
    25021 ttttcattca caacgacatg gaagcggcaa tgaaagtcaa atccgggcaa ctggattact
    25081 acatcggcgc gtgtaatacc ggtgcgggcg cggcattgtc gattgccatc gcggtgattg
    25141 gctataacaa aagttgcacc attgccaaac caggcattaa agcgaaagac gaacatatcg
    25201 ccaaaatgat cgccgaaggg aaagtggcgt ttgggctttc cgttgagcac gtcgaacacg
    25261 cgattccgat gctgattaac catctgaaat aaaaggcacg actatggatc tgtatattca
    25321 gattatcgtg gtggcgtgcc tgacgggtat gacatcgctt ctggcgcatc gctcggcggc
    25381 cgtttttcat gacggtatcc gcccgatcct gccgcaactg attgaaggct atatgaaccg
    25441 tcgcgaggcg gggagtatcg cttttggtct gagcattggt tttgtggctt cggtggggat
    25501 ctcttttacc ctgaaaaccg gactgctcaa cgcatggtta ctctttcttc ctaccgatat
    25561 cctcggcgta ctggcgataa acagcctgat ggcgtttggt cttggcgcta tctggggcgt
    25621 gttgatcctt acttgcctgt taccggtaaa ccagctgctg accgcgctac cggtggatgt
    25681 attaggtagc ctgggggaat taagctcgcc ggtggtttca gcttttgcac tgttcccgtt
    25741 ggtggcgatt ttctaccagt ttggctggaa gcaaagtctg atcgccgccg tggtggtact
    25801 gatgacccgt gtggtagtag tgcgctattt cccgcatctc aatccggaat ccatcgaaat
    25861 ctttattggc atggtgatgc tgctggggat cgcgattact cacgacctgc gtcatcgtga
    25921 tgaaaatgac attgatgcca gcgggctttc ggtgtttgaa gaacgcacgt cacggattat
    25981 caaaaactta ccgtatatcg ctattgtcgg ggcgctgatt gccgccgttg ccagcatgaa
    26041 gatttttgct ggcagtgaag tgtcgatctt cacactggag aaagcatatt ccgcaggcgt
    26101 aacgccggaa caatcgcaaa cactaatcaa ccaggcagct ctggcggaat ttatgcgcgg
    26161 gctgggtttt gtaccgttga ttgccaccac cgcgttagcc actggtgtgt atgcagttgc
    26221 gggctttacc tttgtttatg cggtgggcta tctctcgccg aatccgatgg ttgcagcggt
    26281 attaggcgca gtggttattt cggcggaagt cttgctgctt cgttcgatcg gcaaatggtt
    26341 ggggcgctac ccgtcggtac gtaatgcgtc ggataacatc cgtaacgcca tgaatatgct
    26401 gatggaagtg gcgttgctgg tcggttcgat ctttgcagca atcaaaatgg cgggctatac
    26461 cggattctct atcgcggttg ccatttactt cctcaacgaa tccctgggcc gtccggtaca
    26521 gaaaatggcg gcaccggtcg tagcagtaat gatcaccggc atcctgctga atgttcttta
    26581 ctggcttggc ctgttcgttc cggcttaagg aaggcaccta tgaagacgtt tcctctgcaa
    26641 agcctgacgc ttattgaggc gcagcaaaag cagtttgcgc tggtggacac gatttgccgt
    26701 cattttcctg gcagcgagtt tcttacctgc ggtgatttgg gcttaacgcc ggggctaaat
    26761 cagccccgca ttacccagcg tgtggaacag gtgctggctg acgcgtttca tgcacccgcg
    26821 gcggcactgg tacagggcgc gggtactggc gcgattcgtg cggcactggc ggctttgctc
    26881 aaaccggggc aacgtcttct ggtgcatgat gcgccggttt atccaacgac gcgcgtcatt
    26941 attgagcaga tggggctaac gctcattacg gctgatttca atgatctgtc ggcactgaag
    27001 caggttgttg acgagcaaca acctgatgcc gcgctggtgc agcatacgcg ccagcagccg
    27061 caggaccgct atgtgctggc agacgtgctg gcaacgttgc gcgcggcagg tgttccagcg
    27121 ttaaccgatg acaactatgc ggtgatgaag gtgtcgcgaa ttggctgcga atgcggggcg
    27181 aatgtgtcga cattttcctg cttcaagcta tttgggccag agggcgttgg tgcggtggta
    27241 ggcgatgccg atgttattag ccggattcgc gccacgctct attccggcgg cagtcagatt
    27301 cagggggcgc aggcattaga ggtgttgcgt ggtctggtgc tcgcgccagt gatgcacgcg
    27361 gtgcaggcgg gggtatctga acggttgctg gctttactta acggtggcgc agtggcggaa
    27421 gtgaaaagcg cggtgattgc caatgcgcag tcgaaggtgt tgattgtgga gtttcatcag
    27481 ccgattgccg ccagagtact ggaagaggcg caaaagcgcg gtgccttgcc ttacccggtt
    27541 ggtgcagagt cgaaatatga aattccgcca cttttttatc gactttccgg aacgtttcgc
    27601 caggcgaatc cgcaactcga acattacgcg attcgtatta acccaaatcg cagcggtgaa
    27661 gagacggtgc tgcggatttt gcgtgagagt attgccagta tttaatcttg tgcatgatgt
    27721 tttcgaaaag aatgcagaaa taaatacgcc ctcgggtaac cccgagggcg tcaggcaatt
    27781 aaaccggtgt tagccgcttt cagtcagaga cttactgtgc agtaggaccg caaccgccaa
    27841 tgattttaga aatagaaatt gccgggtgca gcaggtagtc gtaagagcag ttcttatcac
    27901 ggttttcaat atgaccagta catgcagtta acgtagccag taccgcacct accagtgcaa
    27961 ctttaataaa tttgttcatt
//
LOCUS       DAZGAP010000051        27785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGAP010000051 DAZGAP010000000
VERSION     DAZGAP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.051"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..294
                     /gene="yhcN"
                     /locus_tag="ACHLAM_001825"
     CDS             31..294
                     /gene="yhcN"
                     /locus_tag="ACHLAM_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO7853359.1"
                     /translation="MKIKTTVTALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(349..621)
                     /gene="yhcO"
                     /locus_tag="ACHLAM_001826"
     CDS             complement(349..621)
                     /gene="yhcO"
                     /locus_tag="ACHLAM_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO7853360.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(713..2680)
                     /gene="aaeB"
                     /locus_tag="ACHLAM_001827"
     CDS             complement(713..2680)
                     /gene="aaeB"
                     /locus_tag="ACHLAM_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO7853361.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(2686..3618)
                     /gene="aaeA"
                     /locus_tag="ACHLAM_001828"
     CDS             complement(2686..3618)
                     /gene="aaeA"
                     /locus_tag="ACHLAM_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO7853362.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3626..3829)
                     /gene="aaeX"
                     /locus_tag="ACHLAM_001829"
     CDS             complement(3626..3829)
                     /gene="aaeX"
                     /locus_tag="ACHLAM_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO7853363.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4012..4941
                     /gene="aaeR"
                     /locus_tag="ACHLAM_001830"
     CDS             4012..4941
                     /gene="aaeR"
                     /locus_tag="ACHLAM_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO7853364.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5069..6514)
                     /gene="tldD"
                     /locus_tag="ACHLAM_001831"
     CDS             complement(5069..6514)
                     /gene="tldD"
                     /locus_tag="ACHLAM_001831"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO7853365.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6670..10470)
                     /gene="yhdP"
                     /locus_tag="ACHLAM_001832"
     CDS             complement(6670..10470)
                     /gene="yhdP"
                     /locus_tag="ACHLAM_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO7853366.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10538..12007)
                     /gene="rng"
                     /locus_tag="ACHLAM_001833"
     CDS             complement(10538..12007)
                     /gene="rng"
                     /locus_tag="ACHLAM_001833"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO7853367.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(11997..12590)
                     /gene="yhdE"
                     /locus_tag="ACHLAM_001834"
     CDS             complement(11997..12590)
                     /gene="yhdE"
                     /locus_tag="ACHLAM_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO7853368.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12599..13087)
                     /gene="mreD"
                     /locus_tag="ACHLAM_001835"
     CDS             complement(12599..13087)
                     /gene="mreD"
                     /locus_tag="ACHLAM_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO7853369.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13087..14190)
                     /gene="mreC"
                     /locus_tag="ACHLAM_001836"
     CDS             complement(13087..14190)
                     /gene="mreC"
                     /locus_tag="ACHLAM_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO7853370.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14256..15299)
                     /gene="mreB"
                     /locus_tag="ACHLAM_001837"
     CDS             complement(14256..15299)
                     /gene="mreB"
                     /locus_tag="ACHLAM_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO7853371.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15604..17544)
                     /gene="csrD"
                     /locus_tag="ACHLAM_001838"
     CDS             complement(15604..17544)
                     /gene="csrD"
                     /locus_tag="ACHLAM_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO7853372.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17696..18670
                     /gene="yhdH"
                     /locus_tag="ACHLAM_001839"
     CDS             17696..18670
                     /gene="yhdH"
                     /locus_tag="ACHLAM_001839"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO7853373.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18856..19243
                     /locus_tag="ACHLAM_001840"
                     /pseudo
     CDS             18856..19243
                     /locus_tag="ACHLAM_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            19648..20118
                     /gene="accB"
                     /locus_tag="ACHLAM_001841"
     CDS             19648..20118
                     /gene="accB"
                     /locus_tag="ACHLAM_001841"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO7853374.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20129..21478
                     /gene="accC"
                     /locus_tag="ACHLAM_001842"
     CDS             20129..21478
                     /gene="accC"
                     /locus_tag="ACHLAM_001842"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO7853375.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            21587..21829
                     /gene="yhdT"
                     /locus_tag="ACHLAM_001843"
     CDS             21587..21829
                     /gene="yhdT"
                     /locus_tag="ACHLAM_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO7853376.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            21819..23270
                     /gene="panF"
                     /locus_tag="ACHLAM_001844"
     CDS             21819..23270
                     /gene="panF"
                     /locus_tag="ACHLAM_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO7853377.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRTVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            23282..24163
                     /gene="prmA"
                     /locus_tag="ACHLAM_001845"
     CDS             23282..24163
                     /gene="prmA"
                     /locus_tag="ACHLAM_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO7853378.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            24492..25457
                     /gene="dusB"
                     /locus_tag="ACHLAM_001846"
     CDS             24492..25457
                     /gene="dusB"
                     /locus_tag="ACHLAM_001846"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO7853379.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            25483..25779
                     /gene="fis"
                     /locus_tag="ACHLAM_001847"
     CDS             25483..25779
                     /gene="fis"
                     /locus_tag="ACHLAM_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO7853380.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            25865..26749
                     /gene="yhdJ"
                     /locus_tag="ACHLAM_001848"
     CDS             25865..26749
                     /gene="yhdJ"
                     /locus_tag="ACHLAM_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7853381.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAVLVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVANGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            26833..27012
                     /gene="yhdU"
                     /locus_tag="ACHLAM_001849"
     CDS             26833..27012
                     /gene="yhdU"
                     /locus_tag="ACHLAM_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO7853382.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(27015..27677)
                     /gene="acrS"
                     /locus_tag="ACHLAM_001850"
     CDS             complement(27015..27677)
                     /gene="acrS"
                     /locus_tag="ACHLAM_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO7853383.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT         6775 a         7382 c         6954 g         6674 t
ORIGIN      
        1 cagctaaaag attatgtcga ggtaaaaatc atgaaaatca aaaccactgt tactgcatta
       61 agcgtacttt ctgttctctc tttcggtgca ttcgctgccg actccattga tgctgcacaa
      121 gcacaaaatc gtgaagcaat cgggaccgta tccgtaagtg gtgtggcgtc ttcgccaatg
      181 gatatgcgtg aaatgctgaa caaaaaagcg gaagagaaag gcgcaacggc ctaccagatt
      241 actgaagctc gtagcggtga cacctggcac gctacggctg aactgtacaa ataaaccctc
      301 atcgtcttgt ccgacgatat tgcccccggt tcgggggctt tttttgcgct aatgacgaac
      361 attaaaacgc aaatgccctt ccagctcttc ctctgcctca tcaaacagca atattaacgc
      421 gccaaaacgg cgacgcgttt tctctcccag atgaacaaat tcaatctcaa gtggtagcgg
      481 caggacatcg ttcattaaca catcccatag tgagtcgaga tcgcgtacct tatctttcgc
      541 cagaccaaag gcttggctga agtcgcgata aaaatcctcc tgactctcaa tctcatcaaa
      601 atcaaaggta taaatattca tctgttgcca ccgtcacgtt tcacggcgag ttctgccgtt
      661 catcgctaag tatagccatg aaaaaaccga cgcttcgggc gtcggttttg acttaactat
      721 cggtcaacgc atgttgatac ttatggagca tccccgccag ccgatgaacc ggttccgtca
      781 cctgcggtgg cgcttgccag atgcgtagct tttcctggta gatttcaagt tcttccagca
      841 actggccaaa gtaccgacga cgtttatcat cgctacgggc agatatcaca tggtctgctg
      901 tgcggcgcat ttgtcggtga aacgccgata aatcctcgtt aaccgggatc ggcgcatcac
      961 gcaggcgctg gtgcgcgata atcatcgtca gcgccaggcg aaatttcggc aaatcccctg
     1021 ggaacttatt catcagcaaa aacagctgct gataaagtgc cgggaggtga ttctctttac
     1081 gacgtgccac attggtagtc atcgcggaaa cagcggcaga aacaaactga ttaagcagta
     1141 cacgtccagt cctgtcgcgc gatttatccc gcaccagcaa aataacggtg aacgcgagca
     1201 cacagccgac gatttgccct aatgcgctgt cgagaaactg actgaaatgg aaagtcatcg
     1261 ggttatccag cacgataata tttatggtgc tggccagcgc ccccatcgag cccagtcgcc
     1321 gtttctgtac ttctataccg aggaaaaatc ccagcactgc caggctaata cacagcaata
     1381 acatgctttg ttgggtatta gggataatca ccaaaaagta gagcagccct aacggcagcg
     1441 ccgccagcgt cccatagata aagtcgatcg ccaccatgcg tggattcggc aaacgcattg
     1501 ccagtgacgt cacgaccgca atcatcacca ttgcaccact gccggaagtc cagcccgtcc
     1561 acagccagaa aagcgtcccc agaatgcagg aaagtgtggt tcgccagaag ttaaccatcg
     1621 catgatgacg ttcggctgac tctactttga cttccggttc gccttgcagg atctcttctt
     1681 cagtagcgtt gatttttgtg ttactgataa cgccgcgctt gagaagctga taacgcgttg
     1741 ccgccgcgac ccagctataa atggtgacag gcgtttcccg ttccccggtc caggcgataa
     1801 ctctccgcag gcgtttgagc tgcttgtgga cgtcctgcgc ggtttctacc ggcgtgtcaa
     1861 aaaattcgcg gaaagtatca gtgatcaatt ccgggcgcgt attctgaata agataagttt
     1921 cgcaggattg ggtaatcagc gtcagcgata gcgtattgat cgcttttaaa cgtcgattgg
     1981 cccgcgccca gcgggaagat tccatattca ggttgctgcg cataccttgt aacgccgtgg
     2041 tgcgtcgcac cagatcgccc caggctttat cgacaacttc accatcgcca tgtttgatac
     2101 agagttgcat taattgatat tgcgcgacca gcaaactttc cagctctcga tccacttctt
     2161 gtttgatcga tcgcggagaa aagagcaaat ccgccataat cgcacacaca ataccgataa
     2221 cgatctcgct acaacgttcg acggcaaact gcggcgtaag caatggttcc ggctgaatgg
     2281 tgatcacaat gatcagcgcg gtataaccgg ccagccccca cgcatacgag ttttctattc
     2341 gtaccagcga ggatatccag gtacaaaaac cggcccagat acagcacacc agaatcatca
     2401 ataatggtgc gcggatcatc gcaatgatga ttaccagtcc agcaatacag ccaataaatg
     2461 tgccgatgat gcgcaaaaag ccacgatagc gaatagcgcc agaatacggt tcacctcccg
     2521 cagcaaaggc cggaccggcg gcaacaatcg ccgctgtcag taccgcccag cgtggcgttt
     2581 ccagctggaa gtgaaagcca acaaacagcg ccagtacaat ggcggtcgcc agttttaccg
     2641 caaagcgaat atgttgatta gcaatggaga aaatacccat cgtgattaac caaactcacg
     2701 caggcgatgg gccattttac ggaagaacga atcctggctt tcgtcgcgat cttgtttgcc
     2761 agtgaccacc actgtagcag tggtgcccgc aggccagatg ttctcttgct ggttgtcgag
     2821 acgaatacgt accggaacac gttgcgccaa acgtacccat tcaaggttag agtcgatggt
     2881 cgccatccct ttgtcgtcac gcgtgctgct ggcgttggtg acccctgcgg caacactatc
     2941 aacagtccct ttcagcactt tgttactgcc aagcggcgtg atctccgcac gatacccagg
     3001 gcgcacccct tccagcttag tttcttccat ataggccagt acatagaagg agttctgttt
     3061 caccagcgca accgccgttg atcctcgagt aataaactca ccggtataga cgttgaggtt
     3121 ggtcacccag ccatctgctg gcgcgcggat gactgtacgt tcaagatcca gttttgccag
     3181 atcacgggtc gcctgcgctt tcgctaactg atgcagaacc gtttgtagta cgttgttggc
     3241 ctgatcgatc tcttcgcgag acatcgcctg cacaccgaga cggttacgac gtcctgcctc
     3301 ctggcgtttc tcctgtgcca gtacctgata ataagcaaca tcggcttgcg cttcctcaag
     3361 cgccttttga tagcgcggct ggtcgatggt gaacagtacc tgtccttttt tcaccagctg
     3421 gttatcatga acattcacct gggtaatgag tccagaaacg tccggcgcga tcgcaacgac
     3481 gtcagcgcta aagcgcgcgt cacgcgtcca cggggattcg gtgtaataga cccaggcatt
     3541 aaaaattgcg atgaaggcca gaatgactaa tacgaccgtg atggccgtac gggagaattt
     3601 tcttattagt gttttcactt caacctcaaa cgaacagtcg cgatatcaaa taaaacaagc
     3661 agcaatagag cgcggtgttg aacaacgccg gatgccagac aaagtcgtag atacctgttg
     3721 gcacaagtac ccggcgcacc agccagaaaa tcgccagtga taaaagcaat tcaaaaaata
     3781 tcggtgggaa ggacagccca aacaccacga taacgggaaa cagactcatg ttgaccttgg
     3841 ttgtatagag agagcaggcg ttattatttt cagcatctgt cgccgcagag aagggcatgg
     3901 aaagccgggc gagagcaaca ttgctgtaga ttgatattta atatattagc gtaactgtta
     3961 tgctgttatc tatattatgt gatctaaatc acttttaagt cagagtgaat aatggaacga
     4021 ctaaaacgca tgtcggtgtt tgccaaagta gttgaatttg gctcttttac cgccgccgcc
     4081 agacagctac agatgagcgt ttcgtccatc agtcagacgg tgtcaaaact ggaagatgag
     4141 ttacaggtaa agctgttaaa ccgtagtaca cgcagcattg gcctgactga agccggcaga
     4201 atttactacc agggctgccg tcggatgctc catgaagtgc aggatgttca tgagcaactg
     4261 tatgccttca ataacacccc catcgggacg ctgcgcattg gctgttcttc aactatggca
     4321 caaaatgttc tcgccgggct gacagcaaaa atgctgaaag aatacccagg tttgagcgtc
     4381 aatctggtta ccggaattcc tgcccccgac ctgattgccg atggtctgga tgtggtgatc
     4441 cgcgtcggcg cgttgcagga ttccagcctg ttttcccgcc gtctgggcgc gatgccgatg
     4501 gtggtgtgcg ccgcgaaaag ctatctcaca caatacggca taccggaaaa acccgccgat
     4561 ttgagtagtc attcatggct tgaatacagc gtgcggcccg acaatgaatt tgaactgatc
     4621 gcaccggaag ggatctcgac tcgcctgatc ccacaaggaa gatttgtgac taatgatccg
     4681 atgacgctgg tgcgctggct gacggcgggt gccgggatcg cctacgtgcc gctgatgtgg
     4741 gtgatcaacg agatcaatcg tggggaactg gagatcctgc tgccgcgtta tcagtcagat
     4801 ccacgcccgg tttatgcgtt atataccgaa aaagataagc tgccgctgaa ggtacaggtc
     4861 gtgatcaact cgctgacgga ttattttgtt gaggtcggta aattgtttca ggagatgcac
     4921 gggcgcggga aagagaagta attcatattg tactgttacg ttgtacaaac ctgtgccaac
     4981 gggttcccct caccctaacc ccagaggagc gaggggaccg ttcgtgcacg tagaaagatt
     5041 aattatcctt ctgaaaatac tgaaattatt acgcagtacc gccaacagtc aggttatcga
     5101 ctttcaacgt tggctggccc acgccaaccg gcaaactttg cccttcttta ccgcagacac
     5161 ccacgccgtt atccagtttc aggtcgttac ccaccatcga aatctgctgc atggtttcga
     5221 taccggaacc aatcaacgtt gcgcctttca ccggcttcgt tactttaccg ttttcaatca
     5281 gatatgcttc tgaagtggag aaaacgaatt tgccggaggt gatatccacc tgaccgccac
     5341 caaagttcgg tgcatagata ccgtactcaa cggattcaat aatttcctgc ggggtcgatt
     5401 tacccggcag catataggtg ttggtcatac gcggcatggg cagatgggcg taggattcac
     5461 ggcgaccgtt gccagtcggc gtcatcccca tcaaacgcgc gttgagttta tcctgcatgt
     5521 agcctttcag gatgccattc tcaatcagca cgttgtactg gcctggcgta ccttcgtcat
     5581 caatcgccac cgaaccacgg cgatcgacca tcgtaccgtc atcaaccacg gtacacagtt
     5641 ctgaagccac cagctcccca acctgtccac taaatactga agtgccacgg cggttgaagt
     5701 cgccttccag accgtgacct accgcttcat gcaacagcac gcccggccaa cctgcgccaa
     5761 gtactaccgg catggtgccc gctggtgcag caacggcaga aagattgacc agcgccatac
     5821 gcactgcttc ttttgcccat gcatccgcac ggacttcgcc gtcgagatcg gcaaggaaga
     5881 actcataacc aaaacgaccg ccgccgccac tggcaccgcg ttcgcgtttg ccatcttctt
     5941 cgaccagaac gctcacggaa agacgcacca gcggacggac atccgccgct agcgtgccgt
     6001 cggtggccgc aaccagaatt aattcataga caccactgag gctggcagtc acttcctgta
     6061 cgcgcttgtc cgcttcgcgg gcaaccttat cgacgcgacg caggatatcc agcttctctt
     6121 cacggctcat gctttgcagc ggatctaccg aggtatacaa cgggctatgc tctaccgcgc
     6181 ccagcgtctg tactttacca tcaccactat cacggacgat ggtgcgcgcc gcttgtgcac
     6241 tctgttccag cgccagcagg ctgatttggt cagcataagc aaatccggtt ttttcaccac
     6301 tgattgcacg cacgccaacg ccctgatcga tgttgtaaga accatcttta ataatacggt
     6361 cttctaaaac ccaggattcg tgatagctcg actgaaaata gagatcgcca taatcaaggc
     6421 gacgttcggc cagttgaccg aggatcgcga acaagtcctg atgtttcagg ccgttcgccg
     6481 ctagcaattg ttcacttacc aggttcagac tcatcgtttt tgctactcgt tagttactgc
     6541 agtagaggat tttttacggc tgccggatgc ggcgtgaacg ccctgtccgg cctacggtta
     6601 tgttctggct cgttggcctt tggcaacgat tatcctatga gattggggca attacgcgcc
     6661 ctcgtcaaat cattgcgctt tttctttacg cggttgtcgc aacacttcgt tgatttgcgg
     6721 atcgtccagc ggacccgaaa tgtgatagcg caaaatggag actttgctcc acagcggtcc
     6781 cagcacttta ctggcggcaa acactgccgc gccaacaatg gggttaaccg caaaggccgc
     6841 agccacaccc accgtcgcag aaatctctgg tgcgacaacc gcttccatat tcaggtcgcg
     6901 acgtaccaga tttaccgacc ctttcatagc gatgtccgct tccaggccat ccaccagcgt
     6961 gtcgtcggtg tgcataacgc cgtctttaat ccacgcggtg ctgcgaatgg agtcaaaata
     7021 gaacccttcg ccaaaagtgt ctctgaaatc aaaacgcagc ttacgcatca gggcatctac
     7081 gctcaataag cgcagcaatt gcccggcatg tccggtattg atttcggtaa tttcgccttt
     7141 acccagttga gtatgaatga tgccattcaa cgtcgcctca tctggttgcc agggtgcttt
     7201 gcgccagtgt aaatcgtaat ccacattaaa tgacgactgg cgtatgggcg tcgtgacacc
     7261 aaaaaattct gcggcggcat caattttctg cccacgcagt tttcctttca gcgaggtgcg
     7321 ttcattcccc ggattgttaa cccattcacc atcggcagtg agccgcgaaa aaccagtatc
     7381 aatcagtcca ttagtcagcg ttaatgtatt gccagaaatg gtgatatcac tgtcaatgcg
     7441 cccgaatttt tgcccccaga accagcactc tgcgcatcgt atttgtgcgt ccggccagcc
     7501 acggaagcta atgcgctccg ttgtcgggaa aggtgatgaa ttcgttgagt caccatgagt
     7561 tttcgccacg cttgggttgt aataaagata tttgatattc gccagccacg gcgcgttatt
     7621 acgcatcgcc agcgtggcgt tgatttcacg cccttgcgcc tcaaccaggg tgccatttgc
     7681 cgtcggttgc gaaacaatgc tcaggttatt ccattgctga tttcccagcg acaacatagg
     7741 cgtacgtaac gttatgtgtt gtgggaaact cgctgcacca ccgacactct ccgcagcgcc
     7801 tttctggaac agggccagcc actcggcacc attcatcggc ggcatattga gttcaacgcc
     7861 actttgttcc ggcaacggcg ggagcgtttt actgtctgcc gcccaaatag cacggtcgag
     7921 cgtcagcttt tgaccgagca accagcggct attgaaatgg ttatctgcac cagcctgtcc
     7981 ggttaactca aagctgttga gattgccatc aaccttaacg tttaccggca gtggttcacc
     8041 cgcaggtttg gctaacggtg aaggtaagtg actgctcaca ttcttcaggt cgccgttcag
     8101 ctctacgtta taggtcgcgc cagcatgata aggcagatca atgcccactt taccatccca
     8161 cgccacgctg ccacttaatg cttcgttcac cgcttcaggc agaacgccgg ttttcgctgg
     8221 ttgccagtta ccgttgagat tcaccgcgac ctggtaggct tttgcccctt ctttggtgga
     8281 aaaatccacg ttcaacggct gattaaacca gcttgctgtc agtggttcac tttgcagatc
     8341 gccattgata aagctgaatt taccgctcaa atttttcagg gtgctgtcga gtggtttgat
     8401 aaacagactg ttattacgca gcgtcacatc acctttcgcg gttaccagtt cgccgttcag
     8461 cgggatatca agatgtaagc gagcattcac atcgccgtcg agctggagtt cttgcagggt
     8521 cgcacccaga gaatctttca gcggtgtctc atcaaagtaa gggccaacgg ctttacccgg
     8581 acctttaatg tcagcgtcaa tcagcagttt ttcttttgag tagtcaggga tcactgcggt
     8641 gagattactt gcgcgcacgc cgcccagatt aacgccatcg gttttcatcc ataaaccgtc
     8701 gttaataaag tccagttcaa tatcaaggtt agttaatgca ggccagtccg gctggaaggc
     8761 aaacttcgcg ttgcgcagcg gtaccagcac ttcaaactga ccttcgttgt gtttataggg
     8821 gaagagttgc ggattgccac cataaaccag cgtcgcgtta tccgcttcac cgccctgaat
     8881 cgcgccactt aagtaatcaa ccaggtcttt acccatcaag ttttccggga aatagcgcca
     8941 ggcttgtgaa ccatcatcgg tactgatgcc agccagaata cccagccagg gttcatcgtt
     9001 agctggttgc aggtaacgaa aaccgccgcg cgcatggacg gctttggctt taacgtcaat
     9061 attacgccca tccagctgga aacctttgtc attgttcagc cagcttatcg ttgcctggcc
     9121 gtcggcgatt tccagtggcg cacggaatac cgtttcgtaa ggcatctttg cctgcttcat
     9181 cgacgcagta agcaaaccat tttctacgct gccggaaagc gtcccggaga agtgttccgc
     9241 acccggtaat aatttccatt gcttccaggc cagatcgctc cacgatgcct gaaaacgggt
     9301 cttgtctgcc gcctgaagcg ggatatccag cgccagcgtg ttaatcttgc cgctcggttg
     9361 tgtggagcgc caaacatcac ccagtgcagg tgaaagtttc gcggccagcg ggcgtatgcc
     9421 ctccaggcct gccagctcca gattactggc gcgaatccgg agttcgtcac tgcgtttatt
     9481 gtctttgccg ccaacgtcct gttccggtat ccaggccagc gtcaatgctc cgctcggcca
     9541 gggtttgccg tccatcgtga tccgtgtatc gggaatagag aactgccagc ctggattttc
     9601 acgcgtaata tgcgcggtca gattatccac cgacagcgta tgcgtttgct tctcgcccaa
     9661 ccagctggca ccgccctgtt tcagccagac gtcaccgccg gttacatcgc ctttgtcgat
     9721 cgtcatccag ccttcaaggg agaactgtgc cgtttccagc gcaatattgt cctgcatcca
     9781 tttaccgagc cacggcttca ggtcgatgtc atccgcctgg agccagacgc gaccattgct
     9841 taacaacccc tcatcatcgc gcaaatccat gcgcacctgc atcacgccgt gctgtccggt
     9901 aaggctggag aggcttacct ggccttctgc acggtgtcga cgtggatcgt tcagccaggt
     9961 gagctgtggg atcgccagct cggcgcgctg accggatggc gtcaggaaac tgacttcact
    10021 gtcgcggaga tcgaaatggt cgaactgacg aagaaacaga tcgctgatgt gactggcttc
    10081 cagactatca ttaccaccgc cgctggtgat aggagtgttg gtgcgaaagc gcagctgcca
    10141 gaaagtgagg tcgcgaaact gccagcgcat atgtaacagg ctctgccaga catccagcgc
    10201 cagagtaacg cgtttaaccg aaaattcgcc gccatctttt agttctgcac ggatgtcgtg
    10261 cgcttcaagc gtcggaccaa aattctgcca gctggctgag agctgactgg cttctaccgg
    10321 catgccagtc gcggattcta ttttgttgag gatttccgga cgccaggcgt caagatgcgg
    10381 taaagcaata cgcaggccgc taaccagcag ggcagcgatc acaacgagcg cggctccagt
    10441 aagcagtaaa atccccggca atcgcctcac ccgtcactcc ttgtctgcta aaaatgtgac
    10501 tcaaaaaccc tttgccggat ggcggcccag catctgttta catcattacg acgtcaaact
    10561 gctcctggtt atagagcggt tcaatttgta ctttaacctg tttaccaacg aaaatttcca
    10621 cttccgccag cgagtgtgac tcttcgcctt tcaaggcttc agctactgcc ggagaagcat
    10681 agaccaggaa acggtcggaa tcataagcat ggtgtacacg aacaatctcg cgcatgattt
    10741 catagcatac cgtttccacg gttttcaccg ttccgcgacc gtggcaggtt gggcattcgt
    10801 tacatagtac gtgctcaatg ctttcgcggg tgcgtttacg cgtcatctcc accagcccca
    10861 gcgccgaaaa accattaacg ctggttttca cccggtcttt gctcaacgcc tgctccagcg
    10921 agtgcagcac gcggcggcgg tgatcttcat tattcatatc gatgaaatca ataatgataa
    10981 tcccgcccag attacgcaac cgtaactggc gagcgatagc ctgcgtcgct tcaatattgg
    11041 tattgaaaat ggtgtcgtcc agattgcgat gaccgacaaa cgccccggta ttgatgtcta
    11101 cggtggtcat cgcttcggtc tggtcgataa tcagatagcc accggatttc agttctactt
    11161 tgcgttccag cgctcgctga atttcgtttt cgacatcaaa gagatcgaaa atcggctggc
    11221 gtccggtgta atgctccagc ttgctggtca tctcgggaat gtactccgag gtaaactcca
    11281 gcaatgcttc gtaagtcagg cgtgagtcaa cgcgaatgcg gtcgagttcg gcgtcggcga
    11341 aatcacgcag aacacgctgc gccagcgcca gttcgccgta cagctgataa cgggtctgtg
    11401 ggcgcttttt acgctccata actttggtcc agacgcgttt cagataagcg gcatcggagg
    11461 ccagttcagc ctcgccaacc ccttcagctg cggtacggat gataaacccg ccctgctcgt
    11521 cgcaatactc tgcgaccact tttttcaggc gttcacgttc tgattcgctt tcaattcgtt
    11581 gggaaacccc aacgtgagaa gcccctggca taaataccag atagcgagaa ggcagcgtga
    11641 tatcggtggt caggcgcgca cccttagtgc caagtggatc tttcaccacc tgcaccatca
    11701 gatcttgccc ctgacgaacc agttccgaga tgtcgcgcac cgtgaattgc ttttgttctt
    11761 cacccgccac acattcggtg tgcggcatga tgtcagatgc atgaagaaac gcggctttat
    11821 ccagcccaat atctacaaaa gccgcctgca tacccggaag tacacgactt acacgaccct
    11881 tgtagatatt gcctactatt ccgcgtcgcg cctcacgttc aatatgaatt tcctgcagaa
    11941 taccgccatc aatatacgcc actcgcgttt ccgaaggcgt tacgtttact aacaattcag
    12001 ccgtcatgtt tatccctttt ctcacgcagt gcgttaaaat tacttaataa ttcatacgtt
    12061 tcaaccagcg gtaagccgac tacggcgtga tagctgccat ttatcttcct gacaaaacag
    12121 ccacccagcc cctgaatacc gtatgcacct gctttatcta acggttcacc gctggcgaca
    12181 tagcccgcga tgtcttcgtc tgttaacgtt ctgaaagtca catcggtgac caccaggcaa
    12241 tcgagaatgt gctggctgtc ggccaacgcc actgccgtca ttacctgatg ggtctgaccc
    12301 gataatttgc gcaacatctg cgccgcatgc tctgcgtcgc gcggtttctc cagtacttct
    12361 ccgttcagga taacgatagt atccgcaccc agcaccggga gatcctgcgc cgtttgcgcg
    12421 acacccgcct gtgctttctc gcgcgccaga cgcacaacat actgctgcgc gctctcctgc
    12481 ggctgacgct gctcctcaat gcccgtaaca atacgttcaa aggttacgcc aagttgcgca
    12541 agtaactcct gacgacgcgg agaaccggaa gctaaataca gagaagtcat agaaaccttt
    12601 attgcactgc aaactgctga cggactttac gcatcagcaa gaaaatccac ggccagagca
    12661 ccccattgac tacgctactc cagaacactt ccggtctgaa agagacgtta atcactaaaa
    12721 actctgccca gaaaacaata atatccacca ccagcgaaag caacatgacg accagcgcct
    12781 gctgccataa tgcgaggttg cggaaaagct ggtatttcag cgccaccagg taagcaatga
    12841 tgctcatcgc caatacgcgt acgccaagcg tcgagccgct gatcagatcc agtatggcac
    12901 ccatcacaaa acctgtgccc acatttacgc gatgaggcaa ggccaggatc caatacaaca
    12961 agatgagtaa cacccagttt ggccggaaaa caatcaggtt atccggccag ggcatgattt
    13021 gcagcaacag cgcaatgagg aaagagagcc agattaccca gcgtccctgg ctacgatagc
    13081 tcgccactat tgccctcccg gcgcacgcgc aggcggttga gcaccacttt gcggcggcgt
    13141 agccctttgt ggagagcgat tagcagcggg ctgtgtgggc gcagcaggcg cagtaactgc
    13201 atttcctgtc gccggttgct gcggagtcgg ctgagtgatc cccgttgccg gttcaggtaa
    13261 ctttggcccc atcgcgtctg gcgaaggcaa tacctgcggc atcatctgca tcaaacgttc
    13321 attagcaaca cgatgcacct cttccggcgt catcgggtta gcgccgttac gatccgcgcc
    13381 ccacagcagc agcagataac gcaaacgttg cagtcctgca gtcggacgcg cctgaatcac
    13441 agtataagcg cgctgggtat cgagttttac ggaagagaca accgcgaccg gatagccttc
    13501 cgggaaacga ccgcccagac cggaagtcac cagcacatca ccaacacgaa tgtccgtatt
    13561 cgctggcaga tgctcaagct gcaaatcatc cgtacaaccg ttaccggctg caattacgcg
    13621 gatatcgttg cgcagcacct ggattggcag cgcgtgggtc gcatcacaaa tcagcagcac
    13681 gcggctggtc agtttagcga cggccaccac ctgaccaaca acacctttgt cgctgataac
    13741 cggctggcct tcataaacgc cattaacgct acctttatcg ataacaactt gatcgctata
    13801 aggatcgtta accgtggaga taacctgagt caccattttc tgctcatcct gacgcagcgg
    13861 ggaacccagt agctcgcgca gacgcgcgtt ctcctgtttg tattgtccaa gcatcagcag
    13921 ttcactgttt ttcagcaaca gttcctgacg taacgcccgg ttttcaagtt ctagttggtc
    13981 acgcgaggcc agcgtctgcg atacgccatc cagcaattca cgaggagcat tggaaacaaa
    14041 gtagaaagga ctgacggcgg tatccatata agtacggatt tgactgaacg tccccaggcg
    14101 gctgtcggca ataataatgc cgagcgccac cagcaccgcc agaataaggc gaatctgtag
    14161 cgacgggcca cggctaaaaa ttggcttcat aagttatgcg tattctcgta tcagaccagg
    14221 cagggtaaac agacacttcc cctgcctgca tccgattact cttcgctgaa caggtcgccg
    14281 ccgtgcatgt cgatcatttc cagcgctttg ccgccaccgc gcgccacaca ggtcagcggg
    14341 tcttcagcaa caacgactgg aatgccggtt tcttccatta acaaacggtc aaggttacgc
    14401 agcagtgcgc caccaccggt aagcaccatg ccacgctcgg agatgtcgga agccagttcc
    14461 ggcgggcact gttccagtgc aaccattacc gcgctcacaa taccggtcag cggttcctgc
    14521 agtgcttcga ggatttcgtt ggagttcagg gtaaagccgc gtggaacacc ttctgcaagg
    14581 ttacggccac gaacttcgat ttcacgtact tcatcgcccg ggtaagccga accgatttcg
    14641 tgcttgatac gttctgcggt ggcttcaccg atcagagaac cgtaattacg acgtacatag
    14701 ttgatgatag cttcgtcgaa acggtcacca ccaatgcgca cggaagagga gtaaaccacg
    14761 ccgttcaagg agataacagc aacttcagta gtaccaccac cgatatcaac caccatagaa
    14821 ccggtcgctt cagaaaccgg caggccagca ccaattgcgg cagccatcgg ttcttcaatc
    14881 aggaagactt cacgggcacc agcgccctgc gcggattcac gaattgcgcg gcgttcaacc
    14941 tgggtcgcgc caaccggcac acaaaccaga acgcgcgggc ttggacgcat aaagctgttg
    15001 ctgtgcactt gtttgatgaa gtgctggagc attttttcag tcacgaagaa gtcggcgata
    15061 acgccgtctt tcattgggcg aatagcagca atattgcccg gcgtacggcc cagcatctgc
    15121 ttcgcgtcat gacctactgc agctacgctt ttcggtgaac cggcacgatc ctgacgaatg
    15181 gccaccacgg aaggctcatt caatacgatg ccttgtcctt ttacataaat gagggtattc
    15241 gcagtaccca ggtcaatgga caagtcattg gaaaacatgc cacgaaattt tttcaacata
    15301 ctaagggata atcctgaaag ctggggcgga aaagaaaatc cgcttacttt accaaccaca
    15361 cgcagcagcg acaaggcgca aaaatcatct gctacggtga aaattagtgc agttcgtttc
    15421 ctttgttaca aatctctgcc tgagtccaga aaggcttaat gcatcagcag cattcctcgc
    15481 ctgtttgcaa ccgcgtaagg tcattcatct gcatatgtgc tgcaacaatc tggcgagcag
    15541 acaagcacac tcccatgaga cgcagcgcgc gttattctac gtgaaaacgg attaaacggc
    15601 aggttaaacc gagtatcttt gtgaatattt tttcacgtta gtatcaagtg gctgtgagga
    15661 cgcgaaaaaa tccccttgcc cacctgtaac accgcgctga atcagggtct gccactcgct
    15721 tcgcgaacgc acgccggtgg cgtaaacctg ggtgctggtc ccggagcagg cttccaccag
    15781 gctttgaacc agcagctgat tctccgttcg cttctcaatg tttctgacca gccccggatg
    15841 gagcttgagt aactcaacat taagttcttt gatccaactg gtgcttacca gcgtcaaacc
    15901 agcctggttg acggctaccc gtacccctaa agcattcact aaacgaataa caggttgtaa
    15961 gcgactgata tgttgaccaa catcggcctc tgcaagttca ataattatgc gtcttcgttg
    16021 cgatttttca cattgcatta acgtatcgcg cagccaacgc tgaaaacgcg ggcgaatcag
    16081 cgactcaacg gtaacctgaa tcgccagatt ttcctctggc cagtaacgca atagtggaat
    16141 aagacggctg attagcaggc ggtcatactc ttccgataaa ccaaactgca agaccatcgg
    16201 catatactct gccgagctaa cctcttcatt accatcgaag atgcggcaca tgagttcacg
    16261 atgatgaacc cggccttcgc gagtaaccgc cggtttttga taaaggcgcg ggccgccgcg
    16321 actgagcatt tgctcgataa gcgtacgcca gcgaacatta ccgcgtcctt tttcaggcaa
    16381 cgagtcatcg taaatagccc agctattgcc gccctgcaat cccgcattac gcgtggcaga
    16441 ctctgcatgt tccattacct gctcggtatc ctgaccacta cgccaggcac agataccaat
    16501 gtggatcata tcgtcgcgat cgagcatttt attgttcggc aaggtatcga cggctttgat
    16561 taactgactg gcgatgctct ctgcctcttt taacgtccgg tgcggtaaca gcgcagcaaa
    16621 atcactgcgg tggtaacgcg ccagcagtgc gccagggtag cgcatcataa atgtcgatag
    16681 cagattcgtc agagagaaga actgttcttc aacctggctg tgcccccagg tatcactcaa
    16741 catattgaaa tccggcagac gaatcatcat cacgatcccg tgggtaccta ctttctcttg
    16801 atcttccagt aacgttgcta actgattatc gaaaaagagt cggttattga ggccggtttt
    16861 catgtcctgg gcggcataag agcggatcag cgtatcaaga cggctgtgtt gttcgcgtgc
    16921 gttctgaatt tcacaaagca gcgtatccag cgcactgctg gttctgggcg gccattcata
    16981 gatggttccc aacacattag agccacgctc accgtttaag atacgagtag cccgggtttc
    17041 cagcaattct tgcccggcaa gttgccgttg taaccagcgt accgccagga agagcataag
    17101 aatgataaag ccaatcgccc ccgtgagcgg cgcggtagtc attaaagaat ggaaatagtt
    17161 gcccatcgga tcctgataaa ccagacgcag cgacatcccc ggatgcttta tcaacggaac
    17221 gctcagttca cgaaacagat cgttggtgcc aaccggacga taactaccat ttctggccag
    17281 ggtataaacc tgcttatcac catggagcag gtctacacga acgatatctg ccgacatcat
    17341 taattcggta atttgtggcc ttaatgtgct gaagtcattc gacactaggt gggtatcaat
    17401 cgccgtcgcc accgcctgaa cacgatgact aaacttatac tgaatggcgt tgtagaaact
    17461 tagcgaacag cccagcaaag tcacaaaaat tgttaacccg gtgagcagcg tgacaaaggc
    17521 cgaaaatttc gtcgttaatc tcatccttgt gttaactccg atagtgagga agcgggcata
    17581 ctagcaaatc agatttatct cgcaatttat tgcgcttcat cggctttgct tttccattag
    17641 cgagtatagt cttcagaaat tattttccaa tccatcatgc acatgaggac cacttatgca
    17701 ggcgttactt ttagaacagc aggacggcaa aactctcgca tcagtacaga ctctggacga
    17761 aagtcgcctg ccggaaggcg atgtcacggt tgatgttcac tggtcgagcc tgaactacaa
    17821 agatgcgctg gcgattaccg gtaagggaaa aatcatccga aattttccga tgattcctgg
    17881 gattgatttt gccggaactg tacgcaccag cgaagatccg cgttttcatg ccggtcagga
    17941 ggtgttactc actggctggg gcgttggtga aaaccactgg ggtgggctgg cggaacaggc
    18001 gcgagtgaaa ggtgactggc tggttgccat gccgcaaggg ctggacgcgc gtaaagcaat
    18061 gattatcggt actgccggtt ttaccgccat gctgtgtgtg atggcgctgg aagatgccgg
    18121 tgttcgcccg caggatgggg aggttgtcgt gacgggtgcc agtggtggcg tcggcagtac
    18181 cgccgtcacg ctgctgcata agttgggtta tcaggtcgtt gccgtttccg gtcgcgaaag
    18241 tacccatgaa tatctgaaaa gtttaggtgc cagccgtatt cttcctcgcg atgagtttgc
    18301 tgaatcccgt cctctggaaa aacaagtctg ggctggggca attgacaccg ttggcgacaa
    18361 agtgctggca aaagtgctgg cgcaaatgaa ttacggcggc tgcgtggcgg cctgtggtct
    18421 ggcgggtggt tttactctgc caaccacggt catgccattt attctgcgta atgtccgttt
    18481 gcaaggggtg gattcagtaa tgacgccacc agcacgccgc gcacaagcct ggcagcgact
    18541 ggtcgccgat ttaccggaat cattctatac ccaagcggca aaagagatat ctctggcaga
    18601 ggcaccgaag tttgccgagg ccatcattaa taaccagatc cagggtcgca cgctggtgaa
    18661 ggttaactaa ccatttagca gggaataata agagagggaa ctcattttga aatcattatt
    18721 caatcggtta acgggaaaag cggttagccg gacagctttc gtcgaacatc ttggtcatga
    18781 agttgtacaa catcatccaa actggaaagt catgatttcg actgaccata aattgatgcg
    18841 cattgatgct ccaccaaaca gccattattg atacgcctcc gtcgcctgtt aggtttatgg
    18901 tgttttgcct tggcgacgct acgcttaatc tgttactgat ttctggtacc atggggggaa
    18961 taatctgatt ttatttgatt gcaaattaac cacttaaacc tattgaatgc ggaacattgt
    19021 cagtcggtta attttgcgtg ctttagcatt cacatctttc cggacgatgc agtgaaaatt
    19081 gcgtaatcct cagcaaccgc tgcgtaatgt cgtctatctt gtcgcgatcc tggcatccct
    19141 acattatttg tggtctgtga agattatctc attgcagccc ctcatcttcg cagggctggc
    19201 tttacagctt ttcaccctac gttataagaa gttccgtcga tgatggcgct aatttcgtga
    19261 attgtgcggc ttgttgcaaa ttacacggtg ttgaaggtta tttacatgtt agctgttgat
    19321 tatcttccct gataagacca gtatttagct gccaattgct acgaaatcgt tataatgtgc
    19381 gacctcgtcc tccctgacgc agtttttgcg ctgcggaaaa ggtgacattg gcgcaacgaa
    19441 ggtatatttt gttttttgcc ggaggatagc agcagatcgc tgcacaatgt ccgtcaagtc
    19501 taacattgac actctggggc aaaatagacc ggcgtcccgg cctgctggaa tttatcgcta
    19561 tgcatacagc tgtcggggca tacgctttac agacggcggt gaaacgcctg tcacaatcac
    19621 actaaacaaa gagtacggaa cccactcatg gatattcgta agattaaaaa actgatcgag
    19681 ctggttgaag aatcaggcat ctccgaactg gaaatttctg aaggcgaaga gtcagtacgc
    19741 attagccgtg cagctcctgc cgcaagtttc cctgtgatgc aacaagctta cgctgcacca
    19801 atgatgcagc agccagctca atctaacgca gccgctccgg cgaccgttcc ttccatggaa
    19861 gcgccagcag cagcggaaat cagtggtcac atcgtacgtt ccccgatggt tggtactttc
    19921 taccgcaccc caagcccgga cgcaaaagcg ttcatcgaag tgggtcagaa agtcaacgtg
    19981 ggcgataccc tgtgcatcgt tgaagccatg aaaatgatga accagatcga agcggacaaa
    20041 tccggtaccg tgaaagcaat tctggtcgaa agtggacaac cggtagaatt tgacgagccg
    20101 ctggtcgtca tcgagtaacg aggcgaacat gctggataaa attgttattg ccaaccgcgg
    20161 cgagattgca ttgcgtattc ttcgtgcctg taaagaactg ggcatcaaga ctgtcgctgt
    20221 gcactccagc gcggatcgcg atctaaaaca cgtattactg gcagatgaaa cggtctgtat
    20281 cggccctgct ccgtcagtaa aaagttatct gaacatcccg gcaatcatca gcgccgctga
    20341 aatcaccggc gcagtagcaa tccatccggg ttacggcttc ctctccgaga acgccaactt
    20401 tgccgagcag gttgaacgct ccggctttat cttcatcggc ccgaaagcag aaaccattcg
    20461 cctgatgggc gacaaagtat ccgcaatcgc ggcgatgaaa aaagcgggcg ttccttgcgt
    20521 accgggttct gacggcccgc tgggcgacga tatggataaa aaccgcgcca ttgctaaacg
    20581 cattggttat ccggtaatta tcaaagcctc cggcggcggc ggcggtcgcg gtatgcgcgt
    20641 agtgcgcggc gacgctgaac tggcacaatc catctccatg acccgtgcgg aagcgaaagc
    20701 tgctttcagc aacgatatgg tttacatgga gaaatacctg gaaaatcctc gccacgtcga
    20761 gattcaggta ctggctgacg gtcagggcaa cgctatctat ctggcggaac gtgactgctc
    20821 catgcagcgc cgccaccaga aagtggtcga agaagcacca gcaccgggca ttaccccgga
    20881 actgcgtcgc tacatcggcg aacgttgcgc taaagcatgt gttgatatcg gctatcgcgg
    20941 tgcgggtact ttcgagttcc tgttcgaaaa cggcgagttc tatttcatcg aaatgaacac
    21001 ccgtattcag gtagaacacc cggttactga aatgatcacc ggcgttgacc tgatcaaaga
    21061 acagctgcgt atcgctgccg gtcaaccgct gtcgatcaag caagaagaag ttcacgttcg
    21121 cggccatgcg gtggaatgcc gtatcaacgc cgaagatccg aacaccttcc tgccaagccc
    21181 gggcaaaatc acccgtttcc acgcaccagg cggttttggc gtgcgttggg agtctcatat
    21241 ctacgcgggc tacaccgtac cgccgtacta tgactcaatg atcggtaagc tgatttgcta
    21301 cggtgaaaac cgtgacgtgg cgattgcccg catgaagaat gcgctgcagg agctgatcat
    21361 cgacggtatc aaaaccaacg ttgatctgca gatccgcatc atgaatgacg agaacttcca
    21421 gcatggtggc actaacatcc actatctgga gaaaaaactc ggtcttcagg aaaaataaga
    21481 ctgcaaaagc gtcaaaaggc cggattttcc ggcctttttt attactgggg atcgacaacc
    21541 cccataaggt acaatccccg ctttcttcac ccatcaggga caaaaaatgg acactcgttt
    21601 tgttcaggcc cataaagagg cgcgctgggc gctggggctg acccttttgt atctggcggt
    21661 atggttagta gccgcttact tacctggcgt tgcccccggt tttaccggtt ttccgcgctg
    21721 gtttgagatg gcctgcatcc tgacgccgct gctgtttatt ggactgtgct gggcgatggt
    21781 gaaatttatc tatcgcgata tcccactgga ggatgacgat gcagcttgaa gtaattctac
    21841 cgctggtcgc ctatctggtg gtggtgttcg gtatctctgt ttatgcgatg cgtaaacgga
    21901 gcactggcac cttccttaat gagtatttcc tcggcagccg ctctatgggc ggtattgtgc
    21961 tggcgatgac gctcaccgcg acctatatca gtgccagttc gtttatcggc gggcctggag
    22021 ctgcttataa atacggcctg ggctgggtat tgctggcgat gattcagctt cctgcagtct
    22081 ggctttcgct cggtattctc ggcaagaagt tcgcaattct tgcgcgccgc tacaatgcgg
    22141 tgacactgaa cgacatgctg tttgcccgct accagagtcg tcttttggtg tggctggcga
    22201 gtttgagttt gctggtcgcg tttattggcg cgatgaccgt gcagtttatc ggcggcgcgc
    22261 gcctgctgga aaccgcggcg ggtattcctt atgaaaccgg gctgctgatt ttcggtatca
    22321 gcattgcgtt gtataccgcc tttggtggct ttcgcgccag cgtgctgaac gacactatgc
    22381 aagggctggt gatgctgatt ggcaccattg tgctgcttat tggcgtggta catgccgcag
    22441 gtggcttaag caacgcagta gagacgttgc aaaccatcga tccgcaactg gttacgccac
    22501 aaggcgctga cgatattctg tcgcctgcct ttatgacgtc gttctgggta ctggtgtgct
    22561 ttggcgtgat tggcctgccg catactgcgg tgcgctgtat ctcttataaa gacagcaaag
    22621 ccgtacatcg ggggatcatc atcggtacga ttgtggtcgc aatcctgatg ttcggtatgc
    22681 acctggccgg agcgttaggt cggacggtga tccccgatct caccgtaccg gacctggtga
    22741 tcccgacgtt aatggtaaaa gtgttgccac cgtttgctgc cgggatcttc ctggctgcac
    22801 ctatggctgc gatcatgtcg acaattaacg cccaactgct gcaaagttcc gctacgatca
    22861 ttaaagatct ctatctgaat atccgtccgg atcaaatgca aaacgagacg cgtctgaagc
    22921 ggatgtcggc ggtaattacg ttagttctcg gtgcgttgct gctgcttgcc gcctggaagc
    22981 cgccagaaat gatcatctgg ctgaatttgc tggccttcgg tgggctggaa gccgttttcc
    23041 tgtggccgct ggtactgggt ctttactggg aacgcgccaa cgccaaaggc gcgctaagtg
    23101 cgatgattgt tggcggcgta ctgtatgccg tactcgcgac gctgaatatt cagtacctgg
    23161 gcttccaccc tatcgtgccc tcgttactac taagtttgct ggctttcctg gtcggaaacc
    23221 gtttcggtac atccgtcccg caagctaccg ttttgactac tgataaataa agagttttgc
    23281 catgccttgg atccaactga aactgaacac caccggcgcg aacgcggaag atcttagcga
    23341 tgcgctgatg gaagcgggtg ccgtttctat cacttttcag gatacccacg atacgcctgt
    23401 gtttgagccg ctgccgggcg aaacgcgcct gtggggcgac accgatgtga ttggtctgtt
    23461 cgacgctgaa accgatatga acgacgtggt ggcgattctg gaaaaccatc cgctgctcgg
    23521 cgcgggcttc gcgcataaaa tcgaacaact ggaagataaa gactgggagc gcgaatggat
    23581 ggataatttc cacccgatgc gctttggtga acggctgtgg atctgcccaa gctggcgtga
    23641 tgtgccggac gaaaacgccg tcaacgtgat gttagatcca gggctggcgt ttggtacggg
    23701 tacccatcca actacctctc tgtgcctgca gtggctcgac agcctcgatt taaccggtaa
    23761 aacagtcatc gactttggct gcggttccgg cattctggct atcgcggcgc tgaaactggg
    23821 tgcagcaaaa gccattggta ttgatattga tccgcaggcg attcaggcca gccgcgacaa
    23881 cgcggaacgt aatggcgtct ctgaccgtct ggaactctac ttaccgaaag atcagccaga
    23941 agaaatgaaa gccgacgtgg tggtcgctaa catccttgca ggcccattac gtgaactggc
    24001 accgttaatc agcgtcctgc cagtttcagg cggtttgctg ggcctttccg gtattctggc
    24061 aagccaggca gagagcgttt gcgaagctta tgccgacagc ttcgctctgg acccggtcgt
    24121 ggaaaaagaa gagtggtgcc gcattaccgg tcgtaagaat taaccttcgc atcgccgtag
    24181 ggtgacgcag agcgaagtgc gagcgatgtc acaaaaggca cgttaatttg ccgattattt
    24241 acgcaaattt gcgtgccaaa attttcattc ataaagaaaa attgagaact tactcaaatt
    24301 tcttggagtg taaattttag tcactatttt ctaatatgat gatttttatg agtaattatc
    24361 gcgccacgct cattttaaat gcaattcttt gatccatctc agaggattgg tcaaagtttg
    24421 gcctttcatc tcgtgcaaaa aatgcgtaat atacgccgcc ttgcagtcac agtatggtca
    24481 tttcttaact catgcgcatc ggacaatatc agctcagaaa tcgcctgatc gcagcgccca
    24541 tggctggcat tacagacaga ccttttcgga cgttgtgcta cgagatggga gccggattga
    24601 cagtatccga gatgatgtct tctaaccctc aggtttggga aagcgacaaa tctcgtttac
    24661 ggatggtgca cattgatgaa cccggtattc gcaccgtgca aattgctggt agcgatccga
    24721 aagaaatggc agatgcagca cgtattaacg tggaaagcgg tgcccagatt attgatatca
    24781 atatgggttg cccggctaaa aaagtgaatc gcaagctcgc aggttcagcc ctcttgcagt
    24841 acccggatgt cgttaaatcg atccttaccg aggtcgtcaa tgcagtggac gttcctgtta
    24901 ccctgaaaat tcgcactggc tgggcaccgg aacaccgtaa ctgcgaagag attgcccaac
    24961 tggctgaaga ctgtggcatt caggctctga ccattcatgg ccgtacacgc gcctgtttgt
    25021 tcaatggaga agctgagtac gacagtattc gggcagttaa gcagaaagtt tccattccgg
    25081 ttatcgcgaa tggcgacatt actgacccgc ttaaagccag agctgtgctc gactatacag
    25141 gggcggatgc cctgatgata ggccgcgcag ctcagggaag accctggatc tttcgggaaa
    25201 tccagcatta tctggacact ggggagttgc tgcccccgct gcctttggca gaggttaagc
    25261 gcttgctttg cgcgcacgtt cgggaactgc atgactttta tggtccggca aaagggtacc
    25321 gaattgcacg taaacacgtt tcctggtatc tccaggaaca cgctccaaat gaccagtttc
    25381 ggcgcacatt caacgctatt gaggatgcca gcgaacagct ggaggcgttg gaggcatact
    25441 tcgaaaattt tgcgtaaaca gaaataaaga gctgacagaa ctatgttcga acaacgcgta
    25501 aattctgacg tactgaccgt ttctaccgtt aactctcagg atcaggtaac ccaaaaaccc
    25561 ctgcgtgact cggttaaaca ggcactgaag aactattttg ctcaactgaa tggtcaggat
    25621 gtgaatgacc tctatgagct ggtactggct gaagtagaac agcccctgtt ggacatggtg
    25681 atgcaataca cccgtggtaa ccagacccgt gctgcgctga tgatgggcat caaccgtggt
    25741 acgctgcgta aaaaattgaa aaaatacggc atgaactaat tcaggttagc taaatgcttg
    25801 attaaaaagg cgctactcgg catggggaag cgcctttttt ataggtgtca caaagggagt
    25861 gaccatgaga acaggatgtg aaccgacccg atttggtaat gaagcaaaga ccatcatcca
    25921 gggtgatgca ttaaccgaac ttaaaaagct ccccgccgaa agcgtcgatc tgatctttgc
    25981 cgacccaccg tataacatcg gtaaaaattt tgatggtctg atcgaagcct ggaaagaaga
    26041 tctgtttatc gactggctgt ttgaagtgat tgcagagtgc caccgcgttc tgaaaaagca
    26101 gggcagcatg tacatcatga acagcacgga aaacatgccc tttatcgatc tccagtgccg
    26161 caagcttttt accatcaaaa gtcgcatcgt ctggtcatat gacagttcag gagtgcaggc
    26221 gaaaaaacac tacggctcca tgtacgaacc catcctgatg atggtgaaag acgcaaagaa
    26281 ctacacattc aacggtgatg ctgttctggt agaagccaaa accggatcgc agcgcgcgtt
    26341 gatcgattat cgcaaaaatc ctccacagcc ttacaatcat caaaaagtac cgggtaacgt
    26401 ctgggatttt cctcgcgtgc gttatttgat ggatgaatat gaaaaccacc cgacgcaaaa
    26461 accggaagcc ttactgaaac gcattattct cgcctcttcc aacccaggcg atatcgttct
    26521 cgacccgttt gccggtagct ttactaccgg tgccgtagcc gtcgccaacg ggcgaaaatt
    26581 cattggtatt gagatcaaca gcgagtacat caaaatgggg cttcgacggc tggatgtcgc
    26641 gtcgcattac tctgcggaag aactggcgaa agtgaaaaaa agaaagaccg gcaacctgtc
    26701 aaaacgaagc cggttaagcg aagttgaccc cgatctcatt gcaaagtaaa ggaagtgtaa
    26761 gcctggtttt cagattattc atttcgtgta tatttcctgc cagacttggt taaacatgca
    26821 caggctcagg taatgattcg caagtattgg tggctcgtcg ttttcgctgt cttcgttttt
    26881 ctgtttgata ctttactgat gcagtggatt gaactgctgg caacagaaac agacaaatgc
    26941 cgcaatatga actcagttaa tccactaaaa ctggttaact gtgacgaact gaattttcag
    27001 gacagaatgt gaatttatat gacacttaat tcattcgttt tatgaattaa tttcgttatg
    27061 ttttcatctg gcatgaacat tcttaatacg ttatcgacca gagcgggggc ttgtttataa
    27121 agatcataac ccgccatatt cattaaccag ttttgaacaa ttccgctgaa ggcaccatca
    27181 ataataatca tcacaacatc taaatcgaga ttatttgcta cacaaccttg ttgctgacac
    27241 gcctgcaata cttcgcggag agtctgcgga ttaaagccca tcttttcgcg tatcactccc
    27301 tcggccagca tctcatcatt aaattcacat ttgtgatata agattttcag caacgcctgc
    27361 tggcggggaa ttttggcaat atattgcaaa ccgacaatca atttttcacg caattgctga
    27421 aacgggtcat gctctaaccc agcggtcaag tgatcctgga ttaactcccg caatgaaggc
    27481 tgttgcaacc acatctcatt aaacagttgg gtcttgttct cgaagtgcca gtagatagcg
    27541 ccacgcgtaa cgttagcagc gtcggcaatg tcgttgagcg tcgtcttgct tacgccatgt
    27601 tgcgcaaact gggcgatggc agtttcaatc agttcttgcc gggtcttcag agcttcggct
    27661 ttggttcttt ttgccatgat taattattca ggaaataaat atattcgaca cagagtgaga
    27721 aaatagcgaa ggttaatcta tcacctaatg tgtatttata cgagaggcta atattgagtt
    27781 gctat
//
LOCUS       DAZGAP010000052        27686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGAP010000052 DAZGAP010000000
VERSION     DAZGAP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.052"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1857)
                     /gene="traD"
                     /locus_tag="ACHLAM_001851"
     CDS             complement(<1..1857)
                     /gene="traD"
                     /locus_tag="ACHLAM_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO7853384.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHSGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQA"
     gene            complement(1914..2645)
                     /locus_tag="ACHLAM_001852"
     CDS             complement(1914..2645)
                     /locus_tag="ACHLAM_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853385.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYSPVIQDNTATTKDYLYSNNI
                     DADLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAQEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDRINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            complement(2813..3073)
                     /locus_tag="ACHLAM_001853"
     CDS             complement(2813..3073)
                     /locus_tag="ACHLAM_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853386.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            complement(3133..3864)
                     /gene="traT"
                     /locus_tag="ACHLAM_001854"
     CDS             complement(3133..3864)
                     /gene="traT"
                     /locus_tag="ACHLAM_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO7853387.1"
                     /translation="MKMKKLMIVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVSSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSEVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3913..4392)
                     /locus_tag="ACHLAM_001855"
     CDS             complement(3913..4392)
                     /locus_tag="ACHLAM_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO7853388.1"
                     /translation="MKNSAHIALITVIQLIACYLADWGSAETVFILCFIVLWQGLFIW
                     LFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMTFIIGTLYELRRVCGCVSV
                     REWMQSQVNEKTNEDLHLDFDNMDFHRTNPATGLPMLGIGVDIGGNLYGYSSKRYDE"
     gene            complement(4414..7257)
                     /gene="traG"
                     /locus_tag="ACHLAM_001856"
     CDS             complement(4414..7257)
                     /gene="traG"
                     /locus_tag="ACHLAM_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO7853389.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLINVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMVADDPYMVIFSQPSPLRQVPNNNYSFLQSSEPFVSCK
                     DASVGLKDKLNLDTKTGGKTWHYYVQQLFGGRPDPDLLFRELLSDSYSYFYGASQSAS
                     QIMRKNVTINALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSVGHVIMRNLPM
                     VQTILTGITIGIFPLLVLAAAFNKMTLPVLKGYVFALMWLQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSEVSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASS
                     SITSTASAAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRD
                     GSMVMDASGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRST
                     RASAGMYGDAHAEWGIKPKILGVGGGAGVRGGGRAGIDWEDNDAHTASSNTQSSHNAR
                     HDIDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRR
                     AMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQ
                     DSNIRNDVKQQVDNMVTEYRGNIGDTQNSILGEENIVRGQYSELQNHHKTEVLSQDNK
                     YNEEKSAQERMPGADSPQELMKRAKEYQDKHKQ"
     gene            complement(7254..8627)
                     /gene="traH"
                     /locus_tag="ACHLAM_001857"
     CDS             complement(7254..8627)
                     /gene="traH"
                     /locus_tag="ACHLAM_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO7853390.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQESQQKVCQDIAGESNIFADWAASRQGCTVGGQSSAVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDIVRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTISKDRALKSQIAKLLTSIQNKALSDSPLAENEKTF
                     IQSTTIPVFRYLVDPLMLGVSNSVLYQLTDYIGYDIMLQYIQELLQQARAMISTGNYP
                     QAVLDSVLDNLNQAQQQIAIFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGGVQ"
     gene            complement(8614..9006)
                     /gene="trbF"
                     /locus_tag="ACHLAM_001858"
     CDS             complement(8614..9006)
                     /gene="trbF"
                     /locus_tag="ACHLAM_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO7853391.1"
                     /translation="MKENKKNQEFKIRKIKRDIERGCDNAIKHFWLFVVFFVAGLLLW
                     TVMNVIFDACIDSWKADPELNNVRDMWNILMYAIPCTLLALAAGFLVTFFLSPMFELI
                     FGNIMIFLLKRRMRRENKLREGGNNASH"
     gene            complement(8960..9333)
                     /gene="trbJ"
                     /locus_tag="ACHLAM_001859"
                     /pseudo
     CDS             complement(8960..9333)
                     /gene="trbJ"
                     /locus_tag="ACHLAM_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
     gene            complement(9263..9808)
                     /gene="trbB"
                     /locus_tag="ACHLAM_001860"
     CDS             complement(9263..9808)
                     /gene="trbB"
                     /locus_tag="ACHLAM_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO7853392.1"
                     /translation="MFPVKSLSVVLLFLASCTQASTRAEIERLWHPEGMAARTTQPAA
                     DAQARQEKAATRWFRLSNGTQVNLADWKVVLFMQGRCPYCHQFDPVLKQLALQYGFSV
                     FPYTLDGQGDTAFPQALPAPPDVLKTFFPNIPVATPTTFLVNVNTLEALPLLQGASDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(9795..10082)
                     /gene="traQ"
                     /locus_tag="ACHLAM_001861"
     CDS             complement(9795..10082)
                     /gene="traQ"
                     /locus_tag="ACHLAM_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO7853393.1"
                     /translation="MCKYKFSLPRLDITGMWVFSLGVWFHIVARLVNSKPWMAYILAQ
                     VISAVLVLWGAYRVLDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
     gene            complement(10148..10549)
                     /locus_tag="ACHLAM_001862"
     CDS             complement(10148..10549)
                     /locus_tag="ACHLAM_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853394.1"
                     /translation="MDKENCSISERWLIASGVAVILLTLLAFMSFPDPDVSMNRYYSW
                     LMNVLILSVTGVMYVALSVVMVMLYFIYERNSRLLYLSVLALIIAAGIWLLRFLDDFL
                     MTAAFYVALFVLWVFLCFLYIQCENEMSMSE"
     gene            complement(10680..11045)
                     /locus_tag="ACHLAM_001863"
     CDS             complement(10680..11045)
                     /locus_tag="ACHLAM_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853395.1"
                     /translation="MSFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCTLSMM
                     MAEGIVIMKYELWHIPPALLGLAGVLSWLLLLLHTGYFSGISEGEHKRLWLAVAAVFS
                     GAGMIIFYWLLQPSETIMK"
     gene            complement(11059..11802)
                     /gene="traF"
                     /locus_tag="ACHLAM_001864"
     CDS             complement(11059..11802)
                     /gene="traF"
                     /locus_tag="ACHLAM_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO7853396.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     SRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIARLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11795..12052)
                     /locus_tag="ACHLAM_001865"
     CDS             complement(11795..12052)
                     /locus_tag="ACHLAM_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO7853397.1"
                     /translation="MKVIFTSNRLVDFLIRLVITAIVISPVIIWSWDTVKETTAGGVL
                     AAVFVILYSGVLLFILYFCFFALTDLQKADERKSDERNEDE"
     gene            complement(12066..13970)
                     /gene="traN"
                     /locus_tag="ACHLAM_001866"
     CDS             complement(12066..13970)
                     /gene="traN"
                     /locus_tag="ACHLAM_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO7853398.1"
                     /translation="MKEKINTFFRSLNVNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWA
                     TSETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTARIIGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSAHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLT
                     GSGCTANGNCLPHGHGDRMVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     GDGVLKGTECSEPGGTKTGVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGSCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(14064..14444)
                     /locus_tag="ACHLAM_001867"
     CDS             complement(14064..14444)
                     /locus_tag="ACHLAM_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853399.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPP
                     YIAFVCPVKMAALLERRLRQDAESAIPRLAWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(14441..15079)
                     /gene="trbC"
                     /locus_tag="ACHLAM_001868"
     CDS             complement(14441..15079)
                     /gene="trbC"
                     /locus_tag="ACHLAM_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO7853400.1"
                     /translation="MNKKIYILFALTAGLFISAHASENVNTPENRQFLKQQETLSQQL
                     RENPDTQLQAWAEQQVRANPLQHADNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKPQ"
     gene            15272..15511
                     /locus_tag="ACHLAM_001869"
     CDS             15272..15511
                     /locus_tag="ACHLAM_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853401.1"
                     /translation="MNNSTSEDRPDFSFSYMDNQRADEIKALKILLFSIIRNMPHDTA
                     ISIINNIVNTDNNEALNRLCLEITDAMLNTTKIVD"
     gene            complement(15508..15687)
                     /locus_tag="ACHLAM_001870"
     CDS             complement(15508..15687)
                     /locus_tag="ACHLAM_001870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853402.1"
                     /translation="MMEKEQKQCGPQLPLHQDEAPPAFQVTYSIEKKEITAEQYRDWQ
                     LIAASLRKKINGERS"
     gene            complement(15708..16700)
                     /gene="traU"
                     /locus_tag="ACHLAM_001871"
     CDS             complement(15708..16700)
                     /gene="traU"
                     /locus_tag="ACHLAM_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO7853403.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAVDAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(16697..17329)
                     /gene="traW"
                     /locus_tag="ACHLAM_001872"
     CDS             complement(16697..17329)
                     /gene="traW"
                     /locus_tag="ACHLAM_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO7853404.1"
                     /translation="MKYRGLIALLIWGQSVSAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(17326..17712)
                     /gene="trbI"
                     /locus_tag="ACHLAM_001873"
     CDS             complement(17326..17712)
                     /gene="trbI"
                     /locus_tag="ACHLAM_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO7853405.1"
                     /translation="MTTTQKTTDVTARQRSHWWWTVPGCLAMVLLNAAVSYAIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(17709..20339)
                     /gene="traC"
                     /locus_tag="ACHLAM_001874"
     CDS             complement(17709..20339)
                     /gene="traC"
                     /locus_tag="ACHLAM_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO7853406.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGLNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFRQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERIRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRQEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(20464..20811)
                     /locus_tag="ACHLAM_001875"
     CDS             complement(20464..20811)
                     /locus_tag="ACHLAM_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853407.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPVYIVYLAQQPATPEQVTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEARRVRENELNKIREKQ
                     LQALSDADSAKEH"
     gene            complement(20839..21057)
                     /locus_tag="ACHLAM_001876"
     CDS             complement(20839..21057)
                     /locus_tag="ACHLAM_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853408.1"
                     /translation="MHTFIFLVLMKNVGGGGIWNITPVPNTDVCVQILRRIEARGGRA
                     LGNTGECIEVKTNEPLNNVMETLNGGGQ"
     gene            complement(21137..21610)
                     /locus_tag="ACHLAM_001877"
     CDS             complement(21137..21610)
                     /locus_tag="ACHLAM_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853409.1"
                     /translation="MHKSETEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLTCCGAVCFFIYFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQHEQDTIRKFTEENKSE"
     gene            complement(21621..22016)
                     /locus_tag="ACHLAM_001878"
     CDS             complement(21621..22016)
                     /locus_tag="ACHLAM_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853410.1"
                     /translation="MHKSETEKIDFILDEHERIFRKQGSLCMALGKVFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDIPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQER"
     gene            complement(22009..22230)
                     /gene="traR"
                     /locus_tag="ACHLAM_001879"
     CDS             complement(22009..22230)
                     /gene="traR"
                     /locus_tag="ACHLAM_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO7853411.1"
                     /translation="MSDEVDEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQEKQRKHYA"
     gene            complement(22365..22880)
                     /gene="traV"
                     /locus_tag="ACHLAM_001880"
     CDS             complement(22365..22880)
                     /gene="traV"
                     /locus_tag="ACHLAM_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO7853412.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLADGNFRTTPVQAVASTTPSRSRPAVTALPEQKLLAPR
                     PLFTAAREVKTIVPVSSVVPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQTSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22877..23128)
                     /gene="trbG"
                     /locus_tag="ACHLAM_001881"
     CDS             complement(22877..23128)
                     /gene="trbG"
                     /locus_tag="ACHLAM_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO7853413.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFMNCDEKGEGQ"
     gene            complement(23169..23441)
                     /locus_tag="ACHLAM_001882"
     CDS             complement(23169..23441)
                     /locus_tag="ACHLAM_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO7853414.1"
                     /translation="MNMRNINVITARAVPGKTVSDDFMHAVLSNCTTKIVLPALEKFS
                     SESLPHNFNMAAVGVMKKGMSMRLFSSPGHIVVAGQSGGGKSVPAK"
     gene            complement(23428..24015)
                     /gene="traP"
                     /locus_tag="ACHLAM_001883"
     CDS             complement(23428..24015)
                     /gene="traP"
                     /locus_tag="ACHLAM_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO7853415.1"
                     /translation="MANNTSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEINFVRQTATAGQFPVSECRFSSSDLPGAEEKEICHYRNAG
                     ATEYARETDRSLMQLVTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVGTADERLRE
                     VLAEDASLPGKIRKCHVYFPDNRTNRNNGDKNEHA"
     gene            complement(24005..25435)
                     /gene="traB"
                     /locus_tag="ACHLAM_001884"
     CDS             complement(24005..25435)
                     /gene="traB"
                     /locus_tag="ACHLAM_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO7853416.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVLNKQRGDD
                     QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPEGEPQPGNAPVAFPP
                     QGAGAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKTFSYDAGTKQGPSLPYIPSG
                     SFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQAEESNPGSPVPNPPSAESHLNGLNTD
                     QMLKQLGDLNPQQFMSGGQEGGNDGK"
     gene            complement(25435..26163)
                     /gene="traK"
                     /locus_tag="ACHLAM_001885"
     CDS             complement(25435..26163)
                     /gene="traK"
                     /locus_tag="ACHLAM_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO7853417.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGKMPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLAAARMDVYVIRDGEGN"
     gene            complement(26150..26716)
                     /gene="traE"
                     /locus_tag="ACHLAM_001886"
     CDS             complement(26150..26716)
                     /gene="traE"
                     /locus_tag="ACHLAM_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO7853418.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(26738..27049)
                     /gene="traL"
                     /locus_tag="ACHLAM_001887"
     CDS             complement(26738..27049)
                     /gene="traL"
                     /locus_tag="ACHLAM_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO7853419.1"
                     /translation="MSGDENKIKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIET
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(27064..27423)
                     /gene="traA"
                     /locus_tag="ACHLAM_001888"
     CDS             complement(27064..27423)
                     /gene="traA"
                     /locus_tag="ACHLAM_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO7853420.1"
                     /translation="MNAILSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPAAVLMMF
                     FPQMALAAGGQDLMQSGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMTVVGL"
     gene            complement(27456..27683)
                     /gene="traY"
                     /locus_tag="ACHLAM_001889"
     CDS             complement(27456..27683)
                     /gene="traY"
                     /locus_tag="ACHLAM_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO7853421.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLSRFPDFYNLDAIKEGTGETDSIIKDL"
BASE COUNT         6852 a         7543 c         6296 g         6995 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaaacaac ctccgggaca tccggttcga agaggctggc
       61 catctgacga ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc
      121 cgggttgatg tcacgcggaa taaactccgg agcaaccttc ggtcgtgcct gatatttcag
      181 ggagagtttt actgccggat acggtccggg cagggtgaca taacaggtca gatccggcag
      241 agactgaatg tcggaataac tgaccagcgt ctgacgctcc atatccttac cggtcgatac
      301 cccgtcacgt accggatcag caccgtagga atactgctcg ctggctttca ggtgctcttt
      361 ctcaccaatt tcaccggcag cgaactctgc aatcttatgg ctcggggaac ggaaaaaggc
      421 acgggtgttc atgacatcaa acagcgtggc cgccgctttc tccccgtaga tatcttccag
      481 ctgggcatag gactggatac cgaacacata acagccgccg aacttacggg cttccggcag
      541 gatctccacc aggtccggca gtttgtgtaa cgtgggtaac tcatcacaga aaaaccacac
      601 acgacggtta cggttttctc ccattgccag caggccacga atggcaatgg acagccacat
      661 ggagatcacc ggcttcaggg aggcatgcgt gtcggcattc gacgagatga acagccagcc
      721 gtttttctga tcttcccgga caccacgcat ccagtcacga atagtgaagg actcaccgct
      781 gtgctcaatc ccctgcaggt aacggatggc ttttacgtag ttggtcagca cagcacggat
      841 ggaaatcgcc gttttctcga ttttctcttc caccaggttg gccgccggtg aattacgcag
      901 gaaggtacgc agtttttcaa ttttgatgga aagcagtgta tccaccagtt tgctgtagct
      961 gcggttgggg tcattacgca tcaggtacgc cgcttccgca aaaatggtac gtcctgaacc
     1021 ctgccagaac gggtcctctt ttgtgcccat cgggatcagc gtgttcgcgg tattatcaaa
     1081 atccggctgt gtcaggcact ccttccacaa atcccaggcg gcacagcggg catccagcgg
     1141 attcaggatt ttatcgatgg agggatcata gtaactttta acaaattccc ctgaacggtc
     1201 ataaatcacc accatatcac cgcgctgacg ggcgtagttg gccagacgac ggataacttc
     1261 cgacttaccg gccccgaccg tgccgtgcag acagaagttc tgaatttcag aatcccggat
     1321 aatcggcaga tcgccaatcc ggatatcgga atccttgccg tcttttttca gcatccgggc
     1381 aacgtctttc ggattgtctg tcagctgacg accacctgtg acttcatttt cgctctgttg
     1441 tttaccctga cgccccagaa tccaggagac aacaaagaag gtgatcaggc agataaccag
     1501 ggccacgaca gaagccagaa cgaatgccga ccataactgc tcaccgcacc agatcatata
     1561 tttatcatgc agtacctgag ccgcgttcat ccggaaggtt ttgccgtaat actggatctc
     1621 atagaccggc tgggacctga ttaaatcccg catgccttcc agcgtggtac accaccagta
     1681 aatacagccg ttcacaaacg tctgccagct tatttttacc cataaaacca gaccaacgag
     1741 tatccagaaa aaaataaaca ggcagtaaag catgatattg gcgatctggc tgaacatgcg
     1801 gatacgcatg gacgcaatct gaccgccctg ggtcatatcc tttgcgttaa aactcatgat
     1861 gaaactccgg taaagtcagg aacagagcag aaaaagagag gtgtggaata cattcacaga
     1921 acaaaccatg tataccctgt ataccggtgg tgatagccgc ctgctggtct gccggaagaa
     1981 actttttcca gcacgatatc agtcaggcgc aatatttcag accagtcact ctcgtgtgaa
     2041 tactggatct gaacaaaaag gcgtacccgt tcacgataat ccagagccgt gcccggttca
     2101 taaaagtggc cactgtcagg agaaacacgg gacgttttat tgatgcgatc gtatttatcg
     2161 gcatgagtct gtgacgacag agaccaggta atattttctt cctgctcacc atcaatacgg
     2221 ctactcccgg gcgtccaggc aatttcctgc gcactggtca cgatgatctg tctggagact
     2281 tctgccagct ttttgggggt acagaatacc ggggccagca cacacaggga caacaaatta
     2341 cagcggccct cttccggctt aagcggattg ctcccggatt tctcaggaaa agatattttt
     2401 gtcagtattt cagttaattc cttatgcctg agagaattaa taaagtacat gacaagcatc
     2461 atgagaaaca atataacaat aagccctccg ataaccacta aaaggtcagc atcaatatta
     2521 ttgctgtaca gataatcttt tgtggtcgct gtattatcct gtatcaccgg ggagtattca
     2581 gttacaggct tctcagtaaa ttgattattt tcaccgacat attcagcgat attgtctgat
     2641 gtcataatga ctttctccgg aagcttccgg cttcgccggc aacacttcac ggtcgcacaa
     2701 agaatttgca caaccgttca gtgttctcat atttatttcg gcaacgccgg gaagtgacaa
     2761 aatttatttt gttactttcc ggggttggac aacgagcaac gcgaggcgtc agctattttc
     2821 ttctgcgacg tccggtcgga tattcactca caggaagata ttctgttgcg cttacgccga
     2881 tctcatatgc agagaacggt tccagtgctg tcaggtatct ctggcaggct atggccagtt
     2941 cagaatctcc ggttgcatca gcaaaccggt aaagttcttt taataactcc cgacatacat
     3001 gctcaggata cttttcactg tcatagccca gcagaaagag tacctgcgag agacgagatt
     3061 ctgcacggtt catgtattca tccggggcag gaatatcggc ccggtacaat ctgtccgggc
     3121 cggatgggta atttacagga tgttcgcgat ggatttagcc agctggtctt ccagtacagg
     3181 cttcgcttct tcaaatttca ggttaacctt gttggcattg gataccacac gggtctggta
     3241 tttatgctga tttccggttg atgtgctggt ctgcactttt gcaccggagg ttccctgacg
     3301 gagagctgca acattatcag ttcggacttc agatgttgtc cgctctgcaa tctgcacatc
     3361 cgtgatcatg gtgtagttga tatcctccac cattgcatcg gcagccatac caatcaaccc
     3421 tgtggcaatc cctacaccca gcgccgcacc ggcagagctg gagttatatc ctgtgatgcc
     3481 ggtgcccaat gctgcaccgg caatggcacc ttcataaccg ctgttcagcc aggtctggga
     3541 ctcccgcaaa tccattttgt ctgccttcag gacattggcc tgaatccagt agtatgcctt
     3601 gtcaggtgag ctaacaacct ggtagccttt ggctttaacg gcatcactga tttttgcctg
     3661 caatccgctc atatccttgt cagatgtgtt tttgatttgc aggaaaacgg tgcgttcact
     3721 ggaaggctcc agccagatgg tttcactcat ctgagttttg acttcaagat tacgcttctt
     3781 gattgctgtg ctcatcgcac cacaccctga aaggaccaga gtggaactga ccagggtaac
     3841 tatcatcaat tttttcattt tcatcaatgt tccttcatat tgaataaaac accataatat
     3901 acaaattaag attcactcat catatcgttt tgaactataa ccatataaat taccaccgat
     3961 atccacacca atacctaaca taggtaaccc tgttgcagga ttagtacgat gaaaatccat
     4021 attatcaaaa tccagatgca aatcttcatt agttttttca tttacctgag attgcatcca
     4081 ttctcttaca cttacacagc cacaaactcg ccttaattcg tatagtgtac caatgataaa
     4141 agtcatcagg cttaataacg gagagaataa ggaagatact ggcatcagta tataccaaac
     4201 gcctttgctg aatttaaact catccgacac atgccgtttt tttcgtattt gagaaaacaa
     4261 ccatataaac aatccctgcc atagtacaat aaagcacagg ataaaaactg tttcagcgct
     4321 cccccaatca gccagataac aggcaatgag ctgtatcacc gttatcagag caatatgagc
     4381 tgaatttttc atcatcatct cccccataca atattattgc ttgtgcttat cctgatactc
     4441 ctttgctctt ttcatcagtt cctgtgggct gtcagcacca ggcattcttt cctgagccga
     4501 cttttcttca ttatatttat tatcctgaga caatacctct gttttatggt gattttgcag
     4561 ttcagaatac tgccctctta caatattttc ctcaccgagg atgctgttct gagtatcccc
     4621 gatattgcct cgatattctg tgaccatatt atcaacctgc tgcttcacat cgttacggat
     4681 attactgtcc tgcgttcttt gctcaataat accctgatgc ccctgatggt ctgcaataat
     4741 atcctggctg ccaccgcccg aaggtacgct ctccattcct ttaccgatat cgccacggga
     4801 ttcacgccag gcgttatcaa caccaggctg tacctgttcc tgaacaaaag accaggccat
     4861 tgcccgacga cgttctgcaa tctccggtga actggtgttt gtcagaatag cttctgcatc
     4921 ctgcggcgcg tgtttcatca catactgtgc aaactgttgt gacaggtctt cactcatctg
     4981 cccactcatg ctttcagtac gggaggccat ttcagcatat tcatggctgc gggtcatatt
     5041 cgtcgtgtac tggtcgtaac tctgtttcgc tgagttcagg gctgcagaca gctggtccac
     5101 cctggaatcg gcattattgt cagtatggct tccggattca ctgaccttgc gactggtaaa
     5161 gtaatcgctg gcctctttaa agtcttgtgt agctctggca tcgatatcat gacgagcatt
     5221 atggctcgac tgagtattac tacttgccgt atgcgcatca ttatcttccc aatcaatacc
     5281 agcccgacca ccacctctga cacctgcgcc accaccaaca ccaagaattt taggtttaat
     5341 cccccattca gcatgagcat ctccatacat cccggcagaa gctctcgtac ttctggatgc
     5401 cagctcctga gtcgcctgct cattactgat attatgcgcc ttcgcatagc tttccactgc
     5461 gctgcgcata cggctggcca tcatggagtc ctgcgcgctc atcgtactgt cagcgccact
     5521 ggtgacagaa tcactacttc cccggttaga gccaaactgg ctgagcgtgt tccaggcact
     5581 ggcaatactg ctgctgaaac catgcagggc actttccgcc ttattcatcg actcccgggc
     5641 catttcctgt tgcgctgctg caatctgacg cgttgcatta ataccaaccg gcaaacggga
     5701 cattgcccca ctggcatcca tcaccatact gccgtcacgg gtctgcgtgg cggttgctcc
     5761 actaccggtc tggtgcgtca tctgaccaaa tgacgtggta ctgttggtgc tccagctgaa
     5821 tccgttcacg ttattcgtct gcatgttgcc gtaggagtaa ttaccgtcaa ccacactgcc
     5881 tgccgcactg gcggttgacg tgatggaaga agacgcaaag tggctgtata cgctggaaaa
     5941 tccggcacca aggcctctta ccatcatcca cgaaagagga gggatcatcg ctgagagata
     6001 acctgcggtt gatgccaggt cggagtattt cagctgtatc tgagagactt ccgacaacac
     6061 caccggggca ccgttctgct tcgcatagaa tgtcatggca ctgttcagaa tggcgtacag
     6121 cagaggccat gtctgcagcc acataagggc aaacacatag cccttcagca ccggcagtgt
     6181 cattttatta aatgcagcag ccagtaccag aagcgggaaa ataccaatgg ttattccggt
     6241 cagaatggtc tggaccatcg gcaggtttcg cattattaca tgtccgacag agacgtgcgc
     6301 cagacgctgt ttctccattg atgaggtggt ggccagactc accaggctgg ccgtatcacc
     6361 gttacgggcc gcattactgg tgataccctc tttcagtgca tttattgtca cattcttacg
     6421 cataatctgg ctggccgact ggctggcacc gtagaaatag ctgtaactgt cactgagcag
     6481 ttccctgaat aacaggtcag ggtccggtct gccgccaaat aactgctgca cataataatg
     6541 ccaggtcttg cctccggtct ttgtatcaag attcagctta tccttcagtc cgacggatgc
     6601 gtccttacag gacacaaacg gctctgagct ttgcagaaaa ctgtaattat tattcggcac
     6661 ctgacgtaaa gggctgggct gggaaaaaat caccatatag gggtcatcag ccaccatcag
     6721 gtcttccagc gtgtatttgt ggttcaggta aatatcaccc agcacgcagt tctgaacgta
     6781 gtcctgaaac aggttgataa tttcgggatt ccgggagaga aaatcggtgc ttttcactat
     6841 cagattcgcc ccgaacagca tccctgtctt gctgtaggtg gcgctgtccg gctgagcgaa
     6901 aaccatctca tagccggcca ccattgcatg cccgatacgg gttgtcagcg ataatggcat
     6961 cgccagcccg accggtacat tgtccacacg atgaacctga accaggtcac tgttatcaat
     7021 aatctgcacc gatgtgcgga cattaatcag cagactgata agcacaaaca cggcaaccca
     7081 tcccagcaaa tccatcacgt tatgtcgctg tacccacata aacgccacag caacaacaga
     7141 cagggtgagc gcaatttttt cgatggaatc ccacgtccgg gtactcataa aggcagcgat
     7201 ggcattcagg ttgttccgta accattcccc accggcgatg acatagacct cgttcactgt
     7261 acgcctcccc cgaagtgata attattctgg taacggctga gcatacgggc agacaactgc
     7321 tggcgcatgt agctcatctg acggtcaacg accagcaatg catcctgctg gacctgcacc
     7381 tggctctgga aaatggcaat ctgttgctgc gcctgattaa ggttatcgag cacactgtcc
     7441 aggaccgcct gcgggtagtt cccggtggaa atcatcgccc gggcctgctg aagcagttcc
     7501 tgaatgtact ggagcatgat gtcgtaaccg atatagtccg tcagctggta gagaacactg
     7561 ttggatacgc ccagcatcag tgggtcaacc agatatctga aaacaggaat ggtggtgctc
     7621 tgaatgaagg ttttttcgtt ctcagccagc ggagaatcac tgagcgcctt gttctgaata
     7681 ctggtgagca gtttcgctat ctgtgacttg agcgccctgt ccttgctgat ggtcacattc
     7741 gcatcggcca ccactttgag gcacttatcc gaatcattgc agtgataaat tttcgcggtg
     7801 cccccttcca taatcgcccg gacaatatcg cggtcagtgg ttcgtgcagg aagaggggta
     7861 atctcactgt cttcaccaaa aatcagcgta ccagtgagcg tcataatgaa ctctttcagc
     7921 tctttgttac catcaagcat cctgtttttg gacagcgagt cccagatgat attgatgttt
     7981 ttcagtaccc gctccttgtc cttatcgctg gccttgtcac gcaccgcaga agactgccct
     8041 ccgaccgtgc acccctgacg ggaagcggcc cagtcagcga agatattgct ctcacctgca
     8101 atatcctggc agaccttctg ctgcgactcc tgcgtctgag gaaacaaccc gccaactatc
     8161 ccctgggcca cctgacagct actgaggttc atactgttaa tgtcattcgc catcttctgc
     8221 aggaagtctt tcgcggtttt gatttcgggc accgttgtct gcagggcaag gtcaaaaaag
     8281 taccccatcg cgttactcat aatctgctta acaaaacgct gcagctgctc gccattaata
     8341 aaactgaatg agccgagata ggcatcgatg ccaccacacc ccgcattgat gtcgggcagt
     8401 gtcatggaga taagctggat atttctgacc tgattacggg catacagaga accgccatag
     8461 gcatacccgg cagcctgccc ctgccagact ccggcctggg tggtattggt ggcaaagccc
     8521 agcttgttga aaaactggtt catgtcgctg ttcacatccg ctgacgcagc aggtgtgacc
     8581 gtcagtaaag cggcacaaag aaccagaaga ggtttaatgc gaagcattat tgccaccctc
     8641 ccttaatttg ttctcccggc gcatacggcg tttcagcagg aatatcatga tattcccgaa
     8701 tattaattcg aacattgggg acagaaaaaa tgtaacaaga aaccctgccg caagcgccaa
     8761 taacgtacac ggaatggcat acatcaggat attccacata tcacgtacgt tatttaattc
     8821 agggtcagcc ttccacgaat caatacaggc atcaaaaata acattcatta ctgtccataa
     8881 aagcaatccg gcaacaaaga aaaccacaaa aagccagaag tgttttatgg cattatcaca
     8941 accacgctca atatcccgtt taatttttct gattttgaat tcctgattct ttttgttttc
     9001 tttcattata agtatcctct gatatttctg atgattcatt cagacaggtt atgcggctgt
     9061 aatatatttc agacaccaaa acccatccca caattgctcc cacaaaaaca caacagaaac
     9121 agaaaaggta agacacagcg aaacgacaat ttctacaggg ctggcgggat aaaaaagtcc
     9181 ctgacggata aaggtgatat tcaggctgac gatgattccc gtcgcgatgc cggatattcc
     9241 ggcaataagt aacaccacct gcttatttcg cacctttttt tcctccgtac atctgcagaa
     9301 cggtatccat ccgcgccata aaaccggcag catccgatgc gccctgtaaa agcggcagcg
     9361 cttcaagcgt attgacgtta accaggaagg tggtcggtgt ggccaccggg atattcggga
     9421 agaatgtttt cagtacatcc ggtggtgcag gtaacgcctg cgggaaggcc gtatcaccct
     9481 gaccatccag ggtataggga aatacggaaa atccgtactg cagcgccagt tgtttcagta
     9541 ccgggtcaaa ctggtgacag tacggacatc gcccctgcat gaacagcacc actttccagt
     9601 cagcaagatt gacctgagtt ccgttgctga ggcggaacca gcgggttgct gctttctcct
     9661 gtcgcgcctg tgcatctgct gccggctgag ttgtccgtgc ggccattccc tccggatgcc
     9721 agagtcgctc gatttccgca cgtgtggatg cctgtgtaca gctggccaga aaaagcagga
     9781 caacggacag cgatttaacg ggaaacatgt tcatcatcat tatccttccg ttctctcagt
     9841 aacaactgct gtctgacttc cagtgcctca cgctcttcct gactgacacg ggcaatccac
     9901 gtatcaagca cacgatatgc tccccacagg accagaacag ccgaaataac ctgagccagg
     9961 atatatgcca tccagggttt gctgttaacc agacgggcaa caatatggaa ccagacaccc
    10021 agggaaaata cccacattcc cgtaatatca agtcggggta aagagaattt atatttgcac
    10081 ataaatatat cctgtttgta atatttgcaa cactcagcag gatgacaaat aaaaacctga
    10141 ctgattatta ttccgacata ctcatttcat tttcacattg aatatacaaa aaacagagaa
    10201 acacccataa aacaaacaga gcaacataaa atgctgccgt cattaaaaaa tcatccagaa
    10261 atctgagaag ccagatacct gctgcaatga ttagggcaag cacagacaag tacaataacc
    10321 tgctgttacg ctcgtaaata aaataaagca ttaccattac aacagacagc gccacataca
    10381 ttaccccggt cacactcaga attaacacat tcatcagcca gctataatag cgattcatac
    10441 tgacatccgg atctggaaaa ctcataaaag ctaataaggt cagtaaaata accgcaacac
    10501 cagacgcgat taaccatctt tcagaaatac tacaattttc tttatccata cagaataaaa
    10561 ccatacttaa caaacaacat gaactaaaag ccattcacca tcaggtgggg ggacagaaaa
    10621 gagttgtctc tgtttgtctt ttactccgta aatgagttta tttacatatt aacagagcat
    10681 tatttcatta tggtttctga tggctggagt aaccagtaga atataatcat tcctgcacca
    10741 gaaaacactg cggcaacagc aagccacaaa cgcttatgct caccttctga tatgcctgaa
    10801 aaatatcctg tatggagtag cagcagcaac catgacaaca ctcctgccag acccagcagg
    10861 gcaggaggga tatgccacag ttcatatttc ataataacaa taccttctgc catcatcatg
    10921 gacagggtgc aaacaacaaa acagtcccat gaaaaaaaac gcctgtcagc ggcaaggaaa
    10981 atcactgtca gtgacatcac tgtcacctgg gccagcaata aaggccataa agaaataaaa
    11041 ctcatttaaa cgacctcttt aaaaattcgg tttaaagtct tcagaaacgt tcaggaactg
    11101 ttttgccagg tcgtcctgcg aaataaagcc gtatgataac ggacgaacac tgccctgttt
    11161 cgggtcaacc agcatcatag ccgggaaata tttcacgccg aggcgctgcg cctgcccctg
    11221 gtcagtccgg gaatccggca atagtggatt aatcacgcca tccacggaaa cgggaataac
    11281 actcagacca tacgtatccc ggaagccatt aatgacctgc gccagctgcc cgtcgatggg
    11341 gtcctgcccc cggtaaaaaa acataatgcc gtaatgctct gccagtctgg caatcgcctg
    11401 tcgctgctgc gcctggtctg ctgccagctg gttccgtacc gtgccgttgt aatggctgta
    11461 ctgcaggtta tagtccagtt caggatgtgc cagcatggcc tttttggcgc tcatggtgaa
    11521 aagcccggcc tgctgagtcc agtaattctg cagccggaaa tatttcacaa aattatccac
    11581 gccgggatac agaatggctt cgtacagcgc ccgctttgtt gccgtctgca gcgcggtcag
    11641 tttctgcata atatccggct cctgacggga agcggctggt actggtttat tttctttttc
    11701 cttcggattt attttctcgt tataccactg ccagcctgca tctttccctg acgccggaaa
    11761 aataaagcag cagagtagca gtggcagtaa tgctttattc atcttcattc ctttcatcac
    11821 tttttctttc atcagctttc tgcaggtcag tcagggcaaa aaagcaaaaa tacagaataa
    11881 acaggagcac accggagtaa aggataacga aaacagcagc aagcaccccg cctgccgtgg
    11941 tttcttttac cgtatcccat gaccagataa ttaccgggga aatgacaatt gctgtaataa
    12001 caagccggat aagaaaatca acaagacgat tcgacgtgaa tatgactttc attattttcc
    12061 cgaccttact gccccacctg tttcagctga ccggcaatct gctctttcac tttttcggtc
    12121 agcgcaccat tatccgggat tttctggtta ttcatcaggt cttcgtagaa gttagtgaag
    12181 tccagctggt cgaacttaat acgctgcagt tcatccacgg ttattccccg gcagtcagga
    12241 ctttttgcgc tgccaaaccc gatacgcaac tgcccgttac gcccctgctg ctggactatc
    12301 tgcgccagct tcgaatcaaa ctggcagtaa ctgcgctttt tctgcagaca gacacccagc
    12361 actttccggg agcagaactc gcccacactc accgtcagtt tgttgtcctt tgcctttccc
    12421 agcgcctttt cttcgctgtt gcacttcgcc agacccacat cctgacccca gccaccatcc
    12481 ttacagcagt tactgaagcc ggctgcggcc ttacggcaga atttcgcctc gcccgtgaaa
    12541 gccctgacat ccacaccatt cagggcggca acatccttac ctgcggcggc aagcgcagcc
    12601 agctgtgata ctgcctcacc aaaatcactg ctttttccgc tctgcgcctt atcgcattca
    12661 ccgtcaaggc agaagacatc accaccacag accatcactt ttcctgatgt tctggactcg
    12721 caggagtacg tggcatactc atgcagacag cttccctcat cggaataaaa cgcgcactga
    12781 cgtgaggcca gcgtacaggc cggattatcg acatatttct gacaggtgcc gttatctgcg
    12841 gactgtgtga catatttatc ccggtatgcc cagcacgcct gtgtgatgct ccaggtttta
    12901 ccttccatca cacctgtttt cgtccccccc ggctctgaac actcagtacc tttcagcacg
    12961 ccatcccctt tattaaacgg acaactctcc acccactcca cccgtggaac ccattcttta
    13021 tcacggacct tcatcctgag tttcagcgta aaggtggaag caccactgac aagcgattca
    13081 tagaccatcc tgtcaccatg tccgtgcggg aggcaattac cgtttgcagt acagccacta
    13141 ccggtcagaa cctgcccctg tgtgacagaa aatccggagg gcacaggtat ggtgaaagtc
    13201 ccgctctgtc ctgtaaccag ttgcacatta aaggctgtgt tcataaagtc gtaacgggag
    13261 ttaaggaaat atagccctgc atgagccgac agcgaggcac tttccactgt cccggcaaca
    13321 ggtacggtaa cgctgaacac cagtttcaga ccgttcatac tgaaccggaa ctgtgagtgc
    13381 ggtatcgtca cctcacgata ttcatccgtg tatttccagt ctcctatgat cctggctgtc
    13441 ctggaacaat attcctccac catcgtgtca cgttcacagg tatagtttgt gaattcactg
    13501 cggtttatct gctgcgcact gcactgctgt ccggtatttc ccacgatact gtcagcccgg
    13561 ttcaccacat ccctgccggt ctgaataaac ggcgcatccg gtgacagaat gtctttcggc
    13621 ttgttcatga atgactctgt gatggttttg ccggtttcac tggttgccca ttgtgtggtg
    13681 ccgttatttt tcagaccgcc atccccgccg gcagtcaccc caccgtaata ctgcgtctca
    13741 tccgggttcg cgttataccc ggggatactc tcctgcggat taaaactctg aatactgcca
    13801 ataccctgcc cctggatctg acgggcaaaa tcggaacctg cccggtaatc actgttgctg
    13861 tcggcaaata cagaaaagct caggaaaaaa acaattgcac aaatattgcg cggtcttccg
    13921 gagtcattaa cattcagaga cctgaaaaag gtatttatct tttctttcat aacctgatcc
    13981 atactggaga atgaagtgat gtcatctcaa cttcagaacg gtgttgattt tggtgtatgg
    14041 agcttcctgg cgtaccggga ttactactca gataacgact tcagtaacaa tatcagtgtc
    14101 ctgaagtctg cacgcagtga acgacgacag taccggtgat accagcaatg cggaccttcc
    14161 ggctgaagcc ggaaacagag ctgaaggtca ctgttaaaag cccccttcac cgaccatccc
    14221 catgccagac gtggtattgc tgattcagca tcctgccgca gtcgccgttc aagaagggca
    14281 gccattttta ccggacagac aaaggcaatg taaggcggca gctttgtaac agggaaacca
    14341 tgtccctgac aggaggcata tgtacggaat ccgtgaacgt tcatcgcggc caccagtgca
    14401 cggatgtccg ggtcaatccg gtaatacccg gcaggtaaac tcattgcggc ttatccccgg
    14461 tgccgtccag cagtccctga gccacctgcc ggcagtctcc cgtggcggcc actttttcca
    14521 gcgcctgcct gacgcgcaga tttccccgga tgatgtcgtg cccctggctg cagaacacca
    14581 ccagggccgg cacgctgcgg atgccgtact gtgaaaacaa cgtcgggtcg atctgaacac
    14641 catcggtcgc gccatctttt accagagaca acacggcttc agcggtagtc ttcaggtcat
    14701 tgttcaccat gccccgcagt gtggccggaa tgccgtagtg ccgggtttct cccagcatcc
    14761 gtttcagtcc ctcttcggga atggagaacg acacaaaata cacggccccc tgctgtggtt
    14821 tgtctttctg agcagcctgc tgtttacgga ccagttcatc gagaaactgg ttatcggcat
    14881 gctgaagggg attcgccctg acctgctgtt ctgcccatgc ctgcagttgt gtgtccggat
    14941 tttcacgcaa ctgctgactc agtgtttcct gctgcttcag gaactggcga ttttcgggcg
    15001 tattcacgtt ttccgaagcg tgggcagaaa taaacagtcc tgcagtcagg gcgaataaaa
    15061 tgtatatttt tttgttcata taattctcct gcaaacaggc agtaattaat cgctacaacc
    15121 aacacctgat atcaacaacc agattctcac ggtatttcat ggattattaa ttccgggatt
    15181 acgcgcccgg tcgcgttgtg gtaaaatatt tcagcctgat atatcgattg caagagatga
    15241 caggctaaaa acataccaac aaggagttac aatgaataat agcacatcag aagaccgccc
    15301 tgatttttca ttcagttata tggacaatca gagagcagat gaaataaagg ctttaaaaat
    15361 attattattc agcatcatca gaaatatgcc tcatgataca gccatcagta ttattaataa
    15421 catcgtaaat actgataata atgaggcgct caatagattg tgtctggaga taactgacgc
    15481 catgttaaac acaacgaaaa tcgttgatta acttctttct ccatttatct tttttctgag
    15541 actggcggca attaattgcc agtctctgta ttgctctgct gttatttcct ttttctctat
    15601 actgtaagtg acctggaatg cagggggagc ttcatcctga tgcaagggaa gctgcgggcc
    15661 acattgtttc tgttcttttt ccatcatctg tccttttctt gtcagagtta taaaaacaca
    15721 cagttacgtt tacgccacat caggtaaccg aagtttttct ttgtgttggg cggatttttt
    15781 cccgtctccc agcgcatcac actgcgtccg aacggatggc actgtccgct gtccgggtac
    15841 atattcacca tctgataacg ccagcgctct ttgggaagta tgggcgacgg atattcataa
    15901 cagacagcca catccacgcc gatgctgttc attatcattc cctgacggtg gagcttaaac
    15961 gccatccgtt cactgaccag cagggatgac tgcaacggac tggattcatt gctcacccag
    16021 ccattaaacg gatacatact gccctgcgaa ccggcacacc agaacaggat atccagcggc
    16081 ttgttaaagg cactggcaat ggcatccacc gcacaggctc cctgtgctat cggatttgca
    16141 aagatgaccg cttccgggtt gagaatggtg gtcagactgc tgtctgtcca ggtagggtca
    16201 atttctgaga tgtacccgat atccatatcg ccgcccatca gacatcctgc tgaggtgata
    16261 atactcagcc agtatgtcag cgggtatttg taccagtgaa catggtaaaa tgccccgttc
    16321 acctgcttgt cgtctttttt ggccgttccc tgcccggttt tccccagctc cgggattgtt
    16381 attccaagat ttaccataca tcccggactg cgggtcacat ccgtcagtgc catcggctcc
    16441 cagtaaccaa tagccagccc gatacgttta aatacaggcg gtggcgcagg acagaactgg
    16501 atcggcattg acgggtttat tgtatccggc accttcccct gactcacttt tgtgctgccc
    16561 agcgacagtg gaaaaatgca gttccagcag atatccgtga tcgggttaac aaaccgccct
    16621 tcacaggcag cgtccgctga cgctgcggga acatggccag tcagcagcaa aaccacacac
    16681 aaccacatac accgtttcat ttccctccct ccccgaccgg aataaactcc accttcagga
    16741 aacgatcgcc actgacagca ctcacccgtg ccggcacctg atcaatgccg agacgctggc
    16801 agatcacgcc gttctgatca aagtaaacac gactgtccag agccttctgc atctccggga
    16861 tactgccctg caccaggata attttgctct ccagtgtggg aggcgtctgg cgcttcatcc
    16921 attcaatctg ctcaggatca tcaccgttga tgaaatacag cgtctggttg aaaggcacat
    16981 actgcagcgg gttcatcacc tccccctggc gggcgaagat acgaccttcg ttatcccgga
    17041 tatccgcagc cagtctgaca gacgggtcaa acagccggct gctgtatttc tccgtccgac
    17101 cgataccggg cacggcagga ggccgcaggc tgttgcggat cacccgctcc ttaaacgcat
    17161 ccattttccg gcccatctca ccggactgct ccagtgccgt caggcgctgc atgatcaccg
    17221 tcagcatgtc cggctccttt accggccaca aatcccccca ggtgccaaga tcggctgcgg
    17281 acacactctg cccccatatc agcagggcaa tcagcccccg gtatttcatg gttctgccct
    17341 cattcgccgg gcaatatcct gctggatttc acgggtgata tccggtgccc cctgtaccac
    17401 ggctggcgac accagaatga cagcgtggtg cttctgctgc cacgtctgca gactggcctc
    17461 cagtgccgcg ttaaaacggt cagccagcgc cttagactgc gcttctgaca gttttttctg
    17521 gctggcactg tcaaaaaacg catccactgt ctgtttcatg ttgaaggcga cggttaccgg
    17581 cgtgttcagt ctgacaatgg cgtaactgac ggctgcgttc agtaacacca ttgccaggca
    17641 tcccggcacc gtccaccacc agtggctgcg ttgacgggct gtaacatcag tcgttttttg
    17701 cgttgtggtc atgcaacact cctgtatttc tcatgttctt ccagccaggc ctccagcgaa
    17761 gccatctccg ggcctgactt cttccacgcc agctgccaca ccgcctcatg aatgctcagg
    17821 ccctcctgac gtttctgctg cacaaactca aaatccgggc cgtcagagct gtacatggcg
    17881 cggcttaacg ggtccacaaa cagacggtgc caggaggaat ggttttccac ctgcagcagg
    17941 aaggaactga accactggtc tttggccgca ccaaacttgc caatcatgtc gcgctgcagt
    18001 ggcaggaact ggtccgggaa taactggtta tatttcgcga actccttcgc actctgcttg
    18061 aggataattt tgtaggagga gttaccccat gcggcacggg ccgcactgga ggccttgtca
    18121 gagtcaaagt cgacgatgtt ctgtgtgatg gtgatatagg caccggtatg acggcgggcg
    18181 gtacggtagc ctttctcaat aaattcaccg accttgtgat ttttgaagtc cagcagacgc
    18241 cagccttcat caataacgtt cagtttcttg aggttacgcg gcgtgcggta catccggttc
    18301 tcaatgtaaa tgataaggct gaacatcacc gcaaccagca gggacggacg gtcttccagt
    18361 ccgcccagct ccagcaccac cattttcgca tcatcccgca gggacggctc atcggagttg
    18421 aaatactgac cataagtacc gttagcagtg tactggtcaa gcagcacaat catttcgtcc
    18481 agacggctgc ggatggtcgg tgactcggca tactgctcgc tgtcactggc gtttttcagg
    18541 aaatccacca cgtcatcaat acgtgccctg ttctctttgg ccagccatga tgccctgact
    18601 gcctgcagca gcagaccttc atgcacttca tccaggttac cgttggggct ggccatcacc
    18661 gacaactggt cacggatacg ctccgctgac tggtcaatat cggtgatgtt cgcaaacggg
    18721 ttaaagcgca gggtttcacc atccagatat acgcccccca tgttctcaca cagggacttg
    18781 tagccatccc ccatgtcgaa caccacagca aagcccccgg agtccagcac gctgcggata
    18841 agtggctgta tcagcccggt cttaccggcc ccggatgttc cacagaccgc catgttgtag
    18901 ttggtgttat tcatcccccg gaagaaaata tcgataaacg ccagctggtt acggtaggtg
    18961 ggcgccagca gacctgccgg tgtcaggggg ttatccgcca ctaacggcat caggttggcc
    19021 acattaaagc tctctgcgcg ttgtaccact ccggcctctt tcagctgtct gaacagccct
    19081 ttcccggcca taaagggcag gcaggtcaga aaattgcgca tgtggttaaa gcgcggtgaa
    19141 atcagctcaa aaccgttttt acggaagctg ttcaggatgt cctgctccac ttccagcgcc
    19201 gtttcattgt tgtccttgca gaacgctgtg atgttgagga agtaggacac cacggaggac
    19261 tgacctgagc ccagccgctg acgcagttcc ccccactcct tcgcttcttt ctccacggac
    19321 ggaaaccatt tggcatagga ggtcttcgac tttttctcca ggtccatgta cttcaggttg
    19381 gcttcgctgt gggttttcac ctggtcttcc accaccaggg tcagcgtcag aatgaacggg
    19441 caggagatgg acagttccgg gttcagcagg ttgctgtagt tgtcggccat gttccacagg
    19501 aaggcgattt ccgggttacg ggccagatgg aaattcagga tgcgggccgt gctgttcctg
    19561 ccgttctcac gcagtcccag cgtcaggtaa tctgcccgga ccttcaggtc aaaactgtcc
    19621 tccacgcact gataattcaa atcagaatac gggtccagct gacgtctttt cgggtacagg
    19681 gagtccgggt tatggttaat catctccccg acaatatcga tgaaagcctg tgcatccacc
    19741 gtctgcgtgg tgatatatgc tccctgcaga gacgcccgga tgattttcac caggttttcc
    19801 atttccagaa tgtcggcccg gctttttttc ttcgagggag aacagtacga gataaacacc
    19861 cggtaatggc gcagggtcag aggcaggttc aacccttccg gcagcggaaa cagtgtttct
    19921 gcggctttca tataataggc ccgggtaatg gcgttaaacc gttcggcctg ttcacctgac
    19981 caggagaact cacgcagccc gtattcaatc ctgtcaccaa ccagctgact ggacatcaga
    20041 tggatacaca acggaatacc gcgcggcagt ttggtacgca gcatgtggtc gagcgcctcc
    20101 acaatggact cattcgcccc attgatggga atggcttcca gcataaagcc catcgtggtg
    20161 tcattcatga acagaccgga ttcctggttg taatcacggt acggcagcag acggctgaac
    20221 tgcgggaagc tcatttcgcc cagaacttca ttggcctttg cggattcgtc aggcagtttc
    20281 agtgctgtga cgagggagtt aaccgcctga gtgacggcct caagtgggtt attactcata
    20341 tgagtattcc ttagtacgtt taaaaaagga aaatatcagt ctgtggcgtg gatatatcac
    20401 gcagtgccat ttctgaaacc acagcacaaa taagagatgg aggcttttaa caggaaacca
    20461 ggattaatgt tcctttgctg aatcagcatc acttaatgcc tgaagttgtt tttcgcgtat
    20521 cttattgagc tcgttttctc ttacccgtcg cgcctctgcc atttcgtttc tttttctgca
    20581 ttctgatgcg attttatttg ccttaccaag tgttaaaggt tcagactgat aattaagtgt
    20641 ctcctgaaat gcttctgctg cacagggagt ttcctgtaaa atctctgtca cttgttctgg
    20701 tgttgcaggt tgttgcgcaa ggtaaacgat gtaaacagga atacatacaa tcatagccat
    20761 aacgccaaca aatataaaag ccagaacaga ttttaaatat atcgacttca tttcattttc
    20821 cttcgaagca gtaagcactc attgcccacc accattcagt gtttccatca cgttgttcaa
    20881 aggttcattt gttttaactt caatacactc ccctgtattt cctaaggccc tgcctccacg
    20941 cgcttctatt ctccgaagga tctgtacaca tacatccgta tttggcacag gtgtaatatt
    21001 ccagatacca ccaccgccaa catttttcat cagcacaaga aaaataaagg tatgtattct
    21061 ttattacccc cagtaaaata ttaatcactg aatattcttt cagttaactt atgaggttat
    21121 aaaacacagc agattttcat tcggatttat tctcttcagt aaacttccgg atggtatcct
    21181 gttcatgctg acgggtggca gactcctgca ttgcatccac tttctcctgc cagagacgcc
    21241 ggatatcctt ttcatcccga ccagtcagtt ttttcccgga cagcagacgg ttaagcagtt
    21301 cctgtctcgc atccggtacg tcttcagtca gacgcataag ctgaagcagg ctctgacggc
    21361 tgaggaccat gagtgattcc ggcaggaggg tgaagaagta aataaaaaag cataccgcac
    21421 cacagcaagt aaggaccgca aacaggattt tcagatatat accatcaacg ccgaacgcag
    21481 caataataca gggaatccat gcaatacacg cacaatacgc caggatgtta tctattcggt
    21541 tatgtctgcg atatatctgt tgatgttcat ccagaataga atctattttt tcagtttctg
    21601 atttatgcat agtgtttcct ctacctttcc tgccagagac gccggatatc ctgttcatcc
    21661 cgaccagtca gttttttacc ggacaacaaa cggttaagca gttcctgtct cacatctggt
    21721 atgtcttcag tcagacgcat aagccggagt aattcctgac gactgagtac catgcgtgat
    21781 tcctccggta cgctgtaata attaaaaata cgactgaata ccccacaaca gcaggcaacg
    21841 gacaggcaca gggatttcag atacaggcca gaaacacaga atgtggcaac accacaaaga
    21901 atagctataa ctaaaaaaac cttacccagt gccatacaca aagaaccctg ttttctgaat
    21961 atccgctcat gttcatccag aataaaatct attttttcag tttctgattt atgcatagtg
    22021 ttttctctgt ttttcctgat acgcctgaca ttcaacgcac aacgtcacac cgggaaatat
    22081 tttcctcctg gcttccggaa taggatttcc gcatgcctca cagagataaa caggaatacc
    22141 atgagcattt attttctggc gtatccggtt tattcctgtc atggtcagtt gttctgtcac
    22201 tgaatatgct tcatcgactt catcactcac ggaatattcc tcccgacatg ttctgacacg
    22261 ctaccgccca tcccttttca cgcacagggc actcgtttta caggcagatt ttgaatacag
    22321 aaagcccgcc ggcattgccg gctgatatac aacttcaggg cctgttaatt aatacgtggt
    22381 tttccccacg cagacggttt aataacaaaa aacacgctgg atgtctgatg ataaacatcc
    22441 tgattatcaa tatacggcgc tatccataat gccgccgtct gttcacccgt tcttagcgga
    22501 cgtgggggtg ttaccggcac aactgaactg accggaacaa tcgttttcac ttcccgggct
    22561 gcagtaaaca gcgggcgtgg tgccagcagt ttctgctccg gaagtgctgt cacagcaggc
    22621 ctgctgcggg acggcgttgt cgacgcaacg gcctgcactg gcgtggtccg gaagttcccg
    22681 tcagccaggc gcggcagtga tgccgcaacc ggctttgctt ctgaggactg ctccagtttt
    22741 ttggccttct cattggcctg ctccatcgtc atacaggtat cggatgtggt ggcatcacac
    22801 tcaaaatcgg agttcgttcc ggcacagccg gaaagtaata acgtccccag cagcggaata
    22861 aataaagaaa tctgtttcat tgtccctcac ctttctcatc gcagttcata aaatcattct
    22921 tcaaatccct gaccgtacag gtttttacaa tgtcaggatt ttcaaaaacc gtattccgtg
    22981 atgatttata aacatatgcc acaatgcgtt tcccccccgc atcaggttct ggtgcactga
    23041 cttcacataa cggaggtgtt attccggact catacagcac cggataattt atttttttga
    23101 cgctgccatc agaaaccagc ttattcatcg aagggttcct tatatttctg aataattctg
    23161 tcgattagtt atttcgccgg aacagatttt ccgccaccgg attgtccggc aaccacaata
    23221 tgccccggag aagaaaataa acgcatcgac attcccttct tcatcacgcc gacagcagcc
    23281 atattaaaat tgtgcggcaa tgattcagaa ctgaatttct ccagtgcagg aagaactatt
    23341 tttgtggtgc agttactgag aacagcatgc ataaaatcat ctgatacggt tttacccggc
    23401 acagcgcggg ctgtaataac attaatatta cgcatgttca tttttatctc cgttgtttct
    23461 gtttgttctg ttatcaggga aataaacatg gcatttcctg atttttcccg gcagggaagc
    23521 atcttcagca agtacttccc tcagtcgttc atctgcggtt cccacccgga cacatttcat
    23581 ttttccgggc cacatcggat atttcccggt gcctgccgca aacgacatat acataagtgc
    23641 cagcgttgcc cagaatgcgg tgaccagttg catcagtgac cggtcagtct ccctggcata
    23701 ctctgtggct ccagcattgc ggtaatggca gatttctttt tcttctgctc cgggaaggtc
    23761 tgaggaagaa aaccggcatt cactgaccgg aaactgcccg gcagtggctg tctgtcttac
    23821 aaagttaatc tcctgaacca gtaatttccc cggcgtggtg ttgtccttcc ataaaacaaa
    23881 taacgccatc agggccattg tggccagtgg cagaatgacg gtgtatttca gacaccagaa
    23941 cagtccacgc agaacccggg cgacaacata ccgggcggca tggcacgcct gacgggacga
    24001 cgtattattt gccatcgttg cccccttcct gaccaccgga cataaactgc tgcggattca
    24061 ggtcgcccag ctgcttcagc atctggtcgg tattcagacc attcaggtga ctctctgcgg
    24121 atggcggatt cggcacggga cttcccgggt tactctcttc cgcctgcccc tgtgcctgct
    24181 ccagcgccat ctcttccacg gttttcagct ggaaaccgtc ctggaacacc acggtcactt
    24241 cattacccgc cccgatgggt atcaccgggt gatactgctc agcgcgtttg atgtagtagt
    24301 cactgagtgt ctgtgccgct ttcgatgcac cgccaccgat ccccattttc aggacatcgc
    24361 cggccccgta agcggcagtg gctcccagcc cgacagctgg ctgtgaagct cgctccattc
    24421 cctgaccgat accgtcaaca aatcccgcgc cccatgccca gccgaggatt ttgccgttac
    24481 gcatcaccac ttcacccttg ataccgtttt taccccggaa gctgacatgc cctttaaccg
    24541 gcatatcgat ggttttaccc tccttcaggc aactgatatt gcgggtacgc acgatggcac
    24601 gctcactgga cacatccccc caggcttcca gcccaacaaa gcatcccgtt gcgtcatagg
    24661 ttttgctgtt cggcatttcc accagaccgg tgatacgcag ctgcatcggc accgtggatt
    24721 cattaccggt gacagaggca ttggcatccg ccccttcaat cagaatggct ttcgcaaaac
    24781 tgcctgacgg aatgtacggc agggacggac cttgctttgt gccagcatca tagctgaacg
    24841 ttttacgctg tatccggttc ggcaccggca cagactggta cgtcacctgt ggcggcggcg
    24901 tgactccgtt gcccggatag aacgcggtgg gcggtggcac tgcacctgcc ccctgcggcg
    24961 gaaaagcaac gggcgcatta ccgggctgag gctccccctc cggaccggga ggggatgccg
    25021 gcatctgtgg aactggctct ccggtcactg taacgggatt cgcgcccagg gctttcacct
    25081 gctctgccag cgcagcattc tcctgcccca gcttttcaat gcgacgctgg tcatcaccac
    25141 gctgtttgtt cagaacatcc atctcacgac ggatttcctc atactgcttc tgcatctgtg
    25201 ccgccgtcac ctgcatctct gtggtggcat gctgacgcac cttgtcatca aaggtggtat
    25261 ccacaacacc ggtcatatcc ggcacaggct cctgccctgc aacggcatca gtgttcccgg
    25321 acatatccat atcagacaga taaagcgcac caccaattgc tgataccgat ccgacaatca
    25381 caatccccag ccacaggaac tgcttgcgtt tcacaatggt gttgatactg gccatcagtt
    25441 gccctccccg tcacgaatga cgtacacatc catacgggca gcggccagta actggctgac
    25501 aggctggctg aacatcaccg cccgggttcc cggctgccag aagtcacttt cccggatatt
    25561 cagcgcagac agcgttttat tttccacggt aaagcgcacc atcttcaggt gattccccgt
    25621 ccatacggct tcagcttttg cggacattcc ggcaggggcc tgcagggttt cctttgtcac
    25681 ggggatctga taccagcctt ctggcatttt tccggcacgg accgcctgac tgatggaaac
    25741 cagcagggat tcgtaaggcg tggatgtttc ccatgcaccg gcagtttctc ctgttccgcg
    25801 caaatcgctg accagctgaa tggtacgccc ggctccttca cggggaacgg cctgaatgga
    25861 aagattaaga ccacgttctg tttccagaat gaacgtaaaa ggctttttgt tgacggtggc
    25921 caccaccaca ccaccggatg cagtctgctc attattggtc agcgcaccat ccagactgtt
    25981 cacggcgata atccggtctc cggggacggt aaacagattc gggctggtat tactgatagc
    26041 aatacttgcc tgaccgccat tgaccatcgg aaccactgta ggtgccagtg tgccgtttcc
    26101 tgccagcacg ctgccggaaa aaagcaggca ggcggatatt attactgtct tattttttct
    26161 catcgtctgt ttccccgaaa ttatccagcc aggtcacccc gttttcccgt ttcagaataa
    26221 gaatgtaatg tttgatatca gtgaaaggtt cggagttacc aatccatgtt ttccgaatcc
    26281 cacggatttc cacacgacca tattgtggcc agacacgaac actggtctgg aaaaaagcag
    26341 aattcacgtt atcttttttg atgagctttg cttcttcggc cagaatcact ttcatcttat
    26401 tctgcgcgcc aggacggata tactgcagaa gtgcctggtg agaagcatcg acgatttccg
    26461 gtgagacatt caggcgcagg gcaataaacg ccagcgccat ctgctgcaaa aaagaggcat
    26521 cagcactgtt ctgtgatacc gcaaagggca tattaaatcc catcggtgtc acggctgtac
    26581 gctgttcatt ctgaaggcgg taattattca ccccctgaat cacgttaaca gtcagactga
    26641 gaacaatgag cagggacata aatataaaag caatggccag tacacgactg gtacttaagc
    26701 gggcaccgtg ttccatataa caatccaggt atcagttcta tttaatccac tgccggaaac
    26761 acgaatcagg aacattatga aaaataccgc gcagcaggac tgttggcata taccagtaaa
    26821 taagatcccg taaccaggag ctgccccgcc cttttttcag tttttttatc ccgaaataaa
    26881 ccagaaccgc cgcaccaata ccgaacagat atttcgatgt ttcgataccc cagccaatac
    26941 agatcgctgc ggggatcagt tcatccagtg gcaggccaaa ccagcggctc tggttggtca
    27001 gtgtttccgg gaaacgatat ttcttaattt tattctcgtc tcccgacatc gttttatttc
    27061 ctgtcagagg ccaacgacgg tcatacccac agcaataaat acagatatga tggcaaaacc
    27121 ggccaggaac ttgacgtttt tggtcatcat gtacatgacc gccccgacca gcacttcagc
    27181 cagaacgacc catttaacaa tactggagtc cttaccaaag gtggctttga cggtgtcatt
    27241 cccggactgc atgaggtcct gaccgccagc agccagggcc atctgaggga agaacatcat
    27301 cagcacagca gcggggatca cagcacgaac caggcgaagc atattcagac gcgtgaattt
    27361 agaaaaaaac gatttctttt cgacgggcgc agaagcaccc tgaacactca aaatagcatt
    27421 cataagttac ctctattgtg gatagattaa taaacttaaa gatctttaat tatcgaatca
    27481 gtttcccctg ttccctcctt tattgcatcc agattataaa agtcaggaaa acggctcaga
    27541 tgatcacgta atctgactag cacctcatta gtttttgttc tcccgctacg ctcccgggca
    27601 cctaagagct tgtgattagt agcatcgtcc aataccagta aaactttatt gcccggagca
    27661 ggccttatta tatttctgct caagtc
//
LOCUS       DAZGAP010000053        27583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGAP010000053 DAZGAP010000000
VERSION     DAZGAP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.053"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..570
                     /gene="cmtB"
                     /locus_tag="ACHLAM_001890"
     CDS             127..570
                     /gene="cmtB"
                     /locus_tag="ACHLAM_001890"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO7853422.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILNQLLTAASEKQLADIISRG"
     gene            598..1986
                     /gene="cmtA"
                     /locus_tag="ACHLAM_001891"
     CDS             598..1986
                     /gene="cmtA"
                     /locus_tag="ACHLAM_001891"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO7853423.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            2001..3278
                     /gene="yggP"
                     /locus_tag="ACHLAM_001892"
     CDS             2001..3278
                     /gene="yggP"
                     /locus_tag="ACHLAM_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7853424.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3275..3590
                     /locus_tag="ACHLAM_001893"
                     /pseudo
     CDS             3275..3590
                     /locus_tag="ACHLAM_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3612..4121
                     /gene="fumE"
                     /locus_tag="ACHLAM_001894"
     CDS             3612..4121
                     /gene="fumE"
                     /locus_tag="ACHLAM_001894"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO7853425.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            4118..4831
                     /gene="yggC"
                     /locus_tag="ACHLAM_001895"
     CDS             4118..4831
                     /gene="yggC"
                     /locus_tag="ACHLAM_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO7853426.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            5116..6135
                     /gene="epd"
                     /locus_tag="ACHLAM_001896"
     CDS             5116..6135
                     /gene="epd"
                     /locus_tag="ACHLAM_001896"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO7853427.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6185..7348
                     /gene="pgk"
                     /locus_tag="ACHLAM_001897"
     CDS             6185..7348
                     /gene="pgk"
                     /locus_tag="ACHLAM_001897"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO7853428.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            7562..8641
                     /gene="fbaA"
                     /locus_tag="ACHLAM_001898"
     CDS             7562..8641
                     /gene="fbaA"
                     /locus_tag="ACHLAM_001898"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO7853429.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            8832..9692
                     /gene="mscS"
                     /locus_tag="ACHLAM_001899"
     CDS             8832..9692
                     /gene="mscS"
                     /locus_tag="ACHLAM_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO7853430.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            9831..10466
                     /gene="argO"
                     /locus_tag="ACHLAM_001900"
     CDS             9831..10466
                     /gene="argO"
                     /locus_tag="ACHLAM_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO7853431.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            10559..11299
                     /gene="yggE"
                     /locus_tag="ACHLAM_001901"
     CDS             10559..11299
                     /gene="yggE"
                     /locus_tag="ACHLAM_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO7853432.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            11467..12363
                     /gene="ygfI"
                     /locus_tag="ACHLAM_001902"
     CDS             11467..12363
                     /gene="ygfI"
                     /locus_tag="ACHLAM_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7853433.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(12360..13838)
                     /gene="scpC"
                     /locus_tag="ACHLAM_001903"
     CDS             complement(12360..13838)
                     /gene="scpC"
                     /locus_tag="ACHLAM_001903"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO7853434.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(13862..14647)
                     /gene="scpB"
                     /locus_tag="ACHLAM_001904"
     CDS             complement(13862..14647)
                     /gene="scpB"
                     /locus_tag="ACHLAM_001904"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO7853435.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(14658..15653)
                     /gene="meaB"
                     /locus_tag="ACHLAM_001905"
     CDS             complement(14658..15653)
                     /gene="meaB"
                     /locus_tag="ACHLAM_001905"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO7853436.1"
                     /translation="MINEAKLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(15646..17790)
                     /gene="scpA"
                     /locus_tag="ACHLAM_001906"
     CDS             complement(15646..17790)
                     /gene="scpA"
                     /locus_tag="ACHLAM_001906"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO7853437.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(17994..18887)
                     /gene="argP"
                     /locus_tag="ACHLAM_001907"
     CDS             complement(17994..18887)
                     /gene="argP"
                     /locus_tag="ACHLAM_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO7853438.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            19028..19213
                     /locus_tag="ACHLAM_001908"
     CDS             19028..19213
                     /locus_tag="ACHLAM_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853439.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            19269..19928
                     /gene="rpiA"
                     /locus_tag="ACHLAM_001909"
     CDS             19269..19928
                     /gene="rpiA"
                     /locus_tag="ACHLAM_001909"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO7853440.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20184..21416
                     /gene="serA"
                     /locus_tag="ACHLAM_001910"
     CDS             20184..21416
                     /gene="serA"
                     /locus_tag="ACHLAM_001910"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO7853441.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLAEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21645..21704
                     /gene="ibsC"
                     /locus_tag="ACHLAM_001911"
     CDS             21645..21704
                     /gene="ibsC"
                     /locus_tag="ACHLAM_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO7853442.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(21805..22407)
                     /gene="fau"
                     /locus_tag="ACHLAM_001912"
     CDS             complement(21805..22407)
                     /gene="fau"
                     /locus_tag="ACHLAM_001912"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO7853443.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(22428..22611)
                     /gene="ssrS"
                     /locus_tag="ACHLAM_001913"
     ncRNA           complement(22428..22611)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHLAM_001913"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22653..22982)
                     /gene="zapA"
                     /locus_tag="ACHLAM_001914"
     CDS             complement(22653..22982)
                     /gene="zapA"
                     /locus_tag="ACHLAM_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7853444.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALFEQGRITE
                     KTNQNFE"
     gene            23150..23728
                     /gene="ygfB"
                     /locus_tag="ACHLAM_001915"
     CDS             23150..23728
                     /gene="ygfB"
                     /locus_tag="ACHLAM_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO7853445.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23754..25079
                     /gene="pepP"
                     /locus_tag="ACHLAM_001916"
     CDS             23754..25079
                     /gene="pepP"
                     /locus_tag="ACHLAM_001916"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO7853446.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            25076..26254
                     /gene="ubiH"
                     /locus_tag="ACHLAM_001917"
                     /gene_synonym="visB"
     CDS             25076..26254
                     /gene="ubiH"
                     /locus_tag="ACHLAM_001917"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO7853447.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            26277..27479
                     /gene="ubiI"
                     /locus_tag="ACHLAM_001918"
                     /gene_synonym="visC"
     CDS             26277..27479
                     /gene="ubiI"
                     /locus_tag="ACHLAM_001918"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO7853448.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         6584 a         6765 c         7368 g         6866 t
ORIGIN      
        1 tttaaagttt tattattttt gttcatgggg tttttcttct cgcgtaccca attgctttca
       61 ttaaaccgca cgagtatttt atttttaccg ccggacggtg ccctaaaaat aaaataagga
      121 ttatttatgc ggcttattga ttattttcca gaatcatcaa tctcagttat acattcagca
      181 aaagattggc aggaagctat cgatttctcg atggcatcat tgctggataa aaactatatc
      241 agcgagaatt acattcaggc aattaaagat tccaccatta acaatggccc ttattatatt
      301 ctcgcaccag gcgtggcaat gcctcatgct cgaccggaat gtggggcgct taaaaccggg
      361 atgtctttaa cattacttaa acaaggcgtt tattttccgg ggaatgacga accgataaaa
      421 ttactcatcg ggctttctgc tgccgatgcc gattcgcaca ttggcgctat tcaggcgtta
      481 agtgaattat tgtgtgaaga agaaatactc aaccaattat taacagcagc atcagaaaaa
      541 caattagcgg acataatcag ccgcggataa ttactctctc ctctacagga taatattatg
      601 gaaaacaagt ctgctcgtgc aaaggtccag gcttttgggg gctttttgac tgcaatggtc
      661 atccccaata tcggcgcttt tatagcctgg ggttttataa cggcgttatt tattcccacc
      721 ggctggctgc ctaacgaaca tttcgccaaa attgtcggcc cgatgattac ctatttattg
      781 cccgtgatga ttggttctac cggtggtcat ctggtcggcg gtaaacgcgg cgcggtcatg
      841 ggcggaatag gtactattgg tgtgatcgtc ggcgcagaga tcccgatgtt ccttggctca
      901 atgattatgg ggccgctcgg tggtctggtg attaaatata tcgacaaggc gctggaaaag
      961 cggatccctg ctggttttga gatggttatc aataacttct cattaggtat tgcggggatg
     1021 ctcctttgtc tgctgggttt tgaagttatc ggcccggcgg tgttaattgc caatactttc
     1081 gtcaaagagt gtattgaggc gttggtacat gcgggttatc tgcctttgct gtcagtcatc
     1141 aatgaaccgg cgaaagtgct tttcctcaat aacgcgatag atcagggcgt ctattacccg
     1201 ttggggatgc aacaggcttc agttaacggt aaatccatct tctttatggt ggcgtccaac
     1261 cctgggcctg gcttaggact gttgctggcg tttactttgt ttggtaaagg gatgagtaaa
     1321 cgttctgcgc ccggggcgat gattattcac ttcctcggtg ggatccacga actgtatttc
     1381 ccgtatgtgc tgatgaagcc gctgaccatt attgccatga ttgcgggcgg tatgtctggc
     1441 acctggatgt ttaacttact ggacggtggt ctggtggctg gcccaagtcc ggggtctatc
     1501 tttgcttacc tggcactgac gccgaaaggc tcgttcctgg cgacaattgc cggtgttacg
     1561 gtaggtaccc tggtgtcctt tgctatcact tccgtaatac tgaagatgga aaaaacggtg
     1621 gaaacggaga gcgaagatga gtttgctcag tcagccaatg cggttaaggc gatgaaacaa
     1681 gaaggtgcat tctcgttaag cagggttaag cgtatcgcct ttgtttgcga tgcggggatg
     1741 ggctccagcg cgatgggcgc gaccaccttc cgtaaacgcc tggaaaaggc ggggctggca
     1801 atagaagtaa aacattacgc gatagaaaac gtgcctgcgg atgcggatat cgttgttact
     1861 catgccagtc tggaagggcg cgtgaaacgt gtgacggata aaccgctgat actgattaat
     1921 aactatattg gcgatccaaa actcgacact ttatttaatc aattaaccgc cgaacataaa
     1981 aactgattgc cgaggtaaaa atgaaaacca aagttgctgc tatttatggc aagcgggatg
     2041 tccgtctgcg cgaatttgaa ctgccagaaa ttaccgataa tgaactgctg gtgagtgtga
     2101 tttctgacag cgtgtgttta tccacctgga aagcggcgct actcggtagt gaacataaac
     2161 gcgtacccga cgatttagaa aatcatccgg tcattaccgg gcatgaatgc gccggggtta
     2221 ttgtcgaagt gggtaaaaat ctcaccgaca aatataaaaa aggccagcgt tttgtattgc
     2281 aaccggcaat ggggttacca agcggatatt cagcaggcta cagctacgaa tattttggcg
     2341 gtaacgccac ctatatgatt attcctgaaa tagccattaa cctgggctgc gtattaccgt
     2401 atcacggctc ttattttgct gcggcgtcgc tggcagagcc tatgtgctgc attattggcg
     2461 cttatcatgc caattatcac accacgcaat atgtttatga gcatcgcatg ggcgttaaac
     2521 ctggcggcaa tattgcgctg ctggcatgtg ctggtccgat gggcattggc gctatcgatt
     2581 atgctattaa cggcggcata caaccgtcgc gggtggtggt ggtcgatatc gacgacaaac
     2641 gcctggcgca ggtacaaaag ctgctgccgg tggaactggc ggccagtaaa ggcattgagc
     2701 tggtgtatat gaataccaaa gggatgagcg atccggtcca gacgctgcgg gcgctgacgg
     2761 atgatgccgg atttgatgac gtatttgtct atgcagcggt gccttcagtc gtcgagatgg
     2821 ctgatgaatt actggcggaa gatggctgcc tgaacttctt tgccgggccg acggataaaa
     2881 acttcaaagt gccgtttaat ttctacaacg tccattacaa cagcacgcat gtcgtcggta
     2941 cgtccggcgg ttcaacggac gacatgaaag aggcgattgc gcttagcgcc acagggcagt
     3001 tgcagccgtc gtttatggtg acccatatcg gtgggctgga tgcggtgcct gataccgtgc
     3061 tcaatctgcc ggatatcccc ggcggtaaaa aactcattta taacggcgtc accatgccgc
     3121 tcactgccat tgccgatttt gccgaaaaag gcaaaaccga tccgctgttt aaagagctgg
     3181 cgcggctggt tgaggaaacg cacggcatct ggaatgagca ggccgagaaa tatctgctgg
     3241 cgcaatttgg cgttgatatc ggggaggccg cgcaatgatg tccctggcgt ggccattatt
     3301 tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa accggatgtg gcgacaaaaa
     3361 taaaattgat ggcctggcgg tcaccgcgat gcgccaggca ttaaacgacg tcgcttttcg
     3421 tgggcgagtg gttatcggcg agggggaacg atatcctctt tagcgctacg ggcgtgacgg
     3481 gcggcgagtt ggtgaacggt atccaacaga cggcgaatgg ggtgcggacg cagacattac
     3541 tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga tggtgatcta
     3601 tcggaagacc aatggcgacg ctgacagaag atgatgtgct tgagcaactg gatgcacagg
     3661 acaatttatt ttcatttatg aaaactgcgc attctatttt gctccagggg atacgccagt
     3721 ttctgccgtc gctgtttgtc gataacgatg aagagatcgt cgaatatgca gtgaagccgt
     3781 tactcgccca aagcggcccg cttgacgata ttgatgttgc gctgcgtttg atttatgcgc
     3841 tggggaaaat ggataaatgg ctgtacgccg atatcacgca tttttcccag ttttggcatt
     3901 acctgaacga acaggatgaa acgcccggat ttgccgatga catgacctgg gattttatca
     3961 gcaacgtcaa tagcataacc tgcaatgcga cgctctatga cgcgttgaaa gcgatgaagt
     4021 tcgccgattt cgccgtctgg tcagaggcgc gttttagcag gatggtcaaa acggcgctga
     4081 cgctggcagt aacgaccacc ttaaaggaat taacgccgtg aaaattgaat taacggtgaa
     4141 tgggctgaag atacaggcac agtaccagaa cgaagagatt gaaaatgtac ataaaccgtt
     4201 gctgcacatg ttggcggcct tacagacggt aaatccgcag cggcgtacgg tggtttttct
     4261 ttgcgctccg ccggggacag gtaaatctac cctaaccacc ttctgggaat atctcgcaca
     4321 acaagacccg gaactgcccg ctattcagac actgccgatg gatggttttc accattacaa
     4381 tagctggctg gatgcgcatc aattgcgccc cttcaaaggc gcaccagaga cattcaacgt
     4441 tgcgaaactg gcggaaaatc tgtgccgggt tgtggaaggg gattgtacgt ggccgcagta
     4501 cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac cactcgtcat
     4561 cgtcgaagga aactggctat tactggatga tgaaaagtgg caggagttgg catctttctg
     4621 cgatttttct atttttattc atgcacctgc tcaaattctg cgggagcgct tgattagtcg
     4681 taaaattgcg ggaggattat cgcggcaaga agccgaagct ttttatgccc gaaccgatgg
     4741 tcccaatgtc gagcgtgtgc tggtgaatag tcgtcaggcc aatctgatat tagagatgac
     4801 cgaagaaggc agatatcact tcaaaagctg acaatttatt ccagttattg caggggtatt
     4861 attacgccag cctggaataa agccccctac aaaaggggat aaaagtgtga tgtaagtcag
     4921 ataaatgtct tcttcggctg gacaatcatt ccttttattc cacgtttcgc ttatcctagc
     4981 tgaagcgttt cagtcgatta aatgttcgac aattaaccaa tcagtcgcag tttgcgacag
     5041 gtaaggtttc cccggacgat ttgctggatt actctgtctg gcaaatttgc tcagggaaaa
     5101 ccttgcagga gatctatgac cgtacgcgta gcgataaatg gcttcggtcg catcgggcgt
     5161 aatgtggttc gtgctttgta tgaatccgga cgccgggcgg aaattaccgt ggtggcaatc
     5221 aacgaactgg cggatgctgc gggcatggcg catttgttga aatatgacac cagccatggc
     5281 cgttttgcat gggaagtacg acaggaacgc gatcaacttt ttgttggtga tgacgccatc
     5341 cgtgtattgc atgaacgttc actgcaatcg ctcccctggc gtgaacttgg cgttgatgta
     5401 gtcctcgact gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca tattgccgcc
     5461 ggggccaaaa aagtgctctt ttcacatcca ggcagtaacg atctcgacgc gaccgttgtt
     5521 tacggcgtca atcaggatca acttcgtgcg gaacaccgca tcgtttctaa cgcttcctgt
     5581 accacgaatt gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg tattgagtcc
     5641 ggcactgtga ccacaattca ctccgccatg cacgatcaac aggttattga tgcataccat
     5701 cctgacctgc gtcgcacccg ggctgccagc cagtcgatca ttccggtcga tactaaactg
     5761 gccgccggta tcacacgatt ttttccgcaa tttaacgatc gctttgaagc gattgcggta
     5821 cgtgtgccaa ccataaatgt gacggcaatc gatttaagcg tgacggtgaa gaaacctgta
     5881 aaagccaatg aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt tcatggtata
     5941 gttgactata cggaattgcc gttggtctct gtagatttta accacgatcc gcacagtgcc
     6001 attgtcgatg gcacccaaac ccgggttagt ggcgcacacc tgatcaaaac gttggtctgg
     6061 tgcgataacg aatggggctt tgctaaccga atgctcgaca cgacgttagc tatggctact
     6121 gttgctttca ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa cgagaggatt
     6181 caccatgtct gtaattaaga tgaccgatct ggatcttgct gggaaacgtg tatttatccg
     6241 tgcggatctg aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc gtatccgtgc
     6301 ttctctgccg accatcgaac ttgccctgaa acaaggcgca aaagtgatgg taacttccca
     6361 cctgggtcgt cctaccgaag gcgagtacaa cgaagaattc tctctgctgc cggttgttaa
     6421 ctacctgaaa gacaaactgt ctaacccggt tcgtctggtt aaagattacc tcgacggcgt
     6481 tgacgttgct gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca aaggcgagaa
     6541 gaaagacgac gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt tcgtaatgga
     6601 cgcattcggt actgctcacc gcgcgcaggc ttctactcac ggtatcggta aattcgctga
     6661 cgttgcgtgc gcaggcccgc tgctggcagc tgaactggac gcgctgggta aagcactgaa
     6721 agaacctgct cgcccgatgg tggctatcgt tggtggttct aaagtatcta ccaaactgac
     6781 cgttctggac tccctgtcta aaatcgctga ccagctgatt gttggtggtg gtatcgctaa
     6841 cacctttatc gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag ctgatctggt
     6901 tgacgaagca aaacgtctgc tgaccacctg caacatcccg gttccgtctg atgttcgtgt
     6961 agcaactgag ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg atgtgaaagc
     7021 tgacgagcag atcctggata tcggtgatgc ttccgctcag gaactggctg aaatcctgaa
     7081 gaatgcgaaa accattttgt ggaacggtcc ggttggcgtg ttcgaattcc cgaacttccg
     7141 caaaggtact gaaatcgtgg ctaatgctat cgcagacagc gaagcgttct ccatcgctgg
     7201 cggcggcgac actctggcag caatcgacct gttcggcatt gctgacaaaa tctcctacat
     7261 ctccactggc ggcggtgcat tcctcgaatt cgtggaaggt aaagtactgc ctgcagtagc
     7321 gatgctcgaa gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc tgcccttgtt
     7381 gcagcgcgct tttaaagcac gcactttttc atactctaaa taattcgagt tgcaggaagg
     7441 cgacaagaga gtgaatccca tgagcttaca taagtaagtg actggggtga gcgaacgcag
     7501 acgcagcaca tgcaacttga agtatgacga gtataaggcc cgacgataca ggacaagaga
     7561 catgtctaag atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa
     7621 agttttccag gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac
     7681 tgactccatc aacgccgtgc tggaaaccgc tgctaaagtt aaagcgccgg ttatcgttca
     7741 gttctccaac ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca
     7801 gggtgctgct atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca
     7861 ttatggtgtt ccggttatcc tgcacaccga ccactgcgcg aagaaactgc tgccgtggat
     7921 cgacggtctg ctggacgcgg gtgaaaaaca cttcgcagct accggcaagc cgctgttctc
     7981 ttctcacatg atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa
     8041 atacctggag cgcatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg
     8101 tggtgaagaa gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc
     8161 ggaagacgtt gattatgcgt ataccgagct gagcaaaatc agcccgcgtt tcaccatcgc
     8221 agcgtccttc ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac
     8281 catcctgcgc gactctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct
     8341 gaacttcgta ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag
     8401 ctacggcgta gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt
     8461 tctgaactac tacaaagcga acgaagctta tttgcagggt cagctgggta acccgaaagg
     8521 cgaagatcag ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac
     8581 ttcgatgatc gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta
     8641 agtcattcct ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca
     8701 aggtgatgca gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc
     8761 ttgtcagact gcccgccata aggcggcgga gtgtatttct ccattttgag tcagttgaaa
     8821 aggaatattg aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc
     8881 tggtggctaa ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga
     8941 tcatcattgt tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga
     9001 tctcccgtaa aatcgatgcc actgttgctg attttctctc tgcattagtc cgttacggta
     9061 ttatcgcctt tacgctaatc gctgcactgg ggcgcgtggg cgtacaaaca gcgtcagtca
     9121 ttgctgtact cggtgccgca ggcttagcgg ttggtctggc tttgcagggg tcactgtcta
     9181 acctggccgc tggcgtgtta cttgttatgt tccgcccgtt ccgtgccgga gaatatgttg
     9241 atctcggcgg cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta
     9301 ctgcagacgg taaaattatc gttattccga acggtaaaat tattgccgga aatattatta
     9361 acttctctcg cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg
     9421 atatcgatca ggttaagcag atcctgaccg atattatcca gtctgaagat cgcattttga
     9481 aagatcgcga aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg
     9541 tccgcgtctg gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta
     9601 ttaaacgtga atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact
     9661 ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct
     9721 ggcctctctc ttattagttt ttctgattgc caattaatat tatcaatttc cgctaataac
     9781 aatcccgcga tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt
     9841 attactttca aggtcttgca cttggggcgg ctatgatcct accgctcggt ccacaaaatg
     9901 cttttgtgat gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg
     9961 ctatcagcga tttggtcctg atttgcgccg ggatttttgg tggcagcgcg ttattgatgc
    10021 agtcgccgtg gttgctggcg ctggtcacct ggggcggcgt agccttcttg ctgtggtatg
    10081 gttttggcgc ttttaaaaca gcgatgagca gtaatattga gttagccagt gccgaagtcc
    10141 tgaagcaagg cagatggaaa attatcgcca ccatgctggc agtgacctgg ctgaatccgc
    10201 atgtttacct ggatactttt gttgtactgg gcagtcttgg cgggcaactt gatgtggaac
    10261 caaaacgttg gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg
    10321 ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca
    10381 atctggtagt gggatgtgtt atgtggttta ttgccttgca gctggcgaga gacggtattg
    10441 ttcatgcaca agccttgctc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct
    10501 aagcttgcct ccagaggtcc tgaattttag gcaagtcaat taacacggag agactaacgt
    10561 gaagttcaaa gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc
    10621 taacgaattg ccggatggac cgcatattgt cacctccggt acggcaagcg tggatgcggt
    10681 gccagacatt gccactcttg cgattgaagt caacgtggcc gcgaaggatg ccgctactgc
    10741 caagaaacag gcggatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat
    10801 cgcgaaaaaa gatatcagct cagcgaactt acgcacccag ccagattatg attatcagga
    10861 tggtaaaagt atcctgaaag gctaccgtgc tgtgagaacg gtggaagtca cgctccgtca
    10921 gttagacaaa ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg
    10981 ttctgtatcg ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc
    11041 gattgataac gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg
    11101 gccggtatat agtgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat
    11161 gatgaaagcc gatgccgcgc cggtgtccgc ccaggaaact tacgagcagg ccgctattca
    11221 gtttgatgat caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa
    11281 aacacctgca gcacaataat atttgtagcc cgacgtattc gtcgggctaa tgcctttacc
    11341 cgatattgcc tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg
    11401 ctcgcggttt catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggga
    11461 gggggtatgg acatttttat ttcgaaaaag atgcgcaatt tcattttatt agcgcaaacc
    11521 aataatattg cccgggcggc agaaaaaatc catatgacag cttcgccatt tggtaaaagc
    11581 attgccgcgc tggaagaaca aattggctat acgctattta cccgcaaaga taataatatc
    11641 agcctcaata aagccggaca ggaactgtat caaaaactgt tcccggttta tcaacggctt
    11701 tctgcgattg ataatgaaat ccataactca gggcgtcgtt cacgagaaat tgtgattggc
    11761 atagataata cttatccgac aattattttc gatcagttga ttagtctcgg agataagtat
    11821 gaaggcgtga ccgcccagcc agttgagttc agcgaaaatg gcgttatcga taatcttttt
    11881 gaccgtcagt tagattttat tatctcccca cagcatgtat ctgcacgagt tcaggaactg
    11941 gaaaacctga ctatcagtga actaccgccg ctgcgattgg gctttctcgt ttctcgtcgt
    12001 tatgaagagc gacaagagca agagttgctt caggagctac cctggctaca aatgcgcttt
    12061 cagaaccggg ctaacttcga agcaatgatt gatgcgaata tgcgtccttg tgggatcaat
    12121 cccaccatta tttatcgtcc ctacagtttt atggccaaaa tcagcgccgt ggagcgcgga
    12181 cattttctga cggtgatccc gcattttgcc tggcgactgg tgaatccggc aacgctgaaa
    12241 tattttgatg cgccgcacag gccgatgtat atgcaggaat atctttattc aatcagaaat
    12301 catcgttata ccgccacgat gcttcagcat attgctgaag atcgtgacgg gacgagtcat
    12361 taacccagca tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga
    12421 tcgtggtgaa tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac
    12481 ataggatgtg cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccacgc
    12541 agatcggcga tgccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca
    12601 tggctgcaca ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggggccata
    12661 aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc caccgatgcc gttcatcaga
    12721 tcgaccccgg ctacgtgcgt tgagttggca tgcccgtaaa tatcaaactc caggccgacg
    12781 ttcagagcga tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga
    12841 cgcaacacaa tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg
    12901 gccgagatag tcaggctgga ggcgctggcc ccgctgattt tgcccgtttc cagtaaatgc
    12961 accaccgatt cctgtagcac ttccgaatac atcataaacg gaggaatttc cggattttcc
    13021 cccagacgcg ccattaccgc attattgatg ttgcctacgc cactttgcag cggcagaaat
    13081 tccggcggaa tacgcccatg cgccatttcc tgcaacaaga acgtgaccac gttatcggca
    13141 atctgctggc acatgggatt ttgcttatcc agcatattac cggcgtcagg caagttggtt
    13201 tccacgacgg cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc
    13261 attgcatgga agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct
    13321 gccagttctg caacgcgcgg atcgtgatag tgattgagtt caatgatcac tttcttcgcc
    13381 cgcagcagcc aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt
    13441 gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc
    13501 atttgcgcca cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt
    13561 ttacgtaaac cggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa
    13621 agtacatcgt cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc
    13681 tttttggcct catgctgttc gttagctctg cgggcaatcg cgttgggtaa ggctttcggc
    13741 gaacccgctg gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc
    13801 gcttcatcgg cggtcatcct tgtccactga gtttccattt taactccttc gttcgcaggg
    13861 attaatgacc aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt
    13921 aatcttcgct gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt
    13981 tcatggtgtg cgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg
    14041 gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt
    14101 ccacaacatg gttgaggatg ccgacagcca gcgcgcgctg ggctgtgatt ggcgaagcgg
    14161 taaaaatcag ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga
    14221 ccaggttata cgggacgccg aggtttacag gtgtcattga gaaggttgag gtactggcgg
    14281 cgatgatcag atcggaactc atgatcattt caaatgcgcc accccagaca ctaccttcca
    14341 ccatcgaaat gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaacggat
    14401 catcatagga gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg
    14461 agaagacttt ggaaccactc ggcgcgcgca aaatgataca gcgaatttcc ggccggttga
    14521 gatcgcttaa tgcctgcata agatcatcaa taaagacttt acttaaggca ttaagtttgc
    14581 ggccatagtt aaactcaatg accgccactt tgttgatagt gacaacgttc acatactgat
    14641 aagacataaa aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc
    14701 aggccggtgc gcggtgagag cgtattgttt ttgactgcta aaagcgtctg gcggtaatag
    14761 cgatcgaaat cttcattcgc gaacaggcga ttcagtactt cttcttcggt ctgcttacgc
    14821 agccattcca ccgattgttg ttgccgcact tgttgtaatc gaccactggc agttagcgcg
    14881 gttttgaagt cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta
    14941 caagtcagaa cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc
    15001 tcgtacatat gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata
    15061 acgatcagat ctgccacttc catcagcccc tttttaatgc cctgcagatc atcgccgcca
    15121 ccggcaattt gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttctgactgc
    15181 ccgacgccaa ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt
    15241 aattcccgcg ctcgctgact ggcaccgccc agatggccgg aggagggtac cgggcgaata
    15301 aacgccgcct cggcacgcgc aaggtcattc atgcgggttt tatccccgag aatgctaccg
    15361 ccagtgaccg ggctgctggg atcgaccgca ataaccgcaa cctttaatcc ctcacgaatc
    15421 aacaacatgc caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg
    15481 cccagtcgca gggcgttacc acagaacggc ataatggcgt caagcagctg tgtacttagc
    15541 gcctgatgac gcgggtgacg gctttccacc aacgtcatgg cctgggcgag cgtggcacgc
    15601 tcaccctgac gtaagcggcg aatactttct gctagcttgg cttcattaat catgatgctg
    15661 gcttatcaga ttcagtacgt cgcgcacgct gtcgagcata ggtgtacctg gaccataaat
    15721 cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc
    15781 gaccacgcag atatctttgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag
    15841 cgttttatga ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc
    15901 caggcgggcg atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag
    15961 atcggaatag gcgctggcga tcactttcgc gccgcgatcg tgcccatcct ggcccatttt
    16021 ggcgatcaga atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac
    16081 aatggcatcg aactcggcgg ccgatttctc agactgatga tagctttgcg caatcacgcc
    16141 ggtaacacac tggcttggca ccagataacg gtcgaaagcg gcttccagcg catcggaaat
    16201 ttcacccagg gtggcgcgaa cgcgagcagc attaacagcg gcagccagca ggttttcgtt
    16261 atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt
    16321 ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc
    16381 aagtacatcg gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg
    16441 cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg
    16501 cagacctgct tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc
    16561 tctggcttgt ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg
    16621 atcgacggtg cggcagagtt ctgattcttc ctgaatgatg atctgggtat tgcgggcaat
    16681 gcgtgctgag aaatcggtag gcaaaccaag cgcttcatca aaggcgttgg tatgcagtga
    16741 ctgagtaccg cccagcgttg cagccagtgc ttcaatggtg gtgcggataa cgttgttata
    16801 cggatcctgt tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga
    16861 tttcggatcc tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg
    16921 caacatggcg acgttcataa acagatccat gccaatgccg aagaagaacg acagacgagg
    16981 agcgaagtca tcaattttca gcccggcgga gattgctgct ttgatgtact caatcccatc
    17041 ggcgagcgta aatgctacct gctgcacgca gttggcaccc gcttcaccca tgtggtaacc
    17101 gctgatactg atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtctgc
    17161 gataatacgc attgacggtt ttggcgggta aatataggtg ttgcggcaaa gatactcttt
    17221 gaggatatcg ttttgaatgg tgccggtcag tttatcgggt gtaacacctt gctcttctgc
    17281 ggcgacaata taaaacgcca gtactggtag tactgcgcca ttcatggtca tcgaaaccga
    17341 cattttatcc agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc
    17401 aacgcccgct ttgccgacgt cgcccgccac gcgtgggtta tcggagtcgt agcctcggtg
    17461 ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata
    17521 aaaagcgttg gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg
    17581 ggcggtatac atagtggcac gcgggccacg aacgtaggga ggcaaaccag gaagggtacc
    17641 tgtcacctcc agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc
    17701 cgcggtttgc tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc
    17761 aagctgttgc cactcctgca ccttagacat tatttgctcc gtaaaacgca atcaaaatga
    17821 gggtatttgc gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat
    17881 tcagacgtcg ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac
    17941 aaaaagaagt gaggataacg cctgatatgt gctatcaggc attatttgat ggattaatcc
    18001 tgacgaagga ctttgtgacc ataatcgagc aacgcatcag tgactttacg catcatgcgg
    18061 ctttcaggag caaagcggtg ccagtagagc atccgtcgtt gaaatagccc tggcgttaag
    18121 tcaatcagtt caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag
    18181 gtggtgccct gacgagcaag ttgcacgaac gcttccgaag aattaacgat atggcagggt
    18241 acgctgccag gaggcagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga
    18301 tggtcaaaag cgaccacagg tgctttcaat aatgccgaac gcgttacgcc gttagggaaa
    18361 tatttttcgg caaacggttt tgagctgacg aacagatagt cgagcgcacc gagcttatcg
    18421 acaaggcaac tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg
    18481 cgcagacgtt cctgagtgcg ggtttcatct tctacctgca agttgaggcg gataggcgaa
    18541 tcagctaaca caggagccag cgccggaagc aaccacgtcg ccagactgtc agcgttgacc
    18601 gccagtgaaa gcagcagcgg agttgaaccg gtttgttcat cgcccagcca ctcttcttcc
    18661 agtaactcca cctggcgtag cagtgccagc agtttttgcc cttgttctgt cgggcgcggt
    18721 ggtacggtac gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt
    18781 gagacggctg attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt
    18841 atcaccgcat ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc
    18901 tcctggtaaa tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga
    18961 agtcgttggc gggtaacatt tgtatcagtg gttaacgcgc agcttaaaag atcagggatt
    19021 tgcgctaatg catttcgctc agcgagttcg cgccctggtt gtgttgaatg gcgtggcgtt
    19081 attgcctctg ttcgcatcgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg
    19141 gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca cccggcttgc
    19201 caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg
    19261 aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg
    19321 ttcagcccgg caccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg
    19381 cgctcggcac gatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg
    19441 aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca
    19501 tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg
    19561 cggcgctgac ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgccg
    19621 acgcctccaa gcaggttgat attctgggca aattcccgct gccagtagaa gttatcccga
    19681 tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc
    19741 agggcgtggt gaccgataac ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg
    19801 acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt
    19861 ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg
    19921 tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg
    19981 acatgtgtca cgtttttacc tggctattgt cgattgctct aaataaaccc tctaaaccag
    20041 catattcatc caagaaatac ctttgcgtga tatttcctca atatcgtgac gcaaacgttc
    20101 atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca
    20161 tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt
    20221 ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gcgggttaca
    20281 ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg
    20341 atgcccactt catcggcctg cgatcccgta cccatctggc tgaagacgtg atcaacgccg
    20401 cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg
    20461 cggcggcaaa acgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg
    20521 cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta
    20581 aagcgcatcg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa
    20641 agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc
    20701 tgggaatgta tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc
    20761 aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag
    20821 agaatccgtc caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aaacccggct
    20881 cgctgctgat taatgcttcg cgcggtactg tggtggatat tccagcgctg tgtgacgcgc
    20941 tggcgagcaa acatctggca ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca
    21001 atagcgatcc atttacctct ccgctgtgtg aattcgacaa tgtccttctg acgccacaca
    21061 ttggtggttc gactcaggaa gcgcaggaga atatcggtct ggaagttgcg ggtaaattga
    21121 tcaagtattc tgataatggt tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc
    21181 cactgcacgg tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggt gtgctgactg
    21241 cgctcaacaa aatttttgcc gagcagggcg tcaacatcgc cgcgcaatat ctgcaaactt
    21301 ccgcccagat gggttatgta gttattgata ttgaagccga cgaagacgtt gccgaaaaag
    21361 cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaaatcc
    21421 ccttatctga aaatcaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac
    21481 accgttgccg cattgcgcat tctactttgc gagtctgctc cagaaaataa atcaaagtca
    21541 gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg cagggcagca tggggctgta
    21601 acggtaaagc cctcaccgaa gcgagggctt gaaggagaag ggttatgatg cgacttgtca
    21661 tcatactgat tgtactgtta ctcataagtt tcccagctta ttaacagtca gtctcagggg
    21721 aggagtcatc ctcccttacc cttactcact aaattaggtt aaagaatcaa cgatgtcaat
    21781 cagggcgatg caggtgtgtc gccctcacca ctcccagact ttcgacggtg taaccaccgc
    21841 aggaagaggg atatcccact cttcaacggg gagtttttcc accaactgac aatcatgcgc
    21901 ataacccacc ggttgcattt tatagtgctg ccagttttgt aaggtccgat cataaaaacc
    21961 accgcccatt cccaggcgct gaccgtactc atcaaaggcg accagcggtg tgatcaacac
    22021 gtctaatcgg gaaaggggta gcacgtcacg cacatccagt ttgggctcat ggatcttcaa
    22081 cctgttcatc accagttcgc tttgcggatg gtaattcagg aacagcaaat taccggcact
    22141 gaagggatgc aaaactggaa gatatacgcg cttaccggcg cgccagagtt gttctatgag
    22201 tggcagggtg tcgagttcgc catcaaaaga gaggaataca gcgaccgtat gtgccatcac
    22261 caccggggga taagtcatca tccgggtagc ggcttgttga cccatttcct gctgttgttc
    22321 cggcgttaac gcacgacgac gttgccgaat cattttgcgg atttcttgtc gggataatgt
    22381 cagtgggagt tctggtagtt gcgtcatggc tggtgccaga taagaaggga atctccgaga
    22441 tgccgccgca ggctgtaacc cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt
    22501 taaggcttct cggacggacc gagcatgctc accaaccgcg gagcgccaca ttcttgtggt
    22561 atgaaatatc ggctcagggg actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc
    22621 acggttactc taccacagta aaccgaaaag tgtcattcaa agttttggtt agttttttcg
    22681 gtgatgcgac cttgttcaaa taacgcttgt tctatggtct gctgcagcat ccgaatacgc
    22741 tgttccatac ttgccgcata gtcacgagtc ttcgcttttt cttgcgctaa ctcatagctg
    22801 atattcaatg cggcaatgaa gaccaactgt tcagtatttg tgactctagt gcgttctttc
    22861 agatcttgca accgttggtt cagatcgtcc gctgcctgat tcaacgcatc cctttggtca
    22921 ggcgggcagt tcacacgcag tgaacggcca aaaatttgga tatcgacggg ttgtgcagac
    22981 atgccacctt cctgctgatt gactgcgctg ccttcgtctt tagacccatg gtctgcgaag
    23041 gggcgacact atagctaccc tgatgagaag agacaagccc ttttctggtc caccagggcc
    23101 caaagtggta gcatatcatg aatattcctc cctttgacga cgaatgctta tgtctataca
    23161 gaacgaaatg cctggttaca acgaaatgaa ccagtatctg aaccaacaag ggacgggtct
    23221 gacccctgct gagatgcatg gtttaatcag cgggatgata tgtggcggta acgatgacag
    23281 ctcatggctg ccgctacttc acgacctgac gaacgaaggc atggctttcg gtcatgagct
    23341 ggcacaggca ctgcgtaaaa tgcactctgc caccagcgat gccctgcagg atgacggctt
    23401 cctttttcag ctttatctgc ctgatggcga tgatgtcagc gttttcgatc gggctgatgc
    23461 gctggcaggt tgggtcaatc acttcctgct tggtcttggc gttacgcaac cgaagctgga
    23521 caaagtgacc ggagaaaccg gtgaagccat cgacgatctg cgtaacatcg cgcagctggg
    23581 ctacgacgaa gacgaagatc aggaagagct tgaaatgtcg cttgaagaga tcatcgaata
    23641 cgttcgtgtt gccgcgctgt tatgccacga cacctttact catccgcaac cgaccgcgcc
    23701 agaagtacaa aaaccgactc tacactaaaa acaaaaaacg taaggagagt gttatgagtg
    23761 agatatcccg gcaagagttt cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg
    23821 gcagcgccgc gctgattttt gctgcaccag aagtaacacg tagcgccgac agcgaatacc
    23881 cctatcgtca gaacagtgac ttctggtact tcaccggctt taacgaaccg gaagcggtgc
    23941 tggtgctgat taaaagcgac gacactcata accacagcgt tttgtttaac cgcgttcgcg
    24001 acctgacggc agagatctgg tttggtcgtc gcttaggcca ggatgccgcg ccagagaaac
    24061 tgggcgttga ccgcgcactg gcattcagcg aaatcaatca gcaactttat caactgctta
    24121 atggtctgga tgtggtttat cacgctcagg gcgaatatac ctatgctgat gaaatcgtga
    24181 acagcgcgct ggaaaagctg cgtaaaggct cgcgacagaa tctcactgcc ccggctacga
    24241 tgatcgactg gcgtcctgtt gttcatgaaa tgcgcctgtt caaatcacca gaagagattg
    24301 ccgtacttcg ccgcgcgggt gaaatcaccg cactggcgca tacccgggcg atggaaaaat
    24361 gccgtccggg gatgttcgag taccatctgg aaggcgaaat tcaccacgag tttaaccgcc
    24421 acggtgcgcg ttacccttcc tataacacca ttgttggcag cggtgaaaac ggctgcattt
    24481 tgcactacac cgaaaacgag tgtgaactgc gtgacggtga tctggtgttg attgacgcgg
    24541 gttgtgaata caaaggttac gctggcgaca ttacccgcac cttcccggtc aacggcaaat
    24601 tcactcaggc tcagcgtgaa atctatgaca ttgtgctgga gtctcttgaa accagcctgc
    24661 gcctgtatcg tccgggaact tccatgcagg aagtcactgg tgaagtggtg cgtatcatgg
    24721 ttagcggcct ggtaaaactc ggcatcctga agggtgatgt tgatgaactg atcgctcaga
    24781 acgcccatcg tcctttcttt atgcatggcc ttagccactg gttaggactg gatgtccatg
    24841 acgttggcgt ttatggtcag gatcgctcgc gcattctgga accggggatg gtactgaccg
    24901 tagagcctgg tctgtatatc gcgccggatg cggatgtgcc agaacaatat cgcggtatcg
    24961 gcattcgtat tgaagacgac attgtgatta ccgaaaccgg caacgaaaac ctcaccgcca
    25021 gcgtggtgaa aaagccggaa gaaatcgaag cgttgatggc tgctgcgaga aagcaatgag
    25081 cgtaatcatc gtcggtggcg gcatggcggg cgcgacgctg gcgctggcta tttcccggtt
    25141 aagtcacggg gcgctgccgg tacatttgat tgaagcgact gcgccagagt cacatgctca
    25201 tccgggcttt gatggacgag cgatagcgct ggcggcgggt acctgtcagc aactggcgcg
    25261 catcggcgtc tggcaatctc tggcggattg cgcaaccgcc atcactaccg tgcatgtcag
    25321 cgatcgtggt catgccggat tcgtcaccct cgccgcagaa gattaccaac tggcggcgct
    25381 ggggcaggtt gtcgaattgc ataatgtcgg gcaacggctg tttgcattgc tgcgcaaagc
    25441 acctggcgta acgctgcatt gtcctgatcg cgtggctaac gttgcccgta ctcagagtca
    25501 cgttgaagtg acgctggaga gtggcgagac gctgacgggc tgcgtgctgg tagccgctga
    25561 tggcacccat tcagcgttag ccaccgtgtg cggcgttgac tggcagcagg agccttacga
    25621 acaactggct gtgattgcca acgttgcgac ttccgttgcg catgaagggc gcgcttttga
    25681 acgctttaca caatatggcc cgctggcgat gttgccgatg tctgacggac gctgttcgct
    25741 ggtctggtgt catccactgg aacggcgcga agaggtactg tcttggagcg acgagaagtt
    25801 ttgccgtgaa ctccagtcgg cctttggttg gcgacttggg aaaattaccc acgctggtaa
    25861 acgcagtgct tatccgctgg cgttaacccg cgccgccaga cctattaccc atcgtaccgt
    25921 gctggtgggc aatgcggcgc aaactctgca cccgattgcc gggcaagggt tcaacctcgg
    25981 tatgcgagat gtcatgagcc ttgcggaaac cctgactcag gcgcaggagc gcggagaaga
    26041 catgggtgat tacggcgtat tgtgccgtta tcagcagcgt cgacagcgcg atcgcgaagc
    26101 aaccatcggc gtcacggaca gccttgtaca tctttttgcc aaccgttggg ggccgctggt
    26161 tgtcgggcgc aacatcgggc tgatggcaat ggaattattc accccagcac gcgatgtgct
    26221 ggcgcagcgc accctcgggt gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc
    26281 aaagtgttga tgtagccatt gttggcggcg gcatggtggg gctggcggtt gcctgcggct
    26341 tgcagggtag cggcttacgc gttgccgtac tggagcagcg cgtaccggaa cctctggcgg
    26401 cggatgcacc accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca
    26461 cccgtcttgg tgtctggcag gacattctct ctcgtagggc cagttgttat cacggtatgg
    26521 aagtgtggga caaagacagt tttggtcaca tttcgtttga cgatcaaagc atgggctata
    26581 gccatcttgg gcatatcgtt gaaaactcgg tgattcacta cgcgctgtgg aacaaagcgc
    26641 agcagtcgtc agatatcact ctgttggccc ccgcagaatt acagcaggtc gcctggggag
    26701 aaaatgaaac cttcctgacg ttgaaagatg gcagtatgtt aacggcgcgt ctggtgattg
    26761 gcgcggacgg cgctaattcc tggttgcgca acaaagccga tattccgctg actttctggg
    26821 attatcagca tcacgcgctg gtagcgacca ttcgcacgga agaaccgcat gatgcggtgg
    26881 cgcggcaggt ttttcatggc gaaggcattc tggccttttt gccgcttagc gatccgcatc
    26941 tttgctcgat tgtctggtca ctgtcgccag aggaagcgca gcggatgcag caggcaggcg
    27001 aagacgaatt taatcgcgcg ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg
    27061 ttgaaagtga gcgcctggtg ttcccgctaa cggggcgtta tgcgcgccag tttgccgcac
    27121 accgtctggc gctggtgggc gacgccgcgc atactattca cccgctggcg gggcaggggg
    27181 taaatctcgg ctttatggat gctgcagagc tgattgctga actgaaacgg ttgcatcgtc
    27241 agggtaaaga catcgggcag tacatttacc tgcgtcgcta tgagcgtagc cgtaagcaca
    27301 gtgcggcgct gatgctggct ggtatgcagg gattccgcga tctgttttcc ggtaccaatc
    27361 cggcgaaaaa attgctgcgt gatattggtc tgaaactggc tgatacgctt cctggcgtta
    27421 agccacaact tattcgtcag gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa
    27481 tatttctctt ctgctgttta tttgataccc atcacacttt catctcccgg ttttttcgcc
    27541 gggagatttt cctcatttga aataaactaa tttaacctcc gtt
//
LOCUS       DAZGAP010000054        27430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGAP010000054 DAZGAP010000000
VERSION     DAZGAP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.054"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..844
                     /gene="ygcG"
                     /locus_tag="ACHLAM_001919"
     CDS             <1..844
                     /gene="ygcG"
                     /locus_tag="ACHLAM_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO7853449.1"
                     /translation="FVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTRKLESIFN
                     NTQVQIAVLIIPTTKDETIEQYATRVFNNWRLGDAKRNDGILIIVAWSDRTVRIQVGY
                     GLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYSANPSESES
                     ASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFSDKTFS
                     IAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSGGGFSGGGGSSGGGGA
                     SGRW"
     gene            complement(904..2202)
                     /gene="eno"
                     /locus_tag="ACHLAM_001920"
     CDS             complement(904..2202)
                     /gene="eno"
                     /locus_tag="ACHLAM_001920"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO7853450.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2290..3927)
                     /gene="pyrG"
                     /locus_tag="ACHLAM_001921"
     CDS             complement(2290..3927)
                     /gene="pyrG"
                     /locus_tag="ACHLAM_001921"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO7853451.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4155..4946)
                     /gene="mazG"
                     /locus_tag="ACHLAM_001922"
     CDS             complement(4155..4946)
                     /gene="mazG"
                     /locus_tag="ACHLAM_001922"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO7853452.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5042..7276)
                     /gene="relA"
                     /locus_tag="ACHLAM_001923"
     CDS             complement(5042..7276)
                     /gene="relA"
                     /locus_tag="ACHLAM_001923"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO7853453.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7324..8625)
                     /gene="rlmD"
                     /locus_tag="ACHLAM_001924"
     CDS             complement(7324..8625)
                     /gene="rlmD"
                     /locus_tag="ACHLAM_001924"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO7853454.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8682..11438
                     /gene="barA"
                     /locus_tag="ACHLAM_001925"
     CDS             8682..11438
                     /gene="barA"
                     /locus_tag="ACHLAM_001925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO7853455.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11662..13002)
                     /gene="gudD"
                     /locus_tag="ACHLAM_001926"
     CDS             complement(11662..13002)
                     /gene="gudD"
                     /locus_tag="ACHLAM_001926"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO7853456.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13023..14363)
                     /gene="gudX"
                     /locus_tag="ACHLAM_001927"
     CDS             complement(13023..14363)
                     /gene="gudX"
                     /locus_tag="ACHLAM_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO7853457.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVGLDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14365..15717)
                     /gene="gudP"
                     /locus_tag="ACHLAM_001928"
     CDS             complement(14365..15717)
                     /gene="gudP"
                     /locus_tag="ACHLAM_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO7853458.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16152..16601)
                     /gene="yqcA"
                     /locus_tag="ACHLAM_001929"
     CDS             complement(16152..16601)
                     /gene="yqcA"
                     /locus_tag="ACHLAM_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO7853459.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     SGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16619..17401)
                     /gene="truC"
                     /locus_tag="ACHLAM_001930"
     CDS             complement(16619..17401)
                     /gene="truC"
                     /locus_tag="ACHLAM_001930"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO7853460.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17401..17730)
                     /gene="yqcC"
                     /locus_tag="ACHLAM_001931"
     CDS             complement(17401..17730)
                     /gene="yqcC"
                     /locus_tag="ACHLAM_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO7853461.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18352..18897)
                     /gene="syd"
                     /locus_tag="ACHLAM_001932"
     CDS             complement(18352..18897)
                     /gene="syd"
                     /locus_tag="ACHLAM_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO7853462.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18965..19813
                     /gene="queF"
                     /locus_tag="ACHLAM_001933"
     CDS             18965..19813
                     /gene="queF"
                     /locus_tag="ACHLAM_001933"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO7853463.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19925..21289
                     /gene="ppnN"
                     /locus_tag="ACHLAM_001934"
     CDS             19925..21289
                     /gene="ppnN"
                     /locus_tag="ACHLAM_001934"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO7853464.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21845..23134
                     /gene="sdaC"
                     /locus_tag="ACHLAM_001935"
     CDS             21845..23134
                     /gene="sdaC"
                     /locus_tag="ACHLAM_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO7853465.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23192..24559
                     /gene="sdaB"
                     /locus_tag="ACHLAM_001936"
     CDS             23192..24559
                     /gene="sdaB"
                     /locus_tag="ACHLAM_001936"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO7853466.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24670..25425
                     /gene="xni"
                     /locus_tag="ACHLAM_001937"
     CDS             24670..25425
                     /gene="xni"
                     /locus_tag="ACHLAM_001937"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO7853467.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25480..26628)
                     /gene="fucO"
                     /locus_tag="ACHLAM_001938"
     CDS             complement(25480..26628)
                     /gene="fucO"
                     /locus_tag="ACHLAM_001938"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO7853468.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26656..27303)
                     /gene="fucA"
                     /locus_tag="ACHLAM_001939"
     CDS             complement(26656..27303)
                     /gene="fucA"
                     /locus_tag="ACHLAM_001939"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO7853469.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6656 a         7317 c         6608 g         6849 t
ORIGIN      
        1 attcgtgtgt tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt
       61 tacggatctt acgagtagct taaactcaca agaaaagaaa gaactgacgc ggaagttaga
      121 atctattttc aataataccc aagtacaaat tgctgtatta attattccca caaccaaaga
      181 cgagacaatt gaacaatatg ctacaagggt ttttaacaat tggcgtttag gagatgccaa
      241 acgcaatgat gggatactga tcattgttgc ctggtcggat cgcactgtcc gcatccaggt
      301 aggttatggg ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa
      361 catgataccc gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc
      421 tttgaataac caactcactt cacaacacca atattcggct aatccttcag aaagtgaatc
      481 agcgtcttcc agcgatcatt actactttgc tattttttgg gtatttgcag tgatgttctt
      541 ccctttctgg ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gcagtgtctg
      601 tatttcagca atctatcttt tagatttatt cctgttctcc gataaaactt tttccattgc
      661 tgtattttcc ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact
      721 tcagaaaaga gcatcaggta gaagttatca ttcagacaac agcggttccg ctggaggctc
      781 aggcggcggg tttagcggcg gtggcggttc ttctggcggt ggcggcgcat ccggccgctg
      841 gtaactcaac cataaaaaac gccagcccga aggctggcat ttttaaatca gataaagtca
      901 ggcttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt cgcccagagc
      961 ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc tcatagaacc
     1021 agttttgatc tggcctgcag cagtaccaac agccaggtca gcgatagtag cgtcttcagt
     1081 ttcgccagaa cggtgagaga taactgcagt gtagccagcg tctttcgcca tcttgattgc
     1141 agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg agttagcgat
     1201 acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac
     1261 cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc agtcagattc
     1321 gtccagaccg tcttcgatag atacgatcgg atactgtttg gtcagttctt ccaggaagtg
     1381 agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt atttaccgtc
     1441 tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc ccagttcata
     1501 accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg aacccaggtt
     1561 cggcgcatag ccaccttcgt caccaactgc agtgttcatg cctttcgctt tcagaacttt
     1621 tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag ttttcgcgcc
     1681 aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct caccaccgtt
     1741 gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt tcagttcagc
     1801 gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt tagccagaga
     1861 tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat
     1921 catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg cctgagcgat
     1981 cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac gggatttgtc
     2041 gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg gagcagctgc
     2101 cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt taccacggga
     2161 gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt cctcaagtca
     2221 ctagttaaac tgaaactcca gacaaacaac gcgtacccag ggtacgcgtt gccgttctaa
     2281 cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca caaagcctgc
     2341 aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact ggcaagccac
     2401 gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc cggaacggcc
     2461 cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga cttcgtaacg
     2521 gtgacgatga cgctcaacaa ttgtcggcgc attgtacagc tggcgaacca ggctatcgtc
     2581 aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc tcttctcgct
     2641 acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca caactgggta
     2701 cttacagtct ggcacaaatt ccgtagagtt ggcgttctcc atgttggcaa catggcgagc
     2761 gtaatcaatt aacgccacct gcatacccag gcaaataccc agataaggaa tattgttctc
     2821 acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga aaccgccagg
     2881 tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga
     2941 atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc
     3001 gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac cgatggtgac
     3061 ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca gatttgcttc
     3121 cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag atttcaacag
     3181 gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt tttccggaac
     3241 attacagaac aatgcaatct tcgcacgttc gttcgccgga acggcgcgat ctgaacgaca
     3301 aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt gctgagtcgg
     3361 tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt gcataaacag
     3421 agtgtgctca cggccaattt caacagccat ctggcgaatc gcttcgagga acggcaggga
     3481 ttcgatatca cctacagtac cgccgatttc gaccagtact acgtcatgac cttcgccgcc
     3541 ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca cggttgcgcc
     3601 gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac ccgtggtgaa
     3661 gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca ggtccaggtc
     3721 ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc tcatagtacc
     3781 tggatcgacg ttgatgtacg gatccagttt catgatggtc acattgaggc cacgggcttc
     3841 aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata cgaccccgcc
     3901 ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg aaaagacgat
     3961 ggaataacca ggacgggaaa gcagtatacc cgaacatgac ctgtgccaca aactttcatt
     4021 atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc ccctttgggg
     4081 taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa atcgcgcttg
     4141 accacgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca gacttcttcc
     4201 attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat acgctccact
     4261 tcgcgaaaac gacgctcgaa tttttcgttc gctttttgca atgcgatttc cgcttttgtc
     4321 cctaaatgac gagccagatt gactgtggca aacagcaggt cacccatttc ctcctccagt
     4381 ttagcctggt cgacaacaac ctgccgcgct tcgtacatca cctcatcgat ctcttcgtag
     4441 actttatcga ctaccgggcc aagcgtcgtc cagtcgaagc caacgttggc gcaacgtttc
     4501 tggatttttt gtgcgcgcat taaggcgggt aaactactag ggatatcgtc cagcgccgaa
     4561 tgctgcgctt tttgcgcgcg ctcttcagtt ttgatttgct cccaacgagc aagaacttcg
     4621 cggctgtttc cggcagaacc gtcagcaaaa acatgtggat gacggcgttc taatttatcg
     4681 ctaatcgcag cgcaaatatc attaaaatca aagcgccctt cttcctgggc catttgtgcg
     4741 taaaacacca cctggaacag cagatcgccc agttcaccgc gcagatcgtc aaaatcttca
     4801 cgggcgatgg cgtccagcac ctcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca
     4861 aatgtctgct ctttatccca cgggcagccg ttttccgggt cacgcaggcg ctgcatgatg
     4921 gtgagcaaac ggtcgatttg attcattaga atgtcctgaa aattgcgggt ccgtcaggtg
     4981 taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca gctatgtaag
     5041 actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg agtttaccca
     5101 gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg gtcgccagtt
     5161 gctgtttggt gtcgctacgg ctggcaacgc caagtacgtt caccttctcg ttggcgagaa
     5221 tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg accaccagcg
     5281 aatatccggc ggagtagctc tcgccccata ccgcgtcaac aatgcgttct ggcgcatggg
     5341 agcgcagttc cgccaattgt tcgcaatcgg cgcggtgtac tgaaataccg cgcccctggg
     5401 taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg atgtggtgca
     5461 tcaggttacc gaccccttct accaccacac gaccgttatc tttactgcgg ttttgcggcg
     5521 tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg gcactcggct
     5581 tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc ccgccaccaa
     5641 tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga tgtttttctg
     5701 cttctttcag gctgatcccc agatgttcca gctcgtcgtc aagaatttgc cgcccagcca
     5761 gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa cgcccacggc
     5821 tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc ggctgtttct
     5881 gggtgataat ttcaatctgg tcgcccatct gcagctggta ggtgaacggc acaatgcgcc
     5941 cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg taagcgaagt
     6001 ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta aagacgtaca
     6061 ctcggtcgtc aaagacctga ctacgtactt cgtcgagcat ttcgccggaa tcagccatct
     6121 cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt cccgaacgtg
     6181 cgccgccagc agccgcgccc tctttatatt tccagtgcgc agcaacaccc aactctgcat
     6241 cttcatgcat ctgtttggtg cggatttgga tctcaacggt tttgccaccc ggccccagaa
     6301 ccacggtatg aatggactga taaccgtttg gtttcgggtt agcgacgtaa tcgtcaaact
     6361 catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa caatcctgta
     6421 aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag gcgaggttct
     6481 ttttctgcat tttgcgccag atactgtaga tgtgtttcgg acggccatac acttcagctt
     6541 taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg atatagtgtt
     6601 cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg gtcgggtgga
     6661 gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca agacggttcg
     6721 ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacgcgttca tcttccggcg
     6781 catcttttac ttcgcgcagg tgagcaatac gctccgccag tttgatgacc acgcagcgaa
     6841 aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg gaggaaacag
     6901 aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg ccgtgaataa
     6961 ggttaacgac cgacttaccg acgctctcac gcagcacatc ttcgctgact acgttggcat
     7021 cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt aatgtcgaga
     7081 ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga tgcccctgcg
     7141 tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc tggctggtaa
     7201 tacccagact tgcgatccat ttttccggat caaattcacc agccttattg atatgtgcac
     7261 ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat cggcaaatcg
     7321 taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt gtgggaacat
     7381 atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt cgctatcccg
     7441 agccagcgtt gcagggttac aggatacata aactatacga ataggttcca gttttataat
     7501 ttgctgcata acacctgcgg cacctgctcg cgccgggtcc aacaacactt tatcgaagcc
     7561 gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat aaaacgtcac
     7621 attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg ccggaacacc
     7681 ttctacaccc accacactag cagcttgtgt cgccaatggc agtgtaaagt tgcccatacc
     7741 gcagaacaga tccagtacgc gatcttcagg ttccacatcc agccattcca acgcacgcgc
     7801 taccattttc tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc taaaagttaa
     7861 gcgcaaccca tttgagtcat accagggcat ctcaccagag acggtttcga gtatctcact
     7921 atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca gtttttcgcg
     7981 atcagccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc tggttgcctg
     8041 taccagttca acatgaccaa gatggcgtat agcttgtaag ctgcccaggc atgccctgac
     8101 tttgggcagc aatgcttcaa gttggggcac taaaatggga cattgtttaa cgtcgacaat
     8161 gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg gtaagtagtt
     8221 cagacttaaa cgcgcgcgac ggcgatagcc ccagggaaca tcggcgatca cttcagagac
     8281 ttcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta aatccacgct
     8341 ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg gcgtttcgcg
     8401 ttccgggcta tcgcttaacc ggcgcacgac tttagcgcgg gcgtattgtt ttttatcttc
     8461 agtaacagta acttccgcgt tttcctgtgg caataatcca ggaataaata acgctttgcc
     8521 gttatgtcgt gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa cagttatgat
     8581 ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg agactttctc
     8641 aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac tacagcctgc
     8701 gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta ttgctgagta
     8761 tctttttcgt tgtgcatcgc tataacgact tgcagcgtca actggaagat gccggagcca
     8821 gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa aatcgcgaat
     8881 ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt cgcgcgattt
     8941 cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt gatccctcat
     9001 caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact cgtgacggcg
     9061 atattatgat cctccgcacg ccgattattt ctgaaagtta ctcccccgac gaatcgccca
     9121 gtagcgatgc caaaaatagt caaaatatgc tgggatatat tgcgctggag ctggatctta
     9181 aatcggttcg cttgcagcaa tataaagaga tctttatttc cagcgtgatg atgctgtttt
     9241 gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc ggtccgattc
     9301 gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc cgggtggaag
     9361 gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg gcaatgtcgc
     9421 tggcggctta tcacgaagag atgcaacaca atatcgacca ggcgacgtcc gatctgcgtg
     9481 agacgctgga gcaaatggag attcagaacg ttgagttgga tctggcgaaa aagcgcgccc
     9541 aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag ctgcgtacac
     9601 cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta acaccaacgc
     9661 agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca attattaatg
     9721 atgttctcga cttctcgaaa ctggaagccg gtaagctgat tctggaaagt attccattcc
     9781 cactacgcag cacgctggat gaagtcgtta ccctgctggc acattcttct cacgataaag
     9841 ggctggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc ggcgacccac
     9901 tacgattaca gcaaatcatc actaacctgg tagggaatgc aattaaattc accgagaatg
     9961 gcaacatcga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg cagattgaag
    10021 tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc ttattccagg
    10081 ccttcagaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg ctggggctgg
    10141 tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat agcctgccga
    10201 atcgtggttc aactttctgg ttccacatta atctcgatct gaacccgaac attattatcg
    10261 aagggccatc cacccagtgc ctcgctggta agcgcctggc ctatgtcgaa ccaaactccg
    10321 cagcagcaca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg gtttatagcc
    10381 caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttattaggc atagcggtga
    10441 ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcagta tcgatgaccg
    10501 atttcctgat gctggcactt ccttgccatg cacaagtcaa tgctgaaaaa cttaagcaag
    10561 atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg cctgccctga
    10621 cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa agtaagctgg
    10681 caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc ggcgcattgc
    10741 tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggcg gttgaacggg
    10801 cgaaacagat gccgttcgat ttaatcttaa tggatattca aatgccggac atggacggca
    10861 ttcgggcatg tgagctcatc caccagctcc cgcatcagca gcaaacgccg gttatcgcgg
    10921 taacggcgca tgcgatggcc gggcaaaaag agaaactgct tggcgcgggg atgagtgatt
    10981 atctggccaa accgatcgaa gaagagcgat tgcataattt gctgttgcgc tacaagcctg
    11041 gcagcggtat ttcctctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg gtcaacccga
    11101 atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc gatttagcgc
    11161 gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa gttgaggaac
    11221 agctggttgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg catggcagtt
    11281 gcggctatag cggtgtaccg cgtatgaaga atctctgcca actgatcgaa caacagctac
    11341 gtagcggtac taaagaagaa gatttagagc cggagctgct ggaactgttg gacgagatgg
    11401 ataatgtagc gcgcgaagcc agcaaaattc tcgggtaatg gacgtcttgt cgagtagcaa
    11461 ccggattttt atgacgctgg tttcagattg gtgaaaagtg cctgtgttta tgccggatgc
    11521 ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta aaaaatgatg
    11581 taaccctgat acgtagcgca acaggcagtt tggcatcagt ctcaaaccgg ctccagatag
    11641 agccggtttt ggttttctgt cttaacgcac catgcacggg cgcttgttat caaacgtcca
    11701 gccggggatc aggtactgca ttcccatcgc atcgtctcgc gcaccaagcc catgtttctg
    11761 atacagctcg tgggctttca tcacctgatc catatcgatt tctacgccca atcctggttt
    11821 ttgcggcacc tgtaccagcc cgcctttgat ctcaaacggt tctttggtca gacgctgatt
    11881 gccttcctgc cagatccagt gcgtatcaat agcagtaatt ttacccggtg cagcagcggc
    11941 tacatgagta aacatcgcca gggaaatatc gaagtggttg ttagagtgtg aaccccaggt
    12001 caggccaaat tcatggcaca tttgcgccac acgtaccgaa ccttgcatcg tccagaaatg
    12061 cggatccgcc agcgggatat caacggattg taacgagagc gtatggccca tctgccgcca
    12121 gtcggtggcg atcatattgg ttgcggtcgg caggccagtc gcgcgacgga actctgccat
    12181 cacttcacgc ccggagaaac cttgctccgc accacacgga tcttctgcat aagccagcga
    12241 acctttcagg tatttaccaa ttttaatcgc ttcgttcagc gaccaggcac cgttaggatc
    12301 gagggtaata cgcgcctgtg ggaagcgttg cgccagtgcc acaatagatt ctgcttcttc
    12361 ttccccggcc agtacaccgc ccttcagttt gaaatcgttg aagccatatt tttcatatgc
    12421 cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct tcttcgtgac gcaggcgata
    12481 ccagtcacat ttatcatccg gctggctttg atacggcagc ggtgtggctt tgcgattacc
    12541 gacgaagaac agataaccga gcatttcgac ttcgctacgc tgttgaccat cgcccagcag
    12601 cgatgccacg ttaaccccca gatgctgccc cagcagatcc agcattgccg cttctatccc
    12661 ggtaaccaca tgaatagtgg tccgcaggtc aaatgtctgc aaaccgcgcc cgccggcatc
    12721 acgatcggca aatgtattac gcaccagcgt cagaacgttt ttgtattcac ccagcgtttt
    12781 acccaccacc agcggaatcg catcttccag cgttttacgg attttctcgc cgccgggaat
    12841 ttcccctacc ccggtgtgac cagaattatc tttgataatc acaatattac gcgtgaagaa
    12901 cggtgcgtgt gcaccactca gattcatcag catactgtca tggcccgcca ccgggatgac
    12961 ctgcatttca gtaacaacag gcgtcgaaaa ttgagaactc atctttatgt cctttttcaa
    13021 aatcaatgac ggccgaaaac gggacgttta cggtcaaagg tccagccggg gatcaggtac
    13081 tgcatcgggc ccgcgtcgtt acgcgcaccc ccaggcagac gtttataggc ctcatgcgcc
    13141 ttttgtacct gctcccagtc cagtcccacg cccagaccgg gcgcatcagg aacggcaatt
    13201 tttccgtttt taatctccag cggatttttg gtcaggcgac aatcgccctc ctgccaaatc
    13261 cagtgggtat cgatagcggt aggattaccc ggtgccgccg cacccacatg ggtaaacatc
    13321 gccagagaga tatcaaagtg gttattggaa tggcagcccc aggtcagccc ccagtcgtcg
    13381 caaagttgcg ccacgcggac tgcaccggaa agcgtccaga agtgcggatc ggcaagtgga
    13441 atatctaccg cattgagcat caccgcatga cccatttcgc gccagttggt ggcgatcatg
    13501 ttggtcgcga cgggcaagcc ggtcgcccgt cgaaactccg ccatcacttc acgaccggag
    13561 aaaccctgct cagcgccgca cgggtcttcc gcataggtaa ggacatcatt cagccctttg
    13621 cacagagaaa tggcttcatc aagcagccag gcaccattgg gatcaacggt aatccgcgca
    13681 tcagggaagc gtttcttcaa tgcacgaaca gtgtcgattt cttgctcgcc aggtaacacg
    13741 ccacccttaa gtttgaaatc tttaaagcca tagcgatcct gtgaggcttc cgccagacgc
    13801 acaacggctt cgctgttcat cgctttctga tgccgcaact gataccactc atggttgccc
    13861 ggcgtatttt ccagataagg aagatcggtt ttggtccgat caccgatata aaacagataa
    13921 ccgagaacgg taatagcatc acgttgcttg cctggcccta acagttcgca gaccggaaca
    13981 ttcagcgcct gacctaacag gtcaagcaag gcggcttcca gcgccgccac ggcattaacg
    14041 cgcaattcaa aagtccaggc acctttgccg aaggtatcaa aatcggctgc ctgattacct
    14101 ttatgcacct gctgaaccac tttattcagg cgcgcaactt cctggcccag caccatcgga
    14161 atagccttga ccagtgtttg ataaatcacc tctccgcccg gcgcttcacc aatgccggta
    14221 tgcccggcat tatcggtgag taccacaata ttgcgggtga aatatgcgtt atgtgcacca
    14281 ccaatattaa gcaacatgct gtcatgtccg gcgaccggaa tgactttcat atcagtaata
    14341 acaggactgg attgtgtcgt catcttattg ccccgcgaca ggtttcagct caatacgctt
    14401 gatatcgccc accagcacca ggtagctgag caccgcgatt aaggcatgaa caccaacata
    14461 aatcagcgcc ccattaaagg agccagtcgt gccaacgata taaccaattg cgattggcgt
    14521 gacgatgcca gaaatgttac caaacatgtt aaacaggcca ccggaaagac cgctgatctc
    14581 ttttggcgca gtatcagcca ttacagccca acccagcgca ccgatcccct taccgaagaa
    14641 ggccagcgcc ataaagccga tgatcatcca ctcaacgttg acgtagttgc agaacaccat
    14701 caccatcgac aacaacatgc ccatcacgat cggtgtttta cgcgcaatgt tcagtgaacc
    14761 cgtgcgacgc atcagccagt cggaaataat cccacccagc acaccgccga taaaaccgca
    14821 aaccgccgga acggaagcca caaagcccgc tttcagaatc gacatcccgc gcgcctgcac
    14881 cagataaacc gggaaccagg taatgaaaaa gtaagtcagg gcgttgatac agtactgacc
    14941 gatataaacg ccgatcatca tccgtgaccc gagcagctgt ttgatctgcc cccacttcac
    15001 gctgaacggc actttaactt tggtgttttg ctgatccata ttgatcagcg cgccacccgc
    15061 ggcgatgtac tccagctctt tctgatttac gcccggatgt tgatttggct cgtggatgac
    15121 tttcaaccag ataaagctga tgacaatgcc cagaccgccc ataaagaaga agacgtgtga
    15181 ccagcccact tcatgagtta gccagcccat aatcggcgcg aagataaccg ttgcgaagta
    15241 ttgggcggag ttaaaaatcg acaccgccgt tcccctttcc tgcgccggaa accaggccgc
    15301 aacaatgcgg ctgttgccag ggaaagatgg cgcttcagca agcccgacca ggaagcgcag
    15361 tgtaaacagg gcaacgataa tgccgaatcc actaaagata tcgacgaagc cttgcagcaa
    15421 ggtaaacatc gaccagataa agatcgacca gaagtagacg cgttttgaac caaaacgatc
    15481 cagcaaccag ccaccaggga tctgcccgat aacatacgcc catgagaagg cagagaacac
    15541 atagcccatt cccacgggat caaggccgat atctttggcc atttccgaac cggcaataga
    15601 aagcgtagcg cggtcgccgt agttgaagga tgtgacgata aacaacatca ccactatcca
    15661 gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc tgacttaaag aactcattgt
    15721 tgcactcctg aaaattcgcg ttagccacgc tcactctgga ctgcgacatc gccaggaaat
    15781 cagaggtgac gtagggtgtt ttttgccgtt tttataggtc gttcgccgaa tacggcgcgt
    15841 gtttatatct ggcaatagca gtataaaaag cgcgccatag cggctcaccg tgcaacaaca
    15901 caacattaat gcgttcaatg aggcccgatt ttggcattag ccctggacgg tggaatccac
    15961 ttcacggaaa tgaaaacaag aacaagaaag gaagggttaa aacgaagaaa taaagaagag
    16021 tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa aatccgcgct atgaagcagt
    16081 ttttactggc atttgcctga aaagattcga ttcagcaccg ctaaaacggc atttaccgct
    16141 cgctgaacat atcaggacaa catcgtgccc caatgttcga cccacggatt tgattccgtt
    16201 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    16261 tgttcctgca acaaggcatc gaactgtttg ccactattgc agaaattcac ataactactg
    16321 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagggt atctttaatt
    16381 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    16441 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    16501 gttgctttgt ggccctgcgc ggtcagaatt gcttccgctt cttccgccac taacagcgaa
    16561 tttccgtaca tagtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    16621 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    16681 gaagcagacc ccgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    16741 cgtgaatagt cagtggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgca
    16801 gcattaaccg ctgaaggcca aaatgttcag caccgctgcg attctggcgt aaatcaccat
    16861 gtttgctgtc gccaataatc gggtggcgca aatgggcaag atggcgacgg agctggtgct
    16921 tacgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    16981 tcgctaccag catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    17041 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    17101 ccagcaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    17161 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    17221 acacaccaga agtgggtcgg tccagacgat gagcagtaaa cacatgctgg cctatcaggt
    17281 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagctg cggtgaacca
    17341 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    17401 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc agctcagcca gaataagcgc
    17461 gcgttgcgga tgatcagtcg ccagcgccat ttcataatag ggtgcaacgg caaaagcgcc
    17521 cggtaacggc tggttgttat ctaacaaatc gtgcattcgc gggatcagca cccactgcaa
    17581 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactgttaa attgatgcgg
    17641 ctggggttta tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    17701 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    17761 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    17821 agcattccgg ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    17881 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    17941 atcctgaagt gttcctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    18001 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    18061 ttccctgaaa caccatcctg gtgtgtcctt cagaagtgtc atcatcctga tgttcacttc
    18121 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    18181 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aagcaacatg
    18241 ttgtttttaa atgagaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    18301 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag
    18361 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    18421 tcctagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    18481 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    18541 atgaccaatc agattttcct gcacccgacg gaaatcgtct tcactccagg tttgcagcaa
    18601 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    18661 ggtatgaatc gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    18721 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    18781 cggtgaagga acgccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    18841 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    18901 tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg
    18961 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    19021 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    19081 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    19141 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    19201 acttgattac accagcgcaa atctgattga gtcgaagagt tttaagctct atctcaacag
    19261 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    19321 aagcacttgc gctcagggta aggttagcgt ggcgttatat cgtcttgatg aactggaagg
    19381 ccaaccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    19441 ctatgaattc actactgact atctggagaa tgccaccagt ggcgaaaaag tagtagaaga
    19501 gacgctcgtc agccacctgc tgaaatcaaa ttgcctgatc acccatcaac cagattgggg
    19561 atcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    19621 ggtttctttc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    19681 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    19741 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    19801 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    19861 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    19921 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    19981 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    20041 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    20101 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgataaatcc
    20161 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    20221 ggttctgcgt gacattctct tcgtttacgg gcaaatccat aacaccgttc gttttcccaa
    20281 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    20341 tcgcgcactg catgtcggtg aagcgccaaa tatggtggtc tgctggggcg ggcactcaat
    20401 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaat cagctgggcc tgcgtgagct
    20461 gaatatctgc accggctgtg gtccgggagc gatggaagcg ccgatgaaag gcgctgcggt
    20521 cggacacgca cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    20581 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    20641 aaaacgtctg gaagcgtttg tccgtatcgc tcacggtatc attatcttcc ctggcggtgt
    20701 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    20761 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    20821 ggacgagttt gtcgtgcata cattgggtga aaacgcgcgc cgccattacc gcatcatcat
    20881 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    20941 tcgccgcgac acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    21001 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    21061 acccgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    21121 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttataaaa tcaacggcga
    21181 taaagagatt atgcgtcgta tggatgacct gctacagggt tttgttgccc agcatcgtat
    21241 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctccctcccc
    21301 cggtgacgtt tgttgccggg ttttctcgtt tttggtcaca tacccatcaa tttatttcat
    21361 ttgttatatg aatgtttctt accaccctca tgcgataaat ttcattacag ttaatatgtc
    21421 atacaattta tgttgtaacg caaacgtttc cctattttca taaaccacat tttatcgcta
    21481 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
    21541 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
    21601 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagtagatt
    21661 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
    21721 aaattattac ggcggtaaat gattaagcca tcgcctatag acagatttca tttttacggt
    21781 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
    21841 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
    21901 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
    21961 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
    22021 tgcgttcccg atgacatttt ttgctcaccg cggtctgact cgcttcgtac tgtctggtaa
    22081 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
    22141 actgattacc ctgctctact tcttcgctat ctacccgatt ctgctggttt atagcgtggc
    22201 aatcaccaat acagttgaaa gcttcatgtc tcaccagctg ggtatgacac caccgccgcg
    22261 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct tcggtgagca
    22321 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
    22381 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
    22441 tgcatctgca accggaaacg gtctttggat gaccctgtgg ctggcaattc cggtaatggt
    22501 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
    22561 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
    22621 gctgaccgta atgttcttcg tcttcagctg tgtactgagc ctgactccag cagacctggc
    22681 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcgcc
    22741 gatcatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
    22801 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
    22861 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
    22921 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
    22981 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
    23041 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc
    23101 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
    23161 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
    23221 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
    23281 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
    23341 cggctcgctc tctctgaccg gtaaaggcca ccacactgat atcgccatta ttatgggcct
    23401 ggcgggtaac ctgccggata ccgtggatat cgattccatc cctggtttta ttcaggatgt
    23461 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
    23521 tcagtgcatg aacttccatg ccgacaacct ttctctgcat gaaaacggta tgcgcattac
    23581 cgcactggcg ggcgataaag tcgtttacag ccagacttac tactctatcg gcggtggctt
    23641 tatcgttgat gaagaacatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
    23701 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
    23761 cctgatgatg aaaaacgagc tggcgctgca cagcaaagaa gagctggaac agcacctggc
    23821 gaacgtctgg gaagtcatgc gtggcggtat tgagcgtggt atttctaccg aaggcgtgtt
    23881 acctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
    23941 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
    24001 agtgaacgaa gagaatgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
    24061 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa
    24121 ctcactggct cgttatctgc tggtagccag cgccattggt tctctttata agatgaacgc
    24181 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
    24241 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtgtgca tcgcggcgga
    24301 aatcgccatg gagcataacc tcggtctgac gtgtgacccg gttgctggac aggtgcaggt
    24361 accgtgcatc gagcgtaacg ccattgcggc agtaaaagcg gtgaacgccg cgcgtatggc
    24421 gctgcgccgt accagcgagc cgcgcgtctg cctcgataaa gttatcgaga ccatgtacga
    24481 aacaggtaaa gatatgaacg ccaagtaccg cgaaacctct cgcggtggcc tggcaatgaa
    24541 gatcgttgcc tgcgattaat ctctcccgga ggcctcgttt tgcgaggcct cttcccgttt
    24601 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
    24661 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat tcgtcgcatt
    24721 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc acgcgctcga tcagctcatt
    24781 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
    24841 tggcgtcatc agcgtttacc agattacaaa gcgggtcgac cgccaatgcc ggaagagttg
    24901 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtcc
    24961 gccagcggca acgaagccga tgacttagcc gccacgctgg cggtcaaagt gacacaggcc
    25021 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
    25081 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
    25141 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
    25201 aaggtaccgg gtgttgtggg gatcggacca aaaagcgcca cgcagctgct ggtcgagttt
    25261 aagagtctgg aagggatata tgagaatctg aatgcggttg ccgaaaagtg gcgcaaaaaa
    25321 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
    25381 ttgcatatcg acggcaattt acagcaattg cggttggtaa ggtaacggcg tgccggatac
    25441 ggcgcaaacg tcgcatccgg cattatcaca tcagcgcatt taccaggcgg tatggtaaag
    25501 ctctacaata tcctcaagcg ttgcttcacg cgggttgcca ccggtacaaa catcatccag
    25561 tgccgcctgc gccagtgtcg gaatatcttc cttacgtaca ccaacatcac gtaaatgtgg
    25621 cggaataccg acatcacggt tgagagcaaa caccgcttca acagcggcat tacgcgcctc
    25681 ttccaggctc ataccttcca ctttcacgcc cataacgcgt gcgatatcgc ggtacttctc
    25741 accggtaaag tcagcgttat aacgcataac atgcggtaac aggatggcgt tcgcaacacc
    25801 gtgtggcgtg ttgtaaaacg cgcccagtgg atgcgccata ccatgcacca accctaaccc
    25861 aacattcgag aatcccatac ccgcaacata ctgcccgagc gccatttctt ctccggcatc
    25921 cttatcacca gcaaccgatc ctcgcagcgc cccagcaatg atttcaatcg ctttaatgtg
    25981 cagcgcatcg gttagcgccc acgcgccacg ggtaatatac ccctcaatag catgagtgag
    26041 cgcatcgaca cccgtcgcag ctttcagcgc tggaggcata ccatccatca tgtcagcgtc
    26101 aataaacgcc acctgcggga tatcatgcgg atcaacgcaa acaaacttgc gccgtttttc
    26161 ttcgtcagtg atcacgtagt taatggtcac ttctgccgca gtacctgctg tggtggggat
    26221 tgccagaatc ggtacactgg gtttattggt cggggaaagc ccttccaggc tacgcacatc
    26281 ggcaaactcc gggttgttgc tgataatgcc aatcgcttta caagtatcct gtggagaacc
    26341 gccaccaata gcgatcaggt aatccgcgcc gctattctgg aatacaccga gcccttcttt
    26401 gacgacagta attgttgggt tcggcactac gccgtcgtaa atcgcccatg ccagccctgc
    26461 agcatccatc ttatcggtca cttttgccac cacgccgcat tgcaccagcg ttttatcggt
    26521 gacgatcagc gccttctgat aaccacggcg tttcacctca tcggttaaag acccaacagc
    26581 cccccgacca aaccatgccg tttcgttcag aatcattctg ttagccatca tccttctcct
    26641 tgttgcttta cgaaattact cttcaatccg taacccgtag gttttgaatt tctccagcac
    26701 tacggcaatc tcttcatcgc tcagcactgg caccgggtcc gtaatcgcca gggtcgtcag
    26761 gtaaagttgt gccagcactt caacttcatg cgccagccat aacgctttct ccagattcgc
    26821 ctcacaagcg ataagcccat gatgttgtaa caaagttgcc ttacgatttt tgagagccag
    26881 cgcaacatgt tcagaaagtt cgcgtgttcc aaaggtcgca taaggcgcgc aaggaataga
    26941 attaccgcca gccgccgcaa tcatgtagtg aatcgcgggg atcggtcggt taagaatgga
    27001 aactgccgta caatgaacgg catgattgtg aacaaccgcg ttggcatccg gtctgctttg
    27061 ataggccgcc atatggaaac gccactcgct tgaggggagt tttccttcct catgtttgcc
    27121 gttgccatca ataaagacaa tatgcgactc cgtcagtttt tcatatggaa tgcctgtagg
    27181 cgtaatcagc atcccatcct gataacgtac actgacgtta cccgctgtcc cctggttcag
    27241 tcccaggcgg gtcatttcca ggcaagtgtc aataatctga cgagcaagtt tatttcgttc
    27301 cattagctac ctctctctga ttcaaaacag ggcaataatg ttgttccttt cacactattg
    27361 aattagccgt ttaattaccc accatcttct tcctgattaa ccagaaagaa attcacaagc
    27421 ttatattttg
//
LOCUS       DAZGAP010000055        27246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGAP010000055 DAZGAP010000000
VERSION     DAZGAP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.055"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..92)
                     /locus_tag="ACHLAM_001940"
     CDS             complement(<1..92)
                     /locus_tag="ACHLAM_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO7853470.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKT"
     gene            282..524
                     /locus_tag="ACHLAM_001941"
     CDS             282..524
                     /locus_tag="ACHLAM_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853471.1"
                     /translation="MNNESEKAMWRCKPVDNPWTLDSLLKKTISAVLPASDKVSHQNK
                     NKLRNNEFIKIEHPCSKSESRIDGDYIISGGTEYRL"
     gene            complement(572..2077)
                     /locus_tag="ACHLAM_001942"
     CDS             complement(572..2077)
                     /locus_tag="ACHLAM_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO7853472.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPQEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
     gene            complement(2084..2425)
                     /locus_tag="ACHLAM_001943"
     CDS             complement(2084..2425)
                     /locus_tag="ACHLAM_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853473.1"
                     /translation="MSRIYGRMKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(2430..3850)
                     /locus_tag="ACHLAM_001944"
                     /pseudo
     CDS             complement(2430..3850)
                     /locus_tag="ACHLAM_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
     gene            complement(3864..4838)
                     /locus_tag="ACHLAM_001945"
     CDS             complement(3864..4838)
                     /locus_tag="ACHLAM_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO7853474.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCTVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            complement(4835..5230)
                     /locus_tag="ACHLAM_001946"
     CDS             complement(4835..5230)
                     /locus_tag="ACHLAM_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO7853475.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            complement(5366..5998)
                     /locus_tag="ACHLAM_001947"
     CDS             complement(5366..5998)
                     /locus_tag="ACHLAM_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7853476.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            complement(6190..6639)
                     /locus_tag="ACHLAM_001948"
     CDS             complement(6190..6639)
                     /locus_tag="ACHLAM_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853477.1"
                     /translation="MLKKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQLS
                     DVKDAAEDVKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKMAEVSEAAQSGLS
                     EVKDAVEEVKTQAAGKVAEVSEATQSGLSDVKDTAPEKGAEISVTEE"
     gene            complement(7025..7213)
                     /locus_tag="ACHLAM_001949"
     CDS             complement(7025..7213)
                     /locus_tag="ACHLAM_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO7853478.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQAECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            complement(7591..7968)
                     /locus_tag="ACHLAM_001950"
     CDS             complement(7591..7968)
                     /locus_tag="ACHLAM_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7853479.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(7973..10669)
                     /locus_tag="ACHLAM_001951"
     CDS             complement(7973..10669)
                     /locus_tag="ACHLAM_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO7853480.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            complement(10807..11199)
                     /locus_tag="ACHLAM_001952"
     CDS             complement(10807..11199)
                     /locus_tag="ACHLAM_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO7853481.1"
                     /translation="MTVMVAVFILSGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            complement(11235..12722)
                     /locus_tag="ACHLAM_001953"
     CDS             complement(11235..12722)
                     /locus_tag="ACHLAM_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO7853482.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNNSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            complement(12712..13620)
                     /locus_tag="ACHLAM_001954"
     CDS             complement(12712..13620)
                     /locus_tag="ACHLAM_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO7853483.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSWGEVKHAD"
     gene            complement(13620..14282)
                     /locus_tag="ACHLAM_001955"
     CDS             complement(13620..14282)
                     /locus_tag="ACHLAM_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO7853484.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            complement(14279..14641)
                     /locus_tag="ACHLAM_001956"
     CDS             complement(14279..14641)
                     /locus_tag="ACHLAM_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO7853485.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGGIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            complement(14654..14968)
                     /locus_tag="ACHLAM_001957"
     CDS             complement(14654..14968)
                     /locus_tag="ACHLAM_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO7853486.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            complement(15045..15287)
                     /locus_tag="ACHLAM_001958"
     CDS             complement(15045..15287)
                     /locus_tag="ACHLAM_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO7853487.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            complement(15287..15628)
                     /locus_tag="ACHLAM_001959"
     CDS             complement(15287..15628)
                     /locus_tag="ACHLAM_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO7853488.1"
                     /translation="MFSFPASSCRAGRLAGAVGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVIGDYRRERP"
     gene            15741..16307
                     /locus_tag="ACHLAM_001960"
     CDS             15741..16307
                     /locus_tag="ACHLAM_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853489.1"
                     /translation="MDEGKQPGTPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVP
                     RVRELITWYEKYAPFTELPEDIKQQLQNIISLPDVSESEVQLLAMSLHDIYNSCQKKE
                     KLARRINYVLGAVSIASFCISLFQILFK"
     gene            complement(16691..17452)
                     /locus_tag="ACHLAM_001961"
     CDS             complement(16691..17452)
                     /locus_tag="ACHLAM_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO7853490.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSAPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLYEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(17445..19541)
                     /gene="traD"
                     /locus_tag="ACHLAM_001962"
     CDS             complement(17445..19541)
                     /gene="traD"
                     /locus_tag="ACHLAM_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO7853491.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTERSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(19538..20326)
                     /locus_tag="ACHLAM_001963"
     CDS             complement(19538..20326)
                     /locus_tag="ACHLAM_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853492.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGERMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEAQLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(20323..20805)
                     /locus_tag="ACHLAM_001964"
     CDS             complement(20323..20805)
                     /locus_tag="ACHLAM_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853493.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(20802..21395)
                     /locus_tag="ACHLAM_001965"
     CDS             complement(20802..21395)
                     /locus_tag="ACHLAM_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7853494.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            complement(21392..21910)
                     /locus_tag="ACHLAM_001966"
     CDS             complement(21392..21910)
                     /locus_tag="ACHLAM_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO7853495.1"
                     /translation="MKRERLFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(21907..22587)
                     /locus_tag="ACHLAM_001967"
     CDS             complement(21907..22587)
                     /locus_tag="ACHLAM_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7853496.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEELK"
     gene            complement(22566..23285)
                     /locus_tag="ACHLAM_001968"
     CDS             complement(22566..23285)
                     /locus_tag="ACHLAM_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO7853497.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPGMLPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(23289..24038)
                     /locus_tag="ACHLAM_001969"
     CDS             complement(23289..24038)
                     /locus_tag="ACHLAM_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853498.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(24035..24634)
                     /locus_tag="ACHLAM_001970"
     CDS             complement(24035..24634)
                     /locus_tag="ACHLAM_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO7853499.1"
                     /translation="MKKRFILSGLTVCLLMAGCQGTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(24820..25740)
                     /locus_tag="ACHLAM_001971"
     CDS             complement(24820..25740)
                     /locus_tag="ACHLAM_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7853500.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEEREKALQENYAS
                     ARRMQQMGISSEKIKEILQFSDDDLRNALR"
     gene            complement(25789..26349)
                     /locus_tag="ACHLAM_001972"
     CDS             complement(25789..26349)
                     /locus_tag="ACHLAM_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7853501.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(26365..26568)
                     /locus_tag="ACHLAM_001973"
     CDS             complement(26365..26568)
                     /locus_tag="ACHLAM_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853502.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(26659..27126)
                     /locus_tag="ACHLAM_001974"
     CDS             complement(26659..27126)
                     /locus_tag="ACHLAM_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO7853503.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT         6326 a         8464 c         6287 g         6169 t
ORIGIN      
        1 gttttgatgg tagaacctat tttaaagtac tcatccacac tcgtgtcatg gaacactact
       61 gtgcggtatt caggatggat atttggtttc atctctcttc actcatgtta taacataaca
      121 ataagataca ttctcagcta gatcatttca acagaatccc cgagcccaag ggtaatgtgg
      181 catcgcagcc tctcatcaaa tggaagacat gtgattgcac atttatcata caagacgtat
      241 gataacactg ttttatttat cctgctgccg agggtataga tatgaataac gaatctgaaa
      301 aagcaatgtg gcgttgtaag cctgtggata atccatggac gttggattca ttactaaaaa
      361 agactatttc tgctgtttta cctgcatcag acaaagtttc acaccaaaat aaaaacaaat
      421 tacgtaataa tgaattcatt aaaattgagc atccatgctc aaagtctgaa tccagaatcg
      481 atggtgacta catcattagt ggaggaacag aataccgttt gtaattaagc aggagaattt
      541 tctgtaaatt ctcctgtaac ttttttactt cctattttat acctcgatta gcaatacttc
      601 ctgcactcga agctgcacct ttcgcttccg aaataccatt cggtaatcct cgactgattc
      661 catcgccaat atgaacacca gcccaggaca acgctcccag ccacactgcc ggcaagacga
      721 gaaacatcgt ccccatcacc agattcatta tcagatcatc cgatgtgttc tggaatccca
      781 tcatattgaa ccgggtatgt gtatccgaac tgtacagcgc cgtcagcatc cagctgtcaa
      841 gccaccgtgc aagctcccac cagaacgtca gaaagtgtac cgcaaacacc acaaacgtca
      901 gtgtgattaa cgtcgacagt tcatatgccg caaacgccag aatcagcggt atcatgatgt
      961 aaatcgccat cagtattatc gactgtacca tcggcagtgc ctgccgcatt gcatccagtg
     1021 ccggaaacgc acccagtgct gctaccgcac tgcccgccgt tcccgcaatc cgggccaccg
     1081 cattcatggt cgtcatatct gcattgccac catagccacg atacacctct cccccctgag
     1141 agagcgtcag gtttgccggg ctcaccagac gcttgatcac ctcctcctta taatccacct
     1201 gactactgcc aatcatcttc attgcagctg acagacgtaa ccagtaatga tcactgacct
     1261 cctccatgac ccgttttttc agccccacac tactgtcact ccaccactcc cggcatgtcg
     1321 gatagcctcc ccgccccgta tccggtcgtc cgctgtcacg tgctgtattc cacgggaacg
     1381 cagcccgcgg catctttgac tgcagcgtcc cgtaatcccc ctgcatgaat gtggcgctcc
     1441 ctatccagga gatatcatca agcacttttt tatctttact ctgcccctga tcacgctgtt
     1501 tccacatata cagtgccagt gaataacagt cgttcgtaaa gtcctgcagc tcatcagcaa
     1561 gtgccctgtt ttctataaac gttcgctgga tctcaaaacg caactggcgc aaatcaggcc
     1621 ggcacggaat cgaagccact gccgcctggg tgacaccttt cgacaattta tgaaccacca
     1681 cccaccatac cggggcagca gcagtctgat cccctatact gctgatcacc ccactgtacc
     1741 ccgtctctcc gggctcttga ggtgtccata ccccacaggt ctgtgccctg gatttatcga
     1801 atgacagcgt atccagcgtc acctgtacca gaggaacaca gcaggatatc atcacaaaaa
     1861 aagcaccata gagaacatgc tcaatacgcg gcagggaaag catccctttg tttccctcat
     1921 ccgcaccttc ttccctgact ttcagccaga tcccgatgat cctgaatgcc agtggcaggg
     1981 caaacagtcc tgtgctgatc aacacattcc acagaccgtt attcaccacc cagcccagca
     2041 gagtcagaaa atattccaga tagttatttg tggtcatgat tgctcaacgc cccggctcag
     2101 caaaaatggc gtattcacac agcaggataa acagaacact gactgtggcc atacgcagaa
     2161 aagaagcccg gtattctgcc ctgaaccccg gcgcatgcca gatcctccac cccagccatc
     2221 cgatcgcact gtaaagacac agacgccatc ccagccagcc ataacgtgtc tcatacatcc
     2281 actcccgcag ggccgcaaat ccttctgcgt gacgtatacc ggtatcggcc agccagaggg
     2341 aaaacacggt aatccctgtc agcaacagcg tatacagggc gaataccttc atcctctttt
     2401 tcatccggcc ataaattctg ctcatcctct cactccttat ccggggcatc gagagaatgg
     2461 atacggctgt cagcattgtc agcctgctgg atcatcgggt tattccccac acgctcgtta
     2521 tcccgttcaa tgatggtcag aacactgtta cgcgccagat cccgcttcag ctccatctcg
     2581 tttttcagcg ccataatttc ccgatccagc gcctcaatac ggcgttcccc ctcttcagtg
     2641 cagcaggctg agctgcagca tacggctcgg ccatccccgt gagcagcatc ctgcgcatca
     2701 gcagcgccgt ttcaacggta tccgccatgg ccagttctcc cgccagacgc gacgtcaggg
     2761 caccattgtc cggatcacgc tgcagggcac ggatcactcc cgctgtcaca gacagactcc
     2821 cggctttcag ttttgccaga ttcccggctg tcggcttgtc acgaccattg accatacctg
     2881 ccagcagttc cgtgttaacc cgcgtcgctt cttcaagcat cggcgcaaac cccgtgccgg
     2941 caatactggt ccccggctgc tgatcagcat ccccgctggt acatgccttt gcatcaccac
     3001 aggtccgcat ggatttatcc cccagaacct tcaccaccgc ccgggcagct tcctctgcat
     3061 tacggtattt catgcaggca ctcccgttac agttcccggg actgacactg gatgtactgg
     3121 tcgaaggcag actgttcatc atattgaaac cggcagctgt cagatcatgt gtcggccgga
     3181 tagcagactg tcccgctcca ccacgctgct gccctccgat ccagcgttgc ccctccaccc
     3241 cggtaacctg acgacctgct tcatcccctt tgatcgcatc accgtccccc gtattcacaa
     3301 tttttttgta ctcctcgagc atggccgcct gcgtccactt attgctgtcc gtgaactcca
     3361 tcatgcgttt cgccatgttc tgacaattga gctgcgcttt gtcgaatgcc acattcgcct
     3421 gcagtacacc gttggtcagc atgtcataca gccccggatt agcccgctgt atcaccattg
     3481 ccggcagact cgccacggct ccgctcgccc cctgaatcac atcccccatc aggtttttga
     3541 aaccatcagt gatcccgttc agctggttac ccactgttgt tttcaggtca aaattgccac
     3601 acatcagatc actgctccac ccgagatcca ccccaagttt ctgcatattg ctgcgcgttg
     3661 ccggctgcga tatcaccgag ccccctccca gggtataaaa cagtttgtct gatactgcgc
     3721 cgtccacgga tttaccgtag ccaaacgcac tgtcattcac tttcggcagg gacaaaaagt
     3781 ttccgtcagc ctgtaccgta cccgcacaaa gcaccacagc cagaacaggt atcgtcattt
     3841 tctgtttcat cacttcccct tacttaaaaa ttcgtactgc ccaggaatgt ctgtccccgt
     3901 ttcttgcagc aactgtaggg ctgccacagg gcaaaggcag cattacctgt ttcagccgct
     3961 tcatgctctc cgtcagggaa gactgcgcag gacgtggaga ctgaaggcgt cagtctctgc
     4021 catttatggt tccgggtgcc cgtattttcc tccaccggat ccggtggcca gtaaccgtcc
     4081 ttcttccctg ctttcaccgg ctgatagaca tgtggctcac cggtgcgggt gataatgtca
     4141 gccacccgct gggccaccac cgcagcagct ttatcatcat ctgtctgggt cacgaacccg
     4201 gagcggggat acacattgcc ccacatattc tcttcttcct ggctgcctat ttcgcgttta
     4261 ccggggatca gggcctccgg gtacagtgac tccggtagtc ctgtacgcca ggcaatactg
     4321 tcaagcgtac tgagaaaata aggaacaaaa ggtgtcaccg gtgactcaca ggaatatccc
     4381 ggcaccatcc ctccgataag cgtggtggcc gggtgccctg ttgcatcggc atatttaaaa
     4441 cggattgacg aacgacgttc cccctgtacc ttcatgtctg cggggttccc tcctcctgct
     4501 gacagaccgg caataccttt gcttatggca ttctccggac caccggctgt actgctgacc
     4561 gccgacattt cacgccaggg atttccgcca ggtgcggtat aggtggaaac aaccacttcc
     4621 gggatataat gggaaacttt cactgatgtt ctgaccgtgc aactgaattt ggtgcagaac
     4681 agccagtagc aaatcccgct gacccgccag ctgatacagc tctgtgaggc tgcactggcc
     4741 actatctgcg cggtgttcag cgatgcctct gccaccggca ataccgccat acttaacagc
     4801 agaacaccgg cgcgatgacg tgatgtgcag gaaatcatgg ctgccctccc cctgtacgca
     4861 gttgttctgc cagcaccacg tctgtggttc cgtagaccac ttcactgtca tcaaaaacca
     4921 cagccggata ttttttcagt ccaagggacc aggcatgtgc taatgcacgg tagtgttcag
     4981 caagccctgc ctgaaacgac tgccattcag gcgactgcat ccgttccatg acacgggcag
     5041 atgcttctgc aggctctgcg ggaagtaccc cgaaaaatgt atcctgcgtc tgctcaggag
     5101 catcaagcag gacaacacga acacctgctg gcgggttcat agggcgctgg tgcgaatcgg
     5161 tataaattgt tgtaccggca gatgcagcca ccggtagtat caggaaggaa tacagtaaca
     5221 gtcggtacat gatgcgactc cgtcagacaa ctggtatctg acagcgtgcg caaaggcaaa
     5281 gggacaatca gcaataaatt caaaaccgga tgataaaaat taagcctctt cctctgaata
     5341 acgcacactc tgcatctgaa tcaaattaat ccggttgcat cacaggaagc cactgattcg
     5401 gggtaatacc ataataacgc ctgaacacag aaataaaata agaagcactg gcatagccac
     5461 acaactctgc cacccgggaa atacggtttt ctcccgcaga caaaagctga gcggcatact
     5521 gcattctctc tttcagaaga agctcactga aggtcgtcag ctcttcctga agctttttct
     5581 tcagtaaact ctcacttata tataacagac tggccacttc acccagcatc catggacggg
     5641 acaaatcagc actgataata ttcttcaccc ggacagtgca gctccggata caccccgtga
     5701 gcagggcgtg gaaatccgga tgttcctcaa acagcgccag actggaaaaa gccaccgcat
     5761 ccctgaagta caccggcgat ggttcatctg taataagttg tctgatcaga cattccgcca
     5821 gcacggacat ctgtggccgg atctgtaaaa gcaaagacga cgggctgact gccataccgg
     5881 ataatggcgt gcagaaccgc agataccgcc tcaccacctc ctcactgagg tcagcgataa
     5941 ggaaacgtcc ggtatgagcc tgaaaagcag ggcgcagatg cccggcaatc agaagcattg
     6001 tgccggcagg tatgacagag ttcctgtgct ccagacaaag cattgtctgt ttgagagcca
     6061 taatgacagc gcaggtacag ggcatcggat atccggtaaa tatgctgatt tattaaagag
     6121 acagacatgc cccgtatata aaattccgtg tcaccgggtg ctgaaaaaaa ctgcaccggc
     6181 ggcgctcttt tactcctccg ttacggatat ttcagccccc ttttcaggag cggtatcctt
     6241 cacatcactg agaccggact gagtcgcttc agagacctct gccacctttc cggctgcctg
     6301 tgtttttact tcctccacgg catccttcac ctcgctgagc ccggactgag ccgcttcaga
     6361 gacctctgcc atctttccgg ctgcctgtgt tttcacttcc tccacggcat ccttcacctc
     6421 gctgagcccg gactgagctg cttcagaaac ctctgccact tttccggcag cctgcatttt
     6481 cacgtcctcc gcggcatcct tcacatcact gagttgagtc tgcgcagctt cctttatatc
     6541 tgccgccttc ttacttacat tctcctttgt cgcatcatca cagcccgcaa ccagaaatgg
     6601 tgcggaaata cataacgcca gaattgtttt tttcagcata caatctctca ttattaagtg
     6661 ttaacgcaga acagcttata tttcgtcccc ggaaaatggt caaaagacaa ccagaacatc
     6721 acagtgccag ggcacactcc cggtatatgc ttttataacc ataacatgct gttcctgata
     6781 ttaccggaaa tgtgcccact acatttaacc tgttttcagc accgggtcat cggcggtgct
     6841 gtattttttt attcccgtca cagaaacaca ctattaccgt tgatttcatg acgaataaaa
     6901 tgaataaatt ctcccgtggt ctttaccacc attattatac caataacacc atttacacag
     6961 atacactccg ggactatctg tttttgcccc ctccgactcc gccggaggtt ttttattttc
     7021 atactcaggc cttcaccact tcacaccgga agtcctccac cctgacgcca atataccgtc
     7081 ccggctcttt tgcgggcgca aacagttttt cacattcagc ctgagtcatt ttttcatcct
     7141 ggtaacgcag ccactgaacc ggcatattgc ctggtttgat tattgtcgca gttattttat
     7201 atcgctttcc catactggct ctcatctcaa caacaaaatc accggctgag ccatcactcc
     7261 ggtaatgcat gcgaaaaaaa gtgagcgtct cagcaatggc tcatgcggtg attctgtacg
     7321 gtacccgcac aaaatacggg caccaacagc gtcgcagaat acaacgaagg catcagggca
     7381 gcacctctct cccgctattt ttcagagttc tttaccctcc tcctgacacg ggagtacaac
     7441 cctgacagca tccgggccag tacattcgca agcacccatg aaaacacagc cacacccaca
     7501 gctcccgcca gaaacagcac aggttcactg gcaatttgat ctgacagcga tggatacagt
     7561 ggtcccggaa aatcgatatc gctcctcata tcacccctct cccagccaca acagcagata
     7621 gaacaaatcc gccgcatcag tcactgatat ataccagcgg ggatgaatat actcctcctc
     7681 cgacggccag ctctcatgcc cactcacagt aagcagaata tgcacatact gaccctgctg
     7741 accttccagc atcaccgcca cactgctgtt cagacccacc ctctgtggct ggcaacggat
     7801 acactccacc cgccccagtt gcgccgccag atgcccgcga agacgttcaa gattatcccg
     7861 tgtggtgcaa cttatccccg gcgcaccctg tggcggcaga acaaacacac ttccggcggg
     7921 gtgaacagca gacaccactt ccggttccgg atacttcaga cacagcatag tctcactccc
     7981 tgccagccag accacgtagc ccgtccagct ttcgtgccac atgaaacgct gcttccagtt
     8041 cgctgcactg gtgcaccttc atcagggcgc gtcgttcagc cttctcttct ttctccgtca
     8101 tccccagagc cagatacaga ctcggtggca caacccggaa caacgcttct gtgcgcttcg
     8161 ccagtaccac cccctccgta tagcagccgg acagtttact ggctgacagc agtaccgcct
     8221 tctgctcctc cgtcagggat ttaaaacggg caatattgtt cacttcctcc ggcggcatgg
     8281 tcaggcacac ccaccactct gccatattca gcatcttctc cgagacatcc ggataatcct
     8341 tcaggttctg cgtggccagc cacagccagg cccccagctt gcgccacatt ttcaccactt
     8401 tggtcatata cggggacaaa agcgggttta ccgtcacgat atgcgcctca tccaccacaa
     8461 acagaatatc ccgtcccaga tactgatcgc gctcggcaat gttgttaatc atattggtca
     8521 ttgacaccat ggtcagtgcc atctgggctt cataaccttc acgggcaaga tgccccagat
     8581 caatcagggt cacatccgct tccggccaca gttttccttc ccggttaaac agctccgctt
     8641 caaaactgcc cggctgggtg aacatcccca gcgactctgc catctcagca gctttagccc
     8701 ggcgaacatc attacgctga gtgtcccgcg aaatttccca caacgcactc tgcagatcca
     8761 ccggcagcat ctgacgcccc tcccggtacg tggtgtgcgt ggccatcagc agtgcctcac
     8821 ggatcatcgc cctgtccgca cgtttgagcg ctgcttcttc tttcggatcc cctccggtga
     8881 tcatcatgcg ggcagatatt tccatctccc ccagaatgtc gcgtttctct tcctcttcat
     8941 ccccctcatc ttcatcaaga tcgggcagtg aatgctcatc cacactctgc agcgtctctc
     9001 cctgctccac cagttgatga gcatccgcaa atggaggaag acacaccccc gttcccggtt
     9061 tgacactgat tttgttaacc gaaagcccga gactctcaaa ataatccgcc agcagaccaa
     9121 acgagttccc ggcctcagcg ataaacagac ggggacgatg aactgccatt atctgcgaca
     9181 acgaaccaca cagcgtggcc gatttaccgg ctccggtagg cccgaaaagc agcagatgtg
     9241 cattctgggt ccggtccagc ttattcagcg gatcaaaggt cagcacatcc ccgccacggt
     9301 taaagaaact gaatccggga tgccccgtac cggtttccct tcccgtcacc ggcaggagcc
     9361 ctgccagatg ctgtacccac atcagacgcg tgtaccactg ttttttatcc gtatcaggat
     9421 caaaacacat gggcagcgcg cgcagccagc tgttcagcgg tccgacatca aactccgggc
     9481 gcactggctg taagcccgcg cccagcagca ccgtcgtcag ttcaacccgt ctgcgcttca
     9541 gactctccat atcctctgcc cgcagcagaa acgtgatccc ggcacggtag agcttgtgcc
     9601 ggtttcccag atatttcttc acggtcgcca catcctcccg ggcgcgcagg gattcactgt
     9661 tctccccgac cgcatttttc gacaacctcg tgaagcgctc ttccagtgtg tcctgtggct
     9721 ggaccacaat tgtcatacac agcaccgttc cctccggaaa ggtgtccatc agggcattca
     9781 ctgtcttttc gccccgggcc tgttcccctg tgatggttcc cggctccggt ggtgtgcgca
     9841 gtttctccac cacaacagcg gcatgcggaa gcccgtccag ccaccacacg ccgttttccg
     9901 gatcagatac cggtggggtg aaccagaggc tttctgcaaa atcggtcatc accggcatca
     9961 ttccctccgg tgtctcctgc ggttctgcac gggatgccat acggtacaga atatccctgt
    10021 ccacccattc cggccgggga ttgaacagcc tcagcagcca gccatgcacc tgctgcccgt
    10081 tcatccgtgt acagcggacg cctgcgccac ccagcgcccc cacgacccgc gaacagacct
    10141 ggttcagcat tgccaccggc ggcatcggat cgtggttgtt tttcccgatc caccggtaaa
    10201 tcaccatcct cgtgcgccgc tgctgaccac gccatggctg acctgtgacc agcgtatccc
    10261 tgaacagacc ttccggtcgg gcaatcccct tcagatggcg ctccatttcc ctcagccagg
    10321 cctccgtaaa tgaggagcct tctgcatgag gtttcacgta ccccctgagc tggtcaacat
    10381 acgtgtccac gtcgttttca tcctgacaga agaactgcac cacccaggga tgtgagtcat
    10441 attcatcaaa actgtcctgc agcgcatcct ccaccgtatc ccggatttgc tccagacgct
    10501 ctgcggtccg cccttccgta gcggccggac tcacctcata cacggcacca accgacaccc
    10561 catcatccag cagcaggcac tgctcttcgt ccagatattc cacccatggc agaaaatcaa
    10621 tcaccgacgg acaggcatgg taaaccttcg tctcatcctg tcgggtcatc ttccccggcc
    10681 ttgacagtgg ctttcgtacc tgaggggcca ctctttcttt catatccgga gataccgctt
    10741 ctccgtcatg ctgcgacacc ggggaagaaa cccgtttttt tgtgtctgta aacagggaaa
    10801 atcgcatcac aacgcctccg ttcgttcacc cggcatggca tactgcgtcc ggctgtaaaa
    10861 aggaaatact gtgctgtacc ccggaaccgg tgacgtaccg gaagccagat gcggaaaaac
    10921 atacatcacc atgtcaggat tgggcagccg cggaaactgc tgactgatct cactttcctg
    10981 tgtccggctg tagcttatgg ctccgtgcgc atctgcctgc tgctccggca ttgtcagcgg
    11041 acgccgcagg gtgtccctgg ccgctgtcat gtgccgcgaa tgtcctccgc ttcctttccc
    11101 ctgccagatc tccagcatac tctgttcacc cggaggcaga agctcctctt tcgatgtcga
    11161 gcagccggac agaataaata ccgccaccat gacggtcatt acagataact gtcgtttcac
    11221 gggatctcct tacatcagtc cagaccaccg gtaccgtgga cttcccccgg cagagtgaag
    11281 tcgtaacgta cccggcgccc ctgatcttca taatcaatgg ccagttgacg cgtgatatgc
    11341 accgccagcg tctgccccgg gggaacataa atggcatcaa acgtctgccc gtatcgctgt
    11401 ttcacccatt ccgcggtttc attcatcccg ccggacaggg cttttccaag tgccgcctct
    11461 ccggcactgc cggtcagtgt tgatgtgatg ccattgacgt tgttctgtga ggtgtactgc
    11521 ccctcactca gtgcatcacc cgcagcgccg gctgcagaca gggcaaatat tgtcggcaga
    11581 tacgtggagg cattggattt acgggttccg ctgatacacg ggatcccgtc atcatccgaa
    11641 atccagccaa tgcctcctgt actgttgtta ttttttgcct gtgcgttact gctgccgttc
    11701 acgtctgatg acggcagcgt acgcactgtc ccgtcggcaa aaacaaacgt gatgctgttc
    11761 acctgcccac ggacacagga gagcgtccag tcaccactcg cagtaccgga caccactgca
    11821 ccttccacat ccggcagctc tattccgttt gccgtcaggt tatccttacc caccaggatc
    11881 ttgaacggat agggatccgt cacggttccg ttcaccggaa cccgtcccag aagcgctgtc
    11941 atcgcccggc tgcctgccag cgtcgagttc tccggcaggg tatacactgc ccgggtgctc
    12001 gccttctgct gttcattgct gtatcccttc acctgctgtt cataagccgc tttctgacgg
    12061 gtcagtggat tgtcatccag aaaagagact ggaaaacgcg gtccgctctt atcccccacc
    12121 gacgacgctt catcctgcac ctgatcggcc ggtgtcaccc acatcagacc atccgttccc
    12181 ggtgaggctg ccgatcctgt cacgctcccc agtccgtcaa ggcccagacc tgctggaata
    12241 tcgctctcct gtcctccggc gccggcatca gcactctcct gctgacgctt cagttgctct
    12301 gtcagtgact gaacctgtgt cattaacgcg ttctgttcat tccggaactg gcgctgcgac
    12361 tgctcataat ttttctgcaa tcctgccact gcctcactga cctgaccgga aacatccgtg
    12421 ccccgcttgc gcagcatttc attttctttc agcaactttt cattctgttt gtccagtacc
    12481 agttgccgct ccctcacctg attcatacgc ccaatcagtg tgcgcagggt atcttcaggt
    12541 gtgtctcctt ccacgcccag ggccttcagt tcatccggtg acagtccggc cagcgttcct
    12601 gagctttccg cagacgaggg ctccccggta tctcctccac agctttttaa tcccgtcagg
    12661 atccccgcca ccaccacaca gggcaccaca tacttcacga tcgtattgga tttaatctgc
    12721 atgcttcacc tccccccatg aaggaatgcg acgggaggca ggctccgcga taaacgcctg
    12781 ttccggccgt tctgccgtca ccacatagag cgtggtggta tcctcagggg tacctgctgc
    12841 acccagccag cggtgctgga aagtcgcggc aatgaatttt ccctgaagtg cccgcggatc
    12901 aaggataatt ttgcggctta ccgtactcgt cagtttcagt gcaacaactg cacgcccacc
    12961 cgcagcccag gctgccagtg gcgtgactgt cagtggctct gacggataga gtgtggtgat
    13021 tttcccgggc agatgtgtat taaccggccg gatacccggt accgcttcca gcgtccgtaa
    13081 cggtgcgtac agcgactgtg ccgcatagcg ggtcaacaga acgggtaaag gctctgcata
    13141 cttcaccggc tgacggaggg cctgcgagcc gtcatccccg ccgggtacca cctttttgtg
    13201 ttctgcagag cgtgcagacg ccgctgtgcc agcctgactg accgtactga cgcttccctc
    13261 atacacaatc cgtacgggct ctgtgggccc tttttctgct gctgtcacat ccagcagaat
    13321 aatttctccg ctctccacat cctgcagttg cagacgcgtc tgcggaaaaa cactctctgc
    13381 tttcagatag actgccccac cggtactctg taccctcaac tttccgttca gtgctggcgg
    13441 gaagcccacc cgcacatttt tatctgcaaa aataacccgc tcctgtcctg ccatcagggt
    13501 aacgggtaag ggaatgcgct cccatttcat cagctcatca gcccgtgcgc tcagtggtaa
    13561 aaccagcagt gacaggacca tcacaatact acggtataaa caacgttcag ctccgttcat
    13621 cactgattat cccccttccc ggctgccggg gaggctgctt ccagccgctg tggtgtactg
    13681 ctgtagcagt ccagcgccag tccgaacgga ttacgttcag gatccccctc catgcgcacc
    13741 acctttatcg gataacggac aagtgcccgt tttaccggct cagcatgaaa atactcatct
    13801 gccaccagat ccagctgtac ctgccagtta tcccgccctt ccaccgtcac cggatttttg
    13861 ttgtacctgc ccccgtagcc atgttccgga atttcataca ccacgcgcac acgctccagc
    13921 agctcccccg catcactgcg acggcgggca tcactctcca gaatctcccg acaggatggc
    13981 gtcaggtagg cggagagcgc atgaattttc gccgggtagt ccacctctcc gttcttcggc
    14041 caggcattca gttgctgaaa gatataaaag gcaaaggaat aaaccgttga cggcgggact
    14101 tcccaccagg gacgggtact gccggaacgc agatccggcg gattgtgaat tgtcagtctg
    14161 tccggtgccg tcatccaccc gatacagacc agcatcagaa ttccggccag cacaccgctg
    14221 aaaatacgca gtgtcaggat atgctggtcg cgatcccgca gagcatgacg aaaacgactc
    14281 attttttccc cctgctacgg gtacggcgca gggaccagcc atgcgcctcc cggataaatg
    14341 ttttccggcc accaccccat gcactgcttt tcttcgccag atgctgccag agccagttgt
    14401 caggttttcc ccgcttcagc cggctgatcc agacgccacc gaaccagacc agcagcaggg
    14461 gagtcagcaa catcccggtc gggataatca cccagccaag tagcggaatc agcggtaacg
    14521 gccagatccc ccctgccaca gcgcctgtca gggccgccag ccccagttcc ggtgtggtaa
    14581 aaccacgaaa aaccgcaggc tccacgttca gacgatcggg caaaaaacga atggtctgca
    14641 tggggatgtc tccttacaga atgatggagg ccgacttgcc cagcagccag ataatggcga
    14701 ccagcagaac aacaccaacc accacgatgg caccaaactg cccccatccg gccttcccgt
    14761 cacgaacctc tgagaaggtg gaaagcgccg cttccgccac cttcacaaaa gcaactgcac
    14821 tgagtacaag accaaacagc acaacaccat cctggagata gcctttgatc tgtccgaaaa
    14881 gtccaccacc tccaccggat gttggcggct ctatctgagg caaatcagcc agagcaggtg
    14941 tgatcccggt acacagaaac accgccatgg cataccaggc ctggcgacag atgcgcattt
    15001 ttttatgcat tatttcctcc tgaaacttaa acaagacact gaaatcaacc cgcaaacatc
    15061 cagatactgc agatcagcag caggacgagt cggaacataa accagccgaa cacaccatcc
    15121 cggacatcac cgctacgcca gcctgcccag gcatccgtca gactccacgc tgcccagagg
    15181 aacacgactg ccagcagaaa tcccagcgtg atgagatgca gcacactgag atccacttca
    15241 cctgaagcag cccggaaggc cgtattctgc agggccgtca tcgccatcag ggacgctccc
    15301 gccggtagtc accgatcacg gctgcggaag accggggttg cgcccgggaa ggggccagat
    15361 aatggtcaat accgtcacga atggtctgca aatctgcacg ggcacgcaga taatcaaaaa
    15421 agtagcgttc cccctgtgaa gaagcctgca gagaggccgt tctggcccgc tccagcgctg
    15481 cctgcacgtg gtcaagctgg cgcattacag tggccagttc atccttttcg gcagcctgcg
    15541 caggtaatgc aaaggacaaa agaaacagca tccccacggc accggccaga cggccggcac
    15601 ggcaggatga tgccggaaaa gaaaacatac gcacctccgt cagagttaaa cagtgcgtac
    15661 aggatgcgta aggcagggga ctggatcagc gataaactct atttgcaaaa cagaaaattt
    15721 ttacgggaga atacactggt gtggatgaag gcaaacaacc cggaactcct ggcatgaacg
    15781 acatcatcag tataatcatt ctcctgacag tctatctcat cggcctgata ttcattatcc
    15841 tgactatcaa tgatatagac agatcatcaa aaaagagaaa gtacgagaaa aaactgaaag
    15901 aagcgataat aaatggcagt ttacaaaata atgatatctg tttactggcc gaacgctggt
    15961 ctgccagtca tgaacatatc aaaagcgcac tgctgtctgt tttcaatgat tttctgactg
    16021 ataacaactt ttcacatcac gtcccccggg tcagagaact gatcacatgg tacgagaaat
    16081 atgccccatt cacagaactc cctgaggata taaaacaaca gttacaaaat ataatcagtt
    16141 tacccgatgt cagtgagtca gaagtgcagt tattagccat gtcacttcat gacatttata
    16201 attcctgtca gaaaaaagag aagctggcca gaagaataaa ctatgttctg ggggctgtca
    16261 gcatagccag tttctgcatc tcgctgttcc agattctgtt caaataaatt tattccagcc
    16321 tgaatgattt attcctgttt attaaaacac ggcatcacta ctgtttcccg ttaatggcac
    16381 cgcattaaca atttaaagag gaaacacatc agcatggaat ttctccgtac tgcccttccg
    16441 ttctgggccc tgttccttct gacctgggtt tgtgttgtgt attttttcat ttatgaagaa
    16501 cctcgttgcc ccccgtggat ctatgacgtc atcagggcca tcactctgtt cattattttc
    16561 gtcaacattg ttgtcattcc ggcgctgtcg ttactctgac actgacgcgc actgaccgta
    16621 agggcgtggc ttgtctgccg acaccctgcc acaaaacaag ttttgcatca gggtgtccgg
    16681 cctctgtgga ttataaatat ttcttgaagg atgccatggt cacggtgatg gccaccccca
    16741 gcagcatggc tgccggccat atcagcaggt tcggatacac cgacgtcggc caggacaggt
    16801 aaagaatgca gggaatatat atcgccggtt taatagactt ttttgcatgg tgataaacaa
    16861 agctggactc atacgcagca ccataccggc gcagatcacg acgccccagt ccctccacaa
    16921 ccgcgatcag tatcaccatc acaaacagcg gaacagacaa caccaggatc gtcacccgta
    16981 tcagtgtgac caccgtgaca tatactgtgg ccatcaggta ctcatacaga taatggctca
    17041 gccagcgccc cacatcctca ctgtactgtg acacactgtt acccaccacg gcaccggcat
    17101 tttgctgctt cagccacgac agaaaacctg tttccacaaa cagccactga tacgccgttg
    17161 ctatccattc tgtcaccgtt accgatggtg ccgacagcag cagactgcgg gtgaattccg
    17221 aagacagcca ccccagttct gtcttcatca ctgcctcact gtgtgctgcc ccctgctccg
    17281 gccagaaaaa agcaatcccg atatactcaa ccagcaggct cagcagcagg gaggaaaaca
    17341 ggatcccgac cagcatccac ggccagcccc acaacagatt ccagagcagt ccgtgctgac
    17401 gtggcggaga gggttgtgct gctctgttca cctcacgacg gggttcagcc attctgctct
    17461 ccttccggca tgttcacgga gggagtctca ttccaccatc cctctccggt gtggtaatta
    17521 cgccgcatgg cctctgcaat ttttgcaata ctctccggca tcagcgcgtc atctgcatca
    17581 gttgcaggca ggggcatacg gattttccac aactgtcccc cttccagcag agcaaaggcc
    17641 tgtcctttcg gcagggtcac gatatctgcc ggctccagca aaggcacctt cacagtacca
    17701 acacgatcct gggtactgga tgtgaagtcc tggccggtcg tgacatctgc ggtgtcctga
    17761 tggccggaaa ccagcgtttt ggtataaatt tccacctggg acaactgggt cgtcagtaac
    17821 tcggccgtcc ggttctccct gacccgcagc ataatcatgt tattaaagtt accctgcacc
    17881 tgggccgttt tggcagcact accggtacgg gcctcaatat cagaagaggt ctgcgtgtaa
    17941 gccgtcacct gcataccggc accgcctccc ttgttgatta acggaatgaa ctcatcaccc
    18001 atcagttcat taaactcatc acaatgcagg ttaaccggat ttttggtacc attccctccc
    18061 ggaagaccgg cattcattcc gtgtttgtag atatgcccgg cgacactcac cagatccgca
    18121 aacatggagt tacccacggc actggccacc tcactgtcac tcagcgcatc aagaccaatg
    18181 tacaccaccg cctttttacg tatcacctgc tcccagtcaa acacgggacg ggtatcgctc
    18241 atatccaggt agtcaggtgc cagcagttct gccgtctttc ctgtggtcag tttttccagc
    18301 aacggcaaca gggatgccac aattttgtca aaataggtac gatcataacg taccgcgctt
    18361 cgcagaccat ccagtatcgg atcatagagt ttctttccgg cttcactgct gagagccacc
    18421 tcaatggccc agatacgcac agcatccggc tggttctgca tggtccgggg aacatccgct
    18481 tcccccagta cattcatgtt attcagaatg gcgttttcca gttccggcat ctgttcgctg
    18541 atcaccttac cagcataacg gatataaagg tcggcaatat tattcacata acgcataatc
    18601 agcgtgtaat ccgggcgctc ccccagtgcc accagtgcac gggcaacaat gttgacaaac
    18661 cgccatgcaa actcacggaa ggccgcactg ttaccttcac cggataactg accggccaca
    18721 cgggaagcca cctccgacac acgaccaaaa cgccccacgg cgttataacg cgcagagatt
    18781 tccggccagc ccagatgaaa gatataaagc tcatcgcccc tgcctgcacg gtgtgcttca
    18841 gcccagacac gcagcaacag atccgcatcc cctttgggat caaagacaat ggtgacatct
    18901 ccccgacgaa tatcctgtgt gaccagcaac tccgccagcc gggttttccc gacacgggtg
    18961 gtgccataga caatggtgtg ccccacacgt tcacccagtg ccagcgtcac atcctgctca
    19021 tccggctcaa taccatgcag ggcaggattt cctcccaccg gcggcagggg acggacggga
    19081 ttcagcggcg agtcacgacg aagaagctgc ccaatccagg gaagactgcg ctccgttttc
    19141 atttcaagcc agcgcgctgt ccggtacaga ctgcccggct gaagatagtg cgcaacctcc
    19201 ggtcgcagcg tgtcctgaag gcgctgggtg tgcttctgtg tccagcggaa gccgcgtccc
    19261 agaaacagcc gctgacggct gaccggaatt tgccgggaac tcatcacata acggggcagc
    19321 cggcgcagat tgcgacggta acggatgatc ttcatcccct gtcggaaacg gaccactgcc
    19381 agaacgccaa agccggcggc tgtgacatag ctgaccgaag gcgcgagcgc caccgcccag
    19441 ggcgctgtca cacagacacc ggccgccgtt gctgccacca cggcggtatt cagttccaca
    19501 gccggacgca gcagagcctc aatcacataa cggttactca tgaccgggta cctctctgta
    19561 aaagacgccg ccaccatgag cgcggcaacg ctttctccgg tgacgctgac agtgcctcca
    19621 gcctgcgctg taacaccatc acctcccgcc atgcgtctgc cgcattcagc agtaatgcct
    19681 tatacaccag tggcccgtca tgtccgccgg catgcacatc atccactgtc aactgcgcct
    19741 ctgttctgaa acgatccact ttccagcagg ccaggtcttc gcgggaaaca cactgccgcc
    19801 ggatctgcag acgaagatac acatcatgcc gggacaacgg ttctgccagg ttgtgaattt
    19861 cccgacaggc agaccggaac gcctggtctg tgtgataaat actctgggcc acattgtgaa
    19921 gctgtgtgcg cccctccccc tgcaggtgga acagaaagcg ggccagacag ctgcggtgat
    19981 taccgtcagc actgacatac cagtgaatgc catccgtgct gacaaaaccc atcccggcat
    20041 gctgttgctc tgcctgcgtg taatatcccg gattcttttc aagtaacggg atattgatgt
    20101 ccatgcgttc accacgctcc agcagctcca gccagctcat tccggcatac tgtggatggt
    20161 cagtccccac aatgcggtac acattgatac tgccctgtcc cgtccagtag tgttccgtaa
    20221 taaaatccga cattgccgca aactctgcgg tatcccatgc aaccagttgt tcatgtgccc
    20281 aggccggagc attcgcctgt atccgctctg taaatgcccc cttcattcag ctcttctctc
    20341 ctgcactttt gagataccga taccgtcagg ccacggcata acagccgggc cccccctgcc
    20401 ttccccggat acggctgcag aaccacaacc acgaaacaga atgactgacg agacaccttc
    20461 cataaacaaa gcgcccagca acagaaccac tgcctgcatt ttcagttcac gaatatcttc
    20521 agcaacagcc tccccgaaag aaatcgctgt tcgcggacat tcctgctcat cgacatacat
    20581 acctgagacg gatatgacgg aggaacattt ttgtaccatc acatgccctt caggcgcatc
    20641 acgccagcgg gatgactccg tcgcataacg ctgtgtcatg cctgcctcac caccactcag
    20701 attgttccag acatacagca ttaccagaca tcctgccagc attgacggcc agacaatcag
    20761 aagccggagc agcagcataa caaaccggta cagtgtcata atcatgatct tcctttatta
    20821 ctcagtaagg tgccattcag cagttgcaac agacgttgtc cactgatcag ctcaatatcc
    20881 ggccacagtg tcagccgtgc ccggctgatg ttccccgtac gccccgtatg gataaaaaag
    20941 cccctgcatc ccttccggcg cagcagttcg ccaaaagcat caatatgtgc cggacgaatg
    21001 gttcttccgt aacgtttagc ctgtatcagc cagcgttcac cgttaatcac cacctgacca
    21061 tcaagaccac catcgccgct gtatgagggg ttacggataa ccggcatccc ctgttgctca
    21121 aaagccagca gcaacagttc ctcaaacaca tacggactga ttttgcgcag atatttcagc
    21181 cgccgggcat cgccctgcaa ttcgttcagc cgcttcatta cccgtgaggc cgtggcacga
    21241 taacgctgat gccggcgtgt tctcacacac gctttttttc gccggctgcg ccacagtgcc
    21301 ggccccagga caacagccag caggctgaga accaggcagg ctgtcagtac cggagaagca
    21361 gagagcgcag cagaagtggc agacagcggc atcatggtgc tggtacctgt gacaggccgt
    21421 tgtccgttat cagcaccggg taatgactta tctgtaacct gcgggccagt acagaaccgg
    21481 atgccggaac catcaccacc cccggtgcca gctcacgcag tacgcgcaga ctgctctcat
    21541 ccgccacatt cactaccatt cccgccgcct tccgctccct gagtactgct gcattcatct
    21601 gcagccatgt gcgggaatgg gcatcatcgc ccacaagaaa aagtgcaccg atgcccggta
    21661 actgaagccg gcgcgccaca acctctccgg gtgacatttc aggcgtactg accggtaaca
    21721 ttgccgcctc tccctgcgga aaagagggtg atggcacaac tgaagcggga accggtggct
    21781 gccggtttat cgcctcaaaa aaggggctgg catccctgcc gcccaggtct gcaatcactt
    21841 tcagttctgc gccagctgtt gtacagaaca gcagcagact cagtacataa aacagtcttt
    21901 ctcgtttcat ttcagctcct cctgtggttc agtccagata taacgggggg atggtgtcag
    21961 catggcggtc gctgccacat gcacatcaga cggattcagg aaattatccc ggtggagccg
    22021 ggcaagatgc cgtctgacca cggcggtata acgtgcggcc ggcgcaccac ctgccgggtg
    22081 gtgataacac ccggccacct gcagccagct gaccggatgg cgacgccggc attcctgcag
    22141 aatgaccgct gccgtattca gactggtgta aggatcaaat gccgcccatg tggacgggaa
    22201 acgatgtcca ttccagccca gattgacctg tgctatcccg acatcaatac gttttgccgg
    22261 ataatgtttc agaaacacct gcaatgcctg ccaggcctcc agacgtgtgg cataacggta
    22321 cccttttcct gccacattta ttgtccaggg ccaggggcgg gccacgtccg gctgacgaaa
    22381 atcacgacgc cgttgtttca gtactccgga catcgcttcc ggagacatgg acgtttccgt
    22441 cagggaaacg gcatacaaaa gctctgcggg aataccatgg gcacgggcca cccgcacgta
    22501 cccgtccggt acgttctgaa cggcagcacg acagacacca ctgcaggcca ccatcagcag
    22561 aaccgtcaga atgccgccac gcgccatccg tcagccccct gctgcagtac caccggcatt
    22621 tttccttcac caaaacgcag ccagcgtccg gcatcatgat tcagggtgat atgacggctg
    22681 cgaactttct ccacaggtat gctgtgcgca tgcgcccact gacgcagaat ctcatccttt
    22741 ccctgactgt cgacaaggta gatatcaacc tgccgtcccg tggccagtac ggaggcaaga
    22801 cgggcatcac aggtgccaca ttccttcgcc ttcacaaaca gcgccagccg tccccccgta
    22861 tctccggtaa gtgctcctgt ttttcccata ctgacaggca acataccggg atacaggcgc
    22921 ttccaggccg cattcacctc ctgctgaaac gccagctcct tctctgttcg ttcatattca
    22981 gccctgaccc acttttcggc ccagacacgt cgctctgccg gcgttctggc ctctataccg
    23041 agtaccgtca gcggatcaag tccgggagac tgtatccccc gggggccgga catcagtgac
    23101 tgataacgcc ggtaatcctc ctcactcacc ccccactgcc cggcctgctg ctgaagtccg
    23161 gatgtggtac tctgcgtcac caccgtaccg gtcgtccgtt gcgcctctga cgacatattc
    23221 gtctgcccgg cggactgagc cagaacgggg cacagtgcca gcagatacag tatttttcgc
    23281 ttcatcatct actccaccct gagcaccgtc tgacgaccat tcacccggaa cgttgcttca
    23341 ccatggccgg catacacaag cgtccacccg gccacactct ctccttcacc cagcagagcc
    23401 acctgagaga ggctggtata cccccgggga gccacggctg cacgggtact gccaccgcgc
    23461 agctctgtcc cggtcagcac aaaaggagcc tgccgaactg tatgaactgc gcgggattta
    23521 cgggaggacg tcaccggagc gggctttttc tcctccgttt ttttcatccc cgctttcctg
    23581 ctgtccggct gtgacggtga ctccggtact ttctgcattt tctccaccag accgctgagt
    23641 gtatccagtc tcagggaaag cttttcccgg gcagactcaa tctgctccag catctccggc
    23701 agattctcca gttgttcctt ccaggatgct gtcagcacgg gctgctgctc caggactgcc
    23761 aggcgctttt ccatatcctt cagttgcttc tccgtctctg tcagtttcat tctcaggggc
    23821 gcgagttctg acgcagaaag catttctctt ctctggcctt ccagggtcgt gatccggctg
    23881 tcaagaccgg tcataagcat cagtacgcca tataccactg cccccatgac cagccccaca
    23941 gtgacagaca gtatcaacac gccggggaaa cgactttttc ttcgctccgg cgacatacca
    24001 gacgcaccct ccggtatctg cggctgtttc tcactcattt tttcagaaac cctcctgttg
    24061 ccttcacacc cggttcacga cccacaaaag gaacggtgac cggaacagga gccagctggc
    24121 tgaccggtaa ctggtaaccg tcacgcagac tgtggcagac caccctctgc acatcatcca
    24181 cttccagttg ccatgccggc cccgccatta actgcagggc agtacgcagt ctcagtggtc
    24241 ctggctgata ctgcaccgcc ggcagaggct gacggtacag cacgccgttg gccggtccgg
    24301 tggcgcagag cgtgtaaccg gactgtttca gggcataacg catggcatcg gccaccgtgg
    24361 gtttcaggga ggagggaatg cgaatgtcga tcacctgaga aagtggatca cgctgtacag
    24421 cctgcggctc tgtgctgacc agcaggtaac ggtcatagcg caccacctcc ggtgcctgtg
    24481 aatacacatc cgggctggca ggctgtacat tacggtttac tgtgaccgac ggtaccggca
    24541 tggccgcatc ccgttgctgt agtttctgag tgccctgaca tcccgccatc aggaggcaga
    24601 ctgtcagtcc ggacaggata aagcgttttt tcatcgggta tttacctctg cagtgataaa
    24661 tcagaggtca atgtgcagaa gaagacgggg ctggctcaat gaacaactca ttacccgctg
    24721 gcgaaaaaaa acgcctgcag acatgcaggc gctttgttga caggaaggaa agcaacataa
    24781 caaatatgtc acaatcctgc ggttaacata aattaccgat taccggagag cattacgcaa
    24841 atcatcatcc gagaactgca gtatttcttt gattttctct gacgatatac ccatctgctg
    24901 catgcgacgg gccgatgcgt aattctcctg aagagccttt tctctctcct cctggcggcc
    24961 cttttcgata ccagccagtt ccagtcgttc agcaatagtc attagatcat cctcatgtct
    25021 tggcagacgt tctgccatcg cccgtataaa ttcaggtgaa gctgaatcac attctttcac
    25081 catatagtta aacaatgtca ccacctgagt gtgtgtttcc tgtatctgaa tcaactgtga
    25141 caaaaagtca agatgaacca tcagatccac tttcttctgc cggagcttca tcataagcat
    25201 cagcgcagcc atctgcttat agcgacggat accctcatca tcaagaagac cgacatcaat
    25261 caccctgggt ggcctgcctt ctccataaag ctcccgacca aacgccggat catccagaca
    25321 gtcaggccag ttcatgctgt atggataagg actgggtaca ccatggtaaa aaagcaccgg
    25381 tatcacaacg ggagcacacg catgtgcttt caggtggcga tgtatcaccg ccagaacata
    25441 gcgcatcatt cgcagggcca tatacttatc cgccttgctc tggtgctctg tcagcgtata
    25501 aatgtatcca tccctgccat ctgtggtttt catactccac agaacatcac aggcaaactg
    25561 gcgaagatcc ggatccacaa acgtggtcgg ctccagttgc agggtgttca tatcgcaccg
    25621 gttcagttgt tccgggggca acgcgccttt caggaaatcc ctcgcaacag agggacattc
    25681 catcatcgct ctgaacgccg catcatgtgg cgtgggggta gtgggtgtgt tttcattcac
    25741 aaccgcctcc ttgaattaaa agtggggcgg taagccccgt ggttaacatc agatgacatg
    25801 gataatccgt cgttcaacgc catacccacg ccccataaag gggatatcat cgtcaaagtc
    25861 catcggcggc attcccccgc ttcctgagga agcctgaccg ttttttcccg ttgattgttg
    25921 tgcattttgt ggtggttgtc cccatcctcc ctgagcagga gcagatgaaa catttccacc
    25981 cccctgctgc tgacgcggag accccagcat ctgcagggta ccattgacac tcacaacaac
    26041 ttccgtggaa taacgatcaa caccattctg ctgatcgaac cacttccggg ttcttaactg
    26101 accttcaata tacacctgtg aacctttacg aaggtattca ccggcaatct cagccagttt
    26161 tccgtaaatc acaacacggt gccattcagt gtgttccttc atttcaccgc tctgtttatc
    26221 acgccaggta tcagtggtgg ccagcgtcag atttgccact gcacctccgc cgttggggaa
    26281 ataacggatt tccggatcct gtcccagatg accaatcagt atgactttat tcactccacg
    26341 cgatgccata acaactcctt atcctcacac acattcacgc tgagtccaga tgccgttttc
    26401 cagagcccgg actgcctgct cataatttct gaaatactct tcggattccc gggacacagg
    26461 cccttcgtcg cactgagtac caatataata accacgagca ctgctgagga tctgcagagg
    26521 taaggtatag cccgctttgg cggccagaat tccgcttgtc ggtttcataa catgtttcct
    26581 taaagttaaa cgataagaaa acagacctcc ctgatggttg gtctgttttc atcagggagg
    26641 ttgagttgaa aaatacgatc agaaagaatt ttctgcgtat tcccgcgggg tattgccatt
    26701 ctgtggcggt atggattccg atttttcagt cttataaacc atgtcctggc ctattttgat
    26761 ccactccacc ttgatcaggc gggctttcag actgacgcgt tgctcaccgg catgttcacc
    26821 ccggttaagg gtaaaaatgt cagtagaggg gttgctcatg gtaaaaccaa tcagcacctt
    26881 gcgatcttca tccaccgctt tctggcagcg ggcaatcagg gaagttgcct ctttaccggc
    26941 aaccgtcaca tcaaaacgca cataggacgg gctgtccgtc ggaccactca gtgcattcac
    27001 aacacaactg agaaatgtcc cgttctgatg attaacctga cgaatgttgc tgagataccc
    27061 caggcctttc agtgtcagat tgaagtactc agttttagcg tttgcagaag aagtatttgc
    27121 agtcataatg atatctccat tctcagtatc ggaaagacag agaacatcat catccctgac
    27181 gggttgtgat attcccgtca gggagccgga acagaactat tccggctgac tcagtaacag
    27241 ttaaag
//
LOCUS       DAZGAP010000056        27204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGAP010000056 DAZGAP010000000
VERSION     DAZGAP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.056"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..572
                     /gene="slyB"
                     /locus_tag="ACHLAM_001975"
     CDS             105..572
                     /gene="slyB"
                     /locus_tag="ACHLAM_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO7853504.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(619..1053)
                     /gene="slyA"
                     /locus_tag="ACHLAM_001976"
     CDS             complement(619..1053)
                     /gene="slyA"
                     /locus_tag="ACHLAM_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO7853505.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            1254..1490
                     /locus_tag="ACHLAM_001977"
     CDS             1254..1490
                     /locus_tag="ACHLAM_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO7853506.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLSLAILIAW"
     gene            1493..2350
                     /locus_tag="ACHLAM_001978"
     CDS             1493..2350
                     /locus_tag="ACHLAM_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO7853507.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSNLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            2350..4362
                     /locus_tag="ACHLAM_001979"
     CDS             2350..4362
                     /locus_tag="ACHLAM_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7853508.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELQHL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGSNALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(4363..4884)
                     /gene="sodC"
                     /locus_tag="ACHLAM_001980"
     CDS             complement(4363..4884)
                     /gene="sodC"
                     /locus_tag="ACHLAM_001980"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO7853509.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4965..5861)
                     /locus_tag="ACHLAM_001981"
     CDS             complement(4965..5861)
                     /locus_tag="ACHLAM_001981"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO7853510.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDYLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5910..6149)
                     /locus_tag="ACHLAM_001982"
     CDS             complement(5910..6149)
                     /locus_tag="ACHLAM_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO7853511.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6252..6851
                     /gene="nemR"
                     /locus_tag="ACHLAM_001983"
     CDS             6252..6851
                     /gene="nemR"
                     /locus_tag="ACHLAM_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO7853512.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATPAV"
     gene            6888..7985
                     /gene="nemA"
                     /locus_tag="ACHLAM_001984"
     CDS             6888..7985
                     /gene="nemA"
                     /locus_tag="ACHLAM_001984"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO7853513.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            8066..8473
                     /gene="gloA"
                     /locus_tag="ACHLAM_001985"
     CDS             8066..8473
                     /gene="gloA"
                     /locus_tag="ACHLAM_001985"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO7853514.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            8576..9223
                     /gene="rnt"
                     /locus_tag="ACHLAM_001986"
     CDS             8576..9223
                     /gene="rnt"
                     /locus_tag="ACHLAM_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO7853515.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            9316..13932
                     /locus_tag="ACHLAM_001987"
     CDS             9316..13932
                     /locus_tag="ACHLAM_001987"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO7853516.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQADEWYSRVRRAAPWKDLPRRVFDVTLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARTLLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANSSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(13983..14330)
                     /gene="grxD"
                     /locus_tag="ACHLAM_001988"
     CDS             complement(13983..14330)
                     /gene="grxD"
                     /locus_tag="ACHLAM_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO7853517.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            14665..15492
                     /gene="mepH"
                     /locus_tag="ACHLAM_001989"
     CDS             14665..15492
                     /gene="mepH"
                     /locus_tag="ACHLAM_001989"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO7853518.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15620..16201
                     /gene="sodB"
                     /locus_tag="ACHLAM_001990"
     CDS             15620..16201
                     /gene="sodB"
                     /locus_tag="ACHLAM_001990"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO7853519.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(16347..17516)
                     /locus_tag="ACHLAM_001991"
     CDS             complement(16347..17516)
                     /locus_tag="ACHLAM_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7853520.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(17682..17771)
                     /gene="cydH"
                     /locus_tag="ACHLAM_001992"
                     /gene_synonym="ynhF"
     CDS             complement(17682..17771)
                     /gene="cydH"
                     /locus_tag="ACHLAM_001992"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO7853521.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVVVGLTAALH"
     gene            18070..19095
                     /gene="purR"
                     /locus_tag="ACHLAM_001993"
     CDS             18070..19095
                     /gene="purR"
                     /locus_tag="ACHLAM_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO7853522.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(19092..20024)
                     /gene="punR"
                     /locus_tag="ACHLAM_001994"
     CDS             complement(19092..20024)
                     /gene="punR"
                     /locus_tag="ACHLAM_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO7853523.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            20137..21348
                     /gene="punC"
                     /locus_tag="ACHLAM_001995"
     CDS             20137..21348
                     /gene="punC"
                     /locus_tag="ACHLAM_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO7853524.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            21639..22787
                     /gene="cfa"
                     /locus_tag="ACHLAM_001996"
     CDS             21639..22787
                     /gene="cfa"
                     /locus_tag="ACHLAM_001996"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO7853525.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(22827..23468)
                     /gene="ribE"
                     /locus_tag="ACHLAM_001997"
     CDS             complement(22827..23468)
                     /gene="ribE"
                     /locus_tag="ACHLAM_001997"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO7853526.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            23683..25056
                     /gene="mdtK"
                     /locus_tag="ACHLAM_001998"
     CDS             23683..25056
                     /gene="mdtK"
                     /locus_tag="ACHLAM_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO7853527.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            complement(25097..26353)
                     /locus_tag="ACHLAM_001999"
     CDS             complement(25097..26353)
                     /locus_tag="ACHLAM_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO7853528.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGSINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            26662..26738
                     /locus_tag="ACHLAM_002000"
     tRNA            26662..26738
                     /locus_tag="ACHLAM_002000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26696..26698,aa:Val,seq:gac)
     gene            26743..26819
                     /locus_tag="ACHLAM_002001"
     tRNA            26743..26819
                     /locus_tag="ACHLAM_002001"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26777..26779,aa:Val,seq:gac)
     gene            26927..>27204
                     /locus_tag="ACHLAM_002002"
     CDS             26927..>27204
                     /locus_tag="ACHLAM_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO7853529.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTNEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPL"
BASE COUNT         6391 a         7145 c         6854 g         6814 t
ORIGIN      
        1 tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac tttgaccatg
       61 agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt aaacgcgtat
      121 tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac accctgtcag
      181 gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat ggcaccatcg
      241 ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc ggcgcaattg
      301 gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc gggcgttctc
      361 tggctactgc tgcaggcgct gttgcaggtg gcgtagccgg tcagggcgta cagagtgcaa
      421 tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat accatcatgg
      481 tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta ctggccagca
      541 atggcagtca ggtgaccgtt tccccgcgct aaataagttt gcgtgtggtc aggttactga
      601 ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat gctcaagttt
      661 tgcgatgagc ttaatcagtt gttccagttc ctctgcggag atgccatgta aaatttccgc
      721 gcgggttttg ttaataacag cttccatttc gctgatcagc ggctctgcct tttccgtcag
      781 tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta accctttttc
      841 ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg cttttgccag
      901 ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa cccaatgggt
      961 ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata tgcgcaccaa
     1021 ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat tagcttgcta
     1081 agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac aaatgtattg
     1141 ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt ttcgcactta
     1201 aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac tttgtgaagt
     1261 ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg tccgtctact
     1321 ttcctccgtt tttcaaagcc ttcgcgtttg gattcgtcat ctggcttgtc gtacaccgcc
     1381 tgcttcgtgg ctggatctac gccggtgaca tctggcatcc cttgttaatg gatttatcgc
     1441 tgtttgcgat ttgcgtttgc ctttccctgg caatactgat tgcgtggtaa ctatgtcaat
     1501 taaaacaatt aagtatttct caacaatcat tgtagcggta gttgcggttc ttgccggatg
     1561 gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa tacgcgcaga
     1621 acaggtttct atcacccctc aggtgtcggg acgtattgtt gagctgaata taaaagacaa
     1681 ccaactggtt aacgcggggg atcttctgct cacgatcgat aaaacgccct ttcagatcgc
     1741 cgagctgaac gcacaggctc agttggcgaa agcgcaatct gacctcgcaa aagccaataa
     1801 cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tctgccgaag aactcgatac
     1861 cgctaacctc aatgttaaag cgatgcaggc cagtgttgat gccgcacagg cgacgctgaa
     1921 acaggcacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg gatgggtgac
     1981 taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt ttgcccttgt
     2041 cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc gccatatccg
     2101 cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac agggtcacgt
     2161 ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa accttattcc
     2221 ggatgtgaaa cctaacgtcc cctgggttcg cctcgcccaa cgagttcccg tccgctttgc
     2281 gctggataaa gtcccaggcg atgtcacgct ggtgtcgggc accacctgta gtattgccgt
     2341 aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt cagggccacg
     2401 ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc gctgacggtt
     2461 gcctattatt taaatctgga tgaaccctat tgggcgatga cctcggctgc cgtggttagc
     2521 tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatcgctgg cagtttgctc
     2581 ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg gttttttctt
     2641 ttgagcatgg cggcgtggct tggcttttgt acctgggcct gtgcgcactt cacgaataat
     2701 gtcgcgtatg catttcaact ggcgggctac acagctgcca tcatcgcctt tccgatggtt
     2761 aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg cgaggtgatt
     2821 gtcggtattt tgtgcggcgg catgatgatg atgatcctgc cgagcagttc cgatgctaca
     2881 gcccttttaa ccgcattgaa aaacatgcac gcccgattac tggaacatgc cagtttactc
     2941 tggcagcctg aaacaaccga tgccattcgt gcagcacatg agggggtgat tggacagata
     3001 ctgaccatga atttgctccg tatccaggct ttctggagcc actatcgttt tcgccagcaa
     3061 aacgcacgcc ttaatgcgct gctccaccag caattacgta tgaccagtgt catctccagc
     3121 ctgcgacgta tgttgctcaa ctggccctca ccgccatgtg ccacacgaga aattctcgaa
     3181 cagctgctga cggcgctcgc cagttcgcaa acagatgttt acaccgtcgc acgtattatc
     3241 gccccgctac gcccgaccaa cgtcgccgac tatcggcacg tcgccttctg gcagcgacta
     3301 cgttattttt gccgccttta tctgcaaagt agtcaggaat tacaacatct gcaaagcggt
     3361 gtggatgatc ataccagact cccacggaca tccggcctgg ctcgtcatac cgataacgcc
     3421 gaagctatgt ggagcggact gcgtacattt tgtacgttga tgatgattgg cgcatggagt
     3481 attgcttcgc aatgggatgc cggttccaat gcattaacgc tggcagcaat tagctgcgta
     3541 ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg cacgctggtg
     3601 ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtgcagat tagcgatctg
     3661 tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct taaattgcag
     3721 atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc gtttatcgct
     3781 gtcactaatc ccccggtgta tgattttgct gattttctta acgataatct ggcaaaaatc
     3841 gttggcgtcg cgttggcgtg gttagcgttc gccattctgc gtccaggatc ggatgctcgc
     3901 aaaagccgcc gccatatacg cgcgctgcgc cgggattttg tcgatcaact aagccgccat
     3961 ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag tcagttgagt
     4021 aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt gctgctgaac
     4081 tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc gttatcgcga
     4141 gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg cgttcagcaa
     4201 aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct tgcccgccat
     4261 catcaacctg ccgcccgcga gctggcggca attgtctggc ggctgtactg ctcgctttcg
     4321 caacttgagc aagcaccgcc acaaggtacg ctggcctctt aattacttaa ttacaccaca
     4381 ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat tatcgccgcc
     4441 aacgtggacc atcagtgctt tgtctttgac ttcatccagt gatttcagac gaggtgcgat
     4501 gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat cgcctaaatg
     4561 cccggcacct tctggccctt catgtttacc ggtattttgt ggatcaagat gcccgcctgc
     4621 ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc ctttggcatg
     4681 aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggcgaaa actccagacc
     4741 tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt gcgacgtgac
     4801 gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa ccagcgccag
     4861 aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct gaataagtgt
     4921 aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg tacgtcgtaa
     4981 cccaatgccg ctttacggat acggaaccat tgttgacggg tcattttcag tgtttcagct
     5041 tcgacagctg cccgtacacg ctcaatttta cctgagccga taattggcag cggctgcgac
     5101 ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt taactcctcc
     5161 gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa cagacgacca
     5221 ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgcagttg gtcgagcgtg
     5281 ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt ggcaagggta
     5341 aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga tacgccaaaa
     5401 tggcgcactt tgccgctctg atgcagatgt ttaaacgcgt ccgccacttc atcggcatcc
     5461 attaacgggt ctggtcggtg gattaacagc agatccagat aatcggtcgc aagattaatt
     5521 agcgactgtt cggcgctctt aatgatgtga tcgcggtcag taatgtaatg accaatgacg
     5581 ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat ccgttcacga
     5641 aggtgaggag ccagtttcag cgcctcgcca aacgccgctt cgcactgata gccaccataa
     5701 atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat aaaactgacc
     5761 agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac aaaacgggaa
     5821 aactccgggc cttgcggcgc aatagtaata cgctgaacca taatcgcttc ctcttatcag
     5881 atatgagagg agtatacgca agattaggct caaaagagtg atggttgctc cggttcgtct
     5941 gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca aaggcgcagc
     6001 acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc ccgactacga
     6061 aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca cgggctctga
     6121 acgggaaaga actctaattg ctccgccact tcgccctcct caaataagat tattaccatt
     6181 attgaagctg ttaatgccca aagtagcaac tttgcttgca ctagaccgac tggtctacta
     6241 cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc ctggcgacgg
     6301 gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa ttactaaaaa
     6361 ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa gcgtttggcg
     6421 ttgccatgct tgagcgccat tacgccacat atcaccaacg actgactgag ttgctgcaat
     6481 ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca ctgaaccagt
     6541 tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc gaagtgtgcg
     6601 atctgtcaga agatatgcgt agcgcgatgg ataaaggcgc tcgcggcgtg atcgccctgc
     6661 tctcgcaggc gctggaaaat ggccgtgata gccattgttt aaccttttgt ggcgaaccgc
     6721 tgcaacaggc acaagtgctt tacgcactat ggctgggtgc gaatctgcag gccaaaattt
     6781 cgcgcagttt cgagccactg gaaaacgcgc tggcacatgt aaaaaccatt attgcgacgc
     6841 ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg tcatctgaaa
     6901 aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt tttatggcac
     6961 cgctgacgcg tctgcgcagt attgaaccgg gtgacatccc taccccgttg atggcggaat
     7021 actatcgcca acgtgccagt gccggtttga ttattagcga agccacgcaa atttctgccc
     7081 aggcaaaagg gtatgctggt gcacctggca tccatagtcc ggaacaaatt gccgcatgga
     7141 aaaaaattac cgctggcgtt catgctgaaa atggtcatat ggccgtacag ctgtggcaca
     7201 ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta gcgccttccg
     7261 cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg atccgtgttg
     7321 aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc aatgatttcc
     7381 gtcaggccat tgctaacgcg cgtgaagccg gttttgatct ggtagagctc cactctgctc
     7441 atggttattt gctgcatcag ttcctttctc cttcttcaaa ccatcgtacc gatcagtacg
     7501 gcggcagcgt ggaaaatcgc gcacgtctgg tgctggaagt ggtcgatgcc gggattgaag
     7561 aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc cagaacacag
     7621 ataacggccc gaatgaagaa gccgatgcac tgtatctgat agaacaactg ggtaaacgcg
     7681 gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg tatactgatg
     7741 cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca ggtgcataca
     7801 cggtagaaaa agccgaaacg ctgatcggca aagggttaat tgatgcggtg gcatttggtc
     7861 gtgactggat tgcgaacccg gatctggtcg cccgcttgca acgcaaagct gagcttaacc
     7921 cacagcgtgc cgaaagtttt tacggtggcg gcgcggaagg ctacactgat tacccgacgc
     7981 tgtaatccaa cattgcgagc ggcgcaaagc cgccgctata ctaaaacaac attttgaatc
     8041 tgttagccat tttgaggata taaagatgcg tcttcttcat accatgctgc gcgttggcga
     8101 tctgcaacgc tccatcgatt tttataccaa agtactgggc atgaaactgc tgcgtaccag
     8161 cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg aaaccgaaga
     8221 agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg gcactgctta
     8281 tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa tccgtcagaa
     8341 cggcggtaac gtcacccgtg aagcgggtcc ggtaaaaggc ggcactacgg ttatcgcgtt
     8401 tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg ccggtcgcgg
     8461 tctgggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt tactgcatcg
     8521 acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga atcagatgtc
     8581 cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc ctgttgtgat
     8641 cgatgttgaa acagctggat ttaacgccaa aaccgatgcg ctgcttgaga ttgccgccat
     8701 caccctgaaa atggatgaac aaggctggct gatgccggac accacattac atttccacgt
     8761 cgaaccattt gtcggcgcaa atttgcagcc agaggccctc gccttcaacg gcattgaccc
     8821 gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa tttttaaagt
     8881 tgtgcgtaaa ggtattaaag cgagcggttg taaccgcgcc attatggtgg cgcacaacgc
     8941 caatttcgat cacagcttta tgatggccgc cgcagaacgc gcctcactga aacgtaaccc
     9001 gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac tcggacaaac
     9061 cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc aggcgcactc
     9121 cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca accgctggaa
     9181 acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc gatgcctgat
     9241 gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcatgc atgatattca
     9301 cggggaatag cgttaatggc agataatcta aacccttcat cgcccctgcc ggacgtgttt
     9361 tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc tgtccagtcg
     9421 caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc gactggctcc
     9481 gggaaaacgc tggcagcatt tctctacgcc ctcgatcagc tcttccgcga aggcggcgaa
     9541 gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc accgatcaaa
     9601 gctctgggca ccgacgttca gcgcaacttg cagatcccgt tacagggtat tgccgatgaa
     9661 cggcggcggc gcggcgaaac ggaagtcaat cttcgcgtgg ggatccgtac tggcgatacg
     9721 cctgcacagg aacgcagcaa actcacccgt aatccgccgg atattctgat caccacaccc
     9781 gaatcactct atctgatgct gacctcccgc gcgcgcgaaa cgctacgcgg cgtcgaaacg
     9841 gtaattattg atgaagtcca cgcggtagcg ggcagtaaac gtggtgcgca tctggcgtta
     9901 agtatggagc ggctcgatac gctgctccac acctcagcac agcgaattgg cctttctgcc
     9961 actgtgcgtt cagccagcga tgtggcagca tttcttggtg gcgatcgccc ggttacggta
    10021 gtcaacccgc ccgcaatgcg ccatccgcag atacggattg tcgtaccggt cgccaatatg
    10081 gatgatgtct catcggtcgc cagcggcacc ggcgaagaca gccatgccgg ccgggaaggc
    10141 tccatctggc catatattga aacgggtatc cttgatgaag ttttgcgcca tcgctcgacc
    10201 attgtcttta ctaattcacg cgggctggcg gaaaaactga cggcacgatt aaatgagctt
    10261 tacgccgcac gcttacagcg ttccccatct atcgccgttg atgcggccca tttcgagtcg
    10321 acctccggcg caacctctaa ccgtgtacaa agtagcgaca tttttattgc ccgctcacac
    10381 cacggctccg tctctaaaga acaacgagca atcaccgaac aggcgttgaa atcgggtgaa
    10441 ttacgctgtg tggtcgcaac ctccagtctc gaactgggga ttgatatggg cgcggtggat
    10501 ctggtgattc aggtggcaac gccgctttct gttgccagtg ggttacaacg cattggtcgc
    10561 gccggacatc aggttggcgg tgtatctaaa gggctttttt tcccccgaac caggcgtgac
    10621 ttagtcgatt ccgcagtcat tgtagagtgt atgttcgcag gcaggctgga aaacctgaca
    10681 ccgccgcata atccactcga cgtccttgcg cagcaaaccg ttgccgccgc ggcgatggat
    10741 gcattacagg cagacgaatg gtactcccgc gtacgccgtg ccgcaccgtg gaaagatctg
    10801 ccaagacgtg tttttgacgt cacgctggat atgctttccg ggcgctatcc ctctggcgat
    10861 ttttctgctt ttcgaccaaa actggtctgg aacagggaga ccgggatatt gaccgcccga
    10921 cctggcgctc agctgttggc ggttaccagc ggtggcacca ttccggatcg tggcatgtat
    10981 agcgtgttat tacccgaagg tgaagaacag gccggttcgc ggcgggtggg tgaactggat
    11041 gaggagatgg tatatgagtc gcgggtgaac gacattatca ctctcggcgc tacctcatgg
    11101 cggatccagc aaatcacccg cgatcaggtg attgtgactc ctgctccagg tcgttctgcc
    11161 cgccttccct tctggcgtgg tgaaggtaac ggacgtccgg cagaattagg cgagatgatc
    11221 ggcgattttc tccatttact ggcggatggc gcgttctttt ccgggactat tcccccgtgg
    11281 ttggcagaag aaaatacgat cgccaatatt caggggttga ttgacgagca gcgcaacgcg
    11341 acgggcatcg ttccggggag tcgtcatctg gtcctcgaac ggtgccgtga tgaaattggt
    11401 gactggcgta ttattttgca ctctccctat ggaagacggg tgcatgaacc ctgggcgctg
    11461 gcgattgccg ggcgaataca tgcactatgg ggcgctgacg cgtctgtggt tgccagtgat
    11521 gacggcattg ttgcgcgcat tcctgacacc gatggtaaat tgcctgatgc cgcgattttt
    11581 ttgtttgaac cagaaaagtt gctgcaaatt gtccgcgagg cggtaggcag ctcggcactt
    11641 ttcgccgccc gttttcgcga atgcgccgcg cggacattat taatgccggg ccgcacgccg
    11701 ggccatcgca ccccgctctg gcaacaacgg ctgcgcgcca gtcagttgct ggaaatcgct
    11761 cagggatatc cagattttcc ggtcattctc gaaaccctac gcgaatgtct gcaagatgtt
    11821 tatgatcttc ccgcgctgga acgtttgatg cgtcgcctga acggtggcga aattcaaata
    11881 tccgatgtaa cgaccactac gccttcgcct ttcgccgcca gtttattgtt cggctatgtc
    11941 gcggaattta tgtaccagag cgacgccccg ctggcagagc gccgggcatc cgtactgtcg
    12001 ctggacagcg agttactgcg caatctactc ggacaggtcg atccggggga attactcgac
    12061 ccacaggtca ttcgccaggt ggaagaagag ttgcaacgac tggctcctgg cagaagagcg
    12121 aaaggtgaag aaggattgtt cgacctgctg cgcgaactgg ggccaatgac cgttgaagac
    12181 ctggcgcaac gacatacagg cagcagtgaa gagattgcgt cgtatctgga aaatcttctt
    12241 gcagtaaaac gaatcttccc gaccatgatt agcgggcagg agcgtcttgc ctgtatggat
    12301 gatgccgcca ggctgcgcga tgctctcggc gtacgactac cagagtcact gccagagatt
    12361 tatttgcacc gagtcagtta cccgcttcgc gacctctttc tgcgctatct ccgggctcat
    12421 gctctggtca cgtctgaaca actggctcat gagtttagtc tcggtattgc cattgtcgaa
    12481 gagcaacttc agcaactgcg cgaacagggg ctggtgatga atctgcaaca agacatctgg
    12541 gtgagcgatg aagtatttcg tcgtctgcgt ttgcgctcgc tgcaagccgc cagagaagcg
    12601 acgcgtcccg ttgcggccac gacctatgcg cgattgctgc tggaacgtca gggcgtatta
    12661 cccgccaccg atggtagccc ggcactcttt gcctcaacat cgccaggcgt ttatgagggc
    12721 gtggatggcg tcatgcgggt gatcgaacag cttgccggag tcggtttacc cgcctcactc
    12781 tgggaaagcc agatcctgcc tgcccgcgta cgcgactatt cgccagaaat gctcgatgaa
    12841 ttactggcaa ccggtgcggt tatctggtcg gggcaaaaaa agctgggtga agatgacggt
    12901 ctggtggcac tgcatctaca ggaatatgcc gcagaatcgt tcactcccgc cgaagcgaat
    12961 caggcgaata gttcggcgct gcagcaagcg atagtcacgg ttctggctga cggaggagcc
    13021 tggtttgcac aacaaatcag ccaacggata cgcgataaaa tcggcgaatc ggttgatccc
    13081 tcagccatgc aagaggcgtt atgggcgctg gtctggcaag gcgtcatcac cagcgacatt
    13141 tgggcaccgt tacgcgccct cacccgcagc agtaccaaca cacgcacctc aactcgccgc
    13201 agtcaccggg ctcgtcgtgg acgtcctgtc tatgcgcaac ccgtctcgcc gctggtatct
    13261 tacaacacac caaagcttgc tggacgctgg tcgttattgc aggtggagcc actaaacgat
    13321 accgaaagga tgctggcgct ggcggaaaat atgctcgacc gctacggcat catcagtcgt
    13381 caggcggtga tagccgaaaa tatccctggc gggtttccat cgatgcaaac gctttgtcga
    13441 agtatggaag actccgggcg aattatgcga ggtcgttttg tagaaggtct gggtggcgcg
    13501 caattcgctg aacgtctgac tattgaccga ttgcgcgatc tggcgacaca agccacgcaa
    13561 acgcgccact atacaccagt ggcgctctct gccaacgatc cggctaatgc atggggaaat
    13621 cttctgccct ggcctgcaca tccggcaacg ctgataccaa cgcgtcgggc gggtgcgctg
    13681 gtggtcgttt ctggcggcaa attgttactc tatctggcgc aaggtggcaa aaaaatgctg
    13741 gtctggcagg aaaaagagga attactcgcc ccagagattt tccacgcgct gactaccgca
    13801 ctgcgtcgcg aaccacggct gcgctttacg ctaacagaag tgaatgacct accggtccgg
    13861 caaacgccga tgtttacgct gctgcgtgag gcgggatttt caagttcgcc acaagggctg
    13921 gattggggat agagaaagga ctgacgaatg cccgttcgca tccgtcagta ttgcaggacg
    13981 gattattcag cgtccggctc ttcagacttg tatttagcgg cagtttcttt gatcagctgc
    14041 tgcagttcgc cacgctgata catttcgatc acgatatcac aaccgccgac cagctcgccg
    14101 tcaacccaca gttgcgggaa ggtcggccag ttagcatatt tcggcagttc cgcacgaatg
    14161 tccggattct gcaggatatc aacataggca aaacgttcgc cacatgcggc aagcgcctgg
    14221 actgcctggg cagagaaacc gcagctcggc agtttcggtg aacctttcat gtacagcagg
    14281 atcgggtttt cagcaatctg gcgttggatt ttttcgatag tggtgctcat tgtcttgctt
    14341 cctcaaactt ctttacggca gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa
    14401 taacattttc atcacgcttt tgctatttta tccctttgct ctatttgttg catttcaaat
    14461 attcgtttct tccctgacgc acggcaaact ggcgtattta taaccatttt tagctttcac
    14521 tgctgcattt ttttgcgctc gccaacgaaa cgtatttttt aacaataaaa gctattaact
    14581 ttctcttctt ctatgcatta gaatcatcaa gttttgtaaa tcagacgcag gcatgataga
    14641 cctgccttaa cagagggacg ctcagtggcg cggataaacc gtatttcgat cacgctctgt
    14701 gctttacttt ttaccaccct gcctttaacg cctatggccc atgcttcaaa gcaagccagg
    14761 gagagttctg ctactactca tatcaccaaa aaagcagata aaaagaaaag cacggcaacc
    14821 accaaaaaga cccagaaagc cagccagaaa acagcgaaaa aagccgccag taaaagtacg
    14881 accaaaagca aaaccgcttc ttccgttaaa aaatcttcca ttaccgcttc taaaaacgcc
    14941 aaaactcgca gcaaacatgc cgtcaataaa acggcctcag ccaactttac cgaaaagtgt
    15001 accaaacgta agggctataa atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc
    15061 cttgccgacg cgcacaaagc gaaggtgcaa aaagccacga aagtggcaat gaataaactg
    15121 atgcaacaaa ttggtaagcc atatcgttgg ggcggcagct caccgcgtac cggttttgat
    15181 tgcagcggcc tggtttatta cgcttataaa gatttggtga aaattcgtat tccgcgtacg
    15241 gcgaatgaaa tgtatcacct gcgtgatgca gcacctatcg aacgtagtga actgaaaaac
    15301 ggcgacctgg tcttcttccg tactcaggga cgcggtacag ccgatcatgt cggcgtgtat
    15361 gtcggcaacg gcaaatttat tcagtcaccg cgcacgggcc aagaaattca aatcacttct
    15421 ctcagtgaag actactggca gcgccactat gttggcgctc gtcgggtaat gaccccaaaa
    15481 acacttcgct aaaactttac cctgttgtta cggcaacagg gtaagttcat cttttgtctc
    15541 accttttaat ttgctaccct atccatacgc acaataaggc tattgtacgt atgcaaatta
    15601 ataataaagg agagtagcaa tgtcattcga attacctgca ctaccatatg ctaaagatgc
    15661 tctggcaccg cacatttctg cggaaaccat cgagtatcac tatggcaagc atcatcagac
    15721 ttatgtcact aacctgaaca acctgattaa aggtaccgcg tttgaaggta aatcactgga
    15781 agagattatt cgcagctctg aaggtggcgt attcaacaac gcagctcagg tctggaacca
    15841 tactttctac tggaactgcc tggcaccgaa cgcgggtggc gaaccgactg gaaaagtcgc
    15901 tgaagctatc gccgcatctt ttggcagctt tgccgatttc aaagctcagt ttactgatgc
    15961 agcgatcaaa aactttggtt ctggctggac ctggctggtg aaaaacagcg atggcaaact
    16021 ggctatcgtt tcaacctcta acgcgggtac tccactgacc accgatgcaa ctccgctgct
    16081 gaccgttgat gtctgggaac acgcttatta catcgactat cgcaatgcac gtcctggcta
    16141 tctggagcac ttctgggcgc tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata
    16201 ataactgatg gcaaatgcaa cattacctga tgcgcttcgc gcgccatgcc aacacggatg
    16261 aggcgttaac agcgcattcg gcaagataaa tcgcacgttg ccagcaactg taacgcagaa
    16321 ggttatcctt ctgcgttttt gtttaattag ctgttggcaa cgcaaactgt ttcaggttgt
    16381 tttctggctg acataaacac cagcaataat gccagtcccg cgacaatcgc ccccatcacc
    16441 ggcacaaagc tgtatcccag cccagcggaa attaccgcac caccagcagc tgctcccagc
    16501 gcatttccaa gattaaaggc accaatattg actgatgaag acagacctgg cgcttcactg
    16561 gcgacacgca tcacgcgcat ctgtaacggc ggtacgaccg caaaggttgc agcgccccac
    16621 accaccatgc taatagctgc gccgaactca ttgcgggcca ggaacgggat tgccagcata
    16681 atcaccatca gcagcaacaa aaagcctttc aacgtgccgt taactgaacg atctgccagt
    16741 ttgccgccga gatagttacc gatagagaat ccgacaccaa tcagcaccag cattgctgtg
    16801 acgaacaccg gtgttgcgtg ggtaatactt tgcagtaccg gagagatata ggtgtagagg
    16861 gtaaacattg caccagctcc cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc
    16921 attaataccg ccagctcttt tttcacttca ggtcgtgccc ctgcaccacc tttaggtaat
    16981 gagaagaaca gacttaccat tgaaatcact cccagccccg ccgttgccag aaatgacatc
    17041 cgccagccga tggtttcacc caaccaggtc gccgccggca cgccaccgat atttgccagg
    17101 gttaacccca taaacatagt ggcaactgcg ctggcctgtt tatgttttgg caccacgctt
    17161 gcggccacga ctgaacccaa accaaaaaat gccccgtgat tcaggctggt caaaatgcgt
    17221 gaaagcatca gggtcatata atccggcgcg atggcggaaa gtacgttgcc aagcgtgaaa
    17281 atcgccatca ggaaaatcaa cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc
    17341 agcggcgcgc caaccattac gccaactgca taagcactga ttaacattcc ggcagcggga
    17401 atcgagacat ccacaccgcg cgcaatgacg ggcaacaagc ccattggcga gaactccgtt
    17461 gtcccgatac caaacgcgcc aatcgccagc gccagcaacg gatagttaat tttcatgcct
    17521 tatctccacc tcttcgcgtc attacgcgat attcattaaa gtggcgaaag catgacagca
    17581 atcacaaaaa aatgaaaata acaaaaagag aaaacacttt tgccattttg ctaacaaaca
    17641 ggaaggagat gcgagggaga acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc
    17701 aaacccacga ccacgatcaa accgaggacg ataatcgttg ttaccagtga aaatttaagg
    17761 tcggtgctca tcaagttttc tcctttttta ttaccacaca aaaagtgata ttacgcattt
    17821 ttacacactg tgatgaaaaa atctcccgtc atttataatg ataagtgttt ttaccacttc
    17881 cccttttcgt caagatcggc caaaattcca cgcttacact atttgcgtac tggccattga
    17941 ccccttcctg acgctccgtg tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa
    18001 ggtgtgtaaa ggcaaacgtt taccttgcga ttttgcagga gctgaagtta gggtctggag
    18061 tgaaatggaa tggcaacaat aaaagatgta gcgaaacgag caaacgtttc cactacaact
    18121 gtgtcacacg tgatcaacaa aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg
    18181 gcagcgatta aagaattaca ctactcccct agcgcggtgg cgcgtagcct gaaggttaac
    18241 cacaccaagt ctatcggttt gctggcgacc agcagcgaag cggcctattt tgccgagatc
    18301 attgaagcgg ttgaaaaaaa ttgcttccag aaaggttaca ccctgattct gggcaatgcg
    18361 tggaacaatc ttgagaaaca gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat
    18421 ggtctgctgg tgatgtgttc tgagtaccca gagccgttgc tggcgatgct ggaagagtat
    18481 cgccatatcc caatggtggt gatggactgg ggtgaagcaa aagctgactt caccgatgcg
    18541 gtcattgata acgcattcga aggcggctac atggccgggc gttatctgat tgaacgcggt
    18601 catcgcgaaa tcggcgttat ccctggcccg ctggaacgta acaccggcgc aggccgcctt
    18661 gccggtttta tgaaggcgat ggaagaagcg atgatcaaag tgccggaaag ctggattgta
    18721 cagggtgact ttgaacctga atccggttat cgcgccatgc agcaaatact gtcgcaaccg
    18781 catcgcccta ctgccgtctt ctgtggtggc gatatcatgg caatgggcgc actttgtgct
    18841 gctgatgaaa tgggtctgcg cgtgccgcag gatgtttcgc tgatcggtta tgataacgtg
    18901 cgcaacgccc gctattttac gccagcgctg accacgatcc atcagccaaa agattcgctg
    18961 ggcgaaacgg cgttcaacat gctgttggat cgtatcgtca ataaacgcga agaaccgcag
    19021 tccattgaag tgcatccgcg cttgattgaa cgccgctccg tggctgacgg cccgttccgc
    19081 gactatcgtc gttaatcacc cgttgcggga gtctcttccg gctcccgcag ccactcctta
    19141 ttcagcgtct cactatcgcc gagatactca agcaaccagg ttaacgcagg cgacatatca
    19201 ttttgctgcc atgtcagaca acatgccgaa tccgggaagg ggttttccag ttctagtgct
    19261 acccacttcc cctcattaag ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat
    19321 aatcctgccg agatacaggt tgccgatgat tcccagtcag gcacgacaag tcttttttgg
    19381 ttatccagca accaggtaat acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac
    19441 gacggccagt tgcgcaacgt atcatcgctg aacgggccat ccatcaacgc cagcgggtgg
    19501 tggctggcaa caacgcaact ccagcttagc atccccatat cgcggaaggc ataacgaccg
    19561 cctaccggaa tcgcgcgtgt tgcgccaatc gccagttcca cgcgcccgtc ggaaagcgca
    19621 tcccagacac cgttgaacac ttcctgaaag acaagaagtt cgacatcatc aaaatggcga
    19681 taaaaatcaa cgatcatctg ccgtgtacgt tctggcctga caatattatc cactgcgata
    19741 gctaactgac cgcgccagcc gttcgctatc tgctgacatt gctgccgggt gatctgcatt
    19801 tttttgacaa cagagcgccc ttctttgaga aaccacgccc cggcagcggt cagttccaca
    19861 tcacggtgac gtcgttcaaa cagcggcacc gccagccact cttccagctg acgcacggta
    19921 tagctgaccg cagaaggaac gcgatgcaac tcctgtgccg cagcgctaaa actaccatta
    19981 cgcgctaccg catcaacaac ttcgagtgaa tattctgacc acatagtctg cctgcaaaat
    20041 ttttgaaacc agtcatcaaa tattaccgtt tcacaacact aatttcactc cctacacttt
    20101 gcggcggtgt ttaattgaga gatttagaga atatacatgc aacctgggaa aagattttta
    20161 gtctggctgg cgggtttgag cgtactcggt tttctggcaa ccgatatgta tctgcctgct
    20221 ttcgccgcca tacaggccga cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt
    20281 ctgttccttg ccggttttgc cgcagcccag cttctgtggg ggccgctctc cgaccgttat
    20341 ggtcgtaaac cggtattatt aatcggcctg acaatttttg cgttaggtag tctggggatg
    20401 ctgtgggtag aaaacgccgc tacgctgctg gtattgcgtt ttgtacaggc tgtgggtgtc
    20461 tgcgccgcgg cagttatctg gcaagcgtta gtgacggatt attatccttc acagaaagtt
    20521 aaccgtattt ttgcgaccat catgccgctg gtgggtctat ctccggcact ggctcctctg
    20581 ttaggaagct ggctgctggt ccatttttcc tggcaggcga ttttcgccac cctgtttgcc
    20641 attaccgtgg tgctgattct gcctattttc tggctcaaac ccacgacgaa ggcccgtaac
    20701 aatagtcagg atggtctgac ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac
    20761 gtgctgatat atgcggcctg ttcagccagt ttttttgcat ggctgaccgg ctcaccgttc
    20821 atccttagtg aaatgggcta cagcccggca gttattggtt taagttatgt cccgcaaact
    20881 atcgcgtttc tgattggtgg ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag
    20941 cagttattac cgtggttgct ggtactgttt gctgtcagcg tcattgcgac ctgggctgcg
    21001 ggcttcatta gccatgtgtc gctagtcgaa atcctgatcc cattctgtgt gatggcgatt
    21061 gccaatggcg cgatctaccc gattgttgtc gcccaggcgc tgcgtccctt cccacatgca
    21121 accggtcgcg ccgcagcgtt gcagaacact ctacaactgg gtctgtgctt cctcgcaagt
    21181 ctggtagttt cctggctgat cagtatcagc acgccattgc tcaccaccac cagcgtgatg
    21241 ttatcaacag tagtgctggt cgctctgggt tacatgatgc aacgttgtga agaagtagac
    21301 tgtcagaatc atggcaatgc cgaagtcgct catagcgaat cacactgatc tatatcgata
    21361 tgattatact taggctgcta acaaaatttt gttgtatgat tgaaattagc ggcctatact
    21421 aatttcgagt tgttaaagct acgataaata ttatgttttt acggggacag gatcgttccc
    21481 gactcactat ggatggtcat ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga
    21541 ttatagactc gcggtttttt ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg
    21601 ttttaaggtt ctgatcaccg accagtgatg gagaaactat gagttcatcg tgtatagaag
    21661 aagtcagtgt accggatgac aactggtacc gtatcgccaa cgaattactt agccgtgccg
    21721 gtatagccat taacggttct gccccggcgg atattcgtgt gaaaaacccc gattttttta
    21781 aacgcgttct gcaggaagga tcgttggggt taggcgaaag ttatatggat ggctggtggg
    21841 aatgtgaccg actggatatg ttttttagca aagtcttacg cgcaggtctc gagaaccaac
    21901 tcccccatca tttcaaagac acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga
    21961 gtaaaaaacg tgcctggata gtcggcaaag agcattacga tttgggtaat gacttgttca
    22021 gccgcatgct tgatcccttc atgcaatatt cctgtgctta ctggaaagat gccgataatc
    22081 tggaatcagc ccagcaggcg aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag
    22141 ggatgcgcgt actggatatt ggctgtggct ggggtggact ggcgcactac atggcgtcta
    22201 attatgacgt aagcgtggtc ggcgtcacca tttctgccga acagcaaaaa atggctcagg
    22261 aacgctgtga aggcctggat gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc
    22321 agtttgatcg tattgtttct gtggggatgt tcgagcacgt cggaccgaaa aattacgata
    22381 cctattttgc ggtggtggat cgtaatttga aaccggaagg catattcctg ctccatacta
    22441 tcggttcgaa aaaaaccgat ctgaatgttg atccctggat taataaatat atttttccga
    22501 acggttgcct gccctctgta cgccagattg ctcagtccag cgaaccccac tttgtgatgg
    22561 aagactggca taacttcggt gctgattacg atactacgtt gatggcgtgg tatgaacgat
    22621 tcctcgccgc atggccagaa attgcggata actacagtga acgctttaaa cggatgttta
    22681 cctattatct gaatgcctgt gcgggtgctt tccgcgcccg tgatattcag ctctggcagg
    22741 tcgtgttctc acgcggtgtt gaaaacggcc ttcgagtggc tcgctaaagg ctattctatc
    22801 gccccctctc cgggggcgat ttcagatcag gcttctgtac ctggttgatt catggcattt
    22861 tctcgtgccg ccagcactcg ttctaccgta tctaccactg cctgagtttg tggatcgatt
    22921 tcaatgttga cgcgtgcgcc aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc
    22981 ggaattaaat ggacgcaaaa acgcgttggc gtgacttcac cgacggtcag gctaataccg
    23041 tcgatgccaa taaatccttt gtacagaata tatttcatca actgactatc ctggacttta
    23101 aaccagatct ggcgattatt ttccgaggtt aatattttcg ccacttcagc ggtggtcata
    23161 atatgacctg acattaagtg tccgccaatt tcatcactga atttcgccgc acgctcaacg
    23221 tttacccaat cccccacttt taaatcgcca agattggtaa tgcgtaacgt ttctttcatc
    23281 aggtcaaaac tgacatggtt gccgttaatt tccgtcacgg tcaggcagca accgttatgc
    23341 gccacggaag caccggtttc cagaccgtcc agcatgtggt cgggtaactc caccacatgt
    23401 gtgcgaaaat ttggtttctc gtcaatcgac accagttttg cggtgccctg tacaatcccc
    23461 gtaaacatac ttacaactcc tgaaatcagt taagacattc tgttcagcac aatagcaggt
    23521 ggcaaacgcc cttaccagtg aaggggtaag aatgactatt ttttcactgg agaattaata
    23581 aatcctcgct acaatagact gaatttcccc tgcttcttct ttttgctgcc cattcaggcg
    23641 gctttttagt ctctcatata actacaaata aaaggtgttc acgtgcagaa gtatatcagt
    23701 gaagcgcgtc tgttattagc attagcaatc ccggtgattc tcgcgcaaat cgcccaaact
    23761 gcgatgggtt ttgtcgatac cgtgatggcg ggcggctata gtgccaccga catggcggcg
    23821 gtcgctatcg gtacttctat ctggcttccg gcgatcctct ttggtcacgg actgctgctg
    23881 gcattaacgc cggttatcgc gcaattaaat ggttccggtc gacgtgagcg cattgcgcat
    23941 caggtgcgac aaggtttctg gctggcgggt tttgtttccg ttctcattat gctggtgctg
    24001 tggaatgcag gttacattat ccgctccatg gaaaacatcg atccggctct ggcggacaaa
    24061 gccgtgggtt atctgcgtgc gttgctatgg ggcgcgccgg gctatctttt cttccaggtt
    24121 gcccgtaacc agtgtgaagg tctggcaaaa accaagccgg gtatggtgat gggctttatc
    24181 ggcctgctgg tgaacattcc ggtgaactat atctttattt acggtcattt cggtatgcct
    24241 gagctcggtg gcgttggttg tggcgtggct actgctgcgg tgtattgggt catgttcctt
    24301 gccatggttt cttacattaa acgcgcccgc tcgatgcgcg atattcgtaa cgaaaaaggc
    24361 actgcgaagc ccgatcctgc ggttatgaaa cgactgattc aactcggttt gccaattgcg
    24421 ctggcgctgt tctttgaagt aacgctgttt gccgtcgtgg ctctgttagt gtctccgctc
    24481 ggtattgttg atgtcgcagg acatcagatt gccctgaact ttagttcact gatgttcgta
    24541 cttcccatgt cgctggcggc agcggtaacc atccgcgtag gttatcgtct gggtcagggc
    24601 tcaacgctgg atgcgcaaac cgctgcgcgg accgggctta tggtgggtgt ctgtatggca
    24661 accctgacgg ccattttcac ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac
    24721 aatcccgagg ttgtaacgct ggctgcgcat ttgatgttgc tggcggcggt atatcagatt
    24781 tctgactcaa tccaggtgat tggcagtggg attttgcgtg gatataaaga tacgcgttcc
    24841 attttctata ttacctttac ggcttactgg gtgctgggct tgccaagcgg ctatattctg
    24901 gcactgaccg atctggtcgt tgaacctatg gggccagcag gcttctggat aggctttatt
    24961 attggcctga cgtcggcagc cattatgatg atgctgcgta tgcggttcct gcaacgtctg
    25021 ccgtcagcca tcattctgca acgagcagct cgctagtaaa gacaaggcgc aaccttcacg
    25081 ggttgcgcct gtgtttttac gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct
    25141 gtagtttcag tgcgttacac agagtttgcc gactggcatc tgttatctca acgtgtatat
    25201 caccagtaac ttcccctttc acattgatga agtcaggtga aatttctttc gcactggttt
    25261 gcaggcaaat cgctccctct ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg
    25321 caccagtgat tagccacagt tttccgccat ttagattgat gctaccaaca ctgaaagtga
    25381 cactgtcacc ttttaccgta ctgccgtcga gaatcaatac accgccctgc tgtacagtaa
    25441 tcgttgattg ttgcagatct gcgccatagc caataatcat tgaagcgccg tccagtaatg
    25501 caacactacc ggtcagttca accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg
    25561 cgctggtggg tgacaacagg atgactgtcc caccttgtat taccgcattt tccagcgtac
    25621 cggcatgcac ttcagcccga ccacccacat taacggacag gttttgcgtc ttaccggaac
    25681 tgtagagctg aagaccatcc gtccccagac gataagtgga tccattctca acaatagtgt
    25741 cagagtcttc accaccgaga ttattcatgg tgccaccagc aagcaatgta tcactggctt
    25801 tccccgccag cataaaaacg ccgccctgat taatctcaca attgctggct tccccacctg
    25861 gatacaccaa caattcacca ccttcatcta ccacgaccgc tgaggttgtc ccattgacca
    25921 acaggccacc ctcttgatcg agaataattt tttccgcgcg atggccttcc agtacacgca
    25981 ggttaccgcc attttccagc aacaaaccgc aggcataacc tttatcaacg ctgaattcac
    26041 cttcgggatg gcggccatta accgtagcga gcgtagagag actaattgcg cctccctcgg
    26101 actgaacaat atctgtggca atggcaccgt cgctgaccga tagcgtgcca ttgttgccgt
    26161 actgtgtgca tttggcgatc ccaccggttg ccactttcaa ccagccagcc cccgtgacca
    26221 cggtattttc ggctcttccg ccagcttcaa caattaactc accttcagta agttgcgtcg
    26281 cgccaatgac ctcgccggtc ttaacaattt gcacgttccc gtcgctcatc aagtttttcg
    26341 catcagatcc catgatttat tcctttgctg catctgtgtg cctttattgc tacctaagtg
    26401 taaaggctac ggaggattta tccacgacag attttgagat ggtggcaaac aactctgttt
    26461 aaactctgat acacggatta ttgggttgta tctgatgtaa atgcgatcct gaataaaaat
    26521 cgcccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa
    26581 tagcgcagaa aattacgttt tgcctcttgc caccttccca ctctgccgct aatattcgtc
    26641 cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat
    26701 ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg
    26761 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag
    26821 attttcttaa tctggtcttc tcctttttcc ctctgtttct tctctatatc caatacgtta
    26881 aaagatttac actgtcttcg tatgcgttat cagaaggaga atcgctatgg caactttgtt
    26941 acaacttcat ttttctttca atggcccatt tggcgacgca atggctgagc agcttaaacc
    27001 gcttgctgag tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga
    27061 aaagaaccac gaagccggtg ggatctacct ttttactaat gaaaaaagcg cccttgccta
    27121 tctggaaaaa catactgccc gactgaaaaa tctcggcgtt gaggaagttg tcgccaaagt
    27181 tttcgatgtc aatgagccac ttag
//
LOCUS       DAZGAP010000057        27021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGAP010000057 DAZGAP010000000
VERSION     DAZGAP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.057"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..1625
                     /gene="casA"
                     /locus_tag="ACHLAM_002003"
     CDS             117..1625
                     /gene="casA"
                     /locus_tag="ACHLAM_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO7853530.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LATLALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVCGIDLRSTVLLNVLTIPRLQKQFPNELHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
     gene            1618..2100
                     /gene="casB"
                     /locus_tag="ACHLAM_002004"
                     /gene_synonym="cse2"
     CDS             1618..2100
                     /gene="casB"
                     /locus_tag="ACHLAM_002004"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO7853531.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QSFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKTEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            2113..3204
                     /gene="cas7e"
                     /locus_tag="ACHLAM_002005"
     CDS             2113..3204
                     /gene="cas7e"
                     /locus_tag="ACHLAM_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO7853532.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KHAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKLVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITAQVKQMPTLEQLKSWVRNNGEA"
     gene            3207..3881
                     /gene="cas5e"
                     /locus_tag="ACHLAM_002006"
     CDS             3207..3881
                     /gene="cas5e"
                     /locus_tag="ACHLAM_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO7853533.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            3868..4467
                     /gene="cas6e"
                     /locus_tag="ACHLAM_002007"
     CDS             3868..4467
                     /gene="cas6e"
                     /locus_tag="ACHLAM_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO7853534.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            4483..5400
                     /gene="cas1e"
                     /locus_tag="ACHLAM_002008"
     CDS             4483..5400
                     /gene="cas1e"
                     /locus_tag="ACHLAM_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO7853535.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYVLLAKQYGVKW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRIPGEPDREVRLACRDIFRSSKTLVKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            5402..5686
                     /gene="cas2e"
                     /locus_tag="ACHLAM_002009"
     CDS             5402..5686
                     /gene="cas2e"
                     /locus_tag="ACHLAM_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO7853536.1"
                     /translation="MSMLVVVTENVPSRLRGRLAIWLLEVRAGVYVGDVSARVREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
     repeat_region   5792..6186
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5794..5820
                     /rpt_unit_seq="gttccccgcgccagcggggataaaccg"
     gene            complement(6268..7305)
                     /gene="iap"
                     /locus_tag="ACHLAM_002010"
     CDS             complement(6268..7305)
                     /gene="iap"
                     /locus_tag="ACHLAM_002010"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO7853537.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            7557..8465
                     /gene="cysD"
                     /locus_tag="ACHLAM_002011"
     CDS             7557..8465
                     /gene="cysD"
                     /locus_tag="ACHLAM_002011"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO7853538.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            8467..9894
                     /gene="cysN"
                     /locus_tag="ACHLAM_002012"
     CDS             8467..9894
                     /gene="cysN"
                     /locus_tag="ACHLAM_002012"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO7853539.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            9894..10499
                     /gene="cysC"
                     /locus_tag="ACHLAM_002013"
     CDS             9894..10499
                     /gene="cysC"
                     /locus_tag="ACHLAM_002013"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO7853540.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            10549..10872
                     /gene="ygbE"
                     /locus_tag="ACHLAM_002014"
     CDS             10549..10872
                     /gene="ygbE"
                     /locus_tag="ACHLAM_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO7853541.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            11065..11376
                     /gene="ftsB"
                     /locus_tag="ACHLAM_002015"
     CDS             11065..11376
                     /gene="ftsB"
                     /locus_tag="ACHLAM_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO7853542.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            11395..12105
                     /gene="ispD"
                     /locus_tag="ACHLAM_002016"
     CDS             11395..12105
                     /gene="ispD"
                     /locus_tag="ACHLAM_002016"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO7853543.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            12105..12584
                     /gene="ispF"
                     /locus_tag="ACHLAM_002017"
     CDS             12105..12584
                     /gene="ispF"
                     /locus_tag="ACHLAM_002017"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO7853544.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            12581..13630
                     /gene="truD"
                     /locus_tag="ACHLAM_002018"
     CDS             12581..13630
                     /gene="truD"
                     /locus_tag="ACHLAM_002018"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO7853545.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQKLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            13611..14372
                     /gene="surE"
                     /locus_tag="ACHLAM_002019"
     CDS             13611..14372
                     /gene="surE"
                     /locus_tag="ACHLAM_002019"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO7853546.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            14366..14992
                     /gene="pcm"
                     /locus_tag="ACHLAM_002020"
     CDS             14366..14992
                     /gene="pcm"
                     /locus_tag="ACHLAM_002020"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO7853547.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            15177..16271
                     /gene="nlpD"
                     /locus_tag="ACHLAM_002021"
     CDS             15177..16271
                     /gene="nlpD"
                     /locus_tag="ACHLAM_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO7853548.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            16334..17326
                     /gene="rpoS"
                     /locus_tag="ACHLAM_002022"
     CDS             16334..17326
                     /gene="rpoS"
                     /locus_tag="ACHLAM_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO7853549.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(17447..17854)
                     /locus_tag="ACHLAM_002023"
     CDS             complement(17447..17854)
                     /locus_tag="ACHLAM_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO7853550.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            18001..18594
                     /locus_tag="ACHLAM_002024"
     CDS             18001..18594
                     /locus_tag="ACHLAM_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO7853551.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            18594..20021
                     /locus_tag="ACHLAM_002025"
     CDS             18594..20021
                     /locus_tag="ACHLAM_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO7853552.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFNILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            20032..20268
                     /locus_tag="ACHLAM_002026"
     CDS             20032..20268
                     /locus_tag="ACHLAM_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO7853553.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(20306..21670)
                     /gene="ygbN"
                     /locus_tag="ACHLAM_002027"
     CDS             complement(20306..21670)
                     /gene="ygbN"
                     /locus_tag="ACHLAM_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO7853554.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCI
                     WAVI"
     gene            complement(21759..22535)
                     /gene="ygbM"
                     /locus_tag="ACHLAM_002028"
     CDS             complement(21759..22535)
                     /gene="ygbM"
                     /locus_tag="ACHLAM_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO7853555.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(22540..23178)
                     /gene="ygbL"
                     /locus_tag="ACHLAM_002029"
     CDS             complement(22540..23178)
                     /gene="ygbL"
                     /locus_tag="ACHLAM_002029"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO7853556.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(23175..24437)
                     /gene="otnK"
                     /locus_tag="ACHLAM_002030"
     CDS             complement(23175..24437)
                     /gene="otnK"
                     /locus_tag="ACHLAM_002030"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO7853557.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(24434..25342)
                     /gene="ltnD"
                     /locus_tag="ACHLAM_002031"
     CDS             complement(24434..25342)
                     /gene="ltnD"
                     /locus_tag="ACHLAM_002031"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO7853558.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            25538..26305
                     /gene="ygbI"
                     /locus_tag="ACHLAM_002032"
     CDS             25538..26305
                     /gene="ygbI"
                     /locus_tag="ACHLAM_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO7853559.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            complement(26356..27012)
                     /gene="pphB"
                     /locus_tag="ACHLAM_002033"
     CDS             complement(26356..27012)
                     /gene="pphB"
                     /locus_tag="ACHLAM_002033"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7853560.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
BASE COUNT         6706 a         6613 c         7158 g         6544 t
ORIGIN      
        1 gtgaatgtga ttattgaatt aattacgccg tattttttct ttgtttttac cgataacgga
       61 agtgtgccga cgcatagaaa tgcaggagaa atgtcggagc atatgaagga gaacaaatga
      121 atttgcttat tgataactgg atccctgtac gcccgcgaaa cggggggaaa gtccaaatca
      181 taaatctgca atcgctatac tgcagtagag atcagtggcg attaagtttg ccccgtgacg
      241 atatggaact ggccacttta gcactgctgg tttgcattgg gcaaattatc gccccggcaa
      301 aagatgacgt tgaatttcga catcgcataa tgaatccgct cactgaagat gagtttcaac
      361 aactcatcgc gccgtggata gatatgttct accttaatca cgcagaacat ccctttatgc
      421 agaccaaagg tgtcaaagca aatgatgtga ctccaatgga aaaactgttg gctggggtaa
      481 gcggcgcgac gaattgtgca tttgtcaatc aaccggggca gggtgaagca ttatgtggtg
      541 gatgcactgc gattgcgtta ttcaaccagg cgaatcaggc acctggtttt ggtggtggct
      601 ttaaaagcgg tttacgtgga ggaacaccta taaccacctt cgtatgtggg atcgatcttc
      661 gctcgacggt attactcaat gtcctcacaa tacctcgtct tcaaaaacaa tttcccaatg
      721 aattacatac ggaaaaccaa cctacctggg ttaagcctgt caagccgaat gaatcggtgc
      781 ctgcttcttc aattgggttt gtccgtggtt tattctggca accagcgcat attgaactat
      841 gcgatcccat tgggattggt aaatgttctt gctgtggtca ggaaagcaat ttgcgttata
      901 ccggttttct taaggaaaaa tttaccttta cagttaatgg gctatggccc catccgcatt
      961 ccccttgtct ggtaacagtc aagaaagggg aggttgagga aaaatttctt gctttcacca
     1021 cctccgcacc atcatggaca caaatcagcc gagttgtggt agataagatt attcaaaatg
     1081 aaaatggaaa tcgcgtggcg gcggttgtga atcaattcag aaatattgcg ccgcaaagtc
     1141 ctcttgaatt gattatgggg ggatatcgta ataatcaagc atctattctt gaacggcgtc
     1201 atgatgtgtt gatgtttaat caggggtggc aacaatacgg caatgtgata aacgaaatag
     1261 tgactgttgg tttgggatat aaaacagcct tacgcaaggc gttatatacc tttgcagaag
     1321 ggtttaaaaa taaagacttc aaaggggccg gagtttctgt tcatgagact gcagaaaggc
     1381 atttctatcg acagagtgaa ttattaattc ccgatgtatt ggcgaatatt aatttttccc
     1441 aagctgatga ggtaatagct gatttacgag acaaacttca tcaattgtgt gaaatgctat
     1501 ttaatcaatc tgtagctccc tatgcacatc atcctaaatt aataagcaca ttagcgcttg
     1561 cccgcgccac gctatacaaa catttacggg agttaaaacc tcaaggaggg ccatcaaatg
     1621 gctgatgaaa ttgatgcaat ggctttatat cgagcctggc aacaactgga taatggagca
     1681 tgtgcgcaaa ttagacgtgt ttcagaacct gatgaattac gcgatatccc tgcgttttat
     1741 aggctggtgc aatcttttgg ttgggaaaac ccacgtcacc agcaggctct tttgcgcatg
     1801 gtgttttgcc tgagcgcagg aaagaatgtc atccgacatc aggacaaaaa aacggagcaa
     1861 acaacaggta tctcgttggg aagagcttta gccaatagtg gaagaattaa cgagcgccgt
     1921 atctttcaat taattcgggc tgacagaaca gccgatatgg tccagttacg tcgattactt
     1981 actcacgccg aacccgtact tgactggcca ttaatggcca ggatgttgac ctggtgggga
     2041 aagcgcgaac gccagcaact tctggaagat tttgtattga ccacaaacaa aaatgcgtaa
     2101 ggaaaccttt ctatgtctaa ctttatcaat attcatgttc tgatctctca cagcccttca
     2161 tgtctgaacc gcgacgatat gaacatgcag aaagacgcta ttttcggcgg gaaaagacga
     2221 gtaagaattt caagtcaaag ccttaaacat gcgatgcgta aaagtgatta ttacgcacaa
     2281 aatattggtg aatccagtct cagaacaatt catcttgcac aattacgtga tgttcttcgg
     2341 caaaaacttg gtgaacgttt tgaccaaaaa atcatcgata agacattagc gctgctctcc
     2401 ggtaaattag ttgatgaagc cgaaaagatt tctgccgatg cggttactcc ctgggttgtg
     2461 ggagaaatag cctggttctg tgagcaggtt gcaaaagcag aggctgataa tctggatgat
     2521 aaaaagctgc tcaaagttct taaggaagat attgccgcca tacgtgtgaa tttacagcag
     2581 ggtgttgata ttgcgcttag tggaagaatg gcaaccagcg gcatgatgag tgagttggga
     2641 aaagttgatg gtgcaatgtc cattgcgcat gcgatcacta ctcatcaggt tgattctgat
     2701 attgactggt tcaccgctgt agatgattta caggaacaag gttctgcaca tctgggaact
     2761 caggaatttt catcgggtgt tttttatcgt tatgccaaca ttaacctcgc tcaacttcag
     2821 gaaaatttag gtggtgcctc cagggagcag gctctggaaa ttgcaaccca tgttgttcat
     2881 atgctggcaa cagaggtccc tggagcaaaa cagcgtactt atgccgcttt taaccctgcg
     2941 gatatggtaa tggttaattt ctccgatatg ccactttcta tggcaaatgc ttttgaaaaa
     3001 gcggttaaag cgaaagatgg ctttttgcaa ccgtctatac aggcgtttaa tcaatattgg
     3061 gatcgcgttg ccaatggata tggtctgaac ggagctgctg cgcaattcag cttatctgat
     3121 gtagacccaa ttactgctca agttaaacaa atgcctactt tagaacagtt aaaatcctgg
     3181 gttcgtaata atggcgaggc gtgaacatga gatcttattt gatcttgcgg cttgctgggc
     3241 caatgcaagc ctgggggcag ccgacctttg aaggaacgcg acctaccgga agatttccga
     3301 cccgaagcgg gttattaggg ctactcgggg cttgtcttgg gatccaacgt gatgatactt
     3361 cttcattaca ggcgttatca gagagtgtgc aatttgcagt gcgctgcgat gaactcattc
     3421 ttgacgatcg tcgtgtgtct gtaacggggt tgcgtgatta ccatacagtc cttggagcgc
     3481 gagaagatta ccgtggtttg aaaagtcatg aaacgattca aacatggcgc gaatatttat
     3541 gtgatgcctc ctttaccgtc gctatctggt taacaccaca ggcaacgatg gttatgtcag
     3601 aacttgaaaa agcagtatta aagcctcggt atacaccgta tctggggcgg aggagttgtc
     3661 cgctaacaca accgcttttt ctcgggacat gtcaggcgtc ggatcctcag aaggcgctat
     3721 taaattatga gcccgttggc ggcgatattt atagtgagga atcagttgac gggcatcatt
     3781 taaaatttac ggtgcgcgac gaaccgatga tcaccttacc tcgacaattt gcttcccgag
     3841 aatggtatgt gattaaagga ggtatggatg tatctcagta aagtcatcat tgccagggcc
     3901 tggagcaggg atctttacca acttcaccag ggattatggc atttatttcc aaacagaccg
     3961 gatgctgctc gtgattttct ttttcatgtt gagaagcgaa acacaccaga aggctgtcat
     4021 gttttattgc agtcagcgca aatgcctgtt tctactgccg ttgcgacagt cattaaaact
     4081 aaacaggttg aatttcaact tcaggttggt gttccactct attttcggct tcgggcaaat
     4141 ccgatcaaaa ctattctcga caatcaaaag cgcctggaca gtaaagggaa tattaaacgc
     4201 tgtcgggttc cgttaataaa agaagcagaa caaatcgcgt ggttgcaacg taaattgggc
     4261 aatgcggcgc gcgttgaaga tgtgcatccc atatcggaac ggccacagta tttttctggt
     4321 gatggtaaaa gtggaaagat ccaaacggtt tgctttgaag gggtgatcac catcaacgac
     4381 gcgccagcgt taatagatct tgtacagcaa ggtattgggc cagctaaatc gatgggatgt
     4441 ggcttgctat ctttggctcc actgtgattg aggtgtaata aaatgacctg gcttcccctt
     4501 aatcccattc cactcaaaga tcgcgtctcc atgatctttc tgcaatatgg gcagatcgat
     4561 gtaatagatg gcgcgtttgt acttatcgac aagacaggga tccgcactca tattcctgtt
     4621 ggctcggttg cctgcatcat gttggaacct ggtacacggg tttcgcatgc agctgtacgc
     4681 ctggctgcgc aagttggaac attattggta tgggtggggg aagcgggcgt tcgtgtttat
     4741 gcttctggtc agcctggtgg tgcgcgttca gataagctgc tctatcaggc aaaacttgct
     4801 ctggatgaag atttgcgtct gaaggtcgta cgtaaaatgt ttgaacttcg gtttggagaa
     4861 cctgcgcctg cccggcgctc cgtagagcaa ctcagaggta tagaaggaag tcgtgtgcga
     4921 gcaacctatg tacttctggc gaagcaatac ggggtgaaat ggaatggacg tcgttacgat
     4981 ccgaaagact gggaaaaggg cgatacggtc aaccaatgca ttagcgctgc aacttcttgt
     5041 ttatatggcg taactgaagc ggcgatactt gcagctggtt atgcaccagc tattgggttt
     5101 gtgcatacag gaaagcctct ttcctttgtt tacgatatcg cggacatcat taaatttgac
     5161 actgttgtac cgaaagcttt tgagatagcg cgtcgtattc ctggtgagcc ggaccgggaa
     5221 gtccgtttgg cgtgcaggga tatttttcgc agtagtaaaa cattagtcaa attgattccg
     5281 cttatagagg acgtgcttgc tgctggggaa atacagccgc cggccccacc tgaagatgcg
     5341 cagcctgttg ctattccact tcccgtttct ctgggagatg ccggacatcg gagtagctga
     5401 gatgagtatg ttggtcgtgg tcactgaaaa tgtaccttcg cgcttacgag gcagattagc
     5461 catctggttg ttggaggtac gtgcaggggt gtatgtaggt gatgtatccg caagagtccg
     5521 tgaaatgatc tgggaacaaa tatctggact ggcggaagaa ggcaatgtgg tgatggcatg
     5581 ggctacgaat acggaatcgg gatttgagtt ccagacattt ggggtaaaca ggcgtactcc
     5641 ggtagatttg gatggtttaa ggttggtctc ttttttacct gtttaaaaac aaagaattag
     5701 ctgatcttta ataataagga aatgttacat taaggttggt gggttgtttt tatgggaaaa
     5761 aatgctttaa gaacaaatgt atacttttag agagttcccc gcgccagcgg ggataaaccg
     5821 gtgccgggtt aatgggggag gctggccccg aggagttccc cgcgccagcg gggataaacc
     5881 gtaaataacg gcattaccga gtgtgaaact gaagagttcc ccgcgccagc ggggataaac
     5941 cgattccgtc aaatttatca ctcaacgcca gttcgtgttc cccgcgccag cggggataaa
     6001 ccgagattgg ttttccagtc tttggcggtt ggcaagtgtt ccccgcgcca gcggggataa
     6061 accgctgtgc cggtagcccc ctccctgttc agcctcgtgt tccccgcgcc agcggggata
     6121 aaccgggtaa aaacacggtc tgaaccgaca ttcatgtgtg ttccccgcgt cagcggggat
     6181 aaaccgggcg cactggatgc gatgatggat atcacttaga attccccgcc cctgcggtag
     6241 aactcccaac tcccattttc aaacccatca agacgccttc gccaactcct tcaccagagg
     6301 tagcattatc cgcataacat cacggcagcg acgttctatt cttccaggaa gcgccttatc
     6361 aatatgttgt tgattatcca gtcttacgtc atgccagcta tttcccgcag ggaatgcggc
     6421 tgtttttgcg cgttgctgat aaccatcctt attcccaaga ttccagttag tcgcttccac
     6481 cgaaagtaca gcaataccgg ctttgtcgaa tatttctgcg tcattacaac acccagtgcc
     6541 tttcggataa tttttattca aacccggatt ggtcgttgcg gcaattccat gactacgcgc
     6601 aattgccagc gccctgtcgc gcgttaattt ccttactgct tcaggggttt ttacaccact
     6661 attgaaatac aatttatcgc caacaattaa gttatcgaga ttaatcacca gcagcgtatt
     6721 tttcttttcg gtgtcactca tccgcttgag taaattctca gcacctaatt tcccttcctc
     6781 ttcaccactg gtcgccacaa atcgaatacc atactcggta ggcgtatttt tcaggcgttc
     6841 tgccagttcc agcatgacac ctaaacctgc ggcgttatca tctattcctt gtaacgtcag
     6901 cccgccgaga ttggcatcgg cgtcagcatc gctcagcgga gcgtaagtat ccagatgcgc
     6961 cataatgatg atctgctgcg gagctttgcc ttcatgagcg gcaatcaccg tacttcccgt
     7021 cacgttatgc cagctcttgc gattatcgcg ggcggtataa atataccgac tattaaatgt
     7081 ccgaatatca ctgcgataac ccatttgctg aaactgttgg cgaatataat cggcagataa
     7141 catttctgca ggagttccgg tcatgcgtcc cggaaagaaa gtagcaatat gtcgtgcctg
     7201 ggtattagca aaatcgccag gtttaggtga cgaggcgtgt acggggagga taaagcatac
     7261 gccgagcgcc agggcagcgg tacggtggcg caatgcggaa aacatagtga gtccttaaat
     7321 accatgcaaa tttttttacc gtcatagtat gaaactgccg ctgcgctaaa acaatttcaa
     7381 atcttcctaa acgcccgaaa tccggtgcct taagcacttt ttgatattag ctttgccaaa
     7441 tcgttattcc gttaaggaac tactcattct aattggtaat ttcattcgtt ctcttacgct
     7501 ccctatagtc gaaacatctg atggcaagaa aatagcggta ttgcaaagga acggttatgg
     7561 atcaaaaacg acttacccac ctgcggcaac tggaggcgga aagcatccac attattcgcg
     7621 aggtggctgc agaattctca aatccggtga tgctctactc tatcggtaaa gattccagcg
     7681 tcatgctgca tctggcgcgc aaggcgtttt atccaggtac gctgcctttc ccgttgctgc
     7741 atgtcgatac cggctggaaa ttccgcgaga tgtatgagtt ccgcgatcgt acggcgaaag
     7801 cctacggctg cgagctgctg gtgcataaaa acccggaagg cgtggcgatg gggattaatc
     7861 cattcgtaca cggcagcgcg aaacataccg atattatgaa aactgaaggc ctgaaacagg
     7921 cgctgaacaa atacggtttt gatgccgcct tcggtggtgc gcgccgtgac gaagagaaat
     7981 cccgcgctaa agagcgaatc tactctttcc gtgaccgctt ccatcgctgg gatccgaaaa
     8041 atcagcgccc ggagctgtgg cacaactaca acgggcaaat taacaaaggc gaaagcatcc
     8101 gcgtcttccc gctctctaac tggaccgagc aggatatctg gcaatacatc tggctggaaa
     8161 atatcgacat tgttccgctg tatcttgctg cggaacgtcc ggttctggaa cgcgacggta
     8221 tgttgatgat gattgatgac aaccgtatcg acctgcaacc gggcgaagtg attaaaaaac
     8281 ggatggtgcg tttccgcaca ctgggctgtt ggccgctgac cggcgcggtg gagtcaaatg
     8341 cgcaaacact gccggagatc atcgaagaga tgctggtttc caccaccagt gaacgtcagg
     8401 gccgcgtaat tgaccgcgac caggcggggt ctatggagct gaaaaaacgt caggggtatt
     8461 tttaagatga acaccgcact tgcacaacaa atcgccaatg aaggcggcgt cgaagcatgg
     8521 atgattgcgc aacaacataa aagcctgctg cgttttctga cctgtggtag cgtcgatgac
     8581 ggcaaaagta ccctgattgg tcgcctgctg cacgataccc gccaaatcta cgaagatcag
     8641 ctctcatcgc tgcataacga cagtaagcgt cacggcaccc agggcgaaaa gctggatctg
     8701 gcactgctgg tggacggcct gcaagctgag cgcgaacagg gcatcactat tgatgtggcc
     8761 taccgctatt tctctaccga gaagcgtaaa tttattatcg ccgacacccc agggcacgag
     8821 cagtacaccc gcaatatggc gactggcgca tcgacatgtg aactggcgat cttactgatc
     8881 gatgcccgta aaggcgtgct cgatcaaacc cgtcgtcata gttttatctc cacactgttg
     8941 gggatcaaac atctggtcgt ggcgatcaac aaaatggatc tggtggatta cagtgaagag
     9001 acgttcaccc gtattcgtga agattatctg acttttgccg ggcagctgcc gggtaatctg
     9061 gatatccgct ttgtgccgct ctccgcactg gaaggcgaca acgtggcttc gcaaagtgaa
     9121 agtatgccgt ggtacagcgg tccgacactg ctagaagtgc tggaaaccgt ggagatccag
     9181 cgagtggtgg atgctcagcc aatgcgcttc ccggtgcagt acgttaaccg tccgaatctc
     9241 gattttcgtg gctacgccgg aacgctggca tccggtcgcg tggaagtcgg gcaacgtgtc
     9301 aaagtgctgc cctctggtgt ggaatcaaac gtcgcgcgga tcgtgacttt tgatggcgat
     9361 cgcgaagaag cctttgccgg agaagcgatc accctggtgc tgacggatga gatcgacatc
     9421 agccgtggcg atctgctgct ggcggcagac gaagcgttac cggctgtgca gagcgcgtcg
     9481 gtggatgtgg tatggatggc ggaacagccg ctttccccgg gccagagtta tgacatcaaa
     9541 attgccggta agaagacgcg tgctcgtgtt gatggcattc gttatcaggt tgatatcaat
     9601 aaccttaccc aacgcgaagt tgaaaacctg ccgctgaacg gcatcggcct ggtagatctc
     9661 acctttgacg agccactggt gttagatcgt tatcagcaaa acccggttac cggtgggctg
     9721 atttttatcg atcgcctgag taatgtgacc gtaggtgccg gtatggtgca cgaaccagtt
     9781 agccaggcaa ctgctgcgcc atctgaattc agtgcattcg aactggaatt gaatgccctg
     9841 gttcgccgcc atttcccgca ttggggtgcg cgcgatttac tgggagataa ataatggcgc
     9901 tgcatgacga aaacgtcgtc tggcatagcc acccagtaac tccgcaacaa cgcgaacagc
     9961 accacggtca tcgtggtgtt gtgctgtggt ttaccggcct ctctggttcc ggtaaatcaa
    10021 ccgtcgcggg ggcgctggag gaggcgttac ataaacttgg cgtcagtacg tatctgctgg
    10081 atggcgacaa cgttcgccac ggattatgca gcgatctcgg ttttagcgat gccgaccgta
    10141 aagagaatat ccgtcgggtt ggtgaagtgg cgaatttgat ggttgaagcc ggactggtgg
    10201 tgctgaccgc atttatctcg ccacaccgcg ccgaacgcca gatggttcgc gaacgcgtag
    10261 gagaagggcg ctttatcgaa gtgtttgtcg atacgccgct ggcgatttgc gaagcccgcg
    10321 atccaaaagg tttatataag aaagcgcgtg ccggtgaact gcgcaacttt acgggaatag
    10381 attccgttta cgaagcgcct gaatcggcag aaattcatct caatggtgaa caattagtaa
    10441 caaatttggt acagcaatta ttagatctgt tgagacagaa cgatattatc agatcctgag
    10501 acaccgccgg gtttccatgc ccggttcgtc aaagtaacag gatcagatat gcgtaacagc
    10561 cacaacatta cattaacaaa taacgacagc cttactgagg ttgaagacac cacatggtcg
    10621 ctgccaggcg ctgtggttgg ttttttatcg tggctgtgcg cgttggcaat tccgatgttg
    10681 atttatggct ctaacacgct gctctttttt atctacacct ggcctttctt tctggcgctg
    10741 atgcccgtcg ctgtagtggt ggggattgca ctgcattcat tgatggacgg aaagctacgc
    10801 tacagtattg ttttcactct ggcgacggtt ggcattatgt ttggtgcgct gtttatgtgg
    10861 ctactgggtt aattgttctt tcctctctat tctgttcata attcaatccg taactaataa
    10921 tgagattatg ttctgcacgc cctgggtata cgtaacaatg gacaaatgtg gtacatttgc
    10981 ccgcgttgtc gcggtatccc caacagagga tgtagagtcg tcttcggatg catgggatga
    11041 agatgccgtt tttcaggggg caggatgggt aaactaacgc tgctgttgct ggctattctg
    11101 gtctggctac agtattcgct gtggttcggt aagaacggta tacatgacta tactcgcgtc
    11161 aataatgatg tggcggcaca gcaagctaca aacgcgaaac ttaaagcgcg aaacgatcag
    11221 ctttttgccg aaattgacga tctcaatggc ggccaggagg cgctcgaaga gcgtgcgcgt
    11281 aatgaactca gcatgaccag gccgggcgaa acgttttatc gtctggtgcc tgacgcgtca
    11341 aagcgcgcac agtctgcggg gcaaaataat cgataaatca gcccgggaat taacatggca
    11401 accactcatt tggatgtttg cgccgtggtt ccggcggccg gatttggccg ccgaatgcaa
    11461 acggaatgtc ctaagcaata tctctcaatc ggtaatcaaa ccattcttga acactcggtg
    11521 tatgcgctgc tggcgcatcc cagggtgaaa cgtgtcgtca ttgccataag tcctggcgat
    11581 agccgctttg cacaacttcc tctggcgaat catccacgaa tcacggttgt tgatggtggt
    11641 gaagagcgtg ccgattccgt gctggcaggt ctgaaagccg ctggcgacgc gcagtgggta
    11701 ttggtgcatg acgccgctcg tccttgtctg catcaggatg acctcgcgcg attgttggcg
    11761 ttgagcgaaa ccagccgcac gggggggatc ctcgctgcgc cggtgcgcga tacgatgaaa
    11821 cgtgccgaac cgggcaaaaa tgccattgct cataccgttg atcgcaacgg cttatggcac
    11881 gcgctgacgc cgcaattttt ccctcgtgag ctgttacatg actgcctgac gcgcgctcta
    11941 aatgaaggcg ctaccattac cgatgaggcc tcggcgctgg aatattgcgg ttttcatccg
    12001 caacttgttg aaggccgcgc ggataacatc aaggtaacgc gcccggagga tttggcgctg
    12061 gccgaatttt acctcacccg aaccatccat caggagaata cataatgcga attggacacg
    12121 gttttgacgt acacgccttt ggcggtgaag gcccaattat cattggtggc gtacgcattc
    12181 cttacgaaaa agggctgctg gcgcattctg atggcgacgt ggcgctccat gcgttgaccg
    12241 atgcattgct tggcgcggcg gcgctgggag atataggcaa gctgttcccg gataccgatc
    12301 cggcatttaa aggtgccgac agccgcgagc tgttgcgcga agcctggcgt cgtattcagg
    12361 cgaaaggtta tgccctcggc aacgtcgatg tcactatcat cgctcaggca ccgaagatgt
    12421 tgccgcacat tccacaaatg cgcgtgttta ttgccgaaga tctcggctgc catatggatg
    12481 atgttaacgt gaaagccact actacggaaa aactgggatt taccggacgt ggggaaggga
    12541 ttgcctgtga agcggtggcg ctactcatta aggcaacaaa atgattgagt ttgataatct
    12601 cacttacctc cacggtaaac cgcaaggcac cgggctgctg aaagccaatc cggaagactt
    12661 tgtggtggtg gaagatttgg gctttgagcc tgatggtgaa ggtgagcata ttctggttag
    12721 aatcctcaaa aacggctgca atacccgttt tgtggcggat gcactggcga aattcctgaa
    12781 aattcatgcc cgtgaagtca gcttcgctgg gcaaaaagat aaacatgctg ttacggaaca
    12841 gtggttatgc gctcgcgtgc cgggcaaaga gatgcccgat ctgagcgcct ttcaactgga
    12901 aggctgccag gtgctggagt atgcgcggca caagcgcaag ctgcgtttag gggcgctgaa
    12961 aggtaacgcc tttaccctgg tactacgcga agtgagcaat cgcgatgacg ttgaacaacg
    13021 tctgatcgat atttgcgtaa aaggtgtacc gaactacttc ggtgcccaac gttttgggat
    13081 tggcggtagc aacttgcagg gcgcgctgcg ttgggcgcaa accaatactc cggtgcgcga
    13141 tcgcaataaa cggagttttt ggttgtcggc agcccgcagt gcgttgttta atcagattgt
    13201 tgctgagcgc ctcaaaaaag cagacgttaa tcaagttgtt gacggcgatg cgctacaatt
    13261 agccggacgt ggtagctggt ttgttgcaac caccgaagaa ctggcggaat tacagcgtcg
    13321 cgtcaacgat aaagagctga tgataaccgc cgcattgccg ggcagtggcg aatggggaac
    13381 tcagcgtgaa gcgctggcat tcgaacaagc agctgtcgcc gaagaaactg aattacaaac
    13441 tttactggtg cgtgaaaaag ttgaagccgc gcgcagagcg atgctgctgt atccgcaaaa
    13501 attaagctgg aactggtggg atgacgtcac cgtagaaatc cgtttctggc ttccggcggg
    13561 aagttttgca accagcgttg tcagggaact tatcaacaca acaggtgatt atgcgcatat
    13621 tgctgagtaa tgatgacggg gtacatgcac ccggtataca aacgctggcg aaagccttgc
    13681 gtgagtttgc tgacgttcag gtggtcgccc ccgatcgtaa ccgcagcggc gcttcaaatt
    13741 ctctgacact ggaatcctcc ctgcgcacgt ttacctttga aaatggtgat attgctgtgc
    13801 aaatgggaac cccgaccgat tgcgtctatc ttggcgtgaa tgctctgatg cgtccgcgcc
    13861 cggacattgt tgtgtccgga attaacgccg ggccgaatct gggggatgat gttatttatt
    13921 ccggtacggt agccgccgcg atggaaggcc gtcatttagg ttttccggcg ctggccgtct
    13981 cgcttgacgg gcataaacat tacgacactg ccgcggcggt aacctgttca attttgcgcg
    14041 cactgtgtaa agagccgctg cgcaccggac gtattcttaa tattaacgtt ccggatttac
    14101 cattggatca aatcaaaggt attcgcgtga cgcgctgcgg tacacgacat ccggcagatc
    14161 aggtgatccc gcagcaagat ccgcgcggca atacgctgta ctggattggc ccgccgggcg
    14221 gtaaatgtga tgccggtccg gggaccgatt ttgctgcggt agatgagggc tatgtttcca
    14281 tcacgccgct gcatgtggat ttaactgcgc atagcgcgca agatgtggtt tcagactggt
    14341 taaacagcgt gggagttggc acgcaatggt aagcagacgc gtacaagcac ttctggatca
    14401 attacgtgcg caaggtattc aggatgagct ggtgctgaat gcacttgccg ccgtgccgcg
    14461 tgaaaaattc gttgatgaag cgtttgaaca aaaagcctgg gacaatatcg ccttgccgat
    14521 aggtcagggg cagacaattt cgcagccgta tatggtggcg cgaatgaccg aattactcga
    14581 actgacgccg cagtcgcggg tgctggaaat tggcaccggt tcgggatatc aaacggcaat
    14641 cctggcgcat cttgtccagc atgtttgctc ggttgaacgg attaaaggct tgcagtggca
    14701 ggcgcgtcgc cgcctgaaaa atcttgattt acataatgtt tcaacccgtc atggcgatgg
    14761 atggcaaggt tggcaggcac gtgcgccgtt tgacgctatc attgttacgg cggcaccgcc
    14821 ggaaattcca actgcgctaa tgacgcagct ggacgaaggc ggaattctcg tcttacccgt
    14881 aggggaggag caccagtatt tgaaacgggt gcgtcgtcgg ggaggcgaat ttattatcga
    14941 taccgtggag gccgtgcgct ttgtcccttt agtgaagggt gagctggctt aaaacgtgag
    15001 gaaatacctg gatttttcct ggttattttg ccgcaggtca gcgtatcgtg aacatctttt
    15061 ccagtgttca gtatggtgcc ttgcacggta attatgtcac tggttattaa ccaatttttc
    15121 ctgggggata aatgagcgcg ggaagcccaa aattcaccgt tcgccgcatt gcggctttgt
    15181 cactggtttc gctatggctg gcaggctgtt ctgacacttc aaatccaccg gcaccggtca
    15241 gctccgttaa tggcaatgcg cctgcaaata ccaattctgg tatgttgatt acgccgccgc
    15301 cgaaaatggg gacgacgtct acagcgcagc aaccgcaaat tcagccggta cagcagccac
    15361 aaattcaggc tactcaacaa ccgcaaatcc agccagtaca gccagtagct cagcagccgg
    15421 tacagatgga aaacggacgc atcgtctaca accgtcagta tgggaacatt ccgaaaggca
    15481 gttatagcgg cagtacctat accgtgaaaa aaggcgacac acttttctat atcgcctgga
    15541 ttactggcaa cgatttccgt gaccttgctc agcgcaacaa tattcaggca ccatacgcgc
    15601 tgaacgtcgg tcagacctta caggtgggta atgcttccgg tacgccaatc actggcggaa
    15661 atgccattac ccaggccgac gcagcagagc aaggagttgt gatcaagcct gcacaaaatt
    15721 ccaccgttgc tgttgcgtcg caaccgacaa ttacgtattc tgagtcttcg ggtgaacaga
    15781 gtgctaacaa aatgttgccg aacaacaagc caactgcgac cacggtcaca gcgcctgtaa
    15841 cggtaccaac agcaagcaca accgagccga ctgtcagcag tacatcaacc agtacgccta
    15901 tctccacctg gcgctggccg actgagggca aagtgatcga aacctttggc gcttctgagg
    15961 ggggcaacaa ggggattgat atcgcaggca gcaaaggaca ggcaattatc gcgaccgcag
    16021 atggccgcgt tgtttatgcc ggtaacgcgc tgcgcggcta cggtaatctg attatcatca
    16081 aacataatga tgattacctg agtgcctacg cccataacga cacaatgctg gtccgggaac
    16141 aacaagaagt gaaggcgggg caaaaaatag caaccatggg tagcaccgga accagttcaa
    16201 cacgcttgca ttttgaaatt cgttacaagg ggaaatccgt aaacccgctg cgttatttgc
    16261 cgcagcgata aatcggcgga accaggcttt tgcttgaatg ttccgtcaag ggatcacggg
    16321 taggagccac cttatgagtc agaatacgct gaaagttcat gatttaaatg aagatgcgga
    16381 atttgatgag aacggagttg aggtttttga cgaaaaggcc ttagtagaag aggaacccag
    16441 tgataacgat ttggccgaag aggaactgtt atcgcaggga gccacacagc gtgtgctgga
    16501 cgcgactcag ctttaccttg gtgagattgg ttattcacca ctgttaacgg ccgaagaaga
    16561 agtttatttt gcgcgtcgcg cactgcgtgg agatgttgcc tcccgccgcc ggatgatcga
    16621 gagtaacttg cgtctggtgg taaaaattgc ccgccgttat ggcaatcgtg gtctggcgtt
    16681 gctggacctg atcgaagagg gcaacctggg gctgatccgc gcggtagaga agtttgaccc
    16741 ggaacgtggt ttccgcttct caacatacgc aacctggtgg attcgccaga cgatcgaacg
    16801 ggcgattatg aaccaaaccc gtactattcg tttgcctatt cacatcgtaa aggagctgaa
    16861 cgtttacctg cgaaccgcac gcgagttgtc ccataaactg gaccacgaac caagtgcgga
    16921 agagatcgca gagcaactgg ataagccagt tgatgacgtc agccgtatgc ttcgtcttaa
    16981 cgagcgcatt acctcggtag acaccccgct gggtggtgat tccgaaaaag cgttgctgga
    17041 catcctggcc gatgaaaaag agaacggtcc ggaagatacc acgcaagatg acgatatgaa
    17101 gcagagcatc gtcaaatggc tgttcgagct gaacgccaaa cagcgtgaag tactggcacg
    17161 tcgattcggt ttgctggggt acgaagcggc aacactggaa gatgtaggtc gtgaaattgg
    17221 cctcacccgt gaacgtgttc gccagattca ggttgaaggc ctgcgccgtt tgcgcgaaat
    17281 cctgcaaacg caggggctga atatcgaagc gctgttccgc gaataagtaa gcatctgtca
    17341 gaaaggccag tctcaagcga ggctggcctt tttcttttgt ttgggaagtc ggtgtgtttt
    17401 gtcgcctgga taagactcgt cagcgtccta cccggcaatc agcatcctat ttgctcatca
    17461 tcttgcgcac caactgcata aacagctcct gctcttctgc actcaaacgc cccaaaaatt
    17521 cctcatccac actgtcacca atggggatcg ccgcagcaag taccttttct ccttcggcag
    17581 tcagccagac aaagcgtcgc cgcttgtcag cggcgtcatg ttcacgtctg actaagccac
    17641 gattttccat tcttgccaac atttctgcca gcgttgcttt ggtgctgact gctgcttcta
    17701 tcagtgccac ctgttcgata ccaggcttat cagcaatggc gcgcatcacc gcgtactgtg
    17761 gtttggtcag gtcaggtaac tcgtgctgcc agcgagctgt atgctgctga aaaagctgtc
    17821 gtaactgatg gaacgcttta tttcgtaacg ccatgtaaac tccgggctaa ttctctgtcg
    17881 ctatcatagc ggtttttcag tgacgaagaa gatgttgtta cttttcaatc cttgccgctg
    17941 tggaaatgaa ggagtatgtt aataataacg ttcgtatacg aacaattaag agagtaagca
    18001 atgaaactga tcgtcgggat gacaggggct accggtgcgc ctcttggtgt ggcattactg
    18061 caagcgctgc gggagatgcc gaatgtcgag actcatctag tgatgtcgaa gtgggcgaaa
    18121 accacgattg aactggaaac gccttacagc gctcgcgatg ttgccgccct cgcagacttc
    18181 agccataacc cggcggatca ggcggcgact atctcttccg gctcctttcg taccgacggc
    18241 atgatcgtta ttccatgcag tatgaaaacg ctcgccggta tccgcgctgg ttacgccgat
    18301 ggcctggtgg ggcgcgcggc ggacgtcgtg ctcaaagaag gccgcaaact ggtgctggtg
    18361 ccgcgtgaaa tgccgcttag cacaatccat ctcgaaaata tgctcgcact ttcgcgcatg
    18421 ggcgtggcga tggtgccgcc gatgcctgcc ttttataacc atcccgaaac ggtagatgac
    18481 attgtccacc acgtggtagc ccgcgtgctg gatcaatttg gcctcgaaca tcctcacgcc
    18541 aggcgctggc aaggattgcc gcaggcccgg aatttttctc aggagaatga ataatggcat
    18601 ttgatgattt acgcagcttt ttacaggcgc ttgatgacca cggccagtta ctgaaaatca
    18661 gcgaagaggt gaacgcggaa ccggatctgg cagccgcagc caacgccacc gggcgtatcg
    18721 gcgacggcgc gcccgcgctt tggtttgata atattcgcgg ctttatcgat gcccgcgtgg
    18781 caatgaacac catcggttcc tggcagaacc acgcgatttc cctcggcctg ccgccaaaca
    18841 ccccggttaa aaaacagatt gatgagttta tccgccgctg ggataacttc cccattgccc
    18901 cggagcgccg cgccaatcca gcctgggcgc agaacaccgt cgatggcgac gagatcaacc
    18961 tgttcaatat tctgccgctg tttcgtttaa acgacggtga tggcggtttc tatctcgata
    19021 aagcgtgcgt ggtttcccgc gatccgctcg acccggataa cttcggcaag cagaacgtcg
    19081 gcatctaccg catggaagtg aagggcaagc gtaagctcgg cctgcaaccg gtgccgatgc
    19141 acgatatcgc cctgcatctg cataaagcag aagagcgcgg tgaagatctg ccgattgcca
    19201 tcacactcgg taacgatccg atcatcaccc taatgggggc cacgccgctg aaatacgatc
    19261 agtctgagta cgaaatggca ggcgcgctgc gcgaaagccc gtacccgatc gccaccgccc
    19321 cgctgaccgg ttttgatgtg ccgtggggct cggaagtgat cctcgaaggg gttatcgaaa
    19381 gtcgtaaacg tgagattgaa gggccgttcg gtgagtttac cggccactac tccggcgggc
    19441 gtaacatgac cgtggtgcgt atcgataaag tctcttaccg taccaaaccg attttcgaat
    19501 cgctctatct tggtatgccg tggaccgaaa tcgactacct gatggggcct gccacctgcg
    19561 tgccgctgta tcaacaactg aaagccgagt tcccggaagt gcaggcggta aacgccatgt
    19621 acacccacgg cctgctggcg attatctcca ccaaaaaacg ctacggcggc tttgcccgcg
    19681 cggtgggcct gcgtgcgatg accacgccgc acggtctggg ctacgtgaag atggtgatta
    19741 tggtcgatga agacgttgat ccgttcaacc tgccgcaggt gatgtgggcg ctctcctcga
    19801 aagtgaaccc ggcaggggat ttggtacagt tgccgaatat gtccgtgctg gaactcgacc
    19861 ctggctcaag tccggcgggg atcaccgaca agctgattat cgacgccacc acgcctgtcg
    19921 ccccggacaa ccgtggtcac tacagccagc cggtggtgga cttaccggaa accaaagcct
    19981 gggctgaaaa actgaccgct atgctggccg cacgtcaata aggagaacaa gatgatttgt
    20041 ccacgttgtg ccgatgaaca gattgaagtg atggcgaaat cgccggtaaa agatgtctgg
    20101 atggtctacc agtgccagca ttgcctctat acctggcgcg ataccgaacc gctacgccgc
    20161 accagccgcg aacattatcc cgaagcgttc cgcatgacgc agaaagatat tgatgacgcg
    20221 ccgatggtgc cgagcatccc gccgctgctg gcggaaggta agcgctaaat aaaaggtccg
    20281 atgcccatcg gaccttttat taaggtcaaa ttaccgccca tatgcaccag gtaattaaga
    20341 atccggtaaa accgagaatg gtcgttaaca ctgtccaggt tttcagcccg tctgctaccg
    20401 acaaccccag atatttggtc acaatccaga accctgagtc attaatatgt gacgcgccaa
    20461 gcccaccaaa gcaggctgcc agcgtcacca atacgcactg aattggattc aatcccatca
    20521 ccgcttctga gagtaacccg ccggttgtca ggattgctac ggttgctgac ccctgcgatg
    20581 cacgcagcgc cagtgaaata ataaatgcgg ctggtaacag aggcaggtca atcatttgta
    20641 acatgttggc aagggcttta ccgacgcccg attccaccag cactttgcca aatacccctc
    20701 cagcaccagt aaccaaaatc actaccgccg cagtgggaag ggctgagccc ataatgtcgc
    20761 tggtgtgttg taagctccag ccgcgacgta aagccaataa ccagaatgcc agcaccagcg
    20821 caatcattag agctaccatt ggtgagccga tcagctgtag cgtaccaagc aggggatgcg
    20881 aaggcggcat cagtgttgcg gaaaccgtac ccgccatgat aatcgcgata ggaataacaa
    20941 ttagcgaggt gaccagcgcg acgcccggtg gatttatttt atcgcttaat tttgtcgcgc
    21001 cttcctcact ggccggagcc agttgcatct gttccagtac ctcgaccgac atcgcatatt
    21061 ggcgcttatt gattattttc gctgcaaagt agccaacaat ccctacggga atagaaatcg
    21121 caataccgat gatggttagc cagccgatgt ctgcgtggag taaccccgct gcggcgacag
    21181 ggcctggatg aggcggtacc gccacgtgaa cagtgagcat gatcccagcg acaggcagac
    21241 caaatttgag tggtgatatt ttggcaacct tggcaaaacc gtaaatgatt ggcgcaagaa
    21301 taataaagcc gacatcaaag aagacgggaa taccgaggaa gaacgctgcc agagtcagcg
    21361 cagcgatagt tcgtttgtcg cctaacttgc gactgaaata attagccagt gactctgcac
    21421 caccagagtg ttcgatcata cgccccagca tagcgcccag accaataata atagtgacgg
    21481 aaccaagcac accgcccatc ccggcgatca tcactttacc cacttcgccc gccggtatac
    21541 ccgccgcaag tgcgactaac aggctgacga ggagcagagc aacgaatggt tgtacctttg
    21601 ccttgatgac cagcagcaac agcatgatta cgccagttaa cgcaatgcat aacaatgtaa
    21661 ttgtggacat gggaaaccct gtctgaaagt tatagttaac ctaccccatc cgtagatggg
    21721 gggatgtatg ggtacgttgt aattagggat ttaacgaatt agcgccaggc gtcaaaccag
    21781 ccaagtcctt cttcggtgag gccacgaggt ttgtattcac aaccgatcca gccctgatat
    21841 cccacttcat cgaacaggcg gaacagccac ggatagttga tttctccatc gtccggttca
    21901 tgtcgatcag gtagtccggc aatttgtacg tgcgcatatt tcccggcgta gtcgcggatt
    21961 aaatgcgtca ggttgccatc tactttttgc gcatggaaag tatctagttg aataaaaacg
    22021 ttatctcgcg caacctcttc aacaatagcc agtgcctgat actggctgga gaagagataa
    22081 tgaggcttaa cgccggggct gagtgcttca accaatattc gcttgccgtg tggcgcaaag
    22141 tggtcggcag cgtagcggag attatcaata aatactgccc ggtaccgttc agcatcttcg
    22201 ccagcgggca cgacgcctgc catcacatgg acttgttcac aattgagcgc caatgcatat
    22261 tccagtgcca ggtcgatgtc tgcacgtgct tcgtgctcac gtccgggaag ggcggataat
    22321 ccccattctc ccgcattaat atctccggga gcggtattga acagcgccag cgtcaggtgg
    22381 ttttgcgcca gttgctgctg gatttgctgg ggggagtagt catagggaaa cagaaactcc
    22441 acagcatcga acccggcttt tcgcgctgcg gcgaagcgtt caataaaagg cacttcggtg
    22501 aacatcatgg ataaattagc tgcaaaacga ggcattgcat taactcctta attccgcaat
    22561 ttcacctgcg gtcagataac ggatcgggcg gtcaccgaga ataaaaatca gctttgccgt
    22621 ttcctctagc tcttccatat tgttggcggc ttcttgcagg ctttcaccgc aaaccactgg
    22681 gccatgattt gccagtaaaa aagcctgatt gtctgctgcc agttccgcca gatcctgtgc
    22741 gatgcgttta tcgcccggtc ggtaataggg caccagcggg acgtttccca tccgcatcac
    22801 cacgtatggt gtgaacggcc gaataacgtt attgctgtcc agcccttcca ggcaggaaag
    22861 cgccgtcgac cacgtactgt gcaaatgcac taccgcttta cagcgcggat tgttgcgata
    22921 cagcgccaga tgaaagagca cctctttcga gggtttgtca ccacttaacc attcgccatc
    22981 cgcggtgact ttggaaagcc gctgcggatc gagattgccc aggcatgaac ctgtcggtgt
    23041 cgccagtaaa ttcccgtcag gtaaaagcag cgacagattg ccagccgaac cagttgcata
    23101 tccgcgctga aagaatgaac tggcaatccg cgtcatctcc tctcgcaaag actgctctac
    23161 ttttgcgaaa tcgctcatga taaaaactct ctttgggctc gtgaaaaaaa ggcttcatca
    23221 ccgaagttgc cagatttaag ggcgagtgag acaggcttat ccagtgcgtt tacccacggc
    23281 acgccggggg aaatggttgg gccaatatga aaacctttta tgcccaggct ctgtgtgact
    23341 acgccggagg tctcaccgcc tgcgacaata aagcgtgtca cgccttccgc tgctaaccgc
    23401 gccgctagtt gagaaaacag tgtttccact gcctgactgg ctttttgtgc accgtattgc
    23461 tgttgaattg ctgccaatgc gtcagtgctg gcggtggcaa aaaccagtgg agcaagtaca
    23521 ctttcctggc ccagaaccca ctctgccagc tcgtgtgcat aagcggccag agtttcggtt
    23581 gagaggcagc gtgccacatc aacctcacgg gctggtgcaa tttgacggta atgtgccacc
    23641 tggcggttgg tcatttgaga gcatgaaccg gagagcacta cgccgcgccc agcgagcgga
    23701 cgccctgctt cgcgagcctg gttaccgttt tcttgcgccc actgccgggc aaggccaatc
    23761 gccagaccag aaccgcccgt taccagtggg gtatcgcgca aggcttctcc ctgaatttcc
    23821 agatggtgtt cggtcagcgc atcaagcacc gcgtagcggt agccctcttg ctgtaagcga
    23881 gccagctcct gacgaacggc atccacacct tgttcgaaaa catgtgccga aacgacgccg
    23941 cagcgccctg tggattgcgc ttcaaccaga cggggaagat agctgtcggt catgggattt
    24001 accgggtgat ggcgcatccc ggattcggcc agcagttgat tcattacgaa caaatacccc
    24061 tgataaaccg tacgtccgtt gaccggcagg gccggagaga agaccgtaaa cggcgtgtcg
    24121 agagcatcca ttaatgcatc ggtaaccggg ccgatattac ctttcgccga actgtcgaaa
    24181 gtagagcagt atttgaaata gatctgtttg caaccttgct gttgcaacca gctcagagcc
    24241 gccagcgatt gctgtgtggc ttcaaccacc ggacaggagc gcgttttcag gctgatcacc
    24301 agtgcgtcga ttgcttccgg cattttacct gttggaacac cgttaatttg taccgttggt
    24361 agaccgtttt ccaccagaaa actggcgata tccgtcgcgc cggtaaaatc atcggcgata
    24421 acgccaatct tgatcatgat ttcgctcccg gtagggtgat gccagagaaa atcttgataa
    24481 ctgcgctatc gtcttctttc ccgtaacccg cgttactggc gctggtgaac atattcaatg
    24541 ctgttgaggc caatggcagc gggaagtgca gggctttggc ggtatcggca accagaccaa
    24601 gatccttaac aaaaatatcg acggctgaat gcggggtgta atcgccatcc accacatgac
    24661 gcatccggtt ttcgaacatc catgaatttc cggcggcatt ggtcacgacg tcatacatca
    24721 catccagcgg gatccccgca cgggctgcaa gtgccatcgc ttcggctccg gcagcaatat
    24781 gtacgcccgc taacaactgg tgaataattt ttacggtcga acctagtccc ggttctgcac
    24841 ctatgcgata aacttttccg gcaacggctt ccagcacggg tgctagtcgt tcaaaggcaa
    24901 tatcgctacc ggaggccatg acagtcattt caccgttagc ggcttttact gcaccaccag
    24961 aaactggcgc atccagcatt tccagatcga atccagccag agcggtagca atttcttgcg
    25021 catcagcact ggcgatagtg gaagaaacca ttactgccgt accgggtttc agatgttgtg
    25081 caacgcctgt ttcaccaaac agcacctgtt taacctgggc cgcattgacc accagcacca
    25141 gcagagcgtc cagtttttcg gcaaacgtcg cggcgttatc agaaactccg caagcacctg
    25201 cctctttcaa cgtagcgcag gcattgctgt tcaggtctgc gccccaggta gaaagacctg
    25261 cgcggacaca tgacagtgct gctcccattc ccattgaccc taagccaacg ataccgacat
    25321 gaaactccga tcccgttttc atctgctctc cttgttaatt taagtgatat tttgtttgat
    25381 gttgtgaata taagcgcgga aagataacga tatggtgagc tgattcacaa aaattaacat
    25441 tgtatgttat tttatgtgaa ctaagcgtta gttgcgccgc gcacgtttcg caggcaaata
    25501 gcgtagaatg tcagcaggac aaaggaagga gcaaaagttg atacccgtag agcgtcgcca
    25561 aatcatcctt gagatggtag ctgaaaaagg cattgttagt attgctgaac taacggacag
    25621 aatgaatgtg tcacatatga ccattcgtcg ggatttacaa aaactggagc agcagggagc
    25681 cgttgtgttg gtgtccgggg gcgtccagtc tccgggacgg gtggcgcatg aaccttctca
    25741 tcaggtaaaa actgcgctgg caatgacgca aaaagcggct attggcaagc tggcggcaag
    25801 tcttgttcag ccgggacgtt gtatctatct ggatgcggga acgaccacgt tagcgattgc
    25861 acagcatctg actcacatgg agccactcac agtggttact aacgacttcg ttattgcgga
    25921 ctacttgctc gacaacagta attgcacaat tattcacact ggcggcgcag tgtgtcggga
    25981 aaaccgttcc tgtgttgggg aagccgctgc gaccatgctg cgtagcctga tgattgatca
    26041 ggcttttatt tctgcatcgt catggagtgt gcgggggatc tctacgccag cagaagataa
    26101 agtcacggtg aaacgggcga ttgccagtgc cagccgccag cgagttttgg tctgtgatgc
    26161 gacgaaatat ggacaggtgg cgacatggct ggcgttacca ttaagcgagt ttgatcagat
    26221 cattactgac gatggtctgc cggagagtgc cagccgcgcg ctggcgaagc tggatctctc
    26281 tttgctggta gcgaaaaatg aataatgacc tgcaataacg ccgggttact catgcttcac
    26341 agaagaagca tgagactact ttattttata aaatgacagc cgcccgcttt tcggcgatcc
    26401 ggtatcaata taaatctggt ttgcgaacgt ctgaatgtta tcaaacatca tatgtccaaa
    26461 tataaaataa tcagcgccgt ttattttttg taattcgcta ttaagtgatt tctgcacacg
    26521 atcaacaggc cagagtaatt cgctctccgc tatttctttg ccaaactgat actccttccc
    26581 cggataatct gcatgtgcga tgacatattt tatggtgtcg ttagtgattt caataatatg
    26641 tggaaggtga tggaatttca gcaacagatc tattgcctct tgttgctctg aatcatttaa
    26701 atcgaaaaac cagtcaccac cgctggcaag ccacatattt ccatcgccag tttcgaatgc
    26761 ctcaagcgcc atcgcttcgt ggttgccttt gacggagata aaccagggct ggtttaacag
    26821 gcgcagcacg ttaagactct cgggcccacg atcgatgtta tcgccgacag aaataagcaa
    26881 gtcggtttca ggacaaaaag agagttgatg taagcgggat tgtaataatt gatagtcacc
    26941 atgaatatca ccaacggccc atatatggtg atagtgatgg gcattgattt tttggtagcg
    27001 tgtagatggc atggttttac c
//
LOCUS       DAZGAP010000058        27003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGAP010000058 DAZGAP010000000
VERSION     DAZGAP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.058"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            244..2667
                     /gene="pgaA"
                     /locus_tag="ACHLAM_002034"
     CDS             244..2667
                     /gene="pgaA"
                     /locus_tag="ACHLAM_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO7853561.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLVELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2676..4694
                     /gene="pgaB"
                     /locus_tag="ACHLAM_002035"
     CDS             2676..4694
                     /gene="pgaB"
                     /locus_tag="ACHLAM_002035"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO7853562.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4687..6012
                     /gene="pgaC"
                     /locus_tag="ACHLAM_002036"
     CDS             4687..6012
                     /gene="pgaC"
                     /locus_tag="ACHLAM_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO7853563.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6014..6427
                     /gene="pgaD"
                     /locus_tag="ACHLAM_002037"
     CDS             6014..6427
                     /gene="pgaD"
                     /locus_tag="ACHLAM_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO7853564.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6477..7265)
                     /gene="phoH"
                     /locus_tag="ACHLAM_002038"
     CDS             complement(6477..7265)
                     /gene="phoH"
                     /locus_tag="ACHLAM_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO7853565.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7891..9162)
                     /gene="efeB"
                     /locus_tag="ACHLAM_002039"
     CDS             complement(7891..9162)
                     /gene="efeB"
                     /locus_tag="ACHLAM_002039"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO7853566.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGVISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9168..10295)
                     /gene="efeO"
                     /locus_tag="ACHLAM_002040"
     CDS             complement(9168..10295)
                     /gene="efeO"
                     /locus_tag="ACHLAM_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO7853567.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10353..11183)
                     /gene="efeU"
                     /locus_tag="ACHLAM_002041"
     CDS             complement(10353..11183)
                     /gene="efeU"
                     /locus_tag="ACHLAM_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO7853568.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSVFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11849..13357)
                     /gene="putP"
                     /locus_tag="ACHLAM_002042"
     CDS             complement(11849..13357)
                     /gene="putP"
                     /locus_tag="ACHLAM_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO7853569.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(13516..13725)
                     /locus_tag="ACHLAM_002043"
     CDS             complement(13516..13725)
                     /locus_tag="ACHLAM_002043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853570.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13780..17742
                     /gene="putA"
                     /locus_tag="ACHLAM_002044"
     CDS             13780..17742
                     /gene="putA"
                     /locus_tag="ACHLAM_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7853571.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPPVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17782..18420)
                     /gene="rutR"
                     /locus_tag="ACHLAM_002045"
     CDS             complement(17782..18420)
                     /gene="rutR"
                     /locus_tag="ACHLAM_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO7853572.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18708..19799
                     /gene="rutA"
                     /locus_tag="ACHLAM_002046"
     CDS             18708..19799
                     /gene="rutA"
                     /locus_tag="ACHLAM_002046"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO7853573.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDSLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAAHAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSDTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            19799..20491
                     /gene="rutB"
                     /locus_tag="ACHLAM_002047"
     CDS             19799..20491
                     /gene="rutB"
                     /locus_tag="ACHLAM_002047"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO7853574.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            20503..20889
                     /gene="rutC"
                     /locus_tag="ACHLAM_002048"
     CDS             20503..20889
                     /gene="rutC"
                     /locus_tag="ACHLAM_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO7853575.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20897..21697
                     /gene="rutD"
                     /locus_tag="ACHLAM_002049"
     CDS             20897..21697
                     /gene="rutD"
                     /locus_tag="ACHLAM_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO7853576.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21707..22297
                     /gene="rutE"
                     /locus_tag="ACHLAM_002050"
     CDS             21707..22297
                     /gene="rutE"
                     /locus_tag="ACHLAM_002050"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO7853577.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22308..22802
                     /gene="rutF"
                     /locus_tag="ACHLAM_002051"
     CDS             22308..22802
                     /gene="rutF"
                     /locus_tag="ACHLAM_002051"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO7853578.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22823..24151
                     /gene="rutG"
                     /locus_tag="ACHLAM_002052"
     CDS             22823..24151
                     /gene="rutG"
                     /locus_tag="ACHLAM_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO7853579.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24234..24407)
                     /gene="ymdF"
                     /locus_tag="ACHLAM_002053"
     CDS             complement(24234..24407)
                     /gene="ymdF"
                     /locus_tag="ACHLAM_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7853580.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24780..25376
                     /gene="wrbA"
                     /locus_tag="ACHLAM_002054"
     CDS             24780..25376
                     /gene="wrbA"
                     /locus_tag="ACHLAM_002054"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7853581.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNS"
     gene            25397..25624
                     /gene="yccJ"
                     /locus_tag="ACHLAM_002055"
     CDS             25397..25624
                     /gene="yccJ"
                     /locus_tag="ACHLAM_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO7853582.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25662..26903)
                     /gene="agp"
                     /locus_tag="ACHLAM_002056"
     CDS             complement(25662..26903)
                     /gene="agp"
                     /locus_tag="ACHLAM_002056"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO7853583.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
BASE COUNT         6568 a         6998 c         6866 g         6571 t
ORIGIN      
        1 ttttgaaaaa ggcattggga tttatgccgt attcctgaag atcctcatca ttggaatgga
       61 ttttcgggcg agaaaaggat tttatatgga caatctgctc atcatttctt cttctcatca
      121 tcaacaattc acgtgtctct tccgcgttta ataacggatt atgagatgca aaaatatctt
      181 tcttttcagt tacctgtaat tagatataga gagagatttt ggcaatacat ggagtaatac
      241 aggatgtatt caagtagcag aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt
      301 actgccgcat ttttagcagc gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat
      361 gcattgatta ttgaagctcg caaaggaaat actcagccag ctttgtcatg gtttgcacaa
      421 aaatcaacac tcagtaataa ccaaattgct gactggttac aaattgcctt atgggccggg
      481 caagataaac aggtcattac cgtttacagc cgctaccgtc atcagcaatt accagcgcgt
      541 ggttatgcag ctgtcgccgt cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca
      601 ctgtggcaaa aggcgctctc tctggagccg caaaataagg attatcaacg gggacaaatt
      661 ttaaccctgg cagatgcggg tcactatgat actgcgctgg ttaaacttaa gcagcttaaa
      721 tctggagcgc cggacaaagc caatttactc gcagaagcct atatctataa actgacgggg
      781 cgtcatcagg atgaattacg ggcgatgaca gagtcattac ctgaaaatgc atctaagcaa
      841 caatatccca cagaatacgt gcaggcatta cgtaataatc aactagctgc cgcgattgac
      901 gatgccaatt taacgccaga tattcgcgct gatattcatg ccgaactgat cagactgtcg
      961 tttatgccta cgcgcagtga aagtgaacgt tatgccattg ccgatcgcgc cctcgcccaa
     1021 tacgctgcat tagaaattct gtggcacgat aactcagacc gcactgccca gtaccagcgt
     1081 attcaggttg atcatcttgg cgcgttatta actcgcgatc gttataaaga cgttatttct
     1141 cactatcagc gtttaaaaaa tacgaggcaa attattccgc cctgggcgca atattgggtt
     1201 gcgtcggctt atctcaaaga acagcagcca aaaaaagcac agtcaataat gacagagctc
     1261 ttttatcaca aggagaccat tgccccggat ttatccgatg aagaacttgc ggatctcttt
     1321 tacagccacc tggagagtga aaattatccc ggcgcgctaa ctgtcaccca acataccatt
     1381 aatacttcgc cgcctttcct tcggttaatg ggcacgccta cgagcatccc gaatgatacc
     1441 tggttacagg ggcattcgtt tctctcaacc gtagcaaaat atagtaatga tcttcctcag
     1501 gctgaaatga tagccagaga gcttgcttat aacgcaccag gaaatcaggg actgcgcatt
     1561 gattacgcga gtgtgttaca agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa
     1621 aaagcagaag tgatcgagcc acgtaatatt aatctggagg ttgaacaagc atggacagca
     1681 ttaacgttac aagaatggca gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa
     1741 ccgcaagatc ccggcgttgt acgtttaaaa cgtgcggttg atgtacataa tcttgtagag
     1801 cttcgtatag ctggttcaac aggaattgat gccgaaggcc cggatagtgg taaacatgat
     1861 gtcgacttaa ccaccatcgt ttattcacca ccgctgaagg ataactggcg cggttttgct
     1921 ggattcggtt atgccgatgg acaatttagc gaaggaaaag ggattgttcg cgactggctg
     1981 gcgggtgttg agtggcggtc acgtaatatc tggctcgagg cggagtacgc tgaacgcgtt
     2041 ttcaatcatg agcataaacc cggcgcgcgc ctgtctggct ggtatgattt taatgataac
     2101 tggcgtattg gttcgcaact ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa
     2161 aatggtgtta caggcaacag tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt
     2221 aagtacggtg tctcctgggc tttcactgat ttttccgaca gtaaccagcg tcaggaagtc
     2281 tcacttgagg gtcaggaacg catctggtct tcaccatatt tgattgtcga tttcctaccc
     2341 aatctgtatt acgaacaaaa tacagaacac gacactccat actacaaccc tataaaaacg
     2401 ttcgatattg ttccggcatt tgaggcaagt catttgttat ggcgaagcta tgaaaatagc
     2461 tgggagcaaa tcttcagcgc aggtgttggt gcctcctggc aaaaacatta tggcacggat
     2521 gtcgtcaccc aactcggcta cgggcaacgt attagctgga atgacgtgat tgatgctggc
     2581 gcaacgctac gctgggaaaa acgaccttat gacggtgaca gagaacacaa cttatacgtt
     2641 gaattcgata tgacattcag attttaagga taaatatgtt acgtaatgga aataaatata
     2701 tcctgatgct ggtgagtata attatgctca ctgcgtgcat tagccagtca agaacatcat
     2761 ttataccgcc acaggatcgc gaatctttac tcgccgagca accgtggccg cataatggtt
     2821 ttgtggcgat ttcatggcat aacgttgaag acgaagctgc cgaccagcgt tttatgtcag
     2881 tgcggacatc agcactgcgt gaacaatttg cctggctgcg cgagaacggt tatcaaccgg
     2941 tcagtattgc tcaaattcgt gaagcacatc gaggaggaaa accgctaccg gaaaaagcag
     3001 tagtgctgac ttttgatgac ggctaccaga gtttttatac ccgcgtcttc ccaattcttc
     3061 aggccttcca gtggcctgct gtatgggccc ccgtcggcag ttgggtcgat acgccagcgg
     3121 atgaacaagt aaaatttggc gatgagatgg tcagtcgaga atattttgcc acgtggcaac
     3181 aagtgcgaga agttgcgcgt tcccggctcg ttgaggtcgc ttctcataca tggaattctc
     3241 actacggtat tcaggctaat gccaccggca gcttattgcc tgtatatgta aatcgtgcat
     3301 attttactga ccacgcacgg tatgaaaccg cagcagaata ccgggaaaga attcgtctgg
     3361 atgctgtaaa aatgacggaa tacctgcgta caaaggctga ggtaaatcca cacgtttttg
     3421 tttggcctta tggcgaagcg aatggcatag caatcgcgga attaaaaaaa ctcggttatg
     3481 acatgttctt cacccttgaa tcaggtttgg caaatgcgtc gcaattagat tccattccgc
     3541 gagtattaat cgccaataat ccctcattaa aagagtttgc ccagcaaatt attaccgtac
     3601 aggaaaaacc actacaacgg gtaatgcata tcgatcttga ttacatttat gacgaaaacc
     3661 gccagcaaat ggatcgcaat attgatgtgc taattcagag ggtaaaagat atgcaaatat
     3721 ctaccgtgta tttacaagca tttgctgatc ccgatggtga tgggctggtt aaagaggtct
     3781 ggtttccaaa tcgtttgcta ccaatgaaag cagatatttt tagtcgggtt gcctggcaat
     3841 tgcgtacccg ctcaggtgta aacatctatg cgtggatgcc ggtattaagc tgggatttag
     3901 atcccacatt aacgcgagta aaatacttac caactgggga gaaaaaagca caaattcatc
     3961 ctgaacaata ccgccgtctc tcccctttcg atgacagggt cagagcacaa gttggcatgt
     4021 tatatgatga tcttgccgga catgctgctt ttgatggcat attgttccac gatgatgctt
     4081 tgctttcaga ttatgaagat gccagtgcag cggctatcgc ggcttatcag caagctggct
     4141 ttagcgggag tctgagcgaa attcgacaaa acccggagca attcaaacag tggacccgct
     4201 ttaaaagtcg tgcgttaact gacttcactt tagaacttag tgcgcgcgta aaagccattc
     4261 gtggtccaca tgttaaaact gcacgaaata tttttgcact tccggtaata caacctgaaa
     4321 gtgaagcttg gtttgcacag aattatgctg atttcctaaa aagctatgac tggaccgcta
     4381 ttatggctat gccttatatg gaaggcgtca cagaaaaatc ggctgatcaa tggttaacac
     4441 aattgaccaa tcagatgaaa agcatcccgc aggctaaaga caaatcaatt ttagaattac
     4501 aggcacaaaa ctggcagaaa aatggtcagc atcaggctat ttcttcacaa caactcgctc
     4561 actggatgag cctattacaa ctgaatggag tgaaaaatta tggttattat cccgacaatt
     4621 ttctgcataa ccaacctgaa atagacgtta ttcgtcctga gttttcaaca gcctggtatc
     4681 cgaaaaatga ttaatcgcat cgtatcgttt tttatattat gtctggtgtt atgcatacct
     4741 ctatgcgtag cgtactttca ctctggtgaa ctgatgatga ggttcgtttt cttctggccg
     4801 ttttttatgt ccattatgtg gattattggc ggcgtctatt tctgggtcta tcgtgaacgc
     4861 cactggccgt ggggagaaaa cgcaccagcc ccccagttga aagataatcc atctatctcc
     4921 attatcattc cctgttttaa tgaggagaaa aacgttgagg aaaccataca tgccgcttta
     4981 gcacagcgtt atgagaacat tgaagttatt gccgtaaatg atggttcaac agacaaaacc
     5041 cgtgccatcc tggatcgcat ggctgcacaa attccccgtc tgcgggtcat tcatctggcg
     5101 caaaaccagg ggaaagccat tgcgcttaaa accggagctg ccgcggcgaa aagtgaatat
     5161 ctggtgtgca ttgatggcga cgcgttatta gaccgcgatg cagcggcata tattgtggaa
     5221 ccgatgttgt acaacccacg tgtgggtgcc gtaaccggta atcctcgtat tcgcacacgt
     5281 tctaccctag tgggtaaaat tcaggttggc gagtattcct caattattgg tttgatcaag
     5341 cggacccagc gtatctatgg aaatgtattt accgtttccg gtgttattgc cgcatttcgt
     5401 cgcagcgccc tggcagaagt gggttactgg agtgacgata tgatcaccga agatattgat
     5461 attagctgga agctgcagtt gaatcagtgg acgatttttt acgagccacg ggcgctgtgc
     5521 tggatattaa tgcctgaaac gttaaaaggg ctgtggaaac agcgcctgcg ctgggctcag
     5581 ggcggtgcag aagtattcct caaaaatatg acaaggttgt ggcgaaaaga aaactttcga
     5641 atgtggccac tgttttttga atactgcctg acgacaatat gggcctttac ctgcctggtc
     5701 ggtttcatta tttacgcagt ccaacttgca ggtgtaccgt taaatattga attgacacat
     5761 atcgcagcga cacatactgc cggaatatta ttgtgcacgt tatgtttact gcaatttatt
     5821 gtcagtctgg taattgagaa tcgctatgag cacaatctgg cttcatcgct tttctggatt
     5881 atttggtttc cggttatttt ctggatgcta agcctggcaa cgacattggt atcatttaca
     5941 cgagtcatgt tgatgcctaa aaaacaacgc gctcgttggg taagtcccga tcgtgggatt
     6001 ctgagaggtt aatatgaaca atctaattat tacgacccga caatcgccag tacgtttact
     6061 ggttgattat gttgccacaa ccatcttgtg gacattattt gctttgttca tattcttatt
     6121 cgccattgat ctgctgacgg gttattactg gcaaagcgag gccagaagcc gacttcagtt
     6181 ctattttttg ctggcagtgg cgaatgccgt cgtgttaatc gtctgggcat tgtacaataa
     6241 gctgcgtttt aaaaaacagc agcatcatgc agcataccaa tatacgccgc aagaatatgc
     6301 agagagctta gcaatacctg atgagcttta tcagcaacta caaaaaagcc acaggatgag
     6361 cgtacacttc accagtcagg gacaaataaa aatggtggtt tcagaaaaaa cgctagtccg
     6421 ggtataaaca cccaaaacaa agcccggttc gcccgggctc tgcaccgata atacacttaa
     6481 ctgtatgcat gtagcgtacg ttggcaaagt gccgaacgta cgcaatcctc tttaccgaat
     6541 cggatgatcc cgaccatttc atcttcttcg aaacgttcca gcgcgtcact taatccggag
     6601 cgcacaccgc gaggtaaatc gcattgcgtg atatcaccgt tgacgataac cgtcacgttc
     6661 tccccgaggc gggttaaaaa cattttcatt tgcgcggcag tcacattctg cgcctcgtca
     6721 agaatgacga ctgcattttc aaaggtacgt ccacgcatat aggcgaacgg cgcgatttcg
     6781 accttcccta tttctggtcg caggcagtac tgcataaagg aagcccctaa gcgccggacc
     6841 agcacgtcgt aaaccgggcg aaaataggga gcaaactttt ctgcgatatc tccaggtaaa
     6901 aagccaagat cttcatcggc ttgcagaact ggacgggtga cgataatcct gtcgacatcc
     6961 ttatgtatca gggcctctgc cgcttttgct gcgctgatcc aggtttttcc gcacccggct
     7021 tcgcccgtgg cgaatatcag ctgcttactc tcaatagcct tcagatagtg caattgcgct
     7081 tcatttcgcg cgaggatggg cgaggtatcg cgactgtcgc gggccatacc aatggcttct
     7141 acgccgctca tttgcacaag cgaggtgacc gattcttctt cacgctgctt atggctgcgc
     7201 gaatcccgtc tcagcacacg ttttgcctcg cgacgagctt tgatcactgc tttttgtctt
     7261 cccatggaga gcaccttgag ttgtttgtat tcatcacacg cgccgttggc agcgcgatta
     7321 tgcgcacgaa catcagaggg ttggcttcct tgtaagccat agtttgcttt tggataaaat
     7381 gccgaaaaac ggctacgcac accgtttgcg gcgtcggtaa cacgagtaaa gagaggaagt
     7441 gagaaaaata cagagtgacg aagttgctac cggaggagaa atctccgcga gtggtggagc
     7501 cttgcttaac aaaacattgt ccagaacagg acgttaccat ccgcgatctc catagtgact
     7561 gactatcact gccgggaact tccgctgcta cttaataagt acaacagatc tcgcattcat
     7621 tgcaacaata tattcactta tatttaacca taaaacacca tttcagtgac attagtttct
     7681 actggaaaga tgacaaagtg atgacagtga taaaaaagct gtgtgctttc agcaggattc
     7741 gcagtactgg taaaagcttg ccggatgcgg cgtgaacgcc ttatccggcc taccgaaccc
     7801 gcaccgattg taggcatgat aagacgcgca agcgtcgcat cagcgcacga acgcctggtg
     7861 cggcgtaaac gccttatccg cctaaaaaca ttaaacccgc aataatgcgc ttccgagata
     7921 atcgttcgcg tccttcacac ccggcagcgc aaagaaatag ccgccaccga taggtttgac
     7981 atattcctcc agcgcctctc cattcaacct tttttgtact gtcaggaagc ccttttccag
     8041 atcgtgttgg tagcagacaa acagtagccc catatcgagt tgcccggaat tggtgacgcc
     8101 caacgaataa ctgtagccac gacgcagcat cagacttgac tcactctctg gcgtgcgggg
     8161 attcgccagc cggatatggc tgtccagcgc gatcaccttc ccctccggat cgctggcgta
     8221 atcaggcaca tcatgctcat gctgcattcc cagcggcgcg ccggtttgct tatcacgccc
     8281 aaatatcgtc tgctgttctt tcagcggcgt tctgtcccaa aattccaccc gaaactgaat
     8341 caaccgcacc gcctggtagc taccaccgat tgtccacgca ggctcctgct gatctgccgt
     8401 cacccacacc actttttgca tcaacttcgc attctggcta tcgggattgg cagtgccgtc
     8461 tttgaaaccc agcaaattaa tcggtgtctc tttaccttta ctacgcgccg cgtggtcgga
     8521 aataacccct tcccgcttcc agcgcacgct gagcaaatcc ggcgtgtgtt tgatgatatc
     8581 gcgcagcgca tggatcaccg tgtcctgggt gttggcgcaa atttgtagca acacatcacc
     8641 atgacataac gccgcatcca gcgagtcgtt ggggaaacgc gtcatcttct gcaacttttt
     8701 cggcatctgt ggcgcaaggc caaagcgctc atcaaacaat gagtggccca ccgataacgt
     8761 tatggtgaga ttatcgggcg caatgtagcc gccaagaatg ccggaatcga gtggtggcag
     8821 gcgcggattt ggcgtttctg gtgccgctcc gccctgagtc agaaaagcaa aacgctgagt
     8881 caacaagcga aacaaccgtt caagatcggc tttatcgctg gcaagcacat caaacgccac
     8941 cagcatcatt gcagcctgtt gtggcgtcag gatccctgcc tgatgctcac cataaaacgg
     9001 ctgtttctca ttgcgtgcat ccggtgaaag cgtacccggc gcactttgcg ttttttgtgc
     9061 atgagcgacc ggacaacttc ccgccagcgc cagcgcacca atccctttca gtaaacgtcg
     9121 gcgtgacggt tcattcacgc cgttttcatc ttcatactgc ataacgctta gtccagcccc
     9181 agcacaccgc gaagttgcgc cagatcttcc gccagcgcag taatcggtcc tttcagtgca
     9241 ttccggtcag catcggtcag tttgtcgtaa ttttcaaaac cgtctttagt acggtatttc
     9301 gccagaatgg tatcgacctt tatgaagttg gcatcgactt ttgccaacag ttccggattc
     9361 actttttgta gttgtggacg cagcagatcg acaattttct gcgagccttc aacgttagcc
     9421 tggaaatccc acagatcggt gtggctgtag cgatcttctt caccgctgat tttgctggcc
     9481 gccacttcct caatcagtcc ggctgcaccg ccgaccactt ttgaaggagg gaaagccagt
     9541 tcactgatgc gtttttgcaa atcgaccaca tcggtataaa gctggtcggc atacttatcc
     9601 atccctttgg tggtgttatc gccaaacaat gctttttcca gacggtggaa accggtgaat
     9661 ttcggatcgg cggctttttg ctcgtaatca tcttcacggg cgtcaatgct gccatccaga
     9721 tcggagaaca gttcagcaat cggttcaatg cgctcatagt gctggcgcgt cggcgcatac
     9781 agtgctttcg ctttttcgat atcgcctgct ttaatagcat cggtaaaggc tttggtgtcg
     9841 gtcaccagct gcgtggtttc cgccatgaca tacgctttat atgcagtaat tgcaccacca
     9901 agacttaaca gcgcatcact ttgcgccgca tccgccgttg cctcaccttt gacgatcaac
     9961 ttccctttcg ggtttgtcag cagaccgcag gtcatatcgt attcaccagg ctgtaaattt
    10021 gccgtcattt tctggctaaa accaggggcg atattttccc gctcttccac caccatcacg
    10081 cctttgagga tctcccactc cagtgctttc tggctgtggt tctgaataat gaactgtgtt
    10141 ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct tatccgtcac ggtcactttg
    10201 acctgcggca catcagcggc gttagccata aaagcagaag aaaacagcgc agccacgctc
    10261 aactgcaatg cgttacggcg gaagttaatg gtcatgacga gactatccct ttaaagtatg
    10321 ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg agcgagacgc tgtcgcccct
    10381 gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga gataaataaa ccagacggcg
    10441 acttcgctga cgctcggcgc ttcctgatag ccaaaaatcc cttccatcag cgtgccaaac
    10501 accgagtgag ttgagagcac cgcactcata tcgaaggcga tttcctgaaa gtggttccac
    10561 aatccggctt catgaaatgc gcgaatggca ccagccgcca gccctgcggc gacgaagaga
    10621 ataaacaggc tggtccattt aaaaaatgca ccaagattga ggcgaatacc gccccagtag
    10681 agcaggaagc cgagcaccac ggctgtagca agaccgagca ttgcacccag cggcggccag
    10741 atcccgacat cttgttgaaa cgccgccagc aggaaaaaga ccgactccag cccttccctt
    10801 gcaacggcaa aaaagaccat catcaccagc gcccagccat gatgatttcc acgctgcaat
    10861 gcgctatcga ctgcctgctc cagttgtact ttgacgttgc gcgacacttt gcgcatccag
    10921 aaaaccatcc aggtaaggat caccacggcg atcaccgcca cgataccttc aaacagttcc
    10981 tgttcttttt gcggaaattc gccggtggtt tcgttaatga agatacccaa gcccaggcac
    11041 aacgcagcgg caagcaacac gccaatccac atcacaccaa tccatcggcc tcgctgggta
    11101 cgcttaagat agctggcaat caaactgaca atcagcgcgg cttcaagtcc ttcgcgcaac
    11161 ataatgagaa acggaacaaa catgccaccc acccttaaac gtttttctga gtcaatctat
    11221 gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac ggtaaagaaa aatacaagca
    11281 gaatgtttat aaaatgatgt tggaatgtaa acaaagaaag gtaatgccag gggatataaa
    11341 cgattattcc aggtacaaag atgggattgt ttacaacgag ccatgtgttc ttgccagatg
    11401 ccgcgtaaat gccttatcca gccgacaaaa ctgtccagac tcaatgcatc agagcttcca
    11461 tgtaaaactg ataagcgggt gtatcaagca atttcgtcca tccgcgatta tcaaatctaa
    11521 ctttttgccc ggaacataat aacagctcgg gtgttggtgg cgtcaaaatc gctgaagcgt
    11581 tccccgaagg ccggggcagc ccacatggat gtgggctgag ggcgcgtctt acaggacgtt
    11641 acctcgcgcc cgacccggta gccgaagggg ataagctgaa ggtaccgcgc agcggcgatt
    11701 ttgttcgcca gagcccgggg gtgcaggggg cggcggcgat tggccgcccc ctgcgcgctc
    11761 cctgcaccat tcaatttata tggcgaggca cattaaggga gcgcaacccg ctgctgaagc
    11821 caaacaaaac cgccgcaggc taagtccctt acccttcctg caaccgtgac ggcggagccg
    11881 aatgatagtg cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac ggcgctttac
    11941 ccagcaaact aaacactaca atcccgatac tgccgaagat aaagcccgga ataatttcgt
    12001 acagacccag ccagccgaac tgtttccaga cgataaccgt cagcgcaccg atgatcatcc
    12061 ccgccagcgc accgttgcgc gtcatgcgtg accacatcac cgagaacagc accaccggac
    12121 caaacgctgc gccaaagcct gcccacgcgt agctcactaa gcccagcacg cggttttccg
    12181 ggtttgccgc cagcgcaatc gccaccagcg ccaccaccag caccatcact cgccctaccc
    12241 acaccagctc tttctggctg gcctgtttac gcagaaacgc tttgtataaa tcttcggtaa
    12301 tcgcactgga acataccagc agctggcaac ttaacgttga cattaccgcc gccagaatcg
    12361 ccgacagcaa aatcccggca atccacgggt taaacagaat ttgcgccagt tcaataaaca
    12421 cgcgttcagc gttctggttt accgcacctg ctaccgccgg gtgctcgtta aagtaagcga
    12481 taccaaagaa gccgacagcc accgcccctg ccaggcagag gatcatccag gtcatactga
    12541 tacgacgcgc atggacaatg ctgtggtgag aatctgccgc cataaaacgc gccaggatgt
    12601 gcggctgccc gaagtaaccc agcccccacc ccatcagcga gataatggcg acaaaattca
    12661 accctttgag catatccacg ttttcgatgc tcttttgttt gattacttcc agcgagtcgc
    12721 caaacccacc gacgctaatg atcacgataa ccggcgtcag gatcagggca aaaatcatca
    12781 ggctggcctg tacagtgtca gtccagctca ccgcgaggaa accgccaata aaggtgtaaa
    12841 ggatcgtcgc cgcagccccc gcccacagag ccgtttcgta gctcatgcca aaggtacttt
    12901 caaacagacg cgcgcctgcc acaatgcccg aagcgcaata aatggtgaag aacagcaaaa
    12961 taaccagcgc ggagataatg cgcaaaatgc ggcttttatc ttcaaagcgc ccggtgaaat
    13021 aatccggcaa tgttaaggcg ttattgttgt attcggtatg cacacgcaac cgcccggcca
    13081 ccagcttcca gtttatccac gcgcctaatg tcaggccaat ggcgatccag ctttcggaaa
    13141 tcccggaaag aaaaaccgcg cccggcaacc ccattaacag ccagccgctc atatccgacg
    13201 ctcccgccga taatgccgtc acgaatggcc caagactacg accgcccaga atatagtcgt
    13261 cgaagttttt cgttgatcgc caggcgataa acccaatcaa tatcatgcca aagatataga
    13321 cacaaaatgt caccaacatc ggtgtgctaa tagccatcta aagtctccaa aaaattatta
    13381 tcggcaatgt cgaaacttgc cgttatatct gccaccggaa cggggtaaca gagtttatgt
    13441 tttaccaggg cgaccgtatc ctgccggaag cgctggttat tcacaatcga tttaacacac
    13501 catttacatt aaattttagt gctcagcgac actatttttc atcaggttgc actctctcac
    13561 attttttgcg gttgcacctt tcaaaaatgt taactgccgc agagaaaaag tctgagttat
    13621 ttttttaatc cctgtcatat cgatttcgtt tattaacatt tcattcattt ttaagcttgc
    13681 tacgcaggtc acatttaaca cagttgcaca aagttgcaac atcatggata tttcacgata
    13741 acgttaagtt gcacctttca gaacaacagg agtaatggca tgggaaccac caccatgggg
    13801 gttaagctgg acgacgcgac gcgtgagcgt attaagtctg ccgcgacacg tatcgatcgc
    13861 acgccacact ggttaattaa acaggcgatt ttttcttatc ttgaacaact ggaaaacagc
    13921 gatactctgc cggagctacc tgcgctgctt tctggcgctg ccaatgagag cgatgaagca
    13981 ccgactccgg cagaggaacc acaccagcct ttcctcgact ttgccgagca aattttgccc
    14041 cagtcggttt cccgcgccgc gatcaccgct gcctatcgcc gtccggaaac cgaagcggtt
    14101 tcgatgctgc tggaacaagc tcgcctgccg cagccagttg ctgaacaggc gcacaaactg
    14161 gcgtatcaac tggccgataa actgcgtaat caaaaaaacg ccagtggtcg cgcaggtatg
    14221 gttcaggggt tattgcagga gttttcgttg tcatcgcagg aaggcgtggc gctgatgtgt
    14281 ctggcagaag cgttgttgcg tattcccgac aaagccaccc gtgatgcgtt aattcgcgac
    14341 aaaatcagca acggtaactg gcagtcacac attggtcgta gcccgtcgtt gtttgttaat
    14401 gccgccacct gggggctgct gtttaccggc aaactggttt ccacccataa cgaagccagc
    14461 ctctcccgct cgctgaaccg cattatcggt aagagcggtg aaccgctgat ccgcaaaggt
    14521 gtggatatgg cgatgcgcct gatgggcgag cagttcgtca cgggtgaaac cattgcagaa
    14581 gcgttagcca atgcacgcaa gctggaagag aaaggtttcc gttactccta cgatatgctg
    14641 ggcgaagccg cgctgaccgc cgcagatgca caggcgtata tggtttccta tcagcaggcg
    14701 attcacgcca ttggtaaagc gtccaatggt cgtggcatct atgaaggtcc gggcatttca
    14761 atcaagctgt cggcgctgca tccgcgttac agccgcgccc agtatgaccg ggtaatggaa
    14821 gagctttacc cacgtctgaa atcactcacc ctgctggcgc gtcagtacga tattggtatt
    14881 aacatcgatg ccgaagaggc cgatcgtctg gagatctccc tcgatctgct ggaaaaactc
    14941 tgtttcgagc cggaactggc aggctggaac ggcatcggtt ttgttattca ggcttatcaa
    15001 aaacgctgcc caccggtgat tgattacctg attgatctcg ccacccgcag ccgtcgccgt
    15061 ctgatgattc gcctggtgaa aggcgcgtac tgggatagtg aaatcaagcg cgcgcagatg
    15121 gacggccttg aaggttatcc ggtttatacc cgcaaggtgt ataccgacgt ttcatatctc
    15181 gcctgtgcga aaaagctgct ggcggtgccg aatctaatct acccgcagtt cgcaacgcat
    15241 aacgcccata cgctggcggc aatttatcaa ctggcgggtc agaactacta cccgggtcag
    15301 tacgagttcc agtgcctgca cggtatgggc gagccactgt atgagcaggt caccgggaaa
    15361 gttgccgacg gcaaacttaa ccgtccgtgt cgtatttatg ctcctgtcgg tacccatgaa
    15421 acgctgctgg cgtatctggt gcgtcgcctg ctggaaaacg gtgctaacac ctcgtttgtt
    15481 aaccgtattg ctgacacctc tttgccactg gatgaactgg ttgccgatcc ggtcactgct
    15541 gtagaaaaac tggcgcaaca ggaagggcaa actggattac cgcatccgaa aattcccctg
    15601 ccgcgcgatc tttacggtga cgggcgcgac aactcggcag ggctggatct cgccaatgaa
    15661 caccggctgg cctcgctctc ttctgccctg ctcaatagtg cactgcaaaa atggcaggcc
    15721 ttgccaatgc tggaacaatc ggtcgcggct ggtgagatgt cgcccgttat taaccctgcg
    15781 gaaccgaaag atattgtggg ctttgtgcgt gaagccacgc cgcgtgaagt agaacaggcg
    15841 ctggaaagtg cggttaataa cgcgccaatc tggtttgcca ctcctccggc tgaacgcgcg
    15901 gcgattttgc atcgcgctgc cgtgctaatg gaaagccaga tgcagcaact gattggtatt
    15961 ctggtgcgtg aagccggaaa aaccttcagt aacgccattg ccgaagtgcg cgaagcggtc
    16021 gattttctcc actactatgc cggacaggtg cgggatgatt tcgctaacga aacccaccgt
    16081 ccattagggc ctgtggtatg tatcagtccg tggaacttcc cgctggctat tttcaccggg
    16141 cagatcgctg ccgcactggc ggcaggtaac agcgtgctgg caaaaccggc agaacaaacg
    16201 ccgctgattg ccgcgcaagg gatcgccatt ttgctggaag cgggtgtacc gccaggcgtg
    16261 gtgcaattgc tgccaggtcg gggtgaaacc gtgggcgcgc aactgacggg tgatgatcgc
    16321 gtgcgcgggg tgatgtttac cggttcaacc gaagtcgcta cgttactgca gcgcaatatc
    16381 gccagccgcc tggacgctca gggtcgccct attccgctca tcgctgaaac cggcggcatg
    16441 aacgcgatga ttgtcgattc ttcagcactg accgaacaag tcgtaataga tgtgctggcc
    16501 tcggcgttcg acagtgcagg tcagcgttgt tcggcactgc gcgtgctgtg cctgcaagat
    16561 gagattgccg accacacgct gaaaatgctg cgcggcgcaa tggccgaatg ccggatgggt
    16621 aatccgggtc gcctgaccac cgatatcggt ccggtgattg atagcgaagc gaaagccaat
    16681 attgagcgtc atattcagac catgcgtagt aaaggccgtc aggtgttcca ggcggtgcgg
    16741 gaaaacagcg aagatgcccg tgaatggcaa agcggcacct ttgtcgctcc gacgctgatc
    16801 gaactggatg actttgccga attacaaaaa gaggtctttg gtccggtgtt gcatgtggtg
    16861 cgttacaacc gtaaccagct gccggcgctg atcgagcaga ttaacgcttc cggttatggt
    16921 ctgacgcttg gcgtccatac gcgcattgat gaaaccatcg cccaggtcac tggctcggcg
    16981 catgtcggta acctgtacgt taaccgtaat atggtgggcg cagtggttgg ggtgcaaccg
    17041 ttcggcggcg aagggttgtc cggtaccggg ccgaaagcag gcggtccgct ctatctctac
    17101 cgtctgctgg cgaatcgccc ggaaagtgcg ctggcagtga cgctcgcgcg tcaggatgcg
    17161 gagtatccgg tcgatgcgca gttgaaagcc gcattgactc agccgctaaa tgcactgcgg
    17221 gaatgggcgg caaaccgtcc agaattgcag gcgttatgta cgcaatatgg cgagttggcg
    17281 caggcaggaa cacaacgatt gttgccaggg ccgacgggtg aacgcaacac ctggacgctg
    17341 ctgccgcgtg agcgcgtgtt gtgtattgcg gatgatgaac aggatgcgct gactcagctc
    17401 gccgccgtgc tggcggtggg tagccaggtg ctgtggccgg atgatgcgct gcatcgtcag
    17461 ttagtgaagg cattgccatc ggcagtcagc gaacgtattc aactggcgaa agcggaaaat
    17521 ataaccgctc aaccatttga tgcggtgatc ttccacggtg attcggatca gcttcgcgca
    17581 ttgtgtgaag ccgttgccgc gcgggatggt gcaattgttt cggtgcaggg ttttgcccgt
    17641 ggcgaaagca atatccttct ggaacggctg tatatcgagc gttcgctgag tgtgaatacc
    17701 gctgctgctg gcggtaacgc cagtttaatg acgatcggtt aaacaggtag ccggaggatg
    17761 ttacaacctc ctccggcatc tttaacgtgg tcgaatcccc tcaataataa tccgctgcac
    17821 gttttcaacc gtttgattga aaaacacctc atcgcgcaac gtcgcgcctg tcaccgcctc
    17881 cacctgaggg gcaaaatcgg cgtaatgttg agtggaagcc caaatcataa aaatcaaatg
    17941 ctgcgggtca atcggcgcga gtttgccgct tttaacccaa ccggcaatga gcgccgattt
    18001 ctcatcaatt aatgccttca aatcgcccgt cagttcatcc attaacagcg gtgcacctgc
    18061 cagcatctcc atacagaaca ggcgcgaagc ctgcggataa tcgcgggaaa cttccagctt
    18121 cagacggatg tattctttga tcgccgccag cggggcgaaa tcttctcgaa acgcttttaa
    18181 cggtgccagc cagatatcga gaatctgccg cagtacggct atatacagcg cctcttttga
    18241 cggaaagtaa tacagcagat tggtttttga aacacccgcc aactccgcga tctgctccag
    18301 ccttgtgccg tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa gaatcgcttt
    18361 tttcttcgcg cttactgcgc gcgaacgctt acccgttgtt ttcactgcgc cttgcgtcat
    18421 gcgctctccc ctcttgttga ttggcaacag gatagcaaaa cccccgcccg gcctcgacct
    18481 tctgcactct catcgcgcac gaatgcatgt tttatgtgca actgttttga ccgtttagtc
    18541 cacttttttt caggcatttt agacaactta acattaacac attaataaca ttcacttttt
    18601 aaaaactggc atccgctttg caaacaagcc attacgcggc tgaaaaggat atgcaggatg
    18661 cagcgccccg tctgactttc actttacgag acgaagagag gttgatgatg aaaattggcg
    18721 tattcgtacc tattggcaac aacggctggc tcatttcgac ccacgcgccg cagtacatgc
    18781 cgacatttga actgaataaa gccattgtgc aaaaagcgga gcactaccat ttcgatttcg
    18841 ccctgtcgat gatcaaactg cgtggctttg gcggcaaaac tgagttctgg gatcacaacc
    18901 ttgagtcgtt caccttgatg gcggggctgg cagccgtcac ctcacgcatt cagatatacg
    18961 cgacagctgc caccttaacg ttacctccgg caatcgtcgc ccgtatggcc gcaaccatcg
    19021 actccatctc tggcgggcgt tttggcgtca acctcgtgac tggctggcag aagcccgagt
    19081 atgagcagat ggggatctgg cctggcgatg actatttctc ccgtcgttac gactctctca
    19141 ccgaatatgt tcaggtgctg cgcgacctgt ggggctcggg gaaaagcgat tttaaaggcg
    19201 attttttcac tatggatgat tgtcgcgtca gtccgcaacc gagtgtcccc atgaaagtga
    19261 tctgcgccgg gcaaagcgac gctggcatgg cgttctccgc ccggtatgcc gattttaact
    19321 tctgtttcgg caaaggcgta aatacaccca cggctttcgc cccgaccgct gcgcggatga
    19381 aacaggccgc agagcaaacc ggacgcgacg ttggctctta tgtgttgttt atggtgattg
    19441 ccgacgaaac cgacgatgcc gctcatgcaa aatgggaaca ctacaaagcg ggcgcggatg
    19501 aagaggcgtt aagctggcta accgaacaaa gtcagaaaga tacccgctcc gatactgaca
    19561 ccaacgtccg tcagatggcc gatcccactt cggcggtaaa catcaatatg gggacgttag
    19621 tcggttctta cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgccg
    19681 aaggcgtgct gttgaccttc gatgattttc tgtcgggaat cgaaaacttc ggcgagcgca
    19741 ttcaaccact gatgcagtgc cgcgcccatc tccctgcgct gactcaggag gtggcatgat
    19801 gacgacctta accgcacgac cggaagccat taccttcgat ccgcagcaaa ctgcgctgat
    19861 cgtggtggat atgcaaaacg cttatgccac gccaggcggc tacttagatc tcgccgggtt
    19921 tgatgtctca accactcgcc cggtcattgc caacattcaa accgccgtga ccgcagcgcg
    19981 agcggcaggg atgttgatca tctggtttca aaatggctgg gatgaacagt atgtcgaggc
    20041 tggcggcccc ggctcaccga attttcataa atcgaacgcc ctgaaaacca tgcgtaagca
    20101 gccgcagctg caggggaaat tgctggcgaa aggctcctgg gattatcaac tggtggatga
    20161 actgatgccg cagcctggcg atattgtgct gccgaagccg cgctacagcg gtttcttcaa
    20221 tacgccgctg gacagcattt tgcgcagccg tggaatacgc catctggttt tcaccggtat
    20281 cgctaccaac gtctgcgtcg aatcgacgct acgcgacggc ttttttctgg agtatttcgg
    20341 cgtggtgctg gaagacgcaa cacaccaggc gggaccggaa tttgcacaga aagccgcgtt
    20401 gttcaatatc gaaacctttt ttggctgggt cagcgacgtc gaaacgttct gcgacgcgct
    20461 ttcttccacg tcctttgctc gtatcgctta aggagtttaa cgatgccaaa atccgtaatt
    20521 attcctgctg gcagcagcgc accgctggcc cccttcgttc ccggcacgct ggctgatggc
    20581 gtggtgtatg tctccggtac gctggctttt gatcaacata acaacgtgct gtttgccgat
    20641 gacccaaagg cgcaaacccg ccacgttctg gaaaccatcc gcaaggtcat cgagacggcg
    20701 ggtggcacaa tggcggatgt gaccttcaac agcatcttta ttaccgactg gaaaaattac
    20761 gccgcgatta acgaaatcta cgccgaattt ttcccgggtg acaaaccggc gcgattctgc
    20821 attcagtgcg gactggtaaa acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc
    20881 gccaagtgag gccgcgatga aactttcact ctcacctccc ccttatgctg atgcgcccgt
    20941 agtggtgttg atttcgggtc ttggcggtag cggcagttac tggttaccgc aactggcggt
    21001 gttggttcag gagtatcagg tagtctgtta cgaccagcgc ggcaccggca ataatcccga
    21061 cacgctggca gaagattaca gtatcgccca gatggcagcg gaactgcatc aggcgctggt
    21121 agccgcaggg attgagcgtt acgcggtggt cggccatgcg ctcggtgcgc tggtgggaat
    21181 gcagctggcg ctggattatc ccgcgtcggt aactgtgctg gtcagcgtta acggctggct
    21241 acgaataaac gcccatacgc gccgctgttt tcaggttcgc gaacagttac tacatagcgg
    21301 cggcgcgcag gcatgggtgg aagcgcagcc gttgttcctc tatcccgccg actggatggc
    21361 ggcccgcgca cctcgccttg aggcagaaga cgcgctggca ctggcgcatt ttcaaggcaa
    21421 aaataattta ctgcgtcgac ttaacgccct caaacgcgct gactttagtc accatgcgga
    21481 tcgtatccgc tgcccggtgc aaatcatctg cgccagtgat gatctgctgg tgccaacagc
    21541 atgttccagt gaacttcatg ccgccctgcc cgatagccag aaaatggtga tgcgctatgg
    21601 cggacacgcc tgcaacgtga ccgatcccga aacgtttaat gctctgttac tcaacgggct
    21661 tgccagcctg ttacatcacc gtgaagccgc tctgtaagga attgctatga acgaagccgt
    21721 tagcccaggt gcgcttagca ccctgttcac cgatgcccgc actcacaacg gctggcggga
    21781 gacacccgtc agcgatgaga cgttacggga gatttatgcc ctgatgaaat gggggccgac
    21841 atcagctaac tgttctccgg cacggatcgt gtttatccgc actgcagaag gaaaagaacg
    21901 tctgcgcccg gcgctttcca gcggcaatct gcaaaaaacc ctgaccgcgc ccgtcaccgc
    21961 tatcgtcgcc tgggacagtg aattttatga acggttacca cagctgtttc ctcacggcga
    22021 tgcccgcagt tggtttacct ccagtccaca acttgccgaa gaaacagcgt ttcgcaacag
    22081 ttccatgcag gcggcctatc tgatcgtcgc ctgccgtgcg ctggggctgg ataccggccc
    22141 gatgtcgggc tttgaccgtc aatacgttga cgacgccttt tttgcgggca gcacgctgaa
    22201 gagcaatctg ctgattaata tcggctatgg cgattccagc aaactttttg cgcgcctgcc
    22261 acgtctgtcc tttgaagaag cctgcgggct gttgtaagga gcaaacaatg aacattgtcg
    22321 atcaacaaac ctttcgcgat gcgatgtcct gtatgggcgc ggcggtcaat atcatcacta
    22381 cggacggtcc agccgggcgc gccgggttca ccgccagcgc cgtctgcagc gtgactgaca
    22441 cgccgcccac attactggtg tgcctgaatc gtggggcgtc cgtctggccg gtattcaatg
    22501 aaaatcgaac gctgtgtgta aatacactta gcgccgggca ggagccgctt tcaaaccttt
    22561 ttggcggcaa aacgcccatg gaacaccgct ttgccgccgc ccgctggcag actggtgtga
    22621 ccggatgccc gcaactggaa gaggcactgg tttcgtttga ctgccgtatc agccaggtgg
    22681 tgagcgtcgg cacccacgac attttgtttt gcgccatcga agcgattcat cgtcacgcca
    22741 caccctacgg gctggtgtgg ttcgatcgca gttatcacgc gctgatgcgc cctgcttgtt
    22801 aacctcctta aggagacagc tgatggcaat gttcggtttt cctcactggc agttaaaatc
    22861 gacctctaca gagagcggcg tggtcgcgcc ggatgaacga ttgccgtttg ctcaaacggc
    22921 agtaatgggc gttcaacacg cggtggcgat gtttggcgcg acggtgttaa tgccaattct
    22981 gatggggctg gatcccaatc tttccatttt aatgtcgggg atcggcacgt tactgttttt
    23041 ctttattacc ggcgggcacg tgcccagtta tctcggttcc agcgcggctt ttgtcggcgt
    23101 ggtgattgcc gcgacgggct ttaatggtca gggaattaat ccgaatatca gcatcgcgct
    23161 gggcgggatt atcgcctgtg ggctggttta taccgtcatt ggcctggtgg tgatgaaaat
    23221 cggtacgcgc tggattgaac gactaatgcc accggttgtg acaggtgcgg tagtgatggc
    23281 gattggcctg aacttagcgc ctatcgcagt gaaaaatgta tcggcttcgg cctttgatag
    23341 ctggatggcg gtaatgacgg tgctgtgtat tgggctggtg gccgtattta cccgtggcat
    23401 gatccagcga ttattgattc tcgtaggatt aattgtcgcc tgtctgctgt atggcgtgat
    23461 gaccaacgtt ttagggctgg gaaaagccgt cgattttact ctcgtcagcc atgctgcctg
    23521 gttcggtctg ccacattttt ccactcctgc ttttaatggt caggcaatga tgctgattgc
    23581 gcccgtggcg gtgattctgg tggcggaaaa cttaggtcat ctcaaggcgg tcgccgggat
    23641 gaccggacgc aatatggacc cgtatatggg gcgggcattc gtaggcgatg ggctggcgac
    23701 gatgctttct ggctctgtcg gcggtagcgg cgtaaccacc tacgccgaga atattggtgt
    23761 gatggccgtg acaaaagtct actcgacgct ggtgtttgtt gccgctgccg tcatcgccat
    23821 gctgttgggc ttttcgccga aatttggcgc gctgatccat actattccgg ccgcggtgat
    23881 tggtggtgca tcaattgttg tgttcgggct gattgccgtc gcaggcgcac ggatctgggt
    23941 acaaaacagt gtggatttaa gccagaacgg taatttgatt atggtcgcag taaccttagt
    24001 gctgggtgcg ggtgattttg ctctcacact gggcggtttt acgttgggag ggatcggtac
    24061 agccaccttt ggcgcgattt tactcaatgc gttgttaagc agaaggttgg ttgacgttcc
    24121 accgccagaa gttgttcatc aggagccata aatagaaaac cgccggggta accggcggtg
    24181 atgacttttt gacgagtttc agacaaaaat acattcgtca tggattagcc cggctagttg
    24241 tcgcttttac cgtgactgct cttgcctcct tttttacctg cttcagatgc gcgctgcgga
    24301 tcatttttga aattaccccc gctgtgctgt ccaccttttt tacctgcttc tgatgctctt
    24361 tcgcggtctt ctgcaaaatt gccggaaccg cctcgatggt ttgccattac tgacctccgt
    24421 ctgggtatag acatcatatt gcatagaatt aacgcggcgt atggcggctg ccatattttc
    24481 gctacgtggg actaagctta gtgaactgac gcgggcagac ccgcgtttag taatatcctg
    24541 caacagcttc ttagcgagaa tagatatcat ctccaataat tatccataag ccgctcttat
    24601 gattaaaaaa acgcgatgag ataaaaatgt gtcattttgc gacaaaatta cgtgcttgtg
    24661 aaagttgtta taaatcaaat aagtggttgt gaaatttgca ctctgaaaag gacgtcttat
    24721 ctttaaataa gtggtagcga atcgctacgg aatagagata acacgaggag tggttagaaa
    24781 tggctaaagt tctggtgctt tattattcca tgtacggaca tattgaaacg atggcacgcg
    24841 cagttgctga gggtgcaagc aaagtggatg gcgcagaagt tgtcgttaag cgtgtaccgg
    24901 aaaccatgcc gccgcaatta tttgaaaaag caggcggtaa aacgcaaact gcaccggttg
    24961 caaccccgca agaactggcc gattacgacg ccattatttt tggtacacct acccgctttg
    25021 gtaacatgtc cggtcaaatg cgtaccttcc tcgaccagac gggcggcctg tgggcttccg
    25081 gcgcactata cggaaaactg gcgagcgtct ttagttccac cggtactggc ggcggtcagg
    25141 aacaaaccat cacatccacc tggacgaccc ttgcgcatca cggcatggtg attgtcccca
    25201 ttggctacgc agcgcaggaa ttatttgacg tttcacaggt tcgcggcggt acgccgtacg
    25261 gcgcaaccac catcgcaggc ggtgacggtt cacgccagcc cagccaggaa gaactgtcta
    25321 ttgctcgtta tcaaggggaa tatgtcgcag gtctggcagt taaacttaac agctaatctt
    25381 caacaggagg atacgcatgc caactcaaga agcgaaagct catcacgtcg gtgaatgggc
    25441 atctctgcgc aatacgtcgc cggaaatagc cgaggccatt ttcgaagtcg ccgggtacga
    25501 cgaaaaaatg gcggaaaaga tttgggaaga aggtagcgat gaagtgttag tcaaagcctt
    25561 tgctaagacc gataaagatt cgcttttttg gggcgaacag accatcgaac gtaaaaacgt
    25621 ttaaccagcg actcccccgc ttcacgcggg ggagttttct gttatttcac cgcttcattc
    25681 aatacgcgat caaacttatc cattgggcaa aaaccgttgg catctatcgg gcaaccgctt
    25741 aattccagcg tcacgcgctg cgcaggtgcc tgcagggtta acgcatcggc attacgtaac
    25801 tgttccgcac tctggtacac atattcaatt ttcatcaaat cgcgattggc tttgctgtca
    25861 tgccaacgct ggaaaacgat tttgccgcca atcggcgtgc gttcgttctg gtcatgcaac
    25921 tgatacggtt tgaaatccag cgccgttaac agagaggcaa tgttggagtc gtgcccaact
    25981 aataccgtga ttttcggtgc actggtgcga tcggtgacca gggctttgtc gatataactg
    26041 accagcggtt tcgcaacatt gcgcgccact tccggtgagg taaacaggct gtcctggtaa
    26101 ccgtttttca gcttcgacaa caccttccac tgctggtcag atttgatttc tccccaggcc
    26161 acctgatcca tcggaaaacc ttcgtagtat tgcaaagtaa acgcatctac cagcgagttg
    26221 ccgactttca gcggcccgga aacgcccggt tcttgttgat acttcgcgct aaaggtattt
    26281 ttgccatcta caagcgaaca ttgttgcttc tctttacacg caggagaatc tttatagtta
    26341 acgatttgtt ccagtagctt gtagctgtcg gtaagctgga gtttgctgag ctctttctcc
    26401 attgccgcca ccgcctgttc actgaatgcg gcggaatcat cggtgatcac tgggttaaag
    26461 gtggggtcca tagtgcccat tttttcctgg tgatgaacag gaatatcaca cccagggaat
    26521 gcgccagtaa taaagaactg cgcggtcgcg acggtgcgtt gcagactatt ggcataggca
    26581 taaacggtgt ccggtggcgg gcattcccct gatttcacca tcccctgttc tgccagccat
    26641 tcacgcatat aatggcccat atacacttcg agcacgcccc ctttagtggt gagctgccca
    26701 ccggggacgt cccattcagg ccatttattc ggcgtcgact gctccagcac actgccattg
    26761 ttcgccagcg gcgcacgtaa gttatgacgg ctcatcataa ggacttgctg tagctgatag
    26821 ccttccggta cggtttgtgc ctgagcattt gaagcgagta aaactatccc tgccacagtt
    26881 gcggcgatta gcgttttgtt cattcctcac acctcttttg ttatcaataa ttaaagagtg
    26941 tgacagaaat atgtcatttt tccgggaaca ggttcgcaaa atggctcgtt gaacgcctgt
    27001 ttt
//
LOCUS       DAZGAP010000059        26739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGAP010000059 DAZGAP010000000
VERSION     DAZGAP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.059"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..1835
                     /gene="oppA"
                     /locus_tag="ACHLAM_002057"
     CDS             204..1835
                     /gene="oppA"
                     /locus_tag="ACHLAM_002057"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO7853584.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1921..2841
                     /gene="oppB"
                     /locus_tag="ACHLAM_002058"
     CDS             1921..2841
                     /gene="oppB"
                     /locus_tag="ACHLAM_002058"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO7853585.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2856..3764
                     /gene="oppC"
                     /locus_tag="ACHLAM_002059"
     CDS             2856..3764
                     /gene="oppC"
                     /locus_tag="ACHLAM_002059"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO7853586.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3776..4789
                     /gene="oppD"
                     /locus_tag="ACHLAM_002060"
     CDS             3776..4789
                     /gene="oppD"
                     /locus_tag="ACHLAM_002060"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO7853587.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4786..5790
                     /gene="oppF"
                     /locus_tag="ACHLAM_002061"
     CDS             4786..5790
                     /gene="oppF"
                     /locus_tag="ACHLAM_002061"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO7853588.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5843..6172)
                     /gene="yciU"
                     /locus_tag="ACHLAM_002062"
     CDS             complement(5843..6172)
                     /gene="yciU"
                     /locus_tag="ACHLAM_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO7853589.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6207..7667)
                     /gene="cls"
                     /locus_tag="ACHLAM_002063"
     CDS             complement(6207..7667)
                     /gene="cls"
                     /locus_tag="ACHLAM_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO7853590.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7810..7983
                     /gene="yciY"
                     /locus_tag="ACHLAM_002064"
     CDS             7810..7983
                     /gene="yciY"
                     /locus_tag="ACHLAM_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO7853591.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8038..9291)
                     /gene="kch"
                     /locus_tag="ACHLAM_002065"
     CDS             complement(8038..9291)
                     /gene="kch"
                     /locus_tag="ACHLAM_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO7853592.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9592..9888)
                     /gene="yciI"
                     /locus_tag="ACHLAM_002066"
     CDS             complement(9592..9888)
                     /gene="yciI"
                     /locus_tag="ACHLAM_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO7853593.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10112..10831
                     /gene="tonB"
                     /locus_tag="ACHLAM_002067"
     CDS             10112..10831
                     /gene="tonB"
                     /locus_tag="ACHLAM_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO7853594.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(10871..11269)
                     /gene="yciA"
                     /locus_tag="ACHLAM_002068"
     CDS             complement(10871..11269)
                     /gene="yciA"
                     /locus_tag="ACHLAM_002068"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO7853595.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11374..11913)
                     /gene="yciB"
                     /locus_tag="ACHLAM_002069"
     CDS             complement(11374..11913)
                     /gene="yciB"
                     /locus_tag="ACHLAM_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO7853596.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(11943..12686)
                     /gene="yciC"
                     /locus_tag="ACHLAM_002070"
     CDS             complement(11943..12686)
                     /gene="yciC"
                     /locus_tag="ACHLAM_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO7853597.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(12714..12803)
                     /locus_tag="ACHLAM_002071"
                     /pseudo
     CDS             complement(12714..12803)
                     /locus_tag="ACHLAM_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13043..13681
                     /gene="ompW"
                     /locus_tag="ACHLAM_002072"
     CDS             13043..13681
                     /gene="ompW"
                     /locus_tag="ACHLAM_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO7853598.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(13740..14246)
                     /gene="yciE"
                     /locus_tag="ACHLAM_002073"
     CDS             complement(13740..14246)
                     /gene="yciE"
                     /locus_tag="ACHLAM_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7853599.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14292..14792)
                     /gene="yciF"
                     /locus_tag="ACHLAM_002074"
     CDS             complement(14292..14792)
                     /gene="yciF"
                     /locus_tag="ACHLAM_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7853600.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETYGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(14878..15057)
                     /gene="yciG"
                     /locus_tag="ACHLAM_002075"
     CDS             complement(14878..15057)
                     /gene="yciG"
                     /locus_tag="ACHLAM_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7853601.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(15438..16244)
                     /gene="trpA"
                     /locus_tag="ACHLAM_002076"
     CDS             complement(15438..16244)
                     /gene="trpA"
                     /locus_tag="ACHLAM_002076"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO7853602.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNLAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(16244..17437)
                     /gene="trpB"
                     /locus_tag="ACHLAM_002077"
     CDS             complement(16244..17437)
                     /gene="trpB"
                     /locus_tag="ACHLAM_002077"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO7853603.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(17449..18810)
                     /gene="trpCF"
                     /locus_tag="ACHLAM_002078"
     CDS             complement(17449..18810)
                     /gene="trpCF"
                     /locus_tag="ACHLAM_002078"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO7853604.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPAHIATVYRNYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(18811..20406)
                     /gene="trpD"
                     /locus_tag="ACHLAM_002079"
     CDS             complement(18811..20406)
                     /gene="trpD"
                     /locus_tag="ACHLAM_002079"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO7853605.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHVPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(20406..21968)
                     /gene="trpE"
                     /locus_tag="ACHLAM_002080"
     CDS             complement(20406..21968)
                     /gene="trpE"
                     /locus_tag="ACHLAM_002080"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO7853606.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQSLLNVPNEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(21990..22083)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(22060..22104)
                     /gene="trpL"
                     /locus_tag="ACHLAM_002081"
     CDS             complement(22060..22104)
                     /gene="trpL"
                     /locus_tag="ACHLAM_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO7853607.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            22242..23123
                     /gene="rnm"
                     /locus_tag="ACHLAM_002082"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             22242..23123
                     /gene="rnm"
                     /locus_tag="ACHLAM_002082"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO7853608.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HYGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            23120..23740
                     /gene="yciO"
                     /locus_tag="ACHLAM_002083"
     CDS             23120..23740
                     /gene="yciO"
                     /locus_tag="ACHLAM_002083"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO7853609.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            23768..25657
                     /gene="yciQ"
                     /locus_tag="ACHLAM_002084"
     CDS             23768..25657
                     /gene="yciQ"
                     /locus_tag="ACHLAM_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO7853610.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVIRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAILREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            25870..>26739
                     /gene="rluB"
                     /locus_tag="ACHLAM_002085"
     CDS             25870..>26739
                     /gene="rluB"
                     /locus_tag="ACHLAM_002085"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO7853611.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHC
                     QVSGGRRSGGRNNN"
BASE COUNT         6751 a         6496 c         6763 g         6729 t
ORIGIN      
        1 taatgtgtct cgacagggga gacacagtac gaatcgacat aaggtgatcg tctgaatcac
       61 cagaataaat aaagtcggtg atagcaatcc gtaacgataa agtaacctga cagcagaaag
      121 tctccgagcc tgtgcagggt cccaatccgg gattacacat gctggttaat accagtaatt
      181 ataatgaggg agtccaaaaa acaatgacca acatcaccaa gagaagttta gtagcagctg
      241 gcgttctggc tgcactaatg gcagggaatg tcgcgctggc agctgatgta cccgcaggcg
      301 tcacactggc ggaaaaacaa acgctggtac gtaacaatgg ttcagaagtt cagtcattag
      361 atccgcataa aattgaaggt gttccggagt ctaatatcag ccgagacctg tttgaaggct
      421 tactggtcag cgatcttgac ggtcatccgg cacctggcgt cgctgaatcc tgggataata
      481 aagacgcgaa agtctggacc ttccatttgc gtaaagatgc gaaatggtct gatggcacgc
      541 cagtcacagc acaagacttt gtgtatagct ggcaacgttc tgttgatcca aacaccgctt
      601 ctccgtatgc cagttatctg caatatgggc atatcgccgg tattgatgaa attcttgaag
      661 ggaaaaaacc gattaccgat ctcggcgtga aagcaattga tgatcacaca ttagaagtca
      721 ccttaagtga acctgttccg tacttctata aattacttgt tcacccatca acttcaccgg
      781 tgccaaaagc cgctatcgag aaattcggcg aaaaatggac ccagcctggt aatatcatca
      841 ccaacggtgc ctatacctta aaagattggg tcgtaaacga acgaatcgtt cttgaacgca
      901 gcccaaccta ctggaacaac gcgaaaaccg ttattaacca ggtaacctat ttgcctattg
      961 cttctgaagt taccgatgtc aaccgctacc gtagtggtga aatcgacatg acgtataaca
     1021 acatgccgat cgaattgttc cagaagctga aaaaagagat cccggacgaa gttcacgttg
     1081 atccatacct gtgcacttat tattacgaaa ttaacaacca gaaaccgcca ttcaacgatg
     1141 tacgtgtgcg taccgcactg aaactgggta tggaccgcga tatcattgtt aataaagtga
     1201 aagcgcaggg cgacatgccc gcctatggtt acactccacc gtatactgat ggcgcaaaat
     1261 tgactcagcc ggaatggttt ggctggagcc aggaaaaacg taacgaagaa gcgaaaaaac
     1321 tgctggctga agcgggttat accgcagaca aaccgttgac catcaacctg ttgtataaca
     1381 cctccgatct gcataaaaag ctggcgattg ctgcctcttc attgtggaag aaaaacattg
     1441 gtgtaaacgt caaactggtt aaccaggagt ggaaaacgtt cctcgacacc cgtcaccagg
     1501 gcacttttga tgtggcccgt gcaggctggt gtgcagacta caacgaacca acttccttcc
     1561 tgaacaccat gctgtcgaac agctcaatga ataccgcgca ttataagagc ccggcctttg
     1621 acagcattat ggcggaaacg ctgaaagcga ctgacgaggc gcagcgcaca gctctgtaca
     1681 ctaaagcaga acaacagctg gataaggatt cggccattgt tcctgtttat tactacgtga
     1741 atgcgcgtct ggtgaaaccg tgggttggtg gctataccgg caaagatccg ctggataata
     1801 cctacacccg gaatatgtac attgtgaagc actaatggca atacgtgggg caggcgtgta
     1861 ctgctccacg gtgtctgatt tttatcgcat tacagaaggc acaggccaga aggtagggca
     1921 atgttaaaat ttattctacg tcgctgtctg gaagcgattc cgacgctatt tattcttatt
     1981 actatttcgt tcttcatgat gcgcctcgcg ccgggaagcc cttttaccgg cgaacgtact
     2041 ttaccgccag aagtgatggc caatatcgaa gcgaaatatc atcttaatga tccgatcatg
     2101 acacagtatt tcagctacct gaaacaactg gcgcatggcg atttcggtcc atcgtttaaa
     2161 tataaagatt attcggtcaa cgacctggtt gcatccagtt ttcccgtttc tgccaaactg
     2221 ggagccgcag catttttcct tgcggtaata ctgggtgtta gtgctggcgt tattgccgca
     2281 ttaaaacaaa acaccaaatg ggactatacc gtgatggggc tggcaatgac cggggttgtt
     2341 atccccagtt ttgtggttgc gccattatta gtcatgatat ttgcgatcat tttgcattgg
     2401 ctgccgggcg gtggctggaa tggtggtgcg cttaaattca tgatactgcc aatggtggcg
     2461 ttgtcactcg cctatatcgc cagtattgcg cgtattaccc gtggctctat gattgaagta
     2521 ttacactcca actttattcg tactgcccgg gcgaaagggt tacctatgcg gcggatcatt
     2581 ttacgccacg cattaaaacc tgctctgtta cccgtgctct cctatatggg ccccgcattt
     2641 gtcggcatta ttaccggttc tatggtcatc gaaaccattt atggtttgcc ggggattggg
     2701 caactgttcg ttaatggcgc attgaaccgt gactattcct tagtgttaag cctgactatc
     2761 ctggttggtg cgttaaccat tttgtttaat gccattgtcg atgtgctata tgcggttatc
     2821 gacccgaaaa tccgttactg atactggagc tcgcgatgat gttaagtaag aaaaacagcg
     2881 agacgctgga aaatttcagt gaaaagctgg aggtcgaagg gcgcagcttg tggcaggacg
     2941 cacgtcgacg ttttatgcat aaccgtgcgg cggttgccag tctgatagtg ctggtgctaa
     3001 tcgcgttatt tgtaatcctg gcaccgatgc tttcgcagtt tgcctatgac gatactgact
     3061 gggcgatgat gtccagcgcc ccggatatgg agtccggtca ctactttggt actgactcat
     3121 ccggtcgcga cctgcttgtg cgtgttgcga ttggcgggcg tatctcactc atggtcggtg
     3181 ttgctgcggc actggtggca gtggtcgtgg ggacacttta tggttcgctt tccggttatc
     3241 tgggcggtaa agtggattcg gtaatgatgc gtctgctgga aatcctcaac tccttcccat
     3301 tcatgttctt cgtcattttg ctggtgacct ttttcggtca aaacatcctg ctgattttcg
     3361 tggcgattgg catggtttcc tggctggata tggctcgtat tgtgcgtggg caaaccctga
     3421 gtctgaagcg caaagagttt attgaggcgg cacaagttgg cggtgtatcg acgtcgggca
     3481 ttgttattcg ccacattgtg ccgaatgtac tcggtgtggt ggtggtctac gcatcacttc
     3541 tggtgcccag catgatcctc tttgaatctt tccttagctt cctggggttg gggacgcaag
     3601 agccgttaag cagctggggc gcattgctga gtgatggcgc gaactcgatg gaagtctctc
     3661 catggttact gttgttccca gcgggattcc tcgtggtgac gctattttgt ttcaacttta
     3721 taggcgatgg cttgcgtgat gccctcgacc cgaaagatcg ttaaggagtg cagccatgag
     3781 cgtaattgaa actgcaactg tgccgctcgc acaacaacag gctgacgcac tgctgaacgt
     3841 gaaagatttg cgtgtcacct ttagtacccc ggacggcgac gtcacggcgg tcaatgattt
     3901 gaatttttcc ctacgtgccg gagaaacgct gggcattgta ggtgagtctg gttcgggtaa
     3961 atcgcaaact gcatttgcgt tgatgggcct gctggctgcc aacggacgta ttggcggatc
     4021 ggcaaccttc aatgggcgtg aaatcctcaa tttgccagag cgtgaactca atagactgcg
     4081 cgctgaacaa atctcaatga tttttcagga cccaatgact tcgttgaatc cctatatgcg
     4141 cgtcggtgag cagttgatgg aagtgctgat gctgcataag aacatgagca aagctgaggc
     4201 gtttgaagag tcggtgcgga tgctcgatgc ggtaaaaatg ccggaagcac gtaaacgcat
     4261 gaaaatgtac ccgcatgaat tttctggtgg catgcgtcag cgagtcatga ttgcgatggc
     4321 attgctatgt cgacctaagc tgctgattgc ggatgaaccc actactgcgc tggacgtcac
     4381 cgtacaggcg cagatcatga cgctattaaa tgaactgaag cgggaattta ataccgccat
     4441 cattatgatc acccacgatc ttggcgtggt ggcggggatc tgcgacaaag tgctggtaat
     4501 gtacgccggg cgcacgatgg aatatggcaa cgcgcgcgat gtcttttatc aacccgttca
     4561 tccttattct atcggtttgc tcaacgcggt gccgcgtctc gatgcggaag gtgaaacaat
     4621 gttgaccatc cctggtaatc cgccaaacct gctgcgatta ccgaaaggtt gcccgttcca
     4681 gccacgttgt ccgcatgcga tggaaatttg tagtagcgca ccgccgctgg aagagtttac
     4741 gcctggccgt ctgcgtgctt gctttaaacc ggtggaggaa ctgttatgaa tgctgtaacc
     4801 gaaggaagaa aagtcctcct cgaaatcgcc gatctgaaag tgcactttga aatcaaagat
     4861 ggcaaacagt ggttctggca accgccgaaa acgctcaaag ccgtcgatgg tgtcactctt
     4921 cgcctgtatg aaggggaaac attaggtgtg gtaggggaat cgggatgcgg taagtccacc
     4981 tttgctcgcg ccatcattgg tttggtcaag gcgaccgacg gtcatgttgc ctggttaggt
     5041 aaagagttgc tgggcatgaa gcccgatgaa tggcgtgccg ttcgcagtga tattcagatg
     5101 attttccagg atccattggc atcgcttaac ccgcgtatga ccatcggcga gatcatcgct
     5161 gaaccactgc gtacttatca tccgaaaatg tcacgccagg aagttcgcga gcgcgtgaag
     5221 gcgatgatgc tgaaagtcgg gttattgcct aacctgatta accgctatcc gcatgagttc
     5281 tctggtgggc agtgccagcg tatcgggatt gctcgtgctc ttattcttga accgaagctg
     5341 attatctgtg atgagccggt gtcggcgctg gacgtgtcaa ttcaggcgca ggtggtcaac
     5401 ctgctccagc agctgcaacg cgagatggga ttgtcattaa tttttatcgc tcatgacctg
     5461 gccgtggtaa aacacatttc cgatcgtgtg ctggtgatgt atctcggcca tgcggtagaa
     5521 ctggggacct atgatgaggt ctatcacaat ccgctacatc cttacaccag ggcattgatg
     5581 tcggcagtcc ccatacctga tccggatctg gagaagaaca aaaccatcca gttactggaa
     5641 ggggaattac cgtcgccgat caacccgcct tccggttgtg ttttccgtac ccgttgcccg
     5701 attgccggtc cagagtgtgc caaaacacgt cctgtgctgg aggggagttt cagacacgcc
     5761 gtttcttgcc tgaaagtcga tccgctttaa aagcaataag ggctgacagt tgtcagccct
     5821 tattcacgcc aaaagcgatt atttattccc gccagatgat atggcacagt ttgtgatctt
     5881 tttcgcggca taacagaatg cgcgcgaaaa catcgttgat ttcaccatct tcactgtccg
     5941 ccagaccaat caccacttcg gcaaaaaagt ccgggttcaa atcgaaatca acatgttcct
     6001 gccagtcttc tgccggatcg aataactccg cgccgccgcg ctcttcaaac tgaagattga
     6061 acagcagaac atcagctgga tcgagattgt cggcagccag ttcgagaaaa atatcgtagg
     6121 cctgctcaag cgtttcatct tcagtcaggc gattgtttag atccatatcc atagtcacta
     6181 cctgtttaac ctctgttggc gacgttttac agcaacgggc tgaagaagta aaacagtcgc
     6241 tcggcgacac gttgccataa tggacgtttt agccataaac gggcatcgag cagacgtgaa
     6301 cgcgaaatat aatcgtcctg aacggcggca aggtctgcac caaaaccttt atcgtcgatt
     6361 gccagggtaa tctcgaaatt tagccacaga ctccgcatat caaggttaac ggtgccaacc
     6421 agacttagtt caccatcgac cagcacgctc ttggtatgca gtaacccgcc ttcaaactga
     6481 taaattttaa ccccagcagc cagcagttcc gtaaagaatg cgcgactggc ccagccgacc
     6541 agcatcgagt cattttttcg cggaaggata atactgacat ccaccccgcg ctgcgccgcc
     6601 gtgcaaatcg catgaagtaa atcatcgctg ggcacaaagt agggcgttgt catgatcaaa
     6661 tattcacgcg ccgaataagc agcagtcaat aatgcctggt gaatgagatc ttccggaaag
     6721 ccggggccag aagcaattgt gtgaatggtg tgaccgctgg cctgctcaaa cggcataata
     6781 ttgacatctg gtggtggcgg cagaatacgt tttccggttt caatctccca gtcgcaggaa
     6841 taaataatcc ccatcgcggt agcgatggga ccttccatac gcgccatcag atcaatccat
     6901 tgccctacgc ccgcatcttg tttgaagtag cgagggtcga ccatattcat gctgccggtg
     6961 tacgcgatgt aattatcgat catgatcatc ttgcgatgtt ggcgcaggtc catacggcgt
     7021 aaaaacacac gcatcagatt gacctttaag gcttcgacaa cttcaatacc ggcattacgc
     7081 attagctcgg gccacgggct gcggaaaaaa gccacactcc cggcggagtc gagcatcaat
     7141 cggcaatgaa tgccgcgtcg cgcagccgcc attaatgatt cagccacctg gtccgccatg
     7201 ccgccgggct gccagatata aaacaccatc tcaatattat ggcgagcgag ctggatgtcg
     7261 cggattaacg cctgcatcac atcatctgac tcggtcatca gttgcagctg attccctttg
     7321 accccagcga tcccctgacg acgctcgcaa agcttgaata atggcgcagc gacactgcta
     7381 ttttcttcgg cgaagatatg cttacaggct ttaaggtcgt taagccattt tgcggtggaa
     7441 ggccacatcg ctctggcgcg ctcagcgcgg cgtttgccta aatggagctc gccaacggca
     7501 agataggcaa taattccgac taacggcaga atgtaaataa tcaacagcca ggccatcgcg
     7561 gagggaactg cgcgtcgttt cattagaatg cgtaaagtta cgcctgcaat gagcaaccag
     7621 tatcccagaa tggccaacca actcaccaac gtataaacgg ttgtcataga ttagaaatcc
     7681 ttttgaaagc gcattgttat gagtttacgc atatggattc atctggcaaa taaaaacgcg
     7741 ggaaaagcac tggtcagcgt cgggggcggg ggtataatga ccattctgtt attgcataga
     7801 gtagttaaca tgaagcggag tagaacggaa gtggggcgct ggcgcatgca gcgtcaggct
     7861 agccgacgta aatcgcgttg gcttgagggg cagtcgcgcc gaaatatgcg tatccacagc
     7921 atcaggaagt gcattctaaa caaacagcgt aactcgttat tgtttgcgat ctacaatatc
     7981 taaatgtact gggcaccatt gcggtgccca ataagtttac tcttccctct ttaaactcta
     8041 tttttgcgcc gattctttac tgtcagcttt cgtttcttgt tcatcgttat cgctgaaaat
     8101 gccatgaccg gagttcaaca acattgaaac gagcatatca ttattaatct cttcaccatt
     8161 taaaactcgc gccagaattt cgctgccaaa cagttgcggc gagagaatga tatccggatg
     8221 taccatctta atcttattca ggtttttgct atcactgacg gcgagaactg ttttgacatc
     8281 actgctcata tcttttgccg agagtacaac aaacgcgtta tctgcatcgt tatcactcag
     8341 cgccagaatg gcccggcatc gatcaattcc agctttcttt aatactgaac tgtcattact
     8401 gtcaccgggg ataacatcag cgttatcgcc taagcgttgc tcaagttgct tgatatcatc
     8461 ttcaggcaag ttgctgataa ccgttacgtt ttgtccgcgt tgattcagtt gcaggatcgt
     8521 attgatggcg agaatcgaat gtccgcaaac aataaaatga tctttacgat gcattgtatg
     8581 attgtttcct tttacaagtt tgttgaatcc cccgcggata agcggaccaa aaattgaggt
     8641 catagatgtg gcaaaaacgg taatgccgga aataatgacc gaaatagtga acaatcgtgc
     8701 tgattcagaa acagggacaa tatcgccata gccgacggtt gacatggttt ctatcgaaaa
     8761 atagaacgcg gtcatcaaac tttctattcg cggattaaaa ccttcgctta aataaagcgc
     8821 accgtaggtg gagtaaaaca gtaacgtcgt gaaactaata aatgcgaaaa ttgtcccggc
     8881 tgcggcgcta ctgtgggaga agtctttgcg cagtatcagc aaaaacacca gcgtaaaaat
     8941 gcaaaatcca atactaaatt tcagccaggg ataaaaatgc agggtgtaaa ttagcgctat
     9001 caacaacaaa atgatactga ttgcccatgc gagctttgcc cggaacagta agccaatgga
     9061 gttcaggaca agaaagacgc cgagcataaa gagtggggcg tttgccagca aggagagatc
     9121 caactctgaa aatgctttga tatggaaaat atctagcaaa ttgaccgaca taccatagat
     9181 tgttttaaaa ataagcaatc catttaaaaa gacggccagc gcgagaatgt cgtgccgtag
     9241 ggtcacccat aaattagttg ctgtttgttt gaatgtagcc cagtgactca ctgatacctc
     9301 tgtaaccaca attttgttta attatcttag cgtgcacatt ttctgaacat acatgcagcg
     9361 cgtgaatgtg ttaaacaata gtagatgact ttatccgcag taatcaaagg ttttgagtga
     9421 gcaactctgc gtcaggaaag ggcatttttc ctgaatatgc tcacatcata taaagaaata
     9481 cagataaagt tattatctgc ttgtggtggt aaatgcaatg actgacctat aaggaaaggc
     9541 caaacaagaa cacggttgct aaaaccgtgc ccttaaatat tgaatttcta ttcagaacac
     9601 tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt acgggtcagc
     9661 atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg ttgagccagt
     9721 aaatcccgca gcccctggat cgttactgtc tactgctggc attggaccgg cggtcaacaa
     9781 tcgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa cggaaaggcg
     9841 tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg gcacaactcc
     9901 ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca acggaaagat
     9961 gatgtctttg ttaagaccat ttaaaaagta aggtcaatta cgccaaaaat gacattttca
    10021 ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa aatcgaggcc
    10081 tggtttttct actgaaatga ttatgacttc aatgaccctt gatttacctc gccgcttccc
    10141 ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcgggtc tgctctatac
    10201 ctcggtacat caggttattg aactacctgc gcctgcgcag ccaatttctg tcacgatggt
    10261 tgcgcctgcg gatctcgaac cgccacaagc cgttcagccg ccaccggagc cggtggtaga
    10321 gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg tggtcattga
    10381 aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac aggagcagcc
    10441 aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa atactgcacc
    10501 cgcgcgccct acatcaagta cggcaacggc tgcaaccagc aagccggtta ccagtgtggc
    10561 ttcaggacca cgcgcattaa gccgcaatca gccgcagtat ccggcacgag cacaggcatt
    10621 gcgcattgaa gggcaggtta aagttaaatt tgatgtcacg ccggatggtc gcgtggataa
    10681 cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga aaaatgcgat
    10741 gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga acatcctgtt
    10801 taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc cgaccggagg
    10861 cttttgatta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca accgcgacat
    10921 acttaaataa tgcttctgtc gctttatagc gttgcccgat gggttcagac gcgacttttt
    10981 tcacccacac ttcaatatta atgctaaccg atgtcgtccc tttctggaca cagcgtgcat
    11041 agcagcacac cacatcgccg accgcaaccg gtcgtaagaa agtcattcct tcaacccgca
    11101 cggtcactac gcgaccgtgg gcaatttctt tcgccagaat agcgccgcca atatccattt
    11161 gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg ggcatggcta
    11221 aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg gtaaaaccga
    11281 cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga acagaggaga
    11341 tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct gcggcatgtg
    11401 gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg tcaggccaaa
    11461 gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga tgtttgccag
    11521 accgcaaagg ataaagaaaa cagcccaggc cagattcagc ttcgaccata ccggttgcgg
    11581 cagcgtgagt tctttaccca gcatccgctg aattagcggc tttttcatca cccattggct
    11641 gactaacagg gcacccgcaa acagggcata aatgacagta accttccatt taataaactc
    11701 atcattgtgg aagaacaggg tcaagccacc gaagacgacc accagaacaa aagtgatcag
    11761 ggccatcttc tcaaccttac gaaagcgaac ccagctatat ataagcacaa tcgccgtggc
    11821 gacgatcagc gccgcagtag ccgcatagat gtcataaatc ttgtaaaacg cgaaaaagac
    11881 aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc aaatgcttaa
    11941 ggttattggc gaatcaacat atacaggcgg aacagataga tgagcagtac ggctgaaatc
    12001 aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc ggcaaaagaa
    12061 gaggcaaaaa gcagcaacag tgtctttgcc agcaaccagc tcagtactgc gggtgccacc
    12121 agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa aacgcccatt
    12181 ttgtcctgta ccaacatcac cggtgccagg gccagtaaaa tggccatgat aattcccgga
    12241 acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat cagaataaat
    12301 aactttggca atatcggcgc actggcacca atagctcgca gcgcactgac tctctgaccc
    12361 gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt accgattaat
    12421 cctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc cggtgacata
    12481 ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc attgagctgc
    12541 gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat aaacgcacat
    12601 agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc ggtgtcacgg
    12661 tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag gtgttaatta
    12721 gcgggtaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt atggaaaagc
    12781 atatctttgt aaagcggagg taattgctgg ccttcagacg tgggcaaatc gcggcaagtg
    12841 gcgatcgagt taatatttgc gtagcgaaaa tatttaaaaa ttgatttaaa tcacattaac
    12901 caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa ttatcatatg
    12961 taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt cacgttttta
    13021 taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg cagtaacaac
    13081 tcttctctct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc gtgcaggttc
    13141 tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg gtggattcag
    13201 cgtgaccaat aatacgcaac tgggcctgac gtttacttat atggcgaccg ataacattgg
    13261 tgtggaattg ctggcagcga cgccgttccg ccataaaatc ggcacccggg cgaccggcga
    13321 tattgcaacc gttcatcatc tgccgccaac actgatggcg cagtggtatt ttggtgatgc
    13381 cagcagcaaa ttccgtcctt acgttggggc aggtattaac tacaccacct tctttgataa
    13441 tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga aagattcctg
    13501 gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt tggttaacat
    13561 gtcagtctgg tacatggata ttgataccac tgccaattat aaactgggcg gtgcacagca
    13621 acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat atcgttttta
    13681 attccgcaca aaaatgaccc cgtaattacg gggtcaataa ggacatggta taaagggtat
    13741 tatttcttcg cttctacgcc atcagtttca gagcgaatta agaatttctc agttgtttgc
    13801 ggaatatgct gaattagcca gtcggccatg tgcttttcat catttaaaat cgcttcgata
    13861 atcggaattg aggctgtatc accggcattt tttgctgctg ctaagagtga ggtataacag
    13921 gcgatttcaa attgctcgaa gacatatccg ctaatagagc ctttgactat ttcatcagaa
    13981 gggaatatac caccgattga ctgcccaagt gcagccattt tactcatgga atctttaatg
    14041 actgaacgtg aaatgtcatt acgatcaaga atagtttcca gttgaacaat ctggtttttg
    14101 gtttcactaa gatgttgttc aatacgagcg cgtagttcag gataattatc tatacgactg
    14161 gccatggatt caagcattga ttcggcttgc ttttccattg cgtgggcgtc acgtaaccag
    14221 tcatgataat gttcaatacg attcatgaaa atgtcctctt tataaaatga ctaaaagtta
    14281 aagttcatat ttcaggcttt attttcggct ttcttattta cgttattaag ggccagatca
    14341 gtcaatttga tgtcggtggc cttttcttct tccagggtct ctttcagaag cttcgctgct
    14401 ttacgataac ctaattgttc agccagcgtc gctaatgtcc cgtaactggc aatctcataa
    14461 tgctcgactt tctgtgctgc ggcaatcagt gcggcatcac gcacttcgtt tttctcggta
    14521 ctttcgatga cctcattagc ttcttcaata agaccttcca ttgccacaca tttcatgcgc
    14581 ttaattttca gattcgattc tgattccaca acttgatcaa tacgttcaat ctgtccatat
    14641 gtttcctcga ggtgcgcatg aaaagcctga cttaattttt cgtttgatgt tgctcttgcg
    14701 agttttgcca gtgcccgggt taattgtttt tctgcgctgt aggtatctga aagcaggtga
    14761 ataaatacat cttcaatggt cttcatattc atatttttct ccagtgaaat cactgcgagc
    14821 catacggccc gcagatgcaa ttatctttat cagctgatat taataattaa cggtgaatca
    14881 ggatttgcct gatttattac caccgctttg ttgaccgcct tttttacccg cttcagatgc
    14941 gcgttgcgga tcatttttaa aattaccgcc gctatgctga ccgcctttac ggccagcgtc
    15001 ggatgccttt tcacggtctt cggcgaaatt tcctgaacca ccacgatgtt cggccatgtt
    15061 atttctcccg ttgcgttgca ttgtttcatt aatatgggtg ttgtgtgtcg acactcatta
    15121 aaattagtcg ctaatgaaaa ttagtcaaat taagcgcaac gagaaaaaag agggaaaata
    15181 tattatgagg aacattctgg atatattaac aattgcctga ggaataagtg acttagagaa
    15241 aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat attactgttg
    15301 ggcggaaaaa agacgtaagt tgacgttcga caggggcaag cgaaacggta aaaagataaa
    15361 taataaatga atttaggatt tttccagctt cgttaaagaa agttaaaatg ccgccagcgg
    15421 aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac aaaagctttc
    15481 agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt aacgatggcc
    15541 gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg atccggggcg
    15601 gaaataccaa agccctgcaa tggaggcgca gcattgtatt ctttcagctt cgtgaccaga
    15661 tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgcacg tgacaataaa
    15721 taggtgtatc cacgaccgta ggaggctatc tggcgcagca aatcatcgtc ggcattcggc
    15781 gggcagataa agataggtgc aagattatga cgcaacgcgg cctggcggaa gggagcggac
    15841 tcttccactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc gcactgggca
    15901 taaaactcat caatgccttt gttaaacacc aggttggcat acatcagcag gccaatggga
    15961 atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc cggagtcaca
    16021 cctgccgcaa aggcgcgcag agtggcgttt tgaatcgtcg ggccatccgc cagtgggtcg
    16081 gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt gtcgataatt
    16141 ttcagcgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa tgcgccttct
    16201 ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt tcccctcgtg
    16261 ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa aggttaacca
    16321 ccagtagctg ctctttttcc gggttttcgc gcatcatttt cagcgcatgg gccagggcgt
    16381 gtgaggactc cagcgccggg ataatccctt cgtgcaggca gagcgttttg aaggcttcca
    16441 gtgcttcatc atcggtaatt gacacgtaat cagcgcgtcc agtgctgtta agatacgcgt
    16501 gttgtgggcc gacagacggg aaatccagtc cggcggagat ggagtaagat tcttcaatct
    16561 gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa atagatgccc acgcgaccgt
    16621 gttttaacgg tgcgccgtgc tcgccagttt cgataccgtg accacctggc tccacaccaa
    16681 tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca ttcgaaccgc
    16741 cgccaacaca ggcgataaca gcatccggca ggcgaccttc tctttccaga atctgcgctt
    16801 tcgtttcttc gccaatcatc cgctgaaact cacgcacaat ggtcgggtaa ggatgcgggc
    16861 cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag tcgcgtagcg
    16921 cctcattaca ggcatctttc agcgtcgcgg aaccgctatg caccgggatc acttccgcac
    16981 ccattaaacg catacgaaaa acgttaggcg actggcgttc aacgtctttg gcacccatat
    17041 aaatgcgaca tttcaggccg agcagggcgc tggcaagggc cgacgccacg ccatgctgac
    17101 cggctccggt ttcggcgatg atttccgttt tacccatccg cttcgccagc aacgcctggc
    17161 cgagcacttg gttggtttta tgcgcgccgc cgtgcagcaa atcttcgcgc ttgagataca
    17221 gcgtggtgtt agtgccagcc gtaatgttct ggcatttggt gagtgcggtt ggacgcccgg
    17281 catagttttt cagcaggtcg ttgaactgag cctgaaattc aggatctttt tgcgcgctaa
    17341 caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg tacatgccgc
    17401 caaactcacc aaaatacggg ttaagtaatg ttgtcattgt tcctttcctt aatatgcgcg
    17461 cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt gcgactctac
    17521 agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc agttatctgc
    17581 gcctaagccc cccgccagca gaacgttgcc aagcgattga ccgtttaaca gtgaccagtc
    17641 gaaacgttga ccgcttccac cctggccgtt gtctaaaaca tatttatcaa cgtgctgaag
    17701 ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa cgtgtgctgg
    17761 cagagcttca cgcagcgtat cgatatacag ctgatcttca ttaccatgca gttgcactgc
    17821 cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat tgcggaacac
    17881 gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa cgttgacgca
    17941 acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt cataagctgc
    18001 tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca atacccggcg
    18061 cacggcggcg ttcaaatcgt catgggccat caacgccgaa ccaatcagaa aaccgttagc
    18121 gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc tgattaccgt
    18181 cacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa tcgacaaatc
    18241 acgcagatcg cggttgttga tgccaacgac ctttgccccc aatgcaatgg cgcgctccag
    18301 ttcctcttca ttactgactt cggtcagcac acccatctcc agactgtggg cgacggcggc
    18361 aaggtggcga tattgttcgt catccagtac tgaaagcatt aataagcagg catcggcctg
    18421 gtaatagcgc gccagataga tctggtaagg gtcgataata aagtctttac ataaaatcgg
    18481 ctgcggggcg atttggctga cgatggagag gaaatcaaag ctcccctgaa aatatttctc
    18541 atcagtcagc accgaaattg ccgaagcgta atttctgtag acggttgcaa tgtgtgccgg
    18601 atcaaaatca tcgcggatca cgccttttga cggtgacgct tttttacact ccagaataaa
    18661 cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgc gtgctcggct gaacctcatt
    18721 ctgaaaactg gccagcggtt gctgctgttt gcgggcttct acccaaatcg ccttgtctgc
    18781 gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagtg cggtgactct
    18841 gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg cttgcagatc
    18901 ttcatggcca tgcaggcgca ttaacatggc gacgttcgcc gcgacggctg cttcatgggc
    18961 ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt ccggtgttcc
    19021 gcctgccagt tgctcctggt ggtagggtgt caggccaaaa tcttctgcag ttagctgata
    19081 gctcttaatt tcaccgtcat gcagttcggc aacgattgtc ggcgcgtgta atgaaacttc
    19141 atccatcccg ccgctgtgca ctaccgcagc gcgttgatac cccagcacgc gcaaggtttc
    19201 ggcaatcggc agcaccagtt ccggactata aacaccaatt agcgccagcg gcggatgcgc
    19261 cgggttaatc aatggcccca acacgttgaa cagggtgcgg gttttcagtt gctggcgaac
    19321 cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac agacgcctaa
    19381 ctcatccagc gcctggcgcg atttatcggc gttcatatca agattaatac cgaacgccgc
    19441 cagcagatcg gacgagccgg atttactgga gacgctgcgg ttaccgtgtt tcgccacttt
    19501 cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatgc tgttgctgcc
    19561 gtcaccgcca gtaccgacga tatcggcaaa cagataatcc gggcgcggga acggcgcggc
    19621 gttttccagt agcgcggttg ctgctccggc gatctcgttc gggtgctcgc cgcgaatttt
    19681 catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca ccaccgctga
    19741 aaacagctgg tgactttctt gttggctaag cgtctgtgcc tgatacagtt tttccagaat
    19801 cggttgcagc gtgttggttg gctcaagttt ctgctgcgcc caggccagcg tttgttccag
    19861 caggcgagcg ccctgggtag taagaatgga ttccggatgg aactggaaac cgcaaacgcg
    19921 atctgcatcg tgacgcaccg ccatcaccat gccattaaaa tgggcgttga tggttaaacc
    19981 tgccgggata ttactgccaa ccagcgagtg ataacgcgcc accggcaatg ggtttgttaa
    20041 tccggcaaac atcgcctgac tgtcatgttc aatgctcgac gctttaccgt gaagaatttc
    20101 gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc cgaggcaaat
    20161 gccaataatt ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac aaccggcttc
    20221 gctcggcaca ccggggccag gagaaagcat cagcaccgga ttgctcatcg tcgccaggcg
    20281 ttcaattaag gtctgcgccg gaacatggtt gcggtaaatc accacgttat gaccattgct
    20341 gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca gcagaatgtc
    20401 agccatcaga acgtctcctg tgcatgatgc gcggtggcaa tagcgcgcag tacagcgcgg
    20461 gctttattac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac tacgccagca
    20521 ccggcttgca cggtggcgat accgttttcc accagcgccg agcggatcac aatgcaggtg
    20581 tcgagatcgc catgcgcggt aaaataacct accgcgccgc cgtagctgcc gcgtcgacga
    20641 ccttctgctt cggcaattaa ctgcatagcg cgcactttcg gcgcaccgct taatgtcccc
    20701 atattcatac aggcgcggta agcgtgcagg gcgtcgagat cgtggcgcag ctcaccgacc
    20761 acgcgggaga cgaggtgcat cacataagaa taacggtcaa ctttggtgag atcggcgacg
    20821 tagcgactac cgggagtgca aatacgcgcc aggtcattac gggcgagatc cactagcatc
    20881 agatgttcag aaagctcttt atgatcggta cgcatttcca gttcgatgcg gctgtcgagg
    20941 tctctgtcca gtgaaccatc ggcacgacga ccgcgtggac gtgttccggc aatcgggtag
    21001 atctcaatct ggcggctggt ggcgtcatac ttgagcgaac tttccggcga cgcgccaaac
    21061 agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact ctttttcagc
    21121 acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga tggcaccacc
    21181 tggaaaattt ctccggcgcg aatcgctttt tgcagcgaac gcactacgcc accgaactct
    21241 tcatcactct ggttacattc acaacgcata tgcggcacgg aaaccaccgg cagcggcggc
    21301 gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg ttgtttttct
    21361 tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg atggtcaatc
    21421 accatcagcg tttcagcgag ataaaaacag aaatcaggac agttattttc cgctgccagt
    21481 tgtggtaaat cttcaaatcc cgccacaagg tcataagaga acagaccgcc gaagaacatt
    21541 gcttctcgtt cttcattcgg tacattcaac agactctgca ataagcggaa agcgtcaaaa
    21601 accgaaaggg agcataagcg agcgtcttca tccagcagtg gactgacagg ggggaagcgc
    21661 agcacacggc agtttggtga ttgttcattt tccacacccg caggcagggc gttatccagt
    21721 agtgccagca gggcttcgcc attgccggaa agtgcctgga ttgtgacagt gtcacctaaa
    21781 gctgtaatgc gcagcgcact gtctaccagc agcaggcttt ttaaatcatc tttgctgtcg
    21841 atatctgcgg attccagtag cagcgttgct ggacgatcct cacacaactg gtgaaaaagc
    21901 gcggtgggat tgtcgcgata agcgccttcg caggttagca gttcgagagt cggtttttgt
    21961 gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag gcgggctggg
    22021 tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc gccaccaacc
    22081 tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt gcgtactagt
    22141 taactagttc gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat
    22201 gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattacg
    22261 cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag
    22321 cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc gaccatgaca
    22381 ccacagctgc aatctcacca gcaagagagg aaatttcacg ttcgggactg gcgctgaatc
    22441 ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat attgtcgggc
    22501 tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag acagaacggc
    22561 gcaatcagcg ggcgcagctg attgccgaaa gacttgaaaa agcgcaaata cctggggcac
    22621 tggaaggcgc gcaacgactg gcgcagggcg gtgcggtgac gcgcggtcat tttgctcgtt
    22681 tccttgtgga gtgcggcaaa gccagttcaa tggcggatgt ctttaaaaag tatctggcgc
    22741 gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct attgatgtca
    22801 ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat cttagtgcta
    22861 aatggctgaa aagattggtt gcgcattttg ccgaacacta cggcgacgcg atggaagtcg
    22921 cgcagtgcca gcaatcgccc aatgaacgca cccagctggc gacacttgcg cgtcagcatc
    22981 atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc gaactgggtc
    23041 gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa cagccgcaga
    23101 acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata acccgcagca
    23161 acgcctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg tttatccaac
    23221 tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgcgatgg agcgtatttg
    23281 tcgaattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg atctttctga
    23341 gctctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa acaacacgcc
    23401 gggcaactac accttcatcc tgaaagggac gaaggaggtg ccacgccgtc tgttgcagga
    23461 aaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg cgctgcttga
    23521 agcattgggc gaaccgatgc tttccacatc actgatgctg ccaggcagcg aatttaccga
    23581 atccgatccg gaagaaatta aagaccgtct ggaaaaacag gtggacttga ttattcacgg
    23641 cggctatctc gggcagaaac caacaacggt tattgatctt actgatgata cgccggtcgt
    23701 ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa caaattgcgt tccacggatg
    23761 gaagactatg gcagggatat atcgctgcat tttgctgttg atagttgggc tttttttctc
    23821 ttcgttgagt tatccgaaaa acacggagat ccctttttat gaagaaggga tctcgctctt
    23881 tgatgttgaa gccactctgc aaccggatgg ggtgctcgac atcaaagaaa atattcattt
    23941 tcaggcgcga aatcagcaga ttaagcacgg attttatcgt gatttaccac gactctggat
    24001 gcagcctgat ggggacgctg cactgctgaa ctatcatatt gttggcgtca tccgtgatgg
    24061 tattcctgaa ccctggcatc ttgactggca tatcgggtta atgagtattg tcgtgggcga
    24121 taaacaacgt ttcttgcctc aaggcgacta tcattatcaa attcattatc aggttaaaaa
    24181 tgctatcctg cgtgaggggg attctgatct gctaatctgg aacgtgaccg gtaaccactg
    24241 gccgtttgaa atttataaga ccctattttc actcaagttg cctgatattg cgggtaatcc
    24301 atttagcgaa atcgatctct ttactggaga agagggcgac acatatcgaa atggccgcat
    24361 ccttgaggac ggaagaattg aatctcgcga tccgttttat cgtgaagatt tcacggtcct
    24421 ctaccgctgg cctcacgcat tacttggtaa tgccccggct ccacaaacga cgaatatttt
    24481 cagtcatctt cttttaccct caacgtcatc gttgttaatt tgctttccga gtctgttcct
    24541 ggcgtgtgga tggttatatc tctggaagcg caggccgcaa tttacgccgg tagatgtgat
    24601 tgaaaccgat gtcattccgc cagattacac gcccggcatg ttacgtcttg atgcgaaact
    24661 ggtttacgac gataaaggtt tttgcgccga tatcgtaaat ctgattgtga aagaaaaaat
    24721 tcatctggaa gatcattatg acaagaacca gcaaatcctg attcgtgtta atgaaggcgc
    24781 gaccagaaat aatgcggtat tactgcccgc agagcagtta ttactggaag cgttatttcg
    24841 taaaggcgat aaggtcgttc ttacggggag acgcaacaga gtcttacgca aggcattttt
    24901 acggatgcag aaattttatc tgccgcgtaa aaagtcttcg ttttaccgac ctgatgcgtt
    24961 tttgcaatgg ggcggaatgg caatattggc ggtcattctc tacggtaacc tgagtcccgt
    25021 aggctgggaa ggaatgagtc tggtgggcga tatgtttatt atgatctgct ggcttcttcc
    25081 ttttttattt tgttcccttg agcttttgtt tgcccgcgat gatgacaagc cttgcgttaa
    25141 tcgtgtcatc atcactttgt ttttaccact gatttgttca ggtgtggctt tttactctct
    25201 ctacatcaat gtcggagatg tattctttta ctggtatatg ccagcgggtt attttagcgc
    25261 tgttttcctg accggttatc tcactggcat ggggtatatt tttctgccaa agtttaccca
    25321 aactgggcag caacgttatg cccacggtga agctatcgtt aactatcttg cgcgtaaaga
    25381 ggcagcaaca cacagtgggc ggcgtaggaa aggggaaaca cggaaactgg attacgcttt
    25441 gttaggttgg gctatatcgg caaatttggg gagggaatgg gcgttacgca tcgccccttc
    25501 gctttcttcg gcgatttgcg ctccagagat tgcccgtaac ggcgttttat tctcattaca
    25561 gacgcacctg agttgcgggg cctataccag tttgttgggg cggagctatt ctggtggtgg
    25621 ttctggcgct ggtggcggag gcggtggtgg ctggtaatca agctgatgtt aatcgaaaca
    25681 agctttaacc ataaagatag tgtagtatgt tgcgcctcaa agccaggcaa gttaaaacgt
    25741 cgagtcgttt acttaaggcc tgaagagttc aaacagactt tactatcagt gagtcagatg
    25801 taaattctga ctcactgttg cacaaaagca tatcagacgc ctgggaaggc gacaccaaaa
    25861 ggaagctcta tgagcgaaaa gctacagaaa gtgctggcgc gtgccggcca cggttctcgc
    25921 cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa
    25981 ctcggcgatc gcgttgaaat tacccctgga ttgaaaatcc gtatcgatgg tcacctgatt
    26041 tcggtacgtg agtccgctga acaaatttgt cgcgttctgg cctattacaa accggaaggt
    26101 gagttgtgta cccgtaacga tccggaaggg cgtccaacgg tgtttgaccg tctgccaaag
    26161 ctgcgtggcg cacgctggat tgccgtaggt cgcctggacg ttaatacctg tggtctgctg
    26221 ctgttcacta ccgacggtga actggcaaac cgtttaatgc atccaagccg tgaagttgaa
    26281 cgtgaatacg ccgtgcgcgt atttggtcag gttgacgacg cgaaactgcg tgatttgagt
    26341 cgtggcgtgc agctggaaga tggtccggca gcctttaaaa ccatcaagtt cagcggcggc
    26401 gaagggatca accagtggta caacgttact ctgaccgaag gccgtaaccg tgaagttcgt
    26461 cgtctgtggg aagcggttgg tgtgcaggta agccgcctga tccgtgttcg ttacggtgat
    26521 atcccactgc cgaaaggtct gccacgcggt ggctggacgg agctggacct cgcccagact
    26581 aactatctgc gcgaactggt ggagctaccg ccagaaacca gctctaaagt cgctgtagaa
    26641 aaagaccgtc gtcgtatgaa ggcgaatcag attcgtcgtg cggtgaaacg tcactgtcag
    26701 gtgagcggcg gtcgtcgttc tggtggacgt aataataac
//
LOCUS       DAZGAP010000060        26552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGAP010000060 DAZGAP010000000
VERSION     DAZGAP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.060"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1380)
                     /gene="ycjM"
                     /locus_tag="ACHLAM_002086"
     CDS             complement(<1..1380)
                     /gene="ycjM"
                     /locus_tag="ACHLAM_002086"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO7853612.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSTFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYIRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     IDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELN"
     gene            complement(1594..1908)
                     /gene="pspE"
                     /locus_tag="ACHLAM_002087"
     CDS             complement(1594..1908)
                     /gene="pspE"
                     /locus_tag="ACHLAM_002087"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO7853613.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1983..2204)
                     /gene="pspD"
                     /locus_tag="ACHLAM_002088"
     CDS             complement(1983..2204)
                     /gene="pspD"
                     /locus_tag="ACHLAM_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO7853614.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2213..2572)
                     /gene="pspC"
                     /locus_tag="ACHLAM_002089"
     CDS             complement(2213..2572)
                     /gene="pspC"
                     /locus_tag="ACHLAM_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO7853615.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2572..2796)
                     /gene="pspB"
                     /locus_tag="ACHLAM_002090"
     CDS             complement(2572..2796)
                     /gene="pspB"
                     /locus_tag="ACHLAM_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO7853616.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2850..3518)
                     /gene="pspA"
                     /locus_tag="ACHLAM_002091"
     CDS             complement(2850..3518)
                     /gene="pspA"
                     /locus_tag="ACHLAM_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO7853617.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3685..4662
                     /gene="pspF"
                     /locus_tag="ACHLAM_002092"
     CDS             3685..4662
                     /gene="pspF"
                     /locus_tag="ACHLAM_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO7853618.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4782..6047)
                     /gene="puuE"
                     /locus_tag="ACHLAM_002093"
     CDS             complement(4782..6047)
                     /gene="puuE"
                     /locus_tag="ACHLAM_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO7853619.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENVTLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKMFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDEAMKILQDALSD"
     gene            complement(6085..7365)
                     /gene="puuB"
                     /locus_tag="ACHLAM_002094"
     CDS             complement(6085..7365)
                     /gene="puuB"
                     /locus_tag="ACHLAM_002094"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO7853620.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7367..8854)
                     /gene="puuC"
                     /locus_tag="ACHLAM_002095"
     CDS             complement(7367..8854)
                     /gene="puuC"
                     /locus_tag="ACHLAM_002095"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO7853621.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9004..9561)
                     /gene="puuR"
                     /locus_tag="ACHLAM_002096"
     CDS             complement(9004..9561)
                     /gene="puuR"
                     /locus_tag="ACHLAM_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO7853622.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9588..10352)
                     /gene="puuD"
                     /locus_tag="ACHLAM_002097"
     CDS             complement(9588..10352)
                     /gene="puuD"
                     /locus_tag="ACHLAM_002097"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO7853623.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10564..11982
                     /gene="puuA"
                     /locus_tag="ACHLAM_002098"
     CDS             10564..11982
                     /gene="puuA"
                     /locus_tag="ACHLAM_002098"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO7853624.1"
                     /translation="METNIVEVENFVQQSEERRGSSFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12131..12295
                     /locus_tag="ACHLAM_002099"
     CDS             12131..12295
                     /locus_tag="ACHLAM_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853625.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12285..13670
                     /gene="puuP"
                     /locus_tag="ACHLAM_002100"
     CDS             12285..13670
                     /gene="puuP"
                     /locus_tag="ACHLAM_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO7853626.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            13804..14049
                     /gene="ymjA"
                     /locus_tag="ACHLAM_002101"
     CDS             13804..14049
                     /gene="ymjA"
                     /locus_tag="ACHLAM_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO7853627.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14362..16005
                     /gene="sapA"
                     /locus_tag="ACHLAM_002102"
     CDS             14362..16005
                     /gene="sapA"
                     /locus_tag="ACHLAM_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO7853628.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16002..16967
                     /gene="sapB"
                     /locus_tag="ACHLAM_002103"
     CDS             16002..16967
                     /gene="sapB"
                     /locus_tag="ACHLAM_002103"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO7853629.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16954..17844
                     /gene="sapC"
                     /locus_tag="ACHLAM_002104"
     CDS             16954..17844
                     /gene="sapC"
                     /locus_tag="ACHLAM_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO7853630.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17844..18836
                     /gene="sapD"
                     /locus_tag="ACHLAM_002105"
     CDS             17844..18836
                     /gene="sapD"
                     /locus_tag="ACHLAM_002105"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO7853631.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18838..19644
                     /gene="sapF"
                     /locus_tag="ACHLAM_002106"
     CDS             18838..19644
                     /gene="sapF"
                     /locus_tag="ACHLAM_002106"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO7853632.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(19693..20856)
                     /locus_tag="ACHLAM_002107"
     CDS             complement(19693..20856)
                     /locus_tag="ACHLAM_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO7853633.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(20865..22238)
                     /locus_tag="ACHLAM_002108"
     CDS             complement(20865..22238)
                     /locus_tag="ACHLAM_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO7853634.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAAQETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQIGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(22242..25349)
                     /locus_tag="ACHLAM_002109"
     CDS             complement(22242..25349)
                     /locus_tag="ACHLAM_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO7853635.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(25349..26470)
                     /locus_tag="ACHLAM_002110"
     CDS             complement(25349..26470)
                     /locus_tag="ACHLAM_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO7853636.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
BASE COUNT         6162 a         7104 c         6765 g         6521 t
ORIGIN      
        1 gttcagttct gtcgttatct ctttactaga gtatttttta cggttaatcg cacgattata
       61 tccaagtttt tcgacacctg cgtaatcatt acgcgacccc agaatgcttt gaatatatat
      121 cgctggcaca ccggggaaac ttaacaaaat cgcatgggcg agaataaacc tggcacaccg
      181 ttcttcgtcg ctacactcac ggcggctcaa cgcatccata taggtgacat tcatttcata
      241 ggggctacgc gtaccgtcgg gattattttt ccagtttact aatgcccctt cctgctgtaa
      301 cacctcgacc agcgctaata tttcgttttc gggcaacaac ccccgcagtg ggttaagccc
      361 gatgccatcg tgagaggcaa ggaagttaaa ccaggtggtg ttgctggaag gtagtgtcag
      421 gttttgcgcc cacgcacaaa gtgcctcaac gttctgtttt tgcaccgcat gcagcaccag
      481 cggcggcagc gagaactggt acaccatatg cgcttcgtca tcaccgttgc caaagtaagc
      541 aatgttgtct atatgcggaa cattggtctc ggtaatgatc actgtgcctg gcgcaacgtt
      601 atcaataatc gaccgtaaca gtttgataat cagatgtgtt ttttccagat ggatgcagct
      661 tgttcccggc tctttccaca taaagccaac ggcatccaga cggatatact cagcaccttt
      721 ctcaaggtaa cacagtaaaa catccaccat cgccagtaac acttcagggc tacggtaatt
      781 gaggtcaatt tgatcgtcgc taaaggtggt ccataaatga cgcgttgaat tatcgcgcat
      841 ctggaatggc gttaataacg gtaacgcacg cggacgggtg acggcgctga gatcggtttg
      901 cggatcaacc gcaataaaaa aatcttcaaa acctggctgc tgttgtaaat agtttttaaa
      961 ccattcactt tttgccgaca tatggttgca gacaaaatca aacattaaat gactgcattc
     1021 accgagttgc tgaatatcct gccactcccc cgcttcactg gcgacctgat gataatcaat
     1081 taccgaaaag ccatcatcag atgaccatgg ataaaacggc aacaaatgaa catgtgaaaa
     1141 agtgctttgc agccattgat ggtaaaactg attaaatgtg ggtagtggtt ttaaatcatt
     1201 actgtgaaat tgatcggcat aggtaattaa cacgacatca ctttcatccc agtgtttttt
     1261 acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc gttacaagtt tctgtaaatg
     1321 agtcgcggta aatgttccac cgtagatttc gtccaggtaa tccgtaattt tctgttccat
     1381 gttgcctttt cgtggtagcg gtcccatgaa acgagagact actccgcaga aaataaactg
     1441 tcaattgagc acaagggttt tagtcacgca ggaaatcatt ttttaccagg gaaaaagcgt
     1501 atttgtgagc tgacgcaagt aatctatggg gatgtaaata aggtgtgaca gaaaaagtaa
     1561 acggcgcata agcgccgctt agggtggatt cttttaacct ttgaccttcg gcattgcgat
     1621 gtctttcagg ccgccggcgt tctcaacgtg ggtatatccc atctcgctaa ggatctcttt
     1681 tgcctgccct gactggcgtc cggcattgca atacactttc acggtgtcgt ttttatccgg
     1741 aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg ctccctgaac
     1801 gtgctcttgc tgatactgct ctggaacacg aacatcgatc cagtgttcag cggcgaaaac
     1861 gggcagtgaa aacaccagtg tcagagcaag taagcctttt ttaaacatgt aagcgtcctt
     1921 ctatggatga aaattgccat ccataatacg ccttagcttt aacaattttt gcgaaactcc
     1981 cctcaccttt tataacgctg cgccagttta ttggcagccc gacttaacag cggttccagc
     2041 gccacagcca gcaacatttt cagtggtcgg cgagcaactg attttatcgc ccagccagct
     2101 acacccgccg ggccatagcg cagtgcggta agaagtacca gcttgcctgc taatttgaaa
     2161 ccaggcttca ccttttgccc ggcctgttgc cagcgagtat tcataacttt cctcacagtt
     2221 gacggaaacg gctacgtaac gtgaaagtat cggaagtgac ataacgttcc atctcgcgta
     2281 aacgcgtttc acttgccgcc agttcacggt cgacttcatc cagcaattca ctgctggaag
     2341 gtagctgctc accaaaggcc atgttgtccg gcattggatc aagcgcaaat gacaaaatga
     2401 tgtaagcaac cagggtaaac agcgccagac cgaagaaaat cgacagcacc accaggatac
     2461 gcaccagttt tactggtaca tcaaaatagt tggcaatccc ggcgcagacg ccacggacca
     2521 tgccctgctg tggaatacgc cataattttt tattgagatt aatgcccgcc attagcgatc
     2581 cctccagttc ggatgttctg catcaagaat ggattccagc gcctgaatac gttcgcgcat
     2641 ccgttttgct tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg acaattcact
     2701 gcgaccagaa cgattgctgt aatgcagcca taaccagatc ggtaaaacaa acagcacaaa
     2761 aatggttaac ggaatagcca gaaatagcgc gctcataagg actccttaga cgatgagcgg
     2821 cggcgcgttc ggacgccgcc tggatgttat tattgattgt cttgcttcat tttggctttt
     2881 aattgtgcca gttgctcgct gattgcatca tcggctttca gttcggcaaa ctgatcgtcc
     2941 agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg gtcaattcga
     3001 cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccactgtc cagctgacga
     3061 cgcacatcgc gcgacgagtt tgccgcctga tggcgtaaca tcaatgcctg ctggcgagcg
     3121 cgtgtttcgc tcaatttgtt ttccagctcg ccgatctctt tcttcatgcg tgccagcgta
     3181 tcgtccacca gcgtcacttc atgttccagg ctcttaatca gatcggtcag tttctgtttt
     3241 tcaattaacg ccgcacgcgc caggtcttct ctctctttca gcagcgccag ttcggctttt
     3301 tcctgccatt caacttcacg cgccgacgct tgttcaatac ggcgagtcag ctgtttcttt
     3361 tctgccagcg cacgcgccga agtagaacgt acttcaacca gtgtatcttc catctcctgg
     3421 atcatcagac gcaccagttt ctgtggatct tccgctttct ctaacagagc gttgatgttg
     3481 gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc ttgatttctg
     3541 cgttcaggat tgtcctgctg aactattaat acaatttgcg tgccaatttt ttatcttttt
     3601 gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgagt tagcgaatta
     3661 cactaacaag tggcgaattt catcatggca gaatacaaag ataatttact cggtgaggcg
     3721 aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga taaaccggtg
     3781 ctcatcatcg gcgagcgcgg cacaggtaaa gagctgattg ccagccgtct gcattatctc
     3841 tcctcccgtt ggcaggggcc gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg
     3901 ctggattccg aactgtttgg tcacgaagcg ggggcgttta ccggtgcgca aaaacgtcat
     3961 ccagggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact cgctacggca
     4021 ccgatgatgg tgcaggagaa attattgcgc gtgattgagt acggtgaact ggagcgcgtt
     4081 ggcggcagcc agccattgca ggtgaatgta cggttggtat gcgcgacgaa tgccgatctc
     4141 ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc tcgaccggct ggctttcgat
     4201 gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca ttatgctgat ggcagaacac
     4261 tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt tcccgggttt tacggagcgc
     4321 gccagagaaa cattgctgaa ttatcgctgg ccgggaaata ttcgtgaatt gaaaaacgtg
     4381 gtggaacgtt cggtgtatcg ccacggcacc agcgattatc cgcttgatga catcattatt
     4441 gatcccttta aacggcgtcc gcctgaagac gctatcgccg tttcagaaac cacctcgctt
     4501 ccaacgctgc cgctggattt acgtgagttt cagatgcagc aggaaaaaga gttgctgcaa
     4561 ctcagtttgc aacaagggaa atataaccag aagcgcgcgg ctgaattact ggggttaacc
     4621 tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg ggcaatacaa
     4681 cttgccggat gcggcgtgaa cgccttatcc ggcatacata atcaatacgt tgtttgcagg
     4741 cctgataagc gcagcgcatc aaacattatt gcgttgggct attaatcgct cagcgcatcc
     4801 tgcaaaattt tcattgcctc atcgaattgc gcatccggga tggtcagcgg atacaggaag
     4861 cgaatcacgt tgccgtatgc gccacaggtc agcaagagca gcccctgcgc cagcgcgcgt
     4921 tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc gttaaactct
     4981 gccgcaatca tagaccccag accgcgtacc gccgcgatgg ccggaacgct ttctttggca
     5041 tcaatcaacg tgtttgtcag gcgctggccc agttgattcg cgcgttcaca gagtgattct
     5101 ttgtcgataa tgttgagcac agcgtgcgcg gcagccaccg ccagcgggtt cccggcgtag
     5161 gtaccaccca acccgcccgg cgcgggcgcg tccataatat tcgcgttacc gaccacgccc
     5221 gaaagcggca tcccgcctgc gaggcttttc gccatcgtca ttaagtccgg cttatcggcg
     5281 tagtgatcca tggcaaacat tttaccggta cgcgcaaagc cgctttgcac ttcatcggca
     5341 atcatcacaa tgccgtgctc gtcgcacagg cggcgaatgg cggcaaccag ctcttttggc
     5401 gcaacgttga aaccgccttc gccctgaacc ggttcgaaaa taatcgccgc cacctgcttc
     5461 gcttcaatgt ccgatttaaa caagcgttcg atggcgtcga gggagtcctg cgttgaaatg
     5521 ccgtgtaaat ctgacggata aggtacgtga tataccgaac cggggaacgg gccgaagccg
     5581 attttgtacg gcgcgacctt tccggtcagc gccatggtca tatatgtacg accgtggaag
     5641 ccgccgctaa acgcaatcac gccagggcgt ccggtatggg cgcgggcgat tttcaccgcg
     5701 ttctccaccg cttccgcacc ggtggtgaag aacgcagttt tagcctgccc gctcaccggg
     5761 gcaagggcgt taattttctc cgccagcgtg acgtagcttt cgtacggcac aatctgatac
     5821 gccgtgtggg taaactgttg caattgctgt tccaccgccg cgaccagatc agggtggcga
     5881 tgcccggtat tcagtaccgc aatgcctgcg gcgaaatcga tatattcgtt gccctctaca
     5941 tccttcagcg tgacgttttc tgccgactgg gcgaagaagt tacacatcac gccaaccccg
     6001 cgtggggtag cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt
     6061 tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaataagc
     6121 cgcgcccatc gcggtaaacg gaacgcgcag cgtgcgcccg ccggggaacg ggtaatgcgg
     6181 cagattggcg aaggcgtcga aacgctcggc gtcaccgcgc agcagttcag caatcaaacg
     6241 cccggcgagg tgggtacagg tgacgccgtg gccgctgtag ccctgcatgt agtagatgtt
     6301 ggtatcgagg cgaccaaact gcggcatgcg tgacagggtc agcaggaagt tgcccgtcca
     6361 gcggtaatca attttcacgc ccttcagttg cgggaaggtt ttcagcagtt tcggcaccac
     6421 aaggcgctca acatcgtccg ggtcgcgtgc gccgtagacc acgccgccgc cgtacagcag
     6481 gcggttatcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt ccacacagta
     6541 gtttttcggg atcagcgaac gggctaaatc ttccgacagc cgttcggtgg tgatcacctg
     6601 cgtgccgcac ggcatgctgc gtttcgctag ttccggctct actttatcgc ccaggtaggc
     6661 attcccggcg acaatcacat acttcgccgt cacctgacca ttggcagtcc gcaccacggc
     6721 tggcgtggtg tgctggatct gcgtcactgc ggaaagctca tgcacgcgcc cgccgttcag
     6781 gcggatggca tcggcttcac caatggcgag gtttagcggg tgaatatgcc caccgctgtg
     6841 atccagcagc gcaccagtgt agcgatcgct ggcgacctcg cggcgaatag cgttggcgtc
     6901 cagcagctcc agctgtttat tgccgtagcg ttcccagttc tctttctgct cttcgagtgt
     6961 ggcgagctgt ttatcattca tcgccacaaa cagaccgccg gggcgatagt cgcagtcaat
     7021 ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca tgctgccgag
     7081 catacgggcg gtatccatgc cgtagctttt ttcgatcacg tcgatgtcgc ggctgtagga
     7141 gttcacaagc tgcccgccgt tgcgtccgct cgcgccaaag ccgatgcgtg aggcttcgag
     7201 aaccaccacg tcaaagcccg cttccgccag atgcagcgcg gaggaaagcc cggtatagcc
     7261 gccgccgacc acgcaaacgt cgcaggaaat cgactcattc agcgtgtcga atggcgcgta
     7321 tttattcgca ctggcggcgt agtaactgct ggtgtgttcg gtcatttcag gcctccaggc
     7381 ttatccagat ggttttcagt tcggtgaatt tttcaagggc atgcagggat ttgtcgcgcc
     7441 cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat
     7501 tgacgaagac ggagccggct ttcaggcggc gactcatgcg gtgcgcgcgg gagaggtcgc
     7561 gcgtccatac cgccgcgcca agtccgtact ggctgtcgtt ggcaagctgt agcgcctgtt
     7621 cttctgatgt gaaacgcgtg accaccagca ccggaccgaa aatctcttcg cggcttaagg
     7681 acgcattcgg gtctacctcc acaaagatgg tcgggccgat ggcggcagcc agctcggcgt
     7741 tacggccatc cagcagcagt tgccctttgc tttcgccttc ccgaataaag ctatggaccg
     7801 agtcggcgtg ggcgcagtcg attaaggtgc ccatggtggt tgcgggatca agcggatggc
     7861 ccggctgcca gttttgcgct tgctgtttta acagggctaa gaattcatcg gcgatgctct
     7921 cttccagcaa caggcgcgtt ccggcgatac acacctgccc ctggttgtag aaaatgcccg
     7981 cagcggtcgc gctcgccgct ttttgcaaat ccggacagtc ggcgaaaacg atgttggcgc
     8041 ttttgccgcc cgcttccagc cagacgcgtt tcatgttgct gtcgcccgcg tccttcagca
     8101 gctgtttccc ggtgcgggtt gagccagtaa aggcaatggc atcgatatcg ttatggcgcg
     8161 aaagcgcctg cccggcttca tgaccaaaac ccgtcaccac gttcaacaca ccatccggca
     8221 gccccgcttc tttcgccagc cccgcgagac gaatcgcact gagcggtgat ttttcagacg
     8281 gttttagaat cacgctgttt cccgccgcca gcgccgggcc gagtttccag caagtcagca
     8341 gcagcgggaa gttccacggc acgatggcgg caatcacgcc gaccggttca cgcacgatca
     8401 tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg
     8461 cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc acgcaggctg tgacgaatcg
     8521 gtttaccggt gtcgagcgtt tccagcagcg ccagttcttc agcgttggct tccattaaat
     8581 cggcgagttt attcagcacc gctttacgct tagccggaga agagagtgac cagtcgccac
     8641 gttcaaacac gccgcgtgct gcgctcaccg cacggtcaat atcgacgctc ttgccgcggg
     8701 caatattcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaagtt tcattttccg
     8761 ccgcagcagt atattcaccg ttaataaata agcgggtttc aatggcgaga cttaacgctt
     8821 tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag
     8881 atatatgcag tttgtgaacg tcagggcgca gagatccccg ggccgtatgc ccgtttattt
     8941 ctggcgacat tctttataga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
     9001 agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt
     9061 actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga cgagatgata
     9121 atcctgacca ttaatcgtca gaacaatttc accttccagt acagtgccta tttcctcacc
     9181 ctgatgctta attctttccc cagtggttgt gcccggctgg taagtttcaa agatcatcgc
     9241 cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat
     9301 ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
     9361 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
     9421 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
     9481 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
     9541 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
     9601 ttttcagcga tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc
     9661 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
     9721 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
     9781 ctaacgacct tcgccccttg tccatgcaga gagtttaccc aaaagttgct acattcaggt
     9841 aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg cgcatattgc
     9901 tgctccaccg gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc
     9961 ttgcgatgca acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg
    10021 aaaatgggga tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc
    10081 ccaggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
    10141 ggactaccag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taatgacggt
    10201 tccgccagcg catgtggcag cgcaattggc aagccgcctg cgtggatgat ggcattcagg
    10261 tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca
    10321 ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta
    10381 tcgccattac tgcgctttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg
    10441 ttttcatttt tgcaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg
    10501 aatagtggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
    10561 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
    10621 ggtagctcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat
    10681 gttgatgttt tacttaccga tttgaatggt tgcttccgtg gaaaacgtat tcctgtttca
    10741 agcctgaaga agctcgagaa agggtgttat ttcccggcct cggtgtttgc aatggatatt
    10801 ctgggcaacg tagtagaaga agcgggcctg ggtcaggaaa tgggcgagcc ggatcgtacc
    10861 tgtgttcctg ttctcggttc cttaacccct tctgccgccg atccagagtt tatcggtcag
    10921 atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt
    10981 cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggtcgt agcggtagag
    11041 ctggagttct atttactgga tcgccagcgc gacgctgaag gatatctgca accgccctgc
    11101 gcgcccggca ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac
    11161 cacttcgccg acgtgctcaa tgatattgat gagctggcgc agttacagct gattccggca
    11221 gatggcgcgg tcgccgaggc ctcgccaggt cagtttgaaa tcaatcttta ccataccgat
    11281 aacgtgctgg aagcttgcga tgatgcgctg gcactaaaac gcctggtgcg tctgatggca
    11341 gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc
    11401 gggatgcata tccatatcag tatgcaaaat aatcgtggcg agaacgtgct ttctgacgcg
    11461 gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg
    11521 tcatcaatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccggggatg
    11581 tatgtaccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccc
    11641 tgcggcgatc gccataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaacccg
    11701 tatctggtga tggcggcgat ttttgccggt attttgcatg gccttgataa cgaactgcca
    11761 ttacaggaag aagtcgaagg taacgggctg gaacaggaag gcttaccctt cccgattcgc
    11821 cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa
    11881 cgcttctgcc atgtctatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc
    11941 atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aatacccctt gcggggttgt
    12001 ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat
    12061 gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg
    12121 aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc
    12181 tcctgcgact gactggactc cgccccctct gcttcccttg ctaaacaggt ttgatttcag
    12241 gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac
    12301 cactgaatat tgctgcgcaa cctggcaaaa cccgtctgcg aaaatcactg aaattgtggc
    12361 aggtggtgat gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta
    12421 ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg
    12481 tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg
    12541 cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc ggctggtcat
    12601 cgctgctgga ttatctcttt ttgccgatga tcaacgtttt gttggcgaaa atctatctct
    12661 ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtt gccattttaa
    12721 ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccttg tttgtactgg
    12781 tgcaaatctc catcatggtg gtgtttatct tcctggttgt tcagggactg cataaaggag
    12841 aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc
    12901 caattattac cggggcgacg attgtctgtt tctccttcct cggtttcgat gcggtgacca
    12961 cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg
    13021 cggtatacgg cggcgttatc tttatcgctg cgtcgttctt tatgcagctg ttctttcccg
    13081 atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgcta tacgtcggcg
    13141 gcaagctctt ccagtcgatt ttcctctgta ccacgtttgt gaacacgtta gcgtctggcc
    13201 tggcgtcgca cgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc
    13261 cggagcgcgt gtttggctat gtgcacccga aatggcggac tccggcactg aacgtcatta
    13321 tggtcgggat tgtcgcgttg tcagcgctgt tctttgattt agtcaccgcg acagcattga
    13381 ttaacttcgg tgctctggtg gcatttacct tcgtaaatct gtcggtgttt aatcatttct
    13441 ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc
    13501 tggttggcgc gctgacggtg ggtgtgttgt gggttaacct cgagtcaacg tcactgacac
    13561 tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc
    13621 gcaaagtgcc gttgtatgat ggtgacagaa cgccggtgag cgaaacgtaa tagtcggatg
    13681 cgcggcgcat tatcctgcct gagggtaatg caccgcgcca ttaactataa aaagatgtat
    13741 gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa
    13801 ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa
    13861 tgcgcagagc ctgtcgcaga cgctgaacgc acaccgctgg ccctctactt ccagttactg
    13921 ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct
    13981 gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt
    14041 aatgagtaat tatttcattg cagactctcg gtcagtatcg ttgtccggat aaagcgttca
    14101 cgccgcatcc ggctaacaat gacacgcact gcagcgtgcc tcaatgggtt tctcacgatc
    14161 gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt
    14221 acgaatgttg aacagatggc gacgtcatct gttcaacgac caaccacacg gccctcatta
    14281 cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt
    14341 tacataaaat ccattaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga
    14401 cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac
    14461 agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc
    14521 agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat
    14581 ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc
    14641 gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact
    14701 cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc
    14761 aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt
    14821 gccgataacg tgaaaagcgt ccgtaaactg gataatcata ccgttgagtt ccgtctggct
    14881 cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca
    14941 gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcagccggtc
    15001 ggcactggac cgtatcagtt gtcggaatac cgcgccgggc aatatattcg cttacaacgt
    15061 catgatgatt tctggcgtgg taagccgttg atgccacaag tggtggtgga tttaggctct
    15121 ggcggtacag gacgattgtc gaaactcctg accggggagt gcgacgttct ggcctggcct
    15181 gctgccagtc agctatccat tttgcgtgac gacccacgct tgcgtttaac gctgcgcccg
    15241 gggatgaacg tcgcctatct ggcgtttaac accgctaaac cgccgctaaa taaccccgct
    15301 gtccgccatg cgctggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat
    15361 ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag
    15421 gctaaaatta ctgaatacaa tccggcaaaa tcgcgcgaac agttgaaggc gttggggctg
    15481 gaaaacttaa cgctgaaact gtgggtgcca acacgttcgc aggcgtggaa ccccagtccg
    15541 ctgaaaaccg ccgaactgat tcaggcggat atggcacagg ttggcgtaaa agtggtgatt
    15601 gtgccggtag aaggtcgttt tcaggaggcg cggttgatgg atatgagcca tgatctgacg
    15661 ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc gttactgagc
    15721 tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc
    15781 gtgttgcgta aggcgctctc ctcgcagcag ctggcggcgc gtattgaagc ctatgacgaa
    15841 gcgcagagta ttctggcgca ggaattaccc attctgccgc tggcgtcgtc attgcgcttg
    15901 caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt
    15961 gctggggtgt atcgcgagaa acaggatgag gtgaaaaagc catgattatc ttcaccttgc
    16021 gccgtatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga
    16081 gctatttcac cccacatgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct
    16141 ggtttaacgg cctgatccac tgggattttg gtgtttccag cattaacggt caaccgattg
    16201 ccgaacagtt aaaagaggtc ttcccggcga cgatggaact gtgcatcctc gccttcggtt
    16261 ttgcgctgat tgttggcatc cccgtaggaa tgattgccgg gattacgcgc cataagtggc
    16321 aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg
    16381 cgcttctgct gacgctgttt tgttcactca cactaggttg gttgcccgtt tcagggcgtt
    16441 tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt
    16501 cggactcacc atggcgggat gaaatggtca tgagcgcaat ccgccatatg atattgcccg
    16561 tgatcaccct gtcggttgcg ccaacaactg aagtgatccg actgatgcgt attagcacta
    16621 tcgaagtgta tgaccagaac tatgttaaag cagcggcgac ccgcggtttg tcgcgcttta
    16681 ctattttgcg tcgccacgtt ctgcataacg cgttacctcc ggttattcct cgcctgggct
    16741 tacagttttc taccatgctg acgctagcga tgatcaccga aatggttttt agctggccgg
    16801 gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg
    16861 gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg
    16921 ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa
    16981 aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg
    17041 atggtcggcc tgtacggctg cgcggggctg gctgtactgt gtatttttgg cggctggttt
    17101 gcgccttacg gcatcgacca gcaatttctc ggttaccaat tattgccgcc gtcatggtcg
    17161 cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgcga tgttttaagc
    17221 cgtttactga gcggagctgc gccaaccgta ggtggcgcat ttgtggtgac gcttgccgcg
    17281 acgatctgtg gcctggttct gggcaccttc gccggggcga cccacggcct acgctcagcg
    17341 gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgct ggcaattatc
    17401 gttgtagcgt ttgccggacc gagtttgtct cacgccatgt ttgctgtctg gctggcattg
    17461 ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac
    17521 gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg
    17581 cccaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc
    17641 gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga
    17701 gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca
    17761 ggtgcggcaa ttatgattag cgtgctgttg gttaacctcc ttggcgacgg tgttcgtcgt
    17821 gcaattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat
    17881 ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgcgt aagtatgacc ttaaccgaag
    17941 gtgaaatccg cggtcttgtt ggcgaatccg gttccggcaa aagtttgatt gcgaaagcga
    18001 tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata
    18061 tcgatttgct gcgtctctcc gcacgcgaac ggcgcaagct ggttggtcac aacgtgtcga
    18121 tgattttcca ggaaccgcag tcttgtcttg acccttcaga acgtgtgggt cgccagttga
    18181 tgcaaaacat cccagcctgg acgtataaag gccgttggtg gcagcgtttt ggctggcgca
    18241 aacgccgtgc cattgaacta ctgcaccgtg tggggattaa agatcacaaa gatgcgatgc
    18301 gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac
    18361 tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg gagccaacaa
    18421 cccaggcgca aatctttcgc ctgctgacgc gtctcaacca gaacagcaat accactattt
    18481 tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt
    18541 actgcggtca aacggtggaa accgcgccaa gtaaggagtt ggtgacaatg ccacatcatc
    18601 cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa
    18661 gtcgcctcaa tacgctgccc ggcgctatcc cactgctgga acagttaccg attgggtgtc
    18721 gtctggggcc gcgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg
    18781 gggcgaaaaa tcatctctat gcctgtcact tcccgctgaa catggagaaa gagtgagatg
    18841 atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgttaccg gaccggctgg
    18901 tttcgtcgtc agaccgtaga agcggtaaaa cctttgagct ttacgctacg tgaaggccag
    18961 acactggcga ttattggtga gaatgggtcg ggtaaatcca cactggcaaa aatgctggcg
    19021 ggaatgatag aaccgaccag cggcgagtta ttgattgacg atcatccgct gcattttggc
    19081 gattattcct tccgtagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg
    19141 aatccccgtc aacgtatctc gcagattctt gattttccac tgcgcttgaa caccgatctg
    19201 gaacctgaac agcgtcgcaa acagattatt gaaacgatgc ggatggttgg gctgctaccg
    19261 gatcacgtca gttactatcc gcatatgttg gcgcccggac aaaaacaacg tctgggtctg
    19321 gcacgcgcgc tgatattgcg tccaaaagtg attattgccg atgaagccct cgcctcactg
    19381 gatatgtcga tgcgttcgca gttgattaat ctgatgctgg aattacagga aaaacagggt
    19441 atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggta
    19501 ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgccgatgt gctggcatcg
    19561 ccgctgcatg aactcaccaa acggcttatt gccggtcatt ttggcgaggc attgacggcg
    19621 gatgcgtggc gtaaggaccg ttagtggttt gcacctcatc ctgacagggc ccccctcagg
    19681 atgaggtgtt cattactttt gattgtcgat tctggtgaga gcaaccagca atgccagcgt
    19741 ataacacacc gtaattgcca gactcatttt taacagtgtt tctccgccaa ttcccgacaa
    19801 tggcgcggct ataccgccga agacaaacat gctcatcccc atcaacgcag acgccgttcc
    19861 agattcctgc gtccctactg cactcatagc ctctgccccg ccaacagtgc cgatgccgct
    19921 acaaaatgcg atggtgaaaa ataatgccac cagtgccgga atgggcaatt gtatccatgc
    19981 gcataagacc gtgagcaatg cacacaaaat tgccgctatc aggccacctc gcaacaatgt
    20041 catcgcgtta atccgtcgcg ccaggcgcga gaagatccat gaagcaatga taagtccgat
    20101 gccgttaagg ccaaacacca ggctaaattg cattggacta aagccaaatt ccttctgcaa
    20161 cacgaacgac gaagagccga tatatgcaaa caaaccagcc agcataaaac tttgaatcag
    20221 gcaaaagcgt ataaaacggc ggttctgcac cacgcttcgt ccggttaata gcaatgatga
    20281 gcccctttta ttttctggca aggtctcatt aataaataac aggcagccca gcaacagtac
    20341 ggtgctaatt tcagccatta cccagaataa agtgcgccag tcaaaagtgc taacaatgta
    20401 cccgcccagc actggcgaca acaccggtgc caggccattc accgtcatta acagcgcaaa
    20461 aaactgggtc aacgttactc cctgatattt gtcacgagca atagaacgag agagcaccga
    20521 accacccgcc cccgcgatcc cttgaataaa acgccagacc accagccagt aaatattgtt
    20581 cgttgtcgcg cataaaatgg aagaaacaat aaacaatagc aacgacaaga ttaacggacg
    20641 tttacgccct attttgtcgc tcagagggcc aaataacagt tgtccgacgc caagaccaat
    20701 cagtgatgcg gtcagggtta attgcgttat ggtcgtggtt gccgccagtt gctcagacat
    20761 ctccggcaaa gccggcaaat aaagatcggt acacatcggg ccgataccgg ctaaaagacc
    20821 aagaatcaat gcccatgaaa gagagactct agccatatta ttccttactt gagtgatgac
    20881 ataccgccgc ccagcgactg ccagagcgtg attcggtttg taaaatcagt cagttgcagt
    20941 gccagcagct cctgctgtgc gctccacaag ctgcgctgcg ccgtcaacac ggtcaggtaa
    21001 tcaccaacac ccgcctgata gcgacgcaag ccaacatcga ccgtttgttg ctcagcttta
    21061 acatactggc gctgtgcatc cagttgctct tcaagcgtgg tccgccgcgc cagtgcgtta
    21121 gcgacatctt taaatgcgct ctgaacggtt ttttcatagg tggcaatgag cccacgcttt
    21181 tgtgcttctg cgtaacgaag ctgcgccaga ttacttccgc cagtgaacag cggtaatgtg
    21241 accgatggcg caaatgacca gatctgcatt ccgtggctga acaacgacga caatgcgtca
    21301 ctgccaacac cggcactggc agtcagcgaa atcgtcggga aaaagttagc ccgcgccgcg
    21361 ccaatatctg cattggcgct cttcaaatta tgttcagctt cctggatatc cgggcgacgc
    21421 agcagaacat cagatgatac tcccgcagga accagactta tcatttgttc cggcaggctt
    21481 tccagtgttc ccggtagcag attttccgca agcgttgtac ctgccagtaa gttcagcgca
    21541 tttttatcct gcatcacttg tgtctgatag ctggcaacgc tggcgcgcgc ttgctgataa
    21601 acactcatcg cctcactgac atctgtcgct gccgccgtgc caatctgttg ctggcgctgg
    21661 ataattttca atgagttttc tgcactggtc atcgtttctt ttgccagggc caggttgctg
    21721 ttatcagccg ccagtgtcag ccaggcggtg ctgatttcag caatcagcgt taaacgagtg
    21781 ttctgggctg taaattcact ggcaagccag gtttcctgcg ccgcccgaga taaactctga
    21841 ttacgaccaa acagatctaa cgtataactg gacaccgtgc cgtcagcctc ggcagttgtt
    21901 cccgtaccat tcgccagcga acgactgcga gtcgaactta aggccgcatt caccgtcgga
    21961 aataacgacg cattagtttg cccatagagc gcccgtgctg agtcaatatc agcaatcgct
    22021 ttttgcacat cgcggttact gttcagtgca atggttacta cctgctgcaa tcggggatcg
    22081 tggatcacct gctgccagtc atggctgatt gccttcccct ggccctgtgc tcccggcaag
    22141 gtcgccggga ttggggattc aggcgtactg tagtggggat cgagcgagac acatcccgca
    22201 ctcaacaaca ccagaaaaat aagagaacga cgcaacataa cttactcctg acggcgcggt
    22261 ttaccggcaa acaaacgttt caccagtaca aaaaacagag gaacaaaaaa aatagccagt
    22321 aatgtagcgg tcagcgtgcc gccaataatg cccgtaccaa tggcgatgcg gctgttcgcc
    22381 cctgcaccgg ttgctatcgc cagcggcatt acccccgcaa taaacgccag cgaggtcatg
    22441 atgattgggc gtaaacgagt ctgcgccgcg cgtaatgccg cacgactcaa ggaatagccc
    22501 tctgcaaccg ccgcttcagc aaattccaca atcagaatgg cgtttttcga cgacaagccg
    22561 atagtggtta atagcgccac ctggaagtaa acatcgttgt ttaaatcacg catccaggcc
    22621 gccagcgccg cgccaagaag ccccagaggg atcaccagaa tgaccgagaa cgggactgac
    22681 cagctttcgt acagtgctgc aaggcagagg aaaaccacca gaatagaaac tgcatacaga
    22741 ctcaatgcct gaccgctggc cagtttctcc tgcaatgaca aaccactcca ggcccaggtt
    22801 gttcccgcag gaagactgtt tgccagttcc tccatttttg tcattgcatc gccggaacta
    22861 aagccagtcg cattttcccc ctgaatttca taggctgcag aaccgttgta gcgcaccagt
    22921 ctttccggtc catacagcca gcgggtggtc gcaaaagcag agaatggtgt catggcgtta
    22981 tcgctaccgc gcacaaacca tttacctaag tctgacggcg cggaacgaaa ttcactgtcg
    23041 ccctgaatgt agactttttt cacacgacca cggtcaataa agtcattcac ataagtaccg
    23101 ccccacgcac tggacagggt gtcggtgaca tcattcaaac tcaaccctaa tgacaccgct
    23161 ttattactat caatatctac ctgcaattgc ggcatttgtg gcaaatcatt ggcacgcaca
    23221 gaatgtaact ccggactttg attcgctttt tctatcaact gattacgcat ttgcagtagt
    23281 gtttcgcgat cggttccacc gttagctaac aattcaaacg taaaaccatt gctttggccc
    23341 agcccatcaa cggctggcgg cgtcatcgca aataccgtgg catcacgaat tgtgcccagc
    23401 tctttggtag cccgtagggc gatagcctgg gcggtgtttt ctgccccttt acgttgagac
    23461 cagtttttca acgaaacaaa cgccatcccg gtgttctgtc cgctgccgct aaaactgaaa
    23521 ccatcaacgg taaaaatgac atcggtattt gctttctcgt taatcaggaa ccagtcaaca
    23581 atctggcgat tcacttctgc tgtacgggca gcggtcgccc cggccggcag cgtgtactgc
    23641 accatgattt cgccctggtc ttcagtgggt aagaaactgc ccggcagttt ccacatcatc
    23701 agggccattc caccacccag tacgacataa agccccatcg ttcgagcagc acggcgcagg
    23761 acataaatta cgcctcgctg atatttatct tcagtacggc ggtaaaagcg gttaaatgcg
    23821 ccgaaaaagc cttttttatg tggcggaaca tgctggagga cggaaccgca cagggcggga
    23881 gtcaaggtca atgccaccac cacggaaagc agcattgcgg agatgatggt gatggagaac
    23941 tgacgataaa ttacgcccgt agatccccca aagaaggcca tcggcaggaa tacggctgac
    24001 aacaccagcg caatggcaac cagtgcgcca gaaatctcgc ccattgattt ttccgtggct
    24061 tcacgcgcgg gcaacccttc gtcacgcata atgcgttcga cgttctctac caccacaatg
    24121 gcatcgtcca ccagcaagcc gatcgccagc accattgcaa acaacgtcag ggtattaatc
    24181 gaatagccaa acaacgcaag aacgccgaat gtgcccagca aaacgacggg gacagctaac
    24241 gccggaatca gtgtggcacg caggttttgc aggaataaat acatcacgca aaccaccaat
    24301 acgatggctt caaacagcgt ctgaattacg tcctctacgg agattttaat aaattcagtg
    24361 ctgtctttcg ggtatgcgat gtcgtatccc tgcggcatgt tccgctggaa ttcggcaatc
    24421 ttatccttga ccagcgtcgc cgtattcagc gcattcgcac cgggcgaaag cattacagca
    24481 accccggcag acgggtgacc gttaagtttg ccgatagcgg tataatcttc actgcccatc
    24541 tcaacgcgag ccacatcttt tatacgaaca actgcgccgt ctgactggct tttcacgata
    24601 atatttttga actgatccac cgtctgcaaa cgagactgcg cacgtaccgt tgcggtcagt
    24661 tgctgagtat tcggtgaagg caatgcccct attttcccgg cagtaacctg cacgttttgc
    24721 gcttcaatag cactttgcac gtctgaaggc attagcgagt aagacgccag tttggccggg
    24781 tcaagccaga tgcgcatagc gtattccgcg ccaaagactt gcagactccc cacaccttca
    24841 acacgcgcca gcgggtcctg aacgttacta accaaccagt cggcgatatc cgaactggag
    24901 gctttgtcag tggtgtcata cacggcagca atcagcaaaa agttgctttg tgatttctcc
    24961 accgtaacac ccgtttgctg cacttcgctg ggtaggcgcg actccgcctg ctgaatttta
    25021 ttctgcacct gaacctgtgc agtatctgga tcggtacctt gttcaaaggt cacattaata
    25081 ctgaccgaac catcagagct gctggttgag ctgaaataga gtaaattatc aagcccagtg
    25141 agttgttgct cgataacctg agtcacgctg ttctccagcg tttcggcaga agcaccagta
    25201 taagtggctg aaattttaat ggtcggtggc gcaacgtcag gatattgcgc gacgggcaat
    25261 gtgcgaatag ccagaatccc cgccaacata atcaaaatgg cgataaccca ggcaaagacc
    25321 gggcggcgaa caaaaaagcg cgaaaacatc aggcgttgcc tccattagcc tgaacttcaa
    25381 cagccttgac ggtctgccct gaagtgactt tggcagaacc ttcaacaatc agtcggtcgc
    25441 cgttgtgcag gccgttcagc accagccatt tatcaccata cgtttctccc gtttcgagcg
    25501 ttcgctgctc tactttattg tctttattca ccaccagcgc agttgcattg cctttagcat
    25561 cgcgcgtgac gccctgttgc ggcgcgagaa tagcgtcttc catcacgcct tcatcgacac
    25621 gagcgcgaac aaacattccc ggtaataact gctgttgtgg attgggaaaa atcgcccgta
    25681 atgtcaccga accggtagac tcatctacag cgacttcggt gagttccaga cgcccttttt
    25741 cgctgtaagt tgtgccatct tccagaataa gtgagacgct catggtgtca ctgttggtcg
    25801 ccagtgactg tttacgcaga cgtaataaat cgacactaga gcgagtgagg tcgacataca
    25861 ttgtatccag accacgaata gtcgttaacg ctgtatcctg cgacgcggtc accagtgcgc
    25921 caggggttac cgacgaaata ccaatacgcc ccgaaatcgg tgcggttacc gtggtccagt
    25981 caagattaat gcgcgcagtt tccagtgcgg cttttttcgc ctctacactg gctttatctt
    26041 gtgcacaggt agactgagca tcatcagcat cctgttgtga aacaccgttc tctttcacca
    26101 gtcgggcata acgctgcgct ttctggcaat cggcttttac cagtgcctgt gcttgttgta
    26161 atgctgcccg agcttcattc catgcagcct gataactggc cgcatcaatc tggtagagcg
    26221 gctgtccagc cttgaccaga tcaccttcct taaataagcg tttctggata attcccccaa
    26281 cctgcggacg tacttcagca ctgagcgcag cactggtgcg tccggttaat tcactgacta
    26341 ccgaaaccgg ttgagatttg agcgtaacaa cgccgacttc tgtttctgac ggggatgaat
    26401 tgttactttg cgtattatca caacccgata agagcagcat tgccactaca gatgttgcta
    26461 tatacttcat tattttctcc agggtgagaa ataccctcct cattgaaatt aatgaggata
    26521 tgtcagggat attcagttat aacgaacccg ca
//
LOCUS       DAZGAP010000061        26407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGAP010000061 DAZGAP010000000
VERSION     DAZGAP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.061"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1458
                     /gene="gspA"
                     /locus_tag="ACHLAM_002111"
     CDS             <1..1458
                     /gene="gspA"
                     /locus_tag="ACHLAM_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO7853637.1"
                     /translation="REVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKHRRVI
                     TLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIEN
                     AHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEHELKA
                     SILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFTAKHW
                     RMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPVVKQD
                     MTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTLVDQN
                     LPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLWKMSP
                     EGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLKTDGV
                     VGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1460..1879
                     /gene="gspB"
                     /locus_tag="ACHLAM_002112"
     CDS             1460..1879
                     /gene="gspB"
                     /locus_tag="ACHLAM_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO7853638.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2117..2428
                     /gene="rpsJ"
                     /locus_tag="ACHLAM_002113"
     CDS             2117..2428
                     /gene="rpsJ"
                     /locus_tag="ACHLAM_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO7853639.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2461..3090
                     /gene="rplC"
                     /locus_tag="ACHLAM_002114"
     CDS             2461..3090
                     /gene="rplC"
                     /locus_tag="ACHLAM_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO7853640.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3101..3706
                     /gene="rplD"
                     /locus_tag="ACHLAM_002115"
     CDS             3101..3706
                     /gene="rplD"
                     /locus_tag="ACHLAM_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO7853641.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3703..4005
                     /gene="rplW"
                     /locus_tag="ACHLAM_002116"
     CDS             3703..4005
                     /gene="rplW"
                     /locus_tag="ACHLAM_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO7853642.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4023..4844
                     /gene="rplB"
                     /locus_tag="ACHLAM_002117"
     CDS             4023..4844
                     /gene="rplB"
                     /locus_tag="ACHLAM_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO7853643.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4861..5139
                     /gene="rpsS"
                     /locus_tag="ACHLAM_002118"
     CDS             4861..5139
                     /gene="rpsS"
                     /locus_tag="ACHLAM_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO7853644.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5154..5486
                     /gene="rplV"
                     /locus_tag="ACHLAM_002119"
     CDS             5154..5486
                     /gene="rplV"
                     /locus_tag="ACHLAM_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO7853645.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5504..6205
                     /gene="rpsC"
                     /locus_tag="ACHLAM_002120"
     CDS             5504..6205
                     /gene="rpsC"
                     /locus_tag="ACHLAM_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO7853646.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6218..6628
                     /gene="rplP"
                     /locus_tag="ACHLAM_002121"
     CDS             6218..6628
                     /gene="rplP"
                     /locus_tag="ACHLAM_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO7853647.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6628..6819
                     /gene="rpmC"
                     /locus_tag="ACHLAM_002122"
     CDS             6628..6819
                     /gene="rpmC"
                     /locus_tag="ACHLAM_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO7853648.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6819..7073
                     /gene="rpsQ"
                     /locus_tag="ACHLAM_002123"
     CDS             6819..7073
                     /gene="rpsQ"
                     /locus_tag="ACHLAM_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO7853649.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7238..7609
                     /gene="rplN"
                     /locus_tag="ACHLAM_002124"
     CDS             7238..7609
                     /gene="rplN"
                     /locus_tag="ACHLAM_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO7853650.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7620..7934
                     /gene="rplX"
                     /locus_tag="ACHLAM_002125"
     CDS             7620..7934
                     /gene="rplX"
                     /locus_tag="ACHLAM_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO7853651.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7949..8488
                     /gene="rplE"
                     /locus_tag="ACHLAM_002126"
     CDS             7949..8488
                     /gene="rplE"
                     /locus_tag="ACHLAM_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO7853652.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8503..8808
                     /gene="rpsN"
                     /locus_tag="ACHLAM_002127"
     CDS             8503..8808
                     /gene="rpsN"
                     /locus_tag="ACHLAM_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO7853653.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8842..9234
                     /gene="rpsH"
                     /locus_tag="ACHLAM_002128"
     CDS             8842..9234
                     /gene="rpsH"
                     /locus_tag="ACHLAM_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO7853654.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9247..9780
                     /gene="rplF"
                     /locus_tag="ACHLAM_002129"
     CDS             9247..9780
                     /gene="rplF"
                     /locus_tag="ACHLAM_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO7853655.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9790..10143
                     /gene="rplR"
                     /locus_tag="ACHLAM_002130"
     CDS             9790..10143
                     /gene="rplR"
                     /locus_tag="ACHLAM_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO7853656.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10158..10661
                     /gene="rpsE"
                     /locus_tag="ACHLAM_002131"
     CDS             10158..10661
                     /gene="rpsE"
                     /locus_tag="ACHLAM_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO7853657.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10665..10844
                     /gene="rpmD"
                     /locus_tag="ACHLAM_002132"
     CDS             10665..10844
                     /gene="rpmD"
                     /locus_tag="ACHLAM_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO7853658.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10848..11282
                     /gene="rplO"
                     /locus_tag="ACHLAM_002133"
     CDS             10848..11282
                     /gene="rplO"
                     /locus_tag="ACHLAM_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO7853659.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11290..12621
                     /gene="secY"
                     /locus_tag="ACHLAM_002134"
     CDS             11290..12621
                     /gene="secY"
                     /locus_tag="ACHLAM_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO7853660.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12653..12769
                     /gene="rpmJ"
                     /locus_tag="ACHLAM_002135"
     CDS             12653..12769
                     /gene="rpmJ"
                     /locus_tag="ACHLAM_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO7853661.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12916..13272
                     /gene="rpsM"
                     /locus_tag="ACHLAM_002136"
     CDS             12916..13272
                     /gene="rpsM"
                     /locus_tag="ACHLAM_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO7853662.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13289..13678
                     /gene="rpsK"
                     /locus_tag="ACHLAM_002137"
     CDS             13289..13678
                     /gene="rpsK"
                     /locus_tag="ACHLAM_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO7853663.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13712..14332
                     /gene="rpsD"
                     /locus_tag="ACHLAM_002138"
     CDS             13712..14332
                     /gene="rpsD"
                     /locus_tag="ACHLAM_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO7853664.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14358..15347
                     /gene="rpoA"
                     /locus_tag="ACHLAM_002139"
     CDS             14358..15347
                     /gene="rpoA"
                     /locus_tag="ACHLAM_002139"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO7853665.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15388..15771
                     /gene="rplQ"
                     /locus_tag="ACHLAM_002140"
     CDS             15388..15771
                     /gene="rplQ"
                     /locus_tag="ACHLAM_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO7853666.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15878..16246
                     /locus_tag="ACHLAM_002141"
     CDS             15878..16246
                     /locus_tag="ACHLAM_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO7853667.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16257..16682
                     /gene="zntR"
                     /locus_tag="ACHLAM_002142"
     CDS             16257..16682
                     /gene="zntR"
                     /locus_tag="ACHLAM_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO7853668.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16738..16956
                     /gene="arfA"
                     /locus_tag="ACHLAM_002143"
     CDS             16738..16956
                     /gene="arfA"
                     /locus_tag="ACHLAM_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO7853669.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16953..17363)
                     /gene="mscL"
                     /locus_tag="ACHLAM_002144"
     CDS             complement(16953..17363)
                     /gene="mscL"
                     /locus_tag="ACHLAM_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO7853670.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17493..18869)
                     /gene="trkA"
                     /locus_tag="ACHLAM_002145"
     CDS             complement(17493..18869)
                     /gene="trkA"
                     /locus_tag="ACHLAM_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO7853671.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18891..20180)
                     /gene="rsmB"
                     /locus_tag="ACHLAM_002146"
     CDS             complement(18891..20180)
                     /gene="rsmB"
                     /locus_tag="ACHLAM_002146"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO7853672.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLNWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPPADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20226..21173)
                     /gene="fmt"
                     /locus_tag="ACHLAM_002147"
     CDS             complement(20226..21173)
                     /gene="fmt"
                     /locus_tag="ACHLAM_002147"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO7853673.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21188..21697)
                     /gene="def"
                     /locus_tag="ACHLAM_002148"
     CDS             complement(21188..21697)
                     /gene="def"
                     /locus_tag="ACHLAM_002148"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO7853674.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21827..22951
                     /gene="dprA"
                     /locus_tag="ACHLAM_002149"
     CDS             21827..22951
                     /gene="dprA"
                     /locus_tag="ACHLAM_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO7853675.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22923..23396
                     /gene="smg"
                     /locus_tag="ACHLAM_002150"
     CDS             22923..23396
                     /gene="smg"
                     /locus_tag="ACHLAM_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO7853676.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23425..23967
                     /locus_tag="ACHLAM_002151"
     CDS             23425..23967
                     /locus_tag="ACHLAM_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO7853677.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23972..24544
                     /gene="tsaC"
                     /locus_tag="ACHLAM_002152"
     CDS             23972..24544
                     /gene="tsaC"
                     /locus_tag="ACHLAM_002152"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO7853678.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24549..25367
                     /gene="aroE"
                     /locus_tag="ACHLAM_002153"
     CDS             24549..25367
                     /gene="aroE"
                     /locus_tag="ACHLAM_002153"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO7853679.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25364..25621
                     /locus_tag="ACHLAM_002154"
     CDS             25364..25621
                     /locus_tag="ACHLAM_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO7853680.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25597..26151)
                     /locus_tag="ACHLAM_002155"
     CDS             complement(25597..26151)
                     /locus_tag="ACHLAM_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO7853681.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
     gene            complement(26195..>26407)
                     /locus_tag="ACHLAM_002156"
     CDS             complement(26195..>26407)
                     /locus_tag="ACHLAM_002156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853682.1"
                     /translation="EFQDFFSLLLRPSCEMFHVVVSTEAHYRDPNFLHKYFFDLFFCL
                     LLFHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         6713 a         6248 c         6907 g         6539 t
ORIGIN      
        1 agagaagtta ttctttcctg gttgcgtgag aaacgtcaga cctggcgtct gtgctatttg
       61 ttgggtgagg ctggaagtgg aaaaacctgg ctggcgcagc aactacaaaa agataaacat
      121 cgccgtgtga ttactttaag cctcgttgtt tcctcgcaag gtaaggccgc atggatcgtt
      181 accgacgata atgcggctga acagggctgc cgtgacagcg cctggacgcg agatgagatg
      241 gcgggtcaat tactgcatgc gcttcatcga actgatagtc gctgccctct tattattatt
      301 gaaaatgctc acctgaatca ccgccgcata cttgatgatc tccaacgcgc gatatctctt
      361 attcctgacg gtcaattttt gctgatcggc agacccgatc gaaaagtcga acgtgatttt
      421 aaaaaacagg gcattgaact tgtctcaaca ggacgtctga tggagcacga gcttaaggca
      481 agcatccttg aaggtcagaa tattgaccag cccgatctcc ttttaactgc cagagttctg
      541 aaacggatag ctttattatg tcggggagat cgcagaaagc tggcgcttgc cggcgaaaca
      601 attagcttat tacagcaagc cgagcagacc agcgttttca ctgcaaaaca ttggcgaatg
      661 atttaccgcg tattaggtga taaccgcccc cggaaaatgc agcttgccgt ggtgatgagt
      721 ggaaccatta tcgcacttac ctgtggttgg ctactgctgt cctctttcac agatccgttc
      781 cccgttcctg cgtggctggt acccgtaacg ccagtagtta aacaagatat gactaaggat
      841 attgctcatg tagtgatgcg cgatagtgag gcgctaagcg tgttatacgg cgtatggggg
      901 tacgaggtcc cagctgattc ggcctggtgc gatcaggcag taagagcggg gctggcatgt
      961 aaaagtggga atgcttcttt acaaacattg gtagatcaga atttgccctg gatcgcatcg
     1021 cttaaagtcg gggacaaaaa attgcctgtt gtggtcgttc gcgtcggtga tgctaccgtt
     1081 gatgtcctcg tagggcagca aacctggacg ctgacacatc aatggtttga gtcggtatgg
     1141 acgggtgatt atcttctgtt atggaaaatg tcgccggaag gggagagtac cattacgcgt
     1201 gatagcagcg aggaagagat tctctggctg gaaacgatgt taaaccgcgc attgcatatt
     1261 tcgacagaac cttcggcaga gtggcgtccc ttgttggtag aaaaaataaa gcaatttcaa
     1321 aaaagccacc acttgaaaac tgacggtgtt gttggctttt caacgttagt acatctctgg
     1381 caggtggcag gggagagtgc ctacttatat cgggatgaag caaatatttc cccggaaaca
     1441 acagtgaagg ggaaatgaca tgtttgaatt ctatatcgcc gcgcgtgagc agaaagaaac
     1501 aggtcaccct ggtatcttct cccgacaaaa acacagcacc ataatatatg tcatttgttt
     1561 attgctgatt tgcctgtggt ttgcaggaat ggtgttggtt ggtgggtatg ccaggcagct
     1621 atgggcgctt tggatagtaa aagcagaagt cactgtagag gctgaaacgc ccgcttttaa
     1681 acaatcaaca caacactatt tcttcaaaaa acagccactc cccgtcgtcg aatccgtcga
     1741 ggaggaagat gacccagacg tcgcggttga gaatgctccc tcttcttctg aagacgaaga
     1801 aaatactgta gaagaaagcg acgagaaagc cggtttaaga gagcgtgtca aaaatgcgct
     1861 gaacgaactt gagagataac ccgaaggctg tttactttac tagcaatacg cttgcgttcg
     1921 gtggttaagt atgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     1981 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2041 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2101 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2161 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2221 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2281 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2341 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2401 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2461 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2521 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2581 gctaacgatg gctaccgtgc tattcaggta accaccggtg ctaaaaaagc taaccgtgtg
     2641 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2701 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     2761 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     2821 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     2881 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     2941 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3001 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3061 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3121 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3181 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3241 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3301 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3361 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3421 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3481 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3541 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcacaaggt
     3601 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3661 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3721 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     3781 tctaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     3841 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     3901 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     3961 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4021 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgtt gttaaagtgg
     4081 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4141 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4201 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4261 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttttgtac aaagacggtg
     4321 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4381 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4441 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4501 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4561 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4621 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4681 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4741 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     4801 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     4861 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     4921 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     4981 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5041 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5101 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5161 ctatcgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5221 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5281 cggctgtact ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5341 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5401 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5461 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5521 tcctaatggt attcgcctgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5581 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5641 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5701 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     5761 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     5821 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     5881 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     5941 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcagaaatcg cacgtaccga
     6001 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6061 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6121 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6181 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6241 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6301 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6361 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6421 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6481 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6541 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6601 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcgtt
     6661 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6721 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     6781 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     6841 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     6901 ttgtgaaaca cccgatctac ggtaaattca tcaagcgtac gaccaaactg cacgtacatg
     6961 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7021 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7081 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7141 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7201 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7261 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7321 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7381 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7441 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7501 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7561 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7621 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7681 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattgttgaa ggtatcaacc
     7741 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     7801 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     7861 accgtgtagg ctttagattc gaagacggta aaaaagtccg tttcttcaag tctaacagcg
     7921 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     7981 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8041 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8101 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8161 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8221 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8281 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8341 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8401 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8461 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8521 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8581 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8641 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8701 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     8761 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     8821 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     8881 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     8941 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9001 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9061 agcattcagc gtgtcagccg cccaggtctg cgcatctata agcgtaaaga tgagctgccg
     9121 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9181 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9241 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9301 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9361 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9421 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9481 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9541 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9601 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9661 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9721 ggcaagggtg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
     9781 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
     9841 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
     9901 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
     9961 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10021 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10081 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10141 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10201 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10261 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10321 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10381 cgctgaataa cggtactctg caacaccctg ttaaaggtgt tcatacgggt tctcgcgtat
    10441 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10501 tggaagtcgc tggggttcat aacgttctgg ctaaagcgta tggttccacc aacccgatta
    10561 acgtggttcg tgcaactatc gatggcctgg aaaatatgaa ttccccagaa atggtcgctg
    10621 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10681 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10741 gcctgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    10801 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    10861 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    10921 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaaatctcgt tctggcggtg
    10981 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11041 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctgtctgac ctggctaaag
    11101 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11161 tcgagttcgc gaaagtgatc ctggctggcg aagtcactac tccggtaact gttcgtggcc
    11221 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11281 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11341 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11401 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11461 ggcaccatca ttgaaatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11521 tttgctctgg ggatcatgcc atatatttcg gcatcgatca ttatccagct tctgacggtg
    11581 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11641 cagtacaccc gctacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11701 ggtctgccga atatgcctgg tatgcaaggc ctggtcatta acccgggctt tgcattctac
    11761 ttcaccgctg ttgtaagtct ggtcacagga accatgttcc tgatgtggtt gggcgaacag
    11821 attactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    11881 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    11941 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtatttgtt
    12001 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12061 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12121 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12181 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12241 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12301 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12361 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cctggttggt
    12421 gcgctgtaca ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12481 ccgttctact ttggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12541 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12601 ctgaaaggct acggccgata attggtcgcc tgagaagtta cggagagtaa aaatgaaagt
    12661 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12721 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    12781 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    12841 gtgcgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    12901 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    12961 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13021 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13081 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13141 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13201 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13261 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13321 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13381 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13441 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13501 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13561 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13621 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13681 cttcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13741 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    13801 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    13861 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    13921 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    13981 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14041 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14101 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14161 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacctg
    14221 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14281 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14341 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14401 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14461 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14521 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14581 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14641 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14701 tcacccacga cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    14761 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    14821 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    14881 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    14941 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15001 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15061 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15121 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15181 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15241 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15301 gcatgcgcct ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15361 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15421 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15481 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15541 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15601 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15661 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15721 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    15781 acgtaaaaaa acccgccccg gcgggttttt ttatacccgt agcatcccca cttatctaca
    15841 atagctgtac tctttttgtt catcccctgg agtatatatg tggttacttg accagtgggc
    15901 agagcgtcat atagcagaag cgcaagcgaa aggtgagttt gataacctgg caggtagcgg
    15961 cgaaccattg atactggctg atgattctca tgtgccaccg gaattacgtg cggggtatcg
    16021 cttgctgaag aatgccggtt gcttaccgcc agaacttgag cagcggaggg aagcaattca
    16081 gcttctggat atcctgagag gtatccgtca cgatgatccg caatatcaag aggttagccg
    16141 tcgattgtca ttactggaat tgaagctgcg acaagctgga ctgagcaccg attttttacg
    16201 cggcgattat gctgacaagt tgttgaataa aatcaacgat aactagtgga gtatgtatgt
    16261 atcgcattgg tgagctggca aaaatggcgg aagtaacacc cgacacgatt cgttattacg
    16321 aaaaacagca gatgatggag catgaagtgc gtactgaagg tgggtttcgc ctgtataccg
    16381 aaagcgatct ccagcgattg aaatttatcc gccatgccag acaactaggt ttcagtctag
    16441 agtcgatccg cgagttgctg tcgatccgta tcgatcctga acaccatacc tgtcaggagt
    16501 caaaaggcat tgtgcaggaa agattgcagg aagtcgaagc acggatagcc gagttgcaga
    16561 gtatgcagcg ttccctccaa cgccttaacg atgcctgttg tgggaccgcc catagcagtg
    16621 tttattgctc gattcttgaa gctcttgaac aaggggcgag tggcgttaag agtggctgtt
    16681 gattttttac actggcagga ttacactcgc gccgttaaat aaccaattgg agtttttatg
    16741 agccgatatc agcatactaa agggcagata aaggataatg cgatagaagc attactacat
    16801 gatcccttat tccgacagcg cgtagagaga aataagaaag ggaaaggcag ttacatgcga
    16861 aaaggaaaac atggcaatcg ggggaactgg gaggccagtg gcaagaaagt aaatcacttt
    16921 tttaccactg gccttctgct ttcaggcgct tgttaagagc ggttattctg ctctttcagc
    16981 aaatcacgaa tttctgtcag taatacttct tctttagttg gtgtaggcgc ggctgctggt
    17041 tcttcttttt tccgattcag tttgttgatt agcttaatcg ccataaagat ggcaaaggct
    17101 acaatcagaa aatcaaagac gttttgaatg aagacaccgt aatgcatcac aacagcaggg
    17161 atatccccct gcgcatcgcg tagcgtgaca gcaaactgtt tgaaatcgat cccgccaatt
    17221 aataacccca gaggtggcat aatgatatcg gcaaccagtg aagatacaat cttcccgaat
    17281 gccgcaccga taatgacacc caccgccaaa tccaccacgt tcccgcgcat cgcaaattcg
    17341 cgaaattctt taataatgct catgttattc tccctatgaa gccgacaatc ataagtctaa
    17401 caaatgttaa gctattttcc tgccaggagt gaatttaatt aatccctgat aatcaaaggg
    17461 aaataataag gcgtcattag acgccttatt aattacaaga agaaaggact tggctggaag
    17521 agtctttcga cgtcggtaat aaacttttta tctgtgagga acataattac gtgatcgcct
    17581 tgctcaatgc gcagattgtc attggcaatc atcacgtcgt ttccacgtac caccgctcca
    17641 ataatcgttc ctggcggtag cttgatttca tcaatgactc tgccgacaac gcgtgaggtg
    17701 ctttcatcac cgtgagcaac ggcttcaata gcttctgcta cgccgcggcg cagtgaggaa
    17761 acaccaacaa tatcagcttt tcgcacatgg ctaagcaacg cagaaatagt tgcttgttgc
    17821 ggtgaaatcg caatatcgat cacgctcccc tgaaccagat ccacataagc gcggcgctgg
    17881 atcaatacca tcaccttttt cgcacccata cgtttggcaa gcatggcgga catgatattg
    17941 gcttcgtcat cgttggtgac agcaataaac agatcaactt gatcgatatg ttcttcggcc
    18001 agcagttctt gatccgacgc atcaccaaaa aagacgatcg tattctgcaa cttttccgcc
    18061 agttcggcag cgcgctgctg attacgttcg atgagtttaa cgctgtaatc tttttccaga
    18121 cgacgcgcca gccctgcacc gatattaccg ccgccaacca gcatgatccg cttatacggt
    18181 ttttccagtc gctgtaattc actcatcacc gcgcggatat gctgtgaagc ggcaataaag
    18241 aacacttcat caccagcttc aacaatggtc gaaccttgcg gacgaatggg gcgatcgtgg
    18301 cggaaaattg ctgccacacg agtatcgata tggggcatat gttcgcgcat ggtcgaaagt
    18361 gcattaccaa tcagcgggcc gccataatag gctttaacca cagccaggct gactttaccc
    18421 tcagcgaagt tcaccacctg caatgcgccg ggatactcaa tcagtcggta aatattatcg
    18481 ataaccaact gctctggtgc gatcagatga tcaatcggta cagcatctga atgaaatagt
    18541 ttatcggcat cgcgcacgta gtctggtgag cggatacgag cgatgcgatt aggggtgttg
    18601 aaaagtgagt aggctacctg gcaggcaacc atattggttt catctgaact agttacagca
    18661 accagcatat cggcgtcgtc ggcacctgcc tcccgcaata cgcgtggatg agagccatgc
    18721 ccctgcacga ctcgcaggtc aaatttatcc tgcaaggtcc gcagacgctc accgttggta
    18781 tcgacaacag taatatcgtt gttctcgcca accaggtttt ccgccagtgt gccgccaacc
    18841 tggccggcac ccagaatgat aattttcatc agtcgcgacc cgttatctca tcactttttg
    18901 attagcttag cgtaaaagaa gccgtcgccc tcttcggcac caggcagatt ttgtttaccc
    18961 ggttgctctg gtgttcctgt ttcgcaaagt tcggcatcag cggtacgttg caaaaaggct
    19021 ttaatctgca ggctattctc ttccggtaac accgaacagg tggcatagac cagggttcca
    19081 ccggatttta aatgcggcca aatggcgtcg agaatttcag actgcaattg cgcgagttcc
    19141 gggatatcgc gatcgcgacg taaccattta atatccggat ggcgacgaat cacaccggtt
    19201 gctgaacaag gcgcatctaa taaaatgcga tcaaactgtt gctcgccaca ccattgggaa
    19261 gggtagcggc catcaccttg tttcacggtt gccttcatac caaggcgttt taaattgtcg
    19321 taaacgcgag agaggcgctg ttcgtcaata tcaaccgcaa caacctgcgc ttctggtgcc
    19381 acctcaagga tatgcgttgt tttaccgccg ggggcggcac aaagatccaa aatgtgttca
    19441 ccgttttgtg gcgcaagcca ggtcatgcaa ccttgtgctg aggcatcctg aacggtaacc
    19501 catccgtctt caaaaccagg tagcgcatga acaggtgcag gtgtttccag acgtacagca
    19561 tcagggtaat ccgcaggcgg gaaacctttc attcctgctt catccagcaa tgcaagccag
    19621 ctgtcgcggg aatgatgcgt acgattaacg cgcagccaca ttggcggacg ctggttattg
    19681 gcttcgacga tggattgcca ctgctctgga tacgcttttt gcagacgctt cagcaaccag
    19741 gaaggatgca gataacgtgc atcactggca ttaaactcgg ctaataactc ttcttgctga
    19801 cgctggaact ggcgtaatac accgtttatc aaccctttaa gttgcgggcg cttaattgcg
    19861 atagcgcctt caaccgtttc agccagcgca gcatgaggtg gtatgcgggt ataaagcagt
    19921 tgatacaaac caaccataat caggtaatgc acggtccgct gtttgccggt catcggacgg
    19981 gccattaact tattaatcag ccagtttaac tgcgaaagcg tacgcagtac gccaaagcac
    20041 aactcttgaa gaagtgcttt gtctttatcg gaaacttttt gctggagcgg tggcagaatg
    20101 ttgcttaatg attgcccttg ctcgacgact tgttcaacgg cctgggccgc catgctacgt
    20161 aaattacgtt gttttttcat aagtataaaa atgcccggca agaccgggct tagaagagtg
    20221 gactatcaga ccagacggtt gcccggaaca aaccattccc gacgagagtt caggaggtct
    20281 tgcgcgctca tcgctttctt acccgcaggt tgtaacgaga gcaggttcag gatgccatca
    20341 ccagtcgcaa cctgaatgcc ttgtttgttg gcttcaagga tcgttcctgg tgcagcgttg
    20401 gttgccgtat caatgaccga tgctttccag actttaaccg gctgtccttc aatttccagc
    20461 cagctcattg gccatggatt gaaagcgcga atgcagcgtt caagctgtgc tgccgaaagt
    20521 gaccagtcaa tacgcgcttc ttctttactc aacttctcgg cgtaagtgac aagagtttcg
    20581 tcctgaactt ctggtttcgc cgtgccgtct gccaattgtt tcaacgtggt gataagccct
    20641 tgtgggccaa gctctgccag cttgtcgtac agcgtaccac tggtatcttc tgcagtaatc
    20701 gggcaggaga gcttatagag catgtctccg gtgtctaaac caacatccat ttgcataatg
    20761 gtcacaccag tttctgcatc acccgcccat agtgagcgtt ggattggtgc agcaccgcgc
    20821 cagcgtggca gcagtgaacc atgaacgttg atacagccaa gacgcggcat ctccagcact
    20881 gctttaggca gaattaagcc ataggcgacg acaaccataa catcagcctg tagatcggcg
    20941 accagttgct ggttttcttg tggacgcagg gaaaccggtt gaaaaacggg cagacctttt
    21001 tcctcagcca gaactttaac cgggctgggc atcagttttt taccgcgtcc tgccggtcgg
    21061 tctggctggg tgaacacgcc aacgacgtta tgaccagaag acaacagcgc gtcgagatga
    21121 cgcgctgcaa agtcaggtgt acccgcaaaa ataatacgta gtgattctga cacgttagtt
    21181 cttatcctta agcccgggct ttcagacgat ccagtttttc aactttctga cgaatacgtt
    21241 gttgtttcag cggtgacaga taatccataa acagtttgcc gaccaggtga tccatctcat
    21301 gctgaataca gatggctaac agaccctctg cttccagttc aaatggttta ccgtcgcggt
    21361 ccagggcgcg aattttaact ttctctgcgc gtggcactaa agcacgttgt tcagggatcg
    21421 acaggcatcc ttcttcaatg cctgtttcgc cgcttttttc taaaagctcc ggattgatta
    21481 acaccagccg ttcgtcacgg ttttccgaaa catcaataac aatgatacgt tgatggatat
    21541 cgacctgggt tgccgccagg ccaatacctt cttctgcgta catcgtctcg aacatatcat
    21601 cgacgatacg ctgaatttct gcattcactt cttctaccgg tttagcaact ttgcgaagcc
    21661 gctcgtccgg aatatgtaac acttgcaaaa ctgacataaa tctccagaga tgtgttcagg
    21721 agttagaaag attatttctt ctattctaga caaatccccc tctgattgac agcatcactg
    21781 accaatcgca aagattgcta aggctgctta tggcagggag ataaggatgg tcgatacaga
    21841 aatttggctg cgtttaatga gcatcagcag cttgtacggc gatgatatgg tccgtatagc
    21901 tcactggctg gcaaaacagt cgcatattga tgcggttgta ttgcagcaaa ctgggcttac
    21961 attgcggcag gcacaacgct ttctttcatt tccgcgaaag agtatcgaaa gctcactttc
    22021 ttggttggag caacccaacc atcatttaat tcctgcggac agcgaatttt atcctcctca
    22081 acttcttgcg acgacagatt accccggcgc actgtttgtt gaaggagaac tgcacgcgct
    22141 gcattcattt cagcttgccg tagtggggag tcgggcgcat tcatggtatg gcgagcgatg
    22201 gggacgatta ttttgcgaaa ctctggcgac gcgtggagtg acaattacga gtggactggc
    22261 gcgtggaatc gatggtgtgg cgcataaagc ggccttacag gtaaatggcg tcagcattgc
    22321 tgtattgggg aatggactta ataccattca tccgcgtcgc catgcccgac tggctaccag
    22381 tttgcttgaa catggtgggg ctcttgtctc ggaatttccc ctcgatgttc caccccttgc
    22441 ttacaatttc ccacgaagaa atcgcattat cagtggtcta agtaaaggtg tactggtggt
    22501 ggaagcggct ttgcgcagtg gttcgctggt gacagcacgt tgtgcgcttg agcaggggcg
    22561 tgaagttttt gccttgccgg gtccattagg gaatccggga agcgaagggc ctcactggtt
    22621 aataaaacaa ggtgcgattc ttgtgacgga accggaagaa attctggaaa acttgcaatt
    22681 tggattgcac tggttgccag acgcccctga aaattcattt tattcaccag atcaggaaga
    22741 cgtggcattg ccatttcctg agctcctggc taacgtagga gatgaggtaa cacctgttga
    22801 cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta gtaactcaac tactcgaact
    22861 ggagttagca ggatggatcg cagctgtacc cggcggctat gtccgattga ggagggcatg
    22921 ccatgttcga cgtactaatg tatttgtttg aaacctatat tcacacagaa gctgagttgc
    22981 gtgtggatca agacaaactt gaacaggatc ttaccgacgc aggatttgat cgagaagata
    23041 tctacaatgc cctgctttgg ctggaaaagc ttgcggatta tcaggaagga ctggcagaac
    23101 cgatgcaact ggcctctgat ccactctcca tgcgtattta tacccctgaa gagtgtgaaa
    23161 ggctggatgc cagctgtcgt ggttttctgc ttttccttga gcaaattcag gtgcttaacc
    23221 ttgaaacccg tgaaatggtg atagagcgag tgctcgcgct ggataccgca gagttcgacc
    23281 tggaagacct gaaatgggta atcctgatgg tgttgttcaa tattccgggc tgcgaaaatg
    23341 cgtaccagca aatggaagaa ttactctttg aagtgaatga aggtatgctg cattaatcat
    23401 ttctttaatt cagcataagt tgttatggcg aaatcagcac tgttcacggt gcgtaataat
    23461 gagtcctgcc caaagtgcgg ggctgaactg gttattcgat ccgggaaaca cggtccgttt
    23521 cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc ctctgaaatc ttcagcggat
    23581 ggacatattg tcaaagttct ggaggggcag gtttgccctg catgtggcgc aaatctggta
    23641 ttacgccagg gacgctttgg tatgtttatt ggttgcagta actaccctga atgcgaacat
    23701 accgaactta tcgataaacc ggacgaaaca gcaattacct gtccccaatg tcggacgggc
    23761 catctggtcc agcgccgttc ccgttatggc aaaacatttc actcctgtga tcgctacccg
    23821 gagtgtcaat ttgccattaa cttcaaaccc atagctggag aatgccctga gtgtcattat
    23881 ccgctactca tcgaaaagaa aaccgcgcag ggtgtaaaac acttttgtgc cagtaaacaa
    23941 tgtggaaagc cggtttcggc ggaataataa cgtgaataat aacctgcaag gagacgctat
    24001 cgcagctgcg atagatgtcc tcaatgaaaa acgtgtcatc gcctatccaa cggaagccgt
    24061 tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg atgcgactgt tggagttaaa
    24121 acagcgtccg gttgataagg ggctgatttt aatcgcggca aattacgagc agcttaaacc
    24181 ctatattgat gacaccatgc tgactgatgc gcagcgtgaa accatttttt cccgctggcc
    24241 aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca ccgcgctggt tgacgggccg
    24301 ctttgattcg cttgctgtac gagtcaccga ccatccgttg gtggttgctt tgtgccaggc
    24361 ttatggtaaa ccgctggttt ctaccagtgc caacttgagt ggattgccac cttgtcgaac
    24421 agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg gttgtgcctg gtgaaacggg
    24481 ggggcgttta aatccttcag aaatccgcga tgccctgacg ggtgaactgt ttcgacaggg
    24541 gtaacataat ggaaacctat gctgtttttg gtaatccgat agcccacagc aaatcgccat
    24601 tcattcatca gcaatttgct cagcaactga atattgaaca tccctatggg cgcgtgttgg
    24661 cacccatcaa tgatttcatc aacacactga acgctttctt tagtgctggt ggtaaaggtg
    24721 cgaatgtgac ggtacctttt aaagaagagg cttttgccag agcggatgag cttactgaac
    24781 gggcagcgtt ggctggtgct gttaataccc tcaagcggtt agaagatgga cgcctgctgg
    24841 gtgacaatac cgatggtata ggtttgttaa gcgatctgga acgtctgtct tttatccgtc
    24901 ctggtttacg tattctgctc atcggcgctg gtggagcttc tcgcggcgta ctactgccac
    24961 ttctttccct ggactgtgcg gtgacaatca ctaatcggac ggtatcccgc gcggaagagt
    25021 tggctaaatt gtttgcgcac actggcagta ttcaggcgtt gggtatggac gaactggaag
    25081 gtcatgagtt tgatctcatt attaatgcaa catccagtgg catcagtggt gatattccgg
    25141 cgatcccgtc atcgcttatt catccaggca tttattgcta tgacatgttc tatcagaaag
    25201 gaaaaactcc ttttctggca tggtgtgagc agcgaggctc aaagcgtaat gctgatggtt
    25261 taggaatgct ggtggcacag gcggctcatg ccttccttct ttggcatggc gttctgcctg
    25321 atgtagaacc ggtcatcaaa ctattgcaac aggaattgtc agcgtgaatc aggccatcca
    25381 gtttccggat agggaagagt gggacgagaa taaaagatgt gtatgttttc ccgctctcgt
    25441 gaatggtatg caactgacat gtgcgatctc tggcgagagt ctggcgtatc gctttactgg
    25501 agatacgcca gaacagtggt tagcgagttt tcgtcagcat cgctgggacc tggaagaaga
    25561 agcggaaaac ttaattcagg aacaaagtga agatgatcaa ggttgggtct ggttaccctg
    25621 atccagatat tcgtccttcc atttcacgta attcttcgcg gaatagcgta acccagcctt
    25681 ctcttcatca cttaacgggc ggatctgttt gacggggcta ccgagataca gatatccgct
    25741 ctccagccgt ttattttgtg ggaccaggct acccgcacca atcatcacat catcttctac
    25801 tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa actcgattgc caatggtgca
    25861 gccgtggagc atcaccttgt gaccaacagt gacatcttcg ccaatggtta atgggttgcc
    25921 atctgggttg tacgaggatt tatgagtgac atgcaacata ctgccatcct ggatattggt
    25981 gcgtgctccg atctgtacat aatgtacatc tccacgaatc acaacgagcg gccagatccc
    26041 cacatcatca gccagacgaa cgtcaccaat cacgacgctg ctatcgtcga tcattacgcg
    26101 ctggccgatt tgtggaaaaa gatcgcggta tgggcgtaaa acatcagaca tactcacctc
    26161 aacaataaat gatttactaa tgactttggg tgcattattg gccttgtgca agtcttttag
    26221 catgcaaaaa agcaccgttt tgtgtgcgaa tgcagcaaaa agggtgaaaa agcaacaaac
    26281 agaaaaaaag atcaaaaaag tacttgtgca aaaaattggg atccctataa tgcgcctccg
    26341 ttgagacgac aacgtgaaac atttcacaag atggtcgtaa caacaaagag aaaaaatcct
    26401 gaaattc
//
LOCUS       DAZGAP010000062        26223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGAP010000062 DAZGAP010000000
VERSION     DAZGAP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.062"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(21..2093)
                     /locus_tag="ACHLAM_002157"
     CDS             complement(21..2093)
                     /locus_tag="ACHLAM_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO7853683.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     VHNPWGLAIVIAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPHIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMEFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDYAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            complement(2090..3631)
                     /locus_tag="ACHLAM_002158"
     CDS             complement(2090..3631)
                     /locus_tag="ACHLAM_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFO7853684.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWMSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(3641..4417)
                     /locus_tag="ACHLAM_002159"
     CDS             complement(3641..4417)
                     /locus_tag="ACHLAM_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO7853685.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4427..5269)
                     /locus_tag="ACHLAM_002160"
     CDS             complement(4427..5269)
                     /locus_tag="ACHLAM_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7853686.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(5279..6070)
                     /locus_tag="ACHLAM_002161"
     CDS             complement(5279..6070)
                     /locus_tag="ACHLAM_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO7853687.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            6283..6930
                     /locus_tag="ACHLAM_002162"
     CDS             6283..6930
                     /locus_tag="ACHLAM_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7853688.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(6914..7552)
                     /gene="ytfB"
                     /locus_tag="ACHLAM_002163"
     CDS             complement(6914..7552)
                     /gene="ytfB"
                     /locus_tag="ACHLAM_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO7853689.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7770..8390
                     /gene="fklB"
                     /locus_tag="ACHLAM_002164"
     CDS             7770..8390
                     /gene="fklB"
                     /locus_tag="ACHLAM_002164"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7853690.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8699..10111
                     /gene="cycA"
                     /locus_tag="ACHLAM_002165"
                     /gene_synonym="dagA"
     CDS             8699..10111
                     /gene="cycA"
                     /locus_tag="ACHLAM_002165"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO7853691.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(10156..10818)
                     /gene="ytfE"
                     /locus_tag="ACHLAM_002166"
     CDS             complement(10156..10818)
                     /gene="ytfE"
                     /locus_tag="ACHLAM_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO7853692.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(10926..11891)
                     /gene="ytfF"
                     /locus_tag="ACHLAM_002167"
     CDS             complement(10926..11891)
                     /gene="ytfF"
                     /locus_tag="ACHLAM_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7853693.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(11999..12859)
                     /gene="qorB"
                     /locus_tag="ACHLAM_002168"
     CDS             complement(11999..12859)
                     /gene="qorB"
                     /locus_tag="ACHLAM_002168"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7853694.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12948..13328
                     /gene="ytfH"
                     /locus_tag="ACHLAM_002169"
     CDS             12948..13328
                     /gene="ytfH"
                     /locus_tag="ACHLAM_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO7853695.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13457..15400)
                     /gene="cpdB"
                     /locus_tag="ACHLAM_002170"
     CDS             complement(13457..15400)
                     /gene="cpdB"
                     /locus_tag="ACHLAM_002170"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO7853696.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15590..16330
                     /gene="cysQ"
                     /locus_tag="ACHLAM_002171"
     CDS             15590..16330
                     /gene="cysQ"
                     /locus_tag="ACHLAM_002171"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO7853697.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAVAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16320..16877)
                     /gene="ytfJ"
                     /locus_tag="ACHLAM_002172"
     CDS             complement(16320..16877)
                     /gene="ytfJ"
                     /locus_tag="ACHLAM_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7853698.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            17202..17408
                     /gene="ytfK"
                     /locus_tag="ACHLAM_002173"
     CDS             17202..17408
                     /gene="ytfK"
                     /locus_tag="ACHLAM_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO7853699.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17470..18813)
                     /gene="paeA"
                     /locus_tag="ACHLAM_002174"
     CDS             complement(17470..18813)
                     /gene="paeA"
                     /locus_tag="ACHLAM_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO7853700.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19136..19774)
                     /gene="msrA"
                     /locus_tag="ACHLAM_002175"
     CDS             complement(19136..19774)
                     /gene="msrA"
                     /locus_tag="ACHLAM_002175"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO7853701.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            19980..21713
                     /gene="tamA"
                     /locus_tag="ACHLAM_002176"
     CDS             19980..21713
                     /gene="tamA"
                     /locus_tag="ACHLAM_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO7853702.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21710..25489
                     /gene="tamB"
                     /locus_tag="ACHLAM_002177"
     CDS             21710..25489
                     /gene="tamB"
                     /locus_tag="ACHLAM_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO7853703.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSMPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25492..25833
                     /gene="ytfP"
                     /locus_tag="ACHLAM_002178"
     CDS             25492..25833
                     /gene="ytfP"
                     /locus_tag="ACHLAM_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO7853704.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            26000..>26223
                     /locus_tag="ACHLAM_002179"
     CDS             26000..>26223
                     /locus_tag="ACHLAM_002179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853705.1"
                     /translation="MFDREMINENKEAFFLYVNGRGELSAQCVIKVAEKGDYLQGYSL
                     TRRAFRTFLKDRVIQMFSSDQELNKVASET"
BASE COUNT         6321 a         6643 c         6920 g         6339 t
ORIGIN      
        1 tacagaggaa atattgaaaa ttattgtgtg taaatgtctc cctttaaaaa atctggccag
       61 tttttttcaa agtaaatacc gttaatacac ggttgcatac gtaaagactt tacttcggta
      121 ataaaagaac cttgcgaata ttgaattgtt aatttgtgat ttttgtcttc agcgagttca
      181 gagcgaaaac gccattcttc ttttgcgccg cgacgagttt gtggtaggca tttgccttgt
      241 ggatcaataa ccatacgcgc cccacggcta ccaccaccag catcaatata atgcgttaat
      301 tgtgttaata ccgcggcaga agtcagcgcc atatgccgcc acataaacag ctcggcaact
      361 tcacccacat gtttgattgc caatccatgc cgttggacaa attcgttcaa tagcagggca
      421 tcacgagtgg cacgtcggac tttatcggca tggcaaataa atcccgcata gtcagacatt
      481 cgtgcctgaa ttttttctct cacaaccgac aacggcatcc cggtcccgtt atcgtgcgcc
      541 tgagtaatga tctctcttat agaagccagt gccggagcta ccagctgtgc tatatctcca
      601 tcaatattac gtttttgcgt gcaaccaata aaacgtgcca gacgaacagc aaaaacctgc
      661 ccggcattca atgccgcacc accagggcga gtgacgccgt gtgtgccagc aacttccccc
      721 acggcgaaac agccgggcag ggaggtttgt ccccagatat ccacttcaat gccgccattc
      781 atatgctgat tattcatggc aaactgcaaa ggctgtgtgg tgagatcgta accgtgcatc
      841 ttatacagcg agatagacag cggattcatt cgttgcagcc gttcgatggg cgatggtgcc
      901 agagcgtcgt tattttccag atacgcgcga acgtcgtcgt ccagtcgatc taatgagaag
      961 ggcaggtcac ccggcaccgc ttcaggattg cgattgaaat ccataaatac ctgacgccct
     1021 gattgctgct cttgcgctac agccatatct aacaagctgg agccaaactc cattacccga
     1081 gtggcgtgga acggccactg atagccttta cgaaaaatgt ttgaagccag ctcctgggtg
     1141 gtgcgatagt aatccgcgag gaagttatac tcgttgcccc cggcatccac ggaatagata
     1201 tgtgggatca cctgcacata ggtgccggac aaattccacg gaaacgtgct gcgcggtgtg
     1261 cctataccaa actggctttc agtcaaattg gttagcgtca gaccttcttc cagcgccagc
     1321 cccagcgagc cgaaacattt gtgtgggtac acactgtcgc gataaagctc gccaggccct
     1381 cctgttgcca gtaccacatt gggcgcaatc acaattgcca gcccccatgg gttatggacg
     1441 cgatgaccgg tcgcgaggat agcccccgcc acgcggtctt cgccgttttc gtcacgctga
     1501 tgcagcagtt taatcactgt tgcactggtc aacattggga tagcaagacg ctgtacttct
     1561 tccagcagca ctttcaccat taaccttgag gtacgtggcc cacacgaggt tgcacgcccg
     1621 gcttcgtcat ggtcggtttg ataacgaaga atcgcaccat agcgatcttc cggtaattcc
     1681 agaccaagat attgcaaccc gccaagagtg tgcagagaac ctaccgcttc gacataagca
     1741 gtgtcgtgat ccatcgcccc accactcgcc agtgcctctg ccagtttggt gaagttgtcg
     1801 ccgttgcccg cggtagcggc ggtaaacagc gtctgtttat cggaaccgga gcaggccgat
     1861 gttcccatgt ataacccggc ggtggcgatt aacacatttt gctggcggcg tttaagttcc
     1921 acagctgcac gcagcccggc tgcaccactg ccgactacca gggcatctgt ttgccaaagt
     1981 gggtaatcaa caccgtctac cgtgacaacc catgcgggtt ctgcctgtgc aaaacccttt
     2041 gggaaagtaa cgtgcgtcag cgcatcaagg atggtgtcat caagctgact catgcatcct
     2101 ccatcggcgt aaacaggcta ctttccatca ggcgtgggtg cttgataatt ggacggaaag
     2161 ccatatacgg cagaatgtcg cgctcaatct ccacacccgg cgcgatttca attaattcaa
     2221 cgccttctgc cgttaactgg aatactgcgc gttcagtgat atacagcact tcctgaccac
     2281 tttgctgtgc gtaggcggcg ctaaaagtga ttttttccac ttgggtaacc agtttaggga
     2341 tttgacctga ctgggcgata tgcaggccat caggcgttac atcaatcttg ctgcctttgg
     2401 cgtcaaacgt accgcagaac accactttac gggcgttttg cgcaatttcg aggaagccgc
     2461 cggggccaat gagattgcca ttgagatggg atacgttgac gttaccgtac cgatccatct
     2521 ctcccatgcc gaggaaggtg atatcgatac cgccgccact gtagaattcg aattgatcga
     2581 gtgatggaat aatggcgtcg gcgttacggg cgcagccgaa cagagcatca tccagcatca
     2641 tgccgttatg tacgccttgc tcgacgctca tccataaact ttgataaggc acgccttcgc
     2701 gcagcgcaac cccaggaata ccgcccggaa taccaaagcc aaagttggtt gaagcgcctg
     2761 ccaccagttc ccgtgctgcc cgacgggcaa tcaggcgacg caccggatga ctaaccagtt
     2821 caatagcgtc gttagaactt agacggcgat gttgaccgct gatcgtcagg tcgtaaccgc
     2881 caagataggt ttgcggttgt tcgcggtgtt caacgatacc atcgaccaga atccccggta
     2941 gcttgacctt gcgaggttca atggcgccag cttccagtac atcgcgcacc tgtacaaaca
     3001 ctttgccgcc actgttgtgt gctgccagcg ccatcgaata gctatccata tcaatcgctt
     3061 cttcttcgag gctaacgttg ccacgcggat cggcataggt gccgcgcaaa atcgcgtagt
     3121 ccactttgaa agggcgatag cggagtttgg tttcaccgtc gatttcaatc agttctacca
     3181 ggtcgtcggt cgtgcattca ttcgacttac cgccaccatt gcgtggatca acaaacgatc
     3241 ccagcccaac gtgggtaaaa agccccggac gtccggcacc gatctcccgc agcagtgcct
     3301 gaattacgcc accagggaaa cagtaggctt caatagcatt gcttttcacc agttcctgca
     3361 tcttgggcga ccaggtaaaa tgtccggcaa taatgcgttt gagcatttcg gcgtgagcaa
     3421 aacggttagt gcctttgctg tcgcgatcgc caatacccag cgagtggatt aatgtcagat
     3481 cgcgcgggtg tccggtttgc aggaaacgcg cttcaatggc ggccataata tggtcagctt
     3541 ccaccatacc gccgccgtta ccgctaatgg caatggttgc gccgtcctga atttgcgccg
     3601 ccagtgtctc ggcggttgat aacttacgca tacaatttcc ttattgattg tggaagctca
     3661 cgccggcttt ttcggcaaat gcgtgcgtcg cctgctggca atcctgagtg gcggacatta
     3721 atccagccag taactggtgg gagtaatcgg cgtgtttatt ttccagcgcc agaatcagtt
     3781 gcttaatatg tgccagtgcc accggaccac atttttccag ctgcgctaac tggttcgcca
     3841 tccagctttc cacctgttct ttttctacca cttcatcaat taaattcgcc acctgggctt
     3901 cctgcgccgt caggcgttta cccagcatca gcatctggcg accgaccacc gggccaacct
     3961 gattaagcac gcgttcaatg cccatccagc caggcaccat gccgagcatc acttccgggt
     4021 tagagaattt cgcgccgggg cgagcgatac gaatatcgca gcacaaggca agttcaatac
     4081 cgccaccgat ggtatgaccg ttgagattcg caacggttaa ttggggtaag ttgcgcaggc
     4141 gattaaaaac ctcgttaccg cgtttaatcc attttctgcc catatctaat ggcgaatagg
     4201 ccgaccacga tttaatatcg ccgcccgcac aaaagaattt atcgccggtt gccgttaatt
     4261 cgactaatcg taatgtggtg tcgttttcaa tttcatttaa ataatggtcg agagattcga
     4321 tcatttcttc attaatagca tgacatttat cttcacggtt caatgtcagg cggcaagaag
     4381 ccgccgctct gctgaacaga acgggttggt cactcatgat tattccttag caataatgtg
     4441 cgatgcgatc gacgctaata tccagcgtgc tgttaatatc tttctgccac cagtacggtg
     4501 agaatatttc cagttcaata gcgccattaa atccggcgtt ttcaaccagc cgacgaattg
     4561 acggaatatt gatgacacca tctcccggca ttccacggtc attgaccaga tcggtggtcg
     4621 gcactaacca gtcggaaaca tgaaacgcga ggatgcgttt tccggcacgc aggatctgac
     4681 tggcgagatc cgggtcccac cagacatggt agacatctac cgccacgccg aggccaaatt
     4741 cgccatcggg atcgagttca tcacaccagt ccagcgcctg acgtaacgtg cacagacaag
     4801 aacgatcggc agcagtcatc gggtgcagtg gctccagtgc aattggcacg ccgacctctt
     4861 tcgaatgtgg cagcaactgg cgaatccctt gtttcacctg ttcccgtgct tcgtacagat
     4921 cttttgttcc cattggcaga ccgccaacca cctgcatgta acaggcggca tttagcacag
     4981 cggcatcgtc cagcgcccgg cggttatcat caatcgccag cttgcgctcc gccagcgtcg
     5041 gtgcggtgta gtaggtgcta cggcacaaac cggaaacgtt tatgttactg gcagcaagct
     5101 ggcgggcgat ttgctggaga tcttcaccct gcaactccct gcgccagggg gcaatggcgc
     5161 caataccgcg tgcggcgcag gcatcaataa tggcgggtag cggcgtttta tagcccagtg
     5221 tcgcggtatt gatactgaat aaatccgggc gttgtaacag atctctcatg attcagcctc
     5281 agaagcgcgg catacttaag ccgccgtcga tggcgaccgc ctgaccacag gtgtaaatca
     5341 gtttaccttc cgccatcgcg cggacagtgg aagcaatatc agccggataa ccccaacgtc
     5401 cccacggcac cagacctttg gcaatcagtt cgtcgtaata ggccgttgcc ggaatagtca
     5461 tatcggtttt gatgagacca ggacgcactt catatacgcc aatttgttca ttacaaaggc
     5521 gagcagcaaa tagacgagca gcggcagaga cggcagtttt ggcaatggta tattcgccgc
     5581 gattcatcgc cagcatgatg gcgttgatgg aactgacgaa aataatcgag cggtgcggca
     5641 gttctgcttc cggtttcggc tgtgccagta agcgcttact gaatgcctga gcgaggaaga
     5701 acggtgcgcg ggtgttaatg gcaatatttt ggtcgaagct gtctggttcg aggtcgagaa
     5761 gatcaccgcg ttttttcact gaaatgcctg cgttgttgag caggcagtcc agacgtcccc
     5821 actggttttg cgctgcgtcg agcatctctt catgcaggga gagatcgccc acatcagcag
     5881 ggaagcaaat cacttccacg ccttccgcca gacattcttg ttgtgtggca tgtaattttt
     5941 ctacgctatc cgcatccgga cgatcattaa tcagcagatt aaatccgcca cgcgcaagct
     6001 ccagcgcaca gccctttcca ataccacgag cggctccggt aatcacggca acaggacgag
     6061 tggatttcat acaatagctc tctttttttg aattttgttt ccttagtgat gattagcttg
     6121 cgactggagg gtaaaaagat caaccatcta gttggttgat atgcgatggc gatcacagga
     6181 catttgataa cctgatgaaa gtacaagttt ttgccctttt ttctgaagct gaaatcaaat
     6241 caggttatga tagtcaccac gaagaggtcg tggggaagcg ttatgtatct tgataccagc
     6301 aacgtacaga gcctgaaaga aaagctgctg ttatgcgcgg ttaatgaatt tgccgaatat
     6361 ggttacgaag gggccagagt cgataatatc gtcaaggccg cggggtgcag taaacagacg
     6421 gtttatcacc actttggtaa taaagaaaat ttatttatcg aagtactgga atatacctgg
     6481 aacgatattc gacaaaaaga aaaggccctt gatttctcag atcttccgcc acagaaagcc
     6541 attgagaaaa taatcgattt tacatgggat tattatattt ctaatccctg gtttttaaaa
     6601 attgtgcata gtgaaaatca aagtaagggt gtgcattatg caaagtcaca gcgtctttta
     6661 gaaattaacc atgcgcactt gcagttaatg gaatccttat tggatgaagg gaagaaaaac
     6721 aatattttta agccagatat cgacccattg caggtgaata ttaatattgc tgcgcttggg
     6781 ggatattatt tgatcaacca gcatacgctt ggtctggttt atcacatcag tatggtttca
     6841 ccccaggcac tggaagccag acgtaaggtc atcaaagaga caatccttag ctggcttttg
     6901 gttgaccctt catctaccgc gcgcgaataa aactgccatc cggctggcgg gtaaacagga
     6961 cctgttgatt attccccgta tcaatggtta agcccgtcac cacgccgctg gcgttctggc
     7021 ggattttcac catctgcccg ttttgcaaat tactcagtgg cttatccgcg ccttctacct
     7081 gcgccatggc atagacatcg gtagcgggca gtccgtggtc gcggaacagc tgcgccatcg
     7141 ttttaccggg ttcgacacga taggagcgcc attgattatc aattccgcta tctggctgga
     7201 atggttgcgt atgggtggtt tgcggctgct cttctggctg gccttcctgt attggttctg
     7261 gagcgaccgg agccacctga tcaggatcat tttgtggcgt gactaattgc gcccgcagct
     7321 gttcttctgt cggtggttgt gattgcgact gaatatctaa ctgcgcttca cgcgtcacca
     7381 ccggcgcgtt gggcgtatca tcagatggaa gcagaaatcc gactaccaat acaatggcgg
     7441 caataatgat cccccgacga tgcatcggcg gcagcgggtc catgaagcga aaattgtccg
     7501 gcgcgtgcca gactttctcc agggttggtt ttagttcaaa gcgcccgggc atggtttcct
     7561 cctgctccgc gtcttgttcc tcaatcatag cctatgaata agctaacgct atgatgtccg
     7621 tggtaaaccc gcctttatta tattcatacg ggatattgct attgtttctt tttccccggg
     7681 atttgtcatc attcccgcga caaagtttta cccaaagaag tgtggctgat atgctgcccg
     7741 ctactttata ccctaagaaa ggaaatacga tgaccacccc aacttttgac accatcgaag
     7801 cgcaagcaag ctacggcatt ggtttgcagg tagggcaaca actgagtgaa tctggcctgg
     7861 aagggctgct gccagaagca ctggttgcag gcattgccga tgcgctggaa ggcaaacatc
     7921 cggctgttcc ggttgatgtg gtgcaccgcg cgctgcgtga aatccacgaa cgcgccgatg
     7981 ccgttcgtcg tcagcgtttc caggcgatgg ctgctgaagg tgtgaaatac ctggaagaaa
     8041 acgccaaaaa agaaggtgta aatagcaccg aatctggcct gcaattccgc gtgatcaacc
     8101 agggcgaagg cgcaattccg gcacgtaccg accgcgttcg tgttcattac accggaaaac
     8161 tgatcgacgg caccgtgttt gacagctccg ttgctcgcgg cgagccagct gaattcccgg
     8221 ttaacggcgt gatccctggc tggattgaag cactgactct gatgccggta ggttctaaat
     8281 gggaactgac tatcccgcag gaactggcat atggcgagcg tggcgcaggc gcgtccatcc
     8341 ctccgttcag caccctggtg tttgaagtcg aactgctgga aatcctctaa gcagcgcatt
     8401 ctgttcccct cgaaccagag gggagcagac attcagcaat aaacccttca gtttgccaaa
     8461 cggcgctatt ttgtgttgca aagaccccgt aagcgtgtat ttttgtgagc tgtttcgcgt
     8521 tatcaccgtg atatgacact cactttaaac ataaaattaa cattagatct aaatcttagt
     8581 attcataccg cgtattgtta cctaatatcg atgagtcccg atacagattc gtcgtatcat
     8641 agactgacta aaggccgtag agcctgaaca acacagacag gtacaggaag aaaaaaacat
     8701 ggtagatcag gtaaaagtcg ttgccgatga tcaggctccg gctgaacagt cgctacggcg
     8761 caatctcaca aaccgacata ttcagcttat tgccattggc ggtgccattg gtacagggtt
     8821 gtttatgggg tccggcaaaa cgattagcct tgccgggccg tcgatcattt tcgtttatat
     8881 gatcatcggt tttatgctct ttttcgtgat gcgggcaatg ggggaattgc tgctttcgaa
     8941 tctggaatac aaatctttta gtgacttcgc ttccgattta ctcgggccgt gggcaggata
     9001 tttcaccggc tggacctact ggttctgctg ggttgtaacc ggtatggcag acgtggtggc
     9061 gatcacggct tatgctcagt tctggttccc cgatctctcc gactgggtcg cctcgctggc
     9121 ggtgatagtg ctgctgctga cgcttaacct cgccaccgtg aaaatgttcg gtgagatgga
     9181 gttctggttt gcgatgatca aaatcgtcgc cattgtgtcg ctgatagtcg tcggcctggt
     9241 catggtggcg atgcactttc agtcaccgac cggtgtggaa gcgtcattcg cgcatttgtg
     9301 gaatgatggc ggctggttcc cgaaaggttt aagtggcttc tttgccggat tccagatagc
     9361 ggttttcgct ttcgtgggga ttgagctggt aggtacaaca gctgcggaaa ccaaagatcc
     9421 agagaaatca ctgccacgcg cgattaactc cattccgatc cgtatcatta tgttctacgt
     9481 cttcgcgctg attgtgatta tgtccgtgac gccgtggagt tcggtagtcc cggagaaaag
     9541 cccgttcgtt gaactgtttg tgttggtagg tttgcctgcg gctgccagcg tgatcaactt
     9601 tgtggtgctg acctctgcgg cgtcttccgc taacagcggt gtcttctcta ccagccgtat
     9661 gctgtttggt ctggcccagg aaggtgtggc accgaaagcg ttcgctaaac tctctaagcg
     9721 cgcagtaccc gcgaaagggc tgaccttctc ttgtatctgt ctgctcggcg gcgtggtgat
     9781 gttgtatgtg aatcccagcg tgattggcgc gttcacgatg attacaaccg tttccgcgat
     9841 tctgtttatg tttgtctgga cgattatcct ttgctcgtac ctggtgtatc gcaaacagcg
     9901 tcctcatctg catgagaagt cgatctacaa gatgccactc ggcaagctga tgtgctgggt
     9961 atgtatggcg ttctttgtgt ttgttctggt gttgttgaca ctggaagatg acacccgcca
    10021 ggcgctgctg gttaccccgc tgtggtttat cgcgctgggg ctgggctggc tgtttattgg
    10081 taagaagcgg gctgctgaac tgcggaaata atcgcattat catgctggat ggcgcaatgc
    10141 catccagctt ttagatcact cacccgccag cgcgcgcggg aatagtacat tgttttccag
    10201 actgatgtgc tccatcaggt catcaatcag ttcattaatg ccgttataca tcgctttcca
    10261 ggtggtgcag gcttccggcg gcggtgtgac gttattggtg gtgtgtttga tcacttccag
    10321 cagttcgccc gcttcatcgt gctcgctttc cattacgctg attggcccca ttgcctggct
    10381 gcccatgcct tgtttaatca tcgggaagag aatttgctct tctttcatca tgtggctgga
    10441 aagctcttca tgcagcatag tcaggtattt tgtcagccct tttggcacac ttggtttgtc
    10501 ggcgtgaacg cgctcgactt tagtcgcttg caggatcagc tccggcagtt gctcgcggtg
    10561 gcgatcgtgg tagcgcacga tgatatggtc gatgatttct gccagcgggg cgctacgcca
    10621 gtctttctca atcggttgtt cagcaagctt tgccagttcg gcttcaatga cgtcaacatc
    10681 cagttcttta cgtgccgccg cgcgcgccag cgtctgctta ccgccacagc agtaatccat
    10741 atcatattta cgaaacagag ccgaagcgcg aggaatagag agcgccagtt cacctaaagg
    10801 ttggtcgcga taagccatag ctgatacctc attcttaata agataagatg tattttaaat
    10861 gcatctttat ggcaaatgct ataaccctta cgtagcagag gcttatttaa ctcactgcca
    10921 gcgtgtcaac tttctgagac agattcagtt aaaggttttt ctggttttgc tctgactgca
    10981 atgaccacgc caaccactaa cagcgcgata ccgcttagcg tcattagcgg cggcatttgc
    11041 tggcgtaaca aaaaggtgta cagcaagcct gccagcgttt cgaaaacaat cagcggcccg
    11101 agaatcactg tcggtaatcg ctggctggcg acgttccagc agagtgcgcc aacccatgag
    11161 caaagcacgg ctatcgcaac catcagacta ataaacacca gcggacgggg gccaaagggt
    11221 agggagaagc ccggcgtttg cgtattcagc cagtaacagg cgacgagata gccgatgaga
    11281 gaaaccggca gcgtgaccag cgcctgcgcc gtcgcccaca tcatcggatg tttgtcggga
    11341 ttctcccgca gccagcgggc gttgcgcagg gcataccatg cccagcagac cacggaaact
    11401 aacgccagca cgatgccaga ggtataacgt gcccagtcaa aatcggggag tccgtggttt
    11461 aactcagcaa tattcacaca cgccaggccg atgccaatac aaaccagtgc cggggcgagt
    11521 tttccccacg cgagtttgcc gtcgcgctgg ctataaagca gattggcaaa aacgggaatc
    11581 accaccggca gggtgccaat aatcatcgtg gaaacaggcg cgccagtacg ttgaatggca
    11641 ctggcaaggc agaagtaata gatgaggttg cccatcatgg tgagcatcaa ggcggtaagc
    11701 cagtcgcgac gcgccaactg acgcagacgc acgcgtccca gccaggcaat gggcagcgca
    11761 attaacccta acgccagata acgccccatc gactgcaaca tcgccgggta ttccggcacg
    11821 atcaacggcc cgacaaaaat aagcccccac atcaaccctg ctaacagggc gtacagcacg
    11881 ccgctaatca ttactggcat ccattggtct gtcagaagaa agttcaggaa gcagacatga
    11941 ttgctcccgg catcccgtca ttatcagaga ggatgcggga ggatgccact ttaattaact
    12001 agttattaac attaaaaaga tggcttacgc tttcggctaa cgttgtcgtt gggcggccaa
    12061 tcaatttgct aagcgttttg ctgtcatcaa acagaccgcc tttcgatgcg ccaacgtcag
    12121 aatccgccag catatccgcc agtccatcag gcagtccgac gcttttcagt gccgcagcga
    12181 aatcagcttc gctcaggttt tgataggtaa cctgtttgcc gctctgtttg gtgagttctg
    12241 ccgctaactg tgtcagcgtc caggcactat cgcccgccag ttcgtaaacc ttgccttcgt
    12301 gaccggcttc gctaatcacg cgtgccgcag ctaccgcata atcagcccgc gttgctgagg
    12361 caattttgcc atcgcctgcg gcaccgataa atacgccgtg ctccagtgct gccggggcgc
    12421 tggcgaggta gttttcggtg taccagccgt tgcgcagcag ggtgtaaacg atgccagaat
    12481 cagccagcat tttctccgtc tcgatgtgct catcggcgag gccgagcggg gaggaatcgg
    12541 catgtagcag gctggtataa gcgataaatt tcacgccggc cgcctttgcg gcattaataa
    12601 cattacgatg ctgcggggca cgttgaccca cttcgctgga agagatcagc agtagttttt
    12661 ccactccctg aagtgcagat gtcagtgcgg cttcatcgcc gtaatcagcc tgacgcacgg
    12721 taatgccttg tgctgtcagt gcctgtgctt ttgccggatt acgaacgata gccactattt
    12781 ggctggcagg aaccgttttc atcaaggatt caataacata gtgaccaagt tggccagtgg
    12841 caccagtaat agcgatcatg ggaagtctcc atcgttttgt cttgtgttta aatacgctaa
    12901 cacttaaact tacttttagt aagtacgtac aaaaaggtaa gtatgagatg agtcaggtta
    12961 gcctgtcgca acaactgaaa gagggaaatc tcttcgctga acagtgtccg tcgcgcgagg
    13021 ttttgaaaca cgtcaccagc cgttgggggg tgttgattct ggtggcgcta cgcgaaggta
    13081 ctcatcgctt tagcgacctg cggcgcaaaa tgggcggggt gagtgaaaag atgcttgcgc
    13141 agtcgttaca ggcattagaa caggatggtt ttcttaaccg tatcgcgtat ccggtggtgc
    13201 cgccgcatgt ggaatatagc ctcacgccgc tgggcgagca ggtgagcgaa aaggttgctg
    13261 cactggcgga ctggattgag ttgaatttgc ccgaggtgtt agcggtgcgg gatgaacgtg
    13321 cggcataact gccggatgcg ctgcgcttat ccggactacg gttcatggct tgtagggcgg
    13381 ataagatgcg tcatcatcgc atccgccaat aaatgccgga tgcggttgat gatttgtagg
    13441 ccgaaaaaga agtgttttac ttactcagat ccacctgata aatcgcaaac ccgatatcat
    13501 cggtcgcgac tttattcatc ggatactgcc ctttctcttt aataaacact gcggctttat
    13561 ctgacggaga ggtttcgaaa cggatatcca gtttcttatc gccagctatt ggtgctaaac
    13621 gccagttgtt atctgccgcc gggtgaattt cccccgcacg tttcgactca tcagcaatcc
    13681 acgctgccag caccgagcgg ttctcatccg gtgaagcaaa agcgatatgg ctgtcgcccg
    13741 taccggcaaa tttgccgccg taagcgcgat agttattggt ggcaacgagg aacatggcgt
    13801 tcggatcaat cggcttgccg ttaaaggtca ggttcttaat cctttccgca ttggcattaa
    13861 tcatctggca ctcgccgtca taacgggcgg gctgggtaac atcaatctga taattcacac
    13921 catcaataac atcaaagtta taggtgcgga aaccatccca gttgatgagt gactgtggtt
    13981 tcgtgctgtt aggatcaatc tggttaaact gtccggcgga gcactccagc cactctttca
    14041 cctctttacc gctggctttc accacaatca gcgtattggg gtagagataa agatcggcgg
    14101 cattacggaa ggtcaactgg cctttttcca cctccacata gcttgccggg tcatttttgc
    14161 gaccaccaac tttaaacggt gcggcagctg aaagcactgg cagttttgcc agatccggat
    14221 cgccctgaat gtaatgctcg acatacgctt tttgcgcgtt gttcaccacc tgcacggtcg
    14281 gatcgtcctg cacaagcgcc agatagctat acatattgtc ggcagattta ccgattggct
    14341 tgctgacgaa ctggcgtgtg gcatcgtgat cggctttgag tgtttctacc agcttgctgt
    14401 cttccgccgc gagggatttt ttattcgcga tgtcgtaaat cggccgtgct tccgctttcg
    14461 cctgcgtcac ctgccattta ccgctgtcat tactgagttg taagtcgacc acaccaagat
    14521 gatcgcccca catgcctggc attaccgccg gaacaccatt cagcgtgcct ttcgcgatat
    14581 cagccccttc gatatcagca aaatctttac ctgggaaaac ggcgtgagca tggccaaaca
    14641 taatggcgtt aacgcccgga atttcactga ggtaataaac tgagttttcc gccatcactt
    14701 tatacggatc ggcagacagc ccggaatgcg ccagaacgac aacgacatcg gcacctttct
    14761 cgcgcatttc aggcacgtat ttgcgcacgg tttcggtaat atcattcacc gtcactttcc
    14821 cggataaatt agctttatcc cagcccatga tttgtggcgg gacgacgcca atataaccaa
    14881 tcttcagcgt ctgttttttt ccgtctttat cgaccacttc ggtgtcttta attaaatacg
    14941 gtgtaaacat tggctgtttg gttctggcgt caatgacgtt ggcatttaca taagggaatt
    15001 tcgctcccgc cagcgcattt ttcagataat ccagaccgta gttaaactcg tggttgccaa
    15061 gcgtgccgac ggtatagtcc agcgtattta atgccttata gaccgggtga atatcacctg
    15121 cttttaaacc tttcgccgac atgtaatctg caagcggact cccctgaatc aaatcgccgt
    15181 tatcgaccag tacgctgttt ttcacttcat tgcgggcatc gttaatcagg cttgcggtac
    15241 gtaccagtcc gaatttttcc gtggcggtgt ctttgtaata atcgaaatcc atcatgttgc
    15301 tatgcagatc agtggtttcc atgatacgca gatcgaccgt cgctgcattc acactggcgg
    15361 caatcagcgt ggccaggagc gttgcgctaa acttaatcat cagggacatc cttttatcat
    15421 cgggaatacg aaagaaaagg gagaataaac gtcttactta tagaacagtg aagaatgcca
    15481 caattttacg ctttgaaaat gatgacacta tcacagttgg cgcattcatt aacgataggg
    15541 tataagtaaa acaataagtt aacaccgctc acagagacga ggtggagaaa tgttagatca
    15601 agtatgccag cttgcacgga atgcaggcga tgccattatg caggtctacg acgggacgaa
    15661 accgatggac gtcgtcagca aagcggacaa ttctccggta acggcagcgg atattgccgc
    15721 acacaccgtt atcatggacg gtttacgtac gctgacaccg gatattccgg tgctttctga
    15781 agaagatcct cccggttggg aagtccgcca gcactggcag cgttactggc tggtagaccc
    15841 actggatggt actaaagagt ttattaaacg taatggcgaa ttcaccgtta acattgcgct
    15901 catcgaccac ggcaaaccga ttttaggtgt ggtgtatgcg ccggtaatga acgtaatgta
    15961 cagcgcggca gaaggcaaag cgtggaaaga agagtgcggc gtgcgcaagc agattcaggt
    16021 ccgtgatgcg cgcccgccac tggtcgttat tagccgatcc catgcagatg cagagctgaa
    16081 agagtatctg caacagcttg gcgaacatca gaccacgtcc atcggctctt cgctgaaatt
    16141 ctgcctggtg gcggaagggc aggcgcagct gtacccgcgc ttcggaccaa cgaatatttg
    16201 ggacaccgcc gctggtcatg ccgtggctgt agctgccgga gcgcacgttc acgactggca
    16261 gggtaaaccg ctggattaca ctccgcgcga gtcgttcctg aatccggggt tcagagtatc
    16321 tatttattaa ttaatttatg cagcaggtcc atcacttgct gcacctcttc ctgagtaagc
    16381 gccccgtctt tggcccattg cacgcgaccg tctttatcca gcaccactac agcggaactt
    16441 tcttcatcca gctgccaggc accgcgcgcg acgccattgc tatcgacaat aaactgcgac
    16501 cagggataaa gctttttatt actctccaga ctgctgcgca caaacatgcc ggaacccgga
    16561 attgcgtcgt cggtgttaac aatggtggtg gtctggtaac gatcgtgcgg taactttgct
    16621 gatttaatcg cttcaatcag cgtcgcgttt ttctcttttg cagaggtgcg accagcaata
    16681 tgttgcagta ctcgcacttt tcccactagc tgcgcgctgt tccaggtttt gtagctaaac
    16741 tgatctttat caagcaccaa ctcgccacga tcggtaatgc caatcggcgg cactcgctga
    16801 ccggtttcga actgatgtgc ggaagccatc atcggcaaca gcaggcaggt gagtgccaga
    16861 atcttgcgta gggtcatggt gtttccttct tatgatatgc aggtgatccg accacttggg
    16921 tctgagtttt aatcatatgt gctatttatc gaatttcccg caagtgtgat gccagtttgc
    16981 ggtcaagcgc acaaatcata tgaaaaatga atgcttatac tgaagacggc gcttcggtaa
    17041 aaagataatt ctgaataatt gtaaccttta ggtaaaaaaa gttatacgcg gtggaaacat
    17101 tgcccggata gtctatagtc actaagcatt aaaatttgcg cctcataata gttgggccga
    17161 ttgtggcacc gcacaggcgt aatactcagc aggagataac aatgaaaatt ttccaacggt
    17221 acaacccact tcaggtggcg aagtacgtaa agatcctgtt ccgtggacgg ttatacatca
    17281 aggacgttgg cgcttttgaa tttgataagg gtaagattct tatcccaaaa gtgaaggata
    17341 agctgcattt gtcagtgatg tccgaagtta accgtcaggt tatgcgtctg caaacagaga
    17401 tggcttaacc aaagtgctat gcagtaataa gacggctcct gattcaggag ccgttgatgt
    17461 ttctgggggt tacgcgacgc tttcttcttt atctttagcg tcaggcaatt ttggcgaaag
    17521 ggcggtggcc ttgctgtcga ttcgggtcac cagcagctgg tcgatgcggt agttatcgat
    17581 atccaccact tcaaatttgt agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt
    17641 acgcagcata aacatcataa agccgccgat ggtttcgtag ttgcccgact gcgggaactc
    17701 gtcaatatcc agcacgcgca tgacgtcgtc aataggcgtg ccgccgtcta tcagccatga
    17761 gttctcatca cgggcgacaa tttgctcttc cagcccctga ccgaccagat cgcccatcag
    17821 cgtggtcatc acgtcgttga gggtgatgat ccccactacc agcgcgtact cgttcatgat
    17881 caccgcgaag tcttcacctg cggttttaaa actttccaac gcctctgaaa gggttaacgt
    17941 atccggcaca atcagcgtat tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt
    18001 agccagcacg cggttcagca ggtctttaga atcgacatag ccgatgatgt ggtcaatatc
    18061 ttcattacag acgagaaact tagagtgcgg atgttccgcc accttattct tcagactttg
    18121 ctcatcttcg tggagatcaa accaaatcac gttttcacgc ggtgtcattg aagacggaac
    18181 ggtacgggat tccagctcaa agacgttttc aatcagctcg tgttcctgtt tacgtaacac
    18241 gcccgccagc gcaccggctt ccactaccgc gtagatgtca tcagaagtga tatcatcttt
    18301 acgtaccatt ggcagtttga aaatacggaa gattatgttc gccagtccgt tgaagaacca
    18361 caccagcggg gtgcaaacgt acaggcagaa gcgcatcggg ttgatgatac gcaaagccac
    18421 agcttctggc gcaatcatac cgatgcgttt cggggttaaa tccgcaaaca ggataaacat
    18481 gccagtcact aacgagaaag agagaataaa gctcagttgc tcagagagtt ctgccgacat
    18541 atagcgggag aacaggctgt gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc
    18601 gagaatcgcc actgcgttca gaccgatctg gaccacggta aagaacatgc cgggattttc
    18661 ctgcatattc agaacgcgtt gggcatttat attgccttca tcagccagca gtttaagttt
    18721 gattttgcgt gaggcggcaa gcgagatctc ggacatcgag aagaacgcac ttacagcgat
    18781 caaacagagt atgactaaaa tactgtttaa catatcttat ccgacctttc aggccagatc
    18841 ctcggaaggg gaagttgatt aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa
    18901 gtgaaccggc attttcagtg gctagtatag cgtaaggtac tgtaaagccg ccagagggtt
    18961 aaaattcaga cagctgaaaa atgaaaaact gcctgatgcg ctatgccgaa caggcctacg
    19021 cagtccccgc aatatattga atttggacga ttttgtaggc ctggcaaggc gtttacgccg
    19081 tatccggcat aaacaaaatg cactttatca acaatctaca tttgtacccg taacgctatg
    19141 cttccggcgg cagacagacg ccaattccgc caattccaca gtaaccatac gggtttttat
    19201 gcagatattg ctggtggtca tcttcggcat aataaaacgg tgtggcgtta gcgatttccg
    19261 tggtgatgcg acgatcgtca tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg
    19321 cgcgagcggc ggcatcctgt tctggggtca gcggataaat cgctgaacga tactgcgtgc
    19381 cgtggtcatt gccctgacgc attccctggg cgggatcgtg attctcccaa aatacttgta
    19441 gcaactgctc atagctgatg acggatggat cgtaaacaat gcgcaccgct tcggcatggc
    19501 cagtatcgcc ggagcacact tcccgataag tcggatttgg cgtatagccg ccggtatagc
    19561 ctgcggcggt gctgtaaacg ccgggtaact gccagaacag acgctccaca ccccagaaac
    19621 aacccatcgc aaaaatggca atctccattc cgtcaggtac attggtcatt gagtgaccgt
    19681 tgaccgcatg cagtgtggct acgggcatcg gggtgttacg tccaggcagg gcatcggcgg
    19741 gggaaaccag atgcttttta tcaaataaac tcatggtgtc gctctcccga aaatcggtca
    19801 ttggggttaa ggttgtaaca agaggcgtat ttgcacacaa taaccaccgt gaataggtct
    19861 aaagtaaaac ataagaaata ttttgggttt agtctgcttt ttaatccata ttactggatt
    19921 tttgttaagc cgtttaacgg cgttccaggg gcaggaaaaa aggatattca ggagaaaatg
    19981 tgcgctatat ccgacagtta tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg
    20041 ccgcgaacgt ccgtctacag gtcgaggggt tatcgggaca gctggaaaag aacgttcgcg
    20101 cgcagctttc tacgattgaa agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg
    20161 tcgatgatgc catccgcgaa ggtctgaaag cgctgggcta ttaccagccg accattgaat
    20221 ttgatctccg tccaccgcca aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag
    20281 gcgtgccggt gttaattggc ggcaccgatg tggtattgcg cggcggcgcg cggaccgata
    20341 aagactattt gaaattgctc gatactcgcc cggctattgg tacggtactg aaccagggcg
    20401 attatgaaaa tttcaaaaag tccttaacca gcattgcgtt gcgtaaaggt tatttcgata
    20461 gcgaatttac caaagcgcag ctgggcattg cgctcggcct gcataaagcc ttctgggata
    20521 ttgattataa cagtggcgaa cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa
    20581 tccgcgatga atacctgcaa aatctggtgc cgtttaaaga gggcgatgag tacgaatcga
    20641 aagatctggc agaactgaac cgtcgacttt ctgctaccgg ctggtttaac tcggtggtgg
    20701 tggctccaca atttgataaa gcgcgcgaaa cgaaagtatt accattgacg ggcgtggttt
    20761 cgccgcgaac agaaaacacc atcgaaaccg gcgtcggtta ctctacggac gtgggaccgc
    20821 gcgtgaaagc gacgtggaaa aagccgtgga tgaactctta cggtcacagt ttgaccacca
    20881 gtaccagtat ttccgcgccg gaacagatcc tcgacttcag ctataaaatg ccgctgctga
    20941 agaatccgct ggaacaatat tatttggtgc agggcggttt taagcgcact gacctgaacg
    21001 ataccgagtc tgactccact acgctggtgg cttctcgcta ctgggatctc tccagcggct
    21061 ggcagcgtgc cattaacctg cgctggagtt tcgaccactt tacccagggt gaaattacca
    21121 acaccacgat gctgttttat cctggggtga tgattagccg cacgcgttct cgtggtggcc
    21181 tgatgccaac ctggggcgac tcgcaacgct actctatcga ctactccaac acggcctggg
    21241 gctcggatgt tgatttctcc gttttccagg cgcagaacgt ctggatccgc acactgtacg
    21301 atcgccatcg ttttgtgaca cgcggcacgc tgggctggat tgaaacgggt gatttcgaca
    21361 aagtaccgcc ggatctgcgt ttcttcgccg ggggcgatcg cagtattcgc ggctacaaat
    21421 acaaatctat cgccccgaaa tacgccaacg gtgacctgaa aggggcctcg aagctgataa
    21481 ccggatcgct ggaataccag tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg
    21541 atagcggcga agcggtaagc gatattcgcc gcagcgactt taaaaccggt accggggtcg
    21601 gcgtgcgctg ggaatcgccg gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg
    21661 ataaagacga acacgggtta cagttttaca tcggtctggg gccagaatta tgagtttatg
    21721 gaaaaaaatc agcctcggcg tggttatcgt tatcttactg ttgctgggat cggtggcgtt
    21781 tctggtgggc accaccagcg gcctgcatct ggtatttaaa gcggcggatc gctgggtgcc
    21841 aggactggat attggcaagg tcaccggcgg ctggcgtgat ctcaccttgt ctgacgttcg
    21901 ttatgagcag ccaggcgtgg cggtaaaagc gggcaatctg catctggcgg tcgggcttga
    21961 gtgcctgtgg aacagtagcg tttgtattaa tgatctggcg ctgaaagaca ttcaggtcaa
    22021 catcgacagt aaaaaaatgc ctccttctga acaggttgaa gaagaggaag atagcggtcc
    22081 gttggatctc tccatgccgt atcccattac cctgacacgg gtggcgctgg acaacgtcaa
    22141 catcaagatt gatgacacca cggtatcggt gatggacttc acctccggcc tgaactggca
    22201 ggagaaaacc ctgaccctga aaccgacatc gctgaaaggc ctgttgattg ctttgccgaa
    22261 agtggcggaa gcggcgcagg aagaagtggt cgaaccgaaa attgaaaatc cacagccgga
    22321 ggaaaagccg ctcggcgaaa cgctgaaaga tctcttttct cgcccggtat tgccggaaat
    22381 gaccgacgtg catctgccgc tcaacctgaa cattgaagag tttaagggcg agcaactgcg
    22441 cgtgacgggc gacacggaca tcaccgtgcg caccatgctg ctgaaagtga gcagtatcga
    22501 cggcaatacc aaactggacg cgctggatat cgactccaac caggggatcg tcaacgccag
    22561 cggcacggcg cagctgtcag acaactggcc ggtggatatc accctcaaca gcacactgaa
    22621 cgtggagccg ttgaaaggtg aaaaggtgaa gctgaaagtg ggcggtgcgc tgcgcgaaca
    22681 gctggagata ggcgttaacc tttccggtcc ggtggatatg gatttacgcg cccagacgcg
    22741 actggcggaa gccggattgc cgctcaacgt ggaagtgaac agcaaacagc tttactggcc
    22801 gttcactggt gagaagcagt atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat
    22861 gaccgattat gcgatctcta tgcgcacggc tgttaagggc caggagatcc cgccagccac
    22921 cattaccctt gatgccaaag gtaatgaaca gcaggtcaat ctcgacaaac ttaccgtcgc
    22981 ggcgctggaa gggaaaacgg aacttaaggc gttgctcgac tggcagcagg ccattagttg
    23041 gcgcggtgag ctaacgctta acggtattaa caccgccaaa gagatcccgg agtggccgtc
    23101 gaaactcaat ggcttgatta aaacccgcgg tagcctgtac ggcggcacct ggcagatgga
    23161 ggtgccagaa ctgaagctga ccggtaacgt caaacagaac aaagtgaacg ttgacggcac
    23221 gctgaaaggc aacagttata tgcagtggat gatcccaggg ctgcatctgg aactggggcc
    23281 aaacagtgcc gaagtgaaag gcgagctggg ggtaaaagat ctcaatcttg atgccaccat
    23341 caacgcgccg gggctggata acgcgctgcc ggggcttggc ggtacagcga aagggttggt
    23401 gaaagtacgc ggcacggtgg aagcgccaca actactggca gatatcaccg cgcgcggcct
    23461 gcgctggcag gaactttccg tggcgcaggt tcgcgtggaa ggcgatatca aatccaccga
    23521 ccagattgcg ggtaaactcg acgtacgcgt tgagcaaatt tcgcagccgg atgtaaatat
    23581 caacctcgtc accctgaatg ccaaaggcag cgaaaagcag cacgagctac agttgcggat
    23641 tcagggcgag cctgtctccg ggcagcttaa tctggcagga agttttgatc gcaaagaaga
    23701 acgctggaag ggaactctta gcaatacccg cttccagacg ccggtcggcc cgtggtcgct
    23761 gacccgcgat attgcgctgg attaccgcaa taaggagcaa aaaatcagca tcgggccaca
    23821 ctgctggctt aacccgaatg cggaactgtg cgtgccgcaa actatcgatg cgggtgccga
    23881 agggcgtgcg gtggtgaatc tcaaccgctt cgacctggcc atgctgaaac catttatgcc
    23941 agaaaccact caggccagcg gtatcttcac gggtaaagcg gacgttgcct gggacaccac
    24001 gaaagagggg ctgccgcagg gcagtatcac cctttcgggg cgtaatgtgc aggtgacgca
    24061 aaccgtcaac gatgcggcgc tgcccgtggc gtttcagacg ctgaatctga cggcggaatt
    24121 gcgtaacaac cgtgccgaat tgggctggac catccgcctg accaataacg gccagtttga
    24181 tggacaggtg caggtgaccg atccgcaagg ccgccgtaat cttggtggca acgtcaatat
    24241 ccgtaacttc aaccttgcga tgataaaccc catctttacc cgtggggaaa aagcagcggg
    24301 gatggtgagt gccaacttgc gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca
    24361 gcttcaggtt acgggtgtgg atatcgacgg caactttatg ccgtttgata tgcaaccgag
    24421 ccagcttgcg gtcaacttta acggtatgcg ctcgacgctt gccggtacag tacggaccca
    24481 gcagggtgaa atctacctga acggtgatgc cgactggagc cagattgaaa actggcgggc
    24541 gcgggtaacg gcgaaaggca gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga
    24601 tgtatcgcca gatgttgtat tcgaggctac accaaacctg tttaccctcg atggtcgcgt
    24661 ggatgtcccg tgggcgcgca tcgtggtgca tgagctgccg gaaagcgcgg tgggcgtctc
    24721 cagcgatgtg gtaatgctta acgataacct gcaaccggaa gaggcaaaaa cggcgtcgat
    24781 tccgattaac agcaacctga ttgtccacgt cggcaacaat gtgcgcattg acgcctttgg
    24841 cctgaaagcg cggctgacgg gcgatctcaa cgtcgttcag gacaaacaag ggctgggcct
    24901 gaacgggcag atcaacatcc ctgaagggcg cttccatgcc tatggtcagg atctgattgt
    24961 gcgtaagggt gagttactgt tctctggtcc gccggatcaa ccgtatctta atatcgaagc
    25021 tattcgtaac ccggatgcta cagaagacga cgtaatcgcc ggagttcgcg tcactggtct
    25081 ggcggacgaa ccgaaagcgg agatcttctc tgacccggcc atgtcgcaac aggctgcctt
    25141 gtcttatttg ctacgtggac aagggctgga gagcgatcag agcgacagtg cggcgatgac
    25201 ctcgatgctg attggtctgg gggttgcaca aagtggtcag attgtgggta aaatcggcga
    25261 gacgtttggc gtaagcaatt tagcgctcga cacccaggga gtaggcgact cctcccaggt
    25321 ggtggtcagc ggctatgtat tgccaggtct gcaagtgaaa tacggcgtgg gtatatttga
    25381 ctctatagca acactcacgt tacgttatcg cctgatgcct aagctatatc tggaagccgt
    25441 gtctggtgta gaccaggcac tggatttgct ctatcagttc gagttttagc aatgcgaata
    25501 tttgtctacg gcagtttacg ccacaaacaa ggcaacagtc actggatgac caatgcccag
    25561 ttactgggcg atttcagtat cgataactac cagttgtata gcctgggcca ctatccaggc
    25621 gcagttccgg ggaacggaac agtacacggt gaagtttatc gtattgataa cgccacgctg
    25681 gccgaacttg atgccttgcg caccaggggc ggtgaatacg cgcgccagtt gattcagacg
    25741 ccgtacggga gtgcatggat gtacgtttat caacgacccg tcgatggatt aaagctaatt
    25801 gaaagcggcg actggttaga cagggataag taataataaa agcacgccac cttcgggtgg
    25861 cgcgaaaact gatgtaatgc aatgaaaaat aaaggattat ttttcgcgtt gtccacattt
    25921 tgccatagcg atacatctta gcccgtaatg attcgcgggc taagcatata caaaatgtta
    25981 agcctgatac gggggtccta tgtttgatag ggaaatgata aacgaaaata aagaggcgtt
    26041 ttttctgtat gttaacggta gaggggagtt gtcggctcaa tgcgtgataa aggtggctga
    26101 aaaaggggat tatttacaag gatactcttt aactcgtcgc gcatttcgta cctttttgaa
    26161 agaccgtgtt attcagatgt tttctagcga ccaggagttg aataaggttg catctgaaac
    26221 tga
//
LOCUS       DAZGAP010000063        26176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGAP010000063 DAZGAP010000000
VERSION     DAZGAP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.063"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..303
                     /gene="ypdI"
                     /locus_tag="ACHLAM_002180"
     CDS             43..303
                     /gene="ypdI"
                     /locus_tag="ACHLAM_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO7853706.1"
                     /translation="MIFSLFLLVSIMACNVFAFSISGGRSETSYKETEKTSVMTTTHS
                     TKLQPSQAILFKLREDAPPLNLTEDITPPFSTKANYLIHPVR"
     gene            complement(380..622)
                     /gene="yfdY"
                     /locus_tag="ACHLAM_002181"
     CDS             complement(380..622)
                     /gene="yfdY"
                     /locus_tag="ACHLAM_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO7853707.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            975..1895
                     /gene="lpxP"
                     /locus_tag="ACHLAM_002182"
     CDS             975..1895
                     /gene="lpxP"
                     /locus_tag="ACHLAM_002182"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO7853708.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2251..2322
                     /gene="ypdK"
                     /locus_tag="ACHLAM_002183"
     CDS             2251..2322
                     /gene="ypdK"
                     /locus_tag="ACHLAM_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO7853709.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2387..3625)
                     /gene="alaC"
                     /locus_tag="ACHLAM_002184"
     CDS             complement(2387..3625)
                     /gene="alaC"
                     /locus_tag="ACHLAM_002184"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO7853710.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4002..5699
                     /gene="ypdA"
                     /locus_tag="ACHLAM_002185"
     CDS             4002..5699
                     /gene="ypdA"
                     /locus_tag="ACHLAM_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO7853711.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5714..6448
                     /gene="ypdB"
                     /locus_tag="ACHLAM_002186"
     CDS             5714..6448
                     /gene="ypdB"
                     /locus_tag="ACHLAM_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO7853712.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6461..7318
                     /gene="ypdC"
                     /locus_tag="ACHLAM_002187"
     CDS             6461..7318
                     /gene="ypdC"
                     /locus_tag="ACHLAM_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7853713.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPVRQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7321..9816)
                     /gene="ptsP"
                     /locus_tag="ACHLAM_002188"
     CDS             complement(7321..9816)
                     /gene="ptsP"
                     /locus_tag="ACHLAM_002188"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7853714.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDGCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLNLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQMLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9841..10878)
                     /gene="ypdE"
                     /locus_tag="ACHLAM_002189"
     CDS             complement(9841..10878)
                     /gene="ypdE"
                     /locus_tag="ACHLAM_002189"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7853715.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTIRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10878..11963)
                     /gene="ypdF"
                     /locus_tag="ACHLAM_002190"
     CDS             complement(10878..11963)
                     /gene="ypdF"
                     /locus_tag="ACHLAM_002190"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7853716.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVICR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIFADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQLKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGVEVLYAMPKTVLLTGEA"
     gene            complement(11978..13225)
                     /gene="fryC"
                     /locus_tag="ACHLAM_002191"
     CDS             complement(11978..13225)
                     /gene="fryC"
                     /locus_tag="ACHLAM_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO7853717.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13247..13573)
                     /gene="fryB"
                     /locus_tag="ACHLAM_002192"
     CDS             complement(13247..13573)
                     /gene="fryB"
                     /locus_tag="ACHLAM_002192"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO7853718.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13792..14757)
                     /gene="glk"
                     /locus_tag="ACHLAM_002193"
     CDS             complement(13792..14757)
                     /gene="glk"
                     /locus_tag="ACHLAM_002193"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO7853719.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKIEVQDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14961..16217
                     /gene="yfeO"
                     /locus_tag="ACHLAM_002194"
     CDS             14961..16217
                     /gene="yfeO"
                     /locus_tag="ACHLAM_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO7853720.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16332..16658
                     /gene="ypeC"
                     /locus_tag="ACHLAM_002195"
     CDS             16332..16658
                     /gene="ypeC"
                     /locus_tag="ACHLAM_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO7853721.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16799..18037)
                     /gene="mntH"
                     /locus_tag="ACHLAM_002196"
     CDS             complement(16799..18037)
                     /gene="mntH"
                     /locus_tag="ACHLAM_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO7853722.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18373..19575
                     /gene="nupC"
                     /locus_tag="ACHLAM_002197"
     CDS             18373..19575
                     /gene="nupC"
                     /locus_tag="ACHLAM_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO7853723.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19624..21813)
                     /gene="pdeA"
                     /locus_tag="ACHLAM_002198"
     CDS             complement(19624..21813)
                     /gene="pdeA"
                     /locus_tag="ACHLAM_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO7853724.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22021..22096)
                     /locus_tag="ACHLAM_002199"
     tRNA            complement(22021..22096)
                     /locus_tag="ACHLAM_002199"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22061..22063),aa:Ala,seq:ggc)
     gene            complement(22136..22211)
                     /locus_tag="ACHLAM_002200"
     tRNA            complement(22136..22211)
                     /locus_tag="ACHLAM_002200"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22176..22178),aa:Ala,seq:ggc)
     gene            22432..22791
                     /gene="yfeC"
                     /locus_tag="ACHLAM_002201"
     CDS             22432..22791
                     /gene="yfeC"
                     /locus_tag="ACHLAM_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7853725.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22793..23185
                     /gene="yfeD"
                     /locus_tag="ACHLAM_002202"
     CDS             22793..23185
                     /gene="yfeD"
                     /locus_tag="ACHLAM_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7853726.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(23236..24651)
                     /gene="gltX"
                     /locus_tag="ACHLAM_002203"
     CDS             complement(23236..24651)
                     /gene="gltX"
                     /locus_tag="ACHLAM_002203"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO7853727.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            24910..24985
                     /locus_tag="ACHLAM_002204"
     tRNA            24910..24985
                     /locus_tag="ACHLAM_002204"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24943..24945,aa:Val,seq:tac)
     gene            25030..25105
                     /locus_tag="ACHLAM_002205"
     tRNA            25030..25105
                     /locus_tag="ACHLAM_002205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25063..25065,aa:Val,seq:tac)
     gene            25110..25185
                     /locus_tag="ACHLAM_002206"
     tRNA            25110..25185
                     /locus_tag="ACHLAM_002206"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25143..25145,aa:Lys,seq:ttt)
     gene            complement(25296..>26176)
                     /gene="xapR"
                     /locus_tag="ACHLAM_002207"
     CDS             complement(25296..>26176)
                     /gene="xapR"
                     /locus_tag="ACHLAM_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO7853728.1"
                     /translation="RVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELE
                     NQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGVV
                     GTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTGF
                     TSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGF
                     SPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVYET
                     QQVTPALVKLLAALAQ"
BASE COUNT         6501 a         6948 c         6442 g         6285 t
ORIGIN      
        1 agtgaaccat tttcaggata actttctatg aaagtaaact taatgatttt tagcttattt
       61 ttattggtct ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtagaagt
      121 gagacgagct ataaagagac agaaaaaacg tcagtgatga cgacaactca ttctacaaaa
      181 cttcagccat cacaggcgat tttgtttaag ttgagagaag atgcgccacc attaaatctc
      241 acagaagaca ttacgccccc tttttcgaca aaggcgaatt atcttattca tcctgtgcga
      301 tagcgtctgt cacctggcag gtgaacaatc atgcctgatg ctgatgtgaa cacctgccca
      361 aatgaaccat cctgacccac tagcgcctgc cataccccac cataaagcga atcatcaagc
      421 cacaagccgg ggcaagaaac ataaatatcc ccgtcactag ttcattaccg ccagttaacc
      481 acatggtgaa gtagtaaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg
      541 ccgagaccac gttcagtacc tgaccgatgt atccgcttac gcagacaata cacagggcga
      601 gaaacatcca taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc
      661 agtcacttta atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa
      721 aacagcacgt taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat
      781 aatcagtagc tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc
      841 gtggttatgt cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc
      901 ccaaccaatt gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc
      961 tacagaaggt aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct
     1021 actggctcac atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg
     1081 ttctctgctt tctcggcacg cgtattggcg caatggcgcg accattcctg aaacggcgtg
     1141 aatctatcgc ccgtaaaaac ctggaacttt gtttcccaca gcattctgcg gaagaacgcg
     1201 agaagatgat tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg
     1261 cgtggttctg gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc
     1321 ttaaacgcgc acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc
     1381 tggaactggg cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac
     1441 ataataatca gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga
     1501 tgatcggcag aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat
     1561 ggtttgctcc cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg
     1621 tggaaaatgt cgccacaacc aatggtacgt atgtcctttc acgtctctct ggcgcagcca
     1681 tgttaaccgt aacgatggta agaaaagcag attacagcgg atatcgtttg tacatcaccc
     1741 cagagatgga aggctacccg gcagatgaaa atcaagccgc tgcctatatg aacaagatta
     1801 tcgaaaaaga gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa
     1861 cgcgcccggt gggagaatcg tcgctataca tttaacaaac tgctcttact ctttccctcc
     1921 tcaaatcagc cataataacc tcatggtttt tatggctttt ttcttaaggt tttttccttc
     1981 gctgaatctc taattattac cgtcagaaat taaactgtcg ccgatatgag acacctgtaa
     2041 gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag
     2101 cgccgtctgg agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacaggac
     2161 ttttaaggtc gttttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg
     2221 cgtaacagta attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt
     2281 atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga
     2341 aaaacaggag ccatctggct cctgtttgca tttctggcga tggtgtttat tccgcgtttt
     2401 cgtgaatatg tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaattc
     2461 cacgaatcgc ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtgt
     2521 cgccgtagtc gccaaagcca atccccggcg agacacagac cttcgcttcg ttcagcagct
     2581 tcttggcaaa ttccagcgag cccatggcag catacggttc cgggattttc gcccagacat
     2641 acatcgaagc cttcggcatt tcgaccatcc agcccgcttc atgcagccct tttaccagta
     2701 catcacggcg gcgtttgtac tgttcagcaa tgtcgcgcac gcactgttga tcgccctcca
     2761 gcgccgcaat cgctgccacc tgcaacggcg taaaggtgcc gtaatcgtga tagcttttaa
     2821 tacgtgccag cgcgctgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg
     2881 ccatgttgta gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacctggca
     2941 cctgcatgat tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat
     3001 ggaccaccag cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagct
     3061 ccacgcattg cgcggttgga ttcgacggga agccgaggat catcatcttc ggtttcggat
     3121 aactttcgcg aatggcgcgt tccagttcgt tgaagaaatc gacaccttcc accagcggca
     3181 ctgagcgtac ctgcgccccg gcaatcaccg cgccgtaaat atgaatcggg tagcttggat
     3241 taggcaccag tacagtgtca ccatgatcca gcgtcgccag catcagatgc gccagtccct
     3301 ctttcgaacc aatagtgacg atggcttctg attccgggtc gatctcaatg ccgtagcgat
     3361 cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgac gtggagtaac
     3421 catgcgtgtc cgggcgctgg gccaccgtac ataatttttc gacgatatgc ggcggagtcg
     3481 caccgtccgg gttacccata ctgaaatcaa taatatcttc gccgcgccga cgcgcagcca
     3541 ttttcagttc agcggtaatg ttaaaaacat agggcgggag acgatcaatg cgcgtaaagc
     3601 gacgttcagg gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc
     3661 cgagcgacgc tgccacgatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc
     3721 cagcgtttta gaacattttt attaatgacg ttaaatgagg aactttgctt taaaaacagg
     3781 aaatttatct gcgctaacaa gaaaattacc acccatttta ttaacataat aatttcaata
     3841 aatttactaa ccagaagtgc attatcaaga gatgcattgc taatacacca tttatctatc
     3901 aatccccttt taaaattgtg gtttttcctc aaaaacggcc tgctggtcat cagccttcag
     3961 gttacctatc atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca
     4021 tgctgctggc ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc
     4081 gtatccgtct gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gagttgctcg
     4141 ccgttaccgc cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcctgtag
     4201 aaggttcgct ggtgaacgta cgtattatcg cggtgatgtc aggcgggatt ctgtttggcc
     4261 cgtgggtggg catcattacc ggcgtgattg cgggtattca ccgttattta attgatatcg
     4321 gtggcgtgac ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcgggct
     4381 ggatcaacct gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt
     4441 tgtgtgaaac gttgaccatg attctggtga ttgtctgggc accaactacc gcattgggga
     4501 tcgatatcgt ctctaaaatc gggattccaa tgatcctcgg cagcgtctgt atcggcttta
     4561 ttgtgttgct ggtacaaagt gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca
     4621 agctggcgct ggatatcgcc aacaaaacgc tgccgctgtt tcgccatgtc aatagcgagt
     4681 cattacgcaa ggtctgcgaa attatccgcg atgacattca cgctgacgcg gtggcaatca
     4741 ccaataccga tcatgtgctg gcctacgttg gcgtgggtga acataactat cagaatggcg
     4801 atgacttcat tagcccgact acccgccagg cgatgaatta tggaaaaatc atcattaaaa
     4861 acaatgatga agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg
     4921 agaaaggcgt tgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagataacct
     4981 cgtcattaca ggaaatggcg gtcggtctgt cacaaatcat ctccacgcaa ctggaagttt
     5041 cgcgcgctga gcagctgcgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca
     5101 aaattaatcc ccatttcctg tttaacgctt tgaacgctat ttcatcgtca atccgtctga
     5161 atccggatac cgctcgccag ctgatcttta atctgtcgcg ttatctgcgc tataacattg
     5221 aattaaaaga cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata
     5281 ttgccattga gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag
     5341 aggtgaattg ctgcattccc agcctgctga tccagccgtt ggtggaaaac gccattgtcc
     5401 acggtatcca gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggta
     5461 atcgggtacg cattgcggtg cgggataccg ggcacggcat cgatccaaag gtgattgagc
     5521 gggtcgaagc gaacgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg
     5581 tgaagttatt gtatggcgag gggctgcata tccgccgcct ggaaccagga acggaaattg
     5641 cgttttacat tcctaaccaa cgcacccctg tcgcctcaca ggctacgtta ttgctttgag
     5701 ccggagtgat attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact
     5761 gagctggcta attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct
     5821 ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat
     5881 tccgtcgctg gatggtgtgt tactggcaca aaacatcagc cagttcgccc ataaaccgtt
     5941 tattgtgttt atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt
     6001 tgactacatt ctcaaaccgt atcaggagtc acgcattacc gggatgctgc aaaaactgga
     6061 agcagcctgg cagcaacagc agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa
     6121 tgacaccatt aatctggtga aagatgagcg aatcatcgtc acgccaatta acgatatcta
     6181 ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat cctacgtaat
     6241 gccgatgaac attaccgaat tttgcagcaa actgccgcca tcgcattttt tccgctgcca
     6301 tcgctcattt tgtgtcaatc tgaacaaaat ccgcgagatc gaaccgtggt ttaataacac
     6361 ctatattctg cggctgaaag atctggattt tgaagtgccg gtcagccgca gcaaagttaa
     6421 agaattccgc cagttaatgc atctttaatc gggaactttc atgaaagcac caggcttgcc
     6481 cgccgatcaa caatttttcg ccgatctgtt cagcggcctg gtgcttaacc cgcaactact
     6541 ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg gtgggcagtt tatgtattga
     6601 ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc aatctgctgg aagcaaagca
     6661 gcaacgtatg gttgaaggag aaatgctgtt tattccggcg cgcgcggcta atttaccgat
     6721 caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc ttgggttatc
     6781 gttttacgat agccgcacca cgtcgttgtt gcatccggtt cgccagatcc aacttcccag
     6841 cctgcaacgc ggcgaaggtg aagcaatgct tactgccctc acccatctta gccgttcgcc
     6901 gctggagcag aatatcattc agccgctggt gttaagtttg ctgcatcttt gccgtaacgt
     6961 ggtgaatatg ccgccgggca attcgcagcc gcgcggcgat tttctctatc acagcatttg
     7021 taactgggtt caggataatt atgcccagcc gctcacccgt gagagcgtgg cgcagttttt
     7081 taatatcacg ccaaatcatc tgtcaaaact gtttgctcag catggaacaa tgcgttttat
     7141 cgagtatgtt cgctgggtac gaatggcaaa agcgaggatg attttgcaaa aatatcatct
     7201 gtcgattcat gaagtggcgc agcgttgcgg gtttccggat agcgactatt tttgtcgcgt
     7261 tttccggcgt cagtttggtc tgacgccggg agagtacagc gcccgttttc agggctaacg
     7321 tcagaaggtt aattctgttt ccagcagcgt caggatgctt tgtgcatctt gcgcggcaaa
     7381 caacgactgg cggaagtttt tattcaccag tttacgcgcc agctgcgaga agactttcac
     7441 atgattcatc ccttcgttag cgcccagtgt cagcatgatc accagttcga cttcgcccat
     7501 ttctgactgc cagtcaaccg gtttcaccag tcgggcaatg ctgatactgg aatgacggat
     7561 ccactgagat ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg taacaatctc
     7621 ttcccgctgc cagacatcct cttccagctc gaacggatgt tcagtgcgcc cgttaacgcc
     7681 gaggttaccg cacaggaact ggatcgcctg ctctttattg ctaaaagcct gatcaacaaa
     7741 gatattctcc agcgccagca gagggcgaac gtcttcttcc ggcgtaaacg cggtgagtaa
     7801 cgcttcaatc tcctgcgcgc tgcggcattc acatgcctga cgcgccagtt cccgacacgc
     7861 ctcgctatcc agttgacgaa gctggctttt caccgccgga atgcgcgggc tactcatact
     7921 cagctcgtcc aggcccagcc caagcagtag cggcagataa cggctttcac cgcccagttc
     7981 accgcaaata cctacccatt taccccgctg atgcgcagtg gtaacaatct gctgcaacat
     8041 gcgcaggaac gatggcgtaa tcgggttata cagcggcgat acgcgcgggt tattacgatc
     8101 gaccgcatac agatactggg tcatatcgtt ggagccgata ctgaagaaat cgacctcatc
     8161 gcagaagtga tcgatgatgt agcacaccga cggcacttcc accatgatac ccagcgtaat
     8221 cgtctctgca tgacgcaggc catcgcgctt aagctcaacg atcgcttttt ggatctcgcc
     8281 cttcacccat aagatctgat cgaggctgtg aaccattggg atcatcaact gggcgttgcc
     8341 gaaactggcg gcgcgcaaaa tggcccgcag ttgagtgcgg aacaggccag caaattccgg
     8401 gtaaattcgt accgcacgat agccgaggaa cgggttctct tcctggggaa tgttgaggta
     8461 aggaatgctt ttatcgccgc cgatgtccat tgtgcggaag ataatcggct tgtcgcccgc
     8521 ggccagtagc acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg cactgtcgcg
     8581 atccatatag agcatttcgg tacgaaacag cccgacgcct tccgcgccgt tggcaaacgc
     8641 gccaggcgct tccagcgcgg tgccaatatt cgccgcgata tcaatgcgct tgttatcacg
     8701 ggaataggcc agctgcgcgg cggcctgcgc ctgctgctgc tgacgtttat ccgccagcat
     8761 ctgcgcaacc tgataataac cgctcaccgc gtcattcggg ttaatcgcca acacgccgca
     8821 ctgggcgtca agcaccgccg gttgcccggc atagcgcgca atggcatcca gcggcaggcc
     8881 gctcagtacc gggatcgccg aggcgcgggc cagaatcagg gtatgcgagg tgcgcccggt
     8941 tttctccaga atcatgcccg caagattttt taaattgagg ctcaaaaact ggctaggggt
     9001 taaatcctct gccaccagaa tggtcggttt ttccagcacc agattgttgc gcggttttag
     9061 ttccggccag gtgatatgca gtaactgttc gctgatatcg cgaatgtcgc taacccgctc
     9121 gcgcagataa tcactggcag aagcagaaag tttggcgcaa acctgctcca tattgctgat
     9181 gatcgccgcc cccagcccct gatgctgttc tgccatcagg tgacggatat tgcctgcaaa
     9241 ttcatcatcc tgaatcagcg acaaatgggc gctgaggata gttttgcttt cgccgtcacg
     9301 ctcacgcaat tgctggttca gttgctcggc aagcgttgcc aggctgtgct ccagccgggt
     9361 ggaatcttgc gcactggcgg ggatcgcccg gtaactgtcg aggctgtcgc tctgtaacag
     9421 ggtcagcgta cccacgccga cgccgcttgc cagcacattg ccgtacagta aatcaggatt
     9481 cagacggctt aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg ttggctggac
     9541 gctgtcgctg tcgataaagc gcacctggat gtactcttcc agcacgcgcc gcgcctgctc
     9601 ttcatcgctg ccgctaatgt tcaggctgca accgtcatta aatagggtgc cggtgccaat
     9661 cagcgccagc gagcttttgg catctgcctt tgcgttctgg cgatggttaa taaaagtgat
     9721 ttcgctttgc cactggctgc actgttcttt aagttcccac gccggacgag catgcagacc
     9781 gtttggcaaa ggacagagaa attgaatcgt taacataacc gctccgggtt aatcaggaga
     9841 tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta agacgttgaa taagggcaga
     9901 taaaagctgc tgcatctgca aaatgtcacg gcaatcggca atcgatgcgg cgcaatgtcc
     9961 gtggcgggtg gcaggcccca tcaccactgt gggtacgcca gtgccggtta agtgcaccgc
    10021 cccaccgtcc gtgccaccgt tgctgaacat atccgcctgc aacggcacgc caatttctgc
    10081 cgccacggtt tcaatccacg cggtaagttt tggcggcgca atcagcgatt tatcgcttaa
    10141 caccagcatc ggcccgttac caatctgacg atggttagcc gcgccataat caaagttttt
    10201 cgcccagcag gcggtatcaa gcacaatggc gacgtccggc gacaccgcgc gggtggcagt
    10261 ttgcccgccg cgtaatccca cctcttcgct ggaacttgcc accagccaca cctccgcagg
    10321 cagttcggcg tcgtgcagct cacgtagtaa cgtcaccagc agatagcaac cgaggcgatc
    10381 atcaaaggct ttccccatca cccgctggtg agggagaacc tgaaaagttg tatcaaaggt
    10441 gacgcgatcg cctggacgaa ttcccgcctg catcacttcg tcatacgagc gcgcaccaat
    10501 gtccacgcgt atggtgctga cgtcattccc ctgccggtcg ccgtcaagca ggcctggaat
    10561 tttgcactct tcacgggtag tgatacgcac cgactgaagc tggcgggcag ccatgcgtac
    10621 attgccaacc ggcaacacat cgatcgcccc ttcgcgggag atgctgcgca ccataaatcc
    10681 cacttcgtcc atatgcgcac agatcatcac cttcggacct gtcgactcat tgagtcggat
    10741 cagcaccgat cccagcccat caaatcgcac ttctttttgc aggcgatccg cttcttccag
    10801 caggatctgc cgtacttcct gttccgagga ggcgatcgca tctgcctcgc tcaacgcttt
    10861 taataacgat aaatccatta tgcctctccc gtgagcaaca ctgttttcgg catggcgtag
    10921 agcacttcta cgccttgcgg ggtgaccagc acaacatctt cgatgcgcac gcccccttgc
    10981 cctggcaaat aaatccccgg ctccacggtc agtaacatgc ctggctgtag cgtcgtggtg
    11041 tcccgcggtg aaaaacgcgg atcctcatgg acttcaatac cgatagcatg accggtgtta
    11101 tgaccgaaat agtcgccata accggcttct gtaatcaccc ggcgcgcggc gtcgtcaacc
    11161 tgctggcagc gtacgccggg gcgaatcgcg gagattgctg cgagctgtgc ctgcaggaca
    11221 atctgataga cgttaaacag cgggtgggat tcggcactta ccccttcgcc attcaccagc
    11281 aaggtgcgcg tcatatcaga gcagtagccc tgatacagcg caccgaaatc gagagtgaca
    11341 aactcgcccg ctgcaacaat cttgtcgctg gctttgccgt gcggcagcgc cccacgccag
    11401 ccgctggcga caatggtatc gaaagaggct ttttctgcgc cctgctggcg cataaaccac
    11461 tccagttcag cggctatctc gcgctcgctc atccccgcct gaataaagcg gcgaatatgc
    11521 tctgcaccgc gatcggcaat cccacaggcg agacggattt tctccacctc ctctggcgtt
    11581 ttgagttgcc gcagcacatc cggcgtggcg ctaaccagtt tcgcattgag ttcagactgc
    11641 cagcggtgcg cggtttccca actcacctgc tggccttcaa aaccgagcgt ttgcaactgc
    11701 tcatcggcaa agatttgcct gacgatggtg gcaagcgtgt gcatcgcgtc gagcaaatgc
    11761 agctggtaac cttgcgtgcg ggcttctaca tccgcgtaat agcgcgagtc caccagaatg
    11821 tgcgcacttt cacggcaaat caccacatag cctgatccgg tggagatccc cagatgcggc
    11881 tgtttgttct gccgtgagga gagaagcacc gcatccagtt gttgcgcctt aagccagtcg
    11941 cgcagcgaag cgagtaatgt catcaggatt gtccttctta caggctatcg attaacaatt
    12001 tgcctttacg gaacatcatc agacgcagga acaccaccat cagcgcagtt atgactgccc
    12061 ccagcgcgat ccccgccatg tagacgccga ggttagtgac cagcggccat gcccagatag
    12121 cggactccgg gaaccattgc actgcgccca gccagacagc ggcggttgag ccgacaatcg
    12181 cgccgaccat ataggacgga atggcggtga tggggctttc cagcgcaaac ggaatcgcac
    12241 cctcactgat ccccataaac gccaggaaca tggcggtttt accctgcgga taaagctgag
    12301 cactgaacag acgtttgccg gttaaacggc ggtcgataat ggtcgccaga cccagaccaa
    12361 tcggcggaat aacaatagcg atggaacgcg cggtaaccgg taatacgtgg tcagtggtaa
    12421 agctgaaggc aacgaaacca gctgctttgt tgatcgggcc accaaggtcg attgctgtcg
    12481 ccgcggcgat ccccatcgcg tacattagcg cacccttctc acctgcggca gtcagcacgg
    12541 tacggatacc gccgttgatc cagccaccaa agggggtgat gacgtagtac atcgccagca
    12601 tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt tttgaacgct aacaggaaat
    12661 ccggtaactg gattttctgg ttcatccact tcacaaggta accagcaacg atagaaataa
    12721 tcagggcacc aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc atcgtgctgg
    12781 gatcgaagtt cagcagttgc gtcggctggg tagacatcaa cccaccgata aaaccagccg
    12841 ggaacgccag tttgccaccg atagagttcg ccacaaatgc agcaaacatc ggaatggcaa
    12901 aaccaaacaa cacgccgccg aaggactgcg acaaccaggc aaatttcagt aaggagaggt
    12961 cgaaaccgga gaatttcccg ctattaagag catccatgat gccgatatca gcaggaattt
    13021 taagccagct ataagcaatc agctggctga aagcgaggat cacaccgccc ataattaagg
    13081 tcggcaccat gcgtgaaatc cctgacatca catgctgcgg gagttcaccc cagaagctgg
    13141 ttttagaggc ctgcggattc ttaactgccg ctgccgcacc ggatgcgcca ggcacaacgg
    13201 ttgcactgcg ttttttaatg gccataacaa ttatccctgt cgaatattac tgttgttcag
    13261 aggcaatcat ctcttcgatt tctttgatga tgcccgcagc gtttttaatt gcgtcctgca
    13321 aagtgatttc ataaacgtcg cgtgattcga aacgttcgtt atcttccggg gtaaccgcca
    13381 cggagtggat gatgatggtc gcttcggcga tatcctgcgc ggtcaggcga ttctggatgc
    13441 cgtccgcgcc ctgagtttca attttcactt catagcctgc ttccaccgcc gcttcttcca
    13501 gcgcctgggc tgccataaag gtgtgagcca gacccatcgg gcaggcacat aaagcaatca
    13561 gtttcttact catcgttaat tcctcgttca gtgatagttg acgagtgaaa ttatgaacgt
    13621 gcaaaacaaa tcgccgttac cagacaaata atgcatttac tggagaatta atcccgctgt
    13681 acggaagtgt gacgcaggtc ggccttgtga ggggacacaa aggaaaattg aattaactgt
    13741 atgatttaaa agattatcgg gagagttacc tcccgatata acaggaagga tttagagaat
    13801 gtgacctaag gtctggcgta aatgtgcacc ggaaccaaga aggcccggat tgtcatggac
    13861 gatgagatac accggaatat catggacata ttctttaaag cgccctttat cttcaaatgc
    13921 ggcacggaaa ccagaggctt tgaagaactc aaggaagcgc ggcacgatac cacccgcaat
    13981 aaacacgccg ccaaatgtcc cgagattgag cgccagattg ccgccaaaac ggcccataat
    14041 gacgcaaaac agcgacaacg cgcggcggca atcggtgcag ctgtcagcca gcgcgcgttc
    14101 ggtaatatct tttggcttga gattttctgg caggcggttg tcggctttaa caattgcgcg
    14161 atacaaattc accagcccag ggccagaaag cacgcgctct gccgaaacat gaccaatttc
    14221 tgcacgcagt atttcgagga taatgccctc ttcttcacta ttcggcgcaa aatcaacgtg
    14281 accgccttcg cctggcaagc ttacccaacg cttatcgaca tggaccagat gcgcaacccc
    14341 tagccccgtt ccggcaccgt aaaccgcaat aggcttacct tctaccggtt ctgcgccacc
    14401 aaactgaatc agatgctctt ttttcagcat cgggatcgcc atcgatacag cggtaaaatc
    14461 gttgataatt tccagatggc taaaaccgag attctttttc atttcggcaa ttgagaacgc
    14521 ccaggtatgg ttggtcattg ccacccagtc accggtaatt gggcaagcga tggcaataca
    14581 gccgtcttgc acctcgatct tatgttcttc aagataaacg cgaatgaccg cttcgaggct
    14641 ggggtaatca agccctgaat aggtcttagc ctgcgagatt tcaccactgg caatatcaca
    14701 cagagcaaga cgtgcgttgg tgccgcccac atcaccgact aatgcatact ttgtcattct
    14761 tcaactgctc cgctaaagtc aaaataattc tttctcacac tgtaaatacc tgggggcata
    14821 acaacaacgc cggaaaggca ggctccctgt aaatatcgat ctgggtcaca caattacttt
    14881 atcgtttcag caccaattgc agcgatgcct ttttgcaagc tgggcaaact aagtatctga
    14941 ccccgcataa ggaatagaac atgctccatc cgcgagccag aaccatgttg ttattatcgc
    15001 tccccgccgt ggcaattggg attgcgtcca gtcttattct gattgtggtg atgaaaatcg
    15061 cctcggtatt acagaatttg ctctggcaac gactgccggg aactctgggg atagcccagg
    15121 attcacctct ctggatcatc ggtgtattaa cgctaacggg tattgcagtg gggttggtta
    15181 tccgtttcag ccagggtcat gccggaccag accccgcctg tgaaccgctg atcggcgcac
    15241 cagttccgcc ctctgcgcta ccaggactta tcgtagcatt aattcttggt cttgctggtg
    15301 gcgtcagcct ggggccagaa catccgatca tgaccgtcaa tatcgccctt gcggttgcca
    15361 ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg gactatttta gcctctgccg
    15421 gaaccatcgg cgcgctgttt ggcacacctg ttgcggcggc gttgatattt tcgcaaacct
    15481 taaatggcag tagtgaagtt ccgctatggg atcgtctttt tgcgccgtta atggcggcag
    15541 cagctggtgc actaactacc ggattatttt tccatcctca tttttcactg cccattgcgc
    15601 attacggaca gatgcaaatg accgatattc tcagtggtgc aattgtcgcg gcgattgcca
    15661 tcgcagcagg gatggtcgcc gtatggtgct taccacgatt gcacgcgatg atgcatcaaa
    15721 tgaaaaatcc ggtgctcgtg ctgggtattg gcggatttat tctcggtatt ctgggggtta
    15781 tgggtggacc agtttcgctg tttaaagggc tggatgagat gcagcagatg gtggcaaatc
    15841 aggctttcag caccagcgat tactttttgc tggcggtgat taaacttgcc gccctggtcg
    15901 ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc ggcagtgttt gtcggcgtgg
    15961 cattagggtt gatgctgcat gagcacgtcc ccgccgttcc agcggcaata accgtttcct
    16021 gcgctattct cggcatcgtg ctggtggtaa cacgcgatgg ctggttaagt ctttttatgg
    16081 cggcagtcgt tgtacccaat accacattgc taccgctgct ctgtatcgtc atgcttccgg
    16141 catggctgtt attagcaggt aagccgatga tgatggtcaa tcgtccgaag caacagcccc
    16201 cccacgataa cgtttagcaa aaaatgctct cctttatgat ttaagagtta tggcgatgat
    16261 acggggcttt acctccccgt aatattgcgt taacaggccg ctgacggctt cgatcgtgaa
    16321 ggagaataac aatgttcagg tcactgtttc tggcggccgc cctgatggca tttaccccgc
    16381 ttgcggcaaa cgcaggtgaa atcaccctac tgccatcaat caaattacaa attggcgatc
    16441 gcgatcatta cggtaattac tgggacggtg gtcactggcg cgaccgtgac tactggcatc
    16501 gcaattatga atggcgtaaa aaccgctggt ggcgtcatga taatggctac caccgtggct
    16561 gggataagcg taaagcgtat gagcgtggct atcgtgaagg ctggcgcgat cgtgacgatc
    16621 atcgcggaaa aggccgcgga catggacacc gccattaaac acattcgcca atggagcaca
    16681 atgcctgatg cgacgctagt gcgtcttatc aggcctacaa accggctctt taacgcgtag
    16741 ggcggataag gcgtttacgc cgcatccgcc aatagtgcca gatgcgacgc tcattcaact
    16801 acaatcccag cgccgtcccc accaacaacc agatattcag cgccacgacc agcactacaa
    16861 tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag atcgcccatc aacttgctgt
    16921 cactggtgaa aatcagcagt ggaaccagcg ccagagcgat accaaaactt aacagcacct
    16981 gactcataac cagaatccgt gtcggatcta atcccatcag aatgacaata aatgacggca
    17041 acatggtaac ggtgcgacgc acccacagcg ggatatggaa gcgaatgaag ccctgcatca
    17101 ccacctgccc cgccagtgtc cccaccaccg ttgatgacag tccggcagca accaggctta
    17161 atccaaagac cgttgccgca gcatggctta acaacggttg cagcgtcagg taagcctcat
    17221 caagatcggc aacgccagta tgaccggaaa agtggaacgc cgccgcagcc gtagccatca
    17281 tcgccagatt gacaaaaccg gcaatagtca tagcgatagc cacatcccat ttggtggcag
    17341 aataacgttg ttgacgcgaa ccaccatgta aatgctgagt cagcgaagag tgtaaataaa
    17401 tcacgtgtgg cataatcgtc gcccccaaca cacctgccgc caggaagact gcctccgagg
    17461 tgggtaaact cgggattacc attcctttac ccagttgcgc caggttaggc tgggagaaaa
    17521 tcaactcgac aatgtaagcc gcagcaacaa acagcagtaa cccgccaatc actttctcca
    17581 gcggtttttg ccctcgacgt tgcagcatta aaatcaggaa agtcgcgatc cccgtcagca
    17641 ccgcgccctg caacagcgaa acacccagaa tgagtttaaa accgatcgcc gcaccaataa
    17701 attccgccag gtcggttgcc atcgcaatga tttctgcctg aacccaatag aaccacacta
    17761 cgggacgcgg atagtgatcg cgaatctgct ccgccaggtt tttaccggtg gcaatcccca
    17821 gtttggcaga cagtatctga atcagcatcg ccatcaggtt ggcccaaacg acaacccaca
    17881 gtagctgata gccgaagctg gcacccgcct gaatattggt cgcaaagtta ccaggatcga
    17941 tataaccaat cgccgcaatg aacgcaggtc ccattaatgc gagcctcatc ttgcgcgccg
    18001 cccgtccgct gctactctca acgcgatagt tagtcatctt gtgcctctaa aacatagcct
    18061 ttgctatgtt tcatgctatg tcaaacgaga atgattatca aattcattta agtggattgt
    18121 gatgatttct ttgatagacc agaattatga cgacgaaaag attgatgact tgaatgttgt
    18181 gattatgttt aatgttagca catttacata actttcagct tccatacaca acatagcaga
    18241 aatgtatgac agctcactat ttttgaagct tgtcacagga cgtcattata gtgtgtgtca
    18301 gatctcgttt tccttaacca tgttacatag aatgtgcacg gaaatttaac ctgcctcata
    18361 tttggagcaa atatggaccg cgtccttcat tttgtactgg cacttgccgt tgttgcgatt
    18421 ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta tccgttatgt tattcaactg
    18481 cttgttatcg aagtgttact ggcgtggttc ttcctgaact ccgacgttgg tttgggcttc
    18541 gtgaaaggct tctccgaaat gtttgagaaa ctgctcggat ttgccaacga agggactaac
    18601 ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct tcttcctgaa agtgctgtgc
    18661 ccaatcgtct ttatctctgc gctgatcggt attctccagc acattcgcgt gttgccggtg
    18721 attatccgcg caattggttt cctgctctcc aaagtcaacg gtatgggcaa actggaatcc
    18781 tttaacgccg tcagctccct gattctgggt cagtctgaaa actttattgc ctataaagat
    18841 atccttggca aaatctcccg taaccgtatg tacaccatgg cagcaacggc gatgtccacc
    18901 gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg aaccgaaata cgtcgttgca
    18961 gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt cgctgatcaa cccttaccgt
    19021 gttgatgcca gtgaagaaaa cattcagatg tccaacctgc acgaaggtca gagcttcttc
    19081 gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg ccattatcgt tgccgcaatg
    19141 ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac tgtttgctac cgtgactggc
    19201 tggtttggct acagcatctc cttccagggc atcctgggct acatcttcta tccgattgca
    19261 tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg gcagtatcat ggcgaccaaa
    19321 ctggtttcca acgagttcgt tgcgatgatg gatctgcaga aaattgcttc cacgctctct
    19381 ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct tcgctaactt ctcatcaatc
    19441 gggattatcg caggtgcagt taaaggcctg aatgaagagc aaggtaacgt ggtttctcgc
    19501 ttcggtctga aactggttta cggctctacc ctggtgagtg tgctgtctgc gtcaatcgca
    19561 gcactggtgc tgtaagacca tacataaaaa agccggggat aattccccgg ttttttacgc
    19621 ctgttaatca gctaatggct gcgggcgacc aatcaaatac ccttgcagat attgcacccc
    19681 gagcttatgc aatagcgcct gctgctgttg cgtctcgaca aactccgcga ccacactcaa
    19741 tgacttcgct ttcgccagat cggtaattga tctcacaatc atcgcatcca gcgtgttcgt
    19801 gacgatatct ttcacaaaga cgccatcaat tttgatgata tcagcctgca aacgctttaa
    19861 ccgttcgtag ttggcatatc cggtgccaaa gtcatcaatc gcaatccgga aaccaaactt
    19921 atgcagctgc tcgatgttgt acatgctgct ttctgcgtta gaaaacgcct gctcctcggt
    19981 gatctcaaga atgaccgcct gcggggagat gtgataacgc ttaaacagac gaataatccg
    20041 cccggcaata ttcttttgca gcagcgtgag cggcattaaa ttgactgaaa agcgcgggcc
    20101 ttttttgtcg caaggatgtg ttgccagcca cttcaacagg gattccagca cttgcaaatc
    20161 aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga aacttatccg gggtcataat
    20221 gccgccgtca tatttcagtc gcgcgaggat ctcatcataa ccttcaccct ctttgttgcg
    20281 aattggctgg gcgtagagca gcaaatcacc ctgatctaac gccgtacgaa tggtattcag
    20341 caatagcacc tgtttagtgg tctgcccgga aaccatctcc tctctgctat ccagcgccag
    20401 cacatggtga tgtgcgcagg attgctccgc cagccagctt aactgcccca acaagggttg
    20461 cagggtttcc tgatttccat caaaacgccc ccaggcagca ccatagccca tatccagccc
    20521 ggtattgttc cagtgaattt gccgactatt caggatgtta accatatgct ggagtcgccc
    20581 ttccgtttcc ggtccactca gcactaacag cagttcacta cccggcaatt gatacaactt
    20641 ttcgttttcc tgcatcaacg gcagcagcgt acggtaaatt gatcggatac agtgaacgcg
    20701 catcattaag ccgtaatgac gactcataaa ctcaagatta tcaatgcgca ggcagcaaaa
    20761 actcttgccc gcctcttgct ccggcgcttg ttctaacgca cgaaagttgg gtaggagcgt
    20821 taacggatcg gtcagcgcct gcaaatgcca gcggcgatta agccattcac tgcgatgata
    20881 aatgcgcacc atatagagca ggcaaacgct aaaggaaatc agcaccgcga gaataaatgc
    20941 cagcgaatat tcggttagta ccccttgcaa aaagttctgg ttgtaattca gcaggcaaag
    21001 cgttgaaaca gcccaggtga gatttaaaaa cggatagcga agcttaccga caccgagggt
    21061 aaagatgata aagaaaacag gtaccagata accagcaata aagtcatttt catacggtgt
    21121 gcacatcaga agtagcagca cgctaagagc tgccagccag cttaaggtaa atgcgcgttt
    21181 ctctttgctc aacgacggag cgatatccct acgccacaat atctgtgcaa agtggggact
    21241 tacaatcatg cgggcgagat agtaaaaaag catgttgtaa atcagcactg cggtgaaaag
    21301 gcttagcaaa tcaacgaccg tgaaaatggc atccgcatcg ccgaaaaagg tagatatctt
    21361 tagcggaaaa tcaaagaaat ttcccacaag atacatgctg catttgatgc caatcggcgc
    21421 caccaaacca agccagaaaa gacgttgcca gacatatcgg ctggtcaatc cataacgcca
    21481 acgcgtcccc agctgccagc gtaatattgc gcaggcacac aacaccacaa acgtctggct
    21541 gccgagcatg accgcggtct gtaaaaaaga gagatgaaag ttccacagat tggtgcaaaa
    21601 catcccgcat aaaacgggga cgacaccgcg ccagccaaag ataaacaaga ttgacagcat
    21661 tacgcacagt ggcatccacg ccagaaagat ataactggag tggataatgg caagtggcga
    21721 aataaaacgg gatagctgaa taagtaccac ggtgagcgta aacgctagtg tgaatatctt
    21781 gatatttttt atcaggttat gctccacaaa catgagtaca ctactctcac tgatcggggt
    21841 tttcaggcac acaaataata agtttattcg catcgcgaag caaacagggg ccagcctgtt
    21901 gccgattttt catccaatga tgctttcacc aggcggcgct aacccggacg tagactgtat
    21961 tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt tacagcgagg gtttgaaatt
    22021 tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca
    22081 gctgagctat agccccacga tgcgtttacg taccaaattt gctgggtgca aaatttggtg
    22141 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    22201 gctatagccc catcacgtaa agcttgtcga gttgacgggc ggcatcatat gaattccgcc
    22261 cgaatgtgtc aacggcaaat tgccacgtgt agtttcaatc gctgaaaaat caggcaaatg
    22321 aacaattttg gaaaaccgct cgcattcagt agttattcat gtcacggttt cctgtaaagt
    22381 ggtgttataa aatgaactac taatagaccc acatacattc agggaattgt tatgttcaag
    22441 gagaggatga cgccagatga acttgccaga ctgaccggct atagccgcca gaccattaat
    22501 aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac caggcgtcca gggtggcaaa
    22561 gctcgactgg ttcacgtcaa tgaacaggtt cgtgaatata ttcgcaatgc tgaacgtcca
    22621 gaaggtcagg gagaagcgcc agccctttct ggtgatgcgc cgcttgaagt gttgctggtg
    22681 acactggcga aagagatgac gccagttgaa caaaaacagt ttacatcctt gcttctgcgg
    22741 gaagggatta tcggattgtt acaacgctta gggattcgcg atagcaaata atatgaaaag
    22801 attacgcaat aaaatgacca ccgaagaact ggctgaatgc ctcggtgtgg ctaaacaaac
    22861 tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa aaatttcccg gcgtgaaagg
    22921 cggtcgtgcc agacttattc tggtcgatac gcaagtttgc gagtttattc agaacacgcc
    22981 agccttccat aacacaccga tgcttctgga agccgaagaa ccccttgccg aatacgcacc
    23041 gggtattcgc actcctgctt atcggcaaat catcagcgca atagacaaca tgactcacag
    23101 tgagcaggaa aaagttgcgc aatttttgtc acgcgaagga attcgtaatt tccttgctcg
    23161 tctcgacata gatgaatcag aataaataaa aaacggcagg ggtatctctc ctgccgttta
    23221 tcttttatgc gctaattatt gctgattttc gcgttcagca ataaaagcca gcgctttgtt
    23281 gatacgctcg atactgcggg tcttaccgat tgcgtgaacg gtaacatcca gcgccggaga
    23341 ctgccccgca ccggttacgg cgacacgcag cggcatacca actttaccca tacccacttc
    23401 cagctcatcc gccgtcgcct gaatagcgtg atgaacgttt tcagcggtcc agtcagtaat
    23461 cgcagtcagt ttgtcacgaa ccacttccag cggctgacgc gctaccggac gcagatgttt
    23521 tttcgcggcg tcggcatcga actcagcaaa atcttcgtag aaataacggc agctctgtgc
    23581 catctctttc agcgtcttgc agcgttcgcc cagcagtttc accagatcag ccagctgcgg
    23641 gccgttacgg gtatcgatat tttcctgctc aatgtgccat tgtaagtgag tagcaacata
    23701 ctccggcggc agcgcgttaa tgtagtgatg gttcagccac agcagcttgt cggtgttgaa
    23761 cgcactggca gatttgctga cggcattcag agtgaagtat ttgatcatct cttcacgagt
    23821 gaagatttcc tgatcgccgt gggaccagcc cagacgcacc agatagttca gcagtgcttc
    23881 tggcaaataa ccgtcatcac gatactgcat tacgctgacc gccccgtgac gtttggacag
    23941 ttttttaccg tcatcgccgt tgatcataga aacgtgcgcg taaaccggta ccggcgcttt
    24001 cagggcttta agaatgttga tctggcgcgg cgtgttgttg atatggtctt cgccacggat
    24061 aacatgggtg atttccatat cccagtcatc aacaaccaca cagaagttat aggttgggga
    24121 accatcggta cggcgaatga tcagatcatc cagctcctgg ttgctgaact caatcgggcc
    24181 acggatctga tcgtcaaaaa caacagaacc ttcctgcggg ttagcaaaac gtacaacgca
    24241 cggttcatca tcagcatgat gctcatggct gtggcggcag cgaccgtcat aacgcggctt
    24301 ctcacctttc gccatttgct cttcgcgcag cgcttccagg cgctctttag aacaatagca
    24361 tttataagct gtgccctctt ccagcatctg atcgatcacc gcgttgtagc gatcaaaacg
    24421 tttggtctgg tagtacggac cttcatccca ctccaggctc agccagttca tgccatccat
    24481 aatggcttcg atagcttccg gcgtggaacg ctcaagatcg gtgtcttcaa tacgcagcac
    24541 gaactcaccg ccgtggttac gtgcaaaaag ccaggagtaa agagcagtac gcgcgccgcc
    24601 aacgtgcaga tagcctgttg ggcttggcgc gaagcgagtt ttgattttca tgaaatggcc
    24661 ttacgtttag aaagatgccg acaaccggca aatcctggaa aaattgagtg ggcgatattc
    24721 tatcactccc gcctgattcc tcaatgtagt tcgggttgca accttacgca ttattgtttt
    24781 cgttgacaaa ttgcgcaaca cggtctgttt tgcgatcatc ttgcttaaat ttacgacgaa
    24841 cgaacaattt ctttagaaaa tgcgttgact cattttgaac tctccctata atgcgactcc
    24901 acacagcggg ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc
    24961 ggttcgatcc cgtcatcacc caccaactac tttatgtagt ctccgccgtg tagcaagaaa
    25021 ttgagaagtg ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc
    25081 ggttcgatcc cgtcatcacc caccacttcg ggtcgttagc tcagttggta gagcagttga
    25141 cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccaatgta aaaaagcgcc
    25201 ctaaaggcgc ttttttgcta tctgcaattt gcgaaattgc ctgatgcgct tcgcttatca
    25261 gaccaactat gtccggcagt tcccgtgcct ctcatctact gcgccaacgc cgccagcagt
    25321 ttaaccagcg cgggcgtcac ctgctgcgtt tcataaacaa tatataagtc tgccgggatg
    25381 cgttctttga gcggacggaa tatgacaccg ggccagttca tctgcgcgta gctgtccgct
    25441 atcaatgtga taccaatacc catactgacc atggcgagta ccgtttgcgg ttcattaact
    25501 tcgcgaataa caaccggtga aaatcccacc tgctggcaaa ctcgctgcaa aaaatcccag
    25561 tcagtgtaca cgggcggcat tgtaacgaaa tactcgtcac gtagcgcttc cagcgggacg
    25621 gtggaaaatg atgagagatg atgctcttca ggcatcgcca ccagaaacgc cgattcatgc
    25681 aaccgtaagc tggtaaaacc agtcggtggt tctgtcgcca ttcgccagat cccggcatca
    25741 agttcgcggc gttccagcaa ggccatttgc atcgcaggca tcttttcgcg aaacagaact
    25801 tcaacgttag gattttctct aaggaatcgc cgcataaccg ggcgcatccg tccccacatt
    25861 gccgttccca caaccccgag ttcaatccgc cctgcttctc cccgacctat ttgttcaacc
    25921 cgagccaata cattatttgc attcaccagc aatcgacgcg attcttccat caagattttg
    25981 cccgcgtgtg tcagtacgac gctgcgcgaa tggcgaataa aaagctgcgt gccgagttga
    26041 ttttccagct ctttaatatg aatgctgagc ggaggctgag acatatttaa acgcgctgct
    26101 gcgcgaccaa aatgcaactc ttccgctacg gcaagaaaat aacggagcaa cttaagatct
    26161 gttctgtata cgcgtt
//
LOCUS       DAZGAP010000064        25998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGAP010000064 DAZGAP010000000
VERSION     DAZGAP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.064"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(23..4483)
                     /gene="mukB"
                     /locus_tag="ACHLAM_002208"
     CDS             complement(23..4483)
                     /gene="mukB"
                     /locus_tag="ACHLAM_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO7853729.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4483..5187)
                     /gene="mukE"
                     /locus_tag="ACHLAM_002209"
     CDS             complement(4483..5187)
                     /gene="mukE"
                     /locus_tag="ACHLAM_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO7853730.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5168..6490)
                     /gene="mukF"
                     /locus_tag="ACHLAM_002210"
     CDS             complement(5168..6490)
                     /gene="mukF"
                     /locus_tag="ACHLAM_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO7853731.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6487..7272)
                     /gene="cmoM"
                     /locus_tag="ACHLAM_002211"
     CDS             complement(6487..7272)
                     /gene="cmoM"
                     /locus_tag="ACHLAM_002211"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO7853732.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7408..8187
                     /gene="elyC"
                     /locus_tag="ACHLAM_002212"
     CDS             7408..8187
                     /gene="elyC"
                     /locus_tag="ACHLAM_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO7853733.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKSSSGEPRQE"
     gene            complement(8164..9057)
                     /locus_tag="ACHLAM_002213"
     CDS             complement(8164..9057)
                     /locus_tag="ACHLAM_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO7853734.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDTSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9211..9957)
                     /gene="kdsB"
                     /locus_tag="ACHLAM_002214"
     CDS             complement(9211..9957)
                     /gene="kdsB"
                     /locus_tag="ACHLAM_002214"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO7853735.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9954..10136)
                     /gene="ycaR"
                     /locus_tag="ACHLAM_002215"
     CDS             complement(9954..10136)
                     /gene="ycaR"
                     /locus_tag="ACHLAM_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO7853736.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10188..11420)
                     /gene="ycaQ"
                     /locus_tag="ACHLAM_002216"
     CDS             complement(10188..11420)
                     /gene="ycaQ"
                     /locus_tag="ACHLAM_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO7853737.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            complement(11457..12443)
                     /gene="lpxK"
                     /locus_tag="ACHLAM_002217"
     CDS             complement(11457..12443)
                     /gene="lpxK"
                     /locus_tag="ACHLAM_002217"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO7853738.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12440..14188)
                     /gene="msbA"
                     /locus_tag="ACHLAM_002218"
     CDS             complement(12440..14188)
                     /gene="msbA"
                     /locus_tag="ACHLAM_002218"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO7853739.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14225..16489)
                     /locus_tag="ACHLAM_002219"
     CDS             complement(14225..16489)
                     /locus_tag="ACHLAM_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO7853740.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVAHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSQLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16696..16980)
                     /gene="ihfB"
                     /locus_tag="ACHLAM_002220"
     CDS             complement(16696..16980)
                     /gene="ihfB"
                     /locus_tag="ACHLAM_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO7853741.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17140..18813)
                     /gene="rpsA"
                     /locus_tag="ACHLAM_002221"
     CDS             complement(17140..18813)
                     /gene="rpsA"
                     /locus_tag="ACHLAM_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO7853742.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18924..19607)
                     /gene="cmk"
                     /locus_tag="ACHLAM_002222"
     CDS             complement(18924..19607)
                     /gene="cmk"
                     /locus_tag="ACHLAM_002222"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO7853743.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19780..20544)
                     /gene="ycaL"
                     /locus_tag="ACHLAM_002223"
     CDS             complement(19780..20544)
                     /gene="ycaL"
                     /locus_tag="ACHLAM_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO7853744.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20713..21996)
                     /gene="aroA"
                     /locus_tag="ACHLAM_002224"
     CDS             complement(20713..21996)
                     /gene="aroA"
                     /locus_tag="ACHLAM_002224"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO7853745.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQDNYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNMMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRIAMCFS
                     LVALSDTPVTILDPKCTAKTFPNYFEQLARISQAA"
     gene            complement(22067..23155)
                     /gene="serC"
                     /locus_tag="ACHLAM_002225"
     CDS             complement(22067..23155)
                     /gene="serC"
                     /locus_tag="ACHLAM_002225"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO7853746.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSNFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23354..24046)
                     /locus_tag="ACHLAM_002226"
     CDS             complement(23354..24046)
                     /locus_tag="ACHLAM_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO7853747.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFIILALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESVDYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24176..25936
                     /gene="ycaO"
                     /locus_tag="ACHLAM_002227"
     CDS             24176..25936
                     /gene="ycaO"
                     /locus_tag="ACHLAM_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO7853748.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         6301 a         7081 c         6322 g         6294 t
ORIGIN      
        1 cggcattgct gccgccttca ttttaactcg cttggtaagg cgcttcgtca cttcctggaa
       61 gcgtttcagg gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat
      121 tctggaagac tttacgcacc agtttatagg tggtgccttt ttccggactg atattttccg
      181 gcgctgcgat gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag
      241 aacgagcatc cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt
      301 taccgcgcag gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca
      361 taccggtacc aatcgcctca ccggtcgaca gagcgccaga ttctgcgcgc agccagccat
      421 cagaaccacg gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt
      481 cgccaatggt ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca
      541 atttcgccag cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct
      601 cgtgctgttc agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacattca
      661 gacgtacgct gtttacctga ccgaacgata cgttctgcaa accctggttg agcatacgga
      721 tacggttctg ttcgcgctga atggttttgc gaataatgtt cgccacgctg cgggaactga
      781 tcgccagttt ctgttcacgg gaggttaact cttcggtcag acggctaagt tcgatctcca
      841 tctgttcgat agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac
      901 gcagatgctg ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt
      961 ccgacatccg cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac
     1021 ctaacgcctt atccgacatg gaacgcaaat catcagcgga gagataagcc agctcacgac
     1081 ggtgtaagcg gcgttcaacg ccgttatctt tcaccatgcg catgactgca caccagcccg
     1141 ctttcgcggt cactacctgc tcgcgcatct caaagtaatc acgctccagc ttgcgcagtt
     1201 tgcgggtcag gttgtccatc tccgcttcgc agaaggtaag cgctttttcc agttgattgc
     1261 ggcgtgaacg gttattgctc agttgcgcgt gcaactcgtc acggcgaata cgcgcccgct
     1321 cttctgcccc gctatcagca cgcacgccga tatcctgcaa ttcacgttgc agatcgttga
     1381 gtagctcttt tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac
     1441 tcaactgcgc tgcgtgtccg cgcaacgctt cacgcgcgcg ggtacgctcc gcttccgcct
     1501 gttccagacg ttcacgcagt ttttcgttga gatcgctgtt accgctcagc atttctgccg
     1561 agtcagagta gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct
     1621 gacgggcatc gcgctgcatc tgctgagagt acgcgtaatc ttcttttaac tgttcgaact
     1681 gttccgggtc gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa
     1741 actgctgaac aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga
     1801 cgcgatccgc caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg
     1861 ccgtaacgcc ctctttcgcc tgctcaaatt gaatacgctg ctgctggtta tcattttcat
     1921 gattactcag cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttcggctt
     1981 ccggatcaga ctcaaacgcc accgccagat gactacctat aaagcggctg aacgcctgat
     2041 gcagacgctg ggttttctgt acatcaaaag agagcgtggc gaagcgttcg gaaagcactt
     2101 cacgctcggc atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggaa
     2161 cttccggaaa acgtgagtaa cgccactgac gatcggcgat tttcaccact accgcttttt
     2221 ccagctcatc aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcagat
     2281 agagatcttc cgggcaatcg gtcaagcctt ccaggtgttc ggttacctgt gacagatctg
     2341 gcaccacgat ggcgtggcgt gacgggccat acagtgccga gaagtacggc gcatcttcca
     2401 gactcacgtc gtcataaatt tcggacagca gcacgccgcc aaaacgctcc gccagcgcat
     2461 tcaaacgctg gtcttcagaa ccacctggct ggcttaaacg ttcgatctct tcatcgacgg
     2521 cgtttttgcg cgcgcctact tcatcgcgtt caacaatggc ttcgcgctca cgctccagca
     2581 actgttgcag atattcagtg acgtcctggc tggaggtgaa ctcttcgccg cactgttcgc
     2641 tcaactggtt gagactgttt tgcgctgcca gccaaaccgg tgcacgctgc atcaaactct
     2701 gaatgcgaga ctgcagctgt tcctgttcct ggcgcagtgc catccgctct tctcgggcgt
     2761 tagagacgct atcggaaaga gaggcaatgc gtgcttccag ctcctgatgc agagcttcca
     2821 gttcatcaat atcaaaattc ttgccctgac gtttgcagaa atctgccagc agacgctcag
     2881 cttcttgctg ctcacgcaga cgctgttcca gttcgcttaa gcgcatccgc aacggctgaa
     2941 cctgctctgc caggtgacgc tgatcgaccc cttcgcgcaa tagttcgcga gcgacatccc
     3001 acgcctcgtt acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa
     3061 actggctgtg cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcgg
     3121 tcgcttccag ctctttcgcc tggaaggttt ccagccattc ggcagcgctg tcggcggtta
     3181 agtccggcag atggcacagt tctttggcac gattaagcgc agcaatcgcc tggttatact
     3241 ggatcgcgcg cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca
     3301 gctcgtccac ttccagttcg gcagcttccg cacgagcctc gttctcttcc tggcgttcga
     3361 tggcttctgc caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg
     3421 cttcgtagcg ttcgatcttc tcctgctgac gcagtgcggt ttgcaccagg ttcaggtgat
     3481 cgctggccgc ctgataatcc gcttccagat cgccttcggc accgttgtgc tcagccagct
     3541 ctcgcgccat gtcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta
     3601 gctcgcgacg aaactccagg gccttatcga gatgaacacg gcgctcgttg gcgtgacgca
     3661 tgtagtccgc cgccacgtag ttggtggctt cgctgatcag atgcttaaac aggtcacggt
     3721 ctgactgggt gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca
     3781 tgtcctggaa cgctttccgc accccactgt tttccggcaa caggtagtcg cgcagagaac
     3841 gagtaatggc gctggagatc ccgccataca gcgaggcttc gatcagacga tagaatttac
     3901 tacggtcaga cgctgagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt
     3961 ggtaatcggt aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt
     4021 ctttcagctc gttaagcggc agcacgcgcg cctggcgttc gttcagagtt tcggtcacca
     4081 gttgtgtcgg ctgcaccgac atcggcaggc cctgaatggc aaatggtttg atatccactt
     4141 tacgatcgcg tccggcaacc tgttgcagac gcacaccgac cactacacgc tggtggcgcg
     4201 agttaatggt gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt
     4261 tatcgcgcga accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca
     4321 ggtcggggat aagcgccgta acgaacgctg ccatggtggt ggatttaccc gccccgttac
     4381 cgccagaaag cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc
     4441 agttaatcag cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct
     4501 atctggctga ctctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc
     4561 ttcgccatca cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc
     4621 ggctccgaag cggaacaccg attcggtaat gcggaacttg ctgctgtcgt ggcccataaa
     4681 ccacaccata cccaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt
     4741 ctgccggtca acgtctgaac cggttgaacg gttgttcacc agtttcagca gtttcgcttc
     4801 atcggccagg gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc
     4861 cagccgttcc gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc
     4921 cgacaagacg gaacgaggga tcagcgtggt ggaacgtggg cgtagataga agaacccttc
     4981 cggtgcacga ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa
     5041 atccatcagg aatgcatgat tatccagttc gtcgaggcca atatgacggc ctgaacgtaa
     5101 ggcgctatcc agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccggcat
     5161 cacttgttca atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg
     5221 gctgccattt cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct
     5281 gatcaataac aatgcgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt
     5341 cgcgtaccac cagaccaaga tccaacggca cttgtctggt tttgtacacg gcaagttgtt
     5401 cttcgatgat cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtac tccagatcct
     5461 caggaagttc cccagtcact tcttcatcgc gcagtaccat ctcttcgtca cgcatatcca
     5521 gcagacgatc ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg
     5581 actgacgtaa ccgctgagca aagacgcggt ttttatccat atcgatcgca gtacggataa
     5641 atttgtgtac gtggcggtcg tagccaatcc acaagtctat ggattgctgg ccccaactga
     5701 taatacggtc gagtttgctc tgcagatcga acaccagacg atcgacaaaa tgcaggtcgt
     5761 catgggtcat cgtcgcatcc tgaatgcgca acagattagc ctgcaattta tcgcctgccg
     5821 cttccagcgt atcctgcaat tcacgcagcg ttccggaagt ttctgaaagc aacaattcac
     5881 agctggaaat cgccgcccgc cagtctttgt tcaacaactg ggcgatatca tccttcacct
     5941 gctgctgctg ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta
     6001 ccgaatattt cagtggcgca taaacattac ggtgccagtg aaattcatca ccgccctctt
     6061 cggcggcatc tgccgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaaggc
     6121 gcagcgtaga aaactcgcgc tgacggatgt aatagtcagt aatgccgatg ccgagcggcg
     6181 tcagacggta aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt
     6241 gacgcaccat gtcgttgatc gcgttgttgg cgcgcacgcc gatggtttcg ctggtttgct
     6301 caaacgcatc actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat
     6361 ccagacgctc gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca
     6421 gcgagatgga gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa
     6481 attcactcat actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc
     6541 cagggtaata tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata
     6601 gcagtcgcgc tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt
     6661 acccataatt tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg
     6721 ataatctggc gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa
     6781 attcccggcg accatgttat gcatcagcaa gccgtgcgca ttgtagaaca ttaacgacaa
     6841 cacaccgcct ggacgcaaca ctgaccacag ggtctgtaat acgctgcggg gatcggccac
     6901 ccactcgagc accgcatgga acaatatcag atcaacgggc gtttccaaat gtgaagcaac
     6961 atcctgagcg gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc
     7021 ctgttttgcg cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg
     7081 ctcagccatt ttgattgcgg tctgcccttc tccaccgcca gcatccagca cacgcagttt
     7141 ttgcgggccc atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag
     7201 ctgccctttg gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt
     7261 gcgatcctgc attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt
     7321 ttgtcacagc cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct
     7381 atatggttaa aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat
     7441 atgttgctgc cacttccgct aatgttgttg attattggcg ctggcctggc gctgctgtgg
     7501 tttagccgtt ttcaaaaaac cgggaagatt tttattagca ttgggtggct ggcgctcttg
     7561 ttattgagtt tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg
     7621 acatggaata attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg
     7681 aacccgcagt gggcaccaag ctcgaattta atcaataaca gtctgccccg cctgaatgaa
     7741 ggtattcgct tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca
     7801 aaaaccaata cggtgagtac agccgaagta ggtgcccgag ttgcgcaatc gctgggcgtg
     7861 ccgcgcgagc aaattatcac tctggatttg ccaaaagata ccgaagaaga agctgcggca
     7921 gtgaaacagg cgattggtga cgcccctttc ctgctggtga catccgcgtc tcatttgccg
     7981 cgggcgatga tcttttttca gcaggaaggg ttaaatccgc tgccagcccc ggcgaatcag
     8041 ttggccatcg actcgccgct taacccgtgg gaacgggcaa tcccttctcc ggtctggttg
     8101 atgcatagcg atcgtgttgg ctatgaaacg cttggccgta tctggcagtg gctaaaaagt
     8161 tcgtcaggcg agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa
     8221 tcgtccggta ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca
     8281 gatgaacgac tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag
     8341 agaagtatcc attagtcgga acagttcgta ttcccgcggt gcccaaagca ttagtcccgg
     8401 cccgaccatc gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa
     8461 gttaccgtga atcagcacac aattgtcgtt aaagccttca aataatgccg ggagacattc
     8521 gcgagtacga aacaggatcc gcttatcctg catcgttaga ccggtgttat tgaactgatt
     8581 gagcgtggtc catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg
     8641 agtattgtcg accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc
     8701 ttcaacgatt tgatctttga gttgttccca gcgttctggt gttcgggctg gtgcctccac
     8761 cgaaacacca cgcatccgct ccagtagcag gacatcgggg ccggggtgtt cttcatgcgt
     8821 catcacacca taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt
     8881 ccatgccagt tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg
     8941 gtttccctgg ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc
     9001 aatacgactg agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca
     9061 gattaccctc ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg
     9121 aatgagatca attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc
     9181 caaaattggc tggagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcgag
     9241 atcttcaggg gtatccacac ctgtgccagg aacttcctgg gcgacagcaa catggatttt
     9301 ttcgccgtac cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgg
     9361 ctgccagttg acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg
     9421 caggaagtta tcgccaacgg tttcaaggcc ttttgcaaaa cgatcacgat cccaaggaat
     9481 ggtggcgcga gagaagtaca gtgcataccc ttcagcgtcg aggaccactt tcaccgcatt
     9541 cgggttaaac gcttcctccg cattgtggat tggcaccgcc agagtcgcca tacccacctg
     9601 acgctgagcg aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc
     9661 accctgcaca ttaacgatca cagtgtcgtc gctgaatgcg catttttcga caacttccgc
     9721 caatcgttct gtacctgact gatgatcggc gcgcgtcata catacttcgc cgccagcggc
     9781 ttcaacagcg cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga
     9841 ttcgcgcgcg cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg
     9901 tttaccgggc agacgcgtgg acgcgtaacg cgcgggaata atgaccacaa aactcatgat
     9961 ttactctcat cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg
    10021 cgcagcggga aagccaggtt gtccagtttg caaataagct cttgtttttc ctggttatac
    10081 caaagttttc cgttgcaaac cgggcaggca atgatttcaa gcagacgatg atccatagtt
    10141 cctccagatg gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg
    10201 tctatttccc agccggcgcg gcaatcagta aagaggcctt gcgggcagcg tcctaatgtc
    10261 acccgcgttg cctgctgcca gttagcgaaa tcagtaatcg cctgacgtaa ccctttttgc
    10321 agcgttgtcg ttggtttaat gccttcttgt aaccacagag agataacttc aaggatgcct
    10381 gtctggcgat gcattttggc atccattcgc ccaactaatt gcccacgatg taataacggc
    10441 agaacaaaat agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta
    10501 aaatcaaaaa gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt
    10561 accgcgctgt gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa
    10621 tcagcatgca gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt
    10681 tcagccctcg cttcacgcca cgccgccagt gccggacgtt ttagccggta gtaatctgcc
    10741 agccactgtt cgcgaaatat acccaggcta cgcgcactgt tatccagcat gatgatttct
    10801 gcttctgctt gcgaaacgag atcgcgctca tcattccact caggcatgac acggtgagtt
    10861 aaatcataaa cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat
    10921 aaaccttcca gatgtcgttt atgcggcttc cactcccacc agccgctcgc ccctttacga
    10981 ggatgctcaa aatcggctga acgtactggc cccttatcat gaatatgctg aattaactgt
    11041 gcaatttccg ccgcatgttc ctgcatccag gcgtctttat atttccagcc cattttttca
    11101 ggtgccagca tgcggtggcg aataagacga aaatcgctac gcggcataaa gcaggcttca
    11161 tgcgcccagt attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga
    11221 taatttccca gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta
    11281 tcgatttgca gcaaggacat gcgggagatc gttgccggaa tatcccccaa cgacgctcga
    11341 cggcggggtt tgtttaacag gccttgcgcg gcgaggtgaa gattacgcgc atcagcaaga
    11401 gagaggtgcg gcagcgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag
    11461 ttgccagaag ccagcgaggt tagttgcgca agcagtttcg ctggttcatc acctgaaagc
    11521 tgtgcgtcaa caggcaaata ccaccaattt ccttcggcaa aggcccggca tttcaccgca
    11581 tctttttcag tcattaccag cgtttgcccg gcgtttacca acgcgctgac atccgcatgg
    11641 ttcaaagact gatgatcggc cagcggtaca catttttccg gttgtacgcc gcacatcttc
    11701 agcgtggcaa aaaagcgcgg cggatgtcca atccccgcca tcgccactac atgttcaagc
    11761 tgagcaacgt cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc
    11821 atggggattt caccgctgcg ggggacaccg ccgttgacga ttaccgcatc aaccgacttt
    11881 aagcgccccg ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga
    11941 cgcacaccat caataacgac aatttccaca tcacgcgcca gacgataatg ctgtaaacca
    12001 tcgtcggtta cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcgtca
    12061 gaacgcacgg gagaaaccgc aacaggcgcg ccagtgcgtt gataaatcaa cacaggttca
    12121 tcacccgcct gtgctgtggt ggtatctgcc gacaataaca gcggataaga ttcagcctta
    12181 ccaccatatc cccgcgatac aacccccacg cgaataccgc gctgttgcag ctgttccacc
    12241 agccagacaa cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca
    12301 accggtacgg gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg
    12361 ccactcacca ggccatacaa ccaggagagt ggcagcaata gccgccataa aggggattca
    12421 ccagaccaga ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc
    12481 cacggtgctc aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcgacaa
    12541 ccacgatttc gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac
    12601 ggtttttctg caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg
    12661 ccgaggtagc ttcgtccaga atcagtatcg ggctatcacg caacaaggct cgagcgatag
    12721 caatacgctg acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga
    12781 gaccgttatc catcttattg atgaagtcca tggcgtaggc catacgcgcc gcttcttcaa
    12841 tttgctcacg gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt
    12901 taaacagatg gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat
    12961 actcgcgcag atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg
    13021 tgatcaggct ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcaaccgtct
    13081 tccctgccgg aattttcagg ttgatgttac gcaatgcggg cacatcacgt cccggataag
    13141 taaaggtgac attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt
    13201 cgtctttctc ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc
    13261 gctggaactg ggcgttaacg ttggtcagcg atttcagcgg acgcataagt gcaatcattg
    13321 aagagaaaac aacggtaatc gtaccggcag tcaggctatc catgacactt gggaagcttg
    13381 ccgcatacag aacaaacgcc agcgccaaag aggcgatcag ctgaatgatc ggatcagaga
    13441 tggaagaggc tgaaaccatt ttcatcccct gaagacgcat tcggttgctg actttatcga
    13501 agcgtttcgt ttccacttcc tgaccaccga aaatcaatac ttctttatgg cctttcagca
    13561 tttgttcggc gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc
    13621 gaaaacgctt cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa
    13681 tgatcgacag ttgccaactg taatagaaca tcatgatgaa caggccgatg atcgacgcac
    13741 cttcacgtac aacagtaatc agtgcgccgg aagaagaaga agcgacctgt tcggaatcgt
    13801 aggtgatacg tgacaacagc gtacccgttg actgtttgtc aaagaatgaa accggcattc
    13861 ccatcatgtg accaaacagg cggcgacgca tagtcattac cacctttcct gatacccagg
    13921 agatacagta gctggagata tagctggtga taccacgtaa aatcatcagc ccgatcacca
    13981 ccagcggcat ccacatcagc acggagcgat ctgttttacc aaagccatca tcaagaagtg
    14041 gcttaaggag cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg
    14101 ccacgatcag acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aatgtctgcc
    14161 acgtagagag atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca
    14221 tattctaccc gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt
    14281 gctctctgag gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgaccct
    14341 gatgtggagt atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg
    14401 agggcagtcg ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc
    14461 gctgaattaa tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagtaatg
    14521 ttgcctgcat ttgctgccag taacggctta gcatcttttg ttcagcgggg gcttcaatat
    14581 caccggttag aagaatgcta ttcgtcccgt catcaatctt aaccacacag gaatggttat
    14641 ttcctttatc gttgctacct tgtaaaggcc agtgcgcgct gaaacgcaat ccttgccatt
    14701 gccacgcttc gccacgcaca cagggctgat gatgttccca gtttaactga cttcggatcc
    14761 ataacatcgg ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat
    14821 gactcaaaat aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt
    14881 gttgcccact atccccttcg ggccaggcca gtcccgtgtc atagagaatc gctttgccgt
    14941 ttcgcgcaat gaccattgcc agcccttgcc cgacatcaag catgtacact tgccactcgt
    15001 caggtcgagg tttttgccac agcggcaagc acatcagcaa gcctacagcc acacacattg
    15061 ctggcaacgt tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg
    15121 ttagccattg ccaacgttca gcaatgttga tccacccttc cggcaaactc tttaaccccc
    15181 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgttcc agaataaacg
    15241 gcccgcttaa atgcgcaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
    15301 ggggaattgc aaatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
    15361 tttgcacggg cataagcagg agtgtgattc ccagttgtaa atgaatgagg gaaacaactg
    15421 cacgcaatac cggcggcagt tgccactccg gacagggaaa ccactggtac caaaaaatca
    15481 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
    15541 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccat tgtcgcccac
    15601 ttaacttaag cattccccac atggcaagcg ccaccacggt acgcaatgca ggaggttgca
    15661 tcccagtcag ccaggcataa aaagcagcac agcagattcc gccaattaat ggcatttgcc
    15721 agtggatcca gcgcccaggc agaaaaactt gcccaccgcg aatgagtccg gcagccagca
    15781 acgccgcaaa agcgatatgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
    15841 gcatgatatt tttgatttct ttgggaacgg ataaccgttc ccccatacct aaaccaagaa
    15901 taaccgcgtt ccacatatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
    15961 gcaggctaca attcggttca atgacacttg cctgcagaaa gcggccggtg agcggctgat
    16021 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaactg
    16081 cacgaacttt gagtttcatt gaccacagtt gtccagcaca aaccgcttgc ggaagatatt
    16141 cgccatacag cacgaggcct ggcgcaggga atatacgtcg accttgtaga tgagtaattt
    16201 gaccgtaatg agtggtcata ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
    16261 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgctaatatg ccccaaacga
    16321 aaaacagcaa cgtcagcgcg atataacgga cagttttaaa agggataaat accagtacgc
    16381 aggcgaagac ggtcagagac gcaagggtta atgtcccagg caattggggc aaaatcagca
    16441 acggaaaaat tccgcaaatt atgcatacgc cgactgtcgt tattttcatg cacccctccc
    16501 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtaggagttt tgcgttcccc
    16561 gaggcacgtt cagaagaatt taacgagagg gtgaaaactg ttcaaaactc tgcgaggctg
    16621 cttccagttc cagattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
    16681 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt tacctggttt
    16741 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
    16801 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
    16861 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
    16921 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
    16981 gattcctccg gttccttaaa ggcaaattta gcggctgctg ttgctgcatt gattaagggc
    17041 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
    17101 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
    17161 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
    17221 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
    17281 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccagggt
    17341 agcgtcttca acgcggtcac gggatgcttc agaagcacgc aggtaacctt caacgccgtc
    17401 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
    17461 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
    17521 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
    17581 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
    17641 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
    17701 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
    17761 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
    17821 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
    17881 ccaatccatt tcggaaacgt gtaccaggcc ttcaacgcct tcttcgattt caacgaagca
    17941 gccgtagtcg gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
    18001 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
    18061 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
    18121 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
    18181 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
    18241 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
    18301 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt tgaattcaag
    18361 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
    18421 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
    18481 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
    18541 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
    18601 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
    18661 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
    18721 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
    18781 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
    18841 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
    18901 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
    18961 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
    19021 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctca
    19081 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
    19141 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
    19201 accgttccca tgtcacggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
    19261 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
    19321 acttcctgag tacgaatttc gccactgaca tcttcccctt cgaggatcac ttccagattg
    19381 ccattggtcg acacaaaacg tacatccaga tgggatgcca gcggcaccag cgcatcttcc
    19441 gatgcaacat caacatggtg atgtaatgcc gccagtgcca gtacgcgata aattgcaccc
    19501 gagtccagca gatgccattg caacgcttcc gccatagcct tacacaaggt gcctttccct
    19561 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
    19621 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
    19681 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
    19741 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
    19801 atcacggatg tgttgcgcac gctctgttga tggtgggtga gaatcaaaca tggactgggt
    19861 acgaccgcca tccaggctgg ccaatttttc aaagctgcca accagcccct gggtgctgat
    19921 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcgcggga
    19981 gtatttagca ttgatcgcgc cttctgcgat atcacctaat tgtgagcgag aaagctggga
    20041 agccacaccg ctggtagcgg aaatcgcatc acgtgcggca acgatcgcat aagaagcttt
    20101 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatgaccca gaacgccttc
    20161 aatttcgttg tcgttcatca tgtccatcag gccactgtag acacgaacac aaccgttcgc
    20221 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
    20281 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttcccgtact tgctggattt
    20341 gcttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
    20401 atcagcatcg cttaacgttg ctgctttata ggcatttaaa cctgagctga tagccatatt
    20461 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg ccgcaatcgc
    20521 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
    20581 tgtatcaata acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
    20641 cgaaaaaaaa cgccggcaat gtgccgacgt tttgataaag aaagatttgg ctatttattg
    20701 cacgttgttc attcaggctg cctggctaat ccgcgccagc tgctcgaaat aattcggaaa
    20761 tgttttggcc gtgcatttgg gatcaagaat cgtcactggt gtatctgaca acgccaccag
    20821 cgagaaacac atcgctatcc ggtgatcatt gtatgtcgcg atctcggcaa agttcagctt
    20881 ttccggtgga gtgatacgaa tgtaatcgtg cccctcttct acttccgcac caactttacg
    20941 cagttctgtt gccatcgcaa acaggcgatc ggtctcttta acacgccagt tatagatatt
    21001 gcgcagcgtg gtggtgcctt ttgcaaataa cgccgccgtg gcaatggtca tcgccgcatc
    21061 gggaatatgg ttcatatcca tatcaatagc gttcagttca ccccgcgtgc aggaaatata
    21121 atcatcgccc cagcaaatgg tcgcgcccat tttttccagc acatcagcaa agcgaatatc
    21181 accctgcata ctgttgcgtc caatgccggt cacttttaca gtgccgcctt tgattgctgc
    21241 tgctgccagg aagtaagaag ccgaagatgc atcgccttcg accaaataag tacccggaga
    21301 ctgataagac tgcccgcctt ttacgacaaa ttgttgatag tgctgatttt caatttcaac
    21361 accaaacgtc ttcatcatat tgagtgtgat gtcgatataa ggtttagaaa ccagatcgcc
    21421 tttaatacga atcaccgtat cttccggtgc aagaggcgca gtcattaata gtgcggtgag
    21481 gaactggctg gaaacggagc catcaacgtc aacgttgccg ccagtaaagc cgccctgtaa
    21541 acgcaatggc ggataattgt cttgttccag gtaagtgatc ttcgccccgc ccaggcgcag
    21601 cgcatccacc agatgaccaa tcgggcgctc tttcatacgc ggctcaccag tcagcacaat
    21661 atcattgcta cccagacaaa gagctgccgc cagcgggcgc attgccgttc cggcgtttcc
    21721 gaggaacaac tccagggcac cttctgcgtg taatggaccg ccgttaccga taatttcgca
    21781 acgcgtacga tcggctgaaa gcgtatagct tacccctaac gctgttaatg cattcagcat
    21841 atggcgcacg tcatcgctat ccagcagatt ggttaatact gttttgccgt gtgctaatgc
    21901 cgccagcaat aaagcgcggt tagaaacgct cttggaacca ggcagattaa tagtgccatc
    21961 gacacgagcg atgggttgta acgtcaggga ttccatgaac tcaactctct acaacagaaa
    22021 taaaaacccc acaggctggc tgtggggatt aagcaaaatt tcggcattag ccgtgacggc
    22081 gttcgaactc aaccatgaag tctgtcaggg ctttaacgcc ttccagcggc atggcgttat
    22141 aaatagacgc gcgcattccg ccgaccacac ggtgaccttt cagcgcatga aggccagcag
    22201 caaaagactc ttcaaggaac aatttgtcaa gcgcactgtc cgccaactgg aacggcacgt
    22261 tcatccgcga acggttagct ttcgccacgt cattgcggta gaaattgctg ttatcaatca
    22321 ccccatacag cagttctgct ttttgctgat tgattttatc cattgcagct acaccgccgt
    22381 tcgctttcag ccatttaaag accagaccag acagatacca ggcaaatgtc ggcggcgtgt
    22441 taaacatgga gtcgttatcg ttgagaatgg aataatccag aatcgacgga cacgcgatat
    22501 tcgctttgcc cagcaaatct tcacgaacga tgacgattgt caggccagcc gggccgatat
    22561 ttttctgcgc gccagcgtaa atcacgccat aacggctgac gtcaatcgga cgggaaagaa
    22621 tggttgaaga gaagtcggcg gcgaccacca catctttgcc gaagtctggc gtttcgtcga
    22681 tagcgatacc gtcgatggtt tcattcgggc agtaatgcat ataagcagca ttatcagaga
    22741 gttgccattc gcgcattggc ttaaccgcgc gcagaccatc aacagtcact ttggcgtcaa
    22801 agacattagg cgtgcagtat tttttcgctt ctttaatggc acttgccgcc cagtaaccgg
    22861 catcaacata atctgcggtg gttttatcac cgagaatatt cagcggaaca gcagcaaact
    22921 gaccgcgacc gccgccatgg cagaacaata ccttgtagtt ggaggggaca ttaagaagat
    22981 cgcgaaaatc cttctcggct tcctctgcaa cctgaatgaa ctctttgcca cggtgactca
    23041 cttccatcac cgacgtacca agaccgttcc agtcgcgcag ttcctgttga gcctgtttaa
    23101 gcacctctgc cggtagcatt gccggaccag aactaaaatt gaagatttga gccatttccc
    23161 ctcaccacgt tgcgttgcta ttaacccgcg gtcaatcgat tgcgaccaca ggtaatacat
    23221 tattcctgtg gctatcggtt ttatcattca gtgacacaca tcgcaatgag taaaaccgtt
    23281 gagggcaaaa atgtggcttg catcacacaa aagaatctct cggattatta gcaaaaagcc
    23341 gatgttttta ctgtcacgtc acccgttttg cccgacttgc cttcgtccat tctggatttg
    23401 cacagcgcgg acataattgt ttttcccccg cgttcatatg aatgatttca ctgcaacgaa
    23461 tgcaggcata gtccaccgac tcgggtacca ctttgtaacg ctgagtacag tcactgggta
    23521 aaataagtaa gcccggtttc accttgtcat cttcaaagaa atagacactg atttgcccgt
    23581 tggtttcgag aatcgccaga cgtacttgcc ccaactgctc tacaccgcgc aatcgtagct
    23641 ccataaagaa ctcaaattcc gtcatgttgg agttattgag tttcgaccag gccagctcgc
    23701 catcttcaat aatgacaacc ggcttgcctt ccagaagatc ttccagtttt tcactgtgcg
    23761 ccatcaacca cattaccagg cggtataaca acgccagaat aataaagacg ataagtaccg
    23821 ggaccatcgg cacatcatca taaaacgcca catctcccgc cgctgatccc agcgtcagaa
    23881 tgattaacac ttcaaaaagc gacatctgcc gcacaccgcg tcttccggtc attttgagga
    23941 acaaaaaaac cagtacaaag gtataaagac tacgtagtgc cacttcgcca aggaaatcaa
    24001 aaggcacttt atcaaatgcc atacggtgga gatcgaacgc cttcatttat atgctcttat
    24061 cgtggtttat ccgctaaaag catagtcaac agatcatcgg catggcggcg gtgatagcgc
    24121 ctttgagata aaacccgtat tattgcgcgc tttccgtacg actaaagtga ttttcatgac
    24181 gcaaacattt atccccggca aagatgccgc actggaagat tccatcgctc gcttccagca
    24241 aaaactttca gacctcggct ttcagattga agaggcctcc tggctgaatc cggtgcctaa
    24301 cgtctggtct gtacatattc gcgacaaaga gtgcgcactg tgttttacca acggtaaagg
    24361 cgcaaccaaa aaagcggcgc tggcttctgc actcggtgaa tatttcgagc gtctctcaac
    24421 caactacttt tttgctgatt tctggctggg cgaaaccatc gccaacggtc cgttcgtgca
    24481 ttatcccaac gaaaaatggt tcccactgag cgaaaatgac gatgtgccag aagggctgct
    24541 cgatgaccgt ctgcgcgcat tttacgatcc ggagaatgaa ctggccggca gtatgctgat
    24601 tgacctgcaa tccggtaacg aagatcgtgg tatttgcggt ctgccgttta cgcgtcagtc
    24661 tgataatcag accgtttata ttccgatgaa tatcattggt aacctgtatg tttctaacgg
    24721 tatgtccgct ggcaataccc gtaacgaagc acgcgttcag gggttgtccg aagttttcga
    24781 acgctacgtg aaaaaccgca ttattgctga aagcatcagc ttgccggaaa tcccggcaga
    24841 cgtgctggcg cgttacccag cggtggttga agccatcgaa acgctggaag cagaaggttt
    24901 cccaatcttt gcttatgatg gttcgcttgg cggccagtat ccggtgattt gcgtggtact
    24961 gttcaatcct gctaacggta cctgctttgc ctctttcggt gcgcatcctg attttggcgt
    25021 agcactggaa cgtaccgtga ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt
    25081 gtttactccg ccaaccttcg atgatgaaga agttgctgaa cataccaacc tcgaaacgca
    25141 ctttatcgat tccagcggtt taatctcctg ggacctgttc aagcaggatg ccgattatcc
    25201 gtttgtggac tggaatttct ccggcactac ggaagaagag tttgctacgc tgatggctat
    25261 cttcaacaaa gaagataaag aagtttatat tgccgattac gagcatctgg gcgtttatgc
    25321 ttgccgaatt atcgtgcctg gcatgtccga tatttatccg gctgaagatc tgtggctggc
    25381 gaataacagt atgggcagcc atttacgtga aacgattctt tcgttaccag gcagcgagtg
    25441 ggaaaaagaa gattacctga acctcatcga gcaactggat gaagaaggtt ttgatgactt
    25501 tacccgcgtg cgtgagctgt tgggtctggc gaccgggtcg gataacggtt ggtacaccct
    25561 gcgtatcggt gaattaaaag ccatgctggc gctggctggt ggcgatctgg aacaggctct
    25621 ggtctggacc gaatggacga tggagtttaa ctcatcggta ttcagcccgg aacgcgccaa
    25681 ctattatcgc tgcctgcaaa cgttgttatt actggcgcag gaagaagatc gccagccgct
    25741 gcaatatctg aatgcgtttg ttcgcatgta cggcgcagat gccgtggaag ccgccagtgc
    25801 ggcaatgagc ggcgaagcgg cgttttatgg tttgcaacca gtagatagcg atctgcacgc
    25861 gtttgctgca catcagtcgt tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc
    25921 attctgggca aaataaaatc aaatagccta cgcaatgtag gcttaatgat tcgtctgagt
    25981 tatattacgg ggcgtttt
//
LOCUS       DAZGAP010000065        25918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGAP010000065 DAZGAP010000000
VERSION     DAZGAP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.065"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..980
                     /locus_tag="ACHLAM_002228"
                     /pseudo
     CDS             17..980
                     /locus_tag="ACHLAM_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            1149..1754
                     /gene="ynbA"
                     /locus_tag="ACHLAM_002229"
     CDS             1149..1754
                     /gene="ynbA"
                     /locus_tag="ACHLAM_002229"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7853749.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            1754..2650
                     /gene="ynbB"
                     /locus_tag="ACHLAM_002230"
     CDS             1754..2650
                     /gene="ynbB"
                     /locus_tag="ACHLAM_002230"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7853750.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWVTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGLNC
                     FELFLLFIPLFGFLILATWRVFVGEPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            2666..4423
                     /gene="ynbC"
                     /locus_tag="ACHLAM_002231"
     CDS             2666..4423
                     /gene="ynbC"
                     /locus_tag="ACHLAM_002231"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO7853751.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALVFEKMQ
                     SFISHLYANKSQKLDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRGN"
     gene            4437..5729
                     /gene="ynbD"
                     /locus_tag="ACHLAM_002232"
                     /pseudo
     CDS             4437..5729
                     /gene="ynbD"
                     /locus_tag="ACHLAM_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(5783..6388)
                     /gene="azoR"
                     /locus_tag="ACHLAM_002233"
     CDS             complement(5783..6388)
                     /gene="azoR"
                     /locus_tag="ACHLAM_002233"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO7853752.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            6589..10491
                     /gene="hrpA"
                     /locus_tag="ACHLAM_002234"
     CDS             6589..10491
                     /gene="hrpA"
                     /locus_tag="ACHLAM_002234"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO7853753.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELSLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            10763..11563
                     /gene="ydcF"
                     /locus_tag="ACHLAM_002235"
     CDS             10763..11563
                     /gene="ydcF"
                     /locus_tag="ACHLAM_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO7853754.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            11760..13199
                     /gene="aldA"
                     /locus_tag="ACHLAM_002236"
     CDS             11760..13199
                     /gene="aldA"
                     /locus_tag="ACHLAM_002236"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO7853755.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(13240..14241)
                     /gene="gap"
                     /locus_tag="ACHLAM_002237"
     CDS             complement(13240..14241)
                     /gene="gap"
                     /locus_tag="ACHLAM_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7853756.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            14430..14960
                     /gene="cybB"
                     /locus_tag="ACHLAM_002238"
     CDS             14430..14960
                     /gene="cybB"
                     /locus_tag="ACHLAM_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO7853757.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            15205..15378
                     /locus_tag="ACHLAM_002239"
     CDS             15205..15378
                     /locus_tag="ACHLAM_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853758.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
     gene            15404..15472
                     /gene="yncP"
                     /locus_tag="ACHLAM_002240"
     CDS             15404..15472
                     /gene="yncP"
                     /locus_tag="ACHLAM_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO7853759.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(15450..15599)
                     /gene="hokB"
                     /locus_tag="ACHLAM_002241"
     CDS             complement(15450..15599)
                     /gene="hokB"
                     /locus_tag="ACHLAM_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO7853760.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            15998..17638
                     /gene="trg"
                     /locus_tag="ACHLAM_002242"
     CDS             15998..17638
                     /gene="trg"
                     /locus_tag="ACHLAM_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO7853761.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(17676..18599)
                     /gene="ydcI"
                     /locus_tag="ACHLAM_002243"
     CDS             complement(17676..18599)
                     /gene="ydcI"
                     /locus_tag="ACHLAM_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7853762.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            18816..20159
                     /gene="ydcJ"
                     /locus_tag="ACHLAM_002244"
     CDS             18816..20159
                     /gene="ydcJ"
                     /locus_tag="ACHLAM_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7853763.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            20384..22039
                     /gene="opgD"
                     /locus_tag="ACHLAM_002245"
                     /gene_synonym="mdoD"
     CDS             20384..22039
                     /gene="opgD"
                     /locus_tag="ACHLAM_002245"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO7853764.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            22179..22403
                     /gene="ydcH"
                     /locus_tag="ACHLAM_002246"
     CDS             22179..22403
                     /gene="ydcH"
                     /locus_tag="ACHLAM_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO7853765.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            22466..23005
                     /gene="rimL"
                     /locus_tag="ACHLAM_002247"
     CDS             22466..23005
                     /gene="rimL"
                     /locus_tag="ACHLAM_002247"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO7853766.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(22997..23977)
                     /gene="ydcK"
                     /locus_tag="ACHLAM_002248"
     CDS             complement(22997..23977)
                     /gene="ydcK"
                     /locus_tag="ACHLAM_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO7853767.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDCETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            24101..25093
                     /gene="tehA"
                     /locus_tag="ACHLAM_002249"
     CDS             24101..25093
                     /gene="tehA"
                     /locus_tag="ACHLAM_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO7853768.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
     gene            25090..25683
                     /gene="tehB"
                     /locus_tag="ACHLAM_002250"
     CDS             25090..25683
                     /gene="tehB"
                     /locus_tag="ACHLAM_002250"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO7853769.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         6458 a         5977 c         6723 g         6760 t
ORIGIN      
        1 taacgtaacg taacgtaacg taacggcaac gaatatagtt acggattcac cactgttaat
       61 taccggttcg ataaatgcaa gtggactggt atttatcgac tcatattacg ataatccgtc
      121 tacgattaag ggaagtatta atgcgcgcgg gatatttatc aatgacataa ttgcacctgt
      181 tgttgcatct tcgacaaata gtgaattcat ggtccgtgcg agtgacaaaa atgacactga
      241 aaatgtcaaa aaagcgctga tgataataaa tcctgatgca tattattggg gcctaataaa
      301 tgatgaagat gctctgaaag aaatttttaa gcgaagcaat attcgcatag cagggaatgt
      361 ctgtaatcag atgaaaaaag aagcgctgtt tcgccctaag tcttctcctg aattattgca
      421 ggaattgcaa atgctggatg aaggcaacgt tgctgcattt gaaggacgag acattgcgac
      481 atttgatctt gccataatgc gaactctccc aaggcttaaa ggaattacag caaaacacgc
      541 cacgaaatga tatttgggcg aagttttttc tgaggcagga aaacagttcc cgtgcgcagg
      601 ttaacgaagc gttacgtgtt tattatgcac tagaacccga tgcgttagca caactggatg
      661 tactggcaaa acaaccggat cgtatatggt ggagtacact ggcgaaaagc aatctcacat
      721 ttttcaagtt cggcgcactt aacaaccgcc acacaccgcc tgcagtactg gcggcagaaa
      781 ttgatcccga gtggtggatt gtggcgatga ataatccccg ttttccagtt gatgtattaa
      841 aggcgaggct gaaacgtgat cctttgctgg cgttagagct tgttaaccct gaactggatt
      901 tagtccgcca gctggcgctt aacggtaaga cgcgcgcaat acgggaacaa gcgatgagaa
      961 aacttgatga gttgcattga ataacagcgt aatattttta actatttgat taattgggga
     1021 taatcattcc tgacagtgag ccccaatacc ttgatatatt ctgaattttt aatgaagcgg
     1081 cgtgttgcga tatctctgcc agggcaattg atgcaccata gcgcaaacag aattatcaag
     1141 gattgataat gacgctctac cagataaaac cgctctttca gtcactgtta aggccgacga
     1201 tgttttggct tcataagcgg caagtgtctg caaatcagat cacccttgcc gcgttggcac
     1261 tttctcttct caccggtttg ttgttggcgt tggtgccata tcatggcatg tttctgctat
     1321 tgcccatcgt gctttttgtc cgtatggcac tcaatgcgct ggatggcatg atggcgcgtg
     1381 agtgcaatca gcaaacacgt cttggggcga ttttgaatga aactggcgat gttatttccg
     1441 atattgcgct ttatttaccg tttttatttt taccggaaag taacgcatca ctcgtaatac
     1501 tcatgttatt ttgcaccata ttgactgagt tttgcggttt actcgcgcag acgattaacg
     1561 gtattcgcag ttatgccggt ccatttggca aaagcgatcg cgcgttaata tttgggttgt
     1621 gggtgctggc cgttgccatt tatccacagt ggatgcaatg gaataatctt ttatggagca
     1681 ttgcttcaat tctgcttctc tggactgcga ttaatcgttg tcggagtgtg ctcctgatga
     1741 acgctgaaaa ataatgctgg aaaaatctct ggcaacactt ttcgcgttgt taattttagc
     1801 aacgctgata aaccgttttc tggtatggcg gttaccggag agaaaagggg atgaagttac
     1861 attacgtatt cgtacctggt ggggcatcgt catttgtttt tcactggtga tttcaggtcc
     1921 acgctgggtg acgttgacgt tttttgcgct gataagtttt ctggcattga aagaatattg
     1981 tacgcttata tccgtacatt ttccgcgttg gttatattgg gttattcctc ttaactattt
     2041 gcttatcggt ttaaattgct ttgagctgtt tctgttattt atacctttgt ttgggtttct
     2101 gatattagcc acctggcgag tttttgtggg tgaaccctcc ggttttctgc ataccgtgag
     2161 cgccattttt tgtggctgga taatgaccgt tttcgccttg agtcatgccg cctggttatt
     2221 aatgttgcca accacaaata tccagggcga ggcgttactg gtgttatttc ttttggcatt
     2281 aaccgagtca aacgatattg cacagtattt atgggggaaa tcctgcggca gaagaaaagt
     2341 ggtccccaaa gtcagcccgg gaaaaacgct ggaaggtctg gtggggggcg taatcactac
     2401 catgattgtg tcactgatta ttggaccgtt actgacaccg ctagatacat tacaggcatt
     2461 attagcgggt ttgttaattg gtattagtgg tttttgcggc gatgtagtga tgtcagccat
     2521 caaacgagat gttggtgtta aagatagtgg aaaactatta ccaggacatg gcggactcct
     2581 tgacaggatt gactcattaa ttttcaccgc tccggtattt ttttatttta ttcgctattg
     2641 ctgttactga aggaaaatag agagaatgga aaattcacgc atccctgggg aacatttttt
     2701 taccaccagt gataatacag cgttgtttta tcggcactgg cccactttac agccaggggc
     2761 gaaaaaggtc atcgtcttat ttcatcgcgg tcatgaacat tctggtcgtc tacaacatct
     2821 cgttgatgaa ctggcgatgc cagatactgc tttttatgca tgggatgccc gagggcatgg
     2881 acaaacttcg gggccgcgtg gttatagtcc gtctcttgcg agttcagtgc aagatgtcga
     2941 tgaatttgtc cgttttgctg ccagcgacag ccaggtcgga ctggaagagg tggttgtgat
     3001 cgcgcaaagc gtcggcgcag tgatggttgc tacatgggtt catgattatg cgcctgctat
     3061 tcgcgggctg gtgctggctt ctccggcctt taaggttaaa ttgtatgtgc cgctggcacg
     3121 tcctgcgctg gcgttatggc atcgtctgcg tggtctgttt tttattaatt cctatgtgaa
     3181 aggacgctat ttgacccacg atcggcaacg ggtggctagt ttcaataatg atccgctgat
     3241 cacaagggcg attgccgtca atatcttgct agatctctat aaaacgtctg aacgtattat
     3301 tagcgatgcg gcggcgatta cgctccccac gcaacttctg atatcaggcg atgactatgt
     3361 ggtgcaccgc caaccgcaga ttgattttta tcagcgttta cgtagccctc tgaaagagct
     3421 gcatctgctg ccaggctttt atcacgacac gttgggtgaa gagaacaggg cgctggtatt
     3481 tgaaaaaatg caaagcttta ttagtcactt atatgctaac aaatcgcaaa aattagatta
     3541 tcagcatgaa gactgcacag gaccatcagc ggatcgatgg cggctacttt ctggtggacc
     3601 cgtgccatta tcgccggttg atttggcgta tcgctttatg cgtaaagcga tgaaattgtt
     3661 cggggcgcac tctgcgggcc tgcatctcgg aatgagcacc ggctttgatt caggcagttc
     3721 gctggattat gtctatcaaa atcaaccgca aggtagtaac gcattcgggc gtttagtcga
     3781 caaaatctac ctgaacagtg ttggctggcg cggtattcgc cagcgcaaaa cccatttaca
     3841 aatgctgatt aaacaagccg ttgccgatct ccacgctaaa ggtttagccg tccgcgtggt
     3901 tgacattgcc gcagggcatg gacgctatgt actggatgcg ctggcgaacg agcctgccgt
     3961 aagcgatatt ttcttacgtg attacagtga gttaaatgtt gcacaggggc aagagatgat
     4021 tgcccaacgg ggaatgtctg ggcgggtgcg ttttgaacag ggcgatgcgt ttaacccgga
     4081 ggagctcagc gcgttaactc cgcggcctac gctggcgatt gtctctggac tgtatgagct
     4141 ttttcccgaa aatgagcagg taaaaaactc actcgcaggt cttgccaatg ccatcgatcc
     4201 gggtggcatt ctcatctaca ccgggcagcc gtggcaccca caactggagc tgattgccgg
     4261 ggtgttaacc agtcataaag atggtaaacc gtgggtaatg cgcgtgcgtt ctcaagggga
     4321 gatggattcg ctcgtgcatg atgccggatt cgataaatgc acacaacgga ttgatgagtg
     4381 gggcattttt acggtttcga tggcggtgcg tcgtggtaac tgaacgccgg aacgtattgc
     4441 tacaaggcgc tggctggtta ttgttgctgg ccccgttttt cttcttcaca tatggatttc
     4501 ttaatcagtt caccgcgact caggatctta acaaccatga tatccccagt cagttattcg
     4561 gttgggaaac ggcgatccct tttcttccct ggactattgt tccttactgg agtctggatc
     4621 ttttatatgg attttcgctg tttgtttgta gctcgacatt tgagcagcgc cgtctggtcc
     4681 accggcttat tctggcaacg gtaatggcct gctgcggttt ttttctctat ccgctgaagt
     4741 ttagttttat ccgtcctgaa gtgagtgggg tgacaggatg gttattttcg caacttgaat
     4801 tgtttgatct gccttataac cagtctcctt cgctgcatat tattctctgc tggctacttt
     4861 ggcgtcactt tcgtcagcat ctggctgtga ggtggcgtaa agtctgcggc ggatggtttt
     4921 tactcatcgc catttcgacg ctgacgacct ggcagcatca ttttattgat gtcatcacag
     4981 ggctggcggt aggtatgtta attgactgga tggtgcccgt cgaccgtcgt tggaattatc
     5041 agaaacctga tcaacgtcga atcaaaatag cactgccata tgtcgtaggc gcgtgctcgt
     5101 gcattgtgtt gatggagcta atgataatgc ttcagttatg gtggtcagtc tggttatgtt
     5161 ggccagtatt atcgctattc atcattggcc gtgggtacgg tgggcttggc gcgataacaa
     5221 cagggaaaga tagttagggg aaactcccgc ccgccgttta ctggctgaca ttgccctggc
     5281 gcatcgggat gtggctgtct atgcgttggt tttgtcttcg cctggagtcg gtgagcaaaa
     5341 ttactgctgg tgtttattta ggggcgtttc cacaacatat tccggcacag aatgctgttc
     5401 tggacgtcac ctttgaattc cctcgcggac gagccacaaa agatcaactc tatttttgtg
     5461 taccgatgct ggatctggtg gttccggaag agggggagct ccgacaggcc gtggcgatgc
     5521 tggaaacatt acgcgaagag caaggcagcg ttctggtcca ttgcgcgttg ggattatcgc
     5581 gcagtgcgct ggtggtggcg gcatggctgt tatgttacgg acattgtaaa accgttgatg
     5641 aagcgattag ttatattcga gccagacgct cgcggattgt gcttaaggaa gagcacaaag
     5701 cgatgctgaa attatgggaa aacaggtaag cggattgaga tgtggactga atatctacag
     5761 tccacatcaa gatcgtgtcc agttattcag caacaatact gtcgatggct gctttcgcgt
     5821 cagactgcgc tttcgctgcc atttcaggac cgtatgcgat cccttcggcg aagacaaatt
     5881 tcacatcggt aatgccgata aagcccagga atgtggacag atacggcgtc accagatccg
     5941 ttgggccatc tttatggatc ccaccgcggc tggtgataac gatggctttt ttaccggtga
     6001 ccaggccttc cggaccgttc tccgtatagc ggaaagtaac gcctgcgcgt gcaaccaggt
     6061 caaaatagtt tttcaactga gtcgagatgt tgaagttata catcggtgcc gcaataacga
     6121 taacgtcgtg ggctttcaac tcggcaatca gctcatcgga aagtgccaga gcttcctgct
     6181 gacgcggagt cagcggtgca tcgctcggac gcagcgcacc aaccagttcg ccatccagca
     6241 ccggaatcgg atttgcagcc aggtcgcgaa cggtgatttc atcagcggag tgcttttcgc
     6301 gccattgttc aacaaaataa tcggacaact gattagactg agagtaccct gccaggatgc
     6361 tggatttaag aactaatacc ttgctcatgg tgtttcctta tagatgtttg aatgggcgat
     6421 gccccgttgc ttgttgacac tttattcaca atcctgccac agagatagcg caataaatcg
     6481 aagcctatgt tcgaatttat tgaacaacgc atagaaagcc gcgatgtggt actctatatc
     6541 tatcatttaa aagaaaatta atcaggcaga ctactgccca ctaacgttat gacagaacaa
     6601 caaaaattga cctttacggc cttgcagcag cggctggatt cgctgatgct gcgcgacaga
     6661 ctgcgttttt ctcgccgtct gcacggcgtg aagaaggtta aaaatcctga tgcacaacag
     6721 gccattttcc aggagatggc gaaagagatt gaccatgcgg cagggaaagt cctgctgcgt
     6781 gaagcggcac gaccggaaat cacttatccg gacaatttgc cggttagtca gaaaaagcag
     6841 gacatactcg aagcgattcg tgatcaccag gtggtgatcg tcgccgggga aaccggttcc
     6901 ggtaaaacga ctcagctacc gaaaatctgt atggaactgg ggcgcgggat taaagggctg
     6961 atcggtcata cccaaccgcg tcgcctggcg gcacgaacgg tggcgaaccg tattgcggaa
     7021 gagctgaaaa cggagccggg cggttgcatc ggttacaaag tgcgtttcag tgatcacgtt
     7081 agtgataaca cgatggtcaa gctgatgacc gacggtatcc tgctggcgga gatccagcaa
     7141 gaccgcctgc tgatgcagta cgacactatc attattgacg aagcgcacga acgtagcctg
     7201 aatatcgatt ttctgctcgg ctatttgaaa gagttgctac cgcggcgtcc tgacctaaaa
     7261 atcattatca cttccgcgac tatcgacccg gaacgctttt cgcgccactt taataatgcg
     7321 ccgattatcg aagtctccgg tcggacctat ccggtggaag tgcgctatcg accgattgtt
     7381 gaagaagccg atgacaccga gcgcgatcag ctgcaggcga tttttgacgc cgtagacgaa
     7441 ctgagccagg aaagccctgg cgacattctg atctttatga gtggtgagcg ggaaattcgc
     7501 gataccgccg atgcgctgaa caagctgaac ttacgccata ccgaaatctt gccgctttat
     7561 gcgcggcttt cgaacagcga acagaaccgc gtgttccagt cgcacagtgg acggcgcatt
     7621 gtgctggcga ccaacgtcgc ggaaacgtcg ctgaccgtac cgggtattaa atacgttatc
     7681 gacccaggta cggcgcgtat cagccgctac agctatcgca ctaaagtgca gcgtttgccg
     7741 attgagccga tttctcaggc gtctgccaat cagcgtaaag gccgctgtgg tcgtgtgtcc
     7801 gaagggatct gtattcgtct ctattccgaa gacgatttcc tctcgcgccc ggagtttacc
     7861 gatccggaga ttctgcgtac taacctggcc tcggttattt tgcagatgac cgcgctgggg
     7921 ttgggcgata tcgccgcgtt cccatttgtc gaagcaccgg ataaacgtaa tatccaggat
     7981 ggcgtgcgtc tgctcgaaga gctgggcgcg atcaccactg atgaacaggc cagcgcctat
     8041 aaactgacgc cgctcggtcg ccagctttcg caattgcctg tcgatcctcg tctggcgcgt
     8101 atggtgctgg aagcgcaaaa acatggctgc gtgcgtgagg cgatgattat cacgtccgcg
     8161 ctctccattc aggatccgcg cgagcgtccg atggataaac agcaggcatc agacgaaaaa
     8221 catcgccgct tccacgacaa agaatccgac ttcctcgcgt ttgtaaatct gtggaattat
     8281 ctcggcgagc agcaaaaggc gctttcttct aacgccttcc gtcgcctgtg tcgtaccgat
     8341 tatctcaact atctgcgcgt gcgcgaatgg caggatatct acacccagtt gcgccaggtg
     8401 gtgaaagaac ttggcattcc ggttaacagc gaaccggcgg agtatcgcga aattcacatt
     8461 gcgttgctga cgggtttgct ttcccatatc ggcatgaaag atgccgataa acaagaatat
     8521 accggcgcac gtaacgcgcg tttttccatc tttcccggtt ctggtttatt caaaaagccg
     8581 ccgaaatggg taatggtggc ggaactggta gaaaccagcc gcctgtgggg gcgcattgct
     8641 gcgcgtatcg accctgaatg ggtagaaccc gttgctcagc atttgattaa acgcacctac
     8701 agcgaaccgc actgggaacg ggcgcagggc gcggtgatgg caacggaaaa agtcactgtt
     8761 tatggtttgc cgattgttgc tgcgcgtaag gtcaactaca gccagatcga tccggcgtta
     8821 tgtcgtgaac tctttattcg ccacgctctg gtggaaggtg actggcagac gcgtcacgca
     8881 ttcttccgtg aaaacctgaa actgcgggcc gaagtggaag agctggaaca caaatcacgt
     8941 cgccgcgata ttctggttga tgacgaaacg ctgtttgagt tctacgacca gcgcatcagc
     9001 cacgatgtaa tctccgctcg tcacttcgat agctggtgga aaaaagtcag ccgcgaaacg
     9061 cctgatttgc tcaactttga aaagagcatg ttgatcaaag aaggggcgga aaaaatcagc
     9121 aagctggatt atccgaactt ctggcatcag ggcaatctca agctgcgttt gagctatcag
     9181 tttgagccag gcgcggacgc ggacggtgtg accgtgcata ttccgctgcc gctgcttaat
     9241 caggtagaag agagcgggtt tgaatggcaa atccccggtc tgcgccgcga actggtgatt
     9301 gctctgatta aatcgttgcc gaaaccagta cgccgtaatt ttgtgcccgc gccaaactac
     9361 gccgaagcgt ttctaggccg cgtcacaccg ttggaattgt cgttgctcga cagtcttgaa
     9421 cgagagctac ggcggatgac tggcgtcacc gttgaccgcg aagactggca ctgggatcag
     9481 gtgcccgatc acctgaaaat caccttccgt gtggtggatg acaaaaacaa gaagctaaaa
     9541 gaagggcgct cattacagga tctgaaagat gctctgaaag gcaaagtgca ggaaacgctg
     9601 tctgcggtgg cggatgacgg tatcgagcag agcggcttac atatctggag ctttgggcaa
     9661 ctgccggaaa gctacgaaca gaagcgtggc aactacaaag tgaaggcgtg gccagcgttg
     9721 gtggatgagc gcgacagcgt ggcgatcaaa ctgtttgata acccgctgga acaaaagcaa
     9781 gcgatgtgga acggtcttcg tcgtttactg ctgctgaata ttccatcgcc gatcaagtat
     9841 ttgcatgaaa agttaccgaa caaagccaag ctggggctgt actttaaccc gtatggcaaa
     9901 gtgctggagc tgatcgacga ctgtatctcc tgcggggtgg atcaactgat cgacgccaat
     9961 ggaggcccgg tctggacgga agaaggcttt gctgcgctgc atgagaaagt ccgcgctgaa
    10021 ctgaacgata cggtggtgga tattgcgaag caggtcgagc aaatccttac ggcagtgttc
    10081 aatatcaaca aacgcctgaa agggcgggtg gatatgacta tggcgctggg gctttctgac
    10141 atcaaagcgc agatgggcgg gctggtgtat cgcggttttg tcactggtaa cggttttaaa
    10201 cggctgggcg acacgctgcg ttatttgcag gcgattgaaa aacgactgga aaaactggcg
    10261 gttgatccgc atcgtgaccg tgctcagatg ctgaaagtcg aaaatgtcca gcaggcgtgg
    10321 cagcaatgga tcaacaaact gccgcccgca cgtcgtgagg atgaagacgt gaaagagatc
    10381 cgttggatga tagaagagtt gcgcgttagt tacttcgctc aacaacttgg tacgccttat
    10441 ccgatttcag ataagcgtat tttgcaggcg atggaacaga ttagcggtta acgcggctat
    10501 ttgcctgata aatctaaaac ccggtaagca tttagcgccg ggttttttat gaattctaaa
    10561 acggtaaggg caaaaattca ggaattcaga aaaatacaat tctcagctgc aagatgaata
    10621 atgtttatct acagcatttc cttaaaagat atgtcaggct tgcggagtgg cggttaagga
    10681 aatacgactt cctcctttca gagtgctccg cttctcacta ttatctcacg cagtattctt
    10741 aagggaacga taaggaggaa ccatgaacat taccccgttt ccgacgcttt cgccggcaac
    10801 catagatgcc ataaatgtta tcggtcagtg gctggcgcag gatgatttct ccggtgagat
    10861 gccgtatcag gccgattgcg tgatccttgc aggcaatgcg gttatgccga ctatcgacgc
    10921 ggcatgtaag attgctcgcg atcagcaaat tcctctactg attagtggtg gtatcggtca
    10981 ctcgacaact tttttgtata gcgccatcgc acagcatccg cactacaaca ctatccgcac
    11041 cactggcaga gcagaagcga ccatcctggc ggatatcgct catcagttct ggcacattcc
    11101 gcatgaaaaa atctggattg aagaccagtc aacaaactgc ggtgaaaacg cacgctttag
    11161 catcgcgcta ttgaatcagg ccgtagaacg agttcatacg gctatcgttg ttcaggaccc
    11221 caccatgcag cggcgcacga tggcgacgtt ccgccgtatg actggggaca atcccgatgc
    11281 accacgctgg ttaagttatc ccggattcgt tcctcagtta ggaaataacg cagacagtgt
    11341 aatctttgtt aatccgttgc aaggattatg gccggttgag cgttatctct cactactcac
    11401 tggcgagctg ccgcgtttac gcgatgatag cgatggctac ggtccccgcg ggcgagattt
    11461 tatcgttcac gttgattttc cggcagaagt catccatgca tggcaaacgc tgaaacacga
    11521 tgcggtgcta atcgaggcga tggaaagtcg ctcgttacgt taaaaattgc ccgtttgtga
    11581 accacttgtt tgcaaacggg cataactcct gccttttatt tctgcctctt attcctttta
    11641 tacttgtttt tatgaagccc ttcacagaat tgtcctttca tgatgccgtc tctatgatga
    11701 ttgatgttaa ttaacaatac atttaccaaa aacaaacata taaatcacag gagtcgctca
    11761 tgtcagtacc cgttcaacat cctatgtata ttgatggaca gtttgttacc tggcgtggag
    11821 acgcatggat tgatgtggta aaccctgcta cagaggctgt catttcccgc attcccgatg
    11881 gtcaggccga ggatgcccgt aaggcaatcg atgcagcaga acgtgcacaa ccagaatggg
    11941 aagcgttgcc tgctattgaa cgcgccagtt ggttgcgcaa aatctccgcc gggatccgcg
    12001 aacgcgccag tgaaatcagt acgctgattg ttgaagaagg gggtaagatc cagcagctgg
    12061 ctgaagtcga agtggctttt actgcggact atatcgatta catggcggag tgggcacggc
    12121 gttatgaagg cgagattatt caaagcgatc gtccaggaga aaatattctt ctgtttaaac
    12181 gtgcgcttgg cgtgactacc ggcattctgc cgtggaactt cccgttcttc ctcattgccc
    12241 gcaaaatggc tcccgctctt ttgaccggta ataccatcgt cattaaaccc agtgaattta
    12301 cgccaaacaa tgcgattgca ttcgccaaaa ttgtcgatga aataggcctt ccgcgcggcg
    12361 tgtttaacct tgttctgggg cgtggtgaaa ccgttgggca agaactggcg ggtaacccaa
    12421 aggtcgcaat ggtcagtatg acaggcagcg tctctgcagg tgaaaagatt atggcgactg
    12481 cggcgaaaaa catcaccaaa gtgtgcctgg aattgggggg taaagcaccg gctatcgtaa
    12541 tggacgatgc cgatcttgaa ctggcagtca aagccatcgt tgattcacgc gtcattaata
    12601 gtgggcaagt gtgtaactgt gcagaacgtg tttatgtaca gaaaggcatt tatgatcagt
    12661 ttgtcaatcg gctgggtgaa gcgatgcagg cggttcaatt tggtaacccc gctgaacgca
    12721 acgacattgc gatggggccg ttgattaacg ccgcggcgct ggaaagggtc gagcaaaaag
    12781 tggcgcgcgc agtagaagaa ggggcgagag tggcgttggg tggcaaagcg gtagagggga
    12841 aaggatatta ttatccgccg acattgctgc tggatgttcg ccaggaaatg tcgattatgc
    12901 atgaggaaac ctttggcccg gttctgccgg tggtcgcatt tgacacgctg gaagaggcta
    12961 tctcaatggc gaatgatagt gattacggcc tgacctcatc aatctatacc caaaatctga
    13021 acgtcgcgat gaaagccatt aaagggctga agtttggtga aacttacatc aaccgtgaaa
    13081 acttcgaagc tatgcaagga ttccacgccg gatggcgtaa atctggtatt ggcggcgcag
    13141 atggcaaaca tggcttgcat gaatatctgc agacccaggt ggtttattta cagtcttaat
    13201 gagtggaaga ggcggaggtt tttcctccgc ctgtgtgcgt cagagtttag cgaatttttc
    13261 gagggtgcga atgagttgtg tgacgaagcc atattcgtta tcgtaccagg cgaccgtttt
    13321 caccagttgt aaatcgccca cggcggtaat ttccgtttgc gtggcatcaa acaccgaccc
    13381 gaaatggctg ccaatgatat cggaagagac tatttcttca tcggtataac caaatgactc
    13441 gttattggtg gttgcttgtt taagtgcgtt attcacctct tcggcagtca ctttttttgc
    13501 gagaatggac accagctcag tgaccgaacc tgttttcacc ggcacgcgtt gcgcatgacc
    13561 tttcagtttg ccgctcagtt ccgggatcac cagaccaatg gcttttgccg cacctgtagt
    13621 gtggggaatg atattttctg ccgctgcgcg tgaagctcgt aaatccttcc cacgcggacc
    13681 atccaccagt gactgggtgc cggtataggc atgaatggtc gtcatcgtgc cgacttctat
    13741 cccgaaactg tcatgcaagg ctttggccat cggcgcaagg cagttagtgg tgcatgacgc
    13801 cacggaaaca atggtgtcgt tgccatccag agtgtcgtca ttgacgttat aaacgatagt
    13861 tttcatttca ccggcagggg cggaaatcaa caccttcttc gcaccagcat caagatgcgc
    13921 ctgcgatttc tcggcggagg tataaaagcc agtacattcg acaataattt ctgccccttt
    13981 cgctttccac ggaatatttt tagcctcttt ttcggcgtaa accgcgatgc tttttccatc
    14041 aacgataagt gaatcttccg taaaatcaac gctccagggg aacggtccgt agtttgaatc
    14101 atgtttcagc aggtaggcga gaatttttgg ggaagtgaga tcattaatag cgacaacgtc
    14161 tatgttgctt ttgacttcaa gtaatcgacg caacaccagt cgaccgatac gaccaaaacc
    14221 attaatacca actttactca tggtcttctc ctgtcaggaa cgttcggatg aaaattgatc
    14281 ctttccaggc ttagaccagg atggcgggat gggcaatcta cattcttgca gtgaaacgta
    14341 acgtactgaa aacgggtgaa caatatttaa tgaaatttta agaaaagccc gttatgttaa
    14401 cggcaaatta tgttaaagca ggaaatgtta tgggaaataa atactcaagg ttacaaatca
    14461 gcattcactg gctggtcttt ttgctggtta tcgtagcgta ttgcgcaatg gagtttcgtg
    14521 gtttcttccc acgtagcgat cggcccctca tcaacatgat tcatgtttcc tgtggcatct
    14581 caattctcgt actgatggtg gttcgtcttt tgttaagact gaaataccca accccgccga
    14641 ttatacctaa gccaaaaccg atgatgacgg gactggcgca tttgggccat ttggtgattt
    14701 atctcctgtt tattgcgctg ccagtgattg gtttggtgat gatgtataac cggggcaacc
    14761 cgtggtttgc gtttggtttg acgatgcctt acgcttcaga ggccaatttc gaacgggtag
    14821 atagcttaaa gtcgtggcat gaaatgctgg cgaatctggg atattttgtc atcgggttgc
    14881 acgctgcggc tgcactggca caccactatt tctggaagga caacacactt ctacgcatga
    14941 tgccgcgtaa acgttcctga aggatattta aagaaaacgc ctgtactaaa accgacccgt
    15001 ggtacaggcg aagaatacgg gtctacatcg gaagcgccta ttatatttat ttgtatgaca
    15061 aataaaacca taatccttgc ccatacgtcc atctggctta tttttaatca attcacccga
    15121 tctttgatct catcaacggt atcaaaataa accagcgtat aacgttaaaa tcgaacataa
    15181 gattcgataa accatggagg ttatatgaaa aaactggcac ttatcttggt tataggaacg
    15241 cttgtttcct tttatgccga tgccgggcgc aaaccctgtt ctggttcgaa aggggggatc
    15301 tcacactgta cggcaggcgg caaatttgtt tgtaatgatg gttctattag tgcatcgaaa
    15361 aaaacatgca ttaactgaag tgtaaaaggg gtgccatgag aaaatgaatc tgctagtcag
    15421 atgcgcgggg aaaatccccg cgcttgccct tacctggacg tgcaggccat gagcgcagca
    15481 acctccttat caccgtcccg gaaccgcagt tcgtagagcg tttgtcgggt caggagtgtg
    15541 aatgtcagaa tcgtaatgca gataatgagc agacacacca ccagagggtt gcgcttcata
    15601 gcctttctcc ttgccggatg gcgggtaaga ggctaagatc tgaattgctg tgttcattcg
    15661 ttggcctcgg ttgatagaaa tatcggtcgg ggccttcgtc tttctgattc ccggttagcc
    15721 tgaaaacaga aagtctcagg cacccgtgag catcctatgt ggtttcctta gggacgaaaa
    15781 taatcacctg acgaaattgc gtgctgtttt ccagaatttt tcgtcattcg ggttagccag
    15841 tttaatcatt cgttactctc ttcattccaa tggcattatt cttctctgcc ataattgttg
    15901 taaaaatgtg acgcaaagag gtttttggtc ataagtaatt accgtcaagt gccgatgact
    15961 ttctatcagg agtaaacctg gacgagagac aacggtaatg aatacaactc cctcacagcg
    16021 attaggtttt ttgcatcaca tcaggctggt tccgttattt gcctgcattc ttggcggtat
    16081 cttagttcta ttcgcattaa gttctgctct ggctggctat ttcctctggc aggccgatcg
    16141 cgatcagcgt gatgttactg cggagattga gattcggacc gggttagcga acagttcaga
    16201 ttttttgcgt tcagcccgga tcaatatgat tcaggccggg gcagcgagtc gtattgcgga
    16261 aatggaagca atgaagcgaa atattgcgca agccgaatcg gagattaaac agtcgcagca
    16321 aggttatcgt gcttatcaga atcggtcggt gaaaacacct gctgatgaag ccctcgacac
    16381 tgaattaaat caacgcttcc aggcttatat cacgggtatg caacctatgt tgaaatatgc
    16441 caaaaatggc atgtttgaag cgattatcaa tcatgaaagt gagcagatcc gaccgctgga
    16501 taatgcttat accgatattt tgagcaaagc cgttaagata cgtagcacca gagccaacca
    16561 actggcggaa ctggcccatc agcgcacccg cctgggtggg atgttcatga ttggcgcgtt
    16621 tgtgcttgcc ctggtcatga cgctgataac atttatggtg ctacgtcgga tcgtcattcg
    16681 tccactgcaa catgccgcac aacggattga aaaaatcgcc agtggcgatc tgacgatgaa
    16741 tgatgaaccg gcgggtcgta atgaaatcgg tcgcttaagt cgtcatttac agcaaatgca
    16801 gcattcactg gggatgacag tagggactgt tcgacagggt gcggaagaga tttatcgtgg
    16861 caccagcgaa atttccgctg gcaatgcgga cctttcatct cgcaccgaag aacaagcggc
    16921 ggcaatcgaa caaactgccg ccagcatgga gcaactcact gcgacggtga aacagaatgc
    16981 ggataacgcg catcatgcca gcaaactggc gcaagaggct tctattaaag ccagcgatgg
    17041 cgggcagacg gtttccggtg tagtaaaaac gatgggcgct atctccacga gttcgaagaa
    17101 aatttctgag atcaccgccg tcatcaacag tattgctttc cagacgaata ttctggcact
    17161 gaatgctgcc gttgaagccg cgcgagcggg tgagcaaggg cgtggatttg ccgttgtcgc
    17221 cagcgaagta cggacactcg caagccgcag cgctcaggcg gcgaaagaga ttgaaggctt
    17281 gatcagtgaa tcagtcaggt taattgacct gggatcggat gaggtggcca cggccgggaa
    17341 aaccatgagc actattgttg atgccgtcgc gagtgtcaca catatcatgc aggaaattgc
    17401 cgccgcctcg gatgagcaaa gtaggggtat aacgcaggtt agccaggcga tatctgaaat
    17461 ggataaggtg acgcaacaga atgcttctct ggtagaagag gcctcagcag cggcagtgtc
    17521 ccttgaagaa caggcggcac gattaactga ggcggtggac gtattccgtc tgaacaaaca
    17581 ttctgtgtcg gcagaacctc gcggagctgg tgaaccagtt agtttcgcta cagtgtgaaa
    17641 atgttaaagg agggatcgac agatcccttc acctttcaga acggcattga ttttcgaata
    17701 gcgttaatca tcaactggca accagaagag aacgtcgcat ctacgcgggt cagtattcca
    17761 atcggttcgc ctgcaccatg acccggaaca ggcagggcca ccagcgtggc atgacgcagg
    17821 tcgtctttta cagcgccaga agggacaaac cacacgtaat cgtattcaac cgtaagttga
    17881 cgagatagcg aagcagacag cgtttcgata caacccgaag gaattttaca tccctggctc
    17941 tgcactaatg catctgaatg ctggcgtggc gcagtgcctt ctggtgatac aacgaccggc
    18001 cattccagca cccggcttag cgttacgttc tcctgaagta gcggatgatt agggcggaca
    18061 accagcttca gcgattcaag aaacagcagt tcgtaattaa gtccggtcat cagttcagga
    18121 tctgacatcc gaccaatgcc gatatcgatt tccccggttt ttaaacccgc aagaatcata
    18181 gggttactca ttgtcgcaac ttgcaaggtc gtctcttttt gttgctgatg aaactgacct
    18241 ataaccgaag gtaatatccc cagtgccgca gtcggtagtg caccaaccct gacgacatca
    18301 ttattaagac cttctttacg atgaagcgac tgtccagcag tgttgatggc gtcaagcact
    18361 ctgactgcat gcgttaaaaa ttgttcgccg ggtaaggtaa gttgcgcccc ctgacgacca
    18421 cgctcaaaca agcgagcgcc cgtcagctgc tccagttcat tcaatgtctt agagagcgca
    18481 ggttgactca aattaagggt ttcagccgcg cgccccaaag ttccttgttg tgcgacagct
    18541 acgaatgtat gaaggtggcg caaacggatg cgctgactaa acagactatt tttttccata
    18601 agcgatgtta aaaacgaagc ggtgtcgctg acaagtgaag ttgtttgatt atgataactt
    18661 gattgcaaaa tattattaac aattaaagca attatgttac agcgaaatgg ataatattga
    18721 tgttttcgcg gcgagatcac agtttgcaaa ttcttcccgc cagagtgaat gcggttacct
    18781 acactccaga ttactgacca ctggaggcag acattatggc gaacagcatc acggcggatg
    18841 agattcggga acagttttcg caggcaatgt cagccatgta ccagcaagaa gttccgcagt
    18901 acggtacgct gctggaactg gtagctgatg tgaatctggc tgtgctggaa aacaatcctc
    18961 aactgcacga aaaaatggta aatgcagacg agctggcgcg actgaatgtt gaacgtcatg
    19021 gggcgattcg tgttgggact gcacaagagc ttgctactct tcggcggatg tttgccatta
    19081 tggggatgta cccggtgagc tattacgatc tctcgcaggc gggggtgccg gtacattcca
    19141 cagcatttcg gcccattgat gatgcttctc tggcgcgtaa tcccttccgc gtttttactt
    19201 cattactccg ccttgagctt atcgagaacg aaattttgcg ccagaaagcg gcggagattc
    19261 tgcgtcagcg cgatatcttc accccacgtt gtcgacaact gttagaggaa tatgagcagc
    19321 ggggcggttt taacgaaaca caggcacagg agtttgtgca ggaagccctg gaaacgtttc
    19381 gttggcacca gtcagcaacg gtagatgaag aaacctatcg cgccttgcac aacgaacatc
    19441 ggttgattgc tgatgtggtc tgttttcctg gatgccatat caaccacctg acgccacgca
    19501 cgctggatat tgaccgggtg cagtcgatga tgcctgaatg cggaattgaa cccaaaattc
    19561 tgatcgaagg gccgccgcgc cgcgaggtac cgattttact acgccagact agctttaaag
    19621 cactggaaga gacggtgttg tttgcagggc agaaacaggg cacgcatacc gcgcgctttg
    19681 gtgaaataga acagcgtggc gtggcattaa cgccgaaagg acgacaactg tatgatgatc
    19741 tactgcggaa cgctggaacc gggcaggata atctcactca ccaaatgcat ttacaggaaa
    19801 ccttccgcac ttttcctgac agtgagtttt taatgcgtca gcaagggttg gcatggttcc
    19861 ggtaccgtct gacgccttca ggtgagacgc atcgtcaggc gattcatccc ggagacgatc
    19921 cacagccctt aattgaacgt ggttgggtag tggcgcaacc cattacctat gaagatttct
    19981 tgcctgttag cgcggcgggg atcttccagt caaatctggg taatgaaacg caggcacgca
    20041 gtcacggtaa tgccagtcgc gaagcatttg agcaggcgtt gggctgcccg gttttggatg
    20101 agttccagct ttatcaggaa gcggaagaac gcagtaaacg tcgctgtggt ttgctttaaa
    20161 ttctgaccat ccgcctttgc aaaaatttgc ctgattttac aaacgaatca ggctcatccc
    20221 atcgacataa aaaaattgcc gatttatgca tattctctca gttcaacaat tggattatta
    20281 ataaatattg tctagagtga gcggtcataa ataagcactt tcttgccgct gaaaacgatc
    20341 agcgcgggac cattcacaac accagaagga ctcactttca ggtatggatc gtagacgatt
    20401 tattaaaggt tcaatggcta tggccgccgt gtgcggtacc agcggcattg cttctctttt
    20461 ttctcaggcg gcattcgcgg cagattctga tattgccgac gggcaaaccc aacgttttga
    20521 cttctccatt ctacagtcaa tggcgcacga cttagcgcaa acagcgtggc gtggtgcgcc
    20581 gcgtccgtta cctgacactc tggcgacaat gacgccgcag gcttataaca gtattcaata
    20641 cgacgccgaa aaatcgctct ggcataacgt tgagaaccgt caactggacg ctcagttctt
    20701 ccatatggga atgggattcc gtcgccgcgt tcgtatgttt tctgtagatc cagcaacaca
    20761 tctggcgcgt gaaattcact ttcgcccgga gttgttcaaa tacaacgatg cgggtgttga
    20821 taccaaacaa ttagaagggc aaagtgatct cggttttgcc ggttttcgcg tgtttaaagc
    20881 ccccgaactg gcgcgccgtg atgtcgtatc attcctcggc gcgagttatt tccgcgcggt
    20941 tgatgataca tatcaatacg gtttgtcggc ccgcggcctg gcgatcgaca cttacaccga
    21001 cagtaaagaa gagttccccg actttactga cttctggttt gatacggtaa aaccgggggc
    21061 aaccaccttt accgtttatg cgttgctcga tagcgccagc attactggtg cctataagtt
    21121 cactatccat tgtgagaaaa gtcaggtgat tatggatgtg gaaaatcacc tgtatgcgcg
    21181 caaagacatt aaacagctgg gcattgcgcc gatgaccagc atgttcagct gcggtactaa
    21241 tgaacgtcgg atgtgcgaca ccattcatcc gcaaatccat gactctgatc gtttgtccat
    21301 gtggcggggc aatggcgagt ggatttgtcg tccgctgaat aatccgcaaa aattgcagtt
    21361 caatgcttac accgacaaca acccgaaagg gtttggttta ttgcaactgg atcgtgattt
    21421 ctcccattat caggacatta tgggctggta taacaaacgc ccaagtctgt gggtggagcc
    21481 gcgtaacaag tggggtaagg gcaccatcgg cctgatggaa atcccaacaa cgggcgaaac
    21541 gctggataac attgtctgct tctggcagcc agaaaaagcg gtaaaagcgg gtgatgagtt
    21601 tgcattccag tatcgtctgt actggagtgc gcaaccgcct gttcattgcc cattagcgcg
    21661 cgttatggcg acgcgtaccg gcatgggcgg tttcccggaa ggttgggcgc caggtgaaca
    21721 ctatcccgaa aaatgggcgc gacgttttgc cgtcgatttc gttggtggtg atctgaaagc
    21781 tgccgcgcca aaaggcattg agccggtgat tacgctttcc agtggggaag cgaaacaaat
    21841 cgaaattctc tatattgaac ccattgatgg ttatcgtatt cagtttgact ggtatccgac
    21901 ttcggactcc actgatccgg tcgatatgcg gatgtatctg cgttgtcagg gcgacgctat
    21961 cagtgaaaca tggctgtatc agtatttccc gccagcgccg gataaacgtc agtatgttga
    22021 tgaccgcgtg atgagttaat cgttttttct tcggcatctt cttcgggagg tgccgtctgg
    22081 ttaaacacga tcccgctcgc atttttccct aagttaaatg agtaatctga tggtgtgtat
    22141 ttcagataca ccttgtcagc cactaacagg gagtgcgtat gtttccagaa taccgagatt
    22201 taatatcccg tctgaaaaac gaaaatcctc gctttatgtc cttgttcgat aaacacaata
    22261 aacttgatca tgaaattgcc agaaaggaag gttccgacgg tcgagggtac aatgcggaag
    22321 tggtccgcat gaaaaaacaa aagctacagt taaaagatga gatgctcaaa atcctgcagc
    22381 aggtgagcgt caaagaggtg taaactttcc taagccgcca gccaggcggc ttttttaaca
    22441 actgcatgga ttgactggag ataagatgac tgaaacgata aaagtaagtg aatcacttga
    22501 attacatgct gttgcagaaa gtcatgtcac acctctttat cagttaatct gtaaaaatag
    22561 aacctggtta cagcagtcgc taaactggcc gcagtttgtt caaagtgaag aggacacgcg
    22621 aaaaacggtg cagggtaatg tgatgttgca tcaacgcggc tatgccaaaa tgttcatgat
    22681 tttcaaagaa gatgaactta tcggcgttat ctcgtttaat cgtattgaac cactgaataa
    22741 aaccgctgaa atcggctact ggctggacga atctcatcag gggcagggga tcatttctca
    22801 ggcgctgcag gcattgattc atcattacgc tcagtctggt gaacttagac gcttcgtgat
    22861 caaatgtcgg gtggacaatg cgcaaagcaa ccaggtcgct ttgcgcaatg gttttatcct
    22921 tgaaggttgt ctgaaacagg ctgagttcct gaatgatgcc tatgatgatg tgaacttata
    22981 cgcgcgtatt atcgattcac aataaccctg ccagcggcgt tcgcgtaatg cgctcctcgc
    23041 cgttaatgac ttttggacca cggacatgaa ccgtgtcgcc atcaaaggct tcaacgacgg
    23101 cgtggtcggt taactccaca tgtttttcaa ttatcaccgc acctgtaata cggctctcac
    23161 cttgtattac aacgtgctcg tcgagcagaa tcggtccgcc acgtaccacg gcatttccgc
    23221 caatcaagac atgatgtttt aacacacaat taccttccac aatggcatat tccgccacct
    23281 gcgaactgta atgaatcgtc ggaatggcat cttcttctat gccagctttc acctgcgcgt
    23341 gaccgtagac tttagcacaa tcgcatagcc agacgttgtt ctcttcatta ccttcaacgc
    23401 tggcaaaatc aaaaacttcg gcgcgatgtt caataaaagc ataccggacg acggcatcgc
    23461 cataaatttg tgcctggtga acaatacgcg aggcgctaac ccttgcgcga tcataaattt
    23521 gtagcaggag ttgatggtcg ggcgttaagc cttgtgcggc gacgatcata gaatgttgat
    23581 caattaaggc gtgaccaaat attctacatt gcccgcaaac taatgagtca cgaatggtga
    23641 cactgtcact tatatatgca ccctgactga tttcactatt atcgatccag acgttatccg
    23701 ttgcgtaaac ctctccccac agtacgctgg tgccggtgat gcgcgtatta ccggaaatca
    23761 ccgctccgcc gaacgcaata gcattctggt cgtaaatcca gcaatttcct tcctgtgcta
    23821 acaccgtttc gcaatcgatc cagccgccag cggttcctgc tatcacatcg ttaaaatcgc
    23881 tcatggcgat aatctgccgt aataacacac tttttttagt gccatcatcc tgataactaa
    23941 aggctcgctg ttcttcactt agacgatatt tgcgcatagc gttttcccac aggtgactta
    24001 ctataaccgt agcaaattct gcggctctgg ctatgctcga gaaaattcca taaaatgcat
    24061 ttcaaatata ctttataaat taaacaaaat gagtaagaag atgcagagcg ataaagtgct
    24121 caatttgccg gcaggctact ttggtattgt gttggggacg atagggatgg gatttgcctg
    24181 gcgctatgcc agccaggttt ggcaggtcag ccactggtta ggggatgggc tggtgattct
    24241 ggcgatgatc atctggggat tattaactag cgcatttatt acccgactca tacgctttcc
    24301 gcatagcgtg ctggcggaag tgcgccatcc agtgctgagc agttttgtga gtttgtttcc
    24361 ggcaacgacg atgctggtgg cgattggttt tgttccgtgg tttcgcccac tggcggtgtg
    24421 cctgttcagt tttggcgtcg tggttcagtt ggcttatgcc gcctggcaaa ctgcgggatt
    24481 atggcgcgga tctcaccctg aagaagctac cacgcctgga ctgtatctgc cgacagttgc
    24541 caacaacttt atcagcgcaa tggcctgtgg tgcgttgggc tacaccgacg ccggtctggt
    24601 gtttttaggc gcaggcgttt tctcatggct aagccttgaa cctgtgatct tgcagcgtct
    24661 gcgcagttcg ggagaattac ccacggcgct gcggacatca ctcggcattc agctcgctcc
    24721 tgcgctggtg gcctgtagtg cctggctgag cgtcaacggc ggcgagggtg acacgctggc
    24781 gaaaatgctt ttcggttatg gactgctgca actgctgttt atgctacgtc tgatgccatg
    24841 gtatctctcc cagccattta atgcttcatt ctggagtttc tcgttcggcg tatctgcact
    24901 ggcaaccacc ggtttgcatc tggggagtgg cagcgataat ggatttttcc atacgctggc
    24961 ggtgccgctg tttatcttta ccaattttat tattgcaata ctgctcatcc gtacttttgc
    25021 gcttctgatg cagggaaaat tgttaatcag aaccgagcgc gccgttttaa tgaaagcaga
    25081 ggacaaagaa tgatcattcg tgacgaaaac tattttactg ataaatatga attaactcgc
    25141 acacactctg aagtgctgga agcggtgaaa gtggttaaac cgggtaaaac gctggatctg
    25201 ggctgtggca atggtcgtaa cagtctttac ctggcagcca atggttatga tgttgacgca
    25261 tgggataaaa atgccatgag tatcgccaac gtcgagcgga ttaaatccat tgaaaatctg
    25321 gagaatttac acacccgggt cgttgatctg aataacctca catttgatgg acagtatgat
    25381 tttattcttt cgactgtggt gctgatgttc cttgaggcta aaaccatccc agggctgatt
    25441 gccaatatgc aacgttgcac taaacctggc ggttacaacc tgattgtggc ggcgatggat
    25501 accgctgatt atccatgtac cgtcggcttc ccgtttgcct tcaaagaggg ggaattacgt
    25561 cgatattacg aaggctggga gatggtgaaa tacaatgaag acgtcggcga gctgcaccgc
    25621 accgacgcca acggtaatcg tattaaactg cgtttcgcca cgatgctggc acgtaaaaaa
    25681 tgacccgcta ggaacaaggc gcgtgaaaat tccccacgct gagatggttt actgttcttc
    25741 ttttcggtaa gcatattttt tatcgaatgg atgtgaaatt aatcacagta gtcgaaattt
    25801 tttagcagct taacttactg aaatttaact actgatgatt gacttagccc ctttttcggc
    25861 agcccctttt tcggcattga ctatgtcttt ttcggcattg actatgtcgt ctgaaaag
//
LOCUS       DAZGAP010000066        25905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGAP010000066 DAZGAP010000000
VERSION     DAZGAP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.066"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1634
                     /gene="yqiK"
                     /locus_tag="ACHLAM_002251"
     CDS             <1..1634
                     /gene="yqiK"
                     /locus_tag="ACHLAM_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7853770.1"
                     /translation="WMFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVM
                     SGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGI
                     ATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAE
                     DLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVE
                     VAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEAERRREAEQTR
                     ILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQ
                     SQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKA
                     EKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALP
                     AVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQALSAALSYRTQA
                     PLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            1874..1933
                     /locus_tag="ACHLAM_002252"
     CDS             1874..1933
                     /locus_tag="ACHLAM_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7853771.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(2053..3486)
                     /gene="hldE"
                     /locus_tag="ACHLAM_002253"
     CDS             complement(2053..3486)
                     /gene="hldE"
                     /locus_tag="ACHLAM_002253"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO7853772.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNQ
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3534..6374)
                     /gene="glnE"
                     /locus_tag="ACHLAM_002254"
     CDS             complement(3534..6374)
                     /gene="glnE"
                     /locus_tag="ACHLAM_002254"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO7853773.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(6397..7698)
                     /gene="ygiF"
                     /locus_tag="ACHLAM_002255"
     CDS             complement(6397..7698)
                     /gene="ygiF"
                     /locus_tag="ACHLAM_002255"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO7853774.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7940..8560
                     /gene="ygiM"
                     /locus_tag="ACHLAM_002256"
     CDS             7940..8560
                     /gene="ygiM"
                     /locus_tag="ACHLAM_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO7853775.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8624..9862
                     /gene="cca"
                     /locus_tag="ACHLAM_002257"
     CDS             8624..9862
                     /gene="cca"
                     /locus_tag="ACHLAM_002257"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO7853776.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(10050..10871)
                     /gene="bacA"
                     /locus_tag="ACHLAM_002258"
     CDS             complement(10050..10871)
                     /gene="bacA"
                     /locus_tag="ACHLAM_002258"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7853777.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10962..11330)
                     /gene="folB"
                     /locus_tag="ACHLAM_002259"
     CDS             complement(10962..11330)
                     /gene="folB"
                     /locus_tag="ACHLAM_002259"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO7853778.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11435..12052
                     /gene="plsY"
                     /locus_tag="ACHLAM_002260"
     CDS             11435..12052
                     /gene="plsY"
                     /locus_tag="ACHLAM_002260"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO7853779.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12065..12997)
                     /gene="ttdR"
                     /locus_tag="ACHLAM_002261"
                     /gene_synonym="dan"
     CDS             complement(12065..12997)
                     /gene="ttdR"
                     /locus_tag="ACHLAM_002261"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO7853780.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13204..14115
                     /gene="ttdA"
                     /locus_tag="ACHLAM_002262"
     CDS             13204..14115
                     /gene="ttdA"
                     /locus_tag="ACHLAM_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO7853781.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14112..14717
                     /gene="ttdB"
                     /locus_tag="ACHLAM_002263"
     CDS             14112..14717
                     /gene="ttdB"
                     /locus_tag="ACHLAM_002263"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO7853782.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14766..16229
                     /gene="ttdT"
                     /locus_tag="ACHLAM_002264"
     CDS             14766..16229
                     /gene="ttdT"
                     /locus_tag="ACHLAM_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO7853783.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16272..17285)
                     /gene="tsaD"
                     /locus_tag="ACHLAM_002265"
     CDS             complement(16272..17285)
                     /gene="tsaD"
                     /locus_tag="ACHLAM_002265"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO7853784.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17523..17738
                     /gene="rpsU"
                     /locus_tag="ACHLAM_002266"
     CDS             17523..17738
                     /gene="rpsU"
                     /locus_tag="ACHLAM_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO7853785.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17849..19594
                     /gene="dnaG"
                     /locus_tag="ACHLAM_002267"
     CDS             17849..19594
                     /gene="dnaG"
                     /locus_tag="ACHLAM_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO7853786.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDNQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19789..21630
                     /gene="rpoD"
                     /locus_tag="ACHLAM_002268"
     CDS             19789..21630
                     /gene="rpoD"
                     /locus_tag="ACHLAM_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO7853787.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(21708..22214)
                     /gene="mug"
                     /locus_tag="ACHLAM_002269"
     CDS             complement(21708..22214)
                     /gene="mug"
                     /locus_tag="ACHLAM_002269"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO7853788.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22339..22414
                     /locus_tag="ACHLAM_002270"
     tRNA            22339..22414
                     /locus_tag="ACHLAM_002270"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22372..22374,aa:Ile,seq:cat)
     gene            complement(22468..23232)
                     /gene="nfeF"
                     /locus_tag="ACHLAM_002271"
                     /gene_synonym="yqjH"
     CDS             complement(22468..23232)
                     /gene="nfeF"
                     /locus_tag="ACHLAM_002271"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO7853789.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISVGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            23521..24144
                     /gene="nfeR"
                     /locus_tag="ACHLAM_002272"
                     /gene_synonym="yqjI"
     CDS             23521..24144
                     /gene="nfeR"
                     /locus_tag="ACHLAM_002272"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO7853790.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24248..25768)
                     /gene="aer"
                     /locus_tag="ACHLAM_002273"
     CDS             complement(24248..25768)
                     /gene="aer"
                     /locus_tag="ACHLAM_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO7853791.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
BASE COUNT         6218 a         7131 c         6625 g         5931 t
ORIGIN      
        1 cctggatgtt taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg
       61 ccaggctcta tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc
      121 aaaaagtggt aatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca
      181 tcaatatgaa tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga
      241 aagatcgtat gcgcgtcgat gtcgttgtcg ctttctttgt gcgggtaaaa ccgtcagtgg
      301 aaggtattgc caccgccgcc cagacgcttg ggcaacgcac cctgtcgcct gaagacttac
      361 gtatgttggt tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca
      421 tgcatgagtt acaggatacc cgcgagaact tcgtgcaggg cgtacaaaat accgtagctg
      481 aagatctgtc gaaaaacggc ctggaactgg aaagcgtttc acttaccaac tttaaccaga
      541 cgtcgaaaga acatttcaat cctaacaacg cctttgacgc cgaaggttta actaagctga
      601 cgcaggagac ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg
      661 cggtgcgtga gaaaaaccgt gatgcgcttt cgcggaagct ggagattgaa cagcaagaag
      721 cgtttatgac gcttgagcag gagcagcagg ttaaaacccg taccgctgag cagaatgcga
      781 aaattgcggc ttttgaggcc gagcgtcgtc gggaagcaga gcagacgcga attctggctg
      841 aacgacagat tcaggaaacg gaaatcgacc gcgaacaggc cgtccgttcc agaaaagttg
      901 aagctgaacg tgaagttcgc attaaagaga tcgaacagca gcaggtcacc gaaatcgcca
      961 accagacgaa atcgatcgcg attgccgcca aatcggaaca gcagtcacaa gcggaagcgc
     1021 gtgccaacct tgcacttgca gaagcggtaa gcgcccagca aaacgtagaa accactcgcc
     1081 agactgccga agccgatcgt gctaaacaag tggcgttgat cgccgcagcg caggatgcag
     1141 aaaccaaagc ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagaaatgc
     1201 aggcggcggc tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag
     1261 aagcacaacg tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta
     1321 aattcaaact ggcattattg caggcgctgc ctgcggtaat tgagaaatcc gtggaaccaa
     1381 tgaagtctat cgacggcatc aaaattattc aggtcgatgg gctgaatcgt ggtggcgcta
     1441 cgggtgatgc aaacacgggt aatgtggggg gcggaaacct ggcagaacaa gcgttatcag
     1501 ccgcactctc ttaccgtaca caggcaccgc tgattgactc cttgctcaat gaaattggcg
     1561 tttcaggcgg ctcactggcg gcattgactt catccttaag ctcaacaact cccgtcgaag
     1621 aaaaagcaga gtaaaccctc ttctatgaat aatccctggc ccaaaaagcc ggggattttt
     1681 tatatctgcg ttccgctaaa aggtgcaaat gatcaagctg ttgcagcatt ttgcgtgact
     1741 gctcggggaa ggcaaaactg cctctgggaa agcgttgcgc ggggtccggc gctcatcaac
     1801 aatcggggag tcagcaaggg gctgaaacgg gaaagcccct cccgaggaag gggccttgta
     1861 taaggaaagg gttatgatga agctcgtcat catactgatt gtgttgttac tcgtaagttt
     1921 cgcagcttat taacagccaa tcagaggggg gagaaatcct ccctcaccct tgttccttta
     1981 ctttaggttg aaaaaaaatc agcgtcaata ggcccgcctg ctacaaagcg agatctgtga
     2041 accgctttcc ggttagcctt ttttatcctg ttggatcttc ttgatgatgt tggtcgtcga
     2101 gcaaccgtct tcaaagttga gcaccagcac ttcaccaccg ttggcccaga cttctttact
     2161 cccggcaatc tcttctggtt tatagtcgcc gcctttcacc agtagatctg gcaagatacc
     2221 ggcgatcaag cgctgcggcg tgtcctcttc aaacgacacc acccagtcga ccgcttccag
     2281 tgcgcccagc acaatcatac gctgttcgag ctggttaacg gggcgggaat cccctttcag
     2341 ccgtttggtg gaggcatcgc tgttcacagc gacaatcaaa cggtcaccca gtttacgggc
     2401 atttgccaga taagagacgt gcccagcgtg caggatgtca aagacaccgt tggtcatcac
     2461 cactttttca ccgcgtttac gcgctgccgc tacagcaagc ttcagttcct cttcggtcat
     2521 cacaccaaag ccggtatctg cacgtccacg tacagcgttt tccagctcga tcggcgaaac
     2581 cgtggatgtc cccagtttgc cgaccaccac accagccgcc gcattggcaa agaagcaggc
     2641 ttcttccagt gaattacccg ctgccagcgt tgccgccagg acgccaatca ccgtgtcgcc
     2701 cgcaccggta acgtcataca cttcctgcgc ctgggttggc atatgcagtg gcgctttacc
     2761 cggttgcagc agcgacatac cctgttcgga acgggtcact aacagagccg agagttcgta
     2821 atcggcaatc agtttcatgc cgcgctcaac aatctcttct tcggtcttac atttaccgac
     2881 aacagcttca aattccgaga gattcggcgt taacagcgta gcgccgcggt agcgctcaaa
     2941 atcggtacct tttggatcaa tcagcaccgg aacacccgct ttacgcgcca gttggatcat
     3001 ctgctgtacg cttgccagcg cacctttggc gtaatcagaa agcaccagcg cgccaatcga
     3061 actcagtgcc tgattaatcc gctcgtgcag cggctgcgga tcaacccctt cgaaaccttc
     3121 ttcgaaatcc agacggatca gttgttggtt gcgggaaagt acccgtaatt tggtgatggt
     3181 cggatgcgtc ggtacagaaa caaagtcgca tttgacgttg acgtcggcca gagatttact
     3241 gagcgcgcgc gccgcatcgt caatgcccgt caacccgacc aggcgtgcat tagcaccgag
     3301 agaagcgata ttcatcgcca cgttagccgc gccgcccgga cgttcttcga tggtattcac
     3361 tttaaccacg ggcaccggcg cttccggcga gatacggctg gtgggaccgt accagtaacg
     3421 atccagcatc acatcaccaa ccaccatcac tcctgcacgt tcaaactctg gcagcgttac
     3481 tttcattcct gtctcctgag agattcaaaa tttgcgcgcg ataataccat acttcattct
     3541 tccaccagcc acttctgcca gcttgcccgc accagttcac gctctgcggt gaagcaatcc
     3601 tccgacacat gacccggcaa ttcctgtaat gccagatgat gaagttcatc gcgcagcgtc
     3661 gtgtaagcac gggtcagcgc catcgcttcc tgctcttcca taatgtcgtt ttgcgccagt
     3721 agttccagaa tgcgcacgtt gtctgaccag cgcgttaact tcggtttttc gtgagcgtag
     3781 cgcaacacca gatattgagt aataaattcg atatcggtaa ttccgccttc atcggctttg
     3841 atatcaaagc gatcgcgatg tttattgccg agatgggcac gcattttctc gcgcatttcc
     3901 cgcacttctg tttgcagggt tttgccttcg cgcggcagcg tcataatctc ccggcgtact
     3961 gcgtcaaagt gcgcggtgag ctgcggatcg ccgtacacta cacgcgcacg caccagcgcc
     4021 tgatgctccc acgtccaggc ctcgtttttc tgataatcag caaatgcttc tgcggatgtc
     4081 accagcattc ccgccgcccc ggacggacgc agtcgtgcat ccacttcata caaaatgccg
     4141 gaagaggttc gcgtactgaa cagatgcata atgcgttgcg ccagacgcag ataaaactgt
     4201 cgcccgtcga tttcccgctc gccgtcagtc atcgcatcca ttgggcaatc atggaggaag
     4261 atcaggtcaa gatcggaact gtagcctaac tcccagccac ccagcttgcc gtagccgacc
     4321 accgcaaaac cacgcccttc gcgttcgttc aggtgatttg gcttaccgta gcgggcaacc
     4381 atttgaaccc acgcctgctg aacgacggca tcaatcatgg cttctgccag ccaggttaag
     4441 tgatcgctca ctttcattac cggtagcgta ccggcgatat ccgctgccgc aatgcgtaac
     4501 agctgcgcct gtttgaactg acgcagcgcc tcaagctgtt gttcttcgtc atcttccggc
     4561 acgcgcagca aatactggcg cagttcatca cggtaggcat ccgtcgccgt tggttggtag
     4621 agggtatttg gatcgagcaa ttcatccagc aataatggat aacgcgctag ctggctggca
     4681 atcatcggcg atgcagcaca cagagaaatc aaatgtttaa gcgccgcagg gaattcacta
     4741 agcagctcaa gataagtggt gcgagtaaca atccccacca gcaaggcggt aatgcgcgac
     4801 agcgtgacag cagcgtcttc acgcgcacag acatcactta gcagatgtgg catcagatgg
     4861 tcgagcacct gacgtcctcg cggcccgatg gtgcgcttat ccagctcttt gcggaaatca
     4921 gcaatcagca ttagcacctg tttgcgatca tcctcgctaa gatgcgccag cactggcgtg
     4981 gtgtcatctt cctgcaacgc atcctgccac agctcacgcc actgttccga cagcgactct
     5041 tcctgagttt cactttcgtc gtcgccaatc aattcattaa acacccggcg cacattggcc
     5101 atatgtgcgg tcagcacccc ggtcaattgc ggccagtcag caaagtccat cgcccacgcc
     5161 agccgcgcac ggttaagctc atcaaaagga agcgtctggg tttgttcgtc gttaatgctt
     5221 tgcagcaggt tttccagacg ccgcaggaac agatacgcca ctcgcaattg ttcagcatcg
     5281 ttttcagaaa gcagatgcag cgcggcaatg gcgctgagcg ttggcagtaa agagcgcgat
     5341 tgcagcgacg gttcgcgccc gccgcgaatg agctggaaca cctggacgat aaattcaatt
     5401 tcgcgaatgc cgcctgcgcc aagtttgatg ttgtcggtca aaccgcgtcg acgcacttca
     5461 cgggcaatca ttcctttcat gttgcgcagc gactgaatca cgctgaaatc gatgtaacga
     5521 cggaaaacaa acggacgcag catcgcacgc aactcgttag catagacgcc gtcgctatcc
     5581 cccataatcc gcgccttgac catcgcgtaa cgctcccagt cgcgcccctg ctcctggtaa
     5641 taatcttcca gcgccgcaaa actcagcacc agcgggccac tttcaccaaa cggacgcagc
     5701 cgcatatcca cgcgatagac gaaaccatcc tgcgttggtt gatccagcac tttaatcagc
     5761 cgctgcccca tgcgggtaaa aaactgcgcg ttatccagtt cccggcgtcc gccctgggta
     5821 caaccatgtt ccggccaggc aaaaatcaga tcgatatctg aggagaaatt cagttcccca
     5881 ccgcccagct tacccatgcc taaaatcagc agcggttgcg cttcgccctg cgcattgcac
     5941 ggcgttcccc attcgcggca gcaggcgtca tacagccagt cacgcgccgc aacaatcagc
     6001 gtctccgcca gatggctgag ctgctgcaat atgctctctt cagtaaccag tgccagcgtt
     6061 tgcgcccagg cgatgcgcac cataatgcgc cgccggaata gccgcagctc gcgcattaac
     6121 ccggcttcgt cactcacatt actgagcgcc tcctgcaacc atgctgcgta atgctgccat
     6181 tcgtcggcct gcggcggttg gctttccagt tccgtcagcc actctggatg cgcaattatg
     6241 ctgtcctgca gaaaatcact gaaagtaagt actgacttcg cctgtgcgct aagtgattcc
     6301 tcggctaaag gctctggcag ccgctcaaca acggtctgcc agtactgctg taacggtgaa
     6361 gagagcggct tcataagcga ttttatcctt gcctgattaa cgttttccgc tgtgcaacca
     6421 gaacggttcc tgattgtttg cctcattacg gaaatgttca atttcaatgc gttgcccggt
     6481 cgcaatagcg tgacgcagcc cctgccagtt ctccagccag gcttgcgcga cgacaggatc
     6541 gtagtaaccc gccagcaaca ggattgagtc aatatcacgc gtcaggcgtg gcaactggtc
     6601 acggtagcga tcgcccaacg gctggcagaa aacgcttttc agttcagcgg catggcggga
     6661 aagatggata tcggcaaagc gtttgaagga atcgctcatt ttactctgcg ctttggcatc
     6721 taaaaacggc tgccaggctt tgcttaccag ccattcggtc aatgccagct tcgccattgc
     6781 cgtttcggta gagtagaccg ccgtcacggc agaaaccgct gaagcgatgg tcgcttcgca
     6841 ttgagtcaat aaatcacgta agtgagtgct cgctttacgc ggcacaatgc caccgaacag
     6901 catcagcgta tgacggacca ggccaatggc tgccagcacc tgctctttcg ccgcatcatt
     6961 gccgcgtacc catagttctt catgatactg ccattgcgct aacgccagct cgagcgccgc
     7021 ttccagcccc tgttccacat cagcttttgc cgcaacatgt aaaatggtgg tcggtttgat
     7081 ttcacgcgcc ggattgccct gcgccagatg atagccacgc gccgctttgc tcagactgcc
     7141 ctggcgtaat ccggtttgcg ataccagttg gttcgccagt ttcagcaccg cgcgcgtgtc
     7201 gccgctaagc agttccagtt ccagctcgca gataggttca gcaaattcac ccgctttcac
     7261 ttccccctgg tcgagggcga tttcaatttg gctaccatca acctctacca gccacttttc
     7321 gcgataaaaa tcggtgctaa acagcggctg cacgcgggaa gcgagatcgg taggcaattc
     7381 gccgttcggc cagacttccg tcggtaactg cgccaggtcg agcgtcggtt cgctcaacgc
     7441 cacgttatat tccgggcgct ggtgtaagcc gcctgtcact cttcctgcaa ctttcatggt
     7501 catctcatag cgaccgtttt cgccacgaat acgcaagccc atgtcgtgcc cacgcagcca
     7561 gttatccggc gtttcgtagt aaatattcag caactgcacg gggtcatggt gctcgccgcc
     7621 cagcgtattg agatggtcac gcaacgcctc aacggcactg tgattaacaa taaactttaa
     7681 ttcgatttcc tgagccatag cctttactta tcggttatgt cacataagtg acgatgaacg
     7741 gcgaacttaa tgcgatcttt tttgtcagta gatagtattt tgcgccaaat tgccatgcaa
     7801 cgggcaattt gacgggcgta aaagtttgaa gcagtggcaa gacctgacac agaggattcc
     7861 gattgatgtc gaatgctttg cgtagcgaca ctgatgccac tactatcgtt ccactttcca
     7921 tgacaataac gacagcctga tgccaaaatt acgcctgatc ggattaactt tactcgcact
     7981 tagcgccact gccgtctcac acgctgaaga aacgcgctat gtttctgatg aactgaatac
     8041 ctgggtccgt agcggtccgg gagatcatta tcgcctcgtg ggcacggtta acgccggcga
     8101 ggaagtgacc ttattacaaa ctgacgccaa caccaattat gcccaggtga aagacagctc
     8161 tggccgtacc gcctggatcc cgttgaaaca acttagcaca gagccaagcc tgcgctcccg
     8221 tgtgccagat ctggaaaatc aggtcaaaac cctgaccgat aaactcacca atatcgataa
     8281 cacctggaac caacgcaccg cagaaatgca gcaaaaagtg gcgcagagcg acagcgtgat
     8341 caacgggtta aaagaagaaa atcagaaact gaaaaacgag ctgattgtcg cgcagaaaaa
     8401 ggtcgatgcc gccagcgtac agctggatga caaacagcgc accatcatca tgcagtggtt
     8461 tatgtatggc ggtggcgtgc tggggcttgg cttgctgctt ggtctggtac tgccgcacct
     8521 gatcccaagc cgcaaacgca aagatcgctg gatgaactaa atcgccttct tcgttgcact
     8581 gtcttattat taggaattgt gtatttgacg aagggatgat ggcgtgaaga tttatctggt
     8641 cggtggtgct gttcgggatg cattgttagg gctaccggtc aaagacagag attgggtggt
     8701 ggtcggcagt acgccacagg agatgctcga cgcgggctac cagcaggtag gccgcgattt
     8761 tcctgtgttt ctgcatccgc aaacgcatga agagtatgca ctggcacgta ccgaacggaa
     8821 atctggttcc ggttacaccg gttttacctg ctatgccgca ccggatgtca cgctggaaga
     8881 tgatcttaag cgtcgcgatc tgaccattaa tgcgctggcc caggacgata acggcgagat
     8941 tatcgacccg tacaacggtc tgggcgatct gcaaaatcgt ctgttgcgcc atgtttcccc
     9001 cgcttttggc gaagatccgt tacgcgtgtt gcgcgtagcg cgttttgctg cgcgttatgc
     9061 ccacctcagt ttccgtattg ccgatgaaac tctggcgctg atgcgcgaga tgacccatgc
     9121 gggtgaactg gaacacctga cgcctgaacg ggtatggaaa gagacggaaa acgccctcac
     9181 cacccgcaat ccccaggtgt tcttccaggt actgcgcgat tgcggcgcac tgcgcgtttt
     9241 attcccggaa attgacgccc tgtttggcgt tccggcccct gccaggtggc atccggaaat
     9301 cgacacgggt attcatacct taatgacgct ctcaatggcg gcgatgctga gtccgcaggt
     9361 cgatgtccgt ttcgcaacgt tatgccacga tctcggtaaa ggactgacgc caccagaact
     9421 ttggccgcgt catcatggtc atggcccggc gggtgtgaag ttagtggaac aactatgcca
     9481 gcgtctgcgc gtgccaaatg aaattcgcga tttagccaga ctggtggctg agtttcacga
     9541 tctcatccac accttcccaa tgctgaaccc aaaaaccatc gtcaaactat ttgattccat
     9601 cgacgcctgg cgtaaaccgc agcgtgtcga gcaactggcg ctgaccagcg aggctgatgt
     9661 gcgcggcaga accggttttg aatcagccga ctacccgcaa ggccgctggt tgcgcgaagc
     9721 ctgggaagtg gcacagtcag tgccgacaaa agccgtcgtt gaagcgggat ttaaaggtgt
     9781 ggagattcgc gaggagctga cccgacggcg gattgcggcg gtggctggct ggaaggaaca
     9841 gcgttgccca aaacctgagt gagtgttgga tattttgccg gatgcgacgt aagcgtctta
     9901 tccggctaca tgtcaacgac agttgtaggc ctgataagac gcgcgagcgt cgcatcaggc
     9961 gttgattgcc ggatgcggcg tgaacgcctt atccggccta cgcaatctga taagacgcgt
    10021 cagcatcata tccgacactg ttcttcacat caaaagaaca caacgtacac cgcagccgcc
    10081 acaataaagc gataaatggc gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag
    10141 gttttaatcg ctatcagcgc caccacaaaa gcggtgataa acccaacggc aaacatcggg
    10201 atatcgccgg tagtcaggaa gccccagctt ttgtagagat cgagcgccgt tgcgcccatc
    10261 atcatcggca ccgccagcag gaacgagaac tcggaagcag cgtaacggct cacccccatc
    10321 agcatcccac ctgaaatggt cgccccggaa cgggagaaac ccggccacag cgccagacac
    10381 tggaaacagc caatcataaa cgcctgacga taggtcatat catcaagacc cggcgcacgc
    10441 ggctctttcg gcttcaggca ttcggcggca atcagcaaca gaccgccaac gaccagtgca
    10501 tacatcacat ttatcgggtt aaacaatgat ttaatcgtgt cgtggaacaa cagccccagt
    10561 accaccgccg gaatcatccc cagcaaaatg tggatcagcg ttaaacggcc tttgctttct
    10621 ccttcgtgct gcaacgggcg accaaagtga ataccaataa ggccaaacag acgtcgccag
    10681 aacatgacaa ccactgccag aattgatcct aactggatca cgacttcaaa ggttttcgcc
    10741 gtgtcgccct caaaccccaa caagtgaccg acaataatca tatggcccgt gctggatacc
    10801 ggcagaaatt ctgtcaatcc ttcgaccaca cccaatattg ccgctatcag cagcgagtgc
    10861 atatcgctca tcaataaacc cctaaattat aaaaatgtaa cgcttgtccg aactactgca
    10921 gtatgaccag gttataaccg tttggtttaa cagctgtaaa attaattatt ttctttcaga
    10981 ttattgccac gctcaatgat tacgccaaca ttcgccgccc gcgccactgc gcctggcttg
    11041 ctgagtttga tacgcaccca cggcgagttg aagcgtgcta acagcaactc cgccacctct
    11101 tcagccacac gttccaccag cgcaaaacgc gccccctcga cgtggctgac taccgtttct
    11161 gcaatgtcag cgtaactgag gcaatccgcc acatcatcac ttttcgccgc tttacggtta
    11221 tcccacgcca tttcgatatc gaacactaac ttctgttcga tggtctgttc ccagtcgtaa
    11281 acaccaatag tggtgattac cgaaagttgc tctataaata caatatccat cacgtcctgc
    11341 ctgctttttg gctaaccgga taccacttcc ggcgaaatgt gcgtattatc cacagattca
    11401 tcgttgaaca cgaattttca aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat
    11461 cctcatcgcg tacctctgcg gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg
    11521 gctgcccgat ccgcgaacca gcggctccgg caatccaggc gcaaccaatg tgttacgtat
    11581 cggtggcaag ggagcagccg tagcagtact gattttcgac gttctgaaag gaatgttgcc
    11641 cgtctggggc gcgtatgaat taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc
    11701 cgcctgtctt ggacacatct ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc
    11761 taccgctttt ggtgccatcg cacccattgg ctgggatctc accggagtaa tggcgggaac
    11821 ctggttactg accgtgctat tgagcggata ttcgtcgctg ggggcgattg tcagtgcact
    11881 gattgctccg ttttatgtct ggtggtttaa gccacaattc accttcccgg tttcgatgct
    11941 ctcttgcctg atcctgctgc gccatcatga caacatccag cgtctgtggc gtcgtcagga
    12001 gacaaaaatc tggacgaaat tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt
    12061 ggagctacat aacctgatag ccttcgtcgg gcttgcccag ccgttgctgg caccatgccg
    12121 ccagaaattc cacgcagaca cgtaatttca tgctgcgata gagcggctcc cggtaaacag
    12181 cccagatatt ggcgctttgt gcatactccg gcaatactcg caccagtttg ccactctcca
    12241 gaaacggcaa cacatcccac tcggaacgca acataatccc tttgccctcc agcgcccatt
    12301 gcaacactat ctcgccgcta ttggaggaaa gatgcccgct taccttcacc gattttttct
    12361 cctgaccgtt ccccaactcc catattccgt gggtcatatc gcgctctttg gtcaccaggc
    12421 agtcatgacg acttaattcc tgtaaggatt gtggctgcgg atatttttgc agatactcag
    12481 gtgctgcgca taatattctt ttattttttg tcaaaagatg cgcaatataa taatcaggaa
    12541 ttgcgtcatt aatacgaata tccagatcaa tattatcctg cactaaatca atttgccgat
    12601 cgaacaattc aaaatgcacc tgtaactcag gataattgcg catcagttcg gtaatggctg
    12661 gagcaatatg gctgcgccca aaaccaaaac tgcaaccaat gcggatcatc ccctccgggc
    12721 gcgttttgat ttgcgtgacg tcatcgacca gtcgctgata ttgggtgagg atttccagcg
    12781 catgttcgta acaacgttgg ccactttcag tcagcgccac gccgcgagcg gagcggttca
    12841 gcagagttgt tgccagcgta ttttcaagga tttggatccg cttggtgaca aatgcaggcg
    12901 tttgcccaag cgtcgccgct gcagcactaa aactcccgct atggacgatt tccaccagta
    12961 cctgtaagtc tttggctaaa ggccagctat ttagcattgt taaatatccg cagttttgat
    13021 aaacgcagtg tatttcgcaa aacattacca ttccctgctc tggcgcaata ttcgttctta
    13081 ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc
    13141 acatttttag tggtagtttg tggcggtgaa tttttccaga caaatacata aactggagtt
    13201 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacgga gattgtcgct
    13261 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
    13321 ctaaaggatg ccgaaacatc gtcgatggga aaaatcatct accacacgat gttcgataac
    13381 atgcaaaaag cgatcgacct gaatcgtcct gcctgtcagg acaccggcga aatcatgttt
    13441 tttgttaagg tcggttcccg tttcccactg cttggcgagc tgcaaagcat cctcaaacaa
    13501 gccgtggaag aggcaaccgt caaagcgcca ctgcgtcaca atgcggtaga aatttttgac
    13561 gaagtgaaca ccggcaaaaa taccggcagc ggcgtaccgt gggtcacctg ggatatcgtc
    13621 cccgacggtg acgatgcgga aatcgaagtt tacatggcag gcggcggctg cacactacct
    13681 ggtcgttcga aagtgttaat gccgtcggaa ggatacgaag gcgtggtgaa attcgtcttc
    13741 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtagg cgtgggcatc
    13801 gccacctcgg tggaaaccgc cgctgtgctc tcacgtaaag ccattttacg cccaattggc
    13861 agccgccacc ccaatccgaa agcggcagag ctggagctac gcctggaaga aggactcaac
    13921 cgtctgggga ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtacatatc
    13981 gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg tttctacagg ctgctgggcg
    14041 catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
    14101 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
    14161 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc
    14221 accgtcgctt gatcgaactc aaacgtccga tcccttacga tctcaacggc aaagcgattt
    14281 tccacgctgg ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggta tccgtcggcc
    14341 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgaacag accggcgtga
    14401 aactggtggt cggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca
    14461 aggcgctgca tgtgattttc ccggcaggct gcgcagtact ggcggcaacc caggtggaag
    14521 agattgaaga agtgcactgg acagaactcg ggatgccgga gtcgctgtgg gtctgccggg
    14581 tcaaagagtt cggtccgctg attgtttcta ttgataccca cggcaacaac ctgatagccg
    14641 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatt tgcgagcacg
    14701 tccactacat caaataaccc tcccggagag gctcaccccc tctccttttt cgcaggcata
    14761 acacgatgaa accttccact gaatggtggc gataccttgc gccgctggcg gtcatcgcca
    14821 ttattgctct aattccggtt cccgcagggc tggagagtca tacctggctc tactttgccg
    14881 tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga
    14941 tggtgggtat ctccattatc gccattctct cgccgtggct gctgtttagc ccggagcagc
    15001 tcgcccagcc aggttttaag ttcaccgcca aatccctctc ctgggcggtt tctggttttt
    15061 ctaattcggt tatctggctg attttcgccg cctttatgtt tggcacaggc tatgaaaaaa
    15121 ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt
    15181 ttctcggcta tgcggtgatg ttctccgagc tgatcctggc acctgtaaca ccgtccaact
    15241 cggcgcgcgg tgcggggatt atctatccca tcatccgtaa cctgccaccg ctctatcaat
    15301 cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta catcatgtgg atggggattg
    15361 ttgccgactg cgtgaccagc gccattttcc tgacggcgat ggcacctaac ttgctgttaa
    15421 ttggcctgat gaaaagcgca tctcacgcca cactgagttg gggcgactgg ttcctcggga
    15481 tgttgccgct cagtatttta ctggttctgc tggttccctg gctggcttac gtgctgtacc
    15541 cgccggtact gaagtctggt gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa
    15601 tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct gatggtaggc gcgctggtgc
    15661 tgtggatttt cggcggtgat tatatcgatg ccgcgatggt cggttacagc gtggtggcgc
    15721 tgatgctgct tctgcgcatt atcagttggg acgacattgt cagtaataaa gccgcgtgga
    15781 acgttttctt ctggctggcc tcgcttatca ccctcgctac cggactcaac aacaccggtt
    15841 ttattagctg gtttggcaaa ctgttagcag gcagcttaag cggttattcg ccaacgatag
    15901 tgatggtggc gttgattgtg gtgttttatc tactgcgcta ctttttcgcc agcgccacgg
    15961 cgtatacctc cgctctcgcg ccgatgatga ttgccgccgc gctggcgatg ccggaaatcc
    16021 cgctgccggt gttctgcctg atggttggcg cggcaattgg tctggggagc attcttacgc
    16081 catacgccac cggacccagt ccgatttact acggtagtgg ttatctgcca acggtggatt
    16141 actggcgact gggggcaatt tttgggctga tattcctcgt gttgctggtg attaccggct
    16201 tactgtggat gcccgtggtg ttgctttaag tgaggacggt gccggaagtg actctccggc
    16261 acctaagagt tttacgcggc cggtaactct gccagcggcc agcgcggacg cacgctaacg
    16321 ccaagatccg ccgtcgcgcc tgctttaaac cgcaccattc cggcataggc gatcatcgcg
    16381 ccgttatcag tacaaaattc cggacgcgcg tagaacactt cgccacggcg ttttttcatc
    16441 atttccgcca gcttcgcccg taacgtgcgg ttagcactca cgccacctgc cattaccagt
    16501 cgcttaaagc ccgtctgatc cagcgctcgc ttacacttaa tcatcaacgt atcgaccacc
    16561 gcatcttcaa aggcgcgggc gatatcagca cgcgtctggt cgtcggtgcc gttatcacga
    16621 atggtatttg ccgcgaaggt tttcagacca gaaaagctga aatccagccc tggacggtcg
    16681 gtcatcgggc gcgggaagac aaagcgcccg gctgtaccct gagccgccat tttcgacagt
    16741 aatggcccgc caggataatc cagccccagc agcttcgcgg ttttatcaaa cgcttcacca
    16801 gccgcatcat cgatagactc gccgagcagc tcgtactgac caatgccagt cacgctgatt
    16861 aactgcgtat gaccgccgga aaccagcagc gccacaaacg gaaattccgg tgggttatct
    16921 tccagcatcg gcgctaacag atgcccttcc atatggtgta cagggatcgc cggaacgttc
    16981 caggcaaacg ccagagaacg ccccacggtc gcgccaacca gcagtgcgcc gactaatcca
    17041 gggcctgcgg tataggccac agcatcaatg tcttttgccg ttaaaccaga ctcctttagc
    17101 gccgcctgga tcaacggtac ggttttacgc acatgatcgc gggaggccag ctcaggcact
    17161 acgccgccgt agtcagcgtg caatttcacc tgactataca attggttggc taacaaacct
    17221 ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg aagtttcaat acccagtaca
    17281 cgcatgactt gttttacctc gctttattac cgcgcagtgt aggaccaatg cgggttgatg
    17341 taaaactttg ttcgcccctg gagaaagcct cgtgtatact cctcaccctt ataaaagtcc
    17401 ctttcaaaaa aggccgcggt gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc
    17461 attttgaaat aagctggcgt tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc
    17521 acatgccggt aattaaagta cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca
    17581 agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa
    17641 aaccgactac cgaacgtaag cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac
    17701 tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt ccccgagagc gttgctctcc
    17761 gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc
    17821 gtttatgaat tgctaaaaat cggggcctat ggctggacga atcccacgcg tattcattaa
    17881 tgatctgctg gcacgcactg acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa
    17941 gcagggcaag aatttccacg cgtgttgtcc attccacaac gagaaaaccc cgtcattcac
    18001 cgttaacggt gagaaacagt tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat
    18061 cgacttcctg atgaactacg acaagcttga gttcgtcgaa acggtcgaag agctggcggc
    18121 aatgcacaat cttgaagtgc catttgaagc aggtagcggc cccagccaga tcgagcgcca
    18181 tcaacgacaa acgctttatc agttgatgga cggtctgaat acgttttacc aacaatcttt
    18241 acagcaacct gttgccacgt ctgcgcgcca gtatctggaa aaacgcggat taagccacga
    18301 ggttatcgcc cgctttgcga ttggttttgc cccccctggc tgggacaacg tcctgaagcg
    18361 gtttggcggc aatccagaaa atcgccagtc attgattgat gcgggcatgt tggtcactaa
    18421 cgatcagggg cgcagttacg accgtttccg cgagcgggtg atgttcccca ttcgcgataa
    18481 acgcggtcgg gtgattggtt ttggcgggcg tgtgctgggc aacgatacac ccaaatacct
    18541 gaactcgccg gaaacggaca ttttccataa aggccgccag ctttacggtc tttatgaagc
    18601 gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc gaaggctata tggacgtggt
    18661 ggcgctggcg caatacggca ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc
    18721 cgatcacata caactgttgt tccgcgcgac caacaatgtc atttgctgtt atgacggcga
    18781 ccgtgcaggc cgcgatgccg cctggcgagc gctggaaacg gcgctgcctt acatgacaga
    18841 cggtcgtcag ctacgcttta tgtttttgcc tgatggcgaa gaccctgaca cgctggtacg
    18901 aaaagaaggt aaacaagcgt ttgaagcgcg gatggagcag gcgatgccac tctccgcatt
    18961 tctgtttaac agtctgatgc cgcaagttga tctgagtacc cctgacgggc gcgcacgttt
    19021 gagtacgctg gcactgccac tgatatcgca agtgccgggc gaaacgctgc gaatatatct
    19081 tcgtcaggaa ttaggcaaca aattaggcat acttgatgac aaccagcttg aacgattaat
    19141 gccaaaagcg gcagagagcg gcgtttctcg ccctgttcca cagctaaaac gcacgaccat
    19201 gcgtatactt atagggttgc tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct
    19261 tgagaatctg gatgaaaata agctccctgg acttggctta ttcagagaac tggtcaacac
    19321 ttgtctctct cagccaggtc tgaccaccgg gcaactttta gagcactatc gtggtacaaa
    19381 taatgctgcc acccttgaaa aactgtcgat gtgggacgat atagcagata agaatattgc
    19441 tgagcaaacc ttcaccgact cactcaacca tatgtttgat tcgctgcttg aactgcgcca
    19501 ggaagagtta atcgctcgtg agcgcacgca tggtttaagc aacgaagaac gcctggagct
    19561 ctggacatta aaccaggagc tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg
    19621 atcgggaagc ccccgacagc cgcactgaga ggcagcggca aatatataag tacgccctcg
    19681 taattatcgt tggcggtaaa caaccgttgg atttcagcgt taacggctga aggacatcgg
    19741 gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat accgtcttat ggagcaaaac
    19801 ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg agcaaggcta tctgacctat
    19861 gccgaggtca atgaccatct gccggaagat atcgtcgatt cagatcagat cgaagacatc
    19921 atccaaatga tcaacgacat gggcattcag gtgatggaag aagcaccgga tgccgatgat
    19981 ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt
    20041 tccagcgtgg aatctgaaat cgggcgcacg actgacccgg tacgcatgta catgcgtgaa
    20101 atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa
    20161 gacgggatca accaggttca atgctccgtt gctgaatatc cggaagcgat cacctatctg
    20221 ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc
    20281 tttgttgacc cgaacgcaga agaagatctg gcacctaccg ccactcacgt cggttctgag
    20341 ctttcccagg aagatctgga cgatgacgaa gatgaagacg aagaagatgg cgatgacgac
    20401 agcgccgatg atgacaacag catcgacccg gaactggctc gcgaaaaatt tgcggaactg
    20461 cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc
    20521 gctcaggaag agatcctgaa actgtctgaa gtattcaaac agttccgcct ggtgccgaag
    20581 cagtttgact acctggtcaa cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa
    20641 cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc
    20701 ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg cggcaattgc aatgaacaag
    20761 ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc atcgcgccct gcagaaactg
    20821 cagcagattg aagaagaaac cggcctgacc atcgagcagg ttaaagatat caaccgtcgt
    20881 atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag agatggttga agcgaactta
    20941 cgtctggtta tttctatcgc caagaaatac accaaccgtg gcttgcagtt ccttgacctg
    21001 attcaggaag gcaacatcgg tctgatgaaa gcggttgata aattcgaata ccgccgtggt
    21061 tacaagttct ccacctacgc aacctggtgg atccgtcagg cgatcacccg ctctatcgcg
    21121 gatcaggcgc gcaccatccg tattccggtg catatgattg agactatcaa caaactcaac
    21181 cgtatttctc gccagatgct gcaagagatg ggccgtgaac caacgccgga agaactggct
    21241 gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc tgaagatcgc caaagagcca
    21301 atctccatgg aaacgccgat cggtgatgat gaagattcgc atctggggga tttcatcgag
    21361 gataccaccc tcgagctgcc gctggattct gcgaccaccg aaagcctgcg tgcggcaacg
    21421 cacgacgtgc tggctggcct gaccgcgcgt gaagcgaaag ttctgcgtat gcgtttcggt
    21481 atcgatatga acaccgacca cacgctggaa gaagtgggta aacagttcga cgttacccgc
    21541 gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct
    21601 gaagtgctgc gtagcttcct ggacgattaa tcggtaggcc ggatcaggtg ttacgccgca
    21661 cccggcacta gtccctccgc acaaacgcca ccttttcggt ggcgttttta tcgcccacgc
    21721 accaccagtg cctggtccag ctcgcgatac gcttcaacca gtttctccag tgaaacgcga
    21781 cttaaaccgc tgggatttgg cagcacccaa atctgcgtcg aaccaatggt gagagtttgt
    21841 ttcccccact gtgcaccgcg ctggctgaat ccctgttcat atgcttgttt gcccagaatc
    21901 gccaacgcct gcggctgata atcttcaatt ttttcaatca gcttacgccc gcctgcgtgt
    21961 agctcctgct ttgaaacttc attggcttgc accgttggac ggtctaccag tttggtgacg
    22021 ccacaacgat aatccagcag atgctgtgcc tcctgcggct tcaactgacg gtcggtaaac
    22081 ccggcctgat atatcacctt ccagaagcga tttgccggat gagcaaaggg aaaaccagtc
    22141 ccggcggatg aaagcccagg gttgataccg caaaacacga cccgtaaccc tggagccaaa
    22201 atatcctcaa ccatatttac tcctttctaa catcaccagg aaaagtataa cggattgaaa
    22261 atacattgtt tataaaaaca gcaggcgcgc ggtaatggct ggattgcgac acggagttac
    22321 tttataatcc gctaccatgg ccccttagct cagtggttag agcaggcgac tcataatcgc
    22381 ttggtcgctg gttcaagtcc agcaggggcc accagatata gcaaaggctg acgagaaatc
    22441 gtcagccttt ttctttttat atatcagtta ctttgcgtgc cagtaagccg ctgcacgtac
    22501 ccgctgtggg tcatactgtt ccgcttcaaa gcggcggctt aaattcttaa cgactttgcc
    22561 ttcgccggtt atccagatga agtaatcatc ggcagggatt tgcatctgcg ccagacgcgc
    22621 atctaccgcc tgctcatcat gcgccagcca ttcgatatta aaaccatcaa ggtgcgcgag
    22681 ataatcctga caggcgttat cccgcacgct aaccagcgca ctaacttgcg gtttaacggc
    22741 aagtttgctc aacgtttcca ggcggcggcg cagtgcgggc attccggatt catcgcagac
    22801 atacagctga tacgcgtaat cctctggcac caccagcgaa ccgcgcggac ctgccaccgt
    22861 gagtttatcg cccggttgcg cctgcattgc ccagctgctg gcgacgccac cttcgtgaat
    22921 aaagaaatca atcgccagtt catggcgtag ttcgtcatac agcggcgtat agtcacgcga
    22981 cggtgggcgt ggtccttccg gccagacgat gccctcttcc gttaccgttg gcggcacaaa
    23041 gtgagcatca gattgaggaa agaagagttt gctgtgatcg tcaaagccgc gcgatgtaaa
    23101 accgtccagc gcctcgccgc caaggacaat gcgctgaaaa ccgacgctaa tacgctcaac
    23161 gcgtagcaca gtcaattcac ggaagcgcag atcattgcga acgcgctgtg ggtagcgggg
    23221 ggtgttattc attgttatcg ccttcatgat ggtaatcaga tatatctaaa taaaatttcg
    23281 caaatgataa tgattgttaa tcatgataaa tgcaagcgat ttgtagaact gatatgtcta
    23341 tagtctgata agacgaaccg cctcttctca ggcatcatta ctcaacgccg gatgcggcgt
    23401 gaacgcctta tccggcctac gtgtgagatg agtccacgtt taaaaatcat tattgtactt
    23461 gcagtaatgc tcatatcaga tataaattag atatatctaa ttaaacaaaa ggaggctgat
    23521 atgagccatc atcacgaagg gtgctgtaaa catgaaggcc agccacgcca tgagggctgc
    23581 tgcaaaggtg agaagtcaga acacgagcac tgcggacacg gtcaccagca tgaacacggt
    23641 caatgctgcg gtggtcgcca cggtcgcggc ggcggtcgtc gacaacgttt ctttggtcac
    23701 ggtgaattgc gtctggtgat tctggatatt ctctcgcgcg atgacagcca cggttacgag
    23761 ttgattaaag cgattgaaaa tctgacccag gggaattaca ccccaagccc gggcgtcatc
    23821 tacccaacgc tggattttct gcaggagcag tcgctgatta ccattcgcga agaggaaggt
    23881 ggtaagaagc agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga aaaccgcgaa
    23941 caggtagaga tgattgaaga acgcatcaaa gcgcgttgcg ttggcgctgc gctgcgccag
    24001 aacccgcaaa tgaagcgggc gctggataat tttaaagcgg tactggattt acgcgtcaac
    24061 cagagcgata tcactgatgc acaaataaaa aagatcattg cggtgatcga ccgcgccgct
    24121 tttgatatta cgcaactgga ttaatcaccg attgccggat gtggcgtgcc ctacccccgt
    24181 aggcctgata agatgcgcca gcatcgcatc aggcattgtg cgccaaccgc cggatgcggc
    24241 atatcgatta atgcagcacc gtcaccgcgt cttccagtcg gctggcgcgg tgtttcacca
    24301 tcgccgacac ctgcgcactc tcttccacta actcggcgtt tttctgggtg atcaggttaa
    24361 gctcatccac tgcacgggtc aggctggaaa gcccatcggc ctgttccagc gttgaatggc
    24421 taatctgagc tatcaactgg gtgacgtttt ttacctgtgc cacaatatct tccatcgtcc
    24481 gtccggcggc gtgtacctgc tgcgaaccgg attgcacctt atcagcactg gcatcaatca
    24541 gcttgcgaat atcgttggca gcattggcgc tgcggctagc taaatgacgc acttcccccg
    24601 ccaccactgc aaaacctttg ccctgttcgc cggcacgcgc cgcttccacc gccgcattca
    24661 gggccagaat attggtctga aacgcaatat cgttaatcag cgaagtaatg gtgccaatgc
    24721 gctgggtact gtcggcaata tcgtccattg tcttgatcac cgtagtcatc gcctccccac
    24781 cctgcactgc cgcattactg gcagtgatag aaagtttatc ggcagccgac gccgtggcag
    24841 agttctgttt caccgacgcc gccatttggt tcatggtcgc caccgtttgc tgaacgttat
    24901 caactgtctg ctgggtatgt tcgttcagtt catcggtgcc tttcgccagc gtctcactgc
    24961 cgtttctgac actggacacc tggcttgaga catcgttaat tagccaacgg cacatcaggc
    25021 caagctgccc taccgcgcgt aatgtcagcc ccagctcatc gctgcgattc agatgctcaa
    25081 cactattacg ctctccggtc gccaccttca gtgcctgacg ggcgacattt tctatcgggc
    25141 ggacaatctg ccattcaaaa caagcgcttg ccaataatac cactaacgca cagaggatat
    25201 acgtcaccac cggggcagca acaaaccata gcatggccgt cagcaagata aacatcaggg
    25261 tcatcacgcc acgcgcccgc cagcgaagcg gtaatgaagg cagtttaccc agccatcctt
    25321 tacgcaccac caggccttta tgaatacgct tactggtacg tccggcgttc aacgctttgt
    25381 acagcggctc caccgccgcg atctcttcat ccgtcgcccg ggtacgaatc gacatatagc
    25441 cactgatttt tccctcgcgc accatcggta ccgcattggc ccgcacccaa taatggtcgc
    25501 catttttgcg gcgatttttc acgatgccgc tccagggctc cccttttttc agggtgaacc
    25561 acatatccgc aaacgccgct tttggcatat ccgggtgacg caccatgttg tgcggttgac
    25621 cttgtaactc ttgcaaggta tagccgctca cctgcacaaa agtgtcatta gcatgagtga
    25681 tatagctttg cagatcggta gtggacatca gagtggtatc gtccgccagc ggggtatttt
    25741 gctgggtgac atacggatga gaagacatgt tcgcgtcctg tgcaggttat atggttgtta
    25801 acttcttgtc agagtttatg tcggccccgc tgcggttatc tttaactgat taatttgatt
    25861 tagatcgcaa tttgcgatta aagcataaat ctaatccctt gattt
//
LOCUS       DAZGAP010000067        25791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGAP010000067 DAZGAP010000000
VERSION     DAZGAP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.067"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            300..1598
                     /gene="kgtP"
                     /locus_tag="ACHLAM_002274"
     CDS             300..1598
                     /gene="kgtP"
                     /locus_tag="ACHLAM_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO7853792.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1595..1918)
                     /gene="yfiM"
                     /locus_tag="ACHLAM_002275"
     CDS             complement(1595..1918)
                     /gene="yfiM"
                     /locus_tag="ACHLAM_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO7853793.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1962..3317)
                     /gene="pssA"
                     /locus_tag="ACHLAM_002276"
     CDS             complement(1962..3317)
                     /gene="pssA"
                     /locus_tag="ACHLAM_002276"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO7853794.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3431..6091)
                     /gene="pat"
                     /locus_tag="ACHLAM_002277"
     CDS             complement(3431..6091)
                     /gene="pat"
                     /locus_tag="ACHLAM_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO7853795.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6123..6821)
                     /gene="tapT"
                     /locus_tag="ACHLAM_002278"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6123..6821)
                     /gene="tapT"
                     /locus_tag="ACHLAM_002278"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO7853796.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6890..7309)
                     /gene="trxC"
                     /locus_tag="ACHLAM_002279"
     CDS             complement(6890..7309)
                     /gene="trxC"
                     /locus_tag="ACHLAM_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO7853797.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7516..8553
                     /gene="yfiF"
                     /locus_tag="ACHLAM_002280"
     CDS             7516..8553
                     /gene="yfiF"
                     /locus_tag="ACHLAM_002280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7853798.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8601..9290)
                     /gene="ung"
                     /locus_tag="ACHLAM_002281"
     CDS             complement(8601..9290)
                     /gene="ung"
                     /locus_tag="ACHLAM_002281"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO7853799.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREDVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            9595..9978
                     /gene="grcA"
                     /locus_tag="ACHLAM_002282"
     CDS             9595..9978
                     /gene="grcA"
                     /locus_tag="ACHLAM_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO7853800.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10034..10621)
                     /gene="eamB"
                     /locus_tag="ACHLAM_002283"
     CDS             complement(10034..10621)
                     /gene="eamB"
                     /locus_tag="ACHLAM_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO7853801.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10724..11605
                     /gene="yfiE"
                     /locus_tag="ACHLAM_002284"
     CDS             10724..11605
                     /gene="yfiE"
                     /locus_tag="ACHLAM_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7853802.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11638..12972)
                     /gene="srmB"
                     /locus_tag="ACHLAM_002285"
     CDS             complement(11638..12972)
                     /gene="srmB"
                     /locus_tag="ACHLAM_002285"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO7853803.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13104..13841
                     /gene="trmN"
                     /locus_tag="ACHLAM_002286"
     CDS             13104..13841
                     /gene="trmN"
                     /locus_tag="ACHLAM_002286"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO7853804.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13826..15448)
                     /gene="nadB"
                     /locus_tag="ACHLAM_002287"
     CDS             complement(13826..15448)
                     /gene="nadB"
                     /locus_tag="ACHLAM_002287"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO7853805.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15704..15859
                     /gene="rseD"
                     /locus_tag="ACHLAM_002288"
     CDS             15704..15859
                     /gene="rseD"
                     /locus_tag="ACHLAM_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO7853806.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15856..16431
                     /gene="rpoE"
                     /locus_tag="ACHLAM_002289"
     CDS             15856..16431
                     /gene="rpoE"
                     /locus_tag="ACHLAM_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO7853807.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16464..17114
                     /gene="rseA"
                     /locus_tag="ACHLAM_002290"
     CDS             16464..17114
                     /gene="rseA"
                     /locus_tag="ACHLAM_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO7853808.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17114..18070
                     /gene="rseB"
                     /locus_tag="ACHLAM_002291"
     CDS             17114..18070
                     /gene="rseB"
                     /locus_tag="ACHLAM_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO7853809.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18067..18546
                     /gene="rseC"
                     /locus_tag="ACHLAM_002292"
     CDS             18067..18546
                     /gene="rseC"
                     /locus_tag="ACHLAM_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO7853810.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18744..20543
                     /gene="lepA"
                     /locus_tag="ACHLAM_002293"
     CDS             18744..20543
                     /gene="lepA"
                     /locus_tag="ACHLAM_002293"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO7853811.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20559..21533
                     /gene="lepB"
                     /locus_tag="ACHLAM_002294"
     CDS             20559..21533
                     /gene="lepB"
                     /locus_tag="ACHLAM_002294"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO7853812.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21805..22485
                     /gene="rnc"
                     /locus_tag="ACHLAM_002295"
     CDS             21805..22485
                     /gene="rnc"
                     /locus_tag="ACHLAM_002295"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO7853813.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22482..23387
                     /gene="era"
                     /locus_tag="ACHLAM_002296"
     CDS             22482..23387
                     /gene="era"
                     /locus_tag="ACHLAM_002296"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO7853814.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23399..24127
                     /gene="recO"
                     /locus_tag="ACHLAM_002297"
     CDS             23399..24127
                     /gene="recO"
                     /locus_tag="ACHLAM_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO7853815.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGESVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24139..24870
                     /gene="pdxJ"
                     /locus_tag="ACHLAM_002298"
     CDS             24139..24870
                     /gene="pdxJ"
                     /locus_tag="ACHLAM_002298"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO7853816.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIPVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24870..25250
                     /gene="acpS"
                     /locus_tag="ACHLAM_002299"
     CDS             24870..25250
                     /gene="acpS"
                     /locus_tag="ACHLAM_002299"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO7853817.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25672..25752
                     /gene="shoB"
                     /locus_tag="ACHLAM_002300"
     CDS             25672..25752
                     /gene="shoB"
                     /locus_tag="ACHLAM_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO7853818.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
BASE COUNT         6317 a         6420 c         6809 g         6245 t
ORIGIN      
        1 caggatggcc tttttgcgct ggagcagaat aaatgccgga tgcgacgctg gcgcgtctta
       61 tactcccaca taagctagat tcagcagcga atacggcttc cccaacgtgc ccacctccat
      121 acgtgtcctc cttaccagaa atttatcctt aagctcctca ataaccattt tcctgctaac
      181 taaattcgtg gttaaggttg cataatgata tgcaacaaat gtataacatt ttctttacca
      241 atagaaataa acaaaagcga ccgacaaaag catcggatta cggcaggaga cataatggca
      301 tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgatact cgtcgccgca
      361 tttgggcaat tgtgggggcc tcttcaggta atctggtcga gtggttcgat ttctatgtct
      421 actcgttctg ttcactctac ttcgcccaca tcttcttccc ttccgggaac acgacgactc
      481 aactactgca aacagcaggt gtttttgctg cgggattcct gatgcgccca ataggcggtt
      541 ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgctg ttatcggtgt
      601 gtatgatgtg tttcggatcg ctggttatcg cctgcctccc aggttatgaa actataggta
      661 cgtgggctcc ggcattattg cttctcgctc gtttatttca gggattatcc gttggcggag
      721 aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc aaaggttttt
      781 acgcatcatt tcagtatgtg acgttgatcg gcgggcaact gctagctcta ctggttgtcg
      841 tggttttaca acacaccatg gaagacgctg cacttagaga gtggggatgg cgtattcctt
      901 tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcag ttagatgaaa
      961 cttcacaaca agaaacacgc gctttaaaag aagctggatc tctgaaagga ttatggcgca
     1021 atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt tgtttctata
     1081 ccttcaccac ttatatgcag aagtatctgg taaatactgc gggaatgcat gccaacgtgg
     1141 cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca cttattggcg
     1201 cgctgtcgga taagattggt cgtcgtacct caatgttatg tttcggttct ctggcagcca
     1261 tttttaccgt tcctattctc tcagcattgc agaacgtttc ctcgccttat gccgcttttg
     1321 gtctggtgat gtgcgccctg ctgatagtga gtttttatac ttcaatcagt ggaatactga
     1381 aggctgagat gttcccggca caggttcgcg cattaggcgt tggtctgtca tatgcggtcg
     1441 ccaatgctat atttggtggg tcggcggagt acgtagcgtt gtcgctgaaa tcaataggaa
     1501 tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcattt ctggtttcac
     1561 taacattgca ccgtaaagga aaagggatgc ggctttagtg acgggtcagt tgccagacgg
     1621 tatagccggt gcttgcaccg gcgatatccc aggcaaaatc cttccagctc cagccgctcc
     1681 cctcagggcg gctatcccaa agctcttttg acgcccccag actgacagag aacatcaatc
     1741 caaacatggc actgcgatcc cggctcatcc cctgatgctg tgcatattca tttccggcgg
     1801 cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc cagctatcgt
     1861 tagccatatg gctgcatcca gaaagtaaca atagcgaaca gacaaaaaga atacgcaaaa
     1921 cgtggcctcc aaaacaaacc ccgtacagga cggggttgtg attacaggat gcggctaatt
     1981 aatcggtcga tgcggatacg gcgcaaacga cggatgagtt tacgaacctt caccggataa
     2041 tcggcaatac tttgcagatc gcgatagtgc ttaacgatgg tggtatgctc gcggatcagc
     2101 tccagttctt tctctcgctg tggcgcaagc tccagttgcg gatcgtggat caaaatggcg
     2161 ttttccagat ccagacgcca ggcgcgcggg ttcaggttat taccggtgat caacatccac
     2221 ttatcatcaa cccacatccc ttttaggtga taggtgttgt cgtcatcttt ccataaccga
     2281 accactagct ggtcagtatt gacgtaatac tgcaaacggc tcaggaaacg acgcagattg
     2341 atctcataga gataaggcaa tgcgccaatt atcttgaaag gttcatcttc cggaatgtag
     2401 aagtcgttcg cggttttatc accaacaata atttcgacct ttttcccttc gcgcagcaac
     2461 tggataatat tgcgcacaag gattgctggc aggttgaagt atggcgtaca gatggttagt
     2521 ttctgctcgg cacaaggcat aagatggaaa atggtcttgt tcaacagact cgatttcccc
     2581 agccccacta gcggcgttac ggaaagctga tcgttgtcgg catcgccctg gaaatgataa
     2641 gcggcatcac gcagctcctg gcggaacagg cgaatgtcgt tcttgatttc cgggcttttt
     2701 ggccgattaa catcatccag acgattaacg ccgcggccat tcataatatt ctgtgtaacc
     2761 cattcaaaca taatgtctga catcttacgg ttacggatta gatgataacg gtcgtagcga
     2821 tatttatcgt gctgatgcag gtaaacatcg ttcaggctgg caccgctata aagtacgcta
     2881 tcgtcgatga taaagccttt aaagtgcaga acaccaaggg cttcacgggt attgattgga
     2941 acgccataaa ccggaacatc tacgcccgga ttttcctgcg ccatgcggca gtaccagtca
     3001 gcgttagtgt tagatgccgc agcgccaatg cgtccacgtt gtgcacgatg ccagtcgacc
     3061 agcacccgca catccagttc cggacgctgc cttttagcct catacaacgc gttcagaatg
     3121 cctttgccac cgtcatcctg ttcgagatac agggcaacaa tgcaaatgcg ctgcttcgcg
     3181 ctggctattt tttccagcaa cgtctcccgg aagtcggcgg gagcgtaaaa gaaatcgaca
     3241 tcatcaactg attgagaaat cttgggtagt tgggcaaggt gttgttgatg tttattacgc
     3301 ttaaattttg acaacatcac agtgcatttc ttctctgttc attgaagggt cctctgtgca
     3361 atgcagacga cataagcggg caataataac accagtcccg attaagtggt caacatttcc
     3421 agtaccttac tcatgattcc tcgcgctggg caagatttag cgtaagccca acgatcccct
     3481 cttcgagctg gatatcaacg ttaaacccga gcttgcgggc cagcgccacc atgccacgat
     3541 tgtttggcat cgtaatacca ttcagacgtt gtagtccgtg atctcgcgta taggtaatca
     3601 acttttccat taagcgtcga cctaagccta accctttgag atccgagcga accagcacgg
     3661 caaattcggc atcgatgtta tcagggtcgg agatcgcacg cgtgacgccg aggatctctt
     3721 ctgtttgatc aatacgtcgt accgccacaa aagccatttc ccgatcgtag tcgatctgcg
     3781 tcatgttggc taaatcttca tgggtaaatt cgttgatctc gctaaagtag cggtaataaa
     3841 gatcttcttt ggtgactcgc gaaatgaatt gctgaagctg tggctcatct tctggcaaaa
     3901 tcgggcggaa caagcagcgt tcaccgtttt tcaattctac ccattcttcc agctgatgcg
     3961 gataagggcg cactgccagc cgactctcgt tatcgccttc aaacggcgcg atatccagcg
     4021 tgacatccag cgcggtaaat tcactgccag aagccagcaa aggatgaata tccagacgct
     4081 gaatttccgg gcaatcgaca atcaagttag aaacctgcac cagaagctgg ctcaagcctg
     4141 caacatctaa tgggcgtagc gcactgcgcg cacgaatctt tttacttttg atcccctgaa
     4201 taaccagata gcgggccagg ttcatgttca gcggcggtag tgcgacgacg gcttgatctt
     4261 caggacgcca ctccacaccg ccttcgccca gcatgatcaa cggcccgaaa accggatcgt
     4321 gctcaaccac aacccgcaac tcctgagcgc cagcacggtt agccatactt tgcaccaaca
     4381 ggccgtggat ccgcgcctgc ggccaggcca tttttacgcg atcgaaaata gcgtttgccg
     4441 cttgctggac ttcattagct gtacgcaggt agagcattac gccctgaact tccgatttat
     4501 gtggaatatc cggcgaacgc aatttcagcg ccaccggata accaatctgt tcggcaatat
     4561 gcaccgcttc ggtgctatcg ctggcaatcc aggtggggag cgtgttcatg ccatacgctt
     4621 gcaggatggg ctgaacttca tgggtatcga gcgacgtagc cccttcggca atcgcctgtt
     4681 gcaacagaag atgcgcttct gcggtattag aggtcagatt gctgggcaac gccggcgttt
     4741 cgcgtagttg cttctgatta cgtcggtact ccaccatatg cataaaagca gtgatggttc
     4801 cttccggcgt gcgataggtc ggcagcccgg cttcactgaa taatcgtcgt gcctcttgcg
     4861 aggagtgctc gccgcaccag ttcgtcagca gagaaacata cttgctgcgg ggatgatgct
     4921 ttaccgcttc aattaatgct tgcgcacttt ctgttgcggg agcagcggcg ctgggcgaat
     4981 gaataaccat cagcgcgtca aaatcctggc tgtggagcag aatatccagc gttttaatat
     5041 agtgctcact gctggcgtca tcgcgtagat cgagcgggtt agagattgcc acatgttctg
     5101 gcagtgcatc gcgcagtttc tggcaggttt cttcgcttag cgttgccagc ttgccattgc
     5161 gtgaccataa ggcatccagc gccagcgcgg caggcgcagc accgttgctg ataatcatca
     5221 gccggtcgcc acgcagcggg cgcatatggc tgagggtttc caccgccgaa aacagctcgt
     5281 gagtatcctg tacccgcaac aaaccggcac gctgaatagc cgcatcccac gccggatcca
     5341 ttcctgccgt ggtgttcaac agacgttgag ccgccgggct acgtccgctt ttaatcacca
     5401 gaatcggttt gttgcgagag gcactacggg ccgccgaaac aaagcgtcgt gcgtcgctta
     5461 actgttcgag atagagcagg atggcgctgg ttttactgtc gcgggccaga tagtcgagca
     5521 attcatcaac gtcgatatcc aggctgtcgc cgagcgcaat aaagtaggaa aagcccatct
     5581 cacgctgttg cgcccagtcg aggatggtat tggagacggc agccgattgc gaaataaacg
     5641 ccagcttgcc gcgtttaatc ggcacaggcg aaaagctggc attcagacct tgccagggag
     5701 ccagtaaccc cagactgttt ggtcctagca ggcgcatgtt atggcgcagg gcgcaggcgc
     5761 ggagatcttc atgttgcgat gccggggcgg aaagaataat gcaggtttta cagcctttct
     5821 cgccgagctc ttccagaaga gcaagattac ggctggcatt ggtacataaa accgcaaggt
     5881 cgggtgtaaa gggcaagctg gcaatatccg gccaggccaa cacacccaac accgctttcc
     5941 aggctggcgt caccgggagt accggtccgt taaagcctcc cgccagcagg ttacgcatca
     6001 tcaggtaacc tgctcgattg gatttcatcg atgcgccaat taccgcaatc gattttggtc
     6061 gcagtagtgc ttccagtcct cgctgactca taccggtctc ccgcagaagt gaccgaatga
     6121 ttttaaacgc tttctaactg ttctgctgtg atgctaccca gatgttgcgt ttttcctgcc
     6181 agatagcgcg ttttaaaacg agtgaaatgc tcgcctaagc ccgttgcggc ttcggtatcg
     6241 ccggccatat ctaacagtgc gatggctacc tcggcagtac aatattggcc ttcagcctgg
     6301 gcttcacgca ggcgataggc agaaagccgg gaaagatcga cggaaatgac gggaagatta
     6361 tccagatacg gacttttacg aaacatcttg cgagcttcag gccaggtacc atcgagcatg
     6421 ataaatagcg gaggcttacc ggcaggtggt gtgaagatca cttcccgttg ctcatcagca
     6481 tacgaggcgg gaaagaccac cattggctga tagtccgggt tttgtaccag atccagcaaa
     6541 tctagcgagg gttcggtacg cgaccattga aacgcaacgg tatcaggcaa aatatcagca
     6601 atcagacgcc cggtattact gggcttcatt ggctcggtgt cgaacatcag caaacagaag
     6661 cgactttttg cttgtgctgg ggtaattgtc gaacagagac ataatgtctc tggcaaaaga
     6721 cagcgttggc agcgacgaac gcgattaccg cgggcaagaa aaggacgtgt tgcgcgcgca
     6781 atacgctcgg cgcgtaactg gagaacagcg ttttcggtca taagagagcg tcgaaaaaac
     6841 gccattgtcg cagaggagaa aacggggcac aagatgcgcc ccggtaagat taaagagatt
     6901 cgttcagcca gctatcaaac ggcgctttcg gtactgcgcc attaagcatg tcgacaacct
     6961 gaccgttttt gaaaatcatg atcgtcggta tactacgaat tccaaagcga ctgctcaatt
     7021 cacgttcagc ttcggtattc actttcacaa agcgcacttt accgctacgc tcttgcgcga
     7081 catcttcaaa aattggtgcg aaattacggc aggggccgca ccacggtgcc cagaagtcga
     7141 tcaccacagg tagatcatct ttcagcaatt tgtcgagcgt ttcaccggtc gcattaatca
     7201 cctctccgtc aaacaagtcg tgaccgcagc gtccgcattt tgccgcatct tcgatccgat
     7261 cgtcgggaat gcgattgatg gcctgacaat gggtacaaac ggtattcata actaacctcg
     7321 ggatgagtaa ggagacttat cggcaacgac gccggtatgt ttctaatatg ttacatatta
     7381 tcggggacat tgttccagac aacaatctgt tttattcaat aggtatgata gtcgcaggct
     7441 ttggtacgaa atcatggcga accgcgcgga cttcgggtaa tctgcgcgct tcgcgcagcg
     7501 ctggtggaga aaagcatgaa cgatgaaatg aaaggtaaaa gcggcaaggt caaagtgatg
     7561 tatgtccgca gtgatgatga ttctgataaa cgtacccaca acccgcgtac cgggaaaggg
     7621 ggcgggcgtc caggaaaatc tcgtgctgac ggtggccgtc gccccgcccg cgatgacaaa
     7681 cagagtcagc cccgtgaccg caagtgggaa gagtcgccgt ggcgcacagt ttcccgcgcg
     7741 ccgggtgatg agacgccgga aaaggccgat cacggtggca tcagtggtaa aagttttatt
     7801 gatccggaag tgttgcgtcg tcagcgtgcg gaagaaaccc gcgtctacgg cgaaaatgcc
     7861 tgtcaggcac tgttccagag ccgtccggaa gcgattgttc gcgcctggtt tatccagagt
     7921 gtaacgccgc gttttaaaga agccttgcgc tggatggcag caaaccgcaa agcgtaccat
     7981 gtggtagatg aagcggaact gacaaaagcg tcaggcacgg aacatcacgg cggcgtttgc
     8041 ttcctgatca aaaagcgtaa cggtacaacc gtgctgcagt gggtaagcca ggcaggcgcg
     8101 caggattgtg tactggcgct ggaaaacgaa tctaacccgc ataacctggg tggcatgatg
     8161 cgcagctgcg cgcacttcgg cgtgaaaggc gttgtggtgc aggatgcggc actgctggag
     8221 tcgggggcgg ctatccgtac cgcagaaggc ggcgcagagc acgttcagcc gattactggc
     8281 gacaacattg ttaacgtgct ggatgatttc cgtcaggcag gttacaccgt agtgaccact
     8341 tccagcgagc aggggaaacc gctgttcaaa accagtctgc cagcgaaaat ggtactggtg
     8401 ctgggtcagg aatatgaagg gttaccggat gccgcacgcg atccgaacga tttgcgcgtg
     8461 aagattgatg gtactggcaa cgttgccggg ctaaatatct ctgttgcaac cggcgttttg
     8521 cttggtgaat ggtggcgtca gaataaagcc tgataaatca gccgggtggc aacgctgcca
     8581 tccggcgttt tcctgcaaat ttactcactt tctgccggta atactggcat ccagtcaatc
     8641 ggtttctcgc cacgttgttc cagccactga tttgccagca caaaatggtt gcagccaaag
     8701 aacccacgat gcgcagaaag cggtgatgga tgcggcgctt tcagtatgtg atggcgttgc
     8761 ttatcaataa tcgccccttt cttttgcgca tgtgagcccc acaacaaaaa caccacgtct
     8821 tcgcggtgct ggttaatcag gctgattact ttatcggtga aggtctccca gccgaggctg
     8881 gcgtgggaat gtgcctgacc tgcgcgaacc gtcaacacag tattgagtag cagaacgccc
     8941 tgacgcgccc agctttcaag ataaccatga ttagggcggg tgaaaccggg aatagtattt
     9001 tccagctctt tatacatatt cagcagtgac ggcggtgtcg caatgccggg gcgaacggaa
     9061 aatgccagac catgcgcctg tcccggtccg tgataaggat cctggccgag aatcaccact
     9121 ttaacgtcac ccaactctgt aaagcggaac gcgttaaaga catctttttg tggcggatag
     9181 atagtgacgc cggactgccg ctcgctggcg acggtctgta gggtattaag aaaatagggt
     9241 tgctgcttct cttcagccag cacatcatgc caggttaatt cgtttgccat ctcgctctcc
     9301 cgcgaatctt caatccgcct agcttaactg cttctttcta cagaacaaaa tcatgctcat
     9361 tttttagact taaacgagtg caaaaaaatg taaattggta aaatttcaca cctgcataag
     9421 ttgatgtaaa acaataaaat ccatttgcaa ccatacttga tgtgtggttt ttattgattt
     9481 aaatcaaaga ttcaagggtg tttgaggagt atatatacac tcaagcaaca atggttttac
     9541 caattggccg cgacaggctg aacaaatcaa ataattttgc cggggaggca tcacatgatt
     9601 acaggtatcc agattactaa agccgctaac gacgatctgc tgaactcttt ctggctgctg
     9661 gacagcgaaa aaggcgaagc gcgttgcatc gttgcgaaag caggttttgc agaagacgaa
     9721 gtggttgcag taagcaaact gggtgacatt gagtaccgtg aagttccagt agaagtgaaa
     9781 ccagaagttc gcgttgaagg tggtcaacat ctgaacgtaa acgttctgcg tcgtgaaact
     9841 ctggaagatg cagttaagca tccggaaaaa tatccgcagc tgaccatccg tgtatccggt
     9901 tatgcagttc gctttaactc tctgactccg gaacagcagc gcgacgttat tgctcgtacc
     9961 tttactgaaa gcctgtaagg cttaagtagc aagttactaa gcggaagagg gcgacttctt
    10021 ccgctttttt tcgttaatag aaaatgcgta ccgcgcaata gaccagcagc agcgcgagca
    10081 cgatatttaa ctggcgacca tactggcgaa acaatcgctg aaacaggtgc cccgccagcg
    10141 cccagcacac attgccaaac gtcccaatca tcgccagcaa aacgctgacg ccaattatcc
    10201 agcttagcgc ctgcgtttgc ggcaggacaa acgtcgacag tgctgtaatg ccatacaaaa
    10261 tgattttgac gttcacaaac tgcaaagcaa agctggccca aaaactgatt ggttttgtct
    10321 gaagcccatc ttcctttgtt ggactggtag cgattttcca cgccagccag acaatatatg
    10381 ccgcccccgc ccaactcaac agatgcactg ctgccgggtc gataacagcc agtgaaaacg
    10441 aaattcccgc gcatagcaac atgacgatta aaaatcccag actcatccct gccagcacgc
    10501 gagtactctg acgaaatccg tgcgttgtgg ctgagctaag ggcgagaata ttgttcggtc
    10561 ctggcgtcat ggcggtaatc agggtgtaag tccaaaaagc acttaaaagg gtcggtgtca
    10621 cttgttatct cctctgtttt ggtagacagt acccgacacg gaacatttaa aaaaatgaat
    10681 gtttttacat tctaaattcg atattttcga taggtttggg gttatggatc tgcgccgttt
    10741 tattacactt aaaaccgtgg tggaagaggg ttcctttttg cgagcttcgc aaaaattgtg
    10801 ctgcacacaa tcgacggtga cttttcatat tcagcagctt gagcaggagt tctcagtcca
    10861 gttatttgag aaaattggtc gacgaatgtg ccttacccgc gaaggaaaaa agctgctgcc
    10921 gcacatttat gaactcacca gagtgatgga tacccttcgt gaggctgcca aaaaagagtc
    10981 ggatccggac ggggaactac gcgttatttc aggcgaaacg ttgctctctt atcgaatgcc
    11041 gcaggtgttg cagcgttttc ggcaacgcgc tccaaaagtg cgtttatcgt tgcagtcgct
    11101 gaactgttac gtgatccgtg atgcgctact gaatgatgag gccgatgtcg gggtttttta
    11161 tcgcgtaggg aatgatgatg ctttgaaccg acgagagttg ggtgaacaac cgctggtgct
    11221 ggtggcttca ccgcaaattg cagatgtcga ttttactgag ccgggaagac ataacgcctg
    11281 tagctttatt atcaacgaac cacaatgtgt cttccggcag atatttgaga gcacgctgcg
    11341 ccagcgacgg atcacggtgg aaaacaccat tgagcttata agtatcgaaa gcatcaagcg
    11401 ttgtgtcgcg gcaaatatcg gggtcagtta cctgccgcgc tttacagtgg caaatgaact
    11461 tgcgagtggc gaattaatcg aattgccttt tggcgaacag tcgcagacca ttacggccat
    11521 gtgcgcacac catgctggaa aagcggttag cccggcgatg cacactttta ttcagtgtgt
    11581 tgaagagtgt tttttgccgg gataaaaaca atgccgggct taaacccggc attgagatta
    11641 ctcttctgtc gtttgcggtg gcacgcccgt tccgcttggt ttacggcgct taccaatatt
    11701 tttagtgtcg cgatggcgtt ttttcacccg cggcttctct ttctctttag cttttttctt
    11761 ctcggcacgt ttagccaaca ctttcttcga tggcttgccg gtctgcttct cgcttggcgc
    11821 acgcgttttc ggacgtaact catcaattac gcgagcttta attggctctt caatataacg
    11881 gccaactttg cccagcagca gatggtcatg ggcttccacc agcgaaatcg cggtgccttt
    11941 acgaccggcg cgcgcggtac gtccgatacg gtgcaaataa gtatcgccac tgcgcggcat
    12001 atcgaagtta aagacgtggc tgacgtcagg aatgtcgata ccgcgcgcgg caacatcggt
    12061 agcaaccagt acgtttacgc ggccttcggt caagcgttta atcgcttcgt tacgcttgcc
    12121 ttgtaccatc tcaccttcga gatagcagtt gttgatgcct gcttcgcgca gccagtttgc
    12181 cagctcatgc acacgctcac gcttacgcac aaacacaatt gagcgggtcg cttccggctg
    12241 ttttaacaga tgcaccagca acgcggtttt atgctcaaga tcatcggcgc ggtaatacca
    12301 ctgatgaatt tttttgcgct cacgggtgga cggattggca gaaacctcca ccggatcttc
    12361 cagcagacgc tcggcaaagt cctgaatcgc atcgccttcc agagttgccg aaaagagcag
    12421 ggtctgttta cgccagcgcg tttcgccagc aatatgttcg atatcctggg cgaagcccat
    12481 atccagcata cggtctgctt cgtcgaggat cagcgtttca accgcgcggc aatcgaagtt
    12541 ctcttctttt atgtattgca gcagacgtcc ggtcgtggcg accacgatgt cctgattttc
    12601 gctgaacact tccgcgtggt tcatataggc tacgccgccg gtgatggtgg caatatccag
    12661 atgcgtatgt ttcgccagtt cgcgggcatg atcggccacc tgcatcgcca gctcgcgagt
    12721 tggggtgagg atcaaaatac gcggcggacc ggatttctta cgtgggaaat cgagcaggtg
    12781 ctgcaacgct ggcagcagat acgccgccgt tttaccggtg cctgtcggcg cagaaccgag
    12841 tacatcacgg ccatcgagcg caggcggaat ggcggcagcc tgaatggcgg tcgggcgagt
    12901 gaaaccttta tcctggaggg cttccagcag gctttcgtcg agttcaagtt cggaaaaagt
    12961 cgttacagtc atgttctacc tctgtgtggg gcgctgatta tagacgttac ggctgcaatc
    13021 ttcatctgtt tgtatggata tcgcttctcg ggtattgttc tcacccggtg ctatcgccgt
    13081 ttttcctgca aggttttatt ttcatgtcac agtctacatc cgtgcttcgt cgtaatggat
    13141 ttacttttaa acagtttttt gttgctcacg atcgttgtgc gatgaaagtg ggaacggatg
    13201 gcattttgct gggcgcatgg gcaccggtgg ctggggtaaa acgttgcctt gatatcggcg
    13261 cgggtagcgg gttgctggca ttaatgctgg cgcagcgaac cgatgacagc gtaatgattg
    13321 atgcagttga actggaaagt gaagctgccg cgcaggcgca ggaaaatatc aaccagtccc
    13381 cgtgggcaga gcggattaac gtccatacgg cggatattca gcagtggatc acacagcaga
    13441 cagtacgctt cgatttaatc atcagtaacc caccttacta tcagcaggga gtagagtgcg
    13501 cgacacctca acgggaacag gctcgctata ccactacgct tgatcaccca tcgttgctga
    13561 cctgtgcggc ggagtgcatt accgaagagg gatttttctg cgtggtgctg ccagagcaga
    13621 ttggtaatgg ttttactgaa ctggcattaa gtatgggctg gcatttacgt ttgcggacgg
    13681 atgtggcgga aaacgaagcg cgactgccgc atcgggtgct gctggcattc tccccgcagg
    13741 cgggagaatg ctttagcgat cgcttagtca ttcgtgggcc agaccagaac tattccgaag
    13801 cgtatacggc gctgacccag gctttttatc tgtttatgta atgattgccg ggggaaagga
    13861 tcgacggacc ggaatgggtg agcagttccg gataatccag cgtgaaatgc aacccccgac
    13921 tctctttacg catcattgca cagcgaacaa tcaactcggc aacctgtacc agattacgca
    13981 gctccagcaa attatttgag acgcggaaat gggcgtaata ttcgtctatt tcttgctgga
    14041 gcatggttat ccgccgcagg gcgcgttcca ggcgcttcgt tgtgcgcaca atgccgacgt
    14101 aatcccacat aaacagacgt agctcgtgcc agttatgttg aatcaccacc cgttcatcag
    14161 ggttctcaac gcggctttca tcccacggcg gtaacgtact gacgccgtgg gcataaggca
    14221 tacatctggt gatatcttct gccgccgacc agccatagac cagacactcc agcaaggagt
    14281 tagaggccat gcggttagcg ccgtgtaagc cggtataact cacttcgcca atggcataca
    14341 aaccctcgac gtccgtacgc ccatgatcat ccaccatcac accaccgcag gtatagtgtg
    14401 cagcaggcac aatcggtact ggttcttgtg tgagatcaat ccccagcccg agcaattttt
    14461 cataaatcat cgggaaatgc tggcgaataa aatcggcggg cttatggctg atgtcgagga
    14521 acatacaatc cgcgccgagg cgtttcattt catggtcaat agcgcgggcg acaatatcgc
    14581 gcggggccag ttcgccgcga tcatcaaaat cgggcataaa acgcgtacca tccgggcgct
    14641 tgagataagc gccttcgccg cgcagtgctt ctgttaacag gaaattgcgt gcctgtgggt
    14701 gatacagtgc ggtagggtgg aactgattaa attcgagatt ggcaacccgg cagcctgcgc
    14761 gccacgccat agcaatgcca tcgccagaag aaatatccgg attggtggtg tactgataaa
    14821 ccttcgacgc accgccggtt gccagtacca ctgcttttgc gtggcaggtt tccaccgttt
    14881 ctttatttcg gttccatacc cacgcgccaa caacccgtcg cgtgcccggc aggccaattt
    14941 tgtcagaaac aatcagatca accgcgttgc tgcgctccag cacgcgaata ttcggatggt
    15001 tcagcgcctt gctcaccagc gtggtttcta cttctctacc ggtggcgtcg gcggcatgaa
    15061 gaattcgacg gtgactatgt ccaccttcac gggtcagatg gtagctttct gcaccattcg
    15121 gttgaatgtg ggtatcaaac aacaccccct ggtcgattag ccattgcaca caggatcgtg
    15181 cattgctggc gacaaattca actgcatgac gatcgcaaat accagccccg gcaatcaatg
    15241 tgtcttccac atgcgagtca atgctgtcag tctcatcaaa cacggcggct ataccgccct
    15301 gggcataaaa tgttgaacct tccgttaccg ggcctttact tagaacgatg acctgatgct
    15361 ggtcagccag gcgtagcgcc agtgaaagtc cagctgcgcc gctaccgata atcaacacgt
    15421 cacatgaatg ttcagggaga gtattcattt tctttgttta atttactaaa catggtttgg
    15481 tcagcatagc atcatgttgt gcgattaaac acctgctatt ttaatatttg ttacagtgac
    15541 taaacacgct gactcagggc ggcgagaaaa agagaagtta ctggctggtg gaggattagg
    15601 tggtgaaata aaaaggccgt tgggttactc ttcaggcagt taaatgggca tttctacaca
    15661 gataatgcga tgttcagatt ctgtagactt ataatgatag ataatgatcc gtctacagca
    15721 tgacaaacaa aaacagatgc gttacggaac tttacaaaaa cgagacactc taaccctttg
    15781 cttgctcaaa ttgcagctaa tggagtggcg tttcgatagc gcgtggaaat ttggtttggg
    15841 gagactttac ctcggatgag cgagcagtta acggaccagg tcctggttga acgggtccag
    15901 aagggagatc agaaagcctt taacttactg gtagtgcgct atcagcataa agtggcgagt
    15961 ctggtttccc gctatgtgcc gtcgggtgat gttcccgatg tggtacaaga agcttttatt
    16021 aaagcctatc gtgcgctgga ttcgttccgg ggagatagcg ctttttatac atggctgtat
    16081 cggattgctg taaatacagc gaaaaattac ctggttgctc aggggcgtcg tccaccttcc
    16141 agtgatgtgg atgccattga agctgaaaac ttcgaaagtg gcggcgcgtt gaaagaaatt
    16201 tcgaaccctg agaacttaat gttgtcagaa gaactgagac agatagtttt ccgaactatt
    16261 gagtccctcc cggaagattt acgcatggca ataaccttgc gggagctgga tggcctgagc
    16321 tatgaagaga tagccgctat catggattgt ccggtaggta cggtgcgttc acgtatcttc
    16381 cgagcgaggg aagctattga taacaaagtt caaccgctta tcaggcgttg acgatagcgg
    16441 gatactggat aagggtatta ggcatgcaga aagaacaact ttccgcttta atggatggcg
    16501 aaacgctgga tagtgagctg cttaacgaac tggctcataa cccagaaatg cagaaaacct
    16561 gggaaagcta tcacttaatc cgtgactcaa tgcggggtga tactcccgag gtgctccatt
    16621 tcgatatctc ttcacgcgtg atggccgcca ttgaagaaga gccagtacgt caaccggcga
    16681 cattgatccc ggaagcccag cctgcgccgc atcaatggca gaaaatgcca ttctggcaga
    16741 aagtacgtcc gtgggcggca cagcttaccc aaatgggcgt agccgcatgc gtatcgcttg
    16801 cagttatcgt tggcgtccag cactataatg gacaatctga aacgtcccag cagcccgaaa
    16861 cgccggtatt taatacactg ccgatgatgg gtaaagccag cccggtaagc ctgggagtac
    16921 cttctgaagc gaccgcaaac aatggtcaac agcagcaggt acaggagcag cgtcgtcgca
    16981 ttaatgcaat gttgcaggat tacgaactgc aacgccgact ccactctgaa cagcttcagt
    17041 ttgagcaggc gcaaactcag caagccgctg tacaggtgcc aggaattcaa actttaggaa
    17101 cgcaatcgca gtaatgaagc aactttggtt tgccatgtca ttagtgacag gtagcctgtt
    17161 attctctgct aacgcctcgg ccactcccgc gtccggggcg ttattacagc agatgaacct
    17221 ggccagtcag tcactgaatt acgagctgtc attcatcagc atcaataaac agggtgttga
    17281 gtctctgcgt tatcgacatg cacgcctcga taaccgtcct cttgcacaat tgttgcaaat
    17341 ggatggcccg cgccgggaag tggtacagcg cggcaatgaa atcagctatt ttgaaccggg
    17401 acttgaaccg ttcacgctta atggcgatta cattgttgat tctctaccat cgctcatcta
    17461 taccgatttc aaacgccttt ctccttacta cgactttatc tccgtcgggc gcacgcgtat
    17521 tgctgatcgt ctttgcgaag tcattcgtgt ggttgcccga gatggtacac gctacagcta
    17581 catcgtgtgg atggacaccg aatcgaaatt accgatgcgg gttgatctcc ttgatcgcga
    17641 tggtgaaacg ctggaacaat ttcgcgtgat tgcttttaat gtcaatcagg atatcagcag
    17701 cagtatgcag acgctggcga aggcaaattt gccgccgttg ctttctgttc ctgtaggtga
    17761 aaaagctaaa ttcagctgga cgccaacctg gttgccacag ggttttagcg aagtttccag
    17821 tagtcgacgt ccgctaccga cgatggacaa catgcctatc gaatcacgtc tctattccga
    17881 cggattattc agcttctcgg taaacgttaa ccgcgctacg ccatcgagca ccgatcagat
    17941 gttgcgcacc gggcgcagaa ccgtcagtac aagcgtacgt gataacgccg aaatcaccat
    18001 tgtcggtgaa ctgccgccgc aaacggcgaa acgcattgcc gagaatatta agttcggggc
    18061 agcgcaatga tcaaagagtg ggctaccgtc gtctcctggg aaaacgggca ggcgctggtt
    18121 agttgtgatg ttaaagcctc atgcagcagc tgcgcttcac gtgccggttg cggtagccgc
    18181 gtgttgaata aacttggccc gcaaaccacg cataccattg tcgtaccctg tgatgagccg
    18241 ttagtgccgg ggcaaaaagt agaattagga atcgccgaag gcagcctgct tagctccgca
    18301 ttactggttt atatgtcgcc gttggtggga ctattcctca tcgcttcact atttcagcta
    18361 ctctttgctt cagatattgc agcattatgc ggtgcgattc tcggtggcgt tggtggattc
    18421 ctgattgcac gtggctgctc tcgaaagttt gctgcccggt ctgaatggca accgatcatc
    18481 ttaagcgtgg ccttgccgcc tggccttgtg cgatttgaaa catcttcgga agacgcgagc
    18541 cagtgatttc actggctttg ttgcattttg cataacaaat gcgaggacgt ttccagatac
    18601 cacaatccat acggtatgaa catttcctcg cctcaatgtt gtagtgtaga atgcggcgtt
    18661 tctattaata cagacgttaa gctcagaaca gcgacttcta aaagcctggt taaccgggca
    18721 ttaaggcaca ataatcatac tttatgaaga atatacgtaa cttttcgatc atagctcaca
    18781 ttgaccacgg taaatcgacg ctgtctgacc gtattatcca gatctgcggt ggcctgtctg
    18841 accgtgaaat ggaggcgcag gttctcgatt ccatggatct tgagcgtgag cgtggcatta
    18901 ccatcaaagc gcaaagcgtg acgctggact acaaagcgtc tgacggcgaa acctatcagc
    18961 ttaactttat cgacaccccg ggccacgtag acttctccta tgaagtttct cgttcgctgg
    19021 ctgcctgtga aggtgcattg ctggtggtcg acgccgggca gggcgtagaa gcgcaaaccc
    19081 tggcaaactg ctacaccgcc atggaaatgg atctcgaagt ggtgccggta ctgaacaaga
    19141 ttgacctgcc ggcagccgat cctgaacgcg tggcggaaga aattgaagat atcgtcggca
    19201 tcgacgccac cgacgcggtg cgctgttcag cgaaaaccgg cgttggcgta caggacgttc
    19261 tcgaacgtct ggtgcgcgac attccgccgc cggaaggcga tccggaaggc ccgttgcagg
    19321 cactcattat cgactcctgg ttcgacaact acctgggcgt tgtttcactt atccgtatta
    19381 aaaacggcac cctgcgtaag ggcgacaaag tgaaagtcat gagtaccggg cagacctata
    19441 acgccgaccg tctgggcatc ttcacgccga agcaggttga ccgtactgaa ctgaaatgcg
    19501 gcgaagtagg ctggctggta tgtgcgatta aagatatcca cggcgctcca gtaggcgata
    19561 ccttaacgct ggcgcgtaat ccggcagaaa aggcgctgcc tggctttaag aaagtcaaac
    19621 cgcaggtata cgccggtctg ttcccggtaa gttccgacga ctatgaagcc ttccgtgacg
    19681 cgctgggcaa actcagcctg aacgatgctt cactgttcta tgagccggaa agctccagcg
    19741 cgctgggctt tggtttccgc tgcggcttcc tcggcctgct gcacatggag atcatccagg
    19801 aacgtctgga acgtgaatac gatctggatc tgatcaccac tgcgccgacc gtagtgtatg
    19861 aagttgaaac cacgtcaaga gaagttatct acgtcgacag cccatccaag ctgccagcgg
    19921 taaataacat ttacgaactg cgcgagccga ttgcagagtg tcacatgctg ctgccgcagg
    19981 catatctcgg caacgttatt acgctgtgcg tagaaaaacg cggcgtgcag accaatatgg
    20041 tttaccacgg taatcaggtg gcgctgacgt acgagatccc gatggcggaa gtggtgctcg
    20101 acttcttcga tcgcctgaaa tctacctcgc gtggttatgc gtctctggat tacaacttca
    20161 aacgcttcca ggcgtccgac atggtacgtg tagacgtatt aatcaacggt gaacgtgttg
    20221 atgcgctggc gttgatcacc caccgtgata attcgcaaaa ccgcggtcgc gagctggtgg
    20281 agaagatgaa agatctgatc ccacgccagc agtttgatat cgccattcag gcagcgattg
    20341 gtacgcacat cattgcgcgc tccaccgtga aacagctgcg taaaaacgta ctggctaaat
    20401 gttatggcgg cgatatcagc cgtaagaaaa agctgctgca gaagcagaaa gaaggtaaga
    20461 aacgcatgaa gcagatcggt aacgtcgagc tgccgcagga agcgttcctc gccattctgc
    20521 acgtcggcaa agacaacaaa taacccttag gagttggcat ggcgaatatg tttgccctga
    20581 ttctggtgat tgccacactg gtgacgggca ttttatggtg cgtggataaa ttctttttcg
    20641 cacctaaacg gcgggaacgt caggcagcgg cgcaggcggc tgccggtgac tcactggata
    20701 aagcaacgct gaagaaagtt gcgccgaagc ctggctggct ggaaaccggt gcttctgttt
    20761 ttccggtgct ggctatcgta ttgattgtgc gttcgtttat ttatgaaccg ttccagatcc
    20821 cgtcaggttc gatgatgccg actctgttaa tcggtgattt tattctggta gagaagtttg
    20881 cttatggcat taaagatcct atctaccaga aaacgctgat cgaaaccggt catccgaaac
    20941 gcggcgatat cgtggtcttt aaatatccgg aagatccaaa gcttgattac atcaagcgcg
    21001 cggtgggttt gccgggcgat aaagtcactt atgatccggt ctcaaaagag ctgacgattc
    21061 aaccgggatg cagttccggc caggcgtgcg aaaacgcgct accggtcact tactccaacg
    21121 tggaaccgag cgatttcgtt cagaccttct cacgccgtaa tggtggggaa gcgaccagcg
    21181 gattctttga agtgccgaaa aacgaaacca aagaaaatgg aattcgtctt tccgagcgta
    21241 aagagacatt gggtgatgtg acgcaccgca ttctgacagt gccgattgcg caggaccagg
    21301 tggggatgta ttaccagcag ccagggcaac aactggcaac ctggattgtt ccgccgggac
    21361 aatacttcat gatgggcgac aaccgcgaca acagcgcgga cagccgttac tggggctttg
    21421 tgcctgaagc gaatctggtc ggtcgggcaa cggctatctg gatgagcttc gataagcaag
    21481 aaggcgaatg gccgactggt gtgcgcttaa gtcgcattgg cggtatccat taatagccat
    21541 cttcgttcac gttgtcgccg ttatggcgac aacgtgaatt atttatgaga taaatctccc
    21601 gtggctaacg acatcccccg tcgttgtgta tagaatattc ccccgaagtt taaggttggc
    21661 acctccaggt tgccacggca cacgaaacag cgttggtccc catataccgg taaactgaaa
    21721 ctgcagtgaa gcagttagcc gagccatgta tatcaggtct gtttcgtgtg ctgaattgtt
    21781 gacgcattta tttattggta tcgcatgaac cccatcgtaa ttaatcggct tcaacggaag
    21841 ctgggctaca cttttaatca tcaggaactg ttgcagcagg cattaactca tcgtagtgcc
    21901 agcagtaaac ataacgagcg tttagaattt ttaggtgact ctattctgag ctacgttatc
    21961 gccaatgcgc tttatcaccg tttccctcgt gtggatgaag gcgatatgag ccggatgcgc
    22021 gccacgctgg tccgtggtaa tacgctggct gaactggcgc gcgaatttga gttaggtgag
    22081 tgcttacgtt tagggccagg tgaacttaaa agcggtggat ttcgtcgtga gtcaattctc
    22141 gctgacaccg tcgaggcatt aattggtggc gtattcctcg acagtgatat tcaaaccgtc
    22201 gagaaattaa tcctcaactg gtatcaaact cgtctggacg aaattagccc aggcgataaa
    22261 caaaaagatc cgaaaacgcg tctgcaggaa tatttgcagg gtcgccatct gccgctgcca
    22321 acttatctgg tagtccaggt acgtggcgaa gcgcacgatc aggaatttac tatccactgc
    22381 caggtcagcg gcctgagtga accggtggtt ggcacaggtt caagccgtcg taaggctgag
    22441 caggctgccg ccgaacaggc gttgaaaaaa ctggagctgg aatgagcatc gataaaagtt
    22501 actgcggatt tattgctatc gtcggacgtc cgaacgttgg caaatccaca ttgttgaaca
    22561 aactgctggg gcagaagatc tccatcacct cccgcaaggc gcaaacaact cgtcaccgca
    22621 ttgtggggat ccatactgaa ggcgcgtatc aggcgatcta cgttgatacc ccaggcctgc
    22681 atatggaaga aaaacgcgcc attaaccgcc tgatgaacaa agcggcgagc agctctatcg
    22741 gcgacgttga gctggtgatt tttgtcgtag aaggcacccg ctggacgccg gacgatgaaa
    22801 tggtgctcaa caaactgcgc gatggcaaag cgccggtaat cctcgcggtg aacaaagtgg
    22861 ataacgtgca ggaaaaagcc gatctgctgc cgcacttaca gttcctggca agccagatga
    22921 acttcctgga tattgtgccg atctctgccg aaaccgggct gaatgtcgac actatcgcag
    22981 caatcgtgcg taagcatctg cctgaagcga cccatcactt cccggaagat tacatcaccg
    23041 atcgctcaca gcgttttatg gcgtctgaaa tcatccgcga aaaactgatg cgtttcctcg
    23101 gcgctgaact gccgtactcc gtgaccgtgg agatcgaacg tttcgtctct aacgaacgcg
    23161 gcggttatga catcaacggt ttgattctcg ttgagcgtga agggcagaag aagatggtta
    23221 ttggcaacaa aggggccaag atcaaaacca tcgggattga agcgcgtaaa gacatgcagg
    23281 aaatgtttga agcgcctgtt caccttgagc tgtgggtaaa agtgaaatcc ggttgggccg
    23341 acgacgaacg cgcactgcgc agtctcggtt atgttgacga tctttaagag taactccgat
    23401 ggaaggctgg cagcgcgcat ttgtcctgca tagtcgcccg tggagcgaaa ccagcctgat
    23461 gctggacgtc ttcacggagg aatcgggtcg cgtgcgtctg gttgccaaag gcgcacgctc
    23521 taaacgctct actctgaaag gtgcattaca acctttcacc cctctcttgc ttcgcttcgg
    23581 tgggcgtggt gaagtcaaaa cactgcgcag tgctgaagct gtctcgctgg cgctgccatt
    23641 aagcggtatc acgctttaca gcggtctgta catcaacgaa cttctctccc gcgtactgga
    23701 atacgagacg cgcttctctg aactcttttt cgattacttg cactgcattc agtcacttgc
    23761 aggtgccact ggtacgccag aacccgcgct gcgtcgcttt gaactggcac tgctcgggca
    23821 tctgggctat ggcgtcaatt ttacccattg tgcgggtagc ggcgagtcgg tagatgacac
    23881 catgacgtat cgttatcgcg aagaaaaagg gtttatcgca agcgtcgtta tcgacaataa
    23941 aacgttcacc ggaaggcagt taaaagcgtt aaacgcgcgg gaatttcctg acgcagacac
    24001 actgcgcgcc gcgaaacgct ttacccgcat ggcgcttaag ccgtatcttg gcggtaaacc
    24061 attaaagagc agggaactgt tccggcagtt tatgcctaag cgaacggtga aaacacatta
    24121 tgaatgatga ggattgtcat ggctgaatta ctgttaggcg tcaacattga ccatattgca
    24181 acgctgcgta acgcacgcgg tactgcttac ccggatccgg tgcaggcggc gtttattgct
    24241 gagcaggcgg gagcggacgg cattaccgtg catttacgtg aagatcgccg ccacattact
    24301 gaccgcgatg tgcgcatcct gcgtcagacg ctggataccc gcatgaatct ggagatggcg
    24361 gtgaccgaag agatgctggc gatcgccgtt gagacgaagc cacatttttg ctgtctggta
    24421 ccggaaaagc gtcaggaagt aacaaccgaa ggcggcctgg acgtcgcagg gcagcgtgac
    24481 aaaatgcgcg atgcctgtaa acgtctggcg gaggccggga tcccggtttc tctgtttatc
    24541 gacgccgatg aagagcagat caaagctgcg gcagaggttg gcgcaccgtt tatcgagatc
    24601 cacaccggtt gctatgctga tgccaaaact gacgccgaac aggcgcaaga gctggcgcgt
    24661 atcgccaaag ccgcgacctt tgccgctagt ctcggtctga aagttaacgc tggtcacggt
    24721 ctgacctatc acaacgtgaa agctattgcc gccatccctg agatgcatga actgaatatc
    24781 ggtcatgcca ttattggtcg tgcagtgatg accggactga aagatgcggt ggcagaaatg
    24841 aagcgtctga tgctggaagc gcgtggctaa tggcaatatt aggtttaggt acggatattg
    24901 tggagatcgc tcgcatagaa gcggtgatcg cccgatccgg tgagcgcctg gcacgccgcg
    24961 tattaagcga taacgaatgg gcaatctgga aaacgcacca ccagccggtg cgttttctgg
    25021 cgaagcgttt tgctgtgaaa gaagcggcgg caaaagcgtt tggcaccggg atccgcaatg
    25081 gtctggcgtt taatcaattt gaagtattca atgatgagct cggcaaacca cggctacggc
    25141 tatggggtga ggcattaaaa ctggcggaaa agctcggcgt tgtaaatatg catgtaacgc
    25201 tggcagatga acgccactat gcttgtgcca cggtaattat tgaaagttaa aaaaataccg
    25261 ttctctttat aaagtaaaac ccccgaactg gtaaggaacg ggggttttca ctttacaact
    25321 ttaattttgt gcagcacacg attccaacag aaatgattat ggatcaattt cgttatcctg
    25381 tacaagcaac ttgcaatctc tgacataaaa aacgcgagcc tttacgaaga aatcgacatt
    25441 gataaaaaga ttaacgtgac cgctttttga taagtacatt aaaatcggtt tcgttattgg
    25501 caattttgct gtgctttaca ccatgccact gaattccccc attgaaacga gtggtgtcgt
    25561 caaagctctg gtgtggagtg cagcactcga ttccaataac tcgcacgttc agttttgggg
    25621 tgatgtaagg gctaatctga acggctgcat tccttgttta aggaaaaacg aatgactgat
    25681 tgccgatacc tgattaaacg gattatcaaa atcatcattg ctgttttaca gctgatcctt
    25741 ctgttcttat aacacaagga aacgtactta aggtgcgtcc ggtgaaccag t
//
LOCUS       DAZGAP010000068        25618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGAP010000068 DAZGAP010000000
VERSION     DAZGAP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.068"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..493
                     /locus_tag="ACHLAM_002301"
     CDS             <1..493
                     /locus_tag="ACHLAM_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853819.1"
                     /translation="WRTQTYKIIKLNNSIEHPANLQIEDIQNLSTGRPVLLGDNLHEL
                     NEFIENTRQQFTPNTINWWRTPFIALNNWINQETVNTIGGYIQMVTAHPLGTRISFLT
                     NLNENIIRTSYAGINTTESFGPTIGGFILMPMDGMSLPDENGWDVGNQVTRTAADRVA
                     TNR"
     gene            complement(496..720)
                     /locus_tag="ACHLAM_002302"
     CDS             complement(496..720)
                     /locus_tag="ACHLAM_002302"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7853820.1"
                     /translation="MKNTSSIQAQFGAHLKKLRLQSGLSQEAFADKCGLDRTYVSGIE
                     RGVRNPTLEVISVLGKGLDMDIKELFNFER"
     gene            complement(760..1023)
                     /locus_tag="ACHLAM_002303"
     CDS             complement(760..1023)
                     /locus_tag="ACHLAM_002303"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7853821.1"
                     /translation="MEKTGSIKKVLGKRIRALRREMDLSQEALADKCGIDRTYMSGIE
                     RGVRNPTLVVICAISDGLGMDLSHLFMFDDGAEKDAESTKCDS"
     gene            1131..1406
                     /locus_tag="ACHLAM_002304"
     CDS             1131..1406
                     /locus_tag="ACHLAM_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7853822.1"
                     /translation="MTKKDWHPADIIAALHKRGTSLAKLSREAGLASSTLANALNRPW
                     PKGEWLIAIALGKHPSEIWPERYLNTGNKREPRKPLHLMTTTENSGT"
     gene            1625..1999
                     /locus_tag="ACHLAM_002305"
     CDS             1625..1999
                     /locus_tag="ACHLAM_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO7853823.1"
                     /translation="MMTYCARIVAIGLFVADGLADRTLITFDSNGPKDCLDYSLSLEP
                     SFREDDLLILPGDRLLLASHDYLVTAVGKEAQQSLFELGHLTMVFNGSLNACHSGAVH
                     LSGPIPKLHDLYGELLIEEGRP"
     gene            2043..3401
                     /locus_tag="ACHLAM_002306"
     CDS             2043..3401
                     /locus_tag="ACHLAM_002306"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7853824.1"
                     /translation="MELNRSLKARHLTMISVGGVIGAGFFLGAGAAIARTGPSVVLAY
                     LIGGVITLFIMLLLAEMAVARPVSGSFESYARTAFGPWCGFITGWTYWLAFLIGPASE
                     AIAAGTFLNLWFPGVPVWLFCLVIASSLTAINMVGVHFFGEVEFWLSLVKVVALLAFI
                     VAGCYSLGLDNASPASISHPTDSGSFFAGGLPGFLGAMLMVIFSFGGTEAIGTAAGES
                     ENPQRDIPRTLRGTVVRILLLYVGSLTVLLLVLPWQQAGVSSSPYVEASGILGGHMAR
                     HIMNFVVLTAALSCIDTGVYATSRMLHAMASDGYFPAWFARLHPAHKTPDNAILASSL
                     VLFTGVIIAILSPDAYVILAGISGFGFLFTWLMIALSQPTLHRIALHDGTLKYKAPAA
                     RYIQPLTVGLIALIMAGQFFTDGGGITLVTVLIWLALASAYYIFVVRKNRRLSDEKSH
                     AQ"
     gene            3379..4170
                     /locus_tag="ACHLAM_002307"
     CDS             3379..4170
                     /locus_tag="ACHLAM_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HFO7853825.1"
                     /translation="MKKVMLNELTGREIRELLQSGKELTAIVNFGSCENHADHLPLGP
                     DMFVPEIVACRVAQRVDNTLVLPCTPFGTSVHYNAYPLAVTLRYETNIALAEDIFISL
                     LHHGIRRILVINGHDGNIPAIEIAARNVKARHTQAAIVVVPAWWDLVGHHLGKGFFDS
                     WDNTGLGHGGEGETSVMMALIPELVELKYAQKQIPVEVFADYGVNRIWDIGEISATGA
                     TGDPRCATAEKGQRILACLVDVLCALLRKLDAANWQYALNKDANM"
     gene            complement(4273..4692)
                     /locus_tag="ACHLAM_002308"
     CDS             complement(4273..4692)
                     /locus_tag="ACHLAM_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853826.1"
                     /translation="MANQQAAKWAGVAAIAACLTFVVTTIGLLLAWRSLHQWKPQYKE
                     NSRLLLIEALIAFQKCLITIPKNLDNDPTYQSRKEFLKASTEVELRGQIYLKQHSNEK
                     LKDELANLRSKCAEFVGGKVTKPELSFISAIILLIEV"
     gene            complement(4931..5821)
                     /locus_tag="ACHLAM_002309"
     CDS             complement(4931..5821)
                     /locus_tag="ACHLAM_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HFO7853827.1"
                     /translation="MISQCFDSRFYTFKVESLVYARTLPVNVKFSTKYKQIKASVDDI
                     GLIEPIVVYINDANDKVILDGHLRVEVLKELGITTAHCLISPVEDTYSYNKRVSRLTI
                     VQAQNMLLKAVESGVSVEKLCAVLGVTPDTLIGKLKISDGIAPEVMALLAEKNVPQTI
                     FSVLKKLKFYKQIEMVNTMISINNFTRKFAMSMLHAVAPEHLVAPKKDASRETDIRKN
                     LERLEKEMASVQVETQHLQDEYAENNLRLVIIKNHIERLLANGQVLNWLYDNHQEYLS
                     VLKQISGLDDLNKLGQTIEE"
     gene            complement(5823..6710)
                     /locus_tag="ACHLAM_002310"
     CDS             complement(5823..6710)
                     /locus_tag="ACHLAM_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7853828.1"
                     /translation="MKSFDEQIELVDISDIYIANPRKRNKFIHNEIKENIKTIGLKRP
                     VSVRPVNNENYKYALICGQGRLEAYIEYGETKIPAIIKQVDEETAHLMSLAENIARRN
                     PRAAELMEDIRRMKTSGLTDREIGLRLGYNSSWVNGVITLLSSGEKHLLRAFETGDIP
                     LYLAVEISRSGDEETQELLTVALKEGHIKGGQINKIRRILEQRKKGDKGTISTAFFPG
                     KKQKKLTPEELANVYQENINEQKAILLKSTMVKEKILAVKQIFGYLLKDNDFVTLLHS
                     NNIHDIPSLLDGSDVSGEK"
     gene            complement(6710..8245)
                     /locus_tag="ACHLAM_002311"
     CDS             complement(6710..8245)
                     /locus_tag="ACHLAM_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7853829.1"
                     /translation="MSDIVAGNRIAAQYLRMSTEHQKYSPENQREYIEKYAKEHCITI
                     KYTYDDAGRSGLSLDNRPGLRSLLYDVENGIIKVSLILVYDVSRFGRFQDIEEAAHYS
                     WLLKRNGVRIIYCAEPFSEEQTELFMLGLSYSRYGAATYSKNLSQKVFLGQANLVRRG
                     YYQGGMPGYGIRRLLIDEHTNPKGMLLPGQRKSLQTDRVILIPGPAEEVVIVNKIYDM
                     FIDEGKPEFVIASELNRKNIPAENGLQWSRCKIHQILTNEKYIGNNVYNRTSGRLKTR
                     SVRNPQDQWIRCDGAWDPVVSVEKFEAAKRIIEARAVQYSDDELLDKLRHLLKLRGRL
                     SGIVIDEEELFPSSSIFRSRFGGLVRAYTLIGYHPRHDYSYLEIKKQIAECHLTFMDN
                     LVQDIYRYGGWAEMHQDGSLYINDEFFVSANLVRCQRLSSGKLRWKIKFNVSHYCDVM
                     IFPRLDSTNNSVVDYFMFPAIDVLYNDLTLHENNGFILELYRFDDLSCLENLVKRTGL
                     ESK"
     gene            complement(8269..9216)
                     /locus_tag="ACHLAM_002312"
     CDS             complement(8269..9216)
                     /locus_tag="ACHLAM_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7853830.1"
                     /translation="MRKILFFLLLMIAVLPEVQATCSFYGGVTSEVSGYLNFGNVVIQ
                     RDAQVGSTIATAITGAYNNGDGIAGCKREAWTARWELTQWGTLSGYGNGVYNTNLPGV
                     GLRLSAASTGKTLPYETSYAYNAGGTWVSIPGDGIKGELIKTGDITSGTLTDGVLARA
                     SVVNQFYFANVTLNGMNTVKSEACTVTTPSVDVPMGDHDKSEFSGAGSSTDWVSFDIG
                     LSCDVGARINVRIDATADASASSQGVMLIDSGGASGVGIQLHYRPDDAAVQYGQERFY
                     WQSVYGDEIVQLKARYYQTAGSITPGAANGTATFTMSYK"
     gene            complement(9235..10239)
                     /locus_tag="ACHLAM_002313"
     CDS             complement(9235..10239)
                     /locus_tag="ACHLAM_002313"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7853831.1"
                     /translation="MRVWRGMILLAGILFCQASWAFCTSSVSFSISPGTVVVQRDAVV
                     GEPISDWLVSNQGMAYQDCNYDATPQYVINAGIKNTGSRASVTWNGEAVFNSNVAGVG
                     FVFEGRSSVDSNPLPENWIGIASGQSQVFTLQISHSVGYHPSINDQARIRLIKTGDIT
                     PGTLSGNLGRFIAGTRENGQWSTEIPITFSGGQITTIACSVTTPDVNVPLGQHQKAEF
                     SGPGTGTGWQAFNIGLDCDKSARINVQIDAVQDPSNVAGVMKLDSAPGDMAATGIGVQ
                     LFFAPDNSAVQFGQSRYYYTSPNGGTETVQLKARYYQTAATITAGVANATATFTLTYK
                     "
     gene            complement(10236..12752)
                     /locus_tag="ACHLAM_002314"
     CDS             complement(10236..12752)
                     /locus_tag="ACHLAM_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7853832.1"
                     /translation="MNNPTPRLMIILLTLLLAIVLLSGYLRNAEAREVFNPHALEIDN
                     PGQPVADLSTFAESGGQLPGTYRVTVFVNGEKQSDAQDIAFIDGAKKKLSPQITPAML
                     KAWGVKTDVFPALAALPPDQPLDDIGRYIPMANTELRFSKLQLNVSIPQAAMNASARG
                     YVDPSEWDEGVPAALLNYNLSGSNTWRDGQNGSDDNYYANLQSGINLGAWRLRNYSTW
                     NYDEDNGSHWDSVNTYLQRDIQRLKGQLTLGDSYTPSDIFDSVQFRGAQLASDDNMLP
                     DSLRGFAPVIRGIAQSNAQVTIKQNGYIIYQSYVAPGAFTITDLYPTSGSGDLEVTIK
                     EADGSERTFVQPFSAVPIMQREGRLKYALTAGKYRSGNEDSDEPQFGQVTAIYGLPHA
                     ITVYGGTLYSDDYQSGAAGLGFGLGELGSLSADITAAHTTLNNDETHNGQSYRVQYSK
                     DFQTTDTSFTLAAYRYSTAGFYTFQEANDLRADDDNGWQMTYNKRQRLQLDLSQSIGS
                     YGSFFISGYQQDYWQEDGYERTLSAGWNGNIDGISYSVTWSYSDYPESTQPADQQLAF
                     NIQVPLSRFMPNAWASYSVNTAKHGDTRQQVGLNGTALADNNLSYSLQQSYTNHGVGG
                     SGNINADYKGGQGEITGGYNYDDDTQQVNYGLKGGIVAHPHGITLSQPLGQSLAIVKA
                     PGADDTKVQNNTGVYTDWRGYAVVPYVNPYKKNRIALDTSTLGDEVDTDTAVQTVTPT
                     QGAVVMADFNTRVGRRVLMTLLYRGLPVPFGAEAKLKEGGSGIVGDDGQVYLTGVPEE
                     GDIAVNWNGAQQCVVHYRLPEQENQPSVILVNQECREVAK"
     gene            complement(12764..13444)
                     /locus_tag="ACHLAM_002315"
     CDS             complement(12764..13444)
                     /locus_tag="ACHLAM_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7853833.1"
                     /translation="MNRYADVVLALLLAFLSVQAQAGIVIGGTRVIYDGDKKETAASV
                     RNPEKSGVYLVQSWVDSGEQGGKTPFIVTPPLFRINPGEENMLRIVRTGGNLPQDRES
                     VFWLNVKSIPATDDSHSRTNVLQVVVKSRLKLFYRPAGLEGQPESAYHQLSVAHSGNH
                     LTVSNPTPYYVTLFTLKVDGQEIKEADMVPPKGSVSFTLPSATASAVTWQAISDYGGV
                     SQTESRKL"
     gene            complement(13558..14070)
                     /locus_tag="ACHLAM_002316"
     CDS             complement(13558..14070)
                     /locus_tag="ACHLAM_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7853834.1"
                     /translation="MKKTMLSACFLLVAGNAVANDGTIHFTGSITDQTCTVDSGSQNL
                     NVDLGNVGQTALNGAAGMKASPTRFTLNLSGCPDTVTGANVKFDGTSDGVNQNLLALD
                     SGTGIATGVGIEIADKNGAVIPLHTASMDYPLAAGSNTLDFVARYVSTGTAVTTGTAN
                     GTSQFTINYK"
     gene            complement(14438..15028)
                     /locus_tag="ACHLAM_002317"
     CDS             complement(14438..15028)
                     /locus_tag="ACHLAM_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFO7853835.1"
                     /translation="MPEKRKHLTRHEVGQILLAADRGIHFERDYCLIQMCFLHGLRVS
                     ELCGMRLSDIDLIGRSVYVRRLKNSLSTQHPLFDNELPALMRWLAVRNRWRDADSDWL
                     FLSQKGGALSRHQVRLLLKRYGELAGVSISAYPHMLRHGCGYALADLGRDTRLIQDYL
                     GHRNIRHTVIYTATNTQRFMNVWGNVRENHTISARM"
     gene            15241..16164
                     /locus_tag="ACHLAM_002318"
     CDS             15241..16164
                     /locus_tag="ACHLAM_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO7853836.1"
                     /translation="MRAQYILLLLCAAFPLRAITTPEIAASALTPDCVKYRVVGLCYW
                     LYCTPFGCTVRTSVKVSHFRPDLVVSAYSNTGQNPWAEMSLLSPPLPGVAEGGGDTNP
                     RSASQHSKVRFKNADAIGFPAGDELAKFFTQFGYICSPSSQPFQPYFLSQPDTLAWRS
                     GVPEMTYPEALTPGMREVGQNGDMWGNIYPRAGAINQTHDYKAAAVIAQRAADLVTRT
                     GQPHLYTPLTASSRAGYWPPSPVIEGDSDNHRWQMLTPKKSAACSVFPDGSATDTYAN
                     KLAEDGAYAWTLWRPYKCCPRRGQTFLGSTG"
     gene            16176..17537
                     /locus_tag="ACHLAM_002319"
     CDS             16176..17537
                     /locus_tag="ACHLAM_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO7853837.1"
                     /translation="MRALFLVLLFFAGVCVAADDYSFRTRGAVDDLLYYQIGGGEAVM
                     PPPVRRSPSPLSLGLGWNADMMCGNFDIDTTVRNQLNGITDGFQQLMGNVIQSATGAV
                     ASLPAMIIQRANPQLYDLLTNGVLQGRLDFDKSLLNCQKMAEKMTDLAADSAWFSGAK
                     AENYQSLAASDNDAIRTDQKAAKEAAEKGKRWVGGEKRGGKGQLPIKVVHDATAAGWN
                     ILNQQPATSTTSLSSSECDGELCSTWTSPEEAAGWMTRVVGEQTISVAQVTDDPDARS
                     GAQAGIGLHPLIDEEKAKILPLLTDLVNGSQKPGFDNLAKASGGSMQLTRGVVEALRD
                     DPDVAVLTERLAGELALARVTEQALLARRTLLAGMREPNIANVKEAQESLGKTTAQLD
                     EELNQLKLELDFRQALTRNTASQILQRKQQRDQLQGQVVEVPDDSDSRLHNLNNPQPD
                     SPR"
     gene            17534..19036
                     /locus_tag="ACHLAM_002320"
     CDS             17534..19036
                     /locus_tag="ACHLAM_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO7853838.1"
                     /translation="MTANSYFEYVLTLLGWLVNNGVWQTLTATGLFAVPLLAKLVALW
                     LKARGQGADEGNVARLLVVWVEHSLYSALLVIFFAGVPFLNVDINTLTYDSQRTKQCG
                     WSVVQPGDSGYAPLINELSGQSASVPVWWYFTHALGKGITSAAVAVLPCKPDLRQLRF
                     EVQHTRLADPALAQELQDFVNDCYGPSRARLKRLSSDISEEQSREVDWVGSSFYLATL
                     GYYDRDHSRSPRAAWPYDASRDDGLSDTGGGGYPACKEWWSDGDTGLKNRLLKQVDTT
                     LWQQLQKLGQSKEEYEEAVLRSLVSPRNMQVSQGGLVYNGFGGSAGDNLSQTQLLSRV
                     GGIIGAGLSSYALFPAFDNVRQALPMVQAFLEMALVICIPVILLFSAWDLKTVITLSF
                     VQFALFFLTFWWELARWLDNWLMQMMYDSDTHSYFNLWGLQNTSDDLIVNIIMGVMFL
                     VLPAFWLGALTWAGIRIGAAVAGGMGSAVGDIRSAGEQVGKMIVSKTRIP"
     gene            complement(19181..19381)
                     /gene="glgS"
                     /locus_tag="ACHLAM_002321"
     CDS             complement(19181..19381)
                     /gene="glgS"
                     /locus_tag="ACHLAM_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO7853839.1"
                     /translation="MNRQDINALKNFDFLARSFARMHALGQPVDIDAVTGNMSDEQQA
                     WFRERYDHYRKQAERARVIELR"
     gene            19715..20005
                     /locus_tag="ACHLAM_002322"
     CDS             19715..20005
                     /locus_tag="ACHLAM_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853840.1"
                     /translation="MSLFDKKPSLMPVVIAPDVWQSAVEFPADPQQVEERLGNLLLVV
                     LLTLRTAGTSRKKVTFTIYCQPPQGDMKVPVALPLSLNYHGHYVQVSMAEQA"
     gene            20071..20976
                     /locus_tag="ACHLAM_002323"
     CDS             20071..20976
                     /locus_tag="ACHLAM_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO7853841.1"
                     /translation="MDRSNDIYQKVTDQIIAELENGNVPWLRPWRDGEPPFPINALSG
                     RPYHGINVPLLWNSADRQSFASDRWLTFHQVSALGGQVRRGEKSSLAVLYLPRTQEET
                     DALGQPLLDKDGEPKVRHFGIIREFWLFNLSQCDGLPQALSEPFTRPATPVEMAEAIT
                     MNSGVTLRHRRQSKAYYKPATDLVMMPHPQQFESSEAYYATLLHELTHATGHASRLNR
                     PGIAGAERTEALYAAEELVEEMGSAFLCAHCGIQGRLQHASYIGYWLDILRQDKRAVI
                     RASGMARHASEWLLNAVPQPCQLSA"
     gene            21042..21413
                     /locus_tag="ACHLAM_002324"
     CDS             21042..21413
                     /locus_tag="ACHLAM_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853842.1"
                     /translation="MGLDLFFFERKKNAPDESQYARVGYFRKVNPLLKWVDKHVGLVR
                     NQVLLEVTKANLQALLSDLQSLTPDNCGAVYPTIDGFFYGYTDYGEHYWKDVRTVTLW
                     VKETLETFDFERDRLMFYAWW"
     gene            21587..23176
                     /gene="mobH"
                     /locus_tag="ACHLAM_002325"
     CDS             21587..23176
                     /gene="mobH"
                     /locus_tag="ACHLAM_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFO7853843.1"
                     /translation="MLKWLKRPSQSQPPTPDATLPEGWFAPQSAEQLLATPLRQSCLH
                     QLRQLTPLPDTLFDSWILAAIHRFAALVQLLPASQSHHHSTPGGLLDHSLEVACNAIR
                     LRQGYLLPPEAGAEEQARQASAWTVAVVCVALLHDTGKIAVDMEIHQRDGTRWHSWQG
                     YLAQPYRWQYQSHGRDYHLHPAATAMLINQLIPSPLLDWLAGYPAAFASLIYQLTGHY
                     ERSGALAELVQQADKASVARYLGGDIKTALAQKPSSFPQQLLTALRSLVQNEYRLNNP
                     ECGSDGWLTEDALWLISKTTADRVRAWLLQHGVSSVPENNNRLFDELLTHQLIVANED
                     KAIWRCIIRSDKGWSPVEPLTLLRLTPSVIWGQDDQRPACFDGDVTPVIEAEPAPELS
                     APTQPLPEIADDPRLGDNNNFLQWLKNHTSPSATVNTRNGKVHVVENHLFLISPGIFK
                     TYAAETTGDSGDGWKLAQRMFQESGLALRCNDDSFIWICEVRVPQKKSSQLKGFLMED
                     PKQVLGEKVPVNNPWLKLLRE"
     gene            complement(23232..23885)
                     /locus_tag="ACHLAM_002326"
     CDS             complement(23232..23885)
                     /locus_tag="ACHLAM_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7853844.1"
                     /translation="MKKKLPDQLSRECIIAAMRAYDEGVRHQFSESRLYDVVFEGRSY
                     PPKAIVGIAASNMTGVEFSPEDFSGGIKSKCVKLLTDQGFQIVEKHLGNEQPAISIFP
                     DELPPQSVFTEGAATQVTVNRYERDRKARQAALAWHGCLCKVCGIDMMKVYGEIAKGF
                     IHIHHLIPLSDIKENYSLDPKKDFIPVCPNCHAMLHRRNPPFSPEELKSIIEYHAAL"
     gene            complement(23997..24263)
                     /locus_tag="ACHLAM_002327"
     CDS             complement(23997..24263)
                     /locus_tag="ACHLAM_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005289972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853845.1"
                     /translation="MSPRTHRQLVSVEVMWPAQTLPLPLQQVVEALNQGETPDQIIIR
                     MNQRGLLAWREDASAQDTHDIFQVRLDNQNEARFLCRYVTLPLH"
     gene            complement(24390..24731)
                     /locus_tag="ACHLAM_002328"
     CDS             complement(24390..24731)
                     /locus_tag="ACHLAM_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012776626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7853846.1"
                     /translation="MNVTSPKMLSQAIRDARKQRNLTQRETAASMGMKQSTVSEFENH
                     PEGTSLDTLFKLLAALDLKLQVVSWRGKKTLLMAGSRSGDLWKYEQKGHGAIRTEPPF
                     SLAYHLDFQST"
     gene            complement(25142..>25618)
                     /locus_tag="ACHLAM_002329"
     CDS             complement(25142..>25618)
                     /locus_tag="ACHLAM_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7853847.1"
                     /translation="TAPLVPGTNQLLAEFGERLKLARLRRKLTAKRVAERAGMSPMTL
                     RSLESGGTGVTIGAYLSVMQILGLEKDLNKLAAEDEQGRQLQDSRLINKTRVSRPAAI
                     SHTNRHTGNVIKNDAFDKQFSTSQEKSIDSEVKTEVVTSASLAALLKPIKKPRKGS"
BASE COUNT         5895 a         7058 c         6529 g         6136 t
ORIGIN      
        1 ctggagaaca caaacctaca aaattataaa actcaataac tctattgagc acccagccaa
       61 cctccagatt gaagatattc aaaatttatc aacaggcaga cctgttttat taggtgataa
      121 cttgcatgaa cttaatgagt ttatagagaa taccaggcaa caatttactc ctaacacaat
      181 aaattggtgg cgtaccccgt ttattgcttt gaataattgg attaaccaag aaacagtcaa
      241 tactattggt ggatacatcc aaatggtaac cgcgcaccca cttggtactc gaatatcatt
      301 tttgaccaat ttgaatgaaa atattatcag aacaagctat gcaggaatta acactactga
      361 gtcatttgga ccaactattg gtggttttat cctaatgcca atggatggga tgtcactccc
      421 agatgaaaac ggctgggatg ttggaaatca agttacccgt actgctgcgg atagagttgc
      481 tactaataga taaacctatc tttcaaagtt gaataattct ttgatgtcca tgtccaaccc
      541 ttttcctaaa acacttatca cctcaagggt tggattcctt actccccgct caataccgct
      601 cacataagta cgatcgagac cacacttatc cgcgaatgct tcctgagaca ggcccgactg
      661 gagtctgagt tttttgagat gtgcgccaaa ctgcgcctga atactggatg tatttttcat
      721 ccagataaaa tgaacttacg ttgtctatca gaccacggac tatgagtcac acttcgttga
      781 ttcggcgtcc ttttctgcgc catcgtcaaa cataaataaa tggctaagat ccatccccag
      841 accgtccgag attgcacaaa tgacaacaag cgtggggttc cttactcctc gctcaattcc
      901 actcatatag gtgcgatcga taccgcattt gtcggctaaa gcttcctggg ataggtccat
      961 ctcacggcgt agagccctga ttcttttacc cagtaccttt ttgattgatc ccgtcttttc
     1021 catctagcca gcatcaatct tcgttgatta tcagtccacg gactatgagt cacataaaaa
     1081 gcgtgctagc atacattcgt gactcatagt caacaataga ggtaaacgga atgactaaaa
     1141 aagactggca ccccgccgat atcattgccg ccttacacaa gcgcggcacc agtctggcga
     1201 aactctcgcg ggaagcggga ctggcctcgt caacgctggc gaatgccctg aaccgaccgt
     1261 ggccgaaagg tgagtggctg attgccattg cgctgggaaa gcatccgtca gaaatctggc
     1321 cggaacgtta cctcaatacg ggaaataaac gtgaaccccg taaaccgctt cacctcatga
     1381 caacaacaga aaactccggg acataacgac ccgcgctgtg agtggcagga cgcattaatc
     1441 aacgacacct gacaggagag ttgtttatgc acaccgaacc acacccttgc tccatcgctc
     1501 ttacaaccgg aggccacctt cacccggcct cccggactga aggctttatt cacctattca
     1561 ggctacatgc ctgctcacct acaccaggcc gtactctcat gcccgacaaa cgggagaacg
     1621 agccatgatg acctactgcg cacgcatcgt tgctatcggg ctgtttgtcg ccgacggact
     1681 ggcagacagg acgcttatca cctttgacag caacggaccg aaagactgcc tggactattc
     1741 cctgtcgctg gaaccctcat tccgcgaaga cgacctgctg atcctgcccg gcgatcggct
     1801 gctgctggcg agccatgact acctggtgac cgccgtgggt aaagaggcac agcagtcttt
     1861 gtttgaactt ggccacctga ccatggtgtt taacggcagc ctgaatgcct gccattcggg
     1921 agccgttcat ctctccggcc caatccctaa acttcacgac ctgtacggtg aactgctgat
     1981 tgaggaggga cggccataag cgctgccttg aatccatcca caacaacaaa agagaagaca
     2041 ttatggaact caacagaagt ctgaaagccc gtcacctgac gatgatttcc gtgggaggcg
     2101 tgattggtgc cggatttttc ctcggcgcag gcgcggccat tgcccgtacc gggccgtcgg
     2161 tggtgctggc ctaccttatc ggcggtgtca tcaccctgtt cattatgctg ttgcttgccg
     2221 aaatggcggt cgccaggccc gtctccggtt cctttgaatc ctatgcccgc accgcctttg
     2281 gcccatggtg tggctttatc accgggtgga cctactggct ggccttcctg attggcccgg
     2341 cgtcggaggc catcgctgca ggcaccttcc tgaacctgtg gtttcccgga gtccccgtct
     2401 ggctgttttg cctggtgatc gcttcgtcgc tgactgccat caacatggtc ggcgttcatt
     2461 ttttcggtga agtggagttc tggctgagcc tggtgaaagt cgtggcgctg ctggccttta
     2521 tcgtcgcggg ctgctacagc ctgggactgg ataacgcctc acccgccagc atcagtcatc
     2581 cgaccgacag cggcagcttt tttgctggcg ggctgcccgg atttctcggc gcgatgttaa
     2641 tggtgatttt ctctttcggc gggacagagg ccatcggcac cgccgcgggt gaaagcgaga
     2701 acccacagcg tgatattcct cgtacgctgc gtggcaccgt ggtgcggatc ctgttgctgt
     2761 atgtcggctc attgaccgtt ctgctgctcg ttctcccctg gcagcaggca ggtgtcagca
     2821 gcagcccgta tgtcgaagct tccggcattc tcggtggcca tatggcgcgg catatcatga
     2881 actttgtggt gctgaccgcc gcgctctcct gcatcgatac cggggtttac gccacctcgc
     2941 ggatgctgca cgccatggcg tctgacggct atttcccggc ctggtttgcc cgcctgcacc
     3001 cggcacataa aacaccggat aacgccattc ttgccagcag cctggtgctg tttaccggcg
     3061 tcatcatcgc cattctctcg ccagatgcgt atgtcatcct cgccgggatt tccggattcg
     3121 gctttctgtt cacctggctg atgattgcct taagccagcc caccctgcac cgaatagccc
     3181 tgcacgacgg aacgctgaaa tacaaggctc cggccgcccg ctacatccag ccactcaccg
     3241 tggggctgat tgcgctgatc atggccgggc aatttttcac cgacggcggc ggcattaccc
     3301 tggttaccgt cctgatctgg ctggccctcg ccagcgcgta ctacatcttt gtcgtccgaa
     3361 aaaacaggag gctgtccgat gaaaaaagtc atgctcaatg aactgactgg ccgggaaatc
     3421 cgcgagctgt tgcagtctgg taaggaactc actgctatcg tcaacttcgg cagttgtgag
     3481 aaccacgccg atcacctgcc actcggcccc gatatgtttg tacccgaaat cgtcgcctgc
     3541 cgggtggcgc agcgggtgga taataccctt gtgctgccct gcacgccgtt cggcacatcc
     3601 gttcactaca atgcctatcc actggcggtc accctacgct acgagaccaa tatcgcgctg
     3661 gcggaagata tcttcatcag cctgctgcac cacggtattc gccggatcct ggtgattaac
     3721 ggccatgacg gtaatattcc ggcgattgaa atcgccgccc gcaatgtgaa ggcccgacat
     3781 acgcaggcag cgattgtcgt ggttccggcg tggtgggatc tggtgggaca tcatctcggg
     3841 aagggatttt tcgactcatg ggataacacc ggactaggac acggcggcga gggcgaaacg
     3901 tccgtcatga tggcgctgat tccggagctg gtggaactga aatatgcgca gaaacagatc
     3961 ccggtggagg tgttcgctga ttatggcgtg aaccggatct gggatatcgg ggaaatcagc
     4021 gccaccggag caaccggcga tccgcgctgc gccacggcgg aaaaaggcca gcggatcctc
     4081 gcctgtctgg tggatgtgct ttgcgcgttg ctgagaaagc tggatgcagc taactggcaa
     4141 tatgcgttaa ataaagacgc aaacatgtaa tcgatatgct cgggcaacct gtggagtggc
     4201 ttttgccttt ccacaggtta cccggaagcg atatcccaac cgggtaaccg attggtgtta
     4261 gcccagaatc tattatactt caatgagtaa tattattgct gagatgaatg acagttcagg
     4321 tttagtcacc ttaccaccaa caaattctgc acatttactc ctgagatttg ctaattcatc
     4381 ttttaatttt tcgttgctgt gttgcttaag atatatttgt cctcttagct caacttcagt
     4441 acttgccttc aaaaattcct ttcggctttg ataggtggga tcgttatcga gattttttgg
     4501 tatagttatt aaacatttct gaaatgcgat taaagcttcg attaggagaa gtctagagtt
     4561 ttctttgtat tgaggcttcc actgatgcaa cgaccgccaa gcaagtagta aacctatcgt
     4621 tgtcacaaca aacgtcaaac aggcagcaat agcagcaacc cccgcccatt ttgcagcttg
     4681 ttgattagcc accaatgtgc tcaagcttat gaaatccgca tctatatgca ctatttttac
     4741 ctcagtaaga aaagtggatg taaatctact ttttcatttt tgaaacagca acttgatttt
     4801 aagccatcgt cctttgagca agattttccg caccagttga atcacgtcaa ccaccagcac
     4861 gccctcctct ctcagtgcat cccatcataa tggcaatctg taaaacagca gtccttgctt
     4921 taaaaatgtg tcattcctca attgtctggc caagcttatt caaatcatcc agccccgaga
     4981 tttgcttgag cacagaaagg tactcctgat ggttatcata aagccagtta agtacctgac
     5041 cgttcgccag cagacgttca atgtgatttt taatgataac cagccgaagg ttattctcag
     5101 catattcatc ctgaagatgc tgcgtttcca cctgaacgga agccatttct ttttccagtc
     5161 tttcgagatt ttttctgata tctgtttctc tgctggcatc ttttttaggc gctacaagat
     5221 gttcaggggc aacggcatgc agcatcgaca tcgcaaattt gcgggtgaaa ttattaatac
     5281 tgatcatggt gttaaccatt tctatctgct tatagaactt tagttttttc aacacagaga
     5341 aaatggtctg cggtacattt ttttctgcca gtaacgccat gacttcaggg gcaatgccat
     5401 cagaaatttt cagtttacca atcagcgtat ctggcgtgac acccagtacg gcgcatagtt
     5461 tttcaactga aacgccagat tcaaccgctt taagcagcat gttttgggcc tgcacaatgg
     5521 taaggcgact aacacgtttg ttatatgaat atgtatcctc cacaggggat atcagacaat
     5581 gagcagtagt tattccgagt tctttcaaaa cttcaacgcg aagatgtcca tccagaatga
     5641 ctttatcatt ggcgtcattg atataaacca caatgggttc aattaaccca atgtcatcga
     5701 cagaggcttt aatttgtttg tacttcgtac tgaatttaac attaaccggc agcgttctgg
     5761 catacaccag tgattccacc ttaaaggtat aaaatcggga gtcaaaacat tgtgaaatca
     5821 tcttatttct cccccgatac atcactgcca tccagtaaag aaggaatatc gtggatatta
     5881 ttactgtgca gcaatgtaac aaaatcatta tctttaagta aataaccaaa tatttgcttt
     5941 actgccagaa ttttctcttt aaccattgtg gatttcagca gaatggcttt ttgctcatta
     6001 atgttttcct ggtagacatt agccagttct tccggggtca gttttttctg ttttttccca
     6061 ggaaaaaaag ccgtactaat cgtaccttta tcgcctttct tacgttgttc aagtatgcgc
     6121 ctgatcttat taatttgccc acccttgata tgtccttctt tcagggcgac cgtcagcagt
     6181 tcctgagttt cctcatctcc tgaccgggat atttccacag caagatacaa gggaatatcg
     6241 ccagtctcaa atgcccgcaa aagatgcttc tctccggatg acaacagagt gatcacccca
     6301 tttacccaac tcgaattata cccaagccgc aagcctattt cacgatcggt caggccactg
     6361 gtcttcattc ttctgatatc ttccattagc tctgccgccc ggggattacg tcttgcgatg
     6421 ttttcagcca ggctcatcag gtgcgctgtc tcttcatcaa cctgcttgat gattgcagga
     6481 attttcgttt caccatactc aatatacgct tcaagtcgtc cctgaccaca aatcagggca
     6541 tatttatagt tttcattatt cactgggcgc acgctaacag gccgtttcag accaatggtt
     6601 tttatgttct cttttatttc attatgaatg aatttattcc ttttccgtgg atttgcgata
     6661 taaatatcac tgatatcgac aagttctatc tgttcatcaa aagatttcat tatttactct
     6721 ccagaccagt tcgttttaca agattttcca gacatgacaa atcatcaaat cgatatagtt
     6781 cgaggataaa accattgttc tcatgaagcg ttaaatcgtt atacaacaca tcaatagccg
     6841 gaaacataaa gtaatcaacc acggagttat ttgtgctatc caaccgtgga aagatcatga
     6901 catcacaata atgggataca ttaaatttta tcttccaacg aagcttacct gacgacaaac
     6961 gttggcagcg aaccagatta gcggaaacaa aaaactcatc attaatatat aatgaaccat
     7021 cctgatgcat ctctgcccaa ccgccatatc ggtatatatc ctgcaccaaa ttatccatga
     7081 aagtgagatg gcattctgca atctgctttt ttatttcaag ataagagtaa tcatggcgcg
     7141 ggtgataacc aataagggtg taagccctga ctaacccacc aaaccgtgaa cgaaaaatgc
     7201 tgctcgatgg aaacagttcc tcttcatcaa tcacaatccc ggataatctg ccacgaagct
     7261 tcagcagatg gcgtagttta tccagcagtt catcatcact gtactgaacg gccctggctt
     7321 caataatcct tttagccgcc tcaaatttct ctactgatac cacagggtcc cacgcgccgt
     7381 cacatctgat ccactgatcc tgaggattcc tgacacttcg ggtcttcaat cgtccggagg
     7441 ttctgttata aacattattg ccaatatatt tttcattggt cagtatctga tgaattttac
     7501 atcggctcca ctggagccca ttctcggcgg ggatgttctt ccggttcagt tctgaagcaa
     7561 taacaaactc aggcttgccc tcatcaataa acatgtcata gattttattg actattacca
     7621 cctcttccgc gggccccggg atcaatatta ctctgtcagt ttgcaggctt ttcctttgcc
     7681 ccggcagaag catgcctttc ggattagtat gttcatcaat taataatctc cgaatgccat
     7741 agccaggcat accaccttga taataacccc gccgaacgag gtttgcctgg ccaagaaaaa
     7801 ctttttgtga aaggttttta ctgtaagttg cggcgccata gcgggagtaa gataatccca
     7861 gcataaacaa ttcggtctgt tcttccgaga aaggctccgc gcaataaata atgcgcacac
     7921 cattacgctt cagtagccac gagtaatgag cagcctcttc aatatcctga aaacgaccaa
     7981 accgactgac atcgtaaaca agaattaacg agacttttat tattccgttt tcaacatcat
     8041 ataacagcga acgcaggcca ggcctgttat ccaggctcag cccacttcgg ccagcgtcgt
     8101 cataggtata tttaatggta atacagtgtt cttttgcata cttctcgata tattcccgct
     8161 ggttctccgg cgaatatttc tgatgctcgg tagacattct taaatattga gcagcaatcc
     8221 tgttcccggc gacaatatcc gacatagaat attccatatg attacgcgct atttataact
     8281 catcgtaaac gtcgccgtac cgtttgccgc acctggcgtg atcgagcctg cagtctggta
     8341 atatcgcgcc ttaagctgca cgatctcgtc accatatacc gactgccagt aaaaccgttc
     8401 ctgaccgtac tggacagcgg catcatccgg gcggtaatga agttgtatcc ccacgccaga
     8461 cgccccgccg ctatcaatca gcatgacgcc ttgcgaactg gcggaagcat ccgcagtggc
     8521 atcgatacgt acgtttattc tcgccccgac atcgcagctc aaaccaatat caaagctcac
     8581 ccagtcggtt gaagatcccg caccactgaa ttcgctttta tcgtgatccc ccatcgggac
     8641 atcaacagag ggcgtggtca cagtacaagc ttctgatttc actgtgttca tcccgttcaa
     8701 cgtgacgttg gcaaaataaa actgattcac taccgaagcg cgcgccagca caccgtccgt
     8761 taatgtaccg ctggtgatat ccccggtttt gataagctcg cccttgatgc catcaccggg
     8821 aatggatacc caggtgccgc cagcgttgta ggcgtagctg gtttcatagg gcaatgtttt
     8881 acctgtcgag gccgcactca gacgtaatcc tacccctggc aggttggtgt tgtagacacc
     8941 attcccatag ccactcagcg tcccccattg cgtcagttcc cagcgggcgg tccaggcctc
     9001 ccgcttacac ccggcaatgc cgtcgccatt gttgtatgcc cctgtaatgg cagtggcaat
     9061 cgtgctgccg acctgtgcat cccgctggat aaccacgttg ccgaaattca ggtaacccga
     9121 tacttctgat gtcacaccac cgtagaacga acaggtggcc tgaacctccg gcaggacggc
     9181 tatcatcaac agcaggaaga aaagaatttt tctcatctca acactccggc gacgtcattt
     9241 ataggtcagc gtgaaggtcg ccgtggcatt ggcaacacct gcagtaatcg tggctgcggt
     9301 ctggtaatac cgcgccttaa gctgcactgt ctcagtaccg ccattgggag aggtatagta
     9361 atagcgtgac tgaccaaact gtacggcgct gttatccggt gcaaagaaca actggacacc
     9421 gatgcccgta gccgccatgt ctccgggcgc gctgtccagc ttcatcacgc cagccacgtt
     9481 ggacggatcc tgtacggcat ctatctgaac gttgatacgg gcacttttat cgcagtccag
     9541 cccgatgtta aacgcctgcc agccggttcc cgttcccggc ccggagaact ccgctttttg
     9601 atgctgcccc agcggcacat tgacatcagg cgtggtcact gagcaggcga tggtggtgat
     9661 ttgcccaccg gaaaatgtaa tgggaatttc agtcgaccac tgcccgttct cccttgtccc
     9721 cgcgataaaa cgccccaggt tgccgctcag cgtaccgggc gtaatgtcgc cggtttttat
     9781 cagccggatc cgcgcctgat cgttaatgga gggatggtag ccaacgctgt ggcttatctg
     9841 gagcgtgaat acctgcgact gcccgctggc gataccgatc cagttttccg gtaacggatt
     9901 gctgtcgacc gaggagcggc cttcaaacac aaatcccaca cctgcgacat tactgttaaa
     9961 caccgcctca ccattccagg tgacgctggc gcgggagccc gtgtttttga tgcctgcatt
    10021 gatgacgtat tgtggcgttg catcataatt gcagtcctga taggccatcc cctggttcga
    10081 caccagccag tcgctaatgg gttcccccac cacagcatcc cgctggacca ccaccgtacc
    10141 ggggctgatg gagaaactca ccgaagacgt gcaaaacgcc cacgacgcct ggcagaacag
    10201 gatccccgcc agcagaatca tcccgcgcca tactctcatt ttgccacctc cctgcattcc
    10261 tggttcacca ggatcactga cggctggttt tcctgttccg gtaaacggta atgcaccacg
    10321 cactgttgtg ctccgttcca gttaaccgca atatcgcctt cctccggcac gccagtcaga
    10381 tagacctggc catcatcgcc gacaataccg ctgccgccct ctttcagctt cgcctctgcg
    10441 ccaaagggga ctggcagccc acggtacagc agcgtcatga gcacacgccg cccgacacgg
    10501 gtgttaaaat ccgccatcac cacggcaccc tgcgtggggg tcacggtctg tacggcagtg
    10561 tcggtatcca cttcgtcgcc aagcgtgctg gtgtcgagcg caatacggtt tttcttgtac
    10621 gggttcacat acggcactac cgcataaccg cgccagtcgg tgtaaacccc ggtgttgttc
    10681 tgtaccttcg tatcatccgc accgggcgct ttcacgatcg ccagcgactg gccgagcggc
    10741 tgcgaaagcg tgatgccatg cggatgcgcg acaataccgc ccttcagccc gtagttaacc
    10801 tgctgcgtgt cgtcgtcata gttgtagcct ccggtgattt ccccctgccc gcctttatag
    10861 tcggcgttga tattgccgga gccgcccaca ccatggttgg tgtagctctg ctggaggctg
    10921 tagctcaggt tgttgtcggc aagcgccgta ccgttcagcc ctacctgctg gcgggtatcg
    10981 ccgtgtttcg cggtgttgac gctgtagctc gcccaggcgt tcggcataaa gcggctgagc
    11041 ggcacctgga tgttgaacgc cagttgctgg tcggcaggct gcgtgctctc cgggtagtcg
    11101 ctgtaactcc aggtaacgct gtagctgata ccgtcgatat tcccgttcca gcccgcgctc
    11161 aacgtgcgtt cgtagccgtc ctcctgccag taatcctgct ggtagccgga gataaaaaac
    11221 gagccatagc taccgatact ctgactgaga tctaactgga ggcgctggcg tttgttgtac
    11281 gtcatctgcc agccgttatc atcatcagcc ctcaggtcgt tggcttcctg gaaggtgtaa
    11341 aatccggcgg tggagtagcg atatgccgcc agcgtgaagc tggtgtcggt ggtctgaaaa
    11401 tccttcgaat actgcacccg gtacgactgc ccgttgtggg tttcatcgtt attgagcgtg
    11461 gtatgggcgg cggtgatatc ggctgacagc gaaccgagtt caccgaggcc aaaaccgagc
    11521 cctgcggcgc cagactgata atcgtccgag tacagcgtgc cgccatagac ggtgatagca
    11581 tgcggcagac cgtaaatcgc agtgacctgg ccgaactgcg gctcgtcgct gtcctcattg
    11641 ccgctgcggt atttcccggc ggtcagggca tatttcaggc ggccttcacg ttgcataatc
    11701 ggcaccgccg agaacggctg gacgaaggtg cgctcgctgc cgtcggcctc tttgatggtc
    11761 acttccaggt caccgctgcc cgaggtcgga tacagatcgg tgatggtaaa cgcacccggc
    11821 gcgacgtagc tctggtagat gatgtagccg ttctgcttaa tggtcacctg agcgttagac
    11881 tgggcgatac cgcggatcac cggggcgaac ccgcgcaggc tgtccggcag catgttgtcg
    11941 tcagatgcca actgcgcgcc gcgaaactgg acgctgtcga agatgtccga cggcgtgtag
    12001 ctgtcgccga gcgtcagttg tcccttcagg cgctggatat cccgttgcag gtacgtgttt
    12061 actgaatccc agtggctacc attatcttcg tcataattcc aggtcgagta gttgcgtagc
    12121 cgccacgcgc cgaggttgat accgctttgc aggttcgcgt agtagttatc atccgaaccg
    12181 ttttgtccat cgcgccaggt gttgctgccg ctgaggttgt agttcagcag tgcggcgggg
    12241 acaccttcgt cccattcgga cggatccacg tatcctctgg cgctggcatt cattgccgcc
    12301 tggggaatac tgacgttcaa ctgcaattta ctgaagcgca gttcagtgtt ggccatcggg
    12361 atatagcggc caatatcatc cagtggttga tccggcggca gtgctgccag tgccgggaag
    12421 acgtccgttt tgacgcccca ggctttcagc atcgcaggcg ttatctgcgg gctgagtttc
    12481 tttttggctc cgtcaataaa agcaatatcc tgtgcgtcgc tctgcttttc accgttcacg
    12541 aagaccgtta cgcggtaggt accaggtagc tgaccaccgg attccgcaaa ggtggataaa
    12601 tccgccaccg gctggccggg gttatcaatc tccagcgcgt gggggttaaa cacctcacgc
    12661 gcctcggcat tacgcaaata gccagacaaa agcactattg ccaacaacag cgtcagcagg
    12721 attatcatca gacgtggcgt cgggttattc atcacgtcct ccttcacagc ttacggcttt
    12781 cggtctggct gacaccgcca tagtcgctga tggcctgcca ggtcaccgcg cttgcagtcg
    12841 ctgacggtag cgtgaaactc acactgcctt tcggcggcac catatccgcc tctttaatct
    12901 cctgcccgtc gaccttcagg gtgaacagcg tgacgtaata cggtgtggga ttgctgacgg
    12961 taagatggtt accgctgtgg gcaacgctca actggtgata ggcactctcc ggctgccctt
    13021 ccaggcctgc cggacggtaa aacagtttca gccgcgactt gaccactacc tgcaacacgt
    13081 tggtgcgcga atggctgtcg tcggtagcgg ggatcgattt cacgttcagc cagaacaccg
    13141 actccctgtc ctgcggcagg ttgcctccgg tgcgcacaat gcgcagcatg ttctcttcgc
    13201 cgggattaat gcgaaacaac ggtggcgtga cgataaacgg cgttttgccc ccctgttcgc
    13261 cactgtctac ccagctttgc accaggtaga cgccggattt ctccgggttg cgtaccgagg
    13321 cggccgtctc ttttttatcc ccgtcataaa tcacgcgcgt accgccaatc acgatcccgg
    13381 cctgcgcctg tacgctcagg aaagccagca acagtgccag caccacgtct gcatacctgt
    13441 tcatcttctt gttctccgga cgaaaaatac gcatccctgc gaaacagtcc ctgttatgcg
    13501 gcggtacacc aggtcgggta ggatgggaga ggtggtgaac cccgcgacgg cacagcgtta
    13561 tttgtagtta atggtgaact gcgaggtacc gttagctgta cctgtggtta ccgcggtacc
    13621 ggtggaaaca tagcgggcga cgaagtccag cgtattgctg cctgccgcga gggggtaatc
    13681 cattgaggcc gtatgcagag gaatgactgc accattttta tcggcgattt cgataccaac
    13741 gccggtcgca atcccggtac cgctgtccag cgccagcagg ttctggttta cgccatcaga
    13801 cgtcccgtca aacttcacgt tcgccccggt aacggtatca gggcaaccac tcaggttgag
    13861 cgtgaagcgc gtcggggagg ctttcatccc ggccgcaccg ttcagtgcgg tttgccctac
    13921 attgcccaga tcgacgttca ggttttgcga accgctgtcc acggtgcatg tctggtcggt
    13981 aatactgccg gtaaaatgga tggtgccatc attggcgacc gcattcccgg ccaccagtaa
    14041 aaaacacgcg cttaacatcg tttttttcat acgtttctcc ttgatgaaag atcactcagg
    14101 gctcaaccct gattcactct ggtgacgaga aaacccgcgt aagactttcc cgttagtgcc
    14161 agagtggtga gttcaggaga aaatgtggtg gacgaagggg gtgaaaaata aaatgtcagt
    14221 ggaagtgaag tatggctttc aaaatggcat aaaaccgtaa tgcggtgagt gagtaaaaac
    14281 tggcaacgtg aaccacttaa gcacgttgcc agccaggacc agagagagac tacatatttg
    14341 ttacattaaa tattatttta aaaacaacaa gatagtccgt taccaaaagg ggtctcagaa
    14401 aaattttgac agtttgctaa atttttctga tactacttta cattctggca ctaattgtgt
    14461 ggttttctct cacgtttccc cagacattca taaacctctg cgtatttgtc gccgtataaa
    14521 tcaccgtatg ccggatgttc cggtgcccca gataatcctg tatcagacgg gtgtcgcgcc
    14581 ccagatctgc cagcgcgtaa ccgcaaccgt gtctcagcat atgcggataa gcggaaatgc
    14641 tgacacctgc cagttctccg taacgtttga gtaacaatct aacctgatgg cgcgacagcg
    14701 caccaccctt ctgcgacagg aacaaccagt ctgaatccgc atcccgccag cggttgcgta
    14761 ccgccagcca gcgcatcagc gcaggcagtt cattatcaaa cagcggatgc tgcgtggaga
    14821 gactgttttt caaccgccgg acatacaccg agcgcccgat cagatcgata tcagacaggc
    14881 gcatcccgca cagttcactg acccgcaggc cgtgtaaaaa acacatctga atgaggcaat
    14941 aatcgcgctc aaaatggatc cctctgtctg ctgcaagcaa tatctgtccc acttcatgac
    15001 gagtgagatg ctttcgtttt tcaggcatca gccttgctcc ttatcaaaat aaaaaatgaa
    15061 aaaaaatcag gccagaactg ccttcacagt ccaggcctga accagagaat accaccagaa
    15121 acaacgtctg gtgatgatgt aaaactcaac aattttctgt aacggcttcc agtttcatac
    15181 cgttgatgaa cagatattgc ggtacacccg acagaagaaa tatctgctgt cggaggcgcg
    15241 atgcgagccc aatacatcct tctgctgctg tgcgctgctt tccccctcag ggcgattact
    15301 acgccggaga tcgcagcctc tgcgcttacg ccggactgtg tgaaataccg ggtggtcggc
    15361 ctgtgctact ggctgtactg cacgccgttc ggctgtacgg tacgcacgtc ggtgaaggtc
    15421 agccatttcc gcccggatct ggtggtgtcg gcttacagca acaccgggca gaatccgtgg
    15481 gcggagatgt cgttactcag cccaccgttg cccggtgttg ccgaaggcgg cggcgatacg
    15541 aatcctcgtt ctgccagcca gcacagcaag gtccgcttta agaatgccga tgccatcggc
    15601 ttcccggcgg gtgatgaact ggcaaaattt ttcacccagt ttggctatat ctgctcacct
    15661 tcatcgcagc cttttcaacc gtactttctg agtcaacccg atacgctggc gtggcgcagc
    15721 ggtgtgccgg aaatgacata cccggaggcg ctgacgccgg gcatgcggga agtcggtcag
    15781 aacggcgata tgtggggcaa tatctacccg cgagccggag ccatcaacca gacgcacgac
    15841 tataaagccg ccgccgttat cgcccagcgc gcggccgatc tggtgacccg taccgggcaa
    15901 ccgcatctct acacgccgct gaccgcctca tcgcgcgccg ggtactggcc gccctccccg
    15961 gttatcgaag gcgacagcga taaccatcgc tggcaaatgc tcacgccgaa aaaatccgcc
    16021 gcctgttcgg tgttcccgga tggcagtgcc actgacacct acgccaacaa actggcggag
    16081 gatggcgcgt acgcctggac gctgtggcgg ccctataaat gctgtccgcg ccgggggcaa
    16141 acctttctcg gcagtacggg ttaacggagg cggtgatgag agcccttttc ctggtcttat
    16201 tgtttttcgc cggggtttgc gtggcggcag acgactacag tttccgcacg cgaggcgcgg
    16261 ttgacgacct gctttactac cagattggcg gcggtgaagc ggtcatgccg cctccggtac
    16321 gccgtagccc ctcgccgctt agcttaggtc ttggctggaa cgccgatatg atgtgcggca
    16381 atttcgacat cgacaccacc gtgcgcaacc agctcaacgg catcaccgac ggcttccagc
    16441 aactgatggg caacgttatc cagagtgcca ccggggcggt cgccagcctg cccgcgatga
    16501 ttatccagcg cgccaatccg cagctttatg atctgctgac caacggcgtg ctgcaggggc
    16561 ggctggattt tgataagtct ctgctgaact gccagaaaat ggcggagaag atgaccgatt
    16621 tggcggccga ttccgcctgg ttttccggag ccaaagcgga gaactaccag tccctcgccg
    16681 ccagcgataa cgatgccatc cgcaccgacc agaaagcggc aaaagaggcc gccgaaaagg
    16741 gtaaacgctg ggtgggcggt gaaaagcgcg gcggcaaagg ccagctgccg attaaggtag
    16801 tccatgacgc caccgccgca ggatggaata tcctcaacca gcagcctgcc accagcacta
    16861 cttctcttag ctcctctgaa tgcgacgggg aactttgtag cacctggaca tcccctgaag
    16921 aggccgccgg gtggatgacc cgcgtcgttg gcgagcagac catcagcgtg gcgcaggtca
    16981 ccgacgatcc ggatgcgcgt tccggcgcgc aggcggggat cggcctgcat ccgctgatcg
    17041 acgaagaaaa ggcgaagatc ctgcccctcc tgacagatct ggttaatggc agtcagaaac
    17101 ccggcttcga caatttggcg aaagcttccg gcggttctat gcaactgacc cgcggcgtgg
    17161 tcgaggcgct gcgcgacgat ccggacgttg ccgtcctcac cgaacggctg gcgggagaac
    17221 tggcgctggc ccgcgtcacc gagcaggcgt tgctggcccg gcgtacgctg ctcgcaggta
    17281 tgcgtgaacc caatatcgcc aacgtcaaag aggcgcagga gagcctgggc aaaaccaccg
    17341 cccaactgga tgaagagctg aaccagttga agctggaact cgatttccgc caggcactga
    17401 cgcgcaatac cgccagccag atcctgcaac gcaaacagca gcgcgaccag cttcagggac
    17461 aggtggtgga ggtgccggac gacagcgaca gccgtctgca caacctcaat aacccgcagc
    17521 cggacagccc gcgatgaccg ccaacagcta tttcgagtac gtgctcaccc tgctcggctg
    17581 gctggtgaat aacggcgtgt ggcagacgct gaccgccacc gggctgttcg ccgtgcccct
    17641 gctggcaaaa ctggttgccc tgtggctgaa agcgcgcggc cagggggccg atgaaggcaa
    17701 cgtcgcccgc ctgctggtcg tctgggtgga gcactcgctc tacagcgcac tgctggtgat
    17761 cttcttcgcg ggtgtgccgt tcctcaatgt cgatatcaac accctcacct acgacagcca
    17821 gcgcacaaaa cagtgcggct ggtcggtggt acagcccggc gattccggct acgcaccttt
    17881 gattaatgaa ctcagcggcc agagcgcttc cgttccagtg tggtggtact tcacgcatgc
    17941 actgggcaaa ggcatcacca gcgccgccgt cgccgtattg ccctgcaagc cggacctgcg
    18001 ccagttgcgc tttgaggtgc agcacacccg gctcgccgat ccggcactgg cgcaggagct
    18061 acaggatttc gttaacgact gctacgggcc gtcgcgggca aggctcaagc ggctgagtag
    18121 cgatattagc gaggagcaaa gccgggaagt ggactgggtg ggatcgtctt tttacctcgc
    18181 cacgctaggc tattacgacc gcgatcactc ccgttcgccc cgcgccgcct ggccttatga
    18241 cgccagccgc gacgacggtc tgagcgatac gggtggcggt ggctatcctg cgtgtaagga
    18301 gtggtggtcc gatggcgata cggggctgaa aaaccgcctg ctgaagcagg tcgataccac
    18361 gctctggcag caactgcaaa aactgggaca gtctaaagag gagtatgaag aagcggtact
    18421 gcgctcgctg gtcagcccgc gcaatatgca ggtatcgcag ggcggactgg tgtacaacgg
    18481 gtttggtggg agtgcggggg ataacctcag ccagacgcag ttgctgagtc gtgtgggcgg
    18541 gatcatcggc gcggggcttt cgagctacgc cctgtttccg gcgtttgata acgtccgcca
    18601 ggcgctgccg atggtgcagg cgttcctcga aatggcgctg gtgatttgta ttccagtaat
    18661 tctgctgttc tcggcatggg atcttaaaac ggtcatcacg ctgagctttg tgcagttcgc
    18721 cctgttcttt ctcaccttct ggtgggaact ggcccgctgg ctggataact ggctgatgca
    18781 gatgatgtac gacagcgata cccactcata cttcaacctc tggggcctgc aaaacacctc
    18841 cgacgatctg attgtaaata tcattatggg cgtgatgttc ctggtgttac ccgcattctg
    18901 gctgggggcg ctgacgtggg cgggcatcag gatcggcgca gcagtcgctg gggggatggg
    18961 cagcgcggtc ggcgatatcc gcagcgctgg cgaacaggtc gggaagatga tcgtcagtaa
    19021 aacacgcatt ccctgaagat ttcgtactga cggtgtgatg aacagggctt gtagctgagt
    19081 ttatgcgcac cgcataaact gtcgtgtcag tgacaatctc gtggtaggta gctggagtct
    19141 gaaaaaacgc aacgggccgt taagcccgtt gttcgttaca tcaccgcagt tctatcactc
    19201 tcgcccgctc agcctgcttt cggtagtggt cgtatcgctc ccggaaccat gcttgctgtt
    19261 catcactcat attgccggta acggcatcga tatcaactgg ctggcccagg gcgtgcatac
    19321 gggcaaaact gcgcgccaga aaatcaaaat tctttaatgc gtttatatct tgtcggttca
    19381 ttagcatatc cctccagtca ttgtgtttac tgttcaaggt catatgcttt tctgatttca
    19441 gtatccgcct gaaaaagatc gttttttgag cgatgcgtgc gcatcaatct acctgtgttc
    19501 acatttcagg aacgacaaaa ctcttcagcc ccagtaatga ttttcccctc ccttttatca
    19561 cccgcgcaga aaacactacc gccagccatg cagtaagttc gccatgtatc cggaaggtgc
    19621 gaacggcgca gatgcgcagg atacccgtac cattccatcc cgcagggaaa cgccctgcga
    19681 agggtgttcc ctgctcattt acaggagaaa caccatgtcg cttttcgata aaaaaccgtc
    19741 gttaatgccg gtggttatcg cccctgatgt ctggcaatcc gcagtggaat ttcccgccga
    19801 cccgcagcag gtggaagaac ggctgggcaa tctgttgctc gtcgtactgc tgaccctgcg
    19861 caccgccggg acatcccgca agaaagtcac ctttacgatc tactgccagc cgccgcaggg
    19921 cgatatgaaa gtccctgtcg cattaccgct atcgctcaac tatcacggcc actatgtgca
    19981 ggtctcgatg gctgaacagg cataacctct ccccgccgga gtcgcttccg gatggaggag
    20041 gctccggctt ctttccatga ggaccacacg atggacagaa gcaacgatat ctaccagaaa
    20101 gtcaccgacc agattatcgc tgagctggaa aacggcaatg tgccgtggct ccgcccgtgg
    20161 cgcgatggcg agccgccgtt cccgatcaat gcgttatccg gtcgtccgta tcacggcatc
    20221 aatgtaccgc tgctgtggaa cagcgccgac aggcagagct tcgccagcga ccgctggctg
    20281 acgttccatc aggtcagcgc gctcggcggg caggtgcgcc ggggggagaa atcctctctg
    20341 gcggtgttgt atctgccgcg cacgcaggaa gagaccgacg cactcggcca gccgttgctg
    20401 gataaagacg gcgagccgaa ggtgcggcat ttcgggatta ttcgtgaatt ttggctgttt
    20461 aacctgtcgc agtgcgacgg cctgccgcag gcgctgtccg agccgtttac ccggcccgca
    20521 acgcctgtgg agatggctga agccattacg atgaatagcg gtgtgacgct gcgccatcgc
    20581 cgccagtcga aagcgtatta caaacccgcg accgatctgg tgatgatgcc gcacccgcaa
    20641 cagtttgaaa gcagcgaggc gtactacgcc acgctgctgc atgaactgac gcacgctacc
    20701 ggacacgctt cacgccttaa ccgccccggt attgccggag cggaacgcac cgaagcactc
    20761 tacgctgctg aagagttagt ggaggagatg ggtagcgcct tcctgtgcgc ccattgcggc
    20821 attcagggca ggctccagca cgccagctat atcggctact ggctggatat cctgcgccag
    20881 gacaagcggg cggtgatccg cgccagcggt atggcgcgcc acgccagtga atggctgctc
    20941 aacgctgtac cacaaccctg tcagcttagc gcctgacagc ccacccgcac ggggagcctt
    21001 ccccgccggg aagttgctcc cctttttctg aggatcaatg tatgggactc gatctcttct
    21061 ttttcgaacg gaaaaaaaac gcgcccgatg aaagccagta cgccagagtg ggttatttcc
    21121 gcaaggtgaa tccgctgctt aaatgggtgg ataaacacgt tgggctggtg cgcaaccaag
    21181 tgctgttaga agtcaccaaa gcaaatttgc aggcgcttct gagcgacctg cagagcctga
    21241 cgccggataa ctgcggcgcg gtttatccga cgattgacgg ctttttctat ggttataccg
    21301 attatggaga acactactgg aaagacgttc gcaccgtcac tctctgggtg aaggagacgc
    21361 tggagacgtt tgattttgag cgagacaggc tgatgtttta tgcctggtgg tagtattaaa
    21421 gtggccgaca aaacgtcggt cactttgcct cgtcggccag aaatacagcg ccagatatac
    21481 acatgagagc ataccgtttt tactaataaa aatgtaaaag gcccaaaggg agacactgca
    21541 aaggaagggc gacaagggca aaggggcctg cccggaaagg ggaaatatgc tgaagtggct
    21601 aaaaagacca tcccagagcc agccacctac gccagacgct acgctgccgg agggctggtt
    21661 tgcgccacaa tccgcagaac aattgctggc gacgccgtta cgccagtcct gtttgcacca
    21721 gttacggcaa ctgaccccgc tgccggatac cctgtttgat agctggatcc tcgcggcgat
    21781 tcatcgcttc gccgcgttgg tacaactgct tcccgcctca caaagtcatc accattccac
    21841 tcccggcggg ttgctggatc attcgctgga ggttgcctgc aacgccatcc gactgcgcca
    21901 gggttatctg ctaccaccgg aagcgggcgc ggaagaacag gcccggcagg ccagcgcatg
    21961 gacggtcgca gtggtctgcg tggcattact gcacgatacg ggaaaaatcg ccgtggatat
    22021 ggagattcat caacgtgatg gcacccgctg gcattcgtgg cagggttatc tggcacagcc
    22081 ttatcgctgg cagtaccaga gccacggtcg cgactatcat ctgcatccgg cggctaccgc
    22141 aatgctgatt aatcagttga taccctcgcc attactggac tggctggccg gatacccggc
    22201 ggcttttgcg tccctgattt accagctcac cggacactac gaacggtctg gcgcactggc
    22261 tgaactggtg cagcaggccg ataaagcctc tgtcgcccga tacctcggcg gcgatatcaa
    22321 aaccgcgctg gcgcaaaagc cctcttcctt cccccagcaa cttcttacag ccttgcggtc
    22381 gctggtacag aacgagtacc ggctcaataa tcccgaatgc ggatccgatg gctggctgac
    22441 ggaggatgcg ctgtggctta tcagcaaaac caccgccgac cgggttcgcg cctggctgct
    22501 ccagcatgga gtttccagcg tgccggaaaa caataaccgc ctgtttgacg aactgctgac
    22561 gcatcagctt attgttgcca acgaagataa agcgatttgg cgctgcatta tccgatcgga
    22621 taaaggctgg tcgcccgttg aaccgctgac gctactgcgc ctgacgccgt ctgtaatctg
    22681 ggggcaggat gatcaacgtc cggcctgttt cgatggtgat gtaacgccgg taattgaagc
    22741 cgaacccgct cctgaactta gcgcaccgac tcaaccgtta ccggagatag cagacgatcc
    22801 gaggcttggt gacaataaca atttcctgca gtggctgaag aaccacacta gtccttcagc
    22861 cacggtgaat acccggaacg gcaaggtaca tgtggtggaa aaccatcttt tcttgatttc
    22921 gccggggatc tttaaaacat acgcagcaga aaccacaggc gatagcggcg acggctggaa
    22981 actggcgcaa cgaatgtttc aggagtcagg gctggccctg cgctgtaatg atgacagttt
    23041 tatctggatc tgcgaggttc gggttccaca aaagaaaagt agtcagttga aagggtttct
    23101 gatggaagat ccgaagcagg tgttggggga gaaagtgccg gtgaataatc cgtggctgaa
    23161 gttgctgcgc gaatagctat ttatatcaaa atactctctt ccaccgagct acagcgaatg
    23221 ggctttaata cctaaagagc cgcatgatat tcaataattg atttcaattc ttcaggcgaa
    23281 aaaggcggat tacgccgatg caacatggcg tggcaatttg ggcagacggg aataaaatct
    23341 tttttgggat ccagagagta attctctttg atgtctgata gcggaatcag gtggtgtata
    23401 tggataaaac ctttcgctat ctcgccatac accttcatca tatcaatacc acatactttg
    23461 cacagacaac catgccaggc taatgcggcc tgtcgggcct tacggtcgcg ttcatagcga
    23521 ttaaccgtta cctgcgtagc tgcaccttca gtaaatacgc tctgcggtgg cagttcatca
    23581 ggaaaaatac tgatggcggg ctgctcgtta cccaaatgtt tctcaacgat ctggaaccct
    23641 tgatcggtaa gcaacttgac gcatttcgac ttgataccac cactaaaatc ttcaggggaa
    23701 aactcaacgc cagtcatatt tgaagcagca ataccgacta tagctttcgg tggataactg
    23761 cgcccttcaa agaccacatc atacagccgg gactcgctga actggtggcg tacaccttca
    23821 tcataggcac gcatagccgc aataatgcat tccctggaaa gctggtctgg cagtttcttc
    23881 ttcatttaca actcatcatc cctgaaacga catttgctaa ctctttattt tatagcatat
    23941 taatttatcc ggtgataaac agcactcttc aatttcgcgt taccgggata ggggcatcag
    24001 tgcagcggca acgtaacgta gcggcagaga aatcgcgcct cattctggtt gtccagacgt
    24061 acctgaaaga tatcgtgtgt atcctgcgcg gatgcatcct cacgccacgc cagcagaccc
    24121 cgttggttca tgcggataat aatctgatcg ggcgtttcgc cctgattcaa tgcttcgact
    24181 acctgttgta ggggaagcgg tagcgtctga gcaggccaca tcacttcaac acttacgagc
    24241 tggcggtgtg tccgaggtga catcggtggt ttcctccatg gctggaattt agcggttaat
    24301 tagccatgaa ataggggcat tttttactgt ttcaactact gccgagatgg ctacggaata
    24361 cgcgctgact gatgcaaaaa tagcttctct tatgtgctct ggaaatccag atggtacgct
    24421 agtgaaaatg gtggttccgt ccggatagcg ccatggcctt tttgttcata tttccacaga
    24481 tcaccactcc tgctcccagc catcagaaga gtttttttac ccctccagct tacgacctga
    24541 agcttaaggt ccagagcggc aagcagctta aagagcgtat ccaggctggt cccctcagga
    24601 tggttctcaa actctgaaac cgttgactgt ttcatgccca tacttgctgc agtttcacgc
    24661 tgggtcaggt tacgttgctt gcgtgcatcc ctgattgcct gagacaacat tttgggtgag
    24721 gttacgttca ttttatcccc caggatagat attttgagca tatcccctat aggggataaa
    24781 aattcatatt ctcagcggag aaaagattaa taactaaatg aattaaaatt acttttcatg
    24841 gaaattaagg ggggacgata ttcctcgagc cagtaaatat gaacattgcc agatggcaga
    24901 acgcgccatt ttacaagcat tttccataac agatataaga ctgattgagc ggttggatta
    24961 agcatgacga tatgtttaaa aaaaccagtt ttatcgacat aacgccaaaa tatgtttaca
    25021 gcatcgacat atcaacgcca tatgtacatt ttttggcatt ttttaaacat atcatcagca
    25081 ggctttattt tttgctatcg aaatctacag tccctcgttt caatacaaaa attccagcgt
    25141 gtcagctacc ttttctgggt ttctttattg gttttagcag tgcagcaaga gatgcagatg
    25201 tcacgacttc tgttttaact tcagaatcga ttgatttttc ttgtgatgtc gagaattgct
    25261 tgtcgaaggc atcgtttttt atcacgtttc cggtatgcct gtttgtgtga gagatagcag
    25321 caggacgaga aacacgcgtt ttatttatta gccgggaatc ctgaagttgg cgtccctgtt
    25381 catcctcagc ggcaagtttg ttcagatcct tttcaagacc caaaatctgc ataactgaca
    25441 aataagcgcc gattgtcacg cccgtcccgc ctgattcaag agaacgcaag gtcattggcg
    25501 acatcccggc tcgctcagca acccgcttcg ctgtgagctt acgtcgcagt cgcgccagct
    25561 taagccgttc gccaaactca gcaagcaact gattagtacc gggaactaag ggagccgt
//
LOCUS       DAZGAP010000069        25223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGAP010000069 DAZGAP010000000
VERSION     DAZGAP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.069"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(90..173)
                     /gene="yohP"
                     /locus_tag="ACHLAM_002330"
     CDS             complement(90..173)
                     /gene="yohP"
                     /locus_tag="ACHLAM_002330"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO7853848.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            397..1833
                     /gene="mdtQ"
                     /locus_tag="ACHLAM_002331"
     CDS             397..1833
                     /gene="mdtQ"
                     /locus_tag="ACHLAM_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO7853849.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPVMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1886..2647
                     /gene="yohF"
                     /locus_tag="ACHLAM_002332"
     CDS             1886..2647
                     /gene="yohF"
                     /locus_tag="ACHLAM_002332"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7853850.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVRPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2766..3344)
                     /gene="yohD"
                     /locus_tag="ACHLAM_002333"
     CDS             complement(2766..3344)
                     /gene="yohD"
                     /locus_tag="ACHLAM_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7853851.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILAVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3514..4101
                     /gene="yohC"
                     /locus_tag="ACHLAM_002334"
     CDS             3514..4101
                     /gene="yohC"
                     /locus_tag="ACHLAM_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO7853852.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4275..5207
                     /gene="pbpG"
                     /locus_tag="ACHLAM_002335"
     CDS             4275..5207
                     /gene="pbpG"
                     /locus_tag="ACHLAM_002335"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO7853853.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5246..6961)
                     /gene="dld"
                     /locus_tag="ACHLAM_002336"
     CDS             complement(5246..6961)
                     /gene="dld"
                     /locus_tag="ACHLAM_002336"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7853854.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGVGKTSKRKN
                     WQEVE"
     gene            7157..9454
                     /gene="bglX"
                     /locus_tag="ACHLAM_002337"
     CDS             7157..9454
                     /gene="bglX"
                     /locus_tag="ACHLAM_002337"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO7853855.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATAKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9603..10520
                     /gene="osmF"
                     /locus_tag="ACHLAM_002338"
     CDS             9603..10520
                     /gene="osmF"
                     /locus_tag="ACHLAM_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO7853856.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10527..11684
                     /gene="yehY"
                     /locus_tag="ACHLAM_002339"
     CDS             10527..11684
                     /gene="yehY"
                     /locus_tag="ACHLAM_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO7853857.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACVIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHLLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGISGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11677..12603
                     /gene="yehX"
                     /locus_tag="ACHLAM_002340"
     CDS             11677..12603
                     /gene="yehX"
                     /locus_tag="ACHLAM_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO7853858.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRRKERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLVEA"
     gene            12608..13339
                     /gene="yehW"
                     /locus_tag="ACHLAM_002341"
     CDS             12608..13339
                     /gene="yehW"
                     /locus_tag="ACHLAM_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO7853859.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKSMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13320..13427)
                     /gene="yohO"
                     /locus_tag="ACHLAM_002342"
     CDS             complement(13320..13427)
                     /gene="yohO"
                     /locus_tag="ACHLAM_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO7853860.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13487..14218)
                     /gene="mlrA"
                     /locus_tag="ACHLAM_002343"
     CDS             complement(13487..14218)
                     /gene="mlrA"
                     /locus_tag="ACHLAM_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO7853861.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14440..16125
                     /gene="btsS"
                     /locus_tag="ACHLAM_002344"
     CDS             14440..16125
                     /gene="btsS"
                     /locus_tag="ACHLAM_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO7853862.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            16122..16841
                     /gene="btsR"
                     /locus_tag="ACHLAM_002345"
                     /gene_synonym="yehT"
     CDS             16122..16841
                     /gene="btsR"
                     /locus_tag="ACHLAM_002345"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO7853863.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16888..17358
                     /gene="yehS"
                     /locus_tag="ACHLAM_002346"
     CDS             16888..17358
                     /gene="yehS"
                     /locus_tag="ACHLAM_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO7853864.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17400..17861)
                     /gene="yehR"
                     /locus_tag="ACHLAM_002347"
     CDS             complement(17400..17861)
                     /gene="yehR"
                     /locus_tag="ACHLAM_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO7853865.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(17986..19989)
                     /gene="yehQ"
                     /locus_tag="ACHLAM_002348"
     CDS             complement(17986..19989)
                     /gene="yehQ"
                     /locus_tag="ACHLAM_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO7853866.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGKCLCQLAALTSRLNSAQEIARRDSVGEVPPMPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEHSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(19986..21122)
                     /gene="yehP"
                     /locus_tag="ACHLAM_002349"
     CDS             complement(19986..21122)
                     /gene="yehP"
                     /locus_tag="ACHLAM_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7853867.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(21115..23394)
                     /gene="yehM"
                     /locus_tag="ACHLAM_002350"
     CDS             complement(21115..23394)
                     /gene="yehM"
                     /locus_tag="ACHLAM_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO7853868.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPYAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREDFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLVILEIPGVVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFNQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLVQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23405..24493)
                     /gene="yehL"
                     /locus_tag="ACHLAM_002351"
     CDS             complement(23405..24493)
                     /gene="yehL"
                     /locus_tag="ACHLAM_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO7853869.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5895 a         6403 c         6866 g         6059 t
ORIGIN      
        1 tatatgaagc aatgcacatg acaggagcgc aacaggatga gagactgaaa tattctcgtt
       61 attttcggat ctgatgacat taattaattt taaaatatca tcttaaatac gccagtcacc
      121 accagtagcc caatcaggaa aataatcaat acagcccaga gtataatttt cattggcgtt
      181 cctttatttg tgagtgctga ataagtatag gaaaaaacga agccctttgc cgctatccat
      241 aaacgattaa gataaatcgg aagatatcat tcacttggtg tatatttttc tggatatgtc
      301 gttttattgc cactgatgta ttgccacgcg aaactaaaaa atatatcgca acacacggta
      361 atctccgcgc accttttctt acctgattat tcccccatga atcgtgattc cttttatcca
      421 gccatcgcct gttttccgct gttactgatg ctggccgggt gtgcgcctat gcatgaaacc
      481 cgccaggcgt taagccagca cacgcccgct gtgcaagttg acaccgcatt acccacggcg
      541 ctgaaaaatg gttggccaga cagccaatgg tggctggagt atcacgataa tcaactcacc
      601 tcattaatta ataacgcact acagaacgca ccggttatgc aggtggctga acagcgcatt
      661 cagctggctg aagcccaggc gaaggcaatt gctactcagg atggtccgca gctcgacttt
      721 tctgcggata tggagcggca aaaaatgtcg gcagaaggct taatggggcc gtttgctctg
      781 aacgatccgg ccgcaggtac gaccggtccg tggtacacca acggtacttt tggcttaacg
      841 gcgggctggc atctcgatat ctggggaaag aatcgggcgg aggtaactgc tcgcctgggt
      901 acggttaaag cacgagcggc ggaacgcgag caaacccgcc aattgctggc tggcagcgta
      961 gcccgcctgt actgggagtg gcaaacccag gcggcgttaa acacggtctt gcagcaaatc
     1021 gaaaaagagc agaacaccat tatcgccacc gatcgccagc tatatcagaa cgggattact
     1081 tcttcagttg aaggtgtgga aaccgatatt aatgccagca aaacccggca gcagctcaac
     1141 gatgtcgcgg ggaaaatgaa aattattgag gcacggttaa gtgcacttac aaatcaccag
     1201 acaaaggcat tgaagcttaa tccggtcgcg ttgccgaaag tggcaagcca gcttcctgat
     1261 gaactggggt actccttact ggcccggcgg gcagatttgc aggcggcgca ctggtacgtt
     1321 gagtcatcgt taagcactat tgatgcggca aaagcggcat tttatcctga catcaacctg
     1381 atggccttcc tgcaacagga tgcgttgcac ttaagcgatc tgttccgtca ttccgcgcag
     1441 caaatgggcg ttacggcagg cctgacgcta cccattttcg atagtggtcg tcttaacgcc
     1501 aacctcgata tcgcaaaagc cgaaagcaac ttgtctatcg ccagctacaa caaagcggtg
     1561 gttgacgcgg tgaatgacgt ggcgcgggca gccagtcagg ttcagacact ggcggagaaa
     1621 aaccagcatc aggcgcaaat tgagcgcgat gccttgcgtg tagtaggtct tgcacaggcg
     1681 cgctttaacg cgggcatcat tgccggttcc cgcgtcagcg aagccagaat cccggcgctt
     1741 cgcgagcggg ccaatggcct gttattgcaa gggcagtggc tggatgcctc cattcaactc
     1801 actggtgcgt tgggcggggg ttacaaacgc tgatgatatg cgccttctat acttaacgtt
     1861 tattcagcgt taagtggaga actcgatggc acaggttgcg attattaccg cctccgattc
     1921 ggggatcggc aaagagtgcg cgttattact ggcgcagcag gggtttgata ttggcattac
     1981 ctggcactct gatgaagaag gggcaaaaga tactgcgcgt gaggtggtta gccacggcgc
     2041 acgtgcggag atcgtgcagc tggatctcgg taaactgcca gaaggtgcgc aggcgctgga
     2101 gaaactcatt caacgactgg ggcgcatcga tgtgctggtg aataatgcgg gtgcaatgac
     2161 caaagcgccg tttcttgata tggcttttga tgagtggcgc aagattttta ccgttgatgt
     2221 cgacggtgca ttcttatgct cgcaaattgc ggctcgtcag atggtgaaac aagggcaggg
     2281 cggtcgcatc atcaacatta cgtcggtgca tgaacatacg ccgctgccgg atgccagcgc
     2341 ttacacagca gctaaacatg cgctcggcgg gttaaccaaa gcaatggcgc tggagttggt
     2401 caggcataaa attttggtga acgcagtcgc acctggggcg atcgccacgc caatgaacgg
     2461 catggatgat agcgatgtga ggcccgacgc ggagccttcg attcccttgc gacgttttgg
     2521 cacaacgcat gagattgcca gcctggtggt atggctttgt tcggagggcg caaattacac
     2581 caccgggcag tcgttgatag tggatggcgg ctttatgttg gcgaatccgc agttcaaccc
     2641 agaatagcgc agtgaggtgc gtggttattt caatgggtta ggccagataa gacacgccag
     2701 cgtcgcatca ggcatggaac atccggtgcc ggatgtggcg tgaatgctta tctgtggcaa
     2761 ctgctttacg cctgattatc cggctttttc ttcccgcgtc gtttcagcca ccagcgcacg
     2821 cctaccacca gaaccacggc cagaatcaac cagacccagt gcttcaaatg ctggtcgaga
     2881 ttgtgcaacc acggcgcaat cacctgacca ccagcataac cgatagtggt aaaaatcaac
     2941 gcccaggcaa atgcgccgag aatattcagc ggcagaaaga ttttcggcgg cagctggctg
     3001 gcaccaatca gcgtcgggcc aatcacccta aagccataca taaaacgcgt accaatgaca
     3061 aacagatacg gatggcgttg gataagtttc tgcgcccgct caattttatc ctgatgtttc
     3121 gagaaacggc gtaatagctt gccgccaaag cgccgcccgc acagataaag cacctggtcg
     3181 ccaatcatgc cgccaagcgc cacagaaagt accaccagcg ggaactttaa tagcccctga
     3241 tgtgccgcaa cgcctcccag caaagtcacg gtttcacctt ccgccaggct accgatcacc
     3301 agcgcggcat aaccatattg tgagatgagt gtattgagat ccataggcca gtctgtctcc
     3361 ctttgcttgc ctgtaagcat acaccctgtg aatagttcac gctgaaaaag gacgtacatc
     3421 agacggtgca aattacatga gcgaacaaaa aataatcaca agctgactta tacttgaact
     3481 gtcaggttca tgaactcaca acaagggggc ggtatgagcc atgtctgggg actgttttcc
     3541 catcccgatc gtgaaatgca ggtgattaat cgcgaaaacg aaacgatttc tcaccattac
     3601 acccaccacg ttttgctgat ggcggcgatt ccggtgattt gcgccttcat tggcactaca
     3661 cagattggct ggaattttgg cgatggcact atcctgaagt tatcctggtt taccggactg
     3721 gcgctggctg tcttgtttta cggcgttatg ctggctgggg ttgcggtgat ggggcgggtc
     3781 atctggtgga tggcgcgtaa ttatccgcag cgtccgtcac ttgcgcactg catggtcttc
     3841 gcgggctacg ttgcaactcc gctgttttta agcggtctgg tggcgcttta cccgctggtc
     3901 tggttgtgtg cgctggtcgg cacggtggcg ctgttttaca ccggctacct gctgtatctc
     3961 ggtattccga gcttcctgaa tatcaacaaa gaggaaggac ttagcttctc cagttcgaca
     4021 ctggcgatag gcgtgctggt gctggaagtg ctgctcgccc ttaccgttat actatggggc
     4081 tacggttatc gtttgttcta gaaagttctt ttgcgctgca ttgctggcgt aaatgtcagc
     4141 aatgcagcat ttcctcacga ttcttctttg acgatcgcac tttttgctcg ttatgatggc
     4201 gtcgctagcc tcagtaaatc cttactccgg cggtgcgcaa cccgtgcgcg tgaaccacta
     4261 tctgaatgct catcatgccg aaatttcgag tttctttatt tagcctggcc ctgatgctgg
     4321 ctgtgccttt ggcaccgcag gccgttgcga aaacggcagc cgctaccacc gcttcacaac
     4381 cggaaattgc ctccggtagc gcgatgattg ttgatctgaa taccaacaaa gtgatctatt
     4441 cgaaccaccc ggatctggtg cgtccgattg cgtctatcag caaattaatg accgcgatgg
     4501 ttgtgctgga tgcacgactg ccgctggatg aaaaactaaa agtggatatc agccagacgc
     4561 cggagatgaa aggggtctat tcgcgcgtac gactgaacag cgaaatcagc cgtaaagata
     4621 tgctgttgct ggcgctgatg tcttcagaaa accgcgcggc ggcaagcctt gcgcaccatt
     4681 atcccggtgg ttacaaagcc tttattaagg cgatgaatgc gaaagcgaaa tcgctcggaa
     4741 tgaacaacac gcgctttgtt gaaccgaccg gattgtcggt gcataacgtt tcaactgccc
     4801 gtgacttaac caaactgctc attgccagca aacaatatcc gttgattggt cagttaagta
     4861 ctaccaggga agatatggca accttctcta acccgacgta tacgctgccg ttccgcaata
     4921 ctaaccatct ggtgtatcgc gataactgga atattcagtt aaccaaaacg ggctttacca
     4981 atgcggcggg ccattgtctg gtgatgcgta cggttatcaa taataaaccg gtggcgctgg
     5041 tagtgatgga cgcgtttggc aaatataccc attttgccga tgccagccgc ctgcgtacct
     5101 ggattgagac cggtaaagtg atgcctgttc cggcagcagc gttgagctat aaaaaacaaa
     5161 aagccgccca aatggcggcg gcggggcaga cggcacagaa cgattaaatg aatgacggat
     5221 ggcgatactc tgccatccgt aatttttact ccacttcctg ccagtttttc cgcttactgg
     5281 ttttaccaac ccccggattc atactgttgg tcggatcgtt ctcgcgataa aacttctgca
     5341 acgtttccgg tgctttatac aaatgaccga cgttatgctc ggcagggtat tgcgcgccgc
     5401 gctgctgtag cagttccagc atctgttctt tcaacgcatg cacatccacg ccttttttaa
     5461 cgatgtaatc ctgatggaag acatagcaca taaaatggcc gtaatagagc ttgtgcacca
     5521 gctggctgtc gatctccggc ggtaaatgct cataccactc ggtgtcgtta cgccgcagag
     5581 cgatatccag tgccagaatg tcttcgactt catcggaatg caccgcctga taacgaattg
     5641 ctgcgcccgc agcggcgaaa cggtgtaaaa acgctttgct gccttcctcc ggcgtacaga
     5701 caaagaaatc gccttcggcc tgtttgaaat aatccaccag ccacgatttg gcttcaccga
     5761 cgccatcgcc cgccattttt aacagcagat gatgttcgta tttatcgcgc cagtttttca
     5821 tgcgcggcgg taaatggctg gggaacaggt gaccgaattt ttgcatcgca cggtcggtaa
     5881 aatgcggacg gaagaatttc actttctcca gcatcgcatc ggtgcgcccc ttgagattaa
     5941 agaagaacgg catcttgtcg gtgcctaact tatcaatcat caggaaggtg tctttgccgt
     6001 atttttccgc tatatcgtag atatcccggt gcatatattc cccggcaacc ggcagatttt
     6061 cgaaattagc cagaatatga cggcggattt cggtcagtac ttccggctgg ttggtgccga
     6121 tataaaacac ctgctgattt ttttccgctt cgaaggtatc aagacgtacc gcaaagacgg
     6181 ccagcttccc ggcgcaacca gaagattcaa ataaccgatc cggatcggcg ttataacgtg
     6241 cgggcgtgtc ggcttcaata tcacgaacgc ggtggacata gtcataatcg tgggcgtgac
     6301 gaccatcgtg gcgcacatcg tcatctttga tgcgatcgtc atccagcttg ctaaggatct
     6361 gttccggcgt ttcgcccaga tcaatcccca gatggttaac cagcgtcagc ttgccatctt
     6421 catttatacg cgcgaataac gacatttcgg tatacgccgg gccacgttgc accagcgagc
     6481 cgccggagtt gttacaaata ccgccgatga ccgatgcgcc tatacacgat gatccaatca
     6541 ccgagtgcgg ttcgcgtccc agcggtttga gggctttttc cagcgaataa agcgtggtgc
     6601 ccggataggc cagcacctgt tcgcccttgc cgagaacgtg cagcttgtcg agacgcaggg
     6661 tgctgatgat cacgatatcg cgatcatagt cgttaccgtt tggcgtcgat ccttcggtca
     6721 ggcctgtatt ggcagcctgc atcagaataa ttttgtcggc ggtgacgcag gctttcagca
     6781 cccgccacaa ttccagtagt gagccaggga aaacgacagc cagtgcgtcg ccctgaccag
     6841 aacggaagcc cttgcgatag cgggccgttt ttgcgggatc ggtgagcagg tgtgaatgac
     6901 cgaccagacg ggcaagttca ttcaaaaagg ctttattatc agttgttgtc atggaagaca
     6961 ttttccactc cttgtggtgg cgaaaaaata tcacaaacta gaatagcgcc ttgtatgaca
     7021 attcagcgag tgaatatttt gaaaattcag acaataaact gagcattctt tttctctctc
     7081 catcatgctt atggcaaact acggtttttg caccattttc gctgccacgt cgggcaacaa
     7141 aggaagaaaa atccatatga aatggctatg ttcagtagga atcgcggtaa gtctggccct
     7201 gcagccagca ctggcggatg atttattcgg taaccatccc ttaacgcctg aagcgcggga
     7261 tgcgttcgtc accgaactgc ttaagaaaat gacggttgat gagaaaattg gtcagctgcg
     7321 cttaatcagc gtcggcccgg ataatccgaa agaggcgatc cgcgagatga tcaaagacgg
     7381 gcaggttggg gcgattttca acaccgtaac ccgtcaggat atccgcgcca tgcaggatca
     7441 ggtgatggaa ttaagccgcc tgaaaattcc tcttttcttt gcttacgacg tgctgcacgg
     7501 tcagcgcacg gtgttcccga ttagcctcgg tctggcctcg tcttttaacc tcgatgcggt
     7561 gaaaacggtc ggacgtgtct ctgcttatga agcggcagat gatggcctga atatgacctg
     7621 ggcaccgatg gtcgatgtct cgcgcgatcc gcgctgggga cgtgcttccg aaggttttgg
     7681 cgaagatacg tatctcactt caacaatggg taaaaccatg gtggaagcga tgcagggtaa
     7741 aagcccggca gatcgttact cggtgatgac cagcgtcaaa cactttgccg catacggcgc
     7801 ggtagaaggc ggtaaagagt acaacaccgt cgatatgagt ccgcagcgcc tgtttaatga
     7861 ttatatgccg ccgtacaaag cggggctgga cgcaggcagc ggcgcggtga tggtggcgct
     7921 gaactcgctg aacggtacgc cagccacctc cgactcctgg ctgctgaaag atgttctgcg
     7981 cgaccagtgg ggctttaaag gcatcaccgt ttccgatcac ggcgcaatca aagagctgat
     8041 taaacatggc acggcggcag acccggaaga tgcggtgcgc gtggcgctga aatccggcat
     8101 caacatgagt atgagcgacg agtattactc gaagtatctg cctgggttga tcaaatccgg
     8161 caaagtgacg atggaagagc tggatgacgc tgcccgtcat gtactgaacg ttaaatatga
     8221 tatggggttg tttaacgacc catacagcca tttggggccg aaagagtctg acccggtgga
     8281 taccaatgcc gaaagccgcc tgcaccgcaa agaagcgcgt gaagtggcac gcgaaagcct
     8341 ggtgttgctg aaaaaccgtc tcgaaacgtt accgctgaaa aaatcagcca ccattgcggt
     8401 ggttggcccg ctggcagaca gcaagcgtga cgtgatggga agctggtccg cagccggtgt
     8461 cgccgatcaa tctgttactg tgctaacagg gattaaaaac gccgtcggtg aaaacggtaa
     8521 agtgctgtat gccaaagggg cgaacgttac cagtgacaaa ggcattatcg atttcctgaa
     8581 tcagtatgaa gaagcggtca aagtcgatcc gcgttcgccg caagagatga ttgatgaagc
     8641 ggtgcagacc gcgaaacaat ctgatgtggt ggtggctgta gtcggtgaag cacaggggat
     8701 ggcgcacgag gcttccagcc gtaccgatat cactattccg caaagccaac gtgacttgat
     8761 tgcggcgctg aaagccaccg ctaaaccgct ggtgctggtg ctgatgaacg ggcgtccgct
     8821 ggcgctggtg aaagaagatc agcaggcgga tgcgattctg gaaacctggt ttgcggggac
     8881 tgaaggcggt aatgcaattg ccgatgtatt gtttggtgat tacaacccgt ccggcaagct
     8941 gccgatgtcc ttcccgcgtt ctgtcgggca gatcccggtg tactacagcc atctgaatac
     9001 cggtcgcccg tataacgccg ataagccgaa caaatacacg tcgcgttatt ttgatgaagc
     9061 taacggggcg ctgtatccgt tcggctatgg tctgagctac accactttca ccgtctctga
     9121 tgtgaaactt tccgcgccga ccatgaagcg tgacggcaaa gtgaccgcca gtgtgcaggt
     9181 gacgaacacc ggtaagcgcg aaggggcgac ggtagtgcag atgtacttgc aggatgtgac
     9241 ggcttccatg agtcgtccgg tgaagcagct gaaaggcttt gagaaaatca ccctgaaacc
     9301 aggcgaaacc cagaccgtca gcttcccgat tgatatcgag gcgctgaagt tctggaatca
     9361 acagatgaaa tatgacgccg agcctggcaa gttcaatgtc tttatcggca ctgattccgc
     9421 acgcgttaag aaaggcgagt ttgagttgct gtaatgcttt agtaagggcg cgacgtctgc
     9481 gccctttaag tatttatctg attctgcaac tgaatccgcc cagacaggct aaccctgtca
     9541 ttatcaacta tgcttttctc ttaattcgct gaaaacaaca gtaaaagaat gaggaaagca
     9601 tcatgccact ctcaaagctc tgggcaggtt cattggtttt gttggcagcc gtgagcctgc
     9661 cgctacaggc ggcttccccc gttaaggtcg gttcaaaaat cgataccgaa ggcgcgctgc
     9721 tcggcaatat cattttgcag gtgctggaaa gtcacggtgt tcctacggta aataaagtgc
     9781 aactgggaac gactcccgtg gtgcgcgggg cgattacttc cggtgaactg gatatctatc
     9841 cggaatatac cggcaatggc gcgtttttct ttaaagatga aaacgatgcg gcatggaaaa
     9901 atgccgggca aggctacgag aaagtcaaaa aactcgatgc agagcaaaac aagttaatct
     9961 ggctgacgcc cgcgcctgca aataacacct ggactatcgc cgtgcgtcag gatgtggcag
    10021 agaaaaacaa actcacttcg cttgccgacc tgagtcgtta tctgaaagag ggcggcacct
    10081 tcaaactggc ggcctcggca gaatttattg aacgcgccga tgccttaccc gcttttgaaa
    10141 aagcctacga ctttaaactc gatcaggatc agttgctgtc actggctggt ggcgacacgg
    10201 cggtaacgat caaagccgct gcccagcaaa cttctggcgt taatgccgca atggcttacg
    10261 gcactgacgg tccggtcgca gcgctggggt tgcaaacctt aagcgatccg caaggcgtgc
    10321 aacctatcta cgcgcctgca ccagtggttc gtgaatcggt gttgaaggag tatccgcaaa
    10381 tggcacagtg gctacagcca gtcttcgcca gcctcgatgc aaaaacattg cagcaactga
    10441 atgccagcat tgcagttgaa ggactggatg ccaaaaaagt ggctgccgac tacctgaaac
    10501 aaaaagggtg gacgaagtaa tttcccgtga cttatctacg tattaatcct gttctggcgc
    10561 tgctgctgtt gctgacggca atcgcagcgg cgctgccgtt tatcagttac gcgcctaatc
    10621 gtttagtttc gggtgagggg cgtcatctct ggcagctgtg gccgcaaacg atctggatgc
    10681 tggtgggcgt tggttgcgcc tggctgaccg cctgtgttat tcccggtaaa aaaggcagca
    10741 tttgtgcact cattctggcg caattcgtct tcgtattgct ggtgtgggga gctggaaagg
    10801 cggcgacaca actggcacaa aatggcagtg cgctggcgcg taccagcctc ggcagtggtt
    10861 tctggctggc tgcggcgctg gcattgctgg cctgtagcga tgccatccgc cgaatctcca
    10921 cgcatctgct gtggcgctgg ttgttgcata tgcagattgc cattattccg ctgtggttgc
    10981 tgtactccgg cacgcttaac gatctctcgc taatgaaaga atacgccaac cgtcaggatg
    11041 tgtttgacga cgcgctggca caacatctga cgttgctgtt tggtgcggtg ctgcctgcgt
    11101 tagtgattgg tgtgccgttg ggcgtctggt gctacttttc cactgcgcgg cagggagcga
    11161 ttttttcgct actcaatgtc attcagaccg tgccttcggt ggcgctcttt ggcctgttga
    11221 ttgcgccgct tgccgcgctg gttacggcct ttccgtggct gggtaagctc ggcatatcag
    11281 gaaccggaat gacacccgca ctgattgcgc tggtgctcta tgccttgctg ccgctggtgc
    11341 gcggcgtggt agtcggcttg aaccagatcc cgcgcgatgt gctggagagc gccagagcga
    11401 tgggcatgag cggggcgcag cgattcctgc atgttcagtt accgctggcg ttaccggtat
    11461 ttctgcgtag cctgcgggtg gtgatggtgc aaaccgtcgg catggcggtg attgcggcgt
    11521 taatcggcgc aggcggtttt ggtgcgctgg ttttccaggg gctgctaagc agtgccattg
    11581 atttagtgtt gctgggggtg atcccggtaa ttgttctggc ggtgctgatc gatgcgctgt
    11641 tcgatttgct tatcgcactg ctgaaggtga aacgtaatga ttgaatttag ccatgtcagc
    11701 aaactgttcg gcgcacaaaa agccgttaac gatctcaatc tcaattttca ggaggggagt
    11761 ttttcggtgc tgattggtac atcaggctcc ggtaaatcca ccaccctgaa aatgattaac
    11821 cgcctggtgg agcatgacag cggcgtgatc cgctttgccg gagaagaaat tcgctcgctg
    11881 ccagtgctgg agttgcgccg ccggatgggc tatgccattc aatctattgg cctgtttccc
    11941 cactggagcg tggcgcaaaa tattgccacc gtgccgcaat tacaaaaatg gtcacgggcg
    12001 cggatcgacg atcgtatcga cgaattaatg gcgctactgg ggctggagcc aaatttgcgt
    12061 gagcgttatc cgcatcagct ttccggtggt cagcagcaac gtgtgggagt ggcgcgcgca
    12121 ctggctgccg atccgcaagt cttactgatg gatgaacctt ttggcgcgct ggacccggta
    12181 acgcgcggcg cgttgcaaca agagatgacg cgcatacacc gtttgctggg gcgtaccatt
    12241 gtgctggtca ctcatgatat tgatgaggcg ctacgactgg cagaacatct ggtgttgatg
    12301 gatcacggtg aagtggtgca gcagggcaat ccgctgacga tgctgactcg tccggcgaat
    12361 gattttgtcc gccagttttt tgggcatagt gaactgggtg tccgcctgct ttcgttacgt
    12421 agcgtggcgg attacgtgcg tcgcaaagaa cgggcagaag gtgaggcgct ggcagaagag
    12481 atgacgctac gcgatgcgct ctccctgttt gtcgcgcggg gatgtgaagt gctgccggtg
    12541 gtgaacacgc agggcgagcc ttgcggcacg ctgcattttc aggatctgct ggtggaggcg
    12601 taagcgtatg aagatgttgc gcgatccgct gttctggctc attgctttgt ttgtggcact
    12661 gattttctgg ctgccttaca gccagccgct gtttgctgcc ttgttcccac aactgccacg
    12721 ccccgtttat cagcaagaaa gttttgctgc tctggcactg gctcatttct ggctggtggg
    12781 aatttcgagt ttgtttgcgg tgatcattgg cactggtgca ggaattattg ttactcgccc
    12841 gtggggcgcg gaatttcgcc cactggtgga aactattgcc gccgttggac agacttttcc
    12901 gcccgtcgcg gtgctggcga ttgccgttcc ggtgattggg tttggtctgc aacccgcgat
    12961 tatcgccttg atcctttatg gtgtgctgcc cgtcctgcag gcgacacttg ccgggctggg
    13021 agcgattgat gccagcgtga cagaagttgc gaaaagtatg ggaatgagtc gaggtcagcg
    13081 actgcgtaag gtcgagctac cgctggcggc tccggtgatt ctggcgggtg tgcgaacttc
    13141 ggtgattatc aacattggta cggcgacgat cgcctcaacg gtaggggcca gcacgctggg
    13201 tacgcccatc atcatcgggc ttagcggatt taataccgcg tatgtgatcc agggggcgtt
    13261 actggtggca ctggcggcga tcattgcaga ccgcctgttt gaaaggctgg tgcaggcgct
    13321 tagccagcac gcaaaataaa ggtataacct gcgagcatga cgccaccaat tccgcctaac
    13381 gccataaaca ggaacagggc gatgacccca attttagcta tgcgcataat gcactcctta
    13441 tgttaacgaa aggattgtac agtaaagcgc atttgttaac gaatcattaa atgccgagtg
    13501 ggaaaatatc atggccttgt tcctgccaac tggtgagttg ctgctgttgg gcggaggttc
    13561 gattttcacc gcaccacacc agcaatgtac ggccttcgaa cagttcaggg cgtagttgat
    13621 tgagcgagtg ggcgaggaca tcaatgcgcc atccttgttg actggcaatc cagccctcca
    13681 gccacagacg ggtggtatcg tgaatattcc agccaaccac cagcgcatct ttaccctgtt
    13741 ttttacgtgc cgaagccagg caaatggcga tgtagttgat cagtacgccg tcgaggatcg
    13801 ccagcagcgc ctggagagtc ggttgttggc actgaagtcg tcggcgcaga ggaataaaca
    13861 gatgcgtggt gagtgtctgg gcggggtaat cctgaccgcg ctctttgatc cacgttcgca
    13921 ggctatgcag attgccgctt tgcaggtaag tcagtaatgt ttcttgctga tcgcgccagc
    13981 cgttctgcac atcaacattt tcattactga gcagcatttt aactttgctg acctgcacgc
    14041 cgttgtcgat ccagcgtttg atctcgcgga tccggtcaat atcggcatca ttgaacagcc
    14101 gatgaccgcc gtctgtccgt tgcggtttca gcaatccgta acgcctctgc cacgcgcgta
    14161 acgtgacagg gttaatatca caaagcaacg ccacttcacc aattgtgtaa agcgccatcg
    14221 tctcaccctt gctcgcgagg tcccggttta actttagacg cagttttgcg aaccaggtag
    14281 ttttgcccgt tttttgtgca tctatagggt gattttattt ttgccaggcg attttgagtg
    14341 atcgtactca cgaattctca tttttctgca agagttcaaa gaaagttaaa cgcaggcaat
    14401 gtatgttacg cgttttaaag ggaagtgtgg tttgcgggta tgtacgattt taatctggtg
    14461 ttgctgctgc ttcagcagat gtgcgttttt ttagtcattg cgtggttaat gagtaaaacg
    14521 ccattattca taccgttaat gcaggtcacg gttcgtctgc cgcataaatt tctctgctac
    14581 atcgtctttt ccatcttctg catcatgggc acctggtttg ggctgcatat tgacgattct
    14641 attgccaata cccgtgcgat aggcgcggta atgggcggct tactcggcgg tccggtcgtc
    14701 ggtgggctgg ttggtctgac cggcgggtta catcgatatt cgatgggggg catgaccgcg
    14761 ctaagttgca tgatctcgac catcgttgaa gggttgctcg gcggcctggt acacagcatc
    14821 ctgatccgtc gcgggcgcac tgataaagtc tttaacccca ttaccgccgg tgccgtcacg
    14881 ttcgtcgctg aaatggtgca aatgttgatc atcctggcga tcgcccgacc ttatgaagat
    14941 gcggtgcgtc tggtgagtaa tattgctgcg ccaatgatgg ttaccaatac cgtcggcgcg
    15001 gcgctgttta tgcgtatatt gctcgataaa cgcgcgatgt ttgaaaaata cacttcggct
    15061 ttttctgcca ctgcgctaaa agtggctgcc tcgacggaag gcattttgcg ccaggggttt
    15121 aacgaagtga acagcatgaa agtggcacag gtgctgtatc aggagctgga tattggtgca
    15181 gtcgcgatta ccgatcgaga gaaattgctg gcctttaccg gaattggtga cgaccaccat
    15241 ttacccggca aaccgatttc ttcgacttac accttaaaag cgattgaaac cggtgaagtg
    15301 gtctacgctg atggcaacga agtaccttat cgttgctctt tgcatccgca atgcaaactg
    15361 gggtcgacgc tggtcattcc gttgcgtggc gaaaatcagc gggtgatggg caccatcaaa
    15421 ttgtatgaag ccaaaaaccg tttattcagt tcaatcaacc gcacgttggg cgaggggatt
    15481 gcgcaactgc tttcggcgca gatcctcgcc gggcaatatg agcggcaaaa agcgatgctc
    15541 acccagtcag agatcaaact gcttcacgcc caggtgaacc cccatttttt gtttaatgcg
    15601 cttaacacca ttaaagcggt gatccgccgc gacagcgaac aggccagcca gctggtccag
    15661 tatctttcca cttttttccg caaaaactta aagcggcctt cggagtttgt tactctcgca
    15721 gacgaaattg aacatgtgaa cgcttatctg caaattgaaa aggcgcgctt ccagtcgcgg
    15781 ttgcaggtca acattgctat tccgcaagaa ttatcccagc agcaattgcc cgcgtttacc
    15841 ctgcaaccga tagtggaaaa cgccattaaa catgggacat cacaactgct ggatacaggg
    15901 cgagtggcga tcagcgcccg acgtgagggg caacatttga tgctggagat cgaagacaat
    15961 gccggtttgt atcaaccggt aaccaatgcc agtgggctgg ggatgaatct ggtggataag
    16021 cgtttacgtg aacggtttgg cgatgactat gggataagcg tcgcctgtga gcctgattgt
    16081 tacacccgaa taacgttacg actaccatgg agggacgagg catgattaaa gtcttaattg
    16141 tcgatgatga accgcttgca cgggagaacc tgcgcgtatt tttgcaggag cagagcgata
    16201 ttgaaatcgt tggagagtgt tcaaacgccg tagaagggat cggcgcggtg cataaactgc
    16261 gcccggatgt gctgtttctc gatatccaga tgccgcgcat cagtggtctg gaaatggtgg
    16321 ggatgctcga ccccgaacat cgaccgtata tcgtttttct caccgcgttt gacgaatacg
    16381 ctattaaagc ctttgaagaa catgcctttg actatctgct gaagccaatt gatgaagcgc
    16441 gactggagaa aacgctggcg cgtttgcgtc aggaacgcag taagcaggat gtttcgctgt
    16501 taccggaaaa tcaacaggcg ctgaaattta tcccttgtac ggggcatagt cggatttatt
    16561 tactgcaaat gaaagatgtg gcatttgtca gcagtcggat gagcggtgtc tacgttacca
    16621 gccacgaagg gaaagagggc tttaccgaat tgacattacg taccctggaa agtcgtacac
    16681 cactactgcg ctgccatcgt cagtatctgg ttaacctcgc gcatttacag gagattcgtc
    16741 tggaagataa cggccaggcc gagttgattt tgcgtaatgg cttaaccgtg ccggtcagcc
    16801 gccgttatct gaaaagctta aaagaggcga ttggcctgta aaagactgct aaaatggctt
    16861 tttgcctcat caacacctga aggcctcatg ctaagtaacg atattctgcg cagcgtgcgc
    16921 tacattttga aagccaataa taatgacctg gtgcgtattc tggcgctggg taatgtcgaa
    16981 gccaccgcgg aacagatcgc cgtctggcta cgtaaagaag acgaagaggg ttttcagcgt
    17041 tgtccggaca ttgttttgtc gtcattcctc aatggcctga tttatgaaaa acgcggcaag
    17101 gatgagtctg ctccggcact ggagccggaa cgtcgcatta ataacaacat cgtgctgaaa
    17161 aaattacgca tcgcgttttc gctgaaaacc gatgacattc tggcgatcct caccgaacag
    17221 cagttccgcg tttcgatgcc ggaaattaca gcgatgatgc gcgcaccgga tcataaaaac
    17281 ttccgcgaat gcggcgatca atttttacgt tattttctgc gtggactggc agcgcgccag
    17341 catgtgaaga aaagctaaga cgggtatggc ggccatgcaa aaacatggcc gccgacagat
    17401 tatttcactt ctttaaaacc agcggctttc atcaccagtt ccatttgcgc catagtgata
    17461 ccttttttgg catcttcagc agaaacgttg attcctgaaa taccctgcag ggctttgaaa
    17521 tccacttttt ccatatcgat agtcacgttt tcctgcgcgt aagtatcggt ataggttaat
    17581 ttttcttcaa cacccgcgat gtttttgtat ttggcgctta acggctcaag tgtcctggca
    17641 gcgtcttctt tggtggttgc accaatggag gcaaattgaa ttttggtttc agaagattgc
    17701 ttaagcacct tgtcaccttt gtagacatag gtaatggcaa tttcagtacc gttcagattg
    17761 gcgctgaatt ttttcgattc ttctttgtca ccgcagccag caagagagaa aaccagaaca
    17821 gatgcaacaa cgagggaaaa cagcttattg aaagccttca tgtaaaactc cattttattt
    17881 aatcaaggaa ctggtgactc tcaccagggg ctatatagga tatgcctaat accgtggcgt
    17941 gagcagtccg gaactggagt agaactctta gtaaaaagca ctatttcatc cttgttgctg
    18001 aagcatgggg aataattgtt cgcacagcaa aacaccgtta ttcattgctt ctacccgtgc
    18061 ctcgctttct gtattacgaa attgtcccaa cacatgtgcc agccgataaa aacccaccgc
    18121 ggtgaggtca ttcgccagca actctgcctg accaatagca ctatgttcct gatagcgcca
    18181 gccgttatgg agcagttgaa taagtaacgc ctggcagcgc atcagcaact gatgagcagt
    18241 agacggcaca ggcagaacgc tggcagaagg tagtgatgcc accacaggcg cagtttctgc
    18301 gtccagcgcc caggcacgag tctttgtcat cattacctgt ggttccagtg tcaattgccc
    18361 ctctacaaag ctgacaaagc cagaaaccag acacacgggg tcgtctgttt gttgcaaaag
    18421 cgccgccatg cgttcaacgg cataaggcgc gctggctgag gctggcagtg ataacgtcag
    18481 cagattatct tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc ggctgctatc
    18541 ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga cctgattaag
    18601 aggccgtatg caggcggggg gacgctggcg taaatattcc cgcaaagcca caatgcccgg
    18661 ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc gacagcggca caacgctgct
    18721 taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg ctgcttgtga
    18781 aacaatttgc ccgcccgcca gtgcgccagc ctgaaaacta aacagccgac gcgtcgctgc
    18841 cggtgagttt tcctgttcgc ttctcggcca actacgcgaa agatgcaaaa tactgccggt
    18901 gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc agcaacgcat
    18961 acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc cagagccaac
    19021 gaccgtgcgc cacggcatgg gcggtacttc accgacactg tcacgtcgcg ctatctcttg
    19081 tgcactgttc aagcgactgg ttaacgccgc aagctggcac aagcattttc cggcatgata
    19141 gtggctggcg cgggcgtgga aggcatcaac gctggcgcgc agttgccgta gcgattcact
    19201 cacccatcgc cagttgcagc gttccgccgc ctgctgtgcg cggctgaaag cggcctcgta
    19261 atggattggc ggctggctga tgccgcccag ccataatgcc tgacttaatt gctgaacata
    19321 ttgacgacac gcgttgccct cttcgctggc aaacggatcg tcagatgatg tgacgtgttc
    19381 gctgcgcatc tgccagatta aatgagtaaa ttccgcttgc tgagttttgg cctcgacgaa
    19441 ggcctgcaca gccagtacga catgttcgca aagtgtgccg tcaatacaat cacagcgagc
    19501 gaaacgaatg ctgctgcggg aataaaaacg tacatcgctc atcggtaagc gggcagaggg
    19561 aatttcgccc ggcgtacaga acaactcaat ggtgatgcct ttagcgacca gcgcctgtgc
    19621 gcgtttgcgg gtggcatcgg gcagggtagc cagttcttcc agccagattg ccggatccca
    19681 ctcttcttct ttttccgtag gctgggtggt ggcgcaaagt cgctgataac ttaaaaccag
    19741 catcacgcga tggcggcaca tgccgctggc cccgcagctg cactgagcct ctttcagtgc
    19801 ctggccgttc gccagctggg tgcggacacc gtcactgaag gtggcgatta aagcgccgtt
    19861 ctcatgactg atttccggga cgttgccatt ttccagttct ttaaggctgc gcttaacaaa
    19921 accggcattg cttaacgccg tcagtgcctg tggtgtcagt tctaataatt ccggacgtag
    19981 tgaattcatg actgaagatt ctccgcaagc caggtcgcca gttcgcccgg cgtcatggcg
    20041 gctatttgtg caccgacatt aaccagtgcc tgggccatat cgcggtcata gcaaggtgtt
    20101 gcggtgctat cgagcgccgc cagtcccagc actttgatgc cgctctggac acactttttc
    20161 acctgatgcg tcagcaatga tgatgaacca ccttcataga aatcgctcac gaggataatg
    20221 acactttttg caggttgttc aataagttgc cgaccatact ccacggcact ggcgatattg
    20281 gtcccgccgc ccagttgtac tttcattaat aactctaccg gatcggcaac gtctgccgtg
    20341 agatcaacca cgctggtgtc aaacgccacc agatgggtac gaatgccggg taactgccat
    20401 aaacaggccg ccatcaccgc tgagtggatc accgagtcaa ccatcgatcc gctttgatca
    20461 accagtaaga ccagttgcca ttgatcgcta tggcgcttaa tgcggctgtt aaagcggggg
    20521 gattcgatat acaacttgcc gtgttgcgga tgccagtgtt gcaggttggc acgcagcgta
    20581 cttttgaaat cgaaattacg cgccagtgga ataaatgagc gacggcggcg atcgcggaca
    20641 ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc aacaacctgg
    20701 cgcactattc ggcgggcggc agccagcact tcgggattca tcagatgttt ggtgtgcaaa
    20761 acggcgcgta gcaggctttc agaaggctgc atacgttcca gcacgtcgag attcgtcacc
    20821 acatcttcaa tgccgtagcg cagtacggca tcgctttcca gtcgctcgat cacccgttgc
    20881 ggaaacagcg tgtgaatact gttgatccac tcaggggtgg tgagatttga gtcacctaat
    20941 ccaccggagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag ccactccagc
    21001 gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc tgccgcttcg
    21061 ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt cagttcagac
    21121 atggaaaact ccccagtgtt gcagcgatgc cagtgcctgc tgttcgagtt gttgatgatg
    21181 tgcaatgtct tgtggtggac aatgcaacgg catttgcagt gccgaaacgg gaagttgcac
    21241 cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg gtggtagcca
    21301 ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa tcggcatcac tcagttgatt
    21361 taaatgactg ctgaaaccgg cgataaatac cggctgacag gccagttgat gacgggctaa
    21421 tgccagcaaa ccgtgcagcg cctcgccgga ttgtgtcggg gataattgca ccagcattgt
    21481 cagcgccgct tcggcactgg cgttgggatg ttccagacgc atcagtgcgc caagtgccgc
    21541 gccgcgatcc agcgccgggg catgaatcgc ctgactgcgt cgctccagta aggctaccgc
    21601 cgcagaaaga gaaacgccgg gtaaattaac gccgctatgt agatcgcgca ggatatggca
    21661 aagcgcttgc cagctatgca ggtgagcgtg aaactccttt tcatccggtc tgccgttgga
    21721 ttcacatagc cacagcgtgc gatcgatggc cgcgcataat gtcgtttgta atatctgcgc
    21781 gccctgcata ccgctaattt catccagccg ccataaggca tatagcactt ccagcgcctg
    21841 gcccatttcg gcaaactgat tttcctgggc gattaataat gtgagttgct ccagtaattg
    21901 ttgattgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg tgatactgcc
    21961 gattccaccc gcgtccagca tatcggcttc taatttattg cgtgcggctt cctggagtgt
    22021 ggcaccaaag caggcggcct caattaatgc cgcatgttgg ctaagcgggc gggttaattt
    22081 ccagcgttct tcaccgttgc ctgcaagtgt cagtgtactt ccctgctggc gcacaacccc
    22141 tgggatctcc agtattacca gccgatgtaa cacctgactt tgcgccagtc catcgggggt
    22201 aaagcgattt aaagtaagct ccgccggaaa agagattcct gtacgttcca gttcgcaggt
    22261 gacatctttt ggcagaggcg gctgtggcgt agagggggca agtttaccga atccgtcacc
    22321 cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa taacgccgcg
    22381 atagctccac ggcaacggcg cattcagggc ttctttaatc agcgagcctg ctatggcatc
    22441 cagccagtca gtgcgtaacg gtagtgtatg accacgcaac tgtgccagtg ccatcgcatg
    22501 cagatgagcg gcagccatat ccgctgttga agcaggcaag tggtgctggc gcaaacgggt
    22561 aagaaccgtt ttcagcagtt gttcacctgc ctgctgcaag ccccaccgcc agcaccagtt
    22621 ttgccagacc ggggcaggca taccggaaag gtatcctgcc agcacatcaa ggcgcttttc
    22681 actgtagggg gtgagataac aacctgtaac ggcatctgcc agcgagggca attccggcgt
    22741 gttaatttcc tgcgggcatt cgcgccacat ttttgccagt gcagaagcgt gccagccacc
    22801 gcagacgact aacacgtcgc cattgttttg ctgcatcgcc caaccaatcc agcgggccat
    22861 aaagtcttcg cgctgacgat tgagagcatc tccgggggaa tcgccccgca gttgtgcaaa
    22921 atagtgcgcc agtgcagagg gtaatgcagt ttgctggctt tcatcttcga acaagtgatc
    22981 ccacagggtg tcgctgttat ccatgcgggt ggcacgcagc agcaaggtct ggctttcctc
    23041 ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca aatcgatgaa
    23101 gtaagtttgt gcctgaatac gacgagcggc ttgtagcgcc tgccactccg gcgaaaattc
    23161 agcatatggc gtccaggcac cacgcccggg ggctgcaccg tcctgatact ggcaataact
    23221 gtaaatagct accggaagct ggtgggctaa aaacagttcg tctacccggt cattaaaatc
    23281 agccgggcct tcaatcaaca cgtatcgtgg ccgctggctt tcgattaacg atttcaccag
    23341 ccgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc tcatgcatct
    23401 ttcctcacgg caggcggtgg cgagcttgat aataagcctg ccagtgagct tctttatgtg
    23461 tggcgactcg ctgttcgaag taacggcgca gacgagcgcg atcttcttcg ttatctttga
    23521 cgatggttcc ggcaatacag tccaccagat cggctggctc acctgcgcga ttcgctaaaa
    23581 accaggcgcg gacgcccact gcgtgggcca cgttcacggc ttcagcggtg gacataattg
    23641 ccgtcagggt atccattgac gttttctttt cgccgttggc acgcagatcg cggaaggtgc
    23701 ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta tgggccagca
    23761 aacgcgccga agcactggcg accagttcca gctcctgggc gaaatccata atcggaaaga
    23821 cggtttcaaa atcgaagcga cgctttagcg cggcactcat ctcattgacg ccgcgatcgc
    23881 gggtattggc agtggcaata atattaaagc cttctcgcgc atagagctga ctggcttcac
    23941 cggtgagttc cggcaccgtc attaccctgt cagaaagcat acctaacaga cagtcttgta
    24001 cttccagcgg tgtacgcgtt atctcttcaa aacggacaat tttgccgtcg cgcattccct
    24061 ggtaaagcgg ggcagggacc agcgcttccg ttgacggacc atggttgata agcagggcgt
    24121 agttccagcc gtacttgatt tgatcttcgg tggtagatgc gccgccctgg atggttaacc
    24181 cggcatcgcc gctaatagcg gttgccagta attcagacag gagcgatttt gcggtgccag
    24241 gttcccccac caacatcaaa ccgcgcccgg tagcgagagt gactaacatg cgttcgacag
    24301 cggagggact gatcacgact ttacggctaa tattttgcgc gctatcgcca aggataaaag
    24361 cgcgggccgc aggcaaactt aactgccaac cgggcgggca aggtgcgtga tcattttgtt
    24421 ttaattttgc cagttcatcg gcgtataaca cagcggcagg cggacgctgc agatgattat
    24481 tctgcggtga catatattca tccctgaaat gtattaatga aataaacgat attttacgcg
    24541 caataaatgt ttgccgcatt agttgtttga gcgaaaaata tctggaaagc agatcacagt
    24601 aaataaaaaa acctgctggt gaggcaggtt cttttattta ctacaatgac gctaacttgc
    24661 tgattgtgca acatttcgta ccaacgaata aattataacc atatccacac tccattgcaa
    24721 ttcagtgaat gagatttata atcctgcccg ttgggaggtt gtgccggatc ggaccaattt
    24781 taagtcctgg ctgctggacg ggtggtgccg caggcggtgc cctgatccgg agagcaacgt
    24841 ttcgtaccaa catacgtaaa ccagtatcga caggcagtgt aagggtaagg tgctggtgtt
    24901 gcgctcctta ataaggagag cggatggaca cgctgacaca aaagttaacc gtgctcattg
    24961 ctgtactgga gctattagtg gctctgttac ggttgattga tttgttgaag taacgggcaa
    25021 attgaaacgc atctaagggg gaataccgtt tcccccttta ataaaatgat tactgttttt
    25081 attaattcat catataactc atttttattt ataaattcat cgattttata atcaacgtac
    25141 tatttggccg ggatgtgaat atttatggtg ggctattggc gaaaaatatc tggaaagtag
    25201 atcgcagaaa ataaaagaac ctg
//
LOCUS       DAZGAP010000070        24986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGAP010000070 DAZGAP010000000
VERSION     DAZGAP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.070"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..782)
                     /locus_tag="ACHLAM_002352"
     CDS             complement(87..782)
                     /locus_tag="ACHLAM_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7853870.1"
                     /translation="MDGFSTERNNKGMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLDEIGVVFKYLGIDNVSFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERWVSTTKKASDCAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNDSCSVVG
                     KVIASQWPEETFS"
     gene            858..1073
                     /locus_tag="ACHLAM_002353"
     CDS             858..1073
                     /locus_tag="ACHLAM_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7853871.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVECLNKLGAKVSLDDVFPPEHKAA"
     gene            1193..1486
                     /locus_tag="ACHLAM_002354"
     CDS             1193..1486
                     /locus_tag="ACHLAM_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO7853872.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            1519..1665
                     /locus_tag="ACHLAM_002355"
     CDS             1519..1665
                     /locus_tag="ACHLAM_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HFO7853873.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            1658..2557
                     /locus_tag="ACHLAM_002356"
     CDS             1658..2557
                     /locus_tag="ACHLAM_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFO7853874.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTSKPAIPMPASNTVPADYQQ
                     ITTDKEYNNIISNTDVLESTAADKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDK
                     RKNQIRTFWRKAGVITRQLDGHGFTVQDWRNYLSYVGENCRWMFEERPNHQRGTVWHK
                     KGFDFLLNDNTYLKVREGEHDDR"
     gene            2547..3983
                     /locus_tag="ACHLAM_002357"
     CDS             2547..3983
                     /locus_tag="ACHLAM_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO7853875.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKILFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTAMQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDMLYIDDTPGLSLTQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            4366..4614
                     /locus_tag="ACHLAM_002358"
     CDS             4366..4614
                     /locus_tag="ACHLAM_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012987735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853876.1"
                     /translation="MTYPHILEIHEKAAAINAGRKPQFGDKMRNLVAGEDNPRRDAYF
                     VKQKRVTGRMNPGLWYTMTERKGNFWDSCPKGLIFIDE"
     gene            4626..4916
                     /locus_tag="ACHLAM_002359"
     CDS             4626..4916
                     /locus_tag="ACHLAM_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853877.1"
                     /translation="MDESRKQFEESWLRRGGESSYLIRYPENHHEIGSGDIGGQYVMD
                     DVQGHWQTWQASRAAIEIELPESFTMHSGRTPYLYVSEVQSAIRAAGVKVKE"
     gene            4919..5083
                     /locus_tag="ACHLAM_002360"
     CDS             4919..5083
                     /locus_tag="ACHLAM_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853878.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            5064..5504
                     /locus_tag="ACHLAM_002361"
     CDS             5064..5504
                     /locus_tag="ACHLAM_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7853879.1"
                     /translation="MRKLTFELRSTIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            5501..5677
                     /locus_tag="ACHLAM_002362"
     CDS             5501..5677
                     /locus_tag="ACHLAM_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7853880.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKQIPKESDVKTFNYTA
                     HLWDIRWLRERARK"
     gene            5680..6081
                     /locus_tag="ACHLAM_002363"
     CDS             5680..6081
                     /locus_tag="ACHLAM_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853881.1"
                     /translation="MLLIQPRFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            6041..6250
                     /locus_tag="ACHLAM_002364"
     CDS             6041..6250
                     /locus_tag="ACHLAM_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO7853882.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCADCCAELMSDPNSSMYEEEDDG"
     gene            6243..6854
                     /locus_tag="ACHLAM_002365"
     CDS             6243..6854
                     /locus_tag="ACHLAM_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7853883.1"
                     /translation="MAKPARRRCKNNECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            6851..7057
                     /locus_tag="ACHLAM_002366"
     CDS             6851..7057
                     /locus_tag="ACHLAM_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO7853884.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            7035..7706
                     /locus_tag="ACHLAM_002367"
     CDS             7035..7706
                     /locus_tag="ACHLAM_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO7853885.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLADRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFSNQMYIDTGAVFCGNLTLIQVQG
                     EGAWE"
     gene            7697..8215
                     /locus_tag="ACHLAM_002368"
     CDS             7697..8215
                     /locus_tag="ACHLAM_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7853886.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMCIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSLATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKSLDMQLSI"
     gene            8279..8353
                     /locus_tag="ACHLAM_002369"
     tRNA            8279..8353
                     /locus_tag="ACHLAM_002369"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8312..8314,aa:Asn,seq:gtt)
     gene            8359..8434
                     /locus_tag="ACHLAM_002370"
     tRNA            8359..8434
                     /locus_tag="ACHLAM_002370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8392..8394,aa:Thr,seq:tgt)
     gene            8677..9000
                     /locus_tag="ACHLAM_002371"
     CDS             8677..9000
                     /locus_tag="ACHLAM_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO7853887.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            8984..9460
                     /locus_tag="ACHLAM_002372"
     CDS             8984..9460
                     /locus_tag="ACHLAM_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7853888.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADNLIAKFKESGGTVREIEV"
     gene            9457..9924
                     /locus_tag="ACHLAM_002373"
     CDS             9457..9924
                     /locus_tag="ACHLAM_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7853889.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCSVPS
                     SAETSGASGMGNDSTVELSPVAGRNVLGVRDGIIRDQTALRTLQEYIRTQCLR"
     gene            9912..10064
                     /locus_tag="ACHLAM_002374"
     CDS             9912..10064
                     /locus_tag="ACHLAM_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853890.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGGHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            10270..10800
                     /locus_tag="ACHLAM_002375"
     CDS             10270..10800
                     /locus_tag="ACHLAM_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO7853891.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALEAKVQKSTLEQIQPLKIIKGGAEPGVWGVELLAIRYAAWIKPEF
                     EIEVYEVFKTVVRLGVGAMSRLNRIDHIINTETKAISQCASQMAKWGVGGRKRLLHVA
                     RERAANEVQMYLPGMV"
     gene            11057..11299
                     /locus_tag="ACHLAM_002376"
     CDS             11057..11299
                     /locus_tag="ACHLAM_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO7853892.1"
                     /translation="MAEITPMTEEQKFQIEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDTTTRTIEAVRKSKEALDLFTTGA"
     gene            11302..11742
                     /locus_tag="ACHLAM_002377"
     CDS             11302..11742
                     /locus_tag="ACHLAM_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO7853893.1"
                     /translation="MARPTKYQEAYAEQARKLCLLGYTDAELADFFEVSESTINKWKL
                     DYPEFSESIKKGKAVADAEVSDRLYQRAMGFVAPDIDIRVIENRIVETPLEKYYPPDT
                     TAAIFWLKNRQKDKWRDKVDHELTGKDGGAIQIETSPMSTLFGK"
     gene            11739..13154
                     /locus_tag="ACHLAM_002378"
     CDS             11739..13154
                     /locus_tag="ACHLAM_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO7853894.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQLFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKC
                     EWLEAETDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPESAAQPDYSSYSIPCGVG"
     gene            13156..15354
                     /locus_tag="ACHLAM_002379"
     CDS             13156..15354
                     /locus_tag="ACHLAM_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO7853895.1"
                     /translation="MAEKKMTDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGAQ
                     WEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEAL
                     AEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISLL
                     PVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQY
                     DWSTPDAIYVGRYYEVRIEKVKLAAWRNPVSGETAIYDEEQIKDIVDELTDGAFELIG
                     ERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAMD
                     AQRLENLMVSMIADNATQAGGDGIPIVDVDFIPGPLMNHWAERNKKRPAVLPMTSKKD
                     KNGTVISEAQVAGWTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVDS
                     IFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTGE
                     VVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDLV
                     SSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMVA
                     AQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIREA
                     LKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKSTQQQPQQ"
     gene            15445..16338
                     /locus_tag="ACHLAM_002380"
     CDS             15445..16338
                     /locus_tag="ACHLAM_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFO7853896.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGGESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDSAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            16357..17610
                     /locus_tag="ACHLAM_002381"
     CDS             16357..17610
                     /locus_tag="ACHLAM_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO7853897.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RVPKGTATSITLAAAPAHKVEAKDANDMPVDNRQGTITVSAEGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPPDNADVASRPYANVDANAASSAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            17652..17840
                     /locus_tag="ACHLAM_002382"
     CDS             17652..17840
                     /locus_tag="ACHLAM_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853898.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            17821..18282
                     /locus_tag="ACHLAM_002383"
     CDS             17821..18282
                     /locus_tag="ACHLAM_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO7853899.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            18292..19710
                     /locus_tag="ACHLAM_002384"
     CDS             18292..19710
                     /locus_tag="ACHLAM_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO7853900.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNI
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            19710..20558
                     /locus_tag="ACHLAM_002385"
     CDS             19710..20558
                     /locus_tag="ACHLAM_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO7853901.1"
                     /translation="MADSNLNEPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLMPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            20558..21037
                     /locus_tag="ACHLAM_002386"
     CDS             20558..21037
                     /locus_tag="ACHLAM_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO7853902.1"
                     /translation="MEIKLIDNPVKLAEFLNNPANTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYTSNGKPVGITIYQLPRSKYEELKNVNFPDCQ"
     gene            21012..21665
                     /locus_tag="ACHLAM_002387"
     CDS             21012..21665
                     /locus_tag="ACHLAM_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO7853903.1"
                     /translation="MLIFQIANKHLSKAVYCKGGSDSGAKEQARATEKGIELQREMWQ
                     TNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAAAE
                     ATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYANNV
                     SQLYQQQAAASAANANKPSGFQSALGGAAAGAAAGTAIMPGWGTAIGAGVGLLGSLF"
     gene            21678..23150
                     /locus_tag="ACHLAM_002388"
     CDS             21678..23150
                     /locus_tag="ACHLAM_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO7853904.1"
                     /translation="MATWQQAGNSGALLAGLGGMNSNAPRASDADATLAYIRQNNEME
                     RSGRNNVGLQALQGISSVMDMYKQMDQQKRQQEFQQAYADAYTSGDRDAMRKLASQYP
                     EQFDAVRNGMKFVDEDQRSTVGTLAASARLAASSPEAMMSWLQNNSSELTRAGVDPLD
                     VAKMYQQNPQGFTEFVDHLGMAALGPEQYFQAQDRIVGREIDRGRLAETIRSNQAGEA
                     LQARGQDISRANALTSAYAPTAAMQNYNQYAQMLKADPDGAAAFAAAAGINPNAKKLL
                     KVETNPDGSVTKYYTDGSEEAGKLNQPISGDGIKPISLPQAQSIIDKANEGSKKAAGF
                     ALRLKDSMDSMNQLSKSIDPKRVALINRSLGDGTIANLSLSPAEQQYMVNARDALYAI
                     LRPETGAAITLPEMQEYSKMYLPQPGDSKAATETKMRKMQGQYNSLRGQSGRVYDALV
                     VSSAANSQQQSNSQQPTNTQQQQSQSGSYTSKSGIQFTVE"
     gene            23150..>24986
                     /locus_tag="ACHLAM_002389"
     CDS             23150..>24986
                     /locus_tag="ACHLAM_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO7853905.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     EPAREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTITLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNAAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNQAATAVRPLLANEATRQ"
BASE COUNT         7157 a         5758 c         6399 g         5672 t
ORIGIN      
        1 ttgtatttaa tgggtttata ttttggaaaa agaaaacccg acgctaaggt cgggttattg
       61 ttgtgtattt tagagttgtg aggctgttaa ctaaatgtct cttcaggcca ctggctggcg
      121 ataactttcc ctactacgga acagctatca ttgcatggaa tcattggata ttgcgggttt
      181 agtggctgta ggaacacctg accgctatcc ctgatcagtt tcttgaaggt aaactcgtca
      241 ccaccaagtc tggctatgca gaaatcacct ggctcaacag cctgctcagg gtcaacgaga
      301 attaacatcc cgtcaggaaa gcttggcttg gatcctgttg gcgcggtcat ggaattacct
      361 tcaacttcaa gccagaacgc acaatcactg gcttttttgg ttgtgctgac ccatctctcc
      421 gcatcacctt tggtaaaggt tctaagctca ggcgagaaca tcccggcctg aacatgagaa
      481 aaaacagggt actcatattg ttttttaacg ggggcagatg agtattcgcc aacaggtgaa
      541 aatgtaccgt cgtggttgaa tgagacgtta tcaataccaa ggtatttaaa caccacacca
      601 atctcgtcaa gagatggatg acgagatccg cgcaaccagt gaccaattcc accctgcgtc
      661 ataccaagct cttcagctaa cttctcttga gttatgccta gctctttcat tctggatcta
      721 gccagttcat accatttcat tttcataccc ttattattac gctctgtact aaaaccatcc
      781 atgcacaaga tgtatttttt gtttgcattc taaaagtaca tatcgtatta ttgtttcatg
      841 gttactatgg agggcatatg agcaacctac gaaaatatcg agagtcactg aatatctctc
      901 aaacaacact tgctaaggca gttggatgca cacagggagc tatcggacat tgggaatctg
      961 gtcgtcgctt cccagacctt aaaacatgcc gtgctcttgt tgagtgccta aacaagttag
     1021 gcgcaaaagt cagtcttgat gacgtgttcc cgccggaaca caaagccgct taagacattc
     1081 ccgctcttac acatcccagc cctgaaaaag ggcatcaaat taaaccacac ctatggtgta
     1141 tgcatttatt tgcatacatt caatcaattg ttatctaagg aaatacttac atatggttcg
     1201 tgcaaacaaa cgcaacgagg ctctacgaat cgagagtgcg ttgcttaaca aaatcgcaat
     1261 gcttggaact gagaagacag cggaagctgt gggagttgat aagtcgcaga tcagcaggtg
     1321 gaagagggat tggattccaa agttctcaat gctgcttgct gttcttgaat ggggtgtcgt
     1381 tgacgacgac atggctcgat tggcacgaca agttgcttcg attctcacca ataaaaaacg
     1441 cccggcggca accgagcgtt ctgaacaaat ccagatggaa ttctgaggtc attactggat
     1501 caatccacag gagtcattat gacaaaacaa ctcagtcctt accaggacaa aattcacaaa
     1561 cacatactac gtgatcgctt cctgtccagc ttcaagcagc ctggtcgatt ccgggctgag
     1621 ttggaaaaag tgaagctgat gcagaaggag aaaggtcatg agtaacatat ctaatctagc
     1681 cgaagccaga gaggccagaa ggctacaaca accgcatcaa agcagcggta aggggtatgc
     1741 cttgctgcac cgtaaaatta tggatgtgcc gttttacaag gacgcagaag ctgcgcatct
     1801 gtgggttcac ttaatcctca aagcaaagca tacgcctgag tatgtaatga ctgacgcagg
     1861 agaaattctg gtaggcagag ggaagctact tggcggtaga aactctctgg cgtttgaaac
     1921 aggactcaaa ccagatcgcg ttcagtacct gcttagaaag ttcaaaaaac tcggcatgat
     1981 tgactgggtt tcacacggta aattctcagt tttctcggta gagaaatatg acgattatca
     2041 gtcaaatttt gtaccagcag attaccagca aatcaccacc tcaaagccag caataccaat
     2101 gcctgcaagc aatactgtac cagcagatta ccagcaaatt accacagata aagaatataa
     2161 taatattatc tctaatactg acgtattaga gagtaccgca gcagacaaaa agtctgacaa
     2221 gaaaaaacct tccgtcagct gtcaggatgt tgtcgatgct taccacgaaa tccttcctga
     2281 agcgccaaga atccgcgcac tgaatgacaa gcgtaaaaac cagatccgaa cgttctggcg
     2341 caaagccgga gtgataaccc gccagcttga cgggcatggg ttcacggtgc aggactggag
     2401 aaattatttg agctacgtag gcgaaaattg ccgatggatg ttcgaagagc gcccaaacca
     2461 tcagcgcgga accgtctggc acaaaaaggg atttgatttc ctgcttaacg ataataccta
     2521 cctgaaagtt cgtgagggtg aacacgatga ccgataattt ttatgcgccg ccccatagca
     2581 tcgaggcaga gcaggcggtg attggtggat tgcttctgga tgatgacagc agtgagcgcg
     2641 tccagaaagt tctggcgatg ctgaagcccg attcatttta cagccgacca cacaaaatcc
     2701 ttttcgaaga aataaccaga atgcaccggg agcaaaagcc ggtagatggc ctgacgcttt
     2761 tcgatgaact ggagcgtaaa tcgttaacgg cgtctgttgg cggttttgct tatatcgctg
     2821 agatcgcaaa gaacactcca agcgcagcaa acatcgttgc ctatgcaatg caggttcgtg
     2881 aaaccgcaat ggaacgctac gccatcaacc gcatgactga agcgacggaa ttgctctatt
     2941 cccgcaacgg aatgactgca atgcagaagt acgaagctat tcaggcgatt ttcacgcaac
     3001 tgacagacca tgcaaaaacc ggatcgcgtc gcggccttcg ctcatttggt gaggtcatgg
     3061 aagactgggt tagcgacctt gagaagcgat ttgacccgtc aggcgaacaa cgaggaatga
     3121 gcacagggat cccatcgctg gacaggatgc tgtcaccgaa aggtctggtg aaaggctctc
     3181 tgtttgtcat tggcgctcgc cctaagatgg ggaaaacgac gctatacagc cagatggcaa
     3241 tcaactgcgc agtgcatgag aaaaagcccg ctctgatgtt cagccttgaa atgccaggtg
     3301 atcagatact ggaaaaactg gtagggcaga agtctggtgt taatccgaat attttttacc
     3361 ttccggcgac aaatgacgct gatgacggct atcagggtga ttacgatggt gacttcaaca
     3421 gggcgataga aacagcaaat cgcttgagtg aaatcgacat gctttacatc gacgacacgc
     3481 cgggattatc tctgactcaa atcgtcagcg aaagccgtcg aatcaagcga gaaaaaggat
     3541 gtgttggcat gattctggtc gattacctga cactaatgac cgctgagaag gccgatcgca
     3601 acgaccttgc ttacggcatg atcaccaaag gactgaagaa ccttgccaaa gagcttgatt
     3661 gcgttgttgt gcttctgaca cagcttaacc gcgcactgga aagccgaacc aataaacgcc
     3721 cattaccaag tgactcacga gatacagggc agattgaaca ggattgcgat tattgggtcg
     3781 ggatccatcg tgaaggcgct tttgatgaca gtgttccacc tggtgaaact gaactaatcc
     3841 ttcgtctcaa tcgtcatggc aataccggca cggtgtattg cattcaggca aatggcgcta
     3901 tttatgacac agaccaacag tccgctgaaa tgcgccgccg tgaacgcgag gaaccgcaat
     3961 ccaagaagaa aggaggattc tgatgaccat ctacatcact gagctaataa caggggctat
     4021 ttacacagta gccctttttt attggatcgg aagcgagagg tgagagtgtg agcgagccga
     4081 ttatgtctcc tgacctttat ttaaaatata ggtctatttg ggggcacaga aaaatgaaag
     4141 ttaatagtta tagattcgac gatgcaactc aatgggtaac taatttcgga aaggtaagaa
     4201 taactccgcc gcagtttaga aaactccttt gcaacaaatg cggaagcgat aactggacag
     4261 atgatggaag aagcatgaat gagtactgct gcgactcatg cggtcagtac gtgatggttc
     4321 agggcgacga cgcttaacac cccagcacgc tgatggagag gaattatgac atatccgcac
     4381 attttagaaa ttcacgaaaa ggcggcagcg attaacgcag gacgaaagcc tcagtttggg
     4441 gacaaaatgc gaaaccttgt ggccggtgaa gacaacccgc gccgcgatgc ctacttcgtg
     4501 aaacagaaaa gagtgacagg acgaatgaat cccgggctct ggtacacgat gacagaacgc
     4561 aaaggtaact tctgggactc ctgtccgaaa ggcctgattt tcattgacga gtgatggaga
     4621 ggaatatgga cgaatcaaga aagcagtttg aagaaagttg gttgcgacgt ggaggcgaat
     4681 cttcatacct tatccgttac cctgaaaatc accatgaaat tggcagtggt gatattggtg
     4741 gtcaatacgt gatggacgat gttcaaggcc actggcaaac gtggcaggca tcgcgagcag
     4801 ctattgaaat tgagttgcct gaaagcttta ccatgcatag tgggcgaacc ccatatctgt
     4861 atgtaagcga ggtgcagagc gccatccgcg ctgctggagt caaagtgaag gagtgagtat
     4921 gagcgcatac gaagaaatca tgttagccct gcgattcttt ttcggtgtgg aagaagatga
     4981 aaacgtaaat gagattatcg ggcaagacca tgacccgata gggactattg cagctgcact
     5041 tgacgattac aggagcgtaa atggtgagga aactaacgtt tgaactaaga agcaccatcc
     5101 atcagcagaa cgccattcaa gctatccagc aaatccttcc agacccaacc aaaccaatcg
     5161 tagtgaccat ccaggaacga aaccgtagct tagaccaaaa ccgaaagctt tgggcttgcc
     5221 ttggtgacgt ctctcgtcag gttgaatggc atggtcgctg gctggatgca gaaagctgga
     5281 agtgtgtgtt taccgcagca ttaaagcagc aggatgttgt tcctaacctt gccgggaatg
     5341 gctttgtggt aataggccag tcaaccagca ggatgcgtgt aggcgaattt gcggagctat
     5401 tagagcttat acaggcattc ggtacagagc gtggcgttaa gtggtcagac gaagcgcgac
     5461 tggctctgga gtggaaagcg cgatggggag acagggcagc atgagacgac agcgacgaag
     5521 tatcaccgac atcatctgcg aaaactgcaa ataccttcca acgaaacgct ccagaaataa
     5581 acgcaagcaa atcccaaaag aatctgacgt aaaaaccttc aactacacgg ctcacctgtg
     5641 ggatatccgg tggcttagag aacgcgcgag gaaatgacaa tgcttttaat tcaacctaga
     5701 tttggcctta gcatcaaaaa agggcacatg tttggtgaga aagagtcaca acgaaaaatg
     5761 gtgtctatcc ggttgccatt tatcagtatt tattggctaa acagggaggc aacaaattat
     5821 tggtatacat gcgccagagc agcatttaac gaccctgact ggtttgtgaa aaaccaccac
     5881 gcagttcgtc aggcaaagag aaaggccaat acgacataca tgaaggcgta tcgaaaagca
     5941 tggaaagaac accgcgatcg ataccagcaa gacatggaaa agcttgaatc agaaaacatg
     6001 gaattaagac gaaagcttgg tgaagcaaaa cgagacattg atgcttacaa gcgacttttt
     6061 aatggtgaaa gccatgctta gtccatccca atctcttcaa taccagaaag aaagcgtcga
     6121 gcgggcttta acgtgcgcta actgcggtca gaagctgcac gtgctggaag ttcacgtgtg
     6181 cgccgattgc tgcgcagaac tgatgagcga tccgaatagc tcaatgtacg aggaagaaga
     6241 cgatggctaa accagcgcga agacgatgta aaaacaatga atgtcgggaa tggtttcacc
     6301 ctgcattcgc taatcagtgg tggtgctctc cagagtgtgg aaccaagata gcactcgaac
     6361 gacgaagtaa agaacgcgaa aaagcggaaa aagcagcaga gaagaaacga cgacgagagg
     6421 agcagaaaca gaaagataaa cttaagattc gaaaactcgc cttaaagccc cgcagttact
     6481 ggattaaaca agcccaacaa gccgtaaacg ccttcatcag agaaagagac cgcgacttac
     6541 catgtatctc gtgcggaacg ctcacgtctg ctcagtggga tgccggacat taccggacaa
     6601 ctgctgcggc acctcaactc cgatttgatg aacgcaatat tcacaagcaa tgcgtggtgt
     6661 gcaaccagca caaaagcgga aatctcgttc cgtatcgcgt cgaactgatt agccgcatcg
     6721 ggcaggaagc agtagacgaa atcgaatcaa accataaccg tcatcgctgg actatcgaag
     6781 agtgcaaggc gatcaaggca gagtaccaac agaaactcaa agacctgcga aatagcagaa
     6841 gtgaggctgc atgacgttct cagtaaaaac cattccagac atgctcgttg aagcatacgg
     6901 aaaccagaca gaagtagcac gcagactgaa atgtagtcgc ggtacggtca gaaaatacgt
     6961 tgatgataaa gacgggaaaa tgcacgccat cgtcaacgac gttctcatgg ttcatcgcgg
     7021 atggagtgaa agagatgcgc tattacgaaa gaattgatgg cagcaaatac cgaaatattt
     7081 gggtagttgg cgatctgcac ggatgctaca cgaacctgat gaaaaaactg gagacgatag
     7141 gattcgacac caaaaaagac ctgcttatct cggttggcga tttggccgat cgcggtacag
     7201 agaacgttga atgcctggaa ttaatcacat tcccctggtt cagagctgta cgtggaaacc
     7261 atgagcaaat gatgattgat ggcttatcag aacgcggaaa cgtcaatcac tggctgctta
     7321 atggcggtgg ctggttcttt aatctcgatt acgacaaaga aattctggct aaagctcttg
     7381 cccataaagc agatgaactt ccgttaatca tcgaactggt gagtaaaggt aaaaaatatg
     7441 tcatctgcca cgccgattat ccttgtgacg aatacgagtt tggaaagcca gttgatcatc
     7501 agcaggtaat ctggaaccgc gaacgaatca gcaactcaca agacggaatc gtgaaagaaa
     7561 tcaaaggagc ggacacgttc atctttggtc atacgccagc agtgaaacca ctcaagtttt
     7621 ccaaccagat gtatatcgat accggcgcag tgttctgcgg aaatctcaca ttgattcagg
     7681 tacagggaga aggcgcatgg gaataagaga actaaacctc accaaagagc agcacgagtg
     7741 gctgaatggc tggcttgaac tgtggggcgc atgggtttat tcaggtcgtc tggaaaagcg
     7801 catgagcagc gtaatagcga agttcatgga gagcgtagag ccgggaagag ttatgacaag
     7861 gccaatgtgc aatgatgatg atggaatgtt gatttctcag gtcgtcgatt ccgtcatgtg
     7921 cattgacaag aaagcctttg gcatcctcct cagctactac gctcatggtt catctaagcg
     7981 agcaattgca tcctactatc acgcgactgc aaagccacgc aagatgtgtg gacgtggtgg
     8041 cgagggatgg agaaaacctt cactggcaac ctgtagaaac gaaattgacg acatcctgaa
     8101 agcgtcgtta tttgttttgt accagccaat gcaaaatgct ttcaaaatgc gtaaacgtgt
     8161 tgagaaagtt aagcatgttg ctgttaaaag ccttgacatg caattatcca tttagccata
     8221 attagaaggt aagctgccgt tagtgactct taagttgcaa cggtggcttt ttttatttgg
     8281 gtaagtcgta taaaggtcat tacggaaggc tgttaacctt cttatcgtgg ttcgagccca
     8341 tgctgtcccg ccaaatatgc tggtttagct ccaatggtag agcagtcgcc ttgtaagcga
     8401 atgggtagcg gttcaagtcc gttaaccagc accataactg agccgtagcc actggctatc
     8461 ctgaattcat cagtgatagt tatgctgcgg ccttctacac atgaccttcg tgaaagcggg
     8521 tggcaagaag ttgcgctaac aacctcctgc cgttttgccc gtgcatatcg gtcacgaaca
     8581 aatctgatta ctaaacacag tagcctggat ttgttctatc agtaatcgac cttattccta
     8641 attaaataga gcaaatcccc ttattggggg taagacatga agatgccaga aaaacatgac
     8701 ctgttagccg ccattctcgc ggcaaaggaa caaggcatcg gggcaatcct tgcgtttgca
     8761 atggcgtacc ttcgcggcag atataatggc ggtgcgttta caaaaacagt aatcgacgca
     8821 acgatgtgcg ccattatcgc ctggttcatt cgtgaccttc tcgacttcgc cggactaagt
     8881 agcaatctcg cttatataac gagcgtgttc atcggctaca tcggtactga ctcgattggt
     8941 tcgcttatca aacgcttcgc tgctaaaaaa gccggagtag aagatggtgg aaatcaataa
     9001 tcaacgtaag gcgttcctcg atatgctggc gtggtcagag ggaactgata acggacgtca
     9061 gaaaaccaga aatcatggtt atgacgtcat tgttggcgga gagctattta ccgattactc
     9121 cgatcaccct cgcaaacttg tcacgctaaa cccaaaactc aaatcaacag cagcaggacg
     9181 ttaccagctt ctttcccgtt ggtgggatgc ttaccgtaag cagcttggcc tgaaagactt
     9241 ctctccgaaa agccaggacg ctgtggcatt gcagcagatt aaagagcgtg gcgctttgcc
     9301 gatgattgat cgcggtgata tccgtcaggc tatcgaccgt tgcagcaata tctgggcttc
     9361 actgccgggc gctggttatg gtcagttcga gcataaggct gacaacctga ttgcaaaatt
     9421 caaagagtct ggcggaacgg tcagagagat tgaggtatga gcagagtcac cgcgattatc
     9481 tccgctctgg ttatctgcat catcgtctgc ctgtcatggg ctgttaatca ttaccgtgat
     9541 aatgcaatcg cctacaaaga acagcgcgat aaaaaagtca gtgagctgaa gcaggcgacc
     9601 gccaccatta ctgacatgca gcagcgccag cgtgatgctg atgcacttga tgctaaatac
     9661 acgaaggagt tagctgatgc gaaagctgaa aatgatgctc ttcggcgcaa gcttgataat
     9721 ggtggccggg tgctcgtcaa aggaaaatgc tctgtgccat cctcagccga aacctccggc
     9781 gcctccggca tgggcaatga ttccaccgtc gaactctctc cagttgctgg acgaaacgtt
     9841 ctcggtgtcc gagacggaat tatccgcgac caaacagcac tgagaacgct tcaggaatat
     9901 atcaggacgc aatgccttcg atgatagcga taattttact catcatcctt cacatctggc
     9961 tctgtagaca gggtggtggt cacttctgga gtaaatccag attaaatatc tcattgttga
    10021 tgcttgatat tgagcatctg gcgcgcggta aggggctgcg ttgagataag agcctgtcat
    10081 cacaaacacc aggatttagc ctcgcattcg cggggctttt ttatatctgc aacaaacgcg
    10141 cttcacacgc gcgacttctg aacacagaac ctttcaggat gacccttgag gatgccggtt
    10201 tggtgatcgg tgcctttctg tgggccggaa tcctgtgtga caaggttcat cactaaaagg
    10261 taaccactga tgaagtaccc aacagttatt gtcaatggtg tgtccgttcg tgttgatgag
    10321 gatggacgct acaacttaaa cgatctccat gcagcagcag ttgcaaatgg agaggctaca
    10381 gagcaacagc gcccaagcca gtttttgcgt agcgcgcaga taaaacgctt cataaaagca
    10441 ctggaggcca aagtgcaaaa aagcactttg gaacaaattc aaccacttaa aataatcaaa
    10501 ggtggtgcgg aaccaggtgt gtggggtgtt gaacttctgg caatcagata tgcagcatgg
    10561 attaagccgg aatttgaaat cgaagtttat gaagttttca aaacggttgt ccgtctcggc
    10621 gttggcgcaa tgtcccgtct gaatagaatc gatcacatca tcaatactga aaccaaagcg
    10681 ataagccagt gcgcaagcca aatggctaag tggggcgttg gtgggcgaaa aagattgctt
    10741 catgttgcac gtgagagagc ggcaaatgaa gtgcaaatgt atttgcccgg aatggtgtga
    10801 tttcgcaggt taatccagtt tgtacattac ggcagtacca cgaagcaacc caagccagaa
    10861 agtggggaaa taacactggc agccactgaa agatgaacct ccagccttat ggcaaaaaag
    10921 attctttgtg gtggcggact gatggaaaga catcggttat tgcagagacc attcaatgag
    10981 tggtctcgac aatggcttat accttacacg ggataactta actgatatcc cttttaacgg
    11041 ataaacggag ccaacaatgg cagagattac ccccatgact gaagaacaga aattccagat
    11101 agagatttac aaactggtca tgaaccagaa cgcagccgca gaggaagcat ttcagttcat
    11161 tggcactgac gaactgaagc ttgagctatt caaaattcac ttccagtcag gcggcgctaa
    11221 ttcagatacc acgacccgca ctatcgaagc ggtgcgtaaa tcgaaggaag cgttagacct
    11281 gttcactacc ggagcataaa catggcgcgc ccaacaaagt atcaagaggc gtacgccgaa
    11341 caggcacgca aactgtgctt gctgggctac accgatgcag agcttgctga tttcttcgaa
    11401 gtcagtgagt caactattaa caagtggaag cttgattatc ctgagttttc ggagtccata
    11461 aaaaagggta aggccgtcgc tgatgcagaa gttagtgacc gtctttatca acgcgctatg
    11521 ggcttcgtgg ccccagacat tgatattcgt gttattgaaa acagaattgt cgaaactccg
    11581 cttgagaagt attacccgcc ggatacaaca gctgccattt tctggcttaa gaaccgacag
    11641 aaggataaat ggcgcgacaa ggttgatcac gagctaacag gcaaagacgg cggcgcaatc
    11701 cagattgaaa catcaccgat gagcactcta tttgggaaat gacctcgatt aatcctatct
    11761 ttgaaccgtt cattgaggcg catcgctata aagtcgccaa aggcggtcga ggtagcggta
    11821 agtcatgggc aattgcgaga ctgcttgttg aagcggcgcg tcggcagccg gtgcgtattc
    11881 tctgcgctcg tgaactgcaa aacagtatca gcgattcggt aatccggttg cttgaagaca
    11941 ccatagagcg ggaagggtat tcggctgagt ttgaaattca gcgttcaatg attcgtcatc
    12001 tcggaacgaa tgctgaattc atgttctacg gcataaaaaa caaccctacg aagattaaat
    12061 cgctcgaagg cattgatatc tgctgggtgg aagaagcgga agcggtaaca aaggaatcgt
    12121 gggatattct gatcccaacc atccgtaagc cgttctctga aatatgggtg agcttcaacc
    12181 cgaaaaacat cctcgacgat acctatcagc tattcgtcgt aaatcctccc gatgatattt
    12241 gcctgctgac ggtcaactac accgacaatc cgcactttcc tgaagttctc cgtctggaga
    12301 tggaagagtg taaacgcaga aacccgacac tgtatcgtca catctggctt ggtgagccag
    12361 taagcgcaag tgatatggca atcatcaaat gtgaatggct tgaagccgaa accgatgcgc
    12421 acaagaaact cggatggaaa gcgaaaggcg cggttgtttc tgcacatgac ccatcagata
    12481 cagggccaga tgctaaaggt tatgcatcgc gtcacggttc ggtagttaag cgcattgccg
    12541 aaggtctgct gatggacatc aacgagggtg ctgactgggc tacttcgctg gcgattgaag
    12601 acggcgctga ccactacctg tgggatggtg atggtgttgg tgccgggcta cgcagacaga
    12661 caacggaagc gttctccggc aagaaaataa ccgccacgat gttcaagggc agcgaatcgc
    12721 cattcgatga agatgcgcct tatcaggccg gggcatgggc tgatgaagtc gtacagggtg
    12781 acaacgttcg cactattggc gatgtgttcc gcaataagcg agcgcaattc tattacgcac
    12841 tggctgacag gctgtatctg acatatcggg cggttgtcca tggggagtat gcagatcctg
    12901 acgacatgct gagtttcgac aaagaagcga taggcgagaa gatgctggag aagctgtttg
    12961 cagaactgac gcagattcag cgcaaattca ataacaacgg gaagctggag cttatgacta
    13021 aggtcgaaat gaagcagaag ctcggtattc catctcctaa cctggctgat gcgctgatga
    13081 tgtgtatgca ttgcccggag tcggctgcgc aacccgacta ttccagttac tcaattcctt
    13141 gtggtgtagg ttgatatggc agaaaaaaag atgactgact ggcatcgcaa ggtgctgtgc
    13201 aactttgata atgcctggtc agcaacgcag gatatgcgtg agcagattat tgaggctcag
    13261 cgtttcgtcc gggtgtccgg cgcacagtgg gaaggcagca caaacgctgg ttactcattt
    13321 gatgaaggca ggtttgagca ttatccgcgt tttgaactga ataagattgc ccgtgaatgt
    13381 gatcgcatca ttggcgagta tcgacagaat cgcatcagcg ttaaattcag gccgaaggat
    13441 gacaaggcat cggaagcgtt agccgaaaaa atgaacggca aattccgcgc tgactatcag
    13501 gaaacatccg gtggcgaagc gtgtgataac gcatttgatg atgctgtaac gggcggattc
    13561 ggttgtttcc gcatgtgtgc cgattacgaa gatgaaatgg atccgagtaa cgagcagcgc
    13621 cgcatcagcc ttcttcctgt ttacgaccca gcgacatgcg tattcttcga tcaggacagc
    13681 aagcaatatg accgctctga tgctatgtgg gctatggaaa tgttctccat gacgcctaaa
    13741 gcgttcgagg ctgaataccc tgattccatc gcggcaagtc tttctcgtga tgacactggt
    13801 actcagtatg actggtcaac gcctgacgct atctatgttg gacgttacta cgaagtccgc
    13861 atagagaagg tgaagctcgc agcatggcgt aaccctgtca gcggagaaac ggcaatctat
    13921 gatgaagagc aaatcaaaga tattgtcgac gagctgaccg atggtgcatt cgaactgatt
    13981 ggcgagcgaa cggtgaagaa acgccgagtt tattgcggtc ttctgtctgg cgctgaatgg
    14041 ctggaagaac ctaagcgtat tccgggcgaa catattcctc tcatcccggt atatgggcgt
    14101 cgttcatttg ttgataatca ggagcgaatc gaaggccacg cagcaaaagc gatggatgca
    14161 cagcgtcttg agaacctgat ggtttccatg attgcagata acgctactca ggctggcggt
    14221 gatggcattc ctatcgtgga tgttgatttc attcccggtc cattaatgaa tcactgggca
    14281 gagaggaata agaaaagacc tgcagttctt cccatgacca gcaagaagga caaaaacgga
    14341 acggtcattt cagaggctca ggttgctggc tggacacctc cgacacaaat gcctcctgct
    14401 cttgccgggc tattgcagta caccgggacg gctattcagc aaattacagg tgcgtcgcag
    14461 cttgagaaca tgccgagcaa cgtcgctacc gataccgttg atagcatctt taaccggatg
    14521 gacacgcagt cctatatcta catggacaac atggctaaat ccatgcgccg tgctggcgtc
    14581 gtgtggcttt ctatggctcg tgaagtctat ggcagcgata cgccaatgcg catcgttaat
    14641 gaggatggca gcgatgacgt ggcgctgatg actggtgaag tggttgaccg tcagacaggg
    14701 caggttatcg cgcttaacga cctttcgcag ggtaactatg aagtgactgt cgatgtcggc
    14761 cagtcgttcg ctactcgccg tgatgcaacg gttaagtcgt tactttccat gctggcactt
    14821 atcccaccag gaacgccgaa gcacgacctt gtatcgtcga tgattctcga caatatggac
    14881 ggcgaaggga tggacgacct taaagaatac aaccgcaatc agttgcttct gtctggagtt
    14941 atcaagccga gaacgccaga agaacagcag atggttgagc aggcgaaaca acaacaggcc
    15001 agtcagccag atcctgctat ggttgctgcg caaggtcagc ttcttgctgg tcaggctgaa
    15061 ttgcagaaag cgcagaacga acaggcagcc attcaggtta aagcattcca ggcacagact
    15121 gatgctcagg ttgcagcggc aaatgttgtg aaaatcctcg catctgccga tagccagcag
    15181 aaatctgata tccgcgaggc tctgaaactg ctcggacagt tccagcaaca gcaaggagac
    15241 aatgcccgtg ctgatgcaga gcttgtcctg aaaagtcagg cacagggcca tgcgcagcgc
    15301 atggacatca gcagcatcct gcaaaaatca actcagcaac aaccacagca gtaattaacc
    15361 cataacgtgc aatggctgtc tttatgaggc ctggcaccct attgccttcc gatgggctga
    15421 acatcgagta aacaggggta acaaatggac cagatggcag aaaacacacc agaagttgaa
    15481 atcgaaaccg acgcgtcaga gcagattcct gatgatgtcg aactggctga agaagtcgaa
    15541 acagaagatg gcggtgagtc ctctggcaat gatgcagagg aagctactga aactgatgac
    15601 gacgaatcag aacaggaatt ctactttggt gacgaaaagc tggattcgcc aaccagcgaa
    15661 gatagcgcag agcatggact ggtaaaacac ctgcgcaaga cgattaaaga gaaagaccgc
    15721 gagctgaaag agctgatgcg tcagtctcag aaacccgtcg agcagcagcc ggtaatcact
    15781 caaccaccgc gaatgccaaa actggatgat gaggacatcg gtttcgatga agaaatctat
    15841 cagcaacgca tggctaagtg ggcagaggat aacggaaagt accagcaaca ggagatggct
    15901 cgcaagcaga aggagcagga gcttcaggct gcttatcaag agcgattatc caaatatcag
    15961 caacgtgtta aggctctcaa agttcctggc tatcaggaag ctgagcaggc cgtactcgag
    16021 gaaatcccca tcgagacaca aaacgcgatc ttgtttgagt cagagaagcc ggaaatcgtt
    16081 gttctggcac tcggtcgcaa cgctgaactg cgcaagcaac tggcagaagc taccaacccc
    16141 gtagcaattg gtcgtctgct ggaacgtatc gaatcgaagg ccagaatcat gccaaaagca
    16201 aaaaccacgg cagccacaac cccgacagtt aaggggagca acggcgcagt aatcaacaac
    16261 ctcgacaaac tgaaagccaa ggcgctggaa actggtgact ggacgccgta tttcgccgct
    16321 aaaaaggcaa aaaaataacc tatcggagca ttaagcatgg ctaaccaatt agcaaaagac
    16381 cttgaaatca tgttcgaaaa ctacgttgaa ggctttgagg ccgcctgcgt agtttcccgt
    16441 aacgctaaaa aattccgtcc cggtgataca gcaatgcagc gagcaggtga tgttctgtat
    16501 cgtccgcagc attaccacat gaacattgag gaaggcctag acctcagcgg caaaacgcca
    16561 acagcactgg ttcagcgcct tgttccttct gtgttcaagg agccgaaaaa cattctgtac
    16621 actctggatg cgcgtgaaat gcgtgacccg gaacataaaa ctgaagctgg tcgcgccgca
    16681 ggtatgcgcc ttgctgcaca gattgactct gacctgattt ccatggttac gcagcgtgct
    16741 actaacgtga tcacgatggc tgactcaacc acagggtcac agggccgtga tttgtggaac
    16801 tgtgcggcag gtattgatgc caccatgacg gcgattggtg tacctcaggg tatcaaccgt
    16861 cgctctttct ggaacccctt caactacaaa gaccttgctg gcgagcttgg tcaccgtgcc
    16921 tatgctcagg gcgcaaccct gacagcatac gaaaaagcgc aaatccctcc ggttgcgtcc
    16981 ttcgatagct acaagaccga tatttctggt cgtgttccga agggtacagc aacttccatt
    17041 acgctggcag cagcacctgc gcacaaggtt gaagcgaaag atgctaacga tatgccagtg
    17101 gataaccggc aggggaccat tacggtatct gctgaaggtt tgcaggttgg cgatgcgttt
    17161 accatcgcag gggtgaattc tgtacaccag atcaccaaag ataccaccgg gcagccgcag
    17221 gtattccgcg ttctggcagt aagcggaacg acagtaacta tctccccgaa aattctgccg
    17281 cctgacaacg cggatgtcgc cagccgacca tatgcaaacg ttgatgctaa tgcggcaagt
    17341 agcgcagcaa tcaccattct caacaaaaat gccgcaccgg ctaacctgtt ctgggctgat
    17401 ggttctgttg aactgatgta cggcaagctg gcgttcccaa ctggtcaggg tccacaagta
    17461 atgacagcaa ccaccgagca gggcgctacg ctgatcatgt cttacgcctt cgaccacatc
    17521 aaaggtgtaa ccactgcgcg tttcaccacc ctgtacggtt gctctgtact tgttcctgaa
    17581 tatacgggca tcgttattgc cgggcagtaa ttttggtggg gcttcggccc catttttatt
    17641 gggagaagac aatggcacga acaatgctct ataagccggg caacatgatc acctgtggtc
    17701 agtttgctgt cgattacatc attgttgatg acgaagaagt taaatctcac ctgaaaaaag
    17761 gctgggtaaa aactcctgaa gaaaccgcaa cgaagcaaaa agtggctaag gcggaagaag
    17821 atggcgaaaa cgaagggtga tctcgttctt aaggctttac gaaaagccgg gctgtattcc
    17881 aatgccacgt tgacagatgc cgaccctcag gcaattgaag atgccattaa tgacctcgaa
    17941 gacatgatgg cagcatggca ggcgaaaggt atcgagcttg gatatcagtt tgcggataca
    18001 gaaaacggca tcatgccgtt acctgacgat gattcaggta tccctgcatg ggcaaatgat
    18061 ggcgtcgctt tgaagctcgc tgtgcaagtg tgcatggata acgtcattca gccgtcagac
    18121 gctctcctta ccgctgctga cagtgcatat cagacaatct gcatcgcttt aaccaaaata
    18181 ccaccacttg agcggcgaaa tgacatgcct cgcggtagtg gtaacaaaag cgcgtttacg
    18241 tggaatcggt tttacatcga gaaagatgat ccgagtacgt gaggtgaata aatgccgatt
    18301 cagcaacttc cgctcatgaa aggtgtcggc aaagacttcc gaaatgccga ctatatcgac
    18361 tatctgccag tgaatatgct ggctacaccc aaagaaatcc tgaacagcag cggatatctt
    18421 cgctcattcc cgggcattgc caaacgctct gatgtgaacg gtgtatcgcg cggcgtcgag
    18481 tacaacatgg cgcagaatgc tgtttatcgt gtgtgtggtg ggaagctcta caaaggagaa
    18541 agtgaagtcg gtgatgtcgc cggaagtggt cgcgtatcaa tggcgcatgg tcgaacatca
    18601 caggctgtag gcgttaatgg tcaactggtc gagtatcgtt atgatggcac ggttaaaacc
    18661 gtctcaaact ggcctacaga cagtggattc acgcagtatg agttaggttc agttcgcgac
    18721 attactcgct tacgtgggcg ttatgcgtgg tcaaaagatg gtactgattc atggttcatc
    18781 actgaccttg aagacgaatc acatcctgac cgttacagcg cacaatatcg tgctgagtct
    18841 cagcctgacg gcatcatcgg aatcggaaca tggcgagact tcatcgtctg ctttggttca
    18901 tcgacgattg aatatttctc cctgactggc gcaaccaccg ttggtgctgc tttgtatgtc
    18961 gcacagccat cactgatggt gcaaaaaggc atcgccggga cttactgcaa aacaccattt
    19021 gctgattctt atgcgttcat cagcaaccct gcaacaggtg cgccgtctgt gtatatcatc
    19081 ggctctggtc aggtatcacc aatcgccagc gcgagcattg agaaaatact acgctcctac
    19141 actgctgatg aactggctga tggcgtgatg gaatcgttgc ggtttgatgc tcatgagttg
    19201 ctgattattc acctgccgcg ccatgttctt gtttacgacg catcttcaag cgccaatggt
    19261 ccgcaatggt gtgtgctgaa aacaggcttg tatgacgatg tgtaccgcgc tatcgacttc
    19321 atttacgaag gaaatcagat aacgtgcggc gataagctgg aatcggtgac cgggaaattg
    19381 cagttcgata tcagcagtca gtatgggctt caacaggaac acctgctgtt tactccactg
    19441 ttcaaagcgg ataacgccag atgcttcgat ctggaggtgg aatcatccac tggcgtagct
    19501 cagtacgccg accgcctgtt cctctctgca accactgacg gcataaatta tgggcgtgag
    19561 cagatgattg agcagaatga accgttcgtt tacgacaaac gcgttttgtg gaaacgagta
    19621 gggcgcatca ggaaaaacat tggtttcaaa ttgcgcgtta tcactaagtc acctgtcact
    19681 ctgtctggcg ctcagataag gattgagtaa tggctgattc gaatctcaat gagccagtaa
    19741 tcatccaggc tacgcggctc gacacatcag ttcttccacg caatatcttc tcgcagtcgt
    19801 atctgcttta tgttatcgca cagggcactg atgttggtaa cgtggctaac aaggccaacg
    19861 aagccggaca gggcgcttat gacgcacaag tcaggaacga tgagcaggat gtgattctcg
    19921 ctgaccatga gcagcgaatt tctgctgcgg aagcaacgct tgttaatcat gaggagagaa
    19981 tcagccaggc agaatcaact cttcaggaac atgaaacgcg aatcgctcag aatgaaagcg
    20041 atattgcgtc gcttgatacc agagttcagt cgctggagtc gcaggtttca gaccatgaat
    20101 cgcgcatcga tgctctggag tatgccacta ctcgcaagaa gtcagaggtt gtttactctg
    20161 gcgtatctgt aaccatcccg acagcgccga ccaaccttgt tagcctactg aaaacgctca
    20221 tgccgtcatc cggcacgttg gcaccattct tcgacaccgt taacaacaag atggttgtgt
    20281 tcaacgagaa caaaaccttg ttcttcaagc tgtcgatcgt cgggacgtgg cccagcggaa
    20341 ccgccaacag gtcaatgcag ctaacctttt ccggctctgt tcctgacaca ctggtaagca
    20401 gtcgcaactc ggcgacaaca accgataaca ttctgttagc tacgttcttc agcgtggata
    20461 aagacggctt tcttgccaca aatggcagta cgttaaccat tcagtcaaat ggtgcggcgt
    20521 ttactgccac aaccatcaaa atcattgcgg agcagtgatg gaaataaagc tcatcgataa
    20581 tccggtgaag cttgcggaat tcctcaacaa cccggcaaac acgggaaata tcgtagacag
    20641 tggagataaa tactacatca agcctgatgc ggtatatctc ggcatctacg aaggattagt
    20701 gctggctgga gttcatgaag tgcgtaactt ctggcatagc gttgttgaat gccatgcggt
    20761 gtatgacccc ggattccgtg gagaatatgc actgcaaggg catcgattat tctgcaaatg
    20821 gcttctcgaa aactcaccat tccttaacag catcaccatg gttcctgaca ccaccaaata
    20881 cggacgggca attatccgtt tgcttggcgc tacccgtgtt ggtcaccttg atgatgctta
    20941 taccagcaat ggaaagcctg taggcatcac gatttatcag ttaccgcgct caaaatacga
    21001 ggagctaaag aatgttaatt ttccagattg ccaataagca cctcagcaaa gctgtgtact
    21061 gcaaaggcgg cagtgatagc ggagcaaaag agcaggcccg cgcaactgaa aagggcatcg
    21121 aactgcagcg tgaaatgtgg cagacgaaca tgcaaaacct tgcaccgttc acgccactcg
    21181 ctcagcagta cgtatcacag ctgcagaatc tttcctctct tcaggggcaa ggtcaggcgc
    21241 ttaaccagta ttacaactct cagcagtata aagaccttgc agggcaggcg cgttaccaga
    21301 gtctggcagc agcagaggca acgggtggat taggctctac agcaacagga aaccagttag
    21361 ctgcaatcgc acctacactc ggtcaaaact ggctgtcagg tcagatgaac aactacaaca
    21421 atctggcaaa tatcggcctt ggtgctctta caggtcaggc aaacgccgga cagaactacg
    21481 ctaacaacgt cagccaactg tatcaacagc aggcggcagc atctgcggct aatgcgaata
    21541 aaccatcagg atttcagagc gccttgggtg gagcggcagc aggtgcagct gcaggtactg
    21601 caatcatgcc tggttggggt acagcaattg gtgctggcgt cggtcttctt ggttcacttt
    21661 tttaatggag gtgtctcttg gctacatggc aacaggctgg taattcaggc gcgcttcttg
    21721 ccgggttagg cggcatgaac tccaacgctc caagagcaag tgatgcagac gctacgcttg
    21781 catacattcg acaaaataac gagatggagc gttcaggacg taataacgtt ggcttgcagg
    21841 ctttgcaggg catttcatct gtcatggata tgtataagca gatggatcag cagaagcggc
    21901 agcaagagtt tcagcaggct tatgctgatg catatacatc tggtgaccgc gatgcaatgc
    21961 gaaaactggc atcacagtat cctgagcagt ttgacgctgt aagaaacggc atgaaatttg
    22021 tcgatgaaga ccagcgttcc actgtcggca cactggcagc aagtgccaga ctcgcagctt
    22081 catccccaga agccatgatg tcatggttgc agaacaactc atctgagctt actcgtgccg
    22141 gagtagaccc tctggatgtg gctaaaatgt atcagcaaaa tccacaaggt ttcacagagt
    22201 ttgttgatca ccttggaatg gctgcacttg gtccagaaca atattttcag gcacaagaca
    22261 gaatcgttgg tcgtgagatt gaccgaggca gactggcaga gacaatccgc agcaatcagg
    22321 caggagaggc acttcaggcg agagggcagg atattagccg agcaaatgcg ttaacgtcag
    22381 catatgcacc aacagccgca atgcagaatt acaatcagta cgcgcaaatg ttaaaggcgg
    22441 atccagatgg tgcagcggca tttgcggcag cggcgggaat taatcccaat gctaagaaat
    22501 tacttaaggt tgaaaccaat cctgatggct cggtaactaa gtattacacc gatggcagcg
    22561 aggaagcagg aaaactaaac caacctatat ctggtgatgg cattaaacca attagcttgc
    22621 cacaagcgca aagcatcata gataaggcta atgagggttc caagaaggcg gcaggatttg
    22681 ctttgcgatt aaaagattca atggactcaa tgaatcagct tagtaaaagc attgacccta
    22741 agcgagttgc attaataaat cgctctcttg gtgatgggac tattgcaaat ttaagcctat
    22801 caccagcgga gcagcaatat atggtaaatg cgagagacgc cttgtatgca attttgcgcc
    22861 cagaaacagg tgcagcaatt actctgccag agatgcagga gtattccaaa atgtacctgc
    22921 ctcagcccgg tgattccaag gctgctactg aaacaaaaat gcgaaaaatg cagggccaat
    22981 ataactcatt acgtggtcag tctggtcgcg tttatgatgc tttggtggtt tcaagtgctg
    23041 caaatagtca acaacagagc aatagccaac aaccgacaaa tacccaacag cagcagagtc
    23101 aatccggatc atatacctca aaatcaggca ttcaatttac ggtggaatga tgaaagtaac
    23161 tgcaaacggt aagacattta ccttccctga tggtacgagc acggaagata ttggcaccgc
    23221 cattgatgag tattttgctg gtcaggctgt tcagcaacaa acagttaatc aggccaataa
    23281 tgaaccagca cgtgaagaac catcattgat gcaacaagct ggcgattggc tcactggtgg
    23341 tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt ctggtaaaca taccatttga
    23401 cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt caggggcttg gtggccccaa
    23461 ggttttggat gatgtttatc gtccagtaga cagaccgaca gacccctacg ctcaagctgg
    23521 agaaacaatt ggtgggtatt tagttccagg agttggaacg gcaggaagca tggctattgg
    23581 atcactggca gaggccgcaa atcagaaagg cgatttcgca caaaacgcag ctaaaaatgc
    23641 cggagttaac cttgccgctc agggtgttct ttccgcagca gcaaagggaa tagggcgtgg
    23701 aataacggct ataaaaggtg atattgcgcc agaagtggcg aagaaaattg ccacatcaga
    23761 atcgatgggc gtgacaccaa tgacatctga tgttatcccg ccgaaaaatg ctttcactcg
    23821 cggccttact caggatgccg agggggcttt gctcgggaca ggctcaaagc gagcggagca
    23881 atatgcaacg cgtagtaagt tggtaagtaa ttattttgac cgttttggtg agtacaaccc
    23941 ggatgatgtg gtgaaatctc tgaccatcac gttaaggggg cggaaggatg ctgctggcgc
    24001 tgttatcaat gacgtcacca ataaaatggg taatgccgca gttgatacca caaataccat
    24061 gaatgctctg aatgcagcga tcgcaagaca ggaacgactt gggacttcag ccaatcaaag
    24121 cctgcttaca tccttgcgta acctacgtga agaattagct aaccctgcaa ctgatttgga
    24181 tgttacgttt gatctcttgc gtcagcacag aacagcattt agatctaatg ttcagggaga
    24241 tgctatggtc ttccccaacc aggcaaaagc agctaccaat atggtagaga atgcaatgtc
    24301 aaaagacctt cgtaacgcag ttgcaaaaaa ccttggtgcg tcagacgcag caaaatacct
    24361 taaagcaaat tccgattatg caaacgttta taataaggtg cttaataaaa acattgctaa
    24421 caaactcaac aaggcaagca gtgaagccag tcctgaactt ataaataccg ttgtattaag
    24481 cagaaaacca tctgacgtga aacgaatctg gagcgcactg gatgataaag ggaaagatgc
    24541 tatgcgtgca gcttacgtca gcaaaatagc ggaaaaggcc ggtgactctc cagccaagtt
    24601 catcactgaa gttaataagc tgaaatctca gtcaggcggt gaaatttaca acactatttt
    24661 ttctggaaag cacatgaaag agcttgatgc tcttcatgaa gttctacagc aaacagcaag
    24721 gtcagacacc gcaaatgtag taactcagac ggggcaatca caagccaaca ggataaggac
    24781 gattggcgca actgcgactc ttggagtatc aatggggctt gaggctggtt ttggtgcaat
    24841 gatgcgtttg tatgagtcca aagcagcaag gaatgcgctc ttacgtctgg caaacactaa
    24901 agctggaacg ccagcttatg aaagagcgct aaatcaggct gctactgccg tgcgcccgct
    24961 cttagctaac gaagcgaccc ggcagt
//
LOCUS       DAZGAP010000071        24857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGAP010000071 DAZGAP010000000
VERSION     DAZGAP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.071"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..628
                     /gene="nohD"
                     /locus_tag="ACHLAM_002390"
     CDS             83..628
                     /gene="nohD"
                     /locus_tag="ACHLAM_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO7853906.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            603..2528
                     /locus_tag="ACHLAM_002391"
     CDS             603..2528
                     /locus_tag="ACHLAM_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7853907.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2525..2731
                     /locus_tag="ACHLAM_002392"
     CDS             2525..2731
                     /locus_tag="ACHLAM_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO7853908.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2728..4329
                     /locus_tag="ACHLAM_002393"
     CDS             2728..4329
                     /locus_tag="ACHLAM_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7853909.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4310..5629
                     /locus_tag="ACHLAM_002394"
     CDS             4310..5629
                     /locus_tag="ACHLAM_002394"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7853910.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5639..5971
                     /locus_tag="ACHLAM_002395"
     CDS             5639..5971
                     /locus_tag="ACHLAM_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7853911.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6027..7052
                     /locus_tag="ACHLAM_002396"
     CDS             6027..7052
                     /locus_tag="ACHLAM_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7853912.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            7094..7489
                     /locus_tag="ACHLAM_002397"
     CDS             7094..7489
                     /locus_tag="ACHLAM_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7853913.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            7501..7854
                     /locus_tag="ACHLAM_002398"
     CDS             7501..7854
                     /locus_tag="ACHLAM_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7853914.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7866..8444
                     /locus_tag="ACHLAM_002399"
     CDS             7866..8444
                     /locus_tag="ACHLAM_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7853915.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRISGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8441..8836
                     /locus_tag="ACHLAM_002400"
     CDS             8441..8836
                     /locus_tag="ACHLAM_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7853916.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8844..9584
                     /locus_tag="ACHLAM_002401"
     CDS             8844..9584
                     /locus_tag="ACHLAM_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7853917.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGAIDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            9600..10022
                     /locus_tag="ACHLAM_002402"
     CDS             9600..10022
                     /locus_tag="ACHLAM_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7853918.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            10004..10438
                     /locus_tag="ACHLAM_002403"
     CDS             10004..10438
                     /locus_tag="ACHLAM_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7853919.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10431..13010
                     /locus_tag="ACHLAM_002404"
     CDS             10431..13010
                     /locus_tag="ACHLAM_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7853920.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            13007..13336
                     /locus_tag="ACHLAM_002405"
     CDS             13007..13336
                     /locus_tag="ACHLAM_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7853921.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13336..14034
                     /locus_tag="ACHLAM_002406"
     CDS             13336..14034
                     /locus_tag="ACHLAM_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7853922.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14040..14783
                     /locus_tag="ACHLAM_002407"
     CDS             14040..14783
                     /locus_tag="ACHLAM_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7853923.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14780..15352
                     /locus_tag="ACHLAM_002408"
     CDS             14780..15352
                     /locus_tag="ACHLAM_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7853924.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15412..18909
                     /locus_tag="ACHLAM_002409"
     CDS             15412..18909
                     /locus_tag="ACHLAM_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7853925.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            18980..19579
                     /locus_tag="ACHLAM_002410"
     CDS             18980..19579
                     /locus_tag="ACHLAM_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFO7853926.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19644..22670
                     /locus_tag="ACHLAM_002411"
     CDS             19644..22670
                     /locus_tag="ACHLAM_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7853927.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            22667..23569
                     /locus_tag="ACHLAM_002412"
     CDS             22667..23569
                     /locus_tag="ACHLAM_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFO7853928.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            23578..24153
                     /locus_tag="ACHLAM_002413"
     CDS             23578..24153
                     /locus_tag="ACHLAM_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7853929.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(24251..24841)
                     /locus_tag="ACHLAM_002414"
     CDS             complement(24251..24841)
                     /locus_tag="ACHLAM_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7853930.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         5888 a         6276 c         7575 g         5118 t
ORIGIN      
        1 aagaaaggaa acgacaggtg ctgaaagcga gctttttggc ctctgtcgtt tcctttctct
       61 gtttttgtcc gtggaatgaa caatggaagt caacaaaaag cagctggctg acattttcgg
      121 tgcgagtatc cgtaccattc agaactggca ggaacaggga atgcccgttc tgcgaggcgg
      181 tggcaagggt aatgaggtgc tttatgactc tgccgccgtc ataaaatggt atgccgaaag
      241 ggatgctgaa attgagaacg aaaagctgcg ccgggaagtt gaagaactgc ggcaggccag
      301 cgagacagat ctccagccag ggactattga gtacgaacgc catcgactta cgcgtgcgca
      361 ggccgacgca caggagctga aaaatgccag agactccgct gaagtggtgg aaaccgcatt
      421 ctgtactttc gtgctgtcgc ggatcgcagg tgaaattgcc agtattctcg acgggatccc
      481 cctgtcggtg cagcggcgtt ttccggaact ggaaaaccga catgttgatt tcctgaaacg
      541 ggatatcatc aaagccatga acaaagcagc cgcgctggat gaactgatac cggggttgct
      601 gagtgaatat atcgaacagt caggttaaca ggctgcggca ttttgtccgc gccgggcttc
      661 gctcactgtt caggccggag ccacagaccg ccgttgaatg ggcggatgcc aattactatc
      721 tcccaaaaga atccgcatac caggaagggc gctgggaaac actgcccttt cagcgggcca
      781 tcatgaatgc gatgggcagc gactacatcc gtgaggtgaa tgtggtgaag tctgcccgtg
      841 tcggttattc caaaatgctg ctgggtgttt atgcctactt tatagagcat aagcagcgca
      901 acacccttat ctggttgccg acggatggtg atgccgagaa ctttatgaaa acccacgttg
      961 agccgaccat ccgcgatatt ccgtcgctgc tggcgctggc tccgtggtat ggcaaaaagc
     1021 accgggataa cacgctcact atgaagcgtt tttccaatgg tcgtggcttc tggtgcctgg
     1081 gcggtaaagc ggcaaaaaac taccgtgaaa agtcggtgga tgtggcgggt tatgatgaac
     1141 ttgctgcctt tgatgaggat attgaacagg aaggctctcc gacgttcctt ggcgacaaac
     1201 gtattgaagg ctcggtctgg ccaaagtcca tccgtggctc cacgcccaaa gtgagaggca
     1261 cctgccagat tgagcgtgca gccagtgaat ccccgcattt tatgcgtttt catgttgcct
     1321 gcccgcactg cggggaggag cagtatctta aatttggcga caaagagacg ccgtttggcc
     1381 tcaaatggac gccggatgac ccctccagcg tgttttatct ctgcgagcat aatgcctgcg
     1441 tcatccgcca gcaggagctg gactttactg atgcccgtta tatctgcgaa aagaccggga
     1501 tctggacccg tgatggcatt ctctggtttt cgtcatccgg tgaagagatt gagccgccgg
     1561 acagtgtgac ctttcacatc tggacggcgt acagcccgtt caccacctgg gtgcagattg
     1621 tcaaagactg gatgaagacg aaaggggata cgggaaaacg taaaaccttc gtgaacacca
     1681 cgctcggtga gacatgggaa gcgaaaattg gcgaacgtcc ggatgctgag gtgatggcgg
     1741 agcggaaaga gcattattca gcgcccgttc ctgaccgtgt ggcttacctg accgccggta
     1801 tcgactccca gctggaccgc tacgaaatgc gcgtatgggg atgggggccg ggtgaggaaa
     1861 gctggctgat tgaccggcag attattatgg gccgccacga cgatgaacag acgctgctgc
     1921 gtgtggatga ggccatcaat aaaacctata cccgccggaa tggtgcagaa atgtcggtat
     1981 cccgtatctg ctgggatact ggcgggattg acccgaccat tgtgtatgaa cgctcgaaaa
     2041 agcatgggct gttccgggtg atccccatta aaggggcatc cgtctacgga aagccagtgg
     2101 ccagcatgcc acgtaagcga aacaaaaacg gggtttacct taccgaaatc ggtacggata
     2161 ccgcgaaaga gcagatttat aaccgcttca cactgacgcc ggaaggggat gaaccgcttc
     2221 ccggtgccgt tcacttcccg aataacccgg atatttttga tctgaccgaa gcgcagcagc
     2281 tgactgctga agagcaggtc gaaaaatggg tggatggcag gaaaaaaata ctgtgggaca
     2341 gcaaaaagcg acgcaatgag gcgctcgact gcttcgttta tgcgctggcg gcgctgcgca
     2401 tcagtatttc ccgctggcag ctggatctca gtgcactgct ggcgagcctg caggaagagg
     2461 atggtgcagc aaccaacaag aaaacactgg cagattacgc ccgtgcctta tccggagagg
     2521 atgaatgacg cgacaggaag aacttgccgc tgcccgtgcg gcactgcatg acctgatgac
     2581 aggaaaacgg gtggcaacgg tacagaaaga cggacggcga gtggagttta cggccacttc
     2641 cgtgtctgac ctgaaaaaat acattgcgga gctggaagtg cagaccggca tgacacagcg
     2701 acgcagggga cctgcaggat tttatgtatg aaaacgtcct ccattcccac ccttctgggg
     2761 ccggacggca tgacatcgct gcgtgaatat gccggttatc acggcggtgg cagcggattt
     2821 ggtgggcagt tgcgggcgtg gaacccaccg agtgaaagtg tggatgcagc cctgttgccc
     2881 aactttaccc gtggcaatgc ccgcgcggac gatctggtac gcaataacgg ctatgccgcc
     2941 aacgccatcc agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc
     3001 ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt
     3061 gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa
     3121 cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg
     3181 ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg
     3241 gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc
     3301 ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat
     3361 cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgtgcc
     3421 tcgttcattc acgtttttga acccgtggag gacgggcaga cccgcggtgc aaatgtgttt
     3481 tacagcgtga tggagcagat gaagatgctc gacacgctgc agaacacgca gctgcagagc
     3541 gccattgtga aggcgatgta tgccgccacc attgagagtg agctggatac gcagtcagcg
     3601 atggatttta ttctgggcgc gaacagtcag gagcagcggg aaaggctgac cggctggatt
     3661 ggtgaaattg ccgcgtatta cgccgcagca ccggtccgtc tgggaggcgc aaaagtgccg
     3721 cacctgatgc cgggggactc actgaacctg cagacggctc aggacacgga taacggctac
     3781 tccgtgtttg aacagtcact gttgcggtat atcgctgccg ggctgggtgt ctcgtatgag
     3841 cagctttccc ggaattacgc ccagatgagc tactccacgg cacgggccag tgcgaacgag
     3901 tcgtgggcgt actttatggg gcggcgaaaa ttcgtcgcat cccgtcaggc gagccagatg
     3961 tttctgtgct ggctggaaga ggccatcgtt cgccgcgtgg tgacgttacc ttcaaaagcg
     4021 cgtttcagct ttcaggaagc ccgcagcgcc tgggggaact gtgactggat aggctccggt
     4081 cgtatggcca tcgatggtct gaaagaagta caggaagcgg tgatgctgat agaagccgga
     4141 ctgagtacct acgagaaaga gtgcgcaaaa cgcggtgacg actatcagga aatttttgcc
     4201 cagcaggtcc gtgaaacgat ggagcgccgt gcagccggtc ttaaaccgcc cgcctgggcg
     4261 gctgcggcat ttgaatccgg actgcgacaa tcaacagagg aggagaagag tgacagcaga
     4321 gctgcgtaat ctcccgcata ttgccagcat ggcttttaat gagccgctga tgcttgaacc
     4381 cgcctatgcg cgggttttct tttgtgcgct tgcaggccag cttgggatca gtcgcctgac
     4441 ggatgcagta tccggcgaca gcctgactgc cggagaggca cccgcggcgc tggcgttatc
     4501 cggtgatgat gacggaccac gacaggcccg cagttatcag gtcatgaacg gcatcgccgt
     4561 gctgccggtg tccggtacgc tggtcagccg gacgcgggcg ctgcagccgt attcgggaat
     4621 gaccggttac aacggcatta tcgcccgtct gcaacaggct gccagcgatc cgatggtgga
     4681 cggcattctg ctcgatatgg acacaccggg cgggatggtg gcgggagcat ttgactgtgc
     4741 tgacatcatc gcccgtgtgc gtgacataaa gccggtatgg gcgctggcca acgacatgaa
     4801 ctgcagtgca ggtcagctgc ttgccagcgc cgcctcccgg cgtctggtca cgcagaccgc
     4861 ccggacaggc tccatcggcg tcatgatggc tcacagtaat tacggcgctg cgctggagaa
     4921 acagggcgtg gaaatcacgc tgatttacag cggcagccat aaggtggatg gcaaccccta
     4981 cagccatcta ccgggtgatg tccgggagac actgcagtcc cggatggatg caacccgccg
     5041 gatgtttgcg cagaaggtgt cggcatatac cggcctgtcc gtgcaggctg tgctggatac
     5101 cgaggctgca gtgtacagcg gtcaggaggc cattgatgcc ggactggctg atgaacttgt
     5161 caacagcacc gatgcgatca ccgttatgcg tgatgcactg gatgcacgta aatcccgtct
     5221 ctcaggaggg cgaatgacca aagagactca atcaacaact gtttcagcca ctgcttcgca
     5281 ggctgacgtt actgacgtgg tgcaagcgac ggagggcgaa aacgccagcg cggcgcagcc
     5341 ggacgtgaac gcgcagatca ccgctgcggt tgcggcagaa aacagccgca ttatggggat
     5401 cctcaactgt gaggaagctc acggacgcga agaacaggca cgtgtgctgg ccgaaacccc
     5461 cggtatgacc gtggaaacgg cccgccgcat tctggcagct gcaccacaga gtgcacaggc
     5521 gcgcagtgac actgcgctgg atcgtctgat gcagggggca ccggcaccac tggctgcagg
     5581 taacccggca tctgatgccg ttaacgattt gctgaacaca ccagtgtaag ggatgtttat
     5641 gacgagcaaa gaaaccttta cccattacca gccgctgggc aacagtgacc cggcacatac
     5701 ggcaaccgcg cccggcggat tgagtgcgaa agcgcctgca atgaccccgc tgatgctgga
     5761 cacctccacc cgtaagctgg ttgcgtggga tggcaccacc gacggtactg ccgttggcat
     5821 tctggcggtt gatgctgacc agaccagcac cacgctgacg ttctacaagt ccggcacgtt
     5881 ccgttatgag gatgtgctct ggccggaggc tgccagcgac gagacgaaaa aacggaccgc
     5941 gtttgccgga acggcaatca gcatcgttta accttaccct tcatcactaa aggccgcctg
     6001 tgcggctttt tttacgggat ttttttatgt cgatgtacac aaccgcccag ctgctggcgg
     6061 caaatgagca gaaatttaag tttgatccgc tgtttctgcg tctctttttc cgtgagagct
     6121 atcccttcac cacggagaaa gtctatctct cacaaattcc gggactggta aacatggcgc
     6181 tgtacgtttc gccgattgtt tccggtgagg ttatccgttc ccgtggcggc tccacctctg
     6241 aatttacgcc gggatatgtc aaacccaagc atgaggtgaa tccgcagatg accctgcgtc
     6301 gcctgccgga tgaagatccg cagaatctgg cggacccggc ttaccgccgc cgtcgcatca
     6361 tcatgcagaa catgcgagac gaagagctgg ccattgctca ggtcgaagag atgcaggcag
     6421 tttctgctgt gcttaagggc aaatacacca tgaccggtga agccttcgat ccggttgagg
     6481 tggatatggg ccgcagtgcg gcgaacaaca tcacacagtc cggtggtacg gagtggagca
     6541 agcgtgacaa gtccacgtat gacccgaccg acgatatcga agcctatgcg ctgaacgcca
     6601 gcggcgtggt gaatatcatc gtgtttgatc cgaaaggctg ggcgctgttc cgttccttca
     6661 aagccgtcaa ggagaagctg gatacccgtc gcggctctca ttccgagctg gagacagcgg
     6721 taaaagacct gggcgaagcg gtgtcctata aggggatgta tggcgatacg gcgatcgtcg
     6781 tgtattccgg acagtacgtg gaaaacgacg tcaaaaagaa cttcctgccg gacaacacga
     6841 tggtgctggg gaacactcag gcacgcggtc tgcgcaccta tggctgcatt caggatgcgg
     6901 acgcacagcg cgaaggtatt aacgcctctg cccgctaccc gaaaaactgg gtgaccaccg
     6961 gcgatccggc gcgtgagttc accatgattc agtcagcacc gctgatgctg ctggctgatc
     7021 ctgatgcgtt cgtgtccgta caactggcgt aatcatggcc cttcggggcc attttctctc
     7081 tgtggaggag tccatgacga aagatgaact gattgcccgt cttcaggtgc tgggtgagca
     7141 actgaaccgt gatgtcagcc tgacggggac gaaagaagaa ctggtgctcc gtgtggcaga
     7201 gctggaagag gagcttgatg acacggatga cgctgccggt caggacacat ctgtcagccc
     7261 ggaaaatgcg ctgaccggac atgaaaatga ggtggtatca gcgcagccgg ataccgtgat
     7321 tgatacggct gctctggtca cggtcgtggc actggtgacg ctgcatactg atgcacttca
     7381 cgccacgcgg gatgagcctg tggcatttgt gctgccggga acggcgtttc gtgtctctgc
     7441 cggtgtggca gccgaaatga cagaacatgg cctggccaga atgcaataac gggaggcgct
     7501 gtggctgatt ccgataacct gttcgatgct gccattgccc gcgccgatga aacgatacgc
     7561 gggtacatgg gaacgtcagc caccatgaca tccggtgagc tgtccggtgc tgtgatacgt
     7621 ggtgtttttg atgaccctga aaatatcagc tatgccggac agggggtgcg cgttgaaggc
     7681 tccagcccgt ccctgtttgt ccggactgat gatgtgcggc agctgcggcg tggagacaca
     7741 ctgaccatcg gcgaggaaaa cttctgggtg gaccggattt cgccggatga tggcggaagc
     7801 tgtcatctct ggcttgggcg tggcgtgccg cctgccgtta accgtcgccg ctgaaagggg
     7861 gatgtatggc cataaaaggt cttgagcagg ccgttgaaaa cctcagccgt atcagcaaaa
     7921 cggcggtgcc tggtgccgcc gcaatggcca ttaaccgcgt tgcgtcatcc gcgatatcgc
     7981 agtctgcgtc acaggttgcc cgtgagacaa aggtacgccg gaaactggta aaggaaaggg
     8041 ccaggctgaa aagggccacg gtcaaaaatc cgcaggccag aatcagggtt aaccgggggg
     8101 atttgcccgt aatcaagctg ggtaacgcgc gggttgtcct ttcccgccgc agacgtcgta
     8161 aaaaggggca gcgttcatcc ctgaaaggtg gcggcagcgt gcttgtggtg ggaaaccgtc
     8221 gtatttccgg cgcgtttatt cagcaactga aaaatggccg gtggcatgtc atgcagcgtg
     8281 tggccgggaa aaaccgttac cccattgatg tggtgaaaat cccgatggcg gtgccgctga
     8341 ccacggcgtt taaacagaat attgaacgga tacggcgtga acgtcttccg aaagagctgg
     8401 gctatgcgct gcagcatcaa ctgagaatgg taataaagcg atgaaacata ctgaactccg
     8461 tgcagccgta ctggatgcac tggagaagca tgacaccggg gcgacgtttt ttgatggtcg
     8521 ccccgctgtt tttgatgagg cggattttcc ggcagttgcc gtttatctca ccggcgctga
     8581 atacacgggc gaagagctgg acagcgatac atggcaggcg gagctgcata ttgaagtttt
     8641 cctgcctgct caggtgccgg attcagagct ggatgcgtgg atggagtccc ggatttatcc
     8701 ggtgatgagt gatatcccgg cactgtcaga tttgatcacc agtatggtgg ccagcggcta
     8761 tgactaccgg cgcgacgatg atgcgggcct gtggagttca gccgatctga cttatgtcat
     8821 tacctatgaa atgtgaggac gctatgcctg taccaaatcc tgtaatgccg gtgaaaggtg
     8881 ccgggaccac cctgtgggtt tataagggaa gcggtgaccc ttatgcgaat ccgctttcag
     8941 acgttgactg gtcgcgtctg gcaaaagtta aagacctgac gcccggcgaa ctgaccgctg
     9001 agtcctatga cgacagctat ctcgatgatg aagatgcaga ctggactgcg accgggcagg
     9061 ggcagaaatc tgccggagat accagcttca cgctggcgtg gatgcccgga gagcaggggc
     9121 agcaggcgct gctggcgtgg tttaatgaag gggatacccg agcctataaa atccgcttcc
     9181 cgaacggcac ggtcgatgtg ttccgcggct gggtcagcag tatcggtaag gcggtgacgg
     9241 cgaaggaagt gatcacccgc acggtgaaag tcaccaacgt gggccgtccg tcgatggcag
     9301 aagatcgcag cacggtgacg gcgacaaccg gcatgactgt gacgcctgcc agcacctcgg
     9361 tggtgaaagg gcagagcacc acgctgaccg tggcattcca gccggaaggc gcaatcgaca
     9421 agagcttccg tgcggtgtct gcggataaaa caaaagccac cgtgtcggtc agtggtatga
     9481 ccatcaccgt gaaaggtgtt gctgcaggca aggtcaacat tccggtcgta tccggtaatg
     9541 gtgaacttgc tgcggttgca gaaatcaccg tcaccgccag ttaatccggg gagtcagcga
     9601 tgttcctgaa aaccgaatca tttgaacata acggtgtgac cgtcacgctt tctgaactgt
     9661 cagccctgca gcgaattgag catctcgccc tgatgaaacg gcaggcagaa caggcggagt
     9721 cagacagcaa ccggaagttt actgtggaag acgccatcag aaccggcgcg tttctggtgg
     9781 cgatgtccct gtggcataac catccgcaga agacgcagat gccgtccatg aatgaagccg
     9841 ttaaacagat tgagcaggaa gtgcttacca cctggcccac ggaggcaatt tctcatgctg
     9901 aaaacgtggt gtaccggctg tctggtatgt atgagtttgt tgtgaataat gcccctgaac
     9961 agacagagga cgccgggccc gcagagcctg tttctgcggg aaagtgttcg acggtgagct
    10021 gagttttgcc ctgaaactgg cgcgagagat ggggcgaccc gactggcgtg ccatgcttgc
    10081 cgggatgtca tccacggagt atgccgactg gcaccgcttt tacagtaccc attattttca
    10141 tgatgttctg ctggatatgc acttttccgg gctgacgtac accgtgctca gcctgttttt
    10201 cagcgatccg gatatgcatc cgctggattt cagtctgctg aaccggcgtg aggctgacga
    10261 agagcctgaa gatgatgtgc tgatgcagaa agcggcaggg cttgccggag gcgtccgctt
    10321 tggcccggac gggaatgaag ttatccccgc ttccccggat gtggcggaca tgacggagga
    10381 tgacgtaatg ctgatgacag tatcagaagg gatcgcagga ggagtccggt atggctgaac
    10441 cggtaggcga tctggtcgtt gatttaagtc tggatgcggc cagatttgac gagcagatgg
    10501 ccagagtcag gcgtcatttt tccggtacgg aaactgatgc gaaaaaaaca gcggcagtcg
    10561 ttgaacagtc gctgagccga caggcgctgg ctgcacagaa agcggggatt tccgtcgggc
    10621 agtataaagc cgccatgcgt atgctgcctg cacagttcac cgacgtggcc acgcagcttg
    10681 caggcgggca aagcccgtgg ctgatcctgc tgcaacaggg gggtcaggtt aaggactcct
    10741 tcggcgggat gatccccatg ttcagggggc ttgccggtgc gatcaccctg ccgatggtgg
    10801 gggccacctc gctggcggtg gcgaccggtg cgctggcgta tgcctggtat cagggcaact
    10861 caaccctgtc cgatttcaac aaaacgctgg tcctttccgg caatcaggcg ggactgacgg
    10921 cagatcgtat gctggtcctg tccagagccg ggcaggcggc agggctgacg tttaaccaga
    10981 ccagcgagtc actgacggcg ctggtgaatg ccggtgtgcg tggtggtgag cagtttgagg
    11041 cgatcagcca gagtgtggcg cgtttctcct ctgcatccgg cgtggaggtg gacaaggtcg
    11101 ctgaagcctt cgggaagctg accacagacc cgacgtcggg actgacagcg atggcacgtc
    11161 agttccataa cgtgacggcg gagcagattg cgtatgttgc tcagttgcag cgttccggag
    11221 atgaagccgg ggcattgcag gcggcgaacg aggccgcaac gaaagggttt gatgaccaga
    11281 cccgccgcct gaaagagaac atgggcacgc tggagacctg ggcagacagg acagcacggg
    11341 cattcaaatc catgtgggat tcggtgctgg atattggtcg cccggacact gcccagggaa
    11401 tgctggagaa agcagaaaag gcttttgatg aggcggacaa aaaatggcag tggtatcaga
    11461 gccggagcca ccggcgcggt aaaacctcag catttcttgc caatctccgg ggagcatggg
    11521 aggacagagc gaatgcgcaa cttgggcttt cagccgccac gttgcaggcc gatcttgaaa
    11581 aggccagaga gatggcagca aaggactggg ccgagtctga ggcatcacgg ctgaaatata
    11641 ccgaagaggc gcagaaggct tacgaacggc tgcagacgcc gctggagaaa tataccgccc
    11701 gtcaggaaga actgaacaag gcactgaaag acgggaaaat cctgcaggcg gattacaaca
    11761 cgctgatggc ggcggcgaaa aaggattatg aagcgacgct gaaaaagccg aaacagtccg
    11821 gcgtgaaggt gtctgcgggc gatcgtcagg aagacagtgc tcatgctgcc ctgctgacgc
    11881 ttcaggctga actccggacg ctggagaagc atgccggagc aaatgagaaa atcagccagc
    11941 agcgccggga tttgtggaag gcggagagcc agttcgcggt actggaggag gcggcgcaac
    12001 gtcgccagct gtctgcacag gagaaatccc tgctggcgca taaagatgag acgctggagt
    12061 acaaacgcca gctggctgca cttggcgaca aggttacgta tcaggagcgc ctgaacgcgc
    12121 tggcgcagca ggcggataaa ttcgcacagc agcaacgggc aaaacgggcc gccattgatg
    12181 cgaaaagccg ggggctgact gaccggcagg cagaacggga agccacggaa cagcgcctga
    12241 aggaacagta tggcgataat ccgctggcgc tgaataacgt catgtcagag cagaaaaaga
    12301 cctgggcggc tgaagaccag cttcgcggga gctggatggc aggcctgaag tccggctgga
    12361 gtgagtggga agagagcgcc acggacagta tgtcgcaggt aaaaagtgca gccacgcaga
    12421 cctttgatgg tattgcacag aatatggcgg cgatgctgac cggcagtgag cagaactggc
    12481 gcagcttcac ccgttccgtg ctgtccatga tgacagaaat tctgcttaag caggcaatgg
    12541 tggggattgt cgggagtatc ggcagcgcca ttggcggggc tgttggtggc ggcgcatccg
    12601 cgtcaggcgg tacagccatt caggccgctg cggcgaaatt ccattttgca accggaggat
    12661 ttacgggaac cggcggcaaa tatgagccag cggggattgt tcaccgtggt gaatttgtct
    12721 tcacgaagga ggcaaccagc cggattggcg tggggaatct ttaccggctg atgcgcggct
    12781 atgccaccgg cggttatgtc ggtacaccgg gcagcatggc agacagccgg tcgcaggcgt
    12841 ccgggacgtt tgagcagaat aaccatgtgg tgattaacaa cgacggcacg aacggtcaga
    12901 taggtccggc tgctctgaag gcggtgtatg acatggcccg caagggtgcc cgtgatgaaa
    12961 ttcagacaca gatgcgtgat ggtggcctgt tctccggagg tggacgatga agaccttccg
    13021 ctggaaagtg aaacccggta tggatgtggc ttcggtccct tctgtaagaa aggtgcgctt
    13081 tggtgatggc tattctcagc gagcgcctgc cgggctgaat gccaacctga aaacgtacag
    13141 cgtgacgctt tctgtccccc gtgaggaggc cacggtactg gagtcgtttc tggaagagca
    13201 cgggggctgg aaatcctttc tgtggacgcc gccttatgag tggcggcaga taaaggtgac
    13261 ctgcgcaaaa tggtcgtcgc gggtcagtat gttgcgtgtt gagttcagcg cagagtttga
    13321 acaggtggtg aactgatgca ggatatccgg caggaaacac tgaatgaatg cacccgtgcg
    13381 gagcagtcgg ccagcgtggt gctctgggaa atcgacctga cagaggtcgg tggagaacgt
    13441 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg tcacctggca ggggcgacag
    13501 tatcagccgt atcccattca ggggagcggt tttgaactga atggcaaagg caccagtacg
    13561 cgccccacgc tgacggtttc taacctgtac ggtatggtca ccgggatggc ggaagatatg
    13621 cagagtctgg tcggcggaac ggtggtccgg cgtaaggttt acgcccgttt tctggatgcg
    13681 gtgaacttcg tcaacggaaa cagtgacgcc gatccggagc aggaggtgat cagccgctgg
    13741 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtact gtccacgccg
    13801 acggaaacgg acggtgccgt ttttccggga cgtatcatgc tggccaacac ctgcacctgg
    13861 acctatcgcg gtgatgagtg cggttatcac ggtccggcgg tcgcggatga atatgatcag
    13921 ccgacgtccg atatcacgaa ggataaatgc agcaaatgcc tgagcggctg taagtttcgc
    13981 aataacgtcg gcaactttgg cggcttcctt tccattaaca aactttcgca gtgaatccca
    14041 tgacagagac agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt
    14101 gcggcttcgt ggtgagaaca ccggaggggg aaagatattt tccctgcgtg aatatctccg
    14161 gtgagccgga ggcgtatttc cggatgtcgc cggaggactg gctgcgggca gagatgcagg
    14221 gtgagattgt ggcgctggtc cacagccacc ccggtggtct gccctggctg agtgagaccg
    14281 accggcggct gcaggtgcag agtgatttgc cgtggtggct ggtttgccgg ggggcgattc
    14341 acaagttccg ctgtgtgcca catctttccg ggcggcgctt tgagcacggg gtgacggact
    14401 gttacacgct gttccgggat gcttaccatc tggcggggat tgagatgccg gattttcatc
    14461 gcgaggatga ctggtggcgt cacggtcaga atctctatct ggataatctg gaggccacag
    14521 ggctgtatca ggtgccgttg tcatcagcac aaccgggcga tgtgctgctg tgctgttttg
    14581 gttcatcggt gccgaatcat gccgccattt actgtggtga cagcgagctg ctgcaccata
    14641 ttcctgaaca actgagcaaa cgagagaggt acaccgacaa atggcagcga cgcacacact
    14701 ccctctggcg tcaccgggaa tggcacgcat ctgcctttac ggggatttgc aacgatttgg
    14761 ccgccgcatc gaccttcgtg tgaaaacggg ggctgaagcc atccgcgcac tggccacaca
    14821 gctcccggcg tttcgtcaga aactgagcga cggctggtat caggtacgga ttgccgggcg
    14881 ggacgtcagc acgtccgggt taacggcgca gttacatgag actctgcctg acggcgctgt
    14941 gattcatatt gttcccagag tcgccggggc caagtcaggg ggcgtattcc agattgttct
    15001 gggggctgcc gccattgccg gatcattctt tactgccgga gccacccttg cagcatgggg
    15061 ggcagccatt ggggccggtg gtatgaccgg catcctgttt tctctcggtg ccagtatggt
    15121 gctcggtggt gtggcgcaga tgctggcacc gaaagccaga actccccgta cacagacaac
    15181 ggataacggt aagcagaaca cctatttctc ctcactggat aacatggttg cccagggcaa
    15241 tgttctgcct gttctgtacg gggaaatgcg cgtggggtct cgcgtggttt ctcaggagat
    15301 cagcacggca gacgaagggg atggtggtca ggttgtggtg attggtcgct gatgaaaaac
    15361 gtttatgtga aaccgcctcc gggcggtttt gtcgtttatg gagcgtgagg aatgggtaaa
    15421 ggcagcagta aggggcatac tccgcgcgaa gcgaaggaca acctgaagtc cacgcagttg
    15481 ctgagtgtga tcgatgccat cagcgaaggg ccgattgaag gtccggtgga tggattaaaa
    15541 agcgtgctgc tgaacagtac gccagtgctg gacagtgagg ggaatactaa catctccggc
    15601 gtcacggtgg tgttccgggc cggtgagcag gagcagacac cgccggaggg atttgaatcc
    15661 tccggctccg agacggtgct gggtacggaa gtgaaatacg acacgccgat cacctggacc
    15721 atcacgtcgg caaacattga ccgactgcgt gttaccttcg gcgtgcaggc actggtggaa
    15781 accacctcaa agggggacag gaatccgtcg gaagtccgcc tgctggttca gatacagcgt
    15841 aatggtggct gggtgacgga aaaagacatc accattaagg gcaaaaccac ctcgcagtat
    15901 ctggcctcgg tggtggtggg taacctgccg ccgcgcccgt tcagtatacg gatgcgcagg
    15961 atgacgccgg acagcaccac agaccagctg cagaacaaaa cgctctggtc gtcatacacc
    16021 gaaatcatcg atgtgaaaca gtgctacccg aacacggcac tggtcggtgt gcaggtggac
    16081 tcggagcagt tcggcagcca gcaggtgagc cgtaattatc atctgcgcgg gcgtattctg
    16141 caggtgccgt cgaactataa cccgcagacg cggcaataca gcggtatctg ggacggaacg
    16201 tttaaaccgg catacagcaa caacatggcc tggtgtctgt gggatatgct gacccatccg
    16261 cgctacggca tggggaaacg tcttggtgcg gcggatgtgg ataaatgggc gctgtatgtc
    16321 atcggccaga attgcgacca gtcggtgccg gacggctttg gcggcacgga gccgcgcatc
    16381 acctgtaatg cctggctgac cacacagcgc aaggcgtggg atgtgctcag tgatttctgc
    16441 tcggcgatgc gctgtatgcc gatatggaac gggcagacgc tgacgttcgt gcaggaccga
    16501 ccatcagata aggtgtggac ctataaccgc agtaatgtgg tgatgccgga tgatggcgcg
    16561 ccgttccgct acagcttcag cgccctgaag gaccgccata atgccgttga ggtgaactgg
    16621 attgacccgg ataacggctg ggagacggcg acagagcttg tggaggacac gcaggccatt
    16681 gcccgttacg gtcgtaacgt cacgaagatg gatgcctttg gctgtaccag tcgggggcag
    16741 gcgcaccgcg ccgggctgtg gctgattaaa acggaactgc tggaaacgca gaccgtggac
    16801 ttcagcgtgg gtgcggaagg gcttcgccat gtaccggggg atgtcattga aatctgcgat
    16861 gatgactatg cgggtatcag caccggcggg cgcgtgctgg cggtgaacag ccagacccgg
    16921 acgctgacgc tcgaccgtga aatcacgctg ccatcctccg gtaccacgct gataagcctg
    16981 gttgacggaa gtggcaatcc ggtcagcgtg gaggtccagt ccgtcaccga cggcgtgaag
    17041 gtaaaagtga gccgtgttcc tgacggcgtt gccggataca gcgtgtgggg gctgaagctg
    17101 ccgacgctgc gccagcgact gttccgctgc gtgagtatcc gtgagaacga cgacggcacg
    17161 tatgccatca ccgccgtgca gcatgtgccg gaaaaagagg ccatcgtgga taacggggcg
    17221 cactttgacg gcgaccagag cggcacggtg aatggtgtca cgccgccagc ggtgcagcac
    17281 ctgactgccg aagtcaccgc agacagcggg gaatatcagg tgctggcgcg ctgggacaca
    17341 ccgaaggtgg tgaagggcgt gagcttcctg ctccgtctga ccgtaacagc ggacgacggc
    17401 agtgagcggc tggtcagcac ggcccggacg acggaaacca cataccgctt cacacaactg
    17461 gcgttgggga actacaggct gacagtccgg gcggtaaatg cgtggggaca gcagggcgat
    17521 ccggcatcgg tatcgttccg gattgccgca ccggcagcgc cgtctcggat tgagctgaca
    17581 ccgggctatt ttcagataac cgccacgccg catcttgcgg tttatgaccc gacggtacag
    17641 tttgagttct ggttctcgga aaaacggatt gcggatatca ggcaggttga aaccagcgcg
    17701 cgttatcttg gtacggcact gtactggata gccgccagta tcaatatcaa accgggccat
    17761 gattattatt tttacgttcg cagtgtgaac accgttggca aatcggcatt cgtggaggct
    17821 gtcggtcagc cgagtgatga tgcatcaggc tatctggatt ttttcaaagg cgagataggg
    17881 aaaacccatc tggctcagga gctgtggacg cagattgata acggtcagct tgcgcctgac
    17941 ctgactgaaa tcaggacgtc cataacggat gtcagcaatg aaataacaca gaccgtcaat
    18001 aagaaactgg aagaccagag tgcagcgatc cagcagatac agaaggttca ggttgataca
    18061 aataataacc tgaacagcat gtgggcagtg aagctgcagc agatgcagga cggacgcctt
    18121 tatattgcgg gtatcggtgc cggtattgag aacacccctg acggcatgca gagtcaggtg
    18181 ctgctggcag cagacaggat tgcgatgatt aatcctgcga atggcaacac aaagccgatg
    18241 tttgttggtc agggcgatca gatattcatg aatgaagtgt tcctgaaacg cctgacggcc
    18301 cccaccatta ccagcggcgg taatcctccg gcattttccc tgacatcaga cgggagactg
    18361 acggcgaaaa atgcggatat cagtggcagt gtgaatgcga actcaggaac gctcaacaat
    18421 gtcacgatta accagaactg tacgattaag ggcatgctgg aggcgaccca ggtcagagga
    18481 gatttcgtta aagctgtatc aaaagccttc ccgaaaaaag tcggtacgtg gggtaacacg
    18541 gaaacaccaa acggtacggt tacagtcacc atcagcgatg atcataactt tgaccgccag
    18601 attattattc cgcccattat ttttaacggt atagcgtatg acgatccggg gagcggaaat
    18661 aacccaggag gcacgcgata cacgggttat ggttttgaag ttcgcaaaaa cggcgtatta
    18721 atcgcatcca gagaaactaa aggggccatt cccggtagtt acagtgcagt tattgatatg
    18781 ccgagtggca ggggaagcgt cactctggag tttaagattt tccagaaagg caatcagggg
    18841 gcaggcaata tcaccgactg tacggtgatt gtgaccaaaa aagccgcttc cggcatcagt
    18901 attcgttgaa atatttataa ccccaataaa gggcgtcagg aatgacgcct tttttattgc
    18961 agaaaagcga gaggtaatta tgcgtaaagt ttgtgcagca attttgtccg cagccatttg
    19021 tctggccgta tccggtgcgc ctgcatgggc gtctgaacat cagtccacgc tgagcgcggg
    19081 gtatcttcat gcctcgacga acgtccccgg cagcgatgat ctgaacggga ttaacgtgaa
    19141 ataccgttat gagtttacgg acacactggg gctggtgacg tcattcagct atgcaggaga
    19201 caagaatcgc cagctgaccc gttacagcga tacccgctgg catgaagatt ccgttcgtaa
    19261 ccgctggttc agcgtaatgg cggggccgtc tgtgcgcgtg aatgaatggt tcagcgcgta
    19321 tgcgatggcg ggtgtagctt acagccgtgt gtcgactttc tccggggatt atcttcgcgt
    19381 aactgacaac aaggggaaaa agcacgatgt gctgaccgga agtgatgacg gtcgccacag
    19441 caacacgtct ctggcgtggg gggctggcgt gcagtttaac ccgaccgaat ccgtggccat
    19501 tgatattgct tatgaaggct ccggcagtgg tgactggcgc actgacggtt tcatcgtggg
    19561 tgtcggttat aaattctgat tagccaggta acacagtgtt atgacagccc gccggttcag
    19621 gcgggctttt ttgtggggtg aatatggcag taaagatttc aggtgtactg aaagacggca
    19681 caggaaaacc ggtacagaac tgcacaatcc agctgaaagc aaaacgtaac agcaccacgg
    19741 tggtggtgaa cacgctggcc tcagaaaatc cggatgaagc cgggcgttac agcatggacg
    19801 ttgagtacgg gcagtacagc gttattctgt tggtggaggg attcccgccg tcacatgccg
    19861 ggaccatcac cgtgtatgaa gattcccgac ccggtacgct gaatgatttt ctcggtgcca
    19921 tgacggagga tgatgcccgt cctgaggcac tgcgccgttt tgaactgatg gtggaagagg
    19981 tggcgcgtaa cgcgtccgcg gtggcacaga acacggcagc cgcgaagaag tcagccagcg
    20041 atgccagcac atcagcccgt gaggcggcaa cccatgcgac tgatgctgca gattcagcac
    20101 gtgcagccag cacgtcagcc ggacaggccg cttcgtcggc tcagtcagcg tcttccagcg
    20161 caggaacggc atcaacaaag gccactgaag cctcaaaaag tgctgccgct gcagagtcct
    20221 caaaaagcgt ggcagctacc agtgccggtg cggcgaaaac gtcagaaacg aatgccgcag
    20281 cgtcagaaca atcagccgcc acttctgcat ccaccgcgac cacgaaagcg tcagaagctg
    20341 ccacctcagc ccgggatgcg gcggcctcaa aagaggcagc gaaatcatca gaaacgaacg
    20401 catccttgag cgccagtagc gcagcttcct cggcaacggc ggcaggaaat tccgcgaagg
    20461 cggcaaaaac gtccgagacg aacgccaggt cttctgagac ggcagcggga cagagcgcct
    20521 cagctgcggc aggctcaaaa acagcggctg cgtcgtctgc cagtgccgcg tcaacaagtg
    20581 ccgggcaggc ctcagccagt gccaccgccg ccggaaaatc ggcagaaagc gccgcatcat
    20641 ccgcttcaac agccacaacg aaggctggcg aagccactga gcaggccacg gcagcagcga
    20701 ggtcagcttc cgcagcgaag acatccgaaa cgaacgcgaa agcgtcggaa acccgtgcag
    20761 aatcctcaaa aacggcagcg gcatcgtccg ccagttcggc ggcgtcatcg gcatcatcgg
    20821 cgtctgcttc aaaagatgag gcgaccagac aggcgtcagc agcgaagggc agcgccacga
    20881 cggcatccac gaaggcgaca gaggctgctg gtagtgcgaa ggcagcagct cagagcaaaa
    20941 gtacggcgga atctgcagca acgcgcgctg agacagcggc aaaacgggca gaggatattg
    21001 catccgccgt ggcgcttgag gatgcgagca cgacgaaaaa ggggatagta cagctcagta
    21061 gtgcgactaa cagcacttcc gagtcactgg cggcaacacc aaaagccgtt aaggccgcgt
    21121 atgacctggc tagcgggaaa tacaccgcac aggatgcaac gacagcacag aaagggataa
    21181 tccagctaag cagcgcgacc aacagcacgt ctgaaacgct ggcggcaacg ccaaaggcag
    21241 taaaagcagc caatgacaat gctgagaaac gtctgcagaa agatcagaac ggtgcggata
    21301 tccctggcaa agacaccttt acgaaaaata ttggtgcctg ccgtgccttc ggtgggtcag
    21361 taagcacaac aacaggaaac tggacgactg cacagtttat cgagtggctg gattctcagg
    21421 gagcatttaa ccatccatac tggatgtgca agggttcctg gtcttatggc aataataaaa
    21481 tcattactga tactggctgc ggtaatattc atctcgccgg agctgtcatt gaagtaatgg
    21541 ggataaagtc agcgatgacg atccgcatta ccactccgac cacatccagc ggtggcggaa
    21601 caaccaacgc ccagtttacc tatattaatc acggaacaga ttattcacct ggctggcgaa
    21661 gggactataa ctccagaaat aagccaacgg catcagagat cggggcgcta ccgtcaggtg
    21721 gaacagcagt atcatcagtt aatctggctt caaaaggtcg ggtagccgcg ctgacagaca
    21781 atacgcaggg ggcagcaggt cttgagttat acgaggtgta taacaacgga tatccaacaa
    21841 cgtatggaaa tatcattcac ctgaaaggga tgacagccgt tggcgaaggt gagttactca
    21901 tcggctggag tggtacaagc ggtgctcatg ctccggcatt tattcgttca cgacgggata
    21961 cgaccgacgc aaactggtcg ccgtgggcgc agctttacac ctcggctcat cctcctgcag
    22021 agttttatcc agtcggtgca ccaatcccgt ggccatcaga taccgttccg tctggttatg
    22081 tcctgatgca ggggcagact tttgacaaat ctgcataccc gaaacttgca gccgcttatc
    22141 cgtcaggcgt gatccctgat atgcgtggct ggacgattaa gggcaaacct gccagtggtc
    22201 gggccgtatt gtctcaggaa caggacggca ttaaatcgca cacccacagc gccagcgcat
    22261 ccagtacgga tttggggacg aaaaccacat cgtcgtttga ttacggcact aaatccacga
    22321 ataacatcgg ggcgcatacg cacagtgtga gcggtacagc cgcaagtgcc ggaaaccata
    22381 ctcatagtgt cacaggcgca tcagcagtca gccagtggtc acaaaatggg tcagtacata
    22441 aggtagtgtc tgcggccagt gtgaatacaa gtgctgcagg agcgcacact catagtgtca
    22501 gcggcacagc tgcatctgca ggtgctcacg cacatactgt cggtattggt gctcatacgc
    22561 actcggttgc gattggctca catggacaca ccatcaccgt taacgctgct ggtaacgcgg
    22621 aaaacaccgt caaaaacatc gcatttaact atattgtgag gcttgcatga ttacgctcat
    22681 tctttctgca ccagtaaccg aaatggctga agcatttaag cgggtatttg caaatgcaga
    22741 taatgtgaat attgtcggaa agccatttga aacaatcaga gaatttgact gcatggtaag
    22801 tgcggcaaat agtttcggcc tgatggatgg cggtgttgat gccgccatta ccacattctt
    22861 cggttctcag ctacaggccc gcgttcagaa tcatattctt cgtgaatatc tcggcgagca
    22921 gcctgtaggt tctgcatttg ttattgaaac ggggcataat tatcacccct ggctggtaca
    22981 tgcgccaaca atgcgtgttc cgttgacaat tgacggaaca gacgctgtat ataacgcaac
    23041 ttgggcagct ctacttgcta tctttcagca caataaaaac gcaacgacag acaggaaaat
    23101 aaagacggtg gtattccctg caatgggagc cggatgtggt caggtgccgt ttgaaagcgt
    23161 tgcccggcag atgaagcagg catgggataa ctttaataaa aaaacagaat caattaactg
    23221 ggaatacgca caatcccgcc agtcggcagt atttggcaca tatgcatact gtccgggtaa
    23281 ttctgtttgc cgttatgcgg atactaaata tattggatgc ggcgattatc ggacgtattg
    23341 ctcacgttcc gggcaggtct gtattaaccc tgaacatcag gctgatgatg tactgataca
    23401 acatcaggct aataaccggt ttcgccctgg ttcgcatata caccggatta atccagaaaa
    23461 tcctgtaggt aatgtcatct ctggcgcaca tagccacgga agtagcatcg ttattggtgc
    23521 tcacacccat acgctcaata aacaatattc tgtctctgat attaagtaga ggtgaacatg
    23581 gatttcagaa tgagtgaaca accacggacc ataacaattt ataatctgct ggccggaact
    23641 aatgaattta ttggtgaagg tgacgcatac attccgcctc atacaggtct gccagcaaac
    23701 agtaccgata ttgcaccgcc agatattccg gctggtttcg tggctgtttt caacagtgat
    23761 gagtcatcgt ggcatctcgt tgaagatcat cggggtaaaa cggtttatga cgtggcttcc
    23821 ggcgacgcgt tatttatttc tgaactcggc tcattaccgg aaaatgtcac ctggttatcc
    23881 ccggaagggg agtttcagaa gtggaacggc acagcctggg tgaaggatac ggaagcagaa
    23941 aaactgttcc ggatccggga ggcggaagaa acaaaaaaca gcctgatgca ggtagccagt
    24001 gagcatatag cgccgcttca ggatgctgtg gatctggaga tcgcaacgga gaaagaaacc
    24061 ttgttgctgg aagcctggaa aaagtatcgg gtgttgctga accgtgttga tacatcaact
    24121 gcaccggata ttgaatggcc agccctaccg tagggtaaga tatataaatt ctataattag
    24181 aagtatcttt ccatttaagg ctaggaaggg gggcttggaa aacgtaagga atctcacacc
    24241 gagattattt ttatatatca ggtgtctgat tttttgcttt agctcttaaa atggtttgcc
    24301 gcgaggtttt gaattcttgg gcaatgtcac ttatatttat acctgactta atttgctcta
    24361 gtaccctctg tatttgttca tcatttaaca caggtgggcg accaaatcgc tttcctgcgc
    24421 cccgtgctct taatatccct gaatgagtac gctcaagtaa aaggtctcgc tcaaattcag
    24481 cgactgctga aattacttgc atcatcattt ttccaggtgg actggtcagg tcaacgcccc
    24541 ccaatgctaa gcaatgcact ttgatacctg ctttggtcag ttgttccacc gttttactga
    24601 tatccattgg attacaaccg aggcgatcca gttttgtgac aatcaatgca tcacctcttt
    24661 tcagtcgatc aagcaactgg ttaaaaccag gacgctcact ggttgctgct gagccgctaa
    24721 tttgttcttc aattatttgc tgagatttga tgttaaaacc tgcactttcg atttcccgac
    24781 gttgattttt ggttgtcttt tccagagttg atacccgaca gtaagcataa attcgagaca
    24841 tagtgagatc ttctata
//
LOCUS       DAZGAP010000072        24465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGAP010000072 DAZGAP010000000
VERSION     DAZGAP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.072"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..1509
                     /gene="yicJ"
                     /locus_tag="ACHLAM_002415"
     CDS             127..1509
                     /gene="yicJ"
                     /locus_tag="ACHLAM_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO7853931.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1519..3837
                     /gene="yicI"
                     /locus_tag="ACHLAM_002416"
     CDS             1519..3837
                     /gene="yicI"
                     /locus_tag="ACHLAM_002416"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO7853932.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEMPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLMVTPQGNALTITL"
     gene            4133..5686
                     /locus_tag="ACHLAM_002417"
     CDS             4133..5686
                     /locus_tag="ACHLAM_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO7853933.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENITDLLHLDHLLVAECEEIDGVMNNSRRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            5713..6183
                     /locus_tag="ACHLAM_002418"
     CDS             5713..6183
                     /locus_tag="ACHLAM_002418"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO7853934.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            6201..6515
                     /locus_tag="ACHLAM_002419"
     CDS             6201..6515
                     /locus_tag="ACHLAM_002419"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO7853935.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            6540..7631
                     /locus_tag="ACHLAM_002420"
     CDS             6540..7631
                     /locus_tag="ACHLAM_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO7853936.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            7643..8494
                     /locus_tag="ACHLAM_002421"
     CDS             7643..8494
                     /locus_tag="ACHLAM_002421"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO7853937.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            8575..9435
                     /locus_tag="ACHLAM_002422"
     CDS             8575..9435
                     /locus_tag="ACHLAM_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO7853938.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            9483..10415
                     /locus_tag="ACHLAM_002423"
     CDS             9483..10415
                     /locus_tag="ACHLAM_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7853939.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            10396..11076
                     /locus_tag="ACHLAM_002424"
     CDS             10396..11076
                     /locus_tag="ACHLAM_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO7853940.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(11120..12829)
                     /gene="yicH"
                     /locus_tag="ACHLAM_002425"
     CDS             complement(11120..12829)
                     /gene="yicH"
                     /locus_tag="ACHLAM_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7853941.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGSWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVTGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(12950..14341)
                     /gene="xanP"
                     /locus_tag="ACHLAM_002426"
     CDS             complement(12950..14341)
                     /gene="xanP"
                     /locus_tag="ACHLAM_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO7853942.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            14620..15825
                     /gene="gltS"
                     /locus_tag="ACHLAM_002427"
     CDS             14620..15825
                     /gene="gltS"
                     /locus_tag="ACHLAM_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO7853943.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(15859..17940)
                     /gene="recG"
                     /locus_tag="ACHLAM_002428"
     CDS             complement(15859..17940)
                     /gene="recG"
                     /locus_tag="ACHLAM_002428"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO7853944.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(17946..18635)
                     /gene="trmH"
                     /locus_tag="ACHLAM_002429"
     CDS             complement(17946..18635)
                     /gene="trmH"
                     /locus_tag="ACHLAM_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO7853945.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(18642..20750)
                     /gene="spoT"
                     /locus_tag="ACHLAM_002430"
     CDS             complement(18642..20750)
                     /gene="spoT"
                     /locus_tag="ACHLAM_002430"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO7853946.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(20769..21044)
                     /gene="rpoZ"
                     /locus_tag="ACHLAM_002431"
     CDS             complement(20769..21044)
                     /gene="rpoZ"
                     /locus_tag="ACHLAM_002431"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO7853947.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(21099..21722)
                     /gene="gmk"
                     /locus_tag="ACHLAM_002432"
     CDS             complement(21099..21722)
                     /gene="gmk"
                     /locus_tag="ACHLAM_002432"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO7853948.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            21980..23665
                     /gene="ligB"
                     /locus_tag="ACHLAM_002433"
     CDS             21980..23665
                     /gene="ligB"
                     /locus_tag="ACHLAM_002433"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO7853949.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARAKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(23662..24279)
                     /gene="yicG"
                     /locus_tag="ACHLAM_002434"
     CDS             complement(23662..24279)
                     /gene="yicG"
                     /locus_tag="ACHLAM_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO7853950.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
BASE COUNT         5759 a         5969 c         6619 g         6118 t
ORIGIN      
        1 ctaccacgat cacataaaac aagaatcata aattaataac cagatatcgg aatattcgct
       61 ctcgcaggga tggtttacaa aatgcgttat cggtctcagc acccctatag catcaaggaa
      121 aagcagatga agagtgaagt gttgtccgtt aaagagaaaa ttggttatgg catgggagac
      181 gccgccagcc acattatttt cgataacgta atgttatata tgatgttctt ttataccgat
      241 atttttggca ttcctgccgg atttgtcgga accatgtttt tggtcgctcg tgcactggat
      301 gcgatttccg atccttgcat ggggttgttg gccgatcgaa cgcgctctcg ctggggtaaa
      361 tttcgtccgt gggtactgtt tggcgcactg ccatttggga tcgtctgtgt actggcctat
      421 agcacgccag atctcagtat gaacggaaaa atgatttatg cagcaattac ttacacccta
      481 cttaccttac tttataccat cgttaatatc ccttactgcg cattgggtgg tgtaatcacc
      541 aatgacccga ctcagcgtat ctcgctgcaa tcctggcgtt ttgtgctggc gaccgcggga
      601 ggcatgctct ctactgtgct gatgatgccg ctggttaatt taattggtgg tgataataaa
      661 ccactcggtt tccagggcgg tatcgcggtc ctttccgtgg tggcattcat gatgctggca
      721 ttctgtttct tcaccactaa agaacgcgtt gaagcaccac ctacaacgac gtctatgcgg
      781 gaagatttac gtgatatctg gcaaaacgac cagtggcgga ttgtcggttt actaaccatt
      841 ttcaatattc tggcggtgtg cgtacgcggt ggagcgatga tgtattacgt cacatggatt
      901 ttgggtacgc cggaagtgtt tgtcgctttt ctcaccactt attgcgtggg taacctgatt
      961 ggttccgcac tggcaaaacc gctgaccgac tggaaatgta aagtcactat cttctggtgg
     1021 acgaacgccc tgctggcagt gattagcctc gcgatgttct ttgttcccat gcaggccagc
     1081 atcactatgt ttgtcttcat cttcgtgatt ggtgtattgc atcaactggt gacacctatc
     1141 cagtgggtaa tgatgtccga taccgtcgac tacggcgagt ggtgcaatgg taaacgcctg
     1201 accgggatca gttttgctgg cacgctgttt gtgctcaagc tggggttggc cttcggcggc
     1261 gctcttatcg gctggatgct ggcttatggc ggatatgatg cggcagaaaa agcgcagaac
     1321 agcgccacga ttagcatcat tatcgcgcta ttcacgattg ttccggcgat ctgttacttg
     1381 ctgagcgcga ttatcgctaa acgctactac tcactcacga cgcacaatct gaaaaccgtt
     1441 atggaacagc tggcgcaggg caaacgccgt tgccagcaac aattcacctc tcaagaagtg
     1501 cagaactaag gaacggcaat gaaaattagc gacggaaact ggttgattca acctggcctc
     1561 aatttgattc acccgcttca ggtgttcgag gttgaacagc agggtaatga aatggtggtc
     1621 tatgctgccc cccgtgatgt gcgtgaacgt acctggcagc ttgatacacc tttatttacg
     1681 ctgcgctttt tctccccaca ggaaggtatt gttggtgtac ggattgagca ttttcagggg
     1741 gcgctgaata acggtcctca ttatccgctc aatattttgc aggatgtgaa ggtcacaatc
     1801 gaaaacacag aacgttatgc tgagtttaaa agtggcaact taagcgcgcg tgtcagcaaa
     1861 ggtgagttct ggtcactgga ttttctgcgc aacggcgaac gtattaccgg tagtcaggtg
     1921 aaaaataatg gctacgtgca ggacacgaat aatcaacgaa attatatgtt tgagcggctg
     1981 gatcttggcg ttggcgaaac agtttacggt ctgggagagc gctttactgc cctggtgcgc
     2041 aatggccaga cggtagagac ctggaaccgg gacggcggca caagtactga acaggcgtat
     2101 aaaaatatcc cgttctatat gactaaccgt ggttatgggg tactggtcaa tcatcctcaa
     2161 tgcgtctctt ttgaagtggg atcggagaaa gtctccaaag tgcagttcag cgttgagagt
     2221 gaatatctcg aatactttgt tatcgacggc ccgacgccga aagcggtact tgatcgttat
     2281 acccgtttta ctggtcgtcc ggcgctgccg cccgcgtggt ccttcggcct gtggctaacc
     2341 acttcattta ccaccaacta cgacgaagcg acggtaaaca gctttatcga tggtatggcg
     2401 gaacgcaatc tgccgctgca tgttttccac tttgactgtt tctggatgaa agccttccag
     2461 tggtgcgatt ttgagtggga cccgctgact ttcccggacc cggaagggat gatccgccgc
     2521 ctgaaagcga aagggctaaa aatctgcgtc tggattaacc cctatattgg gcaaaaatcc
     2581 cctgtcttta aagagttaca agagaaaggc tatttactca aacacccgga cggttcgtta
     2641 tggcagtggg ataaatggca gccaggtctg gcaatttatg actttaccaa tccggatgcc
     2701 tgcaaatggt acgccgacaa actgaaaggt ctggtcgcga tgggcgttga ttgctttaag
     2761 accgactttg gcgaacgtat tccaaccgat gttcaatggt ttgacggttc cgatccgcag
     2821 aaaatgcata accattatgc gtacatctac aacgaactgg tgtggaacgt gctcaaggac
     2881 accgttggtg aggaagaagc tgtcttgttt gcccgctcgg cttccgtcgg tgcgcagaaa
     2941 ttcccggtac actggggtgg cgactgttac gctaactacg aatcaatggc ggaaagcctg
     3001 cgcggtggtt tgtctattgg cctttcaggt tttggcttct ggagccacga tatcggcggc
     3061 tttgagaata ccgcgccggc gcatgtttac aaacgctggt gcgcgtttgg tttgctctcc
     3121 agccatagcc gtttacatgg cagcaaatct tatcgtgtgc cgtgggccta cgatgatgag
     3181 tcctgtgatg tggtgcgctt cttcacgcaa ctgaaatgcc gcatgatgcc gtatctgtat
     3241 cgtgaagctg ctcgtgcgaa cgcgcggggt acgccgatga tgcgggccat gatgatggag
     3301 tttccggacg atccggcttg tgattacctt gaccgtcaat acatgttagg cgacaacgtg
     3361 atggttgcgc ctgtgttcac tgaagcgggc gatgtgcagt tctacttgcc tgaaggtcgc
     3421 tggacacacc tgtggcacaa cgatgaactc gatggtagtc gctggcataa acagcagcac
     3481 agcttcctga gtctgcccgt ttatgtgcgt gataacaccc tactggcgct gggcaacaac
     3541 gagcaacgtc ccgattacgc gtggcacgaa ggcacggcat tccagctctt taatctacaa
     3601 gacgggcatg aagccgtctg tgaaatgccc gctgctgacg gttcggtgat ctttactcta
     3661 aaagcaacac gtactggtaa cacgattact gtgactggtg cgggcgaggc gaagaactgg
     3721 acactgtgct tgcgcaatat tgtgaaagta aatggtctgc aaggcggttc gcaggctgaa
     3781 agtgagcagg ggctgatggt gacgcctcaa gggaatgcgc tgacaattac gttgtaatct
     3841 attgatggaa tgccgggtac gaatggtttt gtgtcgtatc cggtaaataa tctcacatct
     3901 ggtaataaat atttcttcca tcgtatttgc gagcatgatc acgattattc atcgcgaaaa
     3961 tgtgatctgt gctgaaaaat atcccttaac cgccttgctt tgttttatca ctgtccggat
     4021 tacggacaga ttttagtatt gttgtgaaat tattctgctt tatatttacc ctcagagatg
     4081 gcaactgaag ctcttcctca tgaggcttct taatctgtga taagggtaaa aaatgcaaat
     4141 gattacctcg cgacaaaaca gattattacg attcctttta cctcgaaggg aatatacgac
     4201 tattgttacg attgccggct atttaaatgt ttcggaaaaa accattcaac gtgatttacg
     4261 tttacttgag caatggctgg gacaatggag aataaatgtt gagaagcgtg ctggcgcggg
     4321 tgtgatgtta agcgcggaga atattactga tttgctgcat cttgatcatt tgttggtggc
     4381 agaatgtgaa gagattgatg gtgtaatgaa taattccagg cgcgttaaaa tagcgtcgca
     4441 gttattaagt gaaacaccga atgaaacgtc gatcagtaaa ttgtcagaac gctactttat
     4501 cagcggagcc tctattgtta atgacctgag agtaattgag tcctggcttg cgccgttggg
     4561 gttatcgttg atccgcagtc caagtggtac gcatattgaa ggtagtgaag ggcaagtccg
     4621 acaggcaatg gcattactga ttaacggcat tattaaccat aatgagccgc aaggtgtcgt
     4681 gtattcacgt ctggatcccg gaagctataa agcattagtc cattattttg gagaagaaga
     4741 ggtgttattt gtccagtcat tgttactgga tatggaaaat gaattaagtt ggtctttggg
     4801 agaaccttat tacgttaaca tttttactca catccttatt atgatgtatc gcaacacgca
     4861 cgggaatgcg ttatcaagag aagaagatca aaccaggcaa tatgatgaaa atatctttaa
     4921 tgttgccagt cagatgattc ataagataga acaacgaatt gcacatacat tgcccgatga
     4981 tgaagtctgg tttatttatc aatatatcat ttcatcaggt gtggcgattg atggacaaaa
     5041 agatgttagc attatttcac atatgcaggc cagcaatgaa gcgcgtctga ttacctggcg
     5101 tttaattacg gtattcagtg acatcgtgga ctgcgatttt agtgaagaca gcgcattata
     5161 tgatggctta ttggtgcata ttaaaccgct gattaaccga ctaaattatc gtattcatat
     5221 ccgtaatcca ttgttggaag atattaaagc agaactagcg gatgtctggc ggttgacgca
     5281 atatgtggtg aatcaggtat ttaaaacctg gggtgagaat gcagtgagcg aggatgaagt
     5341 aggttacctg accgttcatt ttcaggctgc gatggagcgg caaattgccc gtaaacgcgt
     5401 attactggtc tgttcaaccg gaatcggaac ttcgcatcta ctgaaaagcc gtattctgcg
     5461 agcatttcct gaatggacga ttgttgatgt tatttcagca gcgaatttat cacaggtttt
     5521 gcctgacaat atcgaactga ttatttcgac cattaatttg cctacagtca ctatgccggt
     5581 cgcttatgtt accgcttttt ttaatgatgc cgatattaag cgggtcactg aaatggtgat
     5641 tacggaaaaa ttacatcatg cgacatctcg ggtcgttgaa atttaatgtc agaattttat
     5701 gaggtatgac gaatggacat aacaaaaatc ctgaatacga atcgcgttat tttagatatg
     5761 caagcgacga ataaagagga ggcgattgaa gaattaacta atcttctcaa aaaagatggt
     5821 gcgatcgatt gtcgggaaac ctttattaaa gatgtctggc agcgcgaagc ggaaggctcg
     5881 actggttttg aaaatcatat tgcgatcccg catggtaaat cttcagctgt gattaatact
     5941 acgctggcaa ttggccgtac ccggcaggat attccctggg aaacgctgga tggcagtagc
     6001 gtgcgctgta ttattttatt tgcggtacgt cttgaagatc aaaataccac ccatatccgt
     6061 ttactttccc aggtcgcggg cgcgctggcg gatgatgaca tcatagaaca attgttagtt
     6121 gagacgagtc cgcaaaaaat cattgatctc tttagtcagt atgcacaatc cgatgtctgc
     6181 taaataacag ggagtatgtc atgaatattg tctgtgttgc cgcctgtacg gcgggtattg
     6241 ctcataccta tattgctcgt gaaaagttaa tcaaaggagc aaaagctctg ggtcatacca
     6301 ttaaagttga aacccaggga accattggta cagaaaatga attacaggcg gcagatatct
     6361 ctgctgctga cgtcgtgatt cttgctgtag atgtgaaaat aaaaggagaa gaacgcttca
     6421 ccaataaacg tattgtgcga gtaaagacag agatcgtcat taaatcgccc gttcaatttc
     6481 ttgaaaaggt cgaaaaatca ctgggaaaca aatgatcctt atactggagg gatcttaata
     6541 tgaaagaaaa taaaattccg gtttcacagg aaattaaaaa acatttatta accggcatct
     6601 cgtggatgat accccttatt gtggccgccg ggatatgtat tgcgttgggg caagttcttg
     6661 gtggtacaga tgttggtgaa aaaacaggaa caatcccgtg gatgcttaat cagattggcg
     6721 gctggggaat ggggctgatt gtgccgctga ttagcgcggc aattgcctat tctattgccg
     6781 atcgtcccgg atttgccccg ggcctgattg tcggctttct ctgtgggcaa atccataccg
     6841 gatttattgg cggtatgttg ggtggtttcc tggtgggtta caccattttg ctacttaaac
     6901 gctatatccg cctgccgcag tcgatgcaag gactcatgcc aatcatggtg ttgccggtat
     6961 taagcaccat tatcggtggg ctgttaatga tgacgctgat tggtaaaccc atcgcctggt
     7021 tacaagaagc actgattcat ttgctcgagt cgatgcaggg cggatcgcgc ttcctgatgg
     7081 gggccatact tggcgcgatg gcaaccttcg atttcggtgg tccggtcaac aaaaccatgt
     7141 cgctgttttc cgatggcttg ctggtcagtg gcgtctacgg accagaagcc gtgaaatttg
     7201 tcggctcaat catccctccg tttggcatca cactttcgtt cttactgaca cgccataaat
     7261 acacccgtgc ggagcgcgag gcgctgaaag ccgcgtttcc gatggggatc tgcatgatca
     7321 ctgaaggagt cattccgatc gccgcgcgtg acctgctgcg agtagtggga tcttgtgtgg
     7381 tggcatcggc ggtcgcgggc ggcttaatca tggtgtgggg cgtggagagt ccggttccgc
     7441 atggcggcat gtttgttgtg cctctgttca cccatcctct gctgttttgc ctggcgctgg
     7501 gtataggcac ggtgatttgt ggcgtgatgc tttcactctg gaagaaaccg gtaacggaac
     7561 gcgacgaaga gtttgacgaa ctgagcgatc aaaagctgaa agatgaagag atcactttca
     7621 ccctcgaata accggaggcc ctatgtttgc cgatatgaaa agtatggtga tgaaagcctg
     7681 gcatgaacat tacgctttgc tggcgatcaa ctgtatgaat ctggaaagtg ctcacgcagc
     7741 tatccgcgtg gcggagaaga atcgcgctcc gatcattctc aatctctatc aggggcatct
     7801 ggcgcatttt cctgcacccg tcgcggcggc agtggttaaa acgctcgcgg aatctgcctc
     7861 agtaccggtc gctctggcgc ttgatcatgg caaaaatccg gactgtattc gtcaggcgtt
     7921 tcgtgctggt ttcagcggat taatgattga tgcttctgct tttcccctgg aggaaaacgt
     7981 gcgccagacc agggcggtgg tggaactcgc cgccagcgtg cagctctgcg tggaaggcga
     8041 actgggccat ctggcagatg cgccgcgtta cgaccaggcc gccaatgcag acctgatgac
     8101 gcaacccact gatgtggaaa cgtttatcgc gcagacaggc attgatctac tggccgtctc
     8161 ggtaggcacg gcgcacggca tgtacgcgcc tggtgttgta cctgctattg attttcagcg
     8221 tctggcggag atttcgcgct gctcgagcgt gcctctggca ttacatggcg gtagcggtac
     8281 gccgttcgat cagctccagc tctgtacgac gctgggggta gcaaaaatca acgtgggggc
     8341 ggccattttt gaacggggga aatccgcgct cctgcatacc ctctgtcacg atatatccat
     8401 tgaactggtg gacgcgctga aagctatgga actggccttc gaagaagcga ttacaccgta
     8461 tttacaggcg agcggcgcta tcggcaaagc ctgattcatt ttctgtcatg gcctttatgc
     8521 cagtgcaata acgcaccggc agggcggact acacgctgaa actggagatt taacatgtta
     8581 gtttcgatga aagaattact gcaacctacc cgccagcacg gctttgccat tggcgcattt
     8641 aatgtggcgg ataactgttt ccttcgcgca gtcgtggatg aagcagaagc gacaaatact
     8701 ccgacgatta ttgccatcca tcccagcgaa catgattttg tgggcgatgc ctttttcgct
     8761 cacgttcgcg aaattaccca gcgtagtccg gtcccgtttg tactgcacct cgatcatggt
     8821 gcctctgtag agcatgtttt gcgtgcgatt cgctgcggat ttacctcagt aatgatcgac
     8881 ggttcgctgc tgccatatga agaaaacgtg gcgctgaccg cagaagtggt tcgcctcgct
     8941 cacgcggttg gagtatcggt tgagggtgaa ctgggaacca tcggacaaac aggaacgtcg
     9001 gtagaaggtg gtgtttcaca agtgacctac acagatccgg cgcaggccgc cgattttatt
     9061 gctcgtacag gtgccgacac actggctgtg gcgattggta cagcgcatgg catctatcca
     9121 aaagggatgc agccgaagct gcaaatggac attttgcgcg acattgccgg acgtattgat
     9181 attccgctgg tgctacacgg tggttcggca aacccggacg cggagattgc tgaatcagtt
     9241 acgctcggcg tcggcaaaat caatatctcc agcgatatga aatacgccta tttccagaaa
     9301 gtgcgtgaaa ttctcgctaa agagagctgg tgggacccga acgtcatcta tccggatgcg
     9361 attaacgcag cgcgggaagt cattcgccac aaaatgaaac tgttcggttc gctcggtaaa
     9421 gcatcactgt actaagccag aagggcgggg tgacccgcct tttacgcaac aaggaacgag
     9481 atatgcacta tctggggttg gatattggtg ggaccaaaat cgccgccgtc gtcatggatg
     9541 cgcatggctg ggagattcgc cgttaccgct gcccgacgca aaagtcgaca tatcaacaat
     9601 ttgtctcatg cgttgtggcg cttatcgagc agattaggcg ggacgttcaa cgaccgatgc
     9661 tgacagggat cgccttaccc ggcagtatct cgccactcac tggcctgatt aaaaacgcga
     9721 atattcaggt gattaacggt catgcgttac aggctgattt gcagcaattg cttgggcaac
     9781 cggtggtgat agccaatgat ggtaactgtt tcgcgctatc agaagcttgc gacggtgccg
     9841 ggcaagatta tgacgtggta tttggtatta cgcttggtac gggctgcggc ggtggcattg
     9901 ccatcaagca acgaccgttt ataggggcct ggggaaatgc tgccgaatgc ggtcatatca
     9961 cgttgccagg ctatacggag caggaagatg gtccatcagt cagttgctat tgcggcaaac
    10021 acaactgcgt ggagtcgttt gtttccggca gcggttttag tgaacgctat caacagatga
    10081 ctggtaactt gctcactcct gcggcgattg tcaccctggc acaacgtggt gatgcttgtg
    10141 ccatgcagca ggtggcacgt tttcgccaac agcttgcccg cacgctggca accatcgtta
    10201 atgttgttga ccctggcgtg attgtgatcg gcggcgggct ttcgaatgtg gaactgctta
    10261 tcaccgatct gaacgcagaa gtcgctccac tggttttcac cgatcaattc accactccca
    10321 ttgtaaaagc acagcacggc gacagtagcg gaatgcgtgg cgctgcctgg cttgctatgc
    10381 gcaacggaga agccaatgaa acgttcacaa attaattatg ttattgataa agcacatgcc
    10441 attgcgcaga ccttccgcgt ttgtttgcca gagttcgcct attttacggt ggatgactgg
    10501 ctgcagcggg aacgggataa ctggcaagaa gtggtcgact tacagctcgg ttgggatatc
    10561 accgattttg gtcgtggtga tttcaaccaa acgggactaa cactgctaac gatgcgcaac
    10621 ggcgcgctgg ggtcggcggc atatccaaaa ccgtatgctg agaaaatgtt gcagatccaa
    10681 caagatcagc agactccctg gcattttcac acccacaaaa tggaagatat cctcaatcgg
    10741 ggcggtggag acttatgcat gcagttggcc tgggccaacg aagccaatct ctgtgatgat
    10801 caacgagtca ttaccgtgag tgttgatggt cagcggcgta ccatgaaacc tggtgaaacg
    10861 ctggtgctaa agccggggca gggcatttgc ctgccgccgc gtctgtatca tcgcttctgg
    10921 gcggaaaaag cgtttgttct cggctgggaa atctcgatgg taaatgatga ccaacacgat
    10981 aattattttc ttgagccggg aggacgtttt ccggcgatag aagaagatga accggtgaaa
    11041 tggttattgt gcagtgagta tggaattttg cgctaataac ggggaacgct agcgctgata
    11101 tccgctagcg tccttatttt tactgcaccg gctccgtcga cgtaacttca ccactggaaa
    11161 ctacgcccgc attcatggtt tgagtcactt gctgctttgc ggcattcacg gcatgcaatt
    11221 gcccggtaac cgtaggtttc agcgggacat tggcctgaat atcgccactg gcagtaagct
    11281 gaatattgcc gtcgccagtc aacggtaatt caggccatcc ccactgttgc aaaatattca
    11341 ccggcacacc gcgtccattc aggctgatat gggtctgacg ttgtggcgtt tgtgaaacac
    11401 tggcggtggc ttccagaatg cctttttcag taaatgcact cagttcgctg atattcaccg
    11461 tgctgctgtt ggcggtcagc gccagcgatg ggcgacgaac atcaacacga ttgaatgtcg
    11521 cggcggcggc attcagattc gccgaaccac tccagacgcc ccatttatga tcggtaacca
    11581 gcgtcaggtt ggcaccgtaa ccatcgagcg cggtgagctg ccacgggaag tcagggtcga
    11641 tatcaatgat cagattgcgg ctggcgctaa atctcttcag ttgcaggcta tttaaccaac
    11701 cgggtgtcgt ttccatccac aactgttgcc agttttttgg caaggtatat tccagcccgg
    11761 caattgccgc atcatcaagg agtaacgttt tcccgtcacg caaccagctc cctgacgttc
    11821 tgaccatacc cccttcccag cggctggtga actggcgcag cgctacgccc tgcggggaaa
    11881 attccgcgtt tataatcggg tcaaataaat gcagcgaacc ataaatgaac tcgctagcgt
    11941 tcatcgacag tttgccttcc tgtgtctgcc agtcatcttt actgaaggtc atgttgcgca
    12001 agctgagatc gaggtcggtc accgcccagt ccggaccttg caaacgagcg tcaatgactt
    12061 ccaggcgacc aatctgcaac gacgggacag agcgtaatgg cgcaaagaag tcggttagcg
    12121 atttttcgct ttgtagacgg atatccgcca tgcgcagatt ttccacttgc cagctgccgt
    12181 cggcgttacg ctgcgcgttt ccggttaatg tcccgcgggc gatgtcggca cccaagttag
    12241 tcagcgtaac gcgatcgtta tcaatactgc cttcaatcag tacattggtg gcaggaacat
    12301 cattgagcga aagcgatccg gcactaaact gaatctgcgc cttcgtaccc agcactttac
    12361 cggcttctgg tgaccacgga accacgccac catttacccg ctgcgcgctt agtttccatt
    12421 cgctattcgg gctattaaac gccatatcac gcagttgcag acgatcggct ttgaacggta
    12481 gcggcgcggt ctggtcggtg agattcagcg tcccgttttc cagcaagatg gtatcgacat
    12541 ggcgtggttc ggtcagttgg cgactgctta gcgcaatgtc gacacttttg gccaccaggg
    12601 tcgcgggctg accatcacga ccaaacgtga cgttctccag cacgatatga gatggcgcgg
    12661 aaaaacggtg atccatcgcc ccgaaggcca gatgatagtc gctattctcg gaaacccatg
    12721 cgctgatatg ttctgctccc cagcgggttt gcagaagaaa atagaggcca gcgatcacca
    12781 ctaacagagc gatgagaatg tagagaagca gcttcccaat aaatttcatg gtcttccatc
    12841 ccgtgaaaag cacataaggg agttatgcac gatttacgcg caatcctcaa ggcgggaatg
    12901 gtgaaagacg tgagactgcg gcggtaatta tcttaccgcc gccagtgaat tactgttttt
    12961 ctggtgggaa aatcagattc agcacgatgg cagtaatacc gcccgcggca atcccggagg
    13021 agagcaggtt tttcagccat tcaggggcaa actgcaaaat cagcggctgc tgagagacgc
    13081 ccagaccgac cgccagcgac agcgcgataa tcagaatcgc ccgacggttc agcggctcac
    13141 gagaaacgat acgcacaccg gaggcggcga tggtgccaaa cattacaagc gttgcgccgc
    13201 ccagaaccgg ttcaggaatg tgttgtacaa aaccgctcac tgccgggaac agacccagca
    13261 cgatcaacat cagcgcgacg acaaaaccga catagcggct ggcaacaccg gttaactgga
    13321 tcactccgtt gttctgcccg aagcaggagt tcgggaaggt gttaaacacc gccgaaacaa
    13381 acgagttcag gccgtttgcc agcacaccgc ctttcaggcg tttcatgtac agcggaccgg
    13441 acactggctg ttcggaaacg tcagaggtcg ccgtgatatc gccaatggtt tccagcgaag
    13501 tgatcataaa gaccagcatc agcggcagca gcagactcca ttcaatgcca agaccgtaat
    13561 agagcggcgt tggcaccata atcagttctt gtgtcatcgg ttcgttgctt tctggcaaca
    13621 tgcccataaa ccacgccagc gcatatccgg ccgccatcgc aattaccagt gaggccacgc
    13681 gtaagtaagg gttacgttga cggttaagca ggataattaa ggctaaaacc acgcctgcca
    13741 gcagcagatt tttcggtgcg ccgaaggtgt tatcgctcat ggctgcgtaa ccgccgccaa
    13801 tggacgttaa cccaacctga attagcgaca ggccgataat catcaccaca acgccagaaa
    13861 ctagcggcgt aataatgcgg cgcgccagat gcagaacgcg ggagatcacc atctcagtgc
    13921 aacttgccag catcaacgtg ccgaacaaag ccgccatcat ggtaggaaca tcagcaccac
    13981 cggttttcag cgcggtaccg cccataatca gtggggcaac aaagttgaag ctggtgccct
    14041 gaatagacaa cagcccggag ccaaccggac cccaggcctt aatttgaata atcgatgcca
    14101 caccggaggc aaacagcgac atactaataa tgtgttgcgt gtcttgtgcc ggtaaaccca
    14161 gcgcctggca gattaatagc gctggcgtga tcaccgcaac gaacatcgcc agcagatgct
    14221 gacaggcggc aaacagagtt tgaggaagcg gcggacgatc ttcaagacgg taaatcagtt
    14281 cgctgttttg agtctgcgca accggttgcg cattttctga ctcgagggtg gaaacagaca
    14341 tcgaaaacaa tcccgttgta gcaaagcggg cattttagct gaccgaaaga gaaaagcaaa
    14401 cgtttgccac ggcctctgct aaatatttaa atgagataaa tgcacttttt tatataactt
    14461 ttggtatttt tctgcttaaa atccatgcca tttgttcatt aacagctgag aatttgaccc
    14521 aggaaagcat aactcgatac ccgtcatagt tcataagttg ccactgcgcg atgaagtatg
    14581 acgagtatga aagagtgatg cggatacaaa ggagtaacta tgtttcatct cgatacttta
    14641 gcaacgcttg ttgccgcaac gctgacgttg ctgctcgggc gtaagttggt ccattccgtc
    14701 tcctttttga agaaatacac cataccggaa cctgttgcgg gtggtttgtt ggtggcgctg
    14761 gcgctactgg tactgaaaaa aagcatgggc tgggaagtca actttgatat gtccctgcgc
    14821 gatccgttaa tgctggcatt cttcgccacc attggcctga acgccaacat tgccagtttg
    14881 cgtgccggtg ggcgtgtggt tggcatcttc ttgattgtgg ttgttggtct gttggtgatg
    14941 caaaatgcca ttggcattgg tatggctagc ctgttagggc ttgatccgct gatggggctg
    15001 ttggccggtt ctattacgct ttccggcggt cacggtacgg gcgctgcgtg gagtaaattg
    15061 ttcattgaac gctatggctt caccaatgcg acggaagtgg cgatggcctg tgcgacgttt
    15121 ggtctggtgc tgggcggctt gattggcggt ccggtagcgc gctatctggt gaaacactcc
    15181 accacgccga acggtattcc ggatgaccag gaagtcccga cggcgttcga aaagccggat
    15241 gtgggccgca tgatcacctc gctggtgctg attgaaacta tcgcgctgat tgctatctgc
    15301 ctgacggtgg ggaaaattgt tgcgcaactt ttggctggca ctgcttttga actgccgacc
    15361 ttcgtctgtg tactgtttgt tggcgtgatt ctgagcaacg gtctgtcaat gatgggcttt
    15421 taccgcgtct ttgagcgtgc ggtatccgtg ctgggtaacg taagcctgtc gttgttcctg
    15481 gcgatggcgc tgatggggct gaaactatgg gagctggctt cgctggcgct gccgatgctg
    15541 gcgattctgg tggtacagac catcttcatg gcgttgtatg ccatcttcgt tacctggcgc
    15601 atgatgggca aaaactacga tgcggcagtg ctggctgcgg gtcactgtgg ttttggcctc
    15661 ggtgcaacgc caacagcaat cgccaacatg caggcgatca ctgaacgttt tggcccgtcg
    15721 cacatggcct tcctggtagt gccgatggtc ggtgcgttct ttattgatat cgttaacgcg
    15781 ctggtgatta agctgtattt aatgttgccg atttttgccg gttaaccgat gaagcggcgg
    15841 tcttctcacc gccgcctttt acgcattcga gtaacgttcc gtttctggca tccagcgttc
    15901 tatcagggct tttgcctgct gtgggtaacg ttcgtgaata tggcgtgcca ggcgctgaac
    15961 ttccgggatc atcgcctgat cgcgcagtaa atccgccact ttaaattcag cattacccgt
    16021 ctgacgcgtg cctaacaatt cgccagggcc gcgaatctcc agatcttttt gcgcaatcac
    16081 aaacccgtcg ttgctgtcgc gcagtacttg cagacgaatt tgcgccgttt tagagagcgg
    16141 cgttttgtag agcagcacgc agtgagaagc caccgcacca cgtcccacgc gcccgcgaag
    16201 ctggtgcaac tgcgccagac ccagacgctc cgggttttcg ataatcatca ggctggcgtt
    16261 aggcacatca acgccgactt caataacggt agtggcaacc agcaggtgta actcgccttg
    16321 tttaaatgat gccatgacgg cctgcttctc ggctggtttc atccgtccgt gtaccagacc
    16381 aacattcaac tctggtagcg ccagtttcaa ctcttcccag gtagcttccg ccgcctgcgc
    16441 ttccagtaac tctgactctt caatcaacgt acaaacccag tatgcctgac gaccttcagt
    16501 tatgcaggcg tggcgcacgc ggtcaataat gtcggtacgg cgggtatcag gaatagcgac
    16561 cgtagtcact ggcgtgcggc ctggcggcag ctcatctatc accgaggtat cgagatcagc
    16621 atacgcagtc attgccagcg ttcgggggat cggcgtggcg gtcatgatca actgatgagg
    16681 atggaagcct tgctgctggc ctttctccca taacgccaga cgctgatgca cgccaaaacg
    16741 atgctgttcg tcgataatca ccagcgccag gccgttaaac tgcacctgtt cctggaagat
    16801 ggcgtgtgtt ccgacaatca tctgcacctg accgctggcg atggcttcct gctgtgccag
    16861 tcgcgcttta cctttctgct taccggcgag ccagcccact tcgataccga gcggtgcaaa
    16921 ccagttgcgg aagttattcg cgtgctgttc ggcgagtaat tcggttggtg ccatcaatgc
    16981 cacctgtttg ccgtgcgcaa tcgcacgcag cgcggcgagg gcagcgacca gcgttttacc
    17041 ggaacctaca tcgccctgta ccaggcgcat catcggcgca tccagcgcca tatcgcgctc
    17101 gatctccgcc accacgcgtg cctgtgcgcc cgttggcttg aacggtaagg ctgcgaggag
    17161 tttatttttc agcgcgtcat tggcgctaag cggctgggca tggaaacgtt gtgcaccagc
    17221 gcgtaacgcc aacatgctga ggttgtgcgc aagcagttct tccagaatca gacggcgttg
    17281 tgctggatgc tgcccggttt cgagatcgct aagctgtagc gtcggcggcg ggcggtgcaa
    17341 agtgcgcaac gcttccggta gcgtcatcat tccttgtgac agttccggcg gcaggagttc
    17401 ttcaatggcg caggtgtcga gcagatccag cgcctgatcg gttaatttac gcagcgtggc
    17461 ctgctttacg ccttccgttg ttgggtaaac cggcgttagc gtttcctgca actctggcgt
    17521 actgagatcg ccctgcacgc ggtattccgg gtggatcatc tccgcaccat atttaccgcg
    17581 ctttgcttcg ccataggcca gcacgcggcg gcctgtcgcc aggctatttt tcattgccgc
    17641 gttgaagttg aaaaagcgca tggtgagaat gccggaaccg tcgctgatct ggcaggtcat
    17701 catccgccga ccgccgaagg agatattgca gttcagcact tcgccttcca ccgtggcata
    17761 aacgcccggt agtagttctc cgatggggta gagatgggtg cgatcttcgt agcgcagggg
    17821 aaggtgtaag agtagatcct gcacggtatg caggttgatt ttcgccagct tgttactaag
    17881 cgctgcgcca acgcccgtta gggaactgag cgggacagca tctaacaggc gacctttcat
    17941 ggcacttacc ctgcagcctg catggtagcc caccagtcag catcggcttc aatttcgcct
    18001 tgctgattaa cgtgggggta aggcaggcct ttgcgttttg cgactttcgc cagcaccgga
    18061 tagccgcctt caaacagcag gcgttgttgc tctgcttccg gcaacatgct gttttcacgc
    18121 aggtacatgc ctgcattttg ccgctggcgc tgggcttcat aaagaatgag ggctgaggca
    18181 acggaaacat tcagcgactg caccatgccg atcatcggta tgatgatgtc ccgatccgcc
    18241 agggccaacg cttcctgcgt gatgcctgtt ttctcctgtc ccatcaaaat acaggtcggg
    18301 cgagtgtaat caatttcgcg gaaatcgaca gcgttatcag aaagatgggt tgccagaatc
    18361 tgcatgccct ggcctttcag atgagcgacg gcatcgccaa tggtgcggtg cgttttcacc
    18421 tgtacccagc tgttactacc cgcagctgcc gaagccatgg tgcgcatccg gctaccaggc
    18481 cagacagcat gaacttcatg tacaccaacg gcatctgcgg tacgaataat cgcagaaacg
    18541 ttatgaggtt tgtggacctg ctccatgcag acggtcagat caggctgccg cctggcgagc
    18601 atttcgcaga tgcgtgcata acgtgttggg ttcataaaac attaatttcg gtttcgggtg
    18661 actttaatca cgtctggcat cacgcggatt ttgcgcatga tattcgccag atgcacacgg
    18721 tcacgggcgg tcagacgaat aaaggcgctg tagacgcgac catctttctc ttccgtattc
    18781 aaactttgaa tattcgaggt ggtggtgtta attgccgctg tcaggtttgc cagcgcaccc
    18841 tgatgattga acatctccac cttgatttcg gtgatgaact cctgcgccgt ctctttatcc
    18901 cattccacag ccataaactt ctctggctct ttctggtagc cacggatgtt acggcaggat
    18961 tcatggtgga tcaccagacc tttaccgggg ctgacgtggg cgataatcgg gtcgccagga
    19021 atagggcggc agcatttcgc aaaggtgatc agcacgccat cggcaccttt aatgggcaga
    19081 tgtccgtgac tttgggttgc cggtggaatg gaggcgtccc catgttgcag atttttcgcg
    19141 accaccacgc tcattgcgtt accaaggccg atttcagcca gcagatcgtc aagcgttgcc
    19201 agcttcatgc ggtccagctc gcgctgaata ttttcctgcg ggatttcatt gagcttgcgg
    19261 ctgccaccca acgcatggtt gagcagacga cggcccaggc ttacagaatc atcacgcttg
    19321 aggtttttca gcaattgacg aattttggcg cgcgctttcg agctaacgac aaagttcagc
    19381 cacgcggcat tcgggcgagc gcccggtgcg gtaataattt caacggtttg accgctggta
    19441 agcggctgcg acagcgggta aggctggcgg tcgacgcgtg cgcccacgca ggcatgaccg
    19501 atatcggtat gcaccgcata agcgaagtcg acgggcgttg caccggcagg cagttcgaca
    19561 atgcgccctt ctggtgtgaa aacgtaaatc tcatccggga agagatcgga tttaacgctc
    19621 tcgataaatt caaacgagct accggcactc tgttgcagct ccagcaggct ttgcatccag
    19681 cgctgggcgc gtatttgcgc ggtagtgctg gtttcgccgt gctctttata agcccagtgc
    19741 gcggcaacac ccatctccgc catctgatcc atatcttcgg tacggatctg gacctcaacc
    19801 ggcacgccgt gcgggccaat catcgaggtg tgcaaagact gatagccgtt cgcttttgga
    19861 atggcgatat agtctttcac gcggcccgga cgcggcttgt acaggctgtg catctggccc
    19921 agcacgcgat aacaggtgtc agaatcattg acgatcacgc ggaaagcgta gatgtccatg
    19981 attgagtgaa aacgctgctc tttgagcacc attttgcagt aaatcgaata aagatgcttt
    20041 tcgcgaccac tgacgcggca cggtattccc gcttcctgca aacgcccttc gatttcagaa
    20101 agaatcttct ggatcatctc tttacggttg ccgcgcgcgg ctttcaccac ttctttgatt
    20161 acgcgataac ggttgggata cagcgcctca aaacccagct cttcgagttc ggttttaatg
    20221 tggtggatac ctaaacggtg cgccagcggg ctgtaaattt cgagggtttc acgggcgatg
    20281 cggcgacgtt tgtccgggcg aagtgagccc agcgtgcgca tgttgtgggt acggtcggca
    20341 agtttgatga gaatgacgcg gatatcctgc accatcgcca taatcatctt gcgaaagttt
    20401 tcggcctgcg cctctttctt atcgcggaac ttgagcttat caagtttcga caccccctct
    20461 accagctcgg cgacgctttt accaaaaagc tgttccatgt cctggtaggt ggcgggagta
    20521 tcttcaatca cgtcatgcag cagcgccgcc ataagcgttt catagtcgag tttcatctcg
    20581 gccagaatgc aggcaaccgc caccgggtgc gtgatatagg gttcaccgct tgaacgtgtt
    20641 tgcccctcgt gagcatcacg tgcaacgaga tacgcctgcc gcagacgctt gatttggtct
    20701 tccggcaggt aggtttgaat cagttgattc aggctttcaa acagatacaa gggcgacccg
    20761 ctttgtgatt aacgacgacc ttcagcaata gcggtaacgg cttgtaattc agcggcttcc
    20821 tgctcttgct gttcctggcg ttcgcgaacg tcgaggatct ggttgttgat cagaccttct
    20881 tcgatttcgc gcagcgcgat tacagtggtt ttatcgtttt cttccggtac cagcggatcc
    20941 tttccgccta cctgcatctg acgagcgcga cgcgcggcga ccagtaccag gtcaaaacgg
    21001 ttaccaattt tctctacagc gtcctgaaca gttacgcgtg ccatacttaa aaagctccac
    21061 aggtgaagaa atgactgggc atgatactga aatcaggttc agtctgccaa caatttgctg
    21121 attaaagcgt catgacgctg cttttggcgg ctcatgcgca gacgttcggc gcgaataatg
    21181 gtcttcaaat cggtcaacgc ggtatcgaag tcatcattca caatcaggta atcatattcg
    21241 gcgtaatggc tcatttctgc aacagcttgc gccatacgct ttgcaatgac ctcttcgctg
    21301 tcctgaccgc gaccgcgtag acggcggtcc agttcaattt tggacggcgg taaaataaag
    21361 atactccgcg cgtgcggcat cttctggcga atttgctgcg caccctgcca gtcgatatcg
    21421 agaaaaacat cgacaccggt cgccagtact tgctcaatgg cctcacgcga agtgccatag
    21481 taattaccaa aaacttctgc gtgttcgagg aacgcatctc tgctaatcat ttctttaaat
    21541 tcatcatgat taacaaagaa ataatgttca ccgtggacct caccaggacg cggttggcgt
    21601 gtggtgtgtg aaacagaaac ctgggtgtca tacaacggtt gggtttttaa taaagcctga
    21661 atcaggctgg atttacccgc gccactgggg gcagaaacaa tataaagcgt gccttgagcc
    21721 atgagtatct ttcgtatgtg attagcgaaa taaagcctac atacgagctt attatacacg
    21781 gcgcggccat gtgacgtagc ccttgctcac actttttgcg caaagttgtt gcgttttgcg
    21841 tagctgcttc cgcatttacc tgtcattcac tgcaataaag acgattctca ggaagcgtcc
    21901 tcgcagactc cgtttttgcc actcggcttt gctttcatca cgttgtatat ctgcctgact
    21961 atctacagcg gaggtgatga tgaaagtatg gatggcgata ttaataagta tcttgtgctg
    22021 gcaatcatcg gcgtgggcag tctgtccggc ctggtcgcca gccagagcac aggaagaaat
    22081 ttcccgcctg caacagcaaa taaaacagtg ggacgatgac tactggaaag aagggaaaag
    22141 tgaggtggaa gacggtattt acgatcagtt aagtgcccgt ctcacgcagt ggcaacgctg
    22201 ttttggtgaa gagacgcatc gcgacgctat gatgccgcct ttaaacggtg cggttataca
    22261 tccggttgca catacgggtg tacgcaaaat ggcggataaa atcgcattaa gtctgtggat
    22321 gcgcgaacgt agcgatcttt gggtgcagcc aaaagttgat ggcgttgcgg taaccctggt
    22381 ttatcgggac gggaaactga acaaagcaat cagtcgcggc aatggcctga aaggcgagga
    22441 ctggacgcag aaagttcgct taatttccgc tgtgccgcaa accgttagcg ggcctttagc
    22501 caacagtacg cttcaggggg aaatatttct caaacgcgag gggcatatcc aaaaacaaat
    22561 ggggggaata aatgcccgcg caaaagttgc tggtctgatg atgcgccagg acgatagcga
    22621 cacgctgaat tctctgggcg tttttgtctg ggcatggccg gatggaccgc agttaatgac
    22681 cgatcgttta aaagagctgg ctaccgctgg ttttactcta acgcagacgt atacccgtgc
    22741 ggtgaaaaat gctgatgaag ttgcgcgcgt acgcaatgcg tggtggaaaa cgaaattacc
    22801 cttcgtcacc gatggcgtgg ttgtgcgagc ggcgaaagaa cccgaatccc gccattggct
    22861 accaggccag gcagaatggc tggtggcctg gaaatatcaa cctgtagctc gggttgccga
    22921 agtgaaggcg attcagtttg cggtgggtaa gagcggtaaa atatcggtgg ttgcgtcact
    22981 cgcacctgtc atgcttgatg ataaaaaagt ccagcgggtg aatattggtt ccgtcaggcg
    23041 ctggcaggag tgggatattg cgcctggtga tcagatcctc gtcagccttg ccggtcaggg
    23101 tattcctcgc attgatgatg tggtgtggcg cggtgcagaa cgtacaaaac cgacaccgcc
    23161 agaaaaccgc tttaactcgt tgacctgcta ctttgcttct gatgtttgtc aggaacagtt
    23221 catttcacgc ttagtctggc tggggtcaaa acaggttctt gggctggatg gcattggtga
    23281 ggccggctgg cgctcgctgc atcagactca tcgctttgag catatctttt cctggctttt
    23341 attaacgcca gagcaattac agaacacgcc ggggatcgcg aaaagtaaaa gtgcgcagct
    23401 atggcatcag tttaatctgg ctcgtaagca gccttttact cgctgggtga tggcaatggg
    23461 aataccgcta acccgggcgg cgcttaatgc cagtgatgaa cggtcctggt cgcaactttt
    23521 attgagcacg gagcagttct ggcagcagct gccggggact ggatcggggc gtgtcagaca
    23581 ggttattgaa tggaaggaaa atgcgcaaat caagaagctg ggcagttggc tggctgccca
    23641 gcagatcaca ggttttgaac cttagtgtcc ttcgtggctg tagtaaaaga ccggtaatcc
    23701 cagcttaagg cgtagcgcca gtaagcgggc aaaaaagccg aataccaggg tggagataat
    23761 taccacatca tggttagaaa catagtgttg cagcgcgatg tacagcacgg cggaggcaaa
    23821 tgagacaccg gcatacaact ctttctggaa taccagcggg atgcgtttac agaacatatc
    23881 gcgcaaaacg ccgccaaata cgccggtcgt cacagctgcg acaacggcaa tgatcgggcc
    23941 gtgtcccata tccagtgcaa cctgtgcgcc aatgatagaa aagaccacca gtccgagcgc
    24001 atcgagcacc agaaacactt tgcgcaggta aggcattaca ggagcaacga tagtggtgag
    24061 tactgcggcg gtagcgacaa taatcacata ttccgggtgt ttgacccagc cgagcggata
    24121 gtggcccagc agaatatcgc gcactgaccc tccgccaatt gccgtggcgg tcgcaataat
    24181 aattacgcca aatgtatcca tgcgccgtcg tccggccgcc agcgcccccg tcatggcttc
    24241 ggcagtaatg ccaaccaaat acaaaatgtg taacagcatg gttctctccc taaaaatctg
    24301 tctggcaggg tagcgatttg tgcgtattgt cacgattgag attttttaag gtgagaggaa
    24361 attgataact tgtagttatg tgaaatcgct attttctgta gcgtagatgc attcttctga
    24421 cttcttgtct gagaaaatgc ataagaaaaa ataggttaaa attga
//
LOCUS       DAZGAP010000073        24126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGAP010000073 DAZGAP010000000
VERSION     DAZGAP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.073"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..2209
                     /gene="crfC"
                     /locus_tag="ACHLAM_002435"
                     /pseudo
     CDS             1..2209
                     /gene="crfC"
                     /locus_tag="ACHLAM_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
     gene            2206..3084
                     /gene="rdcB"
                     /locus_tag="ACHLAM_002436"
     CDS             2206..3084
                     /gene="rdcB"
                     /locus_tag="ACHLAM_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO7853951.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDAFRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            3348..4850
                     /gene="proP"
                     /locus_tag="ACHLAM_002437"
     CDS             3348..4850
                     /gene="proP"
                     /locus_tag="ACHLAM_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO7853952.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4962..5051
                     /gene="pmrR"
                     /locus_tag="ACHLAM_002438"
     CDS             4962..5051
                     /gene="pmrR"
                     /locus_tag="ACHLAM_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO7853953.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5027..6127)
                     /gene="pmrB"
                     /locus_tag="ACHLAM_002439"
     CDS             complement(5027..6127)
                     /gene="pmrB"
                     /locus_tag="ACHLAM_002439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO7853954.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6128..6796)
                     /gene="basR"
                     /locus_tag="ACHLAM_002440"
     CDS             complement(6128..6796)
                     /gene="basR"
                     /locus_tag="ACHLAM_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO7853955.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6793..8436)
                     /gene="eptA"
                     /locus_tag="ACHLAM_002441"
     CDS             complement(6793..8436)
                     /gene="eptA"
                     /locus_tag="ACHLAM_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO7853956.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8540..9877)
                     /gene="adiC"
                     /locus_tag="ACHLAM_002442"
     CDS             complement(8540..9877)
                     /gene="adiC"
                     /locus_tag="ACHLAM_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO7853957.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10014..10775)
                     /gene="adiY"
                     /locus_tag="ACHLAM_002443"
     CDS             complement(10014..10775)
                     /gene="adiY"
                     /locus_tag="ACHLAM_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO7853958.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11111..13378)
                     /gene="adiA"
                     /locus_tag="ACHLAM_002444"
     CDS             complement(11111..13378)
                     /gene="adiA"
                     /locus_tag="ACHLAM_002444"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO7853959.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13577..14485)
                     /gene="melR"
                     /locus_tag="ACHLAM_002445"
     CDS             complement(13577..14485)
                     /gene="melR"
                     /locus_tag="ACHLAM_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO7853960.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14768..16123
                     /gene="melA"
                     /locus_tag="ACHLAM_002446"
     CDS             14768..16123
                     /gene="melA"
                     /locus_tag="ACHLAM_002446"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO7853961.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(16120..16770)
                     /gene="yjdF"
                     /locus_tag="ACHLAM_002447"
     CDS             complement(16120..16770)
                     /gene="yjdF"
                     /locus_tag="ACHLAM_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO7853962.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(16893..18539)
                     /gene="fumB"
                     /locus_tag="ACHLAM_002448"
     CDS             complement(16893..18539)
                     /gene="fumB"
                     /locus_tag="ACHLAM_002448"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7853963.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(18617..19957)
                     /gene="dcuB"
                     /locus_tag="ACHLAM_002449"
     CDS             complement(18617..19957)
                     /gene="dcuB"
                     /locus_tag="ACHLAM_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO7853964.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(20528..21247)
                     /gene="dcuR"
                     /locus_tag="ACHLAM_002450"
     CDS             complement(20528..21247)
                     /gene="dcuR"
                     /locus_tag="ACHLAM_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO7853965.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(21244..22875)
                     /gene="dcuS"
                     /locus_tag="ACHLAM_002451"
     CDS             complement(21244..22875)
                     /gene="dcuS"
                     /locus_tag="ACHLAM_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO7853966.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            23055..23285
                     /gene="yjdI"
                     /locus_tag="ACHLAM_002452"
     CDS             23055..23285
                     /gene="yjdI"
                     /locus_tag="ACHLAM_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO7853967.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            23297..23569
                     /gene="yjdJ"
                     /locus_tag="ACHLAM_002453"
     CDS             23297..23569
                     /gene="yjdJ"
                     /locus_tag="ACHLAM_002453"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7853968.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            23796..24092
                     /gene="ghoS"
                     /locus_tag="ACHLAM_002454"
     CDS             23796..24092
                     /gene="ghoS"
                     /locus_tag="ACHLAM_002454"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7853969.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
BASE COUNT         5888 a         5851 c         6292 g         6095 t
ORIGIN      
        1 gagttaagtc aggaggctga acgcctgtta cagctttctc gccaacagtt gcagttactg
       61 gaaaaaatac ctctctctgt acccggagac gatacgccac aactggcttt accctggagt
      121 cagcctaata tcgccgaacg tcacgcgatg ctgaataatg agttgcgtaa aatttcccgg
      181 ctggaaatgg tgctggcgat tgtcggtacc atgaaagcag ggaaatcaac caccattaat
      241 gccattgttg gtacggaggt tctgcctaat cgtaatcgcc caatgactgc gctgccaacg
      301 cttattcgcc atacgcccgg gcaaaaagaa ccggtactgc atttttcaca tgtcgcgcca
      361 atcgaccatt taattcaaca attacaacag cgcctgcgtg attgcgatat taagcatctg
      421 accgatgtgc tggaaataga taaagatatg cgtgcgctta tgcagcggat tgaaaatggc
      481 atcgctttcg aaaaatatta tctgggtgct caacctattt ttcattgtct gaaaagtttg
      541 aatgatttgg tgcgactggc gaaggcgctg gacgtcgatt ttcctttttc tgcttacgcc
      601 gccattgagc atattcccgt gattgaagtg gagtttgtcc atctggcggg gctggagagt
      661 tatcccggtc agttgacgtt actggatacc cccgggccaa atgaagccgg gcaaccgcat
      721 ctgcaaaaaa tgcttaacca gcagctggca cgcgcctcgg cggtactggc ggtgctggat
      781 tatacgcaac tgaaatcgat ctccgatgaa gaggtccgtg aggcaatttt ggcggtgggg
      841 caatcggtgc cgttgtatgt gctggtcaat aagttcgatc aacaggatcg taacagtgac
      901 gacgccgacc aggtgcgggc actgatttcc gggacgctga tgaaaggttg tattacgcca
      961 cagcagatat ttccggtgtc gtcgatgtgg ggctacctgg cgaatcgggt gcgccatgag
     1021 ttagccaaca acggtaagtt accaccgcca gagcaacaac gctgggtgga agattttgcc
     1081 catgccgcgc tcggcaggcg ctggcgtcat gccgatctgg cggacctcga acatattcgt
     1141 catgctgccg atcagttgtg ggaagattcg ctgttcgccc agccaattca ggcgttgctt
     1201 catgccgctt acgctaacgc ctcgttgtat gctctgcgat ctgccgcgca taaactgttg
     1261 aattacgcgc agcaggcgcg ggaatacctg gattttcgtg cgcacgggtt aaacgtcgct
     1321 tgtgaacaat tgcggcaaaa tatccatcag atcgaagaaa gtttgcagct attgcaactc
     1381 aatcaggcgc aggtgagcgg cgagattaaa catgaaatcg agctggccct gacctccgcc
     1441 aaccactttc tgcgtcaaca gcaagatgcg ctgaatgcgc agttagccgc cttgtttcag
     1501 gatgattcag ggtcattaag cgagattcgt acctgctgtg agacactgtt acagatggcg
     1561 cagaacacca ttagtcgcga ctttacgctg cgttttgccg agctggaatc caccctttgc
     1621 ccgggtgtta accgatgtta ttcgccccat tgagcaacaa atcaaaatgg aattgagcga
     1681 gtcagggttt cgtcctgggt ttcattttcc tgtttttcac ggcgtagttc cccacttcaa
     1741 cactcgccag ctgttcagtg aagtcatttc gcgccaggac gcaatggacg agcagagcac
     1801 gcgtttaggc gttgtgcgtg agactttttc gcgctggttg aatcagcccg actggggacg
     1861 gggaaatgag aaatccccga tagagacggt tgattacagt gcgttgcaac gagcattaag
     1921 cgcagaagtc gatctttatt gccaacaaat ggctaaagtt ctggcagagc aggtcgatga
     1981 atctgttacg gcaggcatga atactttttt cgctgagttc gcttcatgtt tgacggaatt
     2041 acagacgcgt ttacgcgaaa gtctggctct gcgtcaacaa aatgaatcgg tggtcaggct
     2101 gatgcagcag caattgcagc agactgtgat gactcatggc tggatttaca ccgacgctca
     2161 gctgttacgc gatgatattc aaacactttt cacggcagaa cgatattgac caagacgtta
     2221 ctggacggcc ccggtcgcgt gctggagtcg attcatcccc gctttttagt ggatctggcg
     2281 cagggcgatg atgcccgcca tccacaagcc catcagcagc agtttcgtga acgactgatg
     2341 caggaacttc ttgcgcgtgt gcagcttcag gcatggacga acggcgggat gttaaatgcg
     2401 ccgcttagcc tgcgtctgac attggtggaa aaactggcgt cgatgctgga tcccggtcat
     2461 ctggcgctga cgcagatcgc gcaacatctg gcgctgctgc aaaaaatgga tcaccgccag
     2521 cactctgctt tccctgagct cccccagcaa attgccgcct tgtatgagtg gttttcagcc
     2581 cgttgtcgct ggaaggaaaa ggcgttaacg caacgaggcc tactggtgca ggcgggtgaa
     2641 cagagcgagc aaatttttac ccgctggcgt gctggggcgt acaacgcctg gtcgttgcct
     2701 gggcgctgtt ttatcgttct ggaggagttg cgttgggggg catttggcga tgcctttcgt
     2761 ctgggaagcc cgcaagcggt ggcgttgttg ctgggtgatt tgcgcgtgaa agcgacacaa
     2821 catctggcag agagtatcaa tgcggccccg accacgcgtc actattacca tcagtggttt
     2881 gcctcttcga ccattccgac gggcggggat catgctgatt ttttaagttg gctgggaaag
     2941 tggtccacgg cagataaaca acccgtttgc tggtcagtga cccaacgctg gcgaactgtc
     3001 gcactgggga tgccacgact ctgttcagcg cagcgtctgg cgggggcaat ggtcgaggaa
     3061 atcttctctg taaatttggt gtaaataatc agttacatca atgagtccta aacgaaatcc
     3121 atgtgtgaag ttgatcacaa atttaaacgc tggtagggta aaaaggtcat taactgccca
     3181 attcaggcgt caactggttt gattgtacat tccttaaccg gagagtgtaa gcaaacccgc
     3241 tacgcttgtt acagagattg catcctgcaa ttcccgctcc ccttttgcgg ccgtcgcgct
     3301 gatttttctg gcgtttgcgg aaatgggcca actctgcgag gaaagctatg ctgaaaagga
     3361 aaaaagtaaa accaattacc cttcgtgatg tcaccattat tgatgacggt aaactgcgta
     3421 aagccattac cgcagcatca ctgggtaatg caatggaatg gtttgatttt ggtgtttatg
     3481 gttttgttgc ttacgcatta ggtaaagttt ttttcccggg ggctgacccc agcgtgcaga
     3541 tggttgctgc acttgccact ttctccgttc cctttctgat tcgaccgctt ggtggactct
     3601 tctttggtat gttgggcgat aaatatggtc gccagaagat cctcgctatc actattgtga
     3661 ttatgtcgat cagtacgttc tgtattggct taataccgtc ctacgacacg attggtattt
     3721 gggcaccgat tctgctgttg atctgtaaga tggcacaagg tttctcggtc ggcggtgaat
     3781 ataccggggc gtcgatattt gttgcggaat actcccctga ccgtaaacgt ggctttatgg
     3841 gcagctggct ggacttcggt tctattgccg ggtttgtgct gggtgcgggc gtggtggtgt
     3901 taatttcgac cattgtcggc gaagcgaact tcctcgactg gggctggcgt attccgttct
     3961 ttattgctct gccgttaggg attatcgggc tttacctgcg ccatgcgctg gaagagaccc
     4021 cggcgttcca gcagcatgtt gataaactgg agcagggcga ccgcgaaggt ttgcaggatg
     4081 gcccgaaagt ctcgtttaaa gagattgcca ccaaacactg gcgcagcctg ttgacatgta
     4141 ttggtctggt aattgccacc aacgtgactt actacatgtt gctgacctat atgccgagtt
     4201 atttgtcgca taacctgcat tattccgaag accacggggt gctgattatt atcgccatta
     4261 tgatcggtat gctgtttgtc cagccggtga tgggcttgct gagtgaccgt tttggccgtc
     4321 gtccgtttgt gctacttggt agtgttgcac tgtttgtgtt ggcgatcccg gcgtttattc
     4381 tgattaacag taacgtcatc ggcctgattt ttgccgggtt actgatgctg gcggtgatcc
     4441 ttaactgctt tacgggcgtt atggcttcta ccttgccagc gatgtttccg acgcatatcc
     4501 gttacagcgc gctggcggcg gcatttaata tttcggtgct ggttgccggt ctgacgccaa
     4561 cactggcggc ctggctggtc gaaagctcgc agaatctgat gatgcctgcc tattacctga
     4621 tggtagtggc ggtggttggt ttaatcaccg gcgtaaccat gaaagagacg gcaaatcgtc
     4681 cgttgaaagg tgcgacaccg gcggcgtcag atatacagga agcgaaggaa attctcgtcg
     4741 agcattacga taatatcgag cagaaaatcg atgatattga ccacgagatt gccgatttgc
     4801 aggcgaaacg tactcgcctg gtgcagcaac atccgcgaat tgatgaataa gctgaaacgg
     4861 atggcctgat gtgacgctgt cttatcaggc caattgaact cttaaggttc acttaatctc
     4921 tgacgcgcat actctcctcc aggttaacgg aggagagtgc aatgaaaaac cgtgtttatg
     4981 aaagtttaac taccgtgttc agcgtgctgg tggtcagcag ctttctttat atctggtttg
     5041 ccacgttctg atctttcttc agccgtaccc aggcccgcgt gccggaagtc tcttgccggt
     5101 tttgcaggaa aaactgcccg tgatgcaact gtgtaatgcg gctgacaata cttaacccca
     5161 gaccaatccc gccataacgg ctgtccatac gtacaaacgc tttactcaac tccccgcatt
     5221 tactctcatc aatacccggt ccttcatctt caacggccat gaccgctccg ccatcttctt
     5281 gcagcttaat cataatgttg ctgccttgcg ggctgtaacg atgggcgttt tctaccaggt
     5341 ttcgcaataa catccgcagc agggttgcat caccctgaac ggtgatgtcg gcggcgctct
     5401 ctggcaatag caggttttgc tgtcgctggt cgagcatggt actgagttcg tcatacgagg
     5461 ggagaatgac atcttccagc agttttacat gttgataatt accggaagaa aatgactgtc
     5521 cggcacgcgc cagttgcagc agctgggaga cactctccat catctgatca agtcgagcca
     5581 ctaacggtgc tacatcaatg tgatgcgttt tcgccagcag ttccagatgc aaacgcaccc
     5641 ccgccagtgg cgttcgcagt tcgtgcgcga cgtcagcggt aaacaacctt tcgttatcca
     5701 gcgtgctggt caggcgactg accagatcgt tcaacgccga aaccaccgct tcgatttcga
     5761 gggtggcgct gtgaatggca ataggcgtta agttgtcggc ggtgcgcgct tccagctctt
     5821 tttgcagctc cgccagcggc cgggtgatgc ggcgtaccgc ctgatagcag ataaatagcg
     5881 tcaggctgac cataaagacg ccggggacga tcaggctggc gaccgcctcg cggatctcac
     5941 gcatgatgtg gcgatcgttg ttgcgattgt cgcgtagcgc ctgctcaaac agctgaatct
     6001 gctcggtact ttcatgccat agccagaaga cgctgatcag ctcaaacacc aacaaaatgg
     6061 ccccaatggt caatatcagc cgttggcgca gcgatattgg tcggcgcaga aaacgcatca
     6121 gattcaatta gttttcctca tttgcgacca gcatatagcc aaagccgcgc acggtgcgga
     6181 tacgggcttt gcccactttg tcgcgcagat tgtggatatg cacttccagg gtgttggtcg
     6241 agggttcatt gtcccagtta tagatgtcgt tgtagagaat ttcccgatgc accggactgc
     6301 ctgctttgag cattaaccgt gacagcagag catattcttt gggcgtcaga atcaactctt
     6361 caccgcccat ccatacctga cggcgaccca tattcagcgt cagattgcca acaatcagct
     6421 cactttcgcc ctgattatta tggcgtcgta gcagggcgcg gatacgggcg tgtaactctt
     6481 ccagcgcaaa aggcttcacc agatagtcat cggcaccgac atccagcccg gcgattttgt
     6541 cggtcagcgt atcgcgagcg gtgaggatca gtaccggcag ggtgtatttt ttctgccgga
     6601 tacgggcgag aaaatgcagt ccatcttcgt cgggtaaccc taaatccagt accaccaggc
     6661 tgtaatgacc ggcctcaagg ctttgttccg ccatccgcgc ggttgtcacg ccatcgcacg
     6721 cgtagccttc ggtttgcgcc gccagaatta atccctgcaa taacagcgta tcgtcttcaa
     6781 caatcagaat tttcattcac tcactctcct gcaagtttgc agaatatcat ccgcagcctg
     6841 gtaatacttc gtctcaacgc cagttaatcc caatagcgtg gagaataaat tatcttgtga
     6901 atagtgttgc gtttgcgcct gtttttgcag gcagttctgg tcaacctgat accgtttttg
     6961 ataatcctcc gacagccaca gcagcatcgg cacctgtttt tggctatccg gggcgatggc
     7021 ataaggcaga ccgtgcagat agatgccatt ttcacctaac gactcaccgt ggtcagaaag
     7081 ataaaccagg ctggtggtaa atttatcctg atgttctttc agcagattaa tcgctttatc
     7141 aacaatatag tcgacgtaaa ccagcgtgtt gtcgtaagtg ttcaccagtt gctctttggt
     7201 acaggtctgg atctcgttag tgtcacaggt tggggtaaat ttcctgaact gcggcggata
     7261 gcggttgtaa taggtcggac cgtggctgcc gatggtgtgt aagacaatca cgccatcacc
     7321 ctgcaggtta ttgatgtact cttccagccc gtggaacagc acttcgtcat agcattcgcc
     7381 gttgatgcac tgacctggca gattcagcgc ggtgacgttc tggtgaggta cgcgatcgca
     7441 aacgccttta cagccgccat cgttgtcatt ccacagcacg ttgatgcccg ctcgctgaat
     7501 gatatccagc acgccttcct ggtgctgtgc cagctcttct ttgtagtgct cacgcggcat
     7561 atccgagaac atgcatggta ctgaaactgc cgttgccgtg ccgcaagagg cggtattagg
     7621 gaaatagacc acgttatctt ttgccagacg aggattggtt tcgcgtggat aaccattcag
     7681 cgagaaattc tctgcgcgcg acgtttctcc gacgatcaaa atggtcaggt ttttacgttt
     7741 cccattttgc attaatgggt tgcggtgagc atcttcacca atgcgtatca gtggcaggtt
     7801 ggctgaccgc tggtgagagt accacgacca gctagcaaca atgctgttgg acgggcttaa
     7861 ggatttcacc agctctttgt tgttgcgaaa cagcgaggca tagtctttgt agaacagtgc
     7921 ggcaaccagc agaatcagca gtacagaaat caggatgttg gccccgcgga aaagaacgct
     7981 gcgcaggcgt gaggtggtgg gggtgatttt gatccagcag gcaataatcg cggctaacac
     8041 gccacttaac cccagcgtta ataacattcg tggcgtcatc agggcataac tttccgccgg
     8101 cgtagtatca atgatattgg tgatcattga acggtcgatg acgatgccat aggtcatgat
     8161 gaaatattgt gcagccgcac cgaccagaat aaacaggcag gcgagtggac gattaagcca
     8221 taagaaagag cctagtgtca ggacaatatt aatcacgctg aatgcgacga ccggcatcga
     8281 caagaaaacc agcacgttat gcagtgaatc cagcggtaac acctgtagca cctgtttaaa
     8341 aaaggcgata ttcaggcaga ctgagatata aaaagtagca agtaataacc aggagaataa
     8401 atttaaagag ggtcttttta gtaggcgctt caacatcacg gtgtttccat cgaacaaagt
     8461 gcgcatatgc tcgcaaagca aaattaagcc aaccttaagt tcttaaggtt ggcttttatg
     8521 tttgcttgat taaggggaat taatctttgc ttattggtgc atctaaggga tacgggtttt
     8581 tatgcagccg gttgtaattc agcgcataca tggcggtgat taccatcaga gtgacaaatg
     8641 accacataac ctctttcgcg ccggacccta ccacggccca gatgcagtag aggaaggcaa
     8701 tggtagtcac tgccaggtat gccgggcgtg ctttaccaaa gtgaccgtgg ccgagcagta
     8761 gtaacgccgc acaggtgtaa agatatggca ccagtgtaaa gatgaccgac acggaagaaa
     8821 ccagaccgaa ttctttggtc gcgtttggcg aaatgctact gagctggaag atggtcatca
     8881 aaataccgac gataatcagc cccgccactg gcgtgcccgc tttattaaca cgggcaaaaa
     8941 tcggtgggaa cagtccgtca tcggcagcgg ctttcgccgt ttgacccgcc agcaacgtcc
     9001 agccgcccag tgaacctaag caacccgcag ctgcgcagaa ggaaacaatg gccccggcgg
     9061 tgtcacccaa cgccatccgt gcggcatcac cgaatggcga agcagaaacg cgcagtgcgg
     9121 cattagggat catccccata atcgcggtgg tagaaagtac atagcaaacg gcagcaatca
     9181 ataccccgcc aatggtggcg atagggacgt tgcgtttcgg gtttttcacc acacctgcgg
     9241 caacggaggc actttccaca ccgatgaacg accacagcgt aacgttaagg gtactttgaa
     9301 tcgcaccgaa ggtgcccaga ccgctgacgt tccatgccgc catataggtt tcaccacgga
     9361 accagaacca gccaaatacg gcaatcccga cgatggggat cagcgccagc acggtagcaa
     9421 cggcctgtac gcgggtgatc attttcggac cgacaatgtt cagcaggacg aagatccaca
     9481 gcaccacgac gcaggtgatg gttaatacca atgggtcttt cagaatcggg aagaagtaac
     9541 ttaaatatcc tacgccaatg accaccatgg cgatattgcc gatccagcag gccagccagt
     9601 agaggacgtt ggtttgataa ccgagaaacg ggccaaagca gcggcgggcg taagcgtaag
     9661 aaccaccagg acttgggtcg aggaacgaca ttttggcgta taccatcgag agccccagcg
     9721 caccgataat cgtcaccaac catccataaa tggcaatccc gccagtggag gccaggtttg
     9781 caggtaacag aaaaacacct gaccccataa tattccccga caccatcagg gtgacgggga
     9841 ttaagcccac tttgtgagca tcagcatccg aagacataat taaactcctg cgaaggcgag
     9901 cttcgtgaca ataaattacg tcgtatcata cgcctgcatt cgatagttga attattatcg
     9961 gccgggttat cgctgatgcc gttattaata tagctcttcg ttaaggttaa aaatcaggcg
    10021 acagtgcgtt ctctgtgctg actaacataa tgcagcggcg tcataccgta gaagtcttta
    10081 aagacagaaa taaagtacga cgtactgttg tagccgcagg actgtgatac ctgagagata
    10141 tttttaccgt ccatcattaa ttcatttacg gcatagcgca tgcggcaggt ggtgattatt
    10201 tggctataac tggtgttttc gcttttcagc tttttcttta acagacttgg gctaagacat
    10261 aaacaactgg ctaccatact aagattccag tctttgtgga tatcgctttc aataatttga
    10321 taaacgctgt cacttacaca attacgtaac atatacatca ttagtgaaag gaatttttta
    10381 ctgtcgagaa aacgggatag cacagtgaat aataatgcac gtgttcgttc cttttctgac
    10441 tcagtttcag cgggcatcat gctatgttgc gcggcaacgc gaaagacgtc tggcgtcagg
    10501 catggaaggg tgaggatggg cgtagcgcta cgttgccata ctggtatttg cgagagatct
    10561 ttattcagaa accggaggta atctttgatg atgtcgtgac taatatgagc aaccaaagtg
    10621 ttattaagag aggagacgtc gataatatta ttttcacaat ttaataacgc catatggtta
    10681 gctttaaggc taataggttc tttcccattc acccttatcc agacatcttt ttcagtcaat
    10741 aaaacaatac aaggttggtc gctgcaaatc ctcatacatg tactcctgag tgcgaataaa
    10801 agaccagttc tcgctaaagc aaagcgatac cgggaaatgg ttatataatt gtatgatgaa
    10861 tataaacatg cataaaaata acgagtttta ctaatattac aaatttttat agtaatttct
    10921 catgtacata tttttttaca ggctaaaaaa aggataaata acaggcaaaa aaaagcgcgg
    10981 ggtgggaccg cgcaaaaagc atgacatttt ttgtttgcag caatttttat gaaaacgttg
    11041 ccatacttaa aaggcctcaa gccaggcggc gtaatgttat ttaacaatta cgccttcagc
    11101 ggaatagtgg ttacgctttc acacacataa cgtggtaaat accgtcaata atttcagtcc
    11161 cttcagtttc gtgttcaaat ccagggaaat gatggtccca ggattgcagc gagcgtaaat
    11221 aacttacttg cggactgttt ttatcgccga agttttcacc agacagcagc atcgggattc
    11281 ctggcggata cgggataact gagtttgccg cgatgcgtcc tggtaggttt tcaatggata
    11341 ccagttcgac attgttgtcg acaatcgcgt tgtacgcttc tcgcggggtg atttccgcca
    11401 ccggcaggcc ggaataggct tcgttcaacc gtgcgccagg gttgttttct ttcagccagg
    11461 caaacatggt gtcacccaga tcgtgaatcc ccatgttcgc gtaagtgtca ggatattgtt
    11521 caacaagttc cggcatcacc tgcgccagcg gggtgttggc gtcatagtgg cgtttgaagg
    11581 agcaaagggt gttaaccaga gtcccccatt tcccacgggt cacgcccata gagaacagga
    11641 acataatctg gaagtcagtg gtacgggtag gcacaatgcc gtggcgacca agccaggctg
    11701 tgaccagcgc cgccggaaca ccggtttctt ccagttcacc atcttcaccc attcccggag
    11761 caaggatgct gactttaatc gggtcgagca tactccagtt atccggaata tctttgaagc
    11821 cgtgccagct ttcgcccgga tgcatgaccc agcagtcctg aacggtggtc agcagtttgg
    11881 ttggtgcgtc agcaaagtca taggttttgc cggtttgtgg gtcggtgacg acttctttgt
    11941 tccacggttt gaagaaccag ctaccgtcag cggtgaactc tttatacagc cgcgccatcg
    12001 cctgacggaa atcaaccgct tcgtcgatca cttcctgcgt cagtgacagg ccgctgttgc
    12061 cgtccatcat cgacaccgcc acgtcgttgg atgcgcagat ggcatacagc ggggaggtgg
    12121 tggcatgcat catgtaggcc tggttgaagc gggagaagtt aatcgcccca cgaccttcac
    12181 gtacatgaat ataagaggcc tgtgacagcg cattcagcag tttgtgggtg gagtgggtgg
    12241 cgaaaacggt agggccgttg tgatcgccag gttcgccgcg catggcatag tgatcggcat
    12301 agattgggtt gaaacgtgca tagccgtacc aggcttcgtc aaagtgcaga cgatcggagg
    12361 ttttttccag cagatcctgc gcttctttag cgttataaca cacgccgtca taggtacagt
    12421 tggtcaccac gcagtaagac ggtttttgcc cggctttgtc tttggtcagc gggctttcac
    12481 tgattttctt ctgcaaggtt tcaggttgca tttcctgcgg atagattggc ccgataatgc
    12541 cgtagcggtt gcggcttggc accatgtaga ccggtttcgc gcctgtcagc atcaagcctt
    12601 gttcgatgga tttatggcag ttacggtcaa cgaccacgac atcgttatcg gtcatgcaag
    12661 cctgcatgat ggtgcggtta gagccggaag taccgacgac taccgaccag gagcgatcgg
    12721 caccaaatac gcgtgcagca tatttttcgc tttcgccaaa tgcgccagta tggtcaagca
    12781 aagaaccgag ggaagttcgt tcgatgccca tgtcggtgcg gaacagattt tcaccatagt
    12841 agtcatggta gaaacgtccg gcgggtgttt tggtaaaacc aacgccgccc tggtggcctg
    12901 gcgctgccca ggaatattca tggatgtcgc tatatttcat cagcgcgctg aacagtggcg
    12961 gcaacagttg ctggcggtag cgggtcatcg cggcaacggc gcgtccggcg ataaagtcgg
    13021 cggtatcttc cagaatccag gcgaattcat cgacaagctc cagcagatcc cggtccattg
    13081 cggcgagggc tttttcccga tcgcccaaca ggaagaccgg cacgttttgt tgacgctcat
    13141 gaagcttacc gatcaattgt ctgacgtttt gatgttcgtc cggatgttcc atttgatagc
    13201 tgaacatcag gcagtcaatg gcttcgtttg aagagagaat ggcaaaacca tcatcaaagg
    13261 aggtggattt aatcacggta acattttgct ggcttaaagc atctgccaga cgctcaacgg
    13321 cgttaccgac ccaggtgtct tgatggagaa actcgctttc aacaattaat actttcatca
    13381 ttgcttaccc ggttatgaag gaatcttcgt tgcgggcaag tatcttccgg ccttttcggg
    13441 ctgtaaagcg cgtgaaaaac aaacattttt gaaatatttg tttttataaa caatatgtta
    13501 tgtgattatt ttaaattatt ttcactgctt attatcgacg gctaaactat ttttttggct
    13561 gatactgata tcgtctttag ccgggaaacg tctggcggcg ctgttggcta agtttgcggt
    13621 attgttgcgg tgacatgccg acatatttgc cgaacgtgct gtaaaaacgg ctactcgaac
    13681 gaaagcctgc cgtcagggca atatcgagaa tacttttatc ggtatcgctc agtaacgcgc
    13741 gaacgtggtt gatgcgcatc gcggtaatgt actgtttcat cgtcaattgc atgacccgct
    13801 ggaatatccc cattgcatag ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg
    13861 tcagtgcctg atcatagttt tcggcaataa agcccagcat ctggctgaca taaaattgcg
    13921 catggcgcga gacgctgttt ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc
    13981 agccagagag gctaaatcgc ttgagcatca ggccaatttc atcaatggcg agctggcgaa
    14041 tttgctcgtt cggactgttc aattcctgct gccagcggcg cacttcaaac gggctaagtt
    14101 gctgtgtcgc cagtgatttg atcaccatgc cgtgagtaac gtggttaatc aggtctttat
    14161 ccagcggcca ggagagaaac agatgcatcg gcagattaaa aatcgccatg ctctgacagg
    14221 ttccggtatc tgttagttgg tgcggtgtac aggcccagaa cagcgtgata tgaccctgat
    14281 tgatattcac tttttcattg ttgatcaggt attccacatc gccatcgaaa ggcacattca
    14341 cttcgacctg accatgccag tggctggtgg gcatgatatg cggtgcgcga aactcaatct
    14401 ccatccgctg gtattccgaa tatagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc
    14461 tgcacataaa cgtatctgta ttcatggatg gctctctttc ctggaatatc agaattatgg
    14521 caggagtgag ggaggatgac tgcgagtggg agcacggttt tcaccctctt cccacagcgg
    14581 cgaggggagc gtccgagcat catgaggccg aaaactctgc ttttcaggta acttattccc
    14641 ataaactcag atttactgct gcttcacgca ggatctgagt ttatgggaat gctcaacctg
    14701 gaagacggag gttttctgca gattcgcctg ccatgatgaa gttattcaag caagccagga
    14761 gatctgcatg atgtctgcac ccaaaattac atttatcggc gctggttcga cgattttcgt
    14821 taaaaatatt cttggtgatg tgttccatcg cgaggcgctg aaaacggcgc atattgccct
    14881 gatggacatt gatcccaccc gcctggaaga gtcgcatatt gtggtgcgca agctgatgga
    14941 ttcagcaggg gccagcggca aaatcacctg ccacacccaa cagaaagaag ccttacagga
    15001 tgccgatttt gtcgtggtgg catttcagat tggcggttat gaaccttgca cggtgactga
    15061 tttcgaggtc tgtaagcggc atggtctgga acaaaccatt gccgatacgt tggggccggg
    15121 cggtattatg cgcgcgctac gtaccattcc gcatctgtgg caaatttgcg aggacatgac
    15181 ggaagtctgc cccgatgcca ccatgctcaa ctacgttaac ccaatggcga tgaatacctg
    15241 ggcgatgtat gcccgctatc cgcatatcaa acaggtaggg ctgtgccatt cggtgcaggg
    15301 aacggcggaa gagctggcgc gtgacctcaa tatcgacccg gctacgctgc gttaccgttg
    15361 cgcaggtatc aaccatatgg cgttttacct ggaactggag cgcaaaaccg ccgacggcag
    15421 ttacgtgaat ctctacccgg aactgctggc ggcttatgaa gcagggcaag caccgaagcc
    15481 gaatattcat ggcaatactc gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct
    15541 gggctacttc gtcacggaat cgtcagaaca ttttgctgag tacacaccgt ggtttattaa
    15601 gccaggtcgt gaggatttga ttgagcgtta taaagtaccg ctggatgagt acccgaaacg
    15661 ctgcgtcgag cagctggcga actggcataa agagctggag gagtataaaa acgcctcccg
    15721 gattgatatt aaaccgtcac gggaatatgc cagcacaatc atgaacgcta tctggactgg
    15781 cgagccgagt gtgatttacg gcaacgtccg taacgatgga ttgattgata acctgccaca
    15841 aggatgttgc gtggaagtag cctgtctggt tgatgctaat ggcattcagc cgaccaaagt
    15901 cggtacgtta ccttcgcatc tggccgccct gatgcaaacc aacatcaacg tacagacgct
    15961 gctgaccgaa gccattctta cggaaaatcg cgaccgtgtt taccacgctg cgatgatgga
    16021 cccgcatact gccgccgtgc tgggcattga tgaaatatat gctcttgttg acgacctgat
    16081 tgccgcccac ggcgactggc tgccaggctg gttgcaccgt taaggggtaa tcgcttccgg
    16141 cgctctggtg attaagccaa agcgccgtag ctggcggcaa tgaaagcgag cgaggaatat
    16201 caccgtcgtt aatgcgccaa gcagcgcgca gaacatatcg gattgcgtgt cccactggtc
    16261 gccctgggtg ccgagaaaat catccgctcc ctgacccatt gccagcgccg cccaccactc
    16321 gattaattca tacatcgcgc ttatcgccag cgccacgcag cagaccagaa acgccaccat
    16381 cttacgtccg cgaacgtaca tcccgcgtac aaggatctct cgtgccacca gtgcaggcac
    16441 cagcccctgg aaaaaatgcc ccagtttgtc atacggatta cggctcaacc ccagccattc
    16501 ctgcacctca aaaccaacgg ggactttcgc gtaggtgtat tgcccgccga ccatcaggat
    16561 gatggcgtga aagaaaatga gcgtataaag cagcggagtt aacggataac gtttagcggt
    16621 ggcaagcagt agcggcacga caataatcac cggtgtcact tccatcagcc aggtgagttt
    16681 gtcatgggcc gaaatgccgg tataaatcag gattaacgtt agcgccagtg cgccggtgtt
    16741 aagaattaac ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg aaaatactga
    16801 ctattgacca gtaagcgtga aattacaatc gcaaaagagg tggcccaggg ggatcacctg
    16861 gcagcatgct gccaggcgct gggccgaaga ggttacttag tgcagttcgc gcactgtttg
    16921 ttgacgattt gctggaagaa gtcgttgcct ttgtcatcga ccaggataaa cgccgggaaa
    16981 tcttctactt cgattttcca gatagcttcc atacccagct ccggataagc gacgcattcc
    17041 agatgcttga tgctctgctg cgccagtacc gccgccggac cgccgatact accgaggtag
    17101 aagccgccgt gtttatgaca cgcgtcggta acctgctgac tgcggttacc tttcgccagc
    17161 atgatcatgc tgccgccgtg ggattgcagc agatccacgt aggagtccat acgacctgcg
    17221 gtggttgggc caagtgaacc tgatggataa ccggcagggg ttttcgccgg acccgcgtag
    17281 tagatcgggt gatctttgat gtactgcggc agttctttac cggcgtcaat cagctctttc
    17341 agcttggcgt gtgcaatatc tcgacccaca ataatggtgc cggtgagcga caaacgggtg
    17401 gataccgggt attgcgaaag ctgggcgagg atctctttca tcgggcggtt aaggtcaact
    17461 ttcaccgctt cgccttcacc ggcctggcgc agttcttgtg gaatgtactg gcctgggttg
    17521 tgttccagtt tttcgatcca gataccttcg cggttgattt tcgctttaat gttacggtct
    17581 gcggagcagg agacgcccat gcctaccggg caggatgcgc cgtgacgtgg cagacggata
    17641 acgcgaatgt cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc gagtttctgg
    17701 gcctcttcca gcagttcctg ttccagctgg acatcgcgga acgcctgacc atgttcgttc
    17761 ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta acttgacggt tttcaggttg
    17821 gtttccgcag acgtaccgcc aatcacaaac gcgatatggt acggcgggca agctgccgta
    17881 ccgagggtac gcattttctc gacgaggaag tttttcagtt tgccgggcgt cagcagggct
    17941 ttggtttcct ggtagagata cgttttgttg gcagagccgc cgcctttagc gacacagagg
    18001 aatttgtact catcgccatc taccgcgtac aggtcgattt gcgcaggcag gttcgtgccg
    18061 gtgttgacct ctttatacat gtccagcggc gcgttctgtg aatagcgcag gttatcttcg
    18121 atataggtgt tatagacgcc tttcgacagc gcttcttcat caccgccacc ggtccacacg
    18181 cgctgacctt ttttaccgac gatgatcgcg gtgccggtat cctggcaggt cggcagcacg
    18241 cctttggcgg cgatttcgga gtttcttaag aattgcagcg ccacgtactt gtcgttttcg
    18301 ctggcttctg gatcgtgaag aatagccgca acctgtttct ggtgtgctgg gcggagcatg
    18361 aaagaagcgt cgtgaaaggc ttgctgcgcc agcagggtca gggcttctgg ttccactttc
    18421 aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag tagatagtat
    18481 tcggtattgt ctttccccat cgggaaaggt gcctggtaga taaagggttt gtttgacata
    18541 gcttccagcc tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc
    18601 ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc gaagatccag ccgaagacgc
    18661 acgatacgct cacaccaatc aaccccggca gaataaagct gtggttgatg acgaagcgac
    18721 cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc ggataagtcg
    18781 gcaggatgta ataaccgtag caagccggtg ctgaagccac gatgtatgcc ggatcaacgc
    18841 cgatcgccag cgcgaccgga acaatcgccg ccagcgccgc agcctgagag tttacaaact
    18901 tggaaaccag cagcagaaca atggcatagg cccacggata ctctttcacc atttcaccca
    18961 gtacgccctg aatttcagac atatgcgcac cgaacatggt ttctgccatc catgcgatac
    19021 cgtacaccgc cacgatggcg atcataccgg aacggaagac ttcgtttttt gagatagacg
    19081 cgggattggt tttggtcagg ataataatca gcgccccggt cagcagcata aacatctgaa
    19141 taaccagtac catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac
    19201 caagaagggc gactacagcg attgccccga ggaagatcca cattgccagc cagttgcttt
    19261 tcggcagttt tttatccagc agcgtcgcgg tatcaccgta aacatactca cggttttccg
    19321 gtacggagat gaatttctgg aactcttcgt ctttatccag atctttaccg cggaaccagc
    19381 tgaagatacc gatcgccagg ataccgatta acgtcgatgg aatggtgatt gccagcaggt
    19441 cgaggaactc aagatggcga ccatcaaagg tgacattacc cagcatcgca accagagaca
    19501 cgactgcaac cgacaccgga ctggcgataa tccccatctg tgcaccgata gaacttgccg
    19561 ccattggacg ttccggacgg atgttgttct taatagcaac gtcgtagatg atcggcagaa
    19621 tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag cgtacaggtc acaaacggcg
    19681 cgacaattga gacatatttc gggttgcggc gcagcagctt ctcggcaatt tgcagcatga
    19741 catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca atgataacca
    19801 gcatgacatc aaccggtggt ttacctggct gaaggtggaa gacgaagacc agaatgacca
    19861 gaccgatacc gcctaataaa cccagcgcga taccaccctt tctggcacca taaaacagac
    19921 atatcagtat tatgataagt tggatagtaa ataacatgtg tgaaccctcg cgataatcct
    19981 atttaaattt ttgctgaata gatcacagtc acgttctgtt ttgtatgaac tgttttcaga
    20041 gcctgactaa atatccgttt ggtcgttacc ggctttagca aatacctcac agtgaatatt
    20101 ggctgattaa agcagcggca aatttatgca ctgtgataag cggctttttc agagagaaaa
    20161 actctctggc gatgggttta tagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt
    20221 atcttattga tatttatcgg tctatttttt gttctcaggg cagatatcat taggtttata
    20281 gattaatctg atctacccat ttgtgggtaa aaatacacat tatgcgggtg acataatagt
    20341 taattaactt ttgttagcgt tttgaaatta aaaacaccgt tcatctgaag agatattaat
    20401 ttttaacgat gatggaggga taattatatt tgatctggca caagttttac tgatgaagaa
    20461 tgtaacttgt gccaggaata ttttgcatta cagtaataat tattacttac agataaccag
    20521 cgatccgtta ttggcaatat tgtttcagca gtgagtagtg ttctgcctga atacggtaac
    20581 ggtaaaccgg acgccctgtg acgccataat ggatactggt gaacaagatg tggcagttga
    20641 ccagccagat gaggtattta cggcaggaaa cacgcgagat gttaacctcg ttggccagct
    20701 cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg
    20761 tctgcggcgt caagcctttt ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt
    20821 ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct
    20881 tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt
    20941 aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga
    21001 tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt
    21061 tctctttttg catatagata tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga
    21121 taatctcttt ggctttctcc agcgtcgaag ccgttccaca gcattgaaag cctgggattt
    21181 gtgccacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat
    21241 tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat
    21301 cccgggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag
    21361 tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc
    21421 gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcacagtgca gccagccgtg
    21481 acggtagtgc aatgttacgc taatttcgcc accgggttcc ggtcctaatg cctccagcgc
    21541 gttttctatc agatttccca gcgtggtaat cagcgtcgcg acctggtctt cactgccgct
    21601 gtctggcaac tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt
    21661 aatcttactg attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc
    21721 aatctcttct tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact
    21781 cttcagatgc aataatccga gaatcacgtg caatttattc ataaattcgt gggatcgttc
    21841 acgaagtgcg tcagcatagt tgaccagacc atcgagtcgc tgcatcagtt tacgtacttc
    21901 agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac
    21961 ggtgttgatc agtaatagcc ggtctttaat cgtaatctct tcgtcgcgcc gcggggtacc
    22021 gtcgcgtaac acttccgaga catctaccac ctgtgaccac gagtggctta gcgtcgacag
    22081 cttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag
    22141 cgtgacttca ccgcgatcgt ccacggcaac gacgccttct ttgatggact gcaacatagc
    22201 ctggcgttgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt
    22261 cagtacctta accagaatgc aggtgccaat cagcccgacc agcatgccaa ataataccga
    22321 ccagatgatg ctccagcgac tgtcattgat ctgttgggtc acgcgactta actcaaggcc
    22381 gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa
    22441 agcctgcgcc agaaaaccgc gattgatagc gacattttct tcgccattca gcgctttaag
    22501 gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgcg agtagcgaag
    22561 actttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc
    22621 gatggcctgg attccactct cctgtggttt tttctgcaag ccctgacgga tttccggcga
    22681 gtcggcgagg gtacgcgcta ctgccagtgc cttgttagct agcccgtctc gcgtcatatc
    22741 actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct
    22801 gaccattaag atcactgtgg tactcaattt catcggacgt ttgcgtaaca tgcggtaggg
    22861 caatgaatgt ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag
    22921 gcgggtaatt caaagggagt aagaatgatt ggcatatagg ggaagagact ctggcaacgg
    22981 aaactgccag tgctgtatga agattccagg gctatgctta tagtgaaaac catactgatg
    23041 agagagggaa ggtcatggat caggcgctac tggacagggg ttatcgctgt tataccggcg
    23101 aaaagatcga tgtctatttc aacactgcga tatgtcagca ttctggcaat tgcgtacgtg
    23161 gcaacggcaa gttatttaat ctcaaacgaa agccgtggat catgccggat gaagtcgacg
    23221 tcgccactgt ggttaaagtg attgatacgt gcccgagcgg cgcgctgaaa taccgtcata
    23281 aataagcgag ggtaaaatgg aaatacgcga aggccacaat aaattttaca ttaatgacga
    23341 acagggcaag caaatcgctg aaattgtctt tgtgccgacc ggagagaatt tagcgattat
    23401 cgaacatacc gatgtcgatg aaagcctgaa agggcaaggg attggtaaac agctggttgc
    23461 gaaagtcgtg gaaaaaatgc gtcgggaaaa acgaaaaatt atcccactat gcccatttgc
    23521 gaaacacgaa tttgataaaa cgcgggagta tgatgatatt cgcagttgat gggagagtac
    23581 agagtcacga tatttttcat gctctccgcg gtgtgatgca ggagagcatc tgaagggtag
    23641 ggggatgcac aaagaatggg cagagagcgc gtttttttgt cccaagtcat cccctttact
    23701 gagcaaaaaa aagaatatct cctatatgag aatcatcaat cggggttaat aagttttgcg
    23761 tccccagagc gtttaatatt gataggagtc atattatgga aggtaaaaac aagttcaata
    23821 cttatgttgt ttcttttgat tatccatcat cttattcctc agtgttctta aggttaagat
    23881 cattgatgta tgatatgagt ttctcctcta tcgtggctga tgaatatggg ataccacgac
    23941 aactgaatga aaactccttc gcaataacga catcgttagc cgcaagtgaa atcgaagatt
    24001 taatcaggct taaatgctta gacttaccgg atattgactt tgacctcaac attatgacag
    24061 ttgatgacta tttccgtcag ttttacaagt agccaggaag gataagaaga acataaaaaa
    24121 tggcac
//
LOCUS       DAZGAP010000074        24021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGAP010000074 DAZGAP010000000
VERSION     DAZGAP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.074"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..543)
                     /locus_tag="ACHLAM_002455"
     CDS             complement(<1..543)
                     /locus_tag="ACHLAM_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7853970.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSAGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGI"
     gene            complement(1501..1632)
                     /locus_tag="ACHLAM_002456"
     CDS             complement(1501..1632)
                     /locus_tag="ACHLAM_002456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853971.1"
                     /translation="MCTYVKSEAGRWSLVAGRWSLVAGRWSLVAGVVMVVFLLPRKF"
     gene            complement(1616..2551)
                     /locus_tag="ACHLAM_002457"
     CDS             complement(1616..2551)
                     /locus_tag="ACHLAM_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO7853972.1"
                     /translation="MVFQESNNREVARKYAEYITSVPLRRFVAEKVREYCGDLPLSIF
                     DGACGSGQLEQFLTVGSLYAVDIQASPLLHIPDNFPDATVKTVNENFFNAIDDVSDTD
                     TFDCVIMNPPFSLQYKAQSADIREKISAMMPYKKTSGVLDDAFYVLASMKARFSFFLC
                     FPGVGYRRGELAMRQYFGNRVVELVQISGGFADTSVSVLFVIIDNEKQSDDVVVYGVG
                     FNGETCKKSAPVTEKINTENWNRPYIAEEKVVIDIVQVTAELLVRQTRRRKLEDEHNA
                     FVWSLMSDDQRTEVSRFLSVHGLFFDAGSGCVHIR"
     gene            complement(2637..2713)
                     /locus_tag="ACHLAM_002458"
     tRNA            complement(2637..2713)
                     /locus_tag="ACHLAM_002458"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2677..2679),aa:Arg,seq:tct)
     gene            complement(2727..2803)
                     /locus_tag="ACHLAM_002459"
     tRNA            complement(2727..2803)
                     /locus_tag="ACHLAM_002459"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2767..2769),aa:Arg,seq:tcg)
     gene            complement(2813..2888)
                     /locus_tag="ACHLAM_002460"
     tRNA            complement(2813..2888)
                     /locus_tag="ACHLAM_002460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2853..2855),aa:Ile,seq:cat)
     gene            complement(2929..3987)
                     /locus_tag="ACHLAM_002461"
     CDS             complement(2929..3987)
                     /locus_tag="ACHLAM_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7853973.1"
                     /translation="MANTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGVLEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(4138..4335)
                     /locus_tag="ACHLAM_002462"
     CDS             complement(4138..4335)
                     /locus_tag="ACHLAM_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7853974.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(4551..4931)
                     /locus_tag="ACHLAM_002463"
     CDS             complement(4551..4931)
                     /locus_tag="ACHLAM_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7853975.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(4950..5939)
                     /locus_tag="ACHLAM_002464"
     CDS             complement(4950..5939)
                     /locus_tag="ACHLAM_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7853976.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(5991..6248)
                     /locus_tag="ACHLAM_002465"
     CDS             complement(5991..6248)
                     /locus_tag="ACHLAM_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853977.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(6245..7645)
                     /locus_tag="ACHLAM_002466"
     CDS             complement(6245..7645)
                     /locus_tag="ACHLAM_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO7853978.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEYEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(7642..8520)
                     /locus_tag="ACHLAM_002467"
     CDS             complement(7642..8520)
                     /locus_tag="ACHLAM_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7853979.1"
                     /translation="MMPFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            complement(8531..9457)
                     /locus_tag="ACHLAM_002468"
     CDS             complement(8531..9457)
                     /locus_tag="ACHLAM_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7853980.1"
                     /translation="MSSKILGNVWDACAAYGIKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLELLARTERD
                     KVAELKQQRRLSVLRDPSDSEPSKSEPSESVCSGVFDPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQQPA
                     APGGGRQRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(9454..9678)
                     /locus_tag="ACHLAM_002469"
     CDS             complement(9454..9678)
                     /locus_tag="ACHLAM_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853981.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGHVLRVCLVNTESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            complement(9675..9869)
                     /locus_tag="ACHLAM_002470"
     CDS             complement(9675..9869)
                     /locus_tag="ACHLAM_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853982.1"
                     /translation="MISYEIIISTTEYRNDVSVRTDVSVWHRRYKSRKTAELKAAEMC
                     ETISMKGSPVKYVTTAEVRP"
     gene            complement(9866..10498)
                     /locus_tag="ACHLAM_002471"
     CDS             complement(9866..10498)
                     /locus_tag="ACHLAM_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO7853983.1"
                     /translation="MKFRRHTLHIVAKSTIGSETPELLQATNDAPASFLLSASLHARI
                     MVGWAGEPKGSPVSCNAGSLNPVQSATSEIETSGGGTVPLLQEDAIMATVPVSPYLKI
                     EVVNGRAVIFSLYVACHFKRMHKNIVDRIEYLNCSREFLTRNFIPGTYRAYGDSVRGY
                     YITRDGLMMLQLGLSLRTMRYYESCIEAFHEAGNSLNLTAFRRNQWEARP"
     gene            complement(10527..10703)
                     /locus_tag="ACHLAM_002472"
     CDS             complement(10527..10703)
                     /locus_tag="ACHLAM_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7853984.1"
                     /translation="MQVLNRWFRDGRGRRVHVIRWESETQRVIYLRDGYPHECFSPLW
                     LFRRDFVECEAPPT"
     gene            complement(10723..11430)
                     /locus_tag="ACHLAM_002473"
     CDS             complement(10723..11430)
                     /locus_tag="ACHLAM_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7853985.1"
                     /translation="MNTTIFTDKAFMTSIEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQHEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSQIEMVAAMALEAVRQQRRLEQVEEKVTYVTETVERIKRGTIRDGYAGYRQLVAK
                     TGMSDAKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMSAFHRMMSEAEPRGA
                     RWYHPKMGLSQALGWQG"
     gene            complement(11427..11690)
                     /locus_tag="ACHLAM_002474"
     CDS             complement(11427..11690)
                     /locus_tag="ACHLAM_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7853986.1"
                     /translation="MKNEGIAKAVDIAGSQIALARRCGRAQSTICDWLNGKKKISPEF
                     VPSLVKAVDGKVQAYEFRPDLPELFPHPSLAHTEDGVCEGDKQ"
     gene            11814..12518
                     /locus_tag="ACHLAM_002475"
     CDS             11814..12518
                     /locus_tag="ACHLAM_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7853987.1"
                     /translation="MSFSDRLQLAMTIRGYSQGKLAREVGMAQSSVNKLVNGATGSRK
                     VVEIANTLNVRPEWLSYGVGPMTPEESTSCNPTPQVSYSPLENDKDVFRVEVLDIAAS
                     AGPGTFLVSDFAETVHAIEFSHDAARRLFCSRPANIIKMITVDGDSMAPTLCAGDQVF
                     VDVSVRNFETDGIYIFIFGHTFHIKRLQMQGMQLAVISDNPAYKEWFISESAEEHLFI
                     MGKVLIHQSIQYNRVG"
     gene            12695..13366
                     /locus_tag="ACHLAM_002476"
     CDS             12695..13366
                     /locus_tag="ACHLAM_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7853988.1"
                     /translation="MKHAVKVMDEIAAQIQESGSLLGMIYSYMSENGAESERVYNAIR
                     CLMRSLQKTEETASIFAEKLCEAKAMVSHGHFFGKIRTQREACGLTTTELARLLDLDE
                     EIILQWESGEFEPTISMLIPLANVLGCDPLSLLYEKDSASVIRVNAPEVHTESIGTRI
                     KSARKKLGLTEADLARMINTYSDPINDWECGICEVPADQIVPLASALNCDLIWLLTGK
                     SEAKE"
     gene            13372..13572
                     /locus_tag="ACHLAM_002477"
     CDS             13372..13572
                     /locus_tag="ACHLAM_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853989.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHDLLCAVVVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            13771..13953
                     /locus_tag="ACHLAM_002478"
     CDS             13771..13953
                     /locus_tag="ACHLAM_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853990.1"
                     /translation="MYQKTAEHEQTRVLLTIKNGKVIFIRQVHDDELVGSLSTFLFIA
                     EEAGYDVIAPADEDEG"
     gene            13959..14540
                     /locus_tag="ACHLAM_002479"
     CDS             13959..14540
                     /locus_tag="ACHLAM_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853991.1"
                     /translation="MRYAEFQAEATATGIRTGSMTIDYHDAIRRLDAGEFDHPNVKGL
                     QILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDIPNEHGTINRA
                     VVYNGKHGFMTIYPSPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITCPDKGF
                     IVSDMGREGLELLLDEIINEMNTHSMQSETSIN"
     gene            14551..14805
                     /locus_tag="ACHLAM_002480"
     CDS             14551..14805
                     /locus_tag="ACHLAM_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO7853992.1"
                     /translation="MTVIEYIQENPDCSREDISLALGRSGVSISNELSRLLWNGLIVR
                     TGEKNKMILYRINNLPFGYNNPLSVMFNQLLKQVRKSDGD"
     gene            14795..15079
                     /locus_tag="ACHLAM_002481"
     CDS             14795..15079
                     /locus_tag="ACHLAM_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO7853993.1"
                     /translation="MATDSQLTIDTALNVGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQDQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNEQD"
     gene            15066..15539
                     /locus_tag="ACHLAM_002482"
     CDS             15066..15539
                     /locus_tag="ACHLAM_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7853994.1"
                     /translation="MSKIDYQALREAAVAIETVATPQKLLAFRMKVTPQVVLALLDER
                     ERNQQYIKSRDQENDGELSELTWCSHNQHHDDTLYVRADLVNGNSPVPPDSWISCSER
                     VPAQDDWILIYSKHSEYMAGQVQGEYVELSDGTLSWLGNALYWMPLPEPPQEVRQ"
     gene            15624..15866
                     /locus_tag="ACHLAM_002483"
     CDS             15624..15866
                     /locus_tag="ACHLAM_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7853995.1"
                     /translation="MFTLIQRGQIYTDRTGYPVVITRSTEHSVFFRRMDGHSGRVRIS
                     EFNNQFEHIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            15870..16004
                     /locus_tag="ACHLAM_002484"
     CDS             15870..16004
                     /locus_tag="ACHLAM_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7853996.1"
                     /translation="MNKAFELWVHQRYGSRYDLTRDVDGFYCREIVRRMFEVWCHCCG
                     "
     gene            16023..16277
                     /gene="xisR"
                     /locus_tag="ACHLAM_002485"
     CDS             16023..16277
                     /gene="xisR"
                     /locus_tag="ACHLAM_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7853997.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            16311..17597
                     /locus_tag="ACHLAM_002486"
     CDS             16311..17597
                     /locus_tag="ACHLAM_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7853998.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            17599..18378
                     /gene="yecE"
                     /locus_tag="ACHLAM_002487"
     CDS             17599..18378
                     /gene="yecE"
                     /locus_tag="ACHLAM_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO7853999.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            18431..18826
                     /gene="yecN"
                     /locus_tag="ACHLAM_002488"
     CDS             18431..18826
                     /gene="yecN"
                     /locus_tag="ACHLAM_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO7854000.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            18867..19610
                     /gene="cmoA"
                     /locus_tag="ACHLAM_002489"
     CDS             18867..19610
                     /gene="cmoA"
                     /locus_tag="ACHLAM_002489"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO7854001.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            19607..20578
                     /gene="cmoB"
                     /locus_tag="ACHLAM_002490"
     CDS             19607..20578
                     /gene="cmoB"
                     /locus_tag="ACHLAM_002490"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO7854002.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(20743..23172)
                     /gene="torZ"
                     /locus_tag="ACHLAM_002491"
     CDS             complement(20743..23172)
                     /gene="torZ"
                     /locus_tag="ACHLAM_002491"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO7854003.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(23197..>24021)
                     /locus_tag="ACHLAM_002492"
     CDS             complement(23197..>24021)
                     /locus_tag="ACHLAM_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO7854004.1"
                     /translation="HEFVSGKIDSDDKFEAHRQEMAETVWKELKATDSATCRSCHSFD
                     AMDIASQSESAQKMHNKAQKDGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVT
                     NGAHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQR
                     LALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGVLTDPALADRKPLWQYAEKLDD
                     TYCAGCHAPIASDHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         6024 a         6244 c         5755 g         5998 t
ORIGIN      
        1 aatacccgca ttcgccggta taaacggcag cagttttttg gcgatatgca gcccctgccc
       61 cacggttccg tactgcccct ttgacaggtc cgctttcgga tggttaagac ggctcatatc
      121 ctgcacatca tgcagacagt ggtccgccgg aataatgtcg ttatacttac aggcggcacc
      181 gcccggcgtc accgtactgc gacgcgccag ctgcttaata cgcgggtccg gacggtcata
      241 tgtctccggc agcggaaggc cttcaccata tgacatgccg tttgactgcc ccgccagaac
      301 cacaacaaag taatactccg ggtcgctggt ggcgctgata actgtgcctt ctccagccga
      361 cggcttcacc accacaggtg tggtgacatc accttccgcc gcaatggcct gcatcagggt
      421 ataaggcgtg atggcgacag ggctgccaaa tggctgccac ccctccttca gtttttgagt
      481 cagtcgctcc gcaaggtctg acggcgatgc cgccctgacc acatcgtagt gtttaaatgc
      541 catgaatcct cccggtcggg ataatgttgt gagtcagatg aggggcgggc tgaagtccgg
      601 aagttacagg acaatgacag gaggaagact acagcccgca atacgaaaaa ggccgcgcag
      661 ttgcgcagag tgattactgt cgggtattat tcgccagctg aaaaattgat tacttcacgt
      721 tttgttgttt attccttgct gcccgcatct caccatgcgg gcttttttta tccataagaa
      781 agcccctccg gagaggggct ggagtagcgc tatgtaccgt tcattgcatg atgccgggtg
      841 cctcccggtg agttcagccc ggtgccaccg aacccgcgtc attctcgcta acgatcatca
      901 gagaccatac cgtacaccag ccgcccctcc gcacaggggg attcatcatg cagaaatttt
      961 ttaacaaatt ctcagtcggg aagacaacct tcaactgact gaattgtgag caacatagca
     1021 tttaacgggg aacctgtttt ctgcagtaaa aaggcccacc ggagcggacg ggcctggagg
     1081 gatagcggtc atgtgatgcc ggtttcccgg taactcagca ccggtatctg agtcaacgtt
     1141 ttctctactg ggtcatttcc gatacgccct gcctgctgac aggctttcat cacatctgaa
     1201 aatatagcac cctgactgat actgtagtac ccaaggttcc agaaactgtg atgtatccgg
     1261 cacagaaaag cccctccgga gaggggctga cgctgcgtat ctgtatcatc atgcgcatgg
     1321 tgccgggtgc ctcccggtga gttcagtatc agcacctgaa cccgcacaga aaggataagg
     1381 gatgtaacca aacacctgtc gctgatatgc ccctccgctc agggggattc accatgccag
     1441 tttcttttaa caaacgcccc gcaaatcaga caactgtcaa ccgcctgaat tgtgagacat
     1501 ttaaaatttt cggggcaaca ggaagacaac catcacgaca ccggcaacca gcgaccagcg
     1561 accagcgacc agcgaccagc gaccagcgac cagcgaccag cgaccagcct cagatttaac
     1621 gtatgtgcac acaacctgaa ccggcatcaa aaaacaaacc gtgcactgac agaaaacggg
     1681 aaacctccgt ccgctgatca tcactcatta aggaccacac aaaggcgtta tgctcatctt
     1741 caagtttccg tcttcttgtt tgccttacaa gcaactcagc cgtcacctgt acaatgtcaa
     1801 tgaccacctt ctcttcagca atgtacggcc tgttccagtt ctctgtgtta atcttctccg
     1861 tcaccggcgc tgactttttg caagtttccc cattaaaccc gacgccataa acaacaacat
     1921 catcgctttg cttttcgtta tcaataatga cgaataatac agaaaccgat gtatcagcga
     1981 acccgcctga aatctgaacc aactcaacaa cgcggttgcc aaaatactga cgcattgcca
     2041 gctcaccacg cctgtaacca accccgggaa agcacaaaaa aaatgaaaat cttgccttca
     2101 tactcgccag aacataaaac gcgtcatcca gaacaccgga ggtcttttta taaggcatca
     2161 tcgctgaaat cttctccctg atatccgcgc tctgtgcttt gtactgtagc gaaaaaggcg
     2221 gattcatgat gacacaatcg aacgtgtccg tgtcactgac atcatctatg gcgttgaaga
     2281 aattttcatt gaccgtcttc actgtggcat ccgggaagtt atccgggata tgcaacaacg
     2341 gagaggcctg tatatccaca gcatacagac ttcccaccgt gagaaactgc tccagttgcc
     2401 cggagccaca cgcaccatca aaaatactca ggggcagatc accacaatat tcccgcactt
     2461 tttcagcgac aaaccgacgc aaaggaacgg acgtgatgta ctctgcgtat tttctggcga
     2521 cttcgcgatt attggattcc tgaaaaacca tttgtgaaag gctctgtggt tgatatgcgc
     2581 cgggcgtggc acggagacaa aaaaggcccg caaaagcgag cctgggaaaa taagtgtggc
     2641 gcgttgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct ctgtccggct
     2701 gagctaacaa cgctgaatac cgataatgga ccgccatcgg ggactcgaac cccgcgcagc
     2761 cagctccgaa ggctggcgct ctgtcccgat gagctaatgg cggtatgtga tgtggtggcc
     2821 cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct
     2881 aaagggccgg gcgcaggata ataacggtac gtaactaata ctgcaatatc atccgttctg
     2941 actggctaaa tcctgaactt ccctgaccgt ctgctcaaaa cgttcagtct ccagctcaac
     3001 gccagttgca cgacgcccca gcgccatcgc ggctttgact gtcgaacccg accccatgaa
     3061 aaaatctgca accaggtccc ccggacgact gctcgcgctg attatctgct gcagcatttc
     3121 tgccggtttt tcgcacggat gtttcccggg atagtactgc accggtttat gcgtccacac
     3181 atcggtgtac ggcacctgcg ccgtcacgcc aaaataccgc cgcagatgct tatattcact
     3241 ctgcagttcc gcatactgcc ggttcagtga agtatacgtc tccagcagct ggtggtgggg
     3301 cttttccagt acaccgcgct gatgcttctc ttctgccacc cgggcaaaca gcgactgtaa
     3361 tttcagataa tcgctttcgt tcggtagctg ccactgactg gcactgaacc agtgcgacac
     3421 catgtttttc tttcctgtgg catccactat ctgttttgcc gttatcccca gggcagcacg
     3481 cgcatcacga aagtaagcaa tcagcggagc catcacatgc tgtttcagtg ccctgccctt
     3541 cgcctcatac ccggcatctt tcggacgata cggcccctga taatgttccg cgaacagaat
     3601 gcgctctgtg gcggggaaat acgcccgcag gctttccttg ttgcacccgt tccagcgtcc
     3661 ggacggcttt gcccagatga tatggttcag cacactgaag cgttcacgca tcatgatttc
     3721 aatgtcagac gccaggcgat gaccacagaa caggtaaaga cttccggcag gtttcagcac
     3781 ccgccagaac tgcgcaagac actggtccag ccatttcagg taatcctcat cccccgtcca
     3841 ctggttatcc cagccctcgg gcttcacttt aaagtatggc gggtctgtga ctatcagatc
     3901 gacagagttt tccggtaagg tctggataaa ttccaggcaa tcagcgttga ttagctcaca
     3961 actggatatt tttacagtat tagccataga tcaataagca cttctctgat aggctcattc
     4021 tgcttttgcg caaagcagtg ggcctgaggt ttgcttgtga tccggacgca tgagcagatg
     4081 gctggtgagt gcccctaaca cccaccagcc gcccatttac cacaaataaa aaagccttca
     4141 ggactgaagg cgtctgtaac aaccgaactg atagtctgcc agacccgcca taacaagctg
     4201 ggtcagtatt aactgacagc gttcgcgtga aaggtacgta ttctgtgcaa tctccccgac
     4261 tgtcgccggt tcggtgacgc ttaattcatt aaacaccact ctggcggttt ctgtcatatc
     4321 ctgctgtttc agcatgtctt tttccctttt ccggttaacg tgacacacca ataactcttg
     4381 tcgaaaaagc cagcaagctg aaagaccggt attcaccgcc accagcgcgt ttaacgtact
     4441 ggtccgattt caggcataaa aaacccgcct gacggcggat ttaagctgtg tggcaaagta
     4501 accactctta acatactgac atactttttg cggaccgcac taatcatttt ttactttttt
     4561 agcagccagt cgtccatctc cagtcttacc cccagcacag acaaacatcc gtcaataaac
     4621 ccttcggcta tctgcatctc aattcgtatt gctttttcgc ttttctttct cgtcctggct
     4681 atctgtcttt ttgatattcg caacaaataa tgagcaatga gaagcgaata ctcctcaggt
     4741 tttttctgct tcagacgtgc aagacagttt tcaatgataa gtccgtcatc atcgcagcag
     4801 gctggacgtg gtttagtggt agatggtaaa aggcctttga atccggcagc gatcggagaa
     4861 tagtccaccc cggtgttacc acttgccgcc catgcccccc agcgttcaag aaccatctga
     4921 atatcacgca tcaactttct ccacaaaaat caggacagca caccaatcgc cagtgcgcga
     4981 tcgataaaac gaaatatcag ctccagttgg gaaccatact tctcttcaaa tgccacggta
     5041 tccgcatgca gttcgtcatg gtgttttctg cacaaaggca acacaaaaag gtcatgcgct
     5101 tttgtaccca ttccaccctg accatgacca atcaggtgat gcggatcgtc ggctggctta
     5161 ccacaacatg cacacggctg cgtcttaacc cagcgcgtgt acttttcatt aacccagcga
     5221 cgacgtttgg ggcgtaacat aaaagactcc ggcgactccg gatccacttt cagcgccagc
     5281 acctttttcg ctttatcctg gataatgctg gtggcaggaa ccgaaggcac aaggtcactc
     5341 tcccgggtga cagacggcaa aacaggcttc ggtaatctca gggccttacg ggctgcactt
     5401 tccggtaagg catccgccag gtcattacga accagccacc agcacagttc aggcattgtc
     5461 acaacgtgac tgtcatcaaa accgagatca cgacggacta cggacaacac ccagcgggca
     5521 cagttatccg ttgccattga ctccagacgt tccgtgaact gatcgcgaag caggttatcg
     5581 cagtgccagc acagacggat tgcgcccggc gcgtgtcgca ttgtggtcat gttctcgctg
     5641 tgccatccgg aatgaggcca ctggcagcct ttttcacgaa gtaaccagct ctcaaggcat
     5701 tccacgccac cagcacgacg gatcaccgcc tcatggcgga acacggcccg aacggcagga
     5761 tcatccgcca gcggttgtga tgctgccgga acggcaccac tggcaaaaga tgaataacgt
     5821 tccggctcag gctccagcag gacacgcccc tgcataaaca ggggcatcag ctctgaaccg
     5881 ggtctgaaca agacgatccc catacgcggg gcaatttcag gggtcagcag tgctctcacg
     5941 gtcacctcag cgaacggtat tgcatgaacg caggagaaaa aattcagcca tcacgcagta
     6001 aactctttca ccagcgtttc aaactggctt acctggcctt ccagttccgc cacgcaatcc
     6061 accagctcat ccaccgcctt ttgtgtgcga tgttttgcct gcagcagatc acgaagcgcc
     6121 ggagtaagct gcttgcggag cgtatctttt gccacgctcg ttttttccat ctgttcagca
     6181 caacgaagca tctcctgcgc ctgccgacga agttgttccg gtgaaacagt ggttgttctg
     6241 ttgttcaaaa taaacgctcc gttttactac ccgacatgcg gttattgctg tatctgcgcg
     6301 gattgcccgg cgtcatggga gtggaaagaa cccgggcact ctcctggtcc acaggcagaa
     6361 aatgcccgtt atgaaaacgc cggtaaatgg tacccagcgt gccattacgc tgtttcgtga
     6421 tgttgatttc agctatgcct ctcgcctgtg tctccgggtt gtacacctca tccctgtaaa
     6481 gcatcagaat gatgtctgca tccgcctcta tttcccctga gtttttcagg tccgagttca
     6541 tgggacgttt attgggtctg gattccacgc cacgggagag ctggctcaga gcaatcagcg
     6601 gaaaaccgcc ggattttgcc aggcttttaa gtccctttga gatttccccc acagcaaggt
     6661 cgtgacgccc cgtgctgcgg gttttaatca ggccgaggta atcgaccacc accagcgccg
     6721 tttccgggtg tttcatccgg tggtgcctcg tggttgcaca tatctcatca atggtcaggt
     6781 ttgcctggtc caccatccag atattacgcc ccgtcattcg tcccacgccc tgtgagaaac
     6841 gcgcccagtc ttcatattca aaacgggcaa cagacttaag acgggatacc ggcattcccc
     6901 cggcagcaga caccatacgt tcaccaatct gaatgttcgc catctccatg gtgaacagaa
     6961 gcacgccatg cccctgctca gtcaccttgt cgatgatatc cagcgcaagt tcggttttcc
     7021 ccatcgaagg acgggccgca atgaatacca ggtcgcctgg ctccataccg cccgtttttg
     7081 cgtccagttc atcaataccg gtcatcagcg ccctggattt ctccagtccc tgattgcggc
     7141 attcaacacg gtcgaccact tccggaagga catcatcaat gtgaaccggc tgaatgacgc
     7201 cctttccggt cgacagtgag gccatcatgt tctgcgcatc cttcagggca tcctcggctg
     7261 cttcacaggt atgcgcatcc cgtaaattct gtaatgcttc agtcagtgtt ttttctgcat
     7321 cgcgcagtgc ggcattacgc cgcaacgctg cgacatagtg ctccagtgaa gacttcaccc
     7381 aggttttgcg tccggtgtcg gtaatcaccg gggcaagttc cggcatctca ttgcacagca
     7441 gtacggggtc aatgacgccg gatacacgtg cctgtctgca aattcccgcg taaatatccc
     7501 ggtactgtcg tgcagaaaaa acgtccgccg acaatgtggc cagaatatcc atcacttccg
     7561 gatcagcccc acgcagaaaa aacgcgccaa tgacagcgcc ttccaggtca tcgttacgcc
     7621 atgccggggt gttctggctg gtcatgcggc aacacctccg atacgagaac ggtagctggg
     7681 ccagttaaac gacaaccagt tgcgcccgcc attggtgatc ctgtcggcaa tccggggact
     7741 gatgaacgcc cacaattctt ccggtgaaag gttgctgatc aagatagttg gcaaaatacc
     7801 ctcataccgg gcattgataa tttcctgcaa aatggccatt tcagccgcac tgccaaactg
     7861 aacgccgact tcgtcgatga tcagcaaatc cagtgacgca taatgctcaa tgacgtcatc
     7921 cgctgttttt tcactgtcat tccgccagca gtttttcaca gcacgggtaa ggcgcatcac
     7981 gtcggtgatc tccacactgg ccagatagtt acggatgatg tgttttgcca ttgataccgc
     8041 cagatgattt ttcccggtac cgcaactgcc ggtcataaca agactggtac cgttctccag
     8101 catatctggc cagttctccg catagcggcg acaggccgca agatttctgg ctgcgtcagg
     8161 attaacctcc agataattat caaactcgca gtcccgaaaa cgcagagcaa ttccggcgtt
     8221 atcagtcagt tcttccgcct tgagggacga cagctccatg gtcaaatcgt tggcctcagc
     8281 tatcaagcag tcagggcagc atgaaatttt ttctctgtcc tcgccattac gatcgctcca
     8341 caccagaata tgtgtgtggt attcgccatg tttttcgcaa cacccgcgcc cttcacgcat
     8401 ccagcaggaa cgataaggcc atggtttttc gcccttctga gcaaatgcaa tctctgcccg
     8461 taactcatcc atccgcgcct gtagtcttgt ttgttgttca cgcaggttaa acggcatcat
     8521 cgctgtcacc tcagaatgtc aatttgtcac tggatttacc gaatttgtca gacatggctc
     8581 ccaggccagc caggacatcg acctgtcgct gtcgcccacc tccgggagca gctggctgtt
     8641 gccagaaatc ttcgaagtga cgatcgggtc caaagaacgt cgacgcctgc ttcacgaact
     8701 gggtgccggt atttcctgag acacgcaccc aggcagcata gcgtttcaca ccgttgagca
     8761 tggtttctgg tgtcacacct tccctgattc gggctttcca ggctttgaag gctgctgact
     8821 tggaattacc accagcacgt ttgggatatt cctgccaggc ctgttcaaat tccggtgaat
     8881 attcctgtct ggcagaacgc gctggtgcag acgcgtcagc ggatgcgcca atatcttgcg
     8941 gttcatgttt tgaatttact ggtggttcat gttttaaacc ttgtggatct ggggtcagat
     9001 tctgaagggt caaacgcgta tttttgccag aatctgaagg gtcaaacaca cctgaacata
     9061 cagattctga cggttcagat tttgaaggtt cagaatctga agggtcacgt aatactgaaa
     9121 gtctgcgctg ctgtttcagt tctgcaacct tatcccgctc tgttctggca agcagctcaa
     9181 gccgatcagc attcagatga taaagattgg acgtattacg gttacctttg cggcgtgact
     9241 gacgcgtcag ccagccatca gcctccagtt cggagattgc cgttctgact gtactttctc
     9301 ccaacccaag ctgtcggcat atggtttcaa cacctgggta gcacaccccg tcatcattcg
     9361 aataatccgc caggcgcgcc ataatcatca gttttgcacc tttgatgcca tatgctgcac
     9421 atgcatccca gacattacct aggattttgc tactcatacg gcacctccca gacgcttaaa
     9481 catttttccg gactgaaacg ccaccagcgg ataactcagg gtatgagtac gtccctgaac
     9541 ctggcagaca accttctggc tttctgtatt gactaggcaa acccgcagaa cgtgtccgtt
     9601 gctggtggtg aaccactgcc ccacacgggg gcagtggttg tatcggtgat acaggaaatt
     9661 aacgatgtgg cggatcatgg acgcacctcc gccgtagtta cgtatttaac cgggctacct
     9721 ttcattgaga tggtttcaca catctctgcc gctttcagtt ctgccgtttt tctggattta
     9781 tagcgacggt gccagacaga tacatccgtg cgaactgata catcgtttct gtattcagtt
     9841 gtggaaataa taatttcgta actaatcatg gacgcgcctc ccactgatta cggcggaaag
     9901 cggtaagatt caggctgttc cctgcctcat ggaatgcttc aatgcagctc tcgtagtacc
     9961 gcatggtgcg cagacttaac ccaagctgta gcatcatcag accatcacgg gtgatgtaat
    10021 aaccgcgcac tgaatcgcca taggcgcgat aagtgccggg aataaagttg cgggtgagaa
    10081 attcgcgtga acagttgaga tattcgattc gatcgacgat gttcttatgc atgcgcttaa
    10141 agtgacaggc gacatacagg gagaatataa cggctctgcc gttgacgacc tcgattttga
    10201 gatatgggga aactgggact gtagccatga tggcatcctc ctgtaacagt ggaacagttc
    10261 caccaccgga ggtctcaatc tcgctggtgg cggactggac agggttgaga ctaccggcgt
    10321 tacaggaaac cggcgaacct ttcggttccc ctgcccagcc caccataatt ctggcgtgca
    10381 aggacgctga caacaaaaaa gacgctggcg cgtcatttgt cgcctgtaac aattccgggg
    10441 tctcactccc gatagtggat tttgccacta tatgtaaagt gtgccgccgg aatttaattt
    10501 tgtcaacgag gttaaatata gagtcgctat gttggtggcg cttcgcactc aacaaaatca
    10561 cgacgaaaca accacagcgg gctgaagcat tcatgcggat aaccatcacg caggtaaata
    10621 acccgctgtg tttcagactc ccagcgtata acgtggacgc gacgccccct tccatcacgg
    10681 aaccagcgat tgagtacttg catgtgttac ctgtgagcat aattacccct gccagcccag
    10741 cgcctgggac aaccccattt tcgggtgata ccagcgtgca cctcgtggtt ctgcttcact
    10801 catcatgcga tgaaaagcag acatgaaggg ttctactgca acaatcgcgc gacgcgacaa
    10861 caatccatcc ggcgtcataa attcatgagt gtcggtaggg atctgatatg cgttcaccag
    10921 attgcggcat ttcgcatctg acatacccgt tttcgccacc agctgacggt agcctgcata
    10981 accatcgcgt atggtgcctc ttttgattcg ctcgacggtt tcagtaacgt aggtgacttt
    11041 ctcttccacc tgctcaagac gtctttgttg gcgaacagct tcaagcgcca ttgcagcaac
    11101 catttcgatc tggctcattg gtttgcggat ttgttcttcc agttcgcgcc agcggtctac
    11161 caggcgggcg gtgaattcag ggcaaagctg tgcgacaaca atgatgctgt cgcgtttgcc
    11221 ttgttcgcct tcaaacagat aatgctcgtg ttgaacttta aaccctaagt tattgattct
    11281 ttcggaaacc tcaatttgag gagaccggac aacaccacct ttggctaatg tttcaatagt
    11341 gcgtttcaca ttgtcatgtc gcttacccac cagctctgcg atctcaatgc tggtcatgaa
    11401 tgctttatcg gtaaaaattg ttgtattcac tgtttatctc cttcgcacac tccatcttct
    11461 gtgtgcgcta agcttgggtg tgggaaaagc tccggtagat caggacgaaa ttcatacgcc
    11521 tggactttcc catcaaccgc cttaaccaga gacggaacaa attcaggaga aatttttttc
    11581 ttcccattca gccagtcgca aattgttgac tgcgcgcgac cgcaacgcct agccagagca
    11641 atctggcttc ccgcaatatc tacagccttg gctatacctt catttttcat atcgacctcc
    11701 aacctgattt cattcactat agcgattgat taaagcgatt gcaatgtacg gaaaagcggt
    11761 ttgtgtatat atcgctaagg cgatacacta aaagggtaga aaaaggagga cttatgagct
    11821 tttcagacag gctacagtta gctatgacta tccgcggata ctcgcaggga aaactggcca
    11881 gagaggttgg catggctcaa tcgagcgtaa ataaactggt taatggtgcc acaggctcca
    11941 ggaaagttgt ggagatagcc aataccctga acgtgcgccc cgagtggcta tcgtacggtg
    12001 ttggtcctat gaccccagaa gaaagcacat cctgcaatcc gacaccacag gtatcatatt
    12061 ccccgctgga aaatgataag gatgtttttc gtgttgaggt cctggatata gctgccagcg
    12121 cagggccggg aacattcctt gtgtcagatt ttgcagaaac ggtccatgcc atagaatttt
    12181 cacatgatgc tgcaagacgc ctgttctgta gcaggccagc aaatatcata aagatgataa
    12241 cagttgatgg cgacagcatg gctcctactc tgtgtgcagg agatcaggtg tttgtcgacg
    12301 tatccgtcag aaattttgaa actgacggta tctatatttt tatttttggg cacaccttcc
    12361 atatcaaacg cctgcaaatg caagggatgc aacttgctgt tatatcagac aatccggcct
    12421 acaaggaatg gtttataagt gaatcagctg aagaacatct attcataatg gggaaagttc
    12481 tcattcatca gtcaatccag tataacaggg tcggataagt caacatcacc ggcagctgta
    12541 ttgcgcagct gctctacttt cctgtatctc aactccctac gtatcgtctc atttctctca
    12601 taatctgttc tttgctgccc aaaatacata gctatagcga ttgacatagc caaacgcttt
    12661 agctattgta aaattaaaac aacaggaggc tcatatgaag catgcagtta aggtgatgga
    12721 tgaaattgca gcacaaatac aagaaagcgg ctcgctgctt ggaatgatct attcctacat
    12781 gtcagaaaac ggagcagaaa gtgagagggt ttataatgca atcagatgcc tgatgcgttc
    12841 cctgcaaaaa acagaagaaa ctgcatcaat ttttgcagaa aaactatgtg aggcaaaagc
    12901 tatggtttcg catggtcact tttttggaaa aatccgcacc caacgtgaag cctgcggctt
    12961 gacgaccacc gaactggcca gactactcga tcttgacgaa gagattattc tgcaatggga
    13021 gagcggagag ttcgaaccga ccatcagcat gcttatcccc ctggcaaacg tccttgggtg
    13081 cgatccgctt tctttgctgt atgaaaaaga tagcgcgtct gttattcgcg taaatgcgcc
    13141 tgaagtccat acggaaagta ttggtacgcg catcaaaagc gcacgtaaaa aactgggctt
    13201 aacggaagct gatcttgccc gcatgattaa tacctatagc gaccccataa acgactggga
    13261 atgcggcatc tgtgaagttc ctgctgatca gatagtacca ctggccagtg ctcttaattg
    13321 tgacctgata tggttgttaa caggcaaatc agaagcaaag gagtagcaac aatgactggc
    13381 aatatccatg ataagtatga aggcttatgc ctggcaccgg attcctttgc aaacaatatc
    13441 catgatttat tatgcgcagt tgtcgtatta caaatgtcag acaatgacgc aataaaaaga
    13501 acaggtgatg aagttcttga gtttgctcgt tgctatgctg aagcagcagc tgagaaagaa
    13561 ctatccagtt aaataaaaca tattatcgct gaacaatata ttacggctta atcgccgggg
    13621 attatcacac ccttaatcca caggaggctt tattatgact tttgtaaaac ataaggcatc
    13681 acacaaaaca gcctgtctta ttgcacaaca cggtgaaaat tacatgcata ttgcctgctt
    13741 atttctgcgt aaagcatacg ggagataata atgtatcaga aaacagcaga acacgaacaa
    13801 accagagtat tgctgaccat caaaaacggg aaagtaatat tcattcgcca agttcatgac
    13861 gatgaacttg taggaagtct ttcaacattc ctgtttattg cagaagaggc aggatatgac
    13921 gttattgcac cagcagatga agatgagggg taaatatcat gcgatacgct gaattccagg
    13981 ctgaagcaac agccacaggt atacgaactg gcagtatgac tattgattat cacgacgcta
    14041 tacgccgtct ggatgctgga gaattcgatc atcctaatgt gaaaggtttg caaatccttc
    14101 aatgcctcgc acaagctgac gaagcaggat tactgggaaa acttccggtt gagatgaagg
    14161 ttgcccagtg gcgatggttg tatgtgacga cattcatcaa cgaagaagaa gacaagaacg
    14221 gcacaattga tatcccgaat gaacacggaa caataaatcg cgccgtagta tataacggga
    14281 agcatgggtt tatgactata tatcccagtc ccattcgttt tgccttacaa cagtatattg
    14341 aatggaattt aattcaaaaa tacggcgagg cagaaggaat gggtagagcg ctgtttcttt
    14401 atcagaaaat gctcattaca tgcccagata aaggtttcat tgtttcagac atggggcgag
    14461 aagggcttga actccttctg gatgaaatta ttaacgaaat gaatactcat agcatgcaat
    14521 cagaaacctc tattaactaa aagggactac atgaccgtta ttgaatatat ccaggaaaat
    14581 ccagattgca gtagagaaga tatatccctc gcacttggaa gaagcggagt ttctatcagt
    14641 aatgaattat cacggttact gtggaatggg ttaattgtac gaactggtga aaaaaacaaa
    14701 atgattctgt atcgcataaa caatctgccg tttggataca acaatcccct gagcgttatg
    14761 ttcaaccagt tacttaaaca ggtaagaaag tctgatggcg actgactcac aactaaccat
    14821 agatacggct cttaatgtcg gtctggcgct ccttggttat ttctacatca tgttctgcag
    14881 cggacgatgg ctgtcactgt tgttcatgaa aaaatggaat aaacgccgta agcaggatca
    14941 acgccagaag gcaatggatg catttttcga agccttcggg attgacggca tggaaccagg
    15001 ggatccagct cgcgcaatta gcagaggggg cgtagtaatc cttgtatacc ggagtgaaga
    15061 gaaaaatgag caagattgac tatcaggcac tgcgtgaggc ggcagtagca attgaaacag
    15121 tagcaacgcc tcaaaaattg ctggcatttc gtatgaaagt cacacctcag gttgtgctgg
    15181 cactgctgga tgaacgggaa agaaaccagc aatacatcaa aagccgcgac caggagaacg
    15241 atggcgagtt aagcgaatta acgtggtgca gccataatca gcaccatgat gacacgctat
    15301 atgttcgagc tgaccttgtg aatggcaact ctccggtacc tccggatagt tggataagct
    15361 gtagtgagcg ggtgcccgct caagatgatt ggattttaat ttattcaaag cacagcgagt
    15421 atatggcagg acaggtacaa ggggaatacg tggagttgag cgacggcact ttatcgtggt
    15481 tagggaacgc cttgtactgg atgccgctac cagaaccgcc gcaggaggtg cgccaatgag
    15541 ctggcctgat gcatttgcaa tagtaggtat tgcaatgtca atcgcactga ttgttttttc
    15601 aatttgccgc tggggataac cacatgttca cacttattca acgcgggcaa atatacacag
    15661 acaggaccgg ataccctgta gtgattactc gcagcactga gcactcagtg ttctttcgac
    15721 gcatggacgg acactccggt cgagtacgca tcagtgaatt caacaaccag tttgaacata
    15781 ttgatcacca ggaataccga caaatactgg cagaaacaga gcaggaagct cacctgaaaa
    15841 aattacgcgc catgaaaagg aagtgaagag tgaataaagc atttgaacta tgggtccacc
    15901 agcgttatgg cagtcgctat gacctgacgc gagatgttga cggtttctat tgtcgcgaaa
    15961 ttgtgagacg aatgtttgaa gtgtggtgcc actgctgcgg ataaacgttt taggagcttg
    16021 gcatgcagac aatcatctat cagataaccc ccagcaaatg gtgtacggag agagtcctca
    16081 ttgcatcaac agggctaaag cctggcacca tcgagcgggc cagaagaaag tcatggatgc
    16141 agggaaaaga ataccgccat tacgctgtag aaggtgatcc agggcattac agtgaatgcc
    16201 tgtacaacat cgaagaaatt atgcgatgga tcgaaaacca gaaacaacca ggtgtcaaaa
    16261 atgcaagttc cggttaacct gttaatgctc ctggacgtct gggaggttca atgagtaaca
    16321 catcataccc gacaggcgtt gaaaaccatg gcggatcact ccgcatatgg tttcactata
    16381 acggcaaacg tgtcagagaa aacctcggtg ttcctgacac agccaaaaac cggaagattg
    16441 caggcgaact caggacttca gtttgttttg caatcagaat ggggaatttc gactacgctg
    16501 cgcagtttcc tgagtcccca aatctgaaac actttggtct gggaaaaaag gagataacca
    16561 ttaaggcgct ttcagaaaaa tggctggatc tgaaaaaaat cgaaatagga ggtaacgcat
    16621 tcaatcggta tcaatctgtg gtgagaaaca tgttgcctcg tataggggaa agccgactgg
    16681 cttcatctgt aacaaaagaa gatttactct tcatcaggaa agatttattg accgggtatc
    16741 acaatctctc aaacggcaaa acaacacctg ttaaggggag atctgtagtt acagttaatt
    16801 actacatgac gacaattgca ggtatgtttc aatttgcagc tgacaacggc tacacggaat
    16861 caaacccgtt taacggcctg acaccattaa aaaggtccag aaccgagcca gatccgctca
    16921 cgcatgacga attcattcgt tttattgatg cttgccacca tcaacaaacg aaaaacctgt
    16981 ggtctttagc agtatacaca ggcatccgtc acggtgagct aatatctctc gcctgggagg
    17041 atattgattt aaaagctaaa acaatgacta tccgtcgtaa ttatacaaaa ctcggggagt
    17101 ttactctacc aaaaaccgaa gcgggaacag atcgtgttat tcatcttgtt caaccagcca
    17161 ttgaaaccct gaaaagtcag gctgaaatga caagatttgg cactcaatac cagattgacg
    17221 tcaaactgcg ggagttcgga cgcaccgtaa gccatgaatg cacgtttgtc tttaatccgc
    17281 aactggtgaa aaagtgccag caagccggtt accactacaa agcggactcc atcagggatt
    17341 catgggcatc agccctgaga cgggccggac tacggcacag gaaagcctat cagtcaaggc
    17401 atacttatgc ctgctgggca ctggccgcag gagcgaatcc aagctttatc gcaaaccaga
    17461 tggggcacgc aaacgctcag atggtattca atgtttacgg agcatggatg aaagataaca
    17521 atatcgggca gatagaactg ctcaataagg agttagctga aagtgtccca tacatgcccc
    17581 acagagcaag acgctgaaat aatttctttc agtatcattg agttaaccca tggttcccgc
    17641 catgttaact gcgtggaggg taacaccacg ctttatgccc tgccgaaacc cgaggttgtc
    17701 ctgcgctggc gtgagcagac cacagatgac ttccgcttct gttttaagtt tccggcgacc
    17761 atttcgcatc aggcagcatt acggcattgc gatgatttag tgactgaatt tttgacccgc
    17821 atgtcaccgt tggctccgcg cattggacaa tactggctgc aactgcctgc cacattcggc
    17881 ccacgagagc tgcctgcgct ttggcatttt ctcgattctc ttcccggtga atttaattat
    17941 ggggtggaag tccgccatcc acagtttttc gccaaagggg aagaggaaca aacgcttaat
    18001 cgcggtttac atcagcgcgg cgttaatcgg gtgattttag acagccgccc ggttcatgca
    18061 gcacgtccac acagtgaagc tattcgcgac gctcaacgaa aaaaacctaa agttccggta
    18121 catgctgtac tgacggcgaa aaatccactg atccgtttta tcggtagtga tgatatgacg
    18181 caaaaccggg agttatttca ggtctggtta caaaaattag cacagtggca tcagaccact
    18241 acgccttatc tttttttaca tacgccagat attgcccagg ccccggaact ggtacatacc
    18301 ctgtgggaag acttacgtaa aacgcttcca gagatcggag cagttccggc tattccacag
    18361 caatcttctc ttttctgaat ttgccaccta tcatagacag gtgccatcgg ccattttaaa
    18421 gggagtttgt atggtaagcg cgctgtatgc cgttttaagt gcgttgttat taatgaagtt
    18481 ctcttttgat gtcgttcgcc tgcgaatgca gtaccgcgtt gcctatggcg acggcggttt
    18541 tagcgaactg caaagcgcta ttcgcattca tggtaacgcg gtggaatata ttcctatcgc
    18601 gattgtgttg atgctgttta tggaaatgaa tggcgcagaa acctggatgg tgcatatttg
    18661 cggcatcgtt ttgcttgctg gtcgtctgat gcattattac ggttttcatc accgtctgtt
    18721 ccgctggcga cgttctggca tgagcgcgac ctggtgtgcg ctgttgctga tggtgctggc
    18781 gaatctttgg tatatgccct gggagttggt tttctccctg cgttagcgca caatacgcca
    18841 ctttcttttt cccggatttt tacgttatgt ctcaccgcga cacgctattt tctgccccta
    18901 tcgccagact gggcgactgg acctttgatg aacgggtagc tgaagtcttc ccggatatga
    18961 tccagcgttc cgttcccggc tattccaata ttatttccat gattggtatg ttagccgagc
    19021 gcttcgttca acctggtacg caggtttacg atctgggttg ttctctgggc gcggcgacgc
    19081 tctcggtgcg tcgcaacatt catcatgata attgcaaaat tattgccatc gataactccc
    19141 ccgcgatgat tgaacgctgc cgtcgtcata ttgacgccta taaagcccct acgccagtag
    19201 acgtcattga aggtgatatt cgcgatatcg ccattgaaaa cgcatcgatg gtggtgctga
    19261 attttaccct gcaattcctg gaaccttccg agcgccaggc gttactggat aaaatttatc
    19321 aagggctgaa cccgggcggc gcgctggtgc tttcggaaaa attcagtttc gaagatgcca
    19381 aagttggtga actgttgttc aacatgcacc acgactttaa acgtgccaac ggttacagcg
    19441 aactggagat cagccagaag cgcagcatgc tggaaaacgt gatgctgacc gattccgtgg
    19501 aaacccataa agcacgcctg cataaagctg gttttgaaca tagtgagctg tggttccagt
    19561 gctttaactt tggttcactg gtggcattaa aagcagagga cgctgcatga tcgactttgg
    19621 taacttttat tctctgattg ccaaaaatca tctttcacac tggctggaaa cgctgcccgc
    19681 gcagattgct aactggcagc gcgagcagca gcacgggctg tttaagcagt ggtccaacgc
    19741 ggtggaattt ctgcctgaaa ttaaaccgta tcgtctggat ttattgcata gcgtaaccgc
    19801 cgaaagcgaa gagccactga gcgccgggca aattaaacgc attgaaacgc tgatgcgcaa
    19861 cctgatgccg tggcgcaaag ggccgttctc actgtatggc gtcaacatcg ataccgaatg
    19921 gcgttccgac tggaaatggg atcgcgttct gccccatctt tctgatttaa ccgggcgcac
    19981 cattcttgat gtcggctgtg gcagcggtta tcacatgtgg cgcatgattg gcgcaggcgc
    20041 gcacctcgcg gtgggaatcg accctacgca actgtttctg tgccagtttg aagcggtgcg
    20101 taaactactg ggtaacgatc agcgcgcaca tttgttaccg ttaggtattg aacagcttcc
    20161 ggcattgaaa gcctttgata ccgtcttttc gatgggcgtg ctttatcatc gtcgttcacc
    20221 gctggagcat ctctggcagt taaaagacca actggtgaat gaaggtgaac tggtgctgga
    20281 aacgctggtt attgatggtg atgagaacac ggtgctggtg ccgggcgatc gttacgcgca
    20341 aatgcgtaat gtctatttca tcccttccgc gctggcgctg aaaaactggc tgaagaagtg
    20401 tggttttgta gatatccgca ttgcggatgt gagcgttacc accacagaag agcagcgacg
    20461 caccgaatgg atggtcaccg agtctctggc cgattttctc gacccgcatg atccgggtaa
    20521 aacggtggaa ggttatcctg cgcctaaacg cgcggtgctg attgcgcgca agccgtaaag
    20581 gtctggtatt actgccggat gcggcgtgaa cgccttatcc ggcctacaaa accttgctaa
    20641 ttcaatatat tgcagggact atgtaggcct tataagcata gcgcatcagg cagttttacg
    20701 tttgcataac ctcagcgccc gtttccgggc gcttttcacg tcttatgcct gaacagctgg
    20761 ctgatcaaac gccgttaact tcggtgcatt gcccgtatat ttttcgatat acaccagcgc
    20821 agagttaccg gcacaggcat ttgccagctg agagctgggg atatccgccg ttaacacgtt
    20881 cgcactgccg tttttacaca agccattttc cagatctggc catgcacctt catgcacgca
    20941 taccacgcct tttttgatcc cgtcagtcac caccgcgcct gtcagaatct gaccacgttt
    21001 gttccagaca cgcaccagat cgccattcgc aataccaaag cgagcagcat cttcggtgtg
    21061 aatagtgatc ggttcacgat cggcaaccgc atatttttta cgcagttccg catagttaag
    21121 ctgactgtgt aaacggtgtg ccggatgtgc ggtcagaagc tgcaactgct tctcgtcggc
    21181 ggaacctttc cactcatcag gcgcaagcca ggttgggtgt gccgggcaat ccttatagcc
    21241 aaatttttcc agcgttttgg agtaaatctc aattttgccg cttggcgtac ccagcgcatt
    21301 tttcaccgga tcggcgcgga aatcaccata acgaacgtat tgttcgttct tctcactgcg
    21361 gcgcatttcg atcagtttat tttgctgcca gaaagcatta aacatcggca tagtcacgcg
    21421 ttgcgcacgg gcacctttct gagcggcatc atagaaaaat ttcagccacg ccatttcatc
    21481 tttaccttcg gtatagatct cttttccgcc aggtttgagt aattcagcaa gatcggcaaa
    21541 cacgtcaaaa tcgttgcgcg cttcaaattg cggagcgaca gcctgcttca tcggcacaat
    21601 atgctggttg ctgtaatcac cggtcatcgt caggtcattg cgctcaaacg atgtggtgat
    21661 cggtaatacg atatctgcat gtttagcagc tgcggtccag tagcattcag aaacgacgat
    21721 catctccggt ttctgccatg ctttaatcag acgattagta tcctggtgat gggtaaagtt
    21781 accgccaccc gcccaccaga tcattttgat attcggatac gtttgttctt taccgttatg
    21841 ctgatatttg ccgccaggat tttccagcgc atcgacgata cgggcaacag gaatcgccgt
    21901 cattccgcca tcatcagcgg cctcgctggc ctgtccggca atcgccgcag acatttccgg
    21961 cagcacgccg ccaacacgcg tcgggttgcc accgttggag taatgataag agaatccaaa
    22021 accgccgccc ggtgtaccaa tttgccctaa catcgccgcc agcgtcacca gcatccagtg
    22081 tttctgttca ccgtattgct ggcgttggat cccccagcct gccatcagca tcgtgcgatt
    22141 cgccgccatc agttctgcaa gtttgacaat ctgcgcttcc ggtacgccag taatttccgc
    22201 cgcccaggcc gcgctcttcg gcgtattatc gctcttacct gtcagatact cttcaaactg
    22261 cggatacccg gtagtgtatt tttcaaggaa cactttatcg tgtttgcctt gtgtcatcag
    22321 ggtatgcgca atccctaaca tcagtgccac gtcggtgccc atattcggtg cgatccaggt
    22381 ggcattatcg ccaaagaatt cgatggtttc ggagcagata ggatcaatgg caatcactgg
    22441 tttgccagat tttttcagct gatggaagta ttccagccct tgctcatcgg tactgctcca
    22501 ggcaattttg agcgtgttca gcgggttcat cccccacagc accacaacct ggctgttttc
    22561 cagaatcagc ggccaggagg tctgctgttc atacacctct acagaaccga ccacatgcgg
    22621 catgatcacc tgtgccgctc cggttgaata atcgccgcta tgcccggaat aaccgcccgc
    22681 caggttcata taacgttgca gtaaggtttg cgctttatgc aacacgccag aagaacgcca
    22741 gccgtaagat ccggcaaaaa tggccgatgg tccgttagct ttacgaatac gatcgtgttg
    22801 ctcatgaatc agttttaatg cttgttccca gctcacctgt acataggtat cttcgccacg
    22861 acctttcgcc ggttgcagtg gattatcgag atagcttttt ctcaccatcg gatgctgaat
    22921 acgcgccgtg gtgtgtacct gatccgccgc cgtagactgt aaggagttcg gtatggtttt
    22981 cgccagcgcg cctgtcgaag aaacaatctt gccgtccttc acttctacgt tcatcgctcc
    23041 ccaacggccc gcggtgagaa ttttgccgcc cttctcttct gcccatgcag gtaacggtgc
    23101 agccgatgtc accaccagcg cccctgccgc aataccgcta tgtttaataa attcacgtct
    23161 tgttaatgtc ataacttcct ccctgatcaa cgaggatcac tgtttctcgg taatatcttt
    23221 ggcgttgtac tggaagtacc gcgtcaaaat gtccagttcg ttttcgctca tgctggttcg
    23281 cgcacccatt cctttggcaa tggacggcca tgcattgacg gtgtaatggt cggaggcaat
    23341 aggggcatgg caaccagcgc aataggtatc gtcaagtttt tcagcgtatt gccatagagg
    23401 tttacggtcc gctaatgcgg gatcggtaag cacaccctgt aaagacgcct gacgccattg
    23461 attgccgtat tcgtcagcct gccattcccc gtttaccgtc agcgccttga taccttcttc
    23521 acttaatgtg gctagcgcca gccgttgacc tgccgccagg tagagcgtgt tttcactgcc
    23581 ctgcatttga taaccctgca acagaacgat cggctgtttg ccactggcat caacgacggt
    23641 gagatcggta ccaggattca cggtagccag ttcgcctatg cgagaagttt tgaaaggata
    23701 aatatgtgcg ccattagtaa ctgaagtagc ggcctgactt tccagctcat gcgccgcgtt
    23761 gtcatccatt tttatttctg gcggaaaatg ggcaatgcct ttatggcaat cgatacaggt
    23821 ttcgccgtcc ttttgcgctt tattatgcat tttctgcgca gattcacttt gcgaggcaat
    23881 atccatggca tcaaaagaat ggcaactacg gcaggttgca gagtcagtgg cttttaattc
    23941 tttccatact gtttcggcca tttcctggcg atgagcttcg aacttatcat cactgtctat
    24001 tttgccgcta acaaattcat g
//
LOCUS       DAZGAP010000075        23992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGAP010000075 DAZGAP010000000
VERSION     DAZGAP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.075"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..151
                     /locus_tag="ACHLAM_002493"
                     /pseudo
     CDS             71..151
                     /locus_tag="ACHLAM_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(196..2241)
                     /gene="dcp"
                     /locus_tag="ACHLAM_002494"
     CDS             complement(196..2241)
                     /gene="dcp"
                     /locus_tag="ACHLAM_002494"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO7854005.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KSAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            2378..3124
                     /gene="ydfG"
                     /locus_tag="ACHLAM_002495"
     CDS             2378..3124
                     /gene="ydfG"
                     /locus_tag="ACHLAM_002495"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO7854006.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3213..3899
                     /gene="rspR"
                     /locus_tag="ACHLAM_002496"
                     /gene_synonym="ydfH"
     CDS             3213..3899
                     /gene="rspR"
                     /locus_tag="ACHLAM_002496"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO7854007.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4076..4279
                     /gene="ydfZ"
                     /locus_tag="ACHLAM_002497"
     CDS             4076..4279
                     /gene="ydfZ"
                     /locus_tag="ACHLAM_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO7854008.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4315..5775)
                     /gene="ydfI"
                     /locus_tag="ACHLAM_002498"
     CDS             complement(4315..5775)
                     /gene="ydfI"
                     /locus_tag="ACHLAM_002498"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO7854009.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5865..7232)
                     /gene="ydfJ"
                     /locus_tag="ACHLAM_002499"
     CDS             complement(5865..7232)
                     /gene="ydfJ"
                     /locus_tag="ACHLAM_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO7854010.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVTAAQ
                     PSRSL"
     gene            complement(7290..8309)
                     /gene="rspB"
                     /locus_tag="ACHLAM_002500"
     CDS             complement(7290..8309)
                     /gene="rspB"
                     /locus_tag="ACHLAM_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO7854011.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(8321..9535)
                     /gene="rspA"
                     /locus_tag="ACHLAM_002501"
     CDS             complement(8321..9535)
                     /gene="rspA"
                     /locus_tag="ACHLAM_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO7854012.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(9741..10067)
                     /gene="ynfA"
                     /locus_tag="ACHLAM_002502"
     CDS             complement(9741..10067)
                     /gene="ynfA"
                     /locus_tag="ACHLAM_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO7854013.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            10202..10543
                     /gene="ynfB"
                     /locus_tag="ACHLAM_002503"
     CDS             10202..10543
                     /gene="ynfB"
                     /locus_tag="ACHLAM_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO7854014.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            10578..11138
                     /gene="speG"
                     /locus_tag="ACHLAM_002504"
     CDS             10578..11138
                     /gene="speG"
                     /locus_tag="ACHLAM_002504"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO7854015.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(11141..11851)
                     /gene="ynfC"
                     /locus_tag="ACHLAM_002505"
     CDS             complement(11141..11851)
                     /gene="ynfC"
                     /locus_tag="ACHLAM_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO7854016.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            11959..12264
                     /gene="ynfD"
                     /locus_tag="ACHLAM_002506"
     CDS             11959..12264
                     /gene="ynfD"
                     /locus_tag="ACHLAM_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO7854017.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            12463..14889
                     /gene="ynfE"
                     /locus_tag="ACHLAM_002507"
     CDS             12463..14889
                     /gene="ynfE"
                     /locus_tag="ACHLAM_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO7854018.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLVNENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            14950..17373
                     /gene="ynfF"
                     /locus_tag="ACHLAM_002508"
     CDS             14950..17373
                     /gene="ynfF"
                     /locus_tag="ACHLAM_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO7854019.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            17384..18001
                     /gene="ynfG"
                     /locus_tag="ACHLAM_002509"
     CDS             17384..18001
                     /gene="ynfG"
                     /locus_tag="ACHLAM_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO7854020.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            18003..18857
                     /gene="ynfH"
                     /locus_tag="ACHLAM_002510"
     CDS             18003..18857
                     /gene="ynfH"
                     /locus_tag="ACHLAM_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO7854021.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            18900..19514
                     /gene="dmsD"
                     /locus_tag="ACHLAM_002511"
     CDS             18900..19514
                     /gene="dmsD"
                     /locus_tag="ACHLAM_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO7854022.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            19673..20965
                     /gene="clcB"
                     /locus_tag="ACHLAM_002512"
     CDS             19673..20965
                     /gene="clcB"
                     /locus_tag="ACHLAM_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO7854023.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(20918..21613)
                     /gene="bioD"
                     /locus_tag="ACHLAM_002513"
     CDS             complement(20918..21613)
                     /gene="bioD"
                     /locus_tag="ACHLAM_002513"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7854024.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(21738..22958)
                     /gene="mlc"
                     /locus_tag="ACHLAM_002514"
                     /gene_synonym="dgsA"
     CDS             complement(21738..22958)
                     /gene="mlc"
                     /locus_tag="ACHLAM_002514"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO7854025.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHIGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(23093..23986)
                     /gene="ynfL"
                     /locus_tag="ACHLAM_002515"
     CDS             complement(23093..23986)
                     /gene="ynfL"
                     /locus_tag="ACHLAM_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7854026.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMMDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
BASE COUNT         6157 a         6169 c         5755 g         5911 t
ORIGIN      
        1 aaacatattt cagaacaatc agatttataa atttgaaaat tattataacg gaagggtata
       61 caaaaaaagt aattttgctg gcgactgcga aacggcattc gcattagcgg tgaagtttgc
      121 cctcacccag cggctgcaat tactgctata acgaggctgg tctggcgata aatccaggcc
      181 agccattggt ggtgtttata cgttcaagcc acgatgttgc agcatcggca taatctgagg
      241 tgccttaccg cgccattgtc gatacaggcg ttccagatct tcgctgttac ctctggaaag
      301 aatcgcctcg cgaaaacgcc gcccattttc acgcgttaat ccgccctgct caacaaacca
      361 ctggtaacca tcatcggcca acatttgcgt ccacagataa gcgtaataac ctgcggcata
      421 tccgccacca aaaatatggg cgaaataact gctgcgatag cgtggcggta tagcaggaag
      481 atccatattt tccgccacca gcgcccgcaa ttcaaaatca tcgacatcct gcattgcttc
      541 gttttcttcc aggcaatgcc agcgcatatc gagaagtgcg gcgctaagca gttcgctcat
      601 ctcataccct ttattgaaca ggctggcatt acgcattttc tgttgcagtt catcaggcat
      661 tgctgccccg ctctgataat gccgggcgta gcgagcgaat acctgcggat gcgttgccca
      721 gtgttcgttg atttgcgacg gaaattcgac aaaatcacgc ggcgtgttgg tgccggaaag
      781 cgtggcataa cgctggcggg caaaaaggcc gtgcagcgta tgaccaaatt catggaataa
      841 ggttatgaca tcatcccaga gtaacaacgc aggctcccca gcggcggatt tctgataatt
      901 gcagacgtta taaattaccg gatgcgtttc attcagcgtt gattgctcaa caaaattgcc
      961 catccatgca ccgccgcttt ttgaatcacg ggcgaagaaa tcaccgtaaa ataacgccag
     1021 ccccacgcca ttatgatcaa aaatttccca cacacgaacg tcaggatggt agacaggaat
     1081 atcaaaacgt tcgacaaact taataccgaa gagctgattc gcggtccaga atacaccttc
     1141 attcaacacc gtgtttaatt caaaatatgg cttgagctgc gattcatcga gatcgaattt
     1201 ctcacgtctt acctgctcgg cataaaatgc ccagtcccac ggctgcgcgc taaacccgcc
     1261 ctgctgctta tcaataaccg cctgtatgga gactaattca tcgctcgcac gttgacgcgc
     1321 cgctggaaca atttcccgca taaagttgag tgctgcttct ggcgtttttg ccatctgatc
     1381 ggcgattttc catgcggcat aatgagggaa accaagcagt ttcgcctgct gcgcgcggat
     1441 ctccaccaga cgttgaatga tagcgcgggt atcattggca tcattttttt ccgctcgcgt
     1501 ccagcccgca gtgaacagtt tttcacgcgt cgcgcgatcg cgcagttcag caagcgctgg
     1561 ttgctgggtg gtattcagca acggaatcag ccatttgtta tccagacctt tctcgcgagc
     1621 cgcctctgcc gccagcgcaa tctcttgctc actcattcct gccagctgcg cgaaatcgtt
     1681 cacaaccaga ccgccggatt tatttgctgc cagtaaccgc tggttaaact ggctggtcag
     1741 ggtcgcagct tctgtattca gtacttttaa ttttgcttta tcagcttgtg caagtttggc
     1801 tccggcaagg acaaaacgtt gatgaatgac ctccaccagg cggatggatt cactatcaag
     1861 tcccagggat tcacggcgtt gccagacagc atctacccgc gcgaataatt caccgttcag
     1921 atagatatca ttagccagtt ccgccagttc agcggaaaac tgttcatcaa gacgctgtaa
     1981 ttcatcatta gtatgcgccg cagtcatcgc aaaaaagacg ctggtaacgc gggtaagtaa
     2041 ttctccgctt tgttccagtg ccagaatagt attgttgaaa tcaggcgctt gcgggttaag
     2101 cgcgatggca gcaatttctg cccgcttttg ctgcattccc tcatcgaatg ccgggcgata
     2161 gtgatgattg gcaatttgat caaaatgggg agccagatac ggcagtgtgc tttgcacaag
     2221 gaaaggattc attgttgtca ttttcttctc ctgaacgcga ggtgttccat agcgtaggct
     2281 tactgataac ggagtgcaat cttgcaaacc agtattaccc gctcttaagc atcacgtgct
     2341 atgttattga cacacaaaag cgttgaggaa cagtgagatg atcgttttag taactggagc
     2401 aacggcaggt tttggtgaat gcattactcg tcgttttatt caacaagggc ataaagttat
     2461 cgccactggc cgtcgccagg agcggttgca ggagttaaaa gacgaactgg gagataatct
     2521 gtatatcgcc caactggacg ttcgcaaccg cgccgctatt gaagagatgc tggcatcgct
     2581 tcctgccgag tggtgcaata ttgatatcct ggtaaataat gccggcctgg cgttgggcat
     2641 ggagcctgcg cataaagcca gcgttgaaga ctgggaaacg atgattgata ccaacaacaa
     2701 aggcctggta tatatgacgc gcgccgtctt accgggtatg gttgaacgta atcatggtca
     2761 tattattaac attggctcaa cggcaggcag ctggccgtat gccggtggta acgtttacgg
     2821 tgcgacgaaa gcgtttgttc gtcagtttag cctgaatctg cgtacggacc tgcatggtac
     2881 ggcggtgcgc gtcaccgaca tcgaaccggg tctggtgggt ggcaccgagt tttccaatgt
     2941 ccgctttaaa ggcgatgaca gtaaagcgga aaaaacctat caaaataccg ttgcattgac
     3001 gccggaagat gtcagcgaag ccgtctggtg ggtgtcaacg ctgcctgctc acgtcaatat
     3061 caataccctg gaaatgatgc cggttaccca aagctatgcc ggactgaatg tccaccgtca
     3121 gtaattttta tacccggcgt aactgccggg ttattgcttg tcacaaaaaa gtggtagact
     3181 catgcagtta actcactcac aagcaagaac gaatgaccgt cgaaacgcaa cttaatccca
     3241 cacagcctgt caatcagcag atttatcgta ttcttcgtcg cgacattgtc cattgcctga
     3301 ttgcaccagg cacaccgttg tcggaaaaag aagtttctgt tcgtttcaat gtgtcacgcc
     3361 agccggttcg tgaagccttt attaaactgg cggaaaacgg cctgattcaa attcgtccgc
     3421 agcgtggcag ctacgtcaac aaaatttcca tggcccaggt gcgcaacggc agttttatcc
     3481 gtcaggccat tgagtgcgcg gtggcgcgtc gggcggcgag catgattacc gaaagccagt
     3541 gctaccaact ggaacaaaat cttcaccagc aacgcattgc catagagcgc aagcaactgg
     3601 atgatttttt tgaacttgat gacaacttcc atcaactcct gacgcagatt gccgactgtc
     3661 aactggcgtg ggataccatt gagaacctga aagcgaccgt tgatcgcgtg cgctatatga
     3721 gtttcgacca cgtttctcca ccagaaatgc tgttacgcca gcatcttgag attttctctg
     3781 ccctgcaaaa acgtgatggc gatgcggtag aacgtgcaat gacgcaccat ttgcaggaaa
     3841 tcagcgaatc cgtgcgccag atccgtcagg aaaacagcga ctggtttagc gaagagtaat
     3901 tcatttcctc tcatcccatc cggggtgaga gtttttccct ccgtcatatg gctcatgcat
     3961 gcatcaaaaa agatgtgagc ttgatcaaaa acaaaaaata tttcactcga caggagtatt
     4021 tatagtgcgc ccgttacgtg ggcttcgact gtaaatcaga aaggagaaaa cacctatgac
     4081 gacctacgat cgtaaccgta acgcaatcac cactggcagc cgtgttatgg ttagcggcac
     4141 cggtcacact ggcaagatcc tgtcgattga tactgaaggt ctgaccgctg agcaaatccg
     4201 ccgcggaaaa accgtagttg ttgaaggttg tgaagagaaa ctggcacctc tggacctgat
     4261 tcgtctcggc atgaactaag cgtgtgaatg ccgccgatgg cggcattgct ttttttactt
     4321 cacggaatat ttcgccacgg tcgctttcgc accatgcgct aataaagaca agtacgcttc
     4381 cgtcactttt gcagtaaaca agctattgtc tggcaaatca tcaccaaaga tcgccttaat
     4441 cgccagcaat gactggacgc gcgctttccc ttcggcacta ctttgtacag ccttctgaat
     4501 aacaggtaac agtgggtcac tgatttctat cggatttccc tgttcatcaa caccaccgac
     4561 ataacgcatc caacctgcga cgcccagcgc cagcagatcg aacttgctgt catgcgccag
     4621 atgccagcga acagaatcca acatccgctg tggcaatttc tggctaccat ccatcgcaat
     4681 ctgccaggtt cgatgacgta gcgccgggtt gctatagcgt tcaattaatc ggttagcgta
     4741 atcttgcaaa tcaacgccct gcactttcaa cgtcggcgct tgttcctgca acatcaaggc
     4801 atacgctgca tgacgataat gttcatcttc catgcagtca ttaatgtgct gatatccggc
     4861 aagatacccc agatacgcca ggaatgaatg actgccgttg agcatgcgca acttcatctc
     4921 ttcataaggc agcacatcac taaccagttc ggctcccgct ttttcccatt ccggacgtcc
     4981 ggcaacaaag ttatcttcta ttacccactg gcggaaaggt tcacaggcaa cgcccgcagg
     5041 atcgcgcaca ccggtaagtt gttcgatttt cgccagcgta tcctccgtca ctgcgggcac
     5101 aatacggtcc accattgttg atgggaaagt cacgttttct tcgatccatt gtgccagttt
     5161 tacatcaaca gcttgcgcgt aggaagtgac aacgtcacgc ataacatgac cgttttctgg
     5221 catgttgtca catgacatga cggtaaatgc gggaagtcct gccgctttac ggcgagccag
     5281 cgcctcgaca atcacccctg ttgcagtttt cggctggtgg ggattttgta cgtcggcaac
     5341 gaccatcggg tgatcgagca ttaactgtcc ggtcgccgga gagtggaaat accctttttc
     5401 ggtgattgtc agagagacaa tcgcgatttg cggttcacac atcacagcca acacggtttc
     5461 taagccatca atctgtacgt gcaaggcttt tttaacgacg ccaacgacgc gagccgtcca
     5521 cgcatcggcc gacatttccg caacggtata aagattatct tgctgttgta aatcggcaat
     5581 ttgctgttcg ccgccgatta aattgacctc ataatatccc cagtcactga aatgttccgt
     5641 agcaagaata tcggcataca caccctgatg cgcacggtga aatgcaccaa agcctaaatg
     5701 aacaattctt ggggccaggt tattacgatc ataaacaggg agcgtcgctt ttgctgataa
     5761 caaattattt cccataacaa ttccttaaat ttaaatatgg caagctgcat gttttgttat
     5821 atgaataaaa aatcccctct ccggtaagag aagggatgga ggatttacag acttcttgaa
     5881 ggttgcgcag ctgttacaac acgcggttga tcttccgcgg catcttccag tgcacttaaa
     5941 tcacggtctt tcacttctgg cattttcagc gcagagatta aaccaatcac tgaatatgcc
     6001 atgatcataa tggcgatcgg ataccaggat tccgtcatgg tgcagaaaat acccgccagg
     6061 ataggaccaa aaccggaagc gataagacca ccaatttctt tagaaatggc catccgggta
     6121 aagcggtttt tacagccgaa catttctgcc atggtaatgt tttccagagc aaataatccc
     6181 agcaccgcac agttatgaat cacaatcagt gccaccataa tggtgctcgg ggcatagctt
     6241 ttatctacga tgatagaaag cattggccat gccagcacaa tcgcggaggt attcataata
     6301 atatacggga tccggcgacc aattttatcg gataaccaac caaggaacgg aatggtcata
     6361 aagccgagaa tcgaactgat catcaatgca tctgttggaa ttgctttgtt aaacaataac
     6421 gtctgtacta aatagcctgc aaggaaagtc tgaattaaac cggagttacc agcctgacca
     6481 aaacgcagcc ctgttgccag ccagaaggat ttgctctgga acatgctacc agcaggtgca
     6541 ggttttgctg tcggttggtt gctgtcgtta accttctcaa agaccgggct ttctttcaga
     6601 ttcatacgca accagatagc aaagaccatc acgacaacgc tcgccaggaa cggtatacgc
     6661 catccccacg ccaacagttc ctctttactg agaatgaaga acataaaggc ccagattgcc
     6721 gttgcgctta aggttccgca gttagttccc atagccacaa atgaggagat aattccgcgc
     6781 ttaccttttg gcgcatattc cgccagcatc gtaccggcac cggaaatttc cgcacctgca
     6841 cccaatccct gaataatacg cagcgtcacc agcaagatgg gtgcaaaaac accaatctgt
     6901 gcataggtcg gtaacacacc aattaaggtg gtacagatcc ccatcatggt gatggtaata
     6961 aagagtactt ttttacgccc tattctgtcg cccattttgc cgaaaataaa tgctccgaca
     7021 atacgcgcca cataacctgc accgtaggtt cccattgcca gaattaacgc cattgccgtt
     7081 gatgattcag gaaaaaatat ttcatgaaac actaacgctg caccgagcga atataactgg
     7141 aaatccataa attcaagcgc agtgcccagc catcccgata ctgctgcttt caccaaatcc
     7201 ttagtgcttc tttcgtgttt ttctattgtc ataatggtta tctctaaaaa agaggtaaga
     7261 tgcgtactac ttactcgccg ttattggtat tattcagaaa aagtaagtaa gactttgcag
     7321 caatgttttt gatcctgttc aaataaacta atggcatcag caacatgctg gaaatcaaac
     7381 gtatgggtaa ttaatttttc tggtttaatt aaccctttac ttaaccagtc gataacgacc
     7441 ggaaatttat ttgcatttaa gcgtgaagag aaaatagaga gttcttttcc ggtaattcct
     7501 tgctgaatca cttcagacgg ttcactggag aagcccatca atacaatacg tgccgctgga
     7561 gaagccagcg ttacagcctc tttcaggata gaaggatgac aagccgcatc gataattaat
     7621 gtcggcttga tgcctttttc agcgaaactc tcgccaagcg gtgtctggct gttattaatc
     7681 gcccagtctg ccccgctctc tttcgctttt tccagtcgtt catcaatgcg atcggcaaca
     7741 atcacatttt taacgttata gacgcctttt aatacctgaa cgatcgtcag gccgattgga
     7801 ccggcaccgt aaaccagaac ggtatcattt tcagtcggtt gaccatgacc ggtaacgtta
     7861 gccgcaatgg taaaaggttc gatcatcacc gcatattgat cggccactgc ttcaggaatt
     7921 ttccacgcat tttttgccgg aaccacggca tattcactga aaccaccgtc agcgtgcaca
     7981 cctaatacag ccagtgtcgt acaaacgttc ggcttaccta tagagcacgg atagcaatgc
     8041 ccacagctga ccaccggatc gacagcaacg cgttcaccga ctctggcgct ttccacgcct
     8101 tcacccactg catcaatgac gccaaagaat tcatgaccaa tgacgcgcgg atatttcgca
     8161 aaaggattat gcccacgata aatatggcta tctgaaccac aaattccggc aagtttcact
     8221 tttactcgta cttcacccgc tgacggggtg ggtatttcgc gttcgataat tgccagttga
     8281 ttcggttttt caattaaaat gcttttcatt atcttactcc ttaccagttc cacagcgtgc
     8341 catcttccag acgtgcgact ggcagataag caggttcata gggatatttc gccgccagct
     8401 tttcatcgaa ttcgatgcca agacccggtt tgtctcccgg atgcatatag ccgttatcga
     8461 aagtccagtt gtgcgggaag acttcaagca tttgttcgga ataacccatg tactcctgga
     8521 caccgaaatt ggggacccac agatcaaagt gcagcgccgc agccatgcag actggcgaca
     8581 aatcggaagg accgtgtgag ccagtacgta cctgatacag cgaagcaaaa tcggcaatcc
     8641 ggcgcatacc ggtaatcccc cccgcgtgag tcagcgtggt gcggatatag tcgataagtt
     8701 gttcttcaat cagctgtttg cagtcccaga tgctgttgaa gacttcaccc actgcaattg
     8761 gcgtgacggt gtgttggcga atcaggcgga aacactcctg gttttccgca ggcgtcgggt
     8821 cttccatcca gaacatgcga taatcttcaa tgcttttacc aaagcgcgcc gcttcaatag
     8881 gcgttaagcg atggtgcatg tcatgcagca aatgttcatt aaaaccaaac ttgttacgta
     8941 ccgcgtcaaa caatttcggc atgaaatcga ggtatttctc cgtcgaccac agctgctctt
     9001 ccggccactg tcctttggtt gcgggttcat aagccagacc tttacctttc gacatgccat
     9061 aggtggtttt cataccaggg attccgcact gcacgcggat ggctttgaat cccagctcct
     9121 gatgacgggc ataatcatcc agagcttcat caatactgtg accggtggta tggcaataaa
     9181 ccatcacccc ttcacgagac gcgccgccga gtaactggta aagcggcatg ttggcagctt
     9241 tggctttaat atcccacagc gccatatcaa ccgctgaaat ggccgacatc gtaaccggac
     9301 cgcgacgcca gtaagcacct ttatagaaaa actgccagat atcttcgata cggtgtgcat
     9361 cgcgaccaat aagctgcgga caaaggtgat cctgcaaata agaggccacg gaaagctcac
     9421 gtccattgag ggtggcatcc ccaaggcccg taataccgtc ctcagtggtg atttttaatg
     9481 tgacgaaatt acgccccgga caggtaacaa aaacttcagc ctttacgatc ttcatgttcg
     9541 attccttgca tcgcttgtcg tgatgcatga aatctacgca actgagctac taccatacaa
     9601 gtatgaagat caaaaaaagc cggagtgatc acaaaaaaag gcgtatattt gcgccgtgaa
     9661 tggttgacaa aagatgaaat agaatacctt ttgtcagctg acacttcctc gtatcttatt
     9721 gataaaaggg atttatgttc ctacgtgcgc ccccagcccg caacaatgat caacatgccg
     9781 caaagcgcaa tcaacgcccc cgtccagtca taaagactca gtttcacgcc atccacaacg
     9841 cgcagccaca tcaacgccgt gcagacataa acgccaccat aagcggcgta aacacgccca
     9901 ctcgccgcag gatgcaacgt taacaaccag acaaacagcg ccagtgaaat ccccgccgga
     9961 agcaacagcc agatactggc gtttcgtttt aaccacaacc agggcagaaa gcatccaata
    10021 atttcacaca gcgcagtagc aaaaaatagt aacgttgttt taatcatctt tgtcacttat
    10081 tgacatcatg tatagttata ggacgacata atatcatcaa tataaacatc ctcctggtac
    10141 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
    10201 tatgaaaatt actctcagca aacgaattgg cctgctcgct tttctgctgc cttgcgcact
    10261 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
    10321 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
    10381 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
    10441 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc caaatacttt
    10501 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
    10561 gaatgtaagg acacgttatg ccaagcgccc acagtgttaa gctacgcccg ctggagcgtg
    10621 aagatttacg ctatgtacat caactcgaca ataacgccag tgtgatgcgt tactggtttg
    10681 aggaacccta cgaagccttt gttgaactct ctgatctgta cgataagcat attcacgatc
    10741 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
    10801 tggaaattaa ccatgttcat cgccgcgcag aatttcagat aattatctcc ccggaatatc
    10861 aggggaaagg tctggcaacg cgtgccgcaa aattagcaat ggactatggc ttcaccgttc
    10921 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
    10981 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagttcttt attaatggtc
    11041 aatatcgtaa tgccattcgc atgtgcatat tccagcatca gtatctggca gagcacaaaa
    11101 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatagtat
    11161 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgattagct
    11221 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaaccc
    11281 tttgattatt cagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
    11341 gcgtagcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
    11401 cctgatcatc atagcgatat tccatctgca tctgtttgct gctggcttta atcacaaagc
    11461 cattatcatc ggtttcccag ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
    11521 cctgcaaacg aactctcttc tccagtgtct gggcatcacg gtaataattg gcgtccagta
    11581 ccagggcaac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
    11641 cttccgacaa agtccccgaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
    11701 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
    11761 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
    11821 cgagtaagca tggcagtaat ttgtatttca caacagtcac cagagagtag agatgattct
    11881 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
    11941 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
    12001 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
    12061 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
    12121 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
    12181 attctctaca cccgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
    12241 ggcgcgcctg ccgaaccgca gtaatacatt atcgactgca cgccggatac gacacatccg
    12301 gcgatttgaa catacaacat aatcccacct tattactcat acccttctat tgatatggat
    12361 taataattct taacccaaaa taggtagact ccctctattg ttagcgcgct aaatattcaa
    12421 tatataaact tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaacaa
    12481 atggtgggca tcagccgcag aacactcgtt aaatctaccg cgataggttc tctggcgctg
    12541 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacagcag
    12601 gccagcgaga aagtggtctg gggtgcctgt tccgtcaact gtggtagccg ctgtgcactt
    12661 cgtctgcatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaacac cggcagcgat
    12721 gagtatggca atcatcaagt acgcgcctgt ttgcgcggtc gctccatccg tcggcgtatt
    12781 aatcatcccg accgcttgaa ttacccaatg aaacgcgtgg gcaaacgcgg cgaaggcaaa
    12841 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
    12901 gtcgaacaat atggcaatga ggctgtatat attcagtact cttcagggat cgttggcggc
    12961 aatatgaccc gctcttcgcc atccgcctcg gcggtcaaac gcctgatgaa ctgctacggc
    13021 ggttcactca accagtatgg ctcctacagc accgcgcaaa tctcctgtgc catgccctac
    13081 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
    13141 atgtttggta acaacccggc tgaaacccgc atgagcggcg gtggcatcac ttatcttctt
    13201 gaaaaagcgc gcgagaaatc gaacgcaaaa atgattgtta tcgatccgcg atataccgat
    13261 acggctgctg gtcgtgaaga tgaatggctt cctattcgcc cgggcaccga tgccgcgctg
    13321 gttgcgggta ttgcctgggt attggttaac gaaaatctcg ttgatcaacc ttttctcgat
    13381 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
    13441 tataaagcct atattcttgg tgaaggtgat gataaaacag cgaaaacgcc gcagtgggct
    13501 tcgcaaatta caggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
    13561 gcaaaacccg cttatatctg ccagggctgg gggccgcaac gccaggcaaa cggcgagctg
    13621 acggcacgcg ctattgctat gttgcctatt ttgacgggca atgtcggcat cagcggcgga
    13681 aatagtggtg cgcgtgaatc gacctacacc attaccatcg aacgcctgcc ggtgctggat
    13741 aatccggtca aaacgtcaat ctcctgcttc agttggacag atgctatcga tcatggcccg
    13801 caaatgacgg caatccgcga cggcgtccgg ggcaaagata aactggatgt gcccattaag
    13861 ttcatctgga actacgcggg aaataccctg gttaatcagc attctgacat caacaaaacg
    13921 catgaaattc tgcaggacga atcgaaatgc gaaatgattg tggtcatcga aaactttatg
    13981 acctcatcgg caaaatatgc cgacattctg ctgccagacc tgatgaccgt tgagcaggaa
    14041 gatattattc ctaacgacta cgccggtaac atgggatatc tcattttcct ccagcctgtc
    14101 accagcgaaa aattcgaacg caaaccaatt tactggatcc tgagtgaagt cgcgaaacgt
    14161 cttggacctg acgtctatca aaagttcact gaaggtcgca cgcaggaaca atggttacaa
    14221 catctgtacg ccaaaatgct tgccaaagat ccggcgttac cgtcttacga cgaattaaaa
    14281 aaaatgggta tctataagcg taaagatccc aatggtcatt ttgtcgccta caaagcattt
    14341 cgtgacgacc ccgaggcaaa tccacttaaa acgccttccg gtaagattga aatttattcc
    14401 agcaagctgg cggaaattgc ccgtacctgg gaactggaaa aagatgaagt gataagccca
    14461 ctgccggttt atgcctcaac ctttgaaggc tgggactccc ctgaacgtag tacctttcca
    14521 ctgcaactgt ttggtttcca ttacaaatcc cgtactcact cgacctacgg caacattgat
    14581 gtcctgaagg ctgcctgccg tcaggaggtg tggatcaacc ctatagacgc acagaaacgt
    14641 gggattgcca acggcgatat ggtgcgggtg tataaccatc gtggcgaagt tcggctacca
    14701 gccaaagtga caccacgcat tctccctggg gttagcgcta tgggccaggg agcctggcac
    14761 gaggccaata tgtctggcga taaaatcgac catggcggct gtgtgaatac gctaaccact
    14821 ctgcgccctt caccactggc gaagggaaac ccgcagcaca ctaatctggt cgagatcgaa
    14881 aaaatataac ccactacaac cataaattct ggcatgacat tttgtttgaa aagcaataag
    14941 tgagtaatga tgaaaatcca taccacagag gcgctcatga aggctgaaat cagccgtaga
    15001 agtctgatga aaacctccgc acttggcagt ctcgcgctgg caagcagtgc tttcactctg
    15061 ccattttccc aaatggtccg agcggcagag acaccggtag aagagaaagc ggtctggagt
    15121 tcctgcaccg ttaactgcgg gagccgctgt ctgttacgtt tgcatgtgaa agatgacacc
    15181 gtgtactggg tggagtctga tacgacagga gacgacgttt acggtaatca tcaggttcgg
    15241 gcatgtttac gcgggcgctc tatccgccga cggatgaatc atcctgatag gttgaaatat
    15301 cccatgaagc gcgtcggcaa gcgcggtgaa ggtaaatttg aacggataag ttgggatgaa
    15361 gccctggata ccatcagtga taatcttcgg cggatcctta aagattacgg caatgaggct
    15421 gtacatgtcc tgtacggaac aggcgtagat ggcggaaaca tcaccaactc gaacgtcccg
    15481 taccgtctga tgaactcttg cggtggtttt ctcagtcgct atggcagcta cagtaccgct
    15541 caaatcagtg ccgcaatgag ttatatgttc ggtgccaatg atggcaacag cccggatgat
    15601 atcgccaata cgaaactggt cgttatgttc ggaaataacc cggcagaaac ccggatgagc
    15661 ggcggtggtg tcacttacta cgtcgagcaa gcccgagaac gttcaaacgc acgcatgatc
    15721 gtcatcgatc cacgttataa cgacacagct gccgggcgtg aagatgaatg gctgccaatt
    15781 cgcccaggca ccgatggcgc actggcctgt gcgattgcct gggtactgat tactgaaaac
    15841 atggtcgatc agccatttct cgacaaatat tgtgttggtt acgatgaaaa aacgctgccc
    15901 gccaacgcac cacgtaacgc gcattataaa gcctatattc tgggcgaagg gcctgacggc
    15961 atagctaaaa cgccggaatg ggcagcaaaa atcaccagca tcccggcaga aaaaattatc
    16021 cagttggcac gagagatcgg ttcagcaaaa cctgcttata tttgtcaggg ttgggggcca
    16081 caacgacact ccaacggcga acaaacgtcc cgcgctattg ccatgctttc cgttctcacc
    16141 ggtaacgtcg gcataaacgg cggcaactca ggtgtacgcg aaggtagctg ggatctgggg
    16201 gtagaatggt tcccgatgct tgagaatcct gttaaaacgc agatttccgt ctttacatgg
    16261 acagatgcca tcgaccacgg tacggaaatg accgcgaccc gtgatggtgt tcgtggaaaa
    16321 gaaaaactgg atgtccccat caagttttta tggtgctacg ccagtaacac attgatcaat
    16381 caacatggcg acatcaatca cacccatgag gtgcttcagg atgacagcaa gtgcgagatg
    16441 attgttggca ttgaccactt catgactgcc tcggctaagt attgcgatat cctgttgccc
    16501 gacctgatgc caacagagca agaagacctt atctctcacg aatctgccgg gaatatgggc
    16561 tatgtgatcc tcgcccaacc cgcaacctca gcaaaatttg aacgcaaacc catctactgg
    16621 atgctgagcg aagttgccaa acgcttagga ccagacgttt atcaaacctt tactgaaggc
    16681 cgcagtcagc acgaatggat caaatatctc catgcgaaaa cgaaggaacg taaccctgag
    16741 atgcccgact acgaggagat gaaaacgacc gggatcttta agaaaaaatg cccggaagaa
    16801 cactacgtcg ctttccgcgc attccgtgaa gatccacagg caaacccgtt gaaaacacct
    16861 tcggggaaaa tcgaaattta ttctgaacga ctggcgacga ttgcggatac ctgggaattg
    16921 aaaaaagatg aaattattca tccccttcct gcgtataccc caggttttga tggctgggac
    16981 gatcccctgc ggaaaaccta tccactgcag ctaacgggct tccactacaa agcgcgtacc
    17041 cactccagct acggcaatat tgatgtgtta cagcaggcct gcccacaaga ggtgtggatc
    17101 aaccctattg atgctcaggc acgcggtatc cgtcatggcg ataccgtgcg ggtatttaac
    17161 aataatggag aaatgctgat tgccgcaaaa gtgactccgc gtattctgcc tggcgttacc
    17221 gccatcgggc aaggtgcgtg gcttaaagcg gatatgtttg gtgaccgggt cgatcacggc
    17281 ggcagtatca atattctgac ctctcaccgc ccttcaccgc tggcaaaggg aaacccgtcg
    17341 cacagcaatc ttgtccagat cgaaaaggtt taaggagtag cccatgacca cacaatatgg
    17401 attttttatc gattccagcc gctgtactgg ctgtaaaacc tgcgaactgg cgtgcaaaga
    17461 ttttaaagat cttggcccgg aagtcagttt ccgccgtatt tacgaatatg ctggcggcga
    17521 ctggcaggaa gataatggcg tctggcacca aaacgtgttc gcttattatc tctccatctc
    17581 ctgtaaccat tgcgatgacc ccgcatgtac aaaagtctgc cccagcggcg caatgcataa
    17641 gcgtgaagat ggctttgtgg tggttgatga ggatgtttgt atcggctgcc gctactgcca
    17701 catggcttgt ccgtacggcg cgccacagta caatgctgaa aaagggcaca tgacgaagtg
    17761 cgatggttgt tattcgcgcg tcgccgaggg gaaacaaccc atatgtgtcg aatcctgccc
    17821 gctgcgtgca cttgagttcg gtccaattga agagctgcgc cagaagcacg gcactctggc
    17881 tgccgtcgcg ccactaccgc gcgcgcattt cacaaaaccc aatatcgtta tcaaacctaa
    17941 cgccaacagc cgcccgaccg gcgataccac tggttatctg gctaatccgg aggaagtgta
    18001 aaatgggaaa tggatggcat gaatggccac tggttatctt taccgtattg ggtcaatgcg
    18061 tagtaggcgc gctgattgtt agcggaattg gctggttcgc cgccaataat gacgcagacc
    18121 gtcagcgtat tgtccgtggc atgtttttcc tctggctgtt gatgggcatc gggtttattg
    18181 cctctgtcat gcacctggga tcgccactgc gagcgtttaa ctcgctgaat cgaatcggag
    18241 cttctggtct gagtaacgag attgctgccg gttccatatt ttttgccgtt ggcggattat
    18301 ggtggctggt ggcagtcatc ggtaaaatgc cgcaagcgtt ggggaaactc tggttacttg
    18361 tcagcatggc gctcggcgtc attttcgtct ggatgatgac ctgcgtgtat caaatcgaca
    18421 cagtgccaac ctggtataac ggttacacta cgctggcttt cttcctgaca gtattgttga
    18481 gcggtccaat acttgctgcc acaattctgc gggcagcccg cgttacattt aataccacac
    18541 catttgccat cattagcgtt ctggcgctga tcgcttgtgc cggggtgatt gtgctgcaag
    18601 gtttgtctct ggcgtctatt cactcatccg tacaacaagc cagcgcactg gtaccagatt
    18661 acgcctcctt acaggtctgg cgtgtggtat tgttatgcac aggtctagga tgctggcttt
    18721 gtccgctcat tcgtcgccgt gaacctcacg ttgccggact tattctgggg ctaatcctca
    18781 tacttggcgg tgagatgatc gggcgtgtgc tcttttatgg cctgcacatg acggtaggaa
    18841 tggctattgc aggttaacaa attggtgcgg ggcaacccgc acatttcagg atgttaggaa
    18901 tgacccattt ttcacagcaa gataattttt ctgtcgcggc gcgcgtgttg ggcgcgctgt
    18961 tttattacgc tccagagagt gcggaagccg cacctctggt tgcggtactc acccgtgacg
    19021 gctgggaaac tcagtggcct ctaccagaag cgtcattagc gccgctggtg acggcttttc
    19081 agacacagtg tgaagagact cacgcccagg cctggcagcg tttgtttgtc ggcccgtggg
    19141 cactgccgtc tccgccgtgg ggatcggtct ggctggatcg cgaatctgtg ctgtttggcg
    19201 attcaacatt ggcacttcgt cagtggatgc gcgagaaagg cattcagttt gaaatgaagc
    19261 aaaacgaacc ggaagatcat tttggttcac tgctgttgat ggctgcgtgg ttggcggaga
    19321 atggtcgcca gacagaatgc gaagaattgc tggcatggca cctttttccg tggtcaacac
    19381 gatttcttga cgtttttatc gaaaaagcag aacacccttt ctaccgtgca ctgggggaac
    19441 tggctcgctt aacgctggcg caatggcaat cacaactgtt gattcctgtc gcggttaaac
    19501 cactctttcg ataggatcac cgtaatattg ccggatggtg gcgattacgc gcaattcggc
    19561 aataataggt tacagtgtca cgttttttta tctcttaaag cacgcactgc ttttgcggct
    19621 ggcctctttt gccgcaaaat agtcgcccgt gtttcattgc ccatttctgc tcatgcatca
    19681 tctacacatc tatccggatc tgcgcactat gttccgccgt ctgcttatcg caacagtcgt
    19741 cggtattctt gcggcctttg ccgttgccgg gtttcgtcat gcaatgctgc tactggagtg
    19801 gttgttcctc aataatgact cgggcagtct ggtcaatgca gcgacaaatc tttccccatg
    19861 gcgacggctg ctaactccgg cgctcggcgg actggcggcg ggtttgttgc tgatgggctg
    19921 gcagaaattt acccagcaac gccctcatgc gccgaccgat tacatggaag cgttgcaaac
    19981 cgatgggcag tttgattacg cagcaagcct ggttaaatcg cttgcctctc tgctggtagt
    20041 aaccagcggc agtgcaattg gtcgcgaagg tgcgatgatt cttttagctg cccttgccgc
    20101 ctcctgtttt gcccaacgtt ttacgccacg ccaggagtgg aaattatgga tcgcctgtgg
    20161 agctgcggcg ggaatggctg cggcctatcg cgccccgctt gctggcagtt tatttatagc
    20221 cgaagtgctg tttggcacta tgatgttggc ctctctcggc ccggtgatta tttccgccgt
    20281 cgtggcgttg ctggttagca atctgattaa tcatagcgac gcgttactct acagcgtaca
    20341 actctcagtg acggttcagg ctcgtgacta tgcgctgatt atcagtacag gtgtgctggc
    20401 aggtctgtgc ggaccactgt tgttaacgtt aatgaacgcc tgtcatcgtg gatttgtaag
    20461 tctcaaactt gcgccgccct ggcaactggc actgggcggg ttgatcgtgg gtctgctttc
    20521 cctgttcaca cctgcagtgt ggggcaacgg ctatagcacc gtacaatcct ttttaaccgc
    20581 cccgccactg ttaatgatca ttgccgggat cttcctttgt aaactgtgtg ccgtgctggc
    20641 gagtagcggt tccggcgcac ccggtggtgt ctttacaccg acgctattta tcggtcttgc
    20701 cattggcatg ttgtatggtc gtagcctggg attatggttc cctgatggcg aagaaattac
    20761 gcttttactc ggattgaccg ggatggcgac actgttggcg gcaaccacgc acgcgccgat
    20821 tatgtcgacg ttgatgatat gtgaaatgac cggggagtat cagctactcc ccggtttatt
    20881 gattgcctgc gtaattgcgt cggtaatttc gcggacgtta caccgtgact ctatctaccg
    20941 ccagcacact gcgaagcata gctaagcgga tgtattgccc cagttcacgc tgttcggcgc
    21001 gcggcagata aggcagttca ccaatgagcg gtgccggaag ttttttaccg agcacatcaa
    21061 tgatttccgc ataatgcgcc agtcctggat tgattcggtt agccacccag ccaatgagcg
    21121 gcagcccgtc gttggcaatc gcctgagctg ttagtagtgc atggttaatg caaccttcct
    21181 gaataccgac aaccatcaat accggcagtt gttcctgcac cacccattca gagagcggac
    21241 gcaaatcatt catcagactg cgccagccgc cagtcccttc taccacgaca tgatcgactt
    21301 tatcggtcag gtttgccagg ccgtttgaaa tgagggtgta attgattggg caactgtgcg
    21361 ccacgctact ttcttcttcg cttaacgcga taggattaac tgcttcataa ggcagttcga
    21421 tggttgaaac actctgcaac accagggcat ctttattacg cagcccttcg ggtgtctctt
    21481 tgctcccctt cgctacgggt ttatatcccg caaccgtttt tccctgggag gctaacgctt
    21541 gtagcaatgc gcgggaaacc accgttttcc ctacagaagt gtctgtaccg gtaataaaga
    21601 aacgcttcag catcactaac tccaccgtta tgcttcacaa atataaacca ggaaaataat
    21661 taaccttgaa agtctaagtt atgctttcct ggcccaaatt gagatagcgc aaattttggt
    21721 agaacagtta aaaaatgtta accctgcaac agacgaatca acaaagaacc gttatacatc
    21781 gcgtctttta ccagcgcagc ccctgccatc gtaccctggt tagaaaactg agtactctca
    21841 acgctaatgt gctgactata cgcaggaagg gcctgctgac ggatgctgtc cgagatgacc
    21901 gggaagagga tatctgccgc tttacttaac ggtgagccaa tcagtatttt ttgtgggtta
    21961 aataaattca ccatgatggc aagaatgcgc cctacatgcg cgcccacccc ggtaatgatg
    22021 tcttttgcca gtagatcgcc gcgcaatgcc gcctgacaca atgaatccac ggttaacggc
    22081 tgtccatgta acatcgagct catggattga ttgagacgca gctgtgccag ctcaagaata
    22141 ctgtccacac tggcgatggt ttcgaggcag ccgtgattcc cgcaataaca gcgtttccca
    22201 tacgggtcga cctgcgtgtg acctatttcc acgaggctac tgctgcctgc gtgtagcaga
    22261 tgaccatcgg taatgacgcc cgcccccacg ttgtgatcga taaccacctg aatcacatcg
    22321 cgcgccccgc gtgaggcacc aaacaaggcc tcagccatcg tccatgcgct gatatcatgc
    22381 tgaatataaa ccggtacgcc gatatgctgc tccagcgcct cgccgagcgg catctctttt
    22441 acatcctcgt agaacggcat gcgatgtaca ataccatttt ccgtatcaat aattcccggc
    22501 aaggttatgg caatcgaagt cagacgctca agttttttct ggtggcggat aaaaaactga
    22561 tcgatatggg aaatgatacg atccagcaat ggcgagtcat cttttaacgc cagttcctgc
    22621 gactcctcca ctaccagttt gctgctcaga tcgcgcaaag caaggaaaat ctccccgcga
    22681 ctaatgcgca gagaaagata gtgccaggct tcagtttcaa ccaccagccc cactgccgga
    22741 cggccacggt tccccgcttc tttgatttcc agctcttgca ccaggtgtgc ttcgagcatc
    22801 tcacggacaa ttttagtgat actggcagga gccagctgcg ccagacggga aagatcgata
    22861 cgcgagactg gaccaagctg atcaatcagg cgataaaccg cgcccgcgtt ggtctgcttt
    22921 atttgatcaa tgtgcccagg ctgattttca gcaaccaccg catactccct atattttcgc
    22981 gctccgaaat aatctgtagg ctatggtgaa gcacttcaat acgtgtcgtc aaatttttac
    23041 ttaggcatgt gattaacagc acatttttcg ggctttttcg ctgaaatttc cctcacctga
    23101 gagcattcag cagatgaata cgaaagttac gcgcagccgg actttgttca tggtgtttcg
    23161 gccagaccaa ccacatttca gaaaccgcat cctcttcagc aatcggcacc cagcgcattt
    23221 cgttgagctg aacacgttta aatgacgcag gcaaaattga aacacccaga ccagcggaaa
    23281 ccagaccgat gatggtcatt gcctcaccca cctcctgagt gatgacgggc gtcaaattgt
    23341 aacgtcgcat cagcccgaga atatcgtcat acagccctgt ccctacgtgc ggatcaaaaa
    23401 agacaaaggg ttctttcgcc agttcagcca gcgttacact cgggttattt gccaagggat
    23461 gatcgtgcgg gatcatcgcc ataagcggtt catggacgat gactgcgtgt tcaagcgtct
    23521 ccggtaacgc tgtgttacgc agcaatccca tatccagcgt tccttcaatg agcggagcga
    23581 tttgctcgcg ggtgttcatt tcgcgggttt gtaaatggac atcgggataa tcacggcgaa
    23641 acagcgataa cgtatcggac accgcccgaa taaaaggagc ggacgaagta aaaccaatgc
    23701 gcaactcccc cgcttcaccc tgatgcagtc tttcagcgcg agcggcagcg tcatccatca
    23761 tagacaggat ttgccgacta tctgcaagaa actgttttcc tgctgccgtc agcaatacac
    23821 tgcgattagt tcgtgccagc agtcgggcac caatttgttg ctccagcgcc tgaatctgct
    23881 gacttagcgg cggttgcgaa atattcaggc gggcagcggc gcgcccgaaa tgcagctctt
    23941 ctgcaacagc aacaaagtaa cgcagatgac gaagttcaat attcatattt aa
//
LOCUS       DAZGAP010000076        23739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGAP010000076 DAZGAP010000000
VERSION     DAZGAP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.076"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            199..1713
                     /gene="caiT"
                     /locus_tag="ACHLAM_002516"
     CDS             199..1713
                     /gene="caiT"
                     /locus_tag="ACHLAM_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO7854027.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1744..2886
                     /gene="caiA"
                     /locus_tag="ACHLAM_002517"
     CDS             1744..2886
                     /gene="caiA"
                     /locus_tag="ACHLAM_002517"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO7854028.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3014..4231
                     /gene="caiB"
                     /locus_tag="ACHLAM_002518"
                     /pseudo
     CDS             3014..4231
                     /gene="caiB"
                     /locus_tag="ACHLAM_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            4304..5857
                     /gene="caiC"
                     /locus_tag="ACHLAM_002519"
     CDS             4304..5857
                     /gene="caiC"
                     /locus_tag="ACHLAM_002519"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO7854029.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5966..6751
                     /gene="caiD"
                     /locus_tag="ACHLAM_002520"
     CDS             5966..6751
                     /gene="caiD"
                     /locus_tag="ACHLAM_002520"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO7854030.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEAQRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6757..7347
                     /gene="caiE"
                     /locus_tag="ACHLAM_002521"
     CDS             6757..7347
                     /gene="caiE"
                     /locus_tag="ACHLAM_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO7854031.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAIIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYK
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7466..7861)
                     /gene="caiF"
                     /locus_tag="ACHLAM_002522"
     CDS             complement(7466..7861)
                     /gene="caiF"
                     /locus_tag="ACHLAM_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO7854032.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8122..11343)
                     /gene="carB"
                     /locus_tag="ACHLAM_002523"
     CDS             complement(8122..11343)
                     /gene="carB"
                     /locus_tag="ACHLAM_002523"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO7854033.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11361..12509)
                     /gene="carA"
                     /locus_tag="ACHLAM_002524"
     CDS             complement(11361..12509)
                     /gene="carA"
                     /locus_tag="ACHLAM_002524"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO7854034.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12966..13787)
                     /gene="dapB"
                     /locus_tag="ACHLAM_002525"
     CDS             complement(12966..13787)
                     /gene="dapB"
                     /locus_tag="ACHLAM_002525"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO7854035.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(13954..14868)
                     /gene="rihC"
                     /locus_tag="ACHLAM_002526"
     CDS             complement(13954..14868)
                     /gene="rihC"
                     /locus_tag="ACHLAM_002526"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO7854036.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(14934..15884)
                     /gene="ispH"
                     /locus_tag="ACHLAM_002527"
                     /gene_synonym="lytB"
     CDS             complement(14934..15884)
                     /gene="ispH"
                     /locus_tag="ACHLAM_002527"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO7854037.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15886..16335)
                     /gene="fkpB"
                     /locus_tag="ACHLAM_002528"
     CDS             complement(15886..16335)
                     /gene="fkpB"
                     /locus_tag="ACHLAM_002528"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7854038.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16443..16937)
                     /gene="lspA"
                     /locus_tag="ACHLAM_002529"
     CDS             complement(16443..16937)
                     /gene="lspA"
                     /locus_tag="ACHLAM_002529"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO7854039.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16937..19753)
                     /gene="ileS"
                     /locus_tag="ACHLAM_002530"
     CDS             complement(16937..19753)
                     /gene="ileS"
                     /locus_tag="ACHLAM_002530"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO7854040.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSLSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCANAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19796..20737)
                     /gene="ribF"
                     /locus_tag="ACHLAM_002531"
     CDS             complement(19796..20737)
                     /gene="ribF"
                     /locus_tag="ACHLAM_002531"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO7854041.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20745..20963)
                     /gene="yaaY"
                     /locus_tag="ACHLAM_002532"
     CDS             complement(20745..20963)
                     /gene="yaaY"
                     /locus_tag="ACHLAM_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO7854042.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            21066..21329
                     /gene="rpsT"
                     /locus_tag="ACHLAM_002533"
     CDS             21066..21329
                     /gene="rpsT"
                     /locus_tag="ACHLAM_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO7854043.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(21425..22330)
                     /gene="nhaR"
                     /locus_tag="ACHLAM_002534"
     CDS             complement(21425..22330)
                     /gene="nhaR"
                     /locus_tag="ACHLAM_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO7854044.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(22390..23556)
                     /gene="nhaA"
                     /locus_tag="ACHLAM_002535"
     CDS             complement(22390..23556)
                     /gene="nhaA"
                     /locus_tag="ACHLAM_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO7854045.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         5791 a         6362 c         6010 g         5576 t
ORIGIN      
        1 caaaattaac gaaaaaatgc cctctattca cccgaaacaa aaatgtgata acaatcacag
       61 aatgcagctt attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa
      121 taattatatt aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa atgttcgttt
      181 tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt
      241 ttctttccgc cgttaataat cgtcggcata ctttgttggc ttacagtcag agatctggat
      301 gcggcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca
      361 tttgaatggt atatggtggt gatgcttttc ggctggttct ggctggtgtt tggcccgtat
      421 gccaaaaagc gtttaggtaa cgaaccacca gaatttagta ccgccagttg gatctttatg
      481 atgttcgcct cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac
      541 tacatctcca ccccgccgtt tggcttagaa ccgaactcaa caggcgcgaa agagttgggg
      601 ctggcttaca gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca
      661 gtcgccttcg cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctccacc
      721 ctggtgccgc tggtaggtga aaaacacgcc aaagggttgt tcggcactat cgtcgacaac
      781 ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg
      841 gtgaccgagt gtatgcaatg gttgtttggc attccgcata ccctgcaact ggacgctatc
      901 atcattacct gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg
      961 gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc
     1021 atcgtcagcg gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg
     1081 atgtatctgc cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag
     1141 ggctggaccg tgttctactg ggcatggtgg gtgatttacg ctatccagat gagcatcttc
     1201 ctcgcccgca tctcccgtgg tcgtaccgtg cgtgaactgt gcttcggcat ggtgctgggg
     1261 ctgacagcgt caacctggat cctgtggact gtactcggta gtaacactct gctgttgata
     1321 gataaaaaca tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc
     1381 atcgaaacct gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc
     1441 tgctttattg ccaccgttac gctggttaac gcctgctctt ataccctggc gatgtccact
     1501 tgccgcgaag tacgcgatgg tgaagaacca ccgctgctgg tgcgtatcgg ctggtcaatt
     1561 ctggttggca ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc
     1621 gccattatcg ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt
     1681 attaaagacg cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga
     1741 aagatggatt ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg
     1801 atggccagcg aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg
     1861 gaacgttttg tcaaagcact ggcggatatg ggtatcgaca gtctgctgat tcctgaagag
     1921 cacggtggtc ttgacgcggg ttttgttact ctcgccgccg tgtggatgga gctgggacgt
     1981 ctgggggcac caacctatgt gctgtaccag ttgccgggcg ggttcaatac cttcctgcgc
     2041 gaaggcacac aagagcagat cgacaagatt atggctttcc gcggcaccgg taagcagatg
     2101 tggaactcag cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact
     2161 tatacccgta gaaatggtaa gatttatctt aatggtagta agtgttttat taccagcagc
     2221 gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc
     2281 tacaccgaat ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgagaag
     2341 ctcggtctgc gtatggatag ctgctgtgaa atcacttttg acgacgtgga actggacgag
     2401 aaagacatgt tcggtcggga aggtaacgga tttaaccgcg tcaaagaaga gttcgaccat
     2461 gaacgtttcc tggtagccct gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg
     2521 gcgcgctacg ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt
     2581 caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa
     2641 gcagcgtgga aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac
     2701 ttctgcgcca atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc
     2761 gggattgcgg gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt
     2821 tccggaggat ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct gaagcaatac
     2881 cgttaagtcc gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca
     2941 cgaagatggc cggataaggc gtttacgccg catccggcag tcatgcgcta cataaatttt
     3001 tcaggagcta ataatggatc atctacccat gccgaaattc gggccgctgg ccggattacg
     3061 cgttgtcttc tccggtatcg aaatcgccgg gccgttcgcc ggacaaatgt tcgcagaatg
     3121 gggggcggaa gttatctgga tcgagaacgt cgcctgggcc gacaccattc gcgttcaacc
     3181 gaactacccg cagctctccc gccgcaattt gcatgcgctg tcgttaaata ttttcaaaga
     3241 tgaagggcgc gaagcgtttc tgaaattaat ggaaaccacc gatatcttca tcgaagccag
     3301 taaaggtccg gcctttgccc gtcgtggcat taccgatgaa gtcctgtggc agcacaaccc
     3361 aaaactggtt atcgctcacc tttccggttt tggtcagtat ggcaccgagg agtacaccaa
     3421 tcttccggcc tataacacta tcgcccaggc cttcagtggt tacctgattc agaacggtga
     3481 tgttgaccag ccaatgcctg ccttcccgta taccgccgat tacttttctg gcctgaccgc
     3541 caccacggcg gcgcttgcag cactgcataa agtgcgtgaa accggcaaag gcgaaagcat
     3601 cgacatcgcc atgtatgaag tgatgctgcg tatgggccag tacttcatga tggattactt
     3661 caacggcggc gaaatgtgcc cgcgcatgac caaaggtaaa gatccctact acgccggttg
     3721 cggtctgtat aaatgcgccg acggctacat cgtgatggaa ctggtgggca ttactcaaat
     3781 tgcagagtgc tttaaagata ttggcctcgc acacctgctc ggtacgccgg aaatcccgga
     3841 aggcactcag cttatccacc gtatcgaatg cccttacggc ccactggtag aagagaaact
     3901 cgatgcctgg ctggcggcac ataccatcgc agaagttaaa gaacgctttg ccgaactgaa
     3961 tatcgcctgt gccaaagtac tgaccgtacc ggaactggaa agcaatccac agtatgtggc
     4021 tcgcgaatca atcactcagt ggcaaacgat ggatggtcgc acctgaaaag ggccgaatat
     4081 catgccgaaa ttcaaaaata accccggaca aatctggcgc ggaatgccct cacatggcat
     4141 ggacacggct gctattttga aaaatatcgg ctacagcgaa aacgacattc aggagttggt
     4201 cagcaaaggt ctggccaaag ttgaggacta aacgcgtggc gtcagtcagc ttgcctgact
     4261 agcgcccgct caataacaaa cgaacaaaat ggatagaggt gcaatggata tcattggcgg
     4321 acaacatcta cgtcaaatgt gggacgatct ggcggacgtt tacggtcata aaacggcgct
     4381 gatttgtgaa tccagcagcg gagtcgttaa ccggtatagt tatcttgagt taaatcagga
     4441 gattaaccgc acggcaaacc tgttttatgt gctggggatt cgcaaaggcg acaaggttgc
     4501 tctacatctc gacaactgcc cggaatttat cttttgctgg ttcgggttgg caaaaattgg
     4561 cgcgattatg gtgccgatta acgcccgcct gttgcgcgaa gaaagcacgt ggatcctgca
     4621 aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt tatcctatgt atcaacagat
     4681 tcagcaggaa gatgcctctc agttgcggca catttgcctg acagatatgg tacttcccgc
     4741 tgatgatggc gtgagttcgt ttactcaacg gaaaaatcaa caacctgcca ccttgtgcta
     4801 tgcaccgccg ctatcgactg acgatacggc ggaaattctc ttcacctccg gcaccacctc
     4861 ccgaccgaaa ggggtggtga taactcatta caacctgcgc ttcgctggat attactccgc
     4921 ctggcagtgt gcactgcgtg acgatgacgt ctacctgacg gtaatgcctg cgtttcatat
     4981 cgattgccag tgtactgcgg cgatggcggc gttttctgcc ggggccacct ttgtgctggt
     5041 cgagaaatac agcgcccgcg ccttctgggg acaggtgcag aagtaccgcg ccaccattac
     5101 cgaatgtatt ccgatgatga ttcgtacgtt gatggtgcag ccgccttcag ccaacgatcg
     5161 gcaacactgc ctgcgggaag tgatgtttta tctcaacctg tcggagcagg aaaaagacgc
     5221 gttttgtgaa cgcttcagcg ttcgcttgct gacgtcttat gggatgacgg aaaccattgt
     5281 gggcattatc ggcgatcgcc ctggcgataa acggcgctgg ccgtcgattg gtcgggcggg
     5341 cttttgctac gaagcggaga tccgcgacga tcacaatcgt cccctcccgg ctggtgagct
     5401 cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc ttcaaagagt attttctcaa
     5461 cccgaaagcc actgcgaaag tgctggaagc cgatggctgg ctacataccg gcgataccgg
     5521 ataccgcgac gaagagggct ttttttattt cgtcgatcgc cgctgcaata tgatcaaacg
     5581 cggtggcgag aatgtctcct gcgtggagct cgaaaatatc atcgccaccc atccaaaaat
     5641 tcaggatatc gtggttgtgg gtattaaaga ttcgattcgc gatgaagcca tcaaagcatt
     5701 tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa ttcttctgct tctgtgaaca
     5761 gaatatggcg aaatttaaag tgccctctta tctggagatc agaaaagatc tgccacgtaa
     5821 ttgctcgggg aaaataatta gaaagaatct gaaataaatg aaacggcagg gaactaccct
     5881 gtcagctaat aatcacgctc taaaacaata tgcatttttt gcagggatgt tgtcgtccct
     5941 gaaaaagcaa aaatggagaa aaggaatgag tgaatcatta catctgaccc gcaatggatc
     6001 aattctggaa attacccttg atcgtccaaa agcgaatgct attgatgcaa aaaccagctt
     6061 tgaaatgggc gaagtatttc taaatttccg tgacgatccg caattacgtg tcgccattat
     6121 taccggtgcc ggagagaaat tcttttccgc gggctgggat ttaaaagcgg cagcagaagg
     6181 cgaagcaccg gatgctgact ttggcccggg aggttttgcg ggattaaccg aaattttcaa
     6241 tctcgacaaa ccggttatcg cagctgtgaa cggctacgcc tttggcggcg gtttcgaact
     6301 ggcgctggcg gcagatttta ttgtttgtgc cgataatgcc agcttcgccc tgccggaagc
     6361 caaactgggc atcgttcctg acagcggcgg cgtgctgcgt ctgccgaaga tcctgccgcc
     6421 tgccatcgtc aatgaaatgg tgatgaccgg cagacgaatg ggcgcagaag aggcgcagcg
     6481 ttgggggata gtcaaccgcg tggttagcca ggcggaactg atggataacg cccgcgaact
     6541 ggcgcagcag ctggttaaca gcgctccact ggcgattgcg gcgctgaaag agatctaccg
     6601 caccaccagc gaaatgccgg tagaagaagc gtatcgctat attcgcagcg gcgtgttgaa
     6661 acactatcca tcggttctgc attcggaaga tgccattgaa gggccgctgg cgtttgccga
     6721 gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga gctattacgc ctttgaggga
     6781 ttaattccgg tggttcaccc gacggcgttt gtccatccca gtgcagtatt gattggcgat
     6841 gtgattgtgg gagccggtgt ctacatcggc ccactcgcct cactgcgtgg tgactacggg
     6901 cggttgatcg tgcaagcggg agccaatatt caggatggct gcattatgca tggctactgc
     6961 gacactgaca ccatcgttgg ggaaaacggc catatcgggc acggagcgat cctgcatggt
     7021 tgtgtgattg gtcgcgatgc actggtcggg atgaacagcg tgattatgga tggcgcgatc
     7081 attggcgaag agagcattgt tgccgctatg agctttgtca aagcgggctt tcgcggcgag
     7141 aaacgccagt tgttgatggg tacgccagcc cgcgctgtac gcagcgtgag tgacgacgag
     7201 ttacactgga aacggttgaa taccaaagag tataaggatc ttgtcgggcg ctgtcatgca
     7261 tcattacatg aaacgcagcc actgaggcaa atggaggaaa atcgcccccg tttgcagggg
     7321 acgacggatg tgacgccgaa acggtgatct ttccggatgc ggcgtaaacg ccttatccga
     7381 cctggctgga aaggaaccgt aggccgtata agatgcgcca gcatcgcatc cggcgaccag
     7441 gtcgcacggt atcaacgaaa cgacattaac gacgcatact ctttgacaac atcatctgcc
     7501 acttctgctc gcggttaagt tccgtgagcg gaacggcagg aatacgtggc gtctttctta
     7561 cgccgactaa tttttcccga ctgttattgc gcaggcgcgc gtaaatttgc tcatcgatat
     7621 cgaccacttt aaccagtcgc tgacactggc atccccgccc ttccagctta ttagggatca
     7681 tcttaacttc gcagcttatt tctgtgactt ccgacagaat ataagtcagg gtattaaccg
     7741 ccttgctgtg ttcaatatcg aaatgaatag agatctctct tgctatcacc caccgatttt
     7801 cagccatcat ccattcggcg attaacaagt agagtggttt ttcaacatat ccttcacaca
     7861 tacgaactcc agtttcacaa attaaaagtc tggctatgac tcgttttgca gcaattaaac
     7921 aaggccatat taaaattgtg gatatatcga tagtatgcaa cttcaccgtc gataagtgtg
     7981 agctaaatcc tgcttatgtc atcaaaaata tcgattcccc aaaagattta cttaatgggc
     8041 aaaaaaaacg gtcctcatca gagaaccgta ttagttattc gatcaaatta gcggatgaaa
     8101 aatatccgcc atgacacgct attatttgat ctgcgcgtgc atttcctgca ccgaaattac
     8161 tttttcagtc gcatcggcat tcagcgccat cgcggtagcg aaaccaccgt tcagggtggt
     8221 gtcgtaatgc actttatatt gcagcgcact gcgacggatc acgcgggagt cttcaatcgc
     8281 acgacgacct gaggtggtgt tgatgatgta ggtatattcg ccattcttga tacggtcctg
     8341 aatgtgcgga cgaccttcat gcaccttgtt caccagacgc ggattaatgc ctgcttcgcc
     8401 cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca gcagttttgc
     8461 cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg gaaagcaacg cacgaccgtg
     8521 tttcttcatg gtggagttgc tgcccagctg cgctttggcg aacgcttcag cgaaggtgcg
     8581 gcccacgccc atgacttccc cggtagagcg catttctggc cctaacagcg ggtcaacgcc
     8641 cgggaattta ttgaacggca gcaccacttc tttcaccgag tagtacggcg ggataacttc
     8701 tttggttacg ccctgctcag ccagcgattt gccagccatc acacgcgccg ccacttttgc
     8761 cagcggtacg ccggtggctt tggagacgaa cggaacggta cgcgccgcac gcgggttaac
     8821 ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc acgttcatca ggccgcgcac
     8881 ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc acatcctgaa tttcctgact
     8941 taaagtgtag gctggtagcg aacatgcaga gtcaccggag tgcacgcccg cctgctcgat
     9001 gtgctccatg atgccgccaa tcagcaccat ttcaccgtcg cagatggcat ccacgtcaac
     9061 ttctactgcg tcatcaagga agtggtccag caacactggc gcatcgttag atacgctgac
     9121 cgctgtctgg aagtagcgac gcaggtcagc ttcgtcatag acgatttcca tcgcccgacc
     9181 gccgagaacg taagacggac gcaccaccag cgggtagcca atctctttcg ctttctcaac
     9241 cgccatttcg atggtggtaa cggtggcgtt cgccggttgt ttcagtttca gacgctcaac
     9301 cgcatgctgg aagcgttcac ggtcttccgc acggtcgatg gcatccgggc tggtaccgat
     9361 aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc agcggggtct gaccgccgta
     9421 ctggacgata acgcctttcg gcttctcaat acgcacaatt tccagcacgt cttccagagt
     9481 taccggctcg aagtagagac ggtcagaggt gtcgtagtcg gtggagacgg tttccgggtt
     9541 acagttaacc ataatggttt cataaccatc ttcgcgcagc gccagcgagg cgtgtacaca
     9601 gcagtagtcg aattcgatac cctgaccgat acggttcggg ccgccgccga gcaccatgat
     9661 tttttcacgg tcggtagacg gattcgcttc gcactcttct tcataagtgg agtacatgta
     9721 agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg cgcttataaa ccgggtgcaa
     9781 gtcatactgg tcgcgcagct tgcggatttc tgcttcacgc acgcccgcga gttttgccag
     9841 acgcgcgtcg gcaaagcctt tgcgtttcag ctggcgcagg aagtcagcgt tcaggccagt
     9901 gatgcccact tctgcgactt tctcttccag acgcaccagc tcttcaattt gtaccaggaa
     9961 ccagcggtca atgttggtca ggttgaacac gccgtctacg gacaggcccg cgcggaaagc
    10021 atcggcgata taccagatac gctctgcgcc tgcatctttc agttcgcggc gaattttggt
    10081 cagcgcttcc ggatcatcga ggctgacttt cgggtcgaag ccagttgcgc ccacttccag
    10141 gccgcgcagc gctttttgca gggattcctg ctgcgtgcga ccaatcgcca tcacttcgcc
    10201 aaccgatttc atctgagtgg tcagacggtc gttcgccccg gcgaatttct cgaagttgaa
    10261 gcgaggaatt ttagtcacca catagtcgat ggacggctcg aaggaggccg gagtacgccc
    10321 accggtgatg tcgttcatca gctcgtcgag ggtgtaaccc accgccagtt tcgccgccac
    10381 tttggcaatc gggaaaccag tggctttcga cgccagcgca gaggagcgag acacgcgcgg
    10441 gttcatttcg ataacaatca ggcgaccgtt tttcgggttc accgcaaact ggacgttgga
    10501 accaccggtt tcaacgccga tttcacgcag taccgccatc gaggcgttac gcatgatttg
    10561 atattctttg tcggtcagcg tttgggctgg cgcgacagtg atggagtcac cggtgtggat
    10621 gcccatcgca tcaaagtttt cgatagagca gacaatgatg cagttgtcgt ttttatcacg
    10681 caccacttcc atctcgtact ctttccagcc gatcagcgac tcatcaatca gcagctcttt
    10741 agtcggagag agatccagac cacgggcgca aatttcttca aactcttcgc ggttataagc
    10801 gataccgccg ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg ggaagccaac
    10861 ttcagcggca accgccagcg cttcttccat tgaatgagcg ataccggaac gcgcggtttc
    10921 cagaccgatt ttcttcatcg ctacgtcgaa acggcggcgg tcttcggctt tatcaatcgc
    10981 atcggcagtg gcaccaatca tggtgacgcc gaactcttcc agcacgccct gacgctcaag
    11041 ctcaagcgca cagttcagcg ccgtctgccc ccccatggtt ggcagcaccg cgtccggacg
    11101 ctctttttca ataatcttgc gtaccacttc ccagtgaatc ggttcgatgt aggttgcatc
    11161 agccatttcc gggtcggtca tgatggtcgc cgggttggag ttcaccagaa tgacgcggta
    11221 accctcttca cgcagggctt tacacgcttg cgcgccagag tagtcaaact cacacgcctg
    11281 accgataaca atcgggcccg cacccagaat caggatactt tttatatctg tacgttttgg
    11341 catggctctt ttactcctga ttacttagcg gttttacggt actgctcaat taactcgata
    11401 aagtggtcaa acaacggcgc ggcgtcgtgt ggaccagggc tggcttccgg gtgtccctgg
    11461 aagctgaacg ccggtttatc ggtacgatga atgccctgta acgtaccgtc gaacagggat
    11521 ttatgtgtga cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa accgtggttc
    11581 tgggcggtaa tcatcaccac atttttctca acatctttaa ccggatggtt gccgccgtgg
    11641 tgaccaaatt tcatttttac agtcttcgca ccgctcgcca gcgccagcag ctgatgaccg
    11701 aggcagatgc cgaataccgg aatatcggtt tcgaggaatt tctggatggc ggtaatggcg
    11761 taatcgcacg gggccgggtc gccaggaccg ttggagagga agatgccgtc tggattcatt
    11821 ttcagcacgt cttccgcaga agtttgcgcc ggaacgatgg tcagacgaca gcctctatcc
    11881 accaacatgc gcaggatgtt gcgcttggca ccaaaatcat aagccacaac atggaacggc
    11941 agctcgtctt ctttcttcgc ttctggcagg ccgccggtca acgtccagct cccttgtgtc
    12001 cagctatagg cttctgcggt ggtcacttct tttgccagat ccatgccgtt cagacctggg
    12061 aacgcgcggg ctttttctaa tgccagcgcc gcatccgggt tatcgcctgc gataatgcag
    12121 ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc gatatcggca
    12181 atcgccacga tgttatggcg cttcaggtaa gaagagaggt cttcggtatt acggaagttg
    12241 ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg catgtacctg agaagattct
    12301 tcatcggcgt cattagtgcc gacattacca atatggggat aagtaagagt aacgatttga
    12361 cgagaatagg aaggatcagt gaggatttct tgataaccgg tcattgaagt attgaaaacg
    12421 acttccccaa ccgccgaacc tgttgcccct atggcccgac cgtgaaactg ggttccgtct
    12481 tccagaacca atagcgctga cttaatcaaa acaccctcca gagaatattc actcacttta
    12541 tttgcatatt aattcacaat gatgacgtaa atcaatgcaa atctgcttac cgacgaattt
    12601 ctggcaaacg gcggcattct ggagatatgg gcgctaaaag tcaacttaat ggcgggattt
    12661 tttatattat tttgtggctc atgcatgttt tacaggcatt aagcgacaaa aagatcatat
    12721 aacatgttct ggaaaacgct tgcgcagcaa gaagattgca tgaaatcaaa atgagcagaa
    12781 aaaaagtgga ccaaatggtc aaaatttaca ttaaaacaac aaaaacagaa tacaaaacat
    12841 gtaattttca gcacttgcat taaatttaaa agcataacac caaaaataaa agggctaaaa
    12901 attaaagccc tttatattca atgcattaaa tgtgttattt ttgcatcact ataaatattt
    12961 tgtggttaca gattattgag atcaagcaca tctcgcatat caaaaagacc actttcctta
    13021 ccactcaacc acaaagccga tcttaccgca ccgttagcaa atgtcatgcg gctggacgcc
    13081 ttatgggtga tctccagacg ctcgccaata tcggcaaaca tcgcggtatg ttcaccaacg
    13141 atgtcacctg cacgcacggt ggcaaaacca atggtgccag gcacacgttc accggtatgg
    13201 ccttcacgac tgtagaccgc gcaatctttc agatctttat ccagagcgtg ggcgatcgcc
    13261 tctcccattg ccagtgcggt gcctgacggc gcatcaactt tatgtctatg atgtgcttca
    13321 ataatttcga tatcggtgta gtcacccatc actttggctg ctttctccag cagtttaagc
    13381 atgacgttaa cgccaacgct aaaattggcg gcaaagacaa tcgcaatatc ggcggcggcg
    13441 tcacgaatcg cctgtttacc ggcttcgtca aaccccgtag tgccgatcac catccctttg
    13501 ccatgctggc gacaaaaagc gagatggttc agcgtacctt ccggacgggt aaaatcgata
    13561 aacacatcaa aatcatcttt tatcgcatca agactacttt gcacagtaac gcctgttttc
    13621 ccggctccgg ccagttcgcc cgcatcgctg cccagtaaag aagatccttc gcgctccagc
    13681 gccgcgccca gctgcacgcc ttctaatgcc agcgccgcct gaatcaactg gcggcccata
    13741 cggcccccgg ctcccgcgat ggcaacgcgg atgtttgcat catgcatagc tattctcttt
    13801 tgttaatttg catagaccgt tttcagagta accagcgcat cgctacgccg ccagccaaaa
    13861 acgctgataa ttagaaatta atgataaaca gggagggata tcagtgaaag gcatgagtcg
    13921 atgaatgact gcatgccgat aacatgtgac aggttacggc accagagcca gcacctcagc
    13981 tacccactgc tggaagcctt ttacattcag atccaatgcc acctgtacat tggctggttt
    14041 gcccaggcaa ccgtcgatat caaccaccgt cgtgccagag gtaaattcgc cctgcgtttc
    14101 cactgccaca aaacacggct tgagagtgaa caggtccggg cgcaccagcc aggcgatggc
    14161 gcagagatcg tgcattcgca gtccactttg catactgccg ctacggtagt gactaaacag
    14221 ggcgtggagc atttttccgg tgcgattcaa ctccggcaag gtagcgagat agtcaggggt
    14281 taatattgcc tgattggtga catccagacc gcacatgacg atttcgatgc cgctacggaa
    14341 gacacaggct gccgcttctg gatcggcagc gatattaaac tcggcgtttg gtgtgcagtt
    14401 gccgcgacca gcagaaccgc ccatgatcac cagacggcga atatacggct tacattccgg
    14461 gcattgtgaa agtaacagcg caatattggt taacgggccg atggccacca gggtaacggg
    14521 ctctggcgca cgcatcaggg catcacgaat tgccagaaac gccggtatac ccagtggctg
    14581 acggttgtgt tcaacaaagt catagccagc cattcccgat tcaccgtgta cagatgccgc
    14641 atcgcgcggc gcacgaacca gtggtacggc agcgccctgg gcaagcggaa tctccgcatt
    14701 ccagaaatgc agcagttgca gggcgttgcg ggtcgttttc tctaccgaaa cattacccgc
    14761 gacagtggtc atcagttgca gatcgagttc gggggtaaaa atcgcggcgg caatggcaac
    14821 ggcatcgtca atgccggggt ctgtatcgag gaagataggt aaacgcatgt tttctccata
    14881 aaaaatgccg gtaacaagac cggcattttc gcataactta ggctgctaat gacttaatcg
    14941 acttcacgaa tatcgacacg cagctctttc ggcacttcga aaacaatgtt ttcttcacga
    15001 ccttccagcg gaatggcttc accaccgccc agctgttgca aacgtgccac cacattctgc
    15061 accagaatat ccggagccga tgcgcccgca gtcacgccaa cgcagttcac gccttttacc
    15121 cactcttcct ggatatcttt cgcatcgtca atcaaaaatg cgcgtttgcc catacgctgg
    15181 gccagttccg ccagacggtt ggagttggag gagtttttcg aaccaaccac cagtactact
    15241 tccgcctgct ctgccagggc gcgtaccgcc tcctgacggt tagtcgtggc gtagcagatg
    15301 tcatctttgc gcggaccgac aattttcggg aagcgtttac gcagcgcgtc gatcacatca
    15361 gacgtgtcat ccaccgacag cgtggtttgg gtcataaagg agagcttctc ttcgtttttg
    15421 accgtcagtt tccacacatc gtctggcgat tcgaccagat acattccccc ttccgggttg
    15481 ctgtactggc ccatcgtccc ttccacttcc gggtgcccgg cgtgaccgat gagaatagat
    15541 tcttcaccac gacggctggc gcgggcgact tccatatgca ctttggtcac cagcggacag
    15601 gtggcatcaa acaccgtcaa atcgcggctt ttcgcttcgt tacgtactgc ctgagaaaca
    15661 ccgtgtgcgg agaaaatcag gatcgcgccg tccggtactt cgctaatctg ctcaataaag
    15721 atagccccac gctcgcgcaa gctatcgacc acgtagcggt tatgcaccac ttcgtgacgg
    15781 acatatatcg gtgcgccgta aatggccagc gcgttttcaa caatgctgat agcgcggtct
    15841 accccggcac aaaaaccacg cgggttggcc aacaggatct gcatgttacg cctccagtgc
    15901 cggatcgatt tccagcactt caatatcaaa atgaacggtc tgcccggcca gcggatggtt
    15961 gaaatcaacg gtaatggagt cgccgttaat ttcgcggatc acgccaggca tctcactgcc
    16021 atccattgcg gtaaaaagca tgattgcgcc aatttctggc tcgcctgcat ccataaattc
    16081 acggcgggag aagtactgaa tcaggtccgg tgacggcacg ccaaacgccg catcaggctc
    16141 cagcgagaag gtggttttat cgcccacttt cagccccagc aggtgttgct ccagcccttc
    16201 agaaagagaa gcatcaccca ggcggaacag cgccggttta ccgttgttgc gggtagactc
    16261 agcggtggtg ccatcatcga gtttcagcgt gaagtgcacc aggacggcgc tattgctctg
    16321 tacagattca gacatgcagg tttgctctta tattttgagg atttttgccg gatgcggcct
    16381 acgatttcgc agcaatctgt aggccggata agatgcgtca gcatcgcatc cggcagggtt
    16441 tattattgtt ttttcgcttt agaaggtaaa aaaccttcca gcacaatcag tgccgcaccg
    16501 acacaaatgg cagtatcggc aaggttgaag gtggcgaagt gccagtcgcc gacatagaag
    16561 tcgatcatat cgacaacgaa gccgtgccac aggcggtcga acaggttgcc cagcgcgccg
    16621 ccaataatca gcgcgtaagc gatattgttt agcttctgcg tggccttcga gcgatacatc
    16681 atcactgcca ggatcacgct aataccaatc gcaataccgg caaaaaacca acgctgccag
    16741 ccgccactat cggcaaggaa actaaacgcc gcgccatagt tacgcgcata atgcagatta
    16801 agcgacggga acagcgggac cgtatccccc agagcaaagt tctggaggat caggtatttg
    16861 ctgcccagat cgataatcag cacgactacc accagccaca gccagcgtag ccctgttgaa
    16921 cagatcgatt gactcatcag gcaaacttac gtttttcacc gtcaccggcg acgttgctga
    16981 cacagcggcc gcagatttct gcgtgttccg ccaccttgcc gacatcctgg gtgtagtgcc
    17041 agcagcgtgg gcacttctca ccttcggctt tactcaacgc gactttcagc cctttgagga
    17101 cttcgctctg ctgggcgtca gcaggtgcgt cgttatagtc tgcaacggta gcgccggagg
    17161 tcaacaggac aaatcgtaat tcatcgccca gcgcggtcag tttcgccgcc agttccggtt
    17221 ctgcatacaa ggttactgcc gcttccagcg agccgcccac tttcttatcg gcacgcgctt
    17281 gctcaatgac tttgttcact tcgccacgca ctttcaacag ctcgtcccag aacgcatcgt
    17341 tcattgcttc actgtctgcc agaccaaaca ggccttcgta ccactcaccg gtgaagacgt
    17401 atttttcacg ttcgcccggc aggtaacccc acacttcatc agcggtgaag gagaggattg
    17461 gtgccatcca gcgaaccagc gcttctgcga tgtgatacag cgcagtctgg cagctacgac
    17521 gcgccacgct gtccgctttg gcggtatact gacggtcttt gatgatgtcg aggtagaagg
    17581 accccatctc aacggagcag aagcgcatca gacgctgtac cacttcgtgg aaatcgtatg
    17641 cttcgtacgc tttgaggatg tcttcctgtg ccgcgttcgc acaacctacg gcccagcgat
    17701 ccagtaccac catctcttcc ggtttcacca tatcttttgc cggatcaaaa ccgttcaggt
    17761 ttgccagcag gaagcgcgcg gtgttacgga tacgacgata gctgtcggca gcacgtttca
    17821 ggatctcgtc agaaacggcc atttcgccag tgtagtcggt tgatgccacc cacagacgca
    17881 gaatatccgc acccagtttg ttcatcacat cctgcggcga aacggtgtta ccgatggatt
    17941 tagacatctt gcgaccctga ccatccacgg taaagccgtg agtcagtacc tgacgatatg
    18001 gcgctttgcc cttcatcgcg gtagagatca tcagggaaga catgaaccag ccacggtgtt
    18061 ggtcagaacc ttccagatac atgtccgctg cgtgaccggc aaattccgga cgcacatcaa
    18121 caacggaaga gtgggtagat ccggagtcaa accatacatc cagcgtatcc ggtactttca
    18181 catactggtc agcttcgtcg ccgaggatct ctttcgcatc gagatcccac cacgcctgaa
    18241 tgccgtcaac ttcaacgcgt tttgccactt cttccatcag ttcgagagta cgcggatgca
    18301 gttcttctgt gtctttgtgc acgaacagtg acatcggcac gccccaggta cgctgacgag
    18361 agatacacca gtcaggacgg ttagcaacca tcgactcgat acgcgcctgg ccccagtcag
    18421 ggatccactg cacgcctttg atctctttca gtgactgcgc acgcagacct ttctgatcca
    18481 tgctgacgaa ccactgcggc gtcgcgcgga agatgatcgg cgttttatga cgccagcagc
    18541 acggatagct gtgttgcatt ttctcaacgt gcaacagtgc gcctttttcc tgcaacaacg
    18601 caatgacaat atcgttcgct ttgaagacgt taacgccatc cagagtcggg taagtacccg
    18661 gcagataagt gccgtccggg ccaaccgggt tagcggtttc cagaccatat ttttgaccga
    18721 tcacatagtc gtccggaccg tggcctggcg cggtatgaac ggcaccggta ccggcatcca
    18781 gcgtaacgtg gtcgccgaga attgccggaa catcgaagtc cataaacgga tgggtaaagc
    18841 gcaacagttc cagctcggca cctttcaccg tgccaagaat ggtgtaatcg ctaacgccga
    18901 tacgctgcat tacgctttca accagatctt tcgcgaggat cacggcctga ccgtcgattt
    18961 gcaccagcgc ataatcaaaa tcaggtgcga tggagattgc gcggttagca ggcagcgtcc
    19021 acggcgtggt ggtccatata accagcgaaa ttgggccgtt aacattgctt acgccaaatt
    19081 tcgttttaag cgcatcctga tcgaccgcct ggaaagcgac gtcgatggac agagaagttt
    19141 tgtcgtaata ctcaacttcc gcttctgcca gtgcagaacg gcagtcaacg caccagtgca
    19201 ccggcttcgc gcctttgtgc aggtgaccgt tgccgatgat tttgcccagc gcgcggatga
    19261 tgttggcttc agttttgaag tccatggtca ggtacgggtg cgaccagtcg cccagcacgc
    19321 ccagacggat aaagtctttg cgctgaccgt caacctgggt cgcagcgtat tcgcggcact
    19381 tggcgcggaa ctcagcggcg gtgaatttct cccccggctt accgtattct tgctctactt
    19441 tcagctcgat tggcagacca tgacagtccc agccaggcac atacggcgag tcatatccag
    19501 aaagcccttt ggacttaatg ataatgtctt tcagaatctt gttaaccgag tgaccaatat
    19561 gaatactgcc attcgcataa ggagggccat catgcagaat gaaggttttt ttgccttttt
    19621 tagctgcacg aatgatgccg tacagatcat catcagtcca acgcgccagc attcccggtt
    19681 cgcgcttggc gagatcgcca cgcatcggga accctgtttc cggcaaattc agggttgatt
    19741 tatagtcact catcagattc tcggttccgt atttcggttt gattacataa caggcttaag
    19801 ccggttttgt tagcccaaaa aattcgcggg cggttaattc atcacgcgca atctgcgctt
    19861 tcagttcgtc cagcgatgca aatcgctgct cattgcgtat ttttttacgc agcactactt
    19921 gtatatggcg accataaagg tccattgcaa catctaacaa atgcacttcc agttgctggc
    19981 gaataccggc aaccgttggg cgtgttccga tgtttgcaac gccgggtaac ggcttttcgc
    20041 caaggcccaa cacttctacc gcataaaccc ctttcaccgg ggaaacctga cggcgtagcg
    20101 gtacattcgc cgtcgggaaa cctatagtgc gccctaattc atcaccgtgg actacacgcc
    20161 cggagatagc aaacgggtgc cccagtaaac tttctgccag agccagattg tcatccgcaa
    20221 gcgcctgacg cacggcggtg ctgctgatac gcacgccacc ttcgcaaaaa gtttgcgtgc
    20281 tggtgatatc gaagccgtat tccatgcccg ctttctgtaa taacaagaaa tcgccttcac
    20341 gaccagcgcc aaagcggaaa tcgtcaccta cggcaagaaa ttttacccgc aagtgcttca
    20401 ccagaagatc actgatgaag ttttgcgcgg ttaacgccgc aaaacgcctg tcaaaacgca
    20461 cgcacagcac gtaatcaaca ccacactctg caagataacg cagtttttcc cgcagccggg
    20521 tgagccgtgc cggggcttta tcagtagcaa acagttccag tggttgaggt tcaaaaagca
    20581 tcaccatcac cggtaagttg cgcttgcgcc cttcttcctg caagccctgt aacagcgcgc
    20641 gatgaccgcg atgcacgccg tcgaaattac caatagtcag cacacaccct tcttgcgggg
    20701 cctggctgag attatgtatg ccgcgtatca gcttcatgtc tggctcaaaa cagtgaaaat
    20761 cgtccgagta taccttgtac agcggtcaag gttaaccggc gattgagtac cgaaatcaga
    20821 aagaagtggc ttttcattgc tatggcgcaa atcatgggaa gaaactgacc gcctgctgca
    20881 atttttctcg cggaaaagct gtattcacac cccgcaagct ggtagaatcc tgcgccatca
    20941 ctacgtaacg agtgccggca cattaacggc gcttatttgc acaaatccat tgacaaaaga
    21001 aggctaaaag ggcatattcc tcggcctttg aattgtccat atagaacaca tttgggagtt
    21061 ggaccttggc taatatcaaa tcagctaaga agcgcgccat tcagtctgaa aaggctcgta
    21121 agcacaacgc aagccgtcgc tctatgatgc gtactttcat caagaaagta tacgcagcta
    21181 tcgaagctgg cgacaaagct gctgcacaga aagcatttaa cgaaatgcaa ccaatcgtgg
    21241 accgtcaggc tgctaaaggt ctgatccaca aaaacaaagc tgcacgtcat aaggctaacc
    21301 tgactgcaca gatcaacaaa ctggcttaat tgcctgttgt tgtcagcttt gtaaaaaaac
    21361 ccgctctggc gggttttttt atatccatct gcaggccaaa attcggcctg caaagtacaa
    21421 atgtttattt tgaagctgga gtaaacagcg cagaatagtc tgtattgcag atacgctgta
    21481 ctgcagggtg ctgaatcatc ctttcggcaa aaatcgcgtg gtactcttcc atcacgttct
    21541 caacacggcc tatctccaca accgagtcat cgttatagaa atcattagcg taaagcgaag
    21601 gtgcaacgaa aatagcgtta tgcgtcgccc caaaggcttt catcaacgca gcatcatcaa
    21661 actcacccaa aatttcgacg ttcaagccct gggagttaaa ccagtttaat agcttacgcc
    21721 ccaacattga gcgacgcccc ggaataagta aacgacgctc ttcaagacag gcaggaaacg
    21781 gcttttctgg tagtgggtta gtgcaccaga aactgacacc acattcgcca attttcatgg
    21841 aaaacagccc ttcctgctga gtggaatcga tcggacagtc agagatgatc atatccagtt
    21901 tatgctgact caactgctct aaaagcatct cgtgcgtcga ttcgaaacag cgtagatgga
    21961 tctgctcgtc ttccacaact gcggcatcca gaacactgct gaccagacgt ttggaaagtg
    22021 catctgccac accaacatca aacaataagt tggactcttt gcgatagttg acgatatcca
    22081 gcatttcctg gcttaaggtg aacattttat cggcatagcg atagaccagc tctcccagct
    22141 cgctgggctc gaggccacgt cccttgcgct taaataattt gccttgcagg cgctcttcca
    22201 gcgcccgaat ctgtccggta atggtttgtg gtgttaaata aagcgcctcc gctgcgccaa
    22261 ccacggaacc ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag
    22321 acatgctcat ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa
    22381 accgtcctgt caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc
    22441 cgaagagata gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac
    22501 gctaccaaag gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat
    22561 ccccaccgcc ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg
    22621 cagcgccaac cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat
    22681 gatccccaat ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc
    22741 agcattagca aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaacacatg
    22801 ctccagtcgc ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac
    22861 aattaccccc gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac
    22921 gccaaccaga atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac
    22981 cgcaattgct acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa
    23041 tgcgatgata atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt
    23101 cagggctaac ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aagcaatgtc
    23161 agtagccgcc gggatcgccc acccttcgcg ggtaatcgga tcggcatggt taaaggccag
    23221 atagagtaat gccggcacaa tcatcccacc aatagcggcg ataactggaa atgcagcctg
    23281 gcgtaagctg gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag
    23341 gaaaaatacc gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag
    23401 tgaaccaacc cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc
    23461 gccgctgttg gccataatca tcgccaggat agcggcaatg ataagaataa tgcctcccga
    23521 ggcatcactg ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat
    23581 agatcgtttt tactatctta ccccgcacga atcatcgtta aaatcagatt atagtgtagg
    23641 aataattcgc tttttacgag gcattgaagg atttgcgtca tcagtgatga gatgagtttt
    23701 tgacggctgt aagcgctgac gtgtgaccct aagccctga
//
LOCUS       DAZGAP010000077        23000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGAP010000077 DAZGAP010000000
VERSION     DAZGAP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.077"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..528)
                     /gene="fliZ"
                     /locus_tag="ACHLAM_002536"
     CDS             complement(<1..528)
                     /gene="fliZ"
                     /locus_tag="ACHLAM_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO7854046.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSS"
     gene            complement(574..1293)
                     /gene="fliA"
                     /locus_tag="ACHLAM_002537"
     CDS             complement(574..1293)
                     /gene="fliA"
                     /locus_tag="ACHLAM_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO7854047.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1612..3351)
                     /locus_tag="ACHLAM_002538"
     CDS             complement(1612..3351)
                     /locus_tag="ACHLAM_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO7854048.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVSAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLDGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQYTGTVFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
     gene            3617..5023
                     /gene="fliD"
                     /locus_tag="ACHLAM_002539"
     CDS             3617..5023
                     /gene="fliD"
                     /locus_tag="ACHLAM_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO7854049.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLIIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            5048..5458
                     /gene="fliS"
                     /locus_tag="ACHLAM_002540"
     CDS             5048..5458
                     /gene="fliS"
                     /locus_tag="ACHLAM_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO7854050.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            5458..5823
                     /gene="fliT"
                     /locus_tag="ACHLAM_002541"
     CDS             5458..5823
                     /gene="fliT"
                     /locus_tag="ACHLAM_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO7854051.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            5901..7388
                     /gene="amyA"
                     /locus_tag="ACHLAM_002542"
     CDS             5901..7388
                     /gene="amyA"
                     /locus_tag="ACHLAM_002542"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7854052.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(7422..7835)
                     /gene="yedD"
                     /locus_tag="ACHLAM_002543"
     CDS             complement(7422..7835)
                     /gene="yedD"
                     /locus_tag="ACHLAM_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO7854053.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     SALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            8022..9227
                     /gene="yedE"
                     /locus_tag="ACHLAM_002544"
     CDS             8022..9227
                     /gene="yedE"
                     /locus_tag="ACHLAM_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO7854054.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            9224..9457
                     /gene="yedF"
                     /locus_tag="ACHLAM_002545"
     CDS             9224..9457
                     /gene="yedF"
                     /locus_tag="ACHLAM_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO7854055.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            9566..10234
                     /gene="yedK"
                     /locus_tag="ACHLAM_002546"
     CDS             9566..10234
                     /gene="yedK"
                     /locus_tag="ACHLAM_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO7854056.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAVREWMRQEVGGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAAL
                     IQPV"
     gene            10345..10824
                     /gene="yedL"
                     /locus_tag="ACHLAM_002547"
     CDS             10345..10824
                     /gene="yedL"
                     /locus_tag="ACHLAM_002547"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7854057.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PKVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
     gene            11064..11204
                     /locus_tag="ACHLAM_002548"
                     /pseudo
     CDS             11064..11204
                     /locus_tag="ACHLAM_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(11229..11543)
                     /gene="fliE"
                     /locus_tag="ACHLAM_002549"
     CDS             complement(11229..11543)
                     /gene="fliE"
                     /locus_tag="ACHLAM_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO7854058.1"
                     /translation="MSAIQGIEGVISQLQATAMSARTQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            11758..13416
                     /gene="fliF"
                     /locus_tag="ACHLAM_002550"
     CDS             11758..13416
                     /gene="fliF"
                     /locus_tag="ACHLAM_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO7854059.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            13409..14404
                     /gene="fliG"
                     /locus_tag="ACHLAM_002551"
     CDS             13409..14404
                     /gene="fliG"
                     /locus_tag="ACHLAM_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO7854060.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            14397..15083
                     /gene="fliH"
                     /locus_tag="ACHLAM_002552"
     CDS             14397..15083
                     /gene="fliH"
                     /locus_tag="ACHLAM_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO7854061.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGVAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            15083..16456
                     /gene="fliI"
                     /locus_tag="ACHLAM_002553"
     CDS             15083..16456
                     /gene="fliI"
                     /locus_tag="ACHLAM_002553"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO7854062.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGSALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            16475..16918
                     /gene="fliJ"
                     /locus_tag="ACHLAM_002554"
     CDS             16475..16918
                     /gene="fliJ"
                     /locus_tag="ACHLAM_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO7854063.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            16915..18042
                     /gene="fliK"
                     /locus_tag="ACHLAM_002555"
     CDS             16915..18042
                     /gene="fliK"
                     /locus_tag="ACHLAM_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO7854064.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDKQSTSTSLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            18147..18611
                     /gene="fliL"
                     /locus_tag="ACHLAM_002556"
     CDS             18147..18611
                     /gene="fliL"
                     /locus_tag="ACHLAM_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO7854065.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            18616..19620
                     /gene="fliM"
                     /locus_tag="ACHLAM_002557"
     CDS             18616..19620
                     /gene="fliM"
                     /locus_tag="ACHLAM_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO7854066.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            19617..20030
                     /gene="fliN"
                     /locus_tag="ACHLAM_002558"
     CDS             19617..20030
                     /gene="fliN"
                     /locus_tag="ACHLAM_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO7854067.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            20033..20398
                     /gene="fliO"
                     /locus_tag="ACHLAM_002559"
     CDS             20033..20398
                     /gene="fliO"
                     /locus_tag="ACHLAM_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO7854068.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            20398..21135
                     /gene="fliP"
                     /locus_tag="ACHLAM_002560"
     CDS             20398..21135
                     /gene="fliP"
                     /locus_tag="ACHLAM_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO7854069.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLTGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            21145..21414
                     /gene="fliQ"
                     /locus_tag="ACHLAM_002561"
     CDS             21145..21414
                     /gene="fliQ"
                     /locus_tag="ACHLAM_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO7854070.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            21423..22208
                     /gene="fliR"
                     /locus_tag="ACHLAM_002562"
     CDS             21423..22208
                     /gene="fliR"
                     /locus_tag="ACHLAM_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO7854071.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            22498..>23000
                     /gene="rcsA"
                     /locus_tag="ACHLAM_002563"
     CDS             22498..>23000
                     /gene="rcsA"
                     /locus_tag="ACHLAM_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO7854072.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKT"
BASE COUNT         5615 a         5873 c         5983 g         5529 t
ORIGIN      
        1 actggaggat ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt gatacttccg
       61 taacgcaatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga ggatttcatc
      121 aaggaaaccg tcctgcagct gatcgaggga aatattttgc tcgtgtagat gattacccaa
      181 acggcgcaga cgaacgacat attcacgcac cgtacctggg ctcatttcag tttgctcaaa
      241 aagaaattgc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc
      301 accgcaaaat cggcacaatg ccgcccatga tttttgttcc acttgccagc cattttcatc
      361 aagcagcatg tccaggcggg aaatctcgat tttattcact attttgccgt cgcgaacgag
      421 ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc
      481 tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta agaactcctg
      541 gtagtcaaaa ttaaagtgcg gcatttactg acgttataac ttacccagtt tagtgcgtaa
      601 ccgtttaatg gcctggctgt gtaactgact gacccgcgat tccccgacct ccagcaccgc
      661 gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca gtttttcgcg
      721 ctccggcaac gtttcgatag cttccatcac ccgctggcgc agattactgt ccagcagttg
      781 ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat cgccgtgctc
      841 ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca acatttggcg
      901 atagtcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc
      961 aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct
     1021 gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga tacgctgcac
     1081 tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg cattgagtaa
     1141 cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg
     1201 caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg agtgtttatc
     1261 cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt tatatgagtt
     1321 atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag
     1381 gggttatttg ggggttaccg gtaaattgcg ggcagaaaaa accccgccgt tggcggggaa
     1441 gcacgttgct gacaaattac cattcatgtt gccggatgcg gcgtaaacgc cttatccggc
     1501 ctacaaaatc cgcaaattca ataaattgca attcaccttg taggcctgat aagcgcagcg
     1561 catcaggcaa tttggcgttg ccgtcagtct cagttaatca ggttacgacg attaaccctg
     1621 cagcagagac agaacctgct gcggtacctg gttagctttt gccagcacgg agttaccggc
     1681 ctgctggatg atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg
     1741 aatacgggac tgcgcttcgg acaggttggt agtggtgttg ttcaggttgg tgaccgcgga
     1801 atccagacgg ttttgcaccg caccgaggga ggagcggaat ttgtcgatgg agctgatggc
     1861 ttcgtccaga gctttcagtg gatcggcggt ggttgcagct ttagtttcag cgtcagtagt
     1921 aaaattacca tccgctgaaa catagactgc tttacctgaa ccattggtca cagttccgtc
     1981 tttttgcaaa aacagattgg ttgtattatc ggtagtcagt gaaccatcag ctttggtgta
     2041 agcgtcttca aaaactgtac ctgtgtattg ttgtactata cctttactat caactttaat
     2101 atcgcctgcg ctggtaccag tagctccaac atcaactgtg aatcctgtgg acttagttgc
     2161 cgattgaaga gactcagcac tcattcttgc atttgctaca gtgattgcac cagaagtagt
     2221 agcatcagta ccgccatcca gagtgaaggt tgtttcatct tcagttgtaa ttaccgtaga
     2281 tacggcaccc ggtgtgcctg ttgtatgcca gtttttagtc agtgcatcaa gagtggctgc
     2341 tttgacagtc ccagcattgt tttgagtgag gttaccatct acgtcaataa atagttctga
     2401 accatctttc gcggtaattt taccgtcgct agccaataca acgtcaattg acgtagaacc
     2461 aattttaacg tttaagttag cagtatcgcc cgcttttggt gtcaggaagg attgtaactg
     2521 agcactgtta ctatcaccag aatttgtacc agctgcaata gttgcactat atgtaaaatc
     2581 acctgtagcg ctgttgtatg tataatctgt agctgttggc gatccaaaac catttgcaat
     2641 tgtggcatta acttttgcgt tattagccaa actcgcaatt acatctgcag ctgtagatgt
     2701 tttcaggcct gcgctaacag tataggtaac tgtaccatta gcatcagcag tacctggagc
     2761 caagagttga gctgctgcca aatcagattt agtcgctgca gtattagcca cagccccgcc
     2821 accattcaca ttaaacccac tcagccccag cgtatcagag tcaattttct tcagatcaat
     2881 agtgatagtc tggccgtcat tcgcaccaac ctgaattttc attgaaccgt ctttcgccag
     2941 tacgttcacg ccgttgaact gggtctggcc agatacgcgg tcaatttcgt ccagacggga
     3001 tttgatttcg tcctgaatgg agtccagatc cgaatcggag ttagtcccgg tagaagcctg
     3061 aaccgtcagt tcacggatac gctgtaagtt gttgttgatt tcggacagcg cgccttcggt
     3121 ggtctgtgca acggagatac cgtcgttggc gttacgtgca gcttgagtca gacctttaat
     3181 gttagaagta aaacggttag caatcgcctg acctgcggcg tcatccttcg cgctgttaat
     3241 acgcaagcca gaagacagac gctcgataga actcgacagc gcagactggt tcttgttgat
     3301 attattttga gtgatcagcg agaggctgtt ggtattaatg acttgtgcca tgattcgtta
     3361 tcctatattg caagtcgttg attacgtatt gggtttccac ccgtcggctc aatcgccgtc
     3421 aaccctgtta tcgtctgtcg taaaacaacc tttagaattt ttttcacaaa cagccatttt
     3481 ttgttagtcg ccgaaatact cttttctctg ccccttattc ccgctattaa aaaaaacaat
     3541 taaacgtaaa ctttgcgcaa ttcaggccga taaccccggt attcgtttta cgtgtcgaaa
     3601 gataaaagga aatcgcatgg caagtatttc atcgctggga gtcgggtcag gtctggattt
     3661 aagttcaatc cttgatagcc tcaccgccgc gcaaaaagcg acgctaaccc ctatttcaaa
     3721 tcagcaatcg tcgtttaccg ctaaacttag cgcctacggt acgctgaaaa gcgcgctgac
     3781 gactttccag accgccaata ctgcattgtc taaagccgat cttttttccg ccaccagcac
     3841 taccagcagc accaccgcgt tcagtgccac cactgcgggt aacgccatcg ccgggaaata
     3901 caccatcagc gtcacccatc tggcgcaggc gcaaaccctg accacgcgca ccaccagaga
     3961 cgatacgaaa acggcgatcg ccaccagcga cagtaaactc atcattcaac aaggcggcga
     4021 caaagatccg attaccattg atatcagcgc ggctaactca tcgttaagcg ggatccgtga
     4081 tgctatcaac aacgcaaaag cgggcgtaag cgcaagcatc attaacgtgg gtaacggtga
     4141 atatcgtctg tcagtcacat caaatgacac cggccttgat aatgcgatga cactctcggt
     4201 cagcggtgat gatgcgctac aaagttttat gggctatgac gccagtgcca gcagcaacgg
     4261 catggaggtc tcggtcgccg cccagaatgc gcagctgacg gtcaacaacg tcgccattga
     4321 gaacagcagc aacaccatca gcgacgcgct ggaaaacatc accctgaacc tgaacgatgt
     4381 caccacgggc aaccagacgc taaccatcac tcaggacacc tccaaagcgc aaacggcgat
     4441 taaagactgg gtgaatgcct ataactcgct aatagatacc ttcagcagcc tgaccaaata
     4501 caccgccgta gatgcgggag ctgatagcca gagttctagc aatggcgcac tgctcggcga
     4561 ctccacgttg cggacgattc agacgcagtt gaaatcgatg ctgagtaata ccgttagttc
     4621 ttccaactat aaaacgttgg cgcagattgg tatcacgacc gatcccagcg atggcaaact
     4681 ggaactggat gccgacaaac tcaccgctgc actgaaaaaa gatgccagcg gcgtaggtgc
     4741 attgattgtt ggcgatggta aaaaaaccgg catcacgacc accatcggca gcaacctgac
     4801 cagttggctt tcgacaacag gcattattaa agccgctacc gatggcgtta gtaagaccct
     4861 gaataagtta accaaagact acaacgccgc cagcgatcgc attgatgcgc aggtcgcgcg
     4921 ctacaaagag caatttaccc aactggacgt tttaatgacc tcgttaaaca gcaccagcag
     4981 ctacttaacg cagcagttcg aaaacaacag taattccaag taatcaatat tcatcgggag
     5041 acaggtcatg tacgcggcaa aaggcaccca ggcctatgca caaattggcg tcgaaagcgc
     5101 cgtaatgagc gccagccagc aacagctggt caccatgcta tttgatggag tgctgagcgc
     5161 actggttaga gcgcgcctgt ttatgcagga caacaatcag caaggcaaag gcgtctcttt
     5221 gtcaaaagcg atcaacatca ttgagaacgg gctgcgggtg agtcttgatg aagagagcaa
     5281 agacgaacta acccaaaact tgattgctct ttatagctat atggtcaggc gcttgctgca
     5341 agccaattta cgcaacgatg tctccgcagt cgaagaagtg gaagcattaa tgcgcaatat
     5401 tgccgatgcc tggaaagagt ctttactctc cccttctttg attcaggacc cagactgatg
     5461 aacaatgcac cgcatttgta tttcgcctgg caacaactcg tcgaaaaaag tcagctcatg
     5521 ttacgcctgg cgacagaaga acaatgggac gaactcatcg ccagcgaaat ggcgtatgtg
     5581 aatgcggtgc aggagatcgc acacctgact gaagaggttg ccccgtccac cacgatgcag
     5641 gagcagctcc gcccgatgct gcgcctgatt ctcgacaacg aaagcaaggt aaagcagtta
     5701 ttacagattc ggatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg
     5761 gtgttaagtg cctatggcga tcagggtggc tttgtgctgg ctccgcagga taacctcttt
     5821 tgaatcttaa agagccaatg gctcgcgaat aatctgatta cggctacgct tctaatgttc
     5881 cccttgaatg gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt
     5941 acccggaagg cggtaagctc tggcctgaac tggccgagcg cgccgacggt tttaatgata
     6001 ttggtatcaa tatggtctgg ttgccgcccg cctataaagg cgcatcgggc gggtactcgg
     6061 tcggctacga ctcctatgat ttatttgatt taggcgagtt tgatcagaaa ggcagcatcc
     6121 ctactaaata tggcgataaa gcacaactgc tggccgccat tgacgccctg aaacgtaatg
     6181 acattgcggt gctgctggac gtggtagtca accacaaaat gggcgcggat gaaaaagaag
     6241 ctattcgcgt gcaacgtgta aatgctgatg accgtacgca aattgacgaa gaaatcattg
     6301 agtgcgaagg ctggacgcgt tacaccttcc ccgcgcgtgc cgggcaatac tcgcagttta
     6361 tctgggattt caaatgtttt agcggtatcg accatatcga aaaccctgat gaagatggca
     6421 tttttaaaat tgttaacgac tataccggag aaggctggaa cgatcaggtt gatgatgaac
     6481 tcggtaattt cgattatctg atgggtgaga atatcgattt tcgcaatcat gccgtgacgg
     6541 aagagattaa atactgggcg cgctgggtga tggaacaaac gcaatgcgac ggctttcgtc
     6601 ttgatgcggt caaacatatt ccggcatggt tttataaaga gtggatcgaa cacgtacagg
     6661 aagttgcgcc aaagccgctg tttattgtgg ctgaatactg gtcgcatgaa gtcgataaac
     6721 tgcaaacgta tattgatcag gtggaaggca aaaccatgct gtttgatgcg ccgctgcaga
     6781 tgaaattcca tgaagcatcg cgcatgggtc gcgactacga catgacgcag atcttcactg
     6841 ggacgctggt ggaagccgat ccttttcacg ccgtaaccct cgttgccaac cacgacaccc
     6901 aaccgttgca agccctcgaa gcaccggtcg aaccgtggtt taaaccgctg gcgtatgcct
     6961 taattttgtt gcgggaaaat ggcgttcctt cggtgttcta tccggacctc tacggtgcac
     7021 attacgaaga tgtcggtggt gacgggcaaa cctatccgat agatatgcca ataatcgaac
     7081 agcttgatga gttaattctc gcccgtcagc gtttcgccca cggtgtacag acgttatttt
     7141 tcgaccatcc gaactgcatt gcctttagcc gcagtggcac cgacgaatat cccggctgcg
     7201 tggtggtcat gtcgaacggg gatgatggcg aaaaaaccat tcatctggga gagaattacg
     7261 gcaataaaac ctggcgtgat tttctgggga atcggcaaga gagcgtagtg accgacgaaa
     7321 acggcgaagc gaccttcttt tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg
     7381 tgatttaaat tcatccccgg cggcaagccg gggagatttc attacggcag tggcgtcggt
     7441 aacggcgctt tatccagcgc ggcggcacac tctgccgtcg ggcgatccac acgctccatc
     7501 gtcataccat cgtactcaat ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttc
     7561 acggtaacgt tagtgagatc atcagacatc aatgtcaact tacccggcac ggcaatcacc
     7621 cgctgccact gacggcaatc cagggtatcg ccctctttag tgacaatcag gctgcctatc
     7681 gcttccggac tgaccagcgc actttgcggc cctttggtct gccagtagcc cgccagccag
     7741 tccggcgcgg gggttttcac gacattgtta tagttttcga cttcggcaca gcccgccagc
     7801 aacatgagcg cgcctgcaat tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag
     7861 aggattgtgg cattaaagcc ctgatgtcgc cagcctttcc aggttcagag agaaatttga
     7921 tctaactatt atttttgtga attattaccc cgaaaataaa tttgcttgat gtatgattcg
     7981 caactcttgt ttcaaacctt ctgagttcag aggctacata catgtcatgg cagcaattca
     8041 aacacgcctg gttgattaaa ttctgggccc ccatccctgc ggtcatcgcg gcgggtattc
     8101 tctccactta ctactttggc atcactggca ccttttgggc tgtcacgggt gaatttaccc
     8161 gctggggcgg tcagcttctg cagctgttcg gcgtccatgc tgaagagtgg ggttatttta
     8221 aaattatcca tctggaagga tcgccattaa cccgcatcga cggcatgatg atcctcggta
     8281 tgtttggcgg ctgctttgct gcagcgctgt gggccaacaa tgtcaaacta cgcatgccac
     8341 gcagccgtat ccgcattatg caggccatta ttggcggcat tatcgctggt tttggcgcgc
     8401 gtctggcaat gggctgtaac ctggcggcgt tctttaccgg cattcctcag ttctcgctgc
     8461 atgcctggtt ctttgccatc gccactgcca ttggttcatg gtttggcgcg cgctttaccc
     8521 tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga
     8581 cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg gatgctggtc tttttcggca
     8641 tgctgggctg ggcgctgctc acggcgatga accaaccaaa actggggctg gcaatgctgt
     8701 ttggcgtcgg ctttggttta ctgattgaac gtgcgcaaat ctgctttact tcggcgttcc
     8761 gcgatatgtg gatcaccgga cgtacccata tggcgaaagc aatcattatc ggtatggcgg
     8821 tgagtgccat cgggatcttc agttacgtac agttaggcgt tgaacctaaa atcatgtggg
     8881 cgggaccaaa tgcggtaatt ggtggtttac tttttggttt tggcatcgtg ctggcaggcg
     8941 gctgcgaaac cggctggatg taccgcgcgg tagaaggcca ggtgcactac tggtgggtcg
     9001 gtctgggcaa cgtgatcggc tcaacgattc tggcgtatta ctgggatgat ttcgctccgg
     9061 cgctggccac cgactgggac aaaatcaacc tgctgaaaac ctttggtccg atgggtggcc
     9121 tgctggtgac atatttgctg ttgtttgcag ctctaatgtt gattatcggc tgggaaaaac
     9181 gcttcttccg ccgtgcggca ccgcagattg ctaaggagat cgcatgaaaa atatcgttcc
     9241 tgattaccgt cttgatatgg tgggtgaacc ctgcccttat ccggctgtcg caacccttga
     9301 ggcgatgccg cagttaaaaa aaggggagat cctggaagtg gtgagcgact gtccgcagtc
     9361 gatcaataat attccactgg atgcgcgtaa tcacggctat acggtgctgg atattcagca
     9421 agacgggccg accattcgtt atttaattca gaagtaatcc ctcattcccg ctgtggccca
     9481 cagcgggaac atcgttacca ctctatcaca tagattttac ccgccccctc tcctattctt
     9541 ttcagtgtgc tctcaggagg tttctatgtg tggacgcttt gcccaatccc aaacgcgtga
     9601 agattacctc gcgtttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc
     9661 tattggcaga tacaacgtcg cgccgggaac caaagtcctt ctgctcagtg aacgtgatga
     9721 acaccttcat ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc
     9781 gctgattaac gcccgcgtag aaactgccgc aaccagtcgc atgtttaaac cgctctggca
     9841 acacggtcgg gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa
     9901 aaaacagcct tattttatct atcgcgctga cggacaacct gtttttatgg ccgcgatagg
     9961 tagcacaccg tttgagcgtg gtgacgaagc cgaaggattt ttgatcgtca ctgcggcggc
    10021 agatcaaggt ctggtagata ttcatgaccg ccgcccgctg gttctgtcgc cagaagctgt
    10081 acgggaatgg atgcggcaag aggttggcgg taaagaagcc tcagaaattg ctgccagtgg
    10141 ctgtgtgcca gcaaaccagt tcacctggca cccggtatcg tgcgcggtgg gtaatgttaa
    10201 aaaccaggga gcggcgttaa ttcaacctgt ttgataagcc tggagattat tgatcaacaa
    10261 tactgcgtca taagaaatct ctattagaca aagatttcag tacctgttgg catattgcaa
    10321 aaataacacc aatacggaat cgtcatgttc acgattaaaa cagatgatct cacccatcca
    10381 gcagtgcaag cattagtggc ttaccatatt tccggcatgc tgcagcagtc tccccccgaa
    10441 agtagtcatg ctttagatgt gcaaaaatta cgtaacccga aagtgacatt ctggtcagta
    10501 tgggaaggcg aacaactcgc aggaattggt gcgctgaagt tgctggatga taaacatggc
    10561 gaactgaaat caatgcgtac cgcgccaaat tatttacgtc ggggtgtcgc cagtctgatt
    10621 ttacgccaca ttttgcaggt cgcccatgac agatgccttc atcgcctgag cttagaaacg
    10681 ggtacacagg ctggatttac ggcctgccat caactttatt tgaaacatgg attcgttgat
    10741 tgcgaaccgt ttgctgatta tcaacttgat ccgcacagtc gatttttgtc attgacgcta
    10801 tgcgaaaata atgaattgcc atgagccaga cgcagcacat tcttgcattc gacgtgctgc
    10861 gcctttattt attatcagcc gcaaatgtct tgtccttaga cgctctttcc acatgcgcca
    10921 caagaaacct ctattccagt gacacaatta tgcctgatta tttccctagc ccggaatatg
    10981 aataaaaaca tcaaataaga tgaaattatc aaaatattaa tgttgcgtct gaggtcgtaa
    11041 caacatccga cgtggtgata tgtcaaatga gacactctga tggtcaaagg gtttatcgcg
    11101 tttacagatc gtggatggca gaaaactttc agaatcaggt ttccatttta aaccagaaat
    11161 taagtgagtt tgtcccatcc atgccccacg cggtgagatc ggatgtaatc aacaacaggt
    11221 tacaaaacct acacctgcat gctcatcact tcctgatacg ccgccaccag cttattacgc
    11281 acctgaatcc ccatttgcat tgaaactgag gctttttgca tatcggtcat cacatcgttt
    11341 aacgccacgc cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt
    11401 gtatcgctta tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt
    11461 ggcagtgatt cctgtgtacg cgcactcatc gccgtagcct gtaactggct gataacccct
    11521 tcaatcccct gtatcgctga cattttcatc tcccggataa tttctggcag caaagcctac
    11581 cagtaagtca ataagacaaa ggcgctaaat agcaacaaaa aaacgggttt attggcggat
    11641 agaaaaaaac gaaagcacaa ataatgggag cgtcaatttt tcgagtttgc tgacccggga
    11701 gtgagtcttg ttccactttg ccaataacgc cgtccataat cagccacgag gtgcgcgatg
    11761 aatgcgactg cagcccagac aaaatctctt gagtggctta atcgcctgcg tgcgaatccg
    11821 aaaattccat tgattgttgc cggctccgca gcagtggcgg tcatggtcgc actgatcctg
    11881 tgggcgaaag cccccgacta ccgcacatta ttcagcaatc tttccgatca ggatggtggc
    11941 gcaattgtca gccaactgac gcaaatgaat attccttacc gcttcagcga agccagcggc
    12001 gctattgaag ttccggcaga taaagttcac gaactgcgcc tgcgcctggc gcaacaaggt
    12061 ttgcccaaag gcggcgcggt cggtttcgaa ctgctcgatc aggaaaagtt tggtatcagc
    12121 cagttcagcg aacaggtgaa ttatcagcgg gcgctggaag gcgagctttc tcgtaccatc
    12181 gaaactatcg gcccggtaaa aggggcgcgc gtacatctgg caatgccgaa accgtcttta
    12241 ttcgtccgtg aacaaaaatc cccttctgca tcggtgacgg taaatctgtt acccggccgc
    12301 gcactcgatg aagggcaaat tagcgccatt gtacatctgg tttccagcgc ggttgctggt
    12361 ctgccgccgg ggaacgtcac gctggtggat cagggtggac atctgttaac ccagtccaat
    12421 accagcgggc gcgatcttaa tgacgctcag ttgaaatatg ccagcgatgt cgaaggccgg
    12481 attcagcggc gtattgaagc gatcctgtcg ccaattgttg gtaacggtaa tattcacgcc
    12541 caggtcacgg cgcaactgga cttcgccagt aaagaacaaa cagaagaaca gtatcgccct
    12601 aacggtgatg aatctcaggc ggcgcttcgt tcacgccagc ttaatgagag cgagcaaagc
    12661 ggttccggtt atccaggcgg cgtaccgggg gcgttgtcga atcaaccggc acctgcgaat
    12721 aacgcgccaa tcagcacgcc tccggcaaat caaaataacc gccagcagca ggcgagcacc
    12781 accagcaata gtgggccgcg tagcacacag cgtaatgaaa ccagtaacta cgaagtcgat
    12841 cgcaccattc gtcataccaa aatgaacgtg ggcgatgtgc aacgtctgtc agtcgcggta
    12901 gtggtgaatt acaaaacctt gccagacggt aaaccgttgc ctctcagcaa cgaacagatg
    12961 aagcaaattg aagatctgac ccgcgaggcg atgggctttt ctgaaaaacg cggtgactcg
    13021 ctcaatgtcg ttaactcgcc gttcaatagt agtgaggaaa gcggcggaga actgccgttc
    13081 tggcaacaac aagcgtttat cgatcagtta cttgctgccg gtcgctggtt gctggtactg
    13141 ctggtggcgt ggctgctgtg gcgtaaagcg gtacgcccgc agctaacacg tcgcgctgag
    13201 gcgatgaaag ctgtacagca acaggcgcag gcccgcgagg aagtggaaga tgcggtggaa
    13261 gtccgcctga gcaaagacga acaactccaa caacggcgcg ctaaccaacg tctgggggca
    13321 gaagtcatga gccagcgtat ccgtgaaatg tctgataacg atccgcgcgt ggtggcgctg
    13381 gtcattcgcc agtggataaa taacgatcat gagtaacctg acaggcaccg ataaaagcgt
    13441 catcctgctg atgaccattg gcgaagaccg ggcggcagag gtgttcaagc acctctccca
    13501 gcgcgaagtg caaactctga gcgctgcaat ggcgaacgtc acgcagatct ccaacaagca
    13561 gctaaccgat gtgctggcgg agtttgagca agaagctgaa cagtttgccg cactgaatat
    13621 caacgccaac gattatctgc gttcggtatt ggtcaaagct ctgggtgaag aacgtgccgc
    13681 cagcctgctg gaggatattc tcgaaacccg cgataccgcc agcggtattg aaacgctcaa
    13741 ctttatggag ccgcagagcg ccgccgatct gattcgcgat gagcatccgc aaattatcgc
    13801 caccattctg gtgcatctga agcgcgccca ggccgccgat attctggcgt tgttcgatga
    13861 acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt ggcggcgtgc agccagccgc
    13921 gctggcggaa ctgaccgaag tactgaatgg cttgctcgac ggtcagaatc tcaagcgcag
    13981 caaaatgggc ggcgtgagaa cggcagccga aattatcaac ctgatgaaaa cgcagcagga
    14041 agaagccgtt attaccgccg tgcgtgaatt cgacggcgag ctggcgcaga aaatcatcga
    14101 tgagatgttc ctgttcgaga atctggtgga tgtcgatgat cgcagcattc agcgtctgtt
    14161 gcaggaagtg gattccgaat cgctgctgat cgcgctgaaa ggggccgagc agccactgcg
    14221 cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat attctgcgcg acgatctcgc
    14281 caaccgtggt ccggtgcgtc tgtcgcaggt ggaaaacgaa cagaaagcga ttctgctgat
    14341 tgtgcgccgc cttgccgaaa ccggcgagat ggtgattggc agcggcgagg atacctatgt
    14401 ctgataatct gccgtggaaa acctggacgc cggacgatct cgcgccacca caggcagagt
    14461 ttgtgccaat ggtcgagtcg gaagaaacca ttattgaaga ggccgaaccc agccttgagc
    14521 agcaactggc gcaactgcaa atgcaggccc atgagcaagg ttatcaggcg ggtgttgccg
    14581 aaggtcgcca gcaaggtcat aagcagggct atcaggaagg actggcccag gggctggaac
    14641 aaggtctggc agaggcgaag tctcaacaag cgccaattca tgcccggatg cagcaactgg
    14701 tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt gattgcttcg cgcctgatgc
    14761 agatggcgct ggaggcggca cgtcaggtga tcggtcagac gccaacggtg gataactcgg
    14821 cactgatcaa acagatccaa cagttgttgc agcaagaacc gttattcagc ggtaaaccac
    14881 agctgcgcgt gcacccggat gatctgcaac gtgttgatga tatgctcggc gcaactttaa
    14941 gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca tcctggcggc tgtaaagtct
    15001 ccgccgatga aggcgatctc gacgccagtg tcgccactcg ctggcaagaa ctctgccgtc
    15061 tggcagcacc aggagtggtg taatgaccac gcgcctgact cgctggctaa ccacgctgga
    15121 taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt cgctacgggc gattaacccg
    15181 cgctaccggg ctggtgctgg aagccaccgg attacaattg ccgctcggcg caacctgtgt
    15241 cattgagcgc cagaacggca gcgaaacgca cgaagtagaa agcgaagtcg ttggctttaa
    15301 cggtcaacgg ctgtttttaa tgccgctgga ggaagtcgaa ggtgtcctgc ccggcgcgcg
    15361 tgtttatgcc aaaaacattt cggctgaagg gctgcaaagc ggcaagcagt tgccgctcgg
    15421 ttcggcgtta ttaggtcgcg ttctggacgg cagcggtaaa ccgcttgatg gcctcccctc
    15481 ccccgatacg acggaaaccg gtgcgctgat taccccgcca tttaacccgt tgcaacgtac
    15541 accgattgaa catgtgctgg acaccggcgt gcgcccaatc aatgccctgc ttaccgtcgg
    15601 gcgcgggcag cgtatggggc tgtttgccgg gtccggcgtt ggtaaaagtg tgctgctggg
    15661 gatgatggca cgttacaccc gcgccgatgt cattgtcgtg ggtttgattg gtgaacgagg
    15721 gcgcgaagta aaagatttta ttgagaatat cctcggtgcc gaaggacgtg cacgctccgt
    15781 ggtgattgcc gctccggcgg atgtttctcc gctcctgcga atgcagggtg ccgcctatgc
    15841 cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat gtgttgctga ttatggactc
    15901 cctcacccgc tacgcgatgg cccagcgtga aattgcgctg gcgattggcg aaccacctgc
    15961 cactaaaggt tatccaccgt cggtgtttgc caaattaccg gcactggtcg agcgtgccgg
    16021 aaatggcatt agcggcggcg gctcgattac cgcgttttat accgtgctca ccgaaggcga
    16081 tgaccagcag gaccccattg ccgactccgc gcgggcaatc ctcgacggtc acattgtgct
    16141 gtctcgccga ctggcggaag ccgggcacta tccggctatc gatattgaag cgtcgatcag
    16201 tcgcgcaatg acggcgttga tcagtgagca acattacgcg cgagtgcgca ccttcaaaca
    16261 gctgttgtcg agttttcagc gtaaccgcga tctggttagc gtcggcgcgt atgccaaagg
    16321 cagcgatccg atgctcgaca aagccatcgc cctgtggccg cagctggagg gctatttgca
    16381 acaaggcatt tttgaacgcg cggactggga agcgtctctc caggggctgg agcgtatttt
    16441 cccgacagta tcataaccca ggagataacg gcagatgaca gaacatggcg cgctggcgac
    16501 cctgaaagat ctggcagaaa aagaggtaga ggatgccgcg cgcctgctgg gtgaaatgcg
    16561 tcggggatgt cagcaggcgg aagaacagct caaaatgctg atcgattatc agaatgaata
    16621 tcgcaataac ctcaatagcg atatgagtgc cgggatgacc agcaaccgct ggatcaacta
    16681 tcagcagttt atccagacgc tggaaaaagc cattactcag catcgccagc aacttaatca
    16741 gtggacgcag aaagtagaca ttgccctgaa cagctggcga gaaaagaaac aacgtttgca
    16801 ggcttggcag acactgcagg aacggcaatc cacggcggca ctgcttgcag aaaaccgcct
    16861 cgatcagaaa aagatggatg agttcgccca gcgcgccgcc atgaggaaac ctgaatgatt
    16921 cgcatagcgc ccttgattac cgctgacgtt gataacgcca cattgcctgg cggcaaagcc
    16981 agcgatgctg cacaagattt tctcgcgttg ttgagcgaag cattagcagg cgagacaaca
    17041 accgacaaag cggcccccca gttgctggtg gcaacagata agcccacgac aaaaggcgag
    17101 ccgctggtca gcgatattgt ttccgatgcg caacaagcag atttactgat ccctgtggat
    17161 gaaacaccgc ctgtcatcaa cgacaaacaa tccacatcaa catcgttaac caccgctcag
    17221 acgataacgt tggctgcggc ggctgacaac aatacggcaa aagacgaaaa agcggatgat
    17281 ctgaatgaag acgtcaccgc aagcctgagc gccctttttg cgatgttgcc gggttttgac
    17341 aatacgccca aagtgactga tgcaccgtca accgtgttac cgacagagaa accaacgctt
    17401 ttcacaaaac tgacttctgc gcaactcact acagcacagc ctgatgacgc ccccggcaca
    17461 ccagctcagc cattaacacc gctggtagca gaagcccaga gtaaagcgga agtcatcagc
    17521 acgccttcgt cggtgaccgc tgccgccagc ccgctaatca ctccacacca gacacagcca
    17581 ctgcccaccg tcgccgcgcc tgttttgagt gcaccgctgg gttctcacga atggcaacaa
    17641 tcattaagcc agcatatttc gctgttcacc cgccaggggc aacaaagtgc agagttgcgt
    17701 ctgcacccac aggatttagg tgaagtgcaa atttccctca aagtggatga taaccaggcg
    17761 caaatccaga tggtttcacc gcatcagcac gtacgcgccg ccctggaagc agcgctgccg
    17821 gtactgcgta cgcagctggc cgaaagtggc attcagttag ggcaaagcaa catcagtggc
    17881 gaaagcttta gtggtcagca gcaggccgct tcccaacaac agcaaagcca acgcacagca
    17941 aaccatgaac ctctggcggg ggaagacgac gatacgcttc cggttcccgt ctctttacaa
    18001 gggcgtgtaa caggcaacag cggcgttgat attttcgcct aacgtcagag gtagcaccgt
    18061 aatccgcgtc ttttccccgc tttgttgcgc tcaagacgca ggataattag ccgataagca
    18121 gtagcgacac aggaagaccg caacacatga ctgattacgc gataagcaag aaaagcaagc
    18181 gatcgctttg gatcccgatt ctggtattca ttaccctcgc ggcctgtgcc agcgcaggtt
    18241 acagctactg gcattcgcat caggttgccg ctgaagacaa agcgcagcaa cgcgtcgtgc
    18301 cctccccggt cttctatgcg ctggatacct ttacggtcaa tctgggcgat gcggatcgcg
    18361 tactttatat cggcataacc ctgcgactca aagatgaagc tacccgctcg cggctgagtg
    18421 agtatttgcc ggaagtccgt agccgtttgc tgttactgtt ttcgcgtcag gatgctgccg
    18481 tactggcgac agaagaaggt aagaaaaacc tgattgccga gattaaaacc acactttcca
    18541 ccccgcttgt tgccgggcaa ccgaaacagg atgtcaccga cgtgctgtat accgctttta
    18601 ttctgcgata acgacatggg cgatagtatt ctttctcaag ctgaaattga tgcgctgttg
    18661 aatggtgaca gcgaagtcaa agacgaaccg acagccagtg ttagcggcga aagtgacatt
    18721 cgtccgtacg atccgaatac ccaacgacgg gttgtgcgcg aacgtttgca ggcgctggaa
    18781 atcattaatg agcgctttgc ccgccatttt cgtatggggc tgttcaacct gctgcgccgt
    18841 agcccggaca ttaccgtcgg ggccatccgc attcagccgt accatgaatt tgcccgcaac
    18901 ttgccggtgc cgaccaacct gaaccttatc caccttaaac cgctgcgcgg cacagggctg
    18961 gtggtgttct caccgagtct ggtgtttatc gccgtggata acctgtttgg cggcgatgga
    19021 cgcttcccga ccaaagtgga aggtcgcgag tttacccata cagaacagcg cgtcatcaac
    19081 cgcatgttaa agctggcgct tgaaggctat agcgacgcct ggaaggcgat taatccgctg
    19141 gaagttgagt acgtacgttc ggaaatgcag gtgaaattta ccaatatcac cacctcaccg
    19201 aacgacatcg tggttaacac gccgttccat gtggagattg gcaacctgac cggcgaattt
    19261 aatatctgtc tgccattcag catgatcgag ccgctacggg aattgttggt taacccgccg
    19321 ctggaaaact cgcgtaatga agatcagaac tggcgcgata acctggtgcg ccaggtgcag
    19381 cattcacagc tggagctggt cgccaacttt gccgatatat cgctacgcct gtcgcagatt
    19441 ttaaaactga aacccggtga cgtcctgccg atagaaaaac ccgaccgcat catcgcccat
    19501 gttgacggtg tcccggtgct gaccagtcag tacggcaccc tcaacggtca gtatgcgtta
    19561 cggatagaac atttgattaa cccgatttta aattctctga acgaggaaca gcccaaatga
    19621 gtgacatgaa taatccggcc gatgacaaca acggcgcaat ggacgatctg tgggctgaag
    19681 cgttgagcga acaaaaatca accagcggca aaagcgctgc cgacgcggtg ttccagcaat
    19741 ttggcggtgg tgatgtcagc ggaacgttgc aggatatcga cctgattatg gatattccgg
    19801 tcaagctgac cgtcgagctg ggccgcacac gaatgaccat caaagagtta ttgcgtctga
    19861 cgcaagggtc cgtcgtggcg ctggacggcc tggcgggcga accgctggat attctgatca
    19921 acggttattt aattgctcag ggcgaagttg tcgtagtcgc tgataaatat ggcgtgcgga
    19981 tcaccgatat cattactccg tctgagcgaa tgcgtcgcct gagccgttag tgatgaataa
    20041 ccatgctact gtgcaatctt ccgcgccggt ttctactgcg ccactgctgc aggtgagcgg
    20101 cgcactcatc gccattattg ccctgatcct cgctgctgcc tggctggtaa aacggctggg
    20161 atttgccccg aaacgcactg gcgttaacgg tctgaaaatt agcgccagtg cttcgctggg
    20221 cgcgcgtgaa cgggtcgtgg tggtcgatgt ggaagatgca cggctggtgc tcggcgttac
    20281 cgcagggcaa atcaatctgc tgcataaact tcccccttca gcaccaacgg aagagatacc
    20341 gcagaccgat tttcagtcgg tcatgaaaaa cttgcttaag cgtagcggga gatcctgatg
    20401 cgtcgtttgt tgtctgtcgc acctgtcctt ctctggctgg ttacacccct cgccttcgcg
    20461 caactgacgg gtattaccag ccagccgctg cctggcggtg gacaaagctg gtcgctcccg
    20521 gtgcagacgc tggtgttcat tacctcgttg acgtttattc cggcaatttt actgatgatg
    20581 accagtttca cccgcatcat cattgttttt ggtttattgc gtaacgcact gggaacaccc
    20641 tccgcgccac ctaaccaggt attgctgggg ctggcactgt ttttgacctt ttttattatg
    20701 tcaccggtga tcgataaaat ttatgtcgat gcgtaccagc cattcagcga agagaaaatc
    20761 tcaatgcagg aggcgctgga aaaaggggcg cagccgctgc gtgagtttat gctgcgtcag
    20821 acccgtgagg cggatttagg attgtttgcc agactggcaa atactggccc gttgcaggga
    20881 cctgaagccg tgccgatgcg cattttgctc ccggcttacg tgaccagcga gttgaaaacc
    20941 gcatttcaga taggcttcac gatcttcatt ccgtttttga ttatcgacct ggtgattgcc
    21001 agcgtgttga tggcattggg gatgatgatg gtcccccccg ccaccattgc tctgcccttt
    21061 aaactgatgc tgtttgtact ggtggatggc tggcaattgc tggtcggttc gctggcgcag
    21121 agcttttaca gctagagagg caaaatgaca cctgaatcgg tcatgatgat ggggactgaa
    21181 gcgatgaaag tcgcgctggc actggctgcc ccgctattgc tggtagcatt ggtcacgggc
    21241 cttatcatca gtattttgca ggccgccacg cagattaacg aaatgacgct gtcgtttatt
    21301 ccgaaaatca tcgccgtatt tatcgccatt attattgccg gaccgtggat gctcaatctg
    21361 ttgctggatt acgtccgcac cttgttcact aacctgccgt atatcatcgg gtaggccgta
    21421 caatgatgca ggtgacaagc gatcaatggc tttcctggct aagtctgtac ttctggccgt
    21481 tactgcgcgt gttggcgctg atctccaccg cgccgattct gagcgaacgc agcgtaccga
    21541 aacgggtaaa actgggtctg gcaatgatga tcacgttcgc tattgcccca tcattacctg
    21601 ccaacgatgt tcctgttttt tcgttctttg ctctgtggct ggccgtgcag cagatcctga
    21661 tcggcattgc gcttggtttt accatgcaat ttgcctttgc cgcggtgcga accgctggtg
    21721 aaattatcgg tctgcaaatg gggctgtcat ttgcgacgtt tgtcgatccg gccagccatc
    21781 ttaatatgcc cgttttagcg cgtatcatgg atatgctggc gttactgctg ttcctgacat
    21841 ttaacggtca tttatggttg atttcactgc tggtcgatac ctttcacacc ctgccaattg
    21901 gtggcgaacc gttgaacagc aatgcgtttc tggcactcac caaagcaggg agtttgattt
    21961 tccttaacgg gctgatgctg gcgttaccgc tcattactct gctgctgacc ctgaatctgg
    22021 cattaggttt actaaatcgt atggccccgc aattatccat ttttgttatt ggatttccat
    22081 taactctgac tgtcggtatc tctttaatgg cggcattaat gccgttaatt gcgccttttt
    22141 gcgaacattt attcagtgag atgtttaatt tgctggctga tattattagt gaattgccat
    22201 taatataatt ccgtaacgtt tatcatgcta ccctaaggat tatccgaaaa ataataccta
    22261 cgaacatctt ccaggatact cctgcggcga aatatttgtt ttaagctcac tcacatatcg
    22321 caacatttac tttactttaa gacaattcca ggcaaattat acaacacttt acgggatagt
    22381 aagtccgcct gaaaaatcgc gagagtggcg cattaggtga cccatgttgt tccgtttagt
    22441 catgatgaaa tattcaggta aggggaatta tcgttacgca ttgagtgagg gtatgccatg
    22501 tcaacgatta ttatggattt atgtagttac acccgactag gtttaaccgg gtatctgttg
    22561 agtagagggg ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga ccttgccata
    22621 gcttgtgatt cacagcgccc ttcagtggtg tttattaatg aggactgttt catccacgat
    22681 gcttctaaca gtcagcgcat caagcacatc attaatcaac atcccaatac gttatttatc
    22741 gtttttatgg caattgccaa tgttcatttt gatgaatatc tattggtcag aaaaaattta
    22801 ttgatcagtt ctaaatcgat taaaccggaa tctctcgatg atatccttgg cgatattctg
    22861 aaaaaagaga caacgataac ctcgttttta aatatgccga cgttatcatt gagccgaacc
    22921 gaatcgagta tgttgcgaat gtggatggca ggtcagggaa ccattcaaat ctccgaccaa
    22981 atgaatatca aagccaagac
//
LOCUS       DAZGAP010000078        22968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGAP010000078 DAZGAP010000000
VERSION     DAZGAP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.078"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..441
                     /locus_tag="ACHLAM_002564"
     CDS             <1..441
                     /locus_tag="ACHLAM_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7854073.1"
                     /translation="RFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVE
                     QELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPE
                     LMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
     gene            702..1043
                     /gene="raiA"
                     /locus_tag="ACHLAM_002565"
     CDS             702..1043
                     /gene="raiA"
                     /locus_tag="ACHLAM_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO7854074.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    1146..1271
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            1147..1194
                     /gene="pheL"
                     /locus_tag="ACHLAM_002566"
     CDS             1147..1194
                     /gene="pheL"
                     /locus_tag="ACHLAM_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO7854075.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            1293..2453
                     /gene="pheA"
                     /locus_tag="ACHLAM_002567"
     CDS             1293..2453
                     /gene="pheA"
                     /locus_tag="ACHLAM_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO7854076.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2496..3617)
                     /gene="tyrA"
                     /locus_tag="ACHLAM_002568"
     CDS             complement(2496..3617)
                     /gene="tyrA"
                     /locus_tag="ACHLAM_002568"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO7854077.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3628..4698)
                     /gene="aroF"
                     /locus_tag="ACHLAM_002569"
     CDS             complement(3628..4698)
                     /gene="aroF"
                     /locus_tag="ACHLAM_002569"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO7854078.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4908..5273
                     /gene="yfiL"
                     /locus_tag="ACHLAM_002570"
     CDS             4908..5273
                     /gene="yfiL"
                     /locus_tag="ACHLAM_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO7854079.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            5423..5941
                     /gene="yfiR"
                     /locus_tag="ACHLAM_002571"
     CDS             5423..5941
                     /gene="yfiR"
                     /locus_tag="ACHLAM_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO7854080.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5931..7157
                     /gene="dgcN"
                     /locus_tag="ACHLAM_002572"
     CDS             5931..7157
                     /gene="dgcN"
                     /locus_tag="ACHLAM_002572"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO7854081.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            7173..7655
                     /gene="yfiB"
                     /locus_tag="ACHLAM_002573"
     CDS             7173..7655
                     /gene="yfiB"
                     /locus_tag="ACHLAM_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7854082.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     ALSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7732..8079)
                     /gene="rplS"
                     /locus_tag="ACHLAM_002574"
     CDS             complement(7732..8079)
                     /gene="rplS"
                     /locus_tag="ACHLAM_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO7854083.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(8121..8888)
                     /gene="trmD"
                     /locus_tag="ACHLAM_002575"
     CDS             complement(8121..8888)
                     /gene="trmD"
                     /locus_tag="ACHLAM_002575"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO7854084.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8919..9467)
                     /gene="rimM"
                     /locus_tag="ACHLAM_002576"
     CDS             complement(8919..9467)
                     /gene="rimM"
                     /locus_tag="ACHLAM_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO7854085.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9486..9734)
                     /gene="rpsP"
                     /locus_tag="ACHLAM_002577"
     CDS             complement(9486..9734)
                     /gene="rpsP"
                     /locus_tag="ACHLAM_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO7854086.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9871..11232)
                     /gene="ffh"
                     /locus_tag="ACHLAM_002578"
     CDS             complement(9871..11232)
                     /gene="ffh"
                     /locus_tag="ACHLAM_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO7854087.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11399..12190
                     /gene="ypjD"
                     /locus_tag="ACHLAM_002579"
     CDS             11399..12190
                     /gene="ypjD"
                     /locus_tag="ACHLAM_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO7854088.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            12211..13497
                     /gene="yfjD"
                     /locus_tag="ACHLAM_002580"
     CDS             12211..13497
                     /gene="yfjD"
                     /locus_tag="ACHLAM_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO7854089.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13551..14144)
                     /gene="grpE"
                     /locus_tag="ACHLAM_002581"
     CDS             complement(13551..14144)
                     /gene="grpE"
                     /locus_tag="ACHLAM_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO7854090.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            14267..15145
                     /gene="nadK"
                     /locus_tag="ACHLAM_002582"
     CDS             14267..15145
                     /gene="nadK"
                     /locus_tag="ACHLAM_002582"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO7854091.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            15231..16892
                     /gene="recN"
                     /locus_tag="ACHLAM_002583"
     CDS             15231..16892
                     /gene="recN"
                     /locus_tag="ACHLAM_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO7854092.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKHLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            17041..17382
                     /gene="bamE"
                     /locus_tag="ACHLAM_002584"
     CDS             17041..17382
                     /gene="bamE"
                     /locus_tag="ACHLAM_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO7854093.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(17444..17734)
                     /gene="yfjF"
                     /locus_tag="ACHLAM_002585"
     CDS             complement(17444..17734)
                     /gene="yfjF"
                     /locus_tag="ACHLAM_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO7854094.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17724..18161)
                     /gene="ratA"
                     /locus_tag="ACHLAM_002586"
     CDS             complement(17724..18161)
                     /gene="ratA"
                     /locus_tag="ACHLAM_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO7854095.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            18332..18814
                     /gene="smpB"
                     /locus_tag="ACHLAM_002587"
     CDS             18332..18814
                     /gene="smpB"
                     /locus_tag="ACHLAM_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO7854096.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            19029..19391
                     /gene="ssrA"
                     /locus_tag="ACHLAM_002588"
     tmRNA           19029..19391
                     /gene="ssrA"
                     /locus_tag="ACHLAM_002588"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(19552..>22968)
                     /gene="ypjA"
                     /locus_tag="ACHLAM_002589"
     CDS             complement(19552..>22968)
                     /gene="ypjA"
                     /locus_tag="ACHLAM_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO7854097.1"
                     /translation="TGTNINSGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISA
                     GGSLIVYTGGIAHGVNQETGSALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTG
                     ELTVMAKTSAKNTTIDAGGKLIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGA
                     LIASTTSGTLIEGTNSYGDAFYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKM
                     DVYGKDVGTVLNSAGTQTIYASATSDKANIKGGKQTVYGLATDANIESGEQIVDGGST
                     DKTHINGGTQTVQNYGKAINTDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSV
                     LNDGGTLDVREKGSATGIQQSSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLT
                     NGGVLTVESDTTSAKTQVNAGGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGI
                     QTVSANGEAIKTTINEGGTLTVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTF
                     SIAGYLATNVLLENGGNLLVLAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGR
                     SKDEFQVLARAEDLQVAGGTAIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAV
                     RITDSATLTLGNGVDTTLADLTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVY
                     LSAQTAAPATTNGIYNTLTTSELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQ
                     DTGVSPQSDDAMTLVKTGGGDASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKP
                     NPDPNPNPKPDPKPDPKPDPKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDA
                     ELNSIRERLNIMKASPHNNNVWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIP
                     EGIATLGAFMGYSHTHIFFDRGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESN
                     VAGKMSSGGAANGSYRSNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNG
                     MESKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFV
                     NDLSGRRGIYQAGIKASFSSSLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
BASE COUNT         5764 a         6063 c         5409 g         5732 t
ORIGIN      
        1 cgcttccccg gaggagaaat ggcggatctg tacagtcatc gctgggaaat cgaactggga
       61 tacagggaga taaaacagac gatgcaactg agcaggctga cgctgagaag taaaaagccg
      121 gagcttgtgg agcaagagct gtggggtgtc ttactggctt ataatctggt gagatatcag
      181 atgattaaaa tggcggaaca tctgaaaggt tactggccga atcaactgag tttctcagaa
      241 tcatgcggaa tggtgatgag aatgctgatg acattgcagg gcgcttcacc gggacgtata
      301 ccggagctga tgcgcgatct tgcaagtatg ggacaacttg tgaaattacc gacaagaagg
      361 gaaagggcct tcccgagagt ggtaaaggag aggccctgga aataccccac agccccgaaa
      421 aagagccagt cagttgctta actgatcggc attacagccc ttgagctgcc gtttttttat
      481 tctgttagtt gtgaaactga agcgatttag tcactatcga tctcatcaaa tatggctcgc
      541 tttgagatat tcctcaagta aaaaaacacc tcttcctgcg atttctcaca aaaaagattc
      601 gttgacaaaa agtgacaaaa ttatgagatt tccatcacac attttgacat caggaacggt
      661 atgctgaatt caccaagacg ggaagacaag aggtaaaatt tatgacaatg aacattacca
      721 gcaaacaaat ggaaattact ccggccatcc gccaacatgt cgcagaccgt ctcgccaaac
      781 tggaaaaatg gcaaacacat ctgattaatc cacatatcat tctgtccaaa gagccacaag
      841 ggtttgttgc tgacgccaca atcaatacac ctaacggcgt tctggttgcc agtggtaaac
      901 atgaagatat gtacaccgca attaacgaat tgatcaacaa gctggaacgg cagctcaata
      961 aactgcagca caaaggcgaa gcacgtcgtg ccgcaacatc ggtgaaagac gccaacttcg
     1021 tcgaagaagt tgaagaagag tagtccttta tattgagtgt atcgccaacg cgccttcggg
     1081 cgcgtttttt gttgacagcg tgaaaacagt acgggtactg tactaaagtc acttaaggaa
     1141 acaaacatga aacacacacc gtttttcttc gcattctttt ttaccttccc ctgaatggga
     1201 ggcgtttcgt cgtgtgaaac agaatgcgaa gacgaacaat aaggcctccc aaatcggggg
     1261 gcctttttta ttgataacaa aaaggcaaca ctatgacatc ggaaaacccg ttactggcgc
     1321 tgcgagagaa aatcagcgcg ctggatgaaa aattattagc attactggca gagcgacgcg
     1381 aactggccgt cgaggtggga aaagccaaac tgctctcgca tcgcccggta cgtgatattg
     1441 atcgtgaacg cgatttgctg gaaagattaa ttacgctcgg taaagcgcac catctggacg
     1501 cccattacat tactcgcctg ttccagctca tcattgaaga ttccgtatta actcagcagg
     1561 ctttgctcca gcaacatctc aataaaatta atccgcactc agcacgcatc gcttttctcg
     1621 gccccaaagg ttcttattcc catcttgcgg cgcgccagta tgctgcccgt cactttgagc
     1681 aattcattga aagtggctgc gccaaatttg ccgatatttt taatcaggtg gaaaccggcc
     1741 aggccgacta tgccgtcgta ccgattgaaa ataccagctc cggtgccata aatgacgttt
     1801 acgatctgct gcaacatacc agtttgtcga ttgttggcga gatgacgtta actatcgacc
     1861 attgtttgtt ggtctccggc actactgatt tatccaccat caatacggtc tacagccatc
     1921 cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta tccgcaatgg aagattgaat
     1981 ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca ggcaaaatca ccgcatgttg
     2041 ctgcgttggg aagcgaagct ggcggcactt tgtacggttt gcaggtactg gagcgtattg
     2101 aagcgaatca gcgacaaaac ttcacccgat ttgtggtgtt ggcacgtaaa gccattaacg
     2161 tgtctgacca ggttccagcg aaaacgacgt tgttaatggc gaccgggcaa caagctggtg
     2221 cgctggttga agcgttgctg gtactgcgca accacaatct gattatgacc cgtctggagt
     2281 cacgcccgat tcacggtaat ccatgggaag agatgtttta tctggatatt caggccaatc
     2341 ttgaatcagc ggaaatgcaa aaagcattga aagagttagg ggacatcacc cgttcgatga
     2401 aggtattggg ctgttaccca agtgagaacg tagtgcctgt tgatccaacc tgatgaaaag
     2461 gtgccggatg atgtgaatca cccggcactg gattattagt gacggttgtc attcgcctga
     2521 cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt aatcgccgaa ccagtgctcc
     2581 accttgcgga aactgtcaat aaacgcctgc ttatcgccct gctccagcaa ctcaatcgcc
     2641 tcgccgaaac gcttatagta acgtttgatt aacgccagat tacgctctga cgacataata
     2701 atgtcggcat acagttgcgg atcctgggca aacagtcgcc caaccatcgc cagctcaagg
     2761 cggtaaatcg gcgaagaaag cgccagaagt tgctcaagct gaacattttc ttctgccagg
     2821 tgcagcccat aagcaaaagt agcaaagtgg cgcagtgcct gaataaacgc catattctga
     2881 tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc agacctgaat ttgctccaga
     2941 aaccattggt atgcttccgg cttacgtcca tcacaccaga ccacaacttg ttttgccagg
     3001 ctaccgctgt ccgggccgaa catcgggtgt aaccccagca ccggaccatc atgcgccgcc
     3061 agcatggcct gtaatggtcc atttttcacc gatgccagat cgaccagaat acaatctttc
     3121 ggtaaaggcg gtaatttgcc aataacttgt tcagtaacgt ggatgggcac actaacaatc
     3181 accattccgg catcggcaac tatctcagcc gctcgatccc agtcatgttg ctccagaatc
     3241 cgcacctgat aacccgatag tgtcagcatc ttctcgaaca ggcgtcccat ctgaccgcca
     3301 ccgccgacga taaccaccgg gcgcagtgac ggacaaagcg ttttaaatcc tttgtcgttt
     3361 tcactggagt aagattcacg catcacccga cgcaaaacat cctcaatcag atctggcggt
     3421 acacccagag cttccgcctc tgcacgacgc gaggccaaca tagatgcctc gcgctccgga
     3481 acataaatag gcagtccaaa gcggcttttc acctcgccca cttcagcaac cagttccaga
     3541 cgcttcgcta ataaattcag cagcgcttta tcgacttcat caatttgatc gcgtaatgcg
     3601 gtcaattcag caaccataat aaacctctta agccacgcga gccgtcagct gcccgttcag
     3661 atcctgatga atttcacgca gcaaggcatc ggtcatttcc cagctaatgc aggcatcggt
     3721 tacggataca ccgtatttca tttcactgcg cggttgctcg gaagactgat tgccctcgtg
     3781 gatattactt tcgatcatca gaccaataat tgagcgattg ccatctttga tttgagcaac
     3841 cacggattct gccaccgcag gctgacggcg ataatcttta ttggaattac cgtggctgca
     3901 atctaccatc agagacgggc gcagtcccgc ctgttccatc tctttttcac attgcgcaac
     3961 atccgcaggg ctatagttcg gcgctttacc accgcgcagg atcacatgcc cgtccggatt
     4021 cccctgagtt tgtagcaacg ccacctgccc tgcctggtta atgccaacaa aacggtgcgg
     4081 ctgggcggcg gcacgcatag cgttaatagc cgttgcaagg ctgccgtcgg tgccgttttt
     4141 aaaaccaacc ggcatggaaa gcccggaggc catttcacgg tgtgtttgcg attccgttgt
     4201 acgagcacca attgctgacc agctaaacag atcgcccagg tattgcgggc tattcggatc
     4261 taacgcttcc gtcgccagtg gcagtcccat attcaccagc tcaagcagca atttacgcgc
     4321 gatctgcagc ccggcttcta catcaaaaga gccatccata tggggatcgt taatcaaccc
     4381 tttccaaccg acagtggtac ggggtttttc aaaatagacg cgcattacca gatagaggct
     4441 atcgctgacc tctgcggcaa gggctttaaa tcgacgagca tattccagag cagtttccgg
     4501 atcatgaatg gaacaaggac cacatactac caacagacga ggatcgcgcc cggcgataat
     4561 atctgaaatg gttttacgcg agtcagcaat ctgggcttcc tgttgcaggc ttaatggaaa
     4621 agcggccttc agttgttccg gagtcattaa aacttgttcg tcggtaatat gtacgttatt
     4681 cagcgcgtct ttttgcatga tggcgatcct gtttatgctc gtttgcgata gttgatcctc
     4741 agcgaggatg acgtaacgat aacacataaa gtaaagtttt caatccatat ttcgtacatt
     4801 tttctttaca caagcaattt attcgcgctt ttaacccttg tttatgtata aataaattta
     4861 caccaccttt gaaaacaatc cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt
     4921 ttatcgcccc cttgttggct ttactggtta gcggatgtca gattgatcct tatactcacg
     4981 cgccaacctt gaccagcacc gactggtatg atgttggtat ggaagatgcg atatcgggca
     5041 acgccataaa agatgacgat gcatttagcg attcacaggc ggatcgcggt ctatacctta
     5101 aaggatatgc cgaaggacaa aagaaaactt gccagaccga ttttacttat gcccgaggac
     5161 tttccggtaa aagctttcct gccagctgca acaatgttga aaatgccagc caactgcatg
     5221 aagtatggca aaaaggggct gatgaaaatg ccagcacaat acgattaaat taattaaacc
     5281 cccacaaata aattaaacat gagattctac tcattgtgtt ttatttctca cattgatgac
     5341 gaccgcatga aagaatgata ttttaatgag cattctgaaa aaatatcgat gcatttcgag
     5401 cgaagatggt gaggatccct gaatgcgttt ttctcaccga ctgttcttac tgctgattct
     5461 cctgctgacg ggagcaccaa tactcgctca ggaaccttct gacgttgcga aaaacgtgcg
     5521 tatgatggtt tccggtattg tcagttacac ccgctggcct gcgctatcag ggccaccaaa
     5581 gttatgtatc ttttcatctt cacattttag taacgcccta caagagaatg gcacgacatc
     5641 tttgccttat ctacctgtca tcattcacac tcaacaagag gcgatgattt caggctgcaa
     5701 tggtttttat tttggcaacg agtcaccgac attccagatg gaattaaccg aacaatatcc
     5761 gtcaaaggcg ttattattaa ttgccgaaca aaataccgag tgcattattg gtagcgcctt
     5821 ttgtctaatc atccacaata atgacgtcag atttgccgta aacctggatg ccttatcgcg
     5881 tagcggtgta aaggtcaacc cggatgtctt aatgctcgca cggaagaaaa atgatggata
     5941 acgataattc tcttaataag cgccccacgt ttaaaagagc attacgcaac atcagtatga
     6001 ccagcatatt tatcactatg atgctgatct ggttgctgct ttccgtgacc tcggtgctga
     6061 ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc agcaacaatg acttacagtc
     6121 tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac tgaaacactg gcagcgctgg
     6181 gccagcaagg ccaattttca actgcagaag tgcgtgataa gcagcaaaat attctggcat
     6241 cctggcatta cacccataag gaaccaggcg atactttcag caatttcata agccactggc
     6301 tcttccctgc ccccatcatt cagccgattc gtcacaatgg tgaaaccatt ggcgaagtac
     6361 gcttaactgc tcgcgacagt tcaatcagcc attttatctg gttttcgctc gctgtcctga
     6421 ccggttgtat tctgctggca tcaggcatcg caattaccct cacccgccat ttgcacaatg
     6481 gcctggtgga agcgctgaaa aatatcaccg atgtcgtaca tgatgtgcgt tctaaccgca
     6541 atttttcccg acgagtttcg gaagaacgta tcgctgagtt tcaccgcttc gctctcgact
     6601 tcaacagtct gctggatgaa atggaagagt ggcagcttcg tttacaggcc aaaaatgcgc
     6661 agcttttacg taccgcgcta catgacccat taaccgggct ggctaaccgc gcagcgtttc
     6721 gtagcggcat caacacgttg atgaacaatt ccgatgcccg aaaaacgtcg gcgttactat
     6781 ttcttgatgg cgataatttc aaatatatca atgatacctg gggtcatgcg acgggcgata
     6841 gagtcttgat tgaaatcgca aaacggttag ctgaatttgg cggactgcga cataaagcat
     6901 accgcctggg cggcgatgaa ttcgcgatgg tgctctatga tgtacagtcg gaatctgaag
     6961 tgcagcagat atgctcagca ctgacacaaa tctttaatcg cccgtttgat cttcataatg
     7021 gtcatcagac caccatgaca ttaagcattg gttacgcgat gaccattgag catgcctctg
     7081 cggaaaaatt acaagagctg gccgatcaca atatgtatca ggccaaacac cagcgtgccg
     7141 aaaagctggt gagataacaa ggatatatcg atatgataaa gcacctggta gcacccctga
     7201 ttttcacctc actaatactg actggctgcc agtcccctca gggaaagttt actcctgagc
     7261 aagtcgccgc tatgcaatct tatggattta ctgaatccgc cggcgactgg tcgctgggct
     7321 tatcagatgc cattctgttc gcaaaaaatg actacaaatt gctcccggaa agccagcaac
     7381 agatccaaac catggcagcc aaattggcct cgacagggct aacgcatgcc cgtatggatg
     7441 gacacaccga caactatggt gaagacagtt acaacgaagc cttatcattg aaacgggcga
     7501 atgtcgtggc cgatgcatgg gctatgggtg gacaaattcc gcgcagcaat ctcaccacac
     7561 agggtttagg aaaaaaatat cccatagcca gtaacaagac cgcccagggc cgcgccgaga
     7621 accgccgcgt cgcagtggtg attactaccc cttaaaacac gagcaaacag ataaaaaaag
     7681 ggccagccaa ttggccagcc cttcttaaca ggatgtcgct taagcgaaat cttagttaag
     7741 acgctctttg atacgagcag ccttaccagt acgctcacgc aggtagtaca gtttagcttt
     7801 acgaacagca ccacgacgtt tgacagaaat gctgtcaact accggagagt gagtctggaa
     7861 gacacgctca acgccttcgc cgttggaaat tttacgaaca gtgaatgcag agtgcagacc
     7921 gcggttacga atagcgataa ccacgccctc gaatgcctgc agacgttttt tggaaccttc
     7981 aacaacccat actttcactt ccacggtatc acccggacgg aaggaaggta cgtcctgctt
     8041 catctgctct tgttcaagtt gcttaataat gttgctcata atttaatctc ttatcctggg
     8101 taaactgata tctcgggggc ttacgccatc ccatcatgtt tatgttgctg ttgtgcgtgt
     8161 tccgttttga actccgccag caaccttgct tgctcttcag tcagagccag gttttccaga
     8221 agttcaggtc ttctaagcca ggtacggccc agcgactgtt tcaaacgcca gcgacgtatc
     8281 tcggcatggt tgcccgacag taacactggc ggaacttcca tcccttctaa cacctcaggc
     8341 cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg aatcttccgt tgccgaggct
     8401 tcatgtccca gtactcccgg aataaaccgg gaaacggagt caatcagcgt cattgctggt
     8461 aactcaccac cactgagaac gtaatcgccg attgaccatt cttcgtcaat ttcggtttgg
     8521 atcacgcgct catctatacc ttcgtagcga ccgcacacca gaatcaattt ttgattcgtt
     8581 gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct gtggtgacag ataaatcacc
     8641 tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg cgtcccgcaa gggttgcacc
     8701 atcattaaca tccccggtcc gccgccgtaa ggacgatcgt ccacggtacg gtgccggtca
     8761 tgcgtgaagt cgcgaggact ccagctctgg atgctcagca ggccattttt aactgcccgg
     8821 ccagttaccc cgtaatcggt aattgcgcgg aacatttcag gaaacaggct aattatgcca
     8881 atccacatag cgccgtcttt taccgtttat ccggtggttt aaaaaccagg atcccaatct
     8941 acttcgattg aacgagtagt gagatcgact ttcttgataa cctgcccatc gaggaacggt
     9001 acgagacgtt ccttgatacc aaacgcatct ttcaggtttg ccttaatgac gagaacgtca
     9061 ttagatccgg tttccatcat atcgacgact ttaccgagat cgtagccttc agtggttact
     9121 acctggcagc ccatcaagtc tttccagtag tagtcaccct cttcaagctg aggcaactgc
     9181 gatgaatcca cgacaatttc acaattcgtc agcaggttcg ccgcatcacg atcgtcaacg
     9241 cctttcagct tgatgatcat gtcctgattg tggtgcttcc agctttccag ctggacttgc
     9301 tgccactgac ccgccttctg gataaaccag ggctgatagt caaaaatgct ttcggcgtct
     9361 tcggtggaag aaaacactct gagccaccca cgaataccgt aagacgaacc catttttccc
     9421 aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt tgctcatcat gaccaccgtg
     9481 acagattaag ctgctttgtt tacttctttg atcagcgcag caacgcgatc agaaatagtt
     9541 gcgccctggc caacccagtg agcgatgcga tccagatcca ggcgagtgcc ttcttctttt
     9601 tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga agcgaccgtt gcgtgcatta
     9661 cggctgtcag cgacaacaac ctggtagaac ggacgctttt tagcgccgtg acgtgctaaa
     9721 cgaatagtta ccataacatc ctcttgtgtg aataaaacaa ccggacccca tcgaggaacg
     9781 gagtccggtg tcatattaaa agcccgaaaa ttttactgat ttctggagaa aatgcaatca
     9841 gcagttaata actctgtcgc ataaggtcat ttagcgacca ggaaatcccg gcggcatcat
     9901 acccttcatg cttctcatca tcttcgccat tccgcccttc ttcattttct tcatcatgcg
     9961 ctgcatgtcg tcgaactgtt tcagaagacg gttaacgtcc tgcacctgca taccgcaacc
    10021 ggcagcgata cggcgtttac gcgaaccttt gatgatttct ggcttagcgc gctctttcat
    10081 cgtcatcgag ttgatgatgg cttccatacg caccagcact ttatcgtcca tctgcgactt
    10141 gacgttatcc gggatctgcc ccatgcccgg cagtttgccc atcaggctgg ccatgccgcc
    10201 catatttttc atctggcgca gttgctcaag gaagtcattg agatcgaagc cgtcgccttt
    10261 tttcagcttg ctggctaatt tctcagcttg cgcgcggtca actttgcttt cgatatcttc
    10321 gatcagcgac agtacgtcgc ccatgccgag aatacgggaa gcgatgcggt ccggatggaa
    10381 cggctccagc gcctcggttt tctcgccaac accgaggaac ttgatcggtt tgcccgtaat
    10441 gtgacgaata gagagcgccg caccgccacg agcatcgccg tcgactttag tcagtaccac
    10501 accggtcagc ggtaacgctt cattgaatgc ttttgccgta ttggccgcat cctgaccagt
    10561 catggcatca accacaaaca gggtttcaac cggattaatc gacgcatgga cttgtttgat
    10621 ctcgtccatc atcgcttcgt caacgtgcag acgaccggct gtatccacca gcagcacgtc
    10681 gtagaatttc agtttggctt ctttcagcgc cgcgttaacg atatctaccg gcttctgacc
    10741 aacatcagaa gggaagaaat caacgcccac ctgctctgcc agcgtctcaa gctgtttgat
    10801 tgccgccgga cgataaacgt cggcagaaac caccagcact ttcttcttgt gcttctcgcg
    10861 caggaactta ccgagtttac caacgctggt tgttttaccg gcaccttgca ggcccgccat
    10921 cagcacgacc gcaggcggtt gcgcagccag gttcagggtc tggttctctt cgcccatcgc
    10981 cgcaaccagt tcgttacgga caattttgac gaactcctgc cccggcgtca ggctcttatt
    11041 aacttcatga ccaaccgctt tctcttttac gcgattgata aactcacgca ctaccggcag
    11101 agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc agcgtatctt ttacgttgtc
    11161 ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg cgcgacaaac gatcggttaa
    11221 attatcaaac attgtctctc gcctggggtg gaaacggttg gccgcaatcg cgacacatca
    11281 tcagtatttt gccgcagtat aacatgaagg cgtctttgtt gttatgcaac ggttggagca
    11341 gcgttcacct gacgctatac tgcttctctt tcttattgct caaactgtcg acattactat
    11401 gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc agtcttgcgc tgattgttcc
    11461 cggtctgctg caaaaaaacg gcggctggcg gcgcatggct attatttctg cggtcattgc
    11521 gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg cccgacggtg atagcggaca
    11581 aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg atgatctgta cggtaatgac
    11641 cattgtggct tctcgcaatc gtggctggtt gctgctaccc attgtctatg cctttgcgct
    11701 tatcaacctg gcgctggcga ccttcatgcc caatgaatac atcacccatc tggaagcaac
    11761 gcctgggatg ctggtgcaca ttggcttatc gctcttttcc tatgccacgc tgattatcgc
    11821 cgccctgtac gcgctgcaac tggcgtggat tgattaccaa ctgaagaaca agaagctggc
    11881 gtttaaccag gaaatgccgc cattgatgag tatcgagcgt aaaatgttcc acatcacgca
    11941 gattggcgtg gtgctgctaa cgctcacgct ttgcactggc ctgttctaca tgcacaactt
    12001 atttagcatg gaaaatatcg acaaggccgt gctctctatc gtggcgtggt ttgtctatat
    12061 tgtgctgctg tggggacatt atcatgaagg atggcgtgga cgccgcgtcg tctggtttaa
    12121 cgttgcgggt gcagtcattc tgacactggc ctacttcggc agccgaattg tccagcagtt
    12181 aatcagctaa acccagaaaa ggagtttccc ctggaacaca tttctactac tacgttgatc
    12241 attattctga tcatcatggt ggtcatttca gcctattttt ccgggtccga aaccggaatg
    12301 atgaccctca accgctatcg tctgcgacat atggcgaaac agggtaatcg ctcggccaaa
    12361 cgcgtcgaaa aattgctgcg taagccagac cgcctgatta gcctggtgtt aatcggcaat
    12421 aacctggtca atattcttgc ctccgcgcta ggcactattg ttgggatgcg tttgtacggc
    12481 gatgcgggcg tggcaattgc gactggtgtg ctgacttttg tcgtgctggt ttttgctgag
    12541 gtattgccga aaaccattgc cgcgctgtac ccggaaaaag tcgcttatcc gagtagtttt
    12601 ctgctggctc cgctgcaaat tttgatgatg ccgctggtct ggttgctgaa tgctatcacc
    12661 cgtatgctga tgcgcatgat gggtatcaaa accgatatcg tggttagcgg ctctttgagc
    12721 aaagaagagt tgcgcactat cgtgcacgaa tcgcgctcac aaatttcccg tcgcaatcag
    12781 gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg ttgatgacat catggtgccg
    12841 cgcagtgaaa ttatcggtat tgatatcaac gatgactgga aatcgattct gcgccaactc
    12901 tcccactcac ctcacgggcg catcgtgctc taccgtgatt cgctggacga cgccatcagt
    12961 atgctgcgtg tgcgtgaagc ctggcgactc atgtcggaga aaaaagagtt caccaaagaa
    13021 accatgctac gcgccgcgga cgagatctat tttgtcccgg aaggtacgcc gctcagcacg
    13081 cagttggtga agtttcagcg caacaaaaag aaagtcggcc tggtcgtcaa cgagtatgga
    13141 gacattcagg ggctggtgac ggttgaagat attctggaag agattgtcgg cgatttcact
    13201 acctcgatgt cgccaacact tgccgaagag gtcacgccgc aaaacgacgg ttcggtgatt
    13261 atcgatggca ccgccaacgt gcgagaaatc aacaaagcct ttaactggca tctacctgaa
    13321 gacgatgccc gcacggttaa tggcgtcatt ctggaagcgc tggaagagat ccctgtcgct
    13381 ggcacccgcg tgcgtattgg cgagtacgat atcgatattc tcgacgtaca ggataatatg
    13441 attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg agagtgtggc ggagtaaaaa
    13501 aacggcccgg cattcgcatg ctgggccgtg atttattacg aaagcagaaa ttaagctttt
    13561 gccttcgcta cagtcaccat cgccgcacga atcgtacgac cgttcagcgt ataacctttc
    13621 tgcataatgc ccagtacgtt acctggcgca acgtcatcag attccaccat tgcgatggcc
    13681 tgatgcacat ttgggtccag tgggacgttg gtttcggcga tcacttcaac gccaaactta
    13741 cgcacaacat ccagcatcga cttcagcgtc agctcaatac cttcaaccat cgcggacata
    13801 tccgggttag ctttatcagc cacttccagc gcacgatcca ggctatcaat caccggcagc
    13861 aattcgttga tgaatttctc cagcgcgaat ttgtgagctt tttcaatatc cagttcagta
    13921 cgacgacgca ggttttccat ttcggctttt acgcgcaaaa tgccgtcacg ttcacgggtc
    13981 tgggcttcag ccagctgagc ttcgagattc gcaatttttt catcgcgcgg atccacctgc
    14041 tcagcagaag cttctggctc aactgcctca atctcttcgt gctgatccat gataatttct
    14101 tccggggctt gcccctcagg cgttttctgt tctttactac tcatgaattt ctccgcgttt
    14161 ttttcgcatt catctcgcta acttcgctta ttatggggat cagtttcagg gtttcaaggg
    14221 aagcactcac attgtcatca atcttcgcaa caaggacctc ggaaaaatga ataatcattt
    14281 caagtgtatt ggcattgtgg gacacccacg gcaccccact gcactgacaa cacatgaaat
    14341 gctctaccgc tggctgtgca caaaaggtta cgaggtcatc gttgagcaac aaatcgctca
    14401 cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg gagattgggc aactggcaga
    14461 tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc gcggcgcgca cactcgcccg
    14521 ttacgatatt aaagttattg gaatcaaccg tggcaacctg ggtttcctga ctgaccttga
    14581 ccccgataac gcccagcaac aattagccga tgtgctggaa ggccactaca tcagcgagaa
    14641 acgttttttg ctggaagcgc aagtttgtca gcaagattgc cagaaacgca tcagcaccgc
    14701 gattaacgaa gtggtacttc atcctggcaa agtggcgcat atgattgagt tcgaagtgta
    14761 tatcgacgag atctttgcgt tttcgcagcg ttccgatgga ctgattattt cgacgccaac
    14821 aggctccacc gcatattccc tctctgcggg cggtcctatt ctgaccccct ctctggatgc
    14881 gattaccctg gtgcccatgt tcccgcatac gttgtcagca cgaccactgg tgataaacag
    14941 cagcagcacg atccgtctgc gtttttcgca tcgccgtaac gacctggaaa tcagttgcga
    15001 cagccagata gcactgccga ttcaggaagg tgaagatgtc ctgattcgtc gctgtgatta
    15061 ccatctgaat ctgattcatc cgaaagatta cagttatttc aatacattaa gcaccaagct
    15121 cggctggtca aaaaaattat tctaatttta cgccagcctc tttactgtat ataaaaccag
    15181 tttatactgt acacaataac agtaatggtt tttcatacag gaaaacaact atgttggcac
    15241 aactgaccat cagcaacttt gctatcgttc gtgagcttga gattgatttt catagcggca
    15301 tgaccgtaat aactggcgag accggcgcgg gtaaatctat tgcaatagat gccctcggcc
    15361 tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac cggcgctgct cgtgccgacc
    15421 tgtgcgcccg tttttctctg aaagatacgc cagcggccct gcgctggctg gaagaaaacc
    15481 agcttgaaga cggtcatgaa tgtttgcttc gtcgcgtaat cagtagcgat ggtcgctcac
    15541 gaggttttat caacggtaca gctgttcctc tgtcacaact tcgcgaactg ggccagttgc
    15601 tgattcagat ccatggtcag cacgcccatc agttactcac caaacccgag caccaaaaat
    15661 tcctgcttga tggctatgcc aatgaaacct ctctgctcca ggaaatgacc gcacgttatc
    15721 agttgtggca tcaaagctgc cgtgacctcg cgcatcatca acagttaagt caggaacgcg
    15781 ccgcccgtgc ggaactgctg caataccaat taaaagaact taacgaattt aatccgcagc
    15841 ccggagagtt tgagcaaatc gacgaagagt acaaacatct ggcgaacagc ggtcaattgc
    15901 tgactaccag ccagaatgca ttggcagtaa tggccgacgg tgaagacgca aacctgcaaa
    15961 gtcagcttta cacggctaaa caactggtga gcgaattgat tggcatggac agcaaactgt
    16021 ccggcgtact tgatatgctg gaagaagcta ccatccagat tgctgaagcc agcgatgaac
    16081 tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg actgtttgaa cttgaacagc
    16141 gcatctcaaa acagatttcg ctggcacgta aacatcacgt cagccctgag gcattgccac
    16201 agtattacca gtcgctactg gaagaacagc agcaactgga cgatcaggcc gactcacaag
    16261 aaacgctcgc gctggcggta acgaaacatc atcagcaggc actggaaacc gcgcgcgcat
    16321 tacaccagca acgccagcag tatgcagaag aacttgcact gctgatcacc gacagtatgc
    16381 atgcgctctc aatgccgcat gggcagttta cgatcgatgt taaatttgac gagcatcacc
    16441 tgggcgctga cggtgccgat cgtattgagt ttcgggtaac caccaaccca ggtcagccaa
    16501 tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc acgcatcgca ttggcaattc
    16561 aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat ttttgatgaa gtggatgtag
    16621 ggattagcgg tccaacagcg gcagttgtcg gcaaactgct gcgtcaactt ggcgaatcaa
    16681 ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg atgtggtcat caacactatt
    16741 ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca catgcaatcc ctggataaaa
    16801 aagcgcggtt acaagagctg gcgcgcctgc ttgggggcag tgaagtcaca cgtaacacac
    16861 tggcgaatgc gaaagaactg cttgcggcgt aaactttttt cctgcttcac ggtcagagta
    16921 aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta aagtgatgaa aggtctatta
    16981 tcatcggcat attacagatg agccacgtac tgctcgggcc cgaaaaggaa tcaaatcact
    17041 atgcgctgta aaacgctgac tgctgcagca gcagtactat tgatgttgac cgcaggctgt
    17101 tccactctgg agcgagtggt ttaccgtcct gacatcaacc aggggaatta tctgaccgct
    17161 aacgacgtat ccaaaatacg cgttggcatg acgcaacaac aagttgcgta cgcattgggt
    17221 acaccgctga tgtccgatcc atttggtacg aatacctggt tctatgtctt ccgccagcaa
    17281 ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga cctttaatag tagcggggtg
    17341 ttgaccaata ttgataataa acctgcgctg agtggtaact aatcataaag ttgctctcaa
    17401 agacgttaaa aaaggtgctc aatgagcacc ttttttctgt ctgttattta ttcgctgatt
    17461 tttctgctcg ttgcctgcgc agctctttcg gatcggcaat gagagggcga taaatctcca
    17521 cccgatcgcc atcatgcaca ctatcgctca gttttgccgg acggctataa atgccgactt
    17581 tgtttttagt taaatcgata tcggtacgca attccagcaa gccactggcg cgaatagctt
    17641 cttcaaccgt cgcaccctcc tgcagcgtca cacgctgcag gtactgcttc tcaggtagcg
    17701 cataagccac ctcaacggca attttacctg gcactgtaaa cctctttcgc acgaaccgta
    17761 aaagcctgga ccatattagc cgccagttct ttaaacacgc ggccaaaggc gagttcaatc
    17821 aacttattgg taaactcaaa atcgagatga aactcgatac ggcacgcctc ctgactcagc
    17881 ggcgtaaact tccatccacc gatcaatttc ttgaacggcc catccaccag attcataaga
    17941 atactttggt tactggtcaa ctggttgcgg gtagtaaacg ttttgctgat cccagcctta
    18001 gagacatcta ccgccgcagt catctgccca ggagtggact ccagaatccg acttccggta
    18061 caacccggca aaaactgagg ataagactga acgtcattca ctaactgata catttgctcc
    18121 gcgctgtagg gtaccagtgc ggtccggcta atctgaggca taacaatttc catcaacaaa
    18181 aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa cgctatcccg gcgctgggta
    18241 acatcgggtt catgctaaga tagagccttg tcccccgcag gattgatatg gggtgttttc
    18301 gatttcagat taccgatgat tcacgacgct tatgacgaag aaaaaagcac ataaacctgg
    18361 ttcagcgacc atcgcgctta acaagcgcgc ccggcacgaa tactttatcg aagaagagtt
    18421 cgaggcggga cttgccctgc aaggctggga ggttaaatcc ctgcgtgcag gaaaagccaa
    18481 tatcagcgac agctacgtcc ttctgcgtga cggagaggca tttctgtttg gcgctaacat
    18541 cacgccaatg gctgtggcct ccacgcatgt ggtgtgcgat cctacccgta cccgcaagtt
    18601 acttctcaac cagcgcgaac tggactcatt gtacggtcgc gtcaatcgag aaggctatac
    18661 cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc aaagtgaaaa tcggcgtcgc
    18721 caaaggtaag aaacagcatg ataaacgttc agatatcaaa gaacgcgaat ggcaggtgga
    18781 taaagcgcgt atcatgaaaa acgcccaccg ttaaacctgc actgcaatta ttgatccgct
    18841 cctcaccgcg cctccctctc cggcggcgcg aatgcacatc ttattggcta tcacatccga
    18901 cccaaatgtt gccatcccat tgcttaatcg aataaaaatc aggctacatg ggtgctaaat
    18961 ctttaacgat aacgccattg aggctggtca tggcactcat gaatctggta tacttaccgt
    19021 tacacattgg ggctgattct ggattcgacg ggatttgcga aacccaaggt gcatgccgag
    19081 gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca aacgacgaaa actacgcttt
    19141 agcagcttaa taacctgctt agagccctct ctccctagcc tccgctctta ggacggggat
    19201 caagagaggt caaacccaaa agagatcgcg tggaagccct gcctggggtt gaagcgttaa
    19261 aacttaatca ggctagtttg ttagtggcgt gtccgtccgc agctggcaag cgaatgtaaa
    19321 gactgactaa gcatgtagta ccgaggacgt aggaatttcg gacgcgggtt caactcccgc
    19381 cagctccacc aatcatgatt ggacggtgta aggacaacac caacaaaaac aggaagttag
    19441 cagtctcagc aagacaccga ccagacggtg aggagacata aaaggatacg caaaggagcc
    19501 gcggctcctg gtgatatgaa agcccacaga tgtgggcttt ttcgttgatg gtcagaacga
    19561 ccagttcaca ccagccaccg cgttccacgg ggattccaca ccggcaccat ggctataccc
    19621 caccccgaga tgcccgctta acgaactgct gaatgaggct ttaatacctg cctggtatat
    19681 tccacgtctg cccgacaaat cattgacaaa attaccgtca ttattcactt tcacccggtt
    19741 atcatcgaca aattctttgc gcacagccgc cttcagccac ggctcaactt ccatgccgtt
    19801 ccccagacgc atgttgtaac tcagcgttgc gcccagttca cgatatatac tacgggtatc
    19861 gactgatttc gattccatgc cattggataa atgatattcg gggttatcag cggtgaaccc
    19921 cgttaacgat gcatacggcg tcaggttcca gttaccatcg gtaaatcgca tcccggtttc
    19981 aatgtgaccg cccagcccgt tgctgcgata actgccgttg gcggctccac cgctgctcat
    20041 tttaccggct acgttacttt caaagcggtt cagcttcacg ataccgtcca gatagaaacc
    20101 actttcatgt tcccagctgg catagccccc cagagaataa ctgcccacac tgccatgccc
    20161 tccacgatca aaaaagatat gtgtatggga ataacccata aaagcgccca gcgtcgcaat
    20221 cccctcagga atatcattac ggctatcgat ccccaccgtc attccggtca gcgtctgctc
    20281 aaacccggca cccgcatcgg tggtgacatt attacgggtg ttatacgtcg tcccccagac
    20341 attattgttg tgtggactcg ctttcattat gttcaaccgc tcgcgaatac tgtttagctc
    20401 agcatcaaag accaacggta atgttgctgc catattgagt acggctgccg tagaaggcgt
    20461 gatgcgtttc tccgggacgg gtgtcggcgt tggatcggga gtcgggtctg gtttcggatc
    20521 cggttttggg tctggttttg gatccggctt cggatttggg ttggggtccg ggttgggttt
    20581 gacatcattg gtcaggttcc agttgctgtt gccatcgctt ttcaggacat actcataggt
    20641 cccaagatca acgaaaccgc cggtattacc cagcgtaaac gaagcatccc ctccccctgt
    20701 tttcaccagc gtcatcgcgt cgtcagactg agggctgacg ccggtatcct gaacaaagat
    20761 tttaaaattg ccagtggcgt tgttgttgac gaccagttga tcgccccggg agcctgcaac
    20821 gttggtatgc aggtagaaat taccgctgcc ggaaagttca ctggtggtca gcgtattgta
    20881 gataccatta gttgtggcag gcgctgctgt ttgtgctgat aaataaacat caccgtcgtt
    20941 gaggagcaaa ctatttactc tatattcgca gttgctggtg cctgcacagg aattattgct
    21001 gttaagccag acactgcccc ggctggcagc cgtcaggtcg gcaagcgtcg tatcgacacc
    21061 atttcccaga gttaatgtcg cgctatcggt aatccggacc gccccttcaa gttttactgg
    21121 cgtaacatta tcccgtggcg tcattaacga caggcttcct gtcgcgccac tgaccgatgc
    21181 atccgccagc gtacctgcgt agacgattgc cgtcccgcca gcaacctgta gatcttctgc
    21241 ccgggccaga acctgaaact catcttttga ccgaccgagg gtatattgcc caccagaatt
    21301 aacctttgtg gcgaagtcct gccccaggtt ttgcattgcc ccaccgttgc caaccgtgga
    21361 atcgcgagct tcggtacctg ctaataccaa taaattaccg ccattttcca gcagtacatt
    21421 ggtcgctaaa tatccggcaa tggaaaaggt gccgtactgg tgcgtaccgc tgatttcaat
    21481 accgttagcc gtgctcgtct ggagagcggc accgctgttc tggacgatat ctgtcgcttt
    21541 gccattatcg ttgactgtca gcgttccgcc ttcattgatc gttgttttta ttgcctctcc
    21601 gttagctgaa actgtttgta ttccgccgtc gttaattgtt gtctcatttg ccacaccctc
    21661 gacgatttgt tcaccgccag tgagcgtcgt gccagtcgca gtggcttttg ttttgacgat
    21721 ctcccgaccg cccgcattga cctgtgtttt ggcggaagtg gtgtctgact ccacggttaa
    21781 cacgccgcca tttgtcagca ggatattgtt cgccgcaccc tgctcgatgc tgaacgcgac
    21841 gccatcagcg cgtgtccctg tgacccgcgt cgccctggtg gttgcaacca acgcgccctg
    21901 actactctgc tgtatccccg ttgcgctgcc tttctcccgc acatcgagtg tgccgccgtc
    21961 attaagcacc gagttttcag ccagaccgcc ctcattaact acctgtgaac cgccattaat
    22021 aatggaacct tccgctgtcc cgtttgccat aatttgttgt aagccagaga cgatatcggt
    22081 attgatcgcc ttaccataat tctgaacggt ttgtgtgccg ccattgatgt gcgttttgtc
    22141 tgttgaccca ccatcaacaa tttgttcacc actttcaata tttgcatcag tggctaaacc
    22201 atataccgtt tgcttgccac ctttgatatt tgctttatca gaagtggcac tggcatatat
    22261 tgtttgggtg cccgcactat tgagtacagt gccaacatct tttccataaa catccatttt
    22321 gccgttggca ttaataatcg tatcaactgc ccgggaacca gtgacgactg ttaatgagcc
    22381 agcgttttcc agcactacat ttttagcttc tgaattcctg atgtagaaag catcaccata
    22441 actgttggtt ccttcgataa gggttccgga agtcgtggaa gcaattaatg cgccgccgga
    22501 ttgttgctca acatgcttag cctcaccacc gtcctgaacc tccagaacgc caccattatt
    22561 aagtctggtg ctatctgttt tagcctcctt ctggacaatc agcttaccgc cagcatcaat
    22621 ggtagtattt ttcgccgagg ttttagccat taccgtcagt tcgccggtat tttccagcac
    22681 aacataatta gcctcacctc cggtaatagt gaagtgagag agcttattgt atccttcgat
    22741 atcagtccct gcgcccgtgt tggcaactaa agcactgccc gtctcctggt taaccccatg
    22801 tgcaataccg ccggtataga caatcagcga gcctccggcg ctaatattgc tgccagttgc
    22861 cgtaccatct ttctcaacaa cctgccggct cccagaggat atatttgtcg tgtcagcttt
    22921 cccgccgctc ttaatatttt gtgttccgct gttgatattg gtgcccgt
//
LOCUS       DAZGAP010000079        22375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGAP010000079 DAZGAP010000000
VERSION     DAZGAP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.079"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHLAM_002590"
     CDS             <1..573
                     /locus_tag="ACHLAM_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7854098.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGSSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQ
                     AALTYRYGNEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            912..1385
                     /locus_tag="ACHLAM_002591"
     CDS             912..1385
                     /locus_tag="ACHLAM_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7854099.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1401..1877
                     /locus_tag="ACHLAM_002592"
     CDS             1401..1877
                     /locus_tag="ACHLAM_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7854100.1"
                     /translation="MQQLSFLPGEMTPGERSLILQALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1946..2167
                     /locus_tag="ACHLAM_002593"
     CDS             1946..2167
                     /locus_tag="ACHLAM_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7854101.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2330..2698
                     /locus_tag="ACHLAM_002594"
     CDS             2330..2698
                     /locus_tag="ACHLAM_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7854102.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            2788..3165
                     /locus_tag="ACHLAM_002595"
     CDS             2788..3165
                     /locus_tag="ACHLAM_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7854103.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTSPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKR"
     gene            3162..3650
                     /locus_tag="ACHLAM_002596"
     CDS             3162..3650
                     /locus_tag="ACHLAM_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7854104.1"
                     /translation="MKLSLTLEADSINVRALNMGRIVVDVDGVELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3670..3867
                     /locus_tag="ACHLAM_002597"
     CDS             3670..3867
                     /locus_tag="ACHLAM_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7854105.1"
                     /translation="MESLTTETALDILIAWLQNNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            3952..4818
                     /locus_tag="ACHLAM_002598"
     CDS             3952..4818
                     /locus_tag="ACHLAM_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7854106.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMQSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHRSKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLHLLSGKPIPDNR
                     GNITINLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            4890..5153
                     /locus_tag="ACHLAM_002599"
     CDS             4890..5153
                     /locus_tag="ACHLAM_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO7854107.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            5150..5476
                     /locus_tag="ACHLAM_002600"
     CDS             5150..5476
                     /locus_tag="ACHLAM_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7854108.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHLAM_002601"
     CDS             complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHLAM_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO7854109.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(6428..7606)
                     /gene="gspL"
                     /locus_tag="ACHLAM_002602"
     CDS             complement(6428..7606)
                     /gene="gspL"
                     /locus_tag="ACHLAM_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7854110.1"
                     /translation="MSSMLEIFIPLCASDPIRWQHRTTDVEHGIWSDVDNEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGPDAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(7603..8580)
                     /gene="gspK"
                     /locus_tag="ACHLAM_002603"
     CDS             complement(7603..8580)
                     /gene="gspK"
                     /locus_tag="ACHLAM_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7854111.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPLFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVAQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(8577..9182)
                     /gene="gspJ"
                     /locus_tag="ACHLAM_002604"
     CDS             complement(8577..9182)
                     /gene="gspJ"
                     /locus_tag="ACHLAM_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7854112.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(9179..9550)
                     /gene="gspI"
                     /locus_tag="ACHLAM_002605"
     CDS             complement(9179..9550)
                     /gene="gspI"
                     /locus_tag="ACHLAM_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7854113.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(9547..10110)
                     /gene="gspH"
                     /locus_tag="ACHLAM_002606"
     CDS             complement(9547..10110)
                     /gene="gspH"
                     /locus_tag="ACHLAM_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7854114.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(10114..10569)
                     /gene="gspG"
                     /locus_tag="ACHLAM_002607"
     CDS             complement(10114..10569)
                     /gene="gspG"
                     /locus_tag="ACHLAM_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7854115.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(10586..11809)
                     /gene="gspF"
                     /locus_tag="ACHLAM_002608"
     CDS             complement(10586..11809)
                     /gene="gspF"
                     /locus_tag="ACHLAM_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7854116.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(11809..13302)
                     /gene="gspE"
                     /locus_tag="ACHLAM_002609"
     CDS             complement(11809..13302)
                     /gene="gspE"
                     /locus_tag="ACHLAM_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7854117.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(13302..15362)
                     /gene="gspD"
                     /locus_tag="ACHLAM_002610"
     CDS             complement(13302..15362)
                     /gene="gspD"
                     /locus_tag="ACHLAM_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO7854118.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDDQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(15392..16351)
                     /gene="gspC"
                     /locus_tag="ACHLAM_002611"
     CDS             complement(15392..16351)
                     /gene="gspC"
                     /locus_tag="ACHLAM_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7854119.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(16369..16779)
                     /gene="gspS2"
                     /locus_tag="ACHLAM_002612"
     CDS             complement(16369..16779)
                     /gene="gspS2"
                     /locus_tag="ACHLAM_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO7854120.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(16845..17654)
                     /gene="pppA"
                     /locus_tag="ACHLAM_002613"
     CDS             complement(16845..17654)
                     /gene="pppA"
                     /locus_tag="ACHLAM_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO7854121.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLKALF"
     gene            complement(17784..22339)
                     /gene="sslE"
                     /locus_tag="ACHLAM_002614"
                     /pseudo
     CDS             complement(17784..22339)
                     /gene="sslE"
                     /locus_tag="ACHLAM_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT         5097 a         6303 c         5660 g         5315 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggttc atccagctac cagatgttac ccggatattt tcgtgccatc
      121 tgcaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtgat tgaaggggct tacgaggtgg tgggcgtttt tgaccggata
      241 gaggagaagc gtgatgccat gcagtcgctg attctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt aatgagcatc agcccgtcac caccgctgat
      361 attctgacac cgcgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 atacaggaga atatgctgaa aggcgggatt tccggccgca gtgcaaaagg aaaacgtatc
      481 cacacccgtg ccattcacag catcgacacc gatattaagc tcaaccgcgc attgtgggta
      541 atggcagaaa cgctgctgga gagcatgcgc tgataaaaca tatccctgaa ggcgtgattc
      601 aggtaccaga atcacccgac gccccggagg cgctgccggg gtgtcgttcc atttctgatg
      661 atgacctgtg cacctggtgt acccgtctgc tttaccgtcc cggagaactc agtctgtgca
      721 gactgagtac aggcaatggc atctggccat ccgtctgtga ccggaacggt tacgcgtacg
      781 gctgtccgga atttcagccg aatatcattc atccctgata ttgccgggtc tcacggtcag
      841 tgatgaacgt gatgttgtca gcccccggcc tttatttcct ctcatttatt cacattacaa
      901 ggatttcaca catgaaaaca ttgtctcaga acaccacctc ttcagcctgt gcaccggaga
      961 ctggcctgca acaactggtt gccaccatag tccctgatga acagcgcatc agcttctggc
     1021 cgcagcattt tagcctcatt ccacagtggg tgaccctgga gccccgtgtc ttcggctgga
     1081 tggaccgttt gtgcgaagac tactgcggtg gtatctggaa tctgtacacc ctgaacaacg
     1141 gcggcgcatt tatggcccct gaaccggatg acgatgatga cgaaacatgg gtgctgttca
     1201 atgtcatgaa cggtaaccgc gctgaaatga gtccggaagc cgccggcatt gcggcctgtc
     1261 tgatgacgta cagtcatcac gcctgtcgta cggagtgtta tgccatgacg gtccactatt
     1321 accggctgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcattg
     1381 actgaatgga ggcagggaca atgcagcagc tttcctttct gcccggagag atgacgcccg
     1441 gcgagcgcag tctcattctg caggccctga aaaccctgga ccgccatctt catgaacccg
     1501 gcgtggcctt cacctccacc cgtgcggcac gggaatggct gattctgaac atggcaggac
     1561 tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagttg attgccggtg
     1621 aaaccctctt cacaggcacc atcaaccgca cagaagtcca tccccgggag gtgattaaac
     1681 gcgccctgta ccacaatgcc gccgccgtgg tgctggcgca caatcacccg tccggtgaag
     1741 tcacccccag taaggcagac cgccttatca ccgaacgtct ggtacaggca ctgggcctgg
     1801 tggatatccg ggtgccggac catctgatag tcggtggcag ccaggttttc tcctttgccg
     1861 aacatggtct gctttaaccc gtcaccgcca caatcacatc acacctgttt tcacttttat
     1921 cttctgtctt cagaggtatc ccattatgaa aattatcacc cgtggtgaag ccatgcgtat
     1981 tcaccaacag catcctgcat cccgtctatt tccgttctgc accggtaagt accgctggca
     2041 cggcagtgct gaggcgtata ccggtcgtga agtgcaggat attcccggtg tgctggccgt
     2101 gtttgctgaa cgccgtaagg acagttttgg cccgtatgtc cggctgatga gcgtcactct
     2161 gaactaatgt tccgtcaccc ggaaaacgag tacgaccaga tgacctcaga cgccgctgtg
     2221 gatgaggccc ttctcatcaa tgaatacatc ttcaccgaag ccggtctcca caccggctga
     2281 cctgtcatcg ttaacccctt cttaaccgat tttctgtgag gactttatcg tgtcagacac
     2341 gcaccatgag acaaattatc ccgacgacta taacaaccgc ccctggtggg ggctgccctg
     2401 caccataaca ccctgttttg gcgcccgtct ggtgcaggag ggtaaccggt tgtattacct
     2461 tgccgaccgc gccggtatca gaggccggtt cagcgacgcg gatgcgtacc atctggacca
     2521 ggcctttccg ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc
     2581 ccgccatcag cataccgtca cgctgtatgc cagaggactg acctgcgaag ccgacaccct
     2641 cggctcctgt ggttacgttt atatggctgt ttatccgacg cccgaaacga aaaagtaaat
     2701 catcagaata accttctgct ccggcctggt gttttcacca cgccactttc ccatttttca
     2761 tctctgcata tcaggaaaat cttcagtatg aacacattac ccgacacaca cgtacgggag
     2821 gcatcgcgct gcccatctcc agtaaccatc tggcagacac tgctcacccg actgctggac
     2881 cagcactacg gcctcacgct aaatgacaca ccgttctccg atgaacgtgt gattgagcag
     2941 catattgagg caggcatttc actgtgtgac gcggtgaact ttctcgttga aaaatacgcg
     3001 ctggtgcgta ccgaccagcc ccggttcagc actagtcccc gctctcagtt aataaacagt
     3061 attgatatcc tccgggctcg ccgggcgacc ggcctgatga cccgcgacaa ttacagaacg
     3121 gtaaataaca ttacccgggg taagcatccg gaggcgaaac gatgaaactt tccctgacgc
     3181 tggaagccga cagcattaac gtgcgggcgc tgaacatggg gcgcattgtc gttgacgtcg
     3241 atggtgttga gcttgctgaa ctgattaacg tggtctgcga taacggtcac tcccttcgtg
     3301 ttgttgatga atctgaccgg gcctcaacag actgcacgcc accatttgct gccctgaccg
     3361 gcatacgctg cagtaccgcg catatcacgg caaaagacag cgcctggctg tactcgctgt
     3421 cacatcagac gaatgacttc ggcgaatcag aatggattca ttttaccggg tccggttatc
     3481 tgttacgtac tgatgcgtgg tcgtacccgg ttctgcgact taaacgcctg ggcctgtcaa
     3541 aaacgtttcg tcgtctggtc gtaacgctca tccgccgtta tggcgtcagt ctcattcatc
     3601 tggatgccag cgctgaatgt ctgccgggtt tacccacctt caactggtaa ccgggacaaa
     3661 agacacagta tggaatcatt aaccacagaa acggcgctgg atattctgat tgcgtggcta
     3721 cagaacaata tcgactgcga atccggaatt atcttcgaca acgatgagga taaaacggat
     3781 tcggcagcac tgctgccctg cattgaacag gccagggaag atatccgtac actgcgccaa
     3841 ctgcagtttc tgcaacagaa ccggtgagtc ttactcacca gacttcattc cactgacgcc
     3901 agcctgaata cggctggcgt tttcgtttat aaaccaaaag gaaaaacgat gatgtcagat
     3961 atcacagcct cccgtccgga agtggtcacc gggcatacgg acgttatttg ctcaaccagt
     4021 atccgccaca ttctggctgt acgaaagagt acactgctgc aaatcgacac acttatccgg
     4081 caactggctg aaatctcagc aatgacagaa agtattggcg gtaaaaccgc actggactgg
     4141 gcgatgaaac aggattttcg ctgcggctgc tggctgatgg agaaaccgga aaccgcaatg
     4201 aaagccatca cacgcaatct cgatcgcgaa atctggcgtg acctgatgca acgttccggg
     4261 atgcagagtc tgatggatgc gcagacccgg gacacatggt acaggtcact ggaatatgat
     4321 aattttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact gcaccggagt
     4381 aaggatgaag tgtttgagcg gggagtgatc aacgtcttca aagggctgag ctgggattac
     4441 aaaaccaact caccctgtaa atttggcagt aaaattatcg tcaacaatct ggtgaggtgg
     4501 gaccagtggg gatttcatct tatcagtgga atgcaggcag atcgcctggc tgacctggaa
     4561 agaatgttgc atctgctcag cggtaaaccg atccccgaca accgggggaa tatcaccatt
     4621 aatctggatg accacataca gtctgttcag ggtaaagagg actatgaaga cgagatgttc
     4681 agtattaaat actttaagaa aggctcagta cacatcacgt tcaggaaacc agaactggtt
     4741 gacaggctta atgatattat tgccagacac tacccggaag ccctggcaag cacagtgaac
     4801 aaaagccgca aggcataaaa gaggtatttt acaattcgcg aatattaact attattaatt
     4861 gatagcaatc aatcggcgag aaaatcacca tgcctaccag tgtatccctc agtccttact
     4921 ttgaaacgtt tatccgcgaa caaatagaaa gtggtcggta taacaataca agtgaagtca
     4981 ttcgtgccgg acttcgtgct ctggaagaac gggaacagca gataaagctt gaatcactcc
     5041 agagcgccgt taccgcaggc atcaacagcg gtgaaagtaa aagtgctgaa gaggtattcg
     5101 ggcgactgac gcacaaatac aaaaagatgg cagaaggtga acagccaata tgaaactgac
     5161 tgtttcacca ttagcagaac acgatcttga agctataggt gactggatcg ctcaggataa
     5221 tcctgtccgt gcaatcagtt ttaccgaaga gctttaccaa caatgtctgt tgatagccga
     5281 atcgccagta ctctacagag aaagacccga attaggggct gggatcagag gttgttcgta
     5341 tggtcgttac cttctgctgt tcagggtact ggatactgaa gttcgtatcg aacgtatcgt
     5401 tcatggttcc cgggatatca acagggtact tgcagagcca gacgacaaac catcagatca
     5461 tttctggcag ccataaacaa aatctgtgcg ttaacgctcc ctaacacagg tagccaggta
     5521 ttttataaat ctctgtattt tgttgttact tcgattccgc aattcagcat atcgaaccag
     5581 acaaaatgat tacagcaaag cacaaaaaag agggattata gcgtcaaaag aattcgaatt
     5641 gcacatgggg gcctcattgg tggcctcaaa aacaacaaag acaaaacaat cgatacaaaa
     5701 caattggtta tataaccagt ttcattccga gtccgggcac cactattcta aagaacccgc
     5761 tcacaaggcg ggtttttgct tttggatctg acaataacct tcacgaaaca aattagctta
     5821 taaagcctgg gggaattact ctcgccacgt taacgagagt aattttattg atattaatct
     5881 cctgatactt tatccccgtc caaactccag ccgctgcaca ttcaccatcc cagtcttctc
     5941 agcagcactg acatcaattt gtgtcacccg cagcgcatat ttttcatcca gtgcgtttaa
     6001 ccatttcagc aggtcattaa acaccacagg ttctatccag acctgaatat tctccccacg
     6061 ctcggctatc cgcttgatga ccaccgagtg cgcggaagca ctgtcactga tgacccgcga
     6121 tacctgcgca ggcgttgttg tggcagattt tcgcgctgca ataatatccg gcgcggcgtt
     6181 cttcagtcgg gtgttcattg ccaccagctg ctgcaacatc gtctcctgtt gttctatccg
     6241 ttcgctcaac ggctgccaga tgaggacgta atatccgacg ctaaacagga acaccaccgc
     6301 tgccagtaac atgccctttt cacgcggaga acgccccgcc agatgttgtg tcagccagtg
     6361 ttcgccacgg cttaactggc gttcacgcca ttgctgaaaa tagtgaataa atttatcgcg
     6421 taacatgtta tttcctccgc aacgttacgc cgccggaaac cgcatcaccc tctttctgta
     6481 acgcgtcctg ttgcacaaca taatctgccg ccagtgcgct acgcagtttg tcgaagctgg
     6541 caaagttcgc agcccgtagc tggaggtgaa gcgtctggcg tttttgatca aaggtgaaac
     6601 cacgcatttc gatgtcggta agtgacgctg atttcagggt actggcgatc gctgacaatt
     6661 ctgcgagcag ccgggtatcg tcggcctgcg ggcgatattt tttcagcgcc atcgtcacct
     6721 gagagcgtaa attcacaatc cgcttctgct ccgggaacag tgttaagaac tgtttctccg
     6781 cctgggtgcg gctttgcgcc acctgttcgc tgacgctcca taacgtcacg ccccgttcca
     6841 ctgccagcgc aaccagaatc agcaatatcg gcagaatcat cacccgccag cgcgcccact
     6901 gttttcggta gctgacgcgg ggctgccacg gccctgttag caggttccct tccggttcgc
     6961 cataagtggt aatggcgggc agagccgtaa cggtcaggcg ttcagcatct gggccagccc
     7021 atgctgatag ctcttccggt gcaatgccga ccacggttag tgaaagcggt aaatcctgct
     7081 cattgagctg tgcgcggaac atgaccggag ccagcgccag cccggcgctc catccccggc
     7141 attcatcgat gcggcagata acccgttgcg catcgccagc cataaaccca caaggaatgg
     7201 acatccagtc tggcgcgacg gtagcgcggg tgatgccgtt tgcctgcaac cactgcgcaa
     7261 tgttgcgcat atgctgttgg tgaatcacag ctacggttgc cagttgctgg tcgattttca
     7321 acggggcgaa atgcagttca tcgatatcct ggttcagctc ttcttccagt aaggcgggca
     7381 gaatagtcgg tatctgcttg cgggcgacat caggcagttc aacctgccag acgctgatcc
     7441 attcgccggg aatgtagagt cgaatcgcat cagtttgcag ccattgctgg agctgttcgt
     7501 tgtcaacgtc agaccagatg ccgtgctcca cgtcggtggt acggtgctgc caacggatgg
     7561 gatcggaggc gcaaagcggg ataaaaatct caagcatgga actcactcac tttctcctgt
     7621 ctgatgccag agaaccgaaa agtgttgtgg gcccatgcgg acaattaacg agttcatcgt
     7681 cagttcaatc tcattcacgg tgatatctga acgcagccag aagtaattgc tgtccacgct
     7741 caggacggtt tttagctgtt ttttagtacg ctcatcgacg tcagcaagta gcggctgtgc
     7801 aagaaactga tcgacatctt cccagccctt cgccggacgt tgttgtaata acgcccgcgc
     7861 ctgaacaggg cttaaccacg ggtcaaacag cgcctcaaga atcacacttt gcgcgacgtc
     7921 cagggtatta atgttgattt gctggcgggt catcggcagc gcacagacca gcggtttcag
     7981 tttttgataa agtccggcgt ccattccctg caccacgcgc atctcgctga tatcagccag
     8041 cggttgatta gcggcgtaaa acggcaccga acgggcgaga tactcgctgt cttcacggcc
     8101 cagacgcgtc tgcacgctgc ggtcttcgtc aataaactcc cacaggcttt cggctatcag
     8161 ttcggcccga taagcaggca catccaggcg cgtgatcagg gcaatcagtt gttgtaccgc
     8221 gagcggacgc gacgccgtcg tcggctgagc gagagcattc aggttaaagc aggcctgtgc
     8281 gtcacgcaga gtgacggcga tttgcccctg cggcagtggg aaaaaaagag ggccggaagc
     8341 ccacggctgc gccagatgca cgcgcttttc atttttcagg ctcagactga gtgcgctcaa
     8401 cgccaggctt tccgcactgg cgctgtacca cagcgcctgc tggtactcct gctggctgcg
     8461 cgttcgccca agttgttgct gcattcgccc ggaaagcgtg atggtcacca gcatcataac
     8521 cgccagcaat accagcacca cgaccagtgc cattccgcgt tttggtggtg aggtgatcat
     8581 gataattgcg gcccgcgtaa caaccagatg cgttcaatct cgccccattg cggcgaatgc
     8641 agagtcatgc gcaccgccac tggtatcgcc tgcactgatg accagctctc ctgccagcgc
     8701 gtgccgtcgt agaactgcaa acggagcgaa tccgccggga ttaatttttg cgttgttggc
     8761 ttcacgctgc ctgccgcatc ggtcagtggc caggctaacc gttcgagata accaccatga
     8821 atgcggtaac cgacggtgag cagattactg cgcggcagac gcatcaacgg attaaccacg
     8881 ccgccgcgca caaaacgcat cccttcactc tcagacgcca gcacgccagc gcccgccagt
     8941 aacgctggtt cacgctggcc ctgatcgcct cttaccggac gcggcatcat ttgtgtcaga
     9001 tcgtggttca aaaaactcat cgtttgctgc atgaggttta gtttttgatc gtgtccggcg
     9061 acggcgctat tcacgcgtgt aacgccgttt gtcacctgct gcgccatcag tgccagtgag
     9121 gcaaaaatgg caattgccac cagcatttcc agtaacgtga aaccagcgca agtccttctc
     9181 actgttggcc tcccacggcg ctaaaccagg cgcgtcgtga ctgaatcacc gacgaaaagt
     9241 cttcgtgacg gctgacttca atatccactg cctggagcag cgcgttgcct gtactcagcg
     9301 gtgtggttcg ccagtaccag cggcttcctg ccatatcact ctcgccctgg tgcctgaccg
     9361 ctcgttgttc acgggtcatc aggtaaagca gtgcggtctg gttttcagct acccagcccg
     9421 ctaccgtttt ttcttcaagg atttgctgat tactcagcgc gccgctggcg atctgtaaca
     9481 ccgccatggc ggccaacgca aaaatcgcca gcgcgagcat tacttccagc aaggtaaatc
     9541 cacgcttcat ggcatcccct catcacattg attgagggat aacacgccat cgtgcgccag
     9601 ctttaccgcc caacacgcgt tttgtgccga cgcgtaaaaa cgcagtgtaa acggcgtggc
     9661 gggttcaaaa ggcgaaaaac ggatctgcgg tgtcttcttt ttcgcctctt tttgcagctc
     9721 cagttcaata tcgtgcagcg tcaggcgacg acgctgcagc tccagcgcat attccttctg
     9781 ccagatatca cttccgggtt gtaacagcat ctgcacctgt ttcggcaccg taacctgtgc
     9841 cgataaccgg gtcgcagaaa cgggcaacca ctgcccgtga cgacgctgca taaactggta
     9901 acctggtggg tcgattagca caccgagtgt tttcccttcg atcactgccc tgtccttaaa
     9961 ctgcgcaaag cgagtcagaa aagcctgcgc cgcttttttc gcaggcggct ctgaagcggt
    10021 cgcaaacgtc tgtaccacgc ccgcactggc aaggccgata aggaaaatca ccagcatgat
    10081 ttccagaagt gtgaatccgc gttcaggcac tgattactga aactcctgca aattccagtt
    10141 accgatatct gcgccagcgc cctccccgtt ttcctgacca tctgccccca gggtatacac
    10201 atcaaacagt cctttttcac ccgggctgag atactgataa tcattgcccc acggatcttt
    10261 tggcagtcgt ttaatgtatc caccggtacg gtagttgcgg gagtccgcca tattggccgg
    10321 ttgctggatc agcgcctcca gtccctgttc ggtagtcgga taacgcccgt tatccagtcg
    10381 gtacatatcc agcgcattct ccagcgccac gatatcgctg atggcttttt gccgatcggc
    10441 tttctctttg ttgcccagca ggttaggcac caccagactt gccaggacgc caagaataac
    10501 aatcaccacc atcacttcca gcagggtaaa acctgcccgt ggtttttgtg tgcgggataa
    10561 cgaattcatg tgataactcc gtaaattaca ttccaaccat attgttcagt tgcagcatcg
    10621 gttcgaggat ggcgatgacg ataaacagca ccacgcccgc catcatcacc accagcgccg
    10681 gctcaaacag ccctaacgcc agccccacct gggtatcaaa ttctcgttcc tggttaactg
    10741 cggcctgttc aagcatggtt tccagttcac cgctctgttc gccggaggcg atcatgtaca
    10801 gcatcatcgg tgggaacagg cgcaactccg ccagcgcagc gcgcagactg cttccttcgc
    10861 ggacgcgatc tgccgccagc agcagttgtt gttcgacata gcgatttgcc gacacggcgg
    10921 cagcggtctg aatgccttcc agtagcggaa cactgctggc ggtgaggatg cttaacgtgc
    10981 gggaaaaacg cgccgtattc agcccgcgcg caacacgccc cgtcacgggc aaacgcagca
    11041 aggttttatc ccagcgtagg cgcatggcag gatttttgag tagccgttgc cccagcacca
    11101 gaagccccag caatcccgcc agccagtaaa cgccgctggt ctgtaacgtg tcgctcatgg
    11161 cgatgagcgt gcgggtggag gcaggcagcg cgtgtccgag atgatcaaac tgttcgatga
    11221 ttttcggcac cactgccgtc agcaaaatag tgactacgcc cgttgccacc accagcaata
    11281 ccagcggata gagcatggcc tgcaacagtc gtgatttcag gcgctgtcgc tgttcggtgt
    11341 aatccgcaag gcgattgagc accacgtcga gatgcccgga tttttctccg gcagcgacca
    11401 tcgaacaaaa cagggaatca aagacgcggg gatgttcgcg caggctgtcc gacagggtat
    11461 aaccttcctg aatccggctg cgcagcgcca ttccgaggct tttcacatgc agtttttcac
    11521 tttgctcact gaccgcctgt aagcaggttt ccagcggcat tgctgcctgc accagcgttg
    11581 ccagttggcg cgtgaacagc gccaaatctg ccgccgccac gcgacgatgt gcgtgccgcc
    11641 gacgctgcaa cattccccct gtcgaagcat tcagccgggc ttcaatgtgc acggggataa
    11701 gctctttacc acgcaacaac tggcgggcat gacgcgcgga atccgcctca atcattcctt
    11761 tggttttgcg accattacgc tccagcgcct gatagtaaaa cagtgccatt acgcctccat
    11821 agttacccgc agaacttcat cgagagaggt ttctccggcg agcactttct caatgccgtt
    11881 gctgcggatg cccgcagagt gttgccggac ataacgttcc agctccagtt ccccggcctg
    11941 acggtggatc aaatcacgca atgtggcgtc caccacgatc aactcatgga tggcagtacg
    12001 cccgcggaaa cctttgtgat tacaggcggg gcagccctgc ggatggtaca gcgtgacagt
    12061 acgggcgtcg gtaatcccca gcaggcgttt ttcttcgtca gtggcaggcg cgggctggcg
    12121 gcagtcgggg cacagcgtgc ggaccagtcg ctgcgccatc acgcccgtca ggctggaaga
    12181 gagcaggaaa ggctcaacgc ccatatcctg cagacgtgtg atcgccccca ccgccgtgtt
    12241 ggtatgcagc gtggaaagta ccaggtgccc cgtgagcgac gcctgaacag cgatttctgc
    12301 ggtttctgta tcgcggattt caccgaccat caccacatcc gggtcctgac gcaaaatcgc
    12361 gcgcaggcca cgggcaaagg tcatgccgac gcgggtgttg acctgcgtct gaccgatccc
    12421 ttcaatcatg tattcgatgg ggtcttcaac cgtgagaatg ttgcgcgaat ggttgttcag
    12481 ctcctgcaat ccggcgtaca gcgtggtgct tttgccggaa cccgtcggtc ccgtcaccag
    12541 aaagatgccg tgcggtttgt gtaacagctg gcgcaactgc acggtcagtt gctgactcaa
    12601 ccccagacgt tccagcgtca ggcgggcctg gtttttgtcc agcagtcgca gcaccacgcg
    12661 ctcgccccag gcggagggca tggtggagac acgcacgtca atcgcccgac cgcccagcag
    12721 cagcgcaata cgcccatcct gcggtacgcg cttttcggca atatccagcc gcgccatcac
    12781 cttgatacgc gataccagca gcgaggccag tttgcgcccc gggcgcagca tttcatgtaa
    12841 tgtgccgtca acacgaaaac ggatcaccag actcttttca aatgtctcga tgtggatatc
    12901 cgaagcgcct tctttaatcg cctctgccag catggcgttg atcagtttga tgattggcgc
    12961 gtcgtcgtcg ctttccagca gatcttccgt ttccggcagt tcttcggcga gggtaaaaaa
    13021 atcctcagca gaaccgagat cttccatcag ctggcgggcc tcggaagagt cccgctgcca
    13081 gaccgcattc agccgctgtt caaattcggc ttcgtcgatt tgccgcagcg taaagggcgc
    13141 gttcagcccc cgctgcagct cctgcaaaac agagagcgac aacggatgaa cgtggaggat
    13201 ctccagcgac gcttcgcacc atgccaccag gctaaaccga cggctgaaac tgtagggcag
    13261 acgcacggtg ttagcggtgg tttcctgtgc tacaggcacc attaacgcgt tctcccggca
    13321 ttgaggaatg cgcgaacttc cggcggtaag gcctggtttt gcgctggcag taccggctgc
    13381 gcggtgtgcg gcatcaggct taagccttga tcatcgcgat agatctgctc ggcgcgcatg
    13441 tagttatatt tgcgctgcga cacgccgtct gccgccatac cgtcacgcag aatggtcggg
    13501 cggataaaca ccatcaggtt acgtttttct tttttatccg ccgtcgattt aaacaggtta
    13561 ccaatcaacg ggatatcgcc cagcagcggc actttcgcca cgctttctcc cgcctggtcg
    13621 tccatcagac cgccaagcac gatcagttca ccatcgttag ccagcacggt ggtcttcagt
    13681 ttgcgctcac caaacaccac gtcgaggctg gtctgtcctt ccaccttcga cacttcctgc
    13741 tcaatcacca tctgtaccgc gtttccttcg ttaatctgcg gcgtgacttt cagcatgatg
    13801 ccgacttttt tcctctctac cgtattgaaa ggattgctgt tattggagcc aacggtagat
    13861 ccagttaata ccggaacgtc ctggcccacc atgaagaagg cttcctggtt gtccagcgtg
    13921 gtgatgctcg gcgtggagag cacgttcgag ctggagtcgt ttttgaccgc ctgtaccagt
    13981 gccatccagt cgcctttcac cacgccaacc gccgtaccgc taaagccaga aagcagctgg
    14041 gcgagcgtgg agagatcgcc gttggtatcc gggttaatgg tggtggcacc gttttcactg
    14101 atcaccgtgg agcctttctg cggtttcgcc tgagaaatgg ctgcccccag cgtgccgata
    14161 gggatctgcg taccgttagc aaactgcatt aatccggcat ctttcgacgc ccactgcacg
    14221 ccgaaattga tattgctgcc ttcagcaact tccacgatca acgcctcgac gtgcacctga
    14281 gcacggcgaa tatccagttg ttcaatcacg ctttgcagcg actgcatgat gtcctgcggc
    14341 gcagtaacaa tcagggcatt actgtgtttg ctggcggcga tggagacaac ctcacgcccg
    14401 ctaccgacgg tgccttccgc ctcttcttta gccgccgtga gcgtgccgct gacctgcttc
    14461 agcacatcga ccagatcttc cgctttgctg tatttgagat agaaaacctg gctgttgccg
    14521 ctgcgctcca tttctgagtc cagccgacgg atcaggcggc gcattttgtc ccgcgtggcc
    14581 gggtcaccac tgacaatcac actgttggtg cgttcgtcgg cgacaatttg agatttcagc
    14641 gtcgcaggtt ggttctcgcc gctgtttttc gtcaggcttt ccagcacgcg ggcgatttcc
    14701 gaagccgagg cgttatccag cgggatcacc tcttcagtgc gattacccgc gtgatccaca
    14761 cgctggatca cttccgtcag ccgctccacg acggaggcgc gcccggtgag cataatcacg
    14821 ttggagggat cgtaattaac aacgttgcct gagcctgcgc tgtcaatcat ctggcgcaga
    14881 atcggtgcca gctcgcgtac cgacacattg cgtaccggca cgactttggt aaccatttca
    14941 tcgcccgcgt agttgtcgct gccttcaccg accagcggca gcggctcgac tttcgcggcg
    15001 cttgatttca ccaccttcag cacgtcgttt tccatcggta cgaccgcata cccctgtgct
    15061 tccagcaggt taaggaataa ctggtaatac tggcgttcat tgagcggagt catagtgcga
    15121 atactcactt tcccctgaac gcccggcccc ataatgatgg ttttattaag gttagcgccg
    15181 acggtttcga tgaacgattt caggtcagta tctttaaaat tagcggtgaa agtggcttct
    15241 tccgcccaga ccggtgaact acacaatgcc gctgccagca tcagcggcag taaacgcttt
    15301 tttgttttga ggccagttgt cttcttacgc cagatagaca acgtcatatc acgccaaaac
    15361 acgatgaatg attctcctgg attaaatgcg gttagcgcag tgcgatggaa atgtcgtagc
    15421 gcgcaccctt gcgtaaaacc gtaagttgaa tggaatccat tgaaggtaac tgccgcatca
    15481 gagcaatcat tgctcgtgga tcagtgaaat cctgctgatt tagtgcaatg gcgatatcgc
    15541 cttccctgaa accgctggca tcgaacagag aacgatctgc ccccggcttc actgcataac
    15601 cgacaattcc ctccttacgc acaggcgtaa gctggatata gttaaaaatt ttctgcggat
    15661 ctttcgccag tgcctgacgc acggcagccg ggatctcaac tggcgcactg gcagcaggct
    15721 cagcaacagc ttgctttgct tcgtcgctga cggctttttt gctggtgacc gccacagggg
    15781 ggcgttcctc ctctgccaga ctcagacgtt ccattttccc ctgatagcgc agcatcacat
    15841 ggtcgcggtt gatttcctca atcactgcgt tgtgagagcc aagccgttca ccctgcaaat
    15901 agacctgctg tttgccgcct tcttcaatca ccgcgccggg tctggcacca aaggcgatcc
    15961 cacgcagcac cacattaaga cgcgtttctg ccacaggcgc agattcaggt tgttttaccg
    16021 gtgcggcgac gggttgatat ttgccaaacc agttttgttg gctgactaat tgcacgtcat
    16081 ttttatcgaa cggttttgca tccgcacgga gcggtttatt cgccgatgag gaaaccaccg
    16141 tatattccgc agaaaaggag aaatagcgcc agagtgaata cgccattttt gcagaaataa
    16201 taagcagcat gagccagaac atcccgcgca ccatttttcg cagatgctct ttgtctgtat
    16261 taagagattg tctctggcta agaatgtgac gaattttagt cagccattga atgagataaa
    16321 ttcgtgcgtc acgaaaaaca acccgcgcca atttatatct acccgacgtt atgctttgac
    16381 tattccacag gtggtacgat ccagtttccg ctgatactgg ttacctgtac gtgtatcatt
    16441 aatcgttatg ctgtaattaa ttccctcggt gatcattaat ttcaccgttg ggtcagcgca
    16501 catttgtcgt tgatagctgg ttaaaaaggc gtcaggtgtc tgcgtggtct gagtacccga
    16561 ttcgctgata atggtcattt ttaccgtcgt gccactactt tgcgccagca ctaaggtata
    16621 tcccgcagat ttaatcggca ggttttggct gatattttgc gcctgttttt tcgccagtaa
    16681 actggcgttt tcattatggc tggcgcagcc acttaataat cctgtaacgc tcaacaatag
    16741 cgatattaat accctccctg gcatttgttt tatcgacatg gtaaataatc tctaaggtta
    16801 ttaataagag ttaaaatgtc actttgataa tgacgttgtt atcattaaaa caatgccttt
    16861 agataaagtg ttgctatacc gcccagactt aaacacggtc caaaaggcag tgtggttgat
    16921 cctctttttg taataacggc atatatcagg ccgcagcatg aggcgattaa agcaacgttg
    16981 ggcagtgata acgcccccac ccagccacct aaagcggcga agagtaatac atcgcccatg
    17041 cctaatgctt ctttacgcag aactattccg gctatccagc gcagggagta aaaagtgata
    17101 aatcccacca gtacgccggt gactgcgtct tgtagcgtga gcggactctg ctgcgcccat
    17161 gccgcaatta gtcccgtcca caatacgccc tgagtaaaaa catcgggcag ccattggtta
    17221 tcgaggtcaa tgacgctcgc ggcaatcagc caggcggata atatcatcac cgccagcgcc
    17281 catccacttt ccggccacac caggctcgcc agcaaaaaag cgagtgccgt caataactcc
    17341 accagcggat aacgcttgct gattttcgcc tgacagtcgc ggcagcgccc tttgagcatc
    17401 aaccaggaga gcagcggaat attgtcacga acgcggatag tctgctggca atgcggacag
    17461 tgcgagcgtg gcagcgccag gcttattttt gactgcgtac tcggcatttc accgtgaaac
    17521 tccgccattt gttggcgcag catgatgggg taacgccaaa tcaccacatt caaaaaactg
    17581 ccgataatca atcctccgac ggtagccagg acgggcatcg ccgcggggta ttgctgaaaa
    17641 acatcaaaaa acatggttaa aggttatttg ttgtaacttg ccggatgcgg gcctgcaggt
    17701 gtatgccata cggctttcct ccaggcccga tgcgccttat ttcatgccgg atgcggcgcg
    17761 agcgccttat ccggtataca ggcttactca gctgacatct tatgctcggt aacctgatta
    17821 atggtttccg gcccttgctg cggtttcggc agattaagcg ccgccagcgt ctcgtaagcc
    17881 gactggctca caccgccctc gaagttcatc tcgctcgctc ccggcaactg gtaagcattc
    17941 gcgcccggat tccatttctt aaagaactcc gaaagatccg tctgggcgac ccaggaggca
    18001 cacagcatca gcgtgtccgc tgcgttaccg ttggattcag cacagtagtt cttactgccg
    18061 aacttgttat tgccaacctc atcgccgcgc gctttacggt gcatcaactg gaacaggttc
    18121 cagcctttca tcccttcacg ctcgctgtaa aacttaggca ggtcaccttc tgggtaccac
    18181 tgtttgatat caaagttttt ctctgcccac tctttcagct gtgcgtacat cagcagacgg
    18241 tcccccgcac cgccgcgcgc ccaggcctga ccgttgctct cgtccagata ttccggcgcg
    18301 acggtaatgt cgtcagcgac acggttcatc ttgccgagat agcgatcctg catgtacagc
    18361 gccagcacgt tgttcgccac ttcagttgca cccggtacgt tcagcggtgt ttctgcagcg
    18421 ttatgaccgg cttcgtgcca aatcagccag tcgttcagcg gcgttgtcgg cagcgtggtg
    18481 ctgttcgtcg agaagctgct gttcattacc ggataacccg agtgcgcatc accgatggag
    18541 atctgcacat cgttggcgaa acggtgttta tgccccgtca atgcttcata ggtaaacatc
    18601 cggtggttac cgctcgtttc attgcgaccg tagaagtcat tcatcgagct ggcaaaggta
    18661 tccagatctt tagcgaattc tgctacacca ccagtgaaat tgctggcctc aaggttcttc
    18721 ttcggcgtgg tatagacgaa cgacgcagac tccagctcgc ccagcggcgc cggtgagtcc
    18781 agatcgtttt tccattcgcc gtctttatag aacggcgctt ttactacacc ggtaaaggtg
    18841 aagttagcag aaacatcatc cttactgtcg cccttgatat aaatcagacc accataaggc
    18901 accttgaagg tcacttcacc gttagcctcc agagtatacg ttttagtcac tcttggcgga
    18961 cggttcagcg caacttcatg cttctcacgt ccagtcaggt cgtcagccag cgccacggta
    19021 acagtcactg ggactgacgc cgaagactta atggtgacgt cctgctgggc cggtgcccac
    19081 aggccggttg actgcatgtt acccgcaaac catttggtcg gattcgagta caggctgatg
    19141 ttttccgtaa cgctctcacc ctttgccgat acggatcctg ggtacttctc cacatcaacc
    19201 ttaatgttca gatcccacca ggaacggccc agcatcagac gcgtcagcgg tttttccata
    19261 tagttgagcg gatagcttgg gttcatcatg cccgctttgc tgctaccgtc accgtagatc
    19321 atgttgttat cgaccagcga ttttttcaga tctgcggagc acttggtgcc gcctgcatag
    19381 gcatcattgg cgtagcagtt caggaactcg gtgaacgttt taaagcccag ctcatcttcc
    19441 ttgccctctt cgtaacgata tttcgtatcg ttccacagcc agaccgacat gttctggtac
    19501 agacgttcca gatcaacact gttcagacgc tcacctttac tgcctttggt acggaaataa
    19561 agctcatgct gatacaggcg ctgaatgttg gtgcctaaat ccgccgcctg caccatcgct
    19621 ttcgcggtgt cggcgtcaag attcagttgc gtatagcgcg gaacatacat gccacctgtt
    19681 accggaacat ccgtgcctgg gcggcgctcc aaacagttaa tctcgtaatg gtaagtcgag
    19741 tccttacact cctgtaaccc aggaaacttc tcaaagattt ttgcctttgc cgcttccaga
    19801 gattcttctg ttgtgtattc cgcttcatca ataaaggcat aacgcgttac ctgtttgccc
    19861 tcaacttcct cctgccagct cgcaacttcc agtttcggct tgtcatcagg cttgttgtct
    19921 tgctggtatt tccaggtcac ttcccctgta tttgggtcga tggtgtacgg cggttgcgcg
    19981 ccgtctgcag caggataacg ttcataaacc caaatgccag tcgcgcgacg ctgacgaacg
    20041 cgatccggat acccttgcgg atcgttgttc accaccgatt tgttcagagc cattgacaga
    20101 cccgcggcat ccagcagacg cacaaaactg gacgcgctct cttccttaag attgctcatc
    20161 acgttttcca tgatcagcac cgagccacct ttgttcagat aagcgatcag atcggtcaca
    20221 tcctgctgag tcagcttcgg tttgctggta tctgcacgca gaggcacagc atagggatcg
    20281 ccagaccact gagtcacata ttcaaagccg ttgaggatca gcaacggaat ctcttccgga
    20341 ttcagatcgc catagctggt caactgttta accgtgatac cagtgaaatc ctcatggaaa
    20401 gcaaatggtg cactatttcc caatacctgg cccgcttttt tgaaataaac gttctccagg
    20461 ttggtgccga cagtcatgat gctcttggta tttggctgcc agatgtcatt tgacaagtag
    20521 cgcaggacgt tctgcataaa gtgcttcatg tcatcagaat caccgctgag cgtacactca
    20581 cctttactat taacaccacc gccccaactg taaccgttcg ggcaacgcag gatgctgttg
    20641 tagtgcgggt taccgataac catcagtttg ccttcaccga cttgccccag cgaaataaac
    20701 ggcaggttga aagtcgcagt atcgcgcgta acgttctctg gctgcacaat ggaaggcgct
    20761 tccgtaatgt acgccagctc atttttatcc caggcgcgtt tttcgccaaa cgccagccag
    20821 tagtttttat cattacgcgc catcagaatc gggaatgccg agttggagat atttaccacc
    20881 gcctgaccgc gcgcgttacc ggtgctgcca tagaagttgg tagagtcatg gaagacgtgg
    20941 aacttgctga cagactgata gttcgtatcc acgccccaca gcttgttaat aacgccctga
    21001 atctggccgt cattaacatt gcgcgttgtc agcgagaacc agcgagcctc gttacaaccg
    21061 tcggttttcg cacaaatcgc ggtatcgatc tctttggcct gacccgtctt aaactgctcg
    21121 ataaattcgt taggcagcac aacgttttga tcgccttcat ccagcgtcgc accgttggat
    21181 aacgaaagat tgattatctc gttgatcacg ttgggatatt cggcaaagac cttgcgtaca
    21241 tagtccggaa caacacgagt attattttga ccagtcgtcg aataacgatg aatgagctga
    21301 tcgatatttg ccccgcgaac ttcatcaccc aattcagtca gcgcaatggt cgacttattg
    21361 ccacgtactg agcccagttc aaaggtatcg ataccaaagg agatgtttcg ccccagctaa
    21421 aggagaattt accgttttcg tcagttacgc cacggcctga attggtgtag tagtcaacgc
    21481 cagcaacacc gttccccagg ctatccacca gttggccttc ggaaagaatg atttcagtgg
    21541 gttgatactg ataaaactgt tccgcgttag ccgacacgaa ggacgcgttc agatccggtt
    21601 ttgttccctc tgtcgtgact ggcactaccg ttgaggtatg ggtggacggc gctttatcag
    21661 tcgcagcatt gttttccacc tcttcattga ccagcttgct gaaattgtct gttgccagat
    21721 caatttgctt atacagtttt tcaaatcgcg cgcgatcaac cactgacgag aaagtaagac
    21781 aaagctgttc tgcatctgcg gggcaactgt cgctggacgt caccagagag atggcgttgg
    21841 ttttcttatt ttcagaattc gccagctcct gcgcgtcctc caggctaaac gacactttgt
    21901 caaccgcacg caggctacgc gcagcttctg actgggtgtt gaatgttgca atggtcgtac
    21961 tgcccaccac acaactcacg gtattgcctg gcgtaaaggt aaagccatcg ctggattcac
    22021 cattacaggt agcaccagtt acccgctggc ttccgcccag ggtcagataa cccgttttcg
    22081 taggaacagg ttctggctcc ggttctggct ctggatcagg tgtcggttcc ggcgtaggtt
    22141 ctgggtccgg cgttggctca ggcgtcggct ccgggtttgg tgttggatca ggtttcactt
    22201 ccggcaaaga ccctgttcca gaatctacag gcggcgtatc ggaggaagat ccggaaccac
    22261 cgccatcaca accggctaac agggttgcgc tcaaaatagc cgctaaaagc gatttcttat
    22321 atttaaattt cttattcatt aataacgcaa gtgacaaaac aatgttaaaa aattc
//
LOCUS       DAZGAP010000080        22088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGAP010000080 DAZGAP010000000
VERSION     DAZGAP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.080"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..2397
                     /gene="polB"
                     /locus_tag="ACHLAM_002615"
     CDS             46..2397
                     /gene="polB"
                     /locus_tag="ACHLAM_002615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO7854122.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2561..5467
                     /gene="rapA"
                     /locus_tag="ACHLAM_002616"
     CDS             2561..5467
                     /gene="rapA"
                     /locus_tag="ACHLAM_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO7854123.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5479..6138
                     /gene="rluA"
                     /locus_tag="ACHLAM_002617"
     CDS             5479..6138
                     /gene="rluA"
                     /locus_tag="ACHLAM_002617"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO7854124.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6256..7071)
                     /gene="djlA"
                     /locus_tag="ACHLAM_002618"
     CDS             complement(6256..7071)
                     /gene="djlA"
                     /locus_tag="ACHLAM_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO7854125.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKLQKGFK"
     gene            7326..9680
                     /gene="lptD"
                     /locus_tag="ACHLAM_002619"
     CDS             7326..9680
                     /gene="lptD"
                     /locus_tag="ACHLAM_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO7854126.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            9733..11019
                     /gene="surA"
                     /locus_tag="ACHLAM_002620"
     CDS             9733..11019
                     /gene="surA"
                     /locus_tag="ACHLAM_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO7854127.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11019..12008
                     /gene="pdxA"
                     /locus_tag="ACHLAM_002621"
     CDS             11019..12008
                     /gene="pdxA"
                     /locus_tag="ACHLAM_002621"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO7854128.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12005..12826
                     /gene="rsmA"
                     /locus_tag="ACHLAM_002622"
     CDS             12005..12826
                     /gene="rsmA"
                     /locus_tag="ACHLAM_002622"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO7854129.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            12829..13206
                     /gene="apaG"
                     /locus_tag="ACHLAM_002623"
     CDS             12829..13206
                     /gene="apaG"
                     /locus_tag="ACHLAM_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO7854130.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13213..14055
                     /gene="apaH"
                     /locus_tag="ACHLAM_002624"
     CDS             13213..14055
                     /gene="apaH"
                     /locus_tag="ACHLAM_002624"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO7854131.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14133..14612)
                     /gene="folA"
                     /locus_tag="ACHLAM_002625"
     CDS             complement(14133..14612)
                     /gene="folA"
                     /locus_tag="ACHLAM_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO7854132.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14804..16666)
                     /gene="kefC"
                     /locus_tag="ACHLAM_002626"
     CDS             complement(14804..16666)
                     /gene="kefC"
                     /locus_tag="ACHLAM_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO7854133.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGAVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16659..17189)
                     /gene="kefF"
                     /locus_tag="ACHLAM_002627"
     CDS             complement(16659..17189)
                     /gene="kefF"
                     /locus_tag="ACHLAM_002627"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO7854134.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17297..18628)
                     /gene="yaaU"
                     /locus_tag="ACHLAM_002628"
     CDS             complement(17297..18628)
                     /gene="yaaU"
                     /locus_tag="ACHLAM_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854135.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18687..18974)
                     /gene="fixX"
                     /locus_tag="ACHLAM_002629"
     CDS             complement(18687..18974)
                     /gene="fixX"
                     /locus_tag="ACHLAM_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO7854136.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18971..20257)
                     /gene="fixC"
                     /locus_tag="ACHLAM_002630"
     CDS             complement(18971..20257)
                     /gene="fixC"
                     /locus_tag="ACHLAM_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7854137.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20308..21249)
                     /gene="fixB"
                     /locus_tag="ACHLAM_002631"
     CDS             complement(20308..21249)
                     /gene="fixB"
                     /locus_tag="ACHLAM_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7854138.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21264..22033)
                     /gene="fixA"
                     /locus_tag="ACHLAM_002632"
                     /pseudo
     CDS             complement(21264..22033)
                     /gene="fixA"
                     /locus_tag="ACHLAM_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
BASE COUNT         5301 a         6133 c         5780 g         4874 t
ORIGIN      
        1 gaaaccaggc tataatcaag cctggttttt tattggattt tcagcgtggc gcaggcaggt
       61 tttatcttaa cccgacactg gcgggatacc ccgcaaggaa ccgaagtttc cttttggtta
      121 gcgacggaca acgggccgtt gcaggttacg cttgcgccgc aagagtccgt ggcgtttatt
      181 cccgccgatc aggttccccg cgcccagcat attttgcagg gtgagcaagg ctttcgcctg
      241 acgccgctgg cgttaaagga ttttcatcgc cagccggtgt atggccttta ctgtcgcgcc
      301 catcgccagt tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag
      361 gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc accggtatgg
      421 gtcgagggtg atatacgcaa tggcgctatc gttaatgccc gtctgaaacc gcatcccgac
      481 taccgtccgc cgctcaagtg ggtttctatt gatattgaaa ccacccgcca cggtgagctg
      541 tactgcatcg gcctggaagg ttgcgggcag cgcatcgttt atatgctggg gccagagaat
      601 ggcgacgcct ccgcgctcga ttttgaactg gagtacgtcg ccagccgccc gcagctactg
      661 gaaaaactca acgcctggtt tgcgactcac gatcccgatg tgatcatcgg ctggaacgtg
      721 gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtat tccgctgcgg
      781 ctggggcgcg ataacagcga gctggagtgg cgcgagcacg gctttaaaaa cggtgtgttt
      841 tttgctcagg ttaaaggtcg gctaattatc gacggtatcg aggcactgaa atccgcgttc
      901 tggaatttct cttcattctc gctggaaacc gtcgctcagg agttattagg cgaaggaaaa
      961 tctatcgata acccgtggga tcgaatggac gaaattgacc gccgtttcgc cgaagataag
     1021 cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat cttccacaaa
     1081 actgaaatca tgccgttttt gctcgaacgg gcgacggtga acggcctgcc ggtagatcga
     1141 cacggcggtt cggtggcagc gtttggtcat ctctattttc cccgtatgca tcgcgctggt
     1201 tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg ctacgtgatg
     1261 gattcgcgac ctgggcttta tgattcggtg ctggtgctgg actacaaaag cctgtacccg
     1321 tcgatcatcc gcacctttct gattgatccc gtcgggctgg tggaaggcat ggcgcagcct
     1381 gatccagagc acagcaccga aggctttctc gatgcctggt tctcgcgaga aaaacattgc
     1441 ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg tcagggtaat
     1501 aagccgcttt cgcaggcgct gaagatcatt atgaatgcct tttatggcgt gcttggcacc
     1561 accgcctgcc gcttcttcga tccgcgcctg gcgtcgtcga tcactatgcg tggtcatcag
     1621 atcatgcggc aaaccaaagc gttgattgaa gcgcagggct acgacgtgat ctacggcgat
     1681 accgactcaa cgtttgtctg gttgaaaggc gcacattcgg aagaagaagc ggcgaaaatt
     1741 ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcag aaacgctgca aaaacaacgg
     1801 ctaaccagcg cactggaact ggagtatgaa acccatttct gccgttttct gatgccaacc
     1861 attcgcggag ccgataccgg cagcaaaaag cgctacgccg gactgattca ggagggcgac
     1921 aaacagcgga tggtatttaa agggctggaa accgtgcgca ccgactggac gccgctggcc
     1981 cagcagtttc agcaggagct gtacctgcgt atcttccgca acgagccata tcaggaatac
     2041 gtgcgcgaaa ccatcgacaa gctgatggcg ggcgaactgg atgcgcggct ggtttaccgt
     2101 aaacgccttc gccgtccgct gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc
     2161 gctcgccttg ccgatgaaga aaaccaaaag cgcggtcgcc ccttgcaata tcagaaccgc
     2221 ggctccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca
     2281 ccgctggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactc
     2341 ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct attttgagca
     2401 aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca tccagtacca
     2461 tagcgccctt tccattcctg gacctgaata acaccactac ctcataagca cggtagcggg
     2521 tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta cacttggtca
     2581 acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg cggtggatgc
     2641 gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga
     2701 ttcccccgtg acccgcgtga tgttcaaccc tggtgatact attaccagcc atgacggctg
     2761 gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg
     2821 cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata gcaaactggt
     2881 gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg accgctttgc
     2941 gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt acagcggcct
     3001 gcgcggtcag cgtaccagcc tgatcccgca ccagctcaac atcgctcatg atgttggccg
     3061 ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa ccattgaagc
     3121 cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc tgattatcgt
     3181 cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgctt
     3241 tgctctgttt gatgatgagc gttatgccga agctcagcac gatgcttaca acccgttcga
     3301 taccgaacag ctggtgattt gctcgctgga ttttgctcgt cgtagcaaac agcgtctgga
     3361 acatctctgt gaagccgaat gggatctgct ggtggtcgat gaagcccatc acctggtgtg
     3421 gagcgaagat gcgccgagcc gcgaatatca ggccattgaa caactggcag agcacgtgcc
     3481 gggcgttctg ctgctgaccg caaccccgga acagctgggg atggaaagcc acttcgcccg
     3541 tctgcgtctg ctggacccga accgtttcca cgattttgcg caattcgttg aagagcagaa
     3601 aaattatcgt ccggttgcgg acgccgttgc catgctgctg gcaggtaaca aactgagcaa
     3661 tgatgaactg aacatgctcg gtgagatgat cggcgagcag gatatcgagc cgctgttgca
     3721 agcagcaaac agcgacagcg aagatgccca gagcgcccgt caggaactgg tttcgatgct
     3781 gatggatcgc cacggcacca gccgcgtgct gttccgtaac acgcgtaacg gtgtgaaagg
     3841 cttcccgaaa cgcgagctgc atactattaa gctgccactg ccgacgcagt atcagaccgc
     3901 tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgcg ctcgcgatat
     3961 gctctacccg gagcgtattt atcaggaatt tgaaggcgat aacgccacct ggtggaactt
     4021 cgatccgcgc gttgaatggc tgatgggcta cctgaccagc catcgctctc agaaagtgct
     4081 ggtgatctgc gccaaagctg ccactgcgct gcaactggag caagtactgc gcgaacgtga
     4141 aggtatccgc gctgctgtgt tccacgaagg catgtcgatt atcgaacgtg accgcgctgc
     4201 cgcctggttt gctgaagaag acaccggtgc gcaggtactg ctgtgctcgg aaatcggttc
     4261 tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc cattcaaccc
     4321 ggatctgctg gagcagcgta ttggtcgtct ggatcgtatc ggtcaggcgc acgatattca
     4381 gatccatgtg ccttatctgg aaaaaactgc tcagtcggtg ctggtgcgct ggtatcacga
     4441 aggtctggat gcattcgagc acacctgccc gaccggacgc actatttacg atagtgtata
     4501 caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg acgatctgat
     4561 caaaaactgc cgcgagcaac atgaagcgct gaaagcacag ctggaacaag gtcgtgaccg
     4621 cctgctggaa atccattcca acggtggcga aaaagcccag gcactggcag aaagcattga
     4681 agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg atattatcgg
     4741 tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg atcatatgct
     4801 ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcaccttcg accgtgaagt
     4861 ggcgctggcg cgtgaagatg cacagtttat tacctgggag catccgctga tccgcaacgg
     4921 tctggatctg atcctttctg gcgataccgg tagcagcacg atttcactgt tgaaaaacaa
     4981 agcgttgccg gtaggtacgc tgttggtgga actgatttat gtggttgaag cccaggctcc
     5041 gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc tgctggataa
     5101 aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc agcttaacgc
     5161 ggttaaccgt cacaccggca gcaagctggt taacgccgtg cagcaggatg ttcacgctat
     5221 ccttcagctg ggggaagcgc agatcgagaa atctgcccgt gcattgattg atgccgcacg
     5281 taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagcgc tgcgtgcagt
     5341 taacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc agcaggtaat
     5401 ggaaagcctg gatcaggcag gctggcgtct ggatgccctg cgtttgatcg ttgtgacgca
     5461 tcagtaacgg agccgaagat ggggatggaa aactacaatc cgccgcagga accctggctg
     5521 gttgtcctgt atcaggatga ccatattatg gtggtcaaca agccgagcgg tttgttgtct
     5581 gtgccgggtc gtctggaaga gcacaaagac agcgtgatga cgcgcattca gcgtgattat
     5641 ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat tgtagtggcg
     5701 ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga gcctaaaaaa
     5761 cagtatgtgg cccgcgtctg ggggcatcca tcccctgcgg aaggtctggt ggatctgccg
     5821 ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac aggtaaacct
     5881 gcgcagacgg agtatgaagt ggtggaatat gcggcggata acacggcaag agtggtgtta
     5941 aaaccgatta ccgggcgttc gcatcagctg cgtgtgcata tgctggcgct gggtcatccg
     6001 attctcggcg atcgttttta tgcatcacca gaagcgagag caatggcacc acgtttgttg
     6061 ctacatgcag agatgctgac gattacccat ccggcgtatg gcaatagtat gacgtttaaa
     6121 gcaccagcgg atttttaata gtcacgctgg cgaagttcgg taggccggag aaggcgttca
     6181 cgccgcatcc ggcaattttg cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg
     6241 cctacacgtt gaaacttatt taaacccttt ctgcagctta atcaattcat aggcctgctg
     6301 aatttcctgc gctttttgct tcgccatctc catcatctca ggcggcaaac ctttcgccac
     6361 cagcttatcg ggatggtgtt cactcatcag cttacggtag gcacgtttga tggtggtcgc
     6421 atcatccgtc ggcttcacgc ccagcacatt acaggcatct tccagcgttg ggccacgctg
     6481 cgcttgctgc cagttaccac cgccagattg ctgctgataa ccgccgccaa actgtgcacc
     6541 gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg gagatcccta attcttcagc
     6601 aatgacatac agcacagccc gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg
     6661 aatctggatc tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa
     6721 ctggcgcatc ttttcgcgca gcgggtaatt gtctgatttt cccacccgga acgcgttttg
     6781 cgccgcagta cgggaagcgc catgaaggtt cattcggtcc atcaactggc tggcgatatg
     6841 aatatcagcc tccgtgacgc gacctttgga tttggttaaa tgccccatca cttcaaaagt
     6901 ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg aaccacgcca ttttacggct
     6961 acgggcttta tcaaacatat ggccaattaa caggcctaac actacgcccc aaaagccgcc
     7021 gcccatcagt aaggccacgg ccacgccaat gatttttccc caatactgca tatattcccc
     7081 aaatcgacac acggatatca gggctatctc ccacaatata aaggtgcttt taccgttttc
     7141 cggctgcggt caattgtggg acatcgccta taatttgcat tatcatacct gtcattcaat
     7201 gccgtgccta acaccactga cgctattcgg acaggattaa cactagcgta gagatgacga
     7261 gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa caataaaatc
     7321 aacgtatgaa aaaacgtatc cccactctcc tggccaccat gattgccacc gccctttata
     7381 gtcaacaggg actggcagcc gacctcgcct cacagtgcat gttgggcgtg ccaagctatg
     7441 accgtcctct ggtacagggc gataccaatg acttacccgt gactatcaat gctgaccacg
     7501 cgaaagggga ctacccggat gacgccgtgt ttactggcag cgtggatatc atgcagggta
     7561 acagccgtct gcaggccgac gaagtgcagc tccatcaaaa agaggcgcca ggacaaccag
     7621 agccggtacg taccgttgat gcgctcggta atgtccatta cgacgataac caggtgatcc
     7681 tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga taccaacgtc tgggagggtg
     7741 attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga cctgatgaaa caacgtggcg
     7801 aaaaccgcta taccattctg gataacggta gctttacctc ctgtctgccg ggttctgaca
     7861 cctggagcgt ggtaggcagc gaaattattc atgaccgcga agaacaagtt gcggagatct
     7921 ggaacgcccg ctttaaggtg ggtccggtac cgatctttta tagcccctat ttgcagttgc
     7981 cggtgggtga caaacgtcgc tctggtttct tgatcccgaa cgccaagtac accaccacca
     8041 actactttga gttctacctg ccatattact ggaacatcgc gccaaatatg gatgccacca
     8101 tcacgccgca ttatatgcat cgtcgtggca atatcatgtg ggagaacgaa ttccgctacc
     8161 tctcccaggc gggcgctggc ttgatggaac tggactatct gccttcagat aaagtctatg
     8221 aagatgaaca cccgaacgat gacagttcac gtcgttggtt attctactgg aaccactccg
     8281 gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac caaggtcagc gatcctagct
     8341 acttcaatga ttttgataac aagtacggtt ccagtactga cggctacgca acgcaaaaat
     8401 tcagcgttgg ctatgcggtg caaaacttca atgccaccgt ttcaaccaag cagttccagg
     8461 tcttcagcga gcagaacacc agtagctact cggcagagcc gcagttagac gttaactact
     8521 accagaatga tgttggtccg tttgatacac gcatttacgg ccaggcagtg cactttgtta
     8581 acaccagaga cgacatgcct gaagcaaccc gtgttcacct ggaaccgacc atcaatttgc
     8641 cgctctctaa taactggggc agcatcaata ccgaagcgaa gttgctggca acccattatc
     8701 agcaaactaa tcttgactgg tataactcca gaaacacaac caagctggac gaatccgtta
     8761 accgcgtaat gccgcaattc aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa
     8821 tgctggcgcc gggttatacc caaacgctgg aaccgcgcgc gcagtattta tacgtgccgt
     8881 atcgcgatca gagcgacatc tataactacg actcgtctct gctgcaatct gactactctg
     8941 gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct aaccaggtga
     9001 cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt aatatttccg
     9061 ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata acatgggaga
     9121 atgacgacaa aacgggctca ctggtgtggg caggcgatac ttactggcgt atctcagagc
     9181 gttggggatt gcgtggcgga attcagtacg atacacgtct ggataacgta gcgaccagta
     9241 actccagcat tgaataccgt cgggatgaag accgtctggt acagctgaat taccgttacg
     9301 ccagcccgga atatattcag gctacgctgc ctaagtacta ttccactgct gagcaatata
     9361 agaatggtat ttcgcaggta ggtgccgtcg ccagctggcc aattgccgat cgttggtcaa
     9421 ttgttggggc ctactactac gacaccaatg cgaacaagca agccgactct atgttaggtg
     9481 tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt
     9541 gggataacga taaacaacat gcggtatatg acaacgcaat cggctttaac atcgaacttc
     9601 gcggcctgag ctccaactac ggtctgggta cgcaagagat gctgcgttcg aacattctgc
     9661 cgtatcaaaa cactttgtga tttgttgatt taccacgtaa tccgcagtgc ggttaattga
     9721 aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat
     9781 accagtttcg ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc
     9841 gtgctggaaa gcgacgttga tggattaatg caatcggtaa aactgaacgc tgctcaggca
     9901 aggcagcagc ttcctgatga cgcgacgctg cgccaccaaa tcatggaacg tttgatcatg
     9961 gatcaaatca tcctgcagat ggggcagaaa atgggagtga aaatctccga tgagcagctg
    10021 gatcaggcga ttgctaacat cgcgaaacag aacaacatga cgctggatca gatgcgcagc
    10081 cgtctggctt acgatggtct gaactacaac acctatcgta accagatccg caaagagatg
    10141 attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa
    10201 gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg ccagcactga gctgaacctg
    10261 agccacatcc tgatcccgct gccggaaaac ccgacctctg atcaagtgaa cgaagcggaa
    10321 agccaggcgc gtgccattgt cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg
    10381 attgctcatt ctgccgacca gcaggcgctg aacggcggcc agatgggctg gggccgtatt
    10441 caggagttgc cggggatctt cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt
    10501 ggcccgattc gttccggcgt tggcttccat attctgaaag ttaacgacct gcgcggcgaa
    10561 agcaaaaata tctcggtgac cgaagttcat gctcgccata ttctgctgaa accgtcgccg
    10621 atcatgactg acgaacaggc ccgcgtgaaa ctggaacaga ttgctgccga tatcaagagt
    10681 ggtaaaacga cttttgctgc cgcagcgaaa gagttctctc aggatccagg ctctgctaac
    10741 cagggcggtg atctcggttg ggctacacca gatattttcg atccggcctt ccgtgacgcc
    10801 ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc actcttcatt cggctggcat
    10861 ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg acgctgcgca gaaagatcgt
    10921 gcataccgca tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa
    10981 caacgtgcca gcgcctacgt taaaatcctg agcaactaat ggttaaaacc caacgtgttg
    11041 tgatcactcc cggcgagccc gccgggattg gcccggactt agttgtccag cttgcacagc
    11101 gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac tctccttacc gaccgggcag
    11161 cgatgctcgg tttgccgctc accctccgtc cttattcccc caactcccct gcacaaccgc
    11221 aaactgcggg cacattaacg ctacttcctg tcgcgctacg tgaatctgtc actgcggggc
    11281 agttagcggt tgaaaatggg cattacgtag tggagacgct ggcgcgagcg tgtgatggct
    11341 gtctgaacgg tgaatttgct gcgctgatca caggccccgt gcataaaggc gtcattaacg
    11401 acgcaggcat tccgtttacc ggtcataccg agtttttcga agagcgttcg caggcgaaaa
    11461 aagtggtgat gatgctggcg accgaagaac ttcgcgtggc gctggcaacg acgcatttac
    11521 cgctgcgcga tatcgcagat gctatcaccc ctgcgctttt gcacgaagtg attgctattt
    11581 tgcatcacga tttgcggacc aaatttggta ttgccgaacc gcgcattctg gtctgcgggc
    11641 tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga agagatagac accattattc
    11701 cggtgctcga caagctgcgg gcgcagggga tgaaactcaa cgggccgctg cctgccgata
    11761 ccctgtttca gccgaaatat cttgataacg ccgacgcggt gctggcgatg taccacgatc
    11821 agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt acgctgggcc
    11881 tgccctttat tcgcacatca gtggaccacg gtaccgcgct tgaactggcg ggacgtggca
    11941 aagccgatgt cggcagtttt attacggcgc ttaatctcgc catcaaaatg attgttaaca
    12001 cccaatgaat aatcgagtcc accagggcca cttagcccgt aaacgcttcg ggcaaaactt
    12061 tctcaacgat cagttcgtga tcgacagcat tgtttctgcc attaacccgc agaagggcca
    12121 ggcgatggtc gaaatcggcc ccggtctggc ggcattgacc gaaccggtcg gcgaacgtct
    12181 ggataagctc acggtcatcg aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc
    12241 attcttaggc ccgaaactga ctatttatca gcaggatgcg atgaccttta actttggtga
    12301 actggccgag aaaatgggtc agccgctgcg tgttttcggc aacctgcctt ataacatctc
    12361 cacgccgttg atgttccatc tgtttagcta tactgatgcc attgccgaca tgcactttat
    12421 gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg aacagcaagg cgtatggtcg
    12481 attaagcgtc atggcgcaat actattgcaa cgtgatcccg gtgctggaag taccgccgtc
    12541 agcctttaca ccaccaccca aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac
    12601 gatgcctcac ccggttaaag atgttcgcgt gttgagccgc atcaccaccg aagcctttaa
    12661 ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg tttagcgtcg aggtgttaac
    12721 gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca
    12781 gatggcgaac tatctggcgg agaacgcgcc tttgcaggag agttaacgat gatcaattcg
    12841 ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg cacaatcttc acctgataat
    12901 gaacgttacg tttttgctta taccgtaacc atacgcaatc tggggcgagc gccagtgcag
    12961 ttgttggggc gttactggct gatcaccaat ggcaatggcc gtgaaaccga agtccagggc
    13021 gaaggagtgg ttggcgtcca gccacttatc gcacctggcg aagagtacca gtacaccagc
    13081 ggtgcaatca ttgaaacccc gctgggcacc atgcagggtc actacgaaat gatcgatgaa
    13141 aatggcgtcc ctttcagcat cgacattccc gtattccgac tcgccgttcc cacactcatt
    13201 cattaaaaga atatggcgac ataccttatt ggcgacgttc atggttgtta cgatgaactg
    13261 atcgcattgc tgcataaagt agaatttacc cctgggaaag ataccctctg gctgacgggc
    13321 gatctggtcg cgcgcggccc gggttcgctg gatgttctgc gctatgtgaa atccttaggc
    13381 gacagcgtac gtctggtgct gggcaatcac gatctgcatc tgctggcggt atttgccggg
    13441 atcagccgca ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc ggatgccgac
    13501 gagctgctta actggctgcg tcgccagcct ctgctgcaaa tcgacgaaga gaaaaagctg
    13561 gtgatggccc acgccgggat cacgccacag tgggatctac agaccgccaa agagtgcgcg
    13621 cgcgatgtag aagcggtgct gtcgagcgac tcctacccct tcttccttga tgccatgtac
    13681 ggcgatatgc caaataactg gtcaccggaa ttgcgggggc tgggaagact gcggtttatc
    13741 accaacgcct ttacccgtat gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc
    13801 aaagaatcgc cggaagaggc ccctgcccca ctgaaaccgt ggtttgcgat tcctggccct
    13861 gtcgctgaag agtacagcat cgcctttggt cactgggcat cgctggaagg caaaggtacg
    13921 ccggaaggta tttacgcgct ggatactggc tgctgctggg gtggtacatt aacctgcctg
    13981 cgctgggaag ataaacacta ttttgtccag ccgtcgaacc ggcataagga tttgggcgaa
    14041 gcggcggcgt cttaaacaca gcctgataca ggaaggccgg ataagacgcg accggcgtcg
    14101 catccggcac tagccgtaaa ttctatacaa acttaccgcc gctccagaat ctcaaagcaa
    14161 tagctgtgag agttctgcgc atcagcgtcg tggaattcgc tgaataccga ttcccagtca
    14221 tccggctcgt aatccgggaa atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga
    14281 tacagttttt gcgctttggg caggaactgc tcataaacgc gaccgccgcc aatcaccatg
    14341 atttctggta cgtcaccaca cgccgcaatg gcttcatcca ccgacttcac ccacgttacg
    14401 cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg caacggacga
    14461 ccgatggatt cccaggtatg gcggcccata atcacgggtt tatttaaagt gttgcgttta
    14521 aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc gataacacga
    14581 tctaccgcta acgccgcaat cagactgatc attgagattt cccgataaaa aaaattgtcg
    14641 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    14701 ttatattctg ctggcgagtc cacgctctcc cactggactc gccgcattac aatgaaataa
    14761 aaacaattag ttagctgtaa agtgtgattt acgtcactct ttattaggat gagggtttcg
    14821 tttccggttc atccgccatg ttgccggtat gtttaccttc ttcggttccc tgccagccat
    14881 gtcgttgaat taatgacaga tgttcgcggt cctcggtaat gatctcactt aacatcgcgc
    14941 tggtgcgttt atagaccgcc gcgcgggctt tggtgtcgtt ctcaaccatt gccatctctt
    15001 ccaccatctg aatattaaag cggcggaaca catcggcgcg ttctcgcgct tcatacggcc
    15061 ccagacctaa actttccagt gccagacgcc cggttttcag cgcaccttca aaggtttcac
    15121 gctccggctt ttcaacgcct gcctgacgca aacgaatgta gtggtcgaca tcgcgggcgc
    15181 gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg
    15241 tttgcggatc gtcgatagcg ttaatcagca cttccgcttt cgccgctccg gcagattcca
    15301 gtaaatccat ccgcgtggca tcgccataaa acactttcat accaaattta cgcaaggttt
    15361 cgatatggtc cggatcgtga tcgagcacca ccattttcac cccgctggag agcagtaaac
    15421 gtccggtgat ctgcccaaaa cgaccgaatc cggcgatgat tacccgcggc tgttcttcgt
    15481 cgatctcatc ggcttcacgc gcctcttcac tagaagattg ctcaaggcga ttgaggatca
    15541 ccagcagaat cggcgttgcc gccatcgaca gcgccaccgc cagggtcagc gatttcgccc
    15601 actccggctc cagcacattc gccatctgcg ccgcaccaaa tactacaaag gcaaactcac
    15661 taccctgccc taatagcacc gcaaaccagc gacgctgttt atttggcacc tgcaacggcc
    15721 gggcaatcag ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa
    15781 tgcgcaacgg attttcaagc agcgtgccaa agtctatcga catgccaacg ccgatgaaaa
    15841 acagccccag cagcaaacct ttaaatggtt cgatatcgct ttccagcgca tgacggtatt
    15901 ccgagcttgc cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct
    15961 cttccagcag caaaccaaag ccaaacacga ggaataacgc cacggcacta aacacttccc
    16021 gcaagccaga gcgggcaaca aaacgcagtg ccggacgcgt gacatagcgc cccagcaata
    16081 ccaccagcac cagcgcaccc gccactttta acgccgagag agcaaatgcg cccatcgtcg
    16141 tcgaggcact gctcgccgcc agtagcggga tcatcgccac cagcgggatc gccgcgatat
    16201 cctggaacag cagcaccgca aaggcactgc gacccatttg cgtcaccatc agattgcgtt
    16261 cattcatcgc ctgcatggca atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt
    16321 ccgcgacctg ccagcgcagc ccaagcaaca tacagaacag ccccagcagg ccgccgcaaa
    16381 tcaccatctg caatgcgccg ccgccgaaca ccgccgcacg cagcttccac agcctttgtg
    16441 gatcgagttc gaggccgata ataaacagca tcagcaccac gccaatctcg gcaaagtgca
    16501 gaatagattc ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga
    16561 tcaggtagcc aagtaccgct cccagaccga gacgtaccgc aatgggaaca atcagcgccg
    16621 ccgaaccgag ataaatcagc gcctgaataa gcgtatggct atccatgatg tgcctcctgc
    16681 cattccagca gacgttgctt atagtgccgc gcctgccctt cgagggtttc gtcgtcacaa
    16741 ataaaggtgc agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc
    16801 gcctgtagcg gctgtgacag cacatcaaag cccggatgcg caccaatctc aaaatggctt
    16861 tccccaccgc cggtcgtcac cgcccacagt aaatgtttgc catgcagcgc cgtgccgccg
    16921 tgaccgtaag cccagccgtg cgagaagact ttatcgatcc aaagtttaag aagcggagga
    16981 atgctgtacc actgcatcgg gtgctgccag acgatcagat cggcgcgaga cagcgcctcc
    17041 tgctcggcgg caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg
    17101 acgccttcca gcgtccttgc ctgttcaagc atccgtttat tcgcatggga atgatgcgga
    17161 tacggatgcg cataaattat aagaatcata ggtagcccgc gtgattacgc ctttgttttc
    17221 ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa taatattgat tcagaaaaga
    17281 gagaaatctg aacaaattac cccattctct gcccgcggat cgtcatattg ctggtctgcg
    17341 ccagtgacat ccctcgagtc tccggggcaa acgctacgga aatcaacaag ccaaacagcg
    17401 agatacccgc ccccattagc atcgtgttac tgataccgta attattgata aagatcggca
    17461 gtgcccaggt cgaaacaata gtgccaatac ggcttaagga cataatcacg cccacggcag
    17521 aggcgcggat atccgtcggg aagagttcat taggatagag ccactgcaaa ttacccggcc
    17581 cgccagagaa aaaggcatac accgcaaatg ccatcactac cagccagatc cccatatccg
    17641 ggatcagccc caaaaccgcc agcgccagcg tcatcatggc aaagctgcca atcaacaatg
    17701 gacgccgtcc ggcagtgttt agccacagca tcggtggaat acagccgagc ataaagaaca
    17761 ggctaatcac cacgttcccc agtgccgcgt ttttgccaac acccaatccc aacaaaccaa
    17821 cgatttgcgg gccaaaggtg tagatggcga acatcgggat cacctggcag gtccagatgg
    17881 cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt
    17941 gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa cagtttgatc atcatctctt
    18001 cgcactcttt tactcgccct ttgcgtaaca accagcgggg agattcaggc agttcgaatc
    18061 gaccaatcaa aatcaacaaa caggggatcg ctgcgctacc cagcatccag cgccaaccac
    18121 cttccacatc ataaagccag taaccgacca gatcggcgca ggtcgcgccg acataccaca
    18181 tcgcggcaat aaagctgatg gaaaacgccc gctgacgggt actggagaac tcagtgatca
    18241 tcgaagtggc gatgggataa tctgcaccga tgacaatgcc gatgagtacc cgcatcacta
    18301 acagttcgac gggggatgag acaaacatcg tcgccaccga tatcacgccg atggcgatga
    18361 tatcaatgag gaacattttg cgccgtccga ctttatcgga gatatagcca aacagcgatg
    18421 tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa gccaatccag tcagcgtcca
    18481 gtttcagcgc cggagtcagt tgctccagcg ccacgccaat cattaccagt acataaccat
    18541 ccagaaacgg accaccgctt ccccacagca aaatgcggcg gtgaatagag gagaatttaa
    18601 gatcatcaaa gtttctggac ggttgcatgg gttattcctg tttttttgca atgagagtca
    18661 gtacggcatt acgtatcaaa ccaacatcag ccgtaacgga actccacacc aaaggtgccg
    18721 cgcgggtatt cccactgttc cagcgccgac cccagcccca gaatgcgaca ggtgccgcac
    18781 tccagacatc cggcgtaatc gaagcgcaca ctgccgtcat cctgcttctt gtacagacct
    18841 gcggggcacg ctttcaccag cagctccagg gcctgtttat cggcatcggc cttcacaacg
    18901 atgtgcggat gctcttcatc gacattgaat ttattgacgc ccagtttgac gtccacattg
    18961 acgggagaag tcataaaacg gtcactcctt tcatgccatc cttgatcaga ttgatgaagc
    19021 ccactttctt gccgtggcgg aggattttct tgcgcatcag ttccggcgca ctgccgtcaa
    19081 tggtgaacag gtcacgcgcc acgcccaccg ccagttccgg gtagccgcta aacatgcgtg
    19141 ggttatcaag gaacgccggt agtttctggt acatgcgcat atcgcgcagc gggccactct
    19201 caagatgctg acgatattcc gcgagttttt gcttactgaa atcgtcgctt ttcatcgctg
    19261 aaagcacagt ttttgctgct gcttccccgg cggcaatcgc cagatccata ccgcgaatgg
    19321 taaaaccgag gttcatacac attccggcag catcaccggc aatcaatacg ccgtcaccaa
    19381 ccaactccgg cagcatgttg atacctgctt ccggcactac gtgagcggaa tattccacca
    19441 gcttgccgcc cgcgatcagc ggtgcaacgg ccggatgttg tttgaaatct tccagcattt
    19501 gcggcaccga tttttttgcg tcatgcagat gatgcaaacc acaaaccagc cccagcgaca
    19561 gggtgttttc attggtataa agaaagccgc cgcccatcag gccatcagtg ggggaacccg
    19621 caaacaggca agccgcaccc tgattaccct gcaactgaaa acggtcttcg ataaccgact
    19681 tcggtaattc gatcagttcc ttcacgccaa ccgccacatc cgtcggttta acgcgttttg
    19741 ccatccccag cttttcggca aggatggagt tcaccccgtc agcaaggatc accgttttcg
    19801 cttcaattac atcgccatcg gcttctacgc cgacaacttt gccatcgcgc tgtacgaggt
    19861 tatcgacacg aatcccggta attaactgcg cacccgcttc ttcggcctgc tccatcagcc
    19921 aggcatcaaa tttactgcgc aaaacggagt aagaacgctg ggatggtgag gtttcgtcac
    19981 cgttgcagta gtccatagtc atcgcagact tttccgtcat aaacgcgagt ttttcatggg
    20041 tgatcaggcg ttctacgggg gcggagtcag cgaaaccagg aataatgtgt tccagactgt
    20101 gggcatagag acgcccgccg gtgacgttct tggcaccagc ggaattgcct cgctcgataa
    20161 ctaacacttg cgccccttcg cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg
    20221 caccgacgat gatggcgtca aagatatctt cggacataag agctccctgt cagcggcagg
    20281 gccaaaatgc agccctgcca gagtggatca acgcgctaaa gctgcggtca gtgccggaag
    20341 gatcttcacg gcgtcgccaa caatgccgta atccgcgtac tggaagatcg gcgcattttt
    20401 atctttgttg atggcgaaaa tggtttgcga cgcattagcg ccaaccatgt gctggatctg
    20461 cccggagatc cccaccgcca ggtacagttc aggtttcagc atcaggttgg agataccgac
    20521 atagcgttcg tgctccatcc atttttcgtt ttccgccacc ggacgagaac aggccaactc
    20581 cgcacctatc gccttgcaaa gctgttctgc cagcgcaatg ttctctttgc tgccaatacc
    20641 gcgaccaacg ctgaccacca ggcgggcttt gtcgagatcg acgctgttgc tctgacgcgc
    20701 ctgggttgcc gtgcgggtaa tcgccacagc cggagcctgc cactccacgg tgtgcgtttc
    20761 gccagtgcgt gacgcgtctg gctgagccac atcgaacgtg ccgctgctga tggtcagaac
    20821 cgcatacggc gtggcaatgc gttcttcgcc aatcgccaga ccgccgtaaa ccatgtgttt
    20881 cactgtcgct ttaccgtcct gcacgctaac agcgctggca tcgttagaca ccgccgcgtt
    20941 aaggcggtaa cccagtttcg ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac
    21001 cagaccgtct gcgccgtgct ggcgaatagt gtcagccatg acaccggcgt aatcttcgat
    21061 catccgctcg tccggtttgc cgcttaattt ccagacatga ttagcgccga gctggattgc
    21121 ctgtgcgccg tcggcatcat tgaggacgaa cgtgttgatt tgattagcta aagcctgcgc
    21181 accgttcatc agttccggca gacgagaagg ggtatcgctg aatacccaga cttgagaaaa
    21241 cgtgttcata gcatcccctg taattaaatg actttgcgaa gattttcagc aaatgcggcg
    21301 atctgttctt cgccgtcgcc ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg
    21361 gcaacctgtt gttctgacca ggctgcctct gcgttaaaac cgatatccgc cgccgaccat
    21421 acctggactg gctttttcgc cgcgccgaga atggctttca tcgaaggaat ttgtggggag
    21481 ttgatatcag tggaaacagc aacaaccgca ggcagcggaa tgcttaaggt ttctgtttca
    21541 tctccagttc gcgctcaacg gtgagggtat ctgcagtcag ggagataatt ttgctgacgc
    21601 cgttaactgc cggaatatta aggatttcgc ctaccagcaa accaacctgc tgggcataaa
    21661 ggtcggaaga accatcgcca cagaggatca aatcaaagcc tgctttctga gcggctgcag
    21721 ccagtacgct cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa
    21781 tcagttcatc cggaccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt
    21841 taccgcccac acttaaggcc gtcacctgcg cctctgctgc ctgttgcttt agctggcaag
    21901 ccgcttcaat agcgttgaga tcgtattggc ttattttggc atcggctttg ctgaagtcta
    21961 acgaaccatc agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag
    22021 taataatctt cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt
    22081 taattaca
//
LOCUS       DAZGAP010000081        21878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGAP010000081 DAZGAP010000000
VERSION     DAZGAP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.081"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..621)
                     /locus_tag="ACHLAM_002633"
     CDS             complement(<1..621)
                     /locus_tag="ACHLAM_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7854139.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYR"
     gene            complement(824..1225)
                     /gene="nikR"
                     /locus_tag="ACHLAM_002634"
     CDS             complement(824..1225)
                     /gene="nikR"
                     /locus_tag="ACHLAM_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO7854140.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1230..2036)
                     /gene="nikE"
                     /locus_tag="ACHLAM_002635"
     CDS             complement(1230..2036)
                     /gene="nikE"
                     /locus_tag="ACHLAM_002635"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO7854141.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(2033..2797)
                     /gene="nikD"
                     /locus_tag="ACHLAM_002636"
     CDS             complement(2033..2797)
                     /gene="nikD"
                     /locus_tag="ACHLAM_002636"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO7854142.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENTARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2797..3630)
                     /gene="nikC"
                     /locus_tag="ACHLAM_002637"
     CDS             complement(2797..3630)
                     /gene="nikC"
                     /locus_tag="ACHLAM_002637"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO7854143.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3627..4571)
                     /gene="nikB"
                     /locus_tag="ACHLAM_002638"
     CDS             complement(3627..4571)
                     /gene="nikB"
                     /locus_tag="ACHLAM_002638"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO7854144.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4571..6145)
                     /gene="nikA"
                     /locus_tag="ACHLAM_002639"
     CDS             complement(4571..6145)
                     /gene="nikA"
                     /locus_tag="ACHLAM_002639"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7854145.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6256..6843)
                     /gene="acpT"
                     /locus_tag="ACHLAM_002640"
     CDS             complement(6256..6843)
                     /gene="acpT"
                     /locus_tag="ACHLAM_002640"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO7854146.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6898..7947)
                     /gene="yhhT"
                     /locus_tag="ACHLAM_002641"
     CDS             complement(6898..7947)
                     /gene="yhhT"
                     /locus_tag="ACHLAM_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7854147.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8079..9296
                     /gene="yhhS"
                     /locus_tag="ACHLAM_002642"
     CDS             8079..9296
                     /gene="yhhS"
                     /locus_tag="ACHLAM_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854148.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9300..9857)
                     /gene="dcrB"
                     /locus_tag="ACHLAM_002643"
     CDS             complement(9300..9857)
                     /gene="dcrB"
                     /locus_tag="ACHLAM_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO7854149.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(9930..10595)
                     /gene="yhhQ"
                     /locus_tag="ACHLAM_002644"
     CDS             complement(9930..10595)
                     /gene="yhhQ"
                     /locus_tag="ACHLAM_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO7854150.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10817..11062
                     /gene="tusA"
                     /locus_tag="ACHLAM_002645"
     CDS             10817..11062
                     /gene="tusA"
                     /locus_tag="ACHLAM_002645"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO7854151.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(11164..13362)
                     /gene="zntA"
                     /locus_tag="ACHLAM_002646"
     CDS             complement(11164..13362)
                     /gene="zntA"
                     /locus_tag="ACHLAM_002646"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO7854152.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVMLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13436..14062)
                     /gene="yhhN"
                     /locus_tag="ACHLAM_002647"
     CDS             complement(13436..14062)
                     /gene="yhhN"
                     /locus_tag="ACHLAM_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO7854153.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14203..14562
                     /gene="yhhM"
                     /locus_tag="ACHLAM_002648"
     CDS             14203..14562
                     /gene="yhhM"
                     /locus_tag="ACHLAM_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO7854154.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDI
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14565..14834)
                     /gene="yhhL"
                     /locus_tag="ACHLAM_002649"
     CDS             complement(14565..14834)
                     /gene="yhhL"
                     /locus_tag="ACHLAM_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO7854155.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14824..15420)
                     /gene="rsmD"
                     /locus_tag="ACHLAM_002650"
     CDS             complement(14824..15420)
                     /gene="rsmD"
                     /locus_tag="ACHLAM_002650"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO7854156.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15570..17066
                     /gene="ftsY"
                     /locus_tag="ACHLAM_002651"
     CDS             15570..17066
                     /gene="ftsY"
                     /locus_tag="ACHLAM_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO7854157.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPELVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17069..17737
                     /gene="ftsE"
                     /locus_tag="ACHLAM_002652"
     CDS             17069..17737
                     /gene="ftsE"
                     /locus_tag="ACHLAM_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO7854158.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17730..18788
                     /gene="ftsX"
                     /locus_tag="ACHLAM_002653"
     CDS             17730..18788
                     /gene="ftsX"
                     /locus_tag="ACHLAM_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO7854159.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            19033..19887
                     /gene="rpoH"
                     /locus_tag="ACHLAM_002654"
     CDS             19033..19887
                     /gene="rpoH"
                     /locus_tag="ACHLAM_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO7854160.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20159..21262
                     /gene="livJ"
                     /locus_tag="ACHLAM_002655"
     CDS             20159..21262
                     /gene="livJ"
                     /locus_tag="ACHLAM_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO7854161.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQVGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21450..21833)
                     /gene="panM"
                     /locus_tag="ACHLAM_002656"
     CDS             complement(21450..21833)
                     /gene="panM"
                     /locus_tag="ACHLAM_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO7854162.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5185 a         5797 c         6073 g         4823 t
ORIGIN      
        1 ccggtacggc ggcagcggcg caggcagcac ctcatccgcc tccggcaccc gctcacacca
       61 gccgagtacc caccacggcc cctgcggact gttcgtcgcc agataacgat gcggactcag
      121 gcgaagctct tccggcagcg cctgccagag tgcggccagc cggtgacctt catccagttt
      181 cgccacgccg ccgcgcacca gccagagtga ctcgctgcgg ctgtaaccgt cctcaccggg
      241 aaacaggtgc tcaaaataca ggcttctgcc gccgttatca ctgagtatca gtgtgttatc
      301 gcgcagctgt aagcggatat ccgcaggcat tttccagccg gggccgaggc tccccaccgg
      361 cgcgggcgtt tttgtccggt aactgctgta ggtgcgggag agaatgaacg gcagcgggcc
      421 gggcagggca aggtcggttt cgccgggaag gaccttcgca ccgagcagcg gattgaccgg
      481 atgaccggac gtcaccccgc cggggcacac cgaacaggcc acaccggtgg gggcaccaat
      541 acgtaccccg gcagaaccct gaacaatgct accgccatac tgcgtcatgt cgccctgacg
      601 cgccgccggt tttccgctca tcgtaaaatc cttttctatt attttaatga cagtatcttc
      661 atacgaaaaa gcgaagaaaa acttgtggtc acaatgaatt gaagattttt aggaatatat
      721 ttagaacgtt acaaaagaga aggttcggat agggtattta gagaggaatc aggtgtgcta
      781 gtggcccttt gttgatcgtt caccaaagga cccaataata ggcttaatct tccttcggca
      841 agcactgcaa atgcccgtgc cgcacgccgc gctgggcgat aacgtcatcg gcaaaatgct
      901 gcacgtcgcc catatcgcct ttcagcacgg cgatttccag acagtcgtcg tgattgatgt
      961 ggacatgtag cgtcgcgacg gagagatcgt ggtgatggtg ctgggtggag acaatacggc
     1021 tggctaagtc gcgtttttcg tgttcataca cataggagag caccgcgaaa ccttgcgtgc
     1081 cgtgctgctg ggtagcttcc tgcgccaggg cgctacgcag aatatcgcgg atagcttcgg
     1141 aacggttgtt atagccacga cgctggctca ggctgtccag cgtctccagt aaatcgtcat
     1201 caagcgtgat ggtaactcgt tgcatagact taaacctttt ctgtggtgcg acggcgcacg
     1261 gggaatgcgg gtaataccgc gttttgtagc acacgtccgg cgtcagagga aaaggttaat
     1321 ttatctccca ccacctgggt ttcgacgatt tgtccgttgt ccatcaccat cacccgctga
     1381 cagaagcgtt ccaccaggcg taagtcgtgg gtgatgaaca ggcaggcggt gccaaactgt
     1441 tgttgcagct ttttcagcag gcgaatgacg cccgcctgta acacgagatc aaggttagaa
     1501 acggcttcat ccagaatcag tagtttcggt tcgaccgcca gcgcgcgagc caggcagacg
     1561 cgctggagct gaccgccgct taactgcggt gggcgtttgt ctagaacgct gtcatcgaga
     1621 tcgaccgcct tcagcatttc gctggcacgc gccagttgtt cggctttttt cagcgatagc
     1681 aggtggcgca tcggttcacg caggatctcg cgcacggttt ttcgcggatt cacggcgctg
     1741 atggagtcct gaaataccat ctgaatatcg cggcggaacg ctttacgctg ggcgcgattg
     1801 agctttgcca acggttcgcc gcgccagcta atattccctt gtgagggaga ctctaagcct
     1861 accagcagcc gcgcgagagt gctttttccg cagccgctgc gccccagcag agcgacggtt
     1921 tcgccgcttt tcagggtcag ggaaacgtta ttcagtaccg cctgatgctg atgttttccg
     1981 ctaaatccac cgtgcgcata gtgatgtgaa aggtcgcaga cgttaagtaa agtcatgatg
     2041 ccagctccat accgtagagg gcgagatgag cggaaaccag gctgcgcgtc accgtatgtt
     2101 tgggggcgtt aaacagcgtt tctacatcgc cctgttcgac aattttaccg tgtgacatca
     2161 ccgccacgtc atccgccaga cgcgccacaa cgcccatatc atgggtgacc agcagcattc
     2221 ccggtgcttg tttttgcata atgctttcca gcagatcgag gatgcgcgcc tgtgctacca
     2281 cgtcgaggtc ggtggtcggt tcatcggcga tgataaacgg cgattcacac aacaccgcca
     2341 tcgcaatcat catgcgctgc aacatgccgc cgctcatctc gaacgggtac agcttcaaca
     2401 cgcgcgcggt gttttccaac cccaccgctt ctatggcagc ggtaagcgta gcgtcatcgg
     2461 cgggtttccc cagcgccagg caggtttcac gcgcgtgggt gtgcatggtg tgtagcggat
     2521 taaaggcgct gcgcgggttc tgcatgatgg tggcaatttt gatgccgcgc agggcacagg
     2581 gagaaaccgg tttgccatcg gctaaaattt cccccgccgt ctggcgaacg cctgcgggca
     2641 gaatgcccag cgtcgcggcg caggtcagtg atttcccgct gccgctaccg ccgactaacg
     2701 ccagcacacg cccgcgttta agggttaacg atacgccgtg caccagcggt tgtgcggcct
     2761 gtagcgcgat attacgtagt tcaatctgtt gcggcattag tgtgcgtgct ccgtcaccag
     2821 atgaggatcc agatgatcgc gcagtgcgtc acccaccagg ttaaaggcca tcacgctgat
     2881 aaacagcgcc agccccggcc agaacatttg cagcggctgg gtccagatat actgacgcgc
     2941 gtcgttaatc atcacgcccc attcggcggt cggcgcggtc acgccgagac cgaggaagga
     3001 catccccgcg acgtgtagca tcatatgacc gatatccagc gtcgccagca ccagcagcga
     3061 ggggatcacc gcacctgcca gatgatcaat aaacacccgc acatggcccg cgccagaaag
     3121 ccgtgatgcc agcacaaact cgcgctggcg cagtgaaatc accaggctac gcaccatgcg
     3181 tgcataccac gcccagtgcg acagggcgat ggctataatc acattggtca acccggtgcc
     3241 gagcacgccg accataaaga acgacagaat cgaggttggg aaggtcataa acatatcagc
     3301 gacgcgcatg gtggcctgat caacgcgccc gccaatcagc ccggcgctgc cgccaataat
     3361 cagccctaat gtcagcacca acagcaggca ggccattacc gagccgagcg acacgcgggt
     3421 cgccgccatc agccgcgaga aaatatcgcg ccctaaatga tcggtaccca gccagtgctg
     3481 cgcatccggt gaaagcaggc gcgacggcag atcaatcgcc tgggggtcat acggcagcca
     3541 ccactggctg gtgagcgcaa tcagcgccag cagggcgata atgatcagcg ccaggcgtac
     3601 cgaccagcgg gaagagagga aaaagttcac gcgtgcgctc cttcatgacg acgaatgcgc
     3661 gggtccagcg cggcgttgag taaatcaaca atcaaattac agaccacaaa aaccaccacc
     3721 atcatcagcg taaagcactg gatcaccgga tagtcacggt taaaaatcgc cgacaccgca
     3781 tagcgcccga cgcccggcca ggcaaagatg ttttcgataa tcatcgtccc gccaatcagt
     3841 tcgccaatgt gcatccccac ggcggtgatc atcggcagcg aggcattgcg cagaatatgg
     3901 cggcgttcgg tctgtttgtc gttcaggccg cgtagccgag cccaggtgac gtgacgctga
     3961 ccggcgacgt ccagcatact ggcgcgcagc agccgcgcgt taatcgccag cgacataaag
     4021 gcaatggaaa ccgcaggcaa aatgatgtgc tgccagccgc cgtagcccat cgcaggtagc
     4081 cattgcagat ataccgaaaa cgccatcacc agcaaaaacg ccagccagaa gttgggcatc
     4141 gacacgccga gaaacgcgat gaaacgtacg gcgaaatccg gcagacgatc gcgatggcgc
     4201 gccgcccaga taccgagcgg tacggaagtg agcagaatta acaccagcgc ggcacctgca
     4261 agttccagcg tggcgggcag gaagttcagc atatcgtcca gcaccgggcg ttggctggcg
     4321 aatgagatac caaagtcaag atgcagcgcc ttccacaacc aggtaccgta ctggacgtac
     4381 agcggctgat ccagccccag catggtgcgg gtagaggcca gcatctccgg cgtcggcggc
     4441 aggttagata aacgcagata atcgagcgcc gggtcgccgg taccgaggcg cagcatcaga
     4501 aaaatgatca ccgaggcggc gagcaccatc gggatcagca gcagaaagcg gcgtaatacg
     4561 taacgcaaca ttaaggtttc accggtttaa tctgttcgaa cggaatttcg gtggcgatcg
     4621 gcgcgtaggg gatgttaccc agctccggtt ttgataccac catcattgag atgtaactga
     4681 taggcagata aaccgcctcg tcatgcagac gggtcagaat gtcgcgatac agcgcctgac
     4741 gttgcgtttc gtcatgggtc gccagcactt cgccgatctc tttatcaatc agcggcttgt
     4801 cggctaaacc ttgctgtgcc tggaagtcag cgtgcgacgg tacgcgcatt gaactcagga
     4861 aggcgtgtgg atcgtatggc gcgccccagg tgcggtggaa aatcatgcca aaacgaccgt
     4921 cgcgctgacg agcatagata ctgctctctt cttcgccaat cagcgagaca tctgcgccaa
     4981 tctggcgcat atcagcctga atgatttccg ccatcgattt gcttaacgca tcggtgccga
     5041 tgaacgacag ttcaatgcgc agcggctgac catttttctc gcggatgtct ttgcctgcag
     5101 gcagcgtcca accggctttt tccagcaacg ctttcgcttt ctgcggatcg tactggcgcg
     5161 gtttcaggcc gaggttggcg tagggcacag aaggggcaaa cagggtgtcg gcgacctgct
     5221 gggtaccata caacgcgtta tcaatcagcg attttttgtt caccgcgtaa ttaagagctt
     5281 cacgtactgc cagctcgttg gtgggggctt tggcggtatt gagcgccagc atcacggttt
     5341 cgatcggctg tgacagttga gtgtggtaag ccgggttctg gctaaagcgg gcgaaggtat
     5401 cgagcggtaa taacccttcg tttccgtaca gcaggtcgat atcgccagtt tcaaacgcca
     5461 ccgcacgggt agtcgggtcc gggatcacgt taaaggtgat ctttttaatc gctggctttt
     5521 cgccccagta gttttcgtta cggacgaaga catcgtactg attcagtttc gattcctgta
     5581 aaacccacgg cccggtgcca atcggcgctt taattccgtt catggtttcg tggtttttaa
     5641 actgcgaggg tgcgataaaa cggaaaggac ggggcagggc cagttcttgc aggaaaggat
     5701 agtaggcgct tttcagggtg atttgcagct cgtttttact gagtgctttg acatcaacaa
     5761 tctggtttgc cagctccagc caggcgtgac gttgacggtt atcgagcact gcgcggaagt
     5821 tttctgccgc cgcctctgca tcaaacggtt cgccgttgga gaatttcacg tcatcacgca
     5881 gggtgaaggt ccaggtttta ccatcttctg aatgagtcca gctttttgcc agccacggga
     5941 tcaccgaacc gtctgcctga tatttcacca atggttcata aaccatgctc tgggcgaaca
     6001 tctggttagg cgtgtaaagg tgcgggttta gtggcccgac attcaccggc caggcggtgg
     6061 tgatttcatc tggtgcagcg gcatggacga taaaagacgc acaagccagc agcgcaaata
     6121 gagtgcggcg gagtgtggag agcatggtaa ccccaatgga ttaaaataga tggcggaaat
     6181 aagtatgacg attttaagta ttcgtcatac tgattacctg ttctggatca atagatgggc
     6241 agtcgggtgg gcggatcagt taactgaatc gatccattgc acactgtcgg cggtgagcgt
     6301 aaagggggta ggggtgcaga tcgccaggct taaattgtct agctgacaat ggctgactga
     6361 cagtgatgag tgataggtgc tgtctacgct gacgatttgc caggcgctgc cgccacgctg
     6421 tttaacgatg gcttctttgc gcgtccagat gcgccagaac atttccagtt gctgatcagg
     6481 atgcacggcg tccatctcgg catgttcccc aaggctgaat acggcgttcg ccagccagcg
     6541 ccagttggcg cgcgggcgaa tcacttcgat atcgcagcca acttcgcctt catcgctcaa
     6601 cagcagggcg atatcgtcac cgctatggct taagttgaac catagcggcg tttccggcgc
     6661 gaatgccggt ttgccttgtt cgccatagat gatctccggt agcggggaaa gcgtgtgcga
     6721 aagcaatgca cgccccgcca gccagcgttc gcgtcgtgga ccttgcggtg cttgctcgcg
     6781 taaagccggt ggcagtggag ctgcgcttaa ggtcgaaact ttccccagaa ctatccgata
     6841 catatcaggg ccaacgttta atggaaaatg aaagtgcgta tcgtaacact tgtcgcctca
     6901 tcccggtaac cgacttttcg gtctgcctgg ccccagtaaa atcgccagtt tgctaccgcc
     6961 tttggtggtt tccatccaga ttttacacac gctggttaat ggcaccgaaa gtagcatccc
     7021 taccgggccg agcagccatc cccaaattaa caatgaaaga aataccacca tggtggacat
     7081 ccccagacga tggcccatca tccgtggttc taaaatattg ccgatgacca tatggaccac
     7141 taaaaacaat gcgccgacca gaatacattc gtaaatacca ttaaacagca gcacctgaat
     7201 cattggcggt acggcggaaa ttaccgcacc gatattgggc acgtagttga gcaaaaacgc
     7261 cagtaccgcc cacatcagcg caaactgtac gcccataagt tccagcccca gccagacgat
     7321 aacgcctgtc cataaactta gcagtgtttt caatgccaga taatgcgaaa cgcctttaag
     7381 tgcgcggtgt agtccggcga tgtgaatttg tggattattc aacgcgaaac gcattttgta
     7441 agggacgtgg cgcacttcaa acagcataaa aactacggtc atcaccagca aaagcacgct
     7501 cgccattgcc ccggaaagcc cggtcattag cgctgtggtg aaggtaacca ctttttccga
     7561 gtccatccgc tgcagcattc gctccggtga catatgcaaa ttaagaaaag gcaacatctc
     7621 ctgcaattta aaaagtttgc gcgtcagctc cttattaaac ttcggcagca tagagataaa
     7681 ttcgttaaac gatgccgcca gtacgccgac cagcgcagtt agtgcgatca gcatcaccac
     7741 caccacaatc gtaatggcaa cggggcgttg tactccccga cgaataaacc aggtgacgag
     7801 cgggttgagg acgatggcaa aaaacagcgc cagtaacagc tgcacaatga tatctgccgc
     7861 tgcgtgaatg cccgcgagga tcactaccag cgaggccagc ttgagcagaa tgtgcatgcc
     7921 cgttttatcg ggttgagggg tttccattgg ggcttccttg tgactttttg tattaagtgt
     7981 agcgggagtc acgccagcaa tattctgatt cttactgctg attttcacgt cagcacgtgg
     8041 taaaaatgaa acactgttgt aaaaatgtgg tgatcctcat gcccgaaccc gtagccgaac
     8101 ccgcgctaaa cggattgcgc ctgaatttgc gcattgtctc cattgtcatg tttaacttcg
     8161 ccagctacct caccatcggg ttgccgctcg ctgtattacc gggctatgtc catgatgtga
     8221 tgggctttag cgctttttgg gcaggattgg ttatcagcct gcaatatttc gccaccttgc
     8281 tgagccgccc tcatgccgga cgttacgccg atttgctggg acccaaaaag attgtcgtct
     8341 tcggtttatg cggctgcttt ttgagcggtc tgggatatct gacggcagga ttaaccgcca
     8401 gtctgcccgt catcagcctg ttattactgt gcctggggcg cgtcatcctt gggattgggc
     8461 aaagttttgc cggaacggga tcgaccctgt ggggtgttgg cgtggttggc tcgctgcata
     8521 tcgggcgggt gatttcgtgg aacggcattg tcacttacgg ggcgatggcg atgggtgcgc
     8581 cgttaggtgt cgtgttttat cactggggcg gcttgcaggc gttagcgtta atcattatgg
     8641 gcgtggcgct ggtggccatt ttgttggcga tcccgcgtcc gacggtaaaa gccagtaaag
     8701 gcaaaccgct gccgtttcgc gcggtgcttg ggcgcgtctg gctgtacggt atggcgctgg
     8761 cactggcttc cgccggattt ggcgttatcg ccacctttat cacgctgttt tatgacgcta
     8821 aaggttggga cggtgcggct ttcgcgctga cgctgtttag ctgtgcgttt gtcggtacgc
     8881 gtttgttatt ccctaacggc attaaccgta tcggcggctt aaacgtagcg atgatttgct
     8941 ttagcgttga gataatcggc ctgctactgg ttggcgtggc gactatgccg tggatggcga
     9001 aaatcggcgt cttactggcg ggggcagggt tttcgctggt gttcccggca ttgggcgtag
     9061 tggcggtaaa agcggttcca cagcaaaatc agggggcggc gctggcaact tacaccgtat
     9121 ttatggattt atcgcttggc gtgaccggac cactggctgg gctggtgatg agttgggcgg
     9181 gcgtcccggt gatttatctg gcggcggcgg gactggtcgc aatcgcgtta ttactgacgt
     9241 ggcgattaaa aaaacggcct ccggaacacg tccctgaggc cgcctcatca tcttaaaact
     9301 tactgaataa ccagcgtatt aatgatgttt tctgcggtgg tctgcgcttt ttgctgattg
     9361 tcagctggca gcgtaatttg catggtcagc agttgattac ccacgttacc cagaataacg
     9421 gaagagtacg ccgtctggcc tttcgcggag ataatgctgt ctaactgctg cattttgtga
     9481 cctttcagct caatggcttt attggttacc acttgcagct gcggatcgcg gctacgttgc
     9541 tgatcttcca gacgcttcgc caacaccgcc agatcttctt tcggatcatc gcccatgatg
     9601 acgatgactg ctttttgccc agtggcgtcg gaccagacat gcatgttatt ggcctgcgtt
     9661 cccagcttac cgctctggtc ggtcatatcc gctggcagcg agaaacttaa cttgccatca
     9721 agcaggttga cgggattccc ggtagcgtta ctttccgcga ccgacccctg cgccgtagcg
     9781 ttagtgtctt tatcatcaca ggccgcaagc cccataacca gcaggccaat tccgacatat
     9841 ttaaccagat tgcgcattga cttcttcctt tcgataaacg gccataacgg ctcattcatc
     9901 catcttatca caactctgat aacgaacctt taactcgcct gcaaagcgtt gatttcagat
     9961 ttatctgcca gtcttttcaa cagcatattg agtaatacgc catacattgg caggaagaaa
    10021 acgatactga ttaacacttt gaaacagtaa tcgaccagcg cgatttccat ccagtgttca
    10081 gccataaagg catccgggct acgccagaag gcaatgaaga agaaggccag cgtgtcgctg
    10141 acgttaccga acagtgtgga cgctgtcggt gccagccacc agtgacgact ctgacgcagg
    10201 cggttaaaaa cgtgcacgtc gaggatctgc cccagcgcgt aggccatgaa actggcggtg
    10261 gcgatacggg cgacaaacag gttgaagtgg gcgagcgcgc cgaatccctg ccaggaaccc
    10321 atatagaaca gcgacgagat gacgtaggag atcagtaacg ccgggatcat caccgcaaag
    10381 atgatacgac gggccagcgg tgcgccaaaa atacgcacgg tcaggtcggt agcaaggaaa
    10441 ataaacggaa agctaaatgc gccccaggtg gtatggaaac caaaaatgga gacgggaagc
    10501 tgaaccagat agttactgga ggtgatcacc agcagatgaa ataacgataa ccagaacaac
    10561 gccttatagc gttgagtttg cgagaaaacg ttcatattgt acctttttga ttaaccattg
    10621 gggtgaggga acccaatacg tacgacacgc tattttacct tgattaaata acgttcttac
    10681 ccttttttcg agccgccgca tgatactgct ttacgttgac aatgcaatgg ttaattttca
    10741 cgcaatcgtt aacctggttt gcttacggac tcacagggcg taaactagcg ccgttttttt
    10801 atgtgatgag aagaaaatga ccgatctctt ttccagccct gaccacacac tcgacgcact
    10861 tggcctgcgc tgcccggaac cagtaatgat ggtgcgcaaa acagtgcgca atatgcagcc
    10921 aggcgaaacg ttgctgatta tcgccgacga tccggccact acccgcgata ttcctgggtt
    10981 ttgtaccttt atggaacacg aactggttgc taaagagacg gatgaactgc cttatcgtta
    11041 tttgattcgc aaaggcggtt gatggggaaa gattggcttc gatgccgcct tttcccctca
    11101 ccctaaccct ctccccagag gagcgagggg accgatcgcg ctcaatgttg cgatcggttt
    11161 gccttatctc ctgcgcaaca atcttaacgc attcgctgtc accagcaccg tcgcacccgt
    11221 atctgccagc accgccagcc atagcccggt cattcctaac agtgtggtga cgaggaagat
    11281 ccctttcagc cccagcgcaa tagtgatgtt ctggcggata ttggcgtgag tggcgcgtgc
    11341 cagttcaatc atttgcacca ggccgcgcag gtggttatgg gttaatgctg catcggcggt
    11401 ttccagcgcc acgtctgtgc cactgcccat tgcaatcccg atggcggcgg ctttcatcgc
    11461 tggcgcgtcg ttaataccgt caccgaccat cgccagcggc gcatgttgat tcagctcggt
    11521 caccgctttg actttatctt ccggcaacag gcccgcttta aactctagcc ccagttcccc
    11581 ggcgattgcc gccgctgcgc gtggattatc gccggtgagg atcacccctt tgacgcccag
    11641 cgcgttcagt tcactgatgg cagttgcagc atcggcgcgc agggtatcct gtaacgcaat
    11701 gacaccaagc acgtcatcgt tacgtactac cagcactacc gtttgcccgg cgctttccag
    11761 ttcgttaatt aaaccagcaa atgcatcagc gggatgtttc ccggcagcgc atatcaacac
    11821 gcgctcaccg ttaacctgcg cttcaatgcc agacccaacc agcgcccgct gtgattgggc
    11881 ggcgggaatg gcgagttcag caacctgtgc ttctcgcacg atggcttgcg ccagtggatg
    11941 cgtcgcgcct tgctcgaccg ccgctgccag tgtcagcagt tcggattcac taatacccgt
    12001 tgccggatga atcgcggtaa cgcgcggttt accgacggtc agcgtaccgg ttttatcaaa
    12061 cgccacctgg gtaacacgac ccagttgttc cagcgccgct ccgcctttaa ttaacgcccc
    12121 acgacgcgct gctgctgcca gcccggaggt aatcgccgca ggcgttgaga taactaacgc
    12181 acacgggcag ccaatcagca gcagcgtcag ccctttataa atccactctt gccagctggc
    12241 ggcaaacagc agtggtggca ccagcgttac cagcagagcg acggccataa tcgctggcgt
    12301 gtaaatacgg ctgaaacggt cgataaaccg ctcaatggga gcgcgacgct cttcggcttc
    12361 ttcgatcagt ttcagaatcc ggtcaatggc gctggcaccc ggttctgaca gcacttccag
    12421 cattaccaga cggtctacgc tggtggcgcc tgcaggaact ttatcgcccg tcgcgcgctc
    12481 cacaggaatg gattctccgg tcagggcgct ttcatcaaaa ctggcaaacg gtgagagcag
    12541 tttaccgtcg gcaggcaaac gcccacctgc cgcaacttca atcacatcgc caggccgcag
    12601 gctgtttatt gccacctctt cacgctcacc gttacgcaga cgcgtggcgg tttctggctt
    12661 cagcgccatt aacgcgctga ccccttgacg cgcgcgactg gcggcccagc cctccagtcg
    12721 ttcgccaatc aaaaacagca gcaacaccat cgcagcttca gccgttgcgc caataaacag
    12781 cgcaccaata gcggctacgc tcattaacgt ttcaatggcg aagtagctgc cggatttgat
    12841 cagccgtaat gcctgacgag caatcgggta cagcccaacc agcgtggtcg cgataaacgc
    12901 cagttgcccg aacggatgat tgaactgctc cagaccccag ctgattgcca tcatcacgat
    12961 tagcgtaatc agcggcagat tctctttcag gcgtgatgct tgtggttcgt cggcggactg
    13021 ttcatcgcgc agggaatagc ctgctttttg caccgcagat tcaacctggg cacggatgtc
    13081 attgtcggca tcgaccacca gtttttcggt ggcgaacaac acctgcacct gattcacgcc
    13141 tgcaagctgg cgcacggcat tttctacctt gcgcgcacag gcggcgcagt ccatgccgct
    13201 gactttccag ctatagcggg tgccggagac gttttcagag agagttggcg tgctggaaca
    13261 tgcgccgtcg cagcaacagt cgttggcgtt ctgtaccgtg gttagcggtt tgaacgcagc
    13321 aaattgaggg gctttcttgc cgtgattgtc aggagtcgac atggcatcct ccggttaagt
    13381 tttttctcat taaccgaagg atacactctg gagtcgactc cagagtcaag ttttatcaga
    13441 gatacagcga gcggacgatg aggaaatgac cggcaaagta gcaggccgca gcaatcgcgt
    13501 tatctgcccg gaagcggcgg cgatagtggc tacccagcca gacaaagtta ctgataaaca
    13561 gcaacgaagc gccgacaaac gcagagagcg ccggagctgt cggacggaag aaccacagtt
    13621 cacctgccag ccacaccatc accagcgtca tgccgataaa cgtgcagata ggccaacgat
    13681 actcttccag acgcgtccag ataatcgcca gtaacagcgc acccagaacc agcaacacca
    13741 gtggcagagg ccagaagaaa gagagcgtca tctgactggc aaaatagatg gtgtacagca
    13801 ggtgcgagag gaaaaacgcg ccgatggcgt acatcagacg ttgacgtggc aacagggtta
    13861 gcgcatcgcc cagcagtgag gcgcacagcc ctgccagcac cagatagcta atggcgtcga
    13921 acatcggcgc ttgccaggcc agtaacagca ggagaagaag ggttaacggt ttaaacaccc
    13981 agcgttgcca ggttggccca cgatacgatg catccacaga tagccatgcg gaaagacaga
    14041 cagcgataaa cgaccaaagc atcttagttc cttgaattcg ttattttttc gtaagcggaa
    14101 tgcctacggt cttgcttcca gtttagtggc gagggtggac ggatgacaac accataactg
    14161 gcagggtatg cttatttccg cattttccga tgagggataa agatgagcaa accacctctt
    14221 ttctttattg ttatcattgg cttaattgtc gtcgccgcat cgtttcgttt tatgcaacag
    14281 cgacgggaaa aagctgataa tgatatggct ccgctccagc aaaagctggt ggtggtgagc
    14341 aacaagcggg aaaaaccgat taacgatcgc cgttcgcgcc agcaggaagt gactccggca
    14401 ggcaccagta tgcgctacga ggcaagcttc aaaccgcaaa gcggaggaat ggagcagacg
    14461 tttcgcctcg acgctcagca gtaccatgcc ctgacagtgg gcgatatagg tacgctgagc
    14521 tataaaggaa cgcgctttgt tagctttgta ggcgaacaat aaggttattt tttaacttta
    14581 aatttcttct gccagaccag cagttcaaac acgccgaaaa ggaaaatccg caccttttcg
    14641 gcggtggtca tctgcgggcc atctttaggt aaagtggatt tcaatagcgc cagctgcata
    14701 ccatgcatca gcacggtaaa aatcagcgcc acgttaacga aaatattcag cgggcgcggg
    14761 aagggatgca ccagattgag gattaaaaat ccccaaacgc agagcattaa caaacgacca
    14821 atattaatca gcatcacttt ctccttgtgc ttcgcgttga tacagccgat aagccacctg
    14881 acccgccact ttttcccgat gtaatgacca gtttgccgga acagtgggca gaccgttttc
    14941 gacttcgctt tcgacataaa tcagggcttc gtcagccagc cagccgttat cttccagtaa
    15001 atttatcgtc tcttctaaca agccacggcg gaacggtgga tcgacaaaca cgatattatg
    15061 cggtgtgcct ttttgcgcca ggaatgacat tgcgttgctg ttcaccacgc gtgcattgcc
    15121 tgcttttagt gtcgccagat tcttaattaa ctgctgagaa accgcgcgat ccatctcaat
    15181 caacgtggcc cccgcagcat agcgcgacaa cgcttccagc cccagcgcgc cgctcccggc
    15241 gaagcaatcc agacattggg cgtcaacaat gaccggagcc agccagttaa acaacgtttc
    15301 gcgtacgcgg tcggtggtgg ggcgcagacc tgggctatca ggaaccggga gtttacggcc
    15361 tcgccactgc ccgccaataa tgcggatttg gccgctgccg gaatgattcg gttttttcat
    15421 gataaattgc tcaatccgcc agataacaca tacttgcagg cggtgatgtg aattaagtta
    15481 agtgatagac tatttcatca tttttttagc tgctatgtac atagcgttaa cgctgtgcca
    15541 tgaagcaaca gcgaggagtg tagtcgcaaa tggcgaaaga aaaaaaacgt ggcttttttt
    15601 cctggctggg ctttggtcaa aaagagcaga ccccggaaaa agagacagaa gttcagaatg
    15661 aacaaccggt tgtagaagaa atcgttcagg cgcaagagcc tgcgaaggct tcggaacaag
    15721 ccgttgaaga gcagccgcag gcgcatactg aggccgaagc ggaaactttt gctgccgacg
    15781 ttgtggaagt cactgaacag gttgttgaaa gtgaaaaagc gcaacctgaa gcggaagttg
    15841 ttgcacagcc ggaactggtc gtagaagaaa cgccggagcc agtggctatc gagcgtgaag
    15901 agctgccgtt gccggaagac gtcaacgccg aagaggtttc gccagaagag tggcaggccg
    15961 aagcggaaac cgtagagatt gtcgaagcgg cggaagaaga agcggctaaa gaagaaatca
    16021 ccgacgaaga gctggaagca caggcgctgg ctgccgaagc agcagaagag gcggtgatgg
    16081 tggttcctcc ggcagaagaa gagcagccgg tagaagaaat cgctcaggag caggaaaaac
    16141 cgaccaaaga aggtttcttc gcgcgcctga aacgcagcct gttaaaaact aaagaaaatc
    16201 tcggttccgg atttatcagc ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgaag
    16261 agctggaaga acagctgttg atcgccgatg tgggcgtgga aaccacacgt aaaataatca
    16321 ccaatctgac ggaaggcgca tcccgcaagc agcttcgtga tgccgaggcg ctttatggcc
    16381 tgctgaaaga agagatgggc gagattctgg cgaaagtcga tgagccgctg aatgttgaag
    16441 gtaaaacgcc gttcgtgatc ctgatggtgg gcgtcaacgg tgtgggtaaa accacgacga
    16501 ttggtaagct ggcacgtcag tttgagcagc agggtaaatc ggtgatgctg gcggcgggcg
    16561 atactttccg tgcggcagcg gttgaacagc ttcaggtctg gggtcagcgc aacaatattc
    16621 cggtgattgc ccagcatacc ggggcggatt ccgcctctgt tatcttcgac gccattcagg
    16681 cagctaaagc gcgtaatatc gacgtcctga ttgccgatac cgccggacgc ctgcagaaca
    16741 aatcgcacct gatggaagag ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg
    16801 aagcgccgca tgaagttatg ctgactattg atgccagcac cgggcagaac gcggtaagcc
    16861 aggccaaact gttccatgaa gccgttggct taaccggcat cacgctaacg aaactggacg
    16921 gcacggcgaa aggcggagta attttctcgg tggctgacca gtttggtatc cctatccgct
    16981 acattggtgt cggcgaacgt attgaggatt tgcgtccgtt taaggcggac gactttatag
    17041 aggcactttt tgcccgagag gattaacaat gattcgcttt gaacatgtca gcaaggctta
    17101 tctcggtggg agacaggcgc tgcagggcgt tacgttccat atgcagccgg gtgagatggc
    17161 gtttctgacc ggtcattccg gcgcagggaa aagtaccctc ctgaagctga tctgtgggat
    17221 tgagcggccc agcgccggga aaatctggtt tagcggccat gacatcacgc gtctgaaaaa
    17281 ccgtgaagtt ccgtttctgc gccgccagat tggtatgatt ttccaggatc accatctact
    17341 gatggaccgt actgtctacg ataacgtggc gatcccgctg attatcgccg gtgccagcgg
    17401 tgacgatatt cgtcgccggg tgtcggcggc actggataaa gtcgggctac tggacaaagc
    17461 gaagaacttt cctattcagc tttccggcgg tgaacaacag cgtgttggca ttgcccgcgc
    17521 ggtggtgaat aagcccgcgg tactgctggc ggacgaaccg actggtaacc tggacgacgc
    17581 gttgtcggaa ggcattttac gtctgtttga agagtttaac cgcgttgggg taaccgtatt
    17641 gatggcaacg cacgacatca acctgatttc gcggcgttcc tatcgcatgc tcaccctgag
    17701 cgatggtcac ttgcatggag gcgtgggcca tgaataagcg cgatgcaatc aatcatattc
    17761 ggcagtttgg cgggcgtctg gatcgcttcc gtaaatcggt cggcggctca ggcgacggcg
    17821 gtcgtaacgc accgaaacgc gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg
    17881 ttttcaacga acaggtgcgc tatgccttcc acggcgcatt gcaggatctg aaaagcaaac
    17941 cgttcgccac gtttttaacg gtgatggtta tcgccatctc cctgacgctg cccagcgtct
    18001 gttatatggt gtacaaaaac gttaaccagg cggcgacgca atattatccg tcaccgcaaa
    18061 tcactgttta tctgcaaaaa acgctggacg atgacgccgc tgcgggtgtg gtggcacagt
    18121 tacaggccga gcaaggcgta gagaaagtga attatctttc tcgtgaagac gcactgggcg
    18181 agttccgtaa ctggtctggt tttggtggtg cgctggatat gctggaagaa aacccgcttc
    18241 cggcagtggc ggtggtgatc ccgaaactcg attttcaggg gacggaatca ctgaatacgc
    18301 tgcgtgatcg tatcacccag atcaacggca ttgacgaagt gcggatggat gacagctggt
    18361 ttgcccgtct ggcggcgtta accgggctgg tcgggcgcgt ttccgcgatg atcggcgtgt
    18421 tgatggtggc agcagtgttc ctcgtcatcg gcaacagtgt gcgtctgagc atctttgctc
    18481 gccgtgactc cattaacgtg cagaaactga ttggtgcgac ggatggtttc atcctgcgcc
    18541 cgttcctgta cggaggcgca ttgctgggat tttctggcgc attgctgtca ttaattttgt
    18601 cagaaattct ggtactgcgt ttgtcatcgg cggttgcgga agtggcacag gttttcggaa
    18661 caaagtttga tatcaatggc ttatcgttcg atgaatgcct gctgttgctg ctggtatgct
    18721 cgatgattgg ctgggtggca gcgtggcttg ccacggtaca acatttacgc cactttacgc
    18781 cggaataata aaagcgtgtt atactctttc cctgcaatgg gttccgtagc agggaaagag
    18841 accccgttgt ctcttcccgg tatttcatct ctatgtcaca ttttgtgcgt aatttattca
    18901 caagcttgca ttgaacttgt ggataaaatc acggtctgat aaaacagtga atgataacct
    18961 cgttgctctt aagctctggc acagttgttg ctaccactga agcgccagaa gatatcgatt
    19021 gagaggattt gaatgactga caaaatgcaa agtttagctt tagccccagt tggcaacctg
    19081 gattcctaca tccgggcagc taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg
    19141 ctggctgaaa agctgcatta ccatggcgat ctggaagcag ctaaaacgct gatcctgtct
    19201 cacctgcggt ttgttgttca tattgctcgt aattatgcgg gctatggcct gccacaggcg
    19261 gatttgattc aggaaggtaa catcggcctg atgaaagcag tgcgccgttt taacccggaa
    19321 gtgggtgtgc gcctggtctc cttcgccgtc cactggatca aagcagagat ccacgaatac
    19381 gttctgcgta actggcgtat cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc
    19441 ttcaacctgc gtaaaaccaa gcagcgtctg ggctggttta atcaggatga agttgaaatg
    19501 gtggcccgtg aactgggcgt aaccagcaaa gacgtacgtg agatggagtc gcgtatggcg
    19561 gcacaggaca tgacctttga cctgtcttcc gatgacgatt ccgacagcca gccgatggct
    19621 ccggtgctct atctgcagga taaatcatct aactttgccg acggcattga agatgataat
    19681 tgggaagagc aagcggcaaa ccgtctgacc gacgcgatgc aaggtctgga cgagcgtagc
    19741 caggatatca tccgcgcgcg ctggctggat gaagacaaca agtccacgtt gcaggaactg
    19801 gctgaccgtt acggtgtttc cgctgagcgt gtgcgccagc tggaaaagaa tgcgatgaaa
    19861 aaacttcgcg ctgccattga agcgtaattt ccgctactca gcagagaacc ctggatgaga
    19921 atccggggtt tttgtttttt gggcctctgc aataatcagt ttccccctcc ggcaaaacgc
    19981 caatccccac gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc
    20041 gggtatgttt tagcagagta tgctgctaaa gcacgggtag tcatgcataa aacgaaataa
    20101 agtgctgaac aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat
    20161 gaacataaag ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc
    20221 tctggcagaa gatattaaag tcgccgtcgt aggcgcaatg tccggtccgg tggcgcagta
    20281 cggtgaccag gagtttactg gcgcagagca ggcggttgcg gatatcaatg ctaaaggtgg
    20341 tatcaaaggc aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc
    20401 ggttgcggtg gcgaacaaag tcgttaacga cggcattaaa tacgtgattg gtcacctctg
    20461 ctcttcatca acgcagccag cgtctgacat ctacgaagac gaaggcattt taatgatcac
    20521 cccagcggca accgcaccag agctgaccgc ccgtggctat cagctgatcc tgcgcaccac
    20581 cggcctggac tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc
    20641 gcagcgtatt gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cgcgagcggt
    20701 gcaggacggc ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg
    20761 ggaaaaagat ttctcaacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta
    20821 ctacggcggt tatcacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct
    20881 gaaaactcag tttatggggc cggaaggcgt ggctaacgtc tcgctgtcta acattgcggg
    20941 tgaatcagcg gaaggactgc tggtgaccaa gccgaagaac tacgatcagg ttccggcgaa
    21001 caaacccatt gttgacgcga tcaaagcgaa gaaacaggac ccaagcggcg cgttcgtctg
    21061 gaccacctac gccgcgctgc aatctttgca ggtgggcctg aatcagtctg acgatccggc
    21121 tgaaatagcc aaatacctga aagcgaactc cgtggatacg gtgatggggc cgctgacctg
    21181 ggatgagaaa ggtgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg
    21241 cacggcgact gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta
    21301 atatcgagta cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctacaagat
    21361 tcaaagaaat cagtaaattg cagcacacat tgtaggcctg ataagcgtag cgcatcaggc
    21421 aatatcactt gtgaaatcaa acttgacgat taacgcttct cccagccgcc ctgttgtgcc
    21481 gtaaatccca gtgcctgcat aaacgctgtc atcacgccgc gatcttccac gcctgcatcc
    21541 gccatccacc agcatgaaac gccaggattg ttacgcaaaa cctcttccag cagatattgc
    21601 cccacaccgc gacggcgggt gacttccctc acacgcaggg aatccagtgc cccctcggtg
    21661 ccgcttaagg ttacccgcac ggccgcgaga aggcgctcgt taaaacgcgc ggcgtagata
    21721 cggtggttat cgtcaacctg taaagaggaa ggggaatact ccggccagat cttttgcagg
    21781 tcaatccggt cctggtcgct aaagttttct aatcgaatga tggtcagctt catggggaac
    21841 ccgtgtaaat cacaaaagtg gaaccagtgt agcgaaat
//
LOCUS       DAZGAP010000082        21828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGAP010000082 DAZGAP010000000
VERSION     DAZGAP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.082"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1080
                     /gene="chaA"
                     /locus_tag="ACHLAM_002657"
     CDS             <1..1080
                     /gene="chaA"
                     /locus_tag="ACHLAM_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO7854163.1"
                     /translation="VKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINLLALIGIL
                     SSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTLMRDTLYS
                     IIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMALPAANFS
                     TGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAHSSLWHAI
                     WLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGALKAVLNNQ
                     VQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLVLCHISFS
                     TGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1485..1592
                     /gene="ldrA"
                     /locus_tag="ACHLAM_002658"
     CDS             1485..1592
                     /gene="ldrA"
                     /locus_tag="ACHLAM_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7854164.1"
                     /translation="MTLAQFTVIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(1741..2595)
                     /gene="kdsA"
                     /locus_tag="ACHLAM_002659"
     CDS             complement(1741..2595)
                     /gene="kdsA"
                     /locus_tag="ACHLAM_002659"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO7854165.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(2631..3440)
                     /gene="sirB1"
                     /locus_tag="ACHLAM_002660"
     CDS             complement(2631..3440)
                     /gene="sirB1"
                     /locus_tag="ACHLAM_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO7854166.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(3444..3836)
                     /gene="sirB2"
                     /locus_tag="ACHLAM_002661"
     CDS             complement(3444..3836)
                     /gene="sirB2"
                     /locus_tag="ACHLAM_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO7854167.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(3833..4666)
                     /gene="prmC"
                     /locus_tag="ACHLAM_002662"
     CDS             complement(3833..4666)
                     /gene="prmC"
                     /locus_tag="ACHLAM_002662"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7854168.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(4666..5748)
                     /gene="prfA"
                     /locus_tag="ACHLAM_002663"
     CDS             complement(4666..5748)
                     /gene="prfA"
                     /locus_tag="ACHLAM_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO7854169.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(5790..7046)
                     /gene="hemA"
                     /locus_tag="ACHLAM_002664"
     CDS             complement(5790..7046)
                     /gene="hemA"
                     /locus_tag="ACHLAM_002664"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO7854170.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDGLGLE"
     gene            7260..7883
                     /gene="lolB"
                     /locus_tag="ACHLAM_002665"
     CDS             7260..7883
                     /gene="lolB"
                     /locus_tag="ACHLAM_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO7854171.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPSNMELTDGGQRIKLKMDNWIVK"
     gene            7883..8734
                     /gene="ispE"
                     /locus_tag="ACHLAM_002666"
     CDS             7883..8734
                     /gene="ispE"
                     /locus_tag="ACHLAM_002666"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO7854172.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGAEISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            8885..9832
                     /gene="prs"
                     /locus_tag="ACHLAM_002667"
     CDS             8885..9832
                     /gene="prs"
                     /locus_tag="ACHLAM_002667"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO7854173.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            9957..11636
                     /gene="dauA"
                     /locus_tag="ACHLAM_002668"
     CDS             9957..11636
                     /gene="dauA"
                     /locus_tag="ACHLAM_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO7854174.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(11691..11969)
                     /gene="ychH"
                     /locus_tag="ACHLAM_002669"
     CDS             complement(11691..11969)
                     /gene="ychH"
                     /locus_tag="ACHLAM_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO7854175.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            12247..12831
                     /gene="pth"
                     /locus_tag="ACHLAM_002670"
     CDS             12247..12831
                     /gene="pth"
                     /locus_tag="ACHLAM_002670"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO7854176.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEIWFTDGLTKATNRLHAFKAQ"
     gene            12948..14039
                     /gene="ychF"
                     /locus_tag="ACHLAM_002671"
     CDS             12948..14039
                     /gene="ychF"
                     /locus_tag="ACHLAM_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO7854177.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEEREEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            14230..14406
                     /locus_tag="ACHLAM_002672"
     CDS             14230..14406
                     /locus_tag="ACHLAM_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854178.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(14443..16362)
                     /gene="dhaR"
                     /locus_tag="ACHLAM_002673"
                     /pseudo
     CDS             complement(14443..16362)
                     /gene="dhaR"
                     /locus_tag="ACHLAM_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
     gene            16590..17660
                     /gene="dhaK"
                     /locus_tag="ACHLAM_002674"
     CDS             16590..17660
                     /gene="dhaK"
                     /locus_tag="ACHLAM_002674"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO7854179.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRSDAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            17671..18303
                     /gene="dhaL"
                     /locus_tag="ACHLAM_002675"
     CDS             17671..18303
                     /gene="dhaL"
                     /locus_tag="ACHLAM_002675"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO7854180.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            18314..19732
                     /gene="dhaM"
                     /locus_tag="ACHLAM_002676"
     CDS             18314..19732
                     /gene="dhaM"
                     /locus_tag="ACHLAM_002676"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO7854181.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            20053..21750
                     /gene="treA"
                     /locus_tag="ACHLAM_002677"
     CDS             20053..21750
                     /gene="treA"
                     /locus_tag="ACHLAM_002677"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7854182.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
BASE COUNT         5310 a         5646 c         5489 g         5383 t
ORIGIN      
        1 gtaaaaaccc gccacaagga gacttcgctt attttcccgg ttctggcgct ggtagtgctg
       61 ttcctgtggg gaagcagcca gacactacca gtggtcattg ccatcaatct tcttgctctt
      121 attggtattt taagtagcgc ctttagtgtt gtccgtcatg cggacgtatt agcccatcgc
      181 ctgggagagc cttacggttc gcttattctt agcctttcag tggttattct tgaagtcagt
      241 ttgatttcag ctttaatggc aaccggcgac gccgcgccaa cgctaatgcg tgatacgctc
      301 tattcaatca ttatgattgt taccggtggg ctggttggct tttcattatt gttgggcgga
      361 cgcaagtttg ccacccaata tatgaatctg tttggtatca agcagtattt aattgcgctg
      421 ttccccctgg cgataatcgt actggtattt ccaatggctc tgcctgcggc gaatttttca
      481 accggtcagg cgttactggt agcattaatt tctgcggcaa tgtatggcgt atttttgctg
      541 atccagacca aaacgcatca aagtttgttt gtctacgagc acgaagatga cagtgatgac
      601 gacgacccgc atcacggtaa accgtctgcc catagcagcc tgtggcatgc tatctggttg
      661 attgtccatc tgattgccgt tattgcggtg accaaaatga acgccagccc gctggagaca
      721 ttgctcgaca gcatgaatgc ccctgtcgcc tttactggct tcctggtggc tctgttgatt
      781 ctgtcgccgg aaggtttagg tgcgttaaaa gcagtgttga acaaccaggt ccagcgcgcg
      841 atgaatctgt tctttggttc agtgttagca accatttcgc ttaccgtacc tgtcgtcacg
      901 ctaattgcct ttatgacggg taacgaattg cagtttgcac ttggtgcgcc agaaatggtg
      961 gtgatggtgg cctctttagt gctgtgtcat atctccttct ccaccggacg tactaacgtg
     1021 ctcaatggcg cagcgcatct ggcactgttt gccgcctatt tgatgacgat atttgcctga
     1081 cagttatatc tcctccgatg acggaaatcg ggggagataa tataaattcc tgcttattgt
     1141 atttcttcat actacctgga caggttgtta atcagaattg cgagcctgaa ccacaacctg
     1201 cacgaaacga ctgtaacagc tgttgaaaaa ccggaatgca ctcttcattg caaacatttc
     1261 agggatgaag tgaagatgaa ttgagtcgta gtatccacac caagccggcg ttatccggtg
     1321 aggcgcaatg ttgcgggggc tttatccctg gtggcattgg ttgctggaaa gagaaaaccc
     1381 ccgcacgttg agaggtataa cctgacaaca gaacgggggc taatcttgaa ccagaacaat
     1441 tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg ctatatgacg ctcgcgcagt
     1501 ttaccgtgat tttctggcac gacctggcag caccgatcct ggcgggaatt attaccgcag
     1561 cgattgtcgg ctggtggcgt aaccggaagt aaggcgaaaa cgggcgtcag gctgccgtga
     1621 tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc tgcggccctt tttgtatccg
     1681 gcccccatga aaaaagcctt aacgcagaac gctaaggctt tagttttcag gcaaaaaaga
     1741 ttacttgctg gtatccagtt cttcgaaacc tttcaccaag tcatcaatcg ctttcatctg
     1801 cttgaggaac ggttccagtt tagccagcgg cagcgcggat ggaccatcac atttcgcatg
     1861 ttccggatcc ggatgcgctt caataaacag ccccgccaga cctaccgcca taccggcacg
     1921 tgccagctca gccacctgag cacgacgacc gccggaagct gcgccaaacg gatcgcggca
     1981 ttgcagtgcg tgggtcacgt cgaaaatcac cggcgaatta ccagacactt tcttcatgat
     2041 gctgaagccc agcatatcga caaccaggtt gtcatagccg aagttagcac cgcgatcgca
     2101 aagaatcact ttttcgttgc cgccttcttt aaatttatca acgatattac ccatctgtcc
     2161 cgggctaaca aactgcggtt tcttgacgtt aattaccgca ccggttttcg ccatggcttc
     2221 caccaggtca gtctggcgag caagaaacgc cggcaactga atcacatcca cgacatccgc
     2281 aacgggctgt gcctgacttg gttcgtgaac gtcggtgata attttcacac caaaagtctg
     2341 cttcaactcc tggaagattt tcatcccttc ttccaggccc ggtccacgat aagagtggat
     2401 ggaggagcgg ttggctttgt caaaagaggc tttgaacacg taagggatac ccagtttctg
     2461 ggtcacggtt acgtagtgct cgcaaatgcg catcgccaga tcgcgagatt ccagcacgtt
     2521 cataccgcca aacagtacga acggcaggtc atttgctacg ttgatgtcgc caatgctaac
     2581 cactttttgt ttcataggat cgccttaatc ttgagtaaaa tgtcgattaa ttaatgcagc
     2641 acaatatgtt tatgcgcgat gttatttatt tgcgcacgga tcatttcgct gatcgggtct
     2701 tccggacact gttcaacgaa ataacttaaa tcgttcaacg caacgtgttc gcaatccagt
     2761 tgcgcataaa tcaacccgcg atcgcgaatt tcatagggat cttccgggtt gaattgtaat
     2821 aaagcttcgc tggtgcgtaa cgccagctcc atctgattct cttccatcaa cgaagctttg
     2881 agtgtatcca gcaatttgcg gattacctca atgttatcag cttcatcaag gtcttcataa
     2941 aacagttccg ccgacgggct gatatttccc tttaaccata cgtccagcat atgttcgctt
     3001 aacgattcac cgttaaaagg attaatcagc caaatttcgc catccggaca ttcaatgcgc
     3061 aatatcagct gcgtagggaa aatcaccggc agcagcggca aatcaagacg attcgcgacc
     3121 cataataaaa cggcacctaa tgataccgca ctgccctgtc gattctttaa cacctggtcc
     3181 agccataatg catcggaaag acgataaacg ccgcgtgagg ctttaaatcc ccagtcgccg
     3241 taaaacagcg cgatcagttt ttccagctgc tcttctaaag gcagaagctg gctgatttct
     3301 tccttcgcca gactaacgag acgctccagt tcgtcgtaaa catcttgcga gggaaaatcg
     3361 cggcggattg cttcgcaagc caggatcatg ccttcgcaca atggcgcttt attaaattcg
     3421 aaatcagcta acgatctcat gacttacccc agtaacggta cttttgtggt ggcgagttta
     3481 atgatgatgt acagcaccac caacgccagc gggaaggcaa taatgcgcgc ctgctgactg
     3541 tgcatacgac gataatcgag tgcaataaaa cccaaaacga tataaatgat aactccaaac
     3601 agcttttcag tcagccactg tgcctgcccg gaaaatggca ggatgtgcgc tttagccatc
     3661 aacgcaatgc cgcttaacaa taacagcgta tcgacaaccg gcggcacaat gcgcgtccag
     3721 cgcgcaaatg cctgtggatg cttctgataa cgtagccaga aacgtaaggt taatagccca
     3781 actgaaagcg cgatactaat aagatgaaca ctaagcagtg tagaaaaact tgtcattgat
     3841 aatagcggcc gagcgttacg cgctcgttat caccatagtc ccgacaggtt tcgacgtcat
     3901 gatatcccgc gtggataaat gcctgtcgca ccgcttcgcc ctgctgccaa ccatgttcca
     3961 gaagcagaaa gccgccggat accagcgcgt tacgcgactg ttcgatgata tgcacgatgt
     4021 ctgccattcc actgtctgcc gcaaccagcg cagtgagcgg ctcaaagcgg acatcgcctt
     4081 gttgaagatg tgggtcctgc tcgtcaatat acggcggatt actgacaatc atcgcaaact
     4141 gctgcccggc tagcgcgcta aaccagtcgc tttgcagaat atggatgttt ttgatcgcca
     4201 gattctgggc attacgttgc gccagggcga cagcatctgg catacgatct acagcggtaa
     4261 tttcgcagtc cggacgctcg ctggccagcg ccagcgcaat cgccccggta cccgtcccga
     4321 gatcgagaat acggcaaggt tgttcaggca accgcgccag tgcctgctcc accagacact
     4381 ccgtatccgg gcgcggaatt aaggtcgctg gcgaaacaaa taacggcaac gaccagaatt
     4441 ctcgcacccc ggttaaatga gcaatgggtt caccatcgcg acgacgcgtc agtagcgcat
     4501 caagttgctg acattgttcg tcagtcagct gcgtttcacc aaaggcgaga ataaaagtac
     4561 gccctctgcc ggtaacatgc tccagcagga tttcagcatc acgccgcggg ctttcgctcg
     4621 cctgaagttg gcttattgct tcacgtaacc agtgttgata ttccattatt cctgctcgga
     4681 caacgccgcc agttggtcgg cctgatgttc ctggataatc ggttcaatca gcatatccag
     4741 cttaccttcc atcacttcat ccaggcggta gagcgtcagg ttgatgcggt gatcggtaac
     4801 gcgcccctgt gggaagttgt aagtacggtt acggtcgctg cgatcgccac tccccagcag
     4861 gttacgacgg gtagacgctt cggcctgttg ccgttttgcc atttctgcag cgtggatgcg
     4921 agcaccgagt acagaaagtg ctttagcttt gtttttatgt tgtgaacgtt cgtcctgaca
     4981 ttcaacaaca atcccggtcg gcaagtgagt aatacgaatt gccgaatcgg tggtgttaac
     5041 gtgctgacca cccgcccctg acgagcggaa agtatcaatg cgcaaatctg ctgggttgat
     5101 gtccggcagt tctgcgtcag gcagttctgg cattaccgca acggtacaag cggaagtatg
     5161 aattcgaccc tgcgattccg tagcaggcac acgctgcacg cgatgaccgc cagattcgaa
     5221 tttcaaacga ccatatacac catcaccgct aattttggcg atgatctctt tataaccacc
     5281 atgttcaccc tcgctggcgc tcatgatttc tacacgccag cggcgggctt cggcgtaacg
     5341 gctgtacata cggaacagat cgccagcgaa gagcgccgct tcgtcgccgc cggttccggc
     5401 acggacttcg aggaaggcgt tacgttcgtc atcaggatct tttggtagta acagaacctg
     5461 taattgctgt tccagttgct cgcttttttc tttagcttcg cgcagttcat cctgcgccat
     5521 ctcacgcatt tcaggatcgt cgagcatcat ctgtgcggtt tcgatatctt cctgaacctg
     5581 ttgccagtcg gtaaaacagc gcgaaacatc acttaactgc gcatattcgc gtgataatgc
     5641 gcgaaaacgt tcctggtcgg cgatagtttg cgcgtcaccc agcaacgcct gaacttcttc
     5701 atggcgttca tgcagggctt ccagtttggc aacgatagaa ggcttcatag gcgtaaatgc
     5761 accctgtaaa aaaagaaaat gatgtactgc tactccagcc cgaggccgtc gcgcagaata
     5821 ttcaggcgtt cgttatcccc gtcacgggcg gcctgttgaa gtgatttcgt tggcgcatgg
     5881 atcaggcggt tagtcagttt ccatgccaga tcctgcataa tggcttgagc gtcaccgccc
     5941 tgctcaaggg ccgctaacgc tttggcagtt aactcatcgc gaacgtgctc tgcctggctg
     6001 cgatactcgc gaatggtttc gctggcgctt tgtgcacgca gccacgccat aaattcgctg
     6061 gtttcctgag cgacaatagt ttccgcctca accgctgcgg ctttacgctg cgccaggttg
     6121 tgcgaaatga tgctttgcag atcgtccacg ctataaagat aagcattcgc cagtttgcca
     6181 acttccggct caacatcgcg cggaacggca atatccacca acagcattgg ttggttgcga
     6241 cggcttttta atgcgcgctc caccatgcct ttcccgataa tcggtaacgg gctggcggtg
     6301 gaactgatga tgatatcggc ttcgcgcaga cgttcgtcga tatcactcag ggcaataact
     6361 tccgcgccga cttcatctgc cagaatttgg gcacgttcgc gagtgcggtt ggcgataatc
     6421 atcttctgta ctttgtgttc gcgcagatga cgcgccacca gctcgatggt ttcgcccgca
     6481 cccaccagca gtaccgtcac cgtagagagc gattcaaaga tctgccgcgc cagcgtacaa
     6541 gccgcaaaag cgacagaaac agcgctggca ccgatatctg tttcagtgcg aacacgtttc
     6601 gctacagaga aagatttctg aaacatgcgt tccagttcgc tggccttcat atggcctttt
     6661 tgcgaatcgg caaacgcttt tttaacctga ccgaggatct gcggctcccc caaaaccagt
     6721 gaatccaggc cgctggcaac acgcattaaa tggctaaccg cgtcgttatc ctgatgccag
     6781 tagaggcttt tacgcaggtc ttcttcatta agattgtgat aatcgcaaag ccagcggatt
     6841 aacgcctctt gcaggttatc ctgctcttca acgctaagat aaagttccgt gcgattgcac
     6901 gtcgacagca ccacgccgcc ctgcaccatc ggctgcgcaa gcaggctgtc aagcgcctga
     6961 tcgagcttat ccggcgaaaa cgatacacgt tctcgcagcg atacaggtgc cgttttatgg
     7021 ttgataccga gtgctaaaag ggtcatgtct gcgggaaata ataccaacgt tgatagggtt
     7081 agtctgcttg catcatacag gatgcgtaag atcaataaaa gagagcgccc ccttttggag
     7141 taattgccga aagccgttag attctggcaa ttaagacaac ttgaacatag acgatagcgg
     7201 acggtaacgc tagcattaag ggttataact gcaacgtatc tcaaggactt gtcatcacta
     7261 tgcccctgcc cgattttcgt cttatccgcc tactaccgct ggctgctctt gtgctcactg
     7321 cctgttccgt taccacgccc aaaggtcctg gcaaaagccc ggattcgcca caatggcgtc
     7381 agcatcagca agacgtgcgc aatcttaatc agtatcaaac tcgcggcgcg ttcgcttata
     7441 tttctgacca acaaaaagtg tacgcccgtt tcttctggca gcaaactggc caggatcgct
     7501 accgtctgct gctgactaac ccattgggca gcacggaact ggagctgaat gctcaaccgg
     7561 gtaacgtgca gttagtcgac aataaaggcc agcgttatac cgccgatgac gccgaagaga
     7621 tgattggcaa attgaccgga atgcccattc cactcaacag cttacgccag tggattttag
     7681 gtttgccggg tgatgcgact gactacaaac tggacgacca gtaccgcctt agcgaaatca
     7741 cctacagcca gaatggcaaa aactggaaag ttgtttacgg tggttatgac acaaaaactc
     7801 aacctgcgat gccatccaat atggaactca ccgatggtgg tcagcgcatc aagttaaaaa
     7861 tggataactg gatagtgaaa taatgcggac acagtggccc tctccggcaa aacttaatct
     7921 gtttttatac attaccggtc agcgtgcgga cggttaccac acgctgcaaa cgctgtttca
     7981 gttcctggat tacggcgaca ccatcagcat tgagcttcgt gacgatgggg atattcgtct
     8041 gttaacaccc gttgaaggcg tggaacatga agataacctg atcgttcgcg cagcacggtt
     8101 gctgatgaaa acagcggcag acagcgggcg tcttcctacg ggaagcggtg cagaaatcag
     8161 tattgacaag cgtttgccga tgggcggcgg tctgggtggt ggttcatcca atgccgcgac
     8221 ggtgctggtg gcattaaacc atctctggca atgcgggcta agcatggatg agctggcgga
     8281 aatggggctg acgctgggcg cagatgttcc tgtctttgtt cgggggcatg ccgcgtttgc
     8341 cgaaggcgtt ggtgaaatac taacgccggt ggatccgcca gagaagtggt acttggtggc
     8401 gcaccctggt gtaagtattc cgacaccggt gatttttaaa gatcctgaac tcccgcgcaa
     8461 tacgccaaaa aggtcaatag aaacgttgct aaaatgtgaa ttcagcaatg attgcgaggt
     8521 tatcgcaaga aaacgttttc gcgaggttga tgcggtgctt tcctggctgt tagaatacgc
     8581 cccgtcgcgc ctgactggga caggggcctg tgtctttgct gaatttgata cagagtctga
     8641 agcccgccag gtgctagagc aagccccgga atggctcaat ggctttgtgg cgaaaggcgt
     8701 taatctttcc ccattgcaca gagccatgct ttaagccggg caagctgagt ttcggtgaca
     8761 acgtcacctt gttccagacg ttgcatcgcg ctctttaata caccgcctgg aaagaatcat
     8821 gcctggcccg cacagttttc ggcagattct ttccaccaat ggacgcatgc ctgaggttct
     8881 tctcgtgcct gatatgaagc tttttgctgg taacgccacc ccggaactag cacaacgtat
     8941 tgccaaccgc ctgtacactt cactcggcga cgccgctgta ggtcgcttta gcgacggcga
     9001 agtcagcgta caaattaacg aaaatgtacg cggtggtgat attttcatca tccagtccac
     9061 ttgtgcccct actaacgaca acctgatgga attagtcgtt atggttgatg ccctgcgtcg
     9121 tgcttccgca ggtcgtatca ccgctgttat cccctacttt ggctatgcgc gccaggaccg
     9181 tcgcgtccgt tccgctcgtg taccaatcac tgcgaaagtg gttgcagact tcctctccag
     9241 cgtcggtgtt gaccgtgtgc tgacagtgga tctgcacgct gaacagattc agggtttctt
     9301 cgacgttccg gttgataacg tatttggtag cccgatcctg ctggaagaca tgctgcagct
     9361 gaatctggat aacccaattg tggtttctcc ggacatcggc ggcgttgtgc gtgcccgcgc
     9421 tatcgctaag ctgctgaacg ataccgatat ggcaatcatc gacaaacgtc gtccgcgtgc
     9481 gaacgtttcc caggtgatgc atatcatcgg tgacgttgca ggtcgtgact gcgtactggt
     9541 cgatgatatg atcgatactg gcggtacgct gtgtaaagct gctgaagctc tgaaagaacg
     9601 tggtgctaaa cgtgtatttg cgtacgcgac tcacccgatc ttctctggca acgcggcgaa
     9661 caacctgcgt aactctgtaa ttgatgaagt cgttgtctgc gataccattc cgctgagcga
     9721 tgaaatcaaa tcactgccga acgtgcgtac tctgaccctg tcaggtatgc tggccgaagc
     9781 gattcgtcgt atcagcaacg aagaatcgat ctctgccatg ttcgaacact aatcgaaccc
     9841 ggctcaaaga cccgctgcgg cgggtttttt tgtctgtaat atccatttgt atgacctatg
     9901 cctccttcac ctgccattta gttgacagat gatgcgctca cggatgaaac attattgtga
     9961 acaaaatatt ttcctcacat gtgatgcctt tccgcgctct gatcgacgct tgctggaaag
    10021 aaaaatatac tgccgcacgg tttacccgtg acctgattgc cgggataacc gtcgggatta
    10081 ttgctatccc gctggcaatg gcgttggcta ttggtagtgg tgtggcaccc cagtacggtt
    10141 tatataccgc agctgttgcg gggattgtca ttgctctgac gggtgggtca cgctttagcg
    10201 tttccggtcc gactgcggca tttgtggtaa ttctctatcc cgtttcgcaa cagtttggac
    10261 tggcaggact gctggtcgcg acactcatgt caggtgtctt tttgatcctg atggggctgg
    10321 cacgttttgg acgcttgatt gaatatattc cggtttccgt taccttaggt tttacttccg
    10381 gtatcgggat caccatcggt accatgcaga ttaaagattt tctcggtctg caaatggccc
    10441 atgtccctga acattatcta caaaaagtcg gcgcattatt tatggcgttg ccgaccatta
    10501 atgtgggtga tgctgccatt ggcattgtga cgcttggcat tctggttttc tggccacgtc
    10561 tgggcattcg tttacccggt caccttccgg ccttgctggc tggttgcgcg gtgatgggga
    10621 ttgttaacct gctcggcgga catgttgcta ccatcggttc gcaattccac tacgttctgg
    10681 ccgatggttc tcagggtaac ggtattccgc aactgctgcc gcaactggta ctgccgtggg
    10741 acctgcccaa ttccgaattt accctcacct gggactccat tcgcactctg cttccggcag
    10801 cattttcgat ggcgatgtta ggggcaattg aatcactgct ctgcgccgtg gtgctggatg
    10861 gtatgaccgg gacgaaacac aaagcgaaca gtgaactggt tggacaggga ctggggaata
    10921 tcatcgctcc gttctttggt ggtattaccg ctacagctgc catcgcgcgt tctgccgcta
    10981 acgtccgtgc cggggcaact tcccctatct cggcggtgat ccactctatt ctggttattc
    11041 ttgccctgct ggtactggca ccgctgctct cctggctgcc tctttccgcc atggctgccc
    11101 tgctgttgat ggtggcgtgg aacatgagtg aagcgcacaa agtggtcgac ttgctgcgtc
    11161 atgcgccgaa agatgacatc atcgtcatgc tgctgtgcat gtcgctgacc gtgctgtttg
    11221 atatggttat tgccatcagc gtggggatcg tgctggcatc gctactgttt atgcgtcgta
    11281 tcgcacgtat gactcgcctg gcaccggtag tcgtagacgt tccagacgat gttctggttc
    11341 tgcgcgttat tggcccgctg ttctttgctg ccgctgaagg cttgttcacg gacctggagt
    11401 cacgtcttga aggcaaacgg attgtgattc tgaagtggga tgccgttccg gtacttgatg
    11461 ctggtggtct tgatgcgttc cagcgttttg tgaagcgtct acccgaagga tgtgaactgc
    11521 gcgtgtgcaa cgtggaattc cagccactgc gcactatggc tcgcgcaggc attcaaccga
    11581 tcccgggacg cctggcgttc ttcccgaatc gtcgcgcggc gatggcggat ttataagtac
    11641 gaaattgacc agtcagcgca aactgactgg tcagcaaacc gcattatttg ttagctatga
    11701 cggcgattat cgctacggcg gcaacgtttg tcatagtggc gaacccacca gtaacggtcg
    11761 cacacctgtt catgcccgcc aacacgggca cccgccagcc agagaatggc accgacgaaa
    11821 atacttagca ctgcaccatg tgcgaaatac tggggcatat taaactgtgg taactggttg
    11881 aggattgaat accccacgcc gaccaccatt accaccagac ccaaccccat gagcacgtta
    11941 ccgagtaacg aagcgttttt gcgtttcata tgtcacctcc ggaactttct gggttgtaac
    12001 agggaatacc cctcttcctt atgtgtaaag tatagacaac accctgtggg ttatgtgcgg
    12061 gcgtgatcac aattacaacc cttatttcaa caaaacttta caaataaacg cctgaacacc
    12121 ttgactttct aatagtccac ttaggcaatt attgcgttat ctgcatttga tacgcagttt
    12181 tttgtcaagc ggggccgcaa acagtaaact acgcgccagt tatgtacaca ctcaggacaa
    12241 aaaaacgtga cgattaaatt gattgtcggc ctggcgaacc ccggcgctga atacgccgca
    12301 acgcgacata atgctggtgc ctggttcgtt gacttactgg cagagcgatt gcgcgctccg
    12361 ctgcgcgaag aggctaaatt ctttggttat acttcgcgag tcacccttgg aggcgaagat
    12421 gtccgcctgt tagtcccgac tacatttatg aatctcagcg gcaaagccgt tgcggcgatg
    12481 gccagttttt tccgcattaa tccggacgag attctggtgg cccacgacga actggatctg
    12541 cctcctggcg tcgccaaatt taagttgggc ggtggccatg gtggtcacaa tggactgaaa
    12601 gacatcatca gtaaattggg taataaccct aactttcacc gtttacgcat cggaattggt
    12661 catccaggcg ataaaaataa agttgtcggt tttgtgttag gcaaaccgcc tgttagtgaa
    12721 cagaagttaa ttgatgaagc catcgacgaa gcggcacgtt gtactgaaat atggtttaca
    12781 gatggcttga ccaaagcaac gaaccgattg cacgccttta aagcgcaata agtcgttgtc
    12841 tgcggcattt ttgccgagtg ccgtgtataa taggcaaagt tatttccatt tctgcaatct
    12901 gttagcaata acaggttgat tattaagata ttaaggtgat ttaaatcatg ggattcaaat
    12961 gcggtatcgt cggtttgccc aacgtcggga aatctaccct gttcaacgcg ctgaccaaag
    13021 ccggtattga agcggccaac tttccattct gtaccattga gccgaacacg ggcgtcgtac
    13081 caatgcctga ccctcgcctg gatcaactgg ctgaaatcgt aaaaccgcag cgtacgcttc
    13141 ccactaccat ggaatttgtc gatatcgccg gtctggtaaa gggcgcgtcg aaaggtgaag
    13201 gtctgggtaa ccagttcctg accaacattc gtgaaaccga agcgatcggc cacgttgttc
    13261 gctgctttga gaatgacaat atcattcacg tttccggtaa agttaacccg gcggacgata
    13321 ttgaagttat caataccgaa ctggcactgg cggatctcga cacctgcgag cgcgcgattc
    13381 accgcgtaca gaagaaagcc aaaggcggcg ataaagacgc gaaagccgaa ctggcagtac
    13441 tggaaaaatg ccttccacag ctggaaaacg cgggcatgct gcgcgcgctg gatttaagcg
    13501 cagaagaaaa agcggctatt cgttacctga gcttcctgac gctgaaaccg acgatgtaca
    13561 tcgccaacgt caacgaagac ggttttgaaa acaacccgta tcttgaccag gtgcgtgaaa
    13621 tcgcggcgaa agaaggttct gttgtggttc cggtttgtgc agctgttgaa gcagacattg
    13681 ccgaactgga cgatgaagaa cgtgaagagt ttatgcagga actgggactg gaagagccgg
    13741 gcctgaaccg tgtgatccgt gccggttata aactgctgaa cctgcaaact tacttcaccg
    13801 ctggggtgaa agaagtgcgc gcatggacca tccccgtcgg cgcaactgca ccgcaagcgg
    13861 ctggcaaaat ccataccgat ttcgaaaaag gctttatccg cgcacagact atctcgtttg
    13921 aagatttcat cacttacaaa ggtgaacaag gcgcgaaaga agcaggcaaa atgcgtgcag
    13981 aaggtaaaga ttacatcgtt aaagatggcg atgtgatgaa cttcctgttc aacgtctaaa
    14041 ttccacgtaa taccctgacg gttaataatg agccattatg tgagtcgcac ataatggctt
    14101 ttttacgcct gctacgcggc ttctttcaat tctgagttat cgaaaatcat aatcagactc
    14161 ctggcaatat taacaatgtg ccttttatgt gataacaatc acagtgcatt aaccgttcaa
    14221 gagtttgtca tgaaaaaaac atttaccttc agcctgttag caattgtctt tgcgagcctg
    14281 ttaagtgctt gtgttcccca ccatcatcac cgccacagcg atggccctcg tggtcctgcg
    14341 ccctccggta agccaatgcc accttcaaat ggtggaccaa atcacggcag tcatggtcgc
    14401 tattaattag caattcatag cgggaaagta catactctcc cgctatccac ggcgcttaaa
    14461 ctgccctgca tcaatgccat gttgcttcat tttccgccac agcgtagtgc ggccgattcc
    14521 aagtaaagcc gacatttcct gaatgcgacc gcctgtgacc tgggctgcgt taataattgc
    14581 ctctttttca acttccgcaa atgacagact ggtggaaagg cgagtggcgc tgacatcatc
    14641 tgtcgcctgc tcggtaaaga gatgttcagg taaatcactg acgcgaatgc gcccgttatc
    14701 actactcaga gcaagattct cgatgacgct gtaaagttca aaatcgttgc caggccatgc
    14761 acaggaaacc aggcgagcga gggcatcgtc atcaattttc agccgcgtag agaagcgttt
    14821 ttcaagactg tgtaatttgt tattcaccag cgccggaatg ctgccacgcc gcatacgcag
    14881 aggcgggatg gtaatttcaa atgcatgcag cgcgtaatac agctggcgac taaaacgatt
    14941 ttgttccacc agcattgcca ggtccgcggt cgttgtggca atcactttga catcaatggg
    15001 tattaaacgc cgcgcatcca gtcgcgtgat aacaccctgc ttgataacct gaagcaaagc
    15061 agactgtaac tccaccgcca gatattcaat cttttcaaga aacagcgtgc cgccgtgcgc
    15121 cagttccagt cgactcaaac gaccattttc attgtccgtg cgatcgccgc caataaattc
    15181 ttccgccagc gccgcatcac catataactc acaattgacg gcgatataag gccctgctgc
    15241 acgctcactt tcattatgaa ttgcctgact tagcagtgcc ttgcccacgc cctcttctcc
    15301 acaaagcagg acaggaaagc tactgcgcgc cgcctggcga ccaaaatgaa tcaaacggcg
    15361 ggtttgcgga tcctcttgtg gcatatgagc gaaagtatgg ctgacttttc ctaattgact
    15421 ggtcattaac tgccgcatct gttccacagg atggagcaac aaaataaagc tggttccctg
    15481 cgtttctatt atcggtttaa gggttatcac cgcatcaata aactggtgct ggctttcaaa
    15541 ggttgcttct acgtgtttga gcggatgtgc ctgttttatt gcttgttgca atacggcggg
    15601 taacgttaag agttcagtga ttgcccgtcc ctgacttgcc gtcgcgtcaa ggcgtaagac
    15661 ccgcgccgcc tgggcattaa taaattgcaa attaccctgc tcgtcccagc taatcacgcc
    15721 atcatccata ctttctaaca gggcattaag ttgatttaaa tgacggttag tttcagcgag
    15781 caaactgtcc gtcagcagta aatttccgac ctcgcgtgcg attgccaacg tcaacggcaa
    15841 atcagctgcg gtagtttgct caaccggaca cgccagcgct attgttcccg tcaatcggcc
    15901 cttgctgtca aacaacggcg ttgcacaaaa ggcccagttc cagagtgcct gtttgaaatg
    15961 ttgatcggcc atcgttttca cggcctgacc agagatagcc gctaacgata gcgcacaagt
    16021 accaataatt ccctcggcgc aatacgtgcc gtcattgaat cccagtgcac ttagttgcta
    16081 caaggtttgc ggatcgccat tacggctgag aatgcaggcg gtttcatcga ggataagcag
    16141 cgcacacgct cgcggtgcca tatattccca ggcatcttcc agcgcagcct gcccgagcgt
    16201 cagcatcgct ttcttacgcc gataaataga cgcaaatgtc acgccctggg cctggtgtgg
    16261 tacgttccat gtatcccgtt tcatcagctt aatgcatcgc tcccaggagg ttgcaattaa
    16321 gggagatatg tcccgaccat cgttgttaaa agcgctactc atatcccgca tcccttatga
    16381 caaaaggtgt tccggctcgc aattttaagt attaatacac gtaaaagttt caatatggaa
    16441 cattcattaa agaatatgtt tcactatgaa acataatttg ggctaatttt cttttccctt
    16501 gcctgatgca caatggattc atcggcagtc agtggtcgcc gtgtcgttga acatcatcca
    16561 tgccctaccg taattgctgg agcaaaataa tgaaaaaatt gatcaatgat gtgcaagacg
    16621 tactggacga acaactggca ggactagcga aagcgcatcc atctctgaca ctgcaccagg
    16681 atccggtgta tgtcacccga tctgatgccc ccgttgcagg taaagtcgcc ctgctgtcgg
    16741 gtggcggcag cggacacgag ccgatgcatt gtggttatat cggtcagggg atgctttcgg
    16801 gggcctgtcc gggcgaaatt ttcacctcac cgacgcccga taaaatcttt gaatgcgcca
    16861 tgcaaattga tggcggcgaa ggtgtactgt tgattatcaa aaattacacc ggcgatattc
    16921 ttaactttga aacagcgacc gagttactgc acgatagcgg cgtaaaagtg accactgtgg
    16981 tcattgatga cgacgttgcg gtaaaagaca gtctttatac cgccgggcga cgcggcgttg
    17041 ccaacaccgt attaattgaa aaactcgtag gcgcagcggc cgagcgtggc gactcactgg
    17101 acgcctgtgc ggaactgggg cgtaagttga ataatcaagg ccactcaata ggtatcgctc
    17161 tcggtgcctg taccgttcct gccgcgggca aaccttcttt taccctggcg gacaatgaga
    17221 tggagtttgg cgtcggcatt catggtgagc caggtattga ccgccgtccc ttctcttccc
    17281 ttgatcaaac cgtcgatgaa atgttcgaca ccctgctgga aaatggctca taccatcgca
    17341 ctttgcgttt ctgggattat caacaaggca gttggcagga agaatcacaa actaaacaac
    17401 cgctccagtc tggcgatcgg gtgattgcgc tggttaacaa tcttggcgca actccgcttt
    17461 ctgagctgta cggcgtctat aaccgcctga ccacacgttg ccagcaagcg ggattgacta
    17521 tcgagcgtaa tttaattggc gcgtactgca cctcactgga tatgaccggt ttctcaatca
    17581 ccttactgaa agttgatgac gaaacgctgg cactctggga cgccccggtc cacaccccgg
    17641 cccttaactg gggtaaataa ggagatagca atgtcactga gcagaactca aattgttaac
    17701 tggctcactc gttgtggcga tattttcagc accgagagcg agtatcttac cggactggat
    17761 cgcgaaattg gcgacgctga ccacgggcta aatatgaacc gaggctttag caaagtggtg
    17821 gaaaaactcc ctgctatcgc agataaagat atcggtttca ttctcaagaa taccggtatg
    17881 acgctacttt ccagcgtcgg tggtgccagt ggtccgcttt tcggtacctt ctttatccgc
    17941 gccgcacagg cgacccaggc acggcaaagc ctgacgctgg aagagcttta tcagatgttc
    18001 cgcgatggcg cggacggcgt aatcagtcgc gggaaagccg aacctggcga taaaaccatg
    18061 tgtgatgtgt gggtgccggt ggtggaatcg ttacgtcagt ccagcgagca aaatctctct
    18121 attccggcgg cgctcgaagc tgccagtagc gtcgccgaat ccgctgcaca aagtacgatt
    18181 acgatgcaag ctcgcaaagg ccgtgccagt tatctcggtg aacgcagtat tggtcaccag
    18241 gaccccggtg cgacctcggt gatgtttatg atgcaaatgt tggcgttagc cgcaaaagag
    18301 taaggaattg gtgatggtaa acctggtcat agtttcacat agcagccgac tgggagaagg
    18361 cgtcggtgaa ttagcccgtc agatgttaat gagtgataat tgtaaaatcg ccattgccgc
    18421 gggaattgac gatccacaaa atcccattgg taccgatgcc gtcaaagtga tggaggccat
    18481 cgaatctgtt gccgataccg accatgtgct ggtcatgatg gatataggaa gcgcattatt
    18541 gagtgctgaa actgcgctgg aattgctggc tcccgagatc gccgcaaaag tacgtttgtg
    18601 tgctgcgccg ttggtcgaag gtacactggc agcaacggtt agcgcggcct cgggggcgga
    18661 gatcgacaaa gtgatctttg acgccatgca tgcgctggaa gccaaacgtg aacaactggg
    18721 tttaccctcc tccaacactg aaatctctga cacatgtcct tcgtacgatg aagaagcccg
    18781 ttctctggcg gtggtcataa aaaaccgtaa cggcctgcat gtacgtccgg cctcccggct
    18841 ggtttatacc ttatcgacat ttaatgccga tatgttgctg gagaaaaacg gcaaatgcgt
    18901 cacaccggag agtattaacc agattgcgtt actacaagtt cgctataacg atacgctgcg
    18961 cctgattgcg aaagggccag aagctgaaga ggcactgatc gctttccgtc agctggctga
    19021 agataacttt ggtgaaacgg aggaagttgc tccacctatt ctgcgtcccg ttccgcctgt
    19081 ttcgggtaaa gccttttatt atcaaccggt tttatgtacg gtacaggcaa aatcaaccct
    19141 gaccgtggat gaagaacaag aacgattacg ccaggccatt gacttcacgt tattagatct
    19201 gatgacgtta acagcgaaag cagaagccag cgggcttgac gatattgccg caatcttttc
    19261 tggtcaccat acactgttag atgatccgga actgctggcg gcggcaagcg aactccttca
    19321 gcatgaacat tgcacggcag aatatgcctg gcagcaagtt cttaaagaac ttagccagca
    19381 ataccagcaa ctggatgatg aatatctaca ggctcgctat attgatgtgg acgatcttct
    19441 gcatcgcacc ctggtccacc tgacccaaac gaaagaagaa ctcccgcagt ttaactcgcc
    19501 aactattcta ctggcggaga acatctatcc ctccacaata ctgcaactgg atccggcggt
    19561 tgtaaaaggt atctgcctta gcgccggaag tccgctatcc cacagcgccc tgatcgcccg
    19621 tgaactgggg attggctgga tttgccagca gggtgagaaa ctgtatgcga tacaaccaga
    19681 agaaacgcta acgctggacg ttaaaacgca acgtttcagc cgtcagggtt aaaccaccgt
    19741 tcggatggca tcgttctgat gccatccggc tatttactct gcacggatga tttttaacta
    19801 cctgttatat tttttaccaa atcatgatga ttttttctgc gctaatcgcg aacccaacgc
    19861 cgctggctgg ctaacaaaca gacgttattt gatcactcta tttcgacgtt atttatccct
    19921 ttaattccct tttctaaaat gcctgaccgt tcgcagaatg agatttcgat catgcagcta
    19981 gtgcgatcct gaactaaggt tttctgatac ttgaataccg ttttattcgg tttcgccaaa
    20041 ggagaatgat tgatgaaatc ccccgcagct tctcgcccgc taaaaatggc gttaattcca
    20101 gcctgcatct ttttgtgttt cgctgcgcta tcggtgcagg cagaagaaac accggtaacg
    20161 ccacagccgc ctgatatttt attagggccg ctgtttaatg atgtgcaaaa cgccaaactt
    20221 tttccggacc aaaaaacctt tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt
    20281 gctgattatc ggatgcagca aaaccagagc ggatttgatc tacgccattt cgttaacgtc
    20341 aatttcaccc tgccgaaaga gggcgagaaa tatgttccgc cagaggggca atcactacgc
    20401 gaacatattg acggactttg gccggtatta acgcgttcta ccgaaaatac cgaaaaatgg
    20461 gattctctgt taccgctgcc ggaaccttat gttgtgccgg gcggacgttt tcgcgaggta
    20521 tattactggg acagttactt caccatgtta gggcttgccg aaagtggtca ctgggataaa
    20581 gtcgcggata tggtggccaa ttttgctcat gaaatagaca cttacgggca tattcccaac
    20641 ggcaaccgca gttactattt aagccgctca caaccgccat tctttgccct gatggtagag
    20701 ttactggcgc agcatgaagg cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa
    20761 gaatatgctt actggatgga cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc
    20821 gttgtcaaac ttcaggatgg tacccttctc aaccgctact gggacgatcg cgatacgcca
    20881 cgaccagagt catgggtgga agatattgcc accgccaaaa gcaatccgaa tcgacctgcc
    20941 actgaaattt accgcgacct gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc
    21001 tggatggaca atccgcagca gttaaatacc ttacgcacca ccagcatcgt accggtcgat
    21061 ctgaacagcc tgatgtttaa aatggaaaaa atcctcgccc gcgccagcaa agctgccgga
    21121 gataacgcga tggcaaacca gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa
    21181 aaatacctgt ggaacgatca acaaggctgg tatgccgatt acgacctgaa aagtcataaa
    21241 gtgcgcaatc agttaaccgc ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa
    21301 gatcgcgcca gcaaaatggc gacggcgacg aaaacacatc tgctgcaacc aggcggcctg
    21361 aacaccacgt cggtgaaaag tgggcaacaa tgggatgcgc caaacggctg ggcaccgttg
    21421 cagtgggtcg cgacagaagg attacaaaac tacgggcaaa aagaggtggc gatggacatt
    21481 agctggcact tcctgaccaa tgttcagcac acctatgacc gggagaaaaa gctggtggaa
    21541 aaatatgatg tcagcaccac cggaacaggg ggcggcggtg gcgaatatcc attacaggat
    21601 ggctttggct ggaccaatgg cgtgacgctg aaaatgctgg atttgatctg cccgaaagag
    21661 caaccgtgcg acaatgttcc ggcgacgcgt ccactcagtg aatcaacaac acagccggta
    21721 aaacaaaatg aggcggaacc tacgccttaa ctttactgta tatcgagcaa tgccattacg
    21781 tgcataaaat tggcattgct ctttcttcac cacgaaatac caaatacc
//
LOCUS       DAZGAP010000083        21748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGAP010000083 DAZGAP010000000
VERSION     DAZGAP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.083"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..1304
                     /gene="ynfM"
                     /locus_tag="ACHLAM_002678"
     CDS             51..1304
                     /gene="ynfM"
                     /locus_tag="ACHLAM_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854183.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(1354..1473)
                     /locus_tag="ACHLAM_002679"
     CDS             complement(1354..1473)
                     /locus_tag="ACHLAM_002679"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO7854184.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1729..2037
                     /gene="asr"
                     /locus_tag="ACHLAM_002680"
     CDS             1729..2037
                     /gene="asr"
                     /locus_tag="ACHLAM_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO7854185.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            2130..2213
                     /locus_tag="ACHLAM_002681"
     CDS             2130..2213
                     /locus_tag="ACHLAM_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854186.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2313..3134
                     /gene="ydgD"
                     /locus_tag="ACHLAM_002682"
     CDS             2313..3134
                     /gene="ydgD"
                     /locus_tag="ACHLAM_002682"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO7854187.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3173..3502)
                     /gene="mdtI"
                     /locus_tag="ACHLAM_002683"
     CDS             complement(3173..3502)
                     /gene="mdtI"
                     /locus_tag="ACHLAM_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO7854188.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3489..3854)
                     /gene="mdtJ"
                     /locus_tag="ACHLAM_002684"
     CDS             complement(3489..3854)
                     /gene="mdtJ"
                     /locus_tag="ACHLAM_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO7854189.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4265..5299
                     /gene="tqsA"
                     /locus_tag="ACHLAM_002685"
     CDS             4265..5299
                     /gene="tqsA"
                     /locus_tag="ACHLAM_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO7854190.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLQQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5324..6712)
                     /gene="pntB"
                     /locus_tag="ACHLAM_002686"
     CDS             complement(5324..6712)
                     /gene="pntB"
                     /locus_tag="ACHLAM_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO7854191.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATILGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6723..8255)
                     /gene="pntA"
                     /locus_tag="ACHLAM_002687"
     CDS             complement(6723..8255)
                     /gene="pntA"
                     /locus_tag="ACHLAM_002687"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO7854192.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFTAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8779..9723
                     /gene="ydgH"
                     /locus_tag="ACHLAM_002688"
     CDS             8779..9723
                     /gene="ydgH"
                     /locus_tag="ACHLAM_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO7854193.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9909..11291
                     /gene="ydgI"
                     /locus_tag="ACHLAM_002689"
     CDS             9909..11291
                     /gene="ydgI"
                     /locus_tag="ACHLAM_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7854194.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLTFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11328..12050
                     /gene="folM"
                     /locus_tag="ACHLAM_002690"
     CDS             11328..12050
                     /gene="folM"
                     /locus_tag="ACHLAM_002690"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO7854195.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12047..12382)
                     /gene="ydgC"
                     /locus_tag="ACHLAM_002691"
     CDS             complement(12047..12382)
                     /gene="ydgC"
                     /locus_tag="ACHLAM_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO7854196.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12511..13230
                     /gene="rstA"
                     /locus_tag="ACHLAM_002692"
     CDS             12511..13230
                     /gene="rstA"
                     /locus_tag="ACHLAM_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO7854197.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILQE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13234..14535
                     /gene="rstB"
                     /locus_tag="ACHLAM_002693"
     CDS             13234..14535
                     /gene="rstB"
                     /locus_tag="ACHLAM_002693"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO7854198.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14611..15540
                     /gene="tus"
                     /locus_tag="ACHLAM_002694"
     CDS             14611..15540
                     /gene="tus"
                     /locus_tag="ACHLAM_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO7854199.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKTAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15537..16940)
                     /gene="fumC"
                     /locus_tag="ACHLAM_002695"
     CDS             complement(15537..16940)
                     /gene="fumC"
                     /locus_tag="ACHLAM_002695"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO7854200.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(17083..18729)
                     /gene="fumB"
                     /locus_tag="ACHLAM_002696"
     CDS             complement(17083..18729)
                     /gene="fumB"
                     /locus_tag="ACHLAM_002696"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7854201.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18928..20103
                     /gene="manA"
                     /locus_tag="ACHLAM_002697"
     CDS             18928..20103
                     /gene="manA"
                     /locus_tag="ACHLAM_002697"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO7854202.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            20204..21712
                     /gene="ydgA"
                     /locus_tag="ACHLAM_002698"
     CDS             20204..21712
                     /gene="ydgA"
                     /locus_tag="ACHLAM_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7854203.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
BASE COUNT         5349 a         5587 c         5308 g         5504 t
ORIGIN      
        1 ctaatctatt cataagcctg aaccacaggc atatttagca aggatttcaa gtgagccgta
       61 ctacaactgt tgatggcgct ccggcaagcg acactgacaa gcaaagcatt tctcagccaa
      121 atcaatttat taaacgcggt acgccgcaat ttatgcgcgt caccctggcg ctgttctctg
      181 ccggactggc aaccttcgca cttctctatt gtgtgcaacc tatccttccg gtgctttcgc
      241 aggagtttgg cttaacgccc gcgaacagta gtatttcact gtccatttct acggcgatgt
      301 tggctatcgg tttgctgttt actggcccgt tatccgatgc cattggtcgc aaaccagtga
      361 tggtcacggc gctactgttg gcctccattt gtacgttact ttcgacaatg atgaccagct
      421 ggcacggcat tttgattatg cgcgccttga ttgggctttc gttaagtggc gtagcagctg
      481 ttggcatgac ttatcttagc gaggaaatcc accccagttt cgtggccttt tcgatggggt
      541 tgtatatcag cggcaactca attggcggca tgagcggacg cttaattagc ggtgtcttca
      601 cggacttttt caactggcga attgcgctgg cggcaatcgg ttgtttcgcg ctggcctcgg
      661 cgttaatgtt ctggaaaatc ctccctgaat cacgccattt tcgcccgact tcactgcgcc
      721 ctaagacgtt attcatcaac tttcgtctgc actggcgtga ccggggatta ccgttattgt
      781 tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct gtttaattac gtcggctatc
      841 ggttgatgct ctccccctgg catgtcagcc aggccgtggt tggcttatta tcgctggctt
      901 atttgaccgg tacatggagc tcacccaaag ccggaaccat gaccacccgc tatgggcgcg
      961 gtccggtgat gttgttttcg acgggggtta tgctgtttgg tttattgatg accttattca
     1021 gctcgctgtg gctgatcttt gccggaatgt tactcttctc agccggattc ttcgcagccc
     1081 actcagtagc cagcagctgg atcggccccc gcgcaaaacg cgctaaaggc caggcctcct
     1141 cgctgtatct gttcagttac tatctggggt cgagtattgc cgggacgctg ggtggtgttt
     1201 tctggcataa ctatggctgg aacggcgtcg gcgcatttat tgctctgatg ctggtcattg
     1261 ctctgctggt cgggacgcgt ttgcatcatc gtctgcacgc ctgaaaaaat aagtccggac
     1321 tgaggtaaat acccgtccgg acttattgcc tgctcaaacc aacgttaata gccatcctaa
     1381 aatagacgaa gcgccagcca attcccgcag cgcatctagt gtcatcagga ttataagtac
     1441 ccaaataaac ggattcattt tgctgtgtgt catttattac tgatgcgcag ttattctacc
     1501 gctttgtaag tagtaaaata gttaacacga tcaagactac tattattggt agatgaattt
     1561 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
     1621 ttacacgctg aaaccaacca ctcacggaag tctgccattc tcagggatat agttatttca
     1681 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
     1741 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ctgcctttgc tgcagagact
     1801 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgcctgcgaa aaccacacat
     1861 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
     1921 aaaaatgcca aagctgaaca gaaagctcct gaacaaaaag cgcaggcagc gaagaaacac
     1981 gccaagaaac acagccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
     2041 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta aacgggtaac
     2101 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
     2161 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgatcgtagt
     2221 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
     2281 ttaataatca taattaccca ccagagtgtg atatgcgtag aaccattgct gtagtgttgg
     2341 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
     2401 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
     2461 cgacccaatc accgtgggat gcggttggcc aactggaaac ggccagcggc aatttatgta
     2521 cagcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     2581 caaagggtaa agccgataaa gcggtggcgc tgcgttttgt gtcaaataaa ggtctttggc
     2641 gctatgagat ccacgacata gaaggccgcg ttgacccgac actgggaaag cggttaaaag
     2701 cagatgggga tggttggatt gttcctcccg cagctgcgcc gtgggacttc ggtttgattg
     2761 tgctacgtaa tcccccttct ggcattacac cattgccgtt atttgaggga gataaagccg
     2821 cgcttaccgc cgcattaaaa gcggcaggtc gtaaagtgac tcaggcggga taccctgaag
     2881 atcatctcga tacgttgtac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     2941 cggtgatgtc gcatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     3001 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     3061 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     3121 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcatgtt catcaggcaa
     3181 gtttcaccat gatcattcca gccaacagca gcaccaggcc aatccagcct ttacgattta
     3241 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     3301 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     3361 cagccagcac cgccgccaga gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     3421 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     3481 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     3541 cctgatttga tcaacacaat cccggcgacc agggtggtta acccggcaat tttcatcaga
     3601 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     3661 tcccacagcg cataagctac gcctaaggcg atctttttaa cggcgaaaga gagaaatata
     3721 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     3781 gcccatttca tggacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     3841 caataaatat acattgtcct tctcctgcaa gagaattatt ttaattttcg cttaattcag
     3901 cgaaaccaaa gtaaagtggt caagcccgcg cttagcgcaa gttttggcag aagaaaggac
     3961 taaagcgcgt tgcgccagtg ctgctcacct acgagcaaga tagtggatga ggtacgaaga
     4021 tgggtaaatg tagaaaataa cgtcctgaac aaattgtcca taatattaca attatccgca
     4081 gtgttgcttc tcgtcatcgc ggatgataat tgtcctcggt agttgaacac gcctgatttg
     4141 tatcatagct taagaattaa ctcaaaatat tttcacttct ttacctgagc ggtttgattt
     4201 tcgttatgat gacggagcga aaaagacatt attattagca aaggaagaaa aaacggggac
     4261 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     4321 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     4381 cgcattattt attgctgtta ttcttaaccc gctggtgcaa cacatggtcc gctggcgtgt
     4441 gccgcgtgta ctggcagtgt caattttgat gaccatcatc gtgatggcga tggtgttgct
     4501 gttagcttat ctgggttccg cgctcaatga gttgacgcgg acgttacagc aatatcgcaa
     4561 ctctattatg acgccactga aagcgattga accgttgttg caacgcgtag ggattgatgt
     4621 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     4681 cttattgacg cagttatcta atgccatgtc atcaatcttt ttattgctgc tgacggtgct
     4741 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     4801 ggttgaaggg atggcggcga ttcagcgtgc gattgacagt gtgtctcatt atctggtgct
     4861 gaaaacagcc atcagcatca tcaccggcct ggtcgcatgg gcgatgctcg ccgcactcga
     4921 tgttcgcttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tcccgaatat
     4981 tggttcagtt ctcgcggcaa tcccccctat tgctcaggta ctggtgttta atggcttcta
     5041 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     5101 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     5161 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     5221 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     5281 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     5341 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     5401 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     5461 gcgctttcca cacttccagc acaggcatac cagcaatagg actcttcgga tcatcctgcg
     5521 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     5581 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     5641 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacgttga
     5701 taccacgagc gcgcagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     5761 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     5821 cttctgcggt gatttcgcgg tgctcgccca cttcctgatc atcgccagta gaagagccgt
     5881 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     5941 tgtaagaaag gatagcgccc gaagaaccga ccagcgcacc ggtcacaatc agcagatcgt
     6001 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     6061 ccaccactgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     6121 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     6181 atacaatcag cagcaggaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     6241 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgaac
     6301 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     6361 ccagaatcgg tgccattccc gcgtcatgat gcagatagct gttaaagcca accagcactg
     6421 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     6481 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     6541 caacattacc cgtatccggt cccaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     6601 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     6661 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     6721 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     6781 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc gccctggcca
     6841 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtgac cgacatcaac
     6901 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac acaggccagc
     6961 gcaaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgccaa ccagccgaaa
     7021 agaatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     7081 tcggttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     7141 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     7201 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     7261 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     7321 taaccaatca ctttgacacc attttccgta gtgaagattt cacccggcac ggtgtattca
     7381 cagttgccgc cgttttgggc tgccaggtcg acaatcacac tacccgcctt catggagtca
     7441 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     7501 atgatatcga cctcttttgc ctgggcggta aagagttcca tttccgcttt gatgaacgcg
     7561 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctctttaaa atccagctcg
     7621 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     7681 cgcacaatcg cgccgagact gtttgcagcg ccaatggcgg ccagacccgc aacacccgca
     7741 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtaatttg cccggtaaag
     7801 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     7861 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
     7921 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     7981 aaactcacca gcgttgtccc aggattcagt aacgcaattt catcatctaa cggcgcattg
     8041 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     8101 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     8161 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     8221 ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat cggttttatt
     8281 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     8341 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     8401 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     8461 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg gcgttcgcat
     8521 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     8581 agttgcttca gtagtattga tgctgatacg gctgtttttt aagcatagac ggccatttga
     8641 gcaggattaa aattggctta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     8701 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     8761 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     8821 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     8881 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     8941 gccgtttctc gtcgcgctga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     9001 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     9061 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     9121 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     9181 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     9241 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     9301 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     9361 ggacgtgcag ctctggctgc cggtggcgaa gctgcgaaga aagttgagat cccgggtgtt
     9421 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     9481 ggcgggcgtt acactgtcac gctcccagac ggcactaaag tcgaagaact gaacaaagcg
     9541 accgcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     9601 actgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     9661 atcacccgcc agtggcaaga acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     9721 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     9781 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
     9841 aaactccatt tttatgcgtt ttgccctaat atttattctt cttgtcacgt tttaatcact
     9901 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     9961 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
    10021 cactgctcat cggctggagt attactggtg ctggtatttt attactgacc tttgccatgc
    10081 tgatcctcac gcgcattcgt cccgaacttg acggcggtat ctttacctat gcccgcgaag
    10141 gatttggtga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
    10201 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
    10261 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat cgtcggtgca tcggctttat
    10321 tgtggattgt tcactttttg atcctgcgcg gagtgcaaac cgctgccagc attaacctgg
    10381 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
    10441 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cccgtttggg
    10501 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
    10561 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
    10621 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggc gtggtggcac
    10681 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg actgatggtg gaaatgatgg
    10741 gaccgtgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggtgcgtact
    10801 taagctggac catcatggcg gcagaagttc cgttcctggc agccactcat aaagcattcc
    10861 cgcgcatttt tgcgcgccag aacgcacaag ctgcgccatc cgcttcactg tggctgacca
    10921 atatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacat
    10981 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
    11041 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
    11101 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
    11161 cggggctgct ggttttcctg tacgcgcgta aaacgcatac ccatgataat gtactgaatc
    11221 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
    11281 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaagccc
    11341 aacccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcctggc
    11401 atttcattaa tcaaaagcaa ccggtgatta tcagctatcg gacgcactat cccgccattg
    11461 atggactgat taacgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    11521 tgatggcgtt tgccgatgaa gtactaaaaa gcacccatgg cctgcgtgct attttgcata
    11581 acgccagcgc gtggatggca gaaaaaccag gtacaccact gaccgacgtt ctggcttgca
    11641 tgatgcagat ccacgttaat accccatacc tactcaacca tgcactggaa agattactgc
    11701 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    11761 gtagcgacaa acatattgcc tatgctgcaa gcaaagcggc actggataat atgacccgct
    11821 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    11881 tgtttaatga gcatgatgat gccgaatacc gacaacaggc gctgaataaa tcactgatga
    11941 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagctgct
    12001 ttgtcaccgg acgcagtttc cctcttgatg gcggtcgtca tctgcgttaa tgcagcttta
    12061 tccagcatat aatcaacacc catgcgctta tcccccagca tgcaacagaa ccaacaaaag
    12121 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga cgccaggtaa acaaaatagg
    12181 gaataatcga ccacatgcta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    12241 tggcaacaat ataatgcgcg atgagcgcaa aggtcgggaa aagtggtatc agcccggcga
    12301 tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    12361 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag ttatcagtag
    12421 aatacctgat gaaaccttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    12481 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    12541 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgtg
    12601 gaaccgcgcg gcgaccgggc cgaagaaact attctgcaag aaaatccgga tttggtgtta
    12661 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    12721 tggtccggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    12781 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgttagcg
    12841 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgagcaaagg tcttcaggaa
    12901 acgtctctga ctccctacaa agccctgcat ttcggcacgt tgaccatcga tcctatcaac
    12961 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    13021 tgggaattag ctacccatgc tgggcaaatc atggaccgcg atgcattgct gaaaaattta
    13081 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    13141 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgttcg taacaaaggc
    13201 tatctttttg cgcctcatgc atgggaataa gcgatgaaaa aactgtttat ccagttttac
    13261 ctgttattgt ttgtctgctt ccttgtgatg tctctgctgg ttgggctggt gtacaaattt
    13321 accgccgaac gcgcgggcaa acagtcgctg gatgatttga tgaacagttc gctgtatctg
    13381 atgcgcagcg aattgcgtga gatcccccca cacgattggg gtaagacgct gaaggagatg
    13441 gatttaaatc tctctttcga tctgcgagtc gagccactga gtaaatacca tcttgatgat
    13501 atttccatgc accggctgcg tggcggcgaa attgtcgccc tggacgatca gtacactttt
    13561 ttgcaacgta tcccgcgcag ccactacgtg ctggcagttg gtcctgttcc ttatctttat
    13621 tacctccatc agatgcgatt actggatatc gccctgatcg cttttattgc catttccctc
    13681 gcctttccgg tgtttatttg gatgcgtccg cactggcagg atatgttaaa actggaagca
    13741 gcggcgcaac gatttggcga tgggcatctc aatgaacgta tccactttga tgaaggttcg
    13801 agctttgaac gacttggcgt cgcatttaac cagatggcgg acaatatcaa cgccttgatt
    13861 gccagcaaaa aacagcttat tgacggtatc gctcacgaac tgcgaacacc gttagtgcgc
    13921 ctgcgttatc gactggagat gagcgataac ctgagcgccg tcgaatccca ggcgttgaat
    13981 cgcgatatca gtcaacttga agctttaatt gaagagctgc tgacttatgc ccgactcgat
    14041 cgcccacaaa atgagcttca tcttagcgaa ccagacctgc cgttgtggct gtcaacgcat
    14101 ctggcagata ttcaggcagt aacgcccgat aaaacggtac ggataaaaac gctcgcgcaa
    14161 ggccattatg ctgcgttgga tatgcgctta atggagcgcg tgctggataa tttgctcaat
    14221 aacgccctgc gctactgtca ctcaacggtt gaaaccagcc tgctactgtc ggggaataga
    14281 gcgacattaa ttgttgagga tgatggccca gggattgccc ctgaaaaccg cgaacatatc
    14341 tttgaacctt ttgttcgcct cgatcccagc cgggatcgct caaccggcgg ctgcgggctg
    14401 gggctggcaa ttgtccactc tatagcactg gcaatgggcg gtacggttaa ttgtgacacc
    14461 agcgaactgg gtggtgcccg cttctcgttt agctggccgt tatggcataa catcccgcaa
    14521 tttacctctg cttgacatta cgcgcacggt ggtcaagtca ccaccactgt gctataaaat
    14581 aaggatgttg taactaaagt ggttaatatt atggcgcgtt acgatctcgt agaccgactc
    14641 aacactacct ttcgccagat ggaacaagag ctggctgcat ttgccgctga gctagaacag
    14701 cacaaattgc tggttgcccg cgtgttctct ttgccggagg taaaaaaaga ggatgagcat
    14761 aatccgctta atcgtattga ggtaaagcaa catctcggca acgacgcgca gtcgctggcg
    14821 ttacgtcatt tccgccattt atttattcaa caacagtccg aaaatcgcag cagcaagacc
    14881 gctgttcgtc tacctggcgt gttgtgttac caggtcgata acctttcgca agcagcgttg
    14941 gtcagtcata ttcagcacat caatcaactc aagactacgt tcgagcatat cgtcacggtt
    15001 gagtcagaac tccccacagc ggcgcgtttt gaatgggtgc atcgtcattt gccggggctg
    15061 atcaccctta atgcttaccg aacgctcact gttctgcacg atcccgccac tttacgtttt
    15121 ggctgggcca ataaacatat cattaagaat ttgcatcgtg atgaagtcct ggcacagctg
    15181 gaaaaaagcc tgaaatcacc gcgcagtgtc gcaccgtgga cgcgcgagga gtggcaaaga
    15241 aaactggaga gagagtatca ggatattgct accctgccac aggacgcgaa gttaaaaatc
    15301 aaacgtccgg tgaaggtgca gccgattgcc cgcgtctggt acaagggaga tcaaaaacaa
    15361 gtacaacacg cttgccctac accgcttatt gcgctgatta atcgggataa tggcgcgggc
    15421 gtgccggacg ttggggagtt gttaaattac gatgccgaca atgtgcaaca ccgttataaa
    15481 ccacaggcgc agccgcttcg tttgatcatt ccacggctgc acctgtatgt tgcagattaa
    15541 cccccggctt tcatactgcc gaccatctgt tctggccgta cccagctgtc aaactcggct
    15601 tcgctaagat accccagcgc aagggccgca gcttttaagg tcagcccttc tttatgcgct
    15661 tttttggcga tctcggcggc tttgtcataa ccaatgtggg tgttaagcgc agtcaccagc
    15721 atcagcgatt cattgagtaa ttgattgatt cgctcacgat tcggttcaat gcccactgcg
    15781 cagtgtttgt taaaactttc catgccatct gccagcaagc gcaccgattg caggaaatta
    15841 tggatcacca tcggacggaa gacgttcagt tcaaagttac cggaagcgcc acccatgttg
    15901 atcgccacat cgttccccat cacctgacag cagagcatgg ttaatgcttc acactgcgtc
    15961 gggttcactt tccccggcat gattgagcta cccggctcat tttccgggat tgagatttca
    16021 ccaattccgc agcgcgggcc agaggccagc cagcggacat cattggcaat tttcatcagt
    16081 gacgcagcca accctttcaa cgcgccgtgc gcctgaacca gggcatcaca ggtcgccagc
    16141 gcttcaaatt tgttcggcgc ggtaacaaac ggtgcacaag taatgactgc cagttcatct
    16201 gccacgcgac gcgcatactc cggatgggta tttagtccag tacccaccgc tgtaccgcca
    16261 agagccagtt ccgctacatg cggcaggctg tattcgatat gtttcagatt atgctcgagc
    16321 atcgctaccc agccggaaat ctcctgcccc agcgttaacg gcgtggcgtc ctgcaagtgg
    16381 gtacgaccga ttttgacgat atcagcaaat acgcgggatt tttcactcag cgtctgggtc
    16441 agggttttaa gttgtggaat gagttgcttg cgcagcgcca gtagtgccgc aacgtgcatc
    16501 gccgtcggaa agacatcgtt ggaactttgg cttttgttca cgtcgtcgtt agggtgaact
    16561 ttacgttcca tcccgcgcac gccaccgagt aattcactgg cccggttagc cagcacttcg
    16621 ttcatgttca tgttactttg cgtgccggag ccggtctgcc agatagccag cgggaattcg
    16681 tcgtcatgct gtcctgccag tacttcatcc gccgcctgcc gaatggcgct cgctttctct
    16741 tcagacaaca agcctaaatc ttcattaact tttgccgctg cgcgcttggt tagcgccagc
    16801 gcatgaatca gtgaggtggg cattttctcc gtcgaaatgc ggaaatgctc cagcgagcgc
    16861 tgagtttgtg cgccccacag cttatctgcc gggacatcaa tcgctcccat cgaatctttt
    16921 tcgctgcgta ctgtattcat ggcctgctcc tcacctgatt aattttttct ttctgttttg
    16981 ctttcgttaa gcaactttta gcttactcat tatttaccat ttgataacaa atgtttggtc
    17041 tttcgtgcca tgtaaaaaaa ccgccccgaa gggcggctct gtttatttca cacagcgggt
    17101 gcactgtgtg agttgtatct gctggaagaa gtcatttcct ttatcatcca caaggataaa
    17161 tgccgggaaa tcttccactt caattttcca gatggcttcc attcccagtt ccggatattc
    17221 aacacattcc aggctcttaa tgcttccctg cgccaataca gctgccggac caccgatact
    17281 gccaaggtag aagccgccgt gttttttaca ggcatccgtc acctgctggc tgcggttgcc
    17341 tttcgccagc atgatcatac ttccgccctg cgcttgcagt tgatcgacat aagaatccat
    17401 ccgtccggcg gtcgttgggc caagagaacc ggaggcataa ccttccggcg ttttggccgg
    17461 acccgcgtag taaatcggat gatctttaat atactgcggc agcccttcgc cgttatccat
    17521 ccgctctttc agtttggcgt gagcaatatc acgaccaacg ataatcgtgc cattcagcga
    17581 taagcgtgta gaaacgggat actgcgacaa ctgtgcgagg atctctttca tcggacggtt
    17641 aaggtcaacg cgcaccgctt cgccttctcc cgctttacgc agctcttccg ggatatattt
    17701 gcctggatta tgttccagtt tttcgatcca gatcccctga cggttgatct tcgctttgat
    17761 attacggtca gcagagcagg agacgcccat accgaccggg caggatgcgc cgtgacgtgg
    17821 caggcgaatc acgcggatgt cgtgagcgaa gtatttacca ccaaactgcg cacccagacc
    17881 aagattttgc gcttcgatca gcaattcttt ttccagttcc acatcgcgga acgcctgacc
    17941 gtgctcattc ccttccgttg gcagttcgtc atagtattta gcggaagcca gtttcaccgt
    18001 tttaaggttc gtttccgcag aagttccacc aattacgaac gcaatatgat acggaggaca
    18061 ggccgccgta cccagcgtgc gcatcttctc aaccaggtaa tttttcagtt tccccggcgt
    18121 tagtaacgct ttggtttcct gatagagata cgtcttgttt gccgaaccac cacctttggc
    18181 gatacagagg aatttgtact cgtcgccatc aacggcataa agatcgatct gcgctggtag
    18241 attggtgccg gtattcacct ctttatacat atccagcggc gcgttttgcg agtagcgcag
    18301 attatcttcg atataagtgt tatagacacc gcgtgccagc gccgcttcat caccgccacc
    18361 ggtccataca cgctgccctt ttttgccaac aataatcgcg gtgccggtat cctgacaggt
    18421 tggcagaacg cctttcgccg cgatgtcgga gttgcgcagg aattgcagcg ccacatattt
    18481 atcattttcg ctggcttccg ggtcacgcag aatgtcggcc acctgttgtt ggtgagccgg
    18541 acgcagcata aatgacgcat catgaaacgc ctgacgcgcc aacaaagtta acgcttcggg
    18601 tgcgactttc aaaatctcct gcccttcaaa ttcagatacg ctaacgtgtt cgctggttag
    18661 caggtaatac tcagtatcat cttttttgag tggaaaagga gcctgataat gaaagggttt
    18721 gtttgacatt gttctctcac ttactgcctg gtttggttat gctctgggcg ggtgttccgt
    18781 tgccctgtta aaagcgagta acaatatcct acacactttt ttaacaaaaa ctgagactag
    18841 tacgactttt tgcggctcca ggttacttcc cgtaggattc ttgctttaat agcgggatta
    18901 atttccacat taaaacaggg attgatcatg caaaaactca ttaactcagt gcaaaactat
    18961 gcctggggca gcaaaacggc gttgactgaa ctttatggta tggaaaatcc gtccagccag
    19021 ccgatggccg agctgtggat gggcgcacat ccaaaaagca gttcacgagt gcagaatgcc
    19081 gccggagata tcgtttcact gcgtgatgtg attgagagta ataaatcgac tctgctcgga
    19141 gaggccgttg ccaaacgctt tggcgaactg cctttcctgt tcaaagtatt atgcgcagca
    19201 cagccgctct ccattcaggt tcatccaaac aaacgcaatt ctgaaatcgg ttttgccaaa
    19261 gaaaatgccg caggtattcc gatggatgcc gccgagcgta actataaaga tcctaaccac
    19321 aagccggagc tggtttttgc gctgacgcct ttccttgcga tgaacgcgtt tcgtgaattt
    19381 tccgagattg tctccctgct ccagccagtc gcaggtgcac atccggcgat tgctcacttt
    19441 ttacaacagc ctgatgccga acgtttaagc gaactgttcg ccagcctgtt gaatatgcag
    19501 ggtgaagaaa aatcccgcgc gctggcgatt ttaaaatcgg ccctcgacag ccagcagggt
    19561 gaaccatggc aaacgattcg tttaatttct gaatgttacc cggaagacag cggcctgttc
    19621 tccccgctgt tgctgaatgt ggtgaaattg aatcctggcg aagcgatgtt cctgttcgct
    19681 gaaacaccgc acgcttacct gcaaggcgtg gcgctggaag tgatggcgaa ctccgataac
    19741 gtgctgcgtg caggtctgac gcctaaatac attgatattc cggaactggt tgccaacgtg
    19801 aagttcgaag ccaaaccggc taaccagttg ttgacccagc cggtaaaaca aggtgcagaa
    19861 ctggacttcc cgattcctgt ggatgatttt gccttctcgt tgcatgacct tagtgataaa
    19921 gaaaccacca tcagccagca gagtgccgcc attttgttct gcgtcgaagg cgatgcaacg
    19981 ctgtgtaaag gttctcagca gttacagctt aaaccgggtg aatcagcgtt tattgccgcc
    20041 aacgaatcac cggtgactgt cagaggccac ggccgtttag cgcgtgttta caacaagctg
    20101 taagagctta ctgaaaaaat taacatctct tgctaagctt attaaaggct tataacacct
    20161 tcaggcggcc agtccgcctg atttcatttt atggataatc attatgaata aatcgctggt
    20221 agcggtaggc gtcattgttg cgctaggcgt agtctggaca ggcggcgcat ggtatacagg
    20281 caagaagatt gaaacccatc tcgaagacat ggtcgcgcag gcgaacgcgc aactcaaact
    20341 gaccgctcct gaatccaacc tggaagtgag ttatcaaaac tatcatcgcg gcgtattcag
    20401 cagtcagctg caactgttgg tgaaacccat tgccggaaaa gtaaatccgt ggattaaaag
    20461 cggtcagagc gtcatcttca acgaatcggt tgatcatggt cccttcccgc ttgcccagct
    20521 taaaaaactg aatctgatcc cgtcgatggc atcaattcaa accacgctgg ttaataacga
    20581 agtaagcaaa ccactgtttg atatggcaaa aggtgaaacg ccttttgaga ttaactcccg
    20641 cattggttac agcggagatt ccagttccga catttcgctc aagccactga attacgagca
    20701 aaaggatgaa aaagtcgcct ttagcggcgg cgagttccag ttaaatgcag acagagacgg
    20761 caaagctatc tccctttccg gggaggcgca aagtggtcgg atagacgctg ttaacgaata
    20821 caaccagaaa gtacagttga cctttaataa tctgaaaacc gacggttcca gcacgctggc
    20881 aagttttggt gagcgcgtag gaaaccaaaa actgtcactg gaaaaaatga ccatttcagt
    20941 ggaaggcaaa gaactggcac tgctggaagg catggagatc agcggtaaat cggatctggt
    21001 caatgatggt aaaacgatca atagccaact ggattactcg ctaaacagcc tgaaggtaca
    21061 gaatcaggat ctgggcagcg gcaagctgac tttaaaagtc ggccaaattg atggcgaagc
    21121 ctggcatcag tttagccagc aatataacgc gcaaactcag gcgctgctgg cacagccaga
    21181 aattgccaac aatcccgaac tttatcagga aaaagtgacg gaagccttct ttagcgccct
    21241 gccgctgatg ttgaaaggcg atccggtgat tactatcgcg ccgctaagct ggaaaaacag
    21301 tcagggtgaa agtgcgctga atctgtcgct gttcctgaaa gatccggcaa cgactaaaga
    21361 agcgccgcaa acgctggcgc aggaagtaga ccgttcggtt aaatctctgg atgcgaaact
    21421 gaccattccg gtggatatgg caactgagtt gatgactcag gtagcgaagc tggaaggtta
    21481 tcaggaagat caagcgaaaa aactggcgaa acagcaagtt gaaggtgcat cagcaatggg
    21541 gcagatgttc cgtctgacca ccttgcagga caataccatc accaccagcc tgcaatatgc
    21601 taacggtcag atcacgttaa acgggcagaa aatgccactg gaagattttg ttgggatgtt
    21661 tgcgatgccg gcattaaacg ttccggctgt acccgctatt ccgcagcagt aattcacagc
    21721 caaaatgccg gagatttttc tctccggc
//
LOCUS       DAZGAP010000084        21621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGAP010000084 DAZGAP010000000
VERSION     DAZGAP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.084"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1196
                     /locus_tag="ACHLAM_002699"
     CDS             <1..1196
                     /locus_tag="ACHLAM_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7854204.1"
                     /translation="MHSSVEQERQAVISKLIARFEKENPGITVKQVPVEEDAYNTKVI
                     TLSRSGSLPEVIETSHDYAKVMDKEQLIDRKAVATVISNVGEGAFYDGVLRIVRTEDG
                     SAWTGVPVSAWIGGIWYRKDVLAKAGLEEPKNWQQLLDVAQKLNDPANKKYGIALPTA
                     ESVLTEQSFSQFALSNQANVFNAEGKITLDTPEMMQALTYYRNLAANTMPGSNDIMEV
                     KDAFMNGTAPMAIYSTYILPAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITVGQKA
                     EETEAAEKFVTFMEQADNIADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQ
                     LISELPNIQVFGAVGDKNFTRMGDVTGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDE
                     LVEQR"
     gene            1217..2098
                     /locus_tag="ACHLAM_002700"
     CDS             1217..2098
                     /locus_tag="ACHLAM_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7854205.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2085..2927
                     /locus_tag="ACHLAM_002701"
     CDS             2085..2927
                     /locus_tag="ACHLAM_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7854206.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            2958..4010
                     /locus_tag="ACHLAM_002702"
     CDS             2958..4010
                     /locus_tag="ACHLAM_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO7854207.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGSDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4029..4817
                     /locus_tag="ACHLAM_002703"
     CDS             4029..4817
                     /locus_tag="ACHLAM_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7854208.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVITACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEKVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALLEQLRADNFQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            4827..5882
                     /locus_tag="ACHLAM_002704"
     CDS             4827..5882
                     /locus_tag="ACHLAM_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7854209.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            5879..8146
                     /gene="ycjT"
                     /locus_tag="ACHLAM_002705"
     CDS             5879..8146
                     /gene="ycjT"
                     /locus_tag="ACHLAM_002705"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO7854210.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWCTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLGTAK
                     FWISRAVQVNDRLEIHNVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARKFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            8143..8802
                     /gene="pgmB"
                     /locus_tag="ACHLAM_002706"
     CDS             8143..8802
                     /gene="pgmB"
                     /locus_tag="ACHLAM_002706"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO7854211.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            8816..9898
                     /locus_tag="ACHLAM_002707"
     CDS             8816..9898
                     /locus_tag="ACHLAM_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7854212.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFLNYALYPHMTVYDNMAFGLK
                     MQKIANEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTISEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            9943..10848
                     /gene="ompG"
                     /locus_tag="ACHLAM_002708"
     CDS             9943..10848
                     /gene="ompG"
                     /locus_tag="ACHLAM_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO7854213.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10899..11897)
                     /locus_tag="ACHLAM_002709"
     CDS             complement(10899..11897)
                     /locus_tag="ACHLAM_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7854214.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGNQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            12052..13449
                     /locus_tag="ACHLAM_002710"
     CDS             12052..13449
                     /locus_tag="ACHLAM_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO7854215.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13446..14507
                     /locus_tag="ACHLAM_002711"
     CDS             13446..14507
                     /locus_tag="ACHLAM_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO7854216.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14655..16196
                     /gene="tyrR"
                     /locus_tag="ACHLAM_002712"
     CDS             14655..16196
                     /gene="tyrR"
                     /locus_tag="ACHLAM_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO7854217.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16240..16746)
                     /gene="tpx"
                     /locus_tag="ACHLAM_002713"
     CDS             complement(16240..16746)
                     /gene="tpx"
                     /locus_tag="ACHLAM_002713"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO7854218.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            16865..17830
                     /gene="ycjG"
                     /locus_tag="ACHLAM_002714"
     CDS             16865..17830
                     /gene="ycjG"
                     /locus_tag="ACHLAM_002714"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO7854219.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(17805..18533)
                     /gene="mpaA"
                     /locus_tag="ACHLAM_002715"
     CDS             complement(17805..18533)
                     /gene="mpaA"
                     /locus_tag="ACHLAM_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO7854220.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(18824..19462)
                     /locus_tag="ACHLAM_002716"
     CDS             complement(18824..19462)
                     /locus_tag="ACHLAM_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7854221.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(19533..20453)
                     /locus_tag="ACHLAM_002717"
     CDS             complement(19533..20453)
                     /locus_tag="ACHLAM_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7854222.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            20591..21490
                     /gene="pgrR"
                     /locus_tag="ACHLAM_002718"
     CDS             20591..21490
                     /gene="pgrR"
                     /locus_tag="ACHLAM_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO7854223.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT         5169 a         5245 c         5872 g         5335 t
ORIGIN      
        1 ttatgcattc ttcggtggag caggagcgcc aggccgtcat cagcaaattg attgcccgtt
       61 ttgaaaaaga aaacccaggt atcaccgtaa aacaagtgcc cgtggaagaa gacgcctata
      121 acaccaaagt cattaccctt tcacgtagcg gttcgctgcc ggaagtgatc gaaaccagcc
      181 atgactacgc caaagtgatg gacaaagagc agcttatcga tcgtaaagcg gttgccacgg
      241 tcatcagcaa cgtcggtgaa ggcgcgttct acgatggcgt actgcggatt gtgcgcaccg
      301 aagatggcag cgcctggacc ggtgttcctg tcagcgcatg gattggcggg atctggtatc
      361 gcaaagatgt gctggcaaaa gctgggctgg aggagccaaa aaactggcag caattgctgg
      421 acgttgcaca gaaactgaat gacccggcga ataaaaaata cggtattgcg ctgcctacag
      481 cagaaagcgt gttgacggaa cagtctttct cccagtttgc gttatccaat caggctaacg
      541 tctttaacgc cgaaggcaaa atcacccttg atacaccaga gatgatgcag gcgctgacct
      601 attaccgcaa ccttgctgcc aacaccatgc caggttctaa cgacatcatg gaagtgaaag
      661 acgcttttat gaacggcacc gcgccgatgg cgatttactc cacctatatc cttccggctg
      721 tgattaaaga aggcgatccg aaaaacgttg gtttcgttgt acccaccgag aaaaactctg
      781 cggtctacgg catgttgacc tcgctgacca ttaccgtcgg gcaaaaggcc gaagagacgg
      841 aagcggctga aaaatttgtc acctttatgg agcaggcaga caacattgcc gactgggtga
      901 tgatgtcgcc aggcgcggcg ctgccggtga acaaagcggt tgtcactacc gccacctgga
      961 aagacaacga cgttattaag gcgctgggtg agctgccaaa tcagttaatc agcgaactgc
     1021 caaatattca ggtctttggc gcagtagggg ataaaaattt tacccgcatg ggcgatgtga
     1081 cgggttctgg cgtggtgagc tccatggtgc ataacgtcac cgtgggtaaa gccgacctcc
     1141 ctggcacgct gcaaacgagc cagaaaaagc tggatgagtt ggttgaacag cgctaaaccc
     1201 aggacaggaa tccgcaatga acaggctttt ttcaggtcgt tccgatatgc cctttgcgct
     1261 gttgcttctc gcgcccagct tattactgct gggcggtctg gtggcgtggc cgatggtgtc
     1321 gaatatcgaa atcagttttt tacgtctgcc gcttaatccc aacatcgagt caacgtttgt
     1381 tggggtgagc aactacgtgc gtattctctc cgatcccggc ttctggcact cgctgtggat
     1441 gacggtctgg tataccgcgc tggtggtggc gggcagcacc gtgctcgggc tggccgtggc
     1501 gatgtttttc aaccgcgagt tccgcctgcg caaaaccgcg cgttcgctgg tgatcctctc
     1561 ctacgtaacg ccgtctattt cgctggtgtt cgcctggaaa tacatgttca acaacggtta
     1621 cggcattgtg aactacctcg gcgtcgatct tctgcatctc tatgagcagg caccgctgtg
     1681 gttcgataat ccgggcagta gctttgtgct ggtggtgctg ttcgccatct ggcgctactt
     1741 cccctatgcc tttatttcgt ttctggcgat tttgcagacc atcgacaaat cgctgtacga
     1801 agcggcagag atggatggcg ctaatgcctg gcaacggttt cgtatcgtca cgctgcccgc
     1861 gattatgccg gtcctggcga cggtggtgac cctgcgcacc atctggatgt tctacatgtt
     1921 cgcggatgtt tatctgctga cgaccaaggt cgatattctc ggtgtctatc tctacaaaac
     1981 cgcctttgcc tttaatgact tagggaaagc ggcggcgatc tcggtggtgc tcttcatcat
     2041 tattttcgct gtcattctgc tgaccagaaa acgggtgaac ctcaatggca acaaataaac
     2101 gcacactcag tcgcatcggt ttttactgcg ggctggcgct gtttctcatc atcacgctgt
     2161 ttccgttttt tgtgatgctg atgacctcgt tcaagagcgc aaaagaggcg atctcgctgc
     2221 atcctacgct gctgccgcaa cagtggacgc tggagcatta tgtcgacatt tttaacccga
     2281 tgattttccc gtttgtcgac tacttccgta acagcatggt ggtgtcggtg gtttcatccg
     2341 tggtggcggt atttctcggc attctcgggg cttacgcgct ttcccgcctg cgctttaaag
     2401 ggcggatgac catcaacgcc agcttttaca cggtgtatat gttctccggc attttgctgg
     2461 tggtgccgct gttcaaaatc atcaccgcgc ttggcatata cgacaccgag atggcgctga
     2521 tcattaccat ggtaacgcaa accctgccca ccgccgtgtt tatgctgaaa agctacttcg
     2581 acaccatccc tgatgagatc gaagaagcgg cgatgatgga tggcctcaac cgtctgcaaa
     2641 tcatcttccg tatcaccgtg cccctggcga tgtccgggct gatatccgta ttcgtctact
     2701 gctttatggt ggcatggaac gactatctgt ttgcgtcgat tttcctctcc agcgccagca
     2761 atttcacctt accagtcggc ctgaacgcgc tgttcagtac gcctgattac atctggggac
     2821 ggatgatggc ggcctcgctg gtgaccgcat tgccggtggt gattatgtat gcgctttcgg
     2881 agcgttttat taagagtggt ttgaccgccg gtggcgtgaa gggctaaagc ggccagtttc
     2941 tttacaagga gttttacatg aaaaagttag tagccacagc accgcgcgta gctgcgctgg
     3001 ttgagtatga agatcgggcg attttagcca atgaagtgaa gatccgcgtg cgcttcggcg
     3061 caccgaagca cgggacggaa gtggtcgatt tccgcgctgc cagtccgttt atcgatgaag
     3121 actttaacgg cgaatggcag atgttcaccc cgcgtccggc agatgcgccg cgcggcattg
     3181 agtttggcaa attccagctt ggcaacatgg tggttggcga cattatcgag tgcggcagcg
     3241 acgttaccga ctacgcggtg ggcgacagcg tatgcggcta cggcccgctc tccgagacgg
     3301 tcatcattaa cgcagtgaat aactacaagc tgcgcaaaat gccgcaaggc agttcgtgga
     3361 aaaacgctgt ctgctacgac ccggcgcagt ttgccatgag cggcgtgcgc gatgccaatg
     3421 tgcgcgtggg cgattttgtg gtggtggtag ggcttggcgc gatcggtcaa attgccattc
     3481 aactggctaa acgtgctggc gcgtcggtgg tgattggcgt cgatcctatc gcccatcgct
     3541 gtgagattgc ccgtcgccat ggcgcggatt tctgccttaa ccccattggc agtgacgtag
     3601 gcaaagagat caaaacgcta accggcaagc agggtgccga tgtgattatc gaaaccagcg
     3661 gctatgccga cgcgctgcaa tcggcgctac gaggtctggc atatggcggc accatctcgt
     3721 atgtcgcgtt tgccaaaccg tttgccgaag gttttaacct cggacgcgaa gcccatttca
     3781 ataacgccaa aattgttttc tcccgtgcgt gcagcgagcc gaacccggat tatccgcgct
     3841 ggagccgcaa acgtatcgaa gaaacctgtt gggaactgct gatgaacggt tatctcaatt
     3901 gtgaagattt aatcgacccg gtggtgacct ttgccaatag cccggaaagt tacatgcagt
     3961 atgtcgacca gcatccggaa cagagcatca aaatgggcgt tacgttttaa ttcaaaggaa
     4021 tgaaaaaaat gaaaatcggc acacagaatc aggcgttctt tccggaaaac attctggaga
     4081 aatttcgtta tatcaaagag atgggtttcg atggttttga gatcgacggc aaactgctgg
     4141 tcaacaatct cgaagaagtc aaagcagcaa tcaaagaaac cggtttaccg gtgatcaccg
     4201 cctgcggtgg ctatgacggt tggattggcg actttatcga agagcgtcga cttaatggct
     4261 taaagcagat cgaacgcatt cttgaagctc tggcagaagt gggcggtaaa ggtatcgtcg
     4321 ttccggctgc atggggcatg tttaccttcc gcttaccgcc gatgacctcg ccgcgtagcc
     4381 tggacggcga ccgcaaaatg gtgagtgatt ccctgcgcgt actggaaaag gtggccgcac
     4441 gtaccggaac cgtggtgtac ctcgaaccgt taaaccgtta tcaggatcac atgatcaaca
     4501 ctctcgccga tgcccgccgt tatatcgtcg aaaacgatct taaacatgta cagattatcg
     4561 gcgatttcta tcacatgaat atcgaagaag ataacctggc gcaggcgctg catgacaacc
     4621 gcgacctgct cggtcatgtg catattgccg ataaccatcg ctaccagccg ggcagcggca
     4681 ccctggattt ccacgcgctg ttagaacagc tgcgcgcgga taactttcag ggctatgtgg
     4741 tgtacgaagg gcgcatccgg gcggaagatc cagcccaggc gtaccgtgat tctttggcct
     4801 ggttgcgtac ctgctaagag gtctttgtga aaagtgcaat gacaagctct ccgctgcggg
     4861 tcgcgataat aggcgcgggc caggtggcgg ataaagttca tgcttcgtac tactgcaccc
     4921 gcaacgatct ggaactggtg gctgtctgtg acagccgcct ttcccaggcg caggcgctgg
     4981 cagaaaaata cgggaatgca tccgtgtggg acgatccgca ggccatgctg ctggcggtga
     5041 aacctgatgt ggttagcgtc tgctcaccta accgttttca ttacgaacat accctgatgg
     5101 cgctggaagc gggctgccat gtgatgtgcg aaaaaccgcc cgccatgacg gcagaacagg
     5161 cgcgggaaat gtgcgatacc gcgcgcaaac agggcaaggt gctggcctac gactttcacc
     5221 atcgttttgc actcgatacg caacagctgc gtgaacaggt gaccaacggc gttttgggag
     5281 agatttacgt taccaccgcc cgcgccctgc gtcgttgcgg tgttcccggc tggggtgtct
     5341 ttactaataa agaattgcag ggcggaggcc cgctgatcga catcggcatt catatgctgg
     5401 atgcggcgat gtatgtgctg ggttttccag cggtgaaaag cgtgactgcg catagctttc
     5461 aaaagatcgg cacgcaaaag agctgcggtc aatttggcga gtgggacccg gcaacctaca
     5521 gcgtcgaaga ttcgctgttt ggcaccattg aatttcataa cggcggcatt ctgtggcttg
     5581 agacctcatt tgcactcaac atccgcgaac agtcgattat gaacgtcagc ttttgtggtg
     5641 ataaagccgg ggcgacgctg tttccagcac atatctacac cgataacaac ggtgagttaa
     5701 tgacgctgat gcaacgggaa atggcagacg acaaccgtca tctgcgcagt atggaagcct
     5761 ttatcaacca cgtacaaggt aagcccgtga tgatagccga cgccgagcag ggatacatca
     5821 tccagcaact ggtggcggcg ctttatcaat ccgcagaaac agggacgcgt gtggaattat
     5881 gatcaggcca gtaacgttaa cggaacccca tttcagccag cataccctga acaagtatgc
     5941 ttcgctgatg gcgcagggga acggctatct tgggcttcgc gccagccatg aagaagatta
     6001 cacgcgccag acgcgcggca tgtatctggc agggctttat catcgggcgg gtaaaggtga
     6061 aatcaacgaa ctggtgaacc tgcctgatgt cgtggggatg gagattgcca taaatggtga
     6121 gattttctcg ttatcccgcg aagcatggca gcgtgagctt aactttgcca gtggcgaatt
     6181 acgccgcaac gttgtctggt gtaccagcaa cggcgcaggt tacaccatca ccagccgtcg
     6241 ctttgtttcg gcagaccaac tgccgctcat tgcgctggaa atcactatta cgccactgga
     6301 cgccgacgcg ttagtgctga tttcaacagg cattgacgcc acgcaaacca accacggtcg
     6361 ccaacatctc gacgaaaccc aggtgcgggt atttggtcag catctgatgc aggggatcta
     6421 caccacccag gatgggcgca gtgatgtggc catcagctgt tgctgtaagg tgagcggtga
     6481 tgtgcagcaa tgctataccg ccaaagagcg ccgtttactg caacatacct gcgcgcagct
     6541 tcacgcaggt gagacgctga cgctgcaaaa acgggtgtgg atcgactggc gggacgacag
     6601 acaagccgct ttagacgaat ggggcagtgc gtcgcttcgt cagcttgaaa tgtgcgcgca
     6661 gcagagttac gaccaacttc ttgcagcatc aacagaaaac tggcgtcaat ggtggcagaa
     6721 acgtcgtatc acggtaaacg gcggcgatgc gcacgatcag caagcgttag attatgcgct
     6781 ttatcacctg cgcatcatga cgcctgccca cgatgagcgc agcagcattg cggcgaaagg
     6841 tttaaccggc gaaggctaca aaggccacgt tttctgggat acagaagtat ttttgctacc
     6901 gttccatctg tttagcgatc cgacggttgc ccgaagttta ctgcgttatc gctggcacaa
     6961 cttgcctggc gcgcaggaga aagcgcgacg caacggctgg cagggcgctc tgtttccgtg
     7021 ggaaagcgcg cgcagcggcg aagaagagac gccggaattt gccgccatta acatccgcac
     7081 cggactgcgc cagaaagtgg cctcggcgca ggctgaacac catttggtgg ctgatatcgc
     7141 ctgggcggtt attcaatact ggcaggcaac gggggatgaa agtttcattg cccacgaagg
     7201 catggcgtta cttctgggaa ccgcaaaatt ctggattagc cgcgctgtac aggtaaacga
     7261 tcgtctggaa attcacaatg ttattgggcc agacgaatat accgaacatg tcaataataa
     7321 cgccttcacc agctatatgg cgtattacaa cgtccagcag gcgctgaata tcgctcgtaa
     7381 gtttggttgt agcgacgacg cgtttattca tcgtgccgaa atattcctca aagaactgcg
     7441 gctgccagaa attcagcctg acggcgtttt gccgcaggat gattcgttta tggcgaaacc
     7501 agcgattaat ctggcgaaat acaaagcggc ggcggggaag caaaccattc tgctggatta
     7561 ttcacgcgca gaagtgaacg agatgcagat cctcaaacaa gctgatgtgg tgatgctcaa
     7621 ttacatgctg ccggagcagt actcagcggc atcgtgtctt gccaatctgc aattttatga
     7681 accgcgcacc attcacgact cttcactgag taaagcgatc cacggcattg ttgccgcacg
     7741 ctgtggcctg ttggcgcaaa gttatcagtt ctggcgcgag gggactgaaa tcgatcttgg
     7801 tgctgatccg catagctgtg atgacggtat ccacgcagcc gcaactggcg ctatttggct
     7861 gggggcgatt cagggttttg ccggggtgag cgtgcgtaac ggtgaattgc atctcaatcc
     7921 agcgttacct gagcagtggc aacagttgtc tttccctctg ttctggcagg gctgcgaatt
     7981 acaggtcact ctcgacgcgc agcgtattgc gattcgaact tctgcgcccg tttcactgcg
     8041 tttgaacggg cagcttatat ctgtggctga agaatctgtt ttctgtttgg gtgattttat
     8101 tttgcccttc aatgggaccg ctaccacgca tcaggaggat gaatgaaact gcaaggggta
     8161 attttcgatc tggatggtgt aatcaccgat accgcgcatc tgcatttcaa ggcgtggcag
     8221 cagattgccg ctgaaattgg catcagcatt gatgcgcagt ttaacgaatc cctaaaaggg
     8281 atcagccgcg atgagtctct gcggcgcatt ctgcaacacg ggggcaaaga gggcgacttt
     8341 aacccgcagg agagggcgca actggcgtat cgcaaaaatc tgctctacgt ccactcactg
     8401 cgcgaactga cggttaatgc tgtgctgccc ggcattcgca atttgctggc agatctccgt
     8461 gcacagcaga tccctatagg gctggcttct gtctccctga atgcgccgac gattttagca
     8521 gcgctggagc tgcgcgagtt tttcaccttc tgcgcggacg cttcccaact taaaaactcg
     8581 aaaccggacc cggaaatctt tctcgccgcc tgtgccggac tgggcgtgcc gccgcaggca
     8641 tgtatcggca ttgaagatgc gcaggcgggc attgacgcca ttaacgccag cggtatgcgc
     8701 tcggtgggga tcggcgcggg cttaaccggg gcgcaattat tgttgccctc aacggactcc
     8761 ctgacctggc cgcggttatc ggccttctgg caaaacgtat agcaaaggaa tcaacatggc
     8821 tcagctttcg ttacaacata ttcaaaaaat ctacgataac caggtgcatg tggtgaagga
     8881 cttcaacctg gaaattgccg ataaagagtt cattgtcttt gtcggtccgt caggctgcgg
     8941 taaatcgact acgctgcgca tgattgccgg gcttgaggag atcagcggcg gcgatctgtt
     9001 gatcgacggc aaacgaatga atgacgttcc ggctaaagag cgcaatatcg cgatggtgtt
     9061 cctgaactac gcgctgtatc cgcatatgac ggtctacgac aacatggcgt ttggcctgaa
     9121 gatgcaaaaa atcgccaacg aggtgattga tgagcgggtg aactgggcgg cgcaaattct
     9181 cggcctgcgt gagtacctga aacgcaagcc gggggcgctt tccggcgggc aacgtcagcg
     9241 agtggcgctc gggcgggcga ttgtgcgcga agcgggcgtg tttttaatgg atgaaccgct
     9301 ctctaacctg gatgccaagc tgcgcgtgca aatgcgcgca gagatcagca agctgcatca
     9361 gaaactgaac accaccatga tctacgtgac ccacgatcag accgaagcga tgaccatggc
     9421 gacgcggatt gtgattatga aggatgggat tgttcagcaa gtaggtgcgc cgaaaacggt
     9481 ttataaccaa ccggcgaata tgtttgttgc cggatttata ggatcaccgg cgatgaattt
     9541 tattcgcggc acgattgatg gcgataaatt cgttacggaa acgcttaaat taaccatttc
     9601 cgaggagaaa ttagcggttc tgaaaacaca ggaaagtttg cataagccca ttgtgatggg
     9661 aatacgcccg gaagatattc atccggacgc gcaagaggaa aataacattt ccgccaaaat
     9721 tagcgtggca gaattaaccg gtgctgaatt tatgctctac accacggttg gggggcacga
     9781 actggtggtc cgtgctggtg cgttaaatga ttatcatgca ggagaaaata tcactattca
     9841 ttttgatatg actaaatgtc atttctttga tgcagaaacg gaaatagcaa ttcgctaaat
     9901 acagggggaa ggcattcccc caggataata caaggaacaa taatgaaaaa attattaccc
     9961 tgtaccgcac tggtgatgtg tgcgggaatg gcctgcgcac aggccgagga aaagaacgac
    10021 tggcacttta atatcggcgc gatgtacgaa atagaaaacg tcgagggtta tggcgaagat
    10081 atggatgggc tggcggagcc ttctgtctat tttaatgccg ccaacgggcc gtggagaatt
    10141 tctctggcct attatcagga agggccggta gattatagcg cgggtaaacg tggaacgtgg
    10201 tttgatcgcc cggagctgga ggtacattat cagttcctcg aaagcgatga tttcagtttc
    10261 ggtctgactg gtggtttccg taattatggt tatcactacg ttgatgaacc gggtaaagac
    10321 acggcgaata tgcagcgctg gaaaattgcg ccagactggg atgtgaaact gactgacgat
    10381 ttacgtttca acggttggct gtcgatgtat aaatttgcca acgatctgaa tacgaccggt
    10441 tatgctgata cccgtgtcga aacggaaacg ggtctgcaat ataccttcaa cgaaacggtt
    10501 gccttgcgag tgaactatta tctcgagcgc ggtttcaata tggatgatag ccgcaataac
    10561 ggtgagtttt ccacgcaaga aattcgcgcc tatttgccgc tgacgctcgg caaccactcg
    10621 gtgacgccgt atacgcgcat tgggctggat cgctggagta actgggactg gcaggatgat
    10681 attgaacgtg aaggccatga ttttaaccgt gtggggttat tttacggtta tgatttccag
    10741 aatggtcttt ccgtttcgct ggaatacgcg tttgagtggc aggatcacga cgaaggcgac
    10801 agcgataaat tccattatgc aggtgtcggc gtaaattact cgttctgata atgggttaaa
    10861 ttgccggatg cggcgcgagc gccaaatccg gcatacattt atttcaacga catatgcgtg
    10921 gcaacggtaa tattttgtgg tgacggtttt ccggcaatta agcggaataa taactcgcag
    10981 ctttgttgcc ctaactcctg cgtcggaaca tcgatgccgc ccggtgcagg cgttaaaata
    11041 aatgacagcg tttcattgct atagcccacc accgctaact gctgcggaat agcaatattt
    11101 ttctctgctg ccgcacgata aatgctcatt aatttcaggc tgtcagtggc aaatacggct
    11161 tctggcaacg gcgactggct taataattgc cgtgcggctt gcaaggctgt ttcatgggta
    11221 taaccgccgt caacaatcca ttcatcacgc actgcaatat tatgcgcagc cagactttgc
    11281 ttatagccat taacgcggtc aaccgagaca tggacatcaa gcggagcatg caggcaggca
    11341 atattttgat tcccactttc aattaacgca tcggtcaacg caatgctgtc gccaaaatta
    11401 tcggtatcga cagaataaac atgggcatat tgaccttcaa ctttgccaat taccaccacc
    11461 ggaatatcat atttatcgag ttgggcaaaa aatgactcat ccgctggcga acttagcata
    11521 ataatgcctt taatcatttt ctgcttaatt ttgctttcgc atttttgtaa gtcttctgcc
    11581 aggttgtgcg acgtctgcaa tatcacgtcg aaaccttctt cttcagcttt ggcggtgatg
    11641 gcatgtaaaa cttccgagaa aaacggatta cccgccgtag ttttggtcga acgggtagaa
    11701 atcaccataa tggcatcaaa acccgaagag gtcagcgcgc gtgccagctt gtttggttga
    11761 tactgtaatt cttcaatggc ccgtaacact ttttcgcgcg cttccgggga gatattggtt
    11821 tgcttattca gcacgcgtga tacggtggat tttgatacgc ctgcaaccct ggcaatatca
    11881 taaatagtag gggacatagg tcaggaactc cgtccgaacg tgaatgttgc acatcttatg
    11941 gagttcgccc gtcgatgaca acacgggcag ttgataatca atggcctggc ccccacattc
    12001 atatccttac gaatgatttt tttctggtct tcagagcagc acaggacagc aatgaagcga
    12061 cttaaaaatg aacttaatgc gctggtgaat cggggtgtcg acagacatct gcgcctcgct
    12121 gtaaccggac ttagccgcag cggcaaaaca gcgtttatca ccgcgatggt taatcagttg
    12181 ctcaatattc atgccggagc acgtttgccg ctattaagtg cggtgcgtga agaacgcctg
    12241 ctgggcgtaa aacgcattcc ccagcgtgac tttggcattc cgcgcttcac ctatgatgaa
    12301 ggactggcgc agttatatgg cgatcctccc gcctggccga cgccaacgcg cggcgtcagc
    12361 gaaattcgcc tggcgctacg ctataaatcg aacgattcgc tgctgcgcca ctttaaggat
    12421 acctccacgc tgtatttgga gattgtggat taccccggcg aatggttgct cgacctgccg
    12481 atgctggcgc aggactattt aagctggtcg cgccagatga cgggcttact caatggtcag
    12541 cgcggcgaat ggtcggcgaa atggcgaatg atgtgcgaag ggctggaccc gctagcgcct
    12601 gccgacgaaa accggctggc agacattgcc gccgcgtgga ccgattatct ccaccactgt
    12661 aaacagcagg ggctgcactt tattcagcct gggcgctttg tcttgccggg agatatggca
    12721 ggtgcgcccg cgctgcaatt cttcccatgg ccagatgtcg atgcctgggg cgagtctaaa
    12781 ctggcgcagg ccgataaaca caccaacgcc ggaatgttgc gcgagcgttt taattattac
    12841 tgcgagaagg tggtgaaagg attctataaa aatcacttcc tgcgttttga ccgccagatt
    12901 gtgctggtgg actgcctgca accgctcaac agtggaccac aggcatttaa tgatatgcgt
    12961 ctggcgctga cgcagctgat gcaaagtttc cactacgggc agcgtaccct gttccggcgt
    13021 ttgttttcgc cggttatcga taagctattg tttgctgcca ctaaagcgga ccatgtgacc
    13081 atcgatcagc acgctaatat ggtttcatta ctgcaacaac tgattcagga tgcctggcaa
    13141 aatgcagcgt tcgaagggat cagcatggac tgcctggggc tggcgtcagt tcaggcgacc
    13201 accagcggca ttattgatgt taacggtgag aaaatcccgg cgctgcgcgg taatcgactt
    13261 agtgatggcg caccgctcac tgtttatcct ggcgaagtcc ccgcacgttt gcctggtcag
    13321 gcgttctggg ataagcaagg cttccagttt gaggcgtttc gtccgcaggt gatggatgtc
    13381 gacaaaccgc taccgcatat tcgccttgat gctgcgctgg aatttttaat aggagataaa
    13441 ttgcgatgac cgaaccgtta aaaccacgta ttgatttcga cggtccactg gaggtcgatc
    13501 agaatccaaa attcagggcg cagcagacct ttgacgaaaa tcaggcgcaa aattttgccc
    13561 cggccacgct cgacgaagcg caggaagaag aggggcaagt tgaagcggtg atggacgcag
    13621 cgttacgtcc gaaacgcagc ctgtggcgca aaatggtgat gggcgggttg gctctgtttg
    13681 gcgcaagcgt tgtcgggcag ggtgtacagt ggacaatgaa cgcctggcaa acccaggact
    13741 gggtggcgct gggtggatgt gccgctgggg cattgattat cggcgctggc gtaggttctg
    13801 tggtaacaga gtggcggcgc ttatggcgct tgcgacagcg cgcccatgaa cgcgacgaag
    13861 cgcgtgattt attgcatagt cacggcacgg gtaaaggccg cgcattttgc gaaaaactgg
    13921 cacagcaggc gggcatcgat cagtcacatc cggcgctgca acgctggtat gcctcaatcc
    13981 atgaaacgca aaacgaccgt gaagtggtca gtttgtatgc gcatttggtc cagccagttt
    14041 tagatgccca ggcgcggcgc gaaatcagcc gttcggcagc ggaatcaaca ttgatgattg
    14101 cggtcagccc actggcgctg gtggatatgg cttttatcgc ctggcgcaat ctgcgtttga
    14161 tcaatcgcat cgccacgctg tatggcattg aactgggata ttacagccgt ttgcgcctgt
    14221 ttaagctggt attgctgaat atcgctttcg ccggagccag cgaactggtg cgcgaagtgg
    14281 ggatggactg gatgtcgcaa gatctcgctg cgcgtttgtc tacccgggca gctcagggga
    14341 ttggtgctgg acttctgacg gcacgactgg ggattaaagc tatggaactt tgccgcccgc
    14401 tgccgtggat tgaagatgac aaacctcgcc tcggggattt ccgtcgtcag cttatcggtc
    14461 aggtgaaaga gacgctgcaa aagggcaaaa cgcccagcga aaaataatgc aatatcgggt
    14521 gctgaccgga tatctttacg ccgaagtgcc cgtttttccg tctttgtgtc aatcattgtt
    14581 gacagaaacc ttcctgctat ccaaatagtg tcatatcatc atattaattg ttcttttttc
    14641 aggtgaaggt tcccatgcgt ctggaagtct tttgtgaaga ccgacttggt ctgacccgcg
    14701 aattactcga tctactggtg ctgagaggca ttgatttacg cggtattgag attgacccca
    14761 ttgggcgaat ctacctcaat tttgccgaac tggaatttga aagtttcagc agtctgatgg
    14821 ccgaaatacg ccgtattgca ggtgttaccg atgtgcgcac tgttccgtgg atgccttccg
    14881 aacgtgagca tctggcgttg agcgcgttac tggaggcgtt gcctgagcct gtgctttctg
    14941 ttgatatgaa aagcaaagtg gatatggcga acccggcgag ctgtcagctt tttgggcaaa
    15001 aattggatcg cctgcgcaac cataccgccg cgcaattaat taacggcttt aattttttac
    15061 gctggctgga aagcgaaccg caagactcgc ataacgagca tgttgttatt aatgggcaga
    15121 atttcctgat ggagattacg cctgtttatc ttcaggatga aaatgatcaa catgttctga
    15181 ccggtgcggt ggtgatgttg cgctcgacga ttcgtatggg ccgtcagttg cagaatgtcg
    15241 ctgcacagga cgtcagtgcc ttcagtcaaa ttgtcgccgt cagcccgaaa atgaagcatg
    15301 ttgtcgaaca ggcgcagaaa ctggcgatgc taagcgcgcc gttgctgatt acgggtgaca
    15361 ccggtacagg taaagatctc tttgcctacg cctgccatca ggcaagccca cgagcgggca
    15421 aaccttacct ggcgctgaac tgcgcgtcta taccggaaga tgcggtcgag agtgaactgt
    15481 ttggtcatgc tccggaaggt aaaaaagggt tctttgagca ggcgaacggt ggttcggtgc
    15541 tgttagatga gataggcgag atgtcgccaa ggatgcaggc gaaattactg cgtttcctca
    15601 atgatggtac tttccgtcga gttggtgaag atcatgaagt gcatgtcgat gtaagggtga
    15661 tttgcgctac gcagaagaat ttggtcgaac tggtgcaaaa aggcgtgttc cgtgaagatc
    15721 tctattatcg tctgaacgtg ttgacgctca atctgccgcc gctacgtgac tgtccgcagg
    15781 acatcatgcc gttaaccgag ctgttcgtcg cccgctttgc cgacgagcag ggcgtgccgc
    15841 gtccgaaact ggccgctgac ctgaatactg tacttacacg ttatgcgtgg ccgggaaatg
    15901 tgcggcagtt aaagaacgct atctatcgcg cactgacaca actggacggt tatgagctgc
    15961 gtccacagga tattttgttg ccggattatg acgccgcaac ggtagccgtg ggcgaagatg
    16021 cgatggaagg ttcgctggac gaaatcacca gtcgttttga acgctcggta ttaacccagc
    16081 tttatcgcaa ttatcccagc acgcgcaaac tggcaaaacg tctcggcgtt tcacataccg
    16141 cgattgccaa taagttgcgg gaatacggtc tgagtcagaa gaagaacgaa gagtaagcgc
    16201 gaatatgcct gatggtgcaa caccatcagg catattaact tatgctttca gtacagccag
    16261 agctgcttcg taatccggct cggtggtgat ttcatccacc agctggctga aaatcacatt
    16321 gtcattttca tcaataacca caacggcacg cgctgccaga cctttcagtg ggccatcagc
    16381 aattgccaca ccgtaggctt gcagaaattc agcgttacgg aaagtggaga gggtgataac
    16441 gttgttcaga ccttctgcgc cgcagaaacg agactgggcg aacggcagat cggcagagat
    16501 acacagcaca acggtgttgt cgatctctgt tgccagttgg ttaaacttac gtactgatgc
    16561 ggcgcaaaca ccggtatcaa tactcgggaa aatgttcagc actttgcgtt tacccgcaaa
    16621 ctgaccgagg gtgacgtcag acagatcttt tgccacgaga gtaaaagtct gcgctttgct
    16681 acccgcctgc gggatggaat tggcgactgt aaccgggttg ccctggaaat gaacggtttg
    16741 tgacatgatt atctttcctg tttacatata gttaacgtca cacctagttt atgctaactg
    16801 tcaataacac agcaaacgct atttgcgctt aatccgcaga ccaccgcgac aacaaggagt
    16861 aaagatgaga accgttaagg tattcgagga aggctggccc ttacataccc cgtttgtgat
    16921 tgctcgggga agtcgcagtg aagcgcgcgt ggtggtggtt gaactggaag aagaaggtat
    16981 taaaggcacc ggtgaatgca cgccgtatcc gcgttatgga gaaagtgatg cctcggtaat
    17041 ggcgcaaatt atgagcgtcg tgccgcaatt agagaaaggg ctgacacggg aggagttgca
    17101 aaaaatcctc cctgccggcg cagcacgtaa tgcgctggat tgtgcattgt gggatctcgc
    17161 ggcgcgaaaa cagcaggaat cgctggctga tttgatcggc ataacgcttc ccgggacagt
    17221 tatcactgca cagacggttg tcatcggtac gcctgatcag atggccaata gtgcatcaac
    17281 actctggcag gcaggcgcga aattactgaa agtgaagctg gataaccatc ttatcagtga
    17341 gcggatggtg gcaattcgca ccgctgtgcc cgatgcgacg ctgatcgttg atgcaaatga
    17401 atcctggcgt gcagaagggt tggcggcgcg ttgccagcta ttggcggatt taggcgttgc
    17461 aatgcttgaa caaccgcttc ctgcgcagga cgatgcggcg ttggagaatt ttattcatcc
    17521 gttaccgatt tgtgctgatg aaagttgtca tactcgtagc aatctgaagg cgctgaaagg
    17581 gcgctatgag atggttaaca ttaagctcga taaaacgggg ggcctgacgg aagcgctggc
    17641 gctggcgact gaagcacgtg cacaaggttt tagtctgatg ctgggctgca tgttgtgtac
    17701 ctctcgtgcc attagcgccg ctttaccgct ggtgccgcag gtcagtttcg ccgatcttga
    17761 cggaccgacc tggctggcgg tagatgtgga accggcgctt cagttcacaa cgggcgaatt
    17821 gcatctttag gatgccagcg cagcaagttt gccatcgcaa agagatattt ttcgctggct
    17881 tcgtcggagg agatgggtgg aaactctgcg gtgatgcaat gcaggttgag atccgcacac
    17941 caactgccga aagagccagg cgtttcataa ccgacgctgg ttaccagagg caattcaaac
    18001 gcctgggcca gccattcacc taattcgctg tgtctgggat cttcaataca ggccagcgga
    18061 tcgtggaatg agaccaccca ggcgggttga atgcggtgta taagctggca cagcgcctgg
    18121 gtttcaggtt cggagccagg tttgtcacct gtcagtaaaa cgacatcacg ttcttcagcg
    18181 gcgctgttcc agcgataaac ggtttcacct tccttccagt ttgccgccgg aaagtttctg
    18241 tttaaatcca caccattagc attggccctt aaccccaact ggcagccgtc aggattcaca
    18301 cacagcacca catgatgacg gcgcaaagaa ggtgtcaacg tccgcagagc acaggagagc
    18361 gtgacgatcg aagagttttc atcaccgtga gtgccagcca gaatcaaacc actttcgtga
    18421 ctggctgcag gggccggaaa ccagattaac ggcgcgccca ataatgaacg tccgtaatgt
    18481 tctgttccgg gcggaaatgc gccgcgttcg gcgcgtgggc gagttacggt catgatcgtc
    18541 tctggttcag gaagtattac aggcagtgtt gtgcaaattt tgcggattat caaatccttt
    18601 tcttcaacgc tatgttatcg atcttgtatc acttttttgc cggaagttgt ttgcattttc
    18661 attgatgtgc agatattaga attgtcgtaa tgttatatac atggcgtatt ttattcttac
    18721 tggttctaca catgttaatc gcatctgatt gatcagttca ccttaataag tttaataaat
    18781 cgtactgcac atagtacagt acgatggtag ctatataatt gatttacatg aagaatggct
    18841 tatcgccatc agtcccgggt tgattgattc ctatattctc accaatgtgt ttctcaggtt
    18901 tatcgatgat atctgtaatt aatgctgcga cactcttacg tgatacgatc gtacctttga
    18961 atggttcatt gcgtgaggta agctcataat caataatatc ttcatcagtc agccaggcag
    19021 ggcgaagaat tgtatattcc agtccagaag cctcaattgc atctgcagcc ctgcggaatg
    19081 gttttagggg ttcaccaata actgcattat tccattcgcc aaattttcct ggaacttcat
    19141 cataaatacc gagggaaaga acaaagatca gacgtttaac atcaccggct ttcatcgcag
    19201 cgatcactga atttgcctga atatctaaat cttcccccgt aaggttggca tacacaacat
    19261 cctgtccctg catggcctgc ttcagtgctg catgattcag tacatccccc ataataatct
    19321 tgctattagt gggatagggt ttgtggattt ttgctggctg acgggcaaat aaagtttgtt
    19381 taatggtttg tttatctgcc agttgattaa tcacatggcg ggctatctga ccgccagcac
    19441 caagaatcag tacatttttc atgaaacttt tcccagatta ttggcagcaa tatgccaggc
    19501 catatcacta ttacggattt gaatatgttt cattacagag tttcctcaaa gaacggagct
    19561 aatactgaaa ttgcttcagc cacatagact ttgccgtcat aaagatccat atggtttgcg
    19621 ccttcaacaa tgtgatagcg tttatcctgg cttgatgctc gatcgtacag gtcgtcactc
    19681 atccatttgc tccctgcctg gctgcccgcc acaatctgta tcggctgagt caggtacact
    19741 tccgccatat ggtaagcatc ataggtaata atctggttaa ggctgcgcaa agtagcgtaa
    19801 cccggtgctg ttggatactg cgcgcgaggg gtatggtaat actcccaggc ctgacgcagt
    19861 tcttcgttcg gcgcatcgga ctccttcatt ggtgccagtg gcatagtggc gtattctctg
    19921 ctgctaatat cgctggttcg ggcgtttgaa cccgcgtcaa cgtatggtag ggcatctata
    19981 gatttcacat tgttttccca accattacgg aacatcgaac caatattgac cgcactaaca
    20041 gtaccgatgg ccttgatgcg gcgatcctga attgcagcat tggctgtata tcctgcaccg
    20101 gcacaaattc ccatcgcacc aattcggcta ttgtcgacat aagaaagcgt tgtcaggtaa
    20161 tcaatcacgg cactgacgtc ttcagtacga atgtatgggt tttctaactg acgcggctcg
    20221 ccgccgcttt caccttgata agatgcgtca taagcaatag tgacaaatcc cttttccgcc
    20281 agtttttcgg cataggttcc agccgtttgc tctttaacgc ccccacctgg gtgagatacc
    20341 acaattgccg gatactgacg ggtttcatca aattttggag ggaaatagat cactgcagac
    20401 aaagagatgg ttggattatt gctgttagtg aagctgactt tattattcat cattccgttc
    20461 ctcatggagt tgtcggttcg ttttgacggt tggtgatatc actatagata ttgatcatta
    20521 agttgattag acccaaaatc atgattagac ctatcattta aatgattaat agattgtatg
    20581 gagatactga atgaagcgtg aagaaatcgc tgatctgatg gcgtttgtcg tcgttgcaga
    20641 ggagcgtagc ttcactcgcg cagcagcccg cctgagcatg gcgcagtcag ctttaagcca
    20701 gatagtgcgc cgtatagaag aacgattggg attgcggctt ctgacacgaa ccacgcgcag
    20761 cgttgtaccc actgaagcgg gcgagcatct tttgtctgtt cttggcccta tgttgcatga
    20821 catagattct gccctgacat ccctgagcga tctgcagaac cgcccatccg ggacaatacg
    20881 tattactact gtagaacatg cagcaaaaac gatattgtta ccagctatgc gcacattcct
    20941 gaaatcgcat cctgaaattg atatccagct taccattgat tatggtttga ccgatgtcgt
    21001 ttctgaacgt tttgatgcag gcgtccgtct gggtggggag atggataaag atatgatcgc
    21061 cattcgaatc gggccagata taccaatggc tattgttggc tcaccggatt atttttctcg
    21121 tcgaagtgct ccaacctcag tgtcacaatt aatagatcat caggcaatta atttgtatct
    21181 tcccacatcg ggtacagcaa atcgctggag attaatacgc ggtggacgtg aagttcgtgt
    21241 tcgcatggaa ggtcagcttt tactgaatac gatagacctg atcattgatg ctgcaattga
    21301 tgggcatgga ttggcgtatc taccttatga tcaggttgag cgggctatta aagaaaaaaa
    21361 actgatacgt gttttggata aattcacacc agatttaccc ggttatcacc tgtactatcc
    21421 acaccgtcga catgctggct cggcattctc attatttata gataggctga agtataaagg
    21481 tgctgtttag cactacttgc tgatacatta atttaattct tctcttaacg tattctcagt
    21541 tcctttcaac gttttggtta ttttttattc ttcgtacaat ggcgacagat gctgattatg
    21601 ataccgaaaa cgggtttgaa c
//
LOCUS       DAZGAP010000085        21540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGAP010000085 DAZGAP010000000
VERSION     DAZGAP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.085"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2934
                     /locus_tag="ACHLAM_002719"
     CDS             <1..2934
                     /locus_tag="ACHLAM_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7854224.1"
                     /translation="AAHGTDATSKITNVKDGNLTAGSTDAVNGSQLKTTNDAVAANTT
                     NIATNTTNITNLTDAVDSLGDDSLLWNATAGAFSAKHGTNGTDSKITNLLAGTVSSDS
                     TDAINGSQLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAAINTSF
                     STSLGDALLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDAINGSQLYDT
                     SKYIADTLGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWDATAND
                     GNGAFSAAHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNA
                     DGSITAPTYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKASIITNV
                     ADGNIGEGSTDAINGSQLFNTNMMIQQNSEIINQLAGNTSETYIEDNGAGINYVRTND
                     NGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIVK
                     GSRDTSVSEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQN
                     AIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAIN
                     GIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQI
                     TNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVS
                     TGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTISVGSST
                     NQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSVDYSNITLGGGNGGTTRI
                     SNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMT
                     GLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALG
                     AGIQW"
     gene            3346..5001
                     /gene="lldP"
                     /locus_tag="ACHLAM_002720"
     CDS             3346..5001
                     /gene="lldP"
                     /locus_tag="ACHLAM_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO7854225.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            5001..5777
                     /gene="lldR"
                     /locus_tag="ACHLAM_002721"
     CDS             5001..5777
                     /gene="lldR"
                     /locus_tag="ACHLAM_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO7854226.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5774..6964
                     /gene="lldD"
                     /locus_tag="ACHLAM_002722"
     CDS             5774..6964
                     /gene="lldD"
                     /locus_tag="ACHLAM_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO7854227.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            7037..7510
                     /gene="trmL"
                     /locus_tag="ACHLAM_002723"
     CDS             7037..7510
                     /gene="trmL"
                     /locus_tag="ACHLAM_002723"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO7854228.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7563..8384)
                     /gene="cysE"
                     /locus_tag="ACHLAM_002724"
     CDS             complement(7563..8384)
                     /gene="cysE"
                     /locus_tag="ACHLAM_002724"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO7854229.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8464..9483)
                     /gene="gpsA"
                     /locus_tag="ACHLAM_002725"
     CDS             complement(8464..9483)
                     /gene="gpsA"
                     /locus_tag="ACHLAM_002725"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO7854230.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9483..9950)
                     /gene="secB"
                     /locus_tag="ACHLAM_002726"
     CDS             complement(9483..9950)
                     /gene="secB"
                     /locus_tag="ACHLAM_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO7854231.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10013..10264)
                     /gene="grxC"
                     /locus_tag="ACHLAM_002727"
     CDS             complement(10013..10264)
                     /gene="grxC"
                     /locus_tag="ACHLAM_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO7854232.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10406..10837)
                     /locus_tag="ACHLAM_002728"
     CDS             complement(10406..10837)
                     /locus_tag="ACHLAM_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO7854233.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11082..12626
                     /gene="gpmM"
                     /locus_tag="ACHLAM_002729"
                     /gene_synonym="pgmI"
     CDS             11082..12626
                     /gene="gpmM"
                     /locus_tag="ACHLAM_002729"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO7854234.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12660..13919
                     /gene="envC"
                     /locus_tag="ACHLAM_002730"
     CDS             12660..13919
                     /gene="envC"
                     /locus_tag="ACHLAM_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO7854235.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13923..14882
                     /locus_tag="ACHLAM_002731"
     CDS             13923..14882
                     /locus_tag="ACHLAM_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO7854236.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14869..15903)
                     /gene="waaH"
                     /locus_tag="ACHLAM_002732"
     CDS             complement(14869..15903)
                     /gene="waaH"
                     /locus_tag="ACHLAM_002732"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO7854237.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16142..17167)
                     /gene="tdh"
                     /locus_tag="ACHLAM_002733"
     CDS             complement(16142..17167)
                     /gene="tdh"
                     /locus_tag="ACHLAM_002733"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO7854238.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARYVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17177..18373)
                     /gene="kbl"
                     /locus_tag="ACHLAM_002734"
     CDS             complement(17177..18373)
                     /gene="kbl"
                     /locus_tag="ACHLAM_002734"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO7854239.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18587..19519
                     /gene="rfaD"
                     /locus_tag="ACHLAM_002735"
     CDS             18587..19519
                     /gene="rfaD"
                     /locus_tag="ACHLAM_002735"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO7854240.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19529..20575
                     /gene="rfaF"
                     /locus_tag="ACHLAM_002736"
     CDS             19529..20575
                     /gene="rfaF"
                     /locus_tag="ACHLAM_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO7854241.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20579..>21540
                     /gene="rfaC"
                     /locus_tag="ACHLAM_002737"
     CDS             20579..>21540
                     /gene="rfaC"
                     /locus_tag="ACHLAM_002737"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO7854242.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENA"
BASE COUNT         5240 a         5719 c         5647 g         4934 t
ORIGIN      
        1 gccgcgcacg gtactgatgc caccagcaaa atcactaacg tcaaagacgg taacctgacg
       61 gctggcagca ctgacgcggt taacggctct cagctcaaaa ccactaacga tgccgtggca
      121 gccaacacca ccaatatcgc cacgaacacc accaacatca ccaacctgac tgacgctgtt
      181 gacagtctcg gtgacgattc cctgctgtgg aacgctacgg cgggtgcatt cagtgccaaa
      241 cacggcacca acggtactga cagcaaaatc accaacttac tggcaggcac tgtatcctct
      301 gatagcactg acgccattaa tggctcacaa ctttatggtt tagctgattc atttacgtca
      361 taccttggcg gtggtgctga tatcagcgat gcgggtgtat taaccgggcc aacctacact
      421 attggtggta ctgactacaa taacgtcggt gatgctctgg cagccattaa cacgtcattt
      481 agcacatcac tcggtgacgc cctactctgg gatgcgaccg ccaagggcgg tgacggtgcg
      541 tttagcgccg gtcgcggaaa agataacacg gccagcatca ttactaatgt tgcagacggt
      601 gcaatttcct ctaccagcag cgacgctatt aacggctcac agctctatga caccagtaag
      661 tacattgccg atactctggg tggtgacgca gaagtcaatg ctgacggcac aatcaccgca
      721 ccgacttatg ctatcgctgg cggcagttac agcaacgtcg gtgacgcgct ggaagcaatc
      781 gataccacgc tcgatgacgc tctgttgtgg gatgccacag ccaatgatgg taatggtgca
      841 tttagcgccg ctcacggaaa agataaaaca gccagtgtaa tcactaacgt cgctaacggt
      901 gcaatctctg ccaccagcag cgatgccatt aacggttcac aactgtatac caccaacaag
      961 tacattgccg atgccctggg tggtgacgca gaagttaacg ctgatggttc tatcactgcg
     1021 ccgacttaca ccattgcaaa tgccgagtac aacaacgtcg gtgacgccct ggatgcgctc
     1081 gacgataacg ctctgctgtg ggatgcaacg gccaatgacg gcgcaggtgc ctacaacgcc
     1141 agccatgacg gcaaggccag catcatcaca aatgttgctg atggtaacat tggcgaaggc
     1201 agtaccgacg ccatcaacgg ttctcagctg tttaacacca atatgatgat ccagcagaac
     1261 agcgaaatca tcaatcagct tgccggtaac accagcgaaa cctacatcga agataacggt
     1321 gcgggtatta actatgtacg taccaacgac aacggcttag cgttcaacga tgccagcgct
     1381 tcaggtattg gcgctacggc tgtgggttat aactctgttg ccgaaggcga tagcagcgta
     1441 gccatcggtc agggcagcta cagcgacgtt gataccggta ttgccctggg tagcagctcc
     1501 gtttccagcc gtgtaatagt taaaggttct cgtgacacca gcgtgtcgga agaaggtgtt
     1561 gtgattggtt atgacaccac ggatggcgaa ctacttggtg cgctgtctat tggtgatgac
     1621 ggtaaatatc gtcaaatcat caacgtagcc gatggttccg aagcccatga cgccgttacg
     1681 gttcgccagt tgcaaaatgc cattggtgca gtcgctacca cgccgaccaa atacttccac
     1741 gccaactcaa cggctgaaga ctcactggct gtcggggaag actcgctggc gatgggcgcg
     1801 aaaactatcg tcaatggtaa tgcgggtatt ggtatcggcc tgaatactct ggttctggca
     1861 gatgccatca acggtattgc tatcggttct aacgcacgcg ccaaccatgc aaacagtatt
     1921 gcaatgggta atggttctca gaccactcgc ggcgctcaga ctggctacac cgcctacaac
     1981 atggatgcac cgcagaactc tgtgggtgaa ttctccgtag gtagcgaaga cggtcaacgt
     2041 cagatcacca acgtcgcagc ggggtcggcg gataccgatg cggttaacgt gggtcagttg
     2101 aaagtaacgg acgcgcaggt ttcccagaat acccagagca ttactaacct gaatactcag
     2161 gtaacgaatc tggatacccg cgtcaccaat atcgaaaacg gtattggcga tatcgtaagc
     2221 accggtagca ccaagtactt caagaccaac accgatggcg tagatgctaa cgcgcagggt
     2281 aaagacagtg ttgcgattgg ctctggctct attgctgccg ctgataacag cgttgcgctg
     2341 ggtactggct ctgtggcaaa cgaagaaaat acgatctccg taggttcttc cactaaccaa
     2401 cgccgtatca ccaacgttgc cgctggtaaa aatgctactg acgcggttaa cgttgcgcag
     2461 ttgaagtctt ccgaagcagg cggcgtgcgg tatgacacca aagctgacgg ttctgttgat
     2521 tacagcaaca tcactctcgg cggtggtaat ggcggaacga ctcgcatcag caacgtttcc
     2581 gctggcgtta acaacaacga cgcggtgaac tacgcgcagt tgaagcaaag cgtgcaggaa
     2641 acgaagcaat acaccgatca gcgcatggtt gagatggata acaaactgtc caaaaccgaa
     2701 agcaagttga gcggtggtat cgcttctgca atggcgatga ccggtctgcc gcaggcttac
     2761 acgccaggtg ccagcatggc ctctattggt ggcggtactt acaacggtga atcggctgtt
     2821 gctttaggtg tgtcgatggt gagcgccaat ggtcgttggg tctacaaatt acaaggtagt
     2881 accaatagcc agggtgaata ctccgccgcg ctcggtgccg gtattcagtg gtaatcatcc
     2941 attaacgaat gggtaaacgc cacattgcct gatgcgctac gcttatcagg cctacggggt
     3001 gattcagcac taccctgtag gccggataag gcgttcacgc cgcatccggc aagataaggc
     3061 gcaccagatc aacaatctga gggcaattct ctgatgggaa ttgccctttt cttcattacc
     3121 tctctcccag aattggccct accaattctt cattgatctg acctctggtt cacaattccc
     3181 caattaaaac tcacatcacc gttgccaata cataacatct agttaactat tcattgtcat
     3241 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
     3301 caacagactc attacacggc gtgtgtggac tgcaggagac ctgcaatgaa tctctggcaa
     3361 caaaactacg atcccgccgg gaatatctgg ctttccagcc tgatagcatc gcttcccatc
     3421 ctgtttttct tttttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
     3481 acggtggcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
     3541 ctggcctcgg tgatttatgg cttcttctac ggtttgtggc ccatcgcgtg gatcattatt
     3601 gcggcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat catccgctcg
     3661 tctattcttt cgataacccc tgaccagcgc ctgcaaatgc tgatcgtcgg tttctgtttc
     3721 ggcgctttcc ttgaaggagc cgcaggcttt ggcgctccgg tagcaattac cgccgcattg
     3781 ctggtcggtc tgggctttaa accactctac gccgccggac tgtgcctgat tgttaacacc
     3841 gcgccagtgg catttggtgc gatgggtatt ccgattctgg tcgccgggca ggtaacaggc
     3901 atcgacagct ttgagattgg tcagatggtg gggcgtcagt tgccgtttat gaccattatc
     3961 gtgctgttct ggatcatggc gattatggac ggttggcgcg gaatcaaaga aacgtggcct
     4021 gcggtcgtgg tggcaggtgg ttcgtttgcc attgctcaat acctcagctc taacttcatt
     4081 gggccggaac tgccagacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
     4141 ctcaaacgct ggcaaccagt gcgcgtattc cgtttcggtg acttgggggc gtcacaggtt
     4201 gatatgacgc tggctcacac cggttacacc gcaggtcagg tgcttcgcgc ctggacaccg
     4261 ttcctgttcc tgaccgccac cgtaacgctg tggagtatcc cgccgtttaa agccctgttc
     4321 gcttcaggcg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
     4381 ctggttgccc gtatgccgcc agtggtcagc gaagcgacag cgtatgccgc cgtgtttaag
     4441 tttgactggt tctctgccac cggtaccgcc attctttttg ctgccctgct ctcaattgtc
     4501 tggctgaaga tgaaaccatc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
     4561 gctctgccta tctactccat cggtatggtg ctggcgttcg cctttatctc gaactattcc
     4621 ggactatcat caacgctggc gctggcactg gcgcacaccg gccatgcatt caccttcttc
     4681 tcgccgttcc tcggctggct tggtgtcttc ctgaccggat cggatacctc atctaacgcc
     4741 ctgttcgccg ccctgcaagc cactgcagca caacaaattg gcgtttctga cctgttgttg
     4801 gttgccgcca acaccaccgg tggtgtcacc ggtaagatga tctctccgca atctatcgct
     4861 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
     4921 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
     4981 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
     5041 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
     5101 aacgtcaact ggcgatgcaa ctcggcgtgt cgagaaattc actgcgcgag gcgctggcga
     5161 aactggtcag tgaaggtgtg ctgctcagtc gacgtggcgg cgggacgttt attcgctggc
     5221 gtcatgatac atggtcggag caaaacatcg tccagccgct gaaaacgctg atggccgatg
     5281 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcgt
     5341 ggcatgcggc aatgcgcgcc acaccaggcg aaaaagagaa aattcagctt tgctttgaag
     5401 caacgctaag tgaagacccg gatctcgcct cacaagcgga cgttcgtttt catctggcga
     5461 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
     5521 tgcaatcctc tgtgaagcat agccgccagc ggatgtatct ggtacccccg gttttttcac
     5581 agctgaccga acaacatcag gcagtcattg acgccatttt tgccggtgat gctgacgggg
     5641 cgcgtaaagc aatgatggcg caccttagct ttgttcacac caccatgaaa cgattcgatg
     5701 aagatcaagc tcgccatgca cgcattaccc gcctgcctgg tgaccataat gagcattcga
     5761 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cacaacgcat
     5821 tctgccgccg ttcctgttcc actatatgga tgggggtgca tattctgaat acacgctgcg
     5881 ccgcaacgtg gaagatttgt cagaagtggc gctgcgccag cgtattctga aaaacatgtc
     5941 tgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tggcgctagg
     6001 tccggtaggt ttgtgtggca tgtatgcgcg acgcggcgaa gttcaggctg ccaaagccgc
     6061 ggatgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
     6121 ggctccagcc attaaacgtc cgatgtggtt ccagctttat gtgctgcgcg atcgcggctt
     6181 tatgcgtaac gcactggagc gagcaaaagc ggcaggctgc tcgacactgg ttttcaccgt
     6241 ggatatgccg acaccgggcg cgcgttaccg tgatgcacac tcgggtatga gcggcccaaa
     6301 tgcggcaatg cgccgttact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
     6361 cctgaacggt cgtccacatg atttaggtaa tatctcggct tacctcggca aaccgaccgg
     6421 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
     6481 ccttgagtgg atccgcgaat tctgggatgg tccgatggtg atcaaaggga tcctcgatcc
     6541 ggaagatgcg cgcgatgcag tacgttttgg tgctgatggg attgtggttt ctaaccacgg
     6601 tggccgccag ctggacggtg tactctcttc cgctcgtgca ttgcccgcta ttgcggatgc
     6661 ggtgaaaggt gatattgcca ttctggcaga tagcggaata cgtaacgggc ttgatgtcgt
     6721 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
     6781 ggcaacagcg ggccaggcgg gcgtggcaaa tctgctgaat ctgatcgaaa aagagatgaa
     6841 agtggcaatg acgctgactg gcgcgaaatc gattagcgaa attacgcaag attcgctggt
     6901 gcaggggctg ggtaaagagt tgccggcggc actggctccg atggcgaaag ggaatgcggc
     6961 atagttgggg gtgaggggaa aaggccgtac caatcaccaa ttttctgcta tcctgccccc
     7021 gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc cgccaaatac
     7081 tggcaacatc atccgtcttt gcgctaatac cggctttcgt ctgcatatca tcgaaccaat
     7141 gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg ctggactatc acgagtttac
     7201 cgccgttacg cgtcatcatg actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct
     7261 gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaagctatc aggatggcga
     7321 ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg atgccctgcc
     7381 cactgaacaa aaaattcgta ttccaatggt gccggacagc cgcagcatga acctgtctaa
     7441 tgcggtgtcg gtcgtggtgt atgaagcctg gcggcagttg gggtatccgg gagcggtgtt
     7501 gagagattag ttactgtagg ccggatagat gattacatcg catccggcac gatcacagga
     7561 catcagatcc catcaccata ctcaaacgta tggttaatgc cgttgaaatg ctgatccata
     7621 tccattgacg gtttatcgct atccggcttg ccgacaatgc gtgccggaac gccagcggca
     7681 gtggtgtgcg gcggcaccgg ttgcagcacc acggaacctg cgccaatctt cgcgccgcgc
     7741 ccaacttcaa tattaccgag aattttcgcg cccgcgccaa tcatcacgcc ttcacgaatt
     7801 ttcgggtgac ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc
     7861 gatacgtcgt tttcaatcac cgccgtttca cccacaacga tgcccgtcgc gtggtcgagc
     7921 atgataccgc gaccaatttt tgctgccggg tgaatatcaa cctggaacgt tacagagacc
     7981 tggttttgta gaaaaatagc cagcgcgcga cggccctgat tccacaacca gtgaccgatg
     8041 cgatacgcct gcaaagcatg aaaacccttc aggtatagca acggggttga gtatttatcc
     8101 accgccgggt cacgggtacg caccgcctga atatcacagg ccgcagaggc gatcatttcc
     8161 gggtcggcgg cgtaggcttc ttccaccact tcacggatag caatagccgg cataatgggc
     8221 gatgacagct tgttcgccag catgtagctc agtgcactgc caaggttttc gtgcttgagt
     8281 agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt ctgccagcgt tctggcttca
     8341 gctttaatat tgttccagac aatttccagt tcttcacacg acattgctta ctccacacga
     8401 taagataatg accggcccgt tctgcgcggg ccgggtcata gtggtaacaa aggttccctg
     8461 ggtttagtgg ctgctgcgct cgtccttgcg tgcacgacct agtaaagtca atgctgcctc
     8521 gcgcgcgttt tttccgcaat ataatacttg ataaatttcc tcggttattg gcatttcaac
     8581 gccgaagcga tgcgccagtt cgcggacttc tttcgtattg cggtagcctt ccaccacttg
     8641 accaattttc tcctgcgcgc tttgtacatc catgccctga ccgagcatca tgccaaaacg
     8701 gcggttacgc gactggttgt cggtacaggt cagcaccaga tcgcccagcc ccgccatgcc
     8761 cataaaggtg gcaggatcgg cacccagcgc cgcgccaaga cgcgacatct cagccagccc
     8821 acgggtgatc agcgccgtac gcgcattcgc gccaaaaccg ataccgtcgg acatccccgc
     8881 gccaatggca atgacgtttt tcaccgcgcc gccaagctgc acaccgatga aatccgggtt
     8941 gctgtaaacg cggaaacttt tgccacagtg cagcaattgc tggaggtcat cggcaaaggt
     9001 ctggtccgtc gaggccagcg aaatggctgt cggtaaacct gccgccagtt ctttcgcaaa
     9061 cgttgggcca gagataaccg ccagcggaat ttgatcgcct aaggcctcac gcgctacgtc
     9121 ctgtaacaga cgtccggttt ctgcttccag ccctttggtc gcccacacca gacgcgcatc
     9181 aggacgcatc agcggtttaa tctggcgcag cacttcacca aagacatggc tgggtacgac
     9241 gacgagaata ttacggctgg ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt
     9301 atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc
     9361 gatatgttca gggtcatggc cccagaggac aacctcgtgg ccatttcttg ccagggtaat
     9421 ggcaagagcg gtgccgtacg agccggcacc gatcacagtc attgaagcat tacgttggtt
     9481 catcaggcat cctgatgttc ttcagtacct tcgccagcct gctgctgcaa atagttcatg
     9541 aacagcgcat cgaagttaac cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc
     9601 atgctggtga tgcactcacg agcatacggg aacagaatgt tcgggcagta tgcacccagg
     9661 caatgtgcca tctgggtgcc ttcgataccc gcgatggaga aaataccgcc ctgctgaact
     9721 tcacacagga acgcggtttc ttcgcccagg gaggccgtta cggtaacacg cagtaccact
     9781 tcgtaaacgt catctgccag ttgagaagat gccgtatcca gatcaagttt aacttctggc
     9841 tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt cgaaagaaat atccttggta
     9901 taaatacgtt ggatctggaa agtcatttcg gtgttgtttt gttctgacat gtgtagaaaa
     9961 cccttaagtg ttgtccttaa atactgcgca atgtaatgcc aacgttcaca cgttatttca
    10021 gcaggggatc cagtccacca cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt
    10081 gctgtgcgtc aataaaaatc tggggaaccg tggtgcgacc gctgcgtttg atcatctctt
    10141 cacgcttagc ggcgttgcca tcgatcggca actcctgaaa actcacgccc ttgctgctca
    10201 gcagtgcttt cgcacgatgg caatacgggc aggtttcttt ggtatagatt tcaacattgg
    10261 ccataactta gctcctgttt ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat
    10321 ggagtgtccc cagaagctta taacaataag tcgctggggg cctaattgca gctaacggcc
    10381 tgcatcatga aagacgacag gtaaattatt tgccgcgcac cagaggtaag ttttcgccag
    10441 cccagccagc aacgccctct ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg
    10501 cgtttgcagg ctcctggcac tgcatgccag aaccgtctac cacgataacc ggtttgtctt
    10561 tgtgcttctc aagctcacca acattgttgg ctttgatttc gctcggcaac aggttgatag
    10621 aacctgcgat atggccttta cggaagtcat cacgctgacg taaatccaca accacagcat
    10681 cttctttgtt gatcagacgc gtagcttcac cacgagtaat caccttcact ttcgaggtca
    10741 ggctcttaaa cgtagtcaca ataaccgcca ccagtaacgc gatccaggcg atactcagta
    10801 tgggatgacg gccaacaaat tgcataattt cttgcatggg gggtaacaac tcccgacgta
    10861 gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg cagcaaatac agccagcgcg
    10921 ttaactggaa tgcaattttg cggggcgcga cgaaaaaaaa gcgcaaatct ggcatcggct
    10981 tgctgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta
    11041 aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac
    11101 ctatggtact ggtgattctg gatggctatg gctatcgtga agaacagcag gataacgcca
    11161 tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga
    11221 tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag
    11281 gtcacgttaa cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt ctggacgttg
    11341 aaatcaaaga tcgcgctttc ttcgctaacc cggtactgac tggcgcggta gataaagcga
    11401 aaaacgcagg caaagcagtt cacattatgg gtctgctctc cgcaggtggt gtacacagcc
    11461 acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct
    11521 acctgcacgc attccttgac ggtcgcgaca ctccgccgcg cagtgctgaa tcctcgctga
    11581 aaaaattcga agagaaattt gccgcactgg gcaaaggccg cgtagcgtcc atcattggtc
    11641 gttactacgc catggaccgc gataaccgtt gggatcgcgt tgaaaaagct tatgacctgc
    11701 tgactctggc gcagggcgag ttccaggccg ataccgccgt tgctggtttg caggctgctt
    11761 atgctcgcga cgaaaacgac gaattcgtga aagcgaccgt tattcgtgca gaaggtcagc
    11821 cagacgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgtg
    11881 cacgtgaaat cactcgtgca ttcgtaaatg ccgattttga cggcttcgcg cgtaagaaag
    11941 tggttaacgt cgatttcgtg atgctgaccg aatacgctgc cgacatcaaa accgcagtgg
    12001 cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa
    12061 ctcagctgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg
    12121 gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta
    12181 cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcaa
    12241 tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc
    12301 acaccggagt gatggaagcg gcggttaaag cggttgaagc gctggatcac tgcgtggaag
    12361 aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta
    12421 acgctgagca gatgcgcgat ccggcaacgg gtcaggcaca cacggcacat accaacctgc
    12481 cagttccgct gatttacgtt ggcgataaga acgtgaaagc ggttgaaggc ggcaaacttt
    12541 ctgatatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg
    12601 gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca
    12661 tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc
    12721 ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca
    12781 aatctattca ggccgatatc gccgcgaaag agcgcgcggt acgccaaaag caacaacaac
    12841 gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctcggaa gccacccgta
    12901 agctgcgtga aacgcaaaac acgctcaatc aactgaataa acagattgat gagatgaacg
    12961 cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac
    13021 tagatgccgc attccgtcag ggcgagcata ccggtattca gctgattctc agcggtgaag
    13081 aaagccagcg tggacagcgt ttacaggctt atttcggcta tctcaaccag gcgcgacaag
    13141 aaaccattgc tcagttgaaa caaacgcgtg aagaagtcgc tatgcaacgc gccgagctgg
    13201 aagagaaaca gagcgagcaa caaacgctgt tgtatgagca gcgcgcccaa caggcgaagc
    13261 tgacccaggc gctgaacgag cgtaaaaaga cgctggcagg gctggagtct tccatccagc
    13321 aaggtcagca acagttgagc gagctgcgcg ccaacgaatc ccgtctgcgt aacagcattg
    13381 cccgtgcgga agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg
    13441 ttcgcgaccg ccagaaagaa gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg
    13501 aaaaatcgct gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag gcattctggc
    13561 cagttcgcgg gccgacgctg catcgctatg gcgagcagct acagggtgaa ttacgctgga
    13621 aaggaatggt tatcggtgcc tctgaaggta ctgaagttaa agccattgcc gacggtcggg
    13681 tgattctggc tgactggctg caaggctacg gtctggtggt ggtggttgag catggtaaag
    13741 gcgacatgag tctttacggc tataatcaga gcgcactggt gagcgttggt tcgcaggttc
    13801 gcgcgggcca gccgattgca ctggtgggca gcagtggcgg tcagggtcgg ccttcactct
    13861 atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg ggaagataag
    13921 ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc
    13981 ccgtaattgc tggcaagctt gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg
    14041 aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc gattcaccgc
    14101 acgccagaga gatggcaacc aaagcgcata acagcggtca cgaagtgttg attcatctac
    14161 cgatggcacc actgagtaaa cagccgctgg agaaaaatac gctacgcccg gagatgagca
    14221 gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat gccgtgggga
    14281 tcaacaacca catgggcagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc
    14341 aggcgctgga gcgttacaat ctttacttcc tcgacagcgt aaccatcggt aatacccagg
    14401 cgatgcgcgc agcgcaaggc actggcgtga aggtgatcaa acggaaagtg ttcctcgacg
    14461 attcgcaaaa tgaagcggac atccgtgtgc agttcaatcg cgcaattgat ctggcgcgtc
    14521 gcaacggttc aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgctacaac
    14581 agatggttta taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg
    14641 aaccgcaggt tgatacgtct acaccgccga aaaacactgt gcccgacaca ccgcgtaatc
    14701 cgttccgtgg cgtgaagctc tgcaaaccga agaaaccgat agaacctgtt tatgctaatc
    14761 gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccaac
    14821 atcagtggca aggctggggc aaacagcctg aagcggcaaa acttaacgct agcgtaaatt
    14881 aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg
    14941 gaaagaccac agtaacgcct gataacccac cttcacgctg cgcacattgg taatcaggcg
    15001 cttatacata ccggaagtaa atatctcggc aatcatccgt tggcgggtaa taatatccgg
    15061 ctctttgcgc accgcatggc aaacacgcaa tgcttcgtaa gttatttgct gatgaaattc
    15121 cggataaatc gtaattttgt cggcataatt tcgatttaat ttctctagca ggcgggtaat
    15181 cttaatatag tgacgttgat aattaaggtt tttattccct tgtctattca accgactcac
    15241 tgacgtatta tgcagataat atttatataa cgattgctcg gtatatcgcg cacgcagcgc
    15301 attaaacata aattctgttg tccagacaat atcctgatga tgtaatccgg caataaattt
    15361 aatgttattt ttaacaataa catcgcggcg ataaaccccc atccagacaa cgtgagtcca
    15421 acggcgggaa gaaagcccca tccgcaacca atccgggccg gttaatacgc cggttgagcg
    15481 aaggcgatcg gtggggatgg attgccaggt ttctcccgtt tcacgaaaac accagtcagc
    15541 gttgcactgc gccacgtcga ggtcgtcctc taacgccatg gtcatcagcg tttcgtacat
    15601 ggtgggatag acctcatcgt cagcatcgac aaaagcgaca tatttgcccg tcgccacctc
    15661 gatcccacga ttacgcgcta ccgatgcgcc agcattcgcc tgatgcaaca aacgaacgtg
    15721 cggatagttt tctgcgtaat gctttgctat ttcaacagaa ttatccgttg aaccatcgtt
    15781 aataataatg atttccagag cagtccaggt ttgcgtaatt aaagattcca tacacgtgcg
    15841 gaaatcatcg cccgcattat ataacgggat aataacacta agtttattgg tgctgttcat
    15901 cataaattac cgacgacctc tgaagttatc ctgacgtttt acaggctttg tattaagaaa
    15961 ctattaagcc tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat
    16021 aatgagaaca gctatataag agatcagcgc aattacgaac tctttattac actgacagga
    16081 ttttacatat ttaacggatt atcctcagga aaaagggctg gaataccagc ccttgtttgt
    16141 gttaatccca gctcaggata actttcccgg actggcccga acgcatggcg tcaaagccct
    16201 tctggaaatc atcgatagag aaacgatgag taatgatcgg cgaaagatcg aggccagact
    16261 gaatcagcgc cgccatcttg taccaggttt caaacatctc acgaccgtaa atacctttaa
    16321 tgaacaagcc tttaaagatc actttggtcc agtctatcga catgtcagaa ggtggaatac
    16381 ccagcatcgc aatacgaccg ccgtggttca tggtgtcaag catggtacga aacgctggcg
    16441 gcgcaccgga catttccaga ccgacatcaa agccttcggt catgcccagt tctgccatca
    16501 catcattgag gttctcttta gcgacgttaa ccgcacgggt gatacccatt ttacgcgcca
    16561 actcaaggcg gtattcgtta acatcagtaa tcaccacgta acgtgcacca acgtgtttcg
    16621 ccaccgccgc cgccataata ccaatcgggc ctgcaccaga gaccagcaca tcctcgccca
    16681 ccagatcgaa cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca
    16741 gatcatcgga gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatact
    16801 cggcgaagca gcccggacgg ttaacgccga cgccgatcgt gttgcggcac agatgggtac
    16861 gaccgccacg acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat
    16921 cgccaatctt gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat
    16981 gacccacgac catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt
    17041 gaacgtcagt cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga
    17101 gttccggtac aggaacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg
    17161 ctttcatctc acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt
    17221 ctactgcacg cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg
    17281 cctgaccttt cggaacgacc ggatagaaga aaccggtgac gtaaatcccc tctttttgca
    17341 gctcacgggc aaatttctgc gccactaccg catcgccaag catgaccgga ataatggcgt
    17401 gatcggctcc cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt
    17461 tcgcccacag acggtcacgc agctcgctac ccgcttcaac catctccagc actttgatgg
    17521 aagcggcaac aatggccggt gccagcgagt tggagaacag gtacggacga gaacgctggc
    17581 gcagccactc aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg
    17641 ctttaccaag cgtaccggtg ataatatcga cccgccccat cacatcgcag tattcatggg
    17701 aaccacgacc gttttcaccg acaaaaccta ccgcgtgtga gtcatctacc atcaccaggg
    17761 catcatattt atctgccaga tcgcaaacgc ctttcaggtt ggcaatcacg ccgtccatcg
    17821 agaacacacc atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca
    17881 gacgtgcttc cagctcctgc atatcgttgt tggcataacg atagcgttta gctttgcaca
    17941 gacgcacacc atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg
    18001 cgcccaacag cgtttcgaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg
    18061 catcttccat ccccaggaag gccgccagtt tttgttcaag ctctttatgg ctgtcctgag
    18121 tgccgcaaat aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg
    18181 ccgccgcaat cagatccgga tgattcgcca gtccgagata gttattggca caaaagttaa
    18241 tgacgtggct tccatcagcc acagtgatat ctgcttgctg cgcagacgta ataatgcgct
    18301 cttctttaaa caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa
    18361 attctccacg cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt
    18421 ataggttgcg acgagatgac gcgttaacac ttctgcaaaa ttcaggataa ataacgtcca
    18481 gccccctcac ttttggtgaa tggctgaagc caacgtcatg tagtgatatg atagaagtat
    18541 tcgtgtctga gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac
    18601 cggcggcgcg ggctttatcg gaagcaacat cgttaaagcc ctgaatgata aaggcatcac
    18661 cgatattctg gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct
    18721 ggatatcgcg gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga
    18781 gttcggcgat gtcgaagcga ttttccacga aggcgcgtgc tcttccacca ccgagtggga
    18841 cggcaagtat atgatggata acaactatca atactccaaa gagctgctgc actactgtct
    18901 ggagcgcgaa atcccgttcc tgtatgcctc ttccgcagcc acttacggtg gacgcacctc
    18961 cgactttatt gaatcccgcg aatatgaaaa accgttgaat gtctacggtt actcaaaatt
    19021 cctgtttgat gaatatgttc gccaaatcct gcctgaagcg aactcacaga ttgttggctt
    19081 ccgttatttc aacgtttatg gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc
    19141 tttccatctc aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga
    19201 gaacttcaaa cgtgacttcg tctatgtggg cgatgtggca gatgtaaacc tgtggttcct
    19261 ggaaaatggc gtttccggca tcttcaacct cggtactggt cgtgcggaat ccttccaggc
    19321 agtagcagat gctacgcttg cttatcacaa gaaaggccaa atcgaataca ttccgttccc
    19381 ggataaactg aaaggccgct accaggcgtt cacgcaggca gatctgacaa atctgcgcgc
    19441 ggcgggttac gacaaaccgt ttaaaaccgt tgccgaaggc gtaacggaat acatggcctg
    19501 gctgaatcgt gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt
    19561 tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc
    19621 gataatcgac gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt
    19681 taacgaagct attcctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa
    19741 actgggtcat agcctgcgcg aaaaacgtta cgaccgcgcc tacgttttac ccaactcctt
    19801 caaatccgca ttagtgcctt tcttcgcggg tattcctcat cgcaccggct ggcgcggcga
    19861 aatgcgctac ggtttactca acgatgtgcg tgtgctggat aaagaagcct ggccgctgat
    19921 ggtggaacgc tatgtggcgc tggcctatga caaaggtatt atgcgtaccg cacaagatct
    19981 gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg
    20041 taatcaattt tcgctttcat cagaacgtcc gatgattggt ttttgcccgg gtgcggagtt
    20101 tggtccggca aaacgctggc cacactacca ctatgcggag ctggcaaagc aactgattga
    20161 tgaaggttat caggtggttc tgtttggctc ggcgaaagat catgaagcgg gcaatgagat
    20221 tcttgccgct ttgaataccg agcagcaggc atggtgtcgt aacctggcgg gggaaacaca
    20281 gcttgatcaa gcggttatcc tgattgcagc ctgtaaagcc attgttacta acgattctgg
    20341 cctgatgcac gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccgagtag
    20401 cccggacttc acaccgccgc tatcccataa agcgcgcgtg atccgcctga ttaccggcta
    20461 tcacaaagtg cgtaaaggtg acgctgcgga gggttatcac cagagcttga tcgacattac
    20521 tccccagcgc gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat
    20581 gcgggttttg atcgttaaaa catcgtcgat gggcgatgtt ctccatacgt tgcccgcact
    20641 cactgatgcc cagcaggcaa tcccagggat taagtttgac tgggtggtgg aagaagggtt
    20701 cgcacagatt ccttcctggc acgccgccgt tgagcgagtt attcctgtgg caatacgtcg
    20761 ctggcgtaaa gcctggttct cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc
    20821 gctacaagca gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcggc
    20881 gctggtgacg cgtctggcgc atggcgtaaa gcatggcatg gactggcaaa ccgctcgcga
    20941 acctttagcc agcctgtttt acaatcgtaa gcatcatatt gcaaaacagc agcacgccgt
    21001 agaacgcacc cgcgaactgt ttgccaaaag tctgggctac agcaaaccgc aaacccaggg
    21061 cgattatgct atcgcacagc atttcctgac gaacctgcct acagatgctg gcgaatatgc
    21121 cgtatttctt catgcgacga cccgtgatga taaacactgg ccggaagaac actggcgaga
    21181 attgattggt ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca
    21241 tgaggaagaa cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa
    21301 gatgagtctg gaaggcgttg cccgcgtgct ggccggggct aaatttgtag tgtcggtgga
    21361 tacggggtta agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc
    21421 taccgatccg ggattaattg gtgggtatgg gaagaatcag atggtttgta gggctccggg
    21481 gaatgagttg tctcaattga cagcaaatgc tgttaagcaa ttcattgaag aaaacgctga
//
LOCUS       DAZGAP010000086        21439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGAP010000086 DAZGAP010000000
VERSION     DAZGAP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.086"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..324
                     /gene="hokA"
                     /locus_tag="ACHLAM_002738"
     CDS             172..324
                     /gene="hokA"
                     /locus_tag="ACHLAM_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO7854243.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(512..724)
                     /gene="cspA"
                     /locus_tag="ACHLAM_002739"
     CDS             complement(512..724)
                     /gene="cspA"
                     /locus_tag="ACHLAM_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO7854244.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1005..1295)
                     /locus_tag="ACHLAM_002740"
     CDS             complement(1005..1295)
                     /locus_tag="ACHLAM_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO7854245.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1729..2439
                     /locus_tag="ACHLAM_002741"
     CDS             1729..2439
                     /locus_tag="ACHLAM_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO7854246.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2489..3463)
                     /gene="ghrB"
                     /locus_tag="ACHLAM_002742"
     CDS             complement(2489..3463)
                     /gene="ghrB"
                     /locus_tag="ACHLAM_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO7854247.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3567..4226)
                     /locus_tag="ACHLAM_002743"
     CDS             complement(3567..4226)
                     /locus_tag="ACHLAM_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO7854248.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4379..6712
                     /gene="bisC"
                     /locus_tag="ACHLAM_002744"
     CDS             4379..6712
                     /gene="bisC"
                     /locus_tag="ACHLAM_002744"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO7854249.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVMVLWSANPLNTLKIAWNASDEQELSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6681..7121)
                     /locus_tag="ACHLAM_002745"
     CDS             complement(6681..7121)
                     /locus_tag="ACHLAM_002745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7854250.1"
                     /translation="MIREVQHSELPAILELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7118..7681)
                     /gene="tag"
                     /locus_tag="ACHLAM_002746"
     CDS             complement(7118..7681)
                     /gene="tag"
                     /locus_tag="ACHLAM_002746"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO7854251.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7839..8537
                     /locus_tag="ACHLAM_002747"
     CDS             7839..8537
                     /locus_tag="ACHLAM_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO7854252.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8766..9974
                     /locus_tag="ACHLAM_002748"
     CDS             8766..9974
                     /locus_tag="ACHLAM_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854253.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10298..11989
                     /gene="eptB"
                     /locus_tag="ACHLAM_002749"
     CDS             10298..11989
                     /gene="eptB"
                     /locus_tag="ACHLAM_002749"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO7854254.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            12081..12157
                     /locus_tag="ACHLAM_002750"
     tRNA            12081..12157
                     /locus_tag="ACHLAM_002750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12115..12117,aa:Pro,seq:cgg)
     gene            12902..14509
                     /gene="dppA"
                     /locus_tag="ACHLAM_002751"
     CDS             12902..14509
                     /gene="dppA"
                     /locus_tag="ACHLAM_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO7854255.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(14572..14799)
                     /locus_tag="ACHLAM_002752"
     CDS             complement(14572..14799)
                     /locus_tag="ACHLAM_002752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854256.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            14817..15836
                     /gene="dppB"
                     /locus_tag="ACHLAM_002753"
     CDS             14817..15836
                     /gene="dppB"
                     /locus_tag="ACHLAM_002753"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO7854257.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15846..16748
                     /gene="dppC"
                     /locus_tag="ACHLAM_002754"
     CDS             15846..16748
                     /gene="dppC"
                     /locus_tag="ACHLAM_002754"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO7854258.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16759..17742
                     /gene="dppD"
                     /locus_tag="ACHLAM_002755"
     CDS             16759..17742
                     /gene="dppD"
                     /locus_tag="ACHLAM_002755"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO7854259.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17739..18743
                     /gene="dppF"
                     /locus_tag="ACHLAM_002756"
     CDS             17739..18743
                     /gene="dppF"
                     /locus_tag="ACHLAM_002756"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO7854260.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18773..20044)
                     /locus_tag="ACHLAM_002757"
     CDS             complement(18773..20044)
                     /locus_tag="ACHLAM_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO7854261.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20520..20627
                     /locus_tag="ACHLAM_002758"
     CDS             20520..20627
                     /locus_tag="ACHLAM_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7854262.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
     gene            21003..21110
                     /locus_tag="ACHLAM_002759"
     CDS             21003..21110
                     /locus_tag="ACHLAM_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7854263.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(21115..>21439)
                     /locus_tag="ACHLAM_002760"
     CDS             complement(21115..>21439)
                     /locus_tag="ACHLAM_002760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854264.1"
                     /translation="GLDARMAPVVLSGAYLQRAGVFSLQQPMPPGIKPPQHCASPEPF
                     RLAADSTTVFLFRNPSLLHSVLRIPQNIIRLQPLHFSYEKGRRVLSLRPGPGANCHYG
                     SLTPV"
BASE COUNT         5138 a         5277 c         5687 g         5337 t
ORIGIN      
        1 cttattaagc tagaatagct gtgggtgctt gagactgttt gtctcaggta ttcaccgaaa
       61 ggcagacaga gaaaagcccc acctgactat aaatcaaagt gaggctaccc tatgcctgaa
      121 caccataagg ttagcctctt actcgttgga aatcaacaca ggaggctggg aatgccgcag
      181 aaatatagat tgctttcttt aatagtgatt tgtttcacgc ttttattttt tacctggatg
      241 gtaagagatt cactgtgtga attgcatatt aaacagggaa gttatgagct ggcggcattt
      301 ttagcctgca atttgaaaga gtaagagact ttggcgggaa gttattcccg cctttcttac
      361 ggcgttgcgc attctcattg cacccaaatt tattcttcac aaaaataaca ctagattcta
      421 ttacgcgatc gattatttat ttcctgaaaa acaaataaaa aaatccccgc caaatggcag
      481 ggatcttaga ttctgtgctt ttaagcagag attacaggct ggttacgtta ccagctgccg
      541 ggcctttagc gccgctttcg atggtgaagg acactttctg accttcgtcc agagatttgt
      601 aaccatcgtt ctggatagca gagaagtgta cgaacacgtc tttagagcca tcgtcaggag
      661 tgatgaagcc gaagcctttg tcagcgttga accattttac gataccagtc attttaccgg
      721 acatagtgta ttacctttaa taattaagtg tgcctttcgg cgatatggcg tgctttacag
      781 attttgaagc gttaaaggaa tgtgcactac gaggggtatc aacgataact cttgaaggga
      841 cttgccttac tacactgctt taatggtctg tacgtcaaac cgttgatgtg cattaagcca
      901 cgcattggcg ggtgatgcaa caattatttt tcatatttat gattaatcgg ttggaaaacg
      961 gtcctgtcat caggaccgta aacagcaata aagtggataa aagtctattc catcaactgc
     1021 ttacttaatg ccgggttggc ttgaatcaaa cgcatcaatt ttagttcggc acttgaaggc
     1081 ttcacgcgtc tggattccca ttcctttacc atggcgactg atacgcccaa aacccgggcg
     1141 aaatcatcga tttttaaccc tgtccctttt cgtaactgct caatttcggt acaggacgtt
     1201 gttctgtgcg tcagggtaat tttctgcgtt tcatctttaa aaacaatctg ttccaggctg
     1261 ctcaacagct catgcattgg atctttatat tccattgaaa actcctcaaa tcacactgcg
     1321 ggatcgtgaa ttgcatcgaa gctcattaag aatagtcggg aaaaacagac atagcggggt
     1381 gtagtcaggg catgctctgg ctatgattcg attcagccag tcatttagcg tgacttatag
     1441 ccacctaacg atctgataaa tgccgatata tctcttacca ctcccggttt gtaaagattt
     1501 cgaaaatcta acgttctgcg aaggcgtaaa tctcaggcta caaaaaaagc aacggcacta
     1561 tcatttttta acaatgaaac aacaaattga ccttgtttca ttttctccat gattttacca
     1621 ggaagattac gtagaaaggg tatcctgcaa gcctgtcctg gactatcctt gtcacgtcag
     1681 acacgcgtgt gtcgttgtgc gctttttttg ggtgaaagga gtaagaaaat ggcgacagga
     1741 aagtcctgct ctcgctggtt tgcgcctctt gcggcgttat taatggtagt tagcctgagt
     1801 gggtgttttg ataaagaagg cgatcagcgt aaagcgttta tcgacttcct gcagaataca
     1861 gtgatgcgta gcggtgaacg tctaccaacc ctgactgccg atcagaaaaa acagtttggt
     1921 ccttttgtct ctgattacgc gattctgtat ggttattctc agcaggtgaa tcaggcgatg
     1981 gattccggtc tgcgcccggt tgtagacagc gttaacgcaa ttcgcgtacc gcaggattac
     2041 gttacgcaaa gcggcccact gcgtgaaatg aacggttctc tgggcgtact ggcgcaacag
     2101 ctgcaaaatg cgaagttgca ggccgatgct gcgcactctg cgctaaaaca gagcgatgac
     2161 ctgaaaccgg tcttcgatca ggcattcacc aaagtggtaa cgacgccagc tgatgcgttg
     2221 caaccgttga ttccggcggc acaaactttc acgcaacaac tggtgatggt tggagactac
     2281 attgctcagc aaggtactca ggtgagcttt gtcgcaaatg gcatccagtt cccgacttcg
     2341 cagcaggcaa gtgaatataa caaactgatt gcgccattac cggcacaaca tcaggcgttt
     2401 aatcaggcct ggactacagc agttactgca acccaataaa gagtaaaagc ccgagcgatt
     2461 atcgggcttt actccacgca gtcgcggctt agtccgcgac gtgcggattc acacagttct
     2521 tctcaacctt tccttgtaac gcatcaatca agttatccac ggcacaggcg gccatgccat
     2581 aacgcgtctc atgagtggca gatccaatat gcggtactgc gacgacgttg gccattgaga
     2641 gcaacggcga atctactgac agtggctctt gttcgaagac atccagcccg gcagcgtgaa
     2701 tttccccttt ctgcaatgca gcaatcagtg cattttcatc aaccaccggg ccacgtccgg
     2761 cattaatgaa aatggcggag gatttcattt tggcgaattg ttctgcgcca aacagatgat
     2821 gcgtttcatc agttaacggc aggatcaggc aaacgaaatc tgactcttgt aacagtgtat
     2881 ccaaatcgca gtagcgggcg ttgaagcgtt cttctgcttc tttatggtgg cggcgcgcgt
     2941 tatagaggat gggcatgttg aagccaaagt gcgcacgttg tgccagcgcc atgccgatcc
     3001 gtcccatccc gacaatgccc agtgttttat ggtgaacgtc agtgccgtac cagtccgggc
     3061 ctatgctcgc ggtccattcg cctgctttta ctcgttctgc tacctccaca acccgacgag
     3121 cggtagacaa taccagcgcc atcagcgtat cggcgacggt ttctgttaat acggttggcg
     3181 tgtgcatcag cagaattttt cgggcggtaa gcgcatcgac atcaaaattg tcatagccta
     3241 cggagatcgt tgatgtggca cgcagtttcg gcattttttc cagcaatgcg gcatcaacat
     3301 tctcgtttga acccagtaaa ccttcagctt cggcaagaat tgctgcattt tgttcgatgg
     3361 tttgtgggct gaggtttgcc acctggtgaa cggtgaaatg ctcttgcagg cgttgcagta
     3421 aatcatcagg taaggctttg tagaggataa cggacggctt cattcttctc tccattcact
     3481 gataaggcga tcaccttagc ctgaaatatg atgaaggaaa actgaagaat gccaggtgat
     3541 ttctgcatca cctggcatga aagggattac agcgggctta aggtaatttc tacacggcgg
     3601 ttttgcgcct taccttctgc agtgctgttg ctggcgattg ggttagccgg gccaaggccc
     3661 tgagtacgga tgcggctggc gtccacgccc tgggtgatca acgcgctggc aacggaatcc
     3721 gcacgctgct gggagagacg catgttcagg tcgtgcccac ccgtgctgtc ggtataacca
     3781 atcacgttaa ccgccgtttt cggatactct ttcagtacca ttgccacgcc ggtcagggtg
     3841 ttagcgcccg ccggtttcag ggttgcgctg ctgctgtcga aggtcacatt gttcggcata
     3901 ttgaggataa tgttatcccc gctgcgggtt acgctaacac cagtgccgcg cattttgtca
     3961 cgcagcttcg cttcctgcac atccatgtaa taacccacgc cgccgcccag agctgcgcct
     4021 gctgctgcgc caatgagcgc gcctttaccg cgatctttct tcgaagaaga gagcgcacca
     4081 atacccgcgc caacgagaga gcccagacct gcgccgatag cagatttacc tgcttcgcgt
     4141 tcgccggtgt aagggttagt tgtacagcca gataccgcca gagcgccact cactacggcg
     4201 gcaataagat aaacacgttt cttcattgtt aatccttaat aaccttttta ttctttgcca
     4261 cgggttctgt ggcgggagat tatgccgcgt gaacatgaag attattcctg ggaatactcg
     4321 gaaatttgta agtaatattt aactgctcaa tacatctaac ctttcaggag tcttcggttt
     4381 ggccaactca tcctcacgat attccgttct gactgccgcc cactgggggc ctatgctggt
     4441 tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg ttagccacag gaatggaaaa
     4501 ctccttgcag agcgcggttc gcgaccaggt tcacagcaat acgcgggtgc gatttccaat
     4561 ggtgcgcaaa ggctttcttg cgtcaccgga aaatccgcaa ggcattcgtg ggcaggatga
     4621 atttgttcgc gtgagttggg atgaggcgct ggagcttatt caccatcaac ataaacgcat
     4681 tcgtgaggct tatggtccgg catcgatttt tgctggttcc tacggctggc gttcaaacgg
     4741 cgtgctgcat aaggcctcga cattattaca acgctatatg gcgctggcag gcggttatac
     4801 tgggcatctg ggggattatt cgaccggcgc ggcacaggcg atcatgccgt atgtcgtggg
     4861 tggcagcgaa gtttatcaac agcaaaccag ttggccgctg gtgctggaac atagcgatgt
     4921 catggtgctg tggagtgcta acccactcaa tacgctgaaa attgcgtgga atgcatccga
     4981 tgagcaggag ctttcttact tttccgcact gcgtgacagc gggaaaaagc tgatctgcat
     5041 tgatccaatg cgatcggaaa ccatcgattt ctttggcgat aaaatggagt gggtggcacc
     5101 acacatgggc accgatgttg cgttgatgct ggggatcgcc catacgctgg tggaaaatgg
     5161 ttggcacgac gaagcgtttc tggcgcgttg caccacaggt tatgccgtct tcgcctctta
     5221 tttgctgggc gagagtgatg gtatagcgaa aaacgccgaa tgggcggcag agatttgtgg
     5281 tgttggcgca gcgaaaatcc gcgagctggc ggctattttc caccaaaata caaccatgct
     5341 gatggcaggc tggggaatgc agcgccaaca gtttggtgag caaaaacact ggatgctcgt
     5401 cacgctggcg gcaatgttgg ggcaaatcgg cacacccggc ggcggttttg gtctttctta
     5461 ccattttgcc aatggtggta accccacgcg ccgcgctgcg gtgctctctt ccatgcaggg
     5521 cagtttgcct ggcggcaccg atgcggtgga taaaatccct gttgcccgca ttgttgaagc
     5581 actggaaaac cccggtggcg catatcaaca taacggtatg gaccgacatt tcccggatat
     5641 tcgttttatc tggtgggcgg gtggtgccaa ctttactcat catcaggata ccaatcgcct
     5701 gatccgtgcc tggcaaaaac cggagctggt ggtgatctct gaatgcttct ggacggcggc
     5761 ggcgaaacac gcggatatcg ttctgcctgc gaccacctct tttgagcgta atgatctcac
     5821 catgaccggc gattacagta atcagcatct ggtgccgatg aagcaagtgg tgccgccacg
     5881 ctatgaagcg cgtaatgatt ttgatgtttt tgccgagtta agtgaacgtt gggagaaggg
     5941 cggctatgcg cggtttacgg aaggaaaaag tgagctgcaa tggctggaaa cgttttataa
     6001 cgttgcccgg caacgcgggg caagccagca ggttgaattg ccgccatttg ctgagttctg
     6061 gcaagccaac cagttaattg agatgccgga aaacccggac agcgagcggt ttattcgctt
     6121 cgccgatttt cgccgcgatc cgcaggcgca tccattaaaa accgccagcg gcaagattga
     6181 aatcttctcg cagcgtattg ccgattacgc ttatccggat tgccctgggc atccaatgtg
     6241 gctggagccg gacgaatggc agggcaatgc cgaaccggaa cagttgcagg tactttctgc
     6301 tcatccggca catcgtctgc acagccagct gaattacagt tctctgcgcg aattgtacgc
     6361 ggtggcaaac cgtgagcctg tcaccattca tcctgacgat gctcaggcgc gcggcataca
     6421 agatggcgat actgttcggc tgtggaacgc acgcgggcaa attcttgccg gggcagtcat
     6481 tagcgaggga attaaacctg gcgtgatttg cattcacgaa ggggcatggc cggatctgga
     6541 tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac gtgctgacca aagatctccc
     6601 cagctcgcgg ctggggaatg gctgtgcggg taatacggcg ctggcatggc tggaaaaata
     6661 caacggtccg gaactgacac ttacagcgtt tgaaccaccg gccagctcat aatccaggtg
     6721 ggtagttggg tttcatcctg ccatgcgcag tcgacaatgt gaaaaccctg cgcctggtaa
     6781 aaatctatcg ccggttgatt tttttgataa acctccagca tcagatgggg atggcgctgc
     6841 tgcacatact gcattagcgc cttaccaata ccgcgtctga cggccttcgg tgcgacaaac
     6901 attgcagcca gaaatcggcc ttccataatg ctgacaaaac cgagaagctt accgtcttct
     6961 tcccagaccc agttttgcgc gttggcaaga tacgcatcgc gcaccagcgg aatgcagtca
     7021 cgccagtaac tcgattttat aaagggatgc ccccaggttg tactttccag ccacagttcg
     7081 aggatcgcgg ggagttctga atgttgcact tcccgaatca tggtttattt cccggatggc
     7141 aacagcagcc aaccacatga tcattcacca gcccacatgc ctgcataaag gagtaacaga
     7201 ttgtggtacc gacaaactta aaaccacgtt tttttagtgc cttagatagg gcgtcggagg
     7261 cgggtgtaga tgtggggatt tcgctcaacg ttgtggcttg tgtcacctgt ggttgatgat
     7321 tgacaaacga ccagacaaag tcggcaaacg gttcgccgtt ctgttccatt tgcaggtacg
     7381 cccgcgcatt accaataatt gcctgaattt tcccgcgatg gcggataatc ccggcgtcct
     7441 gtaccagtct ttcgacatcc tcttcctgca ttgcagccac cttcaccgga tcgaactgat
     7501 gaaagctggc gcgatagttt tcgcgttttt tgaggacggt gatccacgat aatccagcct
     7561 gctgcccttc aaggcagatc atttcgaaca gttttttact gtcagtttca ggcacgcccc
     7621 actcattatc atggtaggca atataaagcg ggtcctgact cacccagccg caacgttcca
     7681 tactttccct cgctatgtac ccgatcgtaa aaatttcagc cgcttttctt gacgcatcgg
     7741 tgaaaaagtc aatatttaat acagggctac tatgcccgat atccttcata caatgacaat
     7801 aatattgccg aattcggctc ttccctggag tcgtgttaat gatcataaaa aaaagcggtg
     7861 gtcgctggca gctaagcctg ctggcgagtg tggtaatcag tgcctttttt ctcaacacag
     7921 cttacgcctg gcaacaagaa tatatcgttg atacgcaacc cggacattcc acagagcgtt
     7981 acacctggga tagcgatcat caacctgatt acaacgatat tttgtcgcaa cgtattcaaa
     8041 gtagccaaag ggcgctggga ctggaagtca atctggcgga agaaactcct gtggatgtga
     8101 ccagcagtat gagtatgggc tggaattttc ctttatatga gcaggttaca accggcccgg
     8161 tcgcggcatt acattacgat ggcacaacca cctcgatgta taacgagttt ggcgacagta
     8221 ctaccacgct ggccgatccg ttatggcatg ccagcgtgag taccttaggc tggcgtgttg
     8281 actcccggct tggcgatctc cgaccctggg cgcaaatcag ctataaccag caatttggcg
     8341 agaatatctg gaaggcgcaa tcaggcctga gccggatgac ggcgacaaac cagaacggca
     8401 actggctgga tgtcaccgta ggcgctgata tgttgctcaa tcaaaatatt gctgcctatg
     8461 ctgcgctatc tcaggcagaa aataccacta acaatagcga ctatctgtat acgatggggg
     8521 ttagcgccag attttaacgt tatagataca attgaaacca ataaataaca actaatgtgg
     8581 cgatagtggg tgataactta ctaaataata aatttggtga ataattgtcg agtcattcat
     8641 tcctgaacta atgcatttca ttccgttctg atggcatttc atgccgtttt tccccaggca
     8701 taaagtgcac ttcgttatgg ttgtcagcag agatttttcc tttttattac tgcaggaata
     8761 ctgccatgac accttcaaat tatcagcgta cccgctggtt gacactcatc ggtactatca
     8821 ttacccagtt tgcccttggg tcggtttata cctggagcct gtttaacggc gcgctttctg
     8881 ccaagctgga tgcgccggta agccaggtcg ctttctcttt cggcttgtta agtctggggc
     8941 tggcaatttc atcttctgtt gcggggaaat tacaggaacg ttttggcgtt aaacgcgtca
     9001 ctatggcttc cggcattttg ctgggattag gcttcttcct gacagcgcat tctgacaacc
     9061 tgatgatgct gtggttaagc gccggtgtgc tggtcggtct ggcggatggc gcgggttacc
     9121 tgctgacgct ctctaactgc gtgaagtggt tcccggagcg taaaggtctg atctccgcgt
     9181 tcgctatcgg ttcttatggt ctgggtagcc tgggtttcaa atttatcgac acgcagctgc
     9241 tggaaacggt gggtctggaa aaaacctttg tgatttgggg agcgattgcg ctggtgatga
     9301 ttgtctttgg cgcaacatta atgaaagacg caccaaaaca ggaagtgaaa accagcaatg
     9361 gtgtggtgga gaaggactac accctggcag agtcgatgcg taaaccgcag tactggatgt
     9421 tagcggttat gttcctgact gcgtgcatga gtggtctgta tgtgattggt gtagcgaaag
     9481 atatcgctca aagtctggcg catcttgatg caatttccgc agccaatgct gtgacggtta
     9541 tttccatcgc caacctttct ggtcgtctgg tgctgggcat tctgtctgac aaaatcgccc
     9601 gtatccgtgt tatcaccatt ggtcaggtga tatcgctggt gggtatggcg gccctgctgt
     9661 ttgcaccatt gaatgcagtg acgttctttg cagcgattgc ctgcgtggca tttaactttg
     9721 gcggcactat cacggtgttc ccgtcactgg tcagtgagtt cttcggcctc aataacctgg
     9781 cgaaaaacta cggtgtgatt tatctcggtt tcggtatcgg cagcatttgt gggtcgatta
     9841 tcgcctcact gtttggcggc ttctatgtga ccttctacgt gattttcgca ctgctgattc
     9901 tgtcactggc gctctctacg acgattcgtc agccagagca gaaaatgttg cgtgaggcgc
     9961 atggctccct ttaatcatct gccgcgagct actgggcttt agccttagtg tgaatcaaac
    10021 gcttcaacgc aatgtataaa caatgacgcg taaaatttgg gccagttaac tggccctttt
    10081 tactgctttt tgtaacgcta aagtctcttt tcaaacttgc atttttgtaa atttgtgctt
    10141 catgcacact ctttccccac actttttccc tttgctgtgg tctacttatt cgcgcgtgta
    10201 gattttactt atctgactac ctccgcactt ttcccctgcc gggcctgaaa agccgctaag
    10261 cagggtgtta tcacctgttt gtccagggtt tgtttgcatg agatacatca aatcgattac
    10321 acagcagaag ctgagctttt tgcttgcaat ctatattggc ctttttatga atggcgcggt
    10381 tttttaccgc cgcttcggca gctatgcgca cgattttacc gtctggaaag gcatttctgc
    10441 tgttgttgaa ctggccgcca ccgtactggt gaccttcttt ttactacgtc ttctttcgct
    10501 gtttggccgc cgtagctggc gtattctggc gtcgctggtg gtgctctttt ccgcaggtgc
    10561 cagctattac atgaccttcc ttaatgtggt cattggttat ggcatcatcg cttccgtcat
    10621 gaccaccgat atcgacctgt caaaagaagt tgttggtctg aactttattc tctggttaat
    10681 cgccgttagc gcgttgcctc ttatccttat ctggaataac cgctgtcgtt acaccttgct
    10741 ccgacaactg cgaaccccag ggcagcgtat tcgcagcctg gcggtcgtcg tactggcagg
    10801 tattatggtt tgggcaccga ttcgtttgct ggatatccag cagaagaaag tggagagggc
    10861 gacaggtgtt gatttgccga gttatggcgg cgtcgtcgcg aactcttatc tgccatcaaa
    10921 ctggctttcg gcattaggtc tttatgcctg ggcgcgggtc gatgaatctt ccgataacaa
    10981 ttctctcctt aaccctgcga agaagtttac gtaccaggct ccacaaaacg ttgatgatac
    11041 atatgtcgtt ttcattattg gtgaaaccac gcgctgggat catatgggca ttttcggcta
    11101 tgaacgcaat accacaccga agctggccca ggaaaaaaat ctggcggcgt tccgtggtta
    11161 ctcctgtgat accgcaacca aactctctct gcgttgcatg tttgtacgtc aggggggcgc
    11221 ggaagataat ccgcagcgca cattaaaaga acagaacatt ttcgcggtat tgaagcagtt
    11281 aggattcagt tctgatctct acgctatgca aagcgaaatg tggttctaca gcaatacgat
    11341 ggcggacaac attgcttatc gtgagcagat tggtgcggag ccacgtaacc gtggcaaacc
    11401 ggtagatgat atgttgctgg tagacgaaat gcagcaatcg ctggggcgca acccggatgg
    11461 taagcatctg atcattctgc ataccaaagg ctcacacttt aactacaccc agcgttaccc
    11521 gcgcagcttt gcgcagtgga agccggaatg tattggtgtt gatagcggct gtaccaaagc
    11581 gcagatgatc aactcatacg acaactcggt gacctatgtg gatcacttta tttccagcgt
    11641 gattgatcag gttcgcgata agaaagcgat tgtgttctat gcagctgacc atggtgagtc
    11701 aattaatgaa cgcgagcacc tgcacggcac gccgcgtgaa ctggcaccgc cggaacagtt
    11761 ccgcgtgccg atgatggtct ggatgtcgga taaatatctg gaaaatccgg tcaatgcgca
    11821 ggcatttgcg cagctgaaaa aagaagctga catgaaagtg ccacgccgtc acgtagagct
    11881 gtacgacacc atcatgggtt gtcttggcta tacttcaccg gatggtggaa ttaacgaaaa
    11941 caacaactgg tgccacatcc cgcaggcaag agaggcggcg gctaactaag aggcctgctg
    12001 actttctcgc cgatcaaaag gcattttgct attaagggat tgacgagggc gtatctgcgc
    12061 agtaagatgc gccccgcatt cggtgattgg cgcagcctgg tagcgcactt cgttcgggac
    12121 gaaggggtcg gaggttcgaa tcctctatca ccgaccaaat tcgaaaagcc tgctcaacga
    12181 gcaggctttt ttgcatctgt agctcatgag gaagagaacc cccgggggcg gggaggttcg
    12241 actcgacgta ggtctgataa gacgcgccag cgtcgcatca ggcaacggca ccgcacttat
    12301 tgtcggatgc ggcgtgaacg ccttattcga tctacacacc acacatttcc ctgtcaaatt
    12361 tgcgccccat atctcactat ccattaacgg atttgtgaca gaaaccattg tcttttttta
    12421 tatttgctta aatctttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg
    12481 ttttagcgtt catggtatta attgctcaga tatccacctt ttcctcagat ttttttcatt
    12541 aaatgcataa aacttaatca ccaaatgttg ctaaatatta agctgattgt tctggttgtg
    12601 ttggcgtagc acaaatttct ctgctgcaaa tagctgtttg aagttttttt aatctttgtt
    12661 tgtggattct caccgttggt gtaaatcccg gttggctgta ttgacgtttt cacattctgt
    12721 tgacagattg taggtcacga ggggcatttt atggaggatc cgcactgtta cactgatgtt
    12781 aattagtacg gcatccccac ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt
    12841 caggcagcga caacccactg caaagggtta aaacaacaaa catcacaatt ggagcagaat
    12901 aatgcgtatt tccttgaaaa agtcagggat gctgaagctt ggtctcagcc tggtggctat
    12961 gaccgtcgca gcaagtgttc aggctaaaac tctggtttat tgctcagaag gatctccgga
    13021 agggtttaac ccgcagctgt ttacctccgg caccacctat gacgcctctt ccgtaccgct
    13081 ttataaccgt ctggttgaat ttaaaatcgg caccactgaa gtgatcccgg gcctcgctga
    13141 aaagtgggaa gtcagcgaag acggtaaaac ctataccttc catctgcgta aaggtgtgaa
    13201 gtggcacgac aataaagaat tcaaaccgac gcgcgaactg aatgccgatg acgtagtgtt
    13261 ctcgttcgat cgtcagaaaa acgcgcaaaa cccgtaccat aaagtctctg gcggcagcta
    13321 cgaatacttc gaaggcatgg gcttaccaga gctgatcagc gaagtgaaaa aggtggacga
    13381 taacaccgtt cagtttgtgc tgactcgccc ggaagcgccg ttcctcgctg acctggcaat
    13441 ggacttcgcc tctattctgt caaaagaata tgctgacgcg atgatgaaag ccggtacacc
    13501 agaaaaactg gacctcaacc caatcggtac cggcccgttc cagttacagc agtaccagaa
    13561 agattcccgt attcgctata aagcgtttga tggctactgg ggcaccaaac cgaagatcga
    13621 tacgctggtc ttctctatta ctcctgacgc ttccgtgcgt tatgcgaaat tgcagaagaa
    13681 cgaatgccag gtgatgccgt acccgaaccc ggcagatatc gcccgcatga agcaggataa
    13741 atccatcaac ctgatggaaa tgccggggct gaacgtcggt tatctctcgt ataacgtgca
    13801 gaaaaaacca ctcgatgacg tgaaagttcg ccaggctctg acctacgcgg tgaacaaaga
    13861 cgcgatcatc aaagcggttt atcagggcgc gggcgtatca gcgaaaaacc tgatcccgcc
    13921 aaccatgtgg ggctataacg acgacgttca ggactacacc tacgatcctg aaaaagcgaa
    13981 agccttgctg aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt gggcgatgcc
    14041 ggtacaacgt ccgtataacc cgaacgctcg ccgcatggcg gagatgattc aggcagactg
    14101 ggcgaaagtc ggcgtgcagg ccaaaattgt cacctacgaa tggggtgagt acctcaagcg
    14161 tgcgaaagat ggcgagcacc agacggtaat gatgggctgg actggcgata atggggatcc
    14221 ggataacttc ttcgccaccc tgttcagctg cgccgcctct gaacaaggct ccaactactc
    14281 aaaatggtgc tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca
    14341 taacaaacgc gttgaactgt acaagcaagc gcaggtggtg atgcacgatc aggctccggc
    14401 actgatcatc gctcactcca ccgtgtttga accggtacgc aaagaagtta aaggctatgt
    14461 ggttgatcca ttaggcaaac atcacttcga aaacgtctct atcgaataat taaaagccat
    14521 acaagactga tggcaaaggc aaaactgcct gatgcgctcc gcttatcagg cctacgaaaa
    14581 ctctgcaatg tattgagttt gcacgatttt gtaggtcgga taaggcgttt gcgccgcatc
    14641 cggcataaac aaagcgctct ttgtcaacaa tctgcatacc cggtggcgct gtgcctctgc
    14701 gtgcagcgcc atccggcagc aatacttcat tccctgttcc acaagggcgg gaattgattt
    14761 gtgagcaata cagacacgca gttccaggct gcgggtcatt atagagaatc cgggttatgt
    14821 tgcagtttat tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc
    14881 tcacatttgc ctttgtccac atgatcccgg gcgatccggt gatgatcatg gcgggcgagc
    14941 gtgggatctc cccagagcgt cacgcgcagc tgctggctga actcggctta gataaaccga
    15001 tgtggcagca gtatctccat tacatttggg gcgtaatgca cggcgattta ggcatttcga
    15061 tgaaaagccg catcccggta tgggaagagt tcgtgccgcg cttccaggcc acgctggaac
    15121 ttggcgtctg cgcgatgatt tttgctacgg cagtcggtat tccggttggt gtgctggctg
    15181 cggttaaacg cggttccatt ttcgatcaca cggcggttgg cctggcgctg acaggttatt
    15241 caatgcctat cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc
    15301 tgacgcccgt ctccggtcgc gtgagcgaca tggtgttcct cgatgattcc aatccgttaa
    15361 ccggttttat gctgatcgac accgccatct ggggtgaaga cggtaacttt atcgatgccg
    15421 tcgcccatat gatcttgccc gccattgtgc tgggtactat cccgctggcg gtcattgtgc
    15481 gtatgacacg ctcctcgatg ctggaagtgc tgggtgaaga ttacatccgc accgcgcgcg
    15541 ccaaagggtt gacccgcctt agggtgatta tcgtccatgc gctgcgtaac gcgatgctgc
    15601 cggtggtgac cgttatcggc ctgcaggtgg gaacgttgct ggcgggggcg attctgaccg
    15661 aaaccatctt ctcgtggccc ggtctggggc gctggttgat tgacgcactg caacgccgcg
    15721 actatccggt agtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaact
    15781 tgctggtcga tctgctgtac ggcgtggtga acccgcgtat tcgtcataag aagtaagggg
    15841 acatcatgtc acaggttact gaaaataaag tgattagcgc accggtgccg atgaccccgt
    15901 tacaggagtt ctggcactat tttaaacgca acaaaggcgc ggtcgtcggg ctggtttacg
    15961 tcgtcatcgt gctgtttatc gcgatctttg ccaactggat tgcaccctat aacccggcgg
    16021 aacagttccg cgatgcactg ctcgccccgc cagcctggca ggaaggcggc agcatggcgc
    16081 acttgctggg caccgatgac gtaggccgtg atgtgctgtc gcgtctgatg tacggtgcgc
    16141 gattgtcact gctggttggc tgtctggtag ttgtgttatc gctgattatg ggcgttattc
    16201 tcggcctgat cgccggttac ttcggcggcc tggtcgataa catcatcatg cgcgtggtcg
    16261 atatcatgct ggcgctgcca agtctgctgt tggcactggt gctggtggcg attttcggcc
    16321 cgtcgattgg taacgccgcg ctggcgctga cctttgttgc cctgccgcac tatgtgcgct
    16381 taacccgcgc cgccgtgctg gtggaagtta accgcgatta cgttaccgcg tctcgcgtgg
    16441 caggtgccgg ggcgatgcgt cagatgttta ttaacatctt cccgaactgc cttgcgcctc
    16501 tgattgttca ggcgtcgctc ggtttctcta acgccattct cgatatggct gctcttggtt
    16561 tcctcggcat gggggcgcag ccgccaacgc cggagtgggg caccatgctc tccgacgtgt
    16621 tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc gggtctggcg atcctgctga
    16681 cggtgctggc atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactga
    16741 agcagtaaga ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc
    16801 gacgaaagcg caccgttccg cgccgtagac cgcatcagct acagcgtaaa acagggcgaa
    16861 gtggtcggga ttgtgggtga gtccggctct ggtaaatcgg tcagttcgct ggcgattatg
    16921 gggctgattg attatccggg ccgcgtaatg gcggaaaagc tggagtttaa cggtcaggat
    16981 ttgcagcgta tctcggaaaa agagcgccgc aaccttgtgg gtgccgaagt ggcgatgatc
    17041 ttccaggacc cgatgaccag ccttaacccg tgctacaccg tgggtttcca gattatggaa
    17101 gcgattaagg tgcatcaggg cggtaataag agcactcgtc gtcagcgggc gattgacttg
    17161 ttgaatcagg tcggtattcc cgatccggct tcgcgtctgg atgtttaccc gcatcagctt
    17221 tccggcggca tgagccagcg cgtgatgatt gccatggcga ttgcctgtcg gccaaaactg
    17281 ctgattgccg atgaaccaac cactgcgctg gacgtgacca ttcaggcgca aatcattgaa
    17341 ctactgctgg agctacagca gaaagagaac atggcgctgg tgttaattac ccatgacctg
    17401 gcgctggtgg cggaagcggc acataaaatc atcgtgatgt atgccggtca ggtggtggaa
    17461 acgggcgatg cgcacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg
    17521 cgtgcgctgc cggaatttgc tcaggataaa gaacgtctgg cgtcgttgcc aggtgttgtt
    17581 cccggcaagt atgaccgccc gaacggctgc ctgcttaacc cgcgctgccc ctatgccact
    17641 gacagatgtc gcgctgaaga accggcgctg aatatgcttg ctgacgggcg tcagtccaaa
    17701 tgccattacc cacttgatga tgccgggagg ccgacactat gagtacgcaa gaggccacct
    17761 cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg aagaaaggta
    17821 tgttcgcgcc ggaacgtttg gttaaagcgc tggacggcgt ttcgtttaac cttgaacgcg
    17881 gcaaaacgct ggcggtagta ggggaatctg gctgcggtaa atcgaccctc ggtcggttgc
    17941 tgacgatgat cgaaacgccc accggtggtg aactgtatta ccaagggcag gatctgctca
    18001 agcacgatcc gcaggcgcag aagctgcgtc ggcagaaaat ccagattgtc ttccagaacc
    18061 cttacggttc gctgaatcca cgtaaaaaag tcgggcaaat tcttgaggag ccgctgctga
    18121 ttaataccag cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcgaaag
    18181 tcggcctgaa aaccgaacat tatgaccgct atccgcatat gttctccggc ggtcagcgtc
    18241 agcgtatcgc cattgcccga ggtctgatgc tcgacccgga tgtggtgatt gccgatgagc
    18301 cggtttccgc gctggacgtc tcagtgcgcg cgcaggtgct gaatctgatg atggatttgc
    18361 agcaggagtt ggggctgtct tatgtcttta tctcgcacga cctgtcggtg gtagagcaca
    18421 ttgctgatga agtgatggtg atgtacctgg gccgctgcgt ggagaaggga acgaaggacc
    18481 aaatcttcaa taacccgcgt catccgtaca ctcaggcgct actctccgcg acgccgcgcc
    18541 tgaacccgga cgatcgccgc gagcgcatca agctcaccgg tgaactgcca agcccactga
    18601 atccaccgcc gggctgcgcg tttaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc
    18661 agttgcagcc gcagctaaaa gactacggcg gtcaactggt agcttgtttt gctgttgatc
    18721 aggatgaaaa tccgcagcgt taactcacaa aaccggggcc gtgcgctccg gtttactcaa
    18781 tcagcttcaa cagtggcgac aagcatagca aaatgccgta aagcagaatc agccaggctt
    18841 taaacccgcg aagtttttcc agttgtgcga ctttatagat caggaaaaat ggaatgaggc
    18901 acgacacaat gccatataac gggcttccca actgaaagaa caccagcacc gatacacgaa
    18961 acgagaccca aatcgtcaac gtaatgacga taaaagcgca gatcgccaga gtcagcatgc
    19021 gtgagttaat tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa
    19081 tggcctcgtg gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattca
    19141 gcaccgtcga ggtgatatga atgatatgcc cagggatcac ctgcgcggcc agtgccagtg
    19201 ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg
    19261 aaaaggcaaa aaataggatc accgcgatga gcgtgacata actaattcgg tgggtacgca
    19321 gcgcgagccg ggttgccagt actttatccg cttcccgttt gcggtaggca atattcatcg
    19381 ggtttagcac ctgaataaat accgcagaaa agaagcaaaa aggaatagta agcaggacgt
    19441 cgcggaagaa gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga
    19501 tcatcgcaaa accgaagacg acaataatcc ctactttgac caccaccatt ggcccggaaa
    19561 tcttaaacag taatcgttca ccaccagacg caattgccac cagtacggcg aaaatggcga
    19621 ctttataaag tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg
    19681 aggcgctgtc gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa
    19741 agtagataac cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg
    19801 tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc
    19861 aggtggcagg ataagcaatg atcgctgcgg taataaatac ccaaattccc ttcaagccaa
    19921 tttgtactgg catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc
    19981 agccgaagtc gtagcgtgta aacggcattt tctgatcgtg tttcggtagt gtgttgtgct
    20041 gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct
    20101 tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact
    20161 ggggaggtcg cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt
    20221 cagcccggat atgaactgca actaatgcaa ataaagaaat taagctggaa ggcatcacgc
    20281 aatgaggaag attgccgggt gctgacggta aaaagcaatc gtaaaatcag cggcaagccg
    20341 gaaaggttcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca ttggttgctg
    20401 gagagagaaa acccccgcac gttgcaggta tgcacctgac aacatcacgg gggctaatct
    20461 tgaacctaga ccactcaaga atagccgcga aacgttgtca ttacaacaca ggcggctata
    20521 tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg gtcatagctg
    20581 gcattcttgc cagtctgatc gtgaactggc tgaacaagcg gaagtaacgt gtcagacggg
    20641 cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc cgcagggata aaactctgcg
    20701 gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg gaatgcacag
    20761 cacagagtga agaagtgacg gatttcggag ctgaaaaaca gtcgtagaat cagcggcaag
    20821 ccggaaaggt tccggtgagg cgcaatgttg cgggggctta atccctggtg gcattggttg
    20881 ctggagagag aaaacccccg catattgcag gtatgcacct gacaacatca cgggggccat
    20941 tcttgaacct acacaactca agaatagccg cgaaacgttg tcattacaac tcaggcggct
    21001 atatgacgct cgcagagctg ggcatggcct tctggcatga tttagcggct ccggtcattg
    21061 ctggcattct tgccagcatg atcgtgaact ggctgaataa gcggaagtaa cgtgtcagac
    21121 gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg ataaaactct
    21181 gcggcctttt tcgtaagaaa aatgcaacgg ttgcaagcgg ataatattct gcggaatgcg
    21241 cagcacagag tgaagaagtg atggattccg gaacagaaaa acagtcgtag aatcagcggc
    21301 aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc ttaatccctg gtggcattgg
    21361 ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca ccacgggggc
    21421 cattcttgca tctagacca
//
LOCUS       DAZGAP010000087        21068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGAP010000087 DAZGAP010000000
VERSION     DAZGAP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.087"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..1139
                     /locus_tag="ACHLAM_002761"
     CDS             117..1139
                     /locus_tag="ACHLAM_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO7854265.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            1139..2119
                     /locus_tag="ACHLAM_002762"
     CDS             1139..2119
                     /locus_tag="ACHLAM_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO7854266.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVTV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            2116..2874
                     /locus_tag="ACHLAM_002763"
     CDS             2116..2874
                     /locus_tag="ACHLAM_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7854267.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGQ
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            2884..3696
                     /locus_tag="ACHLAM_002764"
     CDS             2884..3696
                     /locus_tag="ACHLAM_002764"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7854268.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            3693..4547
                     /gene="modD"
                     /locus_tag="ACHLAM_002765"
     CDS             3693..4547
                     /gene="modD"
                     /locus_tag="ACHLAM_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO7854269.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            4573..6543
                     /locus_tag="ACHLAM_002766"
     CDS             4573..6543
                     /locus_tag="ACHLAM_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO7854270.1"
                     /translation="MRLKKRYLCTALSLAFTQQALAAQESDTLTVWSSPVSSTTTTVL
                     DHPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(6593..6847)
                     /gene="ymgE"
                     /locus_tag="ACHLAM_002767"
     CDS             complement(6593..6847)
                     /gene="ymgE"
                     /locus_tag="ACHLAM_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7854271.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7048..7782
                     /gene="ycgR"
                     /locus_tag="ACHLAM_002768"
     CDS             7048..7782
                     /gene="ycgR"
                     /locus_tag="ACHLAM_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO7854272.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7784..8395)
                     /gene="emtA"
                     /locus_tag="ACHLAM_002769"
     CDS             complement(7784..8395)
                     /gene="emtA"
                     /locus_tag="ACHLAM_002769"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO7854273.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8495..9409
                     /gene="ldcA"
                     /locus_tag="ACHLAM_002770"
     CDS             8495..9409
                     /gene="ldcA"
                     /locus_tag="ACHLAM_002770"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO7854274.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            9504..11240
                     /gene="cvrA"
                     /locus_tag="ACHLAM_002771"
     CDS             9504..11240
                     /gene="cvrA"
                     /locus_tag="ACHLAM_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO7854275.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11297..12367)
                     /gene="dadX"
                     /locus_tag="ACHLAM_002772"
     CDS             complement(11297..12367)
                     /gene="dadX"
                     /locus_tag="ACHLAM_002772"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO7854276.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYSRVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12377..13675)
                     /gene="dadA"
                     /locus_tag="ACHLAM_002773"
     CDS             complement(12377..13675)
                     /gene="dadA"
                     /locus_tag="ACHLAM_002773"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO7854277.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14005..15537
                     /gene="ycgB"
                     /locus_tag="ACHLAM_002774"
     CDS             14005..15537
                     /gene="ycgB"
                     /locus_tag="ACHLAM_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO7854278.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(15589..16308)
                     /gene="fadR"
                     /locus_tag="ACHLAM_002775"
     CDS             complement(15589..16308)
                     /gene="fadR"
                     /locus_tag="ACHLAM_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO7854279.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            16530..18071
                     /gene="nhaB"
                     /locus_tag="ACHLAM_002776"
     CDS             16530..18071
                     /gene="nhaB"
                     /locus_tag="ACHLAM_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO7854280.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            18217..18747
                     /gene="dsbB"
                     /locus_tag="ACHLAM_002777"
     CDS             18217..18747
                     /gene="dsbB"
                     /locus_tag="ACHLAM_002777"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO7854281.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(18792..20060)
                     /gene="umuC"
                     /locus_tag="ACHLAM_002778"
     CDS             complement(18792..20060)
                     /gene="umuC"
                     /locus_tag="ACHLAM_002778"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO7854282.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(20060..20479)
                     /gene="umuD"
                     /locus_tag="ACHLAM_002779"
     CDS             complement(20060..20479)
                     /gene="umuD"
                     /locus_tag="ACHLAM_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7854283.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            20740..21066
                     /gene="hlyE"
                     /locus_tag="ACHLAM_002780"
                     /pseudo
     CDS             20740..21066
                     /gene="hlyE"
                     /locus_tag="ACHLAM_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         4978 a         5531 c         5335 g         5224 t
ORIGIN      
        1 gttatttaag caacaagcaa atcattccgt ctctttagac aaattttgac atcaaacatg
       61 atctttttcg ctatatgtgt aagtatatag cggttttaat tatcaggata ttacttatgt
      121 cactaaccag acggaggttt acacagattc ttgcgtcgac attgttcctg catcatctgc
      181 cgtcctttgc acaatcagtc aaattctggg cctcactgac gcttcccgaa gcccaaaaca
      241 ttacacgcat cgtcagcgca ggcgcgcccg ccgatttact attgctggct gtcgcgccag
      301 aaaaaatggt cggtttttcc tcttttgatt ttgcccgtca ggcattaatt ccattgccag
      361 agcacattcg ccagttcccc aggctgggac gactcgccgg gcgcgccagt acactctcgc
      421 tggaagggct tatggcgtta catcccgatt tggtcgttga ttgcggcaat acggatgaaa
      481 cctggatctc ccaggcacgg caggttagcg aacagacaca aataccctgg ttattgctta
      541 acgggaaact ggaacaatca gcagaacagt taacaacgct tggcaaaacg ttaggcgaag
      601 agcaccgcgc cgcagaacaa gccaatctcg ccagccgctt cgttggtgaa gctcaggcat
      661 tcgccacctc acccgccgct aatctcagct tttatgctgc gcgcggtcct cgagggctgg
      721 aaacgggctt acagggttcg ttgcataccg aggcggcgga attattaggt ttgcacaacg
      781 tcgcgcaaat agccgatcgc cacggtctga cacaggtttc catggaaaat ctcctgcgct
      841 ggcagccgga tattattctg gttcaggagg ccgttactgc agattttatt cgtcgtgatc
      901 ctctctggca gggcgtgaaa gcggttgcgg aacaacgcat cttattttta agtggcctgc
      961 cctttggctg gctggatgcc ccgccgggaa tcaaccgtct tctgggatta cgcagacttc
     1021 acgcctggct ggatcccgcc atcaatcgcc agtttaaaag tgacatgcag cattacgccc
     1081 aactgttctg gcattgccca ttaagtgacg ccgactatca aaaattggtg gcgagctaat
     1141 gagaaccgta aacggatgta ttttgctgct ggcagctatc agtatcactt ttgccgccgt
     1201 aagtggggct tatcatctcg atatgcagca actgctggcg ctcattcttc gtcaagaaaa
     1261 tgttactgtg caggaacaaa ttgtcttctg gcaaatccgc gtgccgcgta ttcttgctgc
     1321 gctgtttctg ggcgcggcgc tggcaggggc cggaaccacc tatcagggaa tgttccgtaa
     1381 tcccttagtg tcaccggata ttttaggcgt ctccgccggt gccggacttg gtgcctgcgc
     1441 ggcaatcctg tggggattat ccattgttta catccagctg tacgcatttt gcggcgggct
     1501 gatggtggtc gcgggcgtct ggttgattac gcgtcgggtg acccgccacg atccgatctt
     1561 aaccctggtg ctggtaggca ttgcgttagg aacgttgtgc ggagcgggga tatcgctaat
     1621 aaaaacgctg gccgatcctt atacccagtt accgtcaatt accttctggt tgctgggggg
     1681 gctgtccacc gtgaccttac gcgatctctg ctatgctgca ccgatcattc tcataggctc
     1741 cttgcccctg ttttttctcc gctggcgcat gaatttactc acgctttccg acgacgaagc
     1801 ccgttcgctg ggactgaacg tgacacgtct gcgctttgga ttgattgtct gtgccacact
     1861 catcaccgcc agcacggtag caatagcggg cataattggt tgggtcggcc tggtcgttcc
     1921 gcacattgcc cgcttgttga ccggtcataa ccatcagcag ctgttgccca tggcaatgtg
     1981 tactggcgcg attctgctcc tgcttaccga tacgctggca cgcagcattg gaacaacaga
     2041 aatcccactt ggcattctga ccgcttttgt tggcgcaccg tttttcctgt ttttgttgtt
     2101 acgcggaggt cgccagtgac tttgcttgcc gtccgtcatg cgtctctggg ttactcccgc
     2161 catccggtgc tacgcgacgt gtccttcacc ctgtcgcaag gaacaatttg ttgtctgtta
     2221 ggagccaatg gttgcggcaa aaccacactc atgcgctcca ttcttggcgt tataccgtta
     2281 ttaaaaggcg agatcttact ggatagcctc cctgtgcaga cactcagcca tcgccagcgc
     2341 gcgcaggcta ttgcctgggt gccgcaggcg cacgacggca tttttgcttt tagtgtgctg
     2401 gatatggtgt tgatgggcca ggcccccacc ataggcgctt tttccgttcc cggtaagcaa
     2461 gaacgcctga aagcaacgga gcaactggaa aagctgggca ttttgcatct ggcgacacga
     2521 cgctggaata cgcttagcgg cggtgaacgt cagctggctt taatcgcccg tgcactcgtc
     2581 cagcaaccgc gcttgttatt actggatgag cctgcctcta gcctcgattt cggtcatcag
     2641 atccaacttc tggacacgct ggcgcagctc aaaaataacg gcatgacaat gttaatgtcg
     2701 actcatcatc cgctccatgc aaacgccatt gccgacagca ttatccaggt agagccagat
     2761 ggacgagtaa cacaaggctt accgaccgaa cagttaacaa cgaaaaaact ggctgccctg
     2821 taccgggttt ctgccgacca gattcaccat cacctctctg caattaagca ctaaggaaaa
     2881 atcatgctca ttgacgatat tgattttgca gacctttacc ttcagcaact caggctggcc
     2941 catcgcacag aaaaaacgcc ggatcactgg gatcaacggg cggaaaaaat ggcggaaaat
     3001 tgcgcctcgc cgacggacag ctacctgcaa caactgacct caaaaattga cctgcaaggc
     3061 gcgcaaacct tattcgatat gggctgcgga ccgggtaccg tatcgctggc gctggcggac
     3121 aaactcacga cgatttatgg cgttgattac agtcagggga tgctgaacgt tgccgcccgt
     3181 cgcgccgccg cgctaaaggc agacaatgtc cactggatcc agcgcgcctg ggaagaggac
     3241 tggagtgacc tgccacgctg cgatattgcc gtggcttccc gctccactct ggtggctgat
     3301 atgcgccagg caatgagcaa actgaataat caggcacggc ttcgcgtcta tacgactcat
     3361 ctggtcagca cctcgttcgt ctctccagcc attcagcggg cggctggtcg ggaagtggtc
     3421 gaattaccca actatatctt tgcgctcaac gtcctgtatc agatgggaat ttatgctcac
     3481 gttgatttta tccgcggaca aaattgccaa caggataaca gcacctggga acgttttgag
     3541 cagaacgtca gctggagtct gggcgcgctt aacgacgatg agcgtgagcg gttatatcgc
     3601 tggtatcaac aacaagatgc tcgtgccctc gcccctgcca gccgcgactg ggcgctcatc
     3661 tggtgggata gcgtaccgca agaggcactg cgatgatttt cctttctcag gcacaaatcg
     3721 atgcgttact tctggaagat atccagggcg gtgacctgac cacgcgagca ttaaatattg
     3781 gacaccagca tggctatata gagttttttc accgtcaggg cggctgcgtc agcggaattt
     3841 ctgtcgcgtg taagatgtta actacgctgg gattaactat tgatgacgca gtcagcgacg
     3901 gttcacaagc gaacgcgggt cagcggctaa tccgtgcgca gggtaatgcc gcagcacttc
     3961 atcagggatg gaaggcagtc cagaatgtgc tggagtggag ttgcggtgtt tctgattatc
     4021 tcgctcaaat gctggcgtta cttcgtgaac gttaccctga tggcaatatc gcctgcaccc
     4081 gaaaagcaat tccgggcacc cgcttactgg cctcgcaggc aattctggct gccggaggac
     4141 tgattcatcg tgccggatgt gcggaaacca tattattgtt tgccaaccac cgtcattttc
     4201 ttcatgacaa tcaggactgg tcaggcgcaa tcaatcagtt acgccgccac gcacccgaga
     4261 agaaaatagt tgttgaagcc gacacgccga aagaggcaat cgccgcgtta cgcgcgcaac
     4321 cagacgtact tcagctcgac aaatttaatc cgcaacaggc aactgaaatt gcgcaaatag
     4381 caccgtcgct ggctcctcac tgcacgctgg ctctgaccgg cggaattaat ctgacaacac
     4441 tcaaaaatta cctcgactgc ggcattcgcc tttttattac ctccgcgcct tattacgcga
     4501 caccagctga cattaaagtc agtctgcaac ccgcagcctc tatttaattc tttgttaaac
     4561 ggaatttatt ctatgagact caaaaaacgt tatctctgca cagcattatc acttgccttt
     4621 acccagcagg ccttagcggc tcaggagagt gacacgctga ccgtatggtc cagtccggta
     4681 tcatcgacga cgaccaccgt tctcgatcac cccaccatga aggccctgga taaacagaat
     4741 gtcgctcagg cattaagtgt cgtccccggc gtggtgctgc aaaagtcagg tagccgcaac
     4801 gaagaacagg tcaaagttcg tggctttgat agtcgtcagg tgccagtcta tttcgacggt
     4861 gtgcccattt atgttcccta tgacggcaac ctcgatctgg cgcggattct gaccaacaat
     4921 ttgggggcag ttgaagtttc caaagggtat tcgtcgctgc ttcaggggcc taatcagatg
     4981 ggcggagcca ttaatatcac cacccagaag ccaacaaaac ctctggaagc aaatctggga
     5041 tatcgccagg gatggagccg tagccaggac aatgcctacg atatgcatgc ttcatttgcc
     5101 gccagcagcg atctggggta tttgcaagtc agcggtagcc agctaaagca ggattttctc
     5161 ggcctgccgc atggtgtaaa taatgatatt gcaggcaaac acggcaagat gattaattca
     5221 tcggctgatg ataaacgcgg cattgtgaag ctcggtttta caccacgcga aaacgatgaa
     5281 tacacattga cttacattaa gcaggatggt gaaaaagata atccgccata cagtggaaat
     5341 agtggtcaaa aatcacgcta ctggcagtgg ccagagtatg acaaagaaag tttttattat
     5401 cagggaacga cccaactaaa cgatcgtttt accctgaaaa gtcggctgta tcgcgatacc
     5461 tttgaaaata cgctgatgat gtacaactcg ctggctgatt tgaaaaataa aaaaggcagc
     5521 tacagccatt attccgatta cagcgacggt gccgggttac aactggcagc cgatgtgcgt
     5581 gaaaacgatc tgctgtcgtt tgccgttaac tggaaagatg acgtacaccg ggaaaaaggt
     5641 gcgccgcacg ccgcttacga tcgctatgaa gatcgtacct ggtcgctcgc cagtgaatat
     5701 caatgggctg ctgccgataa tgtcgatgtc gtggctggaa tcagctatga ctggcgcgat
     5761 agcgtagaag cgaaaaaaca tgagaaagat ggaagcatca cccattatga cgacaacaat
     5821 cagtcagctt ttaactggca ggtgatgggg aaataccact ttgccaatga agacacgctg
     5881 gcgctttcgt actatgaccg cacacgcttt ccgacgctga aagaacgcta taccacgtcc
     5941 aaacctgcgt ataaccagat agcgattgtt aacccgcagc tcaaaccgga acgcgcgcgc
     6001 ggggtggatt taacctggaa tggtgccttc acgcacgact gggggtttga ggtcagcgtt
     6061 tactataacc gggtgagtga tgccatcctc tcgcacaata tcgatgccga taccattcaa
     6121 aatcagaaca gcggcacggt ggattacagc ggtctggatg ccggtattaa ggggaaaatc
     6181 agcaatatac tggatgtagg attgagctac gccctgatcc acgctgatgc caaacgcaaa
     6241 gacatcggca agataaccga tctgccaacg cagacaatga ccgcatggat gactctcaaa
     6301 ccgtgggagc cgttaagcgt aacgctgtcg gaagaggcgc gttcctccag ctacagcaac
     6361 agtgacggtt cacaaaaagc cgccggtttt gcggtgaccc acattcgagc cgattacacc
     6421 ttaggtcatg gcttcagcgt caatgcgtcg gtcaataacc tgtttgatac caaatatgcc
     6481 tacagtgaag ggtttattga agaaggtcgc aatttctggg caggtatcga gtatacgttc
     6541 tgatgtgaaa gcggatagcg gcatctgtat cgctatccgc cttatgaaaa tcttattctc
     6601 ttcgcaggag gcggaatacg cccagcacca ggatagcgcc cactaccgcc accaggaagc
     6661 tgtgcaaatt aaaaccgctg atggagccgc caatgccaaa catggtcgcc agccagccgc
     6721 cgaccaccgc accgactatc ccaagaatac aggtcaggaa aaatccacca ccatcacgcc
     6781 ccggcatgat tagcttggcg ataatgccgg ctatcaggcc aaaaataatc caggcaataa
     6841 ttcccatgtt caggccctcg tttcgatgaa tcggacgcaa gaaatgctta cgtcaacgct
     6901 ctaagtatag gcaagggcgg gatcgatgct gggcgtatgt cggctgattt aagaacgaca
     6961 tgtaaaaaat attcacgcat ttgtattaag ttttgttaac tgtgaccgat aaacctaaga
     7021 cagtttgtca gtcaggagtt tttccgcgtg agtcattacc atgagcagtt cctgaaacaa
     7081 aatccgttag ccgtcctggg cgtgttacgc gatttgcaca aagccgcaat tcctttgcgt
     7141 atcagttgga atggcgggca gctgatcagc aaaatactgg caataacccc ggataaactg
     7201 gtgctggatt tcggcagtca agccgaagat aacaacgctg tgttaaaggc gcagcacatt
     7261 accattaccg ccgaaaccca gggtgcgaaa gtcgagttta ctgttgaaca actacagcag
     7321 agtgaatact tgcagcttcc ggcatttatt accgtaccgc ctcccacctt gtggtttgta
     7381 caacgacgcc gatatttccg catctccgcc ccactccatc cgccatattt ttgccagacc
     7441 aaactggcgg ataacagtac gttacgtttc cgcctgtatg atttgtcgtt aggcggcatg
     7501 ggcgcattac tggaaacagc aaagcctgcc ggattgcacg aaggcatgcg cttcgctcag
     7561 attgaagtca acatggggca atggggtgtt tttcactttg acgcccagtt aatctccatc
     7621 agcgagcgca aagtgattga tggcaagaat gaaaccatca ccactccccg tctgagcttc
     7681 cgttttctta acgtcagccc gacggtggag cggcaattac agcggattat tttctctctc
     7741 gagcgagaag cccgggagaa agcggacaaa gtgcgcgact gatttacatc gcgtccagtg
     7801 cctgctcaag tttgtagata tagcgcggag cctgcggcgc aggatgattt cgcgctacgt
     7861 gatcgaggaa ctcgtcagca tccagatcgt tgattttgct gatcgccttt ttccgatctg
     7921 acgagaaagt ccgtagcagc gcacctgccc cgttagcata tgacaccacc agcgcatatt
     7981 gcagtacctt cggatcttca atgcctgcca gcgggccggt ttccagaata ttcaggtaag
     8041 ccgcccccat tgaaatatta cgctccggat ttttcagctc gctggtcgtc ggctcaccac
     8101 tccagcccat acggcgataa acatcacgtc cggaggttga agcttttaac tgcatcaaac
     8161 caatggcatt cgatttactc accgcgttcg gattaccacc cgattcgata gcgataatcg
     8221 ccgtgatcaa ttgtggatcg acgccccagg ctgcaccggc tttctggctt attggcatcc
     8281 actgcatcgc acgttgcacc ggaactttcg cgttccacgg cgggttcgta tagtcatgct
     8341 tcgatgaaca acccgctaat aacacaatca aaaaggcaaa ccatctcaat ttcacttctt
     8401 ctatccttat agcctggaat cgccgttgag gtgacaacgg cacacctaag cggcatgata
     8461 tacatttcgt ttctgttgtc agcaaggaat tgccatgtct ctgtttcact taattgcccc
     8521 atcgggttac tgcattaaac agcacgccgc gctgcgtggt atccagcgcc ttaccgacgc
     8581 ggggcatcag gtcaataacg tcgaggttat tgcccgtcgt tgtgagcgtt ttgcgggtac
     8641 ggaaacggag cgtctggaag atctcaattc tcttgccaga ctgactaccc ccaacaccat
     8701 cgtgctggct gtacgcggcg gttacggtgc cagtcgttta ctggcagata ttgactggca
     8761 ggcactggtg gctcgccaac aacatgaccc gttgctcatt tgcggacata gcgattttac
     8821 cgccattcag tgcggtcttc tggcgcatgg caatgtcatc acctttagcg gccccatgct
     8881 ggtagcaaac tttggcgcgg atgaactgaa cgcctttacc gagcaccatt tctggctggc
     8941 gttacgcaat gaaaccttca ccattgaatg gcaaggggaa ggcccgacat gtcagaccga
     9001 aggcacgctg tggggaggca atctcgcgat gctgatttca ctgattggta caccgtggat
     9061 gccaaaaatt gagaacggta ttctggtgct ggaagatatt aacgagcacc ctttccgcgt
     9121 cgaacgtatg ctgttgcagc tctatcatgc tggaattttg ccacgtcaga aggcgattat
     9181 tctcggtagc tttagcggca gcacgcctaa tgattatgac gcaggttaca acctggagtc
     9241 agtgtacgcg tttttgcgtt cccgcctgtc gattccactc attaccggcc tcgattttgg
     9301 tcatgaacaa cgcacggtta cactgccgtt gggcgcacat gccattctga ataacaccca
     9361 ggaaggcacc cagttgacga ttagcggtca tcctgttctt aaaatgtaaa aaaatcacgc
     9421 ttcagggcta agtaaaacgc tgtctctgcc gctaatatgg tagggtttat ataagagaca
     9481 gcgtaatcag gagtaaccga ccgttggatg ccacaacaat aattagcctt ttcatcttag
     9541 gatccatcct tgtcaccagc agtattttac ttagttcatt ttcttcccgt cttggcattc
     9601 ccattctggt tatttttctg gcgatcggca tgctggcggg ggttgatggc gtcggcggta
     9661 tcccgtttga taattatccc ttcgcctaca tggtaagtaa tctggcgctg gcgattattt
     9721 tgctcgacgg cgggatgcgc actcaggcca gttcctttcg tgtggcgtta ggaccggcac
     9781 tgtcgctggc gacgctgggc gtgcttatca cctctggttt aaccggcatg atggcggcgt
     9841 ggctgtttaa tcttgatttg attgaaggat tattaatcgg cgctatcgtc ggctccaccg
     9901 atgctgcagc ggtcttttct ctgctgggtg gtaaggggct taacgaacgt gttggctcga
     9961 cgctggaaat tgaatccggc agtaatgatc caatggcggt ctttctgacg attaccctga
    10021 ttgcgatgat ccagcagcat gaaagcagtg tcagctggat gttcgtggtc gatattttgc
    10081 agcaatttgg cctcgggatt gtcatcggtc ttggcggtgg ttatttactg ctgcaaatga
    10141 ttaatcgcat cgccctgccc gccggtttat atccattgct ggcattaagc ggcggtattt
    10201 taatttttgc tttaaccacg gcgctggaag gcagcggtat tctggctgtt tatctgtgcg
    10261 gttttctgct gggtaatcgg ccgattcgca accgctacgg cattctgcaa aatttcgacg
    10321 gcctcgcctg gctggcgcaa atcgccatgt tcctggtgct ggggctgttg gttaacccaa
    10381 gcgatctgct gcctattgcc attccggcgc tcattttgtc cgcatggatg attttcttcg
    10441 cccgtcctct ttcggtattt gccggattgc taccgttccg tggcttcaat ctgcgtgagc
    10501 gcgtgtttat cagctgggtg ggattacgcg gcgcggtgcc aatcatcctg gccgtgttcc
    10561 cgatgatggc ggggctggag aatgcgcgac tgttctttaa tgtcgccttc tttgtggttc
    10621 tggtttcact actcttgcag gggacatcac tctcgtgggc ggcgaaaaaa gccaaagtgg
    10681 tcgttccgcc agtgggacgt ccggtgtcac gcgttggtct ggatattcat ccggaaaatc
    10741 cgtgggagca gtttgtttat cagttgagtg ccgataaatg gtgcgtgggc gcggcactgc
    10801 gcgatctgca tatgccaaaa gagacgcgta ttgcggcact gtttcgtgat aaccagttgc
    10861 ttcatcctac cggcagcacc cgactgcgcg aaggcgatgt gttgtgtgta attggtcggg
    10921 aacgcgatct cccggcgctc ggtaaactgt tcagccagtc gccaccggtc gcgctagatc
    10981 aacgcttctt tggtgacttc attctcgaag ccagtgccaa atatgctgat gtggcgctga
    11041 tatatggcct ggaagacggg cgagaatatc gtgataagca gcaaacgctg ggtgaaattg
    11101 tccagcagtt gttaggcgcg gcaccggttg tcggtgatca ggtagaattt gccggaatga
    11161 tctggacggt ggccgagaaa gaggataatg aagtgttgaa gattggtgtt cgggtagcgg
    11221 aggaagaagc cgaatcttaa gtttaatgtg ttttgtaacg ctggatacga cgctggtcgc
    11281 atccagcgat aacaagttac actgtcacaa ccgggacacg taacgccagc gcgcacatca
    11341 actcatagcc caccgttccg gcagcggcgg cgacatcatc aattttgatc tccttgcccc
    11401 acagctcaac cggcgtacca atccccgcct gcgggcaagg cgttaaatca accgccagca
    11461 tatccatcga gacggtcccc accgtcatgg tgcgcacgcc gtccactaaa acaggagtac
    11521 cggtaggcgc atggcgcgga taaccgtcgg cgtatcctgc ggcgacaatg ccgattcgct
    11581 gttcatcgcg cgcagtatag cgaccgccgt agcccacacg ctcgcccgct tttagcgtct
    11641 ggacaccaat aatctcactg cttagcgtca tcaccggacg taatccggta ttggcgatat
    11701 cacgccactg accggacggc gaagcgccat acaaaataat gccaggccga acccagtcaa
    11761 aatgcgcttc cgggtgccac agagtcgccg ccgaattgga caacgaacgc cgacactcca
    11821 gcccttccgc cgcctgctca atacgcgcca tcgcgctgga aattccatca ggatgttccg
    11881 cctcggcaaa atgcgacatc agggtcattt cgccaacatt cgccatcgcc cgcaactgct
    11941 gccagacggt aagcacgcga tcgggctgga agcccaaccg attcatccca ctgttcactt
    12001 taagataaat atccaacggt gcttttagcc gcgcattttg cagtgctttg agctgccagt
    12061 tgctgtgtac gcaggtggtc aggcggtgct ggtcataaat ctccagatcc tgagcatgga
    12121 aaaatccttc cagcatcagg atcggtcctt tccagccgcg ctcacgtaac gttattgcct
    12181 cttccagatt aagcaatgca aagccatcgg tggccccgag cgcgctccag atacgctcaa
    12241 taccgtgccc gtaagcgttt gcttttacca ccgaccagac gcgcgagtac ggcgcagcct
    12301 ggcggacaat ggacagattc tgttttaatg cctgcagatc gaggctggcc tgtatcggac
    12361 gggtcatctc gtttccttag ctgtgtgcgc catgtaaatg gcccgggcgt gatggcgtaa
    12421 atccacggct gtagcgcgct acgcttagat cttcatacgg gatcgctggc gtgcgccccg
    12481 agagcagatc gcttaacaac tgaccggaac cgcaagccat cgtccagccg agcgtgccgt
    12541 gaccggtatt cagccacaga tttttaaagc gtgtacgccc gacaaccggc gtgccgtctg
    12601 gcgtcatcgg gcgcaggcca gtccagaaag tcgcctgctc aacatgaccg ccgcgtggat
    12661 agagatcgcg aaccaccatc tccagcgttt cacgacgcgg ttgcaacagc tcggtgttaa
    12721 aaccaacaat ttccgccatt ccgccgacac gaatgcggtt atcgaaacgg gtaatggcga
    12781 ttttgtaggt ttcatcaaga atagtggata ccggcgcacc atcttcctgc gcaattggaa
    12841 tggtcagcga gtagcctttc agcggataca ccggaatatc aacaatgcct ttgagcatcg
    12901 ccgtcgagta agaaccaaac gccatcacat acgcatcggc cttaatcact tcatcaccac
    12961 acttcacgcc gtagatttgc tcgccgtcgc aaagcagttg gtcaacgggc gtattaaagc
    13021 ggaatttaac ccccgcctgc tccgccatcc gcgccagatt ctgggtaaac agctggcagt
    13081 ctccggtttc atcattgggc agttgcaggc cgcccgtcag tttgtgcgcc acttctgcca
    13141 gcgcgggctc cacttccgcc aggcggctgg attccagcag ctgatacggt acgccggcat
    13201 cttccagcac ggcgatatcg cgggtcgcgt tttcatactg ttgttcggta cggaacagtt
    13261 gcagcgtccc accctgacgc ccttcatact gaatattggt ttcggcgcgc aatgctttca
    13321 ggcaatcacg gctgtattcc gccagacgta ccatccgccc tttgttttcc atgtagtggc
    13381 tggtgtcgca gttacgtaac atttgccaca tccatttcaa ctggaactgc gtaccgtcga
    13441 gacgaaccgc cagcggcgca tggcgctgga acatccattt aatcgctttt aaaggcacac
    13501 ctggtgccgc ccacggtgca gcatatccgg gggagatttg cccggcattc gcggcactgg
    13561 tttccagggc ggcccccggc tcccgatcaa tgacggtgac ctcatgtcct gcctgattta
    13621 agtaccaggc gctggcaacg cctaccacac cacttcccag tatgacaact cgcatagcca
    13681 ctccgataac ggtagataca gtaaaagaat aataatctaa ttacatcttg ataactcagt
    13741 tgaaaatatt attcaacata cggctttttt atggtgagct ggctcacatc tccctgactc
    13801 tgcggcaata tggcgtcttt agtatctcct gctgaacgcc ctttttatcc agcataaaat
    13861 tccgttcaga agcggattag tggcactctg ataataagaa atcgcaaaga aaaaaattct
    13921 ccggcgacgc ggtttttaac aggtgttcta tgcttgaaat gaggtgctcc acacagagag
    13981 cgccgacaac aatgaggtgc gcgtatggcg acgatcgatt ctatgaataa ggacaccaca
    14041 cgtttgagcg atggacccga ctggacgttc gacctgctgg atgtttatct ggcagagata
    14101 gaccgggtgg cgaaactcta ccggctggat acctacccgc accagattga agtgataacc
    14161 tcagaacaga tgatggatgc ctactccagc gtcggcatgc caattaacta tccgcactgg
    14221 tcattcggta aaaagtttat cgagactgaa cggctgtata agcacggtca gcaaggactg
    14281 gcctatgaaa tcgtcattaa ctctaacccg tgtatcgctt acctgatgga agagaacacc
    14341 attaccatgc aagcgctggt gatggctcat gcctgctatg ggcataactc tttcttcaaa
    14401 aacaattact tattccgtag ctggaccgac gccagttcga ttgtcgatta tctgattttt
    14461 gcccgtaaat atattaccga gtgcgaagaa cgttatggcg ttgatgaagt agaacggctt
    14521 ctggactcgt gccacgcgct gatgaactac ggcgtggacc ggtacaaacg cccgcaaaaa
    14581 atctcgctgc aagaagagaa agcccggcag aagagtcgcg aagagtatct gcaaagtcag
    14641 gtcaatatgc tctggcgtac cctgccgaag cgcgaggaag agaaaacggt tgctgaagcg
    14701 cgccgctatc cgtccgaacc acaagaaaac ctgctctatt ttatggagaa aaatgcgcca
    14761 ctgctggaat catggcagcg tgaaatcctg cgtattgtgc gtaaggtgag ccagtatttt
    14821 tatccgcaaa aacagactca ggtgatgaac gaaggctggg cgaccttctg gcactacacc
    14881 atccttaacc atctgtatga tgaagggaaa gtaacggaac gttttatgct ggagtttttg
    14941 cacagccaca ccaatgtggt cttccaaccg ccctataaca gcccgtggta cagcggcatc
    15001 aacccatatg ccctcgggtt cgccatgttc caggatatta aacggatttg tcagtcgcca
    15061 acggaagaag acaaatactg gttcccggat atcgccggtt ccgactggct ggaaacgctg
    15121 catttcgcga tgcgtgattt caaagatgag agttttatca gccagttcct gtcaccgaaa
    15181 gtgatgcgtg atttccgctt cttcaccgtg ctggatgacg atcggcataa ttatctggag
    15241 atttccgcta ttcataatga agaaggttat cgggagatcc gtaaccggtt atcgtctcaa
    15301 tataatttaa gtaatctgga gccgaatatt cagatctgga acgtggattt gcgcggcgac
    15361 cgttcgctga cgctgcgtta cattccacat aatcgcgcac cgctggatcg ggggcgcaaa
    15421 gaagtgctga agcatgtgca tcgcctgtgg ggatttgatg tgatgctgga acagcaaaac
    15481 gaagacggca gcgtcgagtt gctggaacgt tgcccgccga gaatgggaaa tctgtaaaaa
    15541 caacgaaaaa cccctcgttt gaggggtttg ctttttaaac ggaagggatt atcgtccctg
    15601 aatggctaaa tcacccggca gatttttctg catccggtgc caaatctcgc cactctcatg
    15661 cccatagcga cgcactgttt cgtatacctg atcgtgtgcg ccttcactgc acaacgccga
    15721 cagtttgtga tagaagccca gcgccagact gcgcgcttcc ggattggcga aatagtgacg
    15781 accaatgcgc gtatacagcc ctttcatccc gttaaggatc agaccgtaaa tcgggttgcc
    15841 ggaagcaaac gccaggccgc ggaaaatgtt gtaatccagc tcggcaaagg cgtcggcgtg
    15901 atcggccact tcattagcgg ttgccagcac ttcctgcgct ttatcgggat gttgacgaaa
    15961 cgcggtgcga ataaaaatag ttgaaatatt ggtacgcacc gacagcaaat tatcaataag
    16021 ctgcggcaca ctttcgtggt ccagtcgcgc cagtgtttca aggatattta aaccagaggt
    16081 ttcccagaaa ttattcacct tcgtcggctt gccgtgttga atggtcaacc agccatctcg
    16141 tgccagacgt tgtaacactt cacgtaacgt agtacgcgtt acgccaatta attctgaaag
    16201 ttcacgttct gcgggcaaaa tagtcccggg agggaagcgg ttattccaga tactttcaat
    16261 aatgtactct tccgcgaaac ccgccgggct ttgcgcctta atgaccatag tgagatttcc
    16321 ataacacagc aaaacaaagt tggactcatc ataccagagg gctaactatg ctgatagaag
    16381 acaaaaagaa gaaaaaaggg atcaaggctt cattttctgc atctctttcc ccttgactat
    16441 caagacttgc ctcactcctg tctgaccgtt aagctttgcg tttaaacata aaaacatcat
    16501 tgattatttc taaggtaagg gaaaccatca tggagatctc ctggggccgc gcactatggc
    16561 gcaatttttt gggccagtcc cccgactggt acaaactcgc cctcatcatt ttcttaatcg
    16621 taaatccgtt aattttcctc atcagccctt tcgtcgctgg ctggttgctg gtagcggaat
    16681 ttattttcac tctggcgatg gccctgaaat gctacccgct gctccccggt ggtctgttgg
    16741 ctatcgaagc ggtattcatc ggcatgacca gcgcggaaca cgtccgtgaa gaggtggcgg
    16801 caaatcttga agtcttgctg ttactgatgt ttatggtggc gggtatctat tttatgaaac
    16861 agctattgct gttcatattt acccgtttgc tgttaagcat tcgctccaaa atgctgctgt
    16921 cgctctcttt ttgcgtggcg gcggcgttcc tctccgcgtt cctcgatgcc ttaaccgtcg
    16981 tggcggtggt gatcagcgtt gcagtcggtt tttatggtat ttatcaccgc gtagcctctt
    17041 cccgtaccga agataccgac ctgcaagacg atagtcatat cgacaagcat tacaaagtgg
    17101 ttctggaaca gttccgtggc tttctgcgta gcctgatgat gcatgccggt gtcggcaccg
    17161 cattaggcgg cgtgatgacc atggtgggcg aaccacagaa cctgatcatc gctaaagcgg
    17221 ctggctggca ttttggcgat ttcttcctgc gcatgtcgcc agtgaccgtt ccggttctga
    17281 tttgtggcct gttaacctgc ctgctggtag agaagctgcg ttggtttggc tatggtgaaa
    17341 cgctgccgga gaaagtccgc gaagtactgc aacagtttga cgatcaaagc cgccaccagc
    17401 gtacccgtca ggataaaatc cgtctgattg tccaggcgat tattggcgtc tggctggtga
    17461 ctgcgctggc gttgcatctg gcggaagttg gcttgattgg tttgtcggtc attattctgg
    17521 cgacatcatt gaccggtgtc accgatgaac atgctatcgg taaagccttc accgagtctc
    17581 tgccattcac cgcactgttg acggtgtttt tctcggtagt cgcggtgatt atcgaccaac
    17641 aactgttttc gccgattatt cagtttgtgt tgcaggcatc ggaacatgct cagctgtcgc
    17701 tgttctatat tttcaacggt ctgctgtcat ccatttcgga taacgtcttc gtggggacga
    17761 tttatatcaa cgaagcgaaa gcggcaatgg aaagtggcgc tatcacgttg aagcaatacg
    17821 agctgctggc ggtcgccatt aataccggta ccaatctgcc ctccgtcgct acgccgaacg
    17881 gtcaggctgc gttcctgttc ctgctgacct ctgcactcgc gccattgatt cgcctctctt
    17941 atggccgcat ggtgtggatg gccctgcctt acaccctcgt cctgacactc gtcggcttgc
    18001 tctgcgtcga gtttacgctt gcccctgtaa ccgaatggtt tatgcaaatg ggctggatag
    18061 caacgctttg ataacaactt accgggcata taaatgcccg gtttgccttt tcgcccgata
    18121 attgtccaat tgcgtcctat ttttgctgcc tcctggtggc gcagcgaatg aattggttta
    18181 aactgcgcac tctatgcata ttgcagggaa atgattatgt tgcgattttt gaaccaatgt
    18241 tcacaaggcc ggggcgcgtg gctgttgatg gcgtttaccg ctctagcact ggaactgacg
    18301 gcactgtggt tccagcatgt gatgttactg aaaccttgcg tgctctgtat ttatgaacgc
    18361 tgcgcattat tcggcgttct gggtgctgcg ctgattggcg cgatcgcccc gaaaactccg
    18421 ctgcgttatg tagcgatggt tatctggttg tatagtgcgt tccgcggtgt gcagttaact
    18481 tacgagcaca ccatgcttca gctctatcct tcgccgtttg cgacctgtga ttttatggct
    18541 cgtttcccgg aatggctgcc gctggataaa tgggtgccgc aagtgtttgt cgcctctggc
    18601 gattgcgccg agcgtcagtg ggaattttta ggtctggaaa tgccgcagtg gctgctcggt
    18661 atttttatcg cttacctgat tgtcgcggtg ctggtggtga tttcccagcc gtttaaagcg
    18721 aaaaaacgtg acctgttcgg tcgctaatcc attcggcgct cctgcgggag cgctttttcc
    18781 tgccgctatg tttatttgac ccgcagtaaa tcagaacttc gcgttgtata acatggtgaa
    18841 agcatggcac gcttcatctg ccattgttgc tggatcccct gcccggcaaa atagagtgtt
    18901 cctctgccct ctttagcatt cagcgtatcc attaccgcca tcaattgctc actcccgggg
    18961 cgcggtgcgt tgtcatcgaa taaattgagc tgcgcgactc cctgactgaa gaaatccccc
    19021 agcatcacgc ccgctttttg gtagcgatgg cccgcttgcc agatggcatc cagagatcgc
    19081 gtagcggcgt taatgatatc cctgctgtcc tgagtgggcg tcagcagttt taccgatgcg
    19141 ctattgccgt aataaggttc attgaacgca aatggtgacg tcttaataaa cgtggagata
    19201 aaccgacaat attgatgctc gctgcgaagt ttttccgccg cccgggcagc gtaactacaa
    19261 atggcctgcc gcatcgacgg ataatccgtg atgcgttcac caaacgagcg ggaacagata
    19321 atttcctgct tcgtcggtgc aaactcttcc agttgcaaac agggttcacc gcgcagttca
    19381 cgcaccgttc tttcgagcac gacattaaaa tgtttacgga taaaccggat atctgaatcc
    19441 gccaaatcga gaacggtttt gatccccatc gcgtccagtt ttttgctgat ccgccgtcca
    19501 atcccccaga cttcatccac gggaagagca gacattaatt tacgctggcg ttccagattt
    19561 gataaatcca ccaccccgcc cgtctgccgc tgccattttt ttgccgcatg attggcaagc
    19621 ttagccagcg ttttggtctg ggcgatcccc acaccaacag taagatgggt acgttgtagc
    19681 accgttgcgc gaatttctct gccaaaatca gtcagatcgc gacaattatg cacaccggtc
    19741 agatcgcaga atgcctcatc aatactgtaa atctcgacgc ggggcgatag ttcttccagc
    19801 gtcgacatca cccgattact catgtctgcg taaagctcat aattgctgct aaagcaaacc
    19861 acgccacagc gacgaaacag atctttttgt ttgaaccagg gatcgcccat tttaacgccc
    19921 agcgcctttg cctcagcgtt tcgggcgata acgcaaccgt cattattcga tagcacaacc
    19981 accggtttac cccataaatc agggcgaaac accgtctcac agctggcata aaacgcgttt
    20041 acatcacaga gggcaaacat cagcgcatcg ccttaacgac gtggatcacc acaccaaaga
    20101 catccagcgt atcttcgcta ctgatggtaa tgggcgagta cgcactgttc atgggaataa
    20161 gctgcactgt cgggcgtagt tgcaattttt tcaccgtaaa ctcgccgtca acagcagcga
    20221 tgacaatatc accatggctg gcggtaatag cgctatcgac aatcagtaaa tcaccgtcac
    20281 taattccacc atcaatcata gaatcaccac ttgctttgac gaagtaagtc gcgctgggat
    20341 gctggatcaa cagttgattc agatcgatgc gctgttcaac gtaatctgct gccggtgaag
    20401 gaaagccaca ctgaacaaga tcgctaaata gcggaaaagt cacaatttcg cggagatccg
    20461 caggcttgat aaacaacata ataatctgcc tgaagttata ctgtttttat atacagtagt
    20521 ctgttcttgc cagcagatca atactgattc aggctatcaa tatttgtcgc tgcatagact
    20581 gctgattttt cgttctctta tcctgtgctc acgtggcctt ctggcgacga cgctcatcca
    20641 gcagaaatga aaaatatcgc ccggctaaaa aatagaatag aagcattcgc ccagaaaggc
    20701 attctcatta aggtactgga tgacggcatc aagaaactgt attctgttgc agcgggcgta
    20761 gttgaaggga aactgattcc agaattgaag aacaagttaa aatctgtgca gagtttcttt
    20821 accaccctgt ctaacacggt taaacaagcg aataaagata tcgatgccgc caaattgaaa
    20881 ttaaccaccg aaatagccgc catcggggag ataaaaacgg aaactgaaac aaccagattc
    20941 tatgttgatt atgatgattt aatgctttct ttgctaaaag aagcggccaa aaaaatgatt
    21001 aacacctgta atgagtatca gaaaagacac ggtaaaaaga cactctttga ggtacctgaa
    21061 gtctgata
//
LOCUS       DAZGAP010000088        21024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGAP010000088 DAZGAP010000000
VERSION     DAZGAP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.088"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..373)
                     /gene="emrE"
                     /locus_tag="ACHLAM_002781"
     CDS             complement(41..373)
                     /gene="emrE"
                     /locus_tag="ACHLAM_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO7854284.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(441..743)
                     /locus_tag="ACHLAM_002782"
     CDS             complement(441..743)
                     /locus_tag="ACHLAM_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO7854285.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(740..1441)
                     /locus_tag="ACHLAM_002783"
     CDS             complement(740..1441)
                     /locus_tag="ACHLAM_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7854286.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1438..2457)
                     /locus_tag="ACHLAM_002784"
     CDS             complement(1438..2457)
                     /locus_tag="ACHLAM_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7854287.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2454..2993)
                     /locus_tag="ACHLAM_002785"
     CDS             complement(2454..2993)
                     /locus_tag="ACHLAM_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO7854288.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3063..3293)
                     /locus_tag="ACHLAM_002786"
     CDS             complement(3063..3293)
                     /locus_tag="ACHLAM_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7854289.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3398..4087
                     /locus_tag="ACHLAM_002787"
     CDS             3398..4087
                     /locus_tag="ACHLAM_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7854290.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4683..4889
                     /locus_tag="ACHLAM_002788"
     CDS             4683..4889
                     /locus_tag="ACHLAM_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO7854291.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4965..5261
                     /locus_tag="ACHLAM_002789"
     CDS             4965..5261
                     /locus_tag="ACHLAM_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7854292.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            5267..6052
                     /gene="bet"
                     /locus_tag="ACHLAM_002790"
     CDS             5267..6052
                     /gene="bet"
                     /locus_tag="ACHLAM_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7854293.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            6049..6729
                     /locus_tag="ACHLAM_002791"
     CDS             6049..6729
                     /locus_tag="ACHLAM_002791"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7854294.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6726..6908
                     /locus_tag="ACHLAM_002792"
     CDS             6726..6908
                     /locus_tag="ACHLAM_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO7854295.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            6881..7072
                     /locus_tag="ACHLAM_002793"
     CDS             6881..7072
                     /locus_tag="ACHLAM_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO7854296.1"
                     /translation="MHKASPVELRTSIEVAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            7149..7364
                     /locus_tag="ACHLAM_002794"
     CDS             7149..7364
                     /locus_tag="ACHLAM_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7854297.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7463..7672
                     /locus_tag="ACHLAM_002795"
     CDS             7463..7672
                     /locus_tag="ACHLAM_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7854298.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            7729..8007
                     /locus_tag="ACHLAM_002796"
     CDS             7729..8007
                     /locus_tag="ACHLAM_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7854299.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            7979..8044
                     /locus_tag="ACHLAM_002797"
                     /pseudo
     CDS             7979..8044
                     /locus_tag="ACHLAM_002797"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8206..9369
                     /gene="intD"
                     /locus_tag="ACHLAM_002798"
     CDS             8206..9369
                     /gene="intD"
                     /locus_tag="ACHLAM_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO7854300.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(9385..9461)
                     /locus_tag="ACHLAM_002799"
     tRNA            complement(9385..9461)
                     /locus_tag="ACHLAM_002799"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9425..9427),aa:Arg,seq:tct)
     gene            9732..10598
                     /gene="folD"
                     /locus_tag="ACHLAM_002800"
     CDS             9732..10598
                     /gene="folD"
                     /locus_tag="ACHLAM_002800"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO7854301.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPHGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            10600..10812
                     /gene="ybcJ"
                     /locus_tag="ACHLAM_002801"
     CDS             10600..10812
                     /gene="ybcJ"
                     /locus_tag="ACHLAM_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO7854302.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVCFAGHSVQVVA"
     gene            10920..11441
                     /gene="ybcI"
                     /locus_tag="ACHLAM_002802"
     CDS             10920..11441
                     /gene="ybcI"
                     /locus_tag="ACHLAM_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7854303.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHKVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(11477..12862)
                     /gene="cysS"
                     /locus_tag="ACHLAM_002803"
     CDS             complement(11477..12862)
                     /gene="cysS"
                     /locus_tag="ACHLAM_002803"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO7854304.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            13036..13530
                     /gene="ppiB"
                     /locus_tag="ACHLAM_002804"
     CDS             13036..13530
                     /gene="ppiB"
                     /locus_tag="ACHLAM_002804"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO7854305.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            13533..14255
                     /gene="lpxH"
                     /locus_tag="ACHLAM_002805"
     CDS             13533..14255
                     /gene="lpxH"
                     /locus_tag="ACHLAM_002805"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO7854306.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            14373..14882
                     /gene="purE"
                     /locus_tag="ACHLAM_002806"
     CDS             14373..14882
                     /gene="purE"
                     /locus_tag="ACHLAM_002806"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO7854307.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            14879..15946
                     /gene="purK"
                     /locus_tag="ACHLAM_002807"
     CDS             14879..15946
                     /gene="purK"
                     /locus_tag="ACHLAM_002807"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO7854308.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPVQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(16279..17172)
                     /gene="ybcF"
                     /locus_tag="ACHLAM_002808"
     CDS             complement(16279..17172)
                     /gene="ybcF"
                     /locus_tag="ACHLAM_002808"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7854309.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTKDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(17169..17984)
                     /gene="ylbF"
                     /locus_tag="ACHLAM_002809"
     CDS             complement(17169..17984)
                     /gene="ylbF"
                     /locus_tag="ACHLAM_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7854310.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVMAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFIHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKS"
     gene            complement(17995..19254)
                     /gene="ylbE"
                     /locus_tag="ACHLAM_002810"
     CDS             complement(17995..19254)
                     /gene="ylbE"
                     /locus_tag="ACHLAM_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7854311.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(19264..20931)
                     /gene="fdrA"
                     /locus_tag="ACHLAM_002811"
     CDS             complement(19264..20931)
                     /gene="fdrA"
                     /locus_tag="ACHLAM_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7854312.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSASSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQPHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLVRLLERLQ"
BASE COUNT         5127 a         5601 c         5345 g         4951 t
ORIGIN      
        1 taagagcctc catattttag tcgtttagaa acaaattatt ttaatgtggt gtgcttcgtg
       61 acaataaatt aataaccaac acaccggcac aaatcaacat catgcctata atggctggca
      121 ggtccagccg ttggccgaaa aatccccatg acagtaagct aatcaggaca ataccgactc
      181 ctgaccagat agcataagca atccctgtag gaatataagc cagcgtctga gctaataacc
      241 agaatgatgc acaataacaa ataattgtac caacagatgg ccataaccgt gtaaaacctt
      301 ctgaaaactt cattaaggtt gtaccaatga cctctgcaag tattgcacca ccaagataaa
      361 tataagggtt catagcatat tctttcctgt tcaaactgga gagaattgta ctacagtttg
      421 aactcaactc acctgtttca tcattgtgtt cccattgatg ttcttttata taccctcagt
      481 acccgtttca tcgcggcact ctggcgacac tccttaaaaa tcagattcgt gctcaccttt
      541 ccttcccgtt cttctctggt agcgaaccgg taatacaccg ttcgccagac cttaccatca
      601 acgaccagga ttcctgcccg cgccatttta gccgcagcct gatttatgct ggttacggtt
      661 gcgcctgtta ccgcggcaac gtcctgtgca cagaagctct tatgcgtccc caggtaatga
      721 ataattgcct ctttgcccgt catacacttg ctcctttcag tccgaactta gctttgattt
      781 ctgcgatctt cgccagagcc tgtgcacgat ttagaggtct accgcccatg acaggaagtt
      841 gttttactgg ttcaggtatc gcctcaccac ggttaattcg cgcggtcata caggacagtt
      901 catcggcagc cttgcgccgt aattccgcgt cagtcaacgc attggcccgc atgttctgat
      961 acaggttggt aaccagccag tagtgcgcgt ttgatttcca cggataagac tctgcatccg
     1021 gatacagacc acgcttccgg caatactcgt aaaccatatc aaccagctcg ctgacgtttg
     1081 gcagcccggc ggtaacggat gcttcttccc ggcaccatgc aacaaactgc ccgggtgatg
     1141 gcagaaatgg tctattctgc cgacgggcaa cacgcattcc ggcgctcacc tgttccatcg
     1201 tggtgatccc gttttcccgg aaagccagaa cccactggcg gcggatttcg ttcagttcgt
     1261 tctggtcacg attagccagg ctcgccggga aagttgccag taactggctg aatacaccgt
     1321 tgattatctg cgctacctgc tgtacctgcg gcttttcgtc gtactgttcc ggcatgttgt
     1381 tggcgatccg acgcatctgc tcacggtcaa agttaatcat ctgtgcggcg atgtttttca
     1441 tagatccacc ccgtaaatcc agtctgtgtt tgtcaggtcg agttttggtt tgctagctgt
     1501 cacgcctgcc tgttgcttgt tacggttgat ttcgagttgg gtccacttgt cgcggagttt
     1561 ggccggactt agcacgttac cggaccagaa gttgtcctgg catgcccagc ggaacagcac
     1621 gcacatgtcg cggtggttac gtccgtcacg ttcacgcatc aggcggatat cgttagccca
     1681 ccctgcaaaa ttcggttttc tggctgatgg cgcgatggtc ttcaccatgt caaacatcca
     1741 ctctgcggcg gtcaggtctt ctgctgtccc ccacttgctg ccgctctgaa ttgcagcatc
     1801 cggtttcacc acaggaagat cgttttctgg ttggtcagag gattcgccag aattctcgga
     1861 cgaaaaaggt tttatattgt cttttgttag tttgtctttt gtgtttacct gattcgggta
     1921 aacgccttta cctgatttgg gtaaactttt tttacctgat tcaggtaaat ttacctcttt
     1981 caggtaaact ttatttttct tacctgattc gggtaatgtt gaccattcac tgaccacatt
     2041 attaatgccg gtattccgcc cgctctgaat aaaaatccca cgctttacca gaacactttt
     2101 tgcagcagaa cacttgtgcg gcaatatccc ggttaattcg gaaagttgct cgttgctaac
     2161 ccaatccagt tttttattaa agccatatgt tttgcgcatg acagccagaa agaccagaag
     2221 ctggtgctgt gttaatccgg ccagcatcac agcttccagc aactcatttg caatgcgcgt
     2281 ataaccatca tcgagatctg ccacgcgcgg ctccttttgt gccgcatccg gcactggaaa
     2341 attgaatatc tcagcagtgt ttgccataat tcctcccgca atgagtgtgt tacgatttgc
     2401 acctgaaagt cggttctgtt ccagcagacc ggctttcgcc atttctgaac ctgtcatatc
     2461 gcccccagca tggtagtaac catcgccatc aatggaccag ccagatctgg gtccacacga
     2521 aacatcgaca caataccttc actaatttcc ttcagtttct ggtggcgtgg tgcgttgaga
     2581 atgacagcct gttttgcctc actgagttcc ttttccattt cagccaacct agccatgaag
     2641 ctatcctgct caaccaggta accgcgatat tccagcggta gtaccgccag aattgccggg
     2701 gtcagttcac gcacgttatt tcggtatttt tcagaatcga atttgttatc gaggaagcgg
     2761 aacagcttct ggcgtgcacg gctgacatca tcagggaaat cgatggtgcc gccgccctgc
     2821 tcccgatact cattcacaat gagtgtggca acgacatcct gattatctac agccgaccag
     2881 gcgcggacgg catcacggat tttttcgtgg cctggcacct gttttgtttg agaacgattt
     2941 atcaccgcag tcgggctaaa tccgctagtc tgttggtatg taagtggttg cataattgac
     3001 tcctttagtt tgaattgact gttaagttga ttgcttattg ttaaagagcg tgaaatggaa
     3061 atttaagctg cgttcttttc ggtgtgtgga aacaacttcg gaagatccgg gcgaatctgg
     3121 tatgccttca ctactccacc agtagccgta acaatgctgc cgacatgttc aggggatacc
     3181 tttgctttgt tgtgaagcca cttatagacg gcctgctgtg aaacttcgca agcagcgccc
     3241 agtttctttt gtgaaccaac gatattgatc gctgttttga tagctgggtt cataacaacc
     3301 tccgtggtta atttgaatca agattaaaac tatggttgtt tttagtcaac aaccattttc
     3361 gtttgatgga ataaaacctt ggttgtacat ttggactatg aaaacaacac tctcagaaag
     3421 acttaaagaa gccagattag cgcgaggcct tacacaaaag gcgcttgggg atttggtcgg
     3481 ggttagccag gctgctattc agaaaatcga aacagggaaa gctaatcaaa caactaaaat
     3541 cgtggagatc gcgaacgctt tgggtgtgcg cgcagaatgg ttatcttctg gcgttggaaa
     3601 tatgtcagac agtacagtgc aaccaataca atcaactgtc agccattcca aatacttcaa
     3661 gattgacgtt cttgatatag aagtcagtgc tgggccggga gtcatcaacc gtgagtttgt
     3721 agaagttcta cgctcggttg agtactcgtt tgacgatgct cgtcacatgt tcgatggtag
     3781 gaaggcggaa aatatccgca tcattaacgt gcgtggtgac agcatgtcag gaacgatcga
     3841 accaggtgat ctgctgttcg ttgatatcac agttaaatct ttcgacggtg atggtatcta
     3901 tgcgtttctg tacgacgaca cagcccatgt aaagcgcctg caaatgatga aggataagct
     3961 gctggtcatc tctgataaca aaagctactc accgtgggac ccgatcgaga aagacgagat
     4021 gaaccgggtg ttcatcttcg gtaaggttat tgggagcatg ccgcagacat ataggaagca
     4081 tgggtagtac caattaaaaa ttatcaactg ggcattgtgc tcattcagta aagaactaat
     4141 tcctatcttt gctctaggta gtaatattaa gccaccgcaa taatatcttt acctaacggc
     4201 gtaagaatcc cggtcaccgt gccgggtttt cttttgccct cccctcatca cacacaccgt
     4261 taaaaaaacc accataacct cgcttcagtt atcgctatgc gattcaagtc acaaaataaa
     4321 tccatcctaa atacaaccag ttatatctaa aacaaccaat aaaacaactt ttgttgttga
     4381 cgataaaaca actatagttt taaataagtt catcgcaaca acacaacgat acggcaacta
     4441 cctgattcac cgttgcgatg accgcttaga tccgcagttt gaatttcagc aggcttcggg
     4501 gagtgcgagg ggtgaaacgg acgcgtgaac gtcggtgtga ccagctgaaa ttaactcaac
     4561 atttcatacc ttagtcgctt caacgaggcg gcttagttat gacaaccggc ggccatccac
     4621 cgcctgaata cgcgcagaag tctctatatg ttcagcagcc cagcttacgg gcaggagttt
     4681 ttatggttca tcaacattat ggaacgcaga ccgttaatcg aggtgcggtc atgccaggaa
     4741 tgctggtcaa acacaaagat ggtacctgga ctgcatcagc taatttacgc ggacggcttt
     4801 atctgcatcg cggcatcgag cgcacttata cccgtgattt gctcgtggaa gtttttctcg
     4861 acggacgcgg taacggcctg aatcactaat cccctttcct gttttcctaa tcagcctggc
     4921 atttcgcggg cgatattttc acagccattt tcaggaggtc agccatgaac gcttattaca
     4981 ttcaggatcg tcttgaggct cagagctggg cgcgtcacta ccagcagatc gcccgtgaag
     5041 agaaagaggc agaactcgca gacgacatgg aaaaaggcct gccccagcac ctgtttgaat
     5101 cgctatgcat cgatcatttg caacgccacg gggccagcaa aaaagccatt acccgtgcgt
     5161 ttgatgacga tgttgagttt caggagcgca tggcagaaca catccagtac atggttgaaa
     5221 ccattgctca ccaccaggtt gatattgatt cagaggtata aaacggatga gtacagcact
     5281 cgcaacgctg gcagggaagc tggctgaacg tgtcggcatg gattctgtcg acccacagga
     5341 actgatcacc actcttcgcc agacggcatt taaaggtgat gccagcgatg cgcagttcat
     5401 cgcattgctg atcgtcgcca accagtacgg ccttaatccg tggacgaaag aaatttacgc
     5461 cttccctgat aagcagaacg gcattgttcc ggtggtgggc gttgatggct ggtcccgcat
     5521 catcaatgaa aaccagcagt ttgatggcat ggactttgag caggacaatg aatcctgtac
     5581 atgccggatt taccgcaagg accgtaatca tccgatctgc gttaccgaat ggatggatga
     5641 atgccgccgc gaaccattca aaacccgcga aggcagagaa atcacggggc cgtggcagtc
     5701 gcatcccaaa cggatgttac ggcataaagc catgattcag tgtgcccgtc tggccttcgg
     5761 atttgctggt atctatgaca aggatgaagc cgagcgcatt gtcgaaaata ctgcatacac
     5821 tgcagaacgt cagccagaac gcgacatcac tccggttaac gatgaaacca tgcaggagat
     5881 taacactctg ctgatcgccc tggataaaac atgggatgac gacttattgc cgctctgttc
     5941 ccagatattt cgccgcgaca ttcacgcatc gtcagaactg acacaggccg aagcagtgaa
     6001 agctcttgga ttcctgaaac agaaagccac tgagcagaag gtggcagcat gataccggac
     6061 attatcctgc agcgtaccgg gatcgacgtg agagctgtcg aacaggggga tgatgcatgg
     6121 cacaaattac ggctcggcgt catcaccgct tcagaagttc acaacgtgat agcaaagccc
     6181 cgctcaggaa agaagtggcc tgacatgaaa atgtcctact tccacaccct gctggctgag
     6241 gtttgcaccg gtgtggctcc ggaagttaat gctaaggcgc tggcctgggg aaaacagtac
     6301 gagaacgacg ccagaaccct gtttgaattc acttccggcg tgaatattac tgaatccccg
     6361 atcatctatc gcgacgaaag tatgcgcacc gcctgctccc ccgatggttt atgcagtgac
     6421 ggcaacggcc ttgaactgaa atgcccgttt acctcccggg atttcatgaa attccggctc
     6481 ggtggtttcg aggcaataaa atcggcttac atggcccagg tgcagtacag catgtgggtg
     6541 acgcgaaaag atgcctggta ctttgccaac tatgacccgc gcatgaagcg tgaaggcctg
     6601 cattatgtcg tgattgagcg gaatgaaaag tacatggcga gttttgacga gatggtgccg
     6661 gagttcatcg aaaaaatgga cgaggcactg gctgaaattg gttttgtatt tggggagcaa
     6721 tggcgatgac gcatcctcac gataatatcc gggtaggcgc gatcactttc gtctactccg
     6781 ttacaaagcg aggctgggta tttcccggcc tttctgttat cagaaatcca ctgaaagcac
     6841 agcggctggc tgagaagata aataataaac gggaggcggt atgcacaaag catctcctgt
     6901 tgagttaaga acgagtattg aggtggcaca tagccttgct caaattggag tcaggtttgt
     6961 gccaatacca gtagaaacag acgaagaatt tcatacgtta gccacatccc tttcacaaaa
     7021 gctggaaatg atggtggcga aagcagaagc agatgagaga gaccaggtat gacaaccact
     7081 gaatgcattt ttctggcagc gggcttcata ttctgtgtgc ttatgcttgc cgacatgggg
     7141 cttgttcaat gacacctcag caagaaaacg cccttcgcag cattgcccgt caggctaatt
     7201 ctgaaatcaa aaaagccaga cagcattttc cggataaaaa cgtcgatgac atttgccgta
     7261 gcgtactaaa gaagcaccgc gaaacggtaa cgctgatggg attcacaccg actcatttaa
     7321 gcctggcgat cggcatgttg aacggcgtct ttaaggaacg gtaaacatga aaagcaaaat
     7381 catcagggag ctacaggctc cttttttatt attcgcattt accctcaagc gtattaacca
     7441 acaattcagg gattaatgaa agatggcaga catcattgat tcagcatcag aaatcgaaga
     7501 attacagcgc aatacagcaa taaaaatgcg tcgtctgaac taccagactg tatccgcaac
     7561 tcattgttgt gagtgtggcg atccgataga tgagcgaaga cgcctggctg ttcagggttg
     7621 tcggacttgt gcgagttgcc aggaggagat cgaacttaag aacaaacaat agggactgtg
     7681 atggcctcaa agcagcaaat ttcaacatcg tccaactgag gtgtaaaaat gttcagaatc
     7741 atttttccta acacctggta cgtcgaccac cacggcactc cctgcaaaat cctgcgttct
     7801 acccacaaca aagttcacta catccgaaaa ggcagaacat gtatcgccag catgttccgc
     7861 tttaatcatg actttgaacc tgtgaataaa gctgatgcag atcggatagc agaagagatc
     7921 gaaacggcag aacacattaa gaagttacgt gacatgcgtt caaaaagcag aggtaaccat
     7981 ggaatcatac agcctcacac tcgatgaggc ctgtcagttt cttaagatat ccagaccaac
     8041 cgccaccaac tggatacgaa caggccgcct acaggcaaca cgtaaagatc caaccaagcc
     8101 aaaatctcct tacctcacaa cacggcaagc ctgcattgcg gcgcttcagt ctccgctgca
     8161 tactgtccag gtgagcgcgg gtgatggcat aacagaggaa agaaaatgtc actcttccgc
     8221 agaaatgaaa tatggtatgc ctcgtattcg ctcccgggcg agaaacgaat taaggaatct
     8281 cttggcacaa aggacaagcg gcaagctcag gagttgcacg acaagcgaaa agcagaactc
     8341 tggcgagtag aaaagctagg ggatttacct gatgtcactt ttgaagaggc ctgcctaaga
     8401 tggcttgagg aaaaagctga taaaaaatct ctcgattcag ataaaagccg gattgagttc
     8461 tggcttgaac attttgaggg tataaggctt aaagatatct cggaggcaaa gatttactct
     8521 gctgtaagca gaatgcataa cagaaagacg aaagaaatat ggaaacagaa agttcaggcc
     8581 gccatcagga aaggtaaaga accgcctgtt tatgaaccaa agccagtatc aactcagaca
     8641 aaggcaaagc atcttgccat gataaaggcc attctccgtg ctgcagaacg cgactggaag
     8701 tggctggaaa aagcgcctgt catcaagata ccagcggtca gaaacaagcg agtcagatgg
     8761 ctggaaaagg aggaagcaaa acgccttatt gatgagtgcc ccgaaccact gaaatctgtc
     8821 gtcaagtttg cgctggcaac tggtctgaga aagtcgaaca tcataaatct ggaatggcaa
     8881 caaatcgaca tgcagcgacg agttgcctgg gtgaatccag aagagagcaa atcaaaccgc
     8941 gccattggtg tggcgctgaa cgataccgcc tgtaaagtgt tgcgtgatca aataggcaag
     9001 catcacaaat gggtgtttgt acataccaag gcggctaagc gagcagatgg aacatcaacg
     9061 cctgcggtca ggaagatgcg catcgacagc aagacatcat ggctatcagc ttgtcgtcgt
     9121 gcaggaattg aagatttccg tttccatgac ctcagacaca cctgggcaag ctggctgatt
     9181 cagtcaggcg tcccattatc agtgcttcag gaaatgggcg gatgggagtc catagaaatg
     9241 gttcgtaggt atgctcacct tgcgcctaat catttgacag agcatgcgag gaaaatagac
     9301 gacatttttg gtgataatgt cccaaatatg tcccactctg gaattatgga ggatataaag
     9361 aaggcgtaac tgattgaatt gtaatggcgc gccctgcagg attcgaacct gcggcccacg
     9421 acttagaagg tcgttgctct atccaactga gctaagggcg cattgatacc acggtgtgct
     9481 gtaatcgcgt gaattatacg gtcaaccctt gctgagtcaa tggcttttga accagttgct
     9541 gaacaagtgt acgaccgcat cagatgtagc tcactatggc ggcaaaccaa cgacggttat
     9601 catgataggt gtgaatcagc caggtacaga aggttttact tttcagaaaa cgaaaactga
     9661 cagcgcgcca cgcttctgac aaaatagacg catccccttc gacctacgta acagatggaa
     9721 tcctctctct gatggcagca aagattattg acggtaaaac gattgcgcag caggtgcgct
     9781 ctgaagttgc tcaaaaagtt caggcgcgta ttgcagccgg actgcgggca ccaggactgg
     9841 ccgttgtgct ggtgggtagt aaccctgcat cgcaaattta tgtcgcaagc aaacgcaagg
     9901 cttgtgaaga agtcgggttc gtctcccgct cttatgacct cccggaaacc accagcgaag
     9961 cggagctgct ggagcttatc gatgcgctga atgccgacaa caccatcgat ggcattctgg
    10021 ttcaactgcc gttaccggcg ggtattgata acgtcaaagt gctggaacgt atacacccgg
    10081 acaaagacgt ggacggtttc catccttata acgtcggtcg cctgtgccag cgcgcgccgc
    10141 gtctgcgtcc ctgcaccccg cacggtattg tcacgctgct tgagcgttac aacattgata
    10201 ccttcggcct caacgccgtg gtgattggcg catcgaatat cgttggccgc ccgatgagca
    10261 tggaactgct actggcaggt tgcaccacca ccgtgactca ccgtttcact aaaaatctgc
    10321 gtcaccacgt agaaaatgcc gatctactga tcgttgccgt cggcaagccg ggctttattc
    10381 ctggtgactg gatcaaagaa ggcgcaattg tgattgatgt cggcatcaac cgtctggaaa
    10441 atggcaaagt tgtgggcgac gtcgtgtttg aagacgcggc taaacgcgcc tcatacatta
    10501 cgcctgttcc gggcggcgtt ggcccgatga cggttgccac gctgattgaa aacacgctac
    10561 aggcgtgcgt tgaatatcat gatccacagg gtgagtaaca tggcgacatt ttctttaggt
    10621 aaacatccgc acgttgagct gtgcgacttg ctgaaactgg aaggctggag cgaaagcggc
    10681 gcgcaggcga aaatcgcgat tgccgaaggc caggtgaaag tcgacggtgc ggttgaaacg
    10741 cgcaaacgct gcaaaatcgt cgccgggcag acagtgtgtt ttgcaggtca cagcgtacag
    10801 gttgttgcct gatccctttc ccggcctgta acaacaggcc ggttctcttt tcagactttt
    10861 gccctctacg ccccgttcat ccatgctgat agcatcgaca ttttgccgaa gcgaccatca
    10921 tgccaaccgt tattacgcac gccgccgttc ccctttgcat tggtttaggg ttgggttcga
    10981 aagtcatccc tccgcgtctg ttatttgccg gaatcgtcct ggcgatgctg cccgatgccg
    11041 acgtgttgtc gtttaaattt ggcgttgctt acggcaatgt ttttggtcat cgcgggttta
    11101 cccattcgct ggtgtttgcg tttgttgtcc cgctgttatg tgtgctgatt gggcgtcgat
    11161 ggttcagggc tgggctgatt cgctgctggc tgtttttaac cgtctcgttg ctatcgcaca
    11221 gcttgctgga ttcggtaacc actggcggta aaggggttgg ctggctgtgg ccgtggtcag
    11281 atgaacgctt tttcgctccc tggcaggtga ttaaagtcgc gccgtttgca ttgtctcgtt
    11341 acaccacgcc gtacgggcat aaggtgatta tttctgaatt gatgtgggtg tggttgccgg
    11401 ggatgctact gatgggaatg ttgtggtggc gcagacgata accggatgcg aaaactcgca
    11461 tccggcaata gcgcaattac ttacgacgcc aggtggtccc ttgcgggcca tcttccagca
    11521 cgatccccat ctcgttaaga cggtcacgcg ccgcatctgc cgccgcccag tctttcgctt
    11581 tacgggcatc cagacgctgt tgaattaacg cttcaatctc agccacttca ctgtcgtctg
    11641 cctgcgcgcc gctttgcagg aacgcttccg gttcttgctc cagcaggccc aatacagcgg
    11701 aaagtttacg caggtgagac gccattgcat tcgccgctgc catatcttct gctttcagac
    11761 ggttcacttc acgcgccata tcaaacagta cggaataggc ttccggggtg ttgaaatcgt
    11821 cgttcatcgc ctcaataaag cgcgcttcaa acgcttcgcc accggcagga gcaacggttt
    11881 tgtctgtgcc gcgcaaggca gtatagagac gttccagcgc cgcacgcgcc tgcttcaggt
    11941 tctcttcgct gtagttcaac tgactgcgat agtgacccga catcaggaag taacgcacag
    12001 tttccgcatc gtagtatttc agcacatcac gcacggtgaa gaagttaccc agcgatttag
    12061 acatcttctc gcggtcaacc atcaccatcc ccgagtgcat ccagtagttc acatactgac
    12121 catcatgggc acaggtggac tgggcaattt cgttctcgtg gtgcgggaac atcaggtcag
    12181 aaccgccgcc gtggatatca aagtggttac ccaactgttt gcagttcatt gccgaacact
    12241 caatgtgcca gccaggacgg cccgcgcccc acggagacgg ccagctcggt tcgccctctt
    12301 tcgacatctt ccacagaacg aaatccatcg ggttgcgttt gtcgtcgacc acgtcaacgc
    12361 gcgcgcctgc ctgcagctga tcgagatcct gacgcgacag cacgccataa gttggatcgg
    12421 tcgggacgtc gaacatcacg tcgccgttgt ccgccacata agcgtgacct ttggcgatca
    12481 gttgttcagt gagttcaata atttcggcga tatggtgcgt cgcgcgcggc tccatatccg
    12541 ggcgcagaat gttcaaagca tcaaaatctt tgtgcatttc ggcgatcatg cgatccacca
    12601 gcgccacaaa gctttcgcca ttttcattgg cgcgtttgat gattttgtcg tcgatatcag
    12661 taatgttgcg cacatacttc agcttatagc cgaggaaacg cagatagcgc gcaaccacgt
    12721 caaaggcaac aaaggtacgc ccgtgaccga tatgacagag atcgtaaacg gtgattccac
    12781 acacgtacat gccgacttcc ccggcgtgaa taggcttaaa ttcctctttt tggcgtgtca
    12841 gagtattgaa gatttttagc atcgaagatt ccgtttagac atgtgtgggt aattgagttg
    12901 cgtataatac ccatatttcc cgcgcgaatc agcatacatt gcgtgatgat ccgatcgtgc
    12961 ggttatgcta taacaccacc ctatatatga cccgaaccgg gttgaagcac caatcaaacg
    13021 gaacaggatg caaaaatggt tactttccac accaatcacg gcgatattgt catcaaaact
    13081 tttgacgata aagcacctga aacagttaaa aacttcctgg actactgccg cgaaggtttt
    13141 tacaacaaca ccattttcca ccgtgtaatc aacggcttta tgattcaggg cggcggtttt
    13201 gaaccgggca tgaagcaaaa aaccaccaaa gacccgatca aaaacgaagc taacaacggt
    13261 ctgaaaaata cccgtggtac actggcaatg gcccgtactc aggcaccgca ctctgcaact
    13321 gcacagttct tcatcaacgt ggttgataac gacttcctga acttctctgg cgaaagcctg
    13381 caaggttggg gttactgcgt gtttgctgaa gtggttgaag gcatggacgt ggtagacaaa
    13441 atcaaaggtg ttgcaaccgg tcgtagcggt atgcaccagg acgtgccaaa agaagacgtt
    13501 atcattgaaa gcgtgaccgt tagcgagtaa tcgtggcgac actctttatt gcagatcttc
    13561 atctctgcgc ggaagaaccg gcgatcaccg ccggttttct gcgtttttta gcgggggaag
    13621 cccgcaaggc cgacgcgctg tatattcttg gcgatctgtt tgaagcatgg attggcgacg
    13681 acgatcccaa cccactccat cgccagatgg cggcagcgat caaagcggtg tccgattccg
    13741 gcgttccctg ttatttcatt catggcaacc gtgattttct gctcggcaaa cgctttgccc
    13801 gtgaaagcgg catgacgtta ttgccggaag aaaaggtgct cgaactttat ggtcgccggg
    13861 tgttgattat gcatggcgac acgctgtgca ccgatgacgc cggttatcag gcttttcgcg
    13921 ccaaagtcca caaaccctgg ctgcaaacgc tattcctcgc cctgccgttg tttgtgcgca
    13981 aacgcattgc cgcgcgaatg cgcgcgaaca gcaaagaagc caacagcagt aaatcgctgg
    14041 cgatcatgga cgttaaccaa aacgcggtgg tcagtgcgat ggaaaaacat caggtgcaat
    14101 ggctgattca cgggcatacc catcgcccgg cagtacatga acttatcgcc aatcagcaac
    14161 ctgctttccg cgtcgtgctg ggtgcctggc atactgaagg ttcgatggtg aaagtcacgg
    14221 cggatgacgt tgagttgatc cattttccat tttaaaaaac gcgcaacttt gctgatttca
    14281 taaccgcgca accgttttcc ttgctctctt tccgtgctat tctctgtgcc ctctaaagcc
    14341 gagagttgtg caccacagga gttttaagac gcatgtcttc ccgcaataat ccggcgcgtg
    14401 tcgccatcgt gatggggtcc aaaagcgact gggctaccat gcagttcgcc gccgaaatct
    14461 tcgaaatcct gaatgtccca caccacgttg aagtggtttc tgctcaccgc acccccgata
    14521 aactgttcag cttcgccgaa agtgccgaag agaacggtta tcaggtgatt attgcgggcg
    14581 caggcggcgc agcgcacctg ccaggcatga ttgccgccaa aacgctggtg ccagtgctgg
    14641 gcgtgccagt acagagcgcc gcactgagcg gtgtcgatag cctctactcc atcgtacaaa
    14701 tgccgcgcgg cattccggtg ggtacgctgg cgattggtaa agctggcgcg gcaaacgcgg
    14761 cgttactggc agcacaaatt cttgcgactc atgataaaga actgcaccag cgtctgaatg
    14821 actggcgcaa agcccagacc gacgaagtgc tggaaaaccc ggacccgcga ggtgcggcat
    14881 gaaacaggtt tgcgtcctcg gtaacgggca gttaggccgt atgctgcgtc aggcaggcga
    14941 accgttaggc attgctgtct ggccggtcgg gctggacgct gaaccggcgg cggtgcctgt
    15001 tcaacaaagt gtgattaccg ctgagatcga acgctggccg gaaaccgcat taacccgcga
    15061 gctggcgcgc catccggcct ttgtgaaccg cgatgtgttc ccgattattg ccgaccgtct
    15121 gactcagaag cagcttttcg ataagctcca cctgccgacc gcgccgtggc agttacttgc
    15181 cgatcgcagc gagtggcctg cggtgtttga tcgtttaggt gaactggcga ttgttaagcg
    15241 tcgcactggt ggctatgacg gtcgcggtca atggcgttta cgcgcaaatg aaaccgaaca
    15301 gttaccggca gagtgttacg gcgaatgtat tgtcgagcag ggcattaact tctctggtga
    15361 agtgtcgctg gttggcgcgc gcggttttga tggcagcacc gtgttttatc cgctgacgca
    15421 taacctgcat caggacggta ttttgcgcac cagcgtcgct ttcccgcagg ccaatgcgca
    15481 gcagcaagcg caagccgaag agatgctgtc ggcgattatg caggagctgg gctatgtagg
    15541 cgtgatggca atggagtgtt ttgtcacccc gcaaggtctg ctgatcaacg aactggctcc
    15601 gcgtgtgcat aacagcggtc actggacaca aaacggtgcc agcatcagcc agtttgagct
    15661 gcatctgcgg gcgattaccg atctgccgtt accgcaaccg gtagtgaata gtccgtcggt
    15721 gatgatcaac ctgattggta gcgatgtgaa ttatgactgg ctgaaactgc cgctggtgca
    15781 tctgcactgg tacgacaaag aagtccgtcc ggggcgtaaa gtggggcatc tgaatttgac
    15841 cgacagcgac acatcgcgtc tgaccgcgac gctggaagcc ttaatcccgc tgctgccgcc
    15901 agagtatgcc agcggcgtga tgtgggcgca gagcaagttc agttaattga tgctcatttt
    15961 cttgccggat gcggcgtgaa cgccttatcc ggcccaccgc tccaacaccg actgtaggcc
    16021 tgataagacg cgccagcatc gcatcaggca tggtgtgccg attgcaggat gcggcgtgaa
    16081 cgccttatcc ggcctaccgt tccaacaccg actgtaggcc tgataagatg cgccagcatc
    16141 gcatcaggca tggtgtgccg attgccggat gcggcgtgaa cgccttatcc ggcctaccgt
    16201 tccgacaccg cctgtagacc tgataagacg cgccagcatc gcatcaggct agatgtgcca
    16261 attgcctggt gcccatcact acagcgaaat acaagtcccc gcttcgcccg ccagcgtctc
    16321 ttcaatccgc gataacgccc caatccacgc gggtttgcca cggcttctga catagccact
    16381 caccgcagtc accttcggcc ccatcgaacc atcggctttg gcaaatggcg ctaactcatc
    16441 cggtgtggca tggcgaatgg cacgttgctg cggcgttccc cagttttcat ataccgcatc
    16501 agcatcggtg aggatcacca gtccatcggc attaatctgc tcggcgagca acgcagcggc
    16561 gagatcttta tcaattaccg cttcactccc tgccccgtct ttcgtcacag gcacaccgcc
    16621 gccgccactg caaatcacca catgcccctc tttgagcaac aactcgatgg cttcgctgtc
    16681 gagaatttta cgcggttgcg gagacgccac cacccggcgt aaatatttac catcacgttt
    16741 catctgccag ccataagccg cttccagtgc ctcttgttct tctggctgat aaaccggacc
    16801 gataaacttc tctggctgca aaaacgccgg atcatcaggc gaaacttcaa tgcgcgtcag
    16861 cactgtcgtg acaggcggca tctgcggctg tgcgctcaag ctctgcgcca gcatatagcc
    16921 aatcatcccc tggctttccg ccaccagcac atccagcgga tacggttcta cctctttcca
    16981 cgccagattc tgtaacgcca gcagccccac ctgcggcccg ttgccgtgaa caatcgccag
    17041 ccgataagaa cgggccaggc gcgccagtgc gggtacagca ctggcaatgt tgcgatattg
    17101 attttctgcc gtcagcgcct caccgcgctg gagtaaggcg ttgcccccaa gagccacaac
    17161 cagtgttttc atgattttcc ttgtaataat tgttggccaa gccagaaccc cagcagcgta
    17221 tctgcccctg acgtatgccc cagcgccagc agcgaatcaa tcgcaacagc ggtacgtttc
    17281 gggcaactca gagcatgaat aaagtgcagg agtggcgagg cgaagtatcc ttgcgcggca
    17341 taacgtaaat aactgacgct caccgctgtg gtaacgagtt gaagattgtc ggaacaggca
    17401 aaaaacgggc gaccggagcg cgcatctaaa gcgccataat accaggccgc cagcagcatt
    17461 ccgctcagcg tgtcatcatg acttggcgtt aatcctgggc ctttacccag ccagtgccgc
    17521 cagtcggtct taacgccatt gagcgcggcc tgaaaacagt gacgaaactg gcgtaattca
    17581 gcaggaagcg gatcgcctgc cgccaacgcc agtggcccga aaagcccggt ttcctccgcg
    17641 cgttgcatcc atgccgctgc aagcggttga ggatgcgcag gcggcgtaat acgcagcaaa
    17701 caataacgct gtggctgttt aaccgtgaaa cgtccgaggc gaatcccttg agccataacc
    17761 tgtggtcgtt cattgccgca taatccaccg cataacgcat cgaattgcgc gcggcgaagc
    17821 acccatcctc cggggccgaa accactaccc tgacgatgca gcgttaacag ttcgccgctt
    17881 tccgtcatca ggttaatcgc ccgccgccac actcccgcta accgccagga ctggcgataa
    17941 ttcggtgcgc tactactggc aagcagagga tggatgatcg tcattgtgcg ttcctcaacc
    18001 aatccccatg ctttcagcca gtgcttccag cgcctgctca aagcacgcca gcggtgcccg
    18061 cacggtgcct gcgccaatct gcccgatccc cgcctcttta tgggcgattc cggtattgat
    18121 gagcggcgta atgccggttt ctaccacgcg acgaatatcc agccccaggc acgcgccctg
    18181 gaaatcccag ccggggatct gcaactgcat attgcgttca aggaagattt ccgccatctc
    18241 ttcagatacc gctctggccg cttccatgcc acccgcaccg acaaagcgcg ttacgccagg
    18301 cgctgcgatc atcgccgcac cgccaatacc aaaggtttcg gtaattgcgc tatcgcccat
    18361 atccgggttc gcctgctcct gcgagaagcc ggtgaaaaac agcccttggg gagtgtttac
    18421 aggcgcagta aaccagcgtt cgcccagccc gcttacccga atcccgaaca tattgccgtt
    18481 gcgggtcata gccgtgacga tgctgcctgc gcggatcatc gcaccagcat ccatcgccgc
    18541 cttgcagtaa gccatcgcga ggttaaggaa gaactgatcg gtcacgctga ggaaatccat
    18601 cacttccgcg atgtgctgtt tatcatgatc gaggcgagca atgtgtgggg ccagcgcacg
    18661 cattaacagt gctgaggaag caatattgcg ttgatggaac tcatcgccca tggtaatgcc
    18721 ctgcgccatc atcgcggtga gatcgatacc gcgctccatg cgccccagcg ccgcgcttaa
    18781 tactggcatt aacacatcgc gcatccagcg atggcgagtc agaacatctt cgccgtaagc
    18841 gccaaaacgc atcactttgc cgataccttc gttgaggttg cagtacgccc ggttgccgtc
    18901 ggtcacgttc tcgaccacca gcatcggcat actggcagaa gtaatgccgc ccattggccc
    18961 gacggcattc acatggtgac aaggaatgaa gttcacttcc ccctgatcca gcattgccag
    19021 cgcctgcgct tcatctttcg cccagccttc aaacagacat gcgcccacac atgccccttt
    19081 catgggtccg gtcatctcct gccagcgcat tggcggcccg gcgtgaagca gtgttttgcc
    19141 ctcgtttagt tcgctgataa gtgaagaagc cggttgcaca tccagccagt gtggacgagc
    19201 gcgacgaatt tgttcgataa ccgcagcatt ggcttgcgcc actgatgtaa acatgggaac
    19261 cccttattgc aaacgttcta ataaacgaac cagtttttta ttgccaccgg cgactggcga
    19321 ccattggtaa tgcacaaccg gtttgctggc gctttgtagc tccagcgcaa agctgcgtaa
    19381 tccgatgtta atcacggcga cgttttccag taatgacggt gtatgtggct gtgtggcagg
    19441 cgagagcgga tgaattaacg ccgccgccag caaggtggct tccggcagcg aactcacgac
    19501 cgcaatcccc gcatcttcca gcgtggcgat ttgctgcgag cggcattgcg ggtcacgttc
    19561 agtgcctgtc accgtggcaa tggcgtacag cggttggctg tcagaacgcg cggcacaggc
    19621 tttttgccag gcgctcacca gcgaggcggc aggatcggcg gtcgcaccga agccaatcac
    19681 gacatcaagc agcaacacgc gcacttgcgg tttagcgccg agatcggcaa ttaacaggtt
    19741 gcgtaaggtc ggatcgatca tcggatgggg acgcccgacg gtgtaaaaat catcgcccag
    19801 gtcgaggatc tgatgtccgt cggcgtccag catcatgcca tgttgatggg tatcgtcggc
    19861 ttccacgcca aggtgtccgg caagtaatcc cgccgcttca gcagccagcg taccgccggt
    19921 atacaagccg caaataaatc cgctgctgac aggcgctatt gcgttacgtc gcgcagtgac
    19981 gcgtgaaagc agacaagcca ggcgtgcggc ctcatccagc gaggaggcaa accagacatt
    20041 ctcgtcgcgg gccaccgccg gggtataacc taaaaacagc gccaccgtcg gtttgccagt
    20101 tgctttcatg gcattaacaa ttttcagacg caccgcttcg gcaggtggtt ttgaaacaaa
    20161 tgccagcact tcgcttttct cgtctgcact gagcatttcc agcgccgtca gcgcactgat
    20221 gccgcccact tcacggctga ggtcgcgccc gccaaggcca atcgcgtgag taattccctc
    20281 ccctgccagc gcaatctgtg agcacagctc ctgaatcccg gtaccggaag cgccaatgac
    20341 gccaatgttg ccttccggta tcacgttagc aaaagccagc ggcgtgccgg caatcatcga
    20401 cgtaccgcag tccggcccca tcaccagcaa gcctttttcc cgcgcgcggg ttttaagttg
    20461 gatttcatct tccagcgtga cgttatcgga gaacatcatc acgttgaggt tgcgatccag
    20521 cgcctggttc gccagctccg ccgcatactc accagccact gaaatcagcg ccagattggc
    20581 atcgggtaat ttctgacagg cactgtccca gcgacgcacc tgcgtcaacg cctggctgct
    20641 ggctgacccc tgcgccagtt gttttagcgc ctcttcaagc tgctgcataa tcgcctgcgc
    20701 gatccccgca tccgccgctt cgctacgaat tgccacgcaa atatcgttcg gcgtggcgtt
    20761 attaaaatcg tcatgccaga aacctgtggt atctaataac gctttattgg cgggcgtacc
    20821 catcattacg gaaacatcat caacattttc tgattcgctg agttttcgtg aaataatcat
    20881 taaactgacc gaatcctgaa aacacccttt tttaataaag gcgtggatca taacaactcc
    20941 ttaaaaattg cacccatggc aattgcacga gcagtgttaa tgtggcgatt acggtatcga
    21001 tgacaacgag taaataaggt gatg
//
LOCUS       DAZGAP010000089        20905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGAP010000089 DAZGAP010000000
VERSION     DAZGAP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.089"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..406)
                     /gene="yiiR"
                     /locus_tag="ACHLAM_002812"
     CDS             complement(<1..406)
                     /gene="yiiR"
                     /locus_tag="ACHLAM_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7854313.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQD"
     gene            507..1106
                     /gene="yiiQ"
                     /locus_tag="ACHLAM_002813"
     CDS             507..1106
                     /gene="yiiQ"
                     /locus_tag="ACHLAM_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO7854314.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1214..1981
                     /gene="tpiA"
                     /locus_tag="ACHLAM_002814"
     CDS             1214..1981
                     /gene="tpiA"
                     /locus_tag="ACHLAM_002814"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO7854315.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2036..2791)
                     /gene="cdh"
                     /locus_tag="ACHLAM_002815"
     CDS             complement(2036..2791)
                     /gene="cdh"
                     /locus_tag="ACHLAM_002815"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO7854316.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2897..3886)
                     /gene="sbp"
                     /locus_tag="ACHLAM_002816"
     CDS             complement(2897..3886)
                     /gene="sbp"
                     /locus_tag="ACHLAM_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO7854317.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4206..5168)
                     /gene="pfkA"
                     /locus_tag="ACHLAM_002817"
     CDS             complement(4206..5168)
                     /gene="pfkA"
                     /locus_tag="ACHLAM_002817"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO7854318.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5349..6251)
                     /gene="fieF"
                     /locus_tag="ACHLAM_002818"
     CDS             complement(5349..6251)
                     /gene="fieF"
                     /locus_tag="ACHLAM_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO7854319.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6400..6900)
                     /gene="cpxP"
                     /locus_tag="ACHLAM_002819"
     CDS             complement(6400..6900)
                     /gene="cpxP"
                     /locus_tag="ACHLAM_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO7854320.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7050..7748
                     /gene="cpxR"
                     /locus_tag="ACHLAM_002820"
     CDS             7050..7748
                     /gene="cpxR"
                     /locus_tag="ACHLAM_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO7854321.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7745..9118
                     /gene="cpxA"
                     /locus_tag="ACHLAM_002821"
     CDS             7745..9118
                     /gene="cpxA"
                     /locus_tag="ACHLAM_002821"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO7854322.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9224..9898)
                     /gene="yiiM"
                     /locus_tag="ACHLAM_002822"
     CDS             complement(9224..9898)
                     /gene="yiiM"
                     /locus_tag="ACHLAM_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO7854323.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGVSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10047..11030)
                     /gene="kdgT"
                     /locus_tag="ACHLAM_002823"
     CDS             complement(10047..11030)
                     /gene="kdgT"
                     /locus_tag="ACHLAM_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO7854324.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11290..11910)
                     /gene="sodA"
                     /locus_tag="ACHLAM_002824"
     CDS             complement(11290..11910)
                     /gene="sodA"
                     /locus_tag="ACHLAM_002824"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO7854325.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12195..13229
                     /gene="rhaT"
                     /locus_tag="ACHLAM_002825"
     CDS             12195..13229
                     /gene="rhaT"
                     /locus_tag="ACHLAM_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO7854326.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(13226..14074)
                     /gene="rhaR"
                     /locus_tag="ACHLAM_002826"
     CDS             complement(13226..14074)
                     /gene="rhaR"
                     /locus_tag="ACHLAM_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO7854327.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14148..14984)
                     /gene="rhaS"
                     /locus_tag="ACHLAM_002827"
     CDS             complement(14148..14984)
                     /gene="rhaS"
                     /locus_tag="ACHLAM_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO7854328.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARYLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15272..16741
                     /gene="rhaB"
                     /locus_tag="ACHLAM_002828"
     CDS             15272..16741
                     /gene="rhaB"
                     /locus_tag="ACHLAM_002828"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO7854329.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTHGLMAQAQRQLGKCDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQSNEIPVVAVASHDTASAVIASPLNSSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            16738..17997
                     /gene="rhaA"
                     /locus_tag="ACHLAM_002829"
     CDS             16738..17997
                     /gene="rhaA"
                     /locus_tag="ACHLAM_002829"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO7854330.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            18182..19006
                     /gene="rhaD"
                     /locus_tag="ACHLAM_002830"
     CDS             18182..19006
                     /gene="rhaD"
                     /locus_tag="ACHLAM_002830"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO7854331.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNKAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            19016..19330
                     /gene="rhaM"
                     /locus_tag="ACHLAM_002831"
     CDS             19016..19330
                     /gene="rhaM"
                     /locus_tag="ACHLAM_002831"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO7854332.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(19371..20765)
                     /locus_tag="ACHLAM_002832"
     CDS             complement(19371..20765)
                     /locus_tag="ACHLAM_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO7854333.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATSESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         5205 a         5474 c         5197 g         5029 t
ORIGIN      
        1 catcctgggt atctttacca tatttatttt cgccttgtgt gccaacaaac gcaccaagat
       61 cgataagcat catcaccaga atcaatgtcg ggacaaaacg ccccaccgcc cattgccaga
      121 cacccggtaa aattgcccag ttacccgcca gcagcatcca cgccacaatc atcagaaatg
      181 cccatgcgcc ggaacgcccg cgatcatgca agcgcttaac ggttactgcc gctgttggcc
      241 aaagcaagca cacaaggcaa aacgccgcgg tctgaatatc gagtaaattt ttacccgcca
      301 gtgagaaaag caccagcatg cctgcgaacc acaggcctat ccaaatccag aaatcacggc
      361 gtccaatacg ccctttaaat gagaataacc attgctgtat ggtcatgtaa gttccttgat
      421 ggttgtcttt tccaggattc tacccttttg acaagggcga cagatatcgt tttaatcgga
      481 gccagtcata acaaaaggta ctgtcaatga agccagggtg tacgctgttt tttctcttat
      541 gttctgcatt aaccgttaca acaacggcgc atgcacaaac accagatacg gcaacgaccg
      601 cgccttatct gctggctgga gcccctactt tcgatctctc catcagccag tttcgagaag
      661 actttaacag ccagaatccc agcctgccac tgaacgaatt tcgtgccatc gacagcagtc
      721 ccgacaaagc caatctcact cgtgcagcaa gtaaaattaa cgagaacttg tatgcttcta
      781 ctgcgctgga gcgcggtacc ttaaaaatca aaagcattca aatgacctgg ctacccatcc
      841 aggggccaga gcaaaaagcc gcgaaagcga aagctcaggc atacatggca gcggtgatcc
      901 gcacactcac cccattaatg accaaaactc aaagccagaa aaaactgcag gcgctactaa
      961 cggcggggaa aaacaaacgt tattacaccg agacagaagg tgcactgcgt tatgttgtcg
     1021 cggacaacgg cgaaaagggg ctgaccttcg ctgttgaacc gattaagctg gcgctatctg
     1081 aatcgcttga aggtttgaat aaatgacaaa aagcaaagcc tttgtgccga tgaatctcta
     1141 tactgtttca cagacctgct gccctgcggg gcggccatct tcctttattc gcttataagc
     1201 gtggagaatt aaaatgcgac atcctttagt gatgggtaac tggaaactga acggcagccg
     1261 ccacatggtt cacgagctgg tatctaacct gcgtaaagag ctggcaggtg ttgctggctg
     1321 tgcggttgca atcgcaccac cggaaatgta catcgatatg gcgaagcgtg aagctgaagg
     1381 cagccacatc atgctgggtg cgcaaaacgt ggacctgaac ctgtccggcg cattcaccgg
     1441 tgaaacctct gctgctatgc tgaaagacat cggcgcacag tacatcatca tcggccactc
     1501 tgaacgtcgt acttaccaca aagagtctga cgaactgatc gcgaaaaaat tcgcggtgct
     1561 gaaagagcag ggtctgactc cggttctgtg catcggtgaa accgaagctg aaaacgaagc
     1621 gggcaaaact gaagaagttt gtgcacgtca gatcgacgcg gtactgaaaa ctcagggtgc
     1681 tgcggcattc gaaggtgcgg ttatcgctta cgaacccgta tgggcaatcg gtactggcaa
     1741 atctgcaact ccggctcagg cacaggctgt tcacaaattc atccgtgacc acatcgctaa
     1801 agttgacgct aacatcgctg aacaagtgat cattcagtac ggcggctctg taaacgcgtc
     1861 taacgctgca gaactgtttg ctcaaccaga catcgacggc gcgctggttg gcggtgcttc
     1921 tctgaaagct gacgctttcg cagtgatcgt taaagctgca gaagcggcta aacaggctta
     1981 agtctgacag gtgccggatt tcatatccgg cacttacttt ccttaactcc tcgccttaac
     2041 gcaaaatctc acactgatga tcctggattt cctcggctga agcacggtta agcgtcagta
     2101 gatttcgttg tgtcgccagc aatacaaatg agttatcact ctgccgcacc atcgccagcc
     2161 cgtagcttcc catatgttcc cgcgcctcag gcacttcttc tgcaagcatc ataaatgggc
     2221 tgcgttgtac cagttcgctt tccgttaccc gacgcgccag gtattcatgc ccgcgcaaac
     2281 cacctggcag tggcaaccag cggctgctga tgttcgccag attgttatcc agctgttcgc
     2341 gcacatcagg acgaatacaa gagatatgaa tatgaaaatg gttttgcgta cgcccggtgc
     2401 gagagttgat cgccaaagaa accgcgcgat cgggaaccgg ctggccgtat tttttgctca
     2461 taaaatcacg cgcctgccag gccaaccaaa agaagttcgg cgttgaagga tcggtcaaca
     2521 aaggactttc agtaccgtta atacgatacg ttggcatcaa cagatattgc agtgggccat
     2581 taagatcttt taaaaccacg tatccggcat tgggtttgac ttccgcacat ggcgaaggat
     2641 tttgattttg ctgctgattg ggcaaacatt cctcaaggac aatcttacgt aatgtatccg
     2701 actcttcacc ggttaatttc cagtaaccaa taccggcagc cacaacggcg ataactatca
     2761 tcaccaaaaa aagaagaccc gcttttttca tcttttttcc ctgtacctca aagagatgca
     2821 agggtaacgc aaaatcgtga caaataaaaa acccagtcgc gagaaaccac cgggtttgat
     2881 aattatcctg gagaaatcag cgtttgctaa tctgatcgaa cgtaccgccg ttagcaaaat
     2941 gctctttttg cgctttcgtc cagccgccga actcttcatc aatggtgaat aacttcagct
     3001 ttggaaacgc attttcgtac tttttcgcca cctcagcatc gcgcggacgg tagtagtttt
     3061 tcgcggcaat ttcctgacct tctggcgagt agagatattt caggtaggct tccgccacct
     3121 ctttggtgcc ttttttctcg accactttat cgaccaccga cacggttggc tctgcgagga
     3181 tagactcact cggcgtgaca atttcgaatt tatctttccc cagttcattc gctgccagca
     3241 gagcttcgtt ttcccaggca atcagtacat cgccaatccc gcgctcgaca aaggtattag
     3301 tggagccgcg cgcgccagaa tccagaactt cgacgttttt atacagtgcc cgaacaaaat
     3361 cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag gctgccaggt
     3421 agttccagcg cgcgccacca gagcttttcg gattaggcgt gatcaccgaa acacccggtt
     3481 taatcagatc gttccagtca tggatctgct tcggattgcc cttacgcacc aggaaaacaa
     3541 tggtggaagt gtacggtgcg gagttatccg gcagacgttt gatccactct ttatcaatcc
     3601 gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc cagcgtgaca acatcagctt
     3661 caataccgtt gattaccgac gtcgcttgtt tgcctgaacc accgtgggac tgacggatca
     3721 ccacgttatc gccagtttgc tgtttccagt gggcgctgaa taccttgttg tactgttcgt
     3781 acaattcgcg cgttggatca tatgaaacgt taagaagctg aatatccttt gccataacgc
     3841 tggttgccgc cagcaaaaat gttaacccta cgccccactt gttcatcgcc cgactctctt
     3901 atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac caaacattaa agaataaaaa
     3961 aagattggct ataacttgcg ggtatatgta gggggattaa aaaggcggaa gaaatccgcc
     4021 tcatattgct gacaaagtgc gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg
     4081 cctacaaaag ttcgcaaatt caataaattg cagaattcat gtaggcctga taagcgaagc
     4141 gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc ctttttccga
     4201 aatcattaat acagtttttt cgcgcagtcc agccagtcgc ctttgaacgg acgcttcatg
     4261 ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctggat acctacgcaa
     4321 cgaccaccgt aacctgccag cagcagctcg atagcgtaag cgcccatacg ggaagccaga
     4381 atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac agttgcgcgg
     4441 gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt
     4501 tcggtaatcg ccactatcgc gtgtttttta cctttcgcga tacccgcttt gatttcgttt
     4561 accaggtctt cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca gccaccggca
     4621 atagccgcag ccaaagtcag atcgccacaa taacggccca tcacttccac cacggaaata
     4681 cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc
     4741 gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc
     4801 agaccgatgc acgggaagcc catttcggtc agacgcattg cacccatgta ggaaccgtca
     4861 ccgccgataa ccaccagcgc gtcgatacca cgttttttca ggttttcgat agccacggcg
     4921 cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt accaccacgg
     4981 ttgatcatgt cagaaacgct gtaacggtct agctgtacca tacggtcttc atacagaccc
     5041 agatagccgt cataaatgcc cattacttcc agaccttctg tcagcgcaga acgaacaacc
     5101 ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac accgattttc
     5161 ttaatcatga ctacctctga actttggaat gcaaaatgaa atctgttgcc ggaagtcttc
     5221 ttgcacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt cctgctgaat
     5281 tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct ggctctcttt
     5341 tatactgatt atgaaagcat agaccgttta ccctccctgg gtacgacgga acaggggtcc
     5401 tgatggataa tcacatccga tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc
     5461 gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg aatctgaata
     5521 aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac acccggccag
     5581 gaagtcacga tatcaataat ttcttgccgt tcttcatcag gcaatgcgcg atccagtaat
     5641 gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat gccgattccc
     5701 aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag cgccagcaga
     5761 attgcaccgt tcatcataac atcagactgg taatgtagca tatcagcccg caccgcctgg
     5821 ctttgcgtcc ggcgcaccac ccaacgctga aacgagacaa ggataatcgt acaaattagc
     5881 gccacaattg tcacgataac cccgacgcct ggatctgtca tcggtgttgg agatatcaga
     5941 tgttgaatac ccgtcaaaaa caggaatagt gctgaaccgg agataaacat actttgcgcc
     6001 agcgccgcga gggactcagc tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc
     6061 agggaatatc gcaccaccag taaattcgtc aacgacgcgc cgatatccac cagcgaatcc
     6121 accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat tttaatcagc
     6181 aatagcagtg aagccatcgc cgttgcagca atcgccgccc gactgaccag ccgtccataa
     6241 gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag caaatcatct
     6301 gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa cccccacatc atgtggggga
     6361 agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct
     6421 actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc
     6481 cattcgttgt tgatgtttct catttaaaac cgcttgctgc tccggcgtta acaggcgata
     6541 catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt
     6601 ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa ggcgatgcat
     6661 tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc
     6721 tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat ggctctgcgt
     6781 actgcgctgc gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc
     6841 gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat
     6901 catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg
     6961 caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac
     7021 gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc
     7081 gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg
     7141 cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg
     7201 atgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc
     7261 agacgcctgt cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg
     7321 agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta
     7381 ttcgcgcgat cctgcgccgt tcgcactgga gcgaacaaca gcaaaacaac gacaacggtt
     7441 caccgacact ggaagttgat gccttaagtc tcaatcctgg tcgccaggaa gccaccttcg
     7501 acgggcaaac actggagtta accggtactg agtttaccct gctctatttg ctggcacagc
     7561 atctgggtca ggtggtttcc cgtgagcatt taagccagga agtgctgggc aagcgcctga
     7621 cgcctttcga ccgcgctatc gatatgcata tttccaacct gcgtcgtaaa ctgccggatc
     7681 gtaaggatgg tcacccgtgg tttaaaaccc tgcgtggtcg tggctatctg atggtttctg
     7741 cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggcgct
     7801 ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct
     7861 ggatagcgaa cagcgtcagg gtctgatgat tgagcagcat gttgaagcgg agctggcgaa
     7921 cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc
     7981 accgccagga cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg
     8041 cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca
     8101 gaagaaaaag tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga
     8161 taattaccaa ctttatctga ttcgtccggc cagcagttct caatccgatt tcataaactt
     8221 actgtttgac cgcccgctat tactgctgat tgtcaccatg ctggtcagta cgccgctgct
     8281 gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga
     8341 agttgcccaa ggaaacttac gccagcatcc ggaactggaa gcggggccac aggaattcct
     8401 tgccgccggt gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca
     8461 gcaacgtcta ctttctgata tctctcacga actgcgcacc ccgctgacgc gtctgcaact
     8521 gggtacggcg ttactgcgcc gtcgtagcgg tgaaagcaag gaactggagc gtattgaaac
     8581 cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg gtgatgtcac gtaatcagca
     8641 gaaaaatgcg ctggttagcg agaccatcaa agccaaccag ttgtggagtg aagtgctgga
     8701 taacgcggcg ttcgaagccg agcagatggg caagtcgttg acggttaact tcccgcctgg
     8761 gccgtggccg ctgtacggca acccgaacgc cctggagagt gcgctggaaa acattgttcg
     8821 taatgccctg cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg
     8881 tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat
     8941 tttccgtccg ttctatcgta ccgatgaagc acgcgatcgt gaatctggcg gtacaggttt
     9001 ggggctggcg attgtagaaa ccgccattca gcagcatcgt ggctgggtga aggcagaaga
     9061 cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac
     9121 tccgcatttg taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggatgcct
     9181 gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg ggattatttt ccccacaatc
     9241 ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtt cgcgtccagc
     9301 ttttcgatac ccctgcggcg gagagtaaac ggtgatactg atcgtcatca aacggcatat
     9361 gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct gacacgggaa accaactcca
     9421 gtggcgcatc agcagatacc aaccctggtg caattacgct atacaaccac cccactttac
     9481 ccgtgttttg catcaactgc gctatatcgc tgatatcaaa atggtaattg agtttatagc
     9541 aaggtgagcg tggctggctg acctgaatta atgcctctcc ccagcggaaa atatcgccaa
     9601 tataaacatt actttccgtc agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa
     9661 caaacaactc cgcctgttcc ggaaattccc gcgcccagta aagataatgc tcacgaggat
     9721 aatgacacag cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt
     9781 ccagccccag gtccgtcagc atcaactcac catcaacctg gattttggca attgcgctgg
     9841 gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct
     9901 gttccctctc cacgagaaat ttccccatta gaccatattt attttatgtc aggcgtgctg
     9961 ttttcgcggt aagtatccag gcaggaactc aatagcaaac cggacgaacg ttagttcatc
    10021 cggctgtggg gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct
    10081 gctctggctt tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc
    10141 acaatgaccg ccgtcgcgac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc
    10201 accatttctg caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca
    10261 attccggcag tgccatcgcc accgccaatc aatttatcag cgataatcaa cggaataccg
    10321 gtcacgataa ttactgccac acccaacagg atccccagca aacctgtctg ggcaattacc
    10381 gtcaaatcaa tggtattacc cagtgcgaag gcaaagaatg gaatcagcgt ctgcaccgct
    10441 ttgctgaaaa attctcgtaa ttcagggtca aggttcccaa gggcaaagcc caccaggaac
    10501 ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga
    10561 ataatcatcg tcatgagcgg cccggactcc aatgacatca acacaaatgc cccagcttct
    10621 tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc
    10681 gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc
    10741 ggaataatgc gcgaggcaat tgccgcaacc acccacgcga cggcaatttt tgttaccacc
    10801 agcgtaccgg atttacgcaa taccgttccc gtcgcgctta attttattga cgcccccatg
    10861 caaaaaaacc acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat
    10921 ccaaaatatt tccccgcctc cggcgagaag gtgtggcaca gtgcgccaag gaataacggg
    10981 acgagcatca tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc
    11041 tgtcggcata tttttaaggg ctgatgcgtc attcctcaat ggagcatcag tgttgatgga
    11101 aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca attgaaacac
    11161 tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa aaaccaccgc
    11221 cgttggcgat ggttcatttt ttaagctgat atgcggcgta aacgcctcat tgcagcaggc
    11281 ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcgtcccagt tcaccacgtt
    11341 ccagaactct ttgatgtagt caggacggcg gttctggaat ttcaggtagt aagcatgttc
    11401 ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag
    11461 cggggaatcc tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca
    11521 tgcccagccg gaaccaaagc gggaagctgc cgctttctca aattctgctt tgaagttatc
    11581 aacggagccg aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt
    11641 tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac
    11701 ggttttcttg tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt
    11761 ggcaaattct ggcaggcttt ccagcgccgc gttggcgttg ttgacgtagg tctgatggtg
    11821 tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc
    11881 atacggcagg gatggcaggg tataactcat attcatctcc agtgttgtcg ggcggccgat
    11941 tgttaatgcc gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt
    12001 atcaatgccg tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc
    12061 aaatcaccca cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag
    12121 gttaatcgac agcacgattt ttacactcat ctcgtcggag atgtgacgcg acgaaaaatg
    12181 atgaggataa gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg
    12241 cggccagtgc agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa
    12301 ccatgtggtc agttggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc
    12361 tgttactacc gaatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg
    12421 tttttctgtt cggcgcaatg tgggggattg gtaatatcaa ctacggcctg accatgcgtt
    12481 atctcggcat gtcgatgggg attggtatcg ccattggtat cacgttgatt gttggtacgc
    12541 tgatgacgcc aattatcaac ggcaatttcg atgtgttgat caataccgaa ggtggacgca
    12601 tgacattgct cggcgttctg gtggcgctga ttggtgtagg gattgtgact cgcgccgggc
    12661 agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt caacctgaaa aaagggctgg
    12721 tgctggcggt gatgtgcggc attttctctg ccggaatgtc ctttgcgatg aacgccgcaa
    12781 aaccgatgca tgaagccgct gccgcactgg gcgtcgatcc actgtatgtc gctctgccaa
    12841 gctatgttgt catcatgggc ggcggcgcga tcattaacct cggtttctgc ttcattcgtc
    12901 tggcaaaagt gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca
    12961 ttcacaatgt gttactctca gcactgggcg ggttgatgtg gtatctgcaa ttctttttct
    13021 atgcctgggg ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata
    13081 tgagcttcta tgtattgtgc ggcggtatcg tcgggttggt gctgaaagag tggaacaatg
    13141 caggtcgccg tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcca
    13201 acatcgtcgg catgggcatg gcgaattaat ctttctgcga attgagatga cgccactggc
    13261 tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc
    13321 cacattcggt tgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat
    13381 gacacactct gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt
    13441 gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg
    13501 gacttttcag gctggccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg
    13561 ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga
    13621 acagcaactc tgccatttcg ttagcaaacg gcacatgctg actactttca tgctcaagct
    13681 gaccgataat ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc
    13741 ctgcgctggc actaaatccc ggaatcgccc cctgccagtc aagattcagc ttcagccgct
    13801 ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat
    13861 cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga tcgttgagta
    13921 catgcagacc attaccgcgc cagacaatta ccagctcaca aaaatcatgt gtatgttcag
    13981 caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa
    14041 aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa
    14101 gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaaa
    14161 ccatcccgtc cctgacgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc
    14221 gaaaagtggt tactgtcgct gaatccgcag cgataggcga tgtcagtaac gctggcctcg
    14281 ctgtggcgta gcagatatcg ggctttcatc agtcgcaggc ggttcaggta acgctgaggc
    14341 gtcagccccg tttgctgctt aagctggcga tgcagcgtac gcaatgaaag agaaaattga
    14401 tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc
    14461 aagttgagac gtgacgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag
    14521 agcagtaatt gcataaataa gatctcgcga ctggcggtcg agggtaaatc attttcctct
    14581 tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaacacgct gtggttaacg
    14641 cgccagtgag acggatactg cccatcctgc tcttgtggca gcaactgatt cagcccggcg
    14701 agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag attatcggta
    14761 tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta
    14821 tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga
    14881 aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac
    14941 gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt
    15001 cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggga
    15061 aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga
    15121 gcatcacacc accacaattc agcgaattgt gaacatcatc acgttcatct ttccctggtt
    15181 gccaatgacc cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac
    15241 tggtcgtaat gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga
    15301 tctcggcgca tccagtggac gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct
    15361 gacgctgcgc gaaatccatc gttttaacaa tgggctgcat agccagaacg gttatgtcac
    15421 ctgggatgtg gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga
    15481 agggattcgt atcgatagca ttgggattga tacctggggc gtggactttg tgctgcttga
    15541 ccaacagggt cagcgcgtgg gcctgcctgt tgcttatcgc gatagccgca cccatggtct
    15601 gatggcgcag gcacaacggc aactcggcaa atgcgatatt tatcaacgta gcggcatcca
    15661 gtttttgccc ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac aacctgaact
    15721 tattccacac attgctcacg ctctgctgat gccggattac ttcagttatc gtctgaccgg
    15781 caagatgaac tgggaatata ccaacgccac gaccacgcaa ctggtcaata tcaatagcga
    15841 cgactgggac gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc
    15901 gacgcatccg ggtaatgtca ttgggcactg gatttgcccg cagagtaatg agattccggt
    15961 ggtcgccgtt gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacagttc
    16021 acgcgccgct tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc
    16081 atttaccaat gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg
    16141 ctatcgggtg ctgaaaaata ttatgggctt atggctgctt cagcgggtgc ttcaggagcg
    16201 gcaaatcaac gatcttccgg cgcttatcgc cgcgacacag gcacttccgg cttgccgctt
    16261 caccatcaat cccaatgacg atcgctttat taatcctgac gagatgtgca gcgaaattca
    16321 ggctgcgtgt cgggaaacgg cgcaaccaat cccggaaagt gatgctgaac tggcgcgctg
    16381 tattttcgac agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcaactgcg
    16441 cggtgaagat ttctcacaac tgcatattgt cggcggtggc tgccagaacg cgctgctcaa
    16501 ccagttatgt gctgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcgac
    16561 gctcggcaat atcggcatcc agttaatgac gctggatgaa ctcaacaatg tggatgattt
    16621 ccgtcaggtc gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga
    16681 aattgcccac tatgtggcgc agattcactc tacacgacag acaaaggagc tttgcgcatg
    16741 accactcaac tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc agtagggatt
    16801 gatgtcgagg aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag
    16861 ggcgatgatg tttccggttt tgaaaacccg gaaggttcgc tgaccggtgg gattcaggct
    16921 actggtaatt atccgggcaa agcgcgtaat gccagcgagc tacgtgccga tctggaacaa
    16981 gctatgcggc tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatca
    17041 gatacgccag tcgcgcgcga ccaaatcaaa ccagagcact tcaaaaactg ggttgaatgg
    17101 gcgaaaacca atcagctcgg tctggatttt aacccctcct gtttttcgca tccgctaagc
    17161 gccgatggct ttacgctttc ccatgccgac gacagtattc gccagttctg gattgatcac
    17221 tgcaaggcca gccgccgtgt ttcagcctat tttggcgagc aactcggcac accgtcggtg
    17281 atgaacatct ggatcccgga tggcatgaaa gatatcaccg ttgaccgtct cgccccgcgc
    17341 cagcgtctgc tggcagcact ggatgaggtg atcagcgaga agctggatcc ggcgcatcat
    17401 atcgatgccg ttgagagcaa attgtttggc attggcgcgg agagctacac ggttggctcc
    17461 aatgagtttt acctggggta tgccaccagc cgccagacgg cgctgtgcct ggacgccggg
    17521 catttccacc cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtgccg
    17581 cagttgctgc tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg
    17641 gatgatgaaa cccaggcgat tgccagtgag attgttcgtc acgatctgtt cgaccgggtg
    17701 catatcggcc tcgacttctt cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt
    17761 acacgcaata tgaaaaaagc cctgctacgt gcgttactgg aacctaccgc tgaactacgc
    17821 aagctggaat cggcgggcga ttacactgcg cgtctggcgc tactggaaga gcagaaatcg
    17881 ttgccgtggc aggcggtatg ggaaatgtat tgtcagcgtc acgatacgcc agcgggtagc
    17941 gaatggctgg agaacgtgcg ggcttatgag aaagaaattt tgagtcgccg cgggtaaaca
    18001 ctgccggatg cggcacaagt gccttatccg gcctacgggt cggcaatagt tgtaggcctg
    18061 ataagacgcg acagcgtcgc atcaggcatt gattgccgga tgcggcacga gtgccttatc
    18121 cggcctacag atcgtatatc cggcaacagg cacggacgtc caattaacag gaacaccgaa
    18181 catgcaaaac attactcagt cctggtttgt ccagggaatg attaaagcca ccaccgacgc
    18241 ctggctgaaa ggctgggacg agcgcaacgg cggcaacctg acgctgcgcc tggatgacgc
    18301 cgatatcgca ccatatcacg acaattttca ccaacaaccg cgctatatcc cgctcagcca
    18361 gcccatgcct ttactggcaa atacaccgtt tattgtcacc ggctcaggca aattcttccg
    18421 taacgtccag cttgatcctg cggctaactt aggcatcgta aaagtcgaca gcgacggcgc
    18481 gggctaccac attctttggg ggttaaccaa caaagccgtc cccacttccg aacttccggc
    18541 tcactttctc tcccactgcg agcgcattaa agccaccaac ggtaaagatc gggtgatcat
    18601 gcactgccat gccaccaacc tgatcgccct tacctacgta ctggaaaacg acaccgcggt
    18661 cttcactcgc caactgtggg aaggcagcac cgaatgtctg gtagtgttcc cggatggcgt
    18721 cggcattttg ccgtggatgg tgcccggcac cgacgaaatc ggccaggcga ccgcacagga
    18781 aatgcaaaaa cattcgctgg tgttgtggcc cttccacggc gtcttcggca gcggtccgac
    18841 actggatgaa acattcggtt taatcgacac cgcagaaaaa tcagcacaag tattagtgaa
    18901 ggtttattcg atgggcggca tgaaacagac catcagtcgt gaagagctga tagcgctcgg
    18961 ccagcgtttc ggcgttacac cactcgccag tgcgctggcg ctgtaaggag caaacatgat
    19021 ccgcaaagcc tttgtcatgc aggttaaccc cgacgcccac gaagagtatc agcgtcgtca
    19081 taatccaatc tggccggaac tggaagcagt gttgaaatct cacggtgcgc ataactacgc
    19141 catttacctc gacaaagcgc gtaatctgct gtttgccacg gtagagattg aatctgaaga
    19201 acggtggaat gcggtagcca gtactgatgt ttgccaacgt tggtggaaat atatgaccga
    19261 cgttatgcca actaacccgg ataacagccc agtcagtagc gagctgcaag aagtgtttta
    19321 cctgccttaa tgagttgcgg tgccggaaaa tatccagcac cgcgacttca tcagaagaag
    19381 tatttaaagc gaacgcgtgc accgtaacgg tcatcagtat catcaccgtg ttttttatca
    19441 ccgtcgctat ccagaatgga gtaatacgtc ccgaggtaaa tattaaagtt ctccatatcc
    19501 atcacattag cgaactgata agacgcatga atggtgtgaa tggtgtacgt cccttcatca
    19561 tcaatccagc agtcgttatc acaaactaca ccgctgaatt catcaatctt attgtgcgcg
    19621 tagatataac ccagctcgaa acgtttccac agcgcgttaa tcccggcagt gaaatctttc
    19681 tcattattag catctaaata ggcggtatta agattaacca cgacaccatt ttccggatcc
    19741 gttttcaggc cattccaggt catgctcata ccatagccgg tacgatcgga ctgatcgaca
    19801 aaattaccct tgctatcggt ataaccataa gcattattta ccacgttcgc ttccatcgct
    19861 gcggaaacgg tgaattcttc cgtcggcgac caggcaatta ccggacgcag ataagcaaca
    19921 tttttctgct gttccatatc gcgtccatga taattaccgt cgttatataa agatgttccg
    19981 tcttccagta acgtgtttaa ttcaaaatac cagttatcga gttgtttgct gacgaggaaa
    20041 ttaccgccag cgttagaacg cccgcggccc tctttcatca tatagatata accgctgcca
    20101 tcgtcataaa gatcgttcgc agtattaccg gaatgttcaa caaaggtatc ctgattcagc
    20161 gggaacatat cgtaggcttc aaaacggcct actttgacct tccagtcgtt ttctttgcca
    20221 aagaagaata ccgcatcatc gatatttact gaaccgtgca tgtcccccag cggttgtgcg
    20281 gagaacccgg cgaaataacc gttgtccatc tttcgcatac cgtcaaaacc taacagaata
    20341 cgaccattga gattccactg ttcattagtg ggatcgctat tcacatcagc gttggtcatt
    20401 gccagaaggc catgattact ttccgcatcc atattaaatt ctacgtcgcc gtagattttt
    20461 aaatcgccat aacctgaaag caaaagatta ggtgaggtgg aatttttctt tgcagattcc
    20521 gacgttgcct gtccatttac cggcgtattg ccctgggcag ccttaatttc gcggatctca
    20581 gcggcttgtt gtgtctgcag ctgtttaacc tggctctctg cttttgctgc ccgcatttct
    20641 gccgcttcca ggcgttcttc cagcaatgcc atgcgttgtt ccagcgttaa atgctgtgtt
    20701 tttgccattc cggtactaca tgccaataat aaaccgatgg ctaaagataa tgtcgatttt
    20761 ttcattattt acgttccctg aaataaaaga gcccctacgc tttccaaaag gaatacgcat
    20821 atagagaaat ataaggtgtg cactttcgaa tttttaatcc aacttcagca ggccgatgga
    20881 tagtatcccg ccatataata tgagt
//
LOCUS       DAZGAP010000090        20736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGAP010000090 DAZGAP010000000
VERSION     DAZGAP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.090"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(168..803)
                     /gene="nth"
                     /locus_tag="ACHLAM_002833"
     CDS             complement(168..803)
                     /gene="nth"
                     /locus_tag="ACHLAM_002833"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO7854334.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(803..1498)
                     /gene="rsxE"
                     /locus_tag="ACHLAM_002834"
     CDS             complement(803..1498)
                     /gene="rsxE"
                     /locus_tag="ACHLAM_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO7854335.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(1502..2122)
                     /gene="rsxG"
                     /locus_tag="ACHLAM_002835"
     CDS             complement(1502..2122)
                     /gene="rsxG"
                     /locus_tag="ACHLAM_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO7854336.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(2126..3184)
                     /gene="rsxD"
                     /locus_tag="ACHLAM_002836"
     CDS             complement(2126..3184)
                     /gene="rsxD"
                     /locus_tag="ACHLAM_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO7854337.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(3185..5407)
                     /gene="rsxC"
                     /locus_tag="ACHLAM_002837"
     CDS             complement(3185..5407)
                     /gene="rsxC"
                     /locus_tag="ACHLAM_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO7854338.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKACKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(5400..5978)
                     /gene="rsxB"
                     /locus_tag="ACHLAM_002838"
     CDS             complement(5400..5978)
                     /gene="rsxB"
                     /locus_tag="ACHLAM_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO7854339.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISYNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(5978..6559)
                     /gene="rsxA"
                     /locus_tag="ACHLAM_002839"
     CDS             complement(5978..6559)
                     /gene="rsxA"
                     /locus_tag="ACHLAM_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO7854340.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFFQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(6636..7076)
                     /gene="ydgK"
                     /locus_tag="ACHLAM_002840"
     CDS             complement(6636..7076)
                     /gene="ydgK"
                     /locus_tag="ACHLAM_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO7854341.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(7162..7377)
                     /gene="ydgT"
                     /locus_tag="ACHLAM_002841"
     CDS             complement(7162..7377)
                     /gene="ydgT"
                     /locus_tag="ACHLAM_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO7854342.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(7650..7775)
                     /gene="blr"
                     /locus_tag="ACHLAM_002842"
     CDS             complement(7650..7775)
                     /gene="blr"
                     /locus_tag="ACHLAM_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO7854343.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            8018..9058
                     /gene="ydgJ"
                     /locus_tag="ACHLAM_002843"
     CDS             8018..9058
                     /gene="ydgJ"
                     /locus_tag="ACHLAM_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7854344.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEGWGYDMRD
                     GVLTRVEGEERIEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(9094..10095)
                     /gene="add"
                     /locus_tag="ACHLAM_002844"
     CDS             complement(9094..10095)
                     /gene="add"
                     /locus_tag="ACHLAM_002844"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO7854345.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10199..11371)
                     /gene="malY"
                     /locus_tag="ACHLAM_002845"
     CDS             complement(10199..11371)
                     /gene="malY"
                     /locus_tag="ACHLAM_002845"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO7854346.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFASAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(11381..12973)
                     /gene="malX"
                     /locus_tag="ACHLAM_002846"
     CDS             complement(11381..12973)
                     /gene="malX"
                     /locus_tag="ACHLAM_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO7854347.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            13148..14176
                     /gene="malI"
                     /locus_tag="ACHLAM_002847"
     CDS             13148..14176
                     /gene="malI"
                     /locus_tag="ACHLAM_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO7854348.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            14288..15055
                     /gene="hdhA"
                     /locus_tag="ACHLAM_002848"
     CDS             14288..15055
                     /gene="hdhA"
                     /locus_tag="ACHLAM_002848"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO7854349.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            15276..15866
                     /gene="uidR"
                     /locus_tag="ACHLAM_002849"
     CDS             15276..15866
                     /gene="uidR"
                     /locus_tag="ACHLAM_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO7854350.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLSREASFAQGLRAMIGGILTAS"
     gene            16253..18064
                     /gene="uidA"
                     /locus_tag="ACHLAM_002850"
     CDS             16253..18064
                     /gene="uidA"
                     /locus_tag="ACHLAM_002850"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO7854351.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQEIRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            18061..19434
                     /gene="uidB"
                     /locus_tag="ACHLAM_002851"
     CDS             18061..19434
                     /gene="uidB"
                     /locus_tag="ACHLAM_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO7854352.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            19473..>20736
                     /gene="uidC"
                     /locus_tag="ACHLAM_002852"
     CDS             19473..>20736
                     /gene="uidC"
                     /locus_tag="ACHLAM_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO7854353.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         5204 a         5140 c         5398 g         4994 t
ORIGIN      
        1 aatagaaaat ttcgctatta aaaaaacaat taacctgcat ttttggttat ccgcaaacgg
       61 attacctgat gataacgcta agcaatggca tcattgacgt taaaaagatg ttttaaaaga
      121 ggataaagaa aggttatcaa tggggtaatc ggtgttaccc cttttcttca gatgtcaact
      181 ttctctttgt attcacaaag atcttcaata atacaagagc cacagcgagg cttgcgggca
      241 atacaggtat aacgcccgtg caggatcaac caatggtggc agtcgacttt aaactctgcc
      301 ggaaccactt tcagtagctt ttcttctacc tgttcgacgt ttttccccgg cgcaaattga
      361 gtacgattac aaacgcggaa aatgtgcgtg tcgacagcaa tagtaggcca gccgaatgca
      421 gtgtttaata cgacgttggc tgttttacga cctacaccag gcagggcttc aagcgcagca
      481 cgatcttccg gaacctcgcc attatgctgt tccagtaaga tacggcaggt tttgatgata
      541 ttttctgctt tgctgttata aagcccaatg gttttgatat aggttttcac cccttcgacg
      601 cccagttcaa gcatcgctgc aggtgtatta gccaccgggt agagtttcgc cgtcgcctta
      661 ttaacactga catcggtcgc ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc
      721 gaactgaaat taagctcggt ggtgggatga ggattgttct cacgcaggcg agtgaggatc
      781 tccaggcgtt ttgctttgtt catcagacat tccctgtttc accgtttggc aatgcacgtt
      841 cggcagctgc ttcagcacgg cgctttttca ttctttcatc aatcaggtat ttccctgcca
      901 gcatcagtcc caggccaata aatgcacctg gtggcagcat cgccagcagg aaaggggagt
      961 cggtgtggaa tatctccacg cgtaatactt ttgcccagct acctaacagc gcatctgcac
     1021 cgtcaaacaa tgtgccattg ccgataattt cgcgtagtga acccagcacg aacatggcgc
     1081 aggttgcgcc cataccaatt gaaaagccgt ccagtgccga aagcgccgga ccttttttgg
     1141 cggcgaaggc ttcagcgcgg cccacaacga tacagttagt gacaatcagc ggaataaaaa
     1201 tccctaatga ttgatacagg ccaaaggcgt aggcgttgat cagcatctgt acagcgctga
     1261 ccaccgaggc gatgatcatc acgtaaatgg gaatgcggat ctcggctggc gtccagtgac
     1321 gcagcgtcga aatggtcaag ttggtcagcg tcagtaccag cgtagtcgca agtcctaaac
     1381 ccagagcgtt agtggcagtg gacgtgaccg ccaatagcgg acagaggccg agcaactgga
     1441 ccagcgcaga gttgtttttc cacaacccct gaacaataac gtctttaatt tcgctcatgc
     1501 tttattctcc acaggcagga agttgagaaa gttgtgccgg taacgtctga gcgtacaatc
     1561 cggcgcgttt taccgcattt accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt
     1621 cgaaatcacc accatctttc ttcaccgccc agtttgcatc atccgcacca ctgatttttt
     1681 tacccgcaaa atgggtgatc cagtcagaaa ggcgcagttc gattttatcg ccaagccctg
     1741 gcgtttcgtg gtgctctgtc acgcgcgtgc caagtaccgt gccgttaaaa tcggctccca
     1801 ccagcagctg aatcgcaccg gaatagccat ctggcgcggt tgtttccaga acggcggcta
     1861 ccggtttgtc atcctgtttg gcgatgtaaa cccgatgctc acctttacct aactctggcg
     1921 cagtgaccag atagcaactc tgtgccagcg cattgttata gcgttcggct ggcagcacct
     1981 gatcaaataa cgccttttgt tgcagactgg cctgttcagc aattgtcgtt ttggtcatct
     2041 ggttgatggc cgcagttaac cctgtagaac ccgctgcaaa tagtgccagt gtaatgccgt
     2101 gttttcggat agttttcagc atggtttacc ctttgcgatg gccgtagacg cgcggacgcg
     2161 tgtagtaatc gatcagagga accgtgatgt tcgccagcag gacggcaaaa gccacgccgt
     2221 caggatagcc gccgaaactg cggatcaacc agactaataa acccgccagc gcgccgaaca
     2281 tcagacgacc acgattggtc gtagaagcag taaccgggtc agtcaaaata aagaatgcgc
     2341 cgagcatagt cgcaccagac agcagatgaa tttgcggtga tgccagtgtt tctggtgaga
     2401 acaaccagcc caacgttgcg cataacgcca gcgttactaa gaagctgagg ggaatatgcc
     2461 agcgaatcgc tttctgccat agcaaccaca cgccgccagc cagccaggcg agatttaccc
     2521 attgccaacc aacgcccgcc agcataccgc tgtagatcgg atattgcata atctgttcaa
     2581 ccgaatgacc ggcacggaca gaggttttaa atgtatccaa cggtgtcgcc tgactaatgc
     2641 catcaatacc taagcgtagc gtgttcatat caccaccact ggtggtatgt ccgctgaaaa
     2701 taacctgtat ggcgtcgata aaaccaggga tgttgaccgc aatttcatgt ggcggtaacc
     2761 agctggtcat ctgcaccggg aaggagatca gtaagaccac ataaccaatc attgccggat
     2821 taaacgggtt ttgccccaga ccgccataca actgtttagc aatgatcacc gcaaacaccg
     2881 tacccagcac gaccatccac catggcgcga gggggggaat acttaccgcc agcaataagc
     2941 ctgtcagcaa tgctgagtta tctttcaacg ttgcggctac cgactgcttg cgtagtttga
     3001 gtacgagagc ttcggctaac agagcactga ccgacgccaa caggatctga acgagagtac
     3061 cccaaccaaa aaaccacagt tgcgctgcga ttcctggcac ggctgcgagc aacaccaaca
     3121 gcataatgcg cgatgtttgg cgctggttat gggtataagg ggagctagct attctgaata
     3181 ccatttagtc ctcgtttaca accttttgct gggcggcttt tttggcctga acgcgggcaa
     3241 tagccgcagc aactgccgct ttgcgcggat caatctgttc ttctggctcc gcattagcct
     3301 gttgctgttc cagcttgcgt gctttggcac gggcaatagc agcttcgacg gcggctttgc
     3361 gcggatcaac ctgttcttct ggttctgcgt tagcctgttg ctgttccagc ttgcgcgctt
     3421 tggcacgggc aatagccgct tcgacggcag ctttgcgcgg atcgacctgt tcttctggtt
     3481 ccgcattagc ctgttgctgt tccagcttgc atgctttggc acgggcaata gcggcttcga
     3541 cggcggcttt gcgcggatcg acctgttgtt ctggctcggc attagcctgt tgctgctcca
     3601 gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc ggctttgcgc ggatcgacct
     3661 gttcttctgg ttccgcatta gcctgttgct gttccagctt gcgcgctttg gcgcgggcga
     3721 tagcggcttc aacggcagtt ttacgtggat cagcaacggt tgctgcgtcg ttagtttgct
     3781 gcagttctgc ctgtttcgct ctggcttgcg ctttacgggc ttcccgtgct gcaataattg
     3841 cactgttatc cgggcgttcg cccgctttaa tcacaatagg ctgtgtagcc tgggcctgtt
     3901 tctctttcac ccgcgccaga gcggcagcaa tcgcatcttt atctttggct gcaggttgaa
     3961 cggctgcgct cttatgtcgt tcaaggcgag ccgctttttc gcgctccaga cgagcctggc
     4021 gcgcttcgaa acgcgctttg gcttctgcgg cgcgcttttc ttcctgacga atagctgcaa
     4081 tttcagcttt ttcctgacgg aaatattgca ccaggggaat attgctcggg caaacccacg
     4141 cgcaagcccc acattcaatg cagtcggcaa tgttatgcgt ggtagcttta tcgtgttgct
     4201 gacctttgct gaaccagtac aactgttgcg gcaaaagatc cgcagggcag gcgtcagcac
     4261 aggcgctaca ccggatgcag ctttgttctt cctgtggttc gccaagctca ttggcagagg
     4321 gagccaacag acagttggta atctttacga ccgggacatc cagccatggc aaggtaaagc
     4381 ccattagcgg gccgcccata atcaccattt gatcggcaga ggggcagaat ccggcgtcat
     4441 tcaataaatg acgcactggc gtccccagcc gtgcccagac gttgcccggg cgagcgattg
     4501 cttcgccagt cagggttacg acacgctcgg taatcggctc gccatcaata acggcacgtt
     4561 tcactgcata agcagtgccg acgttttgca ttaatacgcc gatatcggat gaacgcccgc
     4621 catgtggaac ctgcttcccg gtcagaatgt aggttaattg tttagcaccg ccagaaggat
     4681 atttggttgg aatcacccgc agagaaatat cgtgagagtc cgccagcacc gcgcgcagca
     4741 tggaaatcgc ctgcggtttg ttatcttcaa tgccgataag aatttcgcgt ggctgcagaa
     4801 tatgcgcaag aatgcgaata ccctctacga cctgagccgc gcaatcctgc atcaaacggt
     4861 catcggcggt aatgtacggc tcgcactcag cagcgttgat aatcaacgtt tcaatcttat
     4921 ctccgccacc ctgcaattta acgcctgtcg ggaatcctgc accgcccagc ccggcaacac
     4981 caaactgatg aatgcgctcg attaactctt cgcgactgcg agagcgataa tcggcccagc
     5041 cgtctcgcgg gatccagcag tcttcaccat cggcatcaat aatcacgctt aattcagcta
     5101 acgctgaagg atgagccgta gagtggggcg caatagccgt aacggtaccc gaggtgggcg
     5161 cgtgaacagg cagcattttg ccgcgaccac gggtaagcgg ctggccgcgc aatactttat
     5221 cgccgacgct aacgcacaac tcaccttcag cgccaatatg ctgtttcagt ggaataacaa
     5281 aacgctgcgc cagtggtacc tggcgcaggg gtgtaccgtt ggactgggtt ttcatctccg
     5341 gtggatggat gccgccgttg aaatcccaga ttttattttt tctgaatgca gagaataact
     5401 taagcatggt gttccacggg aatgatacgc acgggaatgg tgttcagatc ccatttccag
     5461 gagtcaggtg tttctgcgac cggttgcaac gagatgcagt gcgtcgggca cggatcaaca
     5521 cataaattgc agcccgtaca gagatcactc attaccgtat gcatggcacg ggtagcgcca
     5581 acgatggcgt ctaccggaca agcctgaata catttagtgc agccaataca gttattttca
     5641 tcaataaccg ccaccatccg cgcaggcgtt agctcttgcg cttcgccatc cagcggctgc
     5701 ggctcgacat taagcaactc ggcaattttt agcatcacag cttcgccacc aggggcgcaa
     5761 cggttgattt tttcaccgtt atagctgatg gcttccgcgt aggggcgaca gccgggataa
     5821 ccgcactgac cacactggct ctgcggtaag atttcgtcaa ttttctcaac gaccggatcg
     5881 tcttccaccg caaaacggcg ggaggcataa cccagaatgg cgccaaacgc caggcccagc
     5941 aggctcacgg cggcaacggc aatccagata gcattcatta caacttcacc aaaccactaa
     6001 agcccataaa ggccagagac ataagacctg cggtaattaa tgcaatggca ttaccgcgaa
     6061 aaggtgccgg gacatcagcc acagcaaggc gttcgcggat ggcggcgaag agcaccatca
     6121 ccagcgagaa accgacagcg gcggaaaaac cgtacagcgc cgactgaaag aaattgtgcc
     6181 cgagattgat attcagcaac gccacgccga gcactgcaca gttggtggtg ataagcggca
     6241 aaaaaatccc cagcagacgg taaagcaccg ggctggtttt gcgcaccacc atctcggtga
     6301 actgcacaac cacagcaatc accagaataa acgccagggt gcgcaggtaa atcagattaa
     6361 gcgggatcaa aatccacgta tcgataagcc aggcgcaaat agacgccagc gtcatcacaa
     6421 acgttgtagc cagccccatg cccatcgcgg tttccagctt tttggaaacc cccataaacg
     6481 gacagagacc gagaaacttg accagtacaa agttattgac cagtacagtt ccgacaaaca
     6541 gtaacaggta gtcagtcatt tttcggcctg aaataaaaaa gccgcctatt atccgttaat
     6601 ccagagcagg cgacaacagg ttaactgtaa ggttattacg gattcacaaa cgtcgctttc
     6661 acccgcgacg agcgtttaaa ataggggacg atcaatgcgg ttgccaacaa agtaaacagt
     6721 aactggcgaa cggctatgcc atcttccacg ggtgaaaaag cgaacgcttt tactgccagc
     6781 aatacggaaa taagcagcca gataatatag tgtttaggaa cacaacgccg acgtttgaag
     6841 aaagcgatgg tcaaccacag tgtgtaatac cacatggcga tagcggtaat aaaagaaacg
     6901 ccccataaga tttgtgtcgt cagtgcttgt tcgccgagcg tttgaaatgt ttgcggagaa
     6961 gataacgcag cggtatacag caacaacgcc agcgtcgtac ttaacagagc gactaaaagc
     7021 caggccaatg ggccaagcaa ccagcctcca atacgttgtg gcgttgtggt ggtcataggt
     7081 tctccataat agcggcgcaa acaggcaaat ctaattttag gggcgttatt ataaggcttt
     7141 tataaccgat cgctactttc tttattggac atagtgccag acggacttcg gtacctggcc
     7201 gaggtcaaac aaacgcccgc cagaaaccag ctctgcgcga cggtgatcgg cagcacgata
     7261 catattgatc agttcctgat cgtccgtcag ggtgtaatta agatggtcgt agagtttttc
     7321 caaactttcg agtgaactga ttttgcgaaa ttttaataag tagtcctgaa cagtcataaa
     7381 aggtccataa aataattaat accaaagggg taggtattat tagtgatgat aaaatttcac
     7441 caaaagtgaa cgcttgtgtt attacaataa taagaatgcg ctgatggcaa atattcaggg
     7501 atgaaaaact gctctgaaaa gagtaacgcg ctttactatc tctgacaatc accaacaaca
     7561 tcgaacaaga taataaattc ctggtttaat atccgacaag tgaaaacatg cacccggacg
     7621 ggcagcatgt cgctctacaa gcgcagagct tacttgtgtt gtaccgtagt actctgttca
     7681 ggtggctgat agttgtcaat gtgactcgcc acgccaagaa gaatgactga aacgacaaga
     7741 acgatccaac ctgttaattc aataagacga ttcattacag cccacatctc tgttgattga
     7801 tccattaact tcagggggta aatgttactt agcaataata gctccagcaa gatttttact
     7861 gaggtttttg cgatatttag cttttgtcgt tggaaaattc gctatttttt tgacttaagt
     7921 taaacaacat cccttattgc tggcaggtta ttaaactgtt gagcgagggt aaaggatagt
     7981 gtcaaatagc catcatactt caatgagagg caatgacatg agcgacaaca tccgtgttgg
     8041 gttgattggg tatggttatg cgagcaaaac cttccatgcg cccctgattg cgggcacgcc
     8101 cgggctggaa ctggcggtaa tctccagcag cgatgaaaca aaagtaaaag ccgactggcc
     8161 aacggttacg gttgtctctg agccgaagca tctgtttaac gatcccaaca tagacctgat
     8221 tgtcattcct acacccaacg atacccattt cccgttagcc aaagcggcgc ttgaggcggg
     8281 taaacatgtg gtcgttgata aaccctttac cgtgacactg tcacaagcgc gagagctgga
     8341 agcgctggca aaaagcctag ggcgtgtgct gtctgtattc cataaccgtc gctgggatag
     8401 cgatttcctg acgctaaaag gtttgctcgc ggaaggcgtg ctgggtgaag ttgcttactt
     8461 tgagtctcat tttgaccgct tccgtccgca ggttcgcgat cgttggcgtg aacagggcgg
     8521 cccaggcagc ggtatctggt acgatttagc accgcacctt cttgatcagg ccattacgct
     8581 atttggttta ccggtcagca tgacggtaga tttggcacag ttacggcccg gagcgcagtc
     8641 gaccgattat ttccacgcca tcttgtccta tccgcagcgg cgagtcattt tacacggtac
     8701 catgctggca gctgctgagt cagcacgtta tatcgtgcat ggatcccgag gcagttatgt
     8761 gaaatatggc ctcgatccac aggaagaacg actgaaaaat ggcgagcgtc tgccgcagga
     8821 aggctggggc tacgatatgc gtgatggcgt acttacccgc gtggaaggtg aggaacgtat
     8881 cgaagaaacg ctgttgacag taccagggaa ttatccggct tactatgcgg ctattcgtga
     8941 tgcgttaaat ggagatggtg aaaatccggt tccggcaagt caggcaatcc aggtaatgga
     9001 gttgattgag ctgggcatcg aatccgccaa acatcgcgcg acgctgtgcc ttgcgtgaaa
     9061 agaggatggt gcaattagca ccatccattt ttgttacttc gcggcgactt tttctcgcag
     9121 tgcgcgtttt tcctctgcgc tgaggaaagc catttccaga ccattaatct gcgcctggcg
     9181 gatttgctcg cgggataacc cagcagccgg cgcggcaacg gtatattcgt gaatgatatc
     9241 cactccctgt acgccgggat cgtcagtgtt aatgctggca cgaatgccat gctcaaggaa
     9301 cgttttcagc ggatgtgccg ccagctctgc cacggtgctg gtctgaatat tggaggtcag
     9361 acaggattca ataccaattt gctgctcggc gagaaaatcc atcagcgccc gatcttcaat
     9421 ggcttttacg ccatgtccaa tacgttccgc ccccagttca cgaatcgcct gccagatgct
     9481 ttccggcccg gcagcttcgc ctgcatggac ggtaatatgc cagcccgcat cacgcgcgcg
     9541 gttgaagtga gaaaggaaca gacttcccgg gaaaccaagt tcatcaccgg ctaaatcaag
     9601 tgcggtaatc tggtcacggt gggctaaaaa ggcctccagc tcttgctgac aggcggcttc
     9661 gccgaaggtc cggctcataa tgccgataag cttcgcctgc acaccaaagg tgcggcaacc
     9721 ttcacgtacg ccatcgatca ccgcttcgac aacacccgct acaggcagct gatgtgccat
     9781 tgccatgtag cccggtgaaa aacgcagctc gacatagtgc aggccattac gggctgcatc
     9841 ttcaatgttt tcaaatgcca cgcggcggca agcatcaaga gaggcgagaa ctttaacgcc
     9901 ccagtcaagt ttggtcagaa agctcaccag atcgggttcg ttggcaatga cctgaacgtg
     9961 gggaatcagt gtttccaggg attgtgcagg aagcgagata ttatactggc ggccaagttc
    10021 aagaatggtc tggggacgaa tgttgccatc aaggtggcga tggatatcag ttaatggcag
    10081 ggtggtatca atcatggtcg cactcttttt ctggatataa aagtgcgcac cattataaaa
    10141 acaaagcagg gtaaaaatcc acgattgcgc aacgattttt tacgttgcgc aattggggtt
    10201 aacgaacagc gcggatggcg ttaattaatc cagcaacacc tttttccagt ttcgaacgcg
    10261 ggcagccggc attgagacgg acaaaaccac gaccttcttc accgtaggta taccccggca
    10321 tgatcgcgac cttctcttgc tcgataagcg ctttttgcaa cgcgttgtcg tcaatattca
    10381 atggacgtag atcaagccag gccagataag tggattgcgg gatctgccag ttgagttcag
    10441 ggaacgcggc gttcatttta tctgcgatat acgtcaggtt atctttcaga tagacgcgta
    10501 aggcatccag ccacggtgcg ccttgctgat aggcggcgat atgagcagtt aacgccagta
    10561 ccgaagggga agaaagccca tcacgtcctt tcagtgccga taaataggca tcacggctgc
    10621 tgctattttc tataatcccg taagcaccgg tcagggcggg aatattgaaa cttttcgatc
    10681 ccgacgttag caatgcccag tctccgcgcg ccacattact ccagggaata tgcggctgct
    10741 cgccccaaac catatccata tggatttcat cggaaataac ccgcacacca tgacgctcgc
    10801 acaggtcagc catgatctcc agttcatcgc acgtccacac tttcccggta ggattctgtg
    10861 ggctacacag gagcataatt ttacattctg gtttcgccaa cacggcttcc agcttgccca
    10921 tatcgcaaaa ccagccgtca gcccgcttct ctaaagcgac gggcattact gtgcgctggt
    10981 taccttcaat ggccttgtaa aatgcgtcat aggcgggtgt gtggatcacc acgccttcac
    11041 ctttttcaga ccactgacga atcagttccg agaccatgta gataacagaa gggccataca
    11101 ccaccgactg cgtatcaatt gcggtgtaat gctgggtgga aaaccagtgg gcaatagccg
    11161 cgagaaactc atcgtttttc cagcggctgt agccaaacac gccgtgcatc aggcgctgat
    11221 tcagcgcctc gataatgcag ggggcagagg caaaatccat gtctgaaatc gtgaacggta
    11281 acaggtcagc agtgccgaaa cggtcagcga cataatccca ctgcgtacac catgtgccat
    11341 gacgatccac gacctttgaa aaatcgaaca tatcttatcc ttatgcctgg acagtatgca
    11401 tcagaccggc catttcatct tttactgact gaacttgtgg cccgataaca acctgcaggt
    11461 tatgttgatt aagttgtact acgccaattg cccgattgtc cttcagtgcc tgcacattaa
    11521 caagcgacat atctttcaca gacaaacgca gacgggtaat gcagttatcg aggctgacaa
    11581 tattgtcggc accgcctaag gcttcgagga ttgccggaac gttgtaacct gatttacccg
    11641 gcgcaccggc aacggctttt tcgattgagc tggcaacttc gctatcgcgc cccggggttt
    11701 tcagattgaa gcgggtgata gcgaaacgga agatgacgta gtaaacgaca aaccagattg
    11761 ccgccaccac tggcaccatg taccacttgg ttgacagacc atgcaaaata ccgaacacca
    11821 cgaagtcgat gatattgccg tcggtattac cgatggtgac gccgagcaca gacatgacgg
    11881 tgaagccgag gccggttaac agcgcgtgga tgacatacag aactggcgct acgaacagga
    11941 acaggaattc cagcggttcg gtcgtgccgc caacgacaca ggcgatcagg ccagaaataa
    12001 gcagaccttt aattttatgg cgattttccg ggcgcgcgca gtgatacata gctaacgctg
    12061 cacctggcag gccgccgaga aacgcaggca ttttaccttg cgaaaggaaa cgcgtggcgc
    12121 tttcagaaaa accgtgagtg gtcgggcaac tcaattgcgc ctggaagatg gtcagtgcgc
    12181 cgctgacggt ttgaccgcag acttcctgcg tgccgcctgc gtcggtaaag cgaattaatg
    12241 ccaccagaat gtgatgcaga ccaaacggca acagcagacg ttcaccggta ccaaacagca
    12301 tcggtccgaa atcacccgcg ctgttgatca tatggcctaa gccgctaata cccatggcga
    12361 aaattggcca gactaatgga atcaccagac cgacaaggcc catcaccagc gaggagatga
    12421 ttggcacgaa gcgcgtaccg ccgaagaatg ccagcgcatc cggcaggcgg atattatgga
    12481 aacgctcatg gagcatccag acgataatac cggcgatcac cgcaccgagg atccctgtat
    12541 cgatcgactg gatcccaaga atgctctgaa tgttattggc tttcagaacc gcggcgtccg
    12601 tggttggcag aatgccttta ttggtcaacc agaagtttac cgcgagattc attaccgcat
    12661 aaccgacaaa gccagcaaat gccgctacac ctttgttttc gcgtgccagg cccagcggga
    12721 tggcgataca gaacatgact ggcaggaaac taaaagcaaa cgagccaatc ttactcatcc
    12781 aggtaaagat agcctgcaac acggggttgc ccaggaccgg gatcagggtg atgacatcat
    12841 ggctgctaag agaactacca atgccgagca taatgccgca gaacgacaat aatgccacag
    12901 gtagcatgaa ggttttgcct aactgctgga agaactccca cagcgtgact ttcggtgctg
    12961 ttttcgccgt cataaaacga ctcctcgtag agaacaatct ggattcagta aattgcgcga
    13021 gaagataaaa cgttttatca aattttagtg aggcataaat cacattacgc aacgataata
    13081 gcgggtataa gataaataaa aggtaaaacg ttttatctgt cacataatca gggagtaggt
    13141 catctgcatg gctaccgcca aaaaaataac cattcatgat gttgcgctgg ctgcgggcgt
    13201 gtcggtaagc accgtttcgc tggtgctaag tggcaaaggg cgaatctcta ccgccacagg
    13261 agaacgcgtt aacgccgcca ttgaagagct gggatttgtg cgcaatcgtc aggcgtcggc
    13321 gctgcgcggc gggcaaagcg gcgtcattgg tttgatcgtc cgtgatttat ctgcgccgtt
    13381 ttacgccgaa ttaacggccg gattgacgga agctctggaa gcgcagggac ggatggtttt
    13441 tttgcttcac ggcggtaaag acggcgagca gctggcacag cggttttcac tgttactgaa
    13501 tcagggggtc gatggtgtgg tcattgccgg ggctgcagga agcagcgatg acctgcgacg
    13561 gatggcagaa gaaaaagcta tcccggtgat tttcgcttcc cgtgccagtt atcttgatga
    13621 tgttgatacg gttcgcccgg ataacatgca ggctgcacag ttgttgacgg agcatctcat
    13681 tcgcaatggg catcagcgta tcgcctggct gggagggcaa agttcctcat taacccgggc
    13741 agaacgggtg gggggctatt gtgcaactct actaaaattt ggcctgccgt ttcacagcga
    13801 ttgggtgttg gagtgctctt ccagccagaa gcaagccgcg gaagctatca cggcgctttt
    13861 acgtcataac ccgaccatca gtgctgtggt ttgctataac gaaactattg cgatgggggc
    13921 atggtttggt ttgctgaaag cagggcggca aagcggggaa agcggagtcg atcgttactt
    13981 tgaacaacag gtttcgttgg cggcatttac cgatgcaacg ccaaccacac ttgatgatat
    14041 acctgttacc tgggccagca cgcctgcgcg ggaacttggt accacacttg cggatcgcat
    14101 gatgcaaaaa atcacccatg aagagacgca ttcacgcaat cttattattc ccgcccggct
    14161 cattgcggcg aaataatcct ctctttatct gctatacctg gtagtgtcct ttcctcaagg
    14221 ttaatgataa aaaatgtggc acacaggcgg tgccttttat ttttactgaa gttcacagga
    14281 ggtttatgtg tttaattctg acaacctgag actcgacgga aaatgtgcca tcatcacagg
    14341 tgccggtgca ggtattggta aagaaatcgc cattacattc gcgacagctg gcgcatctgt
    14401 ggtggtcagt gatattaacg ccgacgcagc taaccatgtt gttgatgaaa ttcaacaact
    14461 gggtggtcag gcatttgcct gccgttgtga tattacttcc gaacaggaac tctctgcact
    14521 ggcagacttt gccgtcagta agctgggtaa agttgatatc ctggttaaca acgccggtgg
    14581 cggtggtcct aaaccgtttg atatgccaat ggcagatttt cgccgtgctt atgaactgaa
    14641 tgtgttttct tttttccatc tgtcacaact tgttgcgcca gaaatggaaa aaaatggcgg
    14701 tggcgttatt ctgaccatca cttctatggc ggcagaaaat aaaaatataa acatgacttc
    14761 ttatgcatca tctaaagctg cggccagtca tctggtcaga aatatggcgt ttgacctggg
    14821 tgaaaaaaat attcgggtga atggcattgc gccgggggca atattaaccg atgccctgaa
    14881 atccgttatt acaccagaaa ttgaacaaaa aatgttacag cacacgccga tcagacgtct
    14941 gggccaaccg caagatattg ctaacgcggc gctgttcctt tgctcgcctg cagccagctg
    15001 ggtaagcgga caaattctca ccgtctccgg tggtggggta caggagctca attaatacac
    15061 taacggaccg gtaaacaacc gtgcgtgttg tttaccggga taaactcatc aacgtctcag
    15121 ctaaataact ggcagccaaa ttacggctat tggttaacca atttccgagt gaaaagtata
    15181 cgaatagagt gtgccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg
    15241 gtaaactagt tattcacgct aactataatg gtttaatgat ggataacatg cagactgaag
    15301 cacaaccgac acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat
    15361 ttcacagtgc ctcgatgaaa gccatctgta aatcttgcgc cattagtccc gggacgctct
    15421 atcaccattt catctccaaa gaagccttga ttcaggcgat tatcttacag gaccaggaga
    15481 gggcgctggc ccgtttccgg gaaccgattg aagggattca tttcgttgac tatatggtcg
    15541 agtctattgt ctctctcacc catgaggcct ttggacaacg ggcgctggtg gtcgaaatta
    15601 tggcggaagg gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga
    15661 tcacggaatt tgttgcccag cggatgcgtg atgcccagca aaaaggcgag ataagcccag
    15721 acatcaacac ggcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg
    15781 atatcgaagc ggaagatctg tcgcgtgaag cgtcgtttgc tcagggattg cgggcgatga
    15841 ttggcggtat cttaaccgca tcctgatcct ctcatttcgc gggctggtga taactgtgcc
    15901 cgcgtttcat atcgtaattt ctctgtgcaa aaattatcct tcccgggttc ggagaattcc
    15961 ccccaaaata ttcactgtag ccatatgtca tgagagttta tcgttcccat tatgctcgaa
    16021 cgaacgttcg gttgcttatt ttatggcttc tgtcaacgcc gttttaaaga ttaatgcgat
    16081 ctatatcacg ctgtgggtat tgcagttttt ggttttttga tcccggtgtc agttcttttt
    16141 atttccattt ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta
    16201 actaatcaga ttaaaggttg accagtattt ttatcttaat gaggagtccc ttatgttacg
    16261 tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac ggtctgtggg cattcagtct
    16321 ggatcgcgaa aactgtggaa ttgatcagcg ttggtgggaa agcgtgttac aagaaatccg
    16381 ggcaattgct gtgccgggaa gttttaacga tcagttcgcc gatgcagata ttcgtaatta
    16441 tgtgggcaac gtctggtatc agcgcgaagt ctttataccg aaaggttggg cgggccagcg
    16501 tatcgtactg cgtttcgatg cggtcactca ttacggcaaa gtgtgggtaa ataatcagga
    16561 agtgatggag catcagggtg gctatacgcc gtttgaagcc gatgtcacgc cgtatgttat
    16621 tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaat gaactgaact ggcagactat
    16681 cccgccggga atggtgatta ccgatgaaaa cggcaagaaa aagcagtctt acttccatga
    16741 tttctttaac tatgccggga tccatcgcag cgtaatgctc tacaccacgc cgaacacctg
    16801 ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga
    16861 ctggcaggtg gtagcaaatg gtgatgtcag cgttgaactg cgtgatgcgg atcagcaggt
    16921 ggttgcaact ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca
    16981 accgggtgaa ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga
    17041 tatctacccg ctgcgcgtcg gcatccggtc agtggcagtg aaggacgaac agttcctgat
    17101 taaccacaaa ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgcgg
    17161 caaaggattc gataacgtgc tgatggtgca cgatcacgca ctaatggact ggattggggc
    17221 caactcctac cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga
    17281 acatggcatc gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat
    17341 tggtttcgaa gcgggcaaca agccgaaaga actgtacagc gaagaggcag tcaacgggga
    17401 aacccagcag gcgcacttac aggcgattaa agagctgatt gcgcgtgaca aaaaccaccc
    17461 aagcgtggtg atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga
    17521 atatttcgcg ccactggcgg aagcaacgcg taaactcgat ccgacgcgtc cgatcacctg
    17581 cgtcaatgta atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct
    17641 gtgcctgaac cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa
    17701 ggttctggaa aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac
    17761 cgaatacggt gtggatacgt tagccggcct gcactcaatg tacaccgaca tgtggagtga
    17821 agagtatcag tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgctgt
    17881 cgtcggtgag caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt
    17941 tggcggtaac aagaaaggga tcttcactcg cgaccgcaaa ccgaagtctg cggcttttct
    18001 gctgcaaaaa cgctggaccg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca
    18061 atgaatcaac aactctcctg gcgcgccatc gtcggctaca gcctcggtga cgtcgccaat
    18121 aacttcgcct tcgcaatggg ggcgctcttc ctgttgagtt actacaccga cgtcgctggc
    18181 gtcggtgccg ctgcggcggg caccatgctg ttactggtgc gggtattcga tgccttcgcc
    18241 gatgtctttg ccggacgagt ggtggacagt gtgaataccc gatggggaaa attccgcccg
    18301 tttttactct tcggtactgc gccgttaatg atcttcagcg tgctggtatt ctgggtaccg
    18361 accgactgga gccatagcag caaagtagtg tatgcatatt tgacttacat gggcctcggg
    18421 ctttgctaca gcctggtgaa tattccttat ggttcacttg ctaccgcaat gacccaacaa
    18481 ccacaatccc gcgcccgtct gggcgcggct cgcgggattg ccgcttcatt gacttttgtc
    18541 tgcctggcat ttctgatagg accgagcatt aagaactcca gcccggaaga gatggtgtcg
    18601 gtatatcatt tctggacaat tgtgctggcg attgccggaa tggtgcttta cttcatctgc
    18661 ttcaaatcga cgcgtgagaa tgtggtacgt atcgtggcgc agccgtcatt gaagatcagt
    18721 ctgcaaaccc tgaaacggaa tcgcccgctg tttatgttgt gcatcggtgc gctgtgtgtg
    18781 ctgatttcga ccttcgcggt cagcgcctcg tcgttgttct acgtgcgcta tgtgttaaat
    18841 gataccgggc tgttcactgt gctggtactg gtgcaaaacc tggtcggtac tgtggcatcg
    18901 gcaccgctgg tgccggggat ggtcgcgagg atcggtaaaa agaatacctt cctgattggc
    18961 gctttgctgg gaacctgcgg ttatctgctg ttcttctggg tttccgtctg gtcactgccg
    19021 gtggcgttgg ttgcgttggc catcgcatca attggccagg gcgttaccat gaccgtgatg
    19081 tgggcactgg aagctgatac cgtagaatac ggtgaatacc tgaccggcgt gcgaattgaa
    19141 gggctcacct attcactatt ctcatttacc cgtaaatgcg gtcaggcaat cggaggttca
    19201 attcctgcct ttattttggg attaagcgga tatatcgcca atcaggcgca aacgccggaa
    19261 gttattatgg gcatccgcac atcaattgcc ttagtacctt gcggatttat gctactggca
    19321 ttcgtcatta tctggtttta tccgctcacg gataaaaaat tcaaagaaat cgttgttgaa
    19381 attgataatc gtaaaaaagt tcagcagcaa ttaatcaacg atatcactag ttaatattca
    19441 ataaaaataa tcagaacatc aaaggtgcaa ctatgagaaa aatagtggcc atggccgtta
    19501 tttgcctgac ggctgcctct ggccttacct ctgcttacgc ggcgcaaccg gctgacgatg
    19561 aagcgggact acgcatcaga ctgaaaaacg aattacgcag ggctgataag cccagtgctg
    19621 gcacgggaag agatatttac gcatgggtac agggaggatt gctcgatttc aatagtggtt
    19681 attattccaa tattattggc gttgaaggcg gggcgtatta tgtttataaa ttaggtgctc
    19741 gtgctgatat gagtacccgg tggtatcttg atggtgataa aagttttggc tttgccctgg
    19801 gggcagtaaa aataaaaccc agtgaaaata gcctgctaaa attaggtcgc ttcgggacgg
    19861 attatagtta tggtagctta ccttatcgta ttccgttaat ggctggcagt tcgcaacgta
    19921 cattaccgac agtttctgaa ggagcattag gttactgggc tttaacgcca aatattgatc
    19981 tgtggggaat gtggcggtca cgagtatttt tatggaccga ttcaacaacc ggtattcgtg
    20041 atgaaggggt gtataacagc cagacgggaa aatacgataa acatcgcgca cgttcttttt
    20101 tagccgccag ttggcatgat gataccagtc gctattctct gggggcatcg gtacagaaag
    20161 atgtttccaa tcagatacaa agtattctcg agaaaagcat accgcttgac ccgaattata
    20221 cgttgaaagg ggagttgctc ggcttttacg cgcaacttga aggtttaagt cgtaatacca
    20281 gccagcccaa tgaaacggcg ttggttagtg gacaattgac ctggaatgcg ccgtggggaa
    20341 gcgtatttgg cagtggtggt tatttgcgcc atgcaatgaa tggtgccgtg gtggataccg
    20401 acattggcta tcccttttca ttaagtcttg atcgtaaccg tgaaggaatg cagtcctggc
    20461 aattgggtgt caactatcgt ttaacgccgc aatttacgct gacatttgca ccgattgtga
    20521 ctcgcggcta tgaatccagt aaacgagatg tgcggattga aggcgcgggt atcttaggtg
    20581 gtatgaacta tcgggtcagc gaagggccgt tacaagggat gaatttcttt cttgctgccg
    20641 ataaagggcg ggaaaagcgc gatggcagtg cgctgggcga tcgcctgaat tactgggatg
    20701 tgaaaatgag tattcagtat gactttatgc tgaagt
//
LOCUS       DAZGAP010000091        20436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGAP010000091 DAZGAP010000000
VERSION     DAZGAP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.091"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..855
                     /locus_tag="ACHLAM_002853"
     CDS             <1..855
                     /locus_tag="ACHLAM_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO7854354.1"
                     /translation="IKKHGIALSILLIPCIGNAQEIKIDESWLHQSLNVIGRTDSRFG
                     PRLTNDLYPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFS
                     IDKLTGLNLAFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANI
                     YAKYQWQNYGAANENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKSHN
                     NKRTSNAIASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGT
                     YTTIGYQF"
     gene            complement(1072..3504)
                     /gene="metL"
                     /locus_tag="ACHLAM_002854"
     CDS             complement(1072..3504)
                     /gene="metL"
                     /locus_tag="ACHLAM_002854"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO7854355.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3507..4667)
                     /gene="metB"
                     /locus_tag="ACHLAM_002855"
     CDS             complement(3507..4667)
                     /gene="metB"
                     /locus_tag="ACHLAM_002855"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO7854356.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4944..5261
                     /gene="metJ"
                     /locus_tag="ACHLAM_002856"
     CDS             4944..5261
                     /gene="metJ"
                     /locus_tag="ACHLAM_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO7854357.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5321..5929
                     /locus_tag="ACHLAM_002857"
     CDS             5321..5929
                     /locus_tag="ACHLAM_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO7854358.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5990..6202)
                     /gene="rpmE"
                     /locus_tag="ACHLAM_002858"
     CDS             complement(5990..6202)
                     /gene="rpmE"
                     /locus_tag="ACHLAM_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO7854359.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6405..8603
                     /gene="priA"
                     /locus_tag="ACHLAM_002859"
     CDS             6405..8603
                     /gene="priA"
                     /locus_tag="ACHLAM_002859"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO7854360.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8759..9784
                     /gene="cytR"
                     /locus_tag="ACHLAM_002860"
     CDS             8759..9784
                     /gene="cytR"
                     /locus_tag="ACHLAM_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO7854361.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9876..10835
                     /gene="ftsN"
                     /locus_tag="ACHLAM_002861"
     CDS             9876..10835
                     /gene="ftsN"
                     /locus_tag="ACHLAM_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO7854362.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKIAGHTNCIRLAAGG"
     gene            10928..11458
                     /gene="hslV"
                     /locus_tag="ACHLAM_002862"
     CDS             10928..11458
                     /gene="hslV"
                     /locus_tag="ACHLAM_002862"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO7854363.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11468..12799
                     /gene="hslU"
                     /locus_tag="ACHLAM_002863"
     CDS             11468..12799
                     /gene="hslU"
                     /locus_tag="ACHLAM_002863"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO7854364.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12866..13792
                     /gene="menA"
                     /locus_tag="ACHLAM_002864"
     CDS             12866..13792
                     /gene="menA"
                     /locus_tag="ACHLAM_002864"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO7854365.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13885..14370
                     /gene="rraA"
                     /locus_tag="ACHLAM_002865"
     CDS             13885..14370
                     /gene="rraA"
                     /locus_tag="ACHLAM_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO7854366.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(14455..14700)
                     /gene="zapB"
                     /locus_tag="ACHLAM_002866"
     CDS             complement(14455..14700)
                     /gene="zapB"
                     /locus_tag="ACHLAM_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO7854367.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            15126..15971
                     /gene="glpF"
                     /locus_tag="ACHLAM_002867"
     CDS             15126..15971
                     /gene="glpF"
                     /locus_tag="ACHLAM_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO7854368.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15994..17502
                     /gene="glpK"
                     /locus_tag="ACHLAM_002868"
     CDS             15994..17502
                     /gene="glpK"
                     /locus_tag="ACHLAM_002868"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO7854369.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17673..18683
                     /gene="glpX"
                     /locus_tag="ACHLAM_002869"
     CDS             17673..18683
                     /gene="glpX"
                     /locus_tag="ACHLAM_002869"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7854370.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18780..19526
                     /gene="fpr"
                     /locus_tag="ACHLAM_002870"
     CDS             18780..19526
                     /gene="fpr"
                     /locus_tag="ACHLAM_002870"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO7854371.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(19531..19959)
                     /gene="uspD"
                     /locus_tag="ACHLAM_002871"
     CDS             complement(19531..19959)
                     /gene="uspD"
                     /locus_tag="ACHLAM_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO7854372.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(19986..20285)
                     /locus_tag="ACHLAM_002872"
     CDS             complement(19986..20285)
                     /locus_tag="ACHLAM_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO7854373.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         4964 a         5340 c         5382 g         4750 t
ORIGIN      
        1 ataaaaaagc acgggatagc tttatccatt ctattaatac cttgcattgg aaacgctcag
       61 gaaattaaaa ttgatgaaag ctggttacat caaagcttga atgtcattgg tcgcacagac
      121 tctcgctttg gcccaagact gactaacgac ctctaccctg aatatactgt agcaggaaga
      181 aaagactggt ttgattttta tggttatgtt gatctaccaa aattctttgg cgtcggcagt
      241 cactatgatg ttgggatctg ggatgagggc tcaccactat ttacggaaat agaacctcgg
      301 ttttccattg acaaattgac cggattaaat cttgcgttcg gcccttttaa agaatggttc
      361 attgcaaaca actatgtcta tgatatgggt gacaaccagt catcccggca aagtacatgg
      421 tatatggggc ttggtacaga tatcgacacg ggtctaccaa ttaagctttc tgccaatata
      481 tacgccaagt atcagtggca aaactatggt gccgctaatg aaaatgaatg ggacggatat
      541 cgattcaaaa taaaatatag cattcctctt acccatttat tcggagggcg attggtatac
      601 aatagtttta ctaactttga ttttggctcc gatcttgcgg acaaatcaca caataataaa
      661 cgaaccagta atgctattgc ttcaagccat atcctttccc tgctatatga acactggaaa
      721 tttgcattta cactacgtta ttttcacaac ggtggacaat ggaatgctgg ggagaaagtt
      781 aacttcggag atggtccatt tgaattaaaa aacacaggat ggggaaccta tactactatt
      841 ggttatcaat tttaaatcag gtgatgtttt agatattttt acttaatgcc attttgtaat
      901 tgtcataaat aacttcacca ttcacactgt gaattcatcg ctcaacacaa aagccccctt
      961 attaccgggg cactgaccaa aggcaaaatg acaatcaatc accacctccg caggaggtgg
     1021 tttagaggtg acaacaaaag ctggcaactg ccagcttttg ttcaataatc actacaacaa
     1081 ctgcgccaga cggttaatat ccgactgaat cgccccggcg gtgacgtcgc gccctgcacc
     1141 tggcccgcgg atcaccaacg gattatcgcg ataccagcgg ctttcgatgg caaagacgtt
     1201 atcgcacggt agtagtgatg ccagcggatg gtcttctcgc accgcttcca cgcctactcg
     1261 cgctttgccg ttggcatcga aacgcgccac gtagcgcaac accagcccca tttcacgggc
     1321 cgcttccaac cgttgtacca tctgctcatt aagttcatcg ccattttcaa agaaatggtc
     1381 gatgcttcca ccttcgcaat gggttggcac cagtgattca acacgcacct ggtccggttc
     1441 aatgttgtaa cccgcttcac gcgccagaat caccagcttg cgcatcacat ctttgccgga
     1501 gaggtcatca cgcgggtcag gttcagttaa gccctgctgc cacgcctgat ccaccagctc
     1561 agtaaacggc acgctaccgt cgaattgcag gaacagccag gagagcgtgc cggagaagat
     1621 cccgctgatc gacaaaatag tgtcgccgct gtcgatcaga tcgcgcacgg tatggttgat
     1681 cggcaaacct gcgccgacgg tggcgttgta cagccagtga cgcccggttt tttcgaacgc
     1741 gtcgtggatc tggcgatatt tattgctgtc gctcgctccc gccagtttgt tggcactgat
     1801 gacgtggaaa ccgtggctgg cgaaatcgag atactgatca gcaagctgct ggctggcagt
     1861 aacgtccagc acaactaaat catcatacgg atgggcacgc atccacagga atagcgactc
     1921 ttcatcctgt tcgacggctt catcattaaa gaaggctagc gcacggctgg cgtccagccc
     1981 gtcatagctc aacaggctgc gacggctgtc caccacgcct gccagcacaa attcaaagcc
     2041 ggtgcgcgcc gagagcgtgc tctgctcacg ggcgaacagt tccagccagc gggaaccgat
     2101 atttccctta ccgaacaata ccaggccgat gcgtttttct gcgcggaaga cggactgatg
     2161 cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt actgccacca ggctgatgcc
     2221 gtcatcggac tgccaggtaa actcgaccgg ctggcctttc agttgctgcc agaagcggtg
     2281 gcaatgcagc gggttacggg tgacgcccgc cccaaccatc gccaccagcg ccagcccctg
     2341 acgcagacgc agttcgccgg gtaatcctgc ttcgtcgagg attttcagcg cactgtcggc
     2401 cacttccgag gtgtagcaaa attgcagcaa ctggcgatcg ttatgcacgc caactgccag
     2461 tgggcgcacc tgagcacgtt tcaggatttg gtcgatctct ttatgcgcca gtttaaaatc
     2521 ctgactggcg ggcacctgaa actcaatcaa acagacatca tcgtggctgg tgacaatacg
     2581 cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg gaaccttgat ccggcgtgta
     2641 gctacagcgc agctgcaggt cgatttcact accggatact ggctgtaaag tacgggcgtg
     2701 aagaacgggg gccgccagac gcgccagttc gctggcttca tcaagacgca gcaatggcag
     2761 cagacaggca tctttcactt tacgcgggtc ggcactatat accccggcga catcgctcca
     2821 gatggttacg cgggaaacac ccgccagcgc accgatttgt gtcgcggaat agtcggaacc
     2881 gttacgcccc agcagcaccg tttcaccggc gttgttacgg ctgataaacc cggtcaccac
     2941 cagacgtttg cccggatgtt gtaccagtag ctgttgcagc aacgggtaag aaagcccttc
     3001 atcaacctgc ggctgcgcgg cgcgttcagc gcgtaaaaac tcgcgggcat caagccaggc
     3061 cgctggcagc ccttgttgat taagtaccgc agacatcagg cgcgccgacc acacttcacc
     3121 gtggcccacc acttccgcat acaccgcatc attaataccg ctgtcgagca gcgccgcgag
     3181 gcgttccaga tcgctgacaa aggcgctaat gaggctatcg gcttcttcgg cgggtagcag
     3241 gccgctaatc agatcgcact gataacgacg tagcgtttgt tgaacctgat gcgcagagag
     3301 acgatcggtc tggcttagtt tcaaccagtt aatcaactgg ttagtggtgc taccggcggc
     3361 ggaaaccacc atcatatcgt caggctgaga gtactccgcc ataatgcccg cgacacgcaa
     3421 ataacacttc acatcagcca gactactgcc accaaattta tgcagttgac gacctttcgc
     3481 ccctgcctgc gcaatcacac tcatttttac cccttgtttg cagcccggaa gccattttcc
     3541 aggtcggcaa ttaaatcttc gccatcttca ataccggtgg agatacgtag cagcgtctcg
     3601 gagatcccgg cggcagcacg cgcttctggc gccatgcctg catgtgtcat ggttgcggcg
     3661 tgagagatta aactttccac tccccctaat gattccgcca gcgtaaacaa cgacaacccg
     3721 cccaggaaac gacgcagcgt ctgctcatcg ccatccagtt caaaactcaa cattgcgcca
     3781 aagccttttt gctggcgcgc ggcaatttca tgcccctgat tttccggcag cgacgggtga
     3841 tacagttttt tcaccaacgg ctgggtttgc agatatttca caatcgcctg cgcgttgcgc
     3901 tgcgccagct ccatacgcgg caccagcgtt cgcaacccgc gcagcagcag atagctgtca
     3961 aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg caagttcagt gacaacgtcc
     4021 gggtctttag caatcactac gccggccacc acgtctgagt gaccgttcag atatttcgta
     4081 catgaatgca acaccagatc ggcacctaat gccagcggat tttgtaatgc cgggcttaag
     4141 aaggtgttat ccaccacgct caccgccccg acttcccttg ccagatggca gattttcgca
     4201 atatccacga cgcgtaacaa tggattactt gggctttcta ccagtaccag tttgggtttt
     4261 tctgccagcg ctacccgtaa tgcctgttca tcgccttgat caacaaacaa cacgcgatag
     4321 caaccgcgtt tcgccagact gtcgaacagg cgatagctgc cgccatagca gtcgtgcggc
     4381 gcaaccagca gatcgcccgg tttcaaaaag acggtcgtta ccagatgaat cgcggacatg
     4441 ccggtattag taagtacggc accagcaccg ccttccagtt ctgccagcgc acgctgaacc
     4501 acatcgcgcg ttgggttgcc gcgccgcgag taatcatgcg cgcgcggttc attaaatccg
     4561 gtaaagttat aggtgctgga aagatggatc ggtgggacaa cgcaaccata ctgttcgtcg
     4621 tcatttaacc cgctacgcac tgcgatggtg gcctgtttac gcgtcatgtg atgaagttcc
     4681 ctgggcttgg tcggtgaaat gtcaggcacc agagtaaaca ttgtgttaat ggacgtcaat
     4741 acatctggac atctaaactt ctttgcgtat agattgagca aatcccaaat agccgttaaa
     4801 attatatgca ttatcacgcc gacaggtgca ttacacgatg tcacggtagc gcctgtacgg
     4861 taaactatgc gggtttacgg tcagtaccca cataaattgt gtggtctggt ctcaatttat
     4921 tgacgaagag gattaagtat ctcatggctg aatggagcgg cgaatatatc agcccatacg
     4981 ctgagcacgg caagaagagt gaacaagtca aaaagattac ggtttccatt cctcttaagg
     5041 tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt gaacaacctg cgtcacgcta
     5101 ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt taccgggcaa cctttgccgg
     5161 atgatgccga cctgcgtaaa gagcgcagcg acgaaatccc ggaagcggca aaagagatca
     5221 tgcgtgagat ggggattaac ccggagacgt gggaatacta aagcggaaaa gagcggcgcg
     5281 gagttaacca gccgcttttt tcatacatgg acgtttaacg atgaaaatca ggctactgat
     5341 cctcagcctg ctggtttctg tgcctgcctt tgcctggcag ccgcaaactg gcgacatcat
     5401 ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa ctggcgaccc attccgatta
     5461 cagccacacc ggtatgctgg tgatacgcaa caaaaagccc tacgtttttg aagcggtcgg
     5521 cccggtaaaa tacaccccac tcaagcagtg gatcgcccat ggtgaaaagg gcaaatacgt
     5581 tgttcgccgc gttgaaggcg aactgagtgt tgaacaacag caaaaactgg cgcaaacggc
     5641 aaaacgttat ctcggtaaac cgtatgactc cagcttctcg tggagcgacg atcgccagta
     5701 ctgttcggaa gtggtttgga aggtttatca gaacgcgctg ggaatgcgcg tgggcaaaca
     5761 gcaaaagttg aaagagttcg accttagtaa cccgcaggtt caggcaaagc tcaaagaacg
     5821 ctacggcaaa aatattccgc ttgaggaaac ggtggtctcg ccacaagccg ttttcgatgc
     5881 gccacaactc accacggtcg ccaaagaatg gccgctgttt tcgtggtaat acgaccaaaa
     5941 ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc
     6001 ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct
     6061 gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa
     6121 cggtggagcg gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt
     6181 tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc
     6241 cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtatga
     6301 aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg
     6361 caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc gttgcccacg
     6421 ttgccttgcc cgttccgctt ccccgtacct ttgactatct gctgccagaa ggcatgacgg
     6481 tcaaagccgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc atcggggttg
     6541 tggtgtcagt tagcaatgtc agcgagctgc cgcttaacga gctgaaagcg gtagttgaag
     6601 tgctggacgt tgagccggta tttactcact ccgtctggcg attactgctc tgggcggcag
     6661 attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactacgcc
     6721 aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggtcagg
     6781 cggtggatct gaacagcctg aaacgctcac ccaagcaaca acaggcgctg gcagcgttac
     6841 ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga atttaatgat gccgcgttgc
     6901 aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagtgact
     6961 ggcgaaagaa ttatgccgtt tctggtgagc ggttgcgatt gaatactgaa caggccaccg
     7021 ccgttggcgc aattcatagt gcggcggata ccttctctgc ctggctgctg gcgggcgtta
     7081 ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc gctcagggca
     7141 aacaggcgct ggtgatggtg ccggaaatcg gcctgacgcc gcaaactatc gcccgttttc
     7201 gtgaacgttt taatgcgcca gtggaagttc tgcattccgg cctgaacgac agcgagcgtc
     7261 tttcggcgtg gttgaaagcg aaaaatggtg aggcggcgat tgtgatcggc acccgctccg
     7321 cgctgtttac gccgtttaaa aatctcggtg tgattgtcat tgatgaagag cacgacagtt
     7381 cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtt tatcgtgcgc
     7441 acagcgagca aatcccgatt attcttggct ccgcaacacc cgcactggaa acgttatgca
     7501 acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg tcgggcgggg aatgcgcgtc
     7561 cggcaattca acacgtgctg gatttaaaag gtcagaaggt gcaggcgggg ctggctccgg
     7621 cgttaatcac ccgtatgcgc cagcatttac aggccgacaa ccaggttatc ctctttctta
     7681 accgccgtgg ctttgcgcct gccttgctgt gccacgactg cggctggatt gccgaatgtc
     7741 cacgttgcga tcactactac acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact
     7801 gtgacagcca acgcccggtg ccgcgccagt gcccttcctg cggttccacg cacctggtgc
     7861 cggtggggct gggcaccgaa cagcttgaac agacgcttgc gccgctgttc cctggtgtgc
     7921 ccatttctcg tatcgaccgc gataccacca gccgcaaagg agcgctggaa caacaactgg
     7981 cggaagtgca tcgcggcggc gcgaggatct tgatcggcac gcaaatgctg gcgaaaggtc
     8041 accactttcc ggatgtgacg ctggtggcgt tactggacgt agacggcgcg ctgttttctg
     8101 ccgattttcg ctcggcagag cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg
     8161 ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca tcatccagaa cacccgctat
     8221 tgcaaacgtt gctctataaa ggctatgacg cctttgccga acaggcactg gctgagcgac
     8281 gcatgatgca gctgccgccg tggaccagcc atgtgattgt gcgtgcggaa gatcataaca
     8341 atcagcacgc gccgctgttc ctgcaacaac tgcgtaatct catcctctcc agcccgctgg
     8401 cagacgacaa actgtgggtt ctcggtcccg ttccggcact ggcacctaaa cgtggcggcc
     8461 gctggcgctg gcaaattttg ttgcagcacc cttcccgcgt gcgattacag cacatcatca
     8521 atggtacgct ggcgcttatt aatacaatac cggattcccg taaggtgaaa tgggtgctgg
     8581 atgtcgatcc catcgagggc taatgcgctc acgatgcgag gcggatcgaa aaattcaata
     8641 ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt
     8701 tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt
     8761 gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt
     8821 ctctacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg
     8881 taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcagccta tggggcgcaa
     8941 cgtcaagcgt aatgaatccc gcactattct ggtgattgtc ccggatatct gcgatccctt
     9001 ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct
     9061 gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac
     9121 caagcaaatt gatggcatgt tgctgctggg ttcaaggctg ccgtttgatg ccagcattga
     9181 ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg aactggagct
     9241 gcctaccgtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt atttatatga
     9301 gcaagggcat aatcggattg gctgtatagc cggtcccgaa gagatgccgc tgtgtcacta
     9361 ccgcctgcaa ggctatgtcc aggcgctgcg tcgctgcggc attatggttg atccgcaata
     9421 catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc agttgcttga
     9481 tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact
     9541 ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga
     9601 taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta
     9661 cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg
     9721 cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc
     9781 ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg
     9841 cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc
     9901 gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc
     9961 tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg
    10021 gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacactg caaagccaga
    10081 aagtgaccgg aaacggacta ccaccaaaac cagaagaacg ctggcgctac atcaaagagc
    10141 tggaaagtcg ccagccgggt gtgcgtgcgc ctacagaacc ttctgccggt ggtgaagtga
    10201 aaacgccgga gcaactgaca ccagaacaac gccagcttct tgagcaaatg caggctgata
    10261 tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc
    10321 gtcagcaaac gctacagcgc cagcgtcagg cgcagcaatt agcggagcag caacgcctgg
    10381 ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg
    10441 cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc tactcagcag ccgtaccagg
    10501 atctgctgca aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa gctgcgccgg
    10561 ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga
    10621 tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt gctcagctgg
    10681 cgttcgaagg ctttgactca aaaattacca ccaacaatgg ctggaatcgt gtggtgattg
    10741 gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcgcttgaag atagcgggtc
    10801 atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat
    10861 aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg
    10921 tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga
    10981 tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct
    11041 gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt
    11101 cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga
    11161 gctggcgaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcat tgctggcagt
    11221 cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa
    11281 cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc gcgcgctgtt
    11341 agaaaacact gaacttagcg cccgtgaaat tgctgaaaag gcgttggata ttgcaggcga
    11401 catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg
    11461 atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat
    11521 cggccaggac aacgccaagc gttccgtggc gattgctctg cgtaaccgct ggcgtcgcat
    11581 gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc
    11641 gaccggtgtc ggtaaaactg aaatcgcccg tcgcctggct aagctggcga atgcgccgtt
    11701 catcaaagtt gaagcgacca aattcaccga agtgggctat gtcggtaagg aagtggattc
    11761 tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa
    11821 aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtac tgatcccacc
    11881 ggctaaaaat aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc
    11941 attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc
    12001 cgcagcaccg atgggcgttg aaattatggc tcctccgggc atggaagaga tgaccagcca
    12061 gctgcagtcc atgttccaga acctgggcgg tcagaaacaa aaagcgcgta agctgaaaat
    12121 caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga
    12181 gctgaagcaa gatgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat
    12241 cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggcgttca
    12301 gcgtgacctg ctgccgctgg tagaaggttg cactgtttcc accaaacatg ggatggtcaa
    12361 aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct
    12421 gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgag ctgcaggcac tgaccaccag
    12481 cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt acaaagcact
    12541 gatggcaact gaaggcgtaa atatcgagtt taccgactcc ggtattaagc gcatcgcgga
    12601 agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacataccgt
    12661 tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaactat
    12721 cactattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct
    12781 gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa
    12841 ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg
    12901 gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac
    12961 ggcgctggca tggtggcaag gtcacttcga tccactggtc gccctgctgg cattgattac
    13021 cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg
    13081 cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca
    13141 gcaagagatg aaacgggcgc tcattattac cgttgtgctc atctgtcttt ccgggctggc
    13201 actggttgca gtggcgtgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg
    13261 gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg
    13321 tttgggtgat atttccgtac tggttttctt tggctggttg agcgtcatgg ggagctggta
    13381 tttacaggct catacattga ttccggcact gatccttccg gcaaccgcat gcggcctgct
    13441 ggcaacggca gtactgaaca ttaataacct gcgtgatatc aatagcgacc gcgaaaacgg
    13501 caaaaacacg ctggtggtgc gcttaggtgc agtaaacgcg cgtcgttatc atgcctgcct
    13561 gctgatgggc tcactggtgt gtctggcgct gtttaatctc ttttcgctgc atagcctgtg
    13621 gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt atgtgatgcg
    13681 ggaaatggac ccggtggcga tgcgaccgat gctggaacgc actgtcaagg gagcgttact
    13741 gactaacctg ctgtttgttt tagggatatt cctaagccag tgggcagcat aactgacaaa
    13801 tatcaattaa caattgatga ttttgccaac agcctacata gcgcgatata ctgaaaattc
    13861 tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat
    13921 caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt
    13981 ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc
    14041 gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca
    14101 ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggtcatt
    14161 tacggcgcgg tgcgtcaggt agatgacctg gaagagctgg atatcggcat ccaggcaatg
    14221 gccgcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat
    14281 tttggcggtg taaccttctt ctccggcgac catctttacg ccgacaatac cgggattatt
    14341 ctttcagaag atccgctgga tattgaatga taaaaaaggc accgcaaggt gccttttttc
    14401 tgcgttacct gttggcctac acagtaaaga aattacgcga aagatgaagc gtaatcagac
    14461 ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt
    14521 cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg
    14581 cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt
    14641 atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat
    14701 acctctcctg aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat taccggtgac
    14761 gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg cgccatcgct
    14821 gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc tcctggagac
    14881 ataatccaca ccaatcgaaa atgttaataa atttgttgcg cgaatgatct aacaaacatg
    14941 catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg acgaagcaca
    15001 cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta cagcatgcct
    15061 acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact cttcaggatc
    15121 cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc ctcggtaccg
    15181 ggttgttgat ttttttcggt gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt
    15241 ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc atctacctga
    15301 ccgcaggggt ttccggcgcg catcttaacc ccgctgttac cattgcattg tggctgtttg
    15361 cctgtttcga caagcgcaaa gttatccctt ttatcgtttc acaagttgcc ggcgctttct
    15421 gtgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc gagcagactc
    15481 atcatattgt tcgcggcagt gttgaaagtg ttgatctggc tggcactttc tctacttacc
    15541 ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt accgctattc
    15601 tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc ggccctttgg
    15661 ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc ccattgacgg
    15721 gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct
    15781 ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg ccgctttttg
    15841 gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt
    15901 tgccttgcga tatctgtgtt gtggaagaaa aggagaccac aactccttca gaacaaaaag
    15961 cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaaata tatcgttgcg
    16021 ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt
    16081 agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac caggctgggt agaacacgac
    16141 ccaatggaaa tctgggccac ccaaagctcc acgctggtag aagtgctggc gaaagccgat
    16201 atcagttccg atcaaattgc agctatcggt attacgaacc agcgtgaaac cactattgtc
    16261 tgggaaaaag aaaccggcaa gcctatctat aacgccattg tctggcagtg ccgtcgtacc
    16321 gcagaaatct gcgagcattt aaaacgtgac ggtttagaag attatatccg cagcaatacc
    16381 ggtctggtga ttgacccgta cttctctggc accaaagtga agtggatcct cgaccatgtg
    16441 gaaggctctc gcgagcgtgc gcgtcgtggt gaattgctgt ttggtacggt tgatacgtgg
    16501 cttatctgga aaatgactca gggccgtgtc catgtgaccg attacaccaa cgcctctcgt
    16561 accatgttgt tcaacatcca taccctggac tgggacgaca aaatgctgga agtgctggat
    16621 attccgcgcg agatgctgcc agaagtgcgt cgttcttccg aagtgtacgg tcagactaac
    16681 attggcggca aaggcggcac gcgtattcca atctccggga tcgccggtga ccagcaggcg
    16741 gcgctgtttg gtcagttgtg cgtgaaagaa ggaatggcga agaacaccta tggcactggc
    16801 tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc
    16861 accatcgcct gcggcccgac tggcgaagta aactatgcgc tggaaggtgc ggtgtttatg
    16921 gcgggcgcgt ccattcagtg gctgcgcgat gagatgaagt tgattaacga cgcctacgat
    16981 tccgaatatt tcgccaccaa agtgcaaaac accaatggtg tgtatgtggt tccggccttt
    17041 accgggctgg gtgcgccata ctgggatccg tatgcacgcg gggcgatttt cggtctgact
    17101 cgtggggtga acgctaacca cattatacgc gcgacgctgg agtctattgc ttatcagacg
    17161 cgtgacgtgc tggaagcaat gcaggccgac tccggtattc gtctgcacgc gctgcgcgtg
    17221 gacggcggtg cggtggcgaa caatttcctg atgcagttcc agtccgatat tctcggtacc
    17281 cgcgttgagc gcccggaagt gcgcgaagtc accgctttgg gtgcggccta tcttgctggt
    17341 ctggcggttg gcttctggca gaatcttgac gagctgcaag agaaagcggt gattgaacgc
    17401 gagttccgtc caggcatcga aaccactgag cgtaattacc gttacgcagg ctggaaaaaa
    17461 gcggtcaaac gcgcgatggc atgggaagaa cacgacgagt aataatgatt gcctgatgcg
    17521 acgcttgcgc atcttatcag gcctacgagt cggatacaca tccggcatca cgcagactct
    17581 gaaaccgtgc gggggcaacc ccgcacacat caataatccc gcccttcccc tgtgctacac
    17641 ttcgcgccat tccttactgc ttagagtttg ctatgagacg agaacttgcc atcgaatttt
    17701 cccgcgtcac cgaatcagcg gcgctggctg gctacaaatg gttaggacgc ggcgataaaa
    17761 acaccgcgga cggcgcggcg gtaaacgcca tgcgtattat gctcaaccag gtcaacattg
    17821 acggcaccat cgtcattggt gaaggtgaaa tcgacgaagc accgatgctc tacattggtg
    17881 aaaaagtcgg tactggtcgc ggcgacgcgg tagatattgc tgttgatccg attgaaggca
    17941 cgcgcatgac ggcgatgggc caggctaacg cgctggcggt gctggcagta ggcgataaag
    18001 gctgtttcct caacgcgccg gatatgtata tggagaagct gatcgtcgga ccgggagcca
    18061 aaggcaccat tgatctgaac ctgccgctgg cggataacct gcgcaatgtt gcggcggcgc
    18121 tcggcaaacc gttgagcgaa ctgacggtaa cgattctggc aaaaccgcgc cacgatgccg
    18181 ttatcgcaga aatgcagcaa ctcggcgtgc gcgtatttgc tattccggac ggcgacgttg
    18241 cagcctcaat tctcacctgt atgccagaca gcgaagttga cgtgctgtac ggtattggtg
    18301 gcgcgccgga aggcgtggtt tctgcggcgg tgatccgcgc attagatggt gacatgaacg
    18361 gtcgtctgct ggcgcgtcat gacgtcaaag gcgacaacga agagaatcgt cgcattggcg
    18421 agcaggagct ggcacgctgc aaagcgatgg gcatcgaagc cggtaaagta ttgcgtttgg
    18481 gcgatatggc gcgcagcgac aacgtcatct tttctgccac cggcatcacc aaaggtgatc
    18541 tgctggaagg tattagccgc aaaggcaata tcgccaccac cgaaacgctg ctgatccgtg
    18601 gtaaatcacg caccattcgt cgcattcagt ccatccacta tctggatcgt aaagacccgg
    18661 aaatgcaggt gcacatcctc tgattgattt gatcgattga gccttccagt ccttcgggac
    18721 tggaattttt ttgttcggag aacgaagata aggcaagtca atcaaaacag gagaaaaaca
    18781 tggctgattg ggtaacaggc aaagtcacta aagtgcagaa ctggaccgac gccctgttta
    18841 gtctcaccgt tcacgctccc gtgcttccgt ttaccgccgg gcaattcacc aaacttggcc
    18901 ttgaaattga aggcgaacgc gttcagcgcg cctactccta tgttaactcg cccgataatc
    18961 cggatctgga gttttacctg gtcaccgtcc ccgatggcaa attaagtcca cgactggcgg
    19021 cactgaaacc gggcgatgaa gtgcaggtgg ttagcgaagc cgctggcttc tttgtgctgg
    19081 atgaagtacc agattgcgaa acgctatgga tgctggcaac cggtacagcg attggccctt
    19141 atttatcgat tctgcaattg ggcaaagatt tagaacgctt caaaaacatg gtgctggttc
    19201 acgccgcacg atatgccgcc gacttaagct atctacctct gatgcaggaa ctggaaaaac
    19261 gctacgaggg gaaactgcgc attcagacgg tggtcagtcg ggaaacggct gcgggttcgc
    19321 tcactgggcg attaccggcg ttaattgaaa gtggtgaact ggaaagcgcg gttggcctgc
    19381 cgatgaataa agaaaccagc catgtgatgc tgtgcggcaa tccacagatg gtgcgcgata
    19441 cacaacagtt gctgaaggag acccggcaga tgacgaaaca tttacgtcgc cgaccgggcc
    19501 atatgacagc ggagcattac tggtaagcgg ttacttgtcg ataaacggca cgatcaagag
    19561 atccgcactc aacttattga tcatcccgcg atatgccggc atcaaacggt tgataaatga
    19621 gtgatgatga ccacagacaa ggaggtcgca ctgctctttt tgcataattt ccagcagcgt
    19681 ttccggcatt tctccgcgtt caatacgcag ttttgtcttc ggccattgaa tatttttcgt
    19741 cagtttatac agcttgttat ccgacttatt cttcaacaat tgaagaatat cttctgttgc
    19801 agggaagtag atacccggat acaactcgct taagccatca tcaatatgaa ttaacgtcag
    19861 gtgagcgtca ttatgtctgg cgagctccag ggctttattc accagtaagg catcttcttc
    19921 attcccggaa attgccacgc caatgtgttt ataagccatg tttaactcct tctaaagcaa
    19981 ctccatcaag ctaacgaacg cagtgataac tcaaaaataa tcatctcggc ctggcatgag
    20041 aaggtgaagg ccgcatcaag ctcaacttgc ccctccagat ccttaatctt ataggccacg
    20101 ctggcccctt catctgcaga ggatgcagcg gcggcaagct ctttcagttt ggtcataaaa
    20161 gactctgctt cttcgcgggt ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta
    20221 tcaatcaccg taccgatatc aatcgcacat cctttagtac tgcatttatc tacgacatct
    20281 ttcatagggc acctctgtat attcgcctct ctgtcgatta cgacttaaga ataatgcgcc
    20341 gttaaaggaa gaacaaacaa gtaatgatct tatggattac catttttttt gatattgctt
    20401 aaggtgaaga gttcagaagg attttccggg cattgt
//
LOCUS       DAZGAP010000092        20427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGAP010000092 DAZGAP010000000
VERSION     DAZGAP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.092"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..295)
                     /locus_tag="ACHLAM_002873"
     CDS             complement(<1..295)
                     /locus_tag="ACHLAM_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854374.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVVNAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPVGLSASDWKVHRGDLMVEGNIESNQKLIVLGNLTVK"
     gene            complement(359..1219)
                     /gene="pdxI"
                     /locus_tag="ACHLAM_002874"
     CDS             complement(359..1219)
                     /gene="pdxI"
                     /locus_tag="ACHLAM_002874"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO7854375.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(1450..2040)
                     /gene="paaY"
                     /locus_tag="ACHLAM_002875"
     CDS             complement(1450..2040)
                     /gene="paaY"
                     /locus_tag="ACHLAM_002875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO7854376.1"
                     /translation="MPIYQIDGLTQFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIHRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(2022..2174)
                     /locus_tag="ACHLAM_002876"
                     /pseudo
     CDS             complement(2022..2174)
                     /locus_tag="ACHLAM_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
     gene            2175..2381
                     /locus_tag="ACHLAM_002877"
                     /pseudo
     CDS             2175..2381
                     /locus_tag="ACHLAM_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2553..2879)
                     /gene="ydbL"
                     /locus_tag="ACHLAM_002878"
     CDS             complement(2553..2879)
                     /gene="ydbL"
                     /locus_tag="ACHLAM_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO7854377.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(2887..3072)
                     /gene="ynbE"
                     /locus_tag="ACHLAM_002879"
     CDS             complement(2887..3072)
                     /gene="ynbE"
                     /locus_tag="ACHLAM_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO7854378.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(3069..5708)
                     /gene="ydbH"
                     /locus_tag="ACHLAM_002880"
     CDS             complement(3069..5708)
                     /gene="ydbH"
                     /locus_tag="ACHLAM_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO7854379.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGHLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
     gene            5916..6905
                     /gene="ldhA"
                     /locus_tag="ACHLAM_002881"
     CDS             5916..6905
                     /gene="ldhA"
                     /locus_tag="ACHLAM_002881"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7854380.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            7016..7438
                     /gene="hslJ"
                     /locus_tag="ACHLAM_002882"
     CDS             7016..7438
                     /gene="hslJ"
                     /locus_tag="ACHLAM_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO7854381.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(7435..7701)
                     /gene="ydbJ"
                     /locus_tag="ACHLAM_002883"
     CDS             complement(7435..7701)
                     /gene="ydbJ"
                     /locus_tag="ACHLAM_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO7854382.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTTIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            7975..11499
                     /gene="nifJ"
                     /locus_tag="ACHLAM_002884"
     CDS             7975..11499
                     /gene="nifJ"
                     /locus_tag="ACHLAM_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO7854383.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRTVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSVDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            11865..12998
                     /gene="ompN"
                     /locus_tag="ACHLAM_002885"
     CDS             11865..12998
                     /gene="ompN"
                     /locus_tag="ACHLAM_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO7854384.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            13139..13573
                     /gene="uspF"
                     /locus_tag="ACHLAM_002886"
     CDS             13139..13573
                     /gene="uspF"
                     /locus_tag="ACHLAM_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO7854385.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            13749..14684
                     /gene="ttcA"
                     /locus_tag="ACHLAM_002887"
     CDS             13749..14684
                     /gene="ttcA"
                     /locus_tag="ACHLAM_002887"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO7854386.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(14813..16186)
                     /gene="dbpA"
                     /locus_tag="ACHLAM_002888"
     CDS             complement(14813..16186)
                     /gene="dbpA"
                     /locus_tag="ACHLAM_002888"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO7854387.1"
                     /translation="MSAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPTILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRMLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(16216..16389)
                     /gene="ynaL"
                     /locus_tag="ACHLAM_002889"
     CDS             complement(16216..16389)
                     /gene="ynaL"
                     /locus_tag="ACHLAM_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO7854388.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(16664..17647)
                     /gene="zntB"
                     /locus_tag="ACHLAM_002890"
     CDS             complement(16664..17647)
                     /gene="zntB"
                     /locus_tag="ACHLAM_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO7854389.1"
                     /translation="MEAIKGADVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDFEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(17661..17708)
                     /gene="ydaN"
                     /locus_tag="ACHLAM_002891"
     CDS             complement(17661..17708)
                     /gene="ydaN"
                     /locus_tag="ACHLAM_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO7854390.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            17902..19134
                     /gene="dgcM"
                     /locus_tag="ACHLAM_002892"
     CDS             17902..19134
                     /gene="dgcM"
                     /locus_tag="ACHLAM_002892"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO7854391.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLEDGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(19155..19718)
                     /gene="smrA"
                     /locus_tag="ACHLAM_002893"
     CDS             complement(19155..19718)
                     /gene="smrA"
                     /locus_tag="ACHLAM_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO7854392.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(19913..>20427)
                     /locus_tag="ACHLAM_002894"
     CDS             complement(19913..>20427)
                     /locus_tag="ACHLAM_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854393.1"
                     /translation="GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVT
                     LTLGGIPKERLKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHT
                     VNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAF
                     LLVPAMNKSS"
BASE COUNT         5291 a         5253 c         4841 g         5042 t
ORIGIN      
        1 ctttgactgt caaattgcca agaacaatca atttttgatt actctctata ttaccttcta
       61 ccattaaatc gccacgatgc actttccagt ccgatgcaga taaaccgacg gggacaagat
      121 tcacgctact gttgatagct tgttcaactt gttgtttatc ttcagcggta gccgcagaaa
      181 aaaatggttc aatatcaact gcatttacaa cacatcgaag ttccgcctgt actggaagac
      241 tcagcataaa caggggaagt aaacctatcc agctgcaaat cctgggctta aacatatgca
      301 ctccatgcca ttaaaaaaga aacaaaaggg aaatgcatct ttcccttttg tatattcgtt
      361 attctcgcga aataccgtcc aacgtagaca acacttcctc agaaagatgc aatttttcag
      421 cagccatatt ctcccgtaaa tgcgcaacag aagacgtccc tgggatcagc aaaatattcg
      481 gtgaacgctg taacagccac gccagcgcca cctgcattgg tgttgcgccc aggctcgctg
      541 ctacatccga aagtgtggag gattgcagcg gcgtaaagcc cccaagcgga aagaacggca
      601 cgtaggcaat gccgtcacga gccaaagcat caatcattgc gtcatcagca cgatgagcaa
      661 tgttgtattc gttctgcaca cagacgattt cggtaatctt acgcgcctct gcaacctgcg
      721 tcggtgtaac gttgctcaga ccaatatgtt ttaccaggcc ttgttgttgc atctctgcca
      781 gcacggtcag gctggcttca atcgatcctt ccgctgggcc atgaccatcc cccatcataa
      841 cgcgcaggtt aaccacatcc agtgcgccca gcccgagatt acgtagatta tcgtgcaccg
      901 ctttttgcag ctctgccgga gaaaatgcgg gcaaccagga tgcgtcctct ccacgccgcg
      961 caccaatttt agtgacaatt gtcaggtcgt cagagtaagg ataaagcgct tcgcggataa
     1021 tctgattggt gacgtgcgga ccataaaagt cgctggtatc aatatgattg acgcccaatg
     1081 ccagcgcctc acgcagcacg gttatagcga cgtggcgatc tcttgggggg ccaaaaaccc
     1141 caggacctgc cagttgcatc gcgccataac caagacggtt aacggatttt gtaccgagag
     1201 taaatgtatt gctgctcatc gcaccctgcc ctgtaataaa acggattaag aaaaatagta
     1261 tagaaacgaa aagagataaa taatgcgaag gcgtaaacaa gattactgga tgagttgctc
     1321 tattgccctg aaagacacca aaacaaaacg cgtcaagtac cgcatccggc aataaaaaaa
     1381 ccgcaacctg tatcagcgtt gcggtttagg tatcacatgc taaaaattag aacgtccagc
     1441 atcaggacgt tactgtttcg gtcgcagatt ctcatcaaat accaggcgct gcctgccagg
     1501 ttcaacttcc cgcaatggct caacttgatg caacgtctgc ttactgcgtg tcaccagcac
     1561 ctgatactca tgcgtgccct gctttttcca tgccagttcc tgctcactaa gttcacgaat
     1621 cgcttttgcc gggctgccga caatcaggta attagcaggc atttctgctt tggctttcac
     1681 aaatgcggaa gcgccaacaa tgctgttctc gccaatcacc gcaccgtcca tcactaccgc
     1741 gttcataccc actaatgcat tgcggtggat aatgcagccg tgaaggatag cgctatgacc
     1801 aatatgccca tcttcttcca cgacagtatc ctgatcggga aaaccgtgca taacgcaatt
     1861 atcctgaatg ttcgcgccat ctttcaccac gatacgaccg aaatcgccac gcaggctggt
     1921 atttggccca acgtacacgc ccttgccgag aataacatcg ccgatcaata ccgctgtcgg
     1981 atggacaaaa ctctcttctg gcacaaactg agtcagaccg tctatctgat aaattggcat
     2041 aacgcctcct gttcaatatt caagccgcca aaacgttgaa aatacaggct tcccggcgca
     2101 ggcaattcac cgaccgaggt ttcacctttt tcactaacga acgctaaagc agcaggcgct
     2161 acgcgctgat aaagtttgcc gataaaggtt tggtagtcgc gcaatatatc cgtaaccgtc
     2221 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
     2281 ttggctttca ttggggattt tccgaccaga gtcatttttc aacggtattt aagcaacgct
     2341 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattcgc gttgcatgta
     2401 cagcaacgcg aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
     2461 tttgacagga atttttgaat attcacgttg tttccggaaa atagattaat cgtcttctcc
     2521 tgtagcgtta tcccgatatt gcggttttgc tattaaaatt ttcgcaccca tttaccgttt
     2581 atcccttgta catattcccc gggtttggcc cgttctacaa gcttttgccc ggcgagtttc
     2641 gcgatatcat ccaccgacac attattctgc tttgccagtt gttgatagct cgctttacgt
     2701 tcggcattga tgtcggttac taatttctca gtttcagcat ctgttttcag cgcaaccaga
     2761 taaccataaa atgtttcgcc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
     2821 attacattgc tgcttaccaa gccaacaaga aatgcacaaa gaagtaatgt tcttttcatc
     2881 atcacctcaa aaaagatcgc tacgggtctc aagcagctct tcgacatctt tgtctgcctt
     2941 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
     3001 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
     3061 taaaattttc attgtttatc ctcacactct tttccttgcg gacagtcatt tcccggtaga
     3121 tgcgcgttct gctccagcca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     3181 gtaaaaatat tctcttcatg atggtaatta agatttaccg taccgctttt accatcaacg
     3241 cgactggtac ctgtaatatt ggcctgcatg gttaataaac cgagattatc taaattaatt
     3301 tttgtcgatg aacggctaat ttccatatag cgcaaccagt taattgctga acccgcggtc
     3361 atattgtctt tcaccaccgc atctgcggtg tctttatcca gccgcaaggt cattggcccg
     3421 ctatttgcca gccagccgtc tttcactatc catttttcgt tgtttagcca taacggcaat
     3481 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     3541 ctggatgaga gattattcag ccgcaacagc gccgagtcat gttgcggcat gcgtaattgt
     3601 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caatggttcg
     3661 tcttcactcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     3721 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     3781 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     3841 gcactaccgc ctttcaacac accgtgccct cccgcgcgga atccttgttc aggcgcagct
     3901 gaaaatgcaa cctgagcata cagttcgcca tcgcgtaagt tcatcttcca gtcgggtggc
     3961 accagcggct ggaatacagt cagtgattgt ttaggccacc aggcgttacc gcgcagacga
     4021 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg caaatcgcct
     4081 ttaaagagga aatacgtcgg atcgcgccca tcgacactaa attttaaggt tgatggcggt
     4141 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctaaag
     4201 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     4261 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     4321 atcgtgctgt catgccactc accctttcct gcgacatccc aggtggcatt catcggtgta
     4381 aaactgcctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     4441 gccagtccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     4501 gcctgcaaat gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     4561 atttcatcga tgtccagcga atcgatgact cttcccttcg aacgtaataa cgcgcctggc
     4621 tcaaaggcca gcgttgggtc agtcagactt ccgcttagct gcgcaggtaa acgggcatat
     4681 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca aaggcagctg gctgttatcc
     4741 atacttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     4801 gtcagcacac tcagtcgtcc gctgaccaga gcgttctcga gccctgcctg ccagttgtcg
     4861 actttgacac ccagtcgtcc actcaaagga aaacctgcat acggccagct ccagcgacca
     4921 tcgcttacgg tcaattgttg gcgagtaatt tgccacggca gatcgagcaa cggatcgccg
     4981 ttatcccgtg ccaccacaat caattgcccg ctattttcct gccagtccag ctcggcatcc
     5041 accagtgaag gctcctgcgg caagtttaac gtcgcagtag catgaccact tacaggaagt
     5101 ccatccggca ctagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     5161 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     5221 ttaacttttt cgccctgata acgcagttgc tggatatcgg aggttaatgc gagagagagt
     5281 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     5341 ggcaacatgg cctgccactg cgcgagggtt tttggagcgg caggcgattg ctccgtctgc
     5401 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     5461 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     5521 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     5581 cgtgtcccga gcggtagcca gatgcccgcc agcgtgggaa cccacagccc gagcgtcatc
     5641 agcagtgtca acggcacaag aataatcagt aataatagcg cgagaacggc tttatattta
     5701 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     5761 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     5821 tttttcaata tcgccatagc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     5881 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     5941 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     6001 tttttgactt tctgctgacg gaaaaaaccg ctaaaaccgc caatggctgc gaagcggtat
     6061 gtattttcgt aaacgatgac ggcagccgcc cggtgctgga agagctgaaa aagcacggcg
     6121 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag
     6181 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaagccgtt gctgaacacg
     6241 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     6301 acgccaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     6361 ttatcggtac cggcaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     6421 gtctgctggc gttcgatccg tatccaagtg cggcggcgct ggaactcggt gtggagtatg
     6481 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     6541 cggaaaacta tcatctgttg aacgaagctg ccttcgaaca gatgaaaaat ggcgtgatga
     6601 tcgtcaatac cagtcgcggt gcattgattg attctcaggc agcaattgaa gcgctgaaaa
     6661 atcagaaaat tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg
     6721 aagataaatc caacgacgta attcaggatg acgtattccg tcgcctgtct gcctgccaca
     6781 acgtgctgtt taccgggcat caggcattcc tgacagcaga agcgctgacc agtatttctc
     6841 agactacgct gcaaaactta agcaatctgg aaaaaggcga aacctgcccg aacgaactgg
     6901 tttaatcttg ccgctcccct gcattccagg ggagctgatt cagataatcc ccaatgacct
     6961 ttcatcctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     7021 gaaagtagcc gcgctcgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     7081 aattgctgta accccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     7141 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa agatgatgat
     7201 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     7261 cgccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     7321 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
     7381 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
     7441 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
     7501 acacatccca atcgtcgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
     7561 tgcacaattt gcttctccac tactggacat cgacgctttt aaacctggcg ctacgtgcgc
     7621 cgcagtcgcc tgctgaacag gttcactact acacgccgac aacaataaag cggcacaccc
     7681 tacccaaaac gctgctcgca tcttttcttc ctctgatctt caagccaaac gacaccgcca
     7741 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgagcgttg ccccgcattt
     7801 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tacgctgatg
     7861 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
     7921 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
     7981 actattgacg gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc
     8041 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
     8101 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
     8161 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tctcaacatc gtttacgtca
     8221 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
     8281 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactctctat ttttggcgat
     8341 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     8401 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     8461 tttattcatt tctttgatgg tttccgcacg tcccacgaaa tcaataaaat tgtcccgctg
     8521 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     8581 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     8641 cagtctcgcg aagccaccaa cccatggtac aacgcagtct atgaccatgt tgaacaggcg
     8701 atgaatgatt tcgctgccgc gacaggtcgc cagtatcagc cgtttgagta ttacgggcat
     8761 ccacaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     8821 gttgatgaat tgctgacccg tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc
     8881 cccttctccg ccaaacactt actgcaagcg ttgccggact ctgttcggac agtggcggtg
     8941 ctggatcgca ccaaagagcc aggtgcgcaa gcggaacccc tgtacctgga tgtgatgacc
     9001 gcactggcag aagcctttaa taatggcgag cgtgaaaccc tgccccgtgt cattggtggg
     9061 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     9121 ctcaacgcgg ctaaaccgaa agcgcgcttt acggtaggta tttacgatga tgtgaccaat
     9181 ctgtcactgc cgttgccgga aaacaccctg ccaaactcgg cgaaacttga agccttgttt
     9241 tatggccttg gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt
     9301 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa ggcgggcggc
     9361 ctgacggttt ctcaccttcg cgtgagcgag cagccgattc gttccgctta tctgatttcc
     9421 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgaa
     9481 cgtttaaaac ctggcggcat tttcctgctc aacacgccgt acagcgtaga tgaagtatgg
     9541 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     9601 attaacgcgg cgaaaatcgc ccgcgaatgt ggtctggcgg cccgtattaa taccgtcatg
     9661 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     9721 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     9781 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcagcc ggttaatccg
     9841 cacagcgcca atcgaccgcc tgtggtttcc gatgccgctc ctgatttcgt gaaaaccgtt
     9901 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     9961 ggcacctggc cgatgggcac cacgcgctgg gaaaaacgca atatcgccga agagatcccc
    10021 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgctgcttg cccacattcg
    10081 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
    10141 tcgctggatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
    10201 gaagattgca ccggctgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
    10261 ccagagatta aagccatcaa tatgatgtcg cgcctggaac atgtcgaaga agagaaaatc
    10321 aattacgatt tcttcctcaa cctcccagaa atcgaccgta gcaaactgga acgtattgat
    10381 attcgtacat cacagctgat tacaccgctg tttgaatatt caggtgcttg ctccggttgt
    10441 ggcgagacgc cgtatattaa attgctgact cagctctatg gcgaccgaat gttgatcgcg
    10501 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtatactacc
    10561 gatgccaacg gtcgtggacc ggcatgggcg aactcgctgt ttgaagataa cgccgaattt
    10621 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgttgcg tctgctggat
    10681 caatttgccg ataaaatccc ggcggaatta ctaactgcgt tgaaatcaga cgccacgcca
    10741 gaggttcgtc gtgaacaggt tgcagcttta cgccagcaac tcaaagatgt tgccgaagca
    10801 catgaactgc tacgtgacgc agatgcactg gtagaaaaat caatctggct gattggtggt
    10861 gatggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
    10921 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
    10981 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
    11041 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
    11101 ggcgcgcagc tcaaccagac ggtgaaagcg attcaggaag cggaagcgta tccggggcca
    11161 tcgctgatca tcgcttatag cccctgtgaa gagcatggtt acgatctggc actcagtcac
    11221 gaccagatgc gccaactcac tgccaccggc ttctggccgc tatatcgctt cgatccacgt
    11281 cgagccgatg aaggcaaact gccgctggca ttggattcac gcccgccgtc agaagcactg
    11341 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
    11401 gcagaacagt tatggaaaga tgctgcagct gatttgcaaa aacgctatga cttcctggca
    11461 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgcactggat aaggattatc
    11521 caattctaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
    11581 agtagcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
    11641 cgcagaataa cgtatagcaa attaaaagta taatttttat tttgtatgta ttatcttcaa
    11701 tacattcacc agacttatta tcatttcaga agaattattt gattaaggtt ttacttaagg
    11761 cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgctt tcactcccca
    11821 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agcaaagtac
    11881 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
    11941 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
    12001 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
    12061 aaattaacga tcaactcact ggctacggtc aatgggaata caatattcag gcaaacaaca
    12121 ctgaatcttc aacaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
    12181 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
    12241 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
    12301 gtcgtgccaa cggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
    12361 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
    12421 ccaacaacgg acgtgatgtt cgccatgaaa acggtgacgg ctggggtctt tccacaacat
    12481 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
    12541 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
    12601 aatacgatgc taacaatatt tacctggcaa ccatgtattc agaaacgcgt aacatgaccc
    12661 cgtttggcga cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactgcac
    12721 agtaccagtt tgattttggt ctgcgtccgg cagtctcttt cctgatgtct aaaggccgtg
    12781 acctgcacgc tgccggtggt gcagacaacc cggcaggtgt tgatgataaa gatctggtta
    12841 aatacgccga tgttggcgcg acttactatt tcaacaaaaa tatgtccacc tacgttgact
    12901 ataaaataaa cctgttggat gaagatgaca gcttctacgc tgccaacggc atctctaccg
    12961 atgatattgt cgctttaggt ctggtttatc agttctaaat cctcctgccc gctgttatgg
    13021 cgggcttttt ctgcttattc ttcctccttt gatctaaatt aaaaatgtga actccgtcat
    13081 tacacaaaaa gtgtcatctg gcgttacact ttatgcggat actaaaacag gaggttttat
    13141 gaacagaacg attcttgtcc ctatcgatat ttccgattca gaattaactc aacgcgtgat
    13201 tagccacgtt gaggcagagg caaagattga tgatgcagag gttcatttcc tgacggtaat
    13261 accttcactg ccctactatg cctctctggg tttagcgtat tccgcagaat taccggcaat
    13321 ggatgacctg aaagcggaag ccaaatcgca actggaagag atcattaaaa aatttaaact
    13381 gccaaccgac agagtgcatg tccatgttga ggaaggctcg cccaaagacc gcattctgga
    13441 attggcgaag aagatccccg ctcatatgat catcattgct tcccatcgac cggatatcac
    13501 cacttatctg ctcggttcta acgccgcagc ggtagtgcgt cacgcagagt gctccgtgct
    13561 ggttgtgcgc tgacattaac gcccgcacat tgctgcgggc tttttgattc atttcgcaaa
    13621 tgtgctgaca ttttcccctc taatccgtac catacacgcc acagttttta tatcagattt
    13681 cttatgctgg gcgttccggc atgacgcata ctcttctgat gccatataac gaattgagtc
    13741 gcttttaaat gtcgcaaaat caagaaatta gtaagaaaga acaatacaac ctgaacaaat
    13801 tacaaaaacg tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag
    13861 aaggcgatcg catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaaa
    13921 ttctgcgcaa tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg
    13981 atcaaaagca accgggcttc ccggaacacg ttctgcccga gtatcttgaa acgctgggcg
    14041 ttgagtacaa gattgtggaa gagaacactt acggtatcgt gaaagagaag attccggagg
    14101 gcaaaaccac ttgctcactg tgttctcgtc ttcgtcgcgg tatcctttat cgcaccgcaa
    14161 cggaactggg ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcaaacgt
    14221 tgttcttaaa tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg
    14281 atgatggcaa acatattgtg attcgtccgc tggcctactg ccgcgagaaa gatattcagc
    14341 gctttgccga tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta
    14401 acctgcaacg tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc
    14461 gtatcgagac gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca
    14521 acctgttcga tttcaaaggt attacccacg gttctgaagt ggttaacggg ggtgatctgg
    14581 cgtttgatcg cgaagagatc cctctacaac cggcgggttg gcagcctgaa gaagatgaaa
    14641 atcagttgga tgagttacgg ctgaatgtgg ttgaagtgaa ataacccgga tagcgcccga
    14701 tgcgcaagct taccgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc
    14761 cggcaatccc gaagaagatg tttactcttg caccctgcga ttcaacattt cattatttta
    14821 ataaccgcac ccggcacgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt
    14881 tatgagcaac agcctgacgc accgcgacat agacatgcgc cggatgcacg gcgattttgc
    14941 caatatctgc gccatcaagc ccgatatctc ctgtcagcgc acctaataca tcacccgggc
    15001 gcattttggc ttttttcccg ccatcgatac ataacgttgc catttctgct tccagcggca
    15061 caatggaact attaactggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa
    15121 tgatattggc ccgctgtgct tcttccgggg cacagaaact gatcgccaga ccgctatttc
    15181 ctgcacgagc tgtacgaccg atgcgatgta catgaacttc agggtcccac gccagctcaa
    15241 agttcaccac cagctcaagc gattttatat ccagaccacg cgcagcaaca tcagtcgcga
    15301 ccagtacacg ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct
    15361 ccagatcgcc gtgtaacgac aatgcacttt gccctacttc attcagcgcg tcgcagacag
    15421 actggcaatc ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta
    15481 ataaccgttg caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa
    15541 taggtggcag agcatctgtt gagtcaattt caatcgctaa aggatcgcgt tgcactcttc
    15601 cgctgattgc ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg
    15661 ctggcgcaaa acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt
    15721 cggcctcatc catcaccagc gtattcaacg catccagtga taccgtgcct ttttgcagat
    15781 gatccagcat acggcccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac
    15841 gctgcatacc gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac
    15901 gcgccagccg acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca
    15961 ctaaagcctg ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag
    16021 ccgccgtttt gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg
    16081 tcggaagcgc ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg
    16141 tgagttgggc gggaggcaaa acattcaggg tagaaaaagc ggacacaatt tattctcgtg
    16201 gtcatcgacg caaagtcagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa
    16261 tttaatcggt tcttcgtcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc
    16321 gggcactgga acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag
    16381 tgtcgtcata tttccctccg gtcattgggt ggactcttaa agggtagacg ctgacaaata
    16441 acaggcaaaa aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca
    16501 gtaattcaaa aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat
    16561 tcacctgcaa gagagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact
    16621 ttgcggcgtt tttgtataca gacagccggg aaaattgctt ttgttacaac catttactac
    16681 gatgcaacca taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa
    16741 atccgaattg ccacccgccg cctgggatcc caccaaggtt gacgccaaat aaccctgtca
    16801 gaaaggtact gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgcgcta
    16861 aattttcctg catcacctga gcgatttcat cagccatcac gccagtccgt gctatacagg
    16921 cgtcgatttc gtcaaggccg cgcccaaggc gatcggcaat atcctgcata cgacggcgtt
    16981 ggtcatcgct catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg
    17041 gtgccatata gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg
    17101 gtggaatttg ctgatcaagg agattatctt caaagtcgat aattttatcg tgcagctgct
    17161 cgataaattc gctggaatga tcggtcaacg catcacacac atccaccagc catcccccgc
    17221 aatcggtcgg accggtgcct tcttccagat cgcttaccac atcgtccagc gccagcactt
    17281 tgcgttgtcg ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt
    17341 gatcggggcg ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtaccttcac
    17401 cgagacggct gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg
    17461 gaagcagcgg tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga
    17521 gccagcaggg gtgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc
    17581 taccatccag catccaggca aatactgcat ccgggacatt aacgtccgct cccttaatcg
    17641 cttccacagt gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta
    17701 cgcaacatct catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat
    17761 aaaaaaagca aaatgctttt ccatatacaa accgtgtgaa gtgttaaata gcgtctatca
    17821 ttatcagaat tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt
    17881 gataaaaata acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc
    17941 agtcctgtct ggatcgtttc gccctttgag gaacagttaa tttatgccaa cagcgcggcg
    18001 cgactgttga tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc
    18061 tcacaaaaag aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa
    18121 atcctcaccg ttcagcgcaa tgaagaggaa acagcattga gctgtcggct tgttttgcga
    18181 aagctgacag aagcagaacc ggtgattatt ttcgaaggta tcgaagcacc ggcaacgctg
    18241 ggtttaaaag ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc
    18301 ttttttctga ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc
    18361 gtcgatgcta acctcgccgc gctcaatttc tatggctata accatgaaac gatgtgccag
    18421 aaacatacct gggaaataaa tatgctcggg cgacgcgtca tgcctatcat gcatgaaatt
    18481 tcgcatttac ccggtggcca taagcctttg aattttgttc ataaactgga ggatggttcg
    18541 actcgtcatg tgcagaccta tgccggtcca attgaaattt atggcgacaa gctcatgtta
    18601 tgtattgtgc atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc
    18661 caccatgatg cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca
    18721 ggccaaatgc agcacctcgc catcgctcag gattacagct tgttgctcat cgacaccgat
    18781 cgttttaaac acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc
    18841 cttgcccgca ctctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc
    18901 gaagagtttg tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa
    18961 actatccgcg taagcgtggc aaaagtgagt atttcgggct taccacgctt tactgtcagc
    19021 attggtgtgg cgcaccacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat
    19081 gatgctttgt atcgagcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccacg
    19141 gatgcgtctc gtgatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc
    19201 tgcgctgttt tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca
    19261 gtgcaatatg cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca
    19321 atattggcat gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa
    19381 ccatccgcca gggcttgttg aatgaaactg aacaccattt tgcggcactc ttccaccggc
    19441 tggcgcaaaa gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc
    19501 accccatgtt gcaacccttc ccgccgaaac tccagcggct gagttagcgg gatgatgtcg
    19561 agaaatccgg tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt
    19621 tggttacgcg ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc
    19681 atggcgtcaa gaaacagcga tttgtcgtca aggttcatgt aacctcctat gcaccttcac
    19741 acgtcgtgat tcagacgggt taattgttca tacttactgc atcgttccct tcacagattg
    19801 aaacaatact ctacttcata cctgtgagta tctgcgcagg cggggcattt tatactagcc
    19861 tgttagcagt atcgaacagt ttagcccgag tttaattcag cacaggctcc gatcaggatg
    19921 atttattcat agcaggaacc aacaaaaatg cgataaataa cagaccactg attagcagaa
    19981 aggtatccga aaatgccata gtttgcgcct ccctcagcat caactgactc aataatttgg
    20041 ttgaagccag tatttccgat gtctggtcac ttccactgcg attaaaaaac aaagcacttc
    20101 tggaaatgaa gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa
    20161 aatgaaaatt agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc
    20221 caagattacg tgttaaatta aatacaccag aggctaactt cagccgttcc tttggaattc
    20281 cccccagcgt taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg
    20341 cctgtggaaa cagtaactcc tgccatcccc attcgtgggt tataggggta aaaagataca
    20401 ttgaaaacac aaatccaccc agccccg
//
LOCUS       DAZGAP010000093        20377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGAP010000093 DAZGAP010000000
VERSION     DAZGAP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.093"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..71
                     /locus_tag="ACHLAM_002895"
     CDS             <1..71
                     /locus_tag="ACHLAM_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854394.1"
                     /translation="FHIEILPAALRCRLPPDCPLLR"
     gene            complement(153..926)
                     /locus_tag="ACHLAM_002896"
     CDS             complement(153..926)
                     /locus_tag="ACHLAM_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7854395.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(1032..2072)
                     /gene="gatD"
                     /locus_tag="ACHLAM_002897"
     CDS             complement(1032..2072)
                     /gene="gatD"
                     /locus_tag="ACHLAM_002897"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7854396.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDVVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(2120..3475)
                     /locus_tag="ACHLAM_002898"
     CDS             complement(2120..3475)
                     /locus_tag="ACHLAM_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO7854397.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(3479..3763)
                     /gene="gatB"
                     /locus_tag="ACHLAM_002899"
     CDS             complement(3479..3763)
                     /gene="gatB"
                     /locus_tag="ACHLAM_002899"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO7854398.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(3794..4246)
                     /gene="gatA"
                     /locus_tag="ACHLAM_002900"
     CDS             complement(3794..4246)
                     /gene="gatA"
                     /locus_tag="ACHLAM_002900"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO7854399.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(4256..5518)
                     /gene="gatZ"
                     /locus_tag="ACHLAM_002901"
     CDS             complement(4256..5518)
                     /gene="gatZ"
                     /locus_tag="ACHLAM_002901"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO7854400.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(5547..6401)
                     /gene="gatY"
                     /locus_tag="ACHLAM_002902"
     CDS             complement(5547..6401)
                     /gene="gatY"
                     /locus_tag="ACHLAM_002902"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO7854401.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(6630..7682)
                     /gene="fbaB"
                     /locus_tag="ACHLAM_002903"
     CDS             complement(6630..7682)
                     /gene="fbaB"
                     /locus_tag="ACHLAM_002903"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO7854402.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            7939..9216
                     /gene="yegT"
                     /locus_tag="ACHLAM_002904"
     CDS             7939..9216
                     /gene="yegT"
                     /locus_tag="ACHLAM_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO7854403.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            9213..10217
                     /gene="yegU"
                     /locus_tag="ACHLAM_002905"
     CDS             9213..10217
                     /gene="yegU"
                     /locus_tag="ACHLAM_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO7854404.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAYFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            10214..11179
                     /gene="yegV"
                     /locus_tag="ACHLAM_002906"
     CDS             10214..11179
                     /gene="yegV"
                     /locus_tag="ACHLAM_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO7854405.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAATLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11153..11899)
                     /gene="yegW"
                     /locus_tag="ACHLAM_002907"
     CDS             complement(11153..11899)
                     /gene="yegW"
                     /locus_tag="ACHLAM_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7854406.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(11951..12769)
                     /gene="yegX"
                     /locus_tag="ACHLAM_002908"
     CDS             complement(11951..12769)
                     /gene="yegX"
                     /locus_tag="ACHLAM_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO7854407.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(12834..13634)
                     /gene="thiD"
                     /locus_tag="ACHLAM_002909"
     CDS             complement(12834..13634)
                     /gene="thiD"
                     /locus_tag="ACHLAM_002909"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO7854408.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWTDTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(13631..14419)
                     /gene="thiM"
                     /locus_tag="ACHLAM_002910"
     CDS             complement(13631..14419)
                     /gene="thiM"
                     /locus_tag="ACHLAM_002910"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO7854409.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(14454..14550)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14642..14914)
                     /gene="rcnR"
                     /locus_tag="ACHLAM_002911"
     CDS             complement(14642..14914)
                     /gene="rcnR"
                     /locus_tag="ACHLAM_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO7854410.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            15036..15860
                     /gene="rcnA"
                     /locus_tag="ACHLAM_002912"
     CDS             15036..15860
                     /gene="rcnA"
                     /locus_tag="ACHLAM_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO7854411.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGKRNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            16079..16417
                     /gene="rcnB"
                     /locus_tag="ACHLAM_002913"
     CDS             16079..16417
                     /gene="rcnB"
                     /locus_tag="ACHLAM_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO7854412.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPDMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(16499..17533)
                     /gene="yehA"
                     /locus_tag="ACHLAM_002914"
     CDS             complement(16499..17533)
                     /gene="yehA"
                     /locus_tag="ACHLAM_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO7854413.1"
                     /translation="MEIRIMLFIVMMMVMPVSYATCYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLINLESIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAEDNGVGFML
                     ESNSSPVTFLNITDNSKGYTNLKEIAAKSKITDTTVSIPVTASYYVYDKNKVKSGALQ
                     ATALINVKYD"
     gene            complement(17549..20029)
                     /gene="yehB"
                     /locus_tag="ACHLAM_002915"
     CDS             complement(17549..20029)
                     /gene="yehB"
                     /locus_tag="ACHLAM_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO7854414.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCAITFGKEIDESRNYICQ"
     gene            complement(20045..>20377)
                     /locus_tag="ACHLAM_002916"
     CDS             complement(20045..>20377)
                     /locus_tag="ACHLAM_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7854415.1"
                     /translation="SPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSG
                     NGLVIKNDSENWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDH
                     GNYISDKI"
BASE COUNT         5108 a         5264 c         4689 g         5316 t
ORIGIN      
        1 acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtccat
       61 tgttgcgtta atcaaactat tctctgttga accagagaga atccgtaatg ccggatgcgg
      121 tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc
      181 ttctgcaatg ccagtttatc cacctcagac acagcatcat cggtaataat aatgtcaatt
      241 ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga atcgagaacg
      301 gcaatcactt tattcgccgc tgtcaccatc gcaccgctaa cggaataacc ttcattaaag
      361 gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta
      421 atatcctgca atgaacgctc agcaatggaa ccatgcatcg agcgcgtttt atggcgcacc
      481 gtaccaccac aaaccaccaa agtgatgtct ttattttcgg aaagggcaaa cgcagccggg
      541 agactgttgg taatcaccgt gatatttttg gcggtcatta atccttcggc gataagcatt
      601 gtcgtacttc cgctgtcgag aataacggtc atcccttcgt ggatcatttt gactgccgcc
      661 tgcgctattc tgtttttcgg cgcgctggcg agctgaaatc gctctttaaa cccgacttcc
      721 tgggtctcgg tttcgctatt accggacatt attttcgccg caccgccatg aaagcgcgta
      781 accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca
      841 aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc
      901 ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta
      961 aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcct gcatcacgct
     1021 ggcccgcggt ttcagggaat gagcaacact ttgcccggca tggcattacg agcgatgtca
     1081 cgcaccgcct gggcgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt
     1141 aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg
     1201 gagtagttca tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt
     1261 gcagatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg ggcatgagga
     1321 ccggcaatct ctaccgccag ttcgacggtt tgcgggacgc cagccgtctc gaggataagc
     1381 tgattaaagc gcagctcgcg taaaacgccc tgtatttgcg gcgcgctcat ttcacggctg
     1441 ttaaatgttt gcatcgctcc gaaagatttt gccagtgcca gtttttctga gctaatgtcg
     1501 atagccgtca cactctttgc tcccagcgcg acagcgcact gaattgccag caggccaatg
     1561 gttccggcac caataataat aacgttttta ttctcacatc cttgcgctaa atggaacgca
     1621 tgcaggccaa cggtaatcgg ttcaataaaa gcgccatcct caataggcat atccgcaggt
     1681 agtgcaaaga cattgtttcg cttaacgaca atatattcgg caaatccacc atcacgccgc
     1741 gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca ctctggacaa
     1801 gtaaaacagg gtaataacgg cacacaggca accacatcgc cagggtgtaa atcatcaaca
     1861 ccggatccca ccgcatcaat atagccgcta aattcatgac ctaacgttat tggataataa
     1921 tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt
     1981 tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttctgc aacgcgcacg
     2041 ataccatcag tatcattcac cactgatttc ataaaaactc ctgattatta agggggataa
     2101 cctccccctc aggtaaaaat tattctgcga gaacggcttt ctcttgttta ataaagccac
     2161 gcgctctacg ccaggtcatg aaaataccgg taagataaat ggcaccgata ataatgaaac
     2221 cgggaatatt ttgcggggag aaaacctgaa tcagtaacca ggtaatggga gaaccgccct
     2281 gatccattga agccaccatg cccccggctt ttaacgcccc agcattagcc gccagttggg
     2341 tgtgcaaacc aatagtttgc gtcgcgatcc acagggtgat gctcataatg atgacacccg
     2401 agattaaggt gcggaacaga tttccacgat gcaccgcgac cgccatcgcc acaaagaagc
     2461 cgatggtggc aaggtcgcca aacggcagca cctgattacc cggcacacaa acagcaatta
     2521 aaatggtgag tggaataaaa atcaggcttg ccgataccac cgccgtatgt cccagcagca
     2581 acgccggatc aaggccaatc aggaactcct gaccgccgaa cttcgcctgt aaacgactac
     2641 gggcctgctt agcgatgggc gttaaaccat ccatgatggg tttaatcacc cgtggcatca
     2701 gcagcataac tgccgccgtt tttaccgcca gctgcaatac acctttgaca tcgtaacccg
     2761 cgaggatgcc gataatcagc cccatcacaa aacccacggt gaccggttcg ccaaaagggc
     2821 caaatttgcg ctgaatatca tcggcgctaa atttaattcg gttaacgcct gggattttct
     2881 cgatgatagc atcgaccagc accgcaatcg gccccatata cgccgacgta ccgtgcggaa
     2941 tagcaatgcc ttccagctca aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct
     3001 tataaacaaa cgccgcgtga attaccacac ccgccatccc tatcatccat gaaccggttg
     3061 ccagatgcag caacgcgccg gtgaaggtca tatgccagat attccagata tcaacattta
     3121 ccacccgcgt catacgggtc agtaacatcg ccacgttaac cagaatcgca atcggaatcg
     3181 ccaccagcgc aatttgcgac gcccaggtca tgggtgaaga gcccggccag ccgacatcga
     3241 ccacatgcag attcaggtcg aaattttccg ccatcgcttt cgccgccggg ccaatggaat
     3301 ccagcattaa gccaatcaca aggccaatac caacaaagcc aatcccgata tgcagacctg
     3361 ctttaaagca atcgcctgcc ttcatgccta atattttaga aaaaataatg atgacaatcg
     3421 gcagcatcac cgtagggccg aggtcgagaa tataacgcat gacttctgaa aacataggtc
     3481 acccctgtaa gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaacaa
     3541 aaggcatgcc gtgaactaac ggaatatcgc caaaacttcg atccaccctg gcagtggtgc
     3601 atatcaaatg cacgccatcc atataggttt ctatttcatt aacccgacac tggattaatt
     3661 caacaggaat attatgactc tgacacaact ctttaatttc ttccgccgcc atcgtagagg
     3721 tcgcaaccgc gcctccgcaa gcgacaataa tcttgcgttt cataaatagt cctttttatt
     3781 gttacagagg gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact
     3841 gggtttcagg aagagtgatt agtgtctcga cgatctcggg ctgttgtaac ttgccaaata
     3901 aacagcgtaa aagtttcaat tgctgctgcg gattttccac aattaacgca atcaccaacg
     3961 ataccgccac gtcgttatca tcatccgctt gctgaaaatg aactttattt gttggcctta
     4021 acagataaat ggctgacgac ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt
     4081 gctgctcaag cattatcccg gtagggaatt ctgcttctct ggcaattaat gcctgtggcc
     4141 aggtatcatg aaccacacct ttcgcgagca tctcattacc gatatgggtt aaaacttcgc
     4201 tacgatcgac aaaagaaatt ccgctacgaa caaacaggtt agtcatatac cgtccttatt
     4261 ccgcacagcc gtagcgatag gcgcgcaaaa catcataaat tttatccata atcaactgat
     4321 gcggtattgc tgataattcc ccggactgaa tgcgttcaaa ttgtttagga agatactgac
     4381 taatcatgcc cagagggatt tccatgcctt gcagattcac catcatcgtt tcgacgctat
     4441 ttttaatccg gctatgcggc cagtaataac gaatacgatc cgacaggctg taacgaatat
     4501 ccagtaatga atcgttaaaa cccgtacgat aatatttttt ccagtattgc ggttcgtcaa
     4561 acatcacttc ttcaattacc gccaggcaac cgctgcgatt ttctggggcg ataagttcct
     4621 gctcaatttg cgccagcgca aatatcgcct cacgtaaagc aaaggttaat gccggaccga
     4681 ctttcaaaat tgcaaaatga tcgcgaacta attcccgata ggccgtctga gtctggtaat
     4741 cggtggaatg cgcttcataa accattttgg ttttttctat ccattgcgcc agcgcctgag
     4801 cttcttgcgc ctgataatga ataatattgc tatggtcaaa ttccacaccc ggctgcacca
     4861 cgatggcaat cacgcgtgtt aacgcctctg ccagaccgcg ggcaataaac gctttttgat
     4921 gcgtgcgtaa agtattggcg gcatcttcaa cctgggtgat atgtactgac tgaatggcgc
     4981 tggcctcacc gcccggaacc ggaacttcgg tgccaatgac atagctcagt tgctcacgct
     5041 ggcaatctgt cgccacactt tccgcagcaa agcaaagcac agcagctcgt tccgcaaccg
     5101 tttctggggc taacggtata ggatcccccg cgcaagacat tgacgcatca agatgaattt
     5161 tactgaagcc ggcacgaaca tatgccttta ccagttcgac ggatttttcc atcgccgcat
     5221 ccacattttc ttgctgccag cagtttggtc ccagatggtc gccaccgaga ataatacgct
     5281 cacgagcaaa tccgacttta tcggcaatcg caaaaacaaa ttcgcgaaag tctgccggtg
     5341 tcattccggt ataaccgcca aattgattga cctggtttga cgttgcttca atcagcactt
     5401 tacgcgtgct gttgcgatca aatgccagcg ccgcttcgat aaccaacgga tgtgcagaac
     5461 agactgaaca tatgccaata tgttcaccag ctttatgccg ggcaattaac gttttcatgt
     5521 tttttccttg ttaaatggca ggtgcgttat gccctgccct cgcagccaca atcggcaatc
     5581 actttgctca ccacatcgcg cattgcggat ttagccgact gcaaataatc ccggggatcg
     5641 gtcgcttcgg ggtgctctgt caggtaattt tttaacgcct gcgagaaggc attttttagc
     5701 tctgttgcaa cgttgatttt gcatatcccc agtttgatgg tttgctgaat atccttagtt
     5761 gataatcctg acgcgccatg cagcaccagc ggtaagttca cccactggcg aatgttctcc
     5821 agtcgagaaa aatcaagcgc cggtgcgctg gcatacatac cgtgagccgt gccgatggcg
     5881 accgccaggg aatcaattcc ggttgcctcg gcaaattcac gcgcctgaac agggttggtg
     5941 tacaacgcat cggcttcatt gacttgcaca tcatcttcct ggccgccaag ttgccccagc
     6001 tccgcttcga cgctgacatc aaagcgatgg caaaaatcca ccacctcttt gacccgtgaa
     6061 atattttgcg caaaaggcaa atgcgaggcg tcaatcatga ctgagcgcac gccagaacga
     6121 accttctgag cgatatcgtc aaatttcgtg tgatggtcga gatgaattgc cagtggatgg
     6181 tgatattgct tcgccatcgc gctgaccagc gccaacagat tttctgtacc ggcatgagta
     6241 aatgtgccag gcgttccggc gatgatgacc ggcgcatgca ggttggcagc ggtttctacc
     6301 accacttgca tcgtttcgag attgtgaata ttgaatgccg gaaccgcata accgccgcgc
     6361 tgtgcgttgt tcagcatctg ctttgtcgat accacgtaca tttttatatc ctgtcgtttg
     6421 ttttcgattt caaaatataa tgaaatgatt tgtttttaaa tgtcgcgata acgatcacaa
     6481 aaacgacaat atgaaaatca atccaggaat gaaaggcaaa aaaaacggcc tcccggggga
     6541 agccgtaaca aagtacgcgt ggttttatgc cggatgtggc gttaacgcct tttccggcct
     6601 acggggtagt gctgttcgta tgaagcgcat caggcgatag taattttgct atcgagataa
     6661 acgtcctgca cggcgttaat cagtttcacg ccgtcagcca tcgatttctt gaacgcctta
     6721 cgtccaagaa tcagccccat tccgcctgcg cgtttgttga taaccgcagt acgcactgca
     6781 tcgctgaggt cagtttcacc gcctgcagca ccgccggagt ttatcaaccc ggcccggccc
     6841 atatagcagt tagctaactg ataacgcacc aggtcaatcg ggttttcact ggtcagtttg
     6901 ctgtagactc gatcgtcggt ataaccgtaa ttaatcgctt tatagccgcc gttattttcc
     6961 gccatttttt gtttgacgat atctgcacct atggttgccg ccagatggtt cgcctgacca
     7021 gtcaggtcgg cggaaacatg gtaatcaacg ccatctttct taaaggcgga gttacgcaaa
     7081 taggcccaca gcactgtcac catgcccagc tcgtgcgcac gttcaaaagc cgcagaaatt
     7141 tcttcaatct ggcgacgtga ctcttccgag ccaaaataga tagtcgcacc aaccgccacc
     7201 gcgcccatat tgaacgcctg ctccacgctg gcatacagcg tttgatcgta ggtattcggg
     7261 taacttagcg tctcgttgtg attaagtttg acgaggaatg gaatgcgatg cgcgtaacgc
     7321 cgcgataccg atgccagcac gccgtaagtt gacgccacac agttacagcc cgcttcgatc
     7381 gccagttcaa caatgttttt cgggtcaaag tagagcgggt tagcggcaaa tgaagctccg
     7441 gcagagtgct caacgccctg gtcaaccggc agaatagaaa gatatcctgt gccagccaga
     7501 cgcccggtgt tgtacaacgt ctgcatatta cgtaacaccg ctggcgggcg attattgtca
     7561 atcatcacgc ggtctacgta gtcatgtccg gggagataaa gctggtcaga aggaatagtc
     7621 atacaacggt gctgtaaaag gttgtcggcg tctttgccaa gcaactgcgc aatatctgtc
     7681 attactatgc tcccgtaaat tccgattgga tatcggctat ggattttcct ggcccgcctt
     7741 ttgcgggcaa tcataatcct ggtcgttaca ggcgagtttt tccatctttt ggatgttttt
     7801 tcagcgtttt ctgttggcac gattcatcag aaaaaatgtt atcgcggtca agttttcacc
     7861 gcaaaggtat ttaaaaggta ttattaaatg gtattgtcat cgcgtacctt acattacctg
     7921 tcatgaagga attaaaagat gaaaacaaca gcaaagctgt cgttcatgat gtttgttgaa
     7981 tggtttatct ggggcgcgtg gtttgtgcca ttgtggttgt ggttaagtaa aagcggtttt
     8041 agtgccggag aaattggctg gtcgtatgcc tgtaccgcca ttgcggcgat cctgtcgcca
     8101 attctggttg gctccatcac tgatcgcttt ttctcggcgc agaaagtgct ggcggtattg
     8161 atgttcgctg gtgcggcgct gatgtatttc gctgcgcaac agaccacttt tgccgggttc
     8221 ttcccgttac tgctggccta ctcgctaacc tatatgccga ccattgcgct gactaacagt
     8281 atcgcttttg ccaacgtgcc ggatgtggag cgtgatttcc cacgcattcg tgtgatgggc
     8341 actatcggct ggattgcctc cggtctggca tgtggatttt tgccgcaaat gctgggttat
     8401 gccgatatct ctccgactaa catcccgctg ctgattaccg ccggaagttc tgctctgctc
     8461 ggtgtgtttg cgtttttcct gcccgacacg ccgccaaaaa gcaccggcaa aatggacatt
     8521 aaagtcatgc tcggcctgga tgcgctgatc ctgctgcgcg ataaaaactt cctcgtcttt
     8581 ttcttctgtt cattcctgtt tgcgatgcca ctggcgttct attacatctt tgccaacggt
     8641 tatctgacag aagtcggcat gaaaaacgcc accggctgga tgacgctcgg ccagttctct
     8701 gaaatcttct ttatgctggc attgccgttt ttcactaaac gctttggtat caaaaaggta
     8761 ttgttgcttg gtctggtcac cgctgcgatc cgctatggct tctttattta cggtagtgcg
     8821 gatgaatatt tcacctacgc gttactgttc ctcggcattt tgctgcacgg cgtcagttac
     8881 gatttttact acgttaccgc ttacatctat gtcgataaaa aagcccccgt gcatatgcgt
     8941 accgccgcgc aggggctgat cacgctctgc tgccagggct tcggcagttt gctcggctat
     9001 cgtcttggcg gtgtgatgat ggaaaagatg ttcgcttatc aggaaccggt aaacggactg
     9061 actttcaact ggtccgggat gtggactttc ggcgcggtga tgattgccat tatcgccgtg
     9121 ctgttcatga tttttttccg cgaatccgac aacgaaatta cggctatcaa ggtcgatgat
     9181 cgcgatattg cgttgacaca aggggaagtt aaatgaaaac agaacgtatt ctcggtgctc
     9241 tttatgggca ggcgttaggg gatgcgatgg ggatgccctc cgagctttgg ccgcgcagtc
     9301 gcgtcaaagc gtactttggc tggattgacc gttttctgcc tgggccaaag gagaataacg
     9361 cggcctgtta ttttaaccgc gccgaattca ccgacgatac ctcgatggcg ctgtgtctgg
     9421 cggatgcgtt actggaacgt gaaggcaaga tcgatccgga tctgattggg cgtaatattc
     9481 tcgactgggc gctgcgtttc gacgccttta acaaaaacgt actaggtccg acctcgaaga
     9541 ttgctctcaa cgccattcgc gacggtaaac ccgttgctga actggaaaac aacggcgtga
     9601 ccaacggcgc agcgatgcgc gtctcgccat taggttgttt gcttccggcg cgtgatgttg
     9661 attcctttat tgatgatgtg gcgctggcct ccagcccgac acataaatcc gatctggcgg
     9721 ttgcgggggc ggtagtcatc gcatgggcga tttctcgtgc cattgacgga gaaagctggt
     9781 cagcgattgt tgattcactg ccttcaattg cgcgacatgc tcaacaaaaa cgcatcacta
     9841 ccttcagcgc ctcactggca gcacgtctgg agattgcgct gaaaattgtg cgcaatgccg
     9901 acggcaccga atccgccagc gaacagcttt accaggtcgt tggcgcaggt accagcacta
     9961 ttgagtccgt tccgtgcgcc attgcgctgg ttgaactggc acaaaccgac ccgaaccgtt
    10021 gcgccgtcct gtgcgctaac cttggcggcg acacagacac catcggtgct atggcgacgg
    10081 caatttgtgg cgcgttgcat ggcgttaacg ctatcgatcc tgcgttaaag gcggaactgg
    10141 atgcagtaaa tcagcttgat ttcaaccgct atgccacggc gctggcgaag tatcgccaac
    10201 aacgggaggc gatatgagcg gcgctcgatt acacacgctg ctgcctgaat tagccacgcg
    10261 tcagccagtg atggtcgtcg gcgcggcggt cattgatgtg atcgccgacg cttatgccct
    10321 cccctggcgt gggtgcgata tcgaactgaa acagcagagc gttaacgttg gcggctgcgc
    10381 actgaatatt gccgtggcgt taaagcgcct cggcatcgaa gcgggtaatg ccttgccgct
    10441 cggtcagggc gtgtgggcgg agattattcg caaccggatg gcaaaagagg gcttaatcag
    10501 cctgatcgat aacgccgaag gtgataacgg ctggtgtctg gcgctggttg agccggatgg
    10561 cgaacgcact tttatgtcat tcagcggtgt ggaaaatcag tggaatcgcc agtggctggc
    10621 gcgattaacc gttgcgcctg gcagcctgct ctatttttcc ggttatcaac tggcctcgcc
    10681 ctgcggcgaa ttgttagtgg aatggctgga agagctgcaa gacgtgacgc catttatcga
    10741 ttttggccca cgtattggcg atatcccgga tgcattactg gcgcggatca tggcctgtcg
    10801 acctttagtg tcactcaatc gtcaggaggc tgagattgcc gccgaacgtt ttgctttatc
    10861 cgcagagata acaacacttg gcaagcaatg gcaggagaaa tttgctgcga cgttgatcgt
    10921 tcgtctcgat aaagagggcg catggtattt cagcaacgac gcttctggct gcattccggc
    10981 atttccaacg caagttgtag acaccattgg ggcgggcgac agtcatgccg gcggcgtact
    11041 tgccgggctg gcctctggtc tgccactggc ggatgccgta ttactgggca atgcagtggc
    11101 gtcgtgggtt gtcgggcatc ggggcggtga ttgtgcgcca acgcgcgagg aactactcct
    11161 cgcacacaaa aacgtataga tcgctgcgac agtggctgat gctgtactca ataggccgtt
    11221 gctgttggtc aagcgccact tgcttgatca ccagcaccgg tattttgcta tctaactgaa
    11281 tatgtgactg aaactcggca tccggcatcc gggcgctaac gcgggaacgc gtacgttgtg
    11341 ggtaaatgtg ctggctgcgg aagtagtcat aaagagaaat cccgatggca tcaacatcat
    11401 gaattaaatg tgccggaacc cacgattcct caatcgatac cgcctcttca tcgacatagc
    11461 gaatacgctt aagcaaaaag acatcacttc ctgcttcgac cgctagctgc tgcgcgactt
    11521 cttcgggaca tttcacgaca cgcttgttaa cccatagcgt atcgggcttt ttcccgcgca
    11581 ataccacctg ctgagaaaaa cctcgcgctt ctttcaacga gtattcgaag atgttgttga
    11641 tctgcgtgcc gtaaccacgc gaacgcgtca ccacgccctc ttcttccagc gcctgcatcg
    11701 ccttgcgcac cgtaatgcgc gaaacgccgg ttaactgact taaatcacgc tcaccgggca
    11761 aaatattgcc atgctccagc accccgctgc gcacggcatt ttttaccgtt tcggcaaact
    11821 taatataaag cggcgtgtta tccgccgcta aaatacgttc gttcagttgg gcaatcagct
    11881 gggtatgcgc ttgttccatt tgtctttttc ctgccaggga gtctggtggg agtatatcac
    11941 tgcttcttat ttaaggcgtt tctttaatcc catcaacgaa tccctgcaac tcctccaccg
    12001 tgccattaaa cacattaaaa tccaccggac cattaatgcc atctacctgt ccacggtcgg
    12061 aatgctgcca gaagcgccag gccattccgt cattgtccgg acgacgttga tagtagtgag
    12121 ccacccacca cggatattca ttaaaatagc ccgccagatt ggtgtgataa aaaacggctc
    12181 ctgagtaaat aattggcttt tttcccgtac gtttttcgac catttttagc cactgactta
    12241 cccgcttgcg taattctttt gccgataatt ttcctcgctc ttctacgtcc agtaccgcag
    12301 ggaaatcgcc ttgtgagaaa tccaccgttt gcagaaataa tcgcgcctga actgaagcgg
    12361 atactgacgg ggagaaataa tgatacgccc cgcgcagcag gccattttcg cggcttagtt
    12421 gccagttacg cgaaaaatag gggtccacca gcttttcgcc ttccgtcgcc ttaataaaag
    12481 caaactgtaa gcggataccg ttgtcgcgca tttttgccac ccgctgccag tcgatccgct
    12541 cctgccagcg cgaaacatct attccgtgaa tggtgtaact ggcaggaatg cgaatagcga
    12601 aagatttaac cgggcgataa ccgtagaagt ggatgtagtc tttaatctga attgctgtcg
    12661 aatagaaaaa attcaccgtc tgacgcggat aaattgcaac gatggaaatt agcccaagag
    12721 cgcataataa agcggtgaat ttttttcgac tggtgattct tagctgcatt ggtttcatcc
    12781 ctgaatgtca gcgccaaagg ctgacaataa ccaaagcagc tatagtacgg tgctcaccac
    12841 caggcgtgga agtggtgaac cggaccaata ccgtgaccaa cttccagcgt gtcggcctgg
    12901 gctaatgccg atgaaagcca gctttttgcc tcctgtacgg tgtcagtcca gtttgtatgg
    12961 cgcgggcgca gtgcagccaa cgccgcagag agcgtacaac cagtgccgtg agtgtttttg
    13021 gtcataatgc gctgtgcggt aaaccgttgt tcaccctcgc gggtaaacag ccagtccggg
    13081 ctttgctcat catccagatg accacctttc attagcactg cgccacagcc catcgccaac
    13141 agcgatcgcc cttgttccag catttcctgt tcggtgcgcg cgtgtggcgc gtcgagcaag
    13201 gcggctgctt cgggcaagtt tggcgttatt aatgaaacct gtggcaataa tcgactgcgc
    13261 agcgtagcaa ccgccgaagg tgaaagcagc gggtcgccgc tttttgccag cataacggtg
    13321 tcgagtacca cgttttggat ctgataacgt tgcaaccgtt ctgccaccgc ttcaacaata
    13381 tcggtttccg ccaacatacc gattttagtg gtgtcgattc gcacatcgct gaacaccgaa
    13441 tcgagctggg cggcgacaaa atcaggctca atgcgataca ccgactgtac accacgggta
    13501 ttttgcgcca ccagtgcagt aataactgag caaccataag cgccaagtgc cgagaaggtt
    13561 ttaagatcgg cctgaatccc cgcaccacca ctcggatcag taccggcaat cgtcagagcg
    13621 ttaattcgtt tcacgcttgc acctcctgcg tcaattgcca gagcgcatca aggaaatgtg
    13681 gaacaaaact gcctggcccc tcgctactgg cgactgcgcg ttctccggct tgtttcatcc
    13741 agtgacaggc agatgcgata ttttccagcg tatcgcctgg tagcgcacaa caggcagcga
    13801 caaccgccga taatgcacag ccagttccta ccactttggt cattaacgga tcgccaccgt
    13861 gaatgccaat gatacgatgt ccatcggtaa cataatccac ctcgccagtg accacgacga
    13921 ttgcgccagt ttcccgtgcc agtgtttgtg cagcgggtat cgcgttagct gcggcatcag
    13981 tggtatccac tccccgtcca ccattagcaa cgccagctaa tgccatgatt tccgaagcat
    14041 taccacgtat ggcggttggc ttatgggaca gaagttccac acaaaaacgg cggcgataat
    14101 cgagcgcacc aaccgcaacc ggatcaagcg tccagggcgt ttgtgatcgt gttgcttgct
    14161 caacggcagc actcatcgct tgcgctcgtg gctgcgtcag cgtgccaacg ttaatcaaca
    14221 aggcactggc gatagccgca aactgactgg cctcttcggt ttcgataacc atcgctggcg
    14281 atgcaccgat cgccagcaag gtattggcag taaaagtttg caccacatcg ttggtcatgc
    14341 agtgcacaag aggggaatgt tggtgaaaaa ggtgtaacgt gtgcgcagat tgcgcggaac
    14401 tcagcaggtc gacttgcata gtttgctcct gccataacgt gaagaagcaa tgacctggtg
    14461 gtccgtgact tccctacgct ggcattatcc agatcaggtg atacgggtat ttctcagcct
    14521 tcacaaagaa gggcaccccg agtcgtttgg ttgcgataat aaatcgcaga ggaggatggt
    14581 aatgtccagc gcacgcgttg taaacgggga aaaacggcgg tattacaatc gcgttaataa
    14641 attatttgat ataagaatcc agcaccttca gaacgacatc cagatcttct tcacgtttta
    14701 gctcatcccc ctggtgaacg atgtgttccg tcagatggcc tttaatcact tcccgcatca
    14761 gaccgtttac cgcgccacgg atagcagcaa tctgttgtaa aactgcagcg cattcatgcg
    14821 gttcgtcgag cattttcttg agcgccacga cctggccctg aatcttactg gcacgcgctt
    14881 tcagtttctg tttatcacgg attgtatgag acatggcaac acctggttaa caagaatatg
    14941 aaaaatcata gcactattaa tctactgggg ggtagtataa ggtactgggg ggggagtaga
    15001 atcagattgc cgaattaata ctaagaatta ttatcatgac cgaatttaca acacttcttc
    15061 agcaaggaaa cgcctggttc ttcatcccca gcgccatctt acttggtgcg cttcatggcc
    15121 tggaaccggg gcactcaaaa acgatgatgg cggcgtttat catcgccatc aaaggcacca
    15181 ttaaacaggc ggtgatgctc ggactggcag caactatttc gcataccgca gtagtctggt
    15241 taattgcctt tggcgggatg gtgatcagca aacgctttac tgctcaatca gcagaaccgt
    15301 ggctccagtt gatttccgca gtgatcatta ttagcaccgc gttctggatg ttctggcgta
    15361 cctggcgcgg caaacgcaac tggctggaga atatgcacga gcatgatcat gagcatcatc
    15421 accacgatca cgaagatcac cacgaccatg gacatcatca ccatcacgaa catggcgagt
    15481 atcaggatgc ccatgcacga gcccatgcca atgacattaa acgacgcttt gatggtagag
    15541 aggtcaccaa ctggcaaatt ttgttatttg gcttaaccgg tggccttatc ccctgcccgg
    15601 cagcaattac cgtgctgttg atttgcattc agttgaaagc cctgacactg ggcgcaacac
    15661 tggtcgtcag tttcagcatc ggcctggcgt taacgcttgt caccgtaggc gttggcgcag
    15721 caatcagcgt tcagcaggtc gcaaaacgct ggagcggatt taacactctc gctaaacgcg
    15781 ctccctattt ttccagtctg ttgattggct tagttggtgt gtatatgggc gtacatggct
    15841 tcatgggcat aatgcgataa ctcaatcctt taggcttgca tgttatcttc agcccaggat
    15901 gattcttaaa tcagctattt ccgctgactc tctgctcgct aaggatgctt ttagggcatc
    15961 ctttcattta cactttttac gaaatcatgg gatcactaac aaaatatcgc ttgtcagtta
    16021 tattgtatgg caggaaagat atgcgactga tattacagat ccccaaagtg gagagtttat
    16081 gaccattaaa aataagatgt tgctgggtgc gcttttgctg gttaccagtg ccgcctgggc
    16141 cgcaccagcc accgcgggtt cgaccaatac ctcgggaatt tctaagtatg agttaagcag
    16201 tttcattgct gactttaagc atttcaaacc aggcgatacc gtcccagaca tgtaccgcac
    16261 cgatgagtac aacattaagc agtggcagtt acgtaacctg cccgcgcctg atgccgggac
    16321 gcactggacc tatatgggtg gcgcgtacgt gttgatcagc gacaccgacg gtaaaatcat
    16381 taaagcctac gacggtgaga ttttttatca tcgctaaaaa aagccccctc atcatgaggg
    16441 ggaaatgcag acaccttgtt attttttatt attcgccact tgctcgtctt gcttattatt
    16501 agtcgtattt cacattgatt aatgcggttg cctgtagtgc accagatttt actttatttt
    16561 tatcataaac atagtaactg gcagttactg gaattgagac cgttgtatct gtaatttttg
    16621 acttcgccgc aatttctttt aaatttgtgt accctttgct gttatcagtg atatttaaaa
    16681 atgtgactgg cgaactattg ctttcaagca taaatccgac tccattatct tctgcacctt
    16741 tcaggatcgt gttactgccg gctaacaaat cagaacttga taaatacacc tgcatttttc
    16801 gtgatgtttg attttgttga cctaataaat ttttacaacg cagaataata tccccttttt
    16861 tactatttat tgttgctttg ttacctgtgg ctggtaattg tgaaacggga atactctcta
    16921 aattaattaa taaatcgtcc ggtttgcagg ttgtggttat cgggttgtat gacaatgcca
    16981 aattaaagga atagacacct gcattttgtg ccaatgcatt tctgtagcta tttacacatg
    17041 catcccatga cttgccacct aataaacaca gtagaaattg tactgcggca ctcccgcgtg
    17101 tcgatgatga agcactggat aatgttgcaa caccattaat catcactgaa ccgccgcccc
    17161 ccgcagaaac attcactttt gcattactgg ccgcaggtgt caccgttacc gaataaccac
    17221 tggtgaaact ggtctgatta tttgacaacg taatgttgtt tttattggtc cattcaaaat
    17281 gtaaatttaa tttaatggta tcgttataaa gtccaacaat aatatccgac gggctcatag
    17341 gtgtgatagt atttgtactc acgcatgacg aatcattaaa attatgctca tatgtcgcca
    17401 tttgtgtttg agtaagtgca aaatccccct gaacaaccaa attgtgctga acagataact
    17461 cactataaca tgtcgcatag ctcacaggca taaccatcat cattactata aatagcatta
    17521 tgcgaatttc catagaacca cctgactatt actggcaaat ataatttcta ctttcatcaa
    17581 tctctttacc gaaggtgatt gcgcatgaaa gcccttgttg cttatcaatt gccacattaa
    17641 ccgatggcgg tacttcattg gtgcgaataa ataactggct tccctggccg acaacaccaa
    17701 tattatgacc atggatatca ttgacttcat aaccaaacgt taatggttgc ccatccgttc
    17761 ttaacgcttt tataaaccag ggcttgcgct gatcggtatc aaaattaacc agtacaaccg
    17821 cgccgcgaaa aggggcggca attttccggt tgccacgtaa ttctgcttcg ctatcgcttt
    17881 gcgacacatc cagcatcagg tgattttccc gataaggtgt cattccgtcg tataccacca
    17941 ctccattacg gtttgttgtg cgatattttt gcccattgac ataagcatct ttaattcctg
    18001 gcgcattcat tacagcaaac gtttcggaaa gacggttcgc cagattaacg ccacccgacc
    18061 aggcgacaat gccccctgaa acactggctc cagcctgtcg ataagtactc gactgactat
    18121 aactgccatt caccgttgcc accggcgcgt tccaggttaa attcgctcca gctgtcgttt
    18181 catttccctg atgctgataa ctcaggttga ccccatagtt aaactgatcc cggcttccaa
    18241 cggttcctga taatcccgta ttatttgagg caaccccctg atcatcaaac gtcgttgagt
    18301 tagacatata tatttgccga cgaggcgtcg taacgtcatc accccaatca aagggaatcg
    18361 atataaaaat attaaaacgt ttctcttcat gatgattctc gtcataagca tggcttgccg
    18421 cgagggtata gcttatcctt cgcaggttgt tggaataact caactgataa tccttgctgc
    18481 tgccgctacg cccccagtaa tctcgccata acgtacttaa ggacacagaa ccccagcctt
    18541 ctggcaatga ctggctcata ttggcagaaa agctattttt acggccaaaa tcgttctggt
    18601 aataatcggc aatgtcatag atatcgtttt catcacggcg ataattatct ttattgtttg
    18661 cccatacgtg atcattaaat gtccgataat cacgcgacga ataacgccag gccgccagac
    18721 caaaacgtgt cgacgtttgg ctcacaaatt tgttgtaggc aatttgataa ctttgcccgt
    18781 caaacacatc gccgttgtct tgtatactat gcgacttcgt ggcatcgacg gaaatggcac
    18841 caatgcgtgt attccagcca gtccccaaag tgaacgcgta ataattattc gcgaccatcg
    18901 atccgccata cagcgtcaat aagttattaa aaccatactg ataacccgcc tgaacaaaat
    18961 cactttgttt gctcgcccct tcaatatggc tacgacctgc cgcaaaatca tatttcgaca
    19021 cgccgggttg cagcatattc ggcactgctg cataaggcac cagataggtg gttaccgaac
    19081 cgtccgcctc tttcacgctg acatcaagat ctgctccacc accggccaac tgcaaatctg
    19141 taatcgcaaa cgggccagga ggaacctctt tctgataaac cacaaaacca ttctgttcaa
    19201 tagttaccag cgcgttactc tgagcaatcc cctgcacccg tggagtaaaa ttttgtttcg
    19261 agttaggcaa catctgcata tcacgaaaca accgcacacc gctgaagcga acagaatcaa
    19321 aaatatcgct tgatgtgtac atatcaccca cgcgaagcgt gccgagaagt tgggcaaatc
    19381 cacgttccag atacagggta ttgcttttcc acacccctgg attgctattt gttttactga
    19441 aactggcatc agaatgcagt tgccacccca gtaagtttaa cccgctgtta aaacgtacat
    19501 atgtactctt gctattaccc gacgctttat agtcgctgta atactgactc acataataag
    19561 aggtataaaa cgcattaata ccccgctccc agttttccgg tggaacatag ccactttcca
    19621 gttcttccac ccacgcctgc gggacactga aatcgagacg aaaaactccg atatcccagg
    19681 tatagctccc accctgaaca agttgctcaa atgttaaaca ttgcttaccg ctggcaaagt
    19741 tatcggtatt aatgcctaac cgcttgataa tttcccttga taaacatgtt tcttgcgggt
    19801 tgtctttaac aataatctca tatttcccgc gccattgctt attgacataa atatcgatgt
    19861 catactgccc cggcaagggt tgattatcat caagacgaat atttgcaacc tgctggtctt
    19921 tcattccacc tatcatgaaa tgggtatcaa aggtttcttc agctgcataa gcttcaatgc
    19981 cgagcaataa cgctacgatt gctgatgcaa gtggggtcat tctcaacata ggcagctcct
    20041 gcaattaaat tttgtcacta atatagttgc catggtcatc gataatggtc agatgccagt
    20101 tattcgcatt attacttttg acattaatac tctgactttc taagggggca atcataatag
    20161 tttcataatt gactttgaca ttattagctt tgacatccga aatcgttacc caattttctg
    20221 agtcattttt tatcaccaat ccattgccac tgcgatttac cagtaacttt ttaaatgtcg
    20281 ctttatttac cggagcaata cccgctggcc ggtaaaacaa cttaattctg ttttgcatcg
    20341 caaacttcag tgcattctta ccttcttgtt ctggact
//
LOCUS       DAZGAP010000094        20268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGAP010000094 DAZGAP010000000
VERSION     DAZGAP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.094"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            192..1667
                     /gene="zwf"
                     /locus_tag="ACHLAM_002917"
     CDS             192..1667
                     /gene="zwf"
                     /locus_tag="ACHLAM_002917"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO7854416.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            1902..3713
                     /gene="edd"
                     /locus_tag="ACHLAM_002918"
     CDS             1902..3713
                     /gene="edd"
                     /locus_tag="ACHLAM_002918"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO7854417.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            3750..4391
                     /gene="kdgA"
                     /locus_tag="ACHLAM_002919"
                     /gene_synonym="eda"
     CDS             3750..4391
                     /gene="kdgA"
                     /locus_tag="ACHLAM_002919"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO7854418.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4447..5625)
                     /gene="purT"
                     /locus_tag="ACHLAM_002920"
     CDS             complement(4447..5625)
                     /gene="purT"
                     /locus_tag="ACHLAM_002920"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO7854419.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            5759..6049
                     /gene="yebG"
                     /locus_tag="ACHLAM_002921"
     CDS             5759..6049
                     /gene="yebG"
                     /locus_tag="ACHLAM_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO7854420.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            6116..6472
                     /gene="yebF"
                     /locus_tag="ACHLAM_002922"
     CDS             6116..6472
                     /gene="yebF"
                     /locus_tag="ACHLAM_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO7854421.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQPDPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            6799..7458
                     /gene="yebE"
                     /locus_tag="ACHLAM_002923"
     CDS             6799..7458
                     /gene="yebE"
                     /locus_tag="ACHLAM_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7854422.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            7667..9727
                     /gene="ptrB"
                     /locus_tag="ACHLAM_002924"
     CDS             7667..9727
                     /gene="ptrB"
                     /locus_tag="ACHLAM_002924"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO7854423.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(9724..10386)
                     /gene="exoX"
                     /locus_tag="ACHLAM_002925"
     CDS             complement(9724..10386)
                     /gene="exoX"
                     /locus_tag="ACHLAM_002925"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO7854424.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(10410..11066)
                     /gene="yobB"
                     /locus_tag="ACHLAM_002926"
     CDS             complement(10410..11066)
                     /gene="yobB"
                     /locus_tag="ACHLAM_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO7854425.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(11168..11398)
                     /gene="holE"
                     /locus_tag="ACHLAM_002927"
     CDS             complement(11168..11398)
                     /gene="holE"
                     /locus_tag="ACHLAM_002927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO7854426.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            11537..11911
                     /gene="yobA"
                     /locus_tag="ACHLAM_002928"
     CDS             11537..11911
                     /gene="yobA"
                     /locus_tag="ACHLAM_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO7854427.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            11915..12787
                     /gene="copD"
                     /locus_tag="ACHLAM_002929"
                     /pseudo
     CDS             11915..12787
                     /gene="copD"
                     /locus_tag="ACHLAM_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            12800..13141
                     /gene="yebY"
                     /locus_tag="ACHLAM_002930"
     CDS             12800..13141
                     /gene="yebY"
                     /locus_tag="ACHLAM_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO7854428.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            13536..14192
                     /gene="pphA"
                     /locus_tag="ACHLAM_002931"
     CDS             13536..14192
                     /gene="pphA"
                     /locus_tag="ACHLAM_002931"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7854429.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWFMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(14193..14384)
                     /gene="yebW"
                     /locus_tag="ACHLAM_002932"
     CDS             complement(14193..14384)
                     /gene="yebW"
                     /locus_tag="ACHLAM_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO7854430.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14489..14725)
                     /gene="yebV"
                     /locus_tag="ACHLAM_002933"
     CDS             complement(14489..14725)
                     /gene="yebV"
                     /locus_tag="ACHLAM_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO7854431.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(14843..16282)
                     /gene="rsmF"
                     /locus_tag="ACHLAM_002934"
     CDS             complement(14843..16282)
                     /gene="rsmF"
                     /locus_tag="ACHLAM_002934"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO7854432.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(16362..18995)
                     /gene="yebT"
                     /locus_tag="ACHLAM_002935"
     CDS             complement(16362..18995)
                     /gene="yebT"
                     /locus_tag="ACHLAM_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO7854433.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(18964..20247)
                     /gene="yebS"
                     /locus_tag="ACHLAM_002936"
     CDS             complement(18964..20247)
                     /gene="yebS"
                     /locus_tag="ACHLAM_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO7854434.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
BASE COUNT         5069 a         5105 c         5202 g         4892 t
ORIGIN      
        1 gatttttacc caatggaaaa cgatgatttt tttatcagtt ttgccgcact ttgcgcgctt
       61 ttaccgtaat cgcacgggtg gataagcgtt tacagttttc gcaagctcgt aaaagcagta
      121 cagtgcaccg taagaaaatt acaagtatac cctggcttaa gtaccgggat agttaactta
      181 aggagaatga catggcggta acgcaaacag cccaggcctg tgacctggtc attttcggcg
      241 cgaaaggcga ccttgcgcgt cgtaaattgc tgccttccct gtatcaactg gaaaaagccg
      301 gtcagctcaa cccggacacc cggattatcg gcgtagggcg tgctgactgg gataaagcgg
      361 cttataccaa agttgtccgc gaggcgctcg aaactttcat gaaagaaacc attgatgaag
      421 gtttatggga caccctgagt gcacgtctgg atttttgtaa tctcgatgtc aatgacactg
      481 ctgcattcag ccgtctcggc gcgatgctgg atcaaaaaaa tcgtatcacc attaactact
      541 ttgccatgcc gcccagcact tttggcgcaa tttgcaaagg gcttggtgag gcaaaactga
      601 atgctaaacc ggcacgcgta gtcatggaga aaccgctggg gacgtcgctg gcgacctcgc
      661 aggaaatcaa cgatcaggtt ggcgaatact tcgaggagtg ccaggtttac cgtatcgacc
      721 actatcttgg taaagaaacg gtgctgaacc tgttggcgct gcgttttgct aactccctgt
      781 ttgtgaataa ctgggacaat cgcaccattg atcatgttga gattaccgtg gcagaagaag
      841 tggggatcga agggcgctgg ggctattttg ataaagccgg tcagatgcgc gacatgatcc
      901 agaaccacct gctgcaaatt ctttgcatga ttgcgatgtc tccgccgtct gacctgagtg
      961 cagacagcat ccgcgatgaa aaagtgaaag tactgaagtc tctgcgccgc attgaccgct
     1021 ccaacgtgcg cgaaaaaacc gtacgcgggc aatatactgc gggcttcgcg cagggcaaaa
     1081 aagtgccggg atatctggaa gaagagggcg cgaacaagag cagcaataca gaaaccttcg
     1141 tggcgatccg cgtcgacatt gataactggc gctgggccgg tgtgccattc tacctgcgta
     1201 ctggtaaacg tctgccgacc aaatgttctg aagtcgtggt ctatttcaaa acacctgaac
     1261 tgaatctgtt taaagagtcg tggcaggatc tgccgcagaa taaactgact atccgtctgc
     1321 aacctgatga aggcgtggat atccaggtac taaataaagt tcctggcctt gaccataaac
     1381 ataacctgca aatcaccaag ctggatctga gctattcaga aacctttaat caaacgcatc
     1441 tggcagatgc ctatgaacgt ctgctgctgg aaaccatgcg tggtattcag gcactgttcg
     1501 tacgtcgcga tgaagtggaa gaagcctgga aatgggtaga ctccattact gaggcgtggg
     1561 cgatggacaa tgatgcgccg aaaccgtatc aggccggaac ctggggaccc gttgcctcag
     1621 tggcgatgat tacccgtgat ggtcgttcct ggaatgagtt tgagtaatat ctgcgctcat
     1681 cctttatggt tattttaccg gtaacatgat cttgcacaga ttgtagaaca atttttacac
     1741 tttcaggcct cgtgcggatt cacccacgag gcttttttta ttacactgac tgaaacgttt
     1801 ttgccctatg agctccggtt acaggcgttt cagtcatgaa tcctctgaat gaaacgtgtt
     1861 gtgaatcatc ctgctctgac aactcaattt caggagcctt tatgaatcca caattgttac
     1921 gcgtaacaaa tcgaatcatt gaacgttcgc gcgagactcg ctctgcttac ctcgcccgga
     1981 tagaacaagc gaaaacttcg accgttcatc gttcgcagtt ggcatgcggt aacctggcac
     2041 acggtttcgc tgcctgccag ccagaagaca aagcctcttt gaaaagcatg ttgcgtaaca
     2101 atatcgccat catcacctcc tataacgaca tgctctccgc gcaccagcct tatgaacact
     2161 atccagaaat cattcgtaaa gctctgcatg aagcaaatgc ggttggtcag gttgcaggcg
     2221 gtgttccggc gatgtgtgat ggtgtcaccc aggggcagga tggaatggaa ttgtcgctgt
     2281 taagccgcga agtgattgcg atgtctgcgg cggtggggct gtcccataac atgtttgatg
     2341 gtgctctgtt cctcggtgtg tgcgacaaga ttgtcccggg tctgacgatg gcagccctgt
     2401 cgtttgggca tttgcctgcg gtgtttgtgc cgtctggacc gatggcaagc ggtttgccaa
     2461 ataaagaaaa agtgcgtatt cgccagcttt atgccgaagg taaagtggac cgcatggcac
     2521 tactggagtc agaagccgcg tcttatcatg cgccgggaac atgtactttc tacggtactg
     2581 ccaacaccaa ccagatggtg gtggagttta tggggatgca gttgccaggc tcttcttttg
     2641 ttcatccgga ttctccgctg cgcgatgctt tgaccgctgc agctgcgcgc caggttacgc
     2701 gcatgaccgg taatggtaat gaatggatgc cgatcggtaa gatgatcgat gagaaagtgg
     2761 tggtgaacgg tatcgttgca ctgctggcga ccggtggttc cactaaccac accatgcacc
     2821 tggtggcgat ggcgcgcgca gccggtattc agattaactg ggatgacttc tctgaccttt
     2881 ctgatgttgt accgctgatg gcacgtctct acccgaacgg tccggccgat attaaccact
     2941 tccaggcggc aggtggcgta ccggttctgg tgcgtgaact gctcaaagca ggcctgctgc
     3001 atgaagatgt caatacggtg gcaggctttg gtctgtctcg ttataccctt gaaccatggc
     3061 tgaataatgg tgaactggac tggcgggaag gggcggaaaa atcactcgac agcaatgtga
     3121 tcgcttcctt cgaacaacct ttctctcatc atggtgggac aaaagtgtta agcggtaacc
     3181 tgggccgtgc ggttatgaaa acctctgccg tgccggtaga gaaccaggtg attgaagcgc
     3241 ctgcggttgt ttttgaaagc cagcatgacg ttatgccggc ctttgaagcg ggtttgctgg
     3301 accgcgattg tgtcgttgtt gtccgtcatc aggggccaaa agcgaacgga atgccagaat
     3361 tacataaact catgccgcca cttggtgtat tattggaccg gtgtttcaaa attgcgttag
     3421 ttactgatgg acgactctcc ggcgcttcag gtaaagtgcc gtcagctatc cacgtaacac
     3481 cagaagccta cgatggcggg ctgctggcaa aagtgcgcga cggggacatc attcgtgtga
     3541 atggacagac aggcgaactg acgctgctgg tagacgaagc ggaactggct gctcgcgaac
     3601 cacacattcc tgacctgagc gcgtcacgcg tgggaacagg acgtgaatta ttcagcgcct
     3661 tgcgtgaaaa actgtccggt gccgaacagg gcgcaacctg tatcactttt taagacgaca
     3721 aatttgtaat caggcgagag aaaactctga tgaaaaactg gaaaacaagt gcagaatcaa
     3781 tcctgaccac cggcccggtt gtaccggtta tcgtggtaaa aaaactggaa cacgcggtgc
     3841 cgatggcaaa agcgttggtt gctggtgggg tgcgcgttct ggaagtgact ctgcgtaccg
     3901 agtgcgcagt tgacgctatc cgtgctatcg ccaaagaagt gcctgaagcg attgtgggtg
     3961 ccggtacggt gctgaatcca cagcagctgg cagaagtcac tgaagcgggt gcacagtttg
     4021 caattagccc gggtctgacc gagccgctgc tgaaagctgc taccgaaggg actattcctc
     4081 tgattccggg gatcagcact gtttccgaac tgatgctggg tatggactac ggtttgaaag
     4141 agttcaaatt cttcccggct gaagctaacg gcggtgtgaa agccctgcag gcgatcgcgg
     4201 gtccgttctc ccaggtccgt ttctgcccga caggtggtat ttctccggct aactaccgtg
     4261 actacctggc gctgaaaagc gtgctgtgca tcggtggttc ctggctggtt ccggcagatg
     4321 cgctggaagc gggcgattac gaccgcatta ctaagctggc gcgtgaagct gtagaaggcg
     4381 ctaagctgta aaagtcaaaa tgcccgatcg aggatcgggc atttttgtag cgtttttttg
     4441 ccgggtttaa ccctgtactt ttacctgtcc ggcggcgtgc tttgcgcgct caatcgcttc
     4501 atcaacactc tcagcggtcg ccagcgcaac acccagacga cggctgccat caatttccgg
     4561 cttaccaaat aaacgaatct gtatgcctgc gcctacggca ttctgcacat tatcaaacgt
     4621 gacattctga ctggtcagtt gtggcaggat aacggcagaa gctgcaggac catactgacg
     4681 gatcccgcca accggaagtc cgagaaaggc tcgcacatgc agggcaaact ccgagagatc
     4741 ctgagaaatt aacgtcacca tcccggtatc atgtgggcga ggggagacct cactgaaaat
     4801 cacctcatca ccacagacaa acagctcgac accaaacagc ccataaccgc ccagtgccag
     4861 caccacttta cgggcaatct cttgcgcacg ttccaaagcc agctggctca tttgctgtgg
     4921 ttgccaggat tcacgatagt cgccatcttc ctggcgatga cctaccggtg cacagaaatg
     4981 gacgccatcc accgcgctga cggttagcag ggtaatttcg aagtcaaact taacgacgcc
     5041 ttcaacaatt acgcggcccg ctccggcgcg accgccttgc tgagcgtact cccatgcatg
     5101 agcaagttgc tctgcagaac gaataaacgt ctgccccttg ccggaggaac tcatcaccgg
     5161 tttaacaatg cagggatagc cgatttcagc aaccgcctcg cggaaaaggt tttcgttatc
     5221 ggcaaaacga taagtggaag tgggcagctg cagctcttct gctgccaggc gacggatacc
     5281 ctcgcgattc atcgttaatt tcgttgcgcg agcgcagggg acaacattca gtccttcctc
     5341 ttcaagttgg atcagcatat cggtggcaat agcttcgatc tccggcacga tataatgtgg
     5401 tttttccagt tcaaccacac ggcgtaatgc atcaccatca agcatattaa tgacatggga
     5461 gcgatgcgcg acatgcatgg ctggtgcgtc ggcatagcga tcgacggcaa tcacctctac
     5521 gccgagacgc tgacactcga ttgccacttc tttacccagt tcaccggagc ctaataacat
     5581 cacgcgagtt gctgccggac gcagcgcagt gcctaataac gtcataacga atatcccctg
     5641 atttattccg cgcgcagtat aaacgaaaac gtttgcgcgt gtctttattg ttgatgttga
     5701 tttcccaatc gagaagaaat atactgtata aaatcacagt tattatgaga ggctgcaaat
     5761 ggcggttgaa gttaaatacg tagtcattcg tgagggcgaa gagaaaatgt cgtttaccag
     5821 caaaaaggaa gccgatgctt atgacaaaat gcttgatacg gcggatctgc ttgacacctg
     5881 gctgacaaat tctccagtgc aaatggaaga cgagcaacgt gaagcccttt cgctatggct
     5941 ggcagaacaa aaagatgtgc tgagcaccat tctgaaaacc ggcaaattac cgtccccaca
     6001 ggtcgtcggt gctgaatccg aagaggaaga tgcctcacac gcggcctgaa gcaacttgcg
     6061 ctcctgacaa tttgtaccat gctttctcct gaatcattgt gctatggaga aaaacatgaa
     6121 aaaaagaggg gcgtttttag ggctgttgtt ggtttctgcc tgcgcatcag ttttcgctgc
     6181 caataatgaa accagcaagt cggtcacttt cccaaagtgt gaaggtctgg atgctgccgg
     6241 aattgccgcg agcgtaaaac gtgattacca acaaaatcgc gtggcgcgct gggctgatga
     6301 tcaaaaaatt gtcggtcagc ccgatcccgt ggcatgggtc agtttgcagg acattcaggg
     6361 gaaagatgat aaatggtcag taccgctaac cgtacgtggt aaaagtgccg atattcatta
     6421 ccaggtcagc gtggactgca aagcgggaat ggcggaatat cagcggcgtt aatggcttct
     6481 gataattcga ttgaccgttg gcggatacgg cgcgagtgcc ttgtccgccc tacaacgcaa
     6541 cacaatcgta gtcctgagaa gacgcagcag cgtcgaaccg ggcaatcagc atcaaacagt
     6601 tccactcaaa acgtcaaact actttcacct aaaataccct tatcacctta tatacagtga
     6661 acaccttacc tggcgcgaat ttctttgaat aacggcctac cgccccgctt atttgcgcat
     6721 cttttgtctc aatatacatt ttctgacatc ctcatccgct atgctacagg cactaaaaat
     6781 ggttattgag gagcaaatat ggctaactgg ttaaatcaac tgcaatccct gcttgggcaa
     6841 agccgttctt ctacctcctc gtctgcggat cagggattgg gcaaactttt agtgcctggc
     6901 gcattaggcg gactggctgg gctgctggtc gcaaataaat cagcacgtaa acttcttacg
     6961 aaatatggca caaacgcgtt actggttggc ggcggagcgg tagcgggtac ggtgctgtgg
     7021 aataaataca aagataaaat tcgcgcggcg catcaggatg aaccgcagtt tggcgcgcaa
     7081 agtacgccgc tggatgagcg tacggaacgt ttgatccttg cgctggtctt tgccgctaaa
     7141 agtgatggcc atattgatgc caaagaacgt gcggcaatcg accagcaatt gcgagaagcc
     7201 ggtgtggaag agaaggggcg tgtactcatt gagcaggcaa tcgaacaacc gctggatcca
     7261 caacgcctgg ctaccggggt ccgcaatgaa gaagaggcgc tggaaatcta tttcctgagc
     7321 tgcgcggcta ttgatattga ccattttatg gaacgcagtt acctgaatgc actgggtgat
     7381 gcgctgaaaa tcccccagga tgttcgtgaa ggcattgaac gcgatctcga acagcaaaaa
     7441 cgtactttgg cggagtaatt ccgctctctg gatagcctga agctgattat ttcgggttat
     7501 ccagaaccgc acagccattt gagcaggctg attttctctg tctgcataac attcgatatc
     7561 cgcgccatta cctgtcatca tctaagcaat gactaccctg tttcgcttgc atccccggtg
     7621 agttttgcca cccttataag atgtttcaac cagaaagaac aataacatgc taccaaaagc
     7681 cgcccgcatt ccccacgcca tgacgcttca tggcgatacg cgcatcgata attactactg
     7741 gctgcgggac gatacgcgtt ctcagccgaa agtcctggac tatctgcaac aagaaaatag
     7801 ttacggtcat cgggtgatgg cctcgcaaca agccttgcag gatcgcatct taaaggaaat
     7861 catcgaccgc attccgcaac gagaagtttc tgcgccctac atcaaaaatg gctaccgcta
     7921 tcggcatatt tatgaaccag gctgtgaata tgctatctac cagcgtcaat cggcgttcag
     7981 tgaagagtgg gacgagtggg aaacattgct cgatgccaac aagcgcgcgg ctcatagtga
     8041 gttttattcg atgggcggaa tggcgattac gccagataac accattatgg cactggcaga
     8101 agattttctt tcccgacggc agtatggcat tcgttttcgt aatctggaaa caggtaactg
     8161 gtacccggaa ctgctggata acgttgaacc cagctttgtc tgggcaaatg actcctggac
     8221 tttctactat gttcgcaagc atccagtgac gctgctgcct tatcaggtct ggcgtcacgc
     8281 catcggtacg ccagcatcgc aagataaact gatttacgaa gaaaaagacg atacctatta
     8341 cgtcagcctg cataaaacga cctcgaagca ttatgtggtc attcatctgg ccagcgccac
     8401 caccagtgaa gttcgcctgc tggacgcgga aatggccgat gccgagccgt ttgtttttct
     8461 gcctcgccgc aaagatcacg aatacagcct tgatcactac cagcatcggt tttatctgcg
     8521 ttccaaccgc cacggtaaaa actttggctt ataccgtacc cgtatgcgtg atgagcaaca
     8581 gtgggaagag ttaattccgc cacgtgataa cattatgctg gaagggttta cgctgtttac
     8641 cgactggctg gtggttgaag agcgtcagcg cgggttaacc agtttgcgcc aaatcaaccg
     8701 caagacccgg gaagtcattg gcattgcctt tgatgatccg gcctatgtga cctggattgc
     8761 ctacaatcca gaacctgaaa ccgcgcgatt gcgttatggt tattcttcca tgaccacacc
     8821 agatactttg tttgaactgg atatggatac cggtgagcgt cgtgtattaa aacaaacgga
     8881 agttcctggt tttgatgcgg cgaattaccg cagtgaacac ctgtggatag tcgcccgtga
     8941 tggcgtcgaa gttccggttt cgctggtcta tcatcgcaaa cattttcgca aaggacacaa
     9001 cccgctgctg gtgtatggct acggttctta cggcgcaagt attgatgccg atttcagttt
     9061 tagccgcttg agtttgttag atcgtggctt tgtctacgcc attgtccatg ttcgcggcgg
     9121 tggtgagctg gggcaacaat ggtacgaaga cggtaaattt ctgaagaaga aaaatacgtt
     9181 taatgattat cttgatgcct gcgatgcatt gttaaaactg ggctatggct ctccttcgct
     9241 ctgttatgcg atgggcggaa gtgcgggggg catgttgatg ggcgttgcaa ttaatcaacg
     9301 cccggaatta ttccacggcg ttatcgccca ggtaccgttt gttgatgttg taacaacaat
     9361 gcttgatgaa tcaattcctc ttaccactgg tgagtttgaa gagtggggga atccgcagga
     9421 tccgcaatat tacgagtaca tgaaaagcta cagcccatat gacaacgtca ccgcacaggc
     9481 ttatccgcat ttactggtaa cgaccggttt gcacgattct caggtgcaat attgggaacc
     9541 ggcaaaatgg gtcgctaaat tgcgcgagct gaaaaccgat gaccatcttt tattgctctg
     9601 taccgacatg gactcaggtc atggcggtaa atctggtcgc tttaaatcgt acgaaggcgt
     9661 agcgatggaa tatgcttttc tggtcgcgct ggcgcaggga acattacccg ctacgcctga
     9721 ggattaagta ttttccagat aatgtttcag tgttaaacgc agctccgggc tcatgctgtc
     9781 caggttatta aacaaccagc gcagatagcc cggatcgcgt tcggcaacgt cggaaaccgc
     9841 tttgccacgg tatttgccaa aggtgaaggt cgtcatcaac gacggacgtc cggtgatatc
     9901 ggccatctgt tctgccgtcc agccggaggt gttcataata tcgataagca acgcagcggt
     9961 gatataacag tcatacaacg cgcggtgatg atgcaggccc ggcggcgtct gtacattgag
    10021 cttccgtgtt ttatataacg ccatattgct gtacttgatc cctggccaca aacgacgggc
    10081 cagtttcata gtgcaaatcc actcaccggg catctcaggc agtacgcggc ggtcaaagct
    10141 ggcgttgtgc gcgacatacc attcactacc gtaatagtgt gggatcacat cttcaatcca
    10201 cggtttatcg gcgaccatgg cttcggtgat gcgatgaatc gccatcgctt gtggactaat
    10261 aggacgatcg gggcgcacca ggtggctcat ggggttgacg atttttccgt caatgacatc
    10321 aacagaggca atctcaacga tccctccctg caaaccgcag gtttctgtat cgataatgcg
    10381 caacatggca tgctccaggc cgaaaaagcc tagcgtaatg gaatgatatc gccttgccaa
    10441 ccctgtgatg aacgctgacc gactaacaat aatgaaccac gatcggcgcg aacgatgaga
    10501 cgaccatgtt catcccatag tgcactgtta ccacgggcat ttgccatcag cacggcaatt
    10561 gagtactgat gtgaaaaaaa ttgtaagcgg cgggtagatg ccagtaggtc gggttccccc
    10621 agacattgcc ctgtcgtaaa cagtgaacag gtcgggtcca tatccatacc ttccggttgt
    10681 tcatcgacca ccgtgatggt tctggaacga cggcccagac atgcaccatg actctgatgg
    10741 tagatccctg gtgtttttcg ccacggtgcg aacaccgcaa ttccacgaat aaagcgatcg
    10801 ttatattcaa cgggaaggcc ggcaatgatg gtcattcgcc aggtcgttgc agcataacaa
    10861 agcgggtcta acagcgagag atcgggtggg gcaggaaggg cacgtcgcga ataatcacac
    10921 cccagtaaag aaagcgatgg aaaaactaat agctgacatt gttgcctggc ggcggcactc
    10981 acaaactcca gatgatgagc tacctgttcg gtcagtgagg ttttacgagg ctcatattgc
    11041 gctgctgcaa ctttccagaa cgacataatg acttcctttt caaaatgact gcaaattatg
    11101 ggtgcggttt agaaaatact cataatttag caggcgttat gtaagaaagt gtaactctgt
    11161 ataagtttta tttaagtttg ggctcgtaag gtaaacgtga cagattgacc gaagccaaac
    11221 ggtgggcgat aagccgctcg cgaaaccagc tgcgcaaatg ttcaggctgt tcacgttcaa
    11281 ccgcttcagc gatcaccggc atattgtagc gttctttaaa tgccaccccg gccgccgcca
    11341 aatcgacatt cactttatcc atttctgttt gatccagttt agccagattc ttcagcattc
    11401 ttaatctcct catcattcgc gggcaaaagt tactaagagt gagcctggat ggcaagccgg
    11461 aattgatgat cctcgactca gacattagac aaggttatgc tctgtaaaaa agttattaca
    11521 aaaaggaata gataatatgg cttcaactgc acgctccctt cgctacgcgc tggcaatcct
    11581 gaccacttcg ttggtgacac cttccgtctg ggcacatgcg catttaacgc atcagtatcc
    11641 tgcggcaaat gcgcaagtga cagctgcccc gcaggcaatc actttaaact tctcggaagg
    11701 cgttgaaaca ggattcagtg gtgcaaaaat cacggggcca aaaaacgaaa atattaaaac
    11761 attaccggcg aagcggaatg agcaggacca aaagcaactg attgttccac tggccgattc
    11821 gctgaaaccc ggaacctaca ccgttgactg gcatgttgtt tcggtggacg ggcataaaac
    11881 gaaaggacac tacaccttta gcgtgaaata aatcatgctg gcgtttacct ggatcgcgct
    11941 gcgatttatc catttcacct cgttgatgct ggtttttggc ttcgcaatgt acggtgccta
    12001 gctggccccc ttaacgattc gtcgcttgct gacgaagcgt tttctgcgtt tacaacagca
    12061 cgctgccgtc tggagtttga tcagtgccac tgcaatgctc gccgttcagg gcgggttgat
    12121 gggaacggga tggtcagatg tattttcgcc aaacatttgg ctggctgttt tgcaaacgca
    12181 gtttggtggc atctggttat ggcaaattgt tctcgccctc gtaacgttga tcgttgccct
    12241 tatgcaaccg cagaatatgc cacgtttgct atttatgctc actaccgcgc aatttattct
    12301 gctggcggga gtcgggcatg cgacgctgaa tgaaggggta acggcgaaaa tccatcagac
    12361 taatcacgca attcacctga tctgtgcggc ggcgtggttt gggggattac tgccggtgct
    12421 ctggtgtatg caactcatca aaggccgttg gcgacaccag gctattcagg cgctgatgcg
    12481 tttttcctgg tgcgggcatt ttgcggtgat cggcgtactg gcaagcggcg tgcttaatgc
    12541 attgttgatt accggatttc cccccacgct tacgacttac tggggccaac tcttaatgct
    12601 caaagccatc ctggtgatga ttatggtggt catcgcgctg gctaatcgtt atgttcttgt
    12661 accgcgcatg aggcaggatg aagatcgtgc ggctccctgg tttgtgtgga tgactaaact
    12721 cgaatgggcg ataggtgcgg tagtgctggt gatcatcagc ctgcttgcga ctctcgaacc
    12781 tttttgatgg attacgaaaa tgatgaaaaa aagtattctg gcgtttctgt tactcaccag
    12841 ttctgcagcg gcgctggcgg caccgcaggt gattaccgtc agccgttttg aagtgggtaa
    12901 agacaaatgg gcgtttaatc gcgaagaggt gatgctgact tgccgaccgg gtaatgcttt
    12961 gtatgtcatc aacccaagta ccctcgtgca gtatccttta aacgatatcg cacaaaagga
    13021 agttgccagt gggaagacta aagcccaacc catttcggtg attcagattg atgatcctaa
    13081 caatcccggc gaaaaaatga gtctggcacc gtttatagag cgagctgaaa aactctgtta
    13141 attacctaaa atagcctttt gatttccaat aaaaaaccgc ctcagttctt tcaccagaac
    13201 gggcggtttt taacatttaa gctgatgacc accacgcttt ttattgacca ttttgcacgc
    13261 aaactggaaa acctggcgtc gtcatctatt cttaaagagc aaggcaacta agcctgcatt
    13321 aatgccaact tttagcgcac ggctctctcc caagagccat ttccctggac cgaatacagg
    13381 aatcgtgttc ggtctctttt tatctgttaa aagccagaag cattcccttc gctgacttta
    13441 tagtcaacca taacacacac tctactgtct gcgtccagcg ttttttaaca ttcttgttaa
    13501 gattatgtga tctttagcgc gggaggaaaa tattgatgaa acagcctgcg cccgtttatc
    13561 agagaattgc gggtcatcaa tggcgacata tctggctttc aggcgatata cacggttgtc
    13621 ttgagcagtt gcgccgcaaa ttatggcatt gtcgttttga tccgtggcga gatttactta
    13681 tctcagtggg agacgttatc gatcgtgggc cgcaaagttt acgttgcctg cagttactgg
    13741 aacaacattg ggttcgtgcg gtaagaggca atcatgaaca gatggcgatg gatgcgctgg
    13801 catcccagca gatgtctttg tggttcatga atggcggcga ctggtttatt gcgctggcag
    13861 ataatcaaca gaaacaagcg aaaacggcgc tggaaaaatg tcagcatttg ccctttattc
    13921 ttgaagtaca cagtcgcacc ggcaaacatg ttattgccca tgccgattat ccggatgatg
    13981 tttatgaatg gcaaaaggac gttgatttgc atcaggtctt gtggagccgc tcgcgattag
    14041 gtgaacgccc aaaagggcag ggaattaaag gtgctgatca tttctggttt ggtcatacac
    14101 cgttgcgaca tcgcgtggat attggcaacc tgcattatat tgataccggt gctgtctttg
    14161 ggggcgaact gactcttgtg caattgcaat aattaaaaat caccgtactc ctgtgctggt
    14221 cgccagaaac catcaataaa atcctcgatt ggaaaacaac cgccatggcg gatccgttga
    14281 tcgctcatag aataaagaca ctgctgttcc gtgttgtaga catccacaac aatatcttca
    14341 caaccgccat ccaggtagca aacaaaaagt accagcgcga acatttcatc cccgaagtgt
    14401 ggtgccgtac cgttaagttt aggagagatt ttacaacggg gaaataacca ggacaaataa
    14461 cccgccatgc cgacgggttc tttttggatc aggcaagacg cataatccag gcatcagatt
    14521 gttgatcgta acgggtacgg taaaggacag agtgttcgcc atttagcagg gcaggaatac
    14581 tggtttcagc atcccaggca tccagttgca tacataaatc ggggtctgat ttacaaggaa
    14641 tggttaacgt tcgatcaccc ggcgattcgc cgtgtaattc gccgtcgtcg atttcaaaag
    14701 cgcctatgcg cacactggtt ttcatcttcg tgctctccat ttagctgccg gttgtggtac
    14761 gaccagtccg atcgcaccat ttttcagcct agttgaggca gccgaatgtg ccagtcaaaa
    14821 agtgcgcttt ttttgtgcgc tgtcaggcgt taccggtaaa aagtttccca tcgcgcacca
    14881 gttcacgcgg gtagctgttt ttcagtcgcg aaccaatccg tttggctaaa ccaatcggct
    14941 gatgctggaa agtaaccaat acatcatccg ctactggcgc ggcttgcggg taaacatcgc
    15001 gcccgcgata ccactcttcc gcttcctgcg gtgtcagttc aaaagcgttc acattgtcgg
    15061 gggaggcaag ggcaataacg gcttcatgct gccagcgata acctttgttg tgcgtttcgg
    15121 caagtttaat ccccaagcga gaaaatcgga ctttaccgat cagggcttca atgcccaccg
    15181 ggaacaacca cagttctttg tcacgctgcc agagtcgcag gttttcatcc cagtttaagc
    15241 caacacttgc agccgcctga cgaatttgtc cggcttcgcg atctttcacc gggctgaacg
    15301 ggaaattgcc cactttgtat tttggggtgg gtaaggcggg aatcgcctga gttttacgca
    15361 gacgagcaac gaagaagcct tcgcagtcgt aaatttgtgg gaaaacatgc aaaaagcctt
    15421 cctcggtcag cgccttgttt gcaccaggga agagatcgcc aagcggtaaa aactctactg
    15481 cgtcgggata agtctctttc agccacaggc aaacggcttc gttttcttcc cggtttaagg
    15541 tacaggtcga gtaaaccagc gtaccgccag gacgtaatgc atgaaaggcg ctgtcgatca
    15601 gttcccgttg ggtcgctgcg atttcctgat tgctttctgg tgaccagttt tttagcgcat
    15661 cgggatcttt acgcaccacg ccttcgccgg agcagggagc gtccagcaaa atggcatcga
    15721 acatttctgg cactgccgca ccaaatacgc ggccatcaaa atgcgtgagc gcaacattac
    15781 tgatgccaca gcggctgata ttggcatgta acacttttac ccgactggcg gaaaactcat
    15841 tggcaaggat tgccccttcg ttattcatcc gcgcggcaat ttgcgtcgtt ttggagccgg
    15901 gcgcggcagc gacatccatc acccgctgtg gtgcattacc gtcagcaaac aaggcggcaa
    15961 caggcaacat tgaactggct tcctgaatat aaaacaggcc gcttaaatgc tcggcggtac
    16021 tacccaacgg caatgcatct tcactgtcgc gttcaatcca gaaaccttct tcacaccacg
    16081 gaattggcgt aagcgtccag ccataaggag cagttaattg caggaaatca gcaacggagg
    16141 ttttcagcgt attaacgcga atgctgcggc gcaacgggcg ctgacaggcg gcaagaaaat
    16201 catcaaaaga gagcgtcgaa ggcatcgctt cgcgcatttg tgtcagaaag gcgtccggga
    16261 aataaacggt gtgttgggcc acgagcatat accactggca aaaaaacagg cgcgcagttt
    16321 agcataaacg ctccggcgca ggcacgccgg agcagtgggc attatttggg aagcgcagtt
    16381 ccccattcac gccactcttt cggttcactt tcctgcaaca ggaagtgctt gccttcctgg
    16441 gctttcggtg ccagtggcgt acccggaggc gtggcgaagg cgatgccgcc acggataaac
    16501 tggttaaagg tgccggtttt cactacgccg cccgtcagac caaagtccag actgtaaccc
    16561 gatgccaacc agaagacgga attgttacgc accaggtgtt gatagcgttt actgatgcgc
    16621 atcgcaatca tcacgcgatc tgacaatgtc cccagcgtca ttccggtaac cgtaccgact
    16681 tccagaccac ggaacagcac aggcgtaccg atgcctaacg aaccggcttc cggcgcttca
    16741 acaataatgc ttaagccatc caggtaacgc gaatcagtta tggtagcctc ttgtaattca
    16801 aagtcgcgcc gaggattgcc ccggcctggt tcgacgttga tatacggctg gaggatagta
    16861 tcaagatgct caacgccagc tgccgaaatt tgcggtgtga ccactgagaa gcgtgtacca
    16921 ccgcgtgcaa aggtctggac atattccggg taaagtaccg cctttgcctg tacttcattg
    16981 cgcgcggtaa tcagatccag cgtctggatt tgcccgatat caataccgag atagcgaatt
    17041 ggcatcccga ccgccagttt tccggcatcg aaagcgtgaa gcgtaatttg cccgccaacc
    17101 gcacgggccg ctgtttcgga agcatacaga atacgcttgt cgcctttacg ctgactggcg
    17161 ctggcaccgc tgaggttatc gaagctaata gcccccttta atgctctgga gagcggggat
    17221 gcctgtacgg tcagaccact accatttagc tgaactttcg ccccaccttc tgcccagaac
    17281 acgctgttgc tggtcagaag gttgcgatac tccggcttaa tatgcagatc gatatcaaac
    17341 gcgttagctc gcggacgcac agtaataact tcaccaactt caaatttacg gtacagcact
    17401 accgatcctg cctgcacatc cggcagcgtc tctgcactca aacttacggt tgtggtgggt
    17461 aaatcgctaa ggctgttctc cagcgctttt tccagattgg catacagtgg atagctggct
    17521 ttcatctcgc ctttatcgcc cggcagaata cgtatcccgc cgttaatcca ttctgaggcg
    17581 ctggcaccga gaaactcaac gccatccagc cccaccttca cgtcgacacg gctgttgacg
    17641 acaaatttgc tatcgccttt taccagttcg cgatgctgag gctcgatggc gacggtaaag
    17701 gtaacgcctt tgctggtgag tttacgatca ataacctggc ctacttgcac accgtggaga
    17761 atgagcggct gaccagcatc aataccgtaa ctttctggtg ctgtcagggt cagtgtcaga
    17821 acatccggct cttgtagcag ggctttttcg cctggcacaa caacgaactc tttgcgtggc
    17881 tcaccatcgc cgggcaccaa ctcaaaggtt tttccggtca gcagggcgct gagattggca
    17941 tcgctaaggg ataatttcgg attacgtaat tcgatgcggg tattttcccg taacagcgta
    18001 acgacgctgg gatcaacggt catttctccg gtgactttac caccaggatt taaatccagt
    18061 ttagtcagct gtccgacttc cagcccctga tacattaacg gcgtcgagtc ggcggttaat
    18121 ccggccccac tcggcagttc cagttttatt attacgccac gctggctgtg ggcgagatct
    18181 tcatacagac caaaggtatc ttccgcctcg gcaggtttcg actcttcagg tgaatcgaag
    18241 gcaatcgcac cgttaaccag tgccgccaga ctttccagtt tcaccttcgc gccactgata
    18301 ctgacgttgg catcaacgcc ggaaacgttc cagaaacggc tacctttttt caccaggtcg
    18361 gtaaaacgcc gctcgatcag gacatcaatc accacgcctt gcttattggg attgatggca
    18421 tagtcgtaga ctttacccac cgggatctta cggaaataga ccaatgaacc gctgttcagt
    18481 gaaccgagat cgggggcttg caggtggatc atcagatcgc cattatccag ccgatacttc
    18541 ggttgggtat cgagtgcgac aaagtgatcc tgctctttac ctttacctgg catcatgccg
    18601 atatagttac caccgacgag ggcatccagc ccggagacac ctgccagtga ggcctttggc
    18661 gtcaccagcc agaactgtgt ctcttcgcgc agcgcatctt tcatatcaga cttgatgctg
    18721 accttgactt caatcttacg aagatcgtcg ctcaggctga tatcctgcac agttccgact
    18781 tcaacgccct gataacgaac aggggtgcgg cccgggacaa taccatccgc cgacataaag
    18841 tcgatggtga cggtattacc ccggtcctga taactatccc aaatcagcca accggcaatc
    18901 attagcgcga tgaacggcag cagccagaaa ggtgagatac ggcgtttatt tttaatctgc
    18961 gcttcagtcg tcgaagcggg cgtttcctga ctcatgtgca tcccaaagta agcggctgtc
    19021 cagccattcc acagcaagaa tagtcaaaat taccgctgcg ccgaaataaa acgcagccgg
    19081 tcccatagta aaagcgagga tctgatcgcg attaatcagc gacatggtta aagatatgac
    19141 aaacaggtcg agcatcgacc agcgaccaat ccaggtcacc atccgcagta acagaatgcg
    19201 tgtgcgtaaa ccttgctggc atttaaaatg aatactcaac agtaaagtga acatgacgat
    19261 cactttagta aacggtacca gaatactggc gataaacacg attcccgcaa cggcaatgtt
    19321 gctacttgcc agcgacataa tcccggaaag aatcgtatct tcctgccggc caccattcag
    19381 ataaatgata gaaataggca acaggttggc aggtaacaac aaaacgattg acgctaacag
    19441 cgccgcccag catttttgca gactatgacg gcgacgcagg cgtagcggga tatggcaacg
    19501 cgggcagcga ccacgctgat caggatagcc ggtaaaatgg cacccaagac agacacggag
    19561 tttctcgtct ctacgcgtag tggggcgctg tggataaaat cgctcccaca gttcttcgac
    19621 attgagatgt gacaacgtca ccgtcgttaa aatcaccaac gccacaaaag agaacaagcc
    19681 gacacccgcc tggatatggg cataatcttg tacctttata gaagcaacgc caatgccgac
    19741 caggtagata tccagcatta cccactcttt aagtcgctca agcatcagca gtaccgggcg
    19801 tagattcatt cccagtcggt taccaaacca taaataagct atggaggtca ccagaatgag
    19861 gggggcacca ataacgcaaa aaaagaccat tgatcccgtt atcgcatcgc cctgtttggt
    19921 catttgccag atgccttgca tgacgttggc gtcaatacga atgcctaaca gccagatatg
    19981 caacagcggt tcgccccagg caaacggcat cagcaacagc atggtgaagg ccattgccgc
    20041 caggcgcgtt agcgaccagt cgcgcccgtc acgaattttt gcctgacagc gcggacaata
    20101 ggcactttga tgagaattta tctcgggcag gctaaacagc atgtcacatt gcgggcaacg
    20161 ttggtaatca ccacgcggca gttcctcacc gatagccctc actgttatct tttttgtcgg
    20221 cgtaatttgt ggtgtgttaa gagccatgac gtgccaaatg taaaaaga
//
LOCUS       DAZGAP010000095        20118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGAP010000095 DAZGAP010000000
VERSION     DAZGAP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.095"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            214..714
                     /gene="tssB"
                     /locus_tag="ACHLAM_002937"
     CDS             214..714
                     /gene="tssB"
                     /locus_tag="ACHLAM_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7854435.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            749..955
                     /locus_tag="ACHLAM_002938"
     CDS             749..955
                     /locus_tag="ACHLAM_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854436.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPY"
     gene            1024..2499
                     /gene="tssC"
                     /locus_tag="ACHLAM_002939"
     CDS             1024..2499
                     /gene="tssC"
                     /locus_tag="ACHLAM_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7854437.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2506..2919
                     /gene="tssE"
                     /locus_tag="ACHLAM_002940"
     CDS             2506..2919
                     /gene="tssE"
                     /locus_tag="ACHLAM_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7854438.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2923..4773
                     /gene="tssF"
                     /locus_tag="ACHLAM_002941"
     CDS             2923..4773
                     /gene="tssF"
                     /locus_tag="ACHLAM_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7854439.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSHPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4737..5819
                     /gene="tssG"
                     /locus_tag="ACHLAM_002942"
     CDS             4737..5819
                     /gene="tssG"
                     /locus_tag="ACHLAM_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7854440.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5844..7124
                     /gene="tagH"
                     /locus_tag="ACHLAM_002943"
     CDS             5844..7124
                     /gene="tagH"
                     /locus_tag="ACHLAM_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO7854441.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCVFTQQGNVGHGPECQWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITAGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7121..7645
                     /gene="tssJ"
                     /locus_tag="ACHLAM_002944"
     CDS             7121..7645
                     /gene="tssJ"
                     /locus_tag="ACHLAM_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7854442.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7648..8979
                     /gene="tssK"
                     /locus_tag="ACHLAM_002945"
     CDS             7648..8979
                     /gene="tssK"
                     /locus_tag="ACHLAM_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7854443.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGILPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWAEIAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8984..9745
                     /gene="icmH"
                     /locus_tag="ACHLAM_002946"
     CDS             8984..9745
                     /gene="icmH"
                     /locus_tag="ACHLAM_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO7854444.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FYGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9754..12543
                     /gene="tssH"
                     /locus_tag="ACHLAM_002947"
                     /gene_synonym="clpV"
     CDS             9754..12543
                     /gene="tssH"
                     /locus_tag="ACHLAM_002947"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO7854445.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEINLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQALVQEIIALRQQLLGVAEDD
                     AAPLPDADTVEDTPPESESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRL
                     AQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGD
                     TIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGR
                     QYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHP
                     DVLNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPV
                     LADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDE
                     IMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVE
                     DAQNDESVTKDETVL"
     gene            12540..13283
                     /gene="vasI"
                     /locus_tag="ACHLAM_002948"
     CDS             12540..13283
                     /gene="vasI"
                     /locus_tag="ACHLAM_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO7854446.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13288..14700
                     /gene="tssA"
                     /locus_tag="ACHLAM_002949"
     CDS             13288..14700
                     /gene="tssA"
                     /locus_tag="ACHLAM_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7854447.1"
                     /translation="MAMNLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQHDALSGELAKLAQCWQSYN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAT
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14809..18243
                     /gene="tssM"
                     /locus_tag="ACHLAM_002950"
     CDS             14809..18243
                     /gene="tssM"
                     /locus_tag="ACHLAM_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO7854448.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGIYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     GLFPQALLAEPNLATESRTWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPLGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKL
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18254..19664
                     /locus_tag="ACHLAM_002951"
                     /pseudo
     CDS             18254..19664
                     /locus_tag="ACHLAM_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19630..20109
                     /locus_tag="ACHLAM_002952"
     CDS             19630..20109
                     /locus_tag="ACHLAM_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7854449.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELYYK
                     IKLTGAKISDIHVSISHIVVDNSIQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
BASE COUNT         4740 a         5510 c         5554 g         4314 t
ORIGIN      
        1 aggttcacac aatctattta acgcttgcag aatacccctc tttacgatcg ataagaagtc
       61 cttttcttat ttattttgaa gtgtagaatg cggggtgatg aaggagtggt aattttattt
      121 tcaccgccat tagggcggca ttgccgatat ttttttgcgg tacaaatatg ccgtgttcat
      181 ggcctgtacg agataacatg acggatcact tttatgagca aaaaatttga aggcagcgtc
      241 gcgcctcgcg agcgtataaa tatatcctat gtcccgaaga cggacggcca gacggctgaa
      301 gtagagcttc cgcttaacat gctcgttgtc ggcgataccg gaaatacgca ggagacatcc
      361 ccactggatg aacgtcaggc agtttccgtt aacaagcaca atttcggtgc agtaatggct
      421 gaagcagcca ttggcctcaa ttttaccgtc cccgccacgc tgaagggaag cacaacggac
      481 gatgaactga atgtggcgct gaatatcaag tcactggatg atttttcacc ggacagcgtg
      541 gcccggcagg ttccggaagt gaataaattg ctggaactgc gtgaagcact gactgcgctg
      601 aaggggccga tgggcaacct tccggcattc cgtacccagc tccaggcatt gctggaaaat
      661 gaggagtccc gcgagcagtt gcttaaagaa atcggccagg tgagtgggaa ataaccacca
      721 ctatttcatt aaacagggtt atccagacat ggcggcacac aaaaatatct ccgggaaaca
      781 ccccctctct gacgaagaca ttttgcaggc ggtttaccgg gtatgtttca gcccgtccgg
      841 tgaaatcaac cgggatggcg tggatgcgct gattacaaca ctggatcgcc gtatcagtga
      901 tctattggat gagattatcc atctttgtga gtttcagcag agcgcgccgt actgacggcg
      961 cgtacttcac tgacggcgaa aacccggttt cagaaacaca gctaaaacag aaggattttc
     1021 agtatgtctt tacaggaaga ggaactcgtt tccagtcatg ccgggcagac tgatcaggcg
     1081 tcgtcgctgc ttgatcaaat catggcacag acccgtattc agcctggttc ggaaggttat
     1141 gacgttgccc gccagggtgt gacagccttt attgccagca tcctgcaatc cactgcatct
     1201 gccgaaccgg tcaataaact ggccgtggac agcatgattg cagatataga tgaacgcatc
     1261 agccgccaga tggatgtcat catccatgcg cctgcctttc agcaggtcga gtcgttctgg
     1321 cgctcactga aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa ggtcaacgtg
     1381 cttcatgtca cgaagcagga acttctggaa gactttgaat ttgccccaga aattattcag
     1441 tccggatttt ataaacacgt ttattcgtcc ggattcggac agtttggtgg tgaacccatc
     1501 gctgccgtgc tgggcgctta tgagttcaaa aataccgctc ctgacatgaa actgctgcaa
     1561 tacgtcagtg ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt ttccccggag
     1621 tttatggggc tgaactcgtg gaccgaactg cctaatatca aagatcttta tgccatcttt
     1681 gaaggtccgg cctacaccaa atggcgcgcc ctgcgtgact cggaagattc gcgctattta
     1741 gggcttacgg caccccgctt cctgcttcgc cagccctatt caccaacaga taaccctgtt
     1801 aagaacttta actattacga agatgtcagc cagaaccacg aagattatct gtggggtaat
     1861 acggcctgga tgctggcatg caatatcgca gacagttttg ccaaataccg ctggtgtcca
     1921 aacattatcg gtccgcaaag cggcggcgca gtaaaagatc tgccggtgca tctgttcgaa
     1981 acgatggggc agattcaggc caaaatccca accgaggtgc tggtcaccga tcgccgcgaa
     2041 tttgaactgg ctgaagaggg ttttatcacc cttaccatgc gtaaagactc tgataatgca
     2101 gcctttttct ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa agacgcagaa
     2161 accaactata agctgggtac gcagcttcct tatctcttca tcatcaatcg gttagcgcac
     2221 tacatcaagg tgttgcagcg tgaacaactg gggtcatgga aagaaaggag cgacttagaa
     2281 agagagctta atacctggat ccgtcagtat gttgccgacc aggaaaaccc gcctgcagac
     2341 gtgcgcagcc gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt agaaggcgaa
     2401 cccggctggt accaggttgc gctaagcgtg aggcctcatt ttaaatttat gggggcaaat
     2461 tttgagcttt ccctggttgg ccggttagac agggagtaac acaccatgat accggatcgg
     2521 gaaagaacca caggttctct ttttgagcgt atggaagcct cttcagccag aaaccggcag
     2581 ggaggcagta ttcattcgct caggcaatcc attcgccaga acctacgaaa cattcttaat
     2641 acccggtccg gtagttgccg gggtgcgcct gagctgggca ttgatgaacc ggaaggggct
     2701 gaaaacttcc gcgaatcaat gagtcgggcc atcgagcaat gtatagaacg ctatgaaccc
     2761 cggatctcac acgctgaagt gcaggcagtg gtttcatccg cctccagccc gctggatatg
     2821 acgtttcata ttacagcctg ggtgacattt aacgaaacgc atgaggtgct tgaatttgat
     2881 atggcaccga atggcagcca gcattaccgg gtggactgac aaatggaatt tgaagaacgc
     2941 tatttccggg aagaactcga ttacctgcgc cagcttagca agctgctggc aacggaaaaa
     3001 ccccatctgg cccgcttcct ggccgaaaaa gatgcagatc cggatattga acgcctgctg
     3061 gaaggggtgg cttttcttac cggcaatctc cgccagaaaa ttgaggatga attcccagaa
     3121 ctgacgcacg ggcttattaa gatgctctgg cctaattacc tgcgtccggt tccggcaatg
     3181 acccttattg aatatacgcc ggatatggat aagtcttctg taccggtgtt aatcccccgt
     3241 aatgagcagt ttacaaccaa cgccggggaa atcagagttg atgaagtgct gccctctgat
     3301 gctaaaaagg aggagcctcc tccctgtacc ttcaccctct gccgggatat ctggctgctg
     3361 cccgttcgcc tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt tatcaacatc
     3421 accttttcgg tcgcaccggg aacggacttc cgcacgctgg atctgaacaa acttcgcttc
     3481 tggctcggca atgacgacaa ctatacccgt gaccagcttt atttatggtt ctgcgaatac
     3541 ttgcagggtg ctgacctgac tgtgggtgaa cagcatcttc gcctgcctga gtttatgcta
     3601 aaagctgtcg gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa cgtccacagc
     3661 ggctaccgga tccttcagga gtatttctgt taccccgatg cgtttctctt ttttgatctt
     3721 tgtggttgtc cggctttgcc tgacggattg caggcggaat tctttaccct gcaactgcgt
     3781 ttttcgcacc cattgcccgt ggacatccgg ttgcgccgcg actccctgcg cctgtattgc
     3841 gcgcctgcca ttaatttatt tatccaccat gcagaagcca tcacgctgga caaccggcgg
     3901 gcagactatc cgctggttcc cagccgccat tatccggaac attacgatgt cttttccgtt
     3961 aacggcgtga taagccaggt gcaggatatg ttcaggaaaa aagatctggg tcgtcctgtt
     4021 tcgacgcagg ccgcgcgcca gtggcctgcc tttgaaagtt tcagccatca gatggaatac
     4081 agccggaagc gggaagtggt gtactggcat caccggacca aaacatccct gttccatcgc
     4141 ggctttgatc atacccttgc ttttatacat gctgatggca gttatccgtc agacgaatcg
     4201 ctgctcagta atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga acttccgtca
     4261 caaattcgtt ccggcgatat caccggcaca accggtaaaa atgcggctgt tgcttcattt
     4321 cgcaacatta cccgcccgac gcaaccgctc tggccggtca ttgatggcag cctgcactgg
     4381 tccctgctct ccgccatgaa cctgaattat ctgtcattac tggatacgga cgcgctgaag
     4441 caggtcatcg ccaactttga tcgccacgca atccatcatc cgcagacggc gcggctgtca
     4501 caacaaaagc tggatgccat tgagcgtctg gagacccgcc ctgtcgatcg cctgtttacg
     4561 ggcattcccg tccgggggct ggcctccacg ctgtatctgc acccggagcc gtttgtctgt
     4621 gaaggggaaa tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct gtacgccagc
     4681 gttaactcat tccacatgct gaccgttgtg aacacagaaa gccaggagac atggaaatgg
     4741 acggaaagaa ccgggcagca tcctcttatc tgagtccggg aaatcccccg gcagataaag
     4801 agcagaatga tccgctggca caggtttttc ataacgcctg ctcttacaat ttttttgcga
     4861 tggcggagct gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa ttatccctgc
     4921 gtgacgatcc ggcacaggaa accctgcgtt tcagcgccga tgccagcctt gccttcccgt
     4981 gcagtgatat cagtgcgctg aaacgggata cgtcaggggc attcaggatg accaccacct
     5041 ttatggggtt acaggggagc cagtcgcctc tccccggtta ttacctcgat cacctggcct
     5101 ggaaagcggt acatgaacaa agtccggtag gtgattttct ggatatgttc agccaccgcc
     5161 tgacccagtt tgtctggcat atctggcgta agtaccgcta tcacatcagt tttcgtaacg
     5221 gcggtgtgga tgcgttctcg cagcgcatgt actcccttgt cgggctgggc caccgccagc
     5281 ttcgcgataa gctggcgatt aatcacagca aaatgctggc gtattccggg atcctggcaa
     5341 atccgggacg atccccggaa ataatctgcg gcctggtgtc ccactgcttt gatttgtcag
     5401 acgtgacgct gcaaaactgg caacgtcgca aggtggatat tgaaccggat cagcaaaaca
     5461 gtctggggag ctattcactt aaaaatggcg aaaaactggc gggaagatcg gtactgggca
     5521 actttgttct gggtacacgg gtgcccgatc tcagcggaaa attccagctc agtattacca
     5581 gtctgacccg aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc ctgccactga
     5641 cgatgtttgt gtccttcatc ctgcgcgatc agcttgcctg ggacttacat ctggggctgg
     5701 cgccggagca ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg ctgggctgga
     5761 ccagcttcct cggcacaccg gaagaacgac cctcagtcac catcagggta cggtcgtaaa
     5821 ttatgataat cacggagcag aatatgccgg aagagaaact acagacgctt tcattgcagg
     5881 tcattaacgg cagtgagctg gaaagcgggc gggcggcgcg ctgtgtgttc acacagcagg
     5941 gaaatgtcgg ccatggcccc gaatgccaat ggtcggtaca ggatcgtcag cagagcattc
     6001 cggctcaggc ttttaccgtt atcctgcacg atggcacatt ttgtctgcgc ccgcagacgg
     6061 cacaactgtg gctgaaccag gcaaaagtca cagcaacatc agacctgata cagctgcgcc
     6121 agggcgatga gatccagata gggcgtctga tggtgagggt tcatctgaac cggggagata
     6181 tcccccatta cgatgaggaa atggccactc ccgaaaccat cgtcaccaat cgcgatatgc
     6241 tcacggatac cctgctgtca acggagggtg cgccacacta tccgggaatg actcaccggc
     6301 atcagcttgc agacaccgtg gtaaacggtt tttctaccga tccactccag gcacttcagt
     6361 ccgaaagcct gattaccgcg ggcgatccgc tttcaggcat tgcggctgtc cggccatcgg
     6421 caccgctgtc cgatccggca agtaatgggg ggatcaatac tccgtttatg gatctgccgc
     6481 ccatttatgc cagccccggc gatcgtaatg atgacgtctc tgtggcagaa atggcgcaac
     6541 gccaccttgc ggtcaccccc ttactgcgcg gtctcggcgg ttcgcttacc gtgagcaatt
     6601 ccgacgatgc ggatgatttt ctggaagagg ccggacgcac gttacaggcc gcaataaaag
     6661 gtctgctcga tttgcagcaa cagcgtaaca gcctctcaga caaacatttg cgcccgctgg
     6721 aagataaccc gctgcgcctg aacatggatt acgccaccgc gctcgacgtg atgtttgccg
     6781 aaggtaaaag tccggtacat ctggcggctc ccgccgctgt cagtgaaagc ctgcgcaatg
     6841 tccgccacca cgaagaagct aaccgggcag cgattgtgga gtcacttcgt gtcctgcttg
     6901 acgctttctc accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc agccatgaac
     6961 tgcgccagcc gctggatgat gccggagcat ggcaaatgta cagccattat tacgaagaac
     7021 tggcctccga tcgccagcag gggtttgaga tgctgtttaa cgaggtctac gcccaggtct
     7081 atgaccgggt gcttcgtgaa aaacagcggg agccggaagc atgaacagga aggcgtttct
     7141 ggcctgcgtg ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa aaatcagcga
     7201 ggtgatcaaa aacccggata tccaggtcgg gagcctgaag gagcaacctt cggaaataac
     7261 cgtcaccctg ctgaccgaac cggacaccaa taccaatgcg gagggtgaat cagccgccgt
     7321 ggatgtgcaa ctggtgtacc tgactgacga ctcaaaattg ctggcagccg actatgacca
     7381 gatagccagt acgccgctac cggatgtgtt gggtaaaaac tatatcgacc accaggattt
     7441 caacctgttg ccggacacca tcaaaaccct gccgccggta aagctggatg agaaaacgca
     7501 gtttattggt gtcgtggcct atttctccga tgaccaggcc actgaatgga aacaaatcga
     7561 gacggtggaa ggaaccggcc accattaccg cctgctggtg catgtacgtc aaagcagcat
     7621 cgaaatgaaa aaagaggatg aataattatg gcaaccaccc gcaacaaggt gatgtggcag
     7681 gaagggatgc tgatgcgccc acaccatttc cagcaacagc agcgttacaa cgactacctg
     7741 gataaccagc gtttccgggc catgaatgat ttatcctggg gatttaccga actcaccctc
     7801 aacaatgaac tgctggcgca gggtaagatc atgattgaca gcgcgtcagg catactgccc
     7861 gacggcaccg tcttttctat ccccgaccag gacgcactgc ccgatccgct gcacccgcaa
     7921 aattttccgg acgaaagaag ccgcaatatt taccttgctc tgccggtcgc cagtgatgtg
     7981 agaaatgaaa tcagcgacgg gcggcgaatc gggcgttacc ggctgaatta tgccgatgtc
     8041 cgggatctgc attcagaaga aggtgacgcg cgaacactga cgctgggtca actgacgccg
     8101 cgcattatga gcggtgcaga agatatgagc gcctatatta cgctcccgct ttgccgtatc
     8161 agcgatcgcc atgccgacgg ctccctgacg ctggatgacg attttatccc ctcctgccag
     8221 aatattcagg tcagtaagaa actgcgtgtt tatctcaaag aggtacaagg ggccattggc
     8281 ggacgggcaa gcgatctggc aaaccgcatt ggctctccgg cgcagagcgg catcgcggat
     8341 gtggcggaat ttatgatgtt gcagttactt aaccgtaacc agacccggtt tacccatcgc
     8401 gctcgtcgat cccagctcca cccggaagat ttctaccttg atcttgccgg gttgctgggt
     8461 gaactgatga cctttaccga gccgtcgcgc ctgccctgcc cgcttgatgt gtatgatcat
     8521 catgacctga ccaaaacatt taaaacgctg ttaccggaag tcaaacgggc gctgcatacc
     8581 gtactgtcgc caagagccgt caatctgccg ctgcatctgc gtgacggtat ctggcaggcc
     8641 gatattcatg acacagaact gctgcaatct gccacctttg tgctggccgt ggcggcaaac
     8701 atgccggtcg atcagatcca gcgccagttt atccagcagt cgaaaatttc ctcgccggaa
     8761 aaaatccgca atatggtcag tgtgcagata cccggcattc cattgcgtgc cctgatggtg
     8821 gctccccgcc agcttcctta ccattccggg ttcagctatt tcgaactcga caaaagcggc
     8881 caggcctggg ctgaaatagc tgccgccggg gccgttgcac tgcatgtttc cggcagtttc
     8941 ccggatctga acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact
     9001 gtctctgcca ccatttaccg gacacgacga aaaaagccag cgcgactacc atctcgcgct
     9061 tcgtggcaac agcctgaatc cgatgatcga cgcggccacg ccgctactcg gtatggtaat
     9121 gcgcctttca accatgaaca gccagacgat gccggagcat ctttttgctc aggtggtgac
     9181 ggatgttcag gccgtggagc aactgcttca ggagcagggc tacgaaccgg gcgtgattat
     9241 ctcgtttcgc tacatcctct gcacgtttat tgatgaagcg gcgctcggta acggctggtc
     9301 gaacaagaac gagtggatca aacagtcatt gctggtccat ttccacaacg aagcctgggg
     9361 tggagagaaa gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga
     9421 tctgctggaa tttctctggc tctgtttttc tctgggattt cgcgggcgct acaaagtggc
     9481 agcacaggat cagggggaat ttgagcagat ctaccgccgc ctgtaccacg tcctgcataa
     9541 actacgcggc gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc agggcgggcg
     9601 ttatcagctc atcagtcgcc tgaccattaa acatattttt tatggtggcg ttgtcgtgct
     9661 ggcgttgttt tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca
     9721 ccagctaaac aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg
     9781 gtaaaacggc ttaacctgtt ctcccgccag gcgctggaga tggccgcctc tgaatgtatg
     9841 agccagcagg cagcggaaat taccgtcagc catgtgctta ttcagatgct cgccatgcca
     9901 cgcagtgacc tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag
     9961 gcgctgacgg tggagaacta cacaactgcc cgttctgcgg acagctaccc ggcgttttcc
    10021 ccgatgctgg ttgagtggct gaaagagggc tggctgctgg cgtcggctga gatgcagcac
    10081 agcgaattgc gtggcggcgt gttactgctg gccctgctgc attcgccgct gcgttatata
    10141 ccgcctgctg ccgcccggct gttgaccggc attaaccgtg accgtctgca acaggacttt
    10201 gtacagtgga cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaaggcgca
    10261 ggcacactga cggacgctgc tgacaccctg cttgcccgct acgccaaaaa catgaccgca
    10321 gacgcccgta acggcaggct tgacccggta ctgtgccgcg accacgaaat caacctgatg
    10381 atcgacattc tctgccgccg ccgcaagaac aacccggtgg tggtgggcga agcaggcgtg
    10441 ggtaaaagtg ccttaatcga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac
    10501 aaactgaaaa acaccgatat catgaccctt gacctgggcg cattgcaggc cggggcgtcg
    10561 gtgaagggcg aattcgaaaa acgcttcaag gggctgatgg cggaggtcat ttcctctccg
    10621 gtgccggtca ttctgtttat tgacgaagca cataccctga ttggcgcggg caaccagcag
    10681 ggcgggctgg atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga actgaaaacc
    10741 atcgccgcca ccacctggag cgaatacaaa aaatacttcg agaaagatgc cgctctgtcg
    10801 cgccgcttcc agttagtgaa ggtcagcgaa cccaacgccg ctgaagccac cattattctg
    10861 cgtggtctgt cggcggtcta tgaacagtct cacggcgtgc tgattgatga tgacgccttg
    10921 caggccgcag caacgctgag cgaacgttat ctctccgggc gtcagttgcc ggacaaagcc
    10981 attgatgtgc tggataccgc ctgcgcccgc gtcgccatta acctttcctc gccgccgaag
    11041 caaatctcgg cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag
    11101 cgcgagcttc gcatcgggct gcgtaccgac acatcacgga tgaccgaggt gctggtgcag
    11161 tatgatgaaa cgctgacggc gctggatgaa ctggaggccg cctggcatca gcagcaggcg
    11221 ctggtacagg agattatcgc gctgcgccag caattgctgg gcgtggcaga ggacgatgcg
    11281 gcgccgttgc cggacgcaga taccgtggag gatacgccgc cagagtcaga gtcagagtca
    11341 gagtcagagt cagagtcaga acaggataat accggtgccg taccggctga tgaggccgac
    11401 agagaacagc cggaagagac cgctgaaaca gtttccccgg tacagcggct ggcccagctc
    11461 actgccgaac tggacgccct gcataacgac cggttgctgg tctccccgca cgtcgataaa
    11521 aaacagattg cggcggtgat tgccgaatgg accggcgtgc cgctcaaccg cctgtcgcag
    11581 aatgaaatgt cggtcatcac cgacctgccg aaatggctgg gcgacaccat caaaggccag
    11641 gacctggcga ttgccagcct gcataagcat ctactgaccg cacgcgcaga cctgcgtcgt
    11701 ccgggacgcc cgctcggtgc gttcctgctg gctggcccca gcggtgtggg taaaaccgaa
    11761 accgtcctgc aactggcaga gctgctctac ggcggtcgcc agtacctgac caccatcaat
    11821 atgtccgaat tccaggagaa acacaccgtc tcgcggctga ttggttcgcc tccgggctac
    11881 gttggctacg gtgaaggcgg cgtactgacc gaagcgattc gccagaaacc ctactcggta
    11941 gtactgctcg atgaagtgga aaaagcgcac ccggatgtgc tcaacctgtt ctaccagtcg
    12001 ttcgataagg gcgaaatggc agacggtgaa ggccgcctga ttgactgcaa aaatatcgtc
    12061 ttcttcctga cgtccaacct cggctaccag gtaatagtcg agcacgccga tgacccggaa
    12121 accatgcagg aagaactgta tccggtgctg gccgacttct tcaaacctgc cctgctggcg
    12181 cgtatggaag tggtgccgta tctgccgctg tcgaaagaga cgctcgccac cattatcgcc
    12241 ggaaaactgg cccgtctgga taacgtgcta cgcagtcgct ttggtgcaga agtggtcatt
    12301 gaaccggaag tgacggacga aatcatgagc cgcgtcaccc gcgcggaaaa cggcgcgagg
    12361 atgctggaat cggtcatcga tggcgacatg ctaccgccgc tctcgctgct gctgttgcag
    12421 aaaatggcgg ctaacacggc gattgcccgg attcggttgt cggcggtgga cgacgcattt
    12481 acggcagacg tggaagatgc tcagaacgac gagtccgtca caaaggatga aacggtttta
    12541 tgagcaggtt gcgcgttttc cggcagacag gatggtttat cgcgggcctg atgacgggct
    12601 tacccgccac tgccgcgccc gcagaaacat cgtcaatggc cggtgtggcg gtcgccgtcg
    12661 ccaccacgcc cccgccggat gcgacagcca cgttacaggc catgcagtcc tgtcggcggg
    12721 agtccgcggc gctggagcgg cttgactgct atgaccgcct gctggcaccg ctgtccccgt
    12781 cagggtttga cggggcgctg gtcaaagccg gttttgtggg cgaggcctgg acccgcgcca
    12841 cagagcagga aaaacgccgc gagggcaaca ccacagaact gctggtgacg caggtgccgg
    12901 gggagcgccc gacggtggtg ataaccaccc cggctatcgg ccatgttccg ccgcgcccgg
    12961 tgctgatgtt cagttgcgtg gacaatatca cccggatgca ggtggcgctg atgcaccctc
    13021 tggatgtcca tgatatcgcc gtcactctga atgccgacag ccgggcattg cgtagccact
    13081 ggtttgtgcg cgagaacggc accctgctgg agtccagccg ggggctgtcc gggattgatg
    13141 aaattaagca actgttcggc gcaaaaacgc tgacagtgga taccggcgca gataacgccg
    13201 caggaaagct gacctttaac attgacgggc tggcaagggc gattgccccc ctgcgcgttg
    13261 cctgccactg ggcgggagaa taaaaatatg gcaatgaact tacgcgatcc gaatgtctgg
    13321 atatcgcacc tgctggaaaa cctgccggaa gaaaaactgg catcggcgct gaaagatgac
    13381 aacccggact gggagtatat cgacggcgaa atcgtcaaac tgggatctct ggcccacagt
    13441 caactggata tcccggaact ccagcgccgg gggctggtca ttctggcttc ggagagtaag
    13501 gactttcgtc tgctggcgca tctgctgaga accctgcaac acgccggtga tcctcatctc
    13561 gcattgcgcc tgctggcgct gtatgtggag cattactggt cggtggctgc gccgcagaat
    13621 atggcacata aaaaacgctt tgccagccag gtcattaaac gctttgaaac aggtattgaa
    13681 ggcttttcac agaacgccgc cacagcgcag cacgacgcgc tgtcagggga actggcgaaa
    13741 ctggcgcagt gctggcagtc atataacgcc ccggaactgg cactggctac cgatgatctt
    13801 tttgccctgt accagcgtgc gtttaatcgt gcggcaccgg ctcctgcccc cactccggcg
    13861 gcctccgata gttcacgaca gaccaccgcc acgtctgaaa gcggcgtgac gcaacccagt
    13921 gctccggctc cccaaatcgc catcgacagt cacgacgaca aagcctggcg cgacacgctg
    13981 ttaaaagtgg cggctatttt atgtgaacgc cagccggact cgccgcaggg ctatcgcctg
    14041 cgccgccatg ccctgtggca atccatcacc agtacacccc aggcggaaag cgatggacgt
    14101 accccattgg ctacggtctc tgccgatatg gtggcggatt accagtcccg gcttgccagc
    14161 gcggatatgg cgctgtggca gcaggttgag aaaagcgtat tgctggctcc ttactggctg
    14221 gacggtcact gtctttctgc acagacggca ctgcgtctgg gttacaaaca ggtggcagac
    14281 accatccgcg atgaggtcat ccgcttcctt gagcgtctgc cccagcttac cgggctgctg
    14341 tttaatgacc gcacaccgtt tctcagtgag cagacgaaac aatggctggc agcttcgccc
    14401 gacggcaaag ttgcaccggt tgcgcaaatc ggtgaggaat cgcaggcagc cagagcctgt
    14461 tttgctgggc agggtctgga ggcggcgctg cgatatctgg acatgctacc cgaaggcgat
    14521 ccccgcgatc agtttcaccg ccagtacctt gccgcacagt tgacggagga ggcggggctg
    14581 atacagcttg cgcagcaaca gtaccggatg ttgttgatga tagggagtca gatgatggtg
    14641 tctgactggg agccatcatt acttacgcag cttgaacaaa aattcacggc agaacaataa
    14701 aagagaacag gagtttttgt gttcagatta ccaacacccc gactactcag cggactcaaa
    14761 tcagccctac gcccggcgat gccccggttt aaagtctccg ctttctggct gctgatactg
    14821 gcgtggattt ttctgctggt atggatctgg tggaaaggcc cgacatggac gctgtatgaa
    14881 gaacagtggc tcaaaccact ggcgaaccgc tggctggcaa cggcggcatg ggggattatc
    14941 gccctagtgt ggcttaccgt ccgggtgatg aagcgcctgc aacagcttga aaaaatgcaa
    15001 aagcagcagc gcgaggaggc cattgatccg ctcagtgtgg agctgaacgc ccagcagcgt
    15061 taccttgacc gctggctgct gcgcctgcaa cgccatctcg acaaccgccg tttcctgtgg
    15121 cagttgccgt ggtatatggt catcggcccg gcgggcagtg gcaaaacgac actgctgcgc
    15181 gagggttttc cgtccgacat tatttatgcc ccggagggcg cacgcggcgc agaacaacgc
    15241 ctgtacctca cgccccatgt cggtaaacag gcggtgatct ttgatatcga cggcacactc
    15301 tgcgctcccg ctgatgcgga tatcctgcat cgccgtctgt gggaacatgc cctcggctgg
    15361 ctgaaagaaa agcgcgcgcg ccagccgctg aacgggatta ttctgacact ggatttaccc
    15421 gatctgctta ccgcagacaa acgccgccgc gagcatctgt tacagacgct gcgcagccgc
    15481 ttgcaggata tacgccagca tcttcactgc cagttaccgg tttacgtggt actcacccgg
    15541 cttgatttat tgcagggttt tgccgccctg ttccagtcac tgaacagaca ggatcgcgat
    15601 gcaattctag gggtcacgtt cacccgccgc gcccatgaaa atgatgactg gcgaacagag
    15661 ttgaatgctt tctggcagac atgggtggat cgaatgaacc tagcgttgcc ggatctgatg
    15721 gtcgctcaga ctcacacccg cgcgtcttta tttagttttt cccgccagat gcagggaagc
    15781 cgtgaaccgc tggtgtcact gcttgagggt ctgcttgacg gcgaaaatat gaacgtgatg
    15841 ctgcgtggta tctatctcac ctcttcgctt caacgcggac agatggatga tattttcacc
    15901 cagtctgccg cccgccagta ccggctgggc aataacccac tggcgtcctg gcccctggtg
    15961 gacaccgcgc cttatttcac ccgtggcctg ttcccgcagg cattactcgc agagcctaat
    16021 ctggcaacag aaagccgcac ctggctgatg cgttcccgtc gccgcctaac ggttttctct
    16081 accacaggag gcgtggcggc actgctgctc atcaccggct ggcatcacta ttacaacggt
    16141 aactatcagt ccggcatcac cgtgcttaag caggcaaaag cctttatgga tgtaccgcct
    16201 ccgctggggg aagatgatta cggcaacctg caactgccgc tgctgaaccc ggtacgcgat
    16261 gccacgctgg cctatggcga ctggggcgac cgcagccgtc tggccgatat gggactgtac
    16321 cagggacggc gtatcgggcc ttatgtggaa caaacctatc tgcaactgct ggagcagcgt
    16381 tacctgcctt cgctgtttaa cgggctggtc aaagcgatga acgccgcgcc gccggagagt
    16441 gaggaaaaac tcgccgtgct gcgtgtgatg cgaatgctgg aggacaaaag cggacgcaat
    16501 aatgaggtgg tgaagcagta tatggcaaaa cgctggagcg aaaaattcca cggccagcgc
    16561 gatatccagg cacaactgat gtcccatctt gactacgcgc tggctcatac tgactggcat
    16621 gcagagcgtc aggcgggcga cggtgacgcc atcagccgct ggacgccata tgacaagctc
    16681 gtggtatcag cacagaaaga actgagcaaa ctgcctgtct accagcgggt ttatcagagc
    16741 ctgaaaacgc gggcgctggg cgttcttcct gctgacctca atctgcgtga ccaggtaggg
    16801 ccaacctttg accaggtgtt tacatctgcc gatgacaaca aactggttgt tccacagttt
    16861 atcacccgct atggcctgca aagctatttt gtaaaacagc gcgatgaact ggttgaactg
    16921 acagcgatgg attcctgggt acttaacctc acccgcagtg tgaaatacag tgacgccgac
    16981 cgtgcggaaa tccagcacca gttgaccgag cagtatatca gcgactacac cgccacctgg
    17041 cgggccggga tggacaatct gaatatccgc aattttgagt ccatcggaca actgaccggg
    17101 gcgctggagc aggttatcag cggcgaccag cctttgcagc gggcgctgac cgtgctgcgt
    17161 gacaacacac agccaggcgt cttttctgaa aaactctctg ccaaagaacg ggaggaagcc
    17221 ctggcagagc cggattacca gttactcacc cgcctcgggc atgaattcgc cccggaaaac
    17281 agtaccctgg cagtacagaa agacaaagaa agcacgatgc aggccgtgta tcagcaactc
    17341 accgagttgc accgctacct gctggcaatc cagaacgcgc ctgtaccagg gaaatcggcg
    17401 ctgaaagccg tgcagttacg gcttgatcag aacagcagcg atccgatatt cgccacccgc
    17461 cagatggcaa aaacgctgcc tgctccgctc aaccgctggg ttggcagact gactgaccag
    17521 gcctggcatg tggtgatggt ggaggctgtt cattatatgg aagtggactg gcgcgacagc
    17581 gtggtgaaac cgtttaacga gcaactggca aataactatc cgtttaatcc gcgttctgca
    17641 caggatgcct cactggatgc cttcgaacgc ttctttaaac cggatggcat actggatacc
    17701 ttctaccagc agaacctgaa gctgtttatc gataatgacc tgagtctgga ggatggcgat
    17761 aacaacgtca ttattcgcga agatattatt gcgcaactgg aaactgcgca gaaaatccgt
    17821 gacatcttct tcagcaaaca gaacggtctg ggaacatcct ttgccgtgga aacggtatcg
    17881 ctttccggca ataaacgccg cagtgtactg aaccttgacg gtcagttagt cgattacagc
    17941 cagggccgta actataccgc ccatctggtc tggcctaaca acatgcgcga aggcaacgaa
    18001 agtaagctga cgctcatcgg caccagcggc aacgcgccgc gcagtatcag cttcagcggg
    18061 ccgtgggcgc agttccgcct gttcggggcc ggacaactga ccggagtaca ggatggcaac
    18121 tttaccgtgc gctttagcgt ggacggtggc gcgatgacct accgtgtgca taccgacacc
    18181 gaggataacc cgttcagcgg tgggttgttc agccagtttg gtctgtcaga cacactgtac
    18241 tgaggaggca gggatgaata gtaacgtact gacacaaact atcgttaccg gcagtgaccc
    18301 gcgcgggctg ccggaattca gcgccattcg cgaggaaata aacaaagcca gccacccgtc
    18361 acagcctgag ctgaactgga aactggtgga gtcgctggcg ctggcgattt ttaaagctaa
    18421 cggtgtggat ttacacaccg ccacctacta tacgcttgca cggacacgga cacagggact
    18481 ggcgggcttt tgcgaaggtg cggaactgct ggcggcaatg gtaagccacg actgggataa
    18541 gttctggcca cagggcggtc cggcgcgtac tgaaatgctg gactggttta actcccgaac
    18601 cggcaatatt ctgcgtcagc aaatctcctt tgcggaatcc gacctaccac tgatctaccg
    18661 cacagagcgg gcattgcagc ttatctgcga caagctccag caggtagagc tgaagcgcgt
    18721 tccgcgcgtg gagaatctac tctattttat gcagaacacg cgtaaacggc ttgaacccca
    18781 gccgaagagt aacgcagaga acgccgcaca gaccacggtc agaacgctga tttatgcccc
    18841 ggaaacaatg gcttcctcca cgccagaagc ggtagtgcct cccctgcccg acctgcctga
    18901 gatgaaagtg gaagtgcgca gtctgtcaga aaatccccca caggccagtg tgatgaagca
    18961 aggcagtacg gtaagagggt ttatcgcagg gatcgtctgt tcagtggcta tcgcctcagc
    19021 attgtggtgg tggcaggtct atccggcgca gcagcaactg ttacaggtta acgacaccgc
    19081 gcagggcgca gcaacggtgt ggatggcctc acctgaactt gaaaactatg agcgcaggct
    19141 gcatcagctt cttggcgtct ccccggtaca accgctggaa accgggatgc agatgatgcg
    19201 tgttgccgac agtcgctggc cggaaagcct gcaacagcag caggcctcga cacaatggaa
    19261 tgaggcactc aaaacccgcg cacagagtag cccacagttg cgcggctggt tgcagacccg
    19321 ccaggactta catgcttttg ctgatctcgt gatgcagcgc gagaaagagg gattaaccct
    19381 ttcctatatc aaaaatgtca tctggcaggc ggagcgggga ctggggcagg aaacacctct
    19441 ggaatccctg ctgacgcagt accaggatgc ccgtgcgcag aagcagaata cagatgcgct
    19501 ggaaaaacag attaacgagc ggatggacgg cgtgttaagt cgctggctgc tgctgaagaa
    19561 taatacgata ccgacgataa aaaaagacat tgaatttcaa taacattcat gaatacaaag
    19621 gagttttaaa tggcgaattt aatttattta acactaaatg gtgataatca agggctcatt
    19681 tcaagtggtt gttcatcaca accatccatt ggaaataaag ctcaaacagc acacattgat
    19741 caaattatga tttatgaatt catgcatgga ttaaatcgag atcaaaacgt caaccatcat
    19801 catttgacaa taaaaaaacc tatagataaa gcatcaccct tattaggcaa agcaatttgc
    19861 gacaatgaat tattgacatg tgacttttcc ttttatcgta caaataaatt tggaattaat
    19921 gaactgtact ataaaatcaa attaactgga gcaaagataa gcgacattca tgtctctata
    19981 tcacatattg ttgttgataa cagcattcaa cccgaagagt cagtttcttt cagttatgag
    20041 tcaattatat gggagcattg ttccgctggc actagtgcat acagtttatg ggaagatcgt
    20101 cttttttaaa aatatctg
//
LOCUS       DAZGAP010000096        19997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGAP010000096 DAZGAP010000000
VERSION     DAZGAP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.096"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..430
                     /locus_tag="ACHLAM_002953"
     CDS             <1..430
                     /locus_tag="ACHLAM_002953"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7854450.1"
                     /translation="SKLWEKYTTVVAMYGATAGEVCIIGDKMAANQACCGLYSKTFPF
                     FVYNFVCNKANELASKATGSAQQNLNKLIISTTKFICPSNDIITIFEDNVTPLFMKWF
                     SNSSENNTLTALRNTLLPKLISGELSVEDLPDHIKQTEAA"
     gene            525..3788
                     /locus_tag="ACHLAM_002954"
     CDS             525..3788
                     /locus_tag="ACHLAM_002954"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO7854451.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEANIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            4003..4341
                     /gene="symE"
                     /locus_tag="ACHLAM_002955"
     CDS             4003..4341
                     /gene="symE"
                     /locus_tag="ACHLAM_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO7854452.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(4383..4436)
                     /locus_tag="ACHLAM_002956"
     CDS             complement(4383..4436)
                     /locus_tag="ACHLAM_002956"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO7854453.1"
                     /translation="MSDERLRDIGLRREDVE"
     gene            complement(4425..4580)
                     /locus_tag="ACHLAM_002957"
                     /pseudo
     CDS             complement(4425..4580)
                     /locus_tag="ACHLAM_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001684533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            4723..6135
                     /gene="yjiR"
                     /locus_tag="ACHLAM_002958"
     CDS             4723..6135
                     /gene="yjiR"
                     /locus_tag="ACHLAM_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7854454.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(6377..7321)
                     /locus_tag="ACHLAM_002959"
     CDS             complement(6377..7321)
                     /locus_tag="ACHLAM_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7854455.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            7788..9020
                     /gene="mdtM"
                     /locus_tag="ACHLAM_002960"
                     /pseudo
     CDS             7788..9020
                     /gene="mdtM"
                     /locus_tag="ACHLAM_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            9061..10341
                     /gene="yjiN"
                     /locus_tag="ACHLAM_002961"
     CDS             9061..10341
                     /gene="yjiN"
                     /locus_tag="ACHLAM_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO7854456.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            10457..11608
                     /gene="yjiM"
                     /locus_tag="ACHLAM_002962"
     CDS             10457..11608
                     /gene="yjiM"
                     /locus_tag="ACHLAM_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO7854457.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            11618..12385
                     /gene="yjiL"
                     /locus_tag="ACHLAM_002963"
     CDS             11618..12385
                     /gene="yjiL"
                     /locus_tag="ACHLAM_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO7854458.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12382..12639
                     /gene="ytiA"
                     /locus_tag="ACHLAM_002964"
     CDS             12382..12639
                     /gene="ytiA"
                     /locus_tag="ACHLAM_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO7854459.1"
                     /translation="MKEHLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12704..13564
                     /gene="yjiK"
                     /locus_tag="ACHLAM_002965"
     CDS             12704..13564
                     /gene="yjiK"
                     /locus_tag="ACHLAM_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO7854460.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13632..14810
                     /gene="yjiJ"
                     /locus_tag="ACHLAM_002966"
     CDS             13632..14810
                     /gene="yjiJ"
                     /locus_tag="ACHLAM_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854461.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14823..15377)
                     /gene="kptA"
                     /locus_tag="ACHLAM_002967"
     CDS             complement(14823..15377)
                     /gene="kptA"
                     /locus_tag="ACHLAM_002967"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO7854462.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDIVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIGFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            15627..16310
                     /gene="yjiH"
                     /locus_tag="ACHLAM_002968"
     CDS             15627..16310
                     /gene="yjiH"
                     /locus_tag="ACHLAM_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO7854463.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            16307..16768
                     /gene="yjiG"
                     /locus_tag="ACHLAM_002969"
     CDS             16307..16768
                     /gene="yjiG"
                     /locus_tag="ACHLAM_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO7854464.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16781..17953
                     /gene="iadA"
                     /locus_tag="ACHLAM_002970"
     CDS             16781..17953
                     /gene="iadA"
                     /locus_tag="ACHLAM_002970"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7854465.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            18018..18929
                     /gene="hypT"
                     /locus_tag="ACHLAM_002971"
                     /gene_synonym="qseD"
     CDS             18018..18929
                     /gene="hypT"
                     /locus_tag="ACHLAM_002971"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO7854466.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPIAERFWRELRELEIEPG"
     gene            18936..19307
                     /locus_tag="ACHLAM_002972"
                     /pseudo
     CDS             18936..19307
                     /locus_tag="ACHLAM_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(19355..19738)
                     /gene="iraD"
                     /locus_tag="ACHLAM_002973"
     CDS             complement(19355..19738)
                     /gene="iraD"
                     /locus_tag="ACHLAM_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO7854467.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGNWHRTMPQ"
BASE COUNT         4823 a         5005 c         5003 g         5166 t
ORIGIN      
        1 atctaaatta tgggaaaaat atacaaccgt tgttgcaatg tacggtgcga ctgcgggtga
       61 agtgtgtata attggtgata aaatggcggc caatcaagca tgttgtggat tgtattcaaa
      121 gacattccca ttttttgtct ataattttgt ctgcaataaa gcaaatgaat tagcatcaaa
      181 agctacaggt tcagcgcaac agaacctcaa taaattaatt atatctacaa caaaatttat
      241 atgcccctca aatgatataa ttactatctt tgaagataat gtaaccccat tatttatgaa
      301 atggttttct aattcgtctg aaaataatac cctaacagcc ctccgcaata ccctgctccc
      361 caaactcatc tccggcgagc tatccgtgga ggatctcccg gatcacatca aacaaaccga
      421 agccgcataa cgcattttgc ccctgtaaaa tcaggggctt tctggtaagg ttttctactg
      481 atacgggaat gcttaccaga aattagccag ggttggagca cgatatgagt ctctctttca
      541 gtgaagcaaa attagaacaa gcgatcattg aactgttaca ggatcaggga tatcaacacc
      601 tgatcggcga taatgtccca cgttcaagtc tcgatcaggt cattatcgaa gacgatctcc
      661 gtcattattt agcggcacgc taccagcctg atggcattac tgaagaagag attcagcgac
      721 tgatcaaaca gttcaccacg cttccggcct ccgatcttta tgaaagcaac aaaacatttt
      781 gtcgctggct ggcaaatggt tttctgttca aacgcgacga tcggcaacaa aaagatctct
      841 atattgaatt gctcgacacc cggcatctac ctgccgcact gcgccagata tttgacgccg
      901 aagatgtccc gttgcaacag gctgcggaac tcccgccttc ctatattaac ccgccgctta
      961 accggattaa gattgttaac cagcttaaaa tctccggcaa agataatcag actcgtattc
     1021 ctgacggcat tctctatatc aacggtctgc cactggtcgt ctttgaattt aaaagcgcgg
     1081 tgcgcgagca ggaagccaat ataggcgatg cctggaagca gctctgtaaa cgctaccgcc
     1141 gggatattcc gcaactgttt atctacaacg cgctctgcat tattagcgat ggcgtaaata
     1201 accggatggg caacctgttt gcaccctatg aatattttta ctcatggcgc aaagtcaccg
     1261 gtaatgaaaa ccgtgaacag gacggaattc catcattgca ctcaatgatt caggggctgt
     1321 ttcatccggt acgtctgctg gatgtgatta aaaactttat ctgcttcccg gataaagcca
     1381 agcacgaagt aaaaatttgc tgccgatatc cgcagtacta tgccgcccgc aaactctatt
     1441 acagcatcaa gcaagcgcgt aaacctttcg gtagcggtaa aggcggtacc tactttggcg
     1501 caacgggctg cggtaaaagc tacaccatgc aatttttaac ccgcttgtta atgaaaagcg
     1561 tggagtttgc cagcccgacc attgttttga tcaccgaccg caccgatctg gacgatcagc
     1621 tttctgcgca aatgtgcaac gccaaaaatt acattggcga cgacaccatc gttcctgtta
     1681 ccagccgtga agatttgcgt aatcaactgg cgggacgcaa tagtggcggt gtattcctga
     1741 caacgatcca taaattcacc gaagacaccg aactcctttc tgaacgcagc aatatcattt
     1801 gcatctcgga cgaagcacat cgcagccagg ttaacctcga ccagaaagtc atcgtcgata
     1861 aagaaagcgg aaaagtgcgc aaaacgtatg gctttgcgaa atacctgcac gattcactgc
     1921 caaatgccac ctatgttggc tttaccggca caccgattga cgcgacgctc gatgtcttcg
     1981 gtgaggtgat cgacagttac accatgaccg aatccgttca ggatgaaatc actgtacgca
     2041 tcgtgtacga aggccgtgcg gctaaagtga tcctggactc cagcaaactg gaagaagtcg
     2101 aaaagtatta cgaagagtgc gcaaacgcag gcaccaatga gtggcaaatc gacgaaagca
     2161 agaaagccac ggctaccatg aatgcggttc tgggtgatga agatcggtta aaagccctcg
     2221 cggaagattt tgctaaacat tatgaaaaac gcgtagccga aggttccacc gtaataggca
     2281 aagccatgtt tgtttgtgcc agccgtgaaa ttgcctggga tttctaccgc cagcttaaag
     2341 ctattcgccc tgcctggttt gaagtgaagc aagcacctga tggcgtcgtc ctgacggaac
     2401 aggaacaaaa agagttaccg ccttctgaaa tggtgaaaat ggtcatgacg cgcggtaaag
     2461 atgacgacga ggcgctttat gatttactgg gcacgaaaga atatcgtaaa gagctggata
     2521 agcagttcaa aaacgctaaa tcgaatttca aaattgccat tgtcgttgat atgtggctga
     2581 ccggttttga tgttcctgaa ctggatacca tctatattga taagccctta caaaaacata
     2641 accttatcca gactatttct cgcgttaacc gtaagctgga aggcaaaagc aaagggttag
     2701 tggtggacta catcggcatt aaaagtcaga tgaaccaggc actggcaatg tattcccgca
     2761 ttgatgccac caactttgaa gatattcagc aatcggtaat agaagttaaa aaccatctcg
     2821 atttgttggg gcaagtcttt tacgaatttg acagtcggga ttattttagt ggtgagccac
     2881 aagcgcaatt atcctgcctc aaccgcgcgg cggaatttgt tctgcgtacc cagaaagttg
     2941 aacgtcgttt tatgggactg gttaaacgca tgaaagccgc ctacgacgtc tgctgcggca
     3001 gcgaagcatt atcacagaca gaacgtgatc atattcacta ttatcttgct gttcgttcaa
     3061 ttgttttcaa actgacgaaa ggtgacgcac cggatgtcac ccagatgaat gcacgcgttc
     3121 gtgaaatgat tgcagaagcg ctaaaagctg atggcgtaga agaaatttat tttcttggcg
     3181 ataaaaaagc ggaatccatc gatatttttg acgaagatta tctggcgcga attaacaaga
     3241 tcaaacttcc ggcaacaaag atccagttat tacaaaaatt actggaaaaa gcgatcagcg
     3301 acttcaagaa agtgaaccag ttgcaaggga ttaacttcac ccgccgcttc caggcgatta
     3361 tagatcgtta taatgagcgg cgagaagatg atgtactcaa cggtgaagaa ttcgatacat
     3421 tcagtcagga aatgaccgat attatctatg atattaaaac cgaaatgggc acctgggccg
     3481 atttaggcat tgatattgaa gaaaaagcct ttttcgacat tcttgctcat atgcgcgata
     3541 aatatcagtt cacctatgac gatgaaaaaa tgctgtcact ggcaaaagag atgaaaagcg
     3601 tggttgacaa cacatcgaaa tatcctgact ggagtaaacg cgatgatatt aaagcgaaac
     3661 tgaaagttga acttattctg cttctacaca agcataagtt cccgccagta gcgaatgatg
     3721 atgtttatat gggtgtgctg gcacaggcag agaattttaa agaacatcat atgagtgctt
     3781 taaattaatt atgaaggccg gatacacttt gttatccggc cattctgaat taacgttaat
     3841 ttctcttcca gatctttcca gtaacgcttt tattattttc tatttttagc tggactttcc
     3901 ctgtatttac tgatgatata tccaggtatt tagcgcggtg cgaatgtgcg ccaacacacc
     3961 cgcaccgctc atcacaatcc ctatttcacg agaatagtaa tcatgactga cacgcattct
     4021 attgcacaac cgttcgaagc agaagtctcc ccggcaaata accgtcaatt aaccgtgagt
     4081 tatgcgagtc gctacccgga ttacagccgt attcccgcca tcaccctgaa aggtcagtgg
     4141 ctggaaaccg ccggttttgc caccggcacg gcggtagatg tcaaagtgat agaaggctgt
     4201 attgtcctca ccgcccaacc acccgccgcc gaagagagcg aactgatgca gtcactgcgc
     4261 caggtgtgca agctgtcggc gcgtaaacaa aggcaggtgc aggattttat tggggtgatt
     4321 agtagcaaaa cgccacgcta agtttctctc cccttcccca tacgggaagg gaaattgccc
     4381 cctcactcca catcctccct gcgtaacccg atatccctca accgttcatc actcatctgc
     4441 tgtacacccg ccgggcctgc atttgccgcc gccaacgcct caccgcctgt cagagttgca
     4501 caagtctgat aaacggcgtt ttggctttgt tttcgtgaaa ttccatctgc tgcctcctca
     4561 tctttcagag gcttcatcat ccgtcttctg gcagttcaca atacagatgc aaatattagt
     4621 tttatttaac atacagacag gttaaaacag catctgcatc cggtttttta gcctcatctg
     4681 taccggtttt tctctctgta tggtccaaat aaggaataca gcatgacgcg ttatcaacat
     4741 ctggcgactc tacttgccga gcggattgag caagggctgt atcgtcacgg ggagaaattg
     4801 ccgtcggtgc gcagcttaag tcaggagcac ggcgtcagta ttagcaccgt gcagcaggcg
     4861 taccagacgc tggagacgat gaagctcatc actccgcagc cgcgttcggg ttattttgtc
     4921 gcacaacgta aagcccagcc gccagtaccg ccgatgacgc gtccggtgca gcgcccggtg
     4981 gaaattaccc agtgggatca agtgctggat atgctggagg cgcatagcga cagttccatt
     5041 gttccgttaa gtaaaagcac gccggatgtc gaaacgccca gcctgaaacc gctgtggcgg
     5101 gagctaagcc gggtggtgca gcataatctg caaaccgttc tcggttatga cttgttagcc
     5161 ggtcagcgag tgttgcgaga gcagattgcc cgcctgatgc tcgacagcgg ctcagtggtc
     5221 accgctgatg acatcattat caccagcggc tgccataact cgatgtcgct ggcgttaatg
     5281 gcggtatgta aaccgggcga tattgtcgca gtcgaatccc cctgttatta cggttcaatg
     5341 cagatgctgc gcggtatggg cgtgaaagtg attgaaatcc caaccgatcc agaaactggc
     5401 atcagcgttg aagcgctgga actggcgctg gaacagtggc cgattaaagg catcattctg
     5461 gtgccaaact gtaataatcc gctgggattt attatgccgg acgcacgcaa acgggccgtt
     5521 ctctctctcg ctcagcgtca tgatattgtg atttttgaag atgatgtcta cggcgaactg
     5581 gcgacggagt atccgcgccc gcggaccatt cattcctggg atatcgacgg gcgagtgctg
     5641 ctgtgcagct cgttcagtaa aagtattgca ccaggcctgc gcgtgggttg ggtcgcaccg
     5701 gggcgttatc acgataaact gctgcatatg aaatatgcca tcagcagctt taatgtaccg
     5761 tccacgcaaa tggcggcggc aacgtttgtt ctggaaggtc actatcatcg ccatatccgg
     5821 cggatgcggc agatctatca gcgcaatttg gcgctttata cctgctggat acgggaatat
     5881 tttccctgcg aaatctgtat tacgcgcccg aaaggcggat ttttactgtg gatagaattg
     5941 cctgaacagg tcgatatggt ttgcgttgcg cggcagctgt gccgcatgaa aatccaggtg
     6001 gcggcaggct cgattttctc agcttccggc aaataccgta attgtctgcg catcaactgc
     6061 gctttgccgc tcagcgaaac ctatcgcgaa gcactgaagc aaattggcga ggccgtgtat
     6121 caggcaatgg aatgaattcg aattagtggc gaggcccctg atttcctctc acgaatcgcc
     6181 tgtgactcgt tttatctttg caggggcatc gccacgctcc agcatggctt aggcaaagtg
     6241 tgctttgttc ataccggatg cggcgtgaac gccttatccg gcttacaaat cgcgcaaatt
     6301 caatagattg cagaggtaat gtaggtctga taagcgtagc acatcaggca gctttgcgtt
     6361 tgtcatcagt ctctgattag tactcgatcc atggatcgcg cttgctttca ggcaagggtt
     6421 gctctaatgc ctgcatttgc tctgccgaaa gtccagtata tcgttgtatc cattcaggat
     6481 ccgcaccgtt ctgcaaaagc aatctaagga ggcgttgttc cccttcttct ttcccctttt
     6541 ccatccctaa cagccatcca tcattatgaa ttcgctctgc aatcgtcatt attcgctccc
     6601 tttgttgtgg cattcgacgg gtaagttcac tgataaactc attaaaacgc gcttcatcgc
     6661 cagtcagtaa aatgtaattt aacagcgcag ttatctggct gtcattagca cactctgtaa
     6721 ccagtaatat taccagttga tcgataagcc ccatcagatc gcgctggcga atatgctttt
     6781 ggatcaactc caacagggcg actctgcgat gctgcacaat ctcgtcgtct ggcacgacag
     6841 tgacatccac cagcgggaac gctgcggtat aaagcttccg tgcggtggtc gggtcagcaa
     6901 attcgtccag ccagcacagg gaccagggat aaggactacg gctaccgtga taaaacagca
     6961 tcgggatgac cagcggtagc ggtcggcgtt tatcatgctc gatatggcgc tgcatcaccg
     7021 ccatggaata tcgcatcagg cgaaaggcca tatggatatc ctcgcggctc tgatgttcaa
     7081 tcactacgta aatataacca tcaccttcac gggtctttac cgaccacaga atatcggagt
     7141 gtagcgcccg caatttttca tcgacgaagc tggcggattc cagttttaag ctgtcgagat
     7201 cgcacagttc acgtaaatct ttgggtaagt ggatctccat aaaatcccgc gcggtgtcag
     7261 ggtgcgtgag aaaggattta aataatgcgt catgcggcgt actggtcgtg aagtttgtca
     7321 tggcgatccg tcacctgttg ataagatggc ccgactttag cgactaacaa aaccattctc
     7381 acccggtaat ttcccattcc ccgaggggct ttccaggcta tttttattct tctgcaaacg
     7441 atacattttt ccggaaacag gctcgcattt tacatttcct gtacccggca gatattcccc
     7501 tttccaccgc actcttttga ttaaccgcaa attttaatta cacttaaggt gtatattttc
     7561 tatgcaaccc atcaattcaa gaggtgtaat gtgctgatga ctatttgtaa tcgttataca
     7621 ttctgacctg gagtcagaag gtatttctct gtctgtgtat tcacaggcag tgtggttgat
     7681 tacatgaaat tcagtacatt tatccttctc gcctgccttt cgtcattacc gacggtattg
     7741 aatttcgttt tccccattgg ggttctccgg acaaggagtt gtttgttatg ccacgttttt
     7801 ttgcccgcca tgccgccacg ctgtttttcc cgatggcgtt gattttgtat gactttgctg
     7861 cgtatctgtc gacggacctg atccagccag ggatcattaa tgtggtccgt gattttaatg
     7921 ccgatgtcag tctggcccct gctgccgtca gtctctatct tgctggcggt atggcgttac
     7981 agtggctgct ggggccgctt tccgacagaa ttggccgcag gccggtgctg attaccgggg
     8041 cgttaatttt tacccttgcc tgcgccgcga caatgttcac cacgtcaatg acacaatttc
     8101 ttatcgcgcg tgcaattcag ggcaccagta tctgttttat tgccaccgtt ggttatgtca
     8161 cggtgcagga ggcgtttgga cagacaaaag ggatcaagtt gatggcgatt atcacctcca
     8221 tcgtactgat tgcgccgatt atcggcccgc tttccggcgc agctctgatg cactttgtgc
     8281 actggaaagt cctttttgcc atcattgcgg ttatgggttt tatctcattt gttggcttac
     8341 tgctggcgat gccagagacg gtgaagcgcg gcgcggttcc atttagcgcc aaaagcgtct
     8401 tgcgcgattt tcgtaatgtc ttttgcaatc ggctgttcct ctttggcgca gcaaccatct
     8461 ctttaagcta tatcccgatg atgagttggg tggctgtctc gccggtgatc ctgattgacg
     8521 caggcggctt aacgacctcg cagttcgcct gaacacaggt tccggtgttc ggcgcggtga
     8581 ttgtcgcgaa tgccatcgtg gcgcgtttcg ttaaagatcc gaccgaaccg cgctttatct
     8641 ggcgcgccgt accccttcaa ctggttggcc tcgcgctgtt gattatcggc aatctgctgt
     8701 caccgcacgt ctggctgtgg tcggtgctgg gcaccagtct gtatgctttc gggattggtt
     8761 tgattttccc gaccttattc cgctttacgc tgttttccaa taacttaccg aaagggaccg
     8821 tctccgcatc gctgaatatg gtgatcctga tggtgatgtc tgtctcggtc gaaatcggcc
     8881 ggtggctgtg gtttaacggc ggtcgcttgc cgtttcatct gttagccgtc gtggcgggcg
     8941 ttatcgtcgt tttcaccctg gcgggattgc tcaatcgcgt gcgccaacat caggctgccg
     9001 agctggcgga ggagcggtga tttttgcgcg atcccgccgt caggctctat tcttaacatt
     9061 atgaataaac tcattgaact cagacgcgcc aaaatgttgg cgctctcttt actgcttatc
     9121 gccgctgcta cctttgtcgt tacgctgttt ttgccgccca atttttgggt gagcggcgtg
     9181 aaggcgattg ctgaagcggc gatggtcggc gcgctggcgg actggtttgc ggtggtggcg
     9241 ctgtttcgcc gcgtgccgat tccgatcatt tctcgtcata cggcgattat cccgcgtaat
     9301 aaagaccgga ttggcgaaaa tctcggtcag ttcgtgcagg aaaaatttct cgatacccag
     9361 tcgctggtgg cattgattcg acgtcacgaa ccggcgttgt tgattggcaa ctggtttagt
     9421 cagccggaga acgcccgccg cgttggtcag catctgttgc agatcatgag cggttttctt
     9481 gaactgaccg atgatgcgcg tattcagcgc ctgcttaagc gcgcggtcca ccgggcgatt
     9541 gataaggttg atctttctgg caccagtgcg ttgatgctgg agagtatgac caaaaacgat
     9601 cgtcatcagg tgctgctcga tacgctgatc gcacagttga tcgcccttct ccagcgcgat
     9661 aaatcgcgca agtttatcgc ccagcaaatt gttcgctggc tggagagcga gcatccactg
     9721 aaagccaaaa ttttgcccac tgaatggctg ggcgaacata gcgcggagtt ggtttctgac
     9781 gcggtgaatt ctttgcttga tgatatcagc cgcgatcgtg cgcatcagat ccgccatgcg
     9841 tttgatcgcg ccacctttgc cctgatcgac aagctgaaaa acgatcctga aatggcagcg
     9901 cgagccgatg ccgtaaaaag ctatctgaaa gaagacgaag cttttaaccg ctatctcagt
     9961 gaattgtggg gggatttacg ggaatggctg aaagtggata tcaacagcga agattctcgt
    10021 gtgaaagaac gtatcgcacg agcgggtcaa tggtttggcg aaacgttaat tgccgatgat
    10081 gccttgcggg cgtcgttaaa tggtcatctc gaacaagccg cgcaccgcgt cgcgcctgag
    10141 ttttccgcat tcctgacgcg ccacatcagc gatacggtaa aaagctggga tgcgcaggat
    10201 atgtcgcggc aaatcgagtt aaatatcggc aaagatctgc agtttatccg tgtcaacggt
    10261 acgctggttg gcggttgtat tggacttatt ttgtatttgc tgtcgcagct cccggccttg
    10321 ttccccctcg gcaatttata gaaatccatg aaaaactaag tggctaaccg cgagagagat
    10381 caaataaacc acactaaaag aatggtaata atgtggcctc ttttcattat ctcccgtggt
    10441 acggggaagg aaaatcatgt cacttgtcac cgatctaccc gccattttcg atcagttctc
    10501 tgaagctcgc cagaaaggct ttctcaccgt catggatctc aaggagcgcg gcattccgct
    10561 ggttggcacc tattgcacct ttatgccgca agagatcccg atggcagccg gtgcggttgt
    10621 ggtttcgcta tgttccacct ctgatgaaac cattgaagaa gccgagaaag atctgccgcg
    10681 caacctctgc ccgctgatta aaagcagcta cggcttcggc aaaaccgata aatgccccta
    10741 cttctacttt tctgatctgg tggtcggtga aaccacctgc gacggcaaaa agaaaatgta
    10801 tgaatacatg gcggagttta agcccgttca tgtgatgcaa ctgccaaaca gcgttaagga
    10861 cgatgcctcg cgtgcattat ggaaagccga gatgctgcgc ttgcaaaaaa cggtagaaga
    10921 acgttttggg cacgagatta gcgaagatgc tctgcgcgat gccattgcgc tgaaaaaccg
    10981 cgaacgtcgc gcactggcca acttttatca tttggggcag ttaaatcctc cggcgcttag
    11041 cggcagcgac attctgaaag tagtctacgg cgcaaccttc cggttcgata aagaggcgtt
    11101 gatcaatgaa ctggatgcaa tgaccgcccg cgttcgtcag cagtgggaag aaggccagcg
    11161 actggacccg cgcccacgta ttttaatcac cggctgccca attggcggcg cagcagagaa
    11221 agtggtgcgc gcgattgaag agaatggcgg ctgggttgtc ggttatgaaa actgcaccgg
    11281 ggcgaaagcc accgagcaat gcgtggcgga aacgggtgat gtgtacgacg cgctggcgga
    11341 taaatatctg gcgattggct gctcctgtgt ttcgccgaac gatcagcgcc tgcaaatgct
    11401 cagccagatg gttgaagaat atcaggtcga tggcgtagtt gatgtgattt tgcaggcgtg
    11461 ccatacctac gcggtggaat cgctggcgat taaacgtcat gtgcgccagc agcacaacat
    11521 tccttatatc gctattgaaa cagactactc cacctcggat gtcgggcagc tcagcacccg
    11581 tgtcgcggcc tttattgaga tgctgtaagg agtggcagtg acatattcga ttggcattga
    11641 ttccggctca accgccacca aagggatctt actggctgac ggcgtgatta cgcgccgttt
    11701 cctcgttcca acatcctttc gcccggcaac agcaattact gaagcctggg aaactctgcg
    11761 cgaagggtta gagacaacgc cgtttctgac gctcaccggc tacggacggc aactggtgga
    11821 ttttgccgat aaacaggtga cggaaatctc ctgtcacggg ctgggcgcac ggtttcttgc
    11881 gccagcaacg cgcgcagtaa tcgacatcgg tggtcaggac agcaaagtga ttcagcttga
    11941 tgatgacggt aacctgtgcg atttcctgat gaatgacaaa tgcgcggcgg gcaccgggcg
    12001 tttcctggag gtgatctcgc gcacgcttgg caccagcgtc gaacagctcg acagcattac
    12061 cgaaaatgtc acgccgcacg ccatcacgag tatgtgcaca gtgtttgctg aatcagaagt
    12121 gatcagcctg cgctctgcgg gcgtagcgcc agaagcgatt ctcgccggag tgattaacgc
    12181 gatggcgcgg cggagtgcca atttcattgc tcgtctctcc tgtgaagcgc cgattctgtt
    12241 tactggtggc gttagccact gtcaaaggtt tacccacatg ctggaaagcc atctgggaat
    12301 gccggtacaa actcaccctg atgcgcaatt cgctggtgca attggcgcgg cggtgatcgg
    12361 tcagcggcag agaaaacgcg catgaaagag catctgtttt tatttcactc cacggtcggc
    12421 gtcatacaaa cccgcaaggc gttacaggca gcgggcatga cttttcgcgt cagcgatatt
    12481 ccccgtgatt tacgcggcgg ctgcgggtta tgcatttggc tgacctgtcc gcctggcgag
    12541 gaaattcaat gggtgatccc tgggcatacg gaatcagttt attgtcagca ggatggtggc
    12601 tggcgctgca ttgcacatta cgggatttct ccgcgttaag gattcattaa gatcgctacg
    12661 tatacttctc gatatcttat tgatttgcct taaagggatt accgtgacaa aaagtatttc
    12721 attaagtaaa cgcatcttcg ttattgtgat tctttttgtc attgtggcag tgtgtacttt
    12781 cttcgtgcaa tcttgcgccc gtaaaagcaa tcatgcagca tcatttcaga attaccacgc
    12841 caccattgac ggcaaagaaa ttgcaggcat aacgaataat atctcttcgc tgacctggtc
    12901 ggcacaaagc aatactcttt ttagcactat caacaaacct gccgccattg ttgaaatgac
    12961 caccaatggt gatttgatcc gcacaattcc tctggatttt gttaaggatc tcgagacgat
    13021 cgaatacatt ggcgataatc agtttgtcat tagcgatgag cgcgattacg ccatttatgt
    13081 gatttcactg accccaaact cggaagtaaa aatcctcaaa aaaataaaaa tcccgctgca
    13141 ggaatcccca accaattgcg gttttgaagg attggcctat tctcgccagg atcacacttt
    13201 ctggtttttt aaagagaaaa acccgattga ggtctacaaa gttaacgggc tattaagtag
    13261 caatgaattg cacatcagca aagacaaggc actacaacgg caatttactt tagatgatgt
    13321 gtccggcgca gaatttaacc aacaaaaaaa tacgcttctg gtgctgtctc atgaatctcg
    13381 cgcactccag gaagtaacgc tggttggcga ggttatcggt gaaatgtcac taaccaaagg
    13441 cagtcgaggt ttgtcgcata acatcaaaca ggccgaaggg gttgcgatgg atgcctctgg
    13501 taatatttat atcgttagcg aaccaaatcg tttttatcgc tttacccctc aatcatcgca
    13561 ttaacaggct ggcatgcttc acctttttgt tattaacttg ttaacaacaa aaatgacaaa
    13621 caaggttaaa catgccttcg tccacgcatc ccgtagaacg cttttctttc agcaccgcgc
    13681 ttttcgggat gctggttctg accttaggta tgggtttagg ccgcttcctt tatacgccta
    13741 tgttgcccgt catgatggcg gaaggatcgt tttcatttag ccagctctcg tggattgcca
    13801 gcggcaacta tgcggggtat ctggctggca gtctgctatt ttcttttggc gcatttcacc
    13861 agccatcgcg cctgcgccca tttctgttgg cttctgccct ggcgagcggg ttgttgatcc
    13921 tcgcaatggc atggttgccg ccatttattc tggtgttatt gattcgcgtc ctggcgggtg
    13981 tcgccagcgc cggaatgctg atttttggtt cgacgctgat tatgcagcac acgcgccatc
    14041 cttttgtgct ggcagctttg ttttctggcg ttggcattgg catcgcactg ggcaatgaat
    14101 atgttctggc aggcctgcat tttgacctct cctcgcaaac gttatggcaa ggcgccgggg
    14161 cgctttctgg catgatactg attgcactta cgcttttaat gccctcgaaa aaacacgcca
    14221 tcgtaccaat gccattggca aaaacggagc aacagataat gagctggtgg ttactggcta
    14281 ttctgtacgg cctggcggga tttggttata tcatcgtcgc gacttacctg ccgctcatgg
    14341 cgaaagatgc aggctcacca ttgttaaccg cccatctctg gacgttagtc ggcttatcaa
    14401 ttgtgcctgg ttgctttggc tggctatggg ccgcaaaacg ttggggggct ttgccctgcc
    14461 tgacggcgaa tttgctggtg caggctatct gtgtgctgct tactctcgcc agcgactcgc
    14521 ctctcttgct tatcatcagc agtcttggtt ttggcggtac ctttatgggc acgacatctc
    14581 tggtgatgac tatcgcccgc caactgagcg taccgggaaa tctcaatctt ttaggctttg
    14641 tgacgctcat ttacggcatt gggcaaatcc tcggcccggc gctgaccagt atgctcggca
    14701 atggaacatc ggcgcttgcc agcgccactc tctgcggcgc ggcggcgttg tttattgctg
    14761 cgcttatcag cacagtgcaa ttatttaagc tacaagtggt cacttcgtga gagaagtttc
    14821 ccttaccact caagaaactc aaccgcaact gttgatgtca gccagacccc gttttccgct
    14881 tgccaaaaag ggatgcctcg tttcgccatt tcctgtgctt tgacggttaa aataactggc
    14941 gatccgtgtc ttgctcccac tttgcgtgcc gttgcttcat cagcagagag atgcacgtaa
    15001 tggcgctccc ctgcaatcag cccttgtttt tttatctcgt caagaaaacg gcttgccgta
    15061 ccatggtaga gaaattgtgg cggtgttttt tcgatgaagc caatggcgac ctgcgaagtc
    15121 gaatgcccct gaacggcgcg aatacacgta ccatcactgg aataactaaa gcgcttttta
    15181 tcgctggtag cgacaacgat atccagtaat gcgcgggtta accgcttgcc cgctttttgg
    15241 gcacagagta tgagcttatc gatatccgcc catccttcac ggtccagcac gatgccaatc
    15301 gcctctggtt tatgtcggag gacaaaactt aaaaacttgc tcgtctctgc cagctctttg
    15361 tcgttgtatt ttgccatctc gttctcgtcc ctggattttc tgtttttatt tactgatgtc
    15421 attgtcactg ggtttatgaa ttttctgcaa ctcaatcctg cgcagaacct ttgccacaca
    15481 gcgtcatttt tccgctatgc aaaaaacgca tagccagaaa catacttgca ttttccccat
    15541 acccacatgg ctgataaggt ttttcagctc caggaatttt cctggctcaa gcaacacaca
    15601 aacacaaaaa aacaatgggg attgttatga cacaacaagg ggatgctgta gccggggagc
    15661 tggcgacaga aaaagtgggc attaaagggt atctggcttt ttttctcacc ataatctttt
    15721 tttccggtgt tttttccggg acagacagtt ggtggcgcgt ttttgatttt tccgtgctga
    15781 acggatcgtt tgggcaactg cctggtgcga atggcgccac cacctcgttt cgcggcgcag
    15841 gtggcacagg agctaaagat ggctttctct tcgcgctgga actggcaccg tcagtcattc
    15901 tctctctggg gattatttca attaccgatg gtcttggcgg attacgcgcc gcgcagcagt
    15961 taatgacgcc cgtgctaaaa ccgttgttgg gcattccagg catctgttca ctggcgctga
    16021 ttgctaacct gcaaaacacc gatgccgccg caggtatgac caaagaactg gcgcaggaag
    16081 gggaaattac cgaacgtgat aaagtgatat tcgccgccta tcaaaccagc ggtagcgcca
    16141 tcatcaccaa ctacttctct tccggcgtcg cggtgtttgc ctttctgggc acgtccgtaa
    16201 tcgtaccttt agccgtcatc ctggtgttta aatttgtcgg agccaacatt ttgcgcgtct
    16261 ggcttaactt cgaagaacgt cgcaacccaa cgcaaggagc gcaagcatga caactcaggt
    16321 ccgcaaaaac gtcatggata tgtttattga tggcgcgcgc cgtggcttta ctatcgctac
    16381 caccaacctg ctgccaaacg tggtgatggc ctttgtcatc atccaggcgc tgaaaattac
    16441 tggcctgctc gactgggtgg gacatatctg tgaacccgta atggcgctgt ggggattgcc
    16501 cggtgaagcg gcaaccgtcc ttttagccgc attgatgagc atgggcggcg cagtcggtgt
    16561 tgccgccagt ctggcaaccg ctggcgcatt aaccggccac gatgtcaccg ttttactgcc
    16621 cgcgatgtat ttaatgggca acccggtgca aaatgtcggt cgctgcctgg gaacagccga
    16681 agtgaatgcc aaatattacc cgcatatcat tacggtctgc gtgattaacg cattgctgtc
    16741 gatctgggtt atgcagctta ttgtttaaca aggagttacc atgattgatt attccgcagc
    16801 cggttttacc ctgctgcagg gagcgcattt gtatgcgccg gaagatcggg gaatttgcga
    16861 tgtcctcatc gctaacggca aaattattgc cgttgccagc aacatccctt ctgacattgt
    16921 accagactgc acggttgtcg atctcagcgg gcagatcctc tgcccaggtt ttattgatca
    16981 acacgtccat ttgattggtg gtggcggcga agcaggtccc acgacacgca cgccggaagt
    17041 ggcgttaagt cgtctgacgg aagcgggcgt cacgtcagtg gttggtctgc tgggtaccga
    17101 ctctatctct cgccacccgg aatccctgct cgccaagacc cgtgcgctca atgaagaagg
    17161 catcagcgcc tggatgctga ccggcgctta tcatgtccct tcccgcacca ttacaggttc
    17221 cgtggaaaaa gacgtggcga ttatcgatcg cgtgattggt gtgaaatgcg ccatctctga
    17281 tcaccgttct gccgcacctg acgtttatca cctggctaat atggcggcgg aatcccgcgt
    17341 tggcggtttg cttggcggta aacctggcgt caccgtgttc cacatgggcg acagcaaaaa
    17401 ggcgttacag cctgtctatg acctgctgga aaactgcgat gtgccgatca gcaagctgct
    17461 gccgacccac gttaaccgca acgttccgtt gtttgagcag gcgctggagt tcgcgcgcaa
    17521 aggcggcacc atcgacatca ccagtagcat tgacgaaccg gtcgcccctg ccgaaggtat
    17581 tgcccgcgcc gttcaggcgg gtattccgct ggcacgcgtc accctcagct ccgacggcaa
    17641 cggtagccag ccgttctttg atgacgaagg aaatttaacc catatcggtg ttgcaggttt
    17701 tgaaacattg ctggaaaccg tgcaaatgtt ggtcaaagac tatagcttca gccttagcga
    17761 tgccctgcgc ccgctcacca gtagcgtagc cggtttcctt aacctgaccg ggaaaggcga
    17821 aattctgcca ggcaatgacg cagacttact ggtcatgacg cctgaattgc gcattgagca
    17881 ggtatacgct cgcggcaaac tgatggtcaa agacggcaaa gcctgcgtga aaggaacgtt
    17941 tgaaacggca taaccggttg tcagccgccc gaatggacgt agaatgcccg caagggcggc
    18001 tgacagagta taacgtaatg gatgactgtg gtgcggtttt gcataacatt gaaaccaaat
    18061 ggctttatga ttttctgacc ctggaaaaat gtcgcaattt ttcccaggcg gcagtcagtc
    18121 gcaacgtctc gcaaccggca ttcagccgcc gcatccgtgc actggaacag gcgattggcg
    18181 ttgaattgtt taaccgccag gtgacgccgc tgcaactctc ggaacaaggg aaaatcttcc
    18241 attcgcagat ccgccatctg ttgcaacagt tagaaagcaa cctggcagag ctgcgtggcg
    18301 gcagcgatta cgcgcaacgt aaaatcaaga tagccgctgc acactctctt tccctcggtc
    18361 tgttaccgtc cattatcagc cagatgccgc cgctctttac ctgggcaatt gaagccattg
    18421 atgtcgatga agcggtcgat aaactgcgcg aagggcaaag tgactgtatt ttttcctttc
    18481 acgacgaaga tttgctggaa gcgccgtttg accacattcg cttatttgaa tctcaattat
    18541 tccccgtctg cgccagtgac gaacacggag aagcactgtt taacctcgcg cagccacact
    18601 ttccgttact gaattacagc cgcaactcct acatggggcg attgattaat cgcaccctga
    18661 cgcgccacag tgagttgagt ttcagcacct tttttgtctc ttcgatgagc gagctattaa
    18721 agcaggttgc cctcgacggc tgtgggattg cctggctgcc ggaatacgct atccaacaag
    18781 aaattcgcag cgggcagctc gttgtactga atcgggaaga actggtgatc ccgattcagg
    18841 cctacgcata ccggatgaat acccgcatga atccaattgc cgaacgcttc tggcgtgaac
    18901 tgcgcgagct ggagattgaa cctggttgaa agttttctgc agaaaatctt ccggcgctgg
    18961 gaattatgat tgaggttcct gccgcagtca tgattgcaga caaattagcc agtgaagtcg
    19021 atttcttttc catcggcacc aacgatatga cgcaatatat tatggcggca gacagaggaa
    19081 atagcaccgt cgcgaaactg gtcgattatt gtaatgacgc ggtgattaac gctattgcta
    19141 tggtgtgtca ggccggacgt aataatgaaa ttccggtcag tatgtgcggc gaaatggctg
    19201 gagatatcca gcaaacagca cgactgctca ccatggggat tgataagctc agtgccagtc
    19261 catcccggct tccggccttg aaagcagcaa tcagagcctc ccactaattg tgacctaacg
    19321 cgatacgtct tgcgcgtatc gcgtttcccg tcatttactg cggcatcgtg cgatgccagt
    19381 tccccttctg ccagaccagg tcaaacgtta gcggctcatc atcgtggtac agcatcatct
    19441 ccagactaat ggccaactcg tggcaatagg cttctttcag attgacctgc aatgccgcaa
    19501 tacgtggctc atgaaataaa agcgcggaac gtaactggcg gcaataccaa ctctggagaa
    19561 gcttgtcgct ctgattatgc ccttgccact caggcatgcc tgaaacaagt aatattggta
    19621 gcggtgaatg gggagaacta aaaagttgaa agagatcccg gcatacggaa tcacgcagcg
    19681 ataacgtatt gtgtcctgta ctttcaggta ataccgtctg aactgattgg cgcatcattt
    19741 tgcgcactcc tgacgtttag tggcatctgt atcctgccct ggctgccgga caccactggg
    19801 ttgatgctca tattccataa tcattccttt atgctcattg cgtccctggc actttcataa
    19861 gggatttata agatccgcca aagataaaac actgtacaga aaagaatatc cactgacttt
    19921 ttatttaaaa aggtatcatt tgacaaaagt cattaaacat aacggaaata cgttaacata
    19981 ttctgattat tccgaat
//
LOCUS       DAZGAP010000097        19940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGAP010000097 DAZGAP010000000
VERSION     DAZGAP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.097"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACHLAM_002974"
     CDS             complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACHLAM_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO7854468.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1572..2765)
                     /gene="hemX"
                     /locus_tag="ACHLAM_002975"
     CDS             complement(1572..2765)
                     /gene="hemX"
                     /locus_tag="ACHLAM_002975"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO7854469.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQADAPAA
                     APQGE"
     gene            complement(2787..3527)
                     /gene="hemD"
                     /locus_tag="ACHLAM_002976"
     CDS             complement(2787..3527)
                     /gene="hemD"
                     /locus_tag="ACHLAM_002976"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO7854470.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CVIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3524..4465)
                     /gene="hemC"
                     /locus_tag="ACHLAM_002977"
     CDS             complement(3524..4465)
                     /gene="hemC"
                     /locus_tag="ACHLAM_002977"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO7854471.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4852..7398
                     /gene="cyaA"
                     /locus_tag="ACHLAM_002978"
     CDS             4852..7398
                     /gene="cyaA"
                     /locus_tag="ACHLAM_002978"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO7854472.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     CNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7438..7758)
                     /gene="cyaY"
                     /locus_tag="ACHLAM_002979"
     CDS             complement(7438..7758)
                     /gene="cyaY"
                     /locus_tag="ACHLAM_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO7854473.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7806..9863)
                     /locus_tag="ACHLAM_002980"
     CDS             complement(7806..9863)
                     /locus_tag="ACHLAM_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854474.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFLVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10205..10759)
                     /locus_tag="ACHLAM_002981"
     CDS             complement(10205..10759)
                     /locus_tag="ACHLAM_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO7854475.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11101..11304
                     /gene="yifL"
                     /locus_tag="ACHLAM_002982"
     CDS             11101..11304
                     /gene="yifL"
                     /locus_tag="ACHLAM_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7854476.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11341..12165
                     /gene="dapF"
                     /locus_tag="ACHLAM_002983"
     CDS             11341..12165
                     /gene="dapF"
                     /locus_tag="ACHLAM_002983"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO7854477.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12162..12869
                     /gene="yigA"
                     /locus_tag="ACHLAM_002984"
     CDS             12162..12869
                     /gene="yigA"
                     /locus_tag="ACHLAM_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO7854478.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12866..13762
                     /gene="xerC"
                     /locus_tag="ACHLAM_002985"
     CDS             12866..13762
                     /gene="xerC"
                     /locus_tag="ACHLAM_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO7854479.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13762..14478
                     /gene="yigB"
                     /locus_tag="ACHLAM_002986"
     CDS             13762..14478
                     /gene="yigB"
                     /locus_tag="ACHLAM_002986"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO7854480.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14562..16724
                     /gene="uvrD"
                     /locus_tag="ACHLAM_002987"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14562..16724
                     /gene="uvrD"
                     /locus_tag="ACHLAM_002987"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO7854481.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16769..17671)
                     /locus_tag="ACHLAM_002988"
     CDS             complement(16769..17671)
                     /locus_tag="ACHLAM_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO7854482.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17754..18518)
                     /gene="yigE"
                     /locus_tag="ACHLAM_002989"
     CDS             complement(17754..18518)
                     /gene="yigE"
                     /locus_tag="ACHLAM_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO7854483.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18680..18736
                     /gene="ysgD"
                     /locus_tag="ACHLAM_002990"
     CDS             18680..18736
                     /gene="ysgD"
                     /locus_tag="ACHLAM_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO7854484.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18888..19838
                     /gene="corA"
                     /locus_tag="ACHLAM_002991"
     CDS             18888..19838
                     /gene="corA"
                     /locus_tag="ACHLAM_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO7854485.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         4629 a         5196 c         5208 g         4907 t
ORIGIN      
        1 cgcaggagaa atattcactc aggaagttat tactcaggaa gcaaagagga ttacagaatt
       61 atctcataac aagtgttaag ggatgttatt tcccggttct ctgtggcata ataaacgagt
      121 agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg aatgacgcag
      181 agccgtttac ggtgcttatc gtccactgac agatgtcgct tctgcctcat cagacaccat
      241 ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt aacgcctgct
      301 ccgtaataag agcaggcgtt tttttatgta tcagaaaggt cccggaggag cttgcctccg
      361 ggtgagaaag agctactgtg gcgggttgtt ctgcaacgtt aacatcaaac cgtcgcgacg
      421 catcgctgcg gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa gccatgcgta
      481 atcgtaggcg tccggacgtt gtttcagcgc tgcgcggaag gcgagcgatg cttcctgcca
      541 ttcgccgtgc ttcatcagcg actgacccag tgtgctccac aacagcgggc gatcgcccac
      601 gtttttgatc tgttggcgca gcactttttc aagctgttcc gggttattgg ttttcagacg
      661 cgggatcggc agcagcaggc gatcgtcata ctggcgtttc aggccgtcga tgataatttg
      721 ctgggcagta tcatgatcgt cacattcaat aagatgttcc gccattgcca cctgtaacgc
      781 cacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac cttcactacc
      841 gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca gcattgcacg
      901 atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca gcagcgaact
      961 ccatgcacca gtgcggatat acgcctgttc cgccagacgt aatacttccg gatggcgtgg
     1021 cgtaacttcc agcagcttat caacgccgtg acgtgctgca tggttttcat tacgggccag
     1081 ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca gctccgctgc
     1141 gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg cttcggctgc
     1201 cagtagatag ttcaccaccg gttgttcagc gtgatcggca tttttcgcca tcagcttttc
     1261 aacttgctga taatcgcctt ccgccagttt cagcagcgcc tgttcggtct gcttacgtgc
     1321 acggcgacgc ttacgtccga caaaccaccc acgggtgtgc gcgcctgtgc ggaagattcg
     1381 ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaaaa taatcgcgag
     1441 gcccgtgacg ctagtttcaa tattgtagtt gtcggtctgg atcagcacgt aaccctgatg
     1501 gccggcaatc atcgggccaa ccacgatccc cgcaatcagc aacacaaaga gcaataacac
     1561 ttttagcatg gttattctcc ttgcggcgcg gctgccggag catcagcttg cggtgcaggt
     1621 gcaggtgcag gtgcaggttt agcttccgtt gtccccgctg ccggttgtgc cagcaggtta
     1681 cgcacgcgag tttgcatcag tttttccagc atcgcctggc tttgcagggt ttccggaaga
     1741 tccatcgaga tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg
     1801 gcatcatcag tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga
     1861 taagtctctt cctggtgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata
     1921 ttttcgcgca gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga
     1981 cggcgaatcg taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc
     2041 cattcgctga tggaactgga aagctcttca ccgtcggaat ccatcggcga accatcgctg
     2101 tcattatcag ccagacgcag attatctacc tgatttgaaa gctgattaag cttaaggatg
     2161 ataccgtcat aatccacctg cgataccgca gaaaggctgg cgatatcatc ggtaattgcc
     2221 cgacgaacgg taatcagact cgggtcattc atatccgcca ggctggcatc tgcacttttc
     2281 agcaacgctg cagcggtcgt gacgtcttga tcgctccaca gcttccgtcc tgcgagtttc
     2341 accaggaaat cagcctgagc cagcagccag gttttagcat cgctgccgga aatagtcgcg
     2401 accttttgtt ggacttcatc cagctgtttt gccagcgttt cttgctgacg attcgcctgc
     2461 tcaagctgta cagcttgttg cttgataatg ccttccagct cagctttttg gctctcctgg
     2521 gctttttgca gtgccgtaag ttggttagcc agggcatcgc tggtggcagt ctgattgacg
     2581 gcctgttgtt taccccagcc atacaaaccg acgcccgccg ccagagcaat agcgatagcc
     2641 accgcgctga gaatcaatgc ggtattgttc ttactctttt tttctgttgc gacaggttgt
     2701 gacgtggtgt ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc
     2761 gtcattatgg cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca
     2821 gcgttatcgg cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc
     2881 tcactgacga ccaatagtcg acagcgtagt aaccagtgct cacgatacca ttgtgggatc
     2941 agcgaccaga gttgctgcaa catttcaccg ctggtaacaa cgaccgtcgt cacctcgcgg
     3001 gattgccagc gcatcgcttc ttctgcacca tcgtaatgga ttacgcatcg ttgataacat
     3061 tcacaaaaag tgacctcagc accgcgcgcc gtcagggtat ccccaattag ctcacgaccg
     3121 ccattgccac gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt
     3181 agcaagactt cgctgatttc ccgatcctgc gggtagagaa tcttctgtcc gcttacggta
     3241 tgtagtgcca gtgcggtggt gcgtccaatg gcgaaataag taggtagtcg gggccattta
     3301 cgatcttgct gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac
     3361 aacagatcgc tctcccccag cgccgccagt tgatcagcaa gttgtggtaa ttgtcgaccc
     3421 ggagaaaact caatcagtgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc
     3481 actaactctt ctccagcggg agacgggcgg gtgacaagga tactcatgcc ggggcgtctc
     3541 cattatagac ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg
     3601 aaatccccat ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatctgcg
     3661 aaccgtccgg cgcgccgacc agcgcacgca gccagatttc accatcaata agctcggcgt
     3721 agctaccaat tggcacctga catccgcctt cgagacgggt attcatggcg cgttctgcgg
     3781 taacgcgcag tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat
     3841 catcaaggcg gcattcaata ccgaccgcac cttgtcctac cgccggaaga gaaatctcgg
     3901 gtggtaacgc ggcgcgaata cgtgactcca gacctaaacg ttttagtccg gctacggcaa
     3961 gaatgatggc atcgtattcg ccgttatcca gtttactcag gcgagtgccg acgttgccgc
     4021 gcagggagcg gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac
     4081 tggacgtccc gacgatactg cctgccggta acgcatccag actttcatag ttattggaca
     4141 caaaggcatc gcgaggatct tcacgctcgc aaatggtgac cagccccaga ccttgcggga
     4201 attcaaccgg cacatctttc attgaatgta cggcgatatc ggcgcgattc tccaggagcg
     4261 cgacttccag ctctttaaca aataagcctt ttccgcctac tttcgccagc ggcgtatcaa
     4321 gaatcacatc gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg
     4381 ccatcaactt gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg
     4441 caattcttaa aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct
     4501 aacatccttg ccagagcgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt
     4561 aaaatgaggt ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta
     4621 cagagaataa acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa
     4681 ggtgttaaat tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg
     4741 cgaaaagtgg taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt
     4801 tgatgttggc ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc
     4861 tatattgaga ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg
     4921 cttgctgcta tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac
     4981 tatcaccatc cgctaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt
     5041 tacacgcctg atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg
     5101 tcagtacagg atccgccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc
     5161 acctcgtccg tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc
     5221 tggctcgata gcgaagagcg ccagttacta caacgtaaat gcagcctgct ggaaagctgg
     5281 gccgcctcgc tgggcgtgga agttagcttc ttcctgattg atgaaaatcg cttccgtcat
     5341 aatgaaagcg gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt
     5401 gacgaatttt accgtaccgc cgtgcgtctc gctggtaagc gtattctgtg gaatatggtg
     5461 ccgtgcgacg aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg
     5521 ctgacgccaa atgaatggct ggatctcggt ggcttaagct cgctttctgc tgaagagtac
     5581 tttggtgcca gcctttggca gctctacaag agtatcgatt ccccatacaa agcggtgctg
     5641 aaaacactgc tgctggaagc ctattcctgg gaatacccga acccacgtct gctggcgaaa
     5701 gatatcaaac agcgtttgca cgacggcgag attgtgtcgt ttggcctcga tccttactgc
     5761 atgatgctgg agcgtgttac tgaatacctg acggcgattg aagatttcac tcgtctggat
     5821 ttagtacgcc gctgcttcta tttaaaagtg tgcgaaaaac tcagccgtga acgcgcctgc
     5881 gtaggctggc gtcgtgaagt gctaacccag ttagtgaaag agtgggaatg ggacgatgct
     5941 cgtctggcaa tgctcgataa ccgcgctaac tggaagattg atcaggtacg tgaggcgcac
     6001 aacgagttgc tcgacgcgat gatgcagagc taccgtaatc tgattcgctt tgcgcgccgc
     6061 aataacctta gcgtctccgc cagcccacag gatattggcg tactgacgcg taaactgtat
     6121 gccgcgtttg aagcgttacc gggtaaagtg acgctggtaa acccgcagat ttcgcccgat
     6181 ctctcggaac cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt
     6241 tggtatctgt ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa
     6301 tataaccgtt acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg
     6361 cgtacccgct tgtatattaa aggcaacggc attgtcgatt tgcctaagtt gcaggagatg
     6421 gtcgccgatg tgtcacacca tttcccgctg cgcctgcctg caccgacgcc aaaagcgctc
     6481 tacagcccgt gtgaaattcg ccatctggcg attatcgtta acctggaata tgacccgaca
     6541 gctgcgttct gcaatcaggt ggtgcatttt gatttccgta agctggacgt cttcagcttt
     6601 ggcgagaatc aaaattgcct ggtaggtagc gtggacctgc tgtaccgcaa ctcgtggaac
     6661 gaagtgcgta cgctgcactt caacggcgag cagtcgatga tcgaagccct gaaaaccatt
     6721 ctcggtaaaa tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgctacagc
     6781 cagcatctgc gcggattaat ccgtactcgc gtgcagcaac tggtttctga gtgcattgaa
     6841 ctgcgtcttt ccagcacccg ccaggaaacc gggcgcttca aggcgctgcg cgtttctggt
     6901 caaacctggg gcttattctt cgaacgcctg aatgtatcgg tacagaaact agaaaatgcc
     6961 atcgagtttt atggcgctat ttcgcataac aaactgcacg gcctgtcagt gcaggttgaa
     7021 accaatcacg tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag
     7081 ttcttcttcg aagaaacgca agacgagaat ggctttaata tctacattct cgacgaaagc
     7141 aaccgggttg aggtgtatca ccactgcgaa ggcagcaaag aggagctggt gcgtgacgtc
     7201 agtcgcttct actcgtcatc gcacgaccgt tttacctacg gctcaagctt catcaacttc
     7261 aacctgccgc agttctatca gattgtgaag gttgatggtc gtgaacaggt gattccgttc
     7321 cgcaccaaat cgattggcaa catgccgcct gccaatcagg atcacgacac gccgctatta
     7381 cagcagtatt tttcgtgatg agcgtgccgg aaagcgaggc ttatccggca tgcaatctta
     7441 gcggaaactg actgtttcac ccgcttgctg cgttgccgcc tgttccagca aatcccagaa
     7501 ggtttcgccg ctacgatcgc aaatccactc atcgcctttc agatcaaaat ggtagccgcc
     7561 ctgtttggtt gccagccata cctggtgcag cggttcctgg cggttgataa tgattttgct
     7621 cccgttttca aaggtaatgg tcagtacacc gccgttgatt tcgcagtcga tatcgctatc
     7681 gccatcccag tcgtccagac gctcttcaat ggtcagccac agttgatcag ccaggcgatg
     7741 aaattcactg tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca
     7801 tggggtcaga gcttcaaagt ttgctcaatg tctggtgttt tgtggcactc ttcctccgtc
     7861 gtcaagatga tgatgtcatc attggatgaa aacatgttct gatccagatt acgtgtcctg
     7921 gccggacagg gcgatgcggt tcgataaagt tggctaccga tccaggtata aacatagcga
     7981 ttgtcataag cgaaacgttc ggtggttttc aatggtccat caatagcaaa aatttgaata
     8041 tcatcgcgta aacgggaaga gcgcataaag taaccatggc ctttcccggc gttaatccgt
     8101 tgtttcagga aaggtgcaga agagtcgagc gttattattt ccagggtgtc ttcaccaaaa
     8161 ggagacaatt tcgcaatgta cagatgattt ttgtactgcg cgacacgatc ggtgatggga
     8221 tgaaatgctt tcgggtcaat atcggggagt ttctcccatt gactccattg ctggtctgta
     8281 gagcgaagct tacgccagct aacatcggtt tcgccgacga gaaaagagcc ttcgccatca
     8341 gaatattgag caatgttaac ggatgagtta taggggattc ccgttggaat aaacgcacca
     8401 tcctcaagaa gtctgccgtc aagctcacca ctacggtagt ttgcccaata gccatatcct
     8461 gaatctttgg ttttccattt cacccgcacg gctttggtat tgggagttgg gtagcgccaa
     8521 agctgtgagc catcgaatgc gtaaacataa cgatcgtcgt gtgcatacca tttcttgtaa
     8581 tgggggctat cgctaaggtg agcctcatgc tcgtcaggaa cacgataatc catcaggata
     8641 agcggcccgg aagaatcgaa tacctggagg ccatcttctt cttcaccatc agctggtaat
     8701 ttggtcaata aatagccgtg agttcttccc gcgttaaagc gtttatctat caccaaacca
     8761 ggattatcca gtgtgacgat atttagccta ttttcgccaa atcccgtttt tttgatggtg
     8821 tacagcttgt cctgatactg agcaacggta ttactaagag gatgaaattg ttctggatcg
     8881 aggccgggta ggacttcttg ctggcattct ggccctttgc gccatgaaac agagtgttcc
     8941 tgcaaagtga acgccgcgca gctatttgcc ctatctttgt ccagatttcc ctgattcagc
     9001 accttccggt gcaggccgct tttatctcgc caggttaata acgaaccggg catccagcgt
     9061 acgacagtaa atgtctctgg gtcggcttta agctgttcgc cattgattat gattttggtc
     9121 cgtgtacggg ctaacgtatc ccaggggcca aatggtacgg caacgcaggg gttaaacgtg
     9181 gtggaaaact ttcctcgctg atcccaggac ttctgcgcca gcacacggaa actctcgggg
     9241 tcagcgaggc gatgtccatc gacatataag aaattagcgt cacgtaatat cagccccaga
     9301 aagtcaggtt tccaggggga acggaatttc acctgatgca gcgttttaat atctagacta
     9361 ttttctccgc cattgtcatc ggtgcgcgtg ccttcaaaat agagactgtg tttatctgcg
     9421 gcaaaatcac cccatgcctt gaacgaggcg acatcgacgg gaggtttgtc tggcgggttt
     9481 tgcacaactt cacccgcgta gaggatatag cgaccatcgc tatgccagga gtaatgagga
     9541 taccaggcct gtgcgccttc atagaggtag tcggttgtca gatcagtcag aggaggtact
     9601 ttatcccatt tgtctagtgt tgtcgggtct attaataaat tgggtaaacg gcgcaaactt
     9661 ttgtaattca cgtcaggaag agaaagattt gtttctttat aagggcgcag gtataccacg
     9721 ccttttccct gtttctggta aatattatgt tcaggcgcgg aaagccgaca tccccatgcc
     9781 gacgataaaa acaacgaaca agacaacaaa caaaccacat tgcgaaagtg cataaagcca
     9841 tcctggcgcg aggtgttgat cacgaaactg ctagcagaac ataaatcctc acaagtaagc
     9901 gtaactctcg cagcaaatcg tcacttttca gaatccataa aggtcatcgc tattttctga
     9961 tagttgctgg aataaacgtg atattttcat tcacgatata aacacaatcc cggaactgcc
    10021 ccatagtacg gctaatagca taaaaatatt ccggattgac ccctggaagg gaggcaatgt
    10081 gggattccgc cccccgttgc cttctttatt gtctttcacg agttgtaccc aggttccacc
    10141 acgggtatct aacattcgcc acattattac gctttctgcc gaacgtgatt tttatagaaa
    10201 tctatcaatt cattttccct gtaccataaa gtttatcctt cacatcctgc cgaacgcggg
    10261 tcttgtaccc cccgttgtga agatcttcat acggagaaaa agaatcttta ctccacccca
    10321 gcaacacttt atcgccaggc ataaaatgca cgtgcaggta attttctccc cattctcttt
    10381 ttgggagagg taacgtcata ttgcgtttct ccagacgcat tccagcatta tactgtgtac
    10441 gagtagtact taatgtccat ataacctctg ccgtatcccc actgacatca ccgcaacagg
    10501 tgattgaacc gccaccattc gcaaaagcat tcgcgcccgc cacgccgtta acactgaatc
    10561 ccagtatcgg acggtcaatt tcactgtgga tttccagtat cactccacct cttggtcctc
    10621 cccatactgc ctgtcctata ttccacaaca cgaccagtac tggaacgaca attaacgtcc
    10681 gccataccca gcgcccccag cgggcataac cgcgcgtaag cttttggtca aatttttcca
    10741 gttgggtaaa tagccccatc gattttattc cctcttctca cctggttggc aaagttgcac
    10801 gtcactggcg actaaggtca gacaacgaaa tttcgtggta cgccactctc ttaaaagtcc
    10861 catgcaggcg ataagaacaa gacaacaaac aaaccacatt gcgatagtgc ataaagccat
    10921 cctggcgcga ggtgccgatc acgaaactac cagcaaaaca taaatcgcca cgtgtaagcg
    10981 ttatactcgc agcatttcct cacttttcag acttcataaa gagtcgctaa acgcttgctt
    11041 ttacgccttc tcctgcgatg atagaaagca gaaagcgatg aactttacag gcaatccata
    11101 atgaaaaacg tgtttaaggc actcactgta ttacttactc tcttcagcct gacgggctgc
    11161 ggtctgaaag gtccgctcta tttcccgcct gcagataaaa acgcaccgcc gccgaccaaa
    11221 ccggtagaga tgcaaacgca atccacggtg ccggataaaa acgaccgcgc cactggcgat
    11281 ggtccatccc aggtgaatta ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg
    11341 atgcagttct cgaaaatgca tggccttggc aacgatttta tggtcgtcga cgcggtaacg
    11401 cagaatgtct ttttttcacc ggagctgatt cgtcgcctgg cggatcggca cctgggggta
    11461 gggtttgacc agctgctggt ggttgagccg ccgtatgatc ctgaactgga ttttcactat
    11521 cgcattttca atgctgatgg cagtgaagtg gcgcagtgcg gcaacggtgc gcgctgcttt
    11581 gcccgttttg tgcgcctgaa agggctgact aataagcgcg atatccgcgt cagcaccgcc
    11641 aacgggcgga tggttctgac cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa
    11701 cccaacttcg aaccttccgc cgtgccgttt cgcgctaaca aagcggaaaa gacctatatt
    11761 atgcgcgccg ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa tccgcattgc
    11821 gtgattcagg tcgatgatgt cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa
    11881 agccacgaac gtttcccgga gcgcgccaat atcggtttta tgcaagtggt taagcgcgag
    11941 catattcgtt tacgcgttta tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc
    12001 gcgtgtgcgg cggttgcagt agggattcag caaggtttgc tggccgaaga agtacgcgtg
    12061 gaactccccg gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg
    12121 actggcccgg cggtacatgt ctacgacgga tttattcatc tatgaagcaa ccaggggaag
    12181 aactgcagga aacactcacg gagcttgatg accgggcggt tgtcgattat ctgattaaaa
    12241 atcctgagtt ttttatccgc aatgcgcgcg cagtagaagc gatacgtgtg ccgcatccgg
    12301 tacgcggcac cgtttcgttg gtcgagtggc acatggcccg cgcacgtaat cacattcatg
    12361 ttctggaaga gaacatggcg ttgttgatgg agcaggctat cgccaacgaa ggcctgtttt
    12421 atcgcttact ctacttgcag cgtagtctca ccgccgccag cagtctcgac gatatgctga
    12481 tgcgctttca ccgctgggcg cgcgatctcg gcctggcggg tgcgagcctg cgcctgttcc
    12541 cggattgctg gcgtttaggt gcgccgtcga accacactca tctggcatta agccgtcagt
    12601 ctttcgaacc gctgcgtatt cagcgtttgg ggcaggaaca gcactatctt gggccgctta
    12661 acggaccgga gctgctggtg gtgctaccgg aagcgaaagc ggtgggatcg gtggcgatgt
    12721 cgatgctggg aagcgatgct gatttgggtg tcgtgctgtt taccagtcgc gatgccagtc
    12781 actatcaaca agggcaggga acgcagttac ttcatgaaat tgcgctgatg ttgccggagc
    12841 ttctggagcg ttggattgaa cgcgtatgac cgatttacac accgatgtag aacgctacct
    12901 acgttatctg agcgtggagc gtcagcttag cccgataacc ctacttaact atcagcgtca
    12961 gcttgaggcg atcatcaatt ttgccagtga aaacggcctg caaagctggc agcaatgtga
    13021 tgtgacgatg gtgcgcaatt tcgctgtacg cagccgccgt aaagggctgg gagctgcaag
    13081 tctggcgtta cggctttctg cgctacgtag cttttttgac tggctggtca gccagaacga
    13141 actcaaagcc aacccggcga aaggtgtttc ggcaccgaaa gcgccgcgtc atctgccgaa
    13201 aaacatcgac gtcgatgata tgaatcggct gctggatatt gatatcaacg atcccctcgc
    13261 cgtgcgcgat cgggcaatgc tggaagtgat gtacggcgcg ggtctgcgtc tttccgaact
    13321 ggtggggctc gatatcaaac acctcgacct ggagtccggc gaagtgtggg tgatgggaaa
    13381 aggcagcaaa gagcgccgcc tgccgattgg tcgcaacgct gtggcgtgga ttgagcactg
    13441 gcttgatttg cgcgacctgt ttggtagcga agacgacgcg ctttttctgt cgaaactggg
    13501 caagcgtatc tccgcgcgta atgtgcagaa acgctttgcc gaatggggca tcaaacaagg
    13561 gctgaataat cacgttcacc cgcataaatt acgtcactcg ttcgctacgc atatgctgga
    13621 gtcgagcggc gatcttcgtg gtgtgcagga gctgctgggt catgccaacc tctccaccac
    13681 gcaaatctat actcatcttg attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc
    13741 acgcgccaaa cgggggaaat aatgcgtttt taccggcctt tggggcgcat ctcggcgctc
    13801 acctttgacc tggatgatac cctttacgat aaccgtccgg tgattttgcg caccgagcga
    13861 gaggcgctta cctttgtgca aaattatcat ccggcgctgc gcagcttcca gaatgaagat
    13921 ctgcaacgcc tgcgccaggc ggtacgggaa gcggaacccg agatttatca cgacgtgacg
    13981 cgctggcgtt ttcgttcgat tgaacaagcc atgctcgacg ccgggctgag tgccgaagaa
    14041 gccagtgcag gcgcacacgc agcaatgatc aactttgcca aatggcgcag ccgaatcgat
    14101 gtcccgcagc aaacccacga caccttaaaa cagctggcga agaaatggcc gctggtggcg
    14161 atcaccaacg gtaacgccca gccggagctg tttggtttag gggattattt tgagtttgtg
    14221 ttgcgcgctg gcccgcacgg gcgctcaaaa ccgttcagcg atatgtactt cctggcggcg
    14281 gaaaaactca acgtgccgat cggcgagatc ttacatgttg gggacgatct caccactgac
    14341 gtgggtgggg caattcgcag cggaatgcag gcctgttgga tcagaccgga aaatggcgat
    14401 ctgatgcaaa cctgggacag ccgtttactg ccgcatctgg aaatttcccg gttggcatct
    14461 ctgacctcgc tgatataatc agcaaatctg tatatatacc cagctttttg gcggagggcg
    14521 ttgcgcttct ccgcccaacc tatttttacg cggcggtgcc aatggacgtt tcttacctgc
    14581 tcgacagcct taatgacaaa cagcgcgaag cggtggccgc gccacgcagc aaccttctgg
    14641 tgctggcggg cgcgggcagt ggtaagacgc gcgtactggt gcatcgtatc gcctggctga
    14701 tgagcgtgga aaactgctcg ccatactcga ttatggcggt gacgtttacc aacaaagcgg
    14761 cggcggagat gcgtcatcgt atcgggcaac tgatgggcac cagccagggc ggcatgtggg
    14821 tcggcacctt ccacgggctg gcgcaccgtc tgctgcgtgc gcaccatatg gacgccaatc
    14881 tgccgcagga ttttcagatc ctcgacagcg aggaccagct acgcctgctc aaacgcctaa
    14941 tcaaggcgat gaacctcgac gagaagcagt ggccgccgcg ccaggcaatg tggtacatca
    15001 acagccagaa agatgaaggc ctgcgtccac atcatattca aagctacggt aatccggtgg
    15061 agcagacctg gcagaaggtg tatcaggcgt atcaggaagc gtgtgaccgc gcgggcctgg
    15121 tggacttcgc cgagctgctg ctacgtgctc acgagttgtg gcttaacaag ccgcatatcc
    15181 ttcagcacta tcgcgaacgg tttaccaata tcctggtgga tgaattccag gataccaaca
    15241 acattcagta tgcgtggatc cgcctgctgg cgggcgatac cggcaaagtg atgatcgttg
    15301 gcgatgacga ccagtcaatc tacggctggc gtggggcgca ggtcgagaat attcagcgtt
    15361 tcctcaatga tttccccggt gctgaaacta tccgtctgga gcagaactac cgctctacca
    15421 gcaatattct gagcgccgct aacgccctga ttgaaaacaa taacgggcgt ctgggtaaaa
    15481 aactgtggac cgatggcgcg gacggtgagc ctatttccct ctattgcgct tttaacgaac
    15541 tcgacgaagc gcgttttgtg gttaaccgta tcaagacctg gcaggacaat ggcggggcgc
    15601 ttgccgagtg cgccattctc taccgcagca acgcccagtc gcgtgtactg gaagaagcac
    15661 tgttgcaggc gagtatgccg taccgtattt acggcgggat gcgcttcttc gaacgccagg
    15721 aaatcaaaga tgcgctctcg tatctgcgcc tgattgccaa ccgtaatgac gacgcggcgt
    15781 ttgagcgcgt ggtgaataca ccaacgcggg gtattggtga ccggacgctg gacgtggtac
    15841 gtcagacatc gcgcgatcgc cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg
    15901 aaaaagccct cgccggacgt gctgccagtg ccttacagcg gtttatggaa ctgatcgacg
    15961 ccttagcgca ggaaactgcc gatatgccgc tgcatgtaca gactgaccgg gtaattaaag
    16021 actccggcct gcgcacaatg tacgagcagg agaaaggcga aaaaggccag acgcgtatcg
    16081 aaaacttaga ggaactggta acggcgacgc gccagttcag ctacaacgaa gaagacgaag
    16141 atttaatgcc gctgcaggcg ttcctctccc atgcggcact ggaagcgggc gaggggcagg
    16201 cggatacctg gcaggatgcg gtgcagttga tgacgctaca ctcggcgaaa ggtctggaat
    16261 tcccgcaggt gtttatcgtt ggtatggaag agggcatgtt cccaagccag atgtcgctgg
    16321 atgaaggcgg gcgtctggaa gaagaacgcc gtctggccta cgttggcgta acccgcgcga
    16381 tgcagaaact gacgctgacc tacgcggaaa cccgccgtct atatggcaaa gaggtttacc
    16441 atcgcccgtc gcgctttatc ggtgagttgc cggaagagtg tgtggaagag gtgcgcctgc
    16501 gcgccacggt aagccgcccg gtcagccatc aacggatggg gacgccgatg gtcgagaacg
    16561 acagcggcta caagctcggc cagcgcgtac gccacgctaa gtttggtgaa ggcaccatcg
    16621 tcaatatgga aggcagcggt gagcatagcc gtttgcaggt ggcgttccag ggccagggta
    16681 ttaaatggct ggtggcggct tacgcccggt tggagacggt gtgacgttgc gggatgcggt
    16741 gcgccacacc gcattccgca tcagcacctt acttagcgca cttcttcaca tcaatattcc
    16801 agtcagcacc agacgcaacc tgatgtttct gcgcaaaatt atccatattc agcgccacgg
    16861 aaacattcaa taagctgttc agatacacca tcggctggcc ttccggcaca tcgccgaagc
    16921 ttgcgacata cggcattttc cctgcgtatt tttgctggga accttcggaa atggtcacac
    16981 acaacgtgtc gttgagtttg attcctgcct gtttcagcaa ctcgtcgctg atattgctcc
    17041 agacgttgcc gtactgaata tcgagaatcg ggatattgcc cttcacttca ccttttgttg
    17101 cttttgcttt ctgataagaa agttcgacaa cttttgcagg aagctctggc cccacctgtt
    17161 cgaacgtaat cgctccggaa gccagacgcg caccagtgta ggcatataca tcgcgaccat
    17221 ggaaggtata tgacttttca gaacctttca ggcggttagc tttttcgtcg atttcccgga
    17281 cgctatcaat acccagagtt tgtgccacca aagtgagcgt accgttatct ggtgaaacga
    17341 agtattgacc gtttttggtt ttcagcacta ccgatttacg gtcagtgcct acgcccggat
    17401 cgaccacgct aacaaacacc gaaccttgcg gccagtaact ggcagtctga tacaggcgat
    17461 aagcgccttc ccagatgtta tacggtggaa tttcgtgtgt taaatcaaag attttaagat
    17521 tatggtcaac gccgaatgcg acccctttca tcgccgagac ggcaccatct ttcaggctaa
    17581 aatccgtttg taaaatcaaa gcgttatccg cataagcgga tgatgacccc agacaggtta
    17641 ataaaaataa tgttgcgagc gtttttttca tttttgtttc ccttcattta tctgatgatt
    17701 ttccagctgt gtagccagcc ccagtttgtc tggcctcctc gagtgtacaa tgtttaacct
    17761 ttctgctcga cggaaatcat ggtgacaaaa ggatagcgtt gccacgggat tgcctcgcct
    17821 ttcatataca tatgtgaaat tgtgccatca agataaagca gctgcccaac gttcagtttc
    17881 gctttggcat aacaggcaaa atcataaaaa tttgtcgcct gctggctcaa caaaaacacg
    17941 gcgttcccat gtttattaat cccaacaccg ttacgaattt tacgtgaggc gacgttggga
    18001 tgaatacgcg gattaattac accgttttcc atcaacaatg gccctgactg caccgcaaac
    18061 tgaatctctt tactggtttt aaaggcatcc agacgaacga tgccgacttt atctcccgcg
    18121 acataaaaca ctccgccagg acgaataaag aaattccctt cacctgaagc gagatttaac
    18181 gccaccttct gctgaccgtt ttcgatgtac aaaccgagcg gcgcatagct ttcatcatag
    18241 atgccgccgt tcatcgccat ctgcacctga ccctgactat taatatccgc cagcagagca
    18301 tgtaacgttc cccacgcttc gccattggct ttttgccagt acattttcac ccgctctgtt
    18361 tgaggattaa cggtatacgc ctgtacggtc agcgtcggat ctgagggtgc gcaatcatca
    18421 gcggcaacag caaacaaggg aagtaaggtg agggtgagaa aaatccgttt gagattcaag
    18481 gtgatcattc ctttaccaat gaatagccga tgcgccatta taggccctgg atgtgggatt
    18541 tttttatcct gttagcgacc ttgacgagta ccaaaaagcg cgaagttcaa ctattgttct
    18601 gtggtgttct gttgcgtgtt gacggcaaaa ttttgctggc gtaacatgcg cgcacgatca
    18661 ctctaagagg acattcgcct tggacacacc cagtagatac tggctcacta tcctgtcatc
    18721 caggatcaac tcctaaggct atcccttttt gctgatagcc ttagcggttg tcagcgacct
    18781 caatttttcc cgtcgcgctg agtcaggctg tttaatggtc tgaaacccaa tttgtttctg
    18841 tgtgcccacc gaactgtccg atattttaag cattgggagt cccggtcatg ctgagcgcat
    18901 ttcaactgga aaataaccga ctgacccggc tggaagtcga agagtcacaa ccccttgtaa
    18961 atgcagtatg gattgatctt gtcgaaccgg acgacgacga gcgactgcgc gtacaatctg
    19021 aacttggtca gagcctggca acccgcccgg aactggaaga catcgaagca tcggcacgtt
    19081 tctttgaaga cgatgacggc ctgcatattc actccttctt cttctttgaa gatgcggaag
    19141 atcacgccgg taactctact gtggcattta ccatccgtga tggtcgtctg tttactctgc
    19201 gtgagcgtga actgcctgca ttccgtctgt atcgtatgcg cgcccgtagc cagtcaatgg
    19261 tggacggtaa cgcctacgag ctgctgttgg atctgttcga aaccaaaatc gaacagttgg
    19321 cggatgaaat tgaaaacatc tacagcgatc tggagcagtt gagccgggtg attatggaag
    19381 ggcatcaggg cgatgagtac gacgaggcgc tctccacgct ggcggaactg gaagatatcg
    19441 gctggaaagt tcgcctgtgt ctgatggata cccagcgtgc gctcaacttc ctggtacgta
    19501 aagcacgctt accgggtggg caactggagc aggcgcgtga aatcctgcga gatatcgaat
    19561 ccctgctgcc gcataacgaa tccctgttcc agaaggtgaa cttcctgatg caggcggcga
    19621 tgggctttat caatatcgag cagaaccgca tcatcaaaat cttctcggtg gtatccgtgg
    19681 tgttcctgcc accgacgctg gtcgcttcca gctacgggat gaactttgag tttatgccgg
    19741 aactgaagtg gagcttcggc tacccaggcg cgattatctt tatgatcctc gcgggcctgg
    19801 caccgtatct gtactttaag cggaagaact ggttgtaata taaatgccgg gtaaagattg
    19861 ttctttatcc ggcttattgc tgaaaattgt ctttttcaaa gtcatgcggg ttgaacactc
    19921 gctctatctt attttcctgg
//
LOCUS       DAZGAP010000098        19856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGAP010000098 DAZGAP010000000
VERSION     DAZGAP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.098"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..359
                     /gene="pduA"
                     /locus_tag="ACHLAM_002992"
     CDS             75..359
                     /gene="pduA"
                     /locus_tag="ACHLAM_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFO7854486.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            356..1168
                     /gene="pduB"
                     /locus_tag="ACHLAM_002993"
     CDS             356..1168
                     /gene="pduB"
                     /locus_tag="ACHLAM_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFO7854487.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1187..2851
                     /gene="pduC"
                     /locus_tag="ACHLAM_002994"
     CDS             1187..2851
                     /gene="pduC"
                     /locus_tag="ACHLAM_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFO7854488.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2861..3529
                     /locus_tag="ACHLAM_002995"
     CDS             2861..3529
                     /locus_tag="ACHLAM_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFO7854489.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKPVSFRAPVSATASSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3544..4062
                     /gene="pduE"
                     /locus_tag="ACHLAM_002996"
     CDS             3544..4062
                     /gene="pduE"
                     /locus_tag="ACHLAM_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFO7854490.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4072..5904
                     /locus_tag="ACHLAM_002997"
     CDS             4072..5904
                     /locus_tag="ACHLAM_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFO7854491.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSYVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     LGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKGAIHGK"
     gene            5894..6244
                     /locus_tag="ACHLAM_002998"
     CDS             5894..6244
                     /locus_tag="ACHLAM_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFO7854492.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHLAM_002999"
     CDS             6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHLAM_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFO7854493.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6563..6985
                     /locus_tag="ACHLAM_003000"
     CDS             6563..6985
                     /locus_tag="ACHLAM_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFO7854494.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6988..7620
                     /locus_tag="ACHLAM_003001"
     CDS             6988..7620
                     /locus_tag="ACHLAM_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFO7854495.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVTVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVT"
     gene            7617..8108
                     /gene="pduM"
                     /locus_tag="ACHLAM_003002"
     CDS             7617..8108
                     /gene="pduM"
                     /locus_tag="ACHLAM_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFO7854496.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHDRLLSYVDIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8112..8387
                     /locus_tag="ACHLAM_003003"
     CDS             8112..8387
                     /locus_tag="ACHLAM_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFO7854497.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRQVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8399..9406
                     /gene="pduO"
                     /locus_tag="ACHLAM_003004"
     CDS             8399..9406
                     /gene="pduO"
                     /locus_tag="ACHLAM_003004"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFO7854498.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHCFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9403..10788
                     /gene="pduP"
                     /locus_tag="ACHLAM_003005"
     CDS             9403..10788
                     /gene="pduP"
                     /locus_tag="ACHLAM_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFO7854499.1"
                     /translation="MNNSELETLIRNILREQLVPATQRAQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VVSVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10799..11911
                     /locus_tag="ACHLAM_003006"
     CDS             10799..11911
                     /locus_tag="ACHLAM_003006"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFO7854500.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIHFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11908..13251
                     /locus_tag="ACHLAM_003007"
     CDS             11908..13251
                     /locus_tag="ACHLAM_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7854501.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVNVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGNLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13254..13808
                     /gene="pduT"
                     /locus_tag="ACHLAM_003008"
     CDS             13254..13808
                     /gene="pduT"
                     /locus_tag="ACHLAM_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFO7854502.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13808..14158
                     /gene="pduU"
                     /locus_tag="ACHLAM_003009"
     CDS             13808..14158
                     /gene="pduU"
                     /locus_tag="ACHLAM_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFO7854503.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14163..14606
                     /locus_tag="ACHLAM_003010"
     CDS             14163..14606
                     /locus_tag="ACHLAM_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO7854504.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14707..15036)
                     /locus_tag="ACHLAM_003011"
     CDS             complement(14707..15036)
                     /locus_tag="ACHLAM_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO7854505.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15208..16266)
                     /locus_tag="ACHLAM_003012"
     CDS             complement(15208..16266)
                     /locus_tag="ACHLAM_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7854506.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="ACHLAM_003013"
     CDS             complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="ACHLAM_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO7854507.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="ACHLAM_003014"
     CDS             complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="ACHLAM_003014"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO7854508.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVAMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18431..>19856
                     /gene="sbcB"
                     /locus_tag="ACHLAM_003015"
     CDS             18431..>19856
                     /gene="sbcB"
                     /locus_tag="ACHLAM_003015"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO7854509.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSKFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
BASE COUNT         5071 a         5140 c         4993 g         4652 t
ORIGIN      
        1 cgattaagtc agccattctt tttcctcgct tttttttata gtcatatcgt caaaaaaatc
       61 caggcgaggt ctttatgcaa caagaagcgt taggaatggt agaaaccaaa ggcttaactg
      121 ccgccataga ggccgcagac gccatggtga agtcggcaaa tgttttgctg gtcggctacg
      181 aaaaaatagg ttccgggctg gtgaccgtca tcgttcgtgg tgatgtcggg gcggtaaaag
      241 cggcaacaga cgcgggtgcc gcagctgcgc gtaacgtcgg cgaagtaaaa gccgtacacg
      301 ttattccccg cccacatacc gatgtcgaaa aaatcttacc aaagggaatt aacccatgag
      361 cagcaatgaa ctggtcgaac agatcatggc gcaggtcatc gctcgcgtag cgacgccaga
      421 gcatgaggtt ctcccgaaaa ccactcatca aatacgagag acggctatgg cagaaaaaag
      481 ctgcacgtta acagaatttg ttggcaccgc tattggcgac actgtaggtc tggtcattgc
      541 gaacgttgat agcgccctgt tggacgcgat gaaacttgaa aaacgctatc gttccattgg
      601 cattctgggg gcgcgtattg gcgcagggcc acacattatg gccgctgatg aagcggtaaa
      661 agccaccaac actgaagtta ttagcattga acttcctcgg gatactaaag gcggtgccgg
      721 acacggctca ttgatcctct taggtggcaa cgatgtttcc gacgtgaaac gcggtattga
      781 agttgccctg aaagaactcg atcggacctt tggtgatgtc tatgccaacg aagccgggca
      841 tcttgaactg caatacaccg cccgcgccag ctatgcactg gaaaaagcgt ttggcgctcc
      901 actgggacgg gcttgcggcg ttattgtcgg cgctccggct gcggtgggtg ttctgatggc
      961 cgacaccgcg ctgaagtccg ccaatgtgga tgtcgtggca tacagctccc ccgctcaagg
     1021 caccagctac agcaacgaag tcattctgat tatctcgggt gactccggcg cagtccgcca
     1081 ggcggtcatc tccgcacgcg aaatcggtaa aaccgtgctt tcgactctcg gcgatacacc
     1141 gaaaaacgat cgcccttcct acatctgaat tctctgaggc agatttatga gatcgaaaag
     1201 atttgaagca ctggcaaaac gcccggtcaa ccaggacggt ttcgttaaag agtggattga
     1261 agaaggcttc attgcgatgg aaagcccgaa cgatcctaaa ccgtcaatca aaattgttaa
     1321 cggcgtcgtt accgaactgg atggaaaacc cgccaatcag tttgacctga tcgaccactt
     1381 tatcgcccgc tacggcatca atctcgcgcg cgcagaagaa gtcatcgcga tggattcggt
     1441 aaaacttgcc aatatgttgt gcgatccaaa tgtcaaacgt agcgatatcg ttccccttac
     1501 cacagcaatg acgcctgcaa aaatcgtcga agtggtttcg cagatgaacg tggtagagat
     1561 gatgatggcg atgcagaaaa tgcgcgcccg tcgaacacca tcacaacagg ctcacgtcac
     1621 caacatcaaa gataacccgg tacaaattgc cgccgacgca gctgaaggcg catggcgcgg
     1681 gtttgatgaa caggaaacca ccgttgccgt tgctcgctat gcaccgttca acgccatcgc
     1741 cctgctggtc ggctcacagg tgggacgtcc gggcgtcttg acccagtgtt cactggaaga
     1801 agccaccgaa ctgaaactcg gtatgttagg ccacacctgt tacgccgaaa caatttctgt
     1861 ctatggtaca gaacctgttt tcaccgatgg cgatgatacc ccgtggtcaa aagggtttct
     1921 cgcgtcatcc tatgcgtctc gcggcttaaa aatgcgcttt acttccggct ccggctctga
     1981 agtacagatg ggttacgcgg aaggcaaatc aatgctttat ctcgaggctc gatgcattta
     2041 catcaccaaa gccgcaggcg ttcagggtct gcagaatggt tcggtaagct gtatcggtgt
     2101 tccatcagct gtgccatcag gcatccgcgc agtattagcg gaaaacttaa tttgctcatc
     2161 gctggatctg gaatgcgcct ccagtaatga ccagaccttt acccactcgg atatgcgccg
     2221 taccgcacgt ttcctgatgc aatttctgcc aggtaccgac tttatctctt ccggtttttc
     2281 cgctgtgccg aactacgaca acatgtttgc tggttcaaac gaagatgctg aagacttcga
     2341 tgattacaac gtcattcagc gtgacctgaa ggttgatggc ggcctgcgac cagtacgcga
     2401 agaagatgtt atcgctatcc gcaataaagc cgctcgcgct ttacaggctg tctttgccgg
     2461 tatgggacta ccgcctatta ccgatgaaga agtcgaagcg gcaacctatg cccacggttc
     2521 aaaagatatg cctgagcgca atattgtaga agacatcaag ttcgcccagg agattatcaa
     2581 taaaaaccgg aacagcctgg aagtggtgaa agccctggcg caaggcggtt ttaccgatgt
     2641 cgcccaggac atgctcaaca tgcaaaaagc aaaactgacc ggcgattatc tccatacctc
     2701 agccatcatc gtcgatgacg gacaagtgct ctctgcggtc aatgacgtca atgattacgc
     2761 cggaccggct acaggttacc gcctgcaagg ggaacgctgg gaagagatca aaaatattcc
     2821 cggtgcactt gatcccaacg aaattgacta agggtgagaa atggaaatta acgaaaaatt
     2881 actgcgtcag attatcgaag acgtgctggc agaaatgcaa accagcgaca agcccgtttc
     2941 atttcgcgca cctgtctctg caactgcctc ttccgcgcca gataccggaa attttctgac
     3001 cgaaattggc gaagcccaac aaggcactca gcaagatgaa gtcattattg ctgtaggccc
     3061 ggcgttcggc ctggcgcaaa ccgtcaatat catcggtatt ccgcataaaa acattttgcg
     3121 cgaagtgatt gccggtatcg aagaagaagg cattaaagcc cgtgtgattc gctgctttaa
     3181 atcgtccgat gtcgcattcg tagcagtcga aggcgatcgt cttagtggtt caggtattgc
     3241 tatcggtatt cagtcgaaag gcacaacggt tatccaccag cagggacttc ctcccttgtc
     3301 taacctggaa cttttcccac aagcgccttt gttgacgctg gatacttatc gtcagattgg
     3361 caaaaacgcc gcgcgttatg caaagcgtga gtctccacaa ccggtgccga ccctcaacga
     3421 tcaaatggcg cggccgaaat accaggccaa atctgccatt ttgcatatta aagagacgaa
     3481 gtacgtggtc accggcaaaa aaccgcaaga actgcgcgtg acgttttaac aaaggaaacc
     3541 ttcatgaata ccgatgcaat tgagtcgatg gtcagagacg tgttgaaccg catgaacagt
     3601 ttacaggatg cagctcctgt ttctgcggtc cccaacgcgt caatacagag cgccaaagtg
     3661 acggattacc cgctggcaaa taaacatccg gaatgggtta aaacggcgac caataaaacg
     3721 ctcgacgatt tcactctgga aaatgtgcta agcgataacg tcaccgccca ggatatgcgt
     3781 ataacccctg aaacattgcg tattcaggcc gcaattgcca gagatgctgg tcgcgatcgc
     3841 ctggcgatga attttgaacg cgccgctgag cttacctcag taccggatga tcgcatcctc
     3901 gaaatctaca atgcgttacg cccttatcgt tcgactaagc aagagcttat tgccattgct
     3961 gacgatcttg aacggcgtta tcaggccaaa atttgtgctg catttgtccg tgaagctgcg
     4021 gaactttatg tcgagcgcaa aaaactgaaa ggcgacgatt aaggacgaac tatgcgatat
     4081 atagcaggca ttgatatcgg caactcatca acagaggttg cgctggcaac gctcagtgca
     4141 actggagagt tgtcgtacgt gagtagcgcc ctggcggaaa ccaccggaat taaaggtacg
     4201 ttgcgtaacg tccatggtat tcaggaggcg ctcgcacagg caacgaaaaa agtcggcatc
     4261 aatgttagcg atatttcgct tattcgtatc aacgaagcaa ctccggtcat tggtgatgtg
     4321 gcgatggaaa ccatcactga aaccatcatc accgaatcaa ccatgatcgg tcacaacccg
     4381 aaaactccgg gcggagttgg gctgggcgta gggctcacca tcactccgca agagttatta
     4441 acacgcccgg cggacacacc ttacatcctg gtggtgtcat cggcatttga ttttgccgat
     4501 atcgcaacga tgataaacgc ctccgtacga gccggatatc agctgactgg cgttattctg
     4561 caacgcgacg atggcgtact ggtcaataac cgcctggaaa ttcctctccc tattgtggat
     4621 gaagtcctct acatcgatcg tattccgctc ggcatgctgg ctgcaattga agttgccgtt
     4681 cccgggaaag ttatcgaaac cctgtctaac ccgtatggga ttgccaccgt tttcgccctc
     4741 aatgctgaag agactaaaaa tattgttcct gtcgcgcggg cgctaatcgg caaccgttca
     4801 gcagtggtag tgaaaacgcc ttctggcgac gttaaagccc gttcgatacc cgccggaaat
     4861 atcgaactgc tttctgccgg gcgcacaacg cgggttgatg tagctgctgg tgccgatgcc
     4921 attatgaaag cggtcggtga gtgcccaaaa ctggaaaacg tgacgggaga acctggctcc
     4981 aacatcggcg gaatgctgga acatgttcgc cagaccatgg ctgaactgac caacaaaccc
     5041 agcaacgaaa tttttattca ggatttgctg gcgatagata cctctgtccc cgtcaacgta
     5101 accggtggtc tggcaggtga attttcactg gaacaggctg tgggtattgc ctctatggtg
     5161 aaatccgacc gtctgcaaat ggcgatgatc gccagtgaaa ttaagcaaaa actgcatgtt
     5221 gatgttcagg tcggaggcgc agaagcagaa gccgccattc agggggcctt gactacccct
     5281 ggaaccacac gtccactggc aattctggac ttaggtgcag gctctaccga tgcctccatc
     5341 atcaatcaga aaggggaaat agttgccact caccttgcgg gagcgggcga catggtaacg
     5401 atgattatcg ctcgtgagct gggcttaaat gatcgttatc tcgcagaaga aatcaaaaag
     5461 tacccgttgg caaaagtaga aagcctgttc cacttacgcc atgaagatgg cagcgttcag
     5521 ttcttcccta ctccgctgtc ccctcatgta tttgcccgcg tttgtgtggt gaaacctgac
     5581 gaactggtgc ctattctcgg tgatttgacg ctggaaaaag tacgtgctgt ccgtcgtagt
     5641 gcgaaagagc gtgtcttcgt aactaatgcc ctgcgcgcgc tacgtcaggt aagccctgcc
     5701 ggaaatattc gcgacattcc gttcgtcgtt ctggtgggtg gttcttcgct ggatttcgaa
     5761 gtaccgcaat tggtcaccga cgcgctggca cattatcgac tggtcgccgg gcgggggaat
     5821 atccgtggta gcgaaggtcc gcgcaatgcg gtagctactg ggctcctcat cgcctggcac
     5881 aagggggcga ttcatggaaa gtaaagagcg tgcaccagcc atcgttgtca tggagatcgg
     5941 tgactgtatc actacatggg atgaagtgct tcttggcatt gaagaggaag gtattccctt
     6001 tcgcattcaa cacattccgt caggtgaagt tatcgatagc gcctggctgg cggctcgtca
     6061 atctcccctg ttggttggca ttgcctgcga tcaggaaaaa ctgattgtgc attacaaaaa
     6121 cttaccggca tcagcgccac tgtttacgct gatgtatcaa caagataacc atgccaggcg
     6181 cagcatcggc actaacgctg cccgcctggt aaaagggatt cccttccggg aactccactc
     6241 ataatccaca ggagaaacgc agtatgaaca acgcactagg actggttgaa acaaaagggt
     6301 tagttggcgc aattgaagct gcagatgcca tggttaaatc cgccaacgtg cagctggttg
     6361 gctatgaaaa gattggttcg ggtctgatta cggtgatggt ccgtggcgat gttggcgcag
     6421 taaaagccgc cgttgatgca ggaagcgccg ccgcaagcgc cgttggcgaa gtgaaatcct
     6481 gccatgtcat cccgcgccca cacagtgatg ttgaggctat tttacctaaa tcagcataac
     6541 cccattcgct aaggagcaca gcgtgaagca atcactggga ttacttgaag tcagtggtct
     6601 ggcactcgcc atcagttgcg cagatgctat ggcaaaagcc gcgtccatca cgctggttgg
     6661 ccttgagaag accaatggct caggctggac gctcatcaaa atcaccggtg acgtcgcctc
     6721 ggttaatgcg gcgatcgcga cgggagccag tttcgccggg cagcgaaatg gccttgtcgc
     6781 acacaaagta attgcccgcc cgggagacgg cattctgccc cacacaactg aagcattgac
     6841 tgtgacggtt gaaacaagcg aaacaccagc aacaaccgtc atctcagaga ttatcagttg
     6901 taatttgtgt cttgacccgg catgtcagcg gcaaaaaggt gagccccgct cactttgcct
     6961 gtacgccaat aagcgagggg agtagcagtg gataaacaaa ccctgcagga aacagtgagc
     7021 cagattctgg atgagatgcg cgagcgccct atcccactgg gcgtttccag tcgccatatt
     7081 catctttcaa cagcggatta tttgcgtctt tttcctgaac aaccgattag cgagaaaaag
     7141 ccgttaatgc aaccgggcca attcgctgct gaacaggttg ttacgctgga gggaccaaaa
     7201 ggcaaattgc acaacgttcg cttgttgggg ccgctacgca atgcaagcca ggtggaaatt
     7261 tcccggacgg acgccagaac gttagggatc accgccccgc tacgcatgtc aggcgatctc
     7321 agcggcactc cggggattcg tttgataagc ccatttgcgg aactggagat cacatctggc
     7381 gtcattgtcg cgcaacggca tattcatatg tctccgctgg atgccctgat ttatggtgtt
     7441 acgcacggcg ataaagtggc ggttgccatt gaaggtgatg agcgacgact gatattcgaa
     7501 aacgtcactg ttcgcgtctc tccggatatg cgtcttgaaa tgcatattga taccgatgaa
     7561 gccaacgccg caggggctga tagccctgac gtttttgcca cactggtgtc agtgacatga
     7621 ataacatgct gatgcaacgt attgttgacg aagtggtttt tcgccttaaa caacgtgcgg
     7681 gaaaaacact cgtgctgact gtttttcaac tgcgagacgc cagcgttcaa gagagcgttc
     7741 atcagtatgc gtcattacaa atccgatatg tcgatctgcc gctgctgcga caacttgcgg
     7801 agaacgaaac gtcagatcga gcagcaatcc agatacacga agcgttagcc tggggaatgc
     7861 acattcaatt gtcgttgcag cgtcatttcc tgaacgccat tgaattgaag actctcgccc
     7921 gtctaccgct aagctggtgc gacgaacaag ggcaaccgat ctacctacat catgatcgac
     7981 ttttaagtta tgtcgatatc gcacagttaa gctcgggaat attggtgtta cagcggaaat
     8041 gttgtgtgac cgcactggct cgtgaagcgg caattacccg aaatattcag ttaatcaggc
     8101 aggagtaacc tatgcatctg gctcgagtca ctggcgcggt agtatccacg caaaaatcac
     8161 cttctctgat cgggaaaaaa ctgctgctgg ttcgccaggt cagtgctgac ggcgaactgc
     8221 ctctcacgcc attaaccggc gacgaagttg ccgttgattc cgtcggcgca ggcgttggtg
     8281 agttggtact cctcagcagt ggttccagcg ccagacatgt gttttccggc cctaacgaag
     8341 ccatcgacct ggcagtggtc ggcattgtcg atacgctttc gcgctaagga cctgaaagat
     8401 ggcaatttat acccgaacag gtgattcagg aagtacgtct ttatttaccg gacaacgcgt
     8461 cagcaaaact catctacgtg tcgaaaccta tggcacgctg gatgaactga acgcgacact
     8521 cagtctgtgt tattgcgcca ccgccatcga aagccatcgc atcttactgg aagccattca
     8581 acaacagata ttttggttta gcgccgaact cgccagtgaa agcgaacagc cgtcagcgca
     8641 gcagcgttac atcggtacgg aagaaattgc cgcgctggaa aacgcgatcg atagcgcaat
     8701 gaacgccgtc cccccggttc actgcttcat tctgcctggt cgatgtgaag ccgcaagccg
     8761 catgcatttc gcccgcacgg tggctcgacg ggctgaaaga cgtctggttg aactgacaac
     8821 agaaaccacc gtccggaatg ttttgctgca ctatatcaac cgactttccg attgcctgta
     8881 tgccctcgcc cgcgtggaag acaacgttgc tcatcaaaac ctgatgattc aggaaatcac
     8941 aaaacgttat cacgaagcca accacacacc agcattgaag gaacgcacaa tgccgctcac
     9001 ttttcaggat cttcaccagc tcattcgtag tgcggcaatg cgcgcagatg aactgcatat
     9061 tcccgttgtc atcagcattg tcgatgccaa cggaacagaa agtgttacct ggcggatgcc
     9121 tgatgccctg ttggtcagca gtgaactggc accgaaaaaa gcctggaccg ccgtggcgat
     9181 gaaaacggca acccacaagc ttgctgatac cgtgcaaccc ggcgcgccgc tttatgggct
     9241 cgaaagccat atgcaaggca aagtcgtcac ctttggtggc ggcttccccc tctggcgtga
     9301 cggaaaattg cttggcgggc ttggcatcag cggcggtagc gttgaacaag acatggatat
     9361 tgcccaaagc gcaatggcgg caattaacgt gggagtaaac caatgaataa ctctgaatta
     9421 gaaacactga tccgcaatat tctgcgtgag cagcttgtcc ccgctacgca acgagcgcaa
     9481 agaaacgcta ttttccagac tgttgatgaa gcggtctgcg cagcacacca ggccttttta
     9541 cgctttcaac agtgcccgct taaaacccgt agcgccatta tcagcgccat tcgcgaagaa
     9601 ttaatgcccc gccttaccga actggcagaa gaaagcgcca aagaaacggg gatgggaaat
     9661 aaagaagata agtacctaaa aaacaaagcg gcacttgata acacgcctgg aattgaagat
     9721 ttaaccacca cagcactgac cggtgatgga ggcatggttt tgtttgaata ctctccgttt
     9781 ggggtgattg gctcggtcac gccaagcacc aaccccacgg aaaccatcat taacaacagc
     9841 atcagcatgc tggcagcagg taatagcgtc tacttcagtc cacacccggg agccaaaaac
     9901 gtttcgttaa aactgattac cataatcgaa gacatcgcgt ttcgctgtag tggcattcac
     9961 aacctgatcg ttaccgtatc tgaaccaacc tttgccgcaa ccgaacaaat gatggcgcac
    10021 cctaaaatca gcgttctggc gattaccggt ggcccgggga tcgtcgccat gggaatgaaa
    10081 agtggcaaga aagtgattgg tgccggagcg ggtaacccac cctgcattgt cgatgaaacg
    10141 gcggatattg tgaaagccgc cgaggacatc atcaacggcg cttcatttga ttacaacctg
    10201 ccgtgtatcg ctgaaaaaag cctgattgtc gtggtatcag tggcagagta tttaattcag
    10261 cagatgcaaa gctttggagc acttcttctt aaccatgaac aaacggagaa attgcgtgca
    10321 atttgcctgc cagacggtag cgccaacaaa aaactggttg ggaaaagccc atcagccttg
    10381 ctggaagcag cgggcctccc tttgcccgca aaagcgccgc gcctactgat tgctgttgtt
    10441 gacgccgatg atagttgggt tacgtgtgaa caactgatgc caatgctgcc cattgtgaag
    10501 gtcaacgatt ttgacagcgc tctcgccctg gcattgaaag tggaagacgg cttgcaccac
    10561 acggcaatta tgcactcgca gaacgtctca cggctcaacc ttgccgcgcg cgttatgcaa
    10621 acctcaatct ttgtcaaaaa cggcccctcc tgggcgggta ttggcgtcgg cggcgaaggc
    10681 tttacgacct ttaccattgc aacaccaacc ggagagggta caacgtcagc acggactttt
    10741 gcccgctcac gacgctgcgt gttaaccagc ggcttttcca ttcgctagag aaacggaaat
    10801 gaaaactttt tcactgcaga cgcgtttata cagcggaacg ggaagtctca acatactgaa
    10861 ccgtttcacg aataggcaca tctggattat ttgcgacgtc tttctggcac actcaccact
    10921 cattgacacg ttgcatcagg cgctgcccga cagtaatcgt ctcagtattt ttagcgaaat
    10981 aacgcccgac ccaactatcc agaccgtggt gaaaggcatt gcgcaaatgc agactttacg
    11041 cccggatgtg gttatcggtt ttggcggcgg ttctgccctg gacgccgcaa aagcgattgt
    11101 ctggttcggt cgccagtgtg gcattgaaat tgaaacctgc gtagccattc ctaccaccag
    11161 cggtaccggt tccgaagtga ccagtgcttg tgtcatcagc gatcctgaga aagggattaa
    11221 atatcccctt ttcgataatg cactttatcc ggatatcgcg atccttgatc catctttggt
    11281 cgtcagcgtg ccaccggcga taacggccaa taccgggatg gatgttttaa cccatgcgct
    11341 tgaagcttat gtttctcccc gcgccagcga cttcacggat gcactggtcg aaaaagccgt
    11401 gcaaattgtt tttcaatacc tgcccactgc agtgaagaaa ggcgattgtc ttgccacccg
    11461 tggcaaaatg cataatgcat cgacgcttgc aggaatagcc tttagccagg ccggactcgg
    11521 tttaaaccat gccattgctc atcaactcgg cggacagttt catctgccgc acggcctggc
    11581 gaatgcgctc ctgctcacgg cggttatcca ctttaatgct ggcgatccgc gcgcagccaa
    11641 acgctacgcc cggctggcga aaacgtgcca tttatgcccg gacaacgcca atgacaccgc
    11701 cagcctgaat gcacttattc aacatattga gcagcttaaa acaacctgta cgctgccgac
    11761 tcttaccaat gcgctgaaag aaaaaaaagc agaatggtca atacgcatac cagatatggt
    11821 tcaggcggca ctggcggatg caacgttgcg taccaacccg cgtgcggctg atgcctccgc
    11881 tattgcagaa ctgctcgagg agttgttatg aacgtcgagt taacctcctg cgacgaccag
    11941 accatccgcg agcgagtaca ggcagcaggt gtcgtgggtg ctggcggcgc aggttttcca
    12001 acccatatta agctacaagc gcgtgttgac acttttctgg tgaatgcggc ggaatgcgaa
    12061 ccaatgttga aagtggatca acagttaatg gtgcagcaag ccagccgcct gcttcgcggt
    12121 gttcactatg cgatgaaagc caccggggcc agtagcggaa tcatcgcgct gaaagagaaa
    12181 tatcaacggg caataaacgc actcaccccc ctattaccac cggatatccg cctgcatatt
    12241 ttgcccgacg tttaccccgc aggagacgaa gtgctgacca tctggatggc aactggtcgc
    12301 cgcgttccac ccgctgcatt acccgttaac gtcggtgtgg tggtcaataa tgttcaaacc
    12361 gtacttaata ttgccagagc cgtcgagcaa cagtatccag tgacgcgccg cacactcact
    12421 attaatggtg cagtggcgaa accaataacg gtaaccgtcc ccatcggcat gtctttacgt
    12481 gaagtcctgg cgctggcggg cggcgcaaca gtagatgctc ctggttttat caatggcggg
    12541 ccgatgatgg gaaatttgat cacatcgctc gacacccccg tcagcaaaac cactggcggc
    12601 ttgctggttt tacccaactc tcattcactg atccaacgtc gaatgcagaa tgaccgcagc
    12661 gtacttaccg tcgcgaaaac tgtttgtgag caatgccgct tgtgtaccga tctctgccca
    12721 cgtcatttag tcggtcacga actggcaccc catttattag tacgtgcggt taactaccaa
    12781 cagctcgcta cgccacaact gttactgacg gcgctcacct gttcggagtg caacgtctgc
    12841 gccagcgtcg cctgtccggt aggaatttca ccaatgcgca taaaccgcct gcttaagcag
    12901 gaattacgcg cacaaaatct ccgctacgaa ggtgccttaa atcctgctga tcccatggca
    12961 aattatcgac tgattcccgt aaaacggctg gtaaccagac tgggcctaat cccgtggtat
    13021 caggatgcgc cattgtctga acaggtgcct cagcccgaaa aagtgacgtt attgttacgc
    13081 cagcacatcg gtgccagcgc aattgcgtgt gtacaaaaag gcgatcgcgt cgttcatggg
    13141 caatgtgtag gtcaaattcc acacggcaca ttaggtgccc ccattcatgc cagtattgat
    13201 ggcatggtca gcgatgtgac agaaaacgcg attacgcttg tgagaggtta acgatgtcga
    13261 aagcggtagg tattttagaa cttagcagta tcgcgaaagg gatggaaacc ggcgacgcca
    13321 tgctgaaaag cgcaaacgtt gagttactgg tcagcaaaac gctatgcccc ggaaaatttc
    13381 tgctgatgct cggtggcgat gtgggtgcgg tgcaacaggc catcaacagc ggtgcttccc
    13441 ttgctggtga aatgctggtc gacagtcttg tcctgccaaa tattcatccc agtctccttt
    13501 ctgcaatcag cggactgaac catgttgaac agcaccgggc tgtcggcgta gtcgaaacct
    13561 ggagtgtcgc cgcctgcatt gacgctgccg attgtgctgt caaagctgcg aatgtcattc
    13621 tcgttcgcgt acatatggct tttggcattg gtggtaaatg ctacatggtg gttgctggcg
    13681 atatttcaga tgttaataac gcggtctctg tcgccagcga gcgtgcgggg gaaaaaggtt
    13741 tgttggtcta tcggtcagtg attgcccgtc cacatgaagc catgtggcgt cagatggtgg
    13801 agggataatg acaactcagc acacaactga acggatgatt caggaatatg tccctggtaa
    13861 gcaggtcacg ctggcacatg tcatcgccaa tcctgggaag gatctgttta agaaattggg
    13921 tttaccagac accgtttctg ctattggcat tctgacgatt acccccagcg aagcatctat
    13981 catcgcatgt gatatcgcca caaaatcagg cgctgtagag atcggttttc ttgatcgttt
    14041 taccggtgcc gtagtgctga ctggtgatgt ttctgctatt gaatatgcac ttagccaggt
    14101 tacccgtacg ctgggtgaaa tgatgcgctt taccgcctgc cccattacgc ggacctgacg
    14161 ttatgcaacg tatgatgctt atcggcccca gccagtgcgg aaaaacgtca ctcacgcaat
    14221 gtctgcgtgg cgaaaatatc cattatcaga aaacgcaggc aatcgtctgg tccccgacca
    14281 ccattgatac accaggcgaa tacctcgaaa accgcagcct ctacagtgca ttgttggtca
    14341 gtgcctgcga agcagacatt atcgcgctgg tacttaatgc ggacgcaccg tggtcacctt
    14401 tttcgccagg ctttacctcg tcaatgaatc gcccggtcat tggagtagta accaaagcag
    14461 atttagccag catggaacaa atatctttgg tgaaatgttg gttacgggaa gctggagcgc
    14521 acaacgtgtt agtaaccagt gcggttaata acaatggagt tacagaactt tttgccttgc
    14581 tgcatacaga agaaggctgc cgctaatttc gataggttct cagatgagaa tgatgacggt
    14641 gatacacaga taagaatcaa tgagcaatga gggttgccgg gcaaccctca ttgaataaaa
    14701 caggaattac ttcgcttcgc cgtttttcat ttcgcccata gctttcagct ttttggagat
    14761 atcgcggcgt tctttggaga gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc
    14821 atagtcacct ttcatgctgg cgatgatgcc ctggattgct tcaacgctca tccctggctt
    14881 gatgtaatcg ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac ggatcttttt
    14941 ctcaacgtcc tgaatttcac gttgcagttt gttcttacga cggaacagac gaacaaattc
    15001 cagtacgtcc tggaatgaag gcttggtagt ttccattttt atacccctga taatgtgaga
    15061 gtcggattcg tttaagcaac cgctattcgt tagggccaac attactcata gctgccgcag
    15121 atgacaatgc ttttatccct tttcactatc atacccttta tccttgctga atcgaagcag
    15181 cagcaagatg attctgaagt tcaggaatta tttgcgcaag gcccggcaaa tcagattcga
    15241 tagcaactca agctgatgtg ccgtttccat gttcagccac acataaccat agattggtgt
    15301 ttccacaacc ttcaggtcat ggtggttatc gagcaactga cgcagctctt ccacagcatc
    15361 gttcaatttt tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac
    15421 aaggctcagc agtatttgct gcatcatgtg ctgggtatca cgaagttttt gcgcgttcaa
    15481 taacacgaaa tggctggggc gcgtggccca gtatgcattg atttgcaact ccagcataca
    15541 aaccagattg cgattaatgg tctggatacc ttcatatatc gattttggaa tacgggtttc
    15601 tttgctggcg ggcgcaatca gcccacgcat tttcacggca tcggtcagga gtttttgtag
    15661 atggctttcc agacgtgggc gttcgagtaa gttcggtgag aatgcagatt gatagacccg
    15721 attatactcg gtcagacttt tcgccagttg aatgcgccag tggatgaacg cccgttgtgg
    15781 ccagataccg gtaaacaaca ttgccagtaa agagccgagg atcacatcgc cgcttcgcca
    15841 taacgccgta tcaatttcac ctgtcgggga acccacaaca attgccagcg tcaccccaat
    15901 caataaacct tgatatggtt tcttgcccag cgccagccaa ccgcaaagga acatggccgc
    15961 cgcgcaccag actaacatca gcggtaacga gattaactcc agttgcagag cgataagacc
    16021 taaaatcgac cccaacaccg taccgccaat acgttcgaag gcacgaggga caacgttccc
    16081 ccagaacgag attggcccca taatcaccac catggtgacc agcggccagg tgctttccgg
    16141 gatagtaaac aggcggataa tgagaaaagt gagcaggaat gccagcgcga cccgagtacc
    16201 atgcacaatg cggtaatggc ggtatacccg gatttcaaac gggcttaatg acttatcggc
    16261 acgcacgcag aaactccgta atgaagatta aaagcgctca taactcatct gtcggccaga
    16321 aggcgcgcct ctgaattatc cgacggacaa accgggaaca gtgtaatttc ccggcaggag
    16381 cgaaaaaatt gaaaggcgca tcttactctt ttcgctttca aaaaaagagt ggtcttcgcg
    16441 tcaacacacc gccctgagat gaattagtga tgttttggct gcaccgcaac atacatttcg
    16501 atatcccagt acccatcttc cgcgccattg ttcaagtaaa cctcgaagca gggctttggt
    16561 aacatttcat aagcactgtc ctgcaagagg ctattaaaga actgatacca gggtttagca
    16621 aaatcatcac cgactacacg agccaccgcc accgcatact gaccacctga aatttctgtc
    16681 agaatgacgc cctcactgtt ttcggggagc gtaaagttat tcggtaccgt cacgacggtg
    16741 tcgcagcgta atttttcggc gggtgtttca tctggattgt cgtaatagac agcaacccac
    16801 tccttcggca caatattttt gctatctatc cacatcatca actgctcaaa acctttcttt
    16861 accgtctgct cccacgggcc aacaagatgg aaacctgcaa cggtacgttt ctcttcctgc
    16921 ttaatctcgt agttcatgac gcctccatta acactgtttt tatatacagt atagttgcaa
    16981 tattaaaacc acaaggaatg agtgttgatt atgcgagcag actcgcactc ctgccagtct
    17041 gctgcaaaag aaaggtcagg ccttatggtg gaaataatca ctcaggcggg aaaacatgcc
    17101 gccttccccg acagattcca gggtaaccag cggccagtgc gccacctgtt tatcacggtc
    17161 gtaaagttca atttccccta cccgctgatg ggcgctaatt ggcgcggtga gctctttacc
    17221 atcaagggta tatttggctt tgatatgtgg aatttcggct ttcggtagca ccatccagaa
    17281 ctcttgttcc gttcccaggt cgatattttc tttatcgcca taccagatgc gttccgttcc
    17341 gacctttttc ccacggtgca aaatttgcac cgtagtaaag ttttgttgcc cccaacgcag
    17401 taattttctt gcctcttcct cacgaccttt tgcactgtca gcccccatta ccactgcaat
    17461 gagacgacgc tgcccatcta cagccgaagc aatgagatta aacccggcac cagaagtatg
    17521 acccgttttc aggccgtcaa cattcatggt tttatcccac aacaacccgt tacggttttg
    17581 ctgggtgata ccgttccagg tgaggctttt ctcactgtac atatgataaa actcgggctc
    17641 gccgtggatg atagcgcgag aaagcacagc taaatcataa gccgagctat gctggccagg
    17701 tgcatccaga ccatgcactg tttcaaaatg cgtatccttg agatgcagct tctcggcata
    17761 gttgttcatc attgcaacaa actgccgttg cccaccggca atatagtcag ccagtgcaac
    17821 acaagcatca tttccggaat ccacaattaa accacggctt aaatcacgta ccgatacgcg
    17881 atcgccctct ttcaaaaaca tcagtgaaga accgacaaac actggattat ctttcgccca
    17941 cgcatcgcgc cctacggtga caatatcgtc tggcgtaatt cgatgactat cgatagcgcg
    18001 atccacgaca taacccgtca tcagctttgt caggctggcg ggattgcgct gttgatgctc
    18061 attacccgcg gtgaggatct gtccggtggt gtaatccatc aatacccagg acccggcatg
    18121 aatctctgga ggctgaggtg aaaaaggaat gttttccgcc gcaaaaccag acgataagtt
    18181 aaaaacgaac aaagaagcag caataataag acggcgtttc aacagcaaac cctcaggagt
    18241 ttcaaatagc tgttcttttt acggaaatac ttatgaactg gctggaataa agtgcaagaa
    18301 aatgtgacta acctctcatt tttatctgac atgatctgtt gccactcgct gccaaattgt
    18361 ggcgctaaag ctgattagca cggtgatatt tgatactctg gcagacagca gagataacgg
    18421 atttaaccta atgatgaatg acggtaagca acaatctacc tttttgtttc acgattacga
    18481 aacctttggc acgcaccccg cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga
    18541 tagcaaattc aatgtcatcg gcgaacccga agtcttttac tgcaagcccg cggatgacta
    18601 tttaccccag cctggagcag tattaattac cggtattacc ccgcaggagg cacgggcgaa
    18661 aggagaaaac gaagccgcgt ttgccgcccg tattcactcg ctttttaccg taccgaagac
    18721 ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca acgtttttta
    18781 tcgtaatttc tacgatcctt acgcctggag ctggcagcat gataactcgc gctgggattt
    18841 actggatgtt atgcgtgcct gttatgccct gcgcccggaa ggaataaact ggcctgaaaa
    18901 tgatgacggt ctaccgagct ttcgccttga gcatttaacc aaagcgaatg gtattgaaca
    18961 tagcaacgcc cacgatgcga tggctgatgt gtacgccact attgcgatgg cgaaactggt
    19021 aaaaacgcgt cagccacgcc tgtttgatta tctctttacc catcgtaata aacacaaact
    19081 gatggcgttg attgatgttc cgcagatgaa acccttggtt cacgtttccg gaatgtttgg
    19141 ggcatggcgc ggcaatacca gctgggtggc accgctggcg tggcatcctg aaaatcgcaa
    19201 tgccgtaatt atggtggatt tggcaggaga catttcgcca ttactggagc tggatagcga
    19261 cacattgcgc gagcgtttat ataccgcaaa agccgatctt ggcgataacg ccgccgttcc
    19321 ggttaagctg gtgcatatca ataaatgtcc ggtactggcc caggcgaata cgctacgccc
    19381 ggaagatgcc gaccgactgg gaattaatcg tcagcattgc ctcgataacc taaaaattct
    19441 gcgtgaaaat ccgcaagtgc gtgaaaaagt ggtggcgata ttcgcggaag ccgaaccgtt
    19501 tacgccttca gataacgtgg atgcacagct ttataatggc tttttcagtg acgccgatcg
    19561 tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac tggatatcac
    19621 ttttgttgat aaacgaattg aaaaactgtt gttcaattat cgagcgcgca acttcccggg
    19681 gacgctggat tatgccgagc agcaacgctg gctggagcac cgccgccagg tcttcacgcc
    19741 agagtttttg cagggctacg ctgatgaatt gcagatgctg gcacaacagt atgctgatga
    19801 caaagagaaa gtggcgctgt taaaagcgct ttggcagtac gcggaagaga tcgtct
//
LOCUS       DAZGAP010000099        19763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGAP010000099 DAZGAP010000000
VERSION     DAZGAP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.099"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..303)
                     /gene="ybfA"
                     /locus_tag="ACHLAM_003016"
     CDS             complement(97..303)
                     /gene="ybfA"
                     /locus_tag="ACHLAM_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO7854510.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            616..705
                     /gene="kdpF"
                     /locus_tag="ACHLAM_003017"
     CDS             616..705
                     /gene="kdpF"
                     /locus_tag="ACHLAM_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO7854511.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            705..2378
                     /gene="kdpA"
                     /locus_tag="ACHLAM_003018"
     CDS             705..2378
                     /gene="kdpA"
                     /locus_tag="ACHLAM_003018"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO7854512.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFIICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLALMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2401..4449
                     /gene="kdpB"
                     /locus_tag="ACHLAM_003019"
     CDS             2401..4449
                     /gene="kdpB"
                     /locus_tag="ACHLAM_003019"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO7854513.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPVDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4458..5030
                     /gene="kdpC"
                     /locus_tag="ACHLAM_003020"
     CDS             4458..5030
                     /gene="kdpC"
                     /locus_tag="ACHLAM_003020"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO7854514.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5023..7707
                     /gene="kdpD"
                     /locus_tag="ACHLAM_003021"
     CDS             5023..7707
                     /gene="kdpD"
                     /locus_tag="ACHLAM_003021"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO7854515.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7704..8381
                     /gene="kdpE"
                     /locus_tag="ACHLAM_003022"
     CDS             7704..8381
                     /gene="kdpE"
                     /locus_tag="ACHLAM_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO7854516.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8615..8719
                     /locus_tag="ACHLAM_003023"
     CDS             8615..8719
                     /locus_tag="ACHLAM_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854517.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            8977..11175
                     /gene="speF"
                     /locus_tag="ACHLAM_003024"
     CDS             8977..11175
                     /gene="speF"
                     /locus_tag="ACHLAM_003024"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO7854518.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11172..12491
                     /gene="potE"
                     /locus_tag="ACHLAM_003025"
     CDS             11172..12491
                     /gene="potE"
                     /locus_tag="ACHLAM_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO7854519.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12548..14188)
                     /gene="pgm"
                     /locus_tag="ACHLAM_003026"
     CDS             complement(12548..14188)
                     /gene="pgm"
                     /locus_tag="ACHLAM_003026"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO7854520.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14214..14759)
                     /gene="seqA"
                     /locus_tag="ACHLAM_003027"
     CDS             complement(14214..14759)
                     /gene="seqA"
                     /locus_tag="ACHLAM_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO7854521.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            14944..15708
                     /gene="ybfF"
                     /locus_tag="ACHLAM_003028"
     CDS             14944..15708
                     /gene="ybfF"
                     /locus_tag="ACHLAM_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7854522.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15848..16141
                     /gene="ybfE"
                     /locus_tag="ACHLAM_003029"
     CDS             15848..16141
                     /gene="ybfE"
                     /locus_tag="ACHLAM_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO7854523.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16281..16811
                     /gene="fldA"
                     /locus_tag="ACHLAM_003030"
     CDS             16281..16811
                     /gene="fldA"
                     /locus_tag="ACHLAM_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO7854524.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17021..17107
                     /gene="uof"
                     /locus_tag="ACHLAM_003031"
     CDS             17021..17107
                     /gene="uof"
                     /locus_tag="ACHLAM_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO7854525.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            17100..17546
                     /gene="fur"
                     /locus_tag="ACHLAM_003032"
     CDS             17100..17546
                     /gene="fur"
                     /locus_tag="ACHLAM_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO7854526.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17630..17956)
                     /gene="chiQ"
                     /locus_tag="ACHLAM_003033"
     CDS             complement(17630..17956)
                     /gene="chiQ"
                     /locus_tag="ACHLAM_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO7854527.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18006..19412)
                     /gene="chiP"
                     /locus_tag="ACHLAM_003034"
     CDS             complement(18006..19412)
                     /gene="chiP"
                     /locus_tag="ACHLAM_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO7854528.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDIYDGTAWLQALTFGYRAADVVDLRLEGSWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4540 a         5006 c         5239 g         4978 t
ORIGIN      
        1 taacggtgtc taaatattcc tctgaacatc ttcctgatac aaaaaaaacc gccagcgttt
       61 ccgctggcgg ttttcaatgt attcagaact cagcaatcag taaaaatcac cagtcgcctt
      121 ttcggcctga tccatccaca ccggtttatc gctggttttc gaccagacgc ggtgcaggta
      181 gctgtaaaag cgggcgcggt ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc
      241 tgcaaccgca taagtacggc gtaaaaagat aagccatgca ggatattctc tgtagagttc
      301 catacttttc tccccctttt gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt
      361 tatctgatga aaatagtacc gccttttgtg taattttact actcatccga ccacttattt
      421 ttgcttattg atggtttatt tacattcata ccgtaattaa gttacacaaa ggttaaatta
      481 atactaaaca ttagttaaat catggctttt gccattttta tacttttttt acaccccgcc
      541 cgcagatttt tgcgaaatct ttgcagccag aattctactc ttccggtatc acttttaggc
      601 cactggaggt gcactgtgag tgcaggcgtg atagccggcg tgttgctggt gtttttatta
      661 ctgggttatc tggtttatgc cctgatcaat gcggaggcgt tctgatggct gcgcaagggt
      721 tcttactgat tgccacgttt ttactggtgt taatggtgct ggcgcgtcct ttaggcagcg
      781 ggctggcgcg gctgattaat gacattcctc ttcccggtac gacgggcgtt gagcgcgtac
      841 tttttcgcgc gcttggcgtc tctgaccgcg agatgaactg gaagcagtat ctctcagcca
      901 ttcttggcct gaatatactg gggctggcgg tgctgttttt tatgttgctc ggtcagcact
      961 atctgccgct taatccacag cagttgccag gactgtcgtg ggatctggcg ctgaataccg
     1021 ccgtcagctt tgtcacaaat accaactggc aatcttacag cggtgaaacc acgttgagct
     1081 atttcagcca gatggcgggc ttaacggtgc aaaactttct ttctgccgcc agcgggattg
     1141 cggtgatttt tgccctcata cgtgcattta cccgccagag catgagcaca ctcgggaatg
     1201 cctgggtcga tctgctacgc atcacgttat gggtgctagt ccctgtggcg ttgttgatcg
     1261 cattgttttt tattcaacaa ggtgcgctgc aaaactttct gccctatcag gcggtgaata
     1321 ccgttgaagg agcgcaacag ctgttaccca tggggcctgt agcttctcag gaagcgatca
     1381 agatgctcgg cactaacggc ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa
     1441 acccaaccgc actgaccaac ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc
     1501 tgtgctttgc ctttggcgaa gtggcgggcg atcgccgtca ggggcgcatg ttgctgtggg
     1561 cgatgtcagt gatttttatc atctgcacag gcgtggtgat gtgggcagaa gttcagggta
     1621 atccgcatct gctggcactg ggcgcggaca gcagcatcaa tatggaaggt aaagagagtc
     1681 gtttcggcgt gctggtcagt agcctgtttg cggtcgtgac tacggcggct tcctgtggcg
     1741 cggtgattgc gatgcatgat tcgtttaccg ctcttggtgg catggtgccg atgtggctga
     1801 tgcaaattgg tgaagtggtg ttcggcggtg tcggttctgg tctttacggc atgatgctgt
     1861 ttgtcctgct ggcggtgttt attgctgggc tgatgattgg tcgtacaccg gaatatctgg
     1921 gtaaaaaaat cgacgtacgc gagatgaaac tgaccgcgct ggcgattctg gtcaccccaa
     1981 cgctggcgct gatgggcgcg gcgctggcaa tgatgaccga cgccggacgt agcgccatgc
     2041 tcaaccctgg cccgcatggt tttagcgaag tgctgtacgc cgtgtcatcc gccgctaaca
     2101 acaacggcag cgcctttgcc ggattaagcg ccaactctcc attctggaac tgtttgctgg
     2161 cgttctgcat gtttgtcggt cgcttcgggg taattatccc ggtgatggca attgccggtt
     2221 cgctggtgag taaaaagagc cagcccgcca gctccggcac gctgccaacg cacggcccgc
     2281 tgtttgttgg cctgttaatc ggcaccgtgt tgctggttgg cgcactgacc tttatccctg
     2341 ccctggcgct tggtccggtg gcggaatatc tctcctgatg atattgagtg agcactgaat
     2401 atgagtcgta aacaactggc gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa
     2461 gcggtgaaaa aattaaaccc gcaggcgcaa tggcgtaatc cagtgatgtt tatcgtctgg
     2521 atcggcagtc tgttaaccac ctttattagc attgcaatgg caagcggcgc gatgcctggc
     2581 aatgcgctgt ttagcgcggc aattagcggc tggctgtggg tcaccgtact gttcgctaat
     2641 ttcgccgagg cgctggcaga aggccgcagt aaagcgcaag ccaatagtct gaaaggggtg
     2701 aaaaaaactg cctttgcccg caagctgcgt gagccgaaat atggcgctgc ggcggacaaa
     2761 gttcccgcag atcaactgcg taaaggcgat atcgtactgg tagaagctgg cgatattatc
     2821 ccctgcgatg gtgaagtcat tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg
     2881 gaatcggctc cggtgatccg tgaatccggc ggcgattttg cctccgtcac tggcggcacg
     2941 cgtattcttt ctgactggct ggtgattgag tgcagcgtta accccggcga gacatttctg
     3001 gatcggatga tcgcgatggt ggaaggcgcg cagcgacgca aaacgccgaa cgagattgcc
     3061 ctgaccatcc tgctgattgc cctgactatc gtctttttac tggcaaccgc cacgctgtgg
     3121 ccgttttccg catggggtgg taatgcagtc agcgtaacgg tactggtggc gctgttggtc
     3181 tgtctgatcc caaccactat tggcggcctg ttgtcagcga tcggcgtcgc cgggatgagc
     3241 cggatgttgg gcgcgaatgt gattgccacc agcgggcgtg cagttgaagc ggcgggcgac
     3301 gtggacgtcc tgctgctgga taaaactggc accatcaccc tcggtaaccg tcaggcatca
     3361 gagtttatcc ctgcgcaggg cgtagaggaa aaaacgctgg ccgacgcagc acaactggct
     3421 tcgctggccg acgaaacgcc ggaaggccgc agtattgtga tcctcgccaa gcagcgcttt
     3481 aacctgcgcg agcgcgatgt gcagtcgctt cacgccactt ttgtaccgtt tactgcgcaa
     3541 agtcgaatga gcgggatcaa tatcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc
     3601 attcgccgcc atgttgaggc taacggtggt cacttccctg tcgatgttga tcaaaaagtc
     3661 gatcaggttg cgcgtcaggg ggccacgccg ctggtggtgg tggaaggttc tcgtgtgctg
     3721 ggcgttattg cgctgaaaga tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg
     3781 cgcaaaatgg gcattaaaac ggtgatgatt accggcgata accgtctgac tgccgccgcc
     3841 attgctgcgg aagcgggtgt cgatgatttt ctcgccgaag cgacaccgga ggccaagctg
     3901 gcgttaattc gtcagtatca ggcggaaggc cgtttggtgg cgatgaccgg cgacggcacc
     3961 aatgatgctc cggcgctggc gcaggcagat gtcgcggtag cgatgaactc cggtactcag
     4021 gcggcgaaag aagcgggcaa tatggtggat ctcgactcta acccgaccaa gttgatcgag
     4081 gtggtgcata ttggcaaaca gatgctgatg acccgtggct cgctgaccac cttcagcatt
     4141 gccaacgatg tggcgaaata cttcgccatt attccggcgg cgttcgcggc aacgtatccg
     4201 cagttaaatg cgctgaacat catgcgcctg cattcgcccg actccgccat tctcagtgcg
     4261 gtcattttca acgccttgat tatcgtcttt ttgattcccc tggcgttaaa aggcgtgagt
     4321 tataaaccgc ttaccgcttc tgccatgttg cgccgtaact tatggattta cggtctgggt
     4381 gggctgctgg tgccgtttat tggtatcaaa gtcattgatt tactgctgac cgtttgcggt
     4441 ctggtgtgag gtttaccatg cgtggattac gtccggcatt atcaacattt ctctttttgt
     4501 tactgattac tggcggtgtt tacccgctgc tgaccaccgc gctggggcaa tggtggtttc
     4561 cctggcaggc caacggttcg ttgattcgcg aaggtgatac ggtgcgcggc tcggcattga
     4621 tcgggcagaa ttttactggc aacggctatt ttcatggtcg cccgtcggca acggcagaaa
     4681 tgccctataa tccacaggct tctggcggga gcaatctggc ggttagtaac ccggagctgg
     4741 ataaacaaat cgctgcacgc gttgccgcat tacgggccgc taacccggat gccagcgcga
     4801 acgttccggt tgaactggtg acggcgtcgg caagcgggct ggacaataac atcaccccgc
     4861 aagcggcggc ctggcagatc ccacgcgtgg cgaaagcgcg taatctcagc gttgaacagc
     4921 tcacgcagct gatcgcaaaa tatagccaac aaccgctggt gaaatatatc ggccagccgg
     4981 tcgtcaacat tgtcgaactc aatctggcgc tggataagct tgatgaataa cgaaccctta
     5041 cgtcccgacc ccgatcgtct gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa
     5101 gttttcttcg gtgcctgcgc aggcgtcggc aagacctggg caatgctggc agaagcccag
     5161 cgactgcggg cgcaagggct ggatattgtg gttggcgtgg tagaaaccca cgggcggaaa
     5221 gataccgccg caatgctgga agggctggct gttctgccgt taaaacgcca ggcgtaccgt
     5281 gggcggcaca tcagcgagtt tgatctcgat gccgccctcg cccgccgtcc ggcactgatc
     5341 ttaatggacg aactggcgca cagtaatgcg ccaggttctc gtcatcccaa acgctggcag
     5401 gacatcgaag agctgctgga agctggcatt gatgttttca ctaccgtcaa cgttcaacat
     5461 ctggaaagcc tgaatgatgt ggtcagcggc gtcaccggaa ttcaggttcg ggaaaccgtg
     5521 cccgatcctt tttttgatgc cgccgacgac gtagtgctgg tggacttgcc cccggacgat
     5581 ctgcgccagc gactgaaaga aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt
     5641 gaacattttt tccgcaaagg caatctgatc gccctgcgcg aactggcact gcgccgtact
     5701 gccgatcgcg tagatgagca aatgcgcgcc tggcgggggc atcctggcga agagaaagtc
     5761 tggcacacgc gcgacgcgat ccttttatgc atcggccata acactggtag tgagaaactg
     5821 gtccgcgcgg cggcgcggct ggcgtcacgg ctgggtagcg tctggcacgc ggtgtatgtt
     5881 gaaacccctg ccctgcatcg cttaccggag aaaaaacgtc gggcaattct cagcgcctta
     5941 cgtctggcgc aggagctggg cgcggagacg gcaacacttt ctgatccagc ggaagagaaa
     6001 gcggtagtgc gttacgcccg tgagcataat ctcggcaaga ttattctcgg tcgcccagcc
     6061 tcgcgccgct ggtggcgtcg ggaaacgttt gctgaccgac tggcgcgcat cgcccccgat
     6121 ctcgatcagg tgctggtcgc gcttgatgaa ccacccgccc gcacgattaa caacgcgccg
     6181 gatagccgct cttttaaaga caagtggcgg gtacagattc agggatgcgt ggttgccgcc
     6241 gcgttatgcg ccgttatcac cttaattgcc atgcagtggc tgatggcgtt tgatgccgcc
     6301 aacctggtga tgctgtatct acttggcgtg gtggtggtgg cgctatttta tggacgctgg
     6361 ccttcggtgg ttgctaccgt cattaatgtg gtgagtttcg atctcttttt tatcgcccca
     6421 cgcggcacgc tcgccgtctc tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc
     6481 gtcgggctgg tgatcgggaa ccttactgct ggcgtgcgtt atcaggcgcg ggtagcccgt
     6541 taccgcgaac aacgcacacg gcacttatat gaaatgtcca aagctctggc tgtgggccgc
     6601 agttcgcagg atatcgccgc caccagcgaa cagtttattg cctccacgtt tcatgcccgt
     6661 agccaggtgt tgctgcctga tgacaacggt aaattgcagc cgttaacaca tccgcaagga
     6721 atgacgccgt gggacgatgc catcgcgcag tggagttttg ataaaggtct gcctgcgggc
     6781 gcgggcaccg acacgttacc cggtgtaccg taccagattt tgccgctaaa aagcggcgag
     6841 aaaacctacg gactggtggt ggtggaaccg gggaatctgc gccagttgat gatcccggaa
     6901 cagcagcgcc tgctggagac gtttacgctg ttagtcgcca atgcgcttga gcggctgacg
     6961 ctgaccgcca gcgaagaaca ggcgcggatg gcaagcgaac gtgaacagat ccgcaacgct
     7021 ctgctggcgg cgctttcgca tgatttacgc acgccgctca cggtgctgtt cggtcaggca
     7081 gaaatcttaa cgctcgatct ggcaagcgaa ggatcgcccc acgcccgcca ggccagcgag
     7141 attcgtcagc atgtgctgaa cacgacccga ctggtgaata atctgctcga tatggcgcga
     7201 attcagtccg gcggctttaa tttgaagaaa gagtggttaa cgctggaaga agtggtcggc
     7261 agcgcgctgc aaatgctgga accgggttta tcgtcgccta tcaatctttc tctgccagaa
     7321 ccgctgacct taatccacgt tgacgggccg ctctttgaac gggtgctgat taatctgctg
     7381 gagaacgcgg tgaaatacgc gggtgcgcag gccgaaattg gtatcgatgc ccacgttgag
     7441 ggcgagaagc tacaactgga tgtctgggat aacggccccg gtcttccgcc aggccaggag
     7501 cagacgatat ttgataagtt tgctcgcggg aataaagagt cggcggtacc gggtgtaggg
     7561 cttgggctgg caatttgtcg ggcgattgtg gatgtacacg gcggcaccat taccgcgttc
     7621 aaccgaccgg aaggtggtgc ctgttttcgt gttacacttc cccagcaaac cgctcctgaa
     7681 cttgaagaat ttcatgagga tatgtgacaa acgttctgat tgttgaagat gaacaggcta
     7741 ttcgtcgctt tctgcgcacg gcgctggaag gcgacggtat gcgcgtcttt gaggccgaaa
     7801 cgctgcaacg cggcttgctg gaagcggcta cccgtaagcc agatttgatt attctcgatc
     7861 tcggcctgcc cgatggtgat gggattgagt ttatccgcga cctgcgccag tggagcgcgg
     7921 tgccggtgat tgtgctttcc gcacgtagcg aagagagcga caaaatcgcc gcgctggatg
     7981 ccggagcgga tgattatctg agtaagccgt ttggcattgg cgaattgcag gcccgtctgc
     8041 gcgtcgcttt acgtcgccac tccgccaccg ccgcgcccga tccgctggtg aaattttccg
     8101 atgttaccgt cgatttagca gctcgtgtga tccatcgggg cgaggaagag gtgcatctca
     8161 ccccgattga gttccgcctg ctggcagtgt tgcttaataa tgcgggaaaa gtgctcaccc
     8221 agcgccagct ccttaaccag gtgtgggggc caaacgcggt cgaacacagt cattatttgc
     8281 gtatttatat ggggcatctg cgacaaaaac tggaacagga cccggcccgg cctcgccatt
     8341 tcattactga aaccggtatt gggtatcggt ttatgctttg aatattaatt tcaatacatc
     8401 ctgcctttta ttaattaaag ccgtaataat aaatacggct ttttatctta aacaacacac
     8461 aaaaataaca attcaatatt ttatattact gagtaaaaag ctcatcatta aataaattaa
     8521 gattgatcat tttttattga tcaccttcac agttcaaccg tatttcctgg atagaatacc
     8581 ttcaccttca ttcacatcag gaaaggtaaa ttaaatggaa aataacagcc gcactatgcc
     8641 ccatataagg cggacaactc atattatgaa gtttgctcat cgcaatagct tcgactttca
     8701 cttcttcaat gcccgttagt ctaccgacta agggcacttc agcgtacagg tcttcctgac
     8761 tctctgtatt acaggcatat gcctgtattc cgcagtgctc attctctgtt aacccttgta
     8821 ttatcagcca ccggactgat aagggtttct gcattctgaa aaaagcaaac ttctgattaa
     8881 tttatcagcg gggtaacttt gctttttttc cggcacgatc gatttctcat tcgagaaatt
     8941 gaggacctgc tattacccaa aataaagaga tggaaaatgt caaaattaaa aattgcggtt
     9001 agtgattctt gcccggactg ttttaccacg cagcgagaat gtatctatat taatgaaaac
     9061 agtaatatcg atgtggcggc aatagtttta tcgctcaacg atgttacacg cggaaaactc
     9121 gatgaaatcg atgccacggg ttatggcatc ccggtattta ttgctactga aaatcaagaa
     9181 cgtgtacccg cagagtattt gccccgtatt tcgggtgtct ttgagaattg cgaatcgcga
     9241 cgagaatttt atggtcgtca gttagaaacc gcagccagcc attatgaaac gcaactgcgc
     9301 ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gtaacagcgc gtttgattgc
     9361 cccggtcatc agggcggcga atttttccgt cgccatcccg cggggaatca gtttgtggaa
     9421 tactttggtg aggcgctgtt ccgtgctgac ttgtgcaacg ccgacgtagc gatgggcgat
     9481 ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa agtgtttaat
     9541 gccgataaaa catacttcgt tttaaatggc acttcatctt ctaacaaagt ggttttaaac
     9601 gccctgctga caccgggtga tctggtgctg tttgaccgca ataaccacaa atctaaccac
     9661 cacggagcgt tgctacaggc tggcgcgaca ccggtttatc tggaaaccgc acgtaacccg
     9721 tatggtttta tcggtggtat tgacgctcac tgttttgaag aaagttacct gcgtgaactg
     9781 atcgcggaat tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct cgccgttatt
     9841 cagctaggca cttacgacgg tacgatttat aacgcccgtc aggtggtgga taaaattggt
     9901 catctgtgtg actacatcct ctttgactcc gcatgggtcg gctatgagca gtttatcccg
     9961 atgatggcgg actgttcacc actgttgctg gatcttaatg agaacgatcc gggtattctg
    10021 gttacgcaat ctgtgcataa acagcaggct ggtttttccc agacttcaca aatccataaa
    10081 aaagacagcc atatcaaagg gcaacagcgt tatgtaccgc acaaacgcat gaacaacgcc
    10141 tttatgatgc atgcctccac cagcccgttc tatccgctgt ttgccgcact ggatatcaac
    10201 gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt ggtaaatggc
    10261 attaatgccc gcaaactaat cctcgataac tgtcagcata ttcgtccatt cgtacctgaa
    10321 ctggtggatg gtaaaccctg gcagtcgtat gaaacggcgc aaattgcggt tgatctgcgc
    10381 ttcttccagt ttgtaccagg tgagcactgg cattcttttg aaggctatgc agagaatcaa
    10441 tactttgtcg atccgtgcaa actgttgctg acaaccccag gtattgatgc acgtaacggc
    10501 gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg cgaaaatggc
    10561 gtagtgccgg aaaaatgcga tcttaactcc atcctctttc tgctgactcc ggcagaagat
    10621 atggccaaac ttcagcaact tgttgctctg ctggtacgct tcgaaaaact gcttgagtcc
    10681 gacgcgccat tatcagaagt actgccttcc atctacaaac agcatgaaga gcgctacacc
    10741 ggttataccc tgcgtcagtt gtgtcaggaa atgcatgatt tgtatgcccg tcacaacgtg
    10801 aaacaactgc aaaaagagat gttccgtaag gaacacttcc cacgcgtaag catgaatcca
    10861 caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct gccggatgca
    10921 gaaggccgta tcgctgccga aggtgcgctt ccttatcctc cgggtgtgct gtgtgttgtt
    10981 ccgggtgaaa tctggggtgg tgctgttctg cgttacttca gcgctctgga agaagggatc
    11041 aacctgctgc caggttttgc accagagctg cagggtgtct atatcgaaga acatgatggt
    11101 cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac cctgttgaaa
    11161 ggggaaaaat tatgagtcag gctaaatcga ataagatggg cgtcgttcag ttaaccatac
    11221 tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag cttgccgaag
    11281 ttgggacaat ctcaattatc tcctggctgg tgacagccgt aggctcaatg gcactggcat
    11341 gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg cggtatgggc ggttatgcgg
    11401 agtatgcttt cggtaaatcc gggaacttta tggcgaacta cacctacggt gtctcgctgc
    11461 tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa ctgctcggcg
    11521 cgagtttgtc gccagtgcag attggtcttg cgaccatcgg agtgctgtgg atttgtaccg
    11581 tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattact gtgtgggggg
    11641 tcattattcc ggtcgtcggt ctgtgcatta tcggctggtt ctggtttagc ccgacgctat
    11701 acgctaactc atggaacccg caccatgtgc cgttctttag cgctgtaggt tcttccatcg
    11761 ccatgacgtt gtgggctttc ctcggtctgg agtcagcctg tgccaacgcc gaagtagttg
    11821 aaaacccgga acgtaacgtg ccgattgcag ttcttggcgg tacgttaggt gcggcggtga
    11881 tttatatcgt ctccaccaac gtgattgccg ggattgtgcc aaatatggag ctggcaaatt
    11941 caacggcacc atttggtctg gccttcgcgc agatgttcac gccggaagtg ggtaaagtca
    12001 ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag ttcaccattg
    12061 cccaggtgtt taaatcttct gccgatgaag ggtacttccc gaaaattttc tcccgtgtga
    12121 gcaaagtcga tgcaccagta cagggaatgc tgaccattgt catcatccag agcggattat
    12181 cactgatgac cattagcccg tcgctgaaca gtcagttcaa cgtgctggtt aacctggccg
    12241 tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt attcagaagg
    12301 tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt gttggcgcga
    12361 tgtacagctt ttatgcgctg tactcatccg gggaagaagc catgctatac ggttccatcg
    12421 tgaccttcct cggttggaca ctgtatggtc tggtctcacc acgctttgaa ctgaaaaata
    12481 aacacggtta attaagcaaa gcaaaaaagg gcgatcttcc gaccgccctt tttcttattt
    12541 gatatgttta cgcgtttttc agaacttcgc taacaatctc aaccgcttct ttctcaatct
    12601 gcttgcgatg ttcttcacca aggaagcttt cgcagtagat cttatatgcg tcttccgtgc
    12661 ctgacggacg cgcggcgaac cagccgttgt cagtcatcac tttcagaccg ccgatagaag
    12721 cgccattacc cggtgccgcc gtcagacgcg cggtgatcgg gtcacctgcc agggtgctgg
    12781 cgctcaccat ttccggagac agcttagaca acgccgcttt ttgtgcggaa gtcgctgctg
    12841 cctgcaaacg gttatagctc ggcgcaccaa agcgttttgc cagttcgtta tagtgttcct
    12901 gcgggttctt accggtgaca gcggtgattt ccgccgccag cagacacata atgataccgt
    12961 ctttatcggt ggaccacggc gtgccgtcga aacgcaggaa ggaagcgcct gcactctctt
    13021 cgccgccaaa gccgaagctg ccatcgaaca aaccatcaac aaaccatttg aaacctaccg
    13081 gtacttctac cagcttacgg cccaaatcat tgaccacacg gtcgatcatc gcagatgaaa
    13141 ccagcgtttt accgacagca acatctttgc cccactgcgg acgatgctgg aacaggtagt
    13201 tgatcgccac cgccaggtag tggttcggat tcatcaaacc tgccggagtg acgataccgt
    13261 gacggtcata atccgggtcg ttagcaaacg ccagatcgaa cttatcacgc agtgccagca
    13321 ggcccgccat cgcacactcg gaggagcagt ccatacggat cgcaccgtct ttatcgaggt
    13381 gcataaagcg gaaggtttga tcgacctgat cgttaacgat ggtcaggttg aggttgtaat
    13441 actcgccaat gcgcttccag tattcgatac cggaaccacc cagcggatca acgcccagcg
    13501 tcaggcccgc tttctgaatc gccgccatat caacgatatc ggccagccct tccacaaacg
    13561 gctgcaccag atcctgctct ttcacatgac cggatgccat cgcttcgtcg agggagatac
    13621 gcttcacgcc tttcaggcca tcagccagca gtgcgttggc tctgtcttcc accactttag
    13681 tgacgttggt atcagccggg ccaccatttg gcggattgta cttgatacca ccatcttccg
    13741 gcgggttatg ggacggtgta atcacgatac cgtctgccag tgggccacct tttttattgt
    13801 gaaccagaat ggcattggaa atggcaggtg tcggagtgaa gccattgttt tcctgcacaa
    13861 tgacatcaac gccgttcgct gccagcactt ccagtacgga aatgaacgca ggctcggaca
    13921 gggcgtgagt atctttaccc acatagcaag ggccagtgat gccgtttttc gcacgttctt
    13981 cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt aaagctgtgg cgcgctgcac
    14041 tgccacggtg accggaagta ccgaatttca ccgcgtgctc cgcattccct gcttctggtt
    14101 tcagtacata atattgcgcc gtcagttggg cgacgttaat caaatcactc tgttgtgcag
    14161 gttggcctgc acgattgtgg attgccattg ctttgtcctt tgtctgcaac gttttagata
    14221 gttccgcaaa ccttctcaat caattccgcc gggaattgca tcgactgcat gatgtgttcg
    14281 atcatgctgc atttacggcc ggtgttggtg ttggtgatca cccaatacgg cgtaccaggc
    14341 acatgtttcg gcttggtctg attaccattt ttcagcagcg tttgttcatc tgccgcaaag
    14401 taaacgcgtg tacgaccgtg caacgattcc gttgcttcgg caaacgcctg ggcgtcaaga
    14461 gaatatagtg tagacaacag cagcataaag cgattgaccg ctcgcttttg ctctgcgtat
    14521 tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt ctttaatcgt tttgaccggc
    14581 ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt tcgtcaccgg agcagcaggc
    14641 tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt cggatgcgct ctcgccgata
    14701 tgcttagtgt ggctggcaat atagctgtag agttcatcat caacttcaat cgttttcatc
    14761 ttaatccagt gcagtgtctt agctgaatac aagtcgccag gagtatatga ataaatcccg
    14821 gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg aataaacctt atttgttgca
    14881 gccctgtggc acaatggtca acataatacc ctaacccgac aacgcgaaaa aaagaacttt
    14941 gccatgaaat tgaatatccg cgcgcaaact gcacaaaacc agcacaataa ttctcccatc
    15001 gttcttgtcc atggtctgtt tggcagcctc gacaaccttg gcgtactggc tcgcgatctg
    15061 gtaaacgatc acaatatcat ccaggttgat atgcgtaacc acggtctttc accgagagat
    15121 ccggtaatga attacccggc gatggcgcag gatcttgttg ataccctgga tgcacagcag
    15181 atcgacaaag caacatttat cggtcactcc atgggcggta aagcggtaat ggctcttact
    15241 gcactagccc ccgatcgcat tgacaagctg gtggcgattg atattgcgcc ggtcgattat
    15301 cacgttcgcc gtcatgatga gattttcgcg gccatcaacg cagtcagtga atcagacgca
    15361 caaacacgcc agcaagcagc ggcaataatg cgccagcatc ttaatgaaga aggggtgatt
    15421 cagtttctgt tgaaatcttt tgttgacggg gagtggcgct ttaacgtgcc agtattgtgg
    15481 gatcagtatc cgcatattgt gggttgggag aaaatcccgg catgggatca tcctaccctg
    15541 tttatccctg gcggcaattc tccgtatgtt agcgagcagt accgtgatga tttactggca
    15601 caatttccac aggctcgggc gcatgtgatt gcaggcgcgg gtcactgggt ccatgctgaa
    15661 aaaccggatg cggtattacg cgctatccgt cgctatctca atgattaacg caactgatta
    15721 aaaacccagc gtccgcgtcg ctttccgcgc gtggacgttg gcgtgactca tcgcgctgat
    15781 gtattatggc gcgctatcca tccgggcagg cgcctggctg attgtttccc ccgaagtcac
    15841 caagatcatg gccaaagaac aaacggaccg tacgacatta gatctgttcg cgcacgagcg
    15901 tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat gagcagctgc gtattaataa
    15961 acgcaaccaa ctaaaacgcg acaaagtacg tggccttaag cgtgtcgaac tgaagctgaa
    16021 cgcggaagct gtcgaggcac tgaacgagct ggcggaatcg cgcaatatga gccgtagcga
    16081 gctgatcgaa gagatgttaa tgcagcaact ggcggcgctg cgtagtcagg gcatcgttta
    16141 aatttccact ttcatgtagc acagtgtgca gtcctgctcg tttgctgatt ccgcaagact
    16201 gcctgttctg ctatgattgc ctttatccgt gggcaatttt ccacccccat ttcaataagt
    16261 ttcaagaggt tatttcactc atggctatca ctggcatctt tttcggcagc gacaccggta
    16321 ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg taaagacgtt gccgatgtcc
    16381 atgacattgc aaaaagcagc aaagaagatc tggaagcata tgacattctg ctgctgggca
    16441 tcccaacctg gtattacggc gaagcgcagt gtgactggga tgacttcttc ccgactctcg
    16501 aagagattga tttcaatggt aaactggttg cgctgtttgg ttgtggcgac caggaagatt
    16561 acgcagaata cttctgcgat gcattaggca ccatccgcga catcattgaa ccgcgcggcg
    16621 caactatcgt tggtcactgg ccaaccgcgg gctaccactt cgaagcgtca aaaggtctgg
    16681 ctgatgatga ccactttgtc ggtctggcta tcgacgaaga tcgtcagccg gaactgactg
    16741 ctgaacgtgt agaaaaatgg gttaaacaaa tttctgaaga gctgcatctc gacgaaattc
    16801 tcaatgcctg atgtgatgcg gcgtagactc atgtctacgc cgtattaata gataatgcca
    16861 atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt acctgtacaa tgtcccggtg
    16921 ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg tttttattaa caatatttgc
    16981 cagggacttg tggttttcat ttaggcgtgg caattctata atgatacgca ttatctcaag
    17041 agcaaattct gtcacttctc ctaatgaagt gaaccgctta gtaacaggac agattccgca
    17101 tgactgataa caataccgcc ctaaagaaag ctggcctgaa agtaacgctt cctcgtttaa
    17161 aaatcctgga agttcttcag gagccggaca accatcacgt cagtgcggaa gatttataca
    17221 aacgtctgat cgatatgggt gaagaaattg gtctggctac ggtatatcgc gtactgaacc
    17281 agtttgacga tgctggtatc gtcacccgcc acaattttga aggcggtaaa tccgtatttg
    17341 aactgacaca gcaacatcac cacgatcacc tgatctgcct cgactgcggc aaggttatcg
    17401 aatttagtga tgattccatc gaagcgcgtc agcgtgaaat cgccgcaaaa catggcattc
    17461 gcctgactaa ccacagtctc tatctttacg gtcactgtgc cgaaggcgat tgccgcgaag
    17521 atgagcacgc gcacgaaggc aaataagcca gcctgaacga gaaaagccaa cctgcaggtt
    17581 ggctttttta tacaagggaa ataagaaaag cgagagttac agctctcact tatttgttat
    17641 tactgcgaat ttcctgccag accttatcac aatcggcttt caccgcctga tcatttccgg
    17701 tttgcgttgc ctgaatacac tgctgataat ccagtacgcg gacactttcc tgctcggcaa
    17761 actgctgatg ttgtttctct ttcttcagca cgtttaacac gctctggcag gcttcaattt
    17821 tttccggcga accttgcgcg gtgttgatac aggcgctgta agcctctttc agacgactgt
    17881 cttcctgcgg tgcggttgat tgcgcacaag ctaccagccc cgatgccatc atggcgatga
    17941 gaatcagttt tttcatagca aattcctcaa atgtgcggca ggcacaaaac ctgccgcgtc
    18001 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcgcg ctcatcctgg
    18061 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18121 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta caccgcatcc
    18181 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18241 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18301 ggaagattcc agtttttcag gtcatacatc gcaccgaaga agaccgcttt ttcgccgttg
    18361 gcgttgaagt cagaacggtt atcccaccag atatccaggc gaccgttcga ggaagcgtag
    18421 gttggagtca tacgttgcag gaagtatccc tgctgaccgt cggctttaac ccagctgcct
    18481 tccagacgca aatccactac atcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18541 gcagtgccgt cgtaaatgtc gttgacgctg cgatcgtcaa ctttatcgcg cgtaccgtag
    18601 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    18661 aaatattgat ctatataccc ttccgcctga ccaaatgccg cttccagtac aaagttattt
    18721 ttgaagtcgt acttcgcacc gatggagtgc agataatcaa ctttggtagt tttatcgttc
    18781 tgataaaact catccatttc gagatgccac ggtgctttgt attcgttggt ccacatgtag
    18841 gagaaactca acgcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgcaccctga
    18901 taagtacccg gcataaagct ccagtgcggc gctaacagcg tctgaccagt tggctgaatg
    18961 taacctgccc tcgcccacac cggaccgtat ttaaatttgg ccgctgcttt atacaggctt
    19021 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattgctttt tgaaaacgcg
    19081 atttcgttcg ggtgagagct gtcaccgttt tccgccattt caatcgccgt aaacgcagca
    19141 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19201 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19261 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19321 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcgat agccagcgcc
    19381 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19441 ctgcaccaaa ggtgaacagc gaaaaaaata aaaattaaaa aagtagcgtt gagaaaagtc
    19501 tcgctgacta caggaacaga ctatttttcg cgacgcgaat tatcaatctc tttctgtaac
    19561 cagaatgtaa gaacatgagc aaccgcacat attttgatga tttttgttac aaggcttaag
    19621 tttgcagttg aaaggcttgc agaagaataa aagcgtgaat ttccagtaag atgaaaacgg
    19681 ttacatttta tttaattatg gtttgtgatt ttaaatttgt tttatggata aattgcgaag
    19741 cggaattaat tcgcgaagaa aag
//
LOCUS       DAZGAP010000100        19124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGAP010000100 DAZGAP010000000
VERSION     DAZGAP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.100"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..556
                     /gene="lpxT"
                     /locus_tag="ACHLAM_003035"
     CDS             <1..556
                     /gene="lpxT"
                     /locus_tag="ACHLAM_003035"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO7854529.1"
                     /translation="WLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLV
                     MLLTAVVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLL
                     IFSAFMWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLT
                     PLSDRLITFFDKSLPGKNKHFQNK"
     gene            968..1534
                     /gene="mepS"
                     /locus_tag="ACHLAM_003036"
     CDS             968..1534
                     /gene="mepS"
                     /locus_tag="ACHLAM_003036"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO7854530.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1715..3271
                     /gene="pdeN"
                     /locus_tag="ACHLAM_003037"
     CDS             1715..3271
                     /gene="pdeN"
                     /locus_tag="ACHLAM_003037"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7854531.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFTDLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            3353..5167
                     /gene="yejA"
                     /locus_tag="ACHLAM_003038"
     CDS             3353..5167
                     /gene="yejA"
                     /locus_tag="ACHLAM_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO7854532.1"
                     /translation="MIVRILLLFIALFTFGAQAQTIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNAGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRLEND
                     AKNWVTRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            5168..6262
                     /gene="yejB"
                     /locus_tag="ACHLAM_003039"
     CDS             5168..6262
                     /gene="yejB"
                     /locus_tag="ACHLAM_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO7854533.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            6262..7287
                     /gene="yejE"
                     /locus_tag="ACHLAM_003040"
     CDS             6262..7287
                     /gene="yejE"
                     /locus_tag="ACHLAM_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7854534.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            7289..8878
                     /gene="yejF"
                     /locus_tag="ACHLAM_003041"
     CDS             7289..8878
                     /gene="yejF"
                     /locus_tag="ACHLAM_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO7854535.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGDVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(8882..9226)
                     /gene="yejG"
                     /locus_tag="ACHLAM_003042"
     CDS             complement(8882..9226)
                     /gene="yejG"
                     /locus_tag="ACHLAM_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO7854536.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(9560..10750)
                     /gene="bcr"
                     /locus_tag="ACHLAM_003043"
     CDS             complement(9560..10750)
                     /gene="bcr"
                     /locus_tag="ACHLAM_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO7854537.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(10778..11473)
                     /gene="rsuA"
                     /locus_tag="ACHLAM_003044"
     CDS             complement(10778..11473)
                     /gene="rsuA"
                     /locus_tag="ACHLAM_003044"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO7854538.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11623..13383
                     /gene="radD"
                     /locus_tag="ACHLAM_003045"
     CDS             11623..13383
                     /gene="radD"
                     /locus_tag="ACHLAM_003045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO7854539.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAVTRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            13508..13792
                     /gene="rplY"
                     /locus_tag="ACHLAM_003046"
     CDS             13508..13792
                     /gene="rplY"
                     /locus_tag="ACHLAM_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO7854540.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(13931..14938)
                     /gene="yejK"
                     /locus_tag="ACHLAM_003047"
     CDS             complement(13931..14938)
                     /gene="yejK"
                     /locus_tag="ACHLAM_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO7854541.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            15120..15347
                     /gene="yejL"
                     /locus_tag="ACHLAM_003048"
     CDS             15120..15347
                     /gene="yejL"
                     /locus_tag="ACHLAM_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO7854542.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            15367..17127
                     /gene="yejM"
                     /locus_tag="ACHLAM_003049"
     CDS             15367..17127
                     /gene="yejM"
                     /locus_tag="ACHLAM_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO7854543.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            17202..17278
                     /locus_tag="ACHLAM_003050"
     tRNA            17202..17278
                     /locus_tag="ACHLAM_003050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17236..17238,aa:Pro,seq:ggg)
     gene            complement(17404..18163)
                     /locus_tag="ACHLAM_003051"
                     /pseudo
     CDS             complement(17404..18163)
                     /locus_tag="ACHLAM_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            18483..>19124
                     /gene="narP"
                     /locus_tag="ACHLAM_003052"
     CDS             18483..>19124
                     /gene="narP"
                     /locus_tag="ACHLAM_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO7854544.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGA"
BASE COUNT         4697 a         4862 c         4728 g         4837 t
ORIGIN      
        1 gtggctggtt gcattgacca acaatcgcgc tttcgacggt tgttcactgc tggcgatggg
       61 aatgttgatg ctgagtttct ggctgaaaga aaacgcccca ggcagacgac gtatcgtgat
      121 tattggtctg gtcatgctat taactgcagt ggtattaaac cagctgggtc aggcattaat
      181 tcctgtaaaa cgggccagcc caacattgac ttttaccgat attaaccgcg tcagcgaact
      241 gctctctgtt cccacgaaag atgcctcacg agatagcttt cccggcgatc acggtatgat
      301 gctgcttatt ttttcggcat tcatgtggcg ttatttcggc aaagttgcag gccttatcgc
      361 ccttattatt tttgtggttt ttgcgtttcc cagagtaatg attggcgcac actggtttac
      421 tgacatcatt gtcggttcga tgaccgtgat attgatcggt ttgccctggg tgttgctgac
      481 gccattaagt gatcgattaa tcaccttttt tgacaaatca ctaccgggaa aaaacaaaca
      541 tttccaaaac aaataactca cagtaattaa catcatcagg gttattttta tagtgaggat
      601 aatcctgatg atgcgcaccg tgctttcatc catcgcgcgc aaaaatcatt ctctaagtaa
      661 atgaatggat tgcatgcgtt tcactcaatc gtactttaat tgaccaatcc cgtttattaa
      721 ctttctgtat cactttttct tataaaaaat catgtaaaac cgctcgccaa gaccgcacca
      781 atcgggtaat ctcgaactcg ttttgcctcg gcggtagatt atcctcacag catataattt
      841 tgtgcgttag tccacagatt tggccttaag gaattgtttc aacatgccca ggtaattagt
      901 ctcgtgtcgc ttggcatttt tttataacga tatttgtcgt taaggacttc aagggaaaac
      961 aaacaacatg gtcaaatctc aaccgatttt gagatatatc ttgcgcggga tccccgcgat
     1021 tgcagtagcg gttctgcttt ctgcatgtag tgcaaataac accgcaaaga atatgcatcc
     1081 tgagacacgt gcagtgggta gtgaaacatc atcactgcaa gcttctcagg atgaatttga
     1141 aaacctggtt cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg ctgactggaa
     1201 aggtgtacgc tatcgtctgg gtggcagcac taaaaaaggc atcgattgtt ctggtttcgt
     1261 acagcgtaca ttccgtgagc aatttggctt agaacttccg cgttcgactt acgaacagca
     1321 ggaaatgggt aaatctgttt cccgtagtaa tttgcgtacg ggtgatttag ttctgttccg
     1381 tgccggttca acaggacgcc atgtcggtat ttatatcggc aacaatcagt ttgtccatgc
     1441 ttccaccagc agtggtgtta ttatttccag catgaatgaa ccgtactgga agaagcgtta
     1501 caacgaagca cgccgggttc tcagccgcag ctaataaacc gtttggatgc aatcccttgg
     1561 ctatcctgac gagttaactg aaagcactgc ttaggcagtg cttttttgtt ttcattcatc
     1621 agagaaaatg atgtttccgc gtcttgatcc aggctatagt ccggtcattg ttatctttta
     1681 aatgttgtcg taatttcagg aaattaacgg aatcatgttc atacgcgctc ccaattctgg
     1741 acgtaagctc ctgcttacct gcattgttgc aggcgtgatg attgcgatac tggtgagctg
     1801 ccttcagttt ttagtggcct ggcataagca cgaagtcaaa tacgacacac tgattaccga
     1861 cgtacaaaag tatctcgata cctattttac cgacctgaaa tccactactg accggctcca
     1921 gccgctgacc ttagatacct gccagcaggc taaccccgaa ctgactgccc gcgcggcgtt
     1981 tagcatgaat gtccgtacgt ttgtgctggt gaaagataaa aaaacattct gttcatctgc
     2041 gactggtgag atggacattc cactaaaaga attgattccg gcgctcgaca ttaataaaaa
     2101 tgtcgatatg gcgatcttac ccggtacgcc gatggtgccg aacaaacccg caatcgtcat
     2161 ctggtatcgc aacccgttgt tgaaaaatag tggcgtgttt gccgcgctga atctcaatct
     2221 gacgccttct ctcttttata gttcacggca ggaagattac gatggcgtcg ccctcattat
     2281 tggtaatact gcgctatcta ccttttcttc acgtttgatg aacgttaatg aattaaccga
     2341 catgccggtc cgtgaaacta aaattgcggg cattcctctg accgttcggc tttatgcaga
     2401 tgactggaca tggaacgatg tgtggtacgc atttttactg ggtggcatga gtggaacttt
     2461 cgttggactt ctctgctatt acctgatgag tgtgcgtatg cgcccaggca gagaaatcat
     2521 gaccgccatc aagcgcgaac aattttacgt ggtgtatcaa ccggtggtgg atacacaagc
     2581 tttgcgggta acgggcctgg aagtactgct acgctggcgg catccagtag caggagaaat
     2641 ccccccggat gccttcatta actttgccga agcgcaaaag atgattgtgc cactgactca
     2701 gcacctgttt gagttgattg cccgcgatgc cgcagaatta gaaaaagtac tgccggtagg
     2761 cgtcaaattt ggcattaaca ttgcgccggc ccacttgcac agcgaaagct ttaaagcgga
     2821 tatccagaaa ctgctcactt ccctgcccgc acaccatttc cagattgtgc tggaaattac
     2881 cgagcgcgat atgctgaaag agcgagaagc cacacaactc ttcgcctggc tgcattcggt
     2941 cggcgtagaa attgctattg atgacttcgg caccgggcac agcgcgctta tctatcttga
     3001 gcgttttacg ctcgattatc tgaaaattga tcgtggattt atcaacgcca tcggtacgga
     3061 aacgatcact tcacccgtac ttgacgcggt gctgacgctg gcgaaacgcc tcaatatgct
     3121 gacggttgct gaaggggtcg aaacgccaga acaggcacga tggctaagcg aacgcggcgt
     3181 taatttcatg caaggctact ggattagtcg cccgttaccg ctggacgatt ttgttcgctg
     3241 gctgaagaaa ccgtatacgc cgcagtggta aggtgtgctt acgtccctta ttattcatag
     3301 tgaaagcatg ccggattgcg gctaatgatg agtaaaagga aatccgttgc agatgattgt
     3361 gcgcatactg ctgctgttta tcgctctgtt cacctttggt gcgcaggcgc agactatcaa
     3421 ggaaagctat gcctttgccg tactgggcga accccggtac gcatttaatt tcaaccattt
     3481 tgattatgtg aaccccgccg cgccaaaagg tgggcaaata acgttgtctg cgctcggcac
     3541 cttcgataat ttcaaccgct atgcactgcg cggcaatgcg ggcgcacgca ccgagcagct
     3601 gtacgacacg ctatttacga cttccgatga cgaaccaggc agttattacc cgctgattgc
     3661 tgaaagcgca cgctatgctg acgattattc ctgggtggag gtcgctatta atccacgcgc
     3721 ccgttttcat gatggttcgc ccattactgc ccgcgatgta gagtttactt ttcaaaaatt
     3781 tatgaccgaa ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat
     3841 tgcgccgtta accgtgcgca ttgagttagc gaaacccggc aaagaagata tgctgagcct
     3901 gttttcgctg ccggtctttc cagaaaagta ctggaaggat cacaaactta gcgacccgct
     3961 cgccacgcct ccgcttgcca gtggtccgta ccgaattacg tcctggaaaa tggggcaaaa
     4021 tattgtctat tcccgcgtga aagattactg ggcagcaaac ttaccggtaa accgtggacg
     4081 ctggaatttc gacaccattc gctacgatta ttacctcgat gataatgtcg cctttgaagc
     4141 gtttaaagcc ggtgcctttg atttgcgttt ggaaaacgac gccaaaaact gggtcacgcg
     4201 ctataccggt aaaaatttcg ataagaaata catcatcaaa gatgagcaaa agaacgaatc
     4261 agcccaggat acgcgctggc tggcgtttaa tatccaacgt ccggtattca gcgatcgccg
     4321 ggtgcgggaa gcaatcactc tcgcctttga ctttgaatgg atgaacaagg cgttgtttta
     4381 taatgcctgg agtcgcacga acagttattt tcagaatacc gaatatgcgg ccagaaatta
     4441 ccccgacgcc gcggagctgg tgcttctggc accaatgaaa aaagatctac cgccagaagt
     4501 cttcacgcaa atctaccagc cgccggtatc caaaggcgat ggctacgatc gtgacaacct
     4561 gttaaaagcc gacaaacttc tcaacgaagc gggctgggtg ctgaagggtc agcaacgcgt
     4621 aaatgccaca acgggtcagc cgctcagctt tgagttattg ctaccttcga gcagcaatag
     4681 tcagtgggta ttgccgttcc agcacagcct gcaacggctg ggtatcaaca tggacattcg
     4741 caaagtggat aactcgcaaa tcaccaaccg catgcgcagt cgcgactatg acatgatgcc
     4801 gcgcgtatgg cgggcgatgc cgtggcccag ttccgattta cagatttcct ggtcatcgga
     4861 atatatcaat tccacttata atgcccccgg cgtgcaaagc ccggttatcg actcgctgat
     4921 caaccaaatt attgccgcgc agggaaataa agaaaaatta ctgccgttgg ggcgagcact
     4981 ggatcgcgta ttaacgtgga attattacat gctgccaatg tggtacatgg cggaagaccg
     5041 tctcgcctgg tgggataaat tctcccaacc cgctgtacgc cctgtttaca gcctgggtat
     5101 tgatacctgg tggtatgacg ttaataaagc gacgaaactg ccgtcagcca gacaacaggg
     5161 agagtagatg ggcgcttatc tgattcgccg tctgttgctg gtgatcccaa cgttatgggc
     5221 gattatcact atcaactttt tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc
     5281 catcgccgcc attgagtttg gtaatactgg tgtattaccc ggtgcaggcg gtgaaggtgt
     5341 tcgcgccagc catgcacaaa cgggcgtcgg taatatcagc gacagtaatt accgtggcgg
     5401 acgcggattg gatccagaag tgatcgccga gatcactcat cgctacggtt tcgataagcc
     5461 gatccacgaa cgttacttca aaatgctctg ggactacatc cgttttgatt ttggcgacag
     5521 cctttttcgc agcgcctcgg tgctgacgct gattaaagac agtctgccgg tctccatcac
     5581 cctcggattg tggagcacgc tgattatcta tctggtgtcg attccgttag gcattcgcaa
     5641 agctgtttat aatgggagcc gttttgacgt ctggagtagt gcatttatca tcatcggcta
     5701 cgccattccg gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta
     5761 tttcgacctg ttccctctgc gcggcctggt ttccgctaac tttgattcgc tgccgtggta
     5821 tcagaaaatc accgattatc tgtggcatat cacgctgccg gtgctggcga cagtgattgg
     5881 cggctttgcg gctctgacca tgctgacaaa aaactcattc cttgatgaag tgcgtaagca
     5941 atacgtggtg acggcacgtg cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt
     6001 gttccgcaac gccatgctgc tggtgattgc cggttttccg gcgacgttta tcagcatgtt
     6061 ttttaccggc tcgctgctga ttgaggtgat gttttcactc aatggtcttg gcttactggg
     6121 ctacgaagcg accgtctcgc gcgattatcc tgtaatgttt ggtaccttgt atattttcac
     6181 cctgattggc ctgctgctga atattgtcag tgatatcagc tatacgctgg tcgatccgcg
     6241 tatagatttt gagggacgtt aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg
     6301 cgttttcgtc ataaccgtcg cggctactgg tcgttatgga ttttcctcgt cttgtttggt
     6361 ttgagtttgt gttctgaact tatcgccaac gataaaccgt tgctggtgcg ttatgacggc
     6421 agttggtatt tcccgttgtt gaaaaactac agcgaaagcg attttggcgg cccgctggca
     6481 agtcaggctg attatcagga cccgtggctg aaacaacggc tggaaaataa cggctgggta
     6541 ctgtgggcac cgattcgctt tggtgctacc agtatcaact ttgctaccga taagcccttc
     6601 ccttctccac cctcccggca aaactggctg ggaacggatg ccaatggcgg cgatgtgctg
     6661 gcgcgcattc tctatggcac gcggatctcg gttctgtttg gcctgatgct gactctctgt
     6721 tccagcgtga tgggcgtgct ggcgggggcg ctacaaggct attacggcgg taaagtcgat
     6781 ctctgggggc aacgctttat tgaagtatgg tcagggatgc cgacgctgtt tttgattatt
     6841 ttactttcca gcgtcgtaca gcctaacttc tggtggctgc tggcaattac cgtcttgttt
     6901 ggctggatga gtctggtcgg cgtggtgcgg gcggagtttt tacgtacccg taatttcgac
     6961 tacatccgcg cggcgcaggc gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg
     7021 ttgccaaatg ccatggtcgc taccctcacc tttttaccgt ttattttatg tagttcgata
     7081 accaccctga cctcactcga tttcctcggc ttcggtctgc cgctcggttc accgtcactc
     7141 ggcgaactgc tgttacaagg gaaaaataac cttcaggccc cgtggcttgg gatcaccgcc
     7201 ttcttgtcgg tggcgatatt gttgtctttg ctgatcttta ttggtgaagc cgtccgcgac
     7261 gcatttgatc ctaataaggc ggtgtagcat gacgcaaact ctgttagcga ttgaaaattt
     7321 gtcggtgggt tttcgccatc agcaaaccgt acgcacagta gtcaatgatg tttcacttca
     7381 gattgaggct ggcgaaacgc tggcgctggt gggtgagtca ggttcaggca aaagcgttac
     7441 cgcgctgtca attttacgcc tgctcccttc cccgccggtt gaatatctct ccggcgatat
     7501 tcgttttcat ggcgaatcgc tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg
     7561 taataagatc gccatgattt ttcaggagcc gatggtgtcg ttaaatccat tacataccct
     7621 ggaaaaacag ctttatgaag tactttcact ccaccgcggg atgcgtcggg aagcggctcg
     7681 tggcgaaatt cttaactgcc ttgatcgcgt cggtatccgc caggcggcaa aacggctgac
     7741 agattatccg catcagctct ccggcggcga acgccagcgg gtgatgattg cgatggcgct
     7801 gttaacgcga ccggaattat taattgccga tgaaccaacc accgcgctgg acgtctctgt
     7861 ccaggcgcag attttacagc tgttgcgcga actgcaaggc gagctgaata tgggtatgct
     7921 gtttattact cataacctca gcattgttag aaaactggcc caccgcgtgg cggtaatgca
     7981 aaacggtcgc tgtgtcgagc aaaataacgc cgctacgcta tttgcctcac ccactcatcc
     8041 ttacacacaa aagctactca acagtgaacc gtcaggcgac ccggtcccat tgccagaacc
     8101 tgcctcaacg ttgctggatg ttgaacagct tcaggttgcc ttccccattc gcaaagggat
     8161 tttgaagcgc attgtggatc ataatgtggt ggtgaaaaac atcagtttta cgctacgagc
     8221 gggtgaaaca ctgggtttag tgggcgagtc tggttccggg aaaagcacga cgggactggc
     8281 gctgctgcga ctgattaact ctcagggcag catcgtcttt gacggtaagc cactgcaaaa
     8341 tttaaatcgc cgccagctgt tacctattcg tcatcgcatt caggtggtat ttcaggatcc
     8401 aaactcctcg ctcaacccac gactcaacgt tttgcagatt attgaggaag gcttacgggt
     8461 tcaccagccg acgctttctg ccgcgcaacg cgaacaacaa gtgatagccg tgatgcatga
     8521 agtgggatta gatcctgaaa cacgccaccg ttatccggcg gagttctctg gtggtcagcg
     8581 gcaacgtatt gcgattgcca gggcgttaat tcttaagccc tcgctgatca ttcttgatga
     8641 accaacatca tcactcgaca aaacggtgca ggcgcaaata ttgacgctat tgaaatcatt
     8701 gcaacaaaag catcaactgg cctatttgtt tatcagccac gatttgcacg ttgtccgcgc
     8761 gttatgtcat caggttatcg tactgcgaca aggggacgta gtggaacaag gaccgtgcgc
     8821 gcgcgtgttt gccgcaccgc agcaggagta tacgcgtcag ctactggcgt tgagctgacg
     8881 cttaaaaagg attgtagttt gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc
     8941 ggcatgttgc agcaaattcg tcctggcgat ttacaaacag gcacggctca ccttcacact
     9001 ccagcaccga acatggaact tcgatgtcgc ttaacgcctg gcttagtttg tacatcaccg
     9061 accatgcatt atcaccatcc gggctaagca atttaagcgc caccaggctg ttgtcaccgc
     9121 acggtccatt ttgcggaatc ggttcaacct tcgaacctgc gaaaccagca gaccagcgat
     9181 agttctgcgg cagtcgatgg acgattgaga gttgtaatga agtcacttat tttccccgga
     9241 agtacatttt acttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct
     9301 ttatacagat cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc tgatttaaat
     9361 aatatctgcg ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact
     9421 caaccttttt aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac
     9481 atatatcggg ggaatgatag atttgtggtt gacatgcatc aacaaaggaa gccttttagc
     9541 ttcctcgttg tgcaatagat caccgttttt tcggccgact ggcgtacaaa cagaagagaa
     9601 tggagctggt tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg
     9661 caagagaaag taatgcgcca acaattgccc ctatgccgaa acggaaggtt cctgccagcg
     9721 aagatgccgt tcccgccata tggggaaact catcaagaat gaccgccatc gcattggatg
     9781 acaccatcga cacgcagccc acaaacgccg caacgccaac caccagcgac cagaatccca
     9841 gccccagcag tgcactgatg accatccacg ctgccataat aaactgtatc cacaaccccg
     9901 agcggaacat atttaacgcg ccaatacggc ggacgaagcg gctgttaaag atggtcatca
     9961 cgaacagaaa aagaatgttt agcgcaaagt aataaccaaa gttttccggc gcgacgtggt
    10021 tgatttcaat gtagacaaac ggtccggcgc ttaagaatga gaacatcccg gcaaagctga
    10081 aaccgctggc cagcatataa ctcaggacac gcttatgacg gaacagcgcc gcaaagttac
    10141 caatagtggt acgaatgtga aaaggctggc gacgctccgg tggtaaggtt tctttgatca
    10201 gaaagaagat cattgccgaa gccagaatcg ccgccacggc gaggatccag aagatgtaat
    10261 gccagctcag ccacaccagc acccagccgc caactatcgg tgccatcagc ggtgcaatgg
    10321 tcgtcaccag catgacaaac gacatcatcc gtgagaactc ttctttcggg tagatatcac
    10381 gcatcaaagc gttgataacc acgctggccg cagccgcagc cagcccgtgg aagaaacgca
    10441 tcacaatcag ctgatcgatg gtttgtgcca acgcacacgc caccgcggcg gcggcaaaca
    10501 ccagcgtacc gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga
    10561 ttaactgccc caacgcaaag cccagaatat aagtactgag ggtcatctgc gtactgcccg
    10621 ccggtacacc aaactgagct gaaattaccg gtagcgcagg cagatacata tcaatcgaca
    10681 gcggcatcaa catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc
    10741 gggtggtcac aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt
    10801 cttcagttaa cggacgatat tcaccgggag ctaaatcagc atccagcgta atgccgccaa
    10861 tacgttcacg atgcagctca accacatggt tacccacggc ggcgaacatg cgtttcacct
    10921 gatgataacg cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttccagca
    10981 ccgccggctt agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg
    11041 ccgtatcgtc agctacgggt gattccagcg tcaccagata ggtcttctcg caatgatggc
    11101 gcggggaagt aatgcggtgc gaccactgac catcatctgt cattagtacc agaccggtgg
    11161 tatcgatatc cagacgccct gcagcgtgca gtttccacgc taccggttca tcaagaaaat
    11221 agagcaccgt tgggtgatcc gggtcgtccg tggagcaaac atagccctga ggcttattga
    11281 gcatgaagta acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat
    11341 gttcagggag cagtttgaac gctgcattac ggacgatttc gccatcgacg gtgacacgat
    11401 tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt
    11461 tatcaagtcg catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa
    11521 aatctgaact gttgtctgcc cagtatagcg gtctaacaac atccctcaag ggaaaaagat
    11581 ccatggcata ctattagcag aataatctac ctacgcgaga ccatgatttt tacacttcgc
    11641 ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct
    11701 gccgttatcg tgctgcccac cggtgcaggt aaaagcctgg tgatagcgga actggcgcgg
    11761 ctggcgcgtg gtcgcgtgct ggtgctggca cacgttaaag aactggtggc gcaaaaccat
    11821 gcaaaatatc aggcgctggg gctggaagcc gatatttttg ccgccgggct aaagcgcaaa
    11881 gagagccacg gtaaagtggt atttggcagc gtgcagtcgg tcgcccgtaa tcttgatgcc
    11941 tttcagggtg aattttcgct gttgattgtc gatgaatgtc accgtattgg tgacgatgaa
    12001 gagagccagt atcagcaaat cctcactcac ctgaccaaag tgaatcccca cttacgcctg
    12061 ctggggctga ctgccacgcc ttttcgactg ggcaaaggct ggatttatca gttccattat
    12121 cacggcatgg tacgcggcga tgagaaagcc cttttccgtg actgcattta tgagctaccg
    12181 ctgcgttata tgattaaaca cggctatctg acgccgccag aacgactgga tatgccagta
    12241 gtgcaatacg atttcagccg cttgcaggca cagagtaacg ggctattcag cgaagccgat
    12301 ctcaaccgcg agctgaaaaa acagcaacgc attaccccgc acatcatcag ccagattatg
    12361 gagtttgctg taacgcgcaa aggggtgatg attttcgccg ccaccgttga acacgcaaaa
    12421 gagattgtgg gactgctgcc tgccgaagat gctgcactga ttactggcga cacccccggc
    12481 gctgagcgcg atgtgttaat tgaagatttt aaagcccagc gttttcgcta tctggtcaac
    12541 gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc
    12601 cctaccgaat cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg tctcgctccc
    12661 ggcaagactg attgcttaat tcttgattat gcgggtaacc ctcacgatct ctacgcgccg
    12721 gaagttggta caccgaaagg caaaagtgac aacgttccgg tacaggtttt ctgccctgcc
    12781 tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt gattgaacac
    12841 tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg gacatcgcga acagtgtgac
    12901 ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgctg aaaatgatat tgccgcccgc
    12961 cgctgccgcg aatgtgacac ggtactggtt gatccggatg atatgttaaa agcggcgcta
    13021 cgactgaaag acgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac
    13081 gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggcgcgga tgtgagtgag
    13141 cgtttccgtc tgcaaacgcc cgcccagcgt accgccttcg aacaactttt tatccgtccg
    13201 catacgcgca caccgggcat ccctctgcgc tggatcaccg ccgccgatat cctcgcccag
    13261 caagcattac tgcgacaccc ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa
    13321 gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt
    13381 taatggtagt tttgattgat gtataaggcg attgagtata gaatctcgcc cgcttttgca
    13441 tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga
    13501 gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg
    13561 ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcgccgct
    13621 ggctgtcgaa ctggatcacg acaaagtcat gaacatgcaa gttaaagctg aattctacag
    13681 cgaagttctg accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca
    13741 gcgtcacccg tacaaaccga agctgctgca catcgacttc gttcgcgctt aattgctgaa
    13801 taagttgtaa aaaaccccgc tccggcgggg ttttttgtct ctgcaaatta tgcctgatta
    13861 cggtattgct attttttgca ggccagataa ggcgttcacg ccgcatctgg cattcagaac
    13921 acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa ttgatcgcgc aagttcggcg
    13981 gcgtgccttt aatggtcagg gtatcggttg ctggatccca gaagatgcgt tcacctaaca
    14041 gcatcgcatc aaagttaata gtcaagccac cgccgctacc ggcaaacttc gtcaactgac
    14101 gcagtgtgct acggtctgcc gggaagctct cttccagttc gtagcctttt tcagccgcaa
    14161 actccgtgaa gctcacttcg ctgacgcctg ccagctcttt cgacagcgat tccagttcaa
    14221 tctcttcacc tgcctgcagc tgctcattac agtagctata aacctgctgg cgcacgttct
    14281 gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc atcgaccgct tgcaacagcc
    14341 cacggttttg cgctttcgcg tttaaccctt cgctggcacc gaggaaatcc ataaagaaat
    14401 ccgccacttt gcgccctact cgccctttca ggaaagtgag atagcgggtc gactctggat
    14461 tggtttccca ctcggttaaa tcgatacgcg caacaatatc cgcatgattg atatcaagat
    14521 aatgggttgg gttgatgtcg agattttcgt taacacgcat actgctcagg ttgctcagaa
    14581 ctgccaccag cagatactcc accgccagat agcgatagtg gcaaaacagc acgaatccac
    14641 cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg accggttgcc gcgcggctaa
    14701 atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc agttcactct
    14761 cttcgctaaa caagccgtaa gctttatttt tggcgctata gacccgatgc agttctgcca
    14821 ccatctcaac gacggtttcc gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt
    14881 tctgctcatc acgcttgata agctggtgca gagcaatctg gttgatatcc agactcatga
    14941 taaactctcc tttaagaccg ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa
    15001 gatggcggtc attttacaga aggcggcgta acgagaatga tttaacgcat aaaaaagcag
    15061 aaaaaaaacc gttgctacgg taatatgttg ccctttcatc aactaactga tttcgattta
    15121 tgccacaaat ttcccgctac agtgatgaac aggttgaaca attgctcgca gagctgctca
    15181 acgtactgga aaaacataag gctccgaccg acctttccct gatggtgtta ggcaacatgg
    15241 tcactaacct tatcaacacc agcattgccc cggcccaacg ccaggcaatt gccaactctt
    15301 ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc gcactaaggg aaacagataa
    15361 caggttatgg taactcatcg tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg
    15421 gggcactggt ttgcactgtt caatattctg ctttcgctcg tcattggcag ccgttacctg
    15481 tttatcgccg actggccgac aacgcttgct ggtcgcattt attcctacgt aagcattatc
    15541 ggccatttca gcttcctggt gttcgccacc tacttgctga tcctcttccc gctgaccttt
    15601 atcgtcggct cccagaggct gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg
    15661 acgctattac tgatcgatag cgaagtcttt actcgtttcc atctccatct taatcccatc
    15721 gtctggcaac tggttatcaa cccagacgaa aatgagatgg cgcgcgactg gcagctgatg
    15781 ttcatcagcg tgccggttat tttattgctt gaactggtgt ttgcgacgtg gagctggcaa
    15841 aagctacgca gcctgacgcg tcgtcggcgc ttcgcgcgcc cgctggccgc attcttattt
    15901 atcgccttta tcgcctcgca tgtggtttat atctgggccg atgccaactt ctatcgcccg
    15961 atcaccatgc aacgcgctaa cctgccgctt tcgtacccga tgacggcgcg acgttttctt
    16021 gagaagcatg gtctgcttga tgcgcaggag tatcaacgcc gtctcattga gcaaggtaat
    16081 ccagacgccg tttccgttca gtatccgtta agcgaactgc gctatcgcga tatgggcacc
    16141 gggcagaatg tgctgttgat tactgtcgat ggcctgaact actcacgctt cgagaagcag
    16201 atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc
    16261 ggcaacacta cagacaacgg catctttggc ctgttctatg gcatctcgcc gagctatatg
    16321 gatggcattc tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc
    16381 tatcagctgg ggttattctc gtcagatggc tttaccagcc cgctgtatcg gcaggcattg
    16441 ttgtcagatt tctcgatgcc gagtgtacgc acccaatccg acgaacagac cgccacgcag
    16501 tggatcaact ggctgggccg ctacgcacaa gaagataacc gctggttctc gtgggtctct
    16561 ttcaatggca caaacattga cgacagcaat cagcaggcat ttgcacggaa atatagccgg
    16621 gcggcaggca atgtcgatga ccagatcaac cgcgtgctca atgcactgcg tgattctggc
    16681 aaactggaca atacggtggt gattatcact gccgggcggg gtattccgct gagcgaagag
    16741 gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc cattagtgat tcactggcca
    16801 ggcacgccgg cgcagcgtat taatgcgctg actgatcata ccgatctgat gacgacgctg
    16861 atgcaacgcc tgctacatgt cagcacacct gccagcgaat attcgcaagg tcaggatttg
    16921 ttcaaccctc aacgccgcca ttactgggtt accgccgcgg ataacgatac gctggcaatt
    16981 accaccccga aaaagacact ggtgctgaac aataacggta aataccgcac ttacaactta
    17041 cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt tgttattgca agtactgaca
    17101 gacgagaagc gttttatcgc taactgatta attataaatc agttagcaaa atatcttact
    17161 tgcaatcggt gtggaaaacg gtagtattag cagccacgag tcggcacgta gcgcagcctg
    17221 gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa
    17281 aatcccaaga aaaccaacct attgcggttg gtttttttat atctgcattt aattcgataa
    17341 acagacagcg acacatcacg acctgtttat tttctgttat cagaacgtcc agactacacc
    17401 cgcctgagtt gtgctgcttt cttcacggga gaaacgacta tcgataccga gcgtcaggcc
    17461 cgtcaatgtt tgctcaaagc cagctcccgc atcagtgttc acgttgttgc ggctgttaat
    17521 tgccgaactc cacatcgccg ccctttacgc taccaagacg ctcacgcacg gtgtccagtt
    17581 ctgcatcaaa taccagcggt tgtgctgccg ccatattcag cacatccgtg gttgaagggg
    17641 gcatttgtac gcgattctct gtgagactcc agctatggtt gccattatcc agcaaggtat
    17701 attcatacgt actgatatca acaacgcttc cggcattgcc caacgtaaat gccgcatcac
    17761 cgcgccctcc tttaatcgtg ggatcagtcg ccgcgccata aacctgtagc actccattat
    17821 tgatcgtagt ctggtttacc gcgccaccca tttgaacaat ttgataacca tcatttaata
    17881 ctgagtattc tgcggtgcca tttatttcaa tgtactgata tcccccgtta attttagtat
    17941 tactacttac accaaattct tatatatgct gttctccgcc aggattgatt ttcgtattat
    18001 tggcggcccc ataaaccagc tggatatctt tttccaggac aacaccatca actgtctcac
    18061 catactcaac agttgatgcc aatgctatgg gtgaatataa cgctgtagcc accagaacag
    18121 atattgcgga acgacaaaga gaaacagaac cagattggtg cattaagctt tcatcctatg
    18181 aaattaattg ctgttaaaag cattggggac agaaaatacc catagctcca tacccggagt
    18241 cagttttaaa aaactgttta aagaaatgca caagtattgt gattgatttt ttagttgttt
    18301 ttcctgatga gaagctgatg caaaattccg tctttataat gaagatgatg ccaaagcgaa
    18361 cgacaaagtt gtagttttca ctacatgtcc atacataaaa tggggtaaca ttcacgcgcc
    18421 tggtagcgtt accaacgcta cgctcaaaca taatgattct aataaaacct caggagacta
    18481 ctatgcctga agcaacgcct tttcaggtga tgattgtgga tgatcatcca cttatgcgac
    18541 gcggtgttcg tcagttactg gagcttgatc ctggctttga agtggtcgcc gaagcgggcg
    18601 acggcgcgag cgcgatcgat ctggcgaata gactggatat cgacgtgatc ttgctggatc
    18661 tcaatatgaa aggtatgagt ggcctggata ccctcaatgc cttgcgcagg gatggcgtta
    18721 ccgcgcaaat tattatcctg accgtatccg atgcctccag cgatgtcttt gcgctgatag
    18781 acgcaggcgc agacggttat ctgttgaaag acagcgaccc ggaagtgttg cttgaagcga
    18841 ttcgtgccgg agcgaaaggc agcaaagttt ttagcgaacg cgtcaatcag tacttacgtg
    18901 aacgtgaaat gtttggcgcg gaagaagatc ccttcagcgt gctgacggag cgcgagctgg
    18961 atgttctgca cgagctggca caggggctgt caaataaaca gattgcctcg gtgttgaata
    19021 tttccgagca gacagtaaaa gtacacattc gcaatctgct gcgtaaactc aatgtccgct
    19081 cacgcgtggc ggccaccatt ctgttcctgc aacaacgcgg ggca
//
LOCUS       DAZGAP010000101        18939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGAP010000101 DAZGAP010000000
VERSION     DAZGAP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.101"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..782
                     /gene="yqfA"
                     /locus_tag="ACHLAM_003053"
     CDS             123..782
                     /gene="yqfA"
                     /locus_tag="ACHLAM_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO7854545.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(859..1839)
                     /gene="ygfZ"
                     /locus_tag="ACHLAM_003054"
     CDS             complement(859..1839)
                     /gene="ygfZ"
                     /locus_tag="ACHLAM_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO7854546.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            2092..2358
                     /gene="sdhE"
                     /locus_tag="ACHLAM_003055"
     CDS             2092..2358
                     /gene="sdhE"
                     /locus_tag="ACHLAM_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO7854547.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            2339..2746
                     /gene="ygfX"
                     /locus_tag="ACHLAM_003056"
     CDS             2339..2746
                     /gene="ygfX"
                     /locus_tag="ACHLAM_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO7854548.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(2786..3307)
                     /gene="fldB"
                     /locus_tag="ACHLAM_003057"
     CDS             complement(2786..3307)
                     /gene="fldB"
                     /locus_tag="ACHLAM_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO7854549.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            3419..4315
                     /gene="xerD"
                     /locus_tag="ACHLAM_003058"
     CDS             3419..4315
                     /gene="xerD"
                     /locus_tag="ACHLAM_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO7854550.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            4340..5050
                     /gene="dsbC"
                     /locus_tag="ACHLAM_003059"
     CDS             4340..5050
                     /gene="dsbC"
                     /locus_tag="ACHLAM_003059"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO7854551.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            5056..6789
                     /gene="recJ"
                     /locus_tag="ACHLAM_003060"
     CDS             5056..6789
                     /gene="recJ"
                     /locus_tag="ACHLAM_003060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO7854552.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHSGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGVA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAL
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLTYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6880..7978
                     /gene="prfB"
                     /locus_tag="ACHLAM_003061"
     CDS             join(6880..6954,6956..7978)
                     /gene="prfB"
                     /locus_tag="ACHLAM_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO7854553.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7988..9505
                     /gene="lysS"
                     /locus_tag="ACHLAM_003062"
     CDS             7988..9505
                     /gene="lysS"
                     /locus_tag="ACHLAM_003062"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7854554.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(9548..10096)
                     /gene="idi"
                     /locus_tag="ACHLAM_003063"
     CDS             complement(9548..10096)
                     /gene="idi"
                     /locus_tag="ACHLAM_003063"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO7854555.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            10151..10222
                     /locus_tag="ACHLAM_003064"
     CDS             10151..10222
                     /locus_tag="ACHLAM_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854556.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(10219..10344)
                     /gene="yqfG"
                     /locus_tag="ACHLAM_003065"
     CDS             complement(10219..10344)
                     /gene="yqfG"
                     /locus_tag="ACHLAM_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO7854557.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(10346..11794)
                     /gene="uacT"
                     /locus_tag="ACHLAM_003066"
     CDS             complement(10346..11794)
                     /gene="uacT"
                     /locus_tag="ACHLAM_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO7854558.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            12229..14148
                     /gene="ygfT"
                     /locus_tag="ACHLAM_003067"
     CDS             12229..14148
                     /gene="ygfT"
                     /locus_tag="ACHLAM_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO7854559.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            14148..14636
                     /gene="ygfS"
                     /locus_tag="ACHLAM_003068"
     CDS             14148..14636
                     /gene="ygfS"
                     /locus_tag="ACHLAM_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7854560.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(14672..16039)
                     /gene="ghxQ"
                     /locus_tag="ACHLAM_003069"
                     /gene_synonym="ygfQ"
     CDS             complement(14672..16039)
                     /gene="ghxQ"
                     /locus_tag="ACHLAM_003069"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO7854561.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLILSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(16075..17391)
                     /gene="guaD"
                     /locus_tag="ACHLAM_003070"
     CDS             complement(16075..17391)
                     /gene="guaD"
                     /locus_tag="ACHLAM_003070"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO7854562.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(17409..18809)
                     /gene="xanQ"
                     /locus_tag="ACHLAM_003071"
     CDS             complement(17409..18809)
                     /gene="xanQ"
                     /locus_tag="ACHLAM_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO7854563.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGATLQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
BASE COUNT         4798 a         4729 c         4987 g         4425 t
ORIGIN      
        1 catgattttt cggagattag tctcaaatca gaatttattt tagctaacag gtgttcactg
       61 gaactattct cagttacgct ggagaggtat acatgtgcgt gtacgtttct ccggagtaag
      121 ttatggttca gaagcccctc attaagcagg gatattcgct ggcagaggaa attgccaaca
      181 gcgtcagtca cggcattggg ttggtgtttg gtatcgttgg gctggtgttg ctactggttc
      241 aggcggtgga tctcaatgcc agcgccacag cgataaccag ttacagcctc tatggcggca
      301 gtatgatcct gctgttcctc gcttcgacgc tctatcacgc cattcctcat caacgggcaa
      361 aaatgtggct gaagaaattt gaccattgcg ctatttacct attgattgcc ggaacctaca
      421 cgccgttttt gctggtgggg ctggattctc cgttagcgcg cgggttgatg attgttatct
      481 ggagcctggc attgctgggg attctgttca aactgaccat cgcgcaccga ttcaaaattt
      541 tatctctggt gacctatctg gcgatgggct ggctgtcgct ggtggtgatt tatgaaatgg
      601 caattaagct tgcggcgggc agcgttacct tactggctgt aggcggcgtg gtttactcgc
      661 tcggggtgat tttctacgtc tgcaaacgca ttccctacaa ccatgccatc tggcacggct
      721 ttgtgctcgg cggcagcgtg tgccactttc tggcgatcta tttatatatt gggcaggcgt
      781 aatgggtgtg cctttgtagg cctgaaaaga cacacccttg tcgcatcagg cgataaatgc
      841 atgatgcgac acagagattt actcttcgag tgaatacggc agcggctcga tatgcaatgt
      901 attcgcatcg tcacgcacgc ggaagatgct gtccggttcc atatcgttat tcatgacgac
      961 ctgtaccacg acctgaccat cttccagttt taccgcagcc agcacggttc cggtacgacg
     1021 ccagttctcg cccattttca gctctaagtc ttcaccggct tccggcagtc ggctggcgct
     1081 acccgtcagc aaccagagcg cgcgtttgtt ggcaccacgg aatttagctc gcgccaccat
     1141 ctcttgtccg gtatagcagc cttttttaaa gctaatgccg cccagcgcct ggaggttggt
     1201 cgcctgtggg ataaactgcc cgctgttggc ggcatcaatc accgggaaac ccgcttcaat
     1261 gtttaatgcc agccactgtt ggctattgtt cagttccgct tcaccgcgca gtttatcggt
     1321 cagcatattg gcagtggctt catcggttac gatcaggaaa cgttctgccg ggtgttcaaa
     1381 ccatagcaaa gtggtcgcgc cttctttaac tacctgtttt tctttcgaag gcagttcact
     1441 aaagatattt gccagcgcgg cgcgcgcctg aaaaccggca acaccaagca gcacacgctc
     1501 gtcgtctggc gcgatggtca ctttagagaa caccgcatat tttttcagtt cagtcagctg
     1561 cggttcacgc acgctgcgcc gttcaatcca cgcaaagcca tcgccgtcgc ggaacagacg
     1621 taaattgctc cacattttgc ctttggcgtc gcaatgggcg gcgagcaggt gctgatcttc
     1681 tgtcatctgg ctgacatctg ctgtcacctg accctgcata tatttttcgc tgtccgcgcc
     1741 agtaatggtg gcaagcgccc agtcatcaag cgtcatcagc gtcagcggta aacgggcaga
     1801 agccgtaggc tgacggggag gaaaaggtgt aaaagccata atagagtcct gagttgctta
     1861 acggcgtatt aatgactaat ggtaaaagag ctatgaggca atgcaagtgc tttcactacg
     1921 cgatctgtgc cttctgatat gctttgcgag gcgacttcca aaaaaatgaa gcccttgaat
     1981 tgttgcgaat taatttcgcg atcgcgattg tgtttactga tattcccgca aaagaagtcg
     2041 aaaaacacgt tacaatagcc gggtactgga ttttcgtgag acaggaagaa gatggacatt
     2101 aacaacaaag cccgcattca ttgggcatgc cgccgtggta tgcgcgaact cgatatttca
     2161 atcatgccgt ttttcgaaca tgagtacgac agcttaagcg atgacgaaaa acgcatcttt
     2221 attcgtctgc tggaatgcga cgacccggac ctgtttaact ggctgatgaa tcacggtaaa
     2281 ccggccgatg cagaactgga aatgatggtc cgactcatcc agacacggaa ccgggaacgt
     2341 ggtcctgtgg caatctgatt tgcgcgtctc ctggcgcgca cagtggcttt ccttgctgat
     2401 tcatgggctg gttgccgctg ttattttact catgccctgg ccgctcagtt acaccccgtt
     2461 atggatggtg ttgctttcgc tggtggtgtt tgattgcgtt cgcagccagc gccgcattaa
     2521 tgctcgccag ggggaaattc gcctgttgat ggacgggcgt ttgcgttggc aagggcagga
     2581 gtggagcatc gtcaaagcgc cgtggatgat taagagcggc atgatgctgc gtttacgttc
     2641 tgacagcggt aaacggcaac atttatggct ggcagccgac agcatggacg aagctgaatg
     2701 gcgggattta cggcggattt tgttgcaaca agagacgcaa agataaagat gacggcaaag
     2761 catatgcgct aagccgtcag tgacatcagg cgtaatgctc tgccatttcg ttgaggattt
     2821 gctcgcacca gctctgaata cgctcgtcgc taaggtcata ctggttagtt tcatccagcg
     2881 ccagacccac gaacagttgc ccgtcagcaa tcaccggttt cgggctggta aattcatatc
     2941 cttccgttgg ccagtagccg acgaacttca cgcctttggt cgagagtttg tcatgcagca
     3001 taccgagcgc atcgaggaac cactcgccgt atcccagttg atcgccaagc ccatacagcg
     3061 caacaatttt accttcgagg ttcaggtcgt cgagctgatc ccagacggct tcccagtctt
     3121 cctggatttc gccaaaatcc caggtcggga tacccagaat gagcacatcg tactgctcca
     3181 ttaatttcgg ggagtcgtcc ttgaggttat gtaaggtcac cagttctggg ccgataatat
     3241 cgcggatttt ttctgccgcc atttcggtgt aacaggtgct ggaaccgtaa aaaagaccca
     3301 tattcataac gtaagcttct cgattctgct gtggcttttg cggctagtgt atcagaatcg
     3361 ctttagtcac ggcacaataa ggcataatgg atcaaatgag tgaccataag gggcgcaagt
     3421 gaaacaggat ctggcacgca tcgagcagtt tcttgatgct ctgtggctgg aaaaaaatct
     3481 ggctgaaaat acgttgaacg cttaccgtcg cgatctgtca atgatggtgg agtggttgca
     3541 tcaccgtggg ttgacgctgg cgacggcgca aagtgacgat ttgcaggcat tactggcaga
     3601 acggctggag ggcgggtata aagccaccag ctcagcgcgt ttgctgagtg cagtgcgccg
     3661 attgttccag tatctttatc gcgaaaagtt tcgtgaagat gatcccagtg cgcatctcgc
     3721 ttcaccgaaa ttgccccagc gtttgccaaa agatttaagc gaagcgcagg tcgaacgttt
     3781 attacaggca ccattaattg atcagccact ggagctacgc gataaagcca tgcttgaagt
     3841 gttgtatgct accggactgc gtgtctctga actggtcggg ctgacaatga gtgatatcag
     3901 cctgcgtcag ggcgtagtgc gggtcattgg taaaggcaac aaagagcgcc tggtgccgtt
     3961 aggtgaagag gcggtttact ggctggaaac ctatctggaa catgggcgtc cgtggctgtt
     4021 gaatggtgtg tcaattgacg tgttgtttcc cagccagcgt gcgcagcaga tgacgcgaca
     4081 gaccttctgg caccgcatta aacattatgc cgtgctggcg ggtatcgaca gcgaaaagct
     4141 gtcaccgcat gtgttgcgtc acgcttttgc cactcactta ttaaatcatg gtgcggattt
     4201 acgcgtggtg cagatgctac tgggccacag cgatctctcc accacgcaaa tttatacgca
     4261 tgtcgctacc gagcgtctgc gacaacttca tcaacagcat cacccgcggg cgtgatgtct
     4321 gaaaagaaca ggaaaattta tgaagaaagg ttttatgtta tttaccttgt tggcagcgtt
     4381 ttcaggcttt gttcaggctg atgacgcggc aattcaacaa acgttagcca aaatgggcat
     4441 caaaagcagc gatattcagc ccgcgcccgt agctggcatg aagacagttc tgactaacag
     4501 cggtgtgttg tacatcaccg atgatggtaa acatatcatt caggggccaa tgtatgacgt
     4561 tagtggcacg gctccggtca atgtcaccaa taagatgctg ttaaagcagt tgaatgcgct
     4621 tgaaaaagag atgatcgttt ataaagcgcc gcaggaaaaa cacgtcatca ccgtgtttac
     4681 tgatattacc tgtggttact gccacaaact gcatgagcaa atggcagact ataacgcgct
     4741 ggggatcacc gtgcgttatc ttgctttccc gcgccagggg ctggacagcg atgcagagaa
     4801 agaaatgaaa gctatctggt gtgcgaaaga taaaaacaaa gcgtttgatg atgtgatggc
     4861 aggtaaaagc gtcgcaccag ccagctgcga cgtggatatt gccgaccatt acgcacttgg
     4921 cgtccagctt ggcgttagcg gtactccggc agttgtgctg agcaatggca cacttgttcc
     4981 gggttaccag ccgccgaaag acatgaaaga attcctcgac gaacaccaaa aaatgaccag
     5041 cggtaaataa ttcgcgtgaa acaacagata caacttcgtc gccgagaagt cgatgaaacg
     5101 gccgacttgc ccgctgaatt gcctcccttg ctgcgccgtt tatatgccag ccggggtgtg
     5161 cgtagcgcgc aggaactgga acgcagtgtt aaaggcatgc tgccctggca gcaactgagc
     5221 ggcgtcgaaa aggccgttga gatcctttac aacgcttttc gcgaaggcac gcggattatt
     5281 gtggtcggtg atttcgacgc cgacggtgcc accagcacgg ctctaagcgt gctggcgatg
     5341 cgctcgcttg gttgcagcaa tatcgactac ttggttccaa accgtttcga agacggttac
     5401 ggcttaagcc cggaagtggt cgatcaggcc catgcccgtg gcgcgcagtt aattgtcacg
     5461 gtggataacg gtatttcctc ccattcgggc gttgaacacg ctcgctcgtt gggcattccg
     5521 gttattgtta ccgatcacca tttgccgggc gaaacattac ccgcggcgga agcgatcatt
     5581 aaccctaact tgcgcgactg taatttcccg tcgaaatcac tggcaggcgt gggtgtggcg
     5641 ttttatctga tgctggcgct gcgcaccttt ttgcgcgatc agggctggtt tgatgagcgc
     5701 ggcgtcgcaa ttcctaacct ggcagaactg ctggatctgg tcgcgctggg aacagtggcg
     5761 gacgtcgtgc cgctggacgc caataaccgc atcctcacct ggcaggggat gagtcgcatc
     5821 cgtgccggaa agtgccgtcc agggattaaa gcgctgctgg aagtggcaaa ccgtgatgca
     5881 ctaaaactcg ccgccagcga tttaggtttt gcgctggggc cacgtctcaa tgctgccggg
     5941 cgactggacg atatgtccgt cggtgtggcg ctgttgctgt gcgacaacat cggcgaagcg
     6001 cgcgtgctgg caaatgaact cgatgcgcta aaccagacgc gaaaagagat cgaacaagga
     6061 atgcagattg aagccctgac cctgtgcgag aaactggagc gcagccgcga cacgttaccc
     6121 ggcgggctgg caatgtacca ccccgaatgg catcagggcg ttgtcggtat tctggcttcg
     6181 cgcatcaaag agcgttttca ccgtccggtt atcgcgtttg cgccagcagg tgacggtacg
     6241 ctgaaaggtt ccggtcgctc cattcagggg ctgcatatgc gtgatgcgct ggagcgatta
     6301 gacacactct accccggcat gatgctcaag tttggcggcc atgcgatggc ggcgggtttg
     6361 tcgctggaag aggataaatt cgaactcttt caacaacggt ttggcgagct ggttaccgag
     6421 tggctggatc cttcactatt gcaaggcgaa gtggtgtcag acggtccgtt aagcccggcc
     6481 gaaatgacta tggaagtggc gcagctgctg cgcgatgctg gcccgtgggg gcagatgttc
     6541 ccggagccgc tgtttgacgg tcatttccgt ctgctgcaac agcggctggt gggcgagcgt
     6601 catttgaaag tcatggtcga gccggtcggc ggcggtccgc tgctggatgg tattgctttt
     6661 aatgtcgata ccgccctctg gccggataac ggcgtgcgcg aagtgcaact gacttacaag
     6721 ctcgatatca acgagtttcg cggcaaccgc agcctgcaaa ttatcatcga caatatctgg
     6781 ccaatttagc gtcatcttct ctataaaaaa gagcgtggat tgggtacaat cccgctctta
     6841 tcaccgcatt ttgactagct caataaaaga aatcagacca tgtttgaaat taatccggta
     6901 aataatcgca ttcaggacct cacggaacgc tccgacgttc ttagggggta tctttgacta
     6961 cgacgccaag aaagagcgtc tggaagaagt aaacgccgag ctggaacagc cggatgtctg
     7021 gaacgaaccc gaacgcgcac aggccctggg taaagagcgt tcttccctcg aagccgttgt
     7081 cgacaccctc gaccaaatga aacaggggct ggaagatgtt tctggtctgc tggaactggc
     7141 tgtagaagct gacgacgaag aaacctttaa cgaagccgtt gctgaactcg acgctctgga
     7201 agaaaaactg gcgcagcttg agttccgccg tatgttctct ggcgaatatg acagcgccga
     7261 ctgctacctc gatattcagg cggggtctgg cggtacggaa gcacaggact gggcgagcat
     7321 gcttgagcgt atgtatctgc gctgggcaga atcgcgtggt ttcaaaactg aaatcatcga
     7381 agagtcggaa ggtgaagtgg cgggtattaa atccgtgacg atcaaaatct ccggcgatta
     7441 cgcttacggc tggctgcgta cagaaactgg cgttcaccgc ctggtgcgta agagcccgtt
     7501 tgactccggc ggtcgtcgcc acacgtcgtt cagctccgcg tttgtttacc cggaagttga
     7561 tgacgatatc gatatcgaaa tcaatccggc ggatctgcgc atcgacgttt atcgcgcgtc
     7621 cggcgcgggc ggtcagcacg ttaaccgtac cgaatctgcg gtgcgtatta ctcacatccc
     7681 aaccgggatc gtgacccagt gccagaacga ccgttcccag cacaagaaca aagaccaggc
     7741 catgaagcag atgaaagcga agctttatga actggagatg cagaagaaaa atgccgagaa
     7801 gcaggcgatg gaagacaata aatccgacat cggctggggc agccagattc gttcttatgt
     7861 ccttgatgac tcccgcatta aagatctgcg caccggggta gaaacccgca acacgcaggc
     7921 cgtgctggac ggcagcctgg atcaatttat cgaagcaagt ttgaaagcag ggttatgagg
     7981 aaccaacatg tctgaacaac acgcacaggg cgctgacgcg gtagtcgatc ttaacaatga
     8041 actgaaaacg cgtcgtgaga agctggcgaa cctgcgcgag caggggattg ccttcccgaa
     8101 cgatttccgt cgcgatcata cctctgacca attgcacgca gaattcgacg gtaaagagaa
     8161 cgaagaactg gaagcgctga acatcgaagt tgccgttgct ggccgcatga tgacccgtcg
     8221 tattatgggt aaagcgtctt tcgttaccct gcaggatgtg ggtggccgca ttcagctgta
     8281 cgttgcccgt gacgatctgc cggaaggcgt ttacaacgag cagttcaaaa aatgggacct
     8341 cggcgatatc ctcggcgcga aaggtaaact gttcaaaacc aaaaccggcg aactgtctat
     8401 ccactgtacc gagctgcgtc tgctgaccaa agcactgcgt ccgctgccgg ataaattcca
     8461 cggcttgcag gatcaggaag cgcgctatcg tcagcgttat ctggatctca tctctaacga
     8521 tgaatcccgc aacaccttta aagtgcgctc gcagatcctc tctggtattc gccagttcat
     8581 ggtgaaccgc ggctttatgg aagttgaaac gccgatgatg caggtgatcc ctggcggtgc
     8641 cgctgcgcgt ccgttcatca cccaccataa tgcgctggat ctcgacatgt acctgcgtat
     8701 cgcgccggaa ctgtacctca agcgtctggt ggtcggtggc ttcgagcgtg tattcgaaat
     8761 caaccgtaac ttccgtaacg aaggtatttc cgtacgtcat aacccagagt tcaccatgat
     8821 ggaactctat atggcttacg cagattacaa agatctgatc gagctgaccg aatcgctgtt
     8881 ccgtactctg gcacaggata ttctcggtaa gacggaagtg acctacggcg acgtaacgct
     8941 ggacttcggt aaaccgttcg aaaaactgac catgcgtgaa gcgatcaaga aatatcgccc
     9001 ggaaaccgac atggcggatc tggacaactt cgactctgcg aaagcgattg ctgaatctat
     9061 cggcatccac gttgagaaga gctggggtct gggccgtatc gttaccgaga tcttcgaaga
     9121 agtggcagaa gcacatctga tccagccgac cttcattact gaatatccgg cagaagtttc
     9181 tcctctggcg cgtcgtaacg acgttaaccc ggaaatcaca gaccgctttg agttcttcat
     9241 tggtgggcgt gaaatcggta acggctttag cgagctgaat gacgcggaag atcaggcgca
     9301 gcgcttcctg gatcaggttg ccgcgaaaga tgcaggtgac gacgaagcga tgttctacga
     9361 cgaagattac gtcaccgcac tggaacacgg cttaccgccg acagcaggtc tgggaattgg
     9421 tatcgaccgt atggtaatgc tgttcaccaa cagtcatacc atccgcgacg ttattctgtt
     9481 cccggcgatg cgtccggtga aataagcatt acgttatgct cacaaccccg gcaaatgtcg
     9541 gggtttttta attgtgctgc gcgaaagcag acaacaattt tcttgcttca ctattggctg
     9601 cctgcatcac catccacgga ctgaacgccc acggcgtggc atcaataccg tgtaatacat
     9661 ctgctaaatc acaccattga taatccatca cttcatcatc gttgatctgt aacgcactgg
     9721 tggtgcgtgc ggcaaatacc ggacacactt cattttccac aatgccattc ggatcggtgg
     9781 cgcgatagta gaagtcagga tagatagatt caggaggcgt aatttccacg ccaagctcat
     9841 aacggcaacg gcggatcacc gcgtcttcgt tgctttctcc cagttgtggg tgcccacaaa
     9901 ccgagttagt ccacacgcca ggccacgctt ttttgctaag ggcgcggcgg gtaactaata
     9961 attgcccttt ggcattaaac agccaactgg agaacgcgag atgtaagaga gtgtctgccg
    10021 tgtgtgcggc atacttttcc agcgtacccg tgggaactcc ctgtgcattc aataaaatga
    10081 cgtgttccgt ttgcataatt tctcacatgt aattctaatc actcatcatt atagataatt
    10141 gaagagtgaa atgattaacc aagtgagcgt ttatcgacaa ccgcccgttt tgagcggatg
    10201 tcgacaggta aaaaccattt aaaaaagtag catcatatat ttgaagcgac tttcaatggc
    10261 cattgcaaca cagtcagaaa tgacaggaat agagagagta aaaagcaaca gcagactgaa
    10321 tatagcgcgc attaaaagat tcatattatt ctccatgctc atttttcttc agcttgcgca
    10381 tcagaagcca catacgtact gtcctgactt ttaaatcttt atcagagacg gattccttca
    10441 ccagatcagc atgatgctga tagccattaa agaagacgtt cagcacaacg gcgctaaggg
    10501 ttgcgagcat aatgccgcta tgcagcaacg gttgcagtac ggccggtaac ttagaaaaga
    10561 aatcgtgaga gagcgtcgga gtcatgccaa cgccgagact gatcgccaca atatagaggt
    10621 tgtaacggtt ggtggtgtag ttacagcgcg acagaattcg aatccctgtc gccagtacca
    10681 tgccgaacat caccagacca gcgccgccca gcacaaattg cggaatggag gcgaccagca
    10741 ccgccatttt tggcaccatg ccaaacagga ttaatataat tcccgaagaa atacacaccc
    10801 agcggctatg aacgcgcgtc acgctaacca ggccaacgtt ttgagaaaat gacgtatggg
    10861 ggaagctgtt aaacgttccg cctatcattg tccctacgcc atcgacacgc agcccgcgaa
    10921 taatatcgtg tgaagagagt ttacgaccaa ctatttcacc cagtgccagg aacatcccca
    10981 tcgactcgat aaacacgatg attaacacgg cagtcatggt cagaatggaa acgggatcaa
    11041 aaatcggcat accaaacgac atcggggtga caatcgcaaa ccacgaggca tcatgtagcc
    11101 cggataaatt gacttcattc atcatccacg aaagtaaaaa gccaaataca atccccagta
    11161 atacggcgac gttggacata aatcctttcg catagcgagt aatgagcaag ataaaaatta
    11221 agacggcaaa ggagatacct aaataaacgg gattaccata ttgcggattc cctttacctc
    11281 ctgcggccca gtcaataccc acctgaatga tgctaagtcc gatagaagta ataaccacac
    11341 cggtaaccag tggcgggaat aaaggcatca agcgaccgat aagtggcgct aataatgtgg
    11401 tgataaaacc cgcggcgata gtggcaccaa atatccccag caggccgata tccgggttca
    11461 tcccaatggc tatcattggt gttacagcgg cgaaggtcac cgacataatc accggcaggc
    11521 ggatccccat aaagcggccg ataccgatac attgcaataa tgtgacgatc ccgcagcaaa
    11581 agagatccga gctaatgagc atcgcaatag cttcttttga gaggcccagt cggtcgccaa
    11641 tcataagagg aacagcgact gcacctgcgt acatgacaag gacgtgttgc agaccgagta
    11701 tgatcagctt tcctggtgat aatatgcggt caacctcatc agttgggcgg tcttgcccag
    11761 aagatgaggg aagttgggaa tctatggcgc tcatcagtgt ttctcctttc gcccgccaga
    11821 gtaaaaagca accgtttacg ttcatgaaac tgtctaaata catgctcgtg gagtgccagt
    11881 tttgcgagga aggggagtta gagacaggaa ttgcggaggt aatcacataa tcgtggttaa
    11941 accacgaaaa tgagaagaga acctgacaat ggaattatct gtaattttct catattgata
    12001 tttcattaat cattctggca tgtcgcactc tcgcatttaa tcgtttttat ctggatagca
    12061 ttcttttgat cggcaatgtt gaaataatga aagctgcata gattgagggg caataatttg
    12121 aactcagtcg caaaattata attaaatcgg ccatgctacc taatacatgc ccatttattg
    12181 catcgcgaaa tttgagaaga cataactcat taaatgaaag ggatgcaaat gaataagttt
    12241 atcgctgctg aagctgcgga atgtataggc tgtcatgctt gtgaaattgc ctgtgcgatg
    12301 gcacataatc aagaaaactg gccgctgagt cacagtgact ttcgaccgcg tatccacgtt
    12361 gtagggaaag gccaggctgc gaatccggtg gcctgccatc actgcaacaa tgccccttgc
    12421 gttacggctt gtccggttaa tgctctgact ttccagcccg atagcgtaca actggacgag
    12481 caaaaatgta ttggttgtaa aagatgcgca atcgcttgcc cctttggcgt cgttgagatg
    12541 gtcgatacga ttgcgcagaa atgcgacctt tgtaaccagc gcagttccgg cacgcaagcc
    12601 tgtattgaag tctgcccaac acaggcgtta cggctgatgg acgataaagg gttgcagcag
    12661 ataaaggtgg cccgccagcg caaaacggca gcaggaaaag cgtcatcaga cgctcagcca
    12721 tctcgcagtg cagcgttgct ccccgttaac tcgcgtaaag gcgcagataa aatttcagcg
    12781 agtgaacgga aaacccactt tggcgaaatc tattacgggc tggatccaca acaagcgact
    12841 tatgagagtg accgctgtgt ttattgcgcc gaaaaagcta actgcaactg gcattgcccg
    12901 ctgcataacg ctattccgga ttacatccgt ctggtgcagg aaggaaagat tattgaagcg
    12961 gcagaacttt gccaccagac cagttccttg ccagaaatct gcggcagggt atgtccacag
    13021 gaccgtcttt gtgaaggcgc atgtactttg aaagatcact ctggcgcagt ctctatcggg
    13081 aatctggaac gctacattac cgataccgcg ctggcgatgg gctggcgtcc cgatgtcagc
    13141 aaagttgttc cccgtatcga aaaagtggcg gtgattggcg cagggcctgc agggttaggg
    13201 tgtgctgata ttttggcgcg cgcaggagtt caggttgatg tctttgatcg ccatccagaa
    13261 attggcggta tgctgacttt tggcatacct cctttcaaac tcgataaaac ggtattaagc
    13321 cagcggcgag agatattcac cgcaatggga atcgactttc atcttaactg tgaaattggc
    13381 cgcgatatta cctttagcga tttaacttct gaatatgatg cagtttttat cggcgtgggg
    13441 acttacggga tgatgcgagc agatctgccg catgaagatg cgcccggtgt cattcaggcg
    13501 ttaccgttcc tgactgccca tacccgccag ctcatggggt tgccggagtc tgaagagtat
    13561 ccgctgacgg atgtggaagg taagcgagtc gtggtattgg gcggtggcga tacgaccatg
    13621 gattgtttgc ggacttccat ccgcctcaat gccgccagcg tgacctgcgc gtatcgtcgt
    13681 gatgaagtca gtatgccggg ctcgcgcaaa gaggtggtca atgcgcgcga ggaaggtgtc
    13741 gagtttcagt tcaatgttca gccgcaatat atcgcttgtg acgaagatgg gcgtttaact
    13801 gcggtgggcc tgattcgtac cgccatgggt gagccggggc cggatggtcg ccgtcgtcct
    13861 cgtccggtag cgggttcaga gtttgaattg cccgccgatg ttctcattat ggcctttggt
    13921 ttccaggcgc atgccatgcc gtggttgcag ggcagcggaa ttaaactcga taaatggggc
    13981 ctgattcaaa ccggtgacgt cgggtattta cctacccaga cgcatctgaa aaaagtcttt
    14041 gctggtggtg atgctgttca tggcgcggat ctggttgtca ctgcaatggc cgcaggaagg
    14101 caggcggcgc gcgatatgtt aactctgttt gatacgaagg catcatgatg aaatcgttaa
    14161 ttatcgttaa tccggctgac tgtattggct gccgtacttg tgaagtagcc tgtgtggtcg
    14221 ctcacccttc agaacaggag ttgaatgccg atgtcttttt accccggctg aaggtacagc
    14281 gactggacag catcagtgcg ccggtgatgt gccatcagtg tgaaaacgcc ccttgtgtta
    14341 gcgcttgccc cgtgggggcg ctgacgatgg gcgagcaggt ggtgcagacc aattctgccc
    14401 gttgtattgg ctgtcagagt tgcgtcagtg cgtgcccgtt tggaatgatc accattcagt
    14461 cgttgccggg ggatacccgg caacaaatcg tgaaatgcga tctctgtgaa cagcgggaag
    14521 aggggccagc ctgcgttgaa tcttgcccaa cacaggcgct gcagttgctt actgaaagag
    14581 aactcaggcg agtccgccag cagcgtattg ttgccagcgg tgagaatcct ctgtgaaaga
    14641 aatctccgcg ctgaaaggcg cggaggatct attagattgc ccaaccaccc gcgtaaaatg
    14701 cgaccagtgc ggcagtaatg atcaccgtac caatattcag cttttgccat tcgcgtgcaa
    14761 agacgcggcc tacgaccagt gtcacaaagc caagcataat accggtaacg atattacaag
    14821 tcagaacgat gaacacggca cacaccaggc cagccattgc gtcaataaaa tcattgaaat
    14881 ccagcttcga gacgttactt aatatcagca aacctacgta catcagagcg ggggcagtgg
    14941 cgtaaccagg gatcaaaaat gacagcggtg ataagaacag aatcagcagg aataacgccc
    15001 ccactacggt tgcggttaaa ccggttttcc ccccggcggc ggttcctgcc gctgattcga
    15061 tataaaccgc cgcgggcgct gcgcccacca ggccggagaa tattgaactt actgagtcac
    15121 tggtcagggc tttgccgccg ttgatgatct ggttgtcttt atccaacaaa ttcgcctgac
    15181 cggcgacggc acggatggtg ccagtggcgt cgaacactgc ggtcatcacc aatgccagta
    15241 cactcggaag tacagttggc tggagtgcgc ccataatatc gaggctgaaa atcagagact
    15301 taccatcttc gccagtcagg cttggcatcg ccaccagacc gtggtacttc accgctgggt
    15361 caaagattaa gccgatgatc gaaattgcaa taatcaccaa caagatcccg ccgggtacgc
    15421 gacacttctc cagaccgaag ataaccgcca gccccagcaa gctcatcatc accgggaagg
    15481 aggtaaacgc accgagcgcc actggcaagc cttcaatcgg gtttttgata accattccca
    15541 caccgttagc agcaatcagc agcagaaaca ggccgatacc gatacctgta ccgtgagcaa
    15601 tccccatcgg caaattacgt aagatccagg tacgcacacc ggttacggaa atggcggtga
    15661 agatgacgcc catcagaaat accgcgccca gtgcgacagg aacgctaatt tgttgcccga
    15721 gtaccagact gaatgcggta aacgccgtca aggaaatcgc acaaccaatc gccattggca
    15781 aattggccca caaccccatc agcaacgagc cgaagcccgc gaccagacag gtggcaacaa
    15841 acacagctgc gggaggaaaa cctgctttgc ccagcattcc cggaacgacg ataacggaat
    15901 aaaccatggc cagaaaggtc gttaagccag ccagtacttc ctgacgaacg gtactgccac
    15961 gagcggtaat tttaaaataa ttatccagcg cgccctgtgg ctttggtgcg tccggtgttt
    16021 gtaggatgtc tccagacata atgatgtcct cacgaggatg ttaaagtttt attattagtt
    16081 gcgttcgtac accagacgac catcaacgta ggtgcggtag atcgaacggt catcgcccaa
    16141 cgtcatcatc acgaacaatt tgtcgactaa agaaacagag ttgtcatagc gcagctgctg
    16201 tagcggagtg gcagtgggtt ccatcaccac gaaatcagcc tctttgccag gtaaaaagtt
    16261 gccaatcaaa tcgtcaaggc ccagagattt cgctccgccg agcgtggcca ggtaaaacgc
    16321 ttcatatgcc gagaggcgat agccttgtaa ttgcaatacc ttgtaggctt cgttcagcgt
    16381 ttgcagcatg ttaaaagttg ttccggcacc gatatccgtt cccatgccca ctttgacttt
    16441 cttctgccat gcttttttca agttgaataa gccgctgccg aggtaaaggt tggaggtcgg
    16501 acagaaagca atgctggatt tggtttcgct gagacgatcc cactcttttt cttcgagatg
    16561 gacgcagtga gcaaagacac agtttttacc ggtcagaccg tactgatggt aaacatccag
    16621 ataaccatca tggtcaggat aaagcgattt gacccaggca atttcatctt tgttttcaca
    16681 gagatgggta tgtacccacg tatccggata ttcttctttc aggcgttgcg ccatcgccat
    16741 ctgttcagga gatgaggtag gggcgaagcg tggcgtaatc gcatacaaca gacgaccatt
    16801 tttgtgccag cgttcaatca gttctttgct ttggtgatag ctgctttcgg cagtgtcgag
    16861 cagataatcc ggtgcgtttc ggtccatcat caccttaccg gcaatcatac gcatattgat
    16921 atgactggcg gcttcaaaca gcgcatcaac ggattgtgga tgaacagtgc caaacaccag
    16981 cgccgtggtg gttccgttac gtaaaagctg cttgatgaag aacgccgaca tctcgcgggc
    17041 gtactctaaa tcctcataac gacgttcagt agggaaggtg tgtttattca accactccag
    17101 caattgttca ccataggccc ccaccatttc actttgcgga taatggatat gtgtatcgac
    17161 aaagcccggt actatcagtt tgccgcgata gtcgcgcacg cgaatggtgt caggaatttg
    17221 atgctttccg tcttcccatt cgccaaacca ttccactttt ccctgtttaa tgagtaataa
    17281 accatcctca ataaaccgca gcgcagaggc aatttcttcc gggttatcga ccgtacgggt
    17341 gacatcaata aaactgcctc gtaccgcttt taacgtgtgt tctcctgaca tcattgactc
    17401 ctttactgtt aatccatctc ttccgctgag gtaataccag gcagaacgtt ttcctgtcgg
    17461 tagccaccag ggaggataat atttaataaa atcgcagtta acccgccagc acaaataggg
    17521 ttttcaacta atacataaat agaggctggc aatattttaa aaatttcggg atcgtaggag
    17581 acgccaagcc ctaaacctaa agacgtggcg acaataagtg tttctcggcg ctttaatccg
    17641 ttggtgatga tgatgcgaat cccagcgatg gcaatcatgg aaaacatcaa cgtcattgcg
    17701 cctcccagaa ctgccgaggg aatggtcgtg aagaagccgc caatcatcgg aaataagccg
    17761 aggataacca gcattaccgc gatggttcgc ccgacataac gtgaagcgac gccagtcatc
    17821 tgaataaccc cattattttg cgcaaacgtg gttaatggta atgaaccgac agcggaggcg
    17881 ataacagaaa ccagaccatc tgccagcacg ccgcctttca gtcgggactg atactcttcc
    17941 ccctgaatgg ggcggcggga aaccattgcc gtggcggtga tatcgccgac tgcttccagc
    18001 acgctaagca gataaatcgt gccaaccacc aggaactgat ggaagctaaa actaaagccg
    18061 tatttgaacg gatgcgggat ggtgattaac ggcaaattgc gcatactgct gaaatccacc
    18121 atgcccaggc ataacgatgc gatatagccg acacatagcc caatggcgat ccctcccatg
    18181 cgtagcaacg gactgcgaca gcagttaaag ccgatcacca caattaaaac caataaacca
    18241 acgccgagat gttcgtaatt gccgaacgta ccgctgcttt tggctgcaaa tcctccacca
    18301 aaatcgataa tgccgacttt aatcaggctt aagccgatca tcagtaccac aataccgctg
    18361 acggtaggcg taataacccg acgtaaatag ggcaggataa aagaagagcc gacaaccaga
    18421 aatgcgccaa cgaaggagac gccgaggagc gacgacatga ttaactcttc gtgaaaaccg
    18481 tcgcttttca tgctgctgcc cagcgcaatc atgaccgtaa caaatgaaaa attgactgac
    18541 tgaattgaga gaagaccaga accgacgatg ccgtagcggt ttacttgtaa ccaggtacca
    18601 ataccagagg cgatcatcgc catagaaaca agataggcag ttgtttcagc ggaaagctgt
    18661 aaggtcgcac ccacgattaa cgcgggggtt accatcggaa cgaaaattgc caacagatgg
    18721 gtaatggcac ctacgagagc ctgatgaaag ggagggcgat cctccagttc aaatataagg
    18781 tcagaacctg catggtttat atcagacatc ccttcactcc ttttaaagta aatttgatca
    18841 tctgaattag taaaataaat tcccacgaat ttggggcgca ggacgccttg agaacggctt
    18901 atctcatttt cgcagtcact atttagtcat cgttcaaac
//
LOCUS       DAZGAP010000102        18930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGAP010000102 DAZGAP010000000
VERSION     DAZGAP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.102"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..660
                     /locus_tag="ACHLAM_003072"
     CDS             <1..660
                     /locus_tag="ACHLAM_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO7854564.1"
                     /translation="VREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEV
                     KSPALREMVVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFITESIAQAVESRT
                     GAPPARLLAMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLM
                     YIVENQDGSVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDI
                     D"
     gene            complement(662..741)
                     /locus_tag="ACHLAM_003073"
     tRNA            complement(662..741)
                     /locus_tag="ACHLAM_003073"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            782..1255
                     /locus_tag="ACHLAM_003074"
     CDS             782..1255
                     /locus_tag="ACHLAM_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO7854565.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLTRSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            1432..2556
                     /locus_tag="ACHLAM_003075"
     CDS             1432..2556
                     /locus_tag="ACHLAM_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO7854566.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            2556..3503
                     /locus_tag="ACHLAM_003076"
     CDS             2556..3503
                     /locus_tag="ACHLAM_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO7854567.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            3547..3933
                     /locus_tag="ACHLAM_003077"
     CDS             3547..3933
                     /locus_tag="ACHLAM_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO7854568.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEAEAGQDAPWKKTGNKAKQARA"
     gene            3930..4349
                     /locus_tag="ACHLAM_003078"
     CDS             3930..4349
                     /locus_tag="ACHLAM_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO7854569.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            4346..4906
                     /locus_tag="ACHLAM_003079"
     CDS             4346..4906
                     /locus_tag="ACHLAM_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO7854570.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            4907..5152
                     /locus_tag="ACHLAM_003080"
     CDS             4907..5152
                     /locus_tag="ACHLAM_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO7854571.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            5149..6648
                     /locus_tag="ACHLAM_003081"
     CDS             5149..6648
                     /locus_tag="ACHLAM_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO7854572.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            6657..7022
                     /locus_tag="ACHLAM_003082"
     CDS             6657..7022
                     /locus_tag="ACHLAM_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO7854573.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            7037..7513
                     /locus_tag="ACHLAM_003083"
     CDS             7037..7513
                     /locus_tag="ACHLAM_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO7854574.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            7640..9715
                     /locus_tag="ACHLAM_003084"
     CDS             7640..9715
                     /locus_tag="ACHLAM_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO7854575.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            9702..11051
                     /locus_tag="ACHLAM_003085"
     CDS             9702..11051
                     /locus_tag="ACHLAM_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO7854576.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            11035..12159
                     /locus_tag="ACHLAM_003086"
     CDS             11035..12159
                     /locus_tag="ACHLAM_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO7854577.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRALEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSGR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGEVDAFVRAQMKKQGIKFNDE"
     gene            12149..12763
                     /locus_tag="ACHLAM_003087"
     CDS             12149..12763
                     /locus_tag="ACHLAM_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO7854578.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            12756..13193
                     /locus_tag="ACHLAM_003088"
     CDS             12756..13193
                     /locus_tag="ACHLAM_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO7854579.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSTVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            13193..14275
                     /locus_tag="ACHLAM_003089"
     CDS             13193..14275
                     /locus_tag="ACHLAM_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7854580.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            14266..14826
                     /locus_tag="ACHLAM_003090"
     CDS             14266..14826
                     /locus_tag="ACHLAM_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO7854581.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHKEENI"
     gene            14826..15737
                     /locus_tag="ACHLAM_003091"
     CDS             14826..15737
                     /locus_tag="ACHLAM_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7854582.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(15772..16293)
                     /locus_tag="ACHLAM_003092"
     CDS             complement(15772..16293)
                     /locus_tag="ACHLAM_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7854583.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            16650..16835
                     /locus_tag="ACHLAM_003093"
     CDS             16650..16835
                     /locus_tag="ACHLAM_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854584.1"
                     /translation="MFISCVPSKTTPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGFSTSF"
     gene            16849..17409
                     /locus_tag="ACHLAM_003094"
                     /pseudo
     CDS             16849..17409
                     /locus_tag="ACHLAM_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            17509..>18930
                     /locus_tag="ACHLAM_003095"
     CDS             17509..>18930
                     /locus_tag="ACHLAM_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7854585.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLITDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSAWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNL"
BASE COUNT         4345 a         4841 c         5571 g         4173 t
ORIGIN      
        1 gtcagagaaa tgccggtgac cacatattca gatggcacca gaaccatgac accggatgtg
       61 ggctggtcat ataaccccgg ttcggcggcg ttcggcacag accaggcgct gatccgtaaa
      121 ctgatcgagg taaaaagccc ggcgttacgg gaaatggtgg ttcaggagat gaacaacagc
      181 ccggagcggc agctggcgtt ccgtatctgg gccaaaaaca tcatgaagac ccggcgaggc
      241 ggtaacgata tccgcacgct gggctttata accgaaagca ttgcgcaggc cgtggaaagc
      301 cggacgggag cgccaccggc ccgcctgctg gcgatgagcg gtaaaaatgt gctccatgcg
      361 gacagcatga aacatcagaa tgacggcatt gccctgacgc cggaggattt cgggcgatta
      421 ccggcaatgc tggcaaagcc aaaagcggta ctgtgggata aacgccacaa caatctgatg
      481 tatatcgtgg aaaatcagga tggcagtgtg cagattgccg ttaatgcgcc gtacagcctg
      541 aagcgtcagc cggataaact ggatgtgatt gtgaatgctt accgggttat caatatggat
      601 aagctgaaat cggatatccg gggcggaatg cttgaggtac tggaaggaga tattgattaa
      661 cggtcagtgg cgggggtcga acccgcatac atgatccacc cggaggcagg caccgacttt
      721 accagttaag cgtacactga ccgcctgctt attttatatt catattttat acggaggcaa
      781 tatgtcatcc attgatgccg ccgtcgttgt tgacgttacg cgtctccagc gggtctttgc
      841 ccggcttcag tttgtgggtg gtggaaaaga cctgacccgc agtgtggcgt ccagccttct
      901 gtcatcgtca gaaatggcgt ttgaacagga aaaagagccg gacggcgaac gctggcatga
      961 ctggtcagat ccttaccgca agtggcgtac ccgtaaggga tacatgcccg gcaaaatcct
     1021 gacgctgaac ggcgatctgg cccgacgcct gaccacggat tatggcgata cctgggcgct
     1081 gattggatca aatgagcctt atgcggccat tcatcagtgg gggggcctgc caggtatgcc
     1141 gcctggaccg gcggccattg gtgcacgtcc gtatatgggc tttgatcagg tggcagagca
     1201 ggagatcatg gacgaaatca gaaaacgctt taaaaaggcc acagaaacgc cttaattgtt
     1261 taaggtatgc aaatgcatta ccttaccccc ttcaggcgcg tgtcgtgatt ttctaacctg
     1321 aatttaacgg gctgtaaaat ctgcgtgaag cgcctttgta tttttcccct gaaagcactt
     1381 cctgaaatcc cgcaaaaccc gccctgaaaa ttcccggcct atgctgccgg aatgaagacg
     1441 aaaaacacgc ccaaacttgc ttatgccatt ctgaatgcca tcagcctgtc cgcagacggg
     1501 gacggtgact ggtgtcagat catgccagcc ggtcgggtaa aggcccgtga cggtcgcccg
     1561 gaaaaaccgg ccgagggctg gctgatcaac catgcagccg ttgagcgcat ggtctcccgt
     1621 gttgtggcgc tcaatcagcc ggtgaaaatt gactacaacc accagaccct gattgaggga
     1681 catccggcac cggctgccgg ttttgtgatg gcctcaccgg agaattttcg cttcagtgaa
     1741 gagcgaggtt tcgaggtgcg cccgaagtgg aaccctccgg cccttgaaca tctgcgtaat
     1801 aacgaattcc cctggttttc accggtgatt ggctatgacg agagcaccgg cgaacctgtc
     1861 gaacttcgga tgctggctat taccggtgac cccggtctga ccggcatgaa tcctgtcgcc
     1921 gcactgtcgg cggatgacct ttataacgcc ttaaaccctc ctttaaagga tacctccatg
     1981 aatgagcaat tacgccagtt gctgacggcg ctcggtctga ccgtggctga cggtgacgaa
     2041 tttacgccgg agctgggcac ggcagcgctg tctgccctca ccgggatcaa aacccgtgcg
     2101 gatgcacacg acaacctgaa aacacaggtc gccagtctgt cggcagagct ggaaaccgca
     2161 aaaggcacac cggctggcgg cagtattgat ctgacgaaat acgtgcccgt tgaaacgtat
     2221 aacgccctgc gtaccgaata cgttgcgctg tcggcacagc acggcagcac cacactggag
     2281 caggtgctgg acaaggctga atccgaaggg cgcatcttca aaagcgaacg cggctatctg
     2341 gaacaactgg gtgggcagat tggtgttgcg gcactttctg cacagcttga cgcccgacag
     2401 ccggttgcgg ccctgaccgg ccttcagacc gacaccgtga ccgtgccgga taaaaagact
     2461 gccaccgctg cgctgtcggc tgaagatatc gccgctgccc atctgctggg taaaaccgaa
     2521 gccgagttcc tgaaaatgaa agaggaaatg caataatgcc taccccgatt acaccggcga
     2581 tgatcaccgc cctgatgacg ggctaccgtt ctgattttca ggccgggatg tccatggccc
     2641 cgtcgcagta caaaaaaatt gcgatgaccg tgccctccac ctcgaaatcc aacacctacg
     2701 gctggctggg gcagttcccg cagttccgtg agtggatcgg ctcccgcgtc atcgagaaga
     2761 tgaaagccta tggctatgcc atcgtgaaca aaacttttga aggcactgtc gccattagcc
     2821 gcgatgactt cgaagacgac aacctcggta tttattcccc gttgttccag gagatggggc
     2881 gcgctgcggc ggtacaaccg gatgagctgg tctttgctgc cctgcgtgac gggatcagtg
     2941 cggcctgcta tgacggtcag aacttctttg ataccgaaca tccggtttac cccaaagtgg
     3001 atggctccgg ggatgcgcag atggtcagca atatgtttgt ggcaaaaacc ggctctgtcg
     3061 gcgcacaggc tgattacagc ggtccggcct ggtatctgct tgactgttca cgcgcggtaa
     3121 aaccgctgat ttatcaggat cgccgtaagg ctgaacttgt tgcccagacc aaagtcgatg
     3181 aaggtcgcgc attcaccgat aacgaatttg tgtttggtgc atccgcccgt cgcaatgtgg
     3241 gctacggctt ctggcagatg gcctacatga tgcagtcacc gctgacgctg gatgcactgt
     3301 ggcacggctg gtcagccatg cgcgaattta ccgctgacgg tggccgcaaa ctcggcatta
     3361 aacccaccca tattgttgtc cccacctcgc tggaaaaaca ggcggtgcag ctgctggagc
     3421 gtgaactgtt cgcagacgga aacgcaaccg tctctaacga gatgaagggc aagctggagc
     3481 tggttgtcgc ggattatctg taaacggtga gccgggcttg cggcccggcc cctgcggagg
     3541 gcaaatatga atgaacatca cactgtggca gcaacagatg attcaggtct tcaggtcagt
     3601 ggtgacaatt ctgtcacggt gctggcagaa gtgcgttgca gtcgttcggc gtttcgtcgt
     3661 gcggggtttc tgttcacgcg tggacgtcag caggttgagg tcaccccgga gcagcttgcc
     3721 cgactggagg cggagccatg cctcactgtg cgaatactac agacgtctgc tgatgatgcg
     3781 gggagcgtgg cgggtgtggt tcatgcagtg gccggtacag atttagccga agccgaagcc
     3841 gaagccgaag ccgaagccga agccgaagcc gaagccggac aagatgctcc ctggaaaaag
     3901 accggtaaca aagcgaaaca ggcccgtgca tgaattacgc cactgaaacc gatatgcggg
     3961 cgcgttaccg cgaggatttg ctcaggccgt tactggctgt gccccgttcg gatgagccgg
     4021 atacgcgcaa gctgaaccgg gcgctgacag atgcgtcagc ccttatcgac agctatctgt
     4081 ccgcccgtta cacgctgccg ctggaggtta ttccggccgt tcttgttcag cactgttgcg
     4141 cgattgcctt ttattacctg tgcgatcagc gagcctcaga tcaggcgcgt gaccgttacc
     4201 gtgaggcgct ggcctggctg aaagatgtca tgaacggcaa tgtgccggta ggtgtggata
     4261 cgaacggagc ggcccctgaa tccggggatt taccacaggt tcagtctgat gcagcggtat
     4321 tcgggcgcaa ccagaagggc ttcatatgat tacggaaacc gaacaggcat acatcgcccg
     4381 tatccgtgag tatttcggga atgaactggt gtctgttgac acgcatcccg gcgactggag
     4441 cgacagcgta ctgcgcacca tgctgattaa cgccccggcg atctacgttg cctggctggg
     4501 tgccggtgaa ggccgtaccc gtggtcgcct ggtcagtcac tgggtgttct acgtcatcgg
     4561 cgacatgctc aacgggcgtg aggccagccg tcccggattg tatcagattg tggcccggct
     4621 gattgccgtg cttaacggct tcagaacaga aaaaacctca ccgctttact ttgaaaaggc
     4681 ggtcaacggt tacaccgaaa cccaggcgga cagtggtgcg gtgatgtatg cgctttattt
     4741 ctcctgcgag gaaatgatcg caccgctgac cgatatcagc tcgctggacg acttcctgcg
     4801 tcattacgaa acctttgtgg agccgcccgg tacgccgccg tttgaggcac atattgattt
     4861 gccggggaac acgacaacgg cggtcgaacc gccggaggaa ctgtaaatga aaaccatctt
     4921 tatcaaaccc gcgccgggac gcctgattcg tgatcccgac acaatgcgcc cgctggcaca
     4981 ggagggggag gaaaagccct ttacgccgtt ctggtgtcgg cgtcttgatg acggcgatgt
     5041 cattcaggct gaaaaagccg ctgaagaggc tccggctgta tctgcggatg caacgactgc
     5101 gacagaaaaa ccggtcgccc aaccggcatc cgataaggag aaaccgcaat gattaatttt
     5161 gacgggatcg gtaatgaccg ccgtataccg ctgattgagg ttgagtttaa taactccatg
     5221 gctgtcaccg gcactccggc acagcgtcag tgtgtgctgc tgtttggtca ggcgagaatg
     5281 aaagagaatg ccgtggacgg tgccggtgtt ctggatgttc cggtcaggat cacccgtgcg
     5341 tctcaggcaa cagaattctg ggggcgcggc tccatgattg ccctgatggt ggcggaattt
     5401 atcgccatca atcctgatgc tgaactgtat gccattgctc aggggagcgg caccggtcag
     5461 gctcaggcat gtctcatgaa tataattggc accgcctctc aggatggtgt tctgcacatt
     5521 tatatcggcg gtcggcgtta tctgctggct gtcacgaaag ggcagaaagg taaggagctt
     5581 attgacgctc tggtctctgt tattaatgcc gatgctgatg cgcccttcag tgcttatgca
     5641 ggtcaggtag cagataacgc cagcggactc aagggaagta tgggaattaa tgcccgtttt
     5701 accggtgaat gttccgtaca tgatatccgt gttaattatc atgacggaga aaccacaccg
     5761 ggtggactga ccattgaact tgggtatccc aaaacaaaag ccactaaccc ggatatcacc
     5821 cgaagcgtgg cgggtatggg cgatcgccag tacaactatg tcgtcatgcc ctataaggat
     5881 gatgcaaacc tgaaaatcat cagtgatgag cttctgaaac gctggggacc ggccaaaatg
     5941 tctgacggtg tgctgtggat ggcgcatacc ggcacgtttg gtgaggtgca ggcattcggg
     6001 gcaaaacgca atgattttct ggcgacctgc acattcattc ccaaagcccc tgaaccggat
     6061 tacctctggg cagcggcggt ctgcgccacc tgtgcgccgt ctctgggggc agatcctgcg
     6121 cgtccgcttc agacgctggc gcttccgtca cgcatggccc cggccccggc cgatcgtctg
     6181 acgcgtgaag agcgtaacag tctgctgtgg ggagggattg ccacggtcag tgtggctgcc
     6241 ggtgatgtgg ttcagatcga acgtcaggtg accatgtatc gccagaacgt gtacggcgaa
     6301 agtgatccca gctatctgga tgtggaaacc atctacaccc tctcctggct gcgttattcc
     6361 ctgcgtactt ttatcacaca acgttttccg cgccataaac tggctgatga cgggacgccc
     6421 gttcgtgccg gtcagaatat cgtgacgccg gagatcatga agcttcagtt aatttcgctg
     6481 ggtgaagagt gggtggaact cgggctggtc gaaaatctgg acacctttaa aaagaatctg
     6541 cttgtggagc gtaatgcgtc agatcgaaac cgtcttgacg tgttatgcac gccggatctg
     6601 gttaaccagt tccgcttcct ggcagcacag atccgtttca ttttgtgagg taaggcatga
     6661 gcggaaaaca gtatcagggc acggccacta tccgtgtgaa cggtcaggaa tacgagacgc
     6721 ttgagggggc tacattttct ccgtcaggtt ttgaacgtga agtggtgaaa ggcgcgaaag
     6781 tctatggtta ccgccagaaa ccacgtgaag cgacactgga ctgcaaattt ccggcaggcg
     6841 gagaaggttc accagccgcc gatgaaatca acaactggac tgctgtcact atcgagtttg
     6901 tggcggatac cggcgaagtc cacatgatga cgaaggcctg gagcagtgaa ccggcctcgc
     6961 ttgacggtgg cggggagatc tccgtgaagt tcgccagcgc caccagtacc cgtgttcagt
     7021 gatcaggaaa aataaaatga ccacacgtaa gaaaaaaacg gcagtttccg aggcagccgt
     7081 gatggaggca atccgggaag cacttgaggg ggctgatcca cgcactgccg ggctgacaga
     7141 gcaactggca aagggatatg tggatctgct tgatggtctg ccgtttggtg agacccgtga
     7201 atatcgtgtc acgttccggg aactgacagc gaaagacagt attgacgcag aagcagaagc
     7261 cgagcgcgtg gtggagacaa acaatggccc gatgctgata gcgtctccgt cgttacgcgg
     7321 tgttgcactg ctgcgccgtc agattgccgc agtgggagac attgaaggtc cgttgtcacc
     7381 ccgtcagatt ggacagttaa gcgagcgcga tctctcccgc ctgatggcag cggtcagcct
     7441 gctggatacc gcgcttgccg gaaagctggc agctgaccgg gggcgatcag gcgcagtgtc
     7501 gggatcagat tgaagaagcg gcaattatcc tggggatggt gacaaaaagc ggcccggaat
     7561 gggcgcttaa cctccccctg tcgcaacttt accggcactg ccgacagaca gaaaaaatca
     7621 tcagaacgaa gcagtaagta tggcccgaaa tctcagagca tctctgatag ttgatttgct
     7681 cggcaatatc tcagccaaat cccgccagtg gtctcaggag ctgggggcat tctcacgctc
     7741 cggccaggcg gggctgggtg gtctgggaaa tgcggcccgc cgtgccgggc aggaaacaga
     7801 tgtactcgga agcaggatgc agcgcacgct ggccggggtg cggggcagta tccgccatgt
     7861 cacttctgat tttgaccgtc ttcagggcag tattaccggc accatcgggc ggatcagcaa
     7921 tctttacgga atgctggccg gtggtgcagc tgtatatggc tttaaccggg gatttattcg
     7981 cccggcagcg gagatggaaa cctatatgat tcgcctgaac tccctttaca aaggggatcg
     8041 ggcgaaaact gatgatgtcc gcaggtgggc aatacagaat gcgaaggaaa ccacctgggg
     8101 gctggctggc gtcatgcagg aatatacgtc cagtctgggc tttggtatga gtgacaggga
     8161 ggcccgaaat tttgtcacca tgcttcagga tcagggtgcc gtgggcggct ggtcactgtc
     8221 cgatgcacag ggggcatccc tgcaactgaa acagatgtat gcaagaggca gtattcaggc
     8281 agcggatgca aatattcttg ccacctacgg cattaatgcc taccgcgtgc tggctgatcg
     8341 tctgggtgtg gaccagaaag tggtcagaaa acttggtgaa aaagggctgc ttgggccgga
     8401 cagtattcgc ctgttatttc agacgctggc ggaacaggcc aggggagcac aaaaggacgc
     8461 aatgaattcc tggagtggcc tgacggccat gatgggtgac gtctgggatc agtttgcccg
     8521 ggatgtgatg gacagtgggc catttgaaaa actgaagggt aatctcaggg gatttctttc
     8581 atgggttgac agtgccaaat ctgatggcag ttaccattcc ctggcggaaa gcacggcatc
     8641 agccatgaac cagggctttg agtatgcccg tgatgcagtg acagggtttt atcaggcgat
     8701 cagaaaggta cgcgacacgc ttcaggctct gcgggatgcg ggctatggtg atgcgctgga
     8761 tcgcattggt cagggggcac agaccgccgc gaaatacctg ctgtacatgt atctcgccac
     8821 ccgtgccctg aaaatggcgc gggctgtcgg aacggggatt atacgtccgg gtgctgcatt
     8881 gctgggatac ggattatcag cggcggcctt cctgacctca cctttccggc gctctcctcc
     8941 ggccggaaca ccaccaggca catctcaggg acgagggcaa cgttttataa actttcttac
     9001 cggggtaaat ccggcagccg ttcagccagt acttgtaaca aactggccca caggaggact
     9061 gtccggcacc ggcgcgaccg atacatcctc aggcggacgt caggggcggg gccgcaggaa
     9121 acgcggaccg gggcgcggaa gaccggtcac gcctcccctg ccaccgtccc cagtgccacc
     9181 gtctccctct tctggtgggg cagggttctg ggggcgcatg atggggcgcg ccggtgggtt
     9241 actttcgtct gtcggtagcc gcatggggct ggggcgtttc tccggctttt tccgcagtgc
     9301 cggtggactg gccggccggc tgggtggagg tgccctgtgg gctggcgcga tggcagcccc
     9361 ggttctgctg gatggcagcg ccagtgccac agacaaggga gaagcggtcg gctctcttgc
     9421 aggcagtatt gccggtggtg cgctgggggc ggcagccgga cctgtgggga ttgccattgg
     9481 ttctacggtg ggcagctatc tcggtaacta tctgggggga tggctgactg aagcctggca
     9541 gaaattacgg ggcagtgatg atgaaagcag tggacaggcg gtacaaaaag cctctgcccg
     9601 tgtggagctg gttgccccgg aagggtggca ggctcgcagt attgatattg atgacacgtc
     9661 cggtcacgga ctggatgtga atgtctggaa cggagggaat tatggcctct ggtgacggtc
     9721 gtggtgcgtt ccgtggtgtg ccgttcctcg tctggcgtga gcagcgcgaa cgtggtgggc
     9781 gaaacattgt ccgtcgggaa tatccgttac gggaaaccgg tggtgcagac gatctgggac
     9841 ctaaactggc tgaatttacg ttcagtgtac ttgtgatggg ggatgatgtt cagacacagc
     9901 gaaatcgtct gcgtgatgcc cttcgtgctc ccggtgccgg ggagctgctt cacccggatt
     9961 acggcacgtt aaatgtgctg ataaacagct ttgaaagtcg ttataacgcc gctgaacagg
    10021 gcgtggttga gttcaccatt aatgtcacgc cggtaagtga tgataccgca cctgctgtca
    10081 cacaggacac ggccgccatt ctggagcaga agagtaccac cgcgctcggg caggtttttg
    10141 aaaccctgga agcgggctgg acggtgatct ctgacggaat gcacgatgtc caggcaatga
    10201 ccgaaaccat cagtgataag gtgtccgccc tggaaaatgc cgtttccggc atggggattg
    10261 tacaggatat cagtgccttt actgccacgt tcacggcgct gaaagggaat gccacggcat
    10321 tactgacagc accttcccgc atggcctcat cttttgcggg gcttttcagt gccctgatca
    10381 ccctgccatc gctgccgtca ctgtcttcag gaggcctgaa tacccggccc ggtggcagta
    10441 tcggccggac gacatccgcc gtctctcagg gaatgccgca actgtacagg acattatctt
    10501 ccctgcgtta cgtgctggat gagcaggata atccgcagac tctcatcggt ctgacaccgg
    10561 cagcacaaaa gaatattcgt ctgatacggg cggtaatgca gagtgctgcc gtggtggccc
    10621 aggcacagac cgtgggaaaa ctgctggatc aggttctcag tcaggaaacc ctgccggaca
    10681 gtgacgcagc ccaccgtacc tggcctgtct ggctggaaag ttcagttgat cttcagcgca
    10741 ttaaccgtga cttaagcgaa gcgctcgaac ggcaggtgat gacactgtcc gggcaggggt
    10801 ataccgccac ggcgctgacg cttcgtgatg ccagactggc actgacagag gatctgaata
    10861 cacggggagt tcagctaccc ggtgcaacag tagtgactgt acgtaccacc gagcctgcac
    10921 tggtgaccct gtaccgtgcc accgggaaca gtaccggctg gcaacgtttt gtgcgccgta
    10981 acggtattgt tgatccgctg ttcattcccg gaggccattc agtggaggtg attagtgagc
    11041 agcagggttg aactgtatct gggcggtgag attttttccg gctggctgac ggtgagtgtt
    11101 cgtcgctctc ttgaacatct ggcgggctcc tttgaactgg gggtaatgat gcccggtgta
    11161 cgccttccgt catccgtccg tgccggtcag tctctggaat tgcgcattga cggtcagcct
    11221 gtgatcactg gctggctgga tcaggtccgg cagcgcatca gcgccacgcg ttttcagatc
    11281 acgctcagcg gacgggataa aaccggtgac ctggtggact gttcagccat tcatccgggc
    11341 agccagtgga ggaaccgcgc gctggagcac attgcttcag atttgtgtgc tccgttcggg
    11401 gtcacggtgc gctggcaggt aaatgatgca acggcagccc ggcccttttc caccttcaca
    11461 ctggaaaatt cagaaaccgt ggcagatgcg ctgacacggg ccgcgagaca cagaggcgtc
    11521 ctggtaacca gtaatgccgc cggtgaactg gttttcactc aggccggcag tcagcgtggc
    11581 gacacgctga cgctgggcga aaatctgctg gatttggatc acaacgtgga tcaccgtctg
    11641 cgccacagtg aataccgtgt acgggggcac gggcgtggtg gtggtcatgc cggggatgca
    11701 ctgacagccg gaacgctggc cgcaccagtt ggtacggtga cagacagtgc catccaccgt
    11761 tacagaccga aaattgtgct ggcggatcat gctgttgatg cagacggtgc acgccagagg
    11821 gctgtccggg aaatgcgccg ggcggttgcc cgctctgggc gcctgacagc caccgtgcgg
    11881 cactggtttc gggagaacgg ccagttgtgg gatatcaacc tgctgacggc tgtcacggct
    11941 ccccgcaccg gagtggaaga gcgtgatctt cttgtctgtc aggtggagtt ttcgctggat
    12001 gcaaatcacg gcgaaaccac ccgtctgatt ctggcacccc gtgacggctt tattgttccg
    12061 gcagagccgg gaaacagcgg aagcggaaat gcgggtgaag tggacgcctt cgtgcgggca
    12121 cagatgaaaa aacaggggat taaattcaat gatgaatgac gaagtcatca gccgccttct
    12181 ggcccccgtg atgcggggtg ttcgtctgct gttcgggcgt ggtgtactga ccggcacaac
    12241 ggacacgctg aaaatccaga atgtgcaaat caccggtatg gatggtgaaa cctttgatga
    12301 cgttgaacgc ccccagcagt acgggcaaat cagcgtcccc ctgcctggtg cggaagtttt
    12361 tctggcctgt gctggcggac aacgggatca ggccgtggtg cttgtggtgg aagaccgccg
    12421 cagtcgcccg accggactta ccgccggaga taccggcgtg tatcaccatg aggggcaccg
    12481 tatacgtctg acaaagaacg gccggatcat tgtgacctgt aagacgctgg agatttacgc
    12541 cgatgaaggt gttcaggtgg atacaccgga ggctcacttt accggtaata tcacagtgga
    12601 taagaacctg catgtcaaag gcaatgtgtc cattgacggc accggaagat cacagggggc
    12661 gttcacgatg tccgaagcgg ttattgccgg gatcacctat tcaggtcatg tgcatcacga
    12721 taacggtgaa ggcagcaaaa cgggaggacc agagaatggc tgatattgct gtcgtctggg
    12781 atcagggttg tggttccctg caactgaacg gcgcagatct tctgacggat aacagcctgc
    12841 tgactgcggt cattatttca ctgtttacgg acaggcgggc gctggattcc gatgaaatcc
    12901 ctgacggcac ccgtgaccgt cggggatggt ggggagatag tttccgggag cgccccattg
    12961 gctcccgtct ctggctgtta agccgtgaaa agacgctgtc caccgtggtc agccgtgcac
    13021 aggcctatgc tgatgaagcg ctggcgtggc tgcataaaag cggtgctgcc acatccgtgg
    13081 tatgtcatgc catgcgtgtg gggcatgatc gcctttcgct ttccgtgaaa atcaccctgc
    13141 cggacggaag cagacatccg atgatttttt atgctgatat gaagggggaa tgatgcctta
    13201 tcagccctta ccactggcgc aactgatcac acagacacaa caggatatca gccagcgcct
    13261 gcccggttcg cagccgggcg tgaatgaaac caccctgaat gccattgcgt atgcactcgc
    13321 gggtctgtca gcacaggagc atgaacatct ggcctggatc tcccggcaga taattccgac
    13381 agaagctgat gaagccgaac tcctgaaaca ctgcgcattc tggggtgtca tccgtaaacc
    13441 ggcttcccgc gctgacggac cggtacaact gatgctgacc acggatgcag ggatcacgga
    13501 aggcgtactc cttcagcgaa gcgatggtgt tgtgtaccgc atcaccggct ctgcgaccgg
    13561 aaaagccgga acactgaatg ttaatgtgga ggcggaaagt gcggggcgcg ccggaaatac
    13621 cccgaccgga acccgcctgt cctttatcac gccacaggcg ggcatcaacc agacagccac
    13681 ggtcaccggc acgggactca ccggtggtgc ggatgtggaa acggtgccgg agttgctgtc
    13741 caggctggta ttccgggtac agaacccgcc atcaggggga acacagtatg attttgaacg
    13801 ctgggcacgg gaagtaccgg gcgtgacgcg ggcatggtgt aagcctgaat ggcctgaggc
    13861 gggtagtgtt ggtgtgactt ttgttcagga taataaccct gacattttcc ccggagaagg
    13921 tgatgtgaag cgggtggcgg attatatccg cagtcatgat gatccggcga cgggccagcc
    13981 cgttggtcag ccacttgggc cgacaatcag cgtgtttaag ctgaccaata agccggtggc
    14041 gtttgagatc aggattgtac ccaaaacgcc ggaaaatcag gctgccgtaa aacaggcatt
    14101 aacggacctg ctttataacg aatcgcggcc gggtggactt gtattgcctt catcattctg
    14161 gcgggctgtt gcaggggtga aaggactgga ggattttgaa gttcgcagtc cgctgaagtc
    14221 cgtgatggcc ggagatacag agttgctgac cgtgggggaa atcacatggc tgtaaccctg
    14281 accccgcatc agcgcgccct gttgcagttg ctgcctgacg ggctggcatg ggataagcgg
    14341 ccgtcatccg ttcttgcggc tttgtgcctg ggcctcagtc attccacgga gcgtgtttcc
    14401 tggaccggta accagatgct ggcagaacgt tttcctgatt catcccgtct gctgctggaa
    14461 gactgggagc gttatctggg gttaccggaa tgtgatatga ccggcgcaac cattcaggaa
    14521 cgtcagcgtt atgcagggaa taaataccgg atgaaaccct ctcttaaccg tgaattttat
    14581 atccggtttg cggcagagtt tggttatgaa atagatattc agccatcacc ggattcacag
    14641 tgggtcagta ttgtcacgat aaacagtgaa accggctacc ggaatatgaa tgtgctggat
    14701 gatattctca ccccgctgcg tatttatgag ggtggtgcgc tggaatgtat tctgaaccgt
    14761 tataagcctg catggcagac gtttatttac gtgtatgcaa acagtcacaa agaggagaat
    14821 atttaatgtt ccatgttgat aataattccg gcgtggcgaa tatgcctgaa ctggctccgg
    14881 cgcagagtaa taccaccacc tggtttaccg aaggtgacgg acaaaaaggt atcagctgga
    14941 ttggtcagga ctggctgaat attctccagg ccgaactgct gaatattctg gctgaagcca
    15001 gtattcagcc ggataaggcg cagttaaacc agcttacgct gtccattaaa gccattatcg
    15061 ctgcgaatgc cttttcccgg aaaaacaacc tgaaagaaat tgctgatgcc ggtgcggaag
    15121 cccagcgtct ggcccgtggt tatcttggtc tggggacgct tgccacaaaa aacagtcttg
    15181 gtcccgctga cgttaatgcc ctggcgaagg atcagaacct tgccgacctg gagaatgcgg
    15241 aaaccgcccg taataatctg gatgtttaca gcaggagcga aggtgataac cgttacctgc
    15301 gcagggacca gaatggcgcg gatatccagg acaaaggaac ctttatcgat aacctcggtt
    15361 tacgggaaac ggtaaataag gctgctgatg ccctgccatc ggctggaacc gccgtggcag
    15421 caaacagact cgccacccca agaaatatta atggtgttcc ttttgacgga acgctggata
    15481 tcaacatcac gtcaggaatg acgcagtcaa ccgcagatgg ccggtatgta cagaatgttc
    15541 agcttggagc acagagctat cattcacccg gaggcaatga aatgtcatgg aattacagcg
    15601 caccttccgg ttgtatgctt tccggtatta acgtgcagga aaccggctcc cggtctgcgg
    15661 acaatatcgg tggggtctat tatcgcccgg ttcagattta tattaataat gcctggagaa
    15721 cggtaagttc agtgtaaacc acagaaaagg gtgctgaatg caccctctga attattccgg
    15781 tttttccggc cagggaatcg cctggtaatg cccctcactg gcaatatcat taaagtgcat
    15841 ttctttaagc tgtcggatat acgccatcca gcgcgtcagc tgtaatttat tttcatcgct
    15901 aatcatgtcc agttttaatt ctgttttcca gtcatccacg gtttcgttgg cttcctggag
    15961 cagcgatgag cgacgctttt cagcaaccgt cttccagttt accggcacct ttgatatcag
    16021 gccatcagaa aactgccagt taccgtcaat attcacacct tccggcagtt cgtccacttc
    16081 aacaacagta aaacctgccg gatataaagc agagacatct tcagcaacag agcagataat
    16141 acccgttaca ggcgaaatac agagtttgta tttctttgta aaaagcggga tagattcata
    16201 aaaatccctt ccgtcctcac tctggaaata ctgaacatcc tcaccatagg gtttgtcttt
    16261 cgggtaatat cgtttgacat taatcagttg cataaacatc accatgtaaa tcaatgataa
    16321 aaacaggaat aatactatcg ccatatacag gcaatgcttg tctgaaataa cgcatatttc
    16381 agatgctggc tgctgtgtgc tatgcccccg ttaattaaat acttgcagcg gatacccaat
    16441 gcccattaat acaatattgt atctgtctgt aataaacacg gtcatcacca ccacgcattt
    16501 cccagtctcc gtcagtctta aaaccggtaa tcacatttcc tgatgacagg gagtaattat
    16561 attccagtcc gccgcttaat gcctgcgctc ccagtcttac ccctgtaaca taacgctgat
    16621 tggccgttga ttccggcatt cctgacgtaa tgtttatatc ctgcgtgccg tcaaagacga
    16681 caccattgat tctgcgggca tttgccagcc tgcttgctgc aacagccgta ccgtttgatg
    16741 gcagggcatt cgcggccctg tttaccgtat cccgtaaacc gaggtttcag ggatgccgta
    16801 tatctgcttt aaaaggcttt tccacctctt tttaaggaag gtaaatacat gctgattggc
    16861 tatatacgcg tatcaacaaa tgaccagaac accggtttac aaagaaatgc gctggaatgt
    16921 gcaggatgtg agcagatttt tgaagacaaa atgagtggta aaacgacaca gcggccggga
    16981 ctgaagcggg cactgaagaa actaaaaccc ggtgacacgc tgatggtctg gaaactggac
    17041 aggctggggc gcagtatgcg ccaccttgtt gcgctgacag atgaattaca gcaacagggg
    17101 ataaattttc gtagtcttac tgacaacatt gatacgtcaa cccccatggg gcgcttcttt
    17161 tttcacataa tgggtgcgct ggcagaaatg gagcgtgaat taattgtcga acggacacgg
    17221 gctggtcttg cagctgcccg tgaaagggga cgaaccggcg gcagaccccg ccgtctcacg
    17281 caggaacaat acgagtaggt cgggcgttta ctggccgggg gtgtcagtcg aagacaggtg
    17341 gcacttattt ttgatatcgg tctttcaacc ctttatcgtt attatcctgc gagcgtttcg
    17401 gaaaaataag acgctctctt tgttgggcct ttactgaatt aacgcaaaat ggcgtttatc
    17461 ggtctgtgga tatgataatt cagtttattc aggaacaggc tgattattat gacgtccaaa
    17521 tgggttcaac tatcatcaat gcccggaaac tttactgtta aagtttccgg tggtacagcg
    17581 gcatttcttg aggctccctt tcctccggcc gaaacaaaag gaggaatgac atttgctgac
    17641 tgtctcatca gttttaatac acgggattgc ctgtgggtaa ggccagtatc cggtgatccg
    17701 agtgtggaaa ttaccggagc gggtattggt gcggtcattc cgttaagtgc tgatgttgcc
    17761 ggtactgctg aaccgtcaga ctgggataat gctgaaacac atacccgtcc gtcaggaaat
    17821 gaaactgctt ccagctcccc ttcctggtat tacgtggtgg ttcttgctgg tcagtcaaac
    17881 ggtatggctt acggtgaagg tctgccactg ccggagacat atgaccgccc cgaaccgcgt
    17941 attatgcagt tagcccgtcg cagtacagtc acgccgggag gtaaagcctg tcagtataac
    18001 gacatcattc tggccgacca ctgtctgcat gatgtgcagg atatgagtgg aaaaaatcac
    18061 ccgaaagcgg atgtggctaa agggcaatat ggcaccgtgg gtcaggggct tcacattgcc
    18121 aaaaaactgc tgccgtttat tccggcagat gcggggattc tgctggttcc ctgctgccgt
    18181 ggtggttctg cattcacggc gggtgctgac ggcacgtaca gtgacagcac gggtgcttca
    18241 gaggactcag cccgctgggg tgtggataag ccgctgtata aggacttaat cagccggaca
    18301 aaagcggcac tggcgaagaa cccgaaaaac cgcctgcttg ctgtggtgtg gatgcagggc
    18361 gagtttgata ttgatgcgaa gccgacggag cattctgcgc tgtttctggc gatggtggaa
    18421 aaattccgcg cagacctggc tgaacaggcg gaacagtgta ccggtggcag tgctgctggc
    18481 gttccgtgga tttgtggcga taccacgtat ttctggaaac agaaaaatga accagcatac
    18541 caggcaatct atggcggcta caaaaacaaa acagataaaa acattcattt cgttccatta
    18601 ataacagatg aaaatggcgt gaatgtaccc accaacaatc cggcagaaga cccggatatt
    18661 gaatccattg gttattacgg ctcaacgtgg cgtaacagtg ccgctacctg gacatctgcg
    18721 gaccgggcca gccatttcag tgcctgggcg cgccggggta ttatttctga ccgtctggcc
    18781 acggcagttc tgactcatgc agggcgaacc acagtaaaag ccgatgttcc atcttctgaa
    18841 actgaagcac cagtgccatc accttcagaa actgaagcag taaccacaac actgctgtct
    18901 taccgtgtca gtgagtcaga aggaaatctg
//
LOCUS       DAZGAP010000103        18856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGAP010000103 DAZGAP010000000
VERSION     DAZGAP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.103"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..857)
                     /locus_tag="ACHLAM_003096"
     CDS             complement(<1..857)
                     /locus_tag="ACHLAM_003096"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HFO7854586.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRY"
     gene            complement(1028..1666)
                     /gene="purN"
                     /locus_tag="ACHLAM_003097"
     CDS             complement(1028..1666)
                     /gene="purN"
                     /locus_tag="ACHLAM_003097"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO7854587.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(1666..2703)
                     /gene="purM"
                     /locus_tag="ACHLAM_003098"
     CDS             complement(1666..2703)
                     /gene="purM"
                     /locus_tag="ACHLAM_003098"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO7854588.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            3028..3654
                     /gene="upp"
                     /locus_tag="ACHLAM_003099"
     CDS             3028..3654
                     /gene="upp"
                     /locus_tag="ACHLAM_003099"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO7854589.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            3740..5029
                     /gene="uraA"
                     /locus_tag="ACHLAM_003100"
     CDS             3740..5029
                     /gene="uraA"
                     /locus_tag="ACHLAM_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO7854590.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            5124..5825
                     /gene="hda"
                     /locus_tag="ACHLAM_003101"
     CDS             5124..5825
                     /gene="hda"
                     /locus_tag="ACHLAM_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO7854591.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(5931..6290)
                     /gene="arsC"
                     /locus_tag="ACHLAM_003102"
     CDS             complement(5931..6290)
                     /gene="arsC"
                     /locus_tag="ACHLAM_003102"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO7854592.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(6311..7774)
                     /gene="bepA"
                     /locus_tag="ACHLAM_003103"
     CDS             complement(6311..7774)
                     /gene="bepA"
                     /locus_tag="ACHLAM_003103"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO7854593.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            7987..9048
                     /gene="yfgO"
                     /locus_tag="ACHLAM_003104"
     CDS             7987..9048
                     /gene="yfgO"
                     /locus_tag="ACHLAM_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7854594.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(9147..9617)
                     /gene="bcp"
                     /locus_tag="ACHLAM_003105"
     CDS             complement(9147..9617)
                     /gene="bcp"
                     /locus_tag="ACHLAM_003105"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO7854595.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(9617..10189)
                     /gene="gcvR"
                     /locus_tag="ACHLAM_003106"
     CDS             complement(9617..10189)
                     /gene="gcvR"
                     /locus_tag="ACHLAM_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO7854596.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            10335..11213
                     /gene="dapA"
                     /locus_tag="ACHLAM_003107"
     CDS             10335..11213
                     /gene="dapA"
                     /locus_tag="ACHLAM_003107"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO7854597.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASMKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            11230..12264
                     /gene="bamC"
                     /locus_tag="ACHLAM_003108"
     CDS             11230..12264
                     /gene="bamC"
                     /locus_tag="ACHLAM_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO7854598.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            12477..13190
                     /gene="purC"
                     /locus_tag="ACHLAM_003109"
     CDS             12477..13190
                     /gene="purC"
                     /locus_tag="ACHLAM_003109"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO7854599.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            13358..14221
                     /gene="ypfJ"
                     /locus_tag="ACHLAM_003110"
     CDS             13358..14221
                     /gene="ypfJ"
                     /locus_tag="ACHLAM_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO7854600.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            14236..16251
                     /gene="tmcA"
                     /locus_tag="ACHLAM_003111"
     CDS             14236..16251
                     /gene="tmcA"
                     /locus_tag="ACHLAM_003111"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO7854601.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDIVLSADDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            16325..17023
                     /gene="ypfH"
                     /locus_tag="ACHLAM_003112"
     CDS             16325..17023
                     /gene="ypfH"
                     /locus_tag="ACHLAM_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7854602.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     TATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(17104..17304)
                     /gene="ypfN"
                     /locus_tag="ACHLAM_003113"
     CDS             complement(17104..17304)
                     /gene="ypfN"
                     /locus_tag="ACHLAM_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO7854603.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVNHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17332..18459)
                     /gene="dapE"
                     /locus_tag="ACHLAM_003114"
     CDS             complement(17332..18459)
                     /gene="dapE"
                     /locus_tag="ACHLAM_003114"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO7854604.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(18463..18819)
                     /gene="yffB"
                     /locus_tag="ACHLAM_003115"
     CDS             complement(18463..18819)
                     /gene="yffB"
                     /locus_tag="ACHLAM_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO7854605.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         4355 a         4751 c         5060 g         4690 t
ORIGIN      
        1 tcgtagcggg aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg
       61 cgctgataaa caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatc
      121 aactccatca ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc
      181 ttcattgaat aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcgagg
      241 cagtaacgca gaatgttatc cagaagaatc atcggcttgc gtcgacgtgg cgcttctggc
      301 ggtaaattca caaagcgcgg cactttatct gatgggatct ccagcagcgc gtaacggatg
      361 gtatcgccac ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact
      421 aagtcagtgt caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa
      481 taatgacgca gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc
      541 tggttgcgcg ccatctccag caacagctcg ttgtagaggc cgtcgaattc ctgatcggct
      601 ttcagcaccc gggactgaat tttgcccagt aaatggcggg aatgagagtt ggaaccttgt
      661 tcttcgctaa taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca
      721 aggttattgg aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc
      781 gccgcttcct gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat
      841 agcttttcct gacccattac cacttttact ccgttttatt cacgggacac tgcctggata
      901 ttatggcgag caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct
      961 ggagcttgcg atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta
     1021 cggggcatta ctcgtcggca gcgtagccct gcggcggcag acgttgacca tccagccacg
     1081 cggcgttttc gtgcattttc agacgaccat cggcaaacca gctaatcacc agtggataaa
     1141 tagcgtgttc ctgggtttgc acgcgggcgg tgatgtcatc ttccgtatca ccagcaaata
     1201 ccgggacttt cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg acgaaatgca
     1261 ccgatgtacc gtgctcttca tcgccatttt ccagcgcctg acgatgggtg tgtaatccgg
     1321 gatatttcgg cagcagagaa gggtgaatgt tcagcaaacg cccggcatag tgggagacaa
     1381 acgccgggct gagaatgcgc ataaaaccag ccagcacgac cacatcgggt gcgtacatgt
     1441 cgatttcatg aatcaactcc cggtcatagg cttcacgact gtcaaacgcg ctggcgatga
     1501 gcgtatgcgt tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg tcggccttat
     1561 tgctgaaaac tgcccgtacg gtgcctttaa ttttgttggt tttgcaggcg tcaataattg
     1621 cctgcaaatt acttccgttg ccggaaataa gcaccacaat attcattatt cgataaccac
     1681 gcgttgttcg gaatcagagg ctttgatgat accgattttc cacgcgtttt caccgttggc
     1741 attgagcagg gcgagagctt tgtccacttc cggagcaggc agggcaataa tcataccgac
     1801 gccgcagttg aaggtgcgat acatttcatg gcgctcaaca ttacctgccg tttgcagcca
     1861 gttgaacact tccggccact gccaggaaga ttcatcaatc actgcctggg tattatctgg
     1921 caatacgcgc ggaatgtttt cccagaagcc gccgccggtc aggtgcgcaa tggcatgcac
     1981 atcgaccttt tcaatcaatt tcagcactga cttcacgtaa atgcgggtcg gtgccagcag
     2041 atgatcggct aatggcttac catcaagttc ggtggtttgc ggatcacaac cgctgacttc
     2101 aagaattttg cgcaccagcg aatagccatt cgagtgcggg ccgctggagc cgagtgcaat
     2161 cagcacatcg ccgtcgctga ctttagatcc gtcgatgatt tctgattttt ctaccacacc
     2221 aacgcagaaa ccagcgacat cgtaatcttc accgtgatac atccccggca tttccgccgt
     2281 ttcgccaccc accagtgaac agcccgattg cagacaacct tccgcaatgc cgctgatcac
     2341 cgctgaagcg gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg
     2401 ttccgcacct tgcaccacca ggtcattaac gcacatggcg accagatcaa taccaatggt
     2461 gtcgtgacgt tttaagtcca tcgccagacg cagcttggta cctacgccgt cagtgccgga
     2521 aaccagcaca ggttcacgat atttttgcgg caatgcacac agcgcaccga agccgcccag
     2581 accgcccatc acttccggac gacgcgtttt cttcactacg cctttgattc ttccaaccag
     2641 agcattaccc gcgtcaatat caacaccggc atctttgtag ctaagagagg ttttatcggt
     2701 cactgcttgg gtccccacgc gttacttgcg gtagaaaaat aaaattcggc gcaattctaa
     2761 cagggaaagc aaacgtttgc gagactgctt tacacaacct ttttgcacgt cttttcccca
     2821 ggcgcgcggc gaaagaatac ttgtactagg gcaaaggatc gttttcggat ggaataatct
     2881 tcttccataa ccatttgaat ataaaataac tttatctcaa accgttatca ttttgactaa
     2941 agtcaacgaa aataatattg ccgccttgaa gaaaggaggt ataatccgtc gatttttttt
     3001 gtggctgccc ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc
     3061 aaacacaagc tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc
     3121 gcttccgaag tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa
     3181 gtcactatcg aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt
     3241 accgttgtgc caattctgcg tgcgggtctt gggatgatgg acggtgtgct ggaaaacgtt
     3301 ccgagtgcgc gcatcagtgt cgtcggtatg taccgtaatg aagaaacgct ggagccggta
     3361 ccgtacttcc agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgaccca
     3421 atgctggcaa ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa agcgggctgc
     3481 agcagcatca aagttctggt gctggtcgct gcgccagaag gtatcgctgc gctggaaaaa
     3541 gcgcacccgg acgtcgaact gtatactgca tcgattgacc agggactgaa cgagcacgga
     3601 tacattattc cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata
     3661 aaaataatta aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca
     3721 taatacagag gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact
     3781 tttacagaca atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct
     3841 ggtgcccgtc ttatttcata ttaacccggc gactgtactg ttatttaacg gtataggaac
     3901 actgctgtat ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc
     3961 ctttatttca ccggtattgt tactgttgcc gttggggtat gaagttgcgc tgggcggctt
     4021 tattatgtgc ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg
     4081 ctggctggac gtgctgtttc cacctgcggc aatgggcgca atcgttgccg tcatcggtct
     4141 ggagctggcg ggcgtagctg ccggtatggc gggtttactc ccggctgaag ggcaaacgcc
     4201 agactccaaa accatcatca tctctattac caccctggcg gtcacggttt taggttccgt
     4261 gctgtttcgt ggtttcctgg caattatccc gattttaatt ggcgtgctgg tggggtacgc
     4321 gctctctttc gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc
     4381 gctgccgacc ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctaccagc
     4441 ggcgttagtg gttattgccg aacacgtagg gcacctggtc gtaacggcta atatcgtcaa
     4501 aaaagatctg ctgcgcgatc caggtctgca ccgttcgatg tttgctaacg gcttgtcgac
     4561 cgtgatctcc ggcttctttg gctccacgcc aaacaccact tacggagaaa acattggcgt
     4621 gatggcgatc acccgtgttt acagtacctg ggttatcggc ggtgcggcga ttttcgctat
     4681 cctgctttcc tgcgtcggta aactggctgc agctatccag atgattccat tgccggtgat
     4741 gggcggcgtt tcgctgctgc tttacggtgt catcggtgct tccggtattc gtgtgttgat
     4801 cgaatcgaaa gtggattaca acaaagcaca gaacctgatc ctgacttccg ttattttgat
     4861 catcggcgtc agtggcgcga aggtaaacat tggcgcggcg gagctgaaag gtatggcgct
     4921 ggcgaccatc gtcggtatcg gcttaagttt gatcttcaaa ctgattagta tgctgcgccc
     4981 tgaagaagtg gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg
     5041 ggcagtgttg ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt
     5101 gtgaaatcct ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc
     5161 ttcctgacga cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggccg
     5221 cgctgcaaaa cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag
     5281 gcgcggggcg cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg
     5341 cggtgggcta tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta
     5401 tggagcattt gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt
     5461 gggagatggc gattttcgat ctctacaatc gaattctgga atcgggcaaa acacggttgt
     5521 taatcaccgg cgatcgtcca ccgcggcagt tgaatcttgg attaccggat ctcgcgtcgc
     5581 gtctcgactg ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg
     5641 cgctacagtt acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct
     5701 tgctgaagcg gctcgacaga gagatgcgca cgttatttat gacgttggat cagttggatc
     5761 gtgcgtcgat taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaaac
     5821 tgtagacaag acgtttattg tcggatatgc acccatagta ttcgtcggcc ggataagacg
     5881 cggcaagcgt cgcatccggc aatgtgcttc aactagcgat ggagcacttc ttaacccacg
     5941 atttccagta cctgctccgg tggacgacca atccgcgctt tgccattcgc gaccacaatc
     6001 gggcgctcca ttagcttcgg attatccacc attgcctgaa tcagcgcctc ttcgctaagc
     6061 gaactgtcag ccaggttcag ttctttataa agatcctctt tctggcgcat caattcacga
     6121 gcgctattca tccccagcat tttcagcaaa tcacgcagcg ttgccgcatc ggcgggtgtc
     6181 tcaaggtaga gcaccacttc tggctccacg ccgttttctt tcagcaggtt cagcgtttcc
     6241 cggctctttg aacagcgtgg gttatggtag atttttacct gtttggtcat tgttcttcct
     6301 ttaatgcgaa ttacatcttg gtataaggct taaagcgttc ctgcagctgg cgcaactggt
     6361 cgatgcgcgc atcgtaacgc gcttgttgca ggctgcctaa tttcacctgc gaactggcgc
     6421 tactcaacag cgaaatggcc tgatcgagtc gtccggcgag cgcataacct tctgctcgcg
     6481 cagccagctc ctgatcgcgg ttatttagcg cggcttccgc ctgtgccagc aaatcccagc
     6541 cgttgctgtc atctttatta ttaaaggtgt agcgattcag aatattggcc gcttcttgtg
     6601 gttgaccgcc ttgcagataa gcgttcgcca ggttgagctg caacaccgga ttggtgcgta
     6661 aatcgcgggc atttttcaga cgattgatcg cctcattggc tttgttttgc ccaagatcga
     6721 tatcagtagc cagatcgaga taccatgcgt tgccaggttc tgccgccagt aacggttgca
     6781 gcgttttacg cgcctcgtcg tatttattgg cttccatcgc ctgtaaagca cgaccatatt
     6841 gcgccgctcg ttgctgacga acgtttcctt tcgcccattc atccagcaaa tcactggtga
     6901 gctggttacg tccggaatta tacatcccca gtgtgcgcgc tttcgccaga tagaaatctt
     6961 ccgacgactg caccaccatc gggcgcatct gattagcacg gttgcgggca tctgccagac
     7021 gactttccgg caacgggtga gtcaataaaa tttccggcgg acgcgaggag taacgcgcct
     7081 gatcgagtaa tttttcgagg aaggtcggca tcgcctgcgg atcgaatcct gagcgttgca
     7141 gcacctgaat accaatgcgg tccgcttcct gttcattttg ctgggtgaag ctgatcatcc
     7201 cctggcgcgt tcccgccagt gtaccggtca gcgcagccat ccccgcctgc ggactggcca
     7261 tcgccagtaa aatagaacct aacgcgccga cccaggtcag cggcgcgctg cgctgctgat
     7321 cttccatcgc tcgcgccagg tgacgttggg tgacgtggga gatttcgtgc gccataactg
     7381 aagccagttg actttcgtta tcggaataac ggaacagggc agagtgcagc accacgttgc
     7441 cgccaaagaa agcaaaggcg ttaatttcat cgttgttgat cagaaagaaa tgaaacggtg
     7501 tcttaaccga attggcatgc gaaaccagac gcattcctag cgaattaatg tattgcgtta
     7561 acagcggatc attaattaac ggcgcgctgc cgcgtagctg gcggacataa tagtcgccca
     7621 tctgcatttc ctgaccaatg gaaagtgtgc ttcctgcgga ggttcccata tccggcaagg
     7681 tgtctgcgct gtcggcaaac gccggggcta cctgaccaat ggtcatagca gcaatgaggg
     7741 ttgcaaccag gttttttttc aactgcctga acataacctc tatcctgtat ttctggagat
     7801 agtcatttga ccgatactgt gaaatatcgt tcccaccgcg ccgcctttga gtgtaacggc
     7861 gaagattagc ccactcaaat gacttttcag tttcctgaat ccgtcataaa aagcgaagtc
     7921 ttttttgtgg catttcgata caattgcgga tcgcaatccc gctcttgagg ttcagggaag
     7981 gtctgtatgc tcgaaatgtt gatgcaatgg tatcgccgcc gttttagcga cccggaagcg
     8041 attgccttgc tggttatttt agttgccgga tttggcatta tctttttctt tagtggcctg
     8101 cttgctccgc tgttggtggc tattgtgctg gcctatttgc tggaatggcc aaccgtgcgc
     8161 ctgcaatcta ttggctgctc ccgccgctgg gcgacgtcga ttgtattggt ggttttcgtc
     8221 ggtatattgc tactgatggc gttcgtggta ctgcctatcg cctggcagca gggcatctac
     8281 ttaatccgcg atatgccggg gatgctcaat aagctttctg actttgccgc cacgttgccg
     8341 cgccgctatc cggcgttaat ggatgcgggc attattgatg caatggccga aaatatgcgc
     8401 agtcggatgc tgaccatggg cgattcggtg gtgaaaattt ccctcgcctc gctggtcggt
     8461 ttgctgacca ttgccgtcta tctggtgctg gtaccattga tggtcttctt cctgctgaaa
     8521 gacaaagagc agatgctgaa cgccgttcgc cgggtgctgc cgcgtaaccg tggactggca
     8581 ggacaggtgt ggaaggagat gaatcaacaa atcaccaact atatccgcgg caaagtgctg
     8641 gagatgatcg tggtggggat tgccacctgg ctgggattct tactcttcgg gctgaactat
     8701 tcgctgctgc tggcggtgct ggtcggcttc tcggtactta ttccgtacat tggtgcattt
     8761 gtggtgacca ttccggtggt tggcgtggcg ctattccagt ttggtgctgg cacggaattc
     8821 tggagctgct tcgcggtgta tctgatcatt caggcgctgg acggcaacct gttagtacct
     8881 gtgttgttct ccgaagcggt taacctgcat ccgctggtca ttattttatc agtagtgatc
     8941 ttcggtggtt tgtggggatt ctggggcgta ttcttcgcca ttccattggc gacgctgatc
     9001 aaagccgtga ttcacgcctg gcccgatggg caaatcgcgc aagactgacc ttcagcgaaa
     9061 aaatttgaaa cgagtgttgc agggcagtga gaagatgtga gatgtgctgg ccgcaagcgc
     9121 agccagcacg gaatggagca aagtaatcag gcgtgttctt tcagccagtt cagcacaacg
     9181 tcgtggtgat tgctggtttt gaaatcgtca aagacatgtt cgattttgcc atcagcgtca
     9241 atcaggaagc tgatgcgatg aataccatcg taggttttgc ccatgaagga cttttcaccc
     9301 cagacgccga attgttcgca cacctggtgg tcctcatcag acaggagcgt aaagttaagc
     9361 agctcttttt ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat acccagcaca
     9421 tcaacgcccg cttttttcaa ctcatccatg ttatcgcgta agccgcaggc ctgtacggta
     9481 cagccggggg tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc ctggaagtcg
     9541 gtcaaattaa cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc gatatcaccg
     9601 gctttcagtg gattcattac ttaactccat cctgttcatc atgttgggaa taattgacga
     9661 cgttaatact gccttgtgca ttgagttctg tacatagggc tttgaatgct tgctcaatat
     9721 ttgctgcgtc cgcagatgcg gggctgtggg cggttatctg aatatgcaac tgcgcagccc
     9781 gttcattttc agcaggttgc gtgcgcgaca ccagctccgc aatgttcata tgatgcgcgt
     9841 cgaaaagtgc tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg acctgaaccc
     9901 agacagatgc tggcattggc ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa
     9961 gatccagctc ggcacctttc aacggtaacg ttgattcaat cagagtaatg gcattccatg
    10021 aaccggaaag cagcatgata aacgtgaact cttctcccag catcgccagg cgactgtctt
    10081 caatattaca gccgcaacta ctgacatgac gggtgatggt gttcacaatt ccagggcgat
    10141 cggcacccaa cgcagtgatc accagataat gttgcgatga cagtgtcaaa ctggttattc
    10201 cttcaagggg tgagttgttc ttaaggaaag cataaaaaaa acatgcatac aacaatcaga
    10261 acggttctgt ctgcttgctt ttaatgccat accaaacgta ccattgagac acttgtttgc
    10321 acagaggatg gcccatgttc acgggaagta ttgtcgcgat tgttactccg atggatgaaa
    10381 aaggtaatgt ctgtcgggct agcatgaaaa aactgattga ttatcatgtc gccagcggta
    10441 cttcggcgat cgtttctgtt ggcaccactg gcgagtccgc taccttaaat catgacgaac
    10501 atgctgatgt ggtgatgatg acgctggagc tggctgacgg gcgcattccg gtgattgccg
    10561 ggaccggtgc taacgctact gcggaagcca ttagcctgac gcagcgcttc aatgacagcg
    10621 gtatcgtcgg ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt
    10681 atcagcattt caaagccatc gctgaacata ctgacctgcc gcaaattctg tataatgtgc
    10741 cgtcccgtac tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa
    10801 atattatcgg aatcaaagag gcaacaggga acttaacgcg tgtaaaccag atcaaagagc
    10861 tggtttcaga tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc
    10921 aattaggcgg tcatggggtt atttccgtta cggctaacgt cgcagcgcgt gatatggccc
    10981 agatgtgcaa actggcagca gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc
    11041 tgatgccatt acacaacaaa ctatttgtcg aacccaatcc aatcccggtg aaatgggcat
    11101 gtaaggaact gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg
    11161 acagtggtcg tgagacggtc agagcggcgc ttaagcatgc cggtttgctg taaagtttag
    11221 ggagatttga tggcttactc tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg
    11281 cttgttttat tactcgctgc ctgtagttct gactcacgct ataagcgtca ggtcagtggt
    11341 gatgaagcct acctggaagc ggcaccgctt gcggagcttc atgccccggc tggaatgatt
    11401 ttgccggtga cctccggcga ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt
    11461 aaggcgctgg acattcgtcc accagcccag ccgttggcac tggtttctgg cgcgcgtacc
    11521 cagttcacgg gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg
    11581 ccgcaggtgg ttagcgtgct gcaggcgaaa aactacacca tcacccaacg tgatgatgct
    11641 ggtcagacac tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat
    11701 cgtggtcgtt atcaaatctc tgttaagccg cagggttatc agcaggcggt tacggttaaa
    11761 ctgctgaacc tggaacaggc gggcaaaccg gttgcagacg cggcttccat gcagcgttac
    11821 agcacggaga tgatgaacgt tatttccgcc ggtctggata aatctgccac tgacgccgcg
    11881 aacgctgcgc aaaatcgtgc ctccaccact atggacgtac aaagtgcagc tgatgacacc
    11941 ggtttaccaa tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg tctgccagct
    12001 gcgctggaaa aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg caacatggcc
    12061 gtaacttaca agccgctgtc tgacagcgaa tggcacgaac tgggcgcgag cgatccaggt
    12121 ctggcatccg gtgactataa actgcaggtt ggcgatttag ataaccgcag cagcctacag
    12181 ttcatcgatc cgaaaggtca tactctgact cagagtcaga acgacgcgct ggtagctgtc
    12241 ttccaggctg cgtttagcaa gtaaaaatac agggctggag tcatccggcc cttttttctg
    12301 atatgatacg caaacgtgtg cgtcggcaga aaaacgcgat tttagcggta attcgcacga
    12361 aatttgtttg tcggacgtag gtcggataag gcgttcacgc cgcatccgac aaaacatccg
    12421 gcacaccaga cagcaataga ttttaaaacg ttaattcaca cccaggagtg ataaagatgc
    12481 aaaagcaagc tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg
    12541 acctgttggt gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg cgcattgagc
    12601 agtttgatcg caaaggtatg gtgaacaaca agttcaacta cttcattatg agcaaactgg
    12661 ctgaagcggg tatcccgact caaatggagc gtctgctctc cgataccgaa tgtctggtga
    12721 aaaagctgga tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct ggctctctgg
    12781 tgaaacgtct tggaatcgaa gaaggtattg agctgaaccc gccactgttc gatctgttcc
    12841 tgaaaaacga cgccatgcac gatccgatgg tcaacgaatc ttactgcgaa acctttggct
    12901 gggtaagcaa agagaacctg gcacgcatga aagagctgac ctacaaagcg aacgacgtgc
    12961 tgaaaaaact gttcgatgat gctggtctca ttctggtcga cttcaagctg gagtttggtc
    13021 tgtacaaagg tgaagtggta ctgggcgatg agttctcccc ggacggcagc cgcttgtggg
    13081 acaaagaaac gctggagaaa atggacaaag accgtttccg ccagagcctc ggtggcctga
    13141 tcgaggccta tgaagccgtc gcccgccgcc tgggtgtaca gctggactga tttttctgtt
    13201 catcatcttg ccgtgcagct ggcacggcaa gacaaccgct ctcataagat gagtattgaa
    13261 cgctcattcc tccgccattt catcccgctt tcctgtggta atcttcctct gaaccaccta
    13321 cgatcatttc tataatgaat atattgttga ggtatctatg cgttggcaag ggcgacgtga
    13381 aagtgacaat gttgaagaca ggcgcaacag ctctggtggt ccgtcaatgg gcggtccagg
    13441 ttttcgcctg ccaagcggta aaggcgggct gattttactg atagtcgtgc tggttgcagg
    13501 ctactatggc gttgatttaa ccgggttgat gaccggacag ccggtttccc aacagcaatc
    13561 aacacggtca attagtccta atgaagacga agccgcaaaa ttcacttcgg tgattctggc
    13621 aaccacggaa gacacctggg gacagcaatt cgagaagatg ggtaaaacct atcagcaacc
    13681 gaagctggtc atgtaccgtg gaatgacgcg caccggctgc ggggcgggcc agtccataat
    13741 gggaccgttc tattgcccgg cggatggcac ggtttatatc gatctctcct tctatgatga
    13801 catgaaagac aaacttggcg cggatggcga ttttgcccag gggtacgtta tcgcccatga
    13861 agtcggtcat catgtgcaga aactgttagg catcgagccg aaagttcgtc aactgcaaca
    13921 aaacgcgacg caggcggaag tgaaccgctt atctgtgcgt atggaactcc aggccgactg
    13981 ttttgccggt gtctgggggc atagtatgca gcagcaaggt gttctggaaa ccggcgatct
    14041 ggaagaggcg ctgaacgcgg cgcaggccat cggcgatgac cgtttacaac agcaaagtca
    14101 ggggcgagta gtaccagaca gtttcactca tggcacttct cagcaacgct acagctggtt
    14161 taaacgtggt ttcgacagcg gcgatccggc acaatgcaat acttttggta aaagcattta
    14221 acttccgggg cagggatggc tgaactgact gcgcttcaca cattaacagc gcaaatgaaa
    14281 cgtgaaggga tccgccgctt gctggtgttg agcggggaag agggttggtg ttttgatcat
    14341 gcgcttaagt tgcgtgatgc cttacctggc gactggctgt ggatttcgcc gcagccagat
    14401 gctgaaaacc actgttctcc ctcggcgcta caaactttac ttgggcgcga gttccggcat
    14461 gcggtattcg acgcccgcca cggctttgat gccgctgcct ttgcggcact tagcggaacg
    14521 ttgaaagcgg gaagctggct ggttttgtta ctccctgtat gggaagagtg ggaaaaccaa
    14581 cctgatgccg actcgctgcg atggagtgat tgccctgacc ctattgcgac gccgcatttt
    14641 gtccagcatc tcaaacgcgt acttacggcg aataacgacg ctatcctctg gcggcagaac
    14701 cagccgttca cgttggcgca ttttactccc cgtactgact ggcaccccgc gaccggcgca
    14761 ccacagccag aacaacagca actcttacag cagctactga ccatgccatt gggcgtggcg
    14821 gtggtaacgg ctgcgcgtgg gcgcggtaaa tcggcgctgg cagggcaact catttctcgt
    14881 attgcgggta gtgcgattgt caccgcgccc gcaaaagcgg caacgtatgt actggcacaa
    14941 tttgcgggcg agaagtttcg ctttattgcg ccggatgcct tgttagccag cgatgagcaa
    15001 gccgactggc tggtggtcga tgaagccgca gccatacctg cgccgttgtt gcatcaactg
    15061 gtatcgcgtt ttcctcgaac gttgttaacc actacggtgc agggctacga aggcacggga
    15121 cgtggttttt tgctgaaatt ttgcgctcgc tttccgcatt tacaccgttt tgaactgcaa
    15181 cagccgatcc gctgggcaca gggatgcccg ctggaaaaaa tggttagtga ggcactggtt
    15241 tttgacgatg aaaacttcac ccacacacca caaggcaata tcgtcatttc cgcatttgaa
    15301 cagacgttat ggcgaagcga gccacaaacg ccgttaaagg tttatcaatt attgtctggt
    15361 gcgcactacc ggacttcgcc gctggattta cgccgcatga tggatgcacc agggcaacat
    15421 tttttacagg cggctggcga aaacgcgatt gccggggcgc tgtggctggt ggatgagggc
    15481 ggattatctc aagaactcag tcaggcggta tgggcaggtt ttcgtcgccc gcggggtaat
    15541 ctggtggccc agtcgctggc ggcgcacggc agcaatccac tggcggcgac attgcgcgga
    15601 aggcgggtta gccggatagc agttcatccg gctcgtcagc gggaaggcac aggtcagcaa
    15661 ctgattgctg gtgctttgca atatacgcat gacctcgact atctttcggt gagttttggt
    15721 tacaccgggg aattatggcg tttctggcaa cgctgcggtt ttgtgctggt gcgaatgggt
    15781 aatcatcgtg aagccagcag cggttgctat acggcgatgg cgctgttacc gatgagtgat
    15841 gcgggtaaac agctggctga acgtgagcat taccgtttac gtcgcgatgc gcaagctctc
    15901 gcaaagtgga atggcgaaac gctccctgtt gatccactaa acgatatcgt gctttctgcc
    15961 gacgactggc ttgaactggc cggttttgct ttcgctcatc gtccgctatt aacgtcgtta
    16021 ggttgcttaa tgcgtctgtt acaaaccagc gaaatggcat taccggcgct gcgtgggcgt
    16081 ttacagaaaa acgccagcga cgcgcagtta tgtaccacac ttaaactttc aggccgtaag
    16141 ctgttactgg tccgtcagcg ggaagaggcc gcgcaggcgc tatacgcact tgatgatgtt
    16201 cgcactgagc gtttgcgcga tcgcataacg caatggcaat tttttcactg actccttcaa
    16261 ttttctccca tggtcattgt gagtcagcgg cgtaaaatta atgacatcag ttaaggagtt
    16321 cgccatgaaa catgaccatt ttgttgttca aagcccggat aaacctgcgc aacagttgtt
    16381 actgcttttc cacggtgtcg gggataaccc ggtggcaatg ggggaaatag gtagctggtt
    16441 tgcgccgcta tttcctgatg cgctggtggt gagtgtcgga ggcgcggagc cgagcggtaa
    16501 tccggcgggg cgtcagtggt tttcggtgca gggtattacg gaagataatc gccaggcgcg
    16561 tgtggatgcc atcatgccga cgtttattga gacggtgcgc tactggcaga aacagagcgg
    16621 agtaggggct accgccacgg cgttaatcgg tttctctcaa ggcgcgatta tggcgctgga
    16681 gagcattaaa gccgaaccgg gccttgcctc acgcgtcatt gcctttaacg gacgctatgc
    16741 gagcctgccg gaaactgcgt ctacagccac gacaattcac ctgattcatg gcggtgaaga
    16801 tccggttatc gatctggcgc atgcggttgc ggcgcaggaa gcgttaatca gtgccggtgg
    16861 tgacgtgacg ctggatatcg tggaggattt aggccatgcg atcgacaatc gcagtatgca
    16921 atttgctctg gatcacttgc gttataccat tccgaaacat tatttcgacg aagcgctaag
    16981 cggcggtaag cctggcgatg atgatgtgat tgaaatgatg taataatagc cgggtaggtc
    17041 tgataagact gtatcaggca tcatggccgg atgcggcgtt aacgccttat ccggccactt
    17101 agcttacttc ttcggttgat cctttttcgg ccagtcgtcg tcatcgtccc acttatcgtt
    17161 gaaatcacga tgcgggggaa gctccggctt gttggcgaga aatttcttat ggttgacgcg
    17221 cttgagatct ttaatcacgt tcagcaggac gcctaccaaa aagacaatca ccagaatcca
    17281 ccaatattta gccagccagt ccatgcttat ttcctcttgc agaaccactc atcaggcgac
    17341 gagctgttcc atgatacgtt gatacatacg ggcaagtagc tgcaggtcgg cagcgttcac
    17401 acattcatta attttatgaa tagtggcatt gaccggcccg agttccacca cctgcgcccc
    17461 catgcgggca ataaagcgcc cgtcggacgt tccgcctgtg gttagtagct gcggtttaat
    17521 ttcattatag tgctcaaccg cgttaacgac cgcatccacc agtttaccgc gcgcggtcaa
    17581 aaatggctgc ccggaaagcc accaatccac cgtatagcgc agttgatgtt tttcaagcag
    17641 ggcaagtacc tgcgctttga tcatctcatc agtcagttcg gtgctgaagc ggaagttaaa
    17701 ctgcacaaac agttcacccg gaataacgtt gttactgccc gttcccgcct gaatattggc
    17761 aatctgcata ctggtcgccg ggaagaattc attgccctga tcccactcaa tagccactaa
    17821 ttcattaagg aaaggtgctg cgcgatgtac cggattgtca gccagatgtg ggtaggcaac
    17881 atgcccctgc acgccatgaa tggtgaggtt gcaggttaac gatccgcgac gaccattttt
    17941 caccacatca ccaaccactt cgatactcga cggttcgcca accagacagt aatcgagacg
    18001 ctcattacgc gccattaacg cttcgacgac ttttaccgta ccgttgtggg cactagcttc
    18061 ttcatcagag gtgatcagaa atgccagtcg ccccgtatgg ttgggatgtt gtgcgacaaa
    18121 acgttctgcc gctaccacca tcgccgccag cgagcctttc atatctgccg caccgcgccc
    18181 gaataacatg ccgtcacgaa tggtgggttc aaacggcgga ttgatccaac ggtcggcgtc
    18241 gccaggcggc accacgtcgg tatgcccggc aaaggctaac gtttcaccct gcccacgcca
    18301 tgcccaaaaa ttctgcgtat cggcaaagtc catgcgttca acggtaaaac cgatcgcctg
    18361 caaacgttca atcaacaaag cctggcatcc tgcgtcatca ggactcaggg aagggcggcg
    18421 aataagctgt tgtgtcagct caataaccgg gcacgacata gactacacct catggaaaaa
    18481 ttgctgataa ctggattcac tgaaacccag cagcataggc ttaccaggcg cgcagagcaa
    18541 tggacgtttg ataattgcag gcatttcagt cattaatgtc gccgcagagg ccgcatcggt
    18601 gattttattg cgggtggttt cgtccagttt acgccaggtt gtaccacggg tgttgagtaa
    18661 cgcttcccag cctaattcgt tgataaaacc gttcaataat tcgctgtcca atccatcgac
    18721 gcggtaatca tgaaagcgat agtcgatgtt attggcttcc agccaacggc gagccttttt
    18781 aatggtgtca caatttttga tgccgtaaag tgtaaccata atgaatcctt tttcgattta
    18841 tgagaataaa tactca
//
LOCUS       DAZGAP010000104        18734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGAP010000104 DAZGAP010000000
VERSION     DAZGAP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.104"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            158..463
                     /locus_tag="ACHLAM_003116"
     CDS             158..463
                     /locus_tag="ACHLAM_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854606.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            570..1214
                     /gene="gfcB"
                     /locus_tag="ACHLAM_003117"
     CDS             570..1214
                     /gene="gfcB"
                     /locus_tag="ACHLAM_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO7854607.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1211..1957
                     /locus_tag="ACHLAM_003118"
     CDS             1211..1957
                     /locus_tag="ACHLAM_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7854608.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1957..4053
                     /locus_tag="ACHLAM_003119"
     CDS             1957..4053
                     /locus_tag="ACHLAM_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7854609.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4099..5238
                     /locus_tag="ACHLAM_003120"
     CDS             4099..5238
                     /locus_tag="ACHLAM_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO7854610.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5226..5672
                     /gene="etp"
                     /locus_tag="ACHLAM_003121"
                     /gene_synonym="yccY"
     CDS             5226..5672
                     /gene="etp"
                     /locus_tag="ACHLAM_003121"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO7854611.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5692..7872
                     /gene="etk"
                     /locus_tag="ACHLAM_003122"
     CDS             5692..7872
                     /gene="etk"
                     /locus_tag="ACHLAM_003122"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO7854612.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEGFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(7992..9290)
                     /gene="appA"
                     /locus_tag="ACHLAM_003123"
     CDS             complement(7992..9290)
                     /gene="appA"
                     /locus_tag="ACHLAM_003123"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO7854613.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQCLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFSQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9370..9462)
                     /gene="cbdX"
                     /locus_tag="ACHLAM_003124"
     CDS             complement(9370..9462)
                     /gene="cbdX"
                     /locus_tag="ACHLAM_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO7854614.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9475..10611)
                     /gene="appB"
                     /locus_tag="ACHLAM_003125"
     CDS             complement(9475..10611)
                     /gene="appB"
                     /locus_tag="ACHLAM_003125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7854615.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10623..12167)
                     /gene="appC"
                     /locus_tag="ACHLAM_003126"
     CDS             complement(10623..12167)
                     /gene="appC"
                     /locus_tag="ACHLAM_003126"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO7854616.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12301..13158)
                     /locus_tag="ACHLAM_003127"
     CDS             complement(12301..13158)
                     /locus_tag="ACHLAM_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO7854617.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQISERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            complement(13155..13553)
                     /gene="hyaE"
                     /locus_tag="ACHLAM_003128"
     CDS             complement(13155..13553)
                     /gene="hyaE"
                     /locus_tag="ACHLAM_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO7854618.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13550..14137)
                     /gene="hyaD"
                     /locus_tag="ACHLAM_003129"
     CDS             complement(13550..14137)
                     /gene="hyaD"
                     /locus_tag="ACHLAM_003129"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO7854619.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPAKESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            complement(14134..14841)
                     /gene="hyaC"
                     /locus_tag="ACHLAM_003130"
     CDS             complement(14134..14841)
                     /gene="hyaC"
                     /locus_tag="ACHLAM_003130"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO7854620.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14860..16653)
                     /gene="hyaB"
                     /locus_tag="ACHLAM_003131"
     CDS             complement(14860..16653)
                     /gene="hyaB"
                     /locus_tag="ACHLAM_003131"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO7854621.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLSDDGSELISVQVR"
     gene            complement(16650..17768)
                     /gene="hyaA"
                     /locus_tag="ACHLAM_003132"
     CDS             complement(16650..17768)
                     /gene="hyaA"
                     /locus_tag="ACHLAM_003132"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO7854622.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     KMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18195..18282
                     /locus_tag="ACHLAM_003133"
     tRNA            18195..18282
                     /locus_tag="ACHLAM_003133"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18229..18231,aa:Ser,seq:tga)
     gene            18422..>18734
                     /locus_tag="ACHLAM_003134"
     CDS             18422..>18734
                     /locus_tag="ACHLAM_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7854623.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KY"
BASE COUNT         4585 a         4983 c         4855 g         4311 t
ORIGIN      
        1 taacaatctt atatttccct ctgcgcgcct ttcccacccg cagtatatag tgctggcggt
       61 atgaggaaag ggagatttca caaggatgtt atactcaaag tagataatcc tgatctcaag
      121 cgtacgtatt gtccatgcga tgaataaagg aaaagttatg aaacacaagc tttctgcaat
      181 ccttatggcc ttcatgttga cgacgccagc tgcatttgct gcccctgaag cagcaaacgg
      241 gactgaggca acgacgggta ctactggcac cacaacgacc actacaggtg caaccacgac
      301 tgctgctacc actggtggcg tggctgctgg cgctgtcggc acggcgacgg ttgtcggtgt
      361 ggcaacggct gttggtgtgg cgacgttggc agtagttgca gcaaatgaca gtggcgatgg
      421 cggttctcat aacacctcaa ccaccacgtc cactacgcgc taatactgct ttaacgcgtc
      481 aaaattaaag ttaaatgata cccggttgcg atattcgtac cgggctttag agatttgcat
      541 ttttttaatt aacctttcaa ggattaaagg tgcgccctct tattttatcg attttcgcac
      601 tatttcttgc gggatgtacg cacagccagc aaagtatggt cgatacattt cgcgccagcc
      661 ttttcgataa tcaggatatc accgtagcgg atcagcagat ccaggcgttg ccttattcca
      721 cgatgtattt acgccttaat gaagggcaac gaatctttgt ggtactggga tatatagaac
      781 aagaacaaag caaatggtta tcccaggata acgccatgct ggttacccac aatggacgtc
      841 ttttaaaaac cgtcaaactt aataataatc tgctggaagt gacaaattcc gggcaggacc
      901 ctctgcgtaa cgcgctggca ataaaagacg gcagccgctg gacgcgcgat attctctgga
      961 gtgaagacaa ccattttcgc tctgcgaccc tgagttccac tttttccttt gctggattag
     1021 agacgctgaa tattgcgggt cgcgatgtgc tgtgtaatgt ctggcaggaa gaggtgactt
     1081 ccacgctgcc agaaaaacag tggcaaaaca cattctgggt cgattcggct actggccagg
     1141 ttcgtcaaag tcggcaaatg ttaggcgcag gggttattcc cgtagaaatg acgtttctta
     1201 aacccgcacc atgaataaat tacagtcgta tttcattgcc agcgtacttt acgtaatgac
     1261 accccatgcc tttgcgcaag gaacggtgac tatttatctg cctggcgaac aacagacact
     1321 ttccgttggc cccgtggaaa atgttgtcca gttggtgaca caaccgcaac tgcgcgatcg
     1381 cctctggtgg cctggtgcct tgctgaccga ctctgcggcg aaagctaaag cgcttaaaga
     1441 ctaccaacat gtcatggcac agcttgcttc ctgggaagca gaagctgacg acgatgtggt
     1501 ggcaacgatt aaatccgtgc gccagcagtt actcaatctc aatatcactg gtcgcttacc
     1561 ggtcaaactg gaccctgatt ttgtacgagt ggatgagaac agcaatcccc cgctggtcgg
     1621 ggactatacg ctctataccg tacagcgtcc ggtgactatt actctgctag gtgcggtttc
     1681 tggggctggg caattgccct ggctggcagg ccgtagcgtt accgattatc tgcaagatca
     1741 cacccgtctt gcaggcgcgg acaagaataa cgttatggtg attacgcctg agggtgaggc
     1801 tgtcgtcgcc cctgttgccc tgtggaataa aagacatgtg gaaccaccgc ctggcagcca
     1861 gctttggtta ggcttttctg cgcatgtact gcccgaaaaa tatgctgatt taaatgacca
     1921 gattgtgtct gttttaacgc aacgtgttcc tgactgatga agaagaactc ttatctttta
     1981 agctgcctgg ccattgccgt ctccagtgcc tgtcatgctg aagtattaac ctatccggat
     2041 ccgctgggtt cgtcgcaatc agactttggc ggcacaggat tgttgcagat gccaaatgcg
     2101 cgcatcgcac cggaaggtga attcagcgta aactaccggg ataacgatca ataccgtttc
     2161 tactccacct ccgtggcgct gttcccatgg ctggaaggca ccattcgtta tacggatgtg
     2221 cgcacacgca aatatagcca gtgggaagat ttcagcggcg atcagtcata caaagacaaa
     2281 tctttcgatt ttaaacttcg cctgtgggaa gaaggttact ggctaccgca agtggcgttt
     2341 ggtaaacgtg atattgctgg tacgggtctg tttgacggtg aatatttggt ggccagcaag
     2401 caagcgggcc cgttcgattt caccctcggg atggcctggg gctacgccgg taatgcgggc
     2461 aatattacca acccgttttg tcgggtaagc gataaatatt gccatcgcgc agagtctcac
     2521 gatgcgggag atatcagctt tagcgatatc tttcgtggtc cagcctctat ttttggcggt
     2581 attgagtatc aaacgccgtg gaatcctctg cgtctgaaac tcgaatacga tggcaacaat
     2641 taccagaatg attttgcggg caaactgcct caggcaagcc atttcaacgt cggcgcagtc
     2701 tatcgcgctg ccagctgggc agatctcaac ctgagttatg aacgcggtaa cacgttgatg
     2761 tttggcttca cgttacggac caatttcaac gatctgcgcc ctgccctgcg cgatacgcca
     2821 aaaccggcat atcaacctgc gccggaatct gaaggattgc agtacaccac ggtagcaaac
     2881 caacttaccg ccctgaagta taacgcgggc tttgacgcgc cagaaattca gctacgcgat
     2941 aagacgctgt atatgtctgg tcagcaatac aaataccgtg actctcgcga agcggtcgat
     3001 cgtgccaacc ggattctggt gaataacctg ccgcaaggcg ttgagaagat tagcgtgacg
     3061 caaaagcgcg agcatatggc gatggtgact accgaaaccg acgtagccag cctgcgcaaa
     3121 caactggccg gtacagcgcc tggtcaatca gagccactgc aacaacaacg tgttgaagct
     3181 gaagatcttt ctgcctttgg tcggggctac cgtattcgtg aagatcgctt tagctactct
     3241 ttcaacccaa cactttcaca gtcgctgggc ggcccggaag atttctatat gttccagctg
     3301 gggctgatgt ccagcgcccg ctactggttt accgaccacc tgctgcttga tggcggtatt
     3361 ttcaccaata tttacaataa ctacgacaag tttaagtctt cgctattacc cgcggactct
     3421 accctgcccc gcgtgcgcac gcatatccgt gattacgttc gcaatgacgt ttatctcaac
     3481 aacttgcaag cgaactactt tgccgactta ggcaacggtt tctatggcca ggtgtatggc
     3541 ggttatctgg aaacgatgta tgccggtgtc ggttccgagc tgctttatcg cccgctagat
     3601 gccagctggg cgctgggtgt ggacgttaac tacgtgaagc agcgtgactg ggacaacatg
     3661 atgcgcttca ccgattattc tacgccaaca ggtttcgtga cggcttactg gaacccgccg
     3721 acgctcaatg gcgtactgat gaaacttagc gttgggcaat atctggcaaa agataaaggt
     3781 gcaacgatcg acgtcgccaa acgctttgac agcggcgtgg cagtaggggt atgggcggca
     3841 atcagtaacg tatcaaaaga tgactacggc gaaggcggct ttagtaaagg tttttatatc
     3901 tcgattccgt tcgacttgat gaccattgga cctaaccgca accgcgcggt ggtttcgtgg
     3961 acaccattga cgcgtgatgg tggacaaatg ctgtcacgca aataccagct ctatccaatg
     4021 acggcagagc gagaagtacc ggttggacaa taacctgacg taattcataa cattacaatg
     4081 actgataaat ataaaccaat gaaaaagaac atttttaagt tttcggtact gacattggct
     4141 gttctgtctt tgacagcatg tacccttgtt cctggtcaga atttatcgac cagtaacaag
     4201 gatgtcattg aactacctga caatcagtat gacctggaca aaatggtcaa tatctatccc
     4261 gtcacgccag gattgattga tcagctgcgc gccaaaccga ttatgtcgca ggctaacccg
     4321 gagctggagc agcaaatcgc gaactatgaa tatcgtatcg gcattggtga tgtgttgatg
     4381 gttacggtct gggatcaccc ggaactaacc acacccgcag ggcaataccg tagcgccagt
     4441 gataccggca actgggttaa cgctgatggt gcaattttct acccctatat tggtcgcctg
     4501 aaagtggccg ggaaaacgct tacccaggta cgcaacgaga ttacagcgcg tctggattca
     4561 gtcattgaaa gcccgcaggt cgatgtcagc gtcgcggcgt tccgctcgca aaaagcgtac
     4621 gtcaccggtg aagtcagcaa atcgggtcag cagccgatta ccaatattcc tctgaccatc
     4681 atggatgcga ttaacgccgc gggggggctg accgcagatg cagactggcg caacgtggtg
     4741 ctcacccaga atggcgtaaa gaccaaagtg aacctctatg cgctgatgca gcgtggcgac
     4801 ttgcgccaga acaaactgct gcacccgggc gatattttat ttattccgcg caacgatgat
     4861 ctgaaagtct tcgtgatggg tgaagtgggt aaacagagca cgctgaaaat ggaccgcagc
     4921 ggtatgaccc tggcggaagc gctgggtaac gctgagggta tgaatcagga tgtcgctgat
     4981 gcgacgggga tttttgttat tcgtgcaacg caaaacaagc agaatggcaa aatcgccaat
     5041 atttatcagc tgaatgcgaa ggacgcatcg gcaatgatac tcggaacgga attccagctt
     5101 gaaccttacg acattgttta tgtcactact gcgccgctgg ctcgttggaa ccgtgtgatt
     5161 tctctactgg taccgacgat ttccggggtg catgacctga cagagacttc acggtggatt
     5221 caaacatggc ccaactaaaa tttaactcaa tcctggtggt ttgtaccggc aacatttgcc
     5281 gttcgcccat tggcgagcgc ttgctgcgta aacgtctgcc aggcgtaaaa gtcaaatcgg
     5341 caggtgttca tggtctggta aagcaccctg cagatgcgac agcggcagat gttgccgcta
     5401 atcatggcgt atctcttgaa ggacatgccg gacgtaagct cactgctgag atggcacgaa
     5461 attacgatct gattctggca atggagtcgg aacatattgc tcaggttacg gcaatcgctc
     5521 ccgaggtccg cgggaagaca atgctgtttg ggcaatggct ggaacagaaa gagatcccgg
     5581 atccctatcg taaaagtcag gacgcatttg agcatgtcta cggtatgttg gagcgcgcca
     5641 gtcaggaatg ggcgaagcgc ctcagccggt aatttgagtt tataaaatac gatgacaact
     5701 aaaaatatga atacgccacc aggcagcact caggaaaatg agatcgatct gcttcgtctg
     5761 gtcggcgagt tatgggatca ccgtaagttt attgtcagcg tgaccgcgtt gttcacgctg
     5821 atcgctgtcg cttactcgct gttaagcacg ccaatttatc aggcagatac tctggtccag
     5881 gttgagcaga aacagggcaa cgccattctc agcggcctga gcgatatgat ccctaactca
     5941 tcgcccgagt cagcaccgga gatccaactg ctgcaatcgc gcatgattct cggtaaaacc
     6001 attgctgaac tgaatctgcg cgacatggtt gagcagaagt attttccgat tgtgggtcgc
     6061 ggctgggcga gattaaccaa agagaaacca ggtgagctgg cgatcagctg gatgcacatc
     6121 ccacaactga atggtcagga tcagcaactg acactcacag ttggggaaaa cggccactat
     6181 acgctggaag gtgaagggtt caccgtcaat ggtatggtcg gccagcgtct ggaaaaagac
     6241 ggcgttgcgc tgactatcgc ggacattaag gccaaaccag gaacgcagtt tgtcctgagc
     6301 cagcgtaccg aactggaagc gattaacgcg ttgcaggaaa cctttaccgt tagcgaacgc
     6361 agtaaagaaa gcgggatgct ggaacttacc atgaccggtg atgatcccca gttgattact
     6421 cgtattctga acagcatcgc taacaactat ttgcaacaga atatcgctcg ccaggcggcg
     6481 caggattcac aaagccttga attcttacag cgccagttgc cagaagtgcg cagcgagctg
     6541 gaccaggcgg aagaaaaact caacgtttat cgccaacagc gcgattcggt tgaccttaac
     6601 ctggaagcca aagccgttct tgagcagatt gtgaacgttg ataatcaact caatgagctg
     6661 actttccgcg aggcagagat ctcccagctg tataagaaag atcacccaac ttatcgtgcg
     6721 ctgctggaaa aacgccagac actggagcaa gaacgcaaac gcctgaataa gcgggtatcg
     6781 gcaatgcctt ccacccaaca ggaagtgttg cgtttaagtc gtgacgtaga agcgggccgt
     6841 gcggtatatc tgcaattact taaccgccag caggagttga gtatttcgaa atccagtgcg
     6901 attggtaacg tgcggattat cgacccggca gtcactcagc cgcagccagt gaaaccgaaa
     6961 aaagcgttga atgtggtgct tggttttatt cttggcctgt ttatttcggt gggtgccgtg
     7021 ctggcgcgtg cgatgttgcg tcgtggtgta gaagccccgg aacaactgga agagcacggc
     7081 atcagcgttt atgccactat cccaatgtcc gagtggctgg ataaacgtac ccgtctgcgt
     7141 aagaaaaatt tattttctaa tcagcagcgc catcgtacta aaaatatccc cttcctggcg
     7201 gtggataacc cggcggattc tgctgtggag gccgtacgtg cgctacgaac cagtctgcac
     7261 ttcgctatga tggagacaga gaataacatt ctgatgatca ccggtgcgac gccagacagt
     7321 ggtaaaacgt tcgtcagttc aactctggca gcggtgatcg cccagtccga tcaaaaagtg
     7381 ttatttattg atgccgactt acgccgtggt tattcgcata acctgtttac cgtgagtaat
     7441 gaacatggct tgtcggaata tctggcaggt aaagatgagc tcaacaaagt gatccagcat
     7501 tttggcaaag gaggctttga tgtgattact cgcggtcagg tgccacctaa cccgtctgaa
     7561 ctgctgatgc gcgatcggat gcgtcaatta ctggaatggg cgaacgacca ttacgatctg
     7621 gtgattgtcg atacgccgcc gatgctggcg gtgagcgatg ctgcggtcgt gggacgctct
     7681 gtcggcacca gcctgctggt tgcgcgtttt ggcttgaaca ccgccaaaga ggtgagcttg
     7741 tcaatgcagc gtctggaaca ggctggcgtt aatattaaag gcgcaatcct caatggtgtg
     7801 attaaacgcg ccagcaccgc ttacagttac ggctataact attacggtta tagttactcc
     7861 gagaaagagt aaaactgaag gtaaggtggc cggatggggc gtgaacgcct tttccggtct
     7921 acgcattaga cagttcttcg tttgtcatca gcctcagagc attcagataa ctgaatgctc
     7981 ttttttatgc attacaaact gcacgccggt atgcgtgctt cattcacgat ttgcgtaaaa
     8041 cctgccaacg aacacatgcc ctgcgcattt cgctcttcac atcctgccag ggtcagtttc
     8101 acctctccgg gcggcgtatt taatgacagc ggcgttttat cacgcatctg ctgtaaagtc
     8161 tggaagacca gcgaaacctg aatccactgg ctgttatcgc ttagccgacg ccagcgttca
     8221 aacaccagtt caccacctgg cggtgtgtta tccggctgac ctggaagcgt ccagttgagc
     8281 tccagtgcgc cgccgagatt tgccagatta gtatcgtgtc cggcaataaa cagcactgaa
     8341 gtgggtaatg tcacaccata cgcctgtttt tgcggtggat ggggcgtcaa cgctgccatg
     8401 atcaaatcca ataacggggt ggcgcgactg cgggcaacct ctggcgtgcg ttgtagtaaa
     8461 taaaattgcg cgttatgcaa acttagcaag gtgttccact ggtgtgaatc agtgatcctt
     8521 ccccaccccg gctccggcat tccttgtgcc tgttgcagga gaaatatctc cgtcagcatt
     8581 gatgcgaggc ttaccgcacc ggttaatgag acattgtcgg cgctcacctt gagttccgat
     8641 ggtaatgcct gcgttaatga acagctttcg tcctgtttct cacggttaag gcacaagttt
     8701 gattgcgaaa aattaagcac ccgttccagt tcgcgaaacg ccgtttgccg atgcccggta
     8761 aagtcagcaa ttgaccctcc tgccctgctg aggatcgcgt cagtcacgtt cgcattatcc
     8821 agttggcaaa cgccagtttt taggggatta aataacggat cgggactgga cgtatctgcc
     8881 tgggtatgta cggttattgc acagtcaggt gccagcccgg cggcgaaggc ttcgcctgtt
     8941 ttacgggtac gctcgtcgac atcagcaata atcgcgacct gaccaggctg cgggcagccc
     9001 tttttcgcca gcaatccgtc agccaccaga cactggcgtt ggtaatgtcc aagataagcg
     9061 attagctcac caccgcgcgg tgtcagccaa cccagtttta ccggccaggt tggccatgcg
     9121 tctggggtga catcctgcat cagttgcgtg gccttggttg gagcacgcac accatgacga
     9181 ctgacaatca ccacactttc cagcttcagc tccggctcac tctgagcgaa tgcagattgc
     9241 ggggttaacg gaatcagaag agataaaaat gggattaaga tcgctttcat cgatatgttt
     9301 ccgcttttca tatctgacat tattgattgc ctgatgcgat gctaatgcac cacatcaggc
     9361 ttacgttcgt taacttttca gacgcgggtc cagccatacc aacacaaggg tggagagcga
     9421 acacatcaac aaaatgccga cgaaccaaag taaataccac attgttcctg ctccttagta
     9481 cagctcgttt tcgttacggc ggagagtttc tgtcgtcatg cgtccccaca ttttgtagta
     9541 gctccagaga gtgtagagca acacaatagg caaaaatatc agcacgatta ccaacataat
     9601 gctcagcgtc agctgactgg aagtgctgtc ccacaacgtc aggctggaga tcggactcac
     9661 gcttgacggc atgacaaagg ggaacagcgt gatgcctgcc gtgaaaatca cgccaaattg
     9721 catcaatgac gccatcaaaa atccccaacc cgggcgacca cgataaatcg ccatcaccgt
     9781 cagcaatggg cagaagaacc ccagcagcgg gaagatccac aaaacgggcg attcgacgaa
     9841 attattcatc caggcaccag gtagcactgc caccagtttc attaacggat tggaaggacc
     9901 gttagcatcc tgggcgagca gtacaaagcc atcaatacca acccacagcc agtaacccgc
     9961 cagcaaaaag cacagcatca ccagtagtgc ggcgcgtttg gtcgccagct gtgaacgcag
    10021 atgaatcaca ccaacagttt tcagttgtaa ccagacgcca ccttgcaaaa tcaccatccc
    10081 aagactgagc aatccacaca ataaagggaa tggcgtcagc agttgccaga agctgccgag
    10141 atattccacg cgtaattgcg gtgtgaaagc aaacggcacg ccgagcagca agttgccgaa
    10201 tgcaataccg aagactaccg gcggcaccag actgccgatg accagtccgg catcccacat
    10261 tttacgccag cgtgcatcgg cgatttttcc gcgataatca aaggccagcg ggcggaagaa
    10321 cagcgagcac agcaccagaa tcatcgccac ataaaagccg gaaaacgccg ctgcatacac
    10381 tctgggccag gcggcaaata atgccccacc tgcgaggatc aaccagacct gattgccttc
    10441 ccagtgcgcg ccaacgctgt ttatcactat ccgacgttca tcatcattac gcgccaccag
    10501 cggcagcaga cagccgatcc ccatgtcaaa tccgtcggag atcataaaga ccaccaggat
    10561 cacgccaatc agcagccacc agatgaagcg caatgtttca taatcaaaca tgattttctc
    10621 ctttacccct gttgctgcgt cggttgttca ctctgcatcg cgctcggccc cagacgggca
    10681 tatttctgca tcaggtagac ttcggcgatt aagaacaggg tgtaaagccc tacgatcatg
    10741 atcagtgaga aagccagttg tcctgtggtt aaggcggagt gcgcggagta tgtcggtaag
    10801 atgtcctgta tcgcccacgg ctgacgacca aactcggtca taaaccaccc ggcttcaatc
    10861 gcaatccacg gcagcggcaa actccagagc gccattttca gcacccagcg atgctggtca
    10921 attttgccac gcagcgtctg gacaagcgca atcagcatca ccagtagcag cagagaacca
    10981 cagcccacca tgatgcggaa actccagaat accggcgcaa cctgaggtat cgcgccacgc
    11041 atcgccgcct ggtactgtgc agctgtgaca tggttcatat ccggcgcata gcgggagagc
    11101 aacatgccgt aaccaaggtc gccttccagc tcccgaaatg cctgcaacac atgcggctca
    11161 cgattgcctt gcgatatttc ctgcattaac agccaggcca tacgtccgcg ttgcaagcgt
    11221 gggtaggttt cagccatcaa attcttcaga cccggcacgg gtttatctaa tgagtgagtg
    11281 gcgaggatcc ctagcagcgc gggaattttg atagcaaagg cgttacgctc ttgatcctgt
    11341 tccggccagg caaccacatg gaacggtgca ggtgcaggtt ccgtttgcca ctccccttcc
    11401 atcgccgcca gttttaccgg ttgtacttgc gcgacttcat acgcagaact gtctccgagt
    11461 tgcagcgtgc cgataatcgc cagagtaccg aagacggaac cgatggcaaa cgagcgtaat
    11521 gcaacatcgc gctcccgtcc gcgcagtaaa taccaggcgc tgatcgccat aataaacatg
    11581 gccccggtca cgtagcccgc cattacagtg tgcacaaatt tcacctggct gaccgggtta
    11641 aagaccaact cgctgaaact ggtcatctcc atacgcaggg tgtcgatatc aaaatgcgca
    11701 ccggtcgggt attgcatcca accgttggca ttcaatatcc acaatgcaga gagatttgaa
    11761 ccgaacgcca ccagccacgt caccagcagg tgctggtatt tattcagccg ctgccagccg
    11821 aagaagaaca gcccgacaaa ggtggattcg aggaagaagg ccattaatgc ttccatcgcc
    11881 agcggtgcgc caaaaatatc gcccacatag ttggaataga atgaccagtt agtaccaaac
    11941 tgaaactcca tcgtcaggcc ggtagccacg ccaagagcaa agttgatacc gaagagctta
    12001 ccccagaagc gcgtcatatc gcggtagatt gttttgccgg tgaccacgta aatggtttcc
    12061 atgatagcca gcaaaaaaat cagccccagg gtaaggggta caaataaaaa gtgatacagc
    12121 gcggtcagag caaactgcca gcgcgataaa tcaatgacat cccacatgcg cactcctgta
    12181 ggcgaagaga gaaaaataac ccgttaatgg tagatctccc tctttatcct gaaactctac
    12241 gaccagaggt attcgccgat tgcgatattc ataagcctga aagcgatact taccgtcttt
    12301 ttacgtcggt gcaccttccg ccagccactg gcatacctcg ctaagccgct gcgcagagtc
    12361 gaccaaatct tctggcgctg ccagcaccac ttccggtatt gggcagattt cataactttc
    12421 cagtaacggg ccttttaagg tgtccgtgca gcgtaaatgc cagacatggc gtaaccccgt
    12481 ggcgttgata tagctctcgc catagccaat ggtgcgaatc tgaatatttc ccggcccaca
    12541 gagacgtgag agaaaaagcc gatcagcctc gctgatgggt aattgcgtca gattaatgct
    12601 gtgcggctgc gcgtcggggt tacgtacatg cgccagtagc tcatgcgcca acggtaggcc
    12661 attcatcagg ccatcaatgg gcggcggtaa cagcgaatct gtcggcaaga cgttttgcgt
    12721 tgccgcctgc cacaatgcca gcggcgcgca gccagcctcc agtttgtcct ccagcaactt
    12781 gtcgccgcga cgtctgcgca cccgccataa tccgcagaag atcgcctcct gtatttcact
    12841 ttcactgtcg tcagcctgct gtatgcgcac tgacacttcc ccttccccga gaagcgtatt
    12901 gataaagtga cgatcgtcgg cgttgagatt taacagatcg actgccagca cctcgcccaa
    12961 cggtggctgg cgttcgctta tttgttgttg taactcgttt aacagcgcaa tcacctgcgg
    13021 gctgtgagca cattgctcca gggccgccat actcggttca tcattcacct gacaggtgat
    13081 cggcagtgga ttcatgctga aactgtcatc gttcggttgc gttcctggcc ccaacagatg
    13141 gaaaaaagtt tcgctcatga ggcacgctcc tgttgtggtt cgacaagccc acgcatcagg
    13201 tttatcagtt ccgcccacgg gtgaatacca ttcagcacgc cgcgatagct accaccggta
    13261 aacaccagtg tggcgggaaa acgaaatacg ccaaaacgat caccgatggc ttcgctctgc
    13321 tcaagatcag caatcgctac ctgccatgta tagtcgggaa actcgcgcag taattcgcca
    13381 atcattaccg ggttatcgct gacttctggc gtgcgtttcg ggtcactact taataacacc
    13441 acgccgtctg gcgcttgcgt aagccagtcg tcaagacggc attcactgac cggcgtccag
    13501 ccgcgcgcca gcattcgttg ccacaacgca ttaaatggtg tgtcgttgct catccctgtt
    13561 cctccagcct catcctgtac tgacgcaagc gaacgccttc gtaattttcc atcgacagac
    13621 agtcataatt gagacagcgc gattccttag ccggttgcgg cacaattccc cacgcagcca
    13681 gctgcgccag cgccgattgt tccgcagcgg gcagttgctc ccgcgccagt tcgctcaggc
    13741 taccgccgta gtcgtcgagc attgcgggtt gcagaccgac gagggcaata tgtgctggca
    13801 aatgaccacg tatatccgcc agcgccagca cttcggagaa gctgttctgg tgcaggctca
    13861 ttttcttcgc gctgagataa gccggaatgc gttctccggc ataggttcgc agcgttcctg
    13921 gttccagccc gtagtcaatg gcatcgagaa tcaacaagtg actggcgctt tcgacatacc
    13981 ccagcaagtt cagtccctga gtaccgccat cgacaatctc cacatactcg ggccagtggt
    14041 aatgggcata cagccgttcc gccacccgca cgccgaagcc ttcatcggcc cacagcaggt
    14101 tgcccagccc cattaccacc acgcgttgct cgctcatgaa cgctccttgt tacttatttt
    14161 gccaaatttg tggctacggt agccgttgac catggtggag atcaccgtgt cgtcggacat
    14221 gatatcttca cgcagcgcca tgtagacatg accgatcaca aacgcgccaa tcagccacat
    14281 ccccagccga tgccagctgt gaacgtccat tgagttgccg cccgtccagt agaaaaattc
    14341 caccacataa cggaacggcg caaaaatagc gtactggctg tgttcgctgt acagcgcaaa
    14401 gccggtgatg atcataaaaa ctgacatcag gaaatagccg aacatcgccg cctgggcgat
    14461 ggggttatga cctatatcgg cactcggacg ttttgccaga aacagatacc agcggatttc
    14521 ataccacacg ccctgccacc agcttttacg ccatactggc acgataaaca gctcgcgtga
    14581 gtagcgattg ccgacaaaag cccagtagat ccgcatcagc aagaccacgg taaaaaccat
    14641 cccggcgctg aagtgaatta acctgatgta gcccatatag aacagatacg tcgcctcgcc
    14701 gctgacggaa ggtagcggct tgccgataaa gtaaccggtg accatcaaca ccgccatgca
    14761 taacaccgtc agccagtgcc agatacgcac tggcgcttca aagacatagt ggctgacaac
    14821 gttgtcgctt ttctgttgca tgatgattct ccttcgctgt taacgcacct gcacggagat
    14881 cagctcgcta ccgtcgtcgc tcagcacgtg tgttgaacag gcgaggcacg gatcaaagct
    14941 gtgcagagta cgcaggatct ccagcggttg ctcggggatc gccattttgg tgttcatcag
    15001 cgccgcttca taagcgccaa tttgcccttt aggatcgcgc gggctggcgt tccaggtggt
    15061 cggcaccacg cactggtaga gatcaatctt gccatcgcga atggcggccc agtggcctaa
    15121 cgctccgcgc ggcgcttcgg taaagccgac accacggcac tctgtcggcc aggttgctgg
    15181 ttcccacttc tcagtggagg cggtggcgag attgccgttt ttgagattgg tcatcagctt
    15241 gtcgaagaaa tattgcaact tgcctgcggc ccactgcgct tcgtgcgcgc gacacaaaat
    15301 gcggcctaat gttgactgga tgccggaaag cggcaggttc aacgccgaca tcatgcgatc
    15361 gaccgactca acggtcgccg catcgccttt gtgataagcg attaacgtgc gtgccagcgg
    15421 ccccacttcc atcgcattac cgcgccagcg tggcgctttg atccacgagt agcgttcctg
    15481 ttcattcagc tgctgaatgt tggtatcgct gcctttgaca tcgccggggt tgtaccacgg
    15541 gtcggtaatg ccatcgaacg gatgacgccc gacctgatcg ttgggataac gataccaggc
    15601 gtgatcgaca aactcctgca cctgctgcgg atcaaccaaa tccactggca gcacattgtt
    15661 gaagtcgccg ttaatcaccg cgccgccagg catcagtaga cttttctcgc caaagtcgtt
    15721 ggcgatgtcc gggaacgcgc cgtaactgag aacgcattta tccgaaaggc cagtgccgat
    15781 ttcgctccac ggcttgttga actgaccgat ggctaaggcg tcggggatca tcacgttgtt
    15841 aatgaaatcc gccgtacggg taatgattga ctgcaccagg ttcaggcgtt ccatattgac
    15901 cgccccgacc gcgccgcttt cgtcaatgtt aatggcacaa ggcatcccgc cgacaatcca
    15961 gtttggatgc gggtttttac cgccaaagac cgcgtggatt ttgacaattt cacgttggaa
    16021 atcgagagct tcaagatagt gggcaaagcc catcaggttg gcttctggcg gcagtttgta
    16081 ctgcgggtgc ccccagtagc cattgcggaa gatccccaac tgcccgcctt caacgaattt
    16141 cttcaggcgg ttttgtacgt cgaagaaata gccaggggat gatttcggcc atgaggagag
    16201 gctttgcgcc agttcggagg ttttccgcgg gtcggctttc agcgcatcta acacatcgat
    16261 ccagtccatc ccggcaagct gatagaagtg caccagatga tcgtggcacc agagcgttgc
    16321 cagcataatg ttgcggatga tattggcgtt gtccggcact ttaataccga tggcatcttc
    16381 gatggcgtaa accgaagcca gagcgtgtac gccagtacag acgccgcaga tacgttcaac
    16441 gaacgcccac gcatcgcgcg ggtcgcgccc ttgcaggatg atctccagcc cacgaaacat
    16501 ggtgccgcag gagacggcat tggtgatcac attctgatcg ttaatattca cttcgcagcg
    16561 catgtggcct tcaatgcgcg taatcgggtc gaccaccagg cggcgtccgg cattattgat
    16621 ggtgtatccc tgagtttcgt actgagtgct catgcctgtt tatcctcatt gcctggctga
    16681 tgttcggttt ctgtaggttg ctggttatgg cgtctgcgct ggtcaacggc gctggcgact
    16741 gcgtgcacac caacagccgc tgccaccacg ccaagtgcgg ttaaaccgac ggtatcggcg
    16801 gtggaatggg tacccatttg cggaatatcg accacgcggc tgtagaacga accgcgatcc
    16861 cagaaaccat tttccgcaca acccaggcag ccgtgacctg actggatcgg gaaggaaacg
    16921 ccatcgttcc agcgtgtgga ggaacaggcg ttataggtgg taggcccttt gcagcccatt
    16981 ttgtacaggc agtaaccttt gcgggcagcg tcatcatccc aactctggac gaactctccg
    17041 gcgtcgaagt gagcgcggcg ataacattta tcgtggatac gctgaccata aaacatcagt
    17101 ggacggccca ttttgtcgac atctggcaag cgatcaaagg tcaccatgta agtaatgatg
    17161 gcgctcatca catccgggat cggcgggcag ccaggtactt tgataatggg tttgtcggtg
    17221 atgactttgt cgataggcgt tgcctgcgtc ggattgggtc gcgcggcctg cacgcagccc
    17281 caggacgcgc aggttcccca ggcgataatc gcactggctc cggcagcggc acgtttgagt
    17341 ttctcaataa acggtcgtcc gctgctgata cagaacatcc cctgctcgcc cagcggcgga
    17401 ttaccttcta ctgcgaggat atatttgcca ttgtattgcg tgatgatgtc ttcaaagact
    17461 tcttccgcct gggttccggc ggcagccatc aaagtatcgt cataatcgag ggaaatcagg
    17521 gaaaggatga cgtccttcgc cagtgggtga gcggagcgga taaaagattc ggtacagcag
    17581 gtgcattcca gaccgtggat ccataccacc ggaatgcgcg gtttgttctc cagcgcccag
    17641 gcaatctttg gtgccattcc cgcgcctaat cccagcgacg tggcagccag actacaatat
    17701 ttgagaaagc tgcgccgggt aacgccctga cgccgcatgg cctggtaaaa tgtttcctcg
    17761 ttattcatat cgcacgtctc ttctccttgc gacaccggca ggacaccggg cgcttatggt
    17821 cggcaagcga cgtccctgta gcaaggcaaa ccatcgcttc acgtgagatg ctgaaaacga
    17881 aagctcatcc ttctgcactg gcgcacgtcg ccagaaagta atgttaataa agcgtagtga
    17941 aacttttgca caaaacaata caaactgtgt ggatttatct tttagcgata aaaatggacc
    18001 tatttttctt tttgccgggc agtggggatg tttagccggt tgctaaacga gtaaaagaga
    18061 aggaattcat ggtgatgaaa tgatcaatct gctgttattt catgctatca atgcactttt
    18121 ggctgttttt caggcaaaca aacaaattag gggtttacac gccgcatcgg gatgtttata
    18181 gtgcgcgtca ttccggaagt gtggccgagc ggttgaaggc accggtcttg aaaaccggcg
    18241 acccgaaagg gttccagagt tcgaatctct gcgcttccgc caaacaatca aggggttacc
    18301 aaatggtagc ccctttcttt ttttgctctt agtatacact tggtatacta attggtatat
    18361 ttagctcaat acacactagg tacattgttc tctttacgat ttatcgctat accgatgaat
    18421 tatgaataac cccaatccat gcatgacgtg tggtgcctgt tgtgcatttt tccgcgtctc
    18481 tttttactgg gcagaagctg acgatgctgg cggaaaggtt ccggtcagtc tcactgagca
    18541 aatatccccc tttcatcgct gtatgcgcgg caccagtcag aaaaatcccc gatgtgtcgc
    18601 tctggcggga actccgggta aaaacgcata ttgctctgtt tacaagaacc gtccgtccac
    18661 atgccgggaa ttcgcaatgt ccggcgaaaa tggcatcgcg aatgaagcat gtaaccgcgc
    18721 acgggcaaaa tacg
//
LOCUS       DAZGAP010000105        18488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGAP010000105 DAZGAP010000000
VERSION     DAZGAP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.105"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..1066
                     /gene="phoE"
                     /locus_tag="ACHLAM_003135"
     CDS             11..1066
                     /gene="phoE"
                     /locus_tag="ACHLAM_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO7854624.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1105..1506)
                     /gene="crl"
                     /locus_tag="ACHLAM_003136"
     CDS             complement(1105..1506)
                     /gene="crl"
                     /locus_tag="ACHLAM_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7854625.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(1564..2808)
                     /gene="frsA"
                     /locus_tag="ACHLAM_003137"
     CDS             complement(1564..2808)
                     /gene="frsA"
                     /locus_tag="ACHLAM_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7854626.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2900..3358)
                     /gene="gpt"
                     /locus_tag="ACHLAM_003138"
     CDS             complement(2900..3358)
                     /gene="gpt"
                     /locus_tag="ACHLAM_003138"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7854627.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            3619..5076
                     /gene="pepD"
                     /locus_tag="ACHLAM_003139"
     CDS             3619..5076
                     /gene="pepD"
                     /locus_tag="ACHLAM_003139"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7854628.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5133..5485)
                     /gene="prfH"
                     /locus_tag="ACHLAM_003140"
                     /pseudo
     CDS             complement(5133..5485)
                     /gene="prfH"
                     /locus_tag="ACHLAM_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5481..5687)
                     /locus_tag="ACHLAM_003141"
                     /pseudo
     CDS             complement(5481..5687)
                     /locus_tag="ACHLAM_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6005..6457)
                     /gene="yafP"
                     /locus_tag="ACHLAM_003142"
     CDS             complement(6005..6457)
                     /gene="yafP"
                     /locus_tag="ACHLAM_003142"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7854629.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFSMRYKPQH"
     gene            complement(6454..7509)
                     /gene="dinB"
                     /locus_tag="ACHLAM_003143"
     CDS             complement(6454..7509)
                     /gene="dinB"
                     /locus_tag="ACHLAM_003143"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO7854630.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7580..8365)
                     /gene="lafU"
                     /locus_tag="ACHLAM_003144"
     CDS             complement(7580..8365)
                     /gene="lafU"
                     /locus_tag="ACHLAM_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO7854631.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            8337..10049
                     /locus_tag="ACHLAM_003145"
                     /pseudo
     CDS             8337..10049
                     /locus_tag="ACHLAM_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(10267..10764)
                     /gene="rayT"
                     /locus_tag="ACHLAM_003146"
     CDS             complement(10267..10764)
                     /gene="rayT"
                     /locus_tag="ACHLAM_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO7854632.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNMWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(10941..11690)
                     /gene="yafL"
                     /locus_tag="ACHLAM_003147"
     CDS             complement(10941..11690)
                     /gene="yafL"
                     /locus_tag="ACHLAM_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7854633.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            12002..12742
                     /gene="dpaA"
                     /locus_tag="ACHLAM_003148"
     CDS             12002..12742
                     /gene="dpaA"
                     /locus_tag="ACHLAM_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO7854634.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(12713..13480)
                     /gene="yafJ"
                     /locus_tag="ACHLAM_003149"
     CDS             complement(12713..13480)
                     /gene="yafJ"
                     /locus_tag="ACHLAM_003149"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO7854635.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13596..14174)
                     /gene="lpcA"
                     /locus_tag="ACHLAM_003150"
     CDS             complement(13596..14174)
                     /gene="lpcA"
                     /locus_tag="ACHLAM_003150"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO7854636.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            14414..16858
                     /gene="fadE"
                     /locus_tag="ACHLAM_003151"
     CDS             14414..16858
                     /gene="fadE"
                     /locus_tag="ACHLAM_003151"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO7854637.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(16901..17347)
                     /gene="ivy"
                     /locus_tag="ACHLAM_003152"
     CDS             complement(16901..17347)
                     /gene="ivy"
                     /locus_tag="ACHLAM_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO7854638.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            17528..18298
                     /gene="yafV"
                     /locus_tag="ACHLAM_003153"
     CDS             17528..18298
                     /gene="yafV"
                     /locus_tag="ACHLAM_003153"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO7854639.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
     gene            complement(18368..>18488)
                     /locus_tag="ACHLAM_003154"
     CDS             complement(18368..>18488)
                     /locus_tag="ACHLAM_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO7854640.1"
                     /translation="AINILTNDNVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         4481 a         4742 c         4811 g         4454 t
ORIGIN      
        1 aggaatgaaa atgaaaaaga gcactctggc attagtggtg atgggcattg tggcatctgt
       61 atccgtacag gccgcagaaa tatataacaa agacggtaat aaactggatg tctatggcaa
      121 agttaaagcc atgcattata tgagtgataa tgacagtaaa gatggcgacc agagttatat
      181 ccgttttggt tttaaaggcg aaacacaaat taacgatcaa ttgaccggtt atggtcgctg
      241 ggaagcagag tttgccggta ataaagcaga gagtgatact gcacagcaaa aaacacgtct
      301 cgcttttgcc gggttgaaat ataaagattt gggttctttc gattatggtc gtaacctggg
      361 cgcgttgtat gacgtggaag cctggaccga tatgttcccg gaatttggtg gcgactcctc
      421 ggcgcagacc gacaacttta tgaccaaacg cgccagcggt ctggcgacgt atcggaacac
      481 cgacttcttc ggcgttatcg atggcctgaa cttaaccctg caatatcaag ggaaaaacga
      541 aaaccgcgac gttaaaaagc aaaacggcga tggcttcggc acgtcattga catatgactt
      601 tggtggcagc gatttcgcca tcagtggagc ctataccaac tctgatcgca ccaacgagca
      661 gaacctgcaa agccgtggca caggcaagcg tgcagaagca tgggcaacag gtctgaaata
      721 cgatgccaat aatatttatc tggcaacttt ctattctgaa acacgcaaaa tgacgccaat
      781 aactggcggc tttgccaata agacgcagaa ctttgaagcg gtcgctcaat accagtttga
      841 ctttggtctg cgtccatcgc tgggttatgt cttatcgaaa gggaaagata ttgagggcat
      901 cggtgatgaa gatctggtca attatgtcga tgttggtgct acgtattatt tcaacaaaaa
      961 tatgtcagcg tttgttgatt ataaaatcaa ccaactggaa agcgataaca aattaaatat
     1021 taataatgat gatattgtcg cggttgggat gacgtatcag ttttaatgaa tattgccgga
     1081 tgtgatgcat ccggcacatt tcattcacgc cgttaacttc accggttcat cacgaaaatc
     1141 atctgccggt tccagcttca gattcagcgt cgccagcagc tcacgcagct tctcgtgaaa
     1201 ttcacgcagg gtgtgctcca gtcgttcaac cacttcggca tcttttatct gaacactcgt
     1261 ccagtcgcct gctttatcga acagaccaaa ctggtaactg taggtaaaac gggattcctg
     1321 cgcttccagt tccatccacc agccccagaa ctcacgcact tccggtgccg gtttcacgtt
     1381 gacgcataca gccagacaat cgaaaaagaa tcgattatct tcgcacttac cttcacgaat
     1441 atacgggcct agtgcggtaa attttttgat caatctgctc ttcgggtgtc cactcggtaa
     1501 cgtcattgca atctcctgtt gtgatgcaac tgttttacca aattggtaaa atttagcaaa
     1561 tttttaacac aaacgttttt cgatccagtc tgtgatttcc tgaaggcctt tgtcaaaatt
     1621 ccgatacacc gggttaaatg ggatctctaa taatttaccg tcagcagatg acgacgtgat
     1681 taagcgtgag tcctcttccg ggctgaacgg atcgttcttc cagtagcctg ataacattgg
     1741 cgttgggcag cgacgtccca gcaatccttg cacttttaat gaatagcgat tcaactccac
     1801 tcgcaacgct tcatcggaag catcatgcat ccctaaacga ctggccagaa cgtcaagata
     1861 catttccggc acctgttgct ggcacttaaa atcactcaac agggtatgaa ctaccggacc
     1921 aagacaggca accactttca gacgcggcga ttcaaggtat gccagacgca cggcaacgtt
     1981 agcgccgaaa cggaaaccaa aggccgcgac gcgagtgtga tccacccacg gtacgttagg
     2041 cagcgccttt aaaacgtgct gatgcaacag gctggagtcc tgggtgagct tccattttga
     2101 agaaaagccc accgacggca tatcaatagt cagcatagca atgccgcgcg gcgcaaaata
     2161 acgttcatac aggctgtaat agtccgtctg catcgcatcc agaccaccac acattaatac
     2221 tgtcgggaac gggccatcgc ctttcggcat atgcaaaaag ccggtgatgg gcgcaccgcc
     2281 gggtacggta aactccatct gccgcatcgt gcccggcaga cgctgagcgg cctcttcata
     2341 ggcgcggttt gacaaagcct gcgcttgctc ggccaggtca tctcctttca gatgaggata
     2401 ggcagcaatg ttgtacaacg tagccgcatg cagccagtat cgaccgctga gttgcggatc
     2461 ttcttccgca caggcttttt gttgccacac catcgcctgg gtagcccact cataaatcca
     2521 gttgccgccg cggtagccaa tcaccgtatc gtataaatca tcgtcggtac gttcggcatc
     2581 gctcatcaca atacgcgcct ggacgtcgag gatttcacgg gggtcaatgc cgcgccagat
     2641 ccacatcaga cggttaatca tgcgatacca gtgagggatg gttttaccat caagggccga
     2701 ctgcacaggc agttgtgcgc cgtgactaaa gcggcggact agcgtcgagg tttcaggatg
     2761 tttaaagcgg ggtttgaaca gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc
     2821 cagaatactt actggaaact attgtaaccc gcctgaagtt aaaaagaaca acgcccggca
     2881 gtgccaggcg ttgaaaagat tagcgaccgg agattggcgg cacgaatacg acgcccatat
     2941 cccacggctg ttcgatccag gtattttgcg ggatatcaac aacataatca tcaaccagcg
     3001 gacgaccagc cggttttgcg aagatggtga caaagtgcgc ttttggatac atttcacgaa
     3061 tcgcaaccgc agtaccaccg gtatctacca ggtcatcaat aacgatgaag ccttcgccat
     3121 cgccttctgc gcgtttcagc actttaagct cgcgctggtt gtcgtgatcg tagctggaaa
     3181 tacaaacggt atcgacatga cgaataccca gttcacgcgc cagtaacgca cccggtacca
     3241 gaccgccacg gcttacggca ataatgcctt tccattgttc agaaggcatc agtcggcttg
     3301 cgagtttacg tgcatggatc tgcaacatgt cccaggtgac gatgtatttt tcgctcatgt
     3361 gaagtgtccc agcctgttta tctacggctt aaaaagtgtt cgaggggaaa ataggttgcg
     3421 cgagattata gagatctggc gcactaaaaa ccagtatttc acatgagtcc gcgtcttttt
     3481 acgcactgcc tctccctgac gcgggataaa gtggtattct caaacatatc tcgcaagcct
     3541 gtcttgtgtt gacaacattt tctgctaacc ctgtgacctg caatactgtt ttgcgggtga
     3601 tcgacaagga gacttaacgt gtctgaactg tctcaattat ctccacagcc gctgtgggat
     3661 atttttgcca aaatctgttc tattcctcac ccgtcctatc atgaagagca actcgctgaa
     3721 tacattgttg gttgggcaaa agagaaaggt ttccatgtcg aacgcgatca ggtaggtaat
     3781 atcctgattc gtaaacctgc taccgcaggt atggaaaatc gtaaaccggt cgtcttgcag
     3841 gcccacctcg atatggtgcc gcaaaaaaat aacgacaccg tgcatgactt caccaaagat
     3901 cctatccagc cttatattga tggcgaatgg gttaaagcac gcggcaccac gctgggtgcg
     3961 gataacggca ttggtatggc ctctgcactg gcggttctgg ctgacgaaaa cgtggttcac
     4021 ggcccgctgg aagtgctgct gaccatgacc gaagaagccg gtatggacgg tgcttttggc
     4081 ttacagagca actggttgca ggctgatatt ctgattaaca ccgactccga agaagaaggt
     4141 gaaatctaca tgggttgtgc gggaggtatc gacttcacct ccaacctgca tttagatcgt
     4201 gaagcggttc cagctggctt tgaaaccttc aagttaacct taaaaggcct gaaaggcggt
     4261 cactccggcg gtgaaatcca cgttggcctg ggtaatgcca acaaactgct ggtgcgcttc
     4321 ctggcgggtc atgcagaaga actggacctg cgccttatcg atttcaacgg cggcacactg
     4381 cgtaacgcca tcccgcgtga agcctttgcg accattgctg tcgcagctga taaagtcgac
     4441 gctttgaaat ctctggtgaa tacctatcag gagatcctga aaaacgagct ggcagagaaa
     4501 gagaaaaatc tggccttgtt gctggactct gttgcgaacg ataaagctgc cctgattgcg
     4561 aaatctcgcg atacctttat tcgtctgctg aacgccaccc cgaacggtgt gatccgcaac
     4621 tccgacgtgg caaaaggtgt ggtcgaaacc tccctgaacg tcggtgtggt gaccatgact
     4681 gacaataacg tagaaattca ctgcctgatc cgttctctga tcgacagcgg taaagactac
     4741 gtggtgagca tgctggattc gctgggtaaa ctggctggcg cgaaaaccga agcgaaaggc
     4801 gcatatcctg gctggcagcc ggacgctaat tctccggtga tgcatctggt acgtgaaacc
     4861 tatcagcgcc tgttcaacaa gacgccgaac atccagatta tccacgcggg cctggaatgt
     4921 ggtctgttca aaaaaccgta tccggaaatg gacatggttt ctatcgggcc aaccatcacc
     4981 ggtccacact ctccggatga gcaagttcac atcgaaagcg taggtcatta ctggacacta
     5041 ctgactgaac tgctgaaaga aattccggcg aagtaattat ttgatttgct gccggatggc
     5101 gtttaatcgc cttccggcag tttcatcctt cattatcctt cgataaaagc catccctgtg
     5161 aacgttcgtc gcgggttgcc acgttcaatc tggtgatgga acattcgccg ctgcgatttc
     5221 agcgccgcgc tattttcctg ttgctgttgt tccagcttcc aggcaatcag caatcgcgcc
     5281 agccgcttgt tagcatgccc agaaaccagt ttttgcgccc atgatgaggc cgatacggac
     5341 tcggacaaat acactgaata gtgccgcacc aactttcgct taatgcccat gcattatcgc
     5401 catccagaga aatcagcgcc gaacgcagcg tgtcagagta gcggcccgtt tctgtttcca
     5461 gcaccgttac cgcgacgtct tgccgggtaa aaacgaccaa cagagaagaa cgctgcgcca
     5521 atcgggtagc cgcgtccggg atgtaaatct ggcatcccca caacgcgctg catgttgggt
     5581 aaatttgccg tggtgtgtaa ttgttggatc gctaaactct cgatccacag gtcatcagat
     5641 gcgatggtaa ataccgcatc ggataaggga cgaatatatt tgcccatgta tcattccatt
     5701 tatactcgtc atacttcaag ttgcatgtac tgcgaccgcg ttcgttcacc ccagtcactt
     5761 actttagtaa gctcctgggg attcactcgc ttgtcgcctt cctgcaactc gaattattta
     5821 gagtatataa atataaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat
     5881 taatcctgga aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt
     5941 gcagtttgtg gaataagaaa acgcgcacat accaggcggg tgttattttc attgcaagct
     6001 ggatttaatg ttgcggttta tatcgcatag aaaaattagt aaaccacgct cccctgcatt
     6061 caacgtgctg ctgcttaact gtctgaaaac cataacgttc aaaaaagggt tttgcggtta
     6121 tgctggcgtc caccgtaagt tcggattcag acttaatcaa aggttttaac aaggcgctgg
     6181 caaccccgcg gcgggtgtat tcagggtcaa caaataacat atcgatatag tgttcaacgc
     6241 aggtaataaa gccgaccagt tttgcattaa tgactgcaac ccgcacttgt gatttcgcga
     6301 gtttctcctt ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt tgtggtgaat
     6361 aatgctgact ggcggtcatc gtaaccgctc taaggaaaat agcgcatagt tgctgaaaat
     6421 cgccaggctg atagtctctt atttgtatgt tattcataat cccaacacca gttgtctttc
     6481 catttgcggg tcaagtaacg tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg
     6541 gcgttcatcc caggttttac gcgcagtggc gattagatca gctttattca gccgtggcca
     6601 gacgtgctcc tgagtagttt gctgaaaatc gtcgaacttt aatttcactc cctgacgggc
     6661 gatcagtaaa tcaggtttca cctttgccag acggcgttca agttcaggat acagccgctc
     6721 gataatcgct tcacattcag accagtgatg aatatcttcc gccatcgtgc gttccacgcc
     6781 gacggatttt cgcaaccgtt cactgttaac gtcgcgctcg tcaatcccct gactacgctc
     6841 ccacaaaatg cggccaaatt tgccaaagcg tttaagcaga atcaccagat cacacttttg
     6901 cacatcaccg caggtccgca gccccatcgc ttccagtttt gccgccgaga ctttgccgac
     6961 gccgggaatt tttgccagcg gtaaggtttg cagaaatgcc ggaacttctg ccggcgtaat
     7021 cacaaactga ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa actttactgg
     7081 tgccacgccc gcagatgccg tcagttgcag ctcgttgaag atcgtctggc ggatttcctg
     7141 ggcgatgagg gtcgcagaac cgtggcaatg gacgctatca gtgacatcga gataggcttc
     7201 atccagtgac aacggttcaa tacgcgaggt gtagcgcgag aagatttcac ggatatgatt
     7261 tgaggcttct ttgtaggcgt caaagcgccc cggaagcaag gtgagatgcg ggcataattt
     7321 gagcgccatc cctgtcggca tagcgctacg tacgccaaat ttacgcgcgg gataattggc
     7381 ggtgctgatt accccccgtc gttcgcggct gccgccaata gcaataggga tatcgcgcag
     7441 ggcgggattg tcgcgcatct ccaccgctgc gaaaaagcag tccatatcca catgaatgat
     7501 tttacgcatt gctcacctct caacactggt aaagtataca gtgatttcag ggtttgagaa
     7561 atgcgtaaag attcagcatt taacgcgctc gctgcgaaag cacctgctgc ttatccagct
     7621 tttgcaccag cggctgcacc actttatccc catgctgacc aaagtattga tacagcgtat
     7681 ctgacgcact tttggtcagc accataatct caatgcgccg gttgcccgcg ctttgcggat
     7741 ttttggcatc cagcagcatc tggtccgcca ttgcgcttac ctgcatcact ttattttccg
     7801 gcattccggc ctcttccagc acccgacgag ccgaaagcgc gcgatcgccc gaaaggttcc
     7861 agttgttgta gatattgttt ttgtaggcca tcgcatcggt atgcccggta ataataattt
     7921 tgttatcgag cgagtcgaac actggcgcga gctccaccag cagcgtttta aagaacggca
     7981 taatcttcgc gctgccgcgt tcaaacatat tgcggttctg gtcatcttta atcagcacgc
     8041 gcagtccctg gggaacaatc tccatctcca gattcgcttc catatgtgca tcacgggcaa
     8101 tggtgttgat actggtcgcc agctcgccaa gctcggtggc ggatttcttc ttcagcagcg
     8161 ccgttttctc gttgacgtcg cgcgcttttt tctccgtctc ttccggcacc gccacggtgg
     8221 caggttttgg cggatgagac gggctgattt tatttaacgg cgacaaccca ccaccgttaa
     8281 aaatcgactg cccgtgcagc gcggcgataa tgctttcacg ctcggattta ctgacgctat
     8341 tgacaatcaa ctttatcgtc gtcaccaaag gggccgagcg tatttccgag gtttccgccc
     8401 gctttacctt agacgcgatg cccggtaagc agatggcgat tgacgccgat cttaacgccg
     8461 ggttgatcaa ccaggcgcag gcgcaaaccc ggcgtaaaga tgttgccagc gaggccgatt
     8521 tctacggcgc aatggacggg gcatcgaagt ttgtgcgcgg ggacgccatc gccgggatga
     8581 tgattctggc gatcaacctg atcggcggcg tctgtatcgg gatcttcaaa tacaacctga
     8641 gcgccgacgc cgccttccag caatatgtgc tgatgaccat cggcgacggg ctggtggcgc
     8701 agatcccttc cctgctgctc tccaccgcgg cggcgattat cgtcacccgc gtcagcgaca
     8761 acggcgatat cgcccatgac gtgcgccacc agctgctggc aagcccgtcg gtgctctaca
     8821 ccgccaccgg aattatgttt gtactggcgg tggtgccggg aatgccgcac ctgccgtttt
     8881 tgctgttcag cgccctgctt ggctttaccg gctggcggat gagcaaacgc ccgcaggcgg
     8941 cggaggcgga ggagaaaagt cttgaaacgc taacccgcac catcactgaa accagcgagc
     9001 aacaggtcag ttgggaaacc attccgctga tcgagcctat cagcttaagc ctcggctaca
     9061 agctggtggc gctggtcgat aaagcgcagg ggaacccact cacccagcgg attcgcggcg
     9121 tgcggcaggt gatatccgac ggcaacggcg tgctgctgcc ggagatccgt attcgggaaa
     9181 acttccgcct caagcccagc cagtacgcca tattcatcaa cggcattaag gccgatgaag
     9241 cggatattcc ggcagataaa ctgatggccc tgccctccag cgaaacctac ggcgagattg
     9301 acggcgtact ggggaatgac ccggcatatg ggatgccggt cacctggatt cagcctgcgc
     9361 agaaagcgaa ggcgctgaat atggggtatc aggtgatcga cagcgccagc gtgattgcca
     9421 cccatgtgaa taagattgtg cgcagctata ttcctgattt atttaactat gacgatatta
     9481 cgcagttgca taaccgcctg tcgtcgatgg caccgcgact ggcggaagac ttaagcgcag
     9541 cgctcaatta cagccagttg ctgaaagtgt atcgcgcgct gctgaccgaa ggcgtttccc
     9601 tgcgcgatat tgtcaccatc gccaccgtgc tggtcgccag tagtgcggtg actaaagatc
     9661 atatcctgct ggcggcagat gtgcgcctgg cgctgcggcg cagtatcacc catccgttcg
     9721 ttcgcaagca ggagctgacg gtgtatacgc tgaataatga gctggaaaat ctgctgacca
     9781 acgtggtgaa tcaggcgcaa caggccggca aagtgatgct cgacagcgtg ccggtggacc
     9841 cgaatatgct taaccagttc cagagtacga tgccacaggt gaaggagcag atgaaagcgg
     9901 cggggaaaga cccggtgctg ctggtgccgc cgcaactgcg tccattgctg gcgcgctatg
     9961 caaggttgtt tgcgccgggg ctgcatgtgc tgtcgtataa cgaagtgccg gatgagctgg
    10021 agttgaagat tatgggggcg ttgatgtaag aatccggcat cggtgcacaa ttgccggatg
    10081 cggcgtgaac gccttatccg gcctaccgtt aaggcataga tttgtaggca tgataagacg
    10141 cgctagcgtc gcatcaggca tctgcgcaca accgccggat gcggcgtgaa cgccttatcc
    10201 ggcctaccgt taaggcacag atttgtaggc cggataagcg taagcgcatc cggcaccggc
    10261 gcacaattat aaaataatac gctccccggc actaaaatcc gttacgtccc ccgcccaatc
    10321 gatgggatat aaacctttcg cgacatcgcg atggaacgtt gagaatggcc aatcactcac
    10381 ctgctttacc cagccatgtt tcactggatt tatgtaaata taatcaacat gatgacgata
    10441 atctttggta ttgcgaatgg catgctccca aaaacgcggt tgccacatat tttgcaatcc
    10501 acaagcatgg gtgaactgtt ttttaatctc ccgccagcga gaggaaaaat catcatcgct
    10561 ttcgggtaat gtccagatac agtgcatatg ttccggcaaa acgacccagg cgttaatttc
    10621 aaaaggtctg gcctgcttaa ctttaataat ggcgttacgg agtgtctggt actgggtggt
    10681 caaaagttgg cttcgacgat tccgtaaatt taccgtgaaa aaccatgttc cccccttgat
    10741 gtaataacga cgatattcag acatggcgac tcctttttct caacgataag aagaaaagga
    10801 aaagtcgaca atgaagttgt gttatatcgg gaaagcagct cgcaaaaaat tcgcagactc
    10861 gtaggcatga taagacgcgg cagcgtcgca tcaggcatcg gtgcactatt gccggatgcg
    10921 gcgtgaacgc cttatccgtc ctacaaaatc gtttcctccg tcaaaatcct gcgcgcgcct
    10981 aaaaaatggt cctgccagaa aggttcggct aaccgactta cccgaatggt ttccccggtg
    11041 cgcggtgact cgataaactg accgtctcca agatatacgc ccatatggtc ggctatctca
    11101 cgactgtgga tatggaaaaa cagcaaatct ccccggcgca ggtcgttatt cgccacaatc
    11161 gttgcccgat ggtagtgata catctcattg gccgtacgcg ggagcttcgc ctcgaggatc
    11221 ttgttgtagg cataaaaaac caacccgcta cagtcgaagc cttgatcggg ccgcgtgccg
    11281 ccccagacgt aaggcttacc aagctgctgc tccaggcggt gaatcgccac ttcggtgatg
    11341 ttgtgtagat ggtcgctgct taagaaatgg ttgttctccg ccagcgcctg ccagcgtctg
    11401 tcactcgcct tgagcggcgc aggaaaccat tctggatgct gttttatctg ctcccgatta
    11461 tgctggcgca gcgcccttct gcttttggca ttgccttcca caatatagct ggcctgcttt
    11521 ttcagatggg tttggtactg ttttaataaa cgcgcacggt tttgcatccg ctggcgggcg
    11581 gcgtagctga aagagatatg tgatgtggtg gcgctggcga gtaaagggag caaaacgcat
    11641 aacgaaaaca cgcctggatg cagaaatctg ccgtgcaaga acgaagacat gaaggacata
    11701 aaccagctga atgaaggtga gaattttaaa agaccggagg agtaaacggg attattaatt
    11761 gcttatttgc atattactat tgcggtttca taatcttagg aaataagaaa taaatctaca
    11821 atccatgcgc ttttcggact cttgaaaact gcccctgtgc taaatttgta aaccctgcgc
    11881 cgacaggctt gcgtaaaact cttttggtga taatgtgagc cttccaaatc cagactattc
    11941 cgatttggga ttcattttgc cgtcctggcg tgtgtaaccg ttttatcaag gaataagcag
    12001 tatgcgtaaa atcgcattaa ttcttgcgat gttgttgatc ccgtgcgttt cgtttgccgg
    12061 tttgctgggc agcagtagct cgaccacgcc agtgagcaaa gagtataagc agcagttgat
    12121 gggatcccct gtctacatcc agatcttcaa ggaagaacgt acgctcgatc tctacgtcaa
    12181 aatgggcgag caatatcaac tgctcgacag ctataaaatc tgtaaatatt ccggcggctt
    12241 agggccaaaa cagcgtcagg gcgatttcaa aagcccggaa gggttttata gcgtccagcg
    12301 taatcagtta aaaccagaca gccgttacta caaagcgatt aatattggat tcccaaacgc
    12361 ctatgaccgt gcgcatggtt atgaagggaa atacctgatg attcacggcg attgtgtttc
    12421 catcggctgc tacgcgatga ccaatcaggg tattgatgag atcttccagt tcgttactgg
    12481 tgcgctggtg tttggtcagc cgagcgtgca agtgagtatt tacccgttcc gtatgaccga
    12541 cgccaatatg aagcgccata aatattccaa ctttaaggac ttctgggagc aactgaagcc
    12601 aggctacgac tactttgagc aaacccgtaa gccaccgacc gtttctgtgg tcaatggccg
    12661 ttacgtggtc agcaagccgt taagccacga agttgtgcag ccacaactgg catcaaacta
    12721 cacgctcccc gaggcaaaat aagcgccatt cacctggcat aatcttttgc caggtttcat
    12781 tgcccgtcag cggctgtgtc gcaatcaccg tgaccacatc attcggtgtg gtctgcgagc
    12841 tgaagtcgat ttccacatcc tgatccagca acgttgccac gccaaacggc gcgcggcggg
    12901 tgatccagtg taaattagtc gagcaatacg ccattacata gcgcccgtca gaaagcagca
    12961 tgttgaaaac gcccttctgc cgcagttcat ccgccagtga ggcgatatat ttaaacactg
    13021 ccgccatgtt gcccggtgtg cgcgggtaac gctgcgttaa tttatgcagg agccagcaaa
    13081 aggctttttc gctatcggtt tcaccgaccg ggcggaagtt gccggtttcc agtgatttgt
    13141 agcccgtcag ttgtccatta tgggcataag tccagttacg cccccataac tcgcgggtaa
    13201 acgggtgagt attttccagc gccacctcgc cccgattagc ctggcgaata tgagccacca
    13261 ccgaacagga ttttatcggg tagtcctgga caagtttggc gatgggggaa ttaaagctgg
    13321 gttgcggatc tttaaatgtg cgacagcctt taccttcgta aaaggtaatg ccccagccat
    13381 ctttatgtgg cccggttcca ccaccacgct gtacaagtcc ggtgaaacta aagcagatat
    13441 cggtagggac gttggcgctc atcccgagca attcgcacat gggtaacctc cacaacagcg
    13501 ggagcgcatg gcccccggcg aagattgatt aaaaagtaca aaggttcagg ctgttaccct
    13561 caccccggcc ctctccctga aagggagaaa agaccttact taaccatctc tttttcaatt
    13621 aactggatca ggatatggat cactttaatg tggatctcct gaatgcggtc ggcataacca
    13681 aagtgcggta cgcgaatttc gatatccgct gtgccagcca ttttgccgcc gtctttaccg
    13741 gtcagggtga tcactttcat ccccttctca cgcgccgccg cgatcgcttt gatcacgttt
    13801 gcagagttac cggaggtgga gatccccagc agtacatcgc cttcgcgacc taccgcttca
    13861 acgtaacggg agaaaatatc attgaaacca aaatcgttac cgacgcagga aatatggctg
    13921 acgtcagaaa tggcaatcgc cgggtagccc ggacggtttt cacggtagcg accggtcaac
    13981 tcttcggcaa agtgcatagc gtcgcaatgg gagccgccgt tgccgcagga aagcactttg
    14041 ccaccggctt taaagctgtc tgctaacagg accgccgcgc gctgaatggc gtgaatattg
    14101 gcgtcatctt ttaaaaaatt agccagcgtt tccgccgctt cgttcagttc gttacgaata
    14161 agatcctggt acatgaggat atccttcagc ataaatgtaa tagacaaaat gcagtgtacc
    14221 ggataccgcc aaaagcgaga agtacgggca ggtgctatga ccaggacttt ttgacctgaa
    14281 gtgcggataa aaacagcaac aatgtgagct ttgttgtaat tatattgtaa acgtattgct
    14341 aaatgttttt acatccacta caaccatatc atcacaagtg gtcagacctc ctacaagtaa
    14401 ggggcttttc gttatgatga ttttgagtat tctcgctacg gttgtcctgc tcggcgcgtt
    14461 gttctatcac cgcgtgagct tatttatcag cagtctgatt ttgctcgcct ggacagccgc
    14521 actcggcgtt gctggtctgt ggtcggcgtg ggtactggtg cctctggcca ttatcctcgt
    14581 gccatttaac tttgcgccta tgcgtaagtc gatgatttcc gcgccggtat ttcgcggttt
    14641 ccgtaaggtg atgccgccga tgtcgcgcac tgagaaagaa gcgattgatg cgggcaccac
    14701 ctggtgggaa ggcgatttgt tccagggcaa gccggactgg aaaaagctgc ataactatcc
    14761 gcagccgcgc ctgaccgcag aagagcaagc gtttctcgac ggcccggtag aagaagcctg
    14821 caggatggcg aatgatttcc agattaccca tgagctggcg gatttgccgc cggagctgtg
    14881 ggcgtatctt aaagagcatc gcttcttcgc gatgatcatc aaaaaagagt acggcgggct
    14941 ggagttctcg gcttatgccc agtctcgcgt gctgcaaaaa ctctccggag tgagcgggat
    15001 cctggcgatt accgtcggcg tgccaaactc attaggcccg ggtgagctac tgcaacatta
    15061 cggcactgac gagcagaaag atcactatct gccgcgtctg gcgcgtggtc aggagatccc
    15121 ttgctttgca ctgaccagcc ccgaagcggg ttccgatgcg ggcgcgattc cggataccgg
    15181 gattgtttgc atgggcgagt ggcagggcca gcaggtgctg gggatgcgac tgacctggaa
    15241 caaacgctac attacgctcg cgccgattgc gaccgtgctt ggtctggcgt ttaaactctc
    15301 cgacccggaa aaactgctgg gtggcgcaga agatttaggt attacctgtg cgctgatccc
    15361 gaccaccacg ccgggcgtgg aaattggtcg tcgccacttc ccgctgaacg tgccgttcca
    15421 gaacggaccg acgcgcggta aagatgtctt cgtaccgatc gattacatta tcggcggacc
    15481 caaaatggcc gggcaaggct ggcggatgct ggtggagtgc ctctcggtag gccgcggtat
    15541 caccctgcct tccaactcaa ccggcggcgt gaaatcggta gcgctggcaa cgggtgcgta
    15601 tgcgcacatc cgccgtcagt tcaaaatctc tattggtaag atggaaggga ttgaagagcc
    15661 gctggcgcgt attgccggaa acgcttatgt aatggatgct gcggcatcgc tgattaccta
    15721 cggcattatg ctcggtgaaa aacctgccgt gctgtcggct atcgttaagt atcactgtac
    15781 ccaccgtggg cagcagtcga ttattgatgc gatggatatc actggtggca aaggcattat
    15841 gctcgggcaa agtaacttcc tggcccgtgc ttaccagggc gcaccgattg ccatcaccgt
    15901 tgaaggggct aacattctga cccgcagcat gatgatcttc ggacaaggag cgattcgttg
    15961 ccatccgtac gtgctggaag agatggaagc ggcgaagaac aatgacgtaa atgcgttcga
    16021 taaactgctg ttcaaacata tcggtcacgt cggtagcaac aaagttcgca gcttctggct
    16081 gggcctgacg cgcggtttaa ccagcagcac gccaaccggc gatgccacta aacgctacta
    16141 tcagcacctg aaccgcctga gcgccaacct cgccctgctt tctgatgtct cgatggcggt
    16201 gctgggcggc agcctgaaac gtcgtgagcg tatctcggca cgtctggggg atattttaag
    16261 tcagctttac ctcgcctctg ccgttttgaa gcgttatgac gacgaaggcc gcaacgaagc
    16321 cgacctgccg ctggtgcact ggggcgtaca ggatgcgctg tatcaggcag aacaggcgat
    16381 ggacgattta ctgcaaaact tcccgaaccg cgtggttgcc gggctgctga atgtggtgat
    16441 cttcccgacc ggacgtcatt atctggcacc ttctgacaag ctggatcata aggtggcgaa
    16501 gattttacaa gtgccgaacg ccacccgttc ccgcattggt cgcggtcagt acctgacgcc
    16561 gagcgagcat aacccggttg gtttactgga agaggcgctg gtggatgtga ttgccgccga
    16621 cccgattcat cagcggatct gtaaagagct gggtaaaaac ctgccgttta cccgtctgga
    16681 tgaactggcg cacaacgcgc tggcgaaggg gctgattgat aaagatgaag ccgctattct
    16741 ggtgaaagct gaagaaagcc gtctgcgcag tattaacgtt gatgactttg atccggaaga
    16801 gctggcgacg aagccggtaa agttgccgga gaaagtgcgg aaagttgaag ccgcgtaatt
    16861 gaataacgga gccgaaaggc tccgtttctt tatccgctaa ttatttaaaa ttaaagccat
    16921 ccggatggtt ttccaggctg ccggtcaacg ccgcgaacag caccgtttta ccatcaatag
    16981 aaagcgcatc gttcacattc aaccaggtga gtttctcttg cgacgttttc tcatcaatag
    17041 tcgagaacag ccccgtcatc tgattagatt tctcggacca catcacagcg atacgttgcg
    17101 agccacagtc atgcggtttg cacgcgctca tcacctgata cgtctcatct cccaatgtca
    17161 cggtttgtgc gggagtataa gtaccgcctt tcatcaccca ggcaggcagc ttatgccctt
    17221 gcaccatctg attaaacgcc gctttggtgg tttcgccctt tgcaagactg ctaatggtta
    17281 aatcatcctg cgccattgca ctggtggcga tgaccagagc ggcgactgtc gttattgcct
    17341 taaacatcat tcctcccggg cttatcctgc ccatgtgatt aactccctaa agcctggcga
    17401 taacacgata atttgcaaat cagatatttc caatatcagc atttgttatc aatttattct
    17461 gcgctaaggc aactgcctga taaggtgtta gagttataaa acccttcgca taaaggagtt
    17521 gtacaccgtg cctggtttga agattacgct tttgcagcaa ccactggtgt ggatggatgg
    17581 tcctgccaac ctgcgccatt ttgatcgtca actggaaggt attaccgggc gcgatgtgat
    17641 cgttctaccg gagatgttta ccagcggctt tgccatggaa gcggcagctt cgtcgctagc
    17701 acaagatgac gtagtgaact ggatgaccgc taaagcgcag cagtgcaatg cgctgattgc
    17761 aggcagtgtt gcattacaaa cggagtctgg ttcggttaac cgctttttgc tggttgagcc
    17821 gggcggcacg gtacattttt atgataagcg tcatctgttc cgcatggcag atgagcatct
    17881 gcattataaa gcgggcaatg agcgagtgat tgtggaatgg cgcggctggc gtattttgcc
    17941 gctggtgtgc tacgacttac gttttccggt gtggtcgcgc aatctcaacg attatgacct
    18001 cgccctgtac gtcgccaact ggcctgctcc gcgctctctg cactggcagg cattgctgac
    18061 agcccgcgcg attgagaatc aagcgtatgt ggcgggatgc aatcgcgtcg gcagcgatgg
    18121 caacggctgc cattatcgcg gtgacagccg ggtgattaat ccgcaaggcg agattatcgc
    18181 tactgccgac gcgcatcagg caacgcgcat tgatgcggag ctgtcgatga tggcattgcg
    18241 ggaatataga gaaaagtttc cggcatggcg ggatgcggat gagtttaggt tgtggtgagt
    18301 gggggttgtg tttagggctg gggagagtca gggtaagact acgaaagccc gctcccccag
    18361 ggaaagatta cgaaagcccg ctcccagcaa ggactgacgc caggtagttt ctgtccatgg
    18421 ctgcttttcg catcttacgt cttaaccctg ccttgaatac attatcattc gttaaaatat
    18481 taatagcg
//
LOCUS       DAZGAP010000106        18030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGAP010000106 DAZGAP010000000
VERSION     DAZGAP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.106"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(295..384)
                     /locus_tag="ACHLAM_003155"
                     /pseudo
     CDS             complement(295..384)
                     /locus_tag="ACHLAM_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(527..1102)
                     /locus_tag="ACHLAM_003156"
     CDS             complement(527..1102)
                     /locus_tag="ACHLAM_003156"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO7854641.1"
                     /translation="MLSADQIAQLSRTRSLLNHTSEWIPLSGQQITRLTELPLAYNLQ
                     RSAQLLQQLRVLFPDNPRVQEMVDNWQKSVRSRALPEEAMTGWNEGMTRLQQLAERLN
                     RLDEQRGKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYNEVRNQNGSEQQQKLTQDA
                     LTELLNRYWFLRHGDAGKKTINNKQDNQINI"
     gene            complement(1136..1285)
                     /locus_tag="ACHLAM_003157"
                     /pseudo
     CDS             complement(1136..1285)
                     /locus_tag="ACHLAM_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1610..1924)
                     /locus_tag="ACHLAM_003158"
                     /pseudo
     CDS             complement(1610..1924)
                     /locus_tag="ACHLAM_003158"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(1979..2284)
                     /locus_tag="ACHLAM_003159"
     CDS             complement(1979..2284)
                     /locus_tag="ACHLAM_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854642.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            complement(2350..4095)
                     /locus_tag="ACHLAM_003160"
     CDS             complement(2350..4095)
                     /locus_tag="ACHLAM_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7854643.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLMYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            complement(4330..5532)
                     /locus_tag="ACHLAM_003161"
     CDS             complement(4330..5532)
                     /locus_tag="ACHLAM_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO7854644.1"
                     /translation="MILVCVILWGIYVAVRALINLNERFIDAVSSPAGIIGLFFGLLT
                     VFAILLRFFIYRRLRKKTAAFEQAVSELVQRERGFNETVNAVIARGIRQEKEQLARRR
                     EEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVSEK
                     IAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQIREEKERQDELDRRQR
                     EAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMAQL
                     TKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDDAP
                     ALEKTLHDSLEKYRINRINLRKEFFRVKLEKIINEVERHHGQMEYVADPAALQYLQSL
                     EYAENEAA"
     gene            complement(5729..6031)
                     /locus_tag="ACHLAM_003162"
     CDS             complement(5729..6031)
                     /locus_tag="ACHLAM_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854645.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(6378..7382)
                     /locus_tag="ACHLAM_003163"
     CDS             complement(6378..7382)
                     /locus_tag="ACHLAM_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO7854646.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKFGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(7640..8071)
                     /gene="tssE"
                     /locus_tag="ACHLAM_003164"
     CDS             complement(7640..8071)
                     /gene="tssE"
                     /locus_tag="ACHLAM_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7854647.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(8074..8610)
                     /gene="tssJ"
                     /locus_tag="ACHLAM_003165"
     CDS             complement(8074..8610)
                     /gene="tssJ"
                     /locus_tag="ACHLAM_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7854648.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(8591..9691)
                     /gene="tssG"
                     /locus_tag="ACHLAM_003166"
     CDS             complement(8591..9691)
                     /gene="tssG"
                     /locus_tag="ACHLAM_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7854649.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(9646..11409)
                     /gene="tssF"
                     /locus_tag="ACHLAM_003167"
     CDS             complement(9646..11409)
                     /gene="tssF"
                     /locus_tag="ACHLAM_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7854650.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPISVCNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(11543..13141)
                     /gene="tssA"
                     /locus_tag="ACHLAM_003168"
     CDS             complement(11543..13141)
                     /gene="tssA"
                     /locus_tag="ACHLAM_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7854651.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKNIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(13141..16530)
                     /locus_tag="ACHLAM_003169"
     CDS             complement(13141..16530)
                     /locus_tag="ACHLAM_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO7854652.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPARFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(16523..17671)
                     /locus_tag="ACHLAM_003170"
     CDS             complement(16523..17671)
                     /locus_tag="ACHLAM_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO7854653.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREIMDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCFGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(17675..17941)
                     /locus_tag="ACHLAM_003171"
     CDS             complement(17675..17941)
                     /locus_tag="ACHLAM_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7854654.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
BASE COUNT         4475 a         5038 c         4164 g         4353 t
ORIGIN      
        1 gcagactctg actaaccaat atatacaatc tccctccaca ctctcatgca gcagccagct
       61 aacagtagtt gactgcctca acttcagggt gacttctgcc gttacttttt taacccctcc
      121 cccagcgcct aaacgacact ttcgttttct gcagtgcctc ataggaagta tgagcttcaa
      181 aactggtgta atcaaaaccc gctgacgcta ccattctgac aaatatgcct gtgaatgata
      241 cccgactttt ccatcatgat gcctgtttag tctggcgcgc accgtacgga tgacatcttt
      301 cacccggcca gtttcataca acgcatcgta agctttgtac gcatcagcct gcaactctca
      361 cccatattct gccagatgac gttgtgaatc ttcgagggaa agccgcttta tcatctgcgt
      421 attctgttct atttgatcac agattggtcc aatctgtgat caattcagat gtcattttgc
      481 attaatggca tcatcgcttc tggataaata atggcaataa aatctgtcag atattaatct
      541 ggttatcctg tttattattt atcgtcttct tacctgcatc tccatgtctc agaaaccagt
      601 agcggttcaa cagttccgtc agggcatcct gcgtcagttt ttgctgttgt tcactgccat
      661 tctggttgcg gacttcatta tagcgacgta actgctcttc tgccgggata tgccggttaa
      721 aagcctgcat gatgccaaac acctctgttt tcagttcact gactgtcatg tattttcccc
      781 gctgttcatc cagacggttc aagcgctcag ccagctgctg taggcgggtc atgccttcgt
      841 tccagcccgt catcgcctct tccgggagcg cacggctcct tacactcttc tgccagttat
      901 ccaccatttc ctgtacccgg ggattatcgg ggaaaagtac cctcagttgc tgcagcagct
      961 gcgcactgcg ctgcaggttg tatgccaggg gtaattctgt cagtcgcgtt atctgttgac
     1021 cggaaagggg tatccactct gacgtatgat ttaacagtga gcgtgttctg ctgagctgtg
     1081 ctatctggtc tgccgaaagc acatccggtg ggggtgtcag cagcgccctg agttgctgct
     1141 ccgccggtga tggctgtgtc agaaactgcc atcccggaat ggccacgctg acagccagca
     1201 atgccgcaca gacaccagcc aaaaacggct tcagaaacat gggcggggat ttctgaaggg
     1261 agggtgtggg ctctgctttt gtttcagaaa caacatacac cagacgggag tgcggcaggg
     1321 tctgatgttc ctgcacatcc ggagcatcag accggagtgc ctgagccgga agcgttaccg
     1381 gagacgacgc ttcgccctgc acaggctgat tttccaggcg ggtgatatat cccttcacca
     1441 tggcttccgg taaagccact ttactgccct gtttaagttc gtgccgggca agtgtgtcac
     1501 tcagtgcctt caggaatgtt tcggtctgat acagcagggg caggttgtca cggtcgtccg
     1561 gaggcatggc ccgaagcgtt ttttgcagac ggttgaacag accggtaata atttccagcc
     1621 gggcgtgggt attcagcggc cacatcactg accagtgatg ccgggtcagc gccacaatca
     1681 gagccagccc ttcattcagt ccacttaacc ctgctgtatg catccgggcg atggtgtacc
     1741 aggatgccgt ctgtaattcc acgccattgt gttcatacag acgcaggcag agcgtttcca
     1801 cgtaacgcca gtccacatcc ggacgtgccg ggtgggtcag tttgctgatt tcatcacgca
     1861 gggcagcata atccggtaat gacctggggt caccaccggt tttcagtttc acttcagaag
     1921 tcatcggcag attcggtgtt actgtattaa agcatgatta acagtgacga aaagaatgtc
     1981 agtgtaatac cgggacccgg gaaaggtatt ccacggcacg gatgattgcc ggtgtcacat
     2041 cccaccgccg tttcagaacc ggtaacagct cattaaggtt tctgatataa cgttcagtga
     2101 acagccgttg tccgtccctg gttaaatgaa aaccggactg attacaggta atggtgatat
     2161 gacaggtatc gctgccaata tagacagctt cctggggtct gaatctgaca ttccagtgcc
     2221 aggagtacgg agagtggttc agataaaaaa gacgcatctg aatatcatta aataacgggt
     2281 tcatggcgaa ttaaataaac ctgataataa cagtatgatg aaagtccgga ataaagaaat
     2341 aatccggtat tatgcaaaat cctcaaggtg acgactgata ctgttttcac ggtaacaggt
     2401 caccagtaat tcacgggttg cccgggtgaa cgccacataa agcaccggaa tcacttcaga
     2461 ctcatccgct gcaccagagt gaacgaaaga cgcattgata accgccacat acgggaactc
     2521 cagccccttg ctgctctggt acatcagtaa atgcaccaca tccttacgtc gcgagtatgt
     2581 cttcttgcct tccctgtcaa aacaggtggc aaaaggcacc ccgcgtagtg tcataacgtc
     2641 ctgcaattgt tgcgctgaac gctctgtcgg acacagcaca gccatatcac tccagtgacc
     2701 acacagggca tggcgcttct ccagccagtc tgcaacttgt ctggcttcat cacgttcaga
     2761 agcacaacgc aggatttccg gctccgtacc ctcttcaccg cccgcctgtg gctgaacgag
     2821 cggtatttca cggttctgat gtttttcaaa atactcgcgc gagaatgcat aagcaaagtg
     2881 cagaatgcgt ttcggattac gataattgac cggcaggaca gaagtgcgtc cctgtgcctg
     2941 aatccccaca ctggccagag aaaagttcag ggcccgctcc cgccggtaaa tggactgggc
     3001 gtcatcgtac atcaggagca gtgaacggga tgcgttatca aacagacggg caatcagggc
     3061 cagccagcga ctgtcaaaat catgcccctc atccaccagt acagcatcat atccggttcg
     3121 ggtgattttt ccgctgttca cggcctcttc cagtgcactg aagcatttat caggatattc
     3181 ttttctgtcg gccgtcacct taataccgtg gcgttttacc attgaggcac accagctgtg
     3241 aaaatgggat accgtaaccc gtgacgccaa ccctctggct tcaatgcatt cccgcagata
     3301 gctggccagg gtaatgttgt agcacaacac cagaatcttt ccgggtgtgg tttcagccag
     3361 gtaaaggcaa cggaacaaaa gaatcatggt ttttccggaa ccggccacac catgaatgac
     3421 acgatgcccg tcaccaatat ttctggccag aatctcctgc tgaatatcca tgattttgat
     3481 ttgtgagttc tgtttcacgg tcacttcagg aaacagatgg gcgcgcagaa tatctctcat
     3541 ccggggtgtc acccgggttc tgaatcccgt ggtaaacatc ccggcaattt tttgctggaa
     3601 tacctcaggt aaaacagact gcgtcatttc atcctggcag attgtctgtg ctgaaggaaa
     3661 aattttttcc accgcattct catcattccc tgccagtgtc ttcagttgct gacgggtaat
     3721 gcgggtaaac accacaccat acgcccaggc caggttcagc cgtcccttat actgaccgtc
     3781 attctgccgt aagcacgggt ttgcaggtaa ggcattaacc gtatcacagg cataacgacg
     3841 aacctgtacc agcggattga tttctgattt cagaagaccg tcggtttcca gcgtgacctg
     3901 ctcctgattt acctggcgca gcgtggaaac cgtccagtcc ttgacctcca gaaataccag
     3961 tccgttctcc gggtcaataa tgacaaaatc cggatgccgg tttttccgtc ccaccggaat
     4021 atcgtaccag acaaggcagt catcattcag aaagctctcc agtcgtgaag ccaccctcct
     4081 ctccccaggc gtcattctct cctcagtgga ggccgataca tttatcagtt tcgccataaa
     4141 cgcaccttaa tctgtgaaca aaaaataatc accaccgaca tccacggatg ttatatcgtg
     4201 ggtgtcgctt tattcagaaa taaaaaacag aatcattaac atgaccagaa tactgtgaac
     4261 catgctgcag ggtatctgca gatatccctt ctgttatatc cggccggggt aaaatcagaa
     4321 cactgactgt caggctgctt cattttctgc gtattccaga ctctgcaggt actgcagcgc
     4381 cgccgggtca gccacatatt ccatctgccc gtggtgacgc tccacttcat taatgatttt
     4441 ctcaagcttc acgcggaaaa attctttacg caggttaatc ctgttaatgc ggtatttttc
     4501 cagactgtca tgaagtgttt tttccagcgc aggcgcatca tcacaggaaa tcatggcgtg
     4561 aacatcaaaa tcaaacggaa cagaagcacc acttaactct ttgaccctgt ccatgggttc
     4621 cagccgtcgg gtcatgccaa ttttaaagac atcctcacca aacgaaccaa tattcgaaat
     4681 gatataaaca tgcccctgtt tcgtaagctg agccatggat ttagcacgtt catactgcgc
     4741 atgtgcttcc tgaattttct gttccagctc caggcgttgt ttctctaact cctccctgtg
     4801 agccccttct gctgcgcgca gcgcctcctc aatcagtttc tgctgctcag caagacgccg
     4861 ttcttcctct tccgcttccc gctggcgccg gtcaagttca tcctgacgct ctttttcttc
     4921 acgtatctga cgcttaagct ctgcctgata ttccttctct tcctgcgctt tttccagcaa
     4981 tgccactttg tccagaaact gttgcctgac actctcccag tcagaattat cttccaggtt
     5041 aagaaaggct attttctcac tgacagcagc gtaagcctct ctttccttac gaagctggtc
     5101 atatttgctt tgccagttat taatcgttac accagccagc agagtcttcg ccttaaattt
     5161 ccaggcggaa tcaacaatcc gttgcattgc acggctggcc ttctgtctgg cggtatgaaa
     5221 ttcctcacga cgtctggcca gttgctcctt ttcctgtcgt attccccggg ctatcacggc
     5281 attgacagtt tcattaaaac cccgctctct ctgaaccagc tctgaaacgg cctgctcaaa
     5341 ggctgctgtt ttctttctga gtctccggta aataaaaaaa cgaagtaaaa ttgcaaatac
     5401 agtaagcaaa ccaaaaaaca aaccaataat acctgccgga ctcgatactg catcaataaa
     5461 gcgctcatta agatttatca gcgccctgac cgccacataa atcccccaga gaataacgca
     5521 aactaaaatc aggacaaatc ccgctgcatt atcatctttt ttcttcctgc catgactttc
     5581 ctttattttc cttctcagta actgaatatc tttgattaac gccctccgga aatatttata
     5641 cccggaaggc tgtccagctt tatgataccc gccagtacag aattaactgc aggctgagca
     5701 cagatttctc tgctatttca gtgtcagatt aatactgatt accggaacaa atgggcagat
     5761 gtgtaaatcc aggtttgctg gccatataaa gcggcgcaca ggtgggttta cggggttcag
     5821 tgaccggtac aacctcattt ttcccggcca ccgacggttg tgacaccttc cggacagccg
     5881 ctgatttttt cttctgaacc agcttcgtgg tgaccggtga tttcacctga cgtttggcgg
     5941 gtgccacgcc ataaccggaa caaaagcgct tcgactgact cagacttccg tcattgcaga
     6001 caaaacgtcc gtctgacgtg cagtgtgaaa taccaccttt agcacccgag cagggttttc
     6061 ttccccgcgc agcatctgtc tgaaccgaaa ataatctatg gtcgcccccg tttttgcaac
     6121 actgattttt taattaatgg tgggcttgct taaatctatc cggcgtctct gtaggcaagg
     6181 atgcctgcgc cccgatgagt ttcgcacctg ataatcctaa aaagcccctc ggcttcagtg
     6241 agccatttta tttattaggt ttttaatcag gtcgatttgc cgttcatgtc atcaataatc
     6301 agtcttcgca aaaccagaca ggcaactttg tatcttattg tttacaataa gccaattgag
     6361 ttatatcagc atttatatca agcctgatat tctttttgct gagtagtgat tacccaggca
     6421 attcgagcca gtttatttgc cagagcgcag gcaaccacat tcgaatgctt cttgttgagt
     6481 tgctcccgaa cccattcggc aagttttcca tgctggcgtt caagccgcat catgaatgag
     6541 cgggcacact gaaccagtaa ccggcgcaaa tttttatcgc ctcgcttact gatacccaac
     6601 agagtcgatt ttcctcccgt gctatactgc cggggaacga gccctgtgga agccgcaaaa
     6661 tctctgctgc atgaaaactg tttcccgtca ccaggttgcg acgatagtaa gctggcagta
     6721 atcggcccaa caccaggaat tgtcataatg cgctgagcgg tatcatcagc gttgattgac
     6781 tgacttaatt ctgattccag ctctgctatt tgctcaacaa ggtaaaggta gtgggtatgc
     6841 aacctcacca aaaggcggct cagatattcg gggatttcgt gttcagccaa cacgagagac
     6901 aaccgtttta ttacagcatc acctgtcggc aggctgattc caaattccag caagaaaccg
     6961 tggatctggt tggttgtttt caccttatct ctgatgagtg attctcggac ccggtgtaac
     7021 gccctcatgg cctgcagggg ctcagttctt ggctggacaa aacgcattga tggtcgtgaa
     7081 gccgcttcgc atatggcttc tgcatcaaca aaatcattct tattactttt gacaaacggg
     7141 cgaacgaact gtggagaaat tagcttagtt tcatgcccaa aatcagagat acgacgagcc
     7201 ataaagtgtg cacccgcaca ggcttccatt acaacaactg agggcggaca tgtcgccaga
     7261 aactgcatca gtttagtacg ggtaaatttt ttacgcagaa gagccttacc aaacttgtct
     7321 tgacaatgaa tgtggaagga gtgctttccg agatcgatac caataagtgt gatgttctgc
     7381 atgatggtct ctccgttaaa gggattcacc ttaacagcgt actgctgtta aggtgggggt
     7441 gaccatctca ttagtcccac gaacagaaaa cagataattt tcttcattta aaaactcata
     7501 cattgttatt gctgatatgc cagagttatc aggatgatga aacattccgc cagcgttgtc
     7561 ataagccgga ataatgtcac attcacacct gacatatatc catcactgtt tatttctgtt
     7621 ctgccctccc tgctccggct cacgtctgaa cgtaccgctg ctgtttcaga tggcgcaaca
     7681 acaccctgcc ctccggtata aatgttgttc cgtaacgaac cagcccggca tctttaagtt
     7741 ctgcatcaat gacataatgc aactcaccag gctgagtctg ctcctgcatc gtcacgttga
     7801 cacgcttaat acgtggctca tacttcagca gaacatctga aagcacacgc atcagttgat
     7861 gcgccgttcc cggcattccc tgcagaacgg ttgtcatatc cggcagacca taatccggca
     7921 gatgtttaag gcttccggcc cgggtattca gaatacgctg catgttatcg agcaccgaaa
     7981 gaataacctg ctcttccgca ctgacctggt ttaactccag cccgccagcg aaattgccat
     8041 agagaatttc ataaagggaa ggacgcggca tcactattta tccttcaccg gtaacagcgt
     8101 cagggtatta ccggatactt caataagacg gggcgtgtcc ggctcaagct catcccggct
     8161 cagcaccacc cgccaggtat ttttcttccg gtcaggctcc agaaacattg ctgccacacc
     8221 ggtaaatttc gccgcatcat ccagaggcat atccacggtt actgagccac ctggctgcat
     8281 ccatacatct ttctgtgcaa tgatatcgcc tgccagaatt tcactgtccc ccgtaaaaag
     8341 cgcctgataa tccgcgctgt caaaattccg gttgtcttta agctgataaa tccgcaccac
     8401 aaccgacagg ggaataccgg cagcactggt gttaatggcc tccctggcac gaatatcaag
     8461 gtgaagtgtt tttatctgtc ggtaaaacag ggatttcgtg gcagataccg taccgtctgc
     8521 cactctttgc gtcaggccac accctgataa cgacagggaa aacaacgcaa taatcagacc
     8581 gtaaccagcc ttaccagcga taatcgccat tttcctgagc ctccttacgt tgataatgtt
     8641 cctgaacccg ctgataacgc cccagacgga tggtcattac gcgtgccgct gaagtcgtta
     8701 tctttgcctg tgttctcatt acagccgtgc gccccagttg cggactaccg gctgccggtc
     8761 ggcaactgag ccggacatcc ggcaacagtg aacgctcaac acaaagttgc agacgtacat
     8821 ccagacggct tcccagataa acgtgcaaca acgccagcaa atcagaatac agctgcccgc
     8881 ccggtaacca gccctgtacc tcgctgcccg tctgtgttga aagctgtaat aacacctgtc
     8941 cgttcacatc cgtagcatga tcgcccatca ccgggcgact cttcagactg accggatgat
     9001 gaacacgcat ggccagcggt gttttcagcg ggatccgtcg tcggtcatga tgccagacac
     9061 gggcctgtgt tccgggagcc agcagcgtta ccagtgatgt cagcccttca gctgttcttc
     9121 cgggtaacaa catcaggggc aataacgcca gaaaacgcga taccggcgtg gcaatattct
     9181 gtgcacatcc ggggatcccc agtctggcca gaccgagcag atactgggat gttttatcct
     9241 gcccccccgc ctcaaaggtg gccgggtacg aatatttgcg ccagatacgg taatactggg
     9301 tgatcagacg atgactgaat atatcgagaa aatccgctgc ggcttcatgg ccttcccgct
     9361 gctgtgcaat atcactgata tagtgagccg gcaacggaga cgtgaccccg taaagtccca
     9421 taaacgtcac atgtacagtg ggtggcagat gggattcttc aggaattaca gcatctttta
     9481 tttcactcgc aggaaacccc attcccgtgt atggacaaaa gcgaaccgct tcctgacggg
     9541 cctgccagcc actgccaatg ggaggctgat ctggctggct ttgttccagt aactgacaga
     9601 agcgataaaa attaatatac ggaagctgtt tccggtaacg ggtaattaac cgggcaggcg
     9661 cggattgtga ttttctttcc accggatgca ttttccttca ggctgaacaa tcagtgtgag
     9721 ctgattgaac tgattcatat cggcgtacag ggccaggaaa cggttgagca tttcgccaaa
     9781 caggtgaata tctccctctc cggcaaaccc gttgccatca agagttacct ccacatcaag
     9841 cccccggagc agaaaaccct tttcaaaacg ctgaatacga tgatgctgta cagccagaat
     9901 ggcatcaagc cgacggtgat taagttcatc gtcccgccag ttataaagcg ccagcgtgcc
     9961 acgcagcact tccgcactgc tcagcatgtt aagaaatcct gtccccagat gactcatcac
    10021 ccgccagtga aaacgatctt ctgtcggcgg ataaaccggc agcgtgggtt tacaaagatt
    10081 acatacactg atcggggtct gcagtacctg ttcacaccga tcaagcagcg tactctgcaa
    10141 cgcacgacgg ggaagctgac cattggtacc tgtaatacgt aatgaaagcg tttcccgctc
    10201 cggcatccga tccgcctccc accgctgacc gccaaggata agccatgtgt catacatccc
    10261 cgtgacaccc cgcttcaccc gggtatgata ataacgttcg ggtgcatcat ggcgcagcat
    10321 tcccccacga tgacggaagc tggtaaaagg cacgtactct gcactgccgg ttcgcccgga
    10381 gcccgccacc gcatccactg aatatatctc cgtatatcca tcctgcagac gcttcgggcg
    10441 cagcagatat tcgctctcca gcccattgat gatgagtgga tcggcttcaa gcgtaaaaag
    10501 gttaatcacc ggcacacaat gcagacacag ggcatcatct gtcacaggta aatctgccgg
    10561 ccaggactga ctcagcacca cctcaatatc aaatccggaa atacctgccg gcaaagaaat
    10621 attctccagt ccgttcagat gaacaaacat aaatttttcc cgaaaggtga aatattccag
    10681 caataactga tacccactga aagcactgtc acctttcggc cacagccgat cttcttccgc
    10741 aaaaccaccg ggtgaaaacc atccgtcaat gcggatccgc tcatcatttc ccggcagcct
    10801 cagatacagc gcagccagac gctttgtcat catcagatga agggtgctgc tgaccggggc
    10861 ttctgctgcc agatacaggc tcagacgatg caaatcgacc tgagaccagt cagccagttc
    10921 actgcaggca aaacctattt tcagcactga gcgcccgtcc ggctccgtag tcataacgac
    10981 cttctccact gccagtggat tcagtggtat ggcccgggtg gtacggtaac gacagactgt
    11041 atttccgggg ccaacagggc gggaagtcac ttccagtccg gctggtacgg tttcagccat
    11101 tttcatgacg gataaccggg gagtcagtgc caccaccgac agggacggga tagtacgcag
    11161 gtaatgaggc cagagcatac tgaccagact ttcagtaagc tccggcaaat catcatcgat
    11221 tttctggcgc agccgtccca tggaaaaggc gaaaccttca aacaggcgtt ccacgcaggg
    11281 atcaggcgta cccgctttat cgagatccag catcgccgcc cgatcgggat gagcctgtgc
    11341 aaacgccttt ccggcttcac gcagataacg catttcagcg tcaaaataac gcagggttaa
    11401 atcgtccatt atcttctcag aataaagcta actgttccgg agactcgcgc tctgacttca
    11461 gccatatttc aaacaatcgc ccgaactgaa ctgcaggcag gatttcatta atcagattac
    11521 acatgaatat aattcagaca tctcatgaac ttcccgaatt gcacaacaca gcagctctgg
    11581 cagcatcaat ggcgatgagc ccggcaagaa gatgttccat atcaggctgc agacgcaccc
    11641 tgtcactttc agtttttgcg gacttcatac gtaacagttt caggcggcgg gcttttacct
    11701 caaataccag ttcaggttcc cattgtgaca gggtcagccg ggttgcacgt tcatccagct
    11761 ccacaagcag atgcagggca aggtcatttt ttccagtctg ttcggcaaca cgggccatca
    11821 acaggcgcag tagccagttg ctgcggtcag actgaatacc gggacgtgcc tgtaaccagc
    11881 ttaaagcggc ttctgttcct tcattatccg caatctgcag ggcttccggc tcaagagata
    11941 aaatgtcgtt atctccggga gctgccgtta tcgttacaga aggttcttcc gcccagcgtt
    12001 caccacactc catcacctgc tgctgtatcc agctcagcgt cacgtcatcg gcaaacggca
    12061 tgccgtcgtt aaaagccagt gtctcaagcc ccggcaggcg cagaagcaat cctttcaggt
    12121 catactgaat aatggcagca taattttctt ttccggtctg caacagcgcc tgatgaatat
    12181 accactgcag gtccagccac agatgactgg ctccccgggc aaacatatcg tcacactgtt
    12241 caagcagtga caaccagttc tgctgcagat acaaccgttt cagtgacgca cgccggtccg
    12301 gccccggtgg ggcaatacgg gtccgtccat cagcagataa cggcggtaac tgatgcagcg
    12361 tgtcatgccg gacgcttttc atcagtcgat gagccgccag ccagccctca ggctggtcac
    12421 gcagatattt tgccagaaca cgggcctgtg acagcagttc ctgtccggag gtgatggcag
    12481 ataatgccat gacgctgccg gatgttaccg tcggtgccgg cgcggcagcc tcctgaggca
    12541 ccactgcatc aacaccaccg gccttcatca ggcgattttc aagtgcctga tataacccgt
    12601 tcagcaccgg ggcggaagcc tcatccgtaa aggtctgttc tgccagccac agcgccccgg
    12661 caatgcgcag ggtatccgcc ttcaccacct ccggatacag agacagactg tccagtatgc
    12721 gggcgctgca taaccactcc agcgccgcct tcctggcccg tgaccgttgt ggatgcaggt
    12781 gctctccaaa acgctgcaac aaaccggtca gcagctccag accgtcggcc agcccttttt
    12841 caccatcctg ccgcagacgg gcccagatat accaggtcgc cacccggata tttttggttc
    12901 tggtggtcag taatttttct gcaagctggc tgacaattcc ggcatcggct ccggagagtt
    12961 tattgacctc ttcacgcatc tgctgaaaat catcatcgta ccccgcatca tcacctgcgg
    13021 gattatctgg cgtcacggga gcaagccatt tatcccacag cgcaatacgc acctgagccg
    13081 gttcagccac atcccggggt gtcgtctggc atgcactgag gagcgaatga atggaagcca
    13141 tcagtcagtc tcctccacgg aatccccggc atcttcgtca ttgcctgtaa acgctgacgt
    13201 accggagagt tcgaaaaccg tttccggcag aacaaaattg cggagtttca gcaatacaag
    13261 cggcccttcc cccgcttcag ttcgcagtgt gtaattcagc atacgaccgt cctgcgcctg
    13321 ccagctcagg ctccagccac tggcaacgcc cggtgcggct ttccgtcggg ccatctcaag
    13381 cagacgtatc agcccccagc ttcccggcag gtctgcatac tgacgggtac cggcctgagt
    13441 gcttacccag cttaaactgg cgccaggtgc ttccgtatca gccggccagg taaatcgctt
    13501 ccataccggc atctgattaa cataaatcag tttctgatta tcggttatca gcgtcgtctg
    13561 catcacaccg gcagcagttc ccgggcgcag ctcaaaatgc agccccgcgt tacctgtggt
    13621 gaacgccaca tccgctattt cactcagggt attgatggct ttcagaaacg cagggttgaa
    13681 tgtcagtccc cgggtattaa tggtatcagg tacccagcga ctgccttcac tgtgcaacac
    13741 accactcagg ttattctgca ggaaacgggc aatacgtccc gtgtccgtat tcaggtattt
    13801 tgccagcagg ggaagcgaag catcgctgct gacattttta aacggataac gcccggaaaa
    13861 tgcattattc cagccatcca ccaccgcagt acgccaccgg gcattcagac tttctgccgc
    13921 gggcgtaaga acctgctgcc acgcctgctc catcgggcga acaaagaccg tctgtccgaa
    13981 gccataccac tcctgtccga gtccggcagc ggtcaggcta ccgtaatccc gggtatcggt
    14041 taaatcaacc gatttcccct gcaatactgt ctgtgccagc atctgcatca tggcctgcgg
    14101 gtcagaagag ccggcaatct gttgcagacg cagacgaacc tgtgtcaccc gggtcagata
    14161 tgtctgcaga ttcagcatat ccgcactgtt gttctgatta tccatcagtg caagcaccgg
    14221 cccgaaagtc gtcgccagag gaccgtgaag ccggctttcc ggtactgcca ctggctgttt
    14281 ttcctgtgaa agcaggtttc tggcagattt aaccagtgaa tcagtaaccg cttcccgggg
    14341 ctggccagta cgcccctgaa ccgccagcgt attcatcaga gcaaccaggg gggactggcg
    14401 cacatcagcc atcagcgtca gttgctctgt cacatcagac agcgtctgag ctttacgcaa
    14461 gtgcagactg ttcagaaaat tcagccaggc attaccaaaa tcagcaaaat agcgcgttgt
    14521 caggcgctgt ctgagtgctt ccggcgatac cggtgacggt gccttctgcc tgccatccgt
    14581 cagtacccag tccatttctt cacgacgctc attaatcacg gtgtctatcg acggcaatac
    14641 ggcctcttcc caggcctggc gggtaaacat accgggaacc acttcatccg taaaaaagag
    14701 gcgggagaca tcagtatcac cggtcatatc cgtcagacgc atatccgcaa actgatgtgc
    14761 cacccgtgcc agcattttct gatacagcat ggcatcgctg ttctgtgttc ccagatggcg
    14821 aagcagcaga gttctgctct ggctgaccag ctcttcatca gccgtcagct tccactgagg
    14881 atgagtaata atcccggagg cataatacgc cagcaacgtc gggccattag cctgccagaa
    14941 aacggcactc acaccgtcac gctgtggcca ttcccgcatg agcgtacgac tgaacctggc
    15001 gggctccata tgttgcggtt gcgccagcat cagatacagt ctgagctggt catatgcggc
    15061 ttttgccatt ttcccccgct gcgggctgtc cggaggaaga cgaataaatg tcctgagctg
    15121 ctgctcaaga tgagcagcag cggcatcacg cagtaaaaga tgtgcatttt gtgcataaag
    15181 gggcattacg accgcgagca ggtctgcatt ctgattcata ccggcccgca ggtaccatgg
    15241 cgccccgtgc tcctcccggt actgaaggcg ggccagggat ttctgcagat tcagcaacgc
    15301 ctgcagacgt tctgcaggag acagttgctg gttctgagcc ggacgaatct gttcctgtac
    15361 ttcagcaacc agactccggt ttgccagaaa ggaaaccacc attcctgttc cctgagccat
    15421 catcagtatg gccgccataa cggccagact tctgcgccag ttatgacctg tgcgggaagg
    15481 ctgtagccgg acaggtaatt gctgcactgt ctccggcaac gcctcccagc ggttatccat
    15541 tcgccagcga tgtctgacac ttcgttcacc gccgacagtt gccggactga aaacaatacc
    15601 cgccagcaac aactgacggt atggacggag taatacggat aatggctcca ccacagcatc
    15661 aatatttcgt ctgaatcgct ctgccagcga caacaaaaaa taataccgag gggcacagca
    15721 tatctgctgt atcccctgag caacaagccc ggggagcatg gcctgtagtt gtgctttcag
    15781 tttgtcagaa ctacattctg ctggcagaag gcatcccacg ggctgagtaa tacgccctcg
    15841 ctcatccgga ctctcctgca gtgaccagac atacagcggc agacgccagc caaggcgctc
    15901 acaacatgaa ctgaccgcac gggaaaccct gtcaagggca tcttctgtca gtggtgcgga
    15961 taatgtttca ctcagtccgg aagttaccca gacaatgcca tcagcaggcc ggtaacgcag
    16021 acggcgtaac gcagccagcc agtctgcgtt ttccggctgc gaggggtctc ctccccagag
    16081 aagcaatgtc ccctgctctt cctgccagaa ctgttctgtc aggcccgggg tcagcaactc
    16141 aacctcagag gcagtacctg ttatcaacag aatgcgtatt ttacgtcccc agaatctgcc
    16201 atgctggcgc cgcagagtat gacgaatatc ctgaacagta acatagcgcg gtgacgtctg
    16261 agccagtcgc tcttcatcgt caggtaacct ttgctcctta cgggcccgac gtcgatatgt
    16321 cagttcctga acatattttt tcatcacagg caggtaacag acaaagagca accccagact
    16381 cagcaatatc agcagtagta tttgatcccg gcgtgtttct gcccccagaa atgctccata
    16441 tcgggttatc agccaccaga gcgcagccac accaataaat acaatccccg gtcgtccaaa
    16501 gcgaccagac agacaggcca gtttattcat tctggtttcc tgatgtaaca gtactgataa
    16561 aatgttcttc tccggatgag aataaagtca ggagagaatc attaccaaaa cagaccagat
    16621 cggctgccag agcagtaagc agccacggag cagcaggtcc cgggataccg caccattttt
    16681 ccagtaacat acgtccggac ggttcccatc cggcaccata ctggtttcca atagtcatca
    16741 gcggtgcagc aatcttttcc cagtgataac agtcccccag tacacgagcg gtacgacagg
    16801 cagctgtctg ggtttcaagg aaaagtgtga attcatcatt aaacttgttg atatccagcg
    16861 acatcgggcg tagcagacgg gctggatgcg gcaggttata tttttgcgca acatcatcgc
    16921 tggtcaacaa aagtgcagcc agtccgtcag agtatgcatt tcccccatta agctgtatta
    16981 ccagtatcaa atccacagtc agcactggtt gtttcagacg ctcatcaacc caccccatgg
    17041 aaaagtcggg agtaacctcg atattgtcag gaacaacacg ttggggaaat aacacttccc
    17101 atgcagaaac aaaagcagat ctgatttcag aatcaggctg atcggttatc agcgtcacat
    17161 ttacagggag aacggcaggt aactgacaaa atttatccat tatctcccta agcaagacac
    17221 gtaaagaggc ttcaacctta tgcccactgt cagaaagata atctattttc tttacaaggc
    17281 cgtactgcag aggcaattca tcacaaagag tagctaccgt tattttatca ggcaaataaa
    17341 cacaactggc agtaataaca agataccttt cggcccagga ttcccactgt ttctgagcat
    17401 actccccctc ttttttaaag aacaaatgtt catccatctg gttgctccac tgccagtgtc
    17461 gcaggcaaaa caggaaaaac cagccccccg gaggagtcaa tgcaagccac cagatactga
    17521 aatcatttaa tgctttcaca tattctgatg cccggataat aaacaacaga gcaccaacaa
    17581 cagccataaa tgcccctgtc ataaaccaca acatcattga tggtttatga ggtaattctg
    17641 ttgataatga ttttgttctg ctccatccca tgggttatcc tattgcacat tcaggtgcac
    17701 tggatataac cttacaccca cacagacatt tacatccatc aatcacaaca gctcttccat
    17761 cagatatcca ttcaggagag ccctccacaa taggattagt accatgtccg gaaacaggac
    17821 aactaaccat atctccgaca agagctactt ttttaccatc gacaatcatg gttgaagatg
    17881 ccgaaataac tttaccacca tgtgtggtat tatcaccaag caatacaaaa cctttagaca
    17941 taaaactctc aaaaataaat tttactttga gtcaccagtt accgtagggg taaattttcc
    18001 ctttgataaa atctggtgta tcaccgtaat
//
LOCUS       DAZGAP010000107        18013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGAP010000107 DAZGAP010000000
VERSION     DAZGAP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.107"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(73..819)
                     /gene="cutC"
                     /locus_tag="ACHLAM_003172"
     CDS             complement(73..819)
                     /gene="cutC"
                     /locus_tag="ACHLAM_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO7854655.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(833..1399)
                     /gene="yecM"
                     /locus_tag="ACHLAM_003173"
     CDS             complement(833..1399)
                     /gene="yecM"
                     /locus_tag="ACHLAM_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7854656.1"
                     /translation="MANWHSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1615..3348
                     /gene="argS"
                     /locus_tag="ACHLAM_003174"
     CDS             1615..3348
                     /gene="argS"
                     /locus_tag="ACHLAM_003174"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO7854657.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3401..3793)
                     /gene="flhe"
                     /locus_tag="ACHLAM_003175"
     CDS             complement(3401..3793)
                     /gene="flhe"
                     /locus_tag="ACHLAM_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO7854658.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVSGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3793..5871)
                     /gene="flhA"
                     /locus_tag="ACHLAM_003176"
     CDS             complement(3793..5871)
                     /gene="flhA"
                     /locus_tag="ACHLAM_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO7854659.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5864..7012)
                     /gene="flhB"
                     /locus_tag="ACHLAM_003177"
     CDS             complement(5864..7012)
                     /gene="flhB"
                     /locus_tag="ACHLAM_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO7854660.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7201..7845)
                     /gene="cheZ"
                     /locus_tag="ACHLAM_003178"
     CDS             complement(7201..7845)
                     /gene="cheZ"
                     /locus_tag="ACHLAM_003178"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO7854661.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7856..8245)
                     /gene="cheY"
                     /locus_tag="ACHLAM_003179"
     CDS             complement(7856..8245)
                     /gene="cheY"
                     /locus_tag="ACHLAM_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO7854662.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8260..9309)
                     /gene="cheB"
                     /locus_tag="ACHLAM_003180"
     CDS             complement(8260..9309)
                     /gene="cheB"
                     /locus_tag="ACHLAM_003180"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO7854663.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9312..10172)
                     /gene="cheR"
                     /locus_tag="ACHLAM_003181"
     CDS             complement(9312..10172)
                     /gene="cheR"
                     /locus_tag="ACHLAM_003181"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO7854664.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNLHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10191..11792)
                     /gene="tap"
                     /locus_tag="ACHLAM_003182"
     CDS             complement(10191..11792)
                     /gene="tap"
                     /locus_tag="ACHLAM_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO7854665.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLESASAQSQRNYHISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(11838..13499)
                     /gene="tar"
                     /locus_tag="ACHLAM_003183"
     CDS             complement(11838..13499)
                     /gene="tar"
                     /locus_tag="ACHLAM_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO7854666.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13644..14147)
                     /gene="cheW"
                     /locus_tag="ACHLAM_003184"
     CDS             complement(13644..14147)
                     /gene="cheW"
                     /locus_tag="ACHLAM_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO7854667.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14168..16132)
                     /gene="cheA"
                     /locus_tag="ACHLAM_003185"
     CDS             complement(14168..16132)
                     /gene="cheA"
                     /locus_tag="ACHLAM_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO7854668.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16137..17063)
                     /gene="motB"
                     /locus_tag="ACHLAM_003186"
     CDS             complement(16137..17063)
                     /gene="motB"
                     /locus_tag="ACHLAM_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO7854669.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17060..17947)
                     /gene="motA"
                     /locus_tag="ACHLAM_003187"
     CDS             complement(17060..17947)
                     /gene="motA"
                     /locus_tag="ACHLAM_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO7854670.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
BASE COUNT         4213 a         5109 c         4456 g         4235 t
ORIGIN      
        1 ggcccctgtt gaaattgcag gggcctggta cgagcaagca tcatattggg cgacatgatg
       61 caacggtaaa aatcatttgg cctgatggcg ttcaatgatt cctttcattt cagcaaccgc
      121 cgccccgtct acgacataac gcgaatactc gtccgcgtgt gcatctgatg acatggacaa
      181 tccttgatta cgataacgca ttggtgaggc ttgccacgct cccgcggagc tatggacttc
      241 cagcactccg gcatcgagga agtggtgcag gttttctgca cggactcctg ctccggccat
      301 aatgattgga gcatcaccat gggcaataag ttccataatt tttgataaac cttgcagtgc
      361 gtctgatttt tgccctgatg tcagtacccg agcaacgccc aattccgcaa gattattgag
      421 tgtatttaaa gggttagcgc acatatcgaa ggcgcgatga aaagtcacgg ccagcggacc
      481 ggcagcagcc attatttttt ccattcgtgg catatcgaca ttcccgtcaa catcgagaac
      541 acccgtcacc agtccaggaa aacctaattc gcggactgtg cgcacatcct caagaatggc
      601 ggcaaactca ccgtcgctgt agcaaaaatc accaccgcgt gggcgaataa taggatgcac
      661 agggatcgtc acccgctggc gcacggattt cagcaccccc aacgacggcg ttaagccccc
      721 ctcttttggg gctgcgcata attcaactct gtctgcgccg ttttgctgcg ccgttagcgc
      781 gcattccatg ctgtaacagc aaatttccag taatgccatt tttgctcctt aattacgccg
      841 actgctcgct ggcaacgatc tcttcaatgg accacggatg aaatttaatg gtcgttttgc
      901 catcggtgac cgccagcgtc ggattcggta atcgctcatg ctcacctttt ggcgaactgg
      961 ttttaactga gatccccggc aggctgagtc cttcatcaga aagcaatgcc agcgcacggg
     1021 cattcagcgt ttctggatcg cccggcagaa cgatttcaat atgttcccaa ccttcgtgcg
     1081 ggtaacgttt ttccccgggc cacggtagct ccacaatgga aaactgccag tgcgcaacct
     1141 gtaccggttc atgcaattta aacagacaaa tcggcctgcc attgatcata ttttctgaca
     1201 aaagctcgcc gcactgttca aacccgcgac gccagcgctc agcggtggcg ttttgatggc
     1261 aacgcaaaga aatgtgatcg gcagtcagcg gagtgatatc caaccccaaa cggcgggaaa
     1321 gttcatctaa tgcgtgggta aatcgcggta aatccgatgc aatatcctgc agctcgtcga
     1381 tagaatgcca gttcgccata atcactcttc gtcttccact aaaagcgtta atttaccctg
     1441 ttgccctgcg ccaaccaacc gctgatttca cgccgcttct gatgcaatag tgaaaacgac
     1501 aatacgccac gcgcacgttg ctgacgaaaa cagccatttg cagtatactc ccgccctaat
     1561 ttctttaact ggtgcgggta atttttgctc gcttcatcaa tgtaaggtat tccggtgaat
     1621 attcaggctc ttctctcaga aaaagtccgt caggccatga ttgcggcagg cgcgcctgcg
     1681 gattgcgaac cgcaggttcg tcagtcagca aaagttcagt tcggcgacta tcaagctaac
     1741 ggcatgatgg cagttgctaa aaaactgggt atggcaccgc gacaattagc agagcaggtg
     1801 ctgactcatc tggatcttaa cggtatcgcc agcaaagttg agatcgcggg tcctggcttt
     1861 atcaacattt tccttgatcc ggcattcctg gctgaacatg ttcagcaggc gctggcgtcc
     1921 gatcgtctcg gtgttgctac gccagaaaaa cagaccattg tggttgacta ctctgcgcca
     1981 aacgtggcga aagagatgca cgttggtcac ctgcgctcta ccattattgg tgacgcggca
     2041 gtgcgtactc tggagttcct cggtcacaaa gtgattcgcg caaaccacgt cggcgactgg
     2101 ggcactcagt tcggtatgtt gattgcgtgg ctggaaaagc agcagcagga aaacgccggt
     2161 gaaatggagc tggctgacct tgaaggtttc taccgcgatg cgaaaaagca ttacgacgaa
     2221 gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa aactgcaaag cggtgacgaa
     2281 tatttccgcg agatgtggcg caaactggtc gacatcacca tgacgcagaa ccagatcacc
     2341 tacgatcgtc tcaacgtgac gctgacccgt gatgacgtga tgggtgaaag cctctacaac
     2401 ccaatgctgc cgggtatcgt ggcggacctg aaagccaaag gtctggcagt agaaagtgaa
     2461 ggcgcgaccg tcgtattcct tgatgagttc aaaaacaagg aaggcgaacc gatgggcgtg
     2521 atcattcaga agaaagatgg tggctatctc tacaccacca ctgatatcgc ctgtgcgaaa
     2581 tatcgttatg aaacactgca tgccgatcgc gtgctgtatt acatcgactc ccgtcagcat
     2641 caacacctga tgcaggcgtg ggctatcgtc cgtaaagcag gctatgtacc ggaatccgta
     2701 ccgctggaac accacatgtt cggcatgatg ctgggcaaag acggcaaacc gttcaaaacc
     2761 cgcgcgggtg gtacagtgaa actggccgat ctgctggatg aagccctgga acgtgcacgc
     2821 cgtctggtgg cagaaaagaa cccggatatg ccagccgacg agctggaaaa actggctaac
     2881 gcggttggta ttggtgcggt gaaatatgcg gatctctcca aaaaccgcac cacggactac
     2941 atcttcgact gggacaacat gctggcgttt gagggtaata ccgcgccata catgcagtat
     3001 gcatacacgc gtgtattgtc cgtgttccgt aaagcagaaa ttgacgaaga gcaactggct
     3061 gcagctccgg ttatcatccg tgaagatcgt gaagcgcaac tggcagctcg cctgctgcag
     3121 tttgaagaaa ccctcaccgt ggttgcccgt gaaggcacgc cgcatgtaat gtgtgcttac
     3181 ctgtacgatc tggccggtct gttctctggt ttctacgagc actgcccgat cctcagcgca
     3241 gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac aactgacggc gaagacgctg
     3301 aaactgggtc tggatacgct gggtattgag acagtagagc gtatgtaatc gcgttatacg
     3361 gcaaaagccg atgtcatggc agacatcggc ttttttatca ttaacgataa ttcacaatca
     3421 cttcattccg ttgtaccttt agcggcggaa ttaaccgccc accgccagac acttcccaga
     3481 taaatcgcaa cggttctatc gccggtatac cggaaaaggc cacggtggtt ccactctgcc
     3541 cttctaattc gacacaacga gattgtgaac acaagcgaac ccgcagtcct gcaggtgtcg
     3601 ggccgataag ctgataacgc cacgccacca gcgtcatcaa tcctgcagca ggttgtcgag
     3661 tagaaagagg cgctgacgac atcgactcac cacggtgatt cagcgtaacg cccacactac
     3721 tcgcctgcca catcccctcc ccggcggctt gcaccagcag cggaaacaat aatattgcta
     3781 ataaagttct catcatttac cgccaattgt cgccgtcatg cggatatgtc ggttatcaga
     3841 tagttccaga ttcgacagca ccactaactg cggcaggctg cggcgcagga agcgagacaa
     3901 taatggtcgc agcgcgtggt tcaccaacaa taccggcggc gcacccaaca tctcctgacg
     3961 ggatagcgct tcctgagttt gcgccagtaa acgatccgcc agccctggct ccagtccccc
     4021 cccgccctgc aacgcctgta gcaacaaacg ttccagcggt gtatcgaggc caataacatg
     4081 gacttcatct ttgccaggaa accactgctg ggtaatcgcc cggcccaacg ctacgcgcac
     4141 gacggcggtt aattcatgcg gatcgctttg gatgggcgca tgttccgcca gcgtttcgag
     4201 aatggtgcgc atatcgcgaa tcggcacttt ttcatcgagc agattttgca gcactttatg
     4261 cagtgtggta agcgtgacga caccaggaac gagatcttcc gtcagctttg gcatctcctg
     4321 ggcgacgcga tccagcagct gttgtgcctc ctgacgacca aacagctctg cggcatgctg
     4381 gctaatgagg tggttaagat gggttgccac caccgtgctg gcctcaacca cggtgtaccc
     4441 ctgaatctgc gcctgttctt taagcgcact ttcaatccag atagcattca ggccaaatgc
     4501 cggatcgacg gtcgcctcac caggtaacgt cccggcagcg gtaccagggt taatcgccaa
     4561 ccaacgcccc ggataagcat caccactgcc tatctccacg cctttcatca aaatgcgata
     4621 gcgggcaggt tgcagatcca tattgtcgcg aatgtgcacc accggcggca gaaaccccat
     4681 ctcctgggca aatttcttgc gaatactgcg tatacggccc agcaactcac catcctgctg
     4741 gaaatcgacc atcgggatca ggcgataacc cacttccatt cccagagaat cttccagttg
     4801 cacatcgttc cacgtcgctt cgacaacggt attattctct gccattttta ccggttttgg
     4861 ttcggcaggc gctttttgtt cacgtccgcg gatccaccag gccagtccca gcaatccggc
     4921 agtgaacagc aaaaatacca ggttcggcat tccaggcacc aggccgagta aaccgagcac
     4981 ggcggcgctt aacaacataa cgcttgggtt actgaaaagc tgattcacca tctgctcgcc
     5041 aacatcctga tcggtgctga cacgcgtaac gatgaccccc gcggcggtag aaatcaccaa
     5101 cgccggaatt tgtgccacca gaccgtcgcc aatagtcaat agcgtataac tttccgccgc
     5161 gtgtcccatg ctcataccat gttgcagcac gccgaccagc aacccaccga caacgtttat
     5221 gaccatgatg aggatcccgg cgatggcatc gccgcgaaca aacttacttg ccccgtccat
     5281 cgagccgtaa aaatcggctt cctgagtcac ttcggagcgg cgttttttcg cttcatcttc
     5341 accaatcaat ccggcgttaa ggtcggcgtc aatcgccatc tgcttccccg gcataccatc
     5401 gagaacaaag cgcgcgccta cttccgcaat acgcccggca cctttggtaa tgaccataaa
     5461 gttgatgatc acgagaatga cgaacaccac gataccgata gcgaaattgc caccaacgag
     5521 gaagtgaccg aacgcttcga ccacctttcc agccgccgcc gcgccggtat gcccttccat
     5581 caaaatgata cgggttgaag ccacattaag tgccagacgc aacaacgtgg taaacaacag
     5641 aatcgtcgga aacgcagcaa actcaagcgt gcgttgggta aacatcgcca ccagcaacac
     5701 catgatcgac aaggcaatat tgaaggtaaa cagcaggtcg agtatgaatg cgggcagtgg
     5761 cagtaccatc atcgacaaga tcaacaggat caaaatcggt ccggcaagga tctgccagtg
     5821 tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc agattactca tgggtcggtt
     5881 tctcgttaat aaaatccagg gcttccggca ccggaagatg agtaggttgt acagggcgct
     5941 gtccaccagc cagacgccag cgtttcagtt gccagaccca ggccagcact tccgccaccg
     6001 cggcgtacag ttgacccggg atttgttgac cgatctccgc atgtcgatac agcgcacgcg
     6061 ccagcggcgg cgcttcaagc gtcgggacgt tattttcagc gccaatttca cgaatgcgca
     6121 gcgcgaccag ccctgcacct ttagcgacca ctttcggtgc gctcattttg ttttcgtcat
     6181 actgcaaagc cactgagtag tgagttgggt tattgacgat aacgtccgct ttcggcacat
     6241 cggccatcat ccgccgccgc gccgcagctc gctgcatctg acggattcgc cctttgacat
     6301 gagggtcgcc ttcgctttgt ttgaactcat cacgaatatc ctgccgtgac atacgcagct
     6361 ttttcaggtg gctgaagatt tggaaaaaga cgtcaaatcc caccatcgga atgacaccaa
     6421 gcaccaccag caatgcgcac agccctacca gatccatcgc attacccatg gcggtaatgg
     6481 gggactccgc cattaagcgc atcatttgcg gccagtggtg ccagagataa aaccccgtca
     6541 cactgccgac caggatggat ttcaaaatcg ctttaagcaa ctccgcaccg gtttgtgccg
     6601 aaaacatccg tttaatgccc ggtagcgggt tgagtttgga aaacttcggc tgcaaggatt
     6661 tgccgctaaa taccaggcca cccaacataa ccggagaaat aagcgccacc agcaccacac
     6721 cgctaatcag cggcagcagc gccagcatgg cttctctgat cagcagaata atttgcccga
     6781 ggatcagatt cgggtcattg ataatactgt gatcaaaatg cagcccagcg gagagcatgc
     6841 ccgacaatcg acgggccagc gacacaccgc caaaccagat aacacacacg cccactaaca
     6901 aaatcagcag tgacgttaat tcacgtgaac gcgggatttg cccctcttcc cgcgcttttt
     6961 ctagtcggtg aggtgtgggg gcttctgttt tgtcgtcgct ctcgtcagac acgtcgccaa
     7021 tcctggaaag agtattaagc gtaaatgatg ccagagcgca aagcgttcaa tggtttgagt
     7081 aaggggcaaa acaggcggga tttagggctt ttactgccac acatcaagca tagtatgcgt
     7141 ttgtcggatg cggcgtcatc gcctgatatg acgtggttac gccacatcag gcaatacaaa
     7201 tcaaaatcca agactatcca acaaatcgtc cacctgatcc tgactggcta ccacaccggc
     7261 tttgctggta tcgacctgag gtccattaag caaactctgg ttttcacgtt ttggacgcga
     7321 ctcctgttcc gggatgtttt ccaacagcac catcaacaac tggcgttcga tctcctgaat
     7381 gacatccatc atccgcttaa taacctgccc ggtgagatcc tgaaaatcct gcgccatcat
     7441 gatttccagc agttgcgcgt tagtaaagct ggtatgcgcg gggacatctg ccagaaattg
     7501 ccgcgtatct gttaccagtt ctcgggcgtc ggcaaggtca atcggatcgg caaaccagtc
     7561 atcccaacgt tgggttaacg cttttgctga tttctccatt tgatcctgat gcggttgtga
     7621 cgcctcaaca ctgttcagcg cccgctccgc agcctgggcg gtcatctgca caacatagta
     7681 caaacgatcg cgcgcatcgg ggatggcttc cgccgcttcg gcaatggcct gatccagccc
     7741 cagttcccgc aaactgtcgc gcagcatacg cgtcaggctg ccgatgcgcg caatgatatc
     7801 gccagctgaa tgctcgtcag caggtttgat tgatggttgc atcatagtcg catcctcaca
     7861 tgcccagttt ctcaaagatt ttgttgagtt tttcctccag cgtcgcggcg gtaaatggct
     7921 tcaccacata gccactggcc cccgcttgcg ccgcagcaat aatgttctct ttcttcgctt
     7981 ctgcagtcac cattaacact ggcaatgctg acatcgcgcc atccgcacga atcgttttca
     8041 gcaattccag gccatccatg ttgggcatgt tccagtcgga gataacaaat ccataaccgc
     8101 ctgcctgcaa cttattgaga gcgtcgacac catcttccgc ttcctcaaca ttattgaatc
     8161 ctagctcttt cagcaggtta cgcactatgc gtcgcatggt ggaaaagtca tccacaacca
     8221 aaaatttaag ttctttatcc gccatttcac actcctgatt taaatacgta tcgcctgtcc
     8281 ggcactaatt tttgccagca tttgctggct tacctggcta agatcgacca cttcgcaaac
     8341 accacccata ttgatggcct cgcgcggcat gccgaacacc acgcaacttg cttcgttttg
     8401 tgcaagggtc catgcccccg cctgacgcat cgccaacatt cccaccgcgc cgtcgttgcc
     8461 cataccggtc aggatcaccc caaccgcatt acgccccgcc tgtttggcga cagaatggaa
     8521 caacacatct accgaaggcc gatgacggtt aaccgccggg ccatcgtgaa ttttgatttg
     8581 gtaatttgcg ccactacgcg ccagctccat atgccgatcg cccggcgcaa tataagcatg
     8641 ccccggcaag acacgttctc cgtcttcggc ttctttaacc ccgatctggc aaagcttatt
     8701 aagtctgtcg gcaaaagagc gggtgaaacc gggcggcata tgctgggtaa ttaacagtgc
     8761 cgggctggaa agcggcaacg gttgcagtac gtgacgaatt gcctcagttc cacccgttga
     8821 agcaccaatc gcaatcagtt tttcagagct caacaacggc cctgccttca gcgttgtcgg
     8881 tgccgacaat ggcttatgtg ctgcaaggct cgcctttgct gctgtacgca ccttttcagc
     8941 aatcatttcg ctatacgcca gcattccttc gcgaataccc agttgcggtt tggtgacaaa
     9001 atctatcgcc cccagctcca atgcgcgcag cgtgacttct gatcctttgc cggtcaggga
     9061 agaaaccatc acaacgggca ttgggcgcaa acgcattaat ttttcgagaa aatccagtcc
     9121 gtccatccgc ggcatttcaa catccagcgt tagcacatcc ggattgaatt tcttaatcaa
     9181 atcacgcgca accagcggat caggggcggt cgccaccatt tccatgtcgc tatggctgtt
     9241 gatgatttct gtcatgatct ggcgcatcag tgccgaatca tcgacagata acaccctgat
     9301 tttgctcatc gttaatcctt acttagcgca tacaccgtct gaccacgcag cgtgaagcgg
     9361 cgctcaaggt ggctaaagtt ttcagagtga cccgcaaaca gcaatccgtc gggtttaagg
     9421 agcggaacaa agcggcgcaa aatctcctgc tgggtagttt gatcgaagta gatcatgacg
     9481 ttacgacaga agatcgcatc aaacggcccc ggcacggtgt actgtttcgc cagtaaattc
     9541 agcggggcaa aatcaacata gttcgccagc tcctgacgca cgcgtaccag cccttcatgc
     9601 ggtcccgtcc ctcgcatgaa ataccgttgc agttgctgcg gcgtcaggtt tttcaactct
     9661 tcatggcgat aaataccgct tctggctttt tccagcactt cggtgtcgat atcgctggca
     9721 aacactttcc agcgtccggg cacggtgccc aatgtgtcag ccagcgtcat cgcaatgctg
     9781 tacggctctt cgccggtcga agccgccgcg ctccatacgc gatactcgcc agaacggcga
     9841 cgtgcgtgat ccgcgagcag aggaaaatga tgcgcctcac ggaaaaatgc cgtcagattc
     9901 gtggtcagcg aattgataaa cgcctgccac tcaccgctgt gcagattaga ttccagcaag
     9961 ttcaggtaat gaccgaaatc cgtcagtccc agcgaacgca aacgacgaac cagtcggttg
    10021 taaaccatgt cgcgtttatg gtcggccagg acgatcccgg ctcgttgata gatcaattga
    10081 cttatccgcc gaaaatgcgc gtcggaaagc gccaggcgct cggtcatctg taacaataaa
    10141 gacgtttgcc cacagggcag agatgaagtc atagcgcctt ctcaatcact tcaggatacc
    10201 actggcgcaa tttgtaactg cgccgactca tgtcgtgcta cttcatgctc ttcaagggta
    10261 aataccgcca cgcgcgacga aagatggtcg gcctgattcg ccagttgttc tgttgccacc
    10321 gccgcttctt ctaccaggga ggcgttctgc tgtgtcacct gatccatctg gctgacagcc
    10381 tgtgcaacct gctcaatccc ccgccgttgt tcttccgagg ccgaagcaat ttctcccata
    10441 atgtcgttca cgcgggtcac cgaactgaca atatcagtca tggtcgcggc ggcgttattc
    10501 accagtttcg aaccctgctg gacacgattg actgactctt cgatcagccc tttgatctct
    10561 tttgctgcct gggcgctacg gctggcaaga ttgcgcactt cacctgccac taccgcaaaa
    10621 ccacgcccct gctctccggc gcgagccgct tccactgccg cattcagggc cagaatattg
    10681 gtctggaaag caatgccgtc gataacgctg ataatgtcgc caattttttg cgagctggta
    10741 gcgatctcct gcatggtgtg agtcatggta ctgacctgaa caccgcccgc ctgcgccgtt
    10801 gtcgcggcat tttttgccag ttccgacgcc tgtcgtgcgt tatcggcgtt ttgccctacc
    10861 gtggcggtta attgctccat actggcggcc gtttgtgcca gcgatgccgc ctgctgttcg
    10921 gtgcgacttg agagatcgtt attgcctgcg acaatctccg cgataccaat gtgcatctcc
    10981 tggcttccct tacgcacatc acttaccgtc ccacgcaaag cctgctgcat ggtcttcagg
    11041 ctggcaaaaa tggcggtgat ctcattgcga ccatacaccg caatcggacg tgccagatta
    11101 cccgcagcaa tgctgtcaaa atggctaccg ataatggcca gtggttgaac aatcatcttg
    11161 cgcgtccacc acagcgcact gctgatgtag atcgccgcaa caataatcat gctgataaac
    11221 accagcgccg aaatgtgata gttacgctgg ctttgcgcac tggcactttc cagcacatgg
    11281 ttgatctcca gctgccaggc ctcaaagtta acgtcaaacg cattctgtga ttcctgcacc
    11341 ggcgcagtga ggaaatccga aagttgatta ctttcaagcc aggtcgcctg atgttcgaga
    11401 tcgttgtgcc agcgggcaaa acttttctcc gtctcttttt gcaacgccct gacgtgctcg
    11461 ttgcccgcag tcatcgccat aaaactttta aacagcgtgg tggattgcgt cagactggcg
    11521 cgcgccgtcg tcatcaacgt tttaatgtca tccgccggat agctaagcgc cgtcagagtg
    11581 cccgctttgt tcagcgcggt actggcctgt aacattaccg cccgagtttg cgctaatgcc
    11641 gcacgttgct gattgctctg ctcgacctga ttcagtcgtt gcaaatcgtc gcgaaatgcc
    11701 caaaaagaca tgccgttact gccaatctgc aagatcccgc agagaatcaa aattaaaaac
    11761 agcgtggtcg aaattcgaat acgattaaac atcaacgctc cccatcaggc ggcaatgacc
    11821 gcgttagtaa atactcgtca aaatgtttcc cagtttggat cttgttcagc aattcgcagt
    11881 cgtggctgtg ccggtggttg ctcactggca ggacgggatg gtgtttgcgg tttattggtg
    11941 agtgggctgg ctgccagacg gaacgcggaa accgcttgcg ttaaacgact cgcctgttct
    12001 tccagcgcag ctgcggcggc agctgattcc tgcaccagcg atgcgttctg ttgcgtgacg
    12061 cgatccattt ccgaaaccgc caatgcgact tgatcgatgc cacggctctg ttcatccgat
    12121 gccgatgcaa tctcgcccat aatgtcagtc acgcgggtga cagcattgac gatattgttc
    12181 attgtttccc cggcgctttc gaccagcacc gaaccggtat caacgcgcga gacggagtct
    12241 tcaatgaggg ctttgatctc ttttgccgcc tgggcgctgc gactggcaag attacgcact
    12301 tcgcccgcca ccacggcaaa accacggccc tgttcacccg ctcgcgcggc ttcaacagcg
    12361 gcattcagcg cgagtatatt agtctggaag gcaataccat cgataacgct gataatgtcg
    12421 gcaattttct tcgaactatc ggcgatctca tgcatcgttt tcactacgcc atccaccact
    12481 ttgccgccgt gctgggcggt gtcggaggca ctttgcgcca gttgcgaggc ctggcgggcg
    12541 ttatcggcgt tttgcttcac tgtcgcggtg agctgctcca tgctggcggc agtttcttcc
    12601 agcgcggatg cctgctgttc cgtacgggag gaaagatcgg tgttgcccgc cgcaatttca
    12661 cgggtaccgg catagatggc atctgaacct tcacgcacat gagtgacggt gtcagtcaaa
    12721 gagcgttgca tatgtgaaac gctctgcgcc aggtcgccca tttcactgcg cccgtcaatg
    12781 gtcagggtat tcgccaggtt accaccggcg atttcgcgaa tgtgagcaat aatttttgcc
    12841 agcggtgtaa gcaacatacg gcgaatgccg taccacgcca ccagcagaat caataccacc
    12901 accagcgcga taaccgccag ttgccactgg gcaaatcggt aatcatctgc gttgtcagtg
    12961 acgatatcgc gatacagttt ttcactgctg agggcgtact gagcaaacgc ttcgcccatt
    13021 gcattttgca ttccctgggt tggctgagcg aaataagctc cagtattgcc ataatcaaga
    13081 taatcaatca gttcagttaa cgctgtgtga tagtttttat atttttcatc aatattacga
    13141 ctggtagcga ccatttcagg taacggtgcc atgcttttga attttttata atgagtcgct
    13201 gcctgggcca atgttttcct ggcgctatcg agcaattcaa ctttggcatt actttgctga
    13261 ttggatgaat ccatcatcat ccgcaccgct gaacgactca ggttaatgcg cgtttgcagc
    13321 attaaatccc atgttgacgt cagctcgccc tgctgttccc gtaattgatt ggaaaccaca
    13381 aagctcttct ggctatggtg aagggaagaa aaaaacagac tgccggaaat aagctgtaac
    13441 agtgcgaata cccccagcac cattaccaac agcgtgacta cgcggatacg gttaatcata
    13501 aggcaccttc ctgaaaacaa gttgatctcg ttatcggcaa ggaaggggga aactttattg
    13561 ctgatgccac ccgccgcgaa actgaaataa aaaacccgat gcgcagatca tcgggttcat
    13621 tgcaattgag gaaatcggga gaattacgcc acttctgacg ccgcgctatc taatagcgcc
    13681 atctcttcgc tgttcagcag tttttcgatg ttcaccagaa tcaacatacg gtcgcccagt
    13741 gcacccagtc cagtgagata ttctgttgaa agcgtcacgg caaattccgg tgccggacga
    13801 atttgctccg ccgtcaatga aagcacgtct gagacgccgt caaccacgat gccgaccacc
    13861 cgctgtccga gattcaggac gataactacc gtgttgtcgt tatagtccac atccacctgg
    13921 ctgaacttaa ttcgtaagtc cacaatcggc acaataacac cgcgcagatt cgtgacgcct
    13981 ttgataaacg ctggcgtgtt cgcaatccgt gttacctgat cgtagccacg gatctcctgc
    14041 actttcagaa tatcaatacc gtactcttca tcaccaaggg taaataccag aaattcctgg
    14101 cctgacggct cgctggccag ctttgttaca ttcgtcatac cggtcatatt gttacctttt
    14161 tactcattca ggcggcggtg ttcgccatac gttgttcgcg gtttatcgcc tgcaaggcgg
    14221 agacatcaac aatcagtgcc acgctgccgt cgccaagaat ggtcgcagca gaaatgccgg
    14281 ggactttgcg atagttactt tcaaggtttt taaccacaac ctggtgttga ccaattaatt
    14341 gatccaccag caaggcgtag cggcgaccgc cactttgtaa gatcaccaca attccctggg
    14401 tagcttcggt tttcgcgccc gcgacgttga acactttcca cagttcgacg atgggcagat
    14461 attcaccccg cacttccagc acccgctcgc cgccagccag tggatgcaga tcggcttcac
    14521 ggggttgcag tgattccatg acagcattca gcggcagaat gaaaacttca tccgcaacgc
    14581 gtacggacat gccgtcgagg atggccagcg tcagcggcag taaaatgcgg atcgtcgtgc
    14641 cagtaccctg cttcgactgg atttcgacat gaccgcccat ctcctggata ttacgtttaa
    14701 cgacgtccat gccgacgccg cgcccggaga cgtcggtgac ctgctccgcc gtggagaagc
    14761 caggggcaaa tatcagcatc gcgacttcgt cgtcgctcat gttttcgctg acggtcagac
    14821 cttgcgaggc cgcttttgcc agaattcgct cacggtttag ccccgcacca tcgtcggtca
    14881 cttcaatgca aatgttgccg ccctgatgtt cggcagacag aattaaattt ccgacgctgt
    14941 ttttacctgc ggcgagccgt ttttctggca gttcaatacc gtgatcgagg ctattgcgta
    15001 ccaggtgggt cagcgggtcg ataatgcgtt ctatcaggct cttgtcgagt tcagtggaac
    15061 tgcccaccag cgtcagttct acctgcttgc cgagttttcc cgcaagatca cgcaccagcc
    15121 ggggatagcg actaaagaca tactccatcg gcatcatgcg aatcgacatc actgattcct
    15181 gcaaatcacg ggcgttacgt tgtaactgcc ccatgctggt aatcaaatca ccatgattaa
    15241 ccgggtccag ttcactagaa cgctgggcaa gcattgactg ggtgatgacc agctcgccga
    15301 cgaggttaat taattgatca accttttcta ccgctacacg gatgctggtg gattcactgc
    15361 tgcgcgtcgt tttttcccgc tccacgcggc cggttggcgc ttgttcggct gccagtttaa
    15421 gcactggtgg gggggatatt ttgggcgaga cttctactgt ttcaaaggta atctgatcgg
    15481 cttcaatcac aaaacagagt accgctgtga tgtcatcttc ggcgatgtcg cccggtaata
    15541 ttgccgagag agaatccgcc cctttcacca catcagttaa cgttgtcaga tgccccagct
    15601 cttcttccag caggtcgact tccccggcct tcaggcgcga aaggataatt cgtcgcggcg
    15661 actgatcgcg gctctgctca tcttgcggtt cacttttggc aaccacactt aatcgggtca
    15721 ctgcagatgg cgtttcgcct ttcgcttcta atgccagttg acgcaaggcc tggcagatat
    15781 aatcgaagct ggcggcatcc ggctcttgcg actgtttata agcgtcgagc tgttcttgca
    15841 tgatgtcctt cgtttccaaa aacagattga taatgtcggt gttgagttgc atctcacctc
    15901 gtctggcttc gtcgagcagg ttttccatca gatgcgtggt ttcctgcaaa acgctgaagc
    15961 caaaggttcc tgcccctcct ttgatcgagt gggcagcccg aaagatggca ttcaattgtt
    16021 cggcatctgg cgcttccggc tgcaaaacca gcagatgctg ctccatgtca gccaacagtt
    16081 cgtccgcttc atcaaaaaat gtctgataaa aatcgcttat atccatgctc acgctgtcac
    16141 ctcggttcgg ctgatggcat tgtgggaaca ctgacctgtg gtgcaacctc aggtttttcc
    16201 agggcgctta ctggctcatt ctggctttcg gcgttttcat gcaaaatggc ctgttcggct
    16261 tgtttgttca gtaccagcag gctgatgcga cggttgacgg catcatcagg tccgcgatcg
    16321 cttaagcgca tcgttgccgc catgccgacg acacgtaaca ctttgccgcc attcaaccca
    16381 ccaaccatca gttcgcggcg ggatgcattg gcacgatcgg cagaaagctc ccagttgctg
    16441 taacctttct caccgctggc gtagggaaaa tcatcggtat gacccgagag gctaattcgg
    16501 ttgggaatac cgttcagtac gggcgcgatg gcgcgcagaa tgtcgcgcat atagggttcg
    16561 acgtcggcac tgccagttct aaacatcggg cgattctggc tatcgatgat ctgaatacgt
    16621 agaccttcct ggaccagatc gattttgaga tgtggacgta acgcccgcag tttcggatcg
    16681 gactcaatca actggtcgag atcgccacgc aatttccgca atcgactttg ctccatgcgt
    16741 tttttcagct cttcgatgtt cggctgctta ttcacttccc cctggctttg ggtgtaatca
    16801 tcacccccac cgggaattgg gctttcacta ttagaaatgc gatcgccgcc cgtaaccgca
    16861 gtcgccagtg gagtccggaa gtactccgca atctgaatca gctcttttgg gctggagatg
    16921 gagattaacc acatcaccag aaaaaaggcc atcatcgcgg tcataaagtc ggcataagca
    16981 atcttccacg atccatgtgc tgccccgtgg cttttggctt tgcgtcgttt gacgacaata
    17041 atcggatgcg cttgattctt catgcttcct cggttgtcgt ctgttgttgc ggatttttca
    17101 ccgcacgcac atgctcttcc agttcaataa acgacggacg ttcgctggaa tagagcgttt
    17161 tgcgaccaaa ctcaacggcg ataggcggtg cgtaaccgtt cagattagat agcagtgtga
    17221 ctttgacgca ctgcatcatt ttgctggttt cggcgctttt ctgacgtaaa acactcgcta
    17281 atggggaaat aaatccgtaa gccaataaaa taccgaggaa agtccccacc atcgcatgtg
    17341 cgataagcgc ccccagctcg gcggcaggac gatcggctga acctaacgcg tgaacgaccc
    17401 ccattacagc cgcaacaata ccaaacgccg gaagtgagtc accgaccagc gccagactgt
    17461 tcgccgggac ttctgcctcg ctttcgtgcg tctcaatctc ttcatccatc agagcttcga
    17521 tttcgaaggt gttcatgtga ccgctgataa tcaggcgcag ataatcgacg ataaattcaa
    17581 gcatgacgct atccgcaagg atgcgtgggt agctggcgaa gatctcgctc tcacggggat
    17641 tttcaatatc acgttccagc gaaaacatcc ccatctgccg cgatttcgcc atcaaccgat
    17701 aaagcagagc cagcagatcc atatacattg ctttggtgta tttggagcga cgaaacagca
    17761 acggcagcgc cttcagcgtg cctttaatcg ctttgccatt attgccgacg ataaacgacc
    17821 caatccctgc accggcaata atcaccagtt cagcgggttg atagagtgct ccaaggcttc
    17881 caccggtcat caaataaccg ccgaaaactg taccgagaac aaccaggtaa cctaataaga
    17941 taagcacgac atcatccttc cactgttgac catgacagga tgttcagtcg tcaggcgtta
    18001 acacgcgata ggg
//
LOCUS       DAZGAP010000108        17986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGAP010000108 DAZGAP010000000
VERSION     DAZGAP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.108"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(95..2542)
                     /gene="glgP"
                     /locus_tag="ACHLAM_003188"
     CDS             complement(95..2542)
                     /gene="glgP"
                     /locus_tag="ACHLAM_003188"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO7854671.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2561..3994)
                     /gene="glgA"
                     /locus_tag="ACHLAM_003189"
     CDS             complement(2561..3994)
                     /gene="glgA"
                     /locus_tag="ACHLAM_003189"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO7854672.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3994..5289)
                     /gene="glgC"
                     /locus_tag="ACHLAM_003190"
     CDS             complement(3994..5289)
                     /gene="glgC"
                     /locus_tag="ACHLAM_003190"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO7854673.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5307..7280)
                     /gene="glgX"
                     /locus_tag="ACHLAM_003191"
     CDS             complement(5307..7280)
                     /gene="glgX"
                     /locus_tag="ACHLAM_003191"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO7854674.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7277..9463)
                     /gene="glgB"
                     /locus_tag="ACHLAM_003192"
     CDS             complement(7277..9463)
                     /gene="glgB"
                     /locus_tag="ACHLAM_003192"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO7854675.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVRTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9736..10839)
                     /gene="asd"
                     /locus_tag="ACHLAM_003193"
     CDS             complement(9736..10839)
                     /gene="asd"
                     /locus_tag="ACHLAM_003193"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7854676.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11031..11624
                     /gene="yhgN"
                     /locus_tag="ACHLAM_003194"
     CDS             11031..11624
                     /gene="yhgN"
                     /locus_tag="ACHLAM_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO7854677.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11681..13021)
                     /gene="gntU"
                     /locus_tag="ACHLAM_003195"
     CDS             complement(11681..13021)
                     /gene="gntU"
                     /locus_tag="ACHLAM_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7854678.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13025..13552)
                     /gene="gntK"
                     /locus_tag="ACHLAM_003196"
     CDS             complement(13025..13552)
                     /gene="gntK"
                     /locus_tag="ACHLAM_003196"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO7854679.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13691..14686)
                     /gene="gntR"
                     /locus_tag="ACHLAM_003197"
     CDS             complement(13691..14686)
                     /gene="gntR"
                     /locus_tag="ACHLAM_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO7854680.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14910..15605)
                     /gene="yhhW"
                     /locus_tag="ACHLAM_003198"
     CDS             complement(14910..15605)
                     /gene="yhhW"
                     /locus_tag="ACHLAM_003198"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO7854681.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15728..16765)
                     /gene="yhhX"
                     /locus_tag="ACHLAM_003199"
     CDS             complement(15728..16765)
                     /gene="yhhX"
                     /locus_tag="ACHLAM_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7854682.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17099..17587
                     /gene="yhhY"
                     /locus_tag="ACHLAM_003200"
     CDS             17099..17587
                     /gene="yhhY"
                     /locus_tag="ACHLAM_003200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7854683.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17824..>17986
                     /locus_tag="ACHLAM_003201"
     CDS             17824..>17986
                     /locus_tag="ACHLAM_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO7854684.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNSITNTGLG"
BASE COUNT         4445 a         4858 c         4480 g         4203 t
ORIGIN      
        1 tacatcaaag ttattgttta ttgtgtatag aaataccgtg agttccactc tgtattactt
       61 cagcggaaca cacggtattt agccatattt gaacttacaa tctcaccgga tcaatatgcc
      121 agatatgatc ggcgtactct ttgatcgtac ggtcagaaga gaagtagccc atattggcaa
      181 tgttcagcat cgcttttgcg gtccactctt cctgacgctc gtagagttcg tcgactttat
      241 cctgacaatc gacatagctg cgataatccg ccagtacctg gtagtgatcg ccgaagttga
      301 tcagcgaatc gaccagatcg cgatagcgac ccggatcttc cggactgaat acaccgctgc
      361 cgatttgcgt cagcacctga tgcagctcct catctttctc gtagtattca cgcggtttgt
      421 agccctgacg acgcagttct tccacttctt ccgctgtgtt accaaagata aagatattgt
      481 cagcaccgac atgatccagc atctcgacat tcgcaccgtc cagcgtaccg atagtcagcg
      541 cgccgttaag cgcaaacttc atgttactgg tgccggaagc ttccgtcccc gccagcgaaa
      601 tctgttcaga cagatcggcc gccggaatga tcaactgcgc caggctaaca ctgtagttcg
      661 ggatgaacac gactttcagc ttgtcgccaa tctgcggatc gttgttgatc actttcgcta
      721 cgtcattgat caaatgaata atgtgcttcg ccatgtaata ggccgaagcc gccttaccac
      781 caaaaatatt cacgcgcggt acccacttcg catccgggtc ggccttgatg cggttatagc
      841 gggtgatcac gtgcaacaca ttcatcaatt gacgtttgta ttcgtgaata cgtttgattt
      901 gcacatcgaa cagcgctttc ggatttacca ccacattcag ctgctgggcg atatactccg
      961 ccagacgctt tttgttctcc agcttcgcct gatgcaccgc gtgattaacc attgggaaat
     1021 cacagtgttg ttgcagatca ttaagcaggc taaggtcggt gcgccagttg cggcccaggt
     1081 gttcgtccaa cacggctgaa agcgatgggt tcgctaccgc cagccagcga cgcggcgtca
     1141 caccgttggt gacgttggtg aaacgacccg ggaagatttt cgcaaagtcg gcaaatagcg
     1201 attgcaccat caggttagag tgcagctccg atacaccgtt aactttgtgg ctcaccacaa
     1261 ccgccagcca ggccatacgc acacgacgac cgttggattc atcaatgatc gacgcccgtc
     1321 ccagcagatc ggtatcgttc ggatactgtt cctgcaaggt tttcaggaaa tagtcgttga
     1381 tttcaaagat gatctgcagg tgacgcggca gaattttacc cagcatatcg actggccagg
     1441 tttccagcgc ctcgctcatt agcgtgtggt tggtgtagga gaagacctga caacacactt
     1501 caaacgcgtc gtcccagcta aattggtgct catcgatcag cagacgcatc atctcaggaa
     1561 tcgacagcac cggatgagta tcgttgaggt gaatcgcaat tttatccgcc aggttatcgt
     1621 aggttttatg caactgataa tggcggctta aaatgtcctg aatggtcgag gaaaccagga
     1681 agtattcctg acgcagtcgc agctcacgcc cggagtaggt ggagtcgtcc ggatacagta
     1741 cgcgagatac gttctcggag tggtttttat cttccactgc cgcgaagtag tcgccctggt
     1801 tgaatttacc gaggttaatt tcgctactgg cttgggcact ccacaaacgc aacgtgttgg
     1861 tcgcgtcggt gtcgtaacct gggattatct gatcgtaagc tactcccaga atctcttcgg
     1921 tttcaatcca gcgcgttttt ttaccttcct gctgaatgcg accgccaaaa cggactttat
     1981 agcgcgtgtt gtggcgtttg aattcccacg ggttaccgta ttccagccag tagtcaggcg
     2041 actctttctg gctaccgtta acgatgttct gcttgaacat accgtagtca tagcggatgc
     2101 cgtaaccgcg ccccggcaac cctaacgtcg ccagagaatc gaggaagcaa gccgccagac
     2161 ggcccaggcc accgttaccg aggcccgggt cattttcttc atcaatcagc tcttcgagat
     2221 ttaatcccat cgcttccagt gcgccctgta catcttcgta aattcctagc gacaacatgg
     2281 cgttggaaag cgtacggcca atcaaaaact ccatcgacag gtagtaaacc tgacgagttt
     2341 cttgcgacaa ctgggcacgg tttgagcgta accagcgctc cacgagacga tcgcgcacag
     2401 caaataacgt tgcgttcagc cattcatgtt tattggcgac gaccggatcc ttgccaatcg
     2461 taaacatcag cttgtaagcg atagagtgct taagagcttc tacgctaagc gtgggcgatg
     2521 aatatgtaaa cggagcattc atataggcgt ttcctgaaaa ctatttcaag cgatagtaaa
     2581 gctcacggta cgacttcgcc gcgacctgcc agctaaaatc cattgccata gcctgacgtt
     2641 gcacaaaccg ccacagtgaa ggacgggacc acagtacaaa agcacgtcga atagcccgta
     2701 acagcgacca ggcattacta tcttcaaaga caaacccact ggcgacgcca tctgcaaggt
     2761 tctcaagaga acagtcagaa accgtatcag caagcccacc ggtgcgccgc actaacggca
     2821 gcgtaccgta cttcaatcca taaagttgcg ttaagccgca cggttcaaaa cggctgggca
     2881 ccagaatgac gtccgcgccg cccataatgc gatgcgaaaa tgcttcgtga tagccaatct
     2941 gaacgcccac ctggccgggg tattccgctg ccgccgcaag gaaaccttcc tgcagcaccg
     3001 gatcgcccgc gccgagtagc gccagctgcc cgccctgctc cagaagacca ggtaaggctt
     3061 ccagcaccag gtcgagacct ttctggctag tcagacggct caccactgca aaaagcggca
     3121 ctttatcgtc aaccttaagc cccattgcga tttgtaactg gcgcttattt tccgctttat
     3181 cttccaacgt atcgcgggtg taacgcgagg ccaacagtaa gtccgtctct ggactccaga
     3241 ttttctcgtc cacgccgttc agtacgccgg aaagacgccc ttcacggtga cgctgttgca
     3301 acagaccttc cataccgtag gcaaactgcg gttcggtgat ctcgcgagcg taggttgggc
     3361 tgactgccgt aatgtgatcg gcatagtaca gaccggcctt cagaaaagag atttgtccgt
     3421 tgaattccag cccatgaata ttaaagaatg accatggcaa ttggatgtca ttcatgtgat
     3481 gtgcataaaa catgccttga taggccaggt tgtgcacagt aaacaccgac ttcgccggac
     3541 gcccgcgcgc cgccagatac gcaggcgcaa ggcctgcatg ccagtcgtgc gcatgcacca
     3601 catcaggacg ccagaatggg tcaagcccgc tggccatttc tgccccaacc caccccagca
     3661 gcgcaaaacg caatacgttg tcggtatagg caaataagtt ggtatcgtga tacgggcttc
     3721 ccggacgatc atagagatgc ggcgcgtcaa tcaggtatat gccaaccccg ttgtaatgac
     3781 cgaacaacag cgtgatatgt ccggcgaagg tatcacgacg ggatactacc tgcgcatcgg
     3841 tcacgccacg gcgaatatcg ggaaatgcag gcaacagtac gcgagcgtca acgccgtctg
     3901 cgatttgtgc tgcgggtaat gccccaataa catcagccag accgccggtt ttaagcagcg
     3961 ggaacatctc tgaacataca tgtaaaacct gcattatcgc tcctgtttat gccctaactt
     4021 ccgtagcatt tcgcgcgtta ccagcacgat gccttcttct gaacgataga aacgacgtgc
     4081 atcttcctct gcgttttcac caatcaccat gccttccgga ataacacaag cacgatcgat
     4141 gacgcagcgg cgcagacggc acgagcgacc tacccatact tccggtaaca atacggcgga
     4201 atcaatgttg cagaatgaat tcacgcgaac gcgcgagaac agaacggact gcaccaccac
     4261 cgaaccggag atcacacaac cgccggatac cagtgagtta agggtcatcc catggctacc
     4321 ggagcgatcc tgcacgaatt tcgctggcgg taatgattca ttgtaggtgc gaattggcca
     4381 attgcgatcg tacatatcca gttccggcac cacagaggcc agatcgaggt tcgctttcca
     4441 gtaagcttcc agcgtaccca catcgcgcca gtacggctcg gcatccgggt cggattgtac
     4501 gcaagagagc gggaacgggt gcgcataggc cagaccggct tcggtgatct tgggaatcaa
     4561 atctttgcca aagtcgtggc tggagttctc atcgcgatcg tcttcttcca gcagttcata
     4621 cagatagtcg gcgtcaaaga cgtagatacc catactcgcc agagatttgc tcggatcgtt
     4681 cggcattgac ggtgggttag caggtttttc aacgaattcg ataattttat cgttctcatc
     4741 aaccgccata acgccaaatg cggaggcttc ttcaatcggt actggcatac aagcaacggt
     4801 gcaacgtgcg cctttttcga cgtggtcgat aagcatacgc gagtagtctt gcttgtagat
     4861 atggtcgccc gccaggatca ccacgtattc cgctttataa cggcggataa tatcgaggtt
     4921 ttgggtaact gcatctgcgg tgccgcgata ccagttttcc cctttcattc tctgctgtgc
     4981 tggcagcaga tcgacaaact cgttcatttc ttcattgaag aatgaccagc cgcgctgaat
     5041 gtgctgcacc agagtgtggg actggtactg ggtgatcacg cccatacgac ggatcccgga
     5101 gttgatgcag ttagacagcg caaagtcgat aatgcggaac ttaccgccga agtgtacggc
     5161 cggttttgct cgcttattgg ttaaatcctt caggcgggta ccacgtcctc ccgccagtat
     5221 cagggcaaca gatttcaatg gcagctggcg cgccaacatt aagtgatcgt tcttctctaa
     5281 actaaccatg actaactcct tttttatcat ctctggaaca cacacaatcc gtgtgcaggt
     5341 ccctgccaga cagccgtaat cactgggtta tcctctccag cgaatggggg aatggcgtgc
     5401 cactccccag caggtaaaac aatctctgtt acctcaagcg tggcgttaat tgcgatcaaa
     5461 aagcgatccg agagcagaat ttgcagctgt ttcggcccgt tttgccactc atccgtgctt
     5521 aaaggttgag catatcgatt tagccaacgg acattgccgt cgccttcttc ccaccagcga
     5581 ttctccacca aagcgggaat gcgcttgcgc agatggatta acgcggcggt aaatgcggtt
     5641 aaaccactgc ttgcctgcga ccagtccaac caggttaatt ggttatcctg acagtaggca
     5701 ttgttattgc catgctggct gtgaccatgt tcgtcaccgg ccagtaacat cggcgtaccc
     5761 tgggagagca acaacgttgt taacagggcg tgaatgctgt cgcgacgccg ttcaaccagg
     5821 tcaagagtac cgcctaaccc ttctttacca tgattgttac tgtaattgtt gttggtcccg
     5881 tcgcgatttt cttctccgtt tgcttcattg tgtttatggt tgaagcaaac gcagtcgcga
     5941 agcgtaaaac cgtcatgcgc ggtgacgaga ttaatcgcgg cactcggcag acgaccatta
     6001 cgtttaaaaa catcgctgga ggcagcaaaa cgcccggcaa acgcccccag aggcaaatca
     6061 taatgaagcc agaaacgacg ggcagcatcg cggaaatgat cgttccactc ggcaaacagc
     6121 ggcgggaaat ttcccacctg ataaccacca ggagcaatat cccacggttc agcaattaac
     6181 ttcacctgcg agagcaccgg gcagttctgg atagcggtaa acaacggcgc atcctgacgg
     6241 aactctggcg tacggcccat gactgccgcc agatcaaagc ggaaaccatc gacgtggcag
     6301 gtttctaccc aataacgcag gcaggcgctg gcataatcca ccaccgccgg atgactcaaa
     6361 ttgagcgtgt tgccacaacc ggtccagttg tgataatcgc cgtcttctct tatccaataa
     6421 tagctacggt tgtcgatccc acgcagagag aataacgggc cgtcgaggtc cagttccgca
     6481 ctatggttga gcacgatatc aagaatgact tcgatacccg ctttatgcag tgctttgatt
     6541 gcatcgcgaa actcatccag cgccgtttct ggggagcagg catacgccgg atgcagcgca
     6601 aacatcgcca ccgggttata accccagtag ttacttagac ccatacgttg caggcgtggt
     6661 tcgctggcaa actgcgccac cggcagcagt tccagcgcgg taatgcccaa ctgtttcaaa
     6721 tagttgatca tcaccggatg ccccagggct ttataagtgc cacggatctc gaccgggatc
     6781 tccgggtgca ggtacgttaa tcctttgaca tgggcttcat aaatgatggt actgccccac
     6841 ggcgtgcgcg gcggagcatc atcctcccag tcatagtgat caaccaccac tacgcatttc
     6901 ggcgcaatgg cggcgttgtc gcgatagtca ggttcattat gaccggcgtg cagcagcggg
     6961 ttatctttaa attccccgtc aatttgccgc gcgcaaggat caatcaacaa cttcgccggg
     7021 ttaaagcgat gcccctcggc gggttgccag gggccatgaa cgcgataacc ataacgcaaa
     7081 cccggacgcg catccggcag ataaccgtgc caaatgtcgc cactgtgccc tggcaagtca
     7141 tagcgatgtt cctggccatt ggcgtcaaag acacacagtt ctacccgctc ggcatgagcg
     7201 gagaaaagtg tgaagttgac gccctgaccg tcgtaatgcg cgccgagggg tgcgggtttg
     7261 ccaatggcga gttgtgtcat tctgcctccc gaaccagcca gatagtggcc agcggtggta
     7321 gtgtcaggct taatgaatgc tgacgaccgt ggctggcaat ctcatcgctg tgtaccgtgc
     7381 cgccattgcc gacattactg ccgtgatagt gcatggaatc ggtattgagg atttcacgcc
     7441 atttgcccgg ctggttaatg ccgaagcgat aatcatgacg cggcaccgga gtaaagttac
     7501 tggcaacgat gatttcgtta ccctctttat cgcgacgcac aaagatcagc accgagcgtt
     7561 ctttgtcatc caccaccagc cattcaaaac cgtacgggtc aaaatccagt tcatgcatcg
     7621 ctttatggtg gcggtaggtg aggttcagat cgcgcaccag acgctggaca ccgtggtgcc
     7681 agttatcgcc gccttccaac agatgccagt cgaggctggc gtcatggttc cactcgcggc
     7741 cctgggcaaa ttcgttcccc atgaacagca gtttcttgcc cgggaatgcc cacatccagc
     7801 cgtagtaggc gcgcaggtta gcgaatttct gccatgcgtc gcccggcatc cggtcgagaa
     7861 tcgatttttt accgtggacc acttcatcat gcgacaacgg caggacgaag ttttcagtgt
     7921 agttgtagag catcccgaag gtcagtttat cgtgatgata ctgacgataa accgggtcga
     7981 gcttcatgta gtccagggtg tcatgcatcc agccgaggtt ccacttgtac cagaagccca
     8041 gaccgcccat atcctgcgga cgagaaacgc cagggaaatc ggtagactcc tcagccattg
     8101 tcaccgcacc ggaaacctgc tcaccaagaa tacggttggt attacgcaag aattcaatcg
     8161 cttcaagatt ctcgcgcccg ccaaattcgt tcgggatcca ctccccctct ttacggctgt
     8221 agtcgcgata aatcattgac gctaccgcat cgacgcgcag cgcatcaata ccaaaacgtt
     8281 caatccagta aagcgcgtta ccgacaagga agttactgac ttcacggcga ccatagttgt
     8341 agatcagcgt gttccagtcc tgatgatagc cttcgcgcgg atcgctgtgt tcatacaagt
     8401 tcgtgccatc aaattcggca agcgcaaagt catcggtcgg gaagtggcct ggcacccagt
     8461 cgagaatcac gttcagacca gctgcgtgtg cggcatcaat gaaataacgg aagtcgtcgc
     8521 gagtaccaaa acggcgggtc ggtgcataca gaccggttgg ctgataaccc caactgccat
     8581 cgaagggatg ctcgttaatg ggcagtagtt cgaggtgggt aaagcccatc catttagcat
     8641 aaggcaccag ttgatcggcc agctcgcggt agctcaacca gaaattgttg tcggtgtggc
     8701 gacgccagga gcccagatga acttcataaa tagagattgg cgcatcaaac tgattcgctt
     8761 ttttgcgctc ttcagtccgt acaacctttt ccggcagccc gcaaataaga gacgcggttt
     8821 ccgggcgcat ttgcgcttcg aaggcataag ggtcggactt cagacgcaag ttgccattgg
     8881 catcaatcat ctcgtattta tagagctgac cgttatgcgc cccagggata aacagttccc
     8941 agatgccgct ctctttacgc aggcgcatcg ggtgacggcg accgtcccag tagttgaatt
     9001 gcccaaccac cgagacccga cgggcgtttg gagcccagac agagaaacgc gtacctgtga
     9061 cgccatccat agtatctgca tgcgcgccta aggtttcata cgggcgcagg tgagtacctt
     9121 cagataatag ccaggcatcc atttcctgga ttagcggacc aaaacggtaa ggatcatcaa
     9181 tcaggttttg ctgaccatgc cagacaacag ccaactgata gcggaaaaaa ttcttacgtc
     9241 gcggaatgac gccgctaaag aatccccgtg agtcgagaca ctccagtttt gcgagtttgc
     9301 gcccggtttt cggttcaatc acccacacat cggtagcgtc gggtaaaagg gcacggactt
     9361 ccagtcccgc ggtggtttta tgcattccca gtacggaaaa aggatccgca aaatggcctg
     9421 caattagcgc gttaatcacg tctctatcga tacgatcgga catgcttgtc ttcctgtttt
     9481 attgtgtcac cccatccaat tggatttttg acttctggtt gtgacatttt tttgacctga
     9541 acggcgcagc actctgtgca tcctctctgc gtcgtcctca cttcaggtaa ggctgtgaat
     9601 actcatgtat tcagccaccc ttaaagaata gccaatgctc tatttaactc ccggtaaatc
     9661 atgaaacatc tgcgcttact cctgtattac gcactaacag gggcggcatc gcgccccaga
     9721 tttaatgaat aaagattacg ccagttgacg aagcatccga cgcaacggct ccgcggcccc
     9781 ccacagcagc tggtcaccca cggtaaaggc tgacaggaac tccggtccca tattcagctt
     9841 acgcaggcgg cctaccggcg tggtcagcgt gccggtaacg gcagctgggg ttagctcacg
     9901 catagtgatt tcccgatcgt tcggaacgac tttcgcccac ggattgtgtg cagccagcag
     9961 ttcttccacg gtcggaatag acacatcttt tttcaattta atagtgaatg cctggctgtg
    10021 gcagcgcaat gccccgacac gcacacataa accatctacc ggaattacgg aagatgtgtt
    10081 gaggatcttg ttggtttccg cctgcccttt ccactcttcg cggctctgac cgttatcgag
    10141 ctgtttgtcg atccacggaa tcaggctacc cgccagcggc acgccaaagt tatccaccgg
    10201 cagctcaccg ctacgggtta aggttgtgac tttgcgttcg atatcgagaa tagcagagga
    10261 cggggtcgcg agttcatctg ccacatggcc atacagatgg cccatctggg ttaataactc
    10321 acgcatatgt cgcgcaccac cgccggaagc ggcctggtag gttgcaacgg acacccaatc
    10381 aacaagatca ttggcgaata aaccacccaa cgacatcaac atcaggctta cggtacagtt
    10441 accgccaaca aaagtcctga tgccattatt taatccatcg gtaatgacgt cctgattgac
    10501 ggggtcaaga atgatgatgg catcatcttt catgcgcaga gacgatgctg cgtcaatcca
    10561 gtaaccttgc catccgcttt cacgaagctt tggatagatt tcgttggtat aatcgccgcc
    10621 ctgacaggtc acaatgatat cgagggcctt tagcgcctcc agatcaaagg catcctgaag
    10681 tgtgccagtg gttccgccaa aagacggcgc agcctgacca agctgagaag tagaaaagaa
    10741 gacagggcga atggcgtcga agtcgcgctc ttcaaccatg cgttgcatga gaacggagcc
    10801 gaccataccg cgccagccga taaaaccaac atttttcata agcgtttttt cctgcaaaga
    10861 tgtgtgctgt ataaatgtgc cggtctcctc ttggcacatc tttcaccata caaaaagcag
    10921 ccaaagtcgc aagtgaaatt aatcaatgat agcgaagcca tcagtaatgc gacttatcct
    10981 gctttgttag cacgcagaaa gtccgcggca attatcaggg aatttgagtt atgaatgaaa
    11041 tcatttctgc agcagtttta ttgatcctga ttatggatcc gctcggaaac ctacctattt
    11101 tcatgtccgt actgaaacat actgaaccga aaagacggcg ggcaatcatg gtgcgagagt
    11161 tgcttattgc tctcctggtg atgctggtgt tcctgtttgc gggtgagaaa attctggcat
    11221 ttctcagcct gcgagcagaa accgtctcca tttctggcgg catcattctg tttctgatcg
    11281 ccattaagat gattttcccc agcgcttcag gaaatagcag cgggcttccg gcaggtgaag
    11341 agccgtttat cgtgccgttg gcaattccgt tagtcgccgg gccgactatt ctcgccacgc
    11401 tgatgttgtt gtctcatcag tacccgaatc agatgggaca tctggtgatt gctctgctgc
    11461 tggcctgggg cggcaccttt gtcatcctgc tacagtcttc gctattttta cgtctgctgg
    11521 gcgagaaagg ggtgaacgca cttgaacgcc tgatgggatt gattctggtg atgatggcaa
    11581 cccagatgtt cctcgacggc attcgaatgt ggatgaaggg gtaacgagga gagtttttcg
    11641 aaaagagccc ggtagcgtca gaactaccgg gtaaacaaac ttaactcaac agctggaacg
    11701 caatcatccc aacgatggca ccgacagtgc cgaggatggt ttccatcatg gtccaggttt
    11761 tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa caaccagaaa ccggcgtcgt
    11821 taacgtggct gacaacaatc gaaccaccgg cgatacaaat cgacagcgcc gccatttgcg
    11881 caccagagta gttcagttgt tcaatgactg gcatcaccag tcccaccgcc gttaaacagg
    11941 ctacggtggc agacccctga atgatgcgca ctgcagctgc cagcacgaag caggtgatag
    12001 caatcggcag gcccatgccg gttaacgctt cgcccagtgc cggacctacg ccagagtcaa
    12061 ccagcacctg tttgaacacg ccgcccgcac caatcaccag cagaatgatc cccgccggtt
    12121 gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg catgccctga cgcattgcca
    12181 ggccataaat cgctaccaga caagcaacca gaatcgcggt aaacggatga ccaataaact
    12241 cgaaccattc gtaagcggta gagccttctg gcacaaaacg cgcggcaatg gttttcagcc
    12301 ccaccagcac cagcggcaac aggatcagcg acaggctgaa tccgaaagat ggcattttac
    12361 cttcgccgag atgcggttcg ctgatgtcgt caggaatatg cagctcaacg taacggctga
    12421 tgaagttacc ccacagcggc ccggcaataa tcattcccgg aattgccgca cacaggccaa
    12481 tcaggatcat ccagccaaag tcggcgttca tctgcgatgc cagcagcatt ggcgctggcc
    12541 ccggcaccag gaacgcagcc gctgccgcca cgcctgcaaa taatgggatt accagcttca
    12601 ccaggttcgt accggtatga cgcgccattg agaaagcaac gctaatcagc agaacaatcg
    12661 ccacttcaaa gaacagcggt agcgcacaaa ccagccccgc aaggccaatg gcataatgcg
    12721 cgcggctgtg accgaaggat ttaagcattt tgacggcaat ctgatcgact gcgccggttt
    12781 catgtaagat cttgccaaac atagctccca gggcgacaac caccgccagg aagccgaggg
    12841 tgcctcccat ccctttttcc atcgtcgctg cgattttatc gagcggcata ccggaaaaaa
    12901 ggccagcccc catggacacc accattaaag ccaggaaagc gtgcatacgc gccttcatga
    12961 ctaaaaacag cagcagtaaa acagacccta ctgctgttaa aacaagcgtt aatgtagtca
    13021 ctacttattt gcctttttta ataacctcaa tggtgcttgc cacaacacct tccagcggtt
    13081 gatcgatatc caccaccagt acatcggttt cgtccgcacc cggctcctgc agcgtttcaa
    13141 actgcgtcac caacatttgg gttttaaaga aatggccttt gcgcgctttc aggcggcttt
    13201 caatcacatc aaaatcacct ttcaggtaga tgaaagagag attcggatta ccttcacgca
    13261 gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat cagtgacact ttattagtgc
    13321 gctgcatagc aaacgcggcg tcgttcagcg cctgcagcca cggtttgcga tcgtcatcat
    13381 tcagcggttc gccagacgcc attttttcga tattgcgccg tggatggagg aaatcgccat
    13441 caagaaacgc ggcatgaagt tgatgcgcca cttcacttgc gaccgcagat ttgccgctgc
    13501 ccgatacgcc catcaagacg taaatgtggt gatcatggtt agtcgtgctc aaagtggtgc
    13561 ccccacaata caagaattaa aaatgttacg ggtaactgtt atcggtaaca ttgtccagcc
    13621 ggacaatatc agaagcaata tccatccgtg ccttaagtgt ataagtgtga gctacttcaa
    13681 atttgtgggc ttaaatagat ccgcccggtg acaaggtgaa acctaaatct aacattttcg
    13741 gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc agcgccaata ctgcccatcc
    13801 gctcacgcgg cgtcaggacg ctcgccaacc gtggctccat cacctggcca atgtcatgac
    13861 cgtggaaacc ggcaatcgcc atatcgtcag gaactttcaa ccccagacgc tgacattcaa
    13921 acgccgcgcc gaccgccagg tcatcattag tacagaacac gccatccagc tgcggatatt
    13981 cccgccgtgc ctggcgaatc agttcaatac cggaagaata agaagaagat tgctcaacca
    14041 tcacgctata cggcaccagg cctgcatcca gcatcgcctg ttcatatccc ttctgtttga
    14101 tgatagtacg ttcgtcgaga cgtgcgccga gataggcaat gtggcgatgc ccgcgagcaa
    14161 taatggctgt ggtcatctgg cgtgccgctt caaagttatc aaaaccgacg gcgatatcga
    14221 ggcaaggcga cttgctgtcc atcagttcca ccaccgggat ccccgccact tcaatcatct
    14281 ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag tccgtcgata ttccatgaga
    14341 gcatggattc gagacgttct tgctccattt ccggtttata accgtagtgg gccagcatgg
    14401 tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg taatacttcc gcgaaaacct
    14461 ggttggtgag agaaggtaac aggacgccaa tcgcccggct ggtggcgtta gagaggatat
    14521 cgggtgcgcg attgggaata taacccagtt catcaagagc ggcggcaatc ttgccgcgta
    14581 gagcgacgga aacctgctcc gggttacgca aaaagcggct gaccgtcatt ttggtcacgc
    14641 ctacacggtc agccacatcc tgaagtacgg gtcttttctt tttcatcgtc ctgaaggtac
    14701 aaaagagata gattctctta gtttaacacg gacgcatcag aaccttcccg gggaaaacag
    14761 tgcttgttta caattattag atatacacta aatcaattgc atcgcattgt gctaacgacg
    14821 cggcaaaacc ccggaagcgt acatcagcac gcgaccggag cgagcgagga agcctgcaaa
    14881 aaggcaggct tcaaagatgc cgttgagctt taaaccggcg gcagatcgaa cagtaacact
    14941 tcgctgtcgc tatccgcatg gatggagatt gcctgctcat cccagattgc cagaccatcg
    15001 ctgatcgagg ctttcacgcc gttaatggtg acattgcctt tcaccacctg gatccagacg
    15061 cggcgttcag cggcaatctg atgcaccgac tgctcatctt tcagcaacgc ccagcggtac
    15121 agttccatat cctgatgcac tttcaatgaa ccatcacgcg catctggcga aagcaccagt
    15181 tgtttgccct gcactgcatc gaagcgacgc tgttcataac gcggcgtaat gccgttttct
    15241 tccggcatga tccagatctg atacaggtgc agacgctcgg tgctgcttgg gttgtactct
    15301 gagtgacgaa tacccgtacc cgcactcata atctggaact cgcccgccgg aacctgctct
    15361 ttattgccca tgctgtcctg gtgctcaaca gtaccttcca gcacgtaggt caaaatttcc
    15421 atatctttat gcgggtgagt gccgaagccc tgccctgctt caatcacgtc gtcgttaatc
    15481 acgcgcagcg cggagaagcc cataaagttc ggatcgtaat agttggcaaa agagaaagta
    15541 tgccaggagt ccagccagcc atgatttgca tgaccacgtt catttgcttt gcgtaagtag
    15601 atcatttgta ttcgccccct gaatgatttc gatgggctta gtgtggaccc gatctgcctg
    15661 ggatgataga gggtgaaaat tgacccctct gttcaaaaaa attgaactat tcgaggggcc
    15721 atcaaactta cttcgcgaga gtcactgtgg agggagaggc ttgctcaaat ccgcgttcaa
    15781 ggatttccag attggtaaga acttcagatt ccttgacgta atttggcgca ccgttggtga
    15841 tggtttgata caacgcatca taaacgcgcc cgtaatcgcc catctccggc ttcatctctt
    15901 ctctgaccgt cacaccctcg tcattgacat actccagcac accgaccgaa tcatccgctg
    15961 cgaatcccgg ttcgcccggc ataatattag ccttgaggct ggtttcctgc tggtcgatac
    16021 cgtatttaat aaacgaacct ttcttaccgt gaacgataaa tttcggataa tcgattttca
    16081 ccagatggct ggttttgacg atggctttca agtcgccata aaacagttgc gcttcaaagg
    16141 tgtcgtcagg attggcttta ttgcgcaggc tgcggatgtc ataagcgacg tgatccgggc
    16201 gaccgaacag agaaataatc tggtccatcg tatgcacacc aaggccatag aacgcgccat
    16261 cctgcggcaa cccaggtttg gtttctgcca ccgggcggta atagtcaaaa tggctttcca
    16321 cttcaacaat ctctcccaac ttgccgcttt caatcgcttt tttcgccgtc aggaagcagg
    16381 aatcaaagcg acgattctga tacggcgtga cggtcagccc tttgcttttc gccagcgcaa
    16441 acagctcctt cgcctgggca agcgtcgggg tgaacggttt ttcgaccagc acatttttcc
    16501 cggcttccag cgcgcgtttc gcgtattcga aatggctgtc cgcgtgggtg cagacgacaa
    16561 ccagcttaac atcgggatcg tttagcactt cgtcgagatc gctggtgaaa tggatatgag
    16621 agtaaatggg agcctgttct tccggctttg catggcggcg aaaaatatgc gcgacatgcc
    16681 agctatcctt gcggttaagt acatacggca gatggtaacg ggtggtgctt ttgccgaagc
    16741 caataaaggc gcagttgatg accatgattt cgtccttttt aaggtggtta tttacacctt
    16801 agcgcaaagc ggacgtggtt cctactggag tgcgcataac gaacacaagc acttcccgag
    16861 gataaattga gaacgaaaga tcaaaaaaaa agccagcacc cggctggcta agtaatactg
    16921 gaagcaatgt gagcaatgtc gtgctttcag gttctccgcg agggtcttcc tgatcgcgag
    16981 acaataataa tcattctcat tcgcacttgt ccaacacttt ttgcaaaaaa atgcatttga
    17041 ctcgcattcg aaagtcaatg atgttgaaag ggacatttac cccaaagagg acaaaggaat
    17101 gagtgagata gtgatacgcc acgcagaaac acgggattac gaggccatca ggcagattca
    17161 cgcccagccg gaggtgtatt gcaacacact acaggtgcct catccctccg atcatatgtg
    17221 gcaggaacga ctcgccgatc gccccggcat caagcaactc gtcgcctgta ttgatggaac
    17281 cgtagtaggt catctcacca ttgatgtaca gcaacgcccg cgccgcagtc atgttgccga
    17341 ttttggtatc tgtgtcgact cccgttggaa gaaccgcggc gtcgccagcg ccctgatgcg
    17401 agagatgatt gaaatgtgcg acaactggtt gcgggtagat cgtattgagc taacggtgtt
    17461 cgtcgataac gcgccagcaa ttaaggtcta taaaaaattt ggctttgaga ttgaagggac
    17521 tggcaagaag tacgcattac gtaatggtga atatgtcgat gcgtattata tggcgcgggt
    17581 gaagtaagat agtgcccttt ttctgagatg gaaaaagggt gtcattcaaa atcggcatac
    17641 cttcctttaa ggtatttatt ttcccaatac atatattgag atttaactca taattacttc
    17701 ctaaagtgta atattttatt ttttaatata tacgcctaca atttcctggc gtaaataaat
    17761 aacaattaac aagcataata ttgccattga taaaatagca tgccataaaa ggacttttca
    17821 gggatgagta atattgttta cctgacagta acgggagaac aacaaggaag catctccgca
    17881 ggttgtggga cttctgagtc tacaggtaat cgctggcaga gcgggcatga ggatgaaata
    17941 tttacattct cactcttaaa tagtattact aatacggggc ttggtt
//
LOCUS       DAZGAP010000109        17942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGAP010000109 DAZGAP010000000
VERSION     DAZGAP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.109"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..373)
                     /locus_tag="ACHLAM_003202"
     CDS             complement(<1..373)
                     /locus_tag="ACHLAM_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854685.1"
                     /translation="MSIDFTPCMINTYHGDIYNRTTDTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQYQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEK"
     gene            complement(954..1955)
                     /locus_tag="ACHLAM_003203"
     CDS             complement(954..1955)
                     /locus_tag="ACHLAM_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7854686.1"
                     /translation="MSSWLAMLQDATEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            2062..2358
                     /locus_tag="ACHLAM_003204"
     CDS             2062..2358
                     /locus_tag="ACHLAM_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7854687.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2481..2906)
                     /gene="arsC"
                     /locus_tag="ACHLAM_003205"
     CDS             complement(2481..2906)
                     /gene="arsC"
                     /locus_tag="ACHLAM_003205"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO7854688.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(2919..4208)
                     /gene="arsB"
                     /locus_tag="ACHLAM_003206"
     CDS             complement(2919..4208)
                     /gene="arsB"
                     /locus_tag="ACHLAM_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO7854689.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVILHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLFLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTSKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(4263..4616)
                     /gene="arsR"
                     /locus_tag="ACHLAM_003207"
     CDS             complement(4263..4616)
                     /gene="arsR"
                     /locus_tag="ACHLAM_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO7854690.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLSLLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            complement(4864..5079)
                     /locus_tag="ACHLAM_003208"
     CDS             complement(4864..5079)
                     /locus_tag="ACHLAM_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854691.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            5358..5441
                     /gene="dinQ"
                     /locus_tag="ACHLAM_003209"
     CDS             5358..5441
                     /gene="dinQ"
                     /locus_tag="ACHLAM_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO7854692.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5495..6847)
                     /gene="gorA"
                     /locus_tag="ACHLAM_003210"
     CDS             complement(5495..6847)
                     /gene="gorA"
                     /locus_tag="ACHLAM_003210"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO7854693.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6919..7761)
                     /gene="rlmJ"
                     /locus_tag="ACHLAM_003211"
     CDS             complement(6919..7761)
                     /gene="rlmJ"
                     /locus_tag="ACHLAM_003211"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO7854694.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            7964..10006
                     /gene="prlC"
                     /locus_tag="ACHLAM_003212"
     CDS             7964..10006
                     /gene="prlC"
                     /locus_tag="ACHLAM_003212"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO7854695.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILIRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            10014..10766
                     /gene="rsmJ"
                     /locus_tag="ACHLAM_003213"
     CDS             10014..10766
                     /gene="rsmJ"
                     /locus_tag="ACHLAM_003213"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO7854696.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10815..12284)
                     /gene="dtpB"
                     /locus_tag="ACHLAM_003214"
     CDS             complement(10815..12284)
                     /gene="dtpB"
                     /locus_tag="ACHLAM_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO7854697.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(12599..13033)
                     /gene="uspA"
                     /locus_tag="ACHLAM_003215"
     CDS             complement(12599..13033)
                     /gene="uspA"
                     /locus_tag="ACHLAM_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO7854698.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13424..13759
                     /gene="uspB"
                     /locus_tag="ACHLAM_003216"
     CDS             13424..13759
                     /gene="uspB"
                     /locus_tag="ACHLAM_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO7854699.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13830..15329)
                     /gene="pitA"
                     /locus_tag="ACHLAM_003217"
     CDS             complement(13830..15329)
                     /gene="pitA"
                     /locus_tag="ACHLAM_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO7854700.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15561..16754
                     /gene="yhiN"
                     /locus_tag="ACHLAM_003218"
     CDS             15561..16754
                     /gene="yhiN"
                     /locus_tag="ACHLAM_003218"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO7854701.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(16841..17893)
                     /gene="yhiM"
                     /locus_tag="ACHLAM_003219"
     CDS             complement(16841..17893)
                     /gene="yhiM"
                     /locus_tag="ACHLAM_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO7854702.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4701 a         4460 c         4570 g         4211 t
ORIGIN      
        1 ttttttcgta tctaatgtta ttaatctgat taagtattga ttgtgtctgt tgcgtaagtt
       61 gtgtcgaact tatcgtttca atgttagtaa taagacttac aagttgagcc acaacttttg
      121 agtagtctgc atttgtatat gtagtgttcg tgtggcacgc attaatttgt tgaagttggt
      181 attgcgctaa atcatattga tcagaggtat atccttctcc agagaatgtg gaattaatgg
      241 gttgttgttc ttcccaatta tcgcaaggcc acttcggtgt gtctggtgtt ttaacattgt
      301 cggtatctgt tgtgcggtta taaatatcgc cgtgatatgt atttatcata catggggtaa
      361 agtcaattga cataacattc cttattgttc atctatttcg ctaaattttt gtaaatcgga
      421 gaattagagc aggaatggat tttcttctac gcggaaataa acacaatgcg tcatatcttc
      481 ctgatataat tacctttaac ttccctttca tttccacccg gtaatatttg tgtataaaaa
      541 aagggcagtg atagtatcgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta
      601 aaaagttatg atgttcaccg atagtgtgtg aagtgatatt tttgtgatgt tttagatgtg
      661 taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg tattggcgac tgaaatctgc
      721 cctttaaaag gggggatagc actgaaacaa agagacattg cttctccgat aaaatgtcag
      781 agaagcatga ttgcgtggtg attgtcgatt gataacttac tgacggatcc gtcatcattc
      841 tcaaagcatt agttaagtta tttgtgattt ttaaggatta tcctatctat gattttctac
      901 tcagaaggat aaggcactca caatgctcct tatagtttac tgctggcatc tatttaaaat
      961 agcatttgag tcaaatagcc catcaagata gccataccta atataaccgt caggaatgca
     1021 actatcatcg gtattctgaa cattgattta agtaatatca cttccgtcag gcttgcgccg
     1081 gcactgccga tgattaatgc cattaatgct cccagaccca ttccttttgt catcagaaca
     1141 gatgccagag ggataaccgc ctctgcccgg atatatagcg gaataccgac aacggcgctc
     1201 aatgggatgg cgaagggatt atctgcccct gcatgagcgg caatccactc tgagggaata
     1261 aagccataaa taaaagagcc tattaatacc cctaacaaaa gatatggcag tacatcttta
     1321 aactgttgca acgcatcttt ggtggctagt tttatgggat taatacttac cacagctttg
     1381 gtactgcaac agtttactac aggtttctca atggctttag ccgctggact acagcaactc
     1441 acttttattg gcgtatatgt cgtccccggc gaagttttgg ctggagcaca acaatttgct
     1501 gatgagcttt tactggcaat gatatgacgt tcaaatccca gagaatcgag gataatactg
     1561 gcaagtacgg agacgccggc ggcgataatc gcatacaaca aggtaacttt ccagccaaag
     1621 gtcacccaca ttaacccgac gataatggga ttaagtaatg gggaaacaaa taaaaaagtg
     1681 agggtcggac caaacccggc tttcgctgat aacaatccac gtaacatagg gattgtcgag
     1741 caactacaga acggggtgac ggctcccaac agagcagcca ggagatagcc tttccctttt
     1801 cttgccccca tcatctgctg gattttatgg tctggcacct tttgtcttat caggctcaca
     1861 ccggcactaa tcactataaa cagcaaagaa agctcgacgg cgagaaacac aaacatctct
     1921 gtggcatctt gcagcatcgc aagccaacta ctcatacata acctcattga ataaatctat
     1981 atttccagaa taatcgaaat gaaattcaga tcaagatatt tctggtatta tcgaaatatc
     2041 aaccagacag agggggatca tatgcagttg gaagaagtcg ctaaagcgct gaaagagcta
     2101 ggtcacccga cccgtttgtt catatttaaa catcttgtca aagccgggga acaagggttg
     2161 ccggtcggtg agttgcaaaa gcagcttggg atacccggct ctacactcag tcaccatatt
     2221 tcagcactgg tatcggtagg gctggtcaaa caaaaccgtg agagtcgaac gctgatgtgt
     2281 gtttcgcaat acgcaatgct ggaagcgata attgaattct tacgcgagga gtgctgcgta
     2341 aatagcaaaa ttgcctgatg cgctacgcgc tacagatttc atgccatgca cggttttgta
     2401 ggtcggataa gacgcgttag cgtcgcatcc gacataaaca aagcgcactt taccaactat
     2461 gggcagtgga tatcgccgct ttatttcaag cgtttacccg cttcatcaac cactttctcg
     2521 ccatcttcct tggtgaacgc gccttgttgc gcatctggca gaatttccag cacgacttct
     2581 gaagggcggc acaggcgagt tcccaacggc gtcaccacaa tcgggcgatt aatcagaatc
     2641 gggtgctgaa gcataaagtc gattaaccga tcgtcagtaa atttatcttc tgcaaggccc
     2701 agttcctcat acggctcgac gtttttacgc agcagcgcgc gtacggaaat ccccatatcg
     2761 gcaatgagtt tgaccagttc atcacgcgtc ggtggggttt ccagataata gacaatggtc
     2821 ggttctgtgc cgctgttgcg gatcatctcc agcgtgttac gcgacgtgcc gcaggccggg
     2881 ttgtgataaa tggtaatgtt gctcatatca atatctcatt acaaagtgaa agagagacgt
     2941 agcgccagcg cagtcagcgt cacaaacagc acaggcaggg tcatgataat ccctgtacgg
     3001 aagtaatatc cccagctgat agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc
     3061 gtcgccaggc taccaatcgg ggtaattttc ggccccaaat cgcagccaat tacgttggca
     3121 taaaccatcg cttctttgat aacgccagat gccgtgctgc catcaatgga caacgcgcca
     3181 accagtaccg tcggcatatt gttcataata gaagagagga aggcggtgag gaatccggtg
     3241 ccgagcgtcg cggcccacag gccattatcc gccagcacgt tgagtacgcc agaaagatat
     3301 tccgttagtc cggcattgcg caggccataa accaccagat acatgccgag cgagaagatg
     3361 acaatctgcc agggcgcacc gcgtagcacc ttactcgtat taatcgcatg accgcgttta
     3421 gcgacgacaa ataatatcaa cgcgcccact gccgcaatgg cgctgaccgg aatgccgagc
     3481 ggttccagga cgaaaaatcc caccagcaga aacagtaaaa caacccagcc agttttgaag
     3541 gtggcaggat ctttaatcgc ttctgcggga gatttcagca gcgccatatc gtagttctgc
     3601 ggaatatctt tgcggaaata gagatgcaaa atcaccagcg tggcaacaat tgcggcgata
     3661 tccaccggca ccatcaccga tgcgtattcg cgaaagccga ggccaaagaa atctgcggaa
     3721 acgatattca ccaggttgga gacaataagc ggcaggctgg cggtatcggc aatgaatccg
     3781 gccgccatca cgaacgccag cgtagtgcct ttactgaacc ctaaagccag caacatggcg
     3841 atgacaatcg gtgtcaaaat aagcgccgcg ccatcattgg caaacagagc tgcaacggca
     3901 gcaccgagca ggacaatcca ggtgaacagc aagcgaccgc gtccattgcc ccagcgtgag
     3961 acgtgcagcg ccgcccattc aaaaaagccg gactcatcca gcagcaggct gatgataatg
     4021 acggcaataa acgccgccgt cgcgttccag acgatattcc acaccaccgg aatatcaccc
     4081 ggatggacca cgcccgtaac taacgccagt actgcgccga gcgttgcact ccagccgatg
     4141 cctaaacctt tcggctgcca gataaccaat acaatggtca ggacaaagat agcgcctgcc
     4201 agtaacataa tgcctcccgg ataaaacaca tgcgaaaata catatgtgtt tagctaaatt
     4261 ttttaactgc aaatgttctt actgtctgcg gaacaatttt gtcgagccag gttgcggaca
     4321 atcacctgaa ccttttcctg ttcacatcgc caggcctcat caataatttt cgccgcccat
     4381 gctggaatat gcggtgataa gcggtaatga acccacttac cttgcttgcg atccagcaat
     4441 agcccacttt cacgcagcaa tgacagatgg cgggatatct tgggctgcga ctgatcgagg
     4501 gcagtgcaga gatcgcagac gcataactct cccagttcgc taagcagtaa aacgatgccc
     4561 agacgggtct catcagcaag aattttgaac agttgaatgg gtaacagaaa tgacatattg
     4621 cgctcctgat tgttgcaggt agtgtctctc ttcgaagcgg ataagtcaaa aacatatatg
     4681 gtttaacgaa tgtgtaagtg cagaggaagg taataggtgt gaattttgag ttgggtattc
     4741 atttgaaagg aggtctgaat caggacgtaa aaaaaccgac tttgcgtcgg ttttttactt
     4801 tccagccctg agttggtggc tctggctgga gtgagaatgc ccacatctaa cggttcaata
     4861 ctattagcca gtggctaaca ttgcaagcgt tagcgatttc ttcagctggc ggagggacag
     4921 tttgctatac atgttggtga aagaggattt atatactttc atcgggtcgc tgccgtacca
     4981 ggccttgtcc gaatccggat gtaaaaaacc gactttgcgt cggtttttta ctttccagcc
     5041 ctgagttggt ggctctgact ggagtgagat agccatcatc taacgcatca atgctaatac
     5101 catactgaaa ctattgcaag gacatgctgg ttttataacc cgcaagtact gtatgattat
     5161 ccagttagct ctgagacatt ttcactctgg caatgcgcat aaacgctttc aaagtcctgg
     5221 tcagaagtac gggtggtgcc gttaactgat gctctggccg gagtgagaat gcccacatct
     5281 aacaatgggg catgcgccgt gacaggcagt ggatgagtaa gcggatgcat tctcactcca
     5341 tcgcatggag aaaacgggtg attgataaag caatcatcgt tcttggggcg ttaattgcgc
     5401 tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa cgtagctaag
     5461 ggctaagagc acactactct tagcccttta acatttaacg cattgtcacg aactcttctg
     5521 ccgctgtcgg gtgaatggca accgtgttgt cgaagtcttt tttggttgcg cccatcttca
     5581 gcgccaccgc gaagccctgc aacatttcat ccataccaaa accaatgccg tgaattccga
     5641 caatcttctc ttccggtcca acgcatacca gtttcatgcg gcacggctgg cggtgagtgg
     5701 tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga tcgtcgccgt
     5761 actgctcgcg cgcctgcggt tccgttaaac caacagtccc aatcggcgga tggctgaaga
     5821 ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta aacaggcgtt
     5881 cagagagacg gcgacccgct gcaactgcca ccggtgtcag ttccactgca cccgtgttat
     5941 cgcccaccgc gtaaatacct tcaacgttgg tattttgata tttatcgacg acgatatagc
     6001 ctttttcgtt agttttaacg ccagcggctt ccaggttgat gttgtcattg gcaggctcgc
     6061 gaccaatcgc ccaaatcagg caatccaccg tttcactgcg cccatcttcc agctccagcg
     6121 tcaggctacc atcagcattt ttcactactg ctttcgggat ggcgttggta tgcaactgcg
     6181 ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg aagctgcgca
     6241 gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta atcacgcccg
     6301 ccagttccac ggcgatgtaa cctgcgccaa caaccgccac gcgctctggc aaagcaggaa
     6361 gggcgaagaa gccatcagaa tcaataccgt attccacgcc tggaatatcc gggtggctcg
     6421 gacgaccgcc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg tttacctcca
     6481 gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta tttttaccga
     6541 gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg gcgatcaacg
     6601 tttcccagtt gaatttattg atagtggtat cgaaaccata atccggaccg tacatgtgga
     6661 tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacgcag ccgacgttca
     6721 cgcaggtgcc gcccagttct ttggcttcaa tcagcgcgca tttctgaccg tacatagccg
     6781 cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt
     6841 tagtcatgac atattgtcct tatcgttgat taccgcgatt gtagcgcgag acgtaatagg
     6901 tgccagcgat ggctgcaatt actccggcac gatccagctt gcggtggcgt gcccagtgcc
     6961 agctggaacc agtttgctgt gcagccacgg cagcacgtta ttcatctgct gttccagctt
     7021 ccacggcgga ttaatcacaa tcatgccgga agcggtcatg ccacggcgat cgctgtctgg
     7081 cagcaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca ggtcgtggat
     7141 catgcgctta atttgctggc gcagcaccac tggataccac aatgcgtaag tgccagtggc
     7201 gaaacgtttg taaccttctg ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc
     7261 atacggcggg tcgataagga ttaaaccacg gcgggaaacc ggcggcagct tggccttaag
     7321 ctgctggaaa ccgtcggctt tttcgacgcg cgcacggctg tctttctgaa attcagaacg
     7381 cagcaacggg taatcgctcg ggtgcagctc ggtcagttgc aggctgtcct gttcacgcag
     7441 tagctggcga gcaatcaacg gcgaaccggg gtaataacgc aactgcccgc tacggttgaa
     7501 gtgttttacc acattgatgt acgcctccag ctccgcaggc aaatcgtcct gctgccagat
     7561 acgggcgata ccttcgagat attcgccggt acgctcggca tgttcgctgc ttaactgata
     7621 acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct ctttcagcga
     7681 ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa
     7741 gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatc ggcccgtatt
     7801 cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc cgcgaaagcg
     7861 taacgtttct cattgaaatt cactacactt aaccccatgc tacacacatt atgtaaagcg
     7921 cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga atccgttact
     7981 gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg ttccagccgt
     8041 gactaaggcg ctgaatgact gccgcgaaaa cgtggagcgc gtagtagcgc aaggggcacc
     8101 gtacacctgg gaaaatctct gccagccgtt ggcagaagta gacgatgtgc tggggcgtat
     8161 cttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaactgc gtgaagcgta
     8221 cgaacaaacc ctgccgctgc tgtcagaata cagcacctgg gtagggcaac atgaagggct
     8281 gtataaagcg tatcgcgacc tgcgcgatgg cgatcattac gccacgctga acacggcgca
     8341 gaaaaaagcg gttgataacg cactgcgcga cttcgaactc tctggcatcg gtctgccaaa
     8401 agagaaacaa caacgctacg gtgagattgc cacccgtctt tccgagctgg gcaaccagta
     8461 cagcaacaac gtcctcgatg cgacaatggg ctggaccaaa ctcgttaccg acgaagcgga
     8521 actggcagga atgccagaaa gcgcactggc tgcggcaaaa gcccaggctg aagcgaaaga
     8581 gctggaaggt tatttgctga cgctggatat cccaagctac ttgccggtaa tgacttactg
     8641 cgacaaccag gccttgcgtg aagagatgta tcgtgcttac agcacccgcg cttccgatca
     8701 aggcccgaac gccggtaagt gggacaacag caaggtgatg gaagagatcc tcgcgctgcg
     8761 tcacgaactg gcgcaactgc tgggttttga aaactacgcc tttaaatccc ttgccactaa
     8821 aatggcagaa aatccgcagc aggtgcttga tttcttaacc gatctggcaa aacgcgcgcg
     8881 tccgcaaggt gaaaaagagc tggcgcaact gcgtgccttc gctaaagccg aatttggcgt
     8941 cgatgagttg cagccgtggg atatcgctta ctacagcgaa aaacaaaaac agcatctcta
     9001 cagcatcagt gacgaacagc tgcgtccgta cttcccggaa aacaaagcgg ttaacggctt
     9061 gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgtaaag atgttgatgt
     9121 ctggcatccg gatgtacgtt tcttcgaact gtatgacgag aacaacgaac tgcgcggcag
     9181 cttctacctc gacctgtatg cccgtgaaaa caagcgcggc ggggcgtgga tggatgactg
     9241 cgtaggccag atgcgtaaag ccgacggttc gctgcaaaaa ccggtcgcgt atctgacctg
     9301 taacttcaac cgcccggtaa atggtaaacc ggcactgttt acccatgacg aagtgatcac
     9361 cctgttccat gaattcggtc acggcctgca ccatatgctg acccgcattg aaactgctgg
     9421 tgtatctggt atcagcgggg tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga
     9481 aaactggtgc tgggagccgg aggcgctggc gtttatctcc ggtcactatg aaaccggcga
     9541 accgctgccg aaggagctgc tggataaaat gctggcggct aagaactacc aggcggcgct
     9601 gtttattctg cgccaactgg agttcggcct gtttgatttc cgcctccatg ccgagttccg
     9661 cccggatcag ggagcgaaaa tcctcgaaac tctggcagaa atcaagaaac tggttgccgt
     9721 agtaccgtct ccatcctggg gccgtttccc gcacgctttc agccatattt tcgccggtgg
     9781 ttatgccgca ggttactaca gctacctgtg ggccgacgtg ctggcggcag atgccttctc
     9841 gcgttttgag gaagagggca ttttcaaccg tgaaactggg cagtcgttcc tcgacaacat
     9901 tctgatccgt ggcggttcag aagagccgat ggagctgttc aaacgcttcc gtggtcgtga
     9961 accacagctg gatgcgatgc tggagcatta cggcattaag ggctgaacat tcagtgaaaa
    10021 tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg gcggcccgct
    10081 gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacaccg gagcacctgg
    10141 aactgcgcaa gcgtgatgag ccgaagctgg gcggcatctt tgttgatttt gttggcgggg
    10201 cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggaga ggcggtggcg aaagcggtgg
    10261 gcattaaagg cgattatttg ccggatgtgg tggacgccac cgcagggctg gggcgcgatg
    10321 cctttgtgct ggcttcggtc ggctgccgcg tgcggatgct ggagcgtaat ccggtggttg
    10381 ctgcattgct cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt
    10441 tgcaggagcg gttgcagtta attcacgctt ccagcctgac ggcgctgacc gatattactc
    10501 cgcgcccgca ggtggtctat ctcgatccga tgttcccgca taagcagaaa agcgcgttgg
    10561 tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat gccgatggat
    10621 tgctggagcc tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc ccggactacg
    10681 cgccgccgct ggcgaatgtc gccacgccaa acgcggtagt gaccaaaggg catcgctttg
    10741 atatttatgc cggaacgccg gtgtaaaagt aaagcagggt agcgttatcg ctaccctgct
    10801 ttctgcaaga ataattaatg gctttcgggc gtcgcaatca tgcgtttcag ccacggcacc
    10861 atcagcagca tcaccaccgc aacgcccagc gtaaccaggc caatcttacc aaacacgttg
    10921 gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt ctggtactgc ggtaaatgtt
    10981 gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat cccgagaata
    11041 aagcccatca aatgctgcgg caccagggca gcaatcatcg ccagacccag ggcgctaata
    11101 aacaattcac ccaggctctg gaacaagtaa accagcacga taaaccacgg cgatgtcagt
    11161 ccttgtgcat cagcaaacca cattcccgca gcggcggctg taagaaagcc cagcgagcac
    11221 ataaacatgc cgagagtaaa tttcatcggc atcgagaggt ctttgccttt gttacccaga
    11281 tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg gttaagcgcc
    11341 tggaagctga ctgggttgat ggaaaagccg agaatttcat gatgcacgtt gttgatagca
    11401 aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac cgcttcgagc
    11461 atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt gaatgcctga
    11521 cgaaagaaaa tgatggtgac gacgatggag agaacaatca gcaccagatt ggcgacttct
    11581 acgttgtgca tcagccatgc acagacgaag atcatcacca cgctgccaag taacacgtac
    11641 aacagtttgc tgaagctcat cggccggaag tcgggttcag aaccaatgtc tttcaccatt
    11701 ccgcgacagg cgatgtaaac caatagcgcg ataattaatc ccgcaccgca caggttgtag
    11761 gtgactgaat agccgaatct atcagcgatc acaggggcca gcgataacgc tatcaacgag
    11821 ccgatgttga tcgacatata gaacagggta aatgcgccat caagccgcgg atctttcggc
    11881 ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt accgacagcg
    11941 atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacaggcc ggtcatgaag
    12001 tagccaatcg ccagcacaag tgctccgagg acaatggtgc gtttggtccc cagcaggtgg
    12061 tcgccgacat agccgccaat ggaaatgagg ccatagacca gcgcagcaaa agcaccaaaa
    12121 gtgacaaaag cctgttcttg cgagaatcca agctgcttaa cgaagaaaac cgccagtacg
    12181 ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat catgaaaaat
    12241 gggcgaggtt gctgcagcat ccccatgggt gttgttgtat tcatatttat taaccttcca
    12301 ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc
    12361 agagattctt aatattgatg cggtgaatag attaatacac tattacaaca gaaaataacc
    12421 agctgatcac actaatgatg gaaaatatga tggtgttaaa tgttgtattg gcaggggatt
    12481 ttgattgcgt acgcagaccg tggccagata aggtgtttac gctgcaaggt aacaaggcgc
    12541 gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtag agggaagatt
    12601 attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg ttgatcagct
    12661 gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa accaccaaat
    12721 ccatatcgta tttcttgatt gcatcgacca gaacctggcc caggtcgccg ctgccgctca
    12781 gggtttcagt gattgggtag cctgcattag tggaaagctc ggtcagcgca tgatgtgtct
    12841 cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg gtgtataggt
    12901 cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg cgagccatag
    12961 agactgcttt ctctaccaga actttgcttt ccggggagag atcgaccgcg atgagaatgt
    13021 gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc agctagcgtt
    13081 tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta acccagccgt
    13141 aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc gtcaatccgc
    13201 cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt ggttaacctt
    13261 ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga tgttggtcac
    13321 cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc gggagcggta
    13381 gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa gcaccgtcgc
    13441 attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt tctcatcact
    13501 acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat atgttgatgg
    13561 agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg tttggtatat
    13621 ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct gtgagcgggt
    13681 gcgtcggcag tttattctga ccagcgcatt gtgtggtctg gtggtggtca gtctgattgc
    13741 attgatgatt tggcattaag cacttcagca aaaataacgc ataaaaaaag cgggccagta
    13801 aactgacccg ctcgttttgg tgcgtgcgat tacaggaact gcaaggagag ccagtacagc
    13861 ccgccggaaa gcagtaccgc agccggaagg gtaaacaccc aggccatcag aatactggtc
    13921 acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac agaagaggag
    13981 agtacgtggg tagtggaaac cggcatcccg gtataactcg ccaggccgat agacactgcc
    14041 gcagtcatct gggcagacat tccctgagcg taggtcatgc ctttcttacc gattttctca
    14101 ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa cgccagcgcg
    14161 accgccatga tgatccacac cggtgcatac tcgatggtgc taagcatgtc agacttcagt
    14221 ttcttcaaca ggcgctgatc gtcagcactc acgccaggca tcttcaccac tttgtcgata
    14281 gtgtcagaaa cgcacagcat aatgcggcgc atctggctac gttgatcaag cgacagctta
    14341 tcgtagcttt ccacatcggt agtcaacatg cctttcaggc ggttgagcgc gttaatggta
    14401 ttcgacggat ggcaatggaa ctccgcaggt tgcgttgcgc cagcttccgg agccggtact
    14461 aactgatcag caccggtcgc ttgtttcagc agcgcaggat gctgctcaaa gtaagcttcg
    14521 acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt catgttcacc
    14581 acgaagcctg ctggtgcgac accaatcaat accaacataa ccagaccaat gcctttctga
    14641 ccatcgttcg cgccatgcga aaacgccacg ccgatagcgg aaaggatcag cgcaatacgc
    14701 gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg ggtcaggtgg
    14761 atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat cagaccgcca
    14821 gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact taatactttc
    14881 gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt taaaccaatc
    14941 ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa gtaccaggta
    15001 cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag gccatgagac
    15061 gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata ggcaacactc
    15121 agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac ggcgagctga
    15181 gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc atggaaacca
    15241 ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag cccggtatgc
    15301 aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag gacatgaacg
    15361 cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact ttttttgatt
    15421 tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag gtaaatactt
    15481 ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt cacgcccgtt
    15541 ttttcttaga ggagcgcaaa gtggaaaggt ttgatgccat tattataggc gctggtgcgg
    15601 cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg ctgatcgata
    15661 atggtaaaaa accagggcgc aaaatcctca tgtctggcgg tgggcgctgc aactttacca
    15721 acctttatgt cgaaccgggc gcttatctga gccagaatcc gcatttttgt aagtctgcac
    15781 tcgcgcgttt tacccagtgg gatttcattg atctggtcaa taaacacggc atcacctggc
    15841 acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt gtcgacatgc
    15901 tggtggatga gtgcgagaag ggcaatgtga ccttcagatt gcgtagcgaa gtgctgagtg
    15961 tggcgaagga tgaaacaggc ttcacgcttg aactgaacgg catgactgtc ggttgcgaaa
    16021 agctggtcat cgcgaccggt ggactgtcaa tgccagggct gggcgcgtcg ccgtttggtt
    16081 ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggc ctggtgccat
    16141 tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg gcggtgcctt
    16201 ccgtgattac cgctgaaaac ggcatcgttt tccgtgagaa cttactcttc actcaccgcg
    16261 gcttatctgg accggcggtg ttgcaaattt ccagctactg gcaaccgggt gaatttgtca
    16321 gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag cgtaatgcac
    16381 atccgaatca gagcctgaaa aacacactgg cggttcatct accgaagcgg ttggttgaac
    16441 gtttacaaca actcgggcaa atcccggatg tttcgctaaa acagctcaac gtgcgtgacc
    16501 aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc actgaaggct
    16561 atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactctct tcacggacga
    16621 tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac gtcaccggct
    16681 ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt gcgcaggatt
    16741 tggtggcaga ataagaagca aaatgtgctc tcgataccag agggattaac ctgaataaaa
    16801 ccagataagg cgtccaacgc cttatctgaa gcctttttta ttattttttc gctgaacccg
    16861 cttctaatat agaaacgatg gaaaacagtg taaaacaaac tgcacctaag ccaaccagaa
    16921 ctcgcgaagg aataaagtaa gccatatcgg tttgcgcaat ttcagccagg aacgcagcaa
    16981 ggaacagaca aaacagacag gtaaataccg gaatcatggg tattctgttg gctaacgaac
    17041 aggtgcgtcg ccataccagc gctaacaacc acactttgga gaaaatactg tagcagatca
    17101 tccccaggca aatgagaata atgccggggg ccaggcgggc gcttgcatct gaactgatta
    17161 atacgtagat accaaaaata atggtaagtg agccaagcaa aataacccaa tagcaccaca
    17221 gccaatgttc tttcactgag aacgtattcc gcgtttgatg gacaatagtg gcgacaaggc
    17281 caataagaca agcgcaaatt gccgttaacc ccattagtac gtgacctgca acataatgtg
    17341 gtgtgatatc tgcactacgt aacagtatga ctgaccaaat gaaaccaaag agagtaagca
    17401 gaacgggaac ggcaattaag caattaccga ttgttgaaga ataggcctgc aatggcgtac
    17461 cgtcactctt actgcccgag gcgtttttgg ggatcaacag aaaatgtccg gaagatgccg
    17521 ctacggttga tacacaagcg gcaatcatgc caacgccgaa aataacatga ccggcgacaa
    17581 attcatctgc catcacatta ttgcttgcca acagtgacca tccccaaatc atagttgtag
    17641 cagaaccggc ataaccaata acgggaaata acctgttgta gaatttattc atgccgtgag
    17701 ttaactgaga tataataata aatgcggtag tgaacaatgc cagacatatc gcagaaagag
    17761 aaatcagaac atgccctgca acgaaatatt cactttgccc agaaccggtt aatacaaact
    17821 cacctaatgc gatgcatatt atacccatca gtaaaggaat taatttaaac aaccatccga
    17881 tatatatatt cattttaaac cccatatata ttttctgcta cacttcgaaa aacatatgat
    17941 tc
//
LOCUS       DAZGAP010000110        17774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGAP010000110 DAZGAP010000000
VERSION     DAZGAP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.111"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHLAM_003220"
     CDS             complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHLAM_003220"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO7854703.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMCMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHLAM_003221"
     CDS             complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHLAM_003221"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO7854704.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHLAM_003222"
     CDS             complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHLAM_003222"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4048..4050),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7854705.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4867..5748
                     /gene="yddG"
                     /locus_tag="ACHLAM_003223"
     CDS             4867..5748
                     /gene="yddG"
                     /locus_tag="ACHLAM_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO7854706.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LSFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            6021..7409
                     /gene="narU"
                     /locus_tag="ACHLAM_003224"
     CDS             6021..7409
                     /gene="narU"
                     /locus_tag="ACHLAM_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO7854707.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7504..11244
                     /gene="narZ"
                     /locus_tag="ACHLAM_003225"
     CDS             7504..11244
                     /gene="narZ"
                     /locus_tag="ACHLAM_003225"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO7854708.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELLLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVF
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKVGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDMVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLAQHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11241..12785
                     /gene="narH"
                     /locus_tag="ACHLAM_003226"
     CDS             11241..12785
                     /gene="narH"
                     /locus_tag="ACHLAM_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7854709.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLNVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12785..13480
                     /gene="narW"
                     /locus_tag="ACHLAM_003227"
     CDS             12785..13480
                     /gene="narW"
                     /locus_tag="ACHLAM_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7854710.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13477..14157
                     /gene="narI"
                     /locus_tag="ACHLAM_003228"
     CDS             13477..14157
                     /gene="narI"
                     /locus_tag="ACHLAM_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7854711.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14237..15130
                     /locus_tag="ACHLAM_003229"
     CDS             14237..15130
                     /locus_tag="ACHLAM_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO7854712.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFESPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGVAVILFHAEWAIEL"
     gene            complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHLAM_003230"
     CDS             complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHLAM_003230"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO7854713.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16246..16815
                     /locus_tag="ACHLAM_003231"
     CDS             16246..16815
                     /locus_tag="ACHLAM_003231"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO7854714.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(16969..17349)
                     /locus_tag="ACHLAM_003232"
     CDS             complement(16969..17349)
                     /locus_tag="ACHLAM_003232"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7854715.1"
                     /translation="MIIRPVKPSDVEPLLDMLLASGQFNQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCAPEVMTNDVWNLLMHWISPSHQRQGVGHLLINEIEKELTI
                     KQARLLLVETSSYVDFSAARAFYT"
BASE COUNT         4208 a         4667 c         4515 g         4384 t
ORIGIN      
        1 ggcgcttctt aaagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca
       61 ctctcttttt tcgcttccgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc
      121 gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgatccttt cacccagaat
      181 gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtgaatc
      241 agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata
      301 atcaccccgg tcaccagcag cacgaaaatc atgcacatga tcgaccagaa catcatcttc
      361 tgcccggcgt tgtacttacc aacatccgcc actttgtgct cattgccttt taatacttcg
      421 acaatgttca acagccacgg aatatccttc ttatccggga tattgtgatg tacaaaacgc
      481 acaaacataa acatcagcgc gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt
      541 cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa
      601 atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca
      661 ataaacttgg tgcgcacaat cattttcgac ttactcatga tgctcctcct cgtcatcgtc
      721 cacttcctta ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcgat
      781 aaagccagcc gctgccagcg gtttcaacgc gcctttccac aagttcaccg atgtgtcgat
      841 cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcaacacgta
      901 catgacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg
      961 cgctttcagt ttcgctacgc gctgttccgc cagctccagc atctccttct tggtgccgaa
     1021 gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg
     1081 atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt
     1141 aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcaac
     1201 aatcccgtta gcgtactgaa tgattgcacc agcagacggg cacgccttca ggcagcccgg
     1261 atcttcacag tgcatacagc catctttacg gatcagccac tccagcttgc cgttctgttc
     1321 ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta
     1381 aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg
     1441 acaggcttta cagccgatac aggtggaaac gtcgataagt ttcgcgactt ctgctttgta
     1501 atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg
     1561 cgtttccata gccatctgtt cgccccctta cgccttctcg atgtttacta agaacgcttt
     1621 atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata
     1681 acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg
     1741 ctgaccgttt acattcagcg gtttcagacg gcgcgttacc acagccaccg cgcggataaa
     1801 gccacgcttg ctggagacag tgacacgatc gccattggca atgcctttcg ccgccgccag
     1861 cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca atgcgtgctt
     1921 ggtccaggtg tggaagtgct ccgtcagacg ataggtcgta cccacatacg ggaactgctc
     1981 ttttttaccc atccgcagtg cgtcttgttc atacagacga acaaccgggt tagacaccac
     2041 gttcggatgc aacgggttag tgcccagcgg cgtttcaatt ggctcatagt gttccgggaa
     2101 cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat
     2161 aaacggcccg gttggcgtac ccggtgcggc attgccgaag tccggaatat cgttacccgt
     2221 ccacttgcta ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat
     2281 atccgccgaa gcacggttgt agagtacgcg acggttgagc ggccacgccc aggcccatcc
     2341 cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct ggttgccctg
     2401 ctcggtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag
     2461 atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat tagcgtcata
     2521 gagatcttcc agcgcatagc cgttgttctc tttcgccact tcgtcagact gcggttcgtg
     2581 cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac caccctcggc
     2641 ctggtacagc tcgcgcagat gatggtagat acccgccaga atttcgccgt cgttacgtgc
     2701 ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat
     2761 agaaccatct tcttcagcaa agcaggtcga aggcagacgg aatacttcag tctgaataga
     2821 tgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac
     2881 cagcggatcg ataaccacca tgtacttcag tttgctcagg cagctcacca ctttgttttt
     2941 gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttaccttc
     3001 atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc
     3061 gtagccccag ttgttctctt tctgcgcggc atcgccgtag aaagatttca tcaggctaac
     3121 gaaaaacttc ggatagttgc tccagtagtt cacctgatca gccagcgtcg ctttcggcgt
     3181 gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc
     3241 tggcaggctg gtagagagca ggcctaagtc agtcaggccc tgaatgttgg agtgaccacg
     3301 caatgcgttc acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc
     3361 catcgtacgg atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa
     3421 ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc
     3481 ggcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc
     3541 tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc
     3601 gttttcatcg aactgatagt tccaggacga tttatcgtat tgccgttttt cagcgtcgta
     3661 gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc tggcattggt
     3721 gtaatgctta acgtattcgg cattgatttt gttgttttcg atcaggtagc gcaaaacgcc
     3781 agacaggaac gtaatgtccg taccggaacg aataggtgcg taaatatccg ctacagaagc
     3841 ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc
     3901 catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca tcaccattac
     3961 gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg
     4021 agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ctgccagcat
     4081 cccgagggag cgggcaaatt tttgagtcag catcccggtt tcgttgctgg caccggaggc
     4141 acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat
     4201 aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca
     4261 gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt
     4321 ttcgctgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac
     4381 cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt tcgcgccatc
     4441 acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat
     4501 ctctttcgcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa
     4561 gcccaatgcc gctaccgttg ttccagccat accgcccgcg cagattttaa aaaattgtct
     4621 gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac
     4681 agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa
     4741 tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag
     4801 gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca
     4861 gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc
     4921 acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg cggcgcagct
     4981 gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg
     5041 cagatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt
     5101 ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt ggggatggtg
     5161 aactatctat ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc
     5221 aactggctga ttgtaccagg attattatta gccctggtcg gcgtctgctg ggtgttaggt
     5281 ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt
     5341 tatttcctgt cgtttattgg tgcgtttatc tgggcagcct actgcacagt aacgaataaa
     5401 tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc aagtctgtgg
     5461 gtttactatt ttctcacgcc acaaccagaa atggtattta gcacgccagt catgattaaa
     5521 ctcatctctg cggcatttac cttaggattt gcttatgctg catggaacgt cggtatatta
     5581 catggcaacg tcaccattat ggcggtaggt tcgtatttta cgcccgtcct ttcctcagcg
     5641 cttgcagccg tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg
     5701 gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggctaaaa taattgtcgg
     5761 gtctacggcc cggcagttaa tcagataaat tctgacaata ttaaaaaata aatttcaaat
     5821 accagaaagc cattttagtt acatttagta acacatatct gtctgtttaa tccacattct
     5881 gcccccgcag ttacgctttc ttatctattc ttctaatcag cacttcacct gattcatcct
     5941 taattctaat aactggctat tgcgccttac ctcctgtttt gtcttttttt cattaccaat
     6001 gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
     6061 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaagcat attgcacgaa
     6121 gaaacctctg gatatcagtc agttgcctac ttcttgcctt ctgtgtctgg atgctattta
     6181 gcgcagttac tgttaatctc aataaaatcg gttttaattt cactaccgat caactctttt
     6241 tattaaccgc attaccctcc gtttctggcg cattattgcg tgtcccctac tcctttatgg
     6301 tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt
     6361 gcgtctggct cggaattgcc gtgcagaatc cgaatactcc ttttgggata tttatcgtta
     6421 tcgctttgct atgcggtttt gcaggtgcga actttgcttc gagcatgggc aatatcagtt
     6481 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
     6541 taggtgtaag tgtgatgcag ctggttgcac cgctggtcat ttttgtcccc gtatttgcct
     6601 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     6661 catggatttg ggtgccatta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     6721 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     6781 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     6841 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     6901 ttggcccatt tatcggtgcc atcgcgcgat cggttggtgg tgctatttcc gataaattcg
     6961 gcggcgtgcg ggtgacgttg atcaacttca tttttatggc gattttcagc gccctgctgt
     7021 tccttacctt accgggcaca ggctccggta atttcatcgc attttacgcc gtatttatgg
     7081 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     7141 ttcgccagat aacaatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaaa
     7201 gagaagccat caccgaaacg gcagccgctc tgggctttat ctcagccatt ggcgcagtgg
     7261 gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtgg
     7321 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctactgacc tggctggttt
     7381 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgacacgtc ttatcaggct
     7441 acaaccgtgt cgcatccgac aaggcatcag atcccaattc attcctggag caggagttat
     7501 gtcatgagta aacttttgga tcgctttcgc tacttcaaac aaaagggcga aacctttgcc
     7561 gatggtcacg gacaggtgat gcatagcaac cgcgactggg aggacagcta tcgccagcgt
     7621 tggcagttcg acaaaatcgt gcgttccacc cacggtgtta actgtacagg ctcctgtagc
     7681 tggaaaatct acgttaaaaa tggtctggtg acctgggaaa tccaacagac cgactacccg
     7741 cgcactcgcc ctgacctgcc aaatcatgaa cctcgcggct gcccgcgtgg cgcaagttac
     7801 tcctggtatc tctacagcgc taaccgcctg aaataccctc tcattcgtaa acgactgatt
     7861 gaactgtggc gcgaagccct caagcaacac agcgatccgg tactggcgtg ggcatcgatt
     7921 atgaacgatc cgcaaaagtg cctgagctac aaacaagtac gtggacgcgg cggctttatc
     7981 cgctctaact ggcaggagct aaatcagctg attgctgccg ctaacgtctg gaccatcaaa
     8041 acctacggcc cggatcgcgt tgccggtttc tcgccgatcc cggcgatgtc gatggtttct
     8101 tacgccgccg gaacacgtta tctttcacta ttaggtggca cctgtttaag cttctacgac
     8161 tggtattgcg acctgccgcc cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg
     8221 gaatctgccg actggtataa ctccagttac atcatcgcct gggggtctaa cgtaccgcag
     8281 acacgtacgc cggacgccca tttctttacc gaagtacgct acaaaggcac taaaaccatc
     8341 gccattaccc ctgactactc tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa
     8401 caaggcactg atagtgccct ggcaatggca atgggccatg tcattttaaa agagtttcat
     8461 ctcgataatc ccagcgatta ttttattaac tactgccgcc gctacagcga catgccgatg
     8521 ctggtaatgc tggaacctcg cgacgacggc agctacgttc ccgggcgcat ggtccgcgca
     8581 tctgacctgg tggatggact gggcgaaagc aataatccgc agtggaaaac cgtggcagtt
     8641 aataccgcag gtgaactggt agtgccgaac ggttcgattg gttttcgctg gggagaaaaa
     8701 ggcaaatgga atctggaatc cattgccgcc ggtacggaaa ccgaattgtt gttaactctg
     8761 ctcggtcaac atgatgccgt tgcaggcgtg gccttcccct actttggcgg cattgaaaat
     8821 ccgcattttc gcagcgtaaa acacaatccg gtcctggtgc gccaattgcc cgttaaaaac
     8881 ctgacattag ccgatggcaa cacctgtccg gtcgtcagcg tttatgattt ggtactggcg
     8941 aattatggcc tcgatcgcgg gctggaagat gaaaacagcg cgaaagatta cgctgaaatc
     9001 aaaccgtaca ccccagcctg gggtgagcaa attaccggcg tgccgcgcca gtatattgaa
     9061 accatcgccc gtgaatttgc cgataccgcc cataaaacgc atgggcgctc gatgattatc
     9121 ctcggtgctg gcgtcaacca ttggtatcac atggacatga actaccgtgg gatgatcaac
     9181 atgctgatct tctgcggttg tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggt
     9241 caggaaaaac tgcgcccgca aaccggctgg ttgccgctgg cctttgcgct cgactggaac
     9301 cgaccaccgc gccagatgaa cagcacctcg tttttctaca atcattccag ccaatggcgc
     9361 tatgaaaaag tctttgctca ggagttactt tcaccgctcg ccgatgccag taagtacagc
     9421 ggtcatctga ttgatttcaa cgttcgtgcc gaacgtatgg gctggctacc ttctgcgccg
     9481 cagctggggc gtaacccgct cgggattaaa gctgaagccg acaaggtcgg attatccccc
     9541 acagaattta ccgcccaggc gctgaaatcg ggcgatttac gcatggcctg cgaacaacca
     9601 gatagcggca gcaatcatcc gcgtaatttg tttgtctggc gttctaacct gcttggctcc
     9661 tccggcaaag gccacgagta tatgcagaag tatctgctgg gtacggaaag tggcattcag
     9721 ggggaagaac ttggaccgac ggagggaatc caaccggaag aagttgagtg gcaaactgca
     9781 gcgattgaag gcaagctcga cctgctggtg acgcttgact tccgcatgtc cagcacctgc
     9841 ctgttctccg atatcgttct gcccaccgcc acctggtacg aaaaagacga tatgaatacc
     9901 tcggatatgc atccgtttat tcatccgctt tctgcggcgg tcgatccggc gtgggaatca
     9961 cgcagcgact gggaaatcta caaaggtatc gctaaagcct tttcgcaagt gtgcgtgggg
    10021 catctcggca aagaaaccga catggtatta caaccactgc tgcatgactc cccggcagag
    10081 ctttcacagc cgtgtgaagt gctcgactgg cgcaaaggtg aatgcgatct gattccaggc
    10141 aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc ctgctaccta tgaacgcttt
    10201 acctcactcg ggccattgat ggacaaactt ggcaacggcg gtaaagggat ttcgtggaat
    10261 acccaggatg aaatcgattt cctcggtaaa ctcaattaca ccaagcgtga tggcccggca
    10321 caggggcgtc cgctgattga taccgcgatt gacgcatcag aagtgattct ggcgctggca
    10381 ccggaaacca acggccatgt tgcagtcaaa gcgtggcaag cgctgggcga gatcaccgga
    10441 cgcgaacata cccatctggc gcagcacaaa gaggacgaga agattcgctt tcgcgatatt
    10501 caggcgcagc cgcgtaaaat tatctccagc cccacatggt ctggtctgga aagcgatcac
    10561 gtttcctata acgcgggata caccaacgtt catgagttaa ttccgtggcg cacgctgtcg
    10621 ggacgccagc aactctatca ggatcatccg tggatgcgtg cttttggtga aagtctggtg
    10681 gcttatcgcc cgcctatcga cacccgcagt gtcagtgaaa tgcgccagat accgccaaac
    10741 ggcttcccgg agaaagccct taacttcctg acaccgcacc agaaatgggg cattcactca
    10801 acctacagtg aaaacctgct aatgctgacg ctctctcgcg gtggaccgat tgtctggatc
    10861 agcgaaaccg atgcccgtga actgaccatt gtcgataacg actgggtgga agtgtttaac
    10921 gccaatggcg cgctgacggc ccgcgcggtg gtcagccagc gtgtaccacc gggcatgacc
    10981 atgatgtatc acgctcagga acgcattatg aatattccag gttcggaagt aaccggcatg
    11041 cgcggcggca ttcataactc ggtcacccgc gtttgtccga aaccaacgca tatgattggc
    11101 ggttacgcgc agctggcctg gggctttaat tactacggca ccgtcggctc gaaccgtgac
    11161 gaattcatca tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat
    11221 caggtacagg aggcgaaaaa atgaaaatac gttcgcaagt cggcatggtg cttaacctcg
    11281 ataaatgtat cggctgccat acctgttcgg tgacctgtaa aaacgtctgg accggacgcg
    11341 aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt ggttatccga
    11401 aaaactggga agatcaggaa gaatggcaag gcggctgggt gcgtgatgtg aatggcaaga
    11461 tccgcccgcg tctgggcagc aagatgggcg taataaccaa aatcttcgcc aacccggtgg
    11521 tgccgcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg
    11581 caccggaagg caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga
    11641 tggacaaagt gatctggggg ccaaactggg aagaactgct gggcggcgaa tttgaaaaac
    11701 gcgctcgcga ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca
    11761 ccttcatgat gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga
    11821 cctgcccaag cggtgctatc tacaaacgtg aagaagacgg cattgtgctg attgatcagg
    11881 ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca
    11941 actggaaaag cggcaagtca gaaaaatgta tcttctgtta cccacgaatt gagtcaggcc
    12001 aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt
    12061 acgacgccga ccgcatcgag gaagcggcga gcaccgagcg cgaagttgac ctctatgaac
    12121 gtcagtgcga agtgttcctc gatccgcacg atccctcagt gatcgaagaa gccctgaaac
    12181 aaggtattcc acaaaacgtg attgaagctg cccagcgttc gccagtctac aaaatggcga
    12241 tggactggaa actggcgtta ccgctgcacc ctgaataccg caccctgccg atggtctggt
    12301 acgttcctcc gctgtcaccg attcagtctt acgctgatgc gggcggtttg ccgaaaagcg
    12361 aaggcgtgct gcctgctatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt
    12421 tgagtgctgg cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc
    12481 actatatgcg ttcacaaacc gtggaaggcg ttaccgatac tcgtgccatc gacgaagtag
    12541 gcctgaacgt cgcccaggtc gaagagatgt atcgctacct cgccattgcc aactatgaag
    12601 accgttttgt cattccaacc agtcatcggg aaatggcggg cgatgccttt gctgaacgca
    12661 acggctgcgg ttttaccttt ggcgacggtt gccacggctc ggacagcaaa ttcaacctgt
    12721 tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg
    12781 aataatgcag atcctcaaag tgatcggcct gttgatggag tatccggacg agctgttgtg
    12841 ggaatgtaag gaggacgcgc tggcgttgat ccgccgcgac gcaccgatgc tcaccgattt
    12901 tgcactcgac ctgctcaacg cgccgctgct ggataaacag gccgaatggt gtgaagtgtt
    12961 tgaccgcggg cgcaccacgt cgctgctgct gtttgaacat gttcatgccg agtcccgcga
    13021 tcgcggccag gcaatggtgg acctgctggc ggagtatgaa aaggtcggtc tgcaactgaa
    13081 ttgtagggaa ctgcccgatt atctaccgct gtatctggag tatttaagca tacttccgga
    13141 cgatcaggcg aaagaaggat tactcaacgt tgcgccgatc ctcgccctgc ttggcggacg
    13201 cttaaaacaa cgcgaggcac cgtggtacgc gttatttgac gccctgttgc aactggcagg
    13261 aagccctcta tcaagtgaca gtgtcacaaa acagattcgc agtgaagagc gtgacgacac
    13321 ccgccaggcg ctggatgcgg tgtgggaaga agaacaggtg aagtttattg aagataacgc
    13381 cacggcgtgt gacagctcgc cgcttaatca atatcagcga cgctttagcc aggatgtcgc
    13441 gccgcagtat gtcgacatca gtgcgggagg tgggaaatga ttcagtatct gaacgtcttt
    13501 ttttacgata tctacccgta cttgtgtggc acggttttta tccttggaag ctggctgcgc
    13561 tacgattacg ggcagtatac ctggcgtgcc gcctccagcc agatgctgga taaacgcggg
    13621 atggtgctat ggtcgaactt gttccatatc ggcattctgg ggatcttctt cggtcatctg
    13681 ttcggcatgt taacgccgca ctggatgtac gcgtggtttc tgccggtggc ggtaaaacag
    13741 cagatggcga tgatcctcgg cggaatatgc ggggtattga cgctgattgg cggtgcgggg
    13801 ctactggtgc gtcgtttaac aaatccgcgt atcagggcga cgtcttctgg tgcggatatc
    13861 ctgatcctcg ccattttgtt aattcagtgc attcttgggt taaccaccat tcctttttca
    13921 gcacaacatc cggacggcag tgaaatgatg aagctggtgg gctgggcgca aagcattgtc
    13981 actttccagg gcggtgcatc tacgcatctt gatggtgtcg cctttatatt ccgcgttcat
    14041 ctggtgcttg gaatgaccat cttcctgctc ttcccgttca cccgactggt gcatgtctgg
    14101 agcgcgccgt ttgagtactt tactcgtcgg tatcagattg tgcgttcgcg gcgttaattc
    14161 tcacgcgggc cggacagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
    14221 ccacaggaga acattaatga aaccgcaggt ttaccacgtc gatgctttta cctctcaacc
    14281 gtttcgcggc aattctgccg gtgttgtttt ccccgccgat aatctcagtg aagcgcaaat
    14341 gcagcttatc gcccgcgagt taggtcattc ggaaactgct tttctgctgc acagcgacga
    14401 cagcgatgtg cgcatccgct actttacgcc aacggttgaa gttccgattt gtggtcacgc
    14461 gacagtagct gcgcactatg tacgtgcaaa ggtgttaggt ttaggaaatt gcacggtctg
    14521 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcacaacg atgattatcg
    14581 tatttcgctg gaacaaggta cgccgggctt tgagtcaccg ctggaaggtg aaacacgtgc
    14641 ggcgattatc aacgcgcttc atctcactga agacgatatt ctgccaggct tgccaattca
    14701 ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag tggatatcga
    14761 cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg gctgcaatgg
    14821 tttcttcccg ttccagatcc gcccagacaa aaacgaaacc gacggtcgta tgttctcgcc
    14881 tgccattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
    14941 gttagtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta aaggccatca
    15001 ggggcgcgca ttagggcgtg acggcgtgat tgaagtgacg gtgacaattc gtgataacca
    15061 accggagaaa gtcaccattt ctggcgtggc ggtgattttg tttcatgccg aatgggcaat
    15121 tgaactctga aaccgttgcc tgatgcgctt cgcttatcag gtctacaacc gggctaatgt
    15181 aggccggata aggcgtttac gccacatccg gcaacctgac ataaattatt ttcccgcctc
    15241 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacagtaa agccatgttt
    15301 cgcatcatct acgcccagac caaactgttc ctgcatcacg gcatataacg acgccacatc
    15361 tggtagattt cgctgctcaa ctgcgtgccc attttcataa tgggtaaaat gaaaattggt
    15421 cagcgttagc ttgccaccgt ccggcaaatg gcgacacatc agcaaatgat ggcgaaaatg
    15481 cgactgcggc cagtgcgccg accagaaatt gcccatcaca taatcgcttt gttgctgctc
    15541 gcagagatca aaacggtaca tcgactgcca gtgctgatga tgattaaact gcaacaccca
    15601 atcatcaccc tcttgcaaca accgatactc tccgtgtggc gtggtttgca tgatatccgg
    15661 cattaaacga atcggcgcgg ttagcgtctg cccaccgaaa ccgacatcag caatccattt
    15721 ttccccttcc agttccacca acagcaaacg atgggtgcgc ggcggtaatg ctggcggatt
    15781 tgataacact acgcgcccta acaagctgcg aacgttaaac cccagctcgc gtaacacccg
    15841 ctcaaacacg ccattctgct caaaacagta accaccacga cgggctatca ccagtttctc
    15901 ttccagcgat tgatcatcaa gctgtatttc cctcggcagc aaaacgtcga ggttttcaaa
    15961 cggaatggtg caattgtgtt tcaggtgcaa tgcacgaagc gtatcaatat tggccgcagc
    16021 agctcccgac cagttaatac gggcaaaata atgattcaga atgggcgtca tcaggcgttt
    16081 cccttagtgg ttttctcaac ttataacctg gaatagacgc cgcaccggct attttgtgcg
    16141 atcgcggaca tgcatcacgt tcgcaaatcg ccaatcccgt taatttcgtt acttattgcg
    16201 ttacgagcta cgcttttaaa acacccttca tagcgaggcg atatcgtgag ccaattcatt
    16261 cccgtcgaat tacaccatgc cagccgtctg ttaaatcatg gcccaactgt cttgatcacc
    16321 agtttcgatg agcaatccca gcggcgtaac attatggctg cagcctggtc aatgccggtg
    16381 gagtttgaac ctccacgcgt ggcgattgtg gtagataagt cgacatggac cagggagttg
    16441 attgagcgta acggtaaatt tggcatcgtt atcccaggcg ttgcagcgac taactggacg
    16501 tgggcagtgg gaagtgtatc tgggcgtgaa gaggataaat tttattgcta tggcattccg
    16561 gtggtgaaag gtccggtgct tggtttgcca cttgttgaag agaaatgtct ggcgtggatg
    16621 gagtgccgat tgctaccagc gacttccgcg caagagaaat acgacacgct gtttggcgaa
    16681 gtggtatcag cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat
    16741 gataagctca atacgttgca tcatttaggc gctggcacgt ttgttaccag cggcaagcga
    16801 gtcaccgcgg gctaaagaat catatgcagc aaccaagttg cttaagcaat gtttcaatat
    16861 gagttaaaag catattttat ctcacgattg catattttta gtgaagatta atttatcctc
    16921 accaacatca tagtaattgc gtattatagc ttcattgata aagccctgtt atgtataaaa
    16981 tgcgcgggcg gcactaaaat cgacatagct ggaggtctct actaaaagta gtcttgcctg
    17041 tttaattgtg agctcctttt ctatttcgtt aatcagcaga tgtcccactc cctggcgctg
    17101 atgggaagga ctgatccagt gcattaataa attccagaca tcatttgtca tcacttcagg
    17161 agcgcaatag gcaatccctg ccagtcctaa gtgttctgca ctaaaccata aatcggcaga
    17221 ttgacctgag aaataatgtg ttagtgtttt ttgtacatga tgaaggccat cttgattaaa
    17281 ttgtccactg gcaagcagca tatccagtaa tggttcaaca tctgaaggtt tgactggtcg
    17341 tataatcatg gaattcctta tctattgatg aatgtttgaa aattgtttgc gtggaacatg
    17401 gttaatctga gccaatattt ttgagaaaga atagatgggt atttatataa gcaaagaagc
    17461 catctcttta aatatcaatt aaagtataac attaacacca gacttcattg cgacaggaca
    17521 cgacaaagca cttttgcggt tttttcaggg tgcaattaca aaaattgaat cccttatgga
    17581 ctaatgtgag aaaattccgg ggcatgacta ttttgatata gtggacaatc aagcgtctcg
    17641 aattaacaat gacttttcag atgctaaacc atgcaattgg aagtatgtcg gatataggac
    17701 aacattagga aatcggacag aaaattcatc ataataaaat gacaacttat ctctttctct
    17761 cactttctga gaaa
//
LOCUS       DAZGAP010000111        17567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGAP010000111 DAZGAP010000000
VERSION     DAZGAP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.112"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..717
                     /locus_tag="ACHLAM_003233"
     CDS             <1..717
                     /locus_tag="ACHLAM_003233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854716.1"
                     /translation="YNYMVKYIEELEAAHIEELEAYLLVSGLADSRTRGLADSRTRGL
                     ISYELTPQEYNAIELLNSNNLMWGTYNLKKLFGPAIRGKRLKNADRIPGSLPFVTAGE
                     GNEGISAFISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAVVHTEFFDRNAVIFI
                     TASIHKSSCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFMETLILAVHKLVIKDV
                     VQYTDKKLVAYRQITSEKVV"
     gene            1069..1194
                     /locus_tag="ACHLAM_003234"
                     /pseudo
     CDS             1069..1194
                     /locus_tag="ACHLAM_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            1235..1310
                     /locus_tag="ACHLAM_003235"
     tRNA            1235..1310
                     /locus_tag="ACHLAM_003235"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Ile,seq:cat)
     gene            1318..1394
                     /locus_tag="ACHLAM_003236"
     tRNA            1318..1394
                     /locus_tag="ACHLAM_003236"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1352..1354,aa:Arg,seq:tcg)
     gene            1408..1484
                     /locus_tag="ACHLAM_003237"
     tRNA            1408..1484
                     /locus_tag="ACHLAM_003237"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1442..1444,aa:Arg,seq:tct)
     gene            1576..2535
                     /gene="stxA2b"
                     /locus_tag="ACHLAM_003238"
     CDS             1576..2535
                     /gene="stxA2b"
                     /locus_tag="ACHLAM_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO7854717.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            2548..2811
                     /gene="stxB2b"
                     /locus_tag="ACHLAM_003239"
     CDS             2548..2811
                     /gene="stxB2b"
                     /locus_tag="ACHLAM_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO7854718.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            3317..5227
                     /locus_tag="ACHLAM_003240"
     CDS             3317..5227
                     /locus_tag="ACHLAM_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7854719.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
     gene            5367..5549
                     /locus_tag="ACHLAM_003241"
     CDS             5367..5549
                     /locus_tag="ACHLAM_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7854720.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            5575..5847
                     /locus_tag="ACHLAM_003242"
     CDS             5575..5847
                     /locus_tag="ACHLAM_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7854721.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            5924..6139
                     /locus_tag="ACHLAM_003243"
     CDS             5924..6139
                     /locus_tag="ACHLAM_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7854722.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            6144..6677
                     /locus_tag="ACHLAM_003244"
     CDS             6144..6677
                     /locus_tag="ACHLAM_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7854723.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            6674..7141
                     /locus_tag="ACHLAM_003245"
     CDS             6674..7141
                     /locus_tag="ACHLAM_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7854724.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCLK"
     gene            7444..8250
                     /locus_tag="ACHLAM_003246"
     CDS             7444..8250
                     /locus_tag="ACHLAM_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO7854725.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            8231..9937
                     /locus_tag="ACHLAM_003247"
     CDS             8231..9937
                     /locus_tag="ACHLAM_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO7854726.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            9937..12081
                     /locus_tag="ACHLAM_003248"
     CDS             9937..12081
                     /locus_tag="ACHLAM_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO7854727.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            12239..13246
                     /locus_tag="ACHLAM_003249"
     CDS             12239..13246
                     /locus_tag="ACHLAM_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854728.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            13270..14484
                     /locus_tag="ACHLAM_003250"
     CDS             13270..14484
                     /locus_tag="ACHLAM_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO7854729.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            14540..14929
                     /locus_tag="ACHLAM_003251"
     CDS             14540..14929
                     /locus_tag="ACHLAM_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854730.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            14979..15440
                     /locus_tag="ACHLAM_003252"
     CDS             14979..15440
                     /locus_tag="ACHLAM_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854731.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQESVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            15424..15987
                     /locus_tag="ACHLAM_003253"
     CDS             15424..15987
                     /locus_tag="ACHLAM_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854732.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            15987..16637
                     /locus_tag="ACHLAM_003254"
     CDS             15987..16637
                     /locus_tag="ACHLAM_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO7854733.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            16634..>17567
                     /locus_tag="ACHLAM_003255"
     CDS             16634..>17567
                     /locus_tag="ACHLAM_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO7854734.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKASEAAEAATRAITAAGNAEESSTRAGESEKAAGADAEKARLHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGL"
BASE COUNT         4575 a         3998 c         4887 g         4107 t
ORIGIN      
        1 tataattata tggtaaagta tatagaagag ctggaagcgg cccatataga agagctggaa
       61 gcttacttgc tggttagcgg actcgcggac tcgcggactc gcggactcgc ggactcgcgg
      121 actcgcggac tgataagcta tgagcttaca ccacaggaat ataacgcgat tgagctatta
      181 aatagcaata atcttatgtg gggcacttat aacctcaaaa agttatttgg ccccgcaata
      241 agaggtaagc gtctgaaaaa cgcagatcgt atacccggct ccttgccttt tgttactgcg
      301 ggtgaaggca atgaaggtat ttctgcattt atcagtaatg atgttgttgt attccctgaa
      361 aataccatta cgatagatat gtttggctcc gcaaaataca gaaattatga gtatggtgca
      421 gatgaccatg tggcagttgt tcatacagaa ttctttgaca gaaatgccgt tattttcata
      481 acagcatcaa ttcataagtc ttcttgtacc ggaaagttca actacgggag aaatttctat
      541 gcaaaagatg ccgacgagtt aatggtatct ttgccgtcaa aagataataa ccctgattat
      601 tctttcatgg agacgctaat tctagcagta cataaactgg tcatcaaaga tgttgtccag
      661 tacactgata aaaagctggt agcgtacagg cagattacat ctgagaaggt tgtttagtgc
      721 aaatccccac ctgacagggt ggggaatggt caggttatcg tgccctgatg cagaaaaaca
      781 gtttacaatc gtaaaaatca tcatatcatg acaaaagtgg ttacattgcc acgctgctta
      841 acccgccgat gcgcgggttt tttgtatcca gaatcctgta agttatacgg aaagtacaca
      901 gaaaggaagg tgcgaccgca attaataaca aaatcttaaa aatcgcatat ggcactatta
      961 gttttctaaa tattgtatat tttaagtatt gcaggataac cctgtaacga agtttgcgta
     1021 acagcatttt gctctacgag tttgccagcc tcccccagtg gctggctttt ttatgtccgt
     1081 agcgtcaaag cagcaatggc gctggggcgt cgtgcaactg gcgttgagct ggagactgaa
     1141 cgttttgagc agacggtcag ggaagttcag gatttagcca gtcagaacgg atgatattgc
     1201 aggattagtt acgtaccgtt attatcctgc gcccggccct ttagctcagt ggtgagagcg
     1261 agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca tcacataccg
     1321 ccattagctc atcgggacag agcgccagcc ttcgaagctg gttgcgcggg gttcgagtcc
     1381 tcgatggcgg tccattatct gtacactgcg ttgttagctc agccggacag agcaattgcc
     1441 ttctaagcaa tcggtcactg gttcgaatcc agtacaacgc gccatactta ttttttctgg
     1501 ctcgcttttg cgggcctttt ttatatctgc gccgggtctg gtgctgatta cttcagccaa
     1561 aaggaatacc tgtatatgaa gtgtatattg ttaaaatggg tactgtgcct gttactgggc
     1621 ttttcttcgg tatcctattc ccgggaattt acgatagact tttcgaccca acaaagttat
     1681 gtctcttcgt taaatagtat acggacagaa atatcgaccc cacttgaaca tatatctcag
     1741 gggaccacat cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat
     1801 atacgagggc ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa
     1861 aataatttat atgtggccgg attcgttaat acggcaacaa atactttcta caggttttca
     1921 gattttgcac atatatcagt gcccggtgtg acaactgttt ccatgacaac ggacagcagt
     1981 tataccactc tgcagcgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac
     2041 tcactggttt catcatatct ggcgttaatg gagtttagtg gaaatgccat gaccagagat
     2101 gcatccagag cagttctgcg ttttgtcact gtcacagcag aagccttacg gttcaggcaa
     2161 atacagagag aatttcgtct ggcactgtct gaaactgctc ctgtttatac gatgacaccg
     2221 gaagaagtgg acctcacact gaactggggg agaatcagca atgtgcttcc ggagtttcgg
     2281 ggagagggtg gtgtcagagt ggggcgaata tcctttaata atatatcagc gatactgggc
     2341 acagtggcgg ttatactgaa ttgccatcat cagggggcgc gttccgttcg cgccgtgaat
     2401 gaagagatac aaccagaatg tcagataact ggcgacaggc cagttataag gataaacaat
     2461 acgttatggg aaagtaatac cgcagctgct tttctgaatc gcagggctca ctctttaaat
     2521 acatccggag aataacagga gttaaatatg aagaagatat ttgtagcggc tttatttgct
     2581 tttgtttctg ttaatgcaat ggcagctgat tgtgcaaaag gtaaaattga gttctctaag
     2641 tataatgaga atgatacatt cacagtaaaa gtggccggga aagagtactg gactaaccgc
     2701 tggaatctgc aaccgctact gcaaagcgca cagttaacag gaatgacggt aacaatcaaa
     2761 tcaaatacct gtgcgtcagg ttcaggattt gctgaagtgc agtttaatta atatcagaag
     2821 cattgctggt ttcgtggtgt gcagcaatgt agttacagtg taatcaatgt cacaattcag
     2881 tcagttgaag gttgtctgcc cgactgagaa tttgttaaaa aaatcctgca tggtgaatcc
     2941 ccctgagcgg aggggcatat cagcgtcaca ggtgtttctg ttttacctct atcctttctg
     3001 tgcgggttca ggtgctgata ctgaactcac cgggaggcac ccggcaccat gcatgaacgg
     3061 tacatagcgc atacaccagc ccctctccgg aggggctttc ttgtgggcaa aaaaaagccc
     3121 gagtgggttc gggcaatagc atgagatata catttttata atcgaatgga ttttaaccag
     3181 gattcataag gctgcgcaac tgcgcggcct ttttcgtatt gcgggctgta gtcttcctcc
     3241 agtcattgtc ctgtaacttc cggacttcag cccgcccctt atctgactca caacattatc
     3301 ccggccggga ggattcatgg catttaaaca ctatgacgtg gtcagggcgg cgtcgccgtc
     3361 agaccttgcg gaacgaataa ctcaaaaact gaaggaaggg tggcagcctt atggtagtgc
     3421 gctgatttcg acagctggtt atggtgcgga gttcatccag ccagttgtga gtgaggggag
     3481 catctcatca ccagaggagc caggcaaccg tccgacgacc tcagcgcctt ctgttgcgcc
     3541 agaatattac tatgtgatcg cgcttgctgg tcagtccaat ggtatgtcat acggtgaggg
     3601 actgccatta ccggatacat tcgacagccc tgatccacgt attaaacagt tagcgcgtcg
     3661 cagtacggtg acaccgggag gtgctgcatg tatctataac gatattatcc cggcagatca
     3721 ctgtctccat gatgtggagg atatgacagg gcttaatcat cccaaagcgg atttatcgaa
     3781 aggtcagtac ggctgtgtgg gacaggggct gcatatcgcc aaaaagcttc tgccatttat
     3841 accggcgaat gcgggcattc ttcttgttcc gtgctgccgt ggtggttcag ctttcaccac
     3901 cggaaccgat ggcacataca gtgacactac tggcgcctca gagagttcca cccgctgggg
     3961 ggtgggcagg ccgctgtata aggacctcat tggtcgtaca aaagccgcgc tggcgaagaa
     4021 tccgaaaaat gtgctgcttg ccgtggtatg gatgcaaggg gaatttgatt ttaacggaac
     4081 gccagcgaat catgcagccc gttttacaga agtggtggat caataccgtg cggaccttgc
     4141 agatatggcg gggcagtgtg tgggtggctc agctgacagt gttccctgga tttgtggaga
     4201 cacaacatat ttctggaagc agaagaacga aacggcgtac cagacggtgt atggtagtta
     4261 caaaaataaa acggaaaaga atatccattt cgtgccgttc atgaccgatg agaacggggt
     4321 gaatgtgccg acgaacaaac cggaagaaga cccggatatt ccggatatcg ggtattacgg
     4381 ttcaaaatgg cgaacggaca gaagcacctg gacatctcag gacagggcca gtcatttcag
     4441 ttcatgggct cgccgtggga ttatttccga ccgtctggca acggcgattc tgagctgcgc
     4501 gggtaagtct tctgcgtttg ttaatggtac tgccggggtg gttgttccag acagaccggt
     4561 taccacctca gagtctgtaa ttttttacga tgccaaaaaa gcttcagaca atcagctgaa
     4621 accttatggc tgggacggta tggatggcag acgcacactg gttgatgaca gcggcaataa
     4681 agctctgcga attgagaaaa ataacagcgc gaaatcctgg tcaatgtact gtgatattgc
     4741 tgcagacaag gcaaaacttt tactggaaaa aggcggggaa attgctgtcc ggtttaaaat
     4801 ccccgaaaac gtcaatcttg agacaaccag aaacaagtat gcctttggtt tgtactggcg
     4861 aatagcggaa tggccgggtg agggtggtga aggctatctg agttctttct ttgtccagac
     4921 agataaagcc agtattgatg ttgcatacca tcatacagtt aatcaacaaa aagaacttgg
     4981 cacgtttggc gcattcgacc atgactggca tacgcttgca tttaaattta agggcagtaa
     5041 cagcattaat gttactccgg tgcttgatgg tgtggatgga caggcgtttg acctggtgaa
     5101 atgggccaat actgctaatg gactcaacag gtttgtcatt acggatatta caggtagtgc
     5161 agaaacctac cctgtactta ttgatacggt ggaagttaaa gcaaacaaag ctggagcagc
     5221 cgcataattg ctaaaaaaag ccgccagcgg caggaatgga agctggcgga ggtaatccca
     5281 atggagaatg taaagaaaag atgctttcgt acattggttt tttaaatgaa aacagttctc
     5341 attgtcaacc ataacggtaa gaaattatga tatttattca tcaggtgatg ctgtacttct
     5401 gtacggcggt ctgtgtgctg tatcttcttt cgggtgggta cagggctgtg cgcgatttct
     5461 ggtgcaggca gattgataaa agggccgctg agaaaatcag cgccagtcag tcagccggaa
     5521 gcaaacccga agagcccgtt actccttaac aacccctttc agcgagaaaa tcccatgtca
     5581 gaaatcacat ccctggtcac tgcagaggca gtgaaggaag tcctgcgctc tgaagaagtc
     5641 cggagcgcac tgaaacagaa acttcgccag aatcttgagg cgcgtcttga tgcagaagtt
     5701 gatgccattc tggatgagct gcttggtgta caggcagagc caccgactga agcgggagat
     5761 accaccgcag agagcggtga agttcagcct gaatcaccgg tcgccgatgc gactgaacct
     5821 caacccgaat cggtcatgat gctgtaacga ggagtcaggg ccatcagtaa acggctgctg
     5881 gcctttttca tgttgtgagc ttccggattg cgggagacgg ggtatgtacc agatggaaaa
     5941 aatcacaaca ggtgtgtcat acaccacgtc agcggtggga acgggctact ggttcctgca
     6001 gttgctggac agggtttccc cgtctcagtg ggcggcaata ggcgtgctgg ggagtctgct
     6061 gtttgggctg ctgacatatc tgacgaacct gtatttcaaa attaaagaag accggcgtaa
     6121 ggcggcgcgg ggagagtaag ctgatgagca ggaaattccg ctatggttta tcagcggtcg
     6181 ttctggcgat gattgctgca ggtgcttctg cgcctgaaat cctcgaccag tttcttgacg
     6241 aaaaagaagg taatcgcacc acagcatacc gtgatggtgc gggtatctgg accatctgcc
     6301 gtggtgccac ccgggtggat ggtaagcccg tcgttcccgg catgaagctg tcgaaggaaa
     6361 aatgcgcaca ggttaatgcc attgagcgtg ataaggcgct ggcatgggtg gagaaaaaca
     6421 tcaaagtgcc actgaccgaa ccccagaaag cgggtattgc gtcattctgc ccgtataaca
     6481 ttggccccgg taagtgtctc ccgtcgacgt tttataagcg gcttaatgcc ggtgatcgta
     6541 aaggtgcctg tgagtcgatt cgctggtgga ttaaggacgg tgggcgtgat tgccgcacac
     6601 gttcaaataa ctgctacgga caggttattc gtcgtgacca ggaaagcgca ttagcttgtt
     6661 gggggataga tcagtgagaa gagtaaccgc gattatttac gctctggtta tctgcatcat
     6721 cgtctgtctg ttatgggctg ttaatcatta ccgcgataac gccatcacct acaaagcgca
     6781 gcgcgataaa aaagccagag agctggagct ggcaaacgca accattactg acatgcagca
     6841 gcgccagcgt gatgttgctg cgcttgatgc cagatactca agggaattag ccgatgcgag
     6901 agctgaaaat gaaactctgc gcgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa
     6961 cgccacctgt ccaggctccg tgcgtgaagc ccccaccacc tccggcgtgg ataatgcaac
     7021 cggcccccga ctggcagaca cagttgaacg ggattatttc accctcagag agcggttgat
     7081 gatgatgcag aagcagctgg aaggggcaca ggaatatatc cgcactcagt gcctgaaata
     7141 agttttgctg atgcgccgta tcgtcgccgt attcccgtat taacagcgac tgcagccctg
     7201 acgggtacct cctgcgtgag tgtgcgggga taatcaaaaa cgatacacac cgggtttccc
     7261 tcatttttca ggagacgtga gcatttaccc gcgaagccgc ctgtccggtg cggtggtgga
     7321 agaaaccgga taaaacaacc tcattgtaca aatatcgatc aagtatggcg ctgctgtgtg
     7381 aattctgaaa aatcacagcg gtcattatgc gtcagttttt aacagaggac gtcagaaagt
     7441 gacatggcaa agctggactg gaaaaagctg gagcaggcat tccgacgcga acatgccgaa
     7501 acgggaataa cattactgga ctggtgccgg aagaaaaaga ttaattacaa caccgccaga
     7561 acccgtataa aaatgggcaa aatcgatcat gaaattgatc ataaaaccga tcatgaaatc
     7621 gatcatgaca tctcagatga agaacccagc aatgacgcgt gttccgacga tgaaaaatgc
     7681 gcaaaaaact ctgaaaaaaa ctgcgcaaat tcggcagaaa cgaaacggat tcgtggttcc
     7741 cgacttttac ctccttcaaa cgctttttct cagcgaaaca cccacgccgt aagacaccgt
     7801 ggatatgcga agtatcttga ggcagataac ctcatggatg atgcgtccga catggtgctg
     7861 ttcgatgaac tggtgttcac ccgggcccgc gcactttcag taactaaggc acttaaaggg
     7921 atgttcgccg acctggaaga ggcaactgac gtggaaaccc gcgttgctct ttacgacaaa
     7981 atactcaaag ctgaacaggc ccttgaccgg aatattgccc gtatcgagtc aattgaacgc
     8041 tcattgctga cgctggacgt cctggctgag acagcaccaa aacttcgtgc tgaccgggaa
     8101 agaatcaacg ccgccagaga taaactcaga gcggaaaccg atattctgac cagccagcgc
     8161 cggggggtta ttacgccagt cagtgacatc gtgtcatcgc tgcatgaaat gagtaattcg
     8221 gggagactgg atgacattcc ggaagaataa aacgcgatgt gatgaacctg cagaaatgac
     8281 tgagaccgaa caacgtctgt tcattatgac aaaactgagc aatccctggt ggcgcctcaa
     8341 tcatctctac aaaatacaga acgaaaaagg tgaactggtc accttcagaa tgcgaccggc
     8401 gcaacgtcag ttgtttcgta acatgcataa taaaaacatt atcctgaaag cgcgccagct
     8461 gggattttcc acagccattg atatttatct tctcgaccag gcattattca ttccgcatct
     8521 caaatgcggg atcgtcgctc aggataaaca ggctgccagt gaaattttcc gcacaaaaat
     8581 tgctgtaccg tttgatcatc tccctgactg gctgagagcc tcattcacca tcgttgaacg
     8641 tcgtagcggt gccagcggtg gctatatcct gtttggtcac ggctcgagta ttcaggtggc
     8701 aacctcattt cgctcaggta cggtgcagcg cctgcatatc tcagagcacg gcaaaatttg
     8761 cgcgaaatat ccggctaagg cgaaagagct gcgaaccggt acgcttaatg ccgtctctga
     8821 tgaatgcatt atttttgatg aatccacggc tgaaggcgtg ggtggtgatt tttacgagat
     8881 gagtaaccgt gcacaggaga gcactgcatc aggcttattg ctgacggcac aggattataa
     8941 attccatttt tacgcatggt ggcaggatcc taaatacagc gccagagtgc ctgaaagcgg
     9001 gctgaagctg tcacgggaaa aaatgacgta tttttctgcg gttgagaagg caatgaacat
     9061 cacgcttacc gatgagcaga agcagtggta catcagtaag gaaactgaac agcgtgagga
     9121 aatgaagcag gagtttccct caacgccaca ggaggcgttt ctgacgtccg gacgacgtgt
     9181 gttcagtgcc gaaagtacat tgcaggcaga atcattctgt tcgccaccga tgattgttta
     9241 tgacattgaa cctgttacag gagcgaagac taaagctcag tctctgcgtg aaggaaataa
     9301 aaacgagttg cagcggacgc tgatgaatta tctgctggta tgggaactgc cggatccgga
     9361 tgaagagtat gtttgtgggg cagatactgc cgaagggctg gagcacggag accgctcatc
     9421 gctggatgtt gtcaaacgca gtaatggcga gcaggtggct cactggttcg ggcatctcga
     9481 tgctgaactt tttgctcatc tcatttcgca ggtctgtcgt atgtataaca acgcgtttgt
     9541 ggggccggag cgtaataatc acggacatgc agttatcctg aaactccggg aactctatcc
     9601 gacacgttat atctacaacg aacagcatct tgaccaggca tatgacgacg atacgccccg
     9661 ccttggctgg ctgacaaccc gtcagagcaa acctgttctg accgaaggaa tgaaaacgct
     9721 cctgaataat ggaatatcag ggatccgctg gtcaggcaca ttatcggaaa tgaacaccta
     9781 cgtttatgac gcgaaaggct ccatgaatgc acaggaaggc tgctttgatg atcagctcat
     9841 gagctacatg attgcccagg agatgcgcgc cagaatgccg gtgagggtaa aacagaaaac
     9901 ggataaacgc agaaccacac actggatggc acactgatga aaaatgaaat taacaccaca
     9961 gcgatgaaaa acgatcatgg atccacgccg cgtttttctc agcgtcagtt actgtctctc
    10021 tgttctgata ttgacagtca gcctctctgg cgtgatgccg caaacaaggc ctgtgcgtat
    10081 tatgatggcg accagctggc accggaagtt atccaggtac tgaaagatcg cggtcagccc
    10141 atgaccatcc ataacctcat tgcccccacg gtagatggtg tactgggaat ggaggcaaaa
    10201 acaagaacgg acctgatagt gatgtcagac gatccgaacg atgaaacaga gaaactggca
    10261 gaggccatta atgcggagtt tgctgatgcg tgccgtcttg gcaacatgaa taaggcccgc
    10321 tccgatgcct atgcggaaca aatcaaggcg gggctcagtt gggtggaggt caggcgcaac
    10381 agcgatccgt tcggacctga attcagggta tccacagtca gcagaaatga ggttttctgg
    10441 gactggctca gccgggaatc cgacctgagt gactgccggt ggctgatgcg tcgccgctgg
    10501 atggataccg atgaggcaaa agccacgttc ccgggaatgg ctcaggttat cgattatgct
    10561 attgatgact ggcgtggttt tgtcgatacc acgattactg aaggccagcc cagtccgttg
    10621 atgagtgcat gggaagagta tcagtcatgg gatcgcaggg agaatgaatg gcttcagcgt
    10681 gaacgccgtc gtgtgctgct tcaggtggtt tattaccgta cattcgaacg tcttccggtg
    10741 attgaactca gtaatggacg ggtggtggct tttgataaaa ataatctgat gcaggcggtg
    10801 gctgtggcat ccgggcgggt tcaggtgaaa gtcgggcggg taagtcgtat tcgtgaagcc
    10861 tggtttgtgg gcccacactt tattgtggat cgcccctgta gtgctccgca ggggatgttt
    10921 ccgctggttc ctttctgggg ataccgaaag gataaaaccg gggagccata cgggttaatt
    10981 tcccgcgcca ttccggcaca ggatgaggtg aattttcgtc gtatcaagct gacctggctg
    11041 cttcaggcca aacgcgtgat tatggacgag gatgccaccc agttgtcaga cagcgacctg
    11101 atggagcaga tcgaacgtcc ggatggcatt attaaactga atccggcccg aaaaaatcag
    11161 aaaagtgtcg cagatgtttt tcgggttgag caggattttc aggttgccag ccagcagttt
    11221 caggtcatgc aggaatcgga aaaacttatc caggatacca tgggagtgta ttccgcattt
    11281 ctcgggcagg attcaggtgc gacgtcaggc gtggctatca gtaacctggt ggagcagggg
    11341 gccacaactc ttgcggaaat caacgataac taccagtttg cctgccagca ggtgggaaga
    11401 ctgttgctgg cttatcttct cgatgacctg aaaaaacgcc gtaatcatgc agtggtgatt
    11461 aatcgcgatg atcgccagcg tcgccagacc attgtcctca atgctgaagg tgataatggt
    11521 gaactgacca atgatatttc aaggttaaat acacatattg cgctggcgcc tgttcagcag
    11581 acaccggcgt ttaaggcaca gcttgcacag agaatgtcag aggttattca ggggctgccg
    11641 cctcaggtgc aggctgttgt gctcgacctg tgggttaatc ttctggatgt gccgcagaaa
    11701 caggagtttg ttgagcgtat tcgtgctgcg ctggggacgc caaaatcacc ggatgaaatg
    11761 acgccggaag aacaggaagt agcggcacaa caacaggcac ttcagcaaca acaggcagaa
    11821 ctccagatgc gcgagatggc tggcagagtg gcaaaactgg aagctgacgc cgccagggca
    11881 catgcagctg cacaacggga taatgccagt gcacagcggg aagtcgccct gacacagggg
    11941 cagcgttatg tggatgcgct taaccaggca catacggcag aaatcattac cggcgtacag
    12001 aatatggaac aggagcagga cgttcttcag caacagatgc tgtatacgtt acaacagcgg
    12061 atgaatgaaa tgtcgctctg aaaactctgg cttcaactga accccgtcat cgtacggggt
    12121 tttttgtttc cggaggtaag cgttccggga gcggtgcgct tattcgcggg ggcagcgata
    12181 agccttattt actcaaccat tcggatctgt ccgataaaca gaccatgcgg agttatttat
    12241 ggattttgaa tttacgggtg aagaaacccc ggaacaactg gaaaaaatgc tggaaggact
    12301 tggggatgtg gatattgaca gtcacgcaca ggacgtcgtg acggaagata ccacggaaaa
    12361 acatgcggat gaggaagcac agactcagac gggcgataac aatgtggcac cgacgccgga
    12421 tgccagtgtg gagcagacgc aggacgtgaa ggagccggaa gcgaaggggg tgctcacccg
    12481 cgacggtaaa cacgtcattc cctatgaagt ccttgaggct gaacgttccg gtaagcaacg
    12541 ggccgaacag gaagccgcac ttcttcgtgg gcagatagct gaagaaaaac gcagggtgga
    12601 actgctgacg tctcagatcc accaggccgg tatgaagccc acaccgttac cggaaaacga
    12661 aaaaatttct gatgagcaga ttgcccgtat cagggagatg tatccggaaa ttggtgacgc
    12721 ggtggcttcg ctcatccgta aaaataacta tctccagtcc cgtgttcagc aatcaacaca
    12781 gcaggcagaa ggtaatggtg gtgaggattt atcaccggtt cttgatgcga tgaatgccgt
    12841 gccggtgctg aaaacgtggc aggagtccga tccggatcgc ttctcggttg ctgtatccat
    12901 cgacgggaag ctccagaatg accccgcatg gaaagacaaa acgctcactg aacgtttcgc
    12961 tgaagtggcc cgtcgtacgc aggttgcttt cggtgaagtc agtgagtcgt ctgctgacaa
    13021 ccaggcagac aaaacggata tccggaaaac ggcggaagaa aaagtgaaga ccgctgaaca
    13081 ggagcaggca gtacctgctt ccccgtcaga tttaggcacc acggcctccg tcggaaccgg
    13141 tgataatttt gaacggttac ttggtgcttc tcattcagag gctgaggcga ttatgcgtgg
    13201 tatgacgaat gctgaaatag acgcacttct ggagaagctc gggtaactta ctgaaggagt
    13261 actgaagtaa tgacgactgt aacatcagcc caggcgaata agctgtatca ggtggcgctt
    13321 tttactgctg ccaaccgcaa ccgctcgatg gttaatatcc ttactgaaca gcaggaagcg
    13381 ccaaaagcgg tttcgccgga caagaaaagc acgaagcaga ccagcgcagg tgcgccggtt
    13441 gtccgtatca cagaccttaa caaacaggcc ggtgatgaag tgaccttcag catcatgcac
    13501 aaactctcaa aacgtccgac gatgggagat gagcgtgttg aaggtcgtgg tgaggatctc
    13561 agccatgctg acttctccct gaaaatcaat cagggacgtc acctggtgga tgcaggcgga
    13621 cgtatgagtc agcagcgcac gaagtttaac ctggcatcct ctgccagaac gcttctgggg
    13681 acgtacttta atgacctgca ggaccagtgt gcgatagtgc atcttgccgg agcacgtggt
    13741 gattttgttg ctgacgacac tattctgccg acagcggagc accctgaatt caaaaaaatc
    13801 atgatcaacg atgtactgcc tccgacacat gaccgtcact tttttggcgg tgatgcgaca
    13861 agctttgagc agattgaagc ggcagatatt ttttctattg gcctggtgga caatctctcc
    13921 ctgttcattg acgaaatggc gcatccgtta cagccggttc gtctgtccgg tgatgaactt
    13981 cacggagaag atccatatta cgtcctgtac gtcacgccgc gtcagtggaa tgactggtac
    14041 acctcgacgt ccggtaagga ctggaaccag atgatggttc gtgccgtgaa ccgtgcaaaa
    14101 ggttttaatc atccgctgtt caaaggtgaa tgtgcgatgt ggcgcaatat cctggttcgt
    14161 aagtatgcgg gtatgccgat ccgtttctat caggggtcaa aggttctggt atcagagaat
    14221 aacctgacgg caaccacgaa agaggtcgct gctgcaacca atattgaccg cgccatgtta
    14281 ctgggggctc aggcgctggc aaatgcttac ggtcagaagg cgggcggtca cttcaacatg
    14341 gttgagaaga aaacggatat ggataaccgt actgagatag caatcagctg gatcaacggt
    14401 ctgaaaaaaa tccgtttccc cgagaagagc ggcaagatgc aggatcacgg cgtgattgcc
    14461 gttgatacag cagtgaagct ctgatttttt cctttcccca tgccgggttt tcgcccggct
    14521 ttttcaggag tcattaatta tggcaaagac tatccttgcc ccgtcactga gtgaacgggt
    14581 ctatacgggt acgcacggta atgagtcggt ggcagaaggc gtatttacgg tgaatgctgc
    14641 ggaagcggac agtgttattc atcttctctc actgccagtg ggcatccgta tcaactcact
    14701 ccagctggtt tcaacgggtg gtctgggtac tgcaaccgtc agcattaagt ccggtgagca
    14761 tgctctcatc gataacagcg aagctgtttc tgcaaaattt gccagatatg tgccagtgga
    14821 gccgtacacc acacagcgtg acggggagct ggttactgtc accattaaga ctgccgctgc
    14881 aaccggcacc ctgaatgttc tgctgcgtta taccgtggtg ggatactgat taaaaccttc
    14941 cggcccgcgt catgcgggct ttttatccgg ggaattatat gagtgagaaa attgccgttg
    15001 tctatatcgg cccaaaaccc gtgaaaaagg acaccattac cggaagccgc acattgttcc
    15061 cacgtcttga gccggtgcat gttgacagtg cgatggcctg gcaactgctg gggtttccgg
    15121 atgtctgggt tcgtcatgaa gagcttgatg atgttctgaa aaagcaacaa cagaatgagc
    15181 agttgcggca ggcacagcag gcgcaggaaa gcgtgcttgc tgcgcgggca gaagcggaga
    15241 acagttttgt tgtttctgtt aacgggcagg aggtggattt aagtaagctc acctcagcac
    15301 ggctggcgac gctgtgtgag gcagaagagc tggatattca caaagacccg aaagaaacgg
    15361 ctgaggcatt ccgtatccgg gtgcgtgagg catttcgccg tcgtgttgcg gagactgaac
    15421 agcatggcgg aactgagtga ttttttaccg tatgtccgtc gtcatatcag cggtccactg
    15481 aacattatga tgacggatgc tctgtcaatg gctgccgtgg cattcagccg ccagtcgttg
    15541 gtgtgccgtc gggaggttac tgttgtaccg gtggcaggaa aagaaatcgt gcttccgtat
    15601 gacaaagatg atgaggagtg cgttcatatc atccgtatct ctgacgataa tcatgagctt
    15661 tttgtcggtc gggatgtgga tatcagctcc ggacgctccc tgcgatttgc ctgttctccc
    15721 ggtgaggtga gcgtgcttta tgccgtcgct ccgaaagccg gacgcagcca gataccggat
    15781 gaactcctca catggcctga agaagtggct gcgggggcac ttgagcggtt gttcatgcag
    15841 actggtgttt catggtcaga tccgttacgc gcacagtatt tttctgtgca gttttctgag
    15901 gggatccgtc gggcatatcg tcatacactg gcgacaagcc cgtactcttc ataccgcaac
    15961 cctgtacgca ggcagaggtt ttactgatga cgacgattac agaaatcatc ggacgtgtga
    16021 atacacaact ggttgacccg atgatggttc gctggcccct gcaggaattg tgcgattatt
    16081 acaatgatgc tgtgagggca gtgattctgg cgagaccgga tgctggcgca agcctggaaa
    16141 caatcagttg tgttcctggc gcccgtcagg ttttgcccga tggtgtaata caacttcttg
    16201 acgtgatatg cctcagtgat ggtagtgcag tcagaccatt atcccgggag gtgctggatg
    16261 cgcagtatcc tgagtggccc acaatgaagg gcattcctga atgttttatc agcaacgacc
    16321 tgtccccgcg cgtattctgg ctgtttcctg tccctgacaa agagataagt attgatgcag
    16381 tggtaagccg gataccggag gcagtgtatg ttctgacgca ggacgatgat acgccagttc
    16441 cactggaaga ggcttatgtt aacccactgg tggactggat gttgtttcgt gctttcagta
    16501 aggatgctgc cggtggtgca gaatcggggc tggctgcgca gcattatcag agttttgttg
    16561 agcaacttgg gatcaaacag ggggcagaca gtgcattgtc tgcccgtaaa aaagtgttta
    16621 acggaggtgg agtgtgagtg ttgttgtttc ggggacgctg aaatctcctg atggtgaggc
    16681 gatatcagga gcaaatatta ccctgacggc gctgacagtt tcaccggatg cgctcagcgg
    16741 caccagtgcg tcggcagtga cccgtgaagg tggatattac ggaatgacga tggatccggg
    16801 ggagtatgcg gtttcggtga cggtgaaagg gaagactgct gtctacggac gtgtgcgtat
    16861 tgaggggacc gaaagtacgg tgacgctcaa tatgctgtta cgccgcagtc ttgttgaggt
    16921 gagcataccc ggagagctgc tgacagattt ccggcagata cagaataatg tggctgatga
    16981 ccttgctact attcgtcgcc tgaatgaaga cacggcgaca aaaaacactc aggccacaca
    17041 gtcaaaagaa agtgcagcag ccagtgcgaa gagtgcatct gacagtgcaa agacggcaac
    17101 cagcagggcg gctgaagccg gacaaaaagc cagtgaagct gcagaagcag cgacccgtgc
    17161 aatcacagca gcggggaatg cagaggaaag ctcgacccgt gccggagagt ctgaaaaagc
    17221 cgccggagct gatgcagaaa aagccagact gcatgctgaa aaggccaggc tggcgcagga
    17281 gagcgccgga gagatcctta agcgggcaga ggctgccact gtcagtgctg aagaggccag
    17341 acgtatggct gagaatgcac gggggccccg ggggcctcag ggagaaactg gtccgaaggg
    17401 ggatgtcggt cctaaaggcg aaacaggtcc agtgggccct caagggcccg cagggccgaa
    17461 aggtgagcgt ggtgacgttg gtgctcaggg ggcagtaggg cccgctggtc cgcggggtga
    17521 gaagggcgaa cagggggagc gaggaccgca gggaatacca ggcctga
//
LOCUS       DAZGAP010000112        17437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGAP010000112 DAZGAP010000000
VERSION     DAZGAP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.113"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            124..642
                     /locus_tag="ACHLAM_003256"
     CDS             124..642
                     /locus_tag="ACHLAM_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7854735.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            658..1197
                     /locus_tag="ACHLAM_003257"
     CDS             658..1197
                     /locus_tag="ACHLAM_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO7854736.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1290..2867)
                     /gene="guaA"
                     /locus_tag="ACHLAM_003258"
     CDS             complement(1290..2867)
                     /gene="guaA"
                     /locus_tag="ACHLAM_003258"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO7854737.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2936..4402)
                     /gene="guaB"
                     /locus_tag="ACHLAM_003259"
     CDS             complement(2936..4402)
                     /gene="guaB"
                     /locus_tag="ACHLAM_003259"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO7854738.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4564..5940
                     /gene="xseA"
                     /locus_tag="ACHLAM_003260"
     CDS             4564..5940
                     /gene="xseA"
                     /locus_tag="ACHLAM_003260"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO7854739.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(6009..14060)
                     /locus_tag="ACHLAM_003261"
     CDS             complement(6009..14060)
                     /locus_tag="ACHLAM_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFO7854740.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDYTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14181..15158)
                     /locus_tag="ACHLAM_003262"
     CDS             complement(14181..15158)
                     /locus_tag="ACHLAM_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO7854741.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15213..17390)
                     /gene="sinH"
                     /locus_tag="ACHLAM_003263"
     CDS             complement(15213..17390)
                     /gene="sinH"
                     /locus_tag="ACHLAM_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO7854742.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4107 a         4626 c         4312 g         4392 t
ORIGIN      
        1 tataaattat ttcccgcatg aattttaata aatttaatct attcctttat acgcaataca
       61 tttactttcc tcttttgatg atcttaaatg tcttattttt cgtaatgtgt ataacaagga
      121 atagtgatga aatttaaaaa atgtcttctg cctgtggcaa tgttagcgtc attcactctg
      181 gcaggatgcc agtcaaatgc tgatgatcat gccgccgatg tttatcaaac cgatcaactg
      241 aataccaaac aagaaactaa aaccgttaat attatttcca ttcttcccgc aaaagttgcc
      301 gtagacaacg cccaaaataa acggaacgca caagccttcg gcgcgcttat tggcgctgtc
      361 gctggcggtg ttatcggcca caacgtcggg tctggcagca attccggaac gacggcaggt
      421 gcagttggcg gcggagctgt aggcgcggca gcgggttcta tggtgaatga taaaacctta
      481 gtggaaggtg tttctttaac atataaggaa ggcaccaaag tgtatacctc cacccaggtg
      541 ggtaaagagt gccagtttac gacaggttta gccgttgtta ttaccacgac gtataacgaa
      601 acacgtattc agccaaatac caaatgtcct gaaaagagct aataatcagg aggagtcatg
      661 aagaaagttt ttctttgcgc catcttagcc tccttaagct atccggctat cgcctcatca
      721 ttgcaggatc aactctcggc tgtcgcagaa gcggaacagc aaggtaaaaa tgaagagcaa
      781 aggcagcatg acgaatgggt cgcggagcgc aacagggaaa tccagcaaga gaagcaacgt
      841 cgcgcaaacg cccaggccgc ggctaataaa agagcggcaa cggcagcggc gaataagaaa
      901 gctcgtcagg ataaactgga cgccgaagcc actgcggaca aaaaacgcga tcaaagttat
      961 gaagatgagc tacgcagctt agagattcag aaacaaaaac tggcgctggc gaaagaagaa
     1021 gcccgcgtta agcgagaaaa cgaatttatc gatcaggaac tgaagcacaa agctgcgcaa
     1081 accgatgtgg tgcaatctga agctgacgca aacaggaata tgactgaagg cggtcgcgat
     1141 ctgatgaaaa gcgtgggcaa agcagaagag aataaatcag atagctggtt taactaaacg
     1201 atgttagtaa cttcaaacct ataattcttt aggataaaaa accctctgta gtaacagagg
     1261 gttttgttca ttcatagtgc agggtcaaat cattcccact caatagttgc tggtggcttg
     1321 ccgctgatgt catacaccac gcgggaaata ccattcactt cattgataat gcggttggaa
     1381 acgcgaccga ggaaatcgta cggcagatgt gcccagtgtg cggtcataaa gtcgatggtt
     1441 tcgacagcac gcagagagac aacccagtca tacttacgac catcgcccat tacgccaacg
     1501 gaacgtactg gcaggaagac ggtgaatgcc tggctgactt tgtcgtacag gtccgcttta
     1561 cgcagttctt caatgaagat ggcgtcagca cggcgcagca ggtcacaata ctctttcttc
     1621 acttctccca gaacacgaac gccaaggcct ggtcccggga acgggtgacg gtacaacatg
     1681 tcatacggta ggcccagctc cagaccaatc ttacgcactt cgtctttgaa cagctctttc
     1741 agcggttcaa ccaagcccat cttcatctct ttcggcaggc cacccacgtt gtggtgagat
     1801 ttgatgacgt gtgctttacc ggttgcagaa gcagcagatt cgataacgtc agggtagata
     1861 gtgccctgcg ccagccactt cacgtcttcc agtttcagcg cttcttcatc gaatacttca
     1921 acgaaaacgc gcccgatgat tttacgtttt gcttccggat cattttcgcc agccagcgct
     1981 gacaagaagc gatcttctgc cggtacgtga acaatattca gaccaaagtg atcgccaaac
     2041 atatccagaa cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac gaatacgcaa
     2101 gtcaggtttt taccgatagc gcggtgcaac agcattgcgg ttacggagga atccacacca
     2161 ccagagaggc cgaggatgac tttatcgtcg cctacctgct cgcggatgcg agctacagca
     2221 tcgtcgataa ttttcgctgg cgtccacagg gcttcacact ggcagatatc acgcacaaaa
     2281 cgctccagca tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa ctgtacgcca
     2341 tagaagcgtt tttcttcgtt agccataatg gcaaacgggc agctttcggt gctggctacg
     2401 gtgacgaagt cggacgggat agcggtaact ttatcgccgt ggctcatcca gacatcgagc
     2461 agcggtttac cgtctgcggt cagagcatct tcaataccgc gaaccagtgc gctgtcgttt
     2521 acgacttcaa cctgcgcgta gccaaattca cgttcgttag aggcttcaac gtgaccgccc
     2581 aactgcattg ccatggtctg catgccatag caaacgccga ataccggtac gcctgcttca
     2641 aaaacatact gcggcgcacg cgggctgttt tcttcggtgg tgctttccgg gccgccggaa
     2701 agaataatgc cgcttgggtt gaagtcacga atttgtgctt ctgtcacatc ccacgcccac
     2761 agttcgcagt aaacacccag ttcacgcacg cggcgcgcaa ccagttgagt gtactgggaa
     2821 ccgaagtcca gaatgaggat gcgatgctta tgaatgtttt ccgtcattga cgcttattcc
     2881 gaggcaagtg aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa gagaatcagg
     2941 agcccagacg gtagttcggg gactctttag taatggtcac gtcgtgaacg tggctttcct
     3001 gaatgcccgc accgctgata cgtacaaact ccgctttagt acgcagttcg tcgatagtac
     3061 cacagccggt cagccccata caggagcgca ggccgcccat ctgctggtga atgatctctt
     3121 tcaggcgacc tttataggcc acgcgacctt cgataccttc cggcaccagt ttgtcggcag
     3181 cgttgtcgct ctggaagtaa cggtcagaag aacctttgga catcgcgccc agggaaccca
     3241 taccacggta agatttgtaa gaacggccct ggtagagttc gatttcaccc ggagattctt
     3301 cagtacctgc cagcatggaa cccaccatca ccgcgcttgc gcctgcggcg atagctttgg
     3361 cgatatcgcc tgagaaacgg ataccgccgt cagcgataac cggaatgcca gtaccttcca
     3421 gggcttctac tgcatcagca acggcggtga tctgcggaac accgacacca gtcacgatac
     3481 gcgtcgtaca gatagaacca gggccgatac ccactttaac ggcactgcaa ccggcttctg
     3541 ccagcgcgcg ggcacctgca gctgttgcca cgttgccgcc gatgatttgc aggtcaggat
     3601 atttagcgcg agtttcacgg atacgctgca gaacaccttc agagtgaccg tgagaggagt
     3661 cgatcagcag aacgtcaacg cctgcggcaa ccagcgcatc aacacgctct tcgttaccag
     3721 cgccagcacc aaccgctgca ccaacacgca gacggccttg ctcgtcttta caggcgttcg
     3781 gtttacgttc tgctttctgg aaatctttca cggtgatcat gccgatcagg tggaattcgt
     3841 catcaaccac cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc accacttcac
     3901 gggcttcgcc ttcacgcacg gttaccagac gctcttttgg cgtcatgtaa acgctaaccg
     3961 gttgattcag atcggtcaca aaacgcacgt cacgaccggt gatgatgccg accagttcgt
     4021 tttcttcggt aacgaccggg tagcccgcaa agccgttacg ctcggtcagt tctttcactt
     4081 cgcgcagcgt cgtggttggc agaacagtct gcgggtcagt caccacacca gattcgtgtt
     4141 ttttcacacg gcgaacttct tctgcctggc gttcaatgga catgtttttg tggataaagc
     4201 cgataccgcc ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg gtatccattg
     4261 ctgcggaaag catagggata ttcagacgaa tagttttcgt cagctgggtg ctgaggtcag
     4321 cagtattcgg cagaacggta gaatgtgcag gaacgaggag aacgtcgtca aacgtcagag
     4381 cttctttagc gatacgtagc atgggcaata tctcgaccag agtggttaat aaatattgcc
     4441 gcggcattat acagagcgta accgattgca tctacccctt tttgcaaaaa atgcttgcta
     4501 tccccggagg gcgggttact atcgactgaa taacctgctg atttagaatt tgatctcgct
     4561 cacatgttac cttctcaatc ccctgcaatt tttaccgtta gccgcctgaa tcaaacggtt
     4621 cgtctgctgc ttgagcatga gatggggcag gtgtggatca gcggcgaaat ctctaatttc
     4681 acacaaccgg cttccggtca ctggtacttt acgcttaaag acgataacgc ccaggtgcgc
     4741 tgcgcgatgt tccgcaacag caatcgccgg gtcaccttcc gcccgcagca tgggcaacag
     4801 gttttagttc gcgccaacat tacgctgtat gaaccgcgcg gcgattatca aattattgtc
     4861 gaaagtatgc agcccgccgg tgaaggttta ctgcaacaga agtacgaaca gcttaaagcg
     4921 aagttgcagg ctgaaggttt gttcgatcag caacttaaaa aaccactccc ctcccctgcg
     4981 cactgcgttg gggtgattac ctcaaaaaca ggtgccgcac tgcacgatat tttgcatgtg
     5041 ttgaaacgcc gcgatccgtc tctgccagtg ataatctatc ccactgccgt tcagggcgat
     5101 gacgcaccag ggcaaatcgt tcgcgccatt gagctggcga atcttcgcaa cgagtgcgac
     5161 gtattaatcg tcgggcgtgg cggtggctcg ctggaagatt tatggagttt taatgatgag
     5221 cgtgtggcgc gggcgatttt tgccagtcgc attccgatag tcagcgccgt cgggcatgaa
     5281 accgatgtga caattgctga ctttgttgct gatctacgtg cgccaacgcc gtcagccgcc
     5341 gctgaagtgg tgagccgaaa tcagcaagaa ttgctacgtc aggtgcaatc gacccatcaa
     5401 cggctggaaa tggcgatgga ttattatctc gccaaccgca cgcgtcgttt tacgcagatt
     5461 catcatcgct tgcagcaaca gcatccgcag ctccggctgg cacgtcagca aactatgctc
     5521 gaacgtctgc aaaagcggat gagctttgcg ctggaaaatc agcttaagcg tgccgggcaa
     5581 cagcagcagc gattaacaca gaggctaaac cagcaaaatc cgcagccgcg tattcaccgc
     5641 gcacagacgc gaattcaaca actggaatat cgtttagcag aaaccctgcg cgcacagctt
     5701 agcgccacgc gtgaacgttt cggtaatgca gtaacacacc tcgaagccgt aagcccactg
     5761 tcaacgctgg cgcgcggtta tagcgtcacc agcgccgctg atggcgcggt gttaaaacag
     5821 gttaagcaag tgaaagtggg tgagacactg accacacgac tgggagatgg tgtggtgatt
     5881 agtgaagtca gcgccgtaac aaaaacccgc aaatcacgta aaaaaacgtc taatccgtag
     5941 accggataag aggcgccagc atcgcatccg gcaataactc ccaggccacg ccagatccgc
     6001 catgctggtt actggaagca ggtcaccaga cccggtttat ttattagcgt gatactacaa
     6061 tcctgtgctc cggtaccttc gtccatccca cattgttgag aggacaaata aggaaaactg
     6121 ggtgaagacg gccatcccag tgaattaaaa acagtatgca acaactctaa ttcattgtat
     6181 gtcggtgcat tttctatgct gccacactgc cttgcgatat cccatttact ggcatcaatg
     6241 atatgtgcag aagcccagtt ttcgtagtta ataatcggtg aggatacggg attagccccg
     6301 gacggcaatt ctgtcgttaa caacggtcga tgtagcgatt taccattcac cactaccgtg
     6361 tctgccatat gtccccaaaa gtgcgccgag gaaacatttg ggctggttgg taccgtaaaa
     6421 atagcgtcca gcgtgtctgt cgcggaagcg taattagcca gagtaaccgt cagtggcgtt
     6481 ttataaccag gggtattatc ctggcggagc gtaaaacttg ccgtcccatc cgcgcctgtc
     6541 atgccactaa tggtattacc gctttcactc agcgtaacag ccgaaccaga taacggctga
     6601 acgatgagat cgtccatcgc caccacatca ccatacaacg ttgccccagc gttacgcggc
     6661 gatgcctcac cgcgtttcaa agtaaattcc acattcggca ccgtattccc ggcgctatct
     6721 ttcaccgtca cggtaacggg catggcttcg ccttttttca ctttcgccgc ctgagtggct
     6781 gcatcaaacg ctgttgaggt aagtgtgacg ctggaaaccg ttgaacgcgc gttagccagg
     6841 caaggctgaa atgattttgt tgaacccgta tacagcgtgc catttctcaa atcaaccgac
     6901 tggtctttac catcggcgga tttatcctct gcccaccaag tgtaatacgc aggccagcca
     6961 gtttcagtga caaacgtgtt attcggataa cgcgcagaca acgaacttaa atcattgaat
     7021 agtggctgcc ttgctacttc acaagggctc acactcgcat ctgatttctg tgttggggta
     7081 aagtacggcc agtcttcatt attgttattg atcttgccga cgccagaagt cgatgtcagt
     7141 tcactccaca atttcgggcg ttcaaatacc gcaccattca ctgccgtcaa ggttgctggc
     7201 atatgccccc agtagttagc cttcgcagaa tccgggctgg tcactaccgt gaatatcagg
     7261 tcctgattag ccgtaacctg cggatttcgc gccaatgaag ctgtaaacgg cgttaccaga
     7321 ccgacggtgt tattttgggt aacgttgaac gttatcgtgc catcactgcc ggtgaagccg
     7381 ctccatgccg acgtattctg gttgaaggag atacgcgccg atgtcggggt gacttcatct
     7441 aaaatgagat catctacggt attggaggat acagagatcc ctgaacggct tttcgcctgc
     7501 acgcgcttga gcgtaacacc ttcgccgcca acaggattac cattggcatc tttgatcgtc
     7561 accacgatgg gaagcatctc accttttttc acagatgacg tgggggtacc accattgctg
     7621 gcagttcggc cctcatcata ggcagtcgat gacagcgtaa ggcttgctgg tgccgaatgt
     7681 ggctgcgtca gacagagcat taaagcgtat ccattaatag tcgtcgtcga ttgtcccgtt
     7741 ttcaaattaa tgacgccgta ataccacgtc aacgcgtcag tggtgctcat ttcataagtc
     7801 caccaatttc cacttccggc agttataggc aaccccaaat ctgtcagtat tgtcccgcct
     7861 ggatgatcat tatatagccc ctgtaactca ttgagacgcg gctgatactc aaccgggcaa
     7921 tccggcgtaa ctttattggt ataaaaaagg tatgaccacg actcattatt attacttacc
     7981 gaggacccca aggtcgcttc tgctgccagt agcggtcgtt taaaggtcac ccccgcccta
     8041 ctggtaaacg tgtcgggcat atgaccccag tattttgctt tcggtgagtt cggactggtt
     8101 atcacagtga aaataacatc catcgtggca ctttttgtgg tgtcgcgcat caatgttgca
     8161 gtgagcggag ttgccagccc ataagtctcg ttttgcgtca catcaaaagt cgctttacca
     8221 tcacttcctg tcgtcagaag tgcctgtgcc ccattattat agagtgacat gtttactccg
     8281 gcaggggtga ccggcacgat agtaagatca tcatacgtag aatctagcct gttttttgca
     8341 cgatccaacg cagtcccgcg tatgagatta aagtttgcgc ctggcaacag atttcctgcg
     8401 ctatcccgca caatcaccgt catccggatc gccgtacctt tcgccgccgt tgcactaggg
     8461 cgaccatcgt tgctggccgt tttctccgca tccagtgccg tggaggtcat ctctatagaa
     8521 gctgccactg catgagggtt taccagacag gacacaaatg ccacggtgtt tgataatgcc
     8581 ctggagcttg atccattcga taaattaata gactgatcgt accagacgtt cgcgtaggca
     8641 attttatcgt aagcccacca ggtgttagtc agcggaaggc ctaaatcggt ccctatcgca
     8701 ccattaggat gttcactgta taactcttga aattcgctta ataacggttg ataaggttcg
     8761 ccgcagtctg atttgctgac atcatttttt gctgccgtat tcaaagaaag ccatgattca
     8821 ttattagcca tggttgactg ccccgctgga tgctcggcag taagcaatgg ccgcttaaag
     8881 gcaattcccg accgggtagt aaacgtttca ggcatatgcc cccagtaact tgccagtggc
     8941 gtatccgggc tggttatgac ggtaaacatg accggtaatt tgttggattc tatcgtcggc
     9001 tgatacaact gggcgtacat attggttacc aggcccatag aatcatcttg ttttaccgta
     9061 aaggaggtag tcccatccgc gccagtcacc ccataaagca ggcttgaaga actattcatc
     9121 ctcgcagaag cgcctgccgc gttaattacc gtaatcgact tatttatcat ctcggcattt
     9181 tgacggttct tcgagccttc acgtctgaga ataaagggga cattgggcac gctatttccc
     9241 tgcgcatttt tagtcaccac gcgaatcacc gcgtcttcgc cttttttcac tttcaacgca
     9301 ctcaaggtgt cgtcaacttt gacaaattgc gccgtatcat tcgcctcaac gatgatctga
     9361 ctggcaacgg cgacaggcgt ggtccggcag ctcagtaatg caccaccaga tgagggcatc
     9421 tgcgtttcat ctcctgtctt cagcgaaacg acatcatacg taaaggtttg agtgaacgta
     9481 tctgcaacgg tattcgagat atagaacctc gacactggcc agccaatagc atcatgtaat
     9541 gcgctactcc cctggtgcgc ataaatggcc ctaagttcat tgtcagttgg aacatactcc
     9601 gcgccacaac catttatcga ggtatcgtta gcctggcccg acgggaacgt agaccaggcc
     9661 tccccctctt ccaccagggt accgatactg gtggtagcac tattctcgtc cctcagtaaa
     9721 ggccttcgat aaataagacc gtcatcgcct gtcgccgttt cagccatatg cccccagaat
     9781 ttggcttttg gcgaatccgg gctggtaagc accgtaaaga caacgggaag cgtcgattta
     9841 gtgctgctgg aggaatccaa cgctgccgtg agttctgttt ttagcccggt agtctggtct
     9901 tgcttcaatg tgaaagtggt gatgccatca gcccccgtca cgccataaat aaccagggtc
     9961 gttgaaagaa aatcatcttg agtatttccc caggcatccg ttaccgccag cgaagcaatc
    10021 gacgtcgtag ttgacacatt ggccctgttc acgctggtgt ttcgtttgag ggtaaatgcg
    10081 gtatttccca cagggttacc ctgcgcatct ttagtggtca ctcgtaatac ggcttcttcg
    10141 ccttttttcg ctttgacaac attattcgta ctgtcaaatt ttgtggggtc tgcagcttca
    10201 agaacaattt ggcttgcctt aacaatcggc gtcgtctggc aggtaagcag ttcttttgtg
    10261 gcagatggaa ttatcgaact ggtgcctgaa ctgagattga cggccttgaa gtcacgctgt
    10321 tccaggctac tactacctgt cgtgctggaa aggtaacttg aggcgacggg ccagccctga
    10381 accgttttca tcgcattgcc gcggttggct tcatacaatg actccagccc ggcctgggag
    10441 ggaacatatt cactaccaca accattagag gatgtgctta tagcctgggt ttgggtaaat
    10501 tgcgcccagt cttcattctc ttctgcaatc gctgttcgac ccgacgtact ggataactct
    10561 ttcaacaata atgggcgttt aaagaccgtt ccatctgcag ccgtcactgt ttcaggcata
    10621 tgcccccaca ttttcgcctt atcgctgtcc gggctggtga caacggtaaa aatggtatcg
    10681 agggtcgctt ttttcgttgt gtctgaatag agcgcggcgg tcagcgacgt ttttgtgcca
    10741 tgggtatccg ggcgggtaat agtgagaatt ttagtgccat cgctgccggt catcgcgctg
    10801 tatgctgcgg cggtatcatt gagcgaagtt tcatccgcca ggccactatt gatcacaacc
    10861 ggcgcaacca gcgcatcacc gccaccggca atgtgttttt cgtttgatcg ggtataacca
    10921 tcgccacgct tcagcgtaaa aggcatatca cccaatggat tgccctgagc atcttttacc
    10981 gtcaccttca cctgtagcgt ttcgcctttt ttcagcttcg ccgcatttgc tgccgcgttc
    11041 cactgcgccg ggtccaccac ttccagcgta atgctgctgg caggattgtt agccgtcgtc
    11101 aggcaactga cataaaccgc cgtgctaccg ccaacggtcc gcgcgccatc gttgagggct
    11161 atggtgtagt aatgcggcac ctgatccgcg ggcgaactgc tccagtaggg ctgacgctgc
    11221 gttggccaac cgtgtaccgt ttgaacggcg ttaccgctgt tagcatcata gagcgcgtta
    11281 agctgggaac ggcgtgggag catattggca ccacagccac ctttactggt gctggaagtg
    11341 ttggcctggc taacgcgcga ccacacttcg ttattttccg ttaatgtgcc gtccgggctg
    11401 gacacttccg cagccagccg gggtcgggca aaggttaatg catcgacggt aatcgtctca
    11461 tccatatgac cccacatctg cgcgccaacc gcatccggac tggtgagcgt agtgaaaatc
    11521 acattgtaat taacagtatt gggaatatag gagtttacag gcgcaatatt cagcggcgtc
    11581 gctaagccca caccttgcgg ttgcttaatt tccacagtag caaaaccctg cgcatccgta
    11641 gtaccggcat agactaaaga cggttgcgat gttccgtaga gtgtgccatt catctcgatt
    11701 gcgccgcggg ttggatcatc aaaacctgta gtctgcccct gacgattttt ccccatatct
    11761 ttagtgatgg tgaacgcggt atacggcacg ggcgcattat tcaaggcatt aaaggtgcgt
    11821 accgtcatgg taatcgtttc acccacttta gcctgcgctc tgctgcttgt ggaaacatca
    11881 tgatcggtgg tcgcagcaat ctgggcgacc atctcattgc ccgaacaaga aaggtagttt
    11941 tgcttaaagc cggaataggt gtcgatgttg cccgtggcca ggttcaccga cgcgtgcgac
    12001 gtctcttcca ccgcactaag atacgctttt tgcaacgttg gccagccata ttcggtgccg
    12061 atgacattgg ctggatgtgc ggcaaacaaa ctgtgcaatg agctttggct cggaatatga
    12121 cctaaaccac actcagcctg catactgcta ttctgatcaa acagcgccca gtcttcgtta
    12181 ttttcccgca ctgctcccag ttcgttgtcg gtttcatccg ccagccgtgg gcgtttaaag
    12241 atattattcg cttcaataat gcctaacata tgtccccaca tccgcgcttt gtcggaatca
    12301 gggctggtga tcaccgtaaa aataacgtct ttctcatccg aggcggtata atcactgcgc
    12361 attttcgcgg tgatgtgtgt tttcaccccc acgccgcctg gctgggtcag cgtcagagag
    12421 gcctctccgt tagcatcagt gatcccttca taggtatgcg cgtcaacttt gcgaacgtta
    12481 ctgccaccat caatttgtac cgggtgcgtc tcccacgcag cgtcagtttg attcttacgg
    12541 ttaacaccgt catccagatg cagcgaaaag tagtagtaag ccagcggctg atcgttgttc
    12601 ttgctatcgg taatcgtcag gcgtaaagaa gcctcttccc ccacctttac tttcatcgct
    12661 tttatcgtcg aatcataact gcccggcgtc agtaccagct tcggttcaac atcgggcgct
    12721 tctttatcga cacagctcac aataaacgtc gtatcgtccg gtttttgcgc ttcgctgcgg
    12781 ttcgaaacat ccgccgcata gtgctgcccg ctcatccccg ccagtgatga ccagtagtag
    12841 ttaccctgca tcggccaacc agccacatcc tgcactgacg ttggcaccac cgtcgccagc
    12901 gcaccgaact gacgcattcc gggcaaaatg tcgcaatgtt tatccgcacc actccaggta
    12961 aaggtggacc aggtttcatt gtgatcgtta accgtaccgt cttcattatc gacttccgcc
    13021 gccagttttg gtcgactaaa agtgtaatca cgggccttca gcgtatcggg catatgaccc
    13081 cacattgtgg cctgcgggac atccgggctg gtgaggacgg taaagatcac tgccgtttca
    13141 ctggtctgag caatacccgc gaggctcgcg accaacggcg tttttacacc agggccgttg
    13201 gcctgggtga ccgtaaccgt ggcaacacca ttcgcatcgg tagtaccacg atattccgtc
    13261 gcggtggtgg ttaattcggt cgtgcccagt tttaccggcg cagtattgtt cactacaccc
    13321 tggcggtttt cagcatcttt gcgtttgata acaaaaggaa tattccccac aacattaccg
    13381 acgcaatctt tggtggtgac cgttaacgtg atactgccgc caaccgtcgt tttcgccgca
    13441 ccggaggatt cgtcatagcc cgcggcgctg acggaaataa ggtcaccctg cgcgggcgca
    13501 atcttcagcg tcactttctg atcggcaagg gtcagaacac tttcataggg atagcccgtc
    13561 gaggtaagta gatataacag cggcatggtc tgctgggggt ctggctgcgc ccaggcgatc
    13621 agcgaacgcc cggcaaggcc ctgtgcgcaa aatgagcgat ctgcgactgg ctgtgggtcc
    13681 agtaaggtgt ccggcgtcgc ggcatccgcc caaactaaag atactgtcgg tggcgtttgg
    13741 ttttcccaac gtggtgtggc aatgatatcg ccttctctgt cttgcgggtt ggtgacgagc
    13801 aatttagaga cgctaacatc cgcgctgaat tcactcggtt tagcggtaaa cgctacctca
    13861 tgcgtctttg caggatccag aaagacactg ccctggtaga cgggtatgct ggcactgtcg
    13921 gcggtgggca cggtaccgtt aaattcgccg accgtatctg aagagtcttg ccatgcagat
    13981 tctgcccatg accacccagg cagcattgcg cttatcagca atgcgcataa cacaaggatt
    14041 ttattcaggg tcagggacat gacagaaact ccaccggttg ttaaacggac gcttgtcgca
    14101 tcaacatatt ttgcttaatc cgggattaag caaaatatgc ggaaacagca ttcccttgcg
    14161 ggaatgctgc tgagtacgcg ttatttgtgt ttgtaaataa tgtgaagttt gtaaccctgt
    14221 accccgtcgc cgtttgtcgg aatggtaaga gcctctttac catcgcgcgc aggaccgtta
    14281 aggttggttc tggcttcgtc gttggtcgca gggatggcca gattgttatt atcgaagctt
    14341 gagttaacga taccacctga agtgcccagc tgctggctgc tgccagaaag tacccaacgg
    14401 aagtcataat tggcggtaac gtcagcatcg gtacccgcat caaacttgcc gtttttgttc
    14461 tcatccgagt acagtttggc cacgtaggtt ttgccaagtt cgattggcgt gctgccgttg
    14521 ataaggttga tactggtacc atcaacaaag atggcgactt tcaggttctg gttaaccacc
    14581 ggccctggcg caacgttatc gctgtcgctt ccgccgccgc tggcagtgga aatgtcataa
    14641 agatgcagcg cagtcccgac gtttggcgta ccggtctgcg tggtcggcgt aatttcaata
    14701 ccgatataac gaccacggtc gtcatcggta agagtgtaac tggtcacctt cgttgccagc
    14761 tctttcggat cgcccccctg agcgtccttg tagctcatcc agcggatagt atcgatagtc
    14821 tttgcgttat cgcccaatcc cccaagatcc acgtcgcctt cggtatcacc aatttcccaa
    14881 cttacggtta atttatcacc cgagtaaaga cgcttgtccg tttcactgcc tgtcgggcga
    14941 gcgcctttta gatcagaagt caacgttaca tggtcggcat ctgtcgtagc gcttccccct
    15001 tccgaataga gccacggaat ggttccctgg aaaatattgg tttcgccgct gtcgacccag
    15061 gcgctttctt ccgccagcgc cacaggtgcc acacaataac ctgccagtgc cagcgccaat
    15121 gcgatccggg tcagaccgcg tcttttatcc tgcttcattg atgctctcct taatgaacct
    15181 tcagcgactg gccgactggc cttacgccaa tcttattttt tggtatacag cacgcgtaaa
    15241 ccgtatccct gcaagccatc tcccgcttgt gcgccatagg tttgcgctgc ttcgcggtta
    15301 gtggccggaa tcacaatgtc ctcattggaa gtgttcgcct gtgcacccac ttcaccattg
    15361 atgctcttgc cggtaaattc ccacttgtag tcgtactgcg ccatctcttc gtcagtcagt
    15421 ttttcgacct gctggaagac accgtcttta ttgctgtcac gccacaaaac aaatcgataa
    15481 gtgttgttga gcggcagcgg ttctttatct ttacctatca ggttgctggg tttagccgcc
    15541 cccacacgat agataaatgc atttagccca ccaatttctg ccccgaggaa agtgacatcc
    15601 acatagttac tgtcgtcgta gggcgcagct tttgctttcc agcgataggt gccgggtaag
    15661 gtactggaga gggtgacagt acccttttct ttatcttgtt tagcgataaa aacgcagggg
    15721 ttgtcggtcg agcaaggctt gttggttaac gtcactcgtt tgtcctgcgc gtcgtaaagc
    15781 cgcggcgtga ggttatcttc cggcagcgca tcgccgttgc ggtcggtgtt atcgccaaag
    15841 ctgtcggtta catcgagatc catcaccaca ccatcaacgc catctgcctg ggcgctggct
    15901 tcgtggctgt ggttgttctc ttccggcacg ttcgcgatgt tattaacaat caactcaatt
    15961 ttacggtcct gctgaacggt gatttccacc acaccagagt tctgtcggtt gcccttttca
    16021 tcttccagcg ccagacgtac tcgccaggta ttgagttttt cttgttcggc tttgtcagag
    16081 ctgtactgcc aggcgggcag caccaggttc cagtggttat tgttaccctc tgtcagggta
    16141 ttctctttga taaccggcat aaccagcgtg cgggcagcat tgtttttcgc ctgccatgac
    16201 gcagcgacaa ttttgtagtg gtggttgata agcgcgttaa tggtccactt acatttttcc
    16261 agcccgaccg ccgcttcagg cgtgtcttta atgacacact cggggtgttc gttggtggca
    16321 tccgctttta accacagggt gacatcaagc ggatcttttt ctttgtattc cagaacaata
    16381 aagttattac gctcaacaaa atcatgacga ctgcccatca acgagtgagc cgctttcact
    16441 ttatcgctat ccaattgatc ttttagcgga acgccgaact gataattaag agtggcatta
    16501 atgctgacat cgctgttatc tccggtcccc gctttatagt ccgccccaac ggttagtaat
    16561 ggcaccggtt gataattcag tccaagtgtt accgcgtagg gatctttctc caaattatcc
    16621 gtaccaaaaa gggcaacttc atcgccgtaa tattgctcga agacaacttt gccccccagt
    16681 tgcggataag aaggtaacca ggcttcggcg cgaatatccc agccgcgcgc aggacgctcc
    16741 tcataaaaat cgaaatcttc ggagtctttc cagtcagaaa gcggatgata atagttgcca
    16801 gagaatttta aataatcagt ccacgcttcg ccgcctaaac ctaaacggcg atgtcctcgg
    16861 gtaaaatcat aatcataaaa tatatttccg cccaataacc attcatcaaa attatatctt
    16921 accccaagac caatattccc gattgtacga tcttcttttc gctgcgcaga aaattgactg
    16981 aaataaacag tggtttgatt atcgtaccag ggaacaaaat aatcgatgga actgccatcc
    17041 agatcgccgc cctggccaat ggagaggttc gaacggacct taccgtaggg agagagagtg
    17101 tcttcaatat aagattgtgc ggttgaggtg atttgacttt gcagatagct tctggcctga
    17161 gattccagcg cctcaggggt caagttttca aacccttgcg tggcggagtt gatgatgtaa
    17221 tcggctccgc gctctttcag cgatttaccc ttgttggttt gttcatcctg ctgtgctgcc
    17281 tggctaccta aatcaggtaa cgattcgttt tgcttaacat ttatctctgg cgccgcgaaa
    17341 gcagcaccag agataaatgt tagaataata cagcgtttcc atcgcagcat gcactttccc
    17401 tgtgacaatg atacagtcaa aaataagctt taatatt
//
LOCUS       DAZGAP010000113        17384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGAP010000113 DAZGAP010000000
VERSION     DAZGAP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.114"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..1427
                     /gene="tnaA"
                     /locus_tag="ACHLAM_003264"
     CDS             12..1427
                     /gene="tnaA"
                     /locus_tag="ACHLAM_003264"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO7854743.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1519..2766
                     /gene="tnaB"
                     /locus_tag="ACHLAM_003265"
     CDS             1519..2766
                     /gene="tnaB"
                     /locus_tag="ACHLAM_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO7854744.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2898..4073
                     /gene="mdtL"
                     /locus_tag="ACHLAM_003266"
     CDS             2898..4073
                     /gene="mdtL"
                     /locus_tag="ACHLAM_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO7854745.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4048..5007
                     /gene="yidZ"
                     /locus_tag="ACHLAM_003267"
     CDS             4048..5007
                     /gene="yidZ"
                     /locus_tag="ACHLAM_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO7854746.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5164..5913
                     /gene="yieE"
                     /locus_tag="ACHLAM_003268"
     CDS             5164..5913
                     /gene="yieE"
                     /locus_tag="ACHLAM_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO7854747.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5935..6501
                     /gene="yieF"
                     /locus_tag="ACHLAM_003269"
                     /gene_synonym="chrR"
     CDS             5935..6501
                     /gene="yieF"
                     /locus_tag="ACHLAM_003269"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO7854748.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6555..7892)
                     /gene="adeP"
                     /locus_tag="ACHLAM_003270"
     CDS             complement(6555..7892)
                     /gene="adeP"
                     /locus_tag="ACHLAM_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO7854749.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8059..8724
                     /gene="yieH"
                     /locus_tag="ACHLAM_003271"
     CDS             8059..8724
                     /gene="yieH"
                     /locus_tag="ACHLAM_003271"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO7854750.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8791..9264
                     /gene="cbrB"
                     /locus_tag="ACHLAM_003272"
     CDS             8791..9264
                     /gene="cbrB"
                     /locus_tag="ACHLAM_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO7854751.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(9346..10068)
                     /gene="yieK"
                     /locus_tag="ACHLAM_003273"
     CDS             complement(9346..10068)
                     /gene="yieK"
                     /locus_tag="ACHLAM_003273"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7854752.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLHNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(10083..11252)
                     /gene="yieL"
                     /locus_tag="ACHLAM_003274"
     CDS             complement(10083..11252)
                     /gene="yieL"
                     /locus_tag="ACHLAM_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO7854753.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11279..12895)
                     /gene="bglH"
                     /locus_tag="ACHLAM_003275"
     CDS             complement(11279..12895)
                     /gene="bglH"
                     /locus_tag="ACHLAM_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO7854754.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(12981..14375)
                     /gene="bglB"
                     /locus_tag="ACHLAM_003276"
     CDS             complement(12981..14375)
                     /gene="bglB"
                     /locus_tag="ACHLAM_003276"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO7854755.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14394..16268)
                     /gene="bglF"
                     /locus_tag="ACHLAM_003277"
     CDS             complement(14394..16268)
                     /gene="bglF"
                     /locus_tag="ACHLAM_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO7854756.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     MPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16402..17238)
                     /gene="bglG"
                     /locus_tag="ACHLAM_003278"
     CDS             complement(16402..17238)
                     /gene="bglG"
                     /locus_tag="ACHLAM_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO7854757.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIVKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4413 a         4388 c         4066 g         4517 t
ORIGIN      
        1 aggattatgt aatggaaaac tttaaacatc tccctgaacc gttccgcatt cgtgttattg
       61 agccagtaaa acgtaccact cgcgcttatc gtgaagaggc aattattaaa tccggtatga
      121 acccgttcct gctggatagc gaagatgtgt ttatcgattt actgaccgac agcggcaccg
      181 gggcggtgac gcagagtatg caggccgcga tgatgcgcgg cgacgaagcc tacagcggca
      241 gccgcagcta ctatgcgtta gccgagtcag taaaaaatat ctttggttat caatatacta
      301 ttccgactca ccagggccgt ggtgcagaac aaatctatat tccggtactg attaaaaaac
      361 gcgagcagga aaaaggcctg gatcgcagca aaatggtggc gttctctaac tatttctttg
      421 ataccacgca gggccatagc cagattaacg gctgtaccgt gcgtaacgtc tatatcaaag
      481 aagccttcga tactggcgtg cgttacgact ttaaaggtaa ctttgacctt gagggattag
      541 aacgcggcat tgaagaagtt ggcccgaata acgtgccata tatcgttgcg accatcacca
      601 gtaactctgc aggtggtcag ccggtttcac tggcaaactt aaaagcgatg tacagcatcg
      661 cgaagaaata cgatattccg gtggtcatgg actccgcacg ctttgctgaa aatgcctatt
      721 tcatcaagca gcgtgaagca gaatacaagg actggaccat cgagcagatc acccgcgaaa
      781 cctacaaata tgccgatatg ctggcgatgt ccgccaagaa agatgcgatg gtgccgatgg
      841 gcggcctgct gtgcatgaaa gacgacagct tctttgatgt gtacaccgaa tgcagaaccc
      901 tttgcgtggt gcaggaaggc ttcccgacat atggcggcct ggaaggcggc gctatggagc
      961 gtctggcggt aggtctgtat gacggcatga atctggactg gctggcttat cgtatcgcgc
     1021 aggtgcagta tctggtcgat ggtctggaag agattggcgt tgtctgtcag caggcgggcg
     1081 gtcacgcggc attcgttgat gcaggtaaac tgttgccgca tatcccggca gatcagttcc
     1141 cggcacaggc gctggcctgc gagctgtata aagtcgccgg tatccgtgcg gtagaaattg
     1201 gctctttcct gttaggccgc gatccgaaaa ccggtaaaca actgccatgc ccggctgaac
     1261 tgctgcgttt aaccattccg cgcgcaacat atactcaaac acatatggac ttcattattg
     1321 aagcctttaa acatgtgaaa gagaacgcgg cgaatattaa agggttaacc tttacctacg
     1381 aaccgaaagt attgcgtcac ttcaccgcaa aactgaaaga agtttaatta aaacactcag
     1441 agtggctata aggatgttag ccactctatt accctacatc ctcaataaca aaaatagcct
     1501 tcctctaaag gtggcatcat gactgatcaa tcagaaaaaa aacactctgc attttggggt
     1561 gttatggtta tagccggtac agtaattggc ggtgggatgt ttgctttacc tgttgacctt
     1621 gccggagcct ggtttttttg gggcgctttt attcttatta tcgcctggtt ttcaatgtta
     1681 cattcagggc tattattatt agaagcaaac ttaaattatc ccgtcggatc cagttttaac
     1741 accatcacca aagatttaat cggtaacacc tggaacatta tcagcggtat taccgttgcc
     1801 ttcgttctct acatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
     1861 acgatatcaa tgaatttggg ttatcatgct aatccacgta ttgtcgggat ctgcactgcc
     1921 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgtat tacctcattg
     1981 ttccttgggc tgaagattat ctcctttgta attgtttttg gttctttctt cttccaggtc
     2041 gattactcca ttctgcgcga tgccaccagc accacggcgg gaacatctta tttcccgtat
     2101 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
     2161 ctgattattt gctatggcaa acgcaaagat aagctaatca aaagcgtggt cttcggttcg
     2221 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tatcccgcga
     2281 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
     2341 ctcggcacca aacagcatgg cattatcgag ttttgcctgc tggtgttctc caacttagct
     2401 gttgccagtt cgttctttgg tgtcacgctg ggattgttcg attatctggc ggatctgttt
     2461 aagattgata actcccacgc cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
     2521 gcgttgttgt atctgatctt cccgaacggc ttcatttacg ggatcggcgg tgccggactg
     2581 tgtgccacca tctgggcggt cattatcccc gcggtgctgg caatcaaagc tcgcaagaag
     2641 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
     2701 tttggtataa ccgtaatttt atgctggttc ggtaacgtct ttaacgtgtt acctaaattt
     2761 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
     2821 tgtccaaatg gcaactcgcc tgatcctcct tcaccacgta tgctttgcgt caccttacta
     2881 tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg gttttacttt
     2941 atcccgcagg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
     3001 ccagcgaagc gcagttgcat attgcgttct ccgtttatct ggcggggatg gcggctgcga
     3061 tgttatttgc cggtaaagtg gccgatcgtt cagggagaaa gcctgtcgcc atacccggcg
     3121 cggcgctatt tattatcgcc tcggtgttct gttcactggc tgaaaccagc gcgttatttc
     3181 ttgcagggcg atttctacag ggactgggcg cgggctgttg ttacgtagtg gcgttcgcca
     3241 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcgtta ctcaacggca
     3301 ttacctgcat cattccggtg ttagcgccgg tgctcggaca cctgattatg cttaaattcc
     3361 cttggcagag tctgttctgg acgatggcaa cgatgggcat cgcggtactg atgttgtctt
     3421 tgtttatttt aaaagaaacg cgccctgcgg cccccacaac ttcggacaaa ccacgagaaa
     3481 atagcgagtc actgcttaat cggtttttcc tcagccgtgt tgttatcacc accctcagcg
     3541 tttcggtgat cctcaccttc gtcaacacgt cgccagtatt gctgatggaa atcatgggtt
     3601 ttgagcgcgg agaatacgcc accattatgg cgttgaccgc tggcgtcagc atgaccgttt
     3661 cattctccac gccgtttgcg ctgggaattt ttaagccacg tacgttgatg atcacctcac
     3721 aggtgttatt cctggcggcg gggatcaccc ttgccgtttc accttcccat gcggtttcgc
     3781 tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggtgtg gcgatgagtc
     3841 aggcgttagg gccgttttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
     3901 aagtttgcgg ttcgtcactg tggatttggc tggcggcggt ggttggtatc ggcgcatgga
     3961 atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg attatgttcg
     4021 tcgcgcctgg acgcccggtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
     4081 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
     4141 atgaacgtga ccccttcggc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
     4201 gacccgctct ttgtgaactc accgctgggg ctgtcgccca caccgctaat ggtcagcatg
     4261 gagcaaaatc tggcggagtg gatgcaaatg agcaacctgc tgctggataa accgcaccac
     4321 cagacaccgc gcggcctgaa gtttgagctg gcagcagaat caccgctaat gatgatcatg
     4381 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaattacgt
     4441 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
     4501 ggtcgcgaaa gtcatccccg ttcgcgggag ctgttaagct cgctaccgtt agccattgat
     4561 tatgaagtgc tgtttagtga tgtgccctgc gtctggttac gcaaagatca tccggcactg
     4621 catgaagcat ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
     4681 cagagcgata cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt
     4741 gctatgagcc tgccggaatt cgagcagtca ctgtttatgg cagcacagcc cgacaatctg
     4801 ctactggcga ccgcaccgcg ctactgtcag tactacaatc aactccacca actgccgttg
     4861 gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt cccttttacc
     4921 ctgctgtggc ataaacggaa cagccataat ccgaagatcg tctggttacg ggaaaccatt
     4981 aaaaatcttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc aatttcacaa
     5041 taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg
     5101 tccatcattc cagtaaatga ttactcttgt attcataatg gaccattaag catggagcga
     5161 aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt
     5221 ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt
     5281 ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg
     5341 ccggaaatcg tcaccctgcc gaaaggaaaa ccggttttca gcgataaaaa tttgccttcg
     5401 ttttctattt cctatgccgg gaatatggtc ggcgtggcgt taacaaccga aggtgaatgt
     5461 ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat
     5521 aaccacacct tttccagcaa tgaatcgcta tggatcagta aacaaaacga tcctaacgaa
     5581 gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg tgatgttttg
     5641 aatgacgatc cgcgcgatct gcaactactg cccattgccg gacgcctgaa atgtgctcat
     5701 gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc tggtgtggtc cgtggcggtc
     5761 acgcccacga ttgaaaagct cagtgtctgg gagttagatg gcaaacatgg ctggaaaagc
     5821 ctgcccgata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg ttttgcccag
     5881 ctctctaccg tgaaggcttt ttcgccaaat tgatagacaa ccacaggagt catcatgtct
     5941 gaaaaattgc aggtggttac gttactgggg agcctgcgca aaggctcatt taatggcatg
     6001 gttgcacgta ccctgccgaa aattgctccg gcgagcatgg aaatcaatgc gttaccttcc
     6061 attgccgaca ttcccctgta tgacgctgac gtacagcagg aagacggttt tccagcaaca
     6121 gttgaagctc tggcggaaca gatccgtcag gctgacggtg tggtgatcgt cacgccggaa
     6181 tataactact cggtaccggg tgggctgaaa aatgccatcg actggctttc ccgcctgccg
     6241 gatcaaccgc tggctggtaa accggtattg attcagacca gctcaatggg cgtgattggc
     6301 ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc aatggtgatg
     6361 aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatccgca aaccggggaa
     6421 gtgattgatc agggtacgct ggaccacctg accgggcaat tgaccgcatt tggtgagttt
     6481 attcagcgag ttaagatcta aataaaaaac ccgccagcaa tcatgcatgg cgggttttta
     6541 acgcgctatc gattttagtg agcgtcgata aagacaatct tcaggataaa cagcagcgca
     6601 acgatgatta cgcacgggct aaggtcacgc agacgtccgg taccaatctt catcacgcag
     6661 taggagataa agcccagcgc gataccttca gtaatcgaga agctgaacgg catcatcaca
     6721 gcggtaataa acgccggaac agattcagta agatcctgcc agttcacgcg agcaagactt
     6781 gaggtcatca acacgccaac gtaaatcagc gcaccagctg cagcgtagcc tggcaccatc
     6841 cccgccagcg gcgacaggaa gataaccagc aggaacagca gaccaacaac cactgccgtc
     6901 agaccggtac gaccgccaac ggaaacgccg gaagaagatt caatatacgc cgtaacagaa
     6961 gaagtaccga taaacgaacc ggtcaccgaa gagatactgt cgacatacag cgcctgcttc
     7021 atgcgcggga atttaccttt ctcatcagcc agacctgctt tatcggtcac gccaatcagc
     7081 gtaccggagg agtcaaacaa gttgaccagc atgaaagaga aaatcacccc tgccagccca
     7141 aggttaaacg acccggctaa atctacatga cccacgacac tcattacgct cggcggcgca
     7201 gaaacgatgc cattatagtg gacatcaccc agcatccagc ccagcagcgt tgtcaccacg
     7261 atggaaacca gcaccgccgc gtgaatattg cgcgaggcca gaatggcgat gatgaagaag
     7321 ccgaggatcc ccagcagtac gctgtgagaa gtcagattac cgatgctcac cagcgtttcc
     7381 gggttagcga caatcacacc tgcattcttc agtcccatca tgccaataaa caggccgata
     7441 ccgctggtaa tgcccacacg cagactcacc ggaatgttgg ctatcatcca gtagcgaacg
     7501 cggaaaatcg tcagtaacag cagaccaatc gcgccccaga agattgcgcc catcccgacc
     7561 tgccacggca agcccatcgc ctgcacaacc acaaaagcga agaacgcatt caggcccata
     7621 gcgggtgcca gtgcaactgg caggttagca aacagtccca tcataatact gccgaatgca
     7681 gcaatcagac aggtagtaac gaagacggcg ctggtatcca tgccagcaac gccaagaatt
     7741 tgcgggttaa caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc ggcgatcact
     7801 tcggtccgtg ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag catcccctgg
     7861 ccagaagcct gggttgtgtg ttgttgactc attatctatt tccgaacaag gagggaaaat
     7921 ccgtcgctat cgtataccaa aatgagacaa tatggacggc tgaagataac tttttttcat
     7981 tgtatttgct tatacggcaa cgattgcgtg atgcgaattg gcatgacgaa aacgttaaac
     8041 taacgataaa ggaaagacat gtcccagata gaagcggtat ttttcgactg cgacggtacg
     8101 ctggtcgata gcgaggtcat ttgctctcgc gcctatgtca cgatgttcag ggaatttggc
     8161 attcacgttg acctggaaga gatttttacc cgttttaaag gcgtaaaact ctacgaaatc
     8221 atcgacatca tcagtaaaga acagggcgtg acgatggtcc aggccgatgc cgagcacgtt
     8281 taccgcgcag aagtagcacg tctgtttgat accgaacttg aggacatcgc aggagcgaac
     8341 gaattgctgg cgagcattaa cgtgccgatg tgcgtcgtct ctaacggtcc ggtcagcaaa
     8401 atgcagcatt cgcttggtaa gctcaacatg ctgcactact tcccggaaaa actgttcagc
     8461 ggctacgata tccagcgttg gaagcccgac ccggcgttaa tgttccatgc ggcaaaagcg
     8521 atgaatgtga acgttgaaaa ctgcattctg gtcgatgact ccagcgccgg tgcgcaatca
     8581 ggaatcgatg ctggtatgga agtgttttac ttctgcgctg acccgcacaa taagccgatc
     8641 gttcatccga aagtcaccac ctttacccat ctttcgcaat taccggaact gtggaaggcg
     8701 cgtggttggg atattacggc atagttattc acactccctt cacttactcc acttaaattg
     8761 gcacgcaaag gtaagtaaag ggagtttgat atgtctgttt cacgtcgggt aattcatcac
     8821 ggactgtatt ttgcggtttt agggccgtta attggtgttc tgtttcttgt cctctacata
     8881 ttcttcgcaa aagaaccgct gattcttttg gtaataatac aggtacttcc attgtttctc
     8941 ttaatgtcga taactacggg agctattcct gcaatgttaa ctggcgtaat ggtcgcctgt
     9001 ctgccggaaa agatcgggtc acagaaacgt tatcgttgtc tggttggcgg cataggtgga
     9061 gtcgttatca ccgagatcta ttgcgctgtt atcgtacata ttaaggacat ggcttcctca
     9121 gcgttgtttg aaaacattct ttctggtgaa aatctcgttg tccgcatcat cccagcattg
     9181 ctggcaggcg tagtgatgag cagaatcatt acacatctac ccggattgga tatttcatgt
     9241 cctgaaacag actctttaag ctaagcggaa tgctttatct atttgtcatt aaatgttgga
     9301 gaacaggccg gagcgtaatt cacacatccg gccttatttt taagcttaac cgagcgctaa
     9361 ttctgcggca gcggctttat cagcgatgac tatcagcgaa gggtgcaact gcaatacgga
     9421 agcaggaaca tcttcggtaa ccggaccttg cagaacattt ttcagtgcct gcgctttacc
     9481 cgcgccactg acaattatca gcaagttttt cgccgccatg atgcttttcg gccccatcgt
     9541 cacgtagctg tcaggcacta atgaaaagtc gccacccaac tcgccatgcg ccacaatatc
     9601 aaccatctct ccctggatcg ggaactccac ggtttgctca tggaaatggg tcgtatttgg
     9661 cagattgcca caaaaatgac catctacacc taatcccaac accaccaaat ccaggccgcc
     9721 ttcacgcgcc agtttctgat catgctcgcg gtagttatca atagtgagct tctggatatt
     9781 ctcttctttg atccctgcag gggtgaaaaa cagattatgc agattggtaa tcgttacgcc
     9841 ttccccttct ttaccgcgaa atggaatttc atcgaaattg tagaaatagc agttatcgta
     9901 ccagggctta cctttaacca gagtgatgag atattcgtac atgcctttgg gcgtgctccc
     9961 ggcggtaatt gccaggttaa cgcgacgcat ctttgacata taaccaagca gatgatgtgc
    10021 cgcgacacgg ctcatttcct ggtaatcttc ggtaatgatc aatttcatct taatgcctta
    10081 tattatttga ataatttctg tacaaaggct gcataggctg gtcgccagac atccatttca
    10141 tggttcagac cgggatattc ctggtaatca aacttaattt ttttctgctc aagctcagtt
    10201 ttcagcccgg cgatatcctt gccggttacg acatctttat cgcccacgac cacagtaaaa
    10261 ttacgtagtt gctggttaat agctgccggc tcgttcagcc gggcagcgac cccttcatct
    10321 ggtacggtcg tcgtggtaac accactgaat gtggccagcc agccaaagct ttccagatga
    10381 ttcattcccg ataccagtgc ctgataccca ccttgtgaaa gccctgccag tgcgcgacca
    10441 tcggcatctt tacggacatt aaaacgcttg ctaatcagcg ggataatatc gttcatcaat
    10501 tcgcgatctg ccgctttagc atttaacgga taaaagactt tacgtctctc ctgaggaacg
    10561 aaatcttcgg gaataatgcc cttcgcatcg gtttcggtat ccgggatcac caccagcatc
    10621 ggtttaattt tcccttcagc cagcaggtta tccatgattt gcgggatacg cccctgatca
    10681 atagcggaac gtccggtatc accaaagccg tgatagaaat agagcactgg caaaggctcg
    10741 cctatgccgg tgtatcctgg cggggtccag acatacatct gacgttcgga ttgcaatgcg
    10801 ttggagtggt aagttatggc gatcaaatca ccatgcacaa cagaacgtgt atccagataa
    10861 ctgcccggta ccagaatcat actggagtta acctggcgct gaggcttagt cattgctgta
    10921 cctgtatcaa tgctgcgtac accatcaaca ttgaaaaaat actcgtacag gttgcctttc
    10981 agagcaggcg tgcgccacga ccagactccc gcttcgtctt tggtcatcgg gtgaatattg
    11041 tccggaaccg gaacacccac cactacagag acattttttg ccccaggggc aaagtagcga
    11101 aaggtcacgc tgttatcggt attgacctga gtcacatact gtttcaccgg aatagtgggt
    11161 gccgggctgg ctggcatatc cgctgctatg gcccattgag cagaaatacc gctggcaata
    11221 gccagcgtta aagcagcaat ttttatattc atcgtaatat tccttttatt aagttccatt
    11281 accaccagat ttcagcctgg gcaccgacag caaactggtc gtctttattg tcttcaaagg
    11341 tgaagccatc cagttcgttt tccagagctt tgatatatgt agcgtagaaa cgaatttccg
    11401 ggcgcgaggt caacatactg gtattgactt taaaggtatg gaagagcgtg gttttataac
    11461 cagactcatt atatttattg ctattcgcat ctttgttttg ctgggtgaaa tagcccagtt
    11521 caacacctgt ctggttatat tggtcccaaa tataggccgg gcgaacaacc gcacgaatag
    11581 attcgaaatc agaatgtgcg cctgtttcgt agctataaat atcgttaccg aaggagtaaa
    11641 caatcgcgtt agccacaatg aaatgatcgc cgatataagc ttcgccctgc gaagtcagac
    11701 gaactgcagt tccgcctgtg tgatcaccat aataacgacc attaaaggta gtaaatggac
    11761 ttgcaccagc ataacgacca aagttactgg cgatagagtt attcgcaacc aggaaggaga
    11821 attcgttgaa gccaccctta tcaaatttct gcgttaaaga tgtaccaaac atccaggtat
    11881 ctttccagtc ataatacccg ttattatcct gattatcttt ttccgatgcg ctttcatttg
    11941 ctgagacata acgaccgctc accattaagg tggctttatc ccataacggg atatctttat
    12001 agcgtaaatc aatggtattg gtattaatct gctgtttgtt ttgcaggctg cgatcgtaat
    12061 catcgacatc ttcacgaacc agcgcgatat caattttacc cggaccgact ttccagtttt
    12121 ccagacctac acctgcggca gcatcggtac gctgcgtttt ccagtcaagc atctggattt
    12181 caattttcgg cgcaccgtgt ttacccaccc agaaatcagc ctctggcgca aagggcagga
    12241 aacctttggt ggtaacgtac atatcagaga actgcataaa gttctcacca ccggcgttat
    12301 cgccaaacca gccagtagag tactgctgac caacgttacc atccatcatc acgatggcat
    12361 caacccgttt gccgttttcg ttgtagacac gttgttttaa ctgcaaatca aaccagccgg
    12421 agtattcgtt accaaagcgg cccagagaac caattgccca tgatttaggt gaaccatgag
    12481 aggctgttcc ccaaccagaa cggtagtagc cgttgtaact aaagccgatt tcatctttca
    12541 caaatttact gaaatctttc agagtcatgg aagaataaat cgagcctgtc tcactggcat
    12601 ttttctcttc atttttaacc agtacagcat caggctttgc ggcacgactg gtcgggaatg
    12661 gattggcggc atacccttga tcattcgtac ttacgctacg attcaccgtc gctggcgtat
    12721 actttttctt ctcttcatct ttatattttt tcagttcgct ttgcgtttct cttaacgcct
    12781 tttctaatag ctcaaggcgt tgttccactg ttaatgattc agccaatgat tgtgcggaaa
    12841 aggctaacgg tgccattaat aagatggcag aggtaataag atttcgtcta aacataatat
    12901 ccctttatgg tgcaaagaaa gaattaacgc atcgcatcca gactgttctg aatgcgacga
    12961 taattaaggt gctttaatgg ttattttttt aatgacagcc cccgagtttt gatcacttct
    13021 gcataccatc cgaagctttt cttgcgcgtt cttgtgaggc ttccttcacc attatcatca
    13081 cgatccacat aaataaagcc gtagcgctta gacatttgtg aatgagatgc actgactaaa
    13141 tcaattggcc cccaactggt gtaccccata atatccacac catcggcaat cgcttcattt
    13201 acctgtacca ggtgatcgtt taaataggca attcgataat cgtcctgtat cgaaccatcc
    13261 acttcaacgc tgtcttttgc gcctaatccg ttctcgacaa taaataacgg tttttgataa
    13321 cgatcccaaa gcgtatttaa cagaatccgt aatccaaccg gatcaatttg ccacccccac
    13381 tctgaacttt tcagatgcgg atttgggatc atattcagta tgttgccctg cgcattttta
    13441 ttaatgcttt cgtcgtggga aacacaaccg gtcatgtaat aactaaaaga gatgaaatct
    13501 acggtatgtt ttaaatcttc tgcgtcactt tcagtcatct caatggtgat attgtggtcg
    13561 cggaagaaac gctgcatata gccgggatac tgaccacgcg cctgaacatc accaaagaac
    13621 atccagcgcc ggttctcttc catggcctgc aacatatcct gtggctggca ggtgaggggg
    13681 taaaccagcc caccgagaag catattgcca attttcgctt cggggatcag gctatgacag
    13741 gctttaactg cccgcgcact ggcaaccagt tgatgatgga tcgcctgata aacttccgcc
    13801 tcgccactct cttctgccag ccccactccc gtgaatggcg cgtgtaatga catgttgatt
    13861 tcattaaatg tcagccataa cacaacttta tgttggtagc gggtgaagac cgtgcgggcg
    13921 taatgctcga agtgatcgat gaccgcccga ttagcccaac cgccgtagtt tttcaccagc
    13981 ccatatggca tttcgtaatg ggataacgtt accagcggct tgatccccgc ctgcgccatt
    14041 tcatcaaaca gccgatcgta aaacgctaac cccgcttcat tcggttcggc ttcgtcgccc
    14101 tgagggaaaa ttcgcgccca ggcaatggaa atacgcagac aggtgaagcc catctcggca
    14161 aataacgcga tatcttccgg gtaacggtga taaaaatcga tggcgacatc tttgatattc
    14221 tctttcccca ggatgcgcgg ttccattttt cccattacgc catgaggctg taaatctgag
    14281 gtcgtgatcc ctttgccatc ttcctgccag gcgccttcca cctgattggc agctgttgca
    14341 ccaccccaaa gaaatgtttc tggaaatgct ttcataatta actcctttta tcgttagcga
    14401 atgatggata acagcggctc accagcgttt atctgcgccg tgccgtgggg taatacgtcc
    14461 gtaaaatcat cgctattact gattaatacc ggcgtcgtca gatcaaatcc ggcctcgcga
    14521 atagcaggga tatcaaaaga aatcagccga tcgcctgtat tgaccttgtc acccacgttg
    14581 acgtgagcgg aaaagaattt gccgtccagt tttacggtat cgataccgac atgaatcagg
    14641 atctccacac catcatctga ctcaatgcca atggcgtgta atgtggcgaa caacgaagca
    14701 attcgacctg ccaccggaga acgcacttca ccaaccgagg gcataatggc aataccttta
    14761 cccaacaggc cgctggcaaa cgtggtatca gcgacgtgaa tgagcggcac aatctctccc
    14821 gtcatcggtg aacagatacc gccctgctca ggtgtaataa cctctggtgt tttctcttgc
    14881 tgggcaactt gcgctgactg actcttagcg gtgatgaaat gaaacatcac cgtaccgaca
    14941 aatgcgcaac cgatggcaat gacaccgcca ataacgctgg cccatacggt gaaatcaatc
    15001 cccgttgacg ggatggtttg catgaaggtg aaaatacctg gcaaaccaaa ggagtaaact
    15061 ttcgtttgcg cgtagccaat gatggtggcc cccaatgccc cacttatgca ggcgataacg
    15121 aaggggtact tacgcggcag attgacgcca tataccgctg gttcggtgat accaaacaaa
    15181 cctgtcaacg ccgctgatcc cgccaccact tttttctgtg catcgcgttc gcagaggaag
    15241 acgccgagcg ccgccccgac ttgcgccata atggcgggca ttaacagcgg gatcatggtg
    15301 tcgtagccca gcacggtgaa gttattgata attaaaggta tcaaacccca atgcagaccg
    15361 aacatcacaa agatttgcca gaagccgccc attaccgcgc ctgcaaatgc aggaaccgcc
    15421 tgataaagcc agagataacc ggcagcaatc agttcactta tccaggttga tagcggcccc
    15481 accagcagaa atgtgatggg cgtgatgacc atcagacata gcaagggtgt gaagaaattt
    15541 ttgattgccg aaggtaacca cgcattaagt cggcgttcca gaatgctgca caaccaggca
    15601 gaaaaaataa tgggaataac cgatgacgag taattcaaca atgtgaccgg aatacccagg
    15661 aaatccaggc ccagcgcatc cactttttgc ccgttctcga aggcagtcag aattaatgga
    15721 tgcactaacg ctccgccaat caccatggca gtaaatggat tgccgccgaa gcgtttccct
    15781 gcggtgtagc ccaggattat cgggaagaac caaaacaagg catcactggc gctaaataaa
    15841 attaaataag taccactttg ttcggtcgtc cactgaaaag tgagtgccag agccaacata
    15901 cctttcagga tcccggttgc cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata
    15961 acataaacaa agcggtttaa cagattacct ttatcatcat tttccggtgc ctgttgcgct
    16021 ttttcgccaa ggcctgccac gctgttaacc gccaggaaga catcggccac atggttacct
    16081 atgacaacct gaaactggcc accgctttcc accaccataa taataccggg ggtctttttc
    16141 agtacctcag cttgcgcttt gctttcatcc tttaacttaa aacgtaatcg cgttgcacaa
    16201 tgcatcagac tcacaatgtt atctgcgccc ccgactcctg cgactattgt tctggctaac
    16261 tccgtcataa cttgccctct accgctttgc ggcaaaactc caaaaaaaaa cctgaaaaaa
    16321 acggcctgac gtgaatcaag caattttttt caggttttgc ccgcttagtg cggtaacaat
    16381 cctttattca gtaataatat ttcagtgttc tttgcgcacg cgctctatat ttatggctaa
    16441 aaacataatc tctgcaggtg agattttacg ttgatactgc aaaccaataa aaatggcgat
    16501 ccgctccgca cattgccatg cttgcgggta attttgcttt actgcttgtt gtaatgattc
    16561 atcactatcg ttaatcgatg catgttcaat aatacgccag gataaaaatt tcagatgcgt
    16621 aaccagtcgc tgataactca agctttcttc ctggtaatta aggctgaact gaaattttat
    16681 taattgcagc atttctcgca ttaattgcgt gacacctgca acatcctcca tatttccgct
    16741 catttgggca ctgaccagat gcatggcaat aaagcccact tcatctttcg gtaactgcac
    16801 gcccaaccgt ttaacaataa tggttaacgc ttcttcccct agctggaact ctttcgggta
    16861 aagtcgctgg atatcccaca gcagcgggtt gggcagtagc acgttttgct gaaagcgttt
    16921 aatcgcaaac tggcaatggt cagttagcga gatataaata ctgtcctgca actttcccag
    16981 gcgctcctgc gccagagaga taatacgatc acaggttgcc atcacctcaa gaggcatatg
    17041 acttaagagt tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat actctttttc
    17101 tattccactt gagttaattc tttcgcctgg gcgtttttga aagccaattc cgcgccccat
    17161 gacgactttt tcccgctgtt gatcatcaat aaccaccaca acattattgt tgagaatttt
    17221 ggtgatttgc atgttcatag caaggacctt ttttataaac aaaaaaaccc gacttcacca
    17281 gtattctctg gttatgtcag gttttgcctg cgaatgcagt aacaatccag tcattaatta
    17341 atggttttta taacgaacat ccaggttcga aaattaattt agtt
//
LOCUS       DAZGAP010000114        17294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGAP010000114 DAZGAP010000000
VERSION     DAZGAP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.115"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            224..583
                     /locus_tag="ACHLAM_003279"
                     /pseudo
     CDS             224..583
                     /locus_tag="ACHLAM_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
     gene            592..1041
                     /locus_tag="ACHLAM_003280"
     CDS             592..1041
                     /locus_tag="ACHLAM_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854758.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVVHQLLLWNMV
                     DSLIKTLIAILTIPLVFWFMKKQCQRVETGKIGDEGYSWERGNPKYRPTMVWDSKGDI
                     NLLIMPLVGVLTLWGIFIIGVVTNMTWLKIWLAPKLYLIEYAASLVK"
     gene            1095..1421
                     /locus_tag="ACHLAM_003281"
     CDS             1095..1421
                     /locus_tag="ACHLAM_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854759.1"
                     /translation="MKVLMVYENVPESTEIYIFDANEDEVNDLKLSHGNYTNANCDES
                     IEKALSRVLVRISDPEHCDDDWLSYCGAVKTDAGKWSKSKVDNSTPIIMKDSDIEMVI
                     ITGMIM"
     gene            1618..1788
                     /locus_tag="ACHLAM_003282"
     CDS             1618..1788
                     /locus_tag="ACHLAM_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854760.1"
                     /translation="MQQFAIAGAASVRPFNPILSVQHSRKNILTGADFKQPRVKSLLD
                     RLVEFLNQKVQP"
     gene            1785..1949
                     /locus_tag="ACHLAM_003283"
     CDS             1785..1949
                     /locus_tag="ACHLAM_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854761.1"
                     /translation="MKKPTYEELEEALKELRRMAFARRTNSHNCGPFQYSDLCEDIIE
                     VTQLIKVVKQ"
     gene            2034..2342
                     /locus_tag="ACHLAM_003284"
     CDS             2034..2342
                     /locus_tag="ACHLAM_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854762.1"
                     /translation="MILTLNDKREISQIIASFTDEDYERINSEVDRLCKRCDPISEML
                     RLYKPDEHTKDAIDWLEDDDCNYQEKAAEWFWDAITERVKAEYAFAIFKRRHIYGEAA
                     "
     gene            2339..3241
                     /locus_tag="ACHLAM_003285"
     CDS             2339..3241
                     /locus_tag="ACHLAM_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO7854763.1"
                     /translation="MSNIVEFVKQQEQLFCGALTEQTVTWAKESQFAIQYFQKNDYLA
                     KTALANPTSAQNAIINVAAIGITLNPASKLAYLVPRDGMVCLDISYMGLLHLAQSTGS
                     IKWGQCKLVYSNDTYESNGLDSAPTHKYNAFGERGSIVGGYCTVKTADGDYLTEEMSL
                     AEIKAVEATSKAKNGPWKTFWEEMARKTIVKRASKYWPKAQRLDNAIHLLNEDEGMHQ
                     EPVMPHKSEEDIREDERKRQQEITDKAQLLCDEMAQAENMDDLKRYFAEAYRLTSGMK
                     LQQNIQAIYIECKAKLEVASEQTI"
     gene            3225..3707
                     /locus_tag="ACHLAM_003286"
     CDS             3225..3707
                     /locus_tag="ACHLAM_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFO7854764.1"
                     /translation="MSKLYEIANEYAKLMDSDLEPEMIADTIEGMEGEFTDKIEQLLA
                     IIKNESGYAERLKEEAKSLNERAAVIQNKIDSIMAYIASSLEMVGKKKIRAGIHQVTI
                     RKPSETVEIIDSSAIPPEYVEFETTIKADKLAIKHQLKAGINIPGAQLKVGKPSLLIK
                     "
     gene            3719..4033
                     /locus_tag="ACHLAM_003287"
     CDS             3719..4033
                     /locus_tag="ACHLAM_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854765.1"
                     /translation="MKKTPWEKWEVDFLREVAATMPVEVIAEKLERTEKAVMAKATRI
                     GADIVSRLRGRRWTRAEVSLFGKFSAEEIAIATCRSIYSVRAMRYKLKKLDEERAGIR
                     IN"
     gene            4050..4214
                     /locus_tag="ACHLAM_003288"
     CDS             4050..4214
                     /locus_tag="ACHLAM_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO7854766.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRSGKK"
     gene            4211..4411
                     /locus_tag="ACHLAM_003289"
                     /pseudo
     CDS             4211..4411
                     /locus_tag="ACHLAM_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4430..4780
                     /locus_tag="ACHLAM_003290"
     CDS             4430..4780
                     /locus_tag="ACHLAM_003290"
                     /inference="COORDINATES: protein motif:HMM:NF025303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854767.1"
                     /translation="MKSAIPESRDIEDDNDNMDDVSLAEDFGFNHAIERMRRQIPETP
                     TTDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVGKVEMTEW
                     LVDFAAQLRKGGSQ"
     gene            4777..5841
                     /locus_tag="ACHLAM_003291"
     CDS             4777..5841
                     /locus_tag="ACHLAM_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7854768.1"
                     /translation="MSKIDYQALRELAKQATQGEWVAFISTGTGTYAVHTPGDKRCED
                     VIKWTGFDGQKNAENNARYIAAFNPEVVQALLDENESMCRDLIARNGEIEGLRKLVAE
                     LEETKSKLNEQREYYEGVISDGSKRIAELEKSEEQLINERDHAESALADMYFAATGDR
                     PEWSNCFSFSDAVDAVVDRIADLEAKQPSPVVPDNTSESLAYAYKELTPEIMRGHIAV
                     FERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLNQLSGNSPVTPDGW
                     ISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLY
                     LKEVTHWMPLPEPPQEVNRG"
     gene            5834..6118
                     /locus_tag="ACHLAM_003292"
     CDS             5834..6118
                     /locus_tag="ACHLAM_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO7854769.1"
                     /translation="MANLQLAVNGEYFDQMKSGEKTEEYRLCNDYWNKRLVNRKYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            6191..6358
                     /locus_tag="ACHLAM_003293"
     CDS             6191..6358
                     /locus_tag="ACHLAM_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854770.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMLWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            6385..6729
                     /locus_tag="ACHLAM_003294"
     CDS             6385..6729
                     /locus_tag="ACHLAM_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854771.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            6853..7053
                     /gene="torI"
                     /locus_tag="ACHLAM_003295"
     CDS             6853..7053
                     /gene="torI"
                     /locus_tag="ACHLAM_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO7854772.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            7321..7503
                     /locus_tag="ACHLAM_003296"
     CDS             7321..7503
                     /locus_tag="ACHLAM_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7854773.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(7583..8830)
                     /locus_tag="ACHLAM_003297"
     CDS             complement(7583..8830)
                     /locus_tag="ACHLAM_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854774.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8902..9816)
                     /locus_tag="ACHLAM_003298"
     CDS             complement(8902..9816)
                     /locus_tag="ACHLAM_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO7854775.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            10032..11465
                     /gene="cscA"
                     /locus_tag="ACHLAM_003299"
     CDS             10032..11465
                     /gene="cscA"
                     /locus_tag="ACHLAM_003299"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO7854776.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(11473..12468)
                     /locus_tag="ACHLAM_003300"
     CDS             complement(11473..12468)
                     /locus_tag="ACHLAM_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7854777.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHISLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDREGGRVTRIPCPLLIRCST"
     gene            12711..12929
                     /locus_tag="ACHLAM_003301"
                     /pseudo
     CDS             12711..12929
                     /locus_tag="ACHLAM_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12947..14278
                     /gene="dsdA"
                     /locus_tag="ACHLAM_003302"
     CDS             12947..14278
                     /gene="dsdA"
                     /locus_tag="ACHLAM_003302"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO7854778.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(14383..15921)
                     /gene="emrY"
                     /locus_tag="ACHLAM_003303"
     CDS             complement(14383..15921)
                     /gene="emrY"
                     /locus_tag="ACHLAM_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO7854779.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15921..17084)
                     /gene="emrK"
                     /locus_tag="ACHLAM_003304"
     CDS             complement(15921..17084)
                     /gene="emrK"
                     /locus_tag="ACHLAM_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO7854780.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
BASE COUNT         5153 a         3897 c         4026 g         4218 t
ORIGIN      
        1 gtactattta gccatcagca ggacgcacta accaccattg aaggtgaggc tcttaaaaat
       61 ttagccctga agaagggcag cattcaaagc agaaagcttt ggggtgtggt gaagggttca
      121 tggatgggaa tatgtcgcac gtaaagcggc gaggcctgcg gaactattgc cgaattgaag
      181 tcggccgaag caggtcgaaa tgggtctccc acctaccaca ccaccaaagc taactgacag
      241 gagaatccag atggatgcac aagcacgccg ccgcgaacgt cgcgcagaga aacaggctca
      301 atggaaagca gcaaatcccc tgttggttgg ggtaagtgca aaaccagtta accgcccaat
      361 tctctcgctg aatcgcaaac cgaaatcacg agtagaaagc gcactgaatc cgatagacct
      421 tacagtgctg gctgaatacc acgaacagat tgaaagcaac ctgcaacgta ttgaacgcaa
      481 gaatcagcgc acatggtaca gcaagccacg cagtgaaatg ggtgtgacct gttcaggccg
      541 ccagaagcaa cgcggaaaat caattccagc ttattacgat tgaggtgaga tatggaagaa
      601 caagcaaaca agattctcgt agaactactg caaaaagcca gcaatggaat agacgcggct
      661 gtttcattca gccaagcgca gattcctgat gttgttcatc agttgctgct atggaatatg
      721 gttgacagtc tgattaaaac attaatagcc attctaacaa tcccactggt tttctggttt
      781 atgaagaagc agtgccaaag agttgagaca ggtaaaatcg gtgatgaagg atactcatgg
      841 gagaggggaa atcccaaata caggccgaca atggtttggg atagcaaagg agatattaac
      901 cttcttatca tgccattggt tggagttttg actctgtggg ggatttttat tattggtgta
      961 gtaaccaata tgacttggtt aaaaatttgg ctggccccaa agctttacct tatcgaatat
     1021 gcagcatcat tggttaagta atttcaggcc gcatagtcgg cctttatttt tggcataaac
     1081 aatatggggt aaaaatgaaa gttttaatgg tttatgaaaa tgttccagag tcgactgaaa
     1141 tctatatttt tgatgccaat gaagatgaag ttaacgattt gaaattgtct cacggcaatt
     1201 acacaaatgc taattgtgat gaaagtatcg aaaaagcact atcgcgtgtt cttgtcagaa
     1261 ttagtgatcc agaacattgt gatgatgatt ggctttctta ttgtggagcg gtaaaaactg
     1321 atgcaggaaa atggagtaaa agtaaagttg ataactcaac tccaatcatt atgaaagata
     1381 gtgatattga aatggtaata ataaccggaa tgattatgta ggctgagaat aagcactgtg
     1441 tattcattcc aacgagtgaa tacacggagc aatgtcgctc gtaaccaaac aggagccgac
     1501 ttgttctgat tattggaaat cttctttgcc ctccagtgtg agggcctttt tatatgcata
     1561 ccaataacgc ttcacttgag gcgttttcgt tatgcaatca aacagaagga gcatcctatg
     1621 caacagttcg ctattgcagg ggcggcatcg gttcgccctt tcaacccgat tttatcggta
     1681 cagcattcac gaaaaaacat tttaaccgga gcagacttta aacaacctcg cgttaagagc
     1741 ttgctggatc gtcttgttga gtttctgaat caaaaggtac agccatgaaa aaaccaactt
     1801 acgaagaact ggaagaagcc ctgaaagaac ttagaagaat ggctttcgcc cgacgcacta
     1861 actctcacaa ctgcggccca tttcagtact ctgatttatg cgaggacatc attgaggtaa
     1921 ctcaactgat taaggtcgtt aagcaatgag cattgcggat acatggtcag acgaagaatt
     1981 cattcgtcag atgaacaaaa tgctcaatca gcacaaagaa caggagaaag atgatgattc
     2041 tgactctgaa tgataagcgt gaaatatcgc aaataatcgc aagttttact gatgaagatt
     2101 acgagcgaat caacagtgaa gttgatcgcc tctgcaaacg ttgcgaccca ataagcgaaa
     2161 tgcttcgctt atataaacca gatgaacaca ctaaggacgc tatcgactgg ctggaagatg
     2221 atgactgtaa ctatcaggaa aaagccgctg aatggttctg ggatgcaata accgaaagag
     2281 ttaaggctga atatgccttc gcaatattca aacgcagaca tatttatgga gaagctgcat
     2341 gagcaatatc gttgaattcg ttaaacagca ggagcagtta ttctgcggag cattgactga
     2401 acagacggtg acatgggcta aggaaagcca gtttgcaatt cagtatttcc agaaaaacga
     2461 ttacctggct aaaacagcac tggcaaatcc aaccagcgca cagaacgcca tcatcaatgt
     2521 tgcggcgatc ggcatcacct taaacccggc cagcaaactg gcttatctgg ttcctcgcga
     2581 cggcatggtg tgccttgata tcagttacat gggattactt catcttgcgc aatcgacagg
     2641 atcaattaag tgggggcaat gcaaactggt gtactcaaac gacacctatg aatcaaatgg
     2701 ccttgattca gcaccaaccc acaaatacaa cgcatttggt gagcgaggct ctattgttgg
     2761 aggttattgc acggttaaaa cagcagatgg tgactacctg actgaagaaa tgagtctggc
     2821 agaaattaaa gctgtggaag caacgagcaa ggctaagaat ggaccgtgga agacattctg
     2881 ggaagagatg gcgcgtaaaa ccatagttaa acgcgccagc aaatactggc ctaaagccca
     2941 gcgactggat aatgccattc acctgcttaa cgaagatgaa ggtatgcatc aggaaccagt
     3001 tatgccgcac aaatcagagg aagatatccg cgaagatgaa cggaaacgcc agcaggaaat
     3061 aactgataaa gcacaacttc tttgtgatga aatggctcag gctgaaaaca tggatgattt
     3121 gaagagatat tttgcagaag catatcgcct gacatctgga atgaaattgc agcagaacat
     3181 acaagccatt tacatagaat gcaaagcgaa actggaggtt gccagtgagc aaactatatg
     3241 aaatagccaa tgaatacgca aaattgatgg attcagattt agagccagag atgattgctg
     3301 acacaataga aggcatggaa ggagaattta ccgataaaat agagcaactt ctcgccatta
     3361 ttaaaaatga atctggttat gctgaacgcc tcaaggaaga ggcaaagtca ctaaatgaac
     3421 gagcagcagt aattcaaaat aagattgata gcattatggc gtatatagcg tcatcgcttg
     3481 aaatggttgg caagaaaaag attagagcag gtattcacca ggtaacaatc cgcaaaccgt
     3541 cagaaactgt agaaatcatc gactcaagcg ccattcctcc tgaatacgtt gagttcgaaa
     3601 cgacaattaa agccgacaaa ctggcaatca aacaccaact aaaagcagga ataaatatcc
     3661 caggtgctca actcaaagtt gggaaacctt cacttcttat caaataacgg tatcgcctat
     3721 gaaaaagact ccatgggaga aatgggaagt cgatttcttg cgcgaggtag cggcgacaat
     3781 gccagttgaa gttatcgcag aaaaactgga aaggactgaa aaagcagtaa tggcgaaagc
     3841 aacaaggatt ggcgctgaca ttgttagccg acttcgtgga agacgctgga caagagccga
     3901 agtatcactt ttcggtaagt tctccgcaga agaaatagca attgcaacct gccgctcaat
     3961 ttattcagta agagctatgc gatacaagct aaaaaaactc gatgaagaaa gagcaggcat
     4021 acgaataaat taacaaagag gaatttacca tgagaggact tgcatacaat cccggcattc
     4081 ttccggcaga aatgattatt cgccaacgcg taaagccaat gccatcgaga gaggaattgc
     4141 ttaagagaaa ttcttttcca tcagtgaatc aaaacaaata tctgaatgcg atgtggcgga
     4201 gtgggaagaa atgaaacaaa tgtcactaat tgagatggat ggatttctga aaggtaaatg
     4261 catcccacga gatttaaagg ttaacgaaac aaacgctgaa tatcttgtcc gtaagttcgg
     4321 tgaacttgaa tcaaaactgg aaacggcgtt gcgggagtgt cgttctgctg gaatcacgat
     4381 tgataacctt gaggccaagt gcgcggcgct ggcggcggag aatgcgggaa taaagtctgc
     4441 aattccagaa tcacgggata ttgaggatga caatgacaat atggatgacg tatctctcgc
     4501 ggaagacttc gggttcaatc atgcaataga acggatgagg agacagatac ctgaaacgcc
     4561 aaccactgat gctttcctgg ctgaagtccg ggcgcagggg gtggatgctg ctatagaagc
     4621 tgcaaaaaat ctggtggccc aagaatatga gtataaggat ttcaaagcgg cgcagagtga
     4681 ttgctgtatg caccctggtt cagacctggt agggaaggtt gaaatgactg agtggttagt
     4741 tgactttgct gcccagcttc gcaaaggagg cagccagtga gcaagattga ttatcaggca
     4801 ctgcgtgagt tagcaaaaca ggcaacacaa ggagaatggg ttgcatttat ttcgacgggt
     4861 actggtacgt atgcggtgca tacgcccggt gataaacgat gtgaagacgt tatcaaatgg
     4921 accggctttg atggacagaa aaacgcagag aacaacgctc gttatatcgc agctttcaac
     4981 cctgaagtag tacaggcact gctggatgag aacgaaagca tgtgccgcga cctcattgct
     5041 cgcaatggtg agattgaagg gcttcgaaaa ctggttgctg agcttgaaga gacaaaatca
     5101 aaactcaacg agcagcgtga atattatgaa ggtgttatct cggatgggag taagcgtatt
     5161 gctgaactgg agaaaagcga agagcagctc attaacgagc gtgaccatgc tgagtctgct
     5221 ttagctgata tgtattttgc agcaaccggg gataggccgg agtggagtaa ctgtttcagt
     5281 ttttcagatg ccgtcgatgc cgtggttgac agaattgctg atttagaagc taaacagcca
     5341 tcgccagtag tgccggataa tacatcagag tctcttgctt atgcttacaa agagcttacg
     5401 cctgagatta tgcgcggtca tatcgctgta ttcgagcgat atggaatagc cccaaacgat
     5461 agctccacca caattcaggc actgcgaatc gcgctggatg gcatagagcg gagcgacgcc
     5521 atgcttcatg gtgccgaacc tgtaagccaa acttacaagt tgaaccagct atcgggcaac
     5581 tctccggtaa ctccggatgg ttggataagc tgtagtgagc gaatgccaga aatgggagag
     5641 cgacaatgct atgtgttagc ggctgacttt aaaaacaact acccaccaaa catccccaac
     5701 actcaggtcg gcgtatatgg cgactggttt aatgatggca atcccacttg ggatgacggt
     5761 gatggcgaag acctgtatct caaagaggta acccactgga tgccgctacc agaaccgccg
     5821 caggaggtta accgtggcta acctgcaact ggcagttaac ggtgaatatt tcgaccagat
     5881 gaagtcaggc gagaaaacgg aagagtatcg cctgtgtaat gactactgga ataagcgcct
     5941 cgttaaccgt aagtatgacc gcctgattat cacaaaggga tatccgaagc gcgacgattc
     6001 cagccgcaga attgacgtcc cgtatgacgg atatgaaatc aagacaatca cacatccgca
     6061 cttcggcgat aaaccggtaa aggtgttcgc gataaaggtg aatatcggca atgaataaca
     6121 atcctcgcat tcgcggggat ttcttttatc tgaactcgct acggcgagtt ttgttttatg
     6181 gagatgataa atgcacttcc gagtcacagg agaatggaat ggagagccat tcaacagagt
     6241 tatcgaagca gagaacatca acgactgcta tgaccactgg atgctatggg cacagatagc
     6301 acatgcagac gtaaccaata ttcgaattga agaactgaaa gaacaccaag ccgcctgatg
     6361 gcggtttttt attggagaca agaaatgtca gatttggcta tgaaggtttt gaaatggcaa
     6421 tcgactggcg atgtcggcat cagtagcgca actcttgcct caatcgcatg tggactgaaa
     6481 aagaatatct atggtcatag cttcggtgct ccacatgacg cagccgattt cagacgatgc
     6541 gttgcacttg ttgagcagat tccagaaatc agagattcat tcgacaaggt tgcaaagcgc
     6601 gttccggcat tcaaaggaat cctcaacgaa tgggattcac tcgttgctct gttgaagtct
     6661 gaaatgaaga cgtacgggaa caaagcacca gagacttaca gaagaatcag cgagctacgc
     6721 aaggactaac ccgcctcaca ctcgatgagg cattttcatt tatcaagata tccagaccta
     6781 ccatcgccgc atcaatacgg tttttttatt acctgatttg caggttcgat tccatattcg
     6841 gagatagcac tcatgcaaca cgaactacaa cctgattcac tggttgattt gaaattcatc
     6901 atggctgata ctggctttgg taaaaccttc atctatgacc ggattaagtc cggcgacctg
     6961 ccaaaagcca aagttatcca cgggcgagca agatggttat atcgtgacca ttgtgaattc
     7021 aaaaataagc tcttaagccg cgccaatggg taaaatagcg ggtaaaatat ttctcacatc
     7081 taaaaaacac cattccaatc aatcccctgt cgcttcaagt agatgtctgc aggggacacc
     7141 agataccctt caaaccaaat ctaccttcac cccgtaaaag atgggtttgg cagcacactt
     7201 gccctatatc tactcatttt tactgcaaca ggttgaaatc tcagcactgt cagaaagcgc
     7261 tgatgactaa acagccctgg gccgggcgat gtaaccatca tacagaatcc tgatagcgaa
     7321 atatggcgtg actcgatact tcactctgca atgcattcct tgatgaattc gcaggcccgt
     7381 gatacacggg acacgtcgct gaatgacgac aatgtcctgg aaatcagcga accgcgtatc
     7441 cggagtacat ttgagcgact gtaccagaac atgaatgagg cgtttggatt aggtgattat
     7501 tagcaggggt aagcattttt gtattattat tttccggttg agggatatgg agatatcgac
     7561 aacaaccgga aaaagtttac gtctatattg ctgaaggtac aggcgtttcc ataactattt
     7621 gctcgcgttt tttactcagg aagaaaatgc caaatagcaa catcaggcag acaatacccg
     7681 aaattgcgaa gaaaactgtc tggtagcctg cgtggtcaaa gagtatccca gtcggcgttg
     7741 aaagcagcac aatcccaagc gaactggcaa tttgaaaacc aatcagaaag atcgtcgacg
     7801 acaggcgctt atcaaagttt gccacgctgt atttgaagac ggatatgaca caaagtggaa
     7861 cctcaatggc atgtaacagc ttcactaatg aaataatcca ggggttaacg aacagcgcgc
     7921 aggaaaggat acgcaacgcc ataatcacaa caccgataag taatgcattt tttggcccta
     7981 cccgattcac aaagaaagga ataatcgcca tgcacagcgc ttcgagtacc acctggaatg
     8041 agttgagata accatacagg cgcgttccta catcgtgtga ttcgaataaa cctgcataaa
     8101 agacaggaaa gagttgttga tcaaaaatgt tatagaaaga ccacgtcccc acaataaata
     8161 tgacaaaaac ccagaagttt cgatccttga aaactgcgat aaaatcctct ttttttaccc
     8221 ctcccgcatc cgccgctatg cactggtgat ccttatcttt aaaacgcatg ttgatcatca
     8281 taaatacagc gccaaatagc gagaccaacc agaagttgat atggggactg atactaaaaa
     8341 atataccagc aaagaacgcg ccaatagcat agccaaaaga tccccaggcg cgcgctgttc
     8401 catattcgaa atgaaaattt cgcgccattt tttcggtgaa gctgtcaagc aaaccgcatc
     8461 ccgccagata ccccaggcca aaaaagagcg cccccagaat tagacctaca gaaaaattgc
     8521 tttgcagtaa cggttcataa acgtaaatca taaacggtcc ggtcaagacc agaatgaaac
     8581 tcatacacca gatgagcggt ttcttcagac cgagtttatc ctgaacgatg ccgtagaaca
     8641 tcataaatag aatgctggta aactggttga ccgaataaag tgtacctaat tccgtccctg
     8701 ttaatcctag atgtcctttc agccaaatag cgtataacga ccaccacagc gaccaggaaa
     8761 taaaaaagag aaatgagtaa ctggatgcaa aacgatagta cgcatttctg aatggaatat
     8821 tcagtgccat aattacctgc ctgtcgttaa aaaattcatg tcctatttag agataagagc
     8881 ggcctcgccg tttacttctc actattccag ttcttgtcga catggcagcg ctgtcatcgc
     8941 ccctttcgcc gttactgcaa gcgctccgca acgttgagcg agatcgataa ttcgtcgcat
     9001 ttctctttca tctgtagata atcccgtaga ggacagacct gtgagtaacc cggcaacgaa
     9061 cgcatctccc gcccccgtgc tatcgacaca attcacagac attccagcaa aatggtgaac
     9121 ttgtcctcga taacagacca ccaccccttc tgcaccttta gtcaccaaca gcatggcgat
     9181 ctcatactct tttgccaggg cgcatatatc ccaatcgttc tgtgtttttc cactgataag
     9241 tcgccattct tcttccgaga gcttgacgac atccgccagt tgtagcgcct gccgcaaaca
     9301 caagcggagc aaatgctcgt cttgccatag atcttcacga atattgggat cgaagctgac
     9361 aaaacctccg gcatgccgga tcgccgtcat cgcagtaaat gcgctggtac gcgaaggctc
     9421 ggcagacaac gcaattgaac agagatgtaa ccattcgcca tgtcgccagc agggcaagtc
     9481 tgtcgtctct aaaaaaagat cggcactggg gcggaccata aacgtaaatg aacgttctcc
     9541 ttgatcgttc agatcgacaa gcaccgtgga tgtccggtgc cattcatctt gcttcagata
     9601 cgtgatatcg acaccctcag ttagcagcgt tctttgcatt aacgcaccaa aaggatcatc
     9661 accgacccga cctataaacc cacttgttcc gcctaatctg gcgattccca ccgcaacgtt
     9721 agctggcgcg ccgccaggac aaggtagtag ccgcccgtct gattctggca agagatctac
     9781 gaccgcatcc cctaaaaccc atactttggc tgacattttt ttcccttaaa ttcatctgac
     9841 ttacgcatag tgataaacct ctttttcgca aaatcgtcat ggatttacta aaacatgcat
     9901 attcgatcac aaaacgtcat agttaacgtt aacatttgtg atattcatcg catttatgaa
     9961 agtaagggac tttattttta taaaagttaa cgttaacaat tcacccaatt tgcttaacca
    10021 ggatgataaa aatgacgcaa tctcgattac atgcggcgca aaacgcacta gcaaaacttc
    10081 acgagcgccg aggtaacact ttctatcccc attttcacct cgcgcctccc gccgggtgga
    10141 tgaacgatcc aaacggccta atctggttta acgatcgtta tcacgcgttt tatcaacatc
    10201 acccgatgag cgaacactgg gggccaatgc actggggaca tgccaccagc gacgatatga
    10261 tccactggca gcatgagcct attgcgctag cgccaggaga cgagaatgac aaagacgggt
    10321 gtttttcagg tagtgctgtc gatgacaatg gtgtcctctc acttatctac accggacacg
    10381 tctggctcga tggtgcaggt aatgacgatg caattcgcga agtacaatgt ctggctacca
    10441 gtcgggatgg tattcatttc gagaaacagg gtgtgatcct cactccacca gaaggcatca
    10501 tgcacttccg cgatcctaaa gtgtggcgtg aagccgacac atggtggatg gtagtcgggg
    10561 cgaaagatcc aggcaacacg gggcagatcc tgctttatcg cggcagttca ttgcgtgaat
    10621 ggactttcga tcgcgtactg gcccacgctg atgcgggtga aagctatatg tgggaatgtc
    10681 cggacttttt cagccttggc gatcagcatt atctgatgtt ttccccgcag ggaatgaatg
    10741 ccgagggata cagttaccga aatcgctttc aaagtggcgt aatacccgga atgtggtcgc
    10801 caggacgact ttttgcacaa tccgggcatt ttactgaact tgatagcggg catgactttt
    10861 atgcaccaca aagcttttta gcgaaggatg gtcggcgtat tgttatcggc tggatggata
    10921 tgtgggaatc gccaatgccc tcaaaacgtg aaggctgggc aggctgcatg acgctggcgc
    10981 gcgagctatc agagagcaat ggcaaacttc tacaacgccc ggtacacgaa gctgagtcgt
    11041 tacgccagca gcatcaatct atctctcccc gcacaatcag caataaatat gttttgcagg
    11101 aaaacgcgca agcagttgag attcagctgc agtgggcgtt gaagaacagt gatgccgaac
    11161 attacggatt acagctcggc actggaatgc ggctgtatat tgataaccaa tctgagcgac
    11221 ttgttttgtg gcggtattac ccacacgaga atttagatgg ctaccgtagt attcccctcc
    11281 cgcagggtga catgctcgcc ctaaggatat ttatcgatac atcatccgtg gaagtattta
    11341 ttaacgacgg ggaggcggtg atgagtagcc gaatatatcc gcagccagaa gaacgggaac
    11401 tgtcgctcta tgcctcccac ggagtggctg tgctgcaaca tggagcactc tggcaactgg
    11461 gttaacataa tatcaggtgg aacaacggat caacagcggg caagggatcc gcgtcactct
    11521 tcccccttca cgatcttcaa taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg
    11581 aagctgaatt gtggtcagcg gcggtaaaaa cagatgcccg acgccaacca gattatcaaa
    11641 gcccattacg gcgacatcct gcgggatacg tacccccttc gccaggagaa cctgataagc
    11701 gacaaaggct gcgcgatcgt taccacatat cagaacatca aaatctggtt tgcccgattt
    11761 gaagtggtca ttgagtaaac ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg
    11821 aaattgtttc acctcagcca gatctcgtcc agcatcacgc caggcctgct caaatccctg
    11881 ccgacgatac cctgttgcca acgcactttc cggtagccag aagcataacg gttgacgata
    11941 gcccgccgcg agcaaatgct gtgttgattc atattgtgca gtgtaatcat cagggatata
    12001 actgggtaac gctgggtcat ccgccacaca gttcgccaat acaatatttt caccatacag
    12061 agactcaggc agcgagatat gtcgcagccc cattgtagta tagataatgc catccggacg
    12121 gtgggcaagc agctgacgtg ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa
    12181 aaaactattc cagccaaact cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga
    12241 gaaaggagtg gtggccgtgt cctgcgccag cacggcgaga gtcgacggct tacgtccttg
    12301 agcgcgcatc ttacgggcgg aaagatcagg aacataattc agggtctgga ttgcctgcaa
    12361 tacgcggtca cgcgttgcag gacgcacaga ttctgcatta tgcatcaccc gggagactgt
    12421 catcatcgac actcccgcca ggcgtgcgac atcctttaat gaagccatac ccaagccgtt
    12481 tgccgtaaaa cgggcactgt agcagaaaca gacgtcactg gcgagatcca acgatattct
    12541 caacgcccta tcacccgaca cggcaataca ataaaaaata acaataattc ccggacaatt
    12601 gtacccaatt ccgcctctgt tctcgccaac gagtaaccat gtaggcaagt aggtaaaaca
    12661 tgccaactgg catcgccaac ctggagaaga aaatataaat atggatcatt gtgctgccgc
    12721 tgtatcccga catcagtccg gaaattattg cgattgctat cggttcaggt gcgattggct
    12781 gcacgatcgt tacggactcg cttttctggc tggtgaagca atattgcggc gctacgctca
    12841 atgaaacatt taaatactat acgacagcga catttatcgc ttcagtcgtc gctctggcgg
    12901 gcacattcct gctgtcattt atcatctaag cgcaaagaga cgtactatgg aaaacgctaa
    12961 aatgaactcg ctcatcgccc agtatccgtt ggtaaaggat ctggttgctc ttaaagaaac
    13021 cacctggttt aatcctggca cgacctcatt ggctgaaggt ttaccttatg ttggcctgac
    13081 cgaacaggat gttcaggacg cccatgcgcg cttatcccgt tttgcaccct atctggcaaa
    13141 agcatttcct gaaactgctg ctactggggg gattattgaa tcagaactgg ttgccattcc
    13201 ggctatgcaa aaacggctgg aaaaggaata tcaacaaccg atcagcgggc aactgctact
    13261 gaaaaaagat agccatttgc ccatttccgg ctccataaaa gcacgcggcg ggatttatga
    13321 agtcctggca catgcagaaa aactggctct ggaagcgggg ttgctgacgc ttgaagatga
    13381 ctacagcaaa ctgctttctc cggagtttaa acagttcttt agtcaataca gcattgctgt
    13441 gggctcaacc ggaaatctgg ggttatcaat cggcattatg agcgcccgca ttggctttaa
    13501 ggtgacagta catatgtctg ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca
    13561 tggcgttacg gtcgtggaat atgagcaaga ttatggtgtt gcagtcgagg aaggacgtaa
    13621 agcagcgcaa tctgacccga actgtttctt tattgatgac gagaattccc gcacgttgtt
    13681 ccttgggtat tccgtcgctg gccagcgtct taaagcgcaa tttgccgagc aaggtcgtat
    13741 tgtcgatgct gataaccctc tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg
    13801 tggcgtcgca ttcgggctta aactggcatt tggcgatcat gttcactgct tttttgccga
    13861 accaacgcac tccccttgta tgctgttagg cgtccataca ggattacacg atcagatttc
    13921 tgttcaggat attggtatcg ataaccttac cgcagcggat ggccttgcag ttggtcgcgc
    13981 atcaggcttt gtcgggcggg caatggagcg tttactggat ggcttctata cccttagcga
    14041 tcaaaccatg tatgacatgc ttggctggct gacgcaggaa gaaggtattc gtcttgaacc
    14101 ttcggcactg gcgggtatgg ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca
    14161 gatgcacggt ttcagcgcag aacaactgca taatgccact catctggtgt gggcgacggg
    14221 aggtggaatg gtgccggaag aagagatgga gcaatatctg gcaaaaggcc gttcataacg
    14281 tttcaacgca gcatcgccat cctttccttg ggtgagcgat gctgccgatg gcgcagactt
    14341 aagatccccg gtcttacctg ctataattct ctctttatac catcacccaa cgcctttcgc
    14401 tgtaaacggc ggtttcgcaa accaaaccaa taccgtaagt aagataaaag caatagccgc
    14461 catacggaaa atctcatttg cagaaattga aagtgactgc tgagttattt cattattaat
    14521 ttcattaaga actcctgaaa gcgaaccata gtatttatcc ataatttgtg acgatgaatt
    14581 aaatacgggg ttaaattgat cgatggttgc tgtcaactga ctatggtgta acgattcgcg
    14641 tcgtccccac agcgtcattg tcaacgacgt accaactgat cctgacaagg tacgaaaaaa
    14701 attactcata ctcgaggcat tggcaaattt attatctggc aaccctgaaa acgaaatcgt
    14761 tgttaagggt aaaaagaaac aggcaacggc gaatccctgg aaaaactgtg gcaaaatgat
    14821 gcctgtaaaa tcaatcgttg gcataaatgt cacagaacgc cagtaatagc aaaccgcata
    14881 catcaaaaaa ctaaatgtca ccaacaaccg catatcaatt ttgttgccat aacgtcctat
    14941 caaaggtgat attaatagcg gcatgatgcc gatgggcgca taggcaagtc cggcccatat
    15001 cgcattatac cccatcgttt cctggagtaa ctgcggcata aggactatcg ccccagagta
    15061 aaataaatac gcgcatgtga tactcacaat accaatggtg aaattacggg acttaaacaa
    15121 actgagatca agaatcggat tctctgaggt cgactcccaa atgactaaag aaatcagaga
    15181 aataactgac actactgtta gtattattat tgtactcgag ttgaaccaat ccagatcgcg
    15241 ccctttatca agcattatct gcaagccacc aacaccgagc actaacaggg tcagtcctgg
    15301 tagattcatt ttgaccggtg aagtctcagt ttctcttcct ttaagtaagg ttaagcataa
    15361 tgtcagaacg ataatcccca tagggacatt gattaaaaat atccaacccc agctaaagtt
    15421 atcgcaaata tagccgccca atatcggccc acatatcgga gcgataatta cggtcattga
    15481 ccataatgcc aaagcaaatg ttcttttttc tggcggataa ttccttaata ataaactctg
    15541 tgacagtgga attaacggcc ccgccattaa gccctgaacg actctaaaaa atatcagcac
    15601 atcaagattg gtcgataggc tacacattaa tgaagatagt gaaaaaaagg ttactgaaag
    15661 taaaaataat cttaattcgc ctattctttg tgccagcctg ccggtaacag ggatcgcaat
    15721 ggcatttgct acgccaaacg aagtgataac ccaggtgcct tcgtctgttg atgctcccag
    15781 aaagccagat attgtcggta ttgcgacgtt agaaatagtg gaatccaaca tttgcataaa
    15841 cgtcgctaag gacaatgcaa tagtaacgca ccataacgtc ccaccggtta atggtgccgg
    15901 agttgattta gtgattgcca ttaaagttgt ccattatgcg aaataatgtt gctaatttct
    15961 ttttctatcg gagtagtatc gataactaaa gccttactgg tataagcagg catggaggtc
    16021 acggttgaag ccagctcagg catctcagca atgtcttcgt ccttcgtatc aatagttgca
    16081 gtcatcgata aaccaatacg taagggatgt tccatgagtt cttttggatc aagagaaact
    16141 tcaaccggta cacgctgaac gattttgatc cagttccctg tcgcattttg tgcaggtaat
    16201 aaggagaacg cattgccggt tcccatattg attcctgtca cccgaccatg aaacacaaca
    16261 ttttcaccat aaagatcgct gataatattg accgattgac caatccgtac atccgtgagt
    16321 tgtgtttctt taaagttggc attaacccac atttgacgtg ccggtacgac agccattaac
    16381 gattgtccgg gactcactgt ttcgccgacc tgaacacttc gctgggcaat atagccggta
    16441 acaggactct taatatccgt acgtttaagc gctaaccagg cttctttggt tgcatctgcc
    16501 gcttcaacga cttgtggctg acggtttaat ggtgtgttca ttactaaagc tttattcgct
    16561 ttataagcct ggatagcggc attcaatgcc gctttgctac ttattaacgt atctttggta
    16621 tgctccagcg tttcttttga aataaccccc tgcttcgcta aaggcacacg acggttataa
    16681 tcttctaaag attgttgata ctgaatacgt gctgaagcga cttcggcact gtattgttta
    16741 tcctgtaagt acagtttatt cgtttgccga acaatatttg ccagattatt tttagcttta
    16801 ttgagtgcga tagtggcatc agtcttatcc agtgagacta aaatgtcacc ctgtcgaacg
    16861 tagttcgtat ctttatgatt aacgacagtg acgctgcctg aaacttgtgc agaaattgga
    16921 tctgcattcc ccgtgacata ggcgtcatct gtactaatca tgtcttctaa ttccattgac
    16981 caataggcat aggcacctga aaacgcaata aataaaacta ccgccaataa agaaaagtat
    17041 tttcttctgt tagaatgttt tttatttgaa ttaatctgtt ccacttttat ctctcatttc
    17101 gcatagatga aatttatgaa ttgaatgaaa agagtattca ttcttcatat caaacacagt
    17161 gcatgtgttg cacattatcc caggaacgtt ggaggcctaa tttaaatgag gtgcaaaaaa
    17221 aacaacagag gactaaaccg tggcttttgc aatacaattc ttacgcatgt aggattagtg
    17281 agaagactta tagt
//
LOCUS       DAZGAP010000115        17268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGAP010000115 DAZGAP010000000
VERSION     DAZGAP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.116"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            261..665
                     /gene="sdhC"
                     /locus_tag="ACHLAM_003305"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             261..665
                     /gene="sdhC"
                     /locus_tag="ACHLAM_003305"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7854781.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            659..1006
                     /gene="sdhD"
                     /locus_tag="ACHLAM_003306"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             659..1006
                     /gene="sdhD"
                     /locus_tag="ACHLAM_003306"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO7854782.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1006..2772
                     /gene="sdhA"
                     /locus_tag="ACHLAM_003307"
     CDS             1006..2772
                     /gene="sdhA"
                     /locus_tag="ACHLAM_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO7854783.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASASDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2788..3504
                     /gene="sdhB"
                     /locus_tag="ACHLAM_003308"
     CDS             2788..3504
                     /gene="sdhB"
                     /locus_tag="ACHLAM_003308"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO7854784.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3805..6606
                     /gene="sucA"
                     /locus_tag="ACHLAM_003309"
     CDS             3805..6606
                     /gene="sucA"
                     /locus_tag="ACHLAM_003309"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO7854785.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6621..7838
                     /gene="odhB"
                     /locus_tag="ACHLAM_003310"
     CDS             6621..7838
                     /gene="odhB"
                     /locus_tag="ACHLAM_003310"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO7854786.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPVKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7932..9098
                     /gene="sucC"
                     /locus_tag="ACHLAM_003311"
     CDS             7932..9098
                     /gene="sucC"
                     /locus_tag="ACHLAM_003311"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO7854787.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9098..9967
                     /gene="sucD"
                     /locus_tag="ACHLAM_003312"
     CDS             9098..9967
                     /gene="sucD"
                     /locus_tag="ACHLAM_003312"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO7854788.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10071..10793)
                     /gene="mngR"
                     /locus_tag="ACHLAM_003313"
     CDS             complement(10071..10793)
                     /gene="mngR"
                     /locus_tag="ACHLAM_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO7854789.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10963..12879
                     /gene="mngA"
                     /locus_tag="ACHLAM_003314"
     CDS             10963..12879
                     /gene="mngA"
                     /locus_tag="ACHLAM_003314"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO7854790.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALNLKRSDETRTVQQDT
                     QPVKSLKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            12897..15530
                     /gene="mngB"
                     /locus_tag="ACHLAM_003315"
     CDS             12897..15530
                     /gene="mngB"
                     /locus_tag="ACHLAM_003315"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO7854791.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLTFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKLWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREYEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(15675..17072)
                     /locus_tag="ACHLAM_003316"
     CDS             complement(15675..17072)
                     /locus_tag="ACHLAM_003316"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFO7854792.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSEGLWIRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALQATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRHWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
BASE COUNT         4119 a         4359 c         4755 g         4035 t
ORIGIN      
        1 aaactatatg taggttaatt gtaatgattt tgtgaacggc ctatactgcc gccaggtctc
       61 cggaacaccc tgcaatcccg agccacccag cgttgtaacg tgtcgttttc gcatctggaa
      121 gcagtgtttt gcatgacgcg cagttataga aaggacgctg tctgacccgc aagcagaccg
      181 gaggaaggaa atcccgacgt ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc
      241 cagggaataa taagaacagc atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa
      301 gacctgttaa tctggaccta cagaccatcc ggttccccat cacggcgata gcgtccattc
      361 tccatcgcgt ttccggtgtg atcacctttg ttgcagtggg catcctgctg tggcttctgg
      421 gtaccagcct ctcttcccct gaaggtttcg agcaagcttc cgcgattatg ggcagcttct
      481 tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta
      541 ttcgccacat gatgatggat tttggctatc tggaagaaac attcgaagcg ggtaaacgct
      601 ccgccaaaat atcctttgtt attactgtcg tgctttcact tctcgcagga gtcctcgtat
      661 ggtaagcaac gcctccgcat taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc
      721 taccgctatc gtcctgacgc tctacatcat ttatatggtc ggttttttcg ctaccagtgg
      781 cgagctgaca tatgaagtct ggatcggttt cttcgcctct gcgttcacca aagtgttcac
      841 cctgctggcg ctgttttcta tcttgatcca tgcctggatc ggcatgtggc aggtgttgac
      901 cgactacgtt aaaccgctgg ccttgcgcct gatgctgcaa ctggtgattg tcgttgctct
      961 ggtggtttac gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc
     1021 agagaatttg atgcagttgt aattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa
     1081 atttcccaga gcggtcagac ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat
     1141 accgtttctg cgcaaggcgg cattaccgtt gcgctgggta atacccatga agataactgg
     1201 gaatggcata tgtacgacac cgtaaaaggt tcggactata tcggtgacca ggacgcgatt
     1261 gaatatatgt gtaaaaccgg gccggaagcg attctggaac tggaacatat gggcctgccg
     1321 ttctcgcgtc ttgatgatgg tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac
     1381 ttcggcggcg agcaggcggc acgtactgcg gcggcagctg accgtaccgg tcacgcactg
     1441 ttgcacaccc tttatcaaca gaacctgaaa aaccacacca ccattttctc cgagtggtat
     1501 gcgctggatc tggtgaaaaa ccaggatggc gcggtggtcg gttgtaccgc actgtgcatc
     1561 gaaactggtg aagtggttta cttcaaagct cgcgctaccg tgctggcgac tggcggggca
     1621 gggcgtattt atcagtccac taccaacgcc cacattaaca ctggcgacgg cgtcggcatg
     1681 gctatccgtg caggcgtacc agtgcaggat atggaaatgt ggcagttcca cccgactggt
     1741 attgccggtg cgggcgtact ggtcaccgaa gggtgccgtg gtgaaggcgg ttatctgctg
     1801 aacaaacatg gcgaacgctt tatggagcgt tatgcaccga acgccaaaga cctggcgggc
     1861 cgtgacgtgg tggcgcgttc catcatgatt gaaatccgtg aaggtcgcgg ctgtgatggt
     1921 ccgtgggggc cacacgcgaa actgaaactt gaccatctgg gtaaagaagt tcttgaatcc
     1981 cgtctgccgg gtatccttga actctcccgc accttcgctc acgttgatcc ggtgaaagag
     2041 ccgattccgg ttatcccaac ctgtcactac atgatgggcg gtattccgac caaagtgacc
     2101 ggtcaggcgc tgacggtgaa cgagaaaggc gaagatgtgg ttgttccggg gctatttgcc
     2161 gttggtgaaa tcgcttgtgt atcggtacat ggcgctaacc gtctgggcgg caactcgctg
     2221 ctggacctgg tcgtatttgg tcgtgcggca ggtctgcatc tgcaagagtc tatcgccgag
     2281 cagggcgcac tgcgcgatgc cagcgcgtct gatgtagaag cgtctctgga tcgcctgaac
     2341 cgctggaaca ataaccgtaa cggtgaagat ccggtggcga tccgtaaagc actgcaagaa
     2401 tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag
     2461 cagttgaaag ttatccgcga gcgtctgaaa aatgcccgtc tggatgacac ttcaagtgag
     2521 ttcaataccc agcgcgttga gtgcctggaa ctggataacc tgatggaaac ggcgtatgca
     2581 acggctgttt ctgccaactt ccgtaccgaa agccgtggcg cacatagccg cttcgacttc
     2641 ccggatcgtg atgatgaaaa ctggctgtgc cactccctgt atctgcctga gtcggaatcc
     2701 atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc cggcattccc gccgaagatt
     2761 cgtacttact aatgcggaga caggaaaatg agactcgagt tttcaattta tcgctataac
     2821 ccggatgttg atgatgctcc gcgtatgcag gattacaccc tggaagcgga agaaggtcgt
     2881 gacatgatgc tgctggatgc gcttatccag ctgaaagaga aagatcccag cctgtcgttc
     2941 cgtcgctcct gccgtgaagg tgtgtgtggt tccgacggtc tgaacatgaa cggcaagaat
     3001 ggtctggcct gtattacccc gatttcggca ctcaaccagc cgggcaagaa gattgtgatt
     3061 cgcccgctgc caggtttacc ggtgatccgc gatttggtgg tagacatggg acaattctat
     3121 gcgcaatatg agaaaattaa gccttacctg ttgaataatg gacaaaatcc gccagctcgc
     3181 gagcatttac agatgccaga gcagcgcgaa aaactcgacg ggttgtatga atgtattctc
     3241 tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga atcccgataa gtttatcggc
     3301 ccggcaggct tgttagcggc atatcgtttc ctgatcgata gccgtgatac cgagactgac
     3361 agccgcctcg acggtttgag cgatgcattc agtgtattcc gctgtcacag catcatgaac
     3421 tgcgtcagtg tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg
     3481 atgttgttgc aacgtaatgc gtaaaccgta ggcctgagaa gacgcgcaag cgtcgcatca
     3541 ggcaaccagt gccggatgcg gcgtgaacgc cttatccggc ctacaagtca ttacccgtag
     3601 gcctgataag cgcagcgcat caggcgtaac aaagaaatgc aggaaatctt taaaaactgc
     3661 ccctgacact aagacagttt ttaaaggttc cttcgcgagc cactacgtag acaagagctc
     3721 gcaagtgaac cccggcacgc acatcactgt gcgtggtagt atccacggcg aagtaagcat
     3781 aaaaaagatg cttaagggat cacgatgcag aacagcgctt tgaaagcctg gttggactct
     3841 tcttacctct ctggcgcaaa ccagagctgg atagaacagc tctatgaaga cttcttaacc
     3901 gatcctgact cggttgacgc taactggcgt tcgacgttcc agcagttacc tggtacggga
     3961 gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac
     4021 gcttcacgtt actcttcaac gatctccgac cctgacacca atgtgaagca ggttaaagtc
     4081 ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg
     4141 ggactgtggc agcaagataa agtggccgat ctggatccgt ctttccacga tctgaccgaa
     4201 gcagacttcc aggagacctt caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa
     4261 ctcggcgagc tgctggaagc cctcaagcaa acctactgcg gcccgattgg tgccgagtat
     4321 atgcacatta ccagcactga agaaaaacgc tggatccaac agcgtatcga gtctggtcgc
     4381 gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc
     4441 cttgaacgtt acctcggcgc aaaattccct ggcgcaaaac gcttctcgct ggaaggtggt
     4501 gacgcgttaa tcccgatgct taaagagatg atccgccacg ctggcaacag cggcacccgc
     4561 gaagtggttc tcgggatggc gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt
     4621 aaaaaaccgc aagacttgtt cgacgagttt gccggtaaac ataaagaaca cctcggcacg
     4681 ggcgacgtga aataccacat gggcttctcg tctgacttcc agaccgatgg cggcctggtg
     4741 cacctggcgc tggcgtttaa cccgtctcac cttgagattg tcagcccggt cgttatcggt
     4801 tctgttcgtg cccgtctgga cagacttgat gagccgagca gcaacaaagt gctgccaatc
     4861 accattcatg gtgacgccgc agtgaccggg cagggcgtgg ttcaggaaac cctgaacatg
     4921 tcgaaagcgc gtggttatga agttggcggt acggtacgta tcgttatcaa caaccaggtt
     4981 ggcttcacca cctctaaccc gctggatgcc cgttcgacgc cgtactgtac tgatatcggc
     5041 aagatggtac aggcaccgat tttccacgtt aatgcggatg atccggaagc cgttgccttt
     5101 gtgacccgtc tggcgctcga tttccgtaac acctttaaac gtgatgtctt catcgatctg
     5161 gtgtgctacc gccgtcacgg ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg
     5221 atgtatcaga aaatcaaaaa acatccgacg ccgcgcaaaa tctatgctga caagctggag
     5281 caggaaaaag tcgcgacgct ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg
     5341 ctggatgctg gcgattgcgt tgtagcagag tggcgtccga tgaacatgca ctctttcacc
     5401 tggtcgccgt acctcaacca cgaatgggac gaagagtacc cgaacaaagt tgagatgaag
     5461 cgcctgcagg aactggctaa acgcatcagc acggtgccgg aagcagttga aatgcagtct
     5521 cgcgttgcca agatttatgg cgatcgccag gcaatggcag ccggtgagaa actgttcgac
     5581 tggggcggtg cggaaaacct cgcttacgcc acgctggttg atgaaggcat tccggttcgc
     5641 ctgtcgggtg aagactccgg tcgcggtacc ttcttccacc gccacgcggt gatccacaac
     5701 cagtctaacg gttccactta cacgccgctg caacacatcc ataacggtca gggcgcgttc
     5761 cgtgtctggg actccgtact gtcagaagaa gccgtgctgg cgtttgaata cggttatgcc
     5821 accgcagaac cacgcactct gaccatctgg gaagcgcagt tcggtgactt cgccaacggt
     5881 gcgcaggtgg ttatcgacca gttcatctcc tctggcgaac agaaatgggg ccggatgtgt
     5941 ggcctggtga tgttgctgcc gcacggttac gaagggcagg ggccggagca ctcctccgcg
     6001 cgtctggaac gttatctgca actttgcgct gagcaaaaca tgcaggtttg cgtaccgtct
     6061 accccggcac aggtttacca catgctgcgt cgtcaggcgt tgcgcgggat gcgtcgtcca
     6121 ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc tggcggtatc cagcctcgaa
     6181 gaactggcga acggcacctt cctgccagcc atcggtgaaa tcgacgagct tgatccgaag
     6241 ggcgtgaagc gcgtagtgat gtgttctggt aaggtttatt acgacctgct ggaacaacgt
     6301 cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg
     6361 cataaagcga tgcaggaagt gttgcagcag tttgctcacg tcaaggattt tgtctggtgc
     6421 caggaagagc cgctcaacca gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg
     6481 attccgtttg gggcttctct gcgttatgca ggccgcccgg cctccgcctc tccggcggta
     6541 gggtatatgt ccgttcacca gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc
     6601 gaataaataa aggatacaca atgagtagcg tagatattct ggtccctgac ctgcctgaat
     6661 ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg
     6721 atgaagtgct ggtagaaatc gaaactgaca aagtggtact ggaagtaccg gcatcagcag
     6781 acggcattct ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct cgtcagatcc
     6841 ttggtcgcct gcgtgaaggc aacagcgcgg gtaaagaaac cagcgccaaa tctgaagaga
     6901 aagcgtccac tccggcgcaa cgccagcagg cgtctctgga agagcaaaac aacgatgcgt
     6961 taagcccggc gatccgtcgc ctgctggctg aacataacct cgacgccagc gccattaaag
     7021 gcaccggtgt gggtggtcgt ctgacccgtg aagatgtgga aaaacatctg gcgaaagccc
     7081 cggtgaaaga gtctgctccg gcagcgcctg ctccggcggc gcaaccggcc ctggctgcac
     7141 gtagcgaaaa acgtgtgccg atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg
     7201 aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga agtcaacatg aagccgatta
     7261 tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct
     7321 ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa acgttacccg gaagtgaatg
     7381 cgtctatcga cggtgatgat gtggtctacc acaactattt cgacgtcagt atggcggttt
     7441 ctacgccgcg cggcctggtg acgccggttc tgcgtgatgt cgataccctc ggcatggcag
     7501 acatcgagaa gaaaatcaaa gagctggctg ttaaaggccg tgacggcaag ctgacggttg
     7561 aagatctgac tggtggtaac ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt
     7621 caacgcctat catcaaccca ccgcagagcg caattctggg tatgcacgct atcaaagatc
     7681 gtccgatggc ggtgaatggt caggttgaga tcctgccgat gatgtacctg gcgctgtcct
     7741 acgatcaccg tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt
     7801 tgctggaaga tccgacgcgt ctgctgctgg acgtgtagta gtttaagctt cacctgcact
     7861 gtagaccgac gtggataggg tgtcggtcta cggtttaaaa gataacgatt actgaaggat
     7921 ggacagaaca catgaactta catgaatatc aggcaaaaca actttttgcc cgctatggct
     7981 taccagcacc ggtgggttat gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa
     8041 aaatcggtgc cggtccgtgg gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag
     8101 cgggcggtgt gaaagttgta aacagcaaag aagacatccg tgcttttgca gaaaactggc
     8161 tgggcaagcg tctggtaacg tatcaaacag atgccaatgg ccaaccggtt aaccagattc
     8221 tggttgaagc agcgaccgat atcgctaaag agctgtatct cggtgccgtt gttgaccgta
     8281 gttcccgtcg tgtggtcttt atggcctcca ccgaaggcgg cgtggaaatc gaaaaagtgg
     8341 cggaagaaac tccgcacctg atccacaaag ttgcgcttga tccgctgact ggcccgatgc
     8401 cgtatcaggg acgcgagctg gcgttcaaac tgggtctgga aggtaaactg gttcagcagt
     8461 tcaccaaaat cttcatgggc ctggcgacca ttttcctgga gcgcgacctg gcgctgatcg
     8521 aaatcaaccc gctggtcatt accaaacagg gcgatctgat ttgcctcgac ggcaaactgg
     8581 gcgctgatgg caacgcactg ttccgccagc ctgatctgcg cgaaatgcgt gaccagtcgc
     8641 aggaagatcc gcgtgaagca caggctgcac agtgggaact gaactacgtt gcgctggacg
     8701 gtaacatcgg ttgtatggtt aacggcgcag gcctggcgat gggcaccatg gacatcgtta
     8761 aactgcacgg cggcgaacca gctaacttcc tggacgttgg cggcggcgca accaaagaac
     8821 gtgtaaccga agcgttcaaa atcatcctct ctgacgacaa agtgaaagcc gttctggtta
     8881 acatcttcgg cggtatcgtt cgttgcgacc tgatcgctga cggtatcatc ggcgcggtag
     8941 cggaagtggg tgttaacgta ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg
     9001 gcgcgaagaa actggctgac agcggcctga atattattgc agcaaaaggt ctgacggatg
     9061 cagctcagca ggttgttgcc gcagtggagg ggaaataatg tccattttaa ttgataaaaa
     9121 caccaaggtt atctgccagg gctttaccgg tagccagggg actttccact ctgaacaagc
     9181 cattgcatac ggcactaaaa tggttggcgg cgtaacccca ggtaaaggcg gcaccaccca
     9241 cctcggcctg ccggtgttca acaccgtgcg tgaagctgtt gctgctactg gcgctaccgc
     9301 ttctgttatc tacgtaccag caccgttctg caaagactcc attctggaag ctatcgatgc
     9361 aggcatcaaa ctgattatca ccatcactga aggcatcccg acgctggata tgctgaccgt
     9421 gaaagtgaag ctggatgaag caggcgttcg tatgatcggc ccgaactgcc caggcgttat
     9481 cactccgggt gaatgcaaaa tcggtatcca gcctggtcac attcacaaac cgggtaaagt
     9541 gggtatcgtt tcccgttccg gtacgctgac ctatgaagcg gttaaacaga ccacggatta
     9601 cggtttcggt cagtcgacct gtgttggtat cggcggcgat ccgatccctg gctctaactt
     9661 tatcgacatc ctcgaaatgt tcgaaaaaga tccgcagacc gaagcgatcg tgatgatcgg
     9721 tgagatcggc ggtagcgctg aagaagaagc agcggcgtac atcaaagagc acgttaccaa
     9781 gccagttgta ggttacatcg ctggtgtgac tgcgccgaaa ggcaaacgta tgggccacgc
     9841 gggtgcgatc attgccggtg ggaaagggac tgcggatgag aaattcgctg ctctggaagc
     9901 cgcaggcgtg aaaaccgttc gcagcctggc ggatatcggt gaagcactga aaactgttct
     9961 gaaataaata tctgtaataa gaaatagccc tcgccgcttc cttctacagg aatggcgaag
    10021 ggctgtcggt ttcgacatgg ttggccatcg tatgatggcc ttttttatgc ttatcgcgat
    10081 gattttcgct gcgctatcag ggtaaattta tagtcatcgg tattaaaagc gttgcggcta
    10141 tattcaaaca cccggccatc aactaaatat ccacgcgata ctttttcaag aatcggctta
    10201 gtctggctga tattaagcag acggctcatt tcttcggttg gcatcagagg aatgatttcc
    10261 tgttcgctac gatcgataac cattttcttc acttcttcaa taaagtgata tttcgaattt
    10321 tccatgacct gccaggtgag atccgggaac aacgcaagcg gcatccaggt ttcttccagc
    10381 gccattggct tttgcttgcg atagcgcacg cgcttcacat gccacacacg atcctgcgcg
    10441 gtgatttgta gctgttgctg aagaaaatcg tcagccggaa tcacttcgaa tatcagaact
    10501 tcactgtgtg tatcgacgtg acggtccgtc agtttttcat caaaactggt taactgaaaa
    10561 atatcgtaat tgacccgctc ttcttttacg taagtcccgc tgccctgaat actttcgagg
    10621 atctgctgtt caactaactg gcgcaaagcc tgacgcaccg taacccggct gacgccaaac
    10681 tctgtttgta gcgctgattc agtgggtaac gcatcgccag gtttaagctc gccacgcgca
    10741 atttgctcac gaatgcgatc agcaatctgt cggtataagg gcttccgtcc catgatttag
    10801 catctcatta atacgaattt agccattatg cccgataaat tcatcctgta aataatacaa
    10861 atacaataca aataatttca atcaagtgaa attgatcaca taatggtatt gttttatcgg
    10921 gcacactggc gcgactataa aaacgatcaa gtgaggatca tgatgaacct gacgactctg
    10981 acccaccgcg atgcgttgtg tctgaatgcg cgctttacca gccgtgaaga ggccatccac
    11041 gcgttgactc aacgtcttgc tgctctgggg aaaatttcca gtactgagca atttctgaaa
    11101 gaagtgtatc gccgtgaaag ccttggcccg accgccttag gtgaagggct ggctgtgccg
    11161 catggcaaaa ctgctgcggt aaaagaagcg gcgtttgcgg tcgccacact cagcgagccg
    11221 cttcagtggg aaggcgtgga tggcccggaa gcggttgatt tagtggtgtt gctggcgatt
    11281 ccccccaatg aagcgggtac tacgcacatg caactgctga cagcgctgac cacgcgcctg
    11341 gcggatgatg agatacgggc gcgtatacag tcggcgacga cgcctgatga attgctctcg
    11401 gcgctggatg acaagggagg cacgcaaccc tcagcttctt tttccaacgc gccaactatc
    11461 gtctgcgtaa cggcctgccc ggcgggtatt gctcacacct atatggctgc ggagtatctg
    11521 gaaaaagccg gacgcaaact cggcgtgaat gtctacgttg aaaagcaagg tgctaacggc
    11581 attgaagggc gtttaacggc agagcaactc aatagtgcaa ccgcctgtat ttttgcggct
    11641 gaagtcgcca tcaaggagag tgagcgtttt aacggcattc ccgcgctttc tgtgcctgtt
    11701 gccgagccga ttcgccatgc agaagcgttg atccaacaag cgcttaacct caagcgtagc
    11761 gatgagacgc gtaccgtaca gcaagatacg caaccggtga aaagcctcaa aacggagcta
    11821 aaacaggcac tgttgagcgg gatctctttt gcagtgccgt tgattgtcgc ggggggcacg
    11881 gtgctggcgg ttgcggtatt actgtcgcaa atcttcgggc tacaagattt gtttaatgaa
    11941 gaaaactcct ggctgtggat gtaccgcaaa ctgggcggag ggctgctcgg gattttgatg
    12001 gtaccggtgc tggcagccta caccgcctat tcactggctg ataaaccggc gttagcgcca
    12061 ggctttgcgg ctggacttgc cgccaacatg atcggttccg ggtttctcgg tgctgtcgtt
    12121 ggcggattga tagccggtta tctgatgcgc tgggtgaaaa atcacttgcg tcttagcagt
    12181 aaattcaatg gattcctgac tttttatctc tacccggtgc tcggtacgtt gggagcgggc
    12241 agtctgatgc tgtttgtggt gggggaacct gtcgcctgga tcaataactc gcttaccgcc
    12301 tggctgaacg gtctgtcagg aagtaacgcg ctgttgctgg gtgccattct cggttttatg
    12361 tgttcctttg accttggagg gccagtgaac aaagccgctt atgcattctg cctgggcgca
    12421 atggcgaacg gcgtttacgg cccgtatgcc attttcgcct ccgtcaaaat ggtttcggca
    12481 tttaccgtaa ccgcttccac gatgctcgca ccgcgcctgt ttaaagagtt tgaaattgag
    12541 accgggaaat ccacctggct gttagggctg gcaggtatta ccgaaggggc gatcccgatg
    12601 gcgattgaag atccgctgcg ggttattggt tcgtttgtgc tgggctctat ggtaacgggc
    12661 gctatcgtcg gcgcgatgaa tatcggcctt tcgacacccg gtgccggcat tttctcgctc
    12721 tttttacttc atgataatgg cgcgggcggt gttatggcgg caattggctg gtttggtgcg
    12781 gcattggtgg gggctgcaat ctcgactgca attcttctga tctggcggcg tcacgcggtt
    12841 aagcatggca actatctgac tgatggcgta atgccataaa caaaaaggaa acgacgatga
    12901 aagcggtatc tcgcgttcac atcaccccgc atatgcactg ggatcgggag tggtatttca
    12961 ccaccgaaga gtcacgcatt ctgctggtca ataatatgga agagatcctg tgccgactgg
    13021 aacaggacaa cgaatacaaa tattacgtac tcgacgggca aacggcgatc ctcgaagatt
    13081 atttcgcggt gaaaccggaa aacaaagacc gtgtgaagaa acaggtagaa gcaggcaagt
    13141 tgattatcgg cccctggtat acccagaccg ataccacgat tgtttctgcg gaatccatcg
    13201 tccgtaatct gatgtacgga atgcgtgact gcctcacgtt tggcgagccg atgaaaatag
    13261 gttatttacc agattccttt ggcatgtccg ggcaactgcc gcatatttac aatggatttg
    13321 gcattacccg caccatgttc tggcgcggat gttctgagcg ccacggtact gataaaaccg
    13381 agtttttgtg gcaaagcagt gacggtagcg aagtgacggc gcaggtgctg ccgctgggct
    13441 acgccatcgg taagtactta cctgccgacg aagacggatt acgcaaacgc ctcgacagtt
    13501 attttgatgt gctggaaaaa gcgtcagtaa ccaaagagat tttgctgccg aatggtcatg
    13561 accagatgcc attgcagcaa aatatcttcg aagtgatgga taagctacgt gagatctacc
    13621 ctcaacgtaa gtttgtgatg agccgctttg aagaggtatt tgagaagatc gaagcgcagc
    13681 gagataatct ggcaaccctg aaaggggaat ttattgatgg caaatatatg cgcgtgcatc
    13741 gcaccatcgg ttctacgcgt atggatatca aaattgccca cgcgcgtatt gaaaataaga
    13801 ttgttaatct gctggaaccg ctggcaacgc tggcctggac gttgggtttt gaataccacc
    13861 acggcttgct ggagaaattg tggaaagaaa ttttaaaaaa ccacgcccat gacagcatcg
    13921 gctgctgctg tagtgacaaa gttcatcgcg agattgtcgc ccgtttcgaa ctggcagaag
    13981 acatggcgga taatctgctg cgtttctata tgcgtaaaat tgccgataac atgccacaga
    14041 gtgatgccga caaacttgtg ctgtttaacc tgatgccctg gccgcgggaa gaagtgatca
    14101 acaccactgt gcggctgcgc gccagccagt ttaatttgcg ggacgatcgc ggtcagcctg
    14161 taccgtattt tattcgccat gcccgtgaga tcgatcctgg cctaatcgat cggcaaatcg
    14221 ttcattacgg taattatgat ccctttatgg agtttgatat tcagatcaac cagattgtcc
    14281 cttctatggg ctatcgcacg ctttatatcg aagcgaatca gcctggcaac gtgattgcgg
    14341 caaaaagtga cgctgaaggg atactggaaa atgctttctg gcaaattgcg ctcaatgagg
    14401 atggaacgct acaactggta gataaagaca gcggtgtgcg ctatgaccgg gtgttgcaaa
    14461 ttgaagaaag ctctgatgat ggtgatgaat atgactattc acccgcaaaa gaagagtggg
    14521 taattacttc ggcgaacgcg aaaccgcaat gcgagattac tcatgaggcc tggcagagta
    14581 gggctgttat ccgctatgac atggcggtgc cgcgtaatct gcatgaacgc agcgcaagac
    14641 aatccactgg cagagtaggg gtggagttgg ctgtcactct gagtgataac agcaggcgca
    14701 ttgatgtgga tatcaacctt gataaccagg ctgacgatca tcgccttcgc gtcctggtcc
    14761 ctacaccttt taacaccgac agtgttctgg ccgataccca gtttggttcg ctaacgcgcc
    14821 ccgtgaacga cagtgcaatg aacaactggc agcaagaagg ctggaaagaa gcgccggttc
    14881 cggtatggaa tatgctcaac tatgctgcct tacaggaagg gcgtaacggt ctggctgtct
    14941 ttagcgaagg gctacgtgaa tatgaagtca tcggtgaaga gaagaaaacc tttgccatta
    15001 cgttgctgcg tggcgtgggc ttactgggga aagaagatct gcttttaagg cctggacgtc
    15061 cttcaggaat aaaaatgccg gtgcccgatt cacaattacg tggctccttt agctgccgtc
    15121 tgagcttatt tagttataca ggtacgcctt cgaccgccgg tgtagcccag caggcgcgcg
    15181 catggctgac gccagtacag tgttacaaca aaatcccgtg ggatgcgatg aagttaaata
    15241 aagccggatt caatgtgccg gaaagctata gcttgctgaa aatgccccct gtgggatgcc
    15301 tgattagcgc tcttaaaaaa gcggaagacc ggcaagaagt ggtattgcga ctgtttaatc
    15361 cggcagaatc gacaacctgt gaggcaactg tcgcgttcag tcgtgaggtg atttcatgta
    15421 cgcaaacgat gatggatgaa agcatcagca cagcggaaag agataattta aaggaactgg
    15481 gggcattttt accggggcag tcacggacat ttagctaccg ccttggctga ttattgcgct
    15541 aaaagtgtat ctgagggggg caataatttc aggccatctt cggatggcct gagattgttg
    15601 ataaacaagt ttgttagctt acgcagatac cagatttgac ctgcgtgaga accgcaggcc
    15661 atttatcatt tcagttaccc cttaaacgtt aaccacggtc gaaatctgac cgctggctga
    15721 attaatccgg cttcttgttg ggcttcgata accggaccaa tatctttata agccgctggc
    15781 gcttcttcaa aacgtcgttc ttcgcgcaat gtgacgcatt gccacggcag cgttgactct
    15841 tcagtcgcgc gtgagcgcat ggcctgccga cgctggctgc gtcctgctcc gtgactgcat
    15901 gaccacagcc actccgggtt gccgcaacct accgcaagcc acgaataatc gcccattgaa
    15961 ccggggatta acgccagatt gcccactcgt gccggtgttg cgcctttgcg atgaatattc
    16021 atttcatgct cgcgaaggat gatgttatgt ggcacatcca ccaccagatg cgaattgtcc
    16081 tgatgaaaga gattggcaag acatacgcgc accatttcgg caattgtgac gcgatttaac
    16141 catgcgtaac gcgccgccat tcctatcgct tgtagatatt cctccgccag tccgcctgcc
    16201 agaccatata acccgctttg tggatgttta tgtccggtgg gccattcagc acgggcgcga
    16261 tccatccagt gtcttccgac gtaaaagcca acatctcgcg atccactgtg gatcatcacc
    16321 acaatatcgc ctgttttcag cccttgttgc caggctacat ggcgatcaac cacgctatca
    16381 acgacctgaa gttcaataaa atgattacct gaacccggcg tacccagaca aggatcgcgg
    16441 agtaatgcgc ggtccgttgc ctgcagggcg ggtggggcat aacgcgaatg actgttatgc
    16501 tcgttaagcc caatgcagcg gtcaatctct tgtaccattc gggggatatt cgtgcgtatc
    16561 cagagacctt ctgatggcaa ggtcgacacg caatctgccg gtccgccatc aaaaagcgcg
    16621 gtgaacgcct gtgcggataa cggaacatcg cgctcatttt gcagtagcac ttgcgttaac
    16681 cgctgagcga tgacgtcttt atgcgcttcg gcttgctcaa gtgttaatcc ggtcgtcagc
    16741 agccgcatgc cacaagaaat atcggtgccg atggcggcgg gaatgactaa atccggcgtt
    16801 gaggccacca ccgcgccaac ggggacgcca ttgccgggat gaaaatctgg cgtcgcgcac
    16861 gtctggcgca cataacctgg gtggtctggt agcttcactt ctgcaaaatc aagcagttgt
    16921 gttactgctt tttcttccag cggcagttcg tccggcagca gtacgcgggc actggcacct
    16981 gatggacgag ataatgaaac aatattgttg gcgcgggaaa gttggatccc tttgcgcaga
    17041 aaggctttat caagccttga aagatgatgc ataacgctgt cctgaaatat agacgttaaa
    17101 caaccccctg tatggcgagg gtaattttgt ctttccggtc agcagccagc aaaagggaaa
    17161 gaaaacgcgc gtagtgtatg tggcgatagc aagtgttaca agtggtaaat ctggcttatt
    17221 gttaaagagg aattatcttt tgtgaagtct tttaaggcca tgactcat
//
LOCUS       DAZGAP010000116        17173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGAP010000116 DAZGAP010000000
VERSION     DAZGAP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.117"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(129..833)
                     /locus_tag="ACHLAM_003317"
     CDS             complement(129..833)
                     /locus_tag="ACHLAM_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO7854793.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(970..1422)
                     /gene="moaE"
                     /locus_tag="ACHLAM_003318"
     CDS             complement(970..1422)
                     /gene="moaE"
                     /locus_tag="ACHLAM_003318"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO7854794.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1424..1669)
                     /gene="moaD"
                     /locus_tag="ACHLAM_003319"
     CDS             complement(1424..1669)
                     /gene="moaD"
                     /locus_tag="ACHLAM_003319"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO7854795.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(1662..2147)
                     /gene="moaC"
                     /locus_tag="ACHLAM_003320"
     CDS             complement(1662..2147)
                     /gene="moaC"
                     /locus_tag="ACHLAM_003320"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO7854796.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2150..2662)
                     /gene="moaB"
                     /locus_tag="ACHLAM_003321"
     CDS             complement(2150..2662)
                     /gene="moaB"
                     /locus_tag="ACHLAM_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO7854797.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2684..3673)
                     /gene="moaA"
                     /locus_tag="ACHLAM_003322"
     CDS             complement(2684..3673)
                     /gene="moaA"
                     /locus_tag="ACHLAM_003322"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO7854798.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3661..3805)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4070..4978
                     /gene="yvcK"
                     /locus_tag="ACHLAM_003323"
     CDS             4070..4978
                     /gene="yvcK"
                     /locus_tag="ACHLAM_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO7854799.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5170..7191)
                     /gene="uvrB"
                     /locus_tag="ACHLAM_003324"
     CDS             complement(5170..7191)
                     /gene="uvrB"
                     /locus_tag="ACHLAM_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO7854800.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7770..8447)
                     /gene="bioD"
                     /locus_tag="ACHLAM_003325"
     CDS             complement(7770..8447)
                     /gene="bioD"
                     /locus_tag="ACHLAM_003325"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7854801.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8440..9195)
                     /gene="bioC"
                     /locus_tag="ACHLAM_003326"
     CDS             complement(8440..9195)
                     /gene="bioC"
                     /locus_tag="ACHLAM_003326"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO7854802.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9182..10336)
                     /gene="bioF"
                     /locus_tag="ACHLAM_003327"
     CDS             complement(9182..10336)
                     /gene="bioF"
                     /locus_tag="ACHLAM_003327"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO7854803.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10333..11373)
                     /gene="bioB"
                     /locus_tag="ACHLAM_003328"
     CDS             complement(10333..11373)
                     /gene="bioB"
                     /locus_tag="ACHLAM_003328"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO7854804.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLASARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11460..12749
                     /gene="bioA"
                     /locus_tag="ACHLAM_003329"
     CDS             11460..12749
                     /gene="bioA"
                     /locus_tag="ACHLAM_003329"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO7854805.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTVAVNRAVQDETFFCQ"
     gene            12808..13284
                     /locus_tag="ACHLAM_003330"
     CDS             12808..13284
                     /locus_tag="ACHLAM_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO7854806.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNIHFHSLASASITAMFS"
     gene            13436..14719
                     /locus_tag="ACHLAM_003331"
     CDS             13436..14719
                     /locus_tag="ACHLAM_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO7854807.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14860..17121)
                     /locus_tag="ACHLAM_003332"
     CDS             complement(14860..17121)
                     /locus_tag="ACHLAM_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO7854808.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
BASE COUNT         3982 a         4663 c         4390 g         4138 t
ORIGIN      
        1 atttatggat acggaaaaca cttccgctcg gccataaggc atattttata cttggtactt
       61 gttaagtgtc gttttttcgt aagaaaaaaa tcccgacaca tggccgggat attgatggtg
      121 aaaaagaatt aacggcggtt gccgaagatc cgcaacaaca tcaggaacaa gttgatgaag
      181 tccagataca aggttaacgc gccaagaatg gaatatttgc gcaggttcga cgtgtcgcgg
      241 gtatcaatct gctcacccat atttttcaat ttctgcgtgt catacgccgt caatccgaca
      301 aagacaatca cgccaatgta ggtaactgcc cacatcaatg cttcgctttt cagccagaag
      361 ttgaccagcg atgccagcac aatgccgatt aacgccataa acagcatatt gccaaagccg
      421 cttagatcgc gcttcgtggt gtaaccgtac aggctcattg cgccaaacat tccggcagta
      481 acgacgaaag tactggcgat agaagcagcg gtataaacaa tgaaaatact ggaaagcgta
      541 agacccgtca gcgccgaata aagcataaag agcatcgtcg ttacacctgc gctcagcttt
      601 tgaatcatcg ctgataacac aataaccaat gctaattgcg cgatgatcag accgattaaa
      661 aagacacggt tagtgaacaa cagctccatc acagccgcgg aattagccgc ataccaggca
      721 acaaatgcgg tcagcaacaa gccaacggtc atccagccat agacttgcgc catataagtt
      781 tgcaagccag cccggggttg tacaattgaa tcagaacgtg ggaatctgtc catgacgatc
      841 tcctgaagat ataaggaata tcttaaggat actgcaaaat gatgaggctg tgcatcgacg
      901 cagcgtaaac gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc ttatccggct
      961 aacaaaaaac taccagcgtt ttgccgcctg ctgatcgctc tcccgagctt caacccagcg
     1021 gtcgccttcc ggcgtggctt cacgcttcca gaacggtgcg cgggttttga gataatccat
     1081 aataaactgc ccggcttcaa acgcactgct gcgatgcgca ctggtgacac cgacaaaaac
     1141 gatttcatct cccggccata attccccgat gcggtgaatc accgtgacgc gccccagcgg
     1201 ccagcggtta cgcgcttcat cgacaatttc tgccagtgct ttttcagtca tccccggata
     1261 gtgttcgagg gttaatgctt tgacgctgtc gccaaggtta tggttgcgca ccttaccggt
     1321 aaaggtgact accgcaccgt cttcatcacg ctccgccagc cacggatact cttctcctac
     1381 gctgaatggc tgcgggccga caacaatttt ggtttctgcc atcttaacct ccggttaccg
     1441 gcgggaagaa agccacttcg tcgccgtcag tcagcggatg gtcaagactc accagcgtct
     1501 ggttgacggc agccagcaat ttgccatctt ccagcgccag cgcccagcga tcgctctgcg
     1561 cagccagatg ttggcgtaac gcttcaacgg ttgggaaatc cgcagccact tcggttgcat
     1621 ctgttcccac caactcgcgc acctgggcga aaaaaagaac tttaatcatc cgcttccacc
     1681 ttaaagtcac ccgacttgcc gccgctcttc gccagcaaac gtaccggacc aattaccata
     1741 tctttttgca ccgctttgca catgtcataa atggtcagcg ccgccacgga ggccgcggtt
     1801 aacgcttcca tttcgacacc ggttttcccg gtcaggcggc ataaggtttc tatacgtacc
     1861 cgattgtgct ccggctcggc ctgtaaattg acttcaactt tgctgagcat cagcgggtga
     1921 cacagcggga tcagatccca ggtgcgtttt gccgcctgaa taccggcgat acgcgcagtg
     1981 gcaaatacgt cgcctttgtg gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc
     2041 atggtgacaa aggcttccgc ccgcgcttca cgcacggttt ccgctttggc ggagacatcc
     2101 accatgtgtg cttcgccagc ggcgttgata tgggtcagtt gcgacatact tatttcttca
     2161 aatgtggatg gaaattgcat ggacgtgtac gggcatcgag ctgcggcgcg atgatatttt
     2221 cccacgcggt gcggcacgct ttggtcgaac ccggcatggc gaaaatcagc gttttgttag
     2281 cgacgcccgc taccgcacga gattgcaacg ttgaggtgcc aatctcttca aacgacaaca
     2341 tccggaacac ttcaccaaaa ccttcaactt cacggtcgaa caacggcagc agcgcttcgg
     2401 gagcctgatc accttccgtc aggccagtac cacccgtaat caataccact tgtacatcgt
     2461 cgctggcgat ccacgcagat acctgagcgc gaatagcgta gcggttttct ttcacaatgg
     2521 ctttatcgac aacgtgatgg cccgcttcct gcgccgaatc gcgcagatag tgaccggagg
     2581 tatcgtcttc ttcaccacga cgattagaaa ccgtaagaat agcaatacgg gtcgggataa
     2641 attcagtgct tacctgactc atctgatctc tccttttgac gttttagccg ccaatgtacg
     2701 ataagttttg cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc
     2761 gcagcgccgc tgaaatacgc gcttccagcg cctgttgctg ggtatcgtct tccagcagat
     2821 cgcgcaggtt aacgccgcct tcaccaaaca ggcagagatg gagtttacca atggaggaaa
     2881 cgcgcaggcg gttgcaagtg gcgcagaagt ctttttcata cggcatgata aggccaatct
     2941 ctccggcgta atccggatga caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt
     3001 ggtggatcca gccgcgacgc agtagctcgt cacgcagaac ctgaccagag atgtgatgct
     3061 tacggaagag ctcgctgccc tcacccgttt ccatcaattc gatgaaacgc agctggatag
     3121 ggcgatgctg gatccagttc agaaaggtgt cgagctgatg atgattaaca tcacgcatca
     3181 gcacggtatt gactttgacc ttctcaaaac cggcctcaaa tgcagcatcg atccctgcca
     3241 tgacctggtt gaatttatcc tgcccggtaa tggcatgaaa ctggcgagcg tcaaggctat
     3301 caacgctgac gttgatgcca gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca
     3361 gacggtaacc attggtggtg accgcaatct ggcggatagc gtcgttttcc cgcacagcgg
     3421 cgatgatatc ggtaaagtcg cggcgtaaag acggctcacc gcctgtcagg cgcacttttt
     3481 cggtgcccag actggcaaag gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc
     3541 ctttattggt gacgccgctc ggtttgtagc catccggcag gcagtaggtg caacgaaagt
     3601 tacacacatc agtaatcgac aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca
     3661 gttgtgaagc catgtacacc tttccagata cgggaggcga agtcatttct tccttcgccc
     3721 tggtggcaat ctttacgagc attgtcacgg ccaaagcacc gtatcagttg acccaggtgc
     3781 agaggctaga gtgtttagtg gttaagccga tactagcgtg taaatgagat ttttaccatc
     3841 cacactttcg ctatgtaatc atgtatatag cgtcatgatc gtgctatttc gctacagaat
     3901 aagggaaaat ggcggtttta ctttgatata catcatcagg aaggcatagc gtacatcgtc
     3961 ctttagaggt agaagaaaat agtttgtatc ctcaatattg gcaggttaat tgctgtttcc
     4021 ccgcaatttg cgctactgta gcgcggataa tctgactcca ggaaactata tgcgcaatcg
     4081 tacgctggct gaccttgatc gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt
     4141 tctctcatca ctttcgtctt tgggttctcg tttaacgggt atcgtcacca ccacggataa
     4201 tggcggatcg acagggcgta ttcgccgctc agaaggcggt attgcctggg gcgatatgcg
     4261 taactgcctc aaccagctga taactgaacc cagcgtcgct tccgcaatgt ttgaatatcg
     4321 tttcggcggt aatggcgaac tttccgggca caaccttgga aatctgatgc tgaaggcact
     4381 ggatcacctt agcgtgcgac ctcttgaagc aatcaatctg attcgcaatc tgctgaaagt
     4441 ggacgcacat ttgattccga tgtcggagca tccggtggat ctgatggcga ttgacgatca
     4501 ggggcatgaa gtttacggcg aggtcaatat cgaccagtta actacgccga ttcaggagtt
     4561 actgttaacg ccgaatgtac ccgcaacgcg tgaggctgtt cacgctatca gtgaagcgga
     4621 tctcatcatt attgggcctg gcagttttta caccagcctg atgccaattc tgctgctgaa
     4681 ggaaatcgca caggcattac gccgcacgcc agcgccgatg gtctatatag gcaatctggg
     4741 gcgtgagttg agtttacctg cggctaattt gaagctggaa agcaagctgg caattatgga
     4801 gcagtatgtc ggtaaaaagg tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc
     4861 ggcggtgaaa gagcggattg tgattcagga ggtactggag gccagcgata ttccgtatcg
     4921 tcatgaccgc cagttgttac atagcgcgct ggaaaaggca ttacaggctt taggttaacg
     4981 gaaagccgaa ggaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg
     5041 atgcggcgtg aaagccttat ccggcctaca aaccgcgcta attcaatgta ttgcagagat
     5101 catgtaggcc tgataagcgt agcacatcag gcaattttgc ttttgtcatc tgtcttcttc
     5161 gttatcctgt cacgaagccg caatgaacaa ctcacgcagc tgatgcaact ggtcacgaat
     5221 ttgtgccgct tcttcgaact ccagattctg cgcgtgttgc atcatcaacc cttccagttc
     5281 atggattttc tgctgcaacg ctttaggcga catatccatc ggcacattat ccggctcaac
     5341 aatcggtcgc gattttcctc tgcccttcgc tttggttttg gcaatgttct gccccagcgc
     5401 caggatatcg accactttct tgttcaagcc ttgcggcgta atgccgtgtt cctcgttgta
     5461 cttctgctgt ttctcgcggc gacgttcggt ttcgccaatc gctttcgcca ttgatggggt
     5521 gatcttatcg ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac gaccaatggt
     5581 ctggatcaac gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga ggatcgccac
     5641 cagcgaaact tccggcatat ccagaccttc gcgcagtaag ttgatcccta ccaatacgtc
     5701 gaactcaccc agacgcaagt cgcggataat ctccatacgt tcgacggtgt cgatatctga
     5761 gtgaagataa cgcacgcgct caccgtgttc ttcgagatat tcagtgagat cttccgccat
     5821 ccgcttggtc agagttgtaa ccagtacgcg ttcgttaatt gccgctcgct gacgaatctc
     5881 cgaaagaaga tcatccacct gtgttgccac tggccgcact tcgataatcg ggtcgagtaa
     5941 gcctgttgga cgcaccacct gatccaccac atcgccaccg gatttttcca gctcgtaatt
     6001 acccggcgtc gccgaaacat agatggtttg cggcgctaat gcttcgaact cttcaaattt
     6061 cagcggacgg ttatccagcg ctgatggcag gcggaagccg tactccacca gtgtctcttt
     6121 acgcgcccgg tcaccgcgat acatgccgcc aatttgtgga atggtgacgt gagattcatc
     6181 gaccaccagc agcccatcag caggcaggta atcaaacagc gtcggcggtg gctcacccgg
     6241 tccacgaccg gagaggaagc gcgagtagtt ctcaatcccc gaacagtagc ctagctcgtt
     6301 catcatctcc agatcaaact gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt
     6361 gttttccaat agcactttac gtctggcggc cagctcttct ttgatctcct ccatcgcctg
     6421 gacgatgcgc tcgcgtggcg tgacgtagtg cgttttcggg tagatggtaa aacgtggaat
     6481 agtggaaaca atctgcccgg tcagcgggtc aaataacgac aatcgttcca cttcctcgtc
     6541 aaacagctcc acgcgaagtg caatgtcatc cgattctgcc gggaagatat caatcacctc
     6601 gccacgaaca cggaaagtac cacgctggaa ggcttgatca ttacgagcat attgcagctc
     6661 cgccagtcgg cgcaggatcg cgcgctgatc gataatcata ccgaccgtga gatggagcat
     6721 catcttgaga tataaatcag gatcgcccag accataaatc gcggaaacag acgccaccac
     6781 aaccacatca cgccgctcca gcatcgcttt ggtggcggac aaacgcatct gttcgatatg
     6841 ttcgtttacc gaggcatctt tctcaatgaa ggtgtcggaa ctcggtacat aggcctccgg
     6901 ctgatagtag tcgtagtagg agacgaaata ttccaccgcg ttttccggga agaattcttt
     6961 catttcgcca tacagctggg ccgccagcgt tttgttgggc gcaagtacca tggttgggcg
     7021 ctgaaggtca gcaatgacat tggcaatggt gaaggttttc ccggagccag tcacgccaag
     7081 taacgtctgg tgcgccaggc catcttccag cccctcttcg agacgtcgaa tcgcctccgg
     7141 ctgatcgcca gaaggtttaa aagcggaatt cagtttgaac ggtttactca tgagtcgcta
     7201 cctgaaggag ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat tatgccaaca
     7261 aattatactg gataaaaaaa cagttcatca ccataatatt tctgttacag cgtaaactcc
     7321 gctgtcaatc atgagcaaaa tttactctgc ggcgagataa aactccggcc ttaccgggtt
     7381 atccccaaag caacggcttt tttaacattt gtcaagatga ttgatgacag ttttatggca
     7441 agagatgcct gttcagtgac tgccattgca attatctaac ctgttaaaaa ataaaagata
     7501 tttctttgag ccgtctttaa cgccaattcg cgtgcaagcc gcgtattctc tcgcttgcct
     7561 cgtgttttct aactctaata cacatggtta tccacaggaa tagtggataa ctgtctgcag
     7621 cccctatccc ccgccgcctg ggcaattccc acattcaccg catgatgcgg aggtaaaatt
     7681 tttttcgtga tttttttaga atttaattgg ttaaattgca gtcaatcgga gacacgatct
     7741 cgctcgcaat ttaaccaatt acaggatgac tacattaagg caagattgat gtactttccg
     7801 gttgccgcat tttctggatt ttctgcaagc cagggaattt cgcccagcag cggcgcggga
     7861 atcatgcggg tgagcgtggt catatattca gcgtgacgtt ttcccggagg cgtgacatcg
     7921 ttcgccaccc accccgccag agtcagtccg gcgtgtagta ttgcctgcgc agtcaacatc
     7981 gcgtgattaa tacagccgag tttcacacca actaccagta tcaccggcag ttgttcctgt
     8041 gttacccaat ccgcaaaagt gaaagtgtca gaaagcggcg taaaccagcc gccagcacct
     8101 tccactaaca cccagtcagc ctgttgttca agcgcgcgta atccggcgct cattaccaat
     8161 gattctatcg gcctgccctc ttgcgcgctg atgatatgcg gcgaggtggg ttctgcgaag
     8221 gtgtaaggat ttaccgttgc gtaatccagc tgcaggctac tgttgcgctg taacgccagc
     8281 gcgtcactat tacgtaagcc tttcggggtc ttttcgctgc cagaggccac cggtttataa
     8341 cctgccgtcc ggtagcctgc tgcctttgcg gcttgtaaaa gcgcgcagct ggcgacagtt
     8401 ttccccactt cggtatccgt tccggtgaca aaataacatt tactcacgag caatcactcc
     8461 caaaaaaaga tgatacgtca gaggatatcg cccctgttgt tgcggccagg ctagttgcaa
     8521 tcgctgcaac tgcgaacgcg ttaatattcg cgggtcgcgc ccttcatgaa gatgcgtggc
     8581 accgatgcct ttcagcgaac gcatggcact gagcgcatca tcaaaccaca gcgtaatggg
     8641 ctgaatataa tgttgataat gcacggcgtt tagcgactgt tcgattttat ctggcggtaa
     8701 aaagcgatta gcatgcgggc gctcgtccac cgcctgccac gcctgatgca gttcgggtaa
     8761 cgatccctgc accagcgtgg taaacgcgac cacgcctccg gggcgcacca cccgatacag
     8821 ctcgcggagt gccatggata aattaccgca ccactgcacc gcgagattgc tccatgcaag
     8881 atcgaacgtt gcagtcgcta acggcaggga ttcgatatct cccgccagat aatggtctgc
     8941 ggcatccttc tggcgtgcct gaacaagcat tggcggagag agatctaacg cagtcacctg
     9001 cgcgtgacgc tctcgccagc ggcggctcat ccagcctggt ccacaacccg catccagtac
     9061 gtgggtgtat ttacgctgtg ggagcattgc cagtaaggcg tcagcactct ggcgctgtag
     9121 atcggcgtgt tgctcatagt gtgcggctgc ccgtccaaat gccgctgcaa tggcttgttt
     9181 attaaccgtt gccatgcagc acctccagca gacggtcgat atcctgcatt tcatgcgcgg
     9241 ctgttagtgt taagcgcagt cgcgcagtac cagcaggtac ggttggcggg cgaatcgccg
     9301 tgacccagca gccttgctgg cgcagttttt ctgccagttg taacgcacgg ctgttatcac
     9361 caacaatcaa tggctggatg gcgctgcatg aatcagcaag cgtaaacggc aaatcctgta
     9421 ctccggcacg aaaacgcgta atgagtgccg ccagtttttc gcgccgtgta tcaccctcat
     9481 cactgcgaat gaccgccagc gatgcacgta atgcctgcgc ctgagcgggc ggcatactgg
     9541 tgctgtaaat aagatggcgg gcaaattgca ccagataatc cgccaccgta tcggagcaaa
     9601 gcaccgctgc cccgctgacg ccaaatcctt tgccaaaagt caccaccagc aattctggtt
     9661 ttaccttttg cagccagcag ctgccgcgcc cctgctcccc gataacgccc gtgccgtggg
     9721 cgtcatcgac catcaaccag ccatcgtgct gttgcgttac ctgctggatt tcctccagtg
     9781 gcgcactatc gccgtccatg ctgaacacac cttctgtcac taccagttgc tgccccggac
     9841 agggggaagc aagcagtcgc gccagatgag tgacatcgtt atgagcaaaa cggcgaagcg
     9901 gcgacgggct taaacttgca gcctccagca atgaggcatg gctaagccgg tcggcaacaa
     9961 tacggtcctc tttcgccatc atcgcggcaa tgactgcctg attagccgca aaaccggaga
    10021 taaacagcag cgcccgcgaa tagcccagcc actcggccag ttcttcttcc agcgcctgat
    10081 gtgccacgct ataaccgctg acgtgaccgg agccgccgct accgacgcca aattgctccg
    10141 cactctgctt ccaggcacgg ataatttgcg gatgatggct taaacctaaa taatcgttac
    10201 tggaaaagtt cagatactgg cgatcgtccg ccaccagcca gcgtccggcc ccttgcgcca
    10261 ccggataacg gcgacgcagg gcatcggcag cacgccgcgc atcgagcgcc gcgttgattt
    10321 tctcctgcca gctcatagtg ctgccgcgtt gtaatattcg tcagtgtccg gggtcatcag
    10381 tgcctgctcc agacgctgct gttgttcgtt atcccctgcc agcacggcag tttgctgtgg
    10441 atttagcccc agtttgcgga acagttgcag gtctttatct tcttccggat tcggcgtggt
    10501 tagcagtttg caaccgtaga aaatcgagtt agccccggcc ataaagcaca tcgcctgagt
    10561 ctgttcattc atctgctcgc gtccggcaga aaggcgcacg tatgaggtcg gcatcatgat
    10621 ccgcgctacg gcaatggtgc gaataaaatc aaaggcatcg acatcatcgt tatctgccag
    10681 tggagtacct ttcaccttca cgagcatgtt gattggcacg ctttccggcg gcgtcggcag
    10741 gtttgccagt tgcagtaata atccggcacg atcttttacc gtttcgccta aacccacaat
    10801 gccacctgag cagactttga tcccggcatc gcgcactttt tccagcgtat caaggcgttc
    10861 ctgataagta cgtgtggtga tgatattgcc gtaaaactcc ggcgaggtgt cgaggttatg
    10921 gttgtagtaa tccagcccgg cattcgcaag gcgctgcgcc tgagattcac tcaacgtacc
    10981 cagcgtcata cacgcctcca gccccatcgc ttttactccc tgcaccattt gttccaggta
    11041 gggcatatcg cgttcgtggg gattcttcca cgccgcgccc atgcagaagc gcgtcgatcc
    11101 tgccgctttc gctttgcgcg ccgacgccag cacctgttca acttccatca accgctcggc
    11161 ttccagcccg gttttgtagc gcgagctttg cgggcagtat ttgcaatctt ccggacaagc
    11221 tccggtctta attgacagca aagtgctgac ctgcacctga cgtggatcga aatgctgacg
    11281 atgcacctgc tgcgcttcaa acagcagatc cagcaacggt ttttcaaata attctgtgac
    11341 ttgcgacaat gtccagcgtg ggcggtgagc catggggctt ctccaaaacg tgttttttgt
    11401 tgttaattcg gtgtagactc gtaaacctaa atcttttcaa tttggtttac aagtcgatta
    11461 tgacaacgga cgatcttgcc tttgaccaac gccatatctg gcacccatac acatccatga
    11521 cctccccgct accggtttat ccggtggcga gcgccgaagg ttgcgagcta attttgtctg
    11581 acggcagacg cctggttgac ggtatgtcgt cctggtgggc ggcgatccac ggctacaatc
    11641 acccgcagct taatgcggcg atgaagacgc aaattgatgc catgtcgcat gtgatgtttg
    11701 gtggtatcac tcatgcgcca gccattgagc tgtgccgcaa actggtggcg atgacgccgc
    11761 aaccgctgga atgcgttttt ctcgcggact ccggttccgt agcggtggaa gtggcgatga
    11821 aaatggcgtt gcagtactgg caggccaaag gcgaagcgcg ccagcgtttt ctgaccttcc
    11881 gcaatggtta tcatggcgat acctttggcg cgatgtctgt gtgcgatccg gacaactcaa
    11941 tgcacagcct gtggaaaggc tatctgccag aaaacttgtt tgctcccgcc ccgcaaagcc
    12001 gcatggatgg tgaatgggat gaacgcgata tggtgggctt tgcccgcctg atggcggcac
    12061 atcgtcatga aatcgccgcc gtgatcattg aaccgattgt ccagggcgca ggcgggatgc
    12121 gcatctacca tccggaatgg ttaaaacgaa tccgcaaaat gtgcgatcgc gaaggtatct
    12181 tgctgattgc tgacgagatc gccaccggat ttggtcgtac cggcaaactg tttgcctgtg
    12241 aacatgcaga tatcgcgccg gatattttgt gtctcggtaa agccttaacc ggcggcacaa
    12301 tgaccctttc cgccacgctc accacgcgcg aggttgcgga aaccatcagt aacggcgaag
    12361 ccggctgctt tatgcatggg ccaactttta tgggcaatcc gctggcctgc gcagcagcaa
    12421 acgccagcct ggcgattctc gaatctggcg actggcagca tcaggtggcg gctattgaag
    12481 ggcagctacg cgagcaactt gcccccgcct gtgacgccga aatggttgcc gatgtgcgcg
    12541 tactgggggc aatcggcgtg gtcgaaacca ctcgtccggt gaatatggca gcgctgcaaa
    12601 aattctttgt cgaacagggt gtctggatcc ggccttttgg caaactgatt tacctgatgc
    12661 cgccctatat tattctcccg caacagttgc agcgtctgac cgtagcggtt aaccgtgcgg
    12721 tgcaggatga aacatttttt tgccaataac cgggagtcag cgtgaggggt tctggctaca
    12781 ctttctgcaa actagaaagg agggttcatg aaactcatca gtaatgatct gcgcgatggc
    12841 gataagttgc cacatcgtca tgtctttaac ggcatgggtt acgatggcga taatatttca
    12901 ccgcatctgg cgtgggatga tgttcctgcg ggaacgaaaa gttttgttgt cacctgctac
    12961 gacccggatg cgccaaccgg ctccggctgg tggcactggg tagttgttaa cttacccgct
    13021 gatacccgcg tattaccgca agggtttggc tctggtctgg tagcaatgcc agacggcgtt
    13081 ttgcagacgc gtaccgactt tggtaaaacc gggtacgatg gtgcagcgcc gccgaaaggc
    13141 gaaacccatc gctacatttt taccgttcat gcgctggatg tagaacgtat tgatgtcgat
    13201 gaaggtgcca gcggcgcgat ggtcgggttt aacatccatt tccactctct ggcaagcgca
    13261 tcgattaccg cgatgttcag ttaattactc tgccagatgg cgcaaagcca tctggtatca
    13321 cttaaaggta ttaaaaacaa ctttttgtct ttttaccttc ccgtttcgct aaagttagta
    13381 taaaaaagca ggcctcaacg gattcatttt tctatttcat agcccggagc aacctgtgaa
    13441 cacattttca gtttcccgtc tggcgctggc attggctttt ggcgtgacgc tgaccgcctg
    13501 tagctcaaca ccgcccgatc aacgtccttc tgatcaaacc gcgcctggta cctcttctcg
    13561 cccgattctg tcggcaaaag aagcgcagaa tttcgatgct caacactatt ttgcatccct
    13621 gacaccaggt gcggcagcgt ggaatccttc cccgattacc ctgcctgcac aacctgactt
    13681 tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc acgattcagg cggcggtaga
    13741 tgcggcaatt atcaagcgta ccaacaagcg ccagtatatt gccgtgatgc ctggtgagta
    13801 tcagggaacg gtatatgtcc ctgccgctcc gggtggaatt actctgtacg gtacaggtga
    13861 aaaaccgatt gatgtgaaga ttgggctttc ccttgatggg ggcatgagcc ccgccgactg
    13921 gcgtcacgac gtcaacccgc gcggcaaata tatgccaggt aaaccagcgt ggtatatgta
    13981 cgatagctgc cagagcaaac gcagcgacag tatcggcgtt ctctgctctg cggtcttctg
    14041 gtcacaaaac aatggactgc aactgcaaaa cctgaccatc gaaaacacgc tgggcgatag
    14101 cgtagatgcg ggtaaccatc cggcggtggc actgcgtact gatggtgacc aggtacagat
    14161 taacaacgtt aacattctcg gtcgtcagaa caccttcttt gtcaccaaca gcggtgtgca
    14221 gaaccgtctg gaaacgaatc gtcagccgcg tacgctggtg accaacagct acattgaagg
    14281 ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc gataataccg aattccgcgt
    14341 ggtgaactca cgtactcagc aagaagcgta tgtgtttgca ccggctacgc tgtccaacat
    14401 ttactacggt ttcctcgccg taaacagtcg tttcaatgct tccggtgatg gcgtggcgca
    14461 actgggccgc tcgctggatg ttgatgccaa taccaacggt caggtggtga tccgtgatag
    14521 cgccatcaac gaaggtttta acacagccaa accgtgggct gatgcggtga tttccaatcg
    14581 tccattcgcg ggtaacaccg gcagcgttga tgataacgac gaagtacagc gcaatctgaa
    14641 tgacactaac tacaaccgca tgtgggaata caataaccgc ggcgtgggta gcaaagtggt
    14701 tgcagaggcg aagaagtagg agctgttaac tatgtgccgg atgcggcgta aacgccttat
    14761 ccggcctacg gttcgatgcg atttgtaggt cggataagat gcgccagcat cgcatccgac
    14821 aataagtgcc ggatgcgacc tacattcaca tggcgctttt tacatctgac ggtttttatt
    14881 gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca gactttccat
    14941 atagagcgta atctccgtta ccggtgcatc ttcatggatc acataacctt taaacgtcgt
    15001 tccaggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc ccacttcaaa
    15061 cgttggtgct tccgccattt gcagcggtaa catcccccag ttgatgacgt tagaacggta
    15121 gcgtttagtc gcgtattcct cggcaatatt cgccagaccg ccaatcacac gctggcagct
    15181 cgccgcctgc tcacgcgcag aaccatcgcc tggtttcacc gcatagacca tgctgccaat
    15241 ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc gcgcaaacac
    15301 ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag cagtcgcttt
    15361 acttctgcca acataaccgg gatcgcggcg tgacagggta aactccgcca gaccaatcgg
    15421 atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg tggtgacttc
    15481 gtcgaggatc ttcgagcaca ctttgaggac gatattgtca gtcagcgcgc ccaattccgg
    15541 ccagtctttg atgttcggtc cgtagatcag cggttgctgg gtcgcccctt tcacaaagcc
    15601 ttgatagacg cgatttttat acggcgttac atcgaaggcg tactccggca cgttgtccca
    15661 gcaatcgagt tcgctggcag aggttaaata gccaccgttt gccgcagttg cagcgataga
    15721 acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag agccttcgcg
    15781 gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg gcgtatcgcc
    15841 cgcaccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc caatcaaatc
    15901 tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg ggtaaaccgc
    15961 caatgagaag gtatcgttgc cgcaggattg accgcgcagc gcattcgccg ccgcgatgac
    16021 gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt tcaggcgacc
    16081 attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc gttcggactc
    16141 aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agcgtgtcga tttcatacac
    16201 gttgctcggg tggaatggca gcgcaatcat tggtttgatg gcgcttaaat caacgctgat
    16261 gcagccatcg tagtacgcca ttggttgagg gttaagttgg cagtaatcct ggccgcgacc
    16321 gtgcagcgcc agccagttat ggacttcttc atcggtttgc cagacagaac ttaaacaggt
    16381 cgtttcagtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg ctgcaacacc
    16441 gggacctacg aactccatca ctttgttttt gacgtaaccg tttttgaaca cggcaccgat
    16501 gatagccagc gccacatcct gcggccccac atacggcgca ggttttccgg tcagatgcac
    16561 cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt ttaccaactc
    16621 gccgccgccc tcgccaaccg ccattgtccc taacgcaccg taacgggtat ggctgtctga
    16681 cccgaggatc attttgccgc cgcccgccat catttcacgc atatattgat ggatgaccgc
    16741 aatatgcggt ggcacaaaaa taccgccata acgctgggcc gccgataaac caaaaacatg
    16801 gtcatcaccg ttaatggtgc cgcccacagc acagagtgag ttatggcagt tggtcagcac
    16861 atacggcagc gggaaacgtt ccataccgga cgctttagcg gtctgtacaa taccaacaaa
    16921 ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca tatttccgga
    16981 cgtattatga gaagagagaa tagaccaggc aatagtgcct ttttttgctt cttctttttt
    17041 tatttcgccg gtgaaatgtt cttcggcaat tatttcgtta ttgctggcga gaaacacgcc
    17101 tttttcagat aacttgatca tcaaatgctc cagatatctg cgcaccgtga agtgcgcaga
    17161 aagataataa atg
//
LOCUS       DAZGAP010000117        17119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGAP010000117 DAZGAP010000000
VERSION     DAZGAP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.118"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..1791
                     /locus_tag="ACHLAM_003333"
     CDS             106..1791
                     /locus_tag="ACHLAM_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO7854809.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(1967..2503)
                     /gene="rcdA"
                     /locus_tag="ACHLAM_003334"
     CDS             complement(1967..2503)
                     /gene="rcdA"
                     /locus_tag="ACHLAM_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO7854810.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            2587..3795
                     /locus_tag="ACHLAM_003335"
     CDS             2587..3795
                     /locus_tag="ACHLAM_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854811.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3795..4610
                     /gene="ybjI"
                     /locus_tag="ACHLAM_003336"
     CDS             3795..4610
                     /gene="ybjI"
                     /locus_tag="ACHLAM_003336"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO7854812.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFSTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            4695..4979
                     /gene="ybjH"
                     /locus_tag="ACHLAM_003337"
     CDS             4695..4979
                     /gene="ybjH"
                     /locus_tag="ACHLAM_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO7854813.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(5020..6252)
                     /gene="mdfA"
                     /locus_tag="ACHLAM_003338"
     CDS             complement(5020..6252)
                     /gene="mdfA"
                     /locus_tag="ACHLAM_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO7854814.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHVWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            6537..7133
                     /gene="ybjG"
                     /locus_tag="ACHLAM_003339"
     CDS             6537..7133
                     /gene="ybjG"
                     /locus_tag="ACHLAM_003339"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7854815.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWSMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7191..7949
                     /gene="deoR"
                     /locus_tag="ACHLAM_003340"
     CDS             7191..7949
                     /gene="deoR"
                     /locus_tag="ACHLAM_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO7854816.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(7996..9198)
                     /gene="dacC"
                     /locus_tag="ACHLAM_003341"
     CDS             complement(7996..9198)
                     /gene="dacC"
                     /locus_tag="ACHLAM_003341"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7854817.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            9445..10071
                     /gene="gstB"
                     /locus_tag="ACHLAM_003342"
     CDS             9445..10071
                     /gene="gstB"
                     /locus_tag="ACHLAM_003342"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO7854818.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(10068..11183)
                     /gene="yliI"
                     /locus_tag="ACHLAM_003343"
     CDS             complement(10068..11183)
                     /gene="yliI"
                     /locus_tag="ACHLAM_003343"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO7854819.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESDARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(11294..11677)
                     /gene="bssR"
                     /locus_tag="ACHLAM_003344"
     CDS             complement(11294..11677)
                     /gene="bssR"
                     /locus_tag="ACHLAM_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO7854820.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            11890..13215
                     /gene="rimO"
                     /locus_tag="ACHLAM_003345"
     CDS             11890..13215
                     /gene="rimO"
                     /locus_tag="ACHLAM_003345"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO7854821.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            13441..13827
                     /locus_tag="ACHLAM_003346"
     CDS             13441..13827
                     /locus_tag="ACHLAM_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7854822.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            13817..14146
                     /locus_tag="ACHLAM_003347"
     CDS             13817..14146
                     /locus_tag="ACHLAM_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7854823.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(14216..15544)
                     /locus_tag="ACHLAM_003348"
     CDS             complement(14216..15544)
                     /locus_tag="ACHLAM_003348"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7854824.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(15552..17119)
                     /locus_tag="ACHLAM_003349"
                     /pseudo
     CDS             complement(15552..17119)
                     /locus_tag="ACHLAM_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         4345 a         4248 c         4095 g         4431 t
ORIGIN      
        1 gtttaggtgc gctgaatgaa tctgcgccct gaattctggt aaaaaacatt atcgtaaatt
       61 accatttctt tcaacagctt actagtaaac aagaagttag cctccgtgaa tataaacgtc
      121 gccgaattgt taaatgggaa ttacattctg ttattatttg tggtcctcgc gcttgggcta
      181 tgtctcggga aattacgact tggttcgatc caactgggta attccattgg cgttttagtc
      241 gtatcgctgt tattaggcca acaacatttc agcattaaca ccgacgcgct taatcttggc
      301 tttatgctgt ttattttctg cgtcggggtc gaagccggac cgaacttttt ttccattttt
      361 tttcgcgatg ggaaaaatta cctaatgtta gcactggtga tggttggcag tgcgctggtc
      421 atcgccttag ggttaggtaa gctgtttggc tgggatattg gcctgacggc cggtatgtta
      481 gcaggctcta tgacgtcaac accggttctg gtcggtgctg gcgatacact gcgtcattcc
      541 ggcatggaaa gcaggcagct ctcattggca ctggataatc tgagcctcgg gtatgcctta
      601 acctatttaa tcggcctggt gagtttgatt gttggtgcgc gttacttgcc gaaattgcag
      661 catcaggact tacagaccag cgcccagcaa atcgcccgcg aacgtggcct ggacacagat
      721 gccaaccgta aggtttattt gccggtgatc cgcgcctacc gcgtcggccc ggagctggta
      781 gcctggaccg acggcaaaaa tctgcgtgaa ctgggtattt atcgacaaac tggctgttac
      841 attgaacgta ttcggcgtaa cgggattctg gcaaatccag acggtgatgc cgtactacaa
      901 atgggcgatg aaatagcgtt ggtaggctat cctgacgccc acgcccgact cgatcccagt
      961 ttccgtaatg gcaaagaagt tttcgatcgt gaccttctcg acatgcgtat cgtcactgaa
     1021 gaagtggtcg ttaaaaacca taacgctgtt ggcaaacgcc tggcacaact gaagttgacc
     1081 gatcacggtt gcttccttaa ccgcgtcata cgtagccaga ttgagatgcc gattgatgac
     1141 aacgtcgtgc ttaacaaagg tgacgtttta caagtcagcg gcgatgcccg ccgggtaaaa
     1201 accatcgccg atcgcatcgg ctttatctcg attcacagcc aggtcactga cttgctggca
     1261 ttctgcgcct tctttgttat tgggctgatg atcgggatga tcaccttcca gttcagcaca
     1321 ttcagtttcg gtatggggaa cgctgccggg ttgttattcg ccggaattat gctgggcttt
     1381 atgcgtgcta accacccgac cttcggttac attccgcagg gtgcattaag catggtgaag
     1441 gagttcggct tgatggtgtt tatggcaggc gttggtctga gcgctggtag cggtattaat
     1501 aacggcctgg gcgcgattgg cggtcagatg ttgattgccg gattgattgt cagtctggtg
     1561 ccagtggtta tctgcttctt gttcggtgct tatgtattgc gaatgaaccg cgcactgttg
     1621 ttcggcgcaa tgatgggcgc acgcacctgc gcgccggcaa tggagatcat cagtgataca
     1681 gctcgcagta atatcccggc actgggctat gcgggcactt acgcaatcgc caacgtcctg
     1741 ctgacgctgg cagggacaat catcgtcatg gtatggccag gattaggata aaactgaagt
     1801 tgccctgaaa atgaaatttt ttttgcacaa ccgcagaact tttccgcagg gcatcagtct
     1861 taattagtgc cactgctttt ctttgatgtc cccattttgt ggagcccatc aaccccgcca
     1921 tttcggttca aggttgatgg gttttttgtt gtctaaaatt tatctactac cctgcaaccc
     1981 tctcaaccat cctcaaaatc tcctcgcgcg atagcggctt acggtcggtg acaaaatgca
     2041 gcgtcatccc ctcaataaac gcatcaagcg cgcgggcggt tccgggttca aaccattgtt
     2101 cgagcgtttg ctgactgcgc tgcatccagt tttgcattac cgtttttaat agcggttttc
     2161 ggctagccag cgcgtagagc tggtacatca gctccatgtt atccggcgtt gcaacctgtg
     2221 agctgtaaat catatcggtg atagcctggc atgcgcccgg agcatcacta acatcgctaa
     2281 aaaatgcctg atattgccgg gacatgatct cagtaaaact gctgaacgcc tccagcaaca
     2341 actcatcaat tcctgaaaaa tagtaggtca tcgaccccaa cggtaccccg gcaagggtag
     2401 caattttgcg gtgcgtaaca gcatgtattc cataaagttt caccgcctcc agcgtggcct
     2461 ggataatttt ttctcgccgt tgcggatcgt tagcgcgacg catgtcattt cctctctctg
     2521 caattgtgta caaatgtaca caaccttgct aatgttgtct tccctcttct tattttgacg
     2581 tgatctatga ccgtaaattc ttcacgtaat gcattgaaac gccgaacctg ggcgctgttt
     2641 atgttcttct ttttgccagg cctgttaatg gcgtcctggg caacccgtac gcctgctatc
     2701 cgcgatattc tttctgtttc gatcgctgaa atgggcggag tcctctttgg tctgtcgatc
     2761 ggttcaatga gcggtattct ctgctcggcg tggttagtga aacgctttgg gacgcgtaat
     2821 gtcatcctgg tcacgatgtc ctgcgcattg atcgggatga tgatactaag tctggcactc
     2881 tggctgacat cgcccctact cttcgcggtt ggtctcggcg tctttggggc aagttttggt
     2941 tctgcggaag tggcgataaa cgttgaaggt gctgccgttg agcgagaaat gaataagacg
     3001 gttttgccga tgatgcacgg tttttatagc ctgggcacgc tggcaggcgc tggtgtcggg
     3061 atggcactaa cggcctttgg cgttccggca acggtgcaca ttttattggc ggcgctggta
     3121 ggcatcgcgc ctatttatat cgccattcag gcaatccctg atggtacggg caaaaatgct
     3181 gccgatggca cccagcatgg tgaaaaaggc gtaccttttt atcgcgatat ccagttgctg
     3241 ctgattggtg ttgtggtgct ggcgatggcc tttgccgaag gttctgccaa cgactggtta
     3301 cccttattaa tggttgacgg tcacggtttt agccctactt ccggctcgct gatttatgcc
     3361 ggttttaccc tggggatgac cgttggacgc tttaccggcg gttggttcat tgaccgttac
     3421 agtcgcgttg ccgtggttcg ggccagtgca ctaatggggg cgttgggtat tgggctgatt
     3481 atttttgtcg atagcgcctg ggttgctggg gtgtctgttg tactttgggg actgggtgcc
     3541 tcgttgggct tcccgctgac catttctgcc gccagcgata ccggccccga tgcaccaacc
     3601 cgcgtcagcg tggtagctac gaccggttat ctggctttcc tcgtcgggcc gccactgctg
     3661 ggctatctcg gcgaacatta tggattacgt agtgcaatgc tggttgtact ggcgctggtt
     3721 attctcgcgg ctattgtcgc gaaagccgtc gccaaacccg ataccaaaac gcagacggcg
     3781 atggagaata gttgatgagc attaaattaa ttgcggtaga catggatggt actttcttaa
     3841 gcgatcaaaa aacctataac cgtgagcggt ttatggctca gtatcagcaa atgaaagcac
     3901 aaggcattcg ctttgtggtc gccagcggga atcaatatta tcagttgatc tctttctttc
     3961 cggaaattgc taatgaaata gcctttgtgg ctgaaaacgg cggctgggta gtgagcgaag
     4021 gcaaagatgt ttttaatggc gagctgtcga aggatgcgtt ttctactgtc gtggaacatt
     4081 tgctgacgcg cccggaagtg gaaattattg cctgcggaaa aaatagcgcc tatacactca
     4141 aaaaatatga cgatgccatg aaaacggtgg cggaaatgta ttatcaccgt ctggaatacg
     4201 tcgataactt tgacaactta gaagatatct tctttaagtt tggtctgaat ctttccgatg
     4261 aactgattcc acaagtacaa aaagcattac atgaggccat cggcgatatt atggtgccgg
     4321 tccacaccgg caacggcagc atcgatctga ttatccccgg cgtacataaa gccaatggcc
     4381 ttcgccaact gcagaaatta tggggaatag acgacagcga agtggtggtc tttggcgatg
     4441 gcggtaacga tattgagatg ctgcgtcagg caggctttag ttttgcaatg gaaaatgccg
     4501 gcagcgcggt cgtcgcagcg gcaaaatacc gggcaggctc caataaccgt gaaggcgtac
     4561 tggatgtgat cgataaagtg cttaaacacg aagcgccatt taaccaataa aacggcagca
     4621 cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta acccgctgca
     4681 ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt ttaatgggct
     4741 ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga tacaaagtgg
     4801 ttgtggttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact gtgccgcaaa
     4861 ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat gttcgccatc
     4921 ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat caccattaat
     4981 tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt tacccttcgt gcgaatttcc
     5041 catctgttta tcttttaaaa agataaccat cagcaacagc cacaaaattc cgttgacaag
     5101 gttgaagaga ttaaacagtc cgttgccccc gttcagccag acatgtttgc tgatttcaat
     5161 accgacggta aagatcagca tttgcagcat ccccatcgcc gcagaaaccg tacctttact
     5221 catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac caataccgaa
     5281 agcataaata cttaacccgg cggtcatcca taaatacgcg tgcgatgaga taaccgttgc
     5341 cgcagcagcg accaacagac caatcataat cggccagccg cccataataa tcagcgaacg
     5401 tacggtgcgg cgcgaggtca gacgcgctaa cagcaagtta cccgcaatta acgccccgaa
     5461 aataggcact tgcagcaagc catattcata gctgctcaac tgctcaccgg taataatgat
     5521 aatcggcgac tgggcgatcc acgccaacaa cggcaggcta acgaatccca gcgccagcgc
     5581 ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga gttctttcag
     5641 cgacagtttc tcgcctatac gcgtggcggt ttcaggcatg gctcgttgca gaccgaaaaa
     5701 ggagatcgct gccaatgcgg caaacaagac aaacatccct tcccagggca gcacatggat
     5761 ccacgccgcg cccaccagcg gaccaagtag tggagcaatc agcgccacgt tcgccattag
     5821 cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc tgaattgcgg cgtatcccac
     5881 agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagggtga attgttcaat
     5941 gttttgcgcc agtaatattg ccagacaggt gacgataaac cacaccactc ccgccagcat
     6001 caccggacgg cggccaatac gatccgacaa cggccccagg agccattgta aaaacatccc
     6061 gcccgccaga tacgcagtca tcgaagtagg aacccaatca atgcccgcct gatattgttc
     6121 caccacggcc aacataccgg gttgaatcat atcgttgccg atataggttg aaaattcgta
     6181 aagcaccaga cagagaggga aaagtaacgc ctgacgtcca agcctggcac cggaagctaa
     6241 tttattttgc atgcaatttc ttcgccaata ataatcgcgc aaagtttaat aaaagcgcag
     6301 ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa gcacattctt acattattgc
     6361 gagtatagct acgctttctt taagttttat ttaacctttg cccgttacaa tcacccaccg
     6421 taaacaggcc gcttgaggga aataagacga tgccgcttta cccagtttaa cctgcacttt
     6481 attctcaacg acttgcctgt attggctccc ttttaatcac tttgcgtcgg gaagttatgc
     6541 tggaaaattt gaatctctct ctattctctc ttattaacgc gacgccagac tctgcgccgt
     6601 ggatgatctc gttggcgatt tttattgcaa aagatttgat taccgtggtg ccgttgctgg
     6661 ccgcggtact ttggctgtgg gggcttacag cacaacggca actggtgata aaaatcgcta
     6721 tcgcgctggc ggtcagcctg tttgtgtcct ggtcgatggg gcatcttttt ccgcacgacc
     6781 gaccctttgt cgaaaatatc ggctataact tcctgcatca tgcggcggat gactcattcc
     6841 cgagcgatca cggtacggtg attttcacct ttgcactggc atttttatgc tggcatcgcc
     6901 tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat tgcctggtcg cgcgtttatc
     6961 ttggcgtcca ctggccgctg gatatgctcg gtggattgct ggcaggtatg attggctgcc
     7021 ttagcgccca gattatctgg caagcgatgg ggcataaact ctatcaacgt ctgcaatcgt
     7081 ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg ggtgcgtgac tgatctctgc
     7141 taaaaagtgt agtattgagc ggctcgcttc aataactatt cagagggatt atggaaacac
     7201 gtcgcgaaga gcgtatcggg cagctgctgc aagaattaaa acgcagtgat aagttacatc
     7261 ttaaagacgc cgccgccctg cttggggttt cggagatgac gattcgtcgc gatctgaaca
     7321 accacagtgc gcccgtcgtt ttgctcggcg gctatattgt tctggaacca cgcagcgcca
     7381 gccattacct gttaagcgat caaaaatccc gcctggtgga agaaaaacgc cgggcggcaa
     7441 aactggctgc gacgctggta gaacccgatc agaccctctt ttttgactgt ggcaccacca
     7501 cgccgtggat tattgaagca attgataatg aaatcccttt taccgccgtt tgttattcgc
     7561 taaatacctt tctggcgctg aaagagaaac cccattgccg cgcgtttctt tgcggtggtg
     7621 aatttcacgc cagcaacgcc attttcaaac ccatcgattt tcagcaaacg ctgaataatt
     7681 tttgcccgga tatcgctttt tattccgctg cgggcgtgca tgtcagtaaa ggcgctacct
     7741 gctttaatct tgaagagttg ccggtaaaac actgggccat gtcgatggcg caaaagcatg
     7801 tgctggttgt cgaccacagt aaatttggca aggtgcgtcc ggcgcgcatg ggtgacctga
     7861 aacgctttga tattgttgtg agcgattgtt gcccggaaga tgagtatgtg aagtacgcgc
     7921 agacgcagcg tattaagttg atgtattaat gacgtataac cggatgacgt ttcgcgccat
     7981 ccggttatca gaagattaag agaaccagct gccgaaccac tgatggaatt tcatcatcac
     8041 gaaatcccac atccgaccaa agaatccgcc ctcttccaca ttttccatca cgatcagcgg
     8101 acgctgctca atggatttac cgttaagctg gaaatcaatg gtgccgacaa cctgaccttt
     8161 tttcagcggt gcggtaagct gcggttccgt taacgtataa ctcgccttca ggtttttcag
     8221 ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc ccgagattca cttcgctctt
     8281 atcaccaaac cagacgcgct gagtcacaaa tgtggcatca ggtttaattg gcgtcacggt
     8341 ttcaaagaaa cggaagcccc aggtcaataa tttctcagac tcattaaaac ggatacggtc
     8401 agttttcgcc cccagcacta cggagattaa acgcatatcg ccctgggtag ccgaagcaac
     8461 cagattatat cccgcgcctg ccgtggttcc tgtcttcatg ccatcaacat tcagattgct
     8521 gctccacagc agacgattac ggttaggctg acgaattttg ttgaaggtga actctttctc
     8581 tttatgaatg gcgtactctt ccggcacatc gtggatcaat gctttaccca gcaatgccat
     8641 atcgcgcgcg gtactgaact gccccggcgc atccagaccg tgcaccgtct ggaaggtagt
     8701 gttggtcaga cccagttttt tggcataacc attcatcaga ccaataaatg actcctggct
     8761 cccggcaacg taatcagcca gcgcaataca ggcgtcatta ccggactgga taatcacacc
     8821 tttgttcaag tctgccaccg aaacctgatc gcccggtttg aggaacatta ccgatgaacc
     8881 acgcagtgcc ggatttcccg tcgcccaggc atctttaccg accgtcacca tatcggtgag
     8941 tttaatctta tcggccttaa gcgcctgccc aaccacatag ctggtcatga ttttagtcag
     9001 gctcgcgggg tccagttttt catccgcatt gccttctgcc agcactttac cgctggcgta
     9061 atccattaaa atccatgcac gcgcatccac gctcggcgct tcaacggttt gttccgccgc
     9121 gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc gctgcaagac cacgaaggag
     9181 agaggagtat tgcgtcataa atgccacccg agtatccatt caaaaaaaat acgttatatc
     9241 gccgtatgat aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg
     9301 gtaaagtttt taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt
     9361 tgccagctgt tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagcctgtgg
     9421 ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaaccaac
     9481 gtaaaaaaag tattgctgac gctcgaagaa ctggaactac cttatgagca aattctcgcg
     9541 ggccgtgagt ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg
     9601 gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc
     9661 cgctatctgg cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt
     9721 gcggaagccg aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg
     9781 atcctgatgg gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc
     9841 agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa
     9901 tggttctcag gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat
     9961 ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa
    10021 ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg
    10081 ctaactttaa gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt
    10141 ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc
    10201 actttgtcgc cgttgacgct catcacaatg acatctttat ctttcagcgc gccaataaat
    10261 aatttttgct gccactgggg gaatttgtcg ctgttataga aggccatgcc gctcacggcg
    10321 ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgcc
    10381 tccggtatct taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt
    10441 ttgccttttt gcgggatatt aatttcatcg ccaccgcgcg ggccatgttc attcagccat
    10501 agtgcattac tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag
    10561 atctcggcgc gtgcatcgga ttcctttata aaaggattat catccgggat ttctcccagg
    10621 tcggtcagtc gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga
    10681 ttgttttcgc ccagagcaat aaaaagataa cctttaccgt caaataccat ccgcccgcca
    10741 aaatggttgc cggtagacag ttttggcatc tgacgaaaga cggtgcggaa gtcggtcact
    10801 tttgagagat catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg
    10861 ccaacttcgg aataacttaa ccagatgcgg cgcgactgag caaaatcggg cgctaaaacc
    10921 acgtccagca ggccgccctg cccgtgcgcc caaacgtccg gcactccgga aagcggcgca
    10981 gataatcctt ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata
    11041 ccgtgattgt cgggtaaaaa ggccagcgcc cagggatggt cgagtttgtc ctgcagtact
    11101 tcgacattta ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca
    11161 aggaaaaagg attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca
    11221 gtggaggaga taacaaattt atttttacaa aaacttaaac atgaaggggg agacgctttc
    11281 tcccccttag ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca
    11341 gcgttaagct catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc
    11401 aatcatggtg cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat
    11461 gaagagcact ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca
    11521 gttcgcgatt cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt
    11581 atccttttgc cttcctcagt tgcacgtatg cggcgaggtc aacgcgtgcg tcgatcaacc
    11641 ggttcagcag atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc
    11701 cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa tgctgtctag gatcaattct
    11761 tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat
    11821 acaaatttta tccagaactc tgtagaatcc ctgccctgac ctttactgta actgaatatt
    11881 ttttaagcta tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgc
    11941 ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac tacgcactga aggttatgac
    12001 gtggtaccga gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac
    12061 agcgcggtac aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta
    12121 attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg
    12181 ctggaaatca ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg
    12241 ccaaaaccga aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg
    12301 ccgcgtcatt atgcctatct gaaaatttct gaaggctgta atcaccgctg caccttctgc
    12361 attattccgt ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa
    12421 gcgaaacgtc tggttgatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc
    12481 gcctatggcg tcgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc
    12541 agcatggtca gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac
    12601 gtttaccctt atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg
    12661 ccctatctgg acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt
    12721 ccgggttctg tagatcgcca actcgcgcgc atcaaacagt ggcgcgaaat ctgcccggaa
    12781 ctgaccctac gctcaacctt tattgtcggc ttccctggcg agacggaaga agatttccag
    12841 atgctactcg acttcctgaa agaagcacgt ctggatcgcg ttggctgctt taaatacagc
    12901 ccggttgaag gtgcagacgc caatgccctg cctgaccagg ttccggaaga agtgaaagaa
    12961 gaacgctgga accgtttcat gcagttgcag cagcagattt ccgccgagcg cctgcaagag
    13021 aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg acgaagaagg tgcgattggt
    13081 cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac
    13141 gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg
    13201 ggtagccggg tttaattaat cttaaggcag atctaacaag gatattgttc ttcgatctgc
    13261 ctctgctacc cttcccgatg tcctaccctt acgcacaaaa tcccctgacg cataaatcga
    13321 gaaaaatgaa tcgtactcga ggcaactctc actttttcac accaaattaa agttttgatc
    13381 gcaattactt gaaataagcg taatggttac tatagtaaac attaccacaa ggatggatgt
    13441 atgggtgtgt ataaggcaag acgattcagc caaagcacga aaaaacttgg tattcacgat
    13501 aaagttctca tggctgccgc tgaagaagtg attcagggca tatgggaggc tgatttaggt
    13561 agcggtgtca tcaaaaaacg tttacccctc caacaaggaa agagtggtgg agccagaaca
    13621 attatctttt ttaaatcagc aaatcatgtg tttttttatg atggttggtc caagagtggt
    13681 ctaagcagta aaggaaccaa agaaatcgaa gatgatgaac tggcggccta caaaaaaatg
    13741 gcaaatgcat ttctggcctt tagcaataag caaatagagg atttgatcga aactggcttt
    13801 ttgattgagg tgaaaaatga gcgataaatt acaaatgatg ctggaagatg caaaggaact
    13861 gaatgcactg ggcctgatgt ctgatagtga agttgatgcc attgccgtca tggtcaaagc
    13921 tcgtgaagtc agcgcccgta tagccaaagt caaatcaatg acgggtgaag agatcaaagc
    13981 catccgcacc cgatacggca tgtcacaatc cgtgctggca catacaatgg gaatgtcaaa
    14041 agaaagtgtc tcgaaatggg aacgtaatga aataaaaccg agcggcccgg cattacgtat
    14101 tttgaataca ttggcaataa aagggccgga agtttttgca aagtaaaatg tttgcaaatg
    14161 atttaccaca ttcattagta ccccttgatt aaggggtact aacactattc taattctact
    14221 gccagctaat ctgcgccctg ccgtttttct tactcttata cagtcgctca tccgccagca
    14281 ttttcaagcg ttcgacggta gtacattcat ttgaatgcgc tacaccgata ctggtgctta
    14341 aattaatgct tcccacctgg aaatgaacaa tttcttttag aatcgatagc aactcttcca
    14401 gcgttccacc cgtataaatc accgcaaatt cgtcaccgcc gaagcggtag actttacctt
    14461 tatctacggg gagctgtgat ttaaggattt tcaccacttt catcaacact tcatcgccca
    14521 gcaaatgacc gaaggtgtcg ttaatgtttt tgaatttatc gacgtcaaac aggcacaaat
    14581 aaccgccttt ttccggcagc gcttttaaat cttcgtcaaa ggattttctg cccatcgctt
    14641 tggtcagatc gtccaggaat gccgagttat acatactgta gagttttttg acatcggcag
    14701 ccgaaaagaa aagcccgcca tccagtacgc gttcaaaatg gttattaata atggcttctt
    14761 tctccacaat accacgatta atcactctgc gtaccagata gaggcaactg gcacccgata
    14821 tcactgccac caatgcggca aagaaaataa tcaatgtaca tacctgaaaa taaacgtctt
    14881 ttttataagc aaatacgtac aacccggtcg gcaaataggc gaagtgctcc atcgcttccg
    14941 aaacagtgaa gatggcgttt ttagcgttat attcatcacc ggtcaccgat ttaccgttct
    15001 tgaacaggga gaacatcagg ttctcgctat ccatcgccag cgattttgac agtaaagaaa
    15061 aacgttccag accaagcaaa aatatcagac tacgattgtg agttaccgga tagaggtaat
    15121 agacgaaggt tttaccatca aaagagataa accctgtatt tttgacatgt cgatacttca
    15181 caacctgcat aatatctgca caataatttt tctcgaactc ctttacctga atacattcgt
    15241 cactggaaat cagataagga ccgacatcgt acaaataaga gggtgaaacc gagtcggcgt
    15301 aagctgtgcg taagccaacc aacagctgtt cctccgtggg atttagcgtg ttgataagat
    15361 caattgccac gctacgccga tattcagcaa tatttgccac cgcaaaagca gtatcaataa
    15421 cggactgttt caggctatac acccgttcct ttaccatttg agtgtagatc ccgcaggcaa
    15481 ctgctgaaat catgataaaa atcagcagac tgactgtaag tacgaactta ttgattctgg
    15541 acatacgtaa actactcttt tactaatttt ccacttttat cccaggcgga gaatggcatg
    15601 ggtttctggt acagataccc ctgagcctga acacatccta atttgattaa ttcgcgcgca
    15661 acgtcttcgc tctctacgcc ttccgcaatc accgggatcc ggttatagcg tgccagatcg
    15721 aaaataatgc gcacaatttt tcccgcagta ccaaaaatat cgccggtaaa ctgcgagtcc
    15781 acctttatgc tgtctggatt aatttcataa aaacgtttca ggtttgacaa accggtgcca
    15841 aagtcatcaa tcgatatccg taccccttgt ttacgcagtt gttcaatatt gagataaaaa
    15901 tgttcattaa ttcgctcaat cacggtttca gtgatctcaa cacaaaggct gttcgccttc
    15961 aggccatttt ctttcatgat ggtagtaaaa cgctcatgaa agtgtggttc gtttaactgc
    16021 cagggcgaaa cattaatatg cagtaagaaa tcatccttca gcgacgcacg ctgactaaaa
    16081 tgtctgaatt cacccatcgc ggttttaata atttgatagc ccagctcatt gataaacccg
    16141 atatcttcag caatggtaat gaacttcagc ggtgaaatga tcccccgctc cgcagacacc
    16201 caacggcaca acgcctccgc gcccagaatg gcgcgggtgt taatatcaac gatcggttga
    16261 tagacgagga atagttcgcc aatttccatt gcatggcgaa tattacgcgc aatccagata
    16321 ttctcttcta tttcgttgag tttcgcatca tcacagaaaa taaactctga gaacatatgg
    16381 ttgcggatac tggaaatcgc cagtttcagc ttttctgcgt aactccaggc gttttccccc
    16441 tgcaaaggtt cacaaatagc agacttatgg atgcgcagat tgcggtagcc aaaactgctg
    16501 tcaaaatcgt ttaccgtcgc tatcttacgg gtaaacgtct gtacattttc ccggcacagg
    16561 agtaaataga gatcggcatt aaatgtcacg atatcgcagc attgcgcata tttctcggca
    16621 attcgctgac acaatttcct gacgatttta ttgcgttcat caacaccaaa caccgcttcc
    16681 acagcttcca gaccacacag ttagatgatc cccacagcca gatactgatt attgttggat
    16741 tcaataatcg ccatattttt aataaaaccg ctttcctgat cgtaagagat cagtgagagc
    16801 aatttatttg accgttcaat ttcggcatct ttcatttccg cgatcgaatt tttgatgctg
    16861 ataatctctt tcgcatcgcc ttcgcgaatt ttcagagcct cgatctgatc cgttgagtcg
    16921 ggcaaaaacg ccagcgtatt aaccagctcg gtcatttcag aaaattgccg acggaacaca
    16981 cgagtggtca ttaaataaac caccaacaat acgataataa tgaacgcaaa cggcagataa
    17041 aaaagtttat ctgatgcgtt caataaatta ccaccaggat aataaaaata gatttcgaat
    17101 aatgcgttgt ttggcaccg
//
LOCUS       DAZGAP010000118        17115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGAP010000118 DAZGAP010000000
VERSION     DAZGAP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.119"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..381
                     /gene="bssS"
                     /locus_tag="ACHLAM_003350"
     CDS             127..381
                     /gene="bssS"
                     /locus_tag="ACHLAM_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO7854825.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            496..1614
                     /gene="solA"
                     /locus_tag="ACHLAM_003351"
     CDS             496..1614
                     /gene="solA"
                     /locus_tag="ACHLAM_003351"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO7854826.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1662..1775
                     /locus_tag="ACHLAM_003352"
     CDS             1662..1775
                     /locus_tag="ACHLAM_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO7854827.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
     gene            2036..2602
                     /gene="yceJ"
                     /locus_tag="ACHLAM_003353"
     CDS             2036..2602
                     /gene="yceJ"
                     /locus_tag="ACHLAM_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO7854828.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2606..3181
                     /gene="yceI"
                     /locus_tag="ACHLAM_003354"
     CDS             2606..3181
                     /gene="yceI"
                     /locus_tag="ACHLAM_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO7854829.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3223..4275)
                     /gene="trhO"
                     /locus_tag="ACHLAM_003355"
     CDS             complement(3223..4275)
                     /gene="trhO"
                     /locus_tag="ACHLAM_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO7854830.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4500..5420
                     /gene="lpxL"
                     /locus_tag="ACHLAM_003356"
     CDS             4500..5420
                     /gene="lpxL"
                     /locus_tag="ACHLAM_003356"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO7854831.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKTLKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5592..6818
                     /gene="mdtG"
                     /locus_tag="ACHLAM_003357"
     CDS             5592..6818
                     /gene="mdtG"
                     /locus_tag="ACHLAM_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO7854832.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6901..7275
                     /gene="msyB"
                     /locus_tag="ACHLAM_003358"
     CDS             6901..7275
                     /gene="msyB"
                     /locus_tag="ACHLAM_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7854833.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7276..7503)
                     /gene="yceK"
                     /locus_tag="ACHLAM_003359"
     CDS             complement(7276..7503)
                     /gene="yceK"
                     /locus_tag="ACHLAM_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7854834.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7675..10218)
                     /gene="mdoH"
                     /locus_tag="ACHLAM_003360"
     CDS             complement(7675..10218)
                     /gene="mdoH"
                     /locus_tag="ACHLAM_003360"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO7854835.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10211..11746)
                     /gene="mdoG"
                     /locus_tag="ACHLAM_003361"
     CDS             complement(10211..11746)
                     /gene="mdoG"
                     /locus_tag="ACHLAM_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO7854836.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12140..13297
                     /gene="mdoC"
                     /locus_tag="ACHLAM_003362"
     CDS             12140..13297
                     /gene="mdoC"
                     /locus_tag="ACHLAM_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO7854837.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13305..14786)
                     /gene="clsC"
                     /locus_tag="ACHLAM_003363"
     CDS             complement(13305..14786)
                     /gene="clsC"
                     /locus_tag="ACHLAM_003363"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO7854838.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGIFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14728..15261)
                     /gene="ymdB"
                     /locus_tag="ACHLAM_003364"
     CDS             complement(14728..15261)
                     /gene="ymdB"
                     /locus_tag="ACHLAM_003364"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO7854839.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15356..15667)
                     /gene="ymdA"
                     /locus_tag="ACHLAM_003365"
     CDS             complement(15356..15667)
                     /gene="ymdA"
                     /locus_tag="ACHLAM_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO7854840.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15788..16120)
                     /gene="csgC"
                     /locus_tag="ACHLAM_003366"
     CDS             complement(15788..16120)
                     /gene="csgC"
                     /locus_tag="ACHLAM_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO7854841.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16179..16634)
                     /gene="csgA"
                     /locus_tag="ACHLAM_003367"
     CDS             complement(16179..16634)
                     /gene="csgA"
                     /locus_tag="ACHLAM_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO7854842.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16675..>17115)
                     /gene="csgB"
                     /locus_tag="ACHLAM_003368"
     CDS             complement(16675..>17115)
                     /gene="csgB"
                     /locus_tag="ACHLAM_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO7854843.1"
                     /translation="LFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQAAII
                     GQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQG
                     AYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4279 a         4369 c         4076 g         4391 t
ORIGIN      
        1 tttatctgtt ccccccttta aaaattcggt gaataccctt acttattggt atactgaagt
       61 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
      121 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
      181 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
      241 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
      301 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta
      361 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
      421 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
      481 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
      541 gccgccgggt attatgcaac ccgtgccggt ttaaacgtgc taatgaccga cgcccatatg
      601 ccaccgcatc aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt
      661 gaaggcgaaa agtatgtccc gctggttctc cgcgcgcaaa cgctgtggga tgaactctcc
      721 cgccacaacg aagaagatcc catttttgta cgctctggcg tcattaacct tggcccggct
      781 gattccacat ttctcgccaa cgtcgcccac agcgccgaac aatggcaact caacgttgaa
      841 aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
      901 atcggcttat ttgagactga ttccggcttt ttacgcagcg aactggcgat taaaacctgg
      961 atccaactgg cgaaggaagc gggctgtgcg cagctgttca actgcccggt caccgcaatt
     1021 cgtcatgacg atgatggcgt aactattgaa acggctgacg gtgagtatca ggcgaaaaaa
     1081 gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagttgcc tgtccagccc
     1141 gtacgcaaag tatttgcctg gtatcaggcc gatgggcgct atagcgtgaa gaataaattc
     1201 ccggcgttta ccggtgaact tcccaatggc gatcaatatt atggttttcc ggcagaaaac
     1261 gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
     1321 gttccgtttg cggaagtggt cagcgatggt tcggaagcct tcccgttctt gcgcaatgta
     1381 ttgccgggta tcggttgctg cctgtacggc gccgcctgca cctatgataa ttcgcctgac
     1441 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
     1501 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
     1561 gacaaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca
     1621 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact
     1681 atctcatcaa taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg
     1741 caattatgga tctgatctat gtgtattatt tttagttttc cgcactgaca taaactgaca
     1801 aatatttaat ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttcaac
     1861 agcactcacc agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat
     1921 taattcaaaa ttattgaaat aaaaataaat atcattcaaa ttgttatttt tgtgttgcaa
     1981 ttcattctcc gacacgccat tttctttctc tcgcaaagtc gttttggatg cacttatgtc
     2041 attcacaaat acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc
     2101 aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga
     2161 tggctggtat caccaggcac ccgaactgca taaaagcatc ggtattttgt taatgatggg
     2221 gctggttatt cgcgttctgt ggcgcgtcat atctccaccg ccgggtccgc tgcagagtta
     2281 ttcgcccatg actcgtcttg gcgcaaaggc tggacatctc gcattgtact tattgctgtt
     2341 cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt
     2401 tggctggttt gacgtccccg cgaccctcgc cgacgctggc gcacaggctg actttgccgg
     2461 tgccctgcat ttttggctcg cgtggagcgt cgtagtactg tccgttatgc acggatttat
     2521 ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc
     2581 gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcttc
     2641 cctgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca
     2701 gcacgccttt gttaacttcc gcatacagca ccttggctat agctggttat acggcacctt
     2761 taaagatttc gacggtactt ttacctttga tgaaaaaaat ccagctgccg ataaagtgaa
     2821 tgtgacaatt aacaccacca gcgtcgatac taatcacgcc gaacgcgata aacatcttcg
     2881 cagtgcagat ttcctcaata ctgcaaaata tccacaggca acattcacct ccaccagcgt
     2941 gaagaaagac ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa
     3001 acccgtcacg ctggaagcga aattaattgg tcagggcgac gacccatggg gtggtaaacg
     3061 tgcaggcttc gaggccgaag gcaaaattaa gctcaaggac ttcaatatca agacagattt
     3121 aggtccagct tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta
     3181 agagtgaaat gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg
     3241 aatgcccagt gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc
     3301 acgtcctgcc cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat
     3361 ttcactacaa caacctttgt atttttctgc acatactgga cactgaataa acagcaggtg
     3421 gcagccatca tttttacagt tggtatggct gtcgcacggc gcaccgcact ggtggcaatg
     3481 cgcgataatc tcatcagata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc
     3541 aataaaacgt accggtaaac cctgctcgcg cgccttgcgg gcgtattcaa taattccgcc
     3601 ctcgatatgc cagactttat tgaatccgtt atgtttcatc caggcactgg ccttttcaca
     3661 acgaatgccg ccggtgcagt acataacgat ttttttatct ttatgtgcct gcatcatctc
     3721 tactgctttt ggcagctgct cacggaaggt atctgccgga atttccagcg cgttttcaaa
     3781 gtgccccact tcatactcat agtggttacg catgtcgata aacaatgcat cgggatcgtc
     3841 aagcatggcg ttcacttccg ccgcttgcag atactcaccc acattgctgg catcaaagtg
     3901 aggatcgtca ataccgtcgg caacgatacg atcgcggacc ttcatgcgca gtacccagaa
     3961 ggatttcccg tcatcatcca acgcgatatt caggcgtaag ccctctaaag ccgggtcgaa
     4021 ggcataaagc tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt
     4081 aatgccctca tgcgccagat acactcgccc aaaaacatcc agcgcggtaa acagctgata
     4141 taaagcgtca cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt
     4201 ggttcgcggt tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt
     4261 gtgtaacact ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg
     4321 atatagcaat tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa
     4381 ttatttgtta tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc
     4441 gggcgaaaaa atgcgacaat acatacaatt gcccgtatag gttgaaaaac aggattgata
     4501 tgacgaatct acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt
     4561 tgggtattgg cgtactttgg ttagtcgtgc aattgcccta cccggttatc taccgcctcg
     4621 gttgtggatt aggaaaactt gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc
     4681 gcaacctgga actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga
     4741 agaatttcga atccgttggc atgggcctga tggaaaccgg catggcgtgg ttctggccgg
     4801 accgcagaat cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg
     4861 cgcaaaaacg cggcatcctg ttagttggca tccattttct gacactggag cttggtgcgc
     4921 ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga
     4981 ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag
     5041 atttaaaagg catgattaaa accctgaaaa aaggcgaagt ggtctggtac gcaccggatc
     5101 atgattacgg cccgcgctca agcgtttttg tcccgttgtt tgccgttgag caggctgcga
     5161 ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc
     5221 cacgccgtaa gccagatggc aaagggtatc agttgattat gctgccgcca gagtgttctc
     5281 cgccgctgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca
     5341 tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgtccggaag
     5401 gcgttccttc acgctattaa atctcccaag ccggatgctt gagaatagca tccggcatta
     5461 ccacagcaaa tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc
     5521 gggcgcataa ttagtgtgct tatcttttct tcttatgttc accgcgcctg gcgcaccaac
     5581 agcggattgc tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga
     5641 tcgtcgcctg gctaggctgt tttcttacgg gtgccgcctt cagcctggta atgcccttct
     5701 tacccctcta cgttgagcag cttggcgtta cgggccactc cgccttgaat atgtggtccg
     5761 gtattgtctt cagcattaca tttttatttt cggccatcgc ctcaccgttt tggggtggac
     5821 tcgccgaccg taaaggccga aaactcatgc tattacgctc tgccctcggc atgggcatcg
     5881 tgatggtgtt gatgggactg gcacaaaata tctggcagtt tttgatcctg cgggcgcttc
     5941 ttgggttact tggcggattt gtccccaacg ctaatgctct tatcgccaca caagtaccgc
     6001 gtaataaaag cggctgggcg ctgggtacgc tctccacagg cggcgttagt ggtgcgttgc
     6061 tcggcccaat ggctggcggc ctgctcgccg atagctacgg cttacgtccg gtattcttta
     6121 ttaccgccag tgtgctcata ctctgctttt tcgtcaccct gttctgcatc agagaaaaat
     6181 tccagccggt cagcaaaaaa gagatgctgc acatgcggga agtggtgaca tcacttaaaa
     6241 acccgaaact ggtactcagc ctgtttgtca ccacgttaat catccaggtg gcgacaggct
     6301 caattgcccc cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg
     6361 cctttatcag tggcatgatc gcctcggtgc caggcgtggc ggctctgcta agcgcaccac
     6421 gactcggcaa acttggcgat cgaatcggac ccgaaaagat cctgattaca gcgctgatct
     6481 tttctgtact gctgttgatc ccaatgtctt acgttcagac gccattgcaa cttgggattt
     6541 tacgtttttt gctcggtgcc gccgatggtg cactactccc cgccgtacag acactgttgg
     6601 tttacaactc gagcaaccag atcgccgggc gtatcttcag ctataaccaa tcgtttcgtg
     6661 atattggcaa cgttaccgga ccattgatgg gagcagcgat ttcagcgaac tacggtttca
     6721 gagcggtatt tctcgtcacc gctggcgtcg tgttattcaa cgcagtctat tcatggaaca
     6781 gtctacgtcg tcgtcgaata ccccaggtat cgaactgatt tttcgccttt catacttgca
     6841 aaagcggaga atcagctatc cttttccctg aaacctcatc aactcaaagg gagaatcgtg
     6901 atgaccatgt acgcaacgct tgaagaagcc attgacgctg cacgcgaaga atttcttgca
     6961 gacaaccccg gcatcgacgc cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac
     7021 gttttgcagg acggcgacat catgtggcaa gttgagtttt ttgccgacga aggggaagaa
     7081 ggtgaatgtt tacctatgct tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat
     7141 gatgagatag agatacgcca ggagtggcag gaagagaata cattacatga atgggacgag
     7201 ggggaatttc agcttgagcc accgctggat accgaggaag gacgcgcagc agctgatgag
     7261 tgggatgaac gttaatcact catacgggcc atgatgaatg tcgatcggca gcagtaaagt
     7321 atcgaaaacc agggagaatg gcagatcaag gatcgtgacg taacgccagg cggagtcacg
     7381 cacgtcccat tgcacaccgg gataatattg gttgccatgc ccctgccccg gtatagtgcg
     7441 actaatgatg ctgccacagc cactcagcaa ggtcaccatt atgctcatca caattaaacg
     7501 catttgatac ctcttccgta aaaaataccg gcataacgcc ggtattttta ttggctcgtt
     7561 gtttttgccg gatgcggcgt gaacgcttta cccgacctac aaaatcttgc aaaatcaata
     7621 aattgcagga acgatgtagg cctgataagc gtagcgcatc aggcaactac gtttttattg
     7681 cgaagccgca tccggtttac gcaatgccag tggattgagc tttatccctt cgtaataact
     7741 cacccatgaa gaatatctct ccggggaatt ccagacgcgg aaatgcagac gggccatcgt
     7801 caccggatcg cttagcagca ccaggcgacg atcgcgattc agcttctctg gcgtctcgtt
     7861 cagcgcctgt tcaacgtggc ggtcacgggc gatttccagc accttgctgg cgcggtgacg
     7921 cgcggtcgcc attgcggttg ccagagcgtt aaatgacggg ttaaacaccg cgtgcataaa
     7981 accatcatca agggagcgtt gacgattcat ctcaaggaac cgatcggtat caaccagcac
     8041 ctgcggcggt gaatactctt ccgggatcag gaacagtttc cagcgtttgg ttcgcagacc
     8101 aacggtggca cggctggaaa tcaccgaaac aaacggtgac aggatcaacg agaagacaat
     8161 cggtgccagc cagaacagaa aacgcagatc caaccacgcc atcccaacag cccacactaa
     8221 acccaacagc agctgtgagc cgtggcgttt gaatgcttcg ccccaggaag tggagtcatc
     8281 atcacgctgc ggtgaattcc acaccacttc ccagccaagg aacgcgctga cgacgaagac
     8341 tgtatggaac aacatgcgta ccggagccag caacacggaa aacagcactt ccagcagcaa
     8401 cgataatgta acgcgccaga agccgccgta ttctttcgtt cctttgcacc agataagcaa
     8461 aatgctcaat agcttcggca ggaacaacag caccatggtc gaagcaaaaa gtgcaatcgc
     8521 cagctcagga cgccactgcg gccataccgg gaacaactgc cgtggttgca ggaagtattg
     8581 cggttcggtc aacgcatgta ctacctgcaa tgcagtagag agcgcgagga acataaacca
     8641 cagcggagcg gagagataag acatcacgcc cgtcaggaac accgcacggt gaaccgggtg
     8701 catacccttc accaggaaca gacggaagtt catcaggtta ccgtggcacc agcggcggtc
     8761 acgttttagc tcatcaagca agttaggcgg caattcttca taagaacccg ggagatcgta
     8821 agcaatccac accccccaac ctgcacggcg catcaacgcc gcttctacga agtcatgtga
     8881 caggattgaa ccggcaaaag aaccttcgcc cggcagcgga gccagtgcac aatgctcgat
     8941 aaacggtttc acgcggataa tcgcgttatg cccccagtag tgcgactcgc caagttgcca
     9001 gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg gtcgcgaact gctgacagcg
     9061 cgcatacagc gtatccatac cggacgcttt cggcgacgac tgaatgatcc cggcgttcgg
     9121 gttggcttcc atcaagcgca ccaggccgca taaacaatca ccggtcatta ccgagtcagc
     9181 atccagcacc accatgtagc tgtactggct gccccagcga cggcagaagt catcgatatt
     9241 accgctttta cgcttcacgc gacggcggcg gcggcgatag aaaatctgac cttcgccacc
     9301 gacttcagcg ataagctcca tccaggcttt ttgctctgcg acgcagatat ccgggttata
     9361 actgtcactc agaatgtaga catcaaagtg tttggcattc ccggtggctt ttactgattc
     9421 ccacgttgca cgcaagccag caaaaacacg gttcacgtct tcgttacaga taggcatgat
     9481 caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca actgttgacg cggatatact
     9541 gtatttatcg cgaccaataa gcagttgcag gaagcccatc aacgccgtcc agaatccggc
     9601 ggacacccaa cagaacagta ccgcaaagag gatcaggata ccggtttgca gcatataagg
     9661 cagaagctgc ataaaagaaa cccacacatc ctgaccaacc atatccatag gattaatcag
     9721 cgcccacccc tgataaggaa gaatggtctt catataccag gtcgcgacaa cagtttgcgc
     9781 gagcgtcagg atcaacagaa tgtaacggcg gatggtaccg acggtacgcc acttttgctc
     9841 gctctcctgc tcttctttgg tcaaacgagc cagatagcgc ggcgtcacat cgcgtccacg
     9901 caggcgatcc cagaaacggc ctaccgggtt ggtacgccac gggtcgggaa acatcgagga
     9961 gcgttttgct tctggcatcg ccttaagctg atcgcgccct tcgtcatctt taattaactg
    10021 tccatcagca agtgaatctg gccaggcttg ttccagacgc gcctttaccg agccttgcgg
    10081 ggagtcatcc tcccgcgccc aggtgcggtg ttcggcatcc agcgcctgat gaacggcgcg
    10141 gatatcagtc ttcggcaatg ccgctttctc gcttgcggcg atgggcattg cgtcaatgta
    10201 ctcagttgtc ttattcattg gcaggtaact ggtagctcca ggtttcactc aacgtctgat
    10261 cggcattcac cagcgcagca cgcatttcag tggttttctt ggcatctttc actttcacac
    10321 gcatcaccag acgccagcct ttggtaaccg ggttatagcg caccgtgctt tcaactatct
    10381 caccattatc accaatgctg gtttgcgctg tgaccggggt atcctctggc agttttttca
    10441 tctctgcgcc ggtaaaatcg accacaaagg cgatagtacc gtcaggttgg cgaatcaggt
    10501 tcgactgctt cacatccccc gttgaacgac gcgtttgttg cacccatgcg ttatctggcg
    10561 catgcagttt gtcttcatca cggctgaagg tgatggtgta tttaaagttc atctctttac
    10621 ccggctccgg cagctgatcc ggcgtccagt aagcgacgat gttatcgttg gtttcatcgt
    10681 tggttggaat ttccaccagc tcaacgctgc ctttgcccca ctcccctttc ggagtcaccc
    10741 atgcgcttgg acggagatcg taacgatcat cgagatcttc aaagcgggag aaatcacgac
    10801 cgcgctgcaa cagaccgaag ccttgcgggt tttccatcga gaagctgctg accgctaaat
    10861 gtttcgggtt attcaacgga cgccagatcc attcgccatt accagcatgg atagagagac
    10921 cgttagagtc gtgcaactcc ggacgatagt tatttgcagg cgacggttgg ttcggcccaa
    10981 acaggaacat actggttaac ggtgcaaccc ccagtttgcc gactttatcg cgcagataga
    11041 ttttcgactg cacatccaca accgtgtcac gccctggcat aactacgaat ttgtaagcac
    11101 ctgtcgcgcg cggcgagtca agcaatgcat aaatggttaa acgtttatca gtcggttttg
    11161 gacgctcgat ccagaactct ttgaagcgtg gaaattcttc acccgatggc aaggcggtat
    11221 caattgccag gccgcgtgca gaaaggccat aaacctgacc tgcaccaatc acgcggaaat
    11281 agctggcccc gagcatgctg acgatttcat cgtttttatc tttgctgttg atcgggtaaa
    11341 gcactttaaa accggcaaaa ccaaggtctt ttaccgtgtc tttgtcatgc tgaacatcgc
    11401 cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac tgcggtggca gtcacttcat
    11461 ttattttgac cggggtatcg aagtacatac cctgatggta gaactcgagt ttgaatgggg
    11521 tcttcagatt gttccagtac gctttgtcat gattaaactg gatctgctga tagtccgcgt
    11581 atttcatgtc gcggaaaacg gagggcaagt tgcttttagg cgcctcatag cctttcccgg
    11641 ctaaggattg agcttgcttt gcgacatcat caatactgaa agcccagctt gaagatgtat
    11701 acagggttaa cattactgca gcactcaacc aacgcatttt catcatttgt agtttatgtt
    11761 tcataattag taagcacttc cccctttgtg tgcttatatc gatccgatct attttaatgg
    11821 ataaccagcc attccgacaa cagaatcccg gtatcgttca gcacgctggc tcatggatta
    11881 agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc taaagacaga agcatcgact
    11941 tcgtgtaggg tttgccagca aatttattta tcaaccgtct tacagacgct tccattctgg
    12001 ttagtggtag cgatgatgct atgcataaaa accttgaagc ctgcgcctgg cgcggtcagg
    12061 tcggtgtcgc tatggcaaca agcctaaagg cataacccga cagggataag acgaaaaatc
    12121 cgagattagt taaccatata tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc
    12181 catccgcgcc tggctgatgt tgttagggat cccttttcat atttctttaa tctattcgag
    12241 ccatacatgg catgtgaata gcgccgaacc gtcattgtgg ctgacccttt ttaatgactt
    12301 catccactcg ttccgcatgc aggtattttt cgttatatcc gggtactttt cctacatgct
    12361 ttttttacgc tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg taggtatccc
    12421 gatgttaaca gccatccccc tactgacatt accgcaattt attatgctgc aatacgtcaa
    12481 agggaaagcg gaaagttggc ctgggctatc attgtatgac aaatataata cgttggcctg
    12541 ggaattaata tcacacctgt ggtttttact ggtgttagta gtcatgacga cgctgtgcgt
    12601 atggatattt aaacgcatca gaaataattt agaaaattct gataaaacga ataaaaaatt
    12661 ctcgatggta aaactatcgg tgattttttt gtgcctcggc atcggttatg cggtaataag
    12721 aagaacgatt tttattgtgt atccgcccat tctgagtaat ggcatgttca attttattgt
    12781 catgcaaacg ctattttatt tgccgttctt tatcctcggc gcactggctt tcattttccc
    12841 tcatcttaaa gccttgttta ccacgccgtc tcgtggctgt acccttgcag cagcattggc
    12901 atttgtcgct tatttactca accagcgcta tggcagtggc gatgcctgga tgtacgaaac
    12961 cgagtcggtg atcaccatgg tcctcggtct gtggatggtg aatgtggtct tctcctttgg
    13021 ccaccgtttg cttaacttcc agtcagcgcg ggtgacttat tttgttaacg catcgctgtt
    13081 tatctatctg gttcaccacc cgttaacgct gtttttcggc gcatacatta caccgcacat
    13141 cacctccaac tggcttggtt ttctctgtgg cctgatattc gtagtaggga ttgcgataat
    13201 tctgtatgaa attcatttac gcatcccgtt actgaagttt ttgttctctg gtaaaccggt
    13261 tgtcaagcgt gagaacgata aagcaccagc ccgttaagcc acgcttataa taaccattcc
    13321 acgggcaata tcgacgccag tctgaccata acccgcttcc agaaactggt ggcgggttct
    13381 tttttgagaa taatctcttt ctttgcatga cgatcaaccc agttgatccg tccccacctg
    13441 tccagacgga gctgccaggc cgcatcatac tggctctgaa taaagcgttt atcaattaac
    13501 tgtgccagcg tttcgctctc tatcacgaag cccatttcag tattgagcaa tgttgaacgc
    13561 ggatcgaaat tgaaagagcc gataaacacc gttttaccat cgatgctaaa ggttttagca
    13621 tgcaagctgg caccggaatt accggttatg ccgcgatcgt gtaacgtact actttgttca
    13681 cgcgtcggct tgagttcata taattccacg ccatagcgga gcaatttttt gcgccagcgc
    13741 gcgtatccgg catggacgac agcaacatcg ttagcggcaa gagaattggt taggatcgca
    13801 atctttaccc cttttctcac catccgtaag agttgcgcca cacctgcgcg tgtcggtaca
    13861 aaataggaag agataatatc gatgcgttca ctgggtgagc ccatgatatc gaacaggcgc
    13921 tgcggtaaca gtgaatgccg ttttgccttg ccttcccctt tcgccggatc atcacttaat
    13981 aaacgtgttt tcgcccagat aagcggcaac gttccatcaa ccagatgatt tataaacggg
    14041 ctggattcca ttttgcgtaa ataacgatgc gtcatggcat cgttgtgcca ggatgctggt
    14101 aactcgatgc gatccgccat ttcaccttcc gggacatcca gcacctgctg taggggtgaa
    14161 accgatttgc aataccagta gcgggcgaaa tcatcggcaa cgtcctctac aactggtcct
    14221 attgccatga catctaaatc cgaaaaaagt ggctcttccc ctgccccaaa ataggcatca
    14281 ccaatatttc gcccccctac cagcgtcacc acgccatcga cagtgaaact tttattgtgc
    14341 atacggcgat tgagacggga aaagtcggtg atataaccaa gcggacgtag caagcgaaac
    14401 gagaaaggat taaaaagccg gacttcaatg cggggatgac tgtcaagcaa gcgtaaaatg
    14461 tcgtcaagtc cgggcgtatt gttgtcgtcc agcaacaaac ggacgcgaac gccacgcttt
    14521 gctgcggcta acagggcgga aaagagtaac cgacccgaca tatcgtcctg ccagatgtaa
    14581 tactgaacat ccagcgtatg ctctgccatt tcggccagac gataccgggc ggcaaacgca
    14641 tccagacttt tctccagagg aaaaatgcca cactgaccgg gatgttgcga acacagtggc
    14701 agcaccgcac tcgccagccg gggcaaatca ttcatctcct tgttgagtaa ggagtctttc
    14761 gtagaggtgg gcgttttctt catcataaca gacaaagtat acctgttcgg gtaaagcgtg
    14821 acgggtaata aattctgaaa cggtttttac tgcgatttca gccgctgccg cacgagggta
    14881 accataaact ccagtactga ttgcaggaaa agccactgac gtatagctgt ttgctgccac
    14941 cagtcgcagg ctattgagat aggcatcctg caaaagctgg tcttcgtttt gttcaccacc
    15001 acgccagact ggcccgacgg tgtgcactac ggctttcgcg ggaagatcgc ctgcaagcgt
    15061 aataacggca tgccccgtag ggcaatcgcc ctgctgctgc ctgactttta aacaggcatc
    15121 cagcagggcc ggacccgctg cgcgatgaat ggccccatcg acgccgccgc ctcccattaa
    15181 tgacggatta gccgcattca caatcacatc aacggccagt ttggtaatat caccctgcac
    15241 aacatgaata cgcgttttca taacgcctcc ttacaccttc ttgtttctta agtgtacagc
    15301 aggctttttg gttaaatcgc atttttctgc tcagcaacgt gagttgttca gaaacttagt
    15361 tatattcgat gttcattaca gccagctttt tctgtttatc aagatactgc acttttactg
    15421 aggctatttg cgcgtttttg acattgccgc tcattaacgc tttgctggaa taggttctgc
    15481 ccggaacaga gccattctgt gggcagttca tttctacggg tgatgactgg gtgctgacgt
    15541 cacatgccgg ttccacaatt tggccataaa aatggatcac gcccccttgc gcggtgcttc
    15601 ctgcgatggc aataaaagga aaaaacatac tgccgagcat tagagagtct aaaaaaggtc
    15661 tgaacattta ttctatttcc tcaatgatta gtcatccttg agggttgtgt tatccatact
    15721 ttcaggttgg ctgatccgca gcagaccatt ctctccagat tcatcttatg ctcgatattt
    15781 caacaaatta agacttttct gaagagggcg gccattgttg tgataaatga agtgactgtc
    15841 catcagaaac agtaacaact attttcaccc gatcgtccgg ggaaatattt aaactcaact
    15901 tcgtcaaagc aatgggttga ttagcaggca atgagagggt cttttcttgc ttcgtctgac
    15961 tttgccctga actgccttcg cgcagggaca atatttgtac tctgcacaga caagattgag
    16021 taagagtgac ttcaggaata atggtgtaca tatccccttg ctgggtcgta ttaaaggtta
    16081 tctgactgga aagtgccgca aggagtaata acgcattcat attcttctcc cgaaaaaaaa
    16141 cagggcttgc gccctgtttc tttaatacag aggatgtatt agtactgatg agcggtcgcg
    16201 ttgttaccaa agccaacctg agtgacgttg acgctggagt tagatgcagt ctggtcaaca
    16261 gcagcaccgt taccaccacc gaactgttta acagtcatag tagaatcttt accattccac
    16321 tgatcaagag tagcgctgtt gccgaaacca cgctgagtca gatcgattga gctgtcatca
    16381 gaaccttggc caacatctgc gccgttaccg ccgccgtgct gggtaatggt cagatcagag
    16441 ttacgggcgt cagcttgcag agcaagagca gagttaccgc caccgtactg gtaaatgttc
    16501 agctctgaat ttgggccgct attattaccg ccaccaccgt ggttaccgcc gccgccgtac
    16561 tgaggaacaa cacctgccag agcgctacca gagaatacga ttgctgcaat tgctgctact
    16621 tttaaaagtt tcatgtaaaa cccccatcgg attgatttaa aagtcgaatg gaaattaacg
    16681 ttgtgtcacg cgaatagcca tttgcgactg tctctgcact acaattgccg ttttttgagt
    16741 accatactgt gtaatatttg ctttattacc agaacctttc tggataatca tcgcagtatt
    16801 accataagca ccttgcgaaa tactggcatc gttggcactg cccgcctgat caatatatgc
    16861 aaggttataa tctcctgtct ggtcaatctt tgcccggttg ctactacctt cttgcgcaac
    16921 aaccgccaaa agttttgagc ctccctgccg taactgagca ctattattag tcccagcttg
    16981 accaattatg gctgcctgat taaatgaaga cttactcaat tcatttaccg cgaagttata
    17041 ttctgaatta gctaaatcat aacctgctgc ggctgcaatc ccaggcgcac ccagtattgt
    17101 taacatcata aataa
//
LOCUS       DAZGAP010000119        17090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGAP010000119 DAZGAP010000000
VERSION     DAZGAP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.120"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHLAM_003369"
     CDS             <1..285
                     /locus_tag="ACHLAM_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO7854844.1"
                     /translation="QPVFPVINDKKSDTGVNVPAGGIEQELKIKPEEEMEQQLPPGIS
                     ESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGDDVEPGDDF"
     gene            285..5555
                     /gene="traI"
                     /locus_tag="ACHLAM_003370"
     CDS             285..5555
                     /gene="traI"
                     /locus_tag="ACHLAM_003370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO7854845.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANLTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQILITDSGQRTGTGSALMAMKDAGVNIYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQTIRSELKTQGVL
                     GQREVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPENMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLESGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGHDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKKLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLSTANIRDGELRRISTWENNPDALALVDSVYHRIAGISRDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVLEPGADREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GFRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDDKTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5575..6321
                     /gene="traX"
                     /locus_tag="ACHLAM_003371"
     CDS             5575..6321
                     /gene="traX"
                     /locus_tag="ACHLAM_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO7854846.1"
                     /translation="MTTDNTNTTRNDSLAARTDTRLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAYIRQSAINRLWGWAMIAQF
                     AYYLAGFPWYEGNILFAFAVVAQVLTWCETRSLWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALLACLLAVIPALNLASSDVAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWPGDFFPVFYACHLTVLGVLAV"
     gene            6380..7240
                     /locus_tag="ACHLAM_003372"
     CDS             6380..7240
                     /locus_tag="ACHLAM_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7854847.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            7343..7900
                     /locus_tag="ACHLAM_003373"
     CDS             7343..7900
                     /locus_tag="ACHLAM_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO7854848.1"
                     /translation="MTEQKRPVLTLKRKTEGETSTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            8054..8257
                     /locus_tag="ACHLAM_003374"
     CDS             8054..8257
                     /locus_tag="ACHLAM_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854849.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            8999..9355
                     /locus_tag="ACHLAM_003375"
                     /pseudo
     CDS             8999..9355
                     /locus_tag="ACHLAM_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            9497..9880
                     /locus_tag="ACHLAM_003376"
     CDS             9497..9880
                     /locus_tag="ACHLAM_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854850.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
     gene            10160..10309
                     /locus_tag="ACHLAM_003377"
     CDS             10160..10309
                     /locus_tag="ACHLAM_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7854851.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            10593..10853
                     /locus_tag="ACHLAM_003378"
     CDS             10593..10853
                     /locus_tag="ACHLAM_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO7854852.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            11078..11152
                     /gene="tap"
                     /locus_tag="ACHLAM_003379"
     CDS             11078..11152
                     /gene="tap"
                     /locus_tag="ACHLAM_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO7854853.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            11145..12002
                     /gene="repA"
                     /locus_tag="ACHLAM_003380"
     CDS             11145..12002
                     /gene="repA"
                     /locus_tag="ACHLAM_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7854854.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            12703..12896
                     /locus_tag="ACHLAM_003381"
                     /pseudo
     CDS             12703..12896
                     /locus_tag="ACHLAM_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12938..13267
                     /locus_tag="ACHLAM_003382"
     CDS             12938..13267
                     /locus_tag="ACHLAM_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7854855.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            13248..13529
                     /locus_tag="ACHLAM_003383"
     CDS             13248..13529
                     /locus_tag="ACHLAM_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7854856.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            13712..13996
                     /locus_tag="ACHLAM_003384"
     CDS             13712..13996
                     /locus_tag="ACHLAM_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO7854857.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            13996..14271
                     /locus_tag="ACHLAM_003385"
     CDS             13996..14271
                     /locus_tag="ACHLAM_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7854858.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(14537..15033)
                     /locus_tag="ACHLAM_003386"
                     /pseudo
     CDS             complement(14537..15033)
                     /locus_tag="ACHLAM_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15310..16185
                     /locus_tag="ACHLAM_003387"
     CDS             15310..16185
                     /locus_tag="ACHLAM_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFO7854859.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLAAGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            16193..16969
                     /locus_tag="ACHLAM_003388"
     CDS             16193..16969
                     /locus_tag="ACHLAM_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFO7854860.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKRKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVSFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT         4325 a         4088 c         4819 g         3858 t
ORIGIN      
        1 cagccagtgt ttcctgtcat caacgataag aagtcagata caggtgtgaa tgttccggcc
       61 ggggggatcg agcaggagct gaaaattaaa ccggaagaag agatggaaca gcaactgcca
      121 cccgggatca gtgaatccgg tgaagtggtg gatatggccg cttatgaggc atggcaacag
      181 gaaaatcatc cggacattca gcagcagatg cagcgtcgtg aagaggtgaa cattaatgtg
      241 caccgggagc gcggggatga tgttgagccg ggagatgatt tctgatgttg agtttcagcg
      301 ttgttaaatc ggcaggcagt gccggaaact attataccga caaggataat tactatgtgc
      361 tgggcagcat gggagaacgc tgggcaggtc agggggctga acagctgggg ctgcagggca
      421 gcgtcgataa ggatgttttt acccgtcttc tggagggcag gctgccggac ggggcggatt
      481 taagccgcat gcaggatggc agtaataagc atcgtcccgg ctacgacctg acgttctccg
      541 ctcccaaaag tgtctccatg atggccatgc tcggggggga taaacgtctt attgatgcac
      601 ataaccaggc tgtggatttt gctgtccgtc aggtggaggc gctggcttcc acacgggtga
      661 tgacggacgg acagtcagaa acggtgctga ccggtaatct ggtgatggca ctgtttaacc
      721 acgacaccag tcgcgatcag gacccacagt tacacacgca tgtggtagtg gctaatctca
      781 cgcagcataa cggcgaatgg aagacactga gcagtgataa agtggggaaa accggttttt
      841 ccgagaacgt gctcgcgaac cgtattgcgt tcgggaaaat atatcagagt gagttacgtc
      901 agcgcgttga ggcgctgggc tatgaaactg aagtggtggg taagcacggt atgtgggaaa
      961 tgccgggcgt accggtggag gccttttccg gacgctcgca ggctatccgg gcggccgtcg
     1021 gggaagatgc ctcactgaaa tcccgggatg tggcggcgct ggatacgcgt aaatccaaac
     1081 agcatgtcga tccggagatc aaaatggccg agtggatgca gacgctgaag gaaaccgggt
     1141 tcgacatccg ggcgtatcgt gacgcggcgg accaacgggc ggagacccgc acgcagacac
     1201 ccggacccgc ttcacaggac gggccggatg tgcagcaggc ggtgacacag gccattgccg
     1261 gattaagtga acgaaaagtg cagttcacgt atacggacgt gctggccagg acggtcggta
     1321 tactgccgcc ggaaaatggt gtgattgagc gggcacgcgc cggtatcgat gaggccatca
     1381 gccgtgagca gcttatcccc ctcgatcgtg agaaggggct gttcacgtcc gggattcatg
     1441 tgctcgatga gctgtccgtc cgggcactca gtcgtgacat catgaaacag aaccgggtga
     1501 ctgtacatcc ggagaaaagt gttccccgga ctgccggtta cagcgatgcg gtcagtgttc
     1561 tggcacagga ccgtccgtcg ctggctattg tgtccgggca gggcggtgca gccgggcagc
     1621 gtgagcgggt ggctgaactg gtcatgatgg cccgggagca ggggcgggag gtgcagatta
     1681 tcgctgctga ccgtcgctcg cagatgaacc tgaagcagga tgaacggctg tcgggtgaac
     1741 tgataaccgg acgtcgtcag ttgctggaag gcatggcctt cacgccgggc agtaccgtta
     1801 tcgtcgacca gggcgaaaaa ctctccctga aagagacgtt aaccctgctg gacggtgccg
     1861 cacgtcataa cgtacagatc ctgataaccg acagcgggca gcgaaccggt acaggcagtg
     1921 cgctgatggc catgaaggat gccggggtga acatatatcg ctggcagggg ggagagcagc
     1981 gaccggccac catcatcagt gaaccggacc gtaatgtccg ctatgcccgg ctggccggag
     2041 attttgcggc cagcgtgaaa gccggagaag agagcgtggc acaggtcagc ggagtacggg
     2101 aacaggccat tctgactcag accattcgca gtgaactgaa aacgcagggc gtactcgggc
     2161 agagggaggt gaccatgacg gcgctgtcac cggtctggct ggacagccgg agccgttatc
     2221 tgcgggatat gtaccgcccg gggatggtga tggagcagtg gaacccggag acacgcagtc
     2281 atgaccgcta tgttatcgac cgggtgacag cgcagagtca cagcctgacc ctgcgggatg
     2341 cgcagggcga aacgcaggtg gtgcgtattt cttctctgga cagcagctgg tcgctgttcc
     2401 ggccggaaaa catgccggtg gcagacggcg agcgtctgag ggtgacaggg aaaattcccg
     2461 gactccgcgt ctccggcggt gaccgcctgc aggtggcatc cgtcagtgaa gatgcgatga
     2521 cggttgttgt gccggggcgg gctgaaccgg ccaccctgcc tgtggctgat tcaccgttta
     2581 cggcactgaa gctggagagc ggctgggtgg aaacgcccgg gcattccgtc agtgacagtg
     2641 cgacggtttt tgcctccgtc acacagatgg caatggacaa cgccaccctg aacggtctgg
     2701 cccgcagcgg tcatgatgtc cggctgtatt cctcgctgga tgaaacccgt actgcagaaa
     2761 aacttgcccg ccatccctcc tttacggtgg tttctgagca gataaaggcg cgtgccggtg
     2821 agacattgct ggaaagtgcc atcagtcatc agaagtccgc acttcacacg ccggcacagc
     2881 aggccattca tcttgccctt cctgtgctgg aaagtaaaaa actggccttc agcatggtgg
     2941 acctgctgac agaggcaaag tcgtttgctg cagaaggaac cggttttact gaactgggag
     3001 gggaaatcaa tgcgcagata aaacgcggtg atttgctgta tgtggatgtg gcaaaaggct
     3061 atggcacagg cctgctggtt tcccgtgcgt cgtatgaggc agaaaagagc attcttcgcc
     3121 atattctcga aggtaaggag gcggtcacgc cgctgatgga gagggtaccc ggcgaactca
     3181 tggagacact gacgtcgggg cagcgtgccg ccacccgcat gatactggaa acgtccgacc
     3241 gtttcacggt ggtgcagggt tatgccggtg tgggtaagac cacacagttc cgggcggtga
     3301 tgtcagccgt gaacatgctg ccggagagtg agcgtccccg tgtcgttggg ctggggccca
     3361 cgcaccgtgc ggtcggggag atgcgcagcg ccggcgtgga tgcacagaca ctggcgtcct
     3421 ttctgcatga cacgcagctg cagcagcgca gcggagaaac gccggatttc agcaacacgc
     3481 tgttcctgct cgatgagagc tcaatggtgg gcaataccga catggcacgg gcatacgccc
     3541 tgattgcggc cggtggtggt cgtgccgtgg ccagcggtga cacggaccag ctgcaggcca
     3601 tcgcgcccgg tcagcctttc cgtctccagc agacgcgcag tgctgccgat gtggccatca
     3661 tgaaggagat tgtgcgtcag acgccggaac tgcgggaggc ggtatacagc ctgattaacc
     3721 gggatgtgga aagggcactg tccgggcttg agagtgtgaa accgtctcag gtgccacgtc
     3781 aggagggcgc atgggcaccg gagcactccg tgacggagtt cagtcacagc caggaagcga
     3841 aactggcaga agcgcagcag aaggcgatgc tgaaaggtga gacttttccg gatgtcccca
     3901 tgacactgta tgaagccatt gtccgcgact ataccggcag gacaccggaa gcacgggagc
     3961 agacgctgat tgtcacgcac ctgaatgagg accggcgcgt actgaacagc atgattcatg
     4021 atgcacggga aaaggccggt gagctgggga aagagcaggt catggtgcct gtcctgagca
     4081 cagcgaatat acgtgacgga gaactgcgtc gtatctccac ctgggagaat aacccggatg
     4141 cccttgccct ggtggatagt gtgtatcacc ggattgctgg tatcagcagg gatgacgggc
     4201 tgataaccct ggaggatgcg gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg
     4261 ctgaaggcgt cacactgtac accccggaca ccatccgggt ggggaccggt gaccggatgc
     4321 gcttcacgaa gagtgaccgg gagcgcggtt atgtggccaa cagcgtctgg acggtgacag
     4381 cggtttccgg tgacagtgtc acgctgtcgg acggtcagca gacccgggtg attcgccccg
     4441 gccaggagcg ggcagagcaa catattgacc tggcttatgc catcaccgct cacggtgcgc
     4501 agggggcgag tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac cggaaacaga
     4561 tggccggctt tgagtcagcc tacgtggccc tgtcgcgtat gaagcagcat gtgcaggtgt
     4621 acaccgataa ccgtcagggc tggacggatg ccatcagcaa agccgtacag aaaggaacgg
     4681 ctcacgatgt gctggagccg ggggctgacc gggaggtcat gaatgcgcag cggctgttca
     4741 gtacggcgcg ggagctgcgg gacgtggcag ctggtcgtgc cgttctccgt caggcagggc
     4801 tggccggggg agacagtcct gcacggttta ttgcaccggg gcgcaaatat ccgcagccgt
     4861 atgtggccct gccggccttt gaccgtaacg gcaagtccgc cggtatctgg ctgaacccac
     4921 tgaccacgga tgacggaaac ggatttcggg gattcagcgg tgaaggccgg gtgaaaggta
     4981 gcggggatgc gcagttcgtg gccctgcagg gcagccgtaa cggagaaagc ctgctggctg
     5041 ataatatgca ggatggtgtc cggattgccc gtgataatcc tgacagcggt gtggtggtaa
     5101 gaatcgccgg cgaaggtcgt ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg
     5161 gggatatacc ggacaacagc gtccagccgg gagcgggaaa tggcgaaccg gtcacggcag
     5221 aggtactggc acagcggcag gctgaagagg ccatccgccg tgaaacggag cgccgcgcag
     5281 atgaaattgt ccgtaaaatg gcagagaaca aacctgacct gccggatgac aaaacagagc
     5341 aggctgtcag ggaaattgcc gggcaggagc gtgaacgggc tgtcacttct gaacgggaag
     5401 ccgcactgcc ggagagcgtg ctgcgtgaac cacaacggga gcgggaagcg gtccgggagg
     5461 ttgcccggga aaatctgttg caggagcgac tgcagcagat ggagcgggat atggtccgcg
     5521 acctgcagaa agagaaaacc ctgggcggag actgatacag gaagacaaac gctgatgaca
     5581 acggataaca cgaacacgac acgtaacgat tcactggctg cccggaccga tacccggttg
     5641 cagtcgttgc tggtctggtc acccggacag cgggatatca tcaaaacggt ggcactggtg
     5701 ctgatggtac tggaccatgc aaaccgcatt ctgcatctgg accagacgtg gatgttcctg
     5761 gccgggcgcg gggcatttcc actgtttgcc ctggtgtggg ggctgaatct gtcccgccat
     5821 gcgtatatcc ggcagtcggc cattaaccgg ctgtggggat gggcaatgat tgcccagttc
     5881 gcatattatc tggcaggctt tccctggtat gaggggaata tcctgtttgc ctttgcagtg
     5941 gtggcacagg tgctgacgtg gtgtgagacg cgcagcctgt ggcgtactgc cgcggccata
     6001 ctcctgatgg cactgtgggg gcctttgtcc ggcaccagtt acggcattgc cgggctgctg
     6061 atgctggcag tcagtcaccg gctgtaccgg gcggaagaca gagcggaacg tctggcgctg
     6121 cttgcatgcc tgctggccgt tattccggca cttaaccttg cctccagtga tgtggcggcc
     6181 gtagctggtc ttgtaatgac ggtgctgacc gttggtctgg tgtcgtgtac agggaaatca
     6241 ttaccccgtt tctggcccgg agattttttc ccggtattct acgcctgtca tcttactgtg
     6301 ctgggcgttc tggctgtgtg actggtatga tgctggcgct gattctgctt attaataaac
     6361 aacataacac aggatattta tgaaaattac ggatcacaaa ctccctgaag gtattgctct
     6421 gacatttcgt gtcccggaag gaaatataaa acatcctttg attctcttat gccatggatt
     6481 ttgcggtatc cggaatgttc tgctgccttc ctttgcgaat gcgtttacag aagccgggtt
     6541 tgcaacgatt acttttgatt atcgtggatt cggtgagagt gaaggggaac gaggtcgtct
     6601 ggttccggca atgcagaccg aagatattat ttcagttatc aattgggcag aaaagcaggt
     6661 atgtattgat aatcagcgta taggattatg gggaacatcg ctgggtgggt gtcatgtatt
     6721 caatgcagct gcgcaggata aaagagtgaa aagtattgtc agccagctgg cttttgctga
     6781 cggtgaggtt ctggttaccg gagaaatgaa tgaactggag aaagcttctt ttctgtccac
     6841 gctgaataaa atggcagaaa agaagaaaaa taccgggaaa gagatgtttg tgggggttac
     6901 ccgggtactg agtgataatg aatcaaaggt attcttcgag aaggtaaaag gtcagtatcc
     6961 tgaaatggat ataaaaatac ctttccttac ggttatggag acgttgcaat ataaaccggc
     7021 agagagcgca gccaaagtac agtgcccagt tttggttgtt attgccggcc aggatagtgt
     7081 taatccacca gaacagggaa gagccttata tgatgctgtt gcttctggta ctaaggaatt
     7141 atacgaggaa gctgacgcat gccattatga tatttatgaa ggcgcgtttt ttgaacgtgt
     7201 ggcggcagta cagacgcaat ggtttaaaaa acatctttaa ttgtcggtat taatatgtca
     7261 gaggtcgttg aggtcagctt aatatcatag tgagtagttt caacgacctg ggggacatat
     7321 tttcacaaag gtcagggcaa tcatgacaga gcagaaacga ccggtactga cactgaaacg
     7381 gaaaacggaa ggggaaacat cgacccggag ccggaaaacc atcatcaatg tcaccacgcc
     7441 accaaaatgg aaggtgaaaa agcagaaact ggcggagaag gctgcccggg aagcagagct
     7501 ggcggcgaag aaagcacagg ccagacaggc gctgtccatt tatctgacgc tgccctcgct
     7561 ggatgaggcg gtgaacactc tgaaaccctg gtggccggga ttgtttgacg gtaacacgcc
     7621 ccggcttctt gcctgtggta tccgggaggt tttactggat gaggtgtttc agcggaatat
     7681 cccgctctct cataaaaaac tcagccgggc gctgaaagcc atcacccgtt cggaaagtta
     7741 tctgtgtgca atgaaagccg gcgcctgccg ctatgatacg gaaggctatg tgacagaaca
     7801 tatcacacag gaagaagaac agtatgcgca ggcgcgcctg gaaaaggtgc gcagacagaa
     7861 ccggatcaag gatgagctgc gggcgatcct ggcagaataa taaaaacgcc ctctgtatga
     7921 cagagggcaa cagcgaaaaa atcgggaaaa cagacatcca gagttttgat ggagaaaaac
     7981 acccaaccgt tcagggatgt ttcataatgt ttctgacata atccgggagc cgggtcaaga
     8041 ggggggataa aaaatggctg tatccagtgg attatctgag cacagcggta tttatcgcta
     8101 tcagcgcgtg gggcgtcatg ccgtccgtca ggagattcgt ggttttttca gggaagccgc
     8161 tgaggcctgg cagaatgcgg cccggctggc accctgtgaa gcatggcggg attttgccct
     8221 gaagagagca tctgcatgcc ggtgtaagtg ccgataaaaa taacccccgt tgcggagcgc
     8281 cgggggatca gacttatatg gcagatcgtt attattatag tggtgtaaga aggagttgcc
     8341 ctgtctttca aacggacatc acagaattaa catatttcac atcccgggta agccaacagg
     8401 gataaaaaat gtgatgaatt tcaagaagca gaaggtgaca gtggcatgca taacagtacg
     8461 gttatgtccg gtgaatctga cagacagccg ggctgtattc gcggcaggat aaagaaaaac
     8521 cgggagatga ctctcccggt ccgtcttatc gctaatcccc tgagcagggc atgtatgact
     8581 tatctatgac cgttatattc agcgccacat ccggtagcct gcgatataac acagttatcc
     8641 tatcagtaag agcaggtatg atgaatacac ggagattaaa aatataatct ccgtcacatg
     8701 ttacccccgg aaaccggtaa cgccgtcacg ggaaatacgt gacagaaaaa gcagcctccg
     8761 ttactccagg tatcagaagg ctgaataatg catgagactt tctttcttat gtgtctggtc
     8821 aggcggggtg accagcgttt ttagtaacac atagccatat gtgtattaag ccacctcatc
     8881 ttattttgtc gaattgatca caattcaacg gtttattttt ggcgtgatgc gtgagccgtt
     8941 attcatcccg gcatggaatt catgcaatca cgaaaagtaa tcagtgacgg agagtaaaat
     9001 gaagaaatat ataccagtgg tattatttgt tttttcatgt caggtatttt ctgctgatat
     9061 tcatggacgt gttgtgcgtg ttctggatgg cgataccatt gacgtcatgt tatctcagca
     9121 tccggtgcgt gtccgtcttg ttaatattga tgcacctgaa aagaaacagg actatggccg
     9181 gtggtctgaa aaaataatga aatctctggt tgcgggaaaa acggttactg tcacatattt
     9241 tcagcgagat cgttatggtc gtattctcgg gcaggtttat gcaccagacg gtatgaatgt
     9301 taatcagttt atggtccgtg caggcgctgc atgggtttat gagcagtaca acaccgatcc
     9361 tgttccgccc tgggtatggc gctcacggaa ataatgatat ttctgtggct attgagttct
     9421 cattgaaatg cgatatttaa aagcatgaag catattacat tattaactta tttctctgtt
     9481 aatgaaaggt gaacaaatga actatcaggc agtatcagaa ttaatcacat cgtctaatca
     9541 taatgttctt attgtggggg actcagcttc tgaagttgat agctttttaa ataaactgaa
     9601 tattactgat tataagtatt atgatttcag ccagatttac agttgttctg acagaacatt
     9661 aaatgattat gcggttattt ttatcaggga tgcactgaac gccagtgagc atattattat
     9721 ttttaattgt acaggatggc ctgatttgaa taatgaatct gccgtcatgc agtttgccag
     9781 agtggcacga aaatcaggaa aacaactgat agtcgcagta agagaacagg atatgaaaaa
     9841 aatggaagca gagtttggaa ggatcataaa aatccactga cgccttcgcg ttgctcagtt
     9901 gtccaacccc ggggacggga aaaagcaagt tttccccgcc cccggcgttt ctgtagtgaa
     9961 aagcgggaaa tggagggaat ggtcagtaac agggaatgtg gtattatcgc ggcgggtgtc
    10021 tgagcctttc tggttcaggc aagacgcagg taccagaaat gcgaagaccc cacttgttaa
    10081 tccattaact cgtgaggcct gcatgaagta cttgaacact actgattgta gcctcttcct
    10141 tgcagagagg tcaaagttta tgacgaaata tgcccttatc gggttgctcg ccgtgtgcgc
    10201 cacggtgttg tgtttttcac tgatattcag ggaacggtta tgtgagctga atattcacag
    10261 gggaaataca gtggtgcagg taactctggc ctacgaagca cggaagtaag ctgccgggcg
    10321 gggacggaag tccccgcttt ccggaagtgt gaggtatttc aggggcagac acccgacatg
    10381 ccagaaaccg ccggtcccgc ccggggccgg cacacaggtt aaggcatttc ctgtttttca
    10441 gccatttcgc aacaaaaatc acattaaaca acagtaatca ccgttgattt gtgcgccaac
    10501 acagatcttc gtcacaattc tcaagtcgct gatttcaaaa aactgtagta tcctctgcga
    10561 aacgatccct gtttgagaat tgaggaggcg agatgtcgca gacagaaaat gcagtgactt
    10621 cctcattgag tcaaaagcgg tttgtgcgca gaggtaagcc tatgactgac tctgagaaac
    10681 aaatggccgc tgttgcaaga aaacgtctta cacacaaaga gataaaagtt tttgtcaaaa
    10741 atcctctgaa agatctcatg gttgagtact gcgagagaga ggggataaca caggctcagt
    10801 tcgttgagaa aatcatcaaa gatgaactgc agagactgga catactaaag taaagacttt
    10861 actttgtggc gtagcatgct agattactga tcgtttaagg aattttatgg cgggccacgc
    10921 cgtaaggtgg cagggaactg gttctgatgt ggatgtacag gagccagaaa agcaaaaacc
    10981 ccgataatct tctttaactt tggcgagtca gaaagattac cggggccatc taaaaaccgt
    11041 atagccaaca attcagctat gcggggagta tagttatatg cccggaaaag ttcaagactt
    11101 ctttctgtgc tcgctccttc tgcgcattgt aagtgcagga tggtgtgact gatcttcaac
    11161 aaacgtatta ccgccaggta aagaacccga atccggtgtt tacaccccgt aaaggtgccg
    11221 gaacgctgaa gttctgcgaa aaactgatgg aaaaggcggt gggcttcacc tcccgttttg
    11281 atttcgccat tcatgtggcg catgcccgtt cgcgtggtct gcgtcgacgc atgccaccgg
    11341 tgctgcgtcg acgggctatt gatgcgctgc tgcaggggct gtgtttccat tatgacccgc
    11401 tggccaaccg cgtccagtgt tccattacca cactggccat tgagtgcgga ctggcgacag
    11461 agtccggtgc aggaaaactc tccatcaccc gggccacccg ggccctgacg ttcctgtcag
    11521 agctgggact gattacctac cagacggaat atgacccgct tatcgggtgc tacattccga
    11581 ccgatatcac gttcacatcg gcgctgtttg ccgcccttga tgtgtctgag gatgcagtgg
    11641 cagctgcgcg ccgcagccgt gtggtatggg aaaacagaca gcgcaaaaag caggggctgg
    11701 ataccctggg tatggatgag ctgatagcga aagcctggcg ttttgtccgt gagcgtttcc
    11761 gcagttacca gacagagctt aagtcccacg gcatgaaacg tgcccgtgcg cgccgcgatg
    11821 caggcagaac ccgccaggac attgtcaccc tggtgaaacg gcagctgacg cgtgaaatcg
    11881 cggaagggcg tttccgggga agcctggagg ccgtgaagcg cgaaatcgac cgtcgtgtga
    11941 aggaacgcat gataatgtcg cgtaacaaca actacacacg gctggccact gcctccccct
    12001 gagttttacc tcatctgaat aatccggcct gcgccggagg catctgcacg cctgaagcct
    12061 gtcagcgaac aaaaaaacag caccacatac aaaaaacaat ctcatcatcc cgcttctggt
    12121 gcatccggcc cccctgtttt cgatacaaaa catgcctcac tgacggggaa ttttgcttat
    12181 ccacatttaa ctgcaaggga ctttcccata aagttacaac cgttcatgtc ataaagcgcc
    12241 agccgccagc cttacagggt gcaatgtatc ttttaaacac ctgtttatat ctcctttaaa
    12301 ccacttaact acattcattt aaaaagaaaa cctattcact gcctgtcctg tggacagaca
    12361 gatatgcacc tcccaccgca agcggcgggc cccgaccgga gccactttag ttacaacact
    12421 cagatacaac caccagaaaa acccaagtcc cgcgcagaac tgaaaccaca aagcccctcc
    12481 tcataactga aaagcggccc cgccccggcc caaagggccg gaacagagtc gcttttaatt
    12541 atgaatgctg taactaatca tcttcatcgc tgtcagtctt ctggctggaa gtcctcagta
    12601 cacgctcgta agcggccctg acggcccgct aacgcggaga tacgccccga cttcgggtaa
    12661 accctcgtcg ggaccactcc gaccgcgcac ataagctcta tcatagctga cagcgggtat
    12721 ggctttgcag ggtggggatg ggtaaggtga aatctatcaa tcagtaccgg cttacgccgg
    12781 gcttcggcgg ttttactccg gtatcacatg taaccacagc gtgccgcctt acatgcgctg
    12841 acgcgcataa acaacaagtt gctacattag cttacaggtt aattcacctt gcataagatt
    12901 agcatatagg ttaatgttta tcgaaatggg aggtcatatg tttagcgttg tttatcaccc
    12961 ggaagccagg gaagaagcca cagcattacc cataaaaatc agggtgaagt ttgaccgtct
    13021 catcagcaaa ctggaatatg acgcccgatt actgcgggaa ccggatacaa agcccctggg
    13081 ggatggactt tttgagatcc gcacgatggg gacggacata gccagaggta tctgggtata
    13141 ccacaaaggc aataccatca tcatgctccg ggttttcatc aagaagtctc agaaaacgcc
    13201 agcaaaagaa atcgatctcg ccaaaaagcg gttagcggag gtacttaatg aaactggtaa
    13261 gccataaaga actgcataac gagtggatgc aggacgacga atatcgcgct gcatggcagg
    13321 aagaagaacg taaggagaaa ctcagggagt tgcttgcaga atggcgtaag catgacaatt
    13381 tgacaaaagc gcaggttgct gaacgcatgg gggttactcc tcctgttatt tcccgcctgg
    13441 aaaacaacat cactaaagcc agcatcgaca ccctgactcg ctatgcccat gcgtgcggta
    13501 tcaaaaaacc tgttattacg ctgtactgaa ctctgctcta tggctgccag ttctggagaa
    13561 atcagtaact aaattttaat tagctacgtt atggtaaatc tgcctgatgt gtcgatttct
    13621 ctgaaagtaa ttcggcttct gtgggctgcg tttgccttgt gccacctgaa tgtaatcact
    13681 atgattaaat atgattaaca actaatcgga tatacaaatg aaaaacaata ccgcacaggc
    13741 aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg accagatggc
    13801 cgccagactg gaacgctccc ggggctgggt tatcaaacag gcactttctg catggcttgc
    13861 ccaggaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga tatccggaca
    13921 gattatcgac catcaggctg tacaggcctg ggcagacagc ctcagtactg acaatccgtt
    13981 accggttcca cgctgatgga actgaagtgg accagtaagg cgctttctga tttggcgcgg
    14041 ttatatgatt ttctggtgct ggccagtaaa cctgccgccg ccagaatggt acagtccctg
    14101 atacaggcac cggtcattct gttaactcat ccacgtatgg gagaacagtt gtttcagttt
    14161 gaacccaggg aggtcagacg gatttttgct ggcgagtacg aaatccgtta cgaacttaat
    14221 ggccagacta tttatgtatt gcgcctgtgg cacacacgag aaaacaggta gattgcgtgt
    14281 ggggacaact taacatagcc tgcagatatt aattcaggaa cattattatc aggccagaga
    14341 ttttgcattc cattaaataa tcttgacgta taggaaattg aaaaactgag cgatacccct
    14401 gtccgcgcct tatgccacgt gagcttttct gcttttccca ccactccggt gttttctctc
    14461 tatgtatggt cggtttttat accttaatgg gccgtttttt atacttttcc cggattgcag
    14521 gcgcacctct cctgcatcag ccagggatat acctcagcag gcttatatcc cgcgcgtttt
    14581 tcttcagccc tgatacattc agccccgcaa tggcccggcg gtataccatc cggcagccac
    14641 acagcacgta ttcgaacggg tcacgactca ggaactgcgc ttcaccattt gtgcatagca
    14701 cactttcgcc actgcttccg gtttatccat ccccagagca cggtacacct gcggcagctt
    14761 ctctccacac acccggttgg caaggaaccc gaagtacctc accatcttaa aaaacttctc
    14821 cgggatgtgc tgtttcagcc tcgcgaccag ctcacgctgt gtcagcgttt ccgtcgccgt
    14881 ttctcccgtt ttgtggtcca ggtaacggaa gctcaggctc gcccctccgt tgtaatgtgc
    14941 cagccgggag gctgctatcg gcggcttctt cagataacga cccaggtggc gcgccgtatt
    15001 cctcccgtcg gccgttttct tcgacatgta catcaggcgc ggaactcatc ggggcgcggg
    15061 aatgaaatcc cattcagact ccggatagat tcaagcaagc ccacttatcg tcaaaatcgg
    15121 tgttgcaaaa acgggagtga ccatagatcc gttttccaag cggaccccat aatatcggct
    15181 ggtaaacatc actcgctagt gcattctttt tgttggtaat gataattatt tacatttatt
    15241 ctttgatgtt ttatcataat taatgttttg tcaataaata tatggaaaca tattgtttca
    15301 gatgggaata tgagaaagtt catccttatt tctatgataa cgttagctag tgttagtctg
    15361 ggagcctgca gacagaatgt caccataaag caggatgcgc cagggcagaa agctttcctg
    15421 actgacgggc agatttacga tttacacagt ggcaaaatta tttctagcag cgagcttttg
    15481 gcggatcttg ctaccgctcg acaccttatt attggtgaaa aacacgataa tgccgagcat
    15541 catcagattg agctttggct tattcaaaat ctgctcatcc agcgaccaca ggggagtgtg
    15601 ttgcttgaga tgttgacgtc agagcaacag ccccgagtta accaagttaa atgttggctg
    15661 aaagataatc ctgtggttcg agatagccgt gttcaggaac tacttaactg gcaaaaaggg
    15721 tggtcctggg agatgtatgg cgacatcgtc atgcagctat tacgtgggcc ttacccgctg
    15781 ctcaatgcca acattggccg ggagcagatg ttggcactgt acaaaaaaaa cgaatttcct
    15841 aaaggaaaaa aatccactgc acctgtggta caagaagctc tgcgtgaaac catcatagct
    15901 atgcatgaag gcaaccttga aagccagcaa ctgacgtcaa tgctctctgt tcagcagcag
    15961 cgggatcgct acatggcccg ccagcttctg agtgccccgg taccgtcgtt gttgattgca
    16021 ggtggttatc acgcgtcaaa aagcatgggc gtaccgttac acatggaaga tcttgccgcc
    16081 ggcacccatc cagtggtgtt gatgcttgca gaaaaaggaa tgaacatcac agtagatcat
    16141 gctgactacg tgtggtttgt agcgccagat acaacaaaaa ggtaaaccat ggatgatgaa
    16201 tattctccac ttcccacagt cggtaaaatg gagcagctgg tttatctgtt ctctactgct
    16261 tcatggactg attttccttg cgtttatctg gcgtttttcc gaagttcagc ccgctatgtc
    16321 tcctgcaccg gcgatcatgc tgcaatgggc agaaattata gaggctcctt cttctccact
    16381 ttcgcttccg gtgggaatcg ctcaacagga gtccgcagtg acagaagaaa agcaacaaac
    16441 tgaggacaga caacaacgtc ctgtcactga agacagtgat gcaaccattg aaataaccag
    16501 gaagaaaaag tcatccgatg gagaaaagag aaagacacgt ccacctcgta agataaaggc
    16561 tcaaaccagc gacagcaacc ccacagccgt ttccagtaat gctgcacctc aggctctggt
    16621 cgaatcgtcc cgtattgctg cgccttttaa cagtgactca acaaagcgag ataattctga
    16681 ggcatcttgg gaaagcagag taaaaggcca ccttaaccgt tataaacgtt accctggcga
    16741 tgccagaaaa cgtgcacgaa ccggcacagc cgtcgtgagt tttaccgtaa acacggaagg
    16801 cactattgtc agtagtttct tggaaatctc ttcaggaacc ctttctcttg accgcgaagc
    16861 tattgctgtt ttagaacgcg ctcaaccact tcccaaacca ccgcctgaaa tattggaggg
    16921 gggattattt aaagtaaaaa tgccgattac ctttaagttg aaggagtaaa gggataattg
    16981 gccgttcaag ttcatttaac tgaaatttaa ttttcatttt tatgctgaac attttatcaa
    17041 acttttccac tgtcgaaaaa atcaaaaaga cagtagatca taattaataa
//
LOCUS       DAZGAP010000120        16833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGAP010000120 DAZGAP010000000
VERSION     DAZGAP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.121"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHLAM_003389"
     CDS             complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHLAM_003389"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7854861.1"
                     /translation="MLEDLKRQVLEANLALPKHNLITLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHLAM_003390"
     CDS             complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHLAM_003390"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO7854862.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGIKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHLAM_003391"
     CDS             complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHLAM_003391"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO7854863.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4471..5349
                     /gene="araC"
                     /locus_tag="ACHLAM_003392"
     CDS             4471..5349
                     /gene="araC"
                     /locus_tag="ACHLAM_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO7854864.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            5435..6199
                     /gene="yabI"
                     /locus_tag="ACHLAM_003393"
     CDS             5435..6199
                     /gene="yabI"
                     /locus_tag="ACHLAM_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7854865.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLAPLISAVGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHLAM_003394"
     CDS             complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHLAM_003394"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO7854866.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHLAM_003395"
     CDS             complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHLAM_003395"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO7854867.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHLAM_003396"
     CDS             complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHLAM_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO7854868.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9595..9694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHLAM_003397"
     CDS             complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHLAM_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO7854869.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNSLGWFDFKSAWFAPPDP"
     gene            11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHLAM_003398"
     CDS             11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHLAM_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO7854870.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHLAM_003399"
     CDS             complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHLAM_003399"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO7854871.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHLAM_003400"
     CDS             complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHLAM_003400"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO7854872.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHLAM_003401"
     CDS             complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHLAM_003401"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO7854873.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHLAM_003402"
     CDS             complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHLAM_003402"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO7854874.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHLAM_003403"
     CDS             complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHLAM_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO7854875.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT         3712 a         4585 c         4583 g         3953 t
ORIGIN      
        1 ttggctgtgg ttttatacag tcattactgc ccgtaatatg ccttcgcacc atgcttacgc
       61 agatagtgtt tatccagcag cgtttgctgc atatccggta actgcggcgc taactgacgg
      121 cagaatatcc ccatataagc gacctcttcc agcacgatgg cgttatgcac cgcatcttcg
      181 gcatttttgc cccatgcaaa tgggccgtga gaatggacca ggacgccggg catttgtgct
      241 gcgtcgattc cctgtttttc gaaggtttct acgatgacgt taccggtttc ccactcatat
      301 tcaccgttga tttctgcgtc ggtcattttg cgggtgcagg gaatggtgcc gtagaaatag
      361 tcggcgtggg tggtaccggt tgccggaatt gactggcccg cctgcgccca gatggtggcg
      421 tggcgcgagt gtgtgtgcac aatgccgcca atggacggga atgcctgata gagcagccgg
      481 tgagttggcg tgtcggagga gggctttttc gttccttcaa ccacttcacc ggtttcgatg
      541 ctgaccacga ccatatcgtc agcggtcatg acgttgtaat cgacgccgga aggtttgatc
      601 accaagacgc cgcgctcgcg atcaacggcg ctgacattgc cccatgtgag cgtgatcagg
      661 ttatgttttg gcagcgccaa attggcttct aatacctggc gtttgagatc ttctaacatg
      721 ttgactcctt cgtgccggat gcgctttgct tatccggcct acaaaatcgc agcgtgtagg
      781 cctgataaga cgcgtcagcg tcgcatcagg cggtgaatgc cggatgcgct ttgcttatcc
      841 ggcctacaaa atcacagcgt gtaggcctga taagacgcgt cagcgtcgca tcaggcgtta
      901 cacacctgat gcggctactt agcgacgaaa cccgtaatac acttcgttcc agcgcagcgc
      961 gtctttaaac gctggcaggc gggtatcgtt atcaatcacc gtgatttcaa tgtcgtgcat
     1021 ctcggcgaac tggcgcatat cgttgaggtt cagcgcatgg ctgaaaacgg tatggtgcgc
     1081 gccaccagca aggatccacg cttcggaagc agttggcaga tccggttgcg ctttccacag
     1141 cgcattcgcc accggcagtt tcggcaggga gtgcggtgtt ttcaccgtgt cgatgcagtt
     1201 aaccagcaga cggtaacgat cgccgagatc aatcaggctg gcgacaatcg ctggaccggt
     1261 ttgggtattg aagatcaggc gggcaggatc gtctttaccg ccaataccga ggtgctgaac
     1321 gtcgaggatc ggtttctctt ccacagcgat cgacggacag acttccagca tgtgggagcc
     1381 gagcaccagg tcattgcctt tctcgaagtg ataggtgtag tcctccataa aggaggtgcc
     1441 gccctgcaga ccggttgaca tcaccttcat gatgcgaagc agggcggcag ttttccagtc
     1501 gccttcgccc gcaaagccgt aaccctgctg catcagacgc tgtacggcca gaccaggaag
     1561 ctgtttcaga ccgtgcaaat cttcaaaggt ggtggtgaac gcgtggaagc caccttgttc
     1621 caggaaacgc ttaatcccca gctcaatacg cgccgcttcc agcacgttct ggcgtttttc
     1681 gccgtggatt tgtgtggcag gcgtcatggt gtagcagctt tcgtactcat cgaccagcgc
     1741 gttaacatcg ccatcgctga tggagttcac tacctgcacc agatcgccaa ccgcccaggt
     1801 attgacggag aaaccgaact tgatctgtgc ggcaacttta tcaccatcgg tgaccgccac
     1861 ttcacgcatg ttatcgccaa aacggcagac tttcagatga cgggtatcct gtttagagac
     1921 cgcctgacgc atccaggagc cgatacgctc atgggcttgt ttatcctgcc agtgaccggt
     1981 aacgacggca tgttgctgac gcatacgcgc gccaatgaag ccgaactcgc gaccgccatg
     2041 tgcagtctgg ttcaggttca taaagtccat atcgatgcta tcccacggca gcgccgcgtt
     2101 aaactgggtg tggaattgca gcaacggttt gttgagcata gtcaggccgt tgatccacat
     2161 tttggccggg gagaaagtgt gcagccacac taccaggcca gcgcaacgat cgtcgtaatt
     2221 cgcgtcacgg caaatagcgg tgatttcatc cggcgtggtg cccagcggtt tcagcaccag
     2281 tttgcagggc agtttcgctt ccgtattcag cgcattaacg acgtgctcgg catgttgggt
     2341 gacctgacgc agggtttccg ggccatacag atgctggctg ccaattacaa accacacttc
     2401 ataattatca aaaatcgtca ttgtcgtgtc cttatagagt cggaacggcc tgggcagcct
     2461 gtgccggggc ggaagttgga agatagtgtt gttcggcgct catcgcccat tgctgatagc
     2521 ggcgataaag ctgttcaaag cgttgtgcct gttcgctgcg cggttgcagg gttttctcta
     2581 ccgcactggc cattttttgc tgggctgagg ggatgtctgc gtgcactttc gcggcgacgg
     2641 cagcgaaaat cgccgcaccg agcgcacagc actggtcaga ggcaacaatt tgcagcgggc
     2701 gattcagcac gtcgcagcag gcctgcataa tgacctggtt tttccgcgcg atgccgccca
     2761 gcgccatcac gttattgacg gcgatcccct gatcggtaaa gcactccatg attgcgcgtg
     2821 cgccaaaggc ggtggcagca atcaatccgc cgaacagcag cggagcgtcg gtagcgaggt
     2881 tcagatcggt aatcacccct ttcaggcgtt ggttagcgtt tggcgtgcgg cggccgttaa
     2941 accagtcgag caccaccggc aggtgatcca gagacggatt tttggcccat gcttcggtca
     3001 gcgccggaag cagttgtttc tggctggcgt tgatttgctc tttcagttcc ggatgctggg
     3061 cggcaagctg ttccagcggc cagccgagta cgcgaccgaa ccaggcgtag atatcaccaa
     3121 acgccgattg gcctgcttcc agaccgataa atccaggcac cacgctgcca tcaacctgac
     3181 cgcaaatacc tttcactgcc cgctcgccaa cgctctgttt gttggcaatc agaatgtcgc
     3241 aggtggaagt accgataact tttaccagag cgttaggctg tgcgcctgcg ccaactgcgc
     3301 ccatatggca gtcaaacgcg ccgccggaaa tcaccacgct ttcaggtagg ccgagacgct
     3361 gcgcccattc cgggcataag gtgcccaccg gaatatcggc agtccaggtg tcagtgaata
     3421 gcggggaagg caaatggcga ttgaggatcg ggtccagctc atcaaagaaa ctggctggcg
     3481 gcaggccgcc ccagctttca tgccatagcg acttatgccc ggcgctgcaa cgtccgcgac
     3541 gaatatcctg cgggcgggtg gtaccggaaa gcagagctgg cacccagtcg cacagctcaa
     3601 tccacgatgc ggcagattgc gccacggcgc tgtcctggcg agtcacatgc agtatttttg
     3661 cccagaacca ttcgctggaa taaataccgc caatatagcg ggagtagtca acgttgcccg
     3721 gcgtgtgaca taagcgggtt atctcttccg cttcttcaac cgcggtgtgg tctttccaca
     3781 atacgaacat cgcgttcggg ttttcggcaa actccgggcg cagcgccagg acattaccgt
     3841 ccgcgtcaat cggtgcgggc gtcgagccgg ttgtgtcaac gccaatcccg accacagctg
     3901 cgcgctgctc ggcgctaagc tctgcaagca cggttttcag cgccgcttcc attgactcaa
     3961 tgtagtcacg cggatgatga cggaactggt tatttggggc atcacaaaat tgccctttct
     4021 gccaacgggg ataccactct acgctggtgg cgatctcttc accggtggcg cagtccaccg
     4081 ccaaagctcg cacagaatca ctgccaaaat cgaggccaat tgcaatcgcc atcgtttcac
     4141 tccatccaaa aaaacgggta tggagaaaca gtagagagtt gcgaaaaaaa gcgtcaggta
     4201 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa
     4261 taatcaatgt ggacttttct gccgtgatta tagacacttt tgttacgcgt ttttgtcatg
     4321 gctttggtcc cgctttgtta cagaatgctt ttaataagcg gggttaccgg ttggattagc
     4381 gagaagagcc agtaaaagac gcagtgacgg caatgtctga tgcaatatgg acaattggtt
     4441 tcttctctga atggcgggag tatgaaaagt atggctgaag cgcaaaatga tcccctgctg
     4501 ccgggatact cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaacggt
     4561 tatctcgatt tttttatcga ccgaccactg ggaatgaagg gttatattct caatctcacc
     4621 atacgcggcc agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat
     4681 attttgctgt tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa
     4741 tggtatcacc agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg
     4801 ccgtcaatat ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc
     4861 agcgacctgt ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg
     4921 ctggcgataa atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg
     4981 ctccatccac cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg
     5041 gcagacagca attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt
     5101 ctgtcgcatc tgttccgcca gcagttagga attagcgtct taagctggcg cgaggaccaa
     5161 cgcattagtc aggcgaagct gcttttgagc accacccgga tgcctatcgc caccgtcggt
     5221 cgcaatgttg gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg
     5281 gccagcccga gtgaattccg tgccggttgt gaagaaaaag tgaatgatgt agccgccaag
     5341 ttgtcataat tggtaacgaa tcagataatt gacggcttga cggagtagca tagggtttgc
     5401 agaatccctg cttcgtccat ttgacaggca cattatgcaa gcattgctgg aacactttat
     5461 tacccaatcc accgtgtatt cattgatggc ggtggtgttg gtagcctttc tggagtcgct
     5521 ggcgctggtc ggtttgattc tacccggtac ggtgctaatg gcgggactgg gggcgctgat
     5581 tggcagtggc gagttaagtt tctggcacgc ctggctggca gggatcgtcg gttgtctgct
     5641 gggcgactgg atctccttct ggctgggctg gcgttttaaa aagccgttgc atcgctggtc
     5701 atttctgaag aagaacaaag cattactcga taaaaccgaa catgcgctgc atcagcacag
     5761 catgttcacc attctggtcg gtcgctttgt tggcccgacg cgtccgctgg tgccaatggt
     5821 ggcgggaatg ctggatctgc cggtggcaaa attcattacg ccgaatatta tcggctgcct
     5881 gctgtggcca ccgttttact tcctgccagg gattctggcg ggtgcggcga tcgatattcc
     5941 agccggaatg cagagcggcg agtttaaatg gttgctgctg gcgacggcgg tatttttgtg
     6001 ggttggtggc tggctgtgct ggcggttatg gcgcagcggt aaagcgactg accgtttgag
     6061 tcattatttg tcgcgcggtc gtttgttgtg gctggcaccg ttaatttctg ccgtcggtgt
     6121 agtggcgctg gtggtgttaa ttcgccaccc gttgatgccg gtgtatatcg atattttgcg
     6181 taaagtggtt ggggtttagg ggatagcctt gtgcgggttg cctgatgcga cgcttgccgc
     6241 gtcttatcag gcctacaaaa cgcactgtcc gtaggtcgga taaggcgttc acgccgcatc
     6301 cgacagtgca tactaacccg taatccccaa tattgccgaa gcactcgcct taccgctcaa
     6361 caactcatcg gtcataccct gccaggcgat gcgcccgtcg gcgaccacca ccgagcgcgt
     6421 ggcgatccgc gccgcatctt ccacactgtg cgacaccatc aatagcgtca ttttttgctg
     6481 ttggcagctc gtgctcacca gcgtcaacat ctcctgacgt aacgccggat cgagcgcaga
     6541 gaacggttca tcgagcaata aaatcggctg ttcgcgcacc agacaacgcg ctaacgccac
     6601 gcgctgtcgc tgaccgccag aaagctcgcc cggtaagcgc gccattaaat tatcaatccc
     6661 catctgacgg gcaatcgcgt gcattttctc ctgttgtgcc gcgttcagct tcaatcctgg
     6721 attaagcccc agcccgatgt tctgcgcgac cgtcaggtga ctgaacaggt tgttttcctg
     6781 aaacagcatc gacaccggac ggcgtgacgg cggcgtcgtg gtgtgatcaa cgccgtcgat
     6841 agtcagcgaa ccgctggctg gcgtcagaaa accggcgatc aaattcagca gggtactttt
     6901 acccgcgccg cttggcccga ggatcgccac ctgctcgctg cgttccaccg ttaagctaaa
     6961 acgcatcggc aaatggtggt aaagccaggt gatatcagtc agttttaaca tttcgccccg
     7021 gtagtttttc aatcacggta aacagcagaa aacagagtag cagcagaatt aacgcggtga
     7081 ccgcgccgtc ctggctgcga taggagccaa tttgctggta gagataaaac ggcagggtgc
     7141 ggaaatcatc gttaccaaac aacgccacca cgccaaaatc accaatcgat agcacacaag
     7201 caaaggccag tgcctgcgcc agtgggcgtt taagggcgcg cagctccacc acttttaagc
     7261 gtgaccagcc ttcaatgccc agcgactgac acaacatact gtaacgcgcg gtgatatcgc
     7321 gcatcgggtt ttccagcact ttcagcgcat aagggatcgc cattaacgca ttggtgaaaa
     7381 tcacaatgcc gtcagcagat tgtggcaggc cgatagtgtt gttgagcagt aaaaagaagc
     7441 cggtagccag cacaatcccc ggcatcgcga ggatcaacat gccgctcatc tccagcgcct
     7501 gacccgccag cattttctgc cgcgcccgca gttcgcgact gctccatagc agcatcatgg
     7561 tcagcactac gcataatacg cctgccgcca gcgcaatacg caacgaggtc cacagcgcct
     7621 gccacagcac cggctgcgcc agcacttccg gcaactggcg atttatccca tcgacgagca
     7681 ccgccagtaa cggtggcagc aacagcaaca gcgccagcac aattaacacc gtgtcgcaaa
     7741 tgcggctatg cagacgatcg tccgggtcgc gccagccttg cagcagcgta gtgccgggcg
     7801 caatggcctt actcaatcgc tgactcaaca gcaccagccc aaggcaacac accatctgga
     7861 tcagcgccag catcgccgcg cgggcaggat cgtagtcgta actcagcgcc tgataaatag
     7921 ccagctcgat agtcgtcgct tgcggaccgc cgcccagcga caacacggtg gcgaagctgg
     7981 cgaaacagag cataaagata agcgcggcaa ccggcgggat ttgtcgccgt aaccacggcc
     8041 attcgacgaa gcggaaaaaa tgccagccgc gcatcccaag ctgggcggca agctgacgtt
     8101 gttccccggg aatgttttcc agtgcctgga gtaataagcg gctcgccatc ggcagattaa
     8161 aaaatacatg cgccagcaaa atgccttgca ggccgtaggg cgaaaacgtc cactccagac
     8221 caagcgattg ccagagcgat gccagccagc cctggcgacc atagacgcta agaatgccga
     8281 aaacagcaac cagcaccggg aggatcaagg tcatggcaca cagacgcaac agcgccagcc
     8341 gtcctggaaa gcgcctgcgg tagagggcgc gggcaaggaa tatcgcgggc acgacagaga
     8401 gcagcgccga gagaaacgcc tgccagaagg agaagcgcac cacatgccac aggtagctgt
     8461 cctgccagat tgccacccag tcaccctgcg gcgcgttcca ccacagggcg agaaacgcag
     8521 ccagtgcaac cgccaccacc agcgtggcgg cgcttattcc tggaattaac cagccgggaa
     8581 ttaacggctg acggcgcgtt gccattcgct aatccatgcc tgacgttgtg ccgccacttc
     8641 ggctggcgtg aactccagcg tggttgccgg tttggtcaat tgttcaaaac cggcaggcag
     8701 cgtgacgttt gccaccggat acatccagtt gccggttggg atcgcattct gaaaagccgg
     8761 agaaaccata aactggagaa atttttgcgc cagctccggc tgcttgctgg cagcggtgcg
     8821 ggcggcgact tccacctgca gatagtgacc ttcgctgaag tttgcggcgg cgtagttatc
     8881 tttcttctct tcgagaatgt gataagccgg agaggtggtg taactcagta ccagatcgct
     8941 ttcacctttt aaaaacaggc cgtaggcttc gctccagcct ttggtgaccg tgaccgtttt
     9001 cttcgccagt ttctgccagg cttgtggggc gtcatcgcca tagacttttt gcatccacaa
     9061 cagcaggccc agccccggcg tactggtgcg cggatcctga taaatcaccc gccagttttg
     9121 atcgctctca acaagctctt tcaggctttg cggcggattt ttcagtttgt tcttgtcata
     9181 gacgaaggcg aaatagccgt aatcaaacgg tacgaaagtg tcatttttcc agccgccggg
     9241 aacgttaacg gcatccgctg ccacgccgct tttggcaaat agtccggttt tactggcggc
     9301 gtctaacagg ttgttatcca gccccagcac cacatcggct ttactgtttt tgccttccat
     9361 ccgcagacgg ttgagcagcg aaacgccatc ttccagcgcc accagtttca gttcgcagtt
     9421 acagtcggct tcaaaagctt ttttgatctt tggaccaggc ccccagtcgg cggcgaagga
     9481 atcgtaggta taaacagtca gaacgggttt agcgaaaacg ggcgctgtgc acagcaacag
     9541 caaggggaga cattttttta acactttgca cctcaaaaaa gagtggcaaa ggacttgaga
     9601 aggagcctca aatcccttcg ccggcgttat ccggatcagg ttcgacgggt attttctcag
     9661 cgcacgcgtc tgcgtggcac cccgttgaga acggcgttag tgtagtgatt ttgttatcaa
     9721 ccagcaatca tggatccggc ggcgcaaacc acgctgattt aaaatcgaac cagccgaggc
     9781 tattcatacg caggccgcgc atactgcgtt gcccctgaat gatcagccag tggtggataa
     9841 gcggcaccat tgctttgctg gcgaccagtt gctggcacca gttcgccagg tttatttcgc
     9901 cattgcgcca gcgggcggcg tcggcttgcc agtcgatcgg aatacagtgt tgtagcagcg
     9961 gcacctcgca caggtgcgcg aatagcgaaa aatccagcgg cagcgtaaag ttggcgctgt
    10021 taagccagat atcgctctcg atctctcctt catgccactg atcgtagctg atctctttga
    10081 tttccagcgt gacctggtga cttgccagaa tctgctgcat gatcccggca atcaccctat
    10141 gctcactgtg atcctgataa aaggtcaggg tgaggctttc cagaccagcc ggtttttcgc
    10201 tctttatggt gcgggtatgg tgccaacggg gaagcagccc ataggccgga aaccacaact
    10261 gctggtactg ttcctcggcg aaataaacca gattggtcgg cgaaagcaca tagcttatcc
    10321 agtctctgac ttgctgattc gccccgcgat gggtacggct gtcgaacagt aaatagtaac
    10381 aaccttcttc aaggcggctt tcaatctctt tttcctcgtc ttgcggtcct ttcagcatca
    10441 ggcctcccgc ggggtcatca gcaatttccg gcaagaccca gacgttaact tcgtcgatta
    10501 atgcccgata accaaagaag tcgtcgaatg cctgaatttt cagttgattg ctgctgttgc
    10561 ggatcaccgc ataaggaccc gttccgatgg gatggctggc aaagttgctg agggtttccc
    10621 attcgcgcgg caggatcatc gccggaactt gccccagcag taacggtaac cagcggtccg
    10681 gttgcgtgag atggatatcc agcgtccagg gcgtcggcga cacaatgtca gcaatatgcg
    10741 aatagagcgg cagcgtattg attcgtttta aagaggcgat cacgtcgtcc atttccagtt
    10801 cacgaccatg atgaaaatgg actcctggac gcaaaaagaa acgccagtga agcggtaaaa
    10861 tttgctgcca gtggtgggcg atgtccgctt ccagttcccc attttcctca tttatgcgcg
    10921 ttagcgaact gaagatttgc cgggcgatat gggtttcgga acgacgcaat gcactgccgg
    10981 gcagcagatt acgcaacgga cgatagtaga gaacgcgcag gatgtgccgc ccctggcgga
    11041 agctgcgtcc cagatgagaa accaacattt gccgcacagc cgctttgtcg ccaaccagtt
    11101 gcaccagttg atcgatacga tcctgctcca gcaggtcctc tgctcgctgt tgctgaagcg
    11161 ccagcccggt atagaggaat gtcagacgcg agcgtttgcc gcgcccgact tccgcttccc
    11221 acgtcagcca gccgcgatcc tgcatggtgt tgagcagggt gcgcatatga cgacgcgagc
    11281 agctcaataa cgctgccagt tcgttgagcg tcgtgtcctg cgatttaccc tcgcagcatt
    11341 gccacaggcg gatgaactgt tgttgcagac gagcagatgg cataaaaggg gaactcctgc
    11401 gcaaaagaca gcaattttat tttccctata ttaagtcaat aattcctaac gatgaagcaa
    11461 ggaggtgcct catgcgtcag ttttatcagc actattttac cgcgacagcg aagttgtgct
    11521 ggttgcgttg gttaagcgtc ccacaacgat taaccatgct tgaagaactg atgcaatggg
    11581 aggggagtca ttctgactac tgatttgcgg acatcatgtg tgactgagta ttggtgttaa
    11641 tcgccacgcc agcagtgatt atctgctggc ttttttcttt ttggcgattg agtgccggac
    11701 ggtgtacaaa ctaacgcata aacgccggtt gcttctcttc ataagacgca attgcgtcgt
    11761 cgtgctgcag agtcaggccg atgctgtcca gaccgttcat catgcagtgg cggcggaagg
    11821 catcgatggt aaagcgataa gttttctctc ccgctttcac ctcttgcgct tccaggtcta
    11881 catcaaaatg aatccccgga ttggcctgca ccagcgcaaa cagttcgtcc acttctgcgt
    11941 cgcttaattt caccggcagc agctggttgt taaagctgtt gccgtagaag atgtcagcaa
    12001 aactcggtgc aatcaccact ttaaaaccgt aatcggtcaa tgcccacggc gcgtgctcac
    12061 gcgaggaacc gcagccgaag ttttctcgtg ccagcaaaat ggaagcgccc tgatactgcg
    12121 ggaagttcag cacgaagtcc gggtttggct gttggccttt ttcatccaga aaacgccagt
    12181 cgttaaacag atgcgcgcca aaacccgtac gggtcacttt ctgcaaaaac tgtttcggga
    12241 tgattgcatc ggtatcgaca ttggcggcat ccagcggaac caccaggcct gtgtgtttga
    12301 taaatttctc tgccatggtg tgctccttat ttaatgttgc gaatgtcggc gaaatgtccg
    12361 gtcacagcag cagcggcagc cattgccggg ctgaccagat gggtgcgccc tccacgcccc
    12421 tggcggcctt caaagttacg gttgctggtg gaggcacaac gctcacccgg attcaggcga
    12481 tcgttgttca tcgccagaca cattgagcaa cctggcaagc gccattcaaa accggcttca
    12541 ataaagattt tgtccagacc ttccgcttcc gcctgggctt ttaccgggcc agagccggga
    12601 accaccagtg cctgcacgcc tggcgcgact ttccgtcctt tggcgatttc agctgccgcg
    12661 cgtaaatctt caatacgtga gttagtgcag gaaccgataa acactttgtc gatagccact
    12721 tctgtcagcg gaatacccgg tttcagcccc atataggcca gcgctttttc tgccgacgcg
    12781 cgttcaaccg gatcggcaaa cgaagccgga tcgggaatgt tgtcgttcac ggaaatcacc
    12841 tgacctggat tggtgcccca ggtgacctgc ggtgaaattt cttctgcctg caaagtcaca
    12901 acggtatcga aagttgcgcc ttcgtcggtt tgcagagttt tccagtaggc aacggcgtcg
    12961 tcgaaatctt tgcctttcgg cgcatgcaga cggcctttga catagttaaa ggtagtttcg
    13021 tccggtgcaa ccagaccggc tttcgcgccc atttcgattg ccatattgca cagggtcata
    13081 cgaccttcca tgcttaaatc acggattgct tcgccgcaaa actccaccac atgcccggtg
    13141 ccgcctgcgc taccagtttt accgataatc gccagcacga tatctttcgc tgtaatgccc
    13201 ggcgcggctt tgccctggac ttcaattttc atggtcttcg cacgaccctg tttcagggtt
    13261 tgcgttgcca gtacgtgttc aacttcggaa gtgccgatac caaaggctag tgcgccaaac
    13321 gcgccgtggg tggcggtatg cgagtcgccg cagacaatgg tcatccccgg caatgtgacg
    13381 ccctgttccg gccccattac gtggacgatc ccctgatacg ggtgattcag gtcatacagc
    13441 tcgacgccaa attctttgca gtttttgatc agctcctgca tctggatgcg cgccatttca
    13501 ccgcaggcat taatgtcttt ggtctgagta gagacgttgt gatccatagt ggcgaaggtt
    13561 ttgcctggct gacgtaccgg gcgaccgtgg gcgcgcagac catcgaacgc ctgcggtgag
    13621 gtcacttcat gcaccaggtg gcggtcgata tataacagcg gggtttcgtt ttcggcttcg
    13681 tacactacgt gagcgtcgaa caatttttcg tataacgttt tagccatgat tacacccctt
    13741 ctaccacata gcgggcaatg atatcgccca tttcattggt actaacggcg gcagcgccac
    13801 gggctaaatc cccggtgcga atgccttctt ctaatgcgcg gttaatggcg cgttcaatgg
    13861 cgcaagccgc atcatctgca tccaggctgt agcgcagcag cagtgccagc gacagaattt
    13921 gcgcaatcgg gttggcgatg tttttgcctg cgatatctgg tgctgagccg cctgccggtt
    13981 catacaaacc aaaaccttgc tcgttcaggc tggcggaagg caacatcccc atcgagccag
    14041 tgatcattgc gcactcgtca gacagaatgt cgccaaacag gttggaacac agcaggacgt
    14101 caaactgtga tggatcttta atcagctgca tggtggcgtt gtcgatgtac atatgcgcca
    14161 gttcgacatc cgggtattcc gtggcgatct cgttgacgat ttcacgccat aaaatagagg
    14221 attgcagcac gttggcttta tcgatcgacg tcactttatg gcgacgttta cgagcagatt
    14281 caaacgcgat gcgggcaata cgctcgatct caaaacggtg atacacctcg gtatcaaacg
    14341 ctttttcata ttgtccgcta ccttcgcggc cttttggctg accgaaatag atgccgccgg
    14401 tcagttcacg cacacacagg atgtcgaagc cgttagcggc aatgtcagca cgcagtggac
    14461 agaatgcttc cagcccctga tacagttttg ccggacgcag gttgctgaat aatttgaagt
    14521 gcttacgcag aggcagcagc gcgccgcgtt ctggttgctg gtctggtggt aaatgttccc
    14581 acttcggacc gcctaccgag ccaaacagca cggcatcggc ttgctcacaa ccttcaaccg
    14641 tcgcaggcgg cagtggttgc ccgtggttat caatggctgc gccgcctaca tcgtaatggc
    14701 tggtggtgat acgcatcgca aatcggttgc gcacggcatc cagcactttc agcgcctgag
    14761 tcatcacttc cggaccaata ccgtcccccg gcaatacggc aatatggtaa ttcttcgaca
    14821 tcacacggtt tccttgttgt tttcgttgtg ttgagctttg cgttgcaact ctttttcgac
    14881 ttctgcggca cgccagatat tgttcagtac gtgcaccatg gctttggcgg aggactcgac
    14941 aatatcggtg gccaggccga cgccgtggaa gcggcgaccg ttgtagttgg cgacaatatc
    15001 cacctgaccc agcgcatctt taccgtgacc tttggcggtc aggctgtatt tcaccagttc
    15061 gacgttatag tcagtgatgc ggtttatcgc ctggtagacg gcatcgaccg gaccgttacc
    15121 gttggcggct tctgctttga cttcttcgcc gcaggccagt ttgacggcgg cagtggcaat
    15181 atcgttagaa cctgactgca cgctgaagta atccagacgg aaatgctccg gctcttcttg
    15241 ctgcttaccg atgaaggcca gcgcctccag atcgtaatca aacacctgac cttttttgtc
    15301 cgccagcttc aggaaggcat cgtacaaatt gtctaaatta tattcacttt ctttataccc
    15361 catctcatcc atgcggtgtt ttactgccgc acgccccgaa cgagaggtca gattcagctg
    15421 gatttggttc aaaccaatag attctggtgt catgatttcg tagttttcgc ggtttttcag
    15481 cacgccatcc tggtggatac cggaggagtg tgcgaatgcg ccgctgccaa caatggcttt
    15541 gtttgccggg atcggcatat tacaaatctg gctaactaac tggctggtgc gccatatctc
    15601 ctggtgatta atagcggtgt ggacgttgag gatatcctta cgaactttga tcgccatgat
    15661 gacttcttcc agcgaacagt taccggcacg ctcgccgatc ccgttcattg caccttccac
    15721 ctggcgtgca ccggcatgta ccgccgccag tgagttgccg accgccaggc ccagatcgtc
    15781 gtgggtatgt acggaaataa tggctttgtc gatgttgggt acgcgttcat acagcccgct
    15841 gatgattccg gcgaactcaa atggcatggt gtagcccacg gtgtccggaa tgttgatggt
    15901 ggtggcaccg gcattaatcg ccgcttcgac cactcgcgcc agatcggcaa ttggggtgcg
    15961 tccggcatct tcgcaagaaa attcaacatc atcggtgtaa ttacgggcgc gtttcaccat
    16021 atagatagcg cgttcgatca cttcgtccag cgtgctgcgc agcttggtgg cgatgtgcat
    16081 tggcgaagtg gcaataaagg tatgaatacg gaaggcttcg gcgactttca gggattcggc
    16141 tgccacgtca atatcttttt ccacgcagcg agctaacgcg catacacggc tatttttaac
    16201 ctggcgagcg atggtctgca ctgattcaaa atcacccggc gaagagacgg ggaaaccgac
    16261 ttccatcacg tcaacaccca tacgctcaag ggccagcgca atttgcagtt tttctttcac
    16321 actcaagctt gcctgtaacg cctgttcacc gtcgcgcaat gtggtatcga aaataatgac
    16381 ttgctggctc atggtttggg tccttgtctc tttttgagcg cctcgcttcg ggcataaaaa
    16441 aacccgcgca atggcgcggg ttttttgttt gactgagtgc tgacttaatt ctgaatgccg
    16501 ctcaccagtc taccgcgcaa agaagatgcg tttagtagta gtagaccgat aaagcgaacg
    16561 atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc ttttagagtt actggatacc
    16621 aaaacggatg tcaaccctga tgcaacaaaa acgtccagcc agcgtgagtt ctgcatccgt
    16681 caaattagct aattgtgctg cggtggttaa aacaagcgat attaatttct gcttaaccac
    16741 cgccgctttt catcggttga catatttcag aataaatttt tgcatctaat cgacgaggga
    16801 aagggggata aaatgcacgc gttacaaaac cta
//
LOCUS       DAZGAP010000121        16698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGAP010000121 DAZGAP010000000
VERSION     DAZGAP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.122"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            86..343
                     /gene="bhsA"
                     /locus_tag="ACHLAM_003404"
                     /gene_synonym="ycfR"
     CDS             86..343
                     /gene="bhsA"
                     /locus_tag="ACHLAM_003404"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO7854876.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(426..1388)
                     /gene="ldtC"
                     /locus_tag="ACHLAM_003405"
     CDS             complement(426..1388)
                     /gene="ldtC"
                     /locus_tag="ACHLAM_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO7854877.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1532..4978)
                     /gene="mfd"
                     /locus_tag="ACHLAM_003406"
     CDS             complement(1532..4978)
                     /gene="mfd"
                     /locus_tag="ACHLAM_003406"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO7854878.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5106..6179)
                     /locus_tag="ACHLAM_003407"
     CDS             complement(5106..6179)
                     /locus_tag="ACHLAM_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO7854879.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMTSLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6441..7640
                     /gene="lolC"
                     /locus_tag="ACHLAM_003408"
     CDS             6441..7640
                     /gene="lolC"
                     /locus_tag="ACHLAM_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO7854880.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7633..8334
                     /gene="lolD"
                     /locus_tag="ACHLAM_003409"
     CDS             7633..8334
                     /gene="lolD"
                     /locus_tag="ACHLAM_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO7854881.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8334..9578
                     /gene="lolE"
                     /locus_tag="ACHLAM_003410"
     CDS             8334..9578
                     /gene="lolE"
                     /locus_tag="ACHLAM_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO7854882.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9607..10518
                     /gene="nagK"
                     /locus_tag="ACHLAM_003411"
     CDS             9607..10518
                     /gene="nagK"
                     /locus_tag="ACHLAM_003411"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO7854883.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10534..11355
                     /gene="cobB"
                     /locus_tag="ACHLAM_003412"
     CDS             10534..11355
                     /gene="cobB"
                     /locus_tag="ACHLAM_003412"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO7854884.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11481..12266)
                     /locus_tag="ACHLAM_003413"
     CDS             complement(11481..12266)
                     /locus_tag="ACHLAM_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO7854885.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12263..12724)
                     /locus_tag="ACHLAM_003414"
     CDS             complement(12263..12724)
                     /locus_tag="ACHLAM_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO7854886.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPYSKKTRRKRKSRP"
     gene            complement(12782..13828)
                     /gene="potD"
                     /locus_tag="ACHLAM_003415"
     CDS             complement(12782..13828)
                     /gene="potD"
                     /locus_tag="ACHLAM_003415"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO7854887.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(13825..14619)
                     /gene="potC"
                     /locus_tag="ACHLAM_003416"
     CDS             complement(13825..14619)
                     /gene="potC"
                     /locus_tag="ACHLAM_003416"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO7854888.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14616..15473)
                     /gene="potB"
                     /locus_tag="ACHLAM_003417"
     CDS             complement(14616..15473)
                     /gene="potB"
                     /locus_tag="ACHLAM_003417"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO7854889.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15457..16593)
                     /gene="potA"
                     /locus_tag="ACHLAM_003418"
     CDS             complement(15457..16593)
                     /gene="potA"
                     /locus_tag="ACHLAM_003418"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO7854890.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPADEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFTVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
BASE COUNT         4051 a         4246 c         4362 g         4039 t
ORIGIN      
        1 atgtgatcca gatcacatct atcatttagt tatcgatcgt taagtaattg cttgcgacgt
       61 cattcatctg cataaggcca ctattatgaa aaacgtaaaa accctcatcg ctgcggcgat
      121 tttaagctca atgtcatttg ccagctttgc ggctgtcgaa gttcagtcaa cgccagaagg
      181 ccaacaaaaa gtcggtacta tcagtgctaa cgcggggaca aatctgggat cgctggaaga
      241 gcagctggcg caaaaagcgg atgagatggg cgcaaaatct ttccgtatta cttctgtaac
      301 cggtccgaat accctccatg gaacagcagt aatttataaa taagcattaa ccctcattaa
      361 tgcctgctac tgctgatttt tttccccgcg acatgccgtg tcgcggggat ttttttatcc
      421 gagctttaca gagtttgtgg gctcacttga tgacggcgga catccaccgg catcccggaa
      481 cggacatcca tcacatgttg catcacttca gcgtcagttt gtgctgcatc tttaaatgat
      541 tgcattgcgc tattcagcgt aattggcagc agctgcggat cgtcatcaat cttctctgac
      601 agcggctgat gtacttcaac cagacgcgca ccgtttggtt cggcagagac ttttatcgga
      661 gtgttgatga tattcacttt ggtgcctggg gtgacctggc taaagagtgt tttgatatcg
      721 tcatcccgca gacgaataca gccagaactt acccgcatgc caatgccgaa atcggcgttc
      781 gtaccgtgga gcaaataaac gccgccatag gccgccaggc gaatcgcatg atggcccatt
      841 ggattatccg gtccagccgg aactaccgca ggcaactcaa ttccctgtgc tttataacgg
      901 gcgcggatgt ttgccgttgg cgtccaggtt gggtttgcac gtttgtctga aacggtggtc
      961 accattgtcg gcgtcagcgt gtcaccacct aactgaccaa tgccgatggg atacacggtt
     1021 accgaatttt tacccggcgg gtagtaatag agacgcagtt ccgcaatatt gatcacaatg
     1081 ccttcgcgcg gcgcatctgg aagtagggtt tgcaacggga tcgttaacac gctgcccgcg
     1141 cgcggtacgt aaggatcaac gccggggtta gcctgtaaca gagcgagaaa gccgacgtta
     1201 tattttttgg cgatggcttc cagagaacca ccgtcatttt ccaccacatg aaatttgttt
     1261 tcgccaacca gacgactgcc cgctggcggc agcggccagg tgttggcttt tgccggtagc
     1321 gccagcgcca cggcagcggc gaacgtaaaa aacgttagcc agcgagaaaa acgcgttttg
     1381 atcatcacca aaaatccata ataaatataa ggttattgta ataaacagat aagcctgaat
     1441 tatggatggt gacagtgtcg gatagtgcag ggaagtgcaa agaatttgta aatgttgcag
     1501 atgggggcgc agaaacgccc ccgatttacc attaagcgat cgcgttctct tccagttcac
     1561 gcataaactg gcgtacccat tcgatacgcg ttttccgctc actcaaatcc tgaataaact
     1621 tcaggcgcgt cggaccatcg aggcgataat gctgcggctg tttttgcagc aaaccaatca
     1681 accaggccgg attaacgtga ttcttctcgg caaattcgat cacgccgcct ttctcattac
     1741 cttccagctt cctgatcccc agtttctgcg cttgctggcg cagacgggca atatccagca
     1801 gggtacgcgc cggatccggc agcaggccga agcgatcgat aagctcgact ttgatctctt
     1861 ccagttcgtt ttccgttttg gcgctggcaa tacgtttgta gaacgacaga cgcgtgttca
     1921 cgtcagggat gaaatcatcg ggcaatagcg acggcatccg cagctcgact tctgtttgct
     1981 gactggtgag atcttccagc gacggctcgc gtccggcttt cagcgcatcg acagcgtttt
     2041 ccagcaactc catatacagc gagaaaccga tggtttccat tgagccgctt tgttcttcgc
     2101 caagcagttc acctgcgccg cggatctcca gatcgtgcgt tgccagcgca aaacctgccc
     2161 cgagatcttc cagcgaggca atcgcttcaa gacgtttttg cgcatcggta gtcatcgctt
     2221 ttggatgcgg cgtcagcagc catgcatatg cctggtggtg cgaacgcccg acgcgaccgc
     2281 gtaactggtg caactgtgcc agaccgaagt gatccgcgcg ttcaatgata atggtgttgg
     2341 cggtaggaat gtcgatcccg gtttcgataa tggtggtaca aaccagcacg ttgaaacgtt
     2401 gatgatggaa atcattcatc acccgttcca gttcgcgctc acgcatttgc ccgtgaccga
     2461 tggcaatccg cgcttcaggt accagttctg ccagccgttc ggcggctttc tggatgtttt
     2521 ccacatcatt gtagagataa taaacctgtc ctccgcgcaa aatttcacgc aggatcgcct
     2581 cccggaccac cagactgtca tactcacgga caaaggtttt aactgccaga cgacgggcgg
     2641 gcggcgtggc gataatcgac aggtcacgca ttccgctcat tgccatattc agcgtacgtg
     2701 ggatcggcgt tgcagtaagc gtcagaatat ccacgttcgc gcgcatcgct ttaatgcgct
     2761 ctttatgacg caccccgaag cggtgttctt catcgacaat cagcaggcct aaatctttaa
     2821 acttgacgtc actttgcagc agtttgtgcg taccgatcag aatatcgatt ttcccttccg
     2881 ctacttccgc aaggatttgc gtctgctctt tggcgctgcg gaaacgggag atcatttcga
     2941 tacgtaccgg ccagttggcg aaacggtcgc ggaagttgtc gtaatgctgc tgcgcgagaa
     3001 gggtggttgg caccagcacc gccacctgct tgtggttatc tactgccagg aaagcggcgc
     3061 gcatcgccac ttctgtttta ccaaagccaa catcgccgca caccagacga tccattgcca
     3121 gcggctgaca catgtcgcta agtaccgcat taatggcctg tgcctgatcc ggcgtggttt
     3181 caaacgggaa gctgtcgcag aacaactgat actgctcgcg atcgtgttta aacgcgaagc
     3241 cctctttggc ggcgcgttgc gcgtagatat ccagcaattc tgctgccaca tcacgcactt
     3301 tttccgccgc tttctgccgc gcgcgtgacc aggcatcgcc gccaagttta tgcagcgggg
     3361 cgttttcttc cgcgccaccc gcgtaacggc taatcagatg cagtgacgac accggaacat
     3421 acagtttggc gtcgttggca taggtgagca tcaggtactc gccagtaata ccgcccgctt
     3481 ccagcgtggt cattccggca tagcgcccga caccgtgctc cagatggacc accggctgac
     3541 caatatgcag ctccgcgagg ttacggatca gtgtatcggg gttgatggcg cggcgagaat
     3601 cctgacgacg acgggcaacg cgttcaccga gcagatcgct ttcgcaaatc agcgccagat
     3661 tacgtaccgt atcgacaaaa ccatgttcgg cagcgccaat catcaaataa cgcccacggt
     3721 cgctggcttc atcaagacgc ataatgcgtt gcggagcaat tttaattcgc gcgagcagtt
     3781 cacccagcgc ttcacggcga ccttcacttt ctaccgagaa caccaccgga ccgtcgaaag
     3841 actcgaggaa cttacgcagc gcatccagcg gtgctttttg ttgtgcctga acggccaggt
     3901 ctggcagttt ctggaaacct aaattggcat tcgcggcttt tgtcggtaaa tgttcagttt
     3961 ttagctgcac acgcggccag tttttcagct ctgaaaagag ctcgtctacc cgcagccaga
     4021 gcgattgtgg tggcaacagt gggcgcatcg gatcgacgcc gcgattttca aaacgcgcca
     4081 gcgtgtcagc ctggaaacgt tcggcactgt tttccagatc gccagtattc accaacagcg
     4141 tattggcagg gaaataactg aacagtggcg gcagcggttc gctgaagaat agcggctgcc
     4201 agtactcgat cccggcaggt aatgtgcctt tactcacttg ctgataaata tgttccggat
     4261 cgcgcttcac ttcgaaggta tcgcgccact ggctgcgaaa cagttcaatt gccgctttat
     4321 cggtcggaaa ttcgtgcgcg ggcagcagat tgatctcttc tacttcctcc agcgtgcgct
     4381 ggctatcgac gtcaaacact cgcaggctgt cgatttcatc atcaaagaaa tcaagacgat
     4441 aaggcagctc actgcccatc gggaagagat ccagcaacgc gccgcgcgtg gcgtattcgc
     4501 cgtgctccat tacctggtca acatggcgat aaccggcgct gtccagttgg gttcgtaatg
     4561 catctcgtga caggcgctga ccttttttca tcaccagcgc atgaccgtgg agaaaactgt
     4621 gcgggcaaac gcgctgcata agcgtattca ccggaacaat cagtacgcca cgctgcatgg
     4681 tcggtagctg gtaaagggtg gaaaggcgcg aggagataat gtcctgatga ggcgaaaaac
     4741 tgtcgtaggg aagggtttcc cagtccgcca gattcatcac catctgatcg gtaaactggc
     4801 tgatttcatc atgcaaacgc agagcatttt gcatatctgg tgcaataagt accaccggac
     4861 cggcgtgacg ttcggcaatt tccgctacca gcgtcgcgca ggcagcgccg gttaactcgc
     4921 ccagcagacg ctgctcaccc gctttgacgg gcagcgtata acgatattgt tcaggcataa
     4981 cggttgtcag attctcgtta ggatatgcct caacatatgg gggcatatct ctgataagca
     5041 atgctgttca ttatccgtaa tcgacatggt ttagcaaatt gaatcgcccc ggctggggcg
     5101 atggcttagc gggagtaact gacggcagcg ggcagtgaag gcggggagaa caacagatcg
     5161 ctgaatgcac gctgtgaaag ttttcttacc agcaagcccg caacagtaca gatgaacaaa
     5221 ctgacaaagg gataaaccat caggagtgtc agttcgactt gcggcgacca gcgtgccgcg
     5281 ttcattggcg caagcagagt taagctgaat atctctacca gaatgcgatg ggtggtgtag
     5341 atagcaatgg tgttggtgcc aatcacattc agcaggctgg tggagcgcat accgaaacgt
     5401 tgctcgtatt gataaaacag cttcatgatc accacaatcg ataccagcga caacagcagc
     5461 gagatagtaa acaaccaggc accgaccgcc agaacggtca gcaaagaagt catcagcaga
     5521 tggcggcgca acggcacctc tttaacgtag gtcattaatg ttgcgccaaa ccatgcgcca
     5581 aggctgtaat aaggcaaatt gcggatcaca ctgttcattc cccaccacgg cgtgggaacg
     5641 aaattaaccg ccacactcat cagtacaaac aaggcgaata gtggcagcgc cagacggcta
     5701 aaaattttac ataccacgaa ataaacaatt aacgcataca gataccacaa actggtgctg
     5761 gcggtgatca tcccgtgcag gaactcaccg gtggaatcgg catacgcggc attggaggca
     5821 ttgcttaaat cgcgctcagg tgccagccac tggttcagcg cacttaacgc cagccactgc
     5881 accacgcccc aaagcgccag cacccagaag atgttccaga tccgtttatc gagacaattt
     5941 ccccacggta cgctgtcgat ataacggcga atcaaatagc cggatataaa gaaaaaaacc
     6001 ggcatacgaa agggtgcaag gtaaagattg aaatagatcc agcatttgct caggacttcc
     6061 gataacggat gctgaaaagt ggtgagatgc ggataaaagg taatgaccga gtgataaatc
     6121 accaccagac aaatacataa ccctttgatc tggttaatcc atagctcttt ttgtttcatc
     6181 aggacaacac gctcttttta tttaacatgc tgttgatcct gaggggccag tcgcgtggtg
     6241 taatgcgtat ctgtctgtat ttggttattt ttcagataaa gatcaggcta caaggccgat
     6301 gagaaaatga ggccctttag ccgattgatt tgctggcact tttcagatta gccctgacga
     6361 tcacttacag ttcagacgtt tacccatccc gctttcgctt atatactcgt gtctttgcta
     6421 acagcaacca gacggatttc atgtaccaac ctgtcgctct atttattggc ctgcgttaca
     6481 tgcgtgggcg tgcagcggat cgcttcggtc gtttcgtctc ctggctttct accatcggca
     6541 ttaccctcgg ggtgatggcg ctggtcacag tattgtcagt gatgaacggc tttgagcgcg
     6601 agctacaaaa caacatcctt ggcctgatgc cacaggcaat tctctcttct gagcatggct
     6661 ctcttaaccc gcagcaactc ccggaaacgg cagtcaaact ggacggcgtt aatcgcgtcg
     6721 cacctattac taccggtgat gtggtactgc aaagcgcgcg cagcgtggcg gtcggggtga
     6781 tgctgggtat cgacccggca caaaaagatc cactaacgcc gtatctggtc aatgtgaaac
     6841 aaactgacct cgagccgggg aaatataatg tcatcctcgg tgaacagctg gcctcacagt
     6901 tgggcgtcaa tcgcggtgat caaatccgcg tgatggtgcc atctgccagc cagttcacgc
     6961 cgatggggcg tattccaagc cagcgcctgt tcaatgtgat tggcactttc gccgccaaca
     7021 gtgaagtcga tggctatgaa atgctggtga atattgagga tgcctcgcgt ctgatgcgtt
     7081 atccggcagg caatattacc ggctggcgtt tgtggctgga tgagccgctg aaagtcgact
     7141 cattaagtca acaaaaactg cctgaaggca gcaaatggca ggactggcgt gaccgtaaag
     7201 gcgagctgtt ccaggccgta cgcatggaaa aaaatatgat gggcttgctg ctgagcctga
     7261 ttgttgccgt agcggcgttt aacattatta cctcgctagg gctaatggtg atggagaagc
     7321 agggcgaagt cgcgatcctg caaacgcaag gcttaactcc gcgacaaatc atgatggtct
     7381 ttatggtgca aggggccagc gccgggatta tcggtgcgat cctcggagcg gcgcttggcg
     7441 cactgcttgc cagccagtta aataatctga tgccgataat cggcgtcctg cttgatggcg
     7501 cggcgctgcc ggtcgctatc gaacctttac aggtcattgt tattgcgctg gtggcgatgg
     7561 ctatcgcgct gctgtctacg ctttaccctt catggcgcgc tgccgccaca caacccgctg
     7621 aggctttacg ttatgaataa gatcctgttg caatgcgaca acctgtgcaa acgctatcag
     7681 gaaggcagtg tgcaaaccga tgtgctgcac aatgtcagtt tcagcgtggg cgaaggtgaa
     7741 atgatggcaa ttgtcggtag ctctggttcc ggtaaaagta ccttgctgca cctgttaggc
     7801 gggctggata cgccaacctc cggcgatgtg atctttaacg gtcagccgat gagcaaactg
     7861 tcgtcggcgg cgaaagcaga actgcgcaac cagaagctgg gctttattta tcagtttcac
     7921 cacctgctgc cggattttac tgccctggaa aacgtggcta tgccgctgct gattggcaag
     7981 aaaaagcccg ctgaaatcaa cagccgtgcg ctggagatgt taaaagcggt ggggctggag
     8041 catcgtgcga atcaccgccc atctgaactt tctggcggcg aacgccagcg cgtggcgatt
     8101 gcccgtgcgc tggtcaataa cccgcggctg gtgctggcgg atgaacctac tggtaacctc
     8161 gatgcacgta acgccgacag catcttccag ttgcttggag aattgaatcg cttgcagggc
     8221 accgccttcc tggtggttac tcacgacctg caactggcaa aacgtatgag ccgccaactg
     8281 gagatgcgtg atggtcgtct gacggcggaa ctgagcctga tgggggcgga gtaatggcga
     8341 tgcctttatc gttattgatt ggcctgcgtt ttagccgcgg acggcggcgc ggcggcatgg
     8401 tgtcgctgat ctccgtcatt tctaccattg gcattgccct tggcgtggcg gtattgatcg
     8461 tcggcttaag cgcgatgaac ggctttgaac gcgaactgaa taaccgcatt ctggcggtgg
     8521 tgccgcatgg cgaaatagag gcggtggatc aaccgtggac taactggcag gaagccctgg
     8581 ataacgtgca aaaagtgcca ggtattgccg ccgctgcgcc gtatatcaat ttcaccggac
     8641 tggtagaaag tggtgccaat ctgcgcgcaa tccaggtgaa gggcgttaac ccgcaacagg
     8701 aacagcgtct gagcgcatta ccctcgtttg ttcaggggga tgcctggcgc aattttaaag
     8761 cgggcgaaca gcaaattatc atcggcaaag gcgtggcgga tgcgctgaaa gtgaagcagg
     8821 gcgattgggt gtcgattatg atccccaact cgaatcccga gcataaactg atgcagccaa
     8881 aacgtgtgcg tttgcacatt gccggtattt tgcagttgag tggtcaactc gatcacagtt
     8941 ttgccatgat cccgctggcg gatgcccagc aatatcttga tatgggttcc agcgtgtcag
     9001 gtattgccct taaaatgacg gatgttttca acgccaataa gctggtacgc gatgcgggtg
     9061 aagtgaccaa cagctatgtt tatattaaaa gctggattgg tacttacggc tatatgtatc
     9121 gcgatatcca gatgatccgc gccattatgt atctggcgat ggtactggtg attggcgtgg
     9181 cctgtttcaa catcgtctcc accttagtga tggcggtgaa agacaagagt ggcgatatcg
     9241 cagtattaag aacgctgggg gcgaaagatg gtttaattcg cgccatcttt gtctggtatg
     9301 gattgctggc agggctgttc ggtagcctgt gtggtgtgat tatcggcgta gttgtttcac
     9361 tgcaacttac cccgattatt gagtggattg aaaagttgat cggtcatcag ttcctctcca
     9421 gcgatatcta ttttattgac ttcctgccat cggaattgca ctggctggac gtcttctacg
     9481 tactggtcac agcattgttg ctgagtcttt tggcaagttg gtatccggcg cgacgcgcca
     9541 gtaatattga ccctgcgcga gtccttagcg gccagtaaag gcagtacatt aaaacaagga
     9601 gcggcaatgt attacgggtt tgatattggt ggaacaaaaa ttgcgcttgg cgtgtttgat
     9661 agcggtcggc agttgcagtg ggaaaagcgg gtgccgacac cgcgtgacag ctatgacgca
     9721 tttttagatg cagtgtgcga gctggtagcc gaagctgacc ggcgttttgg ctgtaaaggt
     9781 tctgtcggca tcggtattcc gggaatgccg gaaacagaag atggtacgct gtatgccgcc
     9841 aatgtccctg ctgccagcgg taaaccgctg cgtgccgacc tgagcgcacg tcttgatcgc
     9901 gatgtacgcc ttgataacga tgccaactgt tttgcccttt ctgaagcatg ggatgatgaa
     9961 tttactcaat atccactggt gatggggttg attctcggca ccggcgttgg cggcgggctg
    10021 attttcaacg gtaaacccat taccggtaaa agctatatta ccggcgagtt tggccatatg
    10081 cgtctgccgg ttgatgcgtt aaccatgatg gggctggatt tcccgttacg ccgctgcggc
    10141 tgtggtcagc atggctgcat tgaaaattat ctgtctggtc gcggttttgc gtggctgtat
    10201 caacactatt atcatcaacc gttgcaggct cccgaaatta ttgcgcttta tgatcaaggt
    10261 gatgagcagg caagggcgca cgttgagcgt tatctggatt tattagcggt ttgtctggga
    10321 aatatcctga ccattgttga ccctgacctg gtcgtcattg gtggtggctt atcgaatttc
    10381 ccggcaatca caacgcaact ggcggacagg ctgcctcgtc atctcttacc tgtagctcgt
    10441 gttccgcgca ttgaacgcgc gcgccacggt gatgcgggag gaatgcgtgg tgcggccttc
    10501 ctacatctaa ccgattaaac aacagaggtt gctatgctgt cgcgtcgggg tcatcggtta
    10561 agtcgttttc gtaaaaataa acgccgtctg cgcgagcgtt tgcgtcagcg tatttttttc
    10621 agagataaag tggtgccgga agcaatggaa aaaccaagag tactcgtact gacaggggca
    10681 gggatttctg cggaatcagg tattcgtacc tttcgcgccg cagatggcct gtgggaagaa
    10741 catcgggttg aagatgtggc aacgccggaa ggtttcgatc gcgatcctga actggtgcaa
    10801 acgttttata acgcccgtcg tcgacagctg cagcagccag aaattcagcc taacgccgcg
    10861 catcttgcgc tggcgaaact gcaagacgcc ctcggcgatc gctttttgct ggtgacgcag
    10921 aatatcgaca acctgcatga acgcgcaggt aataccaatg tcattcatat gcatggggaa
    10981 ctgctgaaag ttcgctgttc gcaaagtggt caggttctcg actggaccgg agatgttacc
    11041 ccagaagata aatgccattg ttgccagttc ccggcaccct tgcgcccgca cgtagtgtgg
    11101 tttggcgaaa tgccactcgg catggatgaa atttatatgg cgttgtcgat ggccgatatt
    11161 ttcattgcta ttggtacttc ggggcatgtt tatccggcgg ctgggtttgt tcacgaagcg
    11221 aaactgcatg gcgcgcacac cgtggaactg aatcttgaac cgagtcaggt tggtaatgaa
    11281 tttgccgaga aatattacgg tccggcaagc caggtggtgc cagagtttgt tgaaaagttg
    11341 ctgaagggat tataaagcgg gaaaaattgc ctgatgcgct acgcttgtca ggcctgtgaa
    11401 ggggcgcaat taattgaatt tgcgggtttt gtgggccgga taaggcgttc gcgccgcatc
    11461 cggcaacttt actgcctctt ttaccgttta ctcgtccata gtaacgcggc aataaacgta
    11521 cagacaccaa tcagccctgc ccagagcggc gatattctgg taatcgcaaa gctgttatcg
    11581 atatacaaac ataaagcctg atagcccata ttggcaacag ccatagccac tgcaaatttt
    11641 aacgcataat aaaggcgcgt tgtgtagtta aaaaatgcag caaagacgat gaatgcaact
    11701 gcccaggcaa aaaggcctaa tgtcgtggta ctgccgggaa gatcattctc ctgctgttgt
    11761 ccgggatgcg gataatatgt cagtggcggc gtcggttgtt ttaatatctg tgcttgtaaa
    11821 acagccaatg cattattgat tccctgatac cagagatctg catgtaaata agccagagtg
    11881 tgattttcta ccagaaggcc gagatgttta tcgtccatgc tgctttcaac ctggccgttg
    11941 gttatcataa ttaaccgtgg ctcgcgggta gtaaaaagta acagaacact tccttttaca
    12001 ttactcattg aagatgccgc atgcgccagc atcgctttgc cattttgcct gaagcttgct
    12061 tcattaacgc caggtacaac cacgataaac ggcgcaatcc cggtttgttg acgaaaaagt
    12121 tcggctttac gcgcgatctc ttcggcatcc ggtacagtac cggtaacatc cgtaaaaaga
    12181 gtcttcatta ttggaatcaa tggcttactg gttaccgtca gaggcaggag tatcagtaaa
    12241 ctgagcaata ttatgatttt tttcatgggc gggacttccg tttcctgcgt gtctttttag
    12301 agtagggcat actgattaat aaaccgacgg agataaaaat aacgcccata atgccgataa
    12361 acccccattt aaaaaaatta acccacgggt tatttatctc agccttttca ggatgtcctg
    12421 gagaataata aacattaact ttatcgcctt cactgtcttc ataaaaatcg ctgccaatac
    12481 ttgaattgaa aattagcgtg taatctggcg tcggacgaaa ggcaacgacg ggataccacg
    12541 aaccattttt acccgtgcga tgagaatgac tggagtgcca gacggtatca acaatgacgc
    12601 cttcagtatg aatcgcgtct ttgctatagg tgaactggtt ataaaagata acaacagaaa
    12661 tgaggatcag taccagaccg ataccgaaga atatacgaaa gaaaactcta ctgtcctggc
    12721 tcattagcca catccttgct aaataaaaaa cgggcggtaa taccaccgcc cgcttgctga
    12781 attaacgtcc tgctttcagc ttctgataat actcttcata aatgctgctg gctgcgccaa
    12841 cgtcattctg ccattcacca tttttaatgg tttcagcatc cgggtagagc gttttatcgt
    12901 tcgccacttc cggacttaac agcttacgcg ccgcaaggtt tggcgttgga taaccgatag
    12961 tttcagcaac ctgttttgcc acatccgggc gcagcaggaa gttgatcaat ttcagtgcgc
    13021 cttctttgtt tttggcattt gccgggatcg ccaggctgtc catccagaaa atgccgcctt
    13081 ctttcggcca caccacatca attggcgtac ccgcctggcg tgcaacgaaa gcagaaccgt
    13141 tccagatcat gccgaggtta acttcgcctt ccatatacgg gttagcgggg ttatcggagt
    13201 taaacgccgc cacgtttggc atcagttttt tcagctcgtt gtatgcagct tcaatctctt
    13261 tcggatcggt ggtgttaccg gagtagccca gcttacgcag cgccatctgg aacacttcac
    13321 gggcatcgtc ggtcagcagc aggctgcctt tgtactcagg cttccacaga tcggcccagc
    13381 tggtgacaga tttcggatcc accgcatcac cgttaacgcc aatcgccgtt gcaccccaga
    13441 tatacggaat ggaatagtcg ttattcgggt caaacggctt gttgagcatg tctggatcga
    13501 ggttgctgaa attgcttaac ttcgacttgt cgatcttctg gatcatccct tctttacgca
    13561 ttttatcgac gtaataggtt gaaggaacca ccaggtcata ggcaccgtct ttgtacgttt
    13621 tcagcttcgc gtacatggtt tcgttcgact cgtaagtcga atagataacc ttaataccgg
    13681 tttctttggt gaactgttca agcagtcctg gcggcacgta ctcggtccag ttgtagaaat
    13741 acagcgtgtt gttgtcatcg gcgtgagcgg cgctcatgcc cagtgccaga gcacccgccg
    13801 cgagcaggtg gcgtgaccat tttttcattt aacgtcccct gtgttacctt tcgttttatc
    13861 acgagcaata agctggctgg caattaccat caccagcgac agcaccagta atatggttgc
    13921 cagcgcgtta acttccggcg atacgccgac tttgaccatc gaataaattt ttaacggcag
    13981 aatttcataa ctcggcccgg tgacaaacga agaaaccacc acgtcgtcca tcgacagggt
    14041 aaagcttaac acccagcccg ccgctaccgc tggcattgcc agaggcagaa tgatttttcg
    14101 cagaatggta aattcgctgg caccgagatc tttcgccgct tccagcattc gcacgtcaaa
    14161 acctttcagg cgcgaataca ctgtcaccac cacaaatggc aggcagaagg tgatatgcga
    14221 gaacagcagc gaccagaagc caagctgaat acccagcagc ataaacagca ccagcagcga
    14281 aatcgccatc acgatatctg gtgacatcat caccacaaac agcattccgc taacgaacgg
    14341 cttaccacga aagcgataac ggtacagcgc aactgctgtc agtgaaccga taagcgtagc
    14401 aaacgtcgcc gaaaacaccg ccattgttag tgaatgctgc gctgcctgta acaggctgtc
    14461 gttgttcatc agcaggctat accatttggt ggtaaaaccc tgccagttga tgccaaagcg
    14521 cgagctgtta aaggagttca caatcaaaat aatgattggg atatacaggt acgcgtagat
    14581 agcggtcata aaaccgccgc gaagcagtcg accgatcatt cgagttccac cttcttattc
    14641 agcagacgag aagcgcgcca gtaaaccagc aacatcaggc ccattacgat agtcagcgta
    14701 atgctggtgg ctgcaccaaa cggccagtca cgaatattga ggaactgtac cttgatgacg
    14761 ttaccgatca gcaggttttt cgcaccgccc atcaggtcgg acacatagaa caggcccatc
    14821 gctggcagca tcaccagcag acatccggca ataattcccg gcatcgttag tgggataatg
    14881 atgcggataa aggtctgcaa cttgctggca ccgagatcgc gcgccgcctc aagcaacggc
    14941 ttatccagtt tttcgatact ggagtacagc ggcatcacca taaacggcag cagaatgtaa
    15001 accagaccga taatcaccgc actaggcgtg aacatgatgc gaattggtgt atcgataacg
    15061 cccagccaga gcaaaaactc gttgagatag cctttggtgc tgaggaaaat tttcagcccg
    15121 taaatacgaa tcaatgagtt ggtccagaac ggaacaatca gcagaaacag cagcagcgga
    15181 cgcaccttgt gtggcagctt cgccagaaac caggcaaaag gatagcccag caccaggcag
    15241 gcgagggtgg cgatcagcgc catattcagc gagtgcaata gcacttcaaa atagagcgga
    15301 tcgagcagac gcgtgtagtt atccagcgta aagaccattt tgacgaaact ggcgtcgtcg
    15361 cgggtcaaaa agctggtgcc aatgatcatc aggttgggca gaaagacaaa caacacaagc
    15421 caaccgacaa tagtgacaat cactacattc tggaacttac ttgtgttctt catcagccag
    15481 tacgacctcc cagctttcta cccaattaat ggccattttt tggtcgagag agtggtcaaa
    15541 gtcaggatcg tcttcattga agaattcgct gaccatcacc atcttgccat tttccagctc
    15601 aacaaccgac tccagcgtca tgcctttgta gttacgctcg cgaacgtaac caatcagccc
    15661 ttcggcgtgg ttgtcgtcgt taatctcttc aacacgtaag tcttccgggc gcagcagaac
    15721 atgcagtttt tgccccggtt caacggtgaa gttaacgtag atattacatt cgcggccttc
    15781 aacgttggcg cgtacgcgct gctcgtcgag acgttcgatg acagtggcgt taaacatatt
    15841 gatttcgcca ataaacccgg caacaaacag gtttttcggc tcttcgtaga tttcacgcgg
    15901 cgtgccgtct tgctcaatgc gaccatcgcg catcaccacg atcctgtccg acatggtaag
    15961 tgcttcttcc tggtcgtgag ttacgaacac gaatgtaata ccaagcttac gctgtaacgc
    16021 tttcagctcg ttctgcattt gcttgcgcag tttgtaatcc agcgctgaga gcgactcatc
    16081 cagcaacaac agacgaggct tgttaaccac cgcgcgggca atggcgacgc gttgctgttg
    16141 accaccagag agctgatgcg gtttgcgttg agcgaaggtt tccagctgca ccatccgcag
    16201 ggcttccatc acgcggggcg taatttcatc agcgggggtt ttttgcatgc gcaacccaaa
    16261 ggccacattt tcgaacacgg tcatgtgggg gaaaagtgcg tagctttgga aaacagtgtt
    16321 cacgtagcgg ttttccgccg gaacgtgggt gatgtcctcg ttatccaaca tgatgcgtcc
    16381 ggaatcaaca gtttccagac ctgcaatcag gcgaagaacg gttgttttac cgcagccaga
    16441 agggccaagc agcgtgagga actcgccatt gttgatagtc agatccagct ggggaatgac
    16501 ctctttacca tcaaagcatt tgcgaattcc cgccaattgc accagcggtg aaagcgaact
    16561 cggttgttta ttcaattttt tactctgtcc catgtaaacg caacggatgg cttaccgatg
    16621 cggggtttgt ggttaaccac cttggtgact cttaatgagg gcggtaattc tacggcaaac
    16681 cgcttgaatc gccaatct
//
LOCUS       DAZGAP010000122        16565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGAP010000122 DAZGAP010000000
VERSION     DAZGAP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.123"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            212..364
                     /gene="mokC"
                     /locus_tag="ACHLAM_003419"
     CDS             212..364
                     /gene="mokC"
                     /locus_tag="ACHLAM_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO7854891.1"
                     /translation="MKQPKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(468..1598)
                     /gene="dnaJ"
                     /locus_tag="ACHLAM_003420"
     CDS             complement(468..1598)
                     /gene="dnaJ"
                     /locus_tag="ACHLAM_003420"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO7854892.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1687..3603)
                     /gene="dnaK"
                     /locus_tag="ACHLAM_003421"
     CDS             complement(1687..3603)
                     /gene="dnaK"
                     /locus_tag="ACHLAM_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO7854893.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEVGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3979..4383
                     /gene="yaaI"
                     /locus_tag="ACHLAM_003422"
     CDS             3979..4383
                     /gene="yaaI"
                     /locus_tag="ACHLAM_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO7854894.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4409..5122
                     /gene="msyB"
                     /locus_tag="ACHLAM_003423"
     CDS             4409..5122
                     /gene="msyB"
                     /locus_tag="ACHLAM_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7854895.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5271..5837
                     /gene="satP"
                     /locus_tag="ACHLAM_003424"
     CDS             5271..5837
                     /gene="satP"
                     /locus_tag="ACHLAM_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO7854896.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5872..6459)
                     /gene="mog"
                     /locus_tag="ACHLAM_003425"
     CDS             complement(5872..6459)
                     /gene="mog"
                     /locus_tag="ACHLAM_003425"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO7854897.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(6574..7527)
                     /gene="tal"
                     /locus_tag="ACHLAM_003426"
     CDS             complement(6574..7527)
                     /gene="tal"
                     /locus_tag="ACHLAM_003426"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7854898.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7806..9236
                     /gene="yaaJ"
                     /locus_tag="ACHLAM_003427"
     CDS             7806..9236
                     /gene="yaaJ"
                     /locus_tag="ACHLAM_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO7854899.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9306..10082
                     /gene="yaaA"
                     /locus_tag="ACHLAM_003428"
     CDS             9306..10082
                     /gene="yaaA"
                     /locus_tag="ACHLAM_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO7854900.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10122..10418)
                     /locus_tag="ACHLAM_003429"
     CDS             complement(10122..10418)
                     /locus_tag="ACHLAM_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO7854901.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEMTLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10632..11918)
                     /gene="thrC"
                     /locus_tag="ACHLAM_003430"
     CDS             complement(10632..11918)
                     /gene="thrC"
                     /locus_tag="ACHLAM_003430"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO7854902.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11919..12851)
                     /gene="thrB"
                     /locus_tag="ACHLAM_003431"
     CDS             complement(11919..12851)
                     /gene="thrB"
                     /locus_tag="ACHLAM_003431"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO7854903.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12853..15315)
                     /gene="thrA"
                     /locus_tag="ACHLAM_003432"
     CDS             complement(12853..15315)
                     /gene="thrA"
                     /locus_tag="ACHLAM_003432"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO7854904.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15341..15460)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15396..15461)
                     /gene="thrL"
                     /locus_tag="ACHLAM_003433"
     CDS             complement(15396..15461)
                     /gene="thrL"
                     /locus_tag="ACHLAM_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO7854905.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15675..16361)
                     /gene="yjtD"
                     /locus_tag="ACHLAM_003434"
     CDS             complement(15675..16361)
                     /gene="yjtD"
                     /locus_tag="ACHLAM_003434"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7854906.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3846 a         4425 c         4089 g         4205 t
ORIGIN      
        1 gcgtcttcag gattagaatc gataaaacag aatgggttaa agagagcgat ataatagcgg
       61 cgggtgcttg aggctgtctg tctcaggcat tagctgaacg gcagatagag aaaagccccg
      121 agtgatattt tactatcaac ccgaggcccc ctatatgcag aacacatgta gagtgcctct
      181 tactgaccgt aaggtcaagg agaagagagc aatgaagcag cctaaggcga tgattgtcgc
      241 cctgatcgtc atctgtatca ccgccgtagt ggcggcgctg gtaacgagaa aagacctctg
      301 tgaggttcac atccgaactg gccagacgga ggttgctgtt ttcacggctt acgaatccga
      361 gtaagagcaa cggcggggag tgatccccgc cacttttcag gtgctcgcat atcttcaacg
      421 caccctattt ttacccaggc ctgcccacgg gcaggctttt ggggagatta gcgggtcagg
      481 tcgtcaaaaa acttcttcac accatcaaag aagctctttg agcgcgggct gttgtgctcg
      541 ccggttgggc caccgaagct ttcttgcagc tcttgcagca gctgtttctg cttctcgttc
      601 aggcctaccg gtgtttcgac gacaacgcgg cacagcaaat caccctgtgc gccaccgcgg
      661 acagacttga cgcctttacc gcgcatacgg aacagcttac cggtctgggt ttcgccaggc
      721 actttcagtt tgacgcgacc atcaagggtc ggtacttcga tttcgccacc cagcgccgcc
      781 atagcgaagt tgatcgggac ttcgcaatag aggttgttgc cttcacgctc gaaaatcggg
      841 tgctgtttaa cctgaacctg aacgtacaga tcgcctgccg gtgcgccatg ctcgcccgct
      901 tcaccttcgc ccgcaagacg gatgcggtct ccagtgtcca cccctgccgg gattttaacg
      961 gacagcgttt tgctgcgctc aacacgacca tgaccatgac atttgttgca cggatctttg
     1021 atcagcgtac cgcggccctg acagtgtgga caggtctgct gcacggcaaa gaaaccctgg
     1081 cgcatctgca cctgaccaga accatgacag gtcggacagg tctgcggctg tgtacctggt
     1141 tttgcaccgc taccgtggca aacgtcacac tcttccagag tcggaatgcg gatctctttg
     1201 gtcacgccac gtacagcttc ttcgagggtg agctccatgt tatagcgtaa atcagcaccg
     1261 cgcgccgcac gttgacgacc acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa
     1321 atatcgctga agtctgcgcc gccgccgaaa ccgccgcctc ccatgccacc ttgctcaaac
     1381 gcagcatgac catactgatc gtatgccgca cgtttttgcg agtcggtcag aacttcataa
     1441 gcttccttga tctctttaaa tttcgcctcg gcctctttgt caccctggtt acggtccggg
     1501 tggtatttca tggccaggcg tttgtaggcc tttttgattt cacgctcttc cgctgttttg
     1561 gaaacgccta aaatctcgta ataatcttgc ttagccatct tttttaaatt gcccctagat
     1621 gaatgcacgg gcggagagga aattcccctt cgcccgtgtc agtataatta cccgtttata
     1681 gggcgattat tttttgtctt tgacttcttc aaattcagcg tcgacaacat cgtcatcttt
     1741 cgcgttgttt gcagaagcat cagcaccggc agtctgctgc tgggcatgtt gctgctgggc
     1801 gatttccatc agtttctggg aaacctgtgc cagttcctgc attttcgctt cgatagcggc
     1861 tttgtcttca cctttcagag cagtttccag tgcagtcagt gcagactcga tagcagtttt
     1921 gtcgtcagcc ggcagtttgt cgcccacttc ttcaacctgc ttacgggtgc tgtgcagcag
     1981 atggtcgccc tggttgcgag tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt
     2041 agcttctgcg tcgcgtacca ttttctggat ttcatcttcg ttcagaccag aagaagcctt
     2101 gatggtgatc ttctgctctt taccgctgtt tttatctttc gcggaaacgt gcaggatacc
     2161 gtcagcatcg atatcgaagg taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat
     2221 accatccagg ttgaactgac ccagagattt gttatcagct gcacgtttac gttcaccctg
     2281 cagcacatgg atggttaccg cagactggtt gtcttcagcg gtagagaaca cctggctgtg
     2341 cttggtcggg atagtggtgt ttttcgcgat cagcgtcgtc atcacaccgc ccatggtttc
     2401 gatacccaga gacagcgggg taacgtccag cagcagtacg tcttttacgt caccagtcag
     2461 aacaccaccc tgaacagcag caccgattgc tacagcttcg tccgggttaa cgtctttacg
     2521 cggctcttta ccaaagaact cagcaacttt cttctgaacc attggcatac gagtctgacc
     2581 accaacgagg ataacgtcgt cgatatcaga tacggacagg ccagcgtcct gcagtgcaac
     2641 tttcagcggc tcaatggaac ggtttaccag atcttcaacc aggctttcca gtttcgcacg
     2701 agtcactttg atgttcatgt gtttcggacc ggtcgcgtct gcagtgatgt acggcaggtt
     2761 aacgtcggtc tgctgagcgg aagacagttc gattttcgct ttttctgccg cttctttcag
     2821 gcgctgcatt gccagcggat cgttgcgcag gtcaatgccc tgatctttct tgaattcttc
     2881 aaccaggtag ttgatcagac ggctgtcgaa gtcttcacca cccaggtggg tatcaccgtt
     2941 ggttgccaga acttcgaagg ttttttcgcc gtcaacttcg tcgatttcga taatagaaat
     3001 atcgaaagta ccaccaccca ggtcataaac cgcgatagta cggttgccag tacctttgtc
     3061 cagaccgtaa gccagcgcag ctgcggtcgg ttcgttgatg atacgtttta cttccagacc
     3121 agcgatacgg cctgcgtctt tggttgcctg acgctgagca tcgttaaagt atgccggtac
     3181 ggtgataaca gcttcagtta ccggttcacc caggtaatct tcagcggttt tcttcatttt
     3241 tttcagcact tcagcagaga tctgcggcgg tgccattttc tggcctttaa cttcgaccca
     3301 tgcgtcgccg ttatcagcag caataatttt gaacggcatg atggaaacat cacgctgtac
     3361 ttcttcgtcc tggaagcggc ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg
     3421 gttcgtcact gcctgacgtt tagccggctg accaaccaga gtttcaccat cctgggtata
     3481 ggcaatgata gaaggcgtgg tgcgatcgcc ttcggcgttc tccagtacac gaggagtggt
     3541 gccatccata atcgctacac aagagttggt agtacccagg tcgataccaa ttattttacc
     3601 catctaaacg tctccactat atattcggtc atcatgtggt tgtgagtctg taataggggc
     3661 gaaacgtctg gtttcaactg cccaatttca tttttttgca gactcactca ctgcggttga
     3721 ctactaaatg gggtcgtaac ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt
     3781 ttgcgcagaa atttcgcgat atgccagaac aagacatgaa actgacagca attttattca
     3841 tatagagaat gaatagcaga caagacattc atagaaaaat tgtaataaaa agaaaatatt
     3901 acgtacctga atcttctctt tatcagcagt aaactagtgg gtattcatcc cctacctctc
     3961 cccactaaga gaatccttat gaaatccgtt tttacgcttt ccgccagcct ggcgattagc
     4021 ctgctgctgt gctgcacggc gcaggcaaac gaccataaaa tcctcggcgt cattgcaatg
     4081 ccacgtaacg aaaccaacga tctggcgctg aaacttcctg tttgccgaat tgttaaacgc
     4141 atacaactct cagccgacca tggcgattta cagttaagcg gtgcatcggt ttatttcaaa
     4201 gccgcccgta gcgccagtca gaccctgaat attccttcag aaataaaaga agggcaaacc
     4261 actgactgga tcaacattaa cagcgataac gacaataaac gctgcgtctc aaaaatcacc
     4321 ttttctggtc atacggtgaa ctcatcggat atggccacgc tgaaaattat cggcgacgac
     4381 taacgccaga atatagaagc cacaaaaaat gaatgttaat tacctgaatg attcagatct
     4441 ggattttctc cagcattgta gtgaggaaca actggcaaat ttcgcccggc tgctcaccca
     4501 taatgaaaaa ggcaaaactc gcctctccag cgtactgatg cgcaacgaac tgtttaaatc
     4561 gatggaagga catcccgagc aacaccgccg caactggcag ctgattgccg gagaattaca
     4621 acattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat tgtatcgggc
     4681 cattttgctc gatgtttcaa aacgattgaa gctgaaagcc gacaaagaga tgtctacgtt
     4741 tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga aaatggacga
     4801 ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc tggaagagct
     4861 gctgccgctg ctgatgaaag acaaattatt ggcaaaaggc gtgtcgcatt tgctttccag
     4921 ccaattgacc cgcattttac gcacccacgc agcaatgagc gtacttgggc atggtttgct
     4981 tcgcggcgcg gggctgggag gccctgtagg cgcggcacta aatggggtta aagcggtcag
     5041 cggcagtgcc tatcgcgtga caattccagc cgtactgcaa atcgcctgcc tgcgccggat
     5101 ggttagtgcc actcaggtct gataccggaa accggggaaa attttcattt tcccctcaaa
     5161 agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa cactacagtt
     5221 attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt atgggcaaca
     5281 ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc accattctgc
     5341 ttaacctgca caacgtgggt tattttgctc tggacggtat tattcttgcc atgggcattt
     5401 tctacggcgg catcgcgcaa atttttgctg gtctgctgga gtacaaaaaa ggcaacactt
     5461 tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt gcgattctgc
     5521 tgatgccgaa actgggtctg accgatgcgc caaatgcaca gttccttggt gtctacctgg
     5581 gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac gctgaaaggc gcacgcgttc
     5641 tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc ggtaacattg
     5701 ccggtaacgc cgcaatcatc cactttgccg gctggattgg tctgatctgc ggtgccagcg
     5761 caatctatct ggcgatgggt gaagtactga acgagcagtt tggtcgcacc gttctgccga
     5821 ttggtgaatc ccattaattg ttttgagatc cccccgctcg gggggatttt tttattcgct
     5881 aacgtcgcgt cttgcactct tcggtctgaa tgctgcaacc acttccggtg ccgtttcaat
     5941 gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac catgcaccac
     6001 aacgttaccc gcagcgtcct tcacgccttc cagcgtctct ttaatcgact tcggctgacc
     6061 gggtaagtta aggatcagcg cctgtttgcg aatcacgccc acctgacgcg aaaggatcgc
     6121 agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagccag gcatctcgcg
     6181 gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgccgggc cagttccgcc
     6241 cgtggtgagc accagatggc aactcatttc atccaccagc tcacacaacg tttgctcgat
     6301 gatcgcctgc tcatcgggga ttaagcgggt ttccagttca aacggcgtgg ttagcgccga
     6361 tgtcagccat tcttccagcg cagggatgcc tttatcctga taaacgccgc tggatgcgcg
     6421 atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc cgtttaaaca
     6481 gtctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggcttc agaaaaggta
     6541 gcgtgaccga cttcccggtc acgctaagaa tgattacagc agatcgccga tcattttttc
     6601 cagtttttcc tggtcaacag caaacttacg gataccttcc gccagtttat ctactgccat
     6661 tggatcctgg ttatgctgcc acaggaactc ggactcagtg atacgcgccg gacgcgcttt
     6721 cacttcgccg gtgtaagaca atttacgttc gatagcccct tcgctctcag ccagctcttt
     6781 cagcagcgcc ggtgcgatgg tcagacggtc gcagcctgcc agttccagaa tttcgccgat
     6841 gttacggaag cttgcgccca tcaccacggt ttcataaccg tgctctttgt agtactggta
     6901 gatttcagat acagaaacca cgcccggatc ttctgccgga gcgtactctt tcttgtcggt
     6961 attcgctttg taccagtcaa gaatacggcc aacaaacggc gagatcagga acacgcccgc
     7021 ttccgcacaa gcacgagcct gagcgaagga gaacagcagg gtcaggttac agttaatacc
     7081 ttctttttcc agctgttctg cagcacggat accctgccag gtagaagcca gtttgatcag
     7141 aatacgatcg ttgctaatcc ctgcatcgtt gtagagtttg atcaggcgtt ttgctttcgc
     7201 aattgacgct tcggtgtcat aggaaagacg cgcatcaact tcagttgaga tacggcccgg
     7261 aaccagtttc aggatttcca gaccaatatt tactgccagt ttgtcggtcg cgtccacgat
     7321 ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat caatcaactt
     7381 acggtattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg tggcatcctg
     7441 cggttgatac agcttcattg ccgcgatgtc cccagtgtcg gccactacgg tggtgtactg
     7501 acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc ttgttagggg
     7561 gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc gccgacaaga
     7621 cggttatcgc caggcgagac tgtttcggat ttctgacact gcgttgatat cgcccgccat
     7681 ttttatacaa aacctcatgt atgctacgca gaagttatca agtacctcgt agcgtatata
     7741 cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca ataagaggga
     7801 tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg gtaatgattt
     7861 acctgctctt cggcgcaggt tgttggttca cttttcgcac cggatttgtg cagtttcgct
     7921 acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca ggcggtttaa
     7981 cctcatttca gtcattgtgt accagtcttg cggcgcgcgt gggtagcggc aacctggctg
     8041 gcgttgcgct ggctattacc accggtggac caggtgccgt cttctggatg tgggttgccg
     8101 cgtttatcgg catggcgacc tcgtttgccg aatgttccct tgcacaactt tataaagaac
     8161 gtgacgttaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc gggctgggga
     8221 tgcgctggat gggcgttctg ttcgccctct ttttgctcat cgcctacggc ataattttca
     8281 gcggagttca ggcgaacgcc gttgcccgag ccctgagttt ttcttttgat tttcccccgc
     8341 tggtgacagg cattattctt gctgtctttg ctctgctggc aatcactcgc ggtcttcatg
     8401 gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc gataatctgg gtactgacca
     8461 gcctggtaat atgcgtaatg aatatcgggc aacttcccca cgtcatttgg tctatttttg
     8521 agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc ttaagccagg
     8581 cgattactaa cggttttcag cgcagtatgt tttccaatga ggcgggaatg gggtcgacgc
     8641 caaacgcggc agcggcggct gcgtcctggc ctccgcatcc cgcagcgcaa ggaattgtcc
     8701 agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc atgctgatat
     8761 tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt atccagaagg
     8821 cgatgcgggt actcatgggt tcctggggtg ctgagtttgt taccctcgtg gttattctgt
     8881 ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc ttcttcttac
     8941 gcctgaacaa ccctaaagcg atctggtgtc tgcggatctg caccttcgca acggtcatcg
     9001 gcggcacctt gctaagtctt ccgctgatgt ggcaactggc agatatcata atggcctgca
     9061 tggctattac caatttgacc gccatattac tgctctcgcc tgtggttcat accattgcca
     9121 gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc
     9181 cggatatcgg tcgccagctt tctccggacg cgtgggatga tgtttcgcag gagtaatcac
     9241 aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc ctgcaaggac
     9301 tggatatgct gattcttatt tcacctgcga aaacgctcga ttaccaaagc ccgttgccca
     9361 ccacgcgcta tacgctgccg gagctgttag acaattccca gcagttgatc catgaagcgc
     9421 ggaaactgac gcctccgcag attagcacgc tgatgcgcat cagcgacaaa ctggcgggta
     9481 tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggagaat gcccgccagg
     9541 cgattctggc attcaaaggt gatgtctaca ccggcttgca ggccgaaacc ttcagcgaag
     9601 acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat ggcgtactgc
     9661 gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt gagaatgccc
     9721 gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg aacgaggcgc
     9781 tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat tttaaatcgg
     9841 tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat gagaagaacg
     9901 gcaaatttaa gatcatcagc ttctacgcta agaaagcacg cggtctgatg agtcgtttca
     9961 ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc gaaggttact
    10021 tctttgatga agcttcctcc agcaacggcg aactggtgtt taaacgctac gagcagcgtt
    10081 aagtttgcag tcaatgccgg atggcaatcc ggcattgata tttagcgatg atgtttgtca
    10141 ggccccggac gatgatcgcc acgatgatca ttgtgcttgt tatggcgcgg cgatggcggc
    10201 ggtccatatg ggtgccagcg attgcctcgc cattcataat gttgtttcca ccagccgtgg
    10261 tcgcgccagt gaccgccatc ccagtaatag ccacgattat cacgatcgcc tatctgtaat
    10321 tttactgacg ggactaacgt catttccgca gcctgcgcag ccatgggagc gaccagaacc
    10381 agggaaagtg cgagtacgat agattgcatc tttttcacag gttactcctt ttatcattgc
    10441 ccgttatggg tcgatatccg gagattacgc gatgagaacg gcagttgtta ttctccgcaa
    10501 tcctaatctc taagttaccg catttattga gcatttctcc tttttccctg tcgtttttct
    10561 ccataatttc ttcataaaaa agccgggctg cataaaagca aacccggcct gattgagata
    10621 atgaatagat attactgatg attcatcatc aatttacgca acgcagcaaa atcggcgggc
    10681 agattatgtg aaagcaaggg taaatcggca cgttctgcca gctcttttgg cagatccaac
    10741 gtttcaccga gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg
    10801 aacaagccat attcgcctgg atgcaactgg tcacgcagtg cacgatacgc tacggcagcg
    10861 tgcggctccg aagtgtagcc cagttctttt aactcacgca ttgtctgttg cgtggtttca
    10921 tcatccacgg ctgcataacc cagctctttc agttgccaga ttttgcggcg gaacaactct
    10981 tccacacgcg gccagttgtt cggctgactc acgtccatcg cgttggataa cgtcgcctga
    11041 gtcgctttgg gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc gttcacgttg
    11101 gtcgcagcaa taaaacgttt caccggcaga ccgagtgact tcgccagcag acccgccgtc
    11161 aaatcgccga agtttccgct tggcaccgag acaaccagct ggttgcgcgc ctcctgcggc
    11221 agctgcgcaa cagcttcaaa gtagtagcaa atctgcgcca gcaaacggct gatgttaatc
    11281 gagttagctg agtttagccc cagcgccact ttcagttctt catcatcaaa cgcctgcttc
    11341 accagcgcct gacaggcatc gaaatcgccg tcgatggcaa cagtttcaat attgccgccc
    11401 aatgtacaga acagtttttc ttgcagtgga ctgattttgc ctcgtggata gaggataacc
    11461 actttcacat tcggcaaacc gtagaaagca tgagccactg ccgctccggt atcaccggag
    11521 gtcgcggtca gaatggtcac tggcttatcg cccgcaatat gggtcagcat ttgtgccata
    11581 aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt ggaacaattc cagacaaccg
    11641 acatcgcttt caacattggc gaccggagcc gggaagacaa acgccgcgcg tacgcgctct
    11701 tccaggattt cctgcgggat ttcatcacca ataaacgccg agaggatctt cgcactgcgg
    11761 gtgacaaaat ccagcttcag catctcatca atttcagtca ggctgaattc cggcaggtcg
    11821 tgcgggaaaa acagcccctg atttttgccc aacccctggg ttacggcttg cgcaaagctg
    11881 acctgctcat tgtgatcttt cagattgtag agtttcattt agttttccag tactcgtgcg
    11941 cccgccgtat ccagccggca aatatgaaca aaaccttcct gattttgcag gtagttctta
    12001 cccaaccagt cggcaacgcg ctgggcggta tccggcttgt cacacagagc gaacaaagtc
    12061 gggccggagc cggagatacc gctcgctacc gcgccgattt ccgcgaccgc ctgccgcgcc
    12121 tgccggaagc ctggcagtaa ccgttcacgg tagggttcag cgataacatc tttcatcagc
    12181 ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa taaagcctgc cagatgtcgc
    12241 ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta aaatagccct ggcttctgcc
    12301 gtcgagactt taatccccgg atacgccagc acccacagcc actcatcaaa ccctggcact
    12361 tgctggctga tgatgtcgtt ttcttcgatc atcaactgca taccaccgag aaaacacggt
    12421 gccacgttgt cgtaatgaat gctgccggag atacgccctt ccagctcgcc catcaaagcc
    12481 agcaaacggg tgtcattaag cggcttgccg cagtgttcat tcatcgccat cagcgccgcg
    12541 accactgagc aggcgctgga gcctaagccc gagccgatcg gcatattctt ttccagggtc
    12601 atcgccactg gaatttgctt acccagctcc tggcaaaaac gctcccaaca ctgataaacg
    12661 atattttccc gaggttctga cggcagctta tcggcaaagc gtccgaggtt attgagactg
    12721 aatgtctctg ccgcctcaac cgtgactaca tctccgagca atgcaccatc aacgggtgtc
    12781 accgccgccc cgagcacatc gaacccgacg ctcatattgg cactggaagc cggggcataa
    12841 actttaacca tgtcagactc ctaacttcca tgagagggta cgtagcagat cggcaaagac
    12901 accggcagct gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg gcagcggctg
    12961 ataatagtgg ctatagaagg ccagggcgtt ttcgccgttt ttcactttga acagcggatc
    13021 gttgccatcc acttcagcaa tcttcacgcg gcagacgcca tcttcatcaa tattgccaac
    13081 atagcgcaaa acttttcctt catcacgggc cttcgccaca cgagcggcaa agagatcatc
    13141 gagctgtgac agattcgcca taaaagcggc aacatcaccc tcggcgttaa actcagcggg
    13201 tagcacaggt tcaatttcaa tatccgccag ctccagttca cgtcccgttt cacgagcgag
    13261 aatcaatagt ttacgcgcca catccatacc agaaagatca tctcgcggat ccggttcggt
    13321 ataacccatt tcccgcgcca acgtggtcgc ctcggagaaa ctcatgcctt cgtctaactt
    13381 gccgaagata taagaaagcg aaccagaaag aatgccggag aacttcatca attcatcacc
    13441 tgcattgagc agattttgca ggttctcaat aaccggtaat ccagccccaa cgttggtgtc
    13501 atagaggaat ttacgccgcg atttttccgc cgcatgacgc aacagatggt agtaatccat
    13561 cgacgaggtg ttagcctttt tgttcggcgt gacgacgtgg aaaccttcgc gcaggaagtc
    13621 ggcatattga tccgccactg cctggctgga ggtgcagtca acaatgaccg ggttcagcag
    13681 atgatattct ttcacgaggc gaattaagcg cccgagatta aacggctctt tggcttgcgc
    13741 cagttcttcc tgccagtttt ccagattaag gccatgtaca ttggtgagca gagccttcga
    13801 gttggcaacg ccgcagacac gtaagtcgat atgtttattc ttcaaccagc tttgctgacg
    13861 cttcagttgc tccagcagcg caccgccaac gccaccgacg ccaatcacaa acacttcgat
    13921 aacctgatcg gtattgaaca gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc
    13981 gttatttacc acgacagaga ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt
    14041 gatattggcg cgggccagcg cggcaaagaa tttcgccgag atcccacgca aggtgcgcat
    14101 accatcacct accaccgaga taatggccag ccgttccgtc accgccagcg gctccagtaa
    14161 gccttctttc agttccaggt agaactcttc ctgcattgcc cgttcagctc gcacacagtc
    14221 gctttgtgga acgcagaaac tgatactgta ttcggaagat gattgcgtaa tcagcaccac
    14281 ggaaatacgg gcgcgtgaca tcgctgcaaa gacgcgcgcc gccatgccga ccatcccttt
    14341 catccccgga ccagaaacgc tgaacattgc catgttattc agattggaaa tgcccttgac
    14401 cggtaattcg tcttcatcac ggctggcacc aatgagcgta cctggtgctt gaggatttcc
    14461 ggtattttta atcaggcaag ggatctggaa ctgggcgatg ggggtaattg tgcgggggtg
    14521 aagaacttta gcgccgaagt aggaaagctc catcgcttcc tggtaggaca tcgacttcaa
    14581 caacctcgca tcgggcacct gacgcgggtc gcaggtatag accccgtcaa cgtccgtcca
    14641 aatctcgcaa caatcggcgc gtaaacaggc agccagcacc gccgcggagt agtcggaacc
    14701 gttacgtcca agcaccacca gttcgccttt ttcattaccg gcggtgaaac ctgccatcaa
    14761 caccatgtga tcagccggaa tacgacttgc cgcaatacgg cgggtggact cagcaatatc
    14821 gacggtagat tcgaggtaat gccccactgc cagcagtttt tcgaccggat cgataacggt
    14881 aacgttgtga ccacgcgctt ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc
    14941 acggcaaatc agcgcagcgt tgatgctatc cgggcactgc cccaacaaac taatgccatg
    15001 caggacatgt tttatttggg caaattcctg gtcgacgaaa gttttcagtt gcgccagcgg
    15061 gaatcccggc tgagcggcgg cgagtcccgt cagaagttcg gcaaaaatac gttcggcatc
    15121 gctgatattg ggtaaagcat cctggccgct aatggttttt tcaatcatcg ccaccaggtg
    15181 gttggtgatt ttggcggggg cagagaggac ggtggccacc tgcccctgcc tggcattgct
    15241 ttccagaata tcggcaaccc gcagaaaacg ttctgcattt gccactgatg taccgccgaa
    15301 cttcaacact cgcatggttg ttacctcgtt acctttggtc gaaaaaaaag cccgcactgt
    15361 caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta
    15421 atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta
    15481 atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa
    15541 taaaatttta atttactcac ggcaggtaac cagttcagaa gctgctatca gacactcttt
    15601 ttttaatcca caccgagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact
    15661 tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac
    15721 attgccgtgt ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc gaccagtttt
    15781 atgtcatccg ccactgccag agtcgtcagc agtgccatgg ctcgttcgcg taaagcttgc
    15841 agttgatgtt tgtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat
    15901 tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc
    15961 ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcaaccc ggaatcttcg
    16021 cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcagcacc
    16081 agttctactg gcgtagcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcaaca
    16141 gtgaaatcga catcgtgtaa cgattcagcc agtgtcggga aaactttaat attatcaata
    16201 atatcaccag atccatgtgc gacccagcgg gtggctggct ccaggtgtgc ctgactatcg
    16261 acaatccgca gctcgctaaa ccccatcgtt ttcattgccc gcgccgctgc cccaatattt
    16321 tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact cttcttgctc
    16381 aaataaccgc gaaccgggtg accactgtca acttattacg cggtgcgaat ttacaaattc
    16441 ttaacgtaag tcgcagaaaa agccctttac ttagcttaaa aaaagctaaa ctatttcctg
    16501 actgtactaa cggttgaatt gttaaaaaat gctacatatc cttatgttta cttaggataa
    16561 tttta
//
LOCUS       DAZGAP010000123        16517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGAP010000123 DAZGAP010000000
VERSION     DAZGAP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.124"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..701
                     /locus_tag="ACHLAM_003435"
     CDS             <1..701
                     /locus_tag="ACHLAM_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO7854907.1"
                     /translation="WFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFD
                     DAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKASASLAALWQVVN
                     QIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQ
                     LTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRG
                     MAKRIARLAEQSTD"
     gene            800..1813
                     /gene="ybjS"
                     /locus_tag="ACHLAM_003436"
     CDS             800..1813
                     /gene="ybjS"
                     /locus_tag="ACHLAM_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7854908.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1810..2640)
                     /gene="amiD"
                     /locus_tag="ACHLAM_003437"
     CDS             complement(1810..2640)
                     /gene="amiD"
                     /locus_tag="ACHLAM_003437"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO7854909.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKS
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2637..2960)
                     /gene="ybjQ"
                     /locus_tag="ACHLAM_003438"
     CDS             complement(2637..2960)
                     /gene="ybjQ"
                     /locus_tag="ACHLAM_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO7854910.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3086..3601
                     /gene="ybjP"
                     /locus_tag="ACHLAM_003439"
     CDS             3086..3601
                     /gene="ybjP"
                     /locus_tag="ACHLAM_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7854911.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3819..4547
                     /gene="artP"
                     /locus_tag="ACHLAM_003440"
     CDS             3819..4547
                     /gene="artP"
                     /locus_tag="ACHLAM_003440"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO7854912.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4565..5296
                     /gene="artI"
                     /locus_tag="ACHLAM_003441"
     CDS             4565..5296
                     /gene="artI"
                     /locus_tag="ACHLAM_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO7854913.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5303..6019
                     /gene="artQ"
                     /locus_tag="ACHLAM_003442"
     CDS             5303..6019
                     /gene="artQ"
                     /locus_tag="ACHLAM_003442"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO7854914.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTVLRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6019..6687
                     /gene="artM"
                     /locus_tag="ACHLAM_003443"
     CDS             6019..6687
                     /gene="artM"
                     /locus_tag="ACHLAM_003443"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO7854915.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6913..7644
                     /gene="artJ"
                     /locus_tag="ACHLAM_003444"
     CDS             6913..7644
                     /gene="artJ"
                     /locus_tag="ACHLAM_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO7854916.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7673..8800)
                     /gene="rlmC"
                     /locus_tag="ACHLAM_003445"
     CDS             complement(7673..8800)
                     /gene="rlmC"
                     /locus_tag="ACHLAM_003445"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO7854917.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLSVKHMWDLFCGVGGFGLHCAMPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYCIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(8841..9329)
                     /gene="ybjO"
                     /locus_tag="ACHLAM_003446"
     CDS             complement(8841..9329)
                     /gene="ybjO"
                     /locus_tag="ACHLAM_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO7854918.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9389..10366)
                     /locus_tag="ACHLAM_003447"
     CDS             complement(9389..10366)
                     /locus_tag="ACHLAM_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO7854919.1"
                     /translation="MSTLEPAAQPKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFS
                     SVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARRG"
     gene            complement(10376..11509)
                     /gene="potG"
                     /locus_tag="ACHLAM_003448"
     CDS             complement(10376..11509)
                     /gene="potG"
                     /locus_tag="ACHLAM_003448"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO7854920.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(11604..12716)
                     /gene="potF"
                     /locus_tag="ACHLAM_003449"
     CDS             complement(11604..12716)
                     /gene="potF"
                     /locus_tag="ACHLAM_003449"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO7854921.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(13067..13543)
                     /gene="ybjN"
                     /locus_tag="ACHLAM_003450"
     CDS             complement(13067..13543)
                     /gene="ybjN"
                     /locus_tag="ACHLAM_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO7854922.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(13631..14533)
                     /gene="rimK"
                     /locus_tag="ACHLAM_003451"
     CDS             complement(13631..14533)
                     /gene="rimK"
                     /locus_tag="ACHLAM_003451"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO7854923.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(14594..15316)
                     /gene="nfsA"
                     /locus_tag="ACHLAM_003452"
     CDS             complement(14594..15316)
                     /gene="nfsA"
                     /locus_tag="ACHLAM_003452"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO7854924.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(15300..15587)
                     /gene="ybjC"
                     /locus_tag="ACHLAM_003453"
     CDS             complement(15300..15587)
                     /gene="ybjC"
                     /locus_tag="ACHLAM_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO7854925.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            15747..16004
                     /gene="grxA"
                     /locus_tag="ACHLAM_003454"
     CDS             15747..16004
                     /gene="grxA"
                     /locus_tag="ACHLAM_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO7854926.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(16034..16411)
                     /gene="ybjM"
                     /locus_tag="ACHLAM_003455"
     CDS             complement(16034..16411)
                     /gene="ybjM"
                     /locus_tag="ACHLAM_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO7854927.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT         4116 a         4406 c         4115 g         3880 t
ORIGIN      
        1 tgtggtttct caatgtgatt acttccgtac cgcccaccac cgccagggcg ttgattcagg
       61 ggctgaaaca tgatctgctg gcggatgaca ccgcgctacg tgcactcatc ccacaacggc
      121 tgatcgcttt cgatgacgcg gtacgtcgca ccctgaaaga agaagaaaag ctggtcaact
      181 ccagcgactg gggctacgac gctcaggcct ttgcccgctg gcgaccggag tacggttatt
      241 tcgccaaaca ggcggggttt accgttaaag cgtccgccag ccttgcggct ttatggcagg
      301 tggtgaatca aatcggcggt aaagagcgtt atttctttgg caatattttg tggcagacac
      361 gggcgttgat ggaccgcgcg atcggtcata agctggcgaa aggccgcccg gagcgtgaat
      421 atttgcagac cggcgatgcg gtggatagct ggaaagtgat tgtcgttgaa ccggaaaaac
      481 aacttacgct gttatttggc atgaaagcgc cggggctggg acgactgtgt tttaccctgg
      541 aagataaagg tgactatcgt actatcgatg ttcgcgcatt ctggcatccg cacggtatgc
      601 cggggctgtt ttactggtta ttgatgatcc ccgcgcatct gtttattttt cgcggaatgg
      661 caaaacgaat cgccagactg gcagaacaaa gcacagatta atcgacaaaa cctgcgttat
      721 tctttcatgt ttcccattgc atcatggggg aaatcacgga agaatgcgca cgttattttc
      781 tcaacaacag tggattgata tgaaggtact ggttaccggc gccaccagcg gcttaggtcg
      841 aaacgcggta gagtttttat gccagaaagg catcagcgtg cgagcgaccg gtcgcaacga
      901 ggcaatgggt aaattgctgg aaaaaatggg cgcagagttt gttccggcgg atctgaccga
      961 gctggtctca tcacaagcta aagtgatgct cgcgggcatt gatacgctgt ggcactgctc
     1021 cagctttacc tcgccctggg ggacacaaca ggctttcgat ctggctaacg ttcgcgccac
     1081 tcgccgcctg ggtgaatggg ctgtcgcctg gggtgtgcgt aactttattc atatctcttc
     1141 accctccctg tacttcgatt atcaccacca tcgcgatatt aaagaagatt ttcgccctca
     1201 ccgcttcgcc aacgagtttg cccgtagcaa agcagccagc gaagaggtga tcaatatgct
     1261 ttcgcaggcg aatccacaaa cgcgctttac tattctgcgc ccacaaagtc tgttcggacc
     1321 gcacgataaa gtctttattc cccgtctggc gcatatgatg caccactacg gcagcattct
     1381 gttaccgcat ggcggcagtg cgctggtcga tatgacctac tatgaaaatg ccgtgcacgc
     1441 catgtggctg gcaagccagg aagcctgcga taagctacct tccgggcgtg tgtacaacat
     1501 caccaacggc gagcatcgca cactgcgcag catcgtgcag aagctgatcg acgagttgaa
     1561 tattgactgt cgtattcgtt ccgtccccta cccgatgctg gatatgatcg cccgcagcat
     1621 ggagcgttta ggccgcaagt cagcaaaaga gccgccgctg acccactacg gcgtctccaa
     1681 gttgaatttt gactttacgc tggatattac gcgcgcgcag gaagagttag gttatcagcc
     1741 ggtcatcacc ctggatgaag gtatcgagaa aactgccgcc tggctacgcg accacggaaa
     1801 actgccgcgc taatcctgcc cgtatttctc cagcaacgct tcggcaatcg cctgagtttc
     1861 tgcatccgct tcgccgttgt ataacgtcgg gcggaaatgc atctggaacg ccataatcac
     1921 gcgccgctgc tcgcgcggtg tcatatcagg tttaacgtca taaccgtagc gcgccaaaag
     1981 ctccagtaat gatgcggtat ctaccagtgt gtgcggcgcg cgcccggcaa ggtaaaagtt
     2041 cacccgctgc gcatccggcc aggcaccaat cccctgctgc gccagttgtt gccaggggaa
     2101 taacggcccc ggatcgtctt tgcgctgcgg cgcaatatcc gcatgcgcca ctacgttttc
     2161 cgacttgatg tgataacggg caataatatc tttcgccagc ggaataagcg cctgaatctg
     2221 tgcctgttca aacggggcaa aatatttcac tccggatgat ttttgccagc cacggttttc
     2281 cagctcaatg ccaatagagg tgtcgttaag acgcgttgcc ccgcgccagg cgctaatccc
     2341 cgcatgccag gccagttcct gttccggcac cagttgccag atgcgcggtt taccgttgta
     2401 tcgcggtggt accgcaggga ccagataatg cgagctgacc tgcttatcgg tcagtgtcgc
     2461 cagcgagcta tcaaaatcat ctgcggtgta gtggataacc agcactttaa tgcgcggata
     2521 cgccgcctgc gcctggcgtc gggtatcaag ctgatatccc tctttctcga caatgccttt
     2581 ttcgcctgca caccctgcca gtaacagagc gacagcgacc agccaaaaga ctcttctcat
     2641 cgacgcgttt tcaccgccgt accgctgacg ctaaccatca gcatactgcc attttgcccg
     2701 accgtttcgt agtcgatatc aataccgact acggcatcag cccccagcgc ccgggcctga
     2761 tcgccgagct cctcaaaggc gatctcccgc gctttacgca attctttttc ataagccccg
     2821 gagcgtccgc caacgatatc gcggataccg gcaaagaaat cacggaaaat attggcacct
     2881 aaaatcgctt cgccggtcac cacaccgcaa tattcgacga tggtctggcc ttccagagtt
     2941 ggggttgtag aaaattgcat gatcgatctc cttgtttgct aagccttcga tcttcaaagc
     3001 attatcggac tgatacgcta ttattgaaag gatatcatta ttatcgtaga acgctttcaa
     3061 ggtgttcgct tataaggaaa tcatcatgcg ctatagcaaa ttgaccatgc ttatcccttg
     3121 cgcactgcta ctcagtgcct gtaccaccgt cacgccagct tataaagata acggcccccg
     3181 caccggttcc tgtgtccagg gggggcctga tagcgtggct caacagttct atgactaccg
     3241 cattcaacat cgcggcaatg acattaccgc tctgcgcccc tatttgagtg acaaactggc
     3301 aaccctactt agcgatgcca gccgcgataa tagtcatcgt gaattgttaa gcagcgatcc
     3361 gttctccagt cgaaccacct tgccagacag cgcccacgtt gccagtgcat cgactatccc
     3421 taatcgtgat gcccgtaaca ttccattgcg cgtggatctc aagcagggtg accagggctg
     3481 gcaggatgaa gtgttaatga ttcaggaagg ccagtgttgg gtcatcgacg atgtgcgcta
     3541 cttaggcggt agcgttcatg ccacagcggg tacgctccgt cagtccatcg agaatcgcta
     3601 acgtcttgat caaccaataa accccgtaaa ttgtacggca tttcgtgagg aatgccgaca
     3661 tttatgctcg ccgaccaccg cccccgttat tttgtgctat gtttattgaa taatgcgctt
     3721 tgcttttaac ttttaaagca gaaatattgc ataattattc tgtcaaaggt actatcttcg
     3781 acctcaatag ctatcagact gccagtatac gagtgtcaat gagtattcaa ttaaacggca
     3841 ttaattgctt ctacggcgcg catcaggcgc tgttcgatat cacgctggat tgcccacagg
     3901 gcgaaacgct ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg ctgcgtgtac
     3961 tcaatctgct tgagatgccg cgctccggta cgctcaacat tgcaggcaac catttcgatt
     4021 tcaccaaaac cccctctgac aaagcgattc gcgatttgcg tcgtaacgtt ggcatggtgt
     4081 ttcagcaata caacctgtgg ccgcatctga ccgtgcagca aaacctgatt gaagcgccct
     4141 gtcgtgtact ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa ctgctggaac
     4201 gtctgcgtct caaaccttat agcgatcgtt acccgctgca tctttctgga ggtcagcagc
     4261 agcgtgttgc tattgcccgt gcgttgatga tggaaccgca ggtactgctg ttcgatgaac
     4321 cgaccgccgc actggacccc gaaattacgg cacaaatcgt cagcatcatt cgtgagctgg
     4381 cagaaacgaa tattacccag gtgatcgtca cccacgaagt tgaagtggcg cgtaaaaccg
     4441 ccagccgagt ggtgtatatg gaaaacggtc atatcgtaga acaaggcgac gcgagctgct
     4501 ttaccgagcc gcaaaccgaa gcatttaaaa actatctctc tcactaagat tcgggataac
     4561 aacaatgaaa aaagttctga ttgccgcgtt aattgcaggt tttagtcttt ccgccacagc
     4621 agccgaaacc attcgttttg ccaccgaagc ctcctatcct ccgtttgaat cgattgatgc
     4681 aaacaaccag atcgttggtt ttgatgtcga cctggcgcag gcattgtgta aagagattga
     4741 tgcaacctgc actttctcta accaggcgtt tgacagcctg atcccaagcc tgaaattccg
     4801 tcgcgtagaa gcagtgatgg cgggcatgga tatcactccg gagcgtgaaa agcaggtgct
     4861 gtttaccacc ccgtactatg acaactctgc cctgtttgtg ggtcagcaag gcaaatacac
     4921 cagtgttgat cagctgaaag gcaaaaaagt cggcgttcag aacgggacga cacaccagaa
     4981 gttcattatg gataagcatc cggaaatcac taccgtgcca tatgacagtt accagaacgc
     5041 aaaactggat ctgcaaaacg gtcgtatcga cagcgtattt ggtgacaccg cagtggtaac
     5101 cgaatggctg aaagataacc cgaaactggc ggcagtgggc gataaagtga ccgataaaga
     5161 ttacttcggt actggcctcg gcatcgcagt acgccagggc aacactgaac tgcagcagaa
     5221 actcaacact gcgctggaaa aagtgaagaa agatggcact tacgaaacca tctacaacaa
     5281 atggttccag aagtaattcc tgatgaatga attttttcct ttagcaagcg ccgccgggat
     5341 gaccgtcggc cttgccgttt gtgcattgat tgtcgggctg gcgctggcga tgttctttgc
     5401 ggtatgggag tctgcaaaat ggcgtcctgt cgcgtgggca ggttcagcgt tggtgacggt
     5461 tctgcgcggc ctgccagaaa ttctggtggt gctgtttatc tattttggct cctcgcagct
     5521 gctgctgacg ctttcggatg gcttcactat caatcttggg ttcgtgcaga tcccggtgca
     5581 gatggacatt gagaacttcg acgttagccc gttcctttgt ggtgtcatcg ctctgtcact
     5641 gctgtatgcc gcctatgcct cgcaaacgct gcgcggcgcg ttgaaagcgg tgccggtggg
     5701 ccagtgggaa tctggtcagg cgctggggct gtcgaaatcg gctatctttt tccgtctggt
     5761 gatgccgcag atgtggcgtc atgcgctgcc gggtctcggt aaccagtggc tggtgctgct
     5821 gaaagatacc gcgctggtca gtttgattag tgtgaatgat ttaatgctac aaaccaaaag
     5881 catcgctacc cgtacccagg aaccgtttac ctggtacatt gtggcggcgg cgatttacct
     5941 ggtgatcacc ctgctcagtc agtacattct caaacgcatt gacctgcgcg cgacacgttt
     6001 tgagcggagg cccagctaat gtttgagtat ttacccgaac tgatgaaagg gttgcacacc
     6061 agcctgacgc taaccgttgc ctcgctgatt gtggcactga ttctggcatt gatttttacc
     6121 atcatcctga cgctgaaaac gccggtgctg gtgtggctgg tgcggggcta tatcacgctg
     6181 tttaccggta cgccgctgct ggtgcagatc ttcctgattt attacggacc gggccagttc
     6241 ccgacgttgc aggagtatcc ggcactgtgg catttgttgt cagaaccgtg gctgtgtgcg
     6301 ctgattgcgt tgtcgctgaa cagtgcggcg tataccacgc agctgtttta cggtgcaatt
     6361 cgtgcgatcc cggaaggtca gtggcagtcc tgtagcgccc tgggaatgag caaaaaagat
     6421 acgctggcga tcctgctgcc gtatgccttt aaacgctcgc tctcttctta ttccaacgaa
     6481 gtagtgctgg tgttcaaaag tacctcgctg gcctacacca ttacgctgat ggaagtgatg
     6541 ggatacagcc agttgttgta cggacgcacc tacgatgtca tggtgttcgg cgcggcaggg
     6601 attatttatc tggtcgttaa cggcctgctg acgctgatga tgcgtctgat cgagcgtaaa
     6661 gcgctggcgt ttgagcgaag gaattaattt ggctgcgtgc tgatgccgga tgcggcgcgg
     6721 acgccttatc cggcctacat agtgcataaa ttcttataat gaagacggac aactcactaa
     6781 gttgtccgtc tttttttatt tcatttaaat tatttaatca tatttattgc atataaattc
     6841 acttgatggc attgttatcc catgccgcag acacggccaa aaatcataag attgacagac
     6901 gggagttcca tcatgaaaaa gttagttctt gccgcattac ttgcttcctt tactttcggt
     6961 gcttctgccg cagagaaaat caattttggc gtttcagcca cctatccacc ctttgaatct
     7021 ataggtgcta ataatgagat tgtcggcttt gatatcgacc tggcaaaagc cttgtgcaaa
     7081 caaatgcagg cagaatgtac ttttactaat cacgcgttcg acagcctgat cccgtcactg
     7141 aaattcagaa aatatgacgc cgtaatctcc ggtatggata tcactccaga gcgtagcaaa
     7201 caggtatcgt ttaccacgcc ctactatgaa aactcagccg tcgtgattgc caaaaaagat
     7261 acctacaaaa cgtttgccga tctgaaaggc aaacgtattg ggatggaaaa cggtactacg
     7321 caccagaaat atattcagga tcagcacccg gaagtgaaaa ctgtctccta tgacagttat
     7381 cagaatgcct ttatcgatct gaaaaatggc cgtattgatg gggtatttgg tgacacagcg
     7441 gtggttaatg aatggctgaa aaccaacccg caactgggcg ttgcgactga aaaagtgacc
     7501 gatccgcaat acttcggcac cggcctgggt atcgctgtac gtccggataa caaagctctg
     7561 ctggaaaaac tgaataacgc gctggcagca attaaagctg acggtacata tcagaaaatc
     7621 aatgaccagt ggttcccgca gtaagattca gaaagccgca cctgcggctt ttttattgct
     7681 taaccagcag tgtcagcact tcgtaatgcg cagtgtgcgg gaacatatca aaaagctgta
     7741 cccgttcgat gcagtaacct ggcagttcgc ggatatcttt cgccatggtt tgggcgttac
     7801 agctggagta gataataaaa cgcggtgcca tcgttgagag ataatcacac agcggtttac
     7861 caatgccgcg gcgcggcggg ttaaccagca ccagctccgg cacctccccc tgagcagtgg
     7921 caaactgagt ggagtccagc gcctgaaatt gcaaacgcgt taagcccagt tcagcggctg
     7981 actgctttgc acaggcaatg gcctctggcg caatttcgat cccggttaac tgcatgtcag
     8041 gcatcgcgca gtgtaaacca aagcccccca caccgcagaa cagatcccac atatgtttaa
     8101 ccgacagctg tcgtacccag tcgcgcgcgg tggcgtacaa ctggctggcg accgccggat
     8161 tagtctggaa gaaactttgc ggacggatcc acagcggtac gtcattaaaa cgctctgcca
     8221 gcgcctgttg ttcagtcagg taaatctccg tctccccttc cataatcgcc atatgtaccg
     8281 gctgaatatt gacggtaata actttcagct gcggtagttg ttcctgtaac cacggcagcg
     8341 ccttacgcag ttgcgccagc ttggtatcag aacgcagtac aaagcgcagc atcatgccgc
     8401 catcgctctg gctttcagtc agcagaatgt atttcagttc gccacgctta cgcgccacgt
     8461 tgtagggcgt taaccccgct cgggcgataa acggttttag cgccgcaaaa acgggcgcaa
     8521 atgaggcagg ataaagcgga cagtcacaaa ggtcttccgg cgtgccgtcg cgatgcagca
     8581 taccgagcag tggtttttca acgctaccac tcaccaccat tttggcttta ttacgaaacc
     8641 cttgttccgg gcctgacacc ggcgcgcacc attcctcaac cggaaagtcg gcgagcagat
     8701 ttttaagatc ggcggtttta gcggagagtt gctctggaat cggctgcgtt atccactgac
     8761 aggaacgaca gcgacccgcg tcgtaaagtg cgcactgcat acattgacct tcacatcatc
     8821 aggggcgacg attatacaca ttattgcaac tggaagaacc gccgactggt cgagggaacg
     8881 aacagcagca tcaggatgag catatccggc agcttctgca gcatcaggct atggaagatt
     8941 tcacgttttg attcaccggg aatgctgaac agctccggat aaccataccc cagcgaagct
     9001 gcccacaagt aacttgcggc cgtgatttgt gtcaacagat acagccagcg cgcccagcga
     9061 cgccctttca ccagtgaaaa cgcacaccag atctcaatga aaaccagcac cagactgctc
     9121 aaaaagacca gcgttaaact ccaggtttgt acgctgcgat gaatgaactc gccaataccg
     9181 cgcacgccca gcgtattgaa aatcatcagc acgtcgaggc cacggatcat aataatggcg
     9241 agcgccgcca cctgcaccag cgcaggcaca ttcaggcgag catgagatga agatgttttc
     9301 ttaaaaaatc ccaacgtttc gtcttccatg aaaacaatgc cgcgacatgc gcggcattat
     9361 gtagccaggt tggcaaattt tagtgtcttc agccacgtct tgcacgctgg atgtcgcgta
     9421 tccgctgttt ttctgcgcgc gccatcagat accaggcgat aaatccgaca attccgaccg
     9481 cgccgaggat caatgttgcc agggcgttga tttccggatt cacccccatc cgcacgctgg
     9541 aaaagaccag catcggtaaa gtggtggctc ccggcccgga aacaaagctg gcgatcacca
     9601 gatcatcaag cgacaaagta aaagccagta accagccaga aatgatcgcg ggcatgatca
     9661 tcggtagcgt aatgacaaaa aacactttca gcggcgtcgc accgagatcc agtgccgctt
     9721 ccaccagcga ataatcaata cgaatcaagg cggtgtagat cggcagcacc ataaacggca
     9781 cataggcgta aacaatgccg atataaaccg ccaggttggt atgcagaatg gtcaacggtt
     9841 gatcgataac ccccagccac agcagaaaat tattcagcac accgttgttt tttaatattc
     9901 ccatccaggc ataaacgcgg atcagaaacg aggtccacga cggcaggatc accagtagta
     9961 ataagatatt acgggtcgaa ggcttactgt gcgccaccgc ccacgccagc ggatagccga
    10021 tcagtaaaca gcaaaatgtc gaaatcgcag ccacctgtag cgactggaga taagcatcga
    10081 aatagagcgg atcgtcagtc agttgcagaa aattaccgag attaagagtg atggacagtt
    10141 gcccgtcagc ccactccatc agttcggtat aaggcggaat ggcgcgcgcc atctccgcca
    10201 gacttatttt aaagacgatc agaaatggca gcagaaacag caggatcaac cagatatacg
    10261 gcaacgcaat gaccagtttg cgcccatgct tcatttgcag ctgcgacaac cacagcttaa
    10321 atccgcccgg cggtttgggc tgggcagcag gttcaagtgt actcatcttt gctccttaaa
    10381 ccgtcagcac cacacagctg tccacttccc agcacaaacg cacttcgtcg ccccaggttg
    10441 gtaacccttt gcgatatcga tgagcgtttt gtagctgagc gctgatcatc tgtccgctct
    10501 tgagacgaac gtgatacacc gaaagatcgc cgagataggc aatgtgcatc acctcaccta
    10561 ccgcaaagtt aaaaccgtca gcgggaggtt cctcgcaaag catgattttt tccgggcgca
    10621 gcgccacatg caccggcaca ttatcgacca ccgaagcatc tacatcgact ttcagtggat
    10681 gcaccagccc cggcgaataa agcaccagac catcttcctg acgctctttg agtacacctt
    10741 caaagacatt aaccgagcca ataaattcgg cgctgtagcg agtggtcgga tgctcgtaga
    10801 tctcttccgg ttcgccaatc tggacaaatt tccctcgatt cataatggcg atacgccccg
    10861 ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac acaagtcaca ccgacacgct
    10921 ccagaatatc caccacttca agctgcatcc ggtcacgcag ctttttatcc agcgcgccca
    10981 taggctcatc gagcagtaat agtttcgggc gcttggcaag gcttcgggcc agtgccacac
    11041 gctgtcgctg accgccggaa agctgatgcg gtttgcgttt ggcgaactcc tgcatatgca
    11101 ccagcccgag catctcattg acccggctgg caatttccgc tttcggtagt ttgtcctgtt
    11161 tcaggccaaa agcgatgttc tgttccacgg tcatatgcgg aaacagcgcg taagactgaa
    11221 acatcatatt tatggggcgc aggtaaggcg gaacctgtga caaatcgacg ccatcaagca
    11281 ttatctgtcc ggcagaaggt tgttcgaaac ctgccagcat acgcagcagc gtggacttgc
    11341 cacagccgga tgcgcccagc agcgcgaaga tttcgccttt atagatggtc aggctgacat
    11401 catcgaccgc atgctgacca tcgtaagatt tagtcagatt acggatttct aatagcggcg
    11461 tcagcacctt acgggttttc gcctgcgggc gagggatagc gtcattcacg gctgctctcc
    11521 ggcattgaat gtgtgcaagc gcaccataat ggtgcgaatt gccggcgggc gtggtgcgcc
    11581 cgtccggcat ctacgactgc ggattatttt ccgctcttca ctttggtcca cgcgcgggtg
    11641 cgcacacggt cgattttcgg atcctgtact ttcagagtga acagcttcgc acgaacatcc
    11701 gcaggcgggt aaatacccgg gttatcacgg acttccgcac tcaccagcgg cgtagctgct
    11761 ttattggcgt tagcatagaa cacatggtcg gaaatatgcg ccaccacatc cgggcgcagc
    11821 aggtaattca ggaactgata ggcttcgtct ttatttttgg catccgcagg catggcgaag
    11881 acatcaaaga acgccatcgc cccttctttc ggaatcgaga acgagacatt aacgccattc
    11941 ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcgc ccgcccagcc aatagcgacg
    12001 cagatatcgc cgtttgccag gtcgttaatg tattgagatg aatggaaata acgaatgttc
    12061 gggcgcagct ttaacagcag atcggttgcc ggtccggtgt aatcatctgc tttagtgctg
    12121 ttgggatctt taccgagata attcagcacg gtagcaaaca cttcttccgg tgcatcaagg
    12181 aaagagacgc cgcagctttt cagtttttcc agattttcag gcttgaggat caagtcccag
    12241 ctatcgacgg gcgcgttttc gcccagcacc gctttaactt tatcaacgtt atagccaatc
    12301 ccggtcgtcg cccacatata gggcatagca aatttattgt cgggatcgtg tttggcaact
    12361 aacttcagca gctccggatc gagattcttc cactccggca atttgctttt gtccagcggc
    12421 tggaaaactc ccgcagtcaa ctggcgttca agaaagcttg ctgacggcac caccaggtca
    12481 aagccggtgc tacccgccat taatttgcct tccagtactt cgttagagtc gaaaacatcg
    12541 tagacgactt taataccggt ttctttttca aaattggcca ccgtgtccgg ggcgatataa
    12601 tcagaccagt tataaatgtg aagtgttttt tgttcagccg cgagcgtgcc gacagagacg
    12661 gccatcagag cacccgtaac cagacccgat agccattttt tatttaaggc ggtcatatct
    12721 ctttccttct gaaagttcgt taacaaacga acaaaagttg tcacactgaa agatatgcaa
    12781 taaacgtgca tattttttat cggcgcggtg taagaaaaga gaactctctc ccggccagca
    12841 ctgctcgcta tataaaagca tcgcaagaat aactttagcc agggtttgag actatagctc
    12901 ggattaaaaa acggtattta acaaattttt atgcgtattt gctctatgtg ataaagccaa
    12961 atcggcacaa gagaagtgaa ataatcttta agaaaaggtt aagaatagca gataattttg
    13021 cagcacgcag tcactgcggc agcgactgcg tactcgaaag gagttatcag tggaggaaat
    13081 agttttcggt aacgttgttt tgcccttcat cgtcagtcgg taacagcagt tgatgggcat
    13141 tagcttcaag aatgaccatc gaaatctgct cttcgctctg acgcacgaac catgcaaact
    13201 gctcataggt tacgccctgc ataacggata aagactggca aaccaccagc tttggcagat
    13261 tatcatcctg catatcaaga aatgctttca cggtcagcga actggcattg atggcagata
    13321 aatccgccgc cagcggcaat acggctgaag gtcggacttc cgccatggca gaaaacagga
    13381 tcgtgttatc gatcagatcg attttggcat caaagacacc gtcgaaattc tgcatatggg
    13441 gcagatgcag agcctgacag ttatcacatt caaaaaaact catccccagg tcatcgagcc
    13501 attgacgcag cgtatccaga ccaggaacga ccagcgatgt cataattttg tacaacctct
    13561 ttcgataaaa agaccggcac agcttacgca aaaagcgcag gcaaaaccat gatcagtaat
    13621 gtgattgcga ctaaccaccc gttttcaggc aatattccgt cgtagcgtgg cgttcgatcc
    13681 agcggatcat tttacccgcg atgtcgatac cggtggtttt ttctattcct tccagccccg
    13741 gcgacgcatt cacctccatc accaatggcc cgcgattagc acgcagaata tccacaccag
    13801 caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat ttcacgctcc tgtggtgtga
    13861 tactggcgac gcttgccgcg ccgccacgat gcaaattgga acgaaaatcg ccctctttcg
    13921 cccgccgttc aatcgcagcg accacttcat cgccaacaac cagacagcgg atatcgcacc
    13981 cttgcgcctc tttgatatat tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt
    14041 caatcacgct ttccgccgcc tggcgcgtct ccgccagcac gacaccaatt ccctgcgtgc
    14101 cttcaaccag cttgaccacc agcggcgcac caccgaccat gtcgattaaa tcgctggtat
    14161 catccggcga atgcgcaatg cccgtgacag gcaggtcgat gccctgacgc gccagcagtt
    14221 gcatggaacg caatttgtca cgcgcccggg caatggcgac cgactcattg agcggatagc
    14281 tccccagcat ctcgaactgg cgcagtgccg ccgtcccata aaaggtaatg gcggtaccaa
    14341 tacgcgggat cacagcatca aaatggggta acttgcggcc tttgtagtga atagaagacg
    14401 ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc
    14461 gctgtatagc ggcttcacgc agccgcttac acgaatagag cgttccatcc cgggacaata
    14521 tggcaatttt caccctgcac ctctctgtca gacctggtca aaagcccgcg tatcatacat
    14581 cggcgtggcg gttttagcgc gtcgcccaac cctgtttgtg caaataatcc agtataaatg
    14641 ggcggctttc tttaatgatt gttcggcgga tatgatcgct ccaggtatcc cggcgattat
    14701 tgctgccacg ggtgaggtaa tattccgcca gttgttcgtc atactgcgcc agtgcgtctt
    14761 tatccagcgg ttgatagttg ttttcatgca ccaaaatgga ggccggtaaa cgcggcttaa
    14821 gatccggatt atccgcaggc cagccaaggc acagcccaaa cagcggcaga acatgctgcg
    14881 gtaatttcag cagctccgtc accgcttcaa tattattgcg caggccgccg atatataccc
    14941 cgcccaatcc cagcgattcc gctgcggtta atgcattctg cgccatcatt gccgtatcaa
    15001 cgacaccgag caacagttgt tccgccaggc caagttgtgc atccggacag atctgtaaat
    15061 ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg tgtttttgcc
    15121 cgccggtcag cgtcaccagt tcatcacgta acgctttgtc ggtaatgcga ataatgctac
    15181 tgcactgcaa aaaactggaa ctggacgtcg cacgggcgct gttaataatc gcctcacgct
    15241 gcgcttcgga aatgggttca tcagtgaaat ggcgaatgga gcgatggcca caaataagtt
    15301 caatggttgg cgtcattatc tttttctctt tctgaacgtg aatattgcgg tggacggttc
    15361 atcagctgtg gggcaagacg ttttgccacc tgaagaataa ccaccaccgc agcgggaagc
    15421 atgagcaaaa caccgagaaa aatcatcaga atctgcactt ctggccgaga aaatggctca
    15481 ggcagcgaca gggagccgct taccgacagc agcgccaccg ccagcaacat cattccgata
    15541 aattccagta tcaacacgct tttaggcaat ttaccgatcg cgcgcatacg cttccctctg
    15601 caaagtgagc cttcagtcta aaacttttca ctgtattgtg tttaacagtt atagctttta
    15661 gcaattaatg caacaggtta aacctacttt cagcgaatac attttagcgt gatcattaca
    15721 ggtataaatc tatgaggaga gaaataatgc aaaccgttat ttttggtcgt ccgggttgcc
    15781 cttactgtgt gcgtgcaaaa gatctggctg agaaattgag caatgaacgc gatgatttcc
    15841 agtatcagta tgtagatatt cgtgcggaag ggatcactaa agaagatcta caacaaaagg
    15901 caggaaaacc cgtagaaacc gtgccgcaga tttttgtcga tcagcaacat atcggcggct
    15961 ataccgattt tgctgcatgg gtgaaagaga atctggacgc ctgatcgtct gacaagccct
    16021 cgcgttgagg gctttattga ttttttctgt gctgtggttt aaacaaacta ctgataaata
    16081 agaaacacaa tgcccccagc gcacaccaga acaccgcact taataaccat gccagctctt
    16141 gccagaatga gcgcgtcggt gaaaaaaaca gccgcataat gagcatcgaa cagggtgccg
    16201 ccagcattgc gccaaacaga ggtttcagga cttctctacg atgtgaaaag aagctggcaa
    16261 ctgcaccagg aagaatgaaa aatagcaaac cgatttcagg atgcccggca gcccgaaaag
    16321 cgcctttcat gtgcgtcgcc agaaaaagac acaccacaat gaagaggaca aaacagcaga
    16381 ttgcccccgc ccaacgttgt ttatgtttca ctcgttcctc ctgacactgc gtctatcgaa
    16441 cacatttttc gccagtgtgg cgttcagtaa gataaagccg cttcgcattc catgctaata
    16501 taggccaacg caattca
//
LOCUS       DAZGAP010000124        16248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGAP010000124 DAZGAP010000000
VERSION     DAZGAP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.125"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..169)
                     /locus_tag="ACHLAM_003456"
     CDS             complement(<1..169)
                     /locus_tag="ACHLAM_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO7854928.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLP"
     gene            complement(326..661)
                     /locus_tag="ACHLAM_003457"
     CDS             complement(326..661)
                     /locus_tag="ACHLAM_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854929.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESFEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
     gene            complement(778..1020)
                     /locus_tag="ACHLAM_003458"
     CDS             complement(778..1020)
                     /locus_tag="ACHLAM_003458"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO7854930.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(1101..2795)
                     /locus_tag="ACHLAM_003459"
     CDS             complement(1101..2795)
                     /locus_tag="ACHLAM_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7854931.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIEGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            complement(2822..3442)
                     /locus_tag="ACHLAM_003460"
     CDS             complement(2822..3442)
                     /locus_tag="ACHLAM_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7854932.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAVFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(3708..4373)
                     /locus_tag="ACHLAM_003461"
     CDS             complement(3708..4373)
                     /locus_tag="ACHLAM_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO7854933.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(4514..5155)
                     /locus_tag="ACHLAM_003462"
     CDS             complement(4514..5155)
                     /locus_tag="ACHLAM_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO7854934.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            5858..6730
                     /gene="repA"
                     /locus_tag="ACHLAM_003463"
     CDS             5858..6730
                     /gene="repA"
                     /locus_tag="ACHLAM_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7854935.1"
                     /translation="MAALNQTSSTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAVYVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALF
                     AALDISEEAVASARRSRVEWENRQRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            7637..7906
                     /gene="yacA"
                     /locus_tag="ACHLAM_003464"
     CDS             7637..7906
                     /gene="yacA"
                     /locus_tag="ACHLAM_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO7854936.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDKMSRKVAGK"
     gene            7906..8184
                     /gene="yacB"
                     /locus_tag="ACHLAM_003465"
     CDS             7906..8184
                     /gene="yacB"
                     /locus_tag="ACHLAM_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO7854937.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            8230..9078
                     /locus_tag="ACHLAM_003466"
     CDS             8230..9078
                     /locus_tag="ACHLAM_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7854938.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGGRPVIIFNARFDIRILKQTAAAHSDPAYWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLASAASQAGLTWEGLAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            9219..9677
                     /locus_tag="ACHLAM_003467"
     CDS             9219..9677
                     /locus_tag="ACHLAM_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7854939.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQRASRLAASV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
     gene            complement(10041..10466)
                     /gene="nudI"
                     /locus_tag="ACHLAM_003468"
     CDS             complement(10041..10466)
                     /gene="nudI"
                     /locus_tag="ACHLAM_003468"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO7854940.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(10468..11175)
                     /locus_tag="ACHLAM_003469"
     CDS             complement(10468..11175)
                     /locus_tag="ACHLAM_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFO7854941.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYDAYMVLWSRINELLTEPDIIAFINYPIAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            complement(11172..12038)
                     /locus_tag="ACHLAM_003470"
     CDS             complement(11172..12038)
                     /locus_tag="ACHLAM_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO7854942.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKDKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            12523..12774
                     /locus_tag="ACHLAM_003471"
     CDS             12523..12774
                     /locus_tag="ACHLAM_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7854943.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            12771..13058
                     /locus_tag="ACHLAM_003472"
     CDS             12771..13058
                     /locus_tag="ACHLAM_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7854944.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            13226..13630
                     /locus_tag="ACHLAM_003473"
     CDS             13226..13630
                     /locus_tag="ACHLAM_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFO7854945.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            13939..15423
                     /locus_tag="ACHLAM_003474"
     CDS             13939..15423
                     /locus_tag="ACHLAM_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7854946.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(15644..>16248)
                     /locus_tag="ACHLAM_003475"
     CDS             complement(15644..>16248)
                     /locus_tag="ACHLAM_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7854947.1"
                     /translation="KDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATESVS
                     SGISSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEGGG
                     FLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNLDG
                     QPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
BASE COUNT         4225 a         3743 c         4011 g         4269 t
ORIGIN      
        1 gcggaagcag acgtttttgc gtatgggcga taatggcagc gctttcgcga atttgtccgt
       61 cgacgaacac cagagccgga ggtgtggtgt caggaacagg cgtggcatta aatgccgtac
      121 agccgctgag taatgacggc agaaggagtg taagtgattt tttttgcata gaataaaatc
      181 cggtatatta cgggttacgg tataagacag gtggcgggta actggccttc agatgaatat
      241 catttcatcg ggttattgat aatgaacgtt catgttcgtg gggggattat aagagtgaat
      301 tgtattttag aacgggagca ttattttatt tcttgcctgt tattgaaaca tttccggtta
      361 tgatctccga atggcaggta tcaataaaat cacttttccc cgattgtgtg tcaaagtcaa
      421 cgtgggtgaa cctgtgtaca tcctgccata tggcgaaaca gacaggacag gtaacaggta
      481 gtttgccttc agctattttt tcgaagcttt caaccagttg ctgcgccttg ttgtatctga
      541 cgttatagcc atttgtgcaa agcgtttcat acccatgagc tcctgtaact atatgtgtgg
      601 cgattctttc gtggactctc ccattaatta ataattgttc acgcgttgtg attgttctca
      661 tgcctgtttc tctgttggtc ataacataaa ataatctgca ttcctgaaaa atatcgcggt
      721 tgtgaaatat ttaattttta tccccctttt atgggggata aaaagcaggc tgtcaggcta
      781 taactgaacg cggtttctgg attttggtgc taatgtcaca tctttccatt cacagctgct
      841 tccgggtata cgctctttgc gtcgtattat acgggagttc tgacacacaa taattaagcc
      901 attgtctgcc gtctgaaatg gcattatagc gatgtttata tcttctgttg caggaatata
      961 actgactttc ttttcacatg tattggtgta tatgacaact ctgaattttt ctgcggacat
     1021 tttcaatcct cttttaaacc agaatatcct gtgagggcat tctttgtgaa aaagatgccg
     1081 gaaaaattcc ggcacgggga ttaaacctgt tgtggttctg agttatccgg agcggttacc
     1141 ggttcgctga catccggaac cttttccggg gctggcatga ccagtggacg tgtcagggct
     1201 gtaagtcctt caccggataa ccccacctcc ttcagcaggg agtcgatgag cggagcctgt
     1261 gtacggtaag ccagagcggc ggttaccgcc tgttcagcga gatttccgcc acttatgtcg
     1321 gttgttgctg ttttaccacg ggtaacaccg ccattcaggc catccacctg aatgatttta
     1381 atgccttcaa tggatttcat tggttctgtg gacttctcta tgacggcagg taatgcatcc
     1441 agcagggcaa gacggaaccg gaggctggtc tgctcatctg aaagcacgtt aatggcatcg
     1501 ttcagtgcgc gctgtgcttc cgcttctgcc agacctttct gacgggtggc ctccgccagt
     1561 tcaataattg ctgctgcctg gagttcggct gcttcttttt cagcctgggc gcgaaccgtc
     1621 agttcaaccg ctttcgtttc agcttcctga gcagcagcaa tcagggcaac ctgtttggta
     1681 cggtcggcct ccgcagtctg gcgggtggtt tccacatcct gctgggcctg tacggcttca
     1741 gccagtgctt tacttgcccg ggcttctgcc tgtgactgct cttccgattt tgccgccacg
     1801 gcaatggctc tggcctggct ggcgatttcg gttgcctgct gttgctcaat ttcccgtatg
     1861 gccacttccc gttcggcttc gacctggcgg gtacgcacta tctgctgtcg ttcgatttcg
     1921 gcttcctcta tcttacgttc agccagaatt cgcgcggatt ctgcttcacg acgtcgttca
     1981 gcttcaatag cagcaatact ggcactctgc tctgccgtac gggtttttac ccgttgttgt
     2041 tgctccagag tcatgaaggc ttcctgctgt tcaatctcca gacgtcggga gagtgcatcc
     2101 cggttttttt ccctgatggc cacttcaaca tcctgctcaa cttcgttacg ttcgcggcga
     2161 cgacgttctg tttcctgggt cagaagggtc aggccttcag catcaaaggc attgtcagga
     2221 ttaaaatgtt cccgtgcagt ctggttgaaa cttgtcagcg acacactttc aagctccaga
     2281 ccgtttttgg ataagtcttc cgcaacggtg ttctgcacac cctgaacaaa gttctcgcgg
     2341 gcatcctgta aatcctgcat gctcattctg gccgccgtgg cccgcagggc atcaacaaat
     2401 ttatcctcga ccagactacg cagatcttca ggtgtgagcg tgcgttgtcc cagcgtctgg
     2461 gctgcagtgg atattccttc ggctgatggt tttacccgaa gaaaaaaggc aacggcaaca
     2521 tcaacacgca tgcggtcgcg ggtaatgaga ctttcagccg ctgcacgact gacttccagc
     2581 ttcagggtat tcatgttgac gggaattgtt tcgtgaaaaa tgggcataac aattgcaccg
     2641 ccactcatta ccactttttg gcctcccaga cctgtgcgga caaaggcctg ctctgccgat
     2701 gcccgacggt acaggcgggc aaatatcagc ccgataacga acagggtgag gaccagcgca
     2761 ataacggtaa acaaccatcc cggaagatta aacatagtga gaacctctgt atatgtcggg
     2821 attaaagaac atccggccag ggattcagtt cacccagcca gcgggaaccg gaaagacggc
     2881 agacaataat cactttgtct cctcgtctga attctttccc ggcctccggc tccagcaaca
     2941 ggtaatgaac ctgcccgaag cggtctgtga agcgaccttc acagggggaa cccgccactg
     3001 cgtgataacc ggtgatggtg gccatacagc cgatgaatgc ctcctcggtg agcgccgttg
     3061 tttcatcacg gggtaaccag ggggcaatca gtctgccggt gcggtgtacg agaggacatg
     3121 tcagcagcag gcaaagtggt gcaaggagaa tgttcggggc aggagcctgc agcagatgaa
     3181 taataagacc ctgcaggaga atgccggata taccaaaaaa aactgccagc atacagagga
     3241 cgatcagcag ggggatccgt ccgatgttca gccagtccag cgcctctcct gtcagaccct
     3301 ccggtaagtc aggtgtatcc atagctgaag agagggagtg tccgcagagc agggcgataa
     3361 gttcagcgat accagtaagc agaacaaaag tcagggctgc cagatagggt gcgttatacg
     3421 cggtaagtaa cgtcagtgtc atttttcctc ctgtggcagg tcagtcaaac aacattatgc
     3481 gtgaagcttc cgcttcttta attgtcagaa gaagcatatc gtcagtcatc agaagacaag
     3541 gggatgtctg caggagggga tgataacaac agaagcctca cgccggaatg tgctttatga
     3601 caacaggaga ggacggataa taaaaaatgg gacatccaca tcagcaaacc gcgatttgcg
     3661 aaaaaaagtc agcggagagt ggctgccgtt gtggctgtcc cggtatttca gtgtaacagg
     3721 tgattgctgt gatctatgcc ccattcggct ttcggtaaac ctgaatgccc cacatgaaga
     3781 atgcgtgaca gcatgtttct gccgctgccg ttcaggctgc attcgcgtcc gtgcgcgagt
     3841 ataaaacctt catcccggaa tatctcttcc acttctgaag attcgaggga tgccggatga
     3901 attaatgaaa atgccaccgg tctgagcatt ctgcccgtgg tggaaaaggt gtctctggta
     3961 ctgttcagct gcaggatatg acgtttacct gtttgcgcac gggaaagtgt ctcactcagc
     4021 agctggtcgt tccatgcgca cgtgccaatc tgccatccca gacttctgca cgcggaaaga
     4081 gccgagcgga agcggtacag gtcgtataat gctgcgttat tgccactttc cagcagtgat
     4141 ttcagggtgt ttgctaccgg tatgtctgaa ttggtggcca gcagttttcg cagggtagtg
     4201 aactcttcag ccattcctct gaactcgcgc atctgacggg cagtgtcaag ccagttcagc
     4261 aggtaacgcc ggcgacgccg ttcgttgttt atgttgtccg gggcggtgga gagtgcttcg
     4321 agcatattct ccagtaacag aatgacaggg ttttcctggg atagaatggt cataaaggcg
     4381 tcctgaaaaa atgcccgaca gccgctctcc gtacaccctc aggtgtcacc ggtgccgggc
     4441 attgtggaaa cagaagggaa aaaaatcccg gccataaagg ccgggaaaag tcgtcaaatt
     4501 aagacagcaa ttttcatgtc aacgctccgt catcagatac caggtgcagg agcagggcga
     4561 tcctcgactc cggcagaggc tgtgcatcaa gccggaggag gtactggtaa cgcgcttttg
     4621 tcagccagag ggtggaggca gcatccagct cctccctggc tgccggattg agcgcaggat
     4681 ccgggtacac tttcatcaaa gactgaacga tatcctccct gacggttttg atttcaccgg
     4741 ccattttcac gaagttcaga aaggcactgt gacggcgaag gtgagtgaag ctatggcgtt
     4801 cggggtctgc tttcaggaaa aagtaaccgg gaaacacggc gtgtatatgt cgacggtaac
     4861 cgcagacttt atccgtgcgg cggacttttt tcagtatcag gggtgtccag ggaaggacgt
     4921 gttgttcgga aagccaggaa aagagatgtt cacggttttt gccggcaggg atgtactgag
     4981 ccagatacca gttgcgttga tgtaattcac tgatattcaa aatccggaac gctccgcgaa
     5041 aatcgatccc cgcagagggg cttcgatgtt gttgtgaacg gcgaatacat aaagcatgtg
     5101 tcggaaacag cggctgaccc tcgttcagcc cggcctgcag ttctgacagg cacatgattt
     5161 acgcactctt gtaagccttg tggacggttt cgaggcgggc gaaaaccgac aacttctgca
     5221 aacaggatta atggctggca aatatattac ataaggatgt atctgtggca agagtgaaga
     5281 taagcagtta aatagatcgt tatatttaat aaatcagcat attatgctga tttgtgtgcg
     5341 gtgtctggag gattagatcg tttaaaaatt ttaggcgatc tcttctacta aaaatgatga
     5401 aaaacacagc ataaaagatc gtttgtggaa ttgtctattt ttttgacata gttcaaaaaa
     5461 acgacgtttt ttaggtggtt aatgagaggc ctggtaaatc agagaaatcg tgtcggcttt
     5521 tcaaagcggt ggaaaagggg tatattgcgg atcgttatgc agtggctttt gggatcgtca
     5581 ggatccggga agtcagaaaa cggcaggatg cgccataagg cattcaggat gtatggcaga
     5641 aacgacggca gtttgccggt gccggaaggc tgaaaaaagt ttcagaagac cataaaggaa
     5701 aacccccata atcttcttat cgaacttggc ggaacgtaga gaagattatg ggggcctcac
     5761 agaatacggg ataagtatat atgaaaccgt accagagatt caacccttcg cagtgcatta
     5821 acgcacggca ttctgtttcc gccataagcg acagcttgtg gcggccctga accagacctc
     5881 atctactgaa agcacatacc gtcaggtaaa gaacccgaat ccggtcttca caccccgtga
     5941 agatgccggg acgctgaagt tctgcgaaaa actgatggaa aaggcggtgg gtttcacttc
     6001 ccgtttcgat ttcgccgtgt atgtggcgta tgcccgttcc cgggggctgc gtcggcgcat
     6061 gccaccggta ctgcgccgac gtgccattga tgcgctgctg caggggctgt gtttccacta
     6121 tgacccactg gccaaccgcg tccagtgttc catcaccacg ctggccattg agtgcggact
     6181 ggcgacggag tctgctgccg gaaaactctc catcacccgt gccacccggg ccctgacgtt
     6241 cctgtcagag ctggggctaa ttacctacca gacggaatat gacccgctta ttggctgtta
     6301 tattccgacc gacatcacgt tcacaccggc actgtttgcc gccctcgata tttccgaaga
     6361 ggcggtggcc tccgcacgcc gcagccgcgt ggaatgggaa aacaggcagc gcaaaaagca
     6421 ggggctggat gcgctgggta tggatgaact gatagcgagg gcctggcgtt ttgtgcgcga
     6481 gcgtttccgt agttatcagg cagagcttaa gtcccacggc atgaaacgtg cccgtgcgcg
     6541 ccgtgatgca ggcagaaccc ggcaggacat tgtcaccctg gtgaaacggc agctgacgcg
     6601 tgaaatcgcg gaagggcgtt tccggggaag cctggaggct gtgaagcgcg aaatcgaccg
     6661 tcgcgtgaag gaacgcatga taatgtcgcg gaacaacaac tacacacggc tggccacagc
     6721 ctcaccctga gttttacctc atctgaataa tccggcccgc gccggaggct tccgcacgtc
     6781 tgaaggccgg cgacgcacaa aaaagcagcg cagcacacaa aaaacagcct catcatctgc
     6841 cttccggctc gccttgctcc ccctgttttt cacgcaaaac tcgtctcaca gacgggggat
     6901 tctgcttatc cacatttaac tgcaagggac ttttccgtaa ggttacaacc gctcattcca
     6961 taaagcgcca gccgcaggcg ttacagggtg caatgtttct tttaaacccc agtactgtat
     7021 ttcctttaaa ctacttaatt agttccattt aaaaagaaaa cctattcaat gcctgtcctg
     7081 tggacaggca aatatgtacc tcccactcta acgcgcgggc cccgaacctc accgttctga
     7141 aaccacagca aaaaaacatc aggaataaaa acactacaca aacgcagcac cgtgcccacc
     7201 cctcataact gaaaagcgag gccgcccccg cccaaagggc gggaacaaca tcgcttttaa
     7261 ttatgaatgt tgtaactaaa caacaccatc gctgtcagtc ttctggctgg acgtactgaa
     7321 tacacgctcg taagcggccc tgacggcccg ctaacgcgga gatacgcccc gacttcgggt
     7381 aaaccctcgt cgggaccact ccgaccgcgc acagaagctc tctcatggct gaaagcggat
     7441 atggcttagc agggtgggaa tgggataggc gaaatctatc aatcagtacc ggcttacgcc
     7501 gggcttcggc ggttttactc cggtatcata tgaaacaatg gagtgccgcc ttccatgccg
     7561 ctggcgcggc atatcctggt gacgatatct gaatcgttat atactgtgta tatacgtagt
     7621 aatgacgagg tgataaatgg cacaggttaa tatgagttta agaatcgacg ctgaactgaa
     7681 ggatgctttt atggccgctg caaaaagcat ggaccgtaat ggctctcagt taatccggga
     7741 ttttatgcgc cagaccgttg aacggcagca taatacctgg ttccgcgacc aggttgcggc
     7801 aggacgtcag caactcgagc acggcgatgt gcttccccat gacatggtcg aatcctctgc
     7861 tgcagcatgg cgggataaaa tgagcaggaa ggtcgccggt aaatgatgga gatattctgg
     7921 actatgctgg ccagccagga caggaagcgc attcgtgagt acattgcgga gcagaacctg
     7981 atggccgcca ttgaactgga cgaacggatt ggttattcag ccagcagcct ggccgggcag
     8041 ccgtataaag gccgcaacgg tcgtgtggaa ggtacccggg aactggttat tcacccgcat
     8101 tttgttctgg tttatgaggt cgacagccag tggggaaaag tgtatatcct gcgtgtattg
     8161 catactgcac agaagtggcc atagagaggc tgtccggata taaaaacgtc tgacggagat
     8221 gcaagaacca tgaataatga tgagctggcc accagacgtg cgcaggcgat tgctgaagac
     8281 agatgtttct cgaaaggacg tctgcgcgat gaattcagaa tgaagcctgc ccccggtgct
     8341 gagccggtta agtggtataa aaacacttat ggtggcaggt tcgctgtata ccgaattgca
     8401 gattgtgttc cgatgcgtga gaaacgccca ctgacgagta aacaacttct ggctggccag
     8461 cgactttccg ttttatccag acttaacagc accagtggcc ggatggcacg gcaggcatat
     8521 gactggctga gtctggcacc gctctttctg gatactgaaa cgacaggact ggataatacc
     8581 gcggaagccc tggaaatcgg ccttactgat gcggccggtc aggtggtatt tgaaaccaga
     8641 ctgaagccca cggtcgccat cggggcgcag gccgccgctg tgcatggtat cagtgagcag
     8701 gctctctgcg gcgcaccatc atggacagat gtggcgcggc aactgcgtca tgctatcggg
     8761 ggccgaccag taattatctt taatgcccgg ttcgacatcc gcattctgaa acagactgct
     8821 gccgcacata gcgatccggc ttactggctg gaagaaatga cggtatattg tgcgatggag
     8881 ctggcagcag gatattatgg ggccaccaac cgctatggca cgatttcact ggccagtgct
     8941 gccagccagg ccggactgac ctgggaaggg ctggcacact cagcgatcgc cgacgcacgg
     9001 atgacggcag gggtggtaaa cgctattgct gcatatcatc tggaactgct gcaggaacag
     9061 gcacggctgg aaatctgacc gcctggcctg tataccgcaa tcatttacgt tatccggacg
     9121 taaatctgcc gcttgttgct gtcagcatgt taatgctaat gtatgcgtac attagcatta
     9181 atgagggatg atatgtgaga gacaaaacca gggaggccat gcggcttttt ttaggcggca
     9241 ggtgctatac ggctgaaaaa cttgaaaagg attatctggc tgaagtggct aattacagta
     9301 acgaccgctg ggaagcaccg caacgtgcct cacgcctggc tgccagcgta aagcgttaca
     9361 agacatcaga aatgctgcgc tttatttttg ctaccattgc ctacgatccc gatccagatt
     9421 taaccccgct gactgtgaga cgtttgtgca aggcattgtt cggcagaacc ggaagccagt
     9481 ggctggttgt tgaagttttt ggggagaagg ggcgtcagca ccgcagcgcg gacagcaatc
     9541 cggagatggt ggaaaaaatg gctgccagat atcgtcatgc cgccgaacta cactggtccg
     9601 caacgctggc agaaattgaa cgggtaaagc ggctttatca gacgaaaatt aagaaatcca
     9661 aaaaagaagt gggttgactg ccattgttat tcttctcctt catcattaat gctaatgtat
     9721 gcatacatta gcattaatga tggtgcgttg aacgtacaat aaggggtccg cttggaattc
     9781 ggaaattttc ccacgctaag agaattgagt tgaacgagtg gttacatcca ggccagctaa
     9841 ctcactgaac gtaaagactg tacgaaatgt tccgaccgtc cgataaccat ttggcttact
     9901 tccgtacatt tccttgcgta gatgtacaga attgtacgtt ttcgtacggt tggccctcaa
     9961 accccatcct tgagtagtgg cataagaaaa cggctccctg ttgtggaagc cgttatcagt
    10021 tgcacgatta gcattggagt ttaaaggata cctttctgag aaaatgtgat ccgggttgct
    10081 tcatttaaat ccatatattt tagggtgtca ggagatgccc agactatatc ctgaaactcc
    10141 tcattaaatg taactgtacg gtttgtactg atacagtcga atattaggta aatcatgtaa
    10201 atgtcctcgt gggtaccatc cgggtacgtt tttgtacgga tatcatcacg aaaggcccag
    10261 ggttttacgt cagtgatatg cagttctttt cccagttctt cctttatttc acgcctcaat
    10321 gctgtttcca tagtctctcc gggttccatt cctccaccgg gcagtgccca ttggcccggg
    10381 aatactccgc gatcagaggc cattttacaa agtagaaact caccttcatt ttgtatcaca
    10441 gggcacacta ttactctctg gcgcatttta tgtcctcaac atcatggctt tttggataac
    10501 cttatcagaa aaatcatcaa tgtcatccgg gtgagcacat accataatcc gctcacgaag
    10561 gtgtaacgga agattatcag taaaatgttg atattccgca tgccatcctg tgatccattt
    10621 tatcttcatt tcctcatctg gaaattcttc aggccgtaca ccatgaattt catcatcatt
    10681 gagatgcatg agtgaatgtg cgatgggata attaatgaat gctataatgt ctggctctgt
    10741 aagtaattca tttattctac tccagagcac catgtatgca tcatattgtg ctttatttat
    10801 atattcggac ttcagcatga agttgataaa aatataatcc tcaatatggg atctctccat
    10861 aataaggtta aaccctttat ccagccaaga ttttatcagt aatgctcgtt gtatcataaa
    10921 attaagctgg attagataac cgtagttact ggtatcgtca aaaaggtaag gaaggagagt
    10981 gtggtggaca cttttttcat ctatcgcgac agtgtctgga tctaccttaa ataactcctt
    11041 tgacatgcgt ctcagaagag tacttttacc tacaccggaa ttacccgaca gacatatata
    11101 cagaggattt cgagttttat tcttctcttt tttatctgct gaccaaggat ccatatacaa
    11161 gtaattatat ctcatactgt aaactccatt ttgtccaacc aaaagcggtt attcttacat
    11221 ttttgcatga tttcattatt tccgaaaatt gcactgatta tatgatcttg tgaatctgtc
    11281 agcatatttc ccaacagagt atagttttct ccatcaggga taataatgtc aaaattgggt
    11341 tccataacaa cattggcttt gtcccgttct tcgaaagttg cccaactaat gggcatgtta
    11401 gggtgttgtt tttttagcat taccatttta tctttaaata tatccgtatt gatttccagt
    11461 ttatctcttt tttcttttcc gactcctaga ggacagaact ggtaaaatcg ccaagcttgg
    11521 atcttatagg gcagagaatc aacccagcaa acaatatcac tgagatgatt aatgttagac
    11581 tgagatacga cggtcgtgag ttttatatta attcgattat tattttgggg taaccatgtt
    11641 aatatatttt tgataaccct gtgttggtta ctcattttat cactgcgaat ttctgaattg
    11701 acaagactgt caattccatc aattggtatt cctacccagt ctaacaccgg atttaaccga
    11761 gcatgatcca taataaatcg atgcccgacc gtggagagat atgtcataaa acccagttgc
    11821 ttagcgtatt caatcagatc aatgaaatca ctccgggatg taggttcccc tccggtaaaa
    11881 ccgatacgac ggaatcccat tttatacatc cgctcaatta cattacgagc actagcaaga
    11941 gaagagtcct gaattccagg tgtacgaaaa cacagatcac atcctaggtt acaatgtgaa
    12001 gttattctga aatctaaaag aagggtgcta atatccataa caagtcctct gactatgata
    12061 catcaataac cattaaaaca taatggttat tgcagaccat gacaaagcgc aaccttcggc
    12121 ttaccaggga tataatatct gccagtaatc agagcagtgg gttgcgcaag gaactcatga
    12181 atgatgagtt ccggtaatcg cattactgga agcccatgat cggagggacg ctatcagtca
    12241 caccagtgaa aattgtgcac agaagcattt tcatcactga caggccgtat gcctcacaag
    12301 acgggagata actggagaat atttagttct tctaccggct tacgccgatt ttactcctgt
    12361 gttatatgta accattgtgt gccaccttcc atgcgctggt gcggcatcag agtaaaaaat
    12421 cagcattaaa tttatttgca aatgtatgag taatacagac ttgttaacgg gtcctttcag
    12481 atgtatcttt aagacttatt aaacagtctt aaaaggtgca ttatgcctaa catcattctg
    12541 tctgacatca gtgcaagtgt cagtgaactg aaaaaaaatc cgatggcgac agtcggtgct
    12601 ggtgatggtt atccggtcgc cattctgaat cgcaatcagc cggcattcta ctgtgttcct
    12661 gctgaactgt atgaaaggat gcttgatgct ctggatgacc aggagctggt gaaactggtt
    12721 actgaacgta gcagtcaacc tctgcatgat gttgacctgg acagttattc atgacttata
    12781 cgataaaatt cagggatgat gcgcttaaag aatggctgaa actggataag accattcaac
    12841 aacagtttgt gaaaaaacta aaaaaatgta gcaaaaatcc tcatatactg tctgcaaaac
    12901 tacgtgggct aaaggactgc tacaaaatta aacttcgtgc atcgggattt cgtctggttt
    12961 atcaggtgat tgatgatatg ttaattattg cggttgttgc ggtaggtaaa cgagaacgta
    13021 gtaacgttta ttatctcgcc agtgagagaa tgagatagta acataaatag cctttcccgc
    13081 agataactct cagaattgtt attctctctg tggcctcgcc tgaggcggaa aaagaaaagt
    13141 tttgttgaga gaatgatttt acctgccggg atgagtctgg ggttatttat attaccagac
    13201 aaattgaaac ggagggacat gtttgatgac aatacttgtg acggcacagg ctcaactcat
    13261 agttggtgag gctatggcga taaaaagttt agctcctgat ggaatgttgg ccgctgtatt
    13321 tgaagacgac gggaataccg gatatttcta tgcacttgat gagtccgttg aaggtaaccc
    13381 tgtcagggat gctgtgcata tatataacgt tgaagacatt tcagatgctc acatcccctc
    13441 tgatgtgaag attggctggt ctgaggattg cctgaagtgt gttctgctga taaacggata
    13501 tccacatggg gtattcgatt ttgaggggaa gaacggctac tgtcgaagtg gattcccgcc
    13561 accggttaat catgaatggt ctgttcaggg gcattcatgg gatgattacg tcgataattt
    13621 cttccggtga ttgaaccgaa aaattacctg acggacaagt ctggggccga atcgtgatga
    13681 ttcatcatgg ccgtttatag ttcagcagtc ttgttctttt gctgtcagtg ggataccagg
    13741 aaaataaccg attgttacca gacagaataa ccatcataag acgtcacaag acggtgacgc
    13801 ttacatagtt ccggggagtt aaacaatata ccctgacttt gtaatcattt gaattatatc
    13861 cgtaacgtgt aacttcctgc cgtaacaccg gatttaagat attcaaactt tatctttata
    13921 ccgatattaa gagacacaat gcgaaaaata attgctcatt tcaagatagt tttaacctta
    13981 cttctgcccg caacagtatc tgcccagtcg atagagtggc aatcctgtat gaccactcca
    14041 ttcagtaact ggtttggtga ggaaaaaccg tctcctgact tactatgtgg ttatttgtct
    14101 gttccattaa aatatacaga cacaggcgga gatagttctt ctgtaaaaag acagcaggta
    14161 aaactggcgc tgacaaaatt gccagcaaaa agcaggcata aagggagtat cattataata
    14221 agtggtggac caggattacc tggcattaat ccttatatta actttgactg gccggtaaca
    14281 aatcttcgcg aatcatggga tattattggc tttgatccac ggggagtcgg ccagtccatt
    14341 cctgcaataa actgccagca atcagataca gggattcagg aaaacataac tgaaaagcaa
    14401 cgggtattaa ataaaattaa cagctgtatc cataataccg gaactgaagt tattcgccat
    14461 attggttcta acgaggcggt atatgatatt gatcgtatca ggcaagccct gggtgacgag
    14521 caactgacag ctgtggcata ttcgtatgga acacaaattg cagccttata tgcagaacgt
    14581 ttcccatcca gcatcagatc tatcgttctt gatggggtcg tcgatattga tgacctgggg
    14641 gacaacttca catggcaact caaacaagca cagagttatc aggaaacgtt cgatcgcttt
    14701 gcatcctggt gtgcacgtac aaaaagttgc ccactttctt cagtcagaga taaggcaata
    14761 actcagttcc atgagctgtt atcaaaattg catcacaagc ctttattaga cagcaaggga
    14821 gaaaatatat cttcagatga actcatatta ttaacaacag accttttgtt atggcgttca
    14881 tcatggccaa cccttgcaac tgccatacgt cagttctctc aggggattgt cagtaatgaa
    14941 attgaaactg cgctcagtac tcctatagcc tcagaagagt caagtgatgc tctgggggta
    15001 atcctttgtg ttgatcaggg gggggagcaa ttaaccccag aagagcgaaa atcccgaaaa
    15061 gatgctcttg cgaatgcctt cccggctatt aactttgaca atggacgttc cgattcacct
    15121 gatttttgtg aattatggcc aatacatagc aacctgaata aaacccggct gaaaaataca
    15181 gttctgccct ctggtttact gtttgtagca cacaaatacg atccaacaac accatggatt
    15241 aatgctcgta agatggcaga gaaattttcc agcccgttgc tgacaataaa tggtgatggg
    15301 catacattag ctcttaccgg agtcaattta tgtgtagata aggccgttgt tcgtcatctt
    15361 atcaccccta tgaaaatggg aaatatatcc tgtccaggaa atgctgaaat agatataaaa
    15421 tagtttaaaa attgcccgcc atttgctcct gataccggac atattttgca ttgggagcaa
    15481 aatgcgttca gtgcgatcac ggcaccagaa acgtcaaact ggatacacgt aaaaaatgtc
    15541 gcgatcgcgc aaaatcaagc cattactctc gggagtgctt aaataatacg cacaatatac
    15601 aacgctctct tctctacagt ctgtttcctg taatatcctg cggttatttt gatttctcag
    15661 aaatattttc ctgccgggcc ttcatactca gccacctctg ctctttcgcc gccagccagc
    15721 gcctggaatt ttgagcatca cattccgata cttccccctc tggttgacca tccaggttat
    15781 aacggggagt tcctgcaaca gaggttctct gcaggtagat ccggcgactg gtaatgcagg
    15841 atactgcaca attccattcg tccagggtca gcgctgtttc cggatgtgct gccagatatg
    15901 tagtaatgtc acgggttatg cctgttttca gaggcagaaa accgccccct tcggcacgaa
    15961 gttgaggaaa cagcgctttc accttctcca gacaatcgtg agtgaactca ctggtccagt
    16021 gggatggtct tggaaagcgt tgtcttttct ttttcttctt cctggctggc gcttcgggga
    16081 tatgagtttc gacggcaggt gaactgattc cggaggagac tgattctgtc gcctgttcag
    16141 tgagtgcagg cgcgataaca tctttttttt cggctaagga aggcggtgaa aaagagcgac
    16201 gttttttcac cacaatgacg ggatttttct tttcttccag atcttttc
//
LOCUS       DAZGAP010000125        16220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGAP010000125 DAZGAP010000000
VERSION     DAZGAP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.126"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..775
                     /gene="pdeL"
                     /locus_tag="ACHLAM_003476"
     CDS             <1..775
                     /gene="pdeL"
                     /locus_tag="ACHLAM_003476"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO7854948.1"
                     /translation="HIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHP
                     QTGIIPPDQFIPLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFL
                     AAGFEKECLNLVKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFG
                     TGYATYRYLQAFPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVET
                     QEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(817..1749)
                     /gene="yahB"
                     /locus_tag="ACHLAM_003477"
     CDS             complement(817..1749)
                     /gene="yahB"
                     /locus_tag="ACHLAM_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7854949.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(1841..2338)
                     /gene="yahC"
                     /locus_tag="ACHLAM_003478"
     CDS             complement(1841..2338)
                     /gene="yahC"
                     /locus_tag="ACHLAM_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7854950.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYFGMVWAFFAALAAGWLASVSGISGFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            2596..3201
                     /gene="yahD"
                     /locus_tag="ACHLAM_003479"
     CDS             2596..3201
                     /gene="yahD"
                     /locus_tag="ACHLAM_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO7854951.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            3241..4104
                     /gene="yahE"
                     /locus_tag="ACHLAM_003480"
     CDS             3241..4104
                     /gene="yahE"
                     /locus_tag="ACHLAM_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7854952.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQCGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4094..5641
                     /gene="fdrA"
                     /locus_tag="ACHLAM_003481"
     CDS             4094..5641
                     /gene="fdrA"
                     /locus_tag="ACHLAM_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7854953.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLRNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RMQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            5641..7059
                     /gene="yahG"
                     /locus_tag="ACHLAM_003482"
     CDS             5641..7059
                     /gene="yahG"
                     /locus_tag="ACHLAM_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7854954.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPTAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7085..7339
                     /locus_tag="ACHLAM_003483"
                     /pseudo
     CDS             7085..7339
                     /locus_tag="ACHLAM_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7389..8339
                     /gene="yahI"
                     /locus_tag="ACHLAM_003484"
     CDS             7389..8339
                     /gene="yahI"
                     /locus_tag="ACHLAM_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO7854955.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8349..9731
                     /gene="yahJ"
                     /locus_tag="ACHLAM_003485"
     CDS             8349..9731
                     /gene="yahJ"
                     /locus_tag="ACHLAM_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO7854956.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVQIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGNVAG"
     gene            10027..10437
                     /locus_tag="ACHLAM_003486"
     CDS             10027..10437
                     /locus_tag="ACHLAM_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO7854957.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            10691..11674
                     /locus_tag="ACHLAM_003487"
     CDS             10691..11674
                     /locus_tag="ACHLAM_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7854958.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            11723..13207
                     /locus_tag="ACHLAM_003488"
     CDS             11723..13207
                     /locus_tag="ACHLAM_003488"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO7854959.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            13200..14171
                     /locus_tag="ACHLAM_003489"
     CDS             13200..14171
                     /locus_tag="ACHLAM_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7854960.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            14168..15124
                     /locus_tag="ACHLAM_003490"
     CDS             14168..15124
                     /locus_tag="ACHLAM_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7854961.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            15211..>16220
                     /gene="yahK"
                     /locus_tag="ACHLAM_003491"
     CDS             15211..>16220
                     /gene="yahK"
                     /locus_tag="ACHLAM_003491"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO7854962.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSELKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DV"
BASE COUNT         3958 a         3886 c         4450 g         3926 t
ORIGIN      
        1 ccatatcgtc acgcctgaag ccatcagtct ggcgttggaa aaccatgaat ttaaaccgtg
       61 gatccaaccg gttttctgcg cgcagactgg ggtactgacg ggctgtgagg tgcttgtccg
      121 ctgggaacat ccacaaacgg gaattatccc accggatcag tttattcctc tggcggagtc
      181 atccggtctt attgtcataa tgacccgcca gttgatgaaa cagactgcgg atattctgat
      241 gccggtaaaa catttgctgc cggacaattt ccatattggc atcaacgtct cggcgggttg
      301 ttttttggct gcgggatttg aaaaagagtg tctgaacctg gttaagaaat taggtaacga
      361 taaaatcaaa ctggttctcg agctaacgga acgtaaccct attccggtaa cgccagaagc
      421 cagagcgata tttgacagcc ttcatcagca caacattacc tttgcgctgg atgactttgg
      481 tacgggttat gcgacctatc gttacttgca ggcgttcccg gtcgatttta ttaagatcga
      541 taagtcattt gtgcaaatgg cgagcgtgga cgaaatatcc ggtcatattg tggacaatat
      601 tgtcgaactg gcgcgtaagc ctggtctgag tatcgtggcg gaaggggtag aaacccagga
      661 gcaggcggat ttaatgatcg gcaaaggcgt tcactttttg cagggctatt tgtactctcc
      721 gccagtaccg ggtaataaat ttatctctga atgggtaatg aaagcaggtg gttgatgtaa
      781 accgctattc acagcgcatc gggaggttgg cagcgattaa ttctcccgat gcagtaaatc
      841 ctgatagatc cccgttaata tgccatttgg cgaaaattgt tttttaatcc actgcgtcac
      901 ctgacccgta gcagaatgct gagttgcgag taacatgcgt gaatcctggc gcggattatg
      961 gatttgtcgg gtaaccagca gggattgcgc catcgcctca cggaccatgt aatccggcaa
     1021 aaaaccaatt ccttcaccta aaatctggca ctgacatttg gtgttgaaat ctggcaccag
     1081 aatcgactcc tgcccatgca gcaaccagcc cactttttta ttaatcgtat gcgcggtgtc
     1141 ctccaccatg atattggggt aaagacgcag ctggctttcg gcgatcggct ccggcacaaa
     1201 ggctaatggg tgatccgggg cgatagcaaa agcccagcga attgcgccaa tctccgtgta
     1261 atcaatgccg ccgccgtcca gcagcgtatc gggtgcgccg atagcaatat tcgcctgatt
     1321 attaataatc gcatcccaga cgccgttata cacttcggtc gtcacggtaa tctggcaggt
     1381 tggaaactgc tttttcagta cctgtaataa gcgtgcggtg tgtttgggcg tataaagcag
     1441 ttggttaata caaatgcgca cccgtgcttc tataccctgc gaaatagtat cgatgctgcg
     1501 tttgatggcg tgaaaatcat tcagcaggtc ggtagctttg cggaaaaaat aacgcccgga
     1561 ctccgttaac tcaatgcttc gggtactgcg agtgaacagc accacatcca gccctgtttc
     1621 cattcgcttg atggtgtagc taatggcgga agtggttaaa cccaactctt ctgcggcttt
     1681 actgaaactg ccaaaacgcg cagcggtagt aaatgccaac agattctctt cggtaaaaat
     1741 tgagttcatt aaaccattcc cgccattatc gaatatgaat ttctccagct atgactgtag
     1801 aggtatcggt taaagataga taatcatttt tgaataactt ttaatacccg tcgcgtttag
     1861 tcgtcgcttc gcttaatttt tgtccgccat attctgaaat tatccccacg cagttacccg
     1921 ccagaaaacc cagtaatgtc accgtccagt tcatcccgct ggcaaaaaat agcgtcatgc
     1981 cgagaaagcc tccgggaata aaagacagta aaccgaaacg tccttgccag accaccaccg
     2041 ccgagaaggg tacagtcata atcacgctcg cccagaaccc ggaaatccca cttacagagg
     2101 ccagccagcc ggcagccagc gcggcgaaaa aggcccacac catcccgaaa taattaaccg
     2161 ccagacttct gataaacccc gatttaccgc cgcctgcggc ataaaaactg caaaaggcga
     2221 cgaagccaat ggttcctaat aattcccatc cctgagagag cccgacatgt gaagacacta
     2281 actgccaaag ccctgcacaa ataccgacgg tgacacccgt tgccgtgagt ccgttcatct
     2341 tgttcccctc tggttagaca tggacgaaag ctactcttac atgtgcagac tatagagaaa
     2401 taaaacctga ttgagtgaaa gggtatcggg tcaaagaaac aaatattgca ctaccgcaca
     2461 ctgcgaaaag attgttgaac atcattcaac aaaaaggcga aatacggcga attaaattta
     2521 agcgggttct tttatcagat tatcgttgct attctcaatt catcgaaaac agccacaaaa
     2581 accaggggaa tgtgaatgac tataaaaaat ctacctgccg actatttatt ggctgcgcaa
     2641 cagggtgata tcgataaagt aaaaacctgc cttgcgctgg gtgtcgatat taatacctgc
     2701 gatcgtcagg ggaaaacggc aattacgctg gcaagtttat atcagcaata tgcttgcgtt
     2761 caggcattaa ttgatgccgg agcggatatt aataagcaag atcatacctg tttaaatcct
     2821 tttttaatta gctgtctgaa cgatgattta acgctactac gaattatttt accggctaaa
     2881 cccgatctta attgcgtcac ccgttttggc ggtgtcggcc tgacgcctgc ctgtgaaaaa
     2941 ggccatttaa atattgtaaa agagcttttg gcgcatacgg agattaacgt taaccagacc
     3001 aaccatgttg gctggacgcc gctactggaa gcgattgtgc ttaatgatgg cgggattaaa
     3061 cagcaggcga ttgtccagtt attgctggaa cacggtgcca gcccgcacat gacggataaa
     3121 tatggcaaaa cgccgctgga actggcgcgg gaacgaggct ttgaagagat tgcgcagtta
     3181 ctgattgccg caggtgcata aaccgggagg cttgctatca acacaccaga aagacggtgt
     3241 gtgtgggcgc taactgcgga tgctgatttt ctggcgcagc gggggcaagg acaggttgaa
     3301 caggtctttg ccagagcggt aaatatcgca cttccggccc gccagcagtt gctgacgctg
     3361 ctttgtgaag agtacgacaa tgcgccaaac agttgtcggt tggcactcac tcactttgat
     3421 ggtctgttcc ggcatggtga taaggttcag tttgacgatc aaggcattac ggttggtcaa
     3481 catcttcata tagagatgag tcgttgtcgg cgttggctgt ccccaacctt gcaaatggcc
     3541 gctgtgaatt ttcaccttat cgcctggcaa cagtggcatg acattattca tcagcacctg
     3601 ggggaaaatg aaaccctgtt taattattgc ggcgataatc cgttttatca ggcgttaaat
     3661 aaagaattac atattaaacg acgggcagtt attcaggccg taaacgataa acaaaatatc
     3721 gcctcagcag tcgccagtat gatgggctta gggattggcc ttacgccatc agccgacgat
     3781 tatttaacag gtctggcgct tattttattt atttccggac atccggcgga aaaatacaaa
     3841 gaggaatttt atctcggtct gcaatgcggc agaaataata ccacgttatt aagtgccata
     3901 acgctggaag ccgcattaca acaacgctgc cgggaaaata ttcatcgttt tattcataac
     3961 attatttatg acatccctgg gaacgcaact caggcaatag aaaaaattaa acatattggc
     4021 tccagttccg gctgcgacat gctgtatggc atggccgatg gttgtgcgct gagccaaacc
     4081 tacggaggga attatgtcag ttaaaatagt cattaaaccg aatacctatt ttgattctgt
     4141 ctcgctgatg tctatctcca cgcgtgcaaa taaactcgac ggcgtcgagc aggcatttgt
     4201 ggcgatggcg accgaaatga acaaaggcgt gctgaggaat ttaggactgc tgacgccgga
     4261 actggagcag gcgaaaaacg gtgacctgat gattgtcatc aatggtaaat cgggtgcgga
     4321 caacgagcag ttgctggtgg agattgaaga actgttcaac agcaaagcgc aaagtggctc
     4381 gcacgaggcg cgttacgcca ctattgccag cgccaaaaag catatcccgg aaagtaacct
     4441 ggcggtgatt tcggtcaacg gtctgtttgc cgctcgcgaa gcgcgtcagg cgctgcaaaa
     4501 cgatcttaac gtgatgctgt tttccgataa cgtctcagtt gaagatgaac tggcgctcaa
     4561 acagttggcc cacgaaaaag ggctgctgat gatggggcca gactgcggta cggcgattat
     4621 caacggcgcg gcgctctgct ttggtaacgc cgtgcgtcgc ggcaacatcg gcattgttgg
     4681 cgcatccggc accggtagcc aggaactgag cgtccgcatt catgaatttg gcggcggcgt
     4741 ttcgcaactg atcggcaccg gcgggcggga cctgagcgag aaaatcggcg gcctgatgat
     4801 gctcgacgct atcgggatgc tggaaaacga tccgcaaact gaaatcattg tgcttatctc
     4861 caaaccgcca gcgcctgcgg tggcccgcaa agtgctggaa cgcgcacgcg cctgccgtaa
     4921 gccagtggta gtctgcttcc tcggtcgtgg cgaaacgcca gtggatgagc aggggctaca
     4981 gtttgctcgc ggcaccaaag aagcggcact aaaagcggtg atgctctccg gtatgaaaca
     5041 agaacatctc gacttgcata ccctgaacca gccgttgatt gcggatgtgc gtgcgcgtat
     5101 gcaaccgcag cagaaataca ttcgtggcct gttctgcggc ggcacgctgt gtgacgaaac
     5161 catgtttgcg gtgatggaaa aacatggcga tgtctacagc aacattcagc ccgatcctga
     5221 attccgcctg caagatatca accgcagcgt caaacatacc ttcctcgact ttggcgatga
     5281 cgattttacc aacggcaagc cgcatccaat gattgacccg accaaccgca tcagccggtt
     5341 gatcgaagaa gcgcgcgatc cagaagtggc ggtgatcgtg atggattttg tgctcgggtt
     5401 tggatcgcat gaagatccgg tcggttccac catcgaggcg atcaaagagg cgaaagcgat
     5461 cgccgctgcc gaagggcgcg agttgatcat tctcgcctat gtgctgggta ccgatctcga
     5521 tacgccatcg ttagaacaac aaagccagat gttgcttgat gccggagtga ttctggccag
     5581 cagcagcacc aataccggat tgctggcgcg tgaatttatc tgcaaagggg aggaagcctg
     5641 atgagcccgt cactgtttag ccaaccgttg aacgttatta acgtcggtat cgccatgttt
     5701 agcgatgatc tgaaaaagca gcatgtagaa gtgacccaac tcgactggac gccgccgggg
     5761 cagggcaata tgcaggtggt gcaggcgctg gacaacattg ccgattcccc gctggcggac
     5821 aaaatcgccg ccgctaacca gcaggcgctg gagcgcatta tccagtcgca tccggtgctg
     5881 attggttttg atcaggcgat taacgtggtg ccgggtatga cgccgaaaac cattcttcac
     5941 gccgggccgc cgatcacctg ggaaaaaatg tgtggcgcga tgaaaggcgc ggtcaccgga
     6001 gcgctggtgt tcgaagggct ggcgaaagat ctcgatgagg cggctgaact ggctgcctcc
     6061 ggggagatca ccttctcgcc gtgccacgag cacgactgcg tgggttcgat ggcgggtgtt
     6121 acctcggcct cgatgtttat gcacatcgta aaaaacaaaa cctacggcaa catcgcttac
     6181 accaatatga gcgagcagat ggcgaagatt ttgcgtatgg gcgctaacga ccagagcgtg
     6241 attgaccgcc tcaactggat gcgcgatgtg caggggccca tactgcgcga cgcgatgaaa
     6301 attatcggcg aaatcgatct gcgcttaatg ctggcgcaag cgctgcatat gggcgatgag
     6361 tgccataacc gcaataacgc cgggacgacg ctgctgattc aggcgctgac gccagggatt
     6421 attcaggcgg gctattccgt cgaacaacag cgggaagtgt ttgagtttgt cgccagcagc
     6481 gactacttct ccggcccgac gtggatggcg atgtgtaaag cggcgatgga tgcggcgcac
     6541 ggcattgaat acagcactgt ggtcaccacc atggcgcgta acggcgtcga gttcggcctg
     6601 cgggtcagcg gcctgccggg gcaatggttt accggcccgg cgcagcaggt gatcggcccg
     6661 atgtttgccg gttataagcc ggaagattcg gggctggata tcggcgacag cgccatcact
     6721 gaaacctacg gcattggcgg atttgccatg gcgaccgcgc ctgcaatcgt tgcgctggtg
     6781 ggcggcacgg tggaagaggc tatcgatttc tcccgccaga tgcgtgaaat cactctcggt
     6841 gaaaacccca acgtcaccat tccgctgctc ggttttatgg gggtgccgac ggcaatcgac
     6901 atcacccgcg tgggcagtag cggcattctg ccggtgatca acaccgccat cgcccataaa
     6961 gatgcgggcg tcggcatgat tggcgcgggc attgtgcatc caccattcgc ctgcttcgag
     7021 aaagccattc ttggctggtg cgaacgttac ggcgtctgag ttacatgttt atgccggatg
     7081 cgacatgaat gtcttatccg gcctacgaat ggcgcggaaa tctgtaggcc tgataagcgt
     7141 ggcgcttcag gcattggcca ccacagccgg atgcgacgtg aacgccttat ccggcctacg
     7201 aatggcacgg aaatctgtag gcctgataag cgtagcgcat caggcattgg tcaccacagc
     7261 cggatgcggc gtgaacgcct tatccggcct acgagtggcg caagaatctg taggcctgat
     7321 aagcgtagcg catcaggcag tctggcgttg gtcataaccc catcaccctc tgtagcggac
     7381 ataaaaatat gaaagaactt gtggtcgttg ccattggtgg caacagcatt atcaaagata
     7441 acgccagcca gtcgattgag catcaggcgg aggcggtgaa agccgtcgcc gatacggtgc
     7501 tggaaatgct ggcttccgat tacgacattg tgctgaccca cggtaacggg ccgcaggtcg
     7561 ggctggattt acgccgcgcg gagattgctc acgagcgcga agggctgccc ttaacgccgc
     7621 tggcaaactg tgtggcggat acgcaaggtg gcattggcta tctgatccag caggcgctga
     7681 acaaccggct ggcccgccac ggcgagaaaa aagccgtcac cgtggtgact caggtggaag
     7741 tggataaaaa cgatccggga tttgcccatc ccaccaagcc tatcggcgta ttcttcagtg
     7801 aaagccagcg tgacgaatta caaaaggcaa accctgactg gcgttttgtt gaagatgccg
     7861 gacggggcta tcgccgcgtg gtcgcctcgc cggaaccgca acgtattgta gaagcacctg
     7921 ccattaaggc cctgatccaa caaggctttg tggtgattgg cgcgggcggc ggcggaattc
     7981 ctgtggtgtg cagtgaagcg ggagattacc aaagcgtgga cgcggttatc gacaaagatc
     8041 tctctaccgc gctattggcc cgtgaaattc acgccgacat tctggtaatt accaccggcg
     8101 tcgaaaaagt gtgtattcac tttggcaaac cgcagcagca ggcgctggat cgggtggata
     8161 ttgccaccat gacccgctat atgcaggaag ggcatttccc acccggcagc atgttgccaa
     8221 aaatcatcgc cagcctgacg ttcctggagc agggcggtaa agaagtgatt atcaccacgc
     8281 cggaatgcct gcctgcggcg ctgcgtggtg aaacgggcac ccatattatt aaaacgtaag
     8341 gacgtaagat gaaagaaaac aatagccgcc gtgagtttct gagccagagc ggtaagatgg
     8401 tcaccgccgc cgcgctgttt ggtacctctg tgccgctcgc ccatgcggca gtctctggca
     8461 caatccattg cgacgcgaat aacaccatga aaatcactga cccgcactac tatctcgaca
     8521 acgtgttgct ggaaaccggt tttgactacg aaaatggcgt ggcggtgcag acccgcacgg
     8581 cgcgccagac tgtggaaatt cagaacggca aaattgttgc cctgcgcgag aacaatcagc
     8641 atccggacgc cacgctgccg cactatgacg ctggcggtaa gctgatgctg cccaccaccc
     8701 gcgacatgca tattcatctc gacaaaacct tctatggcgg gccgtggcgc tcgctcaatc
     8761 gtccggcagg caccaccatc caggacatga tcaaactcga gcagaaaatg ctgccggagt
     8821 tgcaaccgta cactcaggaa agggcggaaa aactgattga tttattgcag tcgaaaggca
     8881 ctaccattgc ccgcagccac tgcaatatcg aaccggtttc cggcctgaaa aatctgcaaa
     8941 atttgcaggc ggttctggcg cgacgtcagg cgggcttcga gtgtgaaatt gtcgccttcc
     9001 cgcagcacgg tttgctgctg tcgaaatcag aacccttaat gcgcgaagcg atgcaggcgg
     9061 gggcgcatta cgtcggcggg ctggacccga ccagtgttga tggcgcgatg gaaaaatccc
     9121 tcgacaccat gttccagatt gcgctggact acgacaaagg cgtcgatatt cacctgcacg
     9181 aaaccactcc ggcaggcgtg gcagccatca attatatggt tgaaacggta gagaaaacgc
     9241 cacagctgaa gggcaagctg accatcagcc acgccttcgc gttggcaacg ctcaacgaac
     9301 aacaggtaga tgaactggcg aaccggatgg cggcgcagca aatttctatc gcctcgacgg
     9361 tgcagatcgg cacgctacat atgccgctta aacaactgca cgacaaaggc gtaaaagtca
     9421 tgaccggaac tgacagcgtt attgaccact ggtcgcctta cggcctgggc gacatgctgg
     9481 aaaaagccaa tctctacgcg cagctctata ttcgtcctaa cgaacagaac ctctcccgtt
     9541 cgctgttttt agctactggc gatatattgc cgctcaacga aaaaggcgag cgtgtgtggc
     9601 ctaaagctca ggatgacgcc agctttgtgc tggtggacgc ctcctgttcc gccgaggcgg
     9661 tggcgcgtat ctcgccgaga accgcaacgt tccataaagg gcaactggtg tgggggaatg
     9721 tggcaggttg atgcgcattt gatatgtctg caacggtgaa cagtaagaga tttaagcccc
     9781 agggagtggg gcgggaatgg tttgttaatg aatgatcctg aactttataa tctcccggca
     9841 taatttccag gggattattt gagagaaata cacaaaagtg agttttaaca aaggtattta
     9901 tcaagccata aatgtgacct gcttcggata aaaaagatta aacataggaa aaccaatgat
     9961 actcttgctg atggttacca acagtaatta agatgtgatt ttttccgtaa tcgaaaaaat
    10021 attgccatga ccataaaaga aaaattctca caaaaatatc cccatgcttc cttttgtact
    10081 tttggcgatt cagcagcgtt ggccgatcac ctcgcaacgt tgattgcgac tggtgttaaa
    10141 acagcttcct gtggttcgct ggcgggatgt attgaagaca acgcatttcc gctgattggt
    10201 gagtataaga ttgtagaaaa tagccgtggt gagcctgtct gtgttatcag agtgattggc
    10261 ctgcatttac tgcgcttttc tgatgtcacc gcagagcttg cccgtaagga aggtgaaggt
    10321 gacctcagtc ttgaatattg gcgtaatgaa caccgtcgtt ttttccaggc agaaggatgc
    10381 tattcaccag aaatggacgt tatctttgaa gaatatgccc ttattgacgt tgtgtagata
    10441 actatttttc tacgcgaaat aagctcacaa acataaagta tcgctaattt tgtttatttt
    10501 gctgtttttt acgtgatgat tgtcacactt attgttgctg cccgttttgt ccatatttat
    10561 aaccaaattt ttaatagctg cctgaaatag ggctgtttat tctgcgtaaa aatattcgaa
    10621 aattcttatt ctctccagcg cgctaatttg ggcgaacact tcctgactac cctgcaatga
    10681 ggctgaaatg atgaataaac gttttgttat caatatggtg tcttcgctgt tattaggcgc
    10741 tgcgctaatt tctgcgccgc tccaggcagc ggaaaaagtc gtggtgaata tctctaaagt
    10801 cgatggtatg ccgtggttta atcgcatggg cgaaggtgtg gttgaggcgg gtaaagcctt
    10861 tggtgttaat gcgtcacagg tggggccttc aagtactgat gctccgcaac aggttaaaat
    10921 tattgaggat ttaatcgccc gtaaggttaa tgcaattact atcgtgccaa acgatgccaa
    10981 cgtgctggaa ccggtattca aaaaagcgcg agatgccgga attgtggttc tgaccaacga
    11041 atctcccggt cagccaagtg ctaactggga tatcgaaatc attgataacg aaaaatttgc
    11101 cgctgaatat gttgagcata tggcgaaacg catggggggc aagggcggct atgtgattta
    11161 cgtgggtagt ttgaccgtgc cgcagcataa cttatgggct gacttgctgg tgaaatacca
    11221 gaaagagcat tacccggata tgcatgaagt tacccgccgc atgccggttg ccgagagtgt
    11281 ggatgactca cgtcgtacca cactggacct gatgaaaacc tatcctgact tgaaagcggt
    11341 tgtctccttt ggttcaaacg gtccgattgg cgcgggacgt gcagtgaaag agaaacgggc
    11401 gaaaaataaa gtcgcagtct atggcatgat gatcccttca caggcggcat cactgattaa
    11461 aagtggtgat atcaccgaag ggattactta cgatccggcc tctgcaggtt acgcactcgc
    11521 ggccgtcgcc agcacgttgc tgaagggtga agagatcaaa cctggattag aaatgcagaa
    11581 tctcggtaag gccgacgtag atatggataa gcgaattatc cgtttccata aggtattact
    11641 ggtcaacaaa gacaatatcg actcacttta ttaatccatt tcttcggggc gaaagccccg
    11701 attcttattg ccaggggatg ctatggaaac cttcctttcc cttcgtcata tcaacaaaac
    11761 gtttcatgcc acgcgagcgc tgcgcgatgt ttctctcgac tttatgtcgg gtgaggtgca
    11821 ctgccttgcc gggcaaaacg gctgtggtaa atcgacgttg attaaaatta tgtccggtgt
    11881 ttatcgcccg gatgaggggg cggaaattac gcttggtggg aaaaactggt caaagctgac
    11941 acccgccgct tcggtggcgc aggggattca ggtgatttat caggacctct ctttatttcc
    12001 taacctgagt gtctgggaga atatcgccgt gaatcactat caccacggtc tgtttgttaa
    12061 ccgccgtcgt ctgcgtgagg tggcgcaggc ggcaatgacc agtattaacg tcacattgcc
    12121 actggatacg ctcgtttctg agctttcaat tgcccgctgt cagctggtgg caatttgccg
    12181 tgcactggcg caggacgcac ggttgatcgt gatggatgaa ccaaccgctt cgctgaccca
    12241 tcaggaagtg caagggttat tacaggtggt gcatcaattg cgggaacgcg ggatctgcgt
    12301 ggtcttcgtc agtcatcgtc tggaagaagt gatggaagtt tccgaccgta tttcagtgct
    12361 gaaagatggt gagctggtcg ggacctttcc ggccgcagag atgaccacaa aacaactcag
    12421 ttttctgatg accggccagg agttcgaata tcaggtacga gagttgtggc agggaaaatc
    12481 cagcaccccg gtgctggagg tgcgtaattt aagtcgtcat ggggaatatc aaaatattaa
    12541 cttgcgggtg gaagcgggcg aagtggtgtc gattgttggc ctgcttggtg cggggcgcac
    12601 ggaattatgt ctgagtctgt ttggtatgac ccgaccggat gctggtgaga tcctcatcaa
    12661 cggtcagccg gttacactgc atagtaacca ggacgccatc cgccacggta ttggttatgt
    12721 ttcggaagat cgcatgtcgc gcggtttggt gatggcgcaa tctattgaag acaacatcat
    12781 tagcactgtt tttcacaagg taaaagatcg cttcggcttt ctgagtgaag ctaaagtgcg
    12841 tgacctggtg gacaggctaa tcaaggcatt gaccatcaaa gtctctgacc cacatctccc
    12901 ggtgaatacg ctttccgggg gtaacgcgca gcgagtatcg atcgccaaat ggctggcgat
    12961 tgggccacga ctgttgattc tcgatagccc gactgtcggt gttgatatcg ccaacaaagc
    13021 aggaatatac ggcattatca gcgatctggc ggctcatggc attgcggtat tgatgatctg
    13081 cgatgaaatt gaagaagcgt ggtatcaaag ccaccggatt ttggtaatgc aaaaaggtca
    13141 gatcacccat agcttcctgc ctgacagcag ttctcaggcc aggattgcgg aggtggtaaa
    13201 tggctgaact gaaaaaacgc catgaattct ggctggcatt actgattgtg gtgctgtttg
    13261 ttggccttgc ctggcgcagc gacgagtttc tgacattcgg taatttgtac gatctcgcca
    13321 ataactatgc catgttgact attctcgcct gtggtttgtt tgtggtgtta atttccggtg
    13381 ggattgatat ttcgtttccg gcaatgacca tcattgcgca atacggcatg gtgctgttgc
    13441 tgcaaaaaat gggtggcaac tttgctgtcg cgtttgccct ggcgggcggc atcggcattt
    13501 tacttggctt aataaacgcc ttactggtta atcgcctacg ggtgccgtct atcatcatca
    13561 ctatctcgac gctgaatatt ttttatggcc tgctgttatg gttgagtaaa ggtgtgtggc
    13621 tgtacgactt tccgccgtgg tttgagcagg gggtaatgtt gttcaagtac accgatgctg
    13681 atggctatga ctatggcctt ggtctgccgc tgatcgccat gattacggtg gtgctgctga
    13741 cagcgtttat catgaatttc accagtgtag ggcgcaaaat ttatgccctt ggcgggaacc
    13801 gcgaatcagc cagtcgcatc ggctttagtg tgctgaaact gcaacttttc gtctatggct
    13861 atatgggatt gatgtctggt gctgcgggtg tagtgcagtc gtggacggtg atgactgtcg
    13921 cccccgattc tcttctgggt tatgagctga cagtactggc tgcggtggtg cttggtggca
    13981 ctagtttgct cggcgggcgc ggcacgttaa ccggtacttt gctcggcgtg gtgttgttgg
    14041 cagtaatgca aaacgggcta aatttattgg gagtctcgtc ttactggcaa acattgatca
    14101 caggcatcat catcgttgcc agcattagcg ccacggcgtg gagtcagcat cagaaccgga
    14161 gtctgctatg aaaaaatcgt ggcgtaataa cgttgagttt tacttgatcg gcctgctggt
    14221 gctaactgtt gccgcgttca gcattactat gccggagatt ttctggtcga taagtaattt
    14281 ccagtccgtc gcttcgcaaa tgcccgtgct gggcattctg gcgttggcta tggcggtgac
    14341 catgctttgc ggtggcatta acttgtcaat tatagccact gctaacgcct gttcactggt
    14401 gatggcgtgg gtggcgactc agtatccacc gggtattgct acggttgtcg ctacgctgct
    14461 ggcgggcgct ggcgcggcgg tgataatcgg tttatgtaac ggcgtattga ttgctggtat
    14521 tcgagtgtcg ccaattcttg caacgcttgg gatgatgacg ttgctcaaag gggtaaatat
    14581 tctggtgaca ggcggtagcg ccattgccaa ttatccctca tgggtgctgt ggttgaatca
    14641 tgcacagtgg ttcggtattc cgctaccgat gtggttgttt accgcagtgg cgctggggct
    14701 gtggatattg ctggagaaaa cgccgctcgg taaatccatt tatctgattg gttctaacga
    14761 acgagcaaca ctttactccg gtattaacac tcgtcgggtg ctgatatggg tgtatgtcat
    14821 ttccgccttg ctttgtgcgg tagcggcatt tttgatgatg tcgaaactca actcggctaa
    14881 ggcctcttat ggcgaatctt acttgctagt gtcaatcctc gcggcagtac tcggcggggt
    14941 taatccggac ggcggcagcg ggcggataat tggtatggtg ctggcgctgt ttcttctgca
    15001 aattattgaa agcggcttca atattctcgg tataagtccc tacctgacga tggccttatg
    15061 gggaacgttg ctgctctgtt tcattcaggc tcgcggcatg ttggggctgg atcgggtggt
    15121 ttaaatattg gctatattca atggacgcgt tttgccgcga tgacatatca ggcatcgcca
    15181 aatacacata gctaatcagg agtaaacaca atgaagatca aagctgttgg tgcatattca
    15241 gctaaacaac cacttgaacc gatggatatc acccggcgtg aaccgggacc gcatgatgtc
    15301 aaaattgaaa tcgcttactg tggcgtctgc cattccgata tccaccaggt ccgttccgag
    15361 tgggcgggga cggtttatcc ctgcgtgccg ggccatgaaa ttgtaggacg cgtggtagct
    15421 gttggtgatc aggtagaaaa atatgcgccg ggcgatctgg ttggtgtcgg ctgcattgtc
    15481 gacagttgta aacattgcga agagtgtgaa gacgggctgg aaaactactg tgatcacatg
    15541 accggcacct ataactcgcc gacgccggac gaaccgggcc atactctggg cggctactca
    15601 caacagatcg tcgttcatga gcgatatgtt ctgcgtattc gtcacccgca agagcacctg
    15661 gcggcggtgg ctcctttgtt gtgtgcaggg atcaccacat actcgccgct acgtcactgg
    15721 caggccgggc cgggtaaaaa agtgggcgtg gtcggcatcg gcggtctggg acatatgggg
    15781 atcaagctgg cccacgcgat gggggcgcat gtggtggcat ttaccacttc tgagttaaaa
    15841 cgcgaagcgg caaaagccct gggggccgat gaagttgtta actcacgcaa tgccgatgag
    15901 atggcggctc atctgaagag tttcgatttc attttgaata cagtagctgc gccacataat
    15961 ctcgacgatt ttaccacctt gctgaagcgc gatggcacca tgacgttggt tggtgcgcct
    16021 gcgacaccgc ataaatcgcc ggaagttttc aacctgatca tgaaacgccg cgcgatagcc
    16081 ggctctatga ttggcggcat tccagaaaca caggagatgc tcgatttttg cgccgaacat
    16141 ggcatcgtgg ctgatataga gatgattcgg gccgatcaaa ttaatgaagc ctatgagcga
    16201 atgctgcgtg gtgatgtgaa
//
LOCUS       DAZGAP010000126        16181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGAP010000126 DAZGAP010000000
VERSION     DAZGAP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.127"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(85..2751)
                     /gene="cas3"
                     /locus_tag="ACHLAM_003492"
     CDS             complement(85..2751)
                     /gene="cas3"
                     /locus_tag="ACHLAM_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO7854963.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNAFCRNEMLSKQKVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFKEDAPSNINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QLADSVIFALPTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPEENN
                     SAYPLINWRGVNGAQRFDLLAHSEQLPPRFSIRPEPIYLADMLPDLRMLERMIVAANA
                     GAQVCLICNLVDVAQLCHQRLKELNNTQVDIDLFHARFTLNDRREKENRVISNFGKNG
                     KRNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEVPL
                     ATVLLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YLQTSSGKQLLDGQVYEDLSHEQQYEALALNRVNVPFTWKRNFSEVVDEDGLLWLEGK
                     QNQDGWIWQGNNIVITYTRDEGMTRVIPANPK"
     gene            complement(3110..3844)
                     /gene="cysH"
                     /locus_tag="ACHLAM_003493"
     CDS             complement(3110..3844)
                     /gene="cysH"
                     /locus_tag="ACHLAM_003493"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7854964.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(3918..5630)
                     /gene="cysI"
                     /locus_tag="ACHLAM_003494"
     CDS             complement(3918..5630)
                     /gene="cysI"
                     /locus_tag="ACHLAM_003494"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO7854965.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(5630..7429)
                     /gene="cysJ"
                     /locus_tag="ACHLAM_003495"
     CDS             complement(5630..7429)
                     /gene="cysJ"
                     /locus_tag="ACHLAM_003495"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO7854966.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPAN
                     PETSVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            7748..8110
                     /gene="queD"
                     /locus_tag="ACHLAM_003496"
     CDS             7748..8110
                     /gene="queD"
                     /locus_tag="ACHLAM_003496"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO7854967.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            8188..9459
                     /gene="ygcN"
                     /locus_tag="ACHLAM_003497"
     CDS             8188..9459
                     /gene="ygcN"
                     /locus_tag="ACHLAM_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7854968.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            9450..9710
                     /gene="ygcO"
                     /locus_tag="ACHLAM_003498"
     CDS             9450..9710
                     /gene="ygcO"
                     /locus_tag="ACHLAM_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7854969.1"
                     /translation="MSVTRNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            9727..10302
                     /gene="ygcP"
                     /locus_tag="ACHLAM_003499"
     CDS             9727..10302
                     /gene="ygcP"
                     /locus_tag="ACHLAM_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO7854970.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(10449..11309)
                     /gene="ygcQ"
                     /locus_tag="ACHLAM_003500"
     CDS             complement(10449..11309)
                     /gene="ygcQ"
                     /locus_tag="ACHLAM_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7854971.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSIRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAEINTMLPSGMLQLNIVPGALPDWLISIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(11306..12085)
                     /gene="ygcR"
                     /locus_tag="ACHLAM_003501"
     CDS             complement(11306..12085)
                     /gene="ygcR"
                     /locus_tag="ACHLAM_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO7854972.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(12063..13400)
                     /gene="ygcS"
                     /locus_tag="ACHLAM_003502"
     CDS             complement(12063..13400)
                     /gene="ygcS"
                     /locus_tag="ACHLAM_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854973.1"
                     /translation="MNTSPVRMDDLPLNRFHCRIAALTFGAHLTDGYVLGVIGYAIIQ
                     LTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWISDHIGRQKIFTFSFLLITLASFLQF
                     FATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPRRHRGVLLGAFSVVWTVGYVLAS
                     IAGHHFISESPEAWRWLLASAALPALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRY
                     FGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQT
                     IGLEDALTASLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGS
                     SLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPW
                     VLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHSVSV"
     gene            complement(13493..14947)
                     /gene="ygcU"
                     /locus_tag="ACHLAM_003503"
     CDS             complement(13493..14947)
                     /gene="ygcU"
                     /locus_tag="ACHLAM_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO7854974.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQVTAQCGVPLEVLENTLREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNHLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(15017..15802)
                     /gene="ygcW"
                     /locus_tag="ACHLAM_003504"
     CDS             complement(15017..15802)
                     /gene="ygcW"
                     /locus_tag="ACHLAM_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7854975.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGKTKEMIEKQGVEMDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
BASE COUNT         3908 a         4296 c         3941 g         4036 t
ORIGIN      
        1 tgtacattgt gcactttccc tacttaagta gggataaacc gttattggtc ttgttatcgt
       61 cattgatacc aatcattccc gaagttattt gggatttgca gggatgactc tggtcatccc
      121 ttcatcccga gtgtaggtaa taacaatatt attaccctgc cagatccatc catcctgatt
      181 ctgtttccct tccagccaaa gtaacccatc ttcatctact acttcagaaa aattacgttt
      241 ccaggtgaag ggtacattga cgcgattaag tgctaacgcc tcatactgct gttcatgact
      301 taggtcctcg tagacctggc catcgagcag ttgtttacct gaagacgttt gtaaataagg
      361 caataatggc aggctcattt ccccatccct cgttaccgca agaattgttt catcgttatc
      421 ctgcaagcta tattcttcag cccactgcag aaccttgcga gccttgaacc tcttttcaca
      481 ctcagcgctt tcaaacttat ccatgccttt gatgacccat tctggctcat ccatttcagc
      541 atcatcgtaa atgctatcca gccattgccg gtaggcatcg gggaaaaata aggatgctcc
      601 attaagttcc tcaatatgtt gctgcgtccg ccacatgact ctaacgttgc tataaatatg
      661 ctcatgtcgt ccgtaacctt cgccatcagg cagcaaaacg gtggcaagag gaacctcaaa
      721 accagcggga cgatatttgc gatgatggcg atgtaaacgg cccaatcgtt ggaaaagtaa
      781 atctgcgggg caatgctgag taattaacca atcaaaatca acgtcgagtg attgttccac
      841 gacctgggtg gcgacaagta tccgtccaac atttcgcttc ccatttttgc cgaaattgct
      901 aataactcga ttctcttttt cacgacgatc gttcagcgta aagcgcgcat gaaacaaatc
      961 tatatctact tgcgtgttat ttagttcctt taaccgttgg tggcatagct gtgcaacatc
     1021 aaccaaattg cagataagac agacttgtgc acccgcattt gccgctacga tcattcgctc
     1081 taacatccta aggtcaggta acatgtcagc taaataaata ggttctggcc gaatcgaaaa
     1141 gcggggcggg agttgttctg aatgagctaa cagatcaaaa cgttgtgcac cattcacacc
     1201 tcgccagtta atgagtggat atgcggagtt attttcctct ggatctgtat gcagaccata
     1261 agtatccaga agtttctgtt tttgtttcat tggtagagtt gcggaaagaa gaataacact
     1321 cccccccaca tcagcctgag ccttgagcac agcctccagc aagccgttca tatacgtgtc
     1381 gtaagcatga acttcatcaa caattaaaac acttcgacca attcccaaac cacggataaa
     1441 gcggtgttta actggcaata ccgatatcaa cacctgatca atcgtgcaaa cgccgatttg
     1501 cccaagaaac actttcttat tgctttgtga caaccactga caacactgaa cccatgcttc
     1561 ttcttgcccc tgctcagtaa atgcgcgtga ttttattgat tgaaaaaggt ggttaaaacg
     1621 tgaattacca tgagcaagaa taagatttgg ggaggtaaat aaacggctcg cgttcgcttc
     1681 cattctgctg agcattgcat ttgcagtagc ttgtgtaggg agggcaaaaa taacactatc
     1741 cgcaagttgt tggtcaataa gtttccaggc ataggccagc gcagtttcag ttttacctga
     1801 acccgtaggt gcctctatta ccgtcagccc gggcgctact ggaagagcat caactaacac
     1861 ttgtaattgt ctgggttgat agccattgtc cagtagagca tgaacacctt cataacatcg
     1921 cttatttgat acaaggccgc tcaactccaa tacccggctc gcatcctgct gtcggtcctg
     1981 gaaatacgtt cttagagcat ttatgttgga aggtgcatcc tctttaaaca gaaaggtatt
     2041 cgttgtagtc caggagccta accagtcagc aagcgagcaa aaacctgcta acagtgatga
     2101 acaatcaggt ggtatatcgt ttatagataa cccggctggc gttaaaaata atgcttcgag
     2161 tacagatatc cactcctcac gagcctgttt atcttgcgca gcataagatg ccagagaagc
     2221 tggcatttcc caacgcgact tatcttgatc ctgggaatgt aatataaaac catgatgtcc
     2281 tgtaacagcc tctacccatg gaaaccagga ctcataagga tgaggagcgg catcaaaaaa
     2341 actgaaaaaa tccccgggag attgctctga aagtgaatcc tggttaaacc aatacagacc
     2401 ggctgcacca tgattaaatt tacggcacat ttgtgttgat ggaccattaa gtgatggcgt
     2461 tgcaggattt aatttcagcc aactttctgc tgatttatat tggaatcgta tatcaaactt
     2521 tccgatatca tgaagggcaa tgaaaaataa cagccaggct ttcaccttct gttttgatag
     2581 catttcattt cggcaaaaag cattttgtag tacgactgat tgatcccacc agcaatctgc
     2641 aacagctgca acatccaggc aatgataaat caacagatga atatcatttc ctttcgtcag
     2701 gctttttgag gatttccccc agtaatgaca tatatattta aaaggttcca ttaatagcct
     2761 ccctgttttt ttagtattat cgatgatatc agtggactat ttttcgatgc tgctcataaa
     2821 tatcgaatta cagtgatata cacatgtctt gcctccctgc aaagaagaga ctaacattcg
     2881 aattgcttag gttcgaacgc tggcctcagg ttgatagaaa tatcgcctgg ggcttttgtc
     2941 catctggaac ctcgcgaatg cttaacgcca gacagcctca agcacccaac tccattctat
     3001 acctgttaat tctccctgcg atgttcacct ttaaatgccg gaatgctgct cgcaaaataa
     3061 aaacgccccc atcatttctg acagaggcgt ttaatttatc cggcaatatt tacccttcgt
     3121 gtaacccaca ttcccttttt aagccaaaga aacgcgtttc ttcttccgcc atgccgggtt
     3181 cccatttacg ggttgtatgg gtatcaccga ccgataaata tccttcgtcc cataatgggt
     3241 gatatttcag gccatgtttt tgcaggtact gataaatagt tcggttatcc cagtcgataa
     3301 tcggcagcac tttaaatacg ccacgctgaa ttgccagcac cggtaaattg gcgcggctgc
     3361 cggattgttc acggcgcagg ccagcaaacc aggtttgcgc attcagttct ttcagcgccc
     3421 ggttcatcgg ttcgactttg ttgatctcgt tgtacttctc aatcccctca acaccctgct
     3481 cccacagttt accgtagcgc gcttcctgcc aggcggcgct ttcggtagcg cggtacactt
     3541 tcaggttgag cttgagtttg tccgttaact cgtcaataaa gcggtaggtt tctgggaaca
     3601 gatagccggt atcggtgagg atcaccggaa tatccgggcg aatctgattc accagatgca
     3661 ggctcaccgc cgcctgaatg ccaaagctgg aagaaagtac atattcaccg ggcagattat
     3721 ccagcgccca ggcaacgcgg ccttcagcgt ccagtttttc cagttcggcg ttagtttccg
     3781 ccagcgccag aatgcgatct accttcggca gttcgttcag ggcgtttaga tcgagtttgg
     3841 acatagattc ctcactgttt gccttgcctg atgcgacgct cacgcgtctt atcaggccta
     3901 caagaccggg ctaatggtta atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg
     3961 atgcccgcac gcaccgtaaa gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag
     4021 cgccctatca gttcatcaag cgacgccagg atttccggct cggtgatgtt ttctttatac
     4081 atccgtggga tacgtgtccc aatgcggttg ccgccaagat gcaagttgta gcgacctggc
     4141 gctttaccca ccaggcccac ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct
     4201 gttacacgca tcacgatatg ctcatcgctg acaccatgtt tcgccattaa attatcgatg
     4261 ttgtcgataa aagacggcag gaaacgctcg gcttcggcca tcgccagcgg gcaagtcggg
     4321 aatgacacgc aggccattga gttttcacgc tgcggcgtga cggcattcat taacccgctc
     4381 tctttggcga tcttctcgat cttcgctttc tcgctttccg gcacaccggc gatgatcaga
     4441 ttctggttcg ccgtaatgcg gaaatcgcct ttgtggatct tcgcgatctc cagcaggccg
     4501 gttttcagcg gacgcccagg ataatcaagg atgcgaccat tttcgataaa cagcgtcaga
     4561 tgccagttat catcaatgcc cttaacccag ccaatacgat cgccgcgtcc ggtaaactca
     4621 tacggacgga tcggttcaaa tttgatcccc gcgcgacgct ccacttccgc tttaaacgtc
     4681 tcaaccccca cgcgctccag cgtgtatttg gttttggcat ttttacgatc ggttcggtta
     4741 ccccagtcac gctgagttgt cacgacggct tccgccactg ccaacgtatg ctccagcggc
     4801 agatagccaa actcactcgc ggtgcgggcg taggttttct tgttgccgtg ttcgatggaa
     4861 agcccaccgc ccaccaacag gttaaagccc accagcttgc cgttttcggc gattgccacg
     4921 aagttcatgt cgttggcgtg cagatcgata tcgttctgcg gcgggatcac taccgtggtt
     4981 ttgaacttac gcggcaggta ggtctgacca aggatcggct cttcatcagt agtggcgact
     5041 ttttcctgat cgagccagat ctccgcatac gcgcgggtac gaggcagcag atgttcagag
     5101 attttcttcg cccattcata tgcttccgcg tgcagttgcg actcgtaagg gttcgaggtg
     5161 cagagtacgt tacggttcat gtcgttagca gtcgccagcg catcaaggcc gaccgagtgc
     5221 agcatctggt gcaccggttt gacgttcttt ttcagaatgc cgtggaactg aaacgtctgg
     5281 cggttggtca ggcgaatgct gccatagatg gtgttttcac cggcaaattt gtctatcgcc
     5341 tgccactgtt tggtggtgat cacgccaccc ggcagacggc agcgcaacag catcgcgtgg
     5401 cgcggctcca gcttctgttc agcacgttcg gcgcggatgt cgcggtcatc ctgctgatac
     5461 atgccgtgga agcggatcag caggaagttg tcgcccttaa agccgccggt cagaccgtcg
     5521 tttaaatctt ccgcaatggt gccgcgcagg tagttgcttt caagcttcat gcgctcggcg
     5581 tctgtcagtt ttccttcgac cactaaaggc cctggatgtt tttcgctcat tagtagacat
     5641 ctcgctgata acggcgctct acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt
     5701 ccatgccacc aaattcggca atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc
     5761 gattagcgtc gccgcagaca taaatgtggg caccgtcatt gatccagcgc cacagctccg
     5821 cgccctgttc gcgcagtttg tcttgtacgt aaattttttc tttttgatcg cgcgaccagg
     5881 caagatcgat acgcgtcagc acgccctctt tgacgtagcg ctgccattcc acctggtaga
     5941 ggaaatcttc cgtaaagtgc gggttgccaa agaacagcca gtttttcccc ggcgcctcgt
     6001 cggcggcgcg ttgctgcata aaggcgcgga acggcgcgat gccggtgcct gggccaatca
     6061 taatcaccga cgtttccgga ttggctggca ggcggaagtt gtcgttatgt tcgataaaga
     6121 cgcggacttc accatcttct tccacacgat ctgcgaggaa gctggaggca ccaccggcac
     6181 gggcgcggcc ttccacgtcg taacgcacca caccaacggt aacgtgcact tcgttctcga
     6241 cttccgcctg cgaggaggcg atggaataca ggcgcggggt cagcgggcgc agcaggttaa
     6301 tcagcgcttc ggcatccagt tgcgccggag agaaacgcac catgtcaaca atcggcgtgg
     6361 tcgcggcgta atgctgtaac ttcgctttat cgcccaccag cggcagcagc gtttcgctgc
     6421 gggtaagcgt ggcgtagttc tcaacaatat tcgcggtgtt gacggtcagt tcaaagtgcc
     6481 actgtagcgc ttcgctcagg gtcagcgttt tgccgtcaac ggtgacaggt tcatcgcctt
     6541 tcagccacag cagttcgaca agttctttca ccagtgccgg atcgttctga taccagacgc
     6601 ccagcgcgtc acccggctgg taacgcaggc cagagtcacc taagtcaatt tcgatatggc
     6661 gaacgtcttt ttcagagtta cgcccggtaa ttttctggtt aacagagagg ctcgccacca
     6721 gcggcgcgtc tttgctgtac gggctggtgt ggatttcatt taccgcgcca gtagcgacgg
     6781 attgcgaagg tgccgcgaca ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc
     6841 actcgatggc agcagcctgg tattcaacgt cggcatcgac acggtcgagc aggcgttcac
     6901 cgcccagttc cgccagctta ctgtcaaaat ctttcccgga ctggcagaaa aattcataag
     6961 aactatcgcc gaggctaaac acggcaaaag cggtattttc cagcttcggc gcttttttgg
     7021 agaacaggaa cttatgcagc gcgacggctt cttccggcgg ctccccttcc ccttgcgttg
     7081 acgtcactac gatgagcagt ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt
     7141 tcaccagctt aacgttcagt tttgctgcta ataaatcatc gcgtaatgct tcagcaaccc
     7201 ggcgcgcatt gccggtttgc gaggcggaga taatagttat acccggcatt tctgcggctg
     7261 gcgctggcgt cgctgcaagc gcagcaggct gctgattgag tacgccccag aaatagccag
     7321 aaacccaggc aagctgggta ggagttaaat cggtcgtggc cgcctgaagg cgtgccagtt
     7381 gctccgggtt caacggaagc aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg
     7441 ttccagtaag caaagctgtt tctgcgccct gtcagcgccc ataaaacaga agaggaggta
     7501 aggttaacgg ggtgaacggt gtggattaaa gacgggatag cgataactaa taaccaaatc
     7561 gactaacctg ttttagcaat agtctttaac aacaaaacag attaaccaac ccaatgaaaa
     7621 acaaatgaat ttagccaatc attaagataa atcagcgatt ttgcgcaaca aagtcgtttt
     7681 agataatgcg aaaaaacagc ctttccggta ctctacggcg gttttatcgt cctgtagaga
     7741 aattatgatg tccaccacgt tatttaaaga tttcaccttc gaagccgctc accgcttacc
     7801 acacgtcccg gaagggcata aatgtggtcg cctgcacggg cattccttta tggtgcgact
     7861 ggaaattacc ggggaagtcg atccgcatac gggctggatt atcgatttcg ctgaactgaa
     7921 agcggcgttt aagccaacct acgagcgcct cgatcactat tatctcaatg atattccagg
     7981 tctggaaaac ccgaccagcg aggttttagc aaaatggatt tgggatcaag ttaaacccgt
     8041 tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgc accgcaggtt gcatctatcg
     8101 cggcgaatga taagagtgtg tcggcggtca atttccctta agtaacgcta tgttagggtg
     8161 ttgtgttctg gatatctggg gcatgacatg gaagacgact gcgacattat tattattggt
     8221 gccggtattg caggcaccgc ttgcgcgtta cgctgcgcgc gagcgggttt atccgttttg
     8281 ttactggaac gcgctgaaat ccccggcagc aaaaatcttt ccggcgggcg gttatatacc
     8341 catgcactcg cggaactcct ccctcagttt cacctgaccg cgcctcttga acgacgcatc
     8401 actcacgaaa gcctttccct gttaacgccg gatggcgcaa cgacgttttc cagcttacag
     8461 cccggcggtg aatcctggag tgtattacgt gcacggttcg atccgtggct ggttgccgaa
     8521 gccgaaaaag aaggtgtcga atgcatcccc ggtgcgacgg tggatgcgct gtatgaagaa
     8581 aacggcaggg tgtgtggtgt catttgtggt gacgatattc tccgcgcccg ttatgtggtg
     8641 ctggcagaag gtgccaacag cgtcctggct gaacgtcacg ggttagtgac tcgtcctgct
     8701 ggcgaagcga tggcgttggg gatcaaagaa gtgctgtcgc tggaaacatc cgctattgaa
     8761 gaacgttttc atctggagaa taacgaaggc gcagcgttgc tgttcagcgg cgggatctgt
     8821 gatgacttac ccggcggcgc atttctttat actaatcaac aaacgctctc gttagggatt
     8881 gtttgcccgc tctcttccct tacgcaaagt cgtgttccgg caagcgagct gctggctcgc
     8941 tttaaaacgc atccggcagt gcgcccgctt atcaaaaaca cggaatcact ggagtatggt
     9001 gcgcatctgg tgccagaagg tggcttgcac agtatgccgg tgcaatacgc cggtaacggc
     9061 tggctgctgc tgggcgatgc gttgcgcagt tgcgtcaata ccggaatttc cgtgcgcggc
     9121 atggatatgg cgctgactgg cacgcaggcg gcggcacaaa cgctgataag cgcctgccag
     9181 caccgcgagc cgcaaaatct gtttgcgctt tatcatcaca acgtagagcg cagcctgcta
     9241 tgggatgttc tacaacgtta tcagcatgtt ccggcgcttt tgcaacgccc tggatggtac
     9301 cgtgcgtggc ctgcgttaat gcaggatatt tcccgcgatt tatgggatca gggtgataaa
     9361 cctgttccac cgctgcgcca gttattctgg cgtcatttac gtcgtcacgg cctgtggcat
     9421 ctggcgggcg atgttatcag gagtctgcga tgtctgtaac ccgtaatctc tggcgcgttg
     9481 ccgatgcgcc gcacattgta ccggctgact ccgttgagcg tcagacggta caacggttga
     9541 ttaacgcttg tccggctggt cttttttcgc tcacaccgga aggtgactta cgcgttgatt
     9601 atcgcggctg cctggagtgt ggcacctgcc gtttgctgtg cgatgaaaaa acactacaac
     9661 agtggcgcta tccgccttcc ggattcggca tcacctaccg ttttggataa ataataagga
     9721 taatttatgc ccctcttaca cctgctccgc cagaatccgg tgattgctgc cgttaaagat
     9781 aatgccagcc tgcaactggc aatcgactct gaatgccagt ttatttccgt gttgtacggc
     9841 aatatctgca ccattagtca aatcgttaaa aagattaaaa atgccggaaa atatgctttt
     9901 attcatgttg atttgctgga aggcgcgtca aataaagaag tggtgattca gtttttgaaa
     9961 ctggtgactg aggcggacgg cattatcagt accaaagcct caatgttgaa agcggcaaga
    10021 gcggaaggtt ttttctgtat tcatcgcctg tttattgttg attcgatttc gtttcacaac
    10081 attgataagc aagttgcgca atcgaatccg gattgtattg agatcctgcc aggctgtatg
    10141 cctaaagtgc tgggctgggt gacggagaaa atccgccaac cgctgattgc cggcgggctg
    10201 gtgtgcgatg aagaagatgc gcgtaatgcg attaacgcgg gtgttgtggc gctttccacc
    10261 acgaataccg gggtctggac gttagcgaaa aaattgcttt gacgggatac tcgtaacgag
    10321 ttgaatttgt gtgatttgta ggctgcacgc cacatccgac attcagcgcc tgatacgacg
    10381 cttgacgcgt cttatcaggc ttacaagtcc ccagcccaga accgtaggca ggatcatccg
    10441 gcagcacgtt attggcaatc tgcgtgaatg gttgtcacca gtgcctccag caccgctttc
    10501 cagtcatcaa ccacgccaac atctgcctgt gaaaataccg ccgcgctggc gtcatgatta
    10561 atcgccacca caaacttgct attgcgtacg cccgccatta acgctgctgc gccggaagcg
    10621 ccaacaacaa tgcacacctc aggcgccagc agatgtccgg aaataccaat cactttttca
    10681 gcatcaacgc caccgttcat cactcgcgcc cgactgtagc ccacttcggc tcccagcttt
    10741 tctaccagca tagcgatttc ctggttatct gcctctccgc cctgccccac caccagcacc
    10801 cgtctggctt cagctaatgg atcgcgggta acatttttca ggttttctat gctaatcagc
    10861 cagtcgggaa gcgcaccggg gacaatgttc agttgcagca taccagaggg caacatggtg
    10921 tttatctctg ctcccgcctg acgcgccagg gaaagacata gcgggcgttt ttctgtttgc
    10981 agcgtcgccg ttagcgcatt tccccagtgg gatttgcgca cgcttacggt tggaatatct
    11041 aacgaagtca cctggcagat gcttgcacca tgtaaacgcc aggccagccg gatcgacagt
    11101 tcatcgccaa acgcgcccgg aggaaaaagg acaacctccg caggcgttcg ttgccactgt
    11161 tccaccagcg catccagcac ctgttccgca accatcggct gtggttctat ttgccaatgc
    11221 gttagtgtgc tgccgctgaa atcctgcgcc gccagccagc ccgccattgc ggcatgttcc
    11281 tgatttattg tcacaattgc gatattcatg gctgcatcct ttggcgtaaa tagtcctgcc
    11341 atagcttttg cgctttttct gctaccgttt gcccatcaat cagcgccgct ctgcgtcgtt
    11401 gctctggcct tgccagttgc aggcactgta tcgctggcgc ttccgcggca acagtttcgc
    11461 gggtaatctc cgctttcccc gctgccattc gctggcgcat tcccggtact ggcaaggcca
    11521 cttcaccaca ctgacgcacg gcaatcaccg caggcaagcg aacccggcaa cagcgcaacc
    11581 catgttcagt acgctgttcg agggtgataa acggcgcgtc gagagtaaaa cgctccacct
    11641 gggtgaaaca gggccagccc agcatttccg ccagcaaaaa tggcgtttgt ccattctgcc
    11701 cttcgctgct ttggcagccg gtaatgatca gatccagcgg attctgacgc tgccattcgg
    11761 caatatggcg agcgacaaat tccggcgcaa agcgcagatc tgccgccgtc tccagcaata
    11821 cagcgtcctc aaacccaaga gccatgagat agcgcagcca gtgcaacgcc cgttcatccc
    11881 ccatgctcaa cgcggttaaa gacatcggcg taccgttttt tctctgcgcc aacagcagcg
    11941 cggcggcagc ctgttcatcg gcaccgagta aacttcgcag aagcgaaaca tccggtccga
    12001 tattaccctg agccgccgcc tgccactctt tttccgccag cattccggca tccggttcgg
    12061 ctttaaacgc taacagaatg ttcattcgca cctcctacat ttcccgccgc caccagcggg
    12121 agtgctttgg tttctggtgc ccatagccag gtcacaacaa aaccaaccaa caacactgcc
    12181 gccaggagca ataaggtggc ttgcattccc cactgcgcca gcacccacgg cagcaggcca
    12241 gtacttaccg ccgcccccaa ccgactcatg gcggtggcaa aaccgacgcc cagcgagcga
    12301 atgtcagtag gaaaactttc cgcaggcaaa atgcccacca gattactgac tgccgaaatg
    12361 gtggtgctga agagaacaaa aagcagcagc gttaatgaac tgccggaagg caaacaggcc
    12421 atcacgacca gcgttgccgc cagcagcaaa aaacttccca gtaaaaatct gcgatgcgct
    12481 aacaagtggg tcaacactaa ccctaataac gcgccaacaa ttaacaacgc attaagcatc
    12541 aggctggcag tcagcgcatc ttccagacca atagtttggg cgatggtcgg cagccaggta
    12601 taaatcacaa accatgggat tacgaggcag acaaagaaga cgctgttaaa cgctgtgcgc
    12661 cgccagtagc gcgaagagaa caaggttttg atgtgtttat gggtcgccgt tgccacttca
    12721 tcgcccagta aaacatgggg accaaaatag cgatgcacga tagcgtgagc ttctgcaaaa
    12781 cgcccctggc gcagtagcca gcgtggcgat tctggcgttc cccagcgtaa taacgtaatc
    12841 aacaatgcgg gcagagctgc cgatgccagt agccagcgcc aggcctccgg gctttcgcta
    12901 ataaagtgat gtccggcaat acttgccagc acatagccta cggtccacac cacgctgaat
    12961 gcgcccagca aaacaccgcg atggcggcgc ggagaaaatt cagccagcaa agtgtgaccg
    13021 actgagtaat cgcctcccag accaatgccg atcaaaatgc gcagtccaat aagatgctct
    13081 ggcgtggtgg caaaaaattg taagaacgaa gcgagcgtaa tcagcaaaaa gctgaaggtg
    13141 aagatttttt gccgaccaat atggtcggag atccacccca aaaccaggct accgataaac
    13201 aggccaagta gcgccgagcc gccgatcatt cccgccataa acggcgtcag ttgcatggcg
    13261 ggtgtaagct gaataatggc gtaaccaatg acgccgagga cataaccgtc ggtcaggtgt
    13321 gcgccgaaag tgagcgcagc aatgcggcag tgaaaacggt taagtggtaa atcatccatt
    13381 cgcaccggtg aagtgttcat ctttacccct tcattgtccg gtaataagaa cggcttctcg
    13441 cctgagaagc cgaaaaggca acgcccgtca tcgcggaacg ctgcctgaca cattattttt
    13501 caatcggata gatagtaccc gtattcataa tgccgttagg atcgaactgc tttttcagcc
    13561 cttccagcaa cgcccacgcg ctgccgtgtt ccagtttgct ccagtgaacg cgatgtttac
    13621 cgataccgtg gtggtgcacc atcgaaccac cgaggcgaat ggtttcttcg cagatgattt
    13681 tgttgagcgg attgtggtac ttgtcgattt cctcttccgg cttacagtca acgacgttgt
    13741 agtcgtagac gaagtacatg ttggtgccgt tctgatagct atgagaggag tgaccgccca
    13801 gcatggtgat gtcgtcggca tgcgggaact cggtacgaat acggttaata acgctttcgt
    13861 agatttcgtg gatgcagctc cagcagccgg acacttcggt ggtaaagccc atgttgccgg
    13921 ttttgaggat ctgcacacgt tcagcggcca ctttatccgg cccccagttc aggtgattaa
    13981 accaggtttc gatcagcttg ctgtccactc gctggcattg cgggtaacgt gccacgattt
    14041 cagcaatccc ttcgcctgtc gccttcgcaa tgcgagggtt accttcagcc ataaagatca
    14101 gcacacattt accatcggca aaatgggtga agtgttgggt gccatcttca gcgtcataca
    14161 aacgagcgat cgacggacga tacccttcca ccatgatttc acgcaggatg ttgaagccgg
    14221 ttttcatgtc ttccagcaca tagccataga agagattgtt ttccggcgta aatttgaaga
    14281 ttttcactgt gacttcagtg atatagcaca atgcaccttc gttgccgatg ataatgtgac
    14341 gaatgtccgg gcctgccgcg cggcgtggta cgtttttaat acgtgtgacg gtgccatcag
    14401 ccaatactgc ttccagacca acgaccatat cttcgattgc gccgtagagt gtggagaact
    14461 gcccgatact gcgggttgct accaggccgc ccatctgcgc cagcggtttt gattgcggtg
    14521 aatgccccgt ggtgtaacct ttttcacgca acgtgttttc cagcacctcc agcggaacac
    14581 cacattgcgc cgtgacctgc atattctcaa tatcaatatt aatgatttga ttcatggcag
    14641 agccgtcgag caccaccgag ttttccacaa cggtttccag tccaccctcg gtagcggaag
    14701 caccggtacg cggcacaccg ttaattttgt gtgcattcat aaaattcagt acacgagaga
    14761 cttgttctgt ggaaccgagt tttacgaccg ctgccggaat cggcaaagta taaatgccat
    14821 gaatatccgg aaatttgcga aaacggtcaa tactgttttt ctttaatact gtttcatcgg
    14881 taattacgcg atctgcacca acaatttcct ttagctggtc gacaatcgct gcgcgagata
    14941 aagacatagt aattccttcc tgataataaa aatggaataa tcattaataa gagaaaacag
    15001 aaaccaccgt tatggattag cgcactaaat aaccgccatc gaccactaat aaatgcccgt
    15061 tgacataatt cgatgcctga cttgcgagaa acacggctgc gcccattaaa tcctgagtat
    15121 cgccccaacg gtttgccgga atatgatcaa gaacgcgctg attggtttct ggattactgc
    15181 gtgtcgccag cgtaatatct gttgcataat agccaggggc aataccattt acctgaatat
    15241 tatattgacc tagttcatca caataagctt tggtgaaccc ggcaagagca tgtttagtgg
    15301 cagaatatgc aggtgaccat tgtccaccta agtaagagaa caatgaacag atattaataa
    15361 ttttaccgct tttctgcggg atcataattt ttgccgcttc atagcttaac tcgaatgcgg
    15421 cggtcaggtt cacatcaatc atcgggtccc agtcggcacg accgaaatcc agcaccttat
    15481 tcagcttaca aataccggca ttgttaacca gaatatcaac tgttccgaaa cgctcacagc
    15541 aagcagcgat aatcttctgc ggcgcgcctt ctgcggtgat atccacctgc atgaagtcca
    15601 tctcaacacc ctgtttttca atcatttcct ttgttttgcc gttatcttta acgaagcttg
    15661 gaataaagat atttgcacca gctttggcca gcgccatggc aaatgcctgg cctaaaccgc
    15721 tattcccgcc ggtaacaatt gcggttttac ctttcaggga gaaaaaatcc attgagaacg
    15781 cattgagaga ttcgattgac atagttagct ccaatttccg taaggcaaaa aaaaagaaag
    15841 gtaacttccc ccgcgagagg gaagttacct ttctcattat ctctggtaac tggagataat
    15901 ttttatcata agggcataat cgctaatgtg atcgtaatca cagtgttata ttcgaattgt
    15961 caaataattt taaaaagcag aagcgtgatt actctcacac aacaccactg ctttccatgt
    16021 tatttcttat tgcaattact agagacctga gaaaagtaat ccacctgaaa gggcggctta
    16081 cttttctctt ttttttgtct attacaaatg ggaatacgat atgcaacaca actcatatcg
    16141 ccgttggata accctcgcga taattagttt tagcggcggc g
//
LOCUS       DAZGAP010000127        16051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGAP010000127 DAZGAP010000000
VERSION     DAZGAP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.128"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            243..1013
                     /gene="yjjP"
                     /locus_tag="ACHLAM_003505"
     CDS             243..1013
                     /gene="yjjP"
                     /locus_tag="ACHLAM_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO7854976.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1004..1477
                     /gene="yjjB"
                     /locus_tag="ACHLAM_003506"
     CDS             1004..1477
                     /gene="yjjB"
                     /locus_tag="ACHLAM_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO7854977.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1585..2124
                     /gene="dnaT"
                     /locus_tag="ACHLAM_003507"
     CDS             1585..2124
                     /gene="dnaT"
                     /locus_tag="ACHLAM_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO7854978.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2127..2864
                     /gene="dnaC"
                     /locus_tag="ACHLAM_003508"
     CDS             2127..2864
                     /gene="dnaC"
                     /locus_tag="ACHLAM_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO7854979.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2913..3407
                     /gene="yjjA"
                     /locus_tag="ACHLAM_003509"
     CDS             2913..3407
                     /gene="yjjA"
                     /locus_tag="ACHLAM_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO7854980.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3661..5952
                     /gene="opgB"
                     /locus_tag="ACHLAM_003510"
     CDS             3661..5952
                     /gene="opgB"
                     /locus_tag="ACHLAM_003510"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO7854981.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6090..7112)
                     /gene="lgoD"
                     /locus_tag="ACHLAM_003511"
     CDS             complement(6090..7112)
                     /gene="lgoD"
                     /locus_tag="ACHLAM_003511"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO7854982.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGALVAVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7251..8165
                     /gene="lgoR"
                     /locus_tag="ACHLAM_003512"
     CDS             7251..8165
                     /gene="lgoR"
                     /locus_tag="ACHLAM_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO7854983.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8380..9741
                     /gene="lgoT"
                     /locus_tag="ACHLAM_003513"
     CDS             8380..9741
                     /gene="lgoT"
                     /locus_tag="ACHLAM_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7854984.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMIGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9790..11454)
                     /gene="tsr"
                     /locus_tag="ACHLAM_003514"
     CDS             complement(9790..11454)
                     /gene="tsr"
                     /locus_tag="ACHLAM_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO7854985.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAASEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11636..12499
                     /locus_tag="ACHLAM_003515"
     CDS             11636..12499
                     /locus_tag="ACHLAM_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO7854986.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(12509..13978)
                     /locus_tag="ACHLAM_003516"
     CDS             complement(12509..13978)
                     /locus_tag="ACHLAM_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFO7854987.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(14081..14470)
                     /locus_tag="ACHLAM_003517"
     CDS             complement(14081..14470)
                     /locus_tag="ACHLAM_003517"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7854988.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            complement(14480..15922)
                     /locus_tag="ACHLAM_003518"
     CDS             complement(14480..15922)
                     /locus_tag="ACHLAM_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO7854989.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
BASE COUNT         3967 a         4266 c         3915 g         3903 t
ORIGIN      
        1 cagttatctt ttatcctgat tcgtaagggc taaacaataa attcattata gtgataatat
       61 tcatgcgata atttgtttgc gacgagagat aattcgcttt ttaacaccga gcttctcccg
      121 ataaccagga ttaatcagcg caattttgat cttaacctgc cgcaaaacaa ccgtgcttca
      181 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
      241 ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc
      301 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
      361 cgttgggaat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacta
      421 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
      481 atgtggtgac tgaagtccag cacattgtga ttctggcgga gcaccatctg ctggattaca
      541 aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag
      601 ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg
      661 gtgccgtcat caccttcttt gccagtacgg ccgcgatgta tatccgccag ctgctggcac
      721 aacgtcatct tcatccacag atcaactttt gcctcaccgc tttcgccgcc accaccattt
      781 ccggtttgct tttgcaactt cccactttca gcaatacccc caccatcgcg atggccgcca
      841 gcgttctgct gctggtgccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag
      901 gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca ctggctacct
      961 gcgtcggcgt ggtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt
     1021 tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat
     1081 ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg ttgcttggct cgataggtca
     1141 tggttcccga atgatcttaa tgaccagcgg gctgaatatt gagtggtcaa cttttatggc
     1201 ttctatgctg gtcggcacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa
     1261 agtgtttacc gtggcggctg ttattcctat gttccctggc atatcggctt ataccgcaat
     1321 gatttcggcg gtaaaaatca gccagctagg ttacagcgaa ccgttgatga ttaccctgtt
     1381 aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc
     1441 tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcactt aaagcacgcg
     1501 gatgagagac agcctcctct cctccgtgtg ttactataaa agttatctcc cttctcgttc
     1561 atcgttccat atttgagaaa cagtatgtct tccagagttt tgaccccgga cgtcgttggt
     1621 attgacgccc tggtacacga tcaccaaacc gttctggcaa aagctgaagg cggtgtggtt
     1681 gccgtatttg ctaacaatgc cccggcgttt tatgccgtta cgcctgcacg cctggctgaa
     1741 ctgctggcgc tggaagaaaa gctggcgcgt ccgggaagcg atgtcgctct ggacgatcaa
     1801 ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca tggggaaatt cgccatgtat
     1861 ccggactggc aacccgatgc cgactttatc cgcctggcgg cgctatgggg cgtggcgcta
     1921 agagagccgg tgaccaccga agaactggcc tcattcattg cctactggca ggcggaaggt
     1981 aaagtctttc accatgtgca gtggcaacaa aaactggcgc gcagcctgca aatcggtcgt
     2041 gccagcaacg gcggactgcc gaaacgagat gtgaatacgg tcagcgaacc tgacagccaa
     2101 attccaccag gattcagagg gtaacgatga aaaacgttgg cgacctgatg caacgcctgc
     2161 aaaaaatgat gcctgcccat atcaaacctg cgttcaaaac gggtgaagaa ctactggcct
     2221 ggcagaaaga acaaggagcg atccgctccg ccgctctcga acgtgaaaat cgggcgatga
     2281 aaatgcagcg cacctttaac cgctccggta ttcgtccact gcatcagaac tgctcctttg
     2341 agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt aagcaaagcg cgccagtatg
     2401 tcgaagagtt tgacggcaac atcgccagct tcatcttttc tggcaagccc ggaaccggca
     2461 aaaaccatct ggcggcggca atctgcaacg agctgctgct acgcggtaaa tccgtattga
     2521 tcatcaccgt ggccgatatt atgtcggcga tgaaagatac cttcaggaat agcggtacca
     2581 gcgaagaaca actgcttaac gatctgagca acgttgatct gctggtgatc gatgagatcg
     2641 gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa ccagatcgtc gatcgccgct
     2701 cttcttccaa acgcccaacc gggatgctga ccaacagtaa tatggaagag atgaccaagt
     2761 tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa cagtttgtgg gtgatcttca
     2821 actgggatag ctaccgtagc cgggtaacgg gtaaagagta ttaatccccg tttctggcgg
     2881 tatactgcga cacaattcca ccttgagtaa tgatgatgaa aactgttaaa catcttctgt
     2941 gctgtgccat tgccgccagc gcattaattt ccaccggggt gcatgctgcg tcctggaaag
     3001 atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa cagcacgaca caggaaggcg
     3061 gttggtcgct cgcgtcatta actaacttgc ttagcagcgg aaaccaggcc ttaagcgcag
     3121 ataacatgaa caatgccgca ggcattctgc aatactgcgc gaagcaaaag ctggcttcgg
     3181 taaccgacgc cgaaaacatc aagaatcagg tactggaaaa actgggcctg aacagtgaag
     3241 agcaaaaaga agacaccaac tatctggatg gcattcaggg tttgctgaaa accaaagatg
     3301 gtcagcaact caatctggat aatattggaa cgactccgct ggcagaaaag gtgaaaacca
     3361 aagcctgcga tctggtgtta aagcaggggc tgaacttcat ttcctgaacc gcgaaacgcc
     3421 gcgttttagc tgttacccgg ctgacgcggc tctctcctgc ccttttccct gctcctggac
     3481 ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa aagtctgttt ataaactaat
     3541 tcctaacaac atcgcatttt tttgacacta gaattgcagc aatatggcga cgccattaca
     3601 ttgtgcttct caaaaagctt aaacgttggc taaacggccc ggtttagtga ggatcgcccg
     3661 ttgtcagaac tactctcttt cgcccttttt ctcgcctctg tgctgattta cgcatggaaa
     3721 gcgggacgta acacctggtg gtttgcagcc acattaacgg tactggggct atttgtcgtt
     3781 ttaaatatca ccctgtttgc cagcgactat tttactggcg atggtattaa cgacgcggtt
     3841 ctctatacct taaccaacag cctgaccggt gctggtgtca gtaaatacat cctgcctggt
     3901 atcggcattg tgctggggct gacagcggtg ttcggtgcgc tgggctggat cctgcgccgt
     3961 cgtcgtcatc atccgcacca ttttggttac agcctgctgg cactcttact ggcgttgggt
     4021 tcagtggatg ccagcccggc atttcgtcag ataacggaac tggtgaaatc ccagtcacgc
     4081 gacggcgacc cggactttgc ggcttattat aaagagccgt cgaaaactat ccctgacccg
     4141 aaactcaacc tggtttatat ctacggcgaa agtctcgagc ggacctattt tgataacgag
     4201 gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa atgaaggcct ggatttcagc
     4261 cacacgcagc agctgccagg aacggattac acgattgcgg gcatggtggc ttctcagtgc
     4321 ggaataccgc tgtttgcccc ctttgaaggc aacgcctccg cttctgtttc cagcttcttc
     4381 ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg gttatcagaa ctatttcgtg
     4441 cagggcgcga atctgcgttt tgccggtaaa gatgtgttcc tgaagtcgca tggcttcgac
     4501 cacttatatg gctcagaaga gctgaaaagc gtggtggccg acccgcacta tcgcaacgac
     4561 tggggattct acgacgatac cgttctcgac gaagcgtgga aaaagtttga agagctttcc
     4621 cgctcaggtc agcgattctc actgtttacc ctgacagtcg atacccatca cccggatggt
     4681 tttatctcgc gtacctgtaa ccgcaaaaaa tatgattttg acggtaagcc gaatcagtca
     4741 ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt ttatcaacaa aatcaaagcg
     4801 tcaccgtggt ttaaagatac cgtcatcgtc gtctcttctg accatttagc gatgaacaac
     4861 acggcgtgga aataccttaa taaacaggat cgcaataacc tgttttttgt catccgtggc
     4921 gacaagccgc agcaagagac gctggcggtg aagcgtaaca cgatggataa cggcgcgacg
     4981 gtgctggaca ttctcggtgg cgataactat ctcggacttg gtcgtagcag tttatccggg
     5041 cagtcaatgt cggaaatctt cctcaatatc aaagagaaaa cattggcgtg gaagccggat
     5101 atcatccgcc tgtggaaatt ccctaaagag atgaaagagt tcaccatcga ccagcagaaa
     5161 aacatgattg ccttctcggg tagccatttc cgtttgccgc tactgttgcg ggtttcagac
     5221 aaacgcgtgg aaccgctgcc ggaaagtgaa tactcagcac cgctgcgttt ccagctggcc
     5281 gatttcgctc cacgcgacaa tttcgtctgg gttgaccgtt gctacaagat ggcacaactt
     5341 tgggctccgg aactggcact ctccaccgac tggtgtgtct cgcaagggca gcttggcggt
     5401 cagcaaattg tccaacatgt tgacaaaaca acatggaagg gcaaaacggc atttaaagat
     5461 acggtcatcg acatggcgcg ttacaaaggc aatgtcgata cgctgaagat tgttgataac
     5521 gatattcgct acaaagccga cagtttcatc tttaacgtcg ccggtgcgcc agaagaggtg
     5581 aaacagttta gcgggatttc ccgtccggag tcgtggggcc gctggtccaa cgctcagctg
     5641 ggcgacgaag taaaaatcga gtacaagcat ccgctgccga agaaatttga cctggtgatt
     5701 accgccaaag catacggcaa taacgccagc cgtcctattc cggtacgcgt aggcaatgaa
     5761 gaacaaactc ttgtgctggg caatgaagtg accaccacca cgctgcattt cgataaccca
     5821 accgatgccg acacgctggt aattgtgccg ccggaacctg tctcaaccaa cgaagggaat
     5881 atcctcggac actcgccgcg taagctcggg atcggcatgg tggaaattaa agtggtagaa
     5941 cgtgaagggt aattcctgac tgatgtgtag gtcgcataag gtgcgaccta cacgccttct
     6001 ccggcttaca acacactatt aagaaacaaa aaaaacagcg cccaacactt ccccggacgc
     6061 tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccctt aatcaactca
     6121 cgattgttaa tcacatcacg ctcgtaggtt tctgccagcg tggcgaacgg gtagcgatgg
     6181 gttaacatca tgtcggcagt gattttccct tccgccatca gtcgaccgac tttagcgaaa
     6241 tcttccggcg tggcgttgcg gctacccatc atcgtcgttt cttttttatg gaattctgga
     6301 tcggagaact gcaactcacc tttaaacaga ccgacaaata ccaccgtgcc gccgtgacga
     6361 atcagattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc
     6421 gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa aatcttcggc tgatgggtcc
     6481 agcacaggca actccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc
     6541 accgctacca gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccc
     6601 gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg
     6661 ctaatagcga aaggttcaat caatgctgcc gcctgcgggt caataccgtc tgcgggcaaa
     6721 atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg cacgccaatg
     6781 actgaaattt tttcacagca attggtacgc ccgcttttac acgccgggca ttgctgacag
     6841 gcaacataag ggatcacagc aacttgctga ccatttttaa gatcagcaat atttttaccc
     6901 agcccaacaa tctccccaca tatttcatgg cctaaaacgc gtggataact aaaaaatggt
     6961 tgatttccac cccaggcatg aatatcggta ccgcaaatcc cgacagactt aatttttatt
     7021 aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa ttctttcggc
     7081 tgctggcaaa ttaaaacatt catcgtagac atgattcggc tctccatttt ttgttgcctt
     7141 agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt gaaaacacac
     7201 gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat atgagtcgtt
     7261 cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg cggggtcata
     7321 tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc agccgcacta
     7381 cagtgcgtca tattctcagc cacttacgcg aatgcggcgt cttaacgcag gttggaaacg
     7441 actatgtgat tgcgcgtaaa cctgaccatg atgacggttt cgcctgtact accgcttcaa
     7501 tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gatgataaac cagcgccagt
     7561 tacgcccagg ggaaactttt tctgaactgc aactggctcg ggccgcgggt gtcagcccgg
     7621 ttgtagtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa agcgaaaagc
     7681 gcggccagtg gagcatgaag caattcgatc aatcctacgc tgagcagctg tttgagctac
     7741 gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat catgatcccc
     7801 gctggctgca ggcgaaaacc atgctcgaac gtcatcggtt actgcgtgac aacatcggca
     7861 atagctttcg catgttctcg caacttgaca gagattttca ctccttactg ctttcagctg
     7921 ccgacaatat cttttttgat caatcgcttg agatcatctc agtcatcttt cattttcact
     7981 atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat gagcatatga
     8041 ccattctcag tgctctgatt tgccgcagtg accttgatgc cacccttgcg ctccgtaacc
     8101 atttgaattc agctaaacaa tcaatgattc gatccattaa tgagaacacg cgttatgctc
     8161 attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat caaaagataa
     8221 ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat gtttttgtga
     8281 gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg taatgcacaa
     8341 ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga aaatatcacc
     8401 ctcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt gccaccagat
     8461 ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat gttgttatta
     8521 ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggttgc aaatttaacg
     8581 attcgtgaag aattgggatt aagtgctacc gaaatcggcg ctttgctctc cgtgttttca
     8641 ctcgcttacg ggattgcgca acttccttgc ggcccactat tggatcgtaa aggcccacgc
     8701 ctgatgctgg gactggggat gttcttctgg tcactgttcc aggccatgtc tggcatggtg
     8761 cacagcttta cgcagttcgt gttggtgcgt atcggtatgg ggattggtga agcgccgatg
     8821 aacccatgcg gtgtaaaagt cattaacgac tggttcaaca tcaaagagcg cggacgcccg
     8881 atgggcttct tcaacgcagc ttctactatt ggcgttgccg taagcccacc gattctggcg
     8941 gcgatgatgc tggtgatggg ctggcgtggg atgtttatta ccattggtgt gctggggatt
     9001 tttctcgcca ttggctggta tatgctctat cgcaaccgcg agcacgtaga actgactgcc
     9061 gttgaacaag cttacctcaa tgctggtagc gtcaatgccc gccgagatcc gctcagtttt
     9121 gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg gaatgatgat cggattcagt
     9181 ggcatcaact acactgcgtg gctgtatctg gcctggcttc ctggttacct gcaaacagcc
     9241 tataacctgg atttaaaaag cacagggttg atggcggcta tccctttcct gtttggggct
     9301 gccgggatgc tggtcaacgg ttacgttacc gactggctgg tcaaaggggg aatggctccg
     9361 attaaaagcc gtaagatctg cattattgcc gggatgttct gttctgccgc ctttacgctg
     9421 gtcgtaccgc aagcgacaac atccatgaca gcggttctgc tgattggcat ggcactgttt
     9481 tgtattcact ttgccggaac atcctgctgg ggcttgatcc acgtcgcagt tgcttctcgc
     9541 atgacggcgt cggtgggcag tatccagaac tttgccagct tcatctgcgc ctctttcgcg
     9601 ccgatcatta ctggttttat tgttgatacc acccactcat tccgtctggc actaatcatc
     9661 tgcggttgcg tcaccgcagc gggggcactg gcgtacatct tcctggttcg tcagccgatc
     9721 aacgacccgc gtaaagatta aactccctat gcccgataac attttgctta tcgggcattt
     9781 ttatggcgat taaaacgttt cccaattctc gccgctatct gccacggcca ttttacgcgg
     9841 cgtagctggc gtcacggttt ttactacagc cgatgtttca cgctgctgtt gctgctgttg
     9901 ctgaatccgg aacactgcca ctgcttcggt caggcgactg gcctgctctt ccagcgcggc
     9961 ggcggcagcg gcagattctt ccaccagcgc ggcgttttgt tgggttaccc ggtccatctc
    10021 agcaaccgct aagccaacct gatcgatacc acggctctgc tcatcagaag cagaagcaat
    10081 ttcgcccata atgtccgtca cgcgggtcac ggcactgaca atctccgcca ttgtttcccc
    10141 ggcgctttcg accagcgtag agccaacatc cactttcccc accgagtctt caatcaggct
    10201 tttaatttca cgagcagcct gagcgctacg ctgggccaga ttacgcactt ctcccgcaac
    10261 caccgcaaaa ccgcgccctt gctcacccgc gcgcgctgct tcaaccgccg cgttcaaagc
    10321 cagaatattg gtctggaagg caataccgtc aattacgctg ataatatcgg cgattttctg
    10381 cgaactggtg gagatatcgc gcatagtctg caccacgtta tccaccactt tgccgccgcg
    10441 ttgcgccgtt tcggaagcac ttaacgccag gtggctggcc tgacgcgcat tttcggcgtt
    10501 ctgcttcacc gttgcggtca gttgctccat gctggctgcc gtctcttcca gcgaagcggc
    10561 ctgttgctcg gtgcgcgaag agagatcgtt attgccggtg gcgatttcgc tggcaccgct
    10621 atagatggca ttggccccgt tgcgcacatc accgacggta cgcatcagct ctccctgcat
    10681 atggcgcaaa ctctctgcca gttgccccat ctcattagag ccatccacct caatcggttt
    10741 caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca ttggcgcgac
    10801 cagcgaggct ttaataccga accagacggc gaggatgacc gccagtacga cgatcatcac
    10861 gcctaccaga atccacatcg cctggctgta ggaggcattg ttatcgctga cggcgatatc
    10921 atagagccga tcgttttgct ccatgtaagc cacatactgc ttctcgaaac cgtcctgata
    10981 tccctgggtc ggttgatcaa agaactcgtt gattttgcct gcacctaaca gttggatcag
    11041 ctccgccagc gcattgtgat aaatatcgta attacgtttg atctctgacg ctgcggcggt
    11101 gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt ccgcctgttt
    11161 cagcgaaata ctggcactct gcatcagctc agcaacggtt gaaccgctac caatattatt
    11221 ctgatccatc atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc
    11281 aacccagctg ccattcagcg tggattgctg ctggcgaatg gtttgtaaaa cagtgaaatt
    11341 ttctttgtca ttctttaagg cattaaagaa cagaccgcct gatgtcagtt gtaaaaggcc
    11401 aaaaacggcc aaaaccagca gtaagctggt cacgatcttg atacgtttta acatggtttc
    11461 tctttccact agacagatac actggttgtc ggcctggtgg tggaaaactt tatgctgtgg
    11521 aaaagcgtcg gcactgcttt tgttgcagca aacttgcgtt ttttgtgagg gtcacactct
    11581 atagcggcag tttatggtac aaagtatcaa tcttcttcga accagtgata ctcctatggc
    11641 ttatagcatc gatatcatct cctgcattac cgaacggttt atggcgttaa cagccactga
    11701 aaagcgtatc gcacaattta ttcttgatga cgtctctgcc gcagtagagt tgccaattgc
    11761 tgagctggca cggttaacgc aaaccagtca ggcgtcggtg acgcgttttg cccgtgcact
    11821 ggggtgcaaa gatgtccgcg agttgaaagt caaactggcg cagtcactgg ctgtcgggca
    11881 gcggtttatt ctcgatgtac cggatcttga cggtgttcag gggatctacg aagcgattat
    11941 tagcgtgctg gagacgcatc gtcgttcgct ggatatgaat gccatcagcc aggctgtaag
    12001 ctggttaagt caggcgcggc aaattcttgc gttaggaact ggcggcggtt ccactatctg
    12061 ttcacaggag attcagtacc gtttattccg tctggggcta ccggtggtca gtcagaatga
    12121 tgccctgatg atgagaatga tgtgctcggc agtcatgccg caggatgtgg tgattgtgct
    12181 ttcgctgggc ggttatacac cggaaatcat cgaaagtgcg gcaatcgccc gtcagtatgg
    12241 cgcgcgtgtt attgccatta cgccagcaca aacaccgctg gcggaacagg tggatctggt
    12301 attgccgtta ctggttcagg aaagtgatta catctttaag cccacccctt cccgctacgc
    12361 gatgttagcc atggtcgatg tactggcaac agaactggca atggcaaata aagcgcaggc
    12421 gaaagatcgc ctgcggcgga taaaactcgt gttagacagc caccgtgggg gcgctaaccg
    12481 ccagccactt ggcgattagc attgtcggtt atgcttcttc aggtttggtg atcacgacac
    12541 tctggttttc acgctcattg gtgataatag ccaaagcttc ttcccggctg acctgacttg
    12601 ctggcgttac caacgtcacc agtacgccag cgaccaggct gcccagcact gacggaatac
    12661 acgggttacc ccagtaagcc agccagtcgg cattcatcag caccacaatt gacatcacca
    12721 taccgctcac taacgccgcc agcgcgccct gccagttaaa gcgtagccag aagcgaccga
    12781 gaatcgagca gataaacagc ccggacatca gcatcgaaat cattttggta atgtagctga
    12841 tgatgtcatt agaggtcaga gcaaagacca gcgccaggcc aataacaaag gtcaacatcc
    12901 agcgtgagta agtaatggcc ttgtcctccg gcggcattct gccggtaatc atggtgtaga
    12961 cgtcgcgcat cataatcgcc accgccgcga tggcatcgga gctggcagaa gacatggtgg
    13021 ctgaaagccc ggcaatcagc accaccagcc caagaatggc aggtaagaaa ctggtcgcga
    13081 acaagaaggc gtagttgctg ttttccagat ttgggttcat ggtgaacgcc gccatgccga
    13141 taatggcagg caaaatcgag aagaacagat ataacacccc agtaataaca aacgaacggc
    13201 gtacagaggg aacatcttta cccgagtaaa tacgctggcg ataagacggt gttgccagca
    13261 cacctacgcc aataaccagt gccagcgaaa gcgcaggcag cacgcccatt ttatcaatcg
    13321 caaacaggct catcgctttt gggtccatcg cctgctcaat cgcactccag ccgccaacgt
    13381 gaacaacggc aagaatagcc attaatataa agccaaagaa cagaattagg gcctgaatag
    13441 tatctgtcca cactaccgcc gaataaccgc cgataataac ataaatcgca aaagctaagg
    13501 cgataattaa ttttgccaca gtaagatcaa tacccgtcgc ccaggcaaga tacatacttc
    13561 cacctaatat atgcgccccc agccagccaa tagaggcaat aaatatcatc aggccgacaa
    13621 tatttttaat taaatgacta ccgccagtat aataagagag ttcttcactc atggtcataa
    13681 aacgtaattt acgtaccggt gcaaacagcc acgccaccag caatatacct acggctccac
    13741 cgacgccata aagcattccg gcccagccat tgctgtagcc aaagcccact gcccccatac
    13801 tggaacccgt gccaaccatc gtggcaacgg ttgaccccag cgtcagaaac agcggcagcg
    13861 aacgcccgcc cagcagaaaa tcttctccgc ttttctgatt acgagaaaca aaccaaccaa
    13921 gccaaatcat ggcgatggca taaataataa agccgactaa aaagatatga ctgttcataa
    13981 ttcacatcct gccagtagag agaaattaat gtaagagttc ccggagtgct gaatgtattc
    14041 agcactcaat tgcggtctgt ttttttaatt cttaaattta ttagcgggtc gcgttataac
    14101 aggtcacatc gacttcgact ttacaatcga caaccagatc agccacacaa caaatacgtg
    14161 caggcggatt ctcgccaaaa aattctctga atactttatt aaatgactgg aaatagcggg
    14221 cgtcggtcag gatcactttc acgtgaacca catccgccag gctataaccg gcttcagtca
    14281 tgatgtcgac acagttttgg atcgccagtc gtgactgttc aacgattccg ccttctacca
    14341 cttcaccgtt tttcatgggt gtttgcccgg agacgtacag ccagccgccc gcctcaaccg
    14401 ccctggcaaa aggcaaatgc tgcccgccag tgcctgttcc accttccgta ccgtaacgtt
    14461 taatgctcat attcactcct taatgtgagg tctgccggcg caggaagcgt cccgcacggc
    14521 ctgtaatatg tttatcggga ccatagctca taacgccgtt caccatcaca gcctcaatgc
    14581 catctgccgg gcgtttggga tcagaaaagc tggcaacatc acgaatggtt tgtggatcga
    14641 acaacaccag gtccgcaaaa taaccgactt tcaccagtcc acgctggggc aggcgaaaac
    14701 ggctggcaga aagcccggtc attttgtgga tagccgtggt cagtgggaat aagccttcat
    14761 cacggcaata gtggcccagc acgcgaggga aggcgcccca taaacgtgga tgcggcatgg
    14821 gatcattggg caggccgtca gagccaatca tactgaccgg ataactcaac actcggcgga
    14881 cgtcgcgctc gtccatgttg taatagatag ccccggcagg cattagcagc ttcgcggcct
    14941 ccagcatact catctgccag tcgatggcga tttgcgccag tgttttcccg gcctgctgcg
    15001 gatgtgtttg tgaccaggtg atcacaatat cgaattcgtc ggtgacttgt ttgagatcca
    15061 gcgttgaaga gctggctgag taagggtaga catcgcagcc aatttcctgg tgttgacgca
    15121 tttggtcaaa aaaggccaac gtttcttttg tccgccccca gtttttcgcc ccggcgcatt
    15181 tatggtggga aaccacgaca ggcactttgc catgacgccc aatacgaaac gcctcatcca
    15241 gggcatcgag aatcggttca aattcagaac gcagatgggt ggtataaacg cccttctcac
    15301 cggctaattc ttccgccagc gccatgactt cttcggtagt cgcctgaaat gccgttgcat
    15361 aggccagccc ggaacttaac cccagcgccc cctgactcaa cgccagacgt aagtcggcac
    15421 gcatagcggc aatttcatcc accgtcgccg ggcgaaacag gtcgtccata tggttattac
    15481 gcagcgccgt atgaccaatc agcgtgccaa cgttcagtga cggcctggct gcttccactg
    15541 cctgggcata gctttcaacg gtaggataaa taaagtgctc tgcttccccc aacagattca
    15601 tcgggtcagg aacttcacct ttcattttcg ccgacgccgc actaatcccg cagttaccga
    15661 caatcaccgt agtgatcccc tggctgatct ttggcagata ttccgggatg cgaataacgt
    15721 tgatatcgtc atgggtatga acgtcgataa acccgggtgc cagcacccgc ccttccccgt
    15781 caatgacctg ctgcgccgta acgttaagcg caggggcaat atcgacaatc cgatcacctg
    15841 ttattgccac gtcaccgcga aattcagggc cgccgctacc gtcgataacg gtcacatttt
    15901 tgattaacca gtcaacctgc atgttcgtca ctccatttct gcaatggtga caagttaacc
    15961 atttacaagg acgaaaaaca gtggaaaact acgcaaaacc cagatgattt tatgatactt
    16021 ttattcataa ataataagat aaataaacta a
//
LOCUS       DAZGAP010000128        16017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGAP010000128 DAZGAP010000000
VERSION     DAZGAP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.129"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..937
                     /locus_tag="ACHLAM_003519"
     CDS             <1..937
                     /locus_tag="ACHLAM_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7854990.1"
                     /translation="YGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLY
                     VVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNL
                     LLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRR
                     YFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPD
                     DILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVF
                     STGDTRNISLVMTEADQALREAKSEGGNKVIIHHI"
     gene            1118..2608
                     /gene="dgcJ"
                     /locus_tag="ACHLAM_003520"
     CDS             1118..2608
                     /gene="dgcJ"
                     /locus_tag="ACHLAM_003520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO7854991.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPDLRGTLQTASTDCDTIVEAAALLPAFDQAVESNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2651..3154
                     /gene="yeaK"
                     /locus_tag="ACHLAM_003521"
     CDS             2651..3154
                     /gene="yeaK"
                     /locus_tag="ACHLAM_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO7854992.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3155..3259)
                     /gene="yoaI"
                     /locus_tag="ACHLAM_003522"
     CDS             complement(3155..3259)
                     /gene="yoaI"
                     /locus_tag="ACHLAM_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO7854993.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3429..3875
                     /gene="yeaL"
                     /locus_tag="ACHLAM_003523"
     CDS             3429..3875
                     /gene="yeaL"
                     /locus_tag="ACHLAM_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO7854994.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3832..4653)
                     /gene="nimR"
                     /locus_tag="ACHLAM_003524"
     CDS             complement(3832..4653)
                     /gene="nimR"
                     /locus_tag="ACHLAM_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO7854995.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4750..5931
                     /gene="nimT"
                     /locus_tag="ACHLAM_003525"
     CDS             4750..5931
                     /gene="nimT"
                     /locus_tag="ACHLAM_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO7854996.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVAAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            5986..6333
                     /gene="yeaO"
                     /locus_tag="ACHLAM_003526"
     CDS             5986..6333
                     /gene="yeaO"
                     /locus_tag="ACHLAM_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO7854997.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6355..6609)
                     /gene="yoaF"
                     /locus_tag="ACHLAM_003527"
     CDS             complement(6355..6609)
                     /gene="yoaF"
                     /locus_tag="ACHLAM_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO7854998.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6792..7817
                     /gene="dgcP"
                     /locus_tag="ACHLAM_003528"
     CDS             6792..7817
                     /gene="dgcP"
                     /locus_tag="ACHLAM_003528"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO7854999.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            7850..7948
                     /gene="yoaK"
                     /locus_tag="ACHLAM_003529"
     CDS             7850..7948
                     /gene="yoaK"
                     /locus_tag="ACHLAM_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO7855000.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            7951..8025
                     /gene="yoaJ"
                     /locus_tag="ACHLAM_003530"
     CDS             7951..8025
                     /gene="yoaJ"
                     /locus_tag="ACHLAM_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO7855001.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8084..8332)
                     /gene="yeaQ"
                     /locus_tag="ACHLAM_003531"
     CDS             complement(8084..8332)
                     /gene="yeaQ"
                     /locus_tag="ACHLAM_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7855002.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8480..8662)
                     /gene="yoaG"
                     /locus_tag="ACHLAM_003532"
     CDS             complement(8480..8662)
                     /gene="yoaG"
                     /locus_tag="ACHLAM_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO7855003.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8666..9025)
                     /gene="yeaR"
                     /locus_tag="ACHLAM_003533"
     CDS             complement(8666..9025)
                     /gene="yeaR"
                     /locus_tag="ACHLAM_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO7855004.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9198..9836)
                     /gene="leuE"
                     /locus_tag="ACHLAM_003534"
     CDS             complement(9198..9836)
                     /gene="leuE"
                     /locus_tag="ACHLAM_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO7855005.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(9963..10886)
                     /gene="dmlR"
                     /locus_tag="ACHLAM_003535"
     CDS             complement(9963..10886)
                     /gene="dmlR"
                     /locus_tag="ACHLAM_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO7855006.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            10989..12074
                     /gene="dmlA"
                     /locus_tag="ACHLAM_003536"
     CDS             10989..12074
                     /gene="dmlA"
                     /locus_tag="ACHLAM_003536"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO7855007.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVID
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12325..13935
                     /gene="yeaV"
                     /locus_tag="ACHLAM_003537"
     CDS             12325..13935
                     /gene="yeaV"
                     /locus_tag="ACHLAM_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO7855008.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHHV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            13967..15091
                     /gene="yeaW"
                     /locus_tag="ACHLAM_003538"
     CDS             13967..15091
                     /gene="yeaW"
                     /locus_tag="ACHLAM_003538"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO7855009.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15147..>16017
                     /gene="yeaX"
                     /locus_tag="ACHLAM_003539"
     CDS             15147..>16017
                     /gene="yeaX"
                     /locus_tag="ACHLAM_003539"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO7855010.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGCNQ
                     VNHLIPTRVLVKGG"
BASE COUNT         3984 a         3922 c         3869 g         4242 t
ORIGIN      
        1 ttatgggaaa gacaacatcc ttgacaacaa taagaagaaa actggcattc tgttgctggc
       61 gctgattcct tttttagttt ttccccttct ggcacacaat ctgagcagtt ataacgctga
      121 ctattctttg tatgtcgtcg attactgtcc ggacaaccat actgcgacct ggggaatcaa
      181 ctatacaaaa atattggttt gtttgtgggc atttttactg ttctttatta tcatgcgtac
      241 acgattagcc agcgaactat ggccgttaat agcattatta tgtctggcat cgctatgctg
      301 caacttactt ctactgactc tggatgagta taattacacc atctggtata tcagtcgcgg
      361 gattgaagtt tccagtaaac tgtttgttgt gtcttttctg atttataaca tttttcagga
      421 actgcaactc tccagcaaac tggcagttca tgatgtactg accaatattt ataatcggcg
      481 ctactttttc aacagcgtag agtcattatt gtcgcgacct gttgttaagg acttttgtgt
      541 catgctggtt gatattaatc agttcaaacg catcaatgcc caatggggac atcgtgtggg
      601 tgataaagtg ctggtatcaa ttgtcgatat tatccagcaa agcatccgcc ccgatgatat
      661 tttagcgcga ctggagggtg aggtgtttgg cttgctattt accgaactca atagtgccca
      721 ggcaaaaatc attgcggaac gtatgcgtaa aaatgtcgaa ctcctgaccg gctttagtaa
      781 cagatatgat gttcctgagc aaatgaccat cagtattggc acggtttttt caacgggtga
      841 cacacgtaat atctcgcttg tcatgacgga agcagatcaa gccttacgcg aagcgaaaag
      901 cgaggggggc aacaaagtga ttattcatca tatttaagtg caaaaatatt cagagccatg
      961 ctttttgcgt ggcttttgca tacaatttat tactattacc cctaaatttc ccctccccag
     1021 cggcgcagag atgagtataa ttagcgcccc tgtgccaggc cgcaatcgaa ctttatctgg
     1081 ttttctcgtt tcactaaccg aaggagtgcc atttatcatg aaattgcacc atagaatgct
     1141 ccggcatttt atcgccgcaa gtgtcattgt gctgacatct tccttcctta tttttgaact
     1201 tgtcgccagc gacagagcaa tgagtgccta tctgcgctat atcgtgcaga gagcagactc
     1261 ctcctttctt tatgataagt atcagaatca gagtattgcc gcgcatgtga tgcgcgctct
     1321 cgctgctgag cagtcggaag tgtcgccaga acagcggcgc gccatctgcg aggcttttga
     1381 gtctgccaat aatacccatg gcttaaacct gactgcccat aaatacccgg acttacgcgg
     1441 cacactacaa accgcatcca ctgactgcga cacaattgtg gaagctgcag cactattacc
     1501 cgcttttgat caggcagtgg aaagcaaccg ccaccaggat gattacggtt caggtcttgg
     1561 gatggccgaa gagaaatttc actattatct cgatctcaat gaccgctatg tctattttta
     1621 tgaaccggtt aatgttgagt actttgcgat gaataactgg tctttcctgc agtcaggaag
     1681 tattggcatc gatcgcaaag atattgaaaa ggtatttacc gggcgtaccg ttttatcgag
     1741 catttaccag gatcagcgta ctaaacagaa cgtgatgagt ttgctgacgc cggtatatgt
     1801 cgcagggcag ctaaaaggga ttgtgctgct ggatattaac aaaaacaatc tgcggaatat
     1861 cttttacact catgaccgcc ctctcctctg gcgttttctc aatgtcacgc taaccgatac
     1921 cgattcgggg cgcgacatta tcatcaacca gagcgaagat aatctgttcc agtatgtcag
     1981 ttacgtccat gacttaccgg gcggcattcg tgtctcgtta tccattgata ttctttactt
     2041 tatcacgtct tcgtggaaaa gcgttctgtt ctggattttg acggcgttaa ttttgctgaa
     2101 tatggtgcgg atgcacttcc gtttatacca aaacgtgtcg cgagaaaata ttagtgatgc
     2161 gatgactgga ctgtataatc gcaaaatttt aacccctgaa ctggagcagc ggttgcagaa
     2221 gctggtgcaa tccggttctt cggtgatgtt tattgcaatt gacatggaca agttaaagca
     2281 aataaatgac accctcggtc atcaggaggg ggatttagcg attacgttat tagctcaggc
     2341 gattaaacaa tcgattcgta aaagtgatta tgccatccga ctcggtggcg atgaattctg
     2401 catcattctt gtcgattcga cgccgcaaat tgcagcacaa ctgcctgaac gtatcgaaaa
     2461 acgtctgcaa catatcgcgc cgcagaaaga gatcggcttc tcttccggta tttacgcgat
     2521 gaaagaaaac gatacgttac atgatgcgta taaagcttcc gatgagcgtt tatatgtcaa
     2581 taagcagaac aaaaacagcc gttcatgata accttctgtg gttgtttgct tgtaatctca
     2641 ggagcatgaa atgactgaaa tggctaaagg aagcgtgacg catcagcggt taatcgcttt
     2701 attatcacaa gaaggtgctg acttccgcgt cgttacccat gaagcggtag gtaaatgtga
     2761 agcggtgtca gaaattcgtg gcaccgcttt aggccagggg gctaaggcac tggtatgtaa
     2821 agtcaaaggc aatggtgtaa atcaacatgt tctggcgatc ctcgcggccg atcaacaggc
     2881 cgatctgagc caactcgcca gccatatagg cggattacgc gcttcgttgg ccagcccggc
     2941 ggaagtcgat gaactcacag gctgtgtctt cggcgcgatc ccccctttca gcttccatcc
     3001 aaaactcaaa ctggttgccg atcccctact cttcgaacga tttgatgaaa tcgccttcaa
     3061 tgcaggcatg ctggataaat ccgttatttt gaaaaccgcg gattatttgc gcattgcgca
     3121 accagaactg gtcaatttcc gccgcactgc gtaactagcc tgtccgttcg ataagaagaa
     3181 cggacaaaac cagcacaaca gcaatggcaa aaaacgatga cgtgataatc agtgtctcga
     3241 cgaacatttg atcgttcata gtatgccccc gggagatagt tgttttcacg ttattgttag
     3301 cgtgcgcaaa tggcagtttg atgacagttc gcctgatttt ttatgcaaaa aacgtaaaga
     3361 ttttctactt ccttcctgca gcaagcgtaa agtaagcagg cttattattc tttggcaagg
     3421 aaaccacgat gtttgatgtc actttgctga tcctgctcgg gttagctgcg ctgggcttta
     3481 tcagtcataa caccactgtc gccgtttcaa ttctggtgtt aatcattgtc cgcgtcacac
     3541 cgttaagcac ctttttcccg tggattgaaa aacaggggct aagtatcggg ataatcattc
     3601 tgacgattgg cgttatggct cccatcgcca gcgggacgct accgccctca accctgattc
     3661 actcgtttct gaactggaaa tcgctggtgg caattgcagt cggcgttatt gtctcctggc
     3721 tgggggggcg cggcgtgacg ttaatgggca gccagccgca actggtcgcc gggctgctgg
     3781 tgggcactgt tttgggcgtg gcgctgtttc gcggcgtacc ggttggcccg cttattgccg
     3841 cgggtctggt ttcgctgatt gtggggaaac agtagttaat cccgcaatat atcggcctgg
     3901 cgtttgcccc aggcctttct taaacatagt aataaaggcc gttgttgaat cataccccag
     3961 cgtatgagca actttctgta cagtatcgcc tttcactaat ccctgaagcg ccataatcag
     4021 ctgtaactgc tgacgccact ggcgaaaact cacccccgtc tctttcacta tcagtcgggc
     4081 aagattgcgt tcactcatcg caaaaaagcg agcccattgc cccaatgacc cccactcgac
     4141 gggctctttc gccatcatct ccaccatcgc gcggatttta ggatgagaag aaacgggtag
     4201 gtgcagctgt tgttgtggct gctgagggag ttcatcaaac aggacctgaa ttaatctgcg
     4261 ggtcatcggc tcagcacgct gagtcgtcgt cctgtttgcc agggttaaaa ttaattcccg
     4321 gcataagggc gatattttca atgtgcaaca ggttgtcggc attgtcactg cgctgggttc
     4381 gataaacaga aaacagagtt cagcgttagc tgtcacttga ttgctatgtt ccacccctcc
     4441 cggtatccaa accgcatatt gtggcggcac catccacaaa gcattttcca ccgtacaggt
     4501 aattgcacca tgtagtgcca gtatcaactg tcctttgcgg tgctgatgta caggactggt
     4561 taactcatcc gttccggcgt gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg
     4621 ttcatagcca ttgagattca acctgtgcat cattttgtcc gaatttagcg ataatttgtc
     4681 attttagctt gattcaacat aacaataaaa acggtaaggt acagcctcgt ttgtaacaat
     4741 gagaagcata tgacctgttc aacttcatta agcggcaaaa acaggattgt ccttatcgct
     4801 ggcattctga tgattgccac aacattacgc gtcaccttta ccggcgcagc accgttactg
     4861 gatacgattc gttccgctta ctcgctgacg acggcgcaaa ccggcttatt gaccaccctg
     4921 ccattattag cctttgcgct aatctcacct ttggctgccc cggtggcgcg acgttttggt
     4981 atggaacgta gcctgtttgc cgcgttactt ttgatctgtg ctggtatcgc aattcgctct
     5041 ctcccttcgc cttacttatt atttggcggt acagcggtca ttggcggtgg gattgcatta
     5101 ggcaatgtct tactgccagg attaattaaa cgcgatttcc ctcattccgt cgccagactt
     5161 accggcgcat attccctgac aatgggagct gcagcggcac tgggatcgac tatggtcgtg
     5221 ccgctggctt tgaacggttt tggctggcaa ggcgcgttgc tcatgctgat gtcttttcct
     5281 ctgctggctc tttttttatg gctgccacag tggcgaagtc aacaacatgc aaatttgagt
     5341 acctcacgcg ccttacatac tcggggtatc tggcgttcgc cgcttgcctg gcaggtaaca
     5401 ttgtttcttg ggatcaactc actggtctat tacgtgatta ttggctggct tccggcgatt
     5461 ctcatcagtc acggctatag cgaagcacag gcgggttcac tgcatggttt gctgcaacta
     5521 gccacagcag ctcccggttt gctgatccca cttttcttac atcatgtgaa agatcagcgt
     5581 ggtattgcag cgttcgttgc cttgatgtgc gcagtggccg cggttgggct ctgctttatg
     5641 ccagcacacg cgatcacctg gactctgctt ttcggttttg gttccggcgc aacaatgata
     5701 ctggggttga cgttcattgg tctgcgggca agttctgcgc atcaggcggc ggcactctcg
     5761 gggatggcac aatccgtcgg gtatttgctg gcagcctgtg ggccgccgct gatgggtaaa
     5821 atacacgatg ctaacggtga ctggtccgta ccactgatgg gtgttgcaat actttcacta
     5881 ctgatggcga ttttcggact ttgcgccggg agagacaaag aaattcgcta atatccggtg
     5941 ctatagtgac gtggcaaatc atgcgtgaaa gggagaacaa atacgatgaa tattcagtgc
     6001 aaacgcgttt atgatccggc tgaacagagc gatggttatc gcgtactggt cgaccgcctc
     6061 tggccacgcg gtatcaaaaa aaccgattta gcccttgatg aatgggataa agaaatcacg
     6121 ccgtcaacgg aactgcgcaa agcctttcac ggcgaagtcg tcgattttgc aacctttcgc
     6181 gagcaatatc ttgcagaact ggcgcaacac gagcaagaag gaaagcggct ggcgaacatc
     6241 gccaaaaaac agccgctgac cctgctctac tcagcaaaaa acaccacgca gaaccatgcg
     6301 ctggtgctgg cagactggct acgtagcttg tgattttagt acgccatccg gcagttattt
     6361 ttcaccagcc ggatggtccc gtcgccacaa tgcccattca tcaatcgttt caccgcccgg
     6421 taatttgcaa ttgttgctga ccccttgcgc tgtctgcaca ggaatgagcg tcccgccctt
     6481 ctgctggcaa tagaccgacg ccggatttgc cataccaatc tgcggcggtt taggtgcttc
     6541 tggctgagaa ggggttgaac aaccagccag gaccagcaag caaggcagaa caaaactgat
     6601 aattttcatt tattgatctc acatatttat ccagacttag agtatcgcgg tatcgttttg
     6661 ttttgcagca ctatttttat tacattcact caaatcatat tacgccttgt ttcatctttg
     6721 ttgatgatgt tttatcatgc ctgcaaagat taaataatca gcatttaccc gccgtatcct
     6781 ggagttgttt cgtgtcagat cagattatcg cccgcgtctc gcaatccctt gccaaagaac
     6841 agtcactgga aagcctggtc cgacagcttc tggagatgct ggaaatggtc actgatatgg
     6901 aatcaaccta cctgaccaaa gtggatgtcg aagcgcgcct gcagcatata atgtttgccc
     6961 gtaacagcca gaaaatgcac atcccggaga attttaccgt ctcgtgggat tactcgttat
     7021 gcaaacgcgc cattgatgaa aactgctttt tcagcgatga agtccctgac cgttggggcg
     7081 actgtattgc ggcacgcaat cttggcatca ccacatttct gagcacgcca attcacttac
     7141 cggatggatc attctatggc acgctttgcg ccgccagcag tgagaagcgc cagtggagtg
     7201 aacgcgcgga acaggtttta cagttattcg ccggactgat tgcacaatat attcaaaaag
     7261 aggcactggt tgagcagctg cgcgaagcca atgctgcgct gattgcgcaa tcgtataccg
     7321 actcgttaac cgggctaccg aatcggcggg cgatttttga aaatctgacg acgctgtttt
     7381 ctctcgcccg acatcttaac cataagataa tgatcgcgtt tatcgatctg gataacttca
     7441 aattaatcaa cgatcgtttt ggtcataata gtggtgattt gttcctcatt caggttggcg
     7501 agcgccttaa tacgctccag caaaatggcg aagttattgg tcgtctcggc ggtgatgagt
     7561 ttttggttgt ttcactgaac aacgagaatg cggatatttc gtctctgcga gaacgtattc
     7621 aacagcaaat acgtggagaa tatcacttag gtgacgttga tttgtattat cccggtgcca
     7681 gtcttggcat agtagaagtc gatcctgaaa cgaccgatgc agacagtgcc ctgcatgctg
     7741 ccgatatcgc gatgtatcag gagaaaaaac acaaacagaa aacacctttt gtcgcgcatc
     7801 cagcgctaca ttcctgaggc gtattcacat ccttttgatt ggtgataaca tgcgaatcgg
     7861 tattattttt ccggttgtaa tctttattac agcggtcgta tttttagcat ggttttttat
     7921 tggcggctat gctgccccgg gagcataaag atgaaaaaaa caacgattat tatgatgggt
     7981 gtggcgatta ttgtcgtact cggcactgag ctgggatggt ggtaacgtca catctagaaa
     8041 atagcaaagg ctgcctgtgt gcagcctttg tgcaatttac gcgttaactt ttaatctttc
     8101 tgtagataaa cagcacgaca atcgcgccaa taacggcaac cacgaagctg ccaaaattga
     8161 agccatcgac tttaccaaag ccaaacagcg tgctgatcca tccgccgact acggcaccga
     8221 ctatccccag caggatagtc ataaagaatc caccaccatc tttacctggc atgatccact
     8281 tcgccagaat accggcaata agcccaaaaa taatccatga cagaatgccc attgtttcct
     8341 cacttatctg ttttgcatta gcgggttagt cgctgataaa aagcatagca caacatcgtg
     8401 agggcaagat ttgtgacgag catcacggag gatttttttg cgatggcgca gaaattgcgc
     8461 catcaacgat cagtgataat taccaaccac aaacatcatg ttcgttttcc gtgtcataag
     8521 aacgcacggt attcaccaga tcttttatca cttcagccgc cacttctggc accagcaaag
     8581 tcatcggcgt ctctgtttca taatcgacag aaacgccatt gctgttattg gtgacggtca
     8641 cggtatacgt tgctttgccc atgattcatt tcccgttatg aatgactttc cgttgttgcg
     8701 caccttccat caggacttca ggagcaacga agaagtcaat gttgaaataa gtgtcgtcag
     8761 tcatggcttc aatgttgtgc cacttttctg gagggaacac agcaaactgc cccgcttcga
     8821 taaggatcac ttgatcaggc tctgtactgt gttcatcagc gtagccgaga tatttgaccg
     8881 ccccatgcat aacggaaagg cgtgggtaaa cccccgggcg cgttccttta tcaagatgac
     8941 gttcgaatat tccggcaggc gcagtttgtt tattccagaa aggcgttgag cgcgtatgaa
     9001 tataattctg tgggatttgc agcatccttt tccctccttc ggtgaatgcg ctgaaaacgg
     9061 tttatttcag ccgtttcagg gtacgcctga taatttgcat tttaaatacc atttattggt
     9121 tactatttag caccgtatca gagaagaatc agggaggatt acaggtggga agcccgcgac
     9181 aacgcgggct gaatgcatca ggactggagc gtcgccagtc gggcggcgaa acccacaaac
     9241 atcaaaccaa tcagtgagtt gcccacttta gccagtttct ttttggtacg tatgtactgc
     9301 gtgacaaaag caccagaaat aatcaggaag ctcaaatagc agaaactcac cagttccagc
     9361 gtcgtcgcca gaataaagaa tgaaattccc gtatgtgggg cattaacatc gataaactgt
     9421 acgaaaaacg acacatagaa caaaatggct ttcggattag tcaggctcaa aattaacgcg
     9481 cgtttaaaaa tggcaccgta ttggggctca tcggatttgg cctcgttatt tttacccttc
     9541 agggtcgcgt agagaatttt actccccaga tagagcaaat aaaacgcacc aagataacgt
     9601 acaatgttga ataatatcgg ggtggtctta attaatgtcg ccactccagc ccatgccaga
     9661 aacatcaata ccgcatcgcc aataaataca ccgcaggccg caagataacc gcctttcata
     9721 ccgctactga cgctattttt gagtacaaac agggtatttg gccctggcac caacacaata
     9781 aaaatggccc caaccagata ggtccagtaa ttcagaaccc cgtattcagc gaacacatta
     9841 acctctttaa ttatctttcg atcatgcgcg attaaaggtg aatatgctaa ccaatctgta
     9901 gcggcttaga aaggagaaaa tcaggtttta acctgatatc aacccgataa ttgaatcatt
     9961 aatcaggcat gctccagtga aaaattcggg tagtgctcgg caaaatactg gcgtaaaaat
    10021 tctaccgtta tccgtacttt cgctgacgtc gccagccttg aaacataaac ggcccagaca
    10081 ttcgctggct ggtaatattc cggtagcact tgtactaaat gaccactggc aatgttttcg
    10141 ctaacatccc accaggagcg cagagcaatc ccctgcccat cgagacacca ctgatggaca
    10201 atttctccat gattagacga cagtgggccg gtaactttaa tcgcatggtg gccttcttta
    10261 ttgcgtaatt gccagacacc aaaaggatga tcgcgctctt taatgaccag gcacggcaat
    10321 gcagaaagat cggttaaatg tttcggcgcg ccatgttgcg caataaattc cggtgacgcg
    10381 cagagaatgc gataattggt cgccagtttg cgggcgatta aattaggggc gatgtcatcg
    10441 ccaatgcgaa tatcgagatc gacaccttca ttgaccaaat cgaccaaccg gtcttccaca
    10501 tcaaaacgta attccagttg cggatacgcc ttcgccagcg ctgataacgc cggggccacc
    10561 acctgtcgtc caaaaccaaa gctgctgata atacgcagca tcccctgtgg cacctgacgc
    10621 acgtcagaaa gttcgtccat catctgaccg acatcctgca aaatccgctg cgcccattca
    10681 taaatccgct ctccttcttc ggtaatggtg acgcggcggg tggtgcggtg taacaacacc
    10741 acgtttagcg tttgctccag caaggcgatg cgcttgctga cgaacgccgg agaaacgccc
    10801 agttcttccg ccacggcggc aaaaccagcc cggcgagcca ccagcataaa gacgcgcaaa
    10861 tcgttcagca gcggtaaatt attcatgatt cgtgttttat gtttcaccag ttacagggat
    10921 taattccttt tcagtcaatt ataggatggt gatgttgtca attttgatgg tcaggaagtg
    10981 agaacccaat gatgaaaacg atgcgtattg ctgcgatccc gggagacggg attggcaaag
    11041 aagtccttcc tgaagggatt cgcgtgttac aggctgccgc tgagcgctgg ggcttcgcct
    11101 tgagttttga gcaaatggag tgggcgagct gcgagtatta cagccatcac ggtaaaatga
    11161 tgccggacga ctggcatgag caacttagcc gtttcgacgc catctatttt ggtgccgtcg
    11221 gctggccgga caccgttccg gaccatattt cgttgtgggg ttcgctgctg aaatttcgtc
    11281 gtgaattcga ccagtacgtc aacctgcgcc cggttcgtct ctttcctggc gttccctgcc
    11341 cgctggcggg aaaacagcct ggcgacatcg atttttacgt ggtcagggaa aacaccgaag
    11401 gcgaatattc ctcgctcggc ggcagagtga atgaaggtac agagcatgaa gtcgtcattc
    11461 aggaatcggt atttactcgt cgtggtgtcg atcgtatttt gcgctatgcc tttgaacttg
    11521 cgcaaagccg cccacgtaag acgctaactt ctgccactaa atcgaacggt ttagccatca
    11581 gcatgccgta ctgggatgag cgagtggaag caatggctga gaattacccg gagatccgct
    11641 gggacaagca gcatattgac attctctgcg cacgttttgt gatgcaaccg gaacgcttcg
    11701 atgtggtagt ggcgtccaat ttgtttggcg atatcctttc cgatcttggc ccggcctgca
    11761 ccggcaccat tggcattgcc ccatccgcca acctgaatcc ggaacgcact ttcccgtcgc
    11821 tcttcgagcc tgtccacggt tccgcgccgg atatctacgg gaaaaaaatt gctaacccta
    11881 tcgccacaat ttgggccggt gcaatgatgc tcgattttct cggcaatgga gatgagcgtt
    11941 tccagcaagc gcataacggt attctggccg cgattgaaga agtgattgat cacgggccga
    12001 aaacacctga tatgaaaggc aatgccacca cgccacaggt tgccgacgcg atttgcaaaa
    12061 ttattttgcg ttaaggtcaa accagtttat ttgaaccgcg tcactgacgc ggttttttta
    12121 ttcgttcttt gcagtaaata acctgcgtca tttcaccttt tattgtttcc gtttcgtgtt
    12181 ttatggcttt ccgtattctt aattgtttaa tttatgtaac atgcaaataa tcaatttgtt
    12241 aatcacgaca tacaactcat taccctgcaa ataattacaa ctggcaaaag ccagagacgg
    12301 gcttttatgc ccaggagatg actcatgatg agcaatgtta agaaaaaaga tgtgccgctg
    12361 ataagcatca gcctggtggc cattcttttc atcgcagctg cattaagcct tttcccacaa
    12421 caatcggccg acgcggccaa cgcaatatac acttttgtta cgcgtacgtt aggttccgcc
    12481 gtacaggtat tggttttgct ggcaatggga ctggtgattt atttagccac cagtaaatac
    12541 ggcaatattc gtcttggcga aggaaaaccg gaatacagca cgctctcatg gctgtttatg
    12601 tttatttgtg ccggtttagg ttcttctacg ctttattggg gggttgctga atgggcctat
    12661 tattatcaaa cgcctggatt aaatatcgca ccgcgttcac aacaggcact cgaatttagc
    12721 gttccctact ccttcttcca ctggggcatc agcgcctggg caacttatac gctggcctca
    12781 ttaatcatgg cttatcactt tcatgtgcgg aaaaataaag ggctgagcct ttccggcatt
    12841 attgctgcta ttaccggcgt tcgcccgcaa ggcccgtggg gaaaactggt cgatttgatg
    12901 ttcctgatcg ccactgtcgg cgcactgacc atttcccttg ttgtcaccgc agcaaccttt
    12961 acccgtgggc tttccgcgct gaccggttta cccgataact ttaccgtaca ggcatttgtg
    13021 atcctgcttt ccggcggcat tttttgccta agctcgtgga ttggtatcaa caacggtttg
    13081 caacgtctaa gcaaaatggt tggctggggc gcgttcctgc tgccattact ggtgctgatt
    13141 gtcggtccaa ccgaatttat taccaacagc atcatcaatg ccatcggcct gaccacgcaa
    13201 aacttcctgc aaatgagctt attcaccgat ccgcttggcg atggttcatt tacccgcaac
    13261 tggaccgttt tctactggct gtggtggatc tcatacacgc ctggcgtagc aatgtttgtc
    13321 acccgcgttt cccgcggtcg taagattaaa gaagttatct ggggactgat cctcggcagc
    13381 accgtcggtt gctggttctt cttcggcgta atggaaagct atgccattca ccagtttatc
    13441 aatggcgtaa tcaacgtccc acaggtgctg gaaacactgg gcggcgagac agctgtgcag
    13501 caagttctga tgtcgttgcc agccggtaaa ttgttcctcg ccgcatacct gggcgtgatg
    13561 attattttcc ttgcctcgca tatggatgcg gtggcctaca ccatggcggc aaccagtacg
    13621 cgtaatctcc aggaaggtga cgatcctgac cgtgggctgc gtcttttctg gtgcgtggta
    13681 attaccctga tcccgctttc aattcttttc actggcgcgt cgctggaaac gatgaaaacc
    13741 accgtcgtac tcacagccct tccgttcctc gtcattttac tggtgaaagt cggcggattt
    13801 attcgctggc tgaaacagga ttacgccgac attccggctc atcatgttga acattatctc
    13861 ccgcagacac cggttgaagt cctggaaaaa acgccagtgc tccctgcggg aaccgtattc
    13921 aaaggcgaca actgagcgcc atcatcctaa cgataaaggt atccctatga gcaatctgag
    13981 ccctgacttt gtactacccg aaaatttttg cgctaacccg caagaggcgt ggaccattcc
    14041 tgcccgtttt tataccgatc agaacgcctt tgaacacgaa aaagagaacg tcttcgccaa
    14101 aagctggatt tgcgtcgctc acagcagcga actggcgaat gccaatgatt atgtgacgcg
    14161 tgagatcatt ggcgaaagca ttgtgctggt acgcggtcgt gataaggttt tgcgcgcgtt
    14221 ctataacgtg tgtccgcacc gtggtcatca gttgttgagc ggcgaaggga aagcgaaaaa
    14281 tgtgattacc tgcccgtatc acgcatgggc attcaaactc gatggcaacc tggcccatgc
    14341 acgtaactgc gaaaacgtcg ccaatttcga tagcgacaaa gcgcaactgg ttccggtgcg
    14401 tctggaagaa tatgccggat tcgtcttcat caacatggac cctaacgcca ccagcgtaga
    14461 agatcagtta cccggtctgg gcgcgaaagt gctggaagcc tgcccggaag tccacgatct
    14521 gaaactggcg gcccgcttta ccacccgcac gcctgccaac tggaagaaca ttgtcgataa
    14581 ctatctcgag tgctatcact gtggtccggc gcatccaggt ttctccgact ccgtacaggt
    14641 tgatcgttac tggcacacca tgcacggtaa ctggacgctg caatacggtt tcgccaaacc
    14701 gtcagaacag tctttcaaat tcgaagaggg cacggatgcg gcattccacg gtttctggct
    14761 gtggccgtgc acgatgctta acgtcacacc gatcaaaggg atgatgacgg tcatttatga
    14821 attcccggtg gattctgaaa ctaccctgca aaactacgat atttacttca ccaatgaaga
    14881 gttaaccgac gagcaaaaat cgctgattga gtggtatcgc gatgtgttcc gtccggaaga
    14941 tttacgtctg gttgaaagcg tacagaaagg gctgaaatcg cgtggctatc gtggtcaggg
    15001 gcgcatcatg gccgacagta gcggtagcgg aatttccgaa catggtatcg cccatttcca
    15061 taatctgctg gcgcaggtgt ttaaggacta atgacatcgg cggcggtatt ttccgccgct
    15121 gggctgattt ttgatggagt acagcaatgt cagactatca aatgtttgaa gtacaggtga
    15181 gtcaggtgga accgcttacc gaacaggtga aacgcttcac gctggtggca accgatggca
    15241 aaccgctgcc tgcgttcacc ggaggaagtc acatcatagt gcagatgagc gatggtgata
    15301 accagtacag caatgcgtat tcactactga gttcaccgca taacacctct agttatcaga
    15361 ttgccgttcg actggaggaa aactcgcgcg gcggttcccg ctttttgcat cagcaggtaa
    15421 aagtgggcga tcggttaacg atttcaacgc ctaataacct gtttgcgcta attccctcgg
    15481 ccagaaagca tctgtttatc gcgggcggta ttggtatcac ccctttcctg tcgcacctgg
    15541 cagagttgca atacagcgac gtcgactggc agctacatta ctgcacgcga aatccagaaa
    15601 gttgcgcatt tcgtgatgag ctagtccagc atccacaggc tgagaaagtt cacttacatc
    15661 actcatcaac cggaacacga ctggaattag cgcgattatt ggcggatatc gaacctggca
    15721 cacatgttta tacctgtggc cccgaggcgc taaatgaagc ggtaagaagt gaagctgcgc
    15781 gtctgaacat cgccgccgat acgctgcact ttgagcaatt tgctatcgaa gacaaaaccg
    15841 gcgatgcatt taccctggtg cttgcccgtt ccggaaaaga gtttgtggtg ccggaagaga
    15901 tgactatttt gcaggttatt gaaaataata aagcagcgaa agtggaatgt ttatgtcgtg
    15961 aagggtgtaa ccaggtaaat catttgatac cgacaagggt tttagtgaaa ggaggtg
//
LOCUS       DAZGAP010000129        15942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGAP010000129 DAZGAP010000000
VERSION     DAZGAP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.130"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..418)
                     /locus_tag="ACHLAM_003540"
     CDS             complement(77..418)
                     /locus_tag="ACHLAM_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7855011.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            complement(411..3653)
                     /locus_tag="ACHLAM_003541"
     CDS             complement(411..3653)
                     /locus_tag="ACHLAM_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7855012.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAITQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLREGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(3717..4130)
                     /locus_tag="ACHLAM_003542"
     CDS             complement(3717..4130)
                     /locus_tag="ACHLAM_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855013.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(4194..4403)
                     /locus_tag="ACHLAM_003543"
     CDS             complement(4194..4403)
                     /locus_tag="ACHLAM_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO7855014.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            complement(4499..4873)
                     /locus_tag="ACHLAM_003544"
     CDS             complement(4499..4873)
                     /locus_tag="ACHLAM_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO7855015.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(4888..5604)
                     /locus_tag="ACHLAM_003545"
     CDS             complement(4888..5604)
                     /locus_tag="ACHLAM_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO7855016.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(5670..6014)
                     /locus_tag="ACHLAM_003546"
     CDS             complement(5670..6014)
                     /locus_tag="ACHLAM_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO7855017.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(6011..6457)
                     /locus_tag="ACHLAM_003547"
     CDS             complement(6011..6457)
                     /locus_tag="ACHLAM_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO7855018.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VASRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(6454..6804)
                     /locus_tag="ACHLAM_003548"
     CDS             complement(6454..6804)
                     /locus_tag="ACHLAM_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7855019.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(6814..7140)
                     /locus_tag="ACHLAM_003549"
     CDS             complement(6814..7140)
                     /locus_tag="ACHLAM_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO7855020.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(7137..9721)
                     /locus_tag="ACHLAM_003550"
                     /pseudo
     CDS             complement(7137..9721)
                     /locus_tag="ACHLAM_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(9667..9888)
                     /locus_tag="ACHLAM_003551"
     CDS             complement(9667..9888)
                     /locus_tag="ACHLAM_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855021.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(9933..11870)
                     /locus_tag="ACHLAM_003552"
     CDS             complement(9933..11870)
                     /locus_tag="ACHLAM_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7855022.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(11934..13595)
                     /locus_tag="ACHLAM_003553"
     CDS             complement(11934..13595)
                     /locus_tag="ACHLAM_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7855023.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPSPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGIRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(13592..14155)
                     /locus_tag="ACHLAM_003554"
     CDS             complement(13592..14155)
                     /locus_tag="ACHLAM_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7855024.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQGETVELDEVPVYRQKKMQTEECHQEAPPAEKNSPVSLPPDDGQNLSPL
                     PGIDYMEDGLPDPVKAMGRILVENLIIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(14445..14810)
                     /locus_tag="ACHLAM_003555"
     CDS             complement(14445..14810)
                     /locus_tag="ACHLAM_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7855025.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            14852..15052
                     /locus_tag="ACHLAM_003556"
     CDS             14852..15052
                     /locus_tag="ACHLAM_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7855026.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALQHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(15184..15510)
                     /locus_tag="ACHLAM_003557"
     CDS             complement(15184..15510)
                     /locus_tag="ACHLAM_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO7855027.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(15856..>15942)
                     /locus_tag="ACHLAM_003558"
     CDS             complement(15856..>15942)
                     /locus_tag="ACHLAM_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855028.1"
                     /translation="ATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT         3431 a         5102 c         3778 g         3631 t
ORIGIN      
        1 gatttcgcac tttctgacaa gacagattaa aaataattaa caactaaaca ataaagagtg
       61 atttatagga taattctcat ctgactacct gctctatcgt gcaactgaaa tcactgtacc
      121 gggcgttatc cgtaatactc cactcccggc acaccaccct gaccgtccgg ttatgtttcg
      181 ggggcttcca caaaaaagcc cggtaaccac catgccatga cagaaacgcg gacagtgcct
      241 cccgctcagc atccgttgtc acccgaaaaa ccacctgaaa ggtcttcagt tgcgcattca
      301 gtcctgccgg acgacgttgc tgataaccgt cgccaaactt caccgtcacc accgacggtt
      361 tctccgtcac ctgcatccct tcccggggac accagtgaag ggtcttaatc tcatccactc
      421 agcattcctc cgtcacgacg catggataac atcaccgcct gtacccgctg gtcaatcagt
      481 tgcacaaggg tgcccgccgc ttccggccct atctgcccgt tagcgccgtc attctgaatg
      541 gcaatgtggt acaccgggga atacaccaga cccgcactgc cgttcatact gcccaccgca
      601 cgcacgccga gtgaaccatc cgctgcccgg gtcaggggca tgatggcttc aggtccggct
      661 tcccccatca gccctgcccc ttttgcaaag gcaaagtacg tgggcgtgtc cacaatactg
      721 ttactgtatg cactcaggtt tgccgaggta taaacgccgc cttttgcatt ggccaccgcg
      781 ccacccagcc agttgcctat actgccgaaa aatcctcctg caccggacat actgtttgcg
      841 gccatcttaa tgccgttgac aatggccgca ttcataagaa cttttgatat ttcctgcagg
      901 attgatgcag cccagctgcg ccattccact ttatttccgt tcagcatctc cgtgatgtta
      961 ttcaccatcc ctgagatacc gtctgtcgca agctgtgctg cctgtgaagc gtaatctgat
     1021 gcgctgtcca cccagttact tagcccttcc cgcagccctt tctgccagtc agcacgctgc
     1081 gcatccgatt cggcataaaa agcctcctgg tctttaaggc gttcactcag atactgcgca
     1141 ttctgcgcca gcgcctgtct gtaaaaatcc tcactgatat cccctgtctg atactgagac
     1201 tgcaggtccg catccttctg gcggtagccg tcacgaatct gctgcagctc ccgcatccgt
     1261 tcacggaccc gctcaccctg cccgtacccc agcagctcag cgtcatttga tgccctttcg
     1321 tctgcattat catttttcag agcctcctcc cgggcccgta actgctcccg gattttcttc
     1381 tggtcaatca gggcagcatt gcgcagcaac tcctgcttct gtatctccgt cagggttttc
     1441 agctctccct gcgaggtctg gtacttcagc ttcgccagtt ctgtattctt ccctgccagt
     1501 gccagttgtt ccttctgctg cttcagcaga cgggaaaaac tgtcttccgc tttttccgtc
     1561 tcagattttc cggcccggga tttgggttta ttcgcctcat tattgcgcca ggcttccatc
     1621 gaattacgga tgtaacgctg cctcgcctcc tgatacgtat cccccaccag accaaggtca
     1681 tccgccgcat accccagccg ggcacgctct ttttcttccc ctttcagccg ggacagggcc
     1741 agttgacgct ctgtgttatt cagggcgctc tgctgtttat catccagagt ggcctgtggc
     1801 aatcgcagcg gtacattcac cagtccctga cgctgttgca gcagttcatt acccagcccc
     1861 agcaggcggt tgaattccgt atgctgaccg ttcatgacca gcagtgactg atacgcttta
     1921 ttctgctctg ctgcctgctg acgaattaac acaacacggc gctcttccag tccggcaagc
     1981 acatcctgaa tggactgcgc tttttcctgc atctgtgcca gacgggactg ctcaacagca
     2041 aactgctctg ttgcctgagc aagctcttcc gttacagttt tcaccgatgt cagatggttt
     2101 atcatgaagc cgttaccggt cgtccagccc ggattagcca gcacatactg atacccggcg
     2161 attttttcct gcagggattt cacccggctg gcctgctcat caatcagccg gttctgctct
     2221 gtcagcgcct cccgtgttcg ctcttcatta tctgaggctt caggcagaga cattgatggc
     2281 gttttatgcg cgatttcatc aatcgtcagt gcatactggc gcgcagactc cctggcctgt
     2341 tcctgattct ggtacagcgt gtaccatgcg gcagctccca gcatcaccag tccgggtacg
     2401 cctccaacca gtcccagcgc accgctcatc agacgtgagc ccaccgccgt tgtactgttc
     2461 agcgcattct gggcggcggt tctggcagca atatttctgt tcaggcgttc ctgtgtgatc
     2521 gccagacggg cctctgccgc aatctgcatc tcagtcccgc gggctgccgc cacagcctgc
     2581 tgagcccggt acacggctgc ccttgcccgc gccgtggcaa tctgcgttcc ccttaactgg
     2641 gcctcagcca gtgccacttc attacgtgca gcggtcacaa gtcctgccgt ggcagacatc
     2701 gctccggaag ccagattacc aaagtaccgg gccaccccga cagcaaccag cacacccgca
     2761 gctgttgcca cggtatcaat atttccggcc acaccgttca gtgcgccgga gagcgttttc
     2821 gtcgctccgc tggcctcatt cgcgccgccc acccaggcca taaaggcgtt ttccaccttc
     2881 gtgatcccgt cagacaccgt ttccggcatg gctgcatatt catcacgcaa tatccccagc
     2941 tggctgatta acgcgggaac gactttatcc gccgtcagtt gaccatcgtc cgccatcgcc
     3001 ttcagatcct tacgggccac gcccatgcct gcagccagtg cgcgaatgat ccggtcccca
     3061 ctttcattga ccgaattaaa ttcctcgccg cgcaacaccc cctgtgccag cgcctggctg
     3121 aactgggtga tcaccgaacc cgcctctgcc gcactggcac cggaaatttt cagccctgtc
     3181 gaaatggcct ccgtcacctt cagcacatca tcagcactgt aaccatattc acgcatcgag
     3241 gcagccgaac gggcaaacag ggccgcatta tccgaaaatg cggtgcctgt ccgctggctg
     3301 atatccatca gcactttctg tgatgacgaa aattcatccg atgactgcga cgcctgtttc
     3361 agacgggcat tcacggagct ccattcatcc gccagcgaaa tcaggtgtcc ggtggcaaag
     3421 gcaccagcaa acgcaccggt cattccgaca gccgaagcgc ggatttccgt caactggctg
     3481 ttcagctcag ccagagcccg gcgctgctcc ctggctgccg cagcagcctg acgtccgcca
     3541 ttctgcaggg tccggtaata ttcactgccc atacgggacg cccgctggat ctccgactgg
     3601 aatgactgtg aatttgccga aattttgata atcagttcac gtaacgtcgc cattcacctt
     3661 tctccgggcg aaaaaaaacc tgccgcagca ggttttattc atcagcagat tattgattaa
     3721 gttttgggta ccagcccggg attcagactt tccggactca gtgtatacat accgccattg
     3781 aatgggtcca caagcaacca gccaacaata ccaccaaaga caaagttacc accaatgtac
     3841 catccattag ccgatgcctt gatcggcagc gttacgggct gataattctc tttactgaat
     3901 gtgatctgat aactcttttt cccgaagtaa ctgccgtcag atttttcaag tgttacattc
     3961 tgcggtgttt tgcccgaaga aattgttttc cctgactcat ctttaataat aaaatccgca
     4021 ccagacggat tactgtttac ctgtacagtc tgcgttttat ctccgacaat ggttgcacac
     4081 cccgtcagaa aaaagctgac agagaaaatc aacagcatga atttcttcat aaaatggctc
     4141 cgttcagtta attttccccg aaaatactgt aataaagatc tgcaaagtta aaattaattt
     4201 tccgaagcaa ataacagagc atcctccaga gcggccaaag gatctgcctc cggcttatcc
     4261 tcatcctcgc cccagcagag catggcgtcc ttcagtgcaa cattcatccc ctgtgccccg
     4321 aaaaccgctt tcacgatctg tgcattacgg atatccccgc gctcatcacc cagcggggat
     4381 accctgtcga actccatcca catcatcgcc tcgctcgcac tcaggctgtg gcgcagttcg
     4441 gataaggtgc gccccagacg gagcgcaagt cgcatcagaa agcgaatttc cgggcgggct
     4501 acttttttct ggccgactct gcatcagcga tcagttccag tgcctgacgc agcaagcggg
     4561 catgtaccgg accatagacg gccagcacct gctcacggtc gtccggagtg aacacccgct
     4621 gcaggtccgt atcacacagg acatcgcaga acagcgtcac atccgcttcc aggttacggc
     4681 gggttttcgc caccaccgac agggtatcgt catcctctcc atcaccattg agcacgtcct
     4741 gccacagata ccaggcctct gccgaaggct cccgcagcac cacgctgaca ttaccccatt
     4801 ccggcacctt caccgtttta tgacgaaacc ctgacagtct ggccagcgcc agcgttttca
     4861 gatccttttt catgatgacc catcccctta tccggcggct gcgctcactg tcactgtaca
     4921 ttcaacagac gtcacactct gtgctttctc tgccgaatca gtcaccacgc aggtatattt
     4981 ccccgcatca gcggactgcg cacctggctt actgaaggtg tctgtcgtct gcccgtcaac
     5041 cggctgacca tccttcttcc aggcgtattt atacggcggc gttcccccgt tggcactgac
     5101 tgacattgtc agcagcgcac cggtattcac ggtaagtgtc ttatccagat ttttcacaaa
     5161 cgccagcggt accacaaagg acaccggttt gcctttcaga cgcagtgaga acgttgcagc
     5221 caccacgccg ttggtaccgg atgaccaggt gtgctggcgc acttccgcca ggaatttaaa
     5281 gcccttaccg gacggaaact gcaccttaaa cgcatacaac gcgtcattgt cataagcatc
     5341 acgcagggcg ttctgggcct gattcagata aaaattaccc gacatggaaa tctcggacga
     5401 cgcccccaga ccattgatat tctcctgctc tgtggagcag agcgtggtca catcaatatc
     5461 ctgtttctga ccggcggtga actggacttc cttgatggtg cagtccaggc gcagatattc
     5521 cgccttatcc atagtttcag cagtcgccgg ggcagatgaa atcatcacct gcgtcagctg
     5581 tgagcgttca tacaaagcag acattctgcc tcctgataat aaaaaacccg cacgcggcgg
     5641 ggtatgggtt ttgtagaaaa aagaaaaagt cacaccgtga cctgaaactc cagggttgca
     5701 cggtaacagc ggttttccgg aatatagtcc tgcatttcac tgacggatcc cggggccagc
     5761 agcattatgg cttcacgggc gtcctgacgt atctgacgcg cctgcgtcac agtcccggca
     5821 taaacgtcta tctgcaccgc cactgaggac tccgcctgcc cgcccatcac gtccgcagac
     5881 accgatgaaa tcaggctgaa aaccacccac ggaagcgcca ccgacggcct gccatccagc
     5941 agggggacca catacgggta cacctgcccg ccggcaagat gcgccagatg aggatacaaa
     6001 tccgcctccg tcatcgtctc agtacctcat caatggcccg gttcatccga gcaatcgcca
     6061 cctgcgctgc ctgttcactg cgcacatcaa acgccgggcg cacaaacggg tgcggtggca
     6121 tattcacggt ccccatttcc acaaaccgcc agtagaaagc attgcgcggg ttatccgcct
     6181 tcatggtgtt atcgctgtta ccggtgtccg gattaacacc acggatatgg acaccggatt
     6241 ccatcccgcc atcgcgggag cgccgggaaa ggaccaccac attgcggcgc agttttcccc
     6301 tgcgtaccgg tgcccgtgac gccacttctt ctttcagcac attcgcaccc gcacgggttg
     6361 cctcacgcag cacccggtta ttttccgcac cactcagaag ctgcaaatcg cggctgatct
     6421 cctccagccc cgaaaaatcc agcagggttt cgatcatttt tcccctccca gccgacagag
     6481 aatttccaga cgcccgccgg tcgcatccgg cacgggcagc ccgacaacgt tcaggatccg
     6541 gtcacgccat ggaccactca gcacatgaag tcgtgacgct gccgtgattt cccgaccgga
     6601 ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt tccgcaccgg actgcatccg
     6661 ctcccggctg ctcctgccac ggatatccgc atgaattttc ccgcatgaca cccattcttc
     6721 cgtcatttct ccggcagcat tacgggttaa caccgggttc agaacactta tcatctgtgt
     6781 cagacgacct gcagatattg ccattccccc tcctcataac accgtcggac aacgcaaatc
     6841 gtaaatcagc acggaaacag aaaacggcag ctccccctga agcagttctt cccgctccgc
     6901 aagatccgga ttccggtaca gcatcccggt cagtcgcatg gcagccccct tcatccgggt
     6961 taatgcctcg cccgggatca gttcaccgtc ctcacgaatc accttatccc ggctgccctg
     7021 aatgtaggcc agcagcacgg cggtagcctg acgaaccttg tccatcagca tgtcatcatc
     7081 cgcgtcatgg tcgacacgca gatgtgcctt gatctcttcc agtgtcagta atgccgtcat
     7141 tttccgcctc cagcatcccg cccacgtttt gcagccaggg tccagcctga tgaatgagct
     7201 tctccgggtt tatcaccggt catactgttg cagtgccaca gcgagccccc ccacgtcacc
     7261 gtatcgccgg ggtggtaggt ttcaccggct ctgaacacac cgcggtagag catcaccggc
     7321 agggaaaatg ttttttccgt acgctggcca ctgctctgcc ggaccaccac agagaacaac
     7381 cgttcacccg tcatgctgac gtcaatatcc gccaccccgt caaccaggca ttcccatccc
     7441 cgcatcccgt gcgttttttc atacgcccgc cagagtccgc cctggtgtgt ggcatacgtg
     7501 ccccggggaa aggatttttg atcgtcaatg gcagggagta tttccagtgc cgtggcatca
     7561 cgcccgtcct gcggagccgg cagggcactc accgcatcat tcaccgcctt ctgcagaaca
     7621 tccggatcat agtcacgacc atcacgcgga acaggaatat ggcttaccgc ctccttcacc
     7681 atctgctcaa gcatcggacg cacatcatcc ggggtgatac ttttaccgtc tgccggctgc
     7741 ggaatatttg cgaccgcatc attcaccgcc ttctgcagta cttccggatc atagtcacga
     7801 ccatcacgcg gaacaggaat atggcttacc gcctccttca ccatctgctc aagcatcgga
     7861 cgcacatcat ccggggtgag gcttttaccg tctgccagct gcggaatatt tgcgaccgca
     7921 tcattcaccg ccttctgcag tacttccgga tcgtagtcac gaccatcacg cggaacagga
     7981 atatggctca ctgcctcttt caccatctgt tcaagcatcg gacgcacatc atccggggtg
     8041 atacttttac cgtccgccgg ctgcggaata tttgcgaccg catcattcac cgccttctgc
     8101 agtacttccg gatcgtagtc acgaccatct cgcggaacag ggatatggct taccgcctcc
     8161 ttcaccatct gctcaagcat cggacgcaca tcatcgcccg tcacgcactt ctgtaatacc
     8221 acagacaggg aagccagttt ttcttcaaac gtttgtgcct gcgcggctat tttctcctca
     8281 aatgtgcgct gtaaatccgc cagcaccgcg gaaaattctt cacccagcgc acggataatg
     8341 gacagttcac gttcattcat tttttcagaa tccccctgaa catcgccttc accgcgtcat
     8401 gctctgtttc agtgattgcc ttattaccgt cagatgcagc gaccgcctga ggcacggaag
     8461 ctgttttacc ggacgacgcg aacggatctt cacgggcatc acggcgggac aacgcttcca
     8521 gactgtagtt ctgctgctga agatacagtg catcgcctcc tgccaggggc ggcaggttct
     8581 cccgtttacg ggcttcattg ggcgtgagaa gcgtattttt caccgcatcc cccagcgttt
     8641 tcatgcgccg ctcactgtcc attctcagca gcgtggtgac atcaaattcc gtactctcgt
     8701 tttcccccgt ttccagcgcc tcatccagta acagctcaat ggactcaatc agtgtctgca
     8761 ggcactggga ataatactgc tgctccagcg cctccacgtt gtcactggaa ggcggttgtc
     8821 ccacgccaat cttgtaggcc gggacacgga acaccgaaca gacaatttca gcggtcatct
     8881 tcagttgttc caccgtctgc gcatccaccg gtgaaaacgt cgtggggttg tattttgccc
     8941 cgttgctcag aatggccgtt ttccccgcat tttcgcctgt atacccgctg tcccagttgc
     9001 tcttcagttt tttcgcattt tcttccgtaa tactgccggg gatctcaatc accccggatg
     9061 gcctgccgcc atttctgaaa aaatacgtcg aattttcctg aatatgatgc ccctgcgtgg
     9121 ccgccagccc agcggcatac accggcggca accctataag cggatgaaaa aaacagttaa
     9181 accggtcgtg gatcacttcc cgggcaggca ccgtcaccgc ctcagtgatc ccacagttcc
     9241 ggtccggcgt gatgcggtag aacacctcgc cgtcatccgc caccagaggt tcaacccggt
     9301 tccagtccag aatacgcagt tctttgatct gcccccgggc atcacggatt ttcagcacca
     9361 ccgtattgcc gtgacgcagt ttggcgttca gccacagttc aaaaaactgg atgcggttct
     9421 gctgggcgtt gggacgacga cagaggcggg caatatctcc ccggcgcgtt tccctgcgta
     9481 tcccctgcgc atccgtctgc ataagacgca gccgcatttt ggcgatatcc tgggatatca
     9541 gcgaaataca tgcaaacacc gcatgaaagg agaggacggc ttcaggatcg gctttcacgc
     9601 cctgctgcca ggcgccggaa aagggctcag ccaccgcctg aaacaggctg gtccagcccg
     9661 cctctcttac gtcacgtcct gatttctggt tttttcgggt tcgccgtaaa aggttccaca
     9721 ttcgccatgc tccgcatcac gtttcttttt ctgacctgcc ggacgtcgca ccgtgatgta
     9781 ctctgccttt cccaggcgaa ccagcacctc cgcacacggc tgtgccacat cccggatatc
     9841 cccggcccgg gcatcatgcg tgccctgcag gtatcggatc tttgccataa cctgttacgg
     9901 gaggcgcacg cctcccgtcc tccttatcag actcagccgc cggacgcact gccgtagttc
     9961 actccggtga tcaccgccac cgccgcggta cggcgacgac gccagttgat ccagcgctcc
    10021 gcacggatgg ccacactgcc tgtctggaac atggaaacca gctccaccgg cgacggcgtg
    10081 gtactgtcgc cggtcggctc agactgcatt tccagtgatg cctcgcggga catatccact
    10141 gccacgccgc cgtcatccgc cagataaata tccggggcat tcaccagcac cagctggtca
    10201 cccacgtact gggagacaat caccggcagc ccctggaagg agccacccag tagggtcatg
    10261 tccgggtatt ctttctgacc cagcgcattt ttacgcatgg acagtgccag ggcattggtg
    10321 ctggacatca gccagaccgc accggtgggt tgcaggtttg ctgccacaaa ctgtccaaac
    10381 gcagcctctg catccgcatc cgggttaccg cttgatgccg tgcccttcac atcatgggtg
    10441 atggacgccg gggagacatc tgccaccgcc gcttttttcg ggtccacaaa gtctgtatcc
    10501 agacgcgcca ccaccgcttc cgccagcgca ttacggacca gtgcatcagc agccggactg
    10561 gaaaaacgga tcaattcttc cgtcagtacc gcaatggccg acaccttcgc atgactgaag
    10621 gtgatggatt caaaatcaaa cttcgtcagg ggttttgcct taccctcacc cacccagccg
    10681 gcagcaccgc cggacacctg ggcgtgcaca cggatattga acggcacctg acgaagtgca
    10741 gggatcccgc cctgaccaaa tcgcccgata atggtctgag gacgcaggta atcaataaag
    10801 tcctgtgcgt attcctgata ttcagacagg ctgcctgccc actgcggatc cgtggtggtc
    10861 cccgcgccca ctgccgattt caggacatga tgcagacgac tgtcatccgg atactgacga
    10921 cgggccactt ccagggcttc agagcggaca cctttagccg cagccagcga tttggcaaaa
    10981 cgtgcgaaac caatcccctt atccagtttc tgctccacac ggatcaccgg cgcagaagcc
    11041 accgcggcca cattcccgtt accggcctgt ttcaccggct gcgccgtggc ggccttaccg
    11101 gcttccagtt cacgcaggcg cttcaggtgc gcatccacct gacggatttc cgctgcggtg
    11161 ttgtcgtaat gctcttcctc ctccacatcc agcgtgcgcc cttcctctgc ggctttggtc
    11221 atgacctcct caagggaggc tgccagcgct gcacgcttgt tttcaaaact tttaatctgt
    11281 tcgccaatat tcattatggt cttttcctta tgaaaaacgg ttgttgactg tgccgcagcg
    11341 ccggcagaag atgcgatttt caccaccggt ttccggttgc cggacgcggc agaaaacggg
    11401 cggtcgtaag atttaatggt ccggatagtg cattccgcat tcgcgggcac ggtgacggca
    11461 gacacctcca tcagctccca gcgcagaaaa tgcactccgc ctccgtccag aaaggtgtat
    11521 tcatgaggac ggaagcccac ggacagcccc ctgaccagcc cggtcttaat ggccgcccag
    11581 acctcatcca gccgggcagc cagttgcgac ggcatatccg gtacgggttt caccagtgtt
    11641 gccgtgattt ccagcccttc gctgacccgg cgcaccgtac actgccccac cgggcgggaa
    11701 tggtcatgct gccagagaaa cgggatcgca ctgccaaact ccgcgccctc cggctccagg
    11761 atgtcaccat cccgatccgg agaaggcgtt gacgcaatcc cggtgatcac ccgctcatcc
    11821 tcactgaagg atttcaccgt cagcagggag caggcccgtt taagagtcac atcagcctcc
    11881 tgaaaataaa aaaaccgccg cagcggtcca tgatggttac agggtgaaca gggttatatg
    11941 aaaaaaaccg catattcttt ctttttgggc tccggattca gggacatcag ggataccgca
    12001 ttgaacagcg ccatcagcgg gtcaattttc ccccgtccgc tggcctgttt ggtaataagg
    12061 atggcgttac ctttaggctc cacccgggca ttgccaacgc accaggccat cagtggctga
    12121 ccaccatgca ccagcactcc ctcagccagt ttgcgctcgg tggttttgat ggccccgccc
    12181 agcttccagc cctggcttat ccccacaaca attccgtcgg ggatcccggc ttctgccagt
    12241 gaatccagaa tctgccccac acctgacggg tcaataccga tatggtccag taactcagcc
    12301 tcatgaatac gacgcacata ctccgccact tccgccgtgt catccccgac ccgacggaca
    12361 atcgtcatgt ctccactggc cacaaaatcc tgaaaccggg atgcctcact cttccgtctg
    12421 accaccgcgg tttcatgcgc ccaggcatgg ccccagccca gccattcgcg ggtttccctg
    12481 tcacgaccaa tcacgtacat tcccagcaga tcatccaggc ccccgccgtc aatccccacc
    12541 gtcaccacat cagcgcgctg caggatatcg tccaggctga cgcgcctgcc ctgctgctcc
    12601 cagaaatccg cacccgccca gcggtcagaa cgcagggcaa gaccaatttc cacattggcg
    12661 tgttttgaca tgaagccacg aaatgcctcc tcaccagcct cccgggcttt acggtactcc
    12721 cggtacagaa aagcctcatc caccgaataa ccgagattcg ggttaaccat ggcgaggttt
    12781 tccatcagca ggtgctcacc gcgctccacc atctccggcg gatgctcaaa tatcaccggc
    12841 agaaagtgcg gatcattaat tttgccgtcg cgcacatccc gggcgtactg cagtttctgt
    12901 ctgaacaccc cggcgggcgg ctcattcgac tgggtggttg tgtacaccac aaacccttcc
    12961 gggcgggagg caaggccgcc gatggcttca cgtaacatgt cctccgcttt gtactgcttg
    13021 ccaaataacc acagttcatc aatcagtgtc cccacggact tgataccgga caccgtattc
    13081 ggatcggctg ccaccacctt cagggtggtg tccgtcaccc ggtgggtgat ggtccggata
    13141 tgcgtctgta cctggcagag gtcatccaga tcatcgtcac gtctgaccat atcccgcgca
    13201 ggattaaagg cgttagccgc cacctccacg gtcggggcca gaatggtgta gcccgccgcc
    13261 tgccgccagt tcagtaacag cgccgtcatc atgatccccg cggccagcgt ggacttactg
    13321 tttttcttgg ggataaggat aaaaacttcc ttgatatggc gtataccggt ctgcgcatcg
    13381 taggagccaa acagggccgc caccaggtca aacacccacg gtgcacagga ctccccgaac
    13441 gtcgggctgc ccggtgcatc cacaatccgc agttgtttaa aaatcgccag ggcatgtgcg
    13501 gcctgctccg ggtaaatcgg agacggaata atcgacagcc cctttttcag acgctctgcc
    13561 cagtccgggc aggccgtgct ccacactggt atcatccgtt gccctcatta tcattattca
    13621 ccaccaggcg ggggggtggt ggcaccgcaa aacggttagc cgctttttta gcggcatcac
    13681 cttttgccga ttttttacca gcatcgcctt ttttgtggtg tgtgaactgc gccagtcgcc
    13741 aggccgcatc cagtgccagt ttcgggtcaa ttatcaggtt ttccaccagg atcctcccca
    13801 ttgcttttac cggatctgga agaccgtctt ccatgtaatc aataccgggc agtggtgaga
    13861 ggttttgccc gtcatcaggc ggcagtgaaa ccggggagtt cttttccgcc ggaggggctt
    13921 cctgatggca ctcctccgtc tgcatctttt tctgccggta aacaggaacc tcatccagct
    13981 ccaccgtctc gccctgctta cgggcaataa acgcgagcac ttccggatct tttgccagtt
    14041 gcgagccttt aactctggcc gtcttcgccg aataacctgc cgcaatggct gacgctgttt
    14101 tgtttttccc ggacatgagc gccagcgcaa attttcgttt ttgcgtcgtc agcacagtgc
    14161 cctcccgggg tcaaaactct ctcagccggg catgcatcag gccgttttcc cacaccgcgc
    14221 ataccccgaa tgttaactgc tgcctggtta acatttgctg aaaaagcctg ttaacatttt
    14281 ttccacgcaa caaactgaat aataaagata aaaaccgcaa aaatgcccgg gcagccagtt
    14341 aacatgttaa ctgccctgaa acgggaattt tttctctgcg tgagaggggg cgcggtgtcc
    14401 ggagcgatcg ttttttacgc cggatgatcc cccccccggt cgggttacag tccgataatg
    14461 tcgtccgctc tgccactacc tccggacacc tccggcagcg tcgggtccgg cataccaccc
    14521 gccgcttcac gagcagactt ttgtcgatgg cattcggtac agagcgtcca gagattcgtc
    14581 tcctcattac caccaccgaa ctgaagtgca attcggtgat cgagttcact gtcacagagg
    14641 tcaaccacac gaccacagag acagcactgc ccggcatccc tgagccagat atgacgcttg
    14701 agggaaacac gtgcactgcc actgacacga cgctgttcac ccttcagaat attcacccgt
    14761 cgggtattca gtgttttgat tctgctctgg agtgtacgaa gctcagccat gtaaaatccc
    14821 cgtcatatgg caatcagtaa aggaaataaa tatgtcatcg aaaaaccgga cccgcagaac
    14881 cacaacccgc aatatccgtt tccccaatca catgattgaa cagatcaaca tcgcccttca
    14941 gcataaaggg tccggtaact tttcagcgtg ggttattgaa gcctgcagga gaaggctggc
    15001 aacagatgca acgcatctgc gtccggccag catgacaaat aacgagaaat gaacgttcgg
    15061 ttacaggagc aggtacccac tgtcctccaa caatatttca tcttcatacc cggcggaaca
    15121 agacttaccc agccgggatg tacagaataa caacagagtg ataattaatt tctgatgaaa
    15181 taatcagggt gcagaaggac taaagataaa cgttttcttc acgcctttac gcggcctgtc
    15241 cttctcaaat cgccattttg ccatcgcctt tacaacctgc tcatcaaaca gatggtgcgg
    15301 ctctgaacgg ataaactcaa ttcgggtgac agtaccatca gcaccaatat caaacttcac
    15361 atcaacccgt ccctttatat aatttgccgc tgcataggcc ggatattgtg gtaatgcctt
    15421 aaccaactgt cggggcatat ctgttttatg ttgcgtacag cccataacca gagaagacaa
    15481 caaaataatt aacggaagat ttcttttcat tttcattccc ggcacagata agaataagtc
    15541 ttattctaac aatgccaccc tgtcggtcat caatcctctg cttaatggca acgacaatta
    15601 tccgacttaa atcacaaatc agacacatga cataacaggt cttgcgaggt aacacatcgt
    15661 ccggtttctt ccaccatcgc accggagcag cgaccatgag gggacaacgc cgcgctccgt
    15721 taacgcggta aaccccggtg tgtatcgttt ttgattatcc ccgcacactc gcgcagagga
    15781 gtctccctgt cgggctgcgg tctctgttaa tacgggaata cggcgacaat accgcgccat
    15841 ggataataag gtcgctcaac acactggctg taatgcagcc gataccatgc ggcatttagc
    15901 ggcattcatc gtacactcaa cggttagctc ttcattcgtg gc
//
LOCUS       DAZGAP010000130        15334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGAP010000130 DAZGAP010000000
VERSION     DAZGAP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.131"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..509)
                     /gene="curA"
                     /locus_tag="ACHLAM_003559"
     CDS             complement(<1..509)
                     /gene="curA"
                     /locus_tag="ACHLAM_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO7855029.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIG"
     gene            690..1205
                     /gene="mddA"
                     /locus_tag="ACHLAM_003560"
     CDS             690..1205
                     /gene="mddA"
                     /locus_tag="ACHLAM_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO7855030.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            1205..1654
                     /gene="ydcZ"
                     /locus_tag="ACHLAM_003561"
     CDS             1205..1654
                     /gene="ydcZ"
                     /locus_tag="ACHLAM_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7855031.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(1655..1888)
                     /gene="ydcY"
                     /locus_tag="ACHLAM_003562"
     CDS             complement(1655..1888)
                     /gene="ydcY"
                     /locus_tag="ACHLAM_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO7855032.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1974..2147)
                     /gene="ortT"
                     /locus_tag="ACHLAM_003563"
     CDS             complement(1974..2147)
                     /gene="ortT"
                     /locus_tag="ACHLAM_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO7855033.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2342..2437
                     /gene="yncL"
                     /locus_tag="ACHLAM_003564"
     CDS             2342..2437
                     /gene="yncL"
                     /locus_tag="ACHLAM_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO7855034.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2534..3958)
                     /gene="patD"
                     /locus_tag="ACHLAM_003565"
     CDS             complement(2534..3958)
                     /gene="patD"
                     /locus_tag="ACHLAM_003565"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO7855035.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3980..4774)
                     /gene="ydcV"
                     /locus_tag="ACHLAM_003566"
     CDS             complement(3980..4774)
                     /gene="ydcV"
                     /locus_tag="ACHLAM_003566"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7855036.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4764..5705)
                     /gene="ydcU"
                     /locus_tag="ACHLAM_003567"
     CDS             complement(4764..5705)
                     /gene="ydcU"
                     /locus_tag="ACHLAM_003567"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7855037.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWGNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(5706..6719)
                     /gene="ydcT"
                     /locus_tag="ACHLAM_003568"
     CDS             complement(5706..6719)
                     /gene="ydcT"
                     /locus_tag="ACHLAM_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7855038.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRYAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(6737..7882)
                     /gene="ydcS"
                     /locus_tag="ACHLAM_003569"
     CDS             complement(6737..7882)
                     /gene="ydcS"
                     /locus_tag="ACHLAM_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO7855039.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGIRDPYQLTEEQY
                     QAVLKVMRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMSWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(8127..9533)
                     /gene="ydcR"
                     /locus_tag="ACHLAM_003570"
     CDS             complement(8127..9533)
                     /gene="ydcR"
                     /locus_tag="ACHLAM_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7855040.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(9612..10028)
                     /gene="hicB"
                     /locus_tag="ACHLAM_003571"
     CDS             complement(9612..10028)
                     /gene="hicB"
                     /locus_tag="ACHLAM_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO7855041.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(10072..10248)
                     /gene="hicA"
                     /locus_tag="ACHLAM_003572"
     CDS             complement(10072..10248)
                     /gene="hicA"
                     /locus_tag="ACHLAM_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO7855042.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            10470..10700
                     /gene="yncJ"
                     /locus_tag="ACHLAM_003573"
     CDS             10470..10700
                     /gene="yncJ"
                     /locus_tag="ACHLAM_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFO7855043.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(10792..12753)
                     /gene="rlhA"
                     /locus_tag="ACHLAM_003574"
     CDS             complement(10792..12753)
                     /gene="rlhA"
                     /locus_tag="ACHLAM_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO7855044.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(12826..13362)
                     /gene="sutR"
                     /locus_tag="ACHLAM_003575"
     CDS             complement(12826..13362)
                     /gene="sutR"
                     /locus_tag="ACHLAM_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO7855045.1"
                     /translation="MENLTRFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQVMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            13415..14626
                     /gene="ydcO"
                     /locus_tag="ACHLAM_003576"
     CDS             13415..14626
                     /gene="ydcO"
                     /locus_tag="ACHLAM_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO7855046.1"
                     /translation="MLRYLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(14661..15329)
                     /gene="ydcL"
                     /locus_tag="ACHLAM_003577"
     CDS             complement(14661..15329)
                     /gene="ydcL"
                     /locus_tag="ACHLAM_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO7855047.1"
                     /translation="MRTTSIAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         3751 a         4034 c         3744 g         3805 t
ORIGIN      
        1 ccgatttgac ccaccgtcgc ccccacagga cctgtcgccg cagcaacaac caatgtttcg
       61 ccctctttgg gctgaccgat atccagcagc cccatataag cggtaaagcc tggcatcccc
      121 agcacaccca gcgaccacga tggattttgc ggatgatcgc caagtttcac cagatcatca
      181 ccactggata tgtcatagtc ctgccatcca ctgtagccca gcacccagtc gccaggctgg
      241 taatcaggat gattcgactc cacaacacgg ctcaccgtac cgccgaccat tacgccacca
      301 atatcaaccg gcggtgaata agatggctca tcgctcatac gtccacgcat atacgggtcc
      361 agtgacaaat aaactgtgcg cagtaacacc tgtccttcac ccggtgtggc gatatcatct
      421 tcttcaagac ggaaattctc cggaaccggc gcgccatgcg gacgcgaggc cagaacccaa
      481 cgtcgattac gctgcttttg ttgccccata atgctctcct gtgcttttgg catgaatcca
      541 tcaatagtag caggctcgca ggaatggttt tgtttgcagc cgtcacatcc cctctgcaac
      601 acgcaataat ttcatcgagg aatccgttgc ttcgggctgt tttcgatagt attgataacg
      661 tcatcatcat tatttaatcg aggccaaaca tgtccatccg ttttgcccgc aaagccgact
      721 gtgctgccat tgcggaaatt tataaccacg ccgtgttgta tacggcggct atctggaatg
      781 accaaacggt ggatgcagat aaccgcattg cctggtttga agcgcggacc atagcaggtt
      841 atccagtgct ggtgagcgaa gaagatggcg tagtgacggg atatgcctcg tttggcgact
      901 ggcgtagttt cgatggtttt cgccataccg tggaacattc ggtttatgtc catcccgatc
      961 atcagggcaa aggtctgggg cgtaaattgt taagccgatt gattgatgaa gcgcgggatt
     1021 gcgggaagca tgtcatggtc gccgggatcg aatcgcaaaa tcaggcgtct ttgcatctgc
     1081 accagtcgct gggatttgtc gtcaccgcgc aaatgccgca ggtgggcact aaatttgggc
     1141 gctggctgga cctgacattt atgcagttgc aactcgacga acgcagtgaa ccggacgcaa
     1201 tctgatgaat cagtcgctca cccttgcttt tttgattgcc gccgggattg gtctggtagt
     1261 ccagaacacg ctgatggtgc gcatcaccca gacctcctct accattctca tcgccatgtt
     1321 gctgaactct ttggtaggaa ttgtgctctt tgtttctatt ttatggttta aacaaggtat
     1381 ggcggggttt ggcgaactgg tgtccaacgt gcgctggtgg acacttatac ctggcttgct
     1441 gggatcgttt tttgtcttcg ccagtatcag cggatatcag aatgtgggag cggcgacaac
     1501 tattgcggta ctggtcgcca gccagctgat tggcggtctg gtgctggata tcttccgcag
     1561 ccatggcgta ccactacggg cgttgatcgg cccaatttgt ggcgcaattt tacttgtggt
     1621 tggtgcctgg ctggtggcca ggcgcacatt ttaatcagag gatggtgcca cctttggtta
     1681 actgttcgtg gcgcgcttcc atatcttctt tatgtttgcg accgtgatgg gcgatcgctg
     1741 tgcgaagacg ctgctgctgg gtatagcgtt cttcccgact taactccgca tcatcgctca
     1801 gggcgattaa taattcgttc atatgggcaa tgacgctctc ttcaatcgcg gcatccacgc
     1861 gcgcgatgac ttcatccaga tgcgacatac tttctcctta gccacttgtg aaatttatcg
     1921 attcatccaa atgatataag tctgcgtggt gccgtcaagc cagtgagtaa gatttaaaac
     1981 agtgaaaaca acagcgccac cgggaaactc atcggccatg ttgcccccac cagaaaggcg
     2041 cttaagaaac ggatgcgttt cttatcgtga gaaagaaacc aggtgataag aaatgcgatt
     2101 gctgccataa ccgcataaaa aacaagcatg tgttgataga gagacatgta cgggcgtcca
     2161 gccagatgaa taaaacgcgc gcaaatatgc gtcatttttt gacgtacatc aagtttttta
     2221 accggtttga gaaaaaaaca tcaaacagca acttgccctt atccatcttc agaatgagta
     2281 ttattgcgga ttttcttaac ccgtactata cacatagggg caatgataaa aggtggcaaa
     2341 aatgaatgtt tccagtagaa ctgtagtact gataaatgtc tttgctgctg ttggtttgtt
     2401 tactcttatc tctatgagat ttggctggtt tatttgatgt agtgattttg cacgcagcac
     2461 gtactgtcag agaaatcagt gctctgacag tacgcaagat catctcatac tcttacgatc
     2521 tgcaatatta tccttaatgt ttaaccatga cgtggcggac gacggtgtaa tcctccagcc
     2581 cataaagtga catatccttg ccgtaaccag aaagtttctg cccaccgtgc ggcatttcac
     2641 ttaccagcat gaaatgggta ttgacccagg tacaaccata ttgcagccgt gcgctgacgc
     2701 gatgcgccct gcccacatct ttcgtccata ccgaagatgc aagtccgtac tggctgtcat
     2761 tcgcccagtt taccacctgt tcttcgttgt cgaagagtgt aacactcact actggaccaa
     2821 atacctcttt ttgcacgatg gcatcgtcct gtaatgcgcc agccagcagc gtcggcgcat
     2881 agtaatagcc attacccttg cgcttttcac cgccagtgat cactttgatg tgccctgtcg
     2941 ctttcgcctc ttctactgcc ttgctgacgc gttcgagatg cgccagcgag cttaaaggtc
     3001 caagctccgt agactcgtca tccggcgcac cagattttaa cgttgccacc gcagcaccca
     3061 gtttttccac cagcgtatcg taaatgcctt tttgcgcgta gatccgacaa gccgcagtac
     3121 aatcctgtcc ggcattgtaa tagccaaatg tacgtacacc ttcgaccact gcctcaatat
     3181 ccgcatcatc aaaaacaatc actggcgctt tgccaccaag ttccatatga gtacgcttta
     3241 tggacggcgc ggtatggctg atgatgtgtt ccccagtggc gatagagccc gtcagcgaca
     3301 ccatccgcac tttgggatga ccggtcagcg gatcgcccac cgttttgcct ctgccaaaca
     3361 gtacattaat cacgccagcc gggaagatat ctttcgccaa ctctgccaac ttcaacgctg
     3421 tcagcggggt aatttctgat ggtttaagca ctacgcagtt ccctgccgcc agcgccggag
     3481 ccagtttcca cgcagccatc atcagaggat aattccacgg tgcgatagaa gccacgaccc
     3541 ccaacggatc gcgacggatc atcgaagtat gaccttcaag atattcacct gccgccagac
     3601 cattcagaca gcgcgccgca ccagcgaaaa agcgaaaaac atcgacaatc gccgggattt
     3661 catcattgaa cgcactatgc agcggtttgc cacaattacg ggactccagt tcggcaaaaa
     3721 cctgaccatt ttcttcgata acatcagcca gtttcagcag acattccgca cgcactttcg
     3781 gcgtggtttg cccccattcg gcaaatgctg catctgccgc gcgaacagca gcatcgacct
     3841 gctctgcgga tgcctcggca atctccagta aaacgtcccc cgttgccgga ttatagacag
     3901 gctgtttttc cccttcgccg ctaaccagtt ctccgttaat cagtaactta tgttgcatag
     3961 catattcctg tatcagtggt tattgaccat tgtcgccttc gcgggttagc caccaggccc
     4021 ccaggatcgg caaggttgtt accaacataa ccagcagtgc caccacgtta gttaccggta
     4081 catcacgcgg tcgcccaagc tgattgagca accacaacgg taacgttcgt tcatgacctg
     4141 ccgtaaaggt cgtaacgatg atttcatcga acgacaaggc aaacgccagc attcctcctg
     4201 ccagtaacgc cgaactgaga ttcggcaaca ctacgtagcg gaaggtttgc cagccattag
     4261 ccccaagatc cattgacgcc tcaaccagac tccacgaggt gcggcgaaaa cgggcgatga
     4321 cattgttaaa gaccaccact acacaaaaag tcgcatgacc gaccacgatg gtgaaaaatc
     4381 ccggctccag attgatggtt ttaaaggcgg ttaataacgc cagaccagtg acaatgcccg
     4441 gcagcgcaat gggcagcagc agtaacagcg aaatggcgtt tttgccgaaa aagtctcgtc
     4501 gccacagcgc ggcagcggct agcgtcccta acaccagcgc gattaatgtc gccagcgccg
     4561 ccactttaag tgacagtgtc acggcatcaa gaatatcact acgctgtgct gccacgctaa
     4621 accagcgcag cgtcaggcct tgcggtggaa aactaaacgc cgcatcttca gtgttaaacg
     4681 cataaacggc gataatcaga atgggaaaat gtaagaaaac cacgccgccc caggccgcca
     4741 gtttgaggaa aaacggtgcg cgttcagagt gcatcgaacg ctcccagacg tttcacgaac
     4801 gctaggtaaa gtgcgatgag tacaatcggc actagggtga atgccgccgc catcggcata
     4861 ttgccaatcg ccccctgctg ggaataaacc atattgccga taaaatatcc tggagggcca
     4921 accagctgcg ggacaataaa atcgcccagc gtgagtgaga aggtaaagat agagccagcg
     4981 gcaatacccg ggattgccag cggcagcacc acatagcgaa aggtttgtcg tggacgtgcg
     5041 ccgagatcag ctgacgcctg caacaatgac ggcggcaaac gctcaagcgc cgcctgcacc
     5101 gggaggatca tgaacggcaa ccagatatag agaaacacca gaaagcgccc cagcccggaa
     5161 gttgacagcg tattacctcc caccgccggt agcgttagga acgcagtcag cagtggttcc
     5221 agccccagat gttgtaaaaa ccactgcgcc acgccatctt ttgccagcaa taacgtccag
     5281 gcataggctt tcacaatgta gctcgcccac atcggcaaca ttaccgcgat ataaaaaaac
     5341 gctttcattt tcccgctggt atagcgcgcc atataccacg ccattggaaa agccagaatg
     5401 gcgctggcga tagtgactgc cacagccata atcagcgtgc ggagaatgat gtcgtaattc
     5461 gccggattaa acagcgcacg gatattcgcc agcgtcagtt ccggcgttac cgacatggtg
     5521 aaatcgtcaa aggtataaaa tccctgccat aacagtgtca gcagcgagcc gaaatagaca
     5581 atgccaaacc acattagcgg gccaagcagc agtaaaaaca gccccagccc cggattaccc
     5641 cagaagaaac cggacacctt acccagacct ggacgtgaag gtgattgcaa tacgttcatc
     5701 gccattcacc tctcctcaac cagcggtacc atcacatcac gcgaccagga aaccatcacc
     5761 tgttgtccgg gcgtgagcgt ggcgggcagt tcttcgcctg tcatattggc ctgactcacc
     5821 agcagttttt caccgccgtt cagtctcagt tcaaaacggg tcgccgcgcc ctgatactgc
     5881 accgcctgga tcgtgccatt agcctgcact tcgccgggga tgttgaagcg gatatgttcc
     5941 ggtcgcaggg cgaagcttcc cgtcatgcca caaagtttgt ctgccatcag tgcatcaaaa
     6001 acattcgatg tcccgacgaa cccggcaaca aacggcgtgc gcgggcgcat atagagatca
     6061 cgaggggaat cgacctgctc aatgcgtcca ttattgaaaa ccgccacacg atcggacatc
     6121 gataacgctt cgccctgatc gtgggtaacg aagataaaag tgataccgag agactgttgc
     6181 agttttttca gttccagctg catctgctca cgcaatttga gatccagtgc gccgagcggt
     6241 tcatccaaca gcaatacgcg cggttcattc accaatgctc tggcgatagc aacccgctgg
     6301 cgctgaccac cagaaagctg tgacggctta cgttgctgaa caaaaccaag cgccactttc
     6361 tccagcgctt cttgcgccat agcatatcgc tgttttttat ccacgccttt gaccatcagc
     6421 ccataggcca cgttgtcaag aatcgacata tgcggaaaaa gtgcgtagtc ctggaagacg
     6481 gtattaacat cccgctccca cggtggcaga ttgctggcgg atttaccaaa gatagagata
     6541 gccccgccgg aaagctgttc gaagccagca atcaggcgca ggcaggtggt tttgccggag
     6601 ccggacggcc ccagcataga gaagaactca ccatctttta tcgcaatact gacgccatct
     6661 actgcgcgca cgtcaccgta caaccgtgag acgttgtcaa actccactgc gtacgtcata
     6721 aaacacccca gcgaagttag cgaccgccca taatggcaat gtaatcctgc gtccagcgac
     6781 tgtagggaac aaacttgccc ccttctgcta taggcgtttt ccagaaggcg attttatcga
     6841 aatagttaaa accgttggtt tcacaacctt tttcacctaa taacggactg gctttacacc
     6901 cttgcggcac tactggtaac gagccaaacc aggccgccac atcaccctgc acttttggcg
     6961 ttaatgacca gctcatccat ttgtaggcgc aaaccggatg tttcgcttcg ctatgtagca
     7021 tggtggtgtc agcccaaccg gtaacgccct ccttcgggaa aacggtagcg acaggctggc
     7081 cttcggcttt cagggcgttg gcctgatagg gccatgcact ggaagcaacc acaccctcgt
     7141 ttttgaaatc gctcatttgc acggtagtgt catgccagta gcgatggatc aaactatgtt
     7201 gatcgcgcat cactttcagt accgcctgat actgttcttc ggtgagttga tacggatcgc
     7261 ggatgcccaa ctgcggctga gtggctttaa cgaacaacgc agcgtccgca atgtagatag
     7321 ggccatcata agcctgaacg cggcctttat tgctcttgcc gtccggcaga ttctgctcaa
     7381 caaaaaccac ttgccagcta tccggcggcg tcgggaaggt tttagtgttg tacatcagca
     7441 ggttcggccc ccattggtaa ggtgtgccgt aaactttgcc gccaacgtta aaccagtcgc
     7501 ctttaaccac gcgcggatcg agcgctttcc agttgggaat caaggcggta ttaattggct
     7561 gcacgcgttt acccataatc aaacgcagcg aggcatcgcc ggatgccgta accagatcgt
     7621 aacccccttt ggccatcaga ctgaccattt catcggaagt cgcggcggtt ttaacattca
     7681 ccgcgcagcc tgtctctttt tcgaattgcg ttacccagtc gtattgttta tcagtttgtc
     7741 cgcgttcgat gtatcccggc caggcgataa tatccagtcg cccttccggt ttatctaaat
     7801 tggtaggtgg ttcggcggcg tgagcggtca ttattgtcat ggtgagcgca cacaggctgc
     7861 tgcgggcaaa tgtcttgctc ataaagtctt actcctgtcg taattaattg ttcagccgcc
     7921 gtgccgtaaa aatatctcct tgtttaacca tagacggcgg ctagcaggca caccttcgcc
     7981 cctgagaaaa tttaatcagg ttgtgagcta ccgcgcatta tgccgtatgt aaggttatct
     8041 tctggagtat agacaaggag ttaggaaata tgactgctat tcgaaattca tattacccaa
     8101 cctgagagga atttaattaa acgctattac agccgttctt gaataagttt gcctaattgt
     8161 ttaaccgcct gttcttcacg ttctccccac tgccacgcgg tattaaaacg gaaaaaacgt
     8221 gaccagtttt caccggtaga aaacatttta cccggcgcaa tactgatatg atgcgtcagt
     8281 gctgccaggc ttaattcgcc cgcatctaac ggctcgggga gctccagcca gagaaaataa
     8341 ccgctgtcat tatgatgaat tttcacttcc gcaggcagat aacgcagcag tgcttgccag
     8401 gcacgttgtt tacgttccgc aagctggcga cgcaggcgac gaagatgggc gtcgtatcgg
     8461 cgcgtggaaa ggtaatccac cagcgcaagc tgcatcggtg agctggtgga aagcgtactc
     8521 atcaactgca agcgttgaat tttacgtgca tgttttccgg cggcgaccca accaatacga
     8581 aaaccaggca ccagacattt cgaaaacgaa gagcaatgca aaacaccatc gtggcgatcc
     8641 cacgctttcg caggcagcgg tttttcccgt ccaaaataaa gttcgctgta aacgtcatct
     8701 tcaatcagcg ttacgttgta ctgattgagc aacgctacca gccgcgcttt tttctgcggc
     8761 gttaaggtaa atccgagtgg attctggcta ttagtcatca gccagcacgc tttcaccgga
     8821 tactcctgca acgccagttc cagcgccttg agatcaatcc cttctttaac gtccgtcgcc
     8881 accgataacg ccttcagccg taaccgctcc agcgcctgca acgcaccgta gaaacaagga
     8941 ttctctacta tcacccaatc gcccggttca gtgaccgctt gtaaactgag gtttaatgcc
     9001 tctaacgccc cggcagtaat gacaatttca tcgggtgaaa tggtgatgcc ctgtaaggca
     9061 tagcgacgag ctatagcctg acgcagttca gcgtttcctg gcggtaagtt ttcaatcacg
     9121 ctcatcgccg tggcggtttt gcttacctgt gccagcgagc ggttgagttg ttggaggggg
     9181 aaaagtcgcg ggtcgggaaa ggccgaggca aacggaacta ccgacggatc gcgactggcc
     9241 tgcaacatat caaaaatata ggtgttgata tcgactgctt catctcgagt gactggaatg
     9301 actggcgctt tcggcatttt tattgcctgt gtcgcaacgt aataacccga ctgcggtcgc
     9361 gcgataatat gtccctgact ttcgagcaac tgataggcat ggctgacagt cataaagctc
     9421 atgccagaaa gcgccacctg gtcacgcaac gaaggcaaac gatcgccagg ttgccagata
     9481 cccgacgcaa tctgctcgcg taattgttct gcaagctgct ggtatttttt cattatcgaa
     9541 tcgtaattat gtgcagatga ttcggcagtc tatatcagtt gttaaaaatg acaacttttc
     9601 attaactgtt atcaaaccat caccagtgat aactctttgc caagcgcctt agccgcaagc
     9661 tggacggcgt cgatttttgt agcatggtgc aagttaaata agcgagtaat ctcctgttta
     9721 ggtttgccaa ttcgtctggc taactcttgc tgagtgattt ctgactgtaa aaaagcattt
     9781 aacagcaata ctttagaggc gatacttaaa ggcacttcaa taaagtgatc gttgttagtt
     9841 aatggcgaag gcaacgggat tagctcatta tcttcaaaat aaaaatcaaa tgcggtgagt
     9901 aaggcatctt ttgctgcttc catcgcttca gcaacagttt ctccctgcgt caacgcttca
     9961 gggatatcta caaaagaaac cacataaccg ccttccggcg caggagtaag agtgacggga
    10021 taacgcataa ttttgactgt ctctcgcatg tcattaacct tcttatggcg attaatttaa
    10081 accgagttgc ttcaggattg ctttacgcaa tggttctttt atctcatcac tggggtgacg
    10141 cggcatgaca ctgcgtcgcc cttgaaacct gagtttcaaa tggttgctgc cattcgccac
    10201 atcgacgccc tgagattcga gccaacgtct gaactcgctt tgtttcacat cgcctccctg
    10261 ccgctgagta tatcaacaaa gtaaacatta gtgtttacct ccgtgtgtaa tttgctcgaa
    10321 aatagcacta aatcgccgca acggcagaat gcaatgcaaa atcaggaagg aagattccag
    10381 aatttctttg cgctaatttg caaaactgaa taataacctc aagaaacgat cggcatcata
    10441 gcgacaaaag gtaagaaaaa ggtggcagga tgtttacgaa ggcgttatcg gttgtcttat
    10501 taacgtgtgc actgttctca ggacaactca tggcagggca caaaggacat gaatttatgt
    10561 gggtaaagaa tgtggatcat cagctgcgtc atgaagcgga cagcgatgaa ttgcgtgctg
    10621 tggcggaaga gtcggcggaa ggtttgcgcg agcattttta ctggcaaaaa tcgcgcaaac
    10681 cagaagcggg acatcgttga gtcatttgtt gccggatgcg gcatgacgcc ttacccggca
    10741 ggcaatatca tgcagatttg atgagttgtg agtaaccaga aaactggcgt tttacccttt
    10801 tcgcttcggt aaagttttca gcagctcatc cggacttacg gatgccacta cgcttcccgg
    10861 taacggcatt ttcagtatgt gatttttgat tttgccaatg acgtgcatct cgcatgggcg
    10921 gcaatcaaac tttagcgtta atacttcatc gccgttaacc agttgcattg gcgtcgcctt
    10981 ccagcttttg atattgcctt tcgcctgttt cgggcacaga ttgaaggcaa agcgcagaca
    11041 atgcttagtg atcatcaccg ggacttcgcc cttctcttca tgcgcttcat acgccgcatc
    11101 aatcagttgc acaccatagc gatgataaaa ttcacgcgct ttctggttgt aaacgttcgc
    11161 gaggaaactc aaatgcgttt gcggataaac cggcgcagga tcagcaaccg gtttacggct
    11221 gccgcgctgg taactggcaa gacgcgcagc atctagcatg tcggcggctt cacggcggaa
    11281 ctggtttaac agactgtttg gtacaaacag cgcacccggc aaattaattt gcacatcgcg
    11341 ggcgtaatag atggtttgcc ccagttttgc cagaccatcc tttagattgt tcattgcttt
    11401 ttcggcgtta ttggcttcgt cgaactgccc gtccagcgta tgcgtgatgc tgacaccctc
    11461 ttcactggtg agggtcagaa tcagttgttc ctgccagccg cccagttcaa tgtctaccgc
    11521 cacccgacgt tcgctggagg tttttgtcag tgcctgctgc cagttatgat caagattacg
    11581 gtttagtggg tgatgcgggc gaattttgtg caactctgct ggcatttcat tgggccagac
    11641 gcggtactga ttttctccgg ttttctcgac cgtattggca cgaaagccga cgacttcacg
    11701 tttaatcatc acgttcaggc catcgccatt tgccagtggc tcggtaacgg caacatcgag
    11761 atgatctttc gccactttca atacttcacc caccggcagg ccgataaatt tcggcgaatc
    11821 gaacgcgcca atatcgcctt tacgggcatt cacaaaataa tctgtgctac cacggtggaa
    11881 agtcttttcc gtcgatggaa caaagaaatg ttcagtacga cctgatgaag cacgcgtcag
    11941 atcgccacgt tcttcaataa tggcatccag catctggcga taatgggcgg tgatattctt
    12001 cacgtagctc atatctttgt aacgcccttc aatcttgaag gagcgtacgc cagcatcaat
    12061 cagcgcgccg aggttggcag tctgatcgtt atctttcatc gacagcagat gtttttcata
    12121 ggaaaccacc cgcccctgat cgtctttcaa tgtgtatggc aaacggcacg cctgcgagca
    12181 atcgccacgg ttggcgctac gccctgtttg cgcatgagaa atgtagcact gacccgaata
    12241 ggccacgcac agtgcgccat gaataaagaa ttcaatggtc gcgtccgtgg cctggtgaat
    12301 cgcgcggatc tgctcaagat tcagctctcg cgccagcaca atctgcgtga agccaacatc
    12361 agagaggaac ttcgcttttt ctactgtacg aatgtcgcac tgcgtactgg cgtgcagttc
    12421 aatcggcgga atatcaagtt ccagaatccc catatcctga acaatcagcg cgtcgacgcc
    12481 ggtctggtag aggtcggtaa tcagccgttg cgcaggttcc agctcatcat catgcaaaat
    12541 ggtgttaagc gtgacgaaaa tttttgcacc ataacgatgg gcaaatggca ccagttcggc
    12601 aatatctttc aggctattac tggcattatg acgggcacca aaaccagggc cgccgatata
    12661 aacagcatcg gcaccgtgca aaatagcttc gcgggcaatg gcggcatcgc gtgccgggct
    12721 taacagttca agtcgatgag aagatacggt cacattctta tccagtagct gaaaaatggc
    12781 ggctatttta gccacaagcg cgacgttgcg aaatagtttt cccgcttaac tgcgtgggta
    12841 atggatgagg gaatgaaaat gtacgatttg ctccccacca ttacgataga catgcgtaac
    12901 gtctgcagca aatcgagcgc cttctccaca attaaggctc tgccattcac catcaacgca
    12961 cagatcaagt tgcccatcaa tgacgacaac atgttcgatt actcccttct catggggcgt
    13021 cgattcactt attgcaccag gtgccatctg aatcgagaag tgttcaaagc agagttgtgg
    13081 atcataagga aacagcgaag tgatcaccat cacctgctgt tgcggatcat aaactgaagg
    13141 cgtagcggac tgcggcggag aaataaatgt cgaaaatggc acattcaacc cggtggcaat
    13201 tttccataat gtcgcgaccg tcgggctgga ctcattacgc tcaatctgcc ctaacatcgc
    13261 tttagaaacg cccgtcgctt ctgccagtcg cgaaagactc cagccgcgct gctgacgtag
    13321 ttgtttaagt gtggtggata aaaagcgagt gagattttcc atgcgactcc tccggcaaaa
    13381 cggaagttta tcacttgtgc gttataacgg acaaatgcta cggtacctgt atgctataac
    13441 gcacgaggtg accatgcgtc tgttttctat tcctccaccc acgctactgg cggggtttct
    13501 ggcggtatta attggctacg ccagttcagc ggcaataatc tggcaagcag cgattgtcgc
    13561 cggagccacc actgcacaaa tctctggctg gatgacggcg ctggggctgg caatgggcgt
    13621 cagtacgctg gctctgacat tatggtatcg cgtacctgtt ctcaccgcat ggtcaacgcc
    13681 tggcgcggct ttgttggtca ccggattaca gggactaaca cttaacgaag ccatcggcgt
    13741 ttttattgtc accaacgcgt taatagtcct ctgcggcata acgggactct ttgctcgtct
    13801 gatgcgcatt attccgcact cgcttgcggc ggcaatgctt gccgggattt tattacgctt
    13861 tggtttacag gcgtttgcca gtctggatga tcaatttacg ttgtgtggca gtatgttgct
    13921 ggtatggctg gcaaccaagg ccgttgcacc gcgctatgcg gtaattgccg cgatgattat
    13981 tggggtcgtg atcgttatcg cgcaaggtga cgttgtcaca actgatgttg tctttaaacc
    14041 cgttctcccc acttatatta gccctgattt ttcgtttgct cacagcctga gcgttgcact
    14101 cccccttttt ctggtgacga tggcatcgca aaacgcaccg ggtatcgcag caatgaaagc
    14161 agctggatat tcggctcctg tttcgccatt aattgtgttt actggattgc tggcactgtt
    14221 tttttcccct ttcggcgttt attccgtcgg tattgcggca atcaccgcgg ctatttgcca
    14281 aagcccggaa gcgcatccgg ataaagatca acgttggctg gccgctgccg ttgccggcat
    14341 tttttatttg atcgcaggtc tgtttggtag tgccattacc gggatgatgg ctgccctgcc
    14401 cgtaagttgg atccagatgc tggcaggtct tgcgctgttg agtaccattg gcggcagttt
    14461 gtatcaggcg ctgcataatg agcgtgagcg cgacgcggcg gtggtggcat ttctggtaac
    14521 ggcaagtgga ttgacgctgg tcgggattgg ttctgcgttc tggggattaa ttgccggagg
    14581 cgtttgttac gtggtgttga atttaatcgc taacagaaac cgataataaa aagcgccatc
    14641 taacgatggc gcaactggta ttactttttg ttaacgtcaa acatggtggc atcggtcgcc
    14701 atgtcatcaa taacttgttt aatattttcg aaagccattg gcgtactttc gttattcagg
    14761 tctttacctt cgccctgacg aacgactttg ataaccggtt tattagtcgc tgcatcaatc
    14821 agctcacctt caaaatacag gcgagtatcc atggtacggt ggcccgtcgc catttgcgtc
    14881 cccgcaacca ctaatgcaac aggaaccact tcatagaatt gcagcccttc tttgctggta
    14941 tctacaccgg taatggcccc acggaaaatc agactacgcg gcccagcggt ggtaaccagt
    15001 ggtttacgct gcgcaatcgc ttctttcatt tcggtgttgg tataactcaa aattttatcc
    15061 agaactttct gccctacctg ggtagatggt ttcggtaccg gataataagt gactgggttc
    15121 cagacgatgc tgtcatattt gctttgatca aaactcgggt ctacccaacg taaaacaggt
    15181 ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat tgtttaaaaa accagaatat
    15241 ttatctggct gggtgatttt agaggcacaa ccagacagag ccaacaagcc gcataaagct
    15301 gcaactttcg caattgatgt ggtacgcatg aata
//
LOCUS       DAZGAP010000131        15319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGAP010000131 DAZGAP010000000
VERSION     DAZGAP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.132"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            375..820
                     /locus_tag="ACHLAM_003578"
                     /pseudo
     CDS             375..820
                     /locus_tag="ACHLAM_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            864..1132
                     /locus_tag="ACHLAM_003579"
                     /pseudo
     CDS             864..1132
                     /locus_tag="ACHLAM_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1337..2455
                     /gene="ybdK"
                     /locus_tag="ACHLAM_003580"
     CDS             1337..2455
                     /gene="ybdK"
                     /locus_tag="ACHLAM_003580"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO7855048.1"
                     /translation="MPIPDFQVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            2521..2769
                     /gene="ybdJ"
                     /locus_tag="ACHLAM_003581"
     CDS             2521..2769
                     /gene="ybdJ"
                     /locus_tag="ACHLAM_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO7855049.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2834..3202
                     /gene="ybdF"
                     /locus_tag="ACHLAM_003582"
     CDS             2834..3202
                     /gene="ybdF"
                     /locus_tag="ACHLAM_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7855050.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            3296..3949
                     /gene="nfsB"
                     /locus_tag="ACHLAM_003583"
     CDS             3296..3949
                     /gene="nfsB"
                     /locus_tag="ACHLAM_003583"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO7855051.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQVKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLEEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            4057..5304
                     /gene="ybdG"
                     /locus_tag="ACHLAM_003584"
     CDS             4057..5304
                     /gene="ybdG"
                     /locus_tag="ACHLAM_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO7855052.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5385..6761)
                     /gene="pheP"
                     /locus_tag="ACHLAM_003585"
     CDS             complement(5385..6761)
                     /gene="pheP"
                     /locus_tag="ACHLAM_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO7855053.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6863..10006)
                     /gene="cusA"
                     /locus_tag="ACHLAM_003586"
     CDS             complement(6863..10006)
                     /gene="cusA"
                     /locus_tag="ACHLAM_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO7855054.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGQTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(10018..11241)
                     /gene="cusB"
                     /locus_tag="ACHLAM_003587"
     CDS             complement(10018..11241)
                     /gene="cusB"
                     /locus_tag="ACHLAM_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO7855055.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADESLKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(11257..11589)
                     /gene="cusF"
                     /locus_tag="ACHLAM_003588"
     CDS             complement(11257..11589)
                     /gene="cusF"
                     /locus_tag="ACHLAM_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO7855056.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKIIIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11613..12995)
                     /gene="cusC"
                     /locus_tag="ACHLAM_003589"
     CDS             complement(11613..12995)
                     /gene="cusC"
                     /locus_tag="ACHLAM_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO7855057.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            13152..13835
                     /gene="cusR"
                     /locus_tag="ACHLAM_003590"
     CDS             13152..13835
                     /gene="cusR"
                     /locus_tag="ACHLAM_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO7855058.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13825..15273
                     /gene="cusS"
                     /locus_tag="ACHLAM_003591"
     CDS             13825..15273
                     /gene="cusS"
                     /locus_tag="ACHLAM_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO7855059.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQAQRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFMIKLPEKG"
BASE COUNT         3729 a         4097 c         3745 g         3748 t
ORIGIN      
        1 tttattttat tacataatag aaaaaatcta ttatgtgcaa ttatttaata gaataataac
       61 acgtgcaacg aattttaagt gtttcttgag tgacataaaa taagtgtggt gtccatatcc
      121 tcctatcgtg aatcaatata aatgatttac acctaacact ataaaatgtt cagaaagcca
      181 tcgcaataat aaaaaataag acattacgat aatattataa agcattgctt tgttggggga
      241 tgacgtatgt agcaatgaaa gataacaaat gccggtaatg gtacggagaa atcctcagta
      301 ataggcgggt aaaaaagacg ggcatagagt tttgtggtcg actggttgtg ctaacaattc
      361 aggagatcac ctctatgccc gttcgctcat catgccaaaa gtttttccgt gatgatttga
      421 ctccatttca tcagtaccgc cagaatgcca tccttgacgc cactgctgcg cttagtcggg
      481 acgcatccct catccttacc agcattggac ggtatatgcc aggcaatgct caggttaaaa
      541 caagattaag cccatcgatc gcctgccagg caatgacgct ttacaccgcg atatcccggc
      601 tatttttcat aatattaccc ggtttcagcc ccgagacctt acgttgtgcg tcattgccgt
      661 tgactggagt ggttatcctt ctcaggcatt ccatgttctg catactcccc aagtttgcga
      721 cgggcgttct atattacttc tggatcaggt tgtatcgtca ttctggcaac aaattgctca
      781 gttctataag aattttcttg atacgcttgc aatcactatt gctccgaata aaccggataa
      841 aaattacctt taaacacagc accatgaccg ccacaagggc atatttcgtc agcatgttta
      901 tattgcctcc atgcaaagaa gggactacca tccaaattgt ttgggtttgg agtggcagcc
      961 ccaggattga tagaaatatc tcctggggct tcatctttcc agacctcaga gtaaacctga
     1021 aaccggaaag cttcaggcac ccgccgttat cttacccttc cttagcaaaa tggctgttta
     1081 tttatacagt aagtcctgtt tcggacttct caatcctcat ttgatcaact gaagctgaac
     1141 ccgttttatt ctgcatgcct caatcgtcaa aattcttcgc caccagtccg cactgccata
     1201 acttgtcata ttgcaaaatg tagccaattt gcttcagtca ccatcatcaa aaaaagaaac
     1261 attccagaac ttccgcatct cgcccagccg ctccgacttc tatactgaat agaaaacgcc
     1321 aacataagag aaacctatgc caatacccga ttttcaagtc tctgaacctt ttaccctcgg
     1381 tattgaactg gaaatgcagg tggttaatcc gccgggctat gacttaagcc aggactcttc
     1441 aatgctgatt gacgcggtta aaaataagat cacggccgga gaggtaaagc acgatatcac
     1501 cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat atcaaccagg ctgccgggca
     1561 attttcagcg atgcagaaag tcgtattgca ggcagccgca gaccatcatc tggaaatttg
     1621 cggcggtggc acgcacccgt ttcagaaatg gcagcgtcag gaggtatgcg ataacgaacg
     1681 ctatcaacga acgctggaaa actttggcta tctcatccag caggcgaccg tttttggtca
     1741 gcatgtccat gttggctgcg ccagtggcga tgatgccatt tatttgctgc acggcctgtc
     1801 acggtttgtg ccgcacttta tcgccctttc cgccgcgtcg ccatatatgc aaggaacgga
     1861 tacgcgtttt gcctcctcac gaccgaatat tttttccgcc tttcccgata atggcccgat
     1921 gccgtgggtc agtaactggc aacaatttga agccctgttt cgctgcttga gttacaccac
     1981 gatgatcgac agcattaaag atctgcactg ggatattcgc cccagtcctc attttggcac
     2041 ggtggaggtt cgggtaatgg ataccccgtt aacccttagc catgcggtaa atatggcggg
     2101 attaattcag gctactgccc actggttact gacagaacgc ccgttcaaac atcaggagaa
     2161 agattacctg ctgtataaat tcaaccgttt ccaggcctgt cgctatgggc tggaaggcgt
     2221 cattaccgat ccgcatactg gcgatcgtcg gtcactaatg gaagacacct tgcgtttact
     2281 ggaaaaaatc gccccttctg cacataaaat tggcgcatcg agcgcaatag aagcactgca
     2341 ccgccaggtc gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg
     2401 cggctcgctg attgggctgg tgaaaaagca ttgtgagatc tgggctggtg actaagccgg
     2461 gaattgcctt acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt
     2521 atgaaacacc ctttagaaac cttgaccacc gcagcaggca ttttgctgat ggctttcctt
     2581 tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc
     2641 atgttccatc tgatggatct tagccagctt tacactttat tgttttgtct gtggttttta
     2701 gtgctgggcg ctattgagta ttttgttctg cgctttatct ggcgacgctg gttctcgctg
     2761 gcggattaaa cgtcgattat cgccgtactt cgcggcatac tttgcttatt ctccttcgcc
     2821 taaaggaacg tttatggata agcaatcact gcacgaaacg gcgaaacgcc tggcccttga
     2881 gttacccttt gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg
     2941 cggcaagatt tttatgctgt cgtcggagct gcgcggcgtc ccctttatca atctgaagtc
     3001 cgatccacaa aaatccctgt taaatcagca aatatacccg agcatcaagc cagggtatca
     3061 catgaataaa aagcactgga tttcagtgta tcccggcgag gaaatctccg aagcgttact
     3121 tcgcgatctg atcaacgatt cgtggaatct ggtggttgat ggtctggcta aacgcgatca
     3181 aaaaagagtg cgtccaggct aaagcggaaa tctatagcgc atttttctcg cttaccattt
     3241 ctcgttgaac cttgtaatct gctggcacgc aaaattactt tcacatggag tctttatgga
     3301 tatcatttct gtcgccttaa agcgtcattc cactaaggca tttgatgcca gcaaaaaact
     3361 aaccccggaa caggccgagc aggtcaaaac tctcctgcaa tacagcccat ccagtaccaa
     3421 ctcccagccg tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa
     3481 atccgctgcc ggtaattacg tgttcaacga gcgtaaaatg cttgatgcct cgcacgtcgt
     3541 ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga
     3601 agatgccgat ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa
     3661 gttcttcgcc gatatgcacc gtaaagatct gcacgatgat gcagagtgga tggcaaaaca
     3721 ggtttatctc aacgtcggta acttcctgct cggcgtggcg gcgctgggtc tggacgcggt
     3781 gcccatcgaa ggttttgacg ccgccatcct cgatgaagaa tttggtctgg aagagaaagg
     3841 ctacaccagt ctggtggttg ttccggtggg tcatcacagc gttgaagatt ttaacgctac
     3901 gctgccgaaa tctcgtctgc cgcaaaacat taccttaacc gaagtgtaat tctcccttgc
     3961 cgggcatctg cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc
     4021 cgtcatcata ggctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt
     4081 gaagatttag cgggtattga gatcgatcac accacctcga tggtgatgat tttcggtatt
     4141 atttttctga ccgccgtcgt ggtgcatatt tttttgcact gggtggtact gcggaccttc
     4201 gaaaaacgcg ccatcgccag ttcacggctt tggttgcaaa tcattaccca gaataaactc
     4261 ttccaccgtt tagcttttac cctgcagggg attatcgtca atattcaggc ggtattctgg
     4321 ctgcaaaaag gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg
     4381 atgtatgcgc tgctttcagt cttctcgttg ctggatgtga ttttgaatct ggcgcagaaa
     4441 ttcccggcgg catctcagtt accgctgaaa gggatatttc aggggattaa actgatcggc
     4501 gcgattctgg tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattctg
     4561 atcagcggtc ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga tccgattctt
     4621 ggtctggtgg caggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg
     4681 gagatgccga aatacggcgc ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa
     4741 gtacgtaact gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc
     4801 tttaaaaact ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt
     4861 attgatgtca ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg
     4921 catttgttaa agccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag
     4981 caaggttcta cggagtcggt attaaacctg cgccgaatga ccaatattgg aacctttcgt
     5041 gcctatctga acgaatatct acgtaaccat ccgcggattc gtaaagatat gaccttaatg
     5101 gtacgccaac tggctcctgg cgataacggt ttaccgctcg agatctatgc gtttaccaac
     5161 accgtggtgt ggctggaata tgaaagcatt caggctgata tattcgacca catatttgcg
     5221 attgtcgaag agtttggtct gcgacttcat cagtcgccaa ctggcaatga tattcgctct
     5281 ctggcgggtg catttaagca gtaattaaaa aaaccgctct catcgaatgg atgagagcgg
     5341 tttcggatgg ttgacatcat tttgtcggat acggcgcgaa tgccttattt ccgacgcagc
     5401 gttttaaacg ctacaaacag gaatacaatc cacactggca gcagaatcgc tgacaagcgc
     5461 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcgat gcaaagatag
     5521 ttgccgaacg gatagagcag cgccttaaac tgtgtttcac gtccctgacg tcgcatcgcc
     5581 gcgcgaaaac gcagatgcgc caggcagatc ataatccagt tcaacagcag cgttgctacc
     5641 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttaat cagcaccacc
     5701 agcgaagtga tggctccgga aagcatcagc gagttaatcg gcacgccgcg acggctgacg
     5761 cgagtcaaaa acttcggtgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     5821 gagtaaaccc cgctgttata caccgacagc gatgccacca gaatgacgaa gttcagcgca
     5881 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     5941 ttcacttcca cccacggata gagtgccagt aaaaccacca gtgaaccgat ataaaacagc
     6001 agaatgcgat acaccacctg attcactgct ttcggaatgc ttttttccgg atcgcgcgct
     6061 tcagcggcag taatcccaat cagctccaga ccgccgaaag agaacataat taccgccagc
     6121 gacaaaatca gcccattcca gccggtggca aaaaaaccac cgtaacgcca gaggttatcg
     6181 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     6241 ccgatgatcg ccagcacttt aatcagcgca aaccagaatt cggtttcgcc atataagcgc
     6301 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     6361 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     6421 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     6481 tatttatagg caaagtgggc aaatgaaccg gataccggct cctcaaccac catttcgcca
     6541 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtagcccag caatacagcc
     6601 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     6661 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     6721 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     6781 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     6841 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     6901 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     6961 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     7021 gcagaccggc gataatcact gccaccgtca tcgctttcgg gcgcacgcgc aggaccgcgc
     7081 cgtgatacaa cgcctcatcc agcttctgct cgctgaatgt ttgcggatta ttcaacgacg
     7141 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcaa
     7201 ccccggcaag ggcaataaaa ccggtgcccg tcgccacgga aagatgaaag cccatccacc
     7261 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     7321 cgcccacccg acggaacgcc agatataaca gcacgaagat gatcatcagc gtcatcggca
     7381 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     7441 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaga tcgtgaacca
     7501 ccgacaccat gtcacgatcg cgggcatcga tataaatcca gctcgtcggg cgcgcattct
     7561 cggttttcag catcgacggt ccggtagaga ctttaacgtc agccacgtct gccagggtga
     7621 tttgctgctt catcggcgtg aggatcggca actggcgcag tgcctgcggg ctatcgcgcc
     7681 agctttgcgg ataacgcaga ttaattggat aacgagcaat cccttccacc gtttcgccaa
     7741 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     7801 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata acgcccacct tccagacgct
     7861 cagcaagcgc agaagccacg cctggcaccg ttcgcgccac ttcttcaatt tgctccgcca
     7921 tcgcgtcgat atccgccagc acggtgccgg aaactttaat accgatggga cttttaatgc
     7981 cagtcgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     8041 gcaaacgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     8101 actgatcctg cggcttaagc tgaatggtcg tttctaccat ctccagcgga gcagaatcgg
     8161 tggcggtttc cgctttcccg gtttggccaa atacccgcgc cacttccggt acgctcataa
     8221 tcagcttgtc ggttttttgc agcatactcg ccgcctccgc tgcggaaatc cccggcagcg
     8281 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     8341 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     8401 gccagtgcag tactttcagc aacagcggat gataaacacg aatcaaaaag cgattgagcg
     8461 ggttactgct ttccggcgga attttgccac ggatccagta gcccatcagg atcgggatca
     8521 ctacgatcgc cagtagcgcc gcacccgcca tcgcataggt tttggtgaac gccagcgggc
     8581 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac agcgtgataa
     8641 tcagcagact gataaacagc gccggcccca cttcaacaga tgcatcggta atcacctgcc
     8701 agcgcgtttt attatccaac gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     8761 tatgcgcatt ctcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     8821 tgccacccaa cgacataata ttggcgttca gtccctgaaa gtgcatgaca ataaaagcaa
     8881 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     8941 acagcgcaca gactaccgcc accacaataa actcttccag caacttgccg ctgaggttgt
     9001 cgatggcgcg atcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     9061 gcagactact tttcagcgtt tccagtttgt ccttcacggc ggcgattact tctcgggcgt
     9121 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgccg ttcagttcgg
     9181 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     9241 gcacgccatt ttcactggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     9301 agccgctggc tcgcaccata tattccgctt ccgccagttc gatcgacgaa ccacccgctt
     9361 cctggtttga agcatccagc gcgcttttta cttcggcgag actgatgcca tattgcgcca
     9421 ggcgctgagg atcgataacc acctgatact ctttcaccac accgcctacc gacgccactt
     9481 ccgcaacgtc aggaatggtt ttcagctcat atttgagaaa ccagtcctgc aatgagcgta
     9541 aatcggcaag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     9601 cacccgtggc atctggcccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     9661 ggttgaggta ctccagcacc cgcgagcgcg cccagtacgg atcggtgcca tcttcgaaaa
     9721 tgacatacac ataagagtcg ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     9781 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     9841 gaccgggata gctggtttta ataatcacct gcacatcaga gagatccggc agtgcatcca
     9901 ctggcgtgtt aatgatggtc caggtgcccc agatactcag aaacaacgcg cccatcagca
     9961 ccagaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
    10021 atgcgcatgg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
    10081 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagcg
    10141 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
    10201 gtcggcatca acggtaatca cccgctgttc actgccggta tcaatcagcg cctgtgacgg
    10261 aatgagcagc atcggttcgc tggcggtatt gagttgcaac caggcgttca tgcccggttt
    10321 tagcgactcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
    10381 cacgccgggt agcagggtcc atttgcgaat ggtgagtgtt ttatcaggtc gcgccggaac
    10441 ggtgagcgta aactgcgagg catctttcac cagccatgcg atggactccg ggatcgcggc
    10501 agtgatccac accgggtcca taccctgaat tttcgcgacc acgttatctt tggcgatatt
    10561 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
    10621 gcgagtctgg attttttgcg tggcaatcag gcggcgaata tccgcctccg gcattcccgc
    10681 cagtcgcagt cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
    10741 cagtaaatac tcactctgcg cttccaccca gtcgggaatg gtcaggtcga gaagcggtgt
    10801 gcccttttgt actttatcgc ccacggtaag cggatacacc ttatcgataa acccggcagc
    10861 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
    10921 ggcaaaagtc agcggcccgc gcgttacggt cgccgttttc acccccagat tctgcgtctg
    10981 ggtcgggtca atgcgtacac cagacgcaga actctcttca tcggcatatt tcggcaccag
    11041 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg gatacatcgg
    11101 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gcggttccgc
    11161 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    11221 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    11281 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    11341 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcatg gtcatctccg gccagttcac
    11401 cgcggtaatt ggatcgtggt ggatgatgat ttttttgctt tccagatcaa tggtttttac
    11461 cacaccggtg gcgctaatga cctgtggttg tgcctcgctc atggtttcat gatgatgttc
    11521 gttagcctgg gcattaaagc caataacggt aaacagacta aacattgcga cttgcagtgc
    11581 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc gcctaaggcg
    11641 gtatataaag aaatttcatt aacctgacgg gcataattca ggtcaagtaa agtttgtcgg
    11701 gttgcaaata aagaacgctc agcatccagc acttccagat aacttaccgc gccgtgctga
    11761 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    11821 ctaatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    11881 ttctggattt tctgttcata attcaccacc gactgctgct ggcgaatttc ggcgatatcc
    11941 agattggcct ggttacgtcc ggcattaaaa atggggatct caattttggg tataaaattc
    12001 cacatcccgc tgctggcgtt aaataatgac gacagatcgc tgctggcggt tgatattccg
    12061 ctggtcaggc tgatagacgg gaaaaatgcc gcgcgtgcag caccaatatt ggcattagcc
    12121 gccattaacg catgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    12181 aagcccgccg gtaatttaac gctttgcagg ctgtcgctgt ttactgtctg cgcttgcggc
    12241 agcttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    12301 tgacgtttag cgatgtcgct gcgggtactt tcaatcaccc cgcgagcctg ttccagcgcc
    12361 agaacattac tgctacctgt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    12421 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagctgctg attgaaatag
    12481 ctttgcgcga cattagaaac cagcaaaata tgcaccgcgc gctgagcttc ctcagtggct
    12541 aaataatttt ggcgctcggc ttcgctcatg ttctttaagc gcccgaaaaa atcgagatca
    12601 aagctggcgt taaggccagt cgagaactcc cgcgtcgtgg ctgaatcgcc tttaagattg
    12661 ttgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    12721 cgatattgcg cccgcgcttc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    12781 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    12841 gcgttctggt agttatctgc tgcgttaacc aggccgttct ggttgagtga gaactgctgc
    12901 ggcacgggca ttgccgggcg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg
    12961 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    13021 tgccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    13081 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    13141 gcggaggaaa tatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    13201 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    13261 accatctggc gatgaccggt gattatgatc tgataatcct cgatattatg ctgccggacg
    13321 tgaacggctg ggatatcgtg cgcatgttgc gttccgccaa taaggggatg ccgattctgt
    13381 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggcggatg
    13441 actatctggt gaagccgttc gcttttgctg aactgctggc gcgagtgcgc actctactgc
    13501 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    13561 tcgtcagccg caaagtcacc cgcagtggca cgcgcatcac tctgaccagt aaagaattta
    13621 ctctgctgga gtttttcctt cgccatcagg gcgaagtact gccccgctca cttattgcct
    13681 cacaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    13741 ggctgcgtgg caaaatcgac aacgactttg agccgaagct gattcaaacc gtgcgcggcg
    13801 tgggttacat gcttgaggtg ccggatggtc agtaagccgt ttcagcgccc gttttcgctg
    13861 gcaacccgcc tgaccttttt tattagcctg gccaccatcg cggcgttttt cgcctttgca
    13921 tggatcatga tccactcagt aaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    13981 gagattagcg ccactcttga acgggtactt aatcaccctg acgaaacgca agcccaacgc
    14041 ttaatgacgc tggaagatat cgtcagtggt tattccaatg tgttgatttc cctggcagat
    14101 agccacggta aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    14161 gacgccatac ccgataaaga cacgcggggc ggtgaggtgt atctcctttc cggcccgacg
    14221 atgatgatgc cgggccacgg tcacgggcat atggaacaca gcaactggcg gatgattagc
    14281 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat tgcgctttcg
    14341 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatcg
    14401 atcatcagca tcctgatcgt ctttatcgtg ctgttggcgg tacataaagg tcacgcgccg
    14461 atccgcagcg tcagccgtca aatccagaac attacctcga aagatctcga cgttcgcctt
    14521 gacccgcaga ccgtgcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
    14581 gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggacat cgcccacgaa
    14641 attcgtacac caattacgaa tctcataacg caaacggaaa tcgccctcag ccagtcacgc
    14701 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
    14761 aaaatggtca gcgatatgct gtttctcgcc caggccgata acaaccagtt aatccccgaa
    14821 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcatta
    14881 gcggaagatc gcggcgtgga gttgcggttt gttggtgacg agtgtcaggt tgcgggcgat
    14941 ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcga acgccctgcg ttatacgcca
    15001 cccggagagg caattgttgt gcgctgccaa acggtcgatc accaggtgca agttaccgtc
    15061 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctat
    15121 cgcgttgacc cctcccgtca gcgaaaaggc gaaggtagcg gtattggcct ggcgatagtg
    15181 aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc tcgggggaca
    15241 aggtttatga tcaaactacc ggagaaaggt taattagtta gttcaaactc aaagagtgat
    15301 gtatcgcctt ctgttatta
//
LOCUS       DAZGAP010000132        15155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGAP010000132 DAZGAP010000000
VERSION     DAZGAP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.133"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(334..409)
                     /locus_tag="ACHLAM_003592"
     tRNA            complement(334..409)
                     /locus_tag="ACHLAM_003592"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(374..376),aa:Phe,seq:gaa)
     gene            complement(515..1222)
                     /gene="yqgA"
                     /locus_tag="ACHLAM_003593"
     CDS             complement(515..1222)
                     /gene="yqgA"
                     /locus_tag="ACHLAM_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO7855060.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1375..1452
                     /locus_tag="ACHLAM_003594"
     CDS             1375..1452
                     /locus_tag="ACHLAM_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855061.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            1615..3750
                     /gene="speC"
                     /locus_tag="ACHLAM_003595"
     CDS             1615..3750
                     /gene="speC"
                     /locus_tag="ACHLAM_003595"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO7855062.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAAEQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLFGYVLK"
     gene            complement(3800..5056)
                     /gene="nupG"
                     /locus_tag="ACHLAM_003596"
     CDS             complement(3800..5056)
                     /gene="nupG"
                     /locus_tag="ACHLAM_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO7855063.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5258..6337)
                     /gene="mltC"
                     /locus_tag="ACHLAM_003597"
     CDS             complement(5258..6337)
                     /gene="mltC"
                     /locus_tag="ACHLAM_003597"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO7855064.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6402..6677)
                     /gene="yggX"
                     /locus_tag="ACHLAM_003598"
     CDS             complement(6402..6677)
                     /gene="yggX"
                     /locus_tag="ACHLAM_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO7855065.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6705..7757)
                     /gene="mutY"
                     /locus_tag="ACHLAM_003599"
     CDS             complement(6705..7757)
                     /gene="mutY"
                     /locus_tag="ACHLAM_003599"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO7855066.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7918..8637
                     /gene="trmB"
                     /locus_tag="ACHLAM_003600"
     CDS             7918..8637
                     /gene="trmB"
                     /locus_tag="ACHLAM_003600"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO7855067.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8637..8963
                     /gene="yggL"
                     /locus_tag="ACHLAM_003601"
     CDS             8637..8963
                     /gene="yggL"
                     /locus_tag="ACHLAM_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO7855068.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            9147..9866
                     /gene="yggN"
                     /locus_tag="ACHLAM_003602"
     CDS             9147..9866
                     /gene="yggN"
                     /locus_tag="ACHLAM_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO7855069.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10042..11088
                     /gene="ansB"
                     /locus_tag="ACHLAM_003603"
     CDS             10042..11088
                     /gene="ansB"
                     /locus_tag="ACHLAM_003603"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO7855070.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11205..12212
                     /gene="yggM"
                     /locus_tag="ACHLAM_003604"
     CDS             11205..12212
                     /gene="yggM"
                     /locus_tag="ACHLAM_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO7855071.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSADCYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQTNERWKKDTEQLRRNGVI
                     K"
     gene            complement(12268..13569)
                     /locus_tag="ACHLAM_003605"
     CDS             complement(12268..13569)
                     /locus_tag="ACHLAM_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO7855072.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(13569..14072)
                     /locus_tag="ACHLAM_003606"
     CDS             complement(13569..14072)
                     /locus_tag="ACHLAM_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO7855073.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEVS"
     gene            complement(14117..15103)
                     /locus_tag="ACHLAM_003607"
                     /pseudo
     CDS             complement(14117..15103)
                     /locus_tag="ACHLAM_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         4009 a         3638 c         3854 g         3654 t
ORIGIN      
        1 cacaggagtc ttacaacgac catggggtat gttgatgtct gtatggagtc tgcagcgcag
       61 acactggaag atgccctgag tttgtataca gatgagcgca cagtgagcaa gatttgcaaa
      121 ggggggaata aatcttgaca ggctgaatca gcaatgtaag catacagtgt taacgagaac
      181 agggcgtctc tgcaaaagac accctgtttg aggatacgat accgaactca atcagtgtaa
      241 ccacaactta cacaaacgga gaccctttga tgccgcatcc tgtattaacc gcccgtcaag
      301 gatctcatcc cttaaccagc gtgttctggt atatggtgcc cggaggcgga atcgaaccac
      361 cgacacgggg attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca
      421 ttaaacctga ttcgcctcgt ctcgtcaatc aaatttcttc aatttactgc agactgcaca
      481 atctatcatc actttgccgc tattgcacgc attgtcaggc aaaccaggcg gtccaggcgg
      541 cggaaagcgg cattgccaga aggagtgctg gaagcatgtt gaccaccggg aacattttaa
      601 tgccacagat gcgtaatccg gttgccagca gcaataaacc gcctacggcg ctgaagtctg
      661 ccatcatcga cggtgtggtc agcggtaata tcagcgcggc agcccacgcc agcgttaact
      721 ggatgatcag taatgggata ctaattaccg acaccgcaat acccaacgag caggcgaaga
      781 tcatcgccgt aaagaaatcg agaaatgact tggcgattaa aatactcgga tcgccggtca
      841 tcccttcgtt catcgccccg aagatcccgg tgccgctggc gcaaaacagg acaataatcg
      901 cgacataatt ctgaataaaa gattcatgcg ctggcttctt acgtgagtgg cgaaacagat
      961 tttgtgcttt ggtgaccgcg gtattgacgc ctttttccag cagacaaatt tcgccgatta
     1021 atgcaccgag taaggttgct aaaaccatcg ccggaaggtt ggcgcatttc accaccagta
     1081 aaataccaat ccccagcgat gccagaccaa aaatagatgt catggagacg cggatacgtt
     1141 ccggtaaacg ttggctgagc agtgcgccga gaacgccacc cagtaagact gcactagcgt
     1201 tgataaaagg gccgatgacc acaagagttc ctgttaactg tattgtctga ttttcttatt
     1261 atgaactttt tgccttgttg atggcttgtc tggtcgcaaa gtcctggctt gcacgcttta
     1321 gcgaaaggtg ccatgattgc gcgaattttc tcctctctgt acggagtttg cccgatgcac
     1381 gccacctcct tacattctct cgcttatcgc cgtttcgcgc gaaactcctc cctttttctg
     1441 ctcatgcggt gaagttaacg cacgctcact gcaggacaac agtaaaatca gagcgtttct
     1501 gcttttactg atgtctggcg gtcggagctg gtgaccagtt tgacccacat ctcatggggc
     1561 aggtttgtcc atcctgtccg gtactcttac ttccccgaaa cgggttttgc gcttatgaaa
     1621 tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca tcgccgcgtg
     1681 gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac cgctgcggat
     1741 agtcgcagtg gcattcttgc gttgcttaag cgcaccggtt ttcatctacc ggtgtttttg
     1801 tattccgaac atgttgttga attacctgcg ggcgttacgg cggtaatcaa cggcaacgag
     1861 cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt gctgccaccg
     1921 ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc ttgccctgga
     1981 catcaacatg gtgcgttttt taaaaagcat cctgccggac gccattttta cgatttcttt
     2041 ggtgagaacg tctttcgcgc cgatatgtgt aacgctgacg taaaattggg cgatctgctt
     2101 attcatgaag gttcggcgaa ggatgcacag aaattcgcag ccaaagtctt tcatgccgat
     2161 aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac gaatgcgctg
     2221 ttaacgcgtg gcgatctggt gctcttcgac cgtaacaacc ataagtcgaa tcatcatggc
     2281 gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgcaa cccgtttggt
     2341 ttcattggtg gtattgatgc gcactgtttt aatgaagagt atctgcgcca gcaaattcgc
     2401 gacgttgcgc cagaaaaagc agacctgccg cgtccgtttc gtctggcgat tattcagctg
     2461 ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt tgggcatctg
     2521 tgtgattaca ttctgtttga ttccgcgtgg gtcggttacg aacagtttat cccgatgatg
     2581 gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat ctttgtgacg
     2641 caatcggtgc ataaacagca ggcgggattc tcacagacgt cgcagatcca taaaaaagat
     2701 aaccatattc gcgggcaggc gcgtttttgc ccgcataagc ggttaaataa tgcctttatg
     2761 ctccatgctt ctaccagccc gttctatccg ctgtttgccg cgctggacgt taacgccaaa
     2821 attcatgaag gggagagtgg gcgtcggctg tgggcggagt gcgttgcgtt ggggattgaa
     2881 gcccggaagg cgattcttgc gcgctgtaag ctgttccgcc cgtttattcc acccgttgtt
     2941 gatggcaaat tgtggcagga ttatccgacg tcagtgttag ccagcgaccg tcgttttttc
     3001 agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga gcagtatttt
     3061 gtcgatccgt gcaagctgtt actgaccaca ccaggtattg atgctgaaac cggtgaatat
     3121 agcgactttg gcgttccggc gacgattctg gcgcattatc tgcgtgagaa cggtattgtg
     3181 ccggagaagt gcgatctcaa ctccattctg ttcttattaa cgccggcgga aagccacgag
     3241 aagctggcac aactggtggc gatgctggcg cagtttgaac agcatattga ggatgactcg
     3301 ccgctggcgg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta tcgcgactac
     3361 actttgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga cgtcaaagac
     3421 ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa cccccaggat
     3481 gcgcatagcg cttatattcg cggtgaagtg gagttggtgc ggattcgtga tgccgaaggg
     3541 cgaattgcgg cagaaggggc gttgccttat ccaccaggcg tgctttgcgt ggtacccgga
     3601 gaagtctggg gtggggcggt tcagcgttat ttccttgcgc tggaagaagg ggtgaattta
     3661 ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc ggatggcatg
     3721 aaacggttgt tcggttatgt gttgaagtaa gaataaaaaa aacgggtcac cttctggcga
     3781 cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt gcctgtcggg acacgaacgt
     3841 gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa ccagcgaaaa
     3901 tcagccatac ggtctgccag tcggtaatgc cgttttgggt gtacatctca acaactttac
     3961 cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg aacatcccct
     4021 gcgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa ccagagatgt
     4081 tgaagaagtc gaatgcgcaa ccgtaaacga tcatcgacag taccagcagt acagtaccga
     4141 acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc acaatactga
     4201 tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc agaatgaaca
     4261 gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcacg atgaagctgc
     4321 tggcaaacat cggatctttg tcgaagctgt gcaggaaggt gttaccgaac atgttggtaa
     4381 tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata cgcttgtttt
     4441 taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc gcttgctgtt
     4501 tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg gaaagtgctg
     4561 cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc acccacattg
     4621 ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta acgatatcca
     4681 tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc gttggcatat
     4741 aagcaaacga gttaatcaat atcacaagga acatcgcttc cggcgtcgtg acctgtgccg
     4801 ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat ttcgcactta
     4861 accatttgtc ggccacaatc cccagcagcg caggcataaa taccgcggcg atacccagtg
     4921 agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac atataggagc
     4981 cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg attttcagct
     5041 gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt ttgaaaaagc
     5101 acatttgacc gtgcgagatg atgggattct atcaacttcc cggactggtt ttttgttacc
     5161 tgtggcaaat aattgcaaac attcccaatt tgcaaaacga aattgtgatg tggataacat
     5221 ttttacccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg taggattttt
     5281 gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg cgggtcgtca
     5341 gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attggccgcc tgaatcttat
     5401 cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg atgacggcat
     5461 aacgccggga tgtagggtta tcaattccgc cgagataaac gttgttcagc atcgccagat
     5521 acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg ctcggcgtgc
     5581 cggatttccc ctgcgaacgg aacacatctt tcccggcagt atgttgtacc acctgcatta
     5641 accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggatgactcg gtctgcataa
     5701 tcgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg accatgccga
     5761 gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg acgctgtaga
     5821 tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa tccgcgaagt
     5881 tgctcgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc tgaccgtaaa
     5941 ggaaaggttc tttcgaaatc gtaatatcat caacgtcgga atagagatcg accgaactcg
     6001 gatcgtcccc catcagcaac gttttgataa ttgcccggcg cagatgcgcg gcaggttctg
     6061 tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg ctgcgggtct
     6121 gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc tctttgaagc
     6181 cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa ccgttggtat
     6241 ctttgaccca ggcttcgtta taggtatcgc cttttttggt cgtcgaacag gagatgagca
     6301 acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg tgttgtgttt
     6361 gtttttatgc aagttgccgg aggcgtgctc cggcactgtt tttatttttt atcttccggc
     6421 gtatagccct caatatgcac ctctttaccc tcgaacagga agttgaccat ctcctgttcc
     6481 agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat cagcatggtt
     6541 tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat gcgttttccc
     6601 agctcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag gaaagtacaa
     6661 aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac cggcgcgcca
     6721 gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga cggcggttgc
     6781 gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga cgacacggga
     6841 agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa cgcggtcagc
     6901 tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa actttcttcg
     6961 tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg acgctgcgcc
     7021 agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc cggcagcgtc
     7081 tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc aatacatccg
     7141 ttttgtagcg gacagagcga gcatttcggc ttcgagcgcg tgcaaatcat cgcacccaaa
     7201 tccatcatcg cctgattaaa ccgctccacg ccgaccgcgg gcgtcacctg ttcgctcaaa
     7261 ctccacagtt tattctcgac ctcttttttc ccaggccagc cgcttacagc atagcagcgc
     7321 gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag agaaagcgag
     7381 agaattgcgc ctgcggtgga acgcccgacg cccggtaacg ccgcgacttc ttcaaaggtt
     7441 tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgccttatg cagattgcgc
     7501 gcgcgggcgt aatagccaag cccggtccac aagtggagaa cttcgtcgag cggcgcattg
     7561 gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg gataacggtc
     7621 gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg cgtcttgtca
     7681 atttgccagg gcagcgtttt ccgcccgtat ttatcgtacc agtccagaac ctgggctgaa
     7741 aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat tgcagcacag
     7801 cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc ttgcatccgg
     7861 caactaactt tggataatgc ccgttttcag aacactttca caagcgacta aatctttatg
     7921 aaaaacgacg tcatctcacc ggaatttgat gaaaacggcc gcccactgcg ccgtatccgt
     7981 agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct ggaaaactac
     8041 tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc gctttttggc
     8101 cgagaagcgc cggtgacgct tgagattggt ttcggcatgg gggcgtcgct ggtggcaatg
     8161 gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc gggcgttggt
     8221 gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat gtgtcacgat
     8281 gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt gcagctcttt
     8341 ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca ggtgccgttt
     8401 gccgaactgg taaaaagcaa actgcaactg gggggcgtat tccatatggc gaccgactgg
     8461 gaaccttatg cggaacatat gcttgaagtg atgtcttcta ttgacggtta taaaaacctg
     8521 tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa atttgaacaa
     8581 cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt gaaataatgg
     8641 caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc caggaattag
     8701 gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt gataaaaccg
     8761 ttgatgactt tattaacgag gttatcgaac cgaacaaact ggcctttgac ggcagcggtt
     8821 atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc gaagaacatc
     8881 aggcgattgt gcgtaagtgg ctggaagagc gcaaactggg cgaggtacgc accagcgaac
     8941 ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca aaactgcctg
     9001 atgtgctacg cttatcaggc ctataaagat gcacgatcga gtaggctgga taaggcgttt
     9061 acgccgcatc cggcatggaa aacgcgcact ttgttatcaa tctggggcca gcaaatgctg
     9121 gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg gcagcacttt
     9181 cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg cgtgacgatg
     9241 tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc
     9301 cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc cagcgcgagc
     9361 aggcgaagga ttatcaggct gaactccgta gcaccctgcc gtggattgat gaaggcgcga
     9421 aaagccgcgt cgaaaaagcc cgtattgcgc tggataaaat tatcgttcag gagatgggcg
     9481 aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa gagcagatga
     9541 accgcattat cgaaacgcgc agcgatggcc tgacgtttca ctataaagcc attgatcagg
     9601 ttcgcgccga aggccagcaa ttagtgaatc aggcaatggg tggaatttta caggacagca
     9661 ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta cagaatgtgc
     9721 tgggaagcct gggcggtctg caatcctcaa tccaaaccga gtggaaaaag caggaaaaag
     9781 atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa gatagccgca
     9841 aagccctggt cgggaattta aaataatcct ctattttaag acggcataat acttttttat
     9901 gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca aaatatattc
     9961 acgttgtaaa ttgtttaacg tcaaatttcc catacaaagc taagggataa tgcgtagcgt
    10021 tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc cgcactggtt
    10081 atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc aactggcggg
    10141 accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg taaagttggc
    10201 gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt taaaggcgag
    10261 caggtagtga atattggctc ccaggacatg aacgatgatg tctggctgac actggcgaaa
    10321 aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttacccacgg taccgacacg
    10381 atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg
    10441 gtcggcgcaa tgcgcccgtc cacgtccatg agcgcagacg gtccattcaa cctgtataac
    10501 gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt ggtaatgaac
    10561 gacaccgtac tggacggtcg cgatgtcacc aaaaccaata ccaccgacgt agcgaccttc
    10621 aagtctgtta actacggtcc tctgggatac attcacaacg gtaagattga ctaccaacgt
    10681 accccggcac gtaagcacac cagcgatacg ccgttcgata tctctaagct gaatgagctg
    10741 ccgaaagtcg gcatcgttta taactacgct aacgcatccg atcttccggc taaagcactg
    10801 gtagatgcgg gctatgatgg cattgttagc gctggtgtgg gtaacggcaa cctgtataaa
    10861 accgtatttg acactctggc gaccgccgcg aaaaacggca ctgcagtcgt gcgttcttcc
    10921 cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa atatggcttc
    10981 gtggcctctg gcacgctgaa cccgcaaaaa gcgcgcgttc tgctgcaact ggctctgacg
    11041 caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc acctcgcccc
    11101 ggtaacgtgc cggggctttt tcacttcaga cttacgtcca ttgccaattt tcattaccct
    11161 gatgataatc actggaataa attattccgc gcgagggttt tcgggtgaaa aagcaatgga
    11221 ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat ggcgaaccgc
    11281 caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt ctcaagcttg
    11341 atgtcatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg tcagcggaag
    11401 gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca gattacgaac
    11461 tgctcgaaca gacctggacg aaagataaac cgataaaatt ctcggcgatg ttaaccagta
    11521 aaggaacgcc cgcgtctggc tggtcggtga acttttactc ttttcaggcg gcagccagcg
    11581 atcgtggacg ggtggtcgac gatatcaaaa cgaataataa atatctgatc gtgaatagcg
    11641 aagatttcaa ttatcgcttt agtcagcttg aatcagcgtt gaataaccag aagaactcga
    11701 ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg gcgcagaaag
    11761 cagcggatgc atattgggga aaagatgcaa acggtaagca aatgacccgc gaagacgcct
    11821 ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc gaggcgttcg
    11881 cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat aaacagagtg
    11941 cagattgtta tgaagtgccg attcagcaga agcgagattt cgatatcaac gaacaacggc
    12001 gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac tggataacgc
    12061 tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt aaaaaagtcg
    12121 cgattttgat gaaaattgat gatatcaacc agactaatga acgctggaaa aaagataccg
    12181 aacaattgcg gcgcaatgga gtgattaagt aactcatgtt aagtaccttt gccggataat
    12241 ataaaaagtt atccggcaaa ttatttttta cattagccct agtagctgtg gaatgaacaa
    12301 ggatatttgc gggatgtaag tgatcatcat caacgcgatt aacaatgcgg cataaaatgg
    12361 cagcaatggt ttgatcaaat gttgaatctt cacacctgaa atagaacaac caacaaatag
    12421 cgcactaccc accggcggtg ttaacagccc aatacaaagg ttagccacca tcataatacc
    12481 gaaatgtacc ggatccattc ccagctcttg tgcaatcggc aagaagattg gtgtgaagat
    12541 tagtacagcc ggtgtcatgt ccataaagat gccgacaatt aataagacga tattaatcag
    12601 cagcaatatg accagtgggt tatcagaaat ccccatcaac gcgtcgctga tcatataagg
    12661 aatatcagca ttggtcattg cccaggacat gcccacggaa aaaccgatca acagtaatac
    12721 aatcgaagtg gtaacaaccg attccagaat cagtttgggt aaatcgcgcc attttacttc
    12781 ccggtaaatc agcaccgaga gaataaatgt gtagacaacg gcaattgctg acgcttcagt
    12841 tgcggtaaaa atgccgccca gaatgccgcc cattacaatg aacaccagaa gcaaacttgg
    12901 taaggcatcc agaaacgctt tacatgcctg agcaaaggtt gctctttctg aaagtgggta
    12961 cccacggcgt ttagcaataa tgccgcagac tatcattacc gcgagaccca ttaagatccc
    13021 tggcaaatat cccgccataa atagcgatgc tacagaaacg ccgccagctg tcagggaaaa
    13081 cacaattaac acgtttgagg gcggtatcag taatccagta atacaagatg agacgtttac
    13141 agcggtagaa aatgccggat cataaccttc tttggtctga atgggattca gtgtgccacc
    13201 aacggcagca gctgccgcaa cggctgaacc agaaatggaa ccaaacatca tattcgccag
    13261 gacgttcaca tgaccaagcg aaccaggaac gcggccaacc attacctggg cgaggttaat
    13321 caaacgtatc gcaataccgc cgctgttcat cagcgttccg gcaaagatga aaaaggggat
    13381 tgccaatagg gcaaagttat ccagtccatt agccagtcgc tgcgaaacag tgattgccgc
    13441 gatatcccag ggaaacatca acagcattga agctacagtg gcaataccga ttgagaacga
    13501 aatgggcaca ccaataaaga ccagcacaaa aaaacagcca aacagtgtaa gcgcgatata
    13561 actatccatt aactgacctc ctgcgcaggt tgtttcagtt gttgaatgct ttgattaata
    13621 aaacacaagg cataaaacat catcaccaat ccacttaatg gcagaatgat atatacgtat
    13681 cccatcggta tttgcatcgc ggcagatacc tgtgatgttg ccagtgtttt atcaattaat
    13741 ttcaggccac cagtcacaat gaccgaaccg gcaaaaccta aaataagaac gttaatgact
    13801 atactaagaa gtaattgctt gcgcttattc agggcgagag caaataaatc aatagagaga
    13861 tgtctctgcg cgccgacggt atatgcagca cctaataatc ctacccagat catcaaaaag
    13921 cgtgccagct catcggttaa tgtacttggc tggttaagta cgtagcggct aaatacttgc
    13981 cagactacgc atacaaccag agcaagcatt acggcgacag aaaacgcggc aatgacgcgg
    14041 tctaccgcaa gtttcaggcg gttaaatatc attgtaagca tcctggaaaa tcagggccag
    14101 agactggccc gaaatattat tgagcagcgt tgtcgaattt ctctaacaaa gcagcttgtt
    14161 ttggatcttt tttgaagtcg tcaaacagag gctgaactgc ggcacggaat ggggctttat
    14221 caaccttaac gatttcacca cccatggctt tcgcctgagc acgtgtgtca gcgtcaattt
    14281 tttcccacaa tttttgttga taagcttcgg atttcttcgc cgctgtttcg aggatttgtt
    14341 gctgttctgg ggttaattta ttccatgttt tggtggagat aaccaaaaag tcaggaattg
    14401 aggcatgttc atcttcagaa aagactttgg caatttcgat atgtcgagtc tgcacccagg
    14461 aaggcacgtt attttctgcg ccatctacta caccttgctg catcgcagta taaacttcgc
    14521 caaatgaaat tggagtgggt gagccaccca tcagttcaat cattttgatg gtggtcgggc
    14581 ttggctgaac acgaattttc agccctttaa gatcttctgg cttagtaatc ggttttttcg
    14641 catagaaact gcgtgtaccc gctacataag ctgataaagc aaaaaagcct ttatcttttg
    14701 ttgaatccat aatttcttta ccaacttcac caaaaaccac tttgttgaag tgcgcctgat
    14761 ctttaaataa gaacggcaaa ttatagatag cataaatatt atcaaaagac tccagatcac
    14821 tggcggaacc tttggtcata tccagtgcac cattttgcaa caactacata gtttctcgcg
    14881 cattacccat ctggctgctg gggtaaatac ggatcaccat attacctttt gacagctgtt
    14941 ttacttcttt cgccagctct tcaaaactct gatgtacaac atgacttctt tccaggttgt
    15001 gtgctaactt taacgtcact ttttctgcgg cgtttacgcc agtgctaacc agagccagca
    15061 aggatgcgca gactaatgag cgggtaaatg acctcatttt cataaaaaaa ctccctgttg
    15121 aagaatggaa attggttgat atctaattgt gctta
//
LOCUS       DAZGAP010000133        15135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGAP010000133 DAZGAP010000000
VERSION     DAZGAP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.134"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..519
                     /locus_tag="ACHLAM_003608"
     CDS             <1..519
                     /locus_tag="ACHLAM_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7855074.1"
                     /translation="IFNVDIRTIDRWQTQGLPQISGGGKGVEVIFDSAKAIEWYAQRE
                     ADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNAREEGLVLETE
                     LFTYIFQRVAQNISGILVRVPQTLQRKYPEISPAHLDAMKTEIAKASDVASEAGENVR
                     RWIDDFRRTEGG"
     gene            491..2419
                     /locus_tag="ACHLAM_003609"
     CDS             491..2419
                     /locus_tag="ACHLAM_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7855075.1"
                     /translation="MISDELRAANSAGAIATGLLALKIPVPLTTVQWADRHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDRIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVSVLLDLAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2403..2609
                     /locus_tag="ACHLAM_003610"
     CDS             2403..2609
                     /locus_tag="ACHLAM_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO7855076.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2606..4198
                     /locus_tag="ACHLAM_003611"
     CDS             2606..4198
                     /locus_tag="ACHLAM_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7855077.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRSRFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETRPRET"
     gene            4188..5693
                     /locus_tag="ACHLAM_003612"
     CDS             4188..5693
                     /locus_tag="ACHLAM_003612"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7855078.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALSAEVCQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            5730..6077
                     /locus_tag="ACHLAM_003613"
     CDS             5730..6077
                     /locus_tag="ACHLAM_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7855079.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            6135..7163
                     /locus_tag="ACHLAM_003614"
     CDS             6135..7163
                     /locus_tag="ACHLAM_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7855080.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7215..7598
                     /gene="gpFI"
                     /locus_tag="ACHLAM_003615"
     CDS             7215..7598
                     /gene="gpFI"
                     /locus_tag="ACHLAM_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7855081.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEPLNNTDAPDDVKAVRVRKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSSLAMAMIQHGTAEYA"
     gene            7591..7944
                     /locus_tag="ACHLAM_003616"
     CDS             7591..7944
                     /locus_tag="ACHLAM_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7855082.1"
                     /translation="MRDVQNAFDAALVGVDSTIVEVMGIRVQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            7960..8535
                     /locus_tag="ACHLAM_003617"
     CDS             7960..8535
                     /locus_tag="ACHLAM_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7855083.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTTINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            8532..8927
                     /locus_tag="ACHLAM_003618"
     CDS             8532..8927
                     /locus_tag="ACHLAM_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7855084.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            8935..9684
                     /locus_tag="ACHLAM_003619"
     CDS             8935..9684
                     /locus_tag="ACHLAM_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7855085.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            9701..10123
                     /locus_tag="ACHLAM_003620"
     CDS             9701..10123
                     /locus_tag="ACHLAM_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7855086.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQGTTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            10150..10563
                     /locus_tag="ACHLAM_003621"
     CDS             10150..10563
                     /locus_tag="ACHLAM_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7855087.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPVCEEEQTEMPDEEDMLMQKAAGVAGGVRFCGVGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            10544..13156
                     /locus_tag="ACHLAM_003622"
     CDS             10544..13156
                     /locus_tag="ACHLAM_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7855088.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDVRIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHKDETLEYKRQLAELGDKV
                     EYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPQA
                     LAQVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRVEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            13153..13482
                     /locus_tag="ACHLAM_003623"
     CDS             13153..13482
                     /locus_tag="ACHLAM_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7855089.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRTAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            13482..14180
                     /locus_tag="ACHLAM_003624"
     CDS             13482..14180
                     /locus_tag="ACHLAM_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7855090.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            14191..14934
                     /locus_tag="ACHLAM_003625"
     CDS             14191..14934
                     /locus_tag="ACHLAM_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7855091.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCMNISAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFGSSVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            14931..>15135
                     /locus_tag="ACHLAM_003626"
     CDS             14931..>15135
                     /locus_tag="ACHLAM_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7855092.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAC
                     LHEQLGEGAVIHIVPRLAGAGGNG"
BASE COUNT         3446 a         3615 c         4865 g         3209 t
ORIGIN      
        1 atttttaatg tcgatatacg cactattgat cgctggcaaa ctcaggggct ccctcagata
       61 tctggtggag gaaaaggtgt tgaggtcatc tttgattctg cgaaggcgat cgaatggtat
      121 gctcagcgcg aagctgatat tgaaaatgaa aaactccgta aagaggtcga ggatttgagg
      181 gctgccagcg aatcagacct tcagcccggc accattgatt acgaacgtta ccgactgacg
      241 aaggcacagg cagatgcaca ggagctgaaa aatgctcgtg aggaaggcct tgtcctcgag
      301 acggagttat ttacctacat ctttcagcga gtggcacaga atatatctgg gatccttgtc
      361 cgtgtccctc agacactgca gcgtaaatac cctgagatat cacccgcaca tcttgatgct
      421 atgaaaactg aaatcgcgaa agcatccgat gtggcttctg aagccggtga gaatgtgcgc
      481 aggtggattg atgatttcag acgaactgag ggcggctaat tctgcaggag cgatagcaac
      541 cggccttctt gcgctaaaaa ttcctgtccc cctgacgaca gttcagtggg cagatcgaca
      601 ctattacctt ccgaaagagt catcttacac cccggggcgg tgggaaacac tgccatttca
      661 ggttgccatc atgaacagca tggggaatga ccggatccgc acggttaatc tgattaaatc
      721 tgcccgtgtt ggttatacaa agatgttgct gggagtggag gcttatttta ttgagcataa
      781 atcacgcaac agccttcttt tccagcctac ggattctgct gctgaagatt ttatgaaatc
      841 tcatgtggaa cccacgatca gggatgtgtc ggttttactc gatcttgcac cgtggtttgg
      901 gcgtaaacat cgtgataata ccctcacgct gaaacgcttt tcatcgggtg tgggtttctg
      961 gtgcctgggt ggtgcggcag caaaaaacta ccgtgaaaaa tccgtggatg tggtctgcta
     1021 tgacgaactt tcctcgttcg agccggatgt tgaaaaagag ggctcgccaa ctctgctggg
     1081 ggataagcgt attgagggct cggtatggcc aaaatccatt cgcggctcga cgcctaaaat
     1141 aaaaggcacc tgccagatcg aaaaagcggc caacgagtcg gcgcatttca tgcgttttta
     1201 tgtgccctgc ccgcactgtg gggaggagca gtatctgaaa tttggcgatg aatccacgcc
     1261 ttttgggctt aaatgggaga aggacagtcc cgaaagtgtt ttctacctct gtgaacatca
     1321 tggctgcgtg atccatcagt ctgagcttga ccagagcaac gggcggtgga tctgtgaaaa
     1381 cacggggatg tggacccgtg acggtctgac gtttttcagc gctgcgggta atgaaattcc
     1441 gccgccgcgc tccatcacat tccatatctg gacggcgtac agtccgttca ccacctgggt
     1501 acagattgtc tatgactggc tggatgcact gaaagatccc aacggcctga aaacttttgt
     1561 gaacaccacg ctgggcgaga cctgggaaga ggccgtgggc gaaaaactcg atcaccaggt
     1621 gctgatggat aaggttgtgc gttacacggc ggcggtacct gcccgggtgg tttatctgac
     1681 ggcgggcatt gactcgcagc gtaaccgttt tgagatgtat gtctggggat gggctccggg
     1741 agaggaagcc tttctggtgg ataaaatcat cattatgggg cgtcctgatg aggaagagac
     1801 tctgttacgt gtggatgcgg cgatcaacaa aaaataccgc catgcagacg gaaccgaaat
     1861 gaccatttcc cgtgtctgct gggacaccgg ggggatcgat ggcgaaatcg tttatcagag
     1921 gtcaaaaaaa cacggtgttt tccgggtgct gccggtaaaa ggcgcatctg tctatggcaa
     1981 gccggtgatc accatgccaa aaacccgcaa tcagcggggc gtgtatctgt gtgaagtggg
     2041 gacggacacc gcaaaagaaa ttctctatgc ccgtatgaaa gccgatccca cgcctgcgga
     2101 tgaagccacg tcgtatgcca tccgttttcc tgatgatccg gagatttttt cgcagacaga
     2161 ggcgcagcaa ctggtcgcgg aagagcttgt ggagaagtgg gaaaaaggaa agatgcgtct
     2221 gctgtgggat aacaaaaagc ggcgtaacga agcgctggac tgcctggtgt atgcctacgc
     2281 ggcattacgt gtgtccgtgc aacgctggca gcttgatctg gctgtactgg caaaatcccg
     2341 ggaagaagag acgacccggc caaccctgaa agaactggca gcgaagctgt ccggaggagt
     2401 gaatggttac agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg
     2461 gtaaacgggt ggtgtctgtc cagaaagatg gtcgcagaat tgaatatacg gcggcttctc
     2521 tggatgagct taaccgtgcg atcaatgatg cggagtcggt actggggaca acccgacgtc
     2581 gccgtcgtcc gctgggagtg aggttatgaa acgaacgcct gtcctgattg atgtgaacgg
     2641 cgttccgctt cgggagagcc tcagctacca cgggggcggt gcaggatttg gtgggcaaat
     2701 ggcagagtgg ttgccaccct cgcagagtgc cgatgcggcc ctgctgcccg cgttgcgtct
     2761 ggggaatgcc cgtgcagatg atctggtacg caataacggg atagcggcta atgcggtggc
     2821 cctgcataag gatcatattg tcgggcatat gtttctgatc agctaccgtc cgaactggcg
     2881 ctggctgggg atgcgggaga ctgcggcaaa aagctttgtc gatgaggtgg aggcggcctg
     2941 gtcggaatac gccgaaggga tgtttggcga gatcgacgtg gaaggaaaac gcacgttcac
     3001 ggaatttatc cgtgaaggcg tgggcgttca tgcgtttaac ggcgaaatct ttgtgcagcc
     3061 ggtctgggat acggaaacca cgcagttatt ccgcacgcgt tttaaagccg tgagtccgaa
     3121 acgggtggac acgccaggac acggtatcgg gaatcgtttt ctgcgggccg gggtggaggt
     3181 cgatcgatat gggcgtgccg ttgcgtacca tatctgtgag gatgattttc ctcgctccgg
     3241 gagtggacga tgggaacgga tcccgcgtga acttcccacc gggcgtccgg ccatgctgca
     3301 tattttcgag ccggtggagg acgggcagac ccgtggggcc aatcagtttt acagcgtcat
     3361 ggaacggctg aagatgctcg attccctgca ggcaacacag cttcagtcag ccattgttaa
     3421 agccatgtat gcagcgacga ttgaaagtga acttgatacc gaaaaggcct ttgaatatat
     3481 cgcgggggca ccgcagggtc agcaggataa tccgctcatt aatattctgg agaagttctc
     3541 cagttggtat gacacgaatc acgtgacgct gggcggtgtc aaaattccgc accttttccc
     3601 cggtgatgat ctgaaactgc agaccgcgca ggattcagac aatggatttt ctgcgcttga
     3661 acaggcgctg ctgcggtata tcgccgccgg tcttggcgtt tcctacgaac agttgtcccg
     3721 tgattactcg aaggtcagtt actcaagtgc ccgcgcctcc gccaatgagt cgtggcgcta
     3781 ttttatgggg cggcgaaaat ttattgcggc ccggctggcc acgcagatgt tttcctgctg
     3841 gctggaagag gcacttcttc gggggattat ccgtccgcca cggtcacgtt ttgattttta
     3901 tcaggcgcga tcagcctggt cacgggcaga gtggattggt gccggaagaa tggccattga
     3961 cgggctcaag gaggtccagg aatcagtgat gcgcattgag gccggactga gcacgtatga
     4021 gaaagagctg gcgctgatgg gtgaggatta tcaggacatt ttccgccagc aggtcaggga
     4081 atccgcagag cgggaaaaag ccggactctc acgtccggtg tggatagcac aggcgtatca
     4141 gcagcagata gcggagagcc gcaggccgga agaggagaca agaccacgtg agacgtaatc
     4201 tttcacacat tattgccgca gcattcaatg aaccgctgct tctggagccc gcctatgcgc
     4261 gggttttctt ttgcgcgctc gggcgcgaga tgggggcagc aagtctttcg gtaccacaac
     4321 agcaggtgca gcttgatgct cccggaatgc tggctgaaac ggacgagtac atggccggag
     4381 gtaaacgacc ggcccgtgtt taccgggtgg tgaacggtat tgctgtactg ccggtgaccg
     4441 gcacgctggt gcaccggctg ggtggcatgc ggccattttc cggaatgaca ggctatgacg
     4501 gcattgttgc ctgtcttcag caggcaatgg cggacacctc tgtccggggc gtactgctgg
     4561 atattgacag tccgggcggg caggccgccg gtgcgtttga ctgcgctgac atgatttacc
     4621 gcctccggca gcagaagccg gtctgggcac tgtgtaatga cacggcctgt tctgcggcca
     4681 tgctgctggc gtcggcctgc tcccgacggc tggttaccca gacatcccgt atcggctcca
     4741 ttggcgtgat gatgggccat gtcagctatg ccggtcatct ggcgcaggcc ggggtggata
     4801 tcacgctgat ttatgccggg gcgcataagg tggatggcaa tcagtttgaa gccttatcgg
     4861 cagaggtgtg tcaggacatg cagcagcgca ttgatgcggc gcaccggatg tttgccgaaa
     4921 aagtggcgat gtatacgggg ctgtctgtgg aagctgtcac ggggacagag gctgccgttt
     4981 ttgaaggtca gtccggtatt gaggccgggc tggcggatga gttaatcaat gcgtcggatg
     5041 ccatcagcgt gatggctgcg gcgctgaaca cacatgatac aggaggcact atgccgcaat
     5101 taactgcaac ggaagccgcc gcgcaggaga accagcgagt gatggggatc ctgacgtgtc
     5161 aggaagcgaa aggacgtgaa cagcttgcca cgatgctggc aggacaacag ggcatgagcg
     5221 ttgaacaggc ccgggcgatt ctggctgcgg cagcaccaca gcagccggtg gcatccgcgc
     5281 agagtgaagc cgatcgcatt atggcgtgtg aagaagctaa aggtcgtgaa caactggcgg
     5341 caacgctggc ggcgatgccg gagatgacgg tggaaaaagc ccgcccgatc ctggcggctt
     5401 caccgcaggc ggatgccgga ccctcactcc gtgatcagat tatggctttg gatgaggcaa
     5461 aaggggctga ggcgcaggct gaaaaactgg cggcgtttcc cggaatgacg gtggaggctg
     5521 cccgcgacat tctgtcctca tcgccggata aagcagaacc ggtttctgca tccacaaccg
     5581 ccatgtttga acgcatcatg gcgaaccatt caccggcagc cgtgcagggt ggcgtgtcac
     5641 agacgtcggc agacggtgat gcggacgtga aaatgctcat ggccatgcca tgaagtcagt
     5701 gctgaccatc aatatgaggt ttttacaata tggtaacgaa aaccatcact gaacaacgtg
     5761 cagaagtacg tatttttgcc ggtaatgatc cggctcatac cgccacaggc agcagcggga
     5821 tttctcaggc aacaccggca ctgacgcccc tgatgctgga tgaagccagc gggaaactgg
     5881 tggtctggga cggacagaaa gccggtagtg cggctggcat actggtactg ccgcttgaag
     5941 gcacagagac ggtgctgacg tattacaagt cgggaacctt tgcgacggag gcaatccgct
     6001 ggcctgaaag tgtggatgaa cacaaaaagg ccaacgcctt tgtcggcaca gccctgagtc
     6061 acgcggcgct gccgtaacac gttatcaggc caccgcgttg gcctgactga tttctgaatg
     6121 aaaggaactg atttatggga ttgtttacga cccgccagtt actcggttat accgaacaaa
     6181 aagtgaaatt tcgtgcgctg tttctggaac tgtttttccg ccgtacggtg aatttccaca
     6241 ccgaagaggt gatgctggac aaaattaccg gaaaaacgcc ggtggcagcc tatgtctccc
     6301 cggttgttga aggaaaagtg ctgcgtcatc gtggtggtga aacccgcgtg ttacgtccgg
     6361 gctacgtcaa gccgaaacac gaatttaatt accagcaggc ggtggagcgc cttcccggtg
     6421 aagatccggc gcagctgaac gacccgacct accgtcgtct gcgtatcatc accgataacc
     6481 tcaaacagga agagcacgcc attgtccagg tggaagaaat gcaggcggtg aatgccgtgc
     6541 tgtatggcaa atacaccatg gaaggggagc agtttgatac tgtcgaggtg gatttcgggc
     6601 gctctgaagg aaataacatt gagcaggctg acggtaaaaa atggtctgag caggaccgtg
     6661 atacgtttga tccgacgcat gatattgacc tctactgcga tcaggccagc ggccttgtga
     6721 atatcgccat tatggacggt acggtctggc gtctgctgaa tggctttaag ctgttccgcg
     6781 aaaaactgga tacccgtcgc ggctcaaatt cacaactcga aacggcagtg aaagatctgg
     6841 gcgcagtggt gtccttcaag gggtattacg gcgatctggc cattgtggtg gcgaaaacgt
     6901 cttatgtggc agaggacggt accgaaaaac gttatctgcc ggagggcacg ctggtcctgg
     6961 gaaatacgtc agcggagggc atccgttgct atggtgccat tcaggatgcg caggcgttgt
     7021 ccgaaggtgt ggtggcctct tcccgttatc cgaaacactg gatgaccgtg ggcgatccgg
     7081 cccgtgaatt caccatgacg cagtccgctc cgctgatggt gctgccggat ccggatgagt
     7141 ttgtggtggt acaggtgaaa taatccggga gcgggggcga aatgcccccg tgtctttttt
     7201 cacagggggc tgagatggca acaaaagaag aaaatctgaa tcgtcttcgt caactggctg
     7261 gtctgctggg gcgcgaggcg gatatgtcgg ggagtgctgc ggatattgct cagcgtgtgt
     7321 ctgagtggga agaggagctt gctgcttccc gggagggcat tatgcactct gatgagagcg
     7381 ggtctgatca aaatgacaca gacgatggtg agccgttgaa caacacggat gctccggatg
     7441 atgttaaagc cgtccgtgtg cgcaagtgcc tgcatgtgat ggggtattgc ccggagacag
     7501 gccgtcctgt tgagcttacg ttccggggga tgcgtgttct ggtgccatca tcactggcaa
     7561 tggccatgat acagcacgga acggctgagt atgcgtgatg ttcagaatgc ctttgatgcc
     7621 gcccttgtgg gggtggacag cacgattgtt gaagtgatgg ggatccgtgt gcagtttacc
     7681 tccggagcac aacgtggcgg tgaagttcag ggggtttttg acgatccgga gtcgctgggg
     7741 tttgccggtg gcggggtccg tattgaagga agcagcccgt cattatttgt gcggacggat
     7801 acggtgcgtg ccgtgcggcg tggtgacacg ctgaccatta acggcgagat gttctgggtg
     7861 gatcgtgttt ctccggatga cgggggcagc tgttatctct ggctcaaccg tgggcaacca
     7921 cccgctgtta accggcgacg ataaacgcag ggtgaaatta tggcgataaa agggcttgat
     7981 caggcgattg ataatctgag ccgggttcgt aaaaacgcca ttccggcggc ttcagcaatg
     8041 acgattaacc gcgtggccac aacgacgatt aatcagtctt catcacaggt tgcccgggag
     8101 acaaaggtac gccggaaact ggttaaggaa cggtccagac tgaaacgggc cacggtcaga
     8161 aatccgaatg ccagaattat cgttaaccgc ggtgatctcc ctgttattaa gctggggatc
     8221 agaatgctgg ggcgtcgtcc ggacagcata ctcaaagccg gtcagcatcg ttatcagcgg
     8281 gcatttatcc agcgattaaa taatgggcgc tggcatgtta tgcaacgtct tccccaggcc
     8341 agatatgaga agggcaatga cgacaagggc aggaaaaagc gtaatcgcct tcccattcag
     8401 gtggtgaaaa tcccgatggc ggccccactg aaacaggcgt ttgatgaaaa cgttgaccgt
     8461 atccgtcgtg aacgcctgcc caaagaactg gcatatgcgc tgaaacaaca actgaggatt
     8521 gcgataaaac gatgaaacat actgatatcc gtgcagccgt actggatgca ctggagaagc
     8581 atgacaccgg ggcgacgctg tttgatggtc gccccgctgt ttttgaggag gcggattttc
     8641 cggcggtcgc ggtttatctg acggatgcag agtataccgg tgaagagctg gatgctgata
     8701 cctggcgggc cacactgcat attgaggtgt ttttaccggc acaggtaccg gattcagagc
     8761 ttgatcagtg gatggaaagc cggatttacc cggcgatggc ggcgatccct gcactggcag
     8821 gcatgattac cacgatggtt acgcagggct atgagtatcg tcgtgatgac gatatggcat
     8881 tgtggagctc tgcagatctg acttattcca ttacatacga gatgtgagga tgatatggca
     8941 acaccaaatc ccctggcgcc ggtaaaaggt tccggcacca cgctctgggt ttacaccggt
     9001 aagggcgatg cttatgcaaa cccgctgtca gatgatgagt ggacgcgtct ggcaaaaata
     9061 aaggatctga cccccggcga gatgacggcg gaatcctacg acgataacta tctggatgat
     9121 gaggatgccg actgggtatc caccgggcag gggcagaaat ccgccggtga caccagtttt
     9181 acgctggcct ggaagcccgg cgagaaaggg cagcgtgatt tgattgcctg gtttgacagc
     9241 agtgagaccc gggcctacaa aatccgcttc ccgaacggta cggtggatgt gttccgtggc
     9301 tgggtgagcg ccattggtaa agcggtgacc gccaaagaag tgattacccg tacggtaaaa
     9361 atcaccaata tcggtcgtcc ttcgctggcg gaggatcggg gagaaatcac accggtcacc
     9421 ggtattaccg tgacgccact aacggggaat gtggcaaaag gtcagagcac caccctgaca
     9481 gtggccgttc agccggaagg cgcaacggat aaaacattcc gcgcaacgtc ggcaaatcag
     9541 aatttcgcca ccattaccgt gaaagggaac acgatcaccg tgaaaggtgt tgcggcaggt
     9601 aaagcgcaga tccctgttgt caccggcaac ggtgagtttg cggcggtggc ggagatcacc
     9661 gtcacggatg gcgctgcggg ctgaggggag gagatcaagc atgttcctga aaacagaaca
     9721 atttgaatat aacggtgtgt ccgtcacgct gtcggagctg tccgcgctgc agcggtttga
     9781 ttatatgaag tttgtttcag acgcagaaca acaggggaca acgaagcatg atgtcgtgca
     9841 cattaaccag cgatatctgg aaacggcatc cctgcttgtg gcgatgtcgc tatggcattc
     9901 ccattccctc aaaggcactc tggcctctcc ggagacagag atgcagcaga tccgccgtga
     9961 agtgatgctg ggatggcctg ctgatgcact gaatcaggca acgaaccggg tgctttatct
    10021 ttcaggtatg ctggataacc ggcacgatgc cgatcctgaa caaaccggga aagcagaagc
    10081 gactgagccg gtaacatcaa aaaagcattc gaaggcgagc tgaactttgt cctgaaactg
    10141 gcgcgagaga tggggagacc cgactggcgc gccatgcttg ccgggatgac atccaccgaa
    10201 tatgccgact ggcgacgttt ttactgcacg cattattttc aggataccca actggacgct
    10261 catttttccg ggctgatgta cgccgtactc agcctgtttt ttggcgatcc ggatatgcat
    10321 ccggcggatt tcagtctgct tgcgccagtg tgtgaggaag agcagacgga gatgccggac
    10381 gaggaagaca tgctgatgca gaaagcggca ggagttgccg gaggcgtccg gttctgtggg
    10441 gtgggtgggg gcgatatttc accttctgcg gatgtggtgg atgtcagcga agatgatgtt
    10501 gcattaatga tggcttcagc ggggatttcc ggaggtgtga gatatgtccc agccagcggg
    10561 tgatcttgtt attgatttga gtctggatgc ggcccggttt gatgaacaga tggcccgggt
    10621 acgccgccat ttttccagtc tggaggcgga tgccagaaaa accgccagta ctgttgaaca
    10681 ggggctgagc cgacaggcgc tggctgcaca aaaagccggg atatcagtcg gacagtataa
    10741 ggctgccatg cgcacactgc ccgcacagtt cacggatatt gtcactcagc ttgccggtgg
    10801 tcagaatccc ttccttatca tgctgcagca gggggggcag atcagcgatt cattcggtgg
    10861 accgctcagc ctgcttaccc tgctgaagga ggaacttctc gggatcaggg atgcctctga
    10921 atcatcagag gattcgctgt cagatacggc aaatgcactg gctgaaaatg cccggaatgc
    10981 cggtgaactg ggacgattta tgtcggtggc ccgtgtggcg gcaggtggcg gggttgccgt
    11041 actggccgcg cttgctgctg ccgcctggca ggcagagcag gctgaccggg ccttattgcg
    11101 ttcactgacc ctgaccggag gggcggctgc caccacaacg gcagaattgt ggaaaatggc
    11161 cggggtgatc agcgatgaag ccggtggtgg tatcagacag gcggcagaaa atctggcccg
    11221 tctggcagaa agcgggaaat ataccgccgg gcagctacgg atcatggggg aaacctctca
    11281 gagatggctg cagacggtgg gggacgatgc cgggaaggtg gaaaaagcct ttgaagggat
    11341 tgcagcagat ccggtgaagg cgctggcctc cctgaatcag cagtataact tcctgagcgt
    11401 ttcccagtta cgccatattg atgagcttga gcgcacgaaa ggtaaacagg ctgcggtgac
    11461 ggaggcgatg tccctgtttg cggatgtcat gaatgcacgt ctggagcaac ttgataaagc
    11521 ggccacgccg gtggaaaaaa tctgggacga tgttaaaacc tggacttctg acgcatgggc
    11581 atggataggt gatcatacac tgggggcact cagtctgatc actgacgtgg tggcaggaac
    11641 cgttgaacag gtgaagctgc tgcttgtgca gggggatctg gcgctggctg aatttattca
    11701 gtcagcctgg gaaacgacaa agaatgtgcc cggcgttggt gcgttgtttg gtgaactggc
    11761 agaagagaac cgcgtattta ttgagaaaac aaaacgcgat gaactggcgc tgagaaaatc
    11821 cattgcggaa cgggatgtgc gtatacgcca gggggaaatg gggtacatca accgttcgcg
    11881 tgcaacaggc gtcagcaaag gtccggggca gcaggaagcc gtcagccgtc tggctgaaga
    11941 gctgacaggt aaaaagcata catcaccgaa aacgcgctct gccggggaga gggaagagga
    12001 gcaggcaaga gaggctctgc ttgcccttga agctgagctc aggacgctgg aaaaacacag
    12061 cggtgcgaat gagaaaatca gccggcaacg ccgtgattta tggaaggcgg aaagtcagta
    12121 tgcggtcctg aaagaggctg ccacgaaacg acagttatcc gggcaggaaa aatccctgct
    12181 ggcgcataaa gacgagacgc tggagtacaa acgccagctg gctgagctgg gcgacaaggt
    12241 tgaataccag aaacgcctga atgagctggc acagcaggcg gtgcggtttg aagagcagca
    12301 gagcgcgaag caggccgcca tcagcgcaaa agcccgtggt ctcaccgacc gtcaggcaca
    12361 gcgggagtct gaagagcagc gtcttcgtga cgtgtacggc gataatccgc aggcgctggc
    12421 acaggtcacc ggggcactga aacagacatg ggcggatgaa gacatgctgc gcggtgactg
    12481 gctggccggg ctgaagtccg gctgggggga gtgggcggaa agtgcgacgg acagtttttc
    12541 gcaggttaaa agtgcggcca cgcagacctt tgatggtatt gcacagaata tggcggcgat
    12601 gctgaccggt gcagaggcag actggcgggg attcacccgt tcggtgctgt ccatgatgac
    12661 agaaatcctg cttaaacagg ccatggtggg aattgtcggg cgtatcggca gcgccattgg
    12721 tggtgctttc ggtggtggtg catctgcttc ctcggggacg gccattgagg ctgcggcggc
    12781 gaacttccat ttcgcgaccg gaggatttac ggggacgggc ggcaaatatg agcctgcggg
    12841 gatagttcac cgcggggagt ttgttttcac gaaagaggca accagccgga taggtgtggg
    12901 gaatctttac cgtctgatgc gcggctatgc ggaaggtggt tatgtgggtg gggccggaag
    12961 tccggcgcag atgcggcggg tggaaggtat taattttaat cagaacaatc acgtggtgat
    13021 tcagaacgac ggtataaacg gacaggcggg gccgcagctg atgaaggcgg tgtatgacat
    13081 ggcccgcaag ggggcgcagg atgaactccg gctgcagttg cgtgatggcg gtatgttatc
    13141 agggagcggg cgatgaaaac ctttcgctgg aaagtgaagc cggatatgga ggtgaactcg
    13201 cagccgtcgg tgcgtgaagt gcgttttggt gacgggtatt cgcagcgaac ggcggcaggg
    13261 ctgaatgctg acctgaagac gtacagggtg atgctttccg tgacccggga ggaggcccgg
    13321 catctggaag cgtttctggc agagcacggg ggctggaagg catttttgtg gaagccaccc
    13381 tatgcatacc ggcagataaa ggtgacctgt gccgggtggt ctgcgcgggt cgggatgttg
    13441 cgtgttgagt tcagcgcgga gtttaagcag gtggtgaact gatgcaggat attcacgaag
    13501 caagcctgaa cgagtcggtt aagtcagagc agtcaccgcg ggtggtgctc tgggaaatcg
    13561 acctgacggt gcagggcggt gagcggtatt ttttctgcaa tgagctgaat gaaaaagggg
    13621 agccggtgac ctggcagggg cgtgaatatc aggcgtaccc gattgagggc agcggctttg
    13681 agatgaacgg aaagggcagc agtgcccgcc cgtcgctgac ggtctccaat cttttcggcc
    13741 ttgtcaccgg gatggcggag gatttgcaga gcctggtggg tgccacggtg gtccgtcgcc
    13801 gggtgtatgc gcgttttctg gatgcggtga attttgtggc aggcaatcct gaggccgacc
    13861 cggagcagga gctgacggac cggtgggtgg tggagcagat gtcatcgctg acggccatga
    13921 cggcctcgtt tgtgctggcg acaccgacgg agacggacgg ggcgctgttt cccggtcgca
    13981 tcatgctggc gaacacctgt atgtgggatt accggggcga tgagtgcggg tataacggtc
    14041 cggcagtggc ggatgagttc gacaacccca ccacggatat ccggaaggac agatgcagta
    14101 aatgcatgcg cgggtgtgag atgcgcggca tggcggtcaa ttttggcggt ttcctttcca
    14161 tcaataaact ttcgcagtaa atcccgtttt atgacacaga ctgaatcagc gattctggtg
    14221 catgcccggc ggtgtgcgcc agcggagtcg tgcggcttcg tgataagcac cccggagggc
    14281 gaacggtacc agccctgcat gaatatctcc gcagagccgg aggcgtattt tcgtattgca
    14341 ccggaagact ggctgcaggc acagatgcag ggggagattg tggcgctggt ccacagtcat
    14401 cccggtggtc tgccctggct gagcgaggcg gaccggaggc tgcagataaa gagtgccctg
    14461 ccctggtggc tggtctgccg gggtgaaatt caccggttcc gctgtgtgcc gcacctgacc
    14521 ggacggcgct ttgaacacgg tgtgacggac tgttacaccc tgttccggga tgcataccat
    14581 ctggcgggga taacgctgcc ggattttgtg cgtgaggatg actggtggcg caacggccag
    14641 aacctgtacc tggacaacct ggcggaaaac ggcttttacc gggtgtctcc gtcctgtgca
    14701 caggcaggcg atatcctgct gtgctgcttt ggttcatcgg tgccgaatca tgccgccatt
    14761 tactgtggca acggtgaact gcttcaccat atacctgaac aactgagtaa acgggagagg
    14821 tattctgaaa aatggcaacg acgaacgcat tctgtctggc gtcaccgcca ctggtccgca
    14881 tctgccttca cggggattta caacgatttg gtcgccgcat cagcctgtat gtgaacacgg
    14941 cagcggaggc catccgtgcc ctgtcgctgc aggtgccggg attccgctgt cagatgaacg
    15001 aaggctggta tcagatacgt attgccggtg aggataccgc gccggaggcg gtgtatgcct
    15061 gtcttcacga acaactgggt gagggagcgg tcatccacat tgtgccgcga ctggccggag
    15121 ccgggggaaa tggtg
//
LOCUS       DAZGAP010000134        15110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGAP010000134 DAZGAP010000000
VERSION     DAZGAP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.135"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1331
                     /gene="chbC"
                     /locus_tag="ACHLAM_003627"
     CDS             <1..1331
                     /gene="chbC"
                     /locus_tag="ACHLAM_003627"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO7855093.1"
                     /translation="VLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFLS
                     FGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKV
                     DALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRN
                     WVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWTLNTWGTNFHQIIMDTISTPLAS
                     LGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAA
                     GKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEP
                     ILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNT
                     NGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1382..1732
                     /gene="chbA"
                     /locus_tag="ACHLAM_003628"
     CDS             1382..1732
                     /gene="chbA"
                     /locus_tag="ACHLAM_003628"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO7855094.1"
                     /translation="MMDLDNIPDTQTEVEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKTMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1740..2582
                     /gene="chbR"
                     /locus_tag="ACHLAM_003629"
     CDS             1740..2582
                     /gene="chbR"
                     /locus_tag="ACHLAM_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO7855095.1"
                     /translation="MMQPVINALEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2686..4038
                     /gene="celF"
                     /locus_tag="ACHLAM_003630"
                     /gene_synonym="chbF"
     CDS             2686..4038
                     /gene="celF"
                     /locus_tag="ACHLAM_003630"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO7855096.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4051..4809
                     /gene="chbG"
                     /locus_tag="ACHLAM_003631"
     CDS             4051..4809
                     /gene="chbG"
                     /locus_tag="ACHLAM_003631"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO7855097.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(5067..7328)
                     /gene="katE"
                     /locus_tag="ACHLAM_003632"
     CDS             complement(5067..7328)
                     /gene="katE"
                     /locus_tag="ACHLAM_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO7855098.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFILDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            7511..7774
                     /gene="cedA"
                     /locus_tag="ACHLAM_003633"
     CDS             7511..7774
                     /gene="cedA"
                     /locus_tag="ACHLAM_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO7855099.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7821..9212)
                     /gene="tcyP"
                     /locus_tag="ACHLAM_003634"
     CDS             complement(7821..9212)
                     /gene="tcyP"
                     /locus_tag="ACHLAM_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO7855100.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9345..9935)
                     /gene="ydjM"
                     /locus_tag="ACHLAM_003635"
     CDS             complement(9345..9935)
                     /gene="ydjM"
                     /locus_tag="ACHLAM_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7855101.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(10099..10767)
                     /gene="hxpB"
                     /locus_tag="ACHLAM_003636"
     CDS             complement(10099..10767)
                     /gene="hxpB"
                     /locus_tag="ACHLAM_003636"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO7855102.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYIDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10914..11450
                     /gene="yniB"
                     /locus_tag="ACHLAM_003637"
     CDS             10914..11450
                     /gene="yniB"
                     /locus_tag="ACHLAM_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO7855103.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(11491..12351)
                     /gene="yniA"
                     /locus_tag="ACHLAM_003638"
     CDS             complement(11491..12351)
                     /gene="yniA"
                     /locus_tag="ACHLAM_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO7855104.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12457..12747)
                     /gene="ghoS"
                     /locus_tag="ACHLAM_003639"
     CDS             complement(12457..12747)
                     /gene="ghoS"
                     /locus_tag="ACHLAM_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7855105.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            complement(12848..13777)
                     /gene="pfkB"
                     /locus_tag="ACHLAM_003640"
     CDS             complement(12848..13777)
                     /gene="pfkB"
                     /locus_tag="ACHLAM_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO7855106.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPVGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            14064..14822
                     /gene="ydiY"
                     /locus_tag="ACHLAM_003641"
     CDS             14064..14822
                     /gene="ydiY"
                     /locus_tag="ACHLAM_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO7855107.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(14875..14982)
                     /gene="yniD"
                     /locus_tag="ACHLAM_003642"
     CDS             complement(14875..14982)
                     /gene="yniD"
                     /locus_tag="ACHLAM_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO7855108.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         3798 a         3560 c         3838 g         3914 t
ORIGIN      
        1 aggtactcct cccttttgca gttaaaatag gaaagcagcc acacgttaat gcaatcaaaa
       61 atggctttat tcgcttaatg ccgttaaccc ttgcgggggc catgtttgta ttaattaaca
      121 acgtttttct aagctttggg gaggggtcgt ttttttattc cttaggtatt cgcctggatg
      181 cctcaaccat tgaaacactt aatggtctga aaggtattgg cggcaacgta tataacggaa
      241 cattagggat aatgtcttta atggcaccgt tctttattgg catggcgctg gcagaagagc
      301 gtaaagtcga tgcgctggcg gccgggttgt tatccgttgc agcatttatg accgtcaccc
      361 catatagtgt cggtgaggcc tatgcggttg gcgcaaactg gttaggtggg gcgaatatca
      421 tctccgggat tattattggc ctggtggtgg cagaaatgtt tacctttatt gtccgccgca
      481 attgggtcat taaattgccc gatagcgtac ctgcttcagt atcgcgttcc ttctcggctt
      541 taattcccgg ctttattatt ctttccgtga tggggattat tgcctggacg ttgaatacct
      601 ggggcaccaa cttccatcag attattatgg ataccatctc aaccccactg gcatcgttgg
      661 gtagcgtggt gggctgggcc tatgtgatct ttgttccact gctctggttc ttcggtattc
      721 atggtgcgct ggcgctgacc gcactggaca acggcattat gacgccttgg gcgctggaaa
      781 atatcgcgac ctatcagcaa tatggttccg tcgaagcggc gctggcagcc ggtaagacct
      841 tccatatctg ggccaagccg atgctggact cctttatttt ccttgggggc agtggtgcga
      901 ctttaggtct gatcctggct atctttatcg cctctcgccg tgctgattat cgtcaggtgg
      961 caaaactggc gctgccgtcc ggcatcttcc agattaacga accgattctg tttggtctgc
     1021 caattatcat gaacccggtg atgtttatcc cgtttgtact ggtacaaccg attctggcgg
     1081 caatcaccct cgcagcgtac tacatgggca ttattcctcc ggtgaccaat attgcaccgt
     1141 ggaccatgcc aaccggtctg ggagccttct ttaacaccaa cggtagcgtc gccgcattgc
     1201 tggtcgcact cttcaacctt ggtatcgcaa cgttaattta tctgcccttt gttgtggtgg
     1261 ctaacaaagc acaaaacgcg attgataaag aagagagcga agaagatatc gctaacgccc
     1321 tgaaatttta atcgatgccg gtacgggtaa tcgtgccggt aagaatagag aggaacgatg
     1381 tatgatggat ctcgataata ttcccgatac gcaaacggaa gttgaagagc tggaagaagt
     1441 ggtgatgggg cttatcatca actccggaca agcgcgcagc ctggcgtatg cggcactgaa
     1501 acaggcgaag cagggcgatt ttgccgcagc aaaaaccatg atggatcagt cacggatggc
     1561 attgaatgaa gcgcatctgg tacagacgaa actgattgaa ggcgatgcgg gcgaaggtaa
     1621 gatgaaagtg agtctggtgc tggtccacgc tcaggatcat ttaatgacgt ccatgcttgc
     1681 gcgtgaactg attactgaat taattgagct tcatgaaaaa ctgaaggcat aaggagtcga
     1741 tgatgcagcc agtgattaac gcgctggaaa ttgccactgc ccgagaacag cagttgttta
     1801 atggcaaaaa tttccatgtg tttatctaca ataaaactga gagtatcagc ggactgcatc
     1861 agcacgacta ttatgaattt actctggtat taaccgggcg ttatttccag gagattaacg
     1921 gtaagcgcgt gttactggaa cggggcgatt ttgtttttat tccgttaggt tcgcaccatc
     1981 aaagttttta tgaatttggt gccacgcgta tattgaacgt tgggatcagt aaacgctttt
     2041 ttgagcagca ttacctgccg ttgttgcctt attgctttgt cgcttcgcag gtataccgga
     2101 ccaataacgc ttttctcacc tatgtggaaa cagtgatttc ttcattgaat ttccgcgaaa
     2161 cagggctgga agagtttgtt gagatggtta ctttttatgt cattaaccgt ttacgtcatt
     2221 accgcgaaga acaggtgatt gatgatatac cgcagtggct gaaaagtacg gtagaaaaga
     2281 tgcatgataa agagcagttt agtgaatcgg cgctggagaa tatggtgacg ttgtcagcca
     2341 aatcacagga atatttgacg cgagcgactc aacgatacta tggtaaaacg ccaatgcaga
     2401 ttattaatga aatccgtatt aattttgcca aaaaacaact ggaaatgacc aactattcag
     2461 tgacggatat tgcgtttgag gccggttata gtagcccgag cttgtttatt aaaacgttta
     2521 agaaattaac atcctttacg cctaagagct atcgtaagaa attgactgaa tttaatcagt
     2581 aagtcgttat acctgacaat tcacatatct gtcctgttgc tggattattt atgtccgggg
     2641 ggcagatatg ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta
     2701 aaagtcgtca ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt
     2761 aagcgttatc acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga aggtggtaaa
     2821 gcgaaactgg atattatttt tgatctctgc caacggatga ttgataacgc tggcgtcccg
     2881 atgaagcttt ataaaacgct ggatcgccgc gaagcattga aagatgctga tttcgttact
     2941 acccaactgc gcgttggcca attaccggcg cgcgaactgg atgaacgtat tccattaagt
     3001 catggttatc ttggtcagga aactaacggt gcgggcggtc tgtttaaagg tctgcgtacc
     3061 attccggtga tttttgacat cgtaaaagat gtcgaagaac tatgtccgaa tgcatgggtg
     3121 attaacttca ctaacccggc gggaatggtc actgaagccg tttatcgtca taccggattt
     3181 aaacgcttta tcggcgtgtg taatattccg atcggcatga agatgtttat tcgcgatgtt
     3241 ctgatgctga aagacagcga tgatttatct atcgatctgt tcggcctcaa ccatatggtg
     3301 ttcattaagg atgtgctggt aaatggcaaa tcacgctttg ccgaattgct tgatggtgtg
     3361 gcatccgggc agttaaaagc atctggcgtt aaaaatattt tcgatctgcc atttagcgaa
     3421 ggcttaattc gttctatgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa
     3481 aaagagatgc tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta
     3541 gtacagaaag tcgagaaaca actttttgag ctatataaaa acccggagtt gaaagttaag
     3601 ccgaaagaac tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc
     3661 aacgctatct acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat
     3721 attgataata ttccggcaga ctgggcggta gaaatgacct gtacgctggg gcgcgatggc
     3781 gcgacgccac atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc
     3841 attaaaggct tcgagattgc tgccagcaac gccgcactta gcggagagtt taacgatgtg
     3901 ttactggccc taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca
     3961 cgcgagatga ttctggcgca cgagaaatgg ttgccaaatt ttgccgactg catcgcagag
     4021 cttaaaaaag cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat
     4081 gattttggct taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt
     4141 gtcacatcga cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt
     4201 cttgatgaac cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg
     4261 acagctatgc cggggttaac tcgcgatggt gtgctgggaa aatggatctg gcagttggca
     4321 gaagaagatg ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt
     4381 ttcattgagt tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg
     4441 ttcccgcaga ttttcccgat tgtggcaaag tttgcggctg aagaggggat tgcgttgcgc
     4501 atcgaccgtc agccgctatc taacgatggt gatttaccgg ctaatctgcg cagttcgcag
     4561 ggattcagta gcgcgtttta tggtgaagag attagcgaga cactgttcct acaagtgctt
     4621 gatgactcca gtcatcgcgg cgagcgctct ctggaagtga tgtgccaccc ggcgtttatc
     4681 gataatacga ttcgccagag tgcttattgc ttcccgcggt taacggagct ggatgtgttg
     4741 acatcagcgt cgttgaaagg tgctatcgct cagcgtggtt atcggttggg gtgttatcgg
     4801 gatgtgtaag tgaggatgtt tgccagatgc ggcgtgaacc ttgcatccgg cgattaattc
     4861 ctacaaaggc ggcgtaactg cgccgcttga gactgctgac aaacgcaaaa ctgcctgatg
     4921 cgcttcgctt atcaggccta cgtttttcct gcaatatatt gaatttgcac agttttgtag
     4981 gccggataag gcgttcacgc cgcatccgac ataaacaaag cgcactatgt aaatcatttg
     5041 aggcggcgca actgcgccgc ctcccatcag gcaggaattt tgtcaatctt aggaatacgt
     5101 gaccacacgc ggtgtgctgc catcagcgtt aacagttcat ccataaaact accgtcggcg
     5161 ctgtcagctt ccacaatccc ttcttcaccc tggtcagcga ccttgattgt tgctttaaac
     5221 ttgcgcgcgt ctcctgccag cgcaatcggt ttaaggtgtt tgtaggcttc catcaggtag
     5281 tagttggcat cgccgttgtc agcgatatcc gcgatattgc cgcaagggac aataaccgca
     5341 tcgaccgtca gcgaaggtgc accggcaaag gtagcggcta taggcaacac cgtaccgtca
     5401 tccgcagtca cttcacccat tcgggagtag agcagtttgg catgaacgcc tttggccttc
     5461 agcgccttga gaatggccag aaggtctgcc gatctcactt catcattaag taaaatcgct
     5521 accacgcgac ctttcacatc accgtcagga atggcgtaca aacttaagga tggatccttt
     5581 ttcagaccgt tgacgtccgg aggcggggtg atattcagct ggtcgtccgt cagctcgata
     5641 ccgagatttt tcgccaccgc ctgggccaga gtgagatcaa tatgtgccag ctggtcaaca
     5701 acgcgctcac gaatatacgg acgaacgact ttgcttaact caaaactgaa accatcgaca
     5761 atatggcgct gctcgaatgg cgtctgactt agccagaaca gacgcggatg ggaataatat
     5821 tcgccaaacg atgggctgcg ctcgcgaact ttattgcctt ccacgcgctc ctggtatgat
     5881 tcaaaaccgc cgcgtttcgg ccccggcggt gtttcgcgcg gccagttatc gttgatcgag
     5941 ttcggttcat aattcgccgg gttagtgtcg atccccatac gatgcatgcc gtcacgctgg
     6001 aaattatggt aagggcaggt cgggcggtta atcggaatct catggaaatt cggcccacca
     6061 agacgactga tttgtgtatc ggtataggag aacaaacgtc cctgcaacag gggatcgttg
     6121 gtgaagtcca gaccaggaac aatatgacct ggatggaatg ctgcttgttc gttttcggca
     6181 aagaagttat ccggattgcg attgagcacc atcttgccga cacgctgaac gggcaccagt
     6241 tcttccggga taagtttagt tggatcaaga aggtcgaagt cgaacttaaa ttcatcttcc
     6301 tcaggaatca actggaagcc cagttcgtat tccggaaaat cgcctgcttc gatggcttcc
     6361 cacaactcgc ggcggtggaa gtccgggtca cgtccggtta gtttttgtgc ttcatcccaa
     6421 acgagtgagg ctttacctgc cagtggtttc cagtggaaac gtacgaacgt tgccttccct
     6481 tcggcattaa tcaggcggaa ggtgtgaata ccgaagcctt ccatggtgcg gtaactgcgc
     6541 gggatcccgc gatccgacat cgcccacatc acgttgtgca gagtttcagg ttgcagagaa
     6601 acataatccc agaaagtatc gtgggcgctt tgcccttgtg gaattgccca gtgcggttct
     6661 ggttttaccg catgaacaaa atcggggaat ttatgcgcat ccaggataaa gaagattggc
     6721 gtgttattgc caacgaggtc aaaaataccc tcttcggtat agaacttggt ggcaaagcca
     6781 cggatatcac gcacggtatc ggcagagcca gcaccaccct gaacggtaga gaaacgtaca
     6841 aatactgggg tgattttgtt cggatctgag aggaaatctg ctttggtgat atcgcttaag
     6901 cttttatatg gctggaaata accgtgagct gctgatccgc gtgcatgaac aatacgttcc
     6961 gggatgcgct catggtcaaa gtgagtgatt ttctcgcgca gaataaaatc ttccagcaga
     7021 gttggaccac ggctaccggc acgcagtgag ttttgatcgt cggcgatgcg cacgccctga
     7081 ttagtggtca gcgcataatt ttcactgcct ttgcgtacgt cttccagaga attaagtttt
     7141 tcgttacgcg tatcaggggc tttcaggctc cctggggcgg taggttgtgc accaggcggt
     7201 gttggttcag ccgctggacg atgagatccg tcttcaggtg ccagtgagtc catccccggt
     7261 ttcgcttcgc tggaatcgtg tagtggtgac tggtgctgat gtgggttctt ttcgttatgt
     7321 tgcgacattg aactcgtctc cttaaattat tactgaaagg gccgctgttt tggtcaaaaa
     7381 accctctaac tatagaccat cagccagatc ccgcttcgga gactattctg gaaacggtgt
     7441 tatgaaatac acgcaaagcg ggacgtacag gggctaaatc ggctaaacta cagtttttct
     7501 gatttttaat atgcgtttag tgaagccagt aatgaagaaa ccgctccgcc agcaaaaccg
     7561 ccagattatt agctatgtcc cacgcacgga acccgcgccg ccagaacatg cgataaagat
     7621 ggattcgttt cgtgatgtct ggatgctgcg tggcaaatat gttgcgtttg tactgatggg
     7681 agagtcattt ctgcgctcac cggcgtttac tgtgcctgaa tccgcccagc gttgggcaaa
     7741 tcagattcgt caggaaaatg aggtcgaaga ataaaaaaac acctgtcggg atgacaggcg
     7801 tttaacgtaa caaatcagga ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga
     7861 atagctttat cggtttgctt cagccactgg ctggtcagcg tgccagcggt catcgagcca
     7921 ctaacgttta gcgccgtacg acccatgtcg ataagcggtt caacggagat taacaatgcc
     7981 accagcgtta ccggcaggcc catcgcaggc agcacaatca gtgcggcgaa agttgcacca
     8041 ccaccgacac cggcaacgcc tgcggaacta acggtaacaa taccgaccag cgtcgcaatc
     8101 cacatcgggt ccatcgggtt aatgccaacc gtaggcgcaa ccatcaccgc cagcatcgcc
     8161 ggatacaaac cggcacagcc gttctgacca atggttgcac cgaaagaggc ggcgaaactg
     8221 gcgatggatt caggaacacc gagtcgacgc gtttgtgctt ccacattcag tgggatagac
     8281 gcagcactgg aacggctggt aaaggcaaac gtcagtactg gccatacctt acggaagtac
     8341 ttcagcgggc tcacgccatt aatacccagc agaaggccat gcactgcaaa cataatcaga
     8401 agaccgaggt aggacgcgac aacgaaactt cccagtttga tgatgtcttg caggttagaa
     8461 cctgcaacca ctttggtcat cagcgccaga acgccgtaag gggtcagctg catgaccagg
     8521 cgaaccagtt tcatcaccca gctttgcagg gtatcgatag cggttaagac gcgttcacct
     8581 ttcggcgcat catccttcag cagcttcagc gcagctacgc cgaggaatgc ggcaaaaatt
     8641 accacgctga taattgacgt cggattggct ccggtcaaat cggcaaacgg gtttttcggg
     8701 ataaaggaca agaccagctg cggaacgctc aggtcagaga ctttaccaac atagttactt
     8761 tcgatggcgt tcaaacgtgc agtttctgca ccaccctgaa ccagaccttc agccgtcaaa
     8821 ccaaacaggt tggtgaccag cacaccgacc agcgccgcaa tcagtgtggt aaacaaaagc
     8881 gttccgatgg tcagaaaact gattttgcct aactgtgatg cgttatgcag acgggcaacc
     8941 gcgctcagaa tagaggcaaa gactaacggc ataacgatca tttgcaggag ttgaacatag
     9001 ccgttaccaa caatgttaaa ccactgtaca gaatctttaa gtacctggct gtcagaacca
     9061 taaatggtat gcagggcgag gccaaaaacc acacccatca ccagacccac cagcactttt
     9121 ttcgccagac tccactgttt atggcgggtc tgagccagcg caaacagcag tacaacgaac
     9181 accacgatgt tcgcaattaa tggaaagttc atccccgttc tcctgatctt attatttcta
     9241 tcactgcgta gcagtcatag tgttggcgca agattaacag aactgtgagc tggctcttat
     9301 attcaaatgg aattgcttat tccagaaagg tgatttttgc ctttttagta ttcaacctga
     9361 tgcttaataa gccgatgaaa ctggatctgc aaggtattga tcatttgcga tgaccaacgg
     9421 acagcgctgt tttcgggtaa tgaatggggc atccataccg accagacaaa taatgccatg
     9481 cagataaaac gttccagttg gttgcccttt tggggaacca ggataggcaa gcggaaacgc
     9541 cagcggcatg gccagagcag gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg
     9601 ctcaaataac ccagcaccat tccttgtagc gcatcagccg gaatgaacca gctttcagga
     9661 accttaaggt aaaaggttgc cagcagcgca aataccgcca gcagactgtg ggtaaaacca
     9721 cgatgcccaa aagcgcgggc gattggtttt gatatccact ttaatcgctg cccaagaaac
     9781 gactttgggt gatcgatgtc cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc
     9841 caccagtcac cctgtgccag cacgggcgtc agctcggcat ttttggcaaa taccgcacaa
     9901 gcaatagaaa agagaaggtg accttccgcc gtcatgataa aactcactaa actgtcgata
     9961 cgtacagtat agggttttta tacagtgcac ggaagtggtg acggtgtaac tctttctctt
    10021 aaattggcga ttaatccgtc agttgaatgc aaattcagat aaagaaccag gggcagtgtg
    10081 atcactgccc cctggtcatt aaccgagaag gtcttttgcg gtgagttctg tcagcgatga
    10141 gagtttgacg tttgctaata caaaacgtgg atcattttgc gcttctggcg caggaacgac
    10201 gatggaacgc atgcgggctg ctttagaggc gatcatgcca tttaccgaat cttccagtgc
    10261 cacgcaggtc agagggtcaa cccccagttt tgctgcgcag tcgatatata cttgcggatg
    10321 cggcttgctg taaggcaatt tttcagccga ggcgagggca tcgaaactgt cgcgtaaatc
    10381 aaacatggtc aatacttttt ccagcatatg cagcggtgac gcggaggcca gccccaccaa
    10441 taaaccttgt tctttgcata acgcaacggc ttcacgcaca cctggtaata atggacgcgt
    10501 ctcttcaacc agtgaaatgg cacgagcaat aacttgttct actacttcct gacggcttgg
    10561 cccattccat ggttgccggg cgtaccaaag atcgaccacc atatcgatgc gtaaacctaa
    10621 ggtgtccggc agctcgttac gacgggagat atccaccccc aggcttgcca tcacatccag
    10681 ttcggctcga tcccataaag gttctgagtc gataagtaat ccatccatat caaaaattgc
    10741 agcaagaatc tgacgcgggg ttgacattac aacctctcct ttagagcatt atatgtaagg
    10801 tattaagccg tgcttatgat taagcgccta tcatatcgcc tttaaagaat gggcgaaaac
    10861 agatatctac aggtaaacta agtgaaacga cgcgtcttaa tcaaggagaa ctcatgacgt
    10921 atcaacaagc tggacgcatt gctgttttga aaaggatttt gggttgggtg atttttatac
    10981 ctgcattgat ctctacattg atttctttgc ttaaatttat gaatacccgg caggaaaacc
    11041 aggaaggcat taatgcggtc atgctcgact ttactcatgt catgatcgat atgatgcagg
    11101 cgaatacgcc ttttttgaat ctgttctggt ataactcccc gacgcccaat tttaatggtg
    11161 gcgtgaacgt tatgttctgg gtgattttct tcctgatttt tgtcggactg gcgttgcagg
    11221 attccggtgc ccggatgagt cgccaggcgc gcttcttgcg ggaaggtgtt gaagatcaac
    11281 tcattctgga aaaagccaaa ggggaagagg ggctaacgcg tgaacaaatt gagtcacgta
    11341 ttgttgtgcc gcaccatact attttcctgc agtttttctc gctgtatatc ctgccggtta
    11401 tttgtattgc tgcaggttat gtgttctttt ctctgctggg gtttatttaa tcaaaaggcc
    11461 gcacggagcg gccattatcc tcaccccata ttatgctgct aataatctat ccagtgactg
    11521 ctgagcaata accaaatgct gaccgccaaa taaccttgca cgatttaaca gcgtgtagag
    11581 ttggtaaacc ggttgacgtt caaggaaatc ggcaggtagc ggggatactg actgatagcc
    11641 gtcatagatt tgtggcggct gttcagtatg cagcggtaac atcgccaggt cgcactctcg
    11701 gtcaccccag tagcaggccg ggtcgaaaat gtacgggcca tccggaccca gcgcacagtt
    11761 gccggaccat aaatcgccgt gcaacagaga aggctgcggt tgatgcgagg ccagacgctg
    11821 ctgaatatgc tcgacgatgg catcgatatt gccgaaagcg atcccttttt ctgccgccag
    11881 ttccagctgc cagccaatcc gttgttcagc aaaaaaagtt gaccagcgac gttgccaggt
    11941 gttgggctgg ggagttgtgg agagcgcgtt atcgaaatcg aggccaaatt gcggttggtc
    12001 gctccattga tgtaaacgcg caatttgctg accaagaata aatgcgctat gcgcatccag
    12061 cgggcgaggt gggagataat ccatcaccag aaaactgtag tcacggtcag cgccaactgc
    12121 ccaaacctta ggcacggtga cggttttact acgcgacagt aactccagtt ggtcggcttc
    12181 ggcggtaaaa ccgggaagca gttccctttc atcacatttg acgaaaaagt catgtcctgc
    12241 atagcgcaaa tgccatgcgg catggacttc tccgccaggc agttcattac gcagttcgat
    12301 ttcgccttca cctaactgct cgctcaaaag acgactgatt gcctgccaca tgatgtctct
    12361 cccatgttgt ctgccagatc ataaggttag cgcaagaatg cggtgtaaaa acacgagcta
    12421 acgcacacca cagactgatt attgcccaaa aaccatttat tttctgttgc tattccattc
    12481 ctcccaggtc gtgatggcta tttcaggatc tttaccggtt gcactttggg taagccccga
    12541 aacaagttct ctaatttctt cttcactttg ggtactgata agtccaaaag tattagtccc
    12601 cagctcatgg atatttccat catcatccgt catggtgagc aaaaaaccgt cgcgagtcag
    12661 gtaattattc aactcgttaa tttcagtcaa tgtatcttcg tgcaacatga cggttatgac
    12721 ataacggaca agatcgccac tggccatagt tcaccccttt gttatcacat gacttttgag
    12781 catagtcgga gaaacgcgtt gccgacaggt tggtggtgat tcccccaatg ctgggggaat
    12841 gtttcagtta gcgggaaagg taagcgtaaa ttttttgtgt atcgtcatgg gagcacaggc
    12901 gtgttccctg attgagtgtg gctgcactcc ccgcagctac gccaaaacgg accatctctt
    12961 caagagaggc attttctgcc agtttcagtg tcatcgcgcc gaccatgctg tcaccagcgc
    13021 caacggtgct ctggcttttc accggtggtg gcacaacctg aatgcagttt tcactatcaa
    13081 cgcccagcgc cccttgtgga cccagggaaa cgacaactcg tttggccttg ccgctattaa
    13141 cgatttcctg cgcggctttg cggacatcgt ctggctgggt gagttcgcga ttcaccagcg
    13201 cactgagttc tttttggtta ggcttaacca actcgatgtt accaatcgcc agtgctgcac
    13261 ttaacgcttc gccagaactg tcgacgatac agcggatccc ttggttttgc gcagcggaaa
    13321 tcagttgggt taatttttcc agcttcacac ctggcggcag gcttccgctt atgaccagga
    13381 tagcaccgga ttcaatttcc agaacttgct cttcaagctg gcgaaactca tcttcattta
    13441 atgccgcgcc tggcatgaca aaacgatact gctcaccgct tgcttccaca tgtacgtgta
    13501 aattctgccg ggtccagtct ttggcttcta cagtagcgac ggggacattt tcatctgcca
    13561 acagtgaaac cagatgttcg ccggtcgcac ctcccaccgg gaagatcgct gtagcactgc
    13621 ctccaagatg ggcaatggcg cgggcgacgt tgatgccgcc gcctccgggt tcgaataccg
    13681 gtgcggtaca gcgcagcttt ccttcgggat aaatttgcgg ggtaattgtt gcgctatcga
    13741 gagagggcgc aagtgtcaac gtatagatac gtaccatcat ttcctcctat aggctgattt
    13801 cagtctggca ccgaatcagc ggaaagtgaa gaaattaaca atatgattta ttagagcttt
    13861 atttaaaatt ttgcagataa atatatataa ataaaaattt gttttcgcca catgcttatt
    13921 caaaaaatga aataagaata cattgctaag ttattcgcgc ctttttataa ttcgttacct
    13981 ttccagggac gctaatcttt gatccttaag aaagtttccg ggaccgcgaa ggtctctttt
    14041 tttgttgtac ggactcttga taaatgaagc ttttgaagac agttcctgcc atcgttatgt
    14101 tggcgggggg catgtttgcg tcactgaatg cagctgccga tgattccgtt tttactgtca
    14161 tggacgatcc tgccagcgct aaaaaacctt tcgaaggtaa tctgaacgca ggatatcttg
    14221 cacaatcagg caatacgaaa agctcctcac ttacggctga caccaccatg acctggtatg
    14281 gacaaaccac tgcctggtcg ctgtggggga acgccagtaa tacttcttct aatgatgagc
    14341 gttcttcaga aaaatatgcg gcgggcgggc gtagccgttt taatttaact gattatgact
    14401 atttatttgg gcaggcaagc tggctgacag accgttataa cggctatcga gagcgcgatg
    14461 tgttgaccgc gggttatggt cgccagtttt tgaatggccc ggtacacagt ttccgctttg
    14521 aattcggtcc aggtgtgcgt tacgacaaat ataccgataa tgccagtgaa acccagccgc
    14581 tgggttatgc ctccggggct tatgcatggc agttgactga caacgctaaa tttacgcagg
    14641 gtgtttccgt ctttggcgcg gaagacacaa cgctgaactc tgaaagcgca ttaaatgttg
    14701 ctattaatga acattttgga ctgaaggtgg cttacaacgt aacatggaac tctgaacctc
    14761 cagagtctgc accggaacat accgatcgtc ggacaacctt atcgctgggc tacagcatgt
    14821 aaaattatct gccggaccgt gttatgcggt ctggctgtct tcgtttgtca ctgctcagcc
    14881 ccaaatcatt gctgcccaac gtagcaacaa catagcgcca cagattgcca gtagcaccac
    14941 caccatcttc cagtgttttt tatcaaagcg tttagtcggc ataccagtgt cctgcatatc
    15001 tcttgtgcgt gataacggag tgtaacaaaa gggacaggca tcaagggatt ttttgttatt
    15061 acagccgtcg ggtggcgttg tacacctgat cactgttttt gcggtttatg
//
LOCUS       DAZGAP010000135        15061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGAP010000135 DAZGAP010000000
VERSION     DAZGAP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.136"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(277..1086)
                     /locus_tag="ACHLAM_003643"
     CDS             complement(277..1086)
                     /locus_tag="ACHLAM_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFO7855109.1"
                     /translation="MTNIFTLMNPISSATETLLSQINRICTAQGIPFFIAGATAREIL
                     LHHIHSRSIGRRTRDIDIAVFVDSWPRFDALKNAFLAEGAEVVQGNAHRMIVKGIELD
                     IIPFGGVAEGNQIAWPPEREIIMAVDGFAEVFSHTVVVRLDNSENIPFCSLPGLALLK
                     LFAWRDRGHGSTKDATDLFKIICEYGVIENERIYESPVEGERLNWDPIRMGAFLLGSD
                     IATISEHSSLQDLLHLDKERLIDVIVRQHDVGDAGIEQIINDFWDGMASDG"
     gene            complement(1040..2038)
                     /locus_tag="ACHLAM_003644"
     CDS             complement(1040..2038)
                     /locus_tag="ACHLAM_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFO7855110.1"
                     /translation="MKEEQLLSDAIENLPKGFHLQYEHSGRHGKHDAWGKLTGPDGES
                     VIFALEVKFIHRKESLMAVREQMVRFPADFPVLLVCNRLTSALAEYCADSQINFIDTA
                     GNARIQVPGLYVRIEGRYEKKPVSASSRFAEGVMKLLFVLLSSPETLNETYRSLAGKA
                     GISLGMVSKAFDYLEAQRYFRKSKKGRRLINEGELEALWLKDYATALRPKLDSLSLSA
                     PVSWDELTLASGEYWSGEIAAAKLSGGYLIPENGVIFTSHPLLQRRKELGLKPVPGGK
                     LQLIAGFWGNFVLNHKAEAMLCVAELLASGDDRNWETARIINDKYLHLNESDLFSY"
     gene            complement(2666..3913)
                     /locus_tag="ACHLAM_003645"
                     /pseudo
     CDS             complement(2666..3913)
                     /locus_tag="ACHLAM_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4136..4220)
                     /locus_tag="ACHLAM_003646"
     tRNA            complement(4136..4220)
                     /locus_tag="ACHLAM_003646"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4184..4186),aa:Leu,seq:caa)
     gene            4416..5435
                     /gene="ahr"
                     /locus_tag="ACHLAM_003647"
     CDS             4416..5435
                     /gene="ahr"
                     /locus_tag="ACHLAM_003647"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO7855111.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            5565..7067
                     /gene="yjgR"
                     /locus_tag="ACHLAM_003648"
     CDS             5565..7067
                     /gene="yjgR"
                     /locus_tag="ACHLAM_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO7855112.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLPWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(7186..8268)
                     /gene="lptG"
                     /locus_tag="ACHLAM_003649"
     CDS             complement(7186..8268)
                     /gene="lptG"
                     /locus_tag="ACHLAM_003649"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7855113.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8268..9368)
                     /gene="lptF"
                     /locus_tag="ACHLAM_003650"
     CDS             complement(8268..9368)
                     /gene="lptF"
                     /locus_tag="ACHLAM_003650"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7855114.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(9431..9481)
                     /gene="ytgA"
                     /locus_tag="ACHLAM_003651"
     CDS             complement(9431..9481)
                     /gene="ytgA"
                     /locus_tag="ACHLAM_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO7855115.1"
                     /translation="MSIIILATISTTQENK"
     gene            9635..11146
                     /gene="pepA"
                     /locus_tag="ACHLAM_003652"
     CDS             9635..11146
                     /gene="pepA"
                     /locus_tag="ACHLAM_003652"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO7855116.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYRGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            11280..11723
                     /gene="holC"
                     /locus_tag="ACHLAM_003653"
     CDS             11280..11723
                     /gene="holC"
                     /locus_tag="ACHLAM_003653"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO7855117.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            11723..14578
                     /gene="valS"
                     /locus_tag="ACHLAM_003654"
     CDS             11723..14578
                     /gene="valS"
                     /locus_tag="ACHLAM_003654"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO7855118.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESTQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLSADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(14632..>15061)
                     /locus_tag="ACHLAM_003655"
     CDS             complement(14632..>15061)
                     /locus_tag="ACHLAM_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFO7855119.1"
                     /translation="DSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVS
                     LRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQA
                     KNTYLLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3740 a         3964 c         3928 g         3429 t
ORIGIN      
        1 atagccataa tattagcgca tcatgccgaa ggtgcggtgc gccgtggaag gtggaacatc
       61 aagtttacct gcgttctcca ctatctttga tgggaaccag caagcctttg accttcagtt
      121 tgatgatgtc gcgactgact ctgggctggc attattgata acataagcgc atcttaatcg
      181 ggcagttggc gttacgccct gccattgttc acaataatac cgttcatgat tcatgaacaa
      241 aactctgttg tgaacatttc atctaaaact acatcctcaa ccatcacttg ccataccatc
      301 ccagaaatca ttgatgatct gctcaatccc agcatcaccc acgtcatgtt gtcgtactat
      361 cacatcgatc aggcgttctt tatcaagatg gagtaaatcc tgcaaggatg agtgttcact
      421 aattgtcgct atatcgcttc ccaacagaaa agcgcccata cgtatgggat cccagttcag
      481 gcgttcccct tcgacgggcg attcatagat gcgttcattc tcgataacac catattcaca
      541 gataatctta aagagatcgg tcgcgtcttt agtactccca tgcccacgat cacgccatgc
      601 aaaaagtttt aacagagcca gtcctggcaa ggagcagaac ggaatatttt cgctgttatc
      661 cagccgcaca actacggtat gagaaaagac ttcggcaaat ccatccacag ccattatgat
      721 ctcacgttct ggtggccagg caatttgatt tccttcagca acaccaccaa aaggaataat
      781 atctagctcg atccctttga caatcatacg atgggcatta ccttggacaa cttctgcccc
      841 ttcagcaagg aaagcatttt taagcgcatc aaatctcggc cagctatcca caaagacggc
      901 aatatcaata tcacgggttc ggcgaccaat acttctgctg tgtatatgat ggagcagaat
      961 ttcacgagcc gttgcacctg caataaaaaa cgggatcccc tgagcggtgc aaatacggtt
     1021 gatctgactc agtaacgttt cagtagctga agagatcgga ttcattaagg tgaagatatt
     1081 tgtcattgat tatccttgct gtttcccagt ttctgtcatc cccgcttgcc agcagttcag
     1141 ccacgcatag catcgcctcg gctttgtgat tcagtacaaa gttcccccaa aaaccggcga
     1201 tcagctgtag tttccccccc ggaacaggtt ttagtcccag ttcctttcgc ctttgtaaca
     1261 acggatggga tgtaaaaatc acgccatttt ctggtatcag gtatcctccg ctcagctttg
     1321 ccgccgcaat ctcgccgctc cagtattccc cggacgcaag cgtcaactca tcccatgaaa
     1381 caggcgcgga taaagaaagc gaatccagct tagggcgcaa agctgtagca taatctttca
     1441 gccacaaagc ctctaactcg ccttcattga ttaagcgacg ccccttcttc gatttgcgga
     1501 aatagcgttg cgcttcgaga taatcaaaag ccttgctgac cattcccaga gagatacctg
     1561 cctttccggc cagactgcgg taagtctcgt tcagggtctc tgggctagac agcaaaacaa
     1621 aaagaagctt catcacccct tcagcaaaac gactgctggc ggaaacgggt ttcttttcat
     1681 agcggccttc gatccgcaca tacaagcctg gaacctggat acgggcattc cctgccgtat
     1741 cgataaagtt gatctggctg tcggcgcaat attccgccag tgcagacgtc aggcggttac
     1801 acaccagcag cacaggaaaa tccgcaggaa agcgaaccat ttgttcgcga acagccatca
     1861 gtgactcttt acggtggata aattttactt ccagagcaaa tatcactgac tcaccatccg
     1921 gccctgttag cttaccccag gcatcgtgct taccatgtct accgctatgt tcatactgca
     1981 aatggaatcc ttttggcaga ttctctatcg catcagacaa aagctgttct tccttcattt
     2041 gttcacacct cataactgtt catgaatcat gaacaaaaca atgttatgaa caaataaaga
     2101 aagaagcaac gtaaatgatc aaaaaaagac cgccagaaat gcgatgcctt attcaacctt
     2161 ccaaacgtca ttttccatta aagaaagaag ggtaaagagt agttggacgc attctttcag
     2221 ggttgagtaa ccattcctac tcccccgact ccacctcctc ctgcgccgcc atcagaacca
     2281 actctgactt cgaaatcagc aggtctttcg gcctcccacc tctcaacctg cgattagccg
     2341 agccaaacca gatagccagc ccccacggtg ttttacgctc cttaaacagt gaaagaatca
     2401 aacggacaac cttgagaggc cgcattttct catccagaac ataatcaggg tataaatcct
     2461 caccatcgac cttcagcgag aatatcttcc cggcggcttt ccagcctttc ggaccagcat
     2521 caggatcggc ggaacgaaag cccgcttttg cggataattc agacgccgtc agccattccg
     2581 agtcagccag tattctggct ttcagctcct ctagctgccg gtcattgcgc tcctggaaac
     2641 tcatatggtc gagtgcgcct cccggcgact ttttgaagcc atacggcaca acatgcctga
     2701 accggtttgc atccagataa tccgcccacc actgcatcat ctcccaacgt tcttcgagat
     2761 gctgcgcctt gtggatatac gccgcacgca cgcgcttgcg ctcctgatgg ctcatctggc
     2821 gctctaccgc gtcgcttgac cacagccccg actccaccag cgcgctacag gccatagtgc
     2881 gaaaaccgtg gccacagaca tctttctggg tgtcgtagcc catcacccgc agcgctttgt
     2941 tgatggtgtt ttcactcatc ggcttgcggt aattgtgatc gccggggaac accagttcag
     3001 ttcccggtcg gcaatgctgc cgcacctcgt gcagcagttc gatggcctga cgtgacaatg
     3061 ggaccaggtg tggggagtgc atcttcgccc cacgcgctga atatttaacg ccggggatcg
     3121 gctcacgctc agcggggatg gtccacatgg cgttacgcag atcaatttca tcccagcggg
     3181 caaagcgcag ttcgctggag cggataaaca gcagcagtgc cagttttacc gccagttgag
     3241 tcagttgacg gcctttgtaa tcgtcgattc tttccaggaa atcagggata agattcagat
     3301 ccaacgccgg gtgatgccgt actttgggcg tggagaccgc gccggttaaa tctgatgcag
     3361 ggttatgatc gataatgccg ttctggacgg cgtagcgcat cacgcaggca gtgcgctgct
     3421 gaagccggga agcaacatcc agtttgcccg ctttctccac ttccttgatg ggcaccagca
     3481 gatctttgac cttcattttg gtgatgtcac agctgccgtt cgtggggaat acgtaaagct
     3541 cgaaataacg cagcacccgc ttcttatgtt ccgccgacca gttcacgtta ctggtgaccc
     3601 attcacgtgc cacagactca aacgtatgtt ccggcgttat cgcaaggcgt tctgcttttc
     3661 ggcgtgcact cggatcgatg ccggaaatca gcaataattg tgcctcttca cgcatgcgtc
     3721 ttgcctgtgc aagcgtcaaa agcggataag ggcctaaagc gagctttttc tctttgttta
     3781 caaagcggta cttgatgtac cagcgttttg aaccgttagg gttaaccagc aggtaaagtc
     3841 catgcgagtc actgagctta tacggcttgc cacaaggctt ggcgtggact atagcgcgat
     3901 ctgttagtgc catttggggg cctcactatt atcgaactac gtctaagccc ccaatttggc
     3961 ccccaaatcg cgtggatgtc aatggatgaa aaatgaccaa cacggaaaga ccacaaaata
     4021 aatatggtta ttgtggaaaa ataaattacc tttgaattac aaacggatat ggacgaattt
     4081 ggacggaaat ggacacaaaa aaagccaccc ttaggtagct aattattgca tcacttggtg
     4141 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     4201 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     4261 cgcacgccac catgcaagcg ccagcttgct cgcacctcgc taagtgctga aattttcagc
     4321 attgcatacc gcgatgtgta acctttgtca cactccaggc gccccgccct gccatgctct
     4381 acacttccca gaccacacca gagaaggata aaaacatgtc gatgataaaa agttatgccg
     4441 caaaagaggc tggcggcgaa ctggaagttt atgagtacga tcccggtgaa ctgaagccac
     4501 aagatgttga agttcaggtg gattactgcg ggatctgcca ttccgatctg tcgatgatcg
     4561 acaacgaatg gggattttca cagtatccgc tggttgccgg gcatgaggtg attgggcgcg
     4621 tggttgcatt gggtagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     4681 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     4741 actgcgagca aggtgcggtg ccgacgattt tgaatcgcgg cggttttgcc gagaagttgc
     4801 gtgcggactg gcaatgggta atcccactgc cagaaaatat cgacattgaa tctgctgggc
     4861 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcca
     4921 ccagccgcgt cggcgtgatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     4981 acgcaatggg atgcgaggtg acagccttta gttctaatcc ggcgaaagag caggaagtac
     5041 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactc aaagcactgg
     5101 cggggcagtt tgatctcatt atcaacaccg tcaacgtcag cctcgactgg cagccttatt
     5161 ttgaggcgct gacgtatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     5221 ctgttccggc ctttacgtta attgcgggcg accgcagcgt ctctggttct gctaccggca
     5281 cgccttatga gctgcgtaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacta
     5341 ccgaactgtt cccgatgtcg aaaattaacg acgctatcca gcatgtacgc gatggtaaag
     5401 cgcgctaccg cgtggttctg aaagccgact tctgatccct ttgccgggtg cctgtcgccc
     5461 ggcgtttaaa tctctttgga aggcgtctcg caatctgctg aatttagcgg tgaagagtgc
     5521 gcagccgttg cctatactcc aggcaggaaa ctggaggata tctcatgagc gaacccctgt
     5581 taattgcccg cacgtcggac acagaactgt ttttattgcc gggaatggcg aatcgtcacg
     5641 ggctgattac tggcgcaacg gggacgggta aaaccgttac gctgcaaaaa ctggcagagt
     5701 ctctatctga aatcggcgtg ccggtgttta tggccgatgt gaaaggtgat ttaacgggag
     5761 tagcggagga aggtacttct tcggaaaaac tgctcgcaag gcttaaaaat atcggcgtaa
     5821 atgactggca acctcatgct aatccggtgg tggtgtggga tatctttggc gagaaaggcc
     5881 acccggtgcg ggcgacggtt tcggatctgg ggccgctgtt gctggcgcga ctgttgaatc
     5941 tcaacgatgt gcaatctggc gtgctgaata ttatcttccg cattgcggac gatcagggat
     6001 tgttgctgct cgactttaaa gatttgcggg cgattaccca gtacatcggc gataacgcca
     6061 aatccttcca gaatcagtac ggaaatatca gtagcgcatc ggttggtgcc atccagcgcg
     6121 gattactgtc gctggaacag caaggcgcag cacacttctt tggtgagccg atgctggata
     6181 tcaaagactg gatgcgcacc gatgccaacg gtaaaggcgt tatcaatatc ctcagcgccg
     6241 agaaacttta tcagatgccg aaactgtacg ccgccagcct gccgtggatg ctttcagagt
     6301 tatatgaaca attgcctgaa gcgggcgatc tggagaaacc aaaactggtg tttttcttcg
     6361 acgaagcaca tctgctgttt aacgatgcgc ctcaggtact gctggataag attgagcagg
     6421 tgataaggct tattcgctca aaaggcgtgg gcgtctggtt cgtttcgcaa aacccgtctg
     6481 atattccgga taatgtgctc gggcagctcg gtaatcgtgt tcaacacgct ttgcgggctt
     6541 ttacgcccaa agatcagaaa gcagtaaaag ctgcggcgca aaccatgcgg gccaatccgg
     6601 catttgatac cgaaaaggca atccaggaac tggggaccgg cgaggcgtta atctcgtttc
     6661 tcgatgcaaa aggaagtcct tctgtggtgg aacgggcgat ggtgatcgca ccttgttcgc
     6721 gaatggggcc ggtgacggaa gatgagcgta atggcctgat taatcactcc ccggtgtatg
     6781 gcaaatatga agatgaggtg gaccgcgagt ccgcctatga gatgctgcaa aaaggctttc
     6841 aggccagtac cgagcagcaa aataatcccc ccgtgaaagg gaaagaggtg gcggtggatg
     6901 acggtattct tggtggattg aaggatattt tgtttggcac taccggacca cgcggcggga
     6961 agaaagatgg tgtggtgcaa acaatggcga aaagcgccgc tcgccaggtg acgaatcaga
     7021 ttgtacgcgg gatgttgggg agtttgctgg gggggagaaa aagataagtc agacgtgatc
     7081 tgtgcccctc accctaaccc tctccccaaa ggggcgaggg gaccgttcga actcgtattt
     7141 gactttgtca acaataataa agggagcttt cgctcccttg ttagattacg attttctcat
     7201 taacagccac aggctgatta agaagaagct ggcgcttggc aacagtgcgc cgatgatcgg
     7261 cgggatgcca taaaccaacg tcagcgggcc gaagatctgg tccagtacgt agaagacaaa
     7321 accgaagctg ataccggtga ccacacgcac gcccatcggt acgctacgca gtgggccaaa
     7381 gatgaacgac agcgccatca gcatcatcac cgccacggat agcggctgga agattttgct
     7441 ccacatgttg agctgataac gtccggcatc ctgaccgctc gacttcagat acttcacgta
     7501 gttgtgcaaa ccactgatgg agagtgcatc cgggtccagc gccaccacgc ccagtttgtc
     7561 tggcgtgagg ttggttttcc aggtgccgct caccgtctgc gaaccggtaa tctgtttcgg
     7621 attggtcagg tcagattcat caacctgcga cagacgccag actttatgtt ccgggtcaaa
     7681 cttcgcggta gcggcataac gtacggattg cagacgacga ttctcgttaa aggcataaat
     7741 actgatgcca cctaactcct cgtcaccttt aacccgctca atgtagacga agttgttgcc
     7801 atctttcgcc cataagcctt gctgggtaga gagcaacgag ccgccgtaca tcgcctgcgc
     7861 acggtagtta cgcgccatct gctcgccctg cggcgcgacc cattcgccaa tcgccatcgt
     7921 cagcaagacc agtggaatgg cggttttcat caccgacagc gctacctgca tacgggtaaa
     7981 accagaagcc tgcatcacca ccagttcgct gcgctgcgcc agcatcccaa gaccaagcaa
     8041 cgccccaagc agagccgcca tcgggaagaa gatttgcaca tctttcggca cgctcagcaa
     8101 ggtatacatt cctgcgccta acgcgtcgta actcccctgc ccggcttttt tcagctgatc
     8161 gacaaacttg ataatgcccg acagcgacac cagcatgaac agcgtcatca tgatggtggt
     8221 gaaaatagtt ttaccgatat agcggtcaag tacgccaaaa ggttgcatca caccgctcct
     8281 ttacgcgaaa aactggcgcg caggcggcgg accggcacgg tgtcccaaag gttgagaaca
     8341 atcgccaaag ccagataaat caggttaacg gtccacatcc acagcgtcgg gtccagctta
     8401 cctttaccgc cgttcgattt cagggaggtc tggatcagga agaaaagtag atacagcagc
     8461 atggctggca gcatcgacag tacgcgtccc tgacgcgggt taaccacgct cagcggtacg
     8521 accataagtg ccatcataaa cacggtgaat accaacgtga tacgccagtt cagttctgca
     8581 cgggcacgat cggtgtcagt gttccacaat gtgcgcatgt ccatctggtc ggtatcgttc
     8641 gggtcgagcg ccaccgcctg gtgaccaatg atcgcctgat aatcctggaa gtccgtaatg
     8701 cggaaatcac gtaacagtgc agtgccttcg aagcgcgttc cctggttgag agtgacgacc
     8761 tgggagccgt cgcgcagctg ggttaaatgt ccggaatcgg ccaccaccac agaaggacgt
     8821 gcattacctt ttggtcgaat ttgcgcgagg aacacatctt tgaaatcgct gccgtcaacg
     8881 ctttcgatga acagcaccga gctgccatta gtcgcttgct ggaattgccc ttgcgccagc
     8941 gccgccatgc cagggttcgc tttcgcttct gctaacactt catcctgatg acgcgatgac
     9001 cacggtcccg cccacatcac gttaaccgcc gcgacgattg ccgtgaatac cgcaaggatc
     9061 attgccgctt tcaccagaac cgctttgctc aggccgcagg catgcattac cgtaatttca
     9121 ctttcggtat acagtttgcc cagcgtcatc agcagcccga ggaacaggct taatggcagg
     9181 ataagctgcg ccatttccgg cacgcccaac ccgagaaggg agagcaccag attcgccgga
     9241 atatcgccgt caaccgctgc gccgaggatc ctcactaact tttgacagaa gaagatcaaa
     9301 agcaagatga agagtatcgc cagctggctt ttgagcgtct cccgcaccag atatcttatg
     9361 attatcactt taaatacgcc cgtaaaaact cgtcttttgc aggattttag cttgtttcat
     9421 ggcttaaacg tcatttattc tcttgagtcg tcgaaatcgt cgctaagata attatactca
     9481 acggattcac ctctcagatt ttgttctgac gtgccaatgc cgtaataacg ttaagattaa
     9541 cacgaagtca tcgcaacagc ggacatgagt tacgaaagct tgcaattcta tctgtagcca
     9601 ccgccgttgt ctttaagatt caggagcgta gtgcatggag tttagtgtaa aaagcggtag
     9661 cccggagaaa cagcggagtg cctgcatcgt cgtgggcgtc ttcgaaccac gtcgcctttc
     9721 tccgattgca gaacagctcg ataaaatcag cgatgggtac atcagcgccc tgctacgtcg
     9781 gggcgaactg gaaggaaaac cggggcagac actgttgctg caccatgttc cgaatgtact
     9841 ttccgagcga attctcctta ttggttgcgg caaagaacgt gagctggatg agcgtcagta
     9901 caagcaggtt attcagaaaa ccattaatac gctgaatgat actggctcga tggaagcggt
     9961 ctgctttctg actgaactgc acgttaaagg ccgtaacaac tactggaaag tgcgtcaggc
    10021 tgtcgagaca gcaaaagaga cgctctacag tttcgatcag ttgaaaacga ataagagcga
    10081 accgcgtcgc ccgctgcgta agatggtgtt caacgtgccg acccgccgtg aactgaccag
    10141 cggtgagcgc gcgatccagc acggtctggc gattgccgcc gggataaaag cagcgaaaga
    10201 tctcggtaat atgccgccga atatctgtaa cgccgcttat ctcgcctctc aggcgcgcca
    10261 gctggctgac agctacagca agaatgtcat cacccgcgtt atcggcgaac agcagatgaa
    10321 agagctggga atgcactctt atctggcggt cggtcagggt tcacaaaacg aatcgctgat
    10381 gtcggtgatt gagtacagag gcaacgcgtc ggaagatgca cgcccaatcg tgctggtggg
    10441 taaaggttta accttcgact ccggcggtat ctccatcaag ccttcagaag gcatggatga
    10501 gatgaagtac gatatgtgcg gcgcggcggc ggtttacggc gtgatgcgta tggtcgcgga
    10561 gctgcaactg ccgattaacg ttattggcgt gctggcaggc tgtgaaaaca tgcctggcgg
    10621 gcgtgcctat cgtccgggcg atgtgttaac caccatgtcc ggtcaaaccg ttgaagtgct
    10681 gaacaccgat gccgaaggcc gcctggtact gtgcgacgtg ttaacttacg ttgaacgttt
    10741 tgagccggaa gcagtgattg acgtggcgac gctgaccggt gcctgcgtga tcgcgctggg
    10801 tcatcatatt actggtctga tggcgaacca taatccgctg gcccatgaac tgattgccgc
    10861 gtctgaacaa tccggtgacc gcgcatggcg cttaccgctg ggtgacgagt atcaggaaca
    10921 actggagtcc aattttgccg atatggcgaa cattggcggt cgtcctggtg gggcgattac
    10981 cgcaggttgc ttcctgtcac gctttacccg taagtataac tgggctcacc tggatattgc
    11041 aggaaccgcc tggcgttctg gtaaagcaaa aggcgcaacc ggtcgtccgg tagcgttgct
    11101 ggcacagttc ctgttaaacc gcgctgggtt taacggcgaa gagtaagttt gcgttttacc
    11161 ctcaccctaa ccctctccct aagggagagg gaaccgatcg agcgcaatag aaattcattg
    11221 ctcatctttc agggtgagga atgcagcaat atttaaatcc accacaagaa gccccatata
    11281 tgaaaaacgc aacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg
    11341 ttgagcaact ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag cgcgtactca
    11401 tcgcctgtga agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg
    11461 cagaaagctt tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg
    11521 agatcgcctg gccgcaaaag cgtagcagca gcccgcgcga tatattgatt agtctgcgaa
    11581 caagctttgc agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag
    11641 attctctgaa acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc
    11701 tgaatacggc aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc
    11761 gctttacgag cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga
    11821 aagtttctgc atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc
    11881 cttccagcaa accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac
    11941 cctgtggcag gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa
    12001 gattgccgca gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa
    12061 aatctgggaa tggaaagcgg aatctggcgg caccattacc cgtcagatgc gccgtctcgg
    12121 caactccgta gactgggagc gtgaacgctt cactatggac gaaggcctgt ccaatgcggt
    12181 gaaagaagtt ttcgttcgtc tgtataaaga agacctgatt taccgtggca aacgcctggt
    12241 aaactgggac ccgaaactgc gtaccgctat ctctgacctg gaagtggaaa accgcgaatc
    12301 gaaaggttcg atgtggcaca tccgctatcc gctggctgac ggcgcgaaaa ccgcagatgg
    12361 taaagattat ctggtggttg cgactacccg tccggaaacc ctgctgggcg ataccggcgt
    12421 ggccgttaac ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc
    12481 gctggttaac cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac
    12541 cggctgcgtg aagatcaccc cggcgcacga ctttaacgac tatgaagtgg gtaaacgtca
    12601 cgccctgccg atgatcaaca tcctgacctt tgacggtgat atccgtgaaa gcacccaggt
    12661 gttcgatacc aaaggtaacg aatctgacgt ttattccagc gaaatcccgg cagagttcca
    12721 gaaactcgag cgttttgccg cacgtaaagc ggtcgttgct gcggttgacg cactcggcct
    12781 gctggaagaa attaaaccgc acgacctgac cgttccttac ggcgaccgtg gcggcgtagt
    12841 tatcgaaccg atgctgaccg accagtggta cgtgcgtgcc gatgtcctgg cgaaaccggc
    12901 ggttgaagcg gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta
    12961 cttctcctgg atgcgcgata ttcaggactg gtgtatctct cgtcagctgt ggtggggtca
    13021 ccgtatcccg gcatggtacg acgaagcggg taacgtttat gttggccgca acgaagacga
    13081 agtgcgtaaa gaaaataacc tgagcgcgga cgtcgcgctg cgtcaggacg aagacgttct
    13141 cgacacctgg ttctcctccg cgctgtggac cttctccacg ctcggctggc cggaaaacac
    13201 cgacgcactg cgccagttcc acccaaccag cgtgatggta tccggcttcg acatcatctt
    13261 cttctggatt gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa
    13321 accgcaggtg ccgttccaca ccgtttacat gaccggcctg attcgtgatg acgaaggcca
    13381 gaagatgtcc aaatccaagg gtaacgtgat tgacccgctg gatatggttg acggtatttc
    13441 gctaccggaa ctgctggaaa aacgtaccgg caatatgatg cagccgcagc tggcggacaa
    13501 aatccgtaag cgcaccgaga agcagttccc gaacggcatt gagccgcacg gtactgacgc
    13561 gctgcgcttc accctggcgg cgctggcttc taccggtcgt gacatcaact gggatatgaa
    13621 gcgtctggaa ggctaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct
    13681 gatgaacaca gaaggtcagg attgcggctt caacggcggc gaaatgacgc tatcgctggc
    13741 ggaccgctgg attctggcgg agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga
    13801 cagcttccgc ttcgatatcg ccgcaggcat tttgtatgag ttcacctgga accagttctg
    13861 tgactggtat ctggagctga ccaagccggt aatgaacggt ggaacagaag ccgaactgcg
    13921 cggtactcgc catacgctgg tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat
    13981 cattccgttc atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc
    14041 cgacaccatc atgctgcagc cattcccgca gtatgatgca tctcaggttg atgaagccgc
    14101 actggccgac accgagtggc tgaaacaggc gatcgttgct gtacgtaaca tccgtgcaga
    14161 aatgaacatc gcgccgggca aaccgctgga gctgttgctt cgtggttgca gcgcggatgc
    14221 agagcgtcgc gtaaatgaaa accgtggctt cctgcaaacg ctggcgcgtc tggaaagcat
    14281 caccgtgctg cctgccgatg acaaaggtcc ggtttccgtt accaagatca tcgacggcgc
    14341 agagctgctg atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgcctggc
    14401 gaaagaagtg gcgaagatcg aaggtgaaat cagccgcatc gagaacaagc tggcaaatga
    14461 aggctttgtc gcccgcgcac cggaagcggt tatcgcgaaa gagcgtgaga agctggaagg
    14521 ttatgcagaa gcgaaagcca agctgattga acagcaggct gttatcgccg cactgtaatc
    14581 aacacagtga agaaaacgaa ggccggagca tgctccggcc ttttttatct cttacagaaa
    14641 tggtaaagaa ggcataactc cccgagaaat actggctaag aagcctttat ctggttgttc
    14701 ttctttattg attaaaggaa ggtcatccaa atcgcccagc aaatacgtat tttttgcctg
    14761 ccagtcaatc atccatattt tcagcagtgg ataagccaga ccgcccgtaa tcccggatat
    14821 caccaccaac gcacacatgc gataaagcat accgtggtac gttaaagttg agcgaaaacg
    14881 aatacgccca tcattcagtg acaggttgct cataaaatga tttcgcaacg acaccgttaa
    14941 ataactggtg ctaacagcaa tcccaaaata atagattaac tgcgcgatta tcattttacg
    15001 ttgcatttcc ataaattgct gtaaattctc aatgtcatca tttgcatata cagaactatc
    15061 a
//
LOCUS       DAZGAP010000136        15003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGAP010000136 DAZGAP010000000
VERSION     DAZGAP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.137"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..401)
                     /locus_tag="ACHLAM_003656"
     CDS             complement(<1..401)
                     /locus_tag="ACHLAM_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7855120.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDE"
     gene            1742..3232
                     /locus_tag="ACHLAM_003657"
     CDS             1742..3232
                     /locus_tag="ACHLAM_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO7855121.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGESLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(3354..3926)
                     /locus_tag="ACHLAM_003658"
     CDS             complement(3354..3926)
                     /locus_tag="ACHLAM_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855122.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(4012..4218)
                     /locus_tag="ACHLAM_003659"
     CDS             complement(4012..4218)
                     /locus_tag="ACHLAM_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7855123.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            5075..5299
                     /gene="hha"
                     /locus_tag="ACHLAM_003660"
     CDS             5075..5299
                     /gene="hha"
                     /locus_tag="ACHLAM_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO7855124.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(5631..5828)
                     /locus_tag="ACHLAM_003661"
     CDS             complement(5631..5828)
                     /locus_tag="ACHLAM_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855125.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            6027..6596
                     /locus_tag="ACHLAM_003662"
     CDS             6027..6596
                     /locus_tag="ACHLAM_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7855126.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            6857..7258
                     /locus_tag="ACHLAM_003663"
     CDS             6857..7258
                     /locus_tag="ACHLAM_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7855127.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            7246..7680
                     /locus_tag="ACHLAM_003664"
     CDS             7246..7680
                     /locus_tag="ACHLAM_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO7855128.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            8455..8681
                     /locus_tag="ACHLAM_003665"
                     /pseudo
     CDS             8455..8681
                     /locus_tag="ACHLAM_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(8683..9465)
                     /locus_tag="ACHLAM_003666"
     CDS             complement(8683..9465)
                     /locus_tag="ACHLAM_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO7855129.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            9768..10688
                     /gene="rbsK"
                     /locus_tag="ACHLAM_003667"
     CDS             9768..10688
                     /gene="rbsK"
                     /locus_tag="ACHLAM_003667"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO7855130.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            10716..12032
                     /gene="fucP"
                     /locus_tag="ACHLAM_003668"
     CDS             10716..12032
                     /gene="fucP"
                     /locus_tag="ACHLAM_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO7855131.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            12044..13057
                     /locus_tag="ACHLAM_003669"
     CDS             12044..13057
                     /locus_tag="ACHLAM_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO7855132.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            13479..13763
                     /locus_tag="ACHLAM_003670"
     CDS             13479..13763
                     /locus_tag="ACHLAM_003670"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7855133.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(13979..>15003)
                     /locus_tag="ACHLAM_003671"
     CDS             complement(13979..>15003)
                     /locus_tag="ACHLAM_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7855134.1"
                     /translation="VPNNEAIVSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFL
                     TGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDV
                     AIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTC
                     LFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLV
                     PNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGA
                     AAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLGDIGFANTTFSDADFGALGIL
                     LGIIVR"
BASE COUNT         3941 a         3351 c         3727 g         3984 t
ORIGIN      
        1 tactcatcca cagacagggc ccggtcatcg gctttaatca gccacagcac caggtcaagt
       61 tcaggcagaa tgtcacggta cagggcctca tactctgcat ccctgtcccg gctctcgccc
      121 accccgggca ggtcagtgat aaccatgctg tgaccgtggc cactcagacg gaagcgccgt
      181 acttcccggg tgccggcgtg aacatcactg accggggtga cctccccctg aaacagtgca
      241 ttacagagtg aggatttacc ggccccgctt ttacccataa tgccaatcac gggttcgtga
      301 ctggtgagtt tacgcagatg ttccaggatg tgacgggaaa gtgagtaagg cagggaggaa
      361 agcggttttt caattgcctc aatggcatca gacggattca tacatgtatt cccggtgaaa
      421 aaagacaaaa ccccgcgcac cggaaaggtg gcgggggatc cacggagata atatctgtct
      481 gaaattgttt tgaatcgatg ggattgttgc gtattaacat gaaggagatc aggacttatc
      541 cgttactggg tgatctcctt ccatgaaaat acgcggttat tgttaaacag caggctgatt
      601 actccgtaaa ggagaaaaat attcatcttg cagcctgatt atcaggtgtg tttatgttta
      661 ctgaggtgag tatttttcag tatattttcg gggcagtcag tgggagatat taaatttacg
      721 tttagagtta tcttgcttct gctgtgtatt tttataagag tgcgcacgta atgatatctg
      781 ttgataccat gttgttaagt gtttcccttc cgggtcatgc gtacttgcaa aatggcatct
      841 gaatcgatca cggtttggcc aaaccgtgat cgaatagaga ggaatacgca cgtaatgatt
      901 aaatgctttt gttacaccat tcttccggca tgaatattta tccagttccc ttttttattt
      961 gtgtagtgat actgtcggaa tagcgttcag taatgtgaag tgtcctgtta aaaatcttta
     1021 ataaatgtgt caccctggtc aggaaatatc tgctgagata ttattagttg gtggtgtttt
     1081 tactgagtaa tagctggctt ttaattcctg aactgaaatg ctgtcgctcg tcagggttta
     1141 attaaaactg gatgatgatg tgaaaatgaa ggctggtttt tgtgtttgtc gaggaagtgt
     1201 tgtttttatc cattctcatc attggctggc tgcgattata cagaagtatt tgaagaaaaa
     1261 atggtgcatt ttatctgtgc cggcagggag gaggattttc ctgatatgtc agtattaaat
     1321 ggtttttgtt atttataaaa atgtcatcct gtccatagaa tattgtactg agtactcagc
     1381 attcagcgca ggaactgtca tcttctttat tttattcttc ttcaggaggg cagttttctg
     1441 aaagcgttac tccggggaaa ctgtttatgt ttattcccgg caggaaaccc gtacctgcaa
     1501 taccctggat gatgtttttt gctggtacgt tgttgtgtga aaattaatca acatgtgtgc
     1561 tccggagagc gcccgtagac gcacagattc attctgacat tctttagcga caaaataccg
     1621 ggtggttatt atttaacctg aaaatcgaca gaaattttta tcctgtattg attctgctgc
     1681 tatttttgct attagtagca atagttgcgg tactttatgt tctgtgtgtt gtgaggtcat
     1741 tatgccatac tttcagtatc ctgatgagtt tcccctgagc tcgctgcctc cactcattcg
     1801 tgatgccgtt attgaagctc agcaaatcac gcaggctccc ctggggcttg tggcagcatc
     1861 tgccctgggc gctgtttcac ttgtctgcca gaatctgatt gatgtctgca gactgaatac
     1921 actgcggggg ccggtttctc ttttcttact gacgcttgct gagtccggag aacgaaaaac
     1981 cgcagtggac aaattattaa tggagcccct gtaccagcag gagatgctgt tatattccag
     2041 acacaaaaat gaactgacga catggaaaaa taaggaagag cttttaaagg cgcagaaaaa
     2101 agcattgttg tcaaaactga ataaagaact ccgcaaaggc gcggatgagt cagaaacgct
     2161 cagacaactg gaagcgcttc agaaaaacag aggggaaaaa cctgtaagat ataaattcat
     2221 ttttaatgat gccacgacag cagcaattaa agatcagctt tgtggtcagt ggcgttcagt
     2281 gggtatcatg tctgatgaag ccggtataat atttgatggg tataccctgt ctgagctgcc
     2341 ttttattaac aaaatgtggg atggctccgt attgtcggtg gacagaaaga atgaacccga
     2401 acagatgatt gaaaatgcca gaatgacatt atccctcatg gtacaacccg gtctttttga
     2461 ccgatatatg gagcgaaaag gttctgttgc cagggacagt ggttttcttg cccgttgtct
     2521 aatcagtaaa cctgccacca cgcaggggaa acgctttatt aacggagcag tgaccccggg
     2581 agaatcatta acggcatttc atgaacgatt aatggaactg gccagaggca gtattgaaaa
     2641 atccagtaag gatgaacgtt attgtctgca tttttctcca gaagcgcaaa aaatatttat
     2701 tgatcactat aatgttctgg agcaggattt gtctccttcc ggacccttaa gccagtttcg
     2761 ggggcatgtt tcaaaaaaaa cagaaaacat tgcgagaatt gctgcgttat tgcagtattt
     2821 cagtcagggt gaagggaaaa tctctgccga tatcatgacg tctgctgtcg taataagttc
     2881 gtggtacaca gatgaatata aaaaattatt tgcgttacct gatgaatctg aactgcagct
     2941 gcaggacgcc cgggagctgc tcgactggct cattgaggag tgccgcggag aatgcccgcc
     3001 ccgtgtcagg aaaaattata ttctccagtg tggcccgggc cggttcagga acaggaaaaa
     3061 gctgaacgtc ctgctgaaca ttcttgcatc tcaactcagg ctctctgtcg tgcaggaagg
     3121 aaaaaccacc tatgtcctgc tttccgaaat aaacaatatc acgctgcctg aactgaatgc
     3181 gtgctacagc cagaaactga taagatggca tgagcaatcc tttggcttat aatgttccct
     3241 gttgctgaga cggattattc agtggtggta tcgcagtatg cccgtcaccc cccgcttctg
     3301 catgagggtg acggaggaaa agtaagtgtg cggaggcccg ggagcgttat gaatcaacag
     3361 gcgtataaca ggtactcgtc aatcatcagt tccagctcgc gcttttcccg tttgcgcgcc
     3421 acagcccagt tttcaagctc tgcatgtgat ttatgcttta actcttcagt ccagggctgc
     3481 atggagtggg cacacatcag atcataccag cacagccata tcagcgtgtg gtagagatca
     3541 cgatcgtcga aacgccggag caggctgttc agcctgtttg ccagccggtt ttcacccaga
     3601 cgatgtagct cactgagtaa aacgccgccc agattaaaat gtgtaagcag catgatttca
     3661 tgggtctgaa tgctggtcat taacccttcc tgaatggcag gaatgaacgg tggtgttccc
     3721 gggttgacga cctgatagag tccctgtggt gccactgaga tgatagtgcc tttttccggc
     3781 agaaaaaagt gggccagggt caggcgatga taaccatgaa gatgcggaga ccccattcca
     3841 tgagcgacga taagcctcgt cacgcttgtc atccatttct gatgctggat gagcagcagc
     3901 tcacaccggt tttcaagttt ttgcataata ttctctcctt tagcgcgcag caaaatgcct
     3961 ccgggggccg gatcatgcct ttgctgcggt tgaacggata acggtcagga attacggacg
     4021 ggactgtgca atacgcgcct gcagccaggt ttccacttca cttttcagcc atcgggagct
     4081 acggcccagt ttgatggggg caggaaaggc accatccttg atgagcttgt aaaaccattt
     4141 atcggtcagg ccggtcagtt gagtgataaa tgccatgtcg accatctggt catccatcag
     4201 tgaaactggg gtattcatga gaatgttcct tcggatgttg ttaatctcaa ccggcgttgg
     4261 tgatgaaaac aacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaataa
     4321 tcgggaatgt gaccgtcagg agataaacgg ggggattgcg gtggctgagc cgtaccggta
     4381 tgtcgggtga ttgttgcgaa acgatgcttc cgcacgcgca cgggaggact ttcgtgagac
     4441 aaggggaaaa ctggcgttct gataattgca gagttgtttt tgaaggcggt tgtggtgcat
     4501 cagcctgtga cctgtgggca ggcgacaggt atcactggtg ggataagact gtctgattgg
     4561 tattataaat atataagaga gctcaaacac atgagttcat atatgtgaaa taaatctgtg
     4621 agtgtgatca gcagaccagt ctacccagta gcaacagatt gcgttgtact cacagggtta
     4681 tccctgataa ctggcgtata acctgcgtac ataaaacgta cctgcaatgc acctgaacag
     4741 agtgcattaa gcatccgcct gagcggtaaa cgtatccgat ctggtgagtg acagtctgag
     4801 agcattctca gtaatcgccc ccttgtatcc tggaaattgg ttaagagcga ttagatttgg
     4861 caaggtgtat gcatccagca gcttaacacc tcagctaact ggctggttac cttcaggttg
     4921 tcgcaatggc atcggacatc cggtgtcgga taactaaacg tagcgttaag gcgatattgc
     4981 tgagagaccg gttaagtggt ggcacgggag taatgagttt ggggagggta ccagaatgcc
     5041 agtcaggtgc ggtatgctgg cagtatcacg ttgattgcag cataaagtaa cgagagacga
     5101 atggaagtta aaaccagagc agttccgtcg ttgttcatcc cgggagacgc tggaaaaagt
     5161 gattacccgc acacgttata aacttacccc tgcggagctg gaagccttta actctgcggt
     5221 cgatcaccgg ctggcagaac tgacaatgaa caaactttac gatcgcgtgc cggcttccgt
     5281 ctggaaatat gtcacctgaa tcttgaatcc gcgctacgat gactctggtg ttatctgtgc
     5341 accggaagat cgggaacatt aagcagtata acggcaacaa agaattcgaa atcaaaaggg
     5401 aatggtgtac agaggggcag gcgttcaggc attaccggcc tgtatcttct gaaggggact
     5461 cgtatttgtc ggggctgagt attccgctcg attcgatcac ggattggcag aaacctgact
     5521 gtgattgatt caggtaccat tttgcaggga tccggtgcgt tactgtctgg cacaacaaac
     5581 gcagcgggtc gctctgcccc gccgattcct gataattcct cccctgtaac ctactgcaac
     5641 agtacaaata cactgacgcc tgaaacggta accagaatca gcaccagcag taacgtcagt
     5701 aaacagagca gacgatcggt cttccggtaa taaccgaagg ggtcgacagc gccacaatac
     5761 gggcaacaca ctgtattatg agtaattgcg tttccacagt ccctgcactt ctttttgcgt
     5821 atcacgatcg ctccctctcc acgccttatc cgtaacctgt tttttacatt aaaaaaacaa
     5881 cgccgggaat tcaatcgtca gtccggagac ggccgttcgg gttatcacag agtgcctgag
     5941 acagtgtcct gccggaggtc acctcagggg ctctgcgcat tttttacgac gtggctattc
     6001 cgtatgaacc atacggagat ttaaccatga cctacaaata caaccccttc tggcagcaac
     6061 gtattcgtga gacggtgcgg cacgcactgg atgttcatcc ccgcctgacg gcacttcggg
     6121 ttgatctgcg tctcccggat gtgccggcag caacggacgc ggctgtgata tcccgcttca
     6181 tcaatgccct gaaagcccga atcgacgctt atcagaaacg taagcatcgg gaaggtaaac
     6241 gcgtgcattc ctcgaccctg cattacgcct gggcccggga gttcggggag cttaaaggca
     6301 aaaaacacta tcacctgctg ctgctggtca accgggatac ctggtgtcgt gccggcgatt
     6361 accgcgctcc ggggtcactg gccgggatga ttaaacaggc ctggtgcagt gccctgggag
     6421 tggatgccgg acgttatgac acgctggcac attttcctga caagccagcg gtgtggctgg
     6481 aacgcgatga tgaaaccggc tttcagcagg tgctggaacg ttctggctat ctggcgaagg
     6541 agcataccaa agctcgcggc accggtgagc gcaattttgg ctgtagccgg agttgaacac
     6601 gacaggcgca ccaatacccg gcaaaccaaa agtatgggtt gttgtctggc ggcaacgatg
     6661 tatccactcg cccactgact gttttttacg tcagtgggcc attgtgtgac aaccacagtg
     6721 ctgtaacagc aaccagcctg ctttacccaa actccagtcc agattttaac tgaaccgcca
     6781 ttcacaccct gaccacgctg cccgtaccgg gacggctgtt gcctgcgtgt cgtctggcgg
     6841 ctgaggaaca tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct
     6901 gacgggggcc cgtaccgcac agactgcgcc ttacgaccgc tacacctgcc acctttgcgg
     6961 cagtgcactc aggtaccacc cgcaatacga cactgaacgt ccctggtttg aacacactga
     7021 cgacgggctg actaagcacg gtcatgagtg cccttatgtc agaccggaac gcagggaggt
     7081 tcgcttgatt aagcgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc
     7141 cagctggcac tgcagacaat gtcaccacga ttattatggg gagcagtact gcacaaactg
     7201 ccagaccgga ggattcagta ttccccggac aactcaggag gaaatatgcg aattctgaac
     7261 tgttatatgg cgaatgacag caaaggccat tttgttacgg cgaaagaagc tgcgaagcac
     7321 aaccgacagg acgttttgtg ctgtgtgtcc tgtggatgcc cgttaacact tcagcggggc
     7381 aatgacggac aaccaccgtg gtttgaacat gaccagatga ctgtcgctga aaaaatcctg
     7441 ctgcgatgta cctggcttga cccggcagag aaagaggccc gtcgtttgca tctgcagggc
     7501 atgacggttc cggattatac ggtgaaggtg agaaagtggt tttgtgtgat gtgtgacgaa
     7561 gattatgagg gggaaaagtg ctgcccacgc tgcggtactg gggtatacag cagggcgtgg
     7621 gggcggcagg aggtgccgtc ggaagatgcc agggctgata atccgttaca gaggctgtaa
     7681 tggttgcctc cggagcagtt tgcggtaatg ccgttcccgg gaatggattg ccagtgcggg
     7741 gactgtggag cttaattccg gtgttggtgg cctttccgtt gattttgagc caacagccct
     7801 cctgtgactg acaggctgcc tcgtcattcc attcgtatct ccagtaacag ggggttgtat
     7861 tgtattttaa tgtgccggtt caggcgtgcg tttcccggcg cgtctgcacc ggcttaacca
     7921 aattcaacag ggataaaata gtgtaaataa ccgaagtatt acttgcagaa tcgggggaag
     7981 catccggggt tttattcaac aggcatggga ggctccggct ctgacagaca ttgtactgtg
     8041 gaatgttgtt acaggttggg gcggcaaccg tgccaggaga ccatgagccg ttcacggcat
     8101 taaaggccac acttcggtcg tttacattcc ctgtggtggc aggcatcttc cgttaaggcg
     8161 tcagagcgtt accaacgtta tgttgtcatg gctcaccttt catagtcgat gcttatctgg
     8221 tgactgaaac tagccgcaga taaagagtgt gaagttgata ccatggagtg gagtgacgtg
     8281 caggcaggag attcgctcta tgcagggttg tgagatgaat tcaaaccggc tgtcagcgcc
     8341 agtaatattt gaagattcgt ccggttgtta tccagtgtgc attaaaaacc cagacattat
     8401 ggacaataca acggcttcca taacagggag gagcctgttt attatccggg cgtaatgact
     8461 aacctttgtg ggctttttcc cgtcgtgctt ttaacgttga aggcattttc tgcctgccga
     8521 ttcaggacag cgacaaaagc catatcagac aattggtcaa tgatgaccag cgtctggagt
     8581 agatgataag ccaaattgat aaactggaat atgtgatgaa agtgcaacgt aatcagtcca
     8641 atccgacgat gtttaacaag agtacggtgt tttttcagta agtcattcgt cttctgaatc
     8701 aggtgtaatg atctttacag aagcattccg gcagtgcgta acataatctg ccggtaacgg
     8761 acgctcgcta atcagaacat caaatgcaga gagcggtgcg aggctggcag aggctacttc
     8821 atcgaacaag gcgtggcggg cgagcaaaat tttccttagt ccacgggcca tcgctttacg
     8881 cttagtggca agatcttcgg gattaaacca gctgacgcca aagtgatcat gtacaccgct
     8941 ggcggaaata aatatttttc gcggattgag agagtctaat ggcgaagagt tactggcatc
     9001 gtaaaaagca tcacttctgg cacgatacgt accaccacaa agtattgctg tcacattagg
     9061 tttttcattc aacgcaacaa agacacgatg tgagtaacag atgccagtga aggtgattgc
     9121 atccgggatc atgcttataa cgagcggtat ctcctggcca ttatcaaaga agatcagatc
     9181 attttcatta accattccgg cggccagaat tgcaataggt aagtcatcac gatgatttct
     9241 cggaacgtcc tggattactg gcatggatgg tgcgggttta tgcaccatta caatatagcc
     9301 acccagtagg gtcagtggca gaggttcatc ttcctgatgg agatcgcgac gaatagtcat
     9361 tacagaaact tccagcattc gtgccgcgtc tttcaaatgg attctgtcgg ttttcttaag
     9421 tatttcaatc aaacgtcgga tacgctcttt ttgcttcgtt tccatcaact ctctccgtaa
     9481 aatgttataa aaataacatt atggcattag taaacattgt tttagttacc atactatacg
     9541 accgaccctt tgaaatgaaa agggtataga ctgctgaatt ttcggtgtta atcctgttag
     9601 gttgcttatg attagatagt gttggcctga gtgataagac tggaagtcta ttaaataaac
     9661 atcgggttga tgtgctaatc gcgatctcag tcacaaatga tgcaaaagta acatgctaat
     9721 gttatttaaa taccattgtt atgcgaaagt ttataagagg tagtgaaatg gacattgcag
     9781 ttattggttc caatatggtg gatctcatta cttacactaa tcagatgcct aaagaaggag
     9841 aaactctgga agcacctgcc ttcaaaatcg gatgtggtgg gaaaggtgct aatcaagcgg
     9901 tggcggcggc taaattgaat tcaaaagtcc tgatgctgac taaagtaggt gatgatattt
     9961 ttgcggataa tactattcgt aatctcgaat catggggaat taataccact tatgttgaga
    10021 aagtcccctg taccagcagt ggtgttgcac cgatctttgt taatgccaac tcgagcaata
    10081 gtatattaat aattaaggga gcgaataaat ttctttcccc ggaagatatc gatcgagctg
    10141 cagaagatct gaaaaaatgc aaattgatcg ttttacaact ggaagttcaa ctggaaacag
    10201 tttaccacgc gattgagttt ggtaagaaaa atggtattga agtgttatta aatccggctc
    10261 cggcattgcg tgagttagat atgtcttatg cctgcaaatg tgatttcttt atcccgaatg
    10321 aaacagaact ggaaatatta accggtatgt cggttgatac ttatgatcat attcgtttgg
    10381 ccgcacgttc gttagttgat aaagggctga acaatattat tgttaccatg agcgaaaaag
    10441 gcgcattatg gatgacccgc gatcaggaag tccatgttcc tgcattcaaa gtaaatgctg
    10501 ttgatactag tggcgctggt gacgcattta ttggttgctt ttcccattat tatgttcaaa
    10561 gcggtgatgt tgaagccgct ttgaaaaaag ccgctctctt tgccgctttc agcgtcacag
    10621 ggaaaggtac gcaatcatct tatccgagta ttgaacaatt taatgaattt ttaaccttaa
    10681 acgaataatc atcctgcaca gttaaaggta gcactatgaa cgataaaaac atcattcaga
    10741 tgcctgatgg gtatctgaat aagactccct tgttccagtt tattttgtta tcgtgtttat
    10801 tcccgctatg gggatgcgca gctgcattaa atgacatatt aatcactcaa ttcaaaagtg
    10861 tattttcgct aagtaacttt gcttcagcat tggtgcagag cgcattttat ggaggttatt
    10921 ttttaattgc aataccagca tcccttgtga ttaaaaagac cagttataag gtggcaattt
    10981 taattggcct gacgctgtat attggtggtt gtacactttt tttccctgcc tcgcatatgg
    11041 caacctacac tatgttcctt gcagctattt ttgcgattgc gattggcctg agtttccttg
    11101 aaactgcagc gaacacatac agttcaatga tcggtccaaa agcctatgcc acattgcggc
    11161 tcaatatcag ccagaccttt tatccgattg gtgctgcgtc tggtatttta ttgggcaaat
    11221 atctggtttt ctccgagggg gaaagccttg aaaagcagat gtcaggaatg aacgccgggc
    11281 aaattcacaa ctttaaggtg ctgatgttag agaacaccct tgaaccgtat aagtatatga
    11341 ttatgattct ggttgtggtc atggtgttgt tccttttaac ccgcttccca acctgtaaag
    11401 tggcacagac aagtcaccat aaacgcccgt cagcaatgga tacgctgcgc tatctggcta
    11461 gaaatccgcg ctttcgtcgc ggtattgtgg cgcagttcct ttatgtgggt atgcaggtcg
    11521 cagtatggtc atttaccatc cgtctggcgc tggagttggg cgatatcaac gaacgtgatg
    11581 cttcaaactt catggtctat agctttgcct gcttctttat tggcaagttt atagccaata
    11641 tccttatgac gcgttttaat ccggaaaaag tattgattct ctattccgtt ataggggcgc
    11701 tgttcctggc atatgttgca ctggctccaa gtttcagcgc ggtctatgtc gccgtcctgg
    11761 taagtgtttt gtttggccct tgctgggcga cgatctatgc aggaacactg gatacagttg
    11821 ataacgaaca tacagaaatg gcaggagcag tcattgttat ggctattgtc ggtgccgcgg
    11881 tggttccggc aattcaggga tatattgcag atatgttcca ctcactacag ctttcattcc
    11941 tggtttctat gctctgcttt gtctatgtcg gagtctactt ctggcgtgaa agcaaagtac
    12001 gtaccgcact ggctgaagtc acagcgtcct gaggagaacc gttatgagta cgcgtattaa
    12061 tttgtggcgg gcgctttttg gcgagaaacc tcgtatcttg ctggaaaata gtgattttac
    12121 tgtcacgtct tttcgatatg acagcggtgt cgaggggctt aaaattgcaa acagtcgtgg
    12181 acatttgatt attcttccct ggatggggca gatgatttgg gatgcgcaat ttgatggaca
    12241 cggcctcacc atgtgcaata tgtttcgcca gcctaaaccg gcgacagagg tgatagagac
    12301 ctatggctgt tttgcttttc attcaggttt gctggctaat ggctgcccat ctgcggaaga
    12361 tactcatctg ctacacggtg aaatggcctg tgccgcgatg gatgaagcat ggatggaatt
    12421 agagggagat atgttacgcc ttaccgggcg ttacgagtat gtaaaggggt tcgggcatca
    12481 ttatctggct cagccaacag tggtccttca caaatcctct acactgttcg atattaagat
    12541 ggccgtaacc aatctggctt ctgtagatat gccactacaa tatatgtgcc acatgaatta
    12601 tgcatatata ccaaatgcta cgttcagtca gaatataccg gacgaaatat taaggttgcg
    12661 tgaatcagta ccttcgcatg ttaatcccac cactcagtgg ctggcgttta atcaacggat
    12721 tatgcaggga gaagcctctc tgtcgacgct gaatcaaccg gagttttatg atccagaaat
    12781 tgtcttcttt gcagacaaac tggacgctta cacagatcag ccagagtttc gcatgatcgc
    12841 tcccgatggc actacgtttg ttactcgttt ctccagtgcc gaactaaatt atgtgacacg
    12901 ctggatcctt tataacggag agcaacaggt agccgccttt gcattacctg caacctgtcg
    12961 gcctgagggg tatcttgccg cgcaacgtaa tggcacgctg attcaggttg caccgcagca
    13021 aacgcgcact tttacagtca ccacaggaat tgaataacca ctcaaggcct gaacagcggc
    13081 gcgcttatcg ttaaggtaag cgcgtttttt tacccgccag gacaagacta tgatagcgtt
    13141 gcccttatag acgatcacat tatcgtccac tccaaatttg cgcaactact gtagagtggt
    13201 gtactccaca gcttctttga ggagcttaac ctccattgtt ttcttaccga gcaagcactg
    13261 caactctttt atttatttgt agtggtcaac aaaaactggt cacaatttca gtttttttcc
    13321 agtatctatt ttccgattcg ttcggcggta atccaccgtt atattcatga ggccggagtg
    13381 cactataaaa gtgtatgcgt aaaccgactg ctgtatgtag ccattaagcc agtattggta
    13441 acataggtac ccaccttttc aactagtgga cacctgttat ggaacaaaaa acattatcag
    13501 ctgaaccccg caggtcattt tcaaatgagt ttaaacttca aatggttaaa ctggctttac
    13561 aaccaagggg ttctgttgcc cgtattgccc gggagcatga tatcaatgat aatcttctgt
    13621 tcaaatggct caggctctcg ctgatcaaag ggcgtatatc acggcgtctt ccggtaacga
    13681 actcttctgg cattggcgtt gaattattac ctgtggagat gacgctggat gagcggcctt
    13741 accccgtttt ttacgcgagg taattatgaa gatcaatgac tagccgtcga accgtgtgca
    13801 tgaactgctg cactgaaact tctcagctgt aaaataatcc ttacgctacg tacttcccgg
    13861 agcgcttacg attaatcgga tgcagtcctg actggaagaa agaaaaatgg agaaaataag
    13921 aggaattaat atcctccacg ttcctgaaat cctggaggat attaatgtca tcaaaatatt
    13981 agcgaacgat aatccctaac aaaataccca gtgcgccaaa gtcagcatca ctgaaggtgg
    14041 tgttagcaaa accaatatca cccagcaccg gcaataagaa tactggcagg aaggtaataa
    14101 gcaaaccctg agcgaaggca cccaaaatcg caccacgacg tccacccgtt gcattaccga
    14161 atacccctgc agctgcacct acgaagaagt gcggcaccac accaggaata atcacgatca
    14221 tgttcagcag atacaacgtg aacataccga ttaaacctgc agcaaagcta ctcaggaaac
    14281 caaccagtac agcgttaggt gcgtaaggga agacgaccgg gcaatcgaga gcaggtctgg
    14341 cattcggaac cagcttgtct gaaatacctt tgaatgccgg gacaatctcc gcaatcacca
    14401 tgcgcacacc ctgcagaatg atgtacacgc cagctgcgaa ggtaatggat tgcatgatag
    14461 agaacatgaa ccagttttta ccaccgctaa gctctttcac cgcatctgca cccgcaaaca
    14521 ggcacgtcac caggaaaatt atgctcatgg taaaagagat agcaaccggc gtgtcgcgca
    14581 agaacagcag acttttcggc acattcatgt cttcagtcga gtgctcttta ttaccaaact
    14641 tgctgccgat gaaaccggcc aatacataag ataatgttga gaagtggccg atggcaacgt
    14701 catcagaacc ggtcactttc ttcatgtacg gatgcgcaat agccgggaaa aagaccattg
    14761 ccacgccaac aaccaaagag ccaacggcta taagcgtaat gccggtcatg cctgctgttg
    14821 ccagaattac cgctaccatc atcgacataa acaacgtgtg atgaccggtc aggaagataa
    14881 atttccacgg tgtgaaacgg gcaatcataa tgttgataac catcgcaaag aacataatca
    14941 tcgccatttc tttcccgaag cttttctgcg ctacggaaac aatggcttca ttgttcggca
    15001 cta
//
LOCUS       DAZGAP010000137        14666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGAP010000137 DAZGAP010000000
VERSION     DAZGAP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.138"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..268
                     /locus_tag="ACHLAM_003672"
     CDS             <1..268
                     /locus_tag="ACHLAM_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO7855135.1"
                     /translation="TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLL
                     VFAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            278..916
                     /locus_tag="ACHLAM_003673"
     CDS             278..916
                     /locus_tag="ACHLAM_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO7855136.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            919..2082
                     /locus_tag="ACHLAM_003674"
     CDS             919..2082
                     /locus_tag="ACHLAM_003674"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7855137.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2166..3791
                     /locus_tag="ACHLAM_003675"
     CDS             2166..3791
                     /locus_tag="ACHLAM_003675"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO7855138.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERMEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3899..4174)
                     /gene="yjfN"
                     /locus_tag="ACHLAM_003676"
     CDS             complement(3899..4174)
                     /gene="yjfN"
                     /locus_tag="ACHLAM_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO7855139.1"
                     /translation="MKQFLASSSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4323..4652)
                     /gene="bsmA"
                     /locus_tag="ACHLAM_003677"
     CDS             complement(4323..4652)
                     /gene="bsmA"
                     /locus_tag="ACHLAM_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO7855140.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4834..5583
                     /gene="yjfP"
                     /locus_tag="ACHLAM_003678"
     CDS             4834..5583
                     /gene="yjfP"
                     /locus_tag="ACHLAM_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7855141.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHSTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5580..6335)
                     /gene="ulaR"
                     /locus_tag="ACHLAM_003679"
     CDS             complement(5580..6335)
                     /gene="ulaR"
                     /locus_tag="ACHLAM_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO7855142.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6443..7507)
                     /gene="ulaG"
                     /locus_tag="ACHLAM_003680"
     CDS             complement(6443..7507)
                     /gene="ulaG"
                     /locus_tag="ACHLAM_003680"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO7855143.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7862..9259
                     /gene="ulaA"
                     /locus_tag="ACHLAM_003681"
     CDS             7862..9259
                     /gene="ulaA"
                     /locus_tag="ACHLAM_003681"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7855144.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKFAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9275..9580
                     /gene="ulaB"
                     /locus_tag="ACHLAM_003682"
     CDS             9275..9580
                     /gene="ulaB"
                     /locus_tag="ACHLAM_003682"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO7855145.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            9590..10054
                     /gene="ulaC"
                     /locus_tag="ACHLAM_003683"
     CDS             9590..10054
                     /gene="ulaC"
                     /locus_tag="ACHLAM_003683"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO7855146.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10068..10718
                     /gene="ulaD"
                     /locus_tag="ACHLAM_003684"
     CDS             10068..10718
                     /gene="ulaD"
                     /locus_tag="ACHLAM_003684"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7855147.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10728..11582
                     /gene="ulaE"
                     /locus_tag="ACHLAM_003685"
     CDS             10728..11582
                     /gene="ulaE"
                     /locus_tag="ACHLAM_003685"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO7855148.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEISVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            11582..12268
                     /gene="ulaF"
                     /locus_tag="ACHLAM_003686"
     CDS             11582..12268
                     /gene="ulaF"
                     /locus_tag="ACHLAM_003686"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO7855149.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12397..12672)
                     /gene="yjfY"
                     /locus_tag="ACHLAM_003687"
     CDS             complement(12397..12672)
                     /gene="yjfY"
                     /locus_tag="ACHLAM_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO7855150.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13000..13395
                     /gene="rpsF"
                     /locus_tag="ACHLAM_003688"
     CDS             13000..13395
                     /gene="rpsF"
                     /locus_tag="ACHLAM_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO7855151.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13402..13716
                     /gene="priB"
                     /locus_tag="ACHLAM_003689"
     CDS             13402..13716
                     /gene="priB"
                     /locus_tag="ACHLAM_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO7855152.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13721..13948
                     /gene="rpsR"
                     /locus_tag="ACHLAM_003690"
     CDS             13721..13948
                     /gene="rpsR"
                     /locus_tag="ACHLAM_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO7855153.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13990..14439
                     /gene="rplI"
                     /locus_tag="ACHLAM_003691"
     CDS             13990..14439
                     /gene="rplI"
                     /locus_tag="ACHLAM_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO7855154.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         3390 a         3604 c         3990 g         3682 t
ORIGIN      
        1 caccgcggcg gcactggcgt tcagcggaac attgttgggg tacgtgatcc ccttatccag
       61 tgcggcaatc aatgcggtga gtattccaga ctatttcgca tggggcggga ttgctctggt
      121 gattcaatta ctcgtttttg ctggcgtcag gctttatatg cccgcattaa gcgaaaaaat
      181 tattaatcac aataccgcag caggaatgtt tatgggaacc gccgcgctgg ctggcggtat
      241 ttttaacgca gcttgtatga catggtaatg gacgatcatg gccagaaaac gcaaatccag
      301 aaataacaaa aatatcggac atggggcaat cagtcgcatt ggtagaccgc ataatccttt
      361 tgagccgcgc cgcaatcgct acgcacaaaa atacttaacg ttggcgctaa tgggcggtgc
      421 cgcttttttc gtattgaaag gttgtggcga tagtggcgat gtcgataacg acggcgacgg
      481 aacgttttac gcgactgtgc aggattgtat tgatgacggt aataatgcag acatttgcgc
      541 gcgtggctgg aacaacgcca aagcggcgtt ttatgccgat gttccgaaga atatgactca
      601 gcagaattgt cagtctaagt acgaaaattg ctactacgac aatgttgaac agagttggat
      661 cccggtcgtt tctggatttt tgttaagccg ggttattcgt aaagatcgcg atgagccgtt
      721 cgtctataac agcggcggat cctcatttgc ttcgcgcccc gtctggcgca acacttctgg
      781 tgattactcc tggcgctccg gttctggcaa aaaagagtct tactcttcgg gcggctttac
      841 caccaaaaaa gcgtcaaccg tttctcgcgg cggctatggt cgttcttcca gcgcccgtgg
      901 gcattgggga ggctaatcat gctgagacac aacgttcctg tgcggcggga tctggaccag
      961 atcgccgctg acaacggttt cgactttcat atcatcgaca atgaaatcta ttgggacgag
     1021 agtcgagctt accgttttac tctgcgccag attgaagagc agatcgaaaa accgactgcg
     1081 gaactgcatc agatgtgcct tgaggtggtg gatcgcgcgg ttaaagacga agagatcctg
     1141 acgcaactgg cgatcccgcc gttgtactgg gatgtgatcg ccgaaagctg gcgcgcacgc
     1201 gatccttcgt tgtatggccg tatggatttt gcctggtgtg gtaatgcacc ggttaagttg
     1261 ctggagtaca acgccgatac gccaacttca ttgtacgagt cggcttattt ccagtggttg
     1321 tggctggaag atgcccggcg cagcggcgtt attccgcgcg atgccgatca gtacaatgcc
     1381 attcaggagc gcctgatttc acgctttagc gagctttaca gtcgggaacc gttttatttt
     1441 tgctgctgtc aggacaccga cgaagacagg agtaccgtgc tgtacttgca ggactgcgcc
     1501 gagcaggcag ggcaggagtc gcggtttatc tacattgaag atctcggttt gggcgtcggc
     1561 ggtgtgctga ccgatcttga tgataatgtc atccagcgtg catttaagct gtatccgctg
     1621 gagtggatga tgcgtgacga taacggtccg ctgctgcgca agcgtcgcga gcaatgggtg
     1681 gagccgttat ggaaaagtat cttgagtaat aaagggctaa tgccgctgct ttggcgcttc
     1741 ttccctggtc atcctaacct tcttgcatcc tggttcgatg gtgaaaaacc gcagattgcc
     1801 gctggcgaaa gctatgtgcg taaaccgatt tactcgcgcg aaggcggaaa cgtcaccatt
     1861 ttcgacggtc agaataacgt tgttgaccac gctgatggtg attacgccga tgaaccgatg
     1921 atctaccagg cgtttcaacc tctgccgcga tttggcgata gctacacact catcggtagc
     1981 tggattgtcg atgatgaagc gtgcggaatg gggatccgtg aagataacac gttgatcacc
     2041 aaagacacct cacgtttcgt tccgcattac attgctggat aagaatgttt tagcaatctc
     2101 tttctgtcat gaatccatgg cagtgaccat actaatggtg actgccattg atggagggag
     2161 acacagtgca ctggcaaact cacaccgttt ttaatcaacc tataccatta aataacagca
     2221 acttatacct gtctgatggc gcgctctgcg aagcggtaac gcgtgaaggt gctggctggg
     2281 atagcgattt tctcgccagt attggtcagc agttagggac ggctgaatcc cttgaactgg
     2341 ggcggctggc gaatgtgaat ccgcctgaat tattgcgcta cgatgcccag gggcggcgtc
     2401 tggacgatgt gcgttttcac cccgcctggc acctgctgat gcaggcgcta tgtaccaatc
     2461 gggtgcacaa tcttgcctgg gaagaagacg ctcgctccgg cgcatttgtg gcgcgcgcgg
     2521 cgcgttttat gttgcacgca caggttgagg cagggtcgtt atgtccgata acgatgacct
     2581 ttgccgccac gccattgctg ttacagatgt tacccgcgcc gtttcaggac tggaccacgc
     2641 cgctgttgag cgatcgctac gattctcact tattgccagg tgggcaaaaa cgcggtttgt
     2701 tgattggcat gggaatgacg gaaaaacagg gcggttccga tgtcatgagc aacaccaccc
     2761 gcgcagagcg catggaagat ggctcttatc ggctggtggg gcataaatgg tttttctcgg
     2821 tgccgcaaag cgatgcgcat ctggtgctgg cgcagactac gggcggtttg tcctgctttt
     2881 ttgtgccgcg ctttttgcct gacgggcaac gcaacgcgat tcgcctcgag cggctgaaag
     2941 ataagctggg taatcgctct aacgccagct gcgaagtgga gtttcaggat gccattggct
     3001 ggttgttggg gcaggaaggg gaaggaattc gtctgatcct gaaaatgggt gggatgacgc
     3061 gttttgattg cgccctgggt agccatgcca tgatgcgccg tgcattttcg ctagcgattt
     3121 atcatgcaca tcaacgccat gtttttggta atccattgat ccaacagccc cttatgcgtc
     3181 atgtcttaag tcgcatggca cttcagcttg aagggcaaac ggcgttgctg tttcgtcttg
     3241 ctcgagcgtg ggaccggcgt gccgatgcca aagaagccct gtgggcgcgt ttatttacgc
     3301 ctgcggcgaa atttgtgatc tgcaaacgag gtatgccgtt tgtggccgaa gcgatggagg
     3361 tgctgggcgg cattggttat tgcgaggaga gcgagctgcc gcggctttac cgggagatgc
     3421 cggtaaacag tatttgggaa ggttccggta atattatgtg cctggatgta ctgcgcgtgc
     3481 tcaataagca agcgggtgta tacgacttat tgtcggaagc atttgtggaa gtgaaagggc
     3541 aggatcgcta ttttgatcgc gcggttcgtc gtttacagca gcagctgcgt aagccagctg
     3601 aagaactggg gcgagagatt actcatcagc tattcctgct gggctgcggt gcgcaaatgt
     3661 tgaaatatgc ctctccgcca atggcgcagg cgtggtgtca ggtgatgtta gatacgcgcg
     3721 gcggcgtacg gttgtcagag cagatccaga atgatttatt gctgcgggcg acgggaggag
     3781 tgtgtttgta agcgtatacg acgctggttt cgattaactc aatgaaatat gtgaaaattg
     3841 ttagccggac aaggcgctcg cgccgcatcc ggcattgttc atttgctgta gatgacactc
     3901 acgcatacag gatcgcctgt acgcgccagt tatcaggctg ctggtcttcg tacatggtaa
     3961 taatgcggta ccatgacgca ccttgttcat cagcctttaa cgccacgata cgttccacgt
     4021 cctgcggatc ccctgaaata ttgctaactg agatctgacc tatttcattc aggcctgtca
     4081 cgcagtcagc actggcgaat tctgcagact gtgccgtggc gctcgcagga taagtcacta
     4141 attgcagcga ggatgaggca aggaattgtt tcatcgtcag ctccattttt cgtttcccca
     4201 catgttgcag ggcattccat cgcggtaata aacgcccagg cttccagccg gtaatttatt
     4261 gtgcacaggt aaacgcatag ggacgcaaag caatgtaaag ctgactatgt aagttgtctg
     4321 atttatttac ggtacaaaat ggcttgtgaa taccactgtc ctgtgacgat ggtttcgtcc
     4381 accatgacga caacgtaata atcagctttt gcagcgacag ctttcgctct gatttctgct
     4441 aatgcgtcat ccggagaacc ccgaaccatc gtgcttacgc tacctattcg ctgtaaccct
     4501 tgcgtctggt cgcggcgaat ctcttgcgga tggtcggtta ctggcggtgc tggctgtggc
     4561 gtaccttgta gtgcgctaca ggcacttaac atcaacacca ataataaact ggcaaaccgg
     4621 taaataacgc tattacgttt cctgctaacc atagtgtagt gccttattaa tttaactttg
     4681 ggggaatgtt cccgaattgt tacctggtac gcgattttcg aatgaaaacg tgatgaaagc
     4741 gtaacccaca tctcaacatt atgaactaat accgccagca gaaacttgtg ctaaggattt
     4801 cttcatgtca taccagacaa aaaggagaga cagatgattg aaatagaatc acgcgagctg
     4861 gcagatattc ccgttcttca tgcttatcct gtcgggcaaa aagatacccc gttaccgtgc
     4921 gcgatttttt atcacggctt tacttcgtcc agtctggtgt atagctattt tgccgttgcg
     4981 ctggcgcagg ctggtttgcg ggtgatcatg ccggatgcgc ccgatcacgg tagtcgtttt
     5041 agtggcgacg cagcgcggcg gttaaatcaa ttctggcaaa tcttgctaca aagtatgcag
     5101 gaattcacta ctttacgtgc ggcaatagcc gaagaaaact ggctgcgtga tgaccgtctg
     5161 gcagtcggtg gcgcgtcgat gggcgcgatg acggcactgg gaattaccgc tcgccactcc
     5221 acggtgaaat gtaccgccag catgatggga tcgggctatt ttacatcgct cgcccgttca
     5281 ctgtttccac cgctgatacc tgaaacggca gcacagcaga atgaattcaa taacattgtc
     5341 gcgccgctgg cagagtggga agcgacaaac cacctggaac aacttgctga cagacctcta
     5401 ctgctgtggc atggccttga cgatgacgtc gtgcctgccg acgaatcact acgtttgcag
     5461 caggccttaa gcgagacggg acgggataaa ctgctaacct gttcatggca gccaggcgtg
     5521 cgtcaccgca ttacgcctga ggcgttagat gctgccgtga catttttccg ccagcatctt
     5581 taaacacgca gaatgctgac cccttgcgct tccagttgtt gcaggatttc cgggttagca
     5641 tttttgccgg tgatcagcat atcgatttga tcggcacggc taaaaagcat tcccgcgcgt
     5701 tcgccgatct tactgctatc aaccagtacc accagtttcc ctaccacgct tagcatcttc
     5761 tgctctgcca ttgccgtcag catatcggtt ttatacaacc cttctgctgt cagccctttg
     5821 ccgctggtaa acatccagtg cccggcatag agactgtttt cgctgccttg cgggcttaaa
     5881 gtgatggact gacttttgtt gtactgtccg cccataatga tcacgctgtc atgctcctga
     5941 tcgatcaggt aatttgccag cggtagataa ttagtgatga tttgcactgg cttgccacac
     6001 atttcccgcc caagcagaaa cgcggtggag ccgcagttga tgactacgct ttcgcccgga
     6061 ttaaccagct gcgaggccgc tttagcaata cgtacttttt catcgtgatt ctgcgcctga
     6121 tgcagattca tcggcgtcca gcgtgggcgc tgctgggtaa tagcttctgc gccattgcgc
     6181 acttttttca gtttgccgct ttcgtcaagt ttattgatat cgcgtcgcgc agtggcaggc
     6241 gaaattccca gacgctcaac gactttctca acggtcacaa agcccaattg tgcgagcatt
     6301 tccaggagga tttgatgtct ttgtgcttca gtcatgagct attccgataa aatttgattt
     6361 ttttagatga tatttgaaat agccaggaaa tactacgccg gatagcgcga actactccac
     6421 gctatccggt acaagcgaca gattacagga acgacttgaa cggcagatcc ggttcaatag
     6481 tgaagcaatc atcgaaacca cgcggatagt ggtactcgaa gttgtcttta tccagcggcc
     6541 aggtaaattt gccgccgacc tgccagataa acggcttgaa gccatacttc aggcgatctt
     6601 ttttcatctc ccacagcacg cggatctctt gcggatcggc ctggaagttt gaccagatat
     6661 cgtggtggaa cgggatcact actttagcat tcagcgcttc gcccatacgc aagatatcag
     6721 cactggtcat tttgtcggta ataccgcgtg ggttttcgcc gtatgaacct aaagccacgt
     6781 cgatctgatg ctcgttgcca tgttttgcat agtagttaga gtagtgggaa tcgccgctgt
     6841 gatacagaga accgcccggt gttttgaaca gatagtttac tgcgcgatcg tccatgccgt
     6901 ctggcagcac gccagccgct ttttgatccg ctggtagggt gatcagtgca gtacggtcga
     6961 acgcgtcaag ggcatgaatt tcaatgtctt tcacttttac gacatcgccc ggtttgacca
     7021 cgatgcaacg ctctttcggt acgccccagc caatccataa atccacgcag gttttcggtc
     7081 cgataaacgg tacgtcatca gcacagttct gcatcacggc agcagcgacg ttgacgtcga
     7141 tatgatcgtt gtgatcgtga gttgccagta ccgcgtcgat ctggcgaatc gcaaacggat
     7201 caagaacaaa cggggtggtg cgcaggtttg gctgcagttt tttcacgcca gccatgcgct
     7261 gcatctggtg accctgtttc atcagcgggt taccgtgact ttgtttgcca gtaccgcacc
     7321 agaaatcaac gcaaacgttg gtgccacctt ccgatttcag ccagatcccg gtgcagccta
     7381 gccaccacat cgcgaatgtg ccaggggcga cctgctcttg ttcaatttct tcattcagcc
     7441 agctacccca ctccgggaaa gtgctcagga tccaggattc tcgggtgata cttttcactt
     7501 tactcatcgc catttacctt catgatagtt caattcgaat caacatgtga ttggttttga
     7561 ttaatcctga cactattttt tcaggaaggc aatgaccatt ttttgacttt ttgccaggga
     7621 agttgttgtt gattttgagt gtggaaagat ttaatggaat gtgtaattca attaactaaa
     7681 tgaatttaaa tgaataatta tttcgttgtg tgaatcccac tctatccatg tggaattatt
     7741 tgcgggtcgc gtcacattta atcataaata atcttgttgt gattactttt gaaaattaga
     7801 gtgagtgcac aacattccgg gtgtgtggaa tacccggtta cctcttcttc aggagatcgt
     7861 tatggagatc ctctacaaca tctttaccgt gttttttaac caggtcatga ccaatgcccc
     7921 gttgttgctg ggtattgtga cctgtctggg ctacatccta ctgcgcaaaa gtgtcagcgt
     7981 tattattaaa ggcacgatta aaaccataat tggtttcatg ttgttgcagg caggatccgg
     8041 catcctcacc agcaccttca aaccggtggt ggcgaaaatg tccgaagtct acggcattaa
     8101 cggcgcaatt tccgatactt acgcgtcgat gatggcaacc atcgaccgca tgggcgatgc
     8161 ctatagctgg gtaggttacg cggtgttgtt agcgctggca ctgaacatct gttacgtgct
     8221 gctgcgtcgc attaccggta ttcgtaccat catgctgact ggtcacatca tgttccagca
     8281 ggcggggctg attgccgtca cgctgttcat ctttggctac tccatgtgga cgaccattat
     8341 ctgcaccgcg attctggttt cgctctactg gggcattacc tccaacatga tgtacaagcc
     8401 gactcaggaa gtgacggacg gctgcggttt ctccatcggt caccagcaac agtttgcatc
     8461 atggattgcc tataaatttg cgccgttcct cggcaaaaaa gaggaaagcg ttgaagacct
     8521 caaactgcca ggctggctga atattttcca cgacaacatc gtctccacgg cgattgtcat
     8581 gaccatcttc tttggtgcca ttctgctctc cttcggtatc gacaccgtgc aggcgatggc
     8641 aggcaaagtg aactggacgg tgtacatcct gcaaactggt ttctccttcg cggtggcgat
     8701 cttcatcatc actcagggtg tgcgcatgtt tgtggcggaa ctctctgaag cgtttaacgg
     8761 tatctcccag cgtctgatcc ctggtgcggt tctggcgatt gactgtgcgg ctatttatag
     8821 cttcgcgccg aacgccgtgg tctggggctt tatgtggggc accatcggtc agctgattgc
     8881 ggttggcatc ctggtcgcct gcggttcctc gatcctgatt atcccaggct ttatcccgat
     8941 gttcttctcc aacgccacta ttggcgtgtt cgctaaccac ttcggcggct ggcgtgcggc
     9001 gctgaagatt tgtctggtga tggggatgat cgaaatcttt ggctgcgtct gggcggtgaa
     9061 acttaccggt atgagtgcct ggatgggtat ggcggactgg tcgattctgg caccgccgat
     9121 gatgcagggc ttcttctcca tcggtatcgc ctttatggct gtcatcattg taattgcact
     9181 ggcttatatg ttcttcgctg gccgcgcact gcgcgcagaa gaagatgcag aaaaacaact
     9241 ggcagaacag tctgcttaat aaggagtttt gattatgacc gtacgtattc tggctgtgtg
     9301 tggcaacgga caaggcagtt ccatgatcat gaaaatgaaa gtggaccagt ttttaaccca
     9361 gtcgaacatt gaccacacgg taaacagctg cgcggttggc gaatacaaaa gcgagttgag
     9421 cggcgcggat atcatcatcg cttctaccca tattgcgggc gaaatcactg tgaccggcaa
     9481 caaatatgtg gttggcgtgc gcaatatgct ctctcctgcc gactttggcc cgaaattgct
     9541 ggaagtgatc aaagagcatt ttccgcagga cgtgaagtaa ggacgcgcca tgaaattacg
     9601 tgattcgctg gcggagaata aatccatccg cctgcaggct gaagctgaga catggcagga
     9661 cgcagtaaaa atcggcgttg acctgctggt ggcggcggat gttgtcgagc cgcgttacta
     9721 ccaggcgatt ctggatgcgg ttgaacagca cggtccgtac tttgtgctcg cacccggcct
     9781 ggcaatgccg cacggtcgcc cggaagaggg cgttaagaaa accggttttg cgctggtgac
     9841 gctgaaaaag ccgctggagt tcaaccacga agataacgat ccggtggata tcctcatcac
     9901 catggcggcg gtcgatgcca acactcacca ggaagtgggc atcatgcaga tcgtcaatct
     9961 gtttgaagat gaagagaatt tcgaccgttt acgtgcctgc cgtaccgagc aggaagtact
    10021 ggatctcatt gaccgcacca acgcggcagc ttaagaagga attgaaaatg tcattaccga
    10081 tgttgcaggt cgcgctggac aaccagacta tggatagcgc ctatgaaacc actcgcctga
    10141 ttgccgaaga agtcgacatt atcgaagtgg gtaccattct gtgcgtgggc gaaggcgtgc
    10201 gtgcggttcg cgatctgaaa gcgctttacc cgcacaaaat cgtactggca gacgccaaaa
    10261 ttgccgatgc gggcaaaatc ctttctcgta tgtgcttcga agctaacgct gactgggtga
    10321 ccgtgatttg ctgtgcggat atcaacacag ctaaaggtgc gctggacgtg gcaaaagagt
    10381 ttaacggcga cgtgcagatc gaactgaccg gttactggac ctgggaacag gcgcaacagt
    10441 ggcgcgacgc aggcattggg caggtggttt atcaccgcag ccgtgacgca caggccgcag
    10501 gtgtggcgtg gggcgaagcg gacattaccg cgatcaaacg tctttccgat atgggcttca
    10561 aagtcaccgt caccggtggt ctggcgctgg aagatctgcc gttgtttaaa ggcattccga
    10621 ttcatgtgtt tatcgctggt cgcagcattc gtgatgccgc ttctccggtg gaggccgcac
    10681 gtcagttcaa acgttccatc gctgaactgt ggggctaagg agcggatatg ttgtccaaac
    10741 aaatcccgct tggcatctat gaaaaagcgc tccccgccgg ggagtgctgg ctggaacggc
    10801 tgcaactggc aaaaacgtta ggcttcgatt ttgtcgagat ttcggtagat gaaacggacg
    10861 atcgcctgtc gcgcctcgac tggagccgcg agcagcgtct ggcgctggtg aacgcgattg
    10921 ttgaaaccgg cgtgcgcgtg ccgtccatgt gcctttctgc tcatcgtcgt ttcccgctgg
    10981 gcagcgaaga tgacgcagtg cgggcgcagg ggctggagat tatgcgcaaa gctatccagt
    11041 tcgcccagga tgtcggtatt cgcgtgatcc aactggcggg ctacgacgtt tactatcagg
    11101 aagccaataa cgaaacgcgt cgtcgtttcc gtgacggcct gaaagagagc gttgagatgg
    11161 caagccgcgc gcaggtgacg ctggcgatgg agatcatgga ttacccgttg atgaactcca
    11221 tcagcaaggc gctgggttac gcgcactatc ttaacaaccc gtggttccag ctctacccgg
    11281 atatcggcaa cttgtcggcg tgggacaacg acgtgcagat ggagttgcag gccgggatcg
    11341 ggcatatcgt cgcggtgcat gtgaaagaca ccaaaccagg cgtcttcaaa aacgtcccgt
    11401 ttggcgaagg tgtagtggat ttcgaacgtt gcttcgaaac gctcaaacag agtggttatt
    11461 gcgggccgta cctgattgag atgtggagcg aaacggcgga agacccggcg gcagaagtgg
    11521 cgaaagcgcg tgattgggtg aaagcgcgca tggccaaagc cggcatggtg gaggcggcat
    11581 aatgcaaaag ctaaaacagc aggtatttga agccaatatg gatctgccgc gctacgggct
    11641 ggtgaccttt acctggggca acgtcagcgc cattgaccgc gaacgcgggc tggtggtgat
    11701 caagcccagc ggcgttgcct acgaaaccat gaaagcagac gatatggtgg tggttgatat
    11761 gagcggcaag gtggtggaag gggagtatcg tccgtcttcc gacaccgcga cgcatcttga
    11821 actctaccgc cgttatccgt cgcttggcgg cattgtccat acccattcca cgcatgccac
    11881 cgcatgggcg caggcggggc tggctatccc ggcattaggc accacgcacg ctgactactt
    11941 ctttggcgat atcccctgta cgcgcgggtt aagcgaagaa gaagtgcagg gggaatacga
    12001 gctaaacact ggcaaagtga ttatcgaaac gctgggtgac gccgagccgc tgcatacgcc
    12061 gggaattgtg gtgtatcagc acgggccatt cgcctggggg aaagatgctc acgatgcggt
    12121 gcataacgcg gtggtgatgg aagaagtggc gaaaatggcg tggattgccc gcggcattaa
    12181 cccgcaactg aatcacatcg acagctatct catgaataaa cacttcatgc gtaagcacgg
    12241 tcccaacgcc tattacgggc agaagtaaaa cacgcgctgc ggagatttcc ttcttcggga
    12301 gataactggt ctaagtccgc agccgttttt caaaaaaagc cccctgaaaa gggggcaaag
    12361 caaactatgg caatgtttcg ttggttatac ctggtgctag cgataaatat ccgcgctggc
    12421 gtgcatattg ccgttactcc ccggttcccg catcagaatc acgtggtagt acgttgcgcc
    12481 ctgcgcatct gtctcttcat ttaatgcctg acgcgcttcg ctttcagtgg cgaaattatg
    12541 attgatataa atcacgccta agctttgcac atcgtccata tttctggcct ggtggttatt
    12601 aatttcaatg gctgcccatg tatttgcact tagcaaaagc acagccagaa gggctaaaac
    12661 acggctgaac atagatacct cctcgacggc tgactttgtg tgctctcctc ttgtggtgat
    12721 cttctgattt aattttaatc aatgataaag aagtggatgg tgaccatttc tgatgcagtt
    12781 gttcaaaaaa acaccatgat gaagtgtgat gaacttcaaa tcagcgtgtt agtacgattt
    12841 ggtgaaggat gagatttatt tgtcggctgg ccttgagttt agcggggtat gtcagtacta
    12901 taacgcgtca tttttcagcc gacctttaac acgttccttg cctccccggg attcggctga
    12961 cccagacagg aggctgaata atccgtaagg agcaattcga tgcgtcatta cgaaatcgtt
    13021 tttatggtcc atcctgacca gagcgaacag gttccgggca tgatcgagcg ctacactgct
    13081 gccatcactg gtgcagaagg caagatccac cgtctggaag actggggccg ccgtcagctg
    13141 gcttacccga tcaacaaact gcacaaagca cactacgttc tgatgaacgt tgaagctccg
    13201 caggaagtga tcgatgagct ggaaactacc ttccgcttca acgatgccgt tatccgcagc
    13261 atggttatgc gtaccaagca cgctgttacc gaagcatctc cgatggttaa agcgaaagac
    13321 gagcgccgtg agcgtcgcga tgatttcgca aacgaaaccg ctgatgatgc tgaagctggg
    13381 gattctgaag agtaatttct gatgaccaac cgtctggtgt tgtccggcac cgtgtgcagg
    13441 actccccttc gaaaggtcag tccatcagga attcctcact gccagttcgt gcttgagcat
    13501 cgttctgtgc aggaggaagc cggctttcac cggcaggcat ggtgccaaat gcccgttatt
    13561 gttagcggac acgaaaacca ggccattact cacagtataa cggtcggcag tcgcataacc
    13621 gttcaggggt tcatttcatg ccataaggca aagaacggac tgagcaaaat ggttttgcat
    13681 gccgagcaga ttgaattgat agattctgga gactagccat atggcacgtt atttccgtcg
    13741 tcgcaagttc tgccgtttca ccgcggaagg cgttcaagag atcgactata aagatatcgc
    13801 tacgctgaaa aactacatca ccgaaagcgg taagattgtc ccaagccgta tcaccggtac
    13861 ccgtgcaaaa taccagcgtc agctggctcg cgctatcaaa cgcgctcgct acctgtccct
    13921 gctgccgtac actgatcgcc atcagtaatc ggtcacggtc cattaatacg actttgagag
    13981 gataaggtaa tgcaagttat tctgcttgat aaagtagcaa acctgggtag cctgggtgat
    14041 caggtaaacg ttaaagcggg ctatgctcgt aacttcctgg taccgcaggg taaagctgtt
    14101 ccagctacca agaaaaacat tgaattcttc gaagcacgtc gcgctgaact ggaagctaaa
    14161 ctggctgaag ttctggcagc agctaatgct cgcgctgaga aaatcaatgc actggaaact
    14221 gttaccatcg cgtctaaagc tggcgacgaa ggtaaactgt tcggttccat cggtactcgc
    14281 gacatcgctg acgctgtaac tgcagctggc gttgaagtgg ctaagagcga agttcgtctg
    14341 ccgaacggcg ttctgcgtac cactggcgaa cacgaagtga gcttccaggt tcacagcgaa
    14401 gtattcgcga aagtgatcgt aaacgtagta gctgaataat tcgttattca acgagacgta
    14461 aaaacgccga ccagtggtcg gcgttttgct tttaagtgat aaaaaccgca gaaacagacg
    14521 ttccctccat aaatctcttg ctattccttt tataaaaagt tcgcagtacc catacctggc
    14581 ccccagcctc gccgctggta gttcgtgtca tgttttttac gcgtaaaaca cgacaaaagc
    14641 atcaatacag cgatcgtctc acttgt
//
LOCUS       DAZGAP010000138        14392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGAP010000138 DAZGAP010000000
VERSION     DAZGAP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.139"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..324)
                     /locus_tag="ACHLAM_003692"
     CDS             complement(97..324)
                     /locus_tag="ACHLAM_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7855155.1"
                     /translation="MEIENTPENIKRLRKKIGLTQKECAEMFGMTARSWRRKEEPTGT
                     VSGSKLSPVEFEYILLLAGEHPEYVLSKRDA"
     gene            complement(314..574)
                     /locus_tag="ACHLAM_003693"
     CDS             complement(314..574)
                     /locus_tag="ACHLAM_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855156.1"
                     /translation="MKMRKGDRQYYLNKEGDTFRLVKRVKTFSKSATLGKTKATVKTV
                     ADIIFREEEFGSIDFTQDGLREGDKEIISIMLKNIKRDENGN"
     gene            complement(765..3026)
                     /locus_tag="ACHLAM_003694"
     CDS             complement(765..3026)
                     /locus_tag="ACHLAM_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO7855157.1"
                     /translation="MRLWIAEKPELGKLIAQVLGNPQYFDGYIVCNNDTVSWAIGHLL
                     KLIPPELKTPEYKSWSKNTLPLQLRPLQYIVIPKTEKQFNVLSGLITRADMIIHAGDP
                     DEEGQLLIEEILSYCGNKAPVKRVLINDLNKDAVRQAMRNLQDNRAFYGLYQKALARS
                     AGDYLFGLNMTRAYTIAAREKGHNEILSVGRVQTPVLGLVVRRYEENRDYKKYDYWCV
                     QAEFIDTDTIISPMLVPPEDAPVDDKGRIISEQYCDEAIRSCKGQQASVSLAESEIKS
                     RRPPLPFSLLDLQVKMNRQYGFTAEKVLEITQKLREEYRAITYNRSDCRYLSEEQFSQ
                     SPATLAMLSSFFPELGKIFSHIDSGRKSRAFNDKKISAHTAIIPTPTPTGLDQMNEDE
                     MRVYKAIVAQYLVQFMPDKTFDSAVVEFNINGFLFRQRAIRPVSPGWSILLADRDEET
                     LPDCKLFDAITSLTKGQQLVCSSVNSRKETSTPPPLYTEASLLEDLRRVARYVEDPRI
                     RQLLLDRDEGKEEERGGIGTPATRGHILNVLQVRGFIAVKNKKVVPTELGLSFIHALP
                     EVMTRPDMTALWHEQQSMIETGEISIDDFLDELEQFIFQQVSDVDISGLAVAEYQCTC
                     GGRYVRRRTDKGFFWGCSKYPDCRNTVPDRGGVPDFSRSEFERKVRCPVCGSGMKISP
                     KAYNCSNERCGFRLWGTQFGKELTLAQATDLLMKGHTREIKGLKKKDGAKFDAVLNLN
                     RDGSVVPTFKKKK"
     gene            complement(3067..3300)
                     /locus_tag="ACHLAM_003695"
     CDS             complement(3067..3300)
                     /locus_tag="ACHLAM_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO7855158.1"
                     /translation="MENKYRKYPKSSKQEYLFQLRKCSKLSTLDKIIQRKAYTMNDID
                     FVEFLTAADHRLAEIVTGQLFDKIPKNVWKLIY"
     gene            complement(3350..3706)
                     /gene="ssb"
                     /locus_tag="ACHLAM_003696"
                     /pseudo
     CDS             complement(3350..3706)
                     /gene="ssb"
                     /locus_tag="ACHLAM_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(3718..4983)
                     /locus_tag="ACHLAM_003697"
     CDS             complement(3718..4983)
                     /locus_tag="ACHLAM_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7855159.1"
                     /translation="MGKLTLVEQALRLHKEYYGDECFGTIVKNYDYYPKVAEIFINRK
                     NKNTYSNLDIEPVASSGVVHTGSNPVMVKVGRTKKLMFKDGSGAFRIHLGKNEVVHIV
                     RYIHNSSIRMEYAIGTTKGLFMLHNLCEAGRVKLNRSLPRKGIWKIGDYQGIYYYGKS
                     RKEEIENALRFSGHPKFSELENDFNQFFSDIDYYTRYGQSGMRKILFTGPPGTGKTTI
                     AKALGAKYQDKYVFVYADNYFREVCYAAANKKIPLIIIAEEADELYRADAGTLSFLDG
                     ADTPRNPAGTYVIFSTNYPRRIDPRIRKRPGRIDRVISIGAFRTKDAASCAKMYLPEG
                     ININIKELGEVLDRTTPAEIKEIINISIGIIRGTKKELSTEIIKNARAFLKGSLDLSI
                     QEAEEDIDEREDIYRQNGAQPDYLSYLED"
     gene            complement(5001..5513)
                     /locus_tag="ACHLAM_003698"
     CDS             complement(5001..5513)
                     /locus_tag="ACHLAM_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO7855160.1"
                     /translation="MKITLTIFIFLLTTFQAANAGGIPGALAGILSTLNQEIQGKVIR
                     VLDGDTIEVLQDKKPVRIRLANIDAPEKKQAFGRWSANQLKALLAAQPVTVTYTQTDR
                     YGRIIGRVFTTNGTDASRFMVQSGAAWVYERYNTDKSLPALQREAQEQKRGLWADTNP
                     VPPWEWRKNN"
     gene            complement(5592..6167)
                     /locus_tag="ACHLAM_003699"
     CDS             complement(5592..6167)
                     /locus_tag="ACHLAM_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO7855161.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKEYPVKELPDIDESAHPF
                     YVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTHMDCHSIAYYDIHTLPLISCGR
                     QKIVPFAALIKADRCIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCEHR
                     FRQPVGEDNWKQAISEECAIRYANRLIQCEG"
     gene            complement(6284..6490)
                     /locus_tag="ACHLAM_003700"
     CDS             complement(6284..6490)
                     /locus_tag="ACHLAM_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855162.1"
                     /translation="MSEIRFVTLSSDTDINKIIQEGLTQKDATTTIVLGCASEGKEAP
                     LRLIETCFRTIPKHISIEEINEIK"
     gene            complement(6503..6892)
                     /locus_tag="ACHLAM_003701"
     CDS             complement(6503..6892)
                     /locus_tag="ACHLAM_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855163.1"
                     /translation="MDKSDEEKIGYILDEHERIFRKQASLYTVLGILNLIICLLCVVA
                     TFCVSVILPESHLKYQLFSFTILCMVCSFIIYYYTRHMVLNRQELLRLMRLTEGAPDV
                     KQELLNRLLSGKILTYWDEKDIRRLIF"
     gene            complement(7020..7331)
                     /locus_tag="ACHLAM_003702"
     CDS             complement(7020..7331)
                     /locus_tag="ACHLAM_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO7855164.1"
                     /translation="MKYSVIAVIILFLLSSKSYAEEVSIDSGDPCTVFMCMAGKVYGE
                     NSSECKGPTKKFFNIIKKKKGRIRWSKTFDARKAFLMNCPGADPAHVSKIMSKFGRKL
                     F"
     gene            complement(7356..8984)
                     /locus_tag="ACHLAM_003703"
     CDS             complement(7356..8984)
                     /locus_tag="ACHLAM_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO7855165.1"
                     /translation="MGKQKIPDGWVLENGKLIQKRVTHYPPIIFGKDPYSNNLLVSYG
                     QTYLMLAAPPGSGKGVGIVIPNLLLYPHSVVVNDCKFENWDLTAGFRKACGQKVVRFS
                     PERLETHHWNPLKVINQDPLYRLGDVRTLASSLYVPDNMKNASWFSKAADVFTAIVLY
                     LIETPSLPLSLPQCYEIASLGTRIGEWAQRVIQARVGTPDALSEETVRELGVIISESK
                     GREFSTLMSFVTGRLSLYGEKLVALALTESDNEEENIDFSKLREEPTSIYFCVTEGAL
                     QKFGPLMNLFFSQAIRENSKILPEQGGNLEDGSLRLKYQVLFLEDEFAVMGRIKVMET
                     APALSRGAGLRYIIVFQNKSQVCSDDCYGPAGGKAVMDPFHIEVVFAPGDISTAEEYS
                     KRLGTYTERVASSSRNISEGQRRSRGRNVTLHARPLMLPQEVNELPYPEELVFMQPTK
                     WNPALKGRVKKIMWYKEPELTARVDPVRYPVPDIPAGNISKLKGLVVPISQYITDAVK
                     ISSPEGDYLNEEQKKRLKIPQVESERESYEDNDY"
     gene            complement(8984..9346)
                     /locus_tag="ACHLAM_003704"
     CDS             complement(8984..9346)
                     /locus_tag="ACHLAM_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855166.1"
                     /translation="MEKLKLFIVIIALCCLFFVIGLYAGGWMWLELSGVDSSPSLMTL
                     ISQGGLPALNDRARMLLPWAWCVTVAITFFPAGVSLLAMTHGADGNRKDLHGNARFAG
                     KRELRRFWYEGPEKEDRN"
     gene            complement(9358..10407)
                     /gene="virB11"
                     /locus_tag="ACHLAM_003705"
     CDS             complement(9358..10407)
                     /gene="virB11"
                     /locus_tag="ACHLAM_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFO7855167.1"
                     /translation="MNTEFDLDKSELVLGWLDQSGVTERLSRQGVTEVAINRPFEIWT
                     ETSKDGWQREDAPWLTLNMCEHLAETLAVYNNRILGVKSPIKTVELPGGERGQIVIPP
                     ACEKSTVSMTFRKPSLQRFSLENYDSSGRFDNANRVRGRSKELLDWQKELKRLNAARE
                     YVEFFRLAVRHKLNIIIFGGTGSGKTTFAKAIVDVYPSNRRLLTIEELNEMKLPYHLN
                     HVHLLYGDYVTPKDLVACCMRMKGDHILLAELTGDETWDFMEVLNTGHPGTVTTAHAN
                     SSLHGYARISGLIKQSAVGAGLDISYIDRRVRTSFDVVMYMEKTEILDITYEPEVQLA
                     LLNGASMESALSFAS"
     gene            complement(10618..11973)
                     /locus_tag="ACHLAM_003706"
     CDS             complement(10618..11973)
                     /locus_tag="ACHLAM_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO7855168.1"
                     /translation="MTENVVTPPENETKKETSAADENKPQGQGVGAFSLKPAGRKKPV
                     NWKIVGVIIGGIVIVLLFTIIFAAIVINNRADPEPEIDETGIKAEVVTRSIGVIDDPM
                     GAFMEAHLPQDRDKTESDSRTVQSSGKQESNGPVSDKKNTQTSGGQSTVNPVTPVTDI
                     RVPETLFSPVARFDSSGISQGVGGSSDGVTSSSGYDVSAEEARLREFANADPAEIVNR
                     ALQSQADTVNGGISSDMRSNILGDLSSSRQYATAKAYKSPPRKYLLKRRTNFQCVLYT
                     AVKTDHSGFVGCRLTKPIFSADGSVILAEAGAELNGEQKIEVKQGQSTVFTSWSELET
                     TAGTEGGIRADLNGLGTGPMGSSGTDAYIDNHYGQRFGGAVMLSFIQDAIATAANAAQ
                     KNKSTYSFDNSEDNAESMAEKALENSINIPPTGYVLPGTVINVIVAQDIDFSSVYTVK
                     Y"
     gene            complement(11973..12785)
                     /locus_tag="ACHLAM_003707"
     CDS             complement(11973..12785)
                     /locus_tag="ACHLAM_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO7855169.1"
                     /translation="MRKIIKYIVPVCLIFSVTGFVHAEIAGKGSVSDKRIQTALYSPD
                     NVFRIYAMKDRTTMIQLEQGETINTDAGAMNLGKPGTEGAPEWLLGANKAGSLIMIKP
                     SRYAEDPRTNLIVSTNRRTYLFELHLTQSPALATYLFRFNYPKPPEVGKNPFVGRNIN
                     ENPCDGIINKKYQKRGDMIISPYQVWDNGTFTCFRFPTNAPRPVIYEVLPDGTETMVN
                     ARPVNDILVVHGVSREFRIRLNRLVLAVRSNVENTGWYNYKGTTTGEVREVK"
     gene            complement(12769..13632)
                     /locus_tag="ACHLAM_003708"
     CDS             complement(12769..13632)
                     /locus_tag="ACHLAM_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFO7855170.1"
                     /translation="MLFKSNKIKKETEQERLLHYGDGKLSGKSDEIGREIRAYTMAAR
                     WFEKRVAEDYRKKARNSRILAGLFGVIAIVAVIAVANLAPLKTVEPFVIRVDRNSGYM
                     DVVRPGWSKEDTKDISDDKHFISLYILSRETYNWASQKSNFAIVRQLSYSDVFSEYRN
                     FQLSGKGYVSTLNQSRQISVDIDSIVPLPVSTDKKLGERDDIKTYQVRFRQSLLDAEG
                     RPVPETGQILKLDENGKPIVKPKVVFWTAIISFDYLNTADTEGEAWLNPKGFGVLAYT
                     KTEEIRNEENN"
     gene            complement(13849..>14392)
                     /locus_tag="ACHLAM_003709"
     CDS             complement(13849..>14392)
                     /locus_tag="ACHLAM_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO7855171.1"
                     /translation="VSSIKSMVTVGVAVVLYLLSGLPFLGVAIATLLTLKVATNLILI
                     IGPMFIACLAFPKVAHWFWGWVGVLGGFVLTQALFAITLGMAISFINSNMINNGDFNI
                     SMVDIICMSLYFSAFTLLATEIPNYAASIMGGAPSGGVGSMSRLLGKATGFGAASHMS
                     KALGSKLVDRFHGRGKGRIS"
BASE COUNT         4130 a         3427 c         2358 g         4477 t
ORIGIN      
        1 ccgctgtgcg gctctggtga ttggggccat cccctctcga tgtattaata ataggtcata
       61 aacgacctat ttgcaagtta tttttcttct ttttttctag gcatcccgtt tagaaaggac
      121 gtattcagga tgctccccag ccagtaacag aatgtattca aactctacag gtgaaagttt
      181 tgatccgctt accgttccag tgggttcttc tttgcgccgc catgaacggg ctgtcatccc
      241 gaacatctct gcacattctt tctgcgttaa tccaattttc tttcgcagac gcttgatatt
      301 ttctggtgta ttttcaattt ccattttcat ctctttttat gttcttaagc ataatagaaa
      361 ttatttcttt atcgccctct cttaagccgt cttgagtaaa atcaatagag ccaaactctt
      421 cttcacgaaa aataatgtct gccactgttt ttaccgttgc ttttgttttc cctaaagtcg
      481 cagacttaga aaaagttttt acccgcttca ccagacgaaa cgtatcacct tctttattca
      541 gataatattg cctgtcacct tttctcattt tcatatctat atgattctct ctaagagaag
      601 cctcaagcgg agccgaacga ctcgtacact gtacgcatgg tttccctatt gcaacagatt
      661 taacatactt ttccatcttc aaactcctgc cgctgtgcgg ctttggtgac agagaactat
      721 cccttctcga tgcaaaggta ggtcataatc gacctatttg caagttattt tttcttttta
      781 aatgtcggta caacgcttcc atcacggtta agattcagca cggcatcaaa cttagcacca
      841 tcttttttct taagaccttt aatttcccgc gtatggcctt tcatcaacaa atctgtagcc
      901 tgtgctaagg tcagttcttt accaaactga gtaccccaca atcggaatcc acatcgttca
      961 tttgaacagt tataggcttt tggtgatatc ttcatcccgg aaccacatac cgggcatctg
     1021 acctttctct caaactcgct acgcgaaaaa tcaggcacgc cgcctctgtc tggaacagta
     1081 ttccgacaat caggatactt actgcatccc caaaaaaaac ctttgtcggt tctccgacga
     1141 acataacggc caccacacgt acactgatac tctgctacag ccaggccaga aatatccaca
     1201 tcagacacct gttgaaaaat aaactgttca agttcatcaa gaaaatcgtc gatactgatt
     1261 tcccctgtct caatcataga ttgctgctca tgccatagag ccgtcatatc tggtcgcgtc
     1321 atgacctccg gtaaagcatg aataaacgat aagcctaact ctgtaggaac cactttttta
     1381 ttcttgacag caataaaacc gcgaacctga agtacgttaa gaatatgccc ccttgttgcc
     1441 ggtgttccaa ttccccctcg ttcctcctct ttaccttcgt ctctgtccag taaaagttgt
     1501 ctaatcctgg gatcttcgac ataacgggcc acacgccgta gatcctcaag caatgatgct
     1561 tctgtataca atggtggcgg tgtggatgtc tcttttcgag aattcacact actgcaaacc
     1621 aattgttgac cttttgtaag gctggttatg gcatcaaata acttacagtc cggcaaggtt
     1681 tcttcgtctc tgtctgctag taatatactc cagccagggg aaacaggccg tatcgctcgt
     1741 tgccggaaca gaaatccatt gatattaaat tcaactaccg cagaatcaaa agttttatca
     1801 ggcataaact gcacaagata ctgtgccaca atcgccttat aaaccctcat ttcatcttcg
     1861 ttcatttgat ccagcccggt aggcgttggt gtcggaataa ttgctgtatg agcacttatt
     1921 ttcttatcat taaatgccct gctttttcgc ccactatcta tatgagaaaa aatttttccc
     1981 agttcaggaa aaaaagaaga aagcattgct aatgtcgccg gagactgaga aaactgttct
     2041 tctgagagat aacggcaatc cgaacggtta taagtgatag ccctgtattc ctcccttaat
     2101 ttttgcgtta tctccaacac tttttcagca gtgaatccat actgacgatt cattttcacc
     2161 tgaagatcaa gaagcgaaaa tggtaatggt ggtctcctgc ttttaatttc actctctgca
     2221 agagaaacac tcgcttgttg tcccttacaa ctacgaattg cttcatcaca atattgctcg
     2281 ctgataattc tacctttatc gtccacaggt gcatcttccg gtggaacaag catcggtgaa
     2341 attattgtat cagtatcaat aaattcggcc tgaacacacc aataatcata tttcttataa
     2401 tccctgtttt cttcataccg ccgaacgacc agtccaagaa ctggtgtctg aacccgtcct
     2461 actgaaagta tttcattatg ccctttttct ctggctgcaa tagtgtatgc tctggtcata
     2521 tttaaaccaa aaagataatc acctgcacta cgggcaagag ctttctgata aagcccgtaa
     2581 aacgctctgt tatcctgtaa attacgcatc gcctgacgca ctgcatcttt attaagatca
     2641 ttaatcagaa cccgtttaac gggagcttta tttccacaat aagaaagtat ctcttcaata
     2701 agtaactgcc cttcctcatc tggatcccct gcatgaataa tcatatctgc acgagtaatt
     2761 aatccagaca aaacattaaa ttgtttctct gtttttggga taacgatata ttgcaacggt
     2821 cgtaattgca gtggtaatgt atttttagac cagcttttat attcaggagt tttcaattca
     2881 ggtggtatta gctttaacaa atgaccaata gcccaagaaa ccgtatcatt gttgcaaact
     2941 atatacccat caaaatactg tgggtttccc agaacctgag caataagctt cccaagctct
     3001 ggtttttcag ctatccagag tcgcataaga atatctccgc ttgaaaagta cgacaggatg
     3061 tatgatttaa taaatcagct tccagacatt tttaggaatt ttatcaaata attgtccagt
     3121 tacgatttca gccaaccggt gatctgcggc tgtcaaaaac tctacaaaat caatatcatt
     3181 cattgtatat gccttacgct gaataatttt atcaagagta gaaagctttg agcacttcct
     3241 gagctgaaac aaatactcct gcttactact ttttgggtac ttcctgtatt tgttctccat
     3301 ttaagttatt cctcaaacgg actcaatatc ttcaagactg tcctgttttt gatttagtat
     3361 tattaccaag catttgcata gttcccttct gatcaacaac aatctctgtt gtataatgct
     3421 cctccccatc tttatctgtc cattttctgt aacgtaatgt tccctcaata tagacctgca
     3481 ttcctttaca taaaaaatct ctggatatct ctgccagttt gttataaata acaactcgat
     3541 gccattctgt attctctttt atttcaccag actgtttatc tctccacgtt tcgcttgttg
     3601 ctatacttaa attagcaacg gtaactccat tctgcataca ccgaatctca ggatcttgtc
     3661 caagattacc tattaatgta actttattta tacctttagc agccatttaa taccccacta
     3721 atcctcaaga taacttaaat aatctggttg tgccccgttc tgtctgtata tatcttccct
     3781 ttcatcaata tcctcctccg cttcctgtat acttagatca agagatcctt ttaaaaatgc
     3841 acgagcattt ttaattattt ctgttgataa ctctttttta gttccgcgaa ttattccaat
     3901 tgatatattt attatttctt taatttcagc aggtgtagtt ctgtcaagta cctcgccaag
     3961 ctctttaata ttgatgttta taccttctgg taaatacatc tttgcacaac tagcagcatc
     4021 tttagtcctg aaagcaccaa tagaaataac tctgtcaatt cttcccggac gtttcctgat
     4081 acgaggatca attctcctgg gatagtttgt tgagaatatt acatatgtcc cggcaggatt
     4141 tctcggcgta tcagccccat ccagaaaact tagcgtacca gcatcagctc tataaagctc
     4201 atcagcctct tcagctataa taataagagg tattttcttg ttcgctgcgg cataacaaac
     4261 ttctctaaaa tagttatcag catatacaaa tacatactta tcctgatact ttgccccaag
     4321 agctttggct attgtcgttt tacctgttcc tggtgggcct gtaaacagaa tcttcctcat
     4381 tccagactga ccataacgcg tatagtaatc aatatcagaa aaaaactgat taaagtcgtt
     4441 ctccagttca gaaaatttag gatgacctga aaaacgtaag gcattttcaa tctcttcttt
     4501 tctagattta ccgtaataat aaatcccctg ataatcacct attttccaga taccttttct
     4561 tggtaacgaa cgatttagtt taacgcgccc cgcttcacaa agattatgga gcataaacaa
     4621 acctttagtt gttcctatag catattccat tctaatactg gaattatgaa tatatctgac
     4681 aatatgcacg acttcatttt tccccaaatg aattctgaat gctcctgacc cgtctttaaa
     4741 catgagtttt ttagtccttc ccactttaac cattactgga ttacttcctg tatgaacaac
     4801 tccagaagaa gctacaggtt cgatatcaag atttgaatat gtatttttat ttttcctgtt
     4861 tataaatatt tctgctactt taggataata atcataattt tttacaatag tgccaaaaca
     4921 ttcatcacca taatattctt tatgaagccg caaagcttgc tctaccaaag ttaatttccc
     4981 catatgttac ctttaataaa tcagttgttt ttccgccatt cccacggtgg aaccggattt
     5041 gtatcagccc ataaaccacg cttttgttcc tgcgcttccc gttgcaaagc cggtaatgat
     5101 ttatctgtgt tgtaacgttc atatacccat gcagcgccag actgaaccat aaagcggctg
     5161 gcatccgtgc cgttcgtcgt gaacacacga ccgataatgc gtccgtagcg gtctgtctgc
     5221 gtgtacgtca cagtaacagg ctgtgctgca agcagagctt taagctgatt tgctgaccag
     5281 cgcccgaaag cctgtttttt ctccggtgca tcgatgtttg ccaggcgaat cctgacgggc
     5341 tttttgtcct gtaaaacttc gatggtatcg ccatcgagaa cacgaataac tttcccctgg
     5401 atttcctggt ttaatgtaga aagtattcct gctaaagcac caggtattcc acctgcattt
     5461 gcagcctgaa aggtagtaag cagaaaaata aatattgtta atgtaatttt cacaaaatac
     5521 taaccccctc cggttgttca actccgcaaa tcccagatta aaatcaggaa tctgcggagt
     5581 ttatgatttg cttatccttc acattgaatt aatctgtttg catagcgaat agcacattct
     5641 tctgatattg cctgtttcca gttatcttca cctacaggtt gtctgaacct gtgttcacag
     5701 ccattaaaag caaaaatccc ctcgcgatta agtatgttgg ttgcaatatc ccactcttta
     5761 atccttaaaa atgccgttac tgcaaaacca gaatatgatg caattttcga aatgatgcat
     5821 ctgtcagctt ttattaacgc ggcaaaagga actatctttt gacgtccaca cgaaatcaga
     5881 gggagtgtgt gaatatcata ataagctatt gaatggcaat ccatgtgagt atcatcaata
     5941 gctgccagcc agaatcctgt ctggtccttt atttttctgc taatatcaga atctgaaaca
     6001 ccaggattta ataaatatcc accataacag acatagaatg ggtgagcaga ttcatcaata
     6061 tcaggaagct cttttaccgg atattctttc cagtccttca gaacaccgaa ctcaagagca
     6121 tccagcaaaa actttcgttc aaatccatca aacaatgcat attgcatata ctacccctca
     6181 cagaatgcct gatttatata actaatgcgc tatcaataga tggctgtctc ttaaatataa
     6241 tgagattcga taataccaga ataacaacga ctaccgtcat cattcatttt atttcattga
     6301 tttcttcaat tgaaatatgc tttggtatag tcctgaaaca agtctctatg agcctgagcg
     6361 gtgcttcttt accctcactc gcgcaaccca acacaattgt agttgtagca tctttttgag
     6421 taagaccttc ctgaataatc ttgttaatgt cagtgtcaga tgacagagtt acaaatctga
     6481 tttcgctcat atgattacct cttcaaaaaa ttagacgtcg gatatctttt tcatcccaat
     6541 aagtcagtat tttcccggat agcagacggt taagcagttc ctgtttcaca tctggtgcgc
     6601 cctctgtcag acgcataagt cgaagcagtt cctgacggtt caggaccata tggcgggtgt
     6661 aataataaat aataaaactg cataccatac aaaggatagt aaaggaaaat aactggtatt
     6721 tcagatgtga ttccggcaat ataacagaaa cacagaacgt ggcaacaaca caaagaagac
     6781 atataattaa attgagtata cccagtaccg tatataaaga agcctgtttt ctgaatatcc
     6841 gctcatgttc atccagaata taacctattt tttcttcatc tgatttatcc atatatttca
     6901 ttcccatagt tgatgtggaa atagttattc ctctccgcaa atctaggata aacatatgtc
     6961 atctgcggag aatagttcaa acgcctctag tttagttcta aagcctttga ttattttatt
     7021 caaaaaagtt ttcttccaaa cttactcata attttactga cgtgagcagg atctgcccca
     7081 gggcaattca ttaaaaatgc ttttctcgca tcaaaagttt tactccaccg aatacgccct
     7141 ttcttctttt ttataatatt aaagaatttt tttgttggtc ccttacattc agatgaattt
     7201 tccccataaa cttttccagc catacacata aagacagtac agggatcccc cgaatctata
     7261 cttacctctt cagcataact tttactggat aacaggaaaa gaataatcac cgctatgaca
     7321 gaatatttca tattaacccc ctttttatta aaaattcagt aatcattatc ttcataagat
     7381 tccctttctg attccacttg tgggattttt aatcgttttt tctgctcctc atttaaataa
     7441 tcaccttctg gtgaagatat cttaactgca tccgtaatat actgacttat tggtacaaca
     7501 agtcctttaa gtttcgatat attcccggct ggtatatcag gcacgggata acgaacagga
     7561 tccactcttg ctgttaattc aggttccttg taccacatta tttttttcac ccggcctttc
     7621 aaggcgggat tccattttgt tggctgcata aacaccagtt cttcaggata tggaagttca
     7681 ttaacctcct gtggcaacat taaaggccta gcatgcagtg taacattacg tcctcgagaa
     7741 cgacgctgtc cttccgatat attcctgcta ctggacgcaa ctcgttctgt gtacgttccc
     7801 agtcgtttac tgtactcctc cgctgtactg atatctccgg gggcaaatac aacctcaata
     7861 tggaatggat ccattacagc tttcccccct gcgggtccat aacagtcatc agagcaaacc
     7921 tgacttttat tctgaaaaac aattatatag cgtaatcctg cccccctgct tagtgccggg
     7981 gctgtttcca taaccttgat acggcccatc acagcaaatt catcttccag aaaaagcacc
     8041 tgatatttca atctgagaga accgtcctcc agatttcccc cttgctccgg tagtatttta
     8101 ctgttttccc ttattgcctg agaaaaaaac aaattcatta gtggaccaaa tttctgtaaa
     8161 gctccctcgg taacacaaaa ataaatgctg gttggttctt cccttaactt gctgaagtca
     8221 atattttctt cctcgttatc actctccgtc agcgccagcg ccaccagttt ttcgccgtac
     8281 aagcttagac gtcccgtgac gaatgacatc aacgttgaaa attccctgcc tttcgactcg
     8341 gaaataatca cccccaactc tcggacggtt tcttctgaca gtgcatctgg cgttcctaca
     8401 cgagcctgaa taacacgctg cgcccattct ccaatacgag ttcccaacga tgcaatttcg
     8461 taacattgcg gtaaactcaa tggcaacgat ggtgtttcga tcaggtataa aactatcgcg
     8521 gtaaacacat ctgcggcttt agaaaaccaa ctggcattct tcatgttatc aggtacatac
     8581 agagatgagg ctagtgttcg aacatctccc aagcgataca gcggatcctg atttattacc
     8641 tttaacggat tccagtgatg ggtttccagt cgctctggcg aaaaacgcac gactttctga
     8701 ccacatgctt ttctgaaacc cgctgtcaaa tcccagtttt caaatttaca gtcattcact
     8761 acaacagaat gcggatacaa tagtagatta gggatcacaa tccccacccc tttaccactt
     8821 cccggtggag cggccaacat cagataggtc tgtccatagg atacaagtaa attattactg
     8881 tacggatctt taccaaaaat aattggtgga taatgagtta ctcttttttg gattaacttt
     8941 ccattttcca gcacccatcc gtctggtatt ttttgttttc ccattaattt ctgtcctcct
     9001 tctctggccc ttcataccag aaacgacgca attcccgttt tccggcaaac ctagcattac
     9061 catgtaggtc ctttctgttg ccgtccgctc catgagtcat ggccaaaaga gaaacacctg
     9121 ccgggaaaaa ggtaattgca actgtcacac accacgccca gggaagtaac attctggccc
     9181 gatcattcag tgctggcagt cctccctgac ttatcagggt catcaaagat ggagaggagt
     9241 caacacctga taattcaagc cacatccatc ctccagcgta aagcccaata acaaaaaaca
     9301 aacaacaaag agctattatc acgataaata gtttgagttt ttccatacag cactacctca
     9361 cgatgcaaaa gaaagagcac tttccatgct ggcaccattc agtaatgcca gttgcacctc
     9421 tggctcatac gtaatatcca agatttctgt tttctccata tacatcacaa catcgaaaga
     9481 cgttctgaca cgtcgatcga tataagagat atccagcccg gcaccaaccg cagactgctt
     9541 tattagtcca gagatacgag cataaccatg taaactgcta ttagcatgcg ctgtcgtaac
     9601 cgttcctgga tgcccggtat tcagcacttc cataaaatcc caagtttcat cgcctgtcag
     9661 ttctgccaga agaatatgat cccccttcat gcgcatacaa cacgctacca ggtcttttgg
     9721 tgtcacataa tctccataca ataaatgtac gtgattaaga tgataaggca gcttcatttc
     9781 attcaattct tcaatagtca gtaaccgacg atttgacggg taaacatcaa caattgcttt
     9841 agcaaaagtt gtttttcccg aaccggttcc accaaaaata ataatattta gtttatgtct
     9901 cacagccaga cggaaaaatt caacatattc acgagcagca ttaagacgtt ttaattcctt
     9961 ttgccagtcc agtaactctt tacttcttcc tcttacacga ttggcattat cgaaacgtcc
    10021 actggagtca taattctcga gagagaaacg ttgaagagac ggtttacgaa acgtcatcga
    10081 cacagtagat ttctcacagg cgggaggaat aacaatttgt cctcgttcgc ctcctggtag
    10141 ttccactgtt tttatcggtg acttaacacc taatatacgg ttattataaa cagccagtgt
    10201 ttcagccagg tgttcacaca tattcagcgt taaccacggt gcatcttccc tttgccagcc
    10261 atcttttgat gtctcggtcc agatctcaaa aggtctgtta atcgctacct ccgtcactcc
    10321 ctgacgggac agacgttctg taacaccaga ctgatccagc caccccaaaa caagttctga
    10381 cttatcgagg tcaaattcgg tattcattaa tagacgccct ttttatttac acttttatga
    10441 gttacttttc tatctcatta atgaaatgat aaatgcagca acgatcatta tgattgacca
    10501 ccgtaaattt attgggatac gataaaaata aatacacacc catttaaaaa atcccatcca
    10561 acctccttca agcaattact ttaatatata gccacccgtt aaggcggcta tatatcatta
    10621 atatttaact gtatagactg acgaaaaatc aatatcctga gcaactatta cattaataac
    10681 tgttccaggt aatacatatc ctgtaggtgg tatatttata gaattttcca gtgctttttc
    10741 agccatactt tccgcattat cctcactgtt atcaaaacta tacgttgatt tatttttttg
    10801 tgccgcgttt gcagctgttg ctatcgcgtc ctgaataaac gatagcatca cggctccacc
    10861 aaacctctgc ccataatgat tatcaatgta tgcatccgtg ccactgcttc ccatcggccc
    10921 cgtccctaat ccatttaaat ctgctcgtat acccccttct gtcccggcag tagtttcaag
    10981 ctcactccat gaagtgaaca ccgtagattg cccttgtttt acttcaattt tttgttcccc
    11041 attcaattca gcacctgctt cagccaaaat aacactaccg tccgctgaaa aaatgggttt
    11101 tgttaaccga caaccaacga atccagagtg atccgttttc accgccgtat aaagtacgca
    11161 ctgaaaattt gttctccttt tcaggagata cttacgtgga ggcgatttat aagctttagc
    11221 tgttgcatac tggcgggatg atgagagatc ccccaggatg ttagatctca tatccgaaga
    11281 tattccccca ttcacagtat ctgcctgact ctgtaaagca cggttaacta tctctgccgg
    11341 atctgcattg gcaaactctc gtagacgagc ctcttctgcc gaaacatcat aaccagaaga
    11401 tgatgttaca ccgtcactac taccaccaac tccttggcta atgccggaag aatcaaatcg
    11461 tgcaacaggc gaaaacaacg tttctggtac tctgatatcc gtaaccggtg taaccgggtt
    11521 gaccgtagac tggccccctg aagtttgagt attttttttg tctgaaacag gtccgttact
    11581 ctcctgcttt cctgacgact ggacggttct actgtcagat tccgttttgt ctctgtcttg
    11641 tggtaaatga gcctccataa acgctcccat tggatcatca attaccccta tagagcgagt
    11701 tacgacctcc gctttaatcc ctgtttcgtc tatttctggt tctggatctg cacgattatt
    11761 aataactatt gcggcaaata taattgtaaa caaaagaaca ataacaatac cgccaataat
    11821 tacaccaact attttccagt taacaggctt cttacgacca gctggtttta aagaaaatgc
    11881 gcccacacct tgaccttgag gcttattttc atcagcagcg ctagtttcct ttttcgtttc
    11941 attctctggt ggtgtcacta cattttcagt cattatttga cctccctgac ttcacctgta
    12001 gttgttccct tataattgta ccatcctgta ttttctacgt tagaacgtac agcaagaacc
    12061 agtctgttaa gacgaattct gaactcacga ctaacaccat gcacaaccaa aatatcgttt
    12121 acaggacgcg cattaaccat agtttctgta ccatcaggta gaacttcata aataactgga
    12181 cgtggcgcat ttgtcggaaa acgaaaacag gtaaatgtac cattatccca tacctgataa
    12241 ggcgaaataa tcatatcacc acgtttttgg tattttttat taataatacc atcacacggg
    12301 ttttcgttta tattcctccc aacaaatgga tttttaccaa cttctggcgg ttttggataa
    12361 ttgaacctga acagatatgt tgctaaagcc ggtgattgtg tcagatgtaa ttcaaataaa
    12421 tatgtccggc gattcgtact tacaattaag tttgttctgg gatcctccgc atatcgactt
    12481 ggttttatca ttattaaact tccggcctta ttagcaccga gtaaccattc aggtgccccc
    12541 tctgttcctg gtttccccag attcattgca cccgcatcag tatttattgt ctccccttgt
    12601 tccagttgta tcattgttgt tctgtccttc atggcgtaaa ttctgaaaac gttatcagga
    12661 ctatacaatg ccgtctggat ccttttgtcg ctgactgaac cttttccggc tatttccgca
    12721 tgaacaaaac cagtaactga aaatataaga catacaggaa ctatatattt aattattttc
    12781 ctcattcctg atctcctctg tttttgtata tgcaagcaca ccaaagccct taggatttaa
    12841 ccaggcttca ccttccgtat cagccgtatt taaataatca aaagatatta ttgctgtcca
    12901 gaaaaccact ttgggcttaa ctattggttt cccgttttca tccaacttca gtatctgtcc
    12961 tgtctctgga acaggccgcc cttctgcatc aagcagtgat tgtctgaaac gaacctgata
    13021 agttttgatg tcatccctct cacctaattt tttatcagta ctgacaggta aaggaactat
    13081 tgaatcaata tcaacagaaa tctgacgaga ctgatttaat gttgagacgt accctttacc
    13141 tgacaactga aaattacgat attcagaaaa cacatcggaa taagacaact gccttactat
    13201 tgcaaaatta gatttttgac tagcccagtt atacgtttct ctagataaaa tatataatga
    13261 tataaaatgc ttatcatcag aaatatcttt tgtatcttcc ttactccatc cgggacgaac
    13321 aacatccata tacccactat tgcgatctac tcggataaca aaaggctcta ctgttttaag
    13381 tggagctaaa tttgcaactg caattacagc aacaatagct ataacaccaa acaatccagc
    13441 caaaatacga ctatttctag ctttctttct gtaatcttca gccacccttt tttcaaacca
    13501 acgggcagcc attgtgtaag cccttatttc cctcccaatt tcatcactct tacctgataa
    13561 tttgccgtca ccataatgca agagacgttc ctgttcagtt tcctttttta tcttattact
    13621 tttaaataac atttttttct cccggtaaca gcacagactc tggatttaca tcaatccatt
    13681 caccagatgg ttgggacaac tctggcggtg aactacatga tatgcatccc agcacaacac
    13741 aaagcgccgc acatatagcg aatatatttt tcatgataag cctttttata aatgaagttt
    13801 ttgtaaagaa gacgagaaaa tggattataa gcagagatag aaaaattact aggaaatacg
    13861 acctttccct ctaccatgaa aacgatcaac taatttactt cctaatgcct tactcatgtg
    13921 acttgcagca ccaaaaccag ttgcttttcc taaaagcctg gacatactcc ctaccccacc
    13981 agatggagcg cctcccataa tagatgcagc ataatttgga atttctgtag ccagtaaagt
    14041 aaatgcacta aaatataaag acatacatat aatatcaacc attgatatat taaagtcccc
    14101 attattaatc atattgctat taataaatga aatagccatt ccaagagtaa tagcaaaaag
    14161 ggcctgtgta agaacaaatc ctcctaaaac tcccacccat ccccaaaacc aatgagctac
    14221 tttaggaaaa gctaaacatg caataaacat tggaccaatg ataagaatta gatttgttgc
    14281 gactttaagc gtcagcaatg ttgctatagc cacccctaaa aaaggcaatc cacttagtaa
    14341 atataaaaca acagcaacac cgacagtaac catacttttt attgatgata ca
//
LOCUS       DAZGAP010000139        14318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGAP010000139 DAZGAP010000000
VERSION     DAZGAP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.140"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..416
                     /locus_tag="ACHLAM_003710"
     CDS             60..416
                     /locus_tag="ACHLAM_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7855172.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(467..1363)
                     /gene="yhaJ"
                     /locus_tag="ACHLAM_003711"
     CDS             complement(467..1363)
                     /gene="yhaJ"
                     /locus_tag="ACHLAM_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO7855173.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1468..2169
                     /locus_tag="ACHLAM_003712"
     CDS             1468..2169
                     /locus_tag="ACHLAM_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO7855174.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2192..2356
                     /gene="yhaL"
                     /locus_tag="ACHLAM_003713"
     CDS             2192..2356
                     /gene="yhaL"
                     /locus_tag="ACHLAM_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO7855175.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2488..3798)
                     /gene="cyuA"
                     /locus_tag="ACHLAM_003714"
     CDS             complement(2488..3798)
                     /gene="cyuA"
                     /locus_tag="ACHLAM_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO7855176.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYEIISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3826..5157)
                     /locus_tag="ACHLAM_003715"
     CDS             complement(3826..5157)
                     /locus_tag="ACHLAM_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7855177.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5432..6796)
                     /gene="tdcG"
                     /locus_tag="ACHLAM_003716"
     CDS             complement(5432..6796)
                     /gene="tdcG"
                     /locus_tag="ACHLAM_003716"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7855178.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6868..7257)
                     /locus_tag="ACHLAM_003717"
     CDS             complement(6868..7257)
                     /locus_tag="ACHLAM_003717"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO7855179.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7271..9565)
                     /gene="tdcE"
                     /locus_tag="ACHLAM_003718"
     CDS             complement(7271..9565)
                     /gene="tdcE"
                     /locus_tag="ACHLAM_003718"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO7855180.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9599..10807)
                     /gene="tdcD"
                     /locus_tag="ACHLAM_003719"
     CDS             complement(9599..10807)
                     /gene="tdcD"
                     /locus_tag="ACHLAM_003719"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO7855181.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10833..12164)
                     /gene="tdcC"
                     /locus_tag="ACHLAM_003720"
     CDS             complement(10833..12164)
                     /gene="tdcC"
                     /locus_tag="ACHLAM_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO7855182.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12186..13175)
                     /gene="tdcB"
                     /locus_tag="ACHLAM_003721"
     CDS             complement(12186..13175)
                     /gene="tdcB"
                     /locus_tag="ACHLAM_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO7855183.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13274..14212)
                     /gene="tdcA"
                     /locus_tag="ACHLAM_003722"
     CDS             complement(13274..14212)
                     /gene="tdcA"
                     /locus_tag="ACHLAM_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO7855184.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT         3698 a         3839 c         3345 g         3436 t
ORIGIN      
        1 tagcatttac acaatacccg gaatagactt tcgtcacttt taattaaagg gatgttttta
       61 tgcagtggta tttagccgta ttaaaaaatt atgttggttt ctctggtcgc gcacgtcgta
      121 aagagtactg gatgtttact ctgattaacg ccatcgtcgg cgctattatc aatgtcattc
      181 aattgatttt aggtttagag tttccattcc tttctctcat ttacctggca gcaacaatca
      241 ttccggtcat tgctctgtgc gttcgtcgtc tgcacgatac tgaccgttca ggtgcgtggg
      301 cgcttctcta tctggttccg atcattggct ggttagttct gtttgtattt gcctgtttag
      361 aaggcaactc tggcagcaat cgttacggaa acgatccgaa gtttggttca aattaattat
      421 agaattgggg cgatatttcg ccccttttta ttaacaataa caaatattat tttccgctaa
      481 aaagtttggg aatttcccgc agacaccagg atttcgcctc ccccatactg tcacgacgcc
      541 aggccataat aatatcgatc tcgctggtcg attccgggct gacgacacgc aaccgccctt
      601 ccgcgatgtc tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta
      661 acgcctgacg tttatcttca atcgtgctca ccgttaaacg cggctgtttg tccagtagct
      721 gtacggtcaa taccgggcgc tcacgagcgg tatccgccac cgcaattcca cgatatttca
      781 cgcgcgtcac ttcagataac ggttccggct cctgatgaat cgggtgatca ggcgcggcaa
      841 cgtagacgtt cattaacgta tagagcttgc gcgagttgat ctccgacgag gagcgaaaat
      901 gcatatccgg cgcgataaca atatccgccc gcccctgctc cagccgttcc catgcccccg
      961 ccagcacttc ggtgatgatt gccagttggg tattcgcttt tgccgccagt ttgtcaatta
     1021 acgggaaaaa ggcaggtgtc ggcaccagcg cttcggtcac aatggtgaga tgcgtttccc
     1081 aaccgcgcgc gagagcttcc gcatcggtag tcagtttatc tgcggcttcc agcaaaacgc
     1141 gtcctcgctc cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt
     1201 caaacagcac cacatccagc tcttcttcca gtttttgcat ggtgtagcta agtgcggaag
     1261 gcacgcgtcc cagctcatcc gccgccgccg caaaactgcc ccggcgatcg atcgcatcca
     1321 taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga
     1381 aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata
     1441 aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga
     1501 caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac
     1561 ccgaaattgt taggctatgc ctccctgcgc gtgcttaacc aggaagtgct ggccccaggt
     1621 gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga
     1681 gaagcagagt atcgcgacag cgaaggcaat catgttcagg ccagcgctgg tgaggcgttg
     1741 ctgctctcta cccagccggg tgtcagctat agcgaacaca atctcagcaa agacaaaccg
     1801 ttaacgcgaa tgcagctttg gctggatgcc tgcccgcagc gagagaatcc gctgattcaa
     1861 aaactggcgc ttaatatggg caagcagcaa ttaatcgcct cgccagaggg cacagtgggt
     1921 agcctgcaac tgcgccagca agtgtggctg caccatatcg tgctcgacaa aggcgaaagt
     1981 gcgaatttcc agttgcatgg accacgcgcg tatttgcaat cgattcacgg gaaatttcat
     2041 gcgcttacgc atcatgaaga gaaagcggcg ctaacctgcg gtgatggggc gtttattcgt
     2101 gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg
     2161 cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcggcca aaaagcgcca
     2221 gccagtgaag cccgtggtgg cgaaagaacc tgctcgtacc gccaaaaatt ttggctatga
     2281 agagatgttg agcgaactgg aagccatcgt cgcggatgct gaaacgcgtt tagccgagga
     2341 tgaagctacc gcgtaaagac aggtgccgga tgcggcgtaa acgccttatc cggcctacaa
     2401 aacccaacaa catcaacaaa ttgtgcaatt cgtaggccag ataagacgcg ccagcatcgc
     2461 atccggcacg atacccaaaa ctggcgttta tctggccttg ctcgccataa tctcgataat
     2521 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     2581 ctcaacatca tgcgccacga tcccctcatt gccggtcacg gcggtatcat ccagcgccat
     2641 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     2701 cgctccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgaaat
     2761 gatttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     2821 cgttgcggca cacagcgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     2881 atgcgaaagc atcagcgcac gcgccagccg ttcatcatcg gccccgaagt gttctgctac
     2941 caccaccacg ggcattgttg cagtgatccc ctggttaccc gagccggagt tactcattgc
     3001 cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
     3061 tgaagagaga tctttcgcca gcaaaccgcg tgcacactgt ttttccagcg tcgcgccaat
     3121 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
     3181 atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc
     3241 cagcgtcgtt cttgaaagca ccgtcagcgg cgactcttgc tcgccctctg tcacacacgc
     3301 ctgctgggta aacaccacac cattgtgcgt ctcaatatgc acaatgttgg tatgcccgcc
     3361 gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
     3421 ttcatcgcaa ggttcctgga tcttaactga gactttcccc gccgccagca gtgctttggc
     3481 atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tagcatttcc
     3541 acctaacgct cccagcgccg ccgcaattgg cagccccacc attcccgtgc cgggaacggt
     3601 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
     3661 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
     3721 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
     3781 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
     3841 gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt
     3901 gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat
     3961 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc
     4021 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacgagat tttcgttaat
     4081 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
     4141 aaagcctaaa tagacgccaa agaatgcagt cattacagca aagatattga gaatgacgct
     4201 gaccactttc acccacgctg cgccatcacc gctaataaac tgtgcggcaa tcgccaacgc
     4261 ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga
     4321 cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa
     4381 tgctttatgc cgcgccactt caattgattt ttcccgcgag cgataagaga tcaccatcgg
     4441 gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt
     4501 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
     4561 catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat
     4621 tttaaataac aatttctcgc cgcgtgagga gatcgccacc agaatgcaaa tcagtaccag
     4681 accataaaag ggactgtctg acagcacccc ttccgtcacg ccgaaggtat gcaggtagga
     4741 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
     4801 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctga tgacgctcgg
     4861 gtaatcttta cactccggtg attctgccag cgtattaata aacaaccgct gaaacagata
     4921 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
     4981 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
     5041 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
     5101 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat
     5161 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
     5221 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
     5281 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
     5341 aatcgcaaga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
     5401 gtgcacccaa ggatgaaagc tgacagcaat gtcagccaca gaccacttta atggccagtc
     5461 ctccgcgtga cgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
     5521 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
     5581 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
     5641 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
     5701 atacctgcgc cggactgccg cccaggagtt cagttaaccc tgccgccgcc attgaacagg
     5761 ccacgccaat ctccccctga cagccgacct ccgcaccaga gatggaggcg ttcattttat
     5821 acagcgcgcc aatagccccc gctgcaagaa aatagcgggc aattgaccgc tcgtttaccg
     5881 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
     5941 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
     6001 tattgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgctg gaaaccagtt
     6061 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
     6121 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt
     6181 caatttccgc tttactgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
     6241 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     6301 gtgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     6361 cagagtaata ggttttgctt aatagctctt cctgtccctt ccaggcagtg atccgcattc
     6421 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     6481 caacaatatg cgcgccagaa gccaccggca accgcccgct gcgcgtcact aactctataa
     6541 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgatga
     6601 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac agatcgacca
     6661 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     6721 cggcattcat tggccccaca gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     6781 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     6841 ccttgcgacc gtcccgctca cgaggcctta cgcactacgt actgcgatgg cttcaatttc
     6901 caacttcaca tctttcggca aacgcgcgac ctgcacacag ctccgggtcg ggtaggtcgc
     6961 ctgatgttca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
     7021 ggtgatgaac acggtcatct tgatgatatc gcccacactc agcccggcag caaccacgat
     7081 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     7141 ggtctgtggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
     7201 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     7261 tcctccggcg tcagagcgcc tgggtaaagg tacgtgaaat aacatcctgt tgctgttcac
     7321 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     7381 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
     7441 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
     7501 tggttttacg tactggatct tctttgccca gcgccgcagg gacgatggag aaggtgtacg
     7561 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     7621 cgcctttgcg atcacgaccg tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     7681 ggcgaccgtc cggcgtgttc ccggttttct gaccgtacac cacgttagag gtgatagtca
     7741 gaatcgattg agtcggcaca gcattgcgat aggttggcag agctttaatt ttcttcataa
     7801 agcgttcaac cagatcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccatactgcg
     7861 gatattcgcc gtcgatttcg aaatccaccg ccaggccgtt ttcgtcacgg attggtttca
     7921 cacgagcata tttgatggca gacagggagt ccgttgccac cgacaggccc gcgatgccgc
     7981 acgccatggt gcgatagaca tcacgatcgt gtagcgccat cagcgaggct tcatagctgt
     8041 acttgtcgtg catgtagtgg atgatattca gcgcgctgat gtactgtacc gccagccagt
     8101 ccatgaagtg gtcgaggcta tccatcactt tgtcgtagtc cagcacgtca tccatcaacg
     8161 gagccgtttt cgggccaacc tgaatcttca gcttctcgtc cattccgccg ttgattgcgt
     8221 acagtagcgt tttcgccagg ttagcgcgcg caccgaagaa ctgcatctgc ttaccaatca
     8281 ccattgggct gacgcaacag gcaatcgcgt aatcgtcgct attgaagtca gtacgcatca
     8341 gatcgtcgtt ttcatactgc aaagaggatg tgacgataga cacctgcgcg gcatattttt
     8401 tgaaggcaat cggcaaggct tccgaccaca gaatagtcag gttaggttcc ggtgcaggcc
     8461 ccatagtgtg cagggtgtgt aaatagcgga aggagttttt ggtcaccagg gttcgaccgt
     8521 ccagccccat cccgccgatc acttccgtcg cccagatcgg gtcgccggag aacagcgaat
     8581 caaattccgg cgtacgcaag aagcgcacca tacggatctt catgatgaag tggtcgatca
     8641 gttcctgtgc ctgctgctca ttgagtacgc ctgctttaaa gtcgcgctca atgtagatat
     8701 cgaggaacga tgccgtgcgc cccagcgaca tcgcgccgcc attttgcgat ttcactgccg
     8761 ccaggtaagc gaagtagagc cactgcaccg cttcctgtgc attctgcgcc ggacgggaga
     8821 tgtcaaaacc gtatttcgcc gccatttctt gaatctgcaa cagcgcacga cgatgttccg
     8881 ccagctcctc acgcagacgg atcgtagctt cgagatcttc gcctttttcc agacgggact
     8941 ggagatcggc aaactgcagt tcgcgttcac gtaccagata actgatgccg taaagcgcca
     9001 cgcggcggta gtcaccgatg atacgcccac gaccataacc atctggtaaa ccggtcagta
     9061 caccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
     9121 gggttttacg cagatcggta aagatgtatt caaattcgct atccatttca cgaccatacg
     9181 cgtggaatga acttctaatc atattaatgc cgccgaacgg atgcagcgca cgctttaatg
     9241 gcgcatccgt ttgcagaccc actatttttt ccagcggctg gtcaatatat cctgcatcat
     9301 gagcggtaat tgtggtggca atattggtat cgaaatccaa tggcgcgtga gtcgcatttt
     9361 cgatacggat gccttccatc actttttccc acaattccgt ggtggcaggc gtcgcttcgg
     9421 cgagaaaaga ttcatcacct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
     9481 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
     9541 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
     9601 atgcaaattc tgcgggcgcg ttcactttgc ctaaatgaat ggcatccaaa gcaatcattt
     9661 tttcttcgtt agtcggaatt acggcgcaaa tgacacgcgc attttcactg gaaacaattc
     9721 gctctccaaa agagttagag cgattattca tttctgtatc aatctctacg cctaatacag
     9781 ctaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
     9841 atataattcc atccaggcga tgtaatgaag cagcgtgtcc ggcaatatga cgggcaattc
     9901 gatgaacaaa ggttttaatt gccaattgcg cgcgttcgtg accttcatgc caggcttttt
     9961 ccagaacacg taaatccgaa gaaaggccgg aaatacctaa taatcccgac tctttattca
    10021 cgacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgccc
    10081 cgaagtcgac atcaccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
    10141 ttgaggtatc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
    10201 gcgccacaac caggccggag tcatcttccg ccagattcag caaagaatgc gcgcgctggg
    10261 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
    10321 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
    10381 caaataccgc tacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
    10441 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
    10501 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
    10561 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
    10621 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
    10681 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
    10741 tgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
    10801 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
    10861 atagtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
    10921 agagacggcg ctttacgaat ggcatacatc ggcaacaggc atagcagtga tgcgataatc
    10981 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    11041 gtggagccca tgatgaagat catgctgaga gtattcagtt tacccagcga cactttggtt
    11101 ttgtcacctt tataaccgaa cttcagaatc aggccattca agccttccag cgtccccaga
    11161 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    11221 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
    11281 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac
    11341 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    11401 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    11461 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
    11521 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    11581 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
    11641 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    11701 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
    11761 ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    11821 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    11881 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    11941 agacacagac gcgccagtgc ccggtggcaa taaaacgcga tggggtatgc taataccaac
    12001 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    12061 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    12121 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    12181 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    12241 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    12301 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    12361 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    12421 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    12481 ccggtagttc ggtgcgtagt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    12541 ttttctgact gtacgccaat aacacgaata gtcgggttaa ttgatttaat cgctaccgca
    12601 ataccagcaa ttaaaccgcc accgccaatt ggcacaatca cattatcgac atcatagaga
    12661 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
    12721 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc aatagtgtcg
    12781 ttgaagttat caccatgcaa aacgacttct gcggagtagt cgcacgttgc cgcgactttg
    12841 gatttcggcg caccttttgg catcaccact ttaccgtcaa tacccagcat cgcacaggag
    12901 agcgaaaccc cttgcgcatg gttgcccgca gagcaggcca ctacaccttt acgtttttcc
    12961 gcatcagtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga accggtacgc
    13021 tgcatgtttt cgaatttgag gaatatttca cctttgcaac gttcactaaa atagttggag
    13081 cgaggcatac ctgttttata aattcgccca gccagtcgtt gttttgcttc aatgatgtca
    13141 tcaatagcaa ccgggagatc gtatgtaata tgcattataa aacctcttag cctgaattaa
    13201 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
    13261 ttaaaacaaa taactaatca acttcaatta attgccttcg tctacaccca ttataagatg
    13321 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    13381 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    13441 gattagaacc aaaaggtgat gtcatatcac aaggaattac agttaagaaa tcagcattga
    13501 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    13561 catttctttg taacgtagta agtagttcgc tgtagtaccc catattggtt tgtggcaaca
    13621 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    13681 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    13741 tttctgcgct taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
    13801 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    13861 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    13921 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    13981 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
    14041 ttttccgcac cactaactcc acaccaaaat aatcttcaat atcgttaatg attttactga
    14101 cggccggttg agttaaccct aactcttttg cagccgagcc gatagaacca cttctaataa
    14161 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    14221 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
    14281 gaccactcac aaattaactt tcaatacttt ccagggta
//
LOCUS       DAZGAP010000140        14195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGAP010000140 DAZGAP010000000
VERSION     DAZGAP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.141"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..878)
                     /gene="dinD"
                     /locus_tag="ACHLAM_003723"
     CDS             complement(54..878)
                     /gene="dinD"
                     /locus_tag="ACHLAM_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO7855185.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVNIKQLENSVKITEKK"
     gene            complement(1100..1963)
                     /locus_tag="ACHLAM_003724"
     CDS             complement(1100..1963)
                     /locus_tag="ACHLAM_003724"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO7855186.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            2090..2806
                     /gene="rph"
                     /locus_tag="ACHLAM_003725"
     CDS             2090..2806
                     /gene="rph"
                     /locus_tag="ACHLAM_003725"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO7855187.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            2872..3513
                     /gene="pyrE"
                     /locus_tag="ACHLAM_003726"
     CDS             2872..3513
                     /gene="pyrE"
                     /locus_tag="ACHLAM_003726"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO7855188.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3550..4146)
                     /gene="slmA"
                     /locus_tag="ACHLAM_003727"
     CDS             complement(3550..4146)
                     /gene="slmA"
                     /locus_tag="ACHLAM_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO7855189.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(4253..4711)
                     /gene="dut"
                     /locus_tag="ACHLAM_003728"
     CDS             complement(4253..4711)
                     /gene="dut"
                     /locus_tag="ACHLAM_003728"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO7855190.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(4689..5909)
                     /gene="coaBC"
                     /locus_tag="ACHLAM_003729"
     CDS             complement(4689..5909)
                     /gene="coaBC"
                     /locus_tag="ACHLAM_003729"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO7855191.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            6081..6749
                     /gene="radC"
                     /locus_tag="ACHLAM_003730"
     CDS             6081..6749
                     /gene="radC"
                     /locus_tag="ACHLAM_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7855192.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            6966..7202
                     /gene="rpmB"
                     /locus_tag="ACHLAM_003731"
     CDS             6966..7202
                     /gene="rpmB"
                     /locus_tag="ACHLAM_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO7855193.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            7223..7390
                     /gene="rpmG"
                     /locus_tag="ACHLAM_003732"
     CDS             7223..7390
                     /gene="rpmG"
                     /locus_tag="ACHLAM_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO7855194.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            7488..8297
                     /gene="mutM"
                     /locus_tag="ACHLAM_003733"
     CDS             7488..8297
                     /gene="mutM"
                     /locus_tag="ACHLAM_003733"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO7855195.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(8336..8815)
                     /gene="coaD"
                     /locus_tag="ACHLAM_003734"
     CDS             complement(8336..8815)
                     /gene="coaD"
                     /locus_tag="ACHLAM_003734"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO7855196.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(8823..10100)
                     /gene="waaA"
                     /locus_tag="ACHLAM_003735"
     CDS             complement(8823..10100)
                     /gene="waaA"
                     /locus_tag="ACHLAM_003735"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO7855197.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            10513..11571
                     /gene="rfaQ"
                     /locus_tag="ACHLAM_003736"
     CDS             10513..11571
                     /gene="rfaQ"
                     /locus_tag="ACHLAM_003736"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO7855198.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            11568..12692
                     /locus_tag="ACHLAM_003737"
     CDS             11568..12692
                     /locus_tag="ACHLAM_003737"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7855199.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            12685..13482
                     /gene="rfaP"
                     /locus_tag="ACHLAM_003738"
     CDS             12685..13482
                     /gene="rfaP"
                     /locus_tag="ACHLAM_003738"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO7855200.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            13498..>14195
                     /locus_tag="ACHLAM_003739"
     CDS             13498..>14195
                     /locus_tag="ACHLAM_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7855201.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKV"
BASE COUNT         3498 a         3424 c         3678 g         3595 t
ORIGIN      
        1 ctttggctta cgcatcgggt atctcaatca acaaaaagag tttttagttt ctgctatttc
       61 ttctctgtaa tttttacact gttttccaac tgcttgatgt tgacctgcgg ggtcggcaac
      121 tcctcaggca tggttccgcc aagttcctga atggtttgcc tcaccttgcg acccacgtca
      181 aagtgtgtgg tgttagcttg ctgttttgaa ttcacgccat cccgcttgag tttttcttct
      241 gtttgggtag ctcgaaagag attagccgcc agttctgttg aacccatatg atccaggatc
      301 ttctgactct ttttcagccc cttccgctga tggatggctt tctgatctaa tccaccatac
      361 agcccccggt aaccatgatt ctgaaatatg gcgaaatcga tagctgtcgc taccccagct
      421 tgctgcgctg cctcaactaa ttgtttgtta tgttctttca actcatttct cagaaaaaga
      481 cgcttttcgt cttcacgaag ttgcctgaaa gcttcatcgt cagccagctc ctgccgtcgg
      541 gtctggatag caaaataagt ttgccctgcc gcaataaccg gtttcgcagg gtcgccgttt
      601 tgtactacca ggtaacaggc atagcgggag agtacgatgt cttttaatgc tcttttggca
      661 tttgagccaa ttttgaccat atcgaggatc tcctcgaaat ggtcatcaat tgtgtgacca
      721 ctattcagac aagcttcttt ggcgcgcgtt aatacaggta taaagtgacg atactctgaa
      781 taacccagta gttttccaag ttgccttgct gaccattgtt ctgctccgtt tgcattaatc
      841 agctttatct cttcaaaagg ttgatgatgt tcgttcattg cccactccat gtgatatcga
      901 ttgcaaggaa agtaagtcta caactgtgtt tatatacagt tgtaagtatc tcacgttctc
      961 actgcgctgg gcaaaaaaac atcgcaaaat ctggagcagg cacgcaaaac agaattgaat
     1021 gttgcgggga ggaaaaatgc cgctctgttt aagtgggcgg catttgtgaa ggctgaaaag
     1081 gaagaattgg cgttacgagt tattcaatgt tctggatctg ctcgcgcatc tgctcaatca
     1141 acactttcag ctcgatggca gaattcgtca cttcggcatt gatagatttc gacgcaagag
     1201 tgttcgactc gcggttgaac tcctgcatca taaagtccag acgacgacca accgcttctt
     1261 ttttcttcag aatgttgtag gtctctttga catgcgcttc gaggcggtcc agttcttcgg
     1321 caacgtcaat tcgttgtgcc agcagaacca actcctgctc cagacggttg ttttccagtt
     1381 gcacctgagc atcttccagc ttcgcgacca gacgctcacg ctgccattgc aggatttccg
     1441 gcatatgggc gcggactttg accacttcgg cggtgacgcc ttccagacgc tgctcgatca
     1501 atgctttcag cgcctgacct tcggtttcgc gcgcgacgat aaagtcgtcc agcgtaccat
     1561 ccaacgccgc gagaatttca gcggcaatcg cgtcgagatc ctgctcctgg gctgccatca
     1621 cgcccggcca gcgtagaata tcaaccgggt tgatttcccc ttcgtcactc tgcattttta
     1681 cccagttcgc ggcagttacc agctgtttag ccagtttttc gttgaggatc agctcacctt
     1741 gcgcgctaac atctggctca tagcgcaggg tacattccac tttaccgcgc gtcaggcgag
     1801 aacgaatacg ctcacgaacg acaggttcaa ggctacggaa ctgctccggc agacgaaagt
     1861 aagtttccag ataacgctgg tttaccgagc gcatttccca ggttgcgctc ccccattcac
     1921 ccttgatttc acgccgggcg taggcggtca tactgcggat catagacgtt cctgtttata
     1981 aaaggagagg tggaaggatt atagccatcg atgccttgtc aggataggaa taaccgccgg
     2041 aagtccgtat aatgcgcagc cacatttgtt tcaagccgga gatttcaata tgcgtccagc
     2101 aggccgtagc aataatcagg tgcgtcccgt taccctgact cgtaactata caaaacatgc
     2161 agaaggctcg gtgctggtcg aatttggcga taccaaagta ttgtgtaccg cctctattga
     2221 agaaggcgtg ccgcgcttcc tgaaaggtca gggccagggc tggatcaccg cagagtacgg
     2281 catgctgcca cgttctaccc acacccgtaa cgcgcgtgaa gcggcgaaag gtaagcaggg
     2341 tggacgcacc atggaaatcc agcgtctgat cgcccgtgct cttcgtgcgg cagtagattt
     2401 gaaagcgctg ggtgagttca ccattacgct ggactgcgac gtgcttcagg ctgatggtgg
     2461 cactcgtacc gcgtcgatta cgggtgcctg cgtggcgctg gcagatgcgc tacagaagct
     2521 ggtggaaaac ggaaagctga aaaccaaccc gatgaaaggg atggtagccg cagtttctgt
     2581 cggaattgtg aacggcgaag cggtttgcga tctggaatac gttgaagact cagccgcaga
     2641 gaccgacatg aacgtagtga tgaccgaaga cgggcgcatc attgaagtgc aggggacggc
     2701 agaaggcgaa ccgttcaccc acgaagagtt actcaccttg ttggcgctgg cccgaggggg
     2761 aatcgaatcc attgtagcga cgcagaaggc ggcgctggca aactgatttt taaggcgact
     2821 gatgagtcgc cttttttttg tctgtagaaa agtaagatga ggagcgaagg catgaaacca
     2881 tatcagcgcc agtttattga atttgcgctt agcaagcagg tgttaaagtt tggcgagttt
     2941 acgctgaaat ccgggcgcaa aagcccctat ttcttcaacg ccgggctgtt taataccggg
     3001 cgcgatctgg cactgttagg ccgtttttac gctgaagcat tggtggattc cggcattgag
     3061 ttcgatctgc tgtttggccc tgcttacaaa gggatcccga ttgccaccac aactgccgtg
     3121 gcgctggcgg agcatcatga cctggacctg ccgtactgct ttaaccgcaa agaagcaaaa
     3181 gaccatggtg aaggtggcaa tctggtcggt agcgcgttac aaggacgcgt aatgctggtg
     3241 gatgatgtga tcaccgccgg aacggcgatt cgcgagtcga tggaaattat tcaggccaat
     3301 ggcgcgacgc tggctggcgt attgatttcg cttgatcgtc aggaacgcgg gcgcggcgag
     3361 atttcggcga ttcaggaagt tgagcgtgat tacaactgca aggtgatctc tatcatcacc
     3421 ctgaaagacc tgattgctta cctggaagag aagccagaaa tggcggaaca tctggcggcg
     3481 gttaaggcct atcgcgaaga gtttggcgtt taaagaaact cgccggatga aaagtcatcc
     3541 ggcgtcatat tactgcaact gtgcggcaat tagcggccag cgggcgtcaa aatcatccgt
     3601 cgggcggtat ttaaactcgc tgcggacaaa gcgtgacagc ataccttcac agaaggccag
     3661 caactggctt gccagcaggg tttcatcggt ggtgtaacct tcaccctcac gcattctctt
     3721 ttcacgcaat acctgacgca actgcgcttc aatacgctcg aacagctggt tgatgcgccc
     3781 ttgcagacga tcctgttcga acattagcgc atgaccagtg aggatgcgag tcaggccagg
     3841 attacgctca ccaaaaccga gaagcagcaa cacaatcaga cgcaggcgcg ctgtggtgtc
     3901 cttctcatct ttcagaatca gattgatgcg agtaatcagg ctatcttcga taaactcaat
     3961 caggctatcg aacatgcggg tcttactggg gaagtggcga tacagtgccg cttcggaaac
     4021 gccgacagag gcggccagtt ttgccgtcgt gatacgttgg cttccatcgc tggattccag
     4081 catcagcgcc agagactgaa gtatttcctc gcgacggttc cttttcgcag tttgtttttc
     4141 tgccatgtta caaaataccc ctgaaaataa gcacttgcca ggcggcaccc acgctatgac
     4201 cgcaaacgaa atgtttgcgg ctatgttatg acgttattcg gatgcgtatg tgttactgac
     4261 gaccagagtg accaaagccg ccttcaccgc ggtcggtggc gtcgaaatct tccaccagat
     4321 taaattcagc ctgtactacc ggaacaaaaa tcatctgggc gatgcgttcg ccaggttgaa
     4381 tggtgaagct gtcctgacca cggttccaca cggaaatcat caactggccc tgatagtcag
     4441 aatcgatcaa tcctaccagg ttaccaagca cgataccgtg cttatgtccc aatccggagc
     4501 gcggtagcat cattgccgcc agtgaaggat cggcaatatg aatcgccagc ccggtcggaa
     4561 ccagcgtagt gtcacccgga gccagttcta cggcgtcgtc gagacaggca cgcaggtcaa
     4621 gtccggcaga gccagaggtg gcataagtcg ggagcggaaa ttccttccca acgcgcgggt
     4681 ccagaatctt aacgtcgatt tttttcatca taacgggtca cgatctcgtc gagtaataat
     4741 tggccaagga gctctttgcg ctcaagcggt aagactttat ctccgtcctg ccagaaaagg
     4801 tgtaatgcgt tgttgtcgct gttaaatcct tgagttggct gggaaacatc gttcgcgcag
     4861 atcagatcaa ggtttttacg gatacgtttt tgccgggcgt attcttccac attatttgtt
     4921 tcggcggcaa atccaacgac gtaggggcga tggtctttta gtgcggcaac gcctgcgacg
     4981 atatcggggt ttttaaccat ttttattgtt aattcatcac cctgcgtggc ctgctttttg
     5041 attttttctg gggccacggt agctgcgcga taatccgcca cggcggcgca accgataaaa
     5101 atattttgct gctgtacaga tgcattcacg gcggcttcca tttccagcgc ggtcatcaca
     5161 tcaacacgtt taacaaacgg tggcgtcggt agtgaaaccg gacctgatac cagcgtgacg
     5221 ttcgcgccac gacgggcagc ggcggcggcg atagcaaaac ccatcttgcc tgagctgtga
     5281 ttagagatat aacgcaccgg atcgagtggt tcacgcgtcg ggccggcggt aatcataatg
     5341 ttcagatgtt tcaggtcgtt gacgggcgaa aaatgcgcta ccgccatatc aacaatggtt
     5401 aacggatcga gcattcgccc agggccgata tcaccacaag cctgactgcc actgtctggc
     5461 ccccaaatga gcaaaccacg ggaagcaagc acctctaaat tatgctgcgt ggcagcggca
     5521 cggtacatct gctggttcat ggcggggagc acggctacag gcgcaggtgt ggccagacaa
     5581 atcgtcgata ccagatcatt cgccattccg gcagcaacac gggcaatcaa atctgccgtg
     5641 gcaggggcga gaatcactaa atcagcccat ttacccagct caatatggcc catagcggct
     5701 tcggctgctg ggtccagcag actgtcggaa acgggataac cagaaaccgc ctgcaagcta
     5761 agtggggtga taaaggcttt tgccgcttcg gtcatggcta cgcggacgtc ggccccgcgc
     5821 tcgcgcaaac gacgcaccag ttcaggggtt ttataggcag caataccgcc gctgacgccg
     5881 agaacgattt ttttaccggc caggctcatg atgatttttc ctgttgggtg acaccagaag
     5941 ttggcgattt tatcacaata cttttgttgt cgtgccttca cctgagaatc actttgcgag
     6001 gcgctttcca ggattgaaaa ctggccgtcg atttaacgga acggctatga caggatgcga
     6061 gcaccacaaa ggaggtgaag gtgaaaaaca attcacagct gttgatgccg cgcgaaaaaa
     6121 tgctgaagtt tggtattagc gccttaacgg atgtcgagct gctggcgcta tttctgcgta
     6181 ccggaacgcg cggtaaagat gtattaaccc tggcaaaaga gatgctggag aatttcggct
     6241 ctctttatgg cttgttaacc tctgaatatg aacaatttag tggtgttcat ggaattggcg
     6301 tggcgaaatt tgcccagtta aaggggattg ccgaactggc gcggcgttac tacaacgtgc
     6361 ggatgcgtga agaaagccct ttactcagcc cggagatgac gcgggaattt ttacaaagcc
     6421 agctcacggg tgaggagcgg gagatcttta tggtgatctt tctcgactca caacacaggg
     6481 ttataacgca tagccgtctt ttttccggca cgctaaacca tgttgaagtc catcctcggg
     6541 aaattatccg cgaagcgata aaaataaatg cctcggcgct gatccttgca cataatcacc
     6601 cttcgggttg tgctgaaccc agtaaagcgg ataaactcat tactgaacgg ataataaaga
     6661 gttgtcagtt catggattta cgcgtgctcg accatatcgt gattgggcgt ggagagtatg
     6721 tttcttttgc cgaacgcggc tggatttaac ccgctatgcg cgatccttcg ggatctttgt
     6781 ctgttcggga cttgagcaca tcgctgagtc agcgtatact acgccacctt tgagaatctc
     6841 gggtttggca tttgggcctg gcaatcgaga gttcacatag aactgcgatg accgggctgt
     6901 aaagcctgac gaggcgccaa taccccatac gaagctcgag ctaatttgat ttttggagaa
     6961 tagacatgtc ccgagtctgc caagttactg gcaagcgtcc ggtgaccggt aacaaccgtt
     7021 cccacgcact gaacgcgact aaacgccgtt tcctgccgaa cctgcactct caccgtttct
     7081 gggttgagag cgagaagcgt tttgtcaccc tgcgcgtatc tgctaaaggt atgcgtgtaa
     7141 tcgataaaaa aggcatcgat acagttctgg ctgaactgcg tgcccgtggc gaaaagtact
     7201 aagtacttag aggaaataaa tcatggctaa aggtattcgt gagaaaatca agctggtttc
     7261 ttctgctggt actggtcact tctataccac tacgaagaac aaacgtacta agccggaaaa
     7321 actggaactg aaaaaattcg atccagttgt tcgccagcac gtgatctaca aagaagcgaa
     7381 aatcaaataa tttttgcttt gatgtaacga aaaacctcgc cccggcgggg ttttttgtta
     7441 tctgcctgcc cccatattga ctgcatctgt tcattcctgg agatgctatg cctgaattac
     7501 ccgaagttga aaccagccgt cgcggcatag aaccgcatct cgttggtgca accattcttc
     7561 atgcggtggt gcgcaacgga cgcttgcgct ggccggtttc agaagagatc taccgtttaa
     7621 gcgaccaacc agtgcttagc gtgcagcggc gggctaaata tctgctgctg gagctgcctg
     7681 agggctggat tatcattcat ttggggatgt ctggcagcct gcgcatcctt ccagaagaac
     7741 ttccccctga aaagcatgac catgttgatt tggtgatgag caacggtaaa gtgctgcgct
     7801 acaccgatcc gcgccgcttt ggtgcctggc tgtggaccaa agagctggaa gggcacaacg
     7861 tgctggccca tcttggaccg gagccgctta gcgacgattt caacggtgag tacctgcatc
     7921 agaagtgcga gaagaaaaaa acggcgatta aaccatggct gatggataac aagctggtgg
     7981 taggcgtagg gaatatctat gccagcgaat ctctgtttgc ggcggggatc catccggatc
     8041 ggctggcgtc atcactgtcg ctggcagagt gtgaattgtt agctcgggtg attaaagcgg
     8101 tgttgctgcg ttcgattgag cagggcggta caacgctgaa agattttctg caaagtgatg
     8161 gtaaaccggg ctatttcgct caggaattgc aggtttacgg gcgaaaaggt gagtcgtgtc
     8221 gggtgtgtgg tacgccgatt gtggcgacta aacatgcgca gcgggcaacg ttttattgtc
     8281 ggcagtgcca gaagtaattg atgcgcgccg gatggcatac catccggcat aaactctacg
     8341 ctaacttcgc catcagcgcc tgatggacat tctccggcag gaaatgggtg acatcgccct
     8401 gatggcgcgc cacctctttc accaacgatg aagagataaa cgaccactct ttcgacggca
     8461 tcagaaacac actttccagt tccggcatta agtggcgatt catatgcgcc agctgcattt
     8521 catattcaaa atccgccacc gcacgcaggc cacgaatcag caccgtagcg tgttgattac
     8581 gggcgaagtt cgccatcaaa tcactaaacc cgaccacttc cacgttcccc agatgcgcgg
     8641 ttgcctgctg tgccagcgcc acacgctctt ccagagtaaa catcggtttt ttactgggac
     8701 tggcggcgat cgccagaata acgtgatcga acatttgcgt ggcgcgcgtc acgatatcga
     8761 tatgaccatt ggtaatggga tcgaaagtac ccggataaat cgcccgtttt tgcataacaa
     8821 cctcaatgcg ttttcggtgg caggtaaggt tccagcagtt gaagcagacg ctgtagcgcg
     8881 ccctggtttt gatacagtac ttcaacggca tgacggccat agaaactacg gtaatcggcg
     8941 tcggtgagta aagaggaaac ctcttttgca agcgacgtgg catcggtaac ggtaatcagc
     9001 ccgcttgcct gctccagccg cgcgcaaatg tctttaaagt taaaagtatg aggccccatc
     9061 aataccggaa tagcgtgtgc ggcagcttcc agcggattat gcccaccacg ttcaaccagt
     9121 gaaccgccaa caaaggcgag atcggcaatg ccatacagta acatcaactc gcccatcgta
     9181 tcgccaacca caacctgcgt gctggtggag gggacttccc ctgaagagcg tgtgatatag
     9241 cttagtccag cctggcggac aaggttaatc gcatccggga agcgttccgg atgacggggg
     9301 accaggatga gcaataaatt cgggaattgc tgtaacaatg cctgatgtgc ggcgatcacc
     9361 acactctctt cgccttcgtg agtgctggtg gcaatccata ccgggcggtg tggtgcccac
     9421 tggcggcgca gcgtcacggc tttagcagcc aactgcggcg ttacagaaat atcgaatttc
     9481 aggctaccgg taacggttac ctggttattt tttgcgccca gcgccacaaa acgtgcacca
     9541 tcttcttcat tttgcgcagc aatcagcgta atacgacgca gcaagcgacg gacgaattta
     9601 cccagtttgg cataacctgc ggccgagcgg gcagagagtc gcgcgttagc gatcaccagc
     9661 ggaattttac gtttatgtag cgccgcaatc aggttaggcc atagttcggt ttccataatc
     9721 aacactagtt tagggtcgac tttattcagg aaacgattaa gtgcatcggg caggtcatat
     9781 ggcagataaa cgtgctgaac atccttcccg aaagccgatt gtacgcgctc cgaaccggtt
     9841 ggcgtcatgg ttgtaacggt aatcggtaaa tcaggataac gatggcgcag cgcgcgcacc
     9901 aacgggatcg ccgccagagt ttcaccgacg gagacggagt gcagcatgat gccgcctggt
     9961 tttagcggat ggcggtaaaa accgtaacgt tcaccccagc gttttcgata ggccggagcc
    10021 ttacgtccgc gcacccagag ccgtatccag atcagcggct gaataaggta gagaagggcg
    10081 gtgtaaagca attcgagcat agtaaatagc tgacttatgg atgtgctggg gattctatgt
    10141 atttagctgt ggctttacca ttacttttcc cgtttttgac ttaaatagca atttacccgc
    10201 caattcttgc tgaacatcta tacttttttt gtatttttat gcgattcatt gatttctgca
    10261 gtacgtgcag gaaggaatcg cagagcaaca gtgaaagaat atggttgcta atgattttat
    10321 ttgattgatt tctttattga aaatcaacaa tgtgcttaaa aatcattcgg gtcggattgc
    10381 cgcgaaagaa gtgatacact agcgcgtcaa tatcagtgcg gtatcagtat tcaggtagct
    10441 gttgagcctg gggcggtagc gtgctttttc tgcttcacct taatcggata atctcaacaa
    10501 aaagagttac ttgtggataa gccatttcga agaattttgc tcattaagat gcgttttcat
    10561 ggggatatgt tattaactac tcccgtcatt agttcgctga aaaaaaatta ccctgacgca
    10621 aaaatcgatg tgctgcttta tcaggacacc atcccgatcc tgtctgaaaa tccagagatt
    10681 aacgcgctct acggcataaa aaataaaaaa gcaaaagcct cagaaaaaat tgccaacttt
    10741 ttccatctca tcaaggtatt acgtgccaat aagtatgacc ttatcgttaa tcttaccgat
    10801 caatggatgg ttgctatact ggtgcgctta ttaaatgccc gtgtgaaaat ttcccaggat
    10861 tatcatcatc ggcagtctgc tttttggcgt aaaagtttca cccatttggt gccgttgcag
    10921 ggtggaaatg tggtggaaag taacttatcc gtgctgacac cactgggagt tgattcgttg
    10981 gtgaagcaga caaccatgag ttacccgcct gcaagctgga aacgtatgcg tcgcgaactt
    11041 gatcacgctg gtgttggaca aaattatgtg gttatccaac ctacggcgcg gcaaatcttc
    11101 aaatgctggg acaacgccaa gttttccgct gtgattgatg ccttacatgc tcgtggttat
    11161 gaagtcgttc tgacgtccgg cccagataaa gacgatctgg cctgcgtcaa tgaaattgcg
    11221 cagggatgcc agacgccacc agtaacggcg ctggccggaa aggtgacctt cccggaactt
    11281 ggtgcgttaa tcgatcatgc gcagctgttt attggcgttg actccgcacc ggcgcatatt
    11341 gccgctgcag ttaatacgcc gctgatatcg ctgtttggtg cgacagacca tattttctgg
    11401 cgtccctggt caaataacat gattcaattc tgggcgggag attaccggga aatgccaacg
    11461 cgcgatcagc gtgaccgaaa tgagatgtat ctttcggtta ttccggcggc agatgtcatt
    11521 gctgctgtcg ataaattact gccctcctcc acgacaggta cgtcgttatg atcgttgctt
    11581 tttgtttata taaatatttt ccctttggcg gtttgcagcg cgattttatg cgtattgctc
    11641 agacagtcgc cgcccgaggt catcatgttc gggtttatac ccagtcgtgg gaaggcgaat
    11701 gccctgatgt atttgaactg atcaaagtgc cggttaaatc gcataccaat cacgggcgca
    11761 atgcggagta ttttgcctgg gtgcaaaaac atttacgcga acatcccgtc gataaagtcg
    11821 ttggattcaa caaaatgccg gggctggacg tttattatgc cgctgatgtt tgttatgccg
    11881 agaaagtagc gcaggaaaaa ggctttttct atcgcctgac gtcacgttat cgccattatg
    11941 ccgcctttga gcgggcaacc ttcgaacagg gcaagccgac acagctgctg atgctgacag
    12001 ataagcaaat cgccgatttc cagaaacatt atcagactga agcggagcgt tttcatattc
    12061 tgccaccggg gatttatcct gatcgtaaat atagccagca gccagctaat agccgtgaaa
    12121 tcttccgtaa gaagaatgga ataaccgaac aacaatattt attgttgcag gtcggttcag
    12181 acttcacgcg taaaggtgtc gatcgttcca ttgaagcact tgcttcgtta ccggattcgc
    12241 tgcgccacaa cacattgcta tatgttgttg ggcaggataa accgcgaaaa tttgaggcgc
    12301 tggcagaaaa acgcggcgtg cgcagtaatg ttcacttctt ctcggggcgc aacgatgtct
    12361 cggaattaat ggcggcggcg gatttattac tgcatcctgc ctaccaggaa gcggcgggaa
    12421 ttgtgctgct ggaagcgatt actgcaggat taccggtact aacaactgcc gtttgtggct
    12481 atgcgcatta tattgtcgac gccaattgcg gcgaggctat tgctgagcca ttccgccagg
    12541 aaacattgaa tgagatttta cgcaaagcgt taacgcaatt ttcattgcgc caggcctggg
    12601 cggaaaatgc gcgacattat gctgatacac aagatttata cagtctgcca gagaaagcgg
    12661 cggatatcat aacgggtggt ctggatggtt gaacttaaag agccgtttgc cacgttatgg
    12721 cgcggaaaag atccttttga ggaagttaaa actttgcagg gtgaggtatt tcgtgaactg
    12781 gaaactcgcc gtactctgcg ctttgaaatg gcgggcaaaa gctattttct caaatggcat
    12841 cgcggcacga ccctgaaaga gataatcaaa aatttactct cattgcggat gccagtatta
    12901 ggcgcagacc gcgaatggaa tgcgattcat cgactgcggg atgtcggcgt tgatactatg
    12961 tatggggtgg cattcggcga aaaaggcatt aatccgctca ccagaacctc gtttattata
    13021 accgaagatc tgacaccaac catcagtctg gaagattact gtgctgactg ggcgactaac
    13081 cctccagatg ttcgcgtaaa gcgtatgctt attaagcgtg tcgcgacgat ggtgcgcgat
    13141 atgcatgctg cgggcattaa ccaccgtgac tgttatatct gtcatttcct gctgcacttg
    13201 cctttttccg gtaagaaaga ggagttaaaa atttcggtaa ttgatctgca ccgggcgcag
    13261 cttcgcacgc gcgttccacg tcgttggcgc gataaagatc ttatagggct ttatttttct
    13321 tcgatgaata ttggcctgac tcagcgggat atctggcggt ttatgaaagt gtattttgtc
    13381 gcctcgctta aagacattct caagcaggaa caaggactgc tgtcgcaagc agaagcaaaa
    13441 gccacaaaaa tcagggaaag aacgattcga aaatcgttgt aatttccgga gagaaatatg
    13501 agtgcccact attttaatcc acaagagatg atcaataaga caatcatctt cgatgaaagg
    13561 ccagcggcgt cagtggcatc atcattccat gttgcttatg gcattgataa aaactttctt
    13621 tttggttgtg gtgtttcaat cacgtcagtt ttgttacata acaacgacgt aagttttgtt
    13681 ttccacgttt ttattgatga tatccctgaa gccgatatcc agcgtttagc ccaattggcg
    13741 aaaagctatc gtacctgtat ccagatccat ctagtaaatt gtgaacggct taaggcatta
    13801 ccgacgacca aaaattggtc tattgccatg tatttccgtt ttgtaattgc agattacttt
    13861 attgatcaac aagataagat cctttacctg gatgctgata tcgcctgtca gggaaactta
    13921 aagccgctga taacaatgga tcttgccaat aacgttgctg ctgttgttac tgaacgcgat
    13981 gctaactggt ggtcgttacg gggtcaaagt ctgcagtgta atgaacttga aaagggttac
    14041 tttaattcag gtgtcctgtt aattaataca ctagcgtggg cgcaggagtc cgtttctgct
    14101 aaagcgatgt cgatgcttgc tgataaagcc atcgtttccc gtttaaccta tatggatcaa
    14161 gatatcctta atcttatcct gttagggaaa gttaa
//
LOCUS       DAZGAP010000141        14194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGAP010000141 DAZGAP010000000
VERSION     DAZGAP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.142"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            206..3949
                     /gene="narG"
                     /locus_tag="ACHLAM_003740"
     CDS             206..3949
                     /gene="narG"
                     /locus_tag="ACHLAM_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO7855202.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLMGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQALDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITSQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            3946..5484
                     /gene="narH"
                     /locus_tag="ACHLAM_003741"
     CDS             3946..5484
                     /gene="narH"
                     /locus_tag="ACHLAM_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7855203.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            5481..6191
                     /gene="narJ"
                     /locus_tag="ACHLAM_003742"
     CDS             5481..6191
                     /gene="narJ"
                     /locus_tag="ACHLAM_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7855204.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            6191..6868
                     /gene="narI"
                     /locus_tag="ACHLAM_003743"
     CDS             6191..6868
                     /gene="narI"
                     /locus_tag="ACHLAM_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7855205.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7053..7184)
                     /locus_tag="ACHLAM_003744"
     ncRNA           complement(7053..7184)
                     /ncRNA_class="other"
                     /locus_tag="ACHLAM_003744"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7231..7315)
                     /locus_tag="ACHLAM_003745"
     tRNA            complement(7231..7315)
                     /locus_tag="ACHLAM_003745"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7279..7281),aa:Tyr,seq:gta)
     gene            complement(7475..8317)
                     /gene="purU"
                     /locus_tag="ACHLAM_003746"
     CDS             complement(7475..8317)
                     /gene="purU"
                     /locus_tag="ACHLAM_003746"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO7855206.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(8367..8825)
                     /gene="ychJ"
                     /locus_tag="ACHLAM_003747"
     CDS             complement(8367..8825)
                     /gene="ychJ"
                     /locus_tag="ACHLAM_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO7855207.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            8938..9843
                     /gene="rssA"
                     /locus_tag="ACHLAM_003748"
     CDS             8938..9843
                     /gene="rssA"
                     /locus_tag="ACHLAM_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO7855208.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9935..10948
                     /gene="rssB"
                     /locus_tag="ACHLAM_003749"
     CDS             9935..10948
                     /gene="rssB"
                     /locus_tag="ACHLAM_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO7855209.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            11150..12058
                     /gene="galU"
                     /locus_tag="ACHLAM_003750"
     CDS             11150..12058
                     /gene="galU"
                     /locus_tag="ACHLAM_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO7855210.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(12203..12616)
                     /gene="hns"
                     /locus_tag="ACHLAM_003751"
     CDS             complement(12203..12616)
                     /gene="hns"
                     /locus_tag="ACHLAM_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO7855211.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            13220..13837
                     /gene="tdk"
                     /locus_tag="ACHLAM_003752"
     CDS             13220..13837
                     /gene="tdk"
                     /locus_tag="ACHLAM_003752"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO7855212.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(14120..>14194)
                     /locus_tag="ACHLAM_003753"
     CDS             complement(14120..>14194)
                     /locus_tag="ACHLAM_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855213.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         3510 a         3393 c         3771 g         3520 t
ORIGIN      
        1 ggatgcaaaa tatcttaatt tacagtgatt tatgttgaat ttattaatta tccttaaggg
       61 gtatcttagg aatttacttt atttttcatc cccatcactc ttgatcgtta tcaattccca
      121 cgctgtttca gagcgttacc ttgcccttaa acattagcaa tgtcgattta tcagagagcc
      181 gacaggctcc cacaggagaa aaccgatgag taaattcctg gaccggtttc gctacttcaa
      241 acagaagggc gaaacctttg ccgatgggca tggccagctt ctcaatacca accgtgactg
      301 ggaggatgga tatcgccagc gttggcagca tgacaaaatc gtccgctcta cccatggggt
      361 aaactgcacc ggctcctgca gctggaaaat ctacgttaaa aacggtctgg tcacctggga
      421 aacccagcag actgactatc cgcgtactcg cccggatctg ccaaaccatg aacctcgcgg
      481 ctgcccgcgc ggtgccagct actcctggta tctttacagc gccaaccgtc tgaaataccc
      541 gatgatgcgc aaacgcctga tgaaaatgtg gcgtgaagcg aaggcgctgc atagcgatcc
      601 ggttgaggca tgggcttcta tcattgaaga cgccgataaa gcgaaaagct ttaagcaggc
      661 gcgtggacgc ggtggttttg ttcgttcttc ctggcaggaa gtgaacgaac tgatcgccgc
      721 atctaacgtt tacaccatca aaaactacgg cccggaccgt gtcgctggct tctcgccaat
      781 tccggcaatg tcgatggttt cttacgcgtc gggtgcgcgt tatctctcgc tgcttggcgg
      841 tacttgctta agcttctacg actggtactg cgatctgcct cctgcgtctc cacaaacctg
      901 gggcgagcaa actgacgtac cggaatctgc tgactggtac aactccagct acatcatcgc
      961 ctgggggtca aacgtgccgc agacgcgtac tccggatgct cacttcttta ctgaagtgcg
     1021 ttacaaaggc accaaaaccg ttgccgtcac gccggactac gctgaaatcg ccaaactgtg
     1081 cgatctgtgg ctggcaccga agcagggcac cgacgcagca atggcgctgg cgatgggtca
     1141 cgtgatgctg cgtgaattcc acctcgacaa cccaagccag tatttcaccg actatgtgcg
     1201 tcgttatacc gacatgccaa tgctggtgat gctggaagag cgcgacggtt actacgctgc
     1261 gggtcgtatg ctgcgcgctg ctgatctggt ggatgcgctg ggccaggaaa acaatccgga
     1321 atggaaaact gtcgccttta ataccaatgg agaaatggtt gcgccgaacg gttctattgg
     1381 cttccgctgg ggcgagaagg gcaaatggaa tcttgagcag cgcgacggca aaactggcga
     1441 agaaaccgag ctgcaactga gcctgatggg tagccaggat gagatcgctg aagtgggctt
     1501 cccatacttt ggtggcgacg gcacggaaca cttcaacaaa gtggaactgg aaaacgtgct
     1561 gctgcacaag ctgccggtga aacgcctgca actggctgat ggcagcactg ctctggtgac
     1621 caccgtttat gatctgacgt tggcaaacta cggtctggaa cgtggcctga acgacgttaa
     1681 ctgcgcaacc agctatgacg acgtgaaagc ctacacccct gcatgggccg agcagatcac
     1741 tggcgtttct cgcagccaga ttatccgcat tgcccgtgaa tttgccgata acgctgacaa
     1801 gacgcacggt cgttcgatga ttatcgtcgg tgcggggctg aaccactggt atcacctcga
     1861 tatgaactat cgtggtctga tcaacatgct gattttctgc ggctgtgtag gtcagagcgg
     1921 tggtggctgg gcgcactatg taggtcagga aaaactgcgt ccgcaaaccg gctggcagcc
     1981 gctggcgttt gctcttgact ggcagcgtcc ggcgcgtcac atgaacagca cctcttattt
     2041 ctataaccac tccagccagt ggcgttatga aaccgtgacc gcagaagagt tgttgtcgcc
     2101 gatggcggat aaatcccgtt ataccggaca tctgatcgac tttaacgtcc gtgcagaacg
     2161 tatgggctgg ctgccgtctg cgccgcagtt aggcactaac ccgctgacta tcgctcgcga
     2221 agcagaaaaa gccgggatga atccggtgga ctatacggtg aaatccctga aagagggttc
     2281 catccgtttt gcggcagaac aaccagaaaa cggtaaaaac cacccgcgta acctgttcat
     2341 ctggcgttct aacctgctcg gttcttccgg taaaggtcat gagtttatgc tcaagtacct
     2401 gctggggacg gagcacggta tccagggtaa agacctgggt cagcagggcg gcgtgaagcc
     2461 ggaagaagtg gactggcagg acaacggtct ggaaggcaag ttggatctgg tggtgacgct
     2521 ggacttccgt ctgtcgagca cctgtctcta ttccgacatc attttgccga cggcgacctg
     2581 gtacgaaaaa gacgacatga atacttcgga tatgcatccg tttattcacc cgctgtctgc
     2641 tgcggtcgat ccggcctggg aagcgaaaag cgactgggaa atctacaaag ccatcgcgaa
     2701 gaaattctcc gaagtgtgcg ttggtcatct gggcaaagaa accgacatcg tcacgctgcc
     2761 tatccagcac gactctgccg ctgaactggc gcaagcgctg gatgtgaaag actggaaaaa
     2821 aggcgaatgt gacctgatcc caggtaaaac cgcaccgcac attatggttg tagagcgcga
     2881 ttatccggca acttacgaac gctttacctc tatcggcccg ctgatggaga aaatcggtaa
     2941 cggcggtaaa gggattgcct ggaacaccca gagcgagatg gatctgctgc gtaagctcaa
     3001 ctacaccaaa gcggaaggtc cggcgaaagg ccagccgatg ctcaacaccg caattgatgc
     3061 agcagagatg atcctgacgc tggcaccaga aaccaacggc caggtggccg tgaaagcctg
     3121 ggcggcattg agtgagttta ccggtcgtga ccatacgcat ctggcgctga ataaagaaga
     3181 cgagaagatc cgcttccgtg acattcaggc gcagccgcgc aaaatcatct ccagcccgac
     3241 ctggtctggc cttgaagatg aacatgtttc ttacaacgcc ggttacacca acgttcacga
     3301 gctgatcccg tggcgtacgc tctctggccg tcagcagctg tatcaggatc atcagtggat
     3361 gcgtgatttt ggtgaaagcc tgctggttta tcgtccgccg atcgacaccc gttcggtgaa
     3421 agaagtgatg ggccagaaat ctaacggtaa tccggaaaaa gcgctcaact tcctgacacc
     3481 gcaccagaag tggggtatcc actccaccta cagcgacaac ctgctgatgc tgactttagg
     3541 tcgcggtggt ccggtggtct ggttgagtga agccgatgcc aaagagctgg gtatcgccga
     3601 caacgactgg attgaagtct tcaacagcaa cggtgcattg actgcccgtg cggttgtcag
     3661 ccagcgtgtt ccggcaggga tgaccatgat gtaccacgcg caggaacgta tcgttaacct
     3721 gccgggttca gaaatcacca gccagcgcgg tggtattcat aactcggtca cccgtatcac
     3781 gccgaaaccg acgcatatga tcggcggcta tgcccatctg gcatacggct ttaactacta
     3841 cggcactgta ggttctaacc gcgatgaatt tgttgtagtg cgtaagatga agaacattga
     3901 ctggttagat ggcgaaggca atgaccaggt acaggagagc gtaaaatgaa aattcgttca
     3961 caagtcggca tggtgctgaa tctggataag tgcatcggtt gccacacctg ttcagtgacc
     4021 tgtaaaaacg tctggaccag ccgtgaaggc gtggaatacg cgtggttcaa caacgtggaa
     4081 accaagccgg gccagggctt cccgacagac tgggaaaacc aggaaaaata caaaggcggc
     4141 tggatccgta aaatcaacgg caaactgcaa ccgcgcatgg gtaaccgcgc catgctgctg
     4201 ggtaaaattt tcgctaaccc gcatctgccg gggatcgacg attattacga gccgttcgat
     4261 tttgactatc agaacctgca taccgcgccg gaaggcagca aatcgcagcc gattgcccgt
     4321 ccacgttcgc tgattaccgg cgaacggatg gcgaaaatcg aaaaagggcc aaactgggaa
     4381 gatgacctgg gcggcgagtt tgacaaactg gcgaaagaca agaacttcga caacatccag
     4441 aaggcgatgt atagccagtt cgaaaacacc ttcatgatgt atttgccacg cctgtgcgaa
     4501 cactgcctga acccggcatg tgtggcgacc tgcccgagcg gtgcgattta caagcgtgaa
     4561 gaagatggca tcgtcctgat cgaccaggac aaatgccgtg gctggcgtat gtgcatcacc
     4621 ggatgcccgt acaaaaaaat ctacttcaac tggaagagcg gtaagtctga gaagtgcatc
     4681 ttctgctatc cgcgtattga agccggtcag ccgacggtgt gctctgaaac ctgtgtcggt
     4741 cgtatccgtt atcttggcgt gctgctgtac gatgccgacg cgattgaacg tgcagccagc
     4801 accgagaacg agaaagatct ctaccagcgt cagctggacg tgttcctcga tccgaacgat
     4861 ccgaaggtca tcgagcaggc gattaaagac ggtattccgc tgagcgttat tgaagccgca
     4921 cagcagtcgc cggtttataa aatggcgatg gaatggaaac tggcgctgcc gctgcatccg
     4981 gaatatcgca cgctgccgat ggtctggtat gtgccacctc tgtcgccgat tcagtctgcg
     5041 gcagatgcgg gtgagctggg cagcaatggc attctgccag acgtcgaaag cctgcgtatt
     5101 ccggtacagt atctggcgaa tctgctgacc gcaggtgata ccaaaccggt actgcgcgcg
     5161 ctgaaacgta tgctggcgat gcgtcattat aaacgtgctg aaaccgttga cggtaaagtt
     5221 gatacccgtg cgctggaaga ggtcggtctg accgaagccc aggcacagga gatgtaccgt
     5281 tatctggcga ttgctaacta cgaagatcgc tttgtggtgc cgagcagtca tcgtgaactg
     5341 gcgcgggaag ccttcccgga gaaaaatggc tgcggcttta cctttggtga tggctgccac
     5401 ggctcagata ccaaattcaa tctgtttaac agccgtcgta tcgatgccat cgacgtgacc
     5461 agtaaaacgg agccgcaccc atgatcgaac tcgtgattgt atcgcgtctc cttgaatatc
     5521 cggatgctgc cttatggcag catcaacaag agatgtttga ggcgattgcc gcgtcgaaaa
     5581 atctgccaaa agaggatgcc catgcgctgg gcattttcct gcgcgattta acgacgatgg
     5641 atccgctcga tgcccaggcg cagtacagcg aactgttcga ccgtggccgc gccacgtcac
     5701 tgttgctatt tgaacatgtt cacggtgaat cccgtgaccg cggccaggcg atggtggatc
     5761 tgctggcgca gtacgagcag cacggcttgc agttaaacag ccgcgaattg cccgaccatc
     5821 tgccgctgta tctggagtac ctggcgcagc tgccgcaaag cgaagccgtg gagggtttga
     5881 aagatattgc gccgattctg gcattgctga gcgcgcgtct gcaacagcgt gaaagccgtt
     5941 atgccgtgct gtttgatctg ctgctgaaac tggcgaatac cgccatcgac agcgacaaag
     6001 tggcggaaaa aattgccgat gaagcgcgcg acgatacgcc gcaggcgctg gatgcagtct
     6061 gggaagaaga gcaggttaaa ttctttgctg aaaaaggctg cggtgattca gcaatcactg
     6121 ctcatcagcg tcgctttgcc ggtgccgtcg cgccgcaata tctgaatatc accaccggag
     6181 gacagcacta atgcaattcc tgaatatgtt cttctttgat atctacccgt acatagccgg
     6241 ggcggtcttc ctgattggta gctggctgcg ttatgactac gggcagtaca cctggcgcgc
     6301 ggcgtccagc cagatgctgg atcgcaaagg gatgaacctg gcgtcgaacc tgttccatat
     6361 cggtattctg gggatttttg ttggtcactt cttcggtatg ctgacgccgc actggatgta
     6421 tgaagcctgg ctgccgattg aagtgaaaca gaaaatggcg atgtttgctg gtggtgccag
     6481 cggtgtgctg tgtctgattg gcggcgtgct gttgctgaag cgtcgtctgt tcagcccacg
     6541 cgtgcgtgca accactaccg gagcggatat cctgatcctg tcgctgctcg ttatccagtg
     6601 cgcgctgggc ctgttgacca ttccgttctc cgcccaacat atggacggta gcgagatgat
     6661 gaaactggtt ggctgggcgc agtcggtagt gaccttccac ggtggcgctt ctcaacacct
     6721 tgatggtgtg gcgtttatct tccgtctgca cctggtgttg gggatgacgt tattcctgct
     6781 gttcccgttc tcgcgtctgg tgcacatctg gagcgtaccg gtggagtatc tgacacgtaa
     6841 gtatcagctg gtgcgcgctc gtcactaagc gaattttagt ttgcatagac cccgcttcgg
     6901 cggggttttt ttatgggcac ggtacggggt gagttgtcgg atgcgcttcg cttatccgac
     6961 ctacagggga ggatattgta ggtccggtaa acgcggtgcc attgggcaga attagctcgg
     7021 agtaacaggt tttgagtatt tacaggaagt gatggtggtg gaggaaggat tactcagcgc
     7081 tgcgcgcttc gcccttcggg tcgttgcctg cggccacgct ctctcgctgg cgctcgagtc
     7141 gaaccctggt cgaagcttct catccttccc cgcttgggca gaatatttaa tcgcggattc
     7201 gtttgagagt tcacggactt ttgaaagtga tggtggtggg ggaaggattc gaaccttcga
     7261 agtcgatgac ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccacggg
     7321 gtaatgcttt ttactagcct gctcccttat cgggaagcgg ggcgcatcat atcaaatgac
     7381 gcgccgctgt aaagtgttac gttgagaaaa atgaactggt tgcgtaattt tcacccgtaa
     7441 cggattaaag gtaaccagtt atttttgctg gcgattaaag aataatcgtc cgattaccgt
     7501 aaacaaagac gcgctgcgcc agtactttgt agagtgcgcg acttaagacg tttttctcga
     7561 cgtcacgacc tgcgcgcatc atatcttcag ctgtgtaggt atgatcgaca tgaataacgt
     7621 cctgcatgat gattggacct tcgtccagat tgtcattcac atagtgagcg gtagcgccaa
     7681 taatcttcac gccacgttca taggcctggt gataaggacg tgcgccgata aacgctggca
     7741 ggaatgaatg gtgaatattg atgatcttat tcgggaagcg tgccacaaat tctggcgtta
     7801 atacccgcat atacttcgcc agcaccacgt agtcaggctg ataagcatca atggcatccg
     7861 ccatcttttg atcgtgctcg ttgcggctta acccttcatg gcttaccagc tcaaacggaa
     7921 tatcaaaacg ctcaaccaga gaacgtaaag tatcgtggtt accaataact gccgcgattt
     7981 cgacatccag gccgccgtaa ttggctttca tcaacaaatc gccaaggcaa tgcgcctctt
     8041 tagtgaccag aatcactatc cggcgacgac cggcaggatt cagctcacgt acggagcctt
     8101 ctggcaatgc gctgtcgaga tccgccagca gggtggaatc attaaaaatt ccttccagtt
     8161 ccgtgcgcat aaaaaagcgc ccggtacggt gatcaacaaa ttcattgttc tgtacgatat
     8221 ttaactcgtg cttgtagcaa atattggtaa tacgtgcgat cagacctttt tggtccggac
     8281 aaatagtacg cagaacttta cgttggagtg aatgcattgc tggaaaaacc ttgttgagag
     8341 tgtttgctaa accgtaccgt caaccattat tggccgcagc actttttaaa ttttttacct
     8401 gaaccacaag ggcagggatc gttgcggcca aactgcggac gtgtaccgtc aatatagtac
     8461 cattgaccgt tttctttcaa aaatcgcgaa cgttcaatta ttgcacctgt tttgctacct
     8521 tcggtaaatc gcgcaacaaa actgacaaat ccgatattgt cgacgtcctg ccaacaatgt
     8581 tcaaatacgg tcaatcccaa ccattcagta tgcgcaaatc cggcaatcaa ttcggcacgt
     8641 aacgtcgccg ctccacaaga tggatgccag gtctttatta aataatctgc gtctttcatc
     8701 acaaaagcgc agtaacgcga acgcatgaga tgttctggat caggtgcaac cttttcacca
     8761 gacacataag ggtggcaaca taggctatac tcgacagcac taccacaggg acaaagctga
     8821 gacacaaata atctccctgg aaacaataac ggcgtattaa ccgcctgagt agcactatgt
     8881 taaccgagca gtagcgatgt tgctacgatt gcattccagg ggaatcttgc gggaataatg
     8941 agaaagataa aaatagggct ggcgctggga tctggcgcgg cgagaggttg gtcgcatatt
     9001 ggcgttatta atgcgctaaa aaaagtgggt attgaaattg atatcgttgc aggatgttca
     9061 attggttcgc tggtgggcgc tgcctatgca tgcgatcgac tatctgcgct ggaagattgg
     9121 gtgacctctt tcagttattg ggatgtttta cgcctgatgg atctctcctg gcagcgcggt
     9181 gggttactgc gcggcgagcg tgtcttcaat caatatcgcg aaataatgcc ggaaacagag
     9241 atcgaaaatt gttcccgtcg cttcgcggct gttgccacca atttaagtac tgggcgcgaa
     9301 ttatggttta ctgaaggcga tctccatctt gctattcgtg catcatgcag tattccagga
     9361 ctaatggcac ccgttgcgca taacggctac tggctggttg atggcgctgt agttaaccca
     9421 attcctattt ccctcacgcg tgcattgggg gctgatattg tgatagcggt cgacctgcag
     9481 cacgatgctc atttgatgca acaagatttg ctctccttta atgtcagtga agaaaatagc
     9541 gagaatggtg attctctgcc gtggcatgcg cgtctgaaag aaaggttagg cagcataacg
     9601 acacgtcggg cggtgacagc gccaacggca acagagatta tgaccacttc tatccaggtg
     9661 ctggagaacc gccttaaaag gaaccgcatg gcaggtgatc cgcccgatat tctgattcaa
     9721 cctgtttgcc cgcaaatatc tacgcttgat ttccatcgcg cgcacgctgc cattgcggcc
     9781 gggcagctgg cagtggaaaa gaaaatggac gaacttttgc cgttggtacg caccaacatt
     9841 tgaccagaat ttttatctac acttaagtta attctgacag gcgcaggtgg caatagcatg
     9901 ccactattga gtaaagccag tcaggggaga gaacatgacg cagccattgg tcggaaaaca
     9961 gattctcatt gttgaagatg agcaggtatt tcgctcgctt ctggattcgt ggttttcctc
    10021 attgggagcg acaacggtac tggcggctga tggggtggat gcccttgagt tgctgggagg
    10081 tttcactcca gacctgatga tatgtgatat cgcgatgcca cgaatgaacg ggcttaaact
    10141 gctggagcat atacgtaaca gaggcgacca gaccccagtt ctggtgatat ctgccactga
    10201 aaatatggca gatattgcca aagcgttacg tctgggcgtt gaagatgttt tgctgaaacc
    10261 agttaaagat ctgaatcgct tgcgcgagat ggtttttgcc tgtctctatc ccagcatgtt
    10321 taattcccgc gttgaggaag aggaaaggct ttttcgcgac tgggatgcaa tggttgataa
    10381 ccctgccgca gcggcgaaat tattacagga attgcaaccg ccggttcagc aggtgatttc
    10441 ccattgccgg gttaattatc gtcaattggt tgccgcggac aaacccggcc tggtgcttga
    10501 tattgccgca ctttcggaaa acgatctggc attttactgc cttgatgtca cccgagctgg
    10561 acataatggc gtacttgctg ccttgttatt acgcgcattg tttaacggat tattacagga
    10621 acagcttgca caccaaaatc aacgtttgcc agagttgggc gcgttattga agcaggtaaa
    10681 ccatttactc cgtcaggcca atctgccggg gcagtttccg ctattagttg gctattatca
    10741 tcgcgaactg aaaaatctca ttctggtttc tgcgggtctg aatgcgacgt taaataccgg
    10801 cgaacaccag gtgcaaatca gtaatggtgt tccgttaggc actttaggta acgcttattt
    10861 gaatcaattg agccagcgat gcgatgcctg gcaatgccaa atatggggaa ccggtggtcg
    10921 gcttcgcttg atgttgtctg cagaatgagc aaacgataac gcgggctaaa tttgcattac
    10981 ctgctaatgt cggctggtgg tactatcgtc gccattcgta taagtaattg tcttaattat
    11041 gctaactcgc ctccttttca gaacttagcc ccttcggggt gctgatatac tgggatgcga
    11101 tacagaaata tgaacacgtt caaaacacga acagtccagg agaatttaaa tggctgccat
    11161 taatacgaaa gtcaaaaaag ccgttatccc cgttgcggga ttaggaacca ggatgttgcc
    11221 ggcgacgaaa gccatcccga aagagatgct gccacttgtc gataagccat taattcaata
    11281 cgtcgtgaat gaatgtattg cggctggcat tactgaaatt gtgctggtta ctcactcatc
    11341 taaaaactct attgaaaacc actttgatac cagttttgaa ctggaagcaa tgctggaaaa
    11401 acgtgtaaaa cgtcaactgc ttgatgaagt gcagtctatt tgtccgccgc acgtgactat
    11461 tatgcaagtt cgtcaggggc tggcgaaagg cctgggacat gcggtattgt gtgctcaccc
    11521 ggtagtgggt gatgaacctg tggctgttat tttgcctgac gttattctgg atgaatatga
    11581 atccgatttg tcacaggata acctggcaga gatgatccgc cgctttgatg aaacgggtca
    11641 tagccagatc atggttgaac cggttgctga tgttaccgca tatggcgttg tggattgcaa
    11701 aggcgttgaa ttagcgccgg gtgaaagcgt accgatggtt ggtgtggttg aaaaaccaaa
    11761 agcggatgtt gcgccgtcta atctcgctat tgtgggtcgt tacgtactta gcgcggatat
    11821 ttggccgttg ctggcaaaaa cccctccggg agctggtgat gaaattcagc tcaccgacgc
    11881 aattgatatg ctgatcgaaa aagaaacggt ggaagcctat catatgaaag ggaagagcca
    11941 tgactgtggt aataaattag gttacatgca ggccttcgtt gaatacggta ttcgtcataa
    12001 cacccttggt acggaattta aagcctggct tgaagaagag atgggcatta agaagtaaca
    12061 tccgtatcgg tgttatccac gaaacggcgt tgagcaatcg acgccgtttt tttatagctt
    12121 attcttatta aattgtctta aaccggacaa taaaaaatcc cgccgatggc gggattttta
    12181 agcaagtgca atctacaaaa gattattgct tgatcaggaa atcgtcgagg gatttacctt
    12241 gctcatccat tgcttttttg attacagctg gagtacggcc ctggccagtc caggttttag
    12301 tttcgccgtt ttcgtcaacg tagctatatt ttgccggacg ctgagcacgt ttagctttgg
    12361 tgccagattt aacggcagca aggctattca gcagttcgtt cgggtcaata ccgtcagcga
    12421 tcagcatttc gcgatattgc tgcagtttac gagtgcgctc ttcaacttca gcagcagccg
    12481 cgctttcttc ttcgcgacgt tcgttaacaa caacttctaa tttttccagc atttcttcca
    12541 gcgtttcaag tgtacattct cttgcctgcg cacgaagagt acggatgttg ttcagaattt
    12601 taagtgcttc gctcattgta gtaatctcaa acttatattg gggtggtttg ttgaggtaat
    12661 aatagagcct taaattcagt tgtgcaatag ccaggaatgt aaggaattca aaattgttct
    12721 ttattttgtg ccgccaataa atatcttttc ataaaattag ccagaaaaga cgcggcatat
    12781 agccctattt gcgccgatga tttcgcagca cgtgaggtta aaacttcctg attcatgtca
    12841 cattttatgg ggagattatc gtaggctgac gacctttcag tcttctgtat tagtcgtgtt
    12901 tacaagaatt ccctattaag cgaatgatga aaagtagaac agtcgcaata agagcaggga
    12961 cttagtattg cactatctcc tggaggtcaa cagagggcta ttacttgcgc aacaggttaa
    13021 agattgtgaa tagttaccag cagtcattta cccgcttata acaagcgagg cagttgtaat
    13081 gatagctcag aaggattatg caaggcttcg taagggagaa cgcatatacc acttctgtgc
    13141 atactgttga gctgaaaaac tgacgaatta tgataaactc cagccaactt taattcatat
    13201 cattgagggc ctgtggctga tggcacagct atatttctac tattccgcaa tgaatgcggg
    13261 taagtctaca gcattgttgc aatcttcata caattaccag gaacgcggca tgcgcactgt
    13321 cgtatatacg gcagaaattg atgatcgctt tggtgccggg aaagtcagct cgcgtatagg
    13381 tttgtcatcg cctgcaaaat tatttaacca aaattcatca ttatttgatg agattcgtgc
    13441 ggaacatgaa cagcaggcaa ttcattgcgt actggttgat gaatgtcagt ttttaaccag
    13501 acaacaagta tatgaattat cggaggttgt cgatcaactc gatatacccg tactttgtta
    13561 tggtttacgt accgattttc gaggtgaatt atttattggc agccaatact tactggcatg
    13621 gtccgacaaa ctggttgaat taaaaaccat ctgtttttgt ggccgcaaag caagcatggt
    13681 gctgcgtctt gatcaagcag gcagacctta taacgaaggt gagcaggtgg ttattggtgg
    13741 taatgaacgc tacgtttctg tatgccgtaa acactataaa gaagcgttag aagtcggctc
    13801 attaacggct attcaggaaa ggcatcgcca cgattaataa gaatttcttt actgacaggg
    13861 tgagcagggc gcttttatcc tgtcagttcg ttttactcac tttatccggt gtatataccc
    13921 ttctcgacag ttctttaacg ccgctatacc cctcacagag cgcttttaca gtgacgttga
    13981 cttgtgacct gtaaagtaca atatcgctgt gtgtaggcgt tatacatcgc cgtaaatatg
    14041 atgcaggcaa atgctgtcgg tttatgggaa agttgctttg ggtaaaaaaa acggccccag
    14101 aagggataat gccagtcagt taagcaactg actggctctt tttcggggct gtggggtatt
    14161 tccagggcct ctcctttacc actctcggga aggc
//
LOCUS       DAZGAP010000142        14041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGAP010000142 DAZGAP010000000
VERSION     DAZGAP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.143"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(70..1428)
                     /gene="plaP"
                     /locus_tag="ACHLAM_003754"
     CDS             complement(70..1428)
                     /gene="plaP"
                     /locus_tag="ACHLAM_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO7855214.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1418..1480)
                     /gene="yoeI"
                     /locus_tag="ACHLAM_003755"
     CDS             complement(1418..1480)
                     /gene="yoeI"
                     /locus_tag="ACHLAM_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO7855215.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1695..2624)
                     /gene="yeeY"
                     /locus_tag="ACHLAM_003756"
     CDS             complement(1695..2624)
                     /gene="yeeY"
                     /locus_tag="ACHLAM_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7855216.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2670..3494)
                     /gene="yeeZ"
                     /locus_tag="ACHLAM_003757"
     CDS             complement(2670..3494)
                     /gene="yeeZ"
                     /locus_tag="ACHLAM_003757"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7855217.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3577..3831)
                     /gene="yoeB"
                     /locus_tag="ACHLAM_003758"
     CDS             complement(3577..3831)
                     /gene="yoeB"
                     /locus_tag="ACHLAM_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO7855218.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3828..4079)
                     /gene="yefM"
                     /locus_tag="ACHLAM_003759"
     CDS             complement(3828..4079)
                     /gene="yefM"
                     /locus_tag="ACHLAM_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO7855219.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4362..4412
                     /gene="hisL"
                     /locus_tag="ACHLAM_003760"
     CDS             4362..4412
                     /gene="hisL"
                     /locus_tag="ACHLAM_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO7855220.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4388..4511
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4558..5457
                     /gene="hisG"
                     /locus_tag="ACHLAM_003761"
     CDS             4558..5457
                     /gene="hisG"
                     /locus_tag="ACHLAM_003761"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO7855221.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5463..6767
                     /gene="hisD"
                     /locus_tag="ACHLAM_003762"
     CDS             5463..6767
                     /gene="hisD"
                     /locus_tag="ACHLAM_003762"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO7855222.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6764..7834
                     /gene="hisC"
                     /locus_tag="ACHLAM_003763"
     CDS             6764..7834
                     /gene="hisC"
                     /locus_tag="ACHLAM_003763"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO7855223.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7834..8901
                     /gene="hisB"
                     /locus_tag="ACHLAM_003764"
     CDS             7834..8901
                     /gene="hisB"
                     /locus_tag="ACHLAM_003764"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO7855224.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDLANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8901..9491
                     /gene="hisH"
                     /locus_tag="ACHLAM_003765"
     CDS             8901..9491
                     /gene="hisH"
                     /locus_tag="ACHLAM_003765"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO7855225.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9491..10228
                     /gene="hisA"
                     /locus_tag="ACHLAM_003766"
     CDS             9491..10228
                     /gene="hisA"
                     /locus_tag="ACHLAM_003766"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO7855226.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPARRQIPLIKTLVADVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10210..10986
                     /gene="hisF"
                     /locus_tag="ACHLAM_003767"
     CDS             10210..10986
                     /gene="hisF"
                     /locus_tag="ACHLAM_003767"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO7855227.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10980..11591
                     /gene="hisIE"
                     /locus_tag="ACHLAM_003768"
     CDS             10980..11591
                     /gene="hisIE"
                     /locus_tag="ACHLAM_003768"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO7855228.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11686..12663)
                     /gene="wzzB"
                     /locus_tag="ACHLAM_003769"
     CDS             complement(11686..12663)
                     /gene="wzzB"
                     /locus_tag="ACHLAM_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO7855229.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12810..13976)
                     /gene="ugd"
                     /locus_tag="ACHLAM_003770"
     CDS             complement(12810..13976)
                     /gene="ugd"
                     /locus_tag="ACHLAM_003770"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO7855230.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3537 a         3635 c         3568 g         3301 t
ORIGIN      
        1 aaaaagccgg agcgacaaac tccggctttt tgagatttac cggatgatta tgtcatccgg
       61 tttgtaagat tacgctacgt cttcgtactg cggaaccggg ttacggaaac tcttggttac
      121 gcaagccagg taaatcagac cgatagcagc ccagatcaga cccagaacca ttgagctttc
      181 ttccaggtta acccacagcg caccaacggt cagcgcacca cacatcggca ggaacagata
      241 ctggaagtga tctttcagcg tcttgttacg cttctcacgg atccagaact gcgagatgac
      301 cgacaggtta acgaaggtga acgccaccaa cgcaccaaag ttaatcagcg ccgtagccat
      361 taccaggtcg aagttgattg ccagcagggc aatcgcgcca accaggatga tgttcatcgc
      421 cggagtacgc catttcgggt gtacataacc gaagaagctt ttcgggaaca cgccgtcacg
      481 acccattacg tacatcagac gcgctacgcc tgcgtgcgcc gccataccgg acgccagtac
      541 ggtaatggtg gagaagatca gcgcgccaac ctggaacgct ttacccgcaa cgtacagcat
      601 gatttcaggc tgtgacgcat ccggatcttt aaagcgagag atatccggga agtacagctg
      661 caggaagtaa gttgcaaaga tgaagatcat gccgccgatc agcgcggtca ggaaaatcgc
      721 acgcgggatc acgcgctctg catctttggt ttcttccgac aggttgctga tgccgtcaaa
      781 accggtaaag gagaagcaca ggattgtcgc cccggtaatc atcgggataa catgcgcatc
      841 gccagaccag aacggacgag tgctcgccag cgtaccagcg ccttcacctt caaatacgcc
      901 ataaacaacc atgcccagaa tcaccgcgat cagcactacc tgcaacacga cgattacagt
      961 gttgaagttc gctacggatt tcagactacg caggttaaag gcggtcataa aggccaccag
     1021 cgccaccaca aacatccacg atggaatgga aggcaccaga gcttcaaaat agattttcgc
     1081 cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc agagaagacc aacccaccat
     1141 aaagccgaca gtcgggctaa tggatttctg ggcgtaagtg tatgcagagc cagcagaagg
     1201 atagcggcga accagcttcc cgtagctcag agccgtaaac aggatcgcaa tcaatgcgaa
     1261 cgcataggct gtcggcacat gaccatccgt gaggcctgaa acgataccaa aagtatcaaa
     1321 cagcgtcatc ggctgcatat aggctagacc catcattaca accggaacta acgtaagcgt
     1381 tttacgcaat tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg tcattctcct
     1441 ttacggtgat aaccgtcgcg taagcaaaaa attgccccat ttttttggat tcctcagcga
     1501 caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct cttggtatga
     1561 atgattggtt tgaagcaaaa aataaccgac gctaattaaa cgtcggtttt tagtcatttt
     1621 ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga gaggcttgtc
     1681 ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata aacaccggcg
     1741 atcgcactat ttgctaaaat ttcgtcccgc caccaaccac tggcaagccc tgcggtctgt
     1801 tcattccagc caatccagac gggttcaaag agagtttggg cgaccacttt cttctcgatt
     1861 aacgcgccac tatcgagaaa acgttgcgcc agataccgtg gtaaatagcc gcagccaagg
     1921 ccgctaattt gcagctccag cttggtttta aaatcgaaaa cggtaatggc ttcttgttcg
     1981 tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag tgtcccccac cacaatcgcc
     2041 cgatatcgtt taatgatacg acggtttaac ggttcttctt cctgggctaa cggatgatga
     2101 ggcgcgaccg caaagacttg ttcaagatcg cccaaccgcg agaaaccaaa ctcactggaa
     2161 gatggtggct catgcatcgc gccaacaata atatccgctc gcccctgagt cagtgcatcc
     2221 caggaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg atgttgataa
     2281 aaagcctcaa tgagaggcgc aagcagagaa aaaggaaacg tgtcgtccac gccaatcacc
     2341 agctcatttt cccagccttc atgcagtttg atagcctgct tttccagttc tcgcacggta
     2401 tgcagaactt cccgcccttt ctctaataac atttttccgg tgcgggtgaa tttagcgcgg
     2461 tgaccgctac gatcaagcaa ttgaatatta aggtcgcttt ccagcttgtg aacggtataa
     2521 ctcagagcgg acggcgtctt gtagagttta gccgacgccg ccgcaaaact gccttctttt
     2581 tctaaggcat cgaggatcat caacacatcc agcagtggtt tcatacacgc ccccttgcag
     2641 tgtatgcggc gtcctgctgg cgtgatcgtt tactccagcg gcattaccag cggatcggga
     2701 tactggtatt caaatcccag ttcattacaa atccgactgc catcaataat cttacctttg
     2761 ccgctgtcca gactatttct gaactgcgga ggttccagcc ccagtaaacg ggccatctgc
     2821 ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat gtgtccgcct
     2881 ttaggtgcct gtaacaacag agtgatagca ccaataacat cttctaaatg gactaaatta
     2941 acaccatgtt caccatcagg ggcggttttt ccggcaaaga agcgtccggg atgacgtccc
     3001 ggtccgacca gccccgcaag acgcagaata tcgaccgaag taccgggtaa attgtgcagc
     3061 cagtcttcga gttcttctaa tactcgtcca ctgttggtca ccggattacg cggggtggtt
     3121 tctttcaccg tgccttgcgc gtcgccatag acagatgttg agctggtaaa aataattcgg
     3181 ggaatacgat gggccagcgc gctatccact aactcttgta ccgcttgtaa atagaactca
     3241 tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcgtcggc atccatcagg
     3301 gcatccagat cgtcagaatc gcaaactaac tcaggctcca tgcgaagcag atagctatca
     3361 atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc ggtgacttgc
     3421 cagcctcgcg ctgaaagtga catcgccagc ggcatgccta accaccctaa cccgacaatt
     3481 gcgacctttt tcatccgtta tctcctgata caccttagat ctataaggct acgctagcgt
     3541 atcaaaactg acaattcatt ctatgaatga atctgttcaa taatgataac gacatgctgc
     3601 aatgagtagt gaatcgtcgg taaccgcgta taccagacgg tgctcctctg taatgcgtcg
     3661 ggaccagaaa cctgacaaat tatgtttcag gggttctggt ttccccttac cttcaaatgg
     3721 tgttctgcgg gtatctttga taagttcatt gatcttttta acaattcgct tatctgtttc
     3781 ctgccagtac agataatcat cccatgattc ctcagaccag attagtttca ctcaatgatg
     3841 tccttttccg ttcctttgcc agatttcagg ctatcgattg agtccatcaa tctccgggcg
     3901 ttagcggggg agcgcagtag ataagccgtc tcctccaggg agttgtattc ttcgagtgac
     3961 atcagaacac aagcctctcc attctgacga gtaataagga tcggggcatg atcttcaacg
     4021 gctttcatca ttgttgccga caaattctga cgcgcttcgc tgtagctaat tgtacgcatg
     4081 tcaatctcct cttttgtaca gttcattgta caatgatggg cgttaattaa ctatttatta
     4141 attagtttgt agatcaaggt attgtcagtg agacgaaaat ccaggcttcg ctatttttgg
     4201 tgccgtcagc ttagaggaca gtcctcttag ccccctcctt tccccgctca ttcattaaac
     4261 aaatccattg ccataaaata tataaaaaag cccttgcttt ctaacgtgaa agtggtttag
     4321 gttaaaagac atcagttgaa taaacattca cagagacttt tatgacacgc gttcaattta
     4381 aacaccacca tcatcaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
     4441 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
     4501 ggctttttta ttgcgcggtt gataacggtt cagacaggtt taaagaggaa taagaaaatg
     4561 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
     4621 cgcgaattgc tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
     4681 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgacgacat tcccggtctg
     4741 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
     4801 cttaaccgcc gagcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     4861 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggatgg cccgctctcc
     4921 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     4981 aaaggcatct cttttaaatc ctgcttactg aacggttctg ttgaagtcgc cccgcgtgcc
     5041 ggactggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agcaaacggc
     5101 ctgcgcgaag tcgaagttat ctatcgctcg aaagcttgcc tgatacaacg cgatggcgaa
     5161 atggaagaat ccaaacagca gctgatcgac aaactgctga cccgtattca gggcgtgatc
     5221 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     5281 atcgccctgc tgccaggtgc cgaacgccca actattctgc cgctggcagg tgatcaacag
     5341 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     5401 aaagcgctgg gtgccagttc aatcctggtc ctgccgattg aaaaaatgat ggagtgatcg
     5461 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     5521 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatatcc
     5581 tcgataacgt gaaagcacgc ggcgatgagg ccctgcgtga atacagcgcg aaatttgata
     5641 aaaccacggt taccgcgctg aaggtgtctg ctgaggagat cgccgccgcc agcgaacgcc
     5701 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gaatattgaa accttccaca
     5761 ctgcgcaaaa actgccgccg gtagatgtcg aaacgcagcc aggcgtgcgt tgccagcaag
     5821 tcacgcgccc ggtcgcttca gttgggttgt atattcctgg cggctccgcc ccgctcttct
     5881 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     5941 gctcaccgcc gccgattgcc gacgaaattc tttacgcggc gcagctgtgc ggtgtgcagg
     6001 atgtgtttaa cgtcggcggc gcacaggcca ttgccgcact ggcgtttggt acggaatctg
     6061 tgccgaaagt tgacaaaatc ttcgggccag gtaacgcctt tgtcaccgag gcaaaacgtc
     6121 aggtgagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     6181 tggtgattgc tgacagcggc gctacgccgg atttcgtggc ttctgatttg ctttctcagg
     6241 ctgaacacgg cccggactcg caggtgattt tactgacgcc cgatgctgat atggcgcgtc
     6301 gcgttgccga ggctgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accgcccgcc
     6361 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtagagatct
     6421 ccaaccagta cggcccggag cacctgatca tccagacccg caacgcccgc gatctggtcg
     6481 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcacccgaa tcggcgggtg
     6541 attacgcctc cggcaccaac cacgtactgc cgacttacgg ttacaccgcc acctgttcca
     6601 gcctcgggct ggcagatttc cagaagcgta tgaccgtgca ggaactgtcg aaagaaggtt
     6661 tctccgcgct ggcttcgacc atagaaacac tggccgccgc cgagcgcctg accgcccata
     6721 aaaatgccgt tactttgcgt gttaacgccc ttaaggagca agcatgagca ccgtgactat
     6781 taccgattta gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg cgcgtcgtct
     6841 gggcggtaac ggcgacgtct ggctgaacgc caacgaatac cccacagccg tggagtttca
     6901 gctcactcaa caaacgctca accgctatcc ggaatgtcag ccaaaagcgg tgattgaaaa
     6961 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     7021 aggtattgaa ctgctgattc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     7081 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggcgtcg agtgccgcac
     7141 agtgccgacg ctggacaact ggcaactgga cttacagggc atttccgaca agctggacgg
     7201 cgtaaaagtg gtctatgttt gcagccccaa caacccgacc gggcaactga tcaacccgca
     7261 ggattttcgc actctgctgg agttaacccg cggtaaagcg attgtggttg ccgatgaagc
     7321 ctatatcgag ttttgcccgc aggcatcgct ggctggctgg ctggcggaat atccgcacct
     7381 ggctattttg cgcacactgt cgaaagcttt tgctctggcg ggccttcgtt gcggatttac
     7441 gctggcaaac gaagaagtca tcaaccttct gatgaaagtg atcgccccct acccgctctc
     7501 gacgccggtc gccgacattg cggcccaggc gttaagcccg caggggatcg tcgccatgcg
     7561 cgaacgggtg gcgcaaatta ttgcagaacg cgaatatctg attgccgcat tgaaagaaat
     7621 ctcctgcgtg gagcaggttt ttgactctga aaccaactac attctggcgc gctttaaagc
     7681 ctccagcgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     7741 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     7801 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     7861 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     7921 ctcgcctttg aaccgggcgt gatcccggag ctgctgaagc tgcaaaaagc gggatataag
     7981 ctggtgatga tcaccaatca ggatggtctg ggaacacaaa gtttcccgca ggcggatttt
     8041 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     8101 gtgctgattt gcccgcacct gcccgccgat gagtgcgact gccgtaaacc gaaagtaaaa
     8161 ctggtggagc gttatctcgc tgagcaagcg atggatctcg ccaacagtta tgtgattggc
     8221 gatcgcgcga ccgatattca actggcggaa aacatgggta ttaatggttt acgctacgac
     8281 cgcgaaaccc tgaactggcc gatgattggc gagcaactca ctaaacgaga ccgttacgcc
     8341 catgtagtgc gcaacaccaa agagacgcag attgacgttc aggtatggct ggatcgcgaa
     8401 ggtggcagca agattaacac cggcgttggc ttctttgatc atatgctgga tcagatcgcc
     8461 actcacggcg gtttccgcat ggaaatcaac gtcaaaggcg acctctatat cgacgatcac
     8521 cacaccgtcg aagataccgg cctggcgctg ggtgaagcgt taaaaattgc cctcggcgat
     8581 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgt
     8641 gcactggata tctctggtcg cccacacctg gaatataaag ctgaatttac ctaccagcgc
     8701 gtcggcgatc tcagcaccga gatgatcgag cacttcttcc gttcgctctc ttacaccatg
     8761 ggcgtgacgt tgcacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agagagtctc
     8821 tttaaagcct ttggtcgcac cctgcgccag gccatccgcg tggaaggcga caccctgccc
     8881 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga taccggctgc gccaatttga
     8941 actcggtgaa gtcagccatt gcgcgtcacg gttatgaacc caaagtcagc cgtgacccgg
     9001 acgtcgtgtt gctggccgat aaactatttt tacccggcgt aggcaccgca caagcagcga
     9061 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     9121 tgggcatctg cttagggatg caactgctgg cgcggcgcag cgaagagagc aacggcgtcg
     9181 atttgctggg cattatcgac gaagacgtgc cgaaaatgac cgactttggt ctgcccctgc
     9241 cgcatatggg ctggaaccgc gtttacccgc aggcaggcaa ccgcctgttt caggggattg
     9301 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     9361 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     9421 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagctgctg aaaaacttcc
     9481 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     9541 tcagggagat tatggcaaac aacgcgatta cggtaacgac ccgctgccac gcttacagga
     9601 ttacgccgcg cagggtgccg aagtgttgca tctggtggat ctgaccgggg caaaagatcc
     9661 ggctagacgt caaatcccgc tgattaaaac cctggtggcg gacgttaacg ttccggtgca
     9721 ggttggcggc ggcgtgcgta gcgaagaaga cgtggcggcg ttactggaag cgggcgttgc
     9781 gcgcgtggtg gttggctcca ccgcggtgaa atcaccagaa atggtgaaag gctggtttga
     9841 acgtttcggt gccgatgcct tagtgctggc gctggatgtc cgtattgacg agcaaggcaa
     9901 caagcaggtg gcggtgagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     9961 ggaaacctat cttcccgtcg gcctgaaaca tgtgctatgt accgatatct cgcgcgacgg
    10021 cacgctggca ggttctaacg tctctttata tgaagaagtg tgcgccagat acccgcaggt
    10081 ggcatttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tgcgaggtac
    10141 tggcgtgcgc ggcgtaatag ttggtcgggc attactggaa ggtaaattca ccgtgaagga
    10201 ggctatcgca tgctggcaaa acgcataatt ccatgtctcg acgttcgtga tggtcaggtg
    10261 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc gctggcaaaa
    10321 cgttacgccg aagaaggtgc agacgaactg gtgttctacg atatcaccgc ttccagcgat
    10381 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtaatcga cattccgttt
    10441 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
    10501 gataaaatct ccattaactc ccctgcactg gcagacccga cgttaattac tcgcctggcg
    10561 gatcgctttg gcgtgcagtg tattgtggtc ggcattgata cctggtacga cgccgaaacc
    10621 ggtaaatatc atgtgaatca atataccggc gatgaaagcc gcacccgcgt cactcagtgg
    10681 gaaacactcg actgggtgca ggaagtgcaa aaacgcggtg ccggagaaat cgtcctcaac
    10741 atgatgaatc aggacggcgt gcgtaacggt tacgacctcg aacagctgaa aaaagtgcgt
    10801 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
    10861 gaagccttcc gcgatgccga cgttgacggt gcgctggctg cttccgtatt ccacaaacaa
    10921 ataatcaata ttggtgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
    10981 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacgga ctgatgccgg
    11041 tgattgtgca acatgcggta tccggcgaag tgctaatgct gggctatatg aacccggaag
    11101 ctctggacaa aaccctcgaa agcggcaaag tcaccttctt ctcgcgcact aaacagcgac
    11161 tgtggaccaa aggcgaaacg tcgggcaatt ttctcaatgt ggtgagtatt gccccggact
    11221 gcgacaacga cacgttactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
    11281 ccagcagctg cttcggcgac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
    11341 tgctcgccga gcgcaaatct gccgatccgg aaacctccta caccgccaaa ctgtatgcca
    11401 gcggcaccaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
    11461 caacggtaca tgaccgcttt gaactgacca acgaggcatc tgatttgatg tatcacctgc
    11521 tggtgctgtt gcaggatcag gatctggatt taacgacggt aattgagaac ctgcgtaaac
    11581 ggcatcagtg agttgcgggt gatcagatgc gatgttgttg tcgcatccgg caaaaaaaac
    11641 gggcaaggtg tcaccaccct gcccgttttc tttaaaacca aaagattact tcgcgttgta
    11701 attacgcaga gcgttacgcc ccagcacaat ccccgcgcca accatgccgc ccagcagcac
    11761 tgccagaatc aaggtaattg cctttttcgg gctatcgcga cgaataggta acgtcggttt
    11821 catcacatag cggtaagcat gaatatcaag atcatcaacc tttaagtttt caatatccaa
    11881 caggttttga cgagtctgat agtagtttgg cgagaacacc aacggacgag tcgcctcatg
    11941 cttaatcatc gactccagcg cttcgctccc caaaaggaac aacgtatctt gtgtcacatc
    12001 ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga ttcgcatact gcaacgcttc
    12061 ctgaatctga cggatacgca gatctttctg ctcttgcgcg actacttcct gagttcttaa
    12121 agagtcctgc aagtttttca tacgcagcgc gatgttgtct ttcaggtctt tttccagttc
    12181 ctcattaacc tggtcgtcaa cttgctggat atacttcgcc agctgcttct gagcaccttc
    12241 aggtgtttca ccaacataag acaccgctaa aggtaatgac tgatttttaa cggtaggctc
    12301 aatggtcagc ttttctgctt cttcctgatt atccagcgtt tccgctaatg ctgaaaatgc
    12361 agtgctgtaa cgtccgataa gtgaagcctg aatgtcggag actttcggta cagccgatcc
    12421 gtagatcaca ttcatcgcgt tggtataacc agcgatttgc cccacatcag gttgagtaac
    12481 gatagctgtg gaagtccact tttctttggc aacggccaga taccctatag ccagggcaat
    12541 agcaacaatg acagaaatga tgatagtcat cttgccgcgc cacaactgca ccagtaaatc
    12601 aatcaaatca atctgttccg ggtcatggtt ttgcccagaa acattattat tttctactct
    12661 catcattacc ctaacggacg aaataatttt aagtcgccat agtttagcga taatcgccat
    12721 gaatgctata ggataaatga taaaaagaat gaattattac aaagggaaca tgagagaatc
    12781 agggcgaaaa tcgccctgat aacaggatgt tagtcactgc caaagagatc gcgggtgtag
    12841 actttgtctg ccacatcctt aagctcttct gccatacggt tggaaataat aacgtcggct
    12901 tgttgtttga aggtggcgag atcacgttcc agtcgagagt tgaagaatga atcttctttc
    12961 atcaccggct cgtagatgat cacttcaaca ccttttgcct tgatacgttt cataatcccc
    13021 tgaatggaag acgcgcggaa gttatctgac ccgctcttca taatcagacg ataaataccc
    13081 accacttgcg gtttgcgtga caaaatggca tcggcaataa aatctttacg cgtgcggttt
    13141 gcatcgacaa ttgccgagat caggttattc ggcacagact ggtagttcgc cagtaactgc
    13201 ttggtatctt tcggtagaca ataaccacca tagccaaacg acggattgtt gtaatggttg
    13261 ccaatacgcg gatcgagaca aacaccttcg ataatctgac gagtattcag acctaaactt
    13321 tctgcatagc tatccagctc gttaaagtat gctacgcgca tcgccagata ggtattagca
    13381 aacagtttaa tcgcttctgc ttcagtggag tcggtaaaca gggtcgggat attttgctta
    13441 atcgcgcctt cctgtaataa cgccgcaaaa cgttctgcgc gttctgaacg ctcaccgatg
    13501 acaatacgtg acggatgaag gttatcgtaa agagctttac cttcacggag aaattccgga
    13561 gagaaaataa tattttcagt acgatatttc ttatgcatcg cttcggtaaa accaacggga
    13621 accgttgatt tgatgaccat aaccgcataa ggatttatct caactacgtc tttaattact
    13681 gattctacac tcgatgtatt gaaataatta gttttaggat catagtcggt tggagtggcg
    13741 atgatgacgt aatcagcatc ccgataggct tcatttttat ctaatgtggc attaaagtgt
    13801 attttatctg attgcaaaaa ctgctgaatt tccttatcaa caataggaga tatccgatca
    13861 ttcagcatag caacgcgtga cggtaaaata tctaatgcca caacctcatg attttgtgcg
    13921 attagaagcc cgtttgacaa gccgacatag ccagtaccgg aaatggtgat tttcatttca
    13981 tgctctcaga attaacttaa ctgtgaatca tgatgttttt agcatcctga taagagttaa
    14041 a
//
LOCUS       DAZGAP010000143        14029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGAP010000143 DAZGAP010000000
VERSION     DAZGAP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.144"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..282)
                     /gene="yddM"
                     /locus_tag="ACHLAM_003771"
     CDS             complement(<1..282)
                     /gene="yddM"
                     /locus_tag="ACHLAM_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7855231.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(282..560)
                     /locus_tag="ACHLAM_003772"
     CDS             complement(282..560)
                     /locus_tag="ACHLAM_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7855232.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            complement(746..1756)
                     /gene="adhP"
                     /locus_tag="ACHLAM_003773"
     CDS             complement(746..1756)
                     /gene="adhP"
                     /locus_tag="ACHLAM_003773"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO7855233.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            complement(1890..3587)
                     /gene="maeA"
                     /locus_tag="ACHLAM_003774"
     CDS             complement(1890..3587)
                     /gene="maeA"
                     /locus_tag="ACHLAM_003774"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7855234.1"
                     /translation="MEPKTKRQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            complement(3744..3881)
                     /gene="sra"
                     /locus_tag="ACHLAM_003775"
     CDS             complement(3744..3881)
                     /gene="sra"
                     /locus_tag="ACHLAM_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO7855235.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(3983..4198)
                     /gene="bdm"
                     /locus_tag="ACHLAM_003776"
     CDS             complement(3983..4198)
                     /gene="bdm"
                     /locus_tag="ACHLAM_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO7855236.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            4543..4974
                     /gene="osmC"
                     /locus_tag="ACHLAM_003777"
     CDS             4543..4974
                     /gene="osmC"
                     /locus_tag="ACHLAM_003777"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO7855237.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(5030..5956)
                     /gene="ddpF"
                     /locus_tag="ACHLAM_003778"
     CDS             complement(5030..5956)
                     /gene="ddpF"
                     /locus_tag="ACHLAM_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO7855238.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLTTHGCEVRQSLTIREDEREIRCWRAL"
     gene            complement(5949..6935)
                     /gene="ddpD"
                     /locus_tag="ACHLAM_003779"
     CDS             complement(5949..6935)
                     /gene="ddpD"
                     /locus_tag="ACHLAM_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7855239.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPVLTAYGDNNHRCACWYPQQEVISV"
     gene            complement(6932..7828)
                     /gene="ddpC"
                     /locus_tag="ACHLAM_003780"
     CDS             complement(6932..7828)
                     /gene="ddpC"
                     /locus_tag="ACHLAM_003780"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO7855240.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(7825..8847)
                     /gene="ddpB"
                     /locus_tag="ACHLAM_003781"
     CDS             complement(7825..8847)
                     /gene="ddpB"
                     /locus_tag="ACHLAM_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7855241.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(8849..10399)
                     /gene="ddpA"
                     /locus_tag="ACHLAM_003782"
     CDS             complement(8849..10399)
                     /gene="ddpA"
                     /locus_tag="ACHLAM_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7855242.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSHPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(10413..10994)
                     /gene="ddpX"
                     /locus_tag="ACHLAM_003783"
     CDS             complement(10413..10994)
                     /gene="ddpX"
                     /locus_tag="ACHLAM_003783"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO7855243.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPLAASYPLLADQFTCFISPGTQHAS"
     gene            complement(11252..13651)
                     /gene="dosP"
                     /locus_tag="ACHLAM_003784"
                     /gene_synonym="pdeO"
     CDS             complement(11252..13651)
                     /gene="dosP"
                     /locus_tag="ACHLAM_003784"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO7855244.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSMLPLK
                     I"
     gene            complement(13676..13840)
                     /locus_tag="ACHLAM_003785"
                     /pseudo
     CDS             complement(13676..13840)
                     /locus_tag="ACHLAM_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         3399 a         3653 c         3510 g         3467 t
ORIGIN      
        1 ttgcgtaacc agacggcgca gtcgcgacac atccaccgtt ttttcagcct ctgccaggct
       61 ccaggcgttt tgcagattca gccacatttg cggcgaactg ccgatcacca cggaaagttt
      121 aatcgccatt tccggagtga gcgccgcttt tccggtcagc aatcggcttg ccgttgaggg
      181 cgcaatttcc attgctctgg caaactcgcg caggctgaca ttaagttcgt ccagtgattc
      241 ctgaataatg tcccccgggc ggggatgatt ggccattttc atcagtgata gtcctcgtaa
      301 tccagtatgt atgcatcgcc attgacgaat tcaaatgtga ttcgccagtt gcccgagaca
      361 gatatcgccc aaattccatc gcgatcgccg cttaacgggt gtagtcggta accgggcata
      421 tcgatatcag caagacagct tgctgcatca atcacagcga gtcggtggcg taatcgtttg
      481 acttgcgtcg gtataacgcc ggaggttttg ccgagaagaa acaagtcacg caatcccttg
      541 tgtctgaaat tcattatcat ctcttcatcc ttgttccttc aaaaggaaca ctatcactat
      601 tgttccgtgc agcgcaacaa atttcgctac gtttttgcga attgtgaggg gaaatatggg
      661 ggaggaaaag aagaagagtg aaattgcgga aagcgtctcg caaaacaggc tgcgacccga
      721 acatggcagt cgcagcaaag gcctcttagc ggcggaaatc aatcaccata cggccacgga
      781 ttttgccttc ttccatctcg gtaaagatgg tgttgatgtc cgctaacgga cgcagggcga
      841 ctttcggcac cactttgcct tcggcggcaa actggaaggc ttcggttaga tcctggcgcg
      901 tgccgaccag cgaaccgacc acttcaatac catccagcac aaggcgtggg atgtccaggc
      961 tcatagactc cggcggcaga ccgacagcca caacacgacc gcctgctcgt acagcatcaa
     1021 ccgccgagtt aaacgcagct ttagctaccg ctgtcaccac cgcggcgtga gcgccaccgg
     1081 ttttttcttg cacaattttg gcggcgtctt cagtgcgtga gttaatcgct aaatcagcgc
     1141 ccatttcggt tgccagtttt aactgctcat cattgacatc aatggcgatc actttggcgt
     1201 taaagacatt cttcgcgtat tgcagggcga ggttacccag accgccaaga ccgtagatag
     1261 caatccactg accgggacga atttttgaca gtttcaccgc tttataggtg gtgacccccg
     1321 cacaggtaat gctgctggcc gccgccgagt ccaggccatc tggcactttt accgcgtaat
     1381 cggcgaccac gatgcactct tccgccatcc cgccatcaac gctgtatccg gcatttttaa
     1441 ctgaacggca gagcgtttcg ttaccactgt tacagtattc gcaatgaccg catccttcgt
     1501 aaaaccacgc cacgctggca cgatcgcctg gttttaatga ggtgacacct gggccaactt
     1561 ctgccaccac accgatgcct tcatggccca gaattacgcc ggttttgtca ccaaaatcgc
     1621 cattcttaac atgaagatcg gtatgacata caccacaaca ctccattttc agcagggctt
     1681 cgccatgttt cagtgagcgc agtgttttat ccgtaacgtc aacatgatga tccttcgtaa
     1741 caactgcagc cttcatagtt cctccttttc ggatgatgtt ctgcatagca ggtgaggcaa
     1801 atgagattta ttcgccacta cccagtatgg atgagatctg aaaaagggag aggaaaattg
     1861 cccggcagct ttcaccaccg ggcgcaggct tagatggagg tacggcggta gtcgcggtat
     1921 tcggcgtgcc agaaattatc atcaatggcc tgttgcagag cttcggcaga cgttttcacc
     1981 gccacgcctt gctgctgcgc cattttgcca accgcaaagg caattgcgcg ggagactttc
     2041 tgaatatctt tcagttccgg tagtaccaga ccttcgccgt tcaggaccag cggcgaatac
     2101 tgagcaagcg tttcacttgc cgacatcagc atctcatcgg tgatacgtga cgcgccggaa
     2161 gcaataacgc ccagcccgat gcccgggaaa ataaaggcgt tgttacactg ggcgataggg
     2221 tagattttat ctttccatac cactgggtta aacggactgc cagtggcgac cagcgcgtta
     2281 ccttcagtcc aggcgataat gtcctgcggt gtggcttcca cgcgtgaagt cgggttagac
     2341 agcggcatca cgatcggacg cggacagtgt ttatgcatct cacggataat ctcttccgta
     2401 aacagcccgg tctgtcctga gacgccaatc agaatatctg gttttacatt acgcaccaca
     2461 tccagcagtg acagcacatc gctgtcggta tcccagtcac tgaggttttc gcgcttctgc
     2521 accagtttgg tctggaaagg cagcaggttc ggcatcttgt cggtcagcag gccaaagcga
     2581 tcaaccataa agactttctg ccgcgccgct tcctcgctta atccttcacg ctgagtctgg
     2641 gcgatgatca tttcggcaat gccgcacccc gcagagcctg cgccgaggaa gacaatcttt
     2701 ttctcgctca gttgaccacc tgccgcgcgg ctggctgcga tcagtgtgcc gaccgttacc
     2761 gccgcggtgc cctgaatgtc atcgttaaaa gaacaaattt cattgcgata gcggttaagt
     2821 aacggcatcg catttttttg tgcgaagtct tcaaactgca acagtacgtc cggccagcgt
     2881 tgtttcacag cctggataaa ttcatcaacg aattcatagt attcgtcgtc agtgatacgc
     2941 ggattacgcc agcccatata cagcggatcg ttaagcagct gttggttgtt cgtcccgaca
     3001 tccagcacca ccggaagggt atacgccggg ctgatgccgc cacaggcggt atagagcgac
     3061 agtttaccga tcggaatgcc catcccgccg atgccctggt caccaagccc cagaatacgt
     3121 tcaccgtcag tcaccacaat cactttaata ttatggttcg gcacgttttg cagaatatcg
     3181 tccatattgt ggcggttctg gtaagagata aacacgccgc gtgaacggcg gtagatctca
     3241 gaaaaacgct cacaggctgc gccgacggtt ggggtataaa taacaggcat catctcatca
     3301 agatgattgt ttaccagacg gtagaagagg gtttcgttag tgtcctggat gttacgcagg
     3361 tagatgtgtt tgtcgatttc ggttttgaat ccctgatact ggatccatgc tcgttccgct
     3421 tgttcttcga tggtttcgac cacttccggc agtaacccca gcaggttgaa gttacggcgt
     3481 tcttccatgc tgaaggcgct gcctttattc aacaacggaa attccagcag tacagggcca
     3541 gcgtaaggaa tataaagcga acgctgtctt tttgtttttg gttccatgtc actcactctt
     3601 ttttgaatat ccatccctgg cacgctttta tcgtctttgc tttaccgcca gggcgtcggc
     3661 cccaagtata aagcagataa aaacaaaaac accattgcgc aggcaatggt gtttaatcgt
     3721 cattgaggac tgatggttat gaattacttt tcagcggggc gttttctgcc tgttgggtta
     3781 tttaccacgc tggatttgtc accttcggta actattttgc gctggttaga aattttatgg
     3841 tccagtccaa gaatatgacg tgcctgacgg ttcgatttca ttttaactcc tcaatcctgt
     3901 tactagtttt aaggacaaca tcgccgtagc gaagaaacac gtgctaaacc cctaaattag
     3961 gttgccgatc aagcatagca ccttaaagcg tagggtgctg accactgacc acataattga
     4021 tcgtttgctg gtagatatca ctgaggatgt cgttatcaga tgcttccacc catttggtca
     4081 gctccatgag aatgtcatct tcagtgacaa caccgtgctc tgcccgaagg ccttgctcaa
     4141 tggcattaac cagagcgggt tctgctgttg aattttctgc ctgataataa gtaaacatag
     4201 tgattctccg tgtctgtgta tttatggtgt ctgctacgga tcgcagattt ataaagcaca
     4261 ttcagcatgg caaatatttg ccgcttcgtt gttaagatta gtcctggttg atgattttta
     4321 tattttaact ccatgatatt gataggtatt attattgtga tgagttgatt gatattgatg
     4381 caatatctct tgagttatat atttccggtt atctattgtg gaaatttaat ttaagtgcag
     4441 gaattatatt ttcgccggat tttattcgga ttatcctgct tatcctcgtg ctgtttctca
     4501 cgtagtctat aatttccttt ttaagcccac aggagagcaa caatgacaat ccataagaaa
     4561 ggtcaggcac actgggaagg cgatatcaaa cgcgggaagg gaacagtatc tactgagagc
     4621 ggcgtgctga accaacagcc gtatggattt aacacgcgtt ttgaaggcga aaaaggaacc
     4681 aaccctgaag aactgattgg cgcagcgcat gccgcatgtt tctcaatggc gctttcatta
     4741 atgctggggg aagcgggatt cacgccaaca tcgattgata ccaccgcaga tgtgtcgctg
     4801 gataaagtgg atgccggttt tgcgattact aaaatcgcac tgaaaagcga agttgcggtg
     4861 ccgggtattg atgcctccac ctttgacggc attatccaga aagcaaaagc aggatgcccg
     4921 gtttctcagg tactgaaagc ggaaattacg ctggattacc agttgaaatc gtaaagcgtt
     4981 gccggatgac gcgtcaggcg tgtgattgcc tgatgcgttg ttagcgtctc tacagcgccc
     5041 gccagcaccg gatctcacgt tcgtcctccc ttattgttaa tgattgccgg acttcacatc
     5101 cgtgggttgt cagagggcag cgttcgcgga aaaaacagcc ttgtggcaac gtgcggtttc
     5161 ctggtagatc cgttttacgt aatgcccatt cttcctccag cggtttatca atggcgggga
     5221 gggaatccag caataatcgg gtgtatggat gtgccggagc tgtcagcacc tgttgcgcgt
     5281 ctcctagttc cacaatctgt ccgagataca tcaccgccac ccgatcgctc atatgacgta
     5341 tcaccgagac attgtgtgaa atcagcacat aagtcagctc gcgattttcc tgtagcgtta
     5401 ccagtaaatt gaggatctgc gcctgcacgg agatatccag cgccgaggtt ggttcatcaa
     5461 gcacaatcac gtcaggctgc gaagagagtg ctctggcaat ggcgatacgt tgccgctgcc
     5521 cgccggaaaa cgcatgaggc aagcggtcga gatactccgg acgaataccc acctgcacag
     5581 ccagctcctc tgccaacgct cgccgctgtt gttcactact acgcttagca atccagagcg
     5641 gttccgtgat gatgcgccac accggtaagc gcggattaag cgaagagagc gggtcctgaa
     5701 acaccatctg cataatgcgt tgtgatcctg aacggatgta ctgcccgtgg ctcggttgca
     5761 gcatacccat taaaagctgt gcgagggtgc ttttgccgca gcctgactca ccgacgatcc
     5821 ctaaggtttc accacgacgg atctgtaaat caataccatt gatggcatga acatgttccg
     5881 tagttttacc aagccagttt ttacgggccg ggaaattgat atggacgtcg cgtaacgtta
     5941 ataatgtatc agacactaat gacctcctgc tgcggatacc aacaggcgca gcggtggttg
     6001 ttgtcaccat acgctgttag caccgggacg ttttcacact gtgcacctgc cgcatagcaa
     6061 cgatcgcgaa aagcgcagcc gtcaggcaaa tgggtgaggt ttggtaccgt ccccggaata
     6121 gcgggtaatg gctggcgcgg tattccatgt tccggtgcgc attgcagcaa accaatggta
     6181 tacggatgcc ggggatgatg gataacgtct gccgtcacgc cgctctcaat cacgcttccg
     6241 gcatacatca cgtaaacgct atcgcacagt tgcgacacca cggccatatc atggctgatg
     6301 aacagtaccg cagttccact ggcgcgggct ttatgtttaa gcagacgcag tacctgcaac
     6361 tgtaccgtga cgtccagcgc cgtagtcggt tcgtcggcaa taatcaattg cggctcgcag
     6421 gagaatgcca gcgcaatcat tacccgctgg cgcataccac ctgaaagctc aaacggatag
     6481 cgcgacataa cttccacggc atccgggatt tgcatctctt ccagcagggc aatcgcttta
     6541 gctctggctt cccgacgact tattggttga tgatggcgga tcacgtccat catctgaaga
     6601 cctattcgac gtgtcggatt aagggcggtc atcggttcct gaaagatcat cgccactcgt
     6661 gcgccgcgcc actgacgaag ctgcttttcc cgagcgttaa gaacatcttc tcccagcagt
     6721 gaaatatgtc cccgatgtac gcaataactg cccgtcggta gcagacgcat aatcaacatt
     6781 gcagtgactg atttacccga gccggattct cccaccagac cgacaatttc accgcggttg
     6841 attttcaatg acacattgtt gagcgcgtga acgtcgccgt taaaaccggg gaaactcaaa
     6901 tgcagttgtt gaatgtccag aacgggttgg gtcatgactg ctttcctcct gctttcgggt
     6961 caagcagatc gcgaatacca tcgccaaaga gattaaaccc gacggcggta agcaaaatcg
     7021 ctgcccccgg aaatgcgcaa taccaccatt gatcgagcac atagttgcga ccattcgcca
     7081 ccatcgcccc ccattcagca ctcggttgtt gagcaccaag gccaataaat cccaacgtgg
     7141 cggccattaa aatcgcgcta ccgatatcca gcgatgcctg cacaatcagc ggcggcaggg
     7201 agttacgtaa aatatgccag ttgatcagat gccagcgtga cgcaccaaag gttttcgccg
     7261 cctgaacata ggtatactgg cgaacaacta atgtttgccc ccgcgccagg cgcacataaa
     7321 agggaattcg cacaatagca atcgccagca tggcgttaaa caaactcggc ccgagagcgg
     7381 ctgccagtgc cattgtcagc accagcgaag gaatcgacag cataatgtcc atgatgcgca
     7441 tgataatcgc gtctgcgcgt ccgccaagca caccggatag acacccgagt agcgacccca
     7501 tcatacctgc aatggcgacc accactaatc cggcgagaat tgattgctga ctgccgacca
     7561 gtacgcggct gaacagatcg cgtcccactt catcggtgcc aaaccagtgc gctgcagaag
     7621 gcggcaaaag gcgggcggtt aaatcaatgg cgttgggatc atgcgtcgcc agccacggtg
     7681 aaaaaatcat catcaatagc attaatacaa tgatgactgc gccggtcacg gtaagcgggc
     7741 tgcctttcag catccatacc agttttgcac cgttaaatcg cgtttgtttt ggtgggcgca
     7801 cggctgacgt ttcctcgctt agcatcattc accacctcca cgtccgatac gcggatcaat
     7861 ccacaaatag agcaaatcca ccaccaggtt caccagcaca taagcaaacg aaaccacgac
     7921 ggcaaagccc atcactgccg ggaagtcgag cgcctgtatt gatgttacta cccatgcacc
     7981 cattccgggc caggcaaaaa cggtttcggt gagcactgcg ccatacaaca aatcgcccag
     8041 cgccagtccc aatacggtaa tcgaggggat caacgcattg ggtagcgcat aacataaaac
     8101 gatataccag ccgggcaagc cgctggcccg ggcggtacga atgtaatctt cactcaattg
     8161 ttccagcata gctgagcgga tctggcgagc gacaattccc aggtgaacga acgccagcgt
     8221 taatgccggt aagatgagat gttgcagcgc attgaagaag acttcaccgt tgccttcaag
     8281 cagcgcatcg agcagataaa agccggtaac gtgcgttggt ggatccagcc agtcatcaag
     8341 ccttccgccg ccgggaagaa tttgcagatg accataaaac agcacaatga cgcccagccc
     8401 aagccagaac gcaggtgtgg agattccggt aatggccatt attcgcacca gatgatccag
     8461 ccagcgattt cgccagactg cagagagtat gcccagcggg atgccaatca gaagtgccag
     8521 cagcagggca caaaaagcca gttccagcgt cgccgggaaa aatatgcgca actcttccag
     8581 caccggacgc ccggtacgaa tggatgttcc caggtcaccc tgaaacaggt cgctgacgta
     8641 gcggtaaaac tgtacgtaca gtggctggtc cagtcccagt tgctggcgaa tattatccac
     8701 gatagcatcg ctggcgcggt cacccgccag taaccgcgcc ggatcgccgg ggatcaggtg
     8761 cgagataata aacgttatca cacagacgcc cgccaccacg agcaccagcc cccagcagcg
     8821 ttggcgtaaa atactccaga aggtcacgtt atttactcat ggtattgata ttgaagacct
     8881 gttccagcat gggattgaac acaaagcctt tcacctcttt gttcatcgcc agttggtagt
     8941 ttttctggaa caggtacaca taagcagcgt catcaatgac gattttctgt gcctgctggt
     9001 agtcccgggt acgctgcgtc tggtcggtgg tcgctagcgc attgcgcagt agcttatcga
     9061 cctcactgtt ttcatagaac gagcggttac ccggcagacc ttttttgtct gactcaaacc
     9121 agtaattcat aaacatatac gggtcggcaa aatccggact ccagttgcca atcgcgatgt
     9181 cgtaatcacc tttacccact ctgtcgcgca tggtggcgtt cgccagcttt tccagcttca
     9241 cattgatgcc cagtttgttg agactggatt gtgtcgccag agcgatcggt tcccagttcg
     9301 gatcattatc agaatagaga aacgtcaggc tggtgggttt gctcgtcact ttatcccatt
     9361 cggctttggc tttcgtttcg tcatggttgt attgcattgc cgtcgcatcg tatccccaca
     9421 tgccttccgg aatcgggccg cgcatttgtt ttccgttacc actcagaatg ccgttaacca
     9481 tgccctgata atcggtagac caggaaatgg cccgacgcag atccgcctga ttaagtggcg
     9541 ctttgctgtt attcagatac agataggtga cgcgcagtga cggatactct gccacattga
     9601 ctttattttc ctgcttcagg gcgttgagtt gatccaccgg cagcgcatcg gcaatgtcaa
     9661 tgtcgccacg ggagagctgc aggcgacggg aggcactttc accgataatt tttaccgata
     9721 ctcgtttaaa gttcggttta ttgccggggt aatgcggatt tggcaccaga actaattgct
     9781 gacctttttg ccagcttttc agcataaacg gtccggagcc agcggtattt tgcgcgagga
     9841 aaccacgggc atcatccgcc gcatgctcct ttaagaccgc cggattgata atggatgcac
     9901 cgtcattcgc cagcgtgtag aggaacggtg cgaatgggtg gctaagggta aacttcaccg
     9961 tatgttcgtc gggagcatca atctttaaat ctttgggaaa tgcttctgct ggcccctggc
    10021 cgatttttag taaccgctca aaagagagtt ttacggcttc ggcagtgaca ggtgtgccat
    10081 cggcaaattt agcattgtcc ttcagggtga acgtccactc tttttgatcg tcagacgctt
    10141 tccagctact agccagatcg ccttcaacgt cggttgagcc tttatcaccg tccgttttgt
    10201 actgaaccag tcgctgataa gacgggtagg tcactgtcca gtcgttatta tcaattgtca
    10261 ccgctgggtc gagggtttgt gggtcggcag ctttaccgat caccagcata tctttcggta
    10321 cggcggcgtg cgcaaccggc attgttgtgg caaggacgat cgcgagcaat gtaggacgaa
    10381 acaaaatcga tctcttcatg acagtttcct gattagctgg cgtgctgtgt gccaggggat
    10441 ataaaacagg tgaattgatc agcgagtagt gggtaactcg ctgccagggg taattcgaag
    10501 tgccaccatt cgctcgggat accgacaaag ccaccgccag tcataatcgc attcagcaac
    10561 aggcgattgc gctgagcggc gggcgggacg gaagggtgat aagcatggga acgttcgtgc
    10621 atttcatcga accctgcacc catatcgagg atgttcccgt gctcatcacg aagcgtcagg
    10681 tcgatcgccg tgccacggct gtgattagaa ccgaccgtca catcaacaac atattgcggg
    10741 tctgggcagg cttgccacaa catcgcctgt gcttgttgtg ggcgatacgc atcgtaaatc
    10801 accagttgta agcctgacag ctgggcgata ctgatacttt tcgccagcgc agtaatcgca
    10861 tccttgtgta acagacaacg cgcttgctga taaatagctt tacctgtgat gttatcagcg
    10921 caggcgtatt tcaattcgat ctccagatca gggaagatca cggctaaatc aaccagttcg
    10981 gtggtatccg acataaacga ttcctgtata agacaaaaat tgctgcgctt tccgcttatg
    11041 cagatctcat gccacgccgg gataagcgcc agaatgctgg cttaaagtta tattttttaa
    11101 cttttgatca acatttgtgc agcgtagtgc agttttggtg caagagagga agttaaggaa
    11161 ggaatctccc ggaatcgtag ctgaaatcac agtatttaag tgacagtgtc acgttaaatg
    11221 aaaacccgcg tgtgcgggcg agaggaattt gtcagatttt cagcggtaac atactgctca
    11281 tccagcctgg aatttcttcg gcgggcaggg ggcgggaaaa gaaatatccc tgaataacgc
    11341 gacagtggat cttgcgtagc atctcaaatt gctctttggt ttcgacgcct tccgccacga
    11401 cggttaaatt aaggctttgc ccaatgctgg taatggcttc aagtaaggca aggatgcgtt
    11461 tttcggtcag acaacgatcg acaaaacttt tgtcaatttt gatttccgtt accggaagac
    11521 tgactaagcg ggataatccg gaaaagcccg tgccaaaatc atctaccgat aaaccgatgc
    11581 ccatatcgcg caggatctga atgcgtttaa agatttcggt atcgtgttcc atcatcatgc
    11641 tttccgtgat ttccaccgtc agctggtggc cgtcgatacc ccaggcttgc attgcatcag
    11701 acacctgatt aggcagctga ttactgcgaa agtgcagcgc cgacaagtta acggataacg
    11761 ccgggatatg aatattctgg ctacgccatt ctgctaactg acggcaagct tccgcgatga
    11821 cccagcgccc aatattttcg atttcaccga tctcttctgc gagaggaata aaccgtgaag
    11881 ggggcacatg accatgcagg ggatcgtgcc agcgagcaag ggcttcgatg ccgtacagtt
    11941 cacccgtttc tgcgaagatt tgcggctggt aaaccagttt cagttggtta ttgctaattg
    12001 cttctttcag cgccgcgcct aaaaccaaac gctcttttac catttcgttc atcgccgggc
    12061 tgaagaactg ccagccgtta ccgccatttt tgcgaataaa atccattgca ttgtgagcag
    12121 tggagagcag gtaatcgcgg tttttaccca cgtcgtagct gatgccaatg ctcaaggtga
    12181 gcgggaatgg tttatcgtca atcattatcg gcttgctgac cacattccgt agctcatcgg
    12241 cgatctgcgt aatgttactg acgtcgtttt ccaggctcac gaggacaaac tgcgtacctt
    12301 cgatacgaca gagatattga tccggtttga gtttttcacg aaagcgattg accacttcca
    12361 gcaatgcctg atcggcccac gcatagccaa ggctatcaat cacatcctga atatggtcaa
    12421 caccgatgag atacaccacc ggagagacgg ctttgtcgac caggtcatcg aggtaattgt
    12481 gcaggttatt gcgatttggc agaccggtca tcggatcaaa ttggatgagt tgttcaatat
    12541 gctgacggct tttttcctgt tccagcgcca gcgcggccat atgctggctg atatctgcca
    12601 cgcgttcgat aaaggcgctg gtttctgctc ctgacgaggt tttaatttgc aggatccccg
    12661 caggcgcacc atcacgctga cgaatggtcg ctgaccagct ttgcgcattt tgaacttctg
    12721 caccgtgcga agatgacgcc cagtgtatcg gcatcccgtt gcgcagcgcg aacagcgaaa
    12781 catgcgattc gttgagtaca gattcgatgt tacgacaaat gatttccccc atttcatgaa
    12841 atggcgggct gctgcacatg gcggcgagaa tattgccttc aagctggcgg atttgccttt
    12901 cttcggtgat atccgagaat gtcattacca ggttctgcag atgcgcgagc acgtcataaa
    12961 ccgggctgat agaggcttta atccagattt tttcaccggt gcgcgtcaac agcagaaatt
    13021 cgtcctgatc gcgggcggtt ttccatagca actgctgtaa acgaatacgg ttatcagcag
    13081 ggaattctgg aatgttcagg agtgtatcgg gctgcatacc gctggcttcg ttaatgcagt
    13141 aaccaaacat ttcggtaaat gcgcgattgc actgcacaat atggcgttcc ggatcgagga
    13201 caatcaccgg tcggtcgaga tggtcaacgg caataatcaa ttgtcgggtc tgttcttttt
    13261 gcgccatttc tacgctggca tcccgtacca gcgccaggta ataaactttc ccctcggcgc
    13321 tcactttcga tagcgcaaaa cgggtccaga ttttactgcc gtcttttttc tccagctgca
    13381 gctcccgact catcccctcg acgcgagctt taccaccttc acggttgtga cgaatgtatt
    13441 gaggatgcgc aggacgcaaa tcccgcggaa tcagcatatc aatgttattg ccaatgactt
    13501 cttcacgttt gtacccccag agcttctctg cggcggggtt gaaaaacatc acttcatcat
    13561 tttcgttaat taacaccgcg cccatcatat tttgctcaag ggcggggaaa aaaatgccat
    13621 cggcggcatt atccgcatcg gttagcttca tgattacctc tgcatcctgg cgcatctaaa
    13681 gactggcttt acagagttca acacgggttc tacctcgtct tttggcgata tacagagctt
    13741 catcggctat ttgaatgagg cgctcatagt caggatgacc attaaacatg gcggcaccga
    13801 tggaaagtga gagggcaata tcctcgccgt ttgcggcttt aaaataatgt cgacctttat
    13861 ggttaaaccg caggccaaaa tcaaccaaac acaacgtgcg agatcgacta actgcaccat
    13921 attctcctga aatatgaaga tatactgaaa agaaataagc gacttaggac agtttcaatc
    13981 tacgctactg ttcttgagaa gagtatagcc catcgtaatt atttttcgg
//
LOCUS       DAZGAP010000144        13984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGAP010000144 DAZGAP010000000
VERSION     DAZGAP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.145"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..1150
                     /gene="allD"
                     /locus_tag="ACHLAM_003786"
     CDS             101..1150
                     /gene="allD"
                     /locus_tag="ACHLAM_003786"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO7855245.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1172..2407
                     /gene="allC"
                     /locus_tag="ACHLAM_003787"
     CDS             1172..2407
                     /gene="allC"
                     /locus_tag="ACHLAM_003787"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO7855246.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2418..3203
                     /gene="allE"
                     /locus_tag="ACHLAM_003788"
     CDS             2418..3203
                     /gene="allE"
                     /locus_tag="ACHLAM_003788"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO7855247.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3332..4477)
                     /gene="glxK"
                     /locus_tag="ACHLAM_003789"
     CDS             complement(3332..4477)
                     /gene="glxK"
                     /locus_tag="ACHLAM_003789"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO7855248.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4499..5800)
                     /locus_tag="ACHLAM_003790"
     CDS             complement(4499..5800)
                     /locus_tag="ACHLAM_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO7855249.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTSDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5857..7218)
                     /gene="allB"
                     /locus_tag="ACHLAM_003791"
     CDS             complement(5857..7218)
                     /gene="allB"
                     /locus_tag="ACHLAM_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO7855250.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7279..8733)
                     /locus_tag="ACHLAM_003792"
     CDS             complement(7279..8733)
                     /locus_tag="ACHLAM_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO7855251.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            complement(8903..9781)
                     /gene="glxR"
                     /locus_tag="ACHLAM_003793"
     CDS             complement(8903..9781)
                     /gene="glxR"
                     /locus_tag="ACHLAM_003793"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7855252.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9881..10657)
                     /gene="hyi"
                     /locus_tag="ACHLAM_003794"
     CDS             complement(9881..10657)
                     /gene="hyi"
                     /locus_tag="ACHLAM_003794"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO7855253.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHFTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(10670..12451)
                     /gene="gcl"
                     /locus_tag="ACHLAM_003795"
     CDS             complement(10670..12451)
                     /gene="gcl"
                     /locus_tag="ACHLAM_003795"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO7855254.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMSVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTEACFMHYE"
     gene            complement(12541..13356)
                     /gene="allR"
                     /locus_tag="ACHLAM_003796"
     CDS             complement(12541..13356)
                     /gene="allR"
                     /locus_tag="ACHLAM_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO7855255.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(13434..13916)
                     /gene="allA"
                     /locus_tag="ACHLAM_003797"
     CDS             complement(13434..13916)
                     /gene="allA"
                     /locus_tag="ACHLAM_003797"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO7855256.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT         3672 a         3729 c         3281 g         3302 t
ORIGIN      
        1 ctaactatat ttttttgcct gtctggatca cataatccag atattttccc tgtatgttaa
       61 ttgcagtcat gcttcacacc gtcgttaaaa aggaagacag atgaaaatca gtcgggaaac
      121 actccaccag ctaattgaga ataaactctg ccaggctggg ttaaaacgtg agcacgctgc
      181 aaccgtggct gaagtattgg tttacgccga tgccagaggg atccactctc atggcgcggt
      241 gcgcgtggaa tactacgcgg aacgcatttc aaaaggcggc accaaccgcg aaccggagtt
      301 tcgtcttgag gaaaccgggc cgtgctcggc aattttacat gccgacaatg ccgccggaca
      361 ggtcgcggcg aaaatgggta tggaacatgc catcaaaacc gcccagcaaa atggcgttgc
      421 ggtggtcggt atcagccgga tgggtcacag cggcgcaatc tcttattttg tgcagcaggc
      481 agctcgcgcc gggttaattg gcatttcgat gtgccagtcc gatccaatgg tggtgccgtt
      541 tggcggcgcg gaaatttact acggtactaa cccactggcc tttgccgcgc cgggagaagg
      601 cgacgagatc cttacctttg atatggcgac taccgtacag gcatggggaa aagtgctcga
      661 cgcccgctcg cgtaatatgt ctatcccgga tacctgggcg gtcgataaaa acggagcacc
      721 aacaaccgat ccatttgcag ttaacgccct gctccctgcc gccggaccga aagggtatgg
      781 cctgatgatg atgattgacg tcctctcagg cgtcttactc ggcttaccgt tcgggcgaca
      841 ggttagttcg atgtatgacg atttacacgc cgggcgtaat ttggggcaat tacatatcgt
      901 tattaatccg aactttttct cctccagcga attattccgt caacatctta gccagaccat
      961 gcgcgaatta aatgccatta cccccgcgcc cggttttaat caggtttatt atcccggaca
     1021 ggatcaggat attaaacaac gcaaagccgc cgtcgaaggc atcgaaattg ttgatgatat
     1081 ttaccagtat ttaatttccg acgcgcttta taacacgtca tacgaaacga aaaatccctt
     1141 tgcgcaataa ttattgagac aggactttct tatgattaca catttccgtc aagccataga
     1201 agaaacgctg ccctggcttt cctcttttgg cgctgaccca gcgggtggga tgacccgttt
     1261 actttattcg ccggaatggc tggaaactca gcagcagttt aaaaaaagaa tggcagcaag
     1321 cgggctggaa acacgtttcg atgaagtggg gaatttatac ggtcgcctga gtggcaccga
     1381 atatccacag gaagtggttc tgagcggttc gcatatcgat accgtggtta acggcggtaa
     1441 cctcgacggg caattcggtg cgctggcggc gtggctggca attgactggc tgaaaacgca
     1501 atacggcgcg ccgttacgta cggtcgaagt ggtggcgatg gcagaagaag aaggcagccg
     1561 cttcccgtat gtcttctggg gcagtaaaaa tatctttggg ctggcgaatc ctgacgacgt
     1621 gcggaatatc tgtgatgcca aaggaaatag ttttgtcgat gcgatgaagg cttgcggatt
     1681 tactctgccg aacgccccac taactccgcg tcaggatatt aaagcctttg tcgaactgca
     1741 tatcgaacag ggctgtgtgc tggaaagtaa tgggcagtca attggcgtgg tgaatgcaat
     1801 tgtcgggcag cgtcgttata cggtgacgct gaacggcgaa tcaaaccatg caggcaccac
     1861 gccgatgggt tatcgtcgtg atacggttta cgctttcagt cgcatttgcc atcagtcgat
     1921 cgaaaaagcg aaaaagatgg gcgacccgct ggtgcttacc tttggcaagg tggagccgcg
     1981 tccgaatacg gtgaatgtgg taccgggtaa aaccacgttc accattgatt gtcgtcatac
     2041 cgacgctgcc gtgctgcgcg atttcaccca gcagttagaa aacgacatgc gggcgatttg
     2101 cgatgaaatg gacattggta ttgatatcga tttatggatg gacgaagaac ccgtgccgat
     2161 gaataaggag ctggtcgcca ccctgacaga attgtgtgaa agtgaaaaac agaactaccg
     2221 ggtaatgcac agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg tgccgacctg
     2281 catgattttc attcccagca tcaacgggat cagccataac ccggcggaac gcaccaatat
     2341 taccgacctt gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac ttgcctggca
     2401 gaaataagga gttataaatg ggatatttaa ataacgtcac cggttaccgc gatgatttac
     2461 tggctaaccg tgcgattgtg aaacacggta atttcgcact gttaacacca gacggtctgg
     2521 taaaaaatat tattccgggc tttgaaaatt gtgacgcgac aatcctctcc acgccaaagc
     2581 tgggtgcctc ttttgttgat tatctggtca cactgcatca aaacggcggc aaccaacagg
     2641 gcttcggtgg cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat atcactgcca
     2701 aagccgaagg gaaaacattt gccttaagcg aaggtggcta tctttattgc ccgccaggct
     2761 ccttaatgac gtttgttaac gcccaggccg aagacagcca aatcttttta tataagcgtc
     2821 gctatattcc ggtagaaggt catgcgccgt ggctggtttc tggcaatgcc agcgaactgg
     2881 aacacattca ttatgaaggc atggacgatg ttattctgct ggattttctg cccaaagagt
     2941 taggttttga tatgaacatg catatcctct cttttgcgcc aggtgccagc cacggttata
     3001 tcgaaactca cgtccaggaa cacggtgcct atattctttc cggtcagggg gtttataacc
     3061 tcgacaataa ctggatcccg gtgaaaaaag gcgattacat ctttatgggc gcttactcct
     3121 tacaggccgg ttatggcgta gggcgtggtg aagcgtttag ttatatttac tcaaaagatt
     3181 gcaaccgcga cgtagagatt taagtcatac cactcgcctg attattttta gctatgttgg
     3241 attttgccac ggtttattcc gtggcttttt ttgtagtcaa ttcaggtaaa aaaagatgcg
     3301 atataaatcg catccgcttt aaaggtaaga gtcagttttt aattccctga cctattttaa
     3361 tggcgcaggc aatatttcgc gcgctgttaa agagattggt ttcaccgctg gcgagcactt
     3421 cggctagagg tgccagacgt ggcaaaatgc tgaataccgc gtcaatgccg tactggtgca
     3481 ccacttccac gccatcaccc aatacgccag caatcccaat caccgggaca ttaaactgct
     3541 tcgccaccga cgccacgccc agcggcgctt taccacctgc cgtttgcgag tcgatgcgcc
     3601 cttccccggt gatcaccagt gctgcgccct gcactgcctg cgcaagattg accgcattca
     3661 acacaatctc aatacccggt ttaatatccg cattgaggaa taccgccgcc gcaatgccca
     3721 taccgcccgc cgcgccacca cccgccatct ggcagacgtt gatttcagtt tgctgttgta
     3781 aaacacgggc gtaattttgc agcccctgct ccagctcttc gaccatttcc ggcgttgccc
     3841 ctttttgcgg gccgaacacc gccgccgcgc cgcgtgcccc taccagcgga ttatcaacat
     3901 cacatgcgac ttcaatatgg cagttcgcca gacgcggatc gcattcatcc atctcaatac
     3961 ttgccacgcg tgctaaattg ccgccgttag ccgccagcac ctgaccgtag gcatcaagga
     4021 aacgcacacc gagcgcctgc gccatgccca taccgccgtc aaccgtcgcg ctaccaccaa
     4081 tgccgagaat aatatggcga atgccgttat ccagcgcatg gcgaattaac tcccccgtac
     4141 caaaactgga ggccagcaac ggattacgct tttcaggcgc gaccagcatc aggccacttg
     4201 ctgctgccat ctcaatcacc gccgttttcc cgtcgccggt aatgccataa aaagcattca
     4261 ctttttcgcc catcggcccg cagacttcaa gcttcacgat gttaccgtcc gtcgcggcga
     4321 ccatcgcctc caccgtccct tcgccgccat ccgctatcgg caaacagata tagtgcgcat
     4381 cgggaaagag ggtcgaaaac ccggctttaa ttgcctgaca acatttttct gcacttaagc
     4441 tctctttaaa agagtctggc gcaatgacaa tcttcattat caatcctttt tcaacaaatt
     4501 attcgatgcg ttcccacgga ataagattat ccataagaac agccagtaaa attccgacca
     4561 gtaagccgtt actgagcaga ggacgaagcg ttaatggaag gtcttgcaga tacacaggcg
     4621 gtaatgccat taaaaatatg ccgacaaata acggcaatgc gagtcgataa atattgcgag
     4681 cggtaaacgt gatttgctgg ctaaacacca gcgcggaaaa gagtaaaggc agataagaaa
     4741 ccagcatgac cgcgctactc actggtaaag ggatactgca aaacagtcgc gtgagtgccg
     4801 gaaccagtgc caccagcagg caaataacgc tgccataaat aaacgaacgc cgcgtgtaat
     4861 cgctagtctg ggttaataaa ccaatggatg aaacaaacgg tgaaaatgga attactgcca
     4921 gtggcacggt tatcagtgtc ataaatccgg tcgccacaaa gctgcgacga taacgcgtat
     4981 tccccgcgcc ctgctgcgga taaaaaacat ccgtgccccg aatcgcaccg taggtattgc
     5041 tgatatttac cagacctgta atcaccgccg tcagaataat ccccggcgac aaagcaccac
     5101 cactgccgag cgggaaccac tgccagtgca actcaccgga gagcgaatgc gaagaaggaa
     5161 agcaaaagta ccacaacaac cagccggtta tcgtaccgac cagcaggccg taacgggcaa
     5221 aacgttgtgg caggaaaata atcatcgcca gtaccaggca catcaccgcg accgagagcg
     5281 cgaacggcgg taattgaatt ttaaaattcg ggtcggctat gccaaacggc agcccgagca
     5341 tacctttgaa aaagatagtg gtcagttgcg cacccagcat caacataaac aagaccatca
     5401 ccgacggcgt aaataaccgt gccaggcgat gtcctaatcc gctaaaacca atcaacatcg
     5461 tcagcacgcc ggagagcgca attcccaccg ccagactggt agcgatatcg ttgatcggtg
     5521 tcccgcgcga tgcttcgcca agggtgatag tcaggatggt tccccaccac aggccgcccg
     5581 gcccctccat aatagcgcga cgatgaccgc agaacgcctg agcgaagcag gccagtgcgg
     5641 tagcaagaaa agcatattgc gtgagcgtaa gcaggctact ttgcggcaac tgaaaagcag
     5701 aaagtagcgt aggaggaacc acaaccgtgt tgcaaaaaat aaagaaaaac cactgaaatc
     5761 ctgataacag gctttcgcgg ctgactgcaa aattgaacat aaaaacacct taaccggaga
     5821 atgcccgccg caaggacggg cattgcaggg gccagattac tgctgatgtt taaggataaa
     5881 ttgacctttc ggcgcaacag ggaagccctg ttcgatgtcg taaatcacat caccacgtaa
     5941 gatggttttc gtgatacgcg cgccaatggt acggccaaca tacgggctga ctttgtggcg
     6001 atattccaga tcgtcattgg taagaacata gctgctattc ggctgaatga agacgaagtc
     6061 ggcatctttt cctggggcga tacggccttt ttgctgcaga ccgaaaatat ctgctgcgtt
     6121 agtcgccatt aatttgccga acatcggcag agacataccg cgtttctgta ccgcttcatc
     6181 gaacatcacg tccatgcagc tttgcagacc ggcgatacca ccccatgctt tcatgatatt
     6241 gccggctttc atttccggcg gacatggaga gtggtcggaa accaggcagt cgatttcacc
     6301 gttaaacagt ttttcccaca tgcctttctg gttttccaga tcgcggatcg gcggtgaaca
     6361 cttcgccaga gtaccgattt cttcgaactg atcggtatca agtacaaagt aatgcgggca
     6421 ggattcacaa gtaacgtcct gaccttcctg acgtgcacga gtcacttctt caacgccttc
     6481 cgggctgctg atatggcaaa cgtgcagacg gcaaccggca actttcgcca gatacagcac
     6541 gcggcgaatc gcttccactt cggtaaatac cggacgcgaa gccacatagt catgagcagt
     6601 tacgcgacct tcacgcttcg cttcttcgcc cagttcgtca cagatcagcg cgttttcgca
     6661 gtgcaccagc accggctgcc ccagttcgcc cagcttctgc gcacctttga agaactgcca
     6721 gtcgtttaca tcacggaagt cgttgtcgat accgcgatcg ccacaggtcg caacgaagca
     6781 tttgaagccg acaacgccca cttcatccag ctcatgcaga cggtcgatgt tgtaagacac
     6841 caggccaccg agttgtgccg catcgatagt cagcttgcct ttagcggcat cgaacttcag
     6901 ttcaattgaa gcgcggtcaa ccgttgctgg cagctggttg agcggcattt cgatcatggt
     6961 ggtgatacca ccttttgccg ctgcgcgagt accagtttca taaccttccc agtggctacg
     7021 acccggctca gaaatatggg tgtgcgcatc aaccatgcct ggagaaacca ccagaccaga
     7081 cgcatccatc acttcttttg catcgcccag atcctgaccg atagcagcaa tttttccgcc
     7141 tttaacggcg atatctacaa cgcgagcttc gttttctaaa ataacggtgc cgtttttaat
     7201 gattaaatca aaagacataa caaactcctt gtaaaataca gattggtttt ttaactcaat
     7261 gttcaatatc gggattaatt aacctgtgac tttttgttct cctgtttttt ctgctgctgt
     7321 acgttttttt aataacgcgt aggcagcgaa tgcgacaata acgccgacaa accatgaaac
     7381 gcgtgataaa ggctccatga acggaataaa tttaccaccg agagataaaa taacggcgac
     7441 caaagttact gagaacgcgg taaggttaaa gccgttatcg taatatttga aatcacctgc
     7501 tgcggtatac agttcatcaa gattaatttg tccgcgcatc accataaaat aatgcgccat
     7561 catgacacca attaccggac ccagcattcc gccgataata tcgaggaaca gataaatact
     7621 gtcctgattt tccattaatt tccacgggca gatcaccagg ctgataatac tggcaatcag
     7681 tacgccgttt ttataggtca gtttcgtcgg tgcaatagcg gcaatctgat aaccggccgg
     7741 aataatatta ccggtggcgt tagtggaaat ggtcgtcatc agaataacca gtaccgcaaa
     7801 gaacgaggcg aacaggctgt cccaacgctg aacaatatcc agcacgttcc aggtgtcagc
     7861 gccgtaatga atactggctc cggcaataat acataccccg gcgaccgcaa acagaatata
     7921 ggccacaact aaacccagcg tttgccccag cgcctgctca cgaaacgagt gggcgttttg
     7981 cgtaaagtcg gatgcgctca ccgccggtgc cgcccagacc gcaaccaccg cgtttatcac
     8041 caccagaaac aggaagccac tgttttctgc tttctgaata ccgcccggaa tgtagtcaaa
     8101 gattggaccg atcccgacca gcgaaatcgc ccaaatcgcc ataccgccga aaacgatata
     8161 gatgcacggg ttaagaatgg cagtgaattt atttaaaact tttccgccgc caaaaccaat
     8221 accaacgtta accagccaga agagtaaaaa agtaattaat cccggtagag aaaggcctaa
     8281 cagcgtgaaa tcgccaccga gagtcaaaaa tcccggccag attttgccaa tcagaatcaa
     8341 gcaggccagt gaccccgcgt aacattgcag gccaaaccac ataatggcag caataccgcc
     8401 tcttaataat ccgggaaaca gtgcaccacg tacgccgtaa gaagcacgca ggatcatggc
     8461 aaacggtacg ccgtatttac tgcccgcagc accgtttaat accattaccg cggcaatgaa
     8521 aaaggcactg aggataattg ccagcataat actaaaggta gacaagccga gaataaaaaa
     8581 gccgccaacc atcacataat tgggaacgtt atgaaccgaa cccatccata aggtaaaata
     8641 gttaaatgtt ttccaggtcc gctgactttg cgttttcggc agtagatctt cgctatagcc
     8701 gcgttgctgg aatagttttc tctgatgttc cataaatccc tcttaatttc acgtatcgaa
     8761 taacgttgtt ttaatctagc tatgaggaac aaataaaata aaaatattat ttttaatact
     8821 gaagcaacat ctttggctgc tttctttttt taatattgct ggccgattca ttttctgata
     8881 tcggccattt ttattgcggg tatcaggcca gtttatggtt agccattaat tccagcgcct
     8941 gcactaatgc agagtgatcc aactggctgc caccgtttgc cgcacaggta ttaaacaatt
     9001 cctggcaggt cgcggtgttt ggcaggttca gcgccagtgc tttcgcactt tgcagtgcca
     9061 ggttgagatc tttctggtgc agcgcaattt tgaaacctgg attaaaggtg cgtttaatca
     9121 tgcgctcacc gtgaacttcc agaatacgtg aggaagcaaa gccgcccatc agcgcctggc
     9181 gcacacgtac cgggtccgca ccggcttttg aagcaaacaa caaggcttca gaaaccgctt
     9241 caatattgag cgccacgata atctgatttg ccactttgca ggtttgaccg tcgccgttac
     9301 cgcccacgag agtgatattt ttaccgagca gttcaaacag cggttttaca cgttcaaata
     9361 ctgcttcatc accgccaacc ataatcgata acgtcccttc acgcgcgccg atttcaccgc
     9421 cggagactgg cgcatcgaga taatcgccgc ccagttcatt cacctgacga gcgaaacgtt
     9481 tagtttcaat cggggaaatg gagctcatat caacaatggt tttgcccttc agcgaggctt
     9541 tggtacaacc attttcacca aacagaacgt cttcaacctg tggcgtatcc ggcaccataa
     9601 taaaaatgat gtccgatgca tctgttacct ggcgcgcagt ttcaacacta acggcgccca
     9661 gtgacagtaa ttcatcagca accggaccaa tggttgtgac atgcagttga tgaccggcac
     9721 gcgccagatt aatggccatc ggtgtaccca taatgcctaa gccaataaat cccagtttca
     9781 taattaacct cttttaaatt tcgcttttcc tgaattcaga caacacgatc ccctgccaac
     9841 ttagcggcct aaaaaagcat tgtctgaata gcgttacgtt ttaacggtac ggatccatcc
     9901 agcgtaaacc ggcttccgtg gtggtttggg gtttatattc acagccaacc caaccgttgt
     9961 agtcagaatt ttcaattacc gtaaagagat attcgtaatt aatttctccg gttcccggtt
    10021 cgccgcgatg cggattatca gcaatttgca ggtgaccaat tttatcggcc cactgagtca
    10081 tggtattagt taattcacct tccatccgct gcatgtgata aatgtcatac tgaattttca
    10141 aattgcagca accaacatcg tcaatcagtt tcagtgcctg ccgggttccg gtgaaatgga
    10201 aaccaggaat atcaaaatgg ttaataggtt caatcagtaa taaaatatct tctttcatca
    10261 gcatattcgc agcataacgc aggttttcta caagcgttgt gtgaatctgt tcactgctga
    10321 aaccagtcgg cgttttaccg accagacagt taattttttt attacccaga gcacgagcat
    10381 aacgaatcgc tgctgctacg ccatcccgaa actcttcttc acggccagga atacaggcaa
    10441 taccccgctc gcccgccgcc cagtcacctg ccggtaaatt gtgcagcgta tgttcgagtt
    10501 tattactcgc cagcacctgt tttaattctt caatgtcgta gtcataaggg aacataaatt
    10561 caacgccgcg aaaaccacac tgcgcagctt tctcaaaacg ggcgagaaaa tcatattcac
    10621 caaataacat cgataaatta gcagagaaac gtaacattat ttatctccct tattcatagt
    10681 gcatgaaaca ggcttcagtc ggtgcatccg ctgcgttatc ggcgatatct tcaaattcca
    10741 tgacgttatc cagttcactg cccatcgaaa tattggtcac acgctcgaga ataacttcca
    10801 cgactaccgg tacccgatat tgcgccatta agattttcgc ctgttcaaag gctggtgcaa
    10861 tatcttccgg tttgaaaacg cgaattgctt tacaacctaa accttccgct acttttacgt
    10921 ggtcgacgcc ataaccgttc acttcgctgg agttaatatt ttcgaacgcg agctgtacgc
    10981 agtaatccat atcaaaggca cgttgcgact ggcgaatcag acccagatag gcgttattga
    11041 ccagtacatg gatgtacgga atgttgaact gtgcgccaac ggctaattct tcaatcagga
    11101 actggaagtc gaagtcaccg gaaatcgcca ccacattgcg ctctggatca gcggcacaaa
    11161 cccccagcgc cgccggaatc gtccagccca gcgggcctgc ctgcccacag ttgatccagt
    11221 ggcggtcttt aaagacatgc agcatttgtg ccgcagcgat ttgtgacagg ccaatggtgg
    11281 tgacataaca aacatcgcgg ccaaaggctt tgttcatctc ttcatacacg cgctgcggtt
    11341 tcaccggcac gttgtcgaag tgggttttgc gcagcaaagt gcgtttgcgc tgctggcagt
    11401 cggcgaccca ttctttacga cacggcagac gacccgcttt ttgcatctcc tgcgccactt
    11461 caaccagcag cgtcagcgcc gctttagcat cagagacaat gccgagatcc ggacacagca
    11521 cgcggccaat ttgcgtcggc tcaatatcga tatgaacgat tttgcgccct tcggtgtatt
    11581 tctctaccga accggtatga cggttagcaa aacggttacc gataccaaac accatgtcgg
    11641 acgccagcag cgttgcgtta ccgtaacgat gcgcggtttg cagacccacc atcccggcca
    11701 tcagttcatg atcgtcggga atacagcccc agcccatcag cgttgggatc accggaatgc
    11761 tggtcagttc agcaaactgt tgtaacagtg cagctgcgtc agcattgatt accccgcccc
    11821 cggcaacaat caccggacgt tcggcctgga ttaacatttc taccgctttt tcgatctgca
    11881 tacggctggc agcaggtttg tagaccggca gcggttcgta catgtcagga tcaaactcga
    11941 tttccgcaac ctgaacgtcg aacggtaaat ccaccagtac cggacccgga cgaccagaac
    12001 gcatcaggtg aaatgcctgt tgcagcacgc gaggcaccag cgccgcttca cgtactgtaa
    12061 ccgacatttt gctgaccggt ttagcaattg cttcaatatc aacggcctgg aaatcttctt
    12121 tatgcagacg ggcgcgcgga gcctggccgg taatacacag aataggaatg gaatcagcag
    12181 aagcagaata gagcgcggtg atcatgtcag tgcctgctgg tcccgaggtt cccagacata
    12241 cgccgatatt ccctgccgtt gcgcgggtat aaccttccgc catgtgcgaa gcaccttcca
    12301 catgacgcgc cagaatgtga cgaataccgc cgtgcttacg catcgctgaa tagaaaggat
    12361 tgattgctgc tcccggaacg ccgaaggcgg tggtgatacc ttctttctcc agcacataca
    12421 ttgccgcgtc aacggctctc atttttgcca ttttattcct acctctatta attggaaaaa
    12481 ttttccaact ttcaaatttt gtcaaaaaaa cccgctccgc agagcgggat gcgacagaca
    12541 ctatggatgt gctttcagtc ccaacgccgt gctgatatcg cgggcggtgt ctctgaccag
    12601 ctcaccctga ctgacaaaac gatcttctgt cagtcttgat gaaggcccgg agatggagat
    12661 agcggcaaca acactaccta catcatcgta aattgctgaa gctatgcaat tcaggccgac
    12721 aacatgctct tctttatcta cggtatagcc cagttcacgc gcttgttcca ggtccttcag
    12781 caaggtgggc atatccacaa gcgtagttgg cgtaaactgc tgcaaaccgg tttgcagaat
    12841 gatgctcatc aactcctctt ccgccagcgg ataaagcagc gccttgcccg caccggaagc
    12901 atgcagcggc agacgactgc ccagcggcgc acacatcctg accatcgatt tacactctaa
    12961 ctgaccaatt aataccgctt cattgccgtt acggatcgcg acattgaccg tttcgccgga
    13021 aagtaacatc aggcggcgca taaacggccc ggcgacggag agtacatcgc ggttatggat
    13081 gtacgctgca ccgacgttaa agacacctaa tcctatatgc caccagccta actgactgtc
    13141 ctgatagaca aaatccgctg cctgtaaaac cttcagcaag cgaaaggtcg tggagagcgg
    13201 caaatccaga ttgagagaaa tatcgctaac cgacgaactt cccccacttt tttccagata
    13261 ttgcagaatc gcaattcccc gctctaacgc ctgtgcgccc ttctgtgcca caggctccgc
    13321 ctgtcctggc ctgccgcgcc gtctaacttc cgtcatacct acgttaactc cgacctacaa
    13381 gtgagcataa acaatgaaca attcgtttat cttatgcaag tcggttgcag gcgtcacgca
    13441 aaacagagtt cctgttcagg aatactttca acatcacagt tgtcactgcc gccgcgatcg
    13501 atggtcagaa aatcggtgac gcgctgccag gcgaaaagtg ggtgatgcca gacgttacga
    13561 tggtaattca ccccctgttc accgttggta ataaacgccc gcagcgttga caggtctggt
    13621 ttgtcgtcac ctaacgccac gactaccaca aacacttcac ctttcatcgg gataaacgcc
    13681 tgtgtaccca acggatgacg ttcgagttcg tgaatagtca gcggcagatt cgccggttgc
    13741 gcgcggttaa tgctgataag cgtacggtct tgctcaagaa tctcaaccag cgccaaatcg
    13801 tggtaacgct ctaccaggcc attgttaata tggaaaaaat cccgttgctg cgtttcgatt
    13861 acgtcgccat aagcactaaa ggcttcctga cttaacggta atacctgaag tttcatcttt
    13921 ttgtccttgt aacataattc cgttcaacca ccgccgaccg gtcagcttgg tttcaataac
    13981 aggc
//
LOCUS       DAZGAP010000145        13953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGAP010000145 DAZGAP010000000
VERSION     DAZGAP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.146"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            159..1049
                     /locus_tag="ACHLAM_003798"
     CDS             159..1049
                     /locus_tag="ACHLAM_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855257.1"
                     /translation="MPPHKIRQEWLTLDEAAEVIRQHNITLLPADLIRHALHGDLSLS
                     VYFPSPVLLRPLNPPCRFACRFSKPPFPPSQCNKPGNRKVMANSPPCRRDPTLQLTTS
                     HLWNLPLIGIERLLLHQQLAKTLNRPCPNDTHCCNQIGIVVCDENDSYYQLCCRKSLR
                     ELLLELLATQNNLQEELHLLIGQLYGNGHNDLKSRLSLSVCYLQDTLPPDALFIIRRT
                     HLEAFLTRHQPESKERITSALSRLLWLACKHNPQLDDEMLEHPYKLLNIFECWAREEG
                     MHLPLSPETLKTALQRGAPP"
     gene            1188..1382
                     /locus_tag="ACHLAM_003799"
     CDS             1188..1382
                     /locus_tag="ACHLAM_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7855258.1"
                     /translation="MNSDRFLRLRQVEDKIGFGKSWIYRQIQLQQFPPSIRLNSRHVA
                     WLESEVDAWIHQRIRLTRDV"
     gene            1399..2682
                     /locus_tag="ACHLAM_003800"
     CDS             1399..2682
                     /locus_tag="ACHLAM_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO7855259.1"
                     /translation="MCASHLLNRWLHGASNTGEQEMVQVSLEQIQPFSLNPRVTRNPG
                     YDVLKASILARGLDNPPVLTRRPGDEKYMLASGGNTRLAILNELWQETQDERYHRVCW
                     PFRPWPETLSPQQGEVQCLIGHLAESDLHNGLMFIERAEGVLHLRDLYLEAGIECPTQ
                     LALAEQLTRDGYPVSQSQISRMLQTVDWLLPCIPNALYGGLTRKVIDRLLSLRSAAEQ
                     VWVKLMPLERQPEFADLFSTALAIFNGEPEAVVPQLVQDELLGEMSCRSGMSWNTLLA
                     ELTDGQSKRQVLLGPPAEKVLWPPPPEVEKAPLPEPEPPTEPAAPDEPKPSPLVHTAQ
                     SLCACWGLDACIQPDDSAPAGFTLAPCHDFPHPAAQRCYQVLAALNGDTSTPMPALEL
                     LLSPDYNNAQIQQLMQLISLCRQRRLREVRHDTLS"
     gene            2666..3223
                     /locus_tag="ACHLAM_003801"
     CDS             2666..3223
                     /locus_tag="ACHLAM_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO7855260.1"
                     /translation="MTPSVNQAVLTHIVQALKEGQIRYCESLGFSPQELCELTRLTAD
                     DILFLSNSAVQFITTHIDHEMLGRMLARMEQERLFQQRLEEALSLGASIEFINHYFGL
                     STPEVCARRRLIGLFVRQGRNNAPDEQVETLVWNEWQKTGVKKLDSPEALTAMMAMAR
                     EHDLSLTVIWNLLRQWTQEKLLHEE"
     gene            3213..4085
                     /locus_tag="ACHLAM_003802"
     CDS             3213..4085
                     /locus_tag="ACHLAM_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO7855261.1"
                     /translation="MKSDDRLLFLGNPQQSVPKRLLLARNLSPRDKFAWQLLRLHARD
                     DGSGVFPSYNELQGWLSDQPEGEKASRSTVSNALMMLRITRWLSLCQRLRDEQSGRIT
                     GNLYALHDAPLTHADACRMDSEYLSLLTACSRHRNRRIRNIAIGLLLTLQQPVDGGRT
                     CTESPLKTAPGLNRGLSKKCPSLQLRLSNVAGAGGQPEPHCVHTGTDLNNKHQKRTPD
                     LHWPVDNPFSASERRAAERAMRNLDPALCQQVLDDCLWRIAAHDIHRPLAYLLATLEK
                     ARRGEFNQLRRRRR"
     gene            4245..4856
                     /locus_tag="ACHLAM_003803"
     CDS             4245..4856
                     /locus_tag="ACHLAM_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO7855262.1"
                     /translation="MPGTTLFSDIRITLHTRVAARLWQAHPTGMLLCLALLRRLLRAE
                     EADDPWAAHWLKQLRIRLNLIAHLLKQKNRRLDHAFASLPGAIHTTQVSNPSPTEFIL
                     PLALFSPPGSRLLQQLIDYDLLVRRTLLAWHLGLITQAEKRDFIATIPRLMLQVFSFV
                     NRFRTTGVTRADVRANSPLAQSMAHKLGNLPKKMLTEIQRDVQ"
     gene            4846..5271
                     /locus_tag="ACHLAM_003804"
     CDS             4846..5271
                     /locus_tag="ACHLAM_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HFO7855263.1"
                     /translation="MFNDDDDERSARKSSLKGLLKAFKGKGELRALVQELQALRLQLQ
                     QNQPVLKQAVYELPGRNVPLILCSNPARSGVSYLRWRNLDNNRSGSVALQEAIQQPDA
                     SDELRDALMALEWRRHVLNTQLSVVMFMLRRIQPLTTLV"
     gene            5463..7268
                     /locus_tag="ACHLAM_003805"
                     /pseudo
     CDS             5463..7268
                     /locus_tag="ACHLAM_003805"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            7376..7558
                     /locus_tag="ACHLAM_003806"
     CDS             7376..7558
                     /locus_tag="ACHLAM_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855264.1"
                     /translation="MAKNTGDGYRKGSVTDRTQVQNPQNGNYTKRDTTTGRFIDQKQD
                     GTPFKGVAKEVDDRRK"
     gene            7842..8285
                     /locus_tag="ACHLAM_003807"
     CDS             7842..8285
                     /locus_tag="ACHLAM_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO7855265.1"
                     /translation="MNDTHYFNLYTSGLGYLNRIREVTPKKGEPFLCCDIAALRGEAS
                     NAEHTWFDCRVSGKEAQKLVRRCIDACEQKRKVLIQFRLGDLYADTFVYSKGDRKGET
                     GVSLKAHLLHIGLIKIDGEEVYRAEREAEPQPAPAALPAEPVAQF"
     gene            8359..8739
                     /locus_tag="ACHLAM_003808"
     CDS             8359..8739
                     /locus_tag="ACHLAM_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855266.1"
                     /translation="MNTVLNSREEALDRGKLINLDDEALDAGLPLDLAITLPAHKHCL
                     EDRNDNAIRDLLKGVEMAAVVAFISHQPPQRTIRIDYRSVLTQKTNGSTQQQTVRLTL
                     RVHFSEQGIPIAATLGLMGERFPY"
     gene            8853..9509
                     /locus_tag="ACHLAM_003809"
     CDS             8853..9509
                     /locus_tag="ACHLAM_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilL"
                     /protein_id="HFO7855267.1"
                     /translation="MKRLLTALLLFIFNGVAASPATPEVVRYDRYLLVSTSPQQPPLE
                     QLTTLSVPPGFHPTVGEALQYLLRDSGWSLCLSDARRAQLYGLPLPLNQYHTGPLRLK
                     AALQLLAGPAWRMTADENRREVCFTSDHSLPQTVAYRHPVSLHPLGPLTAPFFLAGTV
                     QRGDIRLAVIVPPEALYLHQVVWLRIGEQWHGWRLARFDERRAVFRHGKRQLTLKVPE
                     "
     gene            9513..10187
                     /locus_tag="ACHLAM_003810"
     CDS             9513..10187
                     /locus_tag="ACHLAM_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO7855268.1"
                     /translation="MKRLILLLLFTASTWAQPLQVQTSQNVGSALQPLTTQQQATVWG
                     ITPEEWQRYLTLKQQARGVWSPGLDPLTTLGVEADTDHERQRFAELLVRQEYQRLEKE
                     LAFQRAYDAAWQRLYPGLTPLKTTSASTSRVSLFVKDDCPPCDSLLRTLLAQQRPLDI
                     WLVGSEGDDNRVRRWAMAHGIDAKLVGEHSITLNHDAGRWLLLGQSKIPVALERKGEQ
                     WREVLP"
     gene            10166..10684
                     /locus_tag="ACHLAM_003811"
     CDS             10166..10684
                     /locus_tag="ACHLAM_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7855269.1"
                     /translation="MARGPALILLLPCLALAQPLPEAYLRTAEHFRVPPELLWAVTQT
                     ESGTNLSGRHLPWPWTLNISGTGYRYASREQACQALLVAIRRTSPKRIDVGLGQINIG
                     WHQAYFDSPCDALYPLQNLSLTAQLLRQRYDERPGSWLNAAARYHRPAGGAPAARYRK
                     LVSRHLQQASPP"
     gene            10681..12699
                     /gene="traD"
                     /locus_tag="ACHLAM_003812"
     CDS             10681..12699
                     /gene="traD"
                     /locus_tag="ACHLAM_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO7855270.1"
                     /translation="MSRYALEALLRPPVEFYSTVIAGCGALICLTQAPLLMLTPWQAR
                     MMASALMLLALRRGWQGWQIVRYQRNLLRLPRYALTSRKIPVSRQRLFLGKGFRWQPI
                     HTQRLYDCLQPEAARWLRPGWCYRLARHIEKRSEHTLPGLARLLSADRWLNPVRPLPP
                     VGGNPWLHGVELKECDVTLPLSERPGHTLVLGTTRVGKTRLAELLATQDIRRGEVVIV
                     FDPKGDADLLKRMWTEAQRAGRGDRFILFHLAYPEFSARYNAIGRFGRISEVASRLTG
                     QLSGEGNSAAFREFAWRFVNIIARALVALGQRPDYQLISRHVVNIDALFIDYARIVLP
                     RHNTRSWEVVVQLAERVNDKNTPRHMLGRDPHVVALEQYLNAEKLYDPILDGLLSAVR
                     YDKTYFDKIVASLLPLLEKLTTGTLAALLAPDYHDLDDARPILDWQQAIRQKAVVYVG
                     LDALSDSVVAAAVGNSMFADLVSVAGHLYKFGLNDGLPGNSEKPPPINLHCDEFNELM
                     GEEFIPLVNKGGGAGIQVTAYTQTLSDIQARIGSAPKTGQVVGNFNNLIMLRVRETAT
                     AELLTKQLPQVEVRSRQVSSGVTDTPGGINGFTSNTCEQVTTRNVPLLDTAALIQLPK
                     GQAFALLEGGQLWKIRLPLPVADKQCPLPDSVAQLAAWMQARQQGLTE"
     gene            12696..13343
                     /locus_tag="ACHLAM_003813"
     CDS             12696..13343
                     /locus_tag="ACHLAM_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFO7855271.1"
                     /translation="MSELRPPPSRPPQGPIGWLLSLAGRIIGLVIGTLVLRVVLELVG
                     LYFWWPQEGSRHVFQVMRQEKVELIIAVQPHPLGKEIRVLLEHGITRVNDVRHKAVSR
                     LFHEPVTVLAYTMISFALRLTWLVIMLPLLCLSAVTGLTEGLVHRDLRRFGSGLESIF
                     LHRYVIRCGSSVTTTLWVVYLAQPFSLPAMLVLLPAALWSGIAVWMAAGSFKRWL"
     gene            13409..13654
                     /locus_tag="ACHLAM_003814"
     CDS             13409..13654
                     /locus_tag="ACHLAM_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO7855272.1"
                     /translation="MGVMSVRLNNNLSTQLEALSKATGRSKSWLANQAIEDYVAREAW
                     QVAEIEQAIQEADAGDFATSDEVDKLFQRLGVKPDGN"
     gene            13644..13928
                     /locus_tag="ACHLAM_003815"
     CDS             13644..13928
                     /locus_tag="ACHLAM_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7855273.1"
                     /translation="MEIKWLRKAIANLEAEFRYIAEDDPQAAERFVNEVWRLTQLLKE
                     QPAMGREGRVPETRELILHNYPYIIPYRVRNNIIQVLRVFHTHRRLPVNW"
BASE COUNT         3010 a         4326 c         3889 g         2728 t
ORIGIN      
        1 tattcgtaat cattgatttc ctaaataaaa cagtaaaaaa cctaaaaaaa acaccaatag
       61 atcacataca aaacaaccag ttaaaagaac tcaccttacc tcgctttagt tggctttttt
      121 agatagtact catcatatgt aactaatagg agttacccat gcccccacac aaaattcgcc
      181 aagagtggct gacgctggat gaggctgcag aagtcatccg gcagcacaac atcacgttgc
      241 tgcccgccga cctgatccgc catgccctgc acggcgatct gtcgctctcg gtctacttcc
      301 cttcgcccgt tctgctgaga ccactcaatc cgccttgccg ctttgcctgc cgattcagta
      361 agcctccttt tccaccttcg cagtgcaaca aacccggcaa ccgcaaagtg atggcaaact
      421 cgccgccgtg cagacgcgac ccaactctgc aactgaccac cagccatctg tggaatttgc
      481 cgttaatcgg cattgagcgc ctgctgctcc accagcaact ggcgaaaacg ttgaatcgcc
      541 cttgccccaa tgatacccat tgctgcaacc agattggcat cgtggtctgc gatgaaaacg
      601 acagctacta ccaactctgt tgccgcaaat cgttgcggga gcttttactg gaactgctcg
      661 ccacgcaaaa caacctccag gaagagcttc atctgctgat tggccagcta tatggtaatg
      721 gccacaatga cctgaaatcc cggctcagtc tgtccgtctg ctacctgcag gacacgctgc
      781 ctcctgacgc actgttcatc atccgccgca ctcatctcga agccttcctg acccgtcatc
      841 agccagaatc aaaggagcgg atcacctccg cgctttcgcg tttgctgtgg ctggcctgca
      901 agcacaaccc gcaactggac gatgaaatgc tggagcatcc ctacaaactg ctgaatattt
      961 tcgaatgctg ggcgcgtgag gaaggtatgc acctgccgct gagccccgag acgttgaaaa
     1021 ccgcactcca gcgcggcgca ccgccgtaac gcgatcaact aaccctttca actaacaact
     1081 ctcactaacc cagataagga tttttactgc ctggcaggga cgctgctgct ttgataacca
     1141 cgcctatcgt gctgatgtga acgaatctgg acacaggagg aatggttatg aatagcgaca
     1201 gatttttacg cttacgccag gtggaagata aaatcggctt cggtaaatcg tggatttatc
     1261 gacaaatcca gctgcaacag tttccgccgt ccattcgact caatagccgc cacgtcgcct
     1321 ggctggaaag cgaggtggac gcgtggatcc accagcgcat ccggcttacc cgcgatgtct
     1381 gaatgccgga ggaagattat gtgcgcttca cacctgctca accgctggct tcacggcgcc
     1441 agcaataccg gagaacagga aatggtccag gtcagcctgg agcaaatcca gccgttctcc
     1501 ctcaatcccc gcgtcacccg caaccccggc tatgacgtgc tgaaggcgtc aattcttgcc
     1561 cgtgggctgg ataacccgcc ggtactgacc cgccgccccg gtgatgaaaa atatatgctc
     1621 gccagcggcg gaaatacgcg cctcgccatc ctcaatgaac tgtggcagga gacgcaggat
     1681 gagcgttacc accgcgtctg ctggccgttc agaccgtggc cggaaacgct gtcaccgcag
     1741 caaggcgaag tccagtgcct gatcggccat ctggccgaaa gcgacctgca taacggattg
     1801 atgtttatcg aacgggcgga aggggtcctg cacctgcgcg atctttacct cgaggcaggc
     1861 attgaatgcc cgacgcagct ggcgctggcg gaacaactca cccgcgacgg ctatcccgtc
     1921 agccagtcgc aaatcagccg gatgctacag accgtagact ggctgctgcc ctgcatccca
     1981 aacgcgctat atggcggtct tacccgcaag gttatcgatc gcctgctgtc actacgcagc
     2041 gcagcagaac aggtctgggt aaagctgatg ccgctggaac gccagcccga atttgccgac
     2101 ctgttcagca ccgccctcgc catcttcaac ggcgagccgg aagccgtggt accgcaactg
     2161 gtgcaggacg aactgctcgg tgagatgagc tgtcgcagcg gcatgagctg gaatacgttg
     2221 ctggcggaac tgactgacgg tcagagcaag cggcaggtgc tgctggggcc tccggccgaa
     2281 aaggtcctct ggccgccacc gccggaagtg gaaaaagcac cactaccaga gcctgagcca
     2341 ccgactgaac ccgccgcgcc cgatgagcca aagccctcac cgctggtgca tacggcacag
     2401 tcgctgtgtg cctgctgggg actggacgcc tgtatccagc cggacgacag cgctccggcc
     2461 ggtttcaccc tcgcgccttg ccacgatttt ccccacccgg cggcacagcg ctgctatcag
     2521 gtgctggcgg ccctgaatgg cgacaccagt acgccgatgc ccgccctcga attgttgctg
     2581 tcgccggact acaacaacgc tcagatccag caactgatgc agcttattag cctgtgccgc
     2641 cagcggcggc tgcgggaggt gcgccatgac accctcagtt aaccaggcag tactcaccca
     2701 catcgtccag gcgctgaagg aggggcagat ccgctactgc gaatcgctcg gcttcagccc
     2761 gcaggagctg tgcgagctga cgcgccttac cgccgacgac atcctgtttc tcagcaacag
     2821 cgcggtgcag ttcatcacca cccacattga tcacgagatg ctgggccgga tgctcgcgag
     2881 gatggagcag gagcggctgt tccagcagcg tctggaggaa gcgctgtcgc tcggcgcgtc
     2941 catcgagttc attaaccact attttggcct ttccacgccg gaggtctgtg cccggcggcg
     3001 gctgattggc ctgtttgtcc gccaggggcg caacaatgcc ccggatgagc aggtggagac
     3061 gctggtgtgg aacgagtggc agaagacggg cgtgaaaaag ctcgactcac ccgaagcgct
     3121 aaccgccatg atggcaatgg cccgcgaaca cgatctgtcg ctgacggtca tctggaacct
     3181 gctgcgccag tggactcagg agaagctgct gcatgaagag tgacgatcgg ctgctgtttc
     3241 tcggcaaccc gcagcagagc gtgccgaagc gcctgctgct ggcgcgcaac ctctccccgc
     3301 gcgacaagtt cgcgtggcaa ctgctgcgcc tgcatgcccg cgacgacggc agcggcgtct
     3361 tcccctccta caacgaactg cagggctggc tctctgacca gcctgaaggc gagaaggcct
     3421 cgcgcagtac cgtcagcaat gcactgatga tgctgcggat cacccgctgg ctgagcctgt
     3481 gccagcggct gcgcgacgag caaagcggca ggatcaccgg caacctctac gccctgcatg
     3541 atgcgccact gactcatgcc gatgcctgtc ggatggacag tgaatacctg tcgctgctca
     3601 ccgcttgttc ccgccaccgc aaccgacgca tccgaaacat cgctatcggg ctgctgctca
     3661 ccctgcaaca acctgtggac ggtggacgaa cctgcactga gtctccattg aagaccgccc
     3721 ctggtcttaa tcgaggactc agtaaaaaat gcccgagtct tcaattaaga ctcagtaatg
     3781 tggcgggggc tggcgggcag ccggagccgc attgcgtaca tactggtaca gatttaaaca
     3841 ataaacatca aaagcgtacg ccagacctgc actggcctgt ggataatccc ttcagcgcca
     3901 gcgagcgccg cgccgcagaa cgcgcgatgc gcaatctgga tcccgctctc tgccagcaag
     3961 tgctggacga ctgcctgtgg cggatcgccg cccacgatat ccaccgcccc ctcgcctatc
     4021 tgctggcaac gctggagaaa gccagacggg gcgagttcaa tcagcttcgg cgcaggcgca
     4081 gatagccctg attttatgcg caccgcataa agccggtgta ccaactatca atcaaacctc
     4141 attcatcggc gaaattaact ttctgcggac cagatgggtt tcccgtcgcc tgactcattg
     4201 tgcggaccga cactgcgggc atctgaatcc atggagaatt tccgatgcct ggcacaaccc
     4261 tgttcagcga tatccgcatc accctgcata cccgcgtcgc cgcccggctg tggcaggcgc
     4321 atcccactgg catgctgctc tgtctggcgt tactcaggcg tctgctgcgc gccgaagagg
     4381 ccgacgatcc gtgggccgcc cactggctga agcaactgcg catccgcctg aacctgatcg
     4441 cccacctact gaagcaaaaa aaccgtcgtc tggaccatgc tttcgccagc ctgccaggcg
     4501 ctatccacac cacgcaggtc agtaacccgt ccccgaccga attcatcctg ccgctggcgc
     4561 tattctcacc gcccggttcg cgcctgctgc aacagttgat tgattacgat ctgctggtcc
     4621 gccgcaccct gcttgcctgg catctcggcc ttatcaccca ggcggagaag cgcgatttca
     4681 ttgccaccat cccacgcctg atgctccagg tgttcagctt cgtgaaccgc tttcgcacca
     4741 ctggcgtgac tcgcgccgac gtgcgggcga acagtccgct ggcgcagtcg atggcgcata
     4801 agctcggaaa tttgcccaaa aagatgctta cggagataca gcgcgatgtt caatgacgat
     4861 gatgatgaac gctccgcccg caaaagctcg ctaaagggcc ttttaaaggc ctttaagggt
     4921 aagggagagc tgcgcgcgct ggtacaggaa ttacaggcgc tgcgcttgca gctgcaacaa
     4981 aatcagccag tgctgaaaca ggcggtgtat gaactgccgg ggcgtaatgt accgctgatt
     5041 ctgtgcagca acccggcgcg ctccggcgtc agctatctgc gctggcgcaa tctggacaac
     5101 aaccgttcgg ggagcgtggc gttgcaagag gccattcaac agccggacgc gagcgatgaa
     5161 ctgcgcgacg cgctgatggc gctcgaatgg cggcggcatg tgctgaatac gcagctttca
     5221 gtggtgatgt ttatgctgcg gcggatacaa ccgttaacca ccctggttta aaattttgcg
     5281 caaagttaaa ccctgagtgt cactttatcg attattttaa acttaactca tgcaccttaa
     5341 aacaggcact gtgcgcttaa tgcacgtttt aaggtgcaat ttttaccttc tcctaccagt
     5401 ttcccctgtc gccttatccc tccggcacac catgctgaac tccctttcac cggagacagc
     5461 ccatgcaact ttacctgtgc gaaaaaccct ctcaggcacg cgacatcgcc cgaattcttg
     5521 acgcccataa ccgcagcgac ggctacctgc ataacgccac cgtaaccatc acctggtgtt
     5581 tcggccatct gctgagcatg gccgcgccgg aggattacga tccggcgctg aaacactggc
     5641 ggacggaaca gctgccgttt attcccccgg catggcggtt ggtgatccgc ccggaggtgc
     5701 ataagcagtt ccgtatcatc gaaaagctgc tgaaacaggc ggatgcagtg gtgattgcca
     5761 ccgacgccga tcgcgaaggc gaaaccatcg cccgcgaaat tctggaacaa tgccgctggc
     5821 gcggtccggt ccagcggctg tggctgtcgg cgctggatga ggccagtatc cacaaggcgc
     5881 tcgccactct gcgtcccggc gacagcacct ttccgctgta tcaggccgga ctggcgcgcg
     5941 cccgcaccga ctggctggtc ggcatcaaca tgacccgcgc ctgcacgcta atgaaccggc
     6001 ggcacaaggg cgtgctctcc gtgggccggg tgcagacgcc gaccctgcgt ctggtggtgg
     6061 agcgcgaccg ggagatcgcc cgcttcacgc cccgcccctg gtggcgggtg gatgcccgtt
     6121 tgcatgcgga gggttgttcc tttctggcgc agtggcagcc ggatagtgcg tactgtgacg
     6181 acgaaagccg ctgcatccgc gaatccgccg cccgtgaggc cacagcacgc ctgcaacaag
     6241 ccggaaccgc gctggtaatg gacgtgacaa cccggcggga gaaaacgccg ccaccgcagg
     6301 ccttcactct cagcgccctg cagcaggcct gctcaaaaca gtggggcatg ggcgcacagg
     6361 aggttctgga tatcgcccag ctcctgtatg aaacccataa agccaccacc tacccgcgca
     6421 ccgactgcgg ctatctgccg ctctccatgc acgatgaagc accacaagtt ctggcggcgc
     6481 tggtcgccag cgacgcttca ctccagcccc tcgccacgca gcttaatctg cagcaacgct
     6541 cccggttgtg ggacgacagc aaaatcaccg cccaccatgg catcatcccg acccgtaaaa
     6601 cggtagatct gacgcagatg aacgacagcg agcgcaaggt ctacggcctg gtacgcagcc
     6661 attttctggc gcagtttctg ccagaccacg aagtggataa aacggacctg cgcctgaact
     6721 gcgccgggga aacgctgact gcccgtggct cagtggtgat tgtcagcggc tggcgacaac
     6781 tgtttttgcg cgaaatggca acgcactcac cggaagaaac gcaggcactt cccgccctgc
     6841 aacagggaca aacctgctag gtggaacagg tggacgttcg cgcccgccag acccagccac
     6901 cggagcacta caccgaaggg acgctgattg ccgcgatgaa aaatgccgcc cgttttgtca
     6961 gcgatgaacg cctgaaacag cggctgaaag aaaacgcggg catcggcacc gaagccaccc
     7021 gcgccgggat tatccagacg ctccttaagc gcggctatct gattaagcag cgtcgtttcc
     7081 tgctggcaac ggataccgcc agcacgctga ttgacgccct gccggaaacg ctgaaagatc
     7141 caggcaccac tgcactgtgg gagcaaatgc tggatgacat cgcgacgggg aaaatgggac
     7201 tggaggcgtt cctggtgcag caacagcaga acgtgacaga gctgattaag tcgttacgga
     7261 atgagtaaat aacctgtttc ctgcatcaca cttttacttt tccctgattg ctcgttcaaa
     7321 ttggcagtct gatgatcctc tctgaggaat attcctcatt tttcggagtg ttaagatggc
     7381 caagaatacc ggagacggtt atcgcaaggg ctctgttaca gaccgcacac aggttcagaa
     7441 tccgcagaat ggcaactaca ccaaacgcga tacaacgaca ggtcgcttta tcgaccagaa
     7501 gcaggacggc actcccttta aaggtgtcgc caaagaagtc gatgaccgcc ggaaataata
     7561 tttagctaac tttcagggcc ttagttatta aggccttttt gcattctgta cttccaactt
     7621 tcacgccgta aaaacaattc cacctttcgt ttctccctac cgaagattca actaaacagg
     7681 tcaaccccaa aacccgccat gcattcctga aacgcggagg cgcacagcgc ggaatgcccg
     7741 tactgccagt aactcagagg ccttgcagcc aatgagccaa acccgaagcg gggcgttccc
     7801 gcgcatgggg cgctccgcac cttcaacacg gagataccac catgaacgat acccactact
     7861 tcaacctgta caccagcggt ctgggctacc tcaaccggat ccgggaagtg accccgaaaa
     7921 aaggcgaacc gttcctgtgt tgcgacattg ccgcactacg gggcgaagcc agcaacgcgg
     7981 agcacacctg gttcgactgc cgcgtcagcg gcaaggaagc gcagaaactg gtgcgccgct
     8041 gtatcgacgc ctgcgagcag aaacgcaagg tgctgatcca gttccgcctc ggcgatctgt
     8101 acgccgacac ctttgtctac agcaaaggcg accgtaaagg cgaaaccggc gtcagcctga
     8161 aggcccatct gctgcacatc gggctcatca aaattgacgg tgaagaggtt taccgggcag
     8221 agcgcgaagc tgaacctcag ccagcgccag ccgcactgcc agcagagcct gtagcgcagt
     8281 tctgacgcac agcattcacc ctgccgggga aattccccgt cggggcgttt ccctggcctt
     8341 ttaccggaga gacgcacgat gaataccgta ttgaactcac gcgaagaagc ccttgaccgg
     8401 ggcaaactga ttaaccttga tgacgaggcg ctggacgctg gcttgccact ggatctggcc
     8461 attacccttc cggcccacaa gcactgtctt gaagaccgga atgacaacgc catcagggac
     8521 ttgctgaaag gtgttgaaat ggcagcggta gtggccttta tctcccacca accgccgcag
     8581 cgcaccattc gtatcgatta ccgctcggtg ctgacgcaaa aaaccaacgg cagcacgcag
     8641 cagcaaaccg tcagattgac gttacgcgtt catttttctg agcaaggcat ccccatcgcc
     8701 gccacgctgg ggctgatggg ggaaagattc ccttactgat ctgattttgc gcccggaata
     8761 acgggcgcta ttttttgacg cagcaaacag ttgtcggtag tccccagccc tctcatccgg
     8821 cacgctcctg taaaagcact gtggaggatg cgatgaaacg actgctgaca gctctgctcc
     8881 tgtttatttt caacggcgtg gccgcctccc ctgcaacgcc agaagtcgtc cgctacgacc
     8941 gctatctgct ggtcagcacg tcaccgcaac aaccgccact cgaacagctt accaccctgt
     9001 ccgttccgcc cggttttcac ccgacagtcg gtgaagcatt gcagtacctg ctgcgcgaca
     9061 gcggctggtc tttgtgtctc tccgatgcgc gccgggcgca gctttatggc ctcccgttac
     9121 cgttgaacca gtaccacacc ggcccgctgc gcctgaaggc cgccctgcaa ctgctggcag
     9181 gcccggcatg gcgaatgacc gcagacgaaa accgccgtga agtctgtttt accagcgacc
     9241 attccctccc ccagacggtg gcttaccgtc atcccgtgtc cctccaccct ttggggccgt
     9301 taacggcccc gttttttctc gccggaaccg tacagcgcgg cgatatccga ctggcagtga
     9361 tcgtgccgcc ggaggccctt tatctccatc aggtagtgtg gctgcgtatc ggggagcagt
     9421 ggcacggctg gcggctggcg cggtttgatg agcgccgggc ggtattccgc cacggtaaac
     9481 gccagctgac gctgaaagta ccggagtaag cgatgaaacg cctgattctc ctgttactgt
     9541 tcaccgccag cacgtgggcg caaccgcttc aggtgcagac cagccagaac gtgggttcag
     9601 ccctgcaacc actgaccacg cagcagcagg ccaccgtctg gggcatcacg ccagaggagt
     9661 ggcagcgcta tctcacgctg aaacagcagg cgcgcggcgt ctggtcgccg gggctcgatc
     9721 cactgaccac actcggcgta gaagccgaca ctgaccacga acgccagcgc tttgccgaac
     9781 tgctggtcag gcaggagtac cagcggctgg agaaagaact cgccttccag cgtgcttatg
     9841 atgccgcctg gcagcgcctg tatccgggcc tgacgccgct gaaaaccacc tctgccagta
     9901 cttcccgcgt ttcgctattt gttaaagatg actgcccgcc ctgcgacagc ctgctgcgta
     9961 ccctgctggc gcaacagcgc ccgctggata tctggctggt tgggagtgaa ggcgatgata
    10021 accgggtgcg ccgatgggcg atggcgcatg gcatcgacgc aaaactggtg ggtgaacaca
    10081 gcatcacgct taaccacgac gcgggccgct ggctcctgtt ggggcagagc aaaatcccgg
    10141 tggcgctgga acgtaagggg gagcaatggc gcgaggtcct gccctgatcc tgctgctacc
    10201 ctgtctggcg ctggcacagc cgctgccaga agcgtacctg cgcaccgcgg agcatttccg
    10261 cgtgccgccg gagctgctgt gggccgtcac acaaaccgaa agcggcacga acctgagcgg
    10321 cagacatctt ccgtggccgt ggacgctcaa tatcagcggg accggctacc gctatgccag
    10381 ccgggagcag gcctgccagg cgctgctggt tgccatccgc cgcaccagcc caaagcgcat
    10441 tgacgtggga ctcgggcaaa tcaacatcgg ctggcaccag gcatattttg actctccctg
    10501 cgacgccctg tatccccttc aaaacctgtc gctcaccgcg caactgctgc gtcagcgcta
    10561 cgatgaacgt cccggaagct ggctcaatgc cgccgcccgc tatcatcgtc ccgctggcgg
    10621 cgcgcctgcc gcgcgatacc gcaagctggt cagccgccat ctgcaacagg cctcaccgcc
    10681 atgagccgct acgcgctgga ggcgctgctg cgcccacccg tggagtttta cagcaccgtc
    10741 atcgctggct gtggcgcatt aatctgcctg acgcaggccc cgctgctgat gctgacgccg
    10801 tggcaggccc gcatgatggc cagtgccctg atgcttctgg ccctgcggcg cggctggcag
    10861 ggatggcaaa tcgtacgcta ccagcgtaac ctcctgcgcc tgccgcgcta cgcgctcacc
    10921 agccggaaaa tccccgtcag ccgccagagg ctgtttctcg gtaaaggctt tcgctggcag
    10981 cccatccaca cacagcgtct gtacgactgc ctgcaaccgg aagccgcacg ctggctgcgc
    11041 cccggatggt gttaccgcct ggcaagacac atcgaaaaac gcagcgagca taccctgccc
    11101 ggccttgccc gactgctcag cgccgatcgc tggctgaacc cagtacgtcc gctgccaccc
    11161 gtaggcggca atccgtggtt gcatggggtg gaactaaaag agtgcgacgt cactctgccg
    11221 ctcagtgaac gccccggcca tacgctggtg ctcggcacca cccgcgttgg caaaacccgg
    11281 ctggcggaac tgcttgccac tcaggacatc cgccgtggcg aggtggtgat cgtttttgat
    11341 ccgaaaggcg acgcggatct gctgaagcgt atgtggacag aagcgcagcg cgccggacgc
    11401 ggcgaccgct ttatcctgtt ccatctggcg tacccggagt tctccgcccg ctacaacgct
    11461 atcgggcgat ttggccgcat ttcggaggtg gcgtctcgcc tcactggtca gctttccggc
    11521 gaaggcaact cggcggcctt tcgcgagttc gcctggcgct ttgtcaacat catcgcccgc
    11581 gccctggtgg cgctcggcca gcgccccgac tatcagttaa tttcccgcca cgtcgtcaat
    11641 atcgacgccc tgtttatcga ctatgcccgc atcgtgctgc cgcgccacaa cacgcggtca
    11701 tgggaagtgg tggtgcaact ggcggagcgc gttaacgaca aaaacacgcc gcgccacatg
    11761 ctgggccgcg atcctcacgt ggtggcgctg gagcagtacc tcaacgcgga aaaattatac
    11821 gatccgatcc tcgatggcct gctctcggcg gtgcgctatg acaaaaccta tttcgacaag
    11881 attgtcgcct cactgctgcc actgctggaa aagctgacca ccggcacgct tgccgccctg
    11941 ctggccccgg attaccacga tctggacgac gcgcgtccga tcctcgactg gcaacaggcg
    12001 atccgccaga aggcggtagt ctatgtcggg ttggacgcgc tctccgattc ggtggtcgct
    12061 gccgccgtcg gcaattcgat gtttgccgat ctggtgagcg tcgccgggca tctgtataaa
    12121 tttggcctca atgatggact gcccggcaat agcgaaaaac cgccgcctat caatctgcac
    12181 tgcgatgagt ttaatgaact gatgggcgag gagtttattc ccctggtcaa caagggcggc
    12241 ggtgcgggca ttcaggtcac cgcctacacg cagacgttga gtgatattca ggcccgtatc
    12301 ggcagcgcac caaagacagg ccaggtggtg ggtaacttca acaacctgat tatgctgcgg
    12361 gtacgcgaaa ccgccaccgc cgaactgctg acgaagcaac tgccacaggt ggaagtgcgc
    12421 agccgccagg tcagttcagg tgtcaccgac acgccgggcg gcatcaacgg ctttaccagc
    12481 aacacctgcg agcaggtgac cacccgcaat gtaccgctgc tggataccgc cgcgctgatc
    12541 cagctcccca aaggccaggc ttttgccctg ctggaaggcg ggcagttgtg gaaaattcgt
    12601 ctgccgctgc ccgtagcgga taagcaatgc ccactaccag attctgtcgc gcaactggcg
    12661 gcgtggatgc aggcgcgcca gcaggggcta acggaatgag tgagctgcga ccgcctccgt
    12721 cacgcccacc acagggaccg attggctggc tattgtcact ggccggacgg attattgggc
    12781 tggttatcgg tacgctggtg ctgcgagtgg tgctggaact agtggggcta tatttctggt
    12841 ggccgcagga agggagcagg catgtttttc aggtgatgag gcaggagaag gtggaactga
    12901 ttattgcggt acagccacat cctttgggga aagagatacg agtactgctg gagcatggaa
    12961 taacccgcgt taatgatgtc agacataagg ccgtgagtcg gttgttccac gaacccgtta
    13021 ccgtcctggc ttataccatg atcagctttg cgctgcgcct gacgtggctg gttatcatgt
    13081 tgccactgct gtgcctcagt gctgtaaccg ggctgacaga aggcctggtg caccgggatt
    13141 taagacggtt tggctccggg ctggaatcca tttttctcca ccgttatgtc atcaggtgcg
    13201 gcagttcagt caccaccacg ctttgggtgg tttatctggc gcagccattt tctctgcccg
    13261 cgatgctggt tttacttcct gccgccttat ggtccgggat agcggtatgg atggcagcag
    13321 gaagctttaa acgttggtta taagttgctc aaaaaatcgc acctcactac aatgtataca
    13381 atgtgtacat gtgtatgagg ccaccaatat gggcgtaatg tcagtccgtt taaacaacaa
    13441 tctttccacg caactggaag cattatcaaa agcgaccgga cgctcaaagt cctggctggc
    13501 aaatcaggca attgaagatt atgttgcccg ggaggcctgg caagttgcag agattgagca
    13561 ggccattcag gaagctgatg ccggtgattt tgccacttca gatgaggtgg ataagctgtt
    13621 tcaacgtctg ggagtaaagc cagatggaaa ttaagtggct acgtaaagcc attgccaatc
    13681 tcgaagctga gttccggtat attgctgaag acgatccgca ggctgcggaa cggtttgtga
    13741 acgaagtctg gcgacttacg caactgctca aagagcaacc cgctatgggc cgggaaggaa
    13801 gggtacctga aacacgtgag ctcattcttc ataattaccc ttatatcatt ccctatcgag
    13861 tacgaaataa tataattcag gttttacgcg tgtttcatac ccatcggcgc ttacctgtaa
    13921 attggtgata aaaaacacaa gacaagaaat ttt
//
LOCUS       DAZGAP010000146        13862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGAP010000146 DAZGAP010000000
VERSION     DAZGAP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.147"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..1193
                     /gene="citC"
                     /locus_tag="ACHLAM_003816"
     CDS             135..1193
                     /gene="citC"
                     /locus_tag="ACHLAM_003816"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO7855274.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1208..1504
                     /gene="citD"
                     /locus_tag="ACHLAM_003817"
     CDS             1208..1504
                     /gene="citD"
                     /locus_tag="ACHLAM_003817"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO7855275.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1501..2409
                     /gene="citE"
                     /locus_tag="ACHLAM_003818"
     CDS             1501..2409
                     /gene="citE"
                     /locus_tag="ACHLAM_003818"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO7855276.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTDLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2420..3952
                     /gene="citF"
                     /locus_tag="ACHLAM_003819"
     CDS             2420..3952
                     /gene="citF"
                     /locus_tag="ACHLAM_003819"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO7855277.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVYLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3956..4507
                     /gene="citX"
                     /locus_tag="ACHLAM_003820"
     CDS             3956..4507
                     /gene="citX"
                     /locus_tag="ACHLAM_003820"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO7855278.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKLGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4482..5360
                     /gene="citG"
                     /locus_tag="ACHLAM_003821"
     CDS             4482..5360
                     /gene="citG"
                     /locus_tag="ACHLAM_003821"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO7855279.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5411..6874
                     /gene="citT"
                     /locus_tag="ACHLAM_003822"
     CDS             5411..6874
                     /gene="citT"
                     /locus_tag="ACHLAM_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO7855280.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6988..7794
                     /gene="rna"
                     /locus_tag="ACHLAM_003823"
     CDS             6988..7794
                     /gene="rna"
                     /locus_tag="ACHLAM_003823"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO7855281.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETATKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISVKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8024..8434
                     /gene="rnk"
                     /locus_tag="ACHLAM_003824"
     CDS             8024..8434
                     /gene="rnk"
                     /locus_tag="ACHLAM_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO7855282.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8577..8666
                     /gene="yldA"
                     /locus_tag="ACHLAM_003825"
     CDS             8577..8666
                     /gene="yldA"
                     /locus_tag="ACHLAM_003825"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO7855283.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8791..9219
                     /gene="uspG"
                     /locus_tag="ACHLAM_003826"
     CDS             8791..9219
                     /gene="uspG"
                     /locus_tag="ACHLAM_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO7855284.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9340..10905)
                     /gene="ahpF"
                     /locus_tag="ACHLAM_003827"
     CDS             complement(9340..10905)
                     /gene="ahpF"
                     /locus_tag="ACHLAM_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO7855285.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11076..11639)
                     /gene="ahpC"
                     /locus_tag="ACHLAM_003828"
     CDS             complement(11076..11639)
                     /gene="ahpC"
                     /locus_tag="ACHLAM_003828"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO7855286.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12011..12757
                     /gene="dsbG"
                     /locus_tag="ACHLAM_003829"
     CDS             12011..12757
                     /gene="dsbG"
                     /locus_tag="ACHLAM_003829"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO7855287.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKMLNIIMGNK"
     gene            12962..>13862
                     /gene="ybdO"
                     /locus_tag="ACHLAM_003830"
     CDS             12962..>13862
                     /gene="ybdO"
                     /locus_tag="ACHLAM_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7855288.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FINRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
BASE COUNT         3377 a         3586 c         3409 g         3490 t
ORIGIN      
        1 agcgaaagga gaaaaagata tacctcgatc gccccccttt ctcccaagtg aaaataaaag
       61 gttatcagtt tgcaacatcg aacaacattt gttgcaaatc gataacaaca tgcaccttca
      121 ggatactatt tattatgttc ggcaatgata ttttcacccg tgtaaaacgt tcagaaaata
      181 aaaaaatggc ggaaatcgcc caattcctgc atgaaaatga tttgagcgtt gacaccacag
      241 tcgaagtatt tattaccgta acccgcgatg aaaagcttat cgcgtgcggt ggaattgccg
      301 gaaatattat taaatgcgtt gctatcagtg aatccgttcg cggtgaagga ctggcgctga
      361 cattagccac cgagctaatt aacctcgcct atgagcggca cagcacgcat ctatttattt
      421 ataccaaaac cgaatacgag gcgctgttcc gccagtgcgg tttttccacg ctgaccagcg
      481 tacccggcgt gatggtgctg atggaaaaca gcgccacgcg actgaaacgc tatgccgaat
      541 cgctgaaaaa attttgtcat ccagggaaca agattggctg cattgtgatg aacgccaatc
      601 cctttacgaa tggtcaccgt tatctgattc aacaggctgc ggcacagtgc gactggttgc
      661 atctgttttt agtcaaagaa gattcttcac gcttccccta tgaagaccga ctggatctgg
      721 tgttaaaagg caccgccgat attccacgcc tgactgtgca tcgcggctcc gaatacatca
      781 tctcccgcgc tacgttccct tgctacttca ttaaagaaca gagcgtcatt aaccattgtt
      841 acaccgaaat tgatctgaag attttccgtc agtacctcgc tcccgcgctg ggtgtaactc
      901 accgctttgt cggtactgaa cccttttgtc gcgttactgc ccagtacaac caggatatgc
      961 gctactggct ggaaacaccg actatctccg caccgcccat cgaactggtt gaaattgagc
     1021 ggctgcgtta ccaggagatg ccgatatccg cttcccgggt acgtcaactg ctggcgaaaa
     1081 acgatctcac ggctatcgcg ccgctggtcc ctgcagtcac gctgcattat ttgcagaacc
     1141 tgcttgagca ctcccgccag gacgcggcag ctcgtcaaaa gacccccgca tgagaaacag
     1201 gtgaaaaatg aaaataaacc agcccgccgt tgcaggcacc cttgagtctg gggatgtgat
     1261 gatacgcatc gccccactcg atacgcagga tatcgacctg caaatcaaca gcagcgttga
     1321 gaaacagttt ggcgatgcaa ttcgcaccac cattctggac gttctcgccc gctacaacgt
     1381 gcgcggcgta cagctgaatg tcgatgacaa aggcgcactg gactgcattt tacgtgcacg
     1441 actggaagcc ctgctggcac gcgccagcgg tatcccggct ctgccatggg aggattgcca
     1501 atgatttccg cttcgctgca acaacgtaaa actcgcaccc gccgcagtat gttgtttgtg
     1561 cctggtgcta atgccgcgat ggtcagcaac tcctttatct acccggctga tgccctgatg
     1621 tttgacctcg aagactccgt agcattgcgt gaaaaagaca ccgcccgccg catggtttac
     1681 cacgcgctgc aacatccgct ttatcgcgat attgaaacca ttgtgcgtgt aaacgcgctg
     1741 gattccgaat ggggtgttaa cgacctggaa gctgtcgttc gcggtggtgc ggacgttgtg
     1801 cgtctgccga aaaccgatac cgctcaggat gttctggata tcgaaaaaga gatcctgcgt
     1861 atcgaaaaag cctgtggtcg tgaacccggc agtaccgacc tgctggcggc gattgaatct
     1921 ccgctgggca ttacccgcgc ggtagaaatc gctcacgctt ccgagcgttt gatcggtatc
     1981 gccctcggtg cagaagacta tgtgcgcaac ctgcgtacag aacgctcccc ggaaggaact
     2041 gaactgctgt tcgctcgctg ctccattttg caggccgcgc gctctgcggg tattcaggcg
     2101 ttcgataccg tctattccga cgctaacaac gaagccggat tcctgcaaga agccgcccac
     2161 atcaaacagc tgggctttga cggcaaatcg ctgatcaacc cgcgtcagat tgatctgctg
     2221 cacaacctct acgcaccgac ccagaaagaa gtggatcacg cccgccgcgt cgtagaagcc
     2281 gctgaagccg ccgctcgcga aggcctcggc gtggtttccc tgaacggcaa gatggtggac
     2341 ggtccggtta tcgatcgcgc ccgtctggtg ctctcccgtg cagaactttc cggcatccgc
     2401 gaagaataag gcaatcaaaa tgacgcagaa aattgaacaa tctcaacgac aagaacgggt
     2461 agcggcctgg aatcgtcgcg ctgaatgcga tcttgccgct ttccagaact cgccaaagca
     2521 aacctaccag gctgaaaaag cgcgcgatcg caaactgtgc gccaacctgg aagaagcgat
     2581 tcgtcgctct ggtttacagg acggcatgac ggtttccttc catcacgctt tccgtggcgg
     2641 tgacctgacc gtcaatatgg tgatggacgt catcgcgaag atgggcttta aaaacctgac
     2701 tctggcctcc agctccctga gtgattgcca tgcgccgctg gtagaacaca ttcgccaggg
     2761 cgtggttacc cgcatttata cctctggcct gcgcggtcca ctggcggaag agatctcccg
     2821 tggtctgctg gcggaaccgg tgcaaatcca ctctcacggc ggacgtgtgc atctggtaca
     2881 gagcggcgaa ctgaatatcg atgtggcttt cctcggcgtc ccgtcctgtg atgaattcgg
     2941 taatgccaac ggctactccg gtaaagcctg ctgcggctcc ctcggctatg caatggttga
     3001 tgccgacaac gcaaaacagg tcgtgatgct taccgaagaa ctgctgcctt atccgcataa
     3061 tccggcaagc attgagcaag atcaggttga tctgatcgtc aaagttgatc gcgttggcga
     3121 tgctgcaaaa atcggcgctg gtgctacccg tatgaccact aacccgcgcg aactgcttat
     3181 tgcccgtagc gctgcggatg tgattgttaa ctctggctac ttcaaagaag gtttctccat
     3241 gcagaccggc accggcggcg cttcgctggc ggtaacccgt ttcctggaag acaaaatgcg
     3301 tagccgcgat attcgcgccg acttcgccct cggcggtatt accgcaacga tggtttatct
     3361 gcacgaaaaa ggtttgatcc gcaaactgct ggatgtgcag agctttgaca gccatgctgc
     3421 gcaatcgctg gcccgtaacc ccaatcacat cgaaatcagc gccaaccagt acgctaactg
     3481 ggggtcgaaa ggcgcagcgg ttgatcgtct cgacgtggtg gtactgagcg cgctggaaat
     3541 tgacacccag ttcaacgtta acgtgctgac cggctctgac ggcgtactgc gtggtgcttc
     3601 cggtggtcac tgcgataccg cgattgcttc tgcgctttcc atcatcgtcg cgccgctggt
     3661 acgcggtcgt attccgacgc tggtggataa cgtgctgacc tgcatcaccc cgggctccag
     3721 tgtcgatatt ctggtcacag accacggcat cgctgttaac ccggcacgtc cggagctggc
     3781 agaacgtctg caggaagcgg gcattaaagt ggtttccatt gagtggctgc gtgaacgtgc
     3841 gcgtctgctg accggtgaac cacagccgat tgaattcacc gaccgcgtcg ttgccgttgt
     3901 gcgttaccgc gatggctcgg tgatcgatgt tgtgcatcag gtgaaggaat aagccatgca
     3961 cctgcttcct gaactcgcca gccaccatgc ggtgtcaatt cccgagctgc tcgtcagccg
     4021 ggatgaaagg caagcacggc aacacgtctg gctcaagcgc catcctgttc cactggtctc
     4081 ctttaccgtg gttgcgcctg ggccgattaa agacagcgag gtcacacgcc gaatttttaa
     4141 tcatggcgtg acagccttgc gtgccttagc cgcaaaactg ggctggcaaa ttcaggagca
     4201 ggctgcactg gtttccgcca gcgggccgga gggcatgttg agcattgccg ccccagctcg
     4261 cgacctcaag ctcgccacca ttgagcttga acatagtcat cctctcgggc ggttatggga
     4321 tatcgatgtc ctgacacccg aaggcgaaat tctctcccgc cgcgactatt cactgccgcc
     4381 tcgccgctgc ctgttgtgcg agcaaagcgc agctgtttgc gcgcgtggaa aaacccatca
     4441 actgaccgat ttactcaacc gcatggaggc gctgctgaac gatgtcgatg cctgcaacgt
     4501 caactaaaaa cacaaagctt gcgacgtcat taatcgatga gtacgccctg ctgggctggc
     4561 gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat cgcattaact
     4621 gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg gcgattcagg
     4681 gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca ccggaagcgg
     4741 tactcaacgg attacgccca attggtatgg cttgcgaagg tgatatgttc cgcgccactg
     4801 cgggcgtaaa cacgcataaa ggaagcattt tttctttagg gctactgtgt gcggcaattg
     4861 gtcgtttgct tcagctcaac cagccggtaa cgccaacaac cgtttgttct acggcggcaa
     4921 gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga ctgacggcag
     4981 gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc gaagcgggtt
     5041 atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat caggggttag
     5101 atcctgagct ggcattgctc gataccttgc tcctactgat ggctaccaac ggcgatacca
     5161 acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag gcgcaaacat
     5221 tattgcaaaa agggggcatt cgaacccccg ccgatctcga ttatctccgg cagttcgaca
     5281 gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg atccttacct
     5341 ggtttttagc acagatttaa ttatttaagc acttgataaa tttggaaata ttaattttcg
     5401 gagaacccgt atgtctttag caaaagataa tatatggaaa ttattggcgc ccctggtggt
     5461 gatgggtgtc atgtttctta tccctgtacc tgacggtatg ccgccgcagg cgtggcatta
     5521 ctttgcagtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc cggcaacggc
     5581 gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc tctttgatgc
     5641 caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat ggggtctggc
     5701 tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg cattagggta
     5761 tgaagtttcc gggttaggcc gtcgtattgc ccttttcctg gtgaaattca tgggcaaacg
     5821 cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac cgtttacacc
     5881 gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc tgccgccgtt
     5941 gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt tgatgtggat
     6001 gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg caccaaacgt
     6061 gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt tgcagtggtt
     6121 cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc tttcctacgt
     6181 gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg gtgatgaact
     6241 aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg tattgctcag
     6301 cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg gtctgctggc
     6361 agtttcgcta atgctggccc tgcacgttgt gccgtggaaa gacattaccc gctataacag
     6421 cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg gcctgactcg
     6481 ttctggtttt attgactggt tcgccaatac catgagtacg cacctggaag gattctcacc
     6541 aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc tgtttgccag
     6601 cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta aaggtattcc
     6661 gggcgtacca atggaacaac tgtgtatcct gctggtgcta tctatcggta tcatgggctg
     6721 tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct atgtgaaatc
     6781 aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta tgttgctgtt
     6841 ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc tgaataaatc
     6901 atcacgcccg ccaggtaaac ccacctggcg ggctttttta tgattaaata gataatattc
     6961 agtgtatgta tgagttccac acccattatg aaagcattct ggcgtaacgc cgcgttgctc
     7021 gcggtttctc tgcttccctt ctcttctgcc aacgccgtgg cgttgcaggc aaaacagtat
     7081 ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg ccagagtcaa
     7141 cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac gaccaacaaa
     7201 gctgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt tgctgcccgt
     7261 ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat cccgaatcta
     7321 ccagaagcgc gcgccagccg aatgtgttca tcgccggaaa ccgggttatc actggaaacg
     7381 gccactaaac taagtgaagt catgccagga gctggcggac gttcctgcct ggaacgctac
     7441 gaatatgcca aacacggtgc ctgctttggt tttgatccag acgcatactt cggcacgatg
     7501 gtacgcctga atcaagaaat taaagaaagc gaagccggaa aattccttgc ggataactac
     7561 ggtaaaacag tgagccgccg tgactttgat gccgcctttg ccaaaagctg gggaaaagag
     7621 aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac tgaaattcag
     7681 atctcggtca aagctgacgc catcaacgct ccgctttctg ccaactcatt cttgccacaa
     7741 ccacacccag gcaactgtgg caaaaccttt gtgattgata aagcaggtta ttaatcctct
     7801 gatttacaaa acacgctaat tcgtcttcca taaaccatga cgattaagat ttatacataa
     7861 tgcgctacat tgaggaattc cctgaatgtg acgcaaatca cttcaaagag tgagcaacag
     7921 tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt cttttgggta
     7981 tgtttatctg cgacactcgc agtaccgacg acatggagta aaaatgtcca gaccaactat
     8041 catcattaac gacctggatg ccgaacgcat cgatattctg ctggagcaac ccgcctatgc
     8101 tggtttgcca atcgccgacg cgttaaacgc agagttggat cgcgcccaaa tgtgttcgcc
     8161 agaagagatg ccacacgacg tggtgacaat gaacagccgg gttaaattcc gcaatcttag
     8221 cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg acagcaatac
     8281 tcagctttcc gttatggctc cggtaggtgc cgcactgctg gggctgcgcg ttggcgattc
     8341 cattcactgg gaacttccgg gcggcgttgc aacccacctt gaagtgctgg aactcgaata
     8401 ccagccagaa gctgctggcg actacctgct ttaatcccat ctgcacaagc gataactcgc
     8461 tttgcccaga ggatgcactc gcatcctctt tccgctcaaa tatcaaccag atttatcatt
     8521 ttgctcttaa gcgcagcatt ctaatctgaa ctggctattc atctgcggaa cctcatatga
     8581 gcgaagctct cggtatcact gcattttata tattcatagc agcaattatt gttgctgcag
     8641 tgctatacct tgaacaacgc tggtagcgcc tggtttttat ttattttcca gctcaatagc
     8701 aaattcagat caaatcagcc tcgcaaatta tagattcgta accaataatg tgctgttatg
     8761 tatacatgca aaaacaagga gagaatgctt atgtacaaaa caatcatcat gccagtggat
     8821 gttttcgaaa tggaattgag cgacaaagcg attcgccacg ctgaattcct cgcccaggat
     8881 gacggagtta ttcatctact tcacgtacta ccggggtcag ccagcctgag cctgcaccgt
     8941 tttgccgctg atgtacgtcg ttttgaagag catctgcaac atgaagcaga agaacgtctg
     9001 caaacgatgg tcagccactt caccatcgat ccttcccgca ttaagcaaca tgtccgtttt
     9061 ggtagtgtgc gggatgaagt taacgagtta gcgaaagaac ttgatgcaga tgtagtagtg
     9121 attggttcgc gtaacccatc aatttcaact catctgttgg gttctaacgc ctcgagcgta
     9181 atccgccacg ccaatttgcc ggtgctggtt gtgcgttaat tgccagaata agtctcccgc
     9241 cctgcccgga gccatccctg cagggctttt taatgcaata aaaaagccgc caggtttgac
     9301 cccggcggct aagcaattac aggtgaatct tacttcttct tatgcagttt tggtgcgaat
     9361 caggtagtca aaagcactca gagaggcttt ggcaccttcg ccagtggcga tgatgatctg
     9421 cttgtacgga accgtcgtac agtcacccgc tgcgaacacg cctttgacgt tggtttcgca
     9481 tttcgcatca atgataatct cgcccatgcg attacgttcg actgcgcctt ccagccagtt
     9541 ggtgttcggc agcagaccaa tctgcacgaa aataccggcc agttcgatgt tgtgaatatc
     9601 gccgctgaca cgatctcgat attccagacc tacgactttg ctaccgtcgc ctttcacttc
     9661 cgtggtttgc gcattcagaa taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag
     9721 aacctggtcg gctttcattt ctggcgcaaa ttccagcagc gttacgtgct caacgatacc
     9781 cgccaggtca attgccgctt ccacgccgga gttaccgccg ccgataaccg ctacgcgttt
     9841 acctttaaac agcgggccgt cgcagtgcgg gcagtaggtc acgcctttgg tgcgatactg
     9901 atcttcgccc ggaacgttca tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg
     9961 tgctttcagt accgcgccag aagctgtttc aatctgatgc aggccacctt caaccgctgc
    10021 cgggatcagt ttgctggcgc tctggctgtc gatcacatca acgtcgtatt catcaacgtg
    10081 aactttcagc gctcctgcca gtttctggcc ttcggtcttc ggtacagaaa tgtagttttc
    10141 gatatcaacg gtatcgagga tctgaccacc aaaacgttcg cccatcagac cggtacggat
    10201 gcctttacgt gcggagtaaa ttgctgccgc tgcacccgcc gggccggaac cgacgattaa
    10261 tacgtcataa gcatcacgct tgttcagctc ttctgccgca cgtttttccg caccagtatc
    10321 aattttggca acgatttcag tcaacgtcat gcggccctga ccaaactctt tcccgtttac
    10381 gaacactgcc ggaacgccca tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc
    10441 gtcaattgca gtgtgcttga tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac
    10501 cacgtccggg cagttgtggc aagagagcga gtaataggtt tcgaattcaa aatcaccgtc
    10561 aatatggcga atctgctcca gcagagactg cgcttctttc gacggatgac caccggtcca
    10621 cagcaacgcc agtaccagcg aggtgaactc gtggcccagc ggggagcctg caaaacgtgg
    10681 cccctggtta gaacctgggt tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt
    10741 atcttcttta aaggtgactt tgtctgacag ttctgcgatt tcagccaaca gttccttgat
    10801 ttctgccgat ttagcgctgt catccagcgt ggcaattaac tcaacaggct tggtcaattt
    10861 ctcaaggtaa gccttgagtt gagttttcat atttgtgtcg agcatgttta tctcctgggc
    10921 tttaaacatc atcatgcaag cggcctctgc ggctacctgg atgcagcttg catcatggtg
    10981 cggggaagaa aatcgggtgc cgcagcaccc gaagaattag gtgaatttcc gaagtatttc
    11041 gcgccggagt tagtagcgcg aaagacaaag gaaatttaga ttttaccaac caggtccaga
    11101 gacggagcca gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga
    11161 gaagctacgt actgtgctgc tttgatttta cgcagcaggt cagacgcgtc acggccaatg
    11221 ccttcagcgg taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca
    11281 cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc
    11341 gggtcgccga tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct
    11401 ttgtgggtga agtgagtatc ggtagatact gcgtatacgt ctacgcccag tttctgcaat
    11461 tcttcgtaat ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc
    11521 gggtagaaga agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat
    11581 tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc
    11641 tatacttcct ccgtgttttc gatgagatgt aaggtaacca atttttgccg attcggctaa
    11701 tgcgtttcca ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg
    11761 aactttgcac cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta
    11821 atctgaacta ccctttggta tcttcagcgc ctgcctgcag gctgaggtgt tgcccttaca
    11881 aatgcaacaa cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa
    11941 gctcttacct atgacagtga taggttatgc cttttactcg acttttgcac tgactgaaaa
    12001 ggacaaatta atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc
    12061 agaggaactt cctgccccag taaaagcgat tgaaaaacag ggtattacaa tcatcaaaac
    12121 gttcgatgcc cctggaggaa tgaaaggtta tctcggtaag tatcaggata tgggcgtcac
    12181 catctacctg actccagatg gtaagcacgc tatctctggt tacatgtaca acgaaaaagg
    12241 tgaaaacctg agtaacacgc ttatcgaaaa agaaatttac gcacccgccg gacgtgaaat
    12301 gtggcaacgg atggaacaat cccactggct cctcgacggt aaaaaagatg cgccggtcat
    12361 tgtctacgtc ttcgccgatc cgttctgccc atattgtaaa caattctggc agcaggctcg
    12421 tccgtgggta gattctggca aagtgcaatt aagaacattg ttggttgggg ttatcaagcc
    12481 agaaagcccg gcgacagcag cggcaattct tgcctccaaa gatcccgcaa aaacctggca
    12541 acaatatgaa gcctctggtg gcaagcttaa gctaagcgtg cctgcaaacg taagcacaga
    12601 gcaaatgaaa gtgttaagcg acaacgagaa actcatggac gatctggggg caaatgtcac
    12661 accagctatc tattacatga gtaaggaaaa taccctgcaa caagccgtcg gattacccga
    12721 tcagaaaatg cttaatatca ttatggggaa taaataaaag ataattcatg gatggttgaa
    12781 atatatcctt gcttttcaac catcctaaaa acgcatgaat tgctcgtaac ccacctgaca
    12841 gctatgctat tttagaaaat cttaatccaa agttaagcag aaaaacataa tacatataaa
    12901 tacaaaaaca ttattgatca agtgaatgat ttatggaaga gacaatacaa cggagtatgt
    12961 tatggctaat ctttatgatc taaaaaaatt tgacctcaat ttactggtca tatttgaatg
    13021 tatttatcaa catctcagca ttagtaaagc ggcagaatca ttatatataa cgccctctgc
    13081 tgttagtcag tcgttacaac gtttacgtgc gcaatttaat gacccgttat tcattcgctc
    13141 agggaaaggt atcgcgccga ctacaacagg gttaaacctg catcaccatc ttgagaaaaa
    13201 tctcagaggc cttgaacaaa cgatcaatat cgttaataag tctgaactaa agaaaaattt
    13261 tattatctac ggtcctcagc ttatttcttg tactaataac aacatgctaa tccgctgtct
    13321 tagacaagat acttcaatcg atatagaatg ccatgacatc ctgctttcag ctgaaaatgc
    13381 cgaagaactg ttggttcaac gcaaaactga cctggcaatt acactgcaac cagtaattag
    13441 tcgctctgtc atctgcatgc cattacacac tattcgcaat tcccttatat gtagcaacaa
    13501 acatcctcgt attactgata ccagtaacta tgaacaaatt atcgccgaag aattcaccct
    13561 gttgatatct aaatcagcag ggattgacga aattcaaatg gacatagatg aacgttttat
    13621 aaaccgtaaa gtttcatttc gcagttcttc tttgttgact ataattaata gcatttctgt
    13681 tactgactta ttaggcattg tgcccaccga actgtacgat ttacatcgtg attttttgaa
    13741 gttaaaagaa ataaaactag aacaacctct tcctgcggtt aaactttata tttcctataa
    13801 caaggcttcg ttgaacaacc ttgtcttctc ccgattcata gatcgtttaa atgatagctt
    13861 ct
//
LOCUS       DAZGAP010000147        13722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGAP010000147 DAZGAP010000000
VERSION     DAZGAP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.148"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(294..8681)
                     /locus_tag="ACHLAM_003831"
     CDS             complement(294..8681)
                     /locus_tag="ACHLAM_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO7855289.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQM
                     SPGAVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAG
                     MDVSPWEGVTDAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAP
                     QPEPVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNAPMAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAPENAAGRQ
                     GETLEGEIVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVFTDRESTVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAKV
                     WFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKDMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAH
                     YEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRRI
                     FKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAAR
                     AFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTL
                     KTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGAGASSQYSRSGKPDPFKADAYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPV
                     LRALGAPNKELVMPAHVVHKATRPELREHYVSVETMADLPQLLADPVAVMTSKTEHDS
                     LVTLIEAKDRRGAPVIVAIHMRAKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNK
                     EKAADWLLSAGLQLPGANTINGSSGFKVLHPEDIRKGAYYSRTSSLTPEETIASRFVR
                     QMQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKL
                     LANYKISQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGK
                     QAQYDRLAGIVDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLP
                     RTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNP
                     DKDYWQVFTDDRPDTMRTIAERKDQETGETRREVVERPVPMAMMADRYFTTKKNGKTY
                     YIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTA
                     VMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWK
                     EFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVE
                     NALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTAN
                     LVRTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWY
                     DKVPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRA
                     AGNVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENM
                     PFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKYWIDYISGG
                     TGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAE
                     LKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMV
                     KSVERDMKIAVDRFNREYNKKVGVE"
     gene            complement(8767..10029)
                     /locus_tag="ACHLAM_003832"
     CDS             complement(8767..10029)
                     /locus_tag="ACHLAM_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855290.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLAKLQEVASNVAADAIKNGRDAQAEVDEFYAANGLQ
                     PHQQKVQQRKTQQLLQKWQTWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
     gene            complement(10040..10291)
                     /locus_tag="ACHLAM_003833"
     CDS             complement(10040..10291)
                     /locus_tag="ACHLAM_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO7855291.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(10301..10747)
                     /locus_tag="ACHLAM_003834"
     CDS             complement(10301..10747)
                     /locus_tag="ACHLAM_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855292.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAV"
     gene            complement(10750..11406)
                     /locus_tag="ACHLAM_003835"
     CDS             complement(10750..11406)
                     /locus_tag="ACHLAM_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855293.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(11500..11853)
                     /locus_tag="ACHLAM_003836"
     CDS             complement(11500..11853)
                     /locus_tag="ACHLAM_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855294.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACLKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(11989..13194)
                     /locus_tag="ACHLAM_003837"
     CDS             complement(11989..13194)
                     /locus_tag="ACHLAM_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7855295.1"
                     /translation="MKKASKLLLALMPALSLSVFSAGVYAADASGINGTSYYGVTVDQ
                     LKGHEYKTPIDFWRDLGFSGKGYLDGVVVNADTRKPESVNIQNTAGKYLKYSLYDVGK
                     SGLTNTELLAPRSEIASYYDYANEVAYFVNTKTNQILFSADGEGLVTTVGADGKAYSR
                     HIPEISEYIYEIKIKSVIPGGTIDSTLSAADVTDIHARLSSLGTGKTQVGGGATAGGE
                     NATAVGYNAIANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAATYGQLER
                     YRIAEENARIQGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRKRADAGIA
                     GAMAMTAIPVVEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASWDTQNGSG
                     VAAGMSVGW"
     gene            complement(13546..13686)
                     /locus_tag="ACHLAM_003838"
     CDS             complement(13546..13686)
                     /locus_tag="ACHLAM_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7855296.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLYGSLCEFRLNLWGAEFAAFLQC
                     KQ"
BASE COUNT         3039 a         4053 c         3064 g         3566 t
ORIGIN      
        1 tgattaattg cggctttatt caggatattc cgaaaataac cccaaaatat taatttaatg
       61 ttttaggatt tgtcttacat tattcttggt gtagtcactc agaaccacga ctacaccacc
      121 ggaacaggca tcagaaaaaa cttactctca tcaggcattc tgtttcacat ctgcttacac
      181 tgtaatttct gatcaatatc acaacattat ccgaatttaa ctgttcagtt catctacaat
      241 atttccctgc gtgactttat atcagccccg cacttccgta cggagcatgt tctttactcc
      301 actcccactt ttttgttgta ctcgcggtta aaccgatcca cggcaatttt catatcccgc
      361 tctaccgatt tcaccatcgc cgactgttgc gccagactga gagtactgtc ggcgtaaatg
      421 gcatcacgct gtttacgcaa atccttcagt ctcttccggg tatcctgcat aagcccgttc
      481 atcgacaatt ttccgttgtt ctcgtcaatg aacgccgttc tttctgcacc ggtcagactc
      541 ttcagctctg cgtggtactg cgcaacctct gtcatccggt cgtacatctt ctgctggtca
      601 gcatacggca tcacctcacc tgaaattttc cccaggaaag gcacctgctg ctccggtata
      661 tcaataccat tcagcgattt caccgccgca tccgtggttt tggaaatgaa gcgccctgta
      721 ccaccagaga tatagtcaat ccagtatttc agtgattccg gcgtgacatc caccgcgccg
      781 ggacggtact ggctgccacc tgagaacgca ttcagccagg atgcaaacgc cttgtacgct
      841 tctggcgttg aacgtcttcc cagctggctg tcaggttttg gtgtaccaaa cggcatgttc
      901 tcctggtaaa tctgcgcccc catgaagttt tcattcatgg caaggttcgc aaacggacgc
      961 agaatggtcg gcgctgcatt tttcagcaat gccccggaca gtgtttccga cgtctcactg
     1021 ccaatcgggc tgaatgcacc aagcacacca ccaacaacat taccggcagc acgggacgcc
     1081 gtcaggtcac ccgccgccac accttcagcg gtatgcccga gcaggaagaa aacgttgtac
     1141 ccgtaaggca gaggaatact ccagtactct ccggccttgc ccccgaacac cgatttcata
     1201 atgacgaggt tacgctcttt cacatgagac ggcaccttgt cataccagtt aaccccgtca
     1261 tcatcctcac ccgccacact gcggttaagc gagccaagca gataacctgc tcccacagcg
     1321 gcaagcgcga ttttctgcgg aacattcaga ttcttccagc gaaggcgctc cagtaaaggt
     1381 ccctcaccat taagatgtcc gagcgttctc accagatttg ccgttccctg aatactggcg
     1441 ttggcgaaca tgtacagcga gttcatcagc gccccctgct caccacgacg gttaaagttc
     1501 accgtcatgt ttttggcaag agacgccgcc tgctggcgtg acaaaccggc atcacgggcg
     1561 tgtttataag ctgacagacg cagtgcgttt tcaaccgcgc cgttggcatc ctcgacaagg
     1621 ttaaggaacg aattccatgc accgatactc tggcctttcc atcctccctt cgccagcgaa
     1681 acaaggcgat ccatttcctt ctgctggcct tcaaggtcac ccatgttaaa ccaaccggtt
     1741 ttacctccgt cctcaacaaa ctctttccac accttctgcc actgtgcacc tttgcccgtg
     1801 agggttttac cacgcagact ggcgtatacg gctgacatgg cagaacggct gtctttgact
     1861 acagccaggg cggataagtt atccagccct ttcagtttac cgtcgctcct tccctgctca
     1921 gccttcaggt tcatcaccgc cgtctgcacg tcacggatga agttactgac caggaattcc
     1981 gggttatacg acgtgttcac cgttgccagg aagcggttaa ctttccccag cgtacggatt
     2041 acggcgttac tggtttccgg tcccatattc ttcatcgcac gcatcaggcg cgggtcatgg
     2101 agtttgatgt agtacgtttt gccgttcttt ttggtggtga agtagcggtc tgccatcatt
     2161 gccatcggta cagggcgttc gacaacttcg cgacgggttt caccagtttc ctggtccttg
     2221 cgctctgcaa tcgtccgcat ggtatccggt ctgtcatcgg tgaatacctg ccagtaatcc
     2281 ttgtcgggat tatcctgcac cagtttcagg aaggcgttac ccacttcgtt tttgcgatgg
     2341 cggatcagcg attcgctcag gtcctgtatc gcctgagtgg acggagactg tgcacgggat
     2401 gcacgcccca tggcctgcct gctttcacgt ccgccgatgg tgaagccctt acctgtacgg
     2461 ggaagtgaca ccacaccgtc aacatcctga cctttcaggg gaacgtagta acggtaggcg
     2521 ttctgccagg catccaccac accgttctct tcaagtccgg cctcacggat aagctcacgg
     2581 cgacgggcca gcatatcgtc aacaatccct gccagacggt catactgtgc ctgtttgccg
     2641 ctgttacgca cacgctgcat gatttccgcc gcctccgcgt tggtcatccc cgaaccgccg
     2701 tccggcattt tagggttgat tttcgcaata tgtgcgttac gttccggcgc gtgacgggcg
     2761 tagaggtact catccagatc ggcctgagag attttgtagt tcgccagcaa tttggccagc
     2821 ggctgaacgt agcgctcctt cattacgttc aggtcgtttt ccgccttccc gtggaagagt
     2881 tcttcagcca tataggcgtt attgctgtca tctatttttc cgccagtttt acggatattc
     2941 tcctgcaccg ccttcagcac ctggaattta tcctgcatct ggcgaacaaa acgcgatgca
     3001 atggtctctt ccggtgtcag actgctggtg cgggagtaat acgccccctt gcgaatatct
     3061 tcaggatgta gtactttaaa tccagaagag ccgttgatgg tgttcgcccc gggcaattgg
     3121 agcccggctg aaagaagcca gtcagcggct ttttctttat tcagatacag caaggaagta
     3181 tccaactgtt cctgcaaagc gatcctgttg tcctttccgt agacactggc aatcttatta
     3241 acctcagcta ccgccccttt agcacgcatg tgaattgcaa caatgacagg tgcacctctt
     3301 ctgtccttag cctcaatcaa agttaccagt gagtcatgtt ccgtctttga tgtcatcacc
     3361 gcaaccgggt cggcaagcaa ttgcggtaag tcagccatcg tttcaacaga aacatagtgc
     3421 tcacgtaatt ctggtctggt ggccttgtga accacatgag ctggcataac cagctcttta
     3481 tttggtgcgc caagtgctct taacactggc ggagtgtcac caacccgcac ctctccacct
     3541 cgtggaaccg attgtaatcc ggcaatgcga cgcacttctg aagcaaactg ctttgcatca
     3601 taggcgtcag ccttaaacgg atccggctta ccactacggg agtactgaga cgatgcgccc
     3661 gcgccatcct cacgcggcgt gtaaccttcc cgcacacgct ggcctaacgt acggatggtc
     3721 tcgcgaacaa gtctgatatc gttcagttcc gtcggcttca gtaaccccgt acgacgcagt
     3781 acccctttga ccagggcaac aacacgctcc catgccgcca cgaatttatt cggctgtttc
     3841 tccgccatat gtgccagaaa ttcacccgcc tgtacttccg gtgattcctt accataggac
     3901 gcatcaacct tacgccaggc ttcacggatg gtggcgttat cactgtcgcg ggttttcagc
     3961 acggttttga taatcgtctg atattccgct ggcgtgacaa catgctccat ggcatggtgg
     4021 ataatctcgt gacgcagctt ctcgcgtacg gtccgcccgt cagggatgtt atccgccacc
     4081 aggactattt ctcgtttatc cggacgatag aatgcgtgca ccctgccgta accatcgaac
     4141 gattcgcccg ccagcgcttc agcctctttc tgtgacttca ccacacggac cttcaggtca
     4201 ctgtccttaa tgccgctcat cacgccacgg gcaaccgctt caacctgcgg gaccgggctg
     4261 cctttggctt ccgccgcact acggttaaca tccgaaatga gattgccttc aggtgtgcgg
     4321 gttacgccct tacgggaata aaacgcaacg cccttgtccg tctcacgggt tttcagcgta
     4381 ctgaacaggt gatcgaatgc atcacgaata ccgccatcca gttccgcatt cgtcggataa
     4441 gcccaggtgt tatctgtgtt gtgctcaggt gccttacgga tattgaccag ataatcattc
     4501 tccacgccag ccatacgcgc cttatcctga acataacgct caaaggcacg cgccgccatt
     4561 tcaacatccg ttgaccagta cggttttgag cgcacctcat caagaagcgc tgaacgacgc
     4621 ggcatgtcac tgtttttaat ggcctgaatc acacctttaa aagcgtcgta aacctcctga
     4681 cgtaccggat attcagcatc aacatacctg ccgtctttaa atatgcgcct gacacgctgt
     4741 gcttccgtca taaagtcgcc acctgacgta attttcccgt cattggaaac gtcataacga
     4801 ccaaaataat tatccagaga atggaaccat tcgtgcgcca gcgcacccgg tccgttacct
     4861 tttgtcaggt tgattgccac ctcacccggc tcatagtgtg ccgccgcctt acccttacca
     4921 cgggcaccaa atgccaggcc aagacgaccg ttcagggaaa gcgcttttgt cggtacattc
     4981 agtacttccg caaggtcatg cagcgagtca taagcccggt tcaaatcagc ctgacgacgc
     5041 ggaccttcca cgtagttacc aaactgcaca ccacggaaac caaacgcatc actgaactgc
     5101 tccggtgaaa catccccttt acggcgttct ggtccggtac ggtcgcggtt ggtggcgtta
     5161 cgctgctcct cacgcgaaat ctcccgcata tccttcacat gacgaacaag ctcatcacga
     5221 tgtgaatcaa tgtacttacg cgcatcactg gctgacttaa agccacctct cacccgcatt
     5281 ttgtttttac cgtaagcgat aaaaatatcg ccactgcggg tattccggta aacgtcaaag
     5341 cggattttgt catccggtga cggtgctgtt ttttcatcac ctttcgcctg tgatttttcc
     5401 tcctgctctg caaaccagac tttcgccttt gccagtaatt catccctgct ttccgagaaa
     5461 aagaggttac tccccttatt gtccttattg cgcagtgaat aaagtttctg tggcggatcg
     5521 taacgcttcc ctcctgccgc ctgatacaca cccgatacca cccgataagc agaagccctg
     5581 tccatctgtg agggtggcag agtgcgtaac agttgccagg tatccgcgta acgggagggc
     5641 attctgcctt ccatccattc tgcgaggcgt ttcgcgctga ccgttccgtt cagcatttcc
     5701 gatacactgt gtcgtacttt ttttacgctc tccccccagc ctgccgtatt gtgtttcgtc
     5761 ttcgccggaa tatcgctacg atacagcgct atcattgcca gagtgtcagc atcggcacct
     5821 tcgttcgcca gttttgcgta gtccggtttc gggaacagtt tgctcagcgg ctgcgtggca
     5881 taatccctgt cttccagcgt tttacccagt gtttcagcaa gctgtgcata acggtgtttg
     5941 gctgcccctt taatttcctc gccaaagtct tcaatttttt tgcccctgac ttcacctccg
     6001 cgcacggtag attccctgtc agtaaagaca gttgtttcag attcaggtgc gattgtctca
     6061 ggctgcggga tgtcacgatt tcctccggcc tgtcggcgta cttctgcctg ctgtgcgagc
     6121 tcaggcagtg agtcccgtac agtgcgggga agtgattcag gctgaggcat acgaacatta
     6181 cgcgcaatgt caggagccgg tagcccttca cgtaccggtg ctgtcgcgtt ctgcgcatcc
     6241 ggtgacggca gaccacgacg aactatttca ccctcgagtg tttccccctg gcgaccagcg
     6301 gcgttttccg gtgccggagc acgtcctttc tggaaactct ggcccttaac ctcaccggtt
     6361 gtggtaaaac gaccaccacg tccggcctga ttctcatccg gcgtacgcgc cacctctccc
     6421 ggtaacggat atccctgtcc gggatgaata tcgccggggg cgggcagacg cggacgctca
     6481 gttaactcct gtgctgtcgg accggcatca ccctcagcca tctgtgaacg tacaagctcc
     6541 tccgccatcg gcgcgttgcc acgggcaagg cgggcctgta cctccgtatc atcccccgtg
     6601 aaaccacgga atcgcgggtc acgcatgaac gcgggctgct ccatcgggtc agcatcagca
     6661 atacgctctc cgtcagccag ggtgttaatg gtttccgttg ccacatcttc agagaacgca
     6721 ggattatcca tttcaacggc atactgattc tcgcctttct tcactacgga cggtttcagg
     6781 ccggtagcgg cggcattacg gaaaacatca ctccccatgg cgcttttctc atccgtgaaa
     6841 taacggttgt ccgggcgcac cttttcaatg cgttccgctg gtctggacgc accattcccg
     6901 ttaatttcaa tgcggacttc attcgggtcc cggaaccgca ccgtcgggta aatgctcccg
     6961 ttaccatcct ccgcattttc aggctgtgac tgtgcttccg gcgtcacttc ctgttgtggg
     7021 cgaatgactt ctcgtattgc ctgaaactgt tgttcttcag tctcggtgcg ctcctctttc
     7081 tggctgagtc tgcggtattc ctcaagcagt tcagaacgcg gcttcgcctt cagctcattc
     7141 atcacagcct gtcgcttcgc ctgttcgtcc agttcattca acaactggct ggcagcttcc
     7201 cggcgatgag ctgcggatgc gtcaccctct gttgccatat ccgcatcagc atactgttcc
     7261 agaagctgct cgcgattcat gccctgcatg gattcacgat gctgtgccac cggatcaacc
     7321 ggttcgggtt gcggggctgc gtcgtcctgc tgcacggttt cagcatcacg catggctgct
     7381 tcttctgcgg cctgacgtct gccacgatat ccggcaaccg caccgaatgg agcccccatc
     7441 gcagcaccga aaacagtgcc ttcaatccct gcgtcagtca ccccctccca tggcgataca
     7501 tccattccgg cggtctcacg caatgccatg ttttcctgat agcgcgaata ggcgttctgt
     7561 gccccgttaa ttacgccctg ttccgtggca tttctgacaa tgccgctttt aacggtcttc
     7621 gctgtgcctc gtgtcaccag attaaacagt tgtgcgtcac ccagttttgc cgccatggca
     7681 ttcacagcca gcaattcagg atcggttgcc agctgcgcgc gcacctcatc ggcaacacgc
     7741 tcttttgcca gatccatttt ctggcgatca gtaagctgtg catgctgcgg gtcggcgtca
     7801 atggacaaaa acgtctgctg aaatttcggt gactgcgcca gctcagagta atccgcatta
     7861 agaacagcat ctgctgccgc cattgcactc tgcccctgtg cactggctgt ggaatgggtg
     7921 atcaggcccg cctggaataa atccggcatt tttttatcga cagcttctgc tgccagtgcc
     7981 gtggctcttt ccggctgcat ccctgccgcg atgtattttt tctccagccc ggcggtcagc
     8041 atttttcgca gtgtgacatc acccaccttt ctagccacac cgccagcaac catatcaggt
     8101 acaagcgcac caatcaggtt tacacccttc gccagcaaga ccgcagaatc atcatagcct
     8161 tctgttatcg gcgtattcag cgcacgaaca gcacctggcg acatctgttc tgtcagccat
     8221 tcatccgagg ctttggcacc atcactgaca gccttaccgg ttgctttcag acctttaccg
     8281 acagtatcag tgaccacgtt ttttccatca ggcagggtat cgatgacctc atcagccccc
     8341 ctgctgccac cggcaaaaat atcctgaaca gttgcgacac ccggtagccc catacggctg
     8401 aactcattta aaatacgcgc ccctgttttt accgggctct gaatcatcgc atcaccgagt
     8461 ccacgggcca tttcccctgt tccccggacg gactgggcaa cacctttacc cattgttggc
     8521 aatacatcac cgaggctgaa cgatgtgctg tgatcaatgg cattctgcgg gttgctgaaa
     8581 aaatcctgcc agtaattttc tcccggctgc ttaatgttca ggctgctgcg gttctggtta
     8641 cccaattgcg cttcagggcg ctgctcatct gaaaaagcca tacagactcc agtaaaaaaa
     8701 agcccgacac gatggtcggg cgtaagtgaa cgatattaac ggggagcgcg ttactgccgc
     8761 atatctttac ttcatccagc ctttgatctt ctcggcagct gcggaagcgc tataatccag
     8821 cgccttttca tgctctgcct ttttatcccg gaggtgattc tggtaataaa tttccacctc
     8881 cttttcacga ccaggttcca gcaaagcagg cgcattggcg gcagcctctt ttacaaactc
     8941 ctgtttaacc ggatcgcccg ccgcccacgt ctgccacttc tgtaaaagct gttgcgtttt
     9001 ccgttgctga actttctgtt gatgcggctg taaaccattc gcagcataaa attcatccac
     9061 ctcagcctga gcatcacggc cattcttgat cgcatccgcc gctacgttgc tggctacttc
     9121 ctgaagtttc gccagagaaa gtccctgctg cttcggcata aaataaccat aatttttcga
     9181 tatctctgca aatttgctgc gatccctgac ctgagcgata gccttatcaa cgggtatcgc
     9241 cagcacggtt tgatcatcag ggtgcgcact gccgtattct gttacaggtt tatgcgcggt
     9301 ggagccatcg ctgtatgtga gatcaaggcc aataagtacg tacccttcct gctgcgccgg
     9361 tacgatactg ccaatcctgg cctcttttat cgttttctcc ccggttgaat caggcatgcc
     9421 aatacgctgt tgtagttccg gggcaaacac gccggaaagc acatcgagat tttccggggt
     9481 attcagcgaa gcaatcgccc tgtccggctt atcatcaaag attttctgca ggttgttcac
     9541 ggcctgaccc gctttcgcag catagcctac cgttgacatc atcttaagcg gattggcgtc
     9601 cgataattta ccgaacagat gagaagccgc atcatggtca cccgcatcaa tcgcttttcc
     9661 cagcgccgcc atcacaggct gatcgcgcgc cagcatatcg ttatactcaa gccgtcgctg
     9721 gttgtatttc tgcaactgga gtcgttgctg ttccatcccc agcgacgcat tccggtaatt
     9781 ctggttggcg ttaaactccc tttcctgcag tgtgtaattg cggtcatcaa ctttggctct
     9841 gtaatcaaag ttccgctgat caacgtcttt attatgttca aactgagact gcgcaaattc
     9901 aaaatcgcgc tcgttatttt tctgttgctg agcaagctgt gcttctcgca aaccaagctc
     9961 cttacgacgg gtcattgcct ggtcaacagt gctgaatccg gcaagtaacc cctgtgcaaa
    10021 tccgctcatt caccactcct taaaacaaag aaccagcaat gccgccaatt actgcaccag
    10081 caacagcgcc aacaggacca ccaacggatg cgccaatagc cgcccccgta ccaatgcccg
    10141 taccgatatt ctgttttttc tgagctttct gttgagcagc cagttgttta tttgatgcct
    10201 caatctctgt gcgtcgtctg tctgcgtcac ttattccctg taatgcctca cgccgcgact
    10261 gatttgcaat atccagtaaa ccgtatccca tattgccccc ttacactgcc accagttgtc
    10321 cgccaacact cagtttctgt cttgccggtg cagaagcccc cgtcagaata ttcatctggc
    10381 gatcctgttc ggcttcacgg ataccatttt tcgcgccagc aattgccagg gcattacgta
    10441 accccagcgt attactgtcg ggattatccg ggcggcttac cccgtatctc gccatctggt
    10501 tatcctgcgc catctgcgct gtacggagac tggaagtggc aaggccgccc acccgggcaa
    10561 gctgtgcatt catcaggctg ttgttctcgc caaggtcagc cagccttgcc acgcggggca
    10621 aatatctggt tcgccagtcg tcgtattgtt ggcgtgtcag cgctgctgac gtctgccagt
    10681 caccctgtgg acgggcagct ccggtataac cgtatctcgc aagtgtttcg tatttaccgt
    10741 actccataat cacagtctcc agttctgagc ctgatgctga atggcattag cgccggtgcc
    10801 aggtgtttta gcaccgccac ttcctccgct accgccagcc ttatgcatcg catatgcacc
    10861 tgccgcaccc aaaccagcgc caacaagaga ggctcgcccc tgctgtttcg taaacgcagc
    10921 ctgtgcgtcc gatttagctt ttgccagact gctgtctgcc agcgagttaa aactctgtaa
    10981 cgcatccgcc ttctgaccag aaccgagagc tgcaacatcc tgtagcccgg caacatactt
    11041 atctgcctgc gatacctgcc cccgtgtggt tgtgtcaatc tgcccggtta cctgatcact
    11101 ctggttcgca ttcattaccg cgttaaagcg accactggac ggatcaacac cggactgagc
    11161 aagattaccc gccagctcct tccgtgcttc gccaaactgt ttctgataac caagatttgt
    11221 tgtgccagcg atattttcgt actgctgctc actgttcagg tcatcgacct tttccatgaa
    11281 gttatcttct gccggacgga ggattttttg ataatcctgc caccctttcc aggccacttc
    11341 ttcctgcgct atttgcgctg ctgttggttt tactttggta tcaccaccgc ctttacttcc
    11401 acccataatg gccccctgga taacaaaaaa ccctgccgga gcagggtcag aatgtgaatt
    11461 acaatgttgg tttaaacaca atgatgaaaa ctgtagcagt cagaccgcta tcctgaacac
    11521 catgaagcca tcctcatcat ccggcattcg ctcaaagccc agtcgttttc ccagccggat
    11581 aaatccccgc cttgccgtat gaaattcagc ccagcgtccg ccagccagac gggttaatgt
    11641 cttcacctcc ggcagataac gctcaacgct gttactcccc gtacacacgc ccagcaacac
    11701 cagaacataa gggataccat cagcactgag cacagaacgc agcaccagaa agccatcagg
    11761 tgcctcaaaa caaaacgcct gctttttaag gcaggcgtct ttaacttcat tcataaattc
    11821 agggttgcgg gaatttctca ccacacgctg catataccgg agaatttttt cactcacact
    11881 ctcaccacaa aaaagaacaa tgtgactgtg ctgtgtcttc agcgacagaa ggatgacgga
    11941 gaacgcttcc gataaccaac acggctggcg ttctccgcaa aaaataaatt accagccgac
    12001 agacataccc gcagcaacac ctgaaccgtt ctgggtatcc catgaagcat tcaaacgtac
    12061 agcagaattt tcagtggtgc ggaagtgcat acctgctgcg atagcctgct catcacggta
    12121 gttacttgca gccataccaa aggagtacgt tttaccctca acaacaggaa tggcagtcat
    12181 cgccattgct cctgcaatac ctgcatctgc acgtttacgg tttttctcca cctcatcacg
    12241 taactgacgg aattgctgat tagtactacg ctccagggta ctgacccggt tatctgtgta
    12301 ggtattagca gacttcagtg tctgtgcatc accctgaata cgggcattct cttctgcaat
    12361 acgatagcgc tctaactgac cataggttgc ggcatccgca gcctgaacac catcacgaag
    12421 cccaccaatt acacgattcc ccacattaac ttcatcgggg cgagtggtct gagagttagc
    12481 ccctacagca acactgttcg tatggtttgc aatggcgttg taaccgacag cagtggcgtt
    12541 ttctccgcct gccgttgccc cgccaccaac ctgagtttta ccggtgccca gagaagacag
    12601 acgggcgtga atatctgtta catctgcagc agaaagagtg ctatcaatag tgcctcctgg
    12661 tattacagat tttattttaa tctcataaat atattcactg atttcaggga tgtgtcttga
    12721 gtacgctttt ccgtcagccc cgaccgtcgt gacaagtccc tcaccatctg cactaaacag
    12781 aatttgattt gttttggtgt ttacaaaata agcaacctca tttgcatagt cgtaataaga
    12841 tgctatttca ctacgcggtg ctaataattc tgtattcgtc aaaccagatt taccaacatc
    12901 gtaaagagaa tactttaaat atttccctgc cgtgttttgt atatttacac tttcaggttt
    12961 tctcgtgtct gcatttacca caactccatc cagataccct tttcctgaga agccgaggtc
    13021 tctccaaaaa tcaattggag ttttatattc atgtcctttc aactgatcaa cagtcactcc
    13081 ataatatgaa gtgccattaa ttccagaggc gtctgctgca tatacgccag cagaaaatac
    13141 cgacaggctt aatgctggca ttaaagccaa caatagcttg cttgcctttt tcatgatgtg
    13201 tacccttatg ttaaatcatg ttataaatat gttgacattc ggaacgcaaa tgtcttcagg
    13261 gatactactc acaataacaa ggcaaataaa atattaaaat caatgagtta agaaaaatta
    13321 ataattaaaa tcacaagaat gcaatacgca tcaaaaactt accctaaata acaccagtat
    13381 tactcagaaa aaacaaaaca ttttctgtgg ttattattta ccattgataa atattacctc
    13441 tctctgtcag cctgcattgt ataaaacata cactacaaac gggtgccata tcggctgaac
    13501 ctcagcaacc agttgacggg gactttcgtc cccgtcgcgg ttttcctact gcttacactg
    13561 taagaacgcc gcaaactccg ctccccacaa attcagccgg aactcacaca gcgaaccgta
    13621 caacatccag atagtgaaga taaccgtcat acaaattgtg acggtgataa gcgatttttg
    13681 cgacatagcg cttgactcct tttacagaga ggcgctaacc tt
//
LOCUS       DAZGAP010000148        13721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGAP010000148 DAZGAP010000000
VERSION     DAZGAP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.149"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            352..1461
                     /gene="livK"
                     /locus_tag="ACHLAM_003839"
     CDS             352..1461
                     /gene="livK"
                     /locus_tag="ACHLAM_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO7855297.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1509..2435
                     /gene="livH"
                     /locus_tag="ACHLAM_003840"
     CDS             1509..2435
                     /gene="livH"
                     /locus_tag="ACHLAM_003840"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO7855298.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2432..3709
                     /gene="livM"
                     /locus_tag="ACHLAM_003841"
     CDS             2432..3709
                     /gene="livM"
                     /locus_tag="ACHLAM_003841"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO7855299.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGSFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3706..4473
                     /gene="livG"
                     /locus_tag="ACHLAM_003842"
     CDS             3706..4473
                     /gene="livG"
                     /locus_tag="ACHLAM_003842"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO7855300.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4475..5188
                     /gene="livF"
                     /locus_tag="ACHLAM_003843"
     CDS             4475..5188
                     /gene="livF"
                     /locus_tag="ACHLAM_003843"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO7855301.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5276..5497
                     /locus_tag="ACHLAM_003844"
     CDS             5276..5497
                     /locus_tag="ACHLAM_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7855302.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            5494..5871
                     /locus_tag="ACHLAM_003845"
     CDS             5494..5871
                     /locus_tag="ACHLAM_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO7855303.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTSGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            6080..7396
                     /gene="ugpB"
                     /locus_tag="ACHLAM_003846"
     CDS             6080..7396
                     /gene="ugpB"
                     /locus_tag="ACHLAM_003846"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO7855304.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            7572..8459
                     /gene="ugpA"
                     /locus_tag="ACHLAM_003847"
     CDS             7572..8459
                     /gene="ugpA"
                     /locus_tag="ACHLAM_003847"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO7855305.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8456..9301
                     /gene="ugpE"
                     /locus_tag="ACHLAM_003848"
     CDS             8456..9301
                     /gene="ugpE"
                     /locus_tag="ACHLAM_003848"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO7855306.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9303..10373
                     /gene="ugpC"
                     /locus_tag="ACHLAM_003849"
     CDS             9303..10373
                     /gene="ugpC"
                     /locus_tag="ACHLAM_003849"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO7855307.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLKGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVLRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10370..11113
                     /gene="ugpQ"
                     /locus_tag="ACHLAM_003850"
     CDS             10370..11113
                     /gene="ugpQ"
                     /locus_tag="ACHLAM_003850"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7855308.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11100..11540)
                     /locus_tag="ACHLAM_003851"
     CDS             complement(11100..11540)
                     /locus_tag="ACHLAM_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO7855309.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11660..13405
                     /gene="ggt"
                     /locus_tag="ACHLAM_003852"
     CDS             11660..13405
                     /gene="ggt"
                     /locus_tag="ACHLAM_003852"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO7855310.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            complement(13443..>13721)
                     /locus_tag="ACHLAM_003853"
     CDS             complement(13443..>13721)
                     /locus_tag="ACHLAM_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO7855311.1"
                     /translation="TYHDAFAKANHYLDDADLPVVITLHGRFIQGWYFCFESQEFLES
                     GDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         3082 a         3438 c         3911 g         3290 t
ORIGIN      
        1 aattcttttg gcaggtgatt aattttttaa cagcaataat tacaaagtta aaacattaga
       61 gaatgaaaaa tacccagcat aatcccctga atggtagtga attattccgc cctttgtacc
      121 gttattttat gctgacaacg gcgctttttt ctgtttatct atcaataaat tcagaatatt
      181 atctgttctt aatcgactga aaaataggga ttttaatcgc tatcatcaca aaatactgtg
      241 ctaaaccctt aatcagacag gcaaaaacag tgcagtataa aaaaagaaca gtctgatttg
      301 ttaacacata agaacaaagc aacacaacat cacgaatggg gatttttgac tatgaaacgg
      361 aatgcgaaaa ctatcatcgc agggatgatt gcactggcaa tttcacacac cgctatggct
      421 gacgatatta aagtcgccgt tgtcggcgcg atgtccggcc cgattgccca gtggggcgat
      481 atggaattta acggagcgcg tcaggcgatt aaagacatta atgccaaagg gggaattaaa
      541 ggcgataagc tggttggcgt ggaatatgac gacgcctgcg acccgaaaca agccgttgcg
      601 gtcgccaaca aaatcgttaa cgacggcatt aaatacgtta ttgggcacct gtgttcctcc
      661 tctacccaac ctgcatcaga tatctacgaa gacgaaggta ttctgatgat ttcgccggga
      721 gcgaccaatc cggagctgac ccaacgcggt tatcaataca tcatgcgtac tgccggtctg
      781 gactcttccc aggggccaac ggcggcaaaa tacattcttg agacggtgaa gccccagcgc
      841 atcgccatca ttcacgacaa acaacagtat ggcgaagggc tggcgcgttc ggtgcaggac
      901 ggattgaaag cagccaatgc caacgtcgtc ttcttcgacg gcattaccgc tggtgagaaa
      961 gatttctccg cgctgatcgc ccgcctgaaa aaagaaaaca tcgacttcgt ttactacggc
     1021 ggttactacc cggaaatggg gcagatgctg cgccaggccc gttccgttgg cctgaaaact
     1081 cagtttatgg ggccggaagg ggtgggtaat gcgtcgctgt cgaacattgc tggtgatgcc
     1141 gccgaaggta tgttggtcac tatgccaaaa cgctatgacc aggacccggc aaacaaaggc
     1201 atcgttgatg cgctgaaagc agacaagaaa gatccgtccg gaccttatgt ctggatcacc
     1261 tacgcggcgg tgcaatctct ggcgactgcc cttgagcgta ccggcagcga tgagccgctg
     1321 gcgctggtga aagatttaaa agctaacggt gcaaacaccg tgattgggcc gctgaactgg
     1381 gatgaaaaag gcgatcttaa gggatttgat tttggtgtct tccagtggca cgccgacggt
     1441 tcatcgacgg cagccaagtg atcatcccac cgcccgtgaa atgcgggcgg ttttagaaag
     1501 gttaccttat gtctgagcag tttttgtatt tcttgcagca gatgtttaac ggcgtcacgc
     1561 tgggcagtac ctacgcgctg atagccatcg gctacaccat ggtttacggc attatcggca
     1621 tgatcaactt cgcccacggc gaggtttata tgattggtag ctatgtctcc tttatgatca
     1681 tcgccgcact gatgatgatg ggcattgata ccggctggct gctggtggcg gcgggattcg
     1741 tcggcgcaat cgtcattgcc agcgcctacg gctggagtat cgaacgggta gcctatcgtc
     1801 cggtgcgtaa ctctaagcgc ctgattgcgc ttatttccgc aatcggcatg tctatcttcc
     1861 tgcaaaacta cgtcagcctg accgaaggtt cgcgcgacgt ggcgctgccc agcctgttta
     1921 acggtcagtg ggtggtgggc catagcgaaa acttctctgc ctctattacc accatgcagg
     1981 cggtgatatg gattgttact ttcctcgcca tgctggcgct gacgattttc attcgctatt
     2041 cccgtatggg gcgcgcctgc cgtgcctgcg cggaagatct gaaaatggcg agcctgcttg
     2101 gcatcaacac cgaccgggtg attgcgctga cctttgtgat tggcgcggcg atggcggcag
     2161 tggcgggcgt gctgctcggt cagttctacg gcgtaattaa cccttacatc ggctttatgg
     2221 ccgggatgaa agcctttacc gctgcggtgc tcggtgggat tggcagcatt ccgggggcga
     2281 tgattggcgg gctgatcctg gggattgcgg aggcgctctc ttctgcctat ctgagtacgg
     2341 aatataaaga tgtggtctca ttcgccctgc tgatcctggt gctgctggtg atgccgaccg
     2401 gtattctggg tcgcccggag gtagagaaag tatgaaaccg atgcatattg caatggcgct
     2461 gctctctgcc gctatgttct ttgtgctggc gggcgtcttt atgggcgtgc aactggagct
     2521 ggatggcacc aaactggtgg tcgacacggc ttcagagatc cgttggcagt gggtgtttat
     2581 cggcacggcg gtggtctttt tcttccagct tgtgcggcct gctttccaga aagggctaaa
     2641 aagcgtttcc gggccgaagt ttattctgcc cgcgattgat ggctccacgg tgaagcagaa
     2701 actgttcctc gtggcgctgc tggtgcttgc agtggcgtgg ccgtttatgg tgtcgcgtgg
     2761 gacggtggat atcgccactc tgaccatgat ctacattatt ctcggtctcg ggctgaacgt
     2821 ggttgttggt ctttctggtc tgctggtgct gggatacggc agcttttacg ccatcggcgc
     2881 ttacactttt gcgctgctca atcactatta cggtctgggc ttctggacct gtctgccgat
     2941 tgccgggttg atggcggcgg cggcgggctt cctgctcggt tttccggtgc tgcgtctgcg
     3001 cggtgactat ctggcgatcg ttaccctcgg gttcggcgaa attgtgcgca tattgctgct
     3061 caataacacc gaaattaccg gcggaccgaa cggtatcagt cagattccga aaccgacgct
     3121 cttcggactc gagttcagcc gtaccgcccg cgaaggcggc tgggacacgt tcagtaattt
     3181 ctttggcctg aaatacgatc cctccgatcg cgtcatcttc ctctacctgg tggcgttgct
     3241 gctggtggtg ttaagcctgt ttgtcattaa ccgcctgctg cggatgccgc tgggccgtgc
     3301 gtgggaagcg ttgcgtgaag acgagatcgc ctgccgttcg ctgggcttaa gcccgcgtcg
     3361 tatcaagctg actgccttta ccatcagcgc cgcgtttgct ggttttgccg gaacgctgtt
     3421 tgctgcgcgt cagggctttg tcagcccgga atccttcact tttgccgaat cggcgtttgt
     3481 gctggcgata gtggtgctcg gtggtatggg ttcgcaattt gcggtgattc tggcggcaat
     3541 tttgctggtg gtgtcgcgag agttgatgcg tgatttcaac gaatacagca tgttaatgct
     3601 cggtggtttg atggtgctga tgatgatctg gcgtccgcag ggcttgctgc ccatgacgcg
     3661 cccgcaactc aagctgaaaa acggcgcagc gaaaggagag caggcatgag tcagccatta
     3721 ttatctgtta acggcctgat gatgcgcttc ggcggcctgc tggcggtgaa taacgtcaat
     3781 cttgaactgt acccgcagga gatcgtctcg ttaatcggcc ctaacggtgc cggaaaaacc
     3841 acggttttta actgcctgac cggtttctac aaacccaccg gtggcactat tttactgcgc
     3901 gatcagcatc tggaaggttt gcccggccag caaatcgccc gcatgggcgt ggtgcgcacc
     3961 ttccagcatg tgcgtctgtt tcgtgaaatg acggtgattg aaaacttgct ggtggcgcag
     4021 catcagcaac tgaaaaccgg gctgttctcc ggcctgttga aaacgccttc tttccgccgc
     4081 gcccagagcg aagcgctcga ccgtgccgca acctggcttg agcgcattgg tttgctcgaa
     4141 cacgctaacc gtcaggcgag taacctggcc tacggtgacc agcgccgtct ggagatcgcc
     4201 cgctgcatgg taacgcagcc ggagatttta atgctcgacg aaccggcagc aggtcttaac
     4261 ccgaaagaga cgaaagagct ggatgagctg attgccgaac tgcgtaacca tcacaacacc
     4321 actatcttgt tgattgaaca cgatatgaag ctggtgatgg ggatttcaga cagaatttac
     4381 gtggtcaatc aggggacgcc gctggcaaac ggtacgccgg agcagatccg taataacccg
     4441 gacgtgatcc gtgcctattt aggtgaggca taagatggaa aaagtcatgt tgtcctttga
     4501 caaagttagc gcccactacg gcaaaatcca ggcgctgcat gaggtgagcc tgcatatcaa
     4561 tcagggcgaa attgtcacgc tgattggcgc gaacggggcg gggaaaacca ccttgctcgg
     4621 cacgttatgc ggcgacccac gcgccaccag cgggcgaatt gtgtttgatg ataaagacat
     4681 taccgactgg cagacggcga aaatcatgcg cgaagcggtg gcgattgtcc cggaaggacg
     4741 tcgcgtcttc tcacgcatga cggtggaaga gaatctggcg atgggcggct tttttgctga
     4801 acgcgaccag ttccaggagc gcataaagtg ggtgtatgaa ctgtttccac gtctgcatga
     4861 acgccgtgtt cagcgggcag gcaccatgtc cggcggtgaa cagcagatgc tggcgattgg
     4921 tcgcgctttg atgagtaacc cacgtttgtt attgcttgat gaaccctcgc tcggactggc
     4981 accgattatc atccagcaaa ttttcgacac catcgagcaa ctgcgcgagc aggggatgac
     5041 catcttcctc gtcgagcaga acgccaacca ggcgctgaag ctcgctgatc gcggctacgt
     5101 gctggaaaac ggtcatgtgg tgctctccga tactggcgat gcgctgctgg cgaacgaagc
     5161 ggtgagaagt gcgtatttag gcgggtaata atattttgag taaaaaggag tcgaatgact
     5221 ccttttttgt acttgttagt tgtacaattt aattgtactt aatatggagg atatcatgca
     5281 taccatgaac tacagcgaag cgcggcagaa ccttgcatta gttctggaaa cagcggcgac
     5341 aggtactccg gtaacgatca cgaggcgtgg gcataaggca gctgtgatta tcagcgcaga
     5401 agagtttgaa cgttaccaga aggctaaact ggacgcagaa tttgacgcca tcatggctat
     5461 tcatggcagc gaaattaagg agttagcgga taaatgacca ttcagtttat ctcagcggag
     5521 gagataattt atttccacga taaattgctt agcgttacat caggtgtaac aggaatgtcc
     5581 gatccagggc gagcagaagc gcttttgtac cgggtactga ataagtatga gtatgaaggc
     5641 gtttctgatc tttggctgct ggcggcgatg catctattgg caatctcgcg cggtcatatc
     5701 tttaatgatg gtaataagcg caccgcactg tttatcacgc tattattcct taaacgtaac
     5761 ggcatcatac ttgctgcaaa cccagagttt gtagaattga cggttgatgt ggcggcggga
     5821 aaagtatcgt tagaagatat cgttacccgc ttgcgtcaac gcggtacctg acaaggttct
     5881 tagccattct gctttttatc cgtgccccat ctcccatttt ccctcaccca cgccgtcacc
     5941 gccttgtcat ctttctgaca ccttactatc ttacaaatgt aacaaaaaag ttatttttct
     6001 gtaattcgag catgtcatgt taccccgcga gcataaaaag cgtgaattcg cgcatccggt
     6061 acaacaagag agataaacga tgaaaccgtt acattataca gcttcagcac tggcgctcgg
     6121 actggcgtta atggggaatg cacaggcagt gacgaccatt ccgttctggc attctatgga
     6181 aggggaactg ggtaaagagg tggattctct ggcccaacgt tttaacgccg aaaacccgga
     6241 ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag aatttaagcg cggggattgc
     6301 cgcatttcgt accggcaacg cgccggctat tttgcaggtt tatgaagttg gcaccgccac
     6361 catgatggcg tcgaaagcca ttaaaccggt atatgacgtg tttaaagagg cggggattca
     6421 gttcgatgag tcgcagtttg tgccgacggt ttcaggctac tactccgaca gcaaaaccgg
     6481 ccacttactc tcccagccgt tcaatagctc gactcctgtt ctctattaca acaaagacgc
     6541 cttcaagaaa gccggtttag acccggaaca gccgccgaaa acctggcagg atctggcgga
     6601 ctatgccgcg aaactgaaag cctccggtat gaagtgcggc tacgccagcg gctggcaggg
     6661 ctggatccaa ctggaaaact ttagcgcctg gaacggtctg ccgtttgcca gcaaaaacaa
     6721 cggctttgac ggcacagacg cggtgctgga gttcaacaag ccggagcagg tgaaacacat
     6781 cgctatgctc gaagagatga acaagaaggg cgatttcagc tacgtcgggc gtaaggatga
     6841 atccactgag aagttctata acggtgattg cgcgatgacc accgcctctt ccggttcgct
     6901 tgccaacatt cgtgagtacg ccaaatttaa ctacggcgta ggcatgatgc cttacgacgc
     6961 cgatgcgaaa gatgcaccgc aaaacgccat tatcggcggg gccagcctgt gggtgatgca
     7021 gggcaaagat aaagaaacgt ataccggcgt ggcgaagttc ctcgacttcc tcgcgaagcc
     7081 agaaaacgct gccgagtggc atcagaaaac cggttatctg ccaatcacca aagcggcgta
     7141 tgacctgacc cgtgagcagg gcttttatga gaaaaaccca ggggcggata ccgcgacgcg
     7201 tcagatgctg aataagccgc cgttaccgtt caccaaaggg ctgcgtctgg gcaacatgcc
     7261 gcagatccgc gtgattgtgg atgaagagct ggagagcgtg tggaccggta agaagacacc
     7321 gcagcaggcg ctggataccg ccgttgagcg tgggaaccag ttactgcgcc gctttgagca
     7381 gtctacgaag tcttaattct cttaccctca ccctaaccct ctcccaaagg gagaggggac
     7441 cgatcggcgt ggtatcctgg tgccgtgtcg gtttgcaact tcgctcggtg ggaaagtatg
     7501 caccgccttt ctctctcgcc cctctgggga gagggccggg gtgaggggaa cacaacacga
     7561 agagtttaac tatgtcatca tcccgtccgg tgttccgctc gcgctggctg ccctatttgc
     7621 tggtcgcgcc gcagctcatc atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt
     7681 tgtggtactc gctgcaaagc gtcgatccgt ttggtttctc cagccagttt gtcggcctgg
     7741 ataacttcgt cacgctgttt catgacagct actatctcga ctccttctgg acgacgataa
     7801 aattcagcac ctttgtcacc gtcagcggtt tgctggtgtc gctgttcttt gcggcgctgg
     7861 tggagtacat cgtgcgcggc agccgtttct atcaaacctt aatgctgctg ccttacgctg
     7921 tggctcccgc cgttgccgcc gtattgtgga tcttcctgtt taaccccggt cgcgggctga
     7981 tcacccattt tctcgctgag ttcggctacg actggaacca cgcgcaaaac agcggtcagg
     8041 cgatgtttct ggtggtgttt gcctcggtgt ggaagcaaat cagctacaac tttctgttct
     8101 tctatgccgc gctgcaatcc attccccgtt cgttgatcga agccgcagcc atcgacggtg
     8161 caggcccgat tcgccgcttc tttaagattg cgctgccgct tatcgctccg gtgagtttct
     8221 tcctgctggt ggtgaacctg gtgtacgcct tcttcgatac cttcccggtg atcgacgccg
     8281 ccacgtccgg cgggccggtt caggccacca cgacgctgat ttataagatt taccgcgaag
     8341 gctttaccgg actggatctg gcttcgtctg ccgcgcagtc ggtggtgttg atgttcctcg
     8401 tcatcgtgct gacggtggtg cagttccgct atgttgaaag caaggtgcgt taccaatgat
     8461 tgagaaccgt ccgtggctga caatattcag ccataccatg ctgatcctcg ggatcgcggt
     8521 gatcctcttc ccgctgtacg tggcgtttgt cgcggcgaca ctggataaac aagccgtcta
     8581 cgccgcgccg atgacgctaa tccccggcac gcatctgctg gaaaacatcc acaacatctg
     8641 ggtgaacggg gtaggcacga atagcgcgcc gttctggcgg atgttgctta acagctttgt
     8701 gatggcgttc agcattacgc tcggcaaaat taccgtctcg atgctctcgg cctttgccat
     8761 tgtctggttt cgttttccac tacgtaacct cttcttctgg atgattttta tcaccctgat
     8821 gctgccggtt gaagtgcgta tcttcccgac ggtggaagtc atcgccaacc tgaaaatgct
     8881 cgacagctac gcaggtttaa cgctgccgct gatggcctcg gcgaccgcca cgtttctgtt
     8941 tcgccagttc tttatgaccc taccggatga actggtggaa gccgcgcgga tcgacggcgc
     9001 gtcaccaatg cgtttctttt gcgacatcgt ttttccgctg tccaaaacca atctggcggc
     9061 gctgtttgtg atcaccttta tctacggctg gaaccagtat ttgtggccgt tgttgattat
     9121 taccgacgtt gatctcggca ccaccgtggc agggattaaa gggatgatcg ccacaggcga
     9181 aggcacaacg gaatggaact cggtgatggc ggcgatgttg ttaaccctga tcccaccagt
     9241 agtgattgtt ttagtgatgc agcgtgcctt cgtgcgcggc ctggtcgata gtgagaaata
     9301 agatggcagg actgaaatta caggcagtaa ccaaaagctg ggatggcaaa acccaggtca
     9361 ttaaaccgct gacccttgat gtggcggatg gcgaatttat cgtgatggtc gggccgtctg
     9421 gctgcgggaa atcgacgtta ttgcgaatgg ttgccggact ggagcgggta acgacgggcg
     9481 atatctggat cgaccgcaag cgtgtcaccg aaatggagcc gaaagatcgc gggattgcga
     9541 tggtgttcca gaactacgcg ctttatccgc atatgagcgt cgaagaaaac atggcgtggg
     9601 ggctgaaaat tcgcggcatg ggcaagcagc aaattgccga gcgcgttaaa gaagcggcgc
     9661 gcattctgga gctggacggc ctgcttaagc gccgcccgcg tgagctatct ggcggtcagc
     9721 gtcagcgtgt ggcgatgggg cgtgcgattg tgcgtgagcc ggcagtgttc ctgtttgatg
     9781 agccgctctc taacctcgat gccaagttgc gtgtgcagat gcgtcttgaa ctgcaacagc
     9841 tgcaccgtcg cctgaaaacg acttcactct acgttaccca cgatcaggtt gaggcgatga
     9901 cgctcgccca gcgagtaatg gtgatgaacg gcggcgttgc cgaacagatt ggcacgccag
     9961 ttgaagtgta cgaaaagccc gccagcctgt ttgtggcgag ttttatcggc agtccggcga
    10021 tgaatctgct gaaagggcgt gtgaataacg aaggtacgca tttcgaactg gacggcggta
    10081 tcgcgctgcc gctaaacggt ggctaccgtc agtatgctgg acgtaaaatg actctcggca
    10141 ttcgcccgga acatattacg ctacgttcgc aggctgaagg tggcgtgccg ctggtgatgg
    10201 acacgctgga gatcctcggc gcagataacc tggcgcacgg acgctggggc gaacagaagc
    10261 tggtgttgcg actggcgcat caggagcgcc cgacggcagg cagcacgctg tggctgcatc
    10321 tgccggaaaa tcagctacat ctttttgatg gtgaaacagg acaacgagta tgagtaactg
    10381 gccttatccc cgcatcgtcg cccatcgtgg cggcggtaaa ctggccccgg aaaacaccct
    10441 ggcggcaatc gatgtggggg caagatacgg tcataagatg atcgaatttg acgcgaagtt
    10501 atcgaaagat ggcgagatct tcttgcttca tgacgacaat ctcgaacgca ccagcaacgg
    10561 ctggggcgtc gcgggtgaac tgaactggca ggatttactg cgcgtggatg cgggcagttg
    10621 gtacagcaaa gcgtttaaag gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg
    10681 tcgcgaacac gggatgatgg cgaatatcga aatcaaaccc accactggca ccgggccatt
    10741 aacgggcaaa acggtggcgc tggcggcacg cgaactgtgg accggtatga cgccgccgct
    10801 gctgtcatcg tttgagattg atgctttaga agccgcgcaa caggcagcac cggaactgcc
    10861 gcgtggttta ttgcttgatg aatggcgcga cgactggcga gaattgaccg cgcggctggg
    10921 ctgcgtctct attcatctca atcataagtt gctcgataaa gcgcgagtga tgcagttgaa
    10981 agacgccgga ttacggattc tggtttatac cgtcaataaa ccccagcgcg cggcagagtt
    11041 gctgcgctgg ggcgtggatt gcatctgtac cgatgcgatt gacgtgattg gtccgaactt
    11101 tacggcccaa tagttttcaa cggaatatca ggctgcggcg tgctgggctg attcagcatg
    11161 tcgccgtttc tcctttccgg cagcatatgc tgctgattca acgaactatc cggattacgg
    11221 ttgctgttta acatcccgcc attggtgttg ggcagcattt gctgtcgggc gggatttcgc
    11281 tcccccggct gtgactgcaa cacccgctga gaattattgt ttatctggtt ttctaaatgc
    11341 tgctgttgca gctgcgtttg cgttttcagt tgctgattca gcatcccttt ttgctggatt
    11401 tgctgcgttt gcatttgggt ttgcatccgc tgctgactgg ggatctgata ccccggctgg
    11461 ttagggttat tcagagtatt aatgggctgt gcaaagccga caaacggcag gagtgccgta
    11521 agaatcagaa gtcgtttcat cgcgtatcct cctctgaaga tattctttaa gtttactcct
    11581 ttcctgcaat catcaggatt aattcagagt tatataccag gcttagctgg ggttgcccct
    11641 taatctctgg agaataacga tgataaaacc gacgttttta cgccgggtgg ccattgctgc
    11701 tctgttcaca ggaagttgtt ttagtacggt agccgcgcca cctgcgccgt ctcctgtttc
    11761 ttacggcgtg gaggaagatg tctttcatcc ggtacgcgcg aaacagggga tggtggcgtc
    11821 tgtagatgct actgccactc aggtgggggt ggatatcctc aaggagggcg ggaatgccgt
    11881 tgatgccgcc gtggctgtgg gctacgcgct ggcggtaacg catccgcagg cagggaatct
    11941 gggcggtggt ggttttatgc tgatccgctc gaaaaatggc aataccaccg ctatcgattt
    12001 ccgcgaaatg gctcccgcca aagcgacccg cgatatgttc cttgatgatc agggtaaccc
    12061 ggacagcaaa aaatcactca cttcgcatct ggcttctggt acgcccggta cggtagcagg
    12121 tttctcgctg gcgttggata aatacggcac catgccgttg aacaaagtgg tgcaaccggc
    12181 gtttaaactg gcacgcgatg gttttatcgt taacgacgcg ttggctgacg atctcaaaac
    12241 ctacggtagc gaagtgttgc cgaatcacga aaacagcaag gctatcttct ggaaagaggg
    12301 cgcgccgctg aaaaagggcg acaagctggt gcaggcgaac ctggcaaaga gcctggagat
    12361 gattgccgaa aacggcccgg acgaattcta caaaggcacg atagcggaac agattgccca
    12421 ggagatgcag aaaaacggtg gcttgatcac taaagaagat ttagcggcgt ataaagcggt
    12481 cgaacgtaca ccgattagcg gcgaatatcg cgggtatcag gtttactcga tgccaccgcc
    12541 atcttccggt gggattcata tcgtgcagat cctcaatatc ctggaaaact tcgatatgca
    12601 gaaatacggc tttggcagcg ccgatgcgat gcaaatcatg gcagaagcgg agaaatacgc
    12661 ctacgccgac cgctcggaat atcttggcga tccggatttt gtcaaagtcc cgtggcaggc
    12721 gctgaccaat aaagcctatg ccaaatctat tgccgatcaa atcgatatca ataaagcgaa
    12781 gccatccagc gaaattcgcc ctggcaagct tgcgccttat gagagtaatc aaactaccca
    12841 ttactcagtg gtggataaag atggtaacgc ggtggcggtg acctatacgc tgaacaccac
    12901 cttcggtacg ggcattgtcg cgggcgagag cggtattctg cttaataacc aaatggatga
    12961 tttctccgcc aaaccgggcg tgccgaacgt ttacgggctg gtgggtggtg acgctaacgc
    13021 ggtcgggccg aataaacgtc cgctgtcgtc gatgtcacca accattgtgg tgaaagacgg
    13081 taaaacctgg ctggtcaccg gaagcccagg cggtagccgg atcatcacta ccgtgctgca
    13141 aatggtggtg aatagcatcg attatggtat gaatgtcgcc gaagcgacca atgcaccgcg
    13201 tttccaccat cagtggttgc cggatgagct gcgtgtcgag aaaggattta gcccggatac
    13261 gctcaagctg ctggaagcaa aaggtcagaa agtggcgctg aaagaggcga tgggcagtac
    13321 acaaagcatt atggttgggc ctgacggtga gttgtacggc gcatccgacc cgcgctcagt
    13381 agatgattta acggcggggt attaaggtta gcggccctct tcgtgggaag agggccgtat
    13441 tgtcagggca agccgaaggt agcctttttt atttcataat cctgtagata ttcttccagc
    13501 ggctttgccg ttccaaggga atgaatttca ccactgtctt tatcaataat aaaaggtgcg
    13561 ttaccggcta agcgcgcggc ctcatcacca gattcaagaa attcttgtga ttcgaaacag
    13621 aaataccagc cctggataaa acgtccatgc agagtgatga cgacagggag atctgcatca
    13681 tcaaggtaat ggttcgcttt cgcgaatgcg tcgtgataag t
//
LOCUS       DAZGAP010000149        13492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGAP010000149 DAZGAP010000000
VERSION     DAZGAP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.150"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..829)
                     /gene="uxuR"
                     /locus_tag="ACHLAM_003854"
     CDS             complement(56..829)
                     /gene="uxuR"
                     /locus_tag="ACHLAM_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO7855312.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1044..2504)
                     /locus_tag="ACHLAM_003855"
     CDS             complement(1044..2504)
                     /locus_tag="ACHLAM_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO7855313.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2585..3769)
                     /gene="uxuA"
                     /locus_tag="ACHLAM_003856"
     CDS             complement(2585..3769)
                     /gene="uxuA"
                     /locus_tag="ACHLAM_003856"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO7855314.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIVGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4109..5452
                     /gene="gntP"
                     /locus_tag="ACHLAM_003857"
     CDS             4109..5452
                     /gene="gntP"
                     /locus_tag="ACHLAM_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO7855315.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5625..6527)
                     /gene="fimH"
                     /locus_tag="ACHLAM_003858"
     CDS             complement(5625..6527)
                     /gene="fimH"
                     /locus_tag="ACHLAM_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO7855316.1"
                     /translation="MKRVIYLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6547..7050)
                     /gene="fimG"
                     /locus_tag="ACHLAM_003859"
     CDS             complement(6547..7050)
                     /gene="fimG"
                     /locus_tag="ACHLAM_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO7855317.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTAGFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7063..7593)
                     /gene="fimF"
                     /locus_tag="ACHLAM_003860"
     CDS             complement(7063..7593)
                     /gene="fimF"
                     /locus_tag="ACHLAM_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO7855318.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7603..10239)
                     /gene="fimD"
                     /locus_tag="ACHLAM_003861"
     CDS             complement(7603..10239)
                     /gene="fimD"
                     /locus_tag="ACHLAM_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO7855319.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10306..11031)
                     /gene="fimC"
                     /locus_tag="ACHLAM_003862"
     CDS             complement(10306..11031)
                     /gene="fimC"
                     /locus_tag="ACHLAM_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO7855320.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11068..11607)
                     /locus_tag="ACHLAM_003863"
     CDS             complement(11068..11607)
                     /locus_tag="ACHLAM_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7855321.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGKISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11672..12226)
                     /gene="fimA"
                     /locus_tag="ACHLAM_003864"
     CDS             complement(11672..12226)
                     /gene="fimA"
                     /locus_tag="ACHLAM_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7855322.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTA
                     IDGTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
     gene            complement(12707..13303)
                     /gene="fimE"
                     /locus_tag="ACHLAM_003865"
     CDS             complement(12707..13303)
                     /gene="fimE"
                     /locus_tag="ACHLAM_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO7855323.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         3266 a         3491 c         3236 g         3499 t
ORIGIN      
        1 attcagggaa catacatagt gcaaagcggt tcaactttgc accagaaatg agggatcagg
       61 cgtcgacttt atccagcggc catgaatcaa acagatagcc gtcaaaataa atatcgtcaa
      121 cgttcgagaa ttccagcaga cgttgtttaa cgttttccag atgctgccac attgccagct
      181 tcgccgcacg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
      241 actctttgcg atacaggctg tcatcgagat ggctgtgcaa ttggatccac attggattgt
      301 tttcccgcca ctgccaggac tgacggaata actccaccag catgctgtta tgcgttgctt
      361 cggcaatggc gagatggaac tgcatatcac cgctttcgct gctacccggc gcgctggaag
      421 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
      481 cctgcaaagc ggcaaattcc gcgatgttac tttccagtaa ctgacgcgcc tgtaacagct
      541 caaaaggacc ggcatcatta cagacgttgg catccggact gtctgtgttg tggctgcctg
      601 agctatcaag aacataaata ccggcacccc ggcgcacttc caccagccct ttgatctcca
      661 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
      721 gctccggtgg cagccgttcg ccaggattgt acggcgtctt tatgatcaga tcgcggatca
      781 tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc
      841 cgttaggtgc ggttaatcaa attttttccg gatgcggcgt gaacgcctta tccgacctac
      901 aacgggcaat tttacggcac ccgttaagct caaactatac cattagcact ggatgttggc
      961 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tattagtttg attgaccagt
     1021 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgctca
     1081 cacagctgct gatatgccgc cgtcaccgcg ccaacaaagt cggcgttctg cggcagatca
     1141 tcggcaaaga taccgctcag gcccagcagt gctttcacgc ggtctgcacc ctgatactgc
     1201 gcgttgatct tctggaactc cgccagcatc gggtcaacca cgtcaatggc attaccctgc
     1261 tcatccacgc cctgggtgta acgcatccag cccgccacgc ccagcgccag gtgacgccag
     1321 ctaccgccgt tttgcaggtg cagacgcacc gggtccagca gacgctgcgg taacttctgg
     1381 ctgccgtcca ttgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     1441 atcagcagcg tcgcataagc gttcaggtct gtaccttccg gcatcgacag cgttggcgct
     1501 tgttcctgca tcatcagggc aaaggccgct ttgcgataag ccgggttagt catggtgtcg
     1561 gcaatagttt catagccgcc gaggtaaccg aggtacgcca ggaaagagtg gctaccgttc
     1621 agcatacgca gcttcatcat ttcgaacggt acaacatccg caacaaactg cgcgccgact
     1681 ttatcccagt ccggacgacc attaacgaag ttatcttcaa ttacccactg acggaacggt
     1741 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt
     1801 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttctct
     1861 tcaatccatg ccgccagttg cgggtcacgc gcctgagcca gcccaagaac ggcaaccttc
     1921 gccacatgac cgttttcacg cacgttatcg caggacatca ccgtaaatgc tttcagccct
     1981 ttttcacgac gcagacgcag ggcttctacg atgtaaccaa tcgcagactt cggcgcagtc
     2041 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc gcttgccgca
     2101 tctgcgcagt agcctttttc cgtgaccgtc agagagacaa tcgccgtctg cggacgcgcc
     2161 atcgcgttga gaataccttc gcaaccgtcg atttccgggt gcagggcttc tttcatcgaa
     2221 ccgataattt tcagctcggt gctttctgcg cctttttccg ccacggtgta cagcagttgc
     2281 tgttttttca ggttttcgat cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag
     2341 atcccccagt cgctatccgt gctttccagc aggtgatgag tgtacagcgc ctggtgcgcg
     2401 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     2461 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     2521 cgtcttcaga gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc catgccggtg
     2581 gatattaacg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     2641 ggcctttcag acgaccaatt gcggagtaac caggattggt tttcttcttc aggtcgtcca
     2701 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     2761 gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg tcaccgttca
     2821 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     2881 tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg tcagcacgca
     2941 cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     3001 tccactgcat atcttcaatg gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg
     3061 gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc agcaactgga ataatcgctt
     3121 tcaggaagac agcgaagttt tcgcgcagct tcgctttgtc gatatctttg tacagctcca
     3181 ggtgcttacg gaactggtcg agggtatacc cttcttccgc acctggcaga ccgacaatga
     3241 tgttacgggt cagacgcgct ttgtcttcgt cgctcatagt ggcgaagcga acagctgcct
     3301 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgcttcagg atatgcattt
     3361 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     3421 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     3481 tgccgcactg cgccaggttg cgcagggtct gctgatagtt agcaatccac tgctcatagt
     3541 tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct ttcaacgaca gaccacacca
     3601 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     3661 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     3721 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     3781 tttcacatag tgggttccac agcaaagaga cgtggagccc cggagttgtt taggtatccg
     3841 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     3901 cttttggtaa gacatgtcaa cccgaaatct gaaattggtt aaccacatca caagaatttc
     3961 actctcctgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     4021 atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc caaaataaca
     4081 acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
     4141 cggcattggt ctgatgctgg tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     4201 gttggtggcg gcgctgtccg tcgggatgct ggcgggcatg gatttgatgt cgctgctgca
     4261 caccatgaaa gcgggcttcg gcaacacgct gggggaactg gctatcatcg tggtgttcgg
     4321 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cacatacgct
     4381 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg gcctgatttt
     4441 cggtctggcg atgttttatg aagtggcctt tatcatgtta gcgccgctgg ttattgttat
     4501 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
     4561 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcactggtga atgcttatgg
     4621 cgcggatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtcatctg
     4681 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     4741 gaaagcagat caaccggtag atatgaataa tctgccctct ttcggcgttt cgattctggt
     4801 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
     4861 agatacccct gcctgggaag tggttaactt tatcggttcc tcgccgattg caatgtttat
     4921 tgcgatggtg gttgcattcg tactctttgg caccgcgcgt ggtcatgaca tgcagtgggt
     4981 gatgaacgct tttgaaagcg cggtgaaaag cattgcaatg gtgattctga tcatcggtgc
     5041 gggtggcgtg ctgaagcaga ccatcatcga caccggcatt ggcgacacca tcggcatgtt
     5101 gatgtcccac ggcaatatct cgccctacat catggcatgg ctgatcaccg tactgattcg
     5161 tctggcgacg ggtcagggtg tcgtttcggc gatgaccgct gccgggatta tcagtgctgc
     5221 aatccttgat ccagcaaccg gtcagctggt tggcgtgaat ccggcgctgc tggtgctggc
     5281 gacggctgcg ggttccaaca ccctcacgca cattaacgat gcatctttct ggttgttcaa
     5341 aggttacttt gacctgtcag taaaagacac gttgaaaacc tggggtctgc tggagctggt
     5401 caactccgtg gttgggctga ttattgtgtt gattattagc atggtagcgt aaaagaacac
     5461 cggagcctgc caatggcagg ctctgttcgg attgtcggta aagtgcgctt tgtttatgcc
     5521 agatgcgacg ctgacgcgtc ttatctggca tacaaagggc taacgtgcag gttttagctt
     5581 caggtaatat tgcgtacctg cattagcaat gccctgtgat ttctttattg ataaacaaaa
     5641 gtaacgccaa taatcgattg cacattccct gcagtcacct gccctccggt acgtgcgtaa
     5701 tttgccgtta atcccagact taccgccgaa gtccccactg ctcctaacga taccgtgtta
     5761 ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg tgctggtgaa
     5821 aacgaggcgg tattggtgaa aatcgagttg cccgcatctg cggttgtgcc ggagaggtaa
     5881 taccccaggt tttggctttt cgcacaataa acggtaagag gaatcggcac cgaaccggga
     5941 tagtccggca gagtaacggt gacatcacga gcagaaacat cacagccgcc agtaggcacc
     6001 accacatcat tattggcgta aatattccac acaaactgga aatcatcgct gttatagttg
     6061 ttggtctgtc gcaaaattag cacggcaatt aatgagccag ctttaatcgc caccccgccc
     6121 gcactgctca caggcgtcaa ataaagcgcc accggccacg gcttatccgt tctcgaatta
     6181 taaacaacgc gcggcgtttc gctagtggtc ggaaatggat agctactgcc actatatttt
     6241 acggtcccgg aaaaattaga taacacgccg ccataagccg agcctcgttg cagtgtgaca
     6301 tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga tttgcgtcga aagatccacg
     6361 accaggtttt gccccacatt cacggcaggc gcaaggttta cataaacatt agcgctgcca
     6421 ccgccaatag ggatagcggt gccattggcg gttttacagg cgaatgacca ggcatttacc
     6481 gaccagccca tcagcagtac agcaaacagg taaataactc gtttcattac aatcatctct
     6541 tcggcttcag ctgtaggtat aggtgatgct aattactgcc tgaatggttc cctgagtggc
     6601 tccgccattt accgtcaatg ctctgacctg taacgggaag tgcgctgatt gtgaggaatc
     6661 atccacctga actgttttgg ttgcgccact attcaatgtg ttgccactgt catcctgtag
     6721 ctctaactgg atgttttgcg cggtcccctg gtttttataa tatccggtac tgtcggctgc
     6781 cccgctgaag ccggcggtga ccctcgacgt tcccaccgga caattagtca actcaagcgc
     6841 aacatcatgc caggccgatg ccgccccggc agacatcaga ctgaaagaat aaagatcgcc
     6901 gagatctacc gtggcattgg tggtggaaac cgtgcacggt ttggcgacga ccttaccgtt
     6961 caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt gcgagcgcca acattgccgc
     7021 caataaatac ccacgtttgc gccatttcat gagcatctcc agttactgat attcaagagt
     7081 gaaggtagcc gtggcattga tatgccccgc agtgacaggc acctgtgtcg ccattagccg
     7141 ggcgtaaaaa ttcagcgtat ttggtttacc cggcgtcagg gtcgtccacg aaatcgcgga
     7201 cgatggggca ttaaggggta tctgattttg ttgctcattc agaagctgta ttcccagtcc
     7261 cgcagccgct gacaccgtat tttcaagtgc aagcaggttg gcattgtggc tatctgcaac
     7321 accggtaaac ccaactttta cggcagaaac ggcattacca cagggtgaca gcaaaatacg
     7381 aaatggaacg acaggagtcg tcgcgccaat gttgttaaat tgcttcaccg cgttttccat
     7441 cagatcaaca gtaaaattgg ttgattcagc ggccacacta cagccattat ctctgacata
     7501 gccgcggata gtaatcgtgc tatccgcagc caaagcgtga cttaccgcca gccacaaaaa
     7561 agcgcaaaga agataaaaag gtttgtttct catcacgcct ccttaacgac attcagctga
     7621 tagctgggtt aataactgct gctgactctc tggtggcagt tgataattgg cgacacagtg
     7681 agcattttcc tcttctcccc atttcacctg aacttttccc gctaaaggca ttccgctgag
     7741 gtaaacctga ccattatccg caacaatgcc gctactctgg ctactctctg atgtcaccat
     7801 cgccccaaac ggcagcggct tattattgtg ggttagcgtc atgagcagtt ttatcccaac
     7861 gcgcgcttta aactctgctc gcacgatcgc cccacgggtg ggaacaacgt tagcgaccgc
     7921 gttatctaaa tcgacgttat cagccagggt attggtatcc agcgctactc tgttttcccg
     7981 atattcagtg gcataaggca gcacggcata accgcgccag tcggtacgca cccccgtctg
     8041 gttttcgact tttgcatctt ttgcgccagg cgctttaaca agcaccaccg tatcgtttaa
     8101 cggctgcccc agcgttacgc cattggcatg agccagtacc ccaccgctga ctccgtaata
     8161 gagctgctta atatcatcgc tatggctgta accgatgttg gcattgccgt aaccaccgcg
     8221 ataattcagc gtggcgtagc ctgtacttcc gctattacca tcacctcccc cggcgtaacc
     8281 ggtttgcacg ctatagctga ggttgttgtc ttccagcaac gtaccgtata caccggccag
     8341 attggtcatc cgaccgttga gatcgtgtga catgctgtaa ctggcactgg catgtcgcca
     8401 ctgagattta ctgtcagaac gcagccagtg gctgaaagga atattgacgt taagcgctaa
     8461 catctgatcc cgtccttttt gccaggcgtt tttcgtcagg ctatagctga gcgtccagtt
     8521 gatatcttcg aatgcagtat ttaaaccagc ctggaattgc tcatcgacat tactcgttcc
     8581 ccaataagtt tgatggctac cactcaaata cagtgttgat gagcgcccga gttgctgagt
     8641 aacggtgagc tgtaatttcc cgcgtttgtt ataagcgaga ttgtaatagt cggtgaattt
     8701 cggcttaacc tgaataactc catcctgtgt ttcgatgttg tagccattca ttcgactgta
     8761 tgttgtatca gcgaaattaa aatacccact ggtcgaataa cggtaaccca ctaactgaat
     8821 attcgtgcct gactcattga gcgatttgtt ataaagaaaa cgcaccgatt gtccgtcatg
     8881 ctgactgtca tcgggaagtg tggaattagc ctgcgtcata tccacagaca gagcgcccag
     8941 tgcccccata tttttcccga taccgaaatt aaacgcacga taacgatccg ccagttgcgt
     9001 tccaccatat attgtccagc cagccggaag gccgtggagt aatgtactct ggaaaaagcg
     9061 gggtttttcc tgttgcgcat ttccactacg gtattctcct gccgtaatgg aataacgtgt
     9121 atgcccttca cgctgcaaaa gcgggactga cgaatagggt acggtaaaaa tctgcgtact
     9181 gccgtcagcc tctttaatcg ttacctgcaa gtcaccacta ttacctgcgg catagatatc
     9241 gttgatggta aaaggccccg gtggcaccgt actattataa atgtcatacc cattttgttt
     9301 aatagtgacc tgtgcagtac cacgagcaat accgtggatc accggggcaa atcctctttg
     9361 gctatcgggt aacatattgt catctgaggc caattgtgcg ccgcgaaagt taataccatc
     9421 gaaaatatcg ccctgagtat aaccatcacc cagcgtcagc cgggaacgta acggaattat
     9481 gtctcgctca agccaggtat tgatatgctg ccatttattt ttgctacctg atgaactgtc
     9541 gctactgtta taactccagg tggtattgtc gcgtaaacgc cacgcaccaa tatttaaccc
     9601 actctgtagg tttaaatatg cataatggct gttaccccca attcgattct gtacactatt
     9661 tccgctgaaa ttataattga gcaatccggc attaataccg ggatcccata actcaggagg
     9721 aatataacca cgcgcgcgat tactcataaa tgcctgaggg atcgtcaggt tgagtcgctg
     9781 ctgaccaaca tctaaatgcg cagtagcgtc atggatcatt gaggttaatg gcacgcaggc
     9841 atcatccgcc agcagattca taccggagac agaagccgta ttcagcccca tactggcaag
     9901 ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg tcgcccgtgt taaatgtgac
     9961 atcacgcgtt gccatataac cattattcaa atagatatcg acgcgatacg tccctggcgg
    10021 taattcttgc ccattttcaa aacgcgataa atcggccaca gcctggggat catccgctaa
    10081 aaagcgcgga ttaaaataga gttcggcaga tgacaaaggt gcctgtgcgg caaaagcaca
    10141 ggcgacaaag agccggacaa aaaaaccagc caaacgatgc ttacgaatat gcaagcattg
    10201 tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt attcctccgg tttaaaatca
    10261 cccggcagtc aattcttttt attcaggcga aaaattcccc ctgcgttatt ccattacgcc
    10321 cgtcattttg ggggtaagtg cgccataatc atttattgtt cggtaagtaa tattgcttcc
    10381 tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga ggcaccaatg cattttcaag
    10441 aacccgggtt ccggcattca actctgttac cgtcaggtaa tagggtgtcg ggttaatcag
    10501 cgtcagagaa ttcgcgctac gacgaaatct taatttttct gcggcctgat cgggtggcaa
    10561 cgctaattta gccgggcgat agtacagctt aatgcggctg ataattgcga gctgtagcgt
    10621 attctcagtc aattttgatt tatccattga cggaatcgct ttaacgttca tccagaataa
    10681 actttcccga tcctgtggca attggttatt tgttgcatca agaatacgta aggtattctc
    10741 tctttttccc ttcatcgcaa acagaggtgg cgtcacgata aaacgaccat cctttacacc
    10801 atcggcattt tccacccatg attgaattaa ataggtgcta ttttcatcat tatttgtcac
    10861 ggcaagttgc acttgttttt gccctgccgg ataaattacg cgggtcgcac ctaaggccac
    10921 tcccgcttca gcgcgtccgg caaccatcat tgccatgaac atcaggatac ctgccagcaa
    10981 gcagaatgtt atttcctgcg atttccttac attgacgttt ttattactca ctgtcctgtt
    11041 cctgttatca cattatctgc tgaacaatta ctgataggtt aaagagaacc aggcctgggc
    11101 attggcgatg ccgccagtaa cccgacgccc ggtagcacga tatttggcga tgaaatgtag
    11161 cgaagtagag cctgagtaaa gccgtttcca gtttgctgga ggacgattaa tcggtacgag
    11221 gtttccttca tcatcaaata acgctacgcc aatattggtg gctatccctg gcccctctcc
    11281 cacggaaagc acatccggat ttttaccatc cgcgacaccg tgaaacgcca cacccacacg
    11341 ttcactcacc accgtgctac attcccgtaa atgaataaca aaaggcaccg gtgcgctatc
    11401 ttcccctacc gcatgaaacc ggttactgct gattttcccc atattgaccg tcatttgttt
    11461 atcaccagct tcaactcggc aagtttccgc aataatgacg ccctgaaatt gcatatttcc
    11521 gccgggcagc gtggtattcc atttatttcc ggccagagca aacattggca gcaacgacgc
    11581 taatagaaat aatctcttcc ttttcatcgt tatttttatc gcacaagggt gggcatccct
    11641 gcccgtaatg acgtccctga acctgggtag gttattgata ctgaaccttg aaggtcgcat
    11701 ccgcattagc agcacccggg gttgcctcgc cgattgcata ataacgcgcc tggaacggaa
    11761 tggtgttggt accgttattc aaggttgttt gtgcactaaa tgtcgcaccg tccagcgcca
    11821 gcgcagcacc cgttctgtcc aggatctgca caccaacttt tgttgcgcta cccgcagctg
    11881 aactctgcaa agctaatacg ttaggatgag ttccatcaat tgcggtacca aggaaggcaa
    11941 tagcagcttt gtctgcaaca gtagtgtcac aatcgttcag ttgaatgtta aaaccgacag
    12001 cagaactggt tgctccttct tgtgccagcg atgctgtacg aacctgccct aactgaacag
    12061 tttgatcaac agagcctgca tcaactgcgc aagcggcgtt aacaacctcc cctttaaagt
    12121 gaacggtccc accatttacc gtcgtgggtg tagtttcagc cagagccgct gtagaactca
    12181 gggacagagc cgacagaaca acaattgcca gagttttaat tttcatactg ctttcctttc
    12241 aaaaaactat ttctaaatcg acatgggcag tcgttctgta cactttgttt tgtcaacgag
    12301 tttgactgcc aacactgcac agttttcccc caaaagatga aacatttggg gccattttga
    12361 ctcatagagg aaagcatcgc gcacaaactt tttcagttta tttgttggct taatattcta
    12421 ttgttatctt tatttataga tttttatatt gcatgaggtg gtttttgtag agaagaatga
    12481 ggaagatgcg tcgagccaca gaaacgttag ctttacattt ggcggaggtg atgtgaaatt
    12541 aatttacaag agaaataatt tacatatcaa acagttagat gctttttatc gttttttaat
    12601 atttttatgc ttgagaaaaa atacgtaact tatttatgat atggacagtt tggccccaat
    12661 tgtcttgtat ttatttgttt tttaacttta ttatcaatga gttaaatcaa acctcttctc
    12721 tttttaattt ttcgtttatg agattatttc tttcccataa tccggcaaaa cgagcagcat
    12781 tactggcggt ataacgtaca gtatggcgaa tatttcgatg cccgagataa tcctgaatta
    12841 aacgggtatc ggcaccacgc tccgccagtt cataaccgca agcatgcctt aacatatgag
    12901 gatgagtctg cgttacggtt ccagcttcaa tacctgcatc gcgaataatg cgataggcct
    12961 gctggcgaga aagccgactc ccgcggcgag aaataaatat agcgtcggtc cggtcagcgc
    13021 ctttccagtt agcacgttcc tgggtccagc gttccacggc ttcacgctca tcaaaacgta
    13081 acgggtgaac ggtagaaaat ccgttcttca gtcggcgaat atttattcga ccttcattaa
    13141 ggtcaagatc ctgataatgc agatcaagca gttcactaat acgcatccca tgccgatatg
    13201 ccaacagaat aagacaataa tctctggctc ccgttgcccc gtaacaaacc gcctgcatca
    13261 tggcctgaac ttctttaccg gtaagataac gacgtttact cacaatagta gtactcctaa
    13321 ctgagatata tttgaatgaa tacctatagg aaacctcaat cggtcaaaat tagcccaata
    13381 gataaaaaga taaccacaca cgaagtgatg tggctatcag tcaattacat taaatatcat
    13441 acaaaaataa aatatcactg atgtgacaac atctattttc attgatttga tt
//
LOCUS       DAZGAP010000150        13351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGAP010000150 DAZGAP010000000
VERSION     DAZGAP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.151"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(480..1052)
                     /gene="hdeD"
                     /locus_tag="ACHLAM_003866"
     CDS             complement(480..1052)
                     /gene="hdeD"
                     /locus_tag="ACHLAM_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO7855324.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            1307..1639
                     /gene="hdeA"
                     /locus_tag="ACHLAM_003867"
     CDS             1307..1639
                     /gene="hdeA"
                     /locus_tag="ACHLAM_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO7855325.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            1755..2081
                     /gene="hdeB"
                     /locus_tag="ACHLAM_003868"
     CDS             1755..2081
                     /gene="hdeB"
                     /locus_tag="ACHLAM_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO7855326.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2145..2792
                     /locus_tag="ACHLAM_003869"
     CDS             2145..2792
                     /locus_tag="ACHLAM_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO7855327.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2844..3614)
                     /locus_tag="ACHLAM_003870"
     CDS             complement(2844..3614)
                     /locus_tag="ACHLAM_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO7855328.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(3611..4603)
                     /gene="shuU"
                     /locus_tag="ACHLAM_003871"
                     /gene_synonym="chuU"
     CDS             complement(3611..4603)
                     /gene="shuU"
                     /locus_tag="ACHLAM_003871"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO7855329.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4652..5275)
                     /gene="chuY"
                     /locus_tag="ACHLAM_003872"
     CDS             complement(4652..5275)
                     /gene="chuY"
                     /locus_tag="ACHLAM_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO7855330.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFINRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5275..5769)
                     /gene="hutX"
                     /locus_tag="ACHLAM_003873"
     CDS             complement(5275..5769)
                     /gene="hutX"
                     /locus_tag="ACHLAM_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO7855331.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(5782..7119)
                     /gene="hutW"
                     /locus_tag="ACHLAM_003874"
     CDS             complement(5782..7119)
                     /gene="hutW"
                     /locus_tag="ACHLAM_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO7855332.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLTQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(7139..8053)
                     /gene="shuT"
                     /locus_tag="ACHLAM_003875"
     CDS             complement(7139..8053)
                     /gene="shuT"
                     /locus_tag="ACHLAM_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO7855333.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTATERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            8737..10719
                     /gene="chuA"
                     /locus_tag="ACHLAM_003876"
                     /gene_synonym="shuA"
     CDS             8737..10719
                     /gene="chuA"
                     /locus_tag="ACHLAM_003876"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO7855334.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            10768..11796
                     /gene="chuS"
                     /locus_tag="ACHLAM_003877"
     CDS             10768..11796
                     /gene="chuS"
                     /locus_tag="ACHLAM_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO7855335.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(11857..12387)
                     /locus_tag="ACHLAM_003878"
     CDS             complement(11857..12387)
                     /locus_tag="ACHLAM_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7855336.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12540..13106)
                     /gene="slp"
                     /locus_tag="ACHLAM_003879"
     CDS             complement(12540..13106)
                     /gene="slp"
                     /locus_tag="ACHLAM_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO7855337.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3576 a         3099 c         3227 g         3449 t
ORIGIN      
        1 atttaaatat cgagtctcct tgtttcgact taggctggca attggattgc cagctttctt
       61 ttaacgcatt gaaattacct gattaaataa ctcattcatg tcttcaggtt tttatatagc
      121 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
      181 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
      241 cagcactttt aaaacattaa acctgaccac ggaaaatatc agccatgata atgtttgaaa
      301 tggctaattt gccatggaac gaaaaaaatc agattaaatt cagtaggttg aaataatcaa
      361 cagcaggcaa ttgtttcaat gatagtgcgt aattgaactg aaacactgta tagctaagag
      421 agtattaact ggatcatagt cacttaatgt gactatgatc caggtggaaa tagggataat
      481 tattgctttt taacgaacaa actggcgaag ctgaacagac tggcggcgct aaatatcagt
      541 tcaattccca ccagtgtgga aaccagcgtt accgacacca tcggcgtagc accaaggaat
      601 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccaact gcctttcatt
      661 gaacgctgac gataccaact catcaggcga ataacccctg ccacacagaa caaaccggca
      721 ataaatgccg caatggcaaa aatgcccagc tctggtgcgc ggatgaagaa atagccgatc
      781 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
      841 ttgctgaata gcccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
      901 gtgcttaaaa tgtcgccaga gacgaacgga aaactgatac acagcaaccc gacgataaac
      961 agcagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     1021 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
     1081 atcccctgct atcaatctat gccaaaaacg cgtgtaagaa tgcagtcgat ttaataaaaa
     1141 tttcctaatt acagtatctg atgcgtctgt aactcattgt attgaaataa aaatatctga
     1201 ttttgatatt ttccatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
     1261 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
     1321 aggcgttatt cttggtggtc tgcttcttct gccagtagtg agcaatgcag cggatgcaca
     1381 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
     1441 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcactgaaca acaaagataa
     1501 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
     1561 ggcttgtact caggataaaa aagccagctt taaagataaa gttaaaggcg aatgggacaa
     1621 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tatttccttg catattgaac
     1681 aggccggaat atcttcttta aaagcagcta ttcctcctgt ttgtatataa tctctatatt
     1741 gaatgggtta caaaatgaac atttcatctc tccgtaaagc gtttattttt atgggcgctg
     1801 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
     1861 atatgacctg ccaggaattt attgatctga atccaaaagc aatgacacca gttgcgtggt
     1921 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
     1981 atctcactca aattcctaaa gtgatcgaat actgcaagaa aaacccgcag aaaaatttgt
     2041 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
     2101 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
     2161 ttatacgact tatacttgcg gtaattgcct gtggcgctat tggcatggaa aggcaaatgc
     2221 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
     2281 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt aggtcacgtc ggactggacc
     2341 ccagccgtat tgctgctcag gttgtgacgg gcgtcgggtt tatcggtgca ggtaacattc
     2401 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
     2461 ccattggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
     2521 ccttgctggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
     2581 atttacagct gacattagtg aacggtaata cggtatcaat gctcgactgg tttaagcagc
     2641 aaaaaataaa aacggattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
     2701 ttgatatcca gcttcatgcc acgacgtcga tagaagatct gttgcgatta ttaaaaggta
     2761 tggcaggggt gaaaggcgtt tcaattagct aatctgtata gttatttatt cgcaggagta
     2821 ccagtgcggt actcctgttg gttttaatga tcgaggacga tcaggggtga atggttggca
     2881 ggatctttaa gtacggtaat atccgcgccg taaagcatgg tcagcgcctg ctgtgtcaat
     2941 acgtcctgtg gtttaccatt tgcgataacc tttccttttt gcattaatac aatacggtct
     3001 gcataacgcg cggcaagatt tagatcgtgc aatacgcagc aaacattgaa ctgccgttcg
     3061 tgaactaact ggcgtaataa acggaacaag tgctgctgat ggtgaatatc cagcgccgaa
     3121 gttggttcat caagaaataa ccatttcggt gagggagttg gttcccagag ttgcaccagc
     3181 aggcgcgcta attgtacgcg ctgttgttca ccaccggata attgccgata gtcgcggttt
     3241 gctaacgcct gacagtcaca taacgccatt atttgtgctg tttcattatc ctgattacca
     3301 gtgcgatgcg gatgccgccc catctggata acttcttgta cgctgaatgg aaacgccatg
     3361 tggctatttt gacgcatcac ggcccggtgt ttcgccagtt cagtaatcga ccattcattt
     3421 aatggcttat taaacaaccg acattcgccg ctatcaggtt gaagatagcc ggttaactgg
     3481 cgaagcagcg tagattttcc cgcaccatta gggccgagaa tggcaacaat ttccccaccg
     3541 ggaaaagtca gcgagacatt atcagtcaga cgccgcccct gaaggctata aaccagattc
     3601 tgggcgctga tcatgtgatc ctccggttgc gcagaatcaa ccacataaaa taaggaccac
     3661 caagcaggct ggttaataat cccacgggca tttctgctgg ctgtaccagc gtgcgggcaa
     3721 gcgtgtctgc catcagcaat aaacaggctc cggcgagggc ggagcaaggg attagccagc
     3781 ggtgatttgc tccggtagtc atgcgaatca gatgcgggat caccaggcca ataaagccga
     3841 taatgccgct taccgatacg gcggcaccaa cgagcagtga gctcactaac agtaattgct
     3901 ggcgtttacg cttaacgttc acgccgaggt aatgggcttc ttcatcaccg agctgcagta
     3961 aattcagcgt tccggcgaga caagttgttg cgataatggc cggtaggatg aatgaactgg
     4021 caaccaataa cgtcggccat tgcgcctgtc caagattgcc catcatccac aatgtgagtt
     4081 gtcgtagctg ctgctcatcg ccgatatagc tcaggatgcc gatcgctgcg ccgcacaggg
     4141 cattgatggc gataccggcg agtaataatt gcatcatgct gccatcgcga tggcgctgcg
     4201 tgatgagaaa aatgatggtg cagaccacta aacttccggc aatggcaaac accatttgct
     4261 catagagcac cagcaccact gggaaggaga agggcagcac aatagcaacg ccaaccatta
     4321 atgcagaacc gctactgacg ccaagtaatc cagggtcagc cattgggttg cgaaaaagcc
     4381 cctgcataat taccccggcg gttgccaacg ctgcacctac cagcaccgcc agcaaaaccc
     4441 gtggtagacg aatattgagc cagatatcgc ggtattcctc atcaaaaagg gcatcaaaac
     4501 tgatctttaa cgctccctga ctggcaccaa acagcaccag agctagcaat aatagtgata
     4561 gccccataaa gaccctggcg gaagagagtg aatccttgag cacgcaaaac tccgttacga
     4621 cattacggcg cggacatgcc gcgccggggg atcacgccgg tttcaggtcc ggttcaatca
     4681 gactgtagac ctgctggtta agtgctggcg catttgccag ttcatggata tgtgcggcga
     4741 catcagcacg gttaataaaa ccatgacact cctgattttg tatccgttgt gctttgcccg
     4801 ttgccgcacc atcaagcaga ccgccagggc ggagaatggc ataatcaagc tggctggttt
     4861 gcaaccagct ttccgccagc gttttttccc gtaccgcctg accaaacgca gcctttgccc
     4921 gctctgataa aaacggccag ctatcgccgc atcccagtga cgtcaccaga atcatgcgtg
     4981 tgatgcctgc tttctcggcc tcgtcaatca ctgttctgtg cgccagataa tcctgagctc
     5041 cgcccattgt tgagatgata agcgcatccg ggccagctgc gcggcaagca gccgcgacta
     5101 cgctggcatc acaggcgtcg cctgtaaaaa cctgtacgcc ttgttgcgcc agtttcgtgg
     5161 cggcatcggc atgacgaatg acagcaacga ccggacgttg ttccgccagc gccagttcaa
     5221 gcgttctggc acccacacct tttccgcctg cgccaaagag tagccacggt gtcatcaggc
     5281 gtgttccttt aacgatgctg ccagagcatg gaaggcactg acttgttcgc tcaggagctg
     5341 acgatgatca tccctaccga ggaagatttt cagcattgcg ctgccttcct tattgaaaaa
     5401 gagaatagag gcggtatcca tgcccataaa tttacgttcg atcaaggcaa tatgcgtgca
     5461 gttttctgct ttgatatgcc cggacatgcc gtgctttccg cgtaggttga aatagccgtg
     5521 gcgatggaaa cccgaaggca gttcgccgct aaattcgagt attacatcgg tagtatgtac
     5581 taacgtggtg acgttgcccc attcgcagac ggtatcccag acagtatcga atttatcacc
     5641 tgacacaaca gtcggagagg gcagattttt cactacctcc agcagcgtgg tgttgtattg
     5701 ttcggctatc gcctccagcg taccgtctgg ctccgttttc aaaaattcct gtaacgagac
     5761 atggctcata aatcactcct gttatggttt ttcaagcgca attgctggcg cattaagtac
     5821 ctgaatcagt tcattaagag actgcaaaat gttgcttgcc cagaaacgac cttcattggt
     5881 caaccgcaga cacgttgacg catcgcggct taagcctttt tggtgccact gggccagtaa
     5941 cggcgcgagt ttttcggcat gtggcgtaag ttcgtccagc ggtacacgcg ccgtttcaat
     6001 gcccgactgc aaggtatgac gccactgcgc gttacgttcg gcgttacgca tgatcatcat
     6061 cagcggtttt ttacctgctg cgacggattc atgccaggtc tgaagattgc gttcgttcat
     6121 ccaggagtaa ccattaatcg aaccgccggc tccggaacca aaggccagac aatcggcacc
     6181 ttgtttaatc agcaggttat agagattgcg ttcgcgtgtg gtacggcccc agtggctgtt
     6241 actgatgcag cgccaaccgg catcgtccat aaaatcacac ccttgcaggt aaagatcgcg
     6301 acgttctgcc ggagagggca cggtagtacg cccattttcc acggctttgc ccagcggtgt
     6361 attgggcagg acattgagcg catagagatc gacgccgtcg agaccgatat cgcgggcaat
     6421 agccagatct tctccccagg tttgcgcatc ctgacccggc agaccaaaca gcaggtcaca
     6481 gaccaccgcg gcacggtcgc gtttgaccag gctttccata aacgcaatag cggttgggcc
     6541 atccgaggtg cgggccattt tcttgcggat tttgctgtta aacgactgaa taccaattga
     6601 gaagcggttt gctccagcat caaggcaagc atcgattcgc tcagcgtcaa aattcagtac
     6661 ccggccttca atagtgattt cacaatcagg ggccagtggc agcttttctc gtaatgtggt
     6721 gatgattctg gccaaatcgt gtgctgaaag cgcggaaggc gtaccgccac cgaaatagac
     6781 cgcgtggatg ggggcagact gatgcaatac gctgtctgct tccatttcta tttcacgaat
     6841 cagggcgtcg gtataatgag cacacgcatc gtcattaaaa cggttctgat aaaaaccaca
     6901 aaacgtgcag tgtgtcgcac aaaaaggaat gtgtagataa accagtcgct tacgtggtga
     6961 taccgtttga ttgagcatct cttgccaggt ctgtgtcagt tgctctttgg caactggaat
     7021 ggccccacga aacggcatca tggcgcgcct gtctttaaat ggctgatcac cgtctaaagc
     7081 aaaatgtggc gtgagatcca gggtgttatt tgtgttcatt cgtctgattt aaatccattt
     7141 attgcggcca aagctgctgg tgtaaagact caacgacatc agcaatacgc ggacccattc
     7201 ccagaatcag gttttgatca acggtgataa tgcgctggtt tttccatgcg gcagtgtggg
     7261 taattccggc aatagaacgt agccgattaa tatcgccatc gaccatttgt gaggtgacga
     7321 caatcacttc agggttagcc gcaatcagcg attccgcgct gtagcttttg tactgctggt
     7381 gggtagcgac gttttctgct ccggcaagcg aaagaatggc gtccgcgacg ctgcctttac
     7441 cggcaacctg tggcgcactt ccgcctgcgg agagaataaa catggcttta accggggctt
     7501 ttttggccgc cacgttttgc tgtactcgct ccaggcgttg gttgatctgt gtcaccagcg
     7561 cctcgccttg ttcaggaacc tgtaacgttt ttgccagttg gcgaatgttg gcgtacatct
     7621 gctcaagggt ggctggtaca cgcggcagag tgacgacgtt gactttttgc gcccgcagtt
     7681 ggtcgagcac aatttgcggt cctgcatcct gccaggtaat tacgctatcc gggcgaagtg
     7741 acaaaatgcc ttcgctgctg agttgtttcc agtaaccaat atgcggcagt ttggcggttt
     7801 ctggtggata agatgtcgtt tcatcgacac caaccacgcg ttcgccagcg cccatcgcgt
     7861 agattagctc cgtcagcgat cctcctgcga ccacgatacg ttcggtagcc gttacgctaa
     7921 aagcccagct aagagccagt actgccgtaa acaatttttt tcgttttacc gcggtcttcg
     7981 cggcagaggc cacggctgaa atacataacg tcagtgggga aaaaagaaac ggcctggtga
     8041 tgatccttgg cataatatga gaactcttat caaattgaga atgatcatga ttatcatttg
     8101 agttatatat gaaatacaac cgtcgattca atacgcacgg cgttacaagc gtatttattt
     8161 ttgattttta tcaagatgct tcgtcagcta acgttcttgt taccgactaa atttcagcgc
     8221 gggaaaaagc tgagaaccca gagatatcgc ggcttgcaga gcaaagagaa gagaaaatta
     8281 tcactgttaa cgacaaagtt tagtgatgag gtcatggggg aataaccata aaaacaaagt
     8341 ggtttccagg gcgcaggata agtttcataa ccccctgttt attgtgttaa tggcggttaa
     8401 aaaaaagctt gatcataaaa aattgatcga taataattct cattatcacg tttgcattta
     8461 acaaaatacg gccatatgga taacaaagcc atatcattta caaggggtaa cgcacagcgc
     8521 tcttcactcc tgatggggca tatcggtttg cctgacaaag agggcgtgct tttgcgtatt
     8581 tgccgagata ccaggcaatt tcatgggata taaacgcctg taaactcacc cggcgaatat
     8641 tatcgctctg aattttaaat aaatttattt ttcgccagcc accccggtat taacccaggc
     8701 agccagcatt ttttattttc ctcatggaga atcgctatgt cacgtccgca atttacctcg
     8761 ttgcgtttga gtttgttggc tttggctgtt tctgccacct tgccaacgtt tgcttttgct
     8821 actgaaacca tgaccgttac ggcaacgggg aatgcccgta gttccttcga agcgcctatg
     8881 atggtcagcg tcatcgacac ttccgctcct gaaaatcaaa cggctacttc agccaccgat
     8941 ctgctgcgtc atgttcctgg aattactctt gatggtaccg gacgaaccaa cggtcaggat
     9001 gtaaatatgc gtggctatga tcatcgcggc gtgctggttc ttgtcgatgg tgttcgccag
     9061 ggaacggata ccggacacct gaatggcact tttctcgatc cggcgctgat caagcgtgtt
     9121 gagattgttc gcggaccttc agcattactg tatggcagtg gcgcgctggg tggagtgatc
     9181 tcctacgata cggtcgatgc aaaagattta ttgcaggaag gacaaagcag tggttttcgt
     9241 gtctttggta ctggcggcac gggggaccat agcctgggat taggcgcgag cgcgtttggg
     9301 cgaactgaaa atctggatgg tattgtggcc tggtccagtc gcgatcgggg tgatttacgc
     9361 cagagcaatg gtgaaaccgc gccgaatgac gaatccatta ataacatgct ggcgaaaggg
     9421 acctggcaaa ttgattcagc tcagtctctg agcggtttag tgcgttacta caacaacgac
     9481 gcgcgtgaac caaaaaatcc gcagaccgtt gaagcttctg atagcagcaa cccgatggtc
     9541 gatcgttcaa caattcaacg cgatgcgcag ctttcttata aactcgcccc gcagggtaac
     9601 gactggttaa atgcagatgc aaaaatttac tggtcggaag tccgtattaa tgcgcaaaac
     9661 acggggagtt ccggcgagta tcgtgaacag ataacaaaag gagcaaggct ggagaaccgt
     9721 tccactctat ttgccgacag tttcgcttct cacttactga catatggcgg tgagtattat
     9781 cgtcaggaac aacatccggg tggcgcgacg acgggcttcc cgcaagcaaa aatcgatttt
     9841 agctctggct ggctacaaga tgagatcacc ttacgcgatc tgccgattac cctgcttggc
     9901 ggaacccgct atgacagtta tcgcggtagc agcgacggct acaaagatgt tgatgccgac
     9961 aaatggtcat ctcgcgcggg gatgactatc aacccgacta actggctgat gttatttggc
    10021 tcatatgctc aggcattccg cgccccgacg atgggcgaaa tgtataacga ttctaagcac
    10081 ttctcgattg gtcgcttcta taccaactat tgggtgccaa acccgaactt acgtccggaa
    10141 actaacgaaa ctcaggagta cggttttggg ctgcgttttg atgacctgat gttgtccaat
    10201 gatgctctgg aatttaaagc cagctacttt gataccaaag cgaaggatta catctccacg
    10261 accgtcgatt tcgcggcggc gacaactatg tcgtataacg tcccgaacgc caaaatctgg
    10321 ggttgggatg tgatgacgaa atataccact gatctgttta gccttgatgt ggcctataac
    10381 cgtacccgcg gcaaagacac cgataccggc gaatatatct ccagtattaa cccggatacc
    10441 gttaccagca ccctgaatat tccgatcgct cacagtggct tctctgttgg gtgggttggt
    10501 acgtttgccg atcgctcaac acatatcagc agcagttaca gcaaacaacc aggctatggc
    10561 gtgaatgatt tctacgtcag ttatcaagga caacaggcgc tcaaaggtat gaccactact
    10621 ttggtgttgg gtaacgcttt cgacaaagag tactggtcgc cacaaggcat cccacaggat
    10681 ggtcgtaatg gaaaaatttt cgtgagttat caatggtaat catctgcccc gatatttcgg
    10741 ggcatttatc tggaaggaag agagacaatg aaccactaca cacgctggct tgagttaaaa
    10801 gaacaaaatc ccggaaagta cgcgcgtgac atcgcaggtt taatgaatat tagcgaagca
    10861 gaactggcat ttgcgcgcgt cacgcacgat gcgtggcgga tgcgcggcga tatccgtgaa
    10921 attctggcgg cgctcgaaag tgttggcgag accaaatgta tttgtcgtaa tgaatatgca
    10981 gtccatgagc aagttggtgc gttcacaaac cagcatttga acggacatgc cggattgatc
    11041 ctcaatccac gcgcgctgga tttgcgtctg tttctcaatc aatgggccag tgtttttcac
    11101 attaaagaaa atacgactcg tggcgaacgc cagagtattc agttctttga tcatcagggc
    11161 gatgcattac taaaagttta tgccaccgac aataccgata tggcggcatg gagtgagctt
    11221 ctggcacggt ttatcgccga tgagaatatg ccgcttgagt taaaagccgt tgatgcgcca
    11281 gttgttcaaa cgcgagccga tgccagtgtg gtcgagcaag agtggcgggc gatgaccgac
    11341 gttcatcagt tttttacgtt gctcaagcgc cacaacctga cgcgccaaca ggcgttcaat
    11401 ctggtggcag atgatttggc ctgcaaagta tccaacagtg cgttggcgca aattcttgaa
    11461 tctgcacagc aggatggcaa tgaaatcatg gtgtttgttg gcaaccgtgg ctgcgtacag
    11521 attttcactg gtgtggtaga aaaagtggtg ccaatgaaag gttggctgaa tattttcaac
    11581 ccgacgttta ctcttcatct attagaagag agcattgctg aagcctgggt tacccgtaaa
    11641 ccgaccagcg atggctacgt aaccagtctg gaattgtttg cccatgatgg tacgcagata
    11701 gcgcaacttt atggtcaacg tacagaaggc gaacaggagc aagcgcaatg gcgtaagcaa
    11761 attgcttcgc tgataccgga agtcgttact gcataacgta taattaacgg gaggtctggc
    11821 tatttattgc tgggcctctt tatattgatc aatcgttcac accagataat caatatgctg
    11881 atggcgtacc aactcattga tccgcttaag attgaggatc atcatgatat tgtgtttatg
    11941 gcagtagaac gtttttagcg atattttcag tttctccaga atatcttccg ttgtcatttg
    12001 attcgcaatg cagctcagaa cctgatgctg cttgcgcgaa agggacacat ccgcttcagg
    12061 cacagagcag taactgccat tgatgagtgc gacaaagtcg atgatggtga tagttcgcgg
    12121 aacaaaggtc accggtactt tccccaggca gcgtttaata ttaaagggag agaaaataat
    12181 cacatggaca ggttttaatc gttcaatctg agtgagcaaa ttagaatgaa atacattatt
    12241 cattaatgta tcaataatga cgaaggcatg ctgatgcaac attacgtcga tctcttcttc
    12301 gttctgtata tgaacgacat tacctttact cagaatgttt ttcatcgcag tgaagaacat
    12361 cgtatccctg gtaattataa gaaacataac cgactcctgg tgcggacatt aactttataa
    12421 tcagtttgcc aaaggtaatt aacaggacag ggcttataat aatagttagc tggctgtgta
    12481 ttaatttgat gtaatgcatg atgcagatgc tgagaaagca tctgcatctc tcggtggtgt
    12541 tatttgacca gctcaggtgt tacttgactc accgcattgg tgtagtaagg cgcgccccag
    12601 cccggttccg gccagaatgc accatagccg taatcccaca ggttataggt ggtattaacc
    12661 acttctctca aatgccacac ctggatgccc tgcatattca cttccaggaa gttatacggg
    12721 actttattga taaagccagg ttgttcaccc tgaatggtac cgaggatggt aacaaagtga
    12781 ttacgatagt tcactggatc aaggaagccg ctttgtctgg cgagcagtcg gccctgatag
    12841 ttggcttcaa tatcaggctt cgcatagcta tccaacggta atacagcgat ttctaacaac
    12901 gtatccgttt tgccattgat aacgttgata accttcccgc caaagcgcgc ttgttgacca
    12961 acatataacc ccggctggtt atgaacagca acaaaacttt tttgaatatc aggttggtta
    13021 ttgcctttga tattttgcgg aattgaacta catgcggcaa gcaaaaatga aaggctgagg
    13081 atgagtgcac cttttgtcat gttcatgtta ctatccttat caacaaatat taaaaccata
    13141 atagatattt ctttatagtt ttcatctgat tctgaggcga tatgcctgat agtaacgtaa
    13201 acctgtctga tgtttatatc ccttctgttt agtttaattc tgttaatttt ggggcgtgac
    13261 atgagtatcc atcgccccat aaaatagcct ttttttaaag gtacataaaa tctattttta
    13321 ttcaatgatt ataaagtgct aatgttcaat a
//
LOCUS       DAZGAP010000151        13348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGAP010000151 DAZGAP010000000
VERSION     DAZGAP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.152"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..723
                     /gene="ais"
                     /locus_tag="ACHLAM_003880"
     CDS             121..723
                     /gene="ais"
                     /locus_tag="ACHLAM_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO7855338.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(762..1187)
                     /gene="nudI"
                     /locus_tag="ACHLAM_003881"
     CDS             complement(762..1187)
                     /gene="nudI"
                     /locus_tag="ACHLAM_003881"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO7855339.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            1466..2008
                     /gene="yfaZ"
                     /locus_tag="ACHLAM_003882"
     CDS             1466..2008
                     /gene="yfaZ"
                     /locus_tag="ACHLAM_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO7855340.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2108..3310
                     /gene="yfaY"
                     /locus_tag="ACHLAM_003883"
     CDS             2108..3310
                     /gene="yfaY"
                     /locus_tag="ACHLAM_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO7855341.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQTRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3530..4312
                     /gene="yfaX"
                     /locus_tag="ACHLAM_003884"
     CDS             3530..4312
                     /gene="yfaX"
                     /locus_tag="ACHLAM_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO7855342.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4327..5532
                     /gene="rhmD"
                     /locus_tag="ACHLAM_003885"
     CDS             4327..5532
                     /gene="rhmD"
                     /locus_tag="ACHLAM_003885"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO7855343.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VISFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYNH"
     gene            5589..6878
                     /gene="yfaV"
                     /locus_tag="ACHLAM_003886"
     CDS             5589..6878
                     /gene="yfaV"
                     /locus_tag="ACHLAM_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7855344.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6895..7698
                     /gene="yfaU"
                     /locus_tag="ACHLAM_003887"
     CDS             6895..7698
                     /gene="yfaU"
                     /locus_tag="ACHLAM_003887"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO7855345.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAADKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(7739..7972)
                     /locus_tag="ACHLAM_003888"
                     /pseudo
     CDS             complement(7739..7972)
                     /locus_tag="ACHLAM_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7973..8968)
                     /gene="rpnE"
                     /locus_tag="ACHLAM_003889"
     CDS             complement(7973..8968)
                     /gene="rpnE"
                     /locus_tag="ACHLAM_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO7855346.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDSEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKIEGWQ
                     EGKLEGLQEGKLDGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(9161..10351)
                     /gene="glpC"
                     /locus_tag="ACHLAM_003890"
     CDS             complement(9161..10351)
                     /gene="glpC"
                     /locus_tag="ACHLAM_003890"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO7855347.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10348..11607)
                     /gene="glpB"
                     /locus_tag="ACHLAM_003891"
     CDS             complement(10348..11607)
                     /gene="glpB"
                     /locus_tag="ACHLAM_003891"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO7855348.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLEALRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQL"
     gene            complement(11597..13225)
                     /gene="glpA"
                     /locus_tag="ACHLAM_003892"
     CDS             complement(11597..13225)
                     /gene="glpA"
                     /locus_tag="ACHLAM_003892"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO7855349.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQRIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTMSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
BASE COUNT         3048 a         3387 c         3576 g         3337 t
ORIGIN      
        1 tgaggcattt tgccggcatc gtaggatttt taatattaca ccaactgcta attatcgcca
       61 gaaatgtcgc tcaatttcac ggtaattgtc tggttgcgct tgtctatagg tggagtttac
      121 gtgttagctt tttgccgctc ttcgttgaag tcaaaaaaat atttcatcat tttactggcg
      181 ctcgctgcaa ttgccggact gggtactcat gccgcctgga gtagcaatgg tttgccacgt
      241 atcgacaata aaacgctggc cagactggca cagcagcatc cggttgtcgt tttgtttcgc
      301 catgctgaac gttgcgatcg ttcaaccaat caatgcttgt cagataaaac aggtattacg
      361 gtcaaaggta cccaggatgc ccgtgaactg ggcaacgctt ttagtgctga tatccctgat
      421 ttcgatctct attccagtaa taccgtccgg accattcagt cggcgacctg gttttcagcg
      481 ggtaaaaagc tgacggtaga taaacgactt cttcaatgcg gtaatgagat ttatagtgca
      541 attaaagact tacaacgcaa agcgcctgat aaaaatatcg ttatttttac ccataatcat
      601 tgcctgacat atattgctaa agataagcgt gatgcgacat ttaaacctga ttatctggat
      661 ggtttagtca tgcatgtgga aaaaggcaaa gtttatctgg atggggaatt cgttaaccac
      721 taaattttca atctgacagc cagtgatggc tgtcatcatt attacagaag acctttcaaa
      781 cgtaacgttt ttcgggtggc gacattcaaa tcatagtgcg ccagatcttc aggttttacc
      841 cacgcgtagt cttgaaactc ttcgtttatt ttcacttctc ggttggcaga aacgcagtca
      901 aaaatcaggt aaatcatata aatctcttcc ttgcgaccat ctgcatacgt cttggtgcga
      961 atatcatcgc tgaaggtcca cggcgtgatt tctgtcaaaa gcagctgttc tcccagctct
     1021 tcgcgaattt cgcggcgtag tgcctcttca attcgttcgc cgggctccac gccgccaccc
     1081 gaaagcgccc attgaccggg gaaaacgccg cgatcgtcgg ccattttaca cagcaaataa
     1141 gcaccatcat tttgaatcaa agggcataca atagtccgtt gtcgcacagg ggtattcctt
     1201 ataaataaaa agaaaccatg agaatacggt aactcagaaa tatgtgaaag tgacagtaat
     1261 ctcttttgcg atgataactg cacttattat tatttttgtg tgtatcggta aactgtgttt
     1321 attttttcgg cgtttaatta tttaagaagt atttagttac aataagtgac tagatccctt
     1381 ttttcgctca ttgaatcctc tgcgccaggc agtacaatcc cgcagctttg ggaattttga
     1441 ttaattgctt gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc tggccggaat
     1501 gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc ggccaggcgg gtaaagaata
     1561 caccaatatt ggtgtcggtt ttgggactga atcgacgggc ctggcgttaa gcggtaactg
     1621 gacacataac gacgacgacg gtgacgtcgc gggcgtgggg ctggggttga atctgcctct
     1681 cgggccgtta atggcgaccg ttggcggaaa aggcgtttac accaacccga attacggcga
     1741 tgaaggttat gccgcagcgg taggaggtgg tttgcagtgg aaaattggca acagcttccg
     1801 cttgtttggc gagtattact actctccgga ttcgctctcc agcggtattc aaagttatga
     1861 ggaagcgaat gctggcgcgc gttacaccat tatgcgtcca gtcagtattg aggcgggtta
     1921 tcgctacctg aacctttctg gtaaagacgg taaccgcgac aacgcagtgg ctgacggccc
     1981 gtatgtgggg gttaacgcca gtttttgatc ccttccggcg cggcattctg ccgcgccatt
     2041 cagtttctta ctacgcctta tctccctttg cgttatagtg attcgataac aaaagcagga
     2101 gataaccatg ttaaaagtgg aaatgttatc caccggggat gaagtgttac acgggcaaat
     2161 cgttgacact aacgctgcct ggctggccga ttttttcttt catcaggggt tgcctttaac
     2221 tcgccgcaat acggtggggg ataaccttga tgacttagtg accattcttc gcgaacggag
     2281 tcagcacgcc gatgtgctga tcgttaacgg cgggctgggg ccgaccagtg atgatttaag
     2341 cgcactcgcc gctgcgacag caaaaggtga aggcctggtg ctgcatgagg cctggctcaa
     2401 agagatggaa cgctatttcc acgaacgtgg acgagtaatg gcaccgagca accgtaaaca
     2461 agcggagctg cctgccagtg ctgaatttat caataacccg gtaggcaccg cctgtggttt
     2521 tgccgtgcag cttaatcgtt gcctgatgtt ctttaccccc ggcgtaccgt cagaatttaa
     2581 agtgatggtc gagcacgaaa tcctgccgcg cctgcgcgag cgtttttctt taccgcagcc
     2641 gccggtttgt ctgcgtttga ctacatttgg tcgttcggaa agcgatctgg cacaaagcct
     2701 ggacaccctg caactgccgc cgggcgtaac aatgggctat cgctcttcta tgccgattat
     2761 cgaactgaaa ctcaccggac cggcaagcga gaaacaggcg atggaaaaac tgtggctgga
     2821 cgttaaacgt gttgccggac agagcgtgat tttcgaaggt accgaaggac tgccctcgca
     2881 gatcagccgc gaattgcaaa cccgccagtt cagcctgacg ttgagcgagc aattcacagg
     2941 tggtttattg gctttgcaac tttctcgcgc aggtgctcca ttgctggcgt gtgaagtggt
     3001 tccttcacag gaggaaaccc tggcgcaaac tgcgcactgg attacagaac ggcgggccaa
     3061 ccattttgcc gggctggcac tggctgtttc gggtttcgag aacgagcatc tcaactttgc
     3121 gctagccacg ccagatggca ctttcgctct gcgtgtgcgt ttcagcacta cacgctacag
     3181 cctggctatc cgtcaggaag tgtgcgcaat gatggcactg aatatgctgc gccgttggtt
     3241 aaacggccag gacatcgcca gtgagcatgg ctggattgag gttgttgagt ccatgacctt
     3301 atctgtctga ttccgccact ctgtgccggt ttactgcacc ggcacattcc ccctccggca
     3361 aaatttgtcc aacctgttgt actcaataag aaaaaatccg tgcgctaatt cacaaatttg
     3421 tgcgtaattt ctccagtccg ctggacgagg agcgggattt gatgagcttc acaattttac
     3481 tccacctgag caacgagact gattccagta tctaagactg gagtccagta tgttggaatc
     3541 aagcaaagtt cccgccctga cacgggctat tgatattctc aatttgattg cccgcattgg
     3601 cccctgtagt gctgcgacga tcattgacac attggggatc cctaaaagca cggcctattt
     3661 gctgcttaat gaactcaggc gtcagcgttt tcttagtctt gatcaccagg aaaatttttg
     3721 tttgtggacc aggctggtgg agctttccgg tcatgcgtta agcaagatgg atctccggga
     3781 actggctcga ccgcgtttga cgcaactgat ggataccacc gggctgttat gtcacctggg
     3841 gattattgat aacggcagcg cttattacat tttgaaagtg gagtcatcag ccactatcag
     3901 cgtgcgttcc catgaaggaa aaagcctttc gctttatcgc tccggaatag gtaaatgcct
     3961 gcttgcctgg caacctgcag ctgtacagca aagtattatc gaagggctga tctgggagca
     4021 ggcgacaccc acaacgatta ctcatccaca gcaacttcac gaagaactgg cgcgtattcg
     4081 ccgtcagggc tggagctatg acaacggcga agattacgcc gatgtccgtt gtgtcgctgc
     4141 gccggtgttt aacgccaata acgagcttac cgctgcgatc tcggtggtcg gtacccgctt
     4201 acaaatcaat gaagaatacc gtgattatct ggcgggcaag gccattgcct gcgccaggga
     4261 tatttcacgt ctgttgggat ggaaaagtcc ctttgactta caggcctcat aacaatggag
     4321 aacagcatga ccctaccaaa aatcaaacag gttcgcgcct ggtttactgg cggtgcgaca
     4381 gcagaaaaag gcgctggcgg cggtgattat cacgaccagg gggcaaatca ctggattgat
     4441 gatcatattg ccaccccgat gagtaaatac cgcgattacg agcagtcacg ccagtcattt
     4501 ggcattaacg ttcttggcac gttgattgtt gaagtcgaag cagaaaacgg ccagaccgga
     4561 ttcgccgttt cgacagccgg tgaaatgggc tgttttattg tcgaaaaaca ccttaaccgt
     4621 ttcattgagg gtaaatgtgt cagtgatatc aaactgatcc acgatcaaat gctcaatgcc
     4681 accctgtatt actccggctc tggtggcctg gtgatgaaca cgatttcctg tgtcgatctg
     4741 gctctgtggg atctgttcgg caaagtggtc gggcttccgg tttataaact tttaggcggc
     4801 gcagttcgtg atgagattca gttctacgcc acaggtgcgc gtccggatct ggcaaaagag
     4861 atgggcttta tcggtggcaa aatgccgacg cactgggggc cacatgatgg cgatgcgggg
     4921 atccgcaaag atgccgctat ggtcgcggat atgcgtgaaa aatgcggtga ggatttctgg
     4981 ttaatgctcg actgctggat gagtcaggac gtgaactatg ccaccaaact ggcccacgct
     5041 tgcgcgccct ataacctgaa atggatcgaa gagtgcctgc cgccacagca gtatgaaggt
     5101 tatcgcgaac tgaaacgcaa cgcgccagtc ggaatgatgg tcaccagcgg tgagcatcac
     5161 ggcacactgc aatctttccg tacgctttca gaaaccggta tcgacattat gcagccggat
     5221 gttggctggt gcggtggctt aaccacgctg gtggaaattg ccgcaatcgc caaatccaga
     5281 gggcaactgg tggtgccgca cggttcgtct gtttactctc accatgcggt gatcagcttc
     5341 accaatacgc cattcagcga attcctgatg accagcccgg attgttcaac gatgcgtccg
     5401 caatttgatc cgattctgct taatgagcca gtgccggtga atggtcgtat tcataaatca
     5461 gtgcttgata aacccggttt cggcgtcgaa ctcaatcgtg actgcaattt gaaacgcccc
     5521 tacaaccact aatcactccg ggcgttgctg tgacaacggt aacgcccaca tattctaagg
     5581 agctttttat gagcaccgct ttgcttgacg ccgtggtgaa gaaaaaccgt gcgcgtttaa
     5641 ttccgtttat gttggcgctg tatgtgctgg cgtttctcga ccgttcgaat atcggttttg
     5701 ccaaacagac ctaccagatt gataccgggc tgagtaatga agcttatgcg ctgggagcag
     5761 gcattttctt tgtggtatat gcgtttctag gtgttccggc gaatcttttg atgcgcaaac
     5821 tgggggccag aacctggatt ggtacgacaa cactgctgtg gggatttctt tcggcagcca
     5881 tggcatgggc cgatactgaa gcgaaatttc tgatcgttcg cactctgctt ggtgctgcgg
     5941 aggccgggtt cttccctggt atgatttatc tcacctcgca atggtttccg cagcgtaatc
     6001 gcgccagcat tatggggctg ttctatatgg gcgcaccgtt ggcgttaaca ctgggatcac
     6061 cgctttccgg cgcgctgctg gagatgcatg gatttatggg acatcccggc tggttctgga
     6121 tgtttgtgat tgaaggattg ctggcagtcg gcgctggggt attcacattc ttttggcttg
     6181 atgacacacc ggagcaggca cgttttctga gtaaagaaga aaaaacattg cttatcaatc
     6241 aactggcaag tgaagaacaa cagaaagtga cttctcgact gagcgatgcg ctgcgtaatg
     6301 ggcgagtctg gcaactggcg attatctacc tgaccattca ggtggcggtt tacggattaa
     6361 ttttcttcct gccgacccag gttgctgcat tgctggggac aaaagtgggc tttacggcgt
     6421 cggtggtcac cgccattccg tgggttgcgg ccttgtttgg gacctggctt attccgcgct
     6481 attccgataa aaccggcgaa cggcgtaatg tcgcagcgct gacattactg gcggcaggca
     6541 ttggtattgg tctctccggg ctgctttctc cagtactggc gatcgtagcg ctgtgtgttg
     6601 cagccatcgg gtttattgcc gtgcagccgg tgttctggac gatgccgaca caactgcttt
     6661 ccggtacggc gctggcggcg gggattggtt ttgtaaacct gtttggtgca gtgggtgggt
     6721 ttattgcccc gatcctgcgc gtgaaagcag aaacgttgtt tgccagcgat gcggcgggat
     6781 tactgacgct ggcagcggtg gcggtcatcg gttcgctgat tattttcact ctgcgtgtaa
     6841 atcgcactgt tgcgcagacc gacgtggcac atcattaata ggttaaggaa cacgatgaac
     6901 gcattattaa ccaatccctt taaagaacgt ttacgcaagg gcgaagtgca aattgggctg
     6961 tggttaagct caacgactgc ctatatggca gaaattgccg ctacttccgg ttatgactgg
     7021 ttgctgattg acggggagca cgcgccaaac accattcagg atctttatca ccagctacag
     7081 gcggtagcgc cctatgccag ccaacccgtg atccgtccgg tggaaggcag taagtcgctg
     7141 attaaacaag tcctggatat tggcgcgcaa actctactga tcccgatggt cgatactgcc
     7201 gatcaggcac gtcaggtggt gtctgccacg cgctatcctc cctacggtga gcgtggtgtc
     7261 ggggccagtg tggcacgggc tgctcgctgg ggacgcattg agaattacat ggcgcacgtc
     7321 aacgattcgc tttgtctgtt ggtgcaggtg gaaagtaaaa cggcactgga taacctggac
     7381 gaaatcctcg acgtcgaagg gattgatggc gtgtttattg gacctgcgga tctttctgcg
     7441 tcgttgggct acccggataa cgccgggcac ccggaagtgc agcgaattat tgaaaccagc
     7501 attcggcgga tccgtgctgc ggataaagcg gctggttttc tggctgtggc tcctgatatg
     7561 gcgcagcaat gcctggcgtg gggagcgaac tttgtcgctg ttggcgttga cacgatgctc
     7621 tacagcgatg ccctggatca acgactggcg atgtttaaat caggcaaaaa tgggccacgc
     7681 ataaaaggta attattgata tcaaaggccc atgggggtcg gctgtgggcc tgtgttaatt
     7741 agtggttatt cgctgccaga tcggcttcgc ttagctgggt ggctgcgagc accaggtcac
     7801 gatcaatgcc ctgtaccagc atgcgcaaag caattttaat ggcttgttta cgcatacctt
     7861 cctgcaaacc ttctaattta ccctcctgcc agccttcaag cttaccttcc tgccagccaa
     7921 tctgatgccc ttcctgcctc agccgttcag caatagtcat caatctctcc ttttaatgac
     7981 agttcggaat atcagcatca gtgagttggg ttgtcgccag cacaatctca cgttcaaagc
     8041 cctgctccag catgcgcaag gcaattttaa tggcttgttc atgcatgcct tcctgcaaac
     8101 catctaattt accttcttgc aaaccttcta atttaccttc ctgccaacct tcaattttac
     8161 cttcctgcca gccttcaatc ttaccttcct gccagccaat ttgatgccct tcctgccgca
     8221 gccgttcagc aatagtcatt aatctctcct tttgtagtgg tgaacgttcg gcaatctcct
     8281 caataaaacg ggtgaaacgg gaggtatcgc cgcattgcag cagataatta aacagcgttt
     8341 gtagctggct gtcattagtg aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatac
     8401 cgattaaatc gcggtcgcga atatgctttt gaatcagttc aagcagagct atacgccgat
     8461 gttgcatgat ctcatcgtca ggtacgatgg tgatatccac caacggaaaa gcttcggtgt
     8521 acaactgccg ggcaagttgc ggatcgtcaa actcatccag ccagttgagt gagtacgggt
     8581 agggcgaggt ttcgccatga taaaacagca atggcaccac cagcggaact ctgtcatagc
     8641 ctttatccag gtgacgctgc atggcggcag tggcatagcg cattagccga aaagccatat
     8701 tcttttctgc agagctttga tgttcaatca cgcagtagat atagccttca ctgtcgctgg
     8761 tttccacaga ccacaaaaca tccgagtagt aagcgcgtaa acttttttca ataaagctgg
     8821 tgggttccag gcgtaaagtt tgcaggttgc aaagcttgcg cagcggctct ggtagatgta
     8881 tttccagaaa atcccgagcg gtttcgggtg tgaacataaa ggttttaaac accgcatcat
     8941 gcggggagga ggttgttgat tcggtcatgg ttgtccgtca cctgatattt atgatgacgc
     9001 gacattacgg ttatcccagg cctttgacat ctgccagttt cagcctttgc gagacgtttt
     9061 cagaggaatt ttcactgcgt tgcaacgcat acattgggca cggaatcgtg tacgcaaaaa
     9121 tgcccggtaa caacatgctt accgggcatc agaaaggagt ttaagccagc gcctgggcca
     9181 gcagcgtaat cggatgttcg cagcgaagac ttgtggacat ctcaatctgc catttgcagg
     9241 tttcgcagtc ggtaaccacc agatctgcgc cgctttcttc tatctgacgg aacagtggtg
     9301 cgccgatggc ttgtgaggtg gggtagttct cttttttgaa accgtaagta cctgcaatac
     9361 cgcagcactg ggaatccagc accgttaact caagccccgg gattttacgc aacagctcca
     9421 gggtgtagag cgtccagccc attttttcca tatggcacgg agtgtgataa accactttca
     9481 gcggcagcgg tttcagcggc aaggttttgc cttcgtccag cttgcgccac agccagcggg
     9541 tcgccagttc gatgtgatcg cgcaagcctt tgttgtcgac attcagcact tccggatatt
     9601 cgtcgcgcag ggcaaatgta caggttgagg aggtggcaat caccggaatg ccttttactc
     9661 ccactgcttc gcggatcgac tctacatttg tgattgcctg tttacgtgct ttatcggtaa
     9721 agccgttggc gatcagcggt acgccgcagc atttttcttt gctgagcagt tgtacaccgg
     9781 tacccattgc gttgagcact ttaattaaat ctttgcctaa ctgcggatgg ttgtagttaa
     9841 cgaagcagcc gtgaaagaaa gccacctggt ctttatattg tgcctgctgg gccgccacgc
     9901 tgcgatacca gcgacggaac gtaccgaagg agtatttcgg tagcgtgcgg cgatgatcga
     9961 tttttaacgc cgcatcaagc agctgccgca ctggtttcag cgaggtagcg gtattgacga
    10021 ttggtgcgaa tggcgtggaa acgctaccca tcaggtcggt atgactcaac acaaaattgc
    10081 gcagcgacgg gcgtgtggtg tcatatttcg cccgcgcgcg ctggatgata tcgccaatct
    10141 tcacatcgga cgggcaggcg acttcgcagc gtttgcagtt gatgcaatat ttcagcgctt
    10201 cgtcatacag tgcgccatct ttcagacgca gacgctcgcc atccggcccg gcttgttttg
    10261 gccctggata gccgggattc acccggctca ccgggcaggc ggtggtgcag acggtgcact
    10321 taatgcagtt ttcgaagctg gtgtcgttca tagttggcct cctgcgcgtt gggcaatctg
    10381 ttgagcggca tgtaaagcac tgacggcaca aacaccgccg ccgcatccct gggcgatggg
    10441 atcaaatccg cccagcaccg aaccgatggc aaacaggttc tcaatggttt gccctgcctg
    10501 cgatgggcgt agtgcctcat cagtggttac accgaacagc tgccacggtt gcggcgcaaa
    10561 aaaatcgccc ttataccatt caccccgcgt ggcggtttgt agcacatcaa ggccgagaat
    10621 cggctcgcga atgccgtcac gttctgccac cagtccgccg ctaaagaaac tgccgctcgc
    10681 aagcaccgca aatcgtgggc gcagcgggat atcggcgtga ttgcgcgtcc agatttcatt
    10741 cactacgcca tttttacagg tcactttttt cacttcatcg cctggcatcc acacgccacc
    10801 ctggcgcaca aactggcgct gtaactggtt ttgcagacga atgcccagca cggaaggcgg
    10861 cagcgttggc aaaagcatca gtgaacaagg tagtttttca ttcaaccaac gccacagttt
    10921 gtcatcggcc aggccgaagc aggcgggcat caggatcatt tcgcaggtat tggcgacagg
    10981 aataagcgca tcaagtaaca gcgaccagtt ctcttcgtta tcaaggaaac gggcgatatt
    11041 caccgcgcga aactcggtgg cgttgttgcg cagcacatcc agttccggca gctctatttc
    11101 tgcggtttca acggcaaggt cgagttcgcg caacgaagcc gccgcaagat gcgcctgaaa
    11161 atccatcagg ccgcttatcc cgaccacaca tattttcttc gcgggcaacg gccagacagg
    11221 aacttctggc gaacttagcc aggttgagcg cagagtgccg agcggggtaa ctcgctggtg
    11281 ggccagttct acgctgccct gtaattgcgc accgctttca gcgattaatg cctgcgcctg
    11341 gcaagcgaga tcgagtacgc gttgtggccc gagaagagtg taaggatggg caggtgcctg
    11401 ctgacgcaaa gcttccagtc cactgtgaat gtctgtcacc ggttgaccat ccggcagatg
    11461 gctcagcaaa tccagcgatc cagatgagaa atggagcgcg ctttgaccac gcgtgacaat
    11521 ggcacagcgc aggccgtgtt tttgcagttg caggccacag agtaatccgg cgaggccgcc
    11581 gcccataatg acagtatcaa agcgcatctt tctgctcctt ctccagacca cacagtccct
    11641 gataaaccca gcgggtaaat tcgctttcgc gcagtgcatc tccccaggcg atgggttgca
    11701 cgcctttcca gcgttcgtta aggaaggtgg aaagttgctc gatagattgc gcggacatcg
    11761 tgacgttaaa acgttgcagc agtccggcag cgcggcaggc gcagagttcg ccctggcagg
    11821 tacccatccc cacacgggta cgacggcgta aatccagcag gctattaacg tttaaatttt
    11881 ctaccgcgta ctgaacttca cccgcagtta ccgcttcgca ctcacatacc aggctacggt
    11941 gcagacggcc ttcgctcagc caggcgggtg tgcgatcgcc atgacgataa accgcagaac
    12001 cgcgcagcgg ggcaggcagg gagatgactt tacgcaaggt aacttcagcg ggctcctgtg
    12061 aaccaggaag tgccagatcg gcggttgtac aggggcgcgt gttgcccagc ttgcgacata
    12121 ccgcgtcggt agcccattcc gccatcagcc gataggtcat cagtttgccg ccggtgatgg
    12181 tgataaatcc gtccagacca tcgcgttcag catggtcgag cagcacgatg ccacgactga
    12241 cgttacggcc gctcgggtcg tcatcgctgg caaccagcgg acgcacgcca gagtaagccc
    12301 gcaaaatacg cgttttcgcc atcacggggg ccagtttttc cccttcacgc agcagaatat
    12361 caacttcttc tgccgtcact cgattatcgt caatctcatt gtaatcaata cgtaaagagg
    12421 tggtgccaat tagcgaaatg gtatcgccag gcaccagaat atcggcgtcg gaaggtttgc
    12481 ggcagcggtt gatcacatgc tgattaatgc ggtgatccat gatcagcagc gatcctttcg
    12541 ccgggaacat gcgaatgcgc agatcggcat attcggcaat gcgttgcccc cagatcccag
    12601 cggcattaac cacgacaggc gcatgaaggg cctgagtttc gccggtgaga tggttacgta
    12661 cacgaacgcc gcacaccgtc gcaccttcac gaatcagccc cgtgacttca tgagcggtaa
    12721 ggataacggc accgtgttct ttggcatcca gcatgtttgc tgcggtcaga cgaaatggat
    12781 caacggtgcc atccggaact ttcaccgcgc caatcagtgc cgggttaacg gcaggttcga
    12841 taatgcgcgc ttgctgcggg tctatagctt ctgcgctgat ccctgcttct tcgcaggcgc
    12901 ggataaaagt ggcctggaag gagaggtcat cttccggcag ggtgataaac aggccgttgg
    12961 tcggttcaac gcagtgacgt gcaatgcgtt tcaggatctg gttttcacta atacattcgc
    13021 gggccgattc cgcatcggtt accgcatagc gcgcaccgct gtgcagcagg ccgtggttac
    13081 gcccggtggc accggttgcg atgtcgtggc gctcaaccaa aatcacgcgc agtccgcgca
    13141 gggcacagtc gcgggcaatc ccggctcccg ttgcgccgcc gccaatgata atcacgtcac
    13201 ttgattgcga gtcgcgagtt ttcattgtta tccctctgaa gttcgttttt taccatttag
    13261 ccatagtaaa aacatgaatt gtttgatttc gcgcatattc gctcataatt cgaaagtgaa
    13321 acgtgatttc atgcgtcatt atgaacat
//
LOCUS       DAZGAP010000152        13287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGAP010000152 DAZGAP010000000
VERSION     DAZGAP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.153"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            426..4886
                     /gene="gltB"
                     /locus_tag="ACHLAM_003893"
     CDS             426..4886
                     /gene="gltB"
                     /locus_tag="ACHLAM_003893"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO7855350.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4899..6317
                     /gene="gltD"
                     /locus_tag="ACHLAM_003894"
     CDS             4899..6317
                     /gene="gltD"
                     /locus_tag="ACHLAM_003894"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO7855351.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6500..7627
                     /gene="yhcG"
                     /locus_tag="ACHLAM_003895"
     CDS             6500..7627
                     /gene="yhcG"
                     /locus_tag="ACHLAM_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO7855352.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(7687..8151)
                     /gene="nanQ"
                     /locus_tag="ACHLAM_003896"
     CDS             complement(7687..8151)
                     /gene="nanQ"
                     /locus_tag="ACHLAM_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO7855353.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQCDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(8148..9023)
                     /gene="nanK"
                     /locus_tag="ACHLAM_003897"
     CDS             complement(8148..9023)
                     /gene="nanK"
                     /locus_tag="ACHLAM_003897"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO7855354.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHCSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(9020..9709)
                     /gene="nanE"
                     /locus_tag="ACHLAM_003898"
     CDS             complement(9020..9709)
                     /gene="nanE"
                     /locus_tag="ACHLAM_003898"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO7855355.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9757..11247)
                     /gene="nanT"
                     /locus_tag="ACHLAM_003899"
     CDS             complement(9757..11247)
                     /gene="nanT"
                     /locus_tag="ACHLAM_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO7855356.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(11357..12250)
                     /gene="nanA"
                     /locus_tag="ACHLAM_003900"
     CDS             complement(11357..12250)
                     /gene="nanA"
                     /locus_tag="ACHLAM_003900"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO7855357.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12372..13163)
                     /gene="nanR"
                     /locus_tag="ACHLAM_003901"
     CDS             complement(12372..13163)
                     /gene="nanR"
                     /locus_tag="ACHLAM_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO7855358.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3150 a         3642 c         3585 g         2910 t
ORIGIN      
        1 ggttttctcg tcagttcaag gcaggataag ggttaacacg cctttatgac agtcaggaat
       61 tggctgtttc tctaacgacg ccccttttag ccttaaatag aaaatccact ttaatttcag
      121 caaattaata aagaattttg cgctaaagca catttctgta ccaataagct tgccatttga
      181 cctgtatcag ctttcccgat aagttggaaa tccgctggaa gctttctgga tgagcagcct
      241 gctcatcata tttatgcagt aattgagatc ccctcttcac cgtattaacc gatgcgaaaa
      301 ggacaacaag ggggcgaatg cgaggcgcgc gtatgacacg caaaccccgt cgccacgctc
      361 tttctgtgcc cgtgcgcagc ggttcggaag tggggttccc gcagagcctg ggggaggttc
      421 acgatatgtt gtacgataaa tcccttgaga gggataactg tggtttcggc ctgatcgccc
      481 acatagaagg cgaacctagc cacaaggtag tgcgtactgc aatacacgca ctggcccgca
      541 tgcagcaccg tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt
      601 tacaaaaacc ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcaa
      661 aaaactacgc cgtcgggatg ctcttcctga ataaagatcc tgaactcgcc gctgccgcac
      721 gccgcatcgt tgaagaagag ctgcaacgcg aaacgttgtc gattgtgggc tggcgtgatg
      781 tccccactaa cgaaggcgtg ctgggtgaaa tcgccctctc ctctctgcca cgcattgagc
      841 aaatttttgt gaacgccccg gcaggctggc gtccgcgcga tatggagcgc cgtctgttta
      901 tcgcccgccg ccgcattgaa aagcgtctcg aagccgacaa agacttctac gtctgtagcc
      961 tgtcgaatct ggtcaatatt tataaaggtc tgtgtatgcc ggcggatctg ccgcgcttct
     1021 acctggacct cgcggacctg cgtctggaat cggctatctg cctgtttcac cagcgctttt
     1081 ccactaacac cgtaccgcgc tggccgctgg cgcagccgtt ccgctacctc gcgcataacg
     1141 gtgaaatcaa caccatcacc ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc
     1201 agacgccgct gatccctgac ctgcacgacg ccgcgccgtt cgtcaacgaa accggctctg
     1261 actccagttc gatggataac atgctggaac tcctgctggc aggcgggatg gatatcatcc
     1321 gtgccatgcg tctgttagtg ccaccagcct ggcagaacaa cccggatatg gacccggaac
     1381 tgcgtgcctt ctttgacttt aactccatgc atatggagcc gtgggatggt ccggcaggga
     1441 tcgtgatgtc cgatggccgt ttcgccgcct gtaacctcga ccgtaacggt ctgcgtccgg
     1501 cacgctacgt catcaccaaa gataaactga tcacctgcgc ctctgaagtc ggtatctggg
     1561 attatcagcc tgacgaagtg gtcgaaaaag gccgcgtagg gccaggcgaa ctgatggtga
     1621 tcgacactcg cagtgggcgc attctacact cggcagaaac cgatgatgac ctgaaaagcc
     1681 gccatcccta taaagagtgg atggagaaaa acgtccgccg tctggtaccg tttgaagatc
     1741 tgcccgatga agaagtgggt agccgcgaac tggacgacga cacgcttgcc agctaccaga
     1801 aacaatttaa ctacagcgcg gaagagctgg attcggtgat ccgcgtactg ggtgaaaacg
     1861 gtcaggaagc ggtcggttcg atgggcgacg ataccccatt cgccgtgctc tccagccagc
     1921 cgcgcattat ttacgactac ttccgccagc agtttgctca ggtaaccaac ccgccaatcg
     1981 acccgctgcg cgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg
     2041 tcttctgtga agcagaaggc caggcgcacc gtttaagctt taaatcgccg attctgctct
     2101 attccgattt caaacagctc acgacgatga aagaggatca ctaccgcgca gatacgctgg
     2161 atatcacctt tgacgtcact aaaaccacgc tcgaagcgac ggtgaaagag ctgtgcgaca
     2221 aagccgaaaa aatggtacgt agtggcaccg tgctgctggt gctctccgac cggaatatcg
     2281 ctaaagatcg cctgccggtt ccggctccga tggcggttgg cgcgatccag acccgtcttg
     2341 tcgagcaaag cctgcgttgc gatgccaaca tcatcgtaga aaccgccagc gcccgtgatc
     2401 cgcaccactt cgccgtgttg ctgggtttcg gcgcgacggc tatttatcca taccttgcct
     2461 atgaaacgct gggccgcctg gtagacaccc acgcgattgc caaagattat cgtaccgtga
     2521 tgctcaacta tcgtaacggc atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca
     2581 tctccaccat cgcctcttac cgctgctcga aactgtttga agcggtcggt ctgcacgatg
     2641 atgtcgtggg cctgtgcttc cagggggcgg ttagccgcat tgggggggct agctttgaag
     2701 acttccagca ggatctgctg aatctgtcga aacgagcctg gctggcgcgt aagcctatca
     2761 gccagggcgg cctgctgaaa tacgtgcacg gtggcgaata ccacgcctac aatccggacg
     2821 tggtgcgcac gctgcaacag gcggtacaga gcggcgagta tagcgactat caggaatacg
     2881 cgaagctggt taatgagcgt ccggcaacca cgctacgcga tctgctggca attacgccgg
     2941 gtgaaaacgc ggtcaacatt gctgatgttg aaccggcaag cgaactgttt aaacgcttcg
     3001 ataccgccgc gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg ctggcggaag
     3061 cgatgaacag catcggcggt aactcaaact ccggcgaagg cggtgaagac ccggcgcgct
     3121 atggcactaa caaagtgtcg cgcatcaagc aggtggcgtc cggtcgcttc ggcgttactc
     3181 cggcgtacct ggtcaatgcc gacgtcattc agataaaagt cgcccagggc gcgaagccgg
     3241 gtgaaggagg tcagttaccg ggtgataaag tcactcctta cattgccaaa ctgcgctatt
     3301 cggtgccggg cgtgacgctg atctccccgc cgccgcacca cgatatctac tctattgagg
     3361 atttagcgca gctgattttc gacctcaagc aggttaaccc gaaagcgatg atctccgtga
     3421 agctggtttc cgaaccgggc gtaggcacca tcgcgactgg cgtggcaaaa gcttatgccg
     3481 atttaatcac catcgcaggc tatgacggcg gcaccggcgc aagtccgctt tcatcggtga
     3541 aatacgcagg ctgtccgtgg gagctggggc ttgttgaaac ccaacaggcg ctggttgcca
     3601 acggcttgcg tcacaagatc cgtttgcagg tcgatggcgg cctgaaaacg ggtgtcgata
     3661 tcatcaaagc agcgattctt ggcgcggaaa gttttggctt cggcactggc ccgatggtag
     3721 cgcttggctg taaatatctg cgaatttgcc acctgaacaa ctgcgccacg ggtgtagcaa
     3781 ctcaggatga caaactgcgt aagaatcact atcacggcct gccgttcaaa gtgactaatt
     3841 actttgagtt tatcgcccgt gaaacccgcg agctgatggc acagctgggg gtaacgcgtc
     3901 tggtggatct gattggtcgc accgacctgc tgaaagagct ggacggtttc accgccaaac
     3961 agcagaaact ggcactgtcg aagctgctgg agactgctga gccgcatgct ggtaaagcgc
     4021 tctactgcac cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg cagttgctgc
     4081 aacaggcaaa accgtttgtc gatgaacgcc agagcaaaac cttctggttc gatatccgca
     4141 acaccgaccg ttctgtcggc gcatcgcttt caggctatat cgcccagacg cacggcgatc
     4201 aggggctggc tgccgatcct atcaaagcgt acttcaacgg caccgcaggc cagagcttcg
     4261 gcgtgtggaa cgcgggcggc gtggaactgt acctgaccgg tgatgccaac gactatgtcg
     4321 gtaaaggcat ggcgggcggc ttaatcgcca ttcgtcctcc ggttggctca gccttccgca
     4381 gccatgaagc gagcattatc ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt
     4441 atgccgcagg ccgcgcgggt gaacgtttcg gcgtgcgtaa ctccggtgct atcaccgtgg
     4501 tagaaggcat tggcgacaac ggctgtgaat atatgacggg tggtatcgtc tgtattctgg
     4561 gtaaaaccgg cgttaacttc ggcgcaggca tgactggcgg cttcgcttac gttctcgatg
     4621 aaagcggcga tttccgcaaa cgcgttaacc cggaactggt cgaggtctta agcgttgacg
     4681 atctggcgat ccatgaagag catctgcgcg gtcttatcac cgagcatgtt cagcataccg
     4741 gttctcagcg cggtgaagag attctggcga actggtcaac cttcgccact aaatttgcgc
     4801 tggttaaacc gaagtccagt gatgtaaaag cactgctggg tcaccgtagt cgtagcgcag
     4861 cagagttgcg cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat gtttatcaat
     4921 ttatcgacct gcagcgcgtt gatccgccta agaaaccgct gaagatccgc aaaattgagt
     4981 ttgttgaaat ttacgagccg ttttccgaag gccaggccaa agcgcaggct gaccgctgcc
     5041 tgtcgtgcgg caacccatac tgcgagtgga aatgcccggt acacaactac atcccgaact
     5101 ggctgaagct cgccaacgag gggcgtattt ttgaagcggc ggaactgtcg caccagacca
     5161 acaccctgcc ggaagtatgc ggacgtgtct gcccgcaaga ccgtctgtgc gaaggttcct
     5221 gcaccctgaa cgatgagttt ggcgctgtga ccatcggcaa cattgagcgt tatatcaacg
     5281 ataaagcgtt cgaaatgggc tggcgtccgg atctgtccgg cgtgaaacag accggtaaaa
     5341 aagtggcgat tatcggcgct ggcccggcgg gtctggcgtg tgcggatgtt ctgacgcgca
     5401 acggcgtaaa agcggtcgtc ttcgaccgtc acccggaaat tggcggattg ctgaccttcg
     5461 gtattccggc tttcaagctg gaaaaagagg taatgacgcg ccgccgtgaa atcttcaccg
     5521 gcatgggtat tgaattcaaa ctcaataccg aagtgggccg cgacgtgcag ctggacgatc
     5581 tgctgagtga ttacgatgcc gtgttccttg gcgtcgggac ttatcagtca atgcgcggcg
     5641 ggctggaaaa cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt atcgccaaca
     5701 ccaaacagtt aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc atggaaggca
     5761 aacgcgtggt ggtccttggc ggtggcgaca ctgcgatgga ctgcgtgcgc acgtctgtac
     5821 gccagggggc aaagcacgtt acctgtgcct atcgtcgtga tgaagagaac atgccgggtt
     5881 cccgccgcga agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc aacgtccagc
     5941 cgctgggtat agaagtgaat ggcaacggca aagtcagcgg cgtaaaaatg gtgcgcacag
     6001 aaatgggcga accggatgcc aaaggccgtc gccgcgcgga gattgtggca ggttccgaac
     6061 atatcgttcc ggcagatgcg gtgatcatgg cgtttggttt ccgtccacac aacatggaat
     6121 ggctggcaaa acacagcgtc gagctggatt cgcaggggcg cattatcgcc ccggaagaca
     6181 acgacaacgc tttccagacc agcaacccga aaatctttgc tggcggcgat atcgtccgtg
     6241 gttccgatct ggtggtgacc gctattgccg aaggtcgtaa ggcggcagac ggtattatga
     6301 actggctgga agtttaagcc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa
     6361 attctccttt ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct
     6421 catctggatt gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg
     6481 ccatataaag ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca
     6541 aatccacgac ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt
     6601 taacgcgctg atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca
     6661 aggtggcgag gccagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt
     6721 aagtctaagg tataagcgtg ggttctctgc aaaaaactta cgccaaatga ggctttttta
     6781 cctctttttt caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg
     6841 aattccgcag acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc
     6901 caaatctttc cccctgccct ggtccaccta cgtccgttta ctttccgtta aaaaccctga
     6961 tgcccgcagc ttttatgaaa aagagacgct ccgctgtggt tggtctgtcc gccagctaga
     7021 gcggcaaatt acgacccaat tttatgagcg aacactactg tcacatgaca aatcagccat
     7081 gctgcaacaa cacgctcctg ccgagatgca tattcttccg caacaggcga tacgcgatcc
     7141 ctttgtgctc gaatttctgg agttgaaaga tgaatactca gaatctgatt ttgaggaggc
     7201 gctgatcaac cacctgatgg atttcatgct ggaacttggg gatgattttg cctttgttgg
     7261 tcggcagcga aggttacgca ttgatgacaa ctggtttcgg gtcgatctgc tgtttttcca
     7321 ccgccgttta cgctgcctgc taatcgtcga tctaaaagtg ggcaaattca gctatagcga
     7381 tgccggacag atgaatatgt atctcaacta cgccaaagag cactggacac taccggatga
     7441 aaatccgccc atcggtctgg ttctctgtgc agagaaagga gccggcgaag cgcattatgc
     7501 actggctggt ttgcctaaca ccgttctggc gagtgaatat aagatgcaac tacctgatga
     7561 aaaacgactc gcggatgaac tcgttcgaac acaggcggtg ttagaggaag gctatagacg
     7621 ccgttaattt cagcggtttc gaattctcca gacactgtct ggagaatctt taaaacgaac
     7681 taagttttaa gccattaaat cagccttaac cttcaccaca acctttttaa tctcaccagg
     7741 ttcgccgaca acgcatcctg gtttatgcgg ttctcccggc ataaacacgg cgaacattcc
     7801 cggttttaag atgatggctt gctcgttctc aatggcgctg caaagctggt aatcatcctc
     7861 atggtggaac tcttcacact gacgcgcagt gcctgccgtg ccaaacagaa tccgttcctc
     7921 accgtttaat aacagctgga tatcaatgta ttgctcgtgc agctccgctt ttttctcaac
     7981 gggcgattga gtgttaaacg tcatgacatt cataaagata ttgtcgcact gtaattcgta
     8041 acgacccggt gctttttctt gcggcctggc ggctaaggct agtgttaacg cgtcctgtaa
     8101 cgcaggatgc aatccggcag acggtaacga ctgtacttca cccattatca taatttttct
     8161 ccctgggcca acaacgcagc cccaagtaaa cctgcatcgt ggcggtagtg cgccgccagt
     8221 aaatcaacat gaaatgccgc tggctcctgc gccagatacg tttccaccaa cgccagatac
     8281 ccttctgcca gaccaacgct gccaccgacc actacgcact ggcaatcagt tgtggcttta
     8341 atatcagcga tcagccttgc cagcacatgt gcggagcagt gaatcagttg ctgcgcctgc
     8401 tcatcgccct gcccggcgcg tgtgaaaata gttctcgcat ccgcgcccgc cagttcaccc
     8461 tgcgctgccg ctgcaatacc gcgaccagaa gcaatcgctt ccacacaacc agtgcgtcca
     8521 cagccgcaga ctggaccatg aggatcagcc agcgtatgcc cgatgtgccc cgccagaccg
     8581 ccagggccgg tgagcagttt gccgccgctc actacaccgc cgccaacacc agtggaaacg
     8641 gtgataaaca ccatctcggt tatatcgcct tccagcgcct gatactccgc ccacgctgcg
     8701 gcctgcgcgt cgttaatggc aatggtcggc aaatcggtaa gttgttccaa agttttgact
     8761 aacggaaagt gtagcaatcc accgagatta tgcggattca gcgccagcag gctgccgtcg
     8821 cgaataattc cggttgaagc gatagcaacc cgctgcgcat gaacttgcaa cggagagact
     8881 aatgcggata aggcatcatg caaggcttct ggtgtctggc tggctggcgt aggaagttca
     8941 cgacgatcgc ggatctgccc atcagcgcca atcagcgcgg cggcaagttt agtaccgccg
     9001 atatcaatcg ccagtgtggt catagcaccg cctttttcat cgctgtgttg taccactgac
     9061 aaatgtgttc aagacgcgta atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca
     9121 tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg
     9181 cgtcgctcaa cgttttcacc agcgccagat ccggctcttc tggcgtttca ggcgtggtat
     9241 agccagaaag cgtagtgcca ataatttcgg ctcccagctt ttggcatgcc aggccgtctt
     9301 ccggcgttga gcagtcggtc atcgccagta aaccgtgatg gtgaatacgt gccagcagcg
     9361 tttcaacagg caccggacgc ggtcggtcgg tgccgtcaat ggcgataatg tccgcgcccg
     9421 cctgcgccag cgcatcaaca tcttcaatat aggccgtgat gcgtaccgga gaatcctcca
     9481 gatcgcgttt cacaatcccg ataattggca cgctcaccac cgcacgcgtg gcttgcagat
     9541 ttgccacacc ttcaatgcga atggcaaccg cgcccgcctg ttctgccgct aatgccatgg
     9601 cggcgacgat ttcgggttta tcgagcgggc tgtccggaac cggctggcag gagacaatca
     9661 ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag taacgacata catcttccct
     9721 tagcgaaagg cccggtacag agaccgggca acaggattaa cttttggttt tgactaaatc
     9781 gtttttggcg cggccaaatg gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg
     9841 agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat
     9901 cagcaggatc accacaaatg tcagactgaa cgagagcgat gccagcgcag tacccagatc
     9961 cagacgttga gcgatcaacg cgccgatgat tggggccagt gcaccgccca atgcgccgac
    10021 gttgtaggta aagcccaggc ccgctgcacg ctggtcggta tcgaaataac cgccaatcag
    10081 tttcggtaag atcccggcga tcccttgtcc aagcatttgc tggaagaaca gtaacagacc
    10141 gagcacccag acgtttgcgc cgccaatcgc aaataccgga ataatcagca gctgcgaggc
    10201 cagcagacta caaacgtacg ctttgcgggt tcccagccag tcaccgagga agccaccgac
    10261 gcagcacccc accgccgcgc caaagccact aaagaacagc acattggcga cagtatgcgg
    10321 gtcataagcc agatcggttt tcagatacgt tggcagcagc gcctgaatcg gccatgagta
    10381 gaggaaagca aacaacacga ccaccatcag cattacgccc gttggccagc gtttgcccgt
    10441 actctgcacc ataaagctga taaagattgc ggcgcataac agcccgagta cagcgacgat
    10501 tgcggcattt tgcaggttac cggcgaagca gaaccacagc gcagtagccg ccgccagcgt
    10561 cattacgata ttggcaatgc gatgttcacc acggtaaaga atgtctacca tcgtgcgtac
    10621 tggtgcttta cccccgtgtt tctctttcca gtcttccgct tccgggatgt ttttacgcag
    10681 ccagagagca aagatgattg gcaaaatgcc gataaagaac agcgctctcc agccccagac
    10741 cggaaccacc agactataga cctgagcggc aacgacggcc cccacagaga agcctgaaat
    10801 caaaaaacca ctggctttgt tacgcagatg ttttggccag ctttcaatga cataggtggc
    10861 gctggaaccg tattcacccg ccatccccat gccgatgacc agacgcgcga taaacatggt
    10921 gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc gagaagagaa cgatgctggt
    10981 gaccattgcc agacgacgcc cgtagcggtc acccatagcg ccgagcatca ggccgccgaa
    11041 ccagcgagag ataaaggctg cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa
    11101 ttcaccctgt acttcggtga gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag
    11161 atatcccaac caggcagcgg aaaatgcgcg ccattgtgca cggttgagat ggcgatacca
    11221 cgggatattc tgggttgtag tactcattgt gagtctcccg cggtgggcga tgcccacacg
    11281 ctttggtatg aaaattgtag ggtacagatg cgtttatttc ccctcacccg gtaggggcga
    11341 gcgaggggaa aacaactcac ccgcgctctt gcatcaactg ctgggccagc gccttcagtt
    11401 ctggcagata tttttcatct accggtccaa acggtttgcg gcacagcggc acagaaacga
    11461 catccatata atgcaggaca gttttcaggc cgcggaatac gcccgttttg atcagtaaat
    11521 caatgacttt gttgcactca gtttgcagtt tctgcgcggt ctggatatcg ccttctttca
    11581 gcgccttcac gatcccctga tatcgccagc ccatgatgtt gtaagtactg ccgataccac
    11641 catcagcgcc tgccagcaag ccggaagcga agatttcgtc gtaaccgtta tagagcacca
    11701 gatcaggatg ttcacgacgg atctgttcca tctgatagag atcgccagaa gtctgtttca
    11761 gcgcaccgac gccaggcaac gtaaccaggg tgttgatctg atccagggtc agttttaccc
    11821 cactcagggc tggaatgttg tacaccacca tcggcaaacc atccgccgaa tcaataattg
    11881 cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg
    11941 agacggcatc gaagccataa cgtttagccg atgccgcaag ttgttggctt tcggcggtgc
    12001 tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt cgcctcttca gcgacgattt
    12061 ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc ttcgccggta gaaccaccca
    12121 cgtataaacc gtcgatgccc tgctgaatat tgaactgaac caggcgacgc agactcgctt
    12181 tatccagtgc ttgttgttga tcaaaaggag tcaggagtgc ggccattacg ccacgtaaat
    12241 tcgttgccat aaatacctct gaagtgatgc ttatctgata aacgatatac ctttatacct
    12301 gttataccag atcaattaag caacacccca tacagaaagc ttataatgcg atctgcttca
    12361 ctaaagtggc attatttctt tttgttggtg gtctgaccga aggcgtgcca ggtagctgag
    12421 acgctgttga gatgcgattg caacgcacga tcggcttcat cgggatcgtg gcggcggatc
    12481 gcgtcaacga tcgcaatatg ctgttgatag ctaacgttgt tatgctcgtg cagagcctga
    12541 tcagccacgg ttggacgtgc ggcgatcagc cagtcgagca aggcaacgtg gatcgccatg
    12601 aagattgggt taccggggat ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg
    12661 aatgccgcgt tgttatccag cgactgactg ttgatttcca gtgcttttgc cagcaaatcg
    12721 atttgctcat cggtggcgtg ttcagccgca tagcgcacca ggctggattc aaagaacaga
    12781 cgtaattgtt cgaaatgggc aatcccgcca ggatgagaaa ggaaatcttt cgccatgccg
    12841 gaaagctcgc cgatgatggt gtccgcagaa ggacgtgaga cgcgagcgcg ttcgccgttg
    12901 tttatctgca ccagaccttt gcgttttaac gctgccagcg cttcgcgcac cgaaggacgc
    12961 ccgacgttaa agaacgccat cagttcgcgt tcagacggta attgttcacc ttcgccaaat
    13021 tcacgacggc ggatcatctg ttccagctct tcttccacca tttcggagag ttttttacgc
    13081 gccagcgggc ggctgcgtaa gttgcggcca attgcaggtg aagaatcttc ggtttgcgaa
    13141 tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg attaacatca tcagtgacat
    13201 cctatcacag gattgaaagt agggggaaat ggcggggttt tctctttgtg cctcatcatt
    13261 actataatta actgaataat taactat
//
LOCUS       DAZGAP010000153        13225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGAP010000153 DAZGAP010000000
VERSION     DAZGAP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.154"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..1305
                     /gene="mltA"
                     /locus_tag="ACHLAM_003902"
     CDS             208..1305
                     /gene="mltA"
                     /locus_tag="ACHLAM_003902"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO7855359.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1382..2188
                     /gene="tcdA"
                     /locus_tag="ACHLAM_003903"
     CDS             1382..2188
                     /gene="tcdA"
                     /locus_tag="ACHLAM_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO7855360.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2239..2682)
                     /gene="csdE"
                     /locus_tag="ACHLAM_003904"
     CDS             complement(2239..2682)
                     /gene="csdE"
                     /locus_tag="ACHLAM_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO7855361.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2682..3887)
                     /gene="csdA"
                     /locus_tag="ACHLAM_003905"
     CDS             complement(2682..3887)
                     /gene="csdA"
                     /locus_tag="ACHLAM_003905"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO7855362.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4079..4306
                     /gene="ygdI"
                     /locus_tag="ACHLAM_003906"
     CDS             4079..4306
                     /gene="ygdI"
                     /locus_tag="ACHLAM_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO7855363.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4657..5574
                     /gene="gcvA"
                     /locus_tag="ACHLAM_003907"
     CDS             4657..5574
                     /gene="gcvA"
                     /locus_tag="ACHLAM_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO7855364.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5593..5988
                     /gene="ygdD"
                     /locus_tag="ACHLAM_003908"
     CDS             5593..5988
                     /gene="ygdD"
                     /locus_tag="ACHLAM_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO7855365.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5981..7081
                     /gene="rlmM"
                     /locus_tag="ACHLAM_003909"
     CDS             5981..7081
                     /gene="rlmM"
                     /locus_tag="ACHLAM_003909"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO7855366.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7125..7856)
                     /gene="fucR"
                     /locus_tag="ACHLAM_003910"
     CDS             complement(7125..7856)
                     /gene="fucR"
                     /locus_tag="ACHLAM_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO7855367.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(7914..8336)
                     /gene="fucU"
                     /locus_tag="ACHLAM_003911"
     CDS             complement(7914..8336)
                     /gene="fucU"
                     /locus_tag="ACHLAM_003911"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO7855368.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8338..9756)
                     /gene="fucK"
                     /locus_tag="ACHLAM_003912"
     CDS             complement(8338..9756)
                     /gene="fucK"
                     /locus_tag="ACHLAM_003912"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO7855369.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9865..11640)
                     /gene="fucI"
                     /locus_tag="ACHLAM_003913"
     CDS             complement(9865..11640)
                     /gene="fucI"
                     /locus_tag="ACHLAM_003913"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO7855370.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11673..12989)
                     /gene="fucP"
                     /locus_tag="ACHLAM_003914"
     CDS             complement(11673..12989)
                     /gene="fucP"
                     /locus_tag="ACHLAM_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO7855371.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3194 a         3276 c         3478 g         3277 t
ORIGIN      
        1 ccgtggatgc gcactatact ctgctcgcgt tttcatgcaa cctttttttt accccgatca
       61 cggattcggc atgattttga acaaaaacaa atcactaaag cctatttttt gtgcaaaatt
      121 tgcgtcaggg agaggctgct catttcatta aacgcggtgg tttgttatct tcgttgcgcc
      181 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      241 ggcacggttg tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa
      301 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      361 gcgccgatta atgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      421 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      481 ggtgataccc gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgtcgac
      541 aactatggta acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      601 cgtcagggcg agttccagta tccgatttat cgtatgccgc caaaacgtgg tcgtctgccg
      661 tctcgtgcgg agatctacgc aggagcgctg agtgataaat atattctcgc ttacagtaac
      721 tccctgatgg ataacttcat tatggatgtg cagggtagcg ggtatatcga ctttggtgat
      781 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      841 ggtaaggtgt tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      901 cgtcactggg gcgaaacaca cagtgaagcg gaggttcgcg agctgctgga acagaacccg
      961 tctttcgtct tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg
     1021 ctggttggcc gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg
     1081 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1141 ctgatggtgg cgctggatgt cggtggcgca atcaaaggcc aacacttcga tatctatcaa
     1201 gggatcgggc cggaagccgg acaccgcgca ggttggtaca accactatgg acgtgtctgg
     1261 gtgctgaaaa ccgccccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat
     1321 gccgttttta cggatatgac agggtgaggg gtaacctcac cctttttaat tctgaggtgc
     1381 tatgtctgtg gtaattagtg atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta
     1441 tggtgaaaaa gcattgcaac tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg
     1501 tgtcggttcc tgggcggcgg aagcgctagc gcgcacgggg attggcgcaa tcacgcttat
     1561 cgatatggat gatgtgtgcg tcaccaatac caatcggcaa attcacgctc tgcgcgataa
     1621 cgtcgggctg gcaaaagcgg aagtaatggc ggagcgtatt cgccagatta acccggagtg
     1681 ccgtgtaaca gtagtggatg acttcgtgac gccggataac gtagcgcaat atatgaatgc
     1741 cggttattcg tacgtgattg atgctattga tagtgtacgg cccaaagcgg cgctgattgc
     1801 ttattgtcgg cgcaataaaa tcccgctggt cacgaccggt ggcgctggtg ggcagattga
     1861 cccgacgcag attcaggtta ccgatctggc gaaaacgatt caggacccgt tggcggcgaa
     1921 gttgcgcgag cgcctgaaaa gcgattttgg cgtagtgaaa aacagtaaag gtaagctcgg
     1981 cgtggattgc gtgttctcta ccgaagcgct ggtgtacccg cagtcagacg gcaccgtctg
     2041 tgcgatgaaa gccacggcgg aagggccaaa gcggatggat tgtgcctcag gatttggcgc
     2101 ggcgacgatg gtgaccgcca cttttggctt tgttgcggtt tctcatgcgc tgaagaagat
     2161 gatggcgaaa gcggcgcgtc aggggtaaac aatcaggtcg gatgcgacgc gaacgtctta
     2221 tccgacctgg ttctgtgctt aaacctgctt cgccgcagcg ataatcgcct cgcttaacgc
     2281 attcaacccc tggctgcgtg aggcgctaag ttgcgcacgt aatcccagct catcaaacaa
     2341 tgccagcggc gactgtgcct gcaattcggc ggcggttttc ccctcaacgg cagtcaacaa
     2401 caccgccaac aggccgcgca caatgcgccc ttcgctgtcg ccaaagaaat gcatcttgcc
     2461 gttttcagcc actgtatatc ccagccagac gcggttttcg catccggcaa tctctttagc
     2521 ctgcgctttt aactcctctg gcaaagccgg aagctgtttc cccagcatga tcaactggcg
     2581 atatttatct tcccattgcg tcaacggtgc gaaggtattg cgtaacgttt ctgcggttac
     2641 ggttgtgccg aacggatgtc cggcgaattg cgggtttgtc attaatccac caataattcc
     2701 agcgcgcggt caacggcatt caccagcgca tccacatcac tctttgtatt atatggcgca
     2761 aatgaggcgc gcagtgtgcc ggttacgcct aattctgcca gtagcggctg agcgcaatgc
     2821 tgcccggccc gcagggcaat accgtactcc gccagcagcg tcaccatatc gctgtgatga
     2881 acgccagcaa aatcaaaggc cagcaggctg gaatcctggc agcgaaatga gcggaagcca
     2941 ggacgttttg ccagcgcatc ttccgccagc gttgctaagc tacggctcca gctttcggcc
     3001 tggttgatat cgtaatctgc cagccattcc agcgccgcgc ttaatcctat gacaccagcg
     3061 acatttggcg ttccagcttc cagtttccac ggcgcagatt gagtcgtgaa gccgtcaaaa
     3121 ctcacttcgt gaaccatttt gccgccgccc agccagggcg acatcgcttc cagcagttct
     3181 gatttgccat acagcacgcc gatacctgtc gggccataca gtttgtgacc tgaaaaagca
     3241 tagaaatcaa tatccagttg ctgaacatcc gcggggaaat gcaccgcccc ctgagcacca
     3301 tcaaccatca ccaccatccc ggctgaatga gcaaaggtaa tcgctcgcgc cagatccggg
     3361 caaccgccag tgacgttcga catttgtccc agagcaagaa ttcgactacg aggtgtgatc
     3421 agttctggca acaaatcgac atccggcagt cgctgcgcat taagcggcaa tttcaccact
     3481 ttggctccag tttgttgggc gaccatcagc caggggacga ggttggcgtg gtgttctgcc
     3541 acgctgacaa taatctcatc gcccggttgc agatgcggac gcgcatagca ttgcgccacc
     3601 atgttgatgg attcagtggt gccgcgcgtc cagacgatag ttttgtcatc tggtgcattc
     3661 agtaattgcg ccactttctc ccgtgcagct tcataacgcg cggtcaggcg ttgggcttcg
     3721 gcaaactggc tgcgatggac gtttccggcg ctcaggctgt aaaactgtcg ggtggcttca
     3781 accacggctt caggtttaag cgtggtcgcg gcgctgtcga gatagacgcc cgcatcctgt
     3841 agcgcgggaa actgggcgcg aaactgcgcg ggattaaaaa cgttcatggt actcctcggc
     3901 ttgatgcacc gatcgtgacg caaaattcac tttgatacaa ggagagtgct aaccatcaga
     3961 attatctgga tatcctggcg aagccgcgtc agtaacatat gctgaatagt gttaggcgtt
     4021 aattttagcc tgttaatcaa tactattaag atttattaat cgctttaagg agaagatgat
     4081 gaaaaagact gccgcaatta tttctgcctg tatgctgact tttgccctga gcgcctgttc
     4141 cggttcgaac tatgtgatgc acaccaatga cggacgtacc atcgtctctg acggcaaacc
     4201 acagactgat aacgataccg gtatgatttc gtataaagac gctaatggca acaaacagca
     4261 gatcaaccgt accgacgtga aagagatggt cgaactggat cagtaattcg cgatcgcaag
     4321 gtaaaaaaaa gcaccgcaat taggcggtgc tacattaatc actatggaca gacagggtaa
     4381 atgtacagga agtgaaaaaa ggtagctttg ctaccatggt ctgaatcgca gaccaattgc
     4441 aaacacaaca acacaacatc acaaccgtaa gccaaaagtt caccagaaca cgcattccga
     4501 taaaactttt cgttccggct caggaagtgc cgccactata ggtatttgct ggtagaagct
     4561 caacggacaa tttataatgg ctcagattaa aaaaactaat aggttacata gtgtaatcta
     4621 attgttaaat tcatttaaca tcaaagttta aaagccatgt ctaaacgatt accaccgcta
     4681 aatgccttac gagtttttga tgccgcagca cgccatttaa gtttcactcg cgcagcagaa
     4741 gagctttttg tgacccaggc cgcagtaagt catcaaatca agtctcttga ggattttctg
     4801 gggctaaaac tgttccgccg ccgtaatcgt tcactcctgc tgaccgagga agggcaaagc
     4861 tatttcctcg atatcaaaga gatattttcg caattaaccg aagcgacgcg taaactccag
     4921 gcccgtagcg ctaagggggc gttgacggtc agtttactcc ccagtttcgc cattcattgg
     4981 ttggttccgc gactttccag ctttaattca gcttatccgg gaattgacgt tcgaatccag
     5041 gcggttgatc gtcaggaaga taagctggcg gatgatgttg atgtggcgat attttatggt
     5101 cggggcaact ggccggggct acgggtggaa aaactttacg ccgaatattt attgccggtg
     5161 tgttcgccgc tactgctgac tggcgaaaaa cccttgaaga ccccggaaga tctggctaaa
     5221 catacgttat tacatgatgc gtcgcgtcgt gactggcaga catatacccg acagttgggg
     5281 ttaaatcata tcaacgttca gcaagggcca atttttagcc atagcgccat ggtgctgcaa
     5341 gcggctatcc acgggcaggg agtggcgctg gcaaataacg tgatggcgca atctgaaatt
     5401 gaggccggac gtcttgtttg cccgtttaat gatgttctgg tcagtaaaaa cgctttttat
     5461 ctggtttgtc atgacagcca ggcagaactg ggtaaaatag ccgcctttcg ccaatggatc
     5521 ctggcgaaag ccgctgctga acaagaaaaa ttccgctttc gttatgaaca ataatttacg
     5581 tagggtacga ccatgaccag ccgttttatg ctgattttcg ccgccattag cggcttcatt
     5641 tttgtagctc tgggcgcttt tggcgcgcat gtgttaagta aaaccatggg ggccgttgag
     5701 atgggctgga tccagaccgg cctcgaatac caggcgtttc atacgctggc gatcttaggt
     5761 ctggcggtgg caatgcagcg tcgcatcagt atctggtttt actggagtag cgttttcctc
     5821 gcgttaggca cggtgctgtt cagcggcagc ctttattgcc tggcgctgtc ccatctgcgt
     5881 ttgtgggcgt ttgtcacacc ggttggcggc gtgagcttcc ttgcgggctg ggcgttaatg
     5941 ttagttggtg ctatccgttt aaagcgcaag ggcgtaagtc atgaataagg ttgtattgct
     6001 gtgccgtccg ggctttgaaa aagagtgcgc cgcagaaatt accgataaag ccggccagcg
     6061 ggaaattttc ggttttgccc gcgtgaaaga gaatgcgggt tatgtcattt atgaatgtta
     6121 tcaacctgat gatggcgata agttaatccg tgagctgccg ttcagttcat taatttttgc
     6181 ccgccagtgg tttgtggtgg gggaactcct gcagcatttg ccgccagaag atcgtattac
     6241 ccccattgtc ggcatgttac agggtgtagt agagaagggc ggtgaactgc gtgttgaagt
     6301 tgccgatacc aacgaaagca aagagttgct gaaattctgc cgtaaattca ccgtgccgct
     6361 acgcgctgcc ttgcgcgatg cgggtgtgct ggcgaactat gaaacgccga agcgtccggt
     6421 tgtgcatgta ttctttattg caccaggctg ctgctatacc ggttactcat acagcaacaa
     6481 taattcgccg ttctatatgg gcattccgcg cctgaaattt ccggcagatg cgccgagtcg
     6541 ttccacgctc aaactggaag aggcatttca tgtgtttatt cctgcggatg agtgggatga
     6601 acgcctggcg aacgggatgt gggcggtgga tttaggcgct tgcccaggcg gctggaccta
     6661 ccaactggtg aagcgcaaca tgtgggttta ttccgtcgac aacggcccga tggcgcaaag
     6721 tctgatggat accggacagg tgacgtggct gcgggaagac ggtttcaaat tccgtccgac
     6781 acgcagcaat atctcctgga tggtatgcga tatggttgaa aaaccggcga aagttgcggc
     6841 attgatggcg cagtggctgg ttaatggctg gtgccgtgaa accattttca acctcaaact
     6901 gccgatgaaa aaacgctacg aagaagtgtc acacaatctg gcgtatattc aggcacagct
     6961 tgatgaacat ggcataaatg ctcagattca ggcgcggcag ttgtatcacg atcgcgaaga
     7021 agtgacggtg cacgtccgcc gtatctgggc tgcggtgggt ggtcgtcgcg acgagcgata
     7081 acaggcaagg ccggggcgtc gtttccggcc tgctatcctt tccgtcaggc tgttaccaaa
     7141 gaagctgcaa cctggcgctc atcagaaata atgtgcgtga cctcatccag atgcccgatg
     7201 cgggcttccc ctgaacgatt aaatttactt ttatcaatca ataacaacga ttgcgcggca
     7261 cgttttaata gcatcgattt gtaatcagcg ttgatggcat tggagtccca cagtgcgccg
     7321 ctgctatcga tcccttcaca agaaaaaata aacagatcga tttccagcga tttaagctgg
     7381 gaaatcagcg agggattgac gtaacaacca tatttgcgct caagtgtgcc gccggaactg
     7441 atcagttgaa tgcgttcgcg tttaccgagt tcatggcaaa tcggatggct attggtgaag
     7501 acctggatgt tgatgtcagg caactggcgt gccagatacc agcaggttga acttgcgtct
     7561 aaggctatta ccatcccttc ttcaatccac gcgagcgcct cgcgcgcgat atctgcttta
     7621 tgcgcataat ggcttttcag ccggatgtga aagggatcgc cactgtcttg attttgacgg
     7681 tggatatatt tagcgcgccc atgattgcgc agaattttac cctgcgtctg taattcattg
     7741 agatcgcgac gaatggtttc tttactgacc tttagctgtt cagagagagc ttccgtggtc
     7801 aggctggtat ggttcagcag caggtcgact atcgcttgct ggcgtgccgc tttcatattt
     7861 ttcaccccat cattcgataa ccgcccgcgt catgcgggcg taccggtatg agattacggt
     7921 gttacccctt tttttaaaag aatattcccg tacttcgctc gttcgcctgt gataacgatc
     7981 gcaaaggctt tttgtgcccg ttcataaaac gcaaaacgat tgatgcggat gatgtcagga
     8041 cagggggctt gtagtgaaag cgcattacga taacgtcgtt ctacttcagg atctagagtg
     8101 tcaccttcta ccgccgccat catcaccagc ggtggtgcat aactgtccag ctcaaataac
     8161 gggataatcg cctggagcaa gtcgctcacc aacaggccat cagcgcggat cacctgcggt
     8221 cccatcgaat gggcgggaaa gtgagcatcg gaaaaaataa tttcatctcc atgccccatc
     8281 tctgccagca ctttcaatag ttcgggagaa attaacggcg aaattgtttt cagcatttca
     8341 cacctcctct ataaattcag gttcagtttg cgggtagaaa taacggtact gataatgaat
     8401 ctgcgcgcgg gcttcttccg gactgttaaa ttcccctacg ccataccaac caaacagcgc
     8461 agctcctgcg acggtcgttt cggcatcgtc gagaactttt accggaatat caagcatatt
     8521 ggctttaatc tgattccaca atgtgttgcg acttcctccg ccgactaaca ataattcaga
     8581 ggctttaaag tgcccgattt tttccagcat ctgtagattg cgctgtaatt gcgtggttaa
     8641 cccttccagc gccgcgcgat agaaatgccc ccgcgtggta ttaagcgtta cgccttgcca
     8701 gccagcgttc tggcacgaca ataaatcaca ctgcattttc actccatctg cgccaggcgc
     8761 gatcaggcga gcttcttcaa tcaatatttg ccagggagtt tcagctgtcc agaacagttt
     8821 tctcacccat tccagcacgc cggatgccag ccattgcata cctgggttat acaaccctgc
     8881 ctggctatcc agttcgcaag tagaaccggc gtactgactt aacagcgaag tatcaacctg
     8941 ggcgctgcgg accattaaaa tttcccatgt accggaagag agcacgggtt cattttgttc
     9001 ggcaccagcg ccaaaaaggg cgaactgggt atcgtgacct gcggaaatca ccggtatgcc
     9061 aacgggtaag ccgagcattg ctgcggcgct gttctgtagc gtaccaatct gttcacccgc
     9121 ttccaccaga cgagggaaga gtcggcgtgg aataccggtg gcttgtaaaa tttgcggact
     9181 gaaatcgcgt tgctggatat ccagcatctg gctggttccg gccatcgtaa tatcagtagt
     9241 gaattcgccg gttaaacggt ggttaatcag tgacgaaata aagagccagg cgtgcgcgcg
     9301 ttccagcagt tgtggatgat tttctttcaa ccacaccaac ttatataacg tattgaaact
     9361 aaaggctccg actccagaaa tagcctgcaa ctgctgtgcg gagatcaacc gttcaatatt
     9421 gtccattacc gcggctgttc gcggacattt ccagctaatg atcggataga gcagattgcc
     9481 ttgcttatct accagagcgc catccacacc aaaggtggtg acggcgatac cgcggatatg
     9541 gcaatcagtc agttcactat tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc
     9601 gtctaaagac cactggtgcc aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt
     9661 tgaggcgcgg gcaacaattt ttccctgccg attaaccgcg atggccctga cattggtcgc
     9721 gccacagtcg agtaccagga taacttcttg tttcataatc gctcctgcaa tatagccgga
     9781 taacattgct tatccggcta accactcttg cttgttcagg cccggtagcc ggagcgaccg
     9841 ggcatcacat cagggagtca tgtattaacg cttgtacaac gggccgtagt tctggcaagc
     9901 gcggtaatcc tggccttcaa tatccatgcc gtgcgcggcc caggcagaag gacgatacac
     9961 tttggtctct tcaacgttgt gcatacatac cgggatacgc agcatggagg caagagtgat
    10021 aaagtctgcg ccaacgtggc cgatggtcag aactccgtgg ttagcgcccc agttcgccat
    10081 taccgagtac acatccgtaa acggcccttt accggtgagg cgcggtgcaa accaggtggt
    10141 tggccaggtt gagttggtgc gtttgttgag gatgtcatgc acatccttcg gcaattccac
    10201 gctccagcct tccgcgattt gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt
    10261 catggtgaac gggacgccgc cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa
    10321 gtattcgtgg atcgccgggc accattcggt agcggcgagg caagcgtcag cctcttgctg
    10381 agagatttcc cagtgcggct tcatcgtcgg tttaccttcg ctgtcgcgtt gtttacagga
    10441 gccgtccagc gcagcagaac cggagttgat caaatggatg atgccgtgtt ctgccagccc
    10501 gtccagttta tgccccgtta cacgctcaat tgcttctggt gaccagtagg tacgcacatc
    10561 ggcaaatatc tgagcggtgc cggtgagctg gtggcccatt aacattgcca cgccgttaag
    10621 actgtcgttt tcggtcgcca cgacaaacgg tttgcgcacg ccattccagt caaatgaact
    10681 gttgaggatc gcttcagcgg tatcgccatt gggatattga tcggtccagt gacgttgccc
    10741 ctggaagcct gcagcgatgg cgttgtagcc aagtgattct tccacgcggc caatatcggc
    10801 cagtttgctg ttgccttgca tcatgtcgcg gatacacatc gccatcagta aactttcgcg
    10861 cagaactgcg cggctttgct cggcattgcg ttgatactgt ttgttatttt catcttcgcc
    10921 atagcggaag tttttatcag cccaggccag cgccatttcc agttcggctt cgtcgtaaat
    10981 cttctgatcg atacggcgac gcagttcggt catatccacc gcctggactt tcattcccag
    11041 ccaggattca aagaagttgt gatcaacgat ggaaccggcg atacccatcg aaacgccgcc
    11101 cagcgacaga tagcttttac ctttcatgct ggcgactgct aaaccggcgc gggcaaagcg
    11161 cagcagtttt tcttcaacat cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg
    11221 accgtaaatg gagaatgccg ggatgccttt ctggctgtga gctgccagag ccgctgccag
    11281 gtaaacagcg ccgggacgtt cagtgccgtt aaagccccaa atggctttcg ggcgggttgg
    11341 atccatatcg atggtttcac tgccatagca ccagcaaggg gttaccgtaa tggtgaggcc
    11401 tacattctga ctgctgaatt tttcttcgca agcagcggct tcagccatac ccgcgataca
    11461 ggtatcggaa atgacacact cgacggcagc tccacaggca tggcgcagtt tctcggtcag
    11521 cagtgcagcc gtggctttcg ccatattcat tgtttgttct tcaagcgact cacgaacacc
    11581 catgcgacga ccgtcaataa ccgggcggat accaattttc ggtaagctga tttttttcat
    11641 tacagattcc tcactttatt cggaaaatat gttcagttag ttgctgtttg agaacggaaa
    11701 cgggcaaaga taaagatgac cgcgaagcag agtgcgggga tcagttcagc agtggggatg
    11761 ttgcccgccg cgtcactgac aaaacccatg accggagtga caataccgcc gccaatgatg
    11821 gtcataacga tgaaggacga accgtatttg gtgtcctggc cgagattctt aatgcccagc
    11881 gagaagattg ttgggtactg aatcgacata aaggcgctgc ataaagtcag ggctattaag
    11941 cccacatgac cgccagcgaa ggctgagatc aggcacagtg ccatagcgat taatgcgtag
    12001 gctgccagga ctttgtgtgg tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca
    12061 ataaagaagc acaccatggt tccggttaaa tagttagcgg caaagcctgc ggtcatacct
    12121 ggaatttctt ctacagcgta gcgaatcaaa tagctccagc aggccgtttg cgcgccgaca
    12181 tagcagaatt gcgctaatac cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc
    12241 gatgcggaga acgatccttg tttggcgtca ctgtgattat cactctgcaa tgccgggaat
    12301 ttcgtcagca tgatcagcag ggcgaccagt aacacgatag ccacgatgat catataaggt
    12361 gtctgtaccg ataataccag gctgtgttta tacgcactca attgctctgg agacatttta
    12421 tcgagaacgt cttgcgattg atgtggcacg ttagacaaaa taagactttg tccaaagaca
    12481 accgcgataa ttgcgccaaa cgagttaaat gtttgcgcaa gatttaagcg gaagtgacca
    12541 ctgctttccg gccctaatac cgtaacaaaa gggtttgcgg cagtttccag acaacctaat
    12601 ccagctgcaa taataaatag gccaactaaa aataaggtgt agttcattat ttctgcagcg
    12661 ggccagaata atgcagcgcc caaggcatat aaaaataatc cggtaataat ccctgcttta
    12721 taactgagtt ttttcatcaa tatcccagca gggattggga taatgaaata accaaagtaa
    12781 aaggccgatt ggatcaggcc agcctggaaa tttgtcagcg taaaagcctg ctggaattga
    12841 ggtaataaaa tgtcgttaag gttattggct accgcccaaa gaaaaaacag tgagcacagc
    12901 agcgcgaatg gaataatgta acttctgctt tgccctgcat ctttatctac cgcacggtaa
    12961 ctctgcgttt gtattgatgt gtttcccata gcatcctctt aggttcagaa gcttaagcac
    13021 gtaatcggtt atgttttcaa aagtgcaaaa ccagcgagtt cgggcttcat atcagtgaat
    13081 atttgcctgt cagaaatgaa ctcatggatt cattttcaaa taaaaaacgc tctgtaatat
    13141 gacggcggtc acacttatta gttgggaggt gactttatgt gactaccatc actttaataa
    13201 tcgaaatttc atgcccgttt cagaa
//
LOCUS       DAZGAP010000154        13223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGAP010000154 DAZGAP010000000
VERSION     DAZGAP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.155"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            154..462
                     /locus_tag="ACHLAM_003915"
     CDS             154..462
                     /locus_tag="ACHLAM_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO7855372.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(521..1615)
                     /gene="yaiW"
                     /locus_tag="ACHLAM_003916"
     CDS             complement(521..1615)
                     /gene="yaiW"
                     /locus_tag="ACHLAM_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO7855373.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(1628..2848)
                     /gene="sbmA"
                     /locus_tag="ACHLAM_003917"
                     /pseudo
     CDS             complement(1628..2848)
                     /gene="sbmA"
                     /locus_tag="ACHLAM_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            3200..4357
                     /gene="ampH"
                     /locus_tag="ACHLAM_003918"
     CDS             3200..4357
                     /gene="ampH"
                     /locus_tag="ACHLAM_003918"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO7855374.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRVQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(4358..4981)
                     /gene="iprA"
                     /locus_tag="ACHLAM_003919"
     CDS             complement(4358..4981)
                     /gene="iprA"
                     /locus_tag="ACHLAM_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO7855375.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(5067..8015)
                     /gene="ehaB"
                     /locus_tag="ACHLAM_003920"
     CDS             complement(5067..8015)
                     /gene="ehaB"
                     /locus_tag="ACHLAM_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO7855376.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWADEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
     gene            8541..9515
                     /gene="hemB"
                     /locus_tag="ACHLAM_003921"
     CDS             8541..9515
                     /gene="hemB"
                     /locus_tag="ACHLAM_003921"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO7855377.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(9555..10406)
                     /gene="tauD"
                     /locus_tag="ACHLAM_003922"
     CDS             complement(9555..10406)
                     /gene="tauD"
                     /locus_tag="ACHLAM_003922"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO7855378.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWCWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(10403..11230)
                     /gene="tauC"
                     /locus_tag="ACHLAM_003923"
     CDS             complement(10403..11230)
                     /gene="tauC"
                     /locus_tag="ACHLAM_003923"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO7855379.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTFSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(11227..11994)
                     /gene="tauB"
                     /locus_tag="ACHLAM_003924"
     CDS             complement(11227..11994)
                     /gene="tauB"
                     /locus_tag="ACHLAM_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO7855380.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVVFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(12007..12969)
                     /gene="tauA"
                     /locus_tag="ACHLAM_003925"
     CDS             complement(12007..12969)
                     /gene="tauA"
                     /locus_tag="ACHLAM_003925"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7855381.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         3319 a         3474 c         3183 g         3247 t
ORIGIN      
        1 ttttgtgact cacgacgaag attcacgctt cgggaatatt cccaacatca atttaccatg
       61 attctgatat tgacaatctt aatgatctgt cagacttgcc ataagccgct ggtatcacac
      121 cagaagagaa gcagatgact tccggaggtt tttatggctg atttcaccct gtcaaaatcg
      181 ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg gcaacattgc ctatgcgttg
      241 tttgtgctgt tttgcttttg ggctggggcg caattgctga acctgttagt gcatgcgccc
      301 ggcgtctatg agcgcttaat gcaggtccag gaaacaggtc gcccacgggt ggaaattggt
      361 ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag ttggctgcct catttttgca
      421 gtagtggcgc tatggctgca ctggcgacat cgccgccagt aagatttacg ttgccagatg
      481 cggcctaaaa cgccttatct ggcatgacat tcctgtttga tcactgtttc atacaacgcg
      541 cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg
      601 ggctttccag ttgaatgcca ggcaacattt cgcggggtag tgatttaccc gttttcgctt
      661 cggcaagttg ataaactttc ttgtacagcg ccgtctcctc aaaagagaag ctgtcccctt
      721 tctctaactg gcggcgaatt tcactgtcgc tcatccccag ttttcccgcc agtttgcgcg
      781 ttgccagttc ggttttcccg ggctctttac tgtcgtaacg aattaaatca ccgtccagtg
      841 ccagcttcac gccgctggcc ttactgaccg cgttctgaaa cgcggcatta cggctggcgt
      901 accagccagc gttaaaatca gcaaaacggt atatcggcgc gctatagctg gcgggataat
      961 tcagtaaatg gtaagtacca aaccacaacc cgccgcgacg actgaagact tcctgacgga
     1021 ctgtaccgtc cattttccac ggataccctt tggtatgctg ttcggcaaag gcaatgctga
     1081 cctgcatcgg cccaccggtg cgcaccggat taagcgaacc aaacaacgtc tgccccattg
     1141 gcaccatgct gatcagatcg tcaaaaatcg cgctcaattg cttttcagta cgcaccgaat
     1201 ctaaccgttc gctatagctt ttgccgtttg gcgatttgat ttttagtgcg gtatgcacca
     1261 gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat ctcttgccag gcgatcttgc
     1321 ttaagcccgg aacagccgga tctgcctgat aatttgactc ctgctgcgcc accgccagca
     1381 ccgagcagac gttttccagc gtcggtgcca gcccctgact ttcaaacgtg gtggcgagat
     1441 ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat tttctgccgc acgacgctcg
     1501 ccacatctat cgctttttcg ccctttttca acggttgcgg agcctgacta ctacatcccg
     1561 ctaaaaccag aaccgccaac aatgacagcg aagatagatg attgacgcgc gacattactt
     1621 ctccttttta gctcaaggta tgggttactt cctgaatttt gtcaccatcc agctcatgtt
     1681 caaagctgcg cagacgtttg tagatagaca tcaactcaac cagtgtggtc catgagttaa
     1741 tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt agtaatctgc gtcatcaggc
     1801 cgagtgtaat cgtaccggca acaattgacg gaaacagcaa gaacaaaccg aaaacgttat
     1861 cgacctgcaa atagagaatg cgggcgatgt tgaaatacat atagtgaaaa tagaggcgga
     1921 aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt aggcggcgtc gcacgcgtgg
     1981 catcgtcttc accataaacc agctctttac ggtaggcagc ctctactcgc tggtttttaa
     2041 actccagccc cggcagtttg atccctacca ctgccagtaa tccggtcccc atcagcgacc
     2101 agacgatagc ggcaatcacc agaccatacg gaatgtgccc gacaatcggc agctccggca
     2161 catgtgcgga gagcgttacc agcaccggca ggaaggcgat caacgtcatg atagcgttga
     2221 taaagctgac gcccatattc tccagcgttg aagcaaaacg catggtgtct tcctgcacac
     2281 gctgcgcggc cccttcgata tgacgcagtt gttgccagtt cgccatgtaa tattcgttca
     2341 tcgccgtacg ccagcggaac acgtagtgac tgacaaagaa gttgttcagc acactgatca
     2401 ccaccgcgat cagcgcaatc cccagaaaga ctcccacttc gcggtaaaat tgttcgatgg
     2461 tgactttatg cggcgaactt agcgccgttt gaatcagatc atagaacggc gcataccagg
     2521 cgttgacggc aaccccgact tccaccaaaa accaggtgac gaagataatc agcgcagtac
     2581 cgagtattga ccagtattgc caacggtgcg ggctatagat aaaccagaac aatgcaaaaa
     2641 gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg
     2701 cggcgctaat cgggatctgc ccggaagcgc cggtgatacg cgccacccaa tcccccccac
     2761 cggcttacca gaagataacg gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc
     2821 ccggctttgg gaaaaaagac ttaaacatat gcactcctgc taacttctta tcgttttaac
     2881 aactgctgtg tattaaccgc atgaccgcta caaagcgtca gccaaatgaa accgcccatt
     2941 gtgacccata gcaaggcgct tagttcgcca gcctgagcat aagaattgtt tcgacggatt
     3001 tcacaaacca aagattgttt acataagcgg attaacacgc gcaggatcac aaatgcaggc
     3061 attggtgagc aggaaaaaga tcgtgtttac gcgccgttac gtttttaaca ctattattgc
     3121 actaattcgc cctttgcaat ccatcaatga gtagtataaa tacgctcagt taccttcatt
     3181 caatctatgg acaccaccgt tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc
     3241 gtcattgacc tccgtccatg cggcgcagcc gatcactgaa ccggagttcg cctctgatat
     3301 tgtcgatcgt tatgccgatc atatttttta cggcagcggt gccacgggga tggcgctggt
     3361 tgttatcgac ggtaaccagc gcgtctttcg cagttatggc gaaacgcgac ctggtaataa
     3421 cgttcgcccg cagctggatt cggtcatacg tattgcttcc ctcaccaagc taatgaccag
     3481 tgagatgctg gtgaaattgc tcgaccaggg gaccgtgaag ttaaacgatc cgctaagtaa
     3541 atatgctccg ccaggcgcac gcgtaccgac ttacaatggg acgcccatta cgctggtgaa
     3601 tctggcaacc cataccagcg ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc
     3661 ggtatttgtc tggccaacgc gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa
     3721 agccgcgcca ggcagtcagg cggcgtactc taacctcgca ttcgacctgc tggcagatgc
     3781 actggcgaat gcgtccggca agccttatac ccagttgttt gaagagcaga ttacccgtcc
     3841 attggggatg aaagacacca cctacacccc ctcaccggat cagtgccgcc gtttaatggt
     3901 tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg gcaattggca gcggcggcgt
     3961 ctattccacg ccaggcgata tgatgcgctg gatgcagcag tatttgtcat ctgattttta
     4021 tcagcgcagt aaccaggctg atcgcatgca gacgttgatt tatcagcgcg tgcagtttac
     4081 aaaagtgatt ggcatggatg tccccggcaa agcggatgcg cttggtttag gctgggtata
     4141 tatggccccg aaagagggtc gtccagggat tattcagaag acaggtggtg gtggcggttt
     4201 cattacctat atggcgatga tcccgcagaa gaacattggc gcatttgtgg tggtcactcg
     4261 ttcgccattg acgcgcttca aaaatatgag cgatggcatc aatgacctgg tgaccgagct
     4321 aagcgggaat aaaccgttgg ttatccccgc atcctgatta atactctgaa ggcaaacggt
     4381 tgatagcgac cagtttgcct ttattcattt cgatatagcc gcctttacgc aaagcagcga
     4441 gaacttccgc aacgacagaa cgcgatatac gtgtacgttg atggatataa ttcatcacac
     4501 caatacgtga tcgcaattct tcattccagt caatcattga taataacgtc gcgcggattt
     4561 gttcgtagga attatgccca atgagctgaa cgtcgcgtaa ttccagaatt ctattttgcc
     4621 aggctaacca gtaaaaagcg tctcgccaga gttgattttg ttcaataaga gtaatggttt
     4681 gtttggctgg taaatgatat cccgcacagc ttccttctga tattaatttg tatggtatat
     4741 cgtttttcat taaaccatca gccagcccca taatataagg agcctgggta ataccgataa
     4801 gtacgttttc ttctctacgc agagagataa cgccctctaa aataacgaaa gtatcttcgc
     4861 tattgacatc actggaaaat ataacttgct tttcattatt aaactcgaag cgcgtaccgt
     4921 atctggacaa acatttatcg agcttaccaa attcctgaag aggtttaact acagataaca
     4981 tttgtgcgtc ctttgcagta atgcccgtca aatccttgac gggcattatt tagattaaat
     5041 tacctatttc ttcggagtga agaatattac caggtatatt taacacccac attcgcggac
     5101 cagtcttgat ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc
     5161 ttggtgaagc tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta
     5221 tcgatggaat caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt
     5281 ttgaagtacg gagtcagagc ctggtcttcg ctgtaggtga aggtataacc tgcatctaca
     5341 cccagttcat aacgcatgct gtcgtaagac tgaccatcaa ctttcatgtc gttgctcagc
     5401 tggtaatcat caccagactg gaacagacca gaaacgctgc cgtaaggagt cacgtaacct
     5461 gcatcaccca gtttgaagtc gtaaccggct ttcaaaccaa agccccaagc gtcggagttg
     5521 gtgctgccgt caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga
     5581 gagtagctca agctaccgtc aacaaagacg ttgttcgcga agtgagcaga agagtagatg
     5641 taggcagtct ggctgtcttg atccacctga ccagaacggt cattcatatc acctttagcg
     5701 aagcctgcag ccgcaccgac gatccactta gcgttgttac cgtcaatttt ggtatcaaca
     5761 ccgaccatga tgccgttaac atcctgatca tagttgatgg tgccgttgtc gccgttgaag
     5821 ttaccaccga agtagcttac ccatgcgccg ccgttatcag ccagaccatg acgagagttg
     5881 gtcagacgag taccaacggt gtcttgttcc aggttccaga tattggtgtt cgcagacggg
     5941 atgctcagcg ccatgttagc gtagtcggtc agctccatct gttgcagaac aacggtgtta
     6001 ccacgctgct cagcctgata ggtgtatgca cccaggtcag ctttgttagc agcagagaag
     6061 gtcgcgttgc tgtttttgtc gttgacgtag atcagctcgt tgccgttgta gtcagcgata
     6121 gaaccagcac cagtagcgtt gtcgatacga actttgtagt taccggttgc agctgcaaca
     6181 tggtcaccgt tgttatcaac ttcgttctga ccagcttcga tagaagccgt gttgtcgtta
     6241 tcgccgttac cgttaatggt caggtgacca tcagagttca ttgcgattaa gccataaccg
     6301 tagtttgact tggtagtatc gttggtacgg tcgttggtca gatcggcatc gagtacgaag
     6361 cggttgcttt cgatgttgaa cacacctgcg tgaatgttac cactggtatc agtagtgctg
     6421 gtcaggttca gagtatcaac ctgcagagca ttcgccgcag tgttacctac atccaggata
     6481 ccgtcgttag tcactttgat gttgttagca tacagagcag catcacctac agcccagcca
     6541 gcagaaactt catcagaaat ggttacggta ctgttgtcga cagtcagtga atcagttgca
     6601 acatgaccat cttcgttgat gttcagcgca gaaagaccag tcagagagat agagtcagaa
     6661 atcagcgttg aatcagcaac attaacagtt gaaccactgt tgatgctcag agtatcaatc
     6721 agagaagttt tggtggtatc ccactgtgaa ccggtgttca gggtcacatt gaacagaccg
     6781 ctctggtaaa cttcgttacc gatgatgtga tcaaattcgc tgtattcaga gtcgctgttt
     6841 tctacaccaa cagttgatga aggccacagg ctgttagctg cgatgtcgat cagagttgca
     6901 gtggcttcag tattgattcc atatgtatag gagtcattta caccatcgtc atcagtatca
     6961 acaccaatct gatgcgcaga cattgccgca ccgacccact tgctgccatt gttcagggtc
     7021 agatccaggc gatcagtacc atcccaaccg ttagtatcta catcaccgtc agcatcacga
     7081 tagctatcac gaccttgtgg gaagaagttc tcatcaaaat tacttgagaa gaatacatcg
     7141 cccatcagtg tggaattgtc gaatgttgca gtggtttgca tggcgttatc agcatatgga
     7201 tgtgcaataa cagctaatgc aacatcatca gcattccacc catcaccatt gtaatcacta
     7261 gcattaccag tattaccaaa ccaaccagta gtaccttcat cagcccatga accagaggtc
     7321 acggtagaat ctttaacagt gatggtgtta ttaaatactt cgctgctgtt tacaccagtg
     7381 ctgacagtgt tgtcgtcgat atcttcccac tcataaccct gagtcagcgt aatacctgct
     7441 acatgagagt tattagagat ggacagatcg acttcctgat ccagagtgat ggcagtacca
     7501 atgttaaata catttacaac atgagtgtca gcggcattat tgtaagtgcc gttatatgtg
     7561 tagtgttcgt agttgtcatc gatcgttgaa ttatcaacgg tcagcgccag acgatcatgg
     7621 tagtagtcat ccgcacgatc agcacaatca gttgtcatgc actcggaata aatcataccg
     7681 tgaatagtgc tgtttttgat tgtcaggcta ttggcgttgg tattagtaga aataccatcg
     7741 tccagatagt aggtagaaat cacgccgtta acggtagcgt tattaatgac cggatagata
     7801 tcgccgttgt aataactatc agcggcatag ttattccagc ctacataacc atcatagtaa
     7861 acgtcatcag cataggtggc gtcgttgtag tgatggaaag tattgtaagt ttgaccagaa
     7921 atatcgttgg tcgctgcatt agcctgagag gtgatagcca gagtgcaagc caatgctaat
     7981 tgtgatacta caagtttctt tttccaggag tgcatttgtc atccctcctc agggacgtaa
     8041 taagttgatc catcaattat caatgcatag aaaacatatt atgtttcgcg tttacgatta
     8101 tgcggcgtta tagaggaatg gttcaattat tctttcccct aagtccgatt gcggacatat
     8161 ataaagcacg tgaacacaca caaagaaaga aataacaaaa acacaatatt tcaatacgtt
     8221 aacgaaagcg ttaattttaa aacatagacc atgaaaatat aaaacaacca aaacagacaa
     8281 ataatgtatc aataagcaat gaactttaag ttattcactt tttgaaaacg tcaccaaatc
     8341 cccaaaaaca ataatctatg gctataaata aaaattaaaa atattttcag tgaatatgaa
     8401 attattgcta tttctattac aaagatgtcc tgacctcttt ttcattctct tcttgtcaac
     8461 atcgcgacaa ctttcgtaaa acacacccag cctgcttcag gtatactatg ctcctcgatt
     8521 ccacaaacat caggcagacc atgacagatt taatccaacg ccctcgtcgc ctgcgcaaat
     8581 ctcctgcgct gcgcgctatg tttgaagaga caacacttag ccttaacgac ctggtgttgc
     8641 cgatctttgt tgaagaagaa attgacgact acaaagccgt tgaagccatg ccaggcgtga
     8701 tgcgcattcc agagaaacat ctggcacgcg aaattgaacg catcgccaac gccggtattc
     8761 gttccgtgat gactttcggc atctctcacc ataccgatga aaccggcagc gacgcctggc
     8821 gggaagatgg actggtggcg cgtatgtcgc gcatctgcaa gcagactgtg ccagaaatga
     8881 tcgtcatgtc agacacttgt ttctgtgaat acacttctca cggtcactgc ggtgtgctgt
     8941 gcgagcatgg cgtcgacaac gacgcgactc tggaaaattt aggcaagcaa gccgtggttg
     9001 cagctgctgc aggcgcagat ttcatcgccc cttccgctgc gatggatggt caggtacagg
     9061 cgattcgcca ggcgctggac gctgcgggct ttaaagatac ggcgattatg tcgtattcga
     9121 ccaagttcgc ctcctccttt tatggcccgt tccgtgaagc tgccggaagc gcattaaaag
     9181 gcgaccgcaa aagctatcag atgaacccaa tgaaccgtcg tgaggcgatt cgtgagtcac
     9241 tgctggatga agcccagggc gcagactgcc tgatggttaa acctgccgga gcatacctcg
     9301 acatcgtgcg tgagctgcgt gaacgtactg aattgccgat tggcgcgtat caggtgagcg
     9361 gtgagtacgc gatgattaag ttcgccgcgc tggcgggtgc tatagatgaa gagaaagtcg
     9421 tgctcgaaag cttaggttcg attaagcgtg cgggtgcgga tctgattttc agctactttg
     9481 cgctggattt ggctgagaag aagattctgc gttaatgatc attatgccgg gttatccaac
     9541 ccggcatttc ccccttaccc cgcccgataa aacggtttat ccccaagaat cgtcgcccga
     9601 tgcattatcc gccgctgtgg caggtaatcc gcattggcat agtgctgggt tacgcggtta
     9661 tcccaaatcg caatatcatt tggttgccag caccagcgca cctgaaactc cggtttggtg
     9721 atgtgggcaa ataaaaagcc cagcaaggct tcgctctctt tctcgctgac atcaacaatt
     9781 cgcgtggtaa agccttcatt cacaaacagc gcctgtttac cggtcaccgg atgcgttcgc
     9841 accaccggat gtagcaacgg tggatttttc gcgaccgcct cacgccagcg ttgatgctca
     9901 gcctcggttt tgcggtactt gtattccggg aacgatttac ggaaatcatg ctccgcacgc
     9961 aacccgctca acagctggcg gaagggaaca gagagcgcct catatgcggc aataccactg
    10021 gcccagagcg tatcgccgcc ggtcgagggt aactctttcg ccgccagaat cgcccctgcg
    10081 ggtggcgttt gaataaatgt cacatcggtg tgccagttgt cgttatccgg cggattatcg
    10141 ttatgggtat ccaggacgat gatctcgtca accccttcag catgagggta aacagggtga
    10201 atatgcagct cgccaaaacg ctgggccagc gcgcgttgct gctgcggcgt aatagcttga
    10261 tcgcgcagaa acaccacctg atggcgcagc accgcatggt aaagctgttc aaactgatta
    10321 tcgcttaacg ggcgcgttaa gtcggccccc gtaatttgtg cgccaatata cggccccagc
    10381 ggggtaatgc tcagacgttc actcattgta cttctccatg ccagggcgtc aggcggcgct
    10441 gtaacgcgcg cagacccagt tctaaaagaa aggcgataat cgcaatcacc gcgatccccg
    10501 ccagcaccac gtcagtcgcg agaaattcac ccgctgactg aaccataaat cctaaaccgc
    10561 gcgttgcggc aatcagctcc gccgccacca gcgtagacca gcccacaccc agaccaatgc
    10621 gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat gacaaaccac agcacctgcg
    10681 cacggctggc acccagcgac tgggcggcac gaatgcgaac ctgctgcgcg cttttcaccc
    10741 ccgccagcgc cgacatcgcc accggcgcaa aaatcgctaa atagatcagt aagatcttcg
    10801 aggtttcgcc aataccaaac cagatcacca tcagcggcaa ataagccagc ggcggcaccg
    10861 gacgataaag ctcgattatc ggatccagaa tgccgcgcac cgtcgggctt agccccatgg
    10921 cgatcccgac cggaataccg atcaacaccg ccgcgagtag cgccagcaaa atgcgcgtca
    10981 ggctggctgc cagatgctgc cacagcgtgg cgtccataaa tccctgcggc ccggcaatgg
    11041 tgagcagttt tgccagtacc tgttgcggtg gcggtaaaaa cagcgggcta atcagttgca
    11101 gcgccgcgac tgcccaccat acagtgagca aaaccgctaa cgtaccaatg ctgaaggtca
    11161 cctgacgcga gagcggccag cgccatttca gccgatgcga atgcagtttt tcattaatga
    11221 gcacactcat gagaacgcct cccgttgctc aaatacgcgg cttaaaacat attcgcgcat
    11281 ggcgatgaat tgtggatcgg acttgatgct gcggctcgac tctcccgcaa caaagcggcg
    11341 agcaaagttg agcggcagcc gctccagcac acggcctggg ccgggtgaaa gcagaaccag
    11401 ttcagtcgcc ataaacaccg cttcttctat atcgtgggta atcaacagca cctgctttcc
    11461 cgtctcctgc cagagtttca gcagcagggt ttgcatctgg tcgcgggtga aggcgtccag
    11521 cgcaccaaac ggttcgtcga gtaataacag ctggggattt gccgccaacg cgcgggcaat
    11581 ccccacccgc tgacgttgac caccggaaag ctgccagatg tagcgttttt ctgcgccttc
    11641 cagccccact tttttcacca tctggtgtgc gatttccagt cgctgcattt tctctacacc
    11701 tgccagttgc aggccgaaca ccacgttgtc taccacatta cgccacggca acagcccttc
    11761 attctgaaaa accacgcccc gctctgctcc cggtccctca atgcacttac ccgccagttg
    11821 aatgctgcca tgctgataag gcacaaaacc ggcaatcaga ttcagcaagg tggttttgcc
    11881 gcagccagac ggccccagaa ctaccagaag ctcgccgctt tccagcgtca ggttgatatc
    11941 ttccagtgcc ggtttgccgc cataatcggc gtaaagatga gaaatttgca gcatctgcgc
    12001 ctccttttat tgcacgaagc gcgaggtaac gtactggctg taatcattcg ccacggcggg
    12061 gactttgccc tgctctttca aaaactgcgc ggtgtcgatg atcgctttgt tcaccggtcc
    12121 ggtcagttct gccgtttgct gctgcggcgt cagataggta ttccccttca ccagccccgg
    12181 aacgtcacct tcaggcacgc cgcttaaccg cgccagtttg ctgatgtttt ccggctgttt
    12241 cagccacgcg tctgggttag caatgtacgg ttgctgagca tcgatggcgc ttttagcgaa
    12301 cgctttcacg acctcaggat gtttctcggc aaaatctttc cgcacgaccc agacgtccag
    12361 cgtcggcgcg ccccactgcc cgacctgttc agaatcggtc agcaccttgc cgtctttttc
    12421 cagcgcgtta accgccggtg cccagacata agcaccatca atatctcccc gctgccaggc
    12481 ggcgataatt gcgggcggct gcaggttcac aatctccact tgcccaggtt taatacccca
    12541 gtgtttcagc gccgccagca ggctgtagtg ggtggtggag ataaacggta cggcgatgcg
    12601 cttgccaatc agatcttccg gtttgctgat agttttcttc accaccagcg cttcggagtt
    12661 acccagtttt gacgccagta agaagacttc aatcggcacc tgttggctgg ctgccaccgc
    12721 taatgggctg gaaccgaggt taccgatttg cacgtcgcca gaagccagcg cccgcacgat
    12781 gctggctccg ctgtcaaact tacgccagtc aacgctcgcc ccgctctctt tagcaaaggt
    12841 gttgtcggcc tgagccactt tcgccggttc ggctgaagtt tgatacgcca cagtgacgtt
    12901 caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt tacgcgatga
    12961 aattgccatg attgtctgct cccctgtctt gttatgggag cagtattcag gaataaaaac
    13021 attcattaaa agaattagtt gttatcgcac agatgatttt attcttagcg aaaaagtggc
    13081 aaaggaaaga ttaaatatgc aattacaccc actttgacaa ttcagtaagt tactgcactt
    13141 cattttatta atgcataaac aagcagatta taatgtcttt tattttatta acagcaatac
    13201 atataatgaa accattatat tca
//
LOCUS       DAZGAP010000155        13207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGAP010000155 DAZGAP010000000
VERSION     DAZGAP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.156"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..1763
                     /locus_tag="ACHLAM_003926"
     CDS             39..1763
                     /locus_tag="ACHLAM_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO7855382.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARSEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
     gene            1760..2674
                     /locus_tag="ACHLAM_003927"
     CDS             1760..2674
                     /locus_tag="ACHLAM_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7855383.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            2706..2954
                     /locus_tag="ACHLAM_003928"
     CDS             2706..2954
                     /locus_tag="ACHLAM_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7855384.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2954..4126
                     /locus_tag="ACHLAM_003929"
     CDS             2954..4126
                     /locus_tag="ACHLAM_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7855385.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTSYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANNLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4161..5240)
                     /gene="lptG"
                     /locus_tag="ACHLAM_003930"
     CDS             complement(4161..5240)
                     /gene="lptG"
                     /locus_tag="ACHLAM_003930"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7855386.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5237..6307)
                     /gene="lptF"
                     /locus_tag="ACHLAM_003931"
     CDS             complement(5237..6307)
                     /gene="lptF"
                     /locus_tag="ACHLAM_003931"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7855387.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6337..6897)
                     /locus_tag="ACHLAM_003932"
     CDS             complement(6337..6897)
                     /locus_tag="ACHLAM_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7855388.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6909..7733)
                     /locus_tag="ACHLAM_003933"
     CDS             complement(6909..7733)
                     /locus_tag="ACHLAM_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855389.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPV"
     gene            complement(7733..9082)
                     /locus_tag="ACHLAM_003934"
     CDS             complement(7733..9082)
                     /locus_tag="ACHLAM_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO7855390.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9128..9886)
                     /locus_tag="ACHLAM_003935"
     CDS             complement(9128..9886)
                     /locus_tag="ACHLAM_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855391.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9918..10631)
                     /gene="yghS"
                     /locus_tag="ACHLAM_003936"
     CDS             complement(9918..10631)
                     /gene="yghS"
                     /locus_tag="ACHLAM_003936"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7855392.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            10805..11497
                     /gene="yghT"
                     /locus_tag="ACHLAM_003937"
     CDS             10805..11497
                     /gene="yghT"
                     /locus_tag="ACHLAM_003937"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7855393.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11546..13045)
                     /gene="pitB"
                     /locus_tag="ACHLAM_003938"
     CDS             complement(11546..13045)
                     /gene="pitB"
                     /locus_tag="ACHLAM_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO7855394.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            12982..>13207
                     /locus_tag="ACHLAM_003939"
     CDS             12982..>13207
                     /locus_tag="ACHLAM_003939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855395.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYLFLTIKRVLFLVYQTISIHSKSLEIIKT"
BASE COUNT         3356 a         3422 c         3256 g         3173 t
ORIGIN      
        1 tcattttatt ttggatatcc aattttttcg tggtgtatat gtctaataaa atctttacgc
       61 attccctacc tatgcgctat gccgattttc caacgctggt tgatgctttg gactacgccg
      121 ctctgagtag cgccggaatg aatttttatg acagacgttg ccaacttgaa gatcaactgg
      181 aatatcagac gttaaaagcg cgttccgaag ctggtgcgaa aaggttgtta tcgctgaacc
      241 tgaaaaaagg cgatcgcgtg gcgctgattg ccgaaacgag tagcgggttt gtagaggctt
      301 tttttgcctg ccagtatgcc ggcttagtcg ccgtcccgtt ggcgattcca atgggcgtcg
      361 gtcagcggga ttcctggagc gccaaactgc agggtttact ggcaagttgc cagcccgcag
      421 ccattatcac tggtgatgag tggttgccac tggtcaatgc cgcgacgcat aacaacaacc
      481 ccgaattaca tgttttaagc cacgcctggt ttaaggcatt accggaagcc gatgttgcgc
      541 tccagcgtcc agttccaaac gatatcgcct acctccagta cacctccggc agcacccgtt
      601 ttccccgtgg cgtcattatc acccatcacg aagtgatggc taatctacgt gctataagcc
      661 acgatggcat taaattacgc cctggcgacc gctgcgtctc ctggctgcct ttctaccatg
      721 atatgggact ggtcggcttt ctcctgaccc ccgtcgccac gcagctttca gtagattatt
      781 tgcgcactca ggattttgcc atgcgtcctc tgcaatggct taaattgatc agtaaaaatc
      841 gcggcaccgt ttccgttgca ccgccgtttg gctatgaatt gtgccagcgc cgcgtgaatg
      901 aaaaagatct cgctgaactg gatctttcct gctggcgcgt cgctggtatt ggagcagaac
      961 cgatctccgc agaacaactc catcaattcg ctgaatgttt tcgtcaggtt aactttgacg
     1021 ataaaacttt catgccgtgc tacggactgg cagaaaatgc gctggctgtc agcttctctg
     1081 atgaagcctg cggggttgtg gttaacgaag tggatcgcga catcctcgaa taccagggta
     1141 aagccgtcgc gccgggtgct gaaacacgcg ccgtatcgac tttcgtcaac tgcggtaaag
     1201 cgttgccgga acatggcatt gaaatccgca atgaagcaga tatacctgtc gcggaacgtg
     1261 tggtaggcca tatttgcatc tccggcccca gcctgatgag cgggtacttt ggcgaccagg
     1321 tttcgcaaga cgagattgcc gcgacgggct ggttagacac cggcgacctt ggttatctgc
     1381 tggacggtta tctgtatgtc accggacgca ttaaagatct gattattatt cgtggccgta
     1441 atatctggcc gcaggatatt gaatacattg cggaacagga accggaaatt cattctggcg
     1501 atgcgattgc ttttgttacc gcccaggaaa aaatcatttt gcagatccag tgtcggatca
     1561 gcgacgaaga acgtcgcggg cagcttatcc acgcgctggc agctcggatc caaagcgaat
     1621 ttggcgttac cgcggatatc gatctgttgc cgccccacag tattccccga acatcctccg
     1681 gcaagcctgc ccgtgcggaa gcgaaaaaac gttatcagaa ggcttatgct gccagtcttc
     1741 atgtgcagga atccctggca tgaatcaaac tgtcgcggtg acgggcgcaa ccgggtttat
     1801 cggtaaatat attattgata acctgctcgc ccgcggcttt cacgttcgcg cattaacgcg
     1861 tactgcccgc gctcacgtca atgataatct catctgggtg cgcggttcgc tggaagatac
     1921 acattcgctt agcgaactgg ttgccggagc cagcgcggtg gtccattgcg ccggacaagt
     1981 gcgcggacac aaagaagaga tttttacccg ctgtaacgtt gacggcagcc tgcgcctgat
     2041 gcaagcggca aaagagagcg gcttttgcca acgttttctg tttatctctt cgctggcggc
     2101 gcgccatccc gaactctcct ggtacgcaaa ttccaaacac gtcgccgaac aacgactgat
     2161 cgcaatggct gacgaaatta cgctgggcat ttttcgcccg acagccgtgt atggtcccgg
     2221 cgataaagag ttaaaaccgc tgtttgactg gatgctgcgc ggcctgctgc cacgacttgg
     2281 tgcgccagat acacaactct ctttcctgca cgtcaccgat tttgcgcaag cagtgggtca
     2341 gtggttaaac gccgaaactg tacagacgca aacctatgaa ttatgcgatg gcgtcgctgg
     2401 cggctatgac tggcaacgcg tacgacagct tgccgccgaa gcccgttgtg gttctgtgcg
     2461 gatggttggc attcctctgc cgctactcac ctgccttgcg gatatcagta ctgcgttgag
     2521 tcgcctggca ggtaaagaac ctatgctgac ccgctcgaaa attcgtgaat taacccacgc
     2581 cgactggtcg gcaaataata accgtatttc tgaagatatt aattggtttc ccgggattag
     2641 cctggaacac gcattacgca acgggctatt ttgattgtct tgtttaaagg tgtgttaacg
     2701 tttctatggt aaatcgtgaa atagtaatgg attacatcct ttcctgttta caggatttgg
     2761 tcgaaaacgg tgtggaaatt aagcccgaca gcgatctcgt caacgatctt ggcctggagt
     2821 cgatcaaagt gatggatctc ttaatgatgc tggaagacag attcgatatt tccattccta
     2881 ttaatattct gctggatgtc aaaacccctg cgcaattaat ggaaactcta cttccatggc
     2941 tggagaataa ataatggggc tatacgataa atatgcgcgc cttgctggcg agcgcctgca
     3001 atttagtgac aacgggttaa caccatttgg cacctgtatt gatgaagtgt attccgccac
     3061 cgaaggccgg atcggcaata aaaaagtgat tctggccggg accaataatt atctcggttt
     3121 aacctttaat catgatgcta tccgcgaagg tcaggcagcg ttagcggccc aaggcactgg
     3181 caccaccggt tctcgtatgg caaacggcag ttatgcgccg catctggcgt tagagaaaga
     3241 gattgccgag tttttcaatc gccctaccgc tatcgttttt tcaaccagtt ataccgctaa
     3301 tttaggtgtc atcagcgcac ttgccgacca taacgccgtg gtgttactgg atgccgacag
     3361 ccacgccagt atttatgacg cctgttctct cggcggcgca gagattattc gtttccgcca
     3421 taatgacgtc aacgatctgg aacggcgcat ggtccgcctc ggtgaacggg cgaaagaggc
     3481 gattattatc gttgaaggca tctacagtat gctcggtgac gttgcgccgc tggcagaaat
     3541 tgttgatatc aaacgtcgtc tcggtggtta tttgattgtc gatgaagcac attcgtttgg
     3601 cgtgttaggc gcaacaggcc gcggactggt tgaagccgtt ggcgttgaag acgacgtgga
     3661 tattatcgtc ggcaccttca gtaaaagttt ggcttccatt ggtggttttg ccgtgggttc
     3721 agaagcgatg gaagtgctgc gctacggcag ccgcccgtat atttttaccg cctctccttc
     3781 tccgtcctgc attgcgaccg tgcgctcgtc gttaagaacc atttccacac aaccagagct
     3841 gcggcaaaaa ctgatggata acgccaacaa tctgtatgac ggtctgcaaa aactgggtta
     3901 tgagttaagt tcacatatta gcccggtagt gccagtaata atcggttcga aagaggaagg
     3961 tttacgcatc tggcgcgagc ttatttcgct gggcgtttat gtgaatctta tcctgccacc
     4021 tgccgcgcct gcaggcatca cgctgctgcg ctgtagcgta aacgctgccc acagtcgtga
     4081 gcaaatcgat gccatcattc aggcatttgc tactctgaag cagtaatgtg agcccgctgt
     4141 ggtgttgcgc cacggcgggt ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc
     4201 accagcacaa acgctaccgg taaaccgagc gcggtaacag gtgcgctcaa cgcaaataac
     4261 aatcccagat tgaccatgat ttgatagctg atccaggtga gtaagccaac gatgacgcct
     4321 acagcgagac ggctacccat cccaggcgaa cgcggggcgc taaaggtaaa cggcacagcc
     4381 agcaaaatca tcgccagggt caagatgggt tgtcccagtt tttcccacag tgcaaggcgg
     4441 tattcgctgc tgggttgccc ggtttcctgc aaataatgga tgtaatgatt aagttgacgg
     4501 acagaaaaag tatttcccgg catcgataac tcttcaagat tcatactggt gaaggccgat
     4561 tgccagggaa gattatcaga tgtttcctgc gtttctttgc cattcagcca ttttttatga
     4621 ttcacaccat gcaggatcca cgttttgtcg tcttcaatgg tagcactgcg cgcataaatg
     4681 taggattcga gcgagagatc atcgcggtaa tgaaatatct ccacgtccac cggctggccc
     4741 tgctcgttca gcgatttcac cgtcacaaat tcattaccac gcctggccca cagcatattg
     4801 ccggtaatgt catcgtcctc ccccaacgca gtggcagtcg atttgatttg cagcgcctgt
     4861 tgctgcaatg gcgacgccac ccactcatct atcgcgccta atgaaacagt ccacaatatc
     4921 ccggcaacca gcgccaccag tgcaatacgg aagatagaaa accccgtgct gcgaatggcg
     4981 gtaagctcac tgtttttcga taactgcccc aggccgacaa tccctcccaa taaggcgata
     5041 aacggcgaaa gttcgaccag tgtgcgcggt aaggtcatca gcaccaccag caccgcctga
     5101 gtccagcgat aaccacccgg gctgacatca tccagttcgt taatcaggtt gaaggtggta
     5161 aaaagcggca gcaatagccc tgcggcggcg gcaaaaccga gaaagagatg acggattaaa
     5221 tagcgactga aaacattcat cgggaaaatt tctgcagcaa agagaagtcg cgtgcgacca
     5281 acatcagcag gcccattagc atgagtccgg gtactaacca caaaccagga atgaggggaa
     5341 tcgcgccgtt agccaccagc gtacggcaga cgtcgccgcc ataaaaaatg gcaacaaaca
     5401 gcaacgtcag tggcaataac gttgaaaatc gcccttgccg cggttttacc cgacttaatg
     5461 aaatggccag cagcgccatc aacaatgctg tcaggccacg gctttgtcgc cattgcagtt
     5521 cggcatgatc ggcaggaaag gcggagcgcg ccagctccgt gaccgatttt gctttgcgtt
     5581 tgacgttagg gctttgatcc agcggattca gatgtaattg cagattacgg tagatctgtt
     5641 cgttgtcgtc acgcccctga tgatcgagaa gataggcggt cccgttatgc aacataacgg
     5701 tcggtttctc aggtgatggg tcaaccacat caaccgaacg ggcgcggaaa atgcgggttc
     5761 gattagcagt agaagtataa atcagcgcat cagtcaggcg attattatcc tgatcaaccg
     5821 tctgcgaaag gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc
     5881 gaacatccag ctccgactgt gactgttgct ccagttgata aatctgcgca taagcccacg
     5941 gacgaccata catcgacaga agggtgacaa aaatacttag cggtatcgcc agataaagaa
     6001 cggctttgta caagcgtccc ggactgcccc ccgccgcaga gatagcggta atttccgagt
     6061 cggtatacat ctgccctagc gtcacgccaa ctgacacata cagtccaaca ggtaacaaca
     6121 tctccagtgc aatcagcact ttgtagaaaa cgatatccag cacaacatca agagctaatg
     6181 tcccatttgc cgcttcggtc agataacgct gtgcggagta gctggcgaaa ataaagatca
     6241 gaaaaccgac gataatcagc accagacggc gcgtaccacg catgatgtag tgttcaacca
     6301 gcttcatctc actaacgccc tcaatccttt gaatttttat tcattgatca gcttctgcgc
     6361 cagcgcaatg acatgatcta cctcttccgg cgtcaggctg ccgtcctttg cccactgaat
     6421 ttgcccggtt ttattcagca ccagaatggt tgaactctgc tcctgcaagt gccaggcaac
     6481 gcgtccctgt ccattgccgt caataacgaa ttgcgcccat ggatagcggc gtttattttt
     6541 ttcgattttg ccaacgacaa aatagccggt accaaagatg gcatcatcag tgttcacaat
     6601 ggtggtgggc tgaaaacgat cctgcggaaa gtttgccgct tcaactgctt taattaacag
     6661 tgagttcttt ttcttcgccg acttacgccc ggcgatatac tgaataatgc gaacttttcc
     6721 cgccaattgg gtgctgttcc acttttggta gtgaatttcg tcgtcgctat tccgaagcag
     6781 ctcgccgcct tcctggatat aaacgggggt aaccgttttg ccagtgacga aattgtgtgc
     6841 ctgcacaccc ggggctaaga gcatgataat cagcgcaata atcaaacgag atgacatcat
     6901 tgctccggtt ataccggaaa acgcgaaatc agcagttcta agcgctggcg aatatcgcta
     6961 tccagcgcca tcggtgcatc atacgactgg cggttttcca tatcatccgc caccatgtcg
     7021 tcgtgtttat cccccatcag gccattaatg gcggcgagga agcgggaaat ctgtaacgct
     7081 tctttatttt caggatcaag cgatcttagg gtgtcgtaat aagaaacctg ctggctgatg
     7141 atttttcgca catcatgaaa ttccctgccg gttagtcgcg gtttggcaat tatctctttc
     7201 agggtatgca tttgcttttg ccgataggca ataaaaccgc ttacgctttc taactggcta
     7261 tggcgtaatg aatagttcac caacgaccag accggagagc ttaaataaac ccgtaacagg
     7321 ttgccgtaat acgacacaat atttttctta ccgttacgaa agccgtcctg aaaacgcccc
     7381 aaaaaaacgg tcgttttgcc gaataaaaat ccggcctgat gcttctttaa atacagccgt
     7441 tgcgcaaaac gcaggtgctt gtagcgcgct cgcatgtatt taaattgttt gcgctcatca
     7501 gcagtcagct catcgccttt ggcctgtttg catattaaac gcagtaactc ctggcgattg
     7561 acgccattga gccagaattg caggcataac gcgtaattat catggatgat ttcggtggta
     7621 caacttagtg cgatagtcgg cggcaaactg acctgtgcat caacgacatc atcttcttca
     7681 atcgcaacaa atagacgctc gctcgcctct agcgggaaat attttctcat catcaggatt
     7741 tctctccggc tgcgttataa agcaaggaat ggcgctgaaa aaatgccttt aaggtcggaa
     7801 ttagcgaaag caccacatcc agacattgac caccgaaata agcaaacgct gctcccagca
     7861 cgccaaacag atgacagaac atgaacagca atacgatgta gccgattgag gcgatggttt
     7921 gcgccacgag aaaagcgcgc tgttttcccg ccatcaatag caatgactcc tgagggaagc
     7981 ccagcatcga gatcacaatt gcgcccagca tcacctgaat caggtcatac gcttcgagat
     8041 acttaacgcc aaacaccagc gaaatgagcg gcttgccgac aatcaacacc gcgagcgcca
     8101 ccagaatacc gattccgccc gccagtaaac ctgacttcac gcctaacaac cacggtctgg
     8161 tggtacgcgg atctaaacgc atcacctctg ggtaaaaact tttgcccagt aaacccgctg
     8221 gcgttccggc ggcgtcgaag aatgtcatgg cgattttaaa taacccggcg gcagcgggtc
     8281 ctaacacgat ccccaccaac actgtgctgc acgaattacg cgccgaccag atggaatggg
     8341 caatgttggt tgaccagaca aaactccacg cgcctttgat gtgtcgggca gactcaaaca
     8401 gattcaattt gaacgcgtta tggatatttc tgcggcgtaa ttcacgtgcg gcaaaccacc
     8461 agtacatggt gccgccaaca agattcgagg cgtaccaggc aataacaaaa cccgcaaaac
     8521 caaagtcaaa ataccaggca acgacgctcc ccgctgcgcg cagaaaaggt ttcgtcgctt
     8581 gctgtacagc aattagatcg aagcgatcta ccgcacgcag aatgccggtc ggcgtggagg
     8641 aagccattga aggaatgagc gtgcaataca gcgctgccag ccaaaagctt tggtcatcca
     8701 gacctaatga atgggaaaga aaagggagta aggcaatgcc gccgacaatc gccaccgcgc
     8761 cgctgacgat atccagcgag aaggaaaatg agacgacatt gcggaattgc tgcggattat
     8821 tgttggttaa tgctggtgtt ccgtactgaa ccaccagctg ccatgtctga aacttaataa
     8881 aatcgctgat cgacttggcg tacgattgta caatcaccag tacgccaaac atggcgggcg
     8941 tcatcccttt accggcacac gagagcgcca acagacccag caaagcgctc acgacattac
     9001 tggagcctaa ccaggcgcta ttgcgaataa tggtgcgaaa cgcgccatct gcaaaccaat
     9061 gtttgatgtt aaaacccgcc aattcagcct gaccttatcg ttgatagtaa aaagtatccc
     9121 gccagcctta agttaaactt cggcggtcag aaacgatggc aaccagagaa accgccttct
     9181 gtgcctgttc cagcacttcg ctgtagggcg ctctggaatc aatctcaaga atttttgtgc
     9241 cgttatagcc aatcgtcgac atgacgccga ttttgtcctg cagctcggca tagtcatggt
     9301 caggcttgcg ggagatggca gtatcaatat caatccccaa acgaatgatt aattcaggac
     9361 ggtattgcgc catttgttgg tataaacgcc gttcgcgctg cgccagaaac atgctgattt
     9421 tcccggtcgc acgttcgacg ccgatccccg gtccgtcata ataaaagccc gaaatttcag
     9481 cctgcgggaa gcgatcgctg accaccagaa cgccactttg cgccagtcgc tgaaccttgc
     9541 gtagattcgc cattcttcgc agcgagaagc agtacataat caccgccgcc catagcgctg
     9601 gagatttggt tttcatgctt tgggttttcg aggatttggc cgccagtcgc cgttcgagcc
     9661 agacgccaac caacggcaat cgtttgattt tgtcgccgtc ttcgcccgag agcagcccca
     9721 gatagcgccg ctcggtttgc cagtgttgtt gcagcgattt caccaggtcg gtggtcagtg
     9781 tggatttacc ggtgccatca cacccgacca ccgcaatcag ccccggaatg tagttgggct
     9841 gcggcgcggt tgtactatta acagtttgtg tttgtagtgc gtccattcgt taaatcatcc
     9901 cctatccatt ttggtgatca ggaaagcgcg gcgtgaattg cgcgtagtga cgcttgcaga
     9961 atttcatcgg cgggttgccg accatccagt tcaaggatct ttgcgccatt aaatgtcagt
    10021 tgcggcgtaa cggcgatttt ttcctgtaac gccgccagtt ggtggtcagg tttacgcgca
    10081 aacgcggttt gttcatcaat gccaagacga atcaacaata cgggcaaata agacgccatc
    10141 cattggtaca gcttcagctc gcgctgcctt aacattttta tccaaccgtt accgcccgtg
    10201 gtttttgcca attgcgggcc atcaaagcga aaacccggca cttcaacctg cgggtagcgg
    10261 tcggtgatga gcagatagcc ttgctggctt ttacacaaca ttttgcgaaa cttgtacgcc
    10321 cgccagcagg aaagcagata gataactagc gcagtaatat tgccaggtgg tgtggagggc
    10381 ttttcgtgca catgtgccgc tttacttcgc agataacgcc caaaaggtgc gccaataaca
    10441 gggagctgtg aaatccattc gccaattcgc ccggacgatt gcccgagata aatgtgttct
    10501 gttggcattg ttgctgccag ttcatttacc aggcttgccg tgagggtcga tttacctgaa
    10561 ccatcacatc ctacaatggc aataacacgc actggcgtgg aattaattat tgacatatta
    10621 cgttgattca cgaaaaaccc ggcagcaaat aatgtattga atattacgtg gtccgtgctc
    10681 agaatatccg ttcaacctta ttgtgacaat ggttaattgt gacattaacc tgaattacgc
    10741 tttcataaaa acatattaat caaataaata ttttggatgg atatttaaat ttaaggatat
    10801 attcatgcag tcaataacac ctccattaat tgccgttatt ggtagcgatg gttcaggcaa
    10861 gtcaacggtg tgtgaacatc ttattaccgt tgtcgaaaaa tatggtgctg ccgaaagagt
    10921 tcatttagga aaacaggccg gaaatgtcgg tcgtgcagtg acaaaattac cgttgatggg
    10981 aaagtctctg cacaagacta ttgaaaaaaa tgaagtgaaa acggcaaaaa aattgccggg
    11041 tccggttccg gccctggtga tcaccgcttt cgtcgcccgc cgactgttac gctttcgtca
    11101 tatgctcgcc tgtcgtcgtc gcgggctgat tgtcttaacc gatcgttatc ctcaggacca
    11161 aattcctggc gcttacgatg gtacggtgtt cccacctaac gttgaaggcg gccggtttgt
    11221 ctcatggctg gcaagccagg aacgtaaagc atttcactgg atggcgagcc ataagcctga
    11281 tctggtcatc aaactcaatg ttgaccttga agttgcctgc gcgcgtaaac ccgaccataa
    11341 acgggaatcg ctggcgagga agattgccat aacgccacag ttaacctttg gcggtgcaca
    11401 actggttgat atcgacgcca atcagccact ggaaaaggtg ctggttgatg cagaaaaagc
    11461 gattacggat tttatgaccg cgcgtggtta tcactagtca aaaatggaaa tgcccgatcg
    11521 ccaggaccgg gcattttcag gaaggttaaa tcaactgcaa tgctatccag tacaacccac
    11581 cagaaagaaa aatcgccgcc ggtaaagtaa acacccacgc catcaggatg ctggttaccg
    11641 ttttacgctg taacccaccg ccgtccacca ccatcgtccc tgctaccgca gacgagagga
    11701 cgtgtgttgt ggagacgggc atcccaatat aactggcaag gccgatagac actgccgccg
    11761 tcatttgtgc cgccatgcct tgcgcatacg tcatgccgcg cttaccaatc ttctcaccga
    11821 tggtcatcgc tacacgacgc cagccaatca tggtgccaat gccgagcgcc agagctactg
    11881 ccatgatgat ccacaccgga gcgtactcaa tggtgcttaa catatcgctg cgcagttttt
    11941 tcagcaggtt ctggtcttct ttactgacgc ctggcagttt cgccagcttc gcggaggtat
    12001 cagagatgca cagcataatg cggcgcagct ggctgcgctg actcacgctt aacggctcgt
    12061 aactttccat attgcctggc agcatcgttt taacgcgcgc aatcgcatca aaggtatttg
    12121 ccggatgaca gtgaaactcc gttacttgcg tgccatcagt cgatgccgca ggcaatggag
    12181 gttccatcgc aatcaacttc tgcggcagtt caggatgctg ttgcaggtag tgttcgaagt
    12241 tggtgacggc atcgcgggta cgggtaattt catagccgga cgcattcata ttgacgacga
    12301 aaccagcagg ggcaatcccc accagtacca gcatcaccag gccgatccct ttctgcccgt
    12361 cgttagcacc gtgcgaaaac gccacgcccg cagcggaaac aatcagcgca atacgcgtcc
    12421 agaatggtgg cttacgtttg ccttttttct ttttgcgatc ttccggaatg cggtgaatac
    12481 ggtcacgctt tttcgtcccg ctccagaaac ggcgcagcag gaaaatcaga ccacccgcaa
    12541 tgaccaggcc gacgataggg gaaacaatca gcgaggagaa aattttggtc acttcacgca
    12601 ggtttaacgc atccatcacc gatgagccgg ttaacagcgc gttggttaaa ccgatgccga
    12661 taatcgcacc aatcaaggtg tgcgaactgg aggccggtaa accaaagaac cacgttccca
    12721 ggttccagat aatcgcggcc agcagcatgg aaaagaccat cgccaggccg tgagttgacc
    12781 ccatattcag caacaaatcg gttggtaaca tatggacaat ggcataggca acgctaagtc
    12841 cgcctagcaa tacgccaaaa aagttaaaaa atgccgccat caccacggca agctgtggct
    12901 gcatggctcg agtataaata acggttgcca ccgcattcgc cgtgtcatga aaaccattga
    12961 ttgcttcgta gaacaacaca aatgccagag caagtaataa caaaagccct gtgtatatat
    13021 caaggccaac aaataaattt agcatatatt agattacgcc attttgaatt tacgaacgga
    13081 cgcattatca gtgactttaa cggcatgggc aaagtgaaat atcttttttt aacgataaaa
    13141 agagtgttat ttcttgtgta tcagacaata agcattcatt caaaatcttt agaaataatc
    13201 aaaacac
//
LOCUS       DAZGAP010000156        13122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGAP010000156 DAZGAP010000000
VERSION     DAZGAP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.157"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(186..1298)
                     /gene="ydiK"
                     /locus_tag="ACHLAM_003940"
     CDS             complement(186..1298)
                     /gene="ydiK"
                     /locus_tag="ACHLAM_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO7855396.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            1685..4741
                     /locus_tag="ACHLAM_003941"
     CDS             1685..4741
                     /locus_tag="ACHLAM_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO7855397.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSSMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWVAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4738..5148
                     /gene="menI"
                     /locus_tag="ACHLAM_003942"
     CDS             4738..5148
                     /gene="menI"
                     /locus_tag="ACHLAM_003942"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO7855398.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5248..5436
                     /locus_tag="ACHLAM_003943"
     CDS             5248..5436
                     /locus_tag="ACHLAM_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO7855399.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            5985..6353
                     /gene="sufA"
                     /locus_tag="ACHLAM_003944"
     CDS             5985..6353
                     /gene="sufA"
                     /locus_tag="ACHLAM_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO7855400.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6362..7849
                     /gene="sufB"
                     /locus_tag="ACHLAM_003945"
     CDS             6362..7849
                     /gene="sufB"
                     /locus_tag="ACHLAM_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO7855401.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7859..8605
                     /gene="sufC"
                     /locus_tag="ACHLAM_003946"
     CDS             7859..8605
                     /gene="sufC"
                     /locus_tag="ACHLAM_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO7855402.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8580..9851
                     /gene="sufD"
                     /locus_tag="ACHLAM_003947"
     CDS             8580..9851
                     /gene="sufD"
                     /locus_tag="ACHLAM_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO7855403.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9848..11068
                     /gene="sufS"
                     /locus_tag="ACHLAM_003948"
     CDS             9848..11068
                     /gene="sufS"
                     /locus_tag="ACHLAM_003948"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO7855404.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11081..11497
                     /gene="sufE"
                     /locus_tag="ACHLAM_003949"
     CDS             11081..11497
                     /gene="sufE"
                     /locus_tag="ACHLAM_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO7855405.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11646..12650
                     /gene="ldtE"
                     /locus_tag="ACHLAM_003950"
     CDS             11646..12650
                     /gene="ldtE"
                     /locus_tag="ACHLAM_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO7855406.1"
                     /translation="MKCASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12714..12950)
                     /gene="lpp"
                     /locus_tag="ACHLAM_003951"
     CDS             complement(12714..12950)
                     /gene="lpp"
                     /locus_tag="ACHLAM_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO7855407.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3304 a         3220 c         3515 g         3083 t
ORIGIN      
        1 gagctaatag taggcatacg gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa
       61 actgtaaaga atttgcgaca ggtactacca gctgaatggc ggtaagcagg aaaatatggg
      121 gaagagcaag caaatatcaa ttagcagggg ttaacaatca ggcggcagag ccgcctgatg
      181 agaaattact tattcgattt ctcgatttcg ccaagttctt cgagaatttc ttccggttgg
      241 tccgtcggcg gtgggacttc ttccacccac gcggcaaaaa gacgccagga aacggctaac
      301 agaaccggac caataaacag gccgatcatc ccgaaagcaa tcaaaccacc aataacacca
      361 gagaggatca ggatcagcgg taaatcggca cccatgcgaa ttaacattgg gcggatgacg
      421 ttatccagcg tgccaaccac accgctccac actaacaata ccgttcccca ggtggtatca
      481 ccggtccagt agagccagat aatcgccgga atcagtaccg gcaacggtcc aagctggaca
      541 aggcaggaga ggatcattaa caccgtgagc aaagttgcat aaggtacgcc ggatacggcg
      601 aggccgatac cgccaagcac tgcctgtact aacgccgtca ccaccacacc cagcgccacc
      661 gcgcggatag cctgtgccgc cagtagcaca gcagcatcac cgcgaacgcc tgccagacgg
      721 gttgcaaaat ggcgaatgcc ttgtgccacc tgttcaccgc gccagtacag cagggcactg
      781 aagagaagca tcagcgcaca atgcaccata aagcgcccga tatgcgccgc ctgcccaacg
      841 aaccaggtgg tggtggtgcc aatataagga cggactttcg ccatgatcgc cgtacccccc
      901 atatccagca agttgtgcca gcctgcatat agcttcgcgc caatcaccgg aatggtatta
      961 agccacgcca gatcgggtaa cgtcatgtca ccgctggaaa tggctttaat tagcggaccg
     1021 ctgccatcga caatactgtt aaccaacaag gcaataggga tgataaacac catcactaat
     1081 aacagcgtca tcaccagaac ggcgagagag cggcggccaa acatgatctt ttgcaaacgt
     1141 aacaataccg gccaggtggc gataaccact gtaccggccc atgcaaagcc gagaataaag
     1201 ggttgaacaa tccacagaca tgccacaatc atgatggcta aaaacagcac cgaaagcaga
     1261 atttgtgcga catccctggg ctgacgaaca tttaccataa ctacttttca ccttttttgt
     1321 gcgtcaaatc gtcgacgcga cgtgaacacg cgaaactcac ctgcataatg atgagcaatt
     1381 tcagcggttt ttaacaggtc gattctgccc ttaattctga tgggcgtgta agaaaaaaat
     1441 gtggtacaac aactcaggca acacgcaaac gatttactcg tcgtatttca aattgcatgt
     1501 gctgcggcta cgttcgttca ccccagtcac ttacttttgt aagctcctgg agttcactcg
     1561 cttgccgcct tcctgcaact cgaaatccat agagtaaaca aacatcacct ttcagtgcgc
     1621 tattagcagc ctgaaagaac agtgtatcga tctacatcac agaccgcagg aaagggtcaa
     1681 tataatgatt ccacagattt cccaggcacc cggcgtcgtt caattggtgc ttaatttttt
     1741 gcaagagctg gagcaacagg gttttactgg cgatacggcg acaagttatg ccgatcgtct
     1801 gacaatgtcg accgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg
     1861 ttcaaccgca gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct
     1921 gatctttacc ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat
     1981 tattgttgat atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg
     2041 ggtgcgcgtt gaggccgggg tgataaaaga tcaactcaat cagtacctga aaccgttcgg
     2101 ctactttttt gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa
     2161 taccgatgca tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg
     2221 cgtacgcgcg gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgaact
     2281 ggcggaaacg ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca
     2341 acgttgccgt cagcaacgcc agttaatcat cgacaacttc cccaagctta accgctttct
     2401 taccggttac gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg
     2461 cattctgacg ggttcagaag ggacgctggc ctttattacc gaagcgcgac tggatattac
     2521 gcgcttgcct aaagtgcgcc gtctggtgaa cgtcaaatat gactcttttg actccgcact
     2581 gcgtaacgcg ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa
     2641 agtgctgaat ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga
     2701 tgtgcctgac aaagaaatgc tcgggctgaa cattgtggaa tttgctggtg atgatgagac
     2761 gctgattgat gagcgggtaa atgcactctg tgcgcggttg gatgaactga ttgccagcca
     2821 acaggcaggt gtgattggct ggcaggtgtg ccgcgagctg gcgggtgttg aacgtatcta
     2881 tgcgatgcgc aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat
     2941 tccgtttgct gaggatacct gcgtaccgcc ggaacatctg gccgattata ttgctgaatt
     3001 tcgcgcgctg ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg
     3061 tgtcttgcac gtccgtcctg cgctggatat gtgcgatccg caacaagaga ttttgatgaa
     3121 gcaaatctct gatgacgtag tggcgcttac cgcgaaatac ggtggtttgt tgtggggcga
     3181 acacggcaaa ggttttcgtg ccgaatacag ccctgcgttt ttcggtgagg aactttttgc
     3241 agaactgcgc aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat
     3301 ttgcccgcca gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac
     3361 attcgatcgg cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa
     3421 cggcaacggt ttatgcttca actttgatgc ccgtagttcg atgtgtccgt cgatgaagat
     3481 cacccagaac cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg
     3541 cttgttggcg gatcgcggcg ttgacccact caaactggaa caagaactgc ctgaatccgg
     3601 cgtcagtttg cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga
     3661 atacgacttc tcacacgaag tcaaagaggc gatgtcgggc tgtcttgcct gtaaagcgtg
     3721 ttcgacccag tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct
     3781 ctatcacacc cgttatttac gcccgcttcg tgaccacctc gttgctacgg tcgagagcta
     3841 cgcaccgctg atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt
     3901 gcgcaagctc tcggaaaaac atatcggcat ggttgatttg ccactgctgt cagtcccctc
     3961 gctacaacaa caaatggtgg ggcatcgctc ggcgaatatg acgctggaac agcttgaagc
     4021 tctcaatgca gagcagaaag cgcgcacggt gttggtggtg caggacccct ttaccagcta
     4081 ttacgatgcg caagtggtgg cggattttgt ccgtctggtt gaaaaattag gtttccagcc
     4141 tgtgttactg ccattttcgc caaatggcaa agcccagcat atcaaaggct ttcttaatcg
     4201 ttttgcgaag acggcgaaaa agacggcgga tttcctcaac cgtatggcga agctggggat
     4261 gccaatggtg ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc
     4321 gctgggcgag gaacgtggcg agtttaacgt cttactggcg aatgaatggc tggcaagcgc
     4381 acttgactca cagccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg
     4441 taccgaagtt accgctttgc cgggcgcgcc agcacaatgg gttgcgatat ttgcccgttt
     4501 tggcgcgaaa ctggaaaatg tcagcgtggg gtgctgcggc atggcaggga cttacggaca
     4561 tgaagcgaaa aaccataaaa attcgctcgg gatctatgag ttatcctggc atcaggcgat
     4621 gcagcgactg ccgcgtaatc gctgtctggc gaccggatat tcctgccgta gccaggtaaa
     4681 acgggtcgaa ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg
     4741 atatggaaac gaaaaatcac cctggaagca ctgaatgcca tgggtgaagg aaacatggtg
     4801 gggttgctgg atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca
     4861 gtagactcgc ggacaaagca gcctttcggg ttgctgcatg gaggggcatc cgtggtactg
     4921 gccgaaagta tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaggtggtt
     4981 ggtctggaaa tcaatgctaa ccacgtccgc tcggcacgag aagggcgggt gcgcggcgta
     5041 tgcaaaccgt tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag
     5101 aaagggcgtt tgtgctgttc gtcacgatta acgaccgcca ttttgtgacg ttgtcaccag
     5161 aaaagtgtga cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg
     5221 caggtcttgt ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc
     5281 attgaatttt tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg
     5341 aaacaactag aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa
     5401 gaaatctact ttgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaattttg
     5461 ccaaatcgac ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct
     5521 atccgcctga tattgttcaa aaataatact aatcgcagtt tgtgttaaaa cggcgggtta
     5581 gctttatgag attattctta tcgccccttc aagagctaag ccactgtgag tgccggagat
     5641 aagcgccgga tggggtagaa acccttaagc ctgtgtcgca cagacttaag ggttttctta
     5701 tttctgaacg ctgcactttt ttacctatcc cttaaatagt cattttcaat acaacttttt
     5761 ctttttcatc acagccttaa tgctccttag ataataatca ttatctaaca atgagatacc
     5821 taattcttag cgtgcctgtt aacccacaca tcagggtcta tgcttattaa aagcaggtaa
     5881 aaagcatgtt ttacgctaaa gtccctgcgt ttgctgggtt gaactgataa tcattatcac
     5941 taacatgctg ttatacgctg aaagcgatga agtgaggtaa atcgatggac atgcattcag
     6001 gaacctttaa tccacaggat ttcgcctggc agggcttaac gctgacaccc gcagccgctg
     6061 tccacatccg cgagctggtg gcaaagcagc cgggtatggt cggcgtgcgc ttaggcgtga
     6121 agcaaacggg ctgcgcgggc tttggctatg tgctcgacag tgttagcgag ccggacaaag
     6181 acgatctgct gtttgaacac gacggcgcga agctgtttgt cccgctgcaa gcgatgccgt
     6241 ttattgatgg cacggaagtc gatttcgttc gtgaaggact taatcagata ttcaaatttc
     6301 acaaccctaa agcccagaat gaatgtggct gtggcgaaag ctttggggta taggcggtac
     6361 tatgtctcgt aatactgaag caactgacga tgtcaaaacc tggaccggcg gcccgctgaa
     6421 ttataaagaa ggattcttca cccagttagc caccgatgag ctggcaaagg ggataaacga
     6481 agaggtggtg cgcgcaattt cggcgaagcg taatgagccg gagtggatgc tggagtttcg
     6541 tctaaatgcc tatcgcgcat ggctggagat ggaagaaccg cactggttga aagcgcacta
     6601 cgacaagctg aattatcagg attacagcta ctattcggca ccatcgtgcg gtaattgtga
     6661 cgacacttgt gcgtctgaac ccggcgcggt gcagcaaact ggcgcgaacg cctttttaag
     6721 taaagaggtg gaggcggcgt ttgagcagtt gggcgttccc gtgcgggaag gcaaagaggt
     6781 ggcagtggat gccattttcg actctgtttc ggttgccact acttatcgcg aaaaactggc
     6841 ggagcaggga attatttttt gttcctttgg cgaggcgatc cacgatcacc cggaactggt
     6901 gcgtaaatat ctcggcaccg tggtgccggg gaatgacaac ttctttgccg cacttaatgc
     6961 ggcggtagcc tctgacggta cgtttattta tgtgcctaaa ggcgtgcgtt gcccgatgga
     7021 actttccacc tattttcgca ttaacgcgga aaaaaccggg cagtttgagc gcaccattct
     7081 ggtggccgac gaagacagct acgttagcta cattgaaggc tgttccgctc cggtgcgtga
     7141 cagctatcag ttacacgcgg cggtggtgga agtcatcatc cataaaaacg ccgaggtgaa
     7201 atattccacg gtacaaaact ggttccctgg cgataacaac accggcggta ttctcaactt
     7261 cgtcaccaag cgtgcgttgt gcgaaggcga aaacagcaaa atgtcatgga cgcaatcaga
     7321 aaccgggtca gcgattacgt ggaaatatcc cagctgcatt ttgcgcggcg ataactccat
     7381 tggtgagttt tactcagtgg cgctgaccag cggtcatcag caagcggata ccggcaccaa
     7441 gatgatccac atcggtaaaa acaccaaatc gaccattatc tcgaaaggga tctctgccgg
     7501 acatagtcag aacagttatc gcggcttagt gaaaatcatg ccgacggcaa ccaatgcgcg
     7561 caatttcact cagtgcgatt caatgctgat tggcgctaat tgtggggcgc ataccttccc
     7621 gtatgtcgaa tgtcgcaata acagcgctca gctggagcac gaggcaacga catcacgtat
     7681 tggtgaagat caactgtttt actgcctgca acgcgggatc agcgaagaag atgccatctc
     7741 gatgattgtt aacggttttt gcaaagacgt gttctcggaa ctgccgctgg aatttgccgt
     7801 tgaagcacaa aaactcctcg ccatcagtct tgaacacagc gtcggataag gaataaacat
     7861 gttaagtatt aaagatttac acgtcagcgt ggaagataaa gctatcctgc gtggattaag
     7921 cctcgacgtt cgtcccggcg aagttcacgc cattatgggg ccaaacggtt ctggcaaaag
     7981 taccttatcg gcaacgcttg ccgggcgaga agattatgag gtgacgggcg gcacggttga
     8041 gttcaaaggc aaagatttgc ttgcgctgtc accggaagat cgtgcaggtg aaggcatctt
     8101 tatggccttc cagtatccgg tggaaattcc cggcgtcagt aaccagtttt tcctgcaaac
     8161 ggcacttaat gcggtgcgca gctatcgcgg ccaggaaacg ctcgaccgct ttgattttca
     8221 ggatttgatg gaagagaaaa tcgctctcct gaagatgccg gaagatttat taacccgttc
     8281 ggtaaacgtt ggtttttccg gcggtgagaa aaagcgcaac gatattttgc aaatggcggt
     8341 gctggaaccg gagttatgca ttcttgatga gtcagactcc gggctggata ttgacgcatt
     8401 aaaagtggtt gccgatggcg tgaactcgct gcgtgacggc aagcgttcat tcatcattgt
     8461 tacgcactac caacgcattc tcgactacat caagcctgat tacgtccatg tgctgtatca
     8521 gggacgaatt gtgaaatccg gcgatttcac gttggtcaaa caactggagg agcagggtta
     8581 tggctggctt accgaacagc agtaacgcac tgcaacagtg gcatcatttg tttgaagctg
     8641 aaggggcgaa acgctccccg caagcacagc agcatttaca acaattgctg cgtaccggac
     8701 tgccgacacg taaacatgaa aactggaaat atacgccgct ggacgggctg accaatagcc
     8761 agtttgtcag cattgcgggg gagatatccc cacagcagcg tgatgcctta gcgttaacat
     8821 tagacgccgt gcggctggtg tttgtcgatg gacgttacgt gcccgcactg agcgatgcaa
     8881 ctgaaggcag cggatatgaa gtgagcatta acgacgaccg tcaggggtta cccgatgcta
     8941 ttcaggcgga agtgtttctg catttgacgg aaagcctggc acaaagcgtg acgcatatcg
     9001 ccgtgaagcg cggtcaacgg ccggcaaagc cattgctgtt aatgcatatc acccagggcg
     9061 tggcaggtga agaggtgaat actgcccatt accggcatca tctggatctg gcggaaggtg
     9121 ccgaagcaac ggtgatcgaa cattttgtca gccttaatga tgctcgtcac ttcaccggcg
     9181 cacggttcac tatcaacgtc gcagcgaacg cccatttgca gcatatcaag ctggcgtttg
     9241 aaaacccgct cagtcaccac tttgctcata acgatttgtt gctggctgac gatgccaccg
     9301 catttagcca cagtttcctg ctgggtggcg cagtattacg acacaacacc agtacgcaac
     9361 tcaatggcga aaacagcacg ctgcggatca atagcctggc gatgccggtg aaaaacgagg
     9421 tgtgtgatac tcgtacctgg ttggaacaca ataaaggttt ttgtaacagc cgacagttgc
     9481 acaaaactat cgtcagcgac aaaggccgcg cggtatttaa cggtttgatc aacgtcgcgc
     9541 agcacgccat caaaacggat ggtcagatga ccaacaataa tctgctgatg ggcaaactgg
     9601 cggaagtgga tacgaaaccg cagctggaaa tctatgcaga tgatgtgaaa tgcagccacg
     9661 gcgcgacggt ggggcgtatt gatgacgaac agatgtttta tctgcgctcg cgcgggatca
     9721 atcagcaaga tgcccagcag atgatcattt acgcctttgc tgccgaactg acggaagcac
     9781 tgcgtgatga ggggcttaaa cagcaggtgc tggcccgaat cggtcaacga ctgccaggag
     9841 gtgcaagatg actttttccg tcgacaaagt gcgggccgac tttccggtgc tttctcgtga
     9901 ggtaaacggt ttgccgctgg cttatctcga cagcgccgcc agtgcgcaga aaccgagcca
     9961 ggtgattgat gccgaggctg agttttatcg tcatggctac gcggcggtgc atcgcggtat
    10021 tcatacctta agcgcccagg cgaccgagaa aatggagaac gtgcgcaagc gggcatcgct
    10081 gtttattaat gcccgttcgg cggaagagct ggtgttcgtc cgcggcacga cggaagggat
    10141 caatctggtc gccaatagct ggggcaacag caatgtgcga gcgggcgata acatcatcat
    10201 cagtcagatg gagcaccacg ctaacattgt cccctggcag atgctttgtg cacgcgttgg
    10261 cgcagagctg cgtgtgatcc cgctcaaccc cgacggtact ctgcaactgg agacgctgcc
    10321 tacgctgttt gatgagaaaa ctcgtctgct ggcaattact catgtctcca acgtgcttgg
    10381 gacagaaaat ccgctggcgg agatgatcac acttgcgcat caacatggcg caaaagtgct
    10441 ggtggatggc gctcaggcgg tgatgcatca tccggtggat gttcaggcgc tggattgcga
    10501 tttttacgtg ttctccgggc ataaactgta tggccctacc ggaattggca ttctttatgt
    10561 caaagaagcc ttgttgcagg agatgccgcc gtgggaaggg ggcggttcta tgatcgccac
    10621 cgtcagcctg agtgaaggca ctacctggac caaagcacca tggcggtttg aagccggtac
    10681 acccaatacc ggaggcatca ttggtcttgg cgcggcgctg gagtatgttt cggcgctggg
    10741 gcttaataac atagccgagt atgaacagaa tctgatgcat tacgcgttat cacagttgga
    10801 atctgtaccg gatctcactc tctatggccc acaaaacagg cttggcgtta ttgcttttaa
    10861 tctcggtaaa caccacgcct atgatgttgg cagttttctc gataattacg gcattgctgt
    10921 gcgtaccgga catcactgcg caatgccatt aatggcctat tacaacgtcc ctgcgatgtg
    10981 tcgggcatcg ctggccatgt ataacaccca tgaagaagtg gatcgtctgg tgaccggcct
    11041 gcaacgtatt caccgtctgc tgggataaca gggaggcact atggctttat tgccggataa
    11101 agaaaaattg ctgcgtaatt ttttacgctg cgccaactgg gaagagaaat atctctacat
    11161 tattgagttg ggtcagcgtc tgccagaatt acgtgacgaa gacaaaagcc cgcaaaatag
    11221 catccagggc tgccagagtc aggtgtggat tgtgatgcgt caaaatgcgc agggaattat
    11281 tgaattacag ggcgacagcg atgcggccat tgtaaaaggg cttattgcgg tcgtctttat
    11341 tctttacgat caaatgacgc cgcaggatat tgtcaatttc gatgtgcgcc cgtggtttga
    11401 aaaaatggcg ctcacccaac atctcacccc atcacgctca caaggtctgg aagcgatgat
    11461 tcgcgcaatt cgcgccaaag ccgctgcact tagctaaact aaagggacag ctttcatccg
    11521 gttggctcac attgccgggt ggtcaatgtt tacactggcc tgcacaggag tgttttggtt
    11581 tcaggtgaac ataagcgcca tccgacgttt cagcgtgagt ctccggcaaa tacaggaggt
    11641 ttacaatgaa atgcgcgtct ttgcttacac tcacgcttat cggcgctttt agcgccatcc
    11701 aggctgcctg ggcggttgat tatccgctac caccaaccgg cagtcgactg gttgggcaaa
    11761 atcaaacgta tacggtgcaa gaaggggata aaaaccttca ggccatcgcc cgacgttttg
    11821 atactgcggc aatgttgatc cttgaagcca ataacactat cgccccggtg ccaaaacccg
    11881 gtacgacgat aactattcct tcacaactat tattacctga tgcgccgcgt caggggatta
    11941 ttgtaaacct tgcagagctg cgcctttata attatccgcc gggagaaaat atcgtgcagg
    12001 tttatccgat aggtattggt ttgcaggggc tggaaacgcc ggtgatggaa acgcgtgtgg
    12061 ggcagaaaat ccctaaccca acctggacgc ctacggcggg cattcgtcaa cgttcgctgg
    12121 aacgtggcat taaattacca ccagtcgttc ctgccgggcc caataaccca ctaggacgtt
    12181 acgcactgcg cctcgcgcat ggtaatggcg aatacctcat tcacggcacc agtgcgccgg
    12241 acagcgtcgg tttgcgcgtc agttcagggt gtattcgcat gaatgcaccg gatattaaag
    12301 ccttattctc cagcgtgcgg acgggaacgc cggtgaaagt gatcaacgaa ccggtgaaat
    12361 attccgtgga gcctaacggg atgcgttatg ttgaagtaca tcgaccacta tcggcagaag
    12421 aacagcagaa cgttcagaca atgccataca cactgccagc aggctttacg caatttaaag
    12481 acaataaggg tgtagatcag aaattagtcg ataaagcgtt gtatcgtcgg gcagggtatc
    12541 cggttgcggt gagcagtgga gcaactcccg cagccagcaa tgcgccttca gtagagtcag
    12601 cgcagaatgg tgaaccagag caagggaata tgttacgcgc gacgcagtag cggtaaatgg
    12661 caggcaaaaa aaatggcgca caatgtgcgc catttttcac ttcacaggta ctattacttg
    12721 cggtatttag tagccatgtt gtccagacgc tggttagcac gagctgcgtc atctttagca
    12781 gcctgaacgt cggaacgcat tgcgttcacg tcgttgctca gctggtcaac tttagcgttc
    12841 agagtctgaa cgtcagaaga cagctgatcg attttagcgt tgctggagca acctgccagc
    12901 agagtagaac ccaggattac cgcgcccagt accagtttag tagctttcat tattaatacc
    12961 ctctagattg agttaatctc catgtagcgt tacaagtatt acacaaagtt ttttatgttg
    13021 agaatatttt tttgatggga aggcacttat ttttgatcgt tcgctcaaag aagcatcgaa
    13081 cgctgggttt tagataaaaa aattgagaga aaacaggatg ct
//
LOCUS       DAZGAP010000157        12999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGAP010000157 DAZGAP010000000
VERSION     DAZGAP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.158"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..305)
                     /locus_tag="ACHLAM_003952"
     CDS             complement(<1..305)
                     /locus_tag="ACHLAM_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855408.1"
                     /translation="MKTNWIEHMKNLLVKKPEVKDFFSKDKFPLNLVDGSYPIFISLK
                     DRGIYAAHSIFICSDDELHAIKEHLSNIFNVNKDTIGNEGVIYDSGIVHHPLFRDAI"
     gene            complement(295..1185)
                     /locus_tag="ACHLAM_003953"
     CDS             complement(295..1185)
                     /locus_tag="ACHLAM_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFO7855409.1"
                     /translation="MVISENTVGTNDIPDSNHDILGPLMPARGFFFIVIDEPGKDGFL
                     VRKIYASPNSPYLTKMNLDPLRLLSETEPERYGLMPKDYSAPTTVAEHVMGNNKSSLI
                     STSSIFPDGSPRFEGKTIFIDVHKAKNAGLKLISTAEIITALEEYKKQYPHLAKRVEK
                     ISGYVDDIDKEVLIEGRKVPAQTIFTPESLKTTQNIVKAGRVVQVFSIFFTAYDLEQA
                     TEKSFETKSIKPIGAEAIRQIGGWGGAVAGAKIGTVIGASLGVTTGPGAVATGIIGGI
                     VFGAAGYFGADWVADYIDEN"
     gene            complement(1179..3668)
                     /locus_tag="ACHLAM_003954"
     CDS             complement(1179..3668)
                     /locus_tag="ACHLAM_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7855410.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGREQLSAPFRYDIEFT
                     STDKTIEPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAP
                     AMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAI
                     ASGEHLQLAAAKNVIANAGNNADVGAVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTQGEYNV
                     KAGYYGRKPKAKLTPELMAFPVISAKFDEQVRVIDDDGKFIANVPYYIEDSIGNIYKG
                     FTDSAGCCERIFTEEADNLNVLLGIKALEKW"
     gene            complement(3680..6325)
                     /gene="tssH"
                     /locus_tag="ACHLAM_003955"
                     /gene_synonym="clpV"
     CDS             complement(3680..6325)
                     /gene="tssH"
                     /locus_tag="ACHLAM_003955"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO7855411.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLHPLLDAQSDECPA
                     RQPQVTEPLTSAQPESATTDVPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAAQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     VQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            complement(6517..7008)
                     /locus_tag="ACHLAM_003956"
     CDS             complement(6517..7008)
                     /locus_tag="ACHLAM_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7855412.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVPTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            complement(7066..8718)
                     /locus_tag="ACHLAM_003957"
     CDS             complement(7066..8718)
                     /locus_tag="ACHLAM_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7855413.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDTAVLTGQLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPLGRAVNRRVEISLVPRSDACQDVK"
     gene            complement(8731..9396)
                     /gene="tssL"
                     /locus_tag="ACHLAM_003958"
     CDS             complement(8731..9396)
                     /gene="tssL"
                     /locus_tag="ACHLAM_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO7855414.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            complement(9393..10730)
                     /gene="tssK"
                     /locus_tag="ACHLAM_003959"
     CDS             complement(9393..10730)
                     /gene="tssK"
                     /locus_tag="ACHLAM_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7855415.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     SNVLFDRDALTLGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLTLNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLQEEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            complement(10750..12288)
                     /gene="tssC"
                     /locus_tag="ACHLAM_003960"
     CDS             complement(10750..12288)
                     /gene="tssC"
                     /locus_tag="ACHLAM_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7855416.1"
                     /translation="MSVQQEYATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            complement(12347..12847)
                     /gene="tssB"
                     /locus_tag="ACHLAM_003961"
     CDS             complement(12347..12847)
                     /gene="tssB"
                     /locus_tag="ACHLAM_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7855417.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
BASE COUNT         2743 a         3682 c         3449 g         3125 t
ORIGIN      
        1 tcaatagcat ctctaaagag ggggtggtgt acaatgccac tatcgtaaat gaccccttca
       61 tttccgatag tatctttatt tacattaaat atattagata aatgttcctt tattgcatga
      121 agctcatcat ccgagcaaat aaatattgag tgcgcagcgt atatccctcg atctttcagt
      181 gaaataaata ttggataaga cccatcaact aaattgagtg ggaatttatc ttttgaaaaa
      241 aaatctttaa cttcaggttt tttaacgagc aaattcttca tgtgctctat ccaattagtt
      301 ttcatctata taatccgcta cccaatctgc accaaaataa ccagctgcac caaaaacaat
      361 cccgccaata atcccggtag ccactgctcc tggcccagta gtaacaccca gggacgcgcc
      421 tatcactgtt ccaattttag caccagctac tgctcctccc catcctccaa tttgccttat
      481 tgcttctgca cctataggtt ttatgctttt tgtttcaaac gatttttcag ttgcctgttc
      541 taaatcatat gcagtgaaaa aaatactaaa aacttgtacg acacgcccgg ccttaacaat
      601 gttttgagtc gtttttagag actctggtgt aaaaattgtt tgtgctggta cttttctacc
      661 ttcgatcaag acctctttat caatatcatc aacatagcct gaaatttttt caacacgctt
      721 agccaaatga gggtattgtt tcttatactc ctcaagagca gtaataatct ctgcggtcga
      781 gattaacttc aacccggcat tttttgcttt gtgaacatca ataaatatgg ttttcccctc
      841 aaatctcgga gagccatcag gaaatataga gcttgtagaa atcaacgagg atttgttgtt
      901 ccccataaca tgttctgcta cagtagtggg tgctgaataa tcttttggca tcaacccata
      961 gcgttcaggc tctgtttctg ataataatct aagaggatcc agattcatct ttgtcaggta
     1021 ggggctattt ggtgaagcat atatttttct gacaagaaag ccatcttttc ccggctcatc
     1081 gataactata aaaaagaaac ctcttgcagg cattaagggt cctaatatat cgtgatttga
     1141 atcaggtata tcatttgttc ctactgtatt ctcagatatc accatttttc cagcgcctta
     1201 ataccaagaa gtacgtttag gttatcagct tcttccgtga aaattctttc acagcacccg
     1261 gcagaatcag taaacccctt atagatattc cctatgctgt cttctatata gtaaggtaca
     1321 ttagcaatga attttccgtc atcatcaatt actctaactt gctcatcaaa tttcgcagat
     1381 attacaggaa acgccatcag ctccggcgtc agcttcgcct ttggtttacg cccgtaatat
     1441 ccggccttaa cgttatattc tccctgcgtt cccgcttcta tcccacaggc atccagccgg
     1501 atgtagctgc caccgccgtt aagggtgatt ttcttcttcg cggtgatctt gatctcatct
     1561 tccgtactgg tgatctctat cgatttctgt gccaacagtt ccatcagatc gttctgtgcc
     1621 tgtaccgaga tcgccccctt gttggcaaac agcttgatgc ccgctttacg cacaaacacg
     1681 cttagcgcct gtcccacgcc gataaacata tttttcaccg cgccgacatc ggcgttgttg
     1741 cctgcgttgg ctatcacatt ctttgccgcc gccagttgca ggtgctcgcc gctggcgatc
     1801 gccacgccct tgggtgcgct tagcaggatc gcagcctggt tcagatcttt tacgctctgt
     1861 tgcaacaacg caatctgcga tgacagatct gccggatcgg cattcgccgt ctgcgcatca
     1921 gtggacagag acttcatctg tgactgtgcg ccattcagta tcgccattgc aggagccatt
     1981 tcgcgcacct gcccctgcgc attatcctgc gcatcggcgc tgataaagat gcctttcttt
     2041 gcccgcacca cgccccacat atcggtacgc agttcaaagc cttcgccgcg ttgctgtttc
     2101 ccggcatcca ccagatgacc gaggtttagc tggctcttgc cgccgtactc ggtactgacc
     2161 ttgatatgtt ctttgccgcg ctcatcgtcg aggcggattt tgttgttcgc cggggttctg
     2221 aggacgttac gcttatagtt ctgaatggtc acatggtcag tgtgtgccga gtcgtgtttt
     2281 acgcctgcaa tataaggtcg gtccgggttg ccttcctcaa aggcgatact gacttccgtt
     2341 cccgccagca gcggcaggtg caggccgtag gtgtctcccg catacgggcg cgactggcgt
     2401 acccacaggc tctcatagcc cggtttccac gtatcgcggt caaagtcgag gttgacccgg
     2461 tagcgcccgt ccttgtcgat atgggcgtag atgtcgttct tcaccgtact ggtgacccgc
     2521 gccgggagcg ttccggccat tacggggcgg gggataagcg ccggacggta gccgtagcgc
     2581 tctgagtacg gtatcgccgt gaacgtcagc tcatagctgc ggtcacgcgc cgcgctggtc
     2641 gtcacgccgg taatcagcac gcccttgcgg aacaccgcag gcgcgtcgtc gccctgaact
     2701 tttatctcca gccccggcat cagcagtgaa ctggtgctct ggcctttgag aatggcctgt
     2761 tcattcaggt agcgttcgtg acggatacgg gcataaaacg cgccgctttc cgctgcctct
     2821 ttatcgcctt gttgcaggaa gttgtcggca tagtggtagg cttcgccgta agtggtgtta
     2881 tcgcccccgg tggcgtcatg ctggcctgtg gtcatttccg ccgtggcttc acggtagttg
     2941 tagtcccgcg tggagacact tttttccacc acgctgtacg cggtgttcag cccccatacg
     3001 gcttccgttt caccatcaaa cagcccagaa ggatggcgaa gcggcagcgt cagcccgcgc
     3061 tcgtagccgc tctggtcgtc gtaaaattcg ataacttcga ttttcaaccg ggcgtcggtg
     3121 gcaaagcgga accagatacc cacttctgac agcaggcgtg aaataaacgt caggtcgtcc
     3181 tcaccgtact gcatcacctg ttcacgggac gggtattcgc ttttgaggtt aaacacgaag
     3241 tcctgaccgc gcatctggtg ccgctcgcgc aggatttttt cgacgatttg cggcaccgtc
     3301 tggttctggt agatggcgtt ctggcggctg cgggtaagca gcgccatacg gggttcaagg
     3361 cgcacttcgt aacgggcttc gtcctgtgac gatgagagat gtttaaagcc ggtaatgacg
     3421 ccgtgcaggg tgcgcagggc agtctgtacc ggcattccct gtaccggcgg tgccgtcagg
     3481 ctgaatgcgc catcctgcat cagcaccgac tccggctcga tggttttatc ggtactggta
     3541 aattcaatgt cgtagcggaa gggtgcgctg agttgttccc ggcccgtaaa cgtcagcacg
     3601 tccaccgggc ttttcaggcc gcgaattttg agcttgtggt gtgagtggtc gaaattctga
     3661 atacccataa aagcctccgt tattgcatct cttccgtagc gaactccagt acaatcccgt
     3721 cttcctcatc ccagccaagc cgtagttggg ctggtttacg gtgcgcggcc tgctgtgcca
     3781 gcaactgctg acttagtacc ggcaggattt gctggttcaa caggctgtcg atattgcgtg
     3841 caccggtgtc cggcagcagg caggcggcgg tcagcgcgtc atacaggctg tcgttgattt
     3901 ccgtttccag cccatagtgc tgatgaagac gacgaaccac ctgcgccagt ttcatttcca
     3961 caatggtgcg catcgcctcc tgtcccagcg ggcggtaaat caccgtctga aaacgcgcca
     4021 tcagtgcagg ctggaagtgc tcgcgcagca ggggatacag cagttcatgc aggtctgcgt
     4081 ccgtggcgtc cggtttttcc tccagcagtt gcatgatgtg gtcgctgccc agattagccg
     4141 tcatcagaat cacggtgttg cggaagtcaa tttcccggcc ttcgccgtcg cgcataaaac
     4201 cccggtcaaa tacctgatag aacaggttca ttacgtcgcg gtgcgccttt tccacttcgt
     4261 cgagcaacac cacgctgtac gggcgtttgc gaacggcttc ggtaagtacg ccgccctgac
     4321 cgtagccaac gtagcccgga ggagacccct taagctggga gacggtatgc ggctcctgat
     4381 attcggagag gttgatggtg ataagagatt tttcaccgcc aaacagggtg tcggccagtg
     4441 tgagcgcagt ttcggtttta ccggtgccgc tggggccggt cagcaggaat accccctgtg
     4501 ggccgttctc cggcgtgagg ccggttttag ccgcccgcaa ccgctgtgcg atggcgcaga
     4561 gcgccgggcg ttgcccgaca acgcggtttt caaggctctg ttccatactg agcaggtcgc
     4621 tctgctcgtc cttgagcagg gaagagagcg ggatgccagt ccagtccgcc accaccgtgg
     4681 cgacggcgcg ggcgtccaca tccagcgcca gcagcggtgc aaagccctga tgcgtccgca
     4741 gttgctgttg cagttcagag ctgtctgttc cttcctgacg caacgtcata atctgctgca
     4801 ccagtgcctt ttcctgctga tactgcgctt ccagatgccc ggcttctgcc tgcagctcat
     4861 cacgcaaatc ggtcagttct gccagccgct ccgggctggt ttcgggataa aacacgctgt
     4921 cctgctcaat cgcctcccgc tcgatatcca gcgccgccag tcgcgcatga agctgcgtca
     4981 gcggctccgg cagggtgtcg aggctcatgc ggactcttgc tccggcggta tccagcaaat
     5041 caaccgcctt gtccggcagc tggcgtccgg tcagatagcg gcgcgacagg cgcacggcgg
     5101 tctgaatggc gctgtccagc atatgcacgc cgtggtgctg tgcataacgg gccttcagtc
     5161 cccgcagcat cagacaggcg gtgtcatcat ccggctcgtc aaccttaacc atctggaagc
     5221 ggcgctccag cgcggcgtcg cgctcaaaat actgtttgta ttcgctccac gtggtcgccg
     5281 cgatggtgcg cagttcgccc cttgccagtg ccggtttcag caggttcgcc gcatccgcgc
     5341 cgccagcctg gttacccgcg ccgataatgg tatgggcttc atcaataaac agcagtaccg
     5401 gctccggcga tttctgtgct gcatcaatca cgtttttcag tcgctgctca aattcacctt
     5461 taacacccgc gccagcctgt aacagaccga ggtccagcgt gcggatatgc accgttttca
     5521 ggctgtcggg cacgttgcct tcggcgatac gcagcgccag cccttccacc agtgcggttt
     5581 tgcccacccc cggttctccc accagaatcg ggttgttttt gcggcgacgg gagaggatat
     5641 cgaccatctg gcgaatttcg gtgtcgcgcc cgaatactgg gtcgattcgc ccgctacggg
     5701 ctttttccgt cacgtcttcg gtaaagcggt taagcaccgc cagcagggcg tcatcctgtt
     5761 tccccgtcag cgtttttgcc ggtacgtccg tcgttgccga ctctggttgc gcggatgtca
     5821 gcggttcggt gacctgtggc tgacgtgccg gacattcatc cgactgcgca tccagcaggg
     5881 gatgcagccg ctcaagctgc acgcggctca gactcagcaa tacccacagg tcattacagg
     5941 cgggcagcat cggtttttct gtcagcgcca tcagcaggtg ctggctgcgg atttgtggat
     6001 cgtcaccttc cagtgacgct atcagccacg cctgtctgat aagcgctgcc agcggttcag
     6061 aaagttgagg acgttcgcgg accgagcggg gtaaggtgtc cagatgcgcc agcagagact
     6121 gccagagcgt gtcgatatcc cattcatagc gacgggcaat caccgtgata tcgccttctc
     6181 cctgctccag cagtttcagc agccagtgtt caatggttat ctccgcatgg gcgcgggtct
     6241 gacacagtga ggcggcagcg tccaacgccc gtgcacagta tgggttaagg cgacgcagca
     6301 gcgcggcggg gtgatttcct gtcatagaag ggtttcctta tcgtgttctg tattcctgcg
     6361 atccgcgttc aggcacgcta ccgcccatag ccgttacctc acacaggccc atcaggtttc
     6421 accagattgt gggtatgctt tgctcagtac cggtgcctgc ggcactggac aaagcacaca
     6481 acaaaaaggc agacagtttc ctgtctgccg cagttatcag gcggaaggac gctcattcca
     6541 cgagtcggaa tgaatgatgt tgccgtcttt gtaagtccag gtgattttct cgtagcggaa
     6601 ctcaatacgt tccaggtggt tatgcttctc tctggaagga tccttgatgt cgtgcatcag
     6661 cggagcgact ctgaccagct tgacgttatc aagcgtgatg ttgaagtact ccacttcctg
     6721 accggcatcg ttgatgcggt aaaacttaaa ttctgccgtt ttcagggtct gaccggtggg
     6781 cacagctttg tagagatacg gactggacgc atcgatttct ttggtaaacg taaagggctt
     6841 gtgagtacgg gtaccggtca gtttgccggt gttgttgtcg gtcgggatgt acacatcgtg
     6901 atccaacgcc accacttcga tgctgccttc gcgcccctga acgtccacgg aacctttgat
     6961 atctgcgccg ccgtcatctt tcagccagag ataagcagga atagccatga gaattactct
     7021 ccttgttagt gtgattgaag caccgccttc ttccggcggt atgttttatt tcacgtcctg
     7081 acaggcgtca gaacgcggaa caagactgat ttcgacgcgc cggttgactg ccctgcccag
     7141 tggcgtatcg ttggtcgccg caggctggct ctcacccagt ccctgtacgg caaaacaggt
     7201 ggccgggatg tcgctggtct gcagcatcca gttacgcacc gcttcggcac gtcgcagcga
     7261 taactgctga ttgctttttt cgtcgccggt ggcgtcggta tatccggcca cgaggatcag
     7321 ccagcccggt tttgcccgga tgttcaccag tgcgtccacc agcacttttg tcgagccgtc
     7381 tttcaggcgg gcctgtccga cgtcaaacag cgacatactg tcaagacgca cggtctgaac
     7441 ctgaagcgaa gccgtcacct ccattttttg ttcaggcgga cgccagtcgc gaatggtgcg
     7501 taataccggc tggcggatgc gttcgccggg gtataacccc agaccgaggc gcagtggttc
     7561 tccttcccgg taatacccat cgagcatggt cgcatcgtct ttcagcaccg acaggtgacg
     7621 ggctttggtg ataaattcct ccaccgggac ggcataaaac cggtgcagat cgtcaccgac
     7681 ctgccgtaat aactggcgat ttgccgtggc tgacaggcac agcgcggcga tacccgccac
     7741 ggtggtgacg cccagcatgg tcacgcaggc ccgtcgcagc ggggtaaagc ccgactgacg
     7801 cggcaaccgc cgtaacaggg catccgggaa tggcagcgca tcgtctgtgc cggtgtccgc
     7861 gatatccggc gtcaggccgg ttctggcggt gatccacaac tgccataggt tgttatccac
     7921 cgcaggcaac gaggggacca gccccacggc catcgccagt ggtggacatt tcgcctccgg
     7981 gccgttcagt tccgccagca cattcagatc cagccattgc atcaggcttt cggctttcag
     8041 cagataagag agtcgctgac tgcgttcctg tgcgccggat tgtgcaatcc actgcgcggg
     8101 catggtggtc tctgcggatg ttgctacctg aacttcgccg ccggtacaga taaaccacgg
     8161 tgaattcgct ctctccggca acggcgagcc ggacagcccg ctccagagaa gccagggtac
     8221 gtttacgccc gcccggcgac gcactatcgc catactgtga gcgaaccgtc gcagttgtcc
     8281 ggtcagaacc gccgtatccc ggtacatgcc gggcatgacg gtatacgcca cggcaagctg
     8341 cgatgcccac gccgggcgaa gggtcagtaa tcgctccacc tgcgccacaa gctgttcttc
     8401 atcgggaaca tgcagataca gcccttctga aacctgccgg accgggctgt cagtgaacag
     8461 atgcgctgac atatcgccac agaccagcac taccggttgt cggtacgttt ccggcggcag
     8521 cgataacaac ccggcatcgc cggtcatctc cgtccacgtc tgcctgcgcc ggtaaatcaa
     8581 caatgcggca accgccagaa cgataactgc tgcaccggcc catctcagta cgccagacgg
     8641 actgacaaac acccaccaca gcaccagcag taacaccatt ccccacagca cgatggcctg
     8701 tttcagcgtg ttcctcactc acggctccct ttaccgcagg ctttgcggca gcaattcatc
     8761 caccagcacg ttcagccagt ggtgcagtcc ccaccagaca ccggccagta gcagcgtcag
     8821 cgttaccagc cagaaaaacg gcgaatgcag caaaggatgc tgacgggggg ataacggcac
     8881 gttcagtacc gccgtttccg gcagcacgcc aaatggcgcg accagcccgt tgagcgtcga
     8941 aataagctgg tcacgctccg gggccaccgg atcctgataa cgcccgcgaa atcccagcag
     9001 caggacacgg tgaaagcagg tcagcactgc ctgcgcgggg gcgggctcct gcaacacctg
     9061 cttcatccgt tcgtacagca gctcgcctgc ctgcagagtg ttgaagaaat gcgactggag
     9121 tgggtttttc atccagatgg cctgaccgtc gtccatgcca ctacggctga gcaccgtttc
     9181 gtccagcagg gcgcactgcg cgtaagttat gtgctccacg ttctcccgac tgaatccggc
     9241 atctttcaga cgttcgcgga ccagttccac ctgccgctga cagtggcgat acagctcgat
     9301 accgtgacgc acggtggttc cctgacgcag ttccaccacc gtcagaaacg tgtcgcgcag
     9361 cagcacgtcg atattgatat ccgtctcttt tttcatgagc gcagcaccgc aaacagttca
     9421 ggtttcaggt cgcccattgt tcccggcgca taaatcatgc agcacccgga ctccagcatc
     9481 gatttagccg cgtcactgtg catatccagc gcaaaatact ggttctccag ccgcagcggc
     9541 agcgccgccg gaaccgaggt gagtggcacc agtggaacac cgttcagcgc cacgttgata
     9601 agcagcgtga cgtcatcagg cgcaccgatt ttgcagacca gcggtagctg gtgcagtacc
     9661 tgatgcgcag gcagtgagga acgaacggac aggtaaaaat cggcttcttc ctgcaggcgc
     9721 ggatcgtgca ggtcggcttt ccagcggttg cccggcaggg acgccagatc cagcgcgatc
     9781 acccgcgacg gcaggctggc ttccagcagc tcgctgagca gggagaacag tggcggaaag
     9841 acggtggacg gcgattcgtg gacatagggc ggaacggcgc tgacgttatg ctcaagggaa
     9901 aacgtcagca gcgcaccggc aagcctcgcc agctcccgcc agaccagttc ggggtggaca
     9961 gccgggtagc gcaggaaatc gctcagcacc ggctcggcgg aattcagggc gttaagcagc
    10021 cagaacagag agacatcggc gacggcgaag tccgccatgc gctggttgct ttcccggcgc
    10081 atcgccatca ggcgctggca cttcgcccgt agctgcaaca gcagcgtgtc caggcgctgt
    10141 accagcccgt catgggcgtt taacgtcagt aacggcggaa tatagctctc atcctgcgtc
    10201 cagttgccct gaacatcccg caccagacgc gccagcggac aggtgatgta atcctgattg
    10261 ttatcatggg caaagcgcag ggacagtgca tggcgctcaa cggccatatc ttcactgtca
    10321 ctgccgtaga tatcctgcac cttttgccat tcctgacgat agcgcagcgg cctttcagcc
    10381 tgctcgccct gaccgagatt accgccgtta ccatgcgcaa gcggcagcgc cagcaaaaca
    10441 atcgcgctgc cgccctccag cgtgcgcagg tcacaggcag gcggcagaat atccgccacg
    10501 tctgaatcga tcagcgtacc atcagcaaaa cgcaggcgta ctgcctgcgt tttcagcttt
    10561 ccgagcgtca gggcatcccg gtcaaagagc acgtttgaga tcccccacgg atggcgaata
    10621 caaagctgcg caatgctgtc attgctgaac gcttcccagc gggcctgctg ctgaaactgc
    10681 tgagaagaca ggaatgtccc ctcagcccac agtggtcgac aaattttcat acatcacacc
    10741 accgcccgat caggcttttg cttttggcat ctgggaaacc agagaaaggt tgacgtccat
    10801 cccttcgatc tggaaatgcg gcacggcaaa gagtctgacg cggaaaaagc ccggattgtc
    10861 ctctatgtcc tcgacgataa ctttcccgtc gcgcagtgga tgcgaagcct gaagttcatc
    10921 gcccggatcg gtcatttccg tcaccagcgt gcggatccag gtgttcagct ccagttccag
    10981 cacgcgtctg tctttggtgg tgccgatatt ctcgcgctga atgattttca ggtagtgagc
    11041 aatgcgggac agcaggaaga tatagggcaa acgggcgttg atacggctgt tggcggtggc
    11101 gtcagcggta tcatacagcg ccggtttctg ggcagagttc gctgagaaga agcaggcgta
    11161 atcgcgattc ttataataag agagcgggat aaagccaagg ttggcgaact caaactcgcg
    11221 ggtttccggg atcatcactt cggacggaat tttgacctga ttgccggtgc cgagatcgta
    11281 aagatggatc ggcagatcgg ctactgcgcc acccgcctgc ggaccacgga tctgcacaca
    11341 ccagccatta ttcacaaaac tcttcaccat attggcggca aaggcaaacg aggcgtttgt
    11401 ccagaggtac ttttcgtgat ccgggccttt gacctcttcc acatagttaa agctgcgtac
    11461 cggcaccgtg tccgggccat agggcagacg gcccagcacg cgcggcatca ctaatcccac
    11521 gtagcggctg tcgtcggtat cgcggaacga cttccatttg atatattccg cgcggtcaaa
    11581 atagttgccg atatctttaa ttgcggcgac ttcttccatc gagtttttaa ggaagaactt
    11641 cggtccgacg gaaccgataa agggcatatg tgatgcagca gagactctgg aaatattacg
    11701 cagcagggca atatcctgcg ggctgttatc gaattcatag ctggatatta atgaggctac
    11761 aggttcgcca cccggcgtat catattcctg aatgtatgta tggcgatata aaccactttg
    11821 aatgatttcc ggagaatcat caaagtcctg ccgcagatgt tctttactga tatccagcag
    11881 ttcaattttg acatttttgc gaaaatcggt gcgctgtacg acgaaccatg tgccgcgcca
    11941 gagcgactcg actttctgga attccggatg gtgcatgact gcatcaagct gtttactgat
    12001 tttctgatcc aaatcgtcga tatgaaaatc cagcaggctc ttgtcgagtt taaccacgtc
    12061 ctcgcctgat ttcgccagac aggaaagcag aacgtggatc gccgccgtca cgcgctcatc
    12121 gggtgaagca tcggccagcg tttcgctgtt ttgccataaa tcgatttcct gaatggaaga
    12181 gaccggggtt aaattaattt tatcaaacag ggactgataa acgccgccgg actcagtggt
    12241 ggtgagtgtt gcagtttcag aggtggcata ttcttgttgt acagacatca gcatttcctt
    12301 tttagggatc ctttcttctc cggtttaacc tgaatacgcg ttcgggttaa acgttctcct
    12361 gcggcgcaat tttcgccagt tcatcacgca gttctgaact gagcgtcggg tctttcagga
    12421 tattttccag ttcacggcga aacgttgcgt tatccagcag attggatttc aggtcacgta
    12481 gtaaattgcg catagcgagt aatacacgaa gttgcggaat attttttgct acctgttcgg
    12541 gttcaaaatc cttcaggctt ttaaattcaa gcgcgatatt ttgttcatta ccgtttccgt
    12601 ttagcgtatc ttctactgtt aaattaaccg caggcgaaaa ctcagccatg acgctgttga
    12661 agttgttttt attgatatca accttgtccc gttcggacag cggacgttgc tcttttccgt
    12721 tactgtaatc gcctacggca agaagcttga gggggagttc aactttcttt ttagcattgc
    12781 ctgtatgcag atcaagcttg atatttacac gagcggcggg aacttcattc tggaaactgt
    12841 cggccatatc cttttcctca tcatccactt tttctgatgg ttattgacat aattatattc
    12901 aggggattat cggatgattt aaataaaaag caatgatgag gaaaatatac aaatgaataa
    12961 tgtatttttc aggggaatag atacagttgt atcatttta
//
LOCUS       DAZGAP010000158        12990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGAP010000158 DAZGAP010000000
VERSION     DAZGAP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.159"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..502
                     /gene="rpiB"
                     /locus_tag="ACHLAM_003962"
                     /gene_synonym="alsI"
     CDS             53..502
                     /gene="rpiB"
                     /locus_tag="ACHLAM_003962"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO7855418.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASHVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            571..900
                     /gene="yjdP"
                     /locus_tag="ACHLAM_003963"
     CDS             571..900
                     /gene="yjdP"
                     /locus_tag="ACHLAM_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO7855419.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(928..1686)
                     /gene="phnP"
                     /locus_tag="ACHLAM_003964"
     CDS             complement(928..1686)
                     /gene="phnP"
                     /locus_tag="ACHLAM_003964"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO7855420.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(1688..2122)
                     /gene="phnO"
                     /locus_tag="ACHLAM_003965"
     CDS             complement(1688..2122)
                     /gene="phnO"
                     /locus_tag="ACHLAM_003965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO7855421.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYQLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            complement(2109..2666)
                     /gene="phnN"
                     /locus_tag="ACHLAM_003966"
     CDS             complement(2109..2666)
                     /gene="phnN"
                     /locus_tag="ACHLAM_003966"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO7855422.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(2666..3802)
                     /gene="phnM"
                     /locus_tag="ACHLAM_003967"
     CDS             complement(2666..3802)
                     /gene="phnM"
                     /locus_tag="ACHLAM_003967"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO7855423.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(3799..4479)
                     /gene="phnL"
                     /locus_tag="ACHLAM_003968"
     CDS             complement(3799..4479)
                     /gene="phnL"
                     /locus_tag="ACHLAM_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO7855424.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            complement(4590..5348)
                     /gene="phnK"
                     /locus_tag="ACHLAM_003969"
     CDS             complement(4590..5348)
                     /gene="phnK"
                     /locus_tag="ACHLAM_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO7855425.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5345..6190)
                     /gene="phnJ"
                     /locus_tag="ACHLAM_003970"
     CDS             complement(5345..6190)
                     /gene="phnJ"
                     /locus_tag="ACHLAM_003970"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO7855426.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6183..7247)
                     /gene="phnI"
                     /locus_tag="ACHLAM_003971"
     CDS             complement(6183..7247)
                     /gene="phnI"
                     /locus_tag="ACHLAM_003971"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO7855427.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVQEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7247..7831)
                     /gene="phnH"
                     /locus_tag="ACHLAM_003972"
     CDS             complement(7247..7831)
                     /gene="phnH"
                     /locus_tag="ACHLAM_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO7855428.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7828..8280)
                     /gene="phnG"
                     /locus_tag="ACHLAM_003973"
     CDS             complement(7828..8280)
                     /gene="phnG"
                     /locus_tag="ACHLAM_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO7855429.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8281..9006)
                     /gene="phnF"
                     /locus_tag="ACHLAM_003974"
     CDS             complement(8281..9006)
                     /gene="phnF"
                     /locus_tag="ACHLAM_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO7855430.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     HFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(9027..9806)
                     /gene="phnE"
                     /locus_tag="ACHLAM_003975"
     CDS             complement(9027..9806)
                     /gene="phnE"
                     /locus_tag="ACHLAM_003975"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO7855431.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9912..10928)
                     /gene="phnD"
                     /locus_tag="ACHLAM_003976"
     CDS             complement(9912..10928)
                     /gene="phnD"
                     /locus_tag="ACHLAM_003976"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7855432.1"
                     /translation="MNAKIIASLTFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10953..11741)
                     /gene="phnC"
                     /locus_tag="ACHLAM_003977"
     CDS             complement(10953..11741)
                     /gene="phnC"
                     /locus_tag="ACHLAM_003977"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO7855433.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(11874..12317)
                     /gene="yjdN"
                     /locus_tag="ACHLAM_003978"
     CDS             complement(11874..12317)
                     /gene="yjdN"
                     /locus_tag="ACHLAM_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO7855434.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            complement(12477..12812)
                     /gene="yjdM"
                     /locus_tag="ACHLAM_003979"
     CDS             complement(12477..12812)
                     /gene="yjdM"
                     /locus_tag="ACHLAM_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO7855435.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            12811..>12990
                     /locus_tag="ACHLAM_003980"
     CDS             12811..>12990
                     /locus_tag="ACHLAM_003980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855436.1"
                     /translation="MWISSKVSRAKAPKNGRLPKKAAIIHKIPEFVGGTADFRRRSFS
                     FERKRKKKIIFLPPFI"
BASE COUNT         2563 a         3637 c         3910 g         2880 t
ORIGIN      
        1 tacatcacaa atgttttttg attgtgaagt tttgcgcggg cggggaagat gaatgaaaaa
       61 gattgcattt ggctgtgatc atgtcggttt cattttaaaa catgaaatag tggcacattt
      121 agttgagcgt ggcgttgaag tgattgataa aggaacctgg tcgtcagagc gtactgatta
      181 tccgcattac gccagtcacg tcgcactggc cgttgctggc ggagaggctg atggcgggat
      241 tttgatttgt ggcactggcg tcggtatttc gatagcggcg aacaagtttg ccggaattcg
      301 cgcggtcgtt tgtagcgaac cttattccgc gcaactttcg cggcagcata acgacaccaa
      361 cgtgctggct tttggttcac gagtggttgg cctcgaactg gcaaaaatga ttgtcgatgc
      421 gtggctgggc gcacagtacg aaggcggtcg tcatcaacaa cgcgtggagg cgattacggc
      481 aatagagcag cggagaaatt gagattcgtc cactacttgc atggatgagt aatgactaat
      541 gtggatagag tttctttttg aggttggcta atgaaacgct ttccgctatt tcttttattc
      601 accctgctca cgctgtccac cgttccggcc caggccgata ttatcgacga caccattggc
      661 aatattcagc aggcgattaa cgacgcttat aaccctgacc gtgggcgaga ctatgaagat
      721 tcgcgcgacg acggctggca gcgtgaagtg agcgacgatc ggcgcagaca atatgatgac
      781 cgccgccgcc agtttgaaga tcgccgcagg cagctggacg atcgccagcg ccagcttgat
      841 caggagcgtc ggcaactgga agatgaagag cggagaatgg aagatgagta tgggcgttga
      901 tataggggcc ggcgcgggta ttttccctca cgccacctca atctccatcc catcaaaccc
      961 cgcctcaaac cccgatggta gcgcgttttc catcagccac gcatcaaact gatggctgat
     1021 atgggtcaga atcacccgcg gcgagcggat aacctgattc agcgcaagca cggtatttaa
     1081 atcacagtga ttacgcggtg catcctcgcg cggcgggtgg ctgcaatcaa tcaccattac
     1141 ctgcggctga ttattgcgta aaaatttcag cgttttttcc ggcaggcctg cggtgtcaga
     1201 tagccacgcc acccggctgt gcgccgtttc cagtaaatag ccgaaagtga gttttgagtg
     1261 gttgagcggt agcggcgtga cctgtaatcc ctgcaaatca aacatcacaa atggctccac
     1321 cgtgtggctg aaatcaagca ggcccgaatg tttgaataga tcgtcgcagc cctgttcatc
     1381 cggcgggccg tagaccggga tctgatcgcc cacgccccag cgcagcggaa acagcccctg
     1441 cacgtgatcc atatggtagt gcgtcagcaa aaactgctgg aacgatccgg gcgaccagcg
     1501 atcggcgaga tcgtgccgcc cggcgtcgat cagggtgatc gcgtcgttaa acttcacgac
     1561 gccgctgcat ggctggcggc gatactgcgg tgagcgccgc gctctgacgc aggccacgca
     1621 ctcacagccc catgcgggaa cgccctgtgc gccgccagtg ccagtaagtg tgagggtcag
     1681 gctcatgtta cagcaccttg gtgaagcgga agtggctctg ctcgtagcct tcgcgcaggt
     1741 aaaatcggtg cgcgtcgtgg cgcttcacgt tggtggaaag ctcagtcatt tcggccccgg
     1801 cctggcgggc ttcttcttct gcccatgcca gtaatttgct gccgacattc agaccgcgcg
     1861 cctgcggcat caccaccagt tcctgaattt cgccgatcca gttgacatga tgcagatgaa
     1921 actgcaaatg caggccgatc atgccgacaa cttcgccatc cagcagcgcc agttggtagc
     1981 gcatgttcgg atcgcgcaga ttggcgttaa accccacgcg aaacgcctgg tggtcaaact
     2041 ccgcctgttt tagctcacaa atcagcgcgt aaactgcgtc ggtgtcgtac tgcgtagccg
     2101 ggcgaagctc acaagcaggc atggtgtttc tccttctgat gaatcagcgt cagcaacttg
     2161 tcgaccgact ggcgcaggct gccgtcatta ttgagcgtaa gacaatcctg aggagtatag
     2221 cgggcagcgc gcgccagccg ggcgttaatt tcactggcat tttcacgacc acggttttcc
     2281 aggcgctggc gcaggatctc tggcgaaacc tgtaaacaaa cgggcagcag cgccgattga
     2341 tagcgcgccc gcgcctgcgg cagatgggcg cgtgagccgt tgaccaccac gtcaaatcca
     2401 gcgtgcagcc agaggtcaat ctcgacgccg acgccataat acagaccgtt agcgtgccag
     2461 cttaaggcca gaaggttctg ccccgcgcgg gtaaaaaact cctgctcgct cagggcgata
     2521 tggttttcac ttccggcgct ggcggcgcgc gtgatgtagc gatgcgctac cagtagctga
     2581 gtttgttccc gctggcggag ttccgtcaac agactgtctt ttccggagcc agaggccccc
     2641 attaaccaaa tcagttttcc cgtcatcaga acaccctttt accctgacgc cagacgtggt
     2701 cgatatgaat gtgattgccc ttgcgatgcg ccagcaccag gtcggcgcgt ttgccctcgc
     2761 caatcacccc gcgatcctga agattaagcg cctgcgccgg atttttagtc accagcttca
     2821 ccgcctgcgg cagcgtaaag cggttgctct cgtcatcggc gacgcgaaat gccgcatcga
     2881 gcaggctggc ggggtagtag tcggaagaga ggatatccag caggccaagc tgcgccagtt
     2941 cactggccgc cacgttgccg gagtgcgaac cgccgcgcac gatattcggt gcgcccatca
     3001 gcacgttcat gccatgcttg cgcgaggctt ctgccgcttc gaacgtggtg ggaaattcgg
     3061 cgattacgct gccaagttgg tgagattcag caacgtgggc gtgcgtggcg tcatcgtggc
     3121 tggcgagcgc aatgtgtcgc gcgcggcaca ttgcggcgat ggtttcgcga tttggctgcg
     3181 accagcgtgc ggcgagcgcc agctgctctt cttcatactg ctgcatctgc gcttcggtga
     3241 gcgagtattt gccctgataa tattcgcgat acttctcgcg gttagcgaac tggcgctggc
     3301 ccggcgagtg atccatcagc gacaccagcg tcaccggttc gcgctgcacc agtttttcaa
     3361 acagcggcag cgtggtgtga tgcggcagtt cgcagcgcag atgcagacgg tgctcggcgc
     3421 ggttgacgcc gcgtttctgc gtctcttcga tggcgttgat catcttctcc agattctcca
     3481 gccgatcgcc gccgtcgcgc acgtcgccaa tcgccaccgc gtccagcacg gtggtgatgc
     3541 cgctcgccac catcagcgcg tcgtggctac tcattgccga gtgggcgggc cagtccactt
     3601 ttggacgcgg ggtgaagaat ttatccagat tatcggtatg cagctcaatc agccccggca
     3661 gcagccagcc accttcgccg tccatcgctt ccggctggcg gctctggctt tcggcaaagg
     3721 cgcgaatttt gccatcctgt acttccagtg aaccgtgcac cacttcgttt tccagcacca
     3781 gtttgacgtt attgataatc atgaagagac tcccattggg tgcaggcggt cggcgacgtc
     3841 attgcgtacg gcttcgtcat ggaagatacc gacgatggcc gcgccacggg ctttggcttc
     3901 gcgaattaat tccaccaccg cggcgctgtt tttagcgtcc agcgaggcgg tgggttcatc
     3961 cagcagcaga atcggatagt cgacgataaa gccacgggcg atgttgacgc gctgctgttc
     4021 gccgccggag aaggtcgatg gtgccaggtg ccacaggcgt tcaggcacgt tcaggcgcgt
     4081 cagtaatcgg gcggctttag cggcgcaggc ttcacgcggt acgccggtat cgagcagagg
     4141 ctgcatcacc acttccagtg ccgaaatacg cgggatgacg cgcagaaact ggctcaccca
     4201 gccgatggtg gttttgcgga tctccaccac tttgcgggct ggcgcggtga ccaggtctac
     4261 ccattcctca ccgtgtttga tctggatttg cccttcgtcg ggcagatagt tggcgtacag
     4321 cgagcgcagc agggttgatt ttccgctgcc ggagtggccg tgaagcacca cgcattcgcc
     4381 tgcgttgacg gtgagcgagg cacgttgtaa gacgggcagg cgcacgccgt tttgctgatg
     4441 caggatgaag gttttactga cgttttgtac gttaatcatt tttcgcctcg tggttcgggt
     4501 gtcggatgcg acgctgacgc gtcttatccg acctacgggg agcgcatttg taggccggat
     4561 aaggcgttta cgccgcatcc ggcaccggct caattctgca aaaccgatga caccagcagt
     4621 tgtgtatacg gatggtgcgg gtcgtcgagc acgcggtcgg ttaacccact ctccaccact
     4681 tgcccctgct tcatcaccag caaacggtcc gccagcaggc gggcgacgcc taaatcgtgg
     4741 gtgacaatca ccaccgccag gttcagctcc accaccaggc cgcgcagcag gtcgagcagg
     4801 cgcgcctgca cagatacatc cagcccgccg gttggctcgt ccataaatac cagcttcgga
     4861 tgcgtcacca ggttgcgggc aatctgcaaa cgctgctgca taccgccgga aaaggtggtc
     4921 ggcaggtcgt cgatccggtt ggcgggaatt tccacctctt ccagccactt ctgcgcggtg
     4981 gcacgaatat cgccgtaatg acgcgccccg gtcgccatca gccgctcgcc gatattgccg
     5041 cctgccgaca cctggcggcg caggccgtcg agtggatgct gatgcaccac gccccattcg
     5101 gtgcgcagca ggcgacggcg gtcggcctcg ctcatgccat agagggaacg gttttcatag
     5161 cgaatttccc cttgctgcgg cgtcaggcgc gcggagatcg acttcagcag cgtggtcttc
     5221 ccggagccgg attccccgac gatgcccagc acttcccccg gccataaatc aaaagagaca
     5281 tcgctaaagc ctttgcccgg cgcgtaaagg tgggtcaggt tattgaccga aagtaacggt
     5341 tgattcattg gcttttcgcc tcgctctgtt ggcggcaata atcggtatcg gagcagacaa
     5401 acatgcggtt tccggcgtca tccagcacca cttcatcaag atagctgtgg gtcgatccgc
     5461 agatggcgca tggctcattc cactgctgaa cggtgaacgg gtgatcgtcg aaatcgaggc
     5521 tttccacgcg ggtaaacggc ggcaccgcat agatgcgctt ctcgcgcccc gcgccgaaca
     5581 gttgcagggc gggcatcatg ttcattttcg ggttatcaaa tttcgggatc ggcgacgggt
     5641 ccattacgta gcgcccgttt accttcaccg gataggcgta ggtggtggcg atatgaccga
     5701 agcgggcgat atcttcatac agcttcacct gcatcacgcc gtactcctcc agcgcgtgca
     5761 tggtgcgggt ttccgtttcg cgcggctcga taaagcgcag cggttcgggg atcggcacct
     5821 ggaaaataat gatctgatct tcggtcagcg gcgtttcggg gatgcggtgg cgcgtctgga
     5881 taagcgtcgc atcgtccgta cgttcagtgg tgtttacccc ggtcacgcgc ttaaagaagt
     5941 tacggatcga caccgcgttg gtggtgtcgt ccgcgccctg gtcaatcacc ttcagcacat
     6001 cgctttcgcc aatcacgctg gcggtaagct gtatgccgcc ggttccccag ccgtacggca
     6061 tcggcatctc gcgcccgcca aacggcacct gataaccggg gatcgccacc gcttttaaga
     6121 tggcgcggcg gatcatgcgt ttggtctgct cgtcgaggta ggcaaagttg tagccgctca
     6181 gattagccat ggttctgctc ctgttgcaga cgtttgagta gctccagttc ggcctggaaa
     6241 tcgacgtagt gcgggagttt aaggtgcgag acaaagcctg cggcttcgac gttgtcggca
     6301 tgcgccagca cgaactcttc atcctgcgcc gggcctgtcg cgtgctcgcc gtactccggg
     6361 gcttgtagag cgcggtcaac cagcgccatc gccatcgctt tgcgctcgct catgccgaac
     6421 accagcccgt agccgcgcgt gaagtgcggc ggctcatccg gcgggtcgat aaaaccgttg
     6481 accatttcac attcggtcat cagcagttcg ccgacgttca ccgcaaatcc cagctcttcc
     6541 tgcacaatcg acacgtcgat atagccgctg cggatttcgc ctgcgaacgg atgattgcgt
     6601 ccgtaaccac gctgggtgga gtaggccagc gccagcaaat agccttcgtc gccgcgcatt
     6661 aattgttgca ggcgggagga gcgtgagcag gggtaaaccg gcggcgtgcg ggtgatgtcg
     6721 tccggctgtg cgccgctatc ttcttcaaac ttcgccagcc cctgacgcgc cagcaggctg
     6781 aaaacgtgcg gcgacggctg ttgttcgctg tcggctgtgg tcagcgtcgg cgcttcgccg
     6841 tttgccagca gggtgaaatc gagcaggcga tgggtgtagt cgtaggttgg gccaagcagc
     6901 tggccgccgg gaatatcttt ataaaccgct gaaatacgcc gttcgagacg catctccgtg
     6961 gtgtcgagcg gctcgcttac tgcgagcttc gccagcgtgg tgcggtaggc gcgtagcagg
     7021 aaaatcgctt cgacgttatc gccgctggcc tgtttcagcg ccagcgccgc cagttcgcgg
     7081 tcggcaatgc cgccttcggt catcacgcga tctactgcaa gattaagctg ctgttcaatc
     7141 tgggcgacgc tcagttcagg caaatcggta ttgccccggc gtcggctctc ttgcagggcg
     7201 tgggcggcgt cgatcgcctt ctcgccccct ttcacggcaa cgtacatcag catacctcca
     7261 catgagtggt tcgcgggata gccagcaggc gctcgccgca ggtcaggatc aggtcgatgc
     7321 cgagcgggaa tgggtgcggg cgctcggtaa gttcgtgcag aatgcactcc ggcagctgcg
     7381 gggcgatcat tcgttcttcg gcaatacccg cgccagtaag gcgcagcatg cgcccgccgc
     7441 tcaggctgac gacctgtaaa atcagcgtcg cgcccgcttc tggcgcaaca gcggtgccgc
     7501 tggaaagggc gttaagctgt tcgctggaaa tcgcctcatc cgttaccgcg aaggtcgcca
     7561 gttccggctg attgaccagc ggcgcgttgg tatgaaaacg caggctctgg ctgacgatat
     7621 cgttacttaa tggcgcagaa agccacaccg gcgtgtcgtt atcggccagc gtcagcagca
     7681 cgctggtggt ggcgatattc agcggctgcc agccgcgttt gagctgatgc agggcgacaa
     7741 tcacgcccgg ctcgctcatg gcttttaaca ggcgacgaaa actgtgctgg gcatcctgca
     7801 cgggaagcat aaaagcggtt tccagggtca tgcgttatct ccgcgaacca tcgtaaagaa
     7861 gtcgacccgg ctggcgttta cttcggcctg gcgtgcggca atgcgtgcca tacggtcagc
     7921 gttcagcggg gcaataagag tttctgataa gttttgaaaa tggcgggttt gctgcatcag
     7981 cgcgtcaatc agcgcgcagc gttcggcgtg ctgtttgtcg cgccccagca cccagctgta
     8041 gccgagcgtg ccgtcagtca ggcgtacggc ggcgcgggtg agcgtggcgt cgccagcaaa
     8101 aaagcgttcg ccggtaccac ccatccgcgc ctgaatctgc accaggccgg tttcagcggc
     8161 gcggattact tcatagtcgg cggtgatgtt cagcgcgttc aggcgggctg ccagctcagt
     8221 cggttggctg tgcgccagta cggacatcca gtgctggcgg gtcgcggtat ctgcgtgcat
     8281 tcagtgctcc atagtgaatt caatcatgtc ggcgcgcgtc aggctgacgg agtactccgc
     8341 cgggctgctt tcaccgtcac ggtggttaag ggtgcgcacg cacagcagcg gcgatagatt
     8401 cgggatttca agacgctggc actctttggc ctgggcgcgg cgggcgctga tccgcgtctg
     8461 actgcggcgt agcgtaatcc ccgtttgctc gcgcagaaaa tcgtgcagcg aaccgctgtc
     8521 gaaatgttgc agcgtcggcc agagggtgag gtccgcaaag tagtggtcga ttaaacagag
     8581 cgcgacgccg ttgacccggc gcagggtgcg caggtggatg acgttctccc cctcggttat
     8641 ccccagcgcg tcagcgacgt gaccggaggc ggggcgcagc accgatagca atttttcgct
     8701 ggtgggatgg ctgccttggt ccagcaggtt ctggctaaaa cgcgcctggg cgttgagcgg
     8761 gtaatcgaac gggcgcatca gcaccagcac gccgacgccc tgacggcgct gtacccaacc
     8821 tttttccacc agttggtcga tggcgcggcg cagggtgtgg cggttcacct caaagcgcgc
     8881 tgccagttgc tgctcggcgg gaagatagtc gccgcagcgg tagtgttgac gaagctcctg
     8941 ctcaagtttt gcggctatct cctgatagcg tgttgggtag ctggtcggat gtgtagacaa
     9001 gtgcatagct atcaatgcct cgcttatcag ataaagtgct tacgcaaccg ttgagagagg
     9061 aaatccagca ggctgaccgt gacgatgatg agcaccatca gggcgcaggt ttgctggaac
     9121 tggaaaccgc gaatcgcttc ccacagggtg acgccgatcc cgcctgcacc gaccatgccg
     9181 acgacggtcg ccgagcggac gttggattcg aagcgataga gggagtagga gatcagcagc
     9241 ggcatcacct gtggcagcac gccgtagagg atctcttcca gcttgttggc accggtggcg
     9301 cgaatgccct ccaccgggcc gggttcgatc gcttctaccg cttcggaaag cagcttggag
     9361 agcacgccgg tggtgtggat aaacagcgcc agcacgccag cgaacgggcc aaggccgacg
     9421 gcgaccacga acagcatggc gaagaccatt tcgttaatgg cgcggcaggc gtccatcagg
     9481 cggcgtacgg gctggtaaac ccaccacggc accaggtttt cggcgctcat cagaccgaag
     9541 gggatggaga gaaccaccgc cagcgcggtg ccccagacgg cgatttgcag cgtgacggcc
     9601 atttcggtga ggtaatcctg ccactggctg aaatcgggcg ggaagaagtc ggcggcgaag
     9661 gttgccatgt tgccgccgtc tttgatcagc gtaagcgggg ccatttccgc gccctgccac
     9721 gagacgacca acacggcgag aacaatggcc cagctcagaa gcgagaacca gctgcgcttg
     9781 ggtggggcga tggtgatggt ttgcatgttt ggctccggtc tgtaggccgg ataaggtgct
     9841 tgcaccgcat ccggcatcaa cgtctgcaca ttgtcggatg cgacgccggg gcgtcttatc
     9901 cgacctacga tttactgcac cgctttactc accgaactca tcgcgcttaa cgcgttgttc
     9961 aggcggtcca ggtcatccag ttgcgcctga atggcgctgg tttttgccag cttgtcctgc
    10021 tcattcagcc ctttattgcc tttcacgccc tgcatctctt taaacagcgc gagctggcga
    10081 atcggcacca gttgcaggtc gctggaggcg cggaacgggg cccagcccag gcgttccagc
    10141 accgctttct cttccggcgt tttgccgtag ttcataaaga agtcgtagat cttgtctttg
    10201 gtggtttcgg acagattttt gcgccagacg atcggatcgc ctgggatcag cggcgatttc
    10261 cagatcacct tcagttcttt gagcttctcc ggtgcggagg ttttcagctt gtcgaggttt
    10321 tcggtgttgt tggtggcaac atccacctgc ttgttggcaa cggccagcgc gttggtttca
    10381 tgcccggcgt tgacggtgcg cttgaagtcg ctggcggaga tattgttttt ggcgaagacg
    10441 tagtagccgg ggacgaggaa gcctgaggtg gagttaggat cgccattgcc gaaggtgaga
    10501 tctttacgct tggccagcag atcgttcagg ttgttgatcg gactatcttt gttgacgatc
    10561 aacacgctcc agtaacccgg cgatccatcc gccgcgaccg tctgggcgaa gacctggcca
    10621 ttggcgcgat ccaccgcttc catcgctgac agattgccgt accaggcgat atccactttg
    10681 ttgaagcgca ttccctggat aatgcccgcg tagtccgggg caaagaaggc gttgaccttc
    10741 acgcccagct tcttctccat atcctgtaag aacggcgtcc attgcggttt caggttttgc
    10801 tgtgattccg ttgaaataat gccgaaattc agcgcctttt cctgctcttc ggcgtgcgcc
    10861 gggcttaaca gggtgctgag gctgaacatg ctggtgaagg tcagcgaggc aattatctta
    10921 gcgttcattc gttttcctca atgatgggga tgtcaggcag ctttcgcgtt ttcttcgacg
    10981 cggttgatgc tgcggtagag atggtcaaaa cgttcgttat caaactgttg gctgctgccg
    11041 tcgtagaaga cgtgcccctg gcgcagggcg acgatgcgtt cgcagtagcg cagggcgtaa
    11101 tccacctgat gcagcgtgac gaccacggta atgccgtcgt tctggttaat gtcgcgcagg
    11161 gtgtccatca cgatgcgcgc cgattccggg tccagcgagg cgatgggttc gtccgccaga
    11221 atcaccttcg cctgctgcat cagcgcgcgg gcaatcgcca cacgctgttg ctgtccgccg
    11281 gagagggtgg aaacgcgctg atgggcaaag tgcaccatgc caacgcgggt cagcgcctgt
    11341 aacgcgcgct gtttctgctc gcgggtgaag tagctaaaac aggtgcgcca gaatggcgtg
    11401 ctgccgagcg cgccaatcag cacgttctcc agtacgctca ggcgattcac caggttgaat
    11461 tgttggaaga tgtagccggt attggcgcgg cttttgcgga tatcgcgggc caggcggcct
    11521 tcgcgctgga ctgtgcggcc cagcagctcg atatggctac cggcagattt atcgccggta
    11581 atcaaaccgc ttaagtgacg taaaagggtg gatttgccgg aacccgacgg cccaagcaga
    11641 gccaccattt caccgtgatg aatgttcaga tcaaccgcgt gcagcgcctg atgctgattg
    11701 aaggttttgg cgagcttctc gacacggata atcgtttgca tgatgcagcc tccctaaaaa
    11761 agtggctcca tcgtggcgga ttattgtgac gattttgtta actaaaagtg actaacggat
    11821 gaagagttaa cgagatttcg atgacagtgc gggagggcgg ccctcccggc gggttagtgc
    11881 gttggttgtt gtttgacgac attaatcatc cacggtacgc caaatttatc ggtgactttg
    11941 ccgaagccat gcgcccagaa agtttcctgc caggccattt cgatttttcc gttagctgca
    12001 aggttatcaa accagcgttt tccttcttcg acctgttgcg aatcgagcac tagcgtaaag
    12061 ccggagtagc tggcttttcc tgacggtata gcatcgctca tcatgatgtc gcttcctgca
    12121 atgcgcacgt tggcatgagc gatggcggta tcgggaaatt gcattccgga agggcagttc
    12181 tcggcgctgt cctgcgctga tttcggcatt tcgccgaagc tgattttata gagcagttcc
    12241 gcgcccaacg tacgttgata ataggcaatc gcgtcggaac agttaccggc aaaagagagg
    12301 tagggactta acggcatgat ggtgacctca gttaagagaa gcccgttaag tgtagttcga
    12361 aatttataca gatgagaggg aagcctgata agacgcggtc agcgtcgcat caggcagtcg
    12421 ccaccgttgc cggatgcggc gtaaacgcct tatccggcct accgatcaca atacaatcag
    12481 ttctttttca caaactcaga cttcagtttc atcggaccaa aaccgtcgat tttgcaatcg
    12541 atgttatggt cgccttcaac caggcggatg tttttcactt tggtgccaat tttcagcatc
    12601 gaagagctac ctttcacttt cagatcttta atgatggtaa cgctgtcgcc gtcagccagc
    12661 agattgccgt tagcatcttt aacgatcagc tcgtcgcttt cctgtgcagg ttctgcgtcg
    12721 ttccattcgt aggcacattc cgggcagatg tacatgccgt tatcttcgta ggtgtattcg
    12781 gagttgcatt ttgggcagtg tggtaatgac atgtggattt cctcaaaagt cagcagggca
    12841 aaagcgccaa aaaacggcag attgccgaaa aaggccgcaa ttatacacaa aatccctgag
    12901 tttgtcgggg gtactgctga ttttagacga cgatcgtttt catttgaaag gaaacgcaaa
    12961 aagaaaataa tctttttgcc tccatttatt
//
LOCUS       DAZGAP010000159        12762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGAP010000159 DAZGAP010000000
VERSION     DAZGAP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.160"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..866
                     /locus_tag="ACHLAM_003981"
     CDS             <1..866
                     /locus_tag="ACHLAM_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO7855437.1"
                     /translation="TTATPTTAVSSQSTALPPRPLLQATQLPALVMTGKPGQSSVVYL
                     REARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHRALNTLLTGQQL
                     YGHMDWNRKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTAADQAQVPPAVS
                     ASRAQSTTGTSGTSAPRTTTPAPVVTKPVAPPPQKVTPPRQWKMEKGTTLKDGLARWA
                     SSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEKPLYARTSTAQ
                     CLLRVTDRPQE"
     gene            871..1308
                     /gene="pilM"
                     /locus_tag="ACHLAM_003982"
     CDS             871..1308
                     /gene="pilM"
                     /locus_tag="ACHLAM_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO7855438.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSITLPSVIPDHVILWMN"
     gene            1339..2958
                     /locus_tag="ACHLAM_003983"
     CDS             1339..2958
                     /locus_tag="ACHLAM_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO7855439.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLQGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2979..4274
                     /gene="pilO2"
                     /locus_tag="ACHLAM_003984"
     CDS             2979..4274
                     /gene="pilO2"
                     /locus_tag="ACHLAM_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO7855440.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPLYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAAFSA
                     GAQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSLTDENGQIIQPPWKEYDLQMQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            4264..4716
                     /gene="pilP"
                     /locus_tag="ACHLAM_003985"
     CDS             4264..4716
                     /gene="pilP"
                     /locus_tag="ACHLAM_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO7855441.1"
                     /translation="MPDKAKTLLLSALLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEGGRQQESISAVTTNAQALPSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            4801..6309
                     /locus_tag="ACHLAM_003986"
     CDS             4801..6309
                     /locus_tag="ACHLAM_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO7855442.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNTGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            6311..7411
                     /locus_tag="ACHLAM_003987"
     CDS             6311..7411
                     /locus_tag="ACHLAM_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO7855443.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            7485..8021
                     /locus_tag="ACHLAM_003988"
     CDS             7485..8021
                     /locus_tag="ACHLAM_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO7855444.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            8075..8548
                     /locus_tag="ACHLAM_003989"
     CDS             8075..8548
                     /locus_tag="ACHLAM_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO7855445.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            8567..9202
                     /locus_tag="ACHLAM_003990"
     CDS             8567..9202
                     /locus_tag="ACHLAM_003990"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO7855446.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            9207..10571
                     /gene="pilV"
                     /locus_tag="ACHLAM_003991"
     CDS             9207..10571
                     /gene="pilV"
                     /locus_tag="ACHLAM_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO7855447.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCSPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            11186..12352
                     /locus_tag="ACHLAM_003992"
     CDS             11186..12352
                     /locus_tag="ACHLAM_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7855448.1"
                     /translation="MFQKKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHPM
                     ADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGTC
                     RMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEIL
                     SLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFKS
                     AWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLRA
                     YHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISHA
                     SVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            12394..12684
                     /locus_tag="ACHLAM_003993"
     CDS             12394..12684
                     /locus_tag="ACHLAM_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855449.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT         3296 a         3519 c         3108 g         2839 t
ORIGIN      
        1 caacaacagc gacgcccaca accgcagtca gcagccagag taccgcactg cctccccgcc
       61 cgctccttca ggccacacag ctgcccgcac tggttatgac cggtaaaccc ggtcagtcat
      121 ccgtcgttta tttacgtgag gcacgtcagc gctccgtcag tgactggata cgccagttgc
      181 tgccacccgg ctggcagttc cggcaacaga acgctgccat accggctctg aacaaacgcc
      241 tccccgtctg gtccgttaat gaccagtggc accgggcact gaatactctg ctgaccgggc
      301 agcaactgta cggacatatg gactggaaca ggaaaatact gactgtcacg accactgcag
      361 caccgcctgt tgacctcacg gcaccgcagg gttcacagaa aacagcggac acaccacgta
      421 atccgttcag gagcacaacc ggaacggcag cagaccaggc acaggtacca cctgccgtca
      481 gcgcttcccg ggctcagagc acaacaggga catccggtac atctgcgccc cgcacaacca
      541 cgccagcccc ggtggtcacg aaaccggtag cgccaccacc acagaaggtc acaccgccac
      601 ggcagtggaa aatggaaaaa gggaccacgc tgaaggacgg actggcccgc tgggcgtcgt
      661 ccgaaaaatg cccccacggc acctggacgc tacgctggga aacgcccgtt aactacagta
      721 tcgatgcccc tctggttttc accggcagtt tccgccaggc gctggaacag ctcttcgcac
      781 tctacacccc ggtggaaaaa ccgctgtatg cccggacaag cacagcacaa tgtctgctgc
      841 gggtgacaga tcgtccacag gaataacctt atgggctggt tcatcgtgtt catcatggtc
      901 atcacgacca caggcaattt ctggttcacc agtcagctct cccgcagcga acaccgtcat
      961 caggccgcag aaaacacaca acaggcagca acgttcattc gttatatgaa cgccatcaat
     1021 gattacctgc atcagcatcc ggaacgacgg acagccggag ggcggctgac atccgctcag
     1081 ctcgggattc ccgggacgga taccgtcagt catatcatca gccagcaacg tgtttttgtc
     1141 tgggccacag aaaccccggg cctgatggcc gcgctcaggg aacagagtaa tgactctgct
     1201 ctgctcggca gggtggaaaa cggccgactg ctggacactg ccggcagagc gctttccatc
     1261 acacttccgt cagtgatccc cgatcacgtc atcctctgga tgaattagtc acgaatctga
     1321 ataaatcacg gatctcccat gaataaaaaa attcttgcca ttatgattgc cgccgctctg
     1381 ttacagggct gtgccagtta cagacagctt gagagcacaa gaaaggaagc cgttgcggaa
     1441 caacaacggg cacgggaaat gatgtctgat ctgcgaaccc ggacgccggt tgttaaggaa
     1501 tcctcacgac aatggatcaa tccccgtccg gtcatggata aaaacaccgg caaaaccgtt
     1561 cccggttgtc ccattgtcat tcatacccgt cagagcatca cccttcagca ggtggttcag
     1621 cggatcacgg aaagctgcca tattccggta cggatcacac ctgatgtact gacatacctg
     1681 aatacatcca caaccggcag cacacaacaa atccagggcg cgttaccccc accggatgcg
     1741 agcggcatgg ttccccttgc cgccattggc gcatctgaca cgccaccatc ccgtctgcag
     1801 ggaaataccg cagcactgtc tgaactgaat gcctccggcc tgacgaccct gctgaatgcg
     1861 gtctcctccc gtctcggtat atcctggcgc tatgacggct cgcatatcat cttctattat
     1921 ctcgagacac agaccttccc catcacctat atggacagtc aggtggccta taacgccact
     1981 gtggtcagcg gcaccatgtc ctcaaacggc tcatcaggag gcagtgcatc agatgccatt
     2041 tccggtgatg ccagcaatac ccagaccacc accgtggata tgaagtctgc gctgtatcag
     2101 gatgtgaaaa atgcggtcac atcaatgctg accccgggta tcggacgcat gttcatgtcc
     2161 acgggcttta tcaccgtcac agataatcca caggttctgg aaacggtccg ggcattcatt
     2221 gaaaaacgca atgaagagat gaaacgtcag gtggtgctga atgtggaaat tctgagtatc
     2281 cggaaaaccc gcaatgagca ggccggtatc gactggaacg cagtcttcag cgacagaacg
     2341 ctgggactga gtctcggcag cacattcacc agtgcagcca gtgatgccat cacaggtggt
     2401 gtttccattg tggacggtaa actcaccggt tcaaaagcct tcctgaaagc gttgtcttcc
     2461 cagggggatg tcagtgtggt gactcagaac tctgccgtca ccaaaaacct gacgccggtg
     2521 ccaatgcaga tcgccaatca gcagagttac atcgagagtg tgacgacgga taccactgcc
     2581 aatgtcggct catccacctc cctgaatgcg gccaccatca ccaccggttt taacatgacc
     2641 cttctgccgt tcattctccc ggactcacag acactgcagt tgctctattc catgagtctc
     2701 tccgacaaac cggttattga gaattatgag agcggtggaa gtaaagccca gctgccgaat
     2761 gtggatctga aaaccatcaa tcagaccgtg gatctgaaat ccgggcagac ggtcattatc
     2821 agtggcttcc agcagtcagg acgtcgcagc ggaaaacagg gtgtaggcac gccgggattc
     2881 ttcggtcttg gcggtgggat caacagtgaa aacgacgaca ccattctcgt ggtgctgatc
     2941 acgcccaata tcatctgatt tgcacaagga ggacgatcgt gacggatatt tcaccgacag
     3001 ggcggcaggt gtttccgctg acggcagggc gcaatgtgtt tctggccggt ctggaatgga
     3061 aaacactgcc ctcacagtac cggcatgccc gcgactttgc ccgggcacag aaagccgacc
     3121 ttttcctggc ctgtcagtat ctcagtaatg aagatgccga tacccacacc atggtagcca
     3181 ctgtcagtcg ccgcatactg cccggaaaac cccgtcagtg cttctccctc gccctgctga
     3241 tcctgccact acttgaacat ggcggatatg ccatcacgga actgacgtta cccggagaaa
     3301 caccgctcta tagctttgtt tccgttgtcg acggtgttct cgtcagtgac ctggtgggaa
     3361 gcggggaaga ggttcgggaa gcccgggata cctttctttc catcaacacc gaaccgactc
     3421 agggctggat acactatgaa ccagcggcat tttcagcagg cgctcagaat cagcacctgc
     3481 cgctggcctc cctgactggc tctggtaaac atccggctgc cgcacgcctg aatccggtat
     3541 cccggggacc gcagttcatc actgtctctc tgatcatcgc ccttctcggc actgcatggt
     3601 acggctggca gtattatgaa cgctggcaga cagaaaaagc cgcgcaggca gcggttgccc
     3661 gacaggccac agaggaacga atcactcccc cctggcccgg actccctgaa acagacgcgt
     3721 tcatccgggg atgtgctgat atatggactt cactgccact gtctgctgcc ggatggcgtt
     3781 attcgctggc agaatgctcg actgacggtg gcagcggcaa tcttcgcgca tcgtacacca
     3841 atactgcggg cgcgacagtg actgacttca tccggcgcat cagtgatatg acagacagcc
     3901 gaccgtttat tgtgatgccg gaaggaaaga ccggcggcat tggcctgccg gtcaccttcc
     3961 ggctgtctga aaacgccgtc acagttgatg cactaccgga aaccgctgtc ctgcaggaac
     4021 gactgacctc gctggctcag tcaatgcaac tgaaactgga ctggcaggaa gttaataaca
     4081 gcctcactga tgagaacgga cagatcattc agcccccatg gaaagagtat gaccttcaga
     4141 tgcagaccac actccctgca caccatctgg ctgaacgcct gaaagagcct tctgtccgtt
     4201 ttatctctgt cacacggcaa ctggagaacg gtcgtttcca ctatcaattt acaggtaaat
     4261 attatgcccg ataaagccaa aacccttctg ctttcagccc tgctgttacc ggcaacggca
     4321 atggccacaa ccggaaatgc agagcaacag aacacacagc aaataaggat tgaacagata
     4381 caggaagaga cagccctgta tgaagccatg ctggcgagag agaagatccg ccatgaactg
     4441 cgaacacttg agcagggcgt tcccgcagag ggcggacgtc agcaggaaag catttccgca
     4501 gttacaacaa atgcacaggc attaccatcc ccccgcctga cagcaatatt cggtgttcag
     4561 cctcaactga ctgccaggat agaacttccg gggggaagta tctccgatgt gaaggcgggc
     4621 cagaacatcc ccggaaccac ttatcacgtg gaaaaaatca cgccggacag tgtagtgctg
     4681 gaacagtctg gcaccagaaa aaccctgcga ccatgagact ggccgccgta actggcggct
     4741 gtcaaaaata aaaggatgaa agacatattt tccgggcacc gctgcccgta aggaggcttt
     4801 atgctggatc cagcagatct gccgccggat gatattctgg attatgtggc catcgacacc
     4861 gacaacaccg gacaactgcg gatcagagtt gtggagggga aaaaacatct gcgtgccgtc
     4921 caggaatatc tgacccgcct tcgcgcacgc tatcaggggc gtgtgggaga ctttgagttc
     4981 accacgctgg atgtgatagc ccgcctcaga caggacgcca caacgacagg cgatgaaaac
     5041 gctattaacc cgatacagca gaaaatgctc ggctatatcc gccattctgc ctcccttggt
     5101 gcctccgacc ttcatatgac gccagggcgg gacaacacag acttcaccta tgtggaagcc
     5161 cgggttcatg gcgaactgga agtgctcgat atcctccgga aagaagaagg tctggaatta
     5221 ctgggggcta cctattccgg catgacggat gtcatcaagg gcacacaatt tgatcccggg
     5281 gttccacagg acgcacgtct ctctgaacag tacctgaaac tggctggcct gtttggcgca
     5341 cgctactccc actacccctg cgtgggaggc ctctatgctg ttctccgtct gatcaaggat
     5401 gacagccagc aaatcccgac cttcagtatg ctgggttatc acccggatca ggagtgcacc
     5461 ctgcgccgta tcctgcaacg ccctgaaggc attatcaccc tctctggccc caccggctcg
     5521 ggaaaatcca ccacgctgag aaccgcctct gcggcatacc ttgaacaata cggcttcaac
     5581 aacaccggcg gtattcttct gccacggcgc aggttgttca cgattgaatc cccgccggaa
     5641 ggccgtatcc ccggagcaat tcagacagcc gttatggata ccacccgggg ctgggtggac
     5701 tccatcaaat cagcactgcg tcttgatcct gacagcatcc tgaacggtga aatccgcgat
     5761 cacgattccg ccattacggc aataaaagcc gccatgaccg ggcatctgat gctgaccacc
     5821 atccatgcga acgacccgat taacatcctc gaacgccttg agatggaggg tgttcaggcc
     5881 aggatgattg cagacccgca gttgttcatc ggactgctca gtcagcgtct ggttcagctg
     5941 atatgtcccc actgtcgtca tccatggaat gacgtagcat cccgtcgtac tgaggatgag
     6001 cgacatctgg tggaaacatt ctgtaatgct gatgcggtat atctgagaaa ccatgaaggc
     6061 tgtccacact gctggcgtgg tgtcacaggg cgtacagtga ttgcagaagt catctctcct
     6121 gacgccagat tttttcagtt ataccgggaa aaaggccgta tcgaagctaa aacctactgg
     6181 cacagggaac tgggcgggat gacgcgtaat cagcatctgc tgggaaaaat caacagcgga
     6241 ctggttgacc ctctggcggc ccattacatc tctcccattg atgaggacag ctatacgctg
     6301 ctgcgctgat atgaaatttc tggaaaaact caacagtact gaactggatc tgtctgccct
     6361 gaacagctgg acagccagaa aaaccttcgg tcttaagcgt gccgagttct atgagtccct
     6421 cggcggaatg atacgtgacg gaacgccgtc actgcgcgcc ctggagttta tctgtaatgt
     6481 cgaaactgac tttggcaccc ggaaaggaac atccggcatg tactttctgg ccagagaatg
     6541 tattgcctcc ctgaaagcca gcggtatgct gtcgggggcc ctgcccacat gggttccccc
     6601 ggaagaagcg gcactgatca ggaacggtga agaacgcggg aacatcgccg aagccctgtt
     6661 ccaggtcgca aaaaccgtga aagcacggcg ggaaatggtg agcagtctca tcacactctg
     6721 tctttatccc cttctgctgc tttcactctg tgtggtgaat atgtacaacg cacatacccg
     6781 catcatcccc attgtcagcg cttttgcacc ggaggagaaa tggtcattcc agatgaaagt
     6841 ccttgcagcc atgtcagagc ttgtcatcgg ctatggtctg tggctcagtg gtgccatcat
     6901 cacgcttgtc atcatcatcc gcttctcact cagtcgctat accggcccgg gacgcaggct
     6961 tcttgacaaa ctgccaccat ggtcgctcta tcagactttt catggtgtga attatctgtt
     7021 cagcatatcg tcaatgctgc agataaatat tccgctttca caggcgctgc tcagaattga
     7081 aaaaagtgca gagcataata aatggttaaa ggagagaatt caggcaataa gaaaaaatgt
     7141 attatccggc cagaatctgg ccaccgccat gcgcaattct gactatgatt ttccttcaaa
     7201 aatatgcatc aataaacttc tcttaaattc tgaaagggaa gacagcgtat ctgcaatgaa
     7261 tacctatgcc ggacagtggc tggaagatgc aaaaaagagc gtcagaaaaa gtggattaat
     7321 tatcaccggt atttcaggag gttttgtatt cttttttatt cttaacatga ttagcgcgat
     7381 atacagtatc agcgatatgc tcaagcaata atacctgccg taacactaaa acgcccttca
     7441 atatactttc attaacgtaa caaccattaa tacaggagtc atatatgaac ttcagagcac
     7501 gtctgtcttc ttatttttct ttaaaaaaag ataaagaaga tgataaaggt ttaacattat
     7561 tagaaattgc cggcgcaatg attatcatcg caatagttgt cgcgctcgcg atgagccaga
     7621 tgtccagcac aatggcctcc agtgacaacc agcgcgaact gaaaaacctg cagacaattg
     7681 caaccaaaat gaagcaatac aagttccagg gaagatatac cggaacggat tatatcaaaa
     7741 ccctgacgga aagcggtgca ttgcctgctg atatgattgc aggaggcaac aaagccaaaa
     7801 acgcatgggg tggtgatgtc acgattgctg ccacagcaga caaatatggc tacattatca
     7861 ccagtaataa tgttcctaaa acaaactgtg ttgagctgat taacagcctg cgcagttcgt
     7921 ccatatttac caaaattatg aacacagccc cgaacacggt ggatcccgtt accgtctgca
     7981 gtagcgataa aaacaatatc aagctggaaa ccaacagcta atgccctgcc acacagcact
     8041 ccctccgggg tgctgtttcc cggagcacat aatcatgaaa tggttttatc tctcatctct
     8101 tttcttattc tgtctcccgg caactgccgc cgtaaacagt aacacactgg atagctgctt
     8161 tattcaggca ggaaaacgtt actccatcga tcccggacta ctgaaagtaa ttgcccgtaa
     8221 agaaagcagc ctgaatcccc gggccatcaa tcataacaga aatgccagcg gaaaaattat
     8281 cagcacagat tatggcctga tgcagattaa ttccacacat attcccggac tgaaaagaat
     8341 gggaattatc cgaagtgaaa atgaactgct cagtaatgtt tgcctgaata tacagacagg
     8401 tgcatggatc cttgcccggc attttcagca gtgtggtgtg aactgggagt gtctcggtag
     8461 ctacaacgcc ggatttaata aaaataacac tcaccgcaga atgaaatatg cccggctgat
     8521 ttatgccgca tatatgcagg gacgttaata acggagaata cgagccatgt tcctcatcat
     8581 cttactcgtg atctccgcta cgctgaccat acagttactt gccatcagac tggccggaca
     8641 ttatctcatg caggtgcata ccctccggcg tccacgtcac ctctccctgc tctgtgctgt
     8701 tctggcactc accgccacca ttacgcacat catacggggg acagacatta ccggactttg
     8761 ctttctgttg ctgatggccg gctgcatgac actgatgaca ctgactgatg cagcctcctg
     8821 ccgtcttccc cgtcccttca ccctcacatt cctgatgctg ggtgtggcgt ttcgtttatg
     8881 gcagggcgat gctgctattg cgcttgcctg tgctctgttc tggtttctcc tgttatctct
     8941 cctgaggcgc tacacctcat accgcaaggg agtggagacg ctgggactgg gggacgtgtt
     9001 tctgattgca ggtgtggcca tgtggtcatc tgccaacagc actccgtgga tcatcggtat
     9061 tgccgcagcc agcacaatga tctgtatgtc gctgttcccg cgcagtaata ctggggccgc
     9121 tattccgttc ggtccgttta tctgtgcatc actctacatt ttcacttttt ttcctggtgc
     9181 catttttcac accggagggt aacaccatgc aaaaagacga cgataaaggt tttgttctgt
     9241 ttgaaattct ggccggactg attgtgatcg gtatcgccac accaatgatt tattcagaaa
     9301 ttgaaaactg gctgaatgag cagctttacc agagtgctgc atatcatgca gatgcctaca
     9361 ataccgcagc cagaaactat attgccgaca acaatgcccg tctgcatagc ggcagccttc
     9421 cggcaaattt cacggctgat gacctgattc gtcagggata tctgaaacag gggtttaacc
     9481 attctccctt tggtcagagt tatatcaccg gtatccgccg taaccagacc accgggcgtc
     9541 tggaagccct gacctgctca actggagggc agacaatcaa agaagaaggg ctgcgtagtg
     9601 tggcgggtca gttgccgggg ctgggtggat ttatcagcaa aaatggaacg gcaacaggcg
     9661 cattcggagc ctggacagat aaaccgggag actacggact tacctgttcg acaggtcaca
     9721 tcgccgtcgt catgagcggt gatgacctgc aggaaagtga ccgtctctac cgttttcagg
     9781 tagcaggtcg tcctgaactg aatcagatgc ataccgcaat caatatgggc gggaataaca
     9841 taaacaacac cggcaatatc aatggtcaga gtgccacact gaaaggtgat attacgagtg
     9901 aagacggctg gctaatcacc agaaacaata agggatggat gaatatcaca catggtggtg
     9961 gattcaccat gacggacagc cagtggatac gcgcggttaa tggcaaaggc atcacaacca
    10021 ccggggaaat caaagggggg aaagtcagtg gtggtacagt ccggtctgac ggtcgtcttt
    10081 caaccgggga gtatttacag cttgataaaa cagctacggc aggcacaaag tgttcaccgg
    10141 acggacttgt aggccgcacc agtacagggg ccatactttc gtgccaatcc ggtatgtggg
    10201 caggttttga atcctaccct gtcggaagcc ctataccgtg gccatcagcc acccctccac
    10261 aaggctacct ggtgatgaac gggcagtcat tttcatgttc ccggtatcct cagcttgccc
    10321 gtgcttatcc cgggtgtaag ctgccggatt tgcgcggtgt ttttatccgt ggctgggaca
    10381 acggacgtgg gctggatgga gatcggaaca gacatctcct gagttatcag gccgatcaat
    10441 ccggtgtcta tcatgagcgt gggggatggc tgaaagggca tcacagcggt atgccttact
    10501 gggcccaggg aagcacaacg gaaatgcgcc ccaaaaacat tgcctttaac tacatcgtca
    10561 aagcaagctg atgcatttaa ggcgtattaa cttttaactt acagcaacgt attttgaccc
    10621 agaccatgct tcccgaatac cccggtttac ctctgtacgc tctgaatcct gccgcaccat
    10681 agcctgcttt gccgtcacag acggcaaagc cgtttgagcc actaccccag ttcgcataaa
    10741 ccggttttct ggagtcttct tctgcgtcat aacaggccga ataatcaaaa accagtgcat
    10801 cgctgatatt attcttccag acaccggatt ggcacgctgg ctactgcctg acgcttaatt
    10861 aaataccggc cccaggtata ttgagacacg taacagatat cgttccacct gactcataat
    10921 taccccacgc ataattattg acataccaga cattattttt atcggaataa acctgctgaa
    10981 aaccaccgaa aggagaacca ttaagatacg tccatgcaca atatgcatgg acaccaagtc
    11041 tgaaattacg aatatttttt ccgatgttat aggtctgcgt tgtgaattta atttcagttg
    11101 accgccagac accggattgg cacgaaagta ttgcatttct gtatccagcc tcctaccttc
    11161 cccgttttta tcgtggaagg cagatatgtt tcaaaaaaaa actcgcaaaa tgtccctcag
    11221 ccgggcactg gacaaatacc tgaagactgt ttccgtccat aaaaaaggtc atctgcagga
    11281 gttttatcgc gtcaatgtga ttaaacgtca ccctatggcc gatcgctata tggatgaaat
    11341 cactaccatt gatatcgccg gttaccggga tcagcgttta gctcaaatta acccacgaac
    11401 agggcgtcaa atcaccggaa ataccgtccg tcttgaactt gcccttttat catcattatt
    11461 caatatcgcc cgtgtggaat ggggaacatg ccgtatgaat cctgttgaac tggtccgaaa
    11521 accgaaaatc agtaatggcc gcgaccgccg actgacttct ggtgaagaac gccgcctttc
    11581 ccgctatttc agggacaaaa atccgcagct ctacgttatc ttccatctgg ctctggaaac
    11641 agcaatgcgc cagggagaaa ttctctcatt acgctgggaa cacctcgatt tgcagcacgg
    11701 cgtggcgcat cttcctgaaa cgaaaaatgg ctcaccacgc gacgtaccac tctcccggaa
    11761 agcacgtaac tacctgcaaa ttctgccaca acagataaac ggcaatgtgt tcagttacac
    11821 ctcgtccggt ttcaaaagcg cctggcgtac cgctcttctg gatctgaaaa tagaaaatct
    11881 gcattttcac gatctccgcc atgaggccat cagccgtctg ttcgagctcg gcacactgaa
    11941 cgttatggag attgcagcca tttccggtca ccgctccctg aacatgttga aacggtacac
    12001 acatctccgg gcatatcacc tggtcagtaa actcgatgca aggcgtaaac agaccagcaa
    12061 aatttcccct tattttgttc cctaccctgc aactgtcaga aacagaaacg gagtgttcat
    12121 tgtgacactg cacgatttcg accttgaaag ccgtgctgaa acccgggaac tggcaatatc
    12181 tcacgccagc gtattgttgc tgcgcgcact ggcccaggct gctcagcggg gtgaacgcgt
    12241 ccccacacca ggcgagctac ccgcaaacat tgatgaacgg gtcatgattt gtccgttaac
    12301 aaacagccag ttcatgacaa aagcgaacag tattttcata tcctcctcct gaacaaataa
    12361 tttttgttca aaattaacgc tatgagaccc tttatgaaac acttatcctc ccgtgcagcc
    12421 actgcggata ataatcgtat cgttacaatt gaagacgttt acacaatcgc attctcgcaa
    12481 ccaattcaca ccgaatcagc gttggggcaa aggtggatgt tgatccatac tggtgaatat
    12541 gaaagtgcca gcgatgcaaa acgcttcctt gatacgctcg tcaccgggca ctgtgcttgc
    12601 atggtaacac catcacctgt tctggatcat gacacctggc tgcgtatccg cctgctgatg
    12661 ggcgagataa gggatgccaa ataaacattc atggccccaa cacataaaaa taacgccggg
    12721 gactctggca gaggtaccat tctttttaca tagagccaat at
//
LOCUS       DAZGAP010000160        11975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGAP010000160 DAZGAP010000000
VERSION     DAZGAP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.161"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(308..9445)
                     /locus_tag="ACHLAM_003994"
     CDS             complement(308..9445)
                     /locus_tag="ACHLAM_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO7855450.1"
                     /translation="MNRNCYRIIFNKARGMLMVVADIARSGRAGASPSSRTGYPRRQR
                     ICRVTPLAFSLWLASGMVHSVSAAGIVADHGAPGHQQPTIMQTASGIPQVNIQTPSAG
                     GVSHNTYSQFDVGNQGVILNNAHNNVQTQLGGMVAGNPWLAKGEARIILNEVNSRNPS
                     QLNGFVEVAGKKAQVVIANPAGISCDGCGFINANRATLTTGQPQMKNGSLTGFRVEGG
                     EIQVTGKGMDASRTDYTDIIARSVKINAGVWAQDLKVTTGRNNVDIAHGQTEKKAADA
                     SSQPQVALDVSSLGGMYAGKIRLVGTETGVGVRNAGHIGAQAGAVTLTADGRIENSGS
                     ISAKTDVHLATTRELNNSGSVYAGQDTQIQSDGVFTHTGSVASRRNTRIQTTRLTGGE
                     RSLLAAGVKDDGRLAEAGNLTVSTTGELAAHGQVLSGGDMQLKGQGLDLSNSRIQGQH
                     TGLDAGSGNLSTQNAQLSAGTFAARTAGQFSNNGGTINAGTLQISAQSLSNHKGSLIQ
                     TGTGDFSLSLPGSVDNREGLLAANGAVRLDALSLDNRRGKVQAAQSGSLQVKTTGAVD
                     NLQGTLIASRDILINTQALNNDNGLISAAAGTGRIKTQQAVSNTAGRMESAGRLDISA
                     GNLNNHQGTVVSDGLSVTLDGALDNTSGRLLSQKTLSVSGSELVSDDGLIQSGSDMTL
                     DVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNNQQGFMAGQKDVTLNTGTLDNRQGAL
                     GSQASLQVSSGTLMNQKGALKAGTDMLLSGDDVSNQEGTLAAGGDLNAHLNVLENQQG
                     TVVSNGNSRLDVTRSDNQGGRLVAQQSLTLSSTDIINDASGLIQSGASLNLRADTLSN
                     RNSGDRGGVISQGPMTLNAGTLDSTAGVLLSGDALSLTAGVVNNTSGQVVANGLLGWN
                     SQALNNQSGLIQGKGISINTAGQTLDNRGGTLNSLQELTVSTGAMDNRGGTVGAKTTA
                     DLSTTSLDNREGGRLVSEGELRLHTGGLQNSHGQIQSVGDILFDSVRGVVDNVSGLIR
                     SGSAITLNALQFINRHTQNTGQGLEAQTIHITTQDLDNQTGSIVADRVLTVMADRTLS
                     NNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQSVSVTAGQLGGDGKLLSPGDITLKSN
                     TAFSNSGQTIANGNLTLSVNGDVTNTGSLLAGSRLDLNSIRLENTEKGEISAGQTWLN
                     VTDTLLNRGLIDGKYTRLQANTLTNSGTGRIYGDAVGVSAATFNNLEENGVAATLAGR
                     ERVDLGVQTLNNRTHSLIYSAGDMYIGGTLDANGSATGKAGVLNNHSATVEATGSLAL
                     SAGQINNVNDHFTTEHVVVSTEKVTEYQLSGSDKRWRAGEPDVYVDNDSSNSLKKLHT
                     PEGARDKFTQYDYTRTVEETRVKDSDPGKILSGAGMTIVADKLLNDKSQVVAGGLLTI
                     PSGSVENVSVSGERHVTDSGTSTYYYRIRKKGKDKQGEKTSQYTPPTVIQSITLKPGE
                     LTSHGQIQGSQVTLSPLKPQGTDVQTGQAGNVDAAVAATDRIPLRPVVSAGEPVILLP
                     GQQFEVSTPQGSIHVAGPDTRLPDSSLFKTNPAVNVPYLVETDPRFTNQKMWLGSDYM
                     QKAFSQNGDNMLKRLGDGFYEQRLIREQVVALTGQRYLDRYGSDEEQFKALMDAGIAF
                     GKQYNLKPGVALTAEQMALLTGDIVWLVNTTVTLPDGSTQTVQVPQVYARVKPGDVNS
                     AGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLSAENIHNQAGSIQGANVSLTARTDIN
                     STGGLLQATDSLLAMAGRDISLTTTTRTAQRDAGQNHFERTSIDSVAGVYVQNDQGRL
                     VLQAGRDMNLTAATVVNQGKDSLTQLSAGRDMTLSTVTTSAQDNIIWDKNNRLSQGVT
                     QSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKGLALMAGHDVTLTGAQNTRSLDEYHK
                     VTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDTVSISAGHNLNVTGSSVAGDNRVSLA
                     AGNDLSIGTLTESNRETHLKQEKKSGLMSSGGVGFSVGSQSLKVTDTATDSTQKGSTV
                     GSVHGDVSLQAGNRLSANSADLIAGRDMALSGKEVSISAVTDRHVQTHSVEQKTSGLT
                     LALSGTVGSALNTTVETVQAAKSAGNSRLEALQGVKAALSGAQAVQAGRLAEAQGNQK
                     ENNNAVGISLSYGSQSSKSEQQSEQTVAKNSALTAGNNLSIQATGSGVKDVDGDLTIQ
                     GSQIKAGNNVLLQANRDVNLVSAENTSKLEGKNSSSGGSVGVGIGVGAGGWGISVSAS
                     ANQGKGSEKGNGTTHTETTVDAGNRLTIISGRDTTLTGAQAGGETVKVDAGRHLTLTS
                     EQDSDRYDSKQQNASAGGSFTFGSMSGSASVSLSRDKMHSNYDSVQEQTGIFAGRGGF
                     DVTTGQHTQLNGAVIASTATADKNRLDTGTLGFRDIENRADYKVEHQSVGISTGGSIG
                     GQFAGNMANNLLVGANHEGHAESTTQSAVSAGNITIRDTQSQKQDVADLNRDAAHANQ
                     TLSPIFDREKEQQRLQQAQLIGEIGNQVADIARTEGQIAGEKAKRDPAALNQAREELE
                     AAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQAIQAATAAVQGLAGGNLSAALAGGA
                     APYIAEIIKQTTPDGAGRVAAHAVVNAALSLAQGKNALAGAAGAATGEVVGMIATQMY
                     GKPVSELSETEKQTVSTLATVAAGLAGGLVGDSGASAVAGAQSGKTTVENNALSFGDG
                     FESNAAAATSWNKYAVDNNLTPEQTQAGLDKIAKGDMPDSTNITKVIVDGYQDGVMIA
                     GAWYLGPAASVGKMVSGGVVAEIANGSYQWFDLSQPGNENKNWDWKSSASSGIAGMLA
                     PGRTVGQNVGIAMGGAFFTDGPNTGSIGGAAAGAGLGGAFGVYAPGIVNSVTGKEFPG
                     FIFDATGSFGSEFLGGYIKEASSNNQFSSSAKKEEGNDCLHCIVHICMPLLYHQWISF
                     DGGRSFIYLPNKRILFFPDPPNDKSYCVWVHSRYIDNTSSDSF"
     gene            complement(9461..9988)
                     /locus_tag="ACHLAM_003995"
     CDS             complement(9461..9988)
                     /locus_tag="ACHLAM_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO7855451.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAVVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKNRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            complement(9998..11077)
                     /locus_tag="ACHLAM_003996"
     CDS             complement(9998..11077)
                     /locus_tag="ACHLAM_003996"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO7855452.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTATWRLGLQHRHYISQATLDAGVSWQRGTRWSGAQPAPEETFGEATALSKILHINA
                     SFNLPFTLAEQPFSYFFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQALYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRGRLLSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFLNF"
     gene            complement(11075..11650)
                     /locus_tag="ACHLAM_003997"
                     /pseudo
     CDS             complement(11075..11650)
                     /locus_tag="ACHLAM_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
BASE COUNT         2467 a         3697 c         2875 g         2936 t
ORIGIN      
        1 actatatagt tgtttcattt tttcttatcc tctgacgaac ctttcggttt attgagttat
       61 tggctccttc aggtaactca cctttggcaa gtttatccag tcctgcctgc ttctgttccg
      121 aggtgagatt attggcatca gcatactgat tccatgagga aaccgcctga ccataactca
      181 ccattcctga tggaagactg agcgagttat tctcaacaac aatcctaacc gcaaaggttg
      241 ggattgttgt taatctcact caggatctga tggaggtttt ttacgagaat taaacttatc
      301 gatcagatta aaaactatcg ctgctagtgt tatcgatgta cctgctatgc acccaaacac
      361 aataactctt atcatttggt gggtcgggaa aaaaaagaat ccttttgtta ggtaaataaa
      421 taaagctcct ccccccatca aaagaaatcc actgatgata caaaagaggc atgcatatat
      481 gaacaataca atgtaagcaa tcattcccct cctccttctt cgcgctagaa gagaactgat
      541 tattactact tgcttctttt atatatccac caagaaattc actaccaaaa gatcctgtcg
      601 catcaaatat aaatcccgga aactctttcc cagtaactga gtttactatt ccgggagcat
      661 atactccaaa tgctccaccc aatccggccc cggcagcagc ccctccaata gagcctgtat
      721 ttggaccatc ggtaaagaat gcaccaccca tcgcaattcc gacgttttgt ccgacagttc
      781 tccccggcgc aagcataccc gcaatgcctg aactggcact gcttttccag tcccagtttt
      841 tgttttcatt acctggctgg cttaaatcaa accactggta acttccattg gctatctccg
      901 cgacgactcc accactgacc attttaccga ccgaagcagc aggccccaga taccatgccc
      961 cggcaatcat tacaccatcc tgatatccat ccacgatcac tttcgtgata ttggtgctgt
     1021 ccggcatatc acctttcgct atcttatcca gtccagcctg tgtttgttcc ggtgtcagat
     1081 tattgtccac cgcgtacttg ttccatgatg tagccgcagc agcattgctt tcaaaaccat
     1141 caccgaagct gagcgcatta ttctcaaccg tcgtcttacc tgactgtgct ccggctaccg
     1201 ctgaagctcc cgaatctccc accagaccac cggccaggcc ggcagcaacc gttgccagtg
     1261 tcgagaccgt ctgcttctct gtctcgctca gttcactcac cggcttaccg tacatctgtg
     1321 tggctatcat tcccaccacc tcgccggtgg ctgcgcctgc ggcaccagcc agagcattct
     1381 taccctgtgc cagagacagg gctgcattca ctactgcatg agctgccaca cgtcccgcac
     1441 cgtccggggt ggtctgtttg ataatttcag caatatacgg tgctgcacca cctgccaggg
     1501 ctgcactcag attaccgccg gccagcccct gtactgctgc cgttgcggcc tgtatcgcct
     1561 gctggtattt gcctcccgtt ccaaaacctg aggccgccat accgttattg taggcccgct
     1621 gcgccacatc ctgttccgtg aacggttttc ctgctgcctc aagctcctca cgggcctgat
     1681 tcagtgctgc cgggtcccgc ttcgctttct caccagctat ctggccttcc gttctggcaa
     1741 tatctgccac ctggttacca atctcaccaa tgagctgcgc ctgctgcagt cgctgctgct
     1801 ctttttccct gtcaaagatg ggggacagcg tctggtttgc atgtgctgcg tcacggttca
     1861 ggtctgccac atcctgtttc tggctctgcg tatcccggat ggtgatattc ccggcagaca
     1921 cagccgactg agtggtgctt tctgcatggc cttcatgatt tgcaccaacc agcagattgt
     1981 tggccatatt tcccgcgaac tggccaccga tgctgccgcc agtgctgata cccacgctct
     2041 ggtgttcaac tttgtaatca gcacgatttt cgatatcacg gaagcccagt gtaccggtat
     2101 ccagccggtt tttatctgcc gttgccgtgg acgcaatcac cgcaccgttc agttgcgtat
     2161 gctgtccggt ggtgacatca aacccgcccc tgccggcaaa aatccccgtc tgctcctgca
     2221 cactgtcata gttactgtgc attttatccc ggctgaggct cacactcgcc gaaccgctca
     2281 tggacccgaa agtgaagctg cctccggcac tggcattctg ctgcttactg tcataacggt
     2341 cactgtcctg ctcactggtc agtgtcagat gacgaccggc atccactttc accgtttcac
     2401 cgccggcctg cgcgccggta agcgtggtgt cccgcccgct gataatggtc aggcggtttc
     2461 ccgcatccac cgtggtttct gtatgggtgg taccgttgcc tttttcactg cccttaccct
     2521 ggtttgcgct ggctgaaaca ctgatacccc agcctccggc ccccacaccg ataccgacac
     2581 ccacactgcc gccactggat gagttctttc cttccagctt tgacgtgttt tctgctgata
     2641 ccagattcac gtcacggttt gcctgcagca gaacgttgtt ccctgccttt atctgactgc
     2701 cctgtatcgt caggtcacca tccacgtcct tcacgccgga gccggtggcc tgaatactga
     2761 ggttattacc tgcggtcagg gcgctgttct tcgccaccgt ctgctctgac tgctgttctg
     2821 atttcgaaga ctggctgccg taagagaggc tgatacccac ggcattgtta ttctctttct
     2881 ggttgccctg tgcttctgcc agtcgtccgg cctgcacggc ctgagccccc gagagcgcgg
     2941 ccttcacccc ctgcagggct tcaagccggc tgtttccggc agatttcgcc gcctgcacgg
     3001 tctccaccgt cgtgttcagg gcgctgccca ctgtgccgga cagggcaagc gtcagaccgg
     3061 aggttttctg ctccaccgaa tgcgtctgca catgccggtc tgtgacggcg ctaatgctga
     3121 cctccttacc actgagtgcc atgtccctgc cggcaatcag gtcagcactg ttggcgctca
     3181 gccggttacc tgcctgaagg gaaacatcac cgtgaacact ccccaccgtg ctgcctttct
     3241 gtgtgctgtc tgttgcggtg tccgtgactt tcagactctg gctgcccaca ctgaatccca
     3301 cacctccgga gctcatcagc ccggattttt tctcctgttt caggtgtgtt tcacggttgc
     3361 tttctgtcag cgtgccgata ctcaggtcat ttccggcagc cagggagaca cggttatccc
     3421 cggccacact gctgcccgtg acgttcagat tatgtccggc actgatactg accgtatccc
     3481 cgttcagttc actgcccgtc atggtccggc ggtcagtgac atcatgcgtg gttgtggtgg
     3541 ttttcgataa catcccgctg ctgccggtca ctttatggta ttcatccagc gagcgggtat
     3601 tctgtgcacc ggtcagcgtc acgtcatgac ctgccatcag ggccaggcct ttctgagctg
     3661 acagcgatgc cgcctgtgac gtcatatccc gtccggcggt cagcgtgaca tcaccgttac
     3721 cggcaagcgt gctgcccgta ctctgtgtga cgccctgcga cagacgatta tttttatccc
     3781 agataatatt gtcctgcgcg gaagtggtca ctgtggaaag cgtcatatcc ctgcccgcac
     3841 tgagttgcgt caggctgtcc ttaccctgat tcaccaccgt tgcagccgtc agattcatat
     3901 cccgtccggc ctgcagcacc agtcttccct ggtcgttctg cacatacacg ccggccacac
     3961 tgtcgatact ggtacgttca aaatggttct gtccggcgtc gcgctgtgcc gtacgggtgg
     4021 tggtggtcag actgatatcc cgtccggcca tcgccagcag gctgtccgtg gcctgcagca
     4081 gcccgccggt actgttaata tccgtccggg ccgtcaggct gacatttgct ccctgaatgg
     4141 agccggcctg gttgtgaatg ttttctgcac tgagctgcac ggtctgcttc ccggccagtt
     4201 taccgctgtt aaacaggtca ccatccagct tcatcaccat gtccctgccg gcaatcagcg
     4261 caccggcact gttcacatca ccgggtttta cacgggcata cacctgaggt acctgaactg
     4321 tctgcgtgct gccgtccggc agtgtcaccg tggtgttaac cagccagaca atatccccgg
     4381 tcagtaacgc catctgctca gccgtcagcg ccacacccgg tttcaggttg tattgcttac
     4441 caaatgcaat acccgcatcc attaatgcct taaactgctc ttcatcactg ccgtatctgt
     4501 ccaggtaccg ttgcccggtc agagctacaa cctgctctcg aatcagacgc tgctcgtaaa
     4561 aaccgtctcc cagacgtttg agcatgttat ccccgttctg agaaaacgcc ttctgcatgt
     4621 aatcactgcc cagccacatc ttctggttcg taaaacgcgg gtccgtttca accagatatg
     4681 gcacattcac tgccggattt gttttaaaca gactgctgtc cggcagacgg gtatccggcc
     4741 cggcaacatg gatactgccc tgaggtgtgc tgacttcaaa ctgctgcccc ggcaacagaa
     4801 tgaccggttc accggcagac accaccgggc gaagcggtat ccggtcagta gcggctactg
     4861 ccgcatccac atttccggcc tgtcctgtct gtacatccgt tccctgtggc ttcagaggag
     4921 aaagcgtgac ctggctgccc tgaatctgtc cgtggctggt gagctctccc ggcttcagcg
     4981 taatgctctg aattacagtg ggcggagtgt actgagaggt tttctccccc tgcttatcct
     5041 ttcctttttt cctgatacgg taataatacg tggatgttcc tgagtccgta acatgccgct
     5101 cgccactgac actgacattt tccacgcttc ctgacggaat ggtcagcagg ccgcctgcaa
     5161 caacctgact cttatcatta agcagtttgt ctgccacaat cgtcatgccg gcaccggaca
     5221 gaatttttcc cgggtcggag tcttttaccc gggtttcttc cacggtacgg gtgtaatcgt
     5281 actgcgtgaa tttatctctg gcaccttccg gagtatgcag ttttttcagt gagttactgg
     5341 aatcgttatc cacataaacg tccggttcac cggcgcgcca gcgtttatct gagccgctga
     5401 gctgatactc agtcaccttt tccgtggaaa ccaccacgtg ctccgtggtg aaatggtcat
     5461 tcacgttatt gatttgtccg gcgctcagtg ccagggaacc ggtggcttca acagtggcac
     5521 tgtgattatt cagtacaccg gctttacctg tggcgctgcc attcgcatcc agcgtgccac
     5581 cgatatacat atcgcctgca ctgtaaatca ggctgtgcgt ccggttattc agggtctgta
     5641 cccctaagtc cacacgctca cgcccggcca gcgtcgccgc cacgccattt tcttccagat
     5701 tattaaatgt ggcagcactg acaccaacag catcgccgta gattcgcccg gtaccggaat
     5761 tcgtcagggt gttcgcctga aggcgcgtat atttcccgtc aatcagccca cggttcagca
     5821 gtgtgtccgt cacgttaagc caggtctgcc cggcactgat ttcacctttc tctgtgtttt
     5881 caaggcggat gctgttcagg tcaagccggc tgcccgccag caggctgccg gtattagtga
     5941 catcgccatt aacggaaagc gtaaggtttc cgttcgcaat agtctgacca ctgttgctga
     6001 acgctgtgtt acttttcagc gttatatcgc ccggcgaaag cagtttcccg tcaccaccaa
     6061 gctgaccggc tgtcacggat actgactgcc cggccttaac cacaccgtca cggttcgaaa
     6121 acgccagctg acggccactg acagacaatg tcgcgccgga agagagcacg ccatcattat
     6181 tactgagcgt cctgtctgcc ataaccgtaa gcaccctgtc tgccacaata ctgcccgtct
     6241 ggttatcaag gtcctgcgtt gtgatgtgta ttgtctgcgc ttccagcccc tggccggtat
     6301 tctgcgtgtg gcgattgata aactgcaggg cgttcagcgt aatggcagaa ccactgcgaa
     6361 tcagaccaga gacgttgtcc acaacgcccc gtacactgtc gaacagaatg tcgcccacag
     6421 actgtatctg tccgtggctg ttctgcaggc ctcccgtatg cagacggagt tcaccttcac
     6481 tgaccagccg gccaccctca cggttatcca gcgaggtggt gctcaggtca gctgtcgttt
     6541 tcgcccccac ggtcccgccc cggttatcca ttgctccggt actgacagtc agttcctgca
     6601 ggctgttcag cgtaccgcct ctgttatcca gcgtctggcc tgcggtatta atgctgatgc
     6661 ctttcccctg aataagacct gactgattgt tcagcgcctg gctgttccag ccgagcaggc
     6721 cgtttgccac cacctgaccg gatgtattgt taaccacgcc tgcagtcagg gacagagcat
     6781 ctcccgacaa aagcacaccg gcagtgctgt ccagtgtgcc ggcattcagc gtcatcggcc
     6841 cctgactgat aaccccgccc cggtcaccac tgttacggtt actcagtgtg tcagcacgga
     6901 gattcagcga tgcaccgctc tgtatcaggc cgctggcatc gttgatgata tccgtcgagg
     6961 aaagggtcag tgactgctgt gccacaagcc tgccaccctg attatcagag cgggtgacat
     7021 ccagtctgct gttaccgttg ctgaccaccg tcccctgctg gttttccagc acattcaggt
     7081 gcgcattcag gtctccgccg gcggcaagcg tcccttcctg attactgaca tcgtcaccac
     7141 tcagcaacat atccgtcccg gccttcagtg cgcctttctg gttcatcagc gtacctgacg
     7201 atacctgtag tgacgcctgg ctccccagcg cgccctgacg gttatcgagc gttccggtgt
     7261 tcagggtcac atccttctga cccgccataa atccctgctg gttattcagc ataccggccc
     7321 gtactgtcag ggtaccggcg ctgctgatac caccccgtgt tttcgtgttc cggttactga
     7381 gtaccccgtc ctgtacatcc agcgtcatgt cactgccgga ctgaatcaga ccatcatcag
     7441 agaccagttc gctgcctgac acagacagcg ttttctgtga aagcagtctg ccggacgtat
     7501 tatccagcgc accatccagc gtcacagaca gcccgtcaga caccactgta ccctggtggt
     7561 tattcagatt ccctgccgaa atatccagcc tgccggcgga ctccattcgt cccgccgtat
     7621 tgctgacagc ctgctgtgtt tttatcctgc ccgttccggc agcagctgaa atcagtccgt
     7681 tgtcgttatt cagcgcctgt gtattaatca ggatgtcacg gctggcaatc agagttccct
     7741 gcaggttatc cacggcgccg gttgtcttca cctgcagact gcctgactgc gccgcctgca
     7801 ctttccccct gcgattatca aggctcagtg catccaggcg caccgcgcca tttgccgcaa
     7861 gcagcccttc ccggttatcc acgcttcccg gcagactcag tgaaaaatcc cccgttcccg
     7921 tctgaatcag gctgccttta tgatttgaca ggctctgtgc cgatatctgc agtgtgccgg
     7981 catttatcgt gccgccatta ttgctgaact gcccggcagt ccgggcagca aaggtaccgg
     8041 cgctcagttg cgcattctgt gtggacagat ttccgctgcc tgcatccagc ccggtatgtt
     8101 gtccctgtat ccggctgttg cttaaatcca gcccctgtcc cttcagttgc atgtccccac
     8161 cggacagaac ctgtccgtgt gccgccagtt cgccagtggt actgacagtc aggttacccg
     8221 cttctgccag acgtccgtca tctttcaccc cggcggccag cagactccgc tcaccgccgg
     8281 tcagacgcgt tgtctgaatc cgggtgttgc gacgtgatgc cacagagcct gtatgggtga
     8341 atacaccatc actctgtatc tgcgtatcct gccccgcata aacagaacca ctgttattca
     8401 gttcccgggt tgtcgccaga tgcacatccg ttttcgcact gatactgcca ctgttttcaa
     8461 tacgtccgtc agccgtcagg gtaaccgccc cggcctgcgc accaatatga ccggcgttac
     8521 gcacgcccac acctgtttcc gtaccgacaa ggcgaatttt tccggcatac attccaccaa
     8581 ggctggacac atccagcgcc acctgtggct gcgaagaggc atcagcggct tttttctcag
     8641 tctgcccgtg tgcaatatcc acattgttac gtccggtggt cactttcagg tcctgcgccc
     8701 agacacccgc attaatcttc acagaccggg caatgatatc ggtataatcc gtgcgcgagg
     8761 catccatccc tttgcccgta acctgaatct caccaccttc aacacggaat ccggtcagac
     8821 tgccgttttt catctgcggc tgccccgtgg tcagcgtggc ccggttcgca ttaataaagc
     8881 cgcacccgtc gcaactgata ccggccgggt ttgcaatcac cacctgcgct tttttcccgg
     8941 caacctcaac aaaaccgttc aactggctgg gattgcgact gttcacttca ttaagaatga
     9001 ttctggcctc tcccttagcc agccacgggt tcccggccac catgccaccc agctgagtct
     9061 gcacattgtt atgggcatta ttcagaatga caccctggtt accgacgtcg aactggctgt
     9121 atgtgttatg tgacaccccg ccggcagaag gcgtctgaat attcacctgc ggaatgccgc
     9181 tggccgtctg cataatcgtg ggctgctgat gccccggtgc gccatggtct gcgacaatgc
     9241 ctgcagcgct gacagaatgc accatccccg aggccagcca cagggagaat gcaagcggcg
     9301 tgacacggca gatacgttgc ctgcgtggat atcctgttcg ggaggagggt gatgcaccgg
     9361 cgcgaccgga acgggcaata tctgccacca ccatcagcat tccccgggct ttgttaaaaa
     9421 taatgcggta acaatttctg ttcattctga taaacctgac ttatctctcc ggcacatcca
     9481 cagcaagcgg gtgctgcaca cgccactgtc gcgcctcttc acgactgacc tgcgcacccc
     9541 ggaaattcat cacccgcaac ccggtctgct gcccacggtg gtacagcgaa cgccagcaca
     9601 gacggggaaa aatatcctgc cggacaagac gactcatatc cggagtgtgt ccaaaagggg
     9661 caacccagtc acagacccac atccggtcgc cgctgttcca gtccgactga ggcatgagaa
     9721 ttgccggctg cgtaagataa cgcgcctctg cctccgggct gagccaggcc tggctcatga
     9781 agaaaacagg tcgcccctgc tctgttgcca caacatactg ccggttctta atcaccggca
     9841 gcagcaggtc cggcagcgta tgcagtggtg catcacgatg caggggagag tgcatccaca
     9901 accacacgac cgctcccagc acttccgctt cattgaccgg ctcaccggaa tacagggggc
     9961 tcagaacgtc atatttcccg ttacgcatga acgtttctca gaaattcagg aacagattaa
    10021 aaccggctgt cacatcactc gtcataaaac ccgccggttt tgaaaacgga cgaccgatga
    10081 aaacgtcata atccgtactg agcagtcgcc ccctcagccc cacaacacca ccggccagat
    10141 gacgtcccac caggtattca ctgccggctc cgcccacctc gccataatcc attcccagat
    10201 ataacgcctg ctccggcagt ggcgtacgcc aggccaggtc attgcggata taccagccgt
    10261 tactggcatt cagcgttctc tccccgtcaa aaccacgaac cgtccagcgg ttaccgatgg
    10321 caaactgttc ctgtggagtc agcggggtat tgctcagctg gcgctgatac cggaagaaat
    10381 aactgaaggg ctgctctgcc agtgtaaagg gcagattaaa ggatgcattt atatgcagga
    10441 tcttactgag cgccgttgcc tcgccgaacg tttcttccgg tgccggctgt gcgccagacc
    10501 agcgggttcc ccgttgccag ctgacgccgg catccagtgt ggcctgcgag atataatggc
    10561 ggtgctgcag tccaagtcgc caggtcgccg tctgccggcg ctgtacctgg atttccgtat
    10621 cattaatgta gttacgcgac ctgcgggcct gaacatcgta agtcagcacc gttttctgcg
    10681 tcccgttacg atgcagtacc cggctcagct gcatgcccag attctgactc tcgccactgt
    10741 actggtaatc ctcaaccgca ccagctaccg tctgatggta atcgtaatca ctggcggtga
    10801 cggaaaactg ccagtaaccg aaaggcacag agtaatgggc cgtgtagttt ttactgccct
    10861 tacccccgta atgtggaaaa ttatgggtgg cagaaacata cagcaggtca ctcagggaaa
    10921 aaggattatc cagagaaaat gttataccac cgagataacg ccctgtgctg cgggtacctg
    10981 aatcatcaag cgaaaacccc agtcgccagt atttatcctg tttacgggta ataagaatat
    11041 cactttcccc tggctgctca ccgggcacaa tttccatatc agcctgcaca cccggcagtc
    11101 gttgcatatt ctccagcccc tgctcaatgt cccgtaaatc cagcagagaa ccttcgtgtg
    11161 ccgggaatga ggaatacaac tgaatatagt catcactgtc cggtgtcaga cgcacatgtc
    11221 gcacaacacc gggaataaca accagccgga ggataccgct ttttaaatcc tgcgaaggtg
    11281 ccagaacacg ggtggtgaca taaccatgat cgacaagacg gttctgcaat gtactcatca
    11341 gcagattaat tcctttcgcc cccaggcaat gcccgaccgc cccgttggcg attttttgta
    11401 aaggcagcca gtgtggtaac gcatcagccc ccttcagttc cgtctgttta atctgaaaac
    11461 agggcgtttc aacaggaaaa ttaatcttat gggcaaaaga gccaggtgca gaaaggcgaa
    11521 catcaggggc tgaaggcgtc agttgttgct caagggcttc ctgacgctgc tgctgaatga
    11581 taaatgtatc ctctccacta cgccggatat ccgcggcctg aagagataaa ggcgataata
    11641 aaacagacat cagtgtcagt gataaaatga acggacagcg ccggaataag ctttgttcct
    11701 cagccctctc gttcctgcaa cataattttc cagccaaacg aaaaaccttt tatgcatgct
    11761 gtgtcaacaa tcaccagcct cctgacgatg tctgccgcca ttttggcaat aacacactac
    11821 cgcccttccg gaattccccg tatcaaccgg taccagaatg ctgagtaacc agtcagccaa
    11881 atcaggctgg tttaattttg ctcattatag aataatgatc tgaaaaatat caatataaca
    11941 ggtgtatcga tcgtaattgc agatgcttgc agatc
//
LOCUS       DAZGAP010000161        11745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGAP010000161 DAZGAP010000000
VERSION     DAZGAP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.162"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..341)
                     /gene="yehE"
                     /locus_tag="ACHLAM_003998"
     CDS             complement(60..341)
                     /gene="yehE"
                     /locus_tag="ACHLAM_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO7855453.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(604..1713)
                     /gene="apbC"
                     /locus_tag="ACHLAM_003999"
     CDS             complement(604..1713)
                     /gene="apbC"
                     /locus_tag="ACHLAM_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO7855454.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPNMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1845..3878
                     /gene="metG"
                     /locus_tag="ACHLAM_004000"
     CDS             1845..3878
                     /gene="metG"
                     /locus_tag="ACHLAM_004000"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO7855455.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEEIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4020..7808
                     /locus_tag="ACHLAM_004001"
     CDS             4020..7808
                     /locus_tag="ACHLAM_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO7855456.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDALDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDELQANNAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7818..11450
                     /gene="yehI"
                     /locus_tag="ACHLAM_004002"
     CDS             7818..11450
                     /gene="yehI"
                     /locus_tag="ACHLAM_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO7855457.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQHEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQARAVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPLMWSRPRLIINHQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYGLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11512..>11745
                     /gene="yehK"
                     /locus_tag="ACHLAM_004003"
     CDS             11512..>11745
                     /gene="yehK"
                     /locus_tag="ACHLAM_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO7855458.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDER"
BASE COUNT         3049 a         3008 c         2880 g         2808 t
ORIGIN      
        1 taaatcattc atgtgaatga tttaagcttt ctaaatccga ttaaaaccag ggtggcaaat
       61 taatcgtagc gatttaacac gattcgacgt ttgctatcgc tatctgcagc aatcacttcc
      121 gtcgttacac ctttaactgg cgaagaaacc tgatgacggg tatcaacatt atacgttaac
      181 tgaccacaat gctgttgagg ttgaccatta accatagcca tactgcatgt cgggggactg
      241 atcctaccat taatcgttaa tgtggcagtt tctgaagaga acgcgggaga ggccaatcca
      301 taggcaagaa aaataattcc tgacagccaa tacttattca ttgaacgtta tccctgtagt
      361 aaaggttatg cctggataga atgagtgcat aacaaactat agctgtacat ccactacaca
      421 gccacgaagg atgataatga agcattgcct gtacgatcaa tcgactttgt agagtttcgg
      481 acgaaggtcc gcagaatatt cgcagcatta aataagtgtt cattaatgcg gcaaaaacaa
      541 atacaccgca tcaatgtaac atctctgcgg tgtatgaaga ataaccagaa tggttaatac
      601 gcgttagacc gcgcggaagg aaatctcgcc tggaatgact tcaccctgcc agtaaagctg
      661 agctgcaacg cggtcagcca gctggcggta gatagcggta aattcgctct caggacggct
      721 gataaccgtt ggcgtgcctt tatcgagatc ctcacgtaag gagatgtgta acggcatctg
      781 ccccagcagc tgtgtgtggt atttctcggc cagtttctct gcaccaccgg taccgaaaat
      841 cggttcatgg tgaccacagt tactgcaaat atgcacactc atgttctcga caataccgag
      901 caccggaacc tcgactttct cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat
      961 gtcctgcggg gtggtgacca cgactgcacc ggttactgga atgttttgtg ccaatgtcaa
     1021 ttgaatgtca ccggtacctg gcggcatatc aagcaccaga tagtcgagat ccggccacaa
     1081 ggtttcctgc aacatttgca ttaacgcctt gctcgccatt gggccacgcc acaccatcgc
     1141 attgtcgtct gtcaccagat aaccaattga attagttgcc aggccatgag acataatcgg
     1201 tgccatatgg gtaccgtccg gcgaagttgg acgctgattt tctgcaccca gcatgttagg
     1261 gattgatggg ccgtagatat cggcgtccag aatgccgact ttcgcccctt ccgccgccag
     1321 tgccaacgcc aggtttaccg ccgtggagga tttacccacg ccacctttac cggaactgac
     1381 cgcgataatg ttcttcacgc cgttaattcc cggctgattc ttcacgcgtt tcagagtggc
     1441 tatgttatgc gacagtttcc agtcgatagc tttcgctccg gtgatgcgca gcagttcggc
     1501 gctacattgc tcttttaact cttcgaacgc gctatgccag acgaatggca taaccagttc
     1561 cacatgcagc gtgtcgtcca tccaggcgac atggtgcaac gctttcagcg ttgtgaggtt
     1621 atgcttcagg gttgggtgct gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc
     1681 ttctggtgat ttggcctggg attgttcgtt catcccgact ccttttttgt atagataaac
     1741 catcagttga tagtttacct gaagaatata gagaagtact tacttaacat tttcccattt
     1801 ggtactatct aagccctttt cactattaag aagtaatgcc tactatgact caagtcgcga
     1861 agaaaattct ggtgacgtgc gcactgccgt acgctaacgg ctcaatccac ctcggccata
     1921 tgctggagca catccaggct gatgtctggg tccgttacca gcgaatgcgc ggccacgagg
     1981 tcaacttcat ctgcgccgac gatgcccacg gtacaccaat catgttgaaa gctcagcagc
     2041 ttggtatcac tccggagcag atgattggcg aaatgagtca ggagcatcag actgatttcg
     2101 caggctttaa catcagctat gacaactatc actcgacgca cagcgaagag aaccgccagt
     2161 tgtcagaact tatctactct cgcctgaaag aaaacggttt tattaaaaac cgcaccatct
     2221 ctcagctgta cgatccggaa aaaggcatgt tcctgccgga ccgttttgta aaaggcacct
     2281 gcccgaaatg taaatccccg gatcaatacg gcgataactg cgaagtctgc ggcgcgacct
     2341 acagcccgac tgaactgatc gagccgaaat cggtggtttc tggcgctacc ccggtaatgc
     2401 gtgattctga acacttcttc tttgatctgc cctctttcag cgaaatgctg caggcatgga
     2461 cccgcagcgg tgcgttgcag gagcaggtgg caaataaaat gcaggagtgg tttgaatctg
     2521 gcctgcaaca gtgggatatc tcccgcgacg ccccttattt cggttttgaa attccgaacg
     2581 cgccgggcaa atatttctac gtctggctgg acgcaccgat tggctacatg ggttctttca
     2641 agaatctgtg tgacaagcgc ggcgacagcg taagcttcga tgaatactgg aagaaagact
     2701 ccactgccga gctgtaccac ttcatcggta aagatattgt ttacttccac agcctgttct
     2761 ggcctgccat gttggaaggt agcaacttcc gcaagccgac caacctgttt gttcacggct
     2821 atgtaacggt gaacggcgcg aagatgtcca agtctcgcgg cacctttatt aaagccagca
     2881 cctggctgaa tcattttgac gctgacagcc tgcgttacta ctacactgcg aaactctctt
     2941 cgcgcattga tgatatcgat ctcaacctcg aggatttcgt tcagcgtgtg aatgctgata
     3001 tcgtcaacaa agtggttaat ctggcgtccc gtaacgcggg ctttatcaac aaacgttttg
     3061 acggcgtgct ggcaagcgaa ctggctgacc cgcaactgta caaaaccttc actgatgccg
     3121 ctgaagagat tggcgaagcg tgggaaagcc gtgaatttgg taaagcagtg cgcgaaatca
     3181 tggcactggc tgacctggct aaccgctatg tcgatgaaca ggctccgtgg gtggtggcga
     3241 agcaggaagg tcgcgatgct gacctgcagg cgatttgctc tatgggcatt aacctgttcc
     3301 gcgtactgat gacttacctg aagccggtac tgccgaaact gactgaacga gcagaagcat
     3361 tcctgaatac ggaactgacc tgggatggta tccagcaacc gctgctgggc cataaagtga
     3421 atccgttcaa agcactgtat aaccgtatcg acatgaagca ggttgaagca ctggtggaag
     3481 cgtctaaaga agaagtgaaa gccactaccg cgccagttac tggcccactg gcagatgatc
     3541 cgattcagga aaccatcacc tttgacgact tcgctaaagt tgacctgcgc gtggcgctga
     3601 ttgaaaacgc agagtttgtt gaaggttctg acaaactgct gcgcctgacg ctggatctcg
     3661 gcggtgaaaa acgcaatgtc ttctccggca ttcgttctgc ttacccggat ccgcaggcac
     3721 ttattggtcg tcacaccatt atggtggcta acctggcacc acgtaaaatg cgcttcggta
     3781 tctccgaagg catggtgatg gctgctggtc ctggcgggaa agatatcttc ctgctaagcc
     3841 cggatgccgg tgctaaaccg ggtcatcagg tgaaataatc cccccttcaa ggcgctgcat
     3901 cgacagcgcc ttttctttat aaattcctaa atttgttttc ttgcgatttt gtctctctct
     3961 aacccgcata aatactggta gcatctgcat tcaactggat aaaattacag ggatgcagaa
     4021 tgagacactt tatctatcag gacgaaaaat cacataaatt ctgggcggtg gagcagcagg
     4081 gaaacgaact gcacatcaac tggggtaaag tcggcaccaa cggacaaagc caggtaaaaa
     4141 gttttgcgga tgcagcagcg gcggaaaaag cgggactgaa gctgattgca gagaaggtga
     4201 agaaggggta tgtggagcaa gcgaaggata attctttgca accttcccaa acggtaacgg
     4261 actctctcaa ggtagctgac ttatccacca ttattcagga acaaccctct ttcgtagcag
     4321 aaacccgtgc ggctgacaaa aatacagatg ctgttttacc gtggctggcg aaagatattg
     4381 ctgtcgtttt tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag
     4441 ttcctgttca gcaagcagac aaattgaccc aattacgtcg cttagcctgt agtgtgtcgc
     4501 aacgggataa taaaacagcc acatttgact tcagcgcctg ttctttagaa tggcaaaaca
     4561 ccgtcgccca ggcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcacccg
     4621 taatggctgt actcactgca cttgaaatga aatgcacaag atataaagag cgtgaggatg
     4681 ttatggatca gattattcag gaaggcggtc tggaatatgc tactgatgta ataatacatc
     4741 ttcaacaaat atctattgaa tgggattatg tcaacaataa tattgttttc ctgtcgtctg
     4801 gcatttcacc tgattacttg cagcaatatt ccagctttga attacgccta cgtaaacatt
     4861 tatcactggc ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc
     4921 cacatattcc agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa
     4981 ttgcacatga aatttcccag cgtctactgg ggcaaaagaa attaccctcg cttgagtggc
     5041 taaaaatagt ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat
     5101 atgccatttt tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc
     5161 aaggtgttac agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg
     5221 tgttgcgtca tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata
     5281 ctaaacgctg tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg
     5341 cagcactggc ggaacttcta gtgcaaaaag aagagaatag ttggcgcatt atgctaatga
     5401 cgatgcttat ctcgcatccg acactagcag aacaggtcat tccctggctt tcgacacccg
     5461 cagttgcagt gctgaaatca tgtcagcaac aactgacaca gccctcaaac catgccagcg
     5521 ccgatctact gccggccata gtagtctccc caccatggct ttcgaaaaag aaaaaatcgc
     5581 cgattccggt gctggattta gcaccgctca acctcgaatc catatgcaca ataaccgaca
     5641 cagaagctaa agagtttcaa actcattggg actgggaacc gcataagcca agtgaaggtg
     5701 ctaaaaattt cctttactcg ctggggtatc gccgctggga ttttgacaca tataaatata
     5761 tcggcgcttc tgactcggca attgacgcat gggaacgtga ggacttcgct acactcatcc
     5821 aaatgtttaa agcccaccat gcaccgtatc agggggagtg gcatctcaac tcacttccct
     5881 ttttacctat gcaaaaagcg attaagctat gggaatttct cagcaaagag ccgcataccg
     5941 cgataaaacc agtcatgctt tatttgcgac tggcaggtat gagtggtttt ctacactcgt
     6001 tttcacgcta ccctcaagaa ggttttgcgg ttgctaatta ttttgccgca acggaactgg
     6061 ctcctgccgt cgcccgcgcc ttcaacaaac tcaaaacatt acgacaagac gccagtagct
     6121 ggctgctgaa atacccggaa cacgccatta ccggcctgct gcctgcagcg ctcggcaaag
     6181 ccagtgaagc acaggacaac gcccgtgctg ccttgcgtat gcttaccgaa aacggtcatc
     6241 agccattgct gcaagaaatc gcccggcgtt ataaccagcc ggaagtaacc gatgcggtga
     6301 acgctctact tgcgctcgat gccttagata atcacccgac aaaaatcccc actcttccga
     6361 ccttttatca gccatcgctc tggacgcgcc cgttattaaa agcaaacgcc caatcactgc
     6421 ctgatagcgc actcctccac ctcggtgaaa tgctccgctt ccctcaggaa gaggcactgt
     6481 atccgggatt attgcaggtg aaagatgcct gtaccaccga ctcactggct gaatttgcct
     6541 gggatctgtt taccgcctgg cagaccgctg gcgcgccgtc gaaagagagt tgggcgttca
     6601 ctgcgttagg cgttctcggt aacgatgaca ccgcccgcaa actgacgcct ttgattcgcg
     6661 cctggcctgg tgaatcccag cataaacgcg ccaccgtcgg gttggatatt ctcgctgcta
     6721 tcggtagtga tatcgccctg atgcagctta acggcatcgc ccagaaactg aaattcaaag
     6781 cattacagga gcgggcaaaa gaaaaaattg ccgacattgc cgagagccgc gaactcacgg
     6841 tggcggagct tgaagatcgg ttagcaccgg atctcggtct ggatgataat ggttcgttgt
     6901 tgctggattt cggcccacgc cagttcaccg tcagctttga tgaaacctta aaaccgtttg
     6961 tgcgtgacgc ttccggcagt cgtctgaaag acctgcccaa accaaacaaa agcgatgatg
     7021 aattacaggc gaacaatgct gttaaccgct acaaattgct gaaaaaagat gcgcgtaccg
     7081 tcgccgccca gcaggtagca aggctggaat ccgccatgtg cctgcgtcgc cgctggtcac
     7141 cggaaaactt tcagctcttc ctggttgagc atccgctggt ccgccactta acccgccgtc
     7201 tgatttgggg cgtttatagc gccgaaaacc agctactggc ttgctttcgc gtagcggaag
     7261 ataacagcta cagcaccgct gacgatgatc ttttcaccct gccggaaggc gatatctcta
     7321 ttggtattcc tcacgttctg gaaatatcac cgacggatgc tgccgccttc gggcagcttt
     7381 ttgccgacta cgaactgtta ccaccgttcc gccagcttga tcgtaacagt tacgcgctga
     7441 cagaagccga gcgcaatgcc agcgaactga cacgctgggc aggcagaaaa tgcccgagtg
     7501 gtcgggtaat ggggctggca aataaaggct ggatgcgtgg tgaaccgcag gacggtggct
     7561 ggatcggctg gatgatcaaa cctctgggcc gctggtcgtt aatcatggaa atcgatgaag
     7621 gttttgcggt cggcatgtcg ccagccgaac tcagtgccga gcaactctta agcaagctgt
     7681 ggctatggga aggcaaagcg gaaagctatg gctgggggag taattcaaca caggaagcgc
     7741 agttctccgt actcgatgcc atcaccgcca gcgagctaat taacgatatt gaagccctgt
     7801 ttgaataagg aaagagcatg gacaaggaat taccgtggct ggcggataac gcccaactgg
     7861 aactgaaata taaaaaaggc aaaacaccac tcagccatcg caattggcca ggcgaaccgg
     7921 tacctgttat cactgaaaac atcattcaga cactgggtga tgaattgcta caaaaagcag
     7981 agaagaaaaa aaacattgtc tggcgttatg aaaacttttc actggagtgg cagtccgcca
     8041 tcacgcaggc catcaacttg atcggcgaac acaaaccctc aatcacggcc cggacaatgg
     8101 cggcgctagc ctgtatcgcg caaaatgata gccaacagtt gctcgacgaa atcgtccaac
     8161 acgaggggct ggaatatgca actgaggtgg tgattgcacg ccagtttatt gtacggtgtt
     8221 atgagagtga tcctctggta gtaacattgc aatatcagaa cgaggactat ggctatggtt
     8281 atcgctcaga aacctataac gaattcgatc tccgattgcg taagcatctc tctctggcag
     8341 aggaaagctg ctggcagcgt tgcgccgaca aactcattgc cgcactgcca ggaataacca
     8401 aagttcgccg cccttttatt gcgctgaacc tcccggaaaa acccgaaatc gccaacgagc
     8461 tggtaagcct tgaatgttcg caaactcatt ttcgctcaaa agagtggtta aaagttgttg
     8521 ctgatgaccc taaggcggtg aaagaactcg cacgttactg gagccaggat atatttagcg
     8581 atcgagaagc cagctacatg tcgcatgaaa accacttcgg ctacgcggcc tgcgctgccc
     8641 ttttgcgcga acaaggactg gcagccgttc cgcgcctcgc gatgtatgca cataaagaag
     8701 attgcggcag tctgctggta caaattaacc atccgcaagt catccgcacc ttgctactgg
     8761 tggctgataa aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa aacttccccc
     8821 atgcgacgct cgccgcactg gcagaactgc tggcgttaaa agccccccct gcgcgccctg
     8881 gttatcccat catcgaagac caaaagctgc ctgcacagca aaaagcacgt gatgaatact
     8941 ggcatacgct gttacaaacg ctgatggcat cgcagccaca actggcagaa gaggtgatgc
     9001 cgtggttaag tactcaagcc cgggcagtgc tgaatagtta tttatcggca ccgcccaaaa
     9061 cggttattga tagcaccgat aacagccaaa tgcctgaaat cctcgtttca ccaccgtggc
     9121 gtagtaagaa aaaaatgaca gctccacgtc ttgatttggc accgctcgaa ttaaccccgc
     9181 aagtttactg gcaaccaggc gaacaagaga ggcttgcctc cactgagtct gcccgttatt
     9241 tcagcacgga atctcttgct gaacgcatgg aacaaaaaag tggacgagtt gtattacagg
     9301 aactgggttt tggggatgat gtatggctgt ttctgaatta tatactcccc ggaaaactgg
     9361 atgctgcacg caattcactc attgttcagt ggcattacta cccagggcgg gttgaagaga
     9421 tcatgaatgg ctggagctcc ccggaagctc aattagcaga acaggcgctt cgcagcggtc
     9481 acgtagaagt gttaattaac atatgggaaa atgacagcta ctcacgttat cgtccggaaa
     9541 agagtgtctg gaacctgtat ttattggcac agttgccgcg tgagatggct ttgcctttct
     9601 ggctacgtat caatgagaaa aagcatctgt ctgcgggtga ggactatttt ctcagtatct
     9661 tcggattgga tgctctacca ggtctgctgt tggctttttc acatcgtcca aaagaaacat
     9721 ttccgttaat tttaaatttt ggcgcaacag aactggccct gcccgttgcc cgcgtctggc
     9781 gacgtttcgc ggcgcagcgt gatctggccc gccagtggat tttacaatgg ccggaacata
     9841 cggctactgc acttatccct cttgtcttca ccaaacccag cgataatagc gaagctgcat
     9901 tacttgccct acgtttactt tacgaacagg gacatggtga attgctgcaa accgcggcaa
     9961 accgctggca gcgtacagat gtatggcctg ccctggagca tttacttaaa cagagtccaa
    10021 tagagattta cccggcacgc atgccaaaag cccctgattt ctggcatccg ctaatgtggt
    10081 ccaggccgcg cctaatcatt aatcatcaac ttattaccga tgacgctctg gaaattatcg
    10141 gcgaaatgct gcgctttacc caggggggac gttttcatag cgggctggaa caactgaaaa
    10201 cgttctgcca gccgcaaacg ctggcagctt ttgcctggga tctcttcact gcgtggcaac
    10261 aagctggtgc ccccgcaaaa gacaactggg catttctggc gttaagtctc tttggtgacg
    10321 aaagcacggc acgggatcta acgacacaga tcctcgcctg gccacaagaa ggcaaatcta
    10381 cccgtgctgt cagtggcctg aacatcctta ccctgatgaa taatgatatg gcgctgatac
    10441 agctgcatca tatatcgcaa cgggcgaaat cccgcccctt acgtgataac gcggcggaat
    10501 ttcttcaggt ggtcgcagaa aatcgcgggc taagccagga agagttagcg gacagattag
    10561 tcccaacact gggccttgat gatccgcagg cgttgagttt tgattttggt ccccggcagt
    10621 ttaccgttcg cttcgatgaa aaccttaacc cggttatctt tgatcagcaa aacgttcgcc
    10681 agaaaagcgt tccccggttg cgcaccgatg acgatcaact gaaagcgccc gaggcactgg
    10741 cccgattaaa agggctaaaa aaagatacga ctcaggtgag caaaaacctg ctcccccgtc
    10801 ttgaagctgc cctgcgtacc acccgacgct ggtcgctggc agattttcat tctctgtttg
    10861 ttaatcatcc ctttacccgt ctggttaccc agcgattaat atggggcgtg tatccggcaa
    10921 atgaaccgcg tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg
    10981 cgcaagatga gcccattgac ctgcctgcgg atgctctgat tggcattgcc cacccgttag
    11041 aaatgacaga aggaatgcgc agtgaatttg cacagctttt tgccgattac gaaattatgc
    11101 cgccttttcg ccagttggcg cggcgcacgg tgctgctcac acctgacgag tcaaccagta
    11161 acagcctgac tcgctgggaa ggtaaatccg ctaccgttgg gcagcttatg ggaatgcgat
    11221 acaaaggctg ggagtcaggc tatgaggacg catttgtcta tggcctgggc gagtaccggc
    11281 tggtccttaa gttttcaccc ggttttaacc actacaatgt tgatagcaaa gcgttaatga
    11341 gcttccgttc tcttcgagtg taccgtgaca ataaatccgt cacttttgcc gaacttgatg
    11401 tgtttgattt gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa aaacacagcg
    11461 ggcagtgatg caactgcccg ttatcaacac gacttaccag cggggaaagc gatgatcgtg
    11521 caaaaagagc tggttgctat ttacgattat gaggtccctg tacctgaaga tccgttttcc
    11581 ttcagacttg agatccataa atgctctgaa ttatttacag gttccgtcta tcgactggag
    11641 cgattccggc tacgtccaac atttcatcaa cgtgatcgag aagatgctga cccgctaata
    11701 aatgatgcgt tgatttatat aagagatgag tgtattgatg agcgg
//
LOCUS       DAZGAP010000162        11714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGAP010000162 DAZGAP010000000
VERSION     DAZGAP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.163"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..641
                     /gene="ecpR"
                     /locus_tag="ACHLAM_004004"
                     /gene_synonym="matA"
     CDS             99..641
                     /gene="ecpR"
                     /locus_tag="ACHLAM_004004"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO7855459.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            715..1302
                     /gene="ecpA"
                     /locus_tag="ACHLAM_004005"
                     /gene_synonym="matB"
     CDS             715..1302
                     /gene="ecpA"
                     /locus_tag="ACHLAM_004005"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO7855460.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1359..2027
                     /gene="ecpB"
                     /locus_tag="ACHLAM_004006"
                     /gene_synonym="yagY"
     CDS             1359..2027
                     /gene="ecpB"
                     /locus_tag="ACHLAM_004006"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO7855461.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2053..4578
                     /gene="ecpC"
                     /locus_tag="ACHLAM_004007"
                     /gene_synonym="yagX"
     CDS             2053..4578
                     /gene="ecpC"
                     /locus_tag="ACHLAM_004007"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO7855462.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4568..6211
                     /gene="ecpD"
                     /locus_tag="ACHLAM_004008"
     CDS             4568..6211
                     /gene="ecpD"
                     /locus_tag="ACHLAM_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO7855463.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6180..6890
                     /gene="ecpE"
                     /locus_tag="ACHLAM_004009"
     CDS             6180..6890
                     /gene="ecpE"
                     /locus_tag="ACHLAM_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO7855464.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7203..7532
                     /gene="ykgJ"
                     /locus_tag="ACHLAM_004010"
     CDS             7203..7532
                     /gene="ykgJ"
                     /locus_tag="ACHLAM_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7855465.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7544..7710)
                     /locus_tag="ACHLAM_004011"
                     /pseudo
     CDS             complement(7544..7710)
                     /locus_tag="ACHLAM_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7781..8395)
                     /gene="yagU"
                     /locus_tag="ACHLAM_004012"
     CDS             complement(7781..8395)
                     /gene="yagU"
                     /locus_tag="ACHLAM_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO7855466.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8610..8795)
                     /locus_tag="ACHLAM_004013"
     CDS             complement(8610..8795)
                     /locus_tag="ACHLAM_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855467.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8844..9001
                     /locus_tag="ACHLAM_004014"
                     /pseudo
     CDS             8844..9001
                     /locus_tag="ACHLAM_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835868.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9016..9091)
                     /locus_tag="ACHLAM_004015"
     tRNA            complement(9016..9091)
                     /locus_tag="ACHLAM_004015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9056..9058),aa:Thr,seq:cgt)
     gene            complement(9206..10459)
                     /gene="proA"
                     /locus_tag="ACHLAM_004016"
     CDS             complement(9206..10459)
                     /gene="proA"
                     /locus_tag="ACHLAM_004016"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO7855468.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKTEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     SRGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(10471..11574)
                     /gene="proB"
                     /locus_tag="ACHLAM_004017"
     CDS             complement(10471..11574)
                     /gene="proB"
                     /locus_tag="ACHLAM_004017"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO7855469.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         3011 a         3068 c         2933 g         2702 t
ORIGIN      
        1 tttactaaag aaactgaatg tacctgtaaa aattacaggt ttggaaagta gtgacatggc
       61 aaaatgatta cagcagggac tatgaggtta aaaaccatat ggaatgtcaa aaccgttctg
      121 ataaatacat ctggtctccc catgacgcct acttctataa aggactatct gaactgattg
      181 tggatatcga cagattaatt tatctatcgc tggagaaaat cagaaaagat ttcgtgttta
      241 tcaatctcaa tacggattct ttaactgagt ttataaaccg tgataatgag tggttatccg
      301 cggtaaaggg gaaacaggtc gtattgattg cggccagaaa gtcagaagcc ttagcaaatt
      361 attggtatta caacagcaat attaggggcg tggtatacgc tggactgagt cgtgatatta
      421 gaaaagaact ggcctatgtg attaatggca ggttcctgag aaaagatatt aagaaagata
      481 aaatcactga ccgggaaatg gaaattatcc gcatgacggc tcagggaatg ctgcctaaat
      541 cgattgccag aattgaaaat tgtagtgtga agacagtgta tacccatcgg cggaatgcag
      601 aggccaagct gtactcaaaa ttatataagt tggttcagta aactccaggc aagttagttt
      661 taaaaatgac tcaccgggac ttcatgtcct caattcaact cgggaagaaa agcaatgaaa
      721 aaaaaggttc tggcaatagc tctggtaacg gtgtttaccg gtacaggtgt agcgcaggct
      781 gctgacgtaa cagctcaggc tgtagcgacc tggtcagcaa cagccaaaaa agacaccacc
      841 agtaagctgg ttgtgacgcc actcggtagc ctggcgttcc agtatgccga aggcattaaa
      901 ggttttaact cacaaaaagg tctatttgac gtggctatcg agggtgactc aacggctacc
      961 gcctttaaac tgacctcacg ccttatcacc aacaccttaa cccagttgga tacctcaggt
     1021 tccacactga atgtgggcgt ggattataac ggcgcggcag tcgaaaaaac tggcgatacc
     1081 gtgatgatcg ataccgccaa cggcgtactg ggcggcaacc ttagcccact ggctaacggt
     1141 tacaatgcca gcaatcgtac caccgcacag gatggtttca ctttctccat catcagcggc
     1201 accaccaatg gtaccaccgc agtaaccgat tacagcactc taccggaagg catctggagc
     1261 ggcgacgtta gcgtacagtt cgacgctacc tggaccagtt aatctctctg atgtaccagc
     1321 aggggtaccc ccctgcttta ttcctggatg gaattgttat gaaaaagcac cttctgcctc
     1381 tcgctctgct gttttccgga atatcaccgg cccaggcgct ggatgtcggc gatatatcat
     1441 cgtttatgaa cagtgacagc agcacgctaa gcaaaacgat ccaaaacagt accgacagtg
     1501 gccgcctcat caatatccgt ctcgaacggc tctcttcacc gcttgacgac gggcaggtta
     1561 tcgcaatgga caagccggat gagttgctac tcactcccgc cagcttgctg ctacccgccc
     1621 aagccagcga agtgatccgc ttcttctata agggaccagc agatgaaaaa gagcgctact
     1681 accgcattgt ctggtttgat caggccctca gtgatgcgca gcgcgataat gccaaccgca
     1741 gcgctgtggc cactgcttcc gcccgcatcg gcaccattct ggtcgtcgca ccccgccagg
     1801 caaactacca ctttcagtac gccaacggct ccctgacaaa tacaggaaat gcgacgctgc
     1861 ggatcctcgc ctacggcccc tgcctgaaag ccgccaatgg taaggagtgt aaagagaatt
     1921 actacctgat gccgggcaag tcgcgtcgtt ttacccgcgt ggacacggcg gataacaaag
     1981 gacgggttgc actttggcag ggtgataagt tcattcccgt gaaatagata gctgtgcaga
     2041 tggataacga caatgccttt acgacggttc tccccaggac tgaaagccca gtttgccttc
     2101 ggcatggtct ttttgttcgt tcagcccgat gccagcgctg ctgacataag tgcgcagcaa
     2161 ataggtgggg tgattattcc gcaggccttc agtcaggcgc tccaggacgg catgagcgtc
     2221 ccgctttata ttcatctcgc cggtagccag ggtcgccagg acgatcagcg aatcggcagc
     2281 gcttttatct ggttggatga tggacagtta cgcatccgaa aaatacagct ggaagagagt
     2341 gaagataacg ccagtgtcag cgaacaaact cgacagcagc tgatgactct ggccaacgcc
     2401 ccgttcaatg aggcccttac catccccctg actgacaacg cgcagttgga tctcagcttg
     2461 cgccaactgc tgctgcagct ggtggtcaag cgcgaagcgc tgggcaccgt actacgctca
     2521 cgtagcgaag acatcgggca gtccagtgtt aacaccctca gcagtaatct gagctataac
     2581 ttcggcgtct ataacaacca gttgcgtaac ggcgggagca acacctccag ctatctgtcg
     2641 ctgaataacg ttactgcgct gcgcgaacat cacgtggtgc tcgacggctc actgtacggg
     2701 attggtagcg gtcaacagga cagtgaatta tataaagcga tgtatgaacg cgattttgct
     2761 ggtcaccgat ttgccggtgg aatgctcgac acctggaact tgcagtcctt agggccgatg
     2821 accgccattt cagcagggaa gatttacggc ctttcctggg gaaaccaggc cagctccacc
     2881 atcttcgaca gcagccagtc ggccacgcca gtgatcgcct ttttaccggc ggcgggcgaa
     2941 gtacatctca cccgtgatgg gcggctacta agcgttcaga acttcaccat gggcaatcat
     3001 gaagtggata cccggggtct accgtacggt atttacgatg tggaagttga ggtaatcgtt
     3061 aacggtcgcg tgatcagcaa acgcacccag cgggtcaata agctgtttag ccggggacgt
     3121 ggcgtcggtg caccgctggc gtggcagata tggggcggta gctttcatat ggatcgctgg
     3181 tcggaaaacg ggaaaaagac gcgaccagcc aaagagagtt ggctggcagg tgcctcgacc
     3241 tccggctcac tgagtacgtt tagctgggcg gcaacgggat atggatacga taatcaggcg
     3301 gtgggtgaaa cccgtctgac gctgccgctt gggggggcga tcaacgttaa cctgcaaaac
     3361 atgctggcca gtgacagctc atggagcaac atcgccagca tcagcgccac tctacctgga
     3421 ggcttcagtt cgctgtgggt taaccaggaa aaaacccgca ttggcaatca attgcgacgt
     3481 agcgatgccg acaaccgtgc aatcggcggc acactcaacc tgaactcact gtggtcgaag
     3541 ctgggtacgt tcagcatcag ctacaatgat gaccgccgct acaacagcca ttattacacg
     3601 gcagattact atcaaagtgt ctacagcggt acctttggtt cgcttggcct gcgggccggt
     3661 attcagcgct ataacaacgg cgacagcagc gccaatacag ggaaatatat cgctctcgat
     3721 ctctcgctac cactgggcaa ctggtttagc gcagggatga cccatcaaaa cggctacacc
     3781 atggcaaacc tgtcagcacg caaacagttt gatgaaggaa ccattcgcac tgttggtgcc
     3841 aatctgtcac gagccatctc cggcgatacc ggtgatgaca aaaccctcag cggtggggcg
     3901 tatgcacagt tcgacgctcg ttacgccagc ggaacgctga acgtcaatag cgcggcggac
     3961 ggctacatca atactaattt gaccgccaac ggcagcgtcg gctggcaggg taaaaacatc
     4021 gctgccagcg ggcggaccga tggcaacgct ggggtgatat tcgacactgg cctggagaac
     4081 gatggtcaga tcagcgccaa aatcaacggg cggattttcc cgcttaacgg caagcgtaac
     4141 tatctcccgc tctctcccta tggaagatat gaggtggagt tgcagaacag caaaaactca
     4201 ctcgacagtt acgatatcgt cagcggtcgc aaaagtcatc tgactctcta tccaggcaat
     4261 gtcgctgtca ttgagccaga ggtgaagcag atggttaccg tctccggtcg tatccgtgcg
     4321 gaagacggca cactgctggc taacgcacgg attaacaacc atatcggccg aacccgaacc
     4381 gatgaaaacg gcgagtttgt catggacgtg gataagaaat accccactat cgattttcgc
     4441 tacagtggca ataaaacctg cgaagtggca ctggaactca accaggcgcg cggtgccgtc
     4501 tgggtcggtg atgtggtctg cagcggcctc tcatcgtggg cggcggtgac gcagacagga
     4561 gaagagaatg agagttaacc tactgatagc gatgataatc tttgcgctaa tctggcctgc
     4621 aaccgcgctc agagcggcag tgagcaaaac aacctgggcg gatgctccgg cacgcgagtt
     4681 tgtgtttgtc gaaaacaact cagacgacaa ctttttcgtc actcctggcg gggcgctgga
     4741 tccgcgcctg accggtgcca accgctggac cggtttaaaa tacaatggtt caggaaccat
     4801 ctatcagcaa agcctcggct acattgataa cggttacaac accggccttt ataccaactg
     4861 gaagtttgat atgtggctgg aaaattcacc agtttcatct cctttaactg gcttgcgctg
     4921 catcaactgg tacgcagggt gtaatatgac caccagtctt atcctgccgc aaaccaccga
     4981 cgccagtgga ttttatggcg cgacggtgac cagcggcggc gcgaaatgga tgcacggcat
     5041 gttgtcagac gcgttttacc agtatctgca acaaatgccc gtcggcagca gctttacaat
     5101 gaccatcaat gcctgccaga cctctgtgaa ctatgacgcc agcagcggcg cacgctgtaa
     5161 ggatcaggcc tccggcaact ggtatgttcg caacgtcacc catacgaaag cagcaaatct
     5221 gcggttgata aatacccact cgctggcgga agtatttatc aacagcgacg gagtaccgac
     5281 tctgggcgaa gggaacgccg actgccggac gcaaaccatc ggcagccgtt caggattaag
     5341 ttgtaagatg gttaactata ccctgcaaac aaacggactc agcaacacct caatccatat
     5401 attcccggcg atcgccaact cgtcgttagc ctcggccgtc ggggcgtacg atatgcagtt
     5461 cagtctgaat ggcagttcat ggaaaccggt gagcaatact gcctattact acaccttcaa
     5521 cgagatgaag agcgcagact cgatctatgt tttcttctcg agcaacttct ttaaacagat
     5581 ggtgaacctc gggatcagcg atatcaacac caaagatcta ttcaactttc gctttcagaa
     5641 caccacatca ccggagtctg gctggtatga attttctacc tccaacacgc tgattatcaa
     5701 accccgtgat ttcagcatca gtattatctc cgatgaatat actcagacac cgtcgcggga
     5761 gggatatgtt ggcagcggcg agtcggcact cgatttcggc tatatcgtaa ccaccagcgg
     5821 taaaacagct gccgacgaag tgctgatcaa ggtgaccgga cccgcgcagg tgattggcgg
     5881 gcgctcctat tgtgtcttca gctccgatga cggtaaggcg aaagtaccgt tcccggcgac
     5941 gctttccttt attacccgca acggagctac aaaaacctac gatgccgggt gcgatgatag
     6001 ctggcgggat atgaccgatg cgctgtggtt gaccacaccg tggactgata tctctggcga
     6061 agtggggcag atggataaga ccacagtcaa attttcgatt ccaatggata acgccatttc
     6121 tctgcgtacg gtagatgata acggctggtt tggcgaagtc agcgcttcag gagagattca
     6181 tgttcaggcg acgtggcgta acattaacta aggccctgct gacagtggtc tgtatgctgg
     6241 cggcaccttt gacacaggcg atttcggtcg gcaatctgac tttttcactg ccgtccgaga
     6301 ctgactttgt cagcaaacgt gtcgtgaata acaacaaaag cgcgcggata taccgtattg
     6361 ccatcagtgc tattgatagc ccgggcagca gtgaattgcg cacccgaccg gtggatggtg
     6421 aactactttt cgccccccgc cagctggcgt tgcagactgg tgagagcgag tattttaaat
     6481 tttactatca tggtccacgg gataaccgcg agcgctacta ccgggtctca tttcgcgagg
     6541 tccccactcg taacctgaca aaacgcagcc caaccggcgg tgaggtcagc acggagccgg
     6601 tggtggtgat ggataccatt ctggtagtac gaccacgtca ggttcagttt aaatggtcct
     6661 tcgatcaggt gaccggaacg gtaagtaaca ccggcaacac gtggtttaag ctactgatta
     6721 aaccaggatg tgattcgacc gaagaggaag gcgatgcctg gtatctacgt ccgggagacg
     6781 tggttcatca gcctgagtta cgtcagccgg ggaatcatta tctggtctat aacgacaaat
     6841 tcattaagat tagcgattcc tgtccggcta agcccccttc ggcggactaa gacttatcca
     6901 tcggacgagg gaagaattcc acctcagaga ctcaaaaatc gtttaaatga tgtggaagcg
     6961 atcgtcaatg tcacctgtaa cgatgagctt tctcatatgc acaccacagt gttcaggaga
     7021 cacggaaagg taccaggcgg cctgctaacg agtggtttct tcactaatta ggacgtaaaa
     7081 tcggtaacgg ctggaaatca ttcaatactc acactatcga aagtacacca gccaaccgcg
     7141 atacatccct gcatacgacg taccgcagtt ctctttacga tttatagctg tactggtgaa
     7201 ttatgagcaa tctgaatcca tgcatgacgt gtggtgcctg ttgtgcattt ttccgcgtct
     7261 ctttttactg ggccgaagct gacgatgctg gcggtaccat tcccgccagg ctcactgaac
     7321 aaatatcccc ttttcaccga tgcatgagcg gtactaatca gaaaaatccc cgatgtattg
     7381 cccttgcagg aacaccgggc aaaaatgcct gctgcacgat atatgaaaat cgatcgtcca
     7441 catgcagaga attcgccatg tctggtgaga acggagaagt caatgaggct tgcaatcgtg
     7501 caagagctaa atacgggcta acaccgttat aaatatacaa taattaattg cattgccccg
     7561 ccagctgtaa tagcggggct tcgtttttca gggacaacaa aacctgaaac ttcttccatt
     7621 ttccagcaac aatgcctctt cttctgtagc gttctccgga tctacatcat cctgaagtag
     7681 cgcgatatga tcactgactg tccgcatcag aatatgaaaa gcaacgtttt taacctataa
     7741 aaatggcgct atatttgcgc catttttatc attcaaagca ttatctattt gagcctaaag
     7801 ggatctcagg atctggctca tgggtaattc tgtttcgtaa atctctgcga ataatttcaa
     7861 tagaccagaa ccagactaaa tgtccaaaaa tttcagagac attctcatac cacggaagat
     7921 caaagagagg tggcgtaagt cccatgagag ggaatgaaat catatgaaca aaaagttgcg
     7981 ctaaagcacc tgccagtaag ccctgccaaa gtttaatttt cggaaatact tcggcgacca
     8041 cacagtaacc aacggcaaaa actatcgaaa agattatgtg cgttacacca acccagttaa
     8101 agacatgccc ggcgaaggta taaacagccg aatttggatc tgtcagtccc aaccaatccc
     8161 gaagaaaaat gtacggagga ttgagaaagt tacgggagca atcaatttgg cctgcagccc
     8221 tgattaatga ttccggacca cacgctgcat taaacatatc aaccggacta cgtggtggca
     8281 atggaacttc tgccccccac ttaacaaatg cggaaacaac gccagcaatc agcccaatga
     8341 atgcagcaag accataacgt ctgcggtttg gtggagtttg ttcaaatata ttcatatcta
     8401 ccctgcttgt accattatgt tatacacctc tttaggagta ttcataaaac aaggcaaatg
     8461 taaagaactg tattgttttg tataaccaca tggttaccta atcgccaatt aatataagct
     8521 ccattatttc tccatatttt catgttagaa gtgacaagga gaaatgtaat gtttctctca
     8581 gagaatataa atcattcctt ttgcccacct taatggtggg cttttttatg caaaacctga
     8641 acgtcctgtg gtattcctct ggcataatga ttttcgcgat taaccaacaa ccagaccagc
     8701 aaagacgcca ccacaagcac agtaacatcc atcaggcttg ccacacccca tgcgtgcggc
     8761 tcaaagccgc catggcatat taatccgctt gccattttcg aacagctgat tggtttggtc
     8821 cggtgtttcc ctgcttgcac ttaaaaatga gcggatggga aactctcgaa atggttcgtc
     8881 gatatgctca cctcgcacct aatcacctta ccgaacacgc acggtaaata gattcgatcc
     8941 tgaacccatc ggtcccaaat ctgtcccaat aaaaaataag gaaggtatta gtgatgtgta
     9001 acttattgat ttaaatggtg ccgataagag gagtcgaacc tccgaccttc gcattacgaa
     9061 tgcgctgctc taccaactga gctatatcgg ccctgaaagg acatgttcac gaacgtgaat
     9121 cacggtggac aaggttaaaa ctaaccgggc gatgcgtcaa tggccttgtg aatcaaatgg
     9181 ctacttttgc atcacccggt tttatttacg cacgaatggt gtaatcacca atgccgatcc
     9241 acttgtaagt ggtcagtgct tccagcccca ttgggccacg cgagtggagt ttttgtgtgc
     9301 ttaccgccac ttccgcaccc agtccaaact ggccgccgtc ggtaaaacgc gtagaggcgt
     9361 taacgtaaac agcggaggaa tctacttcgt taacaaaacg ctgggcgttg cgcatatcgc
     9421 gggtcaggat cgcatcggag tgttgcgtgc cgtgttcacg aatatgggcg atggcatcgt
     9481 caagatcgct gacgattttg acgttcaaat ctaatgacag aaactcatcg tcatactctt
     9541 cggttttaac agcaaccacc ttcgcggggc ctgtctgcaa ctgcgccagt gcacctgcat
     9601 cagcgtgtaa cgtcacgcca ctttccgcca tttgtttgct taatgcgggc aggaaactat
     9661 cggcgatgtt tttattcacc agcaacgttt caaccgtatt acatgtgctc ggtcgctgag
     9721 ttttcgcgtt gacgatcact ttcagggctt cagcgatctc tgcactttca tcaacgtaaa
     9781 tatggcatac gcctatacca cctgtgatca ccgggatcgt cgactgttcg cggcacagtt
     9841 tatgcaggcc agcgccaccg cgcgggatca gcatgtcgat gtatttatcc atacgcagca
     9901 tttcactgac cagcgcgcgg tcaggattat caatcgcctg cacggcaccc gccggtaagc
     9961 tgcaggattt cagggcgtcc tgaatcaccg ccaccgttgc agcgttagtg cgacacgttt
    10021 ctttgccgcc gcgcaggatc accgcgttac ctgttttcag gcacagcgaa gcgacatcta
    10081 ccgtcacgtt cgggcgcgct tcataaatca cgccaatcac ccccagcggc acgcgacggc
    10141 gctccagacg cagaccactg tccagtacgc cgccatcgat tacctgcccc accggatcgg
    10201 cgagattaca cacctgacgc acatcgtcgg cgatgccttt cagccgtgcg ggcgtcagtg
    10261 ccagacggtc aagcatcgct tcgctaaggc cattggcacg cgcgtcagca acatcctggg
    10321 cgttagcgtt gaggatgatt tcgctttgtg cttccagttc atcggcgatt ttttccagca
    10381 cacgattttt ttcgcggctg gagagttgcg ctaatttata cgaggcttgc tttgcggcaa
    10441 tgcccatttg ttccagcatc ggccagctcc ttaacgggta atcatgtcat cacggtgaac
    10501 ggcaaccggg ccatattcat atcccagtat tgcatcaatt tcttgcgagt ggtgtccggc
    10561 aatgcggcgt aatgcatcgc tgttgtaacg actgacgccg tgggcgatat cgcgaccttc
    10621 gaggttgcaa atgcggatga cttcaccacg cgagaaattg ccagttacgc tcttaatgcc
    10681 tttcggcaac agggagctgc cgcgttcaag aatggcggcg gttgcccctt catctaccgt
    10741 gatttcaccc gccggcggcg caccgaaaat ccagcgttta cggttttcaa gcggagtcgc
    10801 ctgggcatgg aacagcgtac cgacggaaat gccttccatc acatcaccaa taacgcccgg
    10861 cttgctgccc gcggcaataa tggtgtcgat acccgcacgg caagccacgt ccgcggcctg
    10921 caatttggtg ctcataccgc cagttccgag gcctgaaacg ctatcaccgg caatcgcgcg
    10981 cagtgcgtca tcaatgccgt acacatcttt aatcagttct gcctgcggat tgctgcgcgg
    11041 gtcagcggta tacaaacctt tttgatcggt cagcagcaac agtttatcgg cacccgccag
    11101 aatcgccgcc agcgcagaaa ggttatcgtt atcaccgacc ttaatctctg ccgtagcgac
    11161 agcatcgttc tcattaatta ccggaacgat attgttatcg agcaacgcac gcagggtatc
    11221 gcgggcgttc aggaagcgtt cacgatcttc catatcagca cgggtcagca acatctgccc
    11281 gacatgaatg ccgtaaattg aaaacagctg ttcccacagt tgaatcagtc gactctgccc
    11341 taccgccgcc agcagctgtt ttgaagcgat agtcgctggc aattccgggt accccaggtg
    11401 ctcacgtccg gcggcaatcg cacccgacgt cacaataaca atccgatgcc cggcggcatg
    11461 taactgcgcg cactggcgaa caagttcaac gatatgggca cggttcaggc ggcgcgatcc
    11521 gcctgttagc acactggtgc cgagttttac caccagcgtc tggctgtcac tcatgattct
    11581 ctgccattca attttaggaa aaaatgatat caaacgaacg ttttagcagg actgtcgtcg
    11641 gttgccaacc atctgcgagc aaagcatggc gttttgttgc gcgggatcag caagcgtagc
    11701 ggcagttgtt tacg
//
LOCUS       DAZGAP010000163        11591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGAP010000163 DAZGAP010000000
VERSION     DAZGAP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.164"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..977)
                     /gene="pyrD"
                     /locus_tag="ACHLAM_004018"
     CDS             complement(<1..977)
                     /gene="pyrD"
                     /locus_tag="ACHLAM_004018"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO7855470.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGP"
     gene            1214..1789
                     /gene="ssuE"
                     /locus_tag="ACHLAM_004019"
     CDS             1214..1789
                     /gene="ssuE"
                     /locus_tag="ACHLAM_004019"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO7855471.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1782..2741
                     /gene="ssuA"
                     /locus_tag="ACHLAM_004020"
     CDS             1782..2741
                     /gene="ssuA"
                     /locus_tag="ACHLAM_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO7855472.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2738..3883
                     /gene="ssuD"
                     /locus_tag="ACHLAM_004021"
     CDS             2738..3883
                     /gene="ssuD"
                     /locus_tag="ACHLAM_004021"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO7855473.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            3895..4686
                     /gene="ssuC"
                     /locus_tag="ACHLAM_004022"
     CDS             3895..4686
                     /gene="ssuC"
                     /locus_tag="ACHLAM_004022"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO7855474.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4683..5450
                     /gene="ssuB"
                     /locus_tag="ACHLAM_004023"
     CDS             4683..5450
                     /gene="ssuB"
                     /locus_tag="ACHLAM_004023"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7855475.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(5656..8268)
                     /gene="pepN"
                     /locus_tag="ACHLAM_004024"
     CDS             complement(5656..8268)
                     /gene="pepN"
                     /locus_tag="ACHLAM_004024"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO7855476.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVRWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8534..9736
                     /gene="pncB"
                     /locus_tag="ACHLAM_004025"
     CDS             8534..9736
                     /gene="pncB"
                     /locus_tag="ACHLAM_004025"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO7855477.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9905..11305
                     /gene="asnS"
                     /locus_tag="ACHLAM_004026"
     CDS             9905..11305
                     /gene="asnS"
                     /locus_tag="ACHLAM_004026"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO7855478.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2663 a         2995 c         3057 g         2876 t
ORIGIN      
        1 ggacctttaa aaataaaacc agaataaatt tgcaccagtg aggcccccgc agcaatcttt
       61 tcacgcgcag cgataaccga atcaatgccg ccaacaccga tgatcggtaa gcgaccgttt
      121 aattcctgtg acaagcggcg aataatttct gtgcttttta actgaagcgg acgaccactt
      181 aagccaccgg tttgatcaca atttttcatt ccctgaacca gagaacgatc gagcgtggta
      241 ttagttgcaa tcacgccatc aatattatgg cgaactaaac tatcggcaac ctggatcaat
      301 tcttcttcag aaagatccgg cgcgatcttc actgcgatcg gcacatattt atggtgcatc
      361 gcttgcaaat cattttgctt atttttaatc gcggttaaga gatcatccag cgcttcgcca
      421 tattgcagtg tgcgtaatcc tggggtattt ggcgatgaaa tattgatggc gatatatccc
      481 gcataggcat agattttttc catacaaatc agatagtcat ctttaccctg ctccaccggc
      541 gtatctttat ttttgccgat gttaataccc agaacgccgt cataatgggc tttttttacg
      601 ttctctacga ggttatcaac gccaagatta ttaaagccca tacggttgat caaaccttcg
      661 gcatctacca gacgaaagag acgcggcttg tcattacctg gctggggacg tggcgtgacg
      721 gtaccgatct cgatcgatcc aaatcccatc gcgcctaacg cgtcaatgca ctccccgtct
      781 ttatcaagac cggctgccag accaagcgga tttttaaacg tcaggcccat gcagttaaca
      841 ggtttcgcag gcactttctg ccgcaccagt gcttcaaacg gcgttccggt aatacggcgt
      901 aattgctgaa aagtaaactc atgagcgcgc tctggatcga gctggaaaag ggctttacga
      961 acgaaggggt agtacatgaa ctctcctgga ttcccggtgt gcaaaccggg ggcgtattat
     1021 gtgcgagttc ggaccaaaag ggaattgacc tgcggcaata aatagcaaac gttttcttat
     1081 tgcgcttttt ttatgctttt tttggctttt cttcctcaga taaatcattt cgactcgcgg
     1141 acgccctctg tcagactgac cgaattgtta tcaatgttaa caaaacaaga acaattggtt
     1201 ataaggagag agtatgcgtg tcatcaccct ggcgggtagt cctcgctttc cttctcgctc
     1261 cagctccttg ctggaatatg cgcgggaaaa actaaatggc ctggatgtag aggtttatca
     1321 ctggaatttg caaaacttcg tcccggaaga tctgctttat gctcgtttcg atagtccggc
     1381 actcaagacc ttcaccgaac agctgcaaca ggccgatggg ctgattgtcg ccacgcctgt
     1441 gtataaagcc gcctattccg gcgcgctgaa aaccctgctc gacctgctgc cagaacgtgc
     1501 gttgcaaggc aaagtggtgc tgccgctggc gacaggcggc accgtggccc atctgctggc
     1561 ggtcgattat gcccttaagc cagttttaag tacgctgaaa gcccaggaga tcctgcacgg
     1621 cgtgtttgcc gatgactcgc aagtaattga ttaccaacac aaaccccagt tcacgccaaa
     1681 tctgcaaact cgtcttgata ccgcgctaga aactttctgg caggcgttgc accgccgcga
     1741 tgttcaggtt cctgaccttc tgtctctgcg aggtaatgcc catgcgtaag atcattaaac
     1801 tggcgctggc gggtttactt agcgtctcta cacttgctgt tgccgcggag tcttcgccag
     1861 aggcgttacg tataggctat caaaaaggca gtattggtat ggtgctggca aaaagccacc
     1921 agttactgga aaaacgctat ccgcaaacaa aaatctcctg ggtggagttc cccgctggcc
     1981 cacagatgct ggaggcgtta aacgttggca gtatcgatct cggcagtacc ggggatattc
     2041 cgccaatctt tgcccaggct gccggggctg atttggtata cgtgggcgtc gagccaccga
     2101 aacccaaagc cgaagtgatt ctggtggcag aaaacagccc gatcaaaacc gtagccgatc
     2161 ttaaaggtca caaagttgcc tttcagaaag gttccagttc gcacaatctt ttactgcgag
     2221 cccttcgcca ggccgggctt aaattcacgg atattcagcc cacttacctg acgcctgccg
     2281 atgcccgcgc cgcgttccag caaggtaacg ttgacgcctg ggctatctgg gatccctact
     2341 actccgctgc attattacag ggcggcgtgc gggtgctgaa agacggcacc gatctcaatc
     2401 aaactggatc gttttatctg gcagctcgtc cgtatgcaga aaaaaacggc gcttttattc
     2461 agggtgtgct gacaaccttt agtgaggccg atgcgttaac ccgcagccag cgcgaacaaa
     2521 gcatcgcttt actggcaaaa acgatgggct taccggcacc ggttattgcc tcatatctgg
     2581 atcatcgtcc tcccaccacc atcaaaccgt tgagcgctga agttgccgcc ttacagcagc
     2641 aaacggcaga tctgttttat gaaaaccgtc tggtgccgaa aaaagtcgat attcgccagc
     2701 gcatctggca gcccactcaa ctggaaggaa aacaattatg agtctgaata tgttctggtt
     2761 tttaccgacc catggtgacg ggcactatct gggaacggaa gaaggttcac gtccggttga
     2821 tcacggttat ctgcaacaaa ttgcgcaagc ggcggatcgt ctgggttata ccggtgtgct
     2881 gatcccaaca ggacgatcgt gtgaagatgc gtggctggtt gccgcgtcga tgatcccggt
     2941 gacgcaacgg ctgaagtttc ttgtcgccct gcgtcccagc gtaacctcac ctaccgttgc
     3001 cgcccgccag gccgccacgc ttgaccgtct ctcgaatggt cgtgcgttgt ttaatctggt
     3061 cacaggcagc gatccacaag agctggcagg cgacggcgtg ttccttgatc atagcgagcg
     3121 ctacgaagcc tcggcggaat ttacccaggt ctggcggcgc ttattgctgg gggaaaccgt
     3181 caatttcaac ggtaaacata ttcatgtgcg cggagccaaa ctgctcttcc cgccgattca
     3241 acagccgtat cctccgcttt actttggcgg atcgtcagat gtcgcccagg agctggcggc
     3301 agaacaagtt gatctctacc tcacctgggg cgaaccgccg gaactggtta aagagaaaat
     3361 cgaacaagtg cgggcgaaag ctgccgcgca tggacgcaaa attcgtttcg gtattcgtct
     3421 gcatgtgatt gttcgtgaaa ccaacgacga agcgtggcag gccgccgagc ggttaatctc
     3481 gcatcttgat gatgagacta tcgccaaagc acaagccgca ttcgcccgga cggattccgt
     3541 aggacaacag cgaatggcgg cgctacataa cggcaagcgc gacaatctgg agatcagccc
     3601 caatttatgg gcgggcgttg gcttagtgcg cggcggtgcc gggacggcac tggtgggcga
     3661 tggtccgacg gtcgctgcgc gaatcaacga atacgccgcg cttggcatcg acagctttgt
     3721 gctttcgggc tacccgcatc tggaagaagc gtatcgggtt ggcgagttgc tgttcccgca
     3781 tctggatgtc gccatcccgg aaattcccca gccgcagccg ctgaatccgc aaggcgaagc
     3841 ggtggcaaat gattttatcc cccgtaacgt cgcgcaaagc taaggaatac gataatggca
     3901 acgccagtga aaaagtggtt attgcgcgtt gccccctggt ttttaccggt gggcatcgtg
     3961 gcggtgtggc aactggcctc ctcagttggc tggctgtcaa cgcgtatttt gccctcaccg
     4021 gaaggagtgg tgatggcgtt ctggacgctc tccgccagcg gcgaattgtg gcagcatctg
     4081 gcgatcagct cctggcgggc gctcattggc ttttcaattg gcggatcgct ggggttgatt
     4141 ttgggactga ttagcgggct atctcgctgg ggagaacgac tgctggatac ctccattcag
     4201 atgttgcgta acgtgccgca tctggcgctg atcccgctgg tgattttgtg gtttggcatt
     4261 gatgaatccg caaaaatctt tctggtggcg ctcggcacgc tattccccat ttatatcaac
     4321 acctggcacg gcatccgtaa tatcgatcgc ggtctggtgg aaatggcgcg tagctatgga
     4381 ttatccggca taccgctgtt tatccatgtg atcctgcctg gcgccctgcc ctcaattatg
     4441 gtcggcgtgc gttttgcttt gggcctgatg tggctgacgc tgatcgttgc cgaaactatt
     4501 tctgccaatt ccggcattgg ttatctggcg atgaatgcgc gggagtttct gcaaacggac
     4561 gtggtggtag tcgctattat tctttacgcc ctgctcggca aacttgctga cgtcagcgcg
     4621 cagctgttag agcgtctctg gttgcgctgg aacccggctt atcatttgaa ggaggccacg
     4681 gtatgaatac tgctcgtctg aaccagggca caccattgtt gctcaatgca gtaagcaaac
     4741 aatacgcgga aaatatcgtc ctgaaccaac tggatttaca tattccggca ggtcagtttg
     4801 tggcggtggt gggccgcagc ggtggtggca aaagtaccct gctgcgcctg ctggcaggtc
     4861 tggaaacgcc aaccgcaggc gacgtgttag cgggcaccac accgctggct gagattcagg
     4921 aagacacgcg aatgatgttt caggatgcgc gtctgctacc gtggaaatcg gtgattgata
     4981 acgttgggtt aggccttaaa ggccagtggc gcgatgccgc gcgtcgggcg ttggctgcag
     5041 taggactgga gaaccgcgca ggggaatggc ctgccgcact ttctggcggg cagaaacagc
     5101 gtgtggcgct ggcaagagcg ttaattcatc gaccgggttt attattgctt gatgaaccgc
     5161 tcggggcgct ggacgccctg actcggctcg agatgcagga tttgattgtg tcactttggc
     5221 aggagcacgg ctttaccgta ctgttggtga cgcatgatgt gagcgaagcg gtagcaatgg
     5281 ctgaccgggt attgttaatt gaagagggaa aaattggtct ggatttgacg gtggatatcc
     5341 cccgtccgcg ccggttaggg tcggtgcgat tagcggagct ggaagcggaa gttttgcaac
     5401 gggtgatgca gcgtgggcac tctgaacaac cgattcggcg tcatggttaa tatgccggat
     5461 gtggcacaca aaaccagcag tttcaacgga ttgtaggcct gataagacgc gtcaagcgtc
     5521 gcatcaggca tttgggcacg gctgccggat gcggcgtgaa cgccttatcc agcctacaaa
     5581 accagcattt tcaacggact tgtaggcctg ataagacgcg tcaagcgtcg catccggcat
     5641 tttggctcaa aagtatcaag ccagtgcttt agttatcttc tcgtacagat cgccagagag
     5701 attttccagc cctttcaact gctccagcgc cgcgcgcatt ttctcctgac gtttggcatc
     5761 gtaacgtttc aggcgaatca gcggctcaat caggcgtgaa gccacctgcg ggttacggct
     5821 gttgaggtcg gtaagcattt ccaccaggaa ctggtaaccg ctgccatctt cggcatggaa
     5881 cgctgccggg ttgctgcccg caaacgcacc aatcaacgaa cgaatacggt tcgggttgct
     5941 catggtaaat gagcgatgct gcaacaggcc acgcaccgtc tccagcacat tcgccgccgg
     6001 gctggtggct tgcaggataa accatttatc catcaccaga ccatcctgat gccacttgtc
     6061 gtcgtactcc tgcatcagcg cgtcacggca aggcagctgt gcagcaaccg ccgcagaaag
     6121 cgctgccagc gcatcggtca tattgtttgc ttcatggtat tgcttgctca ccagcacgtc
     6181 agccagatgc gtttcaccaa aagcaaggaa gcgcaggcag gcattacgca gagtgcgttt
     6241 tgcaatatct tcatgctcaa cacggtactc gctctggtaa ttcgcgttgt aaatagccag
     6301 tagctcatcc gccagttcag tcgccagagt acgagtgagt gcttcgcgta cttcggcaat
     6361 agcaatcggg tcgatgatat cgaacagttc agccatttca ttgacagaag gcagcgtcag
     6421 gatttctgcc gccagcgctg gatcaatctt ctcatcgagc agcaccgcgc ggaaagcgtc
     6481 agccacatgc accggcagag acagcagctg cccttgctga tgacgggcga cgtttagctt
     6541 gatgtaggtt gccagcaggc tttgcgccgc atcccaacgg gagaaatcat tacgcgcatg
     6601 acgcatcagg aaggtcagtt gctgatcgct ccacttatat tccagtttca ctggcgcaga
     6661 gaattcgcac agcagcgcag gcaccggctg gaagtagaca ttatcaaaaa caaaggtctg
     6721 ttcagcctgg gtgacgttca gcaccgaatt caccggatga ccgcctttct gcaacgggat
     6781 cactttgcct tcgttatcat acagttcgat ggcaaacgga atatgcagcg gctgtttttc
     6841 tgcctgatcc ggcgtagctg gcgtgcgctg gctgatggtc agggtgtact gctcagtttc
     6901 cggattgtag tcgtctttga cagtcacaac cggcgtgccg gattggctgt accaacggcg
     6961 gaaatgggaa agatcgacat tcgacgcatc ttccatcgcc tgcacaaagt cgtcacaggt
     7021 cgctgcgcta ccatcatgac gctcgaaata gagctgcatc cctttctgga agttttcttc
     7081 gccaagcaga gtgtggatca tacgaatcac ttccgcgccc ttctcgtaaa cggtcagggt
     7141 gtagaagttg ttcatttcaa tgaccatatc cgggcgaatt gggtgcgcca tcgggctggc
     7201 gtcttctgca aactgcaatc cgcgcatggt gcgcacatta ttgatacggt ttaccgcgcg
     7261 ggaaccaagg tcagagctga attcctgatc gcggaagacg gttaaacctt ctttcagact
     7321 gagctggaac cagtcgcgac aggtgacacg gttgccggtc cagttatgga aatattcatg
     7381 gccgataacg cgttcaatat cgaggtaatc tttgtcggtg gcggtgtcgg tgcgggccag
     7441 cacatatttg gagttaaaga tattcagccc cttattctcc attgcgccca tattgaagaa
     7501 atccaccgcg acgatcatat agatgtcgag gtcatactcc aggccgaagc gttcttcatc
     7561 ccatttcatg gagtttttca gcgaggtcat tgcccacggc gcacgatcaa ggttgccacg
     7621 atcgacatac agctccagcg ctacttcgcg accagaacgc gtggtgaaag tgtcgcgcag
     7681 cacatcaaag tcgcctgcca ccagcgcaaa caggtagcac ggtttcggga acgggtcctg
     7741 ccaccgtacc caatgacgtc cattttccag ttcaccttgc gcaacgcggt taccgttgga
     7801 aagcaggaag ggatatttgg ttttatcggc aataattttg gtggtaaaac gcgccagcac
     7861 atccgggcgg tcgagataat acgtaatatg gcggaaacct tcggcttcac actgggtgca
     7921 aagcgcatcg cctgactgat aaagcccttc cagcgcagta ttcgccgccg ggctaatttc
     7981 attaacgatc ttaagcgtaa aacgctccgg caaattactg atgaccagtg cgccctcttc
     8041 ttctttccag gcggtccacg gctcatcatt aatataaaca gaaaccagtt tgaggtcttc
     8101 gccgttgaga cgaagcggaa catctgatgc accatgacgg acagcctggc tgaccgcggt
     8161 aacgaccgtc ttttgcgcgt ccaggtcaaa ggtcaagtca atatcagtaa tctggtaatc
     8221 cggcgcacga taatcgtgac ggtatttggc ttgtggctgt tgagtcataa ataaccttta
     8281 gcatctttta tagagtctgg tgttcagtct attcctgttg cgtaaatcgc gctatgcaga
     8341 atcttcatct tttcaggtac aaacgccttt attgctacat ttttataaca tacaccgcgt
     8401 aatgccatcg accagaaagg tggcatatgg tgtgatcggg gttcaataaa ttgcgaaaca
     8461 aggtatactc cagcagttcc tgaagatgtt tattgtacta aacgctcctg tacgaggacg
     8521 ctactgcgca cctatgacac aattcgcttc tcctgttctg cactcgttgc tggatacaga
     8581 tgcttataag ttgcatatgc agcaagccgt gtttcatcac tattacgatg tgcatgtcgc
     8641 ggcggagttt cgttgccgag gtgacgatct gctgggtatt tatgccgatg ctattcgtga
     8701 acaggttcag gcgatgcagt acctgcgcct gcaggatgat gaatatcagt ggctctctgc
     8761 cctgcctttc tttaaggccg actatcttaa ctggctacgc gagttccgct ttaacccgga
     8821 acaagtcacc gtgtccaacg ataatggcaa gctggatatt cgtttaagcg gcccgtggcg
     8881 tgaagtcatc ctctgggaag ttcctttgct ggcggttatc agtgaaatgg tacatcgcta
     8941 tcgctcaccg caggccgacg ttgcgcaagc cctcgacacg ctggaaagca aattagccga
     9001 cttctcggca ttaaccgccg gtcttgatat gtcgcgcttc catctgatgg attttggcac
     9061 ccgccgacgt ttttctcgtg aagtacaaga aaccatcgtg aaacgtctgc aacaggaatc
     9121 ctggttcgtg ggcaccagca actacgatct ggcgcgtcgg ctttccctca cgccgatggg
     9181 aacacaggca cacgaatggt tccaggcgca tcagcaaatc agcccggatc tagccaacag
     9241 ccagcgagct gcacttgctg cctggctgga agagtatccc gaccaacttg gcattgcgtt
     9301 aaccgactgc atcactatgg atgctttcct gcgagatttc ggtgtcgagt tcgccagtcg
     9361 ctatcagggc ctgcgccatg actctggtga cccggttgaa tggggtgaaa aagccattgc
     9421 acattatgaa aagctgggaa ttgatccaca gagtaaaacg ctggttttct ctgacaatct
     9481 ggatttacgc aaagcggttg agctataccg ccacttctct tcccgcgtgc aattaagttt
     9541 tggtattggg actcgcctga cctgcgatat cccccaggta aaacccctga atattgtcat
     9601 taagttggta gagtgtaacg gtaaaccagt ggcgaaactt tctgacagcc caggcaaaac
     9661 tatctgccac gataaagcgt ttgttcgggc gctacgcaaa gcgttcgacc ttccgcatat
     9721 taaaaaagcc agttaatatc atcagggagc taatcggctc ccttttttta cctttaattc
     9781 cgaaatcttt cgctgcattt gcgaattctg cttgtctgat tgcagatgcc aggtaacata
     9841 ggtatccccc catttacggg tggacaagtg tttatttttt ccgactatta acagagagaa
     9901 tattatgagc gttgtgcctg tagccgacgt actccagggc cgcgtagccg ttgacagcga
     9961 agtcaccgtg cgcggatggg tacgtacccg ccgagattca aaagctggca tctccttcct
    10021 cgccgtttat gacggttcct gctttgatcc tgtacaggct gtcatcaata attctctgcc
    10081 caattacaat gaagacgtct tgcgtctgac caccggctgc tcggtcattg tgacgggtaa
    10141 agtcgtggcg tcgccgggcc aggggcaaca atttgaaatt caggccagca aggttgaagt
    10201 tgctggttgg gttgaagatc cagacactta cccgatggcg gcaaaacgcc acagcattga
    10261 gtatctgcgt gaagtcgctc acctgcgtcc gcgcacaaac ctgattggtg ccgtcgcgcg
    10321 cgttcgccat acgctggcgc aggcgctgca tcgcttcttt aacgagcagg gatttttctg
    10381 ggtttcaacg ccactgatta ccgcatctga taccgaaggt gcaggcgaaa tgttccgtgt
    10441 ttctacgctg gatctggaaa atctgccgcg taacgatcag ggcaaagtgg atttcgacaa
    10501 agacttcttt ggtaaagagt ctttcctgac cgtatctggc cagttgaacg gcgaaaccta
    10561 cgcttgcgca ttgtccaaaa tttatacctt cggcccgact ttccgtgctg aaaactccaa
    10621 caccagccgt cacctggcgg aattctggat gctggagccg gaagtggcgt ttgctaacct
    10681 gaacgatatc gcgggtctgg ctgaagccat gctgaaatat gtcttcaaag cggttctcga
    10741 agaacgcgct gacgacatga aattcttcgc tgaacgcgta gataaagatg ccgtttcacg
    10801 tctggaacgc ttcattgagg ccgatttcgc gcaggtggat tacaccgacg cagtgaccat
    10861 tctcgaaaac tgcggcagga agtttgaaaa cccggtttac tggggcgtcg atctctcttc
    10921 tgagcatgag cgttatctgg cggaagaaca ctttaaagca ccggtagtgg ttaaaaacta
    10981 tccgaaagat attaaagcgt tctatatgcg ccttaacgaa gacggtaaaa ccgttgcggc
    11041 tatggacgtt ctggctccgg gcatcggtga gatcattggt ggctcccagc gtgaagaacg
    11101 tctggacgtg ctggacgagc gtatgctgga aatgggcctg aataaagaag attactggtg
    11161 gtatcgcgat ctgcgtcgct acggtactgt tccgcattca ggtttcggtc ttggttttga
    11221 acgcctgatt gcttacgtaa ctggtgtgca aaacgtgcgt gatgtgattc cgttcccacg
    11281 tactccgcgt aacgccagct tctaatttag cgtcttcaag agccagcacc cgctggctct
    11341 ttttttatcc tttctgtcaa gttatctgtt tgttaagtca agcaatctat ttgcaacccc
    11401 gccataacga tcatcctgtt acggaatatt acattgcaac atttacgcac aaaaaataac
    11461 ccgcattctt attgcggatt agttttttct tagctgatag cacaattttc atactatttt
    11521 ttggcattct ggatgtctga aagaggattt tgtgccaggt cgataaagtt tccatcagaa
    11581 acaaaatttc c
//
LOCUS       DAZGAP010000164        11591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGAP010000164 DAZGAP010000000
VERSION     DAZGAP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.165"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..2399
                     /gene="ppsA"
                     /locus_tag="ACHLAM_004027"
     CDS             21..2399
                     /gene="ppsA"
                     /locus_tag="ACHLAM_004027"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO7855479.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2456..4102)
                     /gene="fadK"
                     /locus_tag="ACHLAM_004028"
     CDS             complement(2456..4102)
                     /gene="fadK"
                     /locus_tag="ACHLAM_004028"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO7855480.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4159..4452)
                     /gene="ydiT"
                     /locus_tag="ACHLAM_004029"
     CDS             complement(4159..4452)
                     /gene="ydiT"
                     /locus_tag="ACHLAM_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7855481.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4449..5738)
                     /gene="ydiS"
                     /locus_tag="ACHLAM_004030"
     CDS             complement(4449..5738)
                     /gene="ydiS"
                     /locus_tag="ACHLAM_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7855482.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5793..6731)
                     /gene="ydiR"
                     /locus_tag="ACHLAM_004031"
     CDS             complement(5793..6731)
                     /gene="ydiR"
                     /locus_tag="ACHLAM_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO7855483.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6751..7515)
                     /gene="ydiQ"
                     /locus_tag="ACHLAM_004032"
     CDS             complement(6751..7515)
                     /gene="ydiQ"
                     /locus_tag="ACHLAM_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO7855484.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7831..8742
                     /gene="ydiP"
                     /locus_tag="ACHLAM_004033"
     CDS             7831..8742
                     /gene="ydiP"
                     /locus_tag="ACHLAM_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7855485.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(8785..9936)
                     /gene="ydiO"
                     /locus_tag="ACHLAM_004034"
     CDS             complement(8785..9936)
                     /gene="ydiO"
                     /locus_tag="ACHLAM_004034"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO7855486.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(9950..11545)
                     /gene="ydiF"
                     /locus_tag="ACHLAM_004035"
     CDS             complement(9950..11545)
                     /gene="ydiF"
                     /locus_tag="ACHLAM_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7855487.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT         2779 a         2984 c         2920 g         2908 t
ORIGIN      
        1 tatcacaaaa ggattgttcg atgtccaaca atggctcgtc accgctggtg ctttggtata
       61 accaactcgg catgaatgat gtagacaggg ttgggggcaa aaatgcctcc ctgggtgaaa
      121 tgattactaa cctttccgga atgggtgttt ccgttccgaa tggtttcgcc acaaccgccg
      181 acgcgtttaa ccagtttctg gaccaaagcg gcgtaaacca gcgcatttat gaactgctgg
      241 ataaaacgga tattgacgat gttacccagc ttgcgaaagc gggcgcgcaa atccgccagt
      301 ggattatcga cactcccttc cagcctgagc tggaaaacgc catccgcgaa gcctatgcac
      361 agctttccgc cgatgacgaa aacgcctcgt ttgcggtgcg ttcctccgct actgcagaag
      421 atatgccgga cgcttctttt gccggtcagc aggaaacttt cctcaacgtt cagggttttg
      481 acgccgttct cgtggctgtg aaacatgtat ttgcttctct gtttaacgat cgcgccatct
      541 cttatcgtgt gcaccagggt tacgatcacc gtggcgtggc gctgtccgcc ggtgttcagc
      601 ggatggtgcg ctccgacctc gcatcttctg gcgtgatgtt ctccattgat accgaatccg
      661 gctttgacca ggtggtgttt atcacttccg catggggcct tggtgaaatg gtcgtgcagg
      721 gtgcggttaa cccggatgag ttttacgtgc acaaaccgac actggcggcg aatcgcccgg
      781 ctatcgtgcg ccgcaccatg gggtcgaaaa aaatccgcat ggtttacgcg ccgacccagg
      841 agcacggcaa gcaggttaaa atcgaggacg taccgcaaga acagcgtgac atcttctcgc
      901 tgaccaacga agaagtgcag gaactggcaa aacaggccgt acaaattgag aaacactacg
      961 gtcgcccaat ggatattgag tgggcgaaag atggccacac cggtaaactg ttcattgtgc
     1021 aggcgcgtcc ggaaaccgtg cgctcacgcg gtcaggtcat ggagcgttat acgctgcatt
     1081 cacagggtaa gattatcgcc gaaggccgtg ctatcggtca tcgcatcggt gcgggtccgg
     1141 tgaaagtcat ccatgacatc agcgaaatga accgcatcga acctggagac gtgctggtca
     1201 ctgacatgac cgacccggac tgggaaccga tcatgaagaa agcatctgcc atcgtcacca
     1261 accgtggcgg tcgtacctgt cacgcggcga tcatcgctcg tgaactcggc attccggcgg
     1321 tagtgggctg tggtgatgca acagaacgga tgaaagacgg tgagaacgtc actgtttctt
     1381 gtgccgaagg tgataccggt tacgtctatg cggagttgct ggaatttagc gtgaaaagct
     1441 ccagcgtaga aacgatgcct gatctgccgt tgaaggtgat gatgaacgtc ggtaacccgg
     1501 accgtgcttt cgacttcgcc tgcctgccga acgaaggcgt cggccttgcg cgtctggaat
     1561 ttatcatcaa ccgtatgatt ggcgtccacc cacgcgcact gcttgagttt gacgatcagg
     1621 aaccgcagtt gcaaaacgaa atccgcgaga tgatgaaagg ttttgattct ccgcgtgaat
     1681 tttacgttgg gcgtctgact gaagggatcg cgacgctggg tgccgcgttt tatccgaagc
     1741 gcgtcattgt ccgtctctct gattttaaat cgaacgaata tgccaacctg gtcggtggtg
     1801 agcgttacga gccagatgaa gagaacccga tgctcggctt ccgtggcgcg ggccgctatg
     1861 tttccgacag cttccgcgac tgcttcgcgc tggagtgtga agcagtgaaa cgtgtgcgca
     1921 acgacatggg gctgaccaac gttgagatca tgatcccgtt cgtgcgtacc gtagatcagg
     1981 cgaaagcggt ggttgaggaa ctggcgcgtc aggggctgaa acgtggtgag aacgggctga
     2041 aaatcatcat gatgtgtgaa attccgtcca acgccttgct ggcagagcag ttcctcgaat
     2101 atttcgacgg cttctcaatt ggctcaaacg acatgacaca gctggcgctc ggtctggacc
     2161 gtgactccgg agtggtgtct gaactgttcg atgagcgcaa cgatgcggtg aaagcactgc
     2221 tgtcgatggc gattcgtgcc gcgaagaaac agggcaaata tgtcgggatt tgcggtcagg
     2281 gtccgtccga ccacgaagac tttgccgcat ggttgatgga agaggggatc gatagcctgt
     2341 ctctgaaccc ggataccgtg gtgcaaacct ggttaagcct ggctgaactg aagaaataaa
     2401 ataaatcccc ggcggcgttt tgtcgccggg tttatgtgat ccccgaagat gaaacttatt
     2461 caatctcttc acagacatcc tgagttaatc gccgcataat atctttcctt aacaaaaact
     2521 tttgtatttt acctgaggct gtgcgcggta gtttttcgat taccacgata tgttcaggat
     2581 atttatattt cgcgacccgt ttacggctaa aaaatgccac tacatcttcc agcgataatg
     2641 aatgatgcgg tgctttcagc acgacataag cgcatgaacg ttcacctaag cgttcatcgg
     2701 gcattgcaac cacacaagca tcgtgaattt taggatgctg caataaaata tcttctactt
     2761 cacggctgct aatattttcg ccgccgcgga caataatatc tttcttgcgc ccggttattt
     2821 ttatatagcc agcctcatcc atgcggcaga gatcgccgct gtaataccag ccttcttcat
     2881 ccagcgcacg ggcggttaat tcaggttcat caaaataccc cataaacaca ttggggccac
     2941 gcgaggcttc ttcaccttcg caacctggcg gtaaagtctt gcgtgcgtca tcgaccactt
     3001 taatctctac acctgcggca gcgtaaccat cggtgtgtat aaagcgcgac aaaggatcat
     3061 cgagattcac caccgcatgc ggcgaacttt ctgtggaacc ataaacactt aataatttaa
     3121 tgccgcgctg ctggcattca cgcgccactt ttttggggat tgtggtaccg ccgcaaagaa
     3181 agaaacgcag cgctgaaagg tctgcgggct gtttctccag taaattcaaa agatcataga
     3241 caaacggcgt tgcgccgagc atacaggtgc aacgctgctg ctcaagcagc gcgagacacg
     3301 cagcaggagt gaaaatatct aacaacacgc tgcgagcacc aatcaaaaaa ggtgctgtta
     3361 cgccatgcag aaagcccgtt gcgtgaccaa gtggcgcagg catcataaag acatcctgcc
     3421 aggtcagatt tagccgcgcg caataagccc gctcactggc gagaatattg ttatgcgtta
     3481 gcatcacgcc ctttggcaga ccctcggttc cggaggtaaa cagcaccgcg gctaattcat
     3541 cgccgtggac cgttatcgcc gtggtcagag gagtattgtc ggcgataatc tgacttaatg
     3601 agagagcaga ggtggcggga gccagtttgt ccacgccgac aatttgttgt agttgtggaa
     3661 gctgattttg cagcggcagg attaaatcta ccggacgcgt ttgtttaaac aacgtcggtg
     3721 caaagaacat ttttgcctga cacttattga gcacccacac cagttctgct tctcgccagg
     3781 aaggcaacag cggcacggaa accgcaccgg ttttcaggca ggcaagatag ataacggtaa
     3841 attcacacca gcctggcagt tgaaatgcga tgcgatcgcc tgattcaata cccttcgcta
     3901 acatccagtt tgccagacag ctcgcggcgt gatcgagcgc gctataagtg tacgttgcac
     3961 catgattgtc gaccacggca attttgtctg gcatcgcacg agcggtctgc tgccagtaat
     4021 cggccagtga agcatcgccc cataaccctt gctgacgata cgccgcacga cgttgttcgt
     4081 tgaacgttaa tgtcactttc attttggact ctcgaaacag aacatcaggc ccgagatgcg
     4141 ggcctgtggg atgcatgatt agccgtagcg aaattcaata ccgaaggtgc ctgcgggata
     4201 ttgccactgt tcgagaatag tgttgccgca cagcacccga caggtgccgc actccagaca
     4261 accggcggaa tcgaaatgaa tgtttcctgc gtcatcctgc ttataaagtc cggcagggca
     4321 ggctttcatt aatttacgga attcattgat atcgggattt gccgccagaa tgatatgcgg
     4381 gtggccctca tcaacatgga atttatttac gcctaatttg atgtcgacgt taaccgtagc
     4441 gttctggctc atagcgcggt tgctccctta atgccatctt tcagcaagtt gatcagtcca
     4501 attttcttcg cgtgtcccat gatcattttg cgtaccggtt ggttaggttt gccgtcgatg
     4561 gtgaacatct cgttcatgat gtcggcgacc attcgtgggt attggctaaa caggcgcggg
     4621 ttttccatca gcgccgggat cttgcgaaaa tgctgcatat cacgcatgac gcagctttgt
     4681 tccagctcac gtttgtattg cgcaagactg ctggcggaga aatccgcgcg ttctttggcg
     4741 gcgatcactg ttgtggcggc agcctgagcc gatgcaatgg ctaaatccat gccgcggacc
     4801 gtaaaaccca aattcaggca gaagcctgcg gcgtcaccaa cgatcatcac gccatcgtta
     4861 accagctgcg gcaccattgc cagaccgccc tccggcacca tatgcgcgga gtattcaagc
     4921 agtttgccgc cgctaatcag cgggcgaatg gcggggtgtt gtttaaaatc ttccagcatt
     4981 tgcggcacac ttttttgcgc atgggcgata tcacccaatc cacaaaccag ccccaacgat
     5041 acggaatctt tgttggtata gaggaatccg ccgcccatca ggccgtcaga aggggaaccg
     5101 gcaaacagcc aggcggcacc ttcctcgccc gtaacattaa agcgatcgtt gatctgttct
     5161 ggtgtcaggc ctataacctc tttaacacca acagcgtaat gatgcggatc ggaagcggaa
     5221 accattccca gcgagcggcc aagcatcgag ttaacgccat cagccagaat caccacattc
     5281 gcttcgagaa tatcatcccc ggcctggacg ccagtgacct tgtttccttc acgaaccagt
     5341 gcatcgacgc gtacgcccgg gataaactgc gcgcccgcct gctcggcttg ttccatcaac
     5401 cacgggtcca gacgattacg caatacggta taagacgcgt gttgcggaac atctggttgc
     5461 tcgcggtgaa aatcgagggt aacggcgctt tcttcggtta agaaggagat tttctcgcgt
     5521 gtgaccttgc gttctaccgg cgctgatgct gcaaagcctg gaataattgc gtcaagtgtg
     5581 tgggcataaa gacgcccgcc ggtcatgttt ttacatccgg cactgtcgcc gcgttctatc
     5641 accaggacat ccagcccggc tcgcgccatg accagtgcgg caacgctacc ggcaacgccc
     5701 gcaccgacca caatggcatc aaatttgtca tccgacatgc gttatgctcc cttcccgctg
     5761 cgtttacagc gggagatgtg gttgaaaggg aatcagcgac tcaactggct aatcagggca
     5821 gggacgactt tgtagatatc gcccaccaga ccgtagtcgg catagttgaa gattggcgca
     5881 tttttatctt tattaatggc gacaatcact tttgcgccgt tgccgccaac catatgctgg
     5941 atctgcccgg agatccccag cgtcaggtag agatcggatt tcagcaacac gccggagaca
     6001 ccgatataac gttcacgctc catccagttc tcgccttcgg caattggacg tgaacagccg
     6061 acttcagcat tcagcaccgc cgccagttcg tggaccattt tcaggtcatc ctgcgctgcc
     6121 agaccacgac cgacgccaac cacacgtttt gctttgctca ggtccacgct gcttgccgct
     6181 ttcgcacggc gttcgcgaca gagaatttca tgacgcgggg caacataagg taccgtttct
     6241 gtcggacact gattagaggt atcactggtg cacggttcct gaacaccggg cgcaagggta
     6301 atgatcgcca gcgggctatt gatcttttcc tgtgcgaacg ctaacccgcc atacatccgg
     6361 tgttcggcgc aaatgtgacc atcgacaata tccaccgcag tggcatcgtt caccagcgcc
     6421 gcattcagtt gcacacttaa ccgtgcagcc agagctttac cgcgtttcgt cgcggccagc
     6481 agcaacatag aagggtgttt atctttcagc agggcagcaa tgctttcggc gtaattttca
     6541 gtacgttgca gcgcgtcgtt ttgcgcaaga acataaatgc attttggccc ataaggcata
     6601 actgtctgcg cctggtcggt attttgtaca atggcataca cctgttggcc ccattgctga
     6661 gcgccgccaa acagttcagc ataacgttca ggattatcgc taaagaccca gacgctgttt
     6721 aattgactca tcgtttcttc tccgtaggct tcagttcagg gctttcttca gatgttccgc
     6781 cagctcggca atggcctccg gcgaatcgtt atcgagaatg atgtgcttac gttctgtttg
     6841 cggcggtacg cgaatgccgg tcagttcagc aagtggtgcg ctctggctcc agccaatatc
     6901 acttgcctgc cactgattta ccggtttttt acccgcgccg aggatggctt tcatcgaagg
     6961 aatacgtggt acgttaatat cggaggtgac gcagagcacg gctggaacag agagttcaat
     7021 aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt gtattgccct gacgctgaat
     7081 ggcactcact gcattaatca ccggaagttg cagaatttct ccgaccagca agccaacctg
     7141 ctgggcataa aggtcgccgg aaccttcacc aaagatcagt aaatcgaagc cgatcttttc
     7201 aactgctgtc gccagcgcct ttgcggtatc gagaggcagt gcatgctcaa gttgtgcatc
     7261 ctgcaccata tacaggctgt gcggcccgcg ggatagcacg tctttgcgca ctttcgagtt
     7321 ctgcaacaaa gagccgccga cggtcagcgc ggctatctca tcgtcatctg ttgcaagctg
     7381 gcttgcagct tcaatggcat tgagatcgaa ctggctgatt ttggcgtcgg cattgtcgaa
     7441 attcagggta tattctggag tgacaacaat gtcctgttct tcaggcacca gcttaaagca
     7501 ggttattatt ttcatgattt ctcctgtgag tcgcatacgg aaccatctcc gatggaaggt
     7561 aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt tttcgacgga gcaagctgtt
     7621 ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa aacaaaagtg caatttgcgg
     7681 caatgttgtt attccatgac cgtacaatct tgcattcagt gtaaataatc ttgtttagct
     7741 ctcgctaaac gcggttgtat aaccgcaata cacgccgtat tttatccggc atatattgtt
     7801 tttgcttgct catggaatag ggaggttgtc atgtatcaac gctgttttga taacgccagc
     7861 gaaacgctgt ttgtcgccgg taaaacgcca cggctttcac gttttgcatt tagcgatgat
     7921 ccaaaatggg agtccggaca tcacgttcat gacaatgaaa ccgagttgat ttacgtcaag
     7981 aaaggggtag caaggtttac catcgattct tcgttatatg tcgcgcatgc cgatgacatt
     8041 gtggtgatag aacgtggcag gttgcatgcg gtggcctctg acgttaacga tccagcaacg
     8101 acgtgtacct gtgcgctgta cggctttcag tttcaggggg ctgaggaaaa tcagctactg
     8161 caaccgcatt cttgcccggt aattgccgca gggcagggaa aagaagtcat taaaacctta
     8221 tttaatgagc taagtgtgat tttgccgcaa agtaaaaata gccaaacatc ttcgttatgg
     8281 gacgcatttg cctatacgtt agcaattctt tactatgaaa actttaaaaa tgcttatcgt
     8341 tcggagcagg ggtatattaa aaaagatgtt ctgataaaag atattctttt ctatctgaat
     8401 aataattatc gcgaaaaaat cactttagag cagttatcga aaaagtttcg tgccagcgtc
     8461 agttatattt gccatgaatt taccaaagag tatcgcattt cccctattaa ctatgttatt
     8521 caacggcgta tgacggaagc gaaatggtca ctcactaata ctgaattatc acaggcagag
     8581 atctcctggc gtgtgggtta tgaaaatgtc gatcactttg ccaaactgtt tttgcgccat
     8641 gtcggctgtt cgcccagcga ttaccgcagg caatttaaaa actgttttgc ggaacaagaa
     8701 atcctatctg aatttcctca accagtaagt cttgccggat aaatctggcg gcgcagtttg
     8761 ttaagaagct gcgccgcctg cagattattt gttctgataa tctttcagga tctgccgacc
     8821 tgctacgtaa atcataattt cgtctgtacc gccgccgata cgttcacaac ggacatcacg
     8881 ccagaagcgg gagacgcgcg cctcatcggt atagcccaga ccgcccatga tttgaatcgc
     8941 atcatcaatg acttccattg cggtacgtgc gcagtacagt tttgccagcg ccgcgctggt
     9001 gcgcagtgac tgatgctgat cggcttgcca tgccactttc aacaccatgt tgcgcatgtt
     9061 gtcgatctta atcgccatca gcgccagttt ttcctggatc atctggttat gaccaatggg
     9121 cttaccaaaa gcgatacgct ggttggcata gcgggcggca tcttcgaatg cgcattcggc
     9181 aaagccggtg ctgcgcgcgg cgttgatcag gcgctccatc tcaaagttgt acatcacatt
     9241 gaggaaaccc attccttctt cgcccaccat gtcgctctct tcaacttcca cgttgtcgag
     9301 atagacttcg caggtgctga gcatatgcca gccgatttta tgcagtggat tgattttaat
     9361 gccgggctta ctggagtcga cccaccacag agtaaaggcc tttttcggat ctttcggttg
     9421 cggatcgcgc gccaacacca gcatatatgg atactctttc gcaccggtaa taaaggtttt
     9481 ctgtccgttg atgtaaacct tgccgttttt acgcgtgtaa gtggtagtgg cactgttgtt
     9541 gtctgagcct gcgcctggct ctgtcaatgc cagggcatag gcgggatcac ccgtttccag
     9601 ggtgctttcc gccgttttac gtagctgctc tgcagaaccg aaacggcgca tactgtgaat
     9661 acattgaccg ttggtgatca aaaatgccgg ggcaccgcat tttgacactt ccatcagcgc
     9721 cagcatttgg gtgacgtaat ccgcagggat accaccaaat tcttccggca cgccaagcat
     9781 ggaaataccg ttatccgcca gtgcccgcat aaattcacgc gggtatgtcc cgttttgatc
     9841 gcaggtgcgg aaatactctt ccggaaagtt agtcgtaatc agttcgcgaa tactggccag
     9901 cagcagttct tgttcttcag ttaaagaaaa atccatcatt atactcctgt taatgagccg
     9961 cttcaggcag gacaaaaccc atcgccgcat cgataaataa tctttcgtcc atcagtttga
    10021 gttctggcga aatcactggg gtgaaatcca ttttgtcgag aatatctttt tgtaaatcga
    10081 cgccaggagc gatttcgatt aaatgcaggc cgtcttcttt cagcgtgaat acggcgcgct
    10141 cagtgatata acgaacatcc agccctcgct cgagcgcgat ttttccgcta aaagtaattt
    10201 ctggtagttc ccgaataaat ttcttcaccc gtccttcctg gacgatattt aatttgccgt
    10261 cggtaatttc tgtttttaaa ctgcccgcag ttaatgtgcc gcagaaaacg attttcttcg
    10321 aagtggcact gatatcaata aatccaccgg tgcccatgat tttaccattg aatttatgca
    10381 cgccgacgtt accgtgctgg tcgacttcag caaaactcaa ataacaaaca tccagaccac
    10441 caccgtgata aaaatcaaac tgggacgtca tatccagaat ggcgcgggta ttcacgttcg
    10501 cgccaaaggc gatcccctgt gaagtaatac cgccaatcgg acctgtttct accgtcagaa
    10561 taaagtcatc agcacaacct tcttcccggg cgaccaggcc aatgccgtca gcaataccga
    10621 cgccgacatt ccccactgcg cctttgcgca tttcgaataa tgcacgccgt gcaactaatt
    10681 tacgttggtt taggggcagg ctaagtttgg tgctgtcatc aagggtgaag tcaccagaaa
    10741 taaagcggtt aaccggcgct ccgccataca gttgggtttg atccggatcg accaccacaa
    10801 tatccaccag ataacccgga atacggacag atttaggatg cagcgtggct ttcttaacca
    10861 ttttctgcac ctgcatcatc acaataccgc cgttattgtg caccgcctga gcaataacca
    10921 atgcgtcgag atacatcacc tcatcttcaa aagtggcgta gccttcgctg tcgcaggtgg
    10981 tagcgcgaat gaaggcgata tctggcgcta tcgctttgta atagagatat tctttgttat
    11041 caaactcgac cagtttaatc aggtcttctt tagtgacttc attcagtttg ccgccttgct
    11101 ggcgtggatc gacaaatgtt ccgatgccaa tatcactaat aataccaggc tggtgggctg
    11161 cggcggcgcg taaggtttgt gtaagtacac cttgtggata gttataagca ataattttat
    11221 tttgttctgc gagatcagaa atacgcggcg attgtcccca gtgaccgcat aatgcccatt
    11281 tcaccaggcc ttcttgcgcc agcggactaa taccacggtc ggcgcgatcg ccaagccctg
    11341 ttggactaat aatcgataaa ttacgtggtg tttgagtctg tttatattta tcagcaagag
    11401 cagtaattaa cgtggtggct tccagaatac cgccgccagc gcctaacaca caaagtgttg
    11461 cttcgtcggg aatataattc accgcttcct gtgccgacag gaccggcact cgtccattaa
    11521 tgcgaggtgg ttttacaggt ttcattgtta attcctgatt caggtgttat tttccctacg
    11581 ccagatggag a
//
LOCUS       DAZGAP010000165        11465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGAP010000165 DAZGAP010000000
VERSION     DAZGAP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.166"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..891
                     /gene="gspC"
                     /locus_tag="ACHLAM_004036"
     CDS             76..891
                     /gene="gspC"
                     /locus_tag="ACHLAM_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7855488.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVK
                     NGQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRHERTQ"
     gene            875..2827
                     /gene="gspD"
                     /locus_tag="ACHLAM_004037"
     CDS             875..2827
                     /gene="gspD"
                     /locus_tag="ACHLAM_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO7855489.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            2837..4318
                     /gene="gspE"
                     /locus_tag="ACHLAM_004038"
     CDS             2837..4318
                     /gene="gspE"
                     /locus_tag="ACHLAM_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7855490.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            4315..5511
                     /gene="gspF"
                     /locus_tag="ACHLAM_004039"
     CDS             4315..5511
                     /gene="gspF"
                     /locus_tag="ACHLAM_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7855491.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5521..5958
                     /gene="gspG"
                     /locus_tag="ACHLAM_004040"
     CDS             5521..5958
                     /gene="gspG"
                     /locus_tag="ACHLAM_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7855492.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5966..6475
                     /gene="gspH"
                     /locus_tag="ACHLAM_004041"
     CDS             5966..6475
                     /gene="gspH"
                     /locus_tag="ACHLAM_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7855493.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6472..6849
                     /gene="gspI"
                     /locus_tag="ACHLAM_004042"
     CDS             6472..6849
                     /gene="gspI"
                     /locus_tag="ACHLAM_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7855494.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            6842..7429
                     /gene="gspJ"
                     /locus_tag="ACHLAM_004043"
     CDS             6842..7429
                     /gene="gspJ"
                     /locus_tag="ACHLAM_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7855495.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKNQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7422..8405
                     /gene="gspK"
                     /locus_tag="ACHLAM_004044"
     CDS             7422..8405
                     /gene="gspK"
                     /locus_tag="ACHLAM_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7855496.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQFWPQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8420..9583
                     /gene="gspL"
                     /locus_tag="ACHLAM_004045"
     CDS             8420..9583
                     /gene="gspL"
                     /locus_tag="ACHLAM_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7855497.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMRCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            9580..10041
                     /gene="gspM"
                     /locus_tag="ACHLAM_004046"
     CDS             9580..10041
                     /gene="gspM"
                     /locus_tag="ACHLAM_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO7855498.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            10041..10718
                     /gene="gspO"
                     /locus_tag="ACHLAM_004047"
     CDS             10041..10718
                     /gene="gspO"
                     /locus_tag="ACHLAM_004047"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO7855499.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10747..11223)
                     /gene="bfr"
                     /locus_tag="ACHLAM_004048"
     CDS             complement(10747..11223)
                     /gene="bfr"
                     /locus_tag="ACHLAM_004048"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO7855500.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(11295..>11465)
                     /gene="bfd"
                     /locus_tag="ACHLAM_004049"
     CDS             complement(11295..>11465)
                     /gene="bfd"
                     /locus_tag="ACHLAM_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO7855501.1"
                     /translation="ISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAAREVMED
                     ELMQLPEFKESA"
BASE COUNT         2990 a         2947 c         2737 g         2791 t
ORIGIN      
        1 tgcaagcggc attaatacta tttatactaa cgtcaatata caacccaccc aatctatttt
       61 attagaacat acatcgtgcc cacactacgc ttccccttcc atctggcgaa tcataataaa
      121 gatgctgcca tcaatatatt gattatattt ataagcatag ggttgattat ttttaatgtg
      181 aattatttcc atacaactat agttaaaaat ggacagatca ttaatcaacc aacaaacgca
      241 tttcagtctg agttcagcct tgccgcttta tggcgtaacg aaaatcatgc tggggtaaaa
      301 gatgcaaatc ccgtggcagt taatcaggaa acacctaagc tctctatagc gcttaatggc
      361 attgtactca catcgaatga tgagacttct tttgtcttaa ttaatgaggg gagcgaacaa
      421 aagcgatact ccctcaacga agcccttgag tcagcgccgg gaacgtttat tagaaaaata
      481 aataaaacca gcgttgtgtt tgaaactcac gggcattacg aaaaagtaac tctccatcca
      541 gggctacctg acatcatcaa gcagcctgat tcagaaagtc aaaatgtact cgctgattat
      601 attatcgcca cgccgatacg tgatggagaa cagatatacg ggcttcgatt gaaccctcgt
      661 aagggcctca atgcatttac caccagcttg ctacaaccag gggatatcgc cctcaggatc
      721 aataatcttt cgctgaccca ccctgatgag gtttcacagg cattaagttt actgctaact
      781 caacaaagtg ctcagtttac aattcgtcgc aacggcgtac cccgcttgat aaatgtttcc
      841 gtcgcggaac ttacaggaat gaatggacag cgtcatgaaa ggactcaata aaatcacctg
      901 ctgcttgctg gcagcactac tcatgccttg tgcagggcac gctgagaacg aacaatacgg
      961 cgctaacttc aataacgccg atatccgcca gttcgtggaa atagtgggtc agcatcttgg
     1021 caaaacgatc ctgatcgacc cttcggtaca gggaaccatt tccgtacgca gtaatgatac
     1081 gtttagccaa caggagtact accagttctt tttaagtatt cttgatcttt acggttattc
     1141 cgtgatcacg ctggacaatg gttttctgaa agtggtgcgc tcagctaatg taaaaacatc
     1201 gccagggatg attgctgaca gttctcgtcc aggcgtaggt gatgagttgg tcacccgaat
     1261 cgtaccgctt gagaacgttc ctgctcgtga cctggctccc ctgctccgcc agatgatgga
     1321 tgcgggtagc gtcggtaatg ttgtgcatta tgaaccctcc aacgttctta ttctgaccgg
     1381 tcgtgcctcc accattaata aactgattga agtcataaag cgcgttgatg tcatcggcac
     1441 agagaagcag caaattattc atctggaata tgcgtcagcg gaagatctcg ccgaaatttt
     1501 gaatcaatta atcagcgaaa gccacggtaa aagccagatg cctgccctcc tttccgcgaa
     1561 gattgtggcg gataagcgaa ccaactctct tatcatcagc gggccggaaa aagcacgcca
     1621 gcgcatcact tcattactga aaagccttga tgtagaagag agcgaggaag gaaatactcg
     1681 ggtttattac ctgaaatatg ctaaagccac gaatctggtg gaagtgctaa ccggtgtttc
     1741 cgaaaagctg aaagatgaaa aagggaatga gcgtaagccc tcctcttctg gcgcgatgga
     1801 taacgtcgcc attaccgccg atgaacagac taactctctg gtcattaccg ctgaccagtc
     1861 cgtccaggaa aaactcgcca cggtaattgc gcgtctggac attcgccgtg cacaggtgct
     1921 ggttgaggca atcatcgttg aagttcagga tggaaatgga cttaacctcg gcgtgcaatg
     1981 ggcgaataaa aacgttggag cacagcaatt taccaatacc ggattaccga tttttaacgc
     2041 tgcgcaaggt gtggctgatt ataaaaagaa tggtgggatc accagcgcga atcctgcctg
     2101 ggatatgttt agcgcctaca atggcatggc cgcaggcttt ttcaatggcg actggggcgt
     2161 attgttgact gcgctggcca gtaacaataa aaatgacatc ctcgccaccc cgagcatcgt
     2221 aacgctggat aataaactcg cgtccttcaa cgttggtcag gatgtgccgg tgctatctgg
     2281 ctctcagacc acttctgggg ataacgtctt taatactgtc gaacgcaaaa cggtgggcac
     2341 aaaactcaaa gtcactccgc aggtcaatga aggcgacgcg gtactgctcg aaatagagca
     2401 ggaggtttcc agcgttgact cttcgtctaa ttcgacgctc ggcccgacat ttaacacccg
     2461 aactattcaa aatgccgtgc tggtcaaaac cggtgaaacg gtggtcctgg gcggattgct
     2521 ggatgatttt tctaaagagc aagtgtcaaa ggttcctctg cttggcgata ttcctttagt
     2581 ggggcaactc ttccgctata cttcgaccga gcgcgctaaa cgcaacctga tggtatttat
     2641 ccgcccaacg attatccgtg acgatgatgt ttatcgctca ctgtcaaaag agaaatacac
     2701 ccgctaccgt caggagcaac aacagcgaat cgacgggaaa tcaaaagcgc tggttggctc
     2761 ggaagatttg ccggtgctgg atgagaacac gttcaacagc catacccctg cgccctcggc
     2821 acggtaaggc attcttatga gaattcactc accgtacccc gccagttggg cgctggcaca
     2881 acgaattggt tatctctatt cagagggcga gattatttat ctcgccgata cgccattcga
     2941 acggttactc gatattcaac gtcaggttgg ccagtgccag accatgacca gcttgtcaca
     3001 ggctgatttt gaagcccggc tggaagcggt gttccatcag aataccggtg agtcgcaaca
     3061 gattgcgcag gatatcgatc aatccgtcga tcttctctcg ctttcggaag agatgcccgc
     3121 aaatgaagat ctcctgaatg aagattcagc ggcaccggtt atccgcttga tcaatgcgat
     3181 tttgagtgag gccatcaaag aaaccgcctc cgatatccac attgaaacct atgaaaaaac
     3241 aatgtcgatc cgttttcgca tcgacggtgt tttgcggaca attttacagc caaacaaaaa
     3301 actggcggca ctgcttatct cccgaattaa ggtcatggct cgtcttgata tcgccgaaaa
     3361 acgtattcca caggatggac gtattagttt gcgtatcggg cgacgtaaca tagatgtccg
     3421 cgtatccaca ctgccgtcca tctatggtga acgcgccgtg ctccgtctgc tggataaaaa
     3481 cagcctccag ctttcattga acaacctggg gatgacggca gcggataaac aggatttaga
     3541 aaatctcatc cagcttccac acggtattat cctggtgacc ggaccgacag gctccggtaa
     3601 aagcaccacg ctctacgcca tcctttcggc gctgaatact cccggccgca atattctgac
     3661 ggtagaagat cccgtggaat atgagctgga aggcattggg caaacgcagg tgaatacccg
     3721 tgtggatatg tctttcgctc gcggcctgcg cgccatactt cgccaggacc cggatgtcgt
     3781 catggtgggg gaaattcgtg atacagaaac cgcgcagatt gcggttcagg cctcgctcac
     3841 cggccatctg gtactctcaa cactccacac taacagtgca tcaggcgcag tgacccggct
     3901 ccgcgacatg ggcgtcgaat cattcctgct ttcgtcttcc ctggcaggga ttatcgcgca
     3961 acgtctggtt cgtcgcctgt gtccgcaatg ccgacaattc acgcccgtat caccccaaca
     4021 agcgcagatg tttaaatatc atcagctcgc ggtgacaaca attggcactc ccgtaggctg
     4081 ccctcattgc catcaatccg gctatcaggg gcgcatggcg atccacgaaa tgatggtggt
     4141 gacgccggaa ttacgggccg ctattcatga aaatgtggat gaacaagcac tggagcgact
     4201 ggtccggcaa caacacaatg ccttaatcaa aaatggcctg caaaaagtga taagcggtga
     4261 cacctcctgg gatgaggtta tgcgcgtcgc cagtgccacg ctggagaacg aagcatgaat
     4321 tatcgctatc gcgccatgac ccaggatggt caaaaattgc aggggatcat tgatgctaac
     4381 gatgaacgtc aggcacgact gcggctgcgt gaagaagggc ttttcctgct ggatattcgc
     4441 ccccaaaaaa gttcgggagt aaaaacacgt cgcccgagga tcagccatag tgaactgacg
     4501 cttttcaccc ggcagttggc aaccttaagc gcagcggcat tacctctgga agagagcctt
     4561 gccgtaatcg gtcaacaaag cagtaataaa cgactggctg atgtgttaaa tcaggtacgc
     4621 agcgccatcc ttgaagggca tcccctttcc gatgcgttac aacattttcc cacgcttttc
     4681 gattcgctct accgtaccct ggttaaagcg ggcgaaaaga gcgggctgct ggccccggtg
     4741 ttggaaaaac tggctgatta caatgaaaat cggcagaaaa tccgcagcaa actcattcag
     4801 tcgctgatct acccctgcat gctgactgcg gtggcgattg tggtcgtgat tattctcctc
     4861 actgctgtcg tgcccaaaat taccgaacag ttcgtgcata tgaagcagca actgccgctg
     4921 agtacacgca ttcttttagg tctgagcgac acgttgcaac ataccggccc gacattacta
     4981 gcgacagtgt ttattgtcgc tgtaggtttc tggctctggt taaaacgcgg caataaccgc
     5041 caccgttttc atgccatgtt gctgcgcgtt gcactcatcg gcccgctgat ttgcgccatt
     5101 aacagcgcac gctatctccg cactttaagt attttgcaat ccagcggcgt ccctctgctg
     5161 gatgggatga atttgtccac cgaaagtctc agcaacctcg aaatccgcaa gcgtctggca
     5221 aatgcggcag aaaacgttcg gcagggtaac agcattcatc tttcgctgga acaaacggca
     5281 atcttcccgc cgatgatgct ctacatggtg gcctctggcg aaaaaagcgg gcagctcggc
     5341 acgttaatgg tcagagcagc agataaccag gagacactcc aacaaaatcg gatcgcctta
     5401 acgctctcca tcttcgagcc agcactcatt attacgatgg cactgatcgt cctgtttatt
     5461 gtcgtctcgg tactccaacc tcttcttcaa cttaactcaa tgattaatta aggaaagtat
     5521 atgcgcgcaa cggataagca acgcggtttt acattactgg aaattatggt ggtgatcgtc
     5581 atcattggcg tacttgccag cctggtggtt cccaacctga tgggcaataa agaaaaggcg
     5641 gataaacaaa aagccgtcag cgatattgtg gcgctggaaa acgccctgga tatgtacaag
     5701 ctcgacaatc atcgctatcc aaccacaaat cagggacttg aatccttagt cgaagcgccg
     5761 acactgccac cgctggccgc aaactataac aaggaaggtt atatcaagcg tctgcctgcc
     5821 gatccctggg gcaatgatta tgtcctcgtt aatcctggtg aacatggtgc atacgatctg
     5881 ctttcagcag ggcccgatgg tgaaatggga accgaggacg acatcaccaa ctggggtttg
     5941 agcaagaaga aaaagtaagt cagcgatgaa tcagcaacgc gggtttaccc tgctggagat
     6001 gatgctggtg ctggcgctgg tcgctatcac ggcaagtgtg gtgctcttca catatggtcg
     6061 ggaggatgcc gccaacactc gtgcgcgcga aacagcggcg cggtttaccg ctgcacttga
     6121 attagccatc gaccgcgcta cgctcagtgg tcagcctgtc ggtatccatt tctctgattc
     6181 agcatggcgc attatggtac cgggcaaaac accatcagcc tggcgctggg ttccattaca
     6241 agaagatgcc gcagacgaga gcaaaaatga ctgggatgaa gaactctcaa ttcatcttca
     6301 gccatttaaa cctgatgaca gcaaccaacc gcaggtggtg attctggccg acggacaaat
     6361 cacgcccttc tctttgttga tggctaacgc agggaccggt gagccgctcc tgacgttagt
     6421 ctgttcagga tcatggcccc tggatcaaac gctcgccaga gatacccgcc catgaacaaa
     6481 caatcaggga tgacactgct tgaagtctta ctggcgatga gtattttcgc cactgttgcg
     6541 ctggctttaa tgagcagcat gcaagggcaa cgaaatgcca tcgagcggat gcgtaatgag
     6601 acgctggcgc tgtggatcgc agataaccag cttaagtcgc aagatacatt caacgatgaa
     6661 aacacgtcca gttcaggcaa agagataatt aatggcgaag agtggaactg gcgcagcgat
     6721 gtccattcaa gcaaagacgg cacgcttctg gaacgcacca taacggtgac gttacccaac
     6781 ggtcagaaaa ccgctctgac tcgttatcag agcatccatg atagatccgg gcaggcgcaa
     6841 gatgattaat cgccaacagg gtttcacatt gctggaagtc atggcggcac tggcgatctt
     6901 ctcaatgctt ggcgtgctgg cgtttatggt tttttcgcaa gcgtctacgc tacatcaacg
     6961 ctcacagaaa gaaatccagc agttcaatca actgcaacgc accatcacca ttcttgataa
     7021 cgatctcctg caactggtcg ctcgccgaaa tcggagtacg gataaaatca tggtgttggg
     7081 tgaagaggct atttttacca cgcaaagtcg cgatccgcag gccccgctca atgaagcgca
     7141 aaccttacaa acggttcact ggtatctgcg aaatcatacg ctttaccgtg ctgttcgtac
     7201 ttctgtggat ggcaggaaga atcaacctgc ccaggcgatg ctggagcatg tcgagagctt
     7261 tcttctggaa agtaacagcg gggaaagcca ggagcttcct ctctcagtca cgttacatct
     7321 gaagactcag caatatgggg cgttacagcg ccgttttgca ttacctgagc agttagcgag
     7381 agaagagtcg ccagctcaga cgcaggccgg gaataataac catgaataat gaacaacgcg
     7441 gcgtagcact gctcattgta ctcatgttac tggcgctcat ggctgctctg gctgcagaca
     7501 tgacgatcag ttttcacggc cagcttcatc gaacccgcca ggtaaaccat catttgcaac
     7561 ggcaatatga tattgagctg gcggagaaac ttgcgcttgc cagcctcacc ctggacgtta
     7621 aagataacga cagacaaacc acgttgcagc agttctggcc acaaccgcaa cagttgcagc
     7681 tggaaaatgg caatacagtg aagtggcagt tacgcgatgc ccagcactgt tttaatctca
     7741 atgccttagc aaaaatctct gacgccccgc tggcttcccc tgatttcccc gcccaggtat
     7801 tcagtgccct gctgattaac gccggtatcg atcgcggaaa caccgatgag atcgtgcaat
     7861 caattgctga ctatattgat gtcgatgact caccacgctt tcacggcgca gaagataact
     7921 tttaccaaag ccagacgccg ccccggcata gtgccaatca gatgcttttt ctgaccggcg
     7981 aattgcgtca gataaaaggc ataaccgaaa acatctatca gcggcttatc ccgtatgtct
     8041 gcgttctccc aaccacggaa cttagcatca atcttaatat gcttacggag aacgatattc
     8101 cgctcttcag agcgttattc ctcaataaca tcacggatgc tgacgctcgg gtcttattac
     8161 aaaaaaggcc cagggaaggc tggctaacca cggatgcatt tctttactgg gcgcagcagg
     8221 atttctccgg cgtgaagcct ctggtagctc aggtgaaagg gcatctcttt ccctatagcc
     8281 gctattttac gctgagtacg gaaagtatca gcgacgaaca atcccaggga tggcaaagcc
     8341 atattttctt caaccgtaaa cagcagagcg cccaaatcta tcggcgtacg ctgcaactct
     8401 actaatcata aggatcgtga tgcctgaatc gttgatggtc atacgttctt cctcgacgct
     8461 gcgtaagcat tgggaatgga tgacgttctc cgcagatagc gtatcttcgg tacatacgcc
     8521 cacggacgat cttccgctgg agtcgctggc cgatcagccc ggtgccggaa atgttcatct
     8581 tttgatccca ccagagggcc ttttatatcg ttccctcacg ctgcccaacg ccaaatacaa
     8641 actcaccgca caaacattgc aatggctggc agaggagacg ttacccgaca acacccagga
     8701 ctggcactgg acggttgtcg ataaacaaaa cgaaagtgtc gaggtgatag gcattcagtc
     8761 ggagaaactc agtcgctatc ttgagcgttt acatactgcc ggactcaacg tgacgcgggt
     8821 tttgccagat ggctgttatc ttccctggga agtagatagc tggactctgg ttaaccagca
     8881 aacaagctgg cttattcgca gtgccgcaca tgccttcaat gaactggatg aacactggtt
     8941 acagcatctt gctgcccagt ttccgcctga gaatatgcgt tgttatggtg tggcacctca
     9001 cggcgttgca gcgaccaatc cacttatcca gcatcctgaa ataccatcac tgagtttgta
     9061 ttctgccgat attgcattcc agcgctatga catgctgcat ggtgtttttc gtaaacagaa
     9121 aactgtaagc aaatcgggaa aatggctcgc ccgcctggca gtaagctgtc tcgttctcgc
     9181 cattctttct tttgttggca gccggggcat cgcgctctgg caaacgctta agattgaaga
     9241 tcaacttcag catcaacagc aagaaacctg gcaacgttat ttcccgcaga tcaaacgcac
     9301 ccacaacttt cgtttttact ttaagcagca actcgctcag caatatcctg aagctgtgcc
     9361 gttgctctat catttacaaa cacttctgct ggaacaccct gaactgcagt taatggaggc
     9421 gaactatagc cagaagcaaa agtcgctgac cttaaaaatg agtgctaaga gtgaagccaa
     9481 tatcgatcgt ttctgtgaat taacacagtc ctggcttccg atgcaaaaaa cagaaaaaga
     9541 tccggtcagt ggtgtatgga cagtaaggag ctcagggaaa tgattaaatc atggtgggca
     9601 gaaaaatcca catcggaaaa acagctcgtt gcggcgctgg cggttctctc gctcggcgtt
     9661 ttttgttggc ttggggtgat aaaaccgata gatacctaca ttgcagagca tcagtcccac
     9721 gcgcaaaaaa ttaagaagga catcaaatgg atgcaggatc aagcgagcac ccatggttta
     9781 ctcggtcatc ctgcgcttac gcagcccatc aaaaatatcc ttctcgagga agcaaaacgt
     9841 gagaatttgg ctatcacgct ggaaaacggt ccggacaata ctctgacaat tcatcctgtc
     9901 acagctcccc tcgaaaacgt ctcccgctgg ctcactacgg cacagattac atacggtatt
     9961 gttattgaag accttcagtt tactcttgcc ggaaacgaac agatcaccct caagcatttg
    10021 tccttcaggg agcaaccata atgacaatgc tactgccgct cttcattctg gttggtttta
    10081 ttgcaggtta ttttgttaat gccatggcct atcacctctc gccgttggag gataaaacgg
    10141 cgttaacgtt tcgccaggta ttggttcatt tcaggcaaaa aaaatatgcc tggcatgata
    10201 cagtgcccct gatactttgc gttgctgctg ctatcgcctg cgccctggca cctttcacgc
    10261 ccatcgtgac tggcgcactc tttctctatt tctgtttcgc actcacgctc agtgttattg
    10321 attttcgcac tcagctcctg cccgacaaac tcaccttacc gctgctctgg cttggcttgg
    10381 tatttaatgc gcagtctgga ttaattgatt tacatgatgc ggtttacggc gcggtagctg
    10441 gctatggggt gctgtggtgt gtttactggg gcgtctggtt agtttgtcac aaagagggat
    10501 tgggctacgg tgatttcaag ctactggccg ctgcaggcgc gtggtgtggc tggcaaacgt
    10561 tgccaatgat actgctaatt gcctcactgg gtggcattgg ttacgccatc gtttcacaac
    10621 ttctgcaacg ccggactata accactatcg cattcggccc gtggcttgcg ctcggcagca
    10681 tgataaacct ggggtatctg gcctggatct cgtattaacc gggagggttc tccctcccga
    10741 cacggctcaa ctttcttcgc ggatctgtgc ttgcagataa ttttgcaggc ccatcttctg
    10801 aatcagatca agttccgttt ccagccagtc gatatggcct tcttcatcac gcaaaatttc
    10861 tatcatcata tcgcggctga cgtaatcatg aacgctatcg gcataaccaa ttgcctcacg
    10921 caaattcttc gcgccatcca gctcaagtgc cagatcagaa cgcagcattt cctcaacatc
    10981 ctcaccaatg ttcagtttgc ccaggtcctg taaatttgga agaccttcca gaaaaagaat
    11041 gcgctcaata taacgatcgg cgtgtttcat ctcatcaatg gattcatgat actccacatc
    11101 attgagacgt ttgagacccc agtttttaaa catccgggca tggagaaagt actgattgat
    11161 tgcgacaagc tcatttccca acagtttgtt gagataattt ataaccttag tatcaccttt
    11221 cattttttga ctccttcgct tccactcttt gaagcgtaga acggcttacg agtaagtcaa
    11281 aaaacacact ccgtttatgc ggactcctta aactccggca actgcattaa ttcatcctcc
    11341 atcacttcac gcgcggcccg gacacattta ccgcactgat tgccgaccgg aataaatttt
    11401 tttaattgct ggaacgagtg aggggaaaac tggcgcacag cctgacgaat ttttttatcg
    11461 ctgat
//
LOCUS       DAZGAP010000166        11403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGAP010000166 DAZGAP010000000
VERSION     DAZGAP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.167"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..430
                     /locus_tag="ACHLAM_004050"
     CDS             <1..430
                     /locus_tag="ACHLAM_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO7855502.1"
                     /translation="GHPGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFA
                     SLNDEQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSK
                     FRALAAELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            445..1302
                     /gene="menB"
                     /locus_tag="ACHLAM_004051"
     CDS             445..1302
                     /gene="menB"
                     /locus_tag="ACHLAM_004051"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO7855503.1"
                     /translation="MIYPDEAMLYASVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1302..2264
                     /gene="menC"
                     /locus_tag="ACHLAM_004052"
     CDS             1302..2264
                     /gene="menC"
                     /locus_tag="ACHLAM_004052"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO7855504.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2261..3616
                     /gene="menE"
                     /locus_tag="ACHLAM_004053"
     CDS             2261..3616
                     /gene="menE"
                     /locus_tag="ACHLAM_004053"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO7855505.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMVEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3726..3992
                     /gene="pmrD"
                     /locus_tag="ACHLAM_004054"
     CDS             3726..3992
                     /gene="pmrD"
                     /locus_tag="ACHLAM_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO7855506.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3986..4372)
                     /gene="arnF"
                     /locus_tag="ACHLAM_004055"
     CDS             complement(3986..4372)
                     /gene="arnF"
                     /locus_tag="ACHLAM_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO7855507.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4372..4707)
                     /gene="arnE"
                     /locus_tag="ACHLAM_004056"
     CDS             complement(4372..4707)
                     /gene="arnE"
                     /locus_tag="ACHLAM_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO7855508.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4704..6356)
                     /gene="arnT"
                     /locus_tag="ACHLAM_004057"
     CDS             complement(4704..6356)
                     /gene="arnT"
                     /locus_tag="ACHLAM_004057"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO7855509.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGAMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6356..7246)
                     /gene="arnD"
                     /locus_tag="ACHLAM_004058"
     CDS             complement(6356..7246)
                     /gene="arnD"
                     /locus_tag="ACHLAM_004058"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO7855510.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7243..9225)
                     /gene="arnA"
                     /locus_tag="ACHLAM_004059"
     CDS             complement(7243..9225)
                     /gene="arnA"
                     /locus_tag="ACHLAM_004059"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO7855511.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9225..10193)
                     /gene="arnC"
                     /locus_tag="ACHLAM_004060"
     CDS             complement(9225..10193)
                     /gene="arnC"
                     /locus_tag="ACHLAM_004060"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO7855512.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10197..11336)
                     /gene="arnB"
                     /locus_tag="ACHLAM_004061"
     CDS             complement(10197..11336)
                     /gene="arnB"
                     /locus_tag="ACHLAM_004061"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO7855513.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKSTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2774 a         2967 c         3063 g         2599 t
ORIGIN      
        1 cgggcatcct gggttgcaaa atgctgaaca acgcacggaa cgtcagcgtt ccgatcgcca
       61 atgggcgcag cgttttcgca cagaaccgtt aacggcggta tttgctgact ggtatcaaca
      121 gcctgttttt gcctcactca atgacgaaca acgccgggag ctggttgcgc tgcgcagcaa
      181 caataatggc gcaacacttg ccgccatgct ggaggcgact tctctcgccg tccagcccga
      241 tttacgtgct aaccttagcg cccgcacatt tgcgttttat tatttatgtg gtgaacgtga
      301 cagcaaattc cgcgccctgg cggcggaact ggctgccgaa tgccatgtca ttcctcgcgc
      361 cggacataac gcgcaccggg aaaatcccgc tggcgtaatc gcaagtctgg cgcagatctt
      421 gcgtttctga ctaaaggaca caatatgatt tatcctgatg aagcaatgct ttacgcatcg
      481 gttgaatggc acgactgctc cgaaggtttt gaggacattc gttatgaaaa atccaccgac
      541 ggtatcgcaa aaatcaccat taatcgtccg caggtgcgca atgccttccg tcctctgacg
      601 gtaaaagaga tgatccaggc actggcagat gcgcgttatg acgacaacat cggcgtgatc
      661 attctgactg gcgcaggcga taaagcgttc tgctccggcg gtgaccagaa agtgcgtggt
      721 gattacggcg gctataagga tgattccggc gtacatcacc tgaacgtgct ggacttccag
      781 cgtcagatcc gtacctgccc gaaaccggtt gtcgcgatgg tggctggcta ctccattggt
      841 ggcggtcacg ttctgcacat gatgtgcgac ctgactatcg cggcagataa tgccatcttc
      901 ggtcagactg gcccgaaagt cggttccttc gacggcggct ggggcgcttc ctacatggcg
      961 cgcatcgtcg ggcagaaaaa agcgcgtgaa atctggttcc tgtgccgtca gtacgacgcg
     1021 aaacaggcgc tggatatggg ccttgtaaat accgtggtgc cgctggcgga tctggaaaaa
     1081 gaaaccgtgc gctggtgccg tgaaatgctg caaaacagcc cgatggcgct gcgctgcctg
     1141 aaagctgcac tgaacgccga ctgtgacggt caggcgggtc tgcaggagct ggcgggcaac
     1201 gccaccatgc tgttctacat gacggaagaa ggtcaggaag gtcgcaacgc tttcaaccag
     1261 aaacgtcagc ctgatttcag caaatttaaa cggaatccgt aatgcgtagc gcgcaggtat
     1321 accgctggca gatccccatg gacgcggggg tggttctgcg cgacaggcgg ttaaaaaccc
     1381 gcgacgggct gtatgtttgc ctgcgtgagg gcgagcgcga agggtggggg gagatctccc
     1441 cactaccggg cttcagccag gaaacctggg aagaggcaca aagcgggctg cttgcctggg
     1501 taaataactg gctggcaggt gatagcgagc taccgcaaat gccttccgtc gcctttggcg
     1561 taagctgtgc attggcggag ctggcagaca cgctcccgca ggcggccaat tatcgtgcgg
     1621 caccactgtg taatggcgat ccggacgatc tgatcctgaa acttgccgat atgccgggcg
     1681 agaaagtggc gaaggtcaaa gtgggattgt acgaagccgt gcgcgacggc atggtggtca
     1741 atctgttgct ggaggcaatt ccggatctgc atttgcgtct tgacgcgaac cgcgcctgga
     1801 cgccgctgaa aggccagcag tttgccaaat acgttaaccc ggattatcgc gaccgcatcg
     1861 cgtttctcga agagccgtgc aaaacccgcg atgattcccg tgcgtttgcc cgtgaaaccg
     1921 gcattgccat tgcctgggat gaaagcctgc gcgagccgga ttttgccttt gtggctgaag
     1981 agggcgtgcg cgcggtagtt atcaaaccca cgctcacggg cagcctggag aaagtacgcg
     2041 agcaagtgca ggcggcgcat gcgctggggc tgacggcggt gatcagttct tccattgagt
     2101 cgagcttagg tctaacgcaa ctggcgcgga ttgctgcctg gttaacgcca gacaccattc
     2161 cgggactgga cacgctggat ctgatgcagg cgcagcaggt tcgtcgctgg ccggggagcc
     2221 tgctgccgtt agtggacgtt gatgcactgg agcggctgtt atgatcttct ctgactggcc
     2281 gtggcgtcac tggcggcaag tgcggggaga agccatcgcc ttacgtctta atgacgagca
     2341 actcaactgg cgcgagcttt gtgctcgcgt cgatgaatta gcctccagct ttgcgatgca
     2401 gggtgtggtt gagggcagcg gcgtgatgtt gcgggcgtgg aatacgccgc aaacgctgct
     2461 ggcctggctg gcgttactgc aatgcggggc gcgggtgctg cccgtgaatc cacagctgcc
     2521 gcaaccgtta cttgaggaat tgctccccaa tcttacgctg caatttgcgc tggtgccgga
     2581 tggggaaaac acgtttccgg cattaacgtc gctgcacatt cagcgggttg aaggcgcaca
     2641 tgccgctacg tggcagccga cgcgtctgtg ctcaatgacg ttgacctcgg gttctaccgg
     2701 gctgccgaaa gccgctgtcc atacttatca ggcccatctt gccagtgccg aaggggtgtt
     2761 atcgttgatt ccgtttggcg atcacgatga ctggttgctc tctttaccgc tgtttcacgt
     2821 ctccggtcag ggaattatgt ggcgctggtt atacgctggt gcgcggatga cggtgcgtga
     2881 taaacagcca ttggagcaaa tgctggcagg ctgtactcac gcttcactgg tgccaacaca
     2941 actctggcgt ttgctggtta accgcagttc cgtttccctg aaagcggtgt tacttggcgg
     3001 cgcggctatc ccggtcgagt tgacggaaca ggcgcgcgag caggggattc gttgcttttg
     3061 cggctatggt ctgaccgagt ttgcctccac ggtgtgtgcg aaagaagccg acggtctggc
     3121 agacgttggt tcgccgctgc cgggtcggga agtgaaaatc gttaatgatg aagtgtggct
     3181 gcgggctgct agtatggtag aaggttactg gcgtaacggg caactggttt cactggttaa
     3241 tgacgaaggc tggtacgcta cgcgcgatcg cggtgagatg cataatggca agctgaccat
     3301 tgtcggacgt ttagacaatc tattcttcag tggcggagag ggtattcagc cggaagaagt
     3361 cgagcgcgta attgctgcac atcctgcggt tttgcaggtg tttatcgtcc ccgttgccga
     3421 caaggagttt ggtcatcgac cggtggcggt ggtggagtat gaccagcaaa ccgttgatct
     3481 tgacgaatgg gtgaaagaca agctggctcg ttttcaacaa ccggtgcgct ggctgaatct
     3541 gccgccggag ctgaaaaacg gcggtattaa aatttcacgt caggcgctaa aagagtgggt
     3601 gcaacgccag caataattaa ccctccgttt cacttctcat ttctgccatt ccattgccgt
     3661 tgataaagag tttatctgtt aatctgtaaa ttgatgtgaa aactcttagc aaacaggata
     3721 atgcaatgga atggctggtc aaaaaatcgt gctgcaataa acaagataac agacatgtaa
     3781 ttatgctctg cgatgctggc ggcgcgataa aaatgatcgc cgaagtgaag agtgacttcg
     3841 ccgtgaaagt gggggattta ctctcgcctt tgcagaatgc gctttactgt attaatcgtg
     3901 aaaagctgca cacggtgaaa gtcctttctg caagctgtta tagccccgat gagtgggaac
     3961 ggcagtgtaa agcggcaggg aaaactcagt aacgttgttt tgttgtgggc aggaaaatca
     4021 gcatcaaccc gctcataata caggctactc ccagtagtgc tttcagcgaa aaagtccctt
     4081 cccagccggg aagtaccatt gaagcaatcc ataccagcac ataactcata ctgagcaagg
     4141 cataggcttt gcttagtgca agtttatgta gtgttttata ccaacaaaaa acggaaagca
     4201 gataacccag cagcccgagt aacagtatcc gggcatccag gccaaaagct aacaacgccg
     4261 cgataaaatc ccataagtgc gtcatcggcg gcagatgact cgccgcaaaa cccaggctta
     4321 attgcgcagc ggaggcgata atgacgctga ataatcccca catcaggccc attacacggt
     4381 actcccgagg atcacaatgc caccaataat gaacgccacc ccacaccagt gacgcggcga
     4441 taccggttcg tgccacagtt ttactgcagc cagcgtcacc cagacaaaat tcagacttaa
     4501 catcgggtag gcaatgccta ccggtacgtt ctgcaagacc agcagccaga gcaccatggc
     4561 aagaccaaga caagccagcg ccagtcccag ccacagcacg atatgtttgc gccgtttgtt
     4621 tatcgccaca aagcaggttg cctgtttctg acacaactgc ccggcaacgc taagcaagct
     4681 ggcaaagact aatgttagcc agatcatttg ggacgatact gaatcagcac cagacgctcc
     4741 tgacgatcga tggcatctgc gggcggaatg gcgagactgt tgatatcttc attgcggtca
     4801 accgaaagca cgagtgtaat aatcccctcc tgacgatgtt gattaagcca gtttgcgaac
     4861 tcatcaccgc tgacaaatct ccctttcgca tccggataat taaggccgta tttcaactca
     4921 cctgtctggc gatacatgat gatgtcatcg cgttgcaggc tccatgccag acctgcggca
     4981 acaccgacgc tatcagtaag aatatagcgg cttggctgaa gtgattcttg tgtcatctcg
     5041 acaaaaaatt gcggatgttt tccttccatg actctgtcag gaattgaaaa tcctaccagc
     5101 aacgccagcc ccagcgggca aagcgcggca aaagaccagg tcttttcgac gtttgttaag
     5161 gtgtaccagc cgaaaaatgc ccatagcgaa aaaatcgacc aggcacaaaa gactttatag
     5221 ctctcgaagg tttgccacac cggcgtgttc attgctcccc agggggagac cacaaatgtg
     5281 gcaataatgc cagtgacgcc aaaagcgatg ttaatccagc cattaatccg caacgccaga
     5341 ggattatttt ttgctgccag caaagcgtaa tgcgccatca gcattgccag aggagcaaag
     5401 caagaaagaa tataggtggg caatttgcct ttggcgacgg agaaaaacaa cagcggcatt
     5461 atcgtccagc tcaacaaata gacggttgcg gaatgcttgc ggtttttcca gcctgcgtac
     5521 agtgctccgg ggagtaatcc cagccacggc aggctaccgg caatgatgac cggcaagtag
     5581 taccagaacg gagctctatg ttgggcatca tccattgcaa agcgttgaat atgctcaacc
     5641 cagaaaaaat agtgccagaa gtcaggctcc cgctgcgcta tcgccagtcc ccaggggaga
     5701 accgtcagta cgcaactgat gaccgccagc cagccgtaaa taaagagatc tttccagcgt
     5761 ttttgcgttg caacccatgg aagcacgctt aataccggca cggcaagggc gagaaaacct
     5821 ttggtcatca cccccatgcc gcaagtgatt cccagcagta aaaatcccgc gcttttgcct
     5881 ttccacgtct gtgcctgcat tgccagccag aagctgcaca ttcccgccac cagccagaag
     5941 gcaataaacg gatcgagcac ggcataagtg ccaatggcat agacaataaa caatgagaga
     6001 taaatcactg cggcgagtag agccagacgt ttatcgcgcc ataagcgcag agtaaaccag
     6061 gtcaccagcg cggcagttaa cagggtcgca aagataacgc ctgcccgcac accaaagtta
     6121 ttcgcgccaa atagccattg cccaatgctg ttaatccagt atccggcaat gggtttttcg
     6181 aaataacgta gccctaacag atggggcaca atccagtcgc cggatgccag catttctcga
     6241 ctgatttccg cataacgcgt ttcatcgggt tgccatagca gacgcgtgct gatcggtaac
     6301 aggtaatagc aggcaataaa cgcgaagagg ccgataaggt aacgtaccga tttcatcagc
     6361 gactacccgc aatttgttgg caacccagcc agccttcacg tccggcaata tttccgcgaa
     6421 caacttgtcc gagcggcaac atccctgata acagttcgct taaagggcaa aatgtcacgc
     6481 cttcctgggc tgcccgttta aggagatcca caaaattatg ctgataagcg cagccttcaa
     6541 cttctgcatg aatggtataa accggcgtgc ctttatctcg caggatgcga ttgagtaacc
     6601 aaccgttaaa atcttctgct ttcacatccc gaccaatcac ttcatcccag gtgggtaagg
     6661 tcaccggaat ttgcgcagta ccagggtttc ctgattcgag cagtggacga aacggcatcg
     6721 ccccacgaca atcgctgttg tagcgcaaat ggaacgcttc ttttgcttcg atcacctgct
     6781 ggtcggcacg ccagccagcg gctgcggagc aggttaccgg ttggccgata atttcttcca
     6841 gggtgcgaag gccgcgtgcg atatcgtcga tcattgtttg ccgatcccaa ttaccgctat
     6901 gggcttgcca ggcatggtga tcccaggcgt gcaggccgac ttcgtgatgt ttagccgctt
     6961 cacgaatgat atcggcattg gcatgaccaa tctctttacc cggccaggcc gtacctgcca
     7021 gtaaaatatc ccagccataa agcgatgccg cgtttgagcg cagcatcttc cacaaaaact
     7081 gtggcttcac cagtcgccag agatggcggc ccatattgtc cgggccgacg ctgaaaaaaa
     7141 tgctggcctg aatattatgc ttactcaaga tttccagcag acgcggcacg ccttcacggg
     7201 tgccacgaaa ggtatcgaca tcaatgcgta agcctacttt ggtcatgatg gtttatccgt
     7261 aagatcaacg gtgcgcagga agaaatccag cgtttcgtcg atggtttcct gcatatcaat
     7321 tttcggttcc cagtccaggc agcggcgggc attgcggatg ctcggtttac gatgctctac
     7381 gtcctgatat cctttgccat agtagctgct actttcgaca acacgaaagc ctgcaaacgg
     7441 cgggaagtga tggcgcagcg gatgtttttc gaagctcgcc agcagcatct cgcccagttc
     7501 ctcaatgctc gcttcgttct caggattgcc aatgttgata atttcgccgt cgcagcgatt
     7561 tcccgcattt tcgataatgc ggtacaacgc ctcgatacca tcccggatat cagtaaagca
     7621 gcgtttttgt tttccgccat caattagctt aattggtgaa ccttctacca gattgagaat
     7681 gagttgcgta atagcgcggg agctgccaat tcgcgctgca ttaaggttat ccagtcgtgg
     7741 tcccatccag ttaaacggcc ggaagagggt gaactgtaaa ccctcttttt cgccataggc
     7801 ccagatcacc cgatcaagta attgttttga caccgaataa atccagcgtg gtttattcac
     7861 cgggccgacg gttaaattag aatggtcctc atcgaagtat ttatcgctac acatcccata
     7921 aacttctgaa gttgacggga agatgattcg cttacggtac ttcacgcagt agcggataat
     7981 gcgcagattc tcttcaaaat cgagttcaaa tacgcgcagc gggttgcggg tatattcaat
     8041 cggcgtggct atcgccacca gcggcaagac gacatcacat tttttgacgt gatactcaat
     8101 ccattcggaa tgaatactga tatcgccttc gacaaagtga aaatgcggat gattcagaaa
     8161 acggcttatc gcatcgctgc caatatccag accgtaaact tcataatgat cttcgcgcag
     8221 caggcgttct gtcagatggt tgccaataaa gccattcacc ccgaggatga gtacccgggt
     8281 acggcgtcgg gcggcacagg caggctggct attcaagcgt gaaccttgca ccaggcccag
     8341 cgtctgcgct aattgcgagc cctgcatagt aatgccgtcg cccgcctgtc cggtgacgat
     8401 ttccagcgcg ccatcgccac aggcaatcag cagtggcgct acagaaatca cgctccccgg
     8461 ctgtgctttg ctggcatgag gatgaacacg cgacgaccag acggtgaatt tctgattgcc
     8521 aacatagctg aaggcacccg gccacggatc ggcaacggca cgtaccatgt tgtgcagtac
     8581 ggatgccggc ttatgccatt cgaggaaact gtcatccggc gttctgcgac caaaacaggt
     8641 agcttcgttt tcgcgctggg cgatttccag aatattaccg tgtttaatgg tgggtaatgt
     8701 ctgctccagt agctggcgcg cggcatgaca taacttatga tgcaacgtaa tggcgatatc
     8761 gtctggcgca atggcaatgc gcagttgcgc cacaatggcc ccggcatcgg cacgtttcac
     8821 cattcggtgc aatgtaacgc cagtttccgt ttcaccgttg accagcaccc agttcagcgg
     8881 cgcgcgacca cgatattttg gtaacagcga accatgcaga ttaaatgcac ctgcgggagc
     8941 gagctgcaaa atctcgtcgc aaataagatg gcgataataa aaagagaaaa tcacctctgg
     9001 tgagagttgg gcaatacgtt ccacccacag cggatgatta acgttatctg gcgcataaac
     9061 cggaatgcct ctttccgccg ccagacgagc taccgaacca taaaaggctt tttcaccggg
     9121 attatcggta tgagtaaaaa tagcgctaat ttcgtaaccg gcagccagca gggcttcaat
     9181 accgaggcat cccatatcgt ggtaggcaaa aacgacggtt ttcattattc attttccttg
     9241 ctggatggac ggataacttg ctgaacaaaa tagcgggggc gggcgcggac atcggtgtag
     9301 atcctgccga tatattcacc gagtaatccc atgccgataa actgagcgcc aataaaagta
     9361 aacagcacgg caaatagcat aaagacgcct tctgccgccc attgtggtcc gaaggtcagg
     9421 cgtaaaatca ccagcagcac cgcaatgcta aaacctccaa tcgcaataat gctgccgagc
     9481 agactcagca tacgtagcgg cgttgtggta aggcaggtca ccaggtcgta catcaaatta
     9541 atcaggcgca taaaactgta tttggattca ccaaactcac gctcggcatg gtgcactgga
     9601 atttcaatgg cacggcgggc gaagatattc gccagaatcg ggataaaggt actgcgttca
     9661 tggcagtgca acatcgcatc gacaatatga cggcgatagg cgcgcagcat acaaccgtag
     9721 tcacccatcg ctttgccggt ggtgcgctga ataagccggt taatcatctt cgaagcggtt
     9781 ttacgaaacc agctgtcctg gcggttctgg cgtacagtcc ctaccacgtc gtaaccttca
     9841 tcggcttttg ccaccagacg ggggatttcc tctggcggat tctggagatc ggcatcgagg
     9901 gtaataatta agtcgccagt aacgtgactg aatcccgcca taatcgctga atgttgcccg
     9961 taattgcggt taagcaaaat agacacaata tggctgttct ccgcttgtga ggcttcgacc
    10021 agcatatgcg cggaattatc gctactgccg tcatcaatca gcaggatttc atactctttc
    10081 cccagtgatt cacaggctgc ggtggtgcgc ctgattaatt ccggtaagct ttcctgctcg
    10141 ttataaacgg gaataaccac cgagactttc ttaacagggt ggatttcaaa catggcttat
    10201 tgtcctgcga gttgctgaag ggctgtgatg acgcggtcgg catcggcggt ggtcatatcc
    10261 gggaacagcg gcaacgaaca gatgcgttcg ctattccatt cggtattcgg taacgacagc
    10321 gtgggaaaac gctcgcgata atatttttgt gtgtgagcgg cgcggaaatg taacccggta
    10381 ccaatgcctc tttcttttaa cgcttccatc aacgcatcgc gactgatacc acaacgttgt
    10441 tcatcgacac gaataataaa cagatgccag gcgtgaacgt gtgaccaggc gggaaggctt
    10501 aatggctgaa aggggagagc tgccagtgct tgctgatatt gctgggcaat ttcgcgccga
    10561 cgggtgttga ggtgctctaa tttgactaac tgtgtcaggg caatcgcggc gttaatatcg
    10621 gtcagattgt acttatagcc cggcgttaag acttcagcct gcggtgcacg gccccaggta
    10681 tgtctgtcat aggcatcgac gcccagaccg tgaaatttta gcatccgtag ctggcgggca
    10741 aggttttcat tatctgtaac aatcaggcca ccttccgcac aggtaatatt tttgatggcg
    10801 tgaaacgaaa aaatagcggt actttttgcg ccaatatgtc gccctttgta atacgtacct
    10861 acggcatggg cagcatcttc gataactgcg atgccgtaac gttcgccaat ggcgcgaatg
    10921 gcgtcaatat ctgctggcgc accggcataa tgcaccggaa tgatggcttt agtgcgtggc
    10981 gtaatggctg actcgatcgc ttcaggcgtg accatcagcg tatcgcggtc gacatccacc
    11041 attaccggcg ttgcacccaa caaggagatc atattgaggg ttgaaaccca ggtcagggaa
    11101 ggcgtaatca cttcatcgcc cttgccaatt tccaacgcca ttagcgtgat atgcattccg
    11161 gcggtggctg aactgaccgc gatggcatgc tgatttcccg tcaactggca aaaggcttgt
    11221 tcaagcgcct gattcttcgg accggttgtg atccaaccgg attcgagaac ctctttaact
    11281 gctgcgagtt cctccacgcc cattgctggt cgcgaaaaag gcaaaaattc tgacattgct
    11341 tttccttccg ccattgaata cctgtccact tatatttgct ataaagagtg ttgtgtatat
    11401 ttt
//
LOCUS       DAZGAP010000167        11320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGAP010000167 DAZGAP010000000
VERSION     DAZGAP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.168"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            171..470
                     /gene="yoaC"
                     /locus_tag="ACHLAM_004062"
     CDS             171..470
                     /gene="yoaC"
                     /locus_tag="ACHLAM_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO7855514.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(590..769)
                     /gene="yoaH"
                     /locus_tag="ACHLAM_004063"
     CDS             complement(590..769)
                     /gene="yoaH"
                     /locus_tag="ACHLAM_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO7855515.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            843..2204
                     /gene="pabB"
                     /locus_tag="ACHLAM_004064"
     CDS             843..2204
                     /gene="pabB"
                     /locus_tag="ACHLAM_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO7855516.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEGFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2207..2785
                     /gene="nudL"
                     /locus_tag="ACHLAM_004065"
     CDS             2207..2785
                     /gene="nudL"
                     /locus_tag="ACHLAM_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO7855517.1"
                     /translation="MEHRSLTLDDFLSRFQLLRPQMNRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2969..4333
                     /gene="sdaA"
                     /locus_tag="ACHLAM_004066"
     CDS             2969..4333
                     /gene="sdaA"
                     /locus_tag="ACHLAM_004066"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7855518.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4464..6062
                     /gene="pdeD"
                     /locus_tag="ACHLAM_004067"
     CDS             4464..6062
                     /gene="pdeD"
                     /locus_tag="ACHLAM_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7855519.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLLRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6066..7622)
                     /gene="yoaE"
                     /locus_tag="ACHLAM_004068"
     CDS             complement(6066..7622)
                     /gene="yoaE"
                     /locus_tag="ACHLAM_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO7855520.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7610..7768)
                     /gene="yoaL"
                     /locus_tag="ACHLAM_004069"
     CDS             complement(7610..7768)
                     /gene="yoaL"
                     /locus_tag="ACHLAM_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO7855521.1"
                     /translation="MDRHRRHFSIRPFNVCLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8085..9056
                     /gene="manX"
                     /locus_tag="ACHLAM_004070"
     CDS             8085..9056
                     /gene="manX"
                     /locus_tag="ACHLAM_004070"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO7855522.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9119..9919
                     /gene="manY"
                     /locus_tag="ACHLAM_004071"
     CDS             9119..9919
                     /gene="manY"
                     /locus_tag="ACHLAM_004071"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO7855523.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9932..10783
                     /gene="manZ"
                     /locus_tag="ACHLAM_004072"
     CDS             9932..10783
                     /gene="manZ"
                     /locus_tag="ACHLAM_004072"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO7855524.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10838..11296
                     /gene="yobD"
                     /locus_tag="ACHLAM_004073"
     CDS             10838..11296
                     /gene="yobD"
                     /locus_tag="ACHLAM_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO7855525.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2780 a         2861 c         2842 g         2837 t
ORIGIN      
        1 attaattttc tccatcttac tggataacac actatgagct gctaccttta aaacacctga
       61 atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag atacaaatta
      121 aggtcatata tagccttatc gataacatgg ttaattttaa ggataagaat atgcctgctg
      181 taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca acaccaagtt
      241 cgatgaatga aagtacggcg aagggaatct ttaaatattt aaaagaactg ggagtacccg
      301 cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct ggtttcacgg
      361 aaaaaatggt tggatgggca aaaaaaatgg agtctggtga acgctctgtg attaaaaatc
      421 ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtttga gttaaattta
      481 catcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc tttttctcac
      541 aatcaaataa tcatcggctt ctataacgaa agcgatacgg attagtgtgt tactcgtctt
      601 catcctcaaa acgcgccacg attcgctcgc cggcatggtt ggcacgcagc tcttctgcca
      661 ccaacgcgat tgcctggccg ctgctcatcc cttgagccat cagttcctgg atccgctcga
      721 cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct
      781 gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta gagtcaggac
      841 tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac gccgctgaat
      901 tttatttctc acgcttaagc cacctgccgt gggcgatgct tttacactcc ggctatgctg
      961 atcatccata tagccgcttt gatattgtgg tcgccgagcc gatttgcact ttaaccactt
     1021 tcggtaaaga aaccgttgtt agtgaaaacg aaaaacgcac aacgaccact gatgacccgc
     1081 tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccagcgcat aacgaagatt
     1141 tgccatttca gggcggcgcg ctggggttgt ttggctacga tctgggccgc cgttttgagt
     1201 cactgccaga aattgcgcag caagatatcg ttctgccgga tatggcagtg ggtatctacg
     1261 actgggcgct ggttgttgac caccagcgtc aaacagtttc tttgctgagt cataatgatg
     1321 tcaatgctcg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa ggtttcacgc
     1381 tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa tttcgccagg
     1441 tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag cgttttcatg
     1501 cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc aaccgcgcgc
     1561 catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg ccagagcggt
     1621 ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg ctaccacgcc
     1681 tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca gcgaaagatc
     1741 gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgc gttgccgtag
     1801 ccggttcggt aaaagtacca gagctgttcg tggtcgaacc cttccctgcc gtgcatcatc
     1861 tggtcagcac cataacggcg cggctaccag aacagttaca caccagcgat ctgctgcgcg
     1921 cagcttttcc tggtggctca ataactgggg ctccgaaagt acgggctatg gaaattatcg
     1981 acgaactgga accgcaccga cgcaatgcct ggtgcggcag cattggctat ttgagctttt
     2041 gcggcaacat ggataccagc attactatcc gcacgctaac tgccattaac ggacaaatat
     2101 actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa tatcaggaaa
     2161 cttttgataa agttaataag atcctacgtc aactggagaa ataaacgtgg aacaccgtag
     2221 cctgacgctt gatgattttt tatcgcgctt tcaacttttg cgtccgcaaa tgaaccggga
     2281 aacgttaaat catcgtcagg ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg
     2341 gttgttgctg actcagcgtt cgattcatct gcgtaaacac gctggacaag tggcattccc
     2401 tggaggcgca gtcgatgaca cggacgcatc agttatcgcc gccgcgctgc gcgaagctga
     2461 agaagaggtc gctataccgc cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga
     2521 tagcgtaact ggctaccagg taaccccagt ggtcggcatt atcccgcccg atctgccgta
     2581 tcgcgccagt gaagatgaag tctcggcggt gtttgaaatg ccgctcgccc aggcattaca
     2641 tctgggtcgt tatcatcctt tagatatcta ccgccgtggc gattcacatc gggtatggct
     2701 gtcctggtac gaacagtatt ttgtatgggg aatgaccgca ggcataattc gtgagctggc
     2761 gctgcaaatt ggtgtgaaac cctgactata cttatcttta catctacaaa acactacttg
     2821 agacaatcat cgcaatatta gttaaatcgc ggtttttgat tagtttaatt catgtgaata
     2881 gttaagccag tcgccgcgtt ccctcttaca ctatgcgctg ttattagttc gttactggaa
     2941 gtccagtcac cttgtcagga gtattatcgt gattagtcta ttcgacatgt ttaaggtggg
     3001 gattggtccc tcatcttccc ataccgtagg gcctatgaag gcgggtaaac agttcgtcga
     3061 tgatctggtc gaaaaaggct tactggatag cgttactcgc gttgccgtgg acgtttatgg
     3121 ttcactgtcg ctgacgggta aaggccacca caccgatatc gccattatta tgggtcttgc
     3181 aggtaacgaa cctgccaccg tggatatcga cagtattccc ggttttattc gcgacgtaga
     3241 agagcgcgaa cgtctgctgc tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa
     3301 cgggatgcgt tttcataacg gtaaccttcc gctgcatgaa aacggtatgc aaatccacgc
     3361 ctataacggc gatgaagtcg tctacagcaa aacttattat tccatcggcg gcggttttat
     3421 cgtcgatgaa gaacactttg gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt
     3481 caaatctgcc accgaactgc tcgcgtactg taatgaaacc ggctattcgc tttctggtct
     3541 cgctatgcaa aacgaactgg cgctgcacag caagaaagag atcgacgagt atttcgcgca
     3601 tgtctggcaa accatgcagg catgtatcga tcgcgggatg aataccgaag gcgtactgcc
     3661 aggcccgttg cgcgtgccac gtcgtgcgtc ggccctgcgc cggatgctgg tttccagcga
     3721 taaactgtct aacgatccga tgaatgtcat tgactgggta aacatgtttg cgctggcagt
     3781 taacgaagaa aacgccgccg gtggccgtgt ggtaactgcg ccaactaacg gtgcctgcgg
     3841 tatcgttccg gcagtgctgg cttactatga ccactttatt gaatcagtta gcccagacat
     3901 ctatacccgt tactttatgg cagcgggcgc gattggtgca ctgtataaaa tgaacgcgtc
     3961 tatttccggt gcggaagtcg gttgccaggg cgaagtaggt gttgcctgtt caatggctgc
     4021 tgcgggcctt gccgagctgt tgggcggtag cccggaacag gtttgtgtgg cagcggaaat
     4081 tggcatggag cacaacctcg gtctgacctg cgaccccgtt gcagggcaag ttcaggtgcc
     4141 gtgcattgaa cgtaatgcca ttgcctctgt gaaggcgatt aacgccgcac ggatggctct
     4201 gcgccgcacc agtgcaccgc gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac
     4261 cggtaaggac atgaacgcta aataccgcga aacctcacgc ggtggtctgg caatcaaagt
     4321 ccagtgtgac taatacttct tactcgccca tctgcaacgg atgggcgaat ttattcccgc
     4381 tttctcgtct gctgtaattt tccccactac acttccactg ttgcgtcagg cgtttgtcgc
     4441 catacgctta cagggtggcc cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc
     4501 gtaatcgaat gattgtttgt acgatttgcg cactcgttac gctcgcttcg accctgagcg
     4561 tgcgatttat ttcacagcgt aacttaaatc aacaacgggt agtacaattc gccaatcacg
     4621 ctgtagagga attagataaa gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc
     4681 cgctgattgg tctgccctgc tctgtcgccc atttgccatt acgtaaacag gcggcaaaac
     4741 tccaaactgt gcgatccatt ggcctggtgc aagacggcac actttattgc tccagcattt
     4801 ttggttatcg caatgtgccc gtcgtggaca ttctggctga acttcctgca ccgcaaccac
     4861 ttttacgcct gacgaccgac catgccctga ttaaaggcag tccggttttg attcaatgga
     4921 cgccagcagc gggcagtagc aaagctgggg tcatggagat gattaacatc gacttactgg
     4981 cggcaatgct gcttgagcca caactgccgc aaatcagtag cgccagcctg acggtggacg
     5041 atcggcattt gctctatggt aatgggctgg tagattccct tccgcaacct gaaaacaatg
     5101 aaaactatca ggtttcttcg caacgctttc cttttaccat taacgttaat ggtccggggg
     5161 ctacggcgct ggcatggcac tatcttccaa cacaattacc gctggcggtg ctgctaagtt
     5221 tacttgtggg ctatatcgcc tggctggcga ccgcttaccg gatgagtttt tcccgcgaaa
     5281 tcaatctggg cctggcgcaa catgagttcg aattgttctg ccagcctttg cttaatgcgc
     5341 gcagccagca atgtattggc gtagagattt tgctgcgctg gaacaatccg cgtcagggct
     5401 ggatttcgcc ggatgtgttt attcctatcg cggaagaaca tcatttaatt gtgccactga
     5461 cccgctatgt gatggcagaa accattcgtc agcgccatgt tttcccgatg agtagtcagt
     5521 ttcatgttgg cattaacgtc gcacccagcc attttcgccg tggtgtgctg ataaaagatc
     5581 tcaatcagta ctggtttagc gctcacccga ttcagcaact gatcctcgaa atcaccgaac
     5641 gcgatgcctt actggatgtt gattatcgga ttgcccgcga gctacatcgt aaaaacgtca
     5701 aactggcgat tgatgacttc ggcaccggca atagttcatt ttcctggctt gaaacattac
     5761 gtcctgacgt gctgaaaatt gataagtcat ttaccgcagc tataggttct gacgcggtta
     5821 actcgacggt gaccgatatc atcatcgcac tggggcaaag actgaatatt gaactggtgg
     5881 cggagggtgt ggaaacgcaa gaacaggcga aatatttgct ccgtcatggc gtccatattt
     5941 tgcaagggta tttgtacgca cagccgatgc cactacgtga ttttcccaaa tggctggcgg
     6001 gcagccaacc gccgcccgcc cggcataatg gacatatcac acccattatg ccgttacgtt
     6061 aacgcttact catcttcatc gtgcgccggt tgctctttaa caatgcgaac cagatcaaca
     6121 cgataatcat tggcttcaat gatggtgata tgcagtggcc ctacatcaat cacatcgccc
     6181 acacggggaa tgtgaccatt tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg
     6241 tcatcggcaa ggtgctcaac atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct
     6301 tttaccagcc agccgtcacc gtcggtaatg atttccggcg tttcgtcagc gtccgggaat
     6361 tcacccgcaa tggcttccag cacatccagc ggcgtgacca agccttgtac cacaccaaac
     6421 tcgttggtca cgataacaaa gctcccgcga gcacgacgca gcacgcccag caggttgatc
     6481 ggatcgaggg tttccgggac gataatcgcc ggagacgccg aagcaatcgc cgccacatca
     6541 acgccctctt ccagcgccac cagcagttct ttagcacgta cgattccgat gatttcatcc
     6601 agttcaccgc gacataccgg gaacaggctg tgcggtgagg agagcagctg ctcgcggatt
     6661 tcatctaccc cgagattagc gtcaacccag cttatttcac cgcgcggcgt catgatcccg
     6721 cgcagagaac gcgacgccag cgtcagtacg ccgttaatca tgtaacgttc ttcttcggca
     6781 aatgcacctt ccgggatcgg catcggcatc gggttatcgg catcgtgctg aacattggcc
     6841 tgacgtttcc cgcccatcaa acgcaggatg gcatcggcag tacgcgctcg cagcggcaaa
     6901 gtcgactggt gacgaataaa gttgcgacgc gcaatctggt taaacacttc gatgatgatc
     6961 gagaagccaa tcgcggcata caggtaacct ttcggaatgt ggaaaccgaa accttctgcc
     7021 accagactca gaccaatcat taacaggaag ctcagacaga gcaccaccac cgtggggtgc
     7081 tggttaacaa atcgcgtcag cggtttcgat gccagcagca taactgccat cgcaatcact
     7141 accgccgcca tcatcaccgg caaatggtta accatcccta ctgcagtaat taccgcatcc
     7201 aacgagaaga cggcgtcaag gatgacaatc tgtgtgacga ccacccagaa actggcgtag
     7261 cctttaccgt ggccggaatc atgatcgcgg ttttccagcc gttcatgcag ttcggttgtt
     7321 gctttgaaca gcaagaatat gcccccgaac aacataatca ggtcgcgtcc ggagaaggag
     7381 aaatccatga cggtaaatag cggtttggtc agcgtgacca tccatgaaat cagcgacagc
     7441 agccccagac gcataatcag cgccagtgat aatcccagca aacgcgcttt atcgcgttgt
     7501 tttggcggca gtttgtcagc aagaatggca atgaagacca ggttatcgat acccagcaca
     7561 atttcgagaa caacaagcgt gagtagcccc gcccaaattg aggggtccat taagaattcc
     7621 atgacaagct cctgcttaag gaatagctat tcgacgccag aaataatgca ggcgtaacga
     7681 caaaatgcaa acgaaaggtg cggcatagag tgccagaaag gcagacgtta aaaggcctga
     7741 tgctgaaatg acgtcggtga cgatccatac tgcgggctac tgccctatac tccatggttg
     7801 ttaaacggga gttaaacata tcagagacgc ctctgatttg gcaaagattt accttccttt
     7861 gcaaacgaat gtgacaagga tattttacct ttcgaaattt ctgctaatcg aaagttaaat
     7921 tacggatctt catcacataa aataattttt tcgatatcta aaataaatcg cgaaacgcag
     7981 gggtttttgg ttgtagccct tatctgaatc gattcgattg tggacgacga ttcaaaaata
     8041 catctggcac gttgaggtgt taacgataat aaaggaggta gcaagtgacc attgctattg
     8101 ttataggcac acatggttgg gctgcagagc agttgcttaa aacggcagaa atgctgttag
     8161 gcgagcagga aaacgtcggc tggatcgatt tcgttccagg tgaaaatgcc gaaacgctga
     8221 ttgaaaagta caacgctcag ttggcaaaac tcgacaccac taaaggcgtg ctgtttctcg
     8281 ttgatacatg gggaggcagc ccgttcaatg ctgccagccg cattgtcgtc gacaaagagc
     8341 attatgaagt cattgcaggc gttaacattc caatgctcgt ggaaacgtta atggcccgtg
     8401 atgatgaccc aagctttgat gaactggtgg cgctggcagt agaaacaggt cgtgaaggcg
     8461 tgaaagcact gaaagccaaa ccggttgaaa aagccgcgcc agcacccgct gccgcagcac
     8521 caaaagcgac tccaactccg gcaaaaccaa tgggaccaaa cgactacatg gttattggcc
     8581 ttgcgcgtat cgacgaccgt ctgatacacg gtcaggtcgc cacccgctgg accaaagaaa
     8641 ccaatgtctc ccgtattatt gttgttagtg atgaagtggc tgcggatacc gttcgtaaga
     8701 cactgctcac ccaggttgca cctccgggcg taacagcaca cgtagttgat gttgccaaaa
     8761 tgattcgcgt ctacaacaac ccgaaatatg ctggcgaacg cgtaatgctg ttatttacca
     8821 acccaacaga tgtagagcgt ctcgttgaag gcggcgtgaa aatcacctct gttaacgtcg
     8881 gtggtatggc attccgtcag ggtaaaaccc aggtgaataa cgcggtttcg gttgatgaaa
     8941 aagatatcga ggcgttcaag aaactgaatg cgcgcggtat tgagctggaa gtccgtaagg
     9001 tttccaccga tccgaaactg aaaatgatgg atctgatcag caaaatcgat aagtaacgta
     9061 ttgtgttgat tatcactcag ttttcacact taagtcttac gtaaacagga gaagtacaat
     9121 ggagattacc actcttcaaa ttgtgctggt atttatcgta gcctgtatcg caggtatggg
     9181 atcaatcctc gatgaatttc agtttcaccg tccgctaatc gcgtgtaccc tggtgggtat
     9241 cgttcttggc gatatgaaaa ccggtattat tatcggtggt acgctggaaa tgatcgcgct
     9301 gggctggatg aacatcggtg ctgcagttgc gcctgacgcc gctctggctt ccatcatttc
     9361 tactattctg gttatcgcag gtcatcagag cattggtgca ggtatcgcac tggcaatccc
     9421 tctggccgct gcgggccagg tactgaccat catcgttcgt actattaccg ttgccttcca
     9481 gcacgctgcg gataaggctg ctgataacgg caacctgaca gcgatttcct ggatccacgt
     9541 ttcttctctg ttcctgcaag caatgcgtgt ggctattccg gccgtcatcg ttgcgctgtc
     9601 tgttggtacc agcgaagtcc agaacatgct gaatgcgatt ccggaagtgg tgaccaatgg
     9661 tctgaatatc gccggtggca tgatcgtggt ggttggttat gcgatggtta tcaacatgat
     9721 gcgtgctggc tacctgatgc cgttcttcta cctcggcttc gtaaccgcag cattcaccaa
     9781 ctttaacctg gttgctctgg gtgtgattgg tactgttatg gcagtgctct acatccaact
     9841 tagcccgaaa tacaaccgcg tagccggtgc gcctgctcag gcagctggta acaacgatct
     9901 cgataacgaa ctggactaac aggtgagcga aatggttgat acaactcaaa ctaccaccga
     9961 gaaaaaactc actcaaagtg atattcgtgg cgtcttcctg cgttctaacc tcttccaggg
    10021 ttcatggaac ttcgaacgta tgcaggcact gggtttctgc ttctctatgg taccggcaat
    10081 tcgtcgcctc taccctgaga acaacgaagc tcgtaaacaa gctattcgcc gtcacctgga
    10141 gttctttaac acccagccgt tcgtggctgc gccgattctc ggcgtaaccc tggcgctgga
    10201 agaacagcgt gctaatggcg cagagatcga tgacggtgct atcaacggta tcaaagtcgg
    10261 tttgatgggg ccgctggctg gtgtaggcga cccgatcttc tggggaaccg tacgtccggt
    10321 atttgcagca ctgggggccg gtatcgcaat gagcggcagc ctgttaggtc cgctgctgtt
    10381 cttcatcttg tttaacctgg tgcgtctggc aacccgttac tacggcgtag cgtatggtta
    10441 ctccaaaggt atcgatatcg ttaaagatat gggtggtggc ttcctgcaaa aactgacgga
    10501 aggggcgtct atcctcggcc tgtttgtcat gggggcatta gttaacaagt ggacgcatgt
    10561 caacatcccg ctggttgtct ctcgcattac tgaccagacg ggcaaagaac acgttaccac
    10621 tgtccagact attcttgacc agttaatgcc aggcctggta ccactgctgc tgacctttgc
    10681 ttgtatgtgg ctgttgcgca aaaaagttaa cccgctgtgg atcatcgttg gcttcttcgt
    10741 catcggtatc gctggttacg cttgcggcct gctgggactg taagactgtt gtacactacc
    10801 ggggcctttt ggccccgttt ttttatctgg aggattaatg acaatcacgg acctggtact
    10861 gattcttttc atcgccgcac tcctggcctt cgcgatctac gatcagttca tcatgccccg
    10921 ccgtaacggc cccaccctgc tggcaattcc tttgctccgg cgtggtcgca tcgatagcgt
    10981 tatcttcgtc ggattgattg tcattcttat ctataacaac gtcacgaatc atggcgcgct
    11041 aataacaaca tggttattaa gtgcactggc tctgatgggt ttttatatat tctggatccg
    11101 cgttccgaag atcatcttta aacaaaaagg ttttttcttc gccaatgtct ggattgaata
    11161 tagccgaatc aaagcgatga acttgtcgga agatggcgtg ttggtgatgc aattagaaca
    11221 gcgccggctg ttaatccgcg ttcgaaatat cgacgatctg gaaaaaattt ataagcttct
    11281 cgtttcaact caataagtta tgaatttagc caaagctatg
//
LOCUS       DAZGAP010000168        11287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGAP010000168 DAZGAP010000000
VERSION     DAZGAP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.169"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            237..1808
                     /gene="garD"
                     /locus_tag="ACHLAM_004074"
     CDS             237..1808
                     /gene="garD"
                     /locus_tag="ACHLAM_004074"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO7855526.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAIP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1957..2292
                     /gene="prlF"
                     /locus_tag="ACHLAM_004075"
     CDS             1957..2292
                     /gene="prlF"
                     /locus_tag="ACHLAM_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO7855527.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2292..2756
                     /gene="yhaV"
                     /locus_tag="ACHLAM_004076"
     CDS             2292..2756
                     /gene="yhaV"
                     /locus_tag="ACHLAM_004076"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO7855528.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPTFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2811..3620)
                     /gene="agaR"
                     /locus_tag="ACHLAM_004077"
     CDS             complement(2811..3620)
                     /gene="agaR"
                     /locus_tag="ACHLAM_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO7855529.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3869..5149
                     /gene="kbaZ"
                     /locus_tag="ACHLAM_004078"
     CDS             3869..5149
                     /gene="kbaZ"
                     /locus_tag="ACHLAM_004078"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO7855530.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5172..5645
                     /gene="agaV"
                     /locus_tag="ACHLAM_004079"
     CDS             5172..5645
                     /gene="agaV"
                     /locus_tag="ACHLAM_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO7855531.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5656..6435
                     /gene="agaW"
                     /locus_tag="ACHLAM_004080"
     CDS             5656..6435
                     /gene="agaW"
                     /locus_tag="ACHLAM_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO7855532.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6425..7303
                     /gene="agaE"
                     /locus_tag="ACHLAM_004081"
     CDS             6425..7303
                     /gene="agaE"
                     /locus_tag="ACHLAM_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO7855533.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7321..7755
                     /gene="agaF"
                     /locus_tag="ACHLAM_004082"
     CDS             7321..7755
                     /gene="agaF"
                     /locus_tag="ACHLAM_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO7855534.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7752..8885
                     /gene="nagA"
                     /locus_tag="ACHLAM_004083"
     CDS             7752..8885
                     /gene="nagA"
                     /locus_tag="ACHLAM_004083"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7855535.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHAQQIWIQGQLASF"
     gene            9235..10389
                     /locus_tag="ACHLAM_004084"
     CDS             9235..10389
                     /locus_tag="ACHLAM_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO7855536.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10402..11262
                     /gene="kbaY"
                     /locus_tag="ACHLAM_004085"
     CDS             10402..11262
                     /gene="kbaY"
                     /locus_tag="ACHLAM_004085"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO7855537.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2696 a         2918 c         2998 g         2675 t
ORIGIN      
        1 taatttaatt agtactattt aaatattatt ttgagcatat gcacataagg ttgcgcgcta
       61 aagcacagat ttgcgcttta ccttgccggg cggcactgca atccctgaaa tgattgacat
      121 tgatcacatt tctgcattta aactcctgac attcttattt cacccaatga agttatttat
      181 ttttaaatga gaccaggtcc tcattttaat aacccctggc tggagaatat tgcacaatgg
      241 ccaacatcga aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag
      301 ataatgtggc aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg
      361 ggctggaatt aattgaacat attccccagg ggcataaagt cgcattgctg gacattccgg
      421 ctaatggtga aattattcgt tatggcgaag tgattggtta cgccgtgcgc gcaatcccac
      481 gcggaagctg gatcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc
      541 tgccactggc aaccaaagtc ccggcaccct taccgccact ggaaggatac acctttgagg
      601 gctatcgcaa tgccgatggc agcgtgggca ccaaaaacct gctcggcatc accaccagcg
      661 tccactgtgt ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc gatctgctgc
      721 cgaaataccc gaacgtcgat ggcgtggtgg ggctgaatca tctgtacggt tgtggcgtgg
      781 cgattaacgc tccggcggcg gtggtgccca ttcggaccat tcacaacatt tcgcttaacc
      841 caaactttgg cggcgaggtg atggtgattg gcctgggttg cgagaaatta cagccagaac
      901 gcctgctggt cggcacggat gatgtacaag gtattccgct ggagaacgcc agtattgtca
      961 gcttgcagga tgaaaagcat gtcggttttc agtccatggt cgaggatatt ttgcaggtcg
     1021 ccgaacgcca tttgcacaaa ctgaatcaac ggcagcgaga aacctgtcca gcttcagaac
     1081 tggttgtcgg catgcagtgc ggtggcagcg atgcgttttc tggcgtaacg gcaaacccgg
     1141 cggttgggta tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttttcagaag
     1201 tcacggaagt gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaagaggtcg
     1261 gcaaacggct gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg
     1321 accgtagcgc caacccttcg ccgggcaaca agaaaggcgg tctggcaaat gtggtggaga
     1381 aagcgctcgg ctccattgct aaatcgggta aaagcgcaat tgttgaagtg ctgtcgcccg
     1441 gtcaacgccc gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct
     1501 gtggcacaca acaggtggct tcagggatta ccgtgcaagt gtttacgacc ggtcgcggta
     1561 cgccgtacgg cctgatggcg atacccgtca ttaaaatggc gacccgcacc gagctggcga
     1621 accgctggtt tgatttaatg gatattaacg cgggcactat cgctaccggc gaagaaacca
     1681 ttgaagaagt gggctggaag ttgttccact ttattctcga cgtcgccagc gggaagaaga
     1741 aaactttctc ggatcaatgg ggactgcata accagctggc ggtgtttaac ccggcaccag
     1801 tgacctgatt tcttttggaa cgtccctcgc aaaacatggc cttagtgcca tgtttttatt
     1861 gtttaaagtc cacacatcca ttaataatgc atttgcatta ccttagttca agcttataat
     1921 ttgagcagaa aacaggctgt aaaaggacag taaataatgc ccgctaatgc tcgctctaac
     1981 gctgtactga ccactgaatc aaaggtcact atacgcggac aaacaactat ccccgcgcca
     2041 gtgcgtgagg ccttaaaact gaagccaggc ctggacagca ttcattacga aattctgcct
     2101 ggtgggcaag tatttatgtg ccgactggga gatgaacagg aggatcatac tatgaatgca
     2161 tttttgcgtt ttctggatgc agatatccag aacaacccgc aaaaaactcg tccattcgac
     2221 attcaacaag gaaagaaact tgtcgctggc atggacgtca acattgacga tgagattggc
     2281 gacgacgaat aatggatttt ccacaaaggg ttaatggttg ggcgctatat gctcatccct
     2341 gttttcagga aacctacgat gctttagttg ccgaagtcga ggcattaaag ggaaaagatc
     2401 ctgaaaatta tcagagaaaa gctgccacaa agttattggc ggtggtccat aaagtgattg
     2461 aggagcatat cacggtcaat ccatcatcac cgacattccg tcatggcaag tcgttaggct
     2521 ctgggaaaaa taaagactgg tcacgggtaa aatttggtgc tggtcgctat cgtctcttct
     2581 ttcgttacag tgaaaaagag aaagtcatca ttctgggatg gatgaacgat gaaaacacgc
     2641 tgcgcaccta cggtaaaaaa acagatgcct ataccgtatt cagtaaaatg ttaaaaagag
     2701 gacatcctcc tgccgactgg gaaagcctca cccaagagac agaagaaaac cattgatggt
     2761 gttcacaatg cccgccgaac gcattggtgt tgaccagcgg gcatcctttc tcattccccg
     2821 accagaatca cttcaacccc agcctttctc agtccttcca gactatccgc aggaatgcct
     2881 tcatcaacaa tgatcatgtc gatacgttga gtatcaatga tcttatgtaa actagaacga
     2941 ttgaacttac tggaatcggt gaccacgatg atccgttccg caacttcgca catccgacgg
     3001 tttaaacggg cttcatcttc attatgcgtg ctgacgccgc gctccagatc gatcgcatct
     3061 acaccaagaa acagcatatc gaagtggtaa ttttgcagcg attgctcagc ctgatcaccg
     3121 taaaaagatt gcgactgacg gcgcaaatgc cccccggtca tcagcagctc aacgccttcc
     3181 gcttccagca acgcattagc cacgttcata ccgttggtca tcgcaattac gtcggtgtgc
     3241 ttgcgcatca gacgagcaat ctcaaaagtg gtggtcccgg agtcgaggat cacccgatga
     3301 ccaggctgaa tcaactcaac ggcagctttc gcaacgctgc gtttcatcgc ggtgttcagc
     3361 gcgcttttat cttccactga tggctcgact gacggcgttg tgctatcgca gatcaacgcg
     3421 ccaccatagg cacgcacagc gatcccctgc ttttccagaa acgccagatc gttgcggatc
     3481 gtcacagtag atacaccata caacgccgat agatcattaa cttgcacact tccttgctgg
     3541 cgcaggcgtt gaatgatctg ctctcgtcgc tcgctggtgc ctgtcactcg cttctcacct
     3601 gaagcgtcgg tattactcat agtaagtcct ttcgtaaaac tttcgtttca tttcgttttg
     3661 cctattaacg cctttctatt aagcaaatgc aagcccacct tacccattgg cgcaagctac
     3721 tctcgtttca gtggctttca ttatgtttct tttgtgaatc agatcagaaa accattatct
     3781 ttcgttttat ttttatctca ccatgacgca gtatcaactg aaacaaaacg aaagattaat
     3841 gtcgcagtaa tctgaactgg agaggaaagt gaaacatctg acagaaatgg tgagacagca
     3901 caaagcgggc aaaacaaatg ggatttatgc cgtttgttcc gcacatccgc tggtgctgga
     3961 agctgcaatc cgctacgcca gtgcaaacca aacgccgtta ctgattgaag caacctccaa
     4021 tcaggtggac cagttcggcg gttataccgg aatgacgccc gccgattttc gcggctttgt
     4081 ttgtcagctc gccgactcgt tgaatttccc gcaggatgcg ctgattctgg gtggtgacca
     4141 tctggggcca aaccgctggc aaaacctgcc tgccgctcag gcaatggcca atgccgatga
     4201 tttgattaaa agctacgtgg cggcaggatt caaaaaaatt caccttgatt gcagcatgtc
     4261 ctgtcaggac gatccgattc ccttaaccga tggcatcgtg gccgaacgcg ccgcccgtct
     4321 ggcgaaagtg gcggaagaaa cctgtcttga acactttggc gaagccgatc tggtgtatgt
     4381 cattggcacc gaagtaccgg tacctggcgg cgcgcatgaa accttaagcg agctggcggt
     4441 caccacgccg gatgccgccc gcgccacgct ggaagcccat cgtcacgcct ttgaaaagca
     4501 aggtctgagt gccatctggc cacgcatcat tgctctggtg gtacaacccg gcgtcgaatt
     4561 cgatcacacc aacgttattg attatcagcc cgtcaaagcg accgccttaa gccagatggt
     4621 cgaaaactac gaaacgctga ttttcgaagc gcactctacc gattaccaaa cgccgcaatc
     4681 gttgcgccag ctagtgattg accactttgc catcctgaaa gttggcccgg cgctgacctt
     4741 cgccctgcgt gaagctctgt tctctctggc ggcgattgaa gaagaactgg taccagcaaa
     4801 agcctgttct ggtctgcgtc aggtgctgga aaacgtgatg ctcgaccgcc cggaatactg
     4861 gcaaagccac taccacggtg acggcaacgc gcgtcgtctg gcgcgtggtt atagctactc
     4921 ggatcgcgtg cgctattact ggccggacag ccagattgat gacgctttcg ctcatctggt
     4981 tcgtaatctg gcggattcac caattccgct gccgctgatc agtcagtatc tgccgctgca
     5041 gtacgtgaaa gttcgctccg gcgagctgca gccaacgcca cgggaactca ttatcaacca
     5101 tattcaggac atcctggcgc agtaccacac agcctgtgaa ggccaataag caaaacaaag
     5161 aggaacacgc tatgccaaat attgttttaa gtcggattga tgaacgcttg attcacggtc
     5221 aggtcggcgt tcagtgggtc ggatttgcgg gggcaaatct ggtgctggta gccaacgatg
     5281 aggttgccga agatccggta caacaaaacc tgatggaaat ggtactggca gaagggatcg
     5341 ccgtacgttt ctggacgctg caaaaagtca tcgacaacat tcatcgcgcc gccgatcgac
     5401 agaaaatcct gctagtttgt aaaacaccgg ccgatttcct gacgttggtg aaaggtggcg
     5461 ttccggtgaa tcgcattaac gttggcaata tgcactacgc caatggcaag caacaaatcg
     5521 ccaaaacggt ttctgtggat gcgggtgata tcgcagcatt taacgatctg aaagccgctg
     5581 gggtggaatg cttcgttcag ggcgtcccga cagagcctgc tgtagacctc tttaaattac
     5641 tttgagggat tcatcatgga aatcagtctg ttgcaggcat ttgcgttggg aattatcgcc
     5701 tttatcgctg gcctggatat gtttaacggc ttaacccata tgcaccgccc ggtggttctc
     5761 ggcccgttgg tcgggctggt acttggcgat ctgcataccg gtattttaac cggcggtacg
     5821 ctggaactgg tgtggatggg gctggcccct ctggcgggcg cacagccgcc taacgtgatt
     5881 atcggtacta tcgtcggcac ggcgtttgcc attactactg gcgtgaaacc cgatgtcgcg
     5941 gtcggtgtcg ccgtaccttt cgctgtcgca gtacagatgg ggattacctt cctgttctcg
     6001 gtgatgtccg gcgtgatgtc ccgatgcgac cggatggcgg agaacgccga tactcgcggc
     6061 attgaacgcg tgaactatct ggcattgctg gcactcggca cgttctattt tctctgcgct
     6121 ttcctgccta tctacttcgg cgcggaacat gcaaaaacca tcattgatgt cctgccgcaa
     6181 cgattaattg atggcctcgg cgtcgcgggc ggcatcatgc cagcaatcgg ttttgccgtg
     6241 ctgctgaaaa tcatgatgaa aaacgtctac atcccctact tcatcctggg ctttgttgcc
     6301 gcggcctggc tcaagttacc ggtgctggct attgctgccg ctgcgctggc gatggcgctg
     6361 atcgacctgc tgcgtaaatc tcctgaaccg actcaacctg cggcacagaa agaggaattt
     6421 gaagatggca tctaatcaaa ccaccctgcc gaacgtcagc gaaaatgaag aaacactgct
     6481 gactggcgtc aatgaaaacg tgtatgaaga tcagagcatt ggcgcggagc tgacgaaaaa
     6541 agatatcaac cgcgtcgcct ggcgttccat gctgttacag gcttctttca actacgaacg
     6601 tatgcaggct tccggctggc tgtacggcct gctgccagca ctgaaaaaga tccacactaa
     6661 taaacgcgac ctggcgcgcg ccatgaaggg gcatatgggt ttcttcaata cccatccgtt
     6721 tctggtgacg tttgttatcg gcattatcct tgcgatggag cgttctaagc aggacgttaa
     6781 cagtattcag agcaccaaaa ttgccgtcgg tgcgccgctc ggcgggattg gcgatgcgat
     6841 gttctggcta acgctactgc cgatttgtgg cgggataggt gcaagcctcg cgctacaagg
     6901 ctctattctt ggcgctgtcg tctttattgt gctgttcaac gtggtgcacc tggggctgcg
     6961 ttttggtctg gcgcattatg cttaccgcat gggcgtggcg gcgattccac tcattaaagc
     7021 aaataccaaa aaagtcggcc atgcggcatc tatcgttggg atgacggtaa tcggcgcgct
     7081 ggtggcaacc tatgttcgtt taagcaccac gctggaaatc accgcgggcg acgcagtggt
     7141 taagttacag gctgatgtta tcgacaaact gatgccagcc ttcttaccgc tggtctacac
     7201 cctgaccatg ttctggctgg tacgccgcgg ctggagtccg ctgcgcctga ttgcagtgac
     7261 cgtggttctc ggcatcgtcg gtaaattctg ccatttcctt taaatacaat gaggtattag
     7321 atgttaagta ttattttgac agggcatggc gggtttgcca gtggcatgga aaaagcaatg
     7381 aagcaaatcc tcggcgagca atcgcagttt attgccatcg attttccgga aacctcaagc
     7441 accgcgctgc ttacatcgca gcttgaagag gccatcgccc aactggattg tgaagacggc
     7501 attgtttttc tgacggattt gctgggcggc acgccgtttc gcgtagcttc aacgctggcg
     7561 atgcaaaaac tgggctgtga agtgattacc ggcaccaatt tgcaactgct actggagatg
     7621 gtgctggagc gcgaagggtt aagcggtgaa gagtttcgtg tgcaggcgct ggagtgtgga
     7681 catcgtgggc tgaccagtct ggtggacgag ttaggccgct gccatgagga atgtccggtc
     7741 gaggaaggaa tatgacaccc gttttgcgcg ccagaaggct gctgactgaa gagggatggc
     7801 tcgatgacca tcagttgcgt attgctgacg gtgtaatcgc agaaatcgaa ccgattccag
     7861 tgggcgtgac tgaacgcgat gcggaactgc tctgccccgc ttacatcgac acccatgtac
     7921 acggtggtgc gggcgttgat gtaatggatg acgcgccgga tgtgctcgac aagctggcaa
     7981 tgcacaaagc acgcgaaggt gtcggcagtt ggttaccgac caccgtaacc gcgccgctta
     8041 ataccattca tgcggcgctg gaacgtattg ctcaacgttg ccaacgcggc ggacctggtg
     8101 cacaaatact ggggagttat ctcgaaggac cgtacttcac gccgcagaat aaaggcgcgc
     8161 atccgccgga gctgtttcgc gagctggaaa ttgccgagct ggatcaattg attgccgttt
     8221 ctcagcacac cttgcgcgtg gtggcgctgg caccggaaaa agaaggggca ttgcaggcca
     8281 tccgccatct taaacagcaa aacgtaaggg tgatgctggg gcatagcgcg gcgacctggc
     8341 aacaaacccg cgccgcgttt gatgctggtg ccgacggcct ggtgcattgc tataacggga
     8401 tgacaggttt acatcaccgc gaaccgggaa tggttggcgc gggattaacg gacaagcgcg
     8461 cctggctgga actgatagcc gatggtcatc atgtgcatcc ggcggcgatg tcgctgtgtt
     8521 gttgctgtgc gaaagagaga atcgtgctca tcaccgacgc gatgcaggca gccgggatgc
     8581 cggatggtcg ctatacgtta tgtggcgaag aagtgcagat gcacggtggc gttgtccgta
     8641 ccgcgtccgg tggactggcg ggcagtacgc tgtctgtcga tgcggcagtg cgcaacatgg
     8701 tcgagttgac gggcgtaacg cctgcggaag ccatccatat ggcgtcgctg catccggcgc
     8761 gtatgctggg tgttgatgat gttctgggat cgcttaaacc gggcaaacgc gccagcgtcg
     8821 ttgcgctgga tagcgggctg catgcgcaac aaatctggat tcagggtcaa ttagcttcgt
     8881 tttgatggtt tgctccttta ttgggccttc acttcccccg taaggccttt ctttttcttt
     8941 cgttttgatc tgtgcagcgg tgtcggatgc gacgctaatg cgccttatcc gacctacggt
     9001 tggtgaccgt aaggccggat aaagtattta cgccgcatcc agcaatccct tttacttccc
     9061 ttgtcttttc tttcaacgat cacagatttc gttttatttc ctttttctcc attgaacttt
     9121 cagtttcttt tctatagatt ttaaccaacg aaagaaatca ccaagtgaaa tgaaacgaaa
     9181 ggcaaacgaa agcgacaacg ccgacgtcaa gttcatcaga ctaaggattg agttatgcca
     9241 gaaaattaca cccctgctgc tgccgcaacc ggtacatgga ctgaagaaga gatccgccat
     9301 cagcctcgcg catggatccg ttcactcacc aacatcgacg cgctacgttc cgcgctcaat
     9361 aacttccttg aaccgttact gcgcaaagag aatctgcaaa tcatcctgac cggagccgga
     9421 acatcggcat ttatcggtga catcatcgcg ccgtggctcg ccagtcatac cggtaaaaac
     9481 ttcagcgccg taccgaccac cgatctggtc actaatccga tggactacct gaatccagct
     9541 catccgctgc tgttgatctc cttcggtcga tccggcaaca gcccggaaag cgtcgccgcc
     9601 gtggaactgg caaatcaatt tgtacccgag tgctatcacc tgccgatcac ctgcaacgaa
     9661 gcgggcgctc tttaccaaaa cgcgatcaac agcgacaacg cgtttgccct actgatgccc
     9721 gcagaaacgc acgaccgcgg ctttgcgatg accagcagca ttaccaccat gatggccagt
     9781 tgcctcgcgg ttttcgcacc tgagacgatc aacagccaga gcttccgcga cgtggcggat
     9841 cgttgccagg cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt tggttacgca
     9901 ccgtggaaac ggatcgttta tctcggcagc ggtggcttac agggcgcagc acgcgagtcg
     9961 gcgctgaaag tgctggaact gacagcgggt aaactggcgg ccttctatga ttccccgacc
    10021 ggattccgtc atggcccgaa atcgctggtc gataacgaaa cgctggtggt ggtatttgtc
    10081 tccagccacc cttacacccg tcagtatgat cttgatctgc tggcagaact ccgccgcgat
    10141 aaccaggcaa tgcgcgtaat cgccatcgcc gcggaaagca atgacgttat tactgccggt
    10201 ccacatatca tcctgccgcc gtcccgtcac tttatcgacg ttgagcaggc attttgcttc
    10261 ctgatgtacg cccagacatt tgcactgatg cagtcgctgc acatgggtaa tacaccggat
    10321 accccatcag ccagtggcac cgttaaccgc gtggtgcaag gcgtaatcat tcatccgtgg
    10381 caggcataag aggatcgcat tatgagcatt atctccacca aatatctgtt acaggacgcc
    10441 caggccaatg gctacgcggt gcctgccttt aacattcaca acgccgagac gatccaggcg
    10501 atcctcgaag tgtgcagtga aatgcgatca ccggtgatcc tcgccgggac gccggggacc
    10561 tttaagcata tcgcgctgga agagatctac gccctgtgta gcgcctattc cacaacctac
    10621 aacatgccgc tggcactgca tctcgaccac cacgaatcgc tggatgatat tcgccgtaaa
    10681 gtccacgcag gcgtgcgcag cgcgatgatc gacggcagtc acttcccgtt tgccgagaac
    10741 gtgaagctgg tgaaatcggt tgtcgacttc tgccactccc aggattgcag cgtggaagca
    10801 gaactgggcc gcctcggcgg tgttgaagat gacatgagcg ttgacgccga aagtgcattc
    10861 ctgaccgatc cacaagaagc taaacgcttt gtcgaactga ctggcgtcga cagcctggcg
    10921 gtagcgattg gtacggcgca cggcttatac agcaaaacgc cgaagattga tttccagcgg
    10981 ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc tgcatggtgc cagcgatgtc
    11041 ccggatgaat ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa cgttgccaca
    11101 gaattaaaaa tagccttcgc tggcgcggtt aaagcctggt ttgcggaaaa tccacagggt
    11161 aatgatcctc gttattatat gcgcgtcggt atggatgcga tgaaagaagt tgtcagaaat
    11221 aaaattaatg tctgtggttc agcgaatcga atttcagcat aatcattagt tttttatgat
    11281 ttatccc
//
LOCUS       DAZGAP010000169        11273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGAP010000169 DAZGAP010000000
VERSION     DAZGAP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.170"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..389)
                     /locus_tag="ACHLAM_004086"
     CDS             complement(<1..389)
                     /locus_tag="ACHLAM_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855538.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFACQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTT"
     gene            912..2840
                     /gene="thrS"
                     /locus_tag="ACHLAM_004087"
     CDS             912..2840
                     /gene="thrS"
                     /locus_tag="ACHLAM_004087"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO7855539.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2844..3386
                     /gene="infC"
                     /locus_tag="ACHLAM_004088"
     CDS             2844..3386
                     /gene="infC"
                     /locus_tag="ACHLAM_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO7855540.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3483..3680
                     /gene="rpmI"
                     /locus_tag="ACHLAM_004089"
     CDS             3483..3680
                     /gene="rpmI"
                     /locus_tag="ACHLAM_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO7855541.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3734..4090
                     /gene="rplT"
                     /locus_tag="ACHLAM_004090"
     CDS             3734..4090
                     /gene="rplT"
                     /locus_tag="ACHLAM_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO7855542.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4207..4331
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4213..4257
                     /gene="pheM"
                     /locus_tag="ACHLAM_004091"
     CDS             4213..4257
                     /gene="pheM"
                     /locus_tag="ACHLAM_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO7855543.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4541..5524
                     /gene="pheS"
                     /locus_tag="ACHLAM_004092"
     CDS             4541..5524
                     /gene="pheS"
                     /locus_tag="ACHLAM_004092"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO7855544.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5539..7926
                     /gene="pheT"
                     /locus_tag="ACHLAM_004093"
     CDS             5539..7926
                     /gene="pheT"
                     /locus_tag="ACHLAM_004093"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO7855545.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVIECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGAAIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7931..8230
                     /gene="ihfA"
                     /locus_tag="ACHLAM_004094"
     CDS             7931..8230
                     /gene="ihfA"
                     /locus_tag="ACHLAM_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO7855546.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8331..9311
                     /gene="btuC"
                     /locus_tag="ACHLAM_004095"
     CDS             8331..9311
                     /gene="btuC"
                     /locus_tag="ACHLAM_004095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO7855547.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9374..9925
                     /gene="btuE"
                     /locus_tag="ACHLAM_004096"
     CDS             9374..9925
                     /gene="btuE"
                     /locus_tag="ACHLAM_004096"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO7855548.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEESKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9925..10674
                     /gene="btuD"
                     /locus_tag="ACHLAM_004097"
     CDS             9925..10674
                     /gene="btuD"
                     /locus_tag="ACHLAM_004097"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO7855549.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQHQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLFLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10753..11217
                     /gene="nlpC"
                     /locus_tag="ACHLAM_004098"
     CDS             10753..11217
                     /gene="nlpC"
                     /locus_tag="ACHLAM_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO7855550.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         2826 a         2633 c         3047 g         2767 t
ORIGIN      
        1 gtagttaact gttcctgttc aatataatcc agtatgcttc gcaaatggtt agctttaata
       61 ggcgtggttt ccagtaataa ggaaatgatt tcttttttat agtgtaatgt aatgagtata
      121 ttttctgcat ggatgggtga agtggcaaag ttaatggtaa aaagggcagt ggaataacaa
      181 ttttgtgata tttttcgaca gtttgcggta aattcctgag taacacagta tgacagcatt
      241 gaacttaagt cagaggtatc aagtagggtg atgagttggc acgcaaattg attaataggc
      301 tctgatgaag gcgttggtat gtcgctatcg atgtgcattc tatgaggaga cttataatga
      361 gttctgataa taatgtcgtt ttgactcata atctattcgc cttatatatg tctggaggtt
      421 ttgaggatga tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa ttgctataag
      481 aattgtgtca cttttatcat tgtgatatga ccacttcgtc attaatctta tgatcgttta
      541 aatagtaccg ccaaattacg caacgaactt tgctggattc agtttatgct gctgcaaatc
      601 cgctcgcagt aaacctttca gacgctccgt gatgttatca gtggttcttt aagcgttttg
      661 ctggtgtact cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat
      721 ctcccttttt ttagtgttaa catcgttcaa ccgggtggaa ggtgagaggt taatgtcttt
      781 catttgtgta cctgattgcg aaccaattta gcatttgttg gctagatggt ttcgcaatga
      841 actgttaata aacaaatttt tctttgtatg tgatctttcg tgtgggtcac cactgcaaat
      901 aaggatataa aatgcctgtt ataactcttc ctgatggcag ccaacgccat tacgaccacg
      961 ctgtaagccc catggatgtt gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg
     1021 cagggcgcgt taatggcgaa ctggttgatg cttgcgatct gattgaaaac gacgcacaac
     1081 tgtcgatcat taccgccaaa gacgaagaag gtctggagat cattcgtcac tcctgtgcgc
     1141 acctgttagg gcacgcgatt aaacaacttt ggccgcatac caaaatggca atcggcccgg
     1201 ttattgacaa cggtttttat tacgacgttg atcttgaccg cacgttaacc caggaagatg
     1261 tcgaagcact cgagaagcgg atgcatgagc ttgctgagaa aaactacgac gtcattaaga
     1321 agaaagtcag ctggcacgaa gcgcgtgaaa ctttcgccaa ccgtggtgag agctacaaag
     1381 tctccattct tgacgaaaac atcgcccatg atgacaagcc aggtctgtac ttccatgaag
     1441 aatatgtcga tatgtgccgc ggtccgcacg taccgaacat gcgtttctgc catcatttca
     1501 aactaatgaa aacggcaggg gcttactggc gtggcgacag caacaacaaa atgttgcaac
     1561 gtatttacgg tacggcgtgg gcagacaaaa aagcgcttaa cgcttacctg cagcgcctgg
     1621 aagaagccgc gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg taccatatgc
     1681 aggaagaagc accgggtatg gtattctggc acaacgatgg ctggaccatc ttccgtgaac
     1741 tggaagtgtt tgttcgttct aaactgaaag agtaccagta tcaggaagtt aaaggtccgt
     1801 tcatgatgga ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa
     1861 tgttcaccac gtcttctgag aaccgtgaat actgcattaa gccgatgaac tgcccgggtc
     1921 acgtacaaat tttcaaccag gggctgaagt cgtatcgcga tttgccgctg cgtatggccg
     1981 agtttggtag ctgccaccgt aacgagccgt caggttcgct gcatggcctg atgcgtgtac
     2041 gtggttttac ccaggatgac gcgcatatct tctgtactga agaacaaatt cgcgatgaag
     2101 ttaacggatg tatccgttta gtctatgata tgtacagcac ttttggcttc gagaagatcg
     2161 tcgtcaaact ctccactcgt cctgaaaaac gtattggcag cgacgaaatg tgggatcgtg
     2221 ctgaggcgga cctggcagtt gcgctggaag aaaacaacat cccgtttgaa tatcaactgg
     2281 gtgaaggcgc tttctacggt ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg
     2341 catggcagtg cggtacagta cagctggact tctccttgcc gtctcgtctg agcgcctcct
     2401 atgtgggcga agacaacgag cgtaaggtac cggtaatgat tcaccgcgca attcttgggt
     2461 cgatggaacg tttcatcggt atcctgaccg aagaattcgc tggtttcttc ccgacctggc
     2521 ttgcgccggt tcaggttgtt atcatgaata ttaccgattc acagtctgat tacgttaacg
     2581 aattgacgca aaaactatca aatgcgggca ttcgtgttaa agcagacttg agaaatgaga
     2641 agattggctt taaaatccgc gagcacactt tgcgtcgcgt cccgtatatg ctggtctgtg
     2701 gtgataaaga ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg
     2761 gaagcatgga cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc agccgcagtc
     2821 ttaaacaatt ggaggaataa ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta
     2881 accgtatcaa tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc
     2941 agcttggtat tgtgagtctg agagaagctc tggagaaagc agaagaagcc ggagtagact
     3001 tagtcgagat cagccctaac gccgagccgc cggtttgtcg tataatggat tacggcaaat
     3061 tcctctatga aaagagcaag tcttctaagg aacagaagaa aaagcaaaaa gttatccagg
     3121 ttaaggaaat taaattccgt cctggtacag atgaaggcga ctatcaggta aaactccgca
     3181 gcctgattcg ctttctcgaa gagggtgata aagccaaaat cacgctgcgt ttccgcggtc
     3241 gtgagatggc gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc
     3301 aagaactggc agtggtcgaa tccttcccaa cgaagatcga aggccgccag atgatcatgg
     3361 tgctcgctcc taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg gagcctttgg
     3421 gcttcacagg cattgttcgc ctttgtttcg tttattaaca atgcgaagtg gaagttatta
     3481 aaatgccaaa aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag
     3541 gtggttttaa gcataagcac gctaacctgc gtcacattct gaccaaaaaa gcgaccaaac
     3601 gtaaacgtca cctgcgtccg aaagccatgg tttccaaagg cgatctgggc ctggtaatcg
     3661 cgtgcctgcc gtacgcataa gccgttaacg ttttttaact ttttaattag aatatagata
     3721 caggagagca catatggctc gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa
     3781 aattttgaaa caagctaaag gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt
     3841 ccaggctgtt atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac gtaagcgtca
     3901 gttccgtcaa ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta
     3961 cagcaaattc atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc
     4021 tgatatcgca gtattcgaca aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc
     4081 tctggcataa gccagttgaa agagggagct agtctccctc ttttcgtttc aacgccatca
     4141 aaacattgac ttttatcgcc gtagcctttt caataaaggt cttttgaaga gtaccaaaag
     4201 gtaacgcaag caatgaatgc tgctattttc cgcttctttt tttactttag cacctgactc
     4261 caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct cccagtggag
     4321 gctttttttg tatgcgcgtt ttgaaaaata aaaagcctgt ctcatcaggc gtaattgtcg
     4381 cagccagtct ggacacggcc agcgcggaga aaccggagcg tacttaagta cgtgaggatt
     4441 tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggatg ggctctaagt
     4501 ccaacgaacc agtgtcacca ctgacacaat gaggaaaaac atgtcacatc tcgcagaact
     4561 ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt
     4621 gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga
     4681 gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt
     4741 tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gctgcactga atgcgcgtct
     4801 ggcggcggaa acgattgatg tctccctgcc gggtcgtcgc attgaaaacg gcgggctgca
     4861 tccggtgacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt
     4921 ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg
     4981 tcaccacccg gcgcgcgctg accacgacac tttctggttt gacgctaccc gcctgctgcg
     5041 tacccagacc tctggcgtac agatccgcac catgaaagct cagcagccac cgattcgtat
     5101 catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca
     5161 tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga aaggcacgct
     5221 gcacgacttc ctgcgtaact tctttgagga agatttgcag attcgcttcc gtccttccta
     5281 cttcccgttt accgaacctt ctgcagaagt ggacgtcatg ggtaaaaacg gtaaatggct
     5341 ggaagtactg ggctgcggga tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc
     5401 ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacca tgttgcgtta
     5461 cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa
     5521 ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg
     5581 gcgattgata gcgatgcgct ggcaaatcaa atcactatgg cgggcctgga agttgacggt
     5641 gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtgat tgagtgtgcg
     5701 cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg cgatcgcctg
     5761 ctggacatcg tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagcgacc
     5821 attggtgctg ttctgccggg cgatttcaaa attaaagcgg cgaaactgcg tggcgaaccg
     5881 tctgaaggga tgctgtgctc cttttctgag ctgggaattt ctgacgatca cagcggcatt
     5941 atcgagctgc ctgcagatgc gccgattggc accgacatcc gcgaatacct gaaactcgat
     6001 gacaacacca tcgaaatcag cgtaacgcca aaccgtgccg attgcttagg cattatcggc
     6061 gtggcgcgtg atgttgccgt gctgaaccag ctgccgctgg ttgaaccgga aatcgttccg
     6121 gttggtgcgg ccatcgacga cacgctgccg attacagtcg aagcgccgga agcctgcccg
     6181 cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg
     6241 aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac
     6301 tatgtgctgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg cattgaaggc
     6361 ggcattgtgg tgcggatggc gaaagagggc gaaactctgg tgctgcttga tggcactgaa
     6421 gcgaagctga atgctgacac cctggtcatc gccgaccaca acaaggcgct ggcgatgggc
     6481 ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt cctgctggaa
     6541 tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact
     6601 gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa agcgatggaa
     6661 cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt aattgatatc
     6721 accaacgaag caacgctgcc gaagcgtgcc accattactt tacgtcgcag caaactggat
     6781 cgcctgatcg gccatcatat tgctgatgag caggtaactg acattctgcg tcgtctcggc
     6841 tgcgaagtga ccgaaggcaa agacgaatgg caggccgttg cgccgagctg gcgtttcgac
     6901 atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc
     6961 ccggatgagc cggtacaggc aagcctgatt atgggtactc accgcgaagc cgatctgtcg
     7021 ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc aggaagtgat cacctacagc
     7081 ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctgcca
     7141 agccctattt ccgttgaaat gtcagcaatg cgtctttccc tgtggaccgg cctgctggca
     7201 accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga aagtggtctg
     7261 cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat gttagccggt
     7321 gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc
     7381 tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt
     7441 gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg
     7501 aaaggtgaac gtattggttt tgttggggtt gttcatcctg aactggaacg taaactggat
     7561 cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg
     7621 cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc
     7681 gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag
     7741 gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag
     7801 agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt
     7861 gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg
     7921 gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg
     7981 gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc
     8041 tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa
     8101 gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg
     8161 cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa
     8221 agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct tttcactgtc
     8281 gaagagtcac cgtaaaatca acgccatgac acttcagcag aacggatacc atgctgacac
     8341 ttgcccgcca acaacagcga caaaatattc gctggttatt atgcctgtca gttttgatgc
     8401 tgctggcgct tctcttaagc ctttgcgccg gtgaactatg gatctcgcca ggtgactggt
     8461 tttctcctcg tggcgaactg ttcgtctggc aaattcgcct gccacgtacg ctggctgtat
     8521 tgctggttgg tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg tttgaaaatc
     8581 ctctggcaga acctggacta cttggcgtct ctaacggcgc aggcgtgggg cttatcgccg
     8641 cggtattgct tgggcaaggg caactcccca actgggcgct agggctgtgt gcgattgctg
     8701 gcgcgcttat catcacttta atactcttac gtttcgctcg tcgtcatctt tcgaccagtc
     8761 ggttattgct ggctggcgtt gcattaggga ttatctgtag cgcactaatg acgtgggcta
     8821 tctacttttc cacctctgtt gatttacgtc agctgatgta ctggatgatg ggcggttttg
     8881 gcggcgtaga ctggcggcaa agctggctga tgctggcatt gatccccgtg ttgttgtgga
     8941 tctgttgtca gtccaggccg atgaatatgt tagcacttgg cgagatctcg gcgcggcaac
     9001 tgggtttacc cctgtggttc tggcgcaatg tgttggtcgc agcgaccggc tggatggttg
     9061 gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg tctggtgatc ccacatattc
     9121 tccggctgtg tggtttaacc gatcatcgcg tattacttcc cggctgcgcg ctggcagggg
     9181 cgagcgcatt gctgctggcc gatattgtag cgcgcctggc attagctgcc gcagagctgc
     9241 ctattggcgt ggtcaccgca acgttgggtg cgccggtgtt tatctggtta ttgttaaaag
     9301 caggacgtta gccgcaaaaa gacggtctat gattaaaagc taagattttt actgaccaca
     9361 cccaggagaa acgatgcaag attccattct gacgaccgta gtgaaagata tcgacggtga
     9421 agtgaccacg ctggagaagt acgccggtaa tgtgctgttg attgtcaatg tcgcctcaaa
     9481 gtgtggctta acgccgcaat atgagcagtt ggagaatatt cagaaagcct gggccgatcg
     9541 tggttttgtg gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac cgggcagcga
     9601 tgaagagagt aaaacttact gtaccaccac atggggggtg acgttcccga tgttcagtaa
     9661 gattgaagtt aatggcgaag gacgccatcc gctgtatcaa aaattgattg ccgcagcgcc
     9721 gaccgcagtc gcgccggaag agagcggatt ctatgctcgt atggtcagca aaggtcgtgc
     9781 accgctgtac cctgatgata ttttatggaa ttttgagaaa tttttagttg gcagggacgg
     9841 attggtcatc cagcgttttt ccccggatat gacgccggaa gatcccattg tgatggaaag
     9901 cattaaactg gcgttggcaa aataatgtct attgtgatgc agttacagga tgttgcggaa
     9961 tctacccgcc tggggccgct ttctggcgag gttcgggctg gggagatcct gcacctggtg
    10021 gggccgaatg gcgcgggtaa gagtacctta ctggcgcgaa tggccggaat gaccagcggt
    10081 aagggaagca ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac aaaactcgcg
    10141 ctacatcgcg cctatctttc acaacaccag acgccaccgt ttgcaatgcc ggtctggcac
    10201 tacctgacac tgcatcagca cgataaaacg cgtaccgaac tactgaatga tgtcgcaggg
    10261 gcgctggctc ttgatgacaa actcggacgg agcaccaatc aactttccgg cggtgaatgg
    10321 caacgcgtac gtcttgctgc ggtggtgttg caaatcacac cacaagccaa tcccgcaggc
    10381 caattgctgt ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca aagtgcgtta
    10441 gacaaaattc tgagcgcact gtgtcagcaa ggactggcga ttgtgatgag cagtcacgat
    10501 ctcaaccaca ccttgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg aaaaatgctg
    10561 gccagtggac gcagggaaga ggtgctcacg ccgccaaatc tggcgcaggc ctatgggatg
    10621 aattttcgcc gtctggatat cgaaggtcac agaatgctga tttcgaccat ctgatttcat
    10681 taatatcttg aaaatcgggt aattacgacg ctaaattaat gcaagaataa aaaaacagag
    10741 gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag cactgtttct ggccgggtgt
    10801 agccaccata aagcaccgcc gccaaatgcc agactttctg attcgattac cgttattgcc
    10861 ggtttgaacg accagctaca aagctggcat ggcacgccgt atcgttatgg tggcatgacg
    10921 cggcgcggtg tggactgttc ggggtttgtg gttgtgacga tgcgcgatcg tttcgatttg
    10981 cagctgcccc gtgaaaccaa acaacaagcc tctatcggca cgcaaattga taaagacgag
    11041 ttgctgcctg gtgacctggt ctttttcaaa acgggctccg gacaaaatgg tttgcatgta
    11101 ggtatttatg ataccaacaa ccaatttatc cacgcctcta ccagcaaggg agtgatgcgt
    11161 tcctcacttg ataatgtcta ttggcagaaa aatttctggc aggcgagacg aatctagtat
    11221 gtcgaaaaat ggacggcgat gatacatcgc cgtccattta ctcctgaata cag
//
LOCUS       DAZGAP010000170        11208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGAP010000170 DAZGAP010000000
VERSION     DAZGAP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.171"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            159..1817
                     /gene="ybjD"
                     /locus_tag="ACHLAM_004099"
     CDS             159..1817
                     /gene="ybjD"
                     /locus_tag="ACHLAM_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO7855551.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1814..2770)
                     /gene="ybjX"
                     /locus_tag="ACHLAM_004100"
     CDS             complement(1814..2770)
                     /gene="ybjX"
                     /locus_tag="ACHLAM_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO7855552.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2921..4036
                     /gene="macA"
                     /locus_tag="ACHLAM_004101"
     CDS             2921..4036
                     /gene="macA"
                     /locus_tag="ACHLAM_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO7855553.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4033..5979
                     /gene="macB"
                     /locus_tag="ACHLAM_004102"
     CDS             4033..5979
                     /gene="macB"
                     /locus_tag="ACHLAM_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO7855554.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6052..6276)
                     /gene="cspD"
                     /locus_tag="ACHLAM_004103"
     CDS             complement(6052..6276)
                     /gene="cspD"
                     /locus_tag="ACHLAM_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO7855555.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6426..6500
                     /gene="yljB"
                     /locus_tag="ACHLAM_004104"
     CDS             6426..6500
                     /gene="yljB"
                     /locus_tag="ACHLAM_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO7855556.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6599..6919
                     /gene="clpS"
                     /locus_tag="ACHLAM_004105"
     CDS             6599..6919
                     /gene="clpS"
                     /locus_tag="ACHLAM_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO7855557.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6950..9226
                     /gene="clpA"
                     /locus_tag="ACHLAM_004106"
     CDS             6950..9226
                     /gene="clpA"
                     /locus_tag="ACHLAM_004106"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO7855558.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            9923..11131
                     /locus_tag="ACHLAM_004107"
     CDS             9923..11131
                     /locus_tag="ACHLAM_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7855559.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT         2763 a         2580 c         3122 g         2743 t
ORIGIN      
        1 tgtttttgca gacttatcag caagagggag tataacgcga ttattcgctc atttttcaga
       61 catttgccat gcttaaatgt gatgtcatca cgtattagca tggcctttcc cgttatactg
      121 ccagcgtaaa ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa
      181 ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct ggaacaaaac aacgtcctga
      241 ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc
      301 cagaatcaga tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca
      361 acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac
      421 atcgggttcg ccgctatcgg ccgctggaag cgtgctggac gccatgcacc gatggttatc
      481 accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc
      541 gcagttttct cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggctc
      601 gtcatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta
      661 ttcgtaacgg cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt
      721 tcctcgcccg tgaattatcc tcacatccgc aaaatctctc tgatgggcag attcgtcagg
      781 gactttccgc aatggtgcaa cttcttgagc attatttttc tgagcaggga gctggacagg
      841 cgcgatatcg tttaatgcgg cggcgagcca gcaatgagca acgaagctgg cgctatctgg
      901 atatcatcaa ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg
      961 gcctgtttgc tactttattg caggcaaaag gcacattgcg actggataaa gacgcccgtc
     1021 cattgttgct gatcgaagat ccagaaaccc gtttacaccc cattatgctt tcagttgcct
     1081 ggcatctgtt gaatcttctg cccttgcagc gcattgccac caccaactcg ggtgagttac
     1141 tttcgttaac gccggtagag catgtttgcc gactggtacg tgagtcctcg cgcgttgccg
     1201 cctggcgtct ggggccgagt ggcttgagta ccgaagatag ccgacgcata tcctttcaca
     1261 ttcgttttaa ccgtccgtca tcgctgtttg ctcgctgctg gctgctggtg gaaggggaaa
     1321 cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag
     1381 gaatcaaggt cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc
     1441 gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg
     1501 ctacggtacg cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt
     1561 taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc
     1621 gcgtggcgca aatcccggaa aatgtaccga tgaatctgcg caaaattatc tcgaaagcga
     1681 tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatggaggcc ggacgtcgtg
     1741 gtgtggactc cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc
     1801 gcggtcgcgc ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca
     1861 tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc
     1921 gtgctatctg tgccggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact
     1981 cccagaacgc gttgtaatcg gcatggatct tgccttcttt atcacgataa cgcaggctgc
     2041 ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa
     2101 acagacaggc cgcttccatc acaaggcgtt tgggaaatag cccgtggcag gctttcgtcg
     2161 cattctggat ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca
     2221 ttgttctttt ccccttatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt
     2281 cgctgttgcg gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca
     2341 gctcaagcgt gaactgctcg ccgtttttgc cttccagttt agccagttgc agcccggggg
     2401 tattcaaata taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt
     2461 aacgtaacgc ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgca
     2521 gacgcacagg cagtcgcggc tggcgcgtta acaacacatt gagattgggc cagtgggaaa
     2581 gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct
     2641 ggcgaaaact acggcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa
     2701 gaaaaagtga caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg
     2761 acatattcat gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc
     2821 aaacatcaat agtgtgatta ctaatgtaaa ttttagggtt ttgttgatat ttcgttgaag
     2881 ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt
     2941 gaagaagcgt tacgttattg cgctggtgat aatcatcgtc gggctgatta cgttatggag
     3001 aattcttaac gcacccgtgc cgacttatca gacgctgatt gtgcgccccg gtgatttaca
     3061 gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgatg tgggcgcgca
     3121 ggtcagcggt cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca
     3181 gcttttaggg gttattgatc ctgaacaggc tgaaaaccag attaaggagg tggaagcaac
     3241 gctgatggag ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt
     3301 gacgtattcc cgtcagcaac gtcttgcaca aacgcaagcg gtttcacagc aggatctcga
     3361 taacgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat
     3421 caagcgcaat caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgcatcgt
     3481 tgccccgatg gccggggaag tcacgcaaat caccactctg caaggccaga cggtgattgc
     3541 cgcacaacaa gcaccgaaca ttctgacgct ggcagatatg agcaccatgc tggtaaaagc
     3601 gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt
     3661 gcttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga
     3721 aaagattaac gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt
     3781 actgcggctg gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgct
     3841 gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt
     3901 gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga
     3961 tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc
     4021 aggagctgcg caatgacacc cttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc
     4081 ggtgatgagc aggttgaggt gctgaagggc atcagcctcg atatttatgc tggtgagatg
     4141 gtggcgattg tcggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc
     4201 ctggataagg ccaccagcgg cacctatcgc gtagccggtc aggatgttgc cacgctggac
     4261 gccgatgcgc tggcgcaact acgccgcgag catttcggct ttattttcca gcgttaccat
     4321 ttgctttcgc atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt
     4381 gagcggaaac agcggctgct tcgcgcccag gagttgctgc aacgactggg gctggaagac
     4441 cgtacagagt attacccggc acagctttcg ggtggtcagc aacagcgcgt cagcatagcg
     4501 cgggcattga tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac
     4561 agccattctg gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg
     4621 gtgattatcg ttacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt
     4681 cgcgacggtg aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg
     4741 acggaacctg ttgtcaacac ggcgtctggc tggcggcagt ttgtcagcgg ttttaacgag
     4801 gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg
     4861 ctggggatta ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc
     4921 aaacaaatgg tgctggcgga tattcgttct attggtacga atactattga tgtctatccc
     4981 gggaaagatt ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta
     5041 atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg
     5101 cgcctgcgtt ataacaatgt tgatgtggct gccagtgcca atggcgtgag cggcgattat
     5161 tttaatgtct atggcatgac cttcagtgaa gggaacacct ttaatcagga gcagctgaac
     5221 ggtcgtgcgc aggtggtggt gctcgacagt aatactcgcc gccagctttt cccccataaa
     5281 gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg
     5341 gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac
     5401 agcacgatgt ccgggcgagt tatgggccag tcgtggctga actccattac tgtcagggtg
     5461 aaagaaggct ttgacagcgc tgaggcggaa cagcaactca cgcgtttact ttcactgcgc
     5521 cacggtaaga aggatttctt tacctggaac atggacggcg tcttgaaaac cgttgaaaag
     5581 accacacgta ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc
     5641 ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc
     5701 attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gattgaagcc
     5761 gtactggttt gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc
     5821 accttacagc ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg
     5881 gcgtttctct gctcgacggt caccgggatt ttatttggct ggttacccgc acgaaatgcg
     5941 gcacgactgg atccagtaga tgctctggca cgagagtaat ttttgagata aaaatgccag
     6001 ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac
     6061 tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg
     6121 gacatcaaac tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt
     6181 ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt
     6241 ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt
     6301 atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact
     6361 ctagaaatac aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg
     6421 caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg
     6481 ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa
     6541 gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat
     6601 gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc
     6661 gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattata ctccgatgga
     6721 gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt
     6781 gatgctcgct gttcactacc aggggaaggc catttgtgga gtctttaccg ctgaggttgc
     6841 agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg
     6901 tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca
     6961 agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt
     7021 tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct
     7081 ggaagcgtgc tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca
     7141 aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt
     7201 tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac
     7261 cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg catatctgtt
     7321 gcgcaaacat gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa
     7381 agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg
     7441 tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc gagtgggcgg
     7501 aatcgaccca ctgattggtc gtgagaagga gctggagcgt gctattcagg ttctctgccg
     7561 tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc
     7621 ggaggggctt gcatggcgaa ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac
     7681 gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga
     7741 aaaacgtttt aaagcgttgc tcaagcagct ggagcaagac actaacagca tcctgtttat
     7801 tgatgagatc cacaccatta tcggtgcggg tgcagcgtct ggtggccagg tcgatgcagc
     7861 taacctgatc aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta
     7921 tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat
     7981 tgatattact gaaccgtcaa tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa
     8041 gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcag cggtagagct
     8101 ggcggtgaaa tacattaacg atcgtcatct gccggataaa gccattgacg ttatcgacga
     8161 agcgggcgct cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc
     8221 ggatattgag tccgtggtgg cccgtattgc gcgcattcca gagaagagtg tttcgcagag
     8281 tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggtcagga
     8341 taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga
     8401 acataaaccg gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt
     8461 gacggtacag ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta
     8521 tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga
     8581 tcagggcggt ttgctgactg atgcggtcat caagcatcca catgcggtgc tgctgctgga
     8641 cgaaatcgag aaagcgcacc cggacgtgtt caatattctg ttgcaggtga tggacaacgg
     8701 tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtat tggtgatgac
     8761 caccaacgct ggggtacgag aaactgagcg taaatcgatt ggtcttatcc accaggacaa
     8821 cagtaccgat gcgatggaag agatcaagaa gatctttacg ccggagtttc gtaaccgtct
     8881 cgacaacatt atctggttcg atcatctctc caccgacgtg atccatcagg tagtggataa
     8941 attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca
     9001 ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat
     9061 ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgt tgtttggttc
     9121 gctggtggac ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta
     9181 tggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatcta attgttaggt
     9241 aggttggtga agtccgtaat ctcgaaagag gttacggact ttttgtttat ggggtggagg
     9301 aggttctgac cctttttttg atgatgatgg taatttgttg ataattagtg ctgcgggaag
     9361 gtaaggataa aaaagggtgg tagcaggaaa atgagatggt tttgctttat taacaacggg
     9421 ctagacgtgt agtatttgag ttcactgccg tacaggcagc ttagaaagcc aaccgaaaga
     9481 aaaactcgat ttttataccg ttcactgccg tacagacaga taaaatgcga aaaaaaaaac
     9541 gtactttcgt acgagctctt ctttgaatat ggcggtgagg gggggattga ctcgcttcgc
     9601 tcgccctgcg ggcagcccac tcactgtgtt cgtggtctgt ccaactggct gcgccagttg
     9661 tcgaaccccg gtcggggctt ctcatccccc ctggagtgca atatgcgaaa aaaaagctcg
     9721 cactttcgta cgagctcttc tttgaatatg gcggtgaggg ggaccgcctg aaataaatct
     9781 gacttaatcc gggagtgtta tataacttca ttataaacaa tgcattacaa tttttgctgt
     9841 ccgatgttat ctaatgtatt cttagtgaag tgagagacta atggcatact ggatggcata
     9901 ccagagtaac aggaattagc gtatggcagt attgacggat actaaagcaa ggcatatcaa
     9961 acctgatgac aaaccattgc cccatggggg aattacagga ctgactcttc atccttcttc
    10021 agtaaagggg cgggggaaat gggtttttcg ttatgtaagt ccggtgacac aaaaaaggcg
    10081 taatgctgga ttgggtactt accctgaggt cagtattgct gaagctgcac gtactgcccg
    10141 gataatgcga gagcaacttg ctgcaggtga tgatcctctg gagattaaaa aggctgaagc
    10201 tgaaaaagtt gttatcccaa catttgccga tgcagcccgg cgtgtacatg cagaattgtc
    10261 accgggatgg gaaaacccaa agcatgtcag gcagtggttg tcgacgcttg aaaattacgt
    10321 gtttcctcaa ctgggagcaa aaacgctgga tacgattacg gctgcggacg tggcagagac
    10381 actgcgtcca gtctggttga ccttatcaga aacggcaagc cgggttaaac agcgcattca
    10441 tgttgttatg cagtggggct gggcgcatgg tttttgtgtg gcgaatcctg ttgatgtggt
    10501 tgatcatttg cttccacagc aatcaagagg acgtgatgaa caccagccgg caatgccctg
    10561 gaggcagtta ccgctttttg tggcgaccag tgtgtataca gatgaacctt ataatgttac
    10621 ccgggcactg ttattaatgg tgatactgac agcaacccgc tcgggcgaag caaggggaat
    10681 gcgctgggct gaaattgatt ttcataagcg gatatggacg atacccgcag aaagaatgaa
    10741 agccaggata cagcatcgtg ttcctttatc ccgacaggcc attcacgttc tggaaaatat
    10801 acgtggtctg catgacgaac tggtgtttcc ttctcccaga aagcagcaga tcctttcaga
    10861 tatggtgttg acgagttttc tacgtaaaaa gaaggccatc agtgatatac ccggacgagt
    10921 ggctacagca catggttttc gttcaacatt cagggactgg tgtagcgaac agggatattc
    10981 gcgggatttg gcggaaaggg cgcttgccca cacgctgaaa aataaggttg aggcggcata
    11041 tcaccggact gatctgctgg atcagcgtat accgatgatg caggcatggg cggattatgt
    11101 gatgtctcag attatggaaa accagcgatg aaatcacatc tcacagattt gcagatgtcg
    11161 tctgtaatca cgaaaaatat acttattgag ttataatgct tgtttgtc
//
LOCUS       DAZGAP010000171        11204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGAP010000171 DAZGAP010000000
VERSION     DAZGAP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.172"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..1764
                     /locus_tag="ACHLAM_004108"
     CDS             208..1764
                     /locus_tag="ACHLAM_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO7855560.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGWIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            1814..2533
                     /locus_tag="ACHLAM_004109"
     CDS             1814..2533
                     /locus_tag="ACHLAM_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO7855561.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            2544..3959
                     /locus_tag="ACHLAM_004110"
     CDS             2544..3959
                     /locus_tag="ACHLAM_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO7855562.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            3964..4659
                     /locus_tag="ACHLAM_004111"
     CDS             3964..4659
                     /locus_tag="ACHLAM_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO7855563.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
     gene            complement(4839..7955)
                     /locus_tag="ACHLAM_004112"
     CDS             complement(4839..7955)
                     /locus_tag="ACHLAM_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO7855564.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQLHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPIFPENASGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAAN
                     KSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQ
                     MNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIESEKEKKDFVKLFGEYLRAENVLQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGHL"
     gene            complement(8075..9289)
                     /locus_tag="ACHLAM_004113"
                     /pseudo
     CDS             complement(8075..9289)
                     /locus_tag="ACHLAM_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(9286..10842)
                     /locus_tag="ACHLAM_004114"
     CDS             complement(9286..10842)
                     /locus_tag="ACHLAM_004114"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO7855565.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSHMENGDDSICYAKLDDNVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCGVQK"
     gene            11106..>11204
                     /locus_tag="ACHLAM_004115"
     CDS             11106..>11204
                     /locus_tag="ACHLAM_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7855566.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLT"
BASE COUNT         2874 a         2500 c         2581 g         3249 t
ORIGIN      
        1 taacaataaa acgattcagc aatcattttt cataacaaaa acaaattata acccccttcc
       61 ctgtttgtgg acgagactcg ttctctccct gcgagacaag cgtaaattgg tcacaccgac
      121 ggtcgttgcc tgaaaattac aaattctcgc ctgacagggc aataacaaca gtaatgatgc
      181 tgaatttgtt ttcgttgagg aaatgacatg caccaatacc tgcttttttt actgggactt
      241 accccacttc tggcaatgat cattttgatt ctgaaagtca aaatgcccat acattatgcc
      301 gtactcagca cactctttat tacactgatt cttgcaactt tgttctggca cacaccagtt
      361 caggatctgg gttctgctgt tgtatatggc gcaatcaaag ggctgtggcc gatagtgata
      421 gtcatacttg ctgccattta cagctataac ctgatgaatg cgacaaaaag catgaacatt
      481 ctgcgaaatg ttctggccgc tgtcagtgat gataaacgta tccaggtgct tcttatctca
      541 tggtgttttg gtggatttct tgaggcggcg gccgggtacg gtacagctgt agcaatcccc
      601 attggtatac tcattgcact gggctttaac cctcttaaag ccgcaattgc atcattggtt
      661 gctaatacag tacctaccgc gtttggtgct gtcggcatac ctgtatcagt acttgctgaa
      721 caggtaaatt tacccgtaac aaccctcagc gggacgatta ttcttcagct ggcagcgttc
      781 aacattctgc ttcccttcgt tatagtcgct attattgggg aaggcctgaa ggctatacga
      841 ggcgttgggt tcatgacctt aatgtgcggt gtgagcacat taatcccaca gtacctggtg
      901 gctgtttatc ttggcgcaga acttcctgct ttcgcgggaa gtctggtcag cctgatcgtg
      961 atagtcttta tggcaaaaaa ccgtaaagaa aaaacagctc ctgaatttct tattgagact
     1021 tcagcagata gcgccagcgc actttcatac tctcgtgggg cgttgttacg tgcgtgctca
     1081 atctacatcc tgatttttct gttcattctt ctttgctctc cactgttccc gacggtaaaa
     1141 agtacagtgt ctcagattgc ctcgtcactc agcttctctc tgagtaacgg cagcaatctg
     1201 tcgatgaaga ttgaatggat cgcaaccccc ggtgttctga ttatcattgc cagtgttatt
     1261 ggcggctgga tacagaaagc atccctgtca tgcctgttaa atgtatttgt cagtacggta
     1321 aaacagctca aaaactccat tattgccatt tctgcgattg ttgctatggc aacggtaatg
     1381 gatgtttgtg ggttgattgg cactctggca cagagccttg tagatctcac tggtggggcc
     1441 tacctcttca ttgctccggt tatcggcgct cttggtacat ttgttactgg cagtgacaca
     1501 aactccaaca ttctgtttgg taaattgcag accactgcgg ctgaaaaatt gcatgtggac
     1561 ccactctggc tggctgccgc taacacatct ggcgcaacag gcggcaaaat gatttctcct
     1621 cagagtattg ctatcgctgt ttcagccacc cgtatggatg gtcaggggag cgtaatcatg
     1681 tcagggacac tgaagtactg ctttgcttat attgttattc ttggactgaa agtaggcctt
     1741 gtctaccacc tgttcatggc ctgagaaaaa ataacaaacg gacgtggaat attaatcaac
     1801 aggagaaata tctgtgaatg ttaacttttt cgtcacctgc attggtgatg catttaaatc
     1861 ccgtatggca caaaacagcg tgctgttact ggagcaattg ggttgcaatg tccatttccc
     1921 cgaaaaacag ggatgttgcg gtcagccagc gataaatggc ggttatgttc gtgatgccat
     1981 tcctggtatg aaaaatctca tatcttcgct ggaagagaat gactggccgg tagtatctcc
     2041 tgccgggtca tgcacttaca cgataaaaaa ttacgctcgc tatctcagtg aagaacctga
     2101 gtgggcggca cgagctgaaa aagtagctga acgcctgcat gatttgacgt catttatagt
     2161 aaatcatctt ggcatcacag atgttggggc aagactcaat ggtcgcgcag tgtaccatcc
     2221 ttcatgcagc ctttcccgta aactgggtgt aacccgagaa ccgcttttgt tgcttgagaa
     2281 tgtacgggga cttgagctgc tgccgtttca cgctcaggaa acgtgctgcg ggtttggcgg
     2341 cacgttctct gtaaaaatgg cggagatctc tggtgaaatg gtgaaagaga aagtcgggca
     2401 tattatggat gttaaacctg attttcttat cggggctgac gccagttgcc tgctcaacat
     2461 cggcggtcgg cttagccgtg aagggcatcc tgtaaaagtg atgcatattg ctgaagtcct
     2521 gatgcacaga tgaggtaaac agaatgtctt tgaaaacaag tgatatcagg tttaaagagc
     2581 gtatccgtat tcagattcag gacccgatca tgcgaaaagc tgtggctaac gcacaggagc
     2641 gcatagggac taatcgacag aaaatggttg atgaactggg gcactgggaa gaatggcgtt
     2701 cacttgctga acagattcgt cggcatgttc tggaaaatct tgatgcttat ttgtatcaat
     2761 tgtccgaaaa agttactgcg aatggcggac atgtatattt tgcccgtacc agagaggagg
     2821 caaccagtta catactgaag gtggcgaaag aaaaaaacgc caggaaggtt gttaaatcca
     2881 aatcaatggt gactgaggaa attggtatga acggcgttct tcagaaagcc gggataccag
     2941 tgatagaaac agatcttggt gaatatattc ttcagctggc gcatgagccg ccatcgcatg
     3001 ttgttgtgcc ggcaatacat aaagatcgcc atcaaattcg cagagttttg catgaacatc
     3061 tggggtatga cggccctgaa actccagaag caatgacatt gttcatccgt gagaaaatca
     3121 gacaggattt tctgagtgct gaagttggcg taacagggtg taactttgcc gttgcagaaa
     3181 caggctcagt ttgccttgtg acaaacgaag gtaacgcacg tatgtgcacc acgttaccta
     3241 aaacacatat tgctgtgatg ggtatggagc gtattgcacc aacattccgg gaggtggatg
     3301 ttcttatcac catgcttgca aggagtgctg tgggagcaag actgacaggt tataacacct
     3361 ggcttacagg tccaagagaa gaaggacatg ttgacggccc ggaagagttc catctcgtca
     3421 ttgttgacaa tgggcgttca gaggttctcg gctcagaatt cagcgatgta ttgcgctgta
     3481 ttcgctgcgg tgcctgtatt aatacctgtc ccgcttatcg ccagattggt ggacatggct
     3541 atggctcaat ctacccgggc ccaatcggag cagttatttc cccgttgctt ggtggatatg
     3601 aagactttaa ggaattacct tatgcctgct cattgtgcac tgcctgcaat ggggtatgtc
     3661 cggtaaaaat acctctggca caactaatac aaaaacatcg gcaggttatg gcagagtccg
     3721 aaataacacc atcaacagaa cgccgtataa ccagaatatt caattacgta aacagtcatc
     3781 cggcattgtg gaaagttggc atgattgctg gcgcgaaaac agccagcctg tgcatcagga
     3841 acggaaaaac gccgattagc ccaggtgcta ttaaagaatg gtctgtggcc agagacttac
     3901 cttctcctga tggagaaagc ttccgtagtt ggttcaaaag acataaaaaa ggagggtaac
     3961 tttatggaaa acagagagga atttctcgtt gctatcgcca gaaagcttgg caggccagta
     4021 agacgtattc ccgaagcaat gccggagcct gtaagtacgc tggctacgac acgtttaacc
     4081 gagttgacaa tggaccaacg ctgtgaggct tttattaaat ttgcctcaga agtgatgctt
     4141 gcgaagtgcg ttctggtttc gcctgaagac gcaccatcaa aagcattaga catctgttgg
     4201 acgttcggtt ccgggccagt cattatcagc aacgatcaac gactgtttga tacggggatt
     4261 gcaccattgc tacaaaaaga gatgggcgct gtcctgtgga ccccggagaa gggtgaggaa
     4321 aatattcatc ttgcagaaaa agcaaaaaca ggcgtcgttt tcgctgagta tggactgaca
     4381 gaatctggtg gtgttgtttt attctccgcc cctgaacgag ggcgttcagt aagtctttta
     4441 cctgaagcgt ctgtgtttgt tttacgcaaa agtaccattc ttcccagagt ggctcagctt
     4501 gcgcaaaaac ttcatgatat ggctcaggaa ggacaacgta tgccttcctg tatcaatctg
     4561 attggaggcc caagttctac tgcagatata gaactgatca aagttgttgg tgtgcatgga
     4621 cctgtacatg caacttactt gattattgat gactgctaat acattttagt atgtatatat
     4681 gattaacagc tccgtttagc tgagctgtta atcattcatt tagttgtatt agtcgtgact
     4741 tgagcgtaag cgtcaatacg gactccgtta acgccttaca atagcagtca gtgttcatgc
     4801 ctgtttattg aggcaagggg gagtctgggc gcctgtaact aaagatgccc ccccacgcct
     4861 ttaaacttct caataaatgc gctaatcttc tgaaaaacgg tctgtttttt cgttttatat
     4921 tgcgggttta acggactaag ttttggtaat gtctcgttta attctgtgcc attttcggtg
     4981 gcgtattcgc gttttaacga cgtgcgaata tagcgcctgg ccgcttcttc attaagattt
     5041 tcttctttga tcaatgcatc tgcttcacgt tgttgttcgc gttgagcaaa ggtaaagaac
     5101 gtatcaatga tgctggcttt gtccggcaaa tcatcaaggt tcgtttgctg aataaaatcg
     5161 accaccaggc cttctttagc acggttcccc agacttgaac gaattaatcg tttgacctct
     5221 acggtcattt cgtccttact tttgttttgt ctgctgtgtt cgaaaatcag gccaaggata
     5281 taatccaggt ttatttcctg agacttcagc aaatcgacct caaaaaccac gtcatcccag
     5341 tcagtggttg atttttcttt tttgtcagct tctttttcgc ggtgctgcca gtcacgaata
     5401 tcgttatagg cagaacgata atcctgaatc ttgcgttcag cagggagacg aatcatttgc
     5461 aattcagcga acttttcatc atccacataa tgttctgctt tgaatttttc taccgcaaca
     5521 ggatcgctga gatcgatttg ttgcagggct ttcagcgtgg caaattcatc atagttttgc
     5581 aggacgttct cggcacgcag gtattcacca aacagtttaa cgaagtcttt tttctctttt
     5641 tcgctttcaa tactggcagg gtcagggaac ctttgttcca gttctgaaac tactgccata
     5701 aagccgcgtt tagcttcgcc agtggcagca tctgtaaagc cttccatata ctctgcataa
     5761 ctcttttcca gcaccacatt tctggtgttt ttatcaccaa aaagtgtgat ggcatcgatg
     5821 gttgcctgct ccaggttacg gaatgtgacg atgttaccga aggttttcgt ggcgtcataa
     5881 atgcggttgg tacgggaaaa cgcctgcatc agaccgtgaa aacgcaggtt tttatcgacg
     5941 aacagcgtgt tcagtgttgg agcatcgaag ccggttaaaa acatccccac gacgattaac
     6001 aggtcaacat cctgattttt aacccgctga gccaggtccc gatagtagtt ctgaaaatcc
     6061 ttgctatcag tactgaaatt catttgaaaa tggtcgttat attcacgaat tgcagcatcc
     6121 agaaactctt tagcactgct gtccattgcg ccggtatcaa aagtttcatc ggaaatttca
     6181 ccaatggcat tttgttcttc attggcggca aaggagaata ttgtcgcaat acgcagtggt
     6241 ttataatttg cagatttatt agcggcttcc tcctgtaagc gtttaaacgt cgcgtaatag
     6301 actttcgcag catccacgct gcttactgcc agcatggcat taaaacctct ggaacccggg
     6361 aaagtgcggt gggtcttctg gcggaagtta ttcaggatat attgcgtgat ttcctgaata
     6421 cgcatgggat gaagaaacgc ctgctgattt tcagccgcac tcagtttttt ctcgtcagtt
     6481 tctgtctcta aagacttaaa ctgtggccgc acatcgttgt agtccacctt gaatttgagc
     6541 actttttcgt cacgaatcgc atcggtaatc acatacgaat gcaattcacg accaaatacg
     6601 ctggcggtcg tttctgaacc tgaggcgttt tccgggaaaa taggtgtgcc ggtaaaacca
     6661 aactgataat agcgtctgaa tttcttcttc agatttttct gcgcttctcc aaactggctg
     6721 cggtggcatt catcaaatat aaacaccact tgctgattgt atacaggcag gtcgctttct
     6781 gctttcatca ggttattaag tttctgaata gtggtgacga taattttgtt atcgtcctta
     6841 tccagatttc gtttaaggcc tgcggtattt tccgagccgt taacactatc tggcgaaaaa
     6901 cgctgatatt ccttcatggt ctggtaatcg aggtctttcc tgtcgaccac aaagaagact
     6961 ttatcaataa agtccagttc tgttgccaga cgcgcggctt taaagctggt cagtgtttta
     7021 ccggaacccg tggtgtgcca gatataccca ccgctttccg gttttgacca gttcttcgct
     7081 gtaaaggagc tcttaatttt ccacagaatg cgctcggtgg cagcaatctg gtacggtcgc
     7141 atcaccagta gcgtctgact gctgtcaaaa acgctgtagt tcaccagaac attaagcagg
     7201 gtatgtttct ggaaaaaggt ggcggtaaaa tctttgaggt ctttaatcag cgtgttgtct
     7261 gatttcgccc agttcatggt gaagtcaaaa ctgtttttat cgcgctttgt cgtgttggca
     7321 aagtaacggg tatcggtacc gttggaaatg acgaacagtt gcagatactt aaacagggaa
     7381 ttttcgctgt taaaactctc cttgctgtaa cgatgtaact ggttgaaagc ctcacgaatc
     7441 gccacgccac gctttttcag ttcgatttgc accagcggca aaccattaac caggatagtg
     7501 acgtcataac ggttactgtg agaacctgcc tgttcaaact gccggataat ctgcactttg
     7561 ttgcgcatga gattcttttt atctatcaga tagatattct caagacgccc gttgtcaaaa
     7621 ataaagtcac agatatagtc gatatggatt ttacgggtct tatccagaat gccatcgcca
     7681 gggttgtcca gatactgctc cgtgaaacgc cgccattcgc tgtcattaaa caccacgcca
     7741 ttgaggctct gaagctgttc ccggacatta gccagcatcg ccgactgtga ttttacggaa
     7801 ataaattcat agccctgatt ctgcaggtcc tgaatcagtt cacgttccag gtccgattca
     7861 ctttggtagc tgtcgcctgt ttgctcagcc ttgatgtact tatcaagaac gataaagtta
     7921 ctggtttcag caatggtgtg tgtctgaaga atcatagcgc atcctttgtg ccgtctggcg
     7981 gggccggaag ggagttaagg gtgacttccg gcgtgtaaaa atggtttata tactgaccgg
     8041 atgttaaggt ggcccggtcg gtagcaattg tcaattagtt actgacggtt tcaggtttcg
     8101 ggaaactgaa cagtaaatca cgatagtact cgtattgttt ctggcgcaac tcgatttcac
     8161 gcgggagacc ttcggtgatg gagttggtca gggtgtcgaa tttatcgagt atttcgacaa
     8221 tgcgagcttg ttcttctgaa ggtggtacag gtattgggaa ttttaaaaac attggtcgac
     8281 gaagtgaggt aaccgatgaa cttactgatg ctttttgaat ataggtaaaa aatatgctgt
     8341 tatatagtga tatacaaatc gcggattcat aaatgcattt gggacaatac gatatgctct
     8401 ctgatgcaaa gcatatttac ctttagcgta atgaaatacc ttccccaccc cggcaccatc
     8461 tccagccgta atgattgctg tttcatcaaa atcaaataca tttaacttta atggttcacg
     8521 tccacgtgca taaaaaatat atttgccatg ttctatagca tcttgcgtat catggttacc
     8581 tgttcctata acagccactt cccccaaagt cttccactca atccgaatac catccatggc
     8641 gtccggaagg atgtaattac cccccccgca atatctttga aattcaacaa cttatctcga
     8701 tagtagttgt attgtttttt acgcatgtta agctctgctg tcagctctgc tgtcagctct
     8761 gctgtcagct ctgctgtcag ggcgctgaat ttgtccagaa ttcgcacgat ttcagactgg
     8821 atggcaaggg atttttccgg attatccggg caggggattg gtacaggtat attttcaacc
     8881 tgatgactca ttaacttggg attcccattc ctgagtaaac atgttttttg gcctctattg
     8941 ttagccagta aaacagaaat ttgtagatta atggatattt actttttaat gtaattaggc
     9001 cgcaaacatt agttattgaa aatttacctg ttcgatagaa cacagtgcct gcatttgcgc
     9061 catctgttgt ccagcttata tattcgccat caaaatcgaa ggtattgata gaacctattt
     9121 tcccattatt tgcagtttga gagctataga caggatatgg gccaaaattt tcagttaaat
     9181 atccctttga cataactctg cctcttctta aggctgagac ttcccccaat ggcaaccact
     9241 caacctcaac cccatccagc aatttttcca gataactcaa ctcgctcatt tctgcacccc
     9301 gcagccttca atttcagcca caatcgcatc aatatcttta cgcaactggt cgattttgct
     9361 gaccgtggtt ttcagctcag cattcagctc agcaatattg ataatttcac gagtatcttt
     9421 cgcttccaca tagctgctca ccgacaggtt atagtcatta gcgacaacgg tctcaaacgc
     9481 gactgatttc gccagatggg caacatcttc cttgctggca aatacctgca taatctgttc
     9541 gatatgagca tcggtcagaa tgttgttgtt agcctctttt ttgaacagtt cgctggcatc
     9601 aataaactga actctggtat ccgttttatg tttagacagc accagtatgt ttacggcaat
     9661 ggtggtgcca aagaacaggt tcggtgccag tgaaatcacg gtttcgacat agttattgtc
     9721 gaccagatac tgacggattt tctgctctgc gccgccacgg taaaaaatac ccgggaagca
     9781 gacaatcgca gcgcgacctc tggccgaaag atagttcagc gcatgtaata caaacgcaaa
     9841 gtcagctttg gatttggggg ccagaacgcc agccggggca aaacgctcat cgttaatcag
     9901 tgtcgggtca tcgctgccag tccatttcac cgaatacggc gggttagaaa cgatggcatc
     9961 aaacggtttt tcatctctga agtgcggctc agtcagcgta ttgcccagct tgatatcaaa
    10021 cttgtcgtag ttgatgttgt gcaaaaacat gttcatacgc gccaggttat aggtcgtatg
    10081 gttgatttcc tgaccaaaaa agccttcttc gatgatatgg tcatcaaact gctttttcgc
    10141 ctgcaacaac agcgaaccgg aacccgctgc cgggtcgtag attttgttaa cgtgggtctg
    10201 cccgtgcata gccagttgtg caatcagctt agagacgtgc tgtggtgtaa agaactcgcc
    10261 gcctgactta ccggcatttg cggcatagtt agaaataagg aattcatagg catcgccaaa
    10321 caggtcaatc tgatgtttgt tgaagtcacc aaggtttaac ccttcaaccc ctttcagaac
    10381 agcagccagg cgggcatttt tatccttaac ggtgttaccc aggcggttac tggtggtatc
    10441 gaaatcagca aacaaaccct tgatgtcagc ttctgaagga taaccgtaag cagaactttc
    10501 gatagcaacg aagatgctgt ttaaatctgc attcagtctg tcattggtat ttgctttcgc
    10561 agctacgttg cagaaaagct gactggggta gatgaagtag cctttagtcc tgatggcatc
    10621 gtctttaatg tcatcagtaa ttacgttgtc atccagtttt gcataacaga tgctgtcatc
    10681 accattttcc atatggctgg aaaaattttc gctgataaaa cggtagaaaa gtgcgcccag
    10741 aacgtattgc ttaaaatccc atccatcaac agaacctctg acatcattgg caatttgcca
    10801 tatctggcgg tgcagttctg ctcgttgttg aatacttgtc atttttaatc cattttcctg
    10861 gtctggacct gaatcattgg gggcaattct acagcaatct tgtggttcag gcagccccgg
    10921 agtaagtcgg tgtacattgt gagatacctg gggtattcac gacaaggagg aggaacggaa
    10981 tactgccttt cctttgcagt gcaccaggta cattctaaat aatttcagac agatattatc
    11041 gttgtgagtc ccccgcctcc tttccggtgt gcggggtttt gtcttttttc accgggaata
    11101 catgtatgaa tccgtctgat gccattgagg caattgaaaa accgctctcc tccctgcctt
    11161 actcactttc ccgtcacatc ctggaacatc tgcgcaaact cacc
//
LOCUS       DAZGAP010000172        11007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGAP010000172 DAZGAP010000000
VERSION     DAZGAP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.173"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..280)
                     /locus_tag="ACHLAM_004116"
     CDS             complement(<1..280)
                     /locus_tag="ACHLAM_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855567.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVCLYIGTLVKNIFIRIFAFSIWALSA"
     gene            540..1361
                     /locus_tag="ACHLAM_004117"
     CDS             540..1361
                     /locus_tag="ACHLAM_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO7855568.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            1451..2653
                     /locus_tag="ACHLAM_004118"
     CDS             1451..2653
                     /locus_tag="ACHLAM_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO7855569.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLLPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            2793..2963
                     /locus_tag="ACHLAM_004119"
     CDS             2793..2963
                     /locus_tag="ACHLAM_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855570.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVFQPFSKNTNQ"
     gene            3012..3464
                     /locus_tag="ACHLAM_004120"
     CDS             3012..3464
                     /locus_tag="ACHLAM_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO7855571.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            3461..4297
                     /locus_tag="ACHLAM_004121"
     CDS             3461..4297
                     /locus_tag="ACHLAM_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO7855572.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            4281..5429
                     /gene="traJ"
                     /locus_tag="ACHLAM_004122"
     CDS             4281..5429
                     /gene="traJ"
                     /locus_tag="ACHLAM_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO7855573.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            5426..5716
                     /locus_tag="ACHLAM_004123"
     CDS             5426..5716
                     /locus_tag="ACHLAM_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7855574.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            5780..9796
                     /locus_tag="ACHLAM_004124"
     CDS             5780..9796
                     /locus_tag="ACHLAM_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO7855575.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAE
                     AGTDLSLLKRWLPRPQEMSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAQSGAYKAYLDGRPSGWYRDYRSADNSPTTWTFSRAENIDPLARLHLKASAQQNR
                     DEKERKLQQQYNKQAGYARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTIDADNMINVAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTH
                     GEVIIPTFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVSPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQETNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSTTTALRSYDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWIAFAEDLNADAPGNATVHSTDNAET
                     KTLSEPAGMHPVQQATPEPTEENKPQEEGVMSATDTGEESAAAAIDDDLNTVAEDLNA
                     DVTVMTTALHTDTAETASPSEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTENEA
                     ARPETPSLFSRLRRFLTGGKSRTAEPPVIQENTLTEAETATEAAAPPSPPETSPEDTS
                     SAEPDTIIFAPRRPNSPQRHNLDEIIKNLTYDELPDNTALCRVDGEPAFLDKLNRLEM
                     VDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPVQRA
                     ALEKLRQEKGIAHDAIVTHRPTPELNRLSPEETTMAQPAPPVSPKAETEQTAGSTAET
                     PHPEAPVTAKAEAPRAGTPEQPAASSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESV
                     TAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGNPVTLTLQAR
                     ETFRLSGETRDRIRNIWAMTPVSNGITVDNDHPHAGQHILEFPMKTFSQVMSQMQQHW
                     PQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNR
                     QLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVN
                     HDENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKA
                     EDRQEKTLQQAQSHSPGL"
     gene            9813..10163
                     /locus_tag="ACHLAM_004125"
     CDS             9813..10163
                     /locus_tag="ACHLAM_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7855576.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            10175..10870
                     /locus_tag="ACHLAM_004126"
     CDS             10175..10870
                     /locus_tag="ACHLAM_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO7855577.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            10881..>11007
                     /locus_tag="ACHLAM_004127"
     CDS             10881..>11007
                     /locus_tag="ACHLAM_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFO7855578.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPG"
BASE COUNT         2887 a         3165 c         2698 g         2257 t
ORIGIN      
        1 cagcagaaag ggcccaaata gaaaaagcaa aaatccttat aaaaatattc tttacaagcg
       61 ttcctatata caaacaaact atataaacca gccagaacag aatgactgca aaagaaacac
      121 acacggcccc cataaaaata aatgtcatcg aatcccatgg tttcaccagt acaaccggaa
      181 ccatggctgc cagaacaata agagcagcat tacccaacag cgtctctcta ataatatccc
      241 ttataatctc cagtttttct ttatgctgtt taattttcat acgaacctcg ttgatttcat
      301 cccgaaaagg aaaagattat ataacgaaat actttcaatc tcctgactcc gtctgctctg
      361 cagcataatg ccagaccagc ggcatgctaa atcacgtcct gtccgaatcc ttttcattca
      421 cgcttaaaac agagttaaaa gcactcttga aacacactcg atcataacga tcgaaataac
      481 tcgtttccgt tcagtaaaca cgctctttag acttaacata tgaaccgcac ggaggtgaaa
      541 tgaaacccaa cagcacaggc attgctgccc gcatgatgct ctccattgac agggcccgga
      601 ttagcgaagc tctgctcaat aaccgccagt tgcagtccac tcccctgcag atccgttatc
      661 cgcagggcgt cagagaagca ctcggaataa tgagcgagca actctccctt tctgtatccg
      721 acctgacgcg tatcctggtc gaagatgcat taactgaaat gtttctcccg gctgaaaaca
      781 tcgttcaccg tcttatctgc cggacagaac acatcatgca ggcacatgat gtctgcgtaa
      841 caacgatgac aacgttactg gctccctgga atatccgtcc tgccgttttc cgtgacccgg
      901 acaaactcgc agattatctg acaggagaga ttctgactgc tctggctgac tggttttatc
      961 tcaacccgga atggctgaac ggcagggtac gttatccttt aagccgtgcc ggtgagtggc
     1021 cagcaacaca ggatgcgttc tgccagctta tttcagccag cgataatgtg gatattatta
     1081 tctggcacag ttttcctttt gccggtgttc attcacagga atattgcggt gtactgttcc
     1141 gcaagaaaaa agaaattaat aaagctatca tttatcctgt actttcttta tcacctgtaa
     1201 gaatgaataa ggaaaaagaa aactggtttc acactgcccg caatttatat gcaaagacat
     1261 ccgtccggac cgtaacgctt cgtccggcac aggcagaata tctgatgaca ggaaaaatat
     1321 tacctgctga gttattcagt ataccgttat taccctggta ataacgcaga gaaaaaacca
     1381 ataaattttt aatggcacag tcgtgtcata ccgttgctgt acccaaaaca cctcaaacca
     1441 ggaaactgaa atgatgatga atatgcacat tacccgaact ctcatcgcat ctgctgtttt
     1501 attttccttt aattcaacag ccgcaacttc atattttgaa gcccgcaatg atgcaatggg
     1561 gggaaccggg gtagcctcct ctcactacgg tgtggcacct ctggcaaacc cggccctgct
     1621 gacaaaagcc ggtccggaag atgacttcag tcttctgctt ccgtccgttg gtgggcaatt
     1681 gtccgatccg ggtaatatca gtgacaacgc agacaaaatc agtgatgact ggaaagcctt
     1741 tgatcgtgcc atcgacagtc agagcaacgt gtcgcagact gctgcacgtc tgaagacgcg
     1801 acttcaggac ttccgtcaca cgcatgctga cgcacaggtg ggaacatctg ctgtggcgac
     1861 tcttcccggt gacaccctcg ctgccgctct gatgctgaaa agctacggta cggtcagtgt
     1921 cgacggtaaa gtcagtgatg ccgacctgaa ctatctggaa agcattgcaa acagtaacgg
     1981 tcaggatgtg gataaagaca gactgacctc tcaggctttt gcccgtgccg cactggtcac
     2041 cgatgtgggt attgctctgg ctactgaact ggaaacggcc ggacaaaagt ggtctctggg
     2101 cttcacacca aaattccagc gtgttgacct gtttaactac aacgtgctgg ttaaaaatta
     2161 cgacagcagc gctttcaaag gcgaccgtta ccacaacacc agaaacggca ttaacgctga
     2221 tatcggggcc agcatggacc tggatgacaa ctggacactg ggtctggtcg cacagaacct
     2281 gctcccccgc agtattgaga caaaggaagt gaacggtgtc actgaaactt tcaggatccg
     2341 cccgcaggtg actgccggtg tctcctggca caacgacatg ttcactacag cactggatgt
     2401 tgacctgacc ccggccagtg gcttcacatc agacagtaaa cgtcagtttg ctgccattgg
     2461 tgctgaattt aatgcctgga aatgggcaca gttacgtgca ggctaccgcc agaatctggc
     2521 cagcaatgat ggcagtgcgt tcaccgccgg gctgggtatc tcaccatttg atgtcgtcca
     2581 tattgatgtg gcgggtatag caggcacaga caacaactac ggtgccgttg cacaactgca
     2641 gttcacattc tgatcatctc tgccccggta ctgaccgggg tatccagacc tgcatcctcg
     2701 cgttttccga ccatcaaacg acacagccag acctgtagtg ctgcctctga tctggctgtt
     2761 atctgacata tcattttcgc atgaggaaat gcatgtttaa caaaaataaa acgcatatcg
     2821 aaaacaaagc ctttatggtg gaaccgttcc gggatccgac agctaacgaa tgtaatgtcg
     2881 tgatagcaaa cgatatttgt atccgggtag cactgtcacc ctctgaggtg ttccagccat
     2941 tcagcaaaaa cacgaaccag taaatcctcc ccacaacgct ttcttctttc actgctcagg
     3001 aaaaatacac catgaaccgt atgatacctg cgggatgtct cgccgtactc tgcctgaacg
     3061 gctgccatct ccccccggat aatacagctc cgaccagaaa gtatggtgct gcctctgatg
     3121 tcgccgtcgc ccggcatgcg cagtatcagc tatgggggca gggaatttac tctgcagccc
     3181 ttccggagcg tcagtccggc ggcagtctga aggctcacag cgaccgtgtt tcctttgact
     3241 gggaaggcga tgccctggaa ttgctcaacg aactggcccg tctgcgcggc cagcagttca
     3301 gctacagcgg cgtccgtctc cccttacccg tctcactgca cgtccgcgac atgacctttg
     3361 ctgatgtcct tcgtctcact gaagcccaga ctgcctggcg ggcaacactc tcgcagtcag
     3421 ccgggcgttt acatctgtct tttatgacgc cggagaaaaa atgaaaacac acctgtgtct
     3481 cctgctgtct gctctcctgc ttacaggctg tgccggacag catcaccgtt cttctgccgt
     3541 catgacagaa aacgcaccgc ccccggatat ccgtgcatgg ctgacgccct ccggaaacca
     3601 ggatgatggt atttcagata cccgtcgtca gatgctcacg gatgccggca gaacgctggg
     3661 attccgtgga ggaaaagcac agcgctcatg ggaactgatt caggcactga atgcccggga
     3721 aagtaccctg aatgccctgt atgatttccg gcctctcatc agcaaggaag gctggctgcc
     3781 tcctgttatc gatgaagcgc aggatgttgc acacatcaga ccggaccaga taagaacagc
     3841 ctcccgtgtc tggaccatcc tccgcccgga acgctttgtc agtaatccac ctggatggcg
     3901 tgactggctg ctgaaaggac tctcaaccac cgccacacca ggcactgaag gccgggttgt
     3961 gccggaagac cgggcacaac gcagactatg ggaaaacgcc ttacgtcagg gatggcagga
     4021 agggcgcctc aacgcagatc tcacactgga agctaatcag aagcgcctga cccgtgatta
     4081 ccggggcatg atgctttacg cccttctgtg gcgtcagggc atgatcacac gtccagatgt
     4141 cacagagcag atgcagacgg ttaccggcaa cgggcaaaaa ctcatcactg gcgatcgcgt
     4201 ccgccgcctg aaaacacatg ctgaatttac gctgcagaaa tcgcgctggc gccctcttgt
     4261 cagcaccgaa ggagagaaaa atgatatcac cagatgactt cggtaccttc cccttctccc
     4321 gttttacggc agaagagttt cgccattttt ttgcctggtg tgcccgccac cgcgtcagtg
     4381 atattgatct gaccggcggt tcaccggtat ccgtcagtcg ctttggacgg cgggttcgct
     4441 catccgtaaa tccccttccg tcctccatca tgatggcact gagtgatgag ctgtttgggc
     4501 gggaggttct tccccgggtt ctgtcaggtc atcccgtgga tcgtaccatt caggtcagcg
     4561 gagatgcctc cggtcgctat ggcctgcagc gaggtcagcg tgttcgcctg cgctgccacc
     4621 tgatacaggg aacatcggca acagaagaaa aagccatttc cgtcaccatg cgggtgatcc
     4681 cgtcagatat tccggacatc ctcactatga atattgaacc ggatctgctg gatgccatgc
     4741 tcagaaaaac cggcctgggc ttcatctgcg gagaaaccgg ctccgggaaa tccacactgg
     4801 cggcggcact gtatcgccac attctgaaaa atttccccga tcgcaaaata gtgacctatg
     4861 aagatccggt ggaatacatt ctgggcggag agaatgacct tctgcctccc catcaggccg
     4921 aaatcgggcg cgatgtggaa agttttgctg caggacttcg ctctgccgtt cgccgtaatc
     4981 cggacatcat tggtatcggt gaaatcaggg attatgaaac agcagatgcc gcagtccagg
     5041 cgggtaatac cggacacttt tgtatcggca ccatgcacac aaaatcaccc ggagaaacct
     5101 ttgcccgcct gctgggattg tttcctcctg aaatcaggga ttcgatggct gcagccacgc
     5161 tttccctcgt acagttcatt ctggtgcagg tactggtcag aacaaatgac ggcggcaggc
     5221 aggctgtccg ggaatacatc gtcatgactg acgaactgcg caatacgctc tccggacaat
     5281 cccatgctac atggggacat tacattgacg aaatcattcg tcaggaaaaa cggcggataa
     5341 gggatcaggt tctgaccatg tatcagaacg accgcattga gaaatcagaa gcagcgctct
     5401 tcatcccggc cggagagttc ccccgatgaa acgcgaaggc ttctggcggg atgtaacacc
     5461 accgctcaac atttatggtg tccccctgcc gtttaccatg atttacctgc tgttgtttcc
     5521 gtatccgtca aaaacgacat tctgggtgtg cagcggcatt ctgctgtttt ttatgctcct
     5581 gggatattcc ggctggacag tccgcaccct ctgtatacgt ctgtacagcc tgcttcaggg
     5641 taagcaggca tcgggacgtc cctggtggta ccgacaccgc acagaattac cccgtgaatg
     5701 gcacggacca gaatagccct cttttgtcca tcatcccggc gtatcacaac gccctcatat
     5761 ttaatccccg gagaacgaca tgaagtccag atatcgtacc tggcttgctg tcccgcctga
     5821 ggagacagaa gccgtaaaaa atgcagtccc cccgttaaat ggccgcaaag cggtagcatg
     5881 ggatccagag aaaaaactct ggtatgccga ggcgggtacc gacctttcac tgctgaagcg
     5941 ctggttaccc cgtccacagg aaatgtcgat ggatgccggc gatccggtca cagaatttgc
     6001 tcaggtcctg gaaaacgccg ggctggttat tcagggattg ccacagatgg atggcgccat
     6061 acacagagtc gccacccggg atgacaaaaa aggggcccaa agtggcgcat acaaggccta
     6121 tcttgatggc cgcccctccg gctggtaccg cgattaccgg agcgctgata acagtcccac
     6181 aacctggaca ttctcccgcg cagaaaatat cgatcctctc gccagactgc atctgaaggc
     6241 atccgctcag caaaatcgcg atgagaaaga acgtaaacta cagcaacagt acaacaaaca
     6301 ggccggctat gcccgctcct atcttaacgg gttgccccag gcaacagcac atgaatacct
     6361 gacacgcaag ggcatccggg ccgcgcctgg cgtgcggctg aacaataaaa atgagctggt
     6421 tattcccttc agcaacggcc ggggggaaat acgttcgtac cagcgtattc cggtcaccgg
     6481 tgggaaggat gcccgcattc tgaaaggctc tgagaaaagc ggcaactggt tcgccttcgg
     6541 aaccccggaa aatggccgtc ccctgttatt tgcggaaggt tacgctactg ccgcctccct
     6601 gcacgaagca accggccttc ctgttctgat gacaattgat gccgacaata tgattaatgt
     6661 ggcaaaaaat gcccgcaaaa tctggactga cagtcccttt gtcttctgtg ccgacaacga
     6721 ccatcaacgg aaaattaata aaggtgtttc aagtgcaaaa aaagctgccg caagcacgca
     6781 tggcgaagtc attattccga cctttacgga ggcggaaaaa gcacagggac tgaccgattt
     6841 caatgacctg gacgccagcc ggggacgaga cgcctttcag cgagccatga acgcacagtt
     6901 agaatatatc ggtatcccca cgcccaacaa tgacactact gaccgccgtg aagctatcgt
     6961 gatcggcaac ctgatattca caccggtaaa agatgagaag ccacaagtgt cccctgaaaa
     7021 cagacagagc acagcccctg aaccggcagt gtcggaacag aatcatcctt atgacatccc
     7081 tgcagaagag acagacaatc aggcatatga agatgactat gcagcatatg catccctgca
     7141 gtcggtaact gcagggacag cctccccgaa tgaaccgcag gtgaatgctg tttctgccat
     7201 tgacagcaac gcaaagaata cctctgccgc caccactgac gaccgaaatg tattagcaca
     7261 agaaaccaat gctgacgcca cggttacgac cacggctcac gacaccggca cagcagaaac
     7321 agattcacct tctgaacctg cagagattca gccagtgcag caggccagtc ctgaaccatc
     7381 ggctaaagat aaaccacagg agaacgtagt gtctgctact gataatgacg cagagaacac
     7441 ctcttccacc accactgccc tccggagtta cgacacaccg gaaacatccg tctccgacgc
     7501 aaccgggaca cagcacaccg ccacaacgca acctgaagaa ccacagcagg tctccatggg
     7561 cagcgctgac gactggattg catttgcgga agacctcaat gctgatgctc cggggaatgc
     7621 cactgttcac agcaccgaca acgcggagac aaagacactt tctgaacctg ccgggatgca
     7681 ccctgttcaa caggcaaccc ctgaaccaac ggaagaaaat aaacctcagg aagagggcgt
     7741 tatgtctgcc actgatacag gtgaagagag tgccgctgcc gctatcgatg acgacctgaa
     7801 cacagttgcg gaagacctca atgctgatgt tactgtgatg accacagctc tccacaccga
     7861 tacagcagaa acagcctcac cttctgaacc agcagaaatt cagcccgtac agcaggcaac
     7921 ccctgagcca acagcagaag ataaaccgaa ggaagagcgt cctgtgtctg ccactgagac
     7981 cagcacagag aatgaagcag cccgccctga aacaccatcg cttttcagcc gcctgcgccg
     8041 ttttctgacc ggtggaaaat cacgtaccgc cgaacctccc gttattcagg agaacacgct
     8101 tacggaggcc gaaactgcga cagaggccgc agcaccaccg tcaccacctg aaaccagccc
     8161 ggaagacaca tcgtcagcag aaccggacac gattatcttt gcgccccgcc ggcccaacag
     8221 tccgcagcgg cacaacctgg acgagattat taaaaatctt acatacgatg agttacccga
     8281 caatactgcg ctctgccgcg tcgacggcga accggcattc ctggataaac tcaaccgtct
     8341 ggaaatggtg gatggtgcca gtgctgatga caaaaaggtc ctggctgccc ttgctgtggc
     8401 caccaacttc tatggcggcg ttatcgagct gaccggcagt gatgccttta aacaaaaagc
     8461 catgcagctg attgtcgaat atgacatcaa ggttcgcatg aaactcccgg tccagagggc
     8521 tgcgctggaa aaactccgcc aggagaaagg gatagcgcat gatgccatcg tcacccacag
     8581 acccacgccg gaactcaacc gcctgtcacc ggaagagaca acaatggcac aaccggcccc
     8641 ccctgtgtca ccgaaagcag aaacagagca aactgcaggc agtacggctg aaacaccgca
     8701 tccggaagcg ccggtcacgg caaaagctga agccccccga gccggcacac cggaacaacc
     8761 cgccgcctcg tcgtctgcgc cagaaaaagc ccctgtcagt gcacagccgc agcaacccga
     8821 cacaccggag gaagagccgc ccggcaagct cagacccggt gaaagtgtga ctgccgtcct
     8881 gacaaactac ggagaaaaag agtatgcgcc cggaaaaggg cgctgctttt ttgtggagct
     8941 caccaaccgc agtggcaaac gggaatactg gggaaacggc ctggaagaac tggtcaaaaa
     9001 ccaccagaaa ggcaaccccg tcacactgac gctgcaggcg agagaaacat tccggctgtc
     9061 cggtgaaaca cgggatcgca tccgtaatat ctgggccatg actcctgtca gtaacggcat
     9121 taccgtcgat aatgaccatc ctcatgctgg tcagcatatc ctggaatttc cgatgaaaac
     9181 attcagtcag gtaatgtcac aaatgcaaca acactggcca cagataatgc aggaagttcg
     9241 ctcccccaaa aatcctccca ataatctgta cattggtgaa gacaggcaac ctgcatcagc
     9301 acctcaggat caatcaatgc gactccccct cgctggtaat gcccccaatg aactgatacc
     9361 agccatcgcc tctgccgata aggacaatcg tcagttgaat cttctgctgg tccacagcgc
     9421 agatgatcat ctgcagggcg tggtccgcct gaacggcacc ctttacccgg cactggccac
     9481 cccgtcagct gacaaccgtc agctggtcat caatgccctg acggataacg gtctgcagtt
     9541 tgccggttac ggtgaagctg ttaaccatga tgagaatact catcaacgtc cgtccccaca
     9601 aataatgcag tttcacctga agcaacagga ctccccgttg tttgccgcca tccacaaacc
     9661 ggaagagcaa ccggacaagc ttttccgctc tctgggtttt gagcagacct ggaaagagtg
     9721 gtctgacagc caaaaggcgg aggacaggca ggaaaaaact ctgcaacagg cacaatccca
     9781 cagccccggc ctgtgagccg gtaaggacga ccatgaacat gaaaaaaacg ctgctggtca
     9841 tactgaccag catctcgctg gcttctccgg ctgccgtggc agcaacctgt cgggcaggtg
     9901 ctgccgcaga agccggcagc aaagccgcct gggagcgggc gcgcacagcc agccaggcat
     9961 ggtcacagcg agaaaacgac gtcgcctcct ccctgcagtc ctgcctgtcc cgcattaagg
    10021 acgtcagcat cactcttcca caattcccca gcctggacaa aatccttcgc gatctggaaa
    10081 accaggtctg tgatgccgtc gtggatcggg tgaatgaaca cctgccgggg aatatcgacc
    10141 cctggaaaga ctataacctc tgaacaggag ccccatgagc gaagcacacc ccacatcccc
    10201 tgtctctccg gaaacagata acagcggaga tatttataaa caaacgctca gtaccattgc
    10261 acagtcagaa caacgggccg cactggttcc cggactggtc agggccttgc tgtgtagcag
    10321 cgcagctctc gtcatcagtc tgaccggcaa tatcatacag tactggcata acagcaacgt
    10381 aacgcgtgaa tactttgcca ccgacaatgg ccggctggtc cgcctggccc ccaccagtca
    10441 gccagcctgg agtcagagcg acgtcatgac gtttggcagc atgacgctga gcgaagcttt
    10501 taatctggat tttgtccact acaggaaaca aatcacgaca ctggcccccc gcttttccga
    10561 agccggtttt gccggctatg tgaatgctct tcaggcctcc aacattctgg acaccattaa
    10621 aaaggagaaa tttaacctca cggccaccat cggggccggc acgctggtga atcagggcca
    10681 gatggaaaac ggcatctggt tctggaaatt ccagtatccg gtcaggatgc gtctggtcgg
    10741 tcagaccagc acccgccccg agcaggcctt cacctttgaa atcactgtcc ggcgcgttga
    10801 cccacgaatc aaacccggcg gcatggaaat cagccagatg gtttcccgta acgccgcatc
    10861 aggaacctga gactcatatc atgcaaatac atcatcttct tggcgcactg cttattgtgt
    10921 gcggcactgt cacggctgca gataacccgg gctggcagaa cgcacgaacc ccacaggctg
    10981 gcggaaagac aggacaggct cccggga
//
LOCUS       DAZGAP010000173        10934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGAP010000173 DAZGAP010000000
VERSION     DAZGAP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.174"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..1234
                     /gene="yghO"
                     /locus_tag="ACHLAM_004128"
     CDS             92..1234
                     /gene="yghO"
                     /locus_tag="ACHLAM_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO7855579.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHYGMIDAIDD
                     PQVFAALFGAAEAWLKSQGANKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1241..2005)
                     /gene="glcC"
                     /locus_tag="ACHLAM_004129"
     CDS             complement(1241..2005)
                     /gene="glcC"
                     /locus_tag="ACHLAM_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO7855580.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2256..3755
                     /gene="glcD"
                     /locus_tag="ACHLAM_004130"
     CDS             2256..3755
                     /gene="glcD"
                     /locus_tag="ACHLAM_004130"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO7855581.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3755..4807
                     /gene="glcE"
                     /locus_tag="ACHLAM_004131"
     CDS             3755..4807
                     /gene="glcE"
                     /locus_tag="ACHLAM_004131"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO7855582.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4818..6041
                     /gene="glcF"
                     /locus_tag="ACHLAM_004132"
     CDS             4818..6041
                     /gene="glcF"
                     /locus_tag="ACHLAM_004132"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO7855583.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6046..6450
                     /locus_tag="ACHLAM_004133"
     CDS             6046..6450
                     /locus_tag="ACHLAM_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO7855584.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6472..8643
                     /gene="glcB"
                     /locus_tag="ACHLAM_004134"
     CDS             6472..8643
                     /gene="glcB"
                     /locus_tag="ACHLAM_004134"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO7855585.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPSCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPHEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            8999..10681
                     /gene="glcA"
                     /locus_tag="ACHLAM_004135"
     CDS             8999..10681
                     /gene="glcA"
                     /locus_tag="ACHLAM_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO7855586.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            complement(10709..>10934)
                     /locus_tag="ACHLAM_004136"
     CDS             complement(10709..>10934)
                     /locus_tag="ACHLAM_004136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855587.1"
                     /translation="IVLTIAIAKATDSTVFAHQANIISTNLFCVIKNRYSHVLNYLAG
                     YLNAKHYCCMDNKEGNNFQLIRQNNVNVAG"
BASE COUNT         2543 a         2733 c         3068 g         2590 t
ORIGIN      
        1 ttgtttgtat tcattgggtt gattattaag atgccgttcg ttgctgttgc aacctgtttt
       61 tcaccgttaa ttatttgtta tccacagaga tatggaatgc gatctgctga tgattaaaat
      121 tgaaaaagta attaataaaa atgaccttaa ggcatttatc gcttttccgt catcacttta
      181 tcccgatgat ccaaactgga tacccccttt attcattgaa cgcagcgagc atttgtctgc
      241 gaagaatccg gggacggacc atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca
      301 gatagttggg cgtattaccg cgcaaatcga taccttgcac cgtgagcgtt acggtgaaga
      361 taccggtcat tacggaatga ttgatgccat tgatgatccg caggtttttg ccgcgttgtt
      421 tggcgcagcg gaagcgtggt taaagtcaca aggtgcaaat aagatcagcg ggcctttcag
      481 cctgaatatc aatcaggaaa gcggattact gattgaaggt tttgacactc cgccctgtgc
      541 gatgatgcca cacggcaaac cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa
      601 aggcattgat ttactggcgt ggtggatgca gcgaaccgat ctcacgttct ctccggcgct
      661 aaaaaaactg atggatcagg tgcgcaaaaa agtgaccatt cgctgcatca atcgccagcg
      721 gtttgccgaa gagatgcaga tcctgcgtga gattttcaat tctggctggc agcacaactg
      781 gggatttgtg ccgtttaccg aacatgaatt cgcgaccatg ggcgatcaac ttaaatatct
      841 ggtgccggac gatatgatct acatcgctga gattgattct gcaccctgcg cgtttattgt
      901 tggcttgccg aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg
      961 ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc gagtgccgct
     1021 gatgggcgtg cgggacgagt atcagttcag ccgcatcggc ccggtaattg cgctgttatt
     1081 gattgaagcc ttacgcgatc cgtttgcccg tcggaagatc gatgcgctgg agatgtcatg
     1141 gatcctcgaa accaatacag gcatgaataa catgctggaa cgcattgggg cggagccgta
     1201 caagcgttac cgattgtatg aaaagcagat ttgatggcag tcacccctgc ttcatctcca
     1261 gcggcaccgc cgagcgaatc aaatggtggc cttccagctc gatatcgtgg agattctttt
     1321 tcacggtccg cacatgatca cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca
     1381 ccgcgttgta gatccgcgca tgctggcgat cgatctgctt tttttgctgt ggtcgatggt
     1441 agagattatt tacgctggca aacactgaat taaacatcag atcggtcaat gattgcagcg
     1501 taaacaccag cacctgattg tgagaagcct gacaaatggc gagatggaaa gcgtgatcca
     1561 actgcgcatg ttcgatcagc gaaatctctt tgttgttctc actggcggcg agcatttttt
     1621 cataacagcg ggttatcaca acaaaatcag cctgggttcc cagcgttgcc gccagccttg
     1681 ccgattcgcc ctccagtaat gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg
     1741 tactgaacag atggatcagc gggctggcgt cctgcacccg attaagccgt gcgacacgag
     1801 aatcgcgacc ctgcgccgtt tcaataatcc cgcgcccgcg cagcacggtc agcccttcac
     1861 gcagtgcgga gcgtgagaag ccgagctttt cacacagtcg acgttccgag ggaagcggct
     1921 gaccgacctt caacacgccg tcgataatta accgttcgat actctctgca accacttcgc
     1981 aaatagggcg acgttcatct ttcattcccg gacctcgtgc acaggtagga ccaatttttc
     2041 tgttcttaac tcgcaaaaca cgcacatcac gcaagtgatt atgttacatc aatttaacat
     2101 taagttaacc aaggcaaggt cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag
     2161 agatagacga aaacgaaaag cccgcttaat aactgttcac agaagcagcg cgcaaaaatc
     2221 agctgccaca caacacaaca aagcgaagcc tattcatgag catcttgtac gaagagcgtc
     2281 ttgatggcgc tttacccgat gtcgaccgca catcggtact gatggcactg cgtgagcatg
     2341 tccctggact tgagatcctg cataccgatg aggagatcat tccttacgag tgtgacgggt
     2401 tgagcgcgta tcgcacgcgt ccattactgg ttgttttgcc aaagcagatg gaacaggtga
     2461 cggcgattct ggctgtctgc caccgcctgc gtgtaccggt ggtgacccgt ggtgcaggca
     2521 ccgggctttc tggtggcgcg ctgccactgg aaaaaggtgt gttgttggtg atggcgcgct
     2581 ttaaagagat cctcgacatt aaccccgttg gtcgccgcgc gcgcgtgcag ccgggcgtgc
     2641 gtaatctggc gatctcccag gccgttgcgc cgcataatct ctattacgcg ccggatcctt
     2701 cctcacaaat cgcctgttca attggcggca atgtggcgga aaatgccggt ggcgttcact
     2761 gcctgaaata tggtctgacc gtacataacc tgctgaaaat tgaagtgcaa acgctggacg
     2821 gcgaggcgct gacgctggga tcggatgcgc tggattcgcc tggttttgac ctgctggcgc
     2881 tgttcaccgg atcggaaggt atgctcggcg tgaccaccga agtgacggta aaactgctgc
     2941 cgaagccgcc cgtggcgcgg gttctgttag ccagctttga ctcggtagaa aaagccggac
     3001 ttgcggttgg tgacatcatc gccaatggca ttatccccgg cgggctggag atgatggata
     3061 acctgtcgat ccgcgcagcg gaagatttta ttcatgccgg ttatcccgtc gacgccgaag
     3121 cgattttgtt atgcgagctg gacggcgtgg agtctgacgt acaggaagac tgcgagcggg
     3181 ttaacgacat cttgttgaaa gcgggcgcga ctgacgtccg tctggcacag gacgaagcag
     3241 agcgcgtacg tttctgggcc ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc
     3301 cggattacta ctgcatggat ggcaccatcc cgcgccgcgc cctgcctggc gtactggaag
     3361 gcattgcccg tttatcgcag caatatgatt tacgcgttgc caacgtcttt catgccggag
     3421 acggcaacat gcacccgtta atccttttcg atgccaacga acccggtgaa tttgcccgcg
     3481 cggaagagct gggcgggaag atcctcgaac tctgcgttga agttggcggc agcatcagtg
     3541 gggaacatgg catcgggcgc gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg
     3601 aaatcacgac cttccatgcg gtcaaggcgg cgtttgatcc cgatggtttg ctgaaccctg
     3661 ggaaaaacat tcccacgcta caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg
     3721 gtcatttacc tttccctgaa ctggagcgtt tctgatgcta cgcgagtgtg attacagtca
     3781 ggcgctgctg gagcaggtga atcaggcgat tagcgataaa acgccgctgg tgattcaggg
     3841 aagcaatagc aaagcctttt taggccgccc tgtcaccggg caaacgctgg atgtgcgttg
     3901 tcatcgaggc attgttaatt acgacccgac cgagctggtg ataaccgcgc gtgccggaac
     3961 gccgctggta gccattgaag cggcgctgga gagcgccggg caaatgctcc cctgcgagcc
     4021 gccgcattat ggtgaagaag ccacctgggg cgggatggtc gcctgcgggc tggcggggcc
     4081 gcgtcgcccg tggagcggtt cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg
     4141 cactggaaaa catctgcgtt ttggtggcga agtgatgaaa aacgttgccg gatacgatct
     4201 ctcacggtta atggccggaa gctacggttg tctgggtgtg ctcactgaaa tctcgatgaa
     4261 agtgttaccg cgaccacgcg ccaccctgag tctgcgtcgg gaaatcagcc tgcaagaagc
     4321 catgaatgaa atcgcccagt ggcaacttca gccattaccc attagtggct tatgttactt
     4381 cgacaatgcg ttgtggatcc gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga
     4441 actgctgggg ggcgaagagg ttgcgggtca gttctggcag caattgcgtg aacaacaact
     4501 gccgttcttc tcgttaccag gtaccttatg gcgcatttca ttacccagcg atgcgccgat
     4561 gatggattta cccggcgagc aactgatcga ctggggcggg gcgttacgct ggctgaaatc
     4621 gacagccgat gacaatcaaa tccatcgcat cgcccgcaac gctggcggtc atgcgacccg
     4681 ctttagtgcc ggagatggtg gctttgcccc gctaccggct cctttattcc gctatcacca
     4741 gcagcttaaa cagcagctcg acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga
     4801 actttgagga gcaggctatg caaacccgat taacagaaga gatgcggcag aacgcgcgcg
     4861 cgctggaagc cgacagcatc ctgcgcgcct gtgttcactg cggattttgt accgcaacct
     4921 gcccaaccta tcagcttctg ggcgatgaac tggacgggcc gcgcgggcgc atctatctga
     4981 ttaaacaggt actggaaggc aacgaagtca cgcttaaaac acaggagcat ctcgatcgct
     5041 gtctcacttg ccgcaattgt gaaaccacct gtccttctgg tgtgcgctat cacaatttgc
     5101 tggatatcgg gcgtgatatt gtcgagcaga aagtgaaacg cccactgccg gagcgaatgc
     5161 tgcgcgaagg attgcgccag gtagtgccgc gtccggcggt cttccgtgcg ctgacgcagg
     5221 tagggctggt gctgcgaccg tttttaccgg aacaggtcag agcaaaactg cctgctgaaa
     5281 cggtgaaagc taaaccgcgt ccgccgctgc gccataagcg tcgggtttta atgctggaag
     5341 gctgcgccca gcctacgctt tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc
     5401 gtctggggat cagcgtgacg ccagctaacg aagcaggctg ttgtggcgcg gtggactatc
     5461 atcttaatgc gcaagagaaa gggctggcgc gggcgcgcaa taatattgat gcctggtggc
     5521 ccgcgattga agcaggtgtc gaggcaattt tgcaaaccgc cagcggttgc ggcgcgttcg
     5581 ttaaagagta tgggcagatg ctgaaaaacg atgcgttgta tgccgataaa gcgcgtctgg
     5641 tcagtgaact ggcggtcgat ttagtcgaac ttctgcgcga ggaaccgctg gaaaaactgg
     5701 caattcgcgg cgataaaaaa ctggccttcc actgcccgtg taccctacaa catgcgcaaa
     5761 agctgaacgg cgaagtggaa aaagtgttac ttcgtcttgg atttacctta acggacgttc
     5821 ccgacagcca tctgtgctgc ggttcagcgg gaacatatgc gttaacgcat cccgatctgg
     5881 cacgccagct gcgggataac aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg
     5941 tcaccgccaa cattggttgc cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgtc
     6001 actggattga aattgtagaa caagcccttg aaaaggaata acaaaatgaa aactaaagtc
     6061 attcttagcc agcaaatggc gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa
     6121 aataactggt ctgtttccat tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt
     6181 cgcatggacg attgcgcgcc gattgcggct tacatctccc aggagaaagc gcgtaccgcc
     6241 gcgctggggc gtcgtgaaac taagggctat gaagagatgg tgaacaacgg acgtaccgcg
     6301 ttcgtgactg cgccgttact gacgtcgctg gaaggtggcg taccggttgt tgtggatggg
     6361 caaattattg gtgccgtggg cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg
     6421 aaagcggcag cagcggtgtt ggcgaaataa gcgaaaacga ggagataaac aatgagtcaa
     6481 accataaccc agggccgttt acgcattaac gccaatttta aacgttttgt ggatgaagaa
     6541 gttttaccgg gaacagggtt ggacactgcg gcgttctggc gcaattttga tgagatcgtt
     6601 cacgatctgg caccagaaaa tcgtcagttg ctggcagaac gcgatcgcat tcaggcggcg
     6661 cttgatgagt ggcatcgcag caatccgggg ccggtaaaag ataaagcggc ctataaatct
     6721 ttcctgcgtg aactgggcta cctggtgccg caaccggagc gcgtgacggt ggaaaccacg
     6781 ggcattgaca gcgaaatcac cagccaggcg gggccacagc tggtggttcc ggcaatgaac
     6841 gcccgctacg cgctgaacgc ggcgaacgct cgctggggct cactgtacga tgcgttatac
     6901 ggcagcgaca tcatcccgca ggaaggggcg atggtcagcg gctacgatcc gcaacgcggt
     6961 gagcaggtta tcgcctgggt tcggcgtttc ctcgatgaat cactaccgct ggaaaacggc
     7021 agctatcagg atgtggtggc gtttaaggtg gtcgataaac aattacgcat ccagttgaaa
     7081 aatggtaaag aaaccacgtt acgtaccccg gcgcagtttg tcggttaccg tggcgatgcc
     7141 gctgcgccgt cctgcatttt gctgaaaaat aacggcctgc atattgagct acaaatcgat
     7201 gccaacgggc ggattggcaa agacgattcg gcgcacatca acgatgttat cgtcgaagcg
     7261 gccatcagta ccattctcga ctgcgaagat tcggttgcgg cggttgatgc ggaagataaa
     7321 atcctgctgt accgcaatct gctgggtctg atgcagggga ctctgcaaga gaaaatggag
     7381 aaaaacggtc ggcaaatcgt gcgtaaactg aatgacgatc gtcagtacac cgctgccgac
     7441 ggctctgaaa tttctctgca cgggcgttcg ctgctgttta tccgcaacgt gggtcatttg
     7501 atgaccattc ctgtgatttg ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat
     7561 ggcgtcatga ctggcgcgat tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact
     7621 ggcagcgtct atattgtgaa accgaaaatg cacggtccgc atgaagtggc gttcgccaac
     7681 aaactgttta cccgcattga gacaatgctc ggtatggcac cgaataccct gaaaatgggc
     7741 attatggatg aagaacgtcg gacctcgctg aacttgcgta gctgtatcgc tcaggcgcgt
     7801 aaccgcgtgg cgttcatcaa taccggtttc ctcgaccgta ccggcgatga aatgcattcg
     7861 gtgatggaag ctggcccgat gctgcgtaaa aaccagatga aatcgacgcc gtggatcaaa
     7921 gcctacgaac gtaataacgt gctttccggt ctgttctgtg ggctgcgcgg taaagcgcaa
     7981 atcggtaaag gcatgtgggc aatgccggac ctgatggcag acatgtacag ccagaagggc
     8041 gaccagctgc gtgccggggc aaacacggcc tgggttccgt cgccaactgc cgctacgctc
     8101 catgcgctgc actaccacca aaccaacgta cagagcgtgc aagccaacat tgcccagacc
     8161 gagttcaatg ctgaatttga accgctgctg gacgatctgc tgactattcc ggttgctgaa
     8221 aacgctaatt ggtcggcgca agagatccaa caagagctgg ataacaacgt gcaggggatt
     8281 ctggggtacg tggtgcgttg ggtggagcag gggattggtt gttcaaaagt gccggatatt
     8341 cacaacgtgg cgttgatgga agaccgcgca acgctgcgta tttccagcca gcatatcgcc
     8401 aactggttac gtcacggtat tctgaccaaa gatcaggtgc aggcgtcgct ggagaatatg
     8461 gcgaaagtgg ttgatcagca aaacgctggc gatccggctt atcgtccaat ggtggagaat
     8521 ttcgctaact cgtgtgcttt taaagctgcc tgcgatttga tcttcctcgg agtgaaacag
     8581 ccaaacggct ataccgaacc gttattacac gcatggcgtt tacgcgaaaa agaaaatcat
     8641 taatagcgtc aaaaaaagcc ccggcaagat ttattgtcgg ggctatgata gtaactgcgg
     8701 tagtacattt atcccggaaa taaccggagc gattatcagg ggttaacaat attaaatacc
     8761 cgtagcgcat cactagcggg attaaaacaa caatatggac atcttaacga tactctgcgc
     8821 ctgcgtgtgc ggaacagaaa cccttacccg atatacttgt tatgccagag gtagcaattg
     8881 cgttagcggc gtactgttgc tgtaaagtat aacgcctata ttttacagtt cagtcctctt
     8941 ttgcttatta caaccttaag tatatcaagc atataaagat aataagagac tgaacaatat
     9001 ggttacctgg acccaaatgt atatgccgat gggaggactg gggctatccg ctctggtcgc
     9061 cctgatccca ataatattct tcttcgttgc actcgcggta ttacgtctga aaggacatgt
     9121 cgctggagca ataaccctta tattatctat tctgattgcg atattcgcct ttaaaatgcc
     9181 gattgatatg gcatttgctg ctgcgggcta tggctttatt tatggtttat ggcctattgc
     9241 gtggattatt gtcgcggcgg tattcctgta taaattaacc gttgccagcg gccagttcga
     9301 tattatccgc agctcggtta tctccatcac cgacgatcag cgtttgcagg tgttactgat
     9361 tggtttctcc tttggtgcgc tgctggaagg ggcggctggc tttggcgcac cggtggcgat
     9421 taccggtgct ctactggtgg gcctgggctt caaaccgcta tacgcggcgg ggctgtgtct
     9481 gattgccaac actgcgccgg tggcgtttgg tgcgttgggt gtgccaattc tggtcgccgg
     9541 tcaggtaacg ggaatcgatc cgttcgacat tggcgcaatg gcgggacgtc agttaccgtt
     9601 cctgtcggtt cttgtgccgt tctggctggt agcaatgatg gacggctgga aaggggtgaa
     9661 agagacgtgg ccagcggcgc tggttgcagg gggaagcttc gctgtcactc agttctttac
     9721 ctctaactat attggtccgg aactgccgga tattacttcg gcgctggtga gtatcgtctc
     9781 actttcttta tttcttaaag tctggcggcc aaaaaatacc gaaacggcaa tcagtatggg
     9841 acaatccgca ggtgcgatgg tggtgaataa accgtcttct ggcggtcccg tgccttcaga
     9901 atatcgtctg gggcaaatca ttcgtgcgtg gtcaccgttt ttaatcttaa cggtgttggt
     9961 caccatctgg accatgaagc cgtttaaagc gttatttgct ccgggcggcg cgttttattc
    10021 gctggtgatt aatttccaga tccctcattt gcatcaacaa gtgttgaaag cggcccccat
    10081 tgtcgcccag ccgacgccaa tggacgcggt gtttaaattc gaccccctct ctgctggtgg
    10141 caccgctatt tttattgcgg cgattatctc gatcttcatc ctcggcgtgg ggatcaagaa
    10201 aggtattggt gtctttgccg aaacgctaat tagtctgaag tggccgatac tgtcgattgg
    10261 catggtgctg gcatttgcct tcgtgaccaa ctattccggc atgtccacca cgctggcgct
    10321 ggtactggct ggtacaggcg taatgttccc gtttttctca ccgtttctcg gctggctggg
    10381 cgtattcctt accggctcgg acacctcctc taacgccctg tttggttcac tgcaatcgac
    10441 cacggcgcag caaatcaacg tctctgacac cctgctggtg gcagcaaata ccagcggcgg
    10501 cgtaactggc aagatgatct ccccgcaatc tatcgccgtg gcctgcgccg cgacgggcat
    10561 ggtgggccga gaatctgaac tgttccgcta caccgtgaag cacagtctga tttttgccag
    10621 cgttatcggc attatcacgc tgttgcaggc gtatgtgttt accgggatgt tagtttcgta
    10681 agtcaataat gccggatgat cgttaatatc atccggctac atttacatta ttctgtctta
    10741 ttaattgaaa attatttccc tctttattat ccatgcagca ataatgtttt gcgttcagat
    10801 aacctgctaa ataattaaga acgtgagaat atctattttt tatcacgcaa aacaagtttg
    10861 tactaattat gttcgcttga tgagcaaaca ccgtgctatc tgttgctttt gcaatcgcaa
    10921 tcgttaaaac tatc
//
LOCUS       DAZGAP010000174        10768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGAP010000174 DAZGAP010000000
VERSION     DAZGAP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.175"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..1322
                     /gene="pepT"
                     /locus_tag="ACHLAM_004137"
     CDS             93..1322
                     /gene="pepT"
                     /locus_tag="ACHLAM_004137"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO7855588.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(1468..2589)
                     /gene="roxA"
                     /locus_tag="ACHLAM_004138"
                     /gene_synonym="ycfD"
     CDS             complement(1468..2589)
                     /gene="roxA"
                     /locus_tag="ACHLAM_004138"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO7855589.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETSWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(2665..4125)
                     /gene="phoQ"
                     /locus_tag="ACHLAM_004139"
     CDS             complement(2665..4125)
                     /gene="phoQ"
                     /locus_tag="ACHLAM_004139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO7855590.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(4125..4796)
                     /gene="phoP"
                     /locus_tag="ACHLAM_004140"
     CDS             complement(4125..4796)
                     /gene="phoP"
                     /locus_tag="ACHLAM_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO7855591.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(4965..6335)
                     /gene="purB"
                     /locus_tag="ACHLAM_004141"
     CDS             complement(4965..6335)
                     /gene="purB"
                     /locus_tag="ACHLAM_004141"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO7855592.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(6339..6980)
                     /gene="hflD"
                     /locus_tag="ACHLAM_004142"
     CDS             complement(6339..6980)
                     /gene="hflD"
                     /locus_tag="ACHLAM_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO7855593.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(7016..8122)
                     /gene="mnmA"
                     /locus_tag="ACHLAM_004143"
                     /gene_synonym="trmU"
     CDS             complement(7016..8122)
                     /gene="mnmA"
                     /locus_tag="ACHLAM_004143"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO7855594.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            complement(8176..8637)
                     /gene="nudJ"
                     /locus_tag="ACHLAM_004144"
     CDS             complement(8176..8637)
                     /gene="nudJ"
                     /locus_tag="ACHLAM_004144"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO7855595.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(8647..9300)
                     /gene="rluE"
                     /locus_tag="ACHLAM_004145"
     CDS             complement(8647..9300)
                     /gene="rluE"
                     /locus_tag="ACHLAM_004145"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO7855596.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            9472..10722
                     /gene="icd"
                     /locus_tag="ACHLAM_004146"
     CDS             9472..10722
                     /gene="icd"
                     /locus_tag="ACHLAM_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO7855597.1"
                     /translation="MESKVVVPAQGKKITLQNSKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         2643 a         2763 c         2759 g         2603 t
ORIGIN      
        1 tattcccgct tgcagggcta aaagtgacct gacgcaatat ttgtcttttc ttgcttctta
       61 ataatgttgt cacaaaaagt gagggtgact acatggataa actacttgag cgatttttga
      121 actacgtgtc tctggatacc caatcaaagg caggggtgag acaggttccc agcacggaag
      181 gccaatggaa gttattgcat ctgctgaaag agcagctcga agagatgggg cttatcaatg
      241 tgaccttaag tgagaagggc actttgatgg cgacgttacc ggctaacgtc cctggcgata
      301 tcccggcgat tggctttatt tctcatgtgg atacctcacc ggattgcagc ggcaaaaatg
      361 tgaatccgca aattgttgaa aactatcgcg gtggcgatat tgcgctgggt atcggcgatg
      421 aagttttatc accggttatg ttcccggtgc tgcatcagct actgggtcag acgctgatta
      481 ccaccgatgg taaaaccttg ttaggtgccg atgacaaagc aggtattgca gaaatcatga
      541 ccgcgctggc ggtattgcaa caaaaaaaca ttccgcatgg tgatattcgc gtcgccttta
      601 ccccggatga agaagtgggc aaaggggcga agcattttga tgttgacgcc ttcgatgccc
      661 gctgggctta caccgttgat ggtggtggcg taggcgaact ggagtttgaa aacttcaacg
      721 ccgcgtcggt caatatcaaa attgtcggta acaatgttca cccgggcacg gcgaaaggag
      781 taatggtaaa tgcgctgtcg ctggcggcac gtattcatgc ggaagttccg gcggatgaaa
      841 gcccggaaat gacagaaggc tatgaaggtt tctatcacct ggcgagcatg aaaggcaccg
      901 ttgaacgggc cgatatgcac tacatcatcc gtgatttcga ccgtaaacag tttgaagcgc
      961 gtaaacgtaa aatgatggag atcgccaaaa aagtgggcaa agggttacat cctgattgct
     1021 acattgaact ggtgattgaa gacagttact acaatatgcg cgagaaagtg gttgagcatc
     1081 cgcatattct cgatatcgcc cagcaggcga tgcgtgactg cgatattgaa ccggaactga
     1141 aaccgatccg cggcggtacc gacggcgcgc agttgtcgtt tatgggatta ccgtgcccga
     1201 acctgttcac tggcggttac aactatcatg gtaagcatga gtttgtgact ctggaaggta
     1261 tggaaaaagc ggtgcaggtg atcgtccgta ttgccgagtt gacggcgcaa cggaagtcgt
     1321 gattgcccga tgcgtatgtg gactgccaat taccgggatg aaacctttat attgacactg
     1381 gtccacatac ttatcgtgcc tacaggcgtt cagtgcatgt aggcacgata aggcgcaatc
     1441 agcgccgcat ccggcttaaa tccaaactta cccttcgaag aaccagtatc cgctattgac
     1501 cagcgccgcg agcatcgcga ggaatgacgg atcttccagc gcatcgccaa aattctccgc
     1561 ggtcagcgca atgttgctgg cgagcgcatc cagtgccgga cggtgcgggg aatcgatctt
     1621 ctcaccattg gcatacacgt cgtcgccaat gcgcaatacg cgcagacccc ccaggcgcac
     1681 cagcacatca ccttgtttca gcgcatcgta gatttcatcc ggctgataag gcggctctgg
     1741 cggcgcgata tccagttcat gacgtgactg ggatataaac tcgccaaacc attgcttaaa
     1801 atgttccggc tggttgatca attcgagcat catctcacgc agtttatcca tctcttgcgg
     1861 cagaacgtcc gcaggatgag cgcgaggtgg aacatccgga tcgctgtagt agttgccgcc
     1921 cagttcacgt tgcagcacat aatcggcaaa tccactaatc agttcccgcg tatttggcgc
     1981 gcgaaaaccc acggaatagt tcatcgcatt ttccagcgcg tagccttcat gcgggaatcc
     2041 tggcggaata taaagaatat caccaggctc cagctcttca tcgatgatgg cttcgaacgg
     2101 atcgacctgt aacagatccg gatgtgggca gtgctgtttc atttgcagct tttcgcccac
     2161 tcgccagcga cgacgtccgg taccctgaat gataaacacg tcgtactgat cgagatgcgg
     2221 gccgacgccg ccgccaggta cagaaaaaga gatcatcagg tcatcaatac gccagtccgg
     2281 cagttcacgg aacggtcgca tcagcgcggc ggtcggctca tgccagtggt tcactgcttg
     2341 cactaacaat gaccagctag tttcaccgag atgatcgtag ctttcgaatg gaccgtggct
     2401 gacctgccat ttgccatcct gatgactgac cagtcgactg tcgacttcgc tttccatcgc
     2461 cagacccgcc aactcgtctg gagagatcgg gtcaataaaa ttattaaagc cgcgttttaa
     2521 caccaccggg cgtttctgcc agtgacgttc aagaaaatcg ggccagttaa gagtgagttg
     2581 gtattccatg ttaagcatcc acaggctggt atctgcaacc gattataacg gatgcttaac
     2641 gtaatgcgtg aagtatggac atatttattc atctttcggc gcagaatgct ggcgaccaaa
     2701 aatcacctcc atccgcgcac cgcccagcat gctctctccg gcgacgattt taccctcata
     2761 ttgctcggtg atttcgcggg ctaccgccag ccccacacct tgcccagggc gtaaagtatc
     2821 aacccgttga ccacggtcga aaatgacctc tcgcttgctt aatggaatac cggggccatc
     2881 atcctcgacc acaatataga gatgctcgtc ggtttgcctt gcagaaattt cgacaaactc
     2941 gaggcaatat ttacaggcat tatccagcac attgcccatc acctcgacaa aatcgttctg
     3001 ctcaccgaca aagctgatct ctggcgaaat atcgagagag atattgaccc ctttgcgttg
     3061 atacactttg ttcagcgccg aggtgagatt gtccagcagt ggagcgaccg gatgcagctc
     3121 gcggctgagc aatgtcccgc cgcgcatact ggcacgatgc aggtagtagc caatttgctg
     3181 tgaaatgcgg ctgatttgct ccagcattac cggttcagca tcactgacgc tcatcttttc
     3241 actacgcaga gaacgcagcg tactttgcag caccgccagt ggcgttttca gactatgggt
     3301 caggtcggtg agcgtcgtac gatatttgtc gtaacgttcg cgttcacttt ttaacaatcg
     3361 gttcaggttt cgtaccagac tggtcagttc tcgcgttgtg gctggattga gcaattcgcg
     3421 gttatgttct tccagttcgc ggacttcttt tgccagggct tcgatggggc gtaaactcca
     3481 ccaggcggcg acccacagca gcgggatcac taacagcaga ttggctgaga gcacatagat
     3541 aaaccagctc cagaccatat aggaactttt tagctccacc ggaatggtat ccaccaccac
     3601 aatggtcaac tttggcatcc gcgatgttgc cgggtagacg tttaccgcca ccgagtgggt
     3661 catctccgcg tcgtcatcat cttcccgcac ttcctgcaac tgttgctgta tcgaatgatc
     3721 tccactcagc aagaggctgg tatcgttaac atccgcttca atttcatgaa aaccattcga
     3781 tttcagccag tcaggctgga tcatcttcat cagccagggc acgtcacgtt gcgcccataa
     3841 aagctgcccg ttctcatcat aaattagtgt catcgtgggg ctttgcttgt cgatattttc
     3901 gggtaactcg acatgcaact tattgttttc ccacttcgca agggtataga acagattgct
     3961 ctcgccacgt aacagccgaa acgtggtttt atcgaaactg acgctataac cgatcagcgc
     4021 gaccattccg taggcaagcg aaagcaccag tactaccgct gccgttgcca acagaaaacg
     4081 tacccgcagc gagagcggga aaaaaagacg cagtaatttt ttcatcagcg caattcgaac
     4141 agatagccct ggccgcgaac ggtggtaatc acttcttgcg gatattgtgc ctgaattttt
     4201 ttgcgcagac gtcccatcag tacatcaatg gtatggcttt cccgcagctc cgcatccgga
     4261 tagagttgga gcattaacga atctttgctc accactttgc cattattgcg tatcaacgtt
     4321 tccataatgg tgtattcgaa cgcggtcagt ttgatcactt cgtcattaat agataattca
     4381 cggcgagaga gatcaacctg aaacggtggt agcgaaatga cctgtgaagc cagaccgcta
     4441 ttacgccgca ttaatgcctg cattcgcgcc atcacctctt caatatgaaa cggtttagtc
     4501 acgtaatcat cagcgccagc acttaatact tcgactttgt cctgccagct ttcacgggcg
     4561 gttaatacca gaatcggcag tgaaacatca ttgctacgcc agcggcgaat cagtgacaga
     4621 ccgtcctcgt caggcaaacc gagatcgaca atcgcaatat ccggtaaatg ttcattgaga
     4681 taataatcgg cttctttggc atcttctgcg tcatcgacct gatgaccagc atcctgaatc
     4741 tgaactttaa gatggtgacg taacaacgca ttgtcttcaa caaccagtac gcgcattttt
     4801 atttctccct gtcttaatta ttaaaatagt gtaacgcgat tatgtggtta tgggggtaaa
     4861 cattaaataa accagcggga aggggaggta aagtgaaaaa acaaaaagtg gataatctta
     4921 ataagcaggc cggacagcat cgccatccgg cactgatttg aggcttattt cagctcatca
     4981 accatggtga tggcgcgacc aatatagtta gccggcgtca tcgctttcag gcgggctttc
     5041 tcttcttctg gcaacgccag accgtcgata aactgcttca tgccttcggc gtcaacgcgc
     5101 ttaccgcgag tcagctcttt cagcttctcg tacggttttt cgatgccata gcgacgcata
     5161 actgtctgga ttggctcagc cagcacttcc cagttgtgat ccagttcatc cagcagatgg
     5221 tcacggttca cttccagttt gctcacccct ttcagggtgg attgatacgc aatcagcgca
     5281 taaccgatac ccacgccgag gttacgcagc acggtggagt cggtcaggtc acgctgccag
     5341 cgggaaaccg gcagtttgct tgccagatgc tgcaataccg cgttggaaag gcccagattc
     5401 ccttcggagt tttcgaagtc gatcgggtta actttatgcg gcatggtgga agaaccaatc
     5461 tcaccagcaa tggttttctg tttgaagtgg ttaagggcga tataacccca gacgtcacgg
     5521 tcaaagtcga tcagaatggt gttgaagcgc gcaacgcaat caaacagctc ggcaatgtag
     5581 tcgtgcggtt cgatctgggt agtgtacggg ttccactgaa tacccagcga ggtgacgaac
     5641 tcttcgctga actgatgcca gtcaacttcc gggtaagcgg cgatgtgggc gttatagtta
     5701 ccgactgcgc cgttgatttt accgaggatc tccacctgat taagctggcg gtactggcgc
     5761 tccatacggt aggcgacgtt agccatctct ttaccgatgg ttgacggcgt ggctggctga
     5821 ccgtgggtac gggacagcag cgggatatcg cgatactgaa cggcgagatc tttaatgcca
     5881 tcaatcagtt gacgccagta cggcaggatc acttcatcac gcgcggtttt cagcatcaac
     5941 gcgtgggaga ggttattgat atcttccgaa gtacaggcaa agtggatgaa ttcagaaacc
     6001 gcatgcagtt ccgggatctc cgccactttt tctttcagga aatactcaac cgctttaacg
     6061 tcgtggttag tggtacgctc gatggttttg atgcgtgctg catcttcttc gctgaaactg
     6121 gcgacaattg catcaaggta accgattgcg tcggcagcaa aagcaggaac ttccttgatc
     6181 gctgcgtgcg cggccagttt ttgcagccaa cgtacttcaa cttgtacacg gaatttcagc
     6241 aaaccatatt cgctgaaaat cccgcgcagc gcgctgactt tatcgccgta gcgtccatcg
     6301 acaggggaaa cggcggtcag tgaggataat tccatagatc acaactccgg ggttaaatga
     6361 gcaagaattt gttttgcctg agtggtcagg cgattacgag aaaacatcag ttgcagacgt
     6421 ccgccgccga cctggtgcca gagcacggcg gcgcgaatgc ctgccagcag ggtagcgcga
     6481 actttcgcct gcacttgtgg gctttgcagt acagcagggg aaccggtgac ctgaatgcgc
     6541 gggccaagcg ggctaatcac atcaacatag atagcagcca tcgcgctcat cagcgtttcg
     6601 gactgtaaat cgaagtgttc gagctggcgt tgcaggccgt tgatccggtt gcccagagtg
     6661 tcgagcgcac ctttcgctga ggagagtttg cgctcaagca ccatcaggct gagtgtgtag
     6721 cgggttaact cagcgtttaa gccctggcga ctgctggcat tgagcacgcc gagcaaggtt
     6781 tccagcccga cgcgcaggtt ggcttcgcta ccgccaaaaa ccgccagcgt cgagctgggg
     6841 ttcatatcaa taatactgtt gagtgagacg tgtagcgcat cggcatcaca atgcccctgg
     6901 tgagcgagtt gttgcaccag gcgtgccgac tgacaaatac cggccagagc gagggtgatg
     6961 tcatagtaat tctttgccac gttcactgct tccttgtttt aagtaaagaa aataatcaga
     7021 ccggcagcgg cagacgctgc tcaataatac cgccaccgag gcacacttca ccgtcataga
     7081 atacggcaga ctggcccggc gtcacggcgg caaccggctc atcgaaaatc acttcaatgc
     7141 gatcatcgtc cagcgccttg acggtgcacg gaatgtcggt ctggcgatag cgggttttta
     7201 ccgtgcaacg catagtgccg gtgaatggtt cgcgatcgac ccagtgcaac tgctgggcaa
     7261 tcagaccgac agacatcaga cgcgggtgtt catggccctg cgcaacaatc agaatgttgt
     7321 tttcgacgtc tttatcgacc acataccacg gttcttcggt gccttctttg gtgccaccaa
     7381 tacccagacc tttacgctga ccgagggtgt gatacatcag cccctggtgt tcaccaattt
     7441 catcgccatc gacggtaatg attttgcccg gttgcgccgg gagataacgg cccaggaact
     7501 cgcggaattt acgttcgcca atgaagcaaa tgccggtaga gtcttttttc ttcgcggtga
     7561 ccagaccaag atcttcagca atcttacgca cctgcggttt ttccagttcg ccgaccggga
     7621 acaggctttg cgcaatctgc tcatggctga gcgtataaag gaagtagctc tggtctttat
     7681 tgctgtccag accacgcagc aggcggctct tgccatcgac atctgcccga cgtacgtaat
     7741 gaccggtagc gatataatcg gcacctaaat cttcggcggc aaattcgagg aaggctttaa
     7801 atttgatctc tttgttgcac agaatatccg gattcggcgt gcgaccggct ttatattcgg
     7861 caaggaacag ttcgaagacg ttgtcccagt actcggcagc aaagttaacg gtgtgcagtt
     7921 caatgccgag cttgtcgcag acagcctggg catcagccag atccgctgcc gctgtgcaat
     7981 attcctcacc gtcgtcttct tcccagttct tcataaacag gccttcgacc tgatatccct
     8041 gttgttgcaa cagccaggca gaaacggagg aatcgacacc gccggacatg ccgacgatta
     8101 ctttttttgc ggtttcagac attggatcac tcacgacatt gaacttcaag gcggcgtatt
     8161 ctatcacgca gccttttaga tgacaccctt tgtaaaaggc cagttaaaat cgccaatcat
     8221 ctccagcgga tagcgttgcc cgctttgata acaacgaata ctttccgcca ccagcggcga
     8281 acgcagattt gacgcctgta aaatttcttc ggcgctgacc caacggcagc agtcgatatc
     8341 gctgtcatga ggctgggtcg ggcatatttg ctcaagctca atagcaaaca ggaaacgcaa
     8401 aaacggcgtt ttatctggcg caatccactg atgcatacga ataaagtgtt gcggctgcgc
     8461 gctgatgccg gtttcttccc acagctcacg cgcagcagct tccactagcg tctcatccgc
     8521 ttccagatgc ccggcaggtt ggttccataa cgctttacca ttaatcgtct cttcaacgac
     8581 taaaaatttg ccttctgcgt gcaccacgca ggcaacggta acgtgcggtt taaacatttt
     8641 gcgtccttaa tctgtcgctt ctcgccattc gccattggca agattatcca aagagtaatc
     8701 acccatcgca tagcgaatca gtcgcagcgt ggggaaacca acatgggcgg tcatgcggcg
     8761 cacctggcga tttcgtcctt catataaggt gatcttcagc cagctggtgg gaatactttt
     8821 gcgttcacga attggcggat tacggggcca taaccattcc ggctcctcaa ccatctctgc
     8881 acctgcgggc agggtagggc catcatttaa ggttacgcca ttgcgcaacg cttcaagggc
     8941 gtcttccgtg ggaatacctt ctacctgtac ataatagatt ttcccggtac gtttacccgg
     9001 ctgggttaaa cgcgcctgca acgcgccgtt attggtcagc accagtaacc cttcgctatc
     9061 gcggtcaagg cgacctgctg cataaacgcc ttgaaccggg atgaattctt ttaatgtttt
     9121 gcgtccggct tcatcggtga actgcggaag aacatcgtag ggtttattga acaggatcac
     9181 acgcgtgggc tggttttcag gtttacgcct ggtagaacgt tgcgagctga atcgcttaac
     9241 ctggtgattt ctaaaagaag ttttttgcat ggtattttca gagattatga attgccgcat
     9301 tatagcctaa taacgcgcat ctttcatgac ggcaaacaat agggtagtat tgacaagcca
     9361 attacaaatc attaacaaaa aattgctcta aagcatccgt atcgcaggac gcaaacgcat
     9421 atgcaacgtg gtggcagacg agcaaaccag tagcgctcga aggagaggtg aatggaaagt
     9481 aaagtagttg ttccggcaca aggcaagaag atcaccctgc aaaacagcaa actcaacgtt
     9541 cctgaaaatc cgattatccc ttacattgaa ggtgatggaa tcggtgtaga tgtaacccca
     9601 gccatgctga aagtggtcga cgctgcagtc gagaaagcct ataaaggcga gcgtaaaatc
     9661 tcctggatgg aaatttacac cggtgaaaaa tccacacagg tttatggtca ggatgtctgg
     9721 ctgcctgctg aaacccttga tctgattcgt gaatatcgcg ttgccattaa aggcccgctg
     9781 accactccgg ttggtggcgg tattcgctct ctgaacgttg ccctgcgcca ggaactggat
     9841 ctctacatct gcctgcgtcc ggtacgttac taccagggca ccccaagccc ggttaaacac
     9901 cctgaactga ccgatatggt tatcttccgt gaaaactcgg aagacattta tgcgggtatc
     9961 gaatggaaag cagactctgc cgacgccgag aaagtgatta aattcctgcg tgaagagatg
    10021 ggcgtgaaga aaattcgttt cccggaacat tgcggtatcg gtattaagcc gtgttctgaa
    10081 gaaggcacca aacgtctggt gcgtgcagcg atcgaatacg caattgcgaa cgatcgtgac
    10141 tctgtgaccc tggtgcacaa aggcaacatc atgaagttca ccgaaggcgc gtttaaagat
    10201 tggggctacc agctggcgcg tgaagagttt ggcggtgaac tgatcgacgg cggcccgtgg
    10261 ctgaaagtta aaaacccgaa taccggcaaa gagatcgtca ttaaagatgt gattgctgat
    10321 gcattcctgc aacagatcct gctgcgtccg gctgaatatg atgttatcgc ctgtatgaac
    10381 ctgaacggtg actacatttc tgacgctctg gctgcacagg tcggcggtat cggtatcgcc
    10441 ccaggcgcaa acatcggtga cgaatgcgcc ctgtttgaag ccacccacgg tactgcgccg
    10501 aaatatgccg gtcaggacaa agttaaccct ggctctatta ttctctccgc tgagatgatg
    10561 ttacgccata tgggctggac tgaagccgca gacctgattg ttaaaggtat ggaaggcgca
    10621 atcaacgcca agactgtaac ctatgacttc gaacgtctga tggaaggcgc taaactgctg
    10681 aaatgcagcg agtttggtga tgcgatcatc aagaacatgt aatctctaca tgtgttaaat
    10741 attgaaacgg gcgtataaca cgcccgtt
//
LOCUS       DAZGAP010000175        10695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGAP010000175 DAZGAP010000000
VERSION     DAZGAP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.176"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..157)
                     /locus_tag="ACHLAM_004147"
     CDS             complement(<1..157)
                     /locus_tag="ACHLAM_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855598.1"
                     /translation="MHTPIGVKPVAGSKEWQEAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLI"
     gene            266..760
                     /gene="napF"
                     /locus_tag="ACHLAM_004148"
     CDS             266..760
                     /gene="napF"
                     /locus_tag="ACHLAM_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO7855599.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNACGACAASCPVSAITAEY
                     LHVH"
     gene            750..1013
                     /gene="napD"
                     /locus_tag="ACHLAM_004149"
     CDS             750..1013
                     /gene="napD"
                     /locus_tag="ACHLAM_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO7855600.1"
                     /translation="MYTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1010..3496
                     /gene="napA"
                     /locus_tag="ACHLAM_004150"
     CDS             1010..3496
                     /gene="napA"
                     /locus_tag="ACHLAM_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO7855601.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3503..4198
                     /gene="napG"
                     /locus_tag="ACHLAM_004151"
     CDS             3503..4198
                     /gene="napG"
                     /locus_tag="ACHLAM_004151"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO7855602.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4185..5048
                     /gene="napH"
                     /locus_tag="ACHLAM_004152"
     CDS             4185..5048
                     /gene="napH"
                     /locus_tag="ACHLAM_004152"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO7855603.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPASVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5045..5494
                     /gene="napB"
                     /locus_tag="ACHLAM_004153"
     CDS             5045..5494
                     /gene="napB"
                     /locus_tag="ACHLAM_004153"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO7855604.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5504..6106
                     /gene="napC"
                     /locus_tag="ACHLAM_004154"
     CDS             5504..6106
                     /gene="napC"
                     /locus_tag="ACHLAM_004154"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO7855605.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6119..6742
                     /gene="ccmA"
                     /locus_tag="ACHLAM_004155"
     CDS             6119..6742
                     /gene="ccmA"
                     /locus_tag="ACHLAM_004155"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO7855606.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAENKIRRISLTQTRGE"
     gene            6739..7401
                     /gene="ccmB"
                     /locus_tag="ACHLAM_004156"
     CDS             6739..7401
                     /gene="ccmB"
                     /locus_tag="ACHLAM_004156"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO7855607.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7443..8180
                     /gene="ccmC"
                     /locus_tag="ACHLAM_004157"
     CDS             7443..8180
                     /gene="ccmC"
                     /locus_tag="ACHLAM_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO7855608.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8177..8386
                     /gene="ccmD"
                     /locus_tag="ACHLAM_004158"
     CDS             8177..8386
                     /gene="ccmD"
                     /locus_tag="ACHLAM_004158"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO7855609.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8383..8862
                     /gene="ccmE"
                     /locus_tag="ACHLAM_004159"
     CDS             8383..8862
                     /gene="ccmE"
                     /locus_tag="ACHLAM_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO7855610.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8859..>10695
                     /gene="ccmF"
                     /locus_tag="ACHLAM_004160"
     CDS             8859..>10695
                     /gene="ccmF"
                     /locus_tag="ACHLAM_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO7855611.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAMLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRNGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRW"
BASE COUNT         2199 a         2735 c         3207 g         2554 t
ORIGIN      
        1 tgatgagaaa acaaaccagc agaaatatct ctggcgaatt aatcgctata taaatatgtt
       61 tataaccatt tgaaatgtga gcaaaagccc gtttttgcca cgcttcctgc cactcttttg
      121 atcctgctac aggttttacc ccgatcgggg tatgcatctt tgacacatcc tttaatatct
      181 tagcggctat aaaaatggct tattaattat gcggtttatt tggtcgctct caattttcag
      241 agcgcgttaa tgatggaagg tcaatgtgaa gattgatgca tcccgtcggg gcatactcac
      301 tggtcgctgg cgcaaagcca gtaacggtat ccgtccgccc tggtccggtg atgaatctca
      361 ttttctgacc cattgcactc gctgcgacgc ctgtattaat gcttgtgaaa acaacattct
      421 gcaacgcggc gcgggtggct atccgagcgt taatttcaaa aataacgagt gcagcttctg
      481 ttacgcctgc gcgcaggctt gccccgaatc gctattttct ccgcgccaca ccagggcctg
      541 ggatttgctg ttcaccatcg gggaggcgtg cctggcgtat cagtcagttg aatgccgccg
      601 ctgtcaggat agctgtgagc caatggcaat tatctttcgc ccgacgctct ccgggatcta
      661 ccagccgcaa cttaatagcc aactttgcaa cgcatgtgga gcatgtgcag ccagctgtcc
      721 ggtatcagcc ataactgcgg agtatctcca tgtacactaa ctggcaagtt tgcagcctgg
      781 tcgtgcaggc caaaagcgaa cgaatttcag acatcagcac ccaactgaac gcctttcccg
      841 gctgtgaagt tgctgtcagc gacgcgccga gcggccagtt gattgtggtg gtggaagcag
      901 aagacagcga aacgctgatc caaaccattg agtcagtacg caacgtagag ggcgtgctgg
      961 cggtgtcgct ggtttatcac cagcaggaag agcaaggtga ggaaacacca tgaaactcag
     1021 tcgtcgtagc tttatgaaag ctaacgccgt tgcggccgct gcggcggctg ccggtctcag
     1081 cgtgccgggc gttgcccgcg ccgttgttgg tcagcaggaa gccattaaat gggataaagc
     1141 gccgtgccgt ttctgcggta cgggttgcgg cgttctggtc ggaacgcagc aggggcgtgt
     1201 ggtggcctgt cagggcgacc cggacgcacc ggttaaccgt ggtctgaact gcattaaggg
     1261 ctatttcctg cccaaaatca tgtacggtaa agaccgtttg acgcagccgc tgctgcgtat
     1321 gaaaaacggt aaatatgaca aagaaggcga atttacccca atcacctggg atcaagcctt
     1381 cgatgtgatg gaagagaaat tcaaaaccgc cctgaaagaa aaagggccgg aatcgatcgg
     1441 tatgttcggt tctggtcagt ggactatctg ggaaggttat gccgcgtcca agctgtttaa
     1501 agcgggattc cgttcgaaca acatcgaccc gaacgcgcgt cactgtatgg cgtcggcggt
     1561 agttggcttt atgcgtacct ttggtatgga tgagccgatg ggctgctatg acgacatcga
     1621 gcaggctgac gcgtttgtgc tgtggggctc caacatggcg gagatgcacc cgatcctctg
     1681 gtcgcgcatc actaaccgtc gtctctccaa ccagaacgtc accgtggcgg tgctttctac
     1741 ctaccagcat cgtagcttcg agctggcgga taacggcatc atctttacgc cgcaatctga
     1801 cctggtgatc ctgaactata tcgccaacta tatcattcaa aacaatgcga taaatcagga
     1861 cttcttcagc aaacacgtta acctgcgcaa aggggcgacg gacatcggct acggtttacg
     1921 tccgacccat ccgctggaaa aagcagcgaa gaatccgggt tctgacgcct ccgaaccgat
     1981 gagctttgaa gattacaaag ccttcgttgc tgaatatacg ctggaaaaaa ccgccgaaat
     2041 gaccggcgtg ccgaaagacc agttagaaca actggcgcag ctgtatgccg atccgaacaa
     2101 gaaagtcatc tcctactgga cgatgggctt caaccagcat acccgtggtg tgtgggccaa
     2161 caacctggtc tacaacctgc acctgctgac cggcaaaatt tcccagccgg gttgcggtcc
     2221 gttctccctg accgggcagc cttccgcgtg tggtactgcg cgtgaagtgg gcacctttgc
     2281 tcaccgtctg cctgcggaca tggtggtgac taacgagaaa catcgtgata tttgcgagaa
     2341 gaagtggaat atcccgagtg gcaccattcc ggcgaaaatc ggtctgcacg cggtggcaca
     2401 agaccgtgcg ctgaaagacg gcaagctgaa tgtttactgg accatgtgta ccaacaacat
     2461 gcaggccggg ccgaacatta atgaagagcg tatgccaggc tggcgcgatc cgcgcaactt
     2521 tatcatcgtt tccgatccgt atccgacagt cagtgcgctg gcagcggact tgatcctgcc
     2581 gaccgcaatg tgggtagaga aagagggggc ttacggtaac gccgaacgcc gtactcaatt
     2641 ctggcgtcag caggtacagg caccgggcga agcgaaatcg gatctctggc agttagtcca
     2701 gttctcccgc cgcttcaaaa ctgaagaagt atggccggaa gagctgctgg cgaagaaacc
     2761 ggaactgcgt ggcaaaacgc tgtacgaagt tctgtatgcc accccggaag tgagcaaatt
     2821 cccgctatcc gaactggcgg aagatcagct gaacgatgaa tcccgcgagc tgggcttcta
     2881 tctgcaaaaa gggctgttcg aagagtacgc atggtttggt cgcggtcacg gtcacgatct
     2941 cgcgccgttc gatgactacc acaaagcacg cggtctgcgc tggccggtgg tgaacggtaa
     3001 agaaacgcag tggcgttaca gcgaaggtaa cgacccgtac gtgaaagcgg gcgaaggcta
     3061 caagttctac ggtaaaccgg atggcaaagc ggtgatcttc gcgctgccgt tcgaacctgc
     3121 ggcggaagca ccggatgaag agtacgacct gtggctctct accggacgcg tcctggagca
     3181 ctggcatacc ggcagtatga ctcgccgtgt accggaactg caccgcgcct tcccggaagc
     3241 ggtcctgttt attcacccgc tggatgcgaa agcgcgcgat ctgcgccgtg gcgacaaagt
     3301 gaaagtggtt tctcgccgtg gcgaagtgat ctcgattgtc gaaacgcgcg gtcgtaaccg
     3361 tccgccgcag ggcctggtgt acatgccgtt cttcgatgcc gcacagctgg ttaacaaact
     3421 gacgctggat gcgaccgatc cgctctcgaa agagacggac ttcaagaagt gcgcggtcaa
     3481 actggagaag gtgtaagcca ttatgtcccg gtcagcgaaa cctcaaaatg gtcgccgccg
     3541 ctttctgcgc gatgtcgttc gcacagctgg cgggctggct gccgtgggtg tggcgctggg
     3601 gttacaacag caaaccgcac gcgcatctgg cgtgcggttg cgcccgcccg gagccataaa
     3661 cgagaacgcc tttgccagtg cctgtgtgcg ttgcggtcag tgtgttcagg cttgccctta
     3721 cgacacctta aaactggcga cgctggcctc tggcctgtcg gcgggcacgc catattttgt
     3781 cgcacgggat attccttgcg aaatgtgtga ggacattccg tgcgccaaag tgtgcccgag
     3841 cggtgcgctg gatcgtgaga tcgaatcgat cgacgacgcg cgaatggggc tggcggtact
     3901 ggtggaccag gaaaactgtc tcaactttca ggggctgcgc tgcgatgttt gttatcgcga
     3961 atgcccgaaa attgatgagg ccatcaccct ggagctggag cgcaacacgc gtaccggtaa
     4021 gcacgcccgc tttctgccga cggttcacag cgacgcctgt accggttgcg gtaagtgcga
     4081 aaaagtgtgc gtgctggaac aaccggcaat caaggtgtta ccgctgtcac tagcgaaagg
     4141 ggagttaggt caccattacc gcttcggctg gctggagggg aacaatggca aatcgtaaac
     4201 gtgacgccgg gcgcgaggcg ctggagaaaa aaggctggtg gcgcagtcac cgttggttag
     4261 tgttacgtcg cctttgtcag ttcttcgtgc tggggatgtt tttgagcggt ccgtggtttg
     4321 gtgtgtggat cttgcacggc aactacagca gtagcctgtt attcgacacc gttccgctga
     4381 ccgatccgct gatgacgctg caaagtcttg ccagcggtca tctgccggcc tcggtggcgc
     4441 tgaccggagc ggtgattatc accgtgctct acgccctggc aggaaagcga ttattttgca
     4501 gctgggtctg cccgctgaac ccgattaccg acttagcgaa ctggttacgc aggcgctttg
     4561 acctcaatca gtctgcgacg atcccacgcc acattcgcta cgtgctgttg gtggtcattc
     4621 tgctgggatc ggccttaacc ggcacgctca tctgggaatg gatcaacccc gtttccctga
     4681 tggggcgcag tctggtaatg ggattcggca gcggcgcgct gcttattctc gcgctgtttt
     4741 tatttgatct actggtcgtt gaacacggct ggtgcgggca catttgtccg gttggcgcgc
     4801 tgtatggcgt gctgggtagc aaaggtgtga ttaccgttgc ggccaccgat cggcagaaat
     4861 gtaaccgctg tatggattgt tttcatgttt gcccggaacc gcatgtgcta cgtgccccgg
     4921 tgctggatga acaaagcccg gtgcaggtca ccagccgcga ttgcatgacg tgcggtcgct
     4981 gcgtggatgt ctgttctgag gatgtattta caataactac acgatggagt tcgggagcga
     5041 aatcatgaaa agccatgacc tgaagaaagc gctgtgtcaa tggacggcga tgctggccct
     5101 ggtggtgagc ggcgcggttt gggcggctaa tggcgtcgat tttagccagt cgccggaagt
     5161 atccgggacg caggaagggg ccattcggat gccaaaagag caggatcgga tgccgctgaa
     5221 ctatgtgaat cagccgccaa tgatcccgca tagcgttgaa ggttatcagg taacgaccaa
     5281 taccaaccgc tgcttgcagt gccacggtgt cgaaagctat cgcaccactg gcgcgccgcg
     5341 tatcagtcct actcacttta tggacagcga cggcaaagtg ggcgcagaag tggctccgcg
     5401 tcgttatttc tgtctgcaat gtcacgtacc gcaggccgat accgcgccaa tcgtggggaa
     5461 tacctttacc ccatcaaaag gttacgggaa ataagaggtt attatgggaa attctgaccg
     5521 taagcctggt ctgattaagc gcctgtggaa atggtggcgt acccccagcc gtctggcgct
     5581 ggggacgctg ctgttgatcg gttttgttgg cggcatcgtc ttctggggcg gctttaacac
     5641 cgggatggaa aaagccaata ccgaagagtt ctgcattagc tgccacgaaa tgcgtaacac
     5701 ggtgtatcag gaatacatgg attccgtgca ctacaacaac cgtagcggcg ttcgtgcgac
     5761 ctgtccggat tgtcacgttc cgcacgagtt tgtgccgaag atgatacgca agctcaaagc
     5821 aagtaaagag ctgtatggta aaatttttgg cgttattgac acgccgcaga aatttgaagc
     5881 tcatcgcctg acgatggcac agaatgagtg gcggcgcatg aaggacaata actcgcagga
     5941 gtgccgtaac tgtcacaact tcgagtatat ggatacaacc gcccagaaat cggttgccgc
     6001 gaagatgcat gaccaggcgg tgaaagatgg gcaaacctgt attgattgcc ataaagggat
     6061 agcgcacaag ctgcccgata tgcgcgaagt cgagccaggt ttttaacagg gttattgcgt
     6121 gggtatgctt gaagccagag agttactttg cgagcgggat gaacgaacct tatttagtgg
     6181 cttgtcattt acgctgaacg caggagagtg ggtacaaatc accggtagca acggcgcggg
     6241 gaagacaacg cttctccgtt tgctgacggg gttgtctcgc cctgatgcgg gtgacgttct
     6301 ctggcaaggg cagcccttgc atcaggtacg cgacagctac catcaaaacc tgttatggat
     6361 agggcatcag ccggggatca aaacccggct gacggcgtta gaaaatctgc acttttatca
     6421 tcgcgatggc gataccgcac aatgtctgga agccctggcg caggccgggc ttgccggatt
     6481 cgaagatatt ccggtaaatc aactttctgc cgggcaacaa cgccgcgtcg ctttagcgcg
     6541 tctgtggctg acccgtgcca cgttatggat cctcgacgag ccttttaccg cgattgacgt
     6601 taacggcgtc gatcgtctga cccagcgtat ggcgcagcat acggagcagg gggggattgt
     6661 gattctgact acccaccagc cgctcaacgt tgctgaaaat aaaattcgcc gcatttcact
     6721 gacgcaaacg aggggcgaat aatgttctgg cgcattttcc gtcttgagct gcgcgtagcg
     6781 tttcgccata gcgccgaaat cgccaacccg ctgtggttct tcctgattgt cattaccctt
     6841 tttccgctca gtatcggtcc ggagccgcaa ctgctggcgc gtattgcgcc gggcattatc
     6901 tgggttgctg cgctgctttc atccttgctg gcactggagc gactgttccg tgacgatttg
     6961 caggacggca gtcttgaaca attgatgttg ttgccgttac ccttgcccgc cgttgtgctg
     7021 gcgaaggtga tggcgcactg gatggtaacc ggtctgccgt tactcatcct ttcgcccctg
     7081 gtagcaatgc tactgggaat ggatgtttat ggctggcaag tgatggcgtt aacgctactg
     7141 ctgggaaccc ctacgcttgg ctttcttggt gcgccgggcg tggcgctgac agtgggactt
     7201 aagcgcggtg gtgtgctgct cagcatactg gtgttaccgc tgactatccc attactcatc
     7261 tttgccaccg ccgcgatgga cgcggcttct atgcatttgc ccgttgatgg gtatctggca
     7321 attttaggcg cgttgctggc aggcaccgcg acattaagtc cttttgcgac ggcggcagcg
     7381 ttacgaatca gcattcaata acgctggatt actgattttt gttcgagtct ggtatcgaaa
     7441 ctatgtggaa aacactgcat caactggcga tcccaccacg gctgtatcaa atctgtggct
     7501 ggtttatacc gtggctggca attgccagtg tggtcgtgct taccgtcggc tggatctggg
     7561 gattcggttt tgctccggct gattatcagc agggaaatag ctaccgcatt atctacctgc
     7621 atgtgcctgc ggcgatctgg tcgatgggca tttatgcatc aatggcggtg gcggcgttta
     7681 ttggccttgt ctggcagatg aaaatggcca acctggcggt ggcggcgatg gcccccattg
     7741 gtgccgtgtt tacctttatt gccctggtta ccggctctgc atggggaaaa ccgatgtggg
     7801 gcacctggtg ggtatgggat gcacgcctga cttctgaact ggtactgctg tttttgtatg
     7861 tgggtgtgat tgccctgtgg cacgccttcg acgaccgccg tctggcgggc cgtgcggcag
     7921 gtattctggt gctgattggc gtggtgaatc tgccgattat tcattactcc gtggagtggt
     7981 ggaacaccct gcatcaggga tcaacgcgga tgcaacaaag tatcgatccg gcgatgcgtt
     8041 cgccgctgcg ctggtcgatt tttggcttcc tgctgctgtc tgccacgctg acgctgatgc
     8101 ggatgcgtaa tttgattttg ctgatggaaa aacgccgtcc gtgggtgagt gaactgatac
     8161 tgaaaagagg ccgtaaatga cccctgcatt tgcttcctgg aatgaatttt tcgcaatggg
     8221 cggttacgcc ttttttgtct ggctggcggt ggtgatgacc gttattccgc tggtgatttt
     8281 ggtcgtgcac tcggtgatgc agcatcgcgc aattctgcgt ggcgtggcgc aacagcgggc
     8341 gcgtgaggcg cgtttacgtg ctgcgcaaca gcaggaggct gcatgaatat tcgccgtaaa
     8401 aaccgcttgt ggattgcctg tgccgtgctg gcggggctgg cgctgactat cggtctggtg
     8461 ctatatgcgc tgcgctcgaa tatcgatctc ttttatacgc cgggggagat tctctacggc
     8521 aagcgtgaaa ctcagcagat gccggaagtc ggtcagcgtc tgcgcgttgg cgggatggtg
     8581 atgccgggga gtgtgcagcg cgatcccaat tcgctaaaag taactttcac catttacgac
     8641 gctgaaggtt cagtagatgt ctcttacgaa ggcattttgc cggatctgtt ccgtgaaggg
     8701 cagggcgttg tggtgcaggg cgagctggaa aaaggcaatc atatcctcgc gaaagaagtg
     8761 ctggcgaaac acgatgaaaa ctacacgccg ccagaagttg agaaagcgat ggaagctaac
     8821 caccgtcgcc cggcgagtgt ttataaggac ccagcatcat gatgccagaa attggtaacg
     8881 ggctgctgtg tctggcgcta ggaattgcgc tgctgctgtc tgtgtatccg ctatggggcg
     8941 tggcgcgcgg ggacgcgcgc atgatggcgt cttcccgctt gtttgcctgg ctgctgttta
     9001 tgtctgtggc tggcgcattt ctggtgctgg tcaatgcttt cgtggtcaac gactttaccg
     9061 tcacctatgt tgccagcaac tccaataccc agcttccggt gtggtatcgc gtggcggcta
     9121 cctggggcgc gcatgaaggc tcgctcctgc tgtgggtgct actgatgagc ggctggacct
     9181 ttgcggtagc gatttttagt cagcgtattc cgctggatat tgtggcccgc gtactggcga
     9241 taatggggat ggtcagtgtc ggctttttgc tgttcattct ctttacctct aacccgttct
     9301 cacgcacgct gccgaatttc ccgattgaag ggcgcgatct taacccgctg ttgcaggatc
     9361 cggggctgat cttccatccg cctctgctct atatggggta cgtcggtttc tcggtggcgt
     9421 ttgctttcgc cattgcttct ttgttgagtg ggcgtctgga cagcacctat gcgcgtttta
     9481 cccgcccgtg gacgctggcg gcgtggatct tcttgacgct cggcattgtg ctcggttccg
     9541 catgggccta ttacgaactc ggctggggcg gctggtggtt ctgggatccg gtagaaaacg
     9601 cctcgtttat gccgtggctg gtggggactg cgctgatgca ctcactggcg gtcactgaac
     9661 aacgcgccag cttcaaagcg tggacattac tgctggcaat cagtgccttc tcgttgtgtc
     9721 tgttggggac tttcctcgtg cgttccggcg tgctggtatc ggtacacgcg tttgcgtctg
     9781 atccggcgcg cggtatgttt atcctcgcct ttatggtgtt ggtgattggc ggttcgctgc
     9841 tgctgtttgc cgcgcgtgga cacaaagttc gctcacgcgt aaacaatgcg ctgtggtcgc
     9901 gggaatctct gctgttagcg aacaacgttt tgctggtcgc cgcgatgctg gtggtattac
     9961 tggggacgct gctgccgctg gtgcacaagc aactgggact gggcagtatt tcgattggcg
    10021 aaccgttctt caacaccatg tttacctggc tgatggtgcc gtttgcgctg ctgcttggtg
    10081 tcggtcctct ggtgcgctgg gggcgcgatc gcccacgtaa aatccgcaat ttattgatta
    10141 tcgccttcat ctcaacgctg gtgctgtcgc tgctgctgcc gtggctgttc gaaagcaaag
    10201 ttgtggcgat gacggtgctc ggcctggcaa tggcctgctg gattgcgatg ctggcaattg
    10261 cggaagctgc gctgcgtatt tcacgtggca cgaaaaccac cttcagttat tggggaatgg
    10321 tggcggctca cctggggctg gcagtgacaa ttgttggtat tgcctttagc cagaactata
    10381 gcgttgagcg tgatgtgcgc atgaagtccg gcgatagcgt cgatattcat gaatatcgct
    10441 tcaccttccg tgatgtcaaa gaggtgactg gtccgaactg gcgtggcggt gtggcgacta
    10501 tcggcgtaac gcgaaatggc aagccggaaa cagtgctgta tgcggaaaaa cgttattaca
    10561 acactgccgg gtcgatgatg accgaagcgg caattgacgg cggcatcacg cgtgacctgt
    10621 acgcggccct cggtgaagag ctggaaaacg gcgcgtgggc cgtgcgtctt tactacaaac
    10681 cgtttgttcg ctgga
//
LOCUS       DAZGAP010000176        10390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGAP010000176 DAZGAP010000000
VERSION     DAZGAP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.177"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            221..1615
                     /gene="galP"
                     /locus_tag="ACHLAM_004161"
     CDS             221..1615
                     /gene="galP"
                     /locus_tag="ACHLAM_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO7855612.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1692..2189
                     /locus_tag="ACHLAM_004162"
     CDS             1692..2189
                     /locus_tag="ACHLAM_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO7855613.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2284..2991
                     /gene="endA"
                     /locus_tag="ACHLAM_004163"
     CDS             2284..2991
                     /gene="endA"
                     /locus_tag="ACHLAM_004163"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO7855614.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3071..3802
                     /gene="rsmE"
                     /locus_tag="ACHLAM_004164"
     CDS             3071..3802
                     /gene="rsmE"
                     /locus_tag="ACHLAM_004164"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO7855615.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3815..4765
                     /gene="gshB"
                     /locus_tag="ACHLAM_004165"
     CDS             3815..4765
                     /gene="gshB"
                     /locus_tag="ACHLAM_004165"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO7855616.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4874..5437
                     /locus_tag="ACHLAM_004166"
     CDS             4874..5437
                     /locus_tag="ACHLAM_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO7855617.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5437..5853
                     /gene="ruvX"
                     /locus_tag="ACHLAM_004167"
     CDS             5437..5853
                     /gene="ruvX"
                     /locus_tag="ACHLAM_004167"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO7855618.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6027..7007)
                     /locus_tag="ACHLAM_004168"
     CDS             complement(6027..7007)
                     /locus_tag="ACHLAM_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO7855619.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            7025..7729
                     /gene="yggS"
                     /locus_tag="ACHLAM_004169"
     CDS             7025..7729
                     /gene="yggS"
                     /locus_tag="ACHLAM_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO7855620.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLDVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7747..8313
                     /gene="yggT"
                     /locus_tag="ACHLAM_004170"
     CDS             7747..8313
                     /gene="yggT"
                     /locus_tag="ACHLAM_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO7855621.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8310..8600
                     /gene="yggU"
                     /locus_tag="ACHLAM_004171"
     CDS             8310..8600
                     /gene="yggU"
                     /locus_tag="ACHLAM_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO7855622.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8608..9201
                     /locus_tag="ACHLAM_004172"
     CDS             8608..9201
                     /locus_tag="ACHLAM_004172"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO7855623.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLIYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9194..10330
                     /gene="hemW"
                     /locus_tag="ACHLAM_004173"
     CDS             9194..10330
                     /gene="hemW"
                     /locus_tag="ACHLAM_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO7855624.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2442 a         2729 c         2726 g         2493 t
ORIGIN      
        1 cagctttcac agttgttaca tttcttttca gtaaagtctt aattgcagat aacagcgttt
       61 aatctatgat gatataactc aattattttc atgcacttaa atcataacca agataaatgt
      121 tagtgtaagc gattacactg atgtgatttg cttcacatct ttttacgtcg tactcaccta
      181 tcttaattca caataaaaaa taaccatatt ggagggcatc atgcctgacg ctaaaaaaca
      241 ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc cttgccgctc tggcgggatt
      301 actctttggc ctggatatcg gtgtaattgc tggcgcactg ccgtttattg cagatgaatt
      361 ccagattact tcgcacacgc aagaatgggt cgtaagctcc atgatgttcg gtgcggcagt
      421 cggtgcggtg ggcagtggct ggctctcctt taaactcggg cgcaaaaaga gcctgatgat
      481 cggcgcaatt ctgtttgttg ccggttcact gttctctgcg gctgcgccaa acgttgaagt
      541 actgattctt tcccgcgttc tgctggggtt ggcggtgggt gtggcttctt ataccgcacc
      601 gctgtacctc tctgaaattg cgccggaaaa aattcgcggc agcatgatct cgatgtatca
      661 gttgatgatc actatcggga tcctcggtgc ttatctttct gataccgcct tcagctacac
      721 cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg gcaattttgc tgctgattgg
      781 tgtcttcttc ctgccagaca gtcctcgttg gtttgccgcc aaacgccgtt ttgttgatgc
      841 cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa gcgaaacgcg aactggatga
      901 aatccgtgaa agtttgcagg ttaaacagag tggctgggcg ctgtttaaag agaacagcaa
      961 cttccgccgc gcggtgttcc ttggcgtact gttgcaggta atgcagcaat tcaccgggat
     1021 gaacgtcatc atgtattacg cgccgaaaat cttcgaactg gcgggttata ccaacaccac
     1081 cgagcaaatg tgggggacgg tgatcgtcgg cctgaccaac gtactcgcca cctttatcgc
     1141 aatcggcctt gttgaccgct ggggacgtaa accaacgcta acgctgggct tcctggtgat
     1201 ggctgctggc atgggcgtac tcggtacaat gatgcatatc ggtatccact ctccgtcggc
     1261 gcagtatttc gccatcgcca tgctgctgat gtttattgtc ggttttgcca tgagtgccgg
     1321 tccgctgatt tgggtactgt gctccgaaat tcagccgctg aaaggccgcg attttggcat
     1381 cacctgctcc accgccacca actggattgc caacatgatc gttggcgcaa cgttcctgac
     1441 catgctcaac acgctgggta acgccaacac cttctgggtg tatgcggctc tgaacgtact
     1501 gtttatcttg ctgacattgt ggctggtacc ggaaaccaaa cacgtttcgc tggaacatat
     1561 tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaatc ggcgctcacg attaatctcc
     1621 tcaggcttcc tcccgtcgcg gaggaagcca cctcttgcag tcatcttttc ttcgctctat
     1681 cctctgccgc tatgaaaaca tcccgtctcc ctatcgccat ccagcaggcc gttatgcgcc
     1741 gcctgcggga aaaactcgcc caggccaacc tgaagctagg gcgtaactac ccggagccaa
     1801 aactctctta cacccagcgc ggaacctccg ccggaacagc ctggctggaa agctatgaaa
     1861 tccgcctcaa tcccgttttg ctgttggaaa acagtgaagc ttttattgaa gaagtggtac
     1921 cgcacgaact ggcacatttg ctggtatgga aacatttcgg ccgcgtagcg ccacatggca
     1981 aagagtggaa gtggatgatg gaaagcgtgc tgggcgttcc cgcccgtcgt acgcatcagt
     2041 tcgaactgca atccgtgcgt cgcaacacct tcccctaccg ctgcaagtgc caggagcatc
     2101 agcttaccgt acgccgccat aatcgcgtag ttcgtggcga ggccgtctat cgctgtgttc
     2161 actgcggtga acagctggtt gcgaaataac cacctgaact atcaggaact ttcctgatct
     2221 ggctgattgc ataccaaaac aactttcgct acgttgctgg ctcgttttaa cacggagtaa
     2281 gtgatgtacc gttatttgtc tattgctgcg gtggtactga gcgcagcatt ttccggcccg
     2341 gcgttggccg aaggtatcaa tagtttttct caggcgaaag ccgcggcggt aaaagtccac
     2401 gctgatgcgc ccggtacgtt ttattgcgga tgtaaaatta actggcaggg caaaaaaggc
     2461 gttgttgatc tgcaatcgtg cggctatcag gtgcgcaaaa atgaaaaccg cgccagccgc
     2521 gtagagtggg aacacgtcgt tcccgcctgg caattcggtc accagcgcca gtgctggcag
     2581 gacggtggac gtaaaaactg cgccaaagat ccggtctatc gcaagatgga aagcgatatg
     2641 cataacctgc agccgtcagt cggtgaggtg aatggcgatc gcggcaactt tatgtacagc
     2701 cagtggaatg gcggcgaagg ccagtacggt caatgcgcca tgaaggtcga tttcaaagaa
     2761 aaagccgccg aaccaccagc gcgtgcacgc ggtgccattg cgcgcaccta cttctatatg
     2821 cgtgaccaat acaacctgac actctctcgc cagcaaacgc aactgttcaa cgcatgggac
     2881 aagatgtatc cggttaccga ctgggagtgc gagcgcgatg aacgcatcgc gaaggtgcag
     2941 ggcaatcata acccgtatgt gcaacgcgct tgccaggcgc gaaagagcta acctacacta
     3001 gcgggattct ttttgttaac ccctacccca cgcgtacaac cgcgtgggga gacgacgcgg
     3061 atttttaact atgcgtatcc cccgcattta tcatcctgaa ccactgacca gccattctca
     3121 catcgcgctt tgcgaagatg ccgccaacca tatcgggcgc gtactgcgta tggggccggg
     3181 gcaggcgttg caattgtttg acggtagcaa ccaggtcttt gacgccgaaa ttaccagcgc
     3241 cagcaaaaaa agcgtggaag tgaaggtact ggaaggccag atcgacgatc gcgaatctcc
     3301 gctgcatatt cacctcggtc aggtgatgtc gcgtggtgaa aaaatggaat ttacgatcca
     3361 gaaatcgatc gaactcggtg taagcctcat tacgccactt ttttctgagc gctgcggcgt
     3421 taaactggat agtgaacgtc tgaacaagaa gctccagcag tggcagaaga ttgcaattgc
     3481 tgcctgtgag cagtgtggtc gtaaccgggt gccggaaatc cgtccagcga tggatctgga
     3541 agcctggtgt gcagagcagg atgaaggact gaaactgaat cttcacccgc gcgccagtaa
     3601 cagcatcaat acgttgccgt taccggttga acgtgtccgc ctgctgattg gcccggaagg
     3661 cggtttatcg gcagatgaaa ttgccatgac tgcccgctat caatttactg atatcctgtt
     3721 gggacctcgc gttttgcgta cagagacaac tgcgctcacc gccattaccg cgctacaagt
     3781 acgatttggc gatttgggct aacggagaag aataatgatc aagctcggca tcgtgatgga
     3841 ccccatcgca aacatcaaca tcaagaaaga ttccagtttc gctatgttgc tggaagcaca
     3901 gcgtcgtggt tacgaacttc actatatgga gatgggcgat ctgtatctga tcaatggcga
     3961 agcccgcgcc catacccgca cgctgaacgt gaagcagaac tacgaagagt ggttttcatt
     4021 cgtcggtgaa caggatctgc cgctggccga tctcgatgtg atcctgatgc gtaaagaccc
     4081 gccgtttgat accgagttta tctacgcgac ctatattctg gaacgtgccg aagagaaagg
     4141 gacgctgatc gttaacaagc cgcagagcct gcgcgactgt aacgagaaac tgtttaccgc
     4201 ctggttctct gacttaacgc cagaaacgct ggttacgcgc aataaagcgc agctaaaagc
     4261 gttctgggag aaacacagcg acatcattct taagccgctg gacggtatgg gcggcgcgtc
     4321 gattttccgc gtgaaagaag gcgatccaaa cctcggcgtg attgccgaaa ccctgactga
     4381 gcatggcact cgctactgca tggcgcaaaa ttacctgcca gccattaaag atggcgacaa
     4441 acgcgtgctg gtggtggatg gcgagccggt accgtactgc ctggcgcgta ttccgcaggg
     4501 gggcgaaacc cgtggcaatc tggctgccgg tggtcgcggt gaacctcgtc cgctgacgga
     4561 aagtgactgg aaaatcgccc gtcagatcgg gccgacgctg aaagaaaaag ggctgatttt
     4621 tgttggtctg gatatcattg gcgaccgtct gactgaaatt aacgtcacca gcccaacctg
     4681 tattcgtgag attgaagcag agtttccggt gtcgatcacc ggaatgttaa tggatgccat
     4741 cgaagcacgt ttacagcagc agtaacccac cttagcgaga aggatctcgt tgagactctg
     4801 agtgacagcg cccttctttc cacgcatact gggcgctgtt gcttttttga accaggaaac
     4861 agaacctctg acaatgaatt tacagcatca ctttcttatt gccatgcctg ctctccagga
     4921 tccgattttc cgtcgttccg tggtctacat ttgcgaacat aataccaatg gtgcaatggg
     4981 gatcatcgtc aacaagccgc tggaaaatct caaaattgaa gggattctgg aaaagctgaa
     5041 gatcacgccg gagccgcgtg atgaatcaat ccgtctggat aaaccggtta tgctcggtgg
     5101 tccgctggct gaagatcgcg ggtttatttt gcatactccg ccctctaatt ttgcttccag
     5161 cattcgcatt tcagacaaca cggtgatgac cacttcccgc gatgtgctgg aaacgctcgg
     5221 caccgataaa caaccgtctg acgtattggt ggctctgggc tatgcctcct gggagaaagg
     5281 tcaactggaa caagaaattc tcgataacgc gtggctaacg gccccggcgg atctgaatat
     5341 tctgttcaaa acaccgattg ccgaccgctg gcgcgaggcg gcaaaactga ttggtgtgga
     5401 tattctcacc atgcctggtg tggcagggca cgcctgatga gtggaacctt actcgccttc
     5461 gacttcggta ccaaaagcat tggtgtagcg gtcggccaac gcattaccgg caccgctcgc
     5521 ccattgcctg caattaaagc acaggacggt acgccggact ggaacattat cgagcgttta
     5581 ctgaaagagt ggcagcctga cgaaatcatc gtcggtttgc cgctgaatat ggacggcacc
     5641 gaacagccgt taaccgccag agcgcgtaaa tttgccaacc gtattcatgg tcgtttcggt
     5701 gttgaagtaa agctccatga cgagcgtctt agcaccgtgg aggcacgttc cggtctgttt
     5761 gaacagggcg gctatcgggc gctcaacaaa ggcaaagtcg actctgcctc tgcggttatt
     5821 attctcgaaa gctatttcga gcagggatat taaggcgatt tactagcgca cagatttctg
     5881 acaatgtgct cgttgtttca tgttggatgc ggcgtaaacg ccttatccaa cctacagtga
     5941 gtgcgaattc aaagttacgc ttcccttgta ggcccgataa gcttgcgcat cgggcatatt
     6001 tacaattcgt atcagacaca gcaacgtcac aagcgccctt ctttcacccg ctgctgataa
     6061 ctctgctgaa acgttatcat ccccacctgc tgcccggttt gaataacatg cggtaactgg
     6121 tgggttttcc cttcacgaat caaattcccc accgcgggtg tgttaatcag caattcaaac
     6181 aacgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac cgcccgtaaa
     6241 ctacctgcca gttgattacg cacgggatct ttttcctgcg ccggaaatga atctaccagt
     6301 cgctcaactg cctgcgccgc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt
     6361 tctgccgccg tcagcgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc
     6421 acatcaggat cttcccgcaa tgcagcccgc aatcccgatg cgaacgtcat acaatgcaaa
     6481 ccgatttccc gctgctggat caaacatcgc tggcttgcat agagatattc gacaggatct
     6541 tccagcgtca gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc
     6601 gtggtagatt tgccgctccc cgtcgccccc gtcaccagaa tcaggccatt ctcgctcttg
     6661 agtaattccg gcaataccgg tggcgcacca agctgttcga gctgtggaca gtgcgaaggt
     6721 aacaaccgta atgccagaga aataccttgc cgttgcgcga acgcactgcc acgcaaccgc
     6781 tggttttccg ccagcgacac agcaaaatcc aactgaccat tctccagcaa tattgtccgc
     6841 tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccggcgcatc aaacggcgca
     6901 gcttccattc gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc
     6961 gagacgttat gctttacact aagggccaca atttcttcca tattcatact aagatcctcg
     7021 gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc
     7081 aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc
     7141 tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt
     7201 tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg
     7261 gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg
     7321 gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga
     7381 acttccccct cttgacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg
     7441 gattcaactg gcagaactgg acgagctggc agctgcggtc gctgaactac cgcgtttacg
     7501 tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt
     7561 tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac
     7621 gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt
     7681 tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg
     7741 aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg
     7801 ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag
     7861 tttgtagtga aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg
     7921 ggacccattg acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc
     7981 gtgctgttta aagtggtgac cttcctgcca atcatctgga ttgccggttt actgattctg
     8041 ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg
     8101 gtaagccagg gtcgtagtcc gattgaatac gtgctgattc agcttgccga tccgctgctg
     8161 cgcccgattc gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc
     8221 gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac
     8281 atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc
     8341 tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg
     8401 gcgacgaggt aaaagtcgcc attaccgcgc cgccggttga cggccaggcc aacagccatc
     8461 tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag
     8521 gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa
     8581 tcgcggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc
     8641 ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa
     8701 acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt
     8761 ctgaaagcgc gccatgcggc aaaagtgacc ggtttaccgg caattgccga cgactccggt
     8821 ctggcggtag atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa
     8881 gacgcgaccg atcaaaagaa cctgcaaaaa ctgctggaaa caatgaaaga cgtaccggac
     8941 gaccaacgtc aggcgcgttt ccactgcgtg ctgatatatc tgcgtcacgc ggaagatccg
     9001 actccgctgg tgtgccacgg tagctggccg ggcgtgatta ctcgtgaacc agcgggtact
     9061 ggtggctttg gttatgatcc aattttcttc gttccttctg aagggaaaac cgctgccgaa
     9121 ctgacccgcg aagaaaagag cgccatttcc caccgtggtc aggcattgaa actgctgctg
     9181 gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg
     9241 cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca
     9301 cgacgattat gttcagcatc tgcttaacga tctggacaac gatgtggctt acgctcaggg
     9361 ccgtgaagtg cagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc
     9421 gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat
     9481 tacgatggaa gcgaacccag gcacggtaga agcggatcgc tttgtcgatt atcagcgtgc
     9541 tggtgtgaac cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact
     9601 cgggcgtatt catggcccgc aagaagcgaa acgagcggca aagctggcga gcggtttagg
     9661 gttacgtagt tttaaccttg atttgatgca tgggctgccg gatcaatcac tggaagaggc
     9721 gctaggcgat ctgcgccagg ctattgaact gaatccgcca catctttcct ggtatcaact
     9781 gaccatcgaa cctaatacgc tgtttggctc gcgccctcct gtactgccgg acgatgacgc
     9841 gctgtgggat attttcgaac aggggcatca gttattaacc gcagcgggct atcagcagta
     9901 tgaaacatcc gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg
     9961 atttggcgac tacatcggta ttggctgcgg cgcgcacggc aaagtgacct tcccggatgg
    10021 gcgcattctg cgtaccacca aaacgcgtca tccgcgtggt tttatgcagg gaagatatct
    10081 ggaaagccag cgtgatgtcg aagctgcaga taaaccgttt gagttcttta tgaatcgctt
    10141 ccgtctgctt gaggccgcgc cgcgcgtgga gtttagtcag tatactggcc tttctgaaga
    10201 agttattcgc tctcagttag acgaggctat cgctcagggt tatctcacag aatgtgcgga
    10261 ttactggcag ataaccgagc atggtaaatt attcttaaac tcacttcttg aattattcct
    10321 cgctgagtaa tcattaatgc gcctgcgggc gcattaatca cttcttttac ccccccccgt
    10381 taattaactt
//
LOCUS       DAZGAP010000177        10378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGAP010000177 DAZGAP010000000
VERSION     DAZGAP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.178"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(185..1132)
                     /gene="dusC"
                     /locus_tag="ACHLAM_004174"
     CDS             complement(185..1132)
                     /gene="dusC"
                     /locus_tag="ACHLAM_004174"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO7855625.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(1194..2387)
                     /locus_tag="ACHLAM_004175"
     CDS             complement(1194..2387)
                     /locus_tag="ACHLAM_004175"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO7855626.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2402..3046)
                     /gene="maiA"
                     /locus_tag="ACHLAM_004176"
     CDS             complement(2402..3046)
                     /gene="maiA"
                     /locus_tag="ACHLAM_004176"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO7855627.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3054..3755)
                     /locus_tag="ACHLAM_004177"
     CDS             complement(3054..3755)
                     /locus_tag="ACHLAM_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO7855628.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3771..4799)
                     /gene="gtdA"
                     /locus_tag="ACHLAM_004178"
     CDS             complement(3771..4799)
                     /gene="gtdA"
                     /locus_tag="ACHLAM_004178"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO7855629.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4811..6169)
                     /locus_tag="ACHLAM_004179"
     CDS             complement(4811..6169)
                     /locus_tag="ACHLAM_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7855630.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            6296..7204
                     /locus_tag="ACHLAM_004180"
     CDS             6296..7204
                     /locus_tag="ACHLAM_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7855631.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7335..7733
                     /gene="yohJ"
                     /locus_tag="ACHLAM_004181"
     CDS             7335..7733
                     /gene="yohJ"
                     /locus_tag="ACHLAM_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO7855632.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7730..8425
                     /gene="yohK"
                     /locus_tag="ACHLAM_004182"
     CDS             7730..8425
                     /gene="yohK"
                     /locus_tag="ACHLAM_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO7855633.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            8555..9439
                     /gene="cdd"
                     /locus_tag="ACHLAM_004183"
     CDS             8555..9439
                     /gene="cdd"
                     /locus_tag="ACHLAM_004183"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO7855634.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALVAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9589..10308
                     /gene="sanA"
                     /locus_tag="ACHLAM_004184"
     CDS             9589..10308
                     /gene="sanA"
                     /locus_tag="ACHLAM_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO7855635.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10311..>10378
                     /locus_tag="ACHLAM_004185"
     CDS             10311..>10378
                     /locus_tag="ACHLAM_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO7855636.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREA"
BASE COUNT         2347 a         2884 c         2696 g         2451 t
ORIGIN      
        1 cttatccctt acggctaccg ggtcgggcgc gaggtaacat ccctgtaaaa cgcgccctca
       61 gcccacatcc atgtgggctg ccccggcctt cagggaacgc ctcggcgatt ttgacgccac
      121 caaacaaccg tgcggcctat tgataaagag ctaacacatt gtcaaaaaac atcactatgg
      181 tttcttagag tttctcgata tcaattgcct gaatagccct tgcaatatca ggggaattat
      241 tcaacacccg aacatgctga aataattccg ttgcttcatc gtattcttta cgcaaataac
      301 tcaaccactg tttaatccgc gcaacgtgat ataacccggt atcgccctgc ttttccagac
      361 gggtatattt ttgcagcaaa gcaactacct ccggccacgg cattcgcggt tcgttatatt
      421 ttaccacccg gctcaggttg ggaatattga gcgccccgcg accaatcatc actgcgtcgc
      481 atccgctgat cgtcatgcat tgttgcgcac tctgccagtc ccagatttca ccgttggcaa
      541 tcacaggaat attcaaccgc tggcgaatct cgccaatcgc ctgccagtca atatgctccg
      601 cgcggtaacc ctgctctttt gtccgcccat gtactaccag ctccgtagcg ccagcctgtt
      661 gcaccgcatc ggcgatttca aatttcttct caccgctgtc ccagcccaga cgcactttta
      721 cgctgacagg caaatgcgcc ggtacagctt cgcgcatcgc ttttgcaccc tgatagatga
      781 gttcaggatc tttgagtaac gtcgccccac cgccgctacc gttaaccgtt ttcgacgggc
      841 agccgcaatt gagatccacg ccccaggaac ctaactcgac cgcacgggcg gcattctctg
      901 ccagccattg tgggaactga cctaacaact gcacgcgcac cagcgtaccg gctggtgtcc
      961 ggctggcgtt ttgtagctca gggcaaatgc gatgaaagac ttttaccggc agcagttgat
     1021 ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt
     1081 cacgcaccag agagtcgagt acaccctcca tcggtgccag taacacacgc atatcatcac
     1141 ccgcaaaaaa atgaggcgct atgttagcgc ctatggtcag cggattaaag tactcagcgt
     1201 ggttgcaaac agttatcttc tttccagccg tacagccact ccatgccgcg gtaaaactct
     1261 gcctgcgatt tccctttcca caacaggtta cgtacctggc gctctactcc tgcggcatga
     1321 taaacccgcc ccatttcacg ggtcgaccag acgatgcgcg cggtgcgcgg aatacgtacc
     1381 gactcataca gcgcaaaggc ttgtgccgca tcaccctcac actgcgccag cgccttaccc
     1441 agtgttaccg cgtcttccag cgccatacag gcaccctgcg ccatatactg cgccaccgga
     1501 tgggccgcat cgcccaccag cgtaatgcgc ttcgttcccc acttcgctac cggttcacgg
     1561 tcggcggttg accagcgtcg ccaggaggtc ggtttatcca gcatctggcg cggacggggg
     1621 tgaatccctt caaagtaaga gagcacttcc tctttactgc catccttcac gccccactct
     1681 tcctgctggc ggctgtggaa tgtcaccacc aggttatact gcttgccgcc gcgtaaaggg
     1741 taatgcacca agtgacagca cggccccgcc cacagtactg gtgcgttaat gcgcaaatct
     1801 tccggcatat cggcacaatc gataacagca cggtagacca catgcccggt gacacgcggc
     1861 gcatcgccca ataagctctg gcgcaccact gacttcacgc cgtcacagcc aatcagaata
     1921 tcgccgatcc aactattgcc gcgatcgtcg aaaacggtga catcgtcttc tgtctcgcga
     1981 atatccacga cgttggtgga ggtgtgatat tccacgtccg gatgcgtcag taccgcttcc
     2041 cacaccgagg cgtgaatatc aacccggtgg atcaccgcgt aagggccgcc gaaatgatcg
     2101 cgaaaggcct gaccggtttc aatgcgcacc acttcttcgg cgtttaccgc atccatcatg
     2161 gtgatgtgat cggtgaatac cgcgcgctgg cgggcgattt cgccgacgcc gaggctatcc
     2221 aacgccgaaa acgcatttgg ccccaactgg atacctgcgc caatctcacc gatctcatgc
     2281 gccttttcca gcagcaccac tttgatcccc tggcgggcca gtgacaatgc agttgccgca
     2341 ccgccgatcc cgcctcccac aattactgcg cgtgtcactt tagtcattat ggttctcctg
     2401 gtcaggctga aattttatct tgttgatttt ctggcgctgc ggcaatgaac gctggcagct
     2461 gcgtgcaggc gtcgtatacc gctttgcagc gcggataccc gctcagatcg cagttcatac
     2521 gtagcgcgtt cgcccactgt ggaacaaggc agcaatctgc cagcgtcggc gtctcgccca
     2581 cacaaaattg ccccgactgg ctctggcgca gtagctgttc caccgcgctt aacccttgct
     2641 ggatccagtg cgcataccag cgttttttat cttcttcact gacgttcagc tcttcggtca
     2701 ggtagcgcag cacgcgtagg ttattcaatg gatgaatatc gcaggcgatg gcatagacaa
     2761 tttccagcac cttgttgcgg gccggttctt cctgcggcat cagcggaact tgcgggtagt
     2821 gactctccag ccagtcaata atcgccagcg attgtccgag tgattcgccc tcgtcggtca
     2881 gcagcgtcgg caccagcccg accggattca tccgccggta ggccagttcg ttttgctgac
     2941 caatgcggat attcacccct tcggtctggt aatcgatacc cttcagcgcc agcgcaatgc
     3001 gcacccggta agacgctgaa ctattaaaaa aactgtacag cttcatcatc acctcagaca
     3061 atcttcaccg cgataggcgt taagccctcg acgttgcccg tgatcacatc acctttgact
     3121 actgagccaa ccccttccgg cgtaccggta aagattagat cgcccggttg cagttcaaag
     3181 aagcctgaca ggtagctgat ggtttcattc actgaccaga tgagatgacg aatatcgctg
     3241 cgctgatgat cttcgccgtt aacctgtaac cagataggtg cgttatcaat accgccagtt
     3301 tcgctggctt tgtgcagcgg cgcgatgggg gccgagagat cgaacgcttt gccgatttcc
     3361 cacgggcggc ccatctggcg catttccatt tgacggtcac ggcgagtcat atcaagaccc
     3421 gtggcataac cccagacata ttcatgggcg ttttccagcg ggatatcact gcctttttta
     3481 ccaatcgcca ccaccagttc aatttcataa tggtagttat cggtctgcgc cggatacggc
     3541 agcgccagcg tttcgccagc ggcaaccgga acaatggcat ccgccggttt gcagaagaag
     3601 aatggcggtt cacgatccgg atcaaagccc atttcgcggg cgtgggcggc atagttacgt
     3661 ccgacgcagt aaacgcggcg tacggggaat tgttcatcac tgccaacaac cggtacggta
     3721 acgggcgctt gtggttcgaa tacgtattga gtcattttct ctctcccaaa ttaataacgt
     3781 gcttcgcgga acagccccag ggcttcttgt accggtctgt ccgaaaaact aaataaaacg
     3841 gtatcttgcg tggtctggaa cgacacgccg tgccaggtcg gcaccacgaa gatatctttt
     3901 gcactaaagc tgaaggtttc attgccgata gtgacctgcc cgctaccttc caccacatgg
     3961 tagatggtgc tgtcagtggt acgcgccacg cgtgaggcaa accctttcgg cagcagttgc
     4021 agaaaagcgc ccatcgacgg catcggatag ccaccggtga ccgggttgac gtagcgcatt
     4081 ttgtagccat cccactcatc ggcatcgccc aggcgggtta aatcgtgtag cacttcgcgg
     4141 ctacggtcat aacggtagtt aaagatgggc gaggagttcc cggtctgatg gcgcaacggc
     4201 aacatattgg cagcgtaacg cggcaggtaa tcgccctctt tacgcgttac tggttgttgc
     4261 tcctccgggt aatcttcggc aaaaccgcag cccagagtgt tcactaatgg cagatccagc
     4321 ccatcgagcc agataaccgg ttcgtcgcca gggttaccgt gatcgtgcca gcgccactgc
     4381 ggggtcagga taaaatcgcc ctcattcatt gacgtgcgtt cgccatcaac ggcggtgaat
     4441 gcccctttac cttcaacaat aaaacgcagc gccgactggt tatggcgatg actcggtgcc
     4501 acttcgcccg gcatgatcag ttgcaaaccg gcatataaag tcgcggtgat ggaagactgc
     4561 ccgcgcagcg ccgggttttc cagcaccagc acgcggcgca cggcttcttt cgcgccaatc
     4621 aggctgccgc tctccagcag caacgggcgg atctcctgat aattccagta cgctggcgca
     4681 cagttagcgt tgggcgtttt tggcaccaga tggtgcagtg actcccacag cggggtcagg
     4741 ttttgcccgg aaatatgctg ataaaattgc tcgcggctat tttgattgtt atcagtcatt
     4801 gggatgctcc ttattcgttc acaacacctg cgtgaggcag tgagtcaggg acagaggtcg
     4861 actggcgaac cacggtgatc agctgggtta acatcaccgc actgatcgcc gcgggcacag
     4921 cgataatgaa aaacagggtg tcgaaagaga aattcatcgc catcataacg ccgccagaca
     4981 gcgagccaac aatcgcgccg cagcggccca cggcgtttga ccagctcacg cccgtggcgc
     5041 gactttgtgt tggataaagt gttgcggtca gcgcattcag ccccacctgc gatccgctga
     5101 tgccaatgcc ggtgccgaaa atcgccagcg ccattagcca taatccgtcc tggcttaagc
     5161 caatcatcac gatgcataca gcgcctatgg catagctcag cgccagtacg cggaaaggat
     5221 tgaacttatc cataagcacg cccagcgcca gcgcgcccaa cgtaccgcca atctggaacg
     5281 cggcggtaac ccaggaggca tgttgcaggt cgatgccgcg atggttgagc agcgttggca
     5341 tccagcttga gagcaggtaa ataatcagca agctcataaa gaacaccacc cacagcatca
     5401 acgtgatggg taactgacgc cccataaaca gttgacgaat gctgcctttt gtaacggagg
     5461 ctgattcaat gagataaaag tgggtatcga cataacgctc gcggatgatg gcgctgacgg
     5521 ttttcgcaat caccgcctgg ggtagctggc ggcgcacctg ccagcgtggc gattccggca
     5581 gaaccaccag cagtgcaaca aacagcatca gcggtaatac gccacccaac accaggatcc
     5641 cgtgccagcc aatcaccggc accagctgcg cgctgacaat cccgccaaac gccgaaccca
     5701 gggtaaagcc gcaaaacatc agcgtcacca gcgcgccgcg gcggcgggca ggcagatatt
     5761 ccgaggtcat ggtaattgta tttggcatcg cgccacccag cccaaggccg gtgaggaaac
     5821 gcaggaatac cagcgtttgc agatccggtg agaaagccga ggcgaggctt aacgcgccaa
     5881 acaaaaagac gcacagctca atcacccgtt tgcggccaaa gcgatcggac aacggaccac
     5941 aaagcagtgc gcctgcggtt aaccccagca gacccgcgcc aaaaagcggg gcgagatcgc
     6001 ctgcggtgag ttgccagtga gtgcgaatat caggggcgat aaagccgata gccgcggtgt
     6061 caaaaccatc gagcattacc accagaaaac agcagatgat aacgcgccac tgcatcttgc
     6121 cgaccggagc agcatcgatc agtgcttgta aatcacgtcg ttgagtcata gtaaatgcct
     6181 cagtgcaggt aaggtagatg tttcatttgt tttatgtatg ttgcgatatt gtgattaaag
     6241 agatgaaatg tacaatggca tttcggacag agagataacc tggaggttat ggcaaatggc
     6301 aaactgggcg cagaaactaa agcttcatca cctgcaaatg ctggtggcgc tgggtgaaca
     6361 gggcaatctg acccatgtcg cgcggatgat gaatatcacc cagcctgcgc tgtcgaaatg
     6421 gttgtcacaa ctcgaagatg agatcggcct cacactcttt gagcggcaca gcaaaggact
     6481 tcgtcctact gaaggcggca aattgttatt gcaacatgcc caacggttaa tcaatgacat
     6541 ggagcgctcg caacatgaac tggcccgttt taaacaaggt ggactggtcg gcagtctgaa
     6601 aattggctgt tctcccgtcg ccactgactg cgtatcccag gcgattttgc atttgctcaa
     6661 ggagatgcca actctacacc tgaatatcga agagaaggtg atgaccccat tgctgcaaga
     6721 tctgttggca ggttctgtcg atgtggtggt tgggcgaatt ggcggtcgcg cgttgcagct
     6781 gccgctgaat tatcgcgtgc tctacaccga gccggtgtgt tttgtcgccc gtcctgagca
     6841 tccactggcg aaatacgcca ccctcagctg gaatgatctt gctaactggc ggtggatagt
     6901 ctggccgaca ggtaccccca tccgcatcag cattgataat gcgctggtag accacggcgt
     6961 gatgctgcca gaaaacacga ttgagtcggc gtcgatgaac gtcagtacca acctgttaca
     7021 aagcagcgat atgatctcta ttttgtcatt acgcctggcg caacgttatg ccagccaggg
     7081 acaactggtg attctcaacc tgccaaagat agaacagaaa ggcagcgtcg gtgtgttctg
     7141 gcgcaataac gaaacgcctt cttatgcttt aagtcagttt ctgcactttt tacagcagaa
     7201 ttagtgtgct gaactgggcg ctcgcccgag gttacgtccc taaaattcaa ctatgctgag
     7261 atttcttaat gtgatcggta gcacgtttta cgaattaatt gtatgatgaa tccatctcat
     7321 ctggggtgtt gattatgagt aagacactga acattatctg gcaatattta cgtgcttttg
     7381 tcctgattta tgcctgcctg tatgcaggca ttttcattgc ttccctgcta ccggtaacca
     7441 ttccgggcag catcatcggg atgctgatcc tgtttgtcct gctggccttg caaattcttc
     7501 cggcgaaatg ggtcaatccg gggtgctacg tactgattcg ctatatggcg ctattgtttg
     7561 tgccgattgg tgtaggcgtc atgcaatatt ttgatttgct ccgcgcacag tttggtccgg
     7621 tagtggtttc ctgtgcagtc agtacgctgg tggtttttct ggtggtgagc tggagttcac
     7681 aactggtgca cggtgaacgt aaagtcgtag gtcagaaagg atcagaagaa tgatggcgaa
     7741 tatctggtgg tcattaccgc tgacgttgat tgtctttttt gccgcccgca aactggcggc
     7801 acggtataag tttcccctgc ttaacccgtt gctggtagca atggtggtga tcattccttt
     7861 tttaatgctg accggcatct cttacgacag ctactttaaa ggtagcgaag tgctaaacga
     7921 tctgcttcaa ccggcggttg tcgcactggc ctatccttta tatgagcagc tgcaccagat
     7981 ccgcgcgcgc tggaaatcga tcatcaccat ctgttttatc ggcagcgtgg ttgcgatggt
     8041 gacggggact tctgtggcat tgttgatggg cgcttcaccg gaaatcgccg cgtcaatcct
     8101 gccgaaatca gtcaccacgc ctattgcaat ggcggttggc ggcagtattg gcggtattcc
     8161 ggcaatcagc gcagtttgcg tgattttcgt cggcatcctc ggcgctgtgt ttggccatac
     8221 attgcttaat gcgatgcgta ttcgtaccaa agctgcgcgc ggtctggcaa tgggcacagc
     8281 ctcgcacgcc ctcggtacgg cgcgctgcgc cgagctggat tatcaggaag gtgcatttag
     8341 ttcgctggcg ctggtgttat gcgggattat tacttcgctg atcgcaccgt tccttttccc
     8401 gattattctg gcagtaatgg gctaaaattt gcgatgcgtc gcgcattttt gatgtaagtt
     8461 tcacgcgttg cataattaat gagattcaga tcacatataa agccataacg ggttcgtaaa
     8521 ctgttatccc attacatgat tatgaggcaa cgccatgcat ccacgttttc aaaccgcttt
     8581 tgcccaactt gcggataact tgcaatctgc actggaacct attctggcag acaagtactt
     8641 ccccgctttg ttgaccgggg agcaagtctc atcgctgaag agcgcaacgg ggctggacga
     8701 agacgcgctg gcattcgcac tacttccgct ggcggcagcc tgtgcgcgta cgccattgtc
     8761 gaattttaat gttggcgcaa ttgcgcgcgg tgtgagcgga acctggtatt ttggtgccaa
     8821 tatggaattt attggtgcga caatgcagca aaccgttcat gccgaacaaa gcgcgatcag
     8881 ccacgcctgg ttgagtggtg aaaaagcgct tgtagccatc accgttaact atacgccttg
     8941 tggtcactgc cgtcagttta tgaatgaact gaacagcggt ctggatctgc gtattcatct
     9001 gccgggccgc gaggcacacg cgctgcgtga ctatctgccg gatgcctttg ggccgaaaga
     9061 tctggagatt aaaacgctgc tgatggacga gcaagatcac ggctatgcgc tgacgggtga
     9121 tgcgctttct caggcagcga ttgcggcggc aaaccgttcg cacatgcctt acagtaagtc
     9181 gccaagcggt gtcgcgctgg aatgtaaaga cggtcgtatt ttcagcggca gctacgctga
     9241 aaacgccgca ttcaacccga ctctgccacc gttgcagggg gcgttaattc tgttgaatct
     9301 caagggttat gattacccgg atatccagcg cgcggttctg gcagaaaaag ccgatgcgcc
     9361 gttgattcag tgggatgcca cctccgcaac actgaaagct ctcggctgtc acaatatcga
     9421 ccgcgtgctt ctcgcttaag cctggtgccg gatgcggcgt gaacgccttg tccggcttgc
     9481 cagccctctc ctggtgtcga aaatcccggc aaacagtttg ccgtttcttg cgcaaaacca
     9541 gcgggtaaag tagcctgatg gaaattttcc ttagatcgag tctcctgcat gttaaagcgc
     9601 gtgttcctca gcctgttagt cctgatcggc ttgctgctgt tgactgtgct cggcctcgat
     9661 cgctggatga gctggaaaac cgcgccttat atctacgacg aattgcagga tctcccctac
     9721 cgccaggtcg gtgtggtgct cggaacagca aaatattatc gtactggcgt aattaatcag
     9781 tattatcgct accgcattca aggagcgatt aatgcctata acagcggtaa ggtaaattat
     9841 ctattactga gcggcgataa cgcattgcaa agttataatg agccgatgac catgcgcaaa
     9901 gatttaatcg ctgctggtgt cgacccatca gatattgttc tcgattacgc aggctttcgt
     9961 acgctggatt ccatcgtgcg tacacgcaaa gttttcgata ctaatgattt cattattatc
    10021 acccaacgtt tccactgtga gcgggcatta tttattgcgc tgcatatggg gattcaggct
    10081 cagtgttatg ccgtaccgtc accgaaagat atgctgtcag tacgtattcg cgaatttgcc
    10141 gcccgtttcg gtgcgctggc tgacctttat atttttaaac gtgaaccgcg ttttttaggg
    10201 ccgctggtcc ctattccggc tatgcaccag gtgcctgaag atgcgcaggg gtatcccgcc
    10261 gtcacacccg aacagttact tgaattacaa aagaaacaag gaaagtagtt atggatgtac
    10321 agcagttttt tgtcgttgcc gtttttttcc ttatcccggt attttgtttc cgcgaagc
//
LOCUS       DAZGAP010000178        10210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGAP010000178 DAZGAP010000000
VERSION     DAZGAP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.179"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..222
                     /locus_tag="ACHLAM_004186"
                     /pseudo
     CDS             1..222
                     /locus_tag="ACHLAM_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(311..1447)
                     /gene="yibH"
                     /locus_tag="ACHLAM_004187"
     CDS             complement(311..1447)
                     /gene="yibH"
                     /locus_tag="ACHLAM_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7855637.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1450..1812)
                     /gene="yibI"
                     /locus_tag="ACHLAM_004188"
     CDS             complement(1450..1812)
                     /gene="yibI"
                     /locus_tag="ACHLAM_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7855638.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2349..4262
                     /gene="mtlA"
                     /locus_tag="ACHLAM_004189"
     CDS             2349..4262
                     /gene="mtlA"
                     /locus_tag="ACHLAM_004189"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO7855639.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4627..5775
                     /gene="mtlD"
                     /locus_tag="ACHLAM_004190"
     CDS             4627..5775
                     /gene="mtlD"
                     /locus_tag="ACHLAM_004190"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7855640.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5775..6362
                     /gene="mtlR"
                     /locus_tag="ACHLAM_004191"
     CDS             5775..6362
                     /gene="mtlR"
                     /locus_tag="ACHLAM_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO7855641.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6373..6582)
                     /gene="yibT"
                     /locus_tag="ACHLAM_004192"
     CDS             complement(6373..6582)
                     /gene="yibT"
                     /locus_tag="ACHLAM_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO7855642.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            6866..7228
                     /gene="yibL"
                     /locus_tag="ACHLAM_004193"
     CDS             6866..7228
                     /gene="yibL"
                     /locus_tag="ACHLAM_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO7855643.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            7787..8470
                     /locus_tag="ACHLAM_004194"
     CDS             7787..8470
                     /locus_tag="ACHLAM_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO7855644.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            8514..>10210
                     /locus_tag="ACHLAM_004195"
     CDS             8514..>10210
                     /locus_tag="ACHLAM_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO7855645.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAANAIAMGAESKAAENATAVGTNAEANGLNSI
                     ALGSGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIAL
                     GQGSYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGD
                     ISTTSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTG
                     LDNNTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATN
                     TANITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNVKDGDLTAGST
                     "
BASE COUNT         2725 a         2471 c         2524 g         2490 t
ORIGIN      
        1 aaatattttt aaatgtgact gtcatccttc agataataaa gccatcatcg caatagatgg
       61 aaaagcactc ctgctctttt ttgattagag ccgtggcaga ggaacaactc atatcatcat
      121 tgcgatctcg acaatgtata gtctgatcct tcggcagatc aagacggata aaaaatccaa
      181 tgagttcaca ctcatcccga acttcttaac ataatggata ttaacgaaaa atcatcacag
      241 ccgatgaaat aatatgcaaa aataattagt ccttacaagg agcaaagttc cataatctcc
      301 cccctccccc tcaatgatcc aaataaagat aatgcatcca actggtcatt ctcagcagca
      361 ctttccgcat caccgaaaca tggctgaaat ggtcggagta aactgccact tgggcgtaga
      421 tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt cccagcacac
      481 cgtccgtgcc aggcacgacc gttaatgatt gcaatacccc ctgcgcctga taagaaccgc
      541 ctggcacgac aggtaaaata ctagtcagtt tgccgtgaaa cacctgccca ggtagcgcgt
      601 taaacaccac ttccgcatca tcgccgggtt tcagacgtaa cagcgagttt tgccgaaatt
      661 gggcgacaat ttgccgtttt tgctcgggga tgaaaaccat caccggacgc aacggcaagg
      721 cagctgcgta tgtacctggg cggatcagta cctgagtaac gtagccattg cttggtgcgc
      781 gaatgacagt ctgctcaagg ttatattttg cttcagtaag ttgcgctctt aagctcacaa
      841 tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc gcctgctccg
      901 ccaccgagcc tttcaccagc gcgtcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt
      961 cacgttccga gaacggattc accgccgcct ggctgccttt caggtaacgt tgataatttt
     1021 taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct
     1081 gcgcgcgcag cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa
     1141 ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt tgaataagct
     1201 gattattttt gtcggtgact tcagtaacaa ttcccgtcac ctgtggcgtg atggggatcg
     1261 ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca
     1321 aaccactcac cagaaacacg cctcccagcg ccgcggtcgc cagcgtccac tgattcaccg
     1381 gaatgcggaa gattttaaag accgcccacg ccagcgccac gtaagttaaa acaatcaata
     1441 gatccatatt tagatctccg ggaacgtgga atagtcagtc tttttctcgg cggcgagttg
     1501 atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccgggtcag tcgctttctc
     1561 ctgcgacgca acatgtgact gcatacccca gccacgctcc ggttgataga gcgtcgccca
     1621 gatccacagg aacggccaga taacatgcag agtaaacagg ctcacccagc ccgccgtatg
     1681 aatagcgtcg gcatggggat gattgcgctt tttggcaatc agatatggga tgtcatgtat
     1741 cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca acgcgaaata
     1801 gtttagaaac atatctgcct cggattcacg tttatcagtg ttgttttttg gctggcagcc
     1861 agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc gccataacac
     1921 caaagaataa tttttagagg tgatgagttg cttagttaca taacgatttt atgacgaagg
     1981 cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg tgattcagat
     2041 cacaaagatc caacaaacca ccaaaacaaa aatgtgacat tagtcacatt taaatgccat
     2101 ttttagcgaa aatcgccgtc ttgttgcttt tttacacaag cattttgtga tgagcatcac
     2161 gtcaattacc tctctacccc ctatatttat gtgacccata tcacacaaaa ggccgtcgac
     2221 tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg gctacctctg
     2281 ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg
     2341 gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt ttcctcagca
     2401 acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta
     2461 ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg atgatcactt
     2521 atctcctgcc gctgctgatc ggttataccg gtggtaagct ggtaggcggc gaacgtggcg
     2581 gcgtagtcgg tgccatcacc accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc
     2641 tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc gaccgctggg
     2701 tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc gcaggcatca
     2761 tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt gaagccctgt
     2821 ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg ccgctggcgt
     2881 ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac cacggtatct
     2941 tctcgccgct gggcattcag cagtcccatg aattgggtaa atccatcttc ttcctgattg
     3001 aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt ggtcgtggca
     3061 gtgctaaaca atctgcgggc ggtgcggcaa tcatccactt cctgggtggt atccacgaaa
     3121 tttacttccc gtatgtgttg atgaatccgc gtctgatcct ggccgttatc ctcggcggta
     3181 tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg gcatctccgg
     3241 gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttatttcgct aacatcgcag
     3301 gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc cattctgctg aaaaccagca
     3361 aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac atgaaagctg
     3421 agtctaaagg tgcgtctccg ctgtctgcgg gcgatgtgac taacgacctg agccacgtac
     3481 gtaaaatcat cgttgcctgt gacgccggta tgggttccag tgcgatgggc gcaggtgtgc
     3541 tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc ggtcactaac agcgcgatca
     3601 acaacctgcc gccagatgtg gacctggtca tcactcaccg tgacctgacc gaacgcgcta
     3661 tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac agcggcttgt
     3721 acaccagcct gaccgaacgt ctggttgctg cccagcgcca tactgaaaac gaagtaaaag
     3781 tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc aagctaggcg
     3841 cggagaacat cttcctcggt cgcaaagcgg ccaccaaaga agaagcgatt cgttttgctg
     3901 gcgagcagct ggtgaaaggc ggttacgttg agccagaata cgttcaggcg atgctggatc
     3961 gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac ggtacggttg
     4021 aagcaaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg gaaggcgtgc
     4081 gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgct gcccgtaaca
     4141 acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgaa tccgtcatcg
     4201 agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca ggtcgtaagt
     4261 aatacaatct caccctctcc acatggagaa ggtggggctt attgcctgat gcgctacgct
     4321 tatcaggcct acaaaacaaa gcacagtttg ttgaatttgc acgttcttgt aggccggata
     4381 aggcgtttac gccgcatccg gcgctgcccc tctccacatg gagaagatgg gctaattgcc
     4441 tgatgcgcta cgcttatcag gcctacaaaa acaaagcaca atttgttgaa tttgcacgtt
     4501 cttgtaggcc ggataaggcg tttacgccgc atccggcgcg gcccctctcc acatggagag
     4561 ggtttgggtg agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt
     4621 aatactatga aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa
     4681 ctgctggcag acgcgggtat ccaactgacg tttgccgatg tcaatcaggt ggtacttgat
     4741 gccctgaatg cccgtcatag ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat
     4801 accgtttccg gcgtcaatgc cgtcagcagc attggtgatg atgtcgttga tctgattgct
     4861 caggttgatt tagtcactac cgccgttggc ccggttgtgc tggaacgtat tgctccggca
     4921 atcgccaaag ggctggtgaa acgtaaagaa caaggtaatg aatccccgct gaacatcatc
     4981 gcttgtgaaa acatggtccg cggcaccaca cagctgaaag gccatgtgat gaacgccctg
     5041 ccagaagatg ccaaagcgtg ggtagaagaa cacgttggct ttgtcgattc cgccgttgac
     5101 cgcatcgtgc cgccttcggc ttctgcaacc aacgatccgc tggaagtgac ggtagaaaca
     5161 ttcagcgaat ggattgtcga taaaacgcag ttcaaaggcg cgctgccaaa catccctggc
     5221 atggaattaa ccgacaatct gatggcattt gtcgaacgta aactcttcac cctgaataca
     5281 ggtcatgcta taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgatgcg
     5341 attctcgacg agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggtgcagta
     5401 ttgatcaagc gctacggctt tgacgcagac aaacatgcgg cgtacatccg gaaaatcctc
     5461 ggtcgtttcg agaacccgta cctgaaagat gatgtagagc gcgtaggccg tcagccgctg
     5521 cgtaaactga gtgctggcga ccgtctaatc aagccactgc tcggaacgct ggaatatggt
     5581 ctgccacata aaaacctgat tgaaggtatt gccgctgcaa tgcacttccg cagtgaagat
     5641 gatccgcagg ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg
     5701 gcacagattt ccggtcttga tgccaacagc gaggttgtat ccgaggcggt aaccgcttat
     5761 aaagcaatgc aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt
     5821 cagatatgca ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc
     5881 tgaatgctgg caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg
     5941 cggtaaatct tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg
     6001 tagaaccgtt actcgacggc gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca
     6061 tttacgggtt gggcgtcatt aaccgccagg aatacgaaga tgcggaactg ctgatggcat
     6121 tgcgtgaaga gctaaatcac gacggcaacg agtacgcgtt taccgacgac gaaatccttg
     6181 gaccctttgg tgaactgcat tgcgtggcgg cgttaccacc gccgccacag tttgaacctg
     6241 cagactccag tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa
     6301 tggtcctttc actgacagag ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt
     6361 aagtactttc gcttacttct cctctacctg tttcggacga taaagctgac gataatactc
     6421 cagacgttgt agatataacg gcacgctttc atcaggtatt ccggacggaa tcgcgttacg
     6481 gggaggtagt tttttcagat actcccggaa cgcctggctc gatgccatga aatctacggc
     6541 tttatcgata agaagcggaa cgttttcacc taatttgccc atgatgatat ctccgtatta
     6601 ccccgcgccg ggaatgcgcg gccgccaatt ttagtttaga tcccggtata aatgacatta
     6661 aggaaaacgt gctcaatcct caataatcac ctgcttctgt tgactatctt ttttctttaa
     6721 atttcttaat taatcaataa attgcgtagt ttttaagcat gttaatgttt acgttataga
     6781 actattgcaa tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta ggggctataa
     6841 atttatcttt atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct
     6901 cagcgatcgt ctggacgcca tccgccacca gcaggccgat ctgtcgctgg ttgaagccgc
     6961 agacaaatat gccgaactgg aaaaagagaa agccactctg gaagcagaaa ttgctcgcct
     7021 gcgcgaagtt cacagccaga aactcagtaa ggaagcacaa aagctgatga agatgccatt
     7081 ccagcgcgcg attaccaaaa aagagcaggc tgatatgggc aagctgaaga aaagtgttcg
     7141 cggactggtc gttgtgcacc caatgaccgc gctgggccgc gaaatgggcc tgcaagagat
     7201 gacagggttc tcaaagactg cgttttaagt tcctttctcg tcgctgtaat cacagcctct
     7261 caccatttac tcgctcctgc tggcacatgc aggagcctgt ttcagagatc gaatatttat
     7321 aacgttctta acattatata ttcaatctcc tctattcttt ttagcgcaca tgccaggcta
     7381 aattctgaaa atcaggtaca agttaaagaa cgatgtatca atattaacaa tgaattgccg
     7441 catatcaatg cttgaaacat aattaattat ttaaattttt ctatcagcat cgcaaacttc
     7501 gcaataaaac atcactcaac tgaatattta cctttccctg ccctgggcct ttatccggtg
     7561 aaacatttga cattgattga caccgatcat taaaatccgc gcgattaata ttattggcta
     7621 caaaccgaaa ttgtttgaca cttaataaca tattcgcaca accgattaat acaaaaattc
     7681 tctaacaaat ttatttgtcg gatatgctcc actctaatca ttcataaaaa aaatgattgt
     7741 gccgtaatta tcaactgtat cgggattatg aaaaggaatt catcctatga acctaaagaa
     7801 aacactgtta agcgtgttaa taatagtgcc tctctgctta ttggcagggt gtgactatat
     7861 tgaaaaagcg agtaaggtcg acgacctcgt tacacagcaa gagttgcaaa aaagcaaaat
     7921 tgaggcgctt gagaaacaac aggaactcga taagcgaaag atagaacatt ttgaaaaaca
     7981 acaaactact atcataaata gtaccaagac gctcgctggc gtggtgaagg cggttaaaga
     8041 caaacaggac gaatttgtct ttacagaatt taacccggca caaacccaat acttcatttt
     8101 aaataacggc tctgttggtt tggcaggccg ggtgctggct attgatgcag ttgaaaatgg
     8161 cagcgttatt cgtatttcgc tggttaattt attaagcgtt cccgtatcaa atattggctt
     8221 tcatgcgaca tggggcaatg aaaggcccac cgatgccaaa gctctggcaa agtggcaaca
     8281 attacttttc aacaccacac tgaattccac tctgcaatta atgcctggtc aatggcaaga
     8341 cattaatctg acattaaaag gcgtttcgcc taataacctg aagtatctga agttgtccat
     8401 caatatggcc aatctccagt tcgacactgt tcaatctgct gaaactcggc agcgaaaaaa
     8461 caaaaaataa atgcagcttc tgcgcttata tcaaggaata gagagcatca ataatgaaca
     8521 aaatttttaa agttatctgg aatccagcga cgggcagtta cagcgttgcc agcgaaacgg
     8581 cgaagagccg tggtaaaaaa agcgggcgca gtaagctgtt aatttctgta ctagttgcga
     8641 gtgggttgtt gtcgtcgttt ggagcgttag ctaataacta cgacgggcag ccaactgatt
     8701 atggcgatgg ctcagcaggt gacggctggg ttgctatcgg taaaggggca aaagcaaata
     8761 cctttatgaa cactagtggc gcgagtacag ctttaggata tgacgcgata gccgaaggtg
     8821 agtacagttc tgccatcggg tcaaaaaccc ttgcaactgg tggagcatcc atggcgttcg
     8881 gggttagtgc aagagcaatg ggtgacagaa gtgtcgcgct gggtgcatcg tcagtagcaa
     8941 atggcgatcg ttcgatggct tttggtcgtt acgcaaagac gaatggttat acatctcttg
     9001 ctattgggga ctcctccctt gccgatggtg aaaaaactat tgcgttagga aatacggcta
     9061 aagcttacga aattatgagc atcgccctcg gtgatcatgc caatgcgtca aaagaatatt
     9121 caatggcgct gggtgcaagt agtgccgcat ctgcggctaa cgcgattgca gtaggccgca
     9181 acagtgctgc agctggcgta gacagcctcg cattcggtag actatccgcc gccaacgccg
     9241 ccaacgccgc aaacgcgatt gctatgggtg cagagagcaa agctgctgaa aatgcgacgg
     9301 cagttggtac taacgcggag gcaaatggac ttaatagtat cgcgttgggc tccggcagta
     9361 ttgcagatgt cgacaacact attgctctgg gcaatcaaag tcaggcagta gcggctggcg
     9421 caattgccat cggtcaaggg aataaagctg acggcgcaaa tgctatcgcg ctgggtaatg
     9481 gtagtattac aggtggtgta aatgctattg ctcttggaca aggcagttat gccggtttag
     9541 aaaatggcac tgcaattggt gctcaagcca gtgctcaggg gaaaaattca gttgctctgg
     9601 gtgctggttc tgtagcgact gacgcggata ctgtttctgt gggtaacaca acagctcagc
     9661 gacaaattgt caatatggca gcaggtgata tcagcactac cagtactgat gccatcaatg
     9721 gatcacagct ttatgctatc agtaagtcag tagcggacaa tcttggtggg ggggctaccg
     9781 tcaatgcgca aggcgtcgtt acttccccaa attacaggct gaaaagtggt attttcggca
     9841 ctgttggcga cgccttaacg ggcctggaca ataatacgtt acaatgggac tcccttaaaa
     9901 aggcatatag tgcggcacat ggtacagata ctaccagtac catcaccaac gttaaagacg
     9961 gcgctatttc tgataccagt aaggatgcgg ttaacggttc tcagctgaaa accaccaacg
    10021 ataacgtagc gaccaatact gccaatatca ccaccaacac taacagtatc aataccctga
    10081 cggattccgt tggcgacctt aaagacgatg ccctgctgtg gaatggcacc gcgttcagcg
    10141 ccgcgcacgg caccgaagcc accagcaaaa tcaccaacgt caaagacggt gacctgacgg
    10201 ctggcagcac
//
LOCUS       DAZGAP010000179        10166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGAP010000179 DAZGAP010000000
VERSION     DAZGAP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.180"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..604
                     /locus_tag="ACHLAM_004196"
     CDS             38..604
                     /locus_tag="ACHLAM_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7855646.1"
                     /translation="MFKGQKTLAALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            655..3114
                     /locus_tag="ACHLAM_004197"
     CDS             655..3114
                     /locus_tag="ACHLAM_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7855647.1"
                     /translation="MDTVNIYRLSFISCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKGKGVIVPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSLHLPEHMEDTANRLILPCH"
     gene            3129..3860
                     /locus_tag="ACHLAM_004198"
     CDS             3129..3860
                     /locus_tag="ACHLAM_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7855648.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            3857..4918
                     /locus_tag="ACHLAM_004199"
     CDS             3857..4918
                     /locus_tag="ACHLAM_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7855649.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPTNIHMSRTFLA
                     RMRLYVKIREIPPHDYQSSLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            5071..6117
                     /locus_tag="ACHLAM_004200"
     CDS             5071..6117
                     /locus_tag="ACHLAM_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO7855650.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATNPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6114..6905)
                     /gene="nei"
                     /locus_tag="ACHLAM_004201"
     CDS             complement(6114..6905)
                     /gene="nei"
                     /locus_tag="ACHLAM_004201"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO7855651.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6941..7675)
                     /gene="pxpA"
                     /locus_tag="ACHLAM_004202"
     CDS             complement(6941..7675)
                     /gene="pxpA"
                     /locus_tag="ACHLAM_004202"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO7855652.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7665..8597)
                     /gene="pxpC"
                     /locus_tag="ACHLAM_004203"
     CDS             complement(7665..8597)
                     /gene="pxpC"
                     /locus_tag="ACHLAM_004203"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO7855653.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8591..9247)
                     /gene="pxpB"
                     /locus_tag="ACHLAM_004204"
     CDS             complement(8591..9247)
                     /gene="pxpB"
                     /locus_tag="ACHLAM_004204"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO7855654.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9270..10013)
                     /gene="ybgI"
                     /locus_tag="ACHLAM_004205"
     CDS             complement(9270..10013)
                     /gene="ybgI"
                     /locus_tag="ACHLAM_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO7855655.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
BASE COUNT         2500 a         2752 c         2447 g         2467 t
ORIGIN      
        1 tcgcaacact ctatctcatt atttaaagga ctcagctatg tttaaaggac aaaaaacatt
       61 ggccgcactg gccatatctc tgctattcac agcacctgtt tatgcggctg atgaaggatc
      121 cggtgaaatt cactttaaag gtgaagttat tgaagcaccg tgtgaaatac atcaggatga
      181 tattgataaa gaggttgaac tcggtcaggt gaccaccagc cacattaatc agtcgcatca
      241 cagcgatgcc gttgctgtcg acctgcgctt agtcaactgc gatctggaaa actccaacaa
      301 cggttccggc ggcaagattt ctaaagttgc agtcaccttt gacagctcag cgaaaaccac
      361 cggcgcagat ccgattctca acaacaccag cacgggtgaa gcgaccggtg tcggcgtgcg
      421 tttaatgaat aaagatcaaa gcaatatcgt tttaggcacc gctactccag atatcgacct
      481 ggcaccaacc tccagcgaac aaacgttaaa tttctttgcg tggatggaac aaattgatca
      541 ggctacgccc gtaacaccag gcgcagttac ggcgaatgcg acctacgtac tggattataa
      601 ataagtatca ctttggcggc gtcatcgtcg cctttcttca aatatttatc aggtatggac
      661 accgtgaata tttatcgact ctcttttata tcctgtctgg tcatggcgat gccttgcgca
      721 ttggcggtcg aattcaacct gaatgtcctc gataaatcga tgcgagatcg cattgatatt
      781 tcattattaa agggaaaagg ggtcattgtt cccggtgaat attttgttag cgttgcagta
      841 aataacaacc aaatcagtaa cggacaaaaa attgactgga aaaagaacgg tgaccaaacc
      901 attccgtgta ttaatgattt actggtagat aaatttggtt taaaacctga agttcgtcag
      961 tcgttaccac ggttaaacca gtgcgtcgat tttagctccc ggcctgaaat tctcttcatt
     1021 tttgatcagg ctagccagca actgaatatc actattccac aagcctggct ggcgtggcac
     1081 tcagataact ggactccccc ctcaacatgg aaagaaggtg tcgccggcgt cctgatggac
     1141 tacaacctgt ttgcaagcag ctaccgccca caggatggta gcaacagcac taacctgaac
     1201 gcctatggaa cagccggaat taacgcggga gcatggcgtt tacgcagtga ttaccaattg
     1261 aatcagaccg atagcgatga taaccatgaa cagtcaggcg aaatatcacg cacctatctt
     1321 tttcgtccat taccgcaatt aggttcaaaa ttaacccttg gtgaaacgga tttcagttcc
     1381 aatattttcg acggcttttc ttataccggc gcggcactgg caagtgacga tcggatgtta
     1441 ccgtgggagc tacgcggcta cgccccacaa atcagcggca ttgcacaaac caacgccacc
     1501 gtcaccgtga gtcactcagg gcgagttatt taccagaaga aagtcccacc aggtccgttt
     1561 atcattgatg accttaatca gtccgtccag ggtacgttag atgtcaaagt gacggaagaa
     1621 gatggtcggg cgaacaattt ccaggtttcg gcagcatcga cgcctttcct tacgcgccag
     1681 gggcaggttc gctataaact tgctgccggt cagccgcgaa catctatgtc tcatcaaact
     1741 gaaaatgaaa ccttttttag caatgaagtt tcctggggga tgctgtcaaa tacctcactg
     1801 tacggcggcc tgctgatttc gggtgacgac taccattctg ccgcaatggg tatcgggcaa
     1861 aatatgctgt ggcttggtgc attgtcgttt gatgtcacgt gggccagtag tcagtttgat
     1921 actcagcagg acgagcgggg cttaagttac cgctttaatt acagcaaaca agtggatgcc
     1981 accgacagca cgatttcgct cgccgcttat cgtttctccg atcgtcattt tcacagctac
     2041 gccaactatc tggatcacaa atacaacaac agcgatgcgc aggacgaaaa acagacgatc
     2101 agcttatccg tgggccaacc gattacccca ctgaacctca atctgtacgc caacctgctg
     2161 catcaaacct ggtggaatgc agacgcctcc acgaccgcca acatcacggc gggttttaat
     2221 gttgatattg gtaactggag agatatctcg atctcgacgt cattcaatac aacccattac
     2281 gaagataaag atcgcgacaa tcagatttac ctgtcgattt cgctcccctt cggtaacggg
     2341 gggcgggtcg gttatgacat gcaaaacagt agtcacagca ccacgcaccg catgtcgtgg
     2401 aatgatacgt tagatgaacg taatagctgg ggcatgtcta ccggactgca atccgaccgt
     2461 ccggataatg gagcccaggt gagcggtaac tatcagcacc tgagttcagc gggtgagtgg
     2521 gatatttctg gtacctatgc cgccaatgat tacagttccg tcagcagcag ctggagcggt
     2581 tctttcacgg caactcaata tggtgcggcg ttccatcgtc gcagctccac caatgaacca
     2641 cgtctgatgg tcagtaccga tggcgtggca gatattccgg ttcagggcaa tctcgactac
     2701 accaaccatt ttggcattgc ggtggtgccg ttgatttcca gttatcagcc ttccaccgtg
     2761 gcggtgaaca tgaatgactt acccgacggc gtaacagtgg cagaaaacgt tatcaaagaa
     2821 acgtggattg aaggcgcgat aggttacaaa tcgctggctt cccgttccgg taaagacgtt
     2881 aacgtcatca ttcgcaacgc cagcggtcag ttccctcccc tcggtgcgga tatccgccag
     2941 gataacagcg gcattagcgt ggggatggtt ggcgaggaag gacatgcctg gttaagcgga
     3001 gtcgctgaaa atcaacaatt taccgtgatc tggggtgata gccagcgttg ctcgctccat
     3061 cttcctgaac atatggaaga caccgcaaat cgcctgattt taccttgtca ttaattaagg
     3121 aaatgaaaat gacatttatg aaaggactac cccttttgtt gttggttgcc agtctgtgca
     3181 gccacgctgc actacaaccc gatcgcactc gtattgtgtt taacgctaat gataaagcta
     3241 ccagtctgcg cgtggataat cgtagcgata aactccccta cctcgcttat tcctggctcg
     3301 aaaatgaaaa aggggagaaa agcgatgatc ttctggttgc cctgccaccg atacaacgtc
     3361 tggaaccgaa agcaacaacg caggtacgga tagtaaagca agcctcgacc acaaaattac
     3421 cgggcgatcg cgaaacgcta tttttctaca atatgcgtga aattcctccc tccccagaaa
     3481 aaaacagcgg ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag gtattctggc
     3541 gaccggctgc attacgcaag aaagcaggag aaaaggtcga actgcagtta caggtcagcc
     3601 agcagggcaa ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc attgcctatc
     3661 tggggagaaa tgaaaaaggc gttcttcccg gcttcaaaac cgtgatggtt gcgcctttta
     3721 gtacagtaat tacaaacacc ggaagttata gcggcagtca gttttatctc ggttatatgg
     3781 atgattacgg cgcgttacgc atgacgacgc tcaactgcag cggacaatgc cgtttacaag
     3841 cagtggaggc gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct gtctggtatt
     3901 tctgccattg aagtctgcgc tagcgctgaa ctgctatttt ggtacttccg gcggaacggt
     3961 agaaaaatcg gaagcgattc aaccctttgc tgtgccaggt aatgccaaac ccggcgataa
     4021 gatctgggaa tctgacgata ttaaaattcc cgtctattgt gacaacaata ccaacggcaa
     4081 ctttgaaagc gaacatgtct acgcatgggt aaacccctac ccgggggtac aggatcgtta
     4141 ttatcaactc ggcgtgacat acaatggtgt cgattacgat gccagtctgg ggaaaagccg
     4201 catcgacacc aaccagtgta tcgacagtaa gaacattaac atttataccc ctgagcagat
     4261 tatcgcgatg ggctggcaga acaaaatatg ctcgggtgac cccactaata ttcacatgtc
     4321 gcgaaccttt cttgctcgta tgcggttata cgttaagatt cgcgaaatac cgccgcatga
     4381 ttatcaaagt tcgcttagcg actacatcgt cgtgcaattt gatggtgccg gtagcgttaa
     4441 cgaagaccct accgcccaaa acctgaaata ccatattact ggtctggaaa acattcgtgt
     4501 actggattgc agcgtcaatt tttctatttc accagaaaca caggtgattg attttggtaa
     4561 atttaacctg ctggatattc gccgccacac aatgtcgaaa acattcagta ttaagacgac
     4621 aaaatcacaa aatgaccagt gcaccgacgg atttaaggtc agctcttcgt tttataccga
     4681 agaaacattg attgaagagg ataaagcact gctgattggt aatggactga agctacgttt
     4741 gctggatgaa aattcttcac cctatacttt caacaaatac agcgaatatg ctgatttcac
     4801 cagcgatatg ctgatctacg aaaaaaccta tacggctgaa ctttcgtcca ttcccggcac
     4861 ccccatcgaa gcgggtccct tcgatacagt ggtgcttttt aagattaact ataactaatt
     4921 tatcatgatg ccctttgcag ttagtaaagg gcctctcttt cagaaaattg ctgattttcc
     4981 ccttttcctt gattgctgtt attttttcct ggcccggctt tctttatgct gtatcgatac
     5041 accaaagaaa aaataatgag ggaacgaagg atgccagttt tgcagtgggg aatgttatgt
     5101 gtgctctcac ttctcctttc aattggtttc ctcgcggttc atctcccggc ggcgctatta
     5161 cttgggccga tgatcgccgg gatcatcttt agtatgcgcg gaattactct gcaacttccc
     5221 cgctccgctt ttctcgccgc ccaggccatt cttggctgca tgattgcgca aaacctgacc
     5281 ggttcgatcc tgaccacgct agcggtgaac tggccgattg tgttagctat tttactggtg
     5341 acgttacttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga
     5401 aataccggtg cctggggttc ttctcctggc ggcgcggcgg caatggtcgc tatggcgcaa
     5461 gattacggcg cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc
     5521 gcaggggccg ctgttctggt cacccgcatg atgctgggcg ataacgctga agcggttaac
     5581 cagcagatcg tctggtttcc gccagtgagc attaatctcc tgcttacgat tttgctcgcg
     5641 gtcgttgccg gtacagtggg atgcttgcta cgtcttcctt ccggcacgat gctcatcccg
     5701 atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg
     5761 ctgcttgcga tggcgtatat ggcaattggt tggcggattg gtcttggttt cgataaacaa
     5821 atcttactgc gggcattgcg cccgctaccg caaatcctgc tgtcgatttt tgctctgctg
     5881 gctatttgtg cgggtatggc gtgggggctg acccggttta tgcatattga ttttatgacc
     5941 gcctacctcg ccaccaaccc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc
     6001 aatgccgata tggcgctcat catggcgatg caaaccctgc gcctgttcag tattttgctg
     6061 acgggacctg ccattgcgcg gtttatttca acctatgcgc cgaagcgttc tgcctaatgc
     6121 tggcagccag ggcaccagta aaacgggcga gatgacagcg tggttttctc aatgatgccg
     6181 ccacagcgtt cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca
     6241 tgatgcttat tctcatccac ctgcccccgc gtagcgtagg aaaatcgagg aatatccagt
     6301 aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca
     6361 gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac
     6421 gcctgatcga gcagtaatcc agcaaactga cggttacgaa agcgcggcga caataaccgt
     6481 tcctttacca cctccggcgt cagattcgga tccagcacat cggggccgac tcgttgtaaa
     6541 aacggatgcg tgatcagttg ttccggcgtc aacatctcaa tatccgaggc gctataaagc
     6601 agaatagttt tgtcaaccgt ttgcagtttt acccgcaata ctcgcatagt ctgcggctct
     6661 tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaag
     6721 tcgttggaaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga
     6781 ccgataagtt gtgattgata aggttttaac tgcgggaagg caaaccagac atcagttagt
     6841 ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca
     6901 ggcatggtga tatccttttt tcattcttta ttaaacgggg ttatgctgcg acaacaatcc
     6961 ccttttcggc aaatgtagag cgtagtcggc gggcgaatgc cagtgcgtgc tcgccgtcgc
     7021 catgcaggca gaccgtttgc gccgtgaccg ttgcccattc gccggtgatg cttttgactc
     7081 tgccgtgttg caccatctcc agcgtttgcg ccagcgcctg ttcttcgttt tcaatcaacg
     7141 cgcctggctg gcttcgcggc accagcgaac cgtctgcctg ataaccgcga tcggcaaaca
     7201 cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca
     7261 gcccgacgag aatcaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca
     7321 gttgtgcctc tttcgctgcc tggttgtata acatgccgtg aggtttgaca tgacgcataa
     7381 caccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt
     7441 aaacggtttc tggcggcagc tgcatgacgc tgcgcccaaa attttccctg tcgggaaaac
     7501 tcgggtgagc gccaatcgca acaccatttt ttattgcttc acgcacgcaa gcctgcatgg
     7561 tttgcgcatc gcctgcatga aatccacagg caatattggc agaggaaacc agcgttaata
     7621 gctctgcgtc gctggcatag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc
     7681 agccgccacg ctaattgttc gaaataacgt tgctgatctt gccgtgcttt cagtgcttct
     7741 tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac
     7801 atatcagcct caatgataca ggcaatacgc ggataaccac cggtggtctg tgcgtcgttc
     7861 atcaacacaa tcggctgccc gttatgcggc acctgcacca cgcccggtaa caaaccatga
     7921 gataacagtt cgcgatcggt ggtacgtttt aaaatttgcc cctgtaagcg atagcccatt
     7981 cggttacttt gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga
     8041 tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt
     8101 ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca
     8161 tccttcagta aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac
     8221 cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc
     8281 tgcgggcgtt taagcgttaa acgctgacct gctttcatcg gcaagcgcca gccggtccag
     8341 acggcgttat catccagccg cgcttcgcaa ccggcccccg tcagagcaaa ccacccatca
     8401 gtttcgaact caaccgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca
     8461 ttacccacca gtaggttagc aatgcgcagc gcaggcatat ccagtgcgcc gcagtggctg
     8521 atacccgact ggcgaaaacc gtgacgaccg ccatcctgca cagtggtata catacccgca
     8581 cgaataatct tcagcatact ccctccttct gcggtacaaa gcgcacgctg tctcccggac
     8641 gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca
     8701 actgccagcc acccggcgtt gccaacggat aaataccagt ctgcggcccg ccgatcccga
     8761 cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg
     8821 gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca
     8881 cggaggagtg caattcaaca acctgttttt cgctcaatcc acaatgcgcc gcgaccaccg
     8941 ccaaatccgg tccggctgcg ccaccgtaaa ccaccggaat ttcaataaag cgagactccg
     9001 gctccagcgc ctcactctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca
     9061 gcgactcagg attacgcaaa atcactgtga tattgttcat gccaggaatg gcttcaacca
     9121 cattcggcat atccaccaga cgctgtgcca gtcgccagat ccgtttctgg ctagccagcg
     9181 tcaccggcgg ttccagctcc agcactaccg ccgtttcacc tatcagataa caacgcgctc
     9241 gttgcacttt cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc
     9301 acatcaagat cggtattttc attcagccac tcgctcaatg cgcgaatacc accgcgttca
     9361 gtggcatggt gacccgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc
     9421 tgttctgaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata
     9481 aaactctgcc cgccgcccgt gcaccaggcg acgcgctgaa ccacctcagg tccggtatcg
     9541 ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaagccag ctccagtccc
     9601 ggcacaggca tggtcagttc gccccacggc accaacggct caatttcgcc catgaccgtg
     9661 atccccagta acgccgccag ttgtgcgtta ttacccagct ccggatgcgc gtccaggggc
     9721 agatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg gttacgcttc
     9781 attccgcgaa tgaccggaga ttcacctttc cagaagtagc catgatgcac aatgaccgca
     9841 tccgcgccca gacgcaccgc ttcatcgagc aaagcctggc tggcggtgac accggtaaca
     9901 attttttgca ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc
     9961 gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg
    10021 ccttccgtat tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag
    10081 ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc
    10141 atcacgcttc agctaatgta tattta
//
LOCUS       DAZGAP010000180         9962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGAP010000180 DAZGAP010000000
VERSION     DAZGAP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.181"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..1047
                     /gene="glaH"
                     /locus_tag="ACHLAM_004206"
     CDS             70..1047
                     /gene="glaH"
                     /locus_tag="ACHLAM_004206"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO7855656.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            1067..2335
                     /gene="lhgO"
                     /locus_tag="ACHLAM_004207"
     CDS             1067..2335
                     /gene="lhgO"
                     /locus_tag="ACHLAM_004207"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO7855657.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALQEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2358..3806
                     /gene="gabD"
                     /locus_tag="ACHLAM_004208"
     CDS             2358..3806
                     /gene="gabD"
                     /locus_tag="ACHLAM_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7855658.1"
                     /translation="MKPNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3820..5100
                     /gene="gabT"
                     /locus_tag="ACHLAM_004209"
     CDS             3820..5100
                     /gene="gabT"
                     /locus_tag="ACHLAM_004209"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO7855659.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYI
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5338..6738
                     /gene="gabP"
                     /locus_tag="ACHLAM_004210"
     CDS             5338..6738
                     /gene="gabP"
                     /locus_tag="ACHLAM_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO7855660.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6759..7421
                     /gene="csiR"
                     /locus_tag="ACHLAM_004211"
     CDS             6759..7421
                     /gene="csiR"
                     /locus_tag="ACHLAM_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO7855661.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7422..7871)
                     /gene="kbp"
                     /locus_tag="ACHLAM_004212"
     CDS             complement(7422..7871)
                     /gene="kbp"
                     /locus_tag="ACHLAM_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO7855662.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7955..8113)
                     /gene="yqaE"
                     /locus_tag="ACHLAM_004213"
     CDS             complement(7955..8113)
                     /gene="yqaE"
                     /locus_tag="ACHLAM_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO7855663.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8296..8595
                     /gene="ygaV"
                     /locus_tag="ACHLAM_004214"
     CDS             8296..8595
                     /gene="ygaV"
                     /locus_tag="ACHLAM_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7855664.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8605..9129
                     /gene="ygaP"
                     /locus_tag="ACHLAM_004215"
     CDS             8605..9129
                     /gene="ygaP"
                     /locus_tag="ACHLAM_004215"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO7855665.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9176..9580)
                     /gene="stpA"
                     /locus_tag="ACHLAM_004216"
     CDS             complement(9176..9580)
                     /gene="stpA"
                     /locus_tag="ACHLAM_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO7855666.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2317 a         2560 c         2710 g         2375 t
ORIGIN      
        1 atttttagat tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga
       61 tcgcttctga tgaatgcact gaccgccgta caaaataacg ctgtcgattc aggccaggac
      121 tatagcggat tcactctcat cccgtcggcg caatctccgc gtctgctgga actcaccttc
      181 accgaacaga cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg
      241 gagtacaaat cttttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaac
      301 cagctgcaac cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc
      361 aatgcggtgg gtgtcgatga tgtgaagcaa gcggatgaga tggtgaagct ggcaacggcg
      421 gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcga
      481 ttcgtggtga aaaatgttga taactcagac agctatctgc gtcagccgca ccgcgtaatg
      541 gagctgcaca acgacggcac ctacgtcgaa gagatcaccg actatgtgct gatgatgaaa
      601 atcgacgagc agaacatgca gggtggaaat tcgttgctgc tgcatctcga tgactgggaa
      661 catctggacc actatttccg ccacccgctg gcgcgtcgcc cgatgcgctt tgccgcgcct
      721 ccgagcaaaa acgtcagcaa agatgttttc catccagtgt tcgacgttga tcaacagggt
      781 cgcccggtga tgcgttatat cgaccagttc gtccagccaa aagacttcga agaaggcgtg
      841 tggctgagtg agctttccga tgccattgaa accagcaaag gcattctttc tgtgcccgtt
      901 cccgttggca aattcctgtt gattaacaat ctattctggc tgcacggtcg cgaccgcttt
      961 actccgcacc cggatctgcg ccgtgaactg atgcgtcagc gtggctattt cgcttacgcc
     1021 actaaccact accagacgca tcagtaagcg caaaggaatt gagcggatgt atgattttgt
     1081 gattattggc ggcggcatca tcggcatgtc gaccgccatg caactgattg atgtttatcc
     1141 ggatgcccgc attgcgttgc aggaaaaaga gtccggcccg gcctgtcacc agacgggcca
     1201 caacagtggc gtgatccatg ccggggtcta ttacacgccc ggtagcctga aggcacagtt
     1261 ttgcctggcg ggaaaccgcg ccaccaaagc cttttgcgat caaaacggca ttcgctatga
     1321 caactgcggc aagatgctgg tcgccacctc tgaactcgaa atggaacgga tgcgcgcgtt
     1381 gtgggaacgc acggcagcga acggtatcga gcgcgagtgg ttaaacgccg aggaactgcg
     1441 cgagcgcgaa ccgaatatca ccgggctcgg cggcattttt gtgccgtcca gcggcattgt
     1501 cagctaccgc gaagtgacgg cggcgatggc aaaaatcttc caggccagag gcggcgagat
     1561 tatttataac gccgaagtca gcgccctcag tgagcataaa aacggcgtgg tgatacgtac
     1621 ccgtcagggc ggtgaatatg aagcatcgac gctgattagc tgttccgggc tgatggctga
     1681 ccggctggtg aaaatgctcg gcctcgaacc gggctttatt atctgcccgt tccgtggcga
     1741 gtatttccgc cttgcgccgg agcataacca gattgttaac cacctgattt accccattcc
     1801 cgaccccgcg atgccatttt tgggcgtgca tctcacccgc atgatcgatg gcagcgtgac
     1861 cgtcgggcca aacgcggtgc tggctttcaa acgcgaaggc tatcgtaagc gcgacttctc
     1921 atttagcgac acgctggaga ttttgggctc gtcggggatt cgccgggtgc tgcaaaacca
     1981 tctacgctca ggactgggcg agatgaaaaa ctcgctgtgc aaaagcggct atctgcggct
     2041 ggtgcaaaag tattgtcccc ggctttcgtt aagcgatctc cagccctggc ccgctggtgt
     2101 gcgggcgcag gcggtatcgc cggacggcaa gctgattgac gattttctgt ttgtcaccac
     2161 cccgcgcacg atccacacct gcaatgcgcc ctccccggca gcgacatcag caattcctat
     2221 tggtgcgcat attgtcagca aggtacaaac gctgttggca agccagagta accccggacg
     2281 cacgctgcga gcggcacgta gtgtggatgc cttacacgcc gcatttaatc aataaccttt
     2341 gaaaacagga cgtaacaatg aaacctaacg acagtaactt attccgccag caggcgttga
     2401 ttaacgggga atggctggac gccaacaatg gcgaggtcat cgacgtcacc aatccggcga
     2461 acggcgacaa gctgggtagc gtacccaaaa tgggcgctga tgaaacccgc gccgctatcg
     2521 acgccgccaa ccgcgctctg cccgcctggc gtgcgctcac cgccaaagaa cgcgccacca
     2581 ttctgcgcaa ctggttcaat ttgatgatgg agcatcagga cgatttagcg cgcctgatga
     2641 ccctagaaca gggcaaaccg ctggccgaag cgaaaggcga aattagctac gccgcctcct
     2701 ttattgagtg gtttgccgaa gaaggcaaac gcatttatgg cgacaccatt cctggtcatc
     2761 aggccgataa acgcctgatt gttatcaagc agccgattgg cgtcaccgcg gctatcacgc
     2821 cgtggaactt cccggcggcg atgattaccc gcaaagccgg tccggcgctg gcggcaggct
     2881 gcaccatggt gctgaagccc gccagtcaga cgccgttctc tgcgctggcg ctggcggagc
     2941 tggcgattcg cgcaggcgtt ccggctggcg tatttaacgt ggtcaccggt tcggcgggcg
     3001 cggtgggtaa cgaactgacc agcaatccgc tggtgcgcaa gctgtcgttt accggctcga
     3061 ccgaaattgg ccgccaatta atggaacagt gcgcgaaaga catcaagaaa gtgtcgctgg
     3121 aactgggcgg caacgcgccg tttatcgtct ttgacgatgc cgacctcgac aaagccgtgg
     3181 aaggcgcact ggcctcgaaa ttccgcaacg ccgggcaaac ctgcgtctgc gccaaccgtt
     3241 tatacgtgca ggacggcgtg tatgaccgct ttgccgaaaa attacagcag gcggtgagca
     3301 aactgcacat cggcgacggg ctggagaatg gcgtcaccat cgggccgctg attgatgaaa
     3361 aagcggtagc aaaagtggaa gagcatattg ccgatgcgct ggagaaaggt gcgcgtgtgg
     3421 tttgcggcgg taaagcggat gaacgcggcg gcaacttctt ccagccgacc attctggtgg
     3481 acgttccggc caacgccaaa gtgtcgaaag aagagacgtt cggccccctt gccccgctat
     3541 tccgctttaa agatgaagcc gatgtgattg cgcaggccaa tgacaccgag tttggcctgg
     3601 ccgcctattt ctacgcccgt gatttaagcc gcgtcttccg cgtgggcgag gcgctggagt
     3661 acggcatcgt cggcattaat actggcatta tttccaatga agtggccccg ttcggtggca
     3721 ttaaagcctc gggtctgggt cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa
     3781 tcaaatatat gtgcatcggt ctttaactgg agaacgcgaa tgagcagcaa taaagagtta
     3841 atgcagcgcc gcagtcaggc gattccccgt ggcgttgggc aaattcaccc cattttcgct
     3901 gaccgcgcgg aaaactgccg ggtgtgggac gttgaaggcc gtgagtatat tgatttcgcg
     3961 ggcggcattg cggtgctcaa taccgggcac ctgcatccga aagtggttgc ggcggtggaa
     4021 gcgcagttga aaaagctgtc gcacacctgc ttccaggtgc tggcttacga accgtatctg
     4081 gagctgtgcg agattatgaa tcagaaggtg ccgggagatt tcgccaaaaa aacgctgctg
     4141 gtaacgaccg gttctgaagc ggtggagaac gcggtgaaaa tcgcccgcgc cgccaccaaa
     4201 cgcagcggga ccatcgcctt tagcggcgcg tatcacgggc gcacgcatta cacgctggcg
     4261 ctgaccggca aggtgaatcc gtactctgcg ggcatgggac tgatgcctgg gcacgtttat
     4321 cgcgcgcttt atccttgccc gctgcacggc atcagtgaag atgacgctat cgccagcatt
     4381 caccgaattt ttaaaaatga cgctgcgccg gaagatatcg ccgccatcgt gattgagccg
     4441 gttcagggcg aaggcggttt ctacgccgcg acgccagcct ttatgcagcg tttacgcgcg
     4501 ctgtgtgacg agcacgggat catgctgatt gccgatgaag tgcagagcgg cgcggggcgt
     4561 accggcacgc tgtttgcgat ggagcagatg ggcgttgcgc cggatcttac cacctttgcg
     4621 aaatcgatcg cgggcggctt cccgctggcg ggcgtcaccg ggcgcgcgga agtgatggat
     4681 gccgtcgctc caggcggtct gggcggcacc tatgccggta acccgattgc ctgcgtggca
     4741 gcgctggaag tgttaaaggt attcgagcag gaaaatctgc tgcaaaaagc caacgacctg
     4801 gggcataagc tgaaagacgg attgctggcg atagccgaaa aacacccgga gatcggcgat
     4861 gtacgcgggc tgggagcgat gattgccatc gagctgtttg aagatggcga tcacagcaaa
     4921 ccggacgcca aactcaccgc cgagatcgtg gcgcgtgccc gcgataaagg cctgattctt
     4981 ctctcctgcg gcccgtatta caacgtgctg cgcatccttg taccgctcac cattgaagac
     5041 gctcagatcc gtcagggtct ggaaatcatc agccagtgtt ttgatgaggc gaagcagtag
     5101 atccgtaccc atgccggagg cgacgctgcg cgtcttgtcc ggcctacgcg aatccaggtc
     5161 ggataaagcg tttacgctgc atccgacaat ctgtacgtga acaggaataa atctatgtcg
     5221 gccggataag gcggagccgc tctccggtaa aaagaaccaa taacaattac acgcgtgacc
     5281 cgacgcggga aatgtcgggg cgctctccca agtgacacac tttcgagagg attcaggatg
     5341 gggcaatcat cgcaaccaca tgagttaggc ggcgggctga agtcacgcca cgtcaccatg
     5401 ttgtctattg ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc
     5461 gaagcgggcc cggcggtatt actggcctat ctgttcgccg gattactggt ggttatgatt
     5521 atgcggatgt tggcggaaat ggcggttgcc acgcccgata ccggttcgtt ttccacctat
     5581 gccgacaagg caattggccg ctgggcgggt tataccatcg gctggctgta ctggtggttt
     5641 tgggtactgg ttatcccgct ggaagccaac atcgccgcca tgatcctgca ctcgtgggtt
     5701 ccaggcattc ccatctggtt attttccctg gtcattaccc tcgccttgac tggcagtaat
     5761 ttattaagcg ttaaaaacta cggcgaattt gagttctggc tggcgctgtg caaagtcatc
     5821 gctatcctgg cctttatttt ccttggtgca gtcgcaatta gcggttttta cccttatgcc
     5881 gaagtgagcg ggatctcaag attgtgggat agcggcggct ttatgcccaa cggcttcggc
     5941 gcagtattaa gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcacc
     6001 attgccgccg cggaatctga cacgccggaa aaacatattg tccgcgccac caactcggtt
     6061 atctggcgta tttctatctt ctatttatgc tctatttttg tcgtggtggc attaataccg
     6121 tggaatatgc cggggctgaa agccgttgga tcttatcgct cggttctgga gttgctcaat
     6181 attccccatg cgaaattaat catggactgc gtgatattac tttccgtaac cagttgcctg
     6241 aactcggcgc tgtataccgc ttccagaatg ctctactcct taagccgtcg cggtgatgct
     6301 cccgcggtaa tgggtaaaat caaccgcagt aaaaccccgt atgtggcggt attactctcg
     6361 accggtgcgg catttttaac ggtggtggtg aattattacg cgcctgcgaa agtgtttaaa
     6421 tttctgatcg atagctccgg cgctatcgcc ctgctggttt atttagtcat tgccgtttca
     6481 caattgcgga tgcgcaaaat tctgcgagca gaaggaagcg aaattcgcct gcggatgtgg
     6541 ctttatccgt ggctcacctg gctggtcatc ggttttataa cctttgtgtt ggtagtgatg
     6601 ctattccgcc cggcgcaaca gttagaagtg atttccaccg gtttattagc gatagggata
     6661 atctgtaccg tgccaattat ggctcgctgg aaaaagctgg tattgtggca aaaaacaccc
     6721 gttcataata cgcgctaata atgatcagga gtcacaccat gaccattacg tctctggatg
     6781 gctatcgctg gctgaagaac gatattattc gcggaaactt tcaaccggat gaaaaattac
     6841 ggatgagttt gctgacatcg cgttatgcac ttggcgttgg gccgttgcgg gaagcgcttt
     6901 cgcaactggt agcagaacga ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt
     6961 ctatgtcaga gcaagagctg ctcgatattt tcgacgcccg cgccaatatg gaagcgatgc
     7021 tggtcagtct ggcgattgcc cgcggtggcg atgaatggga ggcagacgtt ctcgcaaaag
     7081 cgcatctgct cagtaagctg gaggcctgtg acgccagtga gaaaatgctt gatgagtggg
     7141 atctgcgtca tcaggcgttt catactgcaa ttgtggcggg ctgtggttct cactatttgc
     7201 tgcaaatgcg tgaacggttg tttgatctgg cggcgcgtta tcgatttatc tggctgcggc
     7261 gaacagtgct ttcggtggaa atgctggaag ataaacgcga tcagcaccag accctcactg
     7321 cggcggtact ggcgcgagat accgcgcgcg ccagtgagtt aatgcgccag catttactga
     7381 cgccaattcc cattatccag caggcgatgg ctggcaatta actactcttc aggaatacgc
     7441 aacacttgcc ccgggtaaat tttatccggg cttttgagca tcggtttatt cgcttcgaag
     7501 attttattgt acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg
     7561 ccagacttaa cggtataaaa ctggctggca gcggctggtg tcgccgtttt cacctgatca
     7621 tcgacactgg caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc
     7681 tgacttaagc cgtcacccgt gaccgtcgct ttgccgtcgg cgatttgaat gttcacttta
     7741 tcggcatcag gtattccggt tttgttcaga tgctcctgca ccttcttcgc ctgatcgtct
     7801 ttatcgtgct gacctgtaac cgcgtcccag agtttttctc cggcatcttt cacaaaattg
     7861 aacagaccca tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac
     7921 gcggcgcgtt aacgccgcgg gggtgaacac aggtttaatc gcgggtttgc acccagaacg
     7981 cgtgaatcaa accggggata tacccaagca gtgtcagcag aatattaata atgaacgccc
     8041 aaccgaaccc tttaccgagc agcacgccga gcggcggcag aatgatggtg atgacgattc
     8101 tccagaaacc catatgtact ccctataaga aaattactca ttgtttaaaa agagatttta
     8161 tctcttaagc gtagtaattt tagcggagcc tgccagtttc agcttacgct tttactctca
     8221 tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttact taatttagaa
     8281 aatacttaaa tatctatgac tgaactcgcg caattacagg ctagtgccga acaggcagcg
     8341 gccttattga aagcaatgag ccaccctaaa cggttgctga ttctgtgcat gcttagcggt
     8401 tcccccggca ccagtgcggg agagctgacg cgcattaccg gactgagtgc ctctgcaaca
     8461 tcacagcatc tcgctcgtat gcgggacgaa gggcttatcg acagccaacg ggatgcccaa
     8521 cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat
     8581 gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa
     8641 gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga atatcttcgt
     8701 gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc aggtcttccg
     8761 gctaaattac gtcgcgagca aataatattc cactgccagg ctggtaaacg caccagtaat
     8821 aacgccgata aattagcagc gattgccgcc cccgcagaaa tctttttact cgaagatggg
     8881 attgatggct ggaaaaaagc aggattacca gtagcggtaa ataaatctca acccttgccg
     8941 ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taatcggcgt tatactaggt
     9001 tataccgtaa atagcggttt cttcttatta agcggctttg ttggtgccgg gttactgttt
     9061 gcaggaatta gcggtttttg tgggatggca aggttgttag ataagatgcc gtggaaccaa
     9121 cgagcttgag aagcgacgcc ggacgcgcca tagcagcaac atccggcctc agtaattaga
     9181 tcagaaaatc gtcgagagat ttaccttctg ccagcgcctg agcgattggc ttcggtgtac
     9241 ggccctgacc ggtccaggtt ttagtttcac cgttaacatc gatgaattta tatttcgccg
     9301 gacgcggctg gcgtttttta ccagcgcgtg gtgccgcagc agagctatta cccaataact
     9361 cttccgggtt aattccgtca gctttcatca gctccagcca ggtgctaatt ttttcctggc
     9421 gctctgccag ttcacgctgc tgttgttctt cttcttcacg tctttcttta gtgacaaccc
     9481 tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga
     9541 gggtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac
     9601 gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat cacttattat atttccacaa
     9661 aatttaatct atttacagtc ctgcacgaga ttatttcagc tgttattttc tgaaaccctc
     9721 agaataattt caagattata tattagccat tttgatgagt aatttaggtt attggtgaac
     9781 agagcggcgc tctctgctcg ctaattccgc aagcccgaca aaaccaaagt gaataaaaat
     9841 gcaactcaac agaataaaag aaaacatcta tcactggtgc tttctgctgt caatgtacat
     9901 tatctggcat aaaaaatgct gaaaatgacc cgatcactac atacaccgaa ttctctacgc
     9961 tt
//
LOCUS       DAZGAP010000181         9647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGAP010000181 DAZGAP010000000
VERSION     DAZGAP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.182"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(119..1606)
                     /gene="yeeO"
                     /locus_tag="ACHLAM_004217"
     CDS             complement(119..1606)
                     /gene="yeeO"
                     /locus_tag="ACHLAM_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO7855667.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVVMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1767..1842
                     /locus_tag="ACHLAM_004218"
     tRNA            1767..1842
                     /locus_tag="ACHLAM_004218"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1800..1802,aa:Asn,seq:gtt)
     gene            complement(1880..2830)
                     /gene="cbl"
                     /locus_tag="ACHLAM_004219"
     CDS             complement(1880..2830)
                     /gene="cbl"
                     /locus_tag="ACHLAM_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO7855668.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2932..3849)
                     /gene="nac"
                     /locus_tag="ACHLAM_004220"
     CDS             complement(2932..3849)
                     /gene="nac"
                     /locus_tag="ACHLAM_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO7855669.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4176..4251
                     /locus_tag="ACHLAM_004221"
     tRNA            4176..4251
                     /locus_tag="ACHLAM_004221"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4209..4211,aa:Asn,seq:gtt)
     gene            complement(4306..5241)
                     /gene="ldtA"
                     /locus_tag="ACHLAM_004222"
     CDS             complement(4306..5241)
                     /gene="ldtA"
                     /locus_tag="ACHLAM_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7855670.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5303..6382)
                     /gene="cobT"
                     /locus_tag="ACHLAM_004223"
     CDS             complement(5303..6382)
                     /gene="cobT"
                     /locus_tag="ACHLAM_004223"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO7855671.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPISGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6394..7137)
                     /gene="cobS"
                     /locus_tag="ACHLAM_004224"
     CDS             complement(6394..7137)
                     /gene="cobS"
                     /locus_tag="ACHLAM_004224"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO7855672.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7134..7679)
                     /gene="cobU"
                     /locus_tag="ACHLAM_004225"
     CDS             complement(7134..7679)
                     /gene="cobU"
                     /locus_tag="ACHLAM_004225"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO7855673.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7813..8250)
                     /locus_tag="ACHLAM_004226"
     CDS             complement(7813..8250)
                     /locus_tag="ACHLAM_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO7855674.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLNGGSVQSLSDAYSDYSVLLLIAAESEISVPEWLPHSEGTTVIVIAEHNARLLTGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(8468..8643)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8732..9646)
                     /gene="pocR"
                     /locus_tag="ACHLAM_004227"
     CDS             complement(8732..9646)
                     /gene="pocR"
                     /locus_tag="ACHLAM_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFO7855675.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
BASE COUNT         2649 a         2591 c         2079 g         2328 t
ORIGIN      
        1 cgactgaact acagaggaat cgtgagaacg aggcgaatat tagcgatgcc catccacaat
       61 gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt
      121 attccgacac aactggcttt ttttcacact tttgcggctc gggtcgaggg tatttccaca
      181 gccaacgtcc agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa
      241 acattcccat ccagacacca accacacccc agccaagcat gattcccagc acataaccga
      301 ctacaacccg gcaaccccac atgctcaaca tagaaaccca catggcgtaa cgtgcatcgc
      361 gagccccctt aaatccagcg ggtagcaccc atgaggcgga ccaaataggc ataaataaag
      421 catttagcca aatcagaatc acaacgacgt gtttaacctg tggatcctgg gtgtaaaacg
      481 atgccataac cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg
      541 acagccagaa cacatgccgc aactgaatct ctgcttgcgc tatctgccct actcccaacc
      601 ttcggcctgt aatgatcgta gaggcagaac cgagcgcact tccgggtaag ttgataagag
      661 ccgcaattga gaacgcgata aaatttccgg caataacgct ggtccccatc ccggcaacga
      721 acatttgagt taataaccga ccactggtaa ataacactga ttcgacactc gctggaatac
      781 caatccccat gacttcccag ataatgctaa aattcagcgg tttaaaatag ctctttaacg
      841 aaatccttag cgcaggatta aaaccaatcg ccagcaccca caaaattgca actgcgccaa
      901 tataacgaga aatggttaaa cccaacccag ccccgacaaa tcccagtccc ggccaggaga
      961 aaaggccgta aatcaatatg ccgctaataa taatattaag aatattcagg ctaccgttaa
     1021 tcagtagcgg tattttcgta ttccctgcac cacgaagtgc cccgctacca ataagagtga
     1081 tggcagctgc tggataactg agtacggtca gctccagata agtcaacgcc agtgctttaa
     1141 cttctgtcgt ggcatcaccc gcgacgaaat caataatttg ttcgccaaaa tgatgaatta
     1201 gcgttgccaa cagtacggca aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc
     1261 tcgctcgtcg tcgatctcgc ttaccgagac taaatgctac aacgacagta gtaccaagat
     1321 cgatagctgc aaaaaaagcc ataacgacca tattgaagct gtccgccaac cccacgccgg
     1381 ccatcgcatc ttttcccagc cagctgacca gaaaagtgct caggaccccc atcaacagga
     1441 cacaggcgtt ctccatgaag ataggaacag caagcggggt tatctcgcgc cagaacaaca
     1501 ctttgtagct cttgcgttta gagtgccagc gagtgccgtg aacaacctgg cgtaaagcag
     1561 aggagatatt caaagccgac cttaattgca gaaagtgaaa ccacatttca aatgatgagg
     1621 gagaatcagc aaagctgcaa agattttcgc caacaaattg tctgcaaatg caacaaactg
     1681 ttgatagaaa cggcaaacag ttgggaaatt taaaaatcgg gtttgacaaa atatttttcg
     1741 ccgttaagat gtgcctcaac agcgattcct ctgtagttca gtcggtagaa cggcggactg
     1801 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaatttctg aaaagcccgc
     1861 ttttatagcg ggctttttgc tatatctgat aatcaatttc ctcttcactg ttttccatca
     1921 cctggcgctt gatatcctca actgacagtc ctgcattaca aagttccaga aagcgccaga
     1981 catagttacg ctgaagttgc cctcgcttca gtcccaacca gacagtatta gcatcgaaaa
     2041 gatgccgcgt atccaggcgg attaaattct tttcctcttg ttcgccgctg gattgttcgg
     2101 caactaatcc gatcccaagc ccaagagcaa cataggtttt aatgacatca gaatcctgtg
     2161 cacttaatac aatatctgcc ggcaaacctt tgcgggcaaa tgcgtcatca atgcgtgagc
     2221 gccccgtaat tccctgtcgg taagtaatta acggccactt cgctattgat tccagcgtca
     2281 atggtgtaat ttgcgttaag ggatgatcga gtggaacaag caaactatgg tgccagcgaa
     2341 accacgggaa ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg ataccaatat
     2401 cagcttcgcc attttgcaac aacgtcgcaa tttcctgtgg cgtaccctgg attagctcga
     2461 gccgaacctc cgggaaaagt tcgcgaaaag ctttaatgac ctctggcaag ctataacgtg
     2521 cctgagtatg cgtcgttgca atagtgagaa caccagacgt atcgttggta aacaggtctg
     2581 caagccgacg aacattactg gcttcattca gaatacgttc tgcaatgacc agtaatgctt
     2641 tgcccggttc agtcatgccc agcagtcgct tacctcgtcg aacaaatatt tcgatgccaa
     2701 gttcatcctc cagttcccga atatgacggc taacgcctga ctgtgaggta aaaagcatat
     2761 tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc gcggattatc tttagttgtt
     2821 ggaaattcac ggtaaactcc gggcagttca gatatcccgt tattgttaaa gtctaatgcc
     2881 cggcataaca aataataaaa acccgcatct tattccatcc cgatataacg cttagctcac
     2941 caattgccac tgcctttttt ccatcactgg agaactaatc actgacatta acaactcttt
     3001 cactgcctgt gcctgtggtg acaagtttgc tctggcgggt aaatttaagg acaaagagag
     3061 actcattgaa ggagtggtaa tgcgtgacat ccacccattt actgcgccac ataacgaacg
     3121 cgcagccgat tcaggtaata ctgcaacgcc catgccgctg gcaatcgctg cggtaagcgt
     3181 ggcaatagac tcaatttcac caatgacttt tgccgtgagc cgccgtaggg aaaaagcctc
     3241 atcaacacga agtctaacag cgctgtaatc actggggaga aaaaggttca tttgcgcaat
     3301 agcattaaca tcaacgcttt gccccgggca atcttgagtt cctaccagga aaagatcttc
     3361 tttcagcaaa gcctgactgg ataccccagc cacaggggaa tgctcataaa tcaccgccat
     3421 atcgagttgg tgatttatca gtttttcgtt aagcactgca ccactatttt catgaagata
     3481 gataacgacc tccgggaatt cagcgcgaac cgcctgtaat aagggcatgg tgatggatga
     3541 cgcagcggtt cctggcgcaa agccaatcga gacttgcccc gataatgact gaccaacgtt
     3601 atgcaccgcc agttgggcct gctcacactg acgtaaaatg gcccgtgcat gggtatagag
     3661 aatttttccg gcgtctgttg gcgtaacgcc ccgttttgtg cgaatcaaaa gctgttgatt
     3721 taactcacct tccagtgtgg caacctgctg gctgagcgct ggttgtgcga tatgcaatac
     3781 ttcagcagcc tgggtcaggc taccaatatc tacaattttt acgaagtatt tcaggcgtct
     3841 gaagttcatg ttgcctccgg tttttaagaa tcggcccaag tgccgccatt acttacaacc
     3901 agaatgcaag atgcttgcca gttctatttt agcgttgatg cgcaagctaa ccaactgtca
     3961 aataagagat tatgatagat tcgtcattgg ccctttttat cagcagtcgc attcacctgc
     4021 cctataaaag gagggtatgc accacgatgg ttcattaccc aataagattg aaagctcacc
     4081 actttgttga aattggcagc aaacaaacaa aaaaatgcat ttcacccttt gacatcacca
     4141 tgcactgcca ttaatatgcg ccccgttcac acgattcctc tgtagttcag tcggtagaac
     4201 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattcaaaa
     4261 aagcctgctt cgagcaggct ttttgctttc taattaccaa cgctcttaaa acatctgtct
     4321 tgaaccagaa ctaatttgca caggcattcc cgatcgatgt tgcaacgcag catttgcgcg
     4381 atttacatca acttcttgcc cgttgataaa cgcccgcaaa gatggggtta ccggcaatgg
     4441 cacttttcgg tcagactcat attctgcacg attgcgcgac agcggctcat gaacttccag
     4501 ccactttgaa ccatccggtt ccgttgtgta tttcactggc tgatcaataa tttgtacacg
     4561 agtgcctaca ggaacattat caaacagata tttgatatcg tcattgcgca gacgaataca
     4621 gccctgactt acccggagcc caataccaaa attggcattg gtaccgtgga tggcatacaa
     4681 cctgccaata taaatcgcgt acagccccat ggggttatcg ggccccgcag gaacaaatgc
     4741 gggcaaactc tcccctcgtt tcgcatattc gcgccgagtg ttcggcgttg gcgtccaggt
     4801 tggtgcttct tgtttacgtt caacggtagt cacccagtta cgcggggttt ctcgcccagc
     4861 ctggccgata ccaataggaa agacttccac tgtattactg tctggcggat agtaataaag
     4921 acgcatttca gcgacgttaa ccacaatccc tttacggaca gtggcgggca aaatcagttg
     4981 ctgcggaatg gtgagttgtg agccagactt cggcaaaaaa acatcagcgc ccggattcgc
     5041 ttccagcata ttacttaacc cttgcccgta ttgtgcggca aaagtctcca gcggctgagt
     5101 attgtgatca ggaacagtta cagtaagcga ctgccccact aaacggctac cctctggagg
     5161 taatggataa gttaccgcca ggctattttg gctggcaaaa agcagagcaa atgagcaaag
     5221 aatatttaca cgacgcatca tgtccctttc ctaagtcgcg aatactatcc gataagtata
     5281 gcttttatca gacttttcgt ttttagctgt tcaaatcaga agtcgtattc cccggtagaa
     5341 caatattact ggcagcaagt tcgcccatat tgttgtatat cgcacaagca gcttcgatga
     5401 tgggcatcgc cagagctgca ccactcccct cacctaaacg catatccata ttgagataag
     5461 gctccagccc caaatgcgac agcgctatac gcgcgccttt ttctgccgac aagtgagaag
     5521 gaatgagata cggtttgatt gcaggagaca tctggcaggc tgcgagcgca gcagcataag
     5581 aaagaaatcc atccagcaac acgggtaaac cgcaggaagc agcacctaac atcactccag
     5641 ctattccgac caaatcaaat ccacccactt ttgccaggac atcaacacca tcccgaggat
     5701 ttggttgatt caacgtaatc gcccgacgca caacatcaat tttattagcc agtttatctg
     5761 tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc ccggccagtg attgtgctga
     5821 ctattgccgc tgccggtgtc gtgtttgcca tccccagttc acctacacca aacagcgtga
     5881 caccgttttt tgccagctcc cgcgtataac atatgacgtc caaaagcaac ttttcagcct
     5941 gacggcgact cattgccgga gctgaagcaa tattgccgct acctcgtgcg acacgcatgt
     6001 tgataagccc ggagataggc tcagcagtat caataccaac atcaattacg tggacgttag
     6061 cgcccgcttg tgctgccagc acacacacgc cggttgttcc gcgggtcata ttttcagcct
     6121 gtatggctgt cacttctttt ggagaaatag cgaccccttc ctcccagacg ccgtgatcgg
     6181 cacacataac cagtatcgct tttttgccca catgcggtat gccattcaac cccggcattc
     6241 ctgccagttg tatggcaagc gcctccagct ttcccaggct accaacaggt ttgagtaacc
     6301 cgtcaatatg ccgttgtgca cgcgacatag cggcaggatc gatggctggg atcgtattca
     6361 gtaaatcggc aagtatttgc atgtcacgtc tctttatagc agtgccagca ggaaaaccag
     6421 ttcaccaagc tctatggctg ctcccagcgt atcgcccgtt tgcccgccca gcgtacgttt
     6481 gagaagctga ccgaggatga aaatcaccac catcgtcacc accatagcag ccacaccatg
     6541 catacccggc aataatacgg cagcaaaaat agcggctaag ccgagtgtga cgcaggtttg
     6601 tcgcccatca attttgccaa tgaacacatt gccaagccct tcttcccgcg cataacgatg
     6661 acgatacatc aataatgcgg cagtcccgcg gctgaccgca catgctgccg ccagcgatgc
     6721 aaggatcggt tcgccacgca gagtcaactc gcttaacacc agaatcttta ccagtaccac
     6781 aaaaatcaat gccagaccgc cgtgggtgcc taaacggcta tcacgcatga tttccaacat
     6841 gcgatcgcgg ctacgtgcag aaaatacgcc atcacaggta tcagcgaggc cgtccagatg
     6901 gaatcccccg gtcatcagcg ccagcacaag tacgctaaac agtgccgcca gtggtacgcc
     6961 acaccatgcc tgcagcacca tgaagaccag cccgctaatc gcgccaagca ataatccaat
     7021 caaaggaaaa gtaataatac cgcgagaata atgctcgaaa tccagtccct gggaccagcg
     7081 acgcggtaca ggcagacgcg taataaatga gagcatcgcc caaaataatt tactcatttg
     7141 atttttactc caatacccga aaccaccagc catacttcat ttgccgcagc ggccaactgc
     7201 tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca gacgactctc cggcacaatc
     7261 cccattccca cttcgttagt cactaataca acctttgcgg ggcaacgttg gcaggcagca
     7321 atcaacgact gaatctcagc attaatcgcc tgttccatcg cctgataatt ccattcatca
     7381 gggtctttat cgccgccata atcaaacaac agattagtca ccattgtggt aacgcattcc
     7441 agcaacacag cctcattcgg gttaatgtct gcatgaatta attcatcaag atgttgccaa
     7501 cgctccactg tgcgccagtg ctccgggcgg ccttgccgat gatgttctat ccgtgcagcc
     7561 atctcatcat caaggatttg cgaggtggcg atatacagaa cctgtgaaga gtccccaata
     7621 agcgcctctg cgtggcgact cttcccgctc cgtgcgcccc ccgtcaccaa aatcatcata
     7681 ccgactcctg attcaattgt atgatggtat tcattgaaca gaacaatgga tcccccaccg
     7741 ttgctgaccg caaaacattt acgcgattaa tactgcgcgt aatataatta aaaatccagc
     7801 attctcaatc cattacgaaa gaagatggca ttgcggaata gccagacttc ctcgtgttaa
     7861 cgtccgacaa aatgcgtcat tgacagcggt ttctctggca tgacagattt cattcagata
     7921 atggatcaat gccacagccc caccagccgg accagtcagc aaacgggcat tgtgttctgc
     7981 aatcacaatt accgtcgttc cctcagaatg agggagccat tctggtacgg atatctcgct
     8041 ttcagctgct atcaatagca gcactgaata atcgctatac gcatctgata atgattgcac
     8101 agacccaccg ttaagaggct ggaaatcttg ctgccagtcc tcaccaacaa aacaggttat
     8161 tggcaatatt tcagccagat gtttcgccag gtccacatct ccagataaga ggcaaaaaag
     8221 agctatcgat tcgggtttta tcgttttcat gcctctcgcc ctccacttag tttcctgtaa
     8281 tgtcggtctg gttgatgagc catgaaacaa gattgccaca ccttaagtgc agccctggtg
     8341 cctgtcggca ctgcgatgaa agaactaaat gatgccgcca taccctgatc ctttcagggt
     8401 ggtaggggta taaagacgcg ccggtactct accggacgct tccgggcaac ccacttccca
     8461 ccgaagttgt tggtattgac tgacagactg gtcttctggc ttagcgtcat cctcccccga
     8521 ccttcccaat ctcgcgatca gtggtatttt cgggtttgtc agcattacag cagcgggggc
     8581 tgcgagggat tttcaccctc ttcccaggca caaatgtgcc acacctgtca gcgtatgtta
     8641 tgagctgatt tcacttttcc cataacacgt aaataacgtt aattattcat tccagcgttt
     8701 atttaaacct tccctttttt tagcatttac tctataccgt ttccatcgcc gcattttcac
     8761 tcacttgctg tcgatacacc tgcggcgtaa cctgataagt ctgacggaaa actttgcaaa
     8821 aataactggt ttgcgaaaaa cctaaattac gcgcaatgct ggcgatgctc cagtcactat
     8881 ggcacagtaa ctcacgagcg ctggccatcc gttgttggtt tacccacgca ttaaaaccga
     8941 ttccatggta tttcttgaat aacttactga aataataagg acttagataa acgtgcgagg
     9001 cgacatcttc cagacgcaaa tcgtctgata aatgcgcatc aatatagcgt aaagcttttt
     9061 tcattttatt gtcgtgggga ttaggcgcac gagtctggcg caccgaatct gactgagatt
     9121 tgttctcttt aataaccaca aaattgagtt gttttttgag gcaattttcc acaatcaact
     9181 tcaacaaatc agcagaggcc ataactcttg agtaatccat ctcaggaact ttgcggaatt
     9241 cattaagtaa ttcgggatct gcctgccagc aatcatcgac attcagaata tcgaccaaat
     9301 cgacatcatc attacgcaga cgaacttgcc cgcacaatac aaaaccaact aaatgtccgg
     9361 cgattaccag tggaatagag aaatcggtta atccggcatg acagcgataa atacaaggct
     9421 gatcggattt agaggcttcc agacccccac aacgatcgct catgcgacat cgagtgctca
     9481 gctgagggtg ttgccgcatc atctgacaaa atggagtgaa gttaaacagc tcggaaattt
     9541 catcgccgtg aatattgacc acgacaaccg caaggctggt cgcctgcgca aaatcctgcg
     9601 cgattttatt aatgagttct gaattaagtg tcgccgcaga aatcatg
//
LOCUS       DAZGAP010000182         9617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGAP010000182 DAZGAP010000000
VERSION     DAZGAP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.183"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..1038
                     /gene="cysB"
                     /locus_tag="ACHLAM_004228"
     CDS             64..1038
                     /gene="cysB"
                     /locus_tag="ACHLAM_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO7855676.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEIMFKDIKLPEK"
     gene            1357..1497
                     /gene="ymiA"
                     /locus_tag="ACHLAM_004229"
     CDS             1357..1497
                     /gene="ymiA"
                     /locus_tag="ACHLAM_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO7855677.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1500..1667
                     /gene="yciX"
                     /locus_tag="ACHLAM_004230"
     CDS             1500..1667
                     /gene="yciX"
                     /locus_tag="ACHLAM_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO7855678.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQQKQ"
     gene            1781..1876
                     /gene="ymiC"
                     /locus_tag="ACHLAM_004231"
     CDS             1781..1876
                     /gene="ymiC"
                     /locus_tag="ACHLAM_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO7855679.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2026..4701
                     /gene="acnA"
                     /locus_tag="ACHLAM_004232"
     CDS             2026..4701
                     /gene="acnA"
                     /locus_tag="ACHLAM_004232"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO7855680.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGNITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDDVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4765..5355)
                     /gene="ribA"
                     /locus_tag="ACHLAM_004233"
     CDS             complement(4765..5355)
                     /gene="ribA"
                     /locus_tag="ACHLAM_004233"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO7855681.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5525..6289
                     /gene="pgpB"
                     /locus_tag="ACHLAM_004234"
     CDS             5525..6289
                     /gene="pgpB"
                     /locus_tag="ACHLAM_004234"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO7855682.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6438..6746
                     /gene="lapA"
                     /locus_tag="ACHLAM_004235"
     CDS             6438..6746
                     /gene="lapA"
                     /locus_tag="ACHLAM_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO7855683.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            6753..7922
                     /gene="lapB"
                     /locus_tag="ACHLAM_004236"
     CDS             6753..7922
                     /gene="lapB"
                     /locus_tag="ACHLAM_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO7855684.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8113..8850
                     /gene="pyrF"
                     /locus_tag="ACHLAM_004237"
     CDS             8113..8850
                     /gene="pyrF"
                     /locus_tag="ACHLAM_004237"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO7855685.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8850..9176
                     /gene="yciH"
                     /locus_tag="ACHLAM_004238"
     CDS             8850..9176
                     /gene="yciH"
                     /locus_tag="ACHLAM_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO7855686.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9302..9520)
                     /gene="osmB"
                     /locus_tag="ACHLAM_004239"
     CDS             complement(9302..9520)
                     /gene="osmB"
                     /locus_tag="ACHLAM_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO7855687.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         2379 a         2264 c         2516 g         2458 t
ORIGIN      
        1 tagtcacccg ccaggtgtga cgaaaaaacg atgttctgat ggcgtctaag tggatggttt
       61 aacatgaaat tacaacaact tcgctatatt gttgaggtgg tcaatcataa cctgaatgtc
      121 tcatcaacag cggaaggact ttacacatca caacccggga tcagtaaaca agtcagaatg
      181 ctggaagacg agctaggcat tcaaattttt tcccgaagcg gcaagcacct gacgcaggta
      241 acgccagcag ggcaagaaat aattcgtatc gctcgcgaag tcctgtcgaa agttgatgcc
      301 ataaaatcgg tcgccggaga gcacacctgg ccggataaag gctcgctgta tatcgccacc
      361 acgcataccc aggcacgcta cgcgttacca aacgtcatca aaggctttat tgagcgttat
      421 ccccgcgttt ctttgcatat gcaccagggc tcgccgacac aaattgctga tgccgtctct
      481 aaaggcaatg ctgatttcgc tatcgccaca gaagcgttgc atctgtatga agatctagtg
      541 atgttaccgt gctaccactg gaatcgggct attgtagtca ctccggatca cccgctggca
      601 ggcaaaaaag ccattaccat tgaagaactg gcgcaatatc cgttggtgac atataccttc
      661 ggctttaccg gacgttcaga gctggatact gcctttaacc gcgcagggtt aacgccgcgt
      721 atcgtcttca cggcaacgga tgctgacgtc attaaaactt acgtccggtt agggctgggg
      781 gtaggggtca ttgccagcat ggcggtggat ccggtcgccg atcccgacct ggtgcgcgtt
      841 gatgctcacg atatcttcag ccacagtaca accaaaattg gttttcgccg tagtactttt
      901 ttgcgcagtt atatgtatga tttcattcag cgttttgcac cgcatttaac gcgtgatgtc
      961 gttgatgcgg ctgtcgcatt acgctctaat gaagaaattg agatcatgtt taaagatata
     1021 aaactgccgg aaaaataatt tcttgcgtta ttttcggcac cttttatgta gcgaaggtgc
     1081 cggaatatat tctcttttgt tgcttatttg tataccgtgc acaatatttt tatccctcat
     1141 atttataggg taaattacct gtaaagtagt gtctaattac ccgcctcgca aactcgagtt
     1201 ttcaaatatc tatttcaggt caaaagattg aattattcac tggagacgat tcgtaaattc
     1261 gctggatttt tgactaaagt ttctttagga tttgtctcat cgtatgatta attacaccaa
     1321 tcgttcaacg tcttgctgat aagtgatggc gattatatga ggttagcaat gccttctgga
     1381 aatcaggaac cccgccggga ccctgaatta aaacgcaaag cctggctggc ggtatttctt
     1441 ggttctgctc ttttctgggt ggttgtcgca ctgctgattt ggaaagtgtg gggataacta
     1501 tggtcggtca ggagcaactg gagtcgtcac cattatgtca gcatagtgac aatgaaacag
     1561 aaacgaaacg ggaatgttcc gtcgttattc cagacgactg gcaactaaca tcgcagcagc
     1621 aagcctttat agaactgttt gctgaagatg atcagcagaa acaataatta tcatcattct
     1681 tattacccat ttttaatgaa ttaaagggct tttaatacac cgcagcaata acaggttgag
     1741 ttatctcaac acaaagtaat aaccgttaaa ggtgtagcct atgatcaaca caaatatgaa
     1801 atattggtcc tggatgggcg cattttctct gtcgatgctc ttctgggccg aactcctctg
     1861 gatcattact cactgatccc tgaccccgct gtttgctttg tctcaaggtt tctcctcttt
     1921 tatcaatttg ggttgttatc aaatcgttac gcgatgtttg tgttatcttt aatattcacc
     1981 ctgaagagaa tcagggcttc gcaaccctgt cattaaggag gagctatgtc gtcaacccta
     2041 cgagaagcca gtaaggacac gttgcaggcc aaagataaaa cttaccacta ctacagcctg
     2101 ccgcttgctg ccaaatcact gggcaatatc acccgtctgc ctaagtcact caaagttttg
     2161 ctcgaaaacc tgctgcgctg gcaggatggt aactcggtta ccgaagagga tatccacgcg
     2221 ctggcgggat ggctgaaaaa tgcccatgct gaccgtgaaa ttgcctaccg cccggcaagg
     2281 gtgctgatgc aggactttac cggcgtacct gccgttgttg atctggcggc aatgcgcgaa
     2341 gcggttaaac gcctcggtgg cgatactgca aaggttaacc cgctctcacc ggtcgacctg
     2401 gtcattgacc actcggtgac cgtcgatcgt tttggtgatg atgaggcatt tgaagaaaac
     2461 gtacgcctgg aaatggagcg caaccacgaa cgttatgtgt tcctgaaatg gggaaagcag
     2521 gcgttcagtc gttttagcgt cgtgccgcca ggcacaggca tttgccatca ggttaacctc
     2581 gaatatctcg gcaaagcagt gtggagcgaa ttgcaggacg gtgaatggat tgcttatccg
     2641 gatacactcg ttggtactga ctcgcacacc accatgatca acggccttgg cgtgctgggg
     2701 tggggcgttg gtggcatcga agccgaagcc gcaatgttag gccagccggt ttccatgctt
     2761 atcccggacg tagtgggctt taaactcacc ggaaaattac gtgaaggtat taccgccaca
     2821 gacctggttc tcaccgttac ccaaatgctg cgcaaacatg gcgtggtggg gaaattcgtc
     2881 gaattttatg gtgatgggct ggattcacta ccgctggcgg atcgcgccac cattgccaat
     2941 atgtcgccag aatatggtgc cacctgtggc ttcttcccaa tcgatgctgt caccctcgat
     3001 tacatgcgtt taagcgggcg cagcgaagat caggtcgagt tggtcgaaaa atatgccaaa
     3061 gcgcagggca tgtggcgtaa cccgggcgat gaaccaattt ttaccagtac gttagaactg
     3121 gatatgaatg acgttgaagc gagcctggca gggcctaaac gcccacagga tcgcgttgca
     3181 ctgcccgatg taccaaaagc atttgccgcc agtaacgaac tggaagtgaa tgccacgcat
     3241 aaagatcgcc agccggtcga ttatgttatg aacggacatc agtatcagtt acctgatggc
     3301 gcggtggtca ttgctgcgat aacctcgtgc accaacacct ctaacccaag tgtgctgatg
     3361 gccgcaggct tgctggcgaa aaaagctgta actctgggcc tcaagcggca accgtgggtc
     3421 aaagcgtcgc tggcaccagg ctcgaaagtt gtttctgact atctggcaaa agcgaaattg
     3481 acaccgtatc tcgacgaact ggggtttaac cttgtggggt acggttgtac cacctgtatt
     3541 ggtaactctg ggccgctgcc cgatcctatc gaaacggcaa tcaaaaaagg cgatttaacc
     3601 gtcggtgcgg tgctgtccgg caaccgtaac tttgaaggcc gtatccatcc gctggttaaa
     3661 actaactggc tggcctcgcc gccgctggtg gttgcctatg cgctggcggg aaatatgaat
     3721 attaacctcg cttctgagcc tattggtcac gatcgtaaag gcgacccggt atatctgaaa
     3781 gatatctggc cctcggcaca agaaattgcc cgtgcggtag aacaagtctc cacagaaatg
     3841 ttccgcaaag agtacgcaga agtttttgaa ggcacagcag agtggaagga aattaacgtc
     3901 acacgatccg atacctacgg ttggcaggaa gattcaacct atattcgctt atcgccattc
     3961 tttgatgaaa tgcaggcaac accagcacct gtggaagata ttcacggtgc gcggatcctc
     4021 gcaatgctgg gggattcagt caccactgac catatctctc cggcgggcag tattaagccc
     4081 gacagcccgg cgggtcgata tctacaaggt cggggtgttg agcgaaaaga ctttaactcc
     4141 tacggttcgc ggcgtggtaa ccatgaagtg atgatgcgcg gcaccttcgc caatattcgc
     4201 atccgtaatg aaatggtgcc tggcgttgaa ggggggatga cgcggcattt acctgacagc
     4261 gatgatgtct ctatctatga tgccgcgatg cgctataagc aggagcaaac gccgctggcg
     4321 gtgattgccg ggaaagagta tggatcaggc tccagccgtg actgggcggc aaaaggtccg
     4381 cgtctgcttg gtattcgtgt ggtgattgcc gaatcgtttg aacgaattca ccgttcgaat
     4441 ttaattggca tgggcatcct gccgctggaa tttccgcaag gcgtaacgcg taaaacgtta
     4501 gggctaaccg gggaagagaa gattgatatt ggcgatctgc aaaacctaca acccggcgcg
     4561 acggttccgg tgacgcttac gcgcgcggat ggtagccagg aagtcgtacc ctgccgttgt
     4621 cgtatcgaca ccgcgacgga gttgacctac taccagaacg acggcatttt gcattatgtc
     4681 attcgtaata tgttgaagta acaagtattt gcttgccggt tattttgctt ccggcaagca
     4741 aatgaattac acaatgcaag agggttattt gttcagcaaa tggcccattt tctcggcttt
     4801 ggtatcgaga taatgttcgt tattggggtt acgacctaca atcaatggta cgcgttcaac
     4861 aatattaatc cctgcttcgg tcagaatttc gacttttttc gggttattgg ttaacaagcg
     4921 gacttcattg acgccaagga gtttgaacat atcagcgcaa agagtgaagt cgcgctcatc
     4981 agcggcgaag cctaactggt ggttagcctc tacagtatcg taaccttgat cctgcagtgc
     5041 ataagcgcgg attttattca gcagaccaat gttacgacct tcctgacggt gatacagcaa
     5101 aataccacgg ccttcttcgg caatttgcgt cagtgccgct tcgagctgga agccacaatc
     5161 gcagcgcaag ctgaacaggg cgtcaccggt cagacattcg gaatggacgc gcgcaagtac
     5221 cggggtatgc ccggagatat cgccatagac tagcgcgaca tgatcgtgtc cggttgccag
     5281 ttcttcaaat cccaccatca ggaaatcgcc ccatggggtt ggcagtttgg cttctgccac
     5341 acgtttaagc tgcatgaaat tctccagata atgctggttc tgtattggct tattttgcca
     5401 taacgagaag gggttcacct aatcacgacg cgtcgatcgt tcacggaatg gcacaaatct
     5461 gtcaattttt cctggaactg gcgttttcag ttatgattgt gggacttatc aaaaaggaga
     5521 ggccatgcgt tcgattgcca gacgtaccgc agtgggagct gcactattgc ttgtcatgcc
     5581 agtagccgta tggatttctg gttggcgttg gcaacctgga gaacaaagtt ggctactaaa
     5641 agcggctttt tgggttactg aaactgtcac ccagccctgg ggcgtcatta cacatttgat
     5701 tttattcggc tggtttctct ggtgtctgcg ttttcgcatt aaggctgcct ttgtattatt
     5761 tgccattctg gcggcggcaa ttcttgtggg acaaggcgtt aaatcctgga tcaaagacaa
     5821 agtccaggaa ccacgacctt ttgttatctg gctggaaaaa acacatcata ttccggttga
     5881 tgagttctac actttaaagc gagcagaacg cggaaatctg gtgaaagaac agttggctga
     5941 agagaaaaat atcccacaat atttgcgttc acactggcag aaagagacgg ggtttgcctt
     6001 tccttccggt cacacgatgt ttgctgccag ttgggcgctg ctggccgttg gtttgctgtg
     6061 gccgcgtcgg cgaacgttaa ccattgctat cttgttggtc tgggcaacgg gagtcatggg
     6121 aagccgcctg ctgctcggga tgcattggcc acgcgatctg gtggtagcta cgttgatttc
     6181 gtgggcgctg gtggcggtgg caacgtggct tgcgcaacga atttgtgggc cattaacacc
     6241 acctgcggaa gaaaatcgcg aaatagcgca acgagaacaa gaaagttaac gctggttgat
     6301 tttccgaatt tagcccttaa atcatcatca atgcgcgtgg atgccatttc gcagacggcg
     6361 cgaaaatggt actttaaagg gctattgcgg taagttgacc ataatttatt cgctctaacc
     6421 acataacggg aagtaatgtg aaatatttac tcattttctt actggtgtta gcgatcttcg
     6481 tgatttcggt cacgttaggt gcgcagaacg atcaacaggt gacgtttaat tatctgttag
     6541 cgcaagggga gtaccgtatt tccacattgc tggcggtatt gtttgctgcg gggtttgcta
     6601 tcggttggct gatttgcggt ctgttctggc tgcgagttcg tgtttccctg gcgcgcgctg
     6661 aacgtaaaat aaagcgactg gaaaaccagc tttcacccgc gactgacgtg gctgtagcgc
     6721 cgcattcgtc agcggcgaag gaataacttt ctatgctgga gttgttgttt ctgcttttgc
     6781 ctgtagccgc tgcctatggc tggtatatgg gccgcagaag tgcgcaacaa aacaagcaag
     6841 atgaagccaa ccgcttgtcg cgtgattacg tagcgggggt taacttcctg cttagtaatc
     6901 aacaggataa agcggtagac ctgtttctcg atatgcttaa agaggatacg ggtaccgttg
     6961 aagcccacct tacgctagga aacctgttcc gttcgcgtgg cgaagttgat cgcgctattc
     7021 gcatccatca gaccctaatg gaaagcgcct cgctgaccta tgaacagcgt ctgttggcga
     7081 ttcaacaact ggggcgtgat tacatggccg ccgggttata cgaccgcgcg gaagacatgt
     7141 tcaatcagct gaccgatgaa actgacttcc gcattggcgc gctgcaacag ttgctacaaa
     7201 tctaccaggc taccagcgag tggcagaaag caattgatgt tgccgaacgt ctggtgaagt
     7261 tgggtaaaga taagcagcgc gtcgaaattg cccatttcta ctgtgagtta gccctgcagc
     7321 atatggccag cgacgatctc gatcgcgcca tggccttgtt aaaaaaaggt gcggcggcag
     7381 ataaaaacag cgcccgcgta tccatcatga tgggacgcgt atttatggcg aaaggagaat
     7441 acgccaaagc cgtcgaaagt ctgcaacgcg tgatatctca ggacagagaa ctggtcagcg
     7501 aaacgctgga aatgttgcaa acctgctatc agcagttggg taaaactgcc gaatgggcag
     7561 aattcctgca gcgcgcggtg gaagagaaca ccggtgccga tgctgaattg atgcttgcgg
     7621 atattatcga agcgcgcgac ggtagtgagg ccgcacaggt ctatattacg cgtcagcttc
     7681 agcgtcatcc gaccatgcgt gtgttccata agctaatgga ttaccactta aatgaagcgg
     7741 aagaagggcg tgccaaagag agtctgatgg tgctgcgtga catggttggc gagaaggtgc
     7801 gtagtaagcc tcgttatcgc tgccagaaat gtggttttac cgcatacacc ctctactggc
     7861 attgtccgtc ttgtcgggcc tggtcaacca ttaaaccgat tcgcggtctt gatggcctgt
     7921 aatttttaaa aaaatccgac tttagttaca acatactaat aattgatgtt cattgtgctc
     7981 cggtaactcc ggcgcagaaa agcctgccag gggagaaatc gcaactgtta attttttatt
     8041 tccacgggta gaatgctcgc cgtttacctg tttcgcgcca cttccggtgc ccatcatcaa
     8101 gaaggtctgg tcatgacgtt aactgcttca tcttcttccc gcgctgttac gaattctcct
     8161 gtggttgttg cccttgatta tcataatcgt gatgccgcgc tggcctttgt cgacaagatt
     8221 gacccacgcg attgtcgtct gaaagtcggc aaagagatgt ttacgctgtt tggacctcaa
     8281 tttgtacgcg aacttcaaca gcgtggtttt gatatcttcc tcgacctgaa atttcacgat
     8341 attcccaaca ctgcagcgca cgctgtcgct gctgcagctg acttaggcgt gtggatggtg
     8401 aatgttcacg cctccggtgg ggcgcgtatg atgaccgcag cgcgtgaggc actggtgccg
     8461 tttggcaaag atgcaccgct tttgattgct gtgacagtat tgaccagcat ggaagccagc
     8521 gacctggccg atcttggcgt gacactgtca cctgcagatt atgcagaacg tctggcggca
     8581 ctgacgcaaa aatgtggcct tgatggtgtg gtgtgttctg ctcaggaagc tgcgcgcttt
     8641 aaacaggtat ttggtcagga gttcaaactg gttacgccgg gcattcgtcc gcaggggagt
     8701 gaagctggtg accagcgccg cattatgacg ccagaacagg cgttggcggc tggtgttgat
     8761 tatatggtga ttggtcgccc ggtaacgcaa tcggtagatc cagcgcaaac gctgaaagcg
     8821 atcaacgcct ctttacagcg gagtgcatga tgagtgattc caacagccgt ctggtctact
     8881 caacggaaac cggacgtatt gatgagccaa aagcggcccc tgtacgccct aaaggcgacg
     8941 gtgtggtgcg tattcagcgt cagaccagtg gacgtaaagg taagggcgtt tgcctgatta
     9001 ccggtgtcga tctcgatgat gccgaactga caaaactggc agcggaactg aagaaaaaat
     9061 gcggctgcgg cggggcagtt aaagatggaa ttattgaaat ccagggcgat aagcgtgatt
     9121 tattaaaatc actgcttgaa gcgaaaggga tgaaggtaaa actcgccggt ggttaacata
     9181 aaaagccacg gatatatccg tggctttcga atattttact gtgtgtatta ttcagttatt
     9241 tcgtactgaa cagaatcaag cgtattataa ttgcgagtcg ataatcgaca tttatttacg
     9301 attatttacc gacctggtga ccaataacac caccgacagc tgcgccacct aatgtaccca
     9361 acgtactgcc atcggtcagt actgcaccgc ctaatgcccc tgcacccgcg ccgattgcgg
     9421 tgttgcgatc ccgtttagac cagttagaac aggcactcag agacattgcc agggtaattg
     9481 ccagaacagc cgcggtcatt tttttgctcg ttacaaacat aatactctct cctgaattta
     9541 tgattcacgg aagtaagctc tctataacta taatagctaa gaataagcct ggtgaaatta
     9601 ttccgtgaaa tccgctc
//
LOCUS       DAZGAP010000183         9558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGAP010000183 DAZGAP010000000
VERSION     DAZGAP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.184"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2932
                     /gene="evgS"
                     /locus_tag="ACHLAM_004240"
     CDS             <1..2932
                     /gene="evgS"
                     /locus_tag="ACHLAM_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO7855688.1"
                     /translation="RYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTN
                     EVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGS
                     VRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSF
                     AEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASA
                     AFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKD
                     IINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGF
                     YLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADY
                     YKNAMLPLENSESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLC
                     NLPASEHAVYICGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISS
                     IMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVD
                     IPTLVQNTCHSFGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTT
                     EGAVKITTSLGHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLG
                     LMICKELIKNMQGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSI
                     LIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFE
                     LTRKLREQNSSLPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVA
                     HIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQC
                     IHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFC
                     QQND"
     gene            complement(2988..4133)
                     /gene="yfdE"
                     /locus_tag="ACHLAM_004241"
     CDS             complement(2988..4133)
                     /gene="yfdE"
                     /locus_tag="ACHLAM_004241"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO7855689.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4207..5151)
                     /gene="yfdV"
                     /locus_tag="ACHLAM_004242"
     CDS             complement(4207..5151)
                     /gene="yfdV"
                     /locus_tag="ACHLAM_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO7855690.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5221..6915)
                     /gene="oxc"
                     /locus_tag="ACHLAM_004243"
     CDS             complement(5221..6915)
                     /gene="oxc"
                     /locus_tag="ACHLAM_004243"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO7855691.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6969..8219)
                     /gene="frc"
                     /locus_tag="ACHLAM_004244"
     CDS             complement(6969..8219)
                     /gene="frc"
                     /locus_tag="ACHLAM_004244"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO7855692.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(8732..9364)
                     /locus_tag="ACHLAM_004245"
     CDS             complement(8732..9364)
                     /locus_tag="ACHLAM_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO7855693.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         2748 a         2208 c         1986 g         2616 t
ORIGIN      
        1 ccgttatttt actcactcct taaatgtagt gaagtattat aactcaccgc gtcaatataa
       61 ctttttatta accagaaaag attctatcgt tcttaatgaa gtactcaatc gatttgttga
      121 tgctttaaca aatgaagttc gctatgaagt atcacaaaat tggcttgata caggaaatct
      181 ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga ttaagcagca
      241 tcccgattta aaggtgctag aaaatcctta ctcgccaccc tattctatga cagatgaaac
      301 tggctcagtt cggggcgtga tgggtgatat tcttaatatt attaccttgc aaacaggttt
      361 aaatttttct ccgatcaccg tttcacacaa tattcatgct gggacacagc ttaaccccgg
      421 tggatgggat atattgcccg ccgctattta tagtgaagat cgagaaaata atgtttcatt
      481 tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg caaaaagcgc ctgacagtga
      541 acaaacatta aaaaaaggaa tgaaagttgc cattccctat tattatgagc ttcattcgca
      601 attaaaagag atgtatccgg aggtagagtg gataaaagtc gataacgcca gcgctgcatt
      661 tcacaaggtt aaagaaggtg aacttgatgc tctggtcgcg acacagttaa attcgcgtta
      721 catgatcgac cattactatc ctaatgaact ttatcatttt cttattcccg gcgttcagaa
      781 tgcatcactt tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata ttattaataa
      841 agcactgaat gcaattcccc caagcgaagt tctgcgcctg accgaaaaat ggattaaaat
      901 gcccaatgtg accattgaca catgggacct ctatagcgag caattttata ttgttacgac
      961 attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt tacgctcagt
     1021 tcgtcgtcgt aaagttattc agggtgattt agaaaaccaa atatcattcc ggaaagcgct
     1081 ctcggattcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg tcattagtca
     1141 caatagtgca tttgaacatt atttcactgc tgattactat aaaaatgcaa tgttaccatt
     1201 agaaaacagt gaatccccct ttaaagatgt tttttctaat acgcatgaag tcacagcaga
     1261 aacgaaagaa aaccgaacaa tatacacaca ggtatttgaa attgataatg gcatcgagaa
     1321 aagatgcatt aatcactggc ataccttatg taatctgcca gcaagcgaac atgctgttta
     1381 tatttgtggt tggcaagata ttaccgagac gcgtgattta attcatgcac tcgaagtaga
     1441 gagaaataaa gcgatcaatg caactgtcgc aaaaagccag tttctggcaa caatgagtca
     1501 cgaaataaga acaccaataa gctccattat gggcttcctg gaacttctgt cgggttctgg
     1561 tcttagcaag gaacaacggg tggaggcgat ttcacttgcc tacgccaccg gacaatcact
     1621 cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta actatcaact
     1681 tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact ctttcggtgc
     1741 gattgctgca agcaaatcga tcgcattaag ttgcagcagt acgcttcctg aacgctacct
     1801 ggttaagatc gaccctcagg cgtttaagca ggtcttatct aatttactga gtaatgctct
     1861 caaatttact accgaggggg cagtaaaaat tacgacctcc ctgggtcaca ttgatgacaa
     1921 ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc aggaagaaca
     1981 acaacaactg tttaaacgct atagccaaac aagtgcaggt cgtcagcaaa caggttctgg
     2041 tttaggctta atgatctgca aagaattaat taaaaacatg cagggcgatt tgtcattaga
     2101 aagtcatcca ggcataggaa cgatatttac gatcacaatc ccggtagaaa ttacccaaca
     2161 agtggcggct gtcgaggcaa aagcagaaca acctataaca ctacctgaaa agttgagcat
     2221 attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt
     2281 aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca
     2341 acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac
     2401 tcgcaaactc cgagagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca
     2461 ggctaacgaa cgtgaaaaag ggttaaattg cggcatgaac ttatgtttgt tcaaaccgtt
     2521 gactctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc
     2581 tcagtatcgc caccttgata tcgaggccct gaagaataat acggcgaatg atctacaact
     2641 gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac ccgctgcgtt
     2701 tcatgcacta gaagctggcg ataacagaac tttccatcag tgtattcatc gcatccacgg
     2761 tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccaccagt tagaaataac
     2821 acccgtttca gatgacagta agcctgaaat tcttcagttg ctgaactctg taaaagaaca
     2881 cattgcagag ctggaccagg agattactgt tttctgtcag caaaatgact aaatagcggc
     2941 tcccacaatg ttcaaatgtg ggagctattt accagcacat cttacattta tgatgagaac
     3001 tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcggg
     3061 tccgcgcagc cgctgatttt tatcggattc cccggcatca ttattccccc ggcttcaatc
     3121 aacatatttc tcgcctgagt ttgtggcaaa ttaatggcct cagccacact taataacggc
     3181 gcgacgggta caccaacttc atgtattctg gctaaccaaa cttcagctgc ctgcgttttt
     3241 aacgtccgct caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg
     3301 ctaaatctgg gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa
     3361 agcttgtcat taccacaaca aatcgtaatc ggcttatcct gagtatcgaa aacatcaaaa
     3421 ggtgccatgt agggatggcg atttcccagg cgttgtggtg acttccctgt cgcaatatat
     3481 gccatcagac catgctccag aaaactcagc gtggcatcaa acatcgctat atcgacatgc
     3541 gcccctcttt ggctcttttc gcggccataa agtgcactca ctattccgct gaataaatag
     3601 acaccgccgc atagatccgc aagagaggta cccacgcgca ctggcggagc atcagggtat
     3661 cctgtttcca tcattatccc gctcattgcc tgaatgatgg tatcgtaggc aggagcatct
     3721 tttagcggac cggtatgtcc gaaacctgac gatgaagcat atataaggcg cgggttgatt
     3781 tcttgtaacc tttcccatga aaacccgagt ttttccattg tacctgggcg aaaattctca
     3841 gctaatacat ccgcttgttt aagcatattt ataaatatac ttttatcgtg atcattcttt
     3901 aaatcaagaa ccacactctc tttgccatga ttaataaaac tgtaatagag tgactgtcca
     3961 tccacatagg gaccaaatgt gcgggtatca tcaccatgac ccggcggctc aactttaatt
     4021 acccttgcgc ccatattaca aagaagttga gttccgaaag gcccattaag gacatgcgtc
     4081 atatcgataa ctaataagcc ttcaaacggc cctttgcatt cattatttgt catttgctta
     4141 tgccttgaaa aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataacat
     4201 gattaattat gaaaccagtc gactgacata aatccacaaa ggagccgtga cgacaaaacc
     4261 cagtacgctc acagccaatg acgctgtacc agtgcgggtg tagacattaa accggctggc
     4321 aataatgatt ccggagaatg ccggcggtaa ggcgcctgcc agtaccatca tctgcaaatg
     4381 ttcgctgttc aaatgacatg ccataccaac gagaagcagc gccagtggca tcagaatcag
     4441 cttcaggaag gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt
     4501 caaccccgca gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggttgg
     4561 gtcccatgct gccggaattt ttaccccaac caacaccagg atcgttgcca gaacaggtgc
     4621 ccatactact ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc
     4681 atccgctcct gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcattaac
     4741 aataatagaa ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc
     4801 gagaactgca aatcccagga atccaatggt aggtgaacct gcaattaatg cacatactgc
     4861 tgcttctgca tgagtacgtt taaaaaattt gtagcaaccg aaccaggaga aaaagaaaca
     4921 tccgacaata accacaagtg atacaagggt cagacgagtg tccgcaaaaa tcatttccct
     4981 gtttgcccgg gtaatagata caaatagagc tgcaggaaga gcgtagttta ataccagttt
     5041 attaaatgcc cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa aataacccaa
     5101 cagcatgatt acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt
     5161 tccgataagg gtcccctcac catcgcataa tgagttggca agaggagacg atgtcagtat
     5221 ttaattacca gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc
     5281 tgcagggtcg atgaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa
     5341 agcatgacga agttcatctg tcgtagtgac gttatagcca acgccacgaa acgcatccat
     5401 taatttgtca taccttgcat ggtgcagcag atccgttggt gatggtgcac cagcgccgct
     5461 gagatcaaca ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg
     5521 caggttatat cgacaaatcg tttcaatttc catcccactg aaaccaaaag cactatcacc
     5581 ttcaatggca acaaccggag aaccagaggt cacgctagca ccgatggcat agcccatacc
     5641 gatgcccatg acaccccagg tgccacaatc cagacgacga cgtggtttat acatatcaat
     5701 aatatttcgt gcattatcca gggtatttgc cccttcatta actaaataaa tatcctggtt
     5761 ctcgcgcaat acgtcgcgca cagcacttaa tgcattaaag taatttaatg gttgagtatc
     5821 tgtgcttaac ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc
     5881 gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt tctgccagca taccttgcat
     5941 actggatgca atatcgccaa cgactggcac agcaatgggg cggttgctgt caatttcctg
     6001 cggttcaata tccagttgaa taaactgtgt atctgccgcc catccttttt taccgtgtgc
     6061 cagtaaccaa ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa
     6121 cgaacgcgca gccgccgcag aaagtggatg cgtatcttcg aggatccctt tcgccataga
     6181 cattggcagg aatggaatct gggcactttc aataaattca cgaagctgtt catccgcttg
     6241 tgaatacgcc gcgcctttgc caaggataac taatggtcgt tcagcttttg ctaatagcga
     6301 aattgcgcta gcgactgact tcgggcatgg caataatgct ggcgacggat tttcaacttt
     6361 tacaatcgtg gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgctggcaa
     6421 atcaagataa actccgccag ggcgacccga tactgagacc cggatagcgc gtgccaatgc
     6481 aatgccaaga tcctgcggct gattaacgcg gaatgctgct ttggcatacg gttttgccgc
     6541 attcatttgg tccagctctt cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct
     6601 ggagccgcta atcatgatca tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa
     6661 accattgagg aatcctggcg cagaaactgt caggcagatc cccggttttt gggtaagaaa
     6721 accgcttgcc gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat
     6781 gccttccgcc tgggcatggc gcgccatatc cgtcacagga atacctacaa caccataaat
     6841 agtgtcaata ccattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg
     6901 aagttgatct gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc
     6961 cgttaatatc agatggcgtg gttttgcttc attgcagcaa tttcatcgtc gctataaccc
     7021 agctcctgca atacagcagc ggtatgctca cctaatagcg gcgcagcttt aatatccggc
     7081 gtaaaggcag agaatttcat tggacaacca acggtcagat attttccacg caacggttgt
     7141 tccacttcga caacactacc actttggcgc aaagagggat caagtgaaat ttctttcata
     7201 cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc ttcatgttta
     7261 tcaatagtga cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgg
     7321 gcatgggctg tactgtatgc cggatcggta atccattctg gtttgccgat ggctttacag
     7381 gtgttttccc agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc
     7441 cagcctttac atttcaggat ccagccaggc tgaccgccac cacccgcatt accaccgcgg
     7501 ggaactgcat caccaaatgt gccattcgga tactgcgggt attcttccag ataccccaac
     7561 ttatcgagac gctgctggtc gcgtaatttc acgcggcaaa ggttcaacac ggcatcctgc
     7621 attgacatgg tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt
     7681 aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc
     7741 ggcggaccgt cccaaaaacc tgtagtagat gccgcgccac ccgctgcctg agcaacgttt
     7801 tcataggctt ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa
     7861 atcagacgtg gattgatttc ttgaatatgc tcccaggtga agcccatatg atcaatggcc
     7921 cctggatgaa agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct
     7981 ttgccttccg ctgttttggt atttaattca atagaacgtt tgttactgtt aagcatggtg
     8041 aagtaaagcg catcgatatc aggaatatct cgcagctggt ggcgcgttac gtcaccaacg
     8101 ccgggacgtt caattttaat gacgtcagcg ccaaaccagg ccagcatctg agtacaagat
     8161 gggccagatt gcacaccagt gaaatcgaga actttaattc cttgaagtgg agttgacata
     8221 gcaataccta ttagttaatt aaatatatgt taatttagcg tttatttaag gcataatgct
     8281 ctctataaat aatccgcaaa atatcgtcgt agctgcactc gatattttta aattcctgta
     8341 tcaagtgctg ataatcattt catcgccact tccaattcct ttctgcatgg taagtagtga
     8401 ggacgaaaag aagaatatga taaatattgg ttcgcggtaa tcagagaggg ccgcgacatt
     8461 tgtgatgtta ttcttacaac tgtaagaagc gccggtgatg tacaagtaag ggtatgatta
     8521 cgctttttaa agcgtaccat aaaacataac ataataaatg atttatgatg acacgggatt
     8581 attgcggttt actttgaaga atataaaatg cgagtttata gcaccgcgag cgccaaataa
     8641 ttttatggcc acaacctgtc gtattgtggc cataaaaaat tgtcggctgg agggagtaaa
     8701 atcctttacc tcaatatatt gcctgtcagc tttagttcac aaacagtgct tcgctatcga
     8761 cgatgattcc atcttccgca cctttaagcg ccaggtttgc ctcgtagtat tgctttttat
     8821 caagcagttt ctgcgcatca gcaagtgcat tacgagtctg tttgagcggc atcagatatt
     8881 ggttttccat tacaccaaca ccggccaggc gaagctcttc cattgcgccc tttttatccc
     8941 ctttcgccat tttttcgttg gcaattttta tcgcggcttc tttttcgggt gtcgctacat
     9001 aactctcaga aattccgaca gatgcattaa tgacaatata ttggtcatca ttgagattgg
     9061 tctttttacc tggcttagcg aactttgccc attcggtact gtcatctgac aacaaagctg
     9121 aagcttcatt ggtcagttct tttgcctttt ccggatcgcc atgaaacaga gccaggcgcg
     9181 ctacctgcac gtcacgcatc gcatataaac cttgttcaga gatacgctct gctgctgccg
     9241 ttttctgggt ttgctgaact tgctgagttt gctgttgtgc cgctaccggt gcattatcag
     9301 cagcccatac agttgaagat gccagaattg ctgtgaccat cgtagccata attaaacgtt
     9361 tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt gggtgagcat
     9421 gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacag ttgtagttat
     9481 tatcgcaaga agtattttag tcttacaatt gtaggaatat gcttcctgtc acagagaact
     9541 attcttatat accacaaa
//
LOCUS       DAZGAP010000184         9546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGAP010000184 DAZGAP010000000
VERSION     DAZGAP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.185"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            110..721
                     /gene="ruvA"
                     /locus_tag="ACHLAM_004246"
     CDS             110..721
                     /gene="ruvA"
                     /locus_tag="ACHLAM_004246"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO7855694.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            730..1740
                     /gene="ruvB"
                     /locus_tag="ACHLAM_004247"
     CDS             730..1740
                     /gene="ruvB"
                     /locus_tag="ACHLAM_004247"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO7855695.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(1890..2675)
                     /gene="znuB"
                     /locus_tag="ACHLAM_004248"
     CDS             complement(1890..2675)
                     /gene="znuB"
                     /locus_tag="ACHLAM_004248"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO7855696.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAIMFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2672..3427)
                     /gene="znuC"
                     /locus_tag="ACHLAM_004249"
     CDS             complement(2672..3427)
                     /gene="znuC"
                     /locus_tag="ACHLAM_004249"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO7855697.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRHELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3506..4438
                     /gene="znuA"
                     /locus_tag="ACHLAM_004250"
     CDS             3506..4438
                     /gene="znuA"
                     /locus_tag="ACHLAM_004250"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO7855698.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            4454..5776
                     /gene="mepM"
                     /locus_tag="ACHLAM_004251"
     CDS             4454..5776
                     /gene="mepM"
                     /locus_tag="ACHLAM_004251"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO7855699.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5896..6867
                     /gene="lpxM"
                     /locus_tag="ACHLAM_004252"
                     /gene_synonym="msbB"
     CDS             5896..6867
                     /gene="lpxM"
                     /locus_tag="ACHLAM_004252"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7855700.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6999..8441)
                     /gene="pyk"
                     /locus_tag="ACHLAM_004253"
     CDS             complement(6999..8441)
                     /gene="pyk"
                     /locus_tag="ACHLAM_004253"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO7855701.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8569..9438)
                     /gene="hexR"
                     /locus_tag="ACHLAM_004254"
                     /gene_synonym="yebK"
     CDS             complement(8569..9438)
                     /gene="hexR"
                     /locus_tag="ACHLAM_004254"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO7855702.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAIVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
BASE COUNT         2438 a         2397 c         2452 g         2259 t
ORIGIN      
        1 ttcagatcat cgggacacct cgcaagtttt cttcatcctt cgctggatat ctatccagca
       61 tttttttatc atacagcatt atctttgatt cattacgcag gagcgtcatg tgataggcag
      121 actcagaggc atcatcattg aaaaacaacc cccgctggtg ttaattgaag tgggcggcgt
      181 aggctatgaa gtgcatatgc cgatgacctg tttttatgaa ctccctgaag cgggtcagga
      241 agcgatcgtt ttcacccact ttgtggtgcg tgaagacgcg caactgctgt acggttttaa
      301 caataaacaa gagcgcacgt tgttcaaaga gttgatcaaa accaacggcg tcggcccgaa
      361 gttggcgctg gcgatcctct ccggaatgtc agcgcagcag ttcgttaatg ccgttgagcg
      421 tgaagaagtg ggggcactgg tgaaactgcc gggtattggc aaaaaaaccg ccgaacgctt
      481 gattgtcgaa atgaaagacc gatttaaagg tttgcatggc gatctcttta cgccagccgc
      541 cgacctggta ctcacgtcac ctgccagccc ggcgaccgac gatgctgaac aggaagcggt
      601 tgctgcgctg gtggcgctgg gctataaacc acaagaagca agccgtatgg tgagcaaaat
      661 cgctcgccct gacgccagca gtgaaacatt aattcgcgaa gccctacgcg ccgcgttatg
      721 aggtaaagga tgattgaagc agaccgtctg atttctgccg gtaccacttt gccggaagat
      781 gtggcagatc gcgccattcg ccctaaatta ctggaagagt atgttggtca accgcaggtt
      841 cgttcacaga tggagatttt catcaaagca gcgaaactgc gcggcgatgc cctcgatcat
      901 ctgttgattt ttggtcctcc ggggttgggt aaaactacgc ttgccaatat tgtcgccaat
      961 gaaatgggcg ttaatttacg cacgacttct ggtccggtgc tggaaaaggc gggcgatctg
     1021 gcagcaatgc tcactaacct tgaaccacac gatgtactgt ttattgatga gatccaccgt
     1081 ctttcaccag tggtggaaga ggtgttgtat ccggcaatgg aagactacca actggatatc
     1141 atgattggtg aaggtccggc ggcacgctcc attaaaattg atttgccgcc gtttacactg
     1201 attggcgcaa ccacgcgcgc aggttcgctg acatcaccgt tgcgcgatcg ttttggtatt
     1261 gtgcagcgtc tggagtttta tcaggtgccg gatctgcaat atatcgtcag tcgcagcgca
     1321 cgctttatgg ggcttgagat gagtgatgac ggcgcgctgg aagttgctcg tcgcgctcgc
     1381 ggtacgccgc gcattgctaa ccgtctgctg cgtcgagtgc gtgatttcgc cgaagtgaag
     1441 cacgatggca ccatctcggc tgatatcgct gctcaggcgc tggatatgct gaatgtcgat
     1501 gccgaaggtt tcgattatat ggaccgcaaa ttgttgctgg cggtaatcga taagttcttt
     1561 ggtgggccgg taggtctgga taacctggcg gctgccattg gcgaagaacg tgaaaccatt
     1621 gaggatgtgc tggaacctta tttgattcag caaggctttt tgcagcgtac accgcgcggg
     1681 cgtatggcga cagtgcgggc gtggaaccac ttcggcatca cgccgccaga aatgccgtaa
     1741 gtcggatcgg tcgctcgcgg cgcatccgac actggcagtg ttgcctgatg cgccgctatt
     1801 cgcgtcttat caggcatata ccagagatag cgaaccatag gtcggataag gcgctcgcgc
     1861 cgcatccgac aaatgtgttc agcgatagat tagctggcct gctttttcat catactgata
     1921 ataaataaca gtgccgcaca tagcaccacc gacgggcctg caggtgtatc gtaaaatgcg
     1981 gaaaaggtta aaccgccagt cactgccacc atccccacca aaacagcgac accagccatc
     2041 tgttccggcg tgcgggcaaa gcgacgtgca gtagcagcag gaataatcag cagtgaagta
     2101 ataatcaacg cgccgacaaa tttcatcgct acaccaatcg tcaatgccgt caccagcatc
     2161 aacaacaatt tcacgcgctg taatttcaca ccatcaacaa acgccagatc cgggctaatc
     2221 gtcatcgaca gcaaattgcg ccattgccag aacataatag ccaccacgat gaccacgcca
     2281 atcgcaatag agatgagatc ttctggcgtc actgccagta aatcaccgaa caggtaagcc
     2341 atcaaatcaa cacgaatatt agacatcaga ctaacgacca ccaggcccag cgacagggca
     2401 ctgtgcgcca taatccctaa taacgtgtcg atcgccagct gtggacgctt ctccagccat
     2461 accagaccgc ccgccagcag cagcgtaact gcaatcaccg catagaatgg attcacgtcc
     2521 agcaacaaac caaacgcgac gccaagtaat gaggcatgag ccagcgtatc accgaaataa
     2581 gacatacgac gccagactac aaacgaaccc agcggacccg cggcacaggc gagcatgatc
     2641 ccggctaacc aaccgggaaa taataattca atcatgagcg atcatttccc cgacgcaaaa
     2701 caattcgtcc ctgtaaatcg tgacgatgat tatgatgatg gcgatagata cccagttgtt
     2761 cagcaccacg aggaccaaac atagaaataa actccggatg cagggaaaca acttccggtg
     2821 tgccggaaca acaaatgtgg tgattcaggc aaagcacttc atcggttttt gccattacca
     2881 gatgcagatc gtgagatacc attaaaacgc cacaatccag ttcgtgacgc agttggtcaa
     2941 taaggtcata taacgccacc tgaccattca catccacgcc ctgagtgggt tcatccagca
     3001 ctaataattg cggtcgattt aacaatgctc gcgctaacag tacacgctgc gtttcgccac
     3061 ccgagagctt ttgcatcggt gcgttaatca gatgcccggc ctggacacgt ttcagtgcag
     3121 gcaaaatatc ttctttatgt gtgccagggc gtaagcgtaa aaaacggttt acggtcagtg
     3181 gcaacgtggt gtcgagatac agcttctgcg gtacatagcc gatgcgcagt tttccgttgc
     3241 gcttgataac cccttcatcg ggtgttacca gcccgagcac tacccgtacc agtgtcgact
     3301 tacctgcgcc gtttggccca agtaaagtca aaatttttcc aggtttaagt tccagcgaca
     3361 catcagagag gacgcggcgt tggccaaaag aaaccgagac attttccagg gaaaccagac
     3421 ttgtcatgtt aattttagtc ttgcagtagt catgaaatgt tataatatca cagttctcat
     3481 attcattacg atgattggtc gcattatgtt acataaaaaa acgcttcttt tcgcagcatt
     3541 atccgccgct ctctggggcg gtgcaacaca ggccgcagat gccgccgttg tcgcttcgct
     3601 taaacccgtt gggttcatcg cttctgccat tgctgatggg gtaacagaaa cgcaggtttt
     3661 actgcctgac ggcgcttcag aacatgatta ttcactgcgt ccatcggatg taaaacgctt
     3721 acagaacgcg gacttagtcg tttgggttgg cccggagatg gaagcgttca tgcaaaaacc
     3781 ggtaagtaaa ttaccagaag cgaaacaggt aacgattgcg cagctagaag atgtgaaacc
     3841 gctgctgatg aaaagtattc acgacgatga tgatgatcac gaccacgcgg aaaaaagtga
     3901 cgaagatcac catcacggcg atttcaacat gcatctttgg ctttccccag agatagcgcg
     3961 ggctacagcg gttgcaatcc atggaaaatt agtggaactt atgccgcaaa gtcgagccaa
     4021 acttgacgcc aacctgaagg attttgaggc acaattagcc tcaaccgaaa cgcaggttgg
     4081 taacgagctc gcgccgctca aggggaaagg ttatttcgtt tttcacgatg cttacggcta
     4141 cttcgaaaaa cagttcggac tgacaccgct tggtcatttt accgttaacc ctgagattca
     4201 acctggcgcg cagcgtttac atgaaataag aacacagttg gttgagcaaa aagcaacctg
     4261 cgtttttgct gagccacagt tcaggccagc ggtcgttgag agcgtcgcac gagggacatc
     4321 cgttcgtatg ggaacgttgg atcccctggg gacgaatatc aaactgggta aaacaagcta
     4381 ttcagaattc ctgaatcaat tagccaacca gtatgcgagc tgcctgaaag gagattaatg
     4441 aggaagtgat tacgtgcaac agatagcccg ctctgtcgcc ctggcgttta ataatttacc
     4501 gcgaccacac cgcgttatgt tagggtcgct caccgttctt actctggccg tcgctgtctg
     4561 gcggccctat gtttatcacc gcgatgccac gccaattgtc aaaaccattg agctggaaca
     4621 gaacgaaatt cgttcgctct tacctgaagc cagtgagccg attgatcaag ctgcacaaga
     4681 agatgaagcc attccccagg atgaactgga tgacaaaatc gccggtgaag cgggcgtgca
     4741 tgaatatgtt gtttccactg gcgatacgct aagcagcatt ctcaatcagt atggtattga
     4801 tatgggtgat atcacccaac tggctgctgc cgacaaagaa ttgcgtaacc tgaaaatcgg
     4861 tcaacaactc tcctggacat taaccgcgga cggcgaactg cagcgcctca cctgggaagt
     4921 gtctcgtcgt gaaacccgaa cctatgaccg tactgccgct aacggtttta aaatgaccag
     4981 cgaaatgcag caaggagagt gggtcaacaa tctgctaaaa ggtaccgtcg ggggaagctt
     5041 tgttgccagc gccagaaacg ccggtttaac cagcgccgaa gtgagcgcag tgattaaagc
     5101 catgcagtgg caaatggatt tccgcaaact gaaaaaaggc gatgaatttg cggtgttaat
     5161 gtcacgagaa atgcttgatg gtaaacgtga gcaaagccag ctgctgggcg tacgtttgcg
     5221 ttcagaaggt aaagattatt acgcaatccg cgctgaagat ggcaaattct acgatcgtaa
     5281 cggtactggt ctggcgaaag gattcttgcg attcccgacg gcgaaacagt tccgtatctc
     5341 atctaacttt aacccgcgtc gtactaatcc ggtgaccggt cgcgttgcgc cacacagagg
     5401 tgttgatttt gccatgccac aaggtacgcc agtgctttca gtgggtgacg gtgaagtggt
     5461 ggttgccaaa cgcagtggcg cagcaggtta ttatgtggct attcgtcatg gtcgcagcta
     5521 caccacgcgt tatatgcact tgcgcaagat cctggtgaaa ccgggacaga aggtgaaacg
     5581 tggcgaccgt atcgcgcttt ccggtaatac cggacgttca accgggccgc atctgcacta
     5641 tgaagtatgg ataaaccagc aggccgtaaa cccgctgacg gcaaaactgc cgcgtaccga
     5701 agggctgacc ggctccgatc gtcgcgaatt cctggcgcag gccaaagaga ttgtgccgca
     5761 gctacggttt gattaattaa catccattcg cagccggtac gcagtcagta ccggcttttt
     5821 ttatttggtg cggggcaagt tgcgccgcta cactatcacc agattgattt ttgccttatc
     5881 cgaaactgga aaagcatgga aacgaaaaaa aataatagcg aatacattcc tgagtttgat
     5941 aaatcctttc gccacccgcg ctactgggga gcatggctgg gcgtagccgc gatggcgggt
     6001 atcgctttaa cgccgccaaa gttccgtgat cccattctgg cacggctggg acgttttgcc
     6061 ggacgactgg gaaaaagctc acgccgtcgt gcgttaatca atctgtcgct ctgctttcca
     6121 gaacgtagtg aagctgaacg cgaagcgatt gtagatgaga tgtttgccac cgcgccgcaa
     6181 gcgatggcaa tgatggctga gttggcaata cgcgggccgg agaaaattca gccgcgcgtt
     6241 gactggcaag ggctggagat tatcgaagag atgcggcgta ataacgagaa agtgattttt
     6301 ctggtgccgc acggttgggc cgtcgatatt cctgccatgc tgatggcctc gcaagggcag
     6361 aaaatggcag cgatgttcca taatcagggc aacccggttt ttgattatgt ctggaacacg
     6421 gtgcgtcgtc gctttggcgg tcgtctgcat gcgagaaatg atggtattaa accattcatc
     6481 cagtcggtac gtcagggcta ctggggatat tatttacccg atcaggatca tggcccagag
     6541 cacagcgaat ttgttgattt ctttgccacc tataaagcga cgttgcctgc gattggtcgt
     6601 ttgatgaaag tgtgccgtgc gcgcgttgta ccgctgtttc cgatttatga tggcaaaacg
     6661 catcgtttga cgattcaggt gcgcccaccg atggatgatc tgttagaggc ggatgatcat
     6721 acgattgcgc ggcggatgaa tgaagaagtc gagatttttg ttggtccgcg accagaacaa
     6781 tacacctgga tattaaaatt gctgaaaact cgcaaaccgg gcgaaatcca accgtataag
     6841 cgcaaagatc tttatcccat caaataaaaa atgcctctcg cgaggagagg ccttcgactg
     6901 gtgataagtt caagtttgct tcagaagatt cgaaatctgt tgaattatca ttgaactgta
     6961 ggccggatga ggcgttttcg ccgcatccgg caacgtactt actctaccgt taaaatacgc
     7021 gtggtattag tagaacccac ggtactcatc acgtcgccct gggtgacaat caccaggtca
     7081 ccagacatca agtaaccttt atcgcgcagc agattaaccg cttcgctggc agccgctacg
     7141 ccgtcattag cgctatcaaa gtgcaccggc gtaacgccgc gatagagagc agtcaggttc
     7201 agcgtacgtt catggcgtga catggcgaaa attggcagac cagagctgat acgggaggtc
     7261 atcagcgcgg tacgacccga ttcggtcatg gtgatgatcg ccgtaacgcc tttcaggtgg
     7321 ttagccgcgt acattgctga catggcaatg gcttcttcca cattgtcgaa ctgaacgtcc
     7381 agacggtgtt tagaaacgtt gatgctcggg attttttccg cgcccaggca aacgcgtgcc
     7441 atggctgcaa cggtttctga cggatactga ccagcagcag tttctgcaga aagcatcaca
     7501 gcgtcagtac catccagaac ggcgtttgct acgtccatga cttctgcacg cgtcggcatc
     7561 gggttagtaa tcattgactc catcatctgg gtcgccgtga ttactgcgcg gttaagctga
     7621 cgcgcacgac ggatcaacgc tttctgaatg ccgaccagtt ccggatcgcc aatttccaca
     7681 ccgaggtcgc cgcgagcaac cattaccacg tcagaggcga ggatgatgtc atccattgca
     7741 tcctggctgc aaacggcttc cgcacgttca accttggcaa caattttcgc atcacatcct
     7801 gcatcgcgtg ccagccgacg ggcatagttc agatcttcgc cgcagcgtgg gaaggagaca
     7861 gccaggtaat ctacgccaat taacgctgca gtcttaatgt ctgctttgtc tttttcggtc
     7921 agcgcttcag ccgacaaacc gccgccaagt ttgttgatac ctttattgtt ggagagggga
     7981 ccaccgacgg tcacttcggt gaacactttc atgccctgaa cttccagtac ttttaactga
     8041 acgcgaccat cgtccagcag caggatgtca ccaggcacga cgtcagcagg caggcctttg
     8101 tagtcgatac cgactttttc tttgtcgcct tcacctttac ccaggttggc gtcgagcagg
     8161 aatttatccc caatattgag gaaaactttg ccttctttaa aggtggatac acgaattttg
     8221 ggcccctgga ggtcacccag aatagccaca tgacgcccca gttttgcggc aatttcacga
     8281 actttatccg cgcgcatttt gtgatcttca ggcgagccgt gagaaaagtt catacgtaca
     8341 acgttggcac ccgctgcgat aactttttca aggttattat cgcgatctgt tgctgggcct
     8401 aacgtggtaa cgatttttgt tctgcgaagc cttctggaca tgtaatactc cgttgactga
     8461 aacaaccagg tgttgcttga acatgaaatc cgaatgaaat aacgccgcga tggcgataaa
     8521 ttaatctcat tcggatgtcg tataagatta ggacagtgac agtcgttttt agcgatcgtc
     8581 acttaaatta agtagctgct tatcaaaacg cgattccttc agcgcttcct tgacccgctt
     8641 caagttatct ctgaattttg caccgcgtcg caaagtaaat cctgtcgcca gcacatctat
     8701 cacggtcagc tgcgcaagtc gagaaaccat gggcatataa atgtcagtat cttccggtac
     8761 gtcgagggta attgccagcg ttgcttcccg ggcgagcggg gtacccgcag aggtgagggc
     8821 aatcactatg gcgtcgtttt cgcgtgccag ctgcgccagc tcgaccagat tttttgttct
     8881 tcccgtgtga gaaatcagca ccaccacgtc tccgtcgcta caattcatac aactcatgcg
     8941 ttgcagcacg atatcatcgg agtacaccac cggaacatta aaacgaaaga acttattcat
     9001 cgcatcgtgg gcaacggcgg ctgaagagcc taatccgaaa aaggcgattt tttttgcctg
     9061 agtgagcaaa tcgacggcgc ggttgatggc agatttatcc agtgaatgac ggacatgatc
     9121 gagcgttgcc attgcggact caaatatttt ccccgtgtat gattcaacgc tgtcatcttc
     9181 attgacgttg cgattaacat agggagtgcc attcgccaga ctttgtgcca gatgaagttt
     9241 aaaatcagga aaaccgcgag tgtccatgct gcgacagaaa cgattcaccg tcggttcgct
     9301 aacattggct tccagcgcca gagcagcaat actcgaatgg atcgcgttat cgggcgaagc
     9361 cagaatgacc tcggcaactt tgcgctcaga tttgctcaaa tgttccagct gagactggat
     9421 tttttccagc atattcatga tgtaaagaga ctcacgggta atgacgattt ccgctctgaa
     9481 agaaatcgaa atgcagtttt gtcagatatt acgcctgtgt gctgtgttaa tgacaaaagc
     9541 agataa
//
LOCUS       DAZGAP010000185         9095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGAP010000185 DAZGAP010000000
VERSION     DAZGAP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.186"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(153..1313)
                     /locus_tag="ACHLAM_004255"
     CDS             complement(153..1313)
                     /locus_tag="ACHLAM_004255"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7855703.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1319..1990)
                     /locus_tag="ACHLAM_004256"
     CDS             complement(1319..1990)
                     /locus_tag="ACHLAM_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO7855704.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCIAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2138..3619)
                     /gene="tolC"
                     /locus_tag="ACHLAM_004257"
     CDS             complement(2138..3619)
                     /gene="tolC"
                     /locus_tag="ACHLAM_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO7855705.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3824..4453
                     /gene="nudF"
                     /locus_tag="ACHLAM_004258"
     CDS             3824..4453
                     /gene="nudF"
                     /locus_tag="ACHLAM_004258"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO7855706.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4454..4876
                     /locus_tag="ACHLAM_004259"
     CDS             4454..4876
                     /locus_tag="ACHLAM_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO7855707.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4901..5728
                     /gene="cpdA"
                     /locus_tag="ACHLAM_004260"
     CDS             4901..5728
                     /gene="cpdA"
                     /locus_tag="ACHLAM_004260"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO7855708.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            5728..6309
                     /gene="yqiA"
                     /locus_tag="ACHLAM_004261"
     CDS             5728..6309
                     /gene="yqiA"
                     /locus_tag="ACHLAM_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO7855709.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6338..8230
                     /gene="parE"
                     /locus_tag="ACHLAM_004262"
     CDS             6338..8230
                     /gene="parE"
                     /locus_tag="ACHLAM_004262"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO7855710.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8275..>9095)
                     /locus_tag="ACHLAM_004263"
     CDS             complement(8275..>9095)
                     /locus_tag="ACHLAM_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFO7855711.1"
                     /translation="ALSYAQVKNIVTGEYSAVSKIITEKETLYPSDKILLVFDIDNTL
                     LTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISMLYELSPMQLTEPQLPTML
                     KQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRKKEDTLPPFEKGKLKRSW
                     LYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQANIHAMTKMFSKDKWSSTD
                     FTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLATLFPDRNKLCPIK"
BASE COUNT         2140 a         2221 c         2421 g         2313 t
ORIGIN      
        1 attacaatat cgcttgcata tgacgattta acattaaatt gagtattaat gttaaataaa
       61 ggttggatat gtatgtgttt atatattgat gggaattatg aatggtggat tataagagat
      121 tgccccgctt atcggggcaa tattaagctg tattactcaa caaaaatatg cggataaaac
      181 cgagacatat cctgggtgat caatgcacgg tcttcacgaa tgccaattcc ggcaggttga
      241 tcgttcacca gccagctacc aatcagcata tagctgtcgc cgaatttcgg taacgggtgg
      301 aattgctgaa caatcatccc ttcttcgcca tacggacctt ccgctgcttc aatggttttg
      361 ccgttctcaa tgatcgacac gtttgcgcct tcacgggaga agatcggttt aaccacatat
      421 ttttccattt gcggatgatc atcttccgca aaataagcag gcagcaggtt cgggtgattc
      481 gggaacatct cccacagtag cggcagaagc gctttgttgg agataatgct cttccacgcc
      541 ggttccagcc agcgtacgcc tgcatcctcc agcttggtgg agaacatctc gcgcagcata
      601 aattcccacg gatacagctt gaacaggttg gaaattacct gatcctgtaa atccgtgaac
      661 tgaccttttt cgcctaaacc gatatcgtcg atgtagagga attcggtagc gatttcagct
      721 tccgtcgcgc agtcctgcaa atactgaatg gttccgcgat cttccaccgt gtcgcgacag
      781 caggtgagat gcagcaactg gaagccatac tgttcacgca actcgacaaa gcgatcgatt
      841 agcttttctt gcagactgtt aaactggtcg ctgccctccg gcaagttacc ggcgttaagc
      901 tgatcttcca gccagatcca ctgaaagaac gccgcctcgt atagtgacgt tggtgtatcg
      961 gcgttatttt ccagcagttt aggttcgcca gtgccatccc acgccagatc aagacgcgaa
     1021 taaagcgatg gctggtgagt cagccatgac tgacgcacaa aactccaggt gtgttttgga
     1081 atgcggaatt tggtcatcag ctcatcgctg gcgatcactt tttccaccac tttcaggcac
     1141 atctggtgca gttcggcggt gacttcttcc agcttttcaa cctgggcgag ggtcaacttg
     1201 tagtaagcat cttcacacca gtacggctcg ccgtacatgg tgtgaaaatt gaaaccgtat
     1261 tcgtgggctt tctcacgcca gtccgggcgc tcggtaatac tgactctttc catcggtatc
     1321 agccacccat tgaacgagaa gaggtgccgg ttgcgctacg ctgcatagtg ctttgtttgg
     1381 caacagattc accaaaaccg ccacgggtaa cggtagtggt ggtcgccggt tttggtgcca
     1441 tggctgtctt cggtacggtc atggtgcggc ctggctgggc tgcgccatag tttttacccg
     1501 tcgcgtcggt atatttaccg taagccgggc tggctgggtt tttcgaggag aacagcggct
     1561 gctgtgcaaa tcccgcaccg ccgcccatca gacgacccat catgtaaccg gccatcagcg
     1621 gcatccagaa actcccgctg gattgctggg cctgcgcctg gttttccggt gccatgccag
     1681 cctgggctgg tgcctgctgg cactgacctt cgccaaattc agcaatacag tcttcacgag
     1741 tggcgtattt cggcgcagta cgttcggctt ctttcagcgc attgttgtac gcggtggtac
     1801 attcggcgct tttgcctggg tttgcagctg aacagtcgtc agcattttga tagagagaca
     1861 ctgtttcatc actcttttca cagccagcca gcataaaaac agtggcaacc gcgagagcga
     1921 ctggtgtcag atggcgtgcg ctccagtttt tgcggaacga tgcgtggcgt atggattttg
     1981 tccgtttcat ttttgtcttc cgggaccagt ggtaaatacc catcagaata gaggatggct
     2041 ggtcgaaatt gaagcgagaa gggggaagaa tgcggcagat aacccgtatc tttacgttgc
     2101 cttacgttca gacggggccg aagccccgtc gtcgtcatca gttacggaaa gggttatgac
     2161 cgttactggt ggtagtgcgt gcggatgttt gctgaacgac gggtgccggg ctatcaggcg
     2221 cataaccatc agcaatagca ttctgttccg gcgtttgcgg ggcaacgttt tccggattag
     2281 tggaaaccgg tttgctcagc gcattgttca gtgccagaag atcctgctcg ttcaacgtac
     2341 ccagagctga tttaatattc aactggttaa tcaggtagtt ataacgcgca ttcgccagct
     2401 cttgcttggc gttgtacagc gtggtggtcg catccaacac atcaacaatg gtacgcgtac
     2461 cgaccgagta acccgcttcc atcgcgtcta atgagctttg agcggaaact acggcttgtt
     2521 tgtaggcgtt aatgctactg atagaagcat taatgttgtt gaaggaggaa cgtacggttt
     2581 gcacgacgct acgatgcgcg ctttccagtt gctcgctggc accaacaaag ttgtactggg
     2641 cctgtttcac ctgcgagtta accattccgc cctgataaat cggcagcgag aagctcaggc
     2701 caactttgtt ctggcccata ttgctgtcgt catactgggt accagcggca ccacgggttt
     2761 tcgaaccgct ataagaggtg tcagaaatcc cggtagaagc cgttaaatcc agagtcggta
     2821 agtgaccatc ctgcgcctgg cgaatttgct cgcgcgccag gtcctggctc aagcgcgcct
     2881 gtaacagcga caggttgcgt ttttcggctt ctttcagcag cgtgttaacc ggctgtggtt
     2941 tgtcggtttt aaagttttcg acattcagcg ccgccagttc tggatagtag ttaccggtga
     3001 tctggcgcag ctgctctacc gcgttatcaa ggttattacg tgcggtcact tcgttcgcca
     3061 gcacggtatc gtactgtgcg cgggcgttct gcacgtcagt gatcgctacc aggcccacgt
     3121 taaaacgttg ggtggtttga tctaattgac ggtagatcgc ttctttttgc gcctgtgtat
     3181 aggaaagaac gtcaatagca ttcaacacgt tgaaataagc ggtcgcggtg ttgaggatca
     3241 aggtttgttg atcggtctga tacgtgacgt cctgaatccc agctgctttt tcctgcagcg
     3301 ttaacgcacg ccatttcgac atatcaaaaa tggattgagt taactgcagg gacgcactgg
     3361 tcgcgttaga gttgatgccg ttcgcgtcgc ggtagccgtt gctataggtg taatctgcac
     3421 ctaaacctag ctgtggcagt aatggactgc gcgcttcatt aattttttca aaggcagcat
     3481 cacgatcggc ggcagactta cgcaattccg ggttactaag gcgtgcttgc tgataaactt
     3541 gcatcaggtt ctcggcctgg ctcaacgaac tgaaaccaga aaggctcagg ccgataagaa
     3601 tggggagcaa tttcttcatt tgcattcctt gtggtgaagc agtatttagc gcgatcaaac
     3661 tgtaaaatta ttgcggattc tagcagaagc cgctaccgca aaaggttggc gttacgtgcc
     3721 atagggcgtt aatgtgcctc aatttaacac atgactgctc aaacggcagt caaacgtcgc
     3781 tgaaattcac atttaattca ctattagtgc caggacatta acaatgctta agccagacaa
     3841 cctgcccgtt acatttggca aaaacgatgt agaaattatt gcacgagaaa cactttatcg
     3901 cggctttttt tcattagatc tttatagatt tcgtcatcgt ctattcaacg ggcaaatgag
     3961 tcatgaggta cggcgggaaa tttttgagcg cggtcacgcc gcagtcttgc taccctttga
     4021 cccagtgcgt gatgaagttg tgctgattga gcagattcga attgccgcgt acgacactag
     4081 cgaaaccccc tggctactgg agatggttgc cgggatgatt gaagagggtg aaagtgtgga
     4141 agatgttgcc cgtcgcgaag cgattgaaga ggcgggactg atagtcaaac ggaccaaacc
     4201 ggtgttaagt ttcctggcaa gcccgggggg caccagtgag cgttcgtcaa ttatggtggg
     4261 cgaagtggac gccacgaccg caagcggtat tcacggtctg gctgatgaaa acgaagatat
     4321 tcgcgttcat gtggtaagcc gggaacaggc ataccagtgg gtagaagagg ggaaaatcga
     4381 caacgcagcg tcggtcatcg ctttgcaatg gctgcagctg catcatcaag cgttaaaaaa
     4441 tgagtgggca taaatgaagc gttacacacc tgactttcct gaaatgatgc gcctgtgcga
     4501 gatgaacttt tcacaattgc gccgtttgtt accgcgcaat gacgcacccg gcgaaactgt
     4561 aagctatcag gtggcaaacg cacaatatcg gctgacgatt gtggaatcga cccgatacac
     4621 taccctggtg acaatagaac agactgcgcc cgcgatcagt tactggagcc ttccgtcaat
     4681 gacggtgcgt ctgtatcatg acgcgatggt ggctgaagtg tgttcaagcc agcagatttt
     4741 tcgcttcaaa gcgcggtatg attatcctaa taaaaagttg catcaacgcg acgaaaagca
     4801 tcaaattaac cagtttttag cggactggtt gcgatactgt ttagcacatg gagcgatggc
     4861 gattccggtt tactagcgtc gtgaaaccta aggacaccat ttggaaagcc tgttaaccct
     4921 tcctctggct ggtgaggcca gagtcaggat tttacaaatt accgacactc acctgtttgc
     4981 acaaaagcac gaagccctgt taggggtaaa cacctgggaa agctaccagg cggtgctgga
     5041 ggcgattcgt ccacaccagc acgaattcga cctgattgtc gcgacaggtg atttagcgca
     5101 ggatcaatcc tctgcggcct atcagcattt cgctgaaggc atcgcaagtt ttcgtgcgcc
     5161 ctgcgtctgg ctgccgggca accacgattt ccagcccgcg atgtacagcg cgttacagga
     5221 tgcgggtatc tccccggcga agcgggtgtt tattggtgag caatggcaaa tcctgttgct
     5281 ggatagccag gtgtttggcg tgccgcacgg tgagctgagc gagtttcagc ttgagtggct
     5341 ggaacgtaaa ctggccgatg cgccagaacg ccatacgttg ctgctgctgc atcatcatcc
     5401 gctacctgcg ggttgtagtt ggcttgatca acacagcctg cgtaacgcgg gcgaactgga
     5461 taccgtgctg gcgaagtttc cgcacgtcaa atacttgctg tgcggtcata ttcatcagga
     5521 gctggatctc gactggaacg gtcgccgcct gctggcaacg ccgtcgacct gtgtgcagtt
     5581 taagccgcac tgttccaact ttacgctgga caccatcgcg cccggctggc gtactctcga
     5641 gttacatgct gatggcacgc tgaccaccga ggtgcatcgc ctggcggaca cacgtttcca
     5701 acctgatacc gcttcagaag gctactgatg tctacgcttc tttatttaca cggtttcaac
     5761 agctcgccgc gctctgcaaa agcgagcttg ttaaaaaact ggcttgcgga acatcaccct
     5821 gacgttgaga tgatcatccc gcagttgccg ccgtatcctt ccgatgcggc agaactgctg
     5881 gaatccattg tcctggaaca tggcggcgat tcgctgggta ttgtcggttc gtcactgggg
     5941 ggatattacg ccacctggtt gtcgcaatgt tttatgctgc ccgcagtggt ggtaaacccg
     6001 gcggtacgcc cgtttgaact gctgacggac tatctcggtc agaacgagaa cccctacacc
     6061 gggcagcaat atgtgctaga gtcacgccat atttacgatc tcaaagtcat gcagattgac
     6121 ccgctggaag cgccggattt gatctggctg ctgcaacaga cgggagatga agtgctggat
     6181 taccgccagg cggtggcgta ctacgcttcc tgccgccaga ctgtcataga aggcggcaat
     6241 cacgcattca cgggcttcga agattatttc aacccgatcg tcgattttct tggtctgcac
     6301 catctctgac gatcaactcg aattgctaac ttaaaccatg acgcaaactt ataacgctga
     6361 tgccattgag gtactcaccg ggcttgagcc agttcgccgc cgtccgggga tgtataccga
     6421 taccactcgc cctaaccatt tggggcaaga agttattgat aacagtgtcg atgaagcact
     6481 ggcgggtcac gcaaaacgcg tggacgtaat cttacatgct gaccagtcgt tagaagtgat
     6541 tgacgatggg cgcgggatgc cggtggatat tcacccggaa gagggtgtac cggcggttga
     6601 actgattctt tgccgtctgc atgcaggcgg taaattctct aacaaaaatt accagttctc
     6661 tggcggcctg catggcgtgg ggatttcggt ggttaacgcc ctgtcgaagc gcgtagaagt
     6721 taacgtacgc cgcgatggtc aggtttataa catcgccttt gaaaatggcg aaaaggtgca
     6781 ggatttacag gttgtcggca cttgcggtaa gcgcaatacc ggtaccagcg tgcacttctg
     6841 gccggatgaa accttctttg acagcccgcg tttttctgtt tcacgcctga cgcatgtgct
     6901 gaaggccaaa gcagtactgt gccctggtgt tgagatcacc tttaaagatg agatcaacaa
     6961 caccgaacag cgctggtgct atcaggacgg tctgaatgat tacctggcgg aagcggtaaa
     7021 cggtttaccg acgctgccag aaaaaccgtt tatcggtaat ttcgctggcg atactgaagc
     7081 ggtggactgg gcgctactgt ggctaccgga aggcggtgaa ctgctgaccg aaagctacgt
     7141 caacctgatc ccaacgatgc agggcggtac ccatgttaat ggcctgcgtc agggcctgct
     7201 ggacgcgatg cgtgaattct gtgaataccg caacattctg ccgcgcggtg taaagctgtc
     7261 ggcggaagat atctgggatc gctgcgccta tgtgctgtca gtaaaaatgc aggatccgca
     7321 gtttgccggg cagaccaaag aacgtctctc ttcgcgtcag tgtgcggcat ttgtttctgg
     7381 cgtggtgaaa gatgccttca ccttgtggct gaaccagaac gttcaggcag ctgaactgct
     7441 ggcggagatg gcgatttcca gcgcccagcg tcgtatgcgt gcggctaaaa aagtggtgcg
     7501 taaaaagcta accagcggcc cggcgctgcc tggtaaattg gctgactgta ccgcgcagga
     7561 tcttaaccgt accgaactat tccttgtgga aggtgactcc gcaggcggat ctgccaagca
     7621 ggcgcgcgat cgcgaatatc aggcgatcat gccgctgaaa ggtaagatcc ttaacacctg
     7681 ggaagtctct tccgacgaag tgctggcttc gcaggaagtg cacgatattt cggtagcgat
     7741 cggtatcgat cctgacagtg acgatctgag ccaacttcgt tacggcaaga tctgtatcct
     7801 ggcggatgct gactccgatg gtctgcacat tgccacgctg ctctgcgctt tgtttgtaaa
     7861 acacttccgc gcattggtga aacacggtca cgtttacgtt gcactgccac cgctctaccg
     7921 tattgacctc gggaaagagg tttattacgc gctgacggaa gaagagaaag agggcgtact
     7981 tgagcaatta aaacgcaaga aaggcaagcc aaacgtccag cgttttaaag gtctcgggga
     8041 aatgaacccg atgcaattgc gcgaaaccac gcttgatccg aacactcgcc gtctggtgca
     8101 gttgactatc gatgatgaag acgatcagcg tactgacgcg atgatggata tgctgctggc
     8161 gaagaaacgc tcggaagatc gccgcaactg gttgcaagaa aaaggcgaca tggcggagat
     8221 tgaggtctga taatcttaac ccggcaaaca tgtgttttgc cgggttgtcg ttgattattt
     8281 tattgggcac agtttattac gatctggaaa tagtgttgcc agcgaaccag ccagagtttt
     8341 ccagtccgta gccatttttt ctgcttgtgc agttgttatt actgcacctt gttgtttaat
     8401 aagctcatcc tccactctgg tatagtgaat aatggtaaaa tccgtagagg accatttatc
     8461 cttagagaac atcttcgtca tcgcatgaat gtttgcttgc ccgtcatcga taaaaacaat
     8521 cgcgtcatat ttgttcccca ttttatccag catataatcg agaattacgc ctttatcttg
     8581 cccggaagaa aaaaataccc cctggctgta cagccatgac cgtttgagtt tccctttttc
     8641 aaagggtggc aaagtatcct ctttcttcct gagtgcggaa gacgaaaaat caatgccgtt
     8701 tcgctttaat tcccgcaatg tcggaaagag agtatccggt gcacgggatg tcagagcaaa
     8761 agcggtatgc ttttgttgcc actgttttaa cattgtcggt aactgtggct cggtaagctg
     8821 catagggcta agttcgtaaa gcatactgat tgtattttca tacaggcaag gaactttttg
     8881 cgagtcatcc ggctttaaag gcaatttatc ggtttgccat tgataccaga tatcaccgcc
     8941 aatgcgagtg ccactcgtta ataacgtgtt atcaatgtca aaaaccaata atatcttatc
     9001 ggatggatag agagtttcct tttctgtgat aattttactg accgcactat attcgccagt
     9061 aactatattt tttacctgtg cgtaagataa agcag
//
LOCUS       DAZGAP010000186         9065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGAP010000186 DAZGAP010000000
VERSION     DAZGAP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.187"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..76
                     /gene="rcsA"
                     /locus_tag="ACHLAM_004264"
     CDS             <1..76
                     /gene="rcsA"
                     /locus_tag="ACHLAM_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="with RcsB is a positive regulator of capsular
                     polysaccharide synthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO7855712.1"
                     /translation="NKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(120..308)
                     /gene="dsrB"
                     /locus_tag="ACHLAM_004265"
     CDS             complement(120..308)
                     /gene="dsrB"
                     /locus_tag="ACHLAM_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO7855713.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            471..698
                     /gene="yodD"
                     /locus_tag="ACHLAM_004266"
     CDS             471..698
                     /gene="yodD"
                     /locus_tag="ACHLAM_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO7855714.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            997..1812
                     /gene="yedP"
                     /locus_tag="ACHLAM_004267"
     CDS             997..1812
                     /gene="yedP"
                     /locus_tag="ACHLAM_004267"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO7855715.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAKQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(1809..3503)
                     /gene="dgcQ"
                     /locus_tag="ACHLAM_004268"
     CDS             complement(1809..3503)
                     /gene="dgcQ"
                     /locus_tag="ACHLAM_004268"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO7855716.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(3674..3856)
                     /gene="yodC"
                     /locus_tag="ACHLAM_004269"
     CDS             complement(3674..3856)
                     /gene="yodC"
                     /locus_tag="ACHLAM_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO7855717.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(3935..4852)
                     /gene="yedI"
                     /locus_tag="ACHLAM_004270"
     CDS             complement(3935..4852)
                     /gene="yedI"
                     /locus_tag="ACHLAM_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO7855718.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5025..5945
                     /gene="yedA"
                     /locus_tag="ACHLAM_004271"
     CDS             5025..5945
                     /gene="yedA"
                     /locus_tag="ACHLAM_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO7855719.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(5934..6404)
                     /gene="vsr"
                     /locus_tag="ACHLAM_004272"
     CDS             complement(5934..6404)
                     /gene="vsr"
                     /locus_tag="ACHLAM_004272"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO7855720.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(6385..7803)
                     /gene="dcm"
                     /locus_tag="ACHLAM_004273"
     CDS             complement(6385..7803)
                     /gene="dcm"
                     /locus_tag="ACHLAM_004273"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO7855721.1"
                     /translation="MQENISVTDSYSTGNVVQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEVKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(7870..8565)
                     /gene="yedJ"
                     /locus_tag="ACHLAM_004274"
     CDS             complement(7870..8565)
                     /gene="yedJ"
                     /locus_tag="ACHLAM_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO7855722.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEEARRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(8605..8886)
                     /gene="drpB"
                     /locus_tag="ACHLAM_004275"
     CDS             complement(8605..8886)
                     /gene="drpB"
                     /locus_tag="ACHLAM_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO7855723.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         2180 a         2464 c         2218 g         2203 t
ORIGIN      
        1 taataaacag gttatctacc atgtcgtccg actgacggat aatgtgacta atggtatttt
       61 tgtcaacatg cgctaacaca ttctgactgg tggtttccca ccagtcaggc tgaattagat
      121 tactctgctt tctccacaaa gataccgtcc tgatgccctg cttcattaaa gaaccagatg
      181 ccgagcgggt agtcttccag cgaaaccagg tacattgtgc cttcactaaa ctcctcaact
      241 gccagtacca cgccaggacg acgcggaccg ccatccgttt tgactgttac ccgatcattc
      301 accttcatcg ttttcctcct gtggctttgt gccagtgtag aacaatttcg tttttctggc
      361 agcgccaggc gcgcgcgagt gctgattttc tcgacggtct atacttaaga gatgccaggc
      421 ggacttaacg actggcggca acaacagagt aacggttgcg aggaaagatg atgaaaaccg
      481 caaaagagta cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat gccctgctgg
      541 cggcgatcaa tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat tctgaacacg
      601 ttagtgtcga tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg
      661 atattcatga cttcagcgtc tctgaagtga atcgttgaat gcacaataaa aaaaatcccg
      721 accctgaggg ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca
      781 ccaggaaatc tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat
      841 tcgtgatatg aatgattaac gctacttatg aatagaaatg tgacgcgcgt caccccttgg
      901 tggctctggc gtcaacggga tcgctcccaa aaaaatcatc tttgataatt tgtgctgttt
      961 tgagaatcat cctggtaagg ggagtactgc gcaaccatgc tttcaattca acaaccacta
     1021 ctggttttta gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg
     1081 gcagccccct ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt
     1141 aaaacttcag cggaaatgct gtacttgcaa aaaatgttgg ggctacaagg cttaccgctg
     1201 attgcagaga atggcgcagt gatccagctt gctaagcaat ggcaggagat agacggtttt
     1261 ccacgcatca tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc
     1321 gagaaagaac attttaaatt cacgactttt gatgatgtcg acgatgcaac catcgccgaa
     1381 tggacgggat taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg
     1441 ctaatctggc gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg
     1501 ggcttgcagt ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa
     1561 gatcaggctg ccaactggat tatcgcgacc tatcaacaat tgtcaggcaa acgcccaacc
     1621 acacttggcc tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg
     1681 gtgattgtga aagggctaaa ccgtgaaggg gtccatctgc atgatgagga tccggcccgc
     1741 gtctggcgaa cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc
     1801 tccgcccgtt aagcgttatc gctcgcgaat acccgattgc gcccggcctg ttttgcgagg
     1861 taaagccgac ggtcggccaa tgactggagt tgttcaaaat cataatcacc ggtttcctcg
     1921 ctgctgctta cccccagcga ggcactgatg cgtatcgtcg tactcttggc gatcagcatc
     1981 tctttttcat ttaacttcag gcgaatacgt tctgcgacct ccgcagcctg cgtcagattc
     2041 gcgcctggca gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca
     2101 cgtaaggaac tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cacctgatga
     2161 ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga cctgaatgac agaaaatgga
     2221 tgttggtgcg tctgacacaa tttagcgagc ggacgggctt tttcgaacag tgcgccacgg
     2281 ttatataaac gcgttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca
     2341 tacatgttgc tgaccatccg gcgaatcaca taccaggaga gtagtaacat ggtggtaaag
     2401 agcgcccaca gcagggttaa tgcaatgctg atactgccga aatcaccacg cacgccttcg
     2461 cttagcgtat ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca
     2521 taacgactgt tcatacgaat gccaccccgc gtgtcatgtt ccattgcctg cgccaacaag
     2581 gccagttcac gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa
     2641 aatctcagct tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt
     2701 cttaaaaatt gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag
     2761 ttttcactgt ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc
     2821 ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata
     2881 aaccagggtt gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc
     2941 ggctgcgtcg aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac
     3001 gaggagttgt gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg
     3061 tcgaggcttt cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgacacg
     3121 ccattgactg gcagggtgcg tcgtcggttg agttcgattt gccaggcgtg ctcgtcgcga
     3181 tgctgttcga actcggttac ggcattacgc agagaggtga aatccagtgg cgctacgaga
     3241 gcttcgcgca tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta
     3301 tcgagcgcgt tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc
     3361 agcaccacca cttcacgcca ggtgagcaag gtggaaaaaa gcaataccac gataaagcag
     3421 agattaacga catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt
     3481 tccatttttg tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc
     3541 ataacccggc gataacgtac cgcaaaacag cgataatttt ataatggtgg catgataaga
     3601 cgcgactatc attcattgca ggcattggca caccaggccg gatgtgatcc ggcctggtgt
     3661 taatgatacc ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagatcgg
     3721 tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac
     3781 cgctggagta tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac
     3841 taaccataaa gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa
     3901 gatggcgaat tttgcataat agccagtgcg agtattaatg tgcctgaccg cgcattttcg
     3961 ctactgcttt cactcccagc accacgatgc cgccgatgat aaaaccaaga atcagattta
     4021 aaacagtcgg taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg
     4081 cgtgatgcag cggcgcaata ccatgtacca caatcccgcc gccgacgagg aacatcgcca
     4141 gcgtgccgac aatcgataat gctttcatca gccagggcgc gataatcaat aaccctttac
     4201 ctaatgcctg catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct
     4261 taacgataac ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa
     4321 gcaccagcac ctgattaagc aacggcgcct ctgccacaat ccccagcgtg atggcaacga
     4381 tttccgcaga caaaataaaa tcggtacgaa tcgccccttt aattttgtcc ttttcaaact
     4441 tcagcggatc ctgcgccgcc agcttctcca gacgctgctg actctgcgcc ggatcttctt
     4501 tatgtttacg cgcctccagc atatgcagca ctttctctac cccttcaaag cagagaaacg
     4561 cgccgccaat catcaacagt ggcgtaatcg cccacgggat aaacgcactg atgatcagcg
     4621 ccagcggcac cagaatcact ttattgatca gcgatccttt cgccacgccc cagaccacgg
     4681 gaagttcccg gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc
     4741 ctaatacacc ggcggttttc ttcgccgcca gtttgcccat cacggaaata tcgtccagca
     4801 gtgtggcgat atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc
     4861 atttttattg ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca
     4921 caggtcaaca tttttttaac aatcaagtgc aattatatct ctcgccagca gaatgatttt
     4981 cacgtttact atgacccacc tttttatttc agctgtgagg gattatgcgt ttccgccagt
     5041 tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg
     5101 tcattcggat tggcgtggaa agctggccgc cgttaatgat ggcgggcgtg cgattcctgg
     5161 cagccggtat tttattgctg gcatttttgc tactgcgcgg acacaaactt cccccgctac
     5221 gtccgctgct caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg
     5281 tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc cgccgtagtg gttgcaaccg
     5341 tgcccctctt taccctgtgc ttcagccgcc tgtttggcat taaaacgcgc aaactggaat
     5401 gggtgggtat cgccattggg cttgccggaa tcatcatgct caatagcggt ggaaatttaa
     5461 gcggcaatcc gtggggcgcg attctgattt taatcggctc gattagctgg gcgtttggct
     5521 cggtttatgg ctcgcgcatt accttacctg tagggatgat ggcgggtgcg attgagatgc
     5581 ttgcggcagg cgtggtgtta atgattgcgt cgatgattgc gggtgaaaaa ctgacggcgc
     5641 tcccttccct ttcaggcttc cttgcggtcg gctatctggc gctgtttggt tcgattatcg
     5701 ccattaacgc ttatatgtat ttaatccgca acgtcagtcc ggctctcgcc accagctacg
     5761 cctacgttaa cccggtggtc gcggtcttac tgggtacggg actgggtggc gaaacgctgt
     5821 cgaagattga atggctggcg ctcggcgtaa ttgttttcgc ggtggtactg gtcacgttgg
     5881 gaaaatatct cttcccggcc aagcccgttg tcgcgccagt tattcaggat gcatcaagcg
     5941 agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac cttcgccgca gatccactct
     6001 tccagacgct cggtaagcgc cgcatccgta agcttctcgc gcccacgtaa cgcacactcc
     6061 cagacaatca gtacgcgcca gccgagttcc tgcaagcgac tgatatcgcg gcgatcgcgt
     6121 tcaacatttt tacctatctt ctccagccag aactcggttc gagtcgcagg cactttaaac
     6181 agatagcagt gatgatgatg ccagaagcag ccgtgggtaa atatcacgca gcgatattca
     6241 tcaacgacaa aatccgggcg tccgggcaga ctggcgtcct gaacgcgaaa cgcaaggccc
     6301 tgcccggtta acaaactggc gaggcgcttc tcaatcgccg tatcacgcgt ggcaatcgcg
     6361 cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga ccatgttgtg cctcttgctg
     6421 acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt tttgccaccg cggcaaagac
     6481 cggcacgacc accgagttac cgaactggcg ataggcctga gtgtccgaaa ccggaatgcg
     6541 gaatttcact tctcctggcg cttcaaaacc cattaagcgc gcacattccc gaggcgttaa
     6601 ccgacgtggg cgatgttgct gattcagcgg atcgtcaaag tctttttcac ccgtggccat
     6661 atcccagccg cgatcgatta aaatttccgc gccatctttg taataacgag cagacagcgt
     6721 acgcgtgacg ctttgcggat tgttcggata aaccattcca taaccgaagc cattaccgcg
     6781 cgcctgatgt tttttcgcat aacgatagag gtacttccac agcaccggcg tcaggatata
     6841 tttcgcctcg accatcgggt ccagcagctg ggccagcgtc actcgctgcg cagggaaaca
     6901 ttcgctgata tcacgcaggg taaaatcggc tttcagattc agatcgcgac gaaaacccac
     6961 cagcacgatg cgttcacggt gctgcggcag gaaatgtttg ccatcgatga ttttcggatc
     7021 gtccggcccg ttatcttctg catcagccac gtcatagccc agttcgtcca gcgtctgcat
     7081 gatgatgcgg aacgttttac cctggtcgtg acttttcagg tttttgacgt tttcgagcac
     7141 aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca tcaaagaaca gcgtgccctg
     7201 agtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc tttttcgata cgccagccag
     7261 cgaaaatggc tgacaaggaa aacccgccag taatacatcg tgttcaggaa tgtgttgacg
     7321 aatatgttcc gccgccgcct catcactcac gccttcttta tggctgaggg tgatgtcgcg
     7381 gatatcttca ttaaaatgat gcgtcgccgg gtcgcaataa tgattggctt tataagtgcg
     7441 taccgcatgt ttgttccatt cgctggtaaa cacgcactgt ccgccaatcg attcaaaacc
     7501 gcgacggatg ccgccaattc cggcaaatag atcgataaag cgaaacgcat aatgcggatg
     7561 atgtgccggt ggtttgggta acaacgtttg cagatgggcg aactctttct cacttaaacg
     7621 gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt gccgcgctcc agtgattctc
     7681 acccacacca ttaagctgcg ccaccagcgt tttaacatca taaatttgca gcagtttctc
     7741 cagcattgcc tgaacgacat tcccggtgct gtatgaatcg gttactgata tattttcctg
     7801 catagattta accggccatc tgagatggcc gacaggttaa cataattaca gccatttagg
     7861 gccacgcgct cagcccgcgg gtgaaaacgc atctatcacc ttgtgatcga caccttcatt
     7921 ctcccccgcc agttcggcac tgagcttcgc cataaactcg actaaaaatt gcgcgttgtg
     7981 ctgcgccagc tgcttgcctc ttgcggtttg catggtttgc ggtagcttca gcaacttagt
     8041 ctggaaatga tccagcgcgt agcgtttatc atccagcggg cgatgctgtg cgaacggatc
     8101 ttcaccatca aacagcgcca cgcccaatgc ccctgaaaca gcaaacacgc gcgccaggcc
     8161 aatcgccccc agggcttcca gccgatccgc atcctggacg attttagctt ccgttgttaa
     8221 gggggcgatt tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg cctcgatttt
     8281 ctccgccgga aattgcacga actcttcacg gagcaggcga cgcgcctctt ctgccgccag
     8341 gattgaagaa tgctgccgtt gcggatgatt tttcgccagg ctcacaatat cgtgaaaata
     8401 acaggcggtt aaaatcacca gcatatcgac atcgtcatct gccgccagct tctgtgccgt
     8461 agcccagacg cggcgaaaat gacatacgtc atgggccgcg tcctggtgct gatgatggtt
     8521 tttcaaccag ttttcgaact gtgcctgcca gtgttgtaag tccatacgcg ctccgttgcc
     8581 aaagagtcgc aaccttagca gtttttattc atagcgtctg ctacgtgcgg gacgaggtcg
     8641 ggtgtaccag gcaatgctgc ctaacaatgc cccgaccaga gccataaata aaaatccgac
     8701 cagtgcccct aaccactttc ccgcggtaga gcctaaatcg ctttcaaatc ccgacaccgg
     8761 ggcatcaggg atccggctca tcacccagat gtaacgcgcc attgcccaga cgaaatagcc
     8821 acaccatgta tagaaagccc acaaagccaa tttgccgcca gggctgcgag agagtctctc
     8881 ttccatcttt gtcgaaccgt attccatttt gagataaccc ggaaaattca gacttagtac
     8941 tattatgtga taaaagtcac atttttccac attggatgag ccagtcaaga ctcaatcatt
     9001 ttttgaacaa atagataatt tttgcgcaac ggtagtgaat acaggaaaaa tcgttttgag
     9061 cgatt
//
LOCUS       DAZGAP010000187         8914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGAP010000187 DAZGAP010000000
VERSION     DAZGAP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.188"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            265..522
                     /locus_tag="ACHLAM_004276"
     CDS             265..522
                     /locus_tag="ACHLAM_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7855724.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            525..1733
                     /locus_tag="ACHLAM_004277"
     CDS             525..1733
                     /locus_tag="ACHLAM_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855725.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGLIFGLRRFVYKAGQVGAESRNVEREALIDSEILRGQRC
                     AWILGTYIQSPAGNKADDLLEAMEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKAL
                     KGAVNKLTTRVEGIVHPLPPALPCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMT
                     GKGLGAFDAWLDKRWDTPGILVVITLSLPASPREEDADAVSMVVLSNRNTHAWPDALC
                     LHRPERGTETTLTKTLTRAFLWAKTRPNELKGSWISGPTLTSGSSWNNACEENGVTFS
                     LSEDNFSIDPVLGYTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKE
                     EVRKESPGNG"
     gene            1726..5097
                     /locus_tag="ACHLAM_004278"
     CDS             1726..5097
                     /locus_tag="ACHLAM_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO7855726.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVASATGFGPY
                     DGLRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPGEE
                     DDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEGDGY
                     VLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALLTDGERDALLRYRQKAD
                     HQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVNGADEAFSTLALHLQKAGM
                     AQILNEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMFSPELTVAGAV
                     PNTCPDTPAWKAIIDDCDAVSGRKLGFNWLKMLRLILLSLIVLWGAGTLLSLLVNRTQ
                     IYQAQETAGQAADTTKPLAERLRSQLVLQQAIARLQHREATGAPWYTRFGLNQDSDTL
                     AALWPLYAKNNAQLMRDATADSLRQQLNAFVQLPPASDARTQGTQHAYDILKSYLMLA
                     RPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFYAQNLPAHPEWKIKPDDEL
                     ISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLADMTGDTDASTVFSTDEVVP
                     GMFTRQAWEEQVQDAINEVVRTRRDEIDWVLTDKTHQTGSDISPEALKSRLTERYFTD
                     FGNAWLNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEAL
                     ADSVVDSAKKLLDKNKNGRQFIEQTQGPEGPLDGVFGPLTGLMEGKEGAGGNGNLSFR
                     SWLARVTQMRLKLQQVTSAPDPQAMSQMLARAVFQGKAIDLTDTCDYGSLVAASLGQE
                     WSGFGQSLFVQPLDLAWRQVLVPAAGSLNARWQTTIVDQWNKAFAGRYPFKATGSDAS
                     LPLLAQFLRSDSGRITTFLKTHLGGLVHQEGNHWVAEPSASQGMVVSPDFLRAINQLA
                     ELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFNQMESWQSFTWPGNTYY
                     PGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASRTQLVWITADGNPLKFV
                     MRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
     gene            5219..5386
                     /locus_tag="ACHLAM_004279"
     CDS             5219..5386
                     /locus_tag="ACHLAM_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855727.1"
                     /translation="MSEKTTDKLTEGQTHSAGERMLLKWRPPQIFNSKDDASPEAVVR
                     FMKETDKQEKE"
     gene            5383..6996
                     /gene="tssA"
                     /locus_tag="ACHLAM_004280"
     CDS             5383..6996
                     /gene="tssA"
                     /locus_tag="ACHLAM_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7855728.1"
                     /translation="MSSPEALLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRD
                     GERGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRETGRE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDVMY
                     GDEAAIPAVVNGQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLKLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            7030..8799
                     /gene="tssF"
                     /locus_tag="ACHLAM_004281"
     CDS             7030..8799
                     /gene="tssF"
                     /locus_tag="ACHLAM_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7855729.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPVGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRF
                     ECPEKVDWSKAGIDKVAIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGW
                     CRPMGFDDNDCLWKKADTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEI
                     DIVLSEAWSSCLPFETENFRLHCAPVINLFTLEADPLLLNPLDNEYLLRPLRLQDGHT
                     EIYSVDNIHGSVKNGRHPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLV
                     LGGRSFELEKLSDKPESLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAP
                     TMPLYPPANDRFHWRVMSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVA
                     VKHTLIRRFEKGFMLRGVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQL
                     TLVLQPTGKRLRWKENHSRYVPG"
     gene            8763..>8914
                     /locus_tag="ACHLAM_004282"
     CDS             8763..>8914
                     /locus_tag="ACHLAM_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7855730.1"
                     /translation="MERESQPVRTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPG"
BASE COUNT         1980 a         2338 c         2590 g         2006 t
ORIGIN      
        1 gtagatagtt ctaaatgtga attttattgc gcactatctg tagtatcgca ttcatatttg
       61 tatgaaattg gcaaaaaata atagtattta ttgataactc aatggtaaat acatgcgatg
      121 gggaacacta tctatcaata atcagtgacc cggaaacatt cataataaaa atccagacaa
      181 tcactatcga tggcagagcc cagcataaac atgggaatta aagagtgatg aattagttaa
      241 gagataagaa agaggaattt aataatgctt catattattc gtaaaggcga taaaactact
      301 cacggaggtt ccgttctgac cgcttctgaa acgatgaaat tcggtgggat aggcgtcgcc
      361 cgtaaaggcg ataaagtgtc atgtccgggt catggtccta ccatcatcat agaaggcaat
      421 cctgattatc ttgaccatgg tatcccggtg gcatttcacg gtcataaatg cgcctgtggt
      481 tgcacattga tcagttcatt tgccgcagca aaggttgcct gattatgcct gtctggctgg
      541 atgccatccc tgaaaaggcc ccaaagatag cgcgccccaa tactcgccgc tggctgttgt
      601 tcctggcatt tatcatgctg ggggggatgg cgctcacatt atggtgctgg acgtcagaac
      661 gaacaggatt tgtcttctgg ttcaccgctc tggggcttcc attttgcacg tgggggctta
      721 ttttcggttt acgtcggttt gtctataaag ccgggcaggt gggggctgag tcccgaaatg
      781 tcgagcggga agcgcttatt gacagtgaaa ttctgcgtgg tcagcgctgt gcctggatcc
      841 tcggtactta tattcagagc ccggccggaa acaaagccga tgacctgctt gaggcaatgg
      901 aagtcgcggc tcctgctatt gatttctcgc acccaagagg atgtgataaa ccggtcaggt
      961 acgcggcatt accagaatac cagacagatt taacgaaggc tctgaaaggg gcggtgaata
     1021 aactgaccac gcgtgtagag gggatagttc atccgcttcc gcccgcgcta ccatgctggc
     1081 tggtgctgga ttgtgataac gatctgtatc ccctgattga agagcagctt aaagccgacc
     1141 tctcactcaa gacgggcagg atttttcgcc tgatgaccgg aaaagggctg ggggcgtttg
     1201 atgcctggct ggacaagcga tgggacaccc cgggcattct ggttgtcatc acgctgtcgt
     1261 tacctgcctc tccccgggaa gaagatgctg atgctgtctc aatggtggta ttaagcaatc
     1321 gcaacaccca tgcctggccg gatgcgcttt gtcttcatcg acccgaaagg ggaacagaaa
     1381 caacgctcac aaagacactt acccgggcgt ttctctgggc gaagacccga cccaatgagc
     1441 ttaaaggaag ctggatctcc ggtcccacat tgacgtcggg cagcagctgg aataatgcct
     1501 gcgaagagaa cggagtgaca ttcagccttt cagaagacaa tttcagtatc gatcccgtgc
     1561 tgggctatac cggccatgct gctccatggc ttgccatcac gcttgctgat gctgcctttg
     1621 aacaacgagg tgcgcaggtt atcgccgccc agcccacagc tgacaaagac gacgtctggg
     1681 ttgccgtcat cactaaagaa gaagttcgca aggaatcacc cgggaatggc taaggtaaaa
     1741 gttaaaacac tcatcggctc tgttgtgact gtcgcctttt ttctgacggt catcgtggca
     1801 tttctttttt atgcctttcc ggaaaacgtc gcttcggcca ccggcttcgg tccgtatgac
     1861 gggctgcgca tactcacatt ctgcgccata ctggttgcca cgctgttttt actcggctgg
     1921 ctgacggaga aggctttcga cttcgccggt aaatccgggc tgtatttcca gtgggggcag
     1981 aaaaaggctg agagtatcac tccggtgacg aacaccattc ccggtgaaga agacgacgat
     2041 gagccggttt ttagcgaaga tgctgtttca gaacatttgc gctttcgcta tgggcgtcgc
     2101 tggcagcgta aggtgcgact cctgctggtg atgggggatc cggatgtggt ccggaaagca
     2161 gccccgggcc tttgccatga cctctggcag gaaggcgacg gctacgtgct gatttatggc
     2221 ggagacgcgc agtcactccc ggacgagatc ttcctttcaa aactgaagcg tctgcgatcc
     2281 ggtcaaccgg ttgatggcat cattcaggtg atgagtattt cagccctcct gacggatggc
     2341 gaacgggatg ccctcctgcg ttaccgtcag aaagccgacc atcagttggg ctggcaggca
     2401 ccagtctggc tgtggctgac tgacaacgct accggtgccc ggcaggacgc gaaagcggcc
     2461 gcaacagggg tcatcttcgg accggaaggt accgtgaacg gtgcggacga agcgttttcc
     2521 acactggcgt tacacctgca aaaagcagga atggcgcaga tactgaatga acctgctgat
     2581 ttcggtctcc ttcagttgtc actcagactg cggcaggagc tgaaagccag cctggctgtt
     2641 ttgctcagtg gactgatgca ggggactgct gcctggcgtt tacgcggtgt catgttcagt
     2701 cctgaactga ccgttgccgg agcggtacca aacacatgcc ccgacacacc tgcctggaaa
     2761 gctattattg atgactgtga tgccgtcagc ggcaggaagc tgggctttaa ctggctgaag
     2821 atgctgcgat tgattctgct ttccctgatt gtcctgtggg gggccggtac actgctgtct
     2881 ctgctcgtta accgtactca gatttatcag gcgcaggaaa cggcgggaca ggcagcggat
     2941 acaacaaaac cactggctga acgcctgcgc agccagcttg tactccagca ggccattgcc
     3001 cgtctgcaac atcgtgaggc aaccggtgcg ccctggtaca cacgatttgg tcttaatcag
     3061 gacagcgaca cgctggccgc gctctggccg ctgtatgcga aaaataacgc acagctgatg
     3121 agggatgcca cagcagacag cctcaggcag cagctgaatg cctttgtgca actccctcct
     3181 gccagtgatg cccgtacgca gggcacacag catgcttacg acatactcaa aagttatctg
     3241 atgctggcgc gcccggacaa agcggacgcc ggctggctgg caaaaaatgc gctgaaagcc
     3301 ttaccgaaac gccggaatgt gccggatggc atatggcaga acgtcgcacc gaaactgctg
     3361 gggttttatg cgcaaaacct tccggctcat ccggaatgga aaattaagcc tgacgatgaa
     3421 ttaatcagta cggtccgtca gattctgctt aagcaaattg gccagcggaa tgctgagtcc
     3481 gggttatatc aggagatgct taagcgcgtg gccagtaact ggcctgattt aacacttgcg
     3541 gatatgaccg gtgacaccga tgcctcaacg gtattcagca ctgacgaagt cgttccggga
     3601 atgtttacgc gtcaggcctg ggaagagcag gtacaggatg cgattaacga agttgtcaga
     3661 acacgccgcg atgaaattga ctgggtactg acggataaaa cacatcagac agggagcgat
     3721 atctctccgg aggccctgaa gagccgcctg actgaacgct attttaccga ttttggtaat
     3781 gcctggctga acatgctcaa cagcatacag tggcatgaac ccacgtcact ttccggagcc
     3841 attgcccagt tgagtctgct cggtgacgtc cgccagtctc cgttagttgc actgatgaac
     3901 acgctgaact accaggggaa aacggggcag aagggggagg cactggctga ttcagtcgtg
     3961 gattcagcga aaaagctgct cgataagaat aaaaatggca ggcagtttat tgaacagaca
     4021 cagggccctg aaggtccgct cgatggggta tttggaccgc tgacagggct gatggagggg
     4081 aaagaggggg cgggtggcaa cggtaatctg agcttccggt cctggctcgc ccgtgtaacc
     4141 cagatgcggc tgaaacttca gcaggttacc agcgccccgg atccgcaggc gatgtcgcag
     4201 atgctggccc gggccgtgtt tcagggcaaa gccatcgatc tgacggacac ctgcgattac
     4261 ggcagcctgg ttgcagcaag tctggggcag gaatggagtg gatttggtca gtcgttgttt
     4321 gtgcaaccgc tggatctggc ctggcggcag gtgcttgtcc cggctgcggg cagcctcaat
     4381 gcccgctggc agacaaccat agtcgatcag tggaataagg cttttgcggg gcggtacccg
     4441 tttaaagcca ccggcagtga tgcctccctg ccgttactgg cacagttcct gcgtagcgac
     4501 tcgggtcgga tcacgacgtt cctgaaaact catcttggcg ggctcgtcca ccaggagggg
     4561 aatcactggg tcgctgagcc ttccgcaagc caggggatgg ttgtcagtcc ggatttcctc
     4621 cgggcgatta atcagctggc agagctgtca gatatcgtgt tctctcaggg cgatgcgggg
     4681 gttcacttcg aactgatggc gcgtccttca cgggatgttg ccagagtgca gcttaccctt
     4741 gatgaacaga atctggatta cttcaatcag atggaaagct ggcagagctt cacctggccg
     4801 gggaatactt actatccagg tgtgtcgctc agctggcgga gtgtaagcag tggtatgcaa
     4861 ctctacgcca gtcaccaggg gaactggggg tttattcgcc tgctggacga agccctgatc
     4921 acgccacttg atgccagccg cacacaactg gtgtggatca ctgccgatgg taatcctctg
     4981 aaattcgtca tgcgcagcga gctgggcgac ggtccgcttg ccttactgaa gctacagggg
     5041 tttacgctgc ccaccgccat ttttaacgtt gccgccggca cggaaggtgt tgagtaaaaa
     5101 tgaacagggg catttatccg acgccggcct ttactgaaga tctgggggct gatgggcaca
     5161 tttttcaccc tttcagagaa cggctccttc atgcatgccc gtcggcgttt tcgcccacat
     5221 gtcagaaaaa acaaccgata agctgacgga agggcaaacc cactcagcag gagaacgtat
     5281 gctgctgaaa tggaggccac cacaaatctt caacagtaag gacgacgctt ctccggaagc
     5341 ggtcgtgagg tttatgaaag agactgataa acaggaaaaa gaatgagttc accggaagcc
     5401 ctgttaacgg cctgcgccgc cagccaggaa gaaaggctga gcctcatttc ccgcgcaaat
     5461 gaggccctgt cgctctggga taactggctg catccattta cctccggatc tgaaaccggg
     5521 gacgatcctg catacgatga taattttcag ctgatgcgtg aagagatcaa taagatttca
     5581 ggtactgatt cagcgctgtt gtgcgaactg gctcagaagt gcctttgcga atgcgccaga
     5641 gatattcgtg tcgtgacctg gtacgtgctg gcccgtctca cccgggatgg tgaaagaggg
     5701 ttgtccgaag gcttattact tcttgttgcg atgctgaccc gctacggaca ggcatgtcat
     5761 ccccggcgtc ctgccgcccg gaaagctgcg ctggagtggc tgaacagtgc aaaagtgact
     5821 gatgcactgc tgttatggcc cggggtggac agcaatgagg ccggtctgac agcgggtgcc
     5881 ataagcctgc tgggatccgc cgtggcagac tggccggaag cggagaagcc gtcttttgcg
     5941 gggctttgtt ccgcgcttga gaaccgcctg gcacgttcag ggggcatgga ggcactggtc
     6001 ccccagaaca gcagtccccg ggagaccggg cgtgaatcag ctcccccgga ctcaccacaa
     6061 ctggcagcgg tgacctcggg gcgcgactta ctcgaccagg ccaggttact ttcccgctgg
     6121 ctgagcgggc agccgcaggg ctggctggct tctcaccggc tgatgaagac ggtgcggtgg
     6181 gatacggtcg atcagctccc ttcgctggac agtagcggac gcacccgcct ggtccccccg
     6241 aaaaccgggt atcgcgctca gcttaagcga ctctggcttc agaaaaactg ggttgagctg
     6301 attgaactgg ccagtcagat gtattgcgaa ggtgtgaatc atttctggct cgatcttcag
     6361 tggtatctct ggcagggact cagtcatgct ggtcagccct gggattcctg gtctgacgct
     6421 gtcctgtcag atctgcggct gttgcttaaa cgtctcccgg gccttgaagg actggcatgg
     6481 aatgacggta ctccctttgc tgacgaggtc accctcgcct ggatcgctga aaaagtgaat
     6541 gaagaagatg tgatgtacgg cgatgaagct gccattcctg ccgttgttaa tggccagact
     6601 gacgatgtgc tgatccttga gtctgaagcc atggaaaagg gagacacgga agggcctgaa
     6661 gcggcgcttg cctggctcca gtcccggccg gggatggata caccacgtca tcgctggctg
     6721 atacgcctgc tcatggcccg ggtggcagag cagtatggcc ggaacgatat ggcgcttcat
     6781 ctgcttggtg aacttattac ccgtgcgcca cagctgacgc tggaagactg ggagcccgct
     6841 ttgctgtttg aagtccgggc ccgacgcctg aagctgctac ggatgaaagc cggtcgcagc
     6901 gaatctgata aagcccgtct gatgcctgag atggatacgc tgctggcggg gctgatcgcc
     6961 attgacccgg cccgtgccat ggtgctctgt ggctgatgtt ctctttaatc attttcagga
     7021 atgccgtgca tggatgattt aaccctgcgc tattacgaag cagagatgcg ctatctgcgg
     7081 gaagccggta aagagtttgc ccgtgctcat ccggatcggg cagcaatgct gaatctggat
     7141 aagcctggcg cctgcgatcc cggtgtggaa cgtctgtttg aaggctttgc cttcctgatg
     7201 ggacgtttgc gtgaaaagct ggatgatgac ctgccggagc tcaccgaagg gctggtgagt
     7261 ctgctgtggc cgcactatat gcggactatc ccgtcgctgg ccatcgttga gttctcgcct
     7321 gactggcgta gtctgcgtca ggccgaaata ctggctgaag gcttctccgt tttgtcccgt
     7381 cctgtcgggc cgcataaaac cgcctgtcag taccggacga cccgcgatgt gttgctacaa
     7441 ccgctgcacc ttgcagatgc ccgtctccat actgaatccg atggccgctc tgccatccgc
     7501 ctgcgcttcg aatgccctga aaaagtggac tggagtaaag ccgggatcga caaagtcgcc
     7561 atttttctta atgcagaggc gccggtaagc gctgctctgc atctggcaat gacccggcgt
     7621 gtacatgcca tgtatgcgcg tcatgccggg acatataccg gacgtcatca gtttgacggc
     7681 tggtgcagac ccatgggctt tgacgacaat gactgcctgt ggaagaaagc cgatacagcc
     7741 ttcagtggtt accagttgct gctggagtat ttcagcttcc gtccgaagtt tatgtttgtt
     7801 gaactgcgcg gcctcgacac catcgggctc actgcggcca gtacctggtt tgaaatcgac
     7861 attgtgctca gtgaagcctg gtcatcctgt ttgccttttg agacagaaaa cttccgcctg
     7921 cattgtgcgc cggttatcaa tctctttacc ctggaagccg acccgctgct ccttaacccg
     7981 ctggataacg agtacctgct caggccgctg cgtcttcagg atggtcacac cgaaatttac
     8041 agcgtcgata acattcacgg ctcggtgaag aatggcagac acccttatgt cccgttcacc
     8101 agcttccggc accggggcgg tatgatgcgc cacgatgctc ctgaacgtta ttaccacacc
     8161 cgcgtgaaac ggggaccctc cggcctgtat gacacatggt tagtcctcgg gggacgttcg
     8221 ttcgaactgg agaagttgtc tgataaaccg gagtccctct cgatgcgaat caccgcgacc
     8281 aacgggcagc ttccgcgcag ggcgctggaa agtacgctcc tggacagggt ggtcaaaacc
     8341 ggcaaagtcc ccgtcagagt cctgaacctg accgcaccga cgatgccact gtatcccccg
     8401 gcaaatgacc ggtttcactg gcgggtgatg agtcacctcg gttcaaactt tctcagcatg
     8461 atggacaatc cggaggtgct gcgggggacg ctggcgctct atgactggac cgacgatgaa
     8521 atgaaccgcc gccggcttga agccatcgtt gccgtgaagc acaccctgat ccgccgtttt
     8581 gaaaagggct ttatgctccg tggcgtggac attgaggtca cgctgaatat ggataatttt
     8641 gccggcgagg gcgatgtgaa tctcttcggg gagatgctgc accgtttctt tgcgctctat
     8701 gctgatgttc accttttcaa ccagctcaca ctggtactgc aacccacagg gaaacgactg
     8761 agatggaaag agaatcacag ccggtacgta ccgggctgac gcaggccctg gcccttaaca
     8821 acgatatctg gcgggccagt ttttatcgtt tctgccagct gctggagcag gaaaatccgg
     8881 acgggccaaa actgggcacg accagccatc cggg
//
LOCUS       DAZGAP010000188         8848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGAP010000188 DAZGAP010000000
VERSION     DAZGAP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.189"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..346)
                     /locus_tag="ACHLAM_004283"
     CDS             complement(89..346)
                     /locus_tag="ACHLAM_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855731.1"
                     /translation="MDEERVFSLSYEQLTRLAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            943..1356
                     /locus_tag="ACHLAM_004284"
     CDS             943..1356
                     /locus_tag="ACHLAM_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855732.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            1358..2137
                     /locus_tag="ACHLAM_004285"
     CDS             1358..2137
                     /locus_tag="ACHLAM_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7855733.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            2317..2961
                     /locus_tag="ACHLAM_004286"
     CDS             2317..2961
                     /locus_tag="ACHLAM_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO7855734.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            3048..3356
                     /locus_tag="ACHLAM_004287"
     CDS             3048..3356
                     /locus_tag="ACHLAM_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO7855735.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            3769..4749
                     /locus_tag="ACHLAM_004288"
     CDS             3769..4749
                     /locus_tag="ACHLAM_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO7855736.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGADIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            4742..5158
                     /locus_tag="ACHLAM_004289"
     CDS             4742..5158
                     /locus_tag="ACHLAM_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7855737.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(5160..6434)
                     /locus_tag="ACHLAM_004290"
     CDS             complement(5160..6434)
                     /locus_tag="ACHLAM_004290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO7855738.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAIEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(6434..6871)
                     /gene="umuD"
                     /locus_tag="ACHLAM_004291"
     CDS             complement(6434..6871)
                     /gene="umuD"
                     /locus_tag="ACHLAM_004291"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7855739.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLHPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(6868..7116)
                     /locus_tag="ACHLAM_004292"
     CDS             complement(6868..7116)
                     /locus_tag="ACHLAM_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7855740.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSLSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            7534..8436
                     /locus_tag="ACHLAM_004293"
     CDS             7534..8436
                     /locus_tag="ACHLAM_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7855741.1"
                     /translation="MYFSAEPAQITEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            8433..8744
                     /locus_tag="ACHLAM_004294"
     CDS             8433..8744
                     /locus_tag="ACHLAM_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855742.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
BASE COUNT         2073 a         2120 c         2310 g         2345 t
ORIGIN      
        1 agcacggggc ctggccgttt ttcatcaagg gcagccagca caatcagcac tctccgctgc
       61 agtggcgacg gttggcggct ctgggtcatt acctgtcatc ctccggccag atgaggtttc
      121 tgaggcgctg aaagtcggcg tcaatgagct cctgtctctt tatggcattc attgaggcat
      181 agccatttat ggccagttca tgccagaaga tcagcacggc tccggctttg gctgactcgc
      241 agagataaat cgcgccctgg ctgtccagat tgcattcccg gatgcgtttt tccgcaagcc
      301 gggtcagttg ttcatagctg agtgaaaaca ccctttcttc atccatcctg cgcgctccat
      361 ctgataatgt tctgacaggt aaaaatcaaa ttctatatga tttttgtgtt attgatttct
      421 gataatatca gatattgttt cattacggat gattaaaatc atgtctgata tgaatttatg
      481 tgtgttgctt aattatcata tcgcctgccc gtttcccgcc gcgcagatcc ggtttccggc
      541 ttttctgccg cgcagatccg aaaaccctga gtcacggatc ttcctctccc tcacacaacg
      601 ccatctcctg tcagtacaac atgtcgtgct gcattcagct gctcatgaca ctatatattg
      661 tgttatttcc ctgacctgtg atgcgtcgcg caggggcgga aaacagcgat atgattattt
      721 ccccagcgtg gtacacttcc ggaaagttgt gatattcagg aaagtcggat ctgacggaaa
      781 cggctctccg gtaatttaac ggcgtggtta tatggatgct tgttatcatg gtgatgatga
      841 taacggcatg atgttatcag acggcgtgac ggtaagggca gtgatgatgg atgacgttat
      901 cgcatgaccg tccctgcccg gaaaagaaaa aaggagtcac ccatgttttt tattgagaat
      961 gaaggtcagg ctgtcgccag aacggattac tggcagtctg tacaggcgca ggccggatat
     1021 gtctacctca gctggaatgc cggcgcagcc aggctgcttg tcccggatgc ggcaaaacat
     1081 ttactcaggg agatgcgggg ggctgagtac gtcatcatca gtaagggaac gctggatggc
     1141 cgcgatgcgc tggaactgat atttgaagac ggcagcgatg cgccgtttgt gatccacatg
     1201 ctgagtgagc agtgcgatcg cctgctcccc gaaaacaacc agggaggggg ttttgttgtc
     1261 accgtctgga cgcgtggcgg taaccagctc cgttatccgg gaaaataccg ggttgtggaa
     1321 aacctgcccg acgtttcccc gtggagtgag cactgatatg cagcacctgc cggcaccgat
     1381 ccaccatgcc cgggatgccg ctcagcttcc tgttgccatc gattatccgg cagcgctggc
     1441 actccgccag atgtcgatgg ttcatgatga actgccaaaa tacctgctgg cccctgaagt
     1501 gagcgccctg ctccattacg tgccggatct gcgccgcaag atgctgctgg ccacactgtg
     1561 gaacaccggt gcgcgcatta atgaagcact ggcgctgacg cggggggatt tttcgcttgc
     1621 gcctccgtat ccgtttgtgc agcttgcgac cctgaaacaa cggacggaaa aagccgccag
     1681 gacggcagga agaatgcccg ccggtcagca gactcaccgg ctggttccgc tctccgacgc
     1741 ctggtacgtc agccagctgc agacgatggt ggccacactg aaaatcccca tggaacggcg
     1801 taataaacga acaggcagga cagagaaagc gcggatctgg gaagtgacgg acagaacggt
     1861 caggacctgg attggggagg cggttgccgc cgctgctgct gacggtgtga cgttctctgt
     1921 cccggtcacg ccacatacgt tccgccattc ctatgcgatg cacatgctgt atgccggtat
     1981 accgctgaag gtcctgcaga gcctgatggg gcataagtcc atcagctcaa cagaggtcta
     2041 cacgaaggtg tttgcactgg atgtggctgc acggcaccgg gtgcagtttt cgatgcctga
     2101 gtccgatgcg gtgtctatgc tcaaaagaat tccttaagct ctgacttttt taaatccctt
     2161 tcggatatct catggatata ttatcggtat ttcatattct gttgacatgc aggaccggta
     2221 tagagtaaaa aaacatctat tgagtacctc ttggatatcc gaaaggtgtc ttagggggtc
     2281 tttatgggta ttacatgtgg attctggaga tacactatgc ctgttattgc taatacgcat
     2341 cccaaaggtg gtgtgggtaa gacaacttca tccgtaaaca ttgttggtga aatgaagtct
     2401 gatacagttg atctggatac tcataccggg ctttctatca ttctggggct gagacctgaa
     2461 ggaaaagaaa tttccgtgaa agtacccaaa acagtggatg agttaatcga aattatgact
     2521 ccctacaaga acagcgataa gacgttactt attgactgtg ggggatttga ctcagatctt
     2581 acccgtactg cgattgcctt tgcagactgc gttattgtcc cttcaaaaga ctccctgact
     2641 gaacgtattg gtctgatgca ttttgatgga gtactggacg aaatcagctc tatcatgggg
     2701 accgatataa ctgctcacct gtatctctgt aaagttaatc ctaacaagaa gaaattccct
     2761 aagcttgatg caatcctgcc atcgttcaaa catctgaagc tgatgaagag ccgcatttct
     2821 gctcgtgcag aatttgagga tgtcattgag actgggatgg gaatcactga gtctgttcat
     2881 gggcgctatt cggcaggagg taaggaagtt attgccctga tcgaagaaat caatcatctg
     2941 attgaaaata acaaacagta aggtgtatct attgggtatt catatagtat cctttgggct
     3001 tttaatggat atcttttaag tatctaacaa acatcctggg ttatctcatg agcaaagcga
     3061 aatttagtgc acaggctgaa aaactgaaaa acctggtgga ttctgccaga ttacaaactt
     3121 cttcttctgc cgtaacagca acagccccgt ctcagccaaa tgaaggtgca gaggaagttt
     3181 caggtcgtcc cgtggctgcc aggacgaagg ctaagaatct taaggccata ccgctgtcgt
     3241 attttgaagc tcatgcggaa cttaaagcga ccagtaaaac atctctggat tttagctcat
     3301 atattattga ggctataaga gagaagcttg aacgtgatgg tgcaataggt caataaacta
     3361 tcggatacct taagggtatc tgatgtgtgt cttttaaatg cgttatgggg attcttcacg
     3421 tacctaaaga gtccccatta gacatctttc tgtttatggt gacatgattc gtcaccgctt
     3481 tcctttcctc tcagtttcac catttcattc ctttccctta acctgaacca tgatgtccac
     3541 gatgttttta tattgcattt atgtatcaca tataaccttc ataatgtttt tgtatctatg
     3601 atttcctctg gtcttaaagt tgtgcgtaca atgtacatgc aatttatcgt gtttttatgt
     3661 tgcatttgtc ctttcagaaa gaccgttttt tcattataat tgaatataca ttgcatgtat
     3721 gaagcatgtt tgttttttct atttgtttat gagcaacgga gactggttat gaaaatcttt
     3781 attgatgatg gttctaccaa cattaaactt gcctggttgg aggatggtga cgtgaaaacg
     3841 ttaatcagcc ctaacagctt taaaccggaa tggtctttta gtttactgga tgatgctgct
     3901 ccggctaatt acgagatcga cggcgagaag ttttcatttg atccattaag tgctgatgct
     3961 gtcgtaacga ctgaaacacg ctatcagtac agtgatgtga atgttgtggc cattcagcat
     4021 gcgctacagc agaccggcct gaaagcgcag cctgttgatg tcatcgtcac attgccgatc
     4081 tctgaatacc ttgacgctaa caatcagaaa aataagcaaa acattgaaag aaagaaaaaa
     4141 aatgtgatgc gtgaagtacg ggtgcagggg agtgatgcat ttgttattcg cagtgtttcc
     4201 gttcttcctg aaagtattcc cgctggtttc agtgttcttg cggggcttga ggatgatgaa
     4261 tcccttttga tcgtagatct cggtggtaca actcttgatg tgtcgcatgt tcgtagcaaa
     4321 atgacaggga ttacaaaaac ctggtgtgat ccgaatatcg gcgtgtccct gattacatcc
     4381 ggggttaagg aacagatggc tgtacatgct aatacaagag tgagctcatt ccaggctgac
     4441 aatatcattg tgcaccgtaa tgaacctgac tacctttccc gtcgcattta taatgctgaa
     4501 cagcgtgagt ctatcattaa tgttatcaat gaacggcaga agttgcttat taaacgagtt
     4561 aatgatgtca tcagccgttt caccgactat acacatgtaa tgtgtgttgg tggtggggct
     4621 gacattgtgg ccgaggctgt gaagaacctg actaaggttc ctgatgagcg cttttatctg
     4681 agctcatccc cgcagtttga tttagtcatg ggaatgataa aaatgaaagg tggtgtgact
     4741 aatgagtgat gaaaataaat ccagacgctg ctcatttgaa cttttcccgg atgaacggac
     4801 aggagataag atagcagatg agctcattgc aaatgaaaaa ctgaaggagc ggggacgttt
     4861 catgcgcgcg atgttagtta ctggtgcggc gtttgcagct atagataaac gtcttccttt
     4921 gctgatatcg gagttactga cagaaaatac gactctggat gatattaata aggtgatttc
     4981 cagcgtaata ccaggtgctt tttcggttga aaagaaattg cttgaattac tggaaaaaca
     5041 atctggtctc catacgtctg tggattgcag tactcctctt acagagcaga gtctgtcgag
     5101 aaatgacggt gaagaccaga ccagaagaaa cgctgaaaat atgttcggag atgactgaac
     5161 taacccagcc tggcaagggg aatatctcgc catttagttg tataacactg actcaacatt
     5221 tcccgtttca tttgccattc aggggcggtt cctcgccccg caaaccacac tttccctttt
     5281 cccgactggt tcagttcatc gagtgttttc attaactttt cactgttttt acggggctgg
     5341 atttcatcaa ataatcccgg ctgcgctata cccgatggtg tgaaatcagc cagcatgact
     5401 cctgccttca tatagcggta cccttcacgc cagacatggt ttaaggctct gcatgcggcg
     5461 gcaataatgt cccggctgtc ctgtgtgggc aatggaagct tttccacagc ggcattgctg
     5521 taacagggtt cttttactgc aaagggggat gtccgtacaa atgtcgtcac ctgccggcaa
     5581 tactgacgct ccccacgtag tttctctgcg gcccgctctg catactgaac aacagcctgg
     5641 tgcatggcat ctttgtctgt gattcgttca ccaaaactgc gactacagac aatctgctgt
     5701 tttgccggtg gtgcttcttc cagggatatg caggactcgc cgttgagttc gcgtaccgta
     5761 cgctcaagaa tgacgctgaa gtttttccgg atgaatgccg tgttagcctg cgccagctgc
     5821 agtgctgtgt taatacccag cgcattcagc ttttccgtca gtctgcgtcc tactccccag
     5881 acctcaccaa ctggctgcag ccccagtagc ttcaaggtcc gattacggtt ttctgccgtc
     5941 agcgcgacca ctccggaaaa ctgtggccat tgctttgttg cccactgtgc acttttagcc
     6001 agcgtttttg taggcgcaat gcccaccccc atggtgagtc ctgtccagct ctttacctgt
     6061 tccctgagct gatgaccaaa aaactccgga gagatgcaat gatttatccc ccgcaaatca
     6121 ataaacattt catcaattga gtagggctca attgcgggag aaagcgactc cagaacagcc
     6181 atgacccgtt ggctcatgct gtggtacagc gcataattgc tggaaaatac atgtattttc
     6241 ttctccaggc gcatctgtct cacctgaaac cagggctgcc ccattctgat gccaagggct
     6301 tttgcctccg ggctgcgcgc gatcacacag ccatcgttat tgctgagtac gatgaccggt
     6361 tcgttgcgaa ggtccgggcg gaaaactttt tcacatgagg cgtagaaact gttgatatca
     6421 gccagtgcaa acatcagcgt aactcccgcg tcctgtgtat cacgtgagtg acaacaccaa
     6481 aaatacagat gttttccgga tacagtgtgc ggaactccgg gctgtctgaa accggctcca
     6541 gtgccgggcg tgggtgcaac agcagtcgtt tgacggtgaa ctccccgtcg atctcagcga
     6601 taacgatgtc cccgtgttgt ggtttttcgg ccctgtccac taccagcaga tcaccattct
     6661 gcacgccagc ctggttcatc gattcaccgc tggcgcgcag aaagaaggtg gctgcaggtc
     6721 tgctgatgca atagctgttc agatccagtt cctgctcagc ataatcagtg gcaggagaag
     6781 gaaaaccggc ctggcaacga tcggcaaaca acggtcgcac gtaactgtca tcgcctgaag
     6841 gggcagccgg acggtgataa acggtactca tgctgcaggc agccagctgt cgtcctgcca
     6901 gatttcttcc agcagagaca ggatacgttc tttatccttg tcgttgcggg ctcctgtgac
     6961 agatagagac ggggcgctgc cctggtctat acgcagccag atttccgggt agtgtggttt
     7021 cagacgttgt tcgatttcat tctgtagcgc ctgaagtgtg cctgatttga ggttttttgt
     7081 gctctggcgg tcgaaaagaa tttcaatgcg gatcatgttc tcatctccct tttcttttgc
     7141 tgtatatgta tacagtattc taataactga atccgcacac agtcaaatgt cattgttctg
     7201 actaaaaagc gatcaattct ctgagggggt ggctgtatgc ctgctgtgga gtgctgtatc
     7261 cagcttatcc acagcatttt gtgcacggtt ctgtggacaa aatacctggt tacccaggcc
     7321 gtgccggcac gttaaccggg ctgcatccga tgcaagtgtg tcgctgtcgc cggcctcctc
     7381 acccggtcac gtttcgtcgt tcctcctcca cgcgctgcgg cttcggggcc gcacctgcat
     7441 tcgtatgcgg tcgcccggtt acaggtgcgg cacggcctga tggaggccgc atgtgagagg
     7501 agaattccta tgccaaactg gtgctcgaat cgtatgtatt tttctgccga accggcacag
     7561 attactgaga ttaaacgact ggccagcggt gcagtcacac cgctttatcg ccgcgccaca
     7621 aatgaaggta ttcagctgtt tctggccgga agtgccggac ttctgcagac cactgaagat
     7681 gtgcagtttg aaccgtgccc cggactgacg gctgccggac gtggtgttgt atcgccggag
     7741 aatatcgcgt tcacccgctg gctgacacac ctgcaggacg gtgtactgct ggatgagcaa
     7801 aactgcctga tgctgcatga actctggctg cagagcggta ctggacagcg tcgctgggaa
     7861 ggattaccgg acgatgtcag ggataccatc accgcacttt tcaccgcaaa aagaggtgac
     7921 tggtgtggct tctggagtaa cgaggatgta tcggtgtggt ggaaccgtct gtgtgacaac
     7981 gtactgccgg aaaaaaccat gccgtttgac ctgctgacgg ttctgccgac ccgcctggat
     8041 gttgaagtga atggctttaa cggtggtgtt ctgaacggtg ttccttctgc atatcactgg
     8101 tatacggaac agtatggcgt gaagtggcct tgtgggtatg aggtgaatat cagcagtcag
     8161 ggagacaact tcattaaggt ggatttcgac acgccgtggt gtcagccgga aagcgacgtt
     8221 attgcagaat taagccgccg tttcagctgc acgctggagc actggtatgc cgaacagggc
     8281 tgtgatttct gtggctggca gttgtatgag cgcggagagc tcgttgatgt gctgtggggg
     8341 gaactggaat ggtcttcccc gacagatgac gatgagctgc cggaagtcac cggacctgcg
     8401 tggatagtcg acaaggtggc gcattatggc ggatgaagta tgacggagac gggcgggcaa
     8461 ccgcccgttt cttttccggt aaaggatgtc gccgtgctcc tctttttact gcgccggctg
     8521 acgcggcgcg gcataaacgc tgcctgtggt cagtgtcccg cgtccggcgg gcacgggacg
     8581 ggcgctttta ccgctcccgg ctggcgtcgg tgacagtgta cgccagcccg tcgccctttt
     8641 ctgatgaacg cccttcaagc cgctttcgcg gcataacctt gccgtcaaaa agacgacggc
     8701 tgcggtattc cacggtcggc ctgacccgtt accggacgcg gtgaacagcc cacaggcagc
     8761 ggggaacggg caccacaggg gtgccctccc ggagccctct gtaagagaag gagttttgta
     8821 tgtcccgttt tgtccttggt aactgcat
//
LOCUS       DAZGAP010000189         8781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGAP010000189 DAZGAP010000000
VERSION     DAZGAP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.190"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..297
                     /locus_tag="ACHLAM_004295"
     CDS             <1..297
                     /locus_tag="ACHLAM_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO7855743.1"
                     /translation="IISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQYR
                     TASILKNRRVVFNVKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            300..659
                     /locus_tag="ACHLAM_004296"
     CDS             300..659
                     /locus_tag="ACHLAM_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7855744.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(706..1098)
                     /locus_tag="ACHLAM_004297"
     CDS             complement(706..1098)
                     /locus_tag="ACHLAM_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7855745.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1225..1485
                     /locus_tag="ACHLAM_004298"
     CDS             1225..1485
                     /locus_tag="ACHLAM_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7855746.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1482..1919
                     /locus_tag="ACHLAM_004299"
     CDS             1482..1919
                     /locus_tag="ACHLAM_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7855747.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2006..3016
                     /locus_tag="ACHLAM_004300"
     CDS             2006..3016
                     /locus_tag="ACHLAM_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO7855748.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEVYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSELHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAIQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWR
                     DD"
     gene            3009..3470
                     /locus_tag="ACHLAM_004301"
     CDS             3009..3470
                     /locus_tag="ACHLAM_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7855749.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISGSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            3504..4220
                     /locus_tag="ACHLAM_004302"
     CDS             3504..4220
                     /locus_tag="ACHLAM_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7855750.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLIKAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPVADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEDIKTETVADIVQSLPSFTETRADDLVLPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDILRDITATRELLR"
     gene            4253..4534
                     /locus_tag="ACHLAM_004303"
     CDS             4253..4534
                     /locus_tag="ACHLAM_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7855751.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            4531..4836
                     /locus_tag="ACHLAM_004304"
     CDS             4531..4836
                     /locus_tag="ACHLAM_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO7855752.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVVPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            4823..5425
                     /locus_tag="ACHLAM_004305"
     CDS             4823..5425
                     /locus_tag="ACHLAM_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7855753.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLADVRAEVFND
                     LCAAFVRHAKVAGLDDADTVTFKEATEALLHCAEQLRAPE"
     gene            5521..5703
                     /locus_tag="ACHLAM_004306"
     CDS             5521..5703
                     /locus_tag="ACHLAM_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855754.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            5696..5881
                     /locus_tag="ACHLAM_004307"
     CDS             5696..5881
                     /locus_tag="ACHLAM_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855755.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGEDDED"
     gene            5987..6190
                     /locus_tag="ACHLAM_004308"
     CDS             5987..6190
                     /locus_tag="ACHLAM_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855756.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVDKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            6183..6422
                     /locus_tag="ACHLAM_004309"
     CDS             6183..6422
                     /locus_tag="ACHLAM_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855757.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRGMQLQEAKARLEKKIALYINDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIHKGGNQ"
     gene            6419..6763
                     /locus_tag="ACHLAM_004310"
     CDS             6419..6763
                     /locus_tag="ACHLAM_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7855758.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALSAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            6765..7208
                     /locus_tag="ACHLAM_004311"
     CDS             6765..7208
                     /locus_tag="ACHLAM_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855759.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VVYMWDSERKDIDAPGYYRTEHLVFAESSVKQWGGRVVPLYTAPPAPIVPAVLERLRA
                     IVADPRALPRRKEWVSGQQYSYVLLENVEAMVDEACRAAMLQGDK"
     gene            7208..7351
                     /locus_tag="ACHLAM_004312"
                     /pseudo
     CDS             7208..7351
                     /locus_tag="ACHLAM_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7379..7909
                     /locus_tag="ACHLAM_004313"
     CDS             7379..7909
                     /locus_tag="ACHLAM_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855760.1"
                     /translation="MEEAKELHEDLVRSHISKALSGEKMKKKDLDADLCWIHGVIVQA
                     SWFVKASLEQNALTGNSQVIPDDGREQFETLIRFHADDKNHETLLLRANEGMNYRDPN
                     VDLAWIFWKSSREHILPGKSDNNPALGDQVSELTMLVKRLASSLKSVNKSSKLPDKAM
                     DYLKRNGLISAEDILR"
     gene            8207..8362
                     /locus_tag="ACHLAM_004314"
     CDS             8207..8362
                     /locus_tag="ACHLAM_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7855761.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(8469..>8781)
                     /locus_tag="ACHLAM_004315"
     CDS             complement(8469..>8781)
                     /locus_tag="ACHLAM_004315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855762.1"
                     /translation="GCVNPYNYREEQNVIISFKTNSPPQEIQECILSEWQRTPLLATI
                     TSQKIGKYYSVLAVGDNADIYKLADGSTIIDFYSLRGVLDPTNGKSKRIQGIKSCIST
                     K"
BASE COUNT         2462 a         1849 c         2424 g         2046 t
ORIGIN      
        1 attatctcag ttaaaacact cagggatttt tgggcggaga atcctgatgc agaacaaccg
       61 ctaagggcat gggtggatga ggcggcaaaa gctgactgga aaagcccagc agacattaag
      121 gcacaatacc gaacggccag tatattgaaa aaccggcgcg tggtattcaa cgtaaaaggt
      181 aatcattacc gtttaattgt tgcaattgcg tatcagcgag gatgggtatt tgttaaattc
      241 atcggaaacc acaaggaata cgatgctatt gatgctgata ccatcgaact ggagtaagca
      301 tgaacatcaa acctattcgt acagagcaag attatgaagc cgcgttgcgt gctgttgaac
      361 ccatgttcga caatgagccc gaaattgaca cgcctgaggg ggattttttt gaggtgatgt
      421 gtttgctaat agaggaatat gaaaaaaaac attatcccat tgagccacca tccccaattg
      481 aagctataag atttcgcatg gaacagcagg ggctgactgt gaaagatttg gaacccgcaa
      541 ttgggaaaaa aaatcgggtt tatgaggtgt tgaatggtac cagaagcctt acgttaccaa
      601 tgattcgccg tcttcataat caatttggta tccccctgga aagcctggtt ggattataaa
      661 atctgctagt catttgcctg atgctcgttc cagaaaagga atgcatcagg cagttttgtt
      721 tttctgccgc agtaactctt caagtttccg tttatagaaa tcgcgttttt gctccatatc
      781 acgaatgatc tgctctgcgt cgctttgagg taactcatct aaaagcgata tgatttttcg
      841 ttgttgttct gtaagttgcg gttggttgtc attactggat acagccattt tatcgccgag
      901 agtttcttct tccataaaga accaatggac gggatgttgt gagagctctg ctaatttttc
      961 cagtttatcc attcttggca tcacgccttt caaccaacct tgcacggatt ggggttttac
     1021 accaagacgt cttcccagct ctgactggtt tatattcaat tcctgcaaca cctgctgaag
     1081 gcgttttaca aagatcatca ccacccctcg taaactagtt ccgcgatcct acagaaaaaa
     1141 ttgataagtg gcattacaaa tagaagttga aatttaaatt taaatttgta atcatcggtg
     1201 tcatcgtaaa gttcggaggg aaacatgcaa aaaagtactc aagtgaaaat cctgtcaata
     1261 atgagccaat cagaattagg gcgtcgtctt ggtaaaacac cgcaaaccat aagtgggtgg
     1321 tttaaaaaac gagtgccagc ggaggaggtt ataccagcat gtgaggcgct tgactgggga
     1381 gtaactccgc atgaattgcg ccctgataaa taccctaatc caaccgatgg tttacctgtt
     1441 gagtatcagg ctaacgcaca agcagcggcg ggagttgatt catgaaaatc aagcatgagc
     1501 acatccgcat ggcgatgaat gcgtggctgc tttatccgag ggtagggcgc aaaaaaatcg
     1561 ctgatgatat agcgacagca tattttgagc ttgaaatgac ttatccacca atgcatgaca
     1621 cctctacgac agagggtatt ggattgaata tacaaaatat ttttcgctgg cttgaaaagg
     1681 atacgcctga tgctgttgaa aaaattcagg cactaattcc ggctatcctg actgttcttc
     1741 cgcgtgaact gcgttatcac ctcagtattt ttgacactgt tgagcgccgt gcattactgg
     1801 cggcgcagga agcgttgagc acggcaattg atgcacatga tgatgctgtc caggccgttt
     1861 accggaaagc gtatttcagc gacggcgggt catccggcga gtctgttgtg gtgcattgat
     1921 atttatgcca gccccctgct gattctgttg attggggaat cacagaatat caccagagga
     1981 tggttcgtca caagatgagg caattatggc cgcattacca tacatgcaac tgtacatagc
     2041 tgattacctg gctgacacca tgcatttgtc cgcagaggag cacggtgcgt atttgttgct
     2101 gatgttcaat tactggcaaa cagggaaacc aatacctaaa aacaggctgg caaaaatttc
     2161 ccgtctgact aacgagcgat gggctgatgt ggaaccatcc ttgcgggagt ttttttgcga
     2221 taacggcgat gaatgggtgc atcttcggat tgaggaagat ctggcatcag tcagggaaaa
     2281 attgaccaaa aaatcagccg ccggaaaagc atctgttcag gccagaagaa gcagaaagga
     2341 agtatatgtt caaacaaaac aagagagaga tttaacaggt gttcaaacaa atgttggtgt
     2401 tgtgtttgaa catgacgcaa acacaaaagc aactaataaa gatacagatc taaaagaatt
     2461 aaaccccaca cataacgtgc gtgaacgcga gagtattccg accagtgagt tgcatggtgc
     2521 gccgttgcag acagccgaac ctgaatacct ggacggcctg agcgaaccga tcgggaaatt
     2581 ttcgatgacc actgtctggc agccgtcgcc ggattttcga caacgggcag cagtgtgggg
     2641 tatggctctg cctgagccgg aatttacacc tgctgagctt gccgcattcc gggattactg
     2701 gatggcggag gggaaggttt tcacacaggt tcagtgggag cagaaatttg cccgccacgt
     2761 gcagcacgtc agggcacagg taaaaccagt cagcaagggg gtaagccatg cagcatcagg
     2821 tggcacggca tcacgggcaa ttcaggaaat ccgggcagca cgcgaacagt gggaacgaga
     2881 caacggattt atcagcaacg gaaacggcct ggaagctgtg ggagcttatg ggggaggtgt
     2941 attcgaaccg ctggactcag aagaacgggg ccgcaccttc gaagctctgg attgcccaga
     3001 ttggcgcgat gactgaacag caaatccggc tggtctgccg tcagtgcatg gaccgctgcc
     3061 gggcgggtga aacgtggccc ccggacctgg ctgagtttgt tgcgctgatt tcggggagtg
     3121 gggcaaatcc atttggtctt acggtggatg ccgtgatgga agagtaccgg cgctggcgca
     3181 atgaatcctg gcgatacgac gggagtgata aatacccgtg gccacagcct gtgctgtacc
     3241 acatctgcct cgaaatgcgt accagaggga ttgagcgcca gatgacgcag ggtgagttaa
     3301 aacgacttgc ggaacggcaa ctgacgaaat gggcaaagca tgttggtaac gggatgagtg
     3361 ttccgccagt gcgacgacaa ctggaagggg cgaaacaccc gcaagggcca acgccaattg
     3421 aacggctgaa acaggaatac gaacgccgga aggcagctgg ttttatttga atctgagaaa
     3481 cgattttgtc ggaggaaatt ttaatggaaa ccgtatttga cgcactgaaa gcactgaaaa
     3541 aagcctcttc acaggtagtg gcatcgcgcc ttggaatcag ccgcgaagat gcggtcaacg
     3601 aactgtggaa actgaagcgc cgcggtgaag cggataacaa gggttcgatg tggtggctga
     3661 ttcaggctgg tgaaagtgaa ccagtgtcac cggtaccgaa agtgacagcg caaatgctga
     3721 ttaaggcgat tgaacaacat ggcccacaaa cggcggatga gctggcactg atgttcggga
     3781 ttacctcccg ccgggcgaat tcatcgctgg ccatggcaat cagcaaaggg cgtctgattc
     3841 gcgtgaatca gggcggtaaa tttcgttact gcataccggt cgctgattta ccggcagagc
     3901 cggaagctgc atccgtagcg gaaacggatg gtaaagcctt tcctcagcca gcaggtgttg
     3961 cgttaccagt ccgggaagcg gaaacacagg aagatattaa aacagaaact gtggctgata
     4021 ttgtgcaatc gttgccatcg tttactgaaa cgcgagcgga tgacctggtt ttaccatcgc
     4081 tgcatgtggc taaccgcgag ctgcgccggg caaaaggtca ggttcagaag tgggagcgag
     4141 tctgtgccgc gctgcgggaa ctgaacaaac acagggatat tctccgggat attaccgcca
     4201 ccagagagct gctgcggtga gtggctggaa gaagtggcgc tgggcggaaa ttatgatact
     4261 ccggcagtgt gcgggaacga tgacagtcga aagcatcggc agtctgattg gccgtagtgg
     4321 ggctgccgtc aggacgaaag cgcgggaact gggtatctgc atgatgttac ggggtgatta
     4381 tcaccagtca gccaaatgtt cacagcgtga tattgagctg gcgcggcaac tgcatcagcg
     4441 tggcgtaccc cgacgggaaa ttgccgaaaa gtttgggatg aagttgggcg cagtgaataa
     4501 ctacgtttat ttcgacagga gggttcaggc gtgagggtga gaatttatat cgccggtccg
     4561 atgacgggat atgaaaattt caaccgtgag gcgtttcaca aggcggaaga agcactgaaa
     4621 caggaagggc ataccgtatt aaatcccgca gtagttccgg acgggctgac tcagccacat
     4681 tacatggata tttgtatggc aatgattcgt tgtgtggatg cggtttatat gctgaaaggc
     4741 tggcagcggt cggcaggcgc taaggcagaa ctggcactgg cggagaaact ggggcatgca
     4801 gttattttcc aggaggcaac cagtgagaaa aattaattat cagacgctac gtgagatagc
     4861 aaaactggca acccagggcg aatgggtcgc attcatttcg ccgggcactg atacatatgc
     4921 ggtgcatacg ccgggggatg aacgttgcgg agacattatc aaatggcctg gctttgatga
     4981 ccagaaaaat gcagagaaca acgcagaatt tattgcagct ttcaaccctg aagtagtaca
     5041 ggcactgctg gatgaacggg aagcccaaag caaacgtatt gccgagctgg aagctaatct
     5101 tgtggcgctg gctgcggaga atgcacttgc tcgtaaagca gttcaggcat tctgcgatgt
     5161 tgttggcgac aacaccgagg ttatttgcga ggaggttggg agagatggcg ttctggttat
     5221 tttggaggcc atgaaggcaa caggaaatat gccagccacc gatgctttcc tggctgatgt
     5281 acgagcggag gtgtttaacg acctttgcgc ggcgtttgtc aggcacgcaa aagttgcagg
     5341 actggatgat gccgataccg taacgtttaa ggaagcgaca gaagcattgc tgcattgtgc
     5401 agaacaactt cgcgcacctg aataattaaa tttagcactg taaataaaat ttaatcctta
     5461 accggaggga ttcctgcacc ctcagaacat caggagaccg cccggcaggg cggtagtgaa
     5521 atgcgaaagt tcaaaataat tattgaaacg ggaatagccg gtggagattt tgaggatgta
     5581 ttcgaagtgg acgatgacgc aacacctgat gaaattcatg acgaagcaaa agaaattttc
     5641 tttaactact gcaattactc atatcacgaa ataaaagacg aagaggaaga acaaaatggc
     5701 tgattttggt tcaactaaat ataacgccag ttttgaagaa tggcatgaac tgttaatgga
     5761 ctatgcagag ttacgcggtg gaagtgccgc cgatgctgaa gcatggcgtg atgactatga
     5821 agcaggaaaa actccggttg aagcttattg tgatgagtgg ggtgaggacg atgaagatta
     5881 actatcaggc gatggtgcag gccaggcgat tcgataccgg ctcgtgttca gagtcatgtg
     5941 aaatctgggg tgaacaattg gaattgaaat tcgaagaggt ggtggcatga caatcgtaaa
     6001 aacccatacc ggcatcgtga tcaccaaaga cggtccgcag gtaaaaaaac tgcaccagac
     6061 ggagcggatg tgggtcgtcg acaaaaacga gttttaccac aaagaaaccg gacgccgcca
     6121 ctttgcagaa aatacgcgcc gccgactact gatcgacacc atcaagcctg tcgaggtgaa
     6181 gcatgtttaa acagaacgaa aaatctatcg ctcaaattgc tgagtatatc ccgcgtgcgt
     6241 gccggggtat gcagttgcag gaagccaaag cgcgcctgga gaaaaaaatt gcgctctata
     6301 tcaatgacgg ctgtgatgct gccgttctta acgcggcgtt cgcaccagct cttaacagtc
     6361 atacgcggga gtcttttttt tcgtgcatcg cagcgcagat ccacaaagga ggcaaccagt
     6421 gagcaagatt gactatcagg cattgcgtga ggcagcacaa aactatcggt cgatgctggc
     6481 gtggtatcag gagaaaccag acagtccaaa cgctgagcag gattgtgatg cagctttgtc
     6541 tgcgtttaag cgagaaatcc gtcatcgtga agtggatatt atcgctgatt tgctggatga
     6601 actggaggaa gcaaaacagc gtatcgacga gcaggagtcc cgcacagtga agttaccaga
     6661 accattcaag ctggctaaat catcaagtgg attaacgtac tactacgctg atgaggtcaa
     6721 tgcagcactg actgctgctg gtattcgcat agaaggagag tgagatgacc actattacca
     6781 aagagcgact gcaatggctg gctaacattt ctggccgcga tgacattgac gatatagacg
     6841 gcggtgaaat tcgtgagctg gcgcttatcg cactggcatc gctggaagca gaaccggtgg
     6901 tttacatgtg ggatagcgaa agaaaagata ttgatgctcc tgggtattat cggactgagc
     6961 atttagtgtt cgctgagagt agtgttaagc aatggggagg gcgagttgtt ccactttaca
     7021 ccgccccgcc agcgccgata gtgccagctg ttctggaacg tttgcgagct attgtagcgg
     7081 acccacgcgc attacctcgc agaaaagaat gggttagcgg gcagcagtac agttacgtac
     7141 ttctcgaaaa cgtagaagct atggttgatg aagcctgccg cgcagccatg cttcagggag
     7201 ataaataatg attaatcgaa ccaaactgga gcacattctc gaatatgcca ggcagcagaa
     7261 atgtattggg caactttgta aaattccacc aggggatatg gttgaaatca tggagattgc
     7321 catgcgcaag gctggcaact ctccgataac tcaggctagt ctgcctggcg gtttcaccat
     7381 tgaggaggcg aaggagttac atgaagacct ggtgcgcagc cacataagca aggctttaag
     7441 tggcgaaaag atgaagaaga aggatctcga tgctgatttg tgctggatcc atggggttat
     7501 agtccaggca tcctggtttg taaaagcatc actggagcag aatgcgctaa cgggtaactc
     7561 tcaggtaatc cctgatgacg gtcgcgaaca gtttgaaaca cttatcaggt tccatgcaga
     7621 tgataaaaac catgaaacat tattgcttcg tgctaacgaa gggatgaatt accgggatcc
     7681 aaatgtggat ttggcgtgga tattctggaa atccagtcga gagcacatat tgccgggaaa
     7741 aagcgacaat aatccagcat taggcgatca ggtgagtgaa ttaactatgt tggtcaagcg
     7801 attagccagt tcattaaaaa gcgtcaataa atcaagcaaa ctaccggata aagcgatgga
     7861 ctacctgaag cgaaacggac tgataagcgc ggaggatatt ttacgatgac ctggcctgag
     7921 gcattcgtgg cggtaggaat tgcaatggcg gtggcgctgg tgctggtggt gtattcgatt
     7981 tgccgctggg gataaaaacg gtttgcgggg aaagaagagt taagtagaag tgctgcgggt
     8041 gcttgaggct gtctgcctcg ggcatgccgc cgtaaggcag acagagaaaa gccccagtta
     8101 acattacgcg tcttgcagga cgcttaacat taatctgagg cccaatctat gcttcacaaa
     8161 cgtaggttag cctcttacgg accgaaaggt caaggagaag cagactatga agcagcaaaa
     8221 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     8281 gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggttg ctgtcttcgt
     8341 agactacgaa tctgagaagt aagagtgacc aggcgaggga gaaatccctc gccacctctg
     8401 atgtgtcagg catcctcaac gcacctgaga taaaatctat ttggctgttt ctgggttcga
     8461 actgatgatt atttggtgct aatacatgat ttaattccct gaatcctctt gcttttccca
     8521 ttggtggggt ctaagactcc tcgaagggaa taaaaatcaa tgatagtaga accatctgcc
     8581 aacttataaa tgtcggcatt atctccaacc gctaatacgc tgtaatactt gccaattttt
     8641 tgagacgtga tagtggctaa gagaggtgta cgctgccact cactgagaat gcattcttgt
     8701 atttcttgtg gtggagaatt tgttttaaac gagataataa cattttgttc ttctctgtag
     8761 ttataagggt tgacgcatcc t
//
LOCUS       DAZGAP010000190         8685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGAP010000190 DAZGAP010000000
VERSION     DAZGAP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.191"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..151
                     /gene="rrf"
                     /locus_tag="ACHLAM_004316"
     rRNA            36..151
                     /gene="rrf"
                     /locus_tag="ACHLAM_004316"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            206..282
                     /locus_tag="ACHLAM_004317"
     tRNA            206..282
                     /locus_tag="ACHLAM_004317"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Asp,seq:gtc)
     gene            444..1247
                     /gene="dkgB"
                     /locus_tag="ACHLAM_004318"
     CDS             444..1247
                     /gene="dkgB"
                     /locus_tag="ACHLAM_004318"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO7855763.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(1244..2158)
                     /gene="yafC"
                     /locus_tag="ACHLAM_004319"
     CDS             complement(1244..2158)
                     /gene="yafC"
                     /locus_tag="ACHLAM_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO7855764.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            2399..3199
                     /locus_tag="ACHLAM_004320"
     CDS             2399..3199
                     /locus_tag="ACHLAM_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7855765.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3276..4046
                     /locus_tag="ACHLAM_004321"
     CDS             3276..4046
                     /locus_tag="ACHLAM_004321"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7855766.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARHLKNIATRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(4093..5451)
                     /gene="mltD"
                     /locus_tag="ACHLAM_004322"
     CDS             complement(4093..5451)
                     /gene="mltD"
                     /locus_tag="ACHLAM_004322"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO7855767.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5523..6278)
                     /gene="gloB"
                     /locus_tag="ACHLAM_004323"
     CDS             complement(5523..6278)
                     /gene="gloB"
                     /locus_tag="ACHLAM_004323"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO7855768.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            6312..7034
                     /locus_tag="ACHLAM_004324"
     CDS             6312..7034
                     /locus_tag="ACHLAM_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7855769.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(7031..7498)
                     /gene="rnhA"
                     /locus_tag="ACHLAM_004325"
     CDS             complement(7031..7498)
                     /gene="rnhA"
                     /locus_tag="ACHLAM_004325"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO7855770.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            7563..8294
                     /gene="dnaQ"
                     /locus_tag="ACHLAM_004326"
     CDS             7563..8294
                     /gene="dnaQ"
                     /locus_tag="ACHLAM_004326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO7855771.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQDETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            8427..8503
                     /locus_tag="ACHLAM_004327"
     tRNA            8427..8503
                     /locus_tag="ACHLAM_004327"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8461..8463,aa:Asp,seq:gtc)
BASE COUNT         2170 a         2116 c         2103 g         2296 t
ORIGIN      
        1 aaccggcgac aagcgggtta ataaaacaga atttgcctgg cggccttagc gcggtggtcc
       61 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
      121 ctccccatgc gagagtaggg aactgccagg catcaaattt agcagtaagc cggtcataaa
      181 accggtggtt gtaaaagaat tcggtggagc ggtagttcag tcggttagaa tacctgcctg
      241 tcacgcaggg ggtcgcgggt tcgagtcccg tccgttccgc cacttattaa gaagcctcga
      301 gttaacgctc gaggtttttt tcgtctgtat ttctattatt gccagaatcg caaaaatcct
      361 ctgcgtttta cgctcttttt ccgcaacagt ctgaagccca taatcacctc agttaacgaa
      421 aatagcatta aaagaggcat attatggcta tccctgcatt tggtttaggt actttccgtc
      481 tgaaagacga cgttgttatt tcatctgtga aaacggcgct tgaacttggt tatcgcgcaa
      541 ttgataccgc acaaatctat gataacgaag ccgcagtagg tcaggcgatt gcagaaagtg
      601 gcgtgccacg tcatgaactc tacatcacca ctaaaatctg gattgaaaat ctcagcaaag
      661 acaaattgat cccgagtctg aaagagagcc tgcaaaaatt gcgtaccgat tatgttgatc
      721 tgacgctaat ccactggccg tcaccaaacg atgaagtctc tgttgaagag tttatgcagg
      781 cgctgctgga agccaaaaaa caagggctga cgcgtgagat cggtatttcc aacttcacga
      841 tcccattgat ggaaaaggcg attgctgctg ttggcgctga aaacatcgct actaaccaga
      901 ttgaactctc tccttatctg caaaaccgta aagtggtcgc ctgggctaaa cagcacggca
      961 tccatattac ttcctatatg acgctggcgt atggtaaggc cctgaaagat gaggttattg
     1021 ctcgtatcgc agctaaacac aatgcgactc cggcacaagt gattctggcg tgggctatgg
     1081 gggaaggtta ctccgtaatt ccttcttcta ctaaacgtaa aaacctggaa agtaatctta
     1141 aggcacaaaa tttacagctt gatgccgaag ataaaaaagc gatcgccgca ctggattgca
     1201 acgaccgcct ggttagcccg gaaggtctgg ctcctgaatg ggattaagcc tctctgacag
     1261 ctcctccggg agctgttttt acatgctcgc taaggaaatc gataaaagcc cggatgcgcg
     1321 tacttaccgc acggtcgctg taatatacgg cactgaatgg catttccact ggcaatactt
     1381 tatctgccat taactccacc aattctccgc gagcgatttc tctgtcgatc atgtagtcgg
     1441 acaaacacgc aatcccgttg ccactcaggc aaagctgttt cagtgtttcc ccactattgg
     1501 atgacaaacc gtacttcacc tcatgtaatt gtccatcact acaggctatc ggccaggtat
     1561 tgagggaagc gggttcagtg aatcccaggc aaacatgttg ctttaaatcg tcgatcgttt
     1621 ctggcttccc gtagcgggaa atataatcgg gggaggcgat aatttttcga taactgttaa
     1681 ataacggcct ggcacgtaag ctggaatccg ttaacgtacc agcgcgtatc gcgacatcca
     1741 cttttctttc gatcaaatta ataatcgttt cggaggagac tagcgataaa gtgacttccg
     1801 gatagcgttc acgaaaaggc ttaatcaacg gcatcagaaa gtgcagcacc actggagttg
     1861 cggcatcgat ccgtaacagt ccacgcggtg tattacgcgt ctccataatt tctgattctg
     1921 ccgctgccat ctcctgcaaa attgactgta cgcgacgaaa ataacgctcg ccttcttccg
     1981 tcaggctaag ttgccgcgtg gtccgattaa gcaggctaac gccaagtttc atctccagct
     2041 ttttcaccgc ccggcttacc gctgagtttg cttgccctaa ttgttccgct gcccggctaa
     2101 agctgccgct ttcgacgact gaaacaaaaa tggcgagttc ttccgacgtg gctttcattt
     2161 ttgctcctgt tgcaaaatag aagagatatt ttgaatttaa ttgtcattaa accatcagga
     2221 tgcgagatat gtcatccgat ttaatgttct caataatgag caaatttctg accggtgtaa
     2281 gcacttgctt acatagcaat atacaattgc tcgttgaaag agtgagctaa aatccctata
     2341 acagtagaac cctcccgagt gcggaagggt tgacgtaata gaggtttcaa agtcaaaagt
     2401 gcgaaaaaac acctatgcca tgcgctatgt tgctggacaa cctgcggaaa ggatcttacc
     2461 gccggggtct tttgcgagca tcggccaggc attaccacct ggagaaccgt taagtaccga
     2521 agagcgtatt cggatcctgg tgtggaacat atacaaacag caacgcgctg aatggttgtc
     2581 ggtattaaag aactacggca aagatgcaca tctggtgtta ttgcaggaag cgcagacaac
     2641 gccagagtta gtgcagtttg cgaccgctaa ctatcttgcc gccgatcagg tacccgcttt
     2701 cgtgctgcca caacatcctt ccggtgtaat gaccctttcg gcggcgcatc cagtgtattg
     2761 ctgcccgtta cgtgaacgag aacccatttt gcgtctggcg aagtcggcac tggtgacggt
     2821 ctatccattg cctgacaccc gcctgttgat ggtggttaat atacacgccg tcaatttcag
     2881 tttgggcgtg gatgtctata gtaagcagtt acttcctatt ggcgatcaga tagctcacca
     2941 cagcggcccg gtcattatgg cgggagattt caatgcctgg agccgtagaa ggatgaacgc
     3001 gttatatcgc tttgcgcggg aaatgtcgct gcgccaggtg cgttttaccg atgatcagcg
     3061 ccgtcgggcg tttggccgcc cgctcgattt tgttttctac cgtggtctga acgtcagcga
     3121 agcttctgta ctggttacgc gcgcttccga tcacaatccg ctactcgttg aattcagtcc
     3181 cggcaagcct gataaataag gtatgtcagg tctgccacag ggcagaccaa cgtttggcgc
     3241 tgcccttttt aatcaaacaa taaaaggaca gcgcaatgac aacacaatcc caccatgacc
     3301 acgtagaaaa gcagtttagc tcgcaggcca gtgagtattt aaccagtacc gtacatgcat
     3361 ccgggcgaga tttacagcgc ctggcggtgc gtctggctga ttatcctgat gcaagtgtac
     3421 ttgatatggg ctgcggagca gggcatgcca gctttgtcgc tgcgcagaac gtgagcgcgg
     3481 tggtggcgta tgacttatct gcccaaatgc tggatgtcgt ggcacaagct gccgaagccc
     3541 ggcacctgaa aaatatcgcc acccgccagg gatatgccga aagtctgcca tttgccgata
     3601 acgcatttga tattgttatc agccgttatt ctgcccatca ctggcatgat gttggtgcag
     3661 cactgcgaga agtgaatagg atattgaaac ctggcggtag gctgattgtg atggacgtaa
     3721 tgtctccggg tcacccagtg cgcgacgtct ggttacagac ggtagaagca ttacgcgata
     3781 cctctcacgt acgaaactac gccagcggtg agtggttgac gttaatcaat gaagccaatc
     3841 tgatagttga taatttaatt acagataagt taccgctgga attttcttca tgggtcgcga
     3901 gaatgcgtac gccagaagcg ttagtagacg ctattcgcat ttaccaacag agcgcatcga
     3961 cagaggtgaa aacgtatttt gccttgcaga atgatggctc tttcaccagt gatatcatca
     4021 tggtagaagc acataaagcg gcataaataa aaaaggcacc gggggaatcg gtgcctttta
     4081 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttatc
     4141 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatt acatctttga tgttcacgcc
     4201 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     4261 gctgttgttt gccagtcgct gtgcgctact gcctgcacca atcgtcaaac tttggcctgg
     4321 cttcagctta gatccgcgca gtttgttcca ctgctgcaaa tctttggtgc ttacgccgag
     4381 acgtgaagcg atacttgaaa gcgtgtcgcc agagcgtacg gtgtaaacac ggctgttaag
     4441 cggcgtattg tcggcaacca gcgtcgactg tacagcagca atttcgcctg aagccagaga
     4501 ttcacgcagt tgatctgcat gcttctttgg caccatcacg tactgcggac cacttgcgcc
     4561 cagcgtggag cctttcacgc ctgcgttgaa tgtcttcagc ttgctgacgg aaatccccgc
     4621 catatctgca accttcgcca tttcaaccgg gctgctcagg tgcacacgtg ccagagcacg
     4681 gctttcatcg gtcgttggca gacgtacgcc ataacgcttg ctgtttttga gaatatcact
     4741 caatgccagc atcttaggca cgtactgctt cgtttcctgc ggcaacggta acgaccagaa
     4801 gtccgtggat ttcccacgcg ctttgttcgt tttaattgcc ttcatgactc gaccttcgcc
     4861 gctgttataa gcagctacgg tcagaagcca gtcgccgtca aacattttgt tcagacgctg
     4921 catcatgttc agcgcggcag ttgttgaagc aacaacatcg cgacgcgcgt cataattgcg
     4981 ggtctgtttc aaaccataat tgcgccccgt gctcggaatg atctgccaga tgcctgcggc
     5041 attggcgcca gacgttgcgt gaggatcaaa agcgctctcc actatgggta gtagtaccag
     5101 ttccataggc atgttacgtt ttttaacttg cccggctatc cagtacatat acggctctgc
     5161 ccgtaaagtt acatcgtgga gatagctctt attgcgtaaa tatttctgtt tctgttcgcg
     5221 aatccggtca ttttccggaa ttcccatctt tagctcgtcg ccaatgaaag cccacaagtc
     5281 accatctggc gcgatagacg tcccatcgtc catccatcgt gcctgacttg taaactttgc
     5341 tgcttcccct tgaccagctg cagaaaggct ctgtgcgtgc tgttgaacgt tgccggtact
     5401 ctggcaaccc acgagcagga cagaggcgag taatatcgct tttgccttca tgtgtgtgtc
     5461 aatagttgct taaaagacga ccgatcataa cggcgaacgg agccgatgac aagaaagttt
     5521 tatcagaacc tatctttctt tgaccttaac catgcaaaac gttcttcagg ttgttgcaat
     5581 aatgtttctt cattaattac attaattaaa tcaatatctt ctgttcttaa aaaaacatta
     5641 atttgccgct catttttcag aattacgggt agtgttattt gattttttgc ccgtaactcc
     5701 ttaactttac gataataatc atttatggac aaatcgtgcg gaagaatact caaagcaaac
     5761 ttcatatttg ataaggtata ttcatgagca caacatacca atgtatcgtc aggtaacgca
     5821 cttaactttt taagtgattg atacatttgt gatgctgtcc cttcaaacaa ccgaccacac
     5881 ccaccagaaa acagcgtgtc gccgcaaaat agataaggtt tactgaagta acagatatgt
     5941 cctaaagtgt gacccggcgt ggcaattaca ctaaattcat gccccaaaac gaaggcagtt
     6001 tcgccatctt tgactacctg tgttgttccc ttatcttgtg tctcttgtgg accatacacc
     6061 acaatttgcg gaaacttttt caccagttct tttacgccgc caacgtgatc gtggtgatgg
     6121 tgggtgagaa atatggcctc cggttgccag ttattggcgg caatggcgtt taatactggc
     6181 tctgcgtctc cgggatcgac aatcaggcag cgacctgctt catcattcaa aacccagatg
     6241 taattgtcat caaaggcggg aatactgtta agattcatag attacctctc agtgtgaaac
     6301 ggaaggttgt gatgaaaccg gcaagagtcc ctcaaactgt cgtggctcct gattgctggg
     6361 gcgatttgcc ctggggagag ctttatcgca aggcgctgga gcgccagctc aacccgtggt
     6421 tcactaaaat gtatggtttt catctgctta agattggcaa tttaagcgca gaaatcaatt
     6481 gcgaagcgtg cgcggtttct catcaagtga atgtttctgc gcaaggactg cccgtccagg
     6541 tgcaggcgga cccacttcat cttccttttg ccgataaatc cgttgatgtt tgtctactgg
     6601 cacatacatt gccgtggtgc accgatccgc atcgtttatt gcgtgaagcc gatcgggtat
     6661 tgattgatga tggctggctg gtgattagtg gcttcaatcc catcagttta atgggattac
     6721 gcaaacttgt gccggtattg cgcaaaacct cgccctataa cagccggatg tttactctga
     6781 tgcggcagct ggactggctc tctttgttga attttgaagt gctacacgcc agccgtttcc
     6841 acgttctccc gtggaacaaa cacggaggaa aactattgaa tgcgcatatt cctgcgcttg
     6901 gttgcttaca acttattgtt gcccggaaac ggaccattcc tttaacgcta aatccgatga
     6961 aacagagtaa aaacaagcca cgaattcgcc aggcggttgg agccacccgg caatgtcgta
     7021 aaccacaggc ttaaacttcg acttgatagc ccgtatcttc cagtgtggga ttcatcgctg
     7081 cggcacgagc cagttcatcg caacgttcgt tttccgggtg tccggcatgg cctttaaccc
     7141 attcccattt gatttgatgc tgccccagcg cagcatcaag acgttgccag agatcgacat
     7201 tttttactgg ttttttgtct gctgttttcc agccacgttt tttccagtta tggatccact
     7261 gggtgatacc ctggcggaca tactggctgt cggtactcaa aatgacttcg caatgttctt
     7321 ttaacgcctc cagcgcgaca atagcggcca tcaactccat acggttgttg gtggtgcggg
     7381 tgtagcctgc gctaaaggtt ttctcgcgtc cgcgatagcg taaaatagcg ccgtaacccc
     7441 caggtccagg attacccaga cacgaaccat cggtgaaaat ttctacctgt ttaagcatct
     7501 ctggtagact tcctgtaatt gaatcgaact gtaaaacgac aagtctgaca taaatgaccg
     7561 ctatgagcac tgcaattaca cgccagatcg ttctcgatac cgaaactacc ggtatgaacc
     7621 agattggtgc gcactatgaa ggccacaaga tcattgagat tggtgccgtt gaagtggtga
     7681 accgtcgcct gacgggcaat aacttccatg tttatctcaa acccgatcgg ctggtggatc
     7741 cggaagcctt tggcgtacat ggtattgccg atgaattttt gctcgataag cccacgtttg
     7801 cagaggtagc cgatgagttc atggactata tccgcggcgc ggagctggtg atccataacg
     7861 cagcgttcga tatcggcttt atggactacg agttttcgtt gcttaagcgc gatattccga
     7921 agaccaatac cttctgtaag gtcaccgata gccttgcggt ggcgaggaaa atgtttcccg
     7981 gtaagcgcaa cagcctcgat gcgttatgtg cccgctacga aatagataac agtaaacgaa
     8041 cgctgcacgg ggcattactc gatgcccaga tccttgcgga agtttatctg gcgatgactg
     8101 gtgggcaaac gtcgatggct tttgcgatgg aaggagagac acaacagcaa caagatgaaa
     8161 caacaattca gcgcattgtg cgtcaggcaa gtaagttacg cgttgttttt gcgacagatg
     8221 aagagcttgc agcacatgaa gcccgtctcg atctggtgca gaagaagggg ggaagctgcc
     8281 tctggcgggc ataaatacct gtaaaaggcg ctaaaaatag cgacttgggc gatttttgca
     8341 gcaaacgatt caaaagatga gaaaaaccgt tgacgaaggg tgaggcaatc cgtaatattc
     8401 gcctcgttcc caacggaaca aaacgcggag cggtagttca gtcggttaga atacctgcct
     8461 gtcacgcagg gggtcgcggg ttcgagtccc gtccgttccg ccaaattcac tcatgaaaat
     8521 gagttcagag aaccgcaaaa atttttattt ttgcggtttt tttgtatttg aatcccgcca
     8581 tttctctgtt caatgatttt actctggcgt aagtgcgtaa ctctcgcttg ttgtctcatt
     8641 tattaaaatt caataatgat atcgaaccat tcaacttaaa tatat
//
LOCUS       DAZGAP010000191         8669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGAP010000191 DAZGAP010000000
VERSION     DAZGAP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.192"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            281..1171
                     /gene="alsR"
                     /locus_tag="ACHLAM_004328"
     CDS             281..1171
                     /gene="alsR"
                     /locus_tag="ACHLAM_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO7855772.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1230..2165
                     /gene="alsB"
                     /locus_tag="ACHLAM_004329"
     CDS             1230..2165
                     /gene="alsB"
                     /locus_tag="ACHLAM_004329"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO7855773.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2293..3825
                     /gene="alsA"
                     /locus_tag="ACHLAM_004330"
     CDS             2293..3825
                     /gene="alsA"
                     /locus_tag="ACHLAM_004330"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO7855774.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     HE"
     gene            3804..4784
                     /gene="alsC"
                     /locus_tag="ACHLAM_004331"
     CDS             3804..4784
                     /gene="alsC"
                     /locus_tag="ACHLAM_004331"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO7855775.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4795..5490
                     /gene="alsE"
                     /locus_tag="ACHLAM_004332"
     CDS             4795..5490
                     /gene="alsE"
                     /locus_tag="ACHLAM_004332"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO7855776.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDNHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5474..6403
                     /gene="alsK"
                     /locus_tag="ACHLAM_004333"
     CDS             5474..6403
                     /gene="alsK"
                     /locus_tag="ACHLAM_004333"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO7855777.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            6676..8661
                     /gene="yjcS"
                     /locus_tag="ACHLAM_004334"
     CDS             6676..8661
                     /gene="yjcS"
                     /locus_tag="ACHLAM_004334"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO7855778.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMV
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         2276 a         2091 c         2184 g         2118 t
ORIGIN      
        1 tgaaatccgc cttcccgatc tgttttacag agttacatct ggcagcgcaa tagcgttaaa
       61 gacactcact atccagcaac atctatcatc taataaacca ggaaaacaaa taacatcatg
      121 tttttaaagt gattaaatga aatgaaattt taagccactc tccattattc acaacaaaac
      181 aaactttata aattttattt ttttgtgaag ttgccagcat cttttctgtt cttgctgtgg
      241 tgatatagtg gcgtcttcaa ttcaaggaca agagaacgtg atgagccagt cagagtttga
      301 ttcagcgctt ccgaacggta tcgggttagc gccttacctg cgaatgaagc aggaaggaat
      361 gacagaaaac gaaagccgca tcgtggagtg gttactcaaa cccggtaacc tgagttgtgc
      421 acccgcaatt aaagatgtcg cagaagctct ggcggtatct gaagcgatga tcgttaaggt
      481 atcaaagctg ctggggttta gcggctttcg taacttacgc agtgcgctgg aagattattt
      541 ttctcagtca gaacaagtat tgccttccga gttggctttt gatgaagcgc cgcaggatgt
      601 ggtgaataag gtatttaaca tcactttacg caccattatg gaaggtcagt cgatcgtcaa
      661 cgttgatgag atccaccgtg ccgcccgctt tttctatcag gccagacagc gggatttgta
      721 cggtgccgga ggatcaaatg caatctgtgc tgatgtacag cacaagtttt tgcgcattgg
      781 cgtacgctgc caggcctatc cggacgctca catcatgatg atgtctgctt cgttgttaca
      841 ggaaggagat gtggtgctgg tggtgaccca ttccgggcga accagtgatg taaaagcggc
      901 tgtagaactg gcaaaaaaga acggggcaaa gattatttgt ataacgcata gctaccattc
      961 accgatagcg aagctggccg attatattat ttgctcaccg gccccggaaa caccgttatt
     1021 aggtcgtaat gcctcggcaa gaatattaca actaactttg ctggacgcgt tttttgtctc
     1081 tgtcgcccag ctcaacattg aacaagctaa tattaatatg caaaaaaccg gcgcaattgt
     1141 tgatttcttc tcaccaggcg cgctgaaata agaaaaaggt aattccgcca tcgcggagtt
     1201 acctatcccc ttaccgtgag aacatgatta tgaataaata tctgaaatat ttcagcggca
     1261 cactcgtggg cttaatgttg tcaaccagcg cttttgctgc cgccgaatat gccgtcgtat
     1321 tgaaaacact ctccaatcca ttttgggtag atatgaaaaa aggcattgaa gatgaagcaa
     1381 aaacgctggg cgtcagcgtt gatatttttg cctctccttc agaaggcgat tttcaatctc
     1441 aattgcagtt atttgaagat ctcagtaata aaaattacaa aggtatcgcc ttcgcaccat
     1501 tatcctcagt gaacctggtc atgcctgtcg cccgcgcatg gaaaaaaggc atttatctgg
     1561 tcaatctcga tgaaaaaatc gacatggata atctgaaaaa agcaggcggt aatgtggaag
     1621 gttttgtcac caccgataat gttgccgtcg gggcgaaagg cgcgtcattc attattgaca
     1681 agctgggtgc cgaagggggt gaagtcgcaa tcattgaggg taaagccggt aacgcctccg
     1741 gtgaagcgcg tcgtaatggt gccaccgaag ccttcaaaaa agcaagccag atcaagcttg
     1801 tcgccagcca gcctgccgac tgggaccgta ttaaagcact ggatgtcgcc actaacgtgt
     1861 tgcaacgtaa tccgaatatt aaagcgatct attgcgcgaa tgacacgatg gcaatgggtg
     1921 ttgctcaggc tgtcgcaaac gccggaaaaa cgggaaaagt gctggtcgtc ggtacagatg
     1981 gcattccgga agcccgcaaa atggtggaag ccggacaaat gaccgcgacg gttgcccaga
     2041 acccggcgga tatcggcgca acgggtctga agctgatggt tgacgctgaa aaatccggca
     2101 aggttatccc gctggataaa gcaccggaat ttaaactggt cgattcaatc ctggtcactc
     2161 aataaaacta aatgatgccg gatggtgatg ctaatgaagt gtcttatccg gccaacaaaa
     2221 taacctgaac gtagacctga taagtgtagc ccatcaggca ataatacgat atttcgtgca
     2281 gagaggttat ttatggccac gccatatata tcgatggcgg ggatcggcaa gtcctttggt
     2341 ccggttcacg cattaaagtc ggttaattta acggtttatc ctggtgaaat acatgcatta
     2401 ctaggagaaa atggcgcggg taaatccacg ctaatgaaag ttttatccgg aatacatgag
     2461 ccgaccaaag gcaccattac cattaataac attaactata acaaactgga tcataaatta
     2521 gcggcacaac tcggcatcgg gattatttat caagaactca gcgttattga tgaattaacc
     2581 gtactggaaa atttatatat tggtcgtcat ctgacgaaaa aaatctgtgg cgtcaatatt
     2641 atcgactggc gagaaatgcg tgtccgcgcc gccatgatgt tattacgcgt gggcttgaaa
     2701 gttgatttag atgagaaagt ggcgaattta tctatcagcc acaagcagat gctggaaatt
     2761 gccaaaacgc tgatgctcga cgccaaagtc atcatcatgg atgaacccac ctcctcactc
     2821 actaataaag aggtggacta tttgtttctg atcatgaatc agttgcgtaa ggagggtacg
     2881 gccatcgtct atatctcgca taaattggcg gaaattcgcc gtatttgcga ccgctatacg
     2941 gtgatgaaag acggcagcag cgtttgcagc ggcatggtga gcgatgtgtc aaatgacgat
     3001 atcgtccgtc tgatggtagg tcgcgaactg caaaaccgtt ttaacgcgat gaaggagaat
     3061 gtcagcaacc ttgcgcacga tacggttttt gaggtgcgca acgtcaccag ccgtgacaga
     3121 aaaaaggtcc gggatatctc atttagcgtc tgccggggag aaatattagg ctttgccgga
     3181 ctggtcggtt ccggacgtac tgaactgatg aactgcctgt ttggcgttga taaacgcgct
     3241 ggcggagaaa tccgtcttaa tggcaaagat atctctccac gttctcccct ggatgccgtg
     3301 aaaaaaggga tggcttacat cactgaaagc cgccgggata acggcttttt ccccaacttt
     3361 tctatcgctc agaacatagc tatcagccgc agtctgaaag acggcggcta taaaggcgcg
     3421 atggggttgt ttcatgaagt tgacgagcaa cgtaccgctg aagcccagcg cgaactgctg
     3481 gcgctgaaat gtcattcggt aaaccagaac atcactgaac tctccggcgg taaccagcag
     3541 aaagtcctga tctccaaatg gctgtgctgt cacccggaag tgatcatttt cgatgaacct
     3601 acccgtggca tcgacgttgg cgcgaaagcc gaaatttaca aagtgatgcg ccaactggcg
     3661 gacgacggaa aagtcatcct gatggtgtca tctgaactac ctgaaattat caccgtctgc
     3721 gaccgcatcg ccgtgttctg cgaaggacga ctgacgcaaa tcctgaccaa tcgcgatgac
     3781 atgagcgaag aggagattat ggcatgggct ttaccacacg agtaaaaagc gaagcgagcg
     3841 agaagaaacc gttcaacttt gcgctgttct gggataaata cggtactttt tttatcctgg
     3901 cgatcatcgt cgccattttt ggttcgctgt cgccagaata ttttctgacc accaataata
     3961 ttacccagat ttttgtgcaa agctccgtga cggtactgat cggcatgggg gaattcttcg
     4021 ctatcctggt cgccggtatc gacctttcgg ttggcgcgat tctggcgctt tccggtatgg
     4081 tgaccgctaa actgatgttg gcaggtattg atccgtttct cgcggcgcta attggcggtg
     4141 tactggttgg cggcgcactg ggggcgatca acggctgcct ggtcaactgg acgggactac
     4201 acccgttcat tatcaccctt ggcaccaacg ctattttccg tgggatcacg ctggtgatct
     4261 ccgatgccaa ctcggtatac ggcttctcat ttgacttcgt gaacttcttt gccgccagcg
     4321 taattgggat acctgtcccc gttatcttct cgctaattgt cgcgctcatc ctttggtttc
     4381 tgacaacgcg tatgcggctc gggcgcaaca tctacgcact gggcggcaac aaaaactcgg
     4441 cgttctattc cgggattgac gtgaaattcc acatcctggt ggtgtttatc atctccggtg
     4501 tttgtgcagg tctggcaggc gtcgtctcaa ctgcacgact cggtgccgca gaaccgcttg
     4561 ccggtatggg ttttgaaacc tatgccattg ccagcgccat cattggcggc accagtttct
     4621 tcggcggcaa ggggcgcatt ttctctgtgg tgattggcgg gttgatcatc ggcaccatca
     4681 acaacggtct gaatattttg caggtacaaa cctattacca actggtggtg atgggcggat
     4741 taattatcgc ggctgtcgcc cttgaccgtc ttatcagtaa gtaaggaatt gaacatgaaa
     4801 atctccccct cgttaatgtg tatggatctg ctgaaattta aagaacagat cgaatttatc
     4861 gacaaccatg ccgattactt tcatatcgat atcatggacg gccactttgt gccgaatctg
     4921 acgctctcac cgttcttcgt aagccaggta aaaaaactgg cgagtaaacc gctcgactgc
     4981 catctgatgg taacgcgtcc gcaggattac attgctcaac tggcgcgtgc gggagctgat
     5041 ttcatcactc tgcatccgga aaccatcaac ggccaagcgt tccgcctgat tgatgaaatc
     5101 cgccgtcatg gcatgaaagt ggggctgatt cttaatccgg aaacgccggt tgaggccatg
     5161 aaatactata tccataaggc tgataaaatt acggtcatga ctgtcgatcc cggttttgcc
     5221 ggacaaccgt tcattcctga aatgctggat aaacttgccg aactgaaggc atggcgtgaa
     5281 cgagaaggtc tggagtacga aattgaggtg gacggttcct gcaaccaggc aacttacgaa
     5341 aaactaatgg cggcaggggc ggatgtcttt atcgtcggca cttccggcct atttaatcat
     5401 gcggaaaata tcgacgaagc atggagaatt atgaccgcgc agattctggc tgcaaaaagc
     5461 gaggtacagc ctcatgcaaa aacagcataa cgtcgtagcg ggcgtggata tgggggcaac
     5521 gcatatccgc ttttgtctgc ggacagcaga aggtgaaacg ctacactgcg aaaaaaagcg
     5581 gaccgcagaa gttattgctc ccgacctggt gtcgggtatc ggcgaaatga ttgacgagca
     5641 actcaggcgc tttaacgctc gctgtcatgg tctggtgatg ggatttccgg cgctggtcag
     5701 taaagataaa cgcaccatta tttctacgcc taacctgccg ttaacagcgg cagatttata
     5761 tgatctcgcc gataagctcg aaaatacgct gaattgtccg gttgagtttt cccgcgacgt
     5821 taacctgcaa ctctcctggg acgtagtaga aaatcgcctt acgcaacaac tggttctggc
     5881 agcctatctc ggtacgggga tggggttcgc agtgtggatg aacggtgcgc cgtggacggg
     5941 tgcgcacggt gtggcaggcg aactgggcca tatccccctg ggagatatga cccaacactg
     6001 cgcgtgtggc aattctgggt gcctggaaac caattgctct ggaatggcgc taagacgctg
     6061 gtacgaacaa cagccccgaa attacccatt gagcgatctt ttcgtccatg cggaaaacgc
     6121 ccctttcgtc cagagtctgc ttgaaaacgc ggcacgagcc attgccacca gcattaatct
     6181 gttcgatccc gatgcggtga ttctgggtgg tggcgtgatg gatatgcccg ccttcccacg
     6241 cgagactctc attgccatga cccaaaagta cctgcgccgt ccactgccgt atcaggttgt
     6301 gcgctttatt gccgcctcat cttctgactt taatggcgct cagggtgcag caatattggc
     6361 gcatcaacgt tttttgccac agtcctgtgc taaagtccca tgagtaaagc gttggcacag
     6421 tagcgcaatg tcccgctgtg ccaacgacag gtacaatata tataataatt acataaaaca
     6481 gcccatatta ttcccagcaa tgatagttct actgtacttt tccctcttca gcacaaatgc
     6541 ctaataaaat ggcgcgttca gctcacactt ctacttatta aagtcgcaat ttcccaatta
     6601 tgataacgcc agctttaaat gacgtatttt gcatggatta catatttacg acataaatga
     6661 taaatggaga tttatatgaa taactctcgg ttattccgtt tgagcaggat tgttattgcg
     6721 ttaactgccg ccagcagcat gatggtaaat accgctaatg caacagatga agcgaaagcc
     6781 gccactcaat atacccaaca ggttaatcag aattacgcca aatcattacc gtttagcgat
     6841 cgtcaggatt ttgacgatgc tcagcgtgga tttatcgctc cgctgctgga tgaaggtatt
     6901 ctgcgtgatg cgaacggtaa aatttactac cgcgcgaatg attacaaatt tgatattaat
     6961 gccgctgcgc cggaaaccat aaaccccagc ctgtggcgtc agtcgcagat caacggcatt
     7021 tcaggcctgt tcaaagtcac cgataaaatg tatcaggtgc gcggccagga tatctctaac
     7081 attacgttcg ttgagggcga gaaaggcatt attgttatcg acccgctggt gacgccgcct
     7141 gcagcaaaag ccgcacttga tctttacttc cagcatcgtc cgcaaaaacc gattgttgcc
     7201 gttatctaca ctcacagcca cgccgaccac tatggcggcg tgaaaggtat tatctctgaa
     7261 gccgatgtta aatccggcaa agtgcaggtg attgctccgg caggctttat ggacgaagcc
     7321 atcagcgaaa acgtgctggc gggtaacatc atgagccgcc gtgcgctcta ctcttacggt
     7381 ctgttactgc cgcacaacgc gcaaggcaac gtgggcaacg gcctgggcgt gacgctggca
     7441 acgggcgacc cgagcattat tgcaccgact aaaactatcg tcagaactgg cgagaagatg
     7501 attatcgacg gcctggaatt tgacttcctg atgactccgg gtagcgaagc gccagccgaa
     7561 atgcacttct atattccggc cctgaaagcc ctgtgtaccg ccgagaacgc cacgcatacc
     7621 ctgcacaact tctacactct gcgcggcgcg aaaacccgcg ataccagcaa gtggaccgag
     7681 tacctgaacg aaacgctgga tatgtggggt aacgacgcgg aagtactgtt tatgccgcac
     7741 acctggccgg tctggggcaa taagcatatc aatgattata ttggtaaata tcgcgatacc
     7801 atcaagtaca ttcacgacca gaccctgcac ctggcgaacc agggctacac catgaatgaa
     7861 atcggcgaca tgattaagct gccgcctgcg cttgccaata actgggccag ccgcggctat
     7921 tacggttctg tcagccacaa cgcccgcgcg gtgtataact tctatcttgg ctattacgac
     7981 ggtaacccgg ctaacctgca tccgtatggt caggtggaga tgggtaaacg ttacgtgcaa
     8041 gctctgggcg gttctgcccg cgtcatcaac ctggcgaaag aagcgaacaa gcaaggtgat
     8101 taccgctggt cggcagaact gctgaaacag gtgattgccg ccaaccctgg tgaccaggtc
     8161 gcgaagaatc tgcaagcgga taactttgaa cagctgggct atcaggccga gtccgccaca
     8221 tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg aaggggtaca taagttcagc
     8281 cacggcacca ccggttcccc ggacaccatt cgcggaatgt cggtcgaaat gctgttcgac
     8341 tttatgtccg ttcgcctcga tagtgcgaaa gccgcgggta aaaatatcag cctgaacttc
     8401 aatatgagca acggcgataa cctcaacctg acgctgaacg atagcgtgct taactaccgt
     8461 aaaacgctgc aacctgaagc caacgcctct ttctacatca gccgtgaaga tctgcatgcc
     8521 gtgctgaccg gacaagtcaa aatggtggat ctggtaaaag cgaagaaagc caaaattatt
     8581 ggcaatggcg cgaaactgga agaaattatt gcctgtctgg ataatttcga tttgtgggtg
     8641 aatatcgtaa ccccaaatta atgctactc
//
LOCUS       DAZGAP010000192         8588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGAP010000192 DAZGAP010000000
VERSION     DAZGAP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.193"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..407)
                     /locus_tag="ACHLAM_004335"
     CDS             complement(<1..407)
                     /locus_tag="ACHLAM_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7855779.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKVDESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQP"
     gene            complement(409..657)
                     /locus_tag="ACHLAM_004336"
     CDS             complement(409..657)
                     /locus_tag="ACHLAM_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7855780.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            825..1409
                     /locus_tag="ACHLAM_004337"
     CDS             825..1409
                     /locus_tag="ACHLAM_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855781.1"
                     /translation="MNMKNSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINVQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEREHVSMEWLVNGYGDEPSMKKEPIEQTKRTKKQ
                     RIDDGDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTVLLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKAGA"
     gene            1681..2073
                     /locus_tag="ACHLAM_004338"
                     /pseudo
     CDS             1681..2073
                     /locus_tag="ACHLAM_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(2046..3074)
                     /gene="torT"
                     /locus_tag="ACHLAM_004339"
     CDS             complement(2046..3074)
                     /gene="torT"
                     /locus_tag="ACHLAM_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO7855782.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMAEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQEQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            3157..5901
                     /gene="torS"
                     /locus_tag="ACHLAM_004340"
     CDS             3157..5901
                     /gene="torS"
                     /locus_tag="ACHLAM_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO7855783.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPQRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDDIDIARANQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5973..7046
                     /gene="yccM"
                     /locus_tag="ACHLAM_004341"
     CDS             5973..7046
                     /gene="yccM"
                     /locus_tag="ACHLAM_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7855784.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLNFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(7094..7267)
                     /gene="gnsA"
                     /locus_tag="ACHLAM_004342"
     CDS             complement(7094..7267)
                     /gene="gnsA"
                     /locus_tag="ACHLAM_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO7855785.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(7257..7487)
                     /gene="ymcE"
                     /locus_tag="ACHLAM_004343"
     CDS             complement(7257..7487)
                     /gene="ymcE"
                     /locus_tag="ACHLAM_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO7855786.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            complement(7462..7650)
                     /gene="ymcF"
                     /locus_tag="ACHLAM_004344"
     CDS             complement(7462..7650)
                     /gene="ymcF"
                     /locus_tag="ACHLAM_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO7855787.1"
                     /translation="MTPHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(7661..7873)
                     /gene="cspG"
                     /locus_tag="ACHLAM_004345"
     CDS             complement(7661..7873)
                     /gene="cspG"
                     /locus_tag="ACHLAM_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO7855788.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            8159..8371
                     /gene="cspH"
                     /locus_tag="ACHLAM_004346"
     CDS             8159..8371
                     /gene="cspH"
                     /locus_tag="ACHLAM_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO7855789.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         2254 a         2087 c         2089 g         2158 t
ORIGIN      
        1 ggttgttgcg cgactttcag aacttcgctg acgatcgcga ttcgtgcatc agccacctgg
       61 cgctggtttt cagtcagggc attaaggcgt tcgaccatca gttgtggtga tccgcgatat
      121 gcctccaccg catcaaccac ggcaggcgag cgtctggcct ttgtcaccac tggagccggt
      181 gattcatcga ctttttgagt catcagttgc agggcgtagc gttcacgtaa tgcctgctgc
      241 gtcacttcag gtaaacagtc gatgctgtat tcagttgctt tagagcctga gcgacgacgg
      301 gaacaacagc ttttcccttg tacgtaacgt tgtaatgcct ggcgaatacc ttttgttgta
      361 gcaggcattc cgggcgcgcc aatcagttct tttgcaatga catacatatc atgacgcctt
      421 tctcatatag ctcttaacct ggtatcgact gggccagatt tcttcaggcg ataccccaag
      481 agcatcagca attatttgct gatacggtcg gcaaggagta cgtaatacgc ttttcagcga
      541 atccctgctg taaccagcct ttaaagataa agagcgcagt gataagccag ccatgtgtaa
      601 gcgcgccttg atgatttcag gcggccaatc gtgatctgaa acttctcttc tactcatact
      661 tcctctattc taaaaagtta cccgcgcgga tattcgtgcg gatatctgta cgggtaaata
      721 atagaacaaa caaaagaacc aaatcaaggt attttgttct ttttattctt tgcggtgttt
      781 ttgttattaa tatcaataag ataaatacaa aggaacctga gagcatgaac atgaaaaaca
      841 gttcttttct cgacgaagga aaagaaccag tgatcgaacg gatttttcgt cttgcagagc
      901 gttacaaatc gagaagtgag gcggctagag catggggaat aaacgttcag acattgcaga
      961 actactacaa aaggagaaac aatcagccgc caccacaacc aagaaagcac catctccaga
     1021 acattgcaga aagagaacat gtatctatgg agtggctagt caatggttat ggtgatgagc
     1081 caagcatgaa aaaagaacca attgaacaaa caaaaagaac aaaaaaacaa agaattgatg
     1141 atggtgattc catcatcatg agggcgtggg actcgctaac ttcatctgaa caagagattt
     1201 tgtccaactt attgatcaga aaaggggccg aactactaac cgttttgctt gatacagata
     1261 ttcaaaaact gcactcattg agcggagtca aaagagcact agcattgagt ctacaagagt
     1321 tacccgatga tactgtaaga gagatttatg aagaatgtga agctaaggtt aatcatttaa
     1381 atgtaaccca gaaaaaggct ggtgcgtaag ggagtggtgc aatttttgtg ctgtagtaca
     1441 ctcctttaaa gacaatctgg agaaaaacgt tttaagcctt tttaaagcca ttttaagcgt
     1501 caatccgtag tatcaaaagt tttgccgatt ttagaataaa cgattaatta ttgcacaatc
     1561 ggtatcaaat agactttaag cgctcatcca gacatcacct gttttatttc agtatcttac
     1621 aggtgatttc acctcctttc actaaaaccc tcgttggtat caaatgattc acctggttac
     1681 accaaaccgc tggaactgcg cgaactggta gtacgggtga aaaatctgct ctggcgaatc
     1741 gacctcgcgc gacaagctca accgcacaca caggacaact gctatcgctt tgccggttat
     1801 tgcctgaatg tgtcgcgcca tacgctggag cgggatggcg agccgattaa actgacccgc
     1861 gcagagtatg aaatgttggt ggcatttgtg acgaatccgg gcgaaattct tagccgtgaa
     1921 cgtctgctgc gtatgctctc tgcgcgtcgg gtggaaaacc ctgacttgcg caccgtcgat
     1981 gtgttaattc gtcgtttacg ccataaactc agcgcggatt tactggtgac gcaacatggc
     2041 gaaggttatt tcttagccgc tgatgtgtgc tgataaaaat agaccggacg aaatcccccc
     2101 ggtgacagcg agcggcggat atgttcgcgg tcggcatttt tcggcgtcag aactaaaatc
     2161 ggtgggctga cattatcaga caccgattgc tcctgtaatt gcctgatggc ctgctcaacc
     2221 gccagttccc cctgccagac catctgatcg ctggcagcca taatcactct tccccgcttc
     2281 agcccgcgat acacctgatg tgaaagataa aacgacacca cggtaagcgg cgttttcagg
     2341 ttacgccctt cacccattgc cgcctctgcc gcaatggccg ttccggcaac gacgtcgatt
     2401 tctggatggc gctccagcat ctcctgcaac aggttacgct ggatttcaat atcgttatcg
     2461 ccaagcgcaa tatcaacaat acgtaccggg cttccagcaa tagctgcgcg aaaaccctcg
     2521 gccatctctt tactgccccc ggcgttatcg ggtccgggca tcaacagcac gttcagtggg
     2581 ttaccgtgcg cccattgcac cagatatcgc ccaggttgat agcccatctg aaaccagggc
     2641 acaccaacgc ggcttttcac ctggggagca tcaatagcat ttaccagttc gatcaccggc
     2701 agatttgcta cctgcttttg cagatcggga aatgacgtcg tgctactacc gagcaaaatg
     2761 gcctctgcgc cccactgttt acactggtcg atttgtgctt gctgggtagc caactggctg
     2821 tagccgcctg cctccagcac ttttaaatcc acaccgtagc ggcgagcagc ctcctgcata
     2881 ccatagttca acgataacca gtatgaatct ttcaaactgg gataaagcgc gcacagtttc
     2941 catgcgcgtt tggcttttag cggcgtagag gcttgcaccg tgtaatgctg tgcatcatgc
     3001 cagcgcaaca ggttatcagc cgaaaatgcc gataacatga aaagggaaag tagtaacaat
     3061 agcagtacgc gcatgatagc ctcatcaata ataaggcttt atgctagatg cattccgctt
     3121 tgcgactcaa cctttttcac ccaaagtgca ccgaccgtga atttaaccct gacccgaaga
     3181 ctctggatgg gctttgccct gatggcgctg ttaaccctga ccagtaccct ggtgggatgg
     3241 tacaacctgc gctttatcag ccaggtggaa aaagacaaca ctcaggcatt gattcctacc
     3301 atgaatatgg cgcgccagtt gagcgaagcc agcgcctggg aacttttcgc cgcgcagaac
     3361 ctgaccagtg ccgataacga aaagatgtgg caggcgcagg ggcgaatgct caccgcacaa
     3421 agcctgaaga ttaatgcgtt gctgcaagcg ttacgggaac aagattttga caccaccgct
     3481 attgaacaac aggagcagga gatctcccgt tcgttacgtc agcaagggga actggtgggg
     3541 cagcgtttgc aacttcgcca gcaacaacag caactcagtc agcagatagt cgccgccgcc
     3601 gatgaaatcg cacgcctggc gcaaggtcag gcgaataatg cggcaacctc cgccggagcg
     3661 acccaggccg ggatttacga tttgatcgaa caacatcagc gtcaggccgc agaaagtgca
     3721 ctcgatcggc tgattgatat cgatcttgag tatgttaacc agatgaatga actgcgcctt
     3781 agcgccctgc gggtgcagca aatggtgatg aatctggggc tggaacagat ccagaaaaat
     3841 gcgccaacgc tggaaaagca gctcaataat gcggtgaaaa ttctgcaacg tcggcaaata
     3901 cgcattgaag atccgggtgt tcgtactcag gtagcaacaa cgttaactac cgttagccaa
     3961 tatagcgatt tgctggcgct gtatcagcag gacagtgaaa tcagcaatca cctgcaaact
     4021 ctcgcccaaa ataacatcgc ccaattcgcg cagtttagta gcgaagtcag tcagctggtc
     4081 gacaccattg agctgcgtaa tcagcacggg ctggcacatc tggaaaaagc cagtgcacgc
     4141 gggcaataca gcctgttatt gctgggaata gtttctcttt gcgcactgat tctgatcctc
     4201 tggcgcgtgg tttatcgctc agtcacgcgc cctcttgcag aacaaacgca ggcgctacaa
     4261 cgcttgctgg atggtgatat cgactcccct ttcccggaaa ccgctggcgt gcgggaactg
     4321 gacaccatcg ggcggctgat ggatgcgttt cgcagcagtg ttcatgcact gaatcgccac
     4381 cgtgaacagt tggcggcgca ggtcaaagcg cgtacagctg aattgcagga actggtgata
     4441 gaacaccgac aggcacgggc ggaagcagaa aaagccaacc aggcaaaatc agcgtttctg
     4501 gcggcgatga gccatgagat ccgcacaccg ctgtacggta ttctcggtac cgcacagcta
     4561 ctggcagata accccgcact taacgctcag cgtgatgatt tgcgggcaat taccgactct
     4621 ggcgaatcgt tgctgaccat cctcaacgat attctcgatt attcggctat cgaagcaggc
     4681 ggcaagaatg tttcggtcag cgatgaaccc tttgaaccgc gcccgttgct ggaaagtacc
     4741 ctgcaattaa tgagcggacg ggtgaaaggt cgcccgattc gcctggcaac ggcaattgcc
     4801 gacgatgtac cgactgcgtt aatgggcgat ccgcagcgta ttcgtcaggt cattaccaac
     4861 ctgttgagca acgccctgcg tttcaccgac gaagggcaca ttatcctgcg tagtcgcact
     4921 gatggcgaac aatggctggt cgaagtggaa gacagcggct gcggtattga tcccgcgaaa
     4981 ctggctgaaa tcttccagcc atttgttcag gttagcggca aacgcggcgg caccgggctg
     5041 gggctgacta tcagtagccg tctggcccag gcgatgggtg gcgaactgag cgccaccagc
     5101 acgccggagg ttggaagctg tttttgttta cgcttgctgt tacgtattgc cactgcaccc
     5161 gtgccaaaaa cagtcaatca ggcggtgcgt ctggacggtt tacgtttgct gttaattgaa
     5221 gataacccgc taacccagcg gattaccgtt gagatgttga acagcagtgg ggcgcaggtc
     5281 gttgccgtag gaaatgccgc gcaggcttta gagacactgc aaaatagtga accatttgct
     5341 gtcgcactgg tggattttga tctgccggat atcgacggca ttacgcttgc ccggcaactg
     5401 gcacagcaat atccgtcgct ggttttgatt ggctttagcg cccatgtcat cgacgaaacg
     5461 ttgcgccagc gtaccagttc gctatttcgc gggattatcc ctaaaccggt gccgcgtgaa
     5521 gtgctcggtc agttactggc gcactatctc caactgcaag tcaataacga tcaaccgctg
     5581 gatgtatcgc aactcaatga agatgctcag ttaatgggga cggagaagat ccacgaatgg
     5641 ctggtattat ttacacaaca tgccctgccg cttctcgatg acatcgacat tgcccgcgcc
     5701 aatcaggata gcgagaaaat aaagcgtgcc gcacatcagc taaaaagcag ttgctcgagc
     5761 ctgggaatgc gtatcgccag ccagctatgc gcacaactgg agcagcagcc attatctgcc
     5821 ccccttccac acgaagaaat tacacgcagt gttgccgctc tggaagcatg gttgcataag
     5881 aaagacctga acgcgatttg aatcaatatc agccagttaa gaacaactag aatagtggca
     5941 tttggtatgc aacttaacgg actggcaaaa tcatgacaga gaaaagaaga acccgctggc
     6001 agcggcggcc aggcacgacg ggcggcaaat taccgtggaa tgactggcgc aatgccacga
     6061 cctggcgtaa agcgacgcaa ttattactgt tggcaatgaa tatttatatt gccatcacgt
     6121 tctggtattg ggtgcgctat tacgaaacgg caggtagtac gacatttgtc accagaccgg
     6181 gaggtatcga aggctggcta ccgattgccg gtctgatgaa tctgaaatat agccttgcaa
     6241 caggccagtt accgtccgtc cacgccgccg cgatgctgtt attggtcgct tttatcgtca
     6301 tcagtctatt attaaaaaag gccttttgtt catggttatg cccggttggt acgctttctg
     6361 aattaatcgg cgatctcggt aacaaactgt ttggtcggca atttgtcctt ccccgctggc
     6421 tggatactcc tctgcgcggc gtgaagtatt tgctgttgag tttttttctc tatattgcgt
     6481 tattgatgcc cgctcaggcg attcactatt ttatgttgtc gccctacagc gtggtgatgg
     6541 acgttaaaat gctcaatttc tttcgtcata tggggaccgc gacattaatc agcatgatcg
     6601 ttttgctgat tgccagcctg tttattcgcc atgcctggtg tcgttatctt tgcccatatg
     6661 gcgcgctgat gggcgtggtt tcgctattat cgccgtttaa gattcgtcgc aatgccgaaa
     6721 gttgtatcga ctgtggcaaa tgcgcaaaaa attgcccatc acggatcccg gtcgataaat
     6781 taattcaggt acgaacagtg gaatgtaccg gctgtatgac ttgcgtagag tcatgtccgg
     6841 tagcctcaac attgaccttt tcactgcaaa aacctgcggc aaataaaaaa gcctttgcgt
     6901 tgtctggctg gttaatgacg ctactgattc tggggattat gtttgcggtg attggttacg
     6961 caatgtatgc gggagtatgg caaagcccgg tgccggagga attgtaccga cgcttaattc
     7021 cacaagcgcc aatgattggc cactaaatta gcaacaagcg gaacatcacc tcgcacgggt
     7081 gatgttcaca aaatcacatt attttgattt tgacatcata actttcaagc ccggtcattt
     7141 tttctcgtgc ggtgaagcga atttcagaga cttctttccc tgtattcttg ttcagctcgg
     7201 cgattttttg cgcgataaag tcagcgattt ccgtttcggc ttgttttttt aactcttcaa
     7261 tattcactgt taacctcttc tggtcttgaa agacagcaac acgccagtaa ggtgttggtt
     7321 cccggtaccc tggtcaggta agaaatgtta taagccatct ccccgaaaga ttcaataatc
     7381 aacaggttat cgcttactat acatacagcg ttgaagaaaa ataaggcaga tataaaaaag
     7441 gctccacgag ggagcctgat attattttgt gaaatccagc ggcgcatggt cagtgcgtat
     7501 ttgcagcgcg acgttatcaa aggtgatcat cgtcgattta caaaaaatac actttgcccc
     7561 taaaggattt cgctccgtca catcgaatgc cgatgtgcgg tactgtgaac catgacaaca
     7621 tggacagcgg aaatggagat gtggagtaat aacggcaacc ttagagcgta acaacgttcg
     7681 ctgccgcggg accacgttgc ccctgctcaa tagaaaattc aactttctga ttttcgttca
     7741 gcgtgcggaa ttcattgctc tggatggcgg tgaaatggac gaaaacgtct ttgctgccat
     7801 catcaggagt gataaagcca aaacctttat ctgcgttaaa ccattttact aaaccagtca
     7861 ttttattaga catattttac ttccttcatt ttgggccttt cggcaaacag ggctttagta
     7921 cagaaactac ttagtgttca ggagagagac tcaaaagaag gggataatgc ctgataatga
     7981 gaactgcttt agtaaactac tttgtatgct gtctgtcttt caaaccgacg cagctattaa
     8041 cgcatgataa ttgagattaa gcaaatttta ttttagccgt ccggcggtct taaatatcgc
     8101 tggctttatt ttattaaccc cgtatagtgc agcgcagtat tttcagtaag gaatttgttt
     8161 gtcccgtaaa atgacaggaa ttgtcaaaac ctttgatcgc aaaagcggca aaggattcat
     8221 tatcccctcc gacggtcgta aagaagtcca ggtccatatt tccgcattca ctccccgcga
     8281 cgcagaagtg cttataccag gattacgcgt ggaattttgc cgcgtaaatg gcctgcgagg
     8341 accaacagcg gcaaatgttt atctctctta atatgagttg cttgtttaaa gttggctggt
     8401 tttattggaa tagctcaata gtcaacacca taaattataa cgcaagttac tttttcaccg
     8461 agcaacaaat ccatttaagc gaatatctct gcacctaata gttataaatg ccacttaaca
     8521 gccagttata gtaccacttg aaactatatt accaacaata gttaatagct ttcttaacaa
     8581 ttttctta
//
LOCUS       DAZGAP010000193         8578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGAP010000193 DAZGAP010000000
VERSION     DAZGAP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.194"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..1527
                     /gene="dcuC"
                     /locus_tag="ACHLAM_004347"
     CDS             160..1527
                     /gene="dcuC"
                     /locus_tag="ACHLAM_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7855790.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAIGAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1571..2068)
                     /gene="sspB"
                     /locus_tag="ACHLAM_004348"
     CDS             complement(1571..2068)
                     /gene="sspB"
                     /locus_tag="ACHLAM_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO7855791.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2074..2712)
                     /gene="sspA"
                     /locus_tag="ACHLAM_004349"
     CDS             complement(2074..2712)
                     /gene="sspA"
                     /locus_tag="ACHLAM_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO7855792.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3107..3499)
                     /gene="rpsI"
                     /locus_tag="ACHLAM_004350"
     CDS             complement(3107..3499)
                     /gene="rpsI"
                     /locus_tag="ACHLAM_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO7855793.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3523..3951)
                     /gene="rplM"
                     /locus_tag="ACHLAM_004351"
     CDS             complement(3523..3951)
                     /gene="rplM"
                     /locus_tag="ACHLAM_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO7855794.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4170..5297)
                     /gene="zapE"
                     /locus_tag="ACHLAM_004352"
     CDS             complement(4170..5297)
                     /gene="zapE"
                     /locus_tag="ACHLAM_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO7855795.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5491..5889
                     /gene="zapG"
                     /locus_tag="ACHLAM_004353"
     CDS             5491..5889
                     /gene="zapG"
                     /locus_tag="ACHLAM_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO7855796.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6043..7410
                     /gene="degQ"
                     /locus_tag="ACHLAM_004354"
     CDS             6043..7410
                     /gene="degQ"
                     /locus_tag="ACHLAM_004354"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO7855797.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7500..8567
                     /gene="degS"
                     /locus_tag="ACHLAM_004355"
     CDS             7500..8567
                     /gene="degS"
                     /locus_tag="ACHLAM_004355"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO7855798.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         2069 a         2170 c         2202 g         2137 t
ORIGIN      
        1 aaataatcat aagcgcatat tttttaatga aaaatcacct catctacaat taaaaacacg
       61 acatccgcac cataaatagc cttgcaaaaa atataacatc attgttttca atctgccgtt
      121 tatgggattg accattttct tttgacacgg agttcaacaa tgttcggcat aattatatct
      181 gtcatcgtat taattacgat gggctatttg atcctgaaaa actacaaacc tcaggtggtg
      241 ctggctgccg caggtatctt cctgatgatg tgcggtgtct ggttagggtt cggtggtgtg
      301 ctcgatcccg ccaaaagcag cggctacttg atcgtcgata tttataatga aatcctgcgc
      361 atgctgtcca accgcattgc tggattgggg ctgtcgatta tggcagtggg cggttatgcc
      421 cgctatatgg agcgcatagg ggcaagtcgc gcgatggtga gcttgttaag ccgcccgtta
      481 aaactcattc gctcgccgta tattattctg tcggcaactt acgtcatcgg ccaaatcatg
      541 gcgcagttta ttaccagcgc ctccggtctg ggtatgttgc tgatggtcac cttatttccg
      601 acgctggtga gtctgggagt aagtcgtctc tctgcggtgg cggttatcgc aaccaccatg
      661 tccattgagt gggggattct ggaaacgaac tccatttttg ctgcccaggt cgcaggaatg
      721 aaaattgcca cttacttctt ccactaccag cttccggtcg cctcttgcat cattatctcg
      781 gtggcgatct cccacttttt cgtgcaacgc gctttcgaca aaaaagataa aaatatcaat
      841 cacgaacagg cagagcaaaa agctctcgat aatgtcccgc cgctctatta cgccatttta
      901 ccggtgatgc cgttaatcct gatgctcggc tcgctgttcc ttgcccacgt cgggctgatg
      961 cagtcagaac tgcatctggt ggtggtgatg ttactgagtt tgacggtgac gctgtttgtt
     1021 gagttcttcc gcaagcataa cttgcgcgaa acaatggacg atgtgcaggc gttttttgac
     1081 ggcatgggta cgcagtttgc caacgtggta acgctggtgg tcgcgggtga aatatttgcg
     1141 aaaggcttaa cgacgattgg cactgtcgat gcggttatca ggggggcgga gcattctggt
     1201 ctgggcggta ttggcgtgat gattattatg gcgctggtca ttgccattgg tgccattgtg
     1261 atgggctcag gcaatgcgcc gtttatgtca tttgccagtc ttattccgaa tatcgcagcc
     1321 ggactacatg taccagcggt tgtaatgatt atgccgatgc attttgccac gacgctagcg
     1381 cgcgctgttt cgccgattac tgcggtggtg gtcgttacgt caggaattgc aggcgtttcg
     1441 ccttttgcgg tagtgaagcg gacagcgatc cccatggcag tcggtttcgt ggtgaatatg
     1501 attgccacta tcacgctatt ttattaagcg attaaaaaga caaaacaggc cgccagggcc
     1561 tgttttcatt ttacttcaca acgcgtaatg ccggtcgacc accgcgtggt ggctgcggag
     1621 gttcatcgtc aggatgagtg tcatcatcgt gatctggctt gtcgccatca ataaccgaca
     1681 taacggtttc gttgtctgcc gatgcctctt catcattcat gatgctggca tcttcatcgt
     1741 aggcagcttc aggttcaaac atcgtgcctg cgccattttc acgggcgtag atagccagca
     1801 cggcagccag cggcacagaa acctgacgcg gaatgccacc aaagcgcgcg ttaaagcgca
     1861 cctcatcatt cgccagttcc agattgccga cagctcgcgg cgcaatgttg agtacgattt
     1921 gcccgtcacg cgcatattcc ataggaacct gcacgccagg gagcgtcaca tccaccacca
     1981 ggtgcggcgt gagctggtta tccagcaacc actcatagaa tgcacgcagc agatagggac
     2041 gacgtggtgt tagctgtgac aaatccatac agattaactc cggcccagac gcatttcacg
     2101 ttcagcttca gttaaagaag caaggaaaga gtcacgctca aagacgcggg tcatatagcc
     2161 tttcagctct ttcgcacccg ggccgctgaa ctcgatgccc agttgcggca gacgccacag
     2221 cagaggagca aggtagcaat cgaccaggct gaactcatcg ctcaggaagt acggcttctg
     2281 accgaagacc ggcgcaatcg ccagcagttc ttcgcgcagt tgcttacgtg cggcatcggc
     2341 ttcagaagct gaaccgttga tgatggtgtt catcagcgtg taccagtctt tttcgatgcg
     2401 atgcatgtac agacggcttt caccgcgagc taccgggtaa acaggcatca gtggcggatg
     2461 cgggaaacgc tcatccagat attccataat gatgcgagat tcccacaggg tcagctcacg
     2521 atccaccagg gtcggaacgc tctgattcgg gttgaggtca atcagatcct gaggcggatt
     2581 gtccttttcc acgtgttcga tctcgaaact tacacctttc tcagccagca caatgcggac
     2641 ctgatggcta tagatgtcag taggaccgga aaacagcgtc attaccgaac gtttgttggc
     2701 agcgacagcc atgaaaacct ccaggtatag tcagaatttt tactgctacc agccaccagg
     2761 tggccagtca gaagttgtgt tacccagtaa ggaacgactc tctttgttcg aaaatcaaac
     2821 aaaaaatgag caatacccga catttgggca gaaaattgga tgatagttta ccagattttg
     2881 cgacctttgt ggtgagtcga tgccggaaat ggggaaaaag agatgcgctt tagtctgaaa
     2941 tagttgactt agtcccttat tggcgatgtg gtttttgttt tacctgtctg tcaggtggca
     3001 gcaaaaagca acttcccatt ttttacgctg atgcagattt tagccataaa aaaacccgcc
     3061 gaagcgggtt ttttcgcaaa ttgttttctg ccggagcaga agccaattaa cgtttggaga
     3121 actgcggacg acgacgtgct ttacgcagac cgactttctt acgttcaacc tgacgagcgt
     3181 cacgagtaac gaagccagct ttacgcagtt cagaacgcag ggactcgtcg tattccatca
     3241 gagcgcgggt gataccgtga cggatcgcac cagcctgacc agagatacca ccacctttaa
     3301 cggtgatgta caggtccagt ttctcaacca tgtcgaccag ttccagcggc tgacgaacta
     3361 ccatgcgagc agtttcacga ccaaagtact gttccagaga acgttggttg attacgattt
     3421 taccgttgcc cggtttgatg aaaacgcgag ctgcggaact tttgcggcga ccagtgccgt
     3481 agtattgatt ttcagccatt gcctataatc ccgattagat gattagatgt caagaacttg
     3541 cggttgctgt gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat
     3601 agcacgaccc agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc
     3661 aggacggcga gcaatcatct cttcaaaggt cgcttgtttg ataccaccga tgtggccggt
     3721 gtggtgatag tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt
     3781 cagaacgatg atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc
     3841 gcgcaggcga cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac
     3901 aacataccag tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt
     3961 acccaataaa aagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca
     4021 cgacaaagtc cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg
     4081 ggaaaaaaac tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat
     4141 cgaccccttt ttgtgatttg tggcaggttt taacccgcca aatgctcgcg cttcagatac
     4201 tcttcgcttt gcatctcttg cagacgtgac aggcaacgct ggaactcaaa tttcagccgc
     4261 tcgccctgat aaatgtcata tagcggcact tctgcactca ccactaattt gacatggcgc
     4321 tcgtaaaact catccaccag cgcaataaaa cgtcgcgctt cgctctccat caaccgcgtc
     4381 ataactggta catcaaacaa catgactgtg tgaaagaggc gtgagagcgc aatatagtca
     4441 tgctgactgc gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg
     4501 acgcccattg tcgccagcgg ccgatggttg atttctaatg tcggtgaatt ttctcgtttc
     4561 gcccccgcca gcgccaacca tagtttatcc atttgcgccc gggtttcatc gttgaggggc
     4621 gaaagccaca gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg
     4681 ttcattacat cacaatgctg tttaatggca tcgattgcag gcaaaaaacg cgcacgttgt
     4741 aggccatttc gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg
     4801 cgggcgaaca gggctttcat cagaccgcca agtagcatgg catcggtaat atcagaaaca
     4861 aaaaattcgt caaaacagag cacgtcagtt tctgctttaa agcgatcggc aataatttcc
     4921 agcggatcgg tctgcccctg caaagcagtt agctcttcat gtacccgcag cataaaacgg
     4981 tgaaagtgca gacgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc
     5041 caggttttcc cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt
     5101 gtgtcttcgc gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg
     5161 gctggaggcg tgctattgat gagttcctga taaataattt ccaggcggct gacggcctct
     5221 ttttgaacgt cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt
     5281 ggggtaacgc tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca
     5341 cggttgacga aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg
     5401 tggaattaac ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc
     5461 tacggaaaca aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta
     5521 gtcgtcggca tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag
     5581 caacaggcgt tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa
     5641 gagctggtta gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat
     5701 cgccagctgt atcaacacat ggcgaaaagc tccagcagcc tgctgccgga actgtctgct
     5761 gaagcaaacc cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg
     5821 gtgcaaatgc ctcgcgacta ttctgaaggc gcatccggcc tactgcgtac tggcgcgaag
     5881 cgcgactaat ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac
     5941 tatcatttaa tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt
     6001 ttgaatctct tttcttatca ttcaggtacg agagtaggaa taatgaaaaa acaaacccag
     6061 ctgttgagtg cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtt
     6121 gcgtcgattc caggccaggt tgccgatcag gcccctctcc ccagtctggc cccaatgctg
     6181 gaaaaagtgc ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag
     6241 aaaatcccgg aagaattcaa aaagtttttt ggtgatgatt taccggatca accggcacaa
     6301 cccttcgaag gtttaggctc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgact
     6361 aacaaccatg tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag
     6421 tttgatgcaa aactgattgg tagcgatgac cagagcgata tcgccctgtt gcaaattcaa
     6481 aacccgagca aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat
     6541 tttgccgtgg cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt
     6601 tccgcattag gccgcagtgg gttgaatctt gaaggtctgg aaaactttat ccagacagat
     6661 gcttccatta accgcggtaa ctccggcggt gcactgttaa atcttaacgg tgagttaatt
     6721 ggcatcaaca cggcaatcct tgcgcctggt ggcgggagcg tcgggattgg atttgccatc
     6781 cccagtaata tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc
     6841 ggtctgttag gcatcaaagg cacagagatg agtgccgata tcgccaaagc cttcaacctt
     6901 gacgtgcagc gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcaaaagcg
     6961 ggcgtcaaag cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct
     7021 gagttgcgct ctcgtatcgc aaccaccgag ccgggcacga aagtgaagct tggcctgctg
     7081 cgtaacggca aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc
     7141 agcgctgaaa tgatcacgcc agcgcttgaa ggtgcaacgt tgagcgatgg tcagctaaaa
     7201 gatggcggca aaggtattaa gatcgatgag gttgtcaaag gaagcccagc tgctcaggca
     7261 ggcttgcaaa aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct
     7321 gaaatgcgta aagtgctggc ggcaaaaccg gccatcatcg ccctgcaaat tgtacgcggc
     7381 aatgaaagca tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gctgacgtgt
     7441 gatgtccggt taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca
     7501 tgtttgtgaa gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg
     7561 ttgccatgcc ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg
     7621 atgagacgcc tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg
     7681 tttacaaccg tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat
     7741 ccggtgtaat catggatcaa cgcggttata tcatcaccaa taaacacgtc atcaacgacg
     7801 ccgatcagat catcgtcgcc ttacaggatg gacgtgtttt tgaagcgtta ctggttggct
     7861 ctgactctct aaccgatctg gcggtgctga aaattaacgc cacaggcggt ttaccaacga
     7921 ttccgatcaa tccacgccgc gtaccgcaca ttggcgatgt ggttctggcg atcggtaacc
     7981 cgtacaacct cgggcagacc attacccagg ggattatcag tgccacgggt cgaatcggtc
     8041 tgaacccgac cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact
     8101 ctggcggcgc gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg
     8161 ataagagtaa cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag
     8221 cgaccaaaat tatggataaa ctgatccgtg atggccgtgt gatccgtggc tacattggca
     8281 ttggtggacg tgagatcgcc ccattacatg cgcagagtgg cggcatggat caactgcaag
     8341 ggatcgtggt taacgaagtg tcgcctgatg gcccagcggc aaatgcaggc atccaggtca
     8401 acgatctgat tatctctgtg aataacaaac ccgctatttc cgccctggag acaatggatc
     8461 aggtcgcgga aattcgccct ggttcggtga tcccggttgt cgtgatgcgt gacgataaac
     8521 agttaacatt gcaggtcacc attcaggaat atccggcgac caactaaggt cagcgtaa
//
LOCUS       DAZGAP010000194         8571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGAP010000194 DAZGAP010000000
VERSION     DAZGAP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.195"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            193..1323
                     /gene="ompC"
                     /locus_tag="ACHLAM_004356"
     CDS             193..1323
                     /gene="ompC"
                     /locus_tag="ACHLAM_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7855799.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVSDNNVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1435..2490
                     /gene="apbE"
                     /locus_tag="ACHLAM_004357"
     CDS             1435..2490
                     /gene="apbE"
                     /locus_tag="ACHLAM_004357"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO7855800.1"
                     /translation="MEISFTRVALLAAALFFVGCDQQPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            2564..3628
                     /gene="ada"
                     /locus_tag="ACHLAM_004358"
     CDS             2564..3628
                     /gene="ada"
                     /locus_tag="ACHLAM_004358"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO7855801.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3628..4278
                     /gene="alkB"
                     /locus_tag="ACHLAM_004359"
     CDS             3628..4278
                     /gene="alkB"
                     /locus_tag="ACHLAM_004359"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO7855802.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4354..5997
                     /gene="yojI"
                     /locus_tag="ACHLAM_004360"
     CDS             4354..5997
                     /gene="yojI"
                     /locus_tag="ACHLAM_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO7855803.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTRLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6215..7861
                     /gene="mqo"
                     /locus_tag="ACHLAM_004361"
     CDS             6215..7861
                     /gene="mqo"
                     /locus_tag="ACHLAM_004361"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO7855804.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8011..8520)
                     /gene="eco"
                     /locus_tag="ACHLAM_004362"
     CDS             complement(8011..8520)
                     /gene="eco"
                     /locus_tag="ACHLAM_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO7855805.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         2051 a         2141 c         2315 g         2064 t
ORIGIN      
        1 aagttccctt tcatttacat tctgaaacat ctatagcgat aaatgaaaca tcttaaaagt
       61 tttagtatca tattcgtgtt ggattattct gcatttttgg ggagaatgga cttgccgact
      121 gattaatgag ggttaatcag tatgcagtgg cataaaaaag caaataaagg catataacag
      181 agggttaata acatgaaagt taaagtactg tccctcctgg tcccagctct gctggtagca
      241 ggcgcagcaa acgctgctga agtttacaac aaagacggca acaaattaga tctgtacggt
      301 aaagtagacg gcctgcacta tttctctgac gacaagtctg tagatggcga ccagacctac
      361 atgcgtcttg gcttcaaagg tgaaactcag gttactgacc aactgaccgg ttacggccag
      421 tgggaatatc agatccaggg caacagcgct gaaaacgaaa acaactcctg gacccgtgtg
      481 gcattcgcag gtctgaaatt ccaggatgtg ggttctttcg actacggtcg taactacggc
      541 gttgtttatg acgtaacttc ctggaccgac gtcctgccag aattcggtgg cgacacttac
      601 ggttctgaca acttcatgca gcagcgtggt aacggcttcg cgacctaccg taacaccgac
      661 ttcttcggtc tggttgacgg cctgaacttt gctgttcagt accagggtaa aaacggtagc
      721 gtagatggcg aaggcatgac caacaacggt cgtggtgctc tgcgtcagaa cggcgacggc
      781 gttggcggtt ctatcactta tgattacgaa ggcttcggta tcggtgctgc agtgtccagc
      841 tccaaacgta ctgacgctca gaatggtacc tatgtaagcg acaataatgt ggtccgtaac
      901 tacatcggta ctggtgaccg tgctgaaacc tacactggtg gtctgaaata cgacgctaac
      961 aacatctacc tggctgctca gtacacccag acctacaacg caactcgcgt aggttccctg
     1021 ggttgggcga acaaagcaca gaacttcgaa gctgttgctc agtaccagtt cgacttcggt
     1081 ctgcgtccgt ccctggctta cctgcagtct aaaggtaaaa acctgggtgt catcaatggt
     1141 cgtaactacg acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac ctactacttc
     1201 aacaaaaaca tgtccaccta tgttgactac aaaatcaacc tgctggacga caaccagttc
     1261 actcgtgacg ctggcatcaa cactgataac atcgtagctc tgggtctggt ttaccagttc
     1321 taatctcgat tgatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta
     1381 caaacacagt tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa
     1441 ataagcttta cccgcgtggc actgctggct gccgcgctct tctttgttgg ttgcgatcaa
     1501 caaccacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg caaaaccatg
     1561 ggtaccttct ggcgtgccag catcccgggc attgacgcca aacgcagtgc cgaacttaaa
     1621 gaaaagattc agacccaact ggacgctgac gatcagctgc tttcgaccta taaaaaagat
     1681 tccgcgctga tgcgctttaa cgactcgcaa agtttgtcgc cgtggccggt aagtgaagcg
     1741 atggccgata tcgtcactac ctcgctgcgc attggcgcga agaccgatgg cgcgatggat
     1801 ataaccgtcg ggccgctggt gaatctgtgg ggctttggtc cggaacaaca accggttcaa
     1861 attccgagcc aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacctgacg
     1921 gtcattaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt atatgtcgat
     1981 ctctccaccg tcggcgaagg ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa
     2041 gggatttccc gctatctggt gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt
     2101 gctggcaagc cgtggcgggt agcgattcaa aagccaaccg ataaagaaaa cgcggttcag
     2161 gccgtggtgg atatcaacgg tcatgggatt agtacctctg gcagctaccg taactattac
     2221 gaactggacg gcaaacgtct ttctcatgtt atcgatccac aaaccgggcg tcctatcgaa
     2281 cacaatctgg tatccgtgac ggtgattgcc ccgacggctc tggaagccga tgcctgggat
     2341 actggcttaa tggtactcgg accggagaaa gccaaagagg ttgttcgccg ggaaggactg
     2401 gcggtctaca tgatcaccaa agagggcgat aattttaaaa cctggatgtc gccgcagttt
     2461 aaaagcttcc ttatcagcga aaaaaattaa agcgcaagat tgttggtttt tgtgcgatgg
     2521 tgaccgggca gactaaaggc tatccttaac cagggagctg attatgaaat atgccacatg
     2581 cttaactgac gatcaacgct ggcaatctgt cttagcccgc gactcgaatg ccgacggcga
     2641 attcgttttc gccgtgcgca ccacgggtat cttttgccgt ccgtcttgcc gagccagaca
     2701 tgccttgcgg gaaaatgtct ccttctacgc aaatgccagc gaggcgcttg ccgctggctt
     2761 tcgtccctgc aaacgttgtc agccagacaa agccaatccc ctgcaacatc gccttgataa
     2821 aattacccac gcctgccgac ttctggaaca ggaaacgcct gtaacgctgg aagccttagc
     2881 cgaccaggtg gcgatgagtc catttcattt gcatcggttg tttaaagcga ctaccggaat
     2941 gacgcctaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat tgctggcgaa
     3001 aggggagagc gtgactacat ctattattaa cgccggattc cccgacagca gcagttacta
     3061 ccgcaaagcc gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa
     3121 tctggcggtg cgttacgcgc tggctgattg tgagctgggc cgttgcctgg tggcagaaag
     3181 tgagcggggg atttgcgcga tattactggg cgatgatgac gcgacactaa tcagcgagtt
     3241 acagcagatg tttcccgctg ccgacagcgc gcctgctgat cttacgtttc agcaacatgt
     3301 gcgtgacgtg atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggacat
     3361 tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgcggtga
     3421 aaccgtcagt tatcagcaac tggctaatgc catcggcaaa ccgaaagcgg tacgggccgt
     3481 tgccagcgcc tgtgccgcca acaagctggc tatcgtaata ccctgtcatc gggtggtccg
     3541 tggtgatggc acactttccg gttaccgctg gggcgtgtcg cgcaaagcgc aactgctgcg
     3601 ccgcgaagct gaaaatgagg aaaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg
     3661 caagaaccac tggcggctgg agcggtgatt ttgcaccgtt ttgcttttaa cgctgcggag
     3721 caactgatcc gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca gatggtcact
     3781 ccaggcggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgacc
     3841 catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc
     3901 atgccgcaga gttttcatca tttatgtcaa cgtgcggcta cggcggcggg ctatccagat
     3961 ttccagccag atgcctgttt gatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat
     4021 caggataaag acgaaccgga tctgcgcgcg ccgattgttt ctgtttctct gggcttaccc
     4081 gcgatttttc aatttggcgg cctgaaacga aatgatccgc taaaaagatt gttgctggaa
     4141 catggcgatg tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg tattcaaccg
     4201 ttgaaagcgg ggtttcatcc actcaccacc gactgccgct acaacctgac attccgtcag
     4261 gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gcgcgcgaag atgtttaaac
     4321 tgcgggctgt aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg
     4381 cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc
     4441 attggcttaa ttgcttttat caatcagcgc cttatcgaaa cggcggatac cagtctgctg
     4501 gtattgccgg agtttctggg gttattgctg ctgttgatgg ccgtcactct cggatcgcaa
     4561 ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag
     4621 cggattctcg atactcacgt cgagcgcatt gaacaactcg gtagcgcctc gttgctggcg
     4681 ggattaacca gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc ggaactggtg
     4741 caggggatca ttctcactat cggttcagcg gcgtatttgt ggatgctgtc gggcaaaatg
     4801 ttgctggtaa cggctatctg gatggcagtc accatctggg gcggttttgt gctggtggcg
     4861 cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag ataagctgta cacggatttt
     4921 caaactgtac tcgaagggcg caaagagctg actctgaacc gtgaacgcgc cgagtatgtg
     4981 tttaacaacc tctacattcc tgatgcgcaa gagtatcgcc atcatattat tcgcgcagac
     5041 accttccatc ttagtgccgt gaactggtca aacatcatga tgctgggcgc tatcggcctg
     5101 gtgttctgga tggcgaacag tctcggttgg gctgatacca acgttgctgc gacctattcg
     5161 ttgacgctgt tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt gccgacgcgg
     5221 ctgacggcgc aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagca
     5281 gagtttccgc gcccgcaggc ttttcccaac tggcaaacgc tggagctgcg taacgtgacg
     5341 tttagctatc aggataacgc gttttccgtt gggccaatta acctcaccat caaacgtggc
     5401 gagctgctgt ttctgattgg tggcaacggt agtggaaaat cgacgctggc gatgttgctg
     5461 acgggcttgt accagccaca aaatggcgaa atcttgctgg atggcaaacc tgtcagcggc
     5521 gaacaaccgg aagattatcg aaaactgttt tcggcagtgt ttaccgatgt ctggctgttt
     5581 gatcaactgc tggggccgga ggggcaaccc gctaacccgc aactggttga gaagtggctg
     5641 gcgcagctga aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag
     5701 ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga agaacgcgat
     5761 attatcctgc tggatgaatg ggcggcggat caggatccac acttccgtcg tgagttttat
     5821 caggtgctgc tgccgctgat gcaggagatg ggtaaaacta ttttcgccat cagtcatgat
     5881 gatcattact ttatccacgc cgaccgcctg ctggaaatgc gcaacgggca acttagcgag
     5941 ctgacaggcg aagagcgcga tgctgcttcg cgtgatgccg ttgcccggac ggcataatat
     6001 tacgacgctg acctggcgga ctgctgccgc caggttaata tgctaaaaat cccttcattt
     6061 ctaataaccc tataattaat caacgaaatt ataatgtttc taaaattaga atataattta
     6121 taaacattat ttaaatgttg ttacttaagt gttaccgttg atgccgcgca aatctcacct
     6181 gcaataaagc gactaaaagt aaggcattaa caagatgaaa aaagtgactg ccatgctctt
     6241 ctcgatggcc gtggggctta atgccgtttc gatggcggca aaagcgaaag catccgagga
     6301 gcaggaaact gatgtactgt tgattggcgg cggcattatg agcgccacgt tggggaccta
     6361 tttacgcgag ctggagcctg aatggtcgat gaccatggtg gagcgcctgg agggtgtcgc
     6421 gcaggagagt tcgaacggct ggaacaacgc cggaaccggg cattctgcac tgatggaact
     6481 caattacact ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag tcgccattaa
     6541 cgaagcattt cagatttcgc gccagttctg ggcgcaccag gtcgagcgcg gcgtgctgcg
     6601 tactccgcgt tcatttatca ataccgttcc gcatatgagc tttgtctggg gcgaggataa
     6661 cgtcaatttc ctgcgcgccc gttacgccgc gttgcaacaa agctcgctgt ttcgcggtat
     6721 gcgttactct gaagatcacg cgcagatcaa agagtgggca ccgttagtga tggaagggcg
     6781 cgatccgcaa cagaaagtgg cagccacgcg tacggaaatt ggtactgatg tgaactatgg
     6841 cgagatcacc cgccagttaa ttgcttcctt gcagaagaaa tctaattttt cgctgcaact
     6901 cagcagcgaa gtccgcgccc taaagcgtaa tgacgataac acctggaccg tcactgttgc
     6961 cgatctgaaa aatggcactg cacagaacat ccgcgccaaa tttgtcttta tcggcgcggg
     7021 cggtgcggcg ctgaagctgt tacaggaatc ggggattccg gaagcgaaag actacgccgg
     7081 tttcccggtg ggcggacagt tccttgtttc ggaaaacccg gacgtggtta atcaccatct
     7141 ggcgaaggtt tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga
     7201 tacccgtgtt ctggacggta aacgcgtcgt gctgtttggg ccatttgcca ccttctcaac
     7261 caaattcctc aaaaacggtt cattgtggga tctgatgagt tccaccacca cctctaacgt
     7321 gatgccgatg atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca
     7381 ggtgatgttg agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaggcgaa
     7441 aaaagaggac tggcgtttgt ggcaagcggg gcagcgcgtg cagattatca agcgtgatgc
     7501 cgagaaaggt ggcgtactgc gtctgggtac cgaagtggtc agcgaccaac aaggaaccat
     7561 tgccgcgctg ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttggatct
     7621 gctggaaaaa gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc
     7681 gatcgttccg tcttatggac gcaagctgaa cggtgatgta gcggcaacag aacgcgagtt
     7741 gcagtacacc agcgaagtgc tggggctgaa atacgacaaa ccgcaggcag cagatagtac
     7801 gccgaaaccg cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgtg
     7861 atgatgtgcc acatccggca tggtatgccg gatgtggcgt atgcagataa gacgcgtcag
     7921 cgtcgcatca ggcaaccggc tcgggcgtta gatgtcggat gcggcgtaac cgccttatcc
     7981 gacctggcgg ttgcagcact tcacagcagt ttagcgaact accgcgttgt caattttctc
     8041 ttccgccttc cagacgcggt acttcacatc tacattgtct ggcgtataca ccacgatcgg
     8101 cagcttgctg ttataacgca gcattccggc atcgcccaga tacgcggtga caaatttctt
     8161 ctctttcttg ccatccgggc aggccatcat cgtcgaaacc ggggaactga ctttatcaaa
     8221 gacataataa tcatagcccc agccttccag cgttttgctt tccagcttcc cgccgagacg
     8281 atgcaaattg caatcgactt ccagcgtctg gccgattaac agttctactt tcagggtaga
     8341 ttcatcttct tgcggggtta actgaatcac ctggcgcttc atgccttttt cagcttgtgg
     8401 ataaggtgcg attttttcca gtggctggac gctttctgcc gcccaggcgg aagtggtggc
     8461 gaaagcggca aacaatactg caggtagaat ggtcttcatt tttttgcttt ccttcttcat
     8521 ttataggttt tcaccgatga tgtcgctgaa ggttaacata ttcatcagat t
//
LOCUS       DAZGAP010000195         8453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGAP010000195 DAZGAP010000000
VERSION     DAZGAP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.196"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..1230
                     /locus_tag="ACHLAM_004363"
     CDS             10..1230
                     /locus_tag="ACHLAM_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HFO7855806.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            1498..3648
                     /gene="btsT"
                     /locus_tag="ACHLAM_004364"
     CDS             1498..3648
                     /gene="btsT"
                     /locus_tag="ACHLAM_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO7855807.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            3698..3901
                     /gene="yjiX"
                     /locus_tag="ACHLAM_004365"
     CDS             3698..3901
                     /gene="yjiX"
                     /locus_tag="ACHLAM_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7855808.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            3912..4868
                     /gene="yjiA"
                     /locus_tag="ACHLAM_004366"
     CDS             3912..4868
                     /gene="yjiA"
                     /locus_tag="ACHLAM_004366"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7855809.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEVEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPSRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDKIRAAFAGLRK"
     gene            complement(4902..5204)
                     /locus_tag="ACHLAM_004367"
     CDS             complement(4902..5204)
                     /locus_tag="ACHLAM_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFO7855810.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(5219..5320)
                     /locus_tag="ACHLAM_004368"
                     /pseudo
     CDS             complement(5219..5320)
                     /locus_tag="ACHLAM_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            5517..6683
                     /locus_tag="ACHLAM_004369"
     CDS             5517..6683
                     /locus_tag="ACHLAM_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7855811.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVDREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            6747..8366
                     /locus_tag="ACHLAM_004370"
     CDS             6747..8366
                     /locus_tag="ACHLAM_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO7855812.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEVLGYGE"
BASE COUNT         2024 a         2208 c         2230 g         1991 t
ORIGIN      
        1 gtaaatgtta tgaaatacca ctcagaacct ctggttcccc ataaatctgc cctgatgcaa
       61 atgcccgcaa atctccttgc cgaagatgtc tgtttacctg ccgcgatcat taaaaagcag
      121 gccctggaga acaacattac gtggatgcaa cgctacgctg acgcgcgcgg cgtttcactg
      181 gcaccgcacg gtaaaaccac catgacgccg tggatttttc aggcgcagca ggcggctggt
      241 gcctgggcaa taggtgtcgg tagcgcatgg caggctggtg cggcaatggc gagcggtatt
      301 cagcgagtgc tgatggttaa ccagctggtc ggcaaggcga atatgcagtt gattgcccag
      361 ttgcaacgac attacccgac ggtcgatttt atctgctgta tcgacagcat tgagaacgcc
      421 cgcgctttgt ctgccttttt tgccagtcag caacagacac tgaacgtgat gattgagttg
      481 ggtgtccccg gcgggcgctg tggttgccgc tcaagggatg ccgcactgac actggctaaa
      541 caggcagccc aattaccggg gttacgcctg cgggggcttg agttatatga aggcgtttta
      601 cacggtgacg atccacaacc gcaggtagaa gctctgctgc gggatgccgc acaactggcc
      661 tgtgatatgg caagtctggt tgatggcgag tttattctca ccggagcagg cacggtctgg
      721 tatgacgtgg tgtgcaatat ctggctggcg gcagaaaagc ctgataactg ccgcattgtt
      781 attcgccctg gctgctacat cactcacgac accgggatct acgacgcggc acaacaacag
      841 ttgcttgccc gtgatcctgt ggcctgcgat ctggcaggcg atctcacatc tgcgctggaa
      901 ctggtcgcca tggtgcaatc ggtgccggaa gctgatcgtg cggtggtaaa ttttggtaag
      961 cgtgattgcg ccttcgacgc cggactgccg caaccagtcg cccactaccg caacggcaaa
     1021 tcactggcat ttgatccaca ggcgattcgc agtacaggta ttatggatca gcactgcatg
     1081 ttgcaactgt cagctaatag cgacgtgcaa gtgggggata ttctggtgtt tggcacatcg
     1141 catccgtgcc tgaccttcga caaatggaaa acgttgttat tgactgatga cgactacaac
     1201 gtactggcag aattagacac tctcttctaa gaacgcccct tcggttaatt atcgaagggg
     1261 catttttatt gtgatctcaa tcacatatcc acccgacgaa taattcttgc ccaaccgctc
     1321 accgccattc ctgaccgctt acatccctaa aataaccact cagttattta ccttacttta
     1381 cgcgcgcgta actctggcaa catcactgca ggatagcggt caatttacct cctcaaacgc
     1441 aacgcaaacc tagaacggct tcggccaact attaatcaat acatgccagg ttttactatg
     1501 gatactaaaa agatattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc
     1561 tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca gtgccctgtg gatcgtggtc
     1621 gcctctgtgt cggtgtatct ggtggcttat cgctactaca gtctgtacat cgcccagaag
     1681 gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac
     1741 tatgttccga ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct
     1801 ggtccgctgg ttggtccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg
     1861 ctgctggcgg gggtagtgct ggccggtgcg gtacaggact ttatggtgct gtttatctcc
     1921 tctcgccgta acggcgcatc tctcggtgag atgatcaaag aagagatggg accagtaccg
     1981 gggactatcg cgctgtttgg ctgtttctta atcatgatca tcatccttgc cgtcctggcg
     2041 ctgattgtgg ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc
     2101 gtaccaattg cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt
     2161 gaagtctctg tcattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt
     2221 gctcacgatc cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg
     2281 ctgattggct atgcgtttgt ttccgcgctg ctgccggtgt ggctgatcct cgcaccacgc
     2341 gactatctgg caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg
     2401 gtgctgaacc cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg
     2461 ctgtggaaag gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct
     2521 ggcttccacg cgctgatctc ttccggtacg acgccgaaac tgctggctaa cgaaaccgac
     2581 gcgcgtttca ttggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg
     2641 gttgctgcgt ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgctggc
     2701 cttggcatca ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg
     2761 gcgcagttga aagacgttac cgcacacgcg gcggcgaccg tcagctcctg gggcttcgtg
     2821 atttcgccag agcagatcct gcaaaccgcg aaagacattg gtgaaccttc tgtcctgaac
     2881 cgtgcaggtg gtgcgccgac gctggcggta ggtatcgcgc acgtgttcca caaagtgctg
     2941 ccgatggctg acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc
     3001 ctgaccgcgc tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt
     3061 aacttcatcc cgttcctgaa aaaaaccgac tctctggttg ctggtatcat cggtactgcg
     3121 ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc
     3181 gttaagagcc tgtggccgct gttcggtatt tccaaccaga tgctggcagc cgtagcactg
     3241 gtactgggca ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt
     3301 gttccggctg tatggctgct tatctgcacc acctgggcgc tgggcctgaa actgttcagc
     3361 accaacccgc agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct
     3421 aacggtactg acctgacggc acagcagatt gccaacatga accacatcgt tgtgaacaac
     3481 tacactaacg cgggtctgag tattctgttc ctgattgtgg tgtacagcat catcttctac
     3541 ggtttcaaaa cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa agaaactccg
     3601 tacgttccaa tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc
     3661 ccgcttcggc ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc
     3721 aaaaaaatat ctcggccagg cggcgaagat gttgattggc attccagact acgacaacta
     3781 cgtcgagcat atgaagacca accatcccga caagccgtac atgagctatg aagaattctt
     3841 ccgcgaacgc cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta
     3901 atggagagag catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggca
     3961 aaaccaccct gctgcgccat attcttaatg aacagcacgg ctacaaaatt gccgtgattg
     4021 aaaacgaatt cggcgaagtc tccgttgatg atcaactgat tggcgaccgc gccacgcaga
     4081 tcaaaacgct gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaggacgcgc
     4141 tactcgacct gctggacaat ctcgacaagg gcaatattca gttcgaccgt ctggtcattg
     4201 aatgcaccgg catggccgat cccggcccga ttattcagac ctttttctcc catgagattt
     4261 tatgccagcg ttacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg
     4321 agcagatgaa ccagttcacc atcgcccagt cccaggtggg ctacgctgac cgtattctgc
     4381 tgactaaaac cgacgtcgca ggcgaagtag agaaactgcg tgaacgcctg gcgcgcatca
     4441 acgcccgcgc accggtctac accgtcaccc acggtgacat cgacctgggc ctgttgttca
     4501 acaccaacgg ttttatgctg gaagaaaacg tcgtcagcac caaaccgcgt ttccacttta
     4561 tcgcggataa acaaaacgat atttcgtcga ttgtggtgga actggattac ccggtagata
     4621 tcagcgaagt ttcccgcgtg atggaaaacc tgctgctgga atcggcggat aaactactgc
     4681 gttacaaagg gatgctgtgg attgacggcg aacctagccg cctgctgttc cagggcgtcc
     4741 aacgcctcta cagcgccgac tgggaccgcc cgtggggcga tgaaacaccg catagtacaa
     4801 tggtgtttat cgggattcag ctgccggagg ataagattcg ggcggcgttt gcggggttga
     4861 ggaagtaatt ttagatgatg gccgccatgt ggcggcctga attatttttt attctgatcg
     4921 cgcagcatcg cttcacgtaa aattgcgttc aggcgagttt gatagccttt acctggtcgc
     4981 ttcaaccatt ccatcacatc agcatcaata cgcaccgaag cctgagtttt caatggccga
     5041 taaaacttac cgcgttctgc gttagaccat tgctcatcgc tggaaggtgg gatatcactg
     5101 taatcaatat cttcgtcgga tttattcgcc agcgccttca gttcggcctc atgctgcaca
     5161 tcgagtgtag aggaggtccc tcgtttatgt ttaaccatgc tcatagcgat tccgttcgtt
     5221 gtggatcatc aaacactaac accgcatctt caaagcggat gccatgtttc acccgattac
     5281 ctttggcctt atttgcatcc cattcaaact ccattggcat agttgccatc cctaattgta
     5341 tatacaaaaa tgtatgtaca attattctgc aattatagcg ttgatgttgg aaatagcatc
     5401 acgtttcgaa taatcaacca tgaaattgac aatgtgtgag ttggacttga ttcatccaat
     5461 agaatttctc gcctgacatg gcacactaaa cacctcgctg tcgttaaggg attaccgtga
     5521 aaaactacaa agtcattacc ccgctttttc ctacctatgc gcaagttaag gctatgatga
     5581 aagccgtctc aggctattcc ctgaaagcgg tgcgcaatat gattactgcc attcacgagc
     5641 aaaccggaac gccgcaaaaa cccgttgact ggtcggaacc tgacctgtgg atcagcgaac
     5701 gcttaacggg tgaagatgcc gacatcgccc gtcgtatttg ggataccgat aatcacatat
     5761 tgaacccgcg ccacagctac ggctgctact tattcctcaa ctatccccag tttgagctga
     5821 tggaaagcac gccagacgat acctggcagc ccacctcgcg cgggcaaaaa ttcctgcaag
     5881 atgacgaaaa aacgctgcgt tcactggacg atcaggaagg cattctgcaa cttcttgaac
     5941 tattagctgg ccgcgaaatg tcgcgccgcg ccgatttatt gccggaatgg caggcctttt
     6001 tgcatcagca ttcgaaattc gcgtctgcaa gttcagtgaa aagtacgctc tactcccgtc
     6061 tctataacct tatcgaccgt gacatggttg accgtgaagg catgagctat cgcatcaccg
     6121 ataccgggcg cgcctggctt ggaaaagcgt tgccggcacg aaaaagcgat ccacgtaaag
     6181 agctgctgga agcggtgaaa cgctacaacg agcagcaaaa agagctgctg cgcgaacaaa
     6241 tcagcacgat gaacccgtac aaatttgaac aactggttgg tcaattactg gaagcaatgg
     6301 gctacgagca ggtcgaagtg accaaagcct ctggcgacaa aggtgtggac gtgattggcc
     6361 aggtgcaggt cggcatcacc actattaccg aagtggtgca ggtcaaaaga atgcagaaca
     6421 ctatcactcg cccatatatc gaccagttgc gcggggcgtt gccttaccac aaagccattc
     6481 gcggtacact catcaccacc gggaaatttg cggcgaaatg tgcagaagcg gcgctctttc
     6541 cgggtgccgc gcccattacg cttatcgacg gcgatcgttt actggaactg ttaatcgaaa
     6601 ataacgtcgg cattcgccgc agcaatgcag tggaattgct ggatgtggat ttgcagttgt
     6661 ttgatgagct ggagatggat taaagggcga aatccgccgc ctctaccatt acaatagcga
     6721 cccaatttat ctgaacagag ttcatcatgg ccaaagcacc aactaaaaaa gccaaagcaa
     6781 agaaaggctt tgaagaaaca ttatgggata cagcaaacca gcttcgcggt agcgttgagt
     6841 cctccgaata caagcacgtg gtgttgagcc tcgtgttcct gaaattcatc agcgataagt
     6901 ttgaagcacg ccgcaaaaaa atgattgccg atgggcaagc agacttcctt gagatggaag
     6961 tgttctacca gcaggacaac attttctacc tgccggaaga agcgcgctgg tcttttatca
     7021 aacaaaatgc caaacaggac gatattgcgg tccgtattga caccgccctc tcgaccatag
     7081 agaaacgtaa cccaacgctg aaaggcgcgc tgccagacaa ctacttcagc cgtcagaatc
     7141 tggaaaccaa aaaactggcg tcactgattg ataccatcga caacatcgaa acgctggcac
     7201 acgaaactga cgttgaaacg ttatcgaaag aagacctggt cggacgcgtt tacgaatact
     7261 tcctcggtaa gtttgccgca actgaaggca aaggcggtgg tgagttctac acgcccaaat
     7321 gtgtggttac gctgttaact gaaatgctcg aacccttcca gggcaaaatt tatgacccgt
     7381 gctgcggctc ggcagggatg ttcgtgcagt cggtgaagtt tgtcgagagc catcagggta
     7441 aaagtcgtga tatcgcgctg tatggtcagg aactgacagc cacgacgtat aaactggcaa
     7501 aaatgaacct cgccattcgc ggtctttcag ctaacctcgg cgaacgaccg gcagacacct
     7561 tctttagcga ccagcacccg gacctgaaag ccgactacat tctggcgaac ccgcccttca
     7621 acctgaaaga ctggcgtaat gaagccgaat taaccaaaga cccacgtttt gctgggtatc
     7681 gtatgccgcc aaccggtaac gccaactacg gctggatttt gcatatgctc tccaagctgt
     7741 cggctaacgg cacagcaggt tttgtgctgg caaacggttc gatgagttct aacaccagcg
     7801 gtgaaggcga gatccgtgca cagatgatcg aaaatgatct gattgactgt atgatcgccc
     7861 tacctggaca gttgttctac accacgcaga tcccggtgtg tttatggttt atgaccaaat
     7921 cgaaggctgc cgatccggcc aaaggttatc gtgatcgtca gggcgagacg ctgtttattg
     7981 atgcgcgtaa cctcggcacc atgattagcc gcacaactaa agagttaacg gcggaagata
     8041 ttgccaccat cgccgatacc taccatgcat ggcgcagtac gccggaagaa ctggctgcac
     8101 ggattgcgcg tggtgacagc aagctggaaa aatatgaaga ccaggctggc ttctgcaaag
     8161 ttgcgaccct gcaagacatt aaagataacg actacgttct gacaccgggc cgctatgtag
     8221 gtgcagccga gcaggaagag gacggcgtgg catttgagac gaaaatgcgc gaattgtcga
     8281 agacgttgtt tgagcagatg aagcaggcgg aagaactgga tcgtgcgatt cgccagaatc
     8341 tggaggtgct gggttatggg gagtaagtgg atagaagtat cactgggcga gatttctgaa
     8401 aaaattggag atggaatcca cggaacgcca acttataaca attcaggtaa cta
//
LOCUS       DAZGAP010000196         8445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGAP010000196 DAZGAP010000000
VERSION     DAZGAP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.197"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2861)
                     /locus_tag="ACHLAM_004371"
     CDS             complement(<1..2861)
                     /locus_tag="ACHLAM_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO7855813.1"
                     /translation="MIIHFTLNGTPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNC
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTAL"
     gene            complement(2858..3637)
                     /gene="ygfM"
                     /locus_tag="ACHLAM_004372"
     CDS             complement(2858..3637)
                     /gene="ygfM"
                     /locus_tag="ACHLAM_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO7855814.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQTLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEEYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGDALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3688..5016)
                     /gene="ssnA"
                     /locus_tag="ACHLAM_004373"
     CDS             complement(3688..5016)
                     /gene="ssnA"
                     /locus_tag="ACHLAM_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO7855815.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKGGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDDAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSHSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLCDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5019..8117)
                     /gene="ygfK"
                     /locus_tag="ACHLAM_004374"
     CDS             complement(5019..8117)
                     /gene="ygfK"
                     /locus_tag="ACHLAM_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO7855816.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSCKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPPELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTELKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDNEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         1964 a         2182 c         2210 g         2089 t
ORIGIN      
        1 tccagcgcgg tgagtatttt ctccggtgtg aaatgccatt cgcgtaacca gatgccgcag
       61 gcatcgtgaa ttgcggtagc aatcgccgga gcggcgccat ttacaccgat ttccgagatc
      121 gatttcgccc cgaatgggcc gactttatcg tcgctcggca ccagcacagc gcggaaatca
      181 cgcggaatgt caccaatttt cggtgcgccg taactgcgta aatcacgcgt taacgggtga
      241 ccttcggcgt cataaatgat ctcttcgctc atactgtggc cgatagctcg cagagtggca
      301 ccgtagattt gtcccaacgc taactctgga ttgaccggtg taccgcagtc cagcaaggcg
      361 tagaatttat ccaggcggat ttcacccgta cgcgtgttga cggcaacttc agcgaagttt
      421 gcgccatacg ggaaggcgaa atcaggcgtg atataactgc cagttcccac cagtgaacca
      481 aagccggtgc cggtttcgcc tttatgggct atctctccga aactaacttc gcctttcttg
      541 ccgcgtacga cgcccggcgt tgctagttgg acatctgcca ctggctcacc caacatttgc
      601 gcaccgtgga acagaatttt ctcccgcaga ttttctgctg ccaaacgcgc cgcgttaccc
      661 gagaagcaag taccggacga ggcatatgcg cctttatcaa acaacgcatg atcggtatca
      721 ccggagataa catgcacgtc ctgcggtggg cagtgcagca cttctgctgc cagtttcgtc
      781 accacagtat ccagaccagt accaatatcc gcaccgccag aatgaacgat aaaggtaccg
      841 tctgattcca gtttgatcat gcagttagcc tgatcgatat ctgggatccc tgatttctgc
      901 atgataatgg cgacgccacg accgatgtgc cagtcaccgt tttgtggttt tggtgaagac
      961 cattggatca tctcgcgacc ctgacgcagg atctcttcca gtgcgcagct ggcggcggaa
     1021 ggaacggagg tcggcgcttt accttcaccg attgcaccga gaattttcag ctcttgccct
     1081 tcgtgtaccc ggttacgttc gataatttcc agttggtcga tctgtaactg ttcagccagt
     1141 tccgctaatg ccatggtgat agcgaagtta cctttcggtg cgccataacc ctgataagca
     1201 ccatttgggc aaatgttgct gtagtaggtg gtgacctgga aatcgacgtt atcgcacgga
     1261 tataacggca gcgacagcgc cggtccgtta catggtacgg tgagtgagtg gttgccgtaa
     1321 gggccagtgt tggcgcggaa atccatcttc actgctgtca ggcgaccatc tcttttcgct
     1381 cccagtttga cggtgacttt cgcaacgtga cgagaggtgt tagcaataaa ctcttcttca
     1441 cgggtgtagc ggaacagtac cggacgcccg gtcacgcagg ttgcccaggc gcacacttct
     1501 tccagcagga tgtcctgttt agacccaaaa ccgccgccaa ctcgctcttt aatgacatga
     1561 actttatgtt gtttcatgcc cacgaggcgc gcgacctggc ggcgtaagtg ccatggtacc
     1621 tgggtggagg cgtggatcac cagacgatcg ccgtccatac gggtaaagca gatatgtgtt
     1681 tcagtcgggc actgctgtgc ctgcgttgag ttataggttc gctcaatgat cacatcggca
     1741 tcggcaaagc ctttgtccat atcaccaata tgaccatgaa tactggcagc gatatttttg
     1801 cgtgggcgag aaccgatcgg gaagttgatg atcatatgct cgccgcgctg ggctgcatga
     1861 cagttatctt cctccagagt atctggtgca ccagcaacat acaccaccgg ttcatcgtgc
     1921 acgacaggcg catcttccgc cattgcctcg tcaatcgaca ttaccggctt aagcacttca
     1981 tattcaacgt cgatgagctt cagtgcttcg agcgcaatgt cttcactttc agcgacgacc
     2041 gcagcaacgc gatcgccgac gtgacgcatt ttcttgccga acattcggcg gtcaagcggt
     2101 gacggttccg gtgcgctctg accacccggg gtatagtaaa tatccgggca attcaggtga
     2161 gtaataacgt gaacgacgcc cggtaaggct tcagctttgc tgacatccag atgagtaatc
     2221 agtgcgtgag cgtgtgggct acgtaacatt ttaatgacgc aggcgtcagc cgttacgcga
     2281 tcttcaacat agcagggttt cgcctgcacc attttcgcgg catcagtttt aggataatgc
     2341 ttgccaatga cttcaaggtc gtcacggaaa gtcggagcga tatcaatggt ggcctgcgga
     2401 ttatttttac gtgcaaccgc cagttcaatg acctgatagt attgctgcca gccagcatcg
     2461 cggctaaaca gacctgagag cgcgtcgtcg atctcttcgc gggtaggtgc gtcgatgcga
     2521 tcgagaagat cggtgataat cagagccgca gctggatcgt tataaccaga ctgcaccacg
     2581 ccaacatcaa ccatggcctg ttgaaccaga cttaactcat tccatttgcc cagagattct
     2641 gcggtacgaa tatctgcctt ctctaactgt gcggcgataa gcaaggacgc gttaacgata
     2701 ttaccgttaa agataattgc gtcagaaccg gcaaacccaa agccatcatc gctgttgcgt
     2761 acagagtgca ttcccatgtt aaacaacagc ttttgcacgt tttcaccagg attaacggtt
     2821 agctcctggg gcgtgccatt taaagtaaag tggatgatca tacggcttcc tccccagcct
     2881 gttggcagtc ggcataaagg tcggctacca gaactcccgt gatatagcgt ttataggcca
     2941 cgctgccccg caaatcttcc tgcgggaaaa tggcgttggc gacagcctgt tcaagggcat
     3001 caccttccag gttttgtttt tcaacatcgt gcagacgcag tgctttactg gctacgccat
     3061 ccagcgcaat tcgcataccg tcgtggtctg ttattgcaac ggctgccgtc acgacggtta
     3121 aacctgcctg agaacggcta attttgcgag tcgcacaggt gcgatacgga tctttaatga
     3181 tgatttcggt tagcaggcga tcgcatgggc aggccaggta ctcctcaatt gacagagttt
     3241 cgccgttgcc aaaaaccagt tcagcatcca gtgccagcag gacgggaagc agcactgact
     3301 cttcttgccg ggcggcaatt tcaccaccaa tggtcgactg attacgaaca tggcgtgagt
     3361 aaacaaaacc gagagcttca cgcagtgctg caggaataaa tcgcgcatca cgcagtgttt
     3421 gcaagcgaga catcgcgcca atccgcagtg caccgttatc ccagtcaacc cagtccagtt
     3481 ccagatcctg taaggaaatg gcaatctttt tatcggtacg ggttggtgta gcgttgagtt
     3541 tgctgccccc ggcgaaccag acggcttcat cctggtagcg gcgcttcagt tccagcgcct
     3601 gttcgacaga gtcgggtctg aaaaattgtt caatcatcgt ctttccttaa tttcccctct
     3661 tctctgaaag aagaggggag ctgttattta tgccagcgca tccatccgac gccacatact
     3721 ggcagcggct tttctggctt gcgcataaat ggaatcgcaa tcgaagttaa actgacggtc
     3781 ttcatagacc atcacaccat tgaccatcac gctgtgaacg ctgcctgagc ccataccgaa
     3841 agcgatatgc ccggcgatat tgtctgccag cagcggcgtc ggcgagttgt aatcgcaaat
     3901 ggttaaatcg gctttgtaac cggcttccag aaggccaaat ttcgcgccaa agttgcggct
     3961 catcagttcg ttgccgttag ccagagcttt ggcaaagctg tcaggccaca gcggaccacc
     4021 cgcatcgcga tgtttaaaga aggcaaattt catctcttca aacatatctg aaccaatacc
     4081 gtccgtaccc aacgccagat tgcggatgtc gcaaagatga tggttgtagc cgacatggtt
     4141 gttcatgttt gaacgggcgt tatgcaccag gaatgcatca cgctgattga gcaacgcgat
     4201 gtcgtcttta gacagataaa gcccatgagc gaccagcgtt ttgctgtcga tgagatcgaa
     4261 ttgcgctaat cgtgccagca gatctttgcc gtaccagtga tgactatggg aaacatcgta
     4321 aaggtcttcc gcagcgtgaa tatgtaaacc acggcctgtg gctttcacgg cctcacgcag
     4381 catttccaga ccggcatccg gcacagtaaa cggcgcgtga gcaccaatat gagcctctac
     4441 cagatacggt tcagttgccg ctttcttcgc atcatcaatc tgacgggcga agcggatgtt
     4501 ttcttctaca ccttcctgca actctttgat accgttgtta cggtcagtag tttcaaaaca
     4561 ggtcatcgcg cgcaggccaa cttttaaaaa tgcgttgcgc aatgtggaga gcgacccgtc
     4621 gatatacgcc ggagaggcat ggtgatcgat aaccgatgta catccgccct taatcgcttc
     4681 cagggaacaa atcagtccgc tgtaatagag cgactcttca tcaagggcgc gatcgagtcg
     4741 ccaccagaga tttttcagcg ttgagatgaa atccgggcag ggggcgatgt ttgccataat
     4801 tccgcgggac agccccgagt aaaaatggtt gtgcgagcag acaattcctg gcatcacaat
     4861 ccgaccatgc atctctttgt agctggcatc ggggtagcgt tgcgtcaggg catcgccgat
     4921 agcgacaatc acatcatttt cgatggcgat atcaacgcct tcctgcactt tcgctgggtg
     4981 taactgcact gcggtgacat tcttcagaat caacatgatt acacctccac acggcccagc
     5041 agataatgat gatgctggtg gacatggcta atgatgcggc acatatcgtt cagctccggt
     5101 ggtacgttgt tgaactgacc ttcgttgtcg atgtttaaca cccagctttg gttattcaga
     5161 cgtacgcgta cccggcaatc ttccacaagg aagcctgggt tgctgctgtt atcaaagtct
     5221 tgcgacaggc tgaagacggt gattttgtct ttgtacggtt taccgttcca cgggcagaat
     5281 tgggcgcagt tgccacattc gttacagtaa gcgtcgaggt gcagcgtctg gaaacggttc
     5341 tggaagcctg ggaccgcaat ggatacgttg gcgcggttcg ggcagacatc cacacacttg
     5401 ctgcaaacgt agttacattc aaggcagcgt gcggcttcct gggcgacaaa cgcgtcacga
     5461 tcgtcgctat ccaccagggt gacagagata tcgcctttgc gttgatagat ttccgccgga
     5521 ttaacgttgt tccagtattt atcgttctgg tgggaacgga tattttcccg actaaggatg
     5581 gcatcggtcg cccgacgcgc ggttccgaca gcagcgacaa tggaggatgg tccgcgctgt
     5641 acgtcgccga tcataaagac gtcagtcaga cgagtttcgc cattatggtc gacgtctggc
     5701 cagccgtttt tgtccagcgg cacgcccatc gcattcaggg cttcagtatc ctgctgttca
     5761 ccaatggcgg tgatcaggct gtctacatgc agtgttactg tttcgttggt ttcaaccgga
     5821 cggcgacgac ctttctcatc aggttcgcca agcgacataa cgcgcaaggt taaggtgcca
     5881 tcagcatcga aacgttccgg attattcaga aaacggaact ccacgccgtc gtgcaacgct
     5941 tcttcatact cttcgcgcca tgctggcatc tcttgcagtg aacgacggta aacgaccgtt
     6001 gctttttcta cgcctggaac gcgtaacgcc gcacgagcgc agtccattgc ggtgttaccc
     6061 gccccgacaa cgaccacatg tttgcccagc ttgagctctg tgcccttgtt gtattcacgc
     6121 aggaagggga gtgatttcca gacattttgg ttgtcgcccg ccagtttcac accgctattt
     6181 ttatcagtgc cggtggcaat cagaacatag tggaagccct gattttttaa ctgttcaacg
     6241 gtcagatcgg gtgagcagcc atactcaaat ttcacgccgt gagcggcaac aaaatcgata
     6301 tcgtgctgaa ttaactctgg aggaatacgg aactgaggaa tgatattttt caccacgccg
     6361 cccgcgttgg cttcgcgttc aaacagcgta accggatggc ccgctctggc aaggaagtaa
     6421 cctgcggcca gacccgccgg acctgcacca atcacggcaa ccggatggcg tgaaccagaa
     6481 ccggctggtt tgtgccagcg ttgcttatat tcatcccaac ctttttccag cgcgactttt
     6541 ttcagttcgc ggatattcag cgcactgtcg taatccaggc gggtacagtt gtattggcac
     6601 tggtgatcgc aaatatgacc ggtaatggcg ggcagggcgt tgcgttgata gatgagttcc
     6661 agcgcgtcgg catagcggtg ttcgccaagc agacggatgt attccggaat atcttgcttg
     6721 atagcgcagg cagtaacaca gggggcaacg tagcaatcgg tcagcggcag gtcttcagcc
     6781 acttcaatac gctcttctgg cttccagtgt ttctgggtgt attccatggt taacgcgtct
     6841 gctgccagtc tgttcagtcg ttcgacgtca acgtggtcaa gtccccaggc gtcggagcct
     6901 tccagctcgc gcatgcaggc acttaagcgc agatagccgc caggtttcag caggtcggtt
     6961 gccatagtaa tagggcgaat accagtatca aaaatatcgc ggatagtcag ctgactggca
     7021 ccaccggaat aagaaatggg cagtttgccg tcaaaggcgc gagagagaac tgctgcaacg
     7081 ttgatggaga gcgggaacag cgcacggcct gacatataca tctcttcacc gggcagtgcg
     7141 cctttgttat taatggtgcc gagagtgtta gtcagtttta cgccaaagcc gagtgatttt
     7201 tcttttgcca gtgccatcag gcgttccagc atttccagtg cttgcgtcag cttgaggtcg
     7261 tgatcaaatg actcttcttt taagccgatg taaccgaaac cgcagacatc gaggatctca
     7321 cgaacacgcg cgtaccccag taaggtcggg ttgagtttca caaaggtgtt gagccctttt
     7381 tcttccagca tgtagcggca aatggcttcg atttcatgcg gaggacagcc gtgcatggtg
     7441 gagagggtaa ccccttgtac catgctggtg gggatgcgag cgggtaaggc ttgcaagctt
     7501 tcgcgttttt cctgtaagcc gtggcgggct aaaaatgcgt catcctggag tagcttatta
     7561 agtgcatcgc ggtattgagc gaatttcgga tggtcagatg cgtccatcat attgtcgatg
     7621 aactgctgca tcggcggttg cttaatacct tcgaggttgt aaccgacgct catattaaag
     7681 atgaacgatt tacaggaatc agaaggctgg aacatcgctt cgagaaggtg cagggcaaac
     7741 caggctttga ggtattcatc ccaggcttta agcagggtaa attcggtaga ccattcggtg
     7801 ttaaagcact cgtcttcggc atcgatacag ggcttttcca gctccaggcg gtcaagaatt
     7861 tggacggttt ttagttcgat gaagcgtccg ccagtcagcc aggaagtgac aatattttgc
     7921 gcgagctgcg tgtgcgggcc agcggcaggg ccgacgggag tggcacaggt ttcaccgaag
     7981 acgctaacag ttttaccttt tacgggtgag taaaactgtt gctcgggaat accaaagatt
     8041 gagcgttgtt gttggtattc atcaaatatg cgcgtcaaaa gttcctcaaa cggaatggga
     8101 cgcataatat cccccataac cctctcctta gcttatacag atatcattga gtgaccggtg
     8161 ttgagaaatc cggtggttta gggggataga gcaacaatcg caccactctt gccaggtttg
     8221 gcgggaaata taaaaaaatg tgaggcggct cgaaacttaa tggctttttg tgaatgaact
     8281 cacaggtgcc aaatgagaat taaaatcact cctgaaaatt attattgcca gcccatttat
     8341 cactttttct cttttctcaa aatcagtgat tccttatcat tatgataaag agtgctggag
     8401 ggattaaata tttgaacgca atcgtttaac tacgttttct aaatt
//
LOCUS       DAZGAP010000197         8397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGAP010000197 DAZGAP010000000
VERSION     DAZGAP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.198"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            312..599
                     /locus_tag="ACHLAM_004375"
     CDS             312..599
                     /locus_tag="ACHLAM_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855817.1"
                     /translation="MEKEIKFAPKDIDEELAKIGMLERMRDIIEYAIKENLAAREALL
                     IMEREINLIKDAVSLDNKIAREEYVRRRLGVDGSAILTSEHYAKIFNLFSR"
     gene            complement(580..819)
                     /locus_tag="ACHLAM_004376"
     CDS             complement(580..819)
                     /locus_tag="ACHLAM_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855818.1"
                     /translation="MSDIKNALDVAAFTMAGNLMVTLFDKGVISREEATHIINIAQKS
                     AAPQSAEGHQDGSYINTNWHFDNLRACMGITAKKD"
     gene            890..1402
                     /locus_tag="ACHLAM_004377"
     CDS             890..1402
                     /locus_tag="ACHLAM_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO7855819.1"
                     /translation="MNRASLITLIHVAKRDLQLDRETYISALLAATGKTSCRDMSPDE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWV
                     KSQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAV
                     CRQYEKSLPR"
     gene            1472..1897
                     /locus_tag="ACHLAM_004378"
     CDS             1472..1897
                     /locus_tag="ACHLAM_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO7855820.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            1969..2469
                     /locus_tag="ACHLAM_004379"
     CDS             1969..2469
                     /locus_tag="ACHLAM_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7855821.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFQAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            2504..2932
                     /locus_tag="ACHLAM_004380"
     CDS             2504..2932
                     /locus_tag="ACHLAM_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855822.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            2916..3134
                     /locus_tag="ACHLAM_004381"
     CDS             2916..3134
                     /locus_tag="ACHLAM_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855823.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            3145..3372
                     /locus_tag="ACHLAM_004382"
     CDS             3145..3372
                     /locus_tag="ACHLAM_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7855824.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            3353..3661
                     /locus_tag="ACHLAM_004383"
     CDS             3353..3661
                     /locus_tag="ACHLAM_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO7855825.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            3658..3948
                     /locus_tag="ACHLAM_004384"
     CDS             3658..3948
                     /locus_tag="ACHLAM_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO7855826.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRACVPGVKKPRARG"
     gene            3951..4532
                     /locus_tag="ACHLAM_004385"
     CDS             3951..4532
                     /locus_tag="ACHLAM_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO7855827.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            4532..6196
                     /locus_tag="ACHLAM_004386"
     CDS             4532..6196
                     /locus_tag="ACHLAM_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855828.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLTEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILPRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            6196..7785
                     /locus_tag="ACHLAM_004387"
     CDS             6196..7785
                     /locus_tag="ACHLAM_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO7855829.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            7769..>8397
                     /locus_tag="ACHLAM_004388"
     CDS             7769..>8397
                     /locus_tag="ACHLAM_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO7855830.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLS"
BASE COUNT         1912 a         2210 c         2491 g         1784 t
ORIGIN      
        1 gtgctgattg ttcagtatgg tcacaggcag gaactgcgca tcggccatat ggggtatgaa
       61 ggctttttta ttgatgaaga aactggagcc tgccttgaag atgaggacgg acaggtgacg
      121 cactggtgcc atatttcagc tttaccggaa ttacaggaga cgcccaatgg atgatcaaat
      181 agttgaatgt cccacctgtg gaaatgaagc cccggagtat ctgaaagagt gcccacattg
      241 tggtgaggta aaatgtaatc actgcgatat gggcgacgac actgcatgta tgaattgtga
      301 ggattaataa tatggaaaaa gaaatcaaat ttgcgcctaa agatatcgac gaagaactgg
      361 ctaaaatcgg catgcttgag cgtatgcggg acattatcga atatgcgata aaagaaaatc
      421 ttgcagccag agaagccctt ttgataatgg agcgggagat taacctgatt aaggatgctg
      481 tatctctgga taataaaata gcccgcgagg aatacgttcg ccgcaggctc ggtgttgatg
      541 gttcagcaat tcttacatct gaacattatg caaaaatctt taatcttttt tcgcggtaat
      601 gcccatgcag gcccggagat tgtcgaaatg ccagtttgta ttaatatatg agccatcctg
      661 gtgtccctct gcgctttggg gtgcggcact tttttgagca atattgataa tatgtgttgc
      721 ctcctcacgg gaaataaccc ccttatcgaa cagggttacc ataagatttc cagccattgt
      781 gaatgcggca acatcaaggg cattttttat atctgacata gatttttcct tttatgttaa
      841 ttgacatggc ggtgcggtaa caccgcccat tttttatgga gccacgataa tgaatcgcgc
      901 ttccctgatt accttaatac atgtcgcaaa acgcgatcta cagcttgacc gggagaccta
      961 tatatccgcg ctgctggcag ccaccggcaa aaccagctgc cgggatatgt caccggatga
     1021 gttatcccgc gtgctggatg ttttcaaaaa acgcggtttt aaagtgcgcc agaagccggt
     1081 taaccgggcc ttaaaaccgg gtacggtgac cgccaaaatt cgtgccatct ggaaggtgat
     1141 gcaccggcag ggctttatct ctgatggtgc ggaaaccgct cttaaccgct gggtgaaatc
     1201 gcagacggcc gcgcagaacg gcggcgaagg tgtggcaaac tggcagtggc tggagcaaca
     1261 ccccgccctg gcctcagatg tgctggagcg tctcaagcga tggcaccgcc gcaaaatgct
     1321 ggccgcgatg ggaatgcccg aacgcacgct gatggggtat gacgccgttt gcaggcagta
     1381 tgaaaaatca cttccccgtt aaccccaaat cccgccacaa cgcgggattt ttattttaaa
     1441 cttaccggga acgcgagaac cggaggctga tatggcagaa actcagatga gcatgtttgg
     1501 tggtgacagt gagcaactgc acgcccttat cgaccgcctt gatgacatcc ctgatgatgt
     1561 tctcaaaaag aactggccgc ggaccctgtc agaactggtt gaagtcaccg gcgcagaact
     1621 tcagcgtcag gggattgagc cggtactggc cggtaaactg gcacgcaagg tggctgcggc
     1681 tcaggccgcc tatatgggcg gacggggtta ttacctgccg gtcggggaat ctctctttgc
     1741 cgagctgcga aacaatgaga tattttcgcg ctgggaccgg ggtgagaaaa ttgaatccct
     1801 tcgccgccat tacaggatgt cagaaaccca gatttatacc gtcatacgcg aacagcgccg
     1861 tctgcatctg gcaagaacgc aaccgccact tttctgatat tccgcaaagc ctgccgcctt
     1921 tccttcaccg ttacgctgac tcagagaaca tcatgagaca gcgtaacaat gccaaaactc
     1981 cccgcaccac tgcgtaaaaa gctgattgcc cttgttctgg ccggtgccgg gacgttcacg
     2041 attgccacgc attacaccgg ttactgggaa gggaaagaaa actccacgta tatcgatccc
     2101 accggtacac ccaccatctg ttacggccat accggcccgg atgtgaaacc gggtatgacc
     2161 ctgacggatg aagaatgcct ggaactactg gaaaaggaca tgaaatgggc ctttgcggcc
     2221 attgatcggc gtgttcaggt gccgcttacc cgtggtcaga cggtggcgct ggcttcgtgg
     2281 atcttccagg ccggtgaaac gaactttcgc aactccacgc ttctgcgcct gatcaatgcc
     2341 gggcagatgc ccgcgtcctg taagcagtat atccgctgga tttattcaaa gggggtgaaa
     2401 ctccccggcc ttgaggcccg ccgttcggcg gatgaatggt tatgtcgcta cgacttgccg
     2461 aaagtctgaa ccgcttctgg cgaccgctca tgatagcgct gctgtgtgcg gtactgctgt
     2521 tacggggtgt cctgtggctg cggtgaataa tgccttaccg gccctgattg ccggggcgtg
     2581 catggcggcg ctggggatta tcgctgtact ggttgccgcg atatggggaa tgcaccagaa
     2641 gacgcagcgc cttgaggaca ataatcaggt gcttgtgcgt gagcgggatg aggcgcgtca
     2701 ggtgctggca aatcagcagc acaccctgca actcatttca caaatcagtg aggcggccac
     2761 gaatgaaaaa caacagaaca ttcagcacag cgaggggcag cagagcgttg tccgccggtc
     2821 gctggcagca gtgcctgcgg cctctgcccc tgttcctgat gatgtggctg atcgggtgcg
     2881 ccgggccgtc tgtgaaatac gtgcctgtga agccggtgcc gatccccgct gaatggctgg
     2941 ctgactgcct ggtccctcct gcgccggagc cgttcacgtt tggggcatcg gtcacttaca
     3001 acctgcaact gctggcggtg atcaagaact gcaacgtgga caaggccagt attcgtcgtc
     3061 tggaggcgcg gcgacaacat gaatttactg atatggccgg aacgcctgct gttccggcag
     3121 gaaagacgaa gtaaggaaaa aggtatggat gattcagatc gtgctcaggc tgtgatggag
     3181 cggggatcag aacgcgccct ttgtaaccgg ctgacacgta agcgccgggc aatggatgct
     3241 ccgggcagac gtgtctgcgc agactgtggc ggagagatcc ccgccgcacg tcttgttgct
     3301 gtgccggatg ccatccgttg tgtgaactgc cagaacatca tggaggcccg tcatgtgggt
     3361 cagcatcgtt aaagattatg ttgtgccgat cctttcggcg acggcaaccg ctggcgggat
     3421 attcatggcg ctgatgcgca aaacgtttgt cccccgcgag gcctttgaaa aactctccga
     3481 ccgcgttgag aaggtggaaa cccgcctgtc gtcactgccg acagaagccg aggtcaaccg
     3541 tctgaatgtg gaaatcgtga ccctgcgggg tgaactgaaa accacgaacg ccacgctccg
     3601 ctctgtctcc tatcagaacg aactgctgct ggagcaggct gtaaggaaaa aaacgcaatg
     3661 agtgatttca ttactgaaga tcagcgtctg gtcattctgc ggtcgctggc agattacaac
     3721 ggtgaactgg gtgaatccgt gctccaggac tgtctggatg attacggcca ccgcgtgtcc
     3781 cgcgacaccg ttcacaccca cattgcctgg cttgccgagc agggactggt gcgcaaacgc
     3841 gttctgatta acggttattt catcgcggaa ctgaccggtc gtgggcagga tgtggcggaa
     3901 ggccgggcct gcgttccggg tgtgaagaaa ccacgcgcaa gggggtaacg atggacaagc
     3961 cgacgcgtgg tcgtgtacgc aaggtggatt tgctccccga cagcatccgt aagccgctgc
     4021 tggaaatgct gcgtgaaaaa cgtctgacgc aggtccagat ccgcgaggaa atcaaccgtc
     4081 tgatccgtga ggcgggcctg ccggaagagc agcagctttc accggcggcc atcagccggg
     4141 aagcctcccg taatgagctg attgcccgta acctgcgtga tttacgcgag cagacaaaag
     4201 ccatgatggc cgaactgggg gacaagccaa ccggtgaaac cacagcgctg attctggaaa
     4261 tgtcccgcgc cctgatgtac cgccgcctgc gtgccgcgac tgaatctctg aacagcgaca
     4321 gcgacgtgga tatgcgcctc atcaaagaca tcctcctgtc agcacagcgt gcggaaagtg
     4381 ccgccgagcg cagcattaag cgtgaaaaag aaatccgggc cgcatttgct gaagagatgg
     4441 caaacgcggt caccgacgag ctgcgcggcg tggacgggat gagtgaacag cttgagtccc
     4501 gtattaaggg gattctgttg ggtaaagcct gatgaataac gccgaagaac tctcaccctt
     4561 actgacgaat accgtctcca cccgcaaaat tgatctggcc ggtgaaaaag ccctgctggg
     4621 cgtggatgtg ccggacagcc ttgatttgcc cggtgatatg ccggtgtttc tggattatca
     4681 ggcccgctgg tttgaggatg aaagcgaggt ctgcatcgcg gaaaaatccc gccgcaccgg
     4741 gcttacctgg gcggaagccg ggcgtaacgt cattaccgcc gcaaagccta agcggcgtgg
     4801 tggccgcaat gtgttttatg tggggtcaaa gcaggagatg gcgctggaat atatttctgc
     4861 ctgcgcactg ttctcccgtg cctttaacca gctggccgat gctgatgtgt atgagcaaac
     4921 cttctgggat cgggataaaa aagaagaaat tctgacctac atgatccgct ttccgaacag
     4981 tggattcaaa attcaggcac tgtcttcccg tccgtcaaac ctgcgcggcc ttcaggggga
     5041 tgtggtgatt gatgaagccg cgttccacga gtcactggat gagcttctca aggcggcaat
     5101 ggcgctcacc atgtggggcg cgcgcgtgcg tattatctcc acgcataacg gcgtcgataa
     5161 tctgtttaat cagtacattc aggaagcccg cgaggggcgc aaggattaca gcgtccaccg
     5221 cattaccctg gatgatgcca ttgcggacgg gctgtaccgt cgtatctgtt atgtcaccgg
     5281 ccgcgaatgg tcaccggaaa gcgagcagaa gtggcgtgat gatctctaca aaaacgcccc
     5341 gacccgtgag gatgccgacg aggaatacgg ctgtatcccg aaaaaatccg gcggtgccta
     5401 tattcctcac gcgcttattg aaatggcgat gatccgcgac atcccgattc tgacgtttga
     5461 agccccggac gacttcatca gccgtgccgc atggctgcgt gaatcggagg ttttaacctg
     5521 gtgtgaagaa catttaaaac cgcttacaga gaaattaaat ccccgttccc gcttcagctt
     5581 cggggaagat tttgcccgca ccggtgacct gtcctgcttt gtgctgctgg aaatcaccga
     5641 atccctggca aaacgcgagg tgtttcgcgt ggagctgcgt aacctgccat atgcccagca
     5701 ggagcaggtg atgatgtaca tcctgccccg cgttccggcg ctggtcggtg cggcgttcga
     5761 cgccaccggt aacggcggct atctggccga agccgcgctg ctggcctttg gcccggacat
     5821 catcgactgc gtgatgttat cgccgaagtg gtacggcgag tggatgccaa aactgaaggc
     5881 cgagtttgaa gatcagaata tcctcgtcgc ccgccatcag accacgcttg atgatttgcg
     5941 ccatgtgaag gtggtgaacg gtatcccgca aatcgacaag gggcgtacga aggaccagaa
     6001 cgcaacggct gtgaacgccc gccgccatgg cgattttgcc gtggcgctct gtatggcaaa
     6061 ccgggcgtca tacatggagg gcttcatcct ggatgaatca gcctgtcagg cgctgccgga
     6121 gcggtcgcgg gccatggagg gcggttatcg tgatgacgat gaggcatatc atgaatttga
     6181 tcgggggtgc tggtagtggg acgtataatt gatcttgacg gaaaaccttt ctcctttgac
     6241 ccggagatgc agagtgccgc gctggatatt ccgcagattg ccagccgtta tattgaacat
     6301 ccggcctcgg gtatcacccc gaaccgggcg gcacagtgcc ttcgcggggc tgaacgtggc
     6361 gatctgattg cccagtccga tctggccgct gacattgaag aaaaggatac ccaccttttt
     6421 gcagagctgg gcaagcgacg tcttgccatt cagggggtgc catggagtat tgagccgcca
     6481 ccgaacgcca gcgcgaatga gaaaaaggac gcggaaatgc tcgacgaata tctgcattcc
     6541 gccgactggt ttgatgccat gctgtttgat gccaccgatg ccatcctgaa aggctattcc
     6601 tgcatggaga ttgagcacgg gatgctcggt aaaatgcaca tcatccgcgc catccgctgg
     6661 cgtgacagcg ggcatttctg ccttaacccg gatgatttga gcgagctgcg gctgcgtgac
     6721 ggcagccatg ccggggtggc gtttcagccc tttggctgga tagtgcatca gtcgcgttca
     6781 cgcaccggct acggtggtgc gacggggctt gtcagaacgc ttatctggcc gttcattttc
     6841 aaaaattatt ccgtgcgcga tctggctgaa tttctggagg tgtacggcct gccgatgaag
     6901 gtcgggaaat acccgtccgg ggcaacaccg gagcagaaaa gcgccctgat gcgggcggtg
     6961 atggatatcg ggcgacgtac aggcgggatc atcccggccg ggatgtcgct ggagtttcag
     7021 gcggcagcga acggtcaggc cgatccgttt gaaaccatga tttcgtgggg ggagcgttcc
     7081 atctccaaag ctatcctcgg cggcacgctg accacggaag ccggagacaa gggcgcgcgc
     7141 tcgctgggtg aagtgcataa cgaggtgcgc cgggaaatca gggattctga tttacgtcag
     7201 ctggccgcca cgctgaaccg cgatctggtg tatccgctgt atgccctgaa caccgcccac
     7261 gccattgata tccgtcgcct gccgcgtatc tgtttccaga caaaagagcc gggggatatc
     7321 accaaaatca ccagtgcggt gatgcagctc agtacgggga tggatattcc tgatccctgg
     7381 gtacgggagc agaccggtat tccacagcct gcccccggtg aagccatctt ccgtgtccgt
     7441 cagagtggca atgaacctgc tcagacagac aaagagatgc caccggaaaa acaggagaag
     7501 acagagcaga cagcgctggc tgccagactg ccggaagcga agagcagccc ccgagatgaa
     7561 ctggacgaca tgggggatgc ggtgcctgcc cgccggttac aggaggccat cgacccggta
     7621 ctggagccgg tcattgaagc catcagaacg cgggggctgg cggaggcact ggcagacctg
     7681 cctgcccttt accgtgaaat ggatgattcc cgcctgatga cgctgctcag tgatgccatg
     7741 tttgctgcgg aaatgaaggg gatgctggat ggcacagggg attgatttag gttatgccgc
     7801 cacgcttccc tcaaaagagg cggtggcata cttccgcgcc aaaggggcgc atatcagctg
     7861 gaactggttc gaaacagacg cggatgttca tgcccgttca ttcacggcgg caaaagtggc
     7921 acgcctggac gtactgacca cactacaggc ggaagtgcag cgtgctgttg atgaggggat
     7981 ttcacagaaa gcatttatcc gcacactgac accccgcctg caaaagctgg gatggtgggg
     8041 aaagcagatt gtggtggaca gcgccggtaa cgcagaagaa gtgcaactgg gcagtccccg
     8101 ccgtctggcg ctgatttaca acgtgaacac ccgcgtggct tacaatgccg ggcgttacac
     8161 gcagatgatg aacaacacgg acacgcatcc gttctggcag tatgtggcgg tgatggacag
     8221 ccgcacccgc ccggcgcatt ccgcccttaa cgggctggta ttccgctatg acgatccgtt
     8281 ctggaataca cactacccgc ccaatggctg gaactgtcgc tgtcgtgtcc ggccattgtc
     8341 tcaggcccgt ctggatgcga tggggctgtc tgtttcatcc ggtcaggatc atctctc
//
LOCUS       DAZGAP010000198         8202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGAP010000198 DAZGAP010000000
VERSION     DAZGAP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.199"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..775
                     /locus_tag="ACHLAM_004389"
     CDS             <1..775
                     /locus_tag="ACHLAM_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855831.1"
                     /translation="QKSRLNNTVRYEKLSRKKGFNFWLSLQLIIPGVVQILPMMFLFN
                     QLNFSEGTSDWYEMSILIAITVVIVTIGILPGIIHINERQNGNKMTGIIVVLIFIPVA
                     TAALSVWYRPIPNMIANMTMNLSGISDQRTHEYYIERATHPAGMFNGKIWNTRYYKNI
                     PDRFFITGVNTFTLGNIKLICPTAIVKARIESLKFTVNDIDEYEQKGKKLKKTAMKCI
                     PFDKNDIHTWDSPLSEPIYYEKIKQTIDNSMLKILHVVK"
     gene            843..1151
                     /locus_tag="ACHLAM_004390"
     CDS             843..1151
                     /locus_tag="ACHLAM_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO7855832.1"
                     /translation="MQSQTALLLLIIPFPVLVIASPESTQLALALKQLDGVKTSLEHA
                     RTSASNDPQARYFFDYLQAQQDISVVEQGIRRYLAPARAQPRAIQPLSANYQQENPDD
                     "
     gene            1144..1377
                     /locus_tag="ACHLAM_004391"
     CDS             1144..1377
                     /locus_tag="ACHLAM_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO7855833.1"
                     /translation="MTDSQLAAFQAASLSKPETVSLLVLGLFTSVLLLWCAWTLMVAW
                     QGVCRQRLSWQSFGAVAAHSLLLVLVSFWLVLS"
     gene            1387..1704
                     /locus_tag="ACHLAM_004392"
     CDS             1387..1704
                     /locus_tag="ACHLAM_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO7855834.1"
                     /translation="MRWFLLLLFATFNALADLPTMEDPSRGQGKGIMETLQNYGFDIV
                     VLLALAICAVGFLVVANNCIATYSEIQSGRKQWKDLGSMAGVGSILLVVIIWLLNQAS
                     DVL"
     gene            1706..2059
                     /locus_tag="ACHLAM_004393"
     CDS             1706..2059
                     /locus_tag="ACHLAM_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO7855835.1"
                     /translation="MATIPFLPDRLNREPVVWRGLTTSELFLALALGLGGGCAFGILL
                     ALITHYWPLIPGSALAGAALLIQGGGRILARAKRGKPDTWLPQALLAWLERHHLRGAQ
                     LIQHSAVWVVRRSPR"
     gene            2056..2715
                     /locus_tag="ACHLAM_004394"
     CDS             2056..2715
                     /locus_tag="ACHLAM_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO7855836.1"
                     /translation="MSRFRHALAERDNHILTLRAVIVLLCLCLAGVGYGWYSAPQKLT
                     LWFPPDLRSGSTRPWWEVPPASVYAFTFYIFQQLNRWPDNGEEDYSRNLRSLRAYLTP
                     ACYTRLEHDFQVRRNGGELRDRTRGVYELPGHGYSSLVHEGEKRPFPRVEALSRDSWR
                     VTLDVAVEEYYQNEPVKKALVRYPLQVVRYAIDAEKNPWGLAIDCTDAAPQRLVAEEK
                     P"
     gene            2712..3479
                     /locus_tag="ACHLAM_004395"
     CDS             2712..3479
                     /locus_tag="ACHLAM_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO7855837.1"
                     /translation="MKPVLFTLLFSFPAFATEVVQWENIPLPVTLHVGQERIVDVGKP
                     VRIGYPATLEGKVRLQSAGGKVFLLANTAFPATRIQLRDTGNGELILLDIHATQGTST
                     LEPMQISYTPQTPATAKTSVPVTASTEPLPVLLVRYAAQNLYAPLRTVEALPGVTPAP
                     VRLAKRITTLLPQQPITATPLASWQVNATTVTAIRLQNHSGQRITLDPRELQGQFTAA
                     AFQHDWLGPRGLAEDTTVAYLVTEGPVSRALLPEPKP"
     gene            3476..4771
                     /locus_tag="ACHLAM_004396"
     CDS             3476..4771
                     /locus_tag="ACHLAM_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO7855838.1"
                     /translation="MMPRQSLLLKILLPLIVLVTVWLLLRPASKNTQETSLSRTVAAL
                     TPQELRDLGIDGDTPADTVATLVGQMKQYRRELQAIQTNNESQQAREKRLNAQQATLA
                     SQLREDMRRQQDQMRDELVKSQRGLLDKLDRLMAKRPDDIPPGLGLTPGELPADVQPD
                     IRWLEPLDAPPPVDEKLLRVPLKAADKPPPKPDVKVYTLPQNATLTGSVAMTALIGRV
                     PLNGTVSDPYPFKLLIGEDNLTANGVELPDVAGAVVSGIASGDWTLSCVRGQITSMTF
                     VFHDGIIRTVPQGGKSGNKQQEIGWLSDNQGLPCIPGERKSNAKEYLTSQFLLAGSGA
                     AAQAFANGESTTVVEDGSITSAVTGNNGQYVLGQAIGGGLKETADWMKQHYGQMFDAV
                     YVPPGHKVAVHLTEALSIDYEPEGRQVKYQQASTPGGLE"
     gene            4768..5127
                     /locus_tag="ACHLAM_004397"
     CDS             4768..5127
                     /locus_tag="ACHLAM_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO7855839.1"
                     /translation="MKFTILILLLLLAGCSTSQEELMPVDPNASMLTLWQQQSGGNAA
                     LLDARAQLRRPPDGPVAQPEADTIRSQFPRLPNPDMVLYVFQHLNSNTPVPGYSTVFP
                     FYTRPQYALPGERLTPP"
     gene            5124..7766
                     /locus_tag="ACHLAM_004398"
     CDS             5124..7766
                     /locus_tag="ACHLAM_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO7855840.1"
                     /translation="MNERRGLTEQALNTSYRKGPSFVDWLPWVEFLPESQTLLLEDGR
                     SVGAVFELYPVGTEGRSTAFLEQVRDTVTDALQDAFDEHERAPWVVQFFCQDEDDSEA
                     WLASLASYAAPEIRDSAFTREWLTQMANHMVNLTRDEGLFLDNTVTRTRWRATWRRTR
                     MVVYRWLPGLSPDEPSPEETLNNICERLTAGLKAAGISAIRQNEQDIHRWLVRWFNPA
                     PPAEDKKAFWQQLDLPETEPLPVLADFAERLLASEPRSDAANGVWWLDNRPHKVVVVD
                     RLRKAPPPGLLTGETARGDGINALVDLLPGGTIMNLTVVVYPQDKLEQHISRLADRAI
                     GDNTDSEYTRRDCSEVRSFLKDGHKLYRSTLAFYLSAPDTDILFRQQRQLQSLLLNAG
                     LVPVQEQHEVAPLSHWLRWLPMCFDPLRDPRGLYTRLNFVQHIAGLLPLYGRETGTGH
                     PGISYFNRGGTPLCFDPLNKLDRAQNGHLLLLGPTGAGKSATLNAKIAQLMALHRPRL
                     FIIEAGNSFGLMADYAAAHGLTVNKISLRPGSGVTLPLFADAWRLVEQAVTDAEPPED
                     EESDGDDQRDVLGEMEITARLMITGGEAKEDARLTRPDRAMIRQAIIAAAHTCYEEKR
                     QTLTQDIRDALFALSRDNSLPEARRQRAYEMAESINLFCAGFEGELFNREGQMWPEAD
                     ITLVDLATFAREGYEAQLAISYISLINHINNLGERAQHLARPIVNITDEAHIITVNPL
                     LARFLTKGSKMWRKLGIWLWLATQNLADFPDEAKKLLNMIEWWELLVMPPEEIEQVCR
                     FKALTEEQSQLLRSAVKSPGQYTEGVVLSPRIEALFRVVPPALYLALAMTEKHEKADR
                     MRIMKETGCSEVEAAITLSSVISQ"
     gene            7815..>8202
                     /locus_tag="ACHLAM_004399"
     CDS             7815..>8202
                     /locus_tag="ACHLAM_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855841.1"
                     /translation="MTAIACWFNREESDSIWAVSDSRITQQNSRLTDHCPKLFSIPVS
                     VIRSSDVYRIHPQKIFEFGFGFAGSTMIGINVKEMLAVSLARLHEIANNNPEQQISTE
                     AFPSLNEIASLAKNIAEQYMSDIGQSF"
BASE COUNT         1828 a         2421 c         2307 g         1646 t
ORIGIN      
        1 acaaaaatct cgcttaaata atacagtgcg atacgaaaaa ctatccagaa agaaaggatt
       61 caacttctgg ttatccttac aacttataat acctggagta gtacaaatat taccaatgat
      121 gtttctgttt aaccaactta atttcagtga gggtactagt gactggtatg agatgagcat
      181 actcattgcc ataactgtag ttattgttac aattggcatt ctgcctggta tcatacatat
      241 taatgaacgg caaaacggca ataaaatgac aggaattatt gttgttttaa tatttatacc
      301 tgtcgcaacc gctgcactta gtgtttggta cagacctatt cccaacatga tagctaatat
      361 gacaatgaat ctttcaggga ttagtgacca gagaacccat gaatactata ttgaaagagc
      421 aactcaccca gcaggtatgt ttaatggtaa aatatggaat actcgatact ataaaaacat
      481 ccctgataga ttcttcatta ctggagttaa cacatttact ttagggaata taaaactcat
      541 ctgcccaaca gcaatagtca aagcaagaat agaaagtttg aaattcactg taaatgacat
      601 tgacgagtac gaacaaaaag ggaaaaaact aaaaaagaca gcgatgaaat gtattccctt
      661 tgataaaaat gatatacata cctgggactc gccattatct gaaccaattt attacgagaa
      721 aatcaaacag accattgaca actctatgct aaaaattctt catgtagtga aatgattttt
      781 tagctgcttc acctctgaag ctctcctact ttgctaatca ttgagtataa ggagagcttc
      841 aaatgcagtc ccagacagca ctattactgt taataatacc tttcccggta ctggtaatcg
      901 cctccccgga atccacccag ctcgccctcg cgctgaagca actcgacggc gttaaaacct
      961 cgctggagca cgcccggacc agcgccagca acgatccgca ggcgcgctac ttcttcgact
     1021 acttacaggc gcagcaggac atctctgtcg ttgaacaagg cattcgccgc tatcttgccc
     1081 cggctagagc acagccgcgt gctattcagc ccctgagcgc caactaccag caggagaatc
     1141 ccgatgactg actcgcaact cgccgccttt caggccgcca gcctgagcaa accggagacc
     1201 gtcagcctgc tggtgctcgg cctgtttacc agcgtcctgc tgctgtggtg tgcctggacg
     1261 ctaatggtcg cctggcaggg cgtctgccgc cagcgtctga gctggcagag ctttggcgcg
     1321 gtggcggccc attcactgtt actggtgcta gtcagcttct ggctggtact gagctgagga
     1381 ggcatcatgc gctggtttct gttactgctg ttcgccacat ttaacgccct cgccgacctg
     1441 ccgacgatgg aagatccgtc acgcggtcag ggcaagggca ttatggagac gctgcaaaac
     1501 tacggttttg acatcgtggt gctgctggcg ctggccatct gtgccgtcgg ttttctggta
     1561 gtggcgaaca actgtattgc tacctattcg gagatccaga gcggacgcaa gcagtggaaa
     1621 gatctgggct cgatggcagg cgtcggcagc attcttctgg tggtcatcat ctggctgctc
     1681 aaccaggctt cggacgtcct gtgatatggc aaccatcccc tttctccccg accgcctcaa
     1741 ccgcgagccc gtggtctggc gcggcctcac caccagcgaa ctgtttctgg cgctggctct
     1801 cggtcttggc ggcggctgtg cgttcggcat tctgctggcc ctgataaccc actactggcc
     1861 gttgatcccc ggttcggcac ttgctggcgc ggcattactg attcagggtg gtgggcggat
     1921 tctggcccgc gcaaaacgcg gtaagcccga cacctggctg ccgcaggcgc tcctcgcctg
     1981 gctggagcgt catcatcttc gcggcgcaca actgatccag cacagcgccg tgtgggtggt
     2041 gcgcaggagt ccacgatgag ccgctttcgc catgcgctgg ctgagcgcga taaccatatc
     2101 ctgacgctgc gggcggtgat tgttctgctg tgcctgtgtc tggcgggcgt cggctacggc
     2161 tggtacagcg cgccgcaaaa gctcaccctc tggttcccgc cggatctgcg cagcggcagc
     2221 acccgcccgt ggtgggaagt gcctcccgcc agcgtctacg ccttcacctt ttatatcttc
     2281 cagcagctta accgctggcc ggataacggc gaggaggact attcccgtaa tcttcgttcg
     2341 ctgcgtgcct acctgacgcc cgcctgttac acccggctgg agcacgattt tcaggtgcgt
     2401 cgcaacggcg gtgaactgcg cgaccgcacg cgcggcgtct atgaactgcc cggccacggc
     2461 tacagcagcc tggtgcatga gggcgagaag cgccccttcc cgcgcgtgga ggcgctgagc
     2521 cgggattcat ggcgggtaac gctggatgtg gcggtggaag agtactacca gaacgagccg
     2581 gtgaaaaaag cgctggtgcg ctacccgttg caggtggtgc gctacgccat cgatgcggag
     2641 aaaaatccgt ggggactggc gatcgactgt accgatgccg cgccgcagcg tctggtggcg
     2701 gaggagaaac catgaagcca gtcctcttta ctttattgtt cagtttcccc gccttcgcca
     2761 cggaagtggt gcagtgggaa aacatcccgc tgcccgtcac gctgcatgtc gggcaggagc
     2821 gaattgttga cgtgggtaaa ccggtgcgca ttggctatcc cgccacgctg gaaggcaaag
     2881 tgcgtctgca aagcgcaggc ggcaaggtct tcctgctggc gaacacggcg ttcccggcca
     2941 cccgcatcca gttacgtgat accggcaacg gtgagctgat cctgctggat attcatgcca
     3001 cccagggcac ttcgacactt gaaccaatgc aaattagcta tacgccgcag acgcctgcca
     3061 cagcaaaaac atccgtcccg gtgacagcat caacagaacc tctgcccgtc ttgctggtgc
     3121 gctacgccgc gcaaaatctg tatgcgccgc tgcgcaccgt cgaagccctg cccggcgtca
     3181 cacctgcgcc cgtccggctg gcgaaacgca tcaccacttt actgccgcag cagccgatta
     3241 ccgccacccc gctggcgtca tggcaggtca acgccaccac cgtcaccgcc atccgcctgc
     3301 aaaatcacag cggacagcgc atcacgctcg atccgcgcga gcttcagggg cagttcaccg
     3361 ctgccgcgtt ccagcatgac tggctcggcc cgcgtggtct ggcagaagat accaccgtgg
     3421 cgtacctggt gacggaaggt ccggtttccc gcgccctgct gccggagccc aaaccatgat
     3481 gccgcgccag agcctgctgc tgaaaatcct gctgccgctc atcgtactgg taacggtatg
     3541 gctgctgctg cgcccggcca gcaaaaacac gcaggagact tccctgtccc gcaccgtggc
     3601 ggccttaacg ccgcaggaac tgcgcgatct gggcatcgac ggcgacacgc ccgccgacac
     3661 cgtcgccacg ctggtcggcc agatgaaaca gtaccgccgc gaacttcagg caatccagac
     3721 aaacaacgaa agccagcagg cgcgcgaaaa acggcttaac gcacagcagg ccacgctggc
     3781 ctctcagctt cgggaagaca tgcgccgcca gcaggaccag atgcgcgatg aactggtgaa
     3841 aagccagcgc gggctgctgg ataagctcga ccgtctgatg gccaagcgcc cggacgatat
     3901 cccgcccggt ctggggctga cgcccggcga actacctgcc gacgttcaac ccgatatccg
     3961 ctggctggag ccgctggatg cgcctccacc tgtggatgaa aagctgctgc gcgtccctct
     4021 gaaagcggcc gacaaaccac cgccgaaacc tgacgtgaag gtctacaccc tgccgcagaa
     4081 cgccacgctg accggatcgg tggcgatgac ggcgctgatt ggccgcgtgc cgcttaacgg
     4141 caccgtcagc gatccctacc cgtttaaact tctgattggc gaggacaacc tcaccgccaa
     4201 tggcgttgag ctgccggacg tggcgggcgc ggtggtctcc ggcatcgcct cgggagactg
     4261 gacgctctcc tgtgtgcgcg ggcaaatcac ctcgatgacc tttgtgtttc acgacggcat
     4321 catccgcacc gtcccgcagg gcggcaaatc cggcaacaaa cagcaggaga tcggctggct
     4381 ttcggataac caggggctgc catgtattcc cggcgagcgt aaaagcaatg cgaaggagta
     4441 cctgacctcg cagttcctgc tggcgggttc cggtgcggcg gcgcaggcct tcgccaatgg
     4501 cgaaagcacc acagtggtcg aggacggcag catcaccagc gcagtgacgg gcaacaacgg
     4561 tcagtacgtg ctcggccagg ctattggcgg cgggctgaaa gagaccgccg actggatgaa
     4621 acagcactac ggccagatgt tcgacgccgt gtacgtgccg ccgggccaca aggtggcggt
     4681 gcatctcacc gaagcgctgt ctatcgacta cgagccggaa ggccgtcagg tgaaatacca
     4741 gcaggccagc acaccgggag gtctggaatg aaatttacca tccttatact tctgttgtta
     4801 ctggccggat gcagcacctc gcaggaggaa ctgatgcctg tcgaccccaa tgccagcatg
     4861 ctgacgctgt ggcagcagca aagcggcggc aacgcggctc tgctggatgc ccgcgcccag
     4921 ctacgccgcc cgccagatgg tccggtggcg cagcctgagg cagacactat ccgcagccag
     4981 tttccgcgcc tgcccaaccc ggatatggtg ctttacgtct tccagcacct caacagcaac
     5041 acgccggtac ccggctacag caccgttttc cccttctata cccgtccgca gtatgcgttg
     5101 ccgggcgaac gcctgacgcc gccatgaatg aacggcgcgg cctgactgaa caggcgctga
     5161 ataccagcta ccgcaagggc ccgtcatttg tcgactggct gccgtgggtg gagtttttgc
     5221 cggagtcgca gacgctgctg ctggaagatg gccgctcggt aggtgccgta tttgagctat
     5281 atcccgttgg caccgaaggc cgctcaacgg cgtttctgga acaggtccgc gataccgtca
     5341 ccgatgcctt acaggatgcg tttgatgagc atgagcgcgc accgtgggtg gtacagtttt
     5401 tctgtcagga tgaggacgac agcgaggcgt ggctggcatc acttgcctct tacgccgcac
     5461 cggaaatccg cgactccgcc tttacccgcg agtggctcac acagatggca aaccatatgg
     5521 tgaatctcac ccgcgacgag ggactgtttc tcgataacac cgtcacccgt acccgctggc
     5581 gggcgacgtg gcggcgcacg cggatggtgg tgtaccgatg gcttcctgga ctttcaccgg
     5641 atgaaccgtc gccggaagag acgctgaata atatctgcga acgccttacc gccgggctaa
     5701 aagcggcggg tatcagcgct atccgccaga atgagcagga cattcaccgc tggctggtgc
     5761 gctggtttaa tcccgcgccg cctgctgagg acaaaaaagc cttctggcag cagcttgacc
     5821 tgccggaaac tgaaccgctg ccggtcctgg ccgatttcgc cgaacgcctg ctggcgagtg
     5881 aaccccgttc ggatgcggca aacggcgtct ggtggctgga taaccgcccg cataaagtgg
     5941 tggtggttga ccgcctgcgc aaagcgccgc cgcccggcct gctgaccggg gaaaccgcgc
     6001 gcggagacgg cattaacgcc ctggtcgacc tgctgcctgg cggaaccatc atgaacctga
     6061 ccgtggtggt gtatccgcag gacaagctgg agcaacacat cagccgcctg gccgaccggg
     6121 cgattggcga caataccgat tcggaataca cccgccgcga ctgcagcgag gtgcgaagct
     6181 tcctgaagga cggccataaa ctctaccgca gcacgctggc gttttatctc agcgcgccgg
     6241 acaccgatat cctgttccgc cagcagcgtc agctacagag cctgctgctc aacgccgggc
     6301 tggttccggt gcaggagcag cacgaagtcg cccccttaag ccactggctg cgctggctgc
     6361 cgatgtgctt cgatcccctg cgcgacccgc gcgggctgta tacccggctc aactttgtgc
     6421 agcatatcgc cgggctgctg ccgctgtacg gacgggaaac cggcaccggg catccgggga
     6481 tcagctactt caaccggggc ggcacgccgc tgtgcttcga tccgctgaac aagctggatc
     6541 gtgcccagaa cggacacctt ttacttcttg ggccaaccgg agcgggcaaa tccgccaccc
     6601 tcaacgcgaa aatcgcgcaa ctgatggcgc tacatcgtcc gcgcctgttt atcatcgagg
     6661 ccggaaactc atttggcctg atggccgact acgccgccgc ccacggcctg acggtgaata
     6721 aaatcagcct gcgccccggt tctggcgtca ccctgcccct gttcgccgat gcgtggcgac
     6781 tggtggaaca ggcagttacc gacgccgaac cgccagagga cgaagagagc gacggcgatg
     6841 accagcgcga cgtgctgggt gaaatggaga tcaccgcccg gttgatgatt accggtggtg
     6901 aagcgaaaga ggatgcgcgc ctcacccggc cggatcgggc gatgatccgc caggcgatta
     6961 tcgctgccgc gcacacctgc tacgaggaaa aacgacagac gctgacccag gatatccgtg
     7021 acgcactgtt cgccctttcc cgcgacaaca gtctgccgga agcccgccgc cagagggcgt
     7081 atgaaatggc ggaatccatc aacctgttct gcgccgggtt tgaaggcgaa ctgtttaacc
     7141 gtgaaggcca gatgtggccg gaggcggata tcaccctagt ggatctcgcc acctttgccc
     7201 gcgaaggcta tgaagcgcag cttgccatca gctatatctc gctgattaac catatcaata
     7261 acctcggcga gcgcgcccag catctggcga ggcctatcgt caatatcacc gatgaggcgc
     7321 atatcatcac tgttaatccc ttattggcgc gctttctcac caaaggctcg aagatgtggc
     7381 gcaagctcgg tatctggctg tggctcgcca cccagaacct ggcggatttt cctgatgagg
     7441 cgaagaagct gctcaatatg atcgaatggt gggaactact ggtgatgccg ccggaagaga
     7501 tcgagcaggt ctgccggttt aaagcgctta cggaagaaca gagccaactg ctgcgctcag
     7561 cggtaaaatc accggggcag tataccgagg gcgtggtgct ttcaccgcgt atagaggcac
     7621 tgtttcgggt ggtgccgcca gcgctctatc tggcgctggc gatgacagag aaacatgaaa
     7681 aggccgatag gatgcggatt atgaaggaga cagggtgtag tgaagtggag gcggcgataa
     7741 ccctaagctc ggtaatctcg caataaaaat atgctaagct attcgcatta atgttaaata
     7801 actggaaaaa ccttatgacc gcaattgcat gttggtttaa tagagaggaa agcgatagta
     7861 tttgggcagt cagcgactca agaatcactc agcaaaatag cagactaact gaccattgtc
     7921 cgaaattatt ctcaatcccc gtatcagtta ttcgctcttc ggacgtttat cgaattcacc
     7981 cgcaaaaaat attcgaattt ggcttcggat ttgcaggaag tacaatgatt ggaattaatg
     8041 tcaaagaaat gcttgcagta tcattagcaa gacttcatga gattgcgaac aataatccag
     8101 aacaacaaat atcgaccgaa gcattcccaa gtcttaatga aatagcttca ttagcaaaaa
     8161 atattgccga gcaatatatg tctgatatcg gccaatcctt tc
//
LOCUS       DAZGAP010000199         8050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGAP010000199 DAZGAP010000000
VERSION     DAZGAP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.200"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            73..2160
                     /gene="iha"
                     /locus_tag="ACHLAM_004400"
     CDS             73..2160
                     /gene="iha"
                     /locus_tag="ACHLAM_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO7855842.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSSLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            complement(2378..2528)
                     /locus_tag="ACHLAM_004401"
                     /pseudo
     CDS             complement(2378..2528)
                     /locus_tag="ACHLAM_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3022..3264
                     /locus_tag="ACHLAM_004402"
     CDS             3022..3264
                     /locus_tag="ACHLAM_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855843.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            3555..3923
                     /locus_tag="ACHLAM_004403"
     CDS             3555..3923
                     /locus_tag="ACHLAM_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855844.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            3927..4139
                     /locus_tag="ACHLAM_004404"
     CDS             3927..4139
                     /locus_tag="ACHLAM_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855845.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            4136..4408
                     /locus_tag="ACHLAM_004405"
     CDS             4136..4408
                     /locus_tag="ACHLAM_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFO7855846.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
     gene            complement(4717..5548)
                     /locus_tag="ACHLAM_004406"
                     /pseudo
     CDS             complement(4717..5548)
                     /locus_tag="ACHLAM_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5606..5794
                     /locus_tag="ACHLAM_004407"
                     /pseudo
     CDS             5606..5794
                     /locus_tag="ACHLAM_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5863..7089)
                     /locus_tag="ACHLAM_004408"
     CDS             complement(5863..7089)
                     /locus_tag="ACHLAM_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7855847.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(7162..>8050)
                     /gene="iolG2"
                     /locus_tag="ACHLAM_004409"
     CDS             complement(7162..>8050)
                     /gene="iolG2"
                     /locus_tag="ACHLAM_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFO7855848.1"
                     /translation="SSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAG
                     KHIFCEKPLSMREDEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQ
                     PQMIKITSRDPELLPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDP
                     TLKNIDDVDTLVVMLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAV
                     KVYNSQNCIMKNPLPDFALRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIA
                     AKQSLKSGLPVKIKTDIQL"
BASE COUNT         2233 a         1975 c         1861 g         1981 t
ORIGIN      
        1 atataaatta ttctcattat tgtttgtatt tgtgtattgt cttgccgatt aacatgatcg
       61 gagattagta atatgcgaat aaccactctg gcttccgttg tcattccctg tctcgggttt
      121 tcagccagca gcatggcagc agaggatgtg atgattgtct cggcatccgg ctatgagaaa
      181 aaactgacca acgcagccgc cagtgtttcc gtaatcagtc aggaggaatt gcagtccagc
      241 cagtaccacg atctggcgga ggcactgaga gctgtggagg gtgtggatgt tgaaagtggt
      301 acgggtaaaa ccggtgggct ggaaatcagc atccgtggaa tgcccgccag ttacacgctg
      361 atactgattg atggtgtccg tcagggcggg agcggggacg tgacgcctaa cggtttttct
      421 gccatgaata cagggttcat gcctcctctg gccgcgattg agcgtattga ggttatcagg
      481 gggccgatgt ccacactgta tggctctgat gcgatgggtg gtgtggtgaa tatcattacc
      541 agaaagaatg cagacaaatg gctctcttcc gtcaacgccg ggctgaatct gcaggagagt
      601 aacaaatggg gcaacagcag ccagtttaat ttctggagca gtggccctct tgtggatgat
      661 tctgtcagtc tgcagatccg cggcagcaca cagcagcgtc agggctcatc ggtcacatca
      721 ctgagcgaca catcagccac gcgtattcct tatcccacgg agtcacagaa ttataatctt
      781 ggtgcgcgta ttgactggaa ggctacggag caggatgtgc tctggtttga tatggatacc
      841 acccgacagc gttacgataa ccaggatggt caactgggta gcctgacagg gggatatgac
      901 catactctgc gctatgagcg aaacaaaatt tcggctggct atgatcatac tttcaccttc
      961 ggtacctgga aatcgtatct gaactggaac gagacggaaa ataaaggtcg tgagcttgta
     1021 cgtagtgttc tgaagagcga taaatggggg cttgccggta aaccgcggga gcttaaggaa
     1081 tctaacctta tcctgaattc attgctgctt acccctctgg gagaatctca tctgcttact
     1141 gtggggggag agtatcagag ttcgtccatg aaagatgggg ttgtccttgc cagcacaggt
     1201 gaaactttcc gacagaaaag ctggtcagta tttgctgagg atgagtggca tctcacggat
     1261 gtacttgcgc tgactgcagg cagtcgctat gaacatcatg atcaattcgg gggacacttc
     1321 agtccgcgtg catatctggt ctgggacgtg accgatgcct ggacgctgaa aggcggtgtg
     1381 accacgggat ataaagcccc caccatggga cagttacata aggggatcag cggtgtatcc
     1441 gggcagggac gtactaatct actcggtaac cccgaactga agccggaaga gagtgtaagt
     1501 tatgaggcgg gggtatatta cgataacctc tccggtctga atgccaatat aacaggcttt
     1561 ataacagact tctccaacaa gattgtctct tattccattg atgataacac caatagctat
     1621 acaaacagcg gaaaggcccg cttgcatggt gtggaattcg ccggcacatt gccgctgtgg
     1681 tcagaggatg tcactctgtc gttgaattac acctggaccc aaagtgaaca acgtgatggt
     1741 gataacaaag gtgcaccgct gagttatacc cctgaacaca tggtgaatgc gaaactgaac
     1801 tggcagatca ccgaagaggt ggcatcatgg ctgggtgccc gttatcgcgg gaaaacacca
     1861 cgtttcaccc ggaattattc gtcactgagc gctgtacaga agaaagtgta tgatgagaaa
     1921 ggagaatacc tgaaagcctg gacggtggtg gatgcaggtc tgtcgtggaa gatgacggat
     1981 gccctgacgc tgaatgctgc ggtgaataac ctgctcaaca aggattacag tgacgtgagc
     2041 ctgtacagtg ccggtaagag ttcgctgtat gccggtgatt acttccagac gggatcatca
     2101 acaacaggat atgtgatacc tgagcgaaat tactggatgt cgctgaacta tcagttctga
     2161 taataacaaa acgctatcac tgacggtaga atacgttgcc actgcaactc ctggcggaac
     2221 agtggcaacg tattaggtta agtgcatttc cgatccgcta atgagatttc gttaccaaca
     2281 actaatatcg tcacaggaaa tgcacggatt atttttaact tatcatttac atacttgtcc
     2341 agagtgtaag cgcaccgtga aggacgtggg gtaaaaatta gtttacagag agagtgacgt
     2401 tccagggcaa caactctttc atgcggttgg caggccaggt gttgattaca ctgatcacgt
     2461 ggcgtagcca cgtttccggc tcgattccgt taagttttgc agctaccgat caggctgtac
     2521 atcactgccg cactatcgct cgtcatctca aagtccggtc tcgtcagcag gaaggtatca
     2581 ttctctcccg ccatttttcc gggggcccgg tcagataagt ccctttgtct atcgctgact
     2641 cctggctcat aacccggtta gcagaatgca gggtcaccac ctcgccacga ccgaatccgg
     2701 ataagtcaat tgcaccttct caatcgccat tttgtcacaa ttaagtttga taaagcaggt
     2761 cacctctgca ctgaatctgc tccagtgcct cattggtctc cgtccccccc tttaaactgc
     2821 cggataagac tgctgcacag cgccttcccc ggaatcaaat cactttccct gtccgccaca
     2881 atccgcttca tgctacctcg tattttgttt cagagaccgg aatctgccag aaagtgacta
     2941 cgcgtgcaca tatcggtttt ctgggttaca tgcatcgtgt tattttctga aatgcaaaat
     3001 aataaaaaac aaggacacat aatgaaggat gaaatacaca gtaccagcat caacccggat
     3061 gttgttgagc agtttctggc ccttatgaac acacattgta aatcctcaga agaggcgctc
     3121 gccatgttga gtacgctttg ttacaacctg atggtgacat gtggcatcca ttccatggac
     3181 agccagatgc tcaatgggca tgggatatcc atgcaacttc aggggattga tattgccgaa
     3241 aggccaggta agaatattca ctaacctgaa cagggcctga aaacgctgtc ttttcaggcc
     3301 tctgcaggca aaaactgatg acaacaaaat ttctgcgttt gtaccccacc gatgaaaaag
     3361 acaaaaactc acaccgggct tctagccagt aaagataaaa cccgacgtgt cagcctctac
     3421 gaaacgccaa cggcatggtg tatcaggggg caggagtgct acagcaaaag tacaggacgc
     3481 cgctgtggca gccacgacag tctgagcaga ctgcgcctag acagcattaa gcctgttgaa
     3541 tgagtggtgg caccatgaaa gataacaaca caaccgccac cccagaagcc tggaaaatgc
     3601 gttttctggc caacgggaca tccggcacat ggttttacac tgacgacatt gcagtcactg
     3661 acgcgtggct tcgcgcgcac ccggatgatg tggaggttat ccctctctgc cggataccac
     3721 cccgtcagca ttcccatgaa caacatattg aagaactccg cgcagccctg gcccggatgc
     3781 tgcatatgca tcatatgatg ctggaaacca cgcattacag ggaaaacatc accgatgccc
     3841 gttgtatccg tgaaatgcat gaagctccat tacaggcgct caatattctg gccagatacc
     3901 cgcataaaaa ggacgaggtg tgacgcatgt ggctactgat tttctggctt tcatctccct
     3961 ttaatgtctc ttccggtgat gcccggcagc ccacactggc ggtgcacagc cagacatttc
     4021 ataccgaacg ggcctgtcgc gatgcattgc gcgccattaa aaaggagaat gttggcgaat
     4081 tttctctgcg gggcgtttgt gtttctgacg gaaacacgaa ggaggcagag agctcatgac
     4141 ggatattact gacagacata cacttcgccg catgtcctgg tcggaactta ccacggcagc
     4201 gcaggaagca gaattaaacc gtgattacga gcgtgccaga atagtgtggt cattcgcctt
     4261 acatgttgca acaaccacga ttaacaaaaa tctggccata gctcatattc gacgctgcga
     4321 tactttgttg cataaatcaa aaacagtacc cggaaacaac ccgagaggcc ggtcagtgtg
     4381 cctgagacct caacatcccc ggagatgact gacattccgg actgacgcag ttgctgcata
     4441 tctgtggcaa tacgttgtat ccagccagac aggctatccc gcgtagtgac aattaacccg
     4501 ctttcctgca ccagcttcag ccgggccgtc ggggatttta ccagccggaa atatcagtaa
     4561 aacaacatat cattatgcca tggtgtacgc ggccagtgta tgcttactta aggaatgagg
     4621 gataaattgg atattattac ttcactttgc ttttttatga gattcatcac ataagtgggg
     4681 ttttactgtt aaataatctg aatttatttg atatccgtaa agcagatgtc tcagttcact
     4741 gtttccaact tttgttattc ctctggggcg aatgctgttt ccgctggaat attcacctgg
     4801 caccagtccc agaaaagcca tgagctgttc aggattggaa aatcgcgaaa aatcacccag
     4861 ttcagcaacc agggttattg caattagctg accaacacct ttaagagcct gcaactggca
     4921 gaccagattg cacagagatc actcaggtaa caggcgagat atttcctgat cgagttgaag
     4981 tatgcgatct tcaatctgct ccagattctg acaataatgt tgaaagcaat ctgttgagat
     5041 agcagaccaa atgattgatt aactaaccag gttcgatgtt tgccggttca gtgcttcctg
     5101 gcataacgtt tgtctgttcg tgtcacgttt tgcagcggca cgggctctga ttaaatcccg
     5161 catagcttca tgagtcagct cagggagcca gactggggga acctcaccat ccctcagtaa
     5221 cctggcgaga gaaattacgt cccgatggtc atttttgatc ctgtctgccg gtgatttggg
     5281 tatacgggaa gggggcaata accaggcatt ttatattcat cactgtgagt tgatgatata
     5341 gcccaaaccc acagggacca ccttcatagc aggaaagaat gtttccgtaa ggtccctgca
     5401 atttctgaca taatcgatgt acagccaagg cttcgttgtt aatcgtacca taataacgaa
     5461 cttcgccaag ccattcaggt gtagcaatcg ccaaggtttc tttatgaaca tccagcccga
     5521 cgtaagctgc aatattttcc gaatccatga tgacctctgg agtatcccac caatgctata
     5581 gtgagtatat aaaggtcata tcatctacgt gttcttcggc aacgatcaca gtggagagcg
     5641 tggagcgcta ctgtacgggc tgattggcac ctgccgactg aacagtatcg atccggaagc
     5701 gtatctgcgc catattctga gcgtactgcc ggaatggcct tccaaccgtg ttgacgaact
     5761 gctgccatgg aacgtagttc tcaccgataa ataagcgtca atacggcatt cgattaatgc
     5821 acttacagct aaatggagtt tttgaacaat atttgacgtt atttatttct caccaggaac
     5881 tttaagaaac tgaaccagga tgccactaaa taaatagagt cctgccatag tatacataac
     5941 cacgcctgta ccaaaacctg tcagaatgga taccaacgca ggtccgacaa aatttcccag
     6001 gccagaaccg aaattcaccg ctgagtttgc tgcccccttc ttatccggtg ccatcatcgg
     6061 taccagcggc gtcatcggca caaatcccgc cagccccgct ccccacaggc acattgccag
     6121 cataaaagca acgtaattat gcccccacac tatcggcacc cagtagacaa tcagcgtggc
     6181 aaacccggtc agaagactgc caaaaatctg gatagtacgg cgaaaaccta ttttatcccc
     6241 catccagccg aaaataatgt tgaaaatctg attagaaata aaggttatac cccagatact
     6301 cgcccactcc tcaagggtaa actgtttatc cagcaaaaac agcggcaaca ccactacaaa
     6361 accataggtg ccgagtccat tgattgtttt aacaatcact gccagaccaa ttcggggtcg
     6421 ttgatacata atggaaatac ctgccgacaa ctctttaaag actgacgtag ttcttggctt
     6481 cagaatgaac gcattgcgct caaagaccag catgaaaatc cccccaagca gtgcaacccc
     6541 aatcccggcc cacataacat taatatgccc aaggacagga attaacgaag cagctactac
     6601 cggaccaatg atggtaaaac ctaagttata tactacccaa aacagcgacg ttccaattcc
     6661 ctgttgatga ttatcgagtt gaatggtcag tcggacaaga aaggaatagc agaccaacgg
     6721 ataacctact ccccgcagca tatagctaat gagaattgta ctaaaatcat ggctgggaat
     6781 cgcaaaatta agaaacgcga tagaagcgat caggtaaacg gagaaaccag caagcataac
     6841 ctttctgggg ccaaacatat ccacacagac accggataac caggaagtta cggcaaccac
     6901 aattccgtag gcggcaaata tctgtccgat ctgagactga tcaaatccct gagtattcag
     6961 ccaggcagat agccaactat tttcaataca gccgcccaac ataaataaaa atgttcccag
     7021 gtagctccag ataaactgac ctggctttat ataaaatgtc tgaggcaaca ttgcctgcat
     7081 atctttcatt ttataaccct cagtacggat aatctctcgc tcgtaatagt gcgagagacc
     7141 cttcgctatt tacaaatgca actaaagttg aatatcagtt tttatcttca ctggcagtcc
     7201 actttttaat gattgttttg cagcaatggc aatacgctgc gccaataatg catcttcacc
     7261 agtacagaca atgggtgtat gattcagaac agaatcaata aagtgtaata tttccgttct
     7321 ataagcttca cgatagcgta gcgcaaaatc aggcaaagga tttttcatga tacagttctg
     7381 actgttatat accttcaccg ccgattcact gacattgtcg gcatacgcca tccctgctga
     7441 accaaagacc tcaacccgtt ggtcgtagcc ataaaccgcc cgacgactat tatcaattag
     7501 cgcataacca ccgtttctga aggtcagcat caccacgaga gtatctacgt catcaatatt
     7561 tttcagagta ggatcaatca atgtattgcc tttaacataa acctcgctaa cttcatcctg
     7621 cataataaag cgagccatat caaagtcatg catagtaaaa tcaaatatga gtccacctat
     7681 cctcttgatc aaatcgtgcg gcaacaactc tggatcacgt gaggtgatct ttatcatctg
     7741 aggctgacca atcttgcctc tgcgcaccag ttcatacacc tcccgaaact ggggatcaaa
     7801 acgacgatta aaagcaatct gcagtttgac tcctttctct ttaaccgccc ttagcaccgc
     7861 aagggaagct ttctcatctt ctctcatgct gagaggtttt tcgcagaaaa tatgtttacc
     7921 cgcctggact gctgccgtaa cgatctcttc gtgcatatct gtcgaggtga aaataaacac
     7981 cgcctcaata tcatcacgct gtaacatttc ctgataatct ttatatcctg aggttatatt
     8041 taccgaactc
//
LOCUS       DAZGAP010000200         7980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGAP010000200 DAZGAP010000000
VERSION     DAZGAP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.201"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(92..379)
                     /gene="yebO"
                     /locus_tag="ACHLAM_004410"
     CDS             complement(92..379)
                     /gene="yebO"
                     /locus_tag="ACHLAM_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO7855849.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(454..597)
                     /gene="mgrB"
                     /locus_tag="ACHLAM_004411"
     CDS             complement(454..597)
                     /gene="mgrB"
                     /locus_tag="ACHLAM_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO7855850.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            756..995
                     /gene="yobH"
                     /locus_tag="ACHLAM_004412"
     CDS             756..995
                     /gene="yobH"
                     /locus_tag="ACHLAM_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO7855851.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="ACHLAM_004413"
     CDS             complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="ACHLAM_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO7855852.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2107..3480
                     /gene="yebQ"
                     /locus_tag="ACHLAM_004414"
     CDS             2107..3480
                     /gene="yebQ"
                     /locus_tag="ACHLAM_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7855853.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3526..4407)
                     /gene="htpX"
                     /locus_tag="ACHLAM_004415"
     CDS             complement(3526..4407)
                     /gene="htpX"
                     /locus_tag="ACHLAM_004415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO7855854.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4599..6647)
                     /gene="prc"
                     /locus_tag="ACHLAM_004416"
     CDS             complement(4599..6647)
                     /gene="prc"
                     /locus_tag="ACHLAM_004416"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO7855855.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6667..7365)
                     /gene="proQ"
                     /locus_tag="ACHLAM_004417"
     CDS             complement(6667..7365)
                     /gene="proQ"
                     /locus_tag="ACHLAM_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO7855856.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7462..7959)
                     /gene="msrC"
                     /locus_tag="ACHLAM_004418"
     CDS             complement(7462..7959)
                     /gene="msrC"
                     /locus_tag="ACHLAM_004418"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO7855857.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
BASE COUNT         1892 a         2104 c         1816 g         2168 t
ORIGIN      
        1 taatttgacg cagttgcacc atgacagtgc gtataatcag gatgaattga gtaacccttc
       61 aacgacgggg ttactcaaga ttgaggaaag attatcgttc ggcgaccaga cgaataatat
      121 cttcgtcttc aaccgatttt tgactctcaa cggtcttttt atctgctttc tctggttcgt
      181 ttgcttcgca caaacgacgt aacagagcat tttgacgttt ttgctgatcc agcaacgcct
      241 caagcagttc aatctgttcg ttagtccgtg aactggcacg attgataaaa aaccacaaga
      301 taagcccgat aaaaagaacc accaccgata caaccaaaga cgcaatgttc atcacgcctg
      361 aatttacaac ttcgttcatt tcaccacctc tgagtagagg cgctattcta ccactgctgg
      421 agaggaagaa aatctagtgc tgaaaaaatg atatcaccac gggataaact ggttaatggc
      481 acaaattccg ctgaaaaatt gtacatcctg atcgcacatc atgttgaata cctgcgccca
      541 aagcagcaag caagccaaca ccacgacaac cagaacgacc catcgaaact ttttcactcc
      601 aatctccgtt tcacctacct tatgtcatta aactagcatg cgttagttaa acagcgccta
      661 actatgtcga tattcgtgct ttttcagaat gagtcgcttg cttcagcgca tattgccgct
      721 acgattaagc gaacataaaa agagaagagg ttgtaatgcg attcatcatt cgcacagtta
      781 tgctgatagc actggtatgg ataggattat tactcagcgg atatggcgtt ttgattggaa
      841 gcaaagagaa tgccgcggga ttaggtttgc aatgtaccta tctgaccgcc agaggcacca
      901 gcacggtaca atacctgcac actaaaagtg gttttctggg gataacagat tgcccgttgt
      961 tacgtaaaag caatattgtg gtcgataatg gttaagggta ataaataacc agattctcac
     1021 tcatgagtaa aatattctta tcgcaagtgc gtttaatata acgccacctt caactttatt
     1081 tttctcatgc taaataaaga acctgtctgt ttgatattca gacaggttct tactctcatc
     1141 agaacggata gtcgtgataa cccatttggg cagaaatttt gcgcgccgcg gtatgcaaca
     1201 ttgcgacata ttcctgtaaa cgctcttcag agaaacgtaa cgtcgggaag gagatgctca
     1261 aaccggcaat gaccacgcca aagcgatcaa ataccggaac cgcaatgcat cgcagccctt
     1321 cttcctgctc ttcgttatct tcgccatacc cctgctcgcg cacctggtcc agaacgggta
     1381 ataacgcttc tgtactggtg atggtccgct cggtactgcg tttatactcc acgccctcaa
     1441 gaatttgctt cacttcatcg cgatcgcgcc atgccagcag taccttacca atcgcggtgc
     1501 tgtacagcgg attacgacgc ccaatccgtg aatacatgcg caaattgtac atagagtcaa
     1561 ttttgtgaat gtaaacaata ctgtcttcgt ccagtgcgcc gaggtggata gtttctttgg
     1621 tcaggcggga gatctcacgc atctggatat ctgcgctacg aattaaatcg acgttttgta
     1681 acgcgcgagc gcccagttca aacaatttca gggtcagcga atatttctcc gactcccctt
     1741 cctgcgccac ataacctaag gttttcatgg tctgtaaaaa gcgataaacg gtgctttttg
     1801 acatcatgac gcgctgcgac aactcggtta tccctatttc gcgctcttca cccagcgcct
     1861 gcaaaatgcc aaaaactttt agcacggaag atacagaatc aggctgttta tccagatctg
     1921 cgttagccat ttatcacctc attgcgagtg ttttataaaa atcagaactg ttttttatta
     1981 taatttcgca ccagggtggt cgcaatccat cttttgccgg ttagttacaa ttctgcgaca
     2041 tccaccgtga atatcagtgc tagaatcata cccctgttga ttattcacca aagatataaa
     2101 attcctatgc caaaagttca ggccgacggc ctgccattgc cccagcgata cggtgcgata
     2161 ttaaccattg tgattggtat ttcgatggct gtccttgacg gcgcaatcgc caacgtcgcc
     2221 ttgccaacaa tcgccacgga ccttcatgcc acgccagcca gttccatctg ggtagtgaac
     2281 gcctatcaaa tcgccattgt catctccctg ctctcgtttt cgtttctggg cgatatgttt
     2341 ggctatcgac gtatttataa atgcggtctg gtcgtttttc tgttgtcttc actattctgc
     2401 gccctttctg attcgctgca aatgctcacc cttgcgcgtg tcatacaagg tttcggcggt
     2461 gcagcgttga tgagcgttaa taccgcactt atccgcctga tctatccaca acgttttctg
     2521 ggtagaggga tgggcataaa ctcgtttatt gttgccgttt cttctgctgc cgggccgaca
     2581 attgctgcag caatcctctc catcgcatcc tggaaatggt tatttttaat caacgtaccg
     2641 ttgggtatta tcgccctgct tctggcgatg cgttttctgc cacccaatgg ttctcgcgcc
     2701 agtaaacccc gtttcgacct gcccagcgcc gtgatgaacg cgttaacctt cggcctgctt
     2761 attactgcat tgagtggttt cgctcagggg caatcgctga cattgattgg tgcggaactg
     2821 gtggtaatgg ttgtcgttgg tattttcttt attcgccgcc agctttctct tcccgtaccg
     2881 ctgctaccgg tggatttact gcgtatcccg ctgttttcac tttctatttg cacatctgtt
     2941 tgctctttct gcgcacaaat gctggcaatg gtttcccttc ccttttacct gcaaaccgtg
     3001 ctcgggcgta gtgaagtcga aacaggttta cttctgacac cgtggccgtt agcaacaatg
     3061 gtgatggctc cactggcagg ctatttgatt gaacgcgtac atgcaggatt gctgggtgct
     3121 ttagggttat tcatcatggc tgcggggctt ttttccctag ttctgctgcc agcgtcacct
     3181 gcggatatca atattatctg gccgatgatc ttatgtggtg ctggatttgg cttgttccag
     3241 tcacccaata accacaccat tattacctcc gctcctcgcg aacgtagcgg tggagccagt
     3301 ggcatgttag ggacggctcg tcttctgggt cagagtagcg gcgcggctct ggtggcgctg
     3361 atgctaaatc agtttggtga taatggtacg cacgtctcgc tgatagctgc ggctattctg
     3421 gcggtgattg cagcctgtgt cagtggttta cgtatcactc agccacgatc cagggcataa
     3481 aaaaagcgcg tcgatcagga cgcgcttttt agtatttact tcatattact tcaggtattc
     3541 acccgtacgc agagcttcaa tacgtttatc cagtggcggg tgagtcatga acaactcact
     3601 gagcgatttc gacttcccgt taatgcagaa agccatcatg ctggttgctt cttgcggttc
     3661 atagctggtt ttcaggcgct gcaatgcagc aatcattttc tcgcgaccaa ccagttttgc
     3721 tgaacccgca tcggcgtgga attcacgatg acgcgagaac cacatggtga taatgctcgc
     3781 cagaatacca aacaccagtt ccagaaccgt tgcaaccgca aagtagatca gcgggttgcc
     3841 gttgctctct tcaccttcat cacgatttcc gcccataaaa cccgcggcaa gctgcgccag
     3901 aatacgggaa ataaagataa cgaaggtgtt caccacgccc tgaatcagcg tcatggtgac
     3961 catatcacca ttggcgatgt ggctgatttc gtgagcaatt accgcctcgg cttcatccgg
     4021 gctcatgttc tgcagcaaac cggtgctgac agcaaccaga gaggcatcac ggcgcgcacc
     4081 ggttgcaaaa gcattgatgt ccggcgcatg gtagatagcc acttgcggca tagcgatccc
     4141 cgcctgacga gcctgggttg ctaccgtatt gaccagccaa cgttcccttt cgttacgcgg
     4201 ttgctcgatc acttccccgc caacagatcg taatgccatc catttggaca tcagaagcga
     4261 aacgaaggaa ccaccaaaac cgaacagcaa ggccatgatc atcagcccct gaacgctgct
     4321 cgactgtatc cctgtcaggc tcagtaccag cccgaaaacg accattacgg ccaggttcgt
     4381 tagcaggaag agcgcgattc gcatcataat tttcttttta cctcagttta acaaaacgca
     4441 atatgcgata cccacatcgt atgggttacg cgactatttt caagtctgga tagtgcgtaa
     4501 gtcaccagaa agacacaact ttacattttg tagcatctga tttacggcat cttgctgctg
     4561 ttaaaaaatc aggcacaatt tcttgtgcct gattgatatt acttgacggg agcgggttgt
     4621 tccgcgggtc tggctttttc aagcttcgcc agatcgagtg cgatattcac cgtctcatcc
     4681 agataaggat ccggctcctg gtaatctttc ggtagatcat ccagtttctt caactccggt
     4741 ttaccttcgc gtttaaagcg ttcgttcaaa cgcgccagac gcgtcgcatc atcttcatta
     4801 ttctctttct cacgcacagc gtaattcaga gaaacgatat tgcgcttgtc cttcatggcg
     4861 ttgaagcgcg cgatatcctt catgatgttc tggaactcag gatctttcgc gatacgcgca
     4921 ttatgttcct tcagcagctc cggtccaaag gccgttaaat cgcctgattt cacataggtc
     4981 gcggcatcga tgctatccca cggcagcgcg ttatcttcga atttctcacc cgtttccgtt
     5041 tcttcattac ccgtcggcat gatgatgtct ggcgttacac ctttacgttg cgtactgccg
     5101 ccgttaacgc gatagaattt ctggatcgtg tactgtacag atcccagcgc tggccattca
     5161 ggacgtaaca tctgatcgta gatacggttc aatgaacggt attgctgaac ggtgccttta
     5221 ccaaacgtcg gttcacccac aaccagcgca cgaccgtaat cctgcattgc cgcggcaaag
     5281 atttctgaag ccgaagcact gaaacggtca accagtacca ccagcgggcc tttatagaag
     5341 acctgtccgt cggtatcgct gtcttcacga actttaccgt tgttatcacg gacctgaaca
     5401 atcgggccgg atggaataaa cagaccggag agcgataccg cttcggtcag cgccccacca
     5461 ccattgctac gcaggtcgat gatgacgctg ctgacattct gtttttccag tttctgcagt
     5521 tgcactttga catcgtctgt caaacccaca tagaagcccg gaatatccag cacgccgact
     5581 ttctctttac cgacggtctt caccgacatt ttaaccgcgc ggtcttcgag gcgaatacgt
     5641 tcacgggtca acgttacagt acgggtcttg gtccctttac cagcaggtaa aatttccaga
     5701 cgaactttac tgcccttcgg ccctttaatt aaggcaacca catcatcaag acgccagcca
     5761 atcacgtcaa ccatcggctt gcctgtttga ccaacaccga caattttgtc accaacgctg
     5821 atagctttac tcttcgctgc cgggcctcct gccaccatcg aattgataac ggtgtagtca
     5881 tcatccattt gcagcactgc gccaatacct tccagcgaca aactcatttc agtgttgaac
     5941 tgttcggtat tacgcgggga aagatagttg gtatgcgggt cgatttcacg cgcaaacgcc
     6001 gtcattgcca gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg aatggcaaat
     6061 ttgtagcggc gagtcagggt ttcacgaatt tctttatccg tttttcctgc cagcttcagg
     6121 cttaactcgt cgaatttgac tttactgtcc cacagcgcgt tcaactcagc ctcgtttttc
     6181 ggccagggcg ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc
     6241 ttttccagta ccgacaaagc gtactggtaa cgttcaaaac gacgcttttg cgccagattg
     6301 tagagatcgt agaaaacgtc gagtttgcct gaacgcagtt catcgcctaa ctcggttttc
     6361 tttttcgcga actgttcaac atcgcttgcc aacagcacgt tgtggctgta atcgagcaga
     6421 ttcaggtagc ggtcaaagat tttggccgaa aatgtctgat cgaggtcgaa ctggcgataa
     6481 tgagaacggg tgaagcgcga cgttacgcgc tcacttaccg tcgcatgctg cgtctcttcc
     6541 tttaataccg gaatttgatc agcacgcgtg atatcttcta cagcgaaggt ctggcctgct
     6601 attgcaagca ggccagctaa cgcggtaagc ctaaaaaaca tgttcatgcc tggcccggcc
     6661 tccgtttcag aacaccaggt gttctgcgcg cacaatcaaa gacatacccg aattcagctg
     6721 gacgcggacg ccgtctttgg tgatttctaa tacggtggca tccatcgcgt tttgacccgc
     6781 tttcaccttc agggcttgtc cgacagtcag agctgaaatg tcagaaaccg gggtgtgctg
     6841 ttcttcgcga ggtgctttta ctgtttttgg cgctttctct accggctttt gcgcacgagg
     6901 tttacgttca gcgccttctt tgcggcgtgg cgtggtcgga cgtggcttgc gttcgcggcg
     6961 cggtgcgtct tctttctcac cagcagctgc ggcagcttcg cgttttttcg cttgctgttc
     7021 agcacgctgt gcctgaacac gcgctttcgc ttcttcaagc tgcttgcgag catgctctac
     7081 atgttgctcg tccagctcac cgcatgggtt gccgtcaaga tcgacacgcg ttgcgccggg
     7141 tttaacaccg taaagataac gccagctcga agtgtagaga cgtaaagcgg atcgcaattg
     7201 cgttttgctc aggttcattt ccccggcaac acgatcgacc aaatcctgaa aaataccgat
     7261 tttcagcgga cgcgcttcac cttccgcact gaaacagtgg ggaaaacgtt cggccagaaa
     7321 cgcgattact tctttactgc tattcaactt aggttgattt tccatgaaat ttcctgatta
     7381 caacggacgt agccaacaag cgcaggcatg aacaggcgtc attataatga cgctatcagt
     7441 aaatgctacg ttatccgttg attatcctgc gacgctcgca aagaattttt tgtaatccgt
     7501 cgttgcaagc actttttcaa gctgtgccac aagctgacgt aagccttgct cgtcctcgtc
     7561 tgtaaagcga ccgaagacgg tactatcgat gtcgagaaca ccaataatct gatttttcac
     7621 caccagcggc agaacaattt cagaattact cgccgcatca caggcaatat gcccgtcaaa
     7681 cacatgaaca tcctcgatac gctgcacttg attgcgggca accgcagtgc cgcacacgcc
     7741 gcgcccgacg ggtatccgga cacaggcaat tttgccctga aatggtccga gtaccagtgt
     7801 atcgtcctca agcaaataaa aacctgccca gtttacgtca gtgagacgct catataacaa
     7861 cgcactggtg ttcgcaagcg ttgccagaaa actggtttct cctgccatca gcgcgttaaa
     7921 gtcgcgattt aaatccgcgt aaaattctat tttgttcatt atgtaatcac ttggttgtct
//
LOCUS       DAZGAP010000201         7914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGAP010000201 DAZGAP010000000
VERSION     DAZGAP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.202"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /gene="yiiE"
                     /locus_tag="ACHLAM_004419"
     CDS             <1..216
                     /gene="yiiE"
                     /locus_tag="ACHLAM_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO7855858.1"
                     /translation="AMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQQ
                     FPVRKAKGGRQRGETVVVDDQCKEHKE"
     gene            complement(431..1360)
                     /gene="fdhE"
                     /locus_tag="ACHLAM_004420"
     CDS             complement(431..1360)
                     /gene="fdhE"
                     /locus_tag="ACHLAM_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO7855859.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1357..1992)
                     /gene="fdoI"
                     /locus_tag="ACHLAM_004421"
     CDS             complement(1357..1992)
                     /gene="fdoI"
                     /locus_tag="ACHLAM_004421"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7855860.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1989..2891)
                     /gene="fdoH"
                     /locus_tag="ACHLAM_004422"
     CDS             complement(1989..2891)
                     /gene="fdoH"
                     /locus_tag="ACHLAM_004422"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO7855861.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2904..5954)
                     /gene="fdnG"
                     /locus_tag="ACHLAM_004423"
     CDS             complement(2904..5954)
                     /gene="fdnG"
                     /locus_tag="ACHLAM_004423"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5367..5369),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7855862.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKVYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6148..6981
                     /gene="fdhD"
                     /locus_tag="ACHLAM_004424"
     CDS             6148..6981
                     /gene="fdhD"
                     /locus_tag="ACHLAM_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO7855863.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7070..7760
                     /locus_tag="ACHLAM_004425"
                     /pseudo
     CDS             7070..7760
                     /locus_tag="ACHLAM_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         1865 a         2016 c         2088 g         1945 t
ORIGIN      
        1 gcgatgaata cggtttttct tcatttatca gaagaggcaa ttaaacggct gaacaagctt
       61 aggggctggc gtaaggtttc acgatctgca attttacgcg aagcggttga gcaatatctg
      121 gagcggcagc aatttccggt gcgtaaagca aagggtggca ggcaaagggg tgagacagta
      181 gttgttgatg atcagtgtaa ggagcataag gaatgacaaa aaatttatta ttcttgaata
      241 taaaaaccag atgcccttat tctggtatta atacaagact gttttacttg aacttataat
      301 aactgtaact gttacatcat atctggaaaa cgcctcgcaa aatacgagag attttgcggg
      361 aatagcaggt gtcgtcacac cctatgagct gtaatccggg cgaccagcgc cacccggaaa
      421 acgtcagaga ttactcccct tcacccggga acaggaacgg gttgatggaa ctgcgggcgt
      481 agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct gccacggctt
      541 caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccacag tcatcgcagc
      601 tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt ttgccgctct
      661 gctcgcagtt gctgcatttt acgcgcacca cgtgccattc ggtttcacac aggttgcaat
      721 gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc ggcatagagc
      781 cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg ccggggatca
      841 gattagccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc gctttatcgc
      901 tgctgacgga cgagaaatca gaggcaaaca gtgcgctggc catatcttcc agctcctgag
      961 tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt tcaggtttca
     1021 gctcagcaat cagcgccatc agcagctttt gccagtgctt atcacgcggc agaacgtgaa
     1081 tatccagcgg gggcttgcct tgtgcgctgg cttctttaat gcgcgtggtc agatccatct
     1141 ccagcggatg gtcgtacagc accacttcct gggcgtgggc gataagcgca gcaaagcgca
     1201 ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg cggcggttgt
     1261 ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta cgtttctcgc
     1321 tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct tttccgttgt
     1381 cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc tggtaaccca
     1441 tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga tatgcaccat
     1501 gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga atcggatcac
     1561 cgggattgag aaagcaggtg caaaataagg acgccagata atcacgccgc tcaccagcag
     1621 cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt tataacgccc
     1681 ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc aaaagatatc
     1741 atcccgattg attaggttgt gatgccagta acggaaaaac atgatgatga acgaggcaaa
     1801 cataaccacg ccgacaaacg ggtgcagaat gcgcgccagc tgcggtgtgc ccatgatttg
     1861 catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg ccaggatgaa
     1921 gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca cgatggtgtc
     1981 acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc ttcctcatcc
     2041 gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt agccgcaaag
     2101 ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc gctgatttcc
     2161 gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag cacgtacata
     2221 acatgtgtac caccgacgcc tgccgggtcg tacaaacccg cattgtcgta accacgggtt
     2281 ttcagctccg ccacgcgctc gctcgccagc gttttcatcg actctttcgt accgaagtga
     2341 atcgcgcccg ttgggcaggt cttcacgcag gccggttctt gcccaaccac cacgcggtca
     2401 acgcacaacg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg aatgtcgaac
     2461 ggacagcccg caatgcaata accgcagccg atgcactgct cggactggaa gtcgacgata
     2521 ccgttggcat actgaataat tgccccttcc gccgggcacg ctttcaggca gcctggatcg
     2581 gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt ctgctccact
     2641 tccgagaatc gcatcaccgt ccacgatttg gcacttaaat cattggggtt gtcgtacacc
     2701 ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc cacctgacag
     2761 gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc ctggaagtcc
     2821 cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat atcttgcgat
     2881 tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa ggacttaaac
     2941 tccggcgtct gcgtgttcgc atcaccgacg aatggcgtca acgtattggc aataaagcct
     3001 tttttcgcaa caccttcata gccccagtga atcggaatac cgatggtatc gatatctttg
     3061 ccgtttgctt tcagcgtgcg aatacgtttg gtcactaccg ctttggcttt gatatagcca
     3121 cggttggagg agactttcac agtatcgccc tgggcaatgc caagtttatt cgccagcgac
     3181 tccccgattt ccacaaactg ctctggttgc aaaatcgcat tcaacagcgc gtgtttggtc
     3241 cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa cttatcggct
     3301 ttacccaatg cttcggcgtc gtctttaaag atacgcgcag ccgggttcga gataacgttt
     3361 ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc cgggaacgga
     3421 ccttccgcca tcttatcgag ggcaaacaga cgtcccatgc cttcctgctg catgataaac
     3481 ggccctacgc cgctgcccgg aggcgctgcg ctgtagtccg gaatatccca gccggtccac
     3541 ttagtgccgt cccatttcag caactgacgc ttcggatccc atgggttacc ctgcggatct
     3601 gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc ccagcccagc
     3661 gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt gccttccggc
     3721 gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc gcgcagttga
     3781 gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc cggatcggta
     3841 atatcggcca gcgccttacc gttgctctcc atcgccactt cttctgaaga tggctcatgc
     3901 ggaatggcgt agttccaggt catgttcagc acctggtccg ggtttgcgcc accctgttct
     3961 gcatacatct tgcgcaagcg caggaagata ccggagagga tctcgccatc agtcagcgca
     4021 atccctgggg cgtccgcacc tttccagtgc cactgtaacc agcgaccgga gttaacgatt
     4081 gaaccgttct cttctgcgaa gcaagtcgat ggcagacgga acacttcggt ctggatcttc
     4141 gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt ttcagtgttc
     4201 agcgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac tttgtttttg
     4261 ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac cttgccttct
     4321 ttcatcatct cgaagtattg caggacgtcg tagactttat cccacttcgg caaccaatca
     4381 aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat catagagacg
     4441 aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg ttttggcgtg
     4501 ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc tcggcagcgt catgtaaccc
     4561 ggcaggctct gcgacagcag ccccaggtcc gtcagcccct gaatattgga gtgaccgcgc
     4621 agggcgttaa cgccgccgcc tgccatcccc atattgccga gcagcaactg gatcatcgcc
     4681 atcgtacgaa tgttttgcgc accaatggag tgttgcgtcc agccgagcgc gtaaaggaac
     4741 gaggcagttt tatcgtgagc actggtctca gcgatgtatt cgcagacttt caggaatgcg
     4801 tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga aacgtgctgt
     4861 ttcagcaagt tccacacgca acgtggatgt tgcagggtag tatcgcgttt ggcgaagccg
     4921 ttttcgtcca gttcgtaagt ccaggaggat ttatcgtact tacgtttttc cgcgtcatag
     4981 ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct ggcgttggta
     5041 taggcttcgg tgtattcgcg gttgaatttt tcattgttca gcaggtacag caatacgcct
     5101 gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc taccgccgcc
     5161 gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cattgtgaat tttggcttcc
     5221 atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat caccacgacg
     5281 aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc aaatgttgga
     5341 gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac cgcgagcata
     5401 cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga cgcggaagca
     5461 cacagcatcc cggtggagag ccagcggtta acggtcacgc cttcggcgtt ttgcgcaatg
     5521 tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc ctcttcccaa
     5581 ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt cagacggctt
     5641 tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc gcggttgacc
     5701 gggtgatccg ggtcaccttc gatatggaag atagatgctt tggcgttttt tgctccgtca
     5761 ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt attacgggtt
     5821 tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct gggtgcaaaa
     5881 cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa gaactgcctt
     5941 ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt tacattcgct
     6001 tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc aaaggtccag
     6061 aattggctca atttccctcc atccaggaag ggtttgtaac agaataccat aatgttggtg
     6121 tgtgtgttct tatctggtta agagaaagtg aaaaaaacac agcaaaaaga aatcgaaaat
     6181 gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcatgatga tttacaacac
     6241 ccacggttag atgaggtggc ggaagaagtt cccgttgcgc tggtctacaa cggtatttcg
     6301 catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg tttttcgctt
     6361 tccgagggga taatcgaaag tccgcgcgat atcttcggca tggatgtcgt tccttcctgt
     6421 aatggtcttg aagttcaaat tgagctttcc agccgccgct ttatggggtt gaaggagcgt
     6481 cgccgggcac tggcgggacg tacgggatgt ggcgtgtgcg gcgtggagca acttaatgac
     6541 atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa caaactggat
     6601 gatgcattac gtcatctcaa tgattttcag ccagtggggc aactgaccgg ttgtactcac
     6661 gccgccgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga cgtgggtcgc
     6721 catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag ctggcagcaa
     6781 ggtgcggtac tggtttccag ccgtgccagt tatgaaatgg tgcaaaagtc ggcgatgtgc
     6841 ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacac ttgctgtaga agtggctgaa
     6901 cgctgtaatt tgacgctggt gggcttttgt aaaccgggtc gggcaacggt ttatacccat
     6961 ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa ttgcaggact
     7021 tattcatttc gtgaatttta ttattttatt tataaacaaa acgcgtgtag tgtcgttaac
     7081 gcctgcgttg caacctattg atggcgtggc ggtgagttat attgatgctg ccgtagcgtt
     7141 gggtaatacc attaacgaaa tggataaata ttacatccag gaaaattata aagacgatgc
     7201 gtttgcgaaa gggaaaacgc tgcaccagac attcttaaaa aatctggaag cctttgaacc
     7261 tgtagcagaa tcttatcatg cggcgattca ggaaattaat gacaagcgcc agcttacgga
     7321 attgaaaaat attgaagaaa gagaagggaa aacattccac tactactctc tggcagtcat
     7381 gatttcagcg aaacaaatta ataacctgat atcgcaagat aagtttgatg cagaagcggc
     7441 aatgaagaaa gtgtctgaac tggaaacgct ggtggcgcag gccaaagaag cggataaaag
     7501 cggcatgaat ttctcgttta ttaattcggc aggccagtat caactcgagg ctaaaaaata
     7561 cgttcgccgc atcagagata aagtcccgta ctctgactgg gataaagagc aacttcagga
     7621 cgcaaactca agctggatgg ccgaagactc atttcccgag agcattatgc gagtacaacg
     7681 aaatggtcga tgaaatattt cagtcaattg taatcgcgga gcgggtttgt gatgagcgcg
     7741 gatatgtcac gaagtcgtga caatataaag cagagtgaag gataaatttt gtgaataaag
     7801 tatcattatt taatgatttt gtgaatgttt aaatgtctca gataatttgt ttaagcgtta
     7861 tttatatttg tggcttatga gtcagcgtga atcatatcta atatttttta cgat
//
LOCUS       DAZGAP010000202         7836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGAP010000202 DAZGAP010000000
VERSION     DAZGAP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.203"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1882)
                     /gene="prpE"
                     /locus_tag="ACHLAM_004426"
     CDS             complement(<1..1882)
                     /gene="prpE"
                     /locus_tag="ACHLAM_004426"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO7855864.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AME"
     gene            complement(1922..3373)
                     /gene="prpD"
                     /locus_tag="ACHLAM_004427"
     CDS             complement(1922..3373)
                     /gene="prpD"
                     /locus_tag="ACHLAM_004427"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO7855865.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(3407..4576)
                     /gene="prpC"
                     /locus_tag="ACHLAM_004428"
     CDS             complement(3407..4576)
                     /gene="prpC"
                     /locus_tag="ACHLAM_004428"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO7855866.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(4736..5626)
                     /gene="prpB"
                     /locus_tag="ACHLAM_004429"
     CDS             complement(4736..5626)
                     /gene="prpB"
                     /locus_tag="ACHLAM_004429"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO7855867.1"
                     /translation="MYLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            5865..7451
                     /gene="prpR"
                     /locus_tag="ACHLAM_004430"
     CDS             5865..7451
                     /gene="prpR"
                     /locus_tag="ACHLAM_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO7855868.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGHEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQESETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(7552..7827)
                     /gene="yahO"
                     /locus_tag="ACHLAM_004431"
     CDS             complement(7552..7827)
                     /gene="yahO"
                     /locus_tag="ACHLAM_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO7855869.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
BASE COUNT         1716 a         2191 c         2118 g         1811 t
ORIGIN      
        1 cttccatcgc ctggcggatc tgatccaacg acgccggatc atcaatggtc gtcagatccc
       61 caggatcgcg tccttcgcaa atcgcctgga tcgtgcggcg cagcattttt ccggatcgcg
      121 ttttcggcaa ttgcgagaca aaccagacgt gcgccgggcg gccaaagttg ccaatctggc
      181 tgtccaccag cgccataatc gccttctctt gcgagtgcgc cacctcgcgg tcttccagac
      241 tgtcgctctc tttcggaatg acaaacgcca ccgccacctg ccctttcagc gcatctttca
      301 ccccaaccac cgccacttcg gcaacgcccg gatgactgga gatactctct tcaatctcgc
      361 gcgtccccag ccgatgcccg gcaacgttaa tcacatcgtc agtgcgcccg agaataaagt
      421 gataaccgtc agcatcgcgg atgccccagt caaaggtggc atacaccgga cgggaaaaca
      481 gcgaccagta cgtcttcaca aagcggtcgt catcgcccca gatggtctga atacaccccg
      541 gcggcagcgg cccttccacc accagcatcc ccttctcgtt gacgccgcac ggttcgccgg
      601 tgacttcatt gagcaactgc acgttatagc catacatcgg cacgccgggg cttcccagac
      661 gcgtcggcct gtcgtccaga ccgcgagcaa tcgccataat cggccagccg gattcggtct
      721 gccagtaatt gtcgatcacc ggcacatcca gcgtattgct cacccaactg gcggttggct
      781 cgtccagcgg ctcccccgcc agatagagca cttccagcga cgagagatcg tgtttgcgaa
      841 tttcagcggt agggaatttt ttcagcacgc gaatggcggt cggcgctgag aacatgcggc
      901 taacctgata tttctcgacg attttccacc acacgccgca gtccggccag gtcggtaatc
      961 cttcgtaaac gatagtcgcc atccccgcca gcagcggcgc gtagacgata tacgaatgcc
     1021 ccaccaccca gccgatatcc gaagcacaaa agaacacacc gcccgctttg ccgccaaaaa
     1081 tggtgtccat cgaggtcgcc agcgccaccg catatccgcc aacgtcacgt tgcacgcctt
     1141 taggtttgcc ggtcgtgccg gaggtgtaga gaatgcagga ggtttcgttg gattccagcc
     1201 acgccaccgg tacccgcgcg ccgatgtgtt gatggcgcaa cgacgcgaaa tcgacatccc
     1261 gcccgctgac gcgtgccatt ttcgccagcc cgcgatccac cagtaaaacg tgacgcggct
     1321 gatgctgtgc ctgacttatc gcatcgtcga gcaatttttt atagggaatg attttaccgc
     1381 cgcgcgcccc ggcatcagcc gagacaatca gcaccggttt agcgtcatca attcgcgttg
     1441 ccacgctgtg cgaagcaaat cccccaaaca ccaccgagtg aatagcacca atgcgcgcgc
     1501 aggccagcag ggtaatatgc gcttcggcaa tcatcggcat atacaccagc acccgatcgc
     1561 cacgctgcac gcccagtgag cgcagcattg acgccaccgc attcacttcg tcatgtaact
     1621 ggcggaaggt aaaggtacgc tcttcctctg tttccgaaga gacggcaatc aatgccagcg
     1681 cctctggctg tttctccagc cagcgatcga tagcgttgtg acacaagttg gttcggcctt
     1741 cacaaaacca acgggcaaac ggcgggttgc tgtggtcgag cgtttgcgta aagggcgtct
     1801 gccagtcaat acgccgggcc tgctcggccc agaacttctc cggttcgtta atcgaacgct
     1861 gataaaattc gctaaaagac ataatgctct cctgttgaac ttacgcctta ccgccgttta
     1921 cttaaatgac gtacaggtcg agatactcat tgaccggcat ctgttccagg cgagttctgt
     1981 cgagagaaac ctccagaatg cgctgctgct ggcgagtcgg gaactggcgc gcgagattga
     2041 ttttgaattt atcgaccagc ttcggaatgc catcctgacg gcggcgagca tgaccaattg
     2101 ggtactccac caccacttct tcaaatcgtg tgccgtcggt gaactcaagg gttatggcat
     2161 tggcgatggc gcgtttttcc gggtcgtggt agtcagcggt aaacgccgga tcttcaaagc
     2221 aattgatctt ctcgcgcagg gcgtcgatgc gtttatcttg cgcaacgttg tcctcgtaat
     2281 ctgccgccgt taagcgtccg aacagcagcg ggatcgccac catgtactga atgcagtggt
     2341 cgcggtctgc cgggttattg agcggccctt ttttgtcgat gatgcgaata caggcttcgt
     2401 gggtgcgaat ggtcactttt tcgatatctg ccgccgtttt gcctgctgcc tgcatctgtt
     2461 catagagcgt catcgccgct tcaactgccg tctgggagtg gaactccgcc gggaaggaga
     2521 ttttgaacag cacattttcc atgacgtagg aaccgtacgg acgctggaag cggaatgact
     2581 cacctttaaa ggagacgtcg tagaaacccc atactggcgc agtcagggct gacggataac
     2641 ccatttcgcc cgttttcgcc atcagtgcca gacgtaccgc gcgggaagtg gcatcgcccg
     2701 ccgcccagga tttacgcgtg ccggtgttcg gcgcatggcg ataggtgcgc agcgactgac
     2761 cgtccaccca cgccagcgaa acggcgttga gaatttcctc gcgggtcagg ccgagcattt
     2821 cggcgaccac ggcggtggaa gccactttca ctaacagaac gtggtcgagg ccgacgcggt
     2881 taaaggagtt ttccagcgcg atgcagccct gaatttcatg ggctttgatc attgcggtca
     2941 gcacctgctt catggtcaac ggcgctttgc cgctagcgac cgcgttgcgc gaaagccagt
     3001 ctgccgttgc cagaatgccg ccgaggttgt cggaaggatg gccccactcc gccgccagcc
     3061 aggtatcgtt gaaatcgagc caacggatca tcgcgccgat gttaaatgcc gcctggacgg
     3121 ggtcgagctg gaactgagtt ccggggacgc gcacgccgtt aggtacgacg gtgccgggga
     3181 caattggccc cagcagtttt ttacaggctg ggtattcgag agcttcaaga ccgcagccga
     3241 gcgtgtcgag caggcagtaa tgtgcggtgt cgtaggctac tttggagctg atttcgtagt
     3301 tcatcacgta atcgacgata tcaacgattt cacgatcaaa ttccgggcgg atgttgttga
     3361 tttgagctga cattgggtac gtttccttat tgttattcgt agaggtttac tggcgcttat
     3421 ccagcgcgac aaactggcgg tcttccggtc caacataatt ggcggaagga cggataattt
     3481 tgttgtcctg acgttgttcg ataatgtgcg ccgcccagcc agtgacgcgg gcgataacaa
     3541 acagtggtgt gaacatctcg gtgggaacac ccatcatgtt gtaggaaaca gcggagaacc
     3601 agtcgagatt ggggaacatc tttttgctct cccacatcac cgtttccagg cgatcggcga
     3661 tgttgtacat cttcagcgag ccgccttcct gcgagagctg cttcgccaca cgtttgatca
     3721 cctgatgacg cgggtcggcg atggtataaa ccggatgacc aaaaccaatg accacttctt
     3781 tgttttccac ccgcttgcgg atatcggctt cggcttcgtc cggcgtttcg tagcgttgct
     3841 ggatctccag cgacacttca ttcgccccgc cgtgcttcgg cccgcgcagt gcaccaatcg
     3901 cgccaataat cgcggaatac atatcagagc cggtgcccgc aatcacccgg ctggtaaagg
     3961 tggaggcgtt aaactcgtgt tcggcgtaca gcaccagcga aatatgcatc gccttttccc
     4021 agctttgcga cggcttttcg ccgtgcagca gatgcaggaa gtgaccgccg atggagtcgt
     4081 catcggtttc cggctgaatg cgttcgccgt tgtggctgta gtgataccag tagagaagaa
     4141 tcgagctaag tgacgccagc agtttgtcgg caatatcccg cgcgccagag acggtatgcc
     4201 cctctttttc tggcagcgtg cagccgagcg cggaaacgcc ggtgcgcata acatccatcg
     4261 ggtgtgacgc cgccggtaag gcttccagca cggtacgcac gttagccggt aaaccgcgca
     4321 gggctttcag tttcgttttg taggcggcga gttcgtcacg ggtcggcagt ttgccgtgga
     4381 tcagcagatg cgccacttct tcaaattcgc aatgttccgc cagatcaaga atgtcgtagc
     4441 cgcggtaatg caaatcattg ccgcttttac ccacggtgca gagcgccgta ttgcccgccg
     4501 gaacgccaga aagcgccacc gattttttcg gtttaatgac atgggtactg ttttgcagga
     4561 tcgttgtgtc gctcatatta tcctcgtcat tgttatcatt tgtagggtcg agattgttgt
     4621 cggatgcgac gctatgcgtc ttatccgacc tacagttcag ttcacctcgt aggcctgata
     4681 agtcgcgtca gcggcgcatc aggcatcggt tgtcgaatac aaccaacggg cgtttttatt
     4741 tcacctgacc acgggcaaac aggtcgtcga gcttctcttc gtactggtag tagttgatgc
     4801 tttcgtacag ctcgttgcgg gtctgcatgg tgtcgatgac gcttttctgc gtcccttcct
     4861 gacgcaggac gttgtagaca tgctcagcgg cgcggttcat ggcgcggaac gctgaaagtg
     4921 ggtacagcgc cattgcgaca tgggcgctgc gtaattcgtc ggtggtaaac agcggtgttg
     4981 cgccaaattc ggtgatgttg gcgaggatcg gcacctgcac cgcatcggca aactggcggt
     5041 acatggcgag ttcggtaatt gcctccggga acaacatctc ggcacccgct tcaacatagg
     5101 cctgcgcacg ctcaatcgcc gcatccagcc cctctaccgc cagcgcatcg gtgcgcgcca
     5161 tgatcacaaa atcaggatcg gttttcgcat ccaccgccgc gcggatccga tccaccatct
     5221 cttctttcga gacgatcgct ttattcggac gatgaccgca gcgtttcgca ccaacctgat
     5281 cttcaatatg caatcctgcc gcaccggctt taatcatcga tttcacggtg cgcgccacgt
     5341 taaaagcaga agaaccaaaa ccgatatccg catccaccag cagcggcagc gaacaaacgt
     5401 cggtgatacg gcgaatatct gtcagcacgt catcaagagt agaaataccg agatcgggca
     5461 gccccagcga ccctgccgcc acgccaccgc cggagagata aattgcctga tatccggcac
     5521 gctgcgccag cagcgcatgg ttagcgttga tggtgccaac aatctgcaat ggattttctt
     5581 tagacagtgc agcgcgaaac gctttacctg gagagtgtag atacatagcc catcctttgt
     5641 tatcaacttg ttatttgcgt tgataaacac aaagcaaggg gtgtgccaca attgactgaa
     5701 taaaattaaa tgtttaattt caatttgtta gcaatatagt catgaagtaa ttcgcaaata
     5761 tgcgtttcag ttaacgtttc aggcaatgtt tcaccgcgtt tcattgcaac aattatgaaa
     5821 caagactaaa cccaatattc ggtttcttaa ctttgcggtg cgctatggca catccaccac
     5881 ggctgaatga cgacaaaccg gtgatctgga cggtatctgt aacgcggctg ttcgagctgt
     5941 ttcgcgatat cagtctcgag tttgatcacc tggcgaatat cacccctatt cagcttggct
     6001 ttgaaaaagc ggtgacctac atccgcaaaa aactggcaaa cgaacgctgc gacgccatca
     6061 tcgctgctgg ctctaacggc gcatatctta aaagtcgcct gtcagtgccg gttattttga
     6121 ttaaaccgag cggctacgat gtgttacagg ctctggcaaa agccggaaag ctcacctctt
     6181 ctatcggcgt tgtcacttat caggaaacta ttccggcact ggtggcgttt caaaaaacct
     6241 ttaatttgcg cctcgatcaa cgtagctaca ttaccgaaga agacgcgcgc gggcagatta
     6301 acgagctgaa agctaacggc accgaagcgg tggtcggcgc ggggctgatt accgatctgg
     6361 cagaagaagc cggaatgacc ggaattttta tctattccgc cgccaccgtg cgccaggcat
     6421 tcagcgatgc gctggatatg acgcgcatgt cgttacgcca taacactcac gatgccaccc
     6481 gcaacgccct gcgtactcgt tacgtgctgg gcgatatgct cggtcaatca ccacagatgg
     6541 aacaggtgcg gcagactatt ttgctgtatg cccgctccag tgcggcggtg ttgattgagg
     6601 gggaaacggg gacgggcaaa gagctggcgg cccaggcgat tcatcgggaa tattttgccc
     6661 gccacgatgc gcgacagggc aaaaagtcgc atccgtttgt tgccgtcaac tgcggagcga
     6721 ttgccgaatc gctgctggaa gcggagctgt ttggccatga ggaaggggcg tttaccggct
     6781 cgcgacgcgg aggtcgcgcc gggctgttcg aaatcgccca cggtggcacg ctgtttcttg
     6841 acgagatagg cgaaatgccg ctgccgttgc agactcggct gctgcgggtg ctggaagaaa
     6901 aagaggtcac ccgcgtcggc gggcatcagc ctgttccggt ggatgtgcga gtcattagcg
     6961 ccactcactg caatctggaa gaagatatgc ggcaagggca gtttcgccgt gatctgtttt
     7021 atcggctgag tattttgcgt ctgcaattgc caccactgcg cgagcgggtg gcggatattc
     7081 tgccgctggc ggaaagtttt ttgaaagtgt ctctggcggc gctctccgcc ccgtttgctg
     7141 ccgcattacg ccagggatta caggaaagcg aaaccatgct ggtgcactac gactggccgg
     7201 gcaatattcg tgaactgcgc aatatgatgg aacgactggc gctgttttta agtgtggaat
     7261 cgacgccgga tttaacgccg caatttttgc agctgctact gccggaactg gcacgcgagt
     7321 cggcgaagac tcccattcca ggcttgctga cagcacaaca ggcactggag aaatttaatg
     7381 gcgataaaac agcagcggcg aattatttag gtatcagccg gacgacgttc tggcggcggc
     7441 tgaaaagctg aactgccgaa tgcagcgcga acgctttatc cggcataaat ttcaatagat
     7501 gatgatcacc gataaactta cgcatcgggg atgtttacat aggattcaga attacttctt
     7561 cttataaata tttgccgtgc cgtggatctt attgtcagtt tgaccagagg tcagtaccaa
     7621 cacatcagcc cctttttcat cggctttttt gatcaaatct tctttcgcat ctgcggtcga
     7681 catttcatta ctggttgaaa tatcacctat tttttcatat tgcgatgcaa ccttttcaaa
     7741 ttccgctttg gtcattaatt ctgcggcata gacattggta acggcaaacg ctaacgcacc
     7801 gactaacatt ttagagatta ttttcatatt acggct
//
LOCUS       DAZGAP010000203         7822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGAP010000203 DAZGAP010000000
VERSION     DAZGAP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.204"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..325
                     /locus_tag="ACHLAM_004432"
     CDS             71..325
                     /locus_tag="ACHLAM_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFO7855870.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            325..741
                     /locus_tag="ACHLAM_004433"
     CDS             325..741
                     /locus_tag="ACHLAM_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO7855871.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCSRLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            complement(1051..1254)
                     /locus_tag="ACHLAM_004434"
     CDS             complement(1051..1254)
                     /locus_tag="ACHLAM_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855872.1"
                     /translation="MPELPHFAPAPVPCPASALASQGGVELLTFGRLYRLTPGFGGFT
                     PLPYTAWSAAFHVAGAAYHGDDI"
     gene            1639..2001
                     /locus_tag="ACHLAM_004435"
                     /pseudo
     CDS             1639..2001
                     /locus_tag="ACHLAM_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            2005..2360
                     /locus_tag="ACHLAM_004436"
                     /pseudo
     CDS             2005..2360
                     /locus_tag="ACHLAM_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            2642..3781
                     /locus_tag="ACHLAM_004437"
     CDS             2642..3781
                     /locus_tag="ACHLAM_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFO7855873.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            3901..5169
                     /locus_tag="ACHLAM_004438"
     CDS             3901..5169
                     /locus_tag="ACHLAM_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO7855874.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            5177..6322
                     /locus_tag="ACHLAM_004439"
     CDS             5177..6322
                     /locus_tag="ACHLAM_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO7855875.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            6790..6936
                     /locus_tag="ACHLAM_004440"
     CDS             6790..6936
                     /locus_tag="ACHLAM_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855876.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQLRPGHYR
                     GMSM"
     gene            complement(7007..>7822)
                     /locus_tag="ACHLAM_004441"
     CDS             complement(7007..>7822)
                     /locus_tag="ACHLAM_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7855877.1"
                     /translation="FILEKTAHATLQINRGLLQQFGETIRVDFQHKNDIEEAFKYASI
                     NNMVPISISDSVGSMGGVAPVEFIVRMANRFDGYAYIDDAHGMSIYGKNGSGYVMSCL
                     DNKLDDRIIIAGSLSKAFGSHGGFIACNYPDTINFIKTYGTTYAFGGPPSLPGIAACI
                     AAADLHLDGTVSLRQNDLQQVIKYFDDKFNETEVINKGTTMPIRGLLIGNEEKAITIC
                     KKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENIDSLHKNFIECINEQG"
BASE COUNT         2009 a         1918 c         1906 g         1989 t
ORIGIN      
        1 atttcttgca ttgtactatt tcatgcccta caatgtagta acgtacctat atacatcaaa
       61 aggcaacagc atgaagatta caaccctatc cagtcgggaa cttaatcaag atgtaactcg
      121 agccaaacgc gccacgaatt atggtccagt atttattact gatcggggta aaaccgcaca
      181 tgtacttctt agtatcgaag aataccgaaa attgacgcaa caacgacgaa ctctcattga
      241 cattctggcg atgcccgatg ctgatgcagt tgaatttgaa ccgcctcgta ttacggttag
      301 tttaaaacct gcggagtttt tatagtgtat attcttgata ctaatgttat ctctgagcta
      361 cggaaggctc ggacaggacg tatagataat aatgttgcaa aatgggcatt gcatacagat
      421 gcggcagatt tctttatttc tgttatttcc ataatggaac ttgaacaggg aatattacaa
      481 gttgaacgtc gagatatgcg acaagggatt atgctacgtt cttggatgga tcagcatgtg
      541 ttaccagcgt tttctgataa gatacttcct gtagatactg cggttgcatt acgttgttct
      601 cgtttacatg ttcccgacag gcgcagtgaa cgagacgcat tgatcgcagc aacggcgctg
      661 gtacatggga tgacggttgt aaccagaaat gttggtgatt ttgaattaac cggagtatca
      721 attattaatc cctgggcttg atatcgcttc ccttcttcta ggggtccgct tggaaaacgg
      781 aaattttccc acgctaagag aattgagttg aacgagtggt tacatccaag ccagctaact
      841 cactgaacgt aaaggctgta cgaaatgttc cgaccgtccg gattcctgac agtcatttgg
      901 ctaaaaaata gtcttaacgt ggtttatttt ccgaattcca ggctgacccc ataaaagcat
      961 ccttcagttc agcgtcgatt cttaaactca tattaacctg tgccattcat cacctcgtca
     1021 ttaccacgta tatacacagt atataattat tcagatatcg tcaccatgat atgccgcgcc
     1081 agcaacatgg aaggcggcac tccatgctgt atatggcaga ggagtaaaac cgccgaatcc
     1141 cggcgtaagc cggtacagtc gtccgaatgt cagtagttct actcccccct gcgaggccag
     1201 tgctgacgcg ggacatggaa cgggtgctgg agcgaagtgg ggaagctccg gtatactggc
     1261 caaacctaca cgcctccttc cgtctccggg aagatgctgg cttcgtaccc tgcgtgcgtc
     1321 tgatctgtag ctggccgtgg cacggctacg gacagattct gctccgcaac agtatcggcg
     1381 tgcctgacct cctgaaagtc ccgtaacgca ttttctcacc tcccatgccc tgacgctatt
     1441 gagcgcgtta cagaacaatt taagtcattt aacgagcctc caacggctcg atcgaaataa
     1501 atcaaatcgt aatcgtcaac gatatatgag aggagacccc gctcgccgca aatcgatgac
     1561 cgaacgtagt gagcgaatcg acgaggaagc ggaagagaac ctgtacgaaa atggagcaat
     1621 tacgccccac cgtcaagaaa tgagctggct ctccacggcg cgccatcata gacagatgtg
     1681 gctctgcaac tgcgccatgc gatcggagac cgaccagtaa ttatctttaa tgcccggttc
     1741 gacacccgca ttctgaaaca gactgctgcc gcacatagcg atccggctga ctggctggaa
     1801 gaactgacag tatattgtgc gatggaactg gcagcaggat attatggggc caccaaccgc
     1861 tatggcacga tttctctggc ctgtgctgcc agcaggccgg actgacatgg gaagggctgg
     1921 cacactcagc tgatcgccga cacacggatg acgtcagggg tggtaaacgc tattactgca
     1981 tatcatcagg aattgctgca gcttatgtac tgcgataacc ctgaactcaa ctatgcaaac
     2041 ctgaaatccc tgcaaaaaca gctggatgaa atgtatacct gcatccgtaa taatcagatg
     2101 atgctcccaa actacggaaa aatgcgccgg tatggtgaac cggtttcaac aacgtttgta
     2161 gagtcgacaa tcaacgaagt gatcgccaga cgatgaccca aaagcaacag atgcagtggg
     2221 gtaaggaagg tacgcattat ttgttacaga ccagaaccgc tgtcctgaat aattaactgc
     2281 aggacaaatt cgccagttgg tacccgggtt gcacgataga cgctcagagt ggaggaaagt
     2341 tatccgccat ggcagcataa ctacttcccc agttttttca gctctccctt accaatatcc
     2401 tacgacgatc acatcgaaaa aaaaacacgg aacaggcgaa cagaaatata attaattatt
     2461 tgagtttaaa ggaaaaaaga ggatacaagc tttttgttgt atttttattt gtataaaccg
     2521 acaaaaaaga ggccatactt cccgctttct ttgtctgttt acacggaaca tttcaccact
     2581 ggatatgcta atagatcgga tatgagcaca tattgcgccc gcctttcatg cagagcataa
     2641 aatgaacaag attcacgcta attattacct gcaagattct accgcacgcc agatagttca
     2701 acgcacaatg gggattattc cgtactcggt aaatgtgatg aacgagcatg ggatgatcat
     2761 cgcttccggg gaagcctcac gtttacatca acgccatgaa ggtgcggtac tcgcattgac
     2821 cgaaaaccgg gtcgtcgaaa ttgattccgc gaccgccaat aagcttaaag gcgttaagcc
     2881 cggaatcaat ttaccgatta attttcgcga ccagctgatt ggtgtactgg gcattaccgg
     2941 cgagcctggt gaagtgaagg catatgcaca gttggtgaag atggcttcgg aattaatcat
     3001 tgaacaaatg gctctcctcg agcaaaaaca gtgggataag cgttaccgcg aagagctgat
     3061 taatcagctg attttacgcg atccgccacc ggactcactg aatgcggtgg tctcatggtt
     3121 gggaattaat ctccagcttc cgcgggtggt ggtgattatg gaactgcaac aacctgatcg
     3181 cgacgcactg cataatctga tggagtattt tgaatatcgc gcccgcgacc atttggtcac
     3241 gttttccgac tttaatgatc tgataatctt aaaacctatc tcgctaaaaa acggcgtctg
     3301 ggataaatcg ttggaattaa gcgatctgca gagacttaaa cattacgccc agtcctgtgg
     3361 tttttcccga ctgattgtcg gcggctattt ctctggagaa aacggcctgc atcgctcttt
     3421 tcagaccgcg aaatccaccc agtccatggc gaaaagactg aagttgaagc atcagttcat
     3481 ttttcatgac gactatgcgc tggcatcatt attagatggg ctttctggta cctggcaagc
     3541 ggaagaatta tccaaaatct ggcgcaatct ggttgccaag gacaccaaag gtatgctgca
     3601 aaaaacgctg cagaattatt ttgatcataa ttgtgatctt tcgcaaacag cgactggctt
     3661 gcatattcat gtcaatacat tacgctatcg actgcagaga gtggaagata ttacaggaat
     3721 acaaatcaat aacttaaagc agttattctg gctgtatatc ggcatggagt tacaacgctg
     3781 aactgtattt ccctccaaat ttttcgccgt ctgacgtcgt atttttagcc gatacacaca
     3841 agtctaacgc ggctgctttc tccatactgc cccctgttat tttcatggag aaagcagaag
     3901 atggtttcaa taacagcaac tggcgccatc atcgcactcg tggtggcgat cgttttaatt
     3961 ttacgcaaag tctcgccggc ttacggcatg attgtcggtg cgctggtagg cggtctggtc
     4021 ggcggcgccg atctggtgca aaccgtgacg ctaatggtca ctggcgcgca aggcattacc
     4081 aatgccgtgc tgcgcattct cgcagcaggt gtactggccg gcgtgttgat agaatccggc
     4141 gcggcaaaca ccatcgctga aactgtggtg aaaaaagtgg gggaaagccg ggcgctttta
     4201 gccttagcta ttgcgacgat gattctgacc gccgtcggag tatttatcga cgtggcggtt
     4261 atcactgttg cgccgattgc cctgtctatt gcccgtcgtg ccggtttgtc gaaactcgcc
     4321 attctgctgg cgatgattgg cggtggtaaa gcgggtaatg ttatgtcgcc taaccccaat
     4381 gctatcgccg tttcggacac ctttcatgtt cccctcacct ccgtgatgat gaccgggata
     4441 atccccggtc tgttcggcct gatcgtggct tatttcctgg caaaatgtct cagccagaaa
     4501 ggcctgatgg tgatgcccga cgaaatgatt tcacacgaca accacggcaa gcagccggga
     4561 tttcttgccg ctatcagcgc gccgctcgtc gccattgtgc tgctggcgct acgacctgtt
     4621 gcgggtatcg tcatcgatcc gctgattgcc ctgcccgtcg gtgggttagc cggtgcgatc
     4681 cttatgggca ggtttaagca aagtaacgcc tttatgatgt ccgggcttac gcgcatggcg
     4741 ccggtagcca tcatgctgct cggtaccggt acgctcgccg ggattatcgc caactcggaa
     4801 ctgaaagatg tgctcattca tggactgacc tcttccggtc tgccttctta tcttctggcc
     4861 cctgtttccg gcgcgatcat gtccatggca acagcctcca ccacggcggg aaccgctgtt
     4921 gccgccgggg tattcagcca gacgctgctg gggcttggcg tatcagccct tgccggttcc
     4981 gccatgattc atgctggcgc aaccgtactt gatcacctgc ctcacggcag cttcttccac
     5041 gccacgggcg gcagtgtcgg catgctgatc catgaacggt taaaactgct gccttatgaa
     5101 acccttgtcg gttttgtggt cgccctgatt tccacactga tgtttggcgt cttcggactt
     5161 gccgggtaaa gacacgatga aaatagtcat tgcaccagat tcatttaaag aaagtttatc
     5221 ggccatgcag gtggccaccg ctattgagca gggatttcgt gaaatttatc ccgaggccga
     5281 gtatgtcaaa ttgccgatgg ctgacggcgg tgagggtacc gtcgaatcaa tggttgaagc
     5341 aaccgaaggc cattatctgt tccagaatgt caccggaccg ctggggacgc cagtacaggc
     5401 gcgctggggt atgctgggag atgggaaaac ggcgatcgtt gaaatggcct ccgcatccgg
     5461 actacatcat gttcctccag aatgccgtaa ccctatgctt accaccagtt atggcacagg
     5521 cgaactgatc cttgccgcac tggaccacgg cgttgagcga attattctcg ggattggcgg
     5581 tagcgccacc aatgacggcg gcgcaggtat gctacaggca cttggtgtgg cgatgcttga
     5641 tacaaacggg aaatcgcttc ctttcggcgg cggcgcgcta agcaacctgg cgctcatcga
     5701 tgcctcaggc tgcgacgctc gactgaaaaa cgtttctgtc accgtcgcct gcgacgttaa
     5761 caatccactc tgtggcgcaa caggagcgtc agccatcttc ggcccgcaaa aaggagcaac
     5821 accagaaatg gttgcctcct tagaagctgc gctgaccaac ttcggtatac gacttcagga
     5881 cgcaaccgga cgaaacgtga tgacgacgcc tggcgcaggt gccgctggcg gcatgggcgc
     5941 ggcgctgtta gggattctga atgcggagtt aaagccggga gtggagattg ttattgacgc
     6001 gctgcatctc gatagcctgg tcgcggatgc cagcctggta atcaccggtg aaggtcgtct
     6061 tgatagccag agcatcagtg gtaaaacacc gattggtgtc gcacgctgcg ctaagcggta
     6121 tcagaaaccg gtgatcgcac tggcgggcgg cttaacgacc gaccaccaga tagtgcatga
     6181 gcacgggctg gacgcggtgt tctccgtgct gacgcaaatc gccactctgc cggaagcgct
     6241 acaggatgcg gaatacaacc tccgcgtttc ggcacgcaat attgccagcg tctggaaatt
     6301 atcacagcag cagatgcgct aatcttcagt caggccggca agcagtagct ggcctggata
     6361 ttctttctca ccaggcccat gctaaaacag cctgcttcag tcatcctacg caaggcacta
     6421 gcatggcaaa aaaagagggg agtagtggat cccaaaagac ggttaagcgg ttaaattgaa
     6481 tggtcaataa gggggctgtt tgggaaacga atctatggtc actcccgttt ttgcaacacc
     6541 gattttgacg ataagtgggc ttgcttgaat ctatccggcg tctgaatggg attttattcc
     6601 cgcgcctcga tgagttccgc gcctgatgaa cctcaagaaa atatacggct tcaatgagcc
     6661 tttccgtttc acaggttcct caacaggccg gtgggccgtt catatcatca ataccgtgat
     6721 gctgatactc cgtacccacc ggaaccgcac ctgatgcggt tccggcttaa gggctgtcag
     6781 gactcgcttt tggctgccat caacaaaccg gaagagatgc cggattatct cttccgtcaa
     6841 ctcggtttta atcagacctg gcatgagtgg aagcgggacg aacagcacag gcaacaacaa
     6901 ctccggcccg gacactaccg gggcatgagt atgtaatgtc agggacagct atacgccata
     6961 aaaaaatgaa atagatggcc tctctataaa gaggccatga tatttactaa ccctgttcgt
     7021 taatacattc tataaagttt ttatgtaggc tatctatatt ttctcgggta tgaatagctg
     7081 atattgcaca acgtagaatt gcctttccct cttccactgt aggatacata gcaacggttg
     7141 tagcaaaacc acgatcatgc atttttttgc atatcgttat agctttttct tcattgccaa
     7201 ttaacaatcc acgtattggc attgtagtcc ctttgtttat gacttcggtt tcattaaatt
     7261 tgtcatcaaa gtattttata acttgttgta agtcattttg acgcaaggat actgtgccat
     7321 cgaggtgtaa atctgctgct gcgatacatg cagcaattcc tggtaatgat ggtggaccac
     7381 caaaagcata ggtagttcca tacgttttta taaagtttat ggtgtcagga taattgcatg
     7441 caataaatcc accatgagag ccaaacgctt tagataatga accagcgata ataattctgt
     7501 catcaagttt gttatccaga caagacatta catatccgga gccatttttc ccatatattg
     7561 acatgccatg agcatcatca atgtaagcat acccatcaaa tctgttagcc attctaacaa
     7621 taaattcgac aggagccacc ccacccatcg aaccgacact atcagatata gatattggta
     7681 ccatattatt tattgaagca tatttaaacg cttcttctat atcattttta tgttgaaagt
     7741 caaccctgat tgtttcaccg aactgttgta atagtcctct atttatttgc aatgttgcat
     7801 gcgctgtttt ctctaaaata aa
//
LOCUS       DAZGAP010000204         7787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGAP010000204 DAZGAP010000000
VERSION     DAZGAP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.205"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..1095
                     /locus_tag="ACHLAM_004442"
     CDS             37..1095
                     /locus_tag="ACHLAM_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO7855878.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKLFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNNQWFEEHRTAIESTGATLT
                     TGATGIDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTRLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSALPMAVFNHPEHRLVSPQLYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNQGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNLLN"
     gene            1105..2247
                     /locus_tag="ACHLAM_004443"
     CDS             1105..2247
                     /locus_tag="ACHLAM_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO7855879.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            2240..3013
                     /locus_tag="ACHLAM_004444"
     CDS             2240..3013
                     /locus_tag="ACHLAM_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855880.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3015..4094
                     /locus_tag="ACHLAM_004445"
     CDS             3015..4094
                     /locus_tag="ACHLAM_004445"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO7855881.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            4094..5050
                     /locus_tag="ACHLAM_004446"
     CDS             4094..5050
                     /locus_tag="ACHLAM_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO7855882.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            5061..6156
                     /locus_tag="ACHLAM_004447"
                     /pseudo
     CDS             5061..6156
                     /locus_tag="ACHLAM_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6174..6641
                     /gene="terW"
                     /locus_tag="ACHLAM_004448"
     CDS             6174..6641
                     /gene="terW"
                     /locus_tag="ACHLAM_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO7855883.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7010..7663
                     /locus_tag="ACHLAM_004449"
                     /pseudo
     CDS             7010..7663
                     /locus_tag="ACHLAM_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         1979 a         1931 c         1982 g         1895 t
ORIGIN      
        1 acaataaatt ttcttgccgt tccggataat aaaaaaatga acaaaaaaat ttggtttatg
       61 gaaggtttgt cctctcagag agatattatt cagggggtta aattatttgc acaaaaaaat
      121 aattttgcca ttaccgtttt tgcctcccac cgtaacgaaa gaaatgaaat cctttccgtt
      181 gccgattatt ctttgactga acctgaagat cctcaaaaac gtcttcagtt tattcaggaa
      241 accattcaga cctacggcat ccaccatatt catactggtc gtaacaacca gtggtttgaa
      301 gaacaccgaa ccgccattga atcgaccggt gccaccctta ctaccggtgc aacgggcatc
      361 gactggttaa ctctggctga cgaaaaagtt acttttgctc agtttatgga gcaaaagggg
      421 ctcccggtcg taccatcctg gcgggtgaat acgctggcag aattaaagac acgcctcgcg
      481 gctccgccgt tcactgacag tccggtatgc gtgaagccgg tgacgggtat ctacggcatg
      541 ggattctggc gctttgatga cagtgctttg cctatggccg tctttaatca ccccgaacat
      601 cgtctggtca gcccgcaact gtatattgct gcagcgtcgg cttctgagtc gtttaaaccc
      661 cttgttttga tgccgtacct gcctggcccg gaattttccg tcgatatcct cgcggataag
      721 ggcgaaatac tcgcagccgt gggacgccgt aaggaagggg ctatccagta tctggtaaac
      781 caaggaagcg cctgggaact ggcgtgtgac tgcgcccggg ttatgaaggc cgacgggctg
      841 gtgaatgttc agacgcgaaa cgatgtgaat ggcaacccgg tgctgcttga aaccaacatg
      901 cgtccgtcag gcggggtggg ttataccctt cacagcgggg ttaaccttcc cgggttgttt
      961 gctgctttta agctcggtct gatgtctgaa gatgcggtga tccagagcgc cagaaatacc
     1021 ttttctccgg ttgcagtgag atccatcaca gatgtcatcg tttacccaga atcactctct
     1081 aaccttctga attaaggcgt gtttatgtct gataccctcg aagatatgat ttatcgccgt
     1141 accctctcat gcggtacgat tcaggtaacc cgcgaccagg gtgaagtttc gctcgatgac
     1201 ctgtttgata tcgctgaaag acgtaacccg aaacgcgcct ttctgtttgt cagcaaagta
     1261 ctcggcaggc acattcccgt gccaccctca gtcatgcggc aggcctacag acagcttgcc
     1321 agccagttcc cctcaacgct gacagggccc atactgttta tcggtatggc agaaaccgcc
     1381 gttgggcttg gggctggtgt gtttgatgaa gtgcgccacc agcatcgtga atctgtctat
     1441 ctgacctcta cccgtcaccc ggttgatggc acattgcttt gcgagttcaa ggaagaacac
     1501 agccacgcca ccgatcatct gatctatctg ccagacgatg aagagaaaag acgtcgtgtt
     1561 acaaacgcgc gaactctggt tttgattgat gacgaagcaa ccaccggtaa tacctttatt
     1621 aacctgcttt ctgccctgcg taataccggc aagcttcaac atattgaaca ggttatcgct
     1681 gttacgctta cggactggag cgaaaatgcc ctgtccaggc gcagcacttt acccgtcact
     1741 tcggtttctc ttgtaagtgg tcagtgggga tggactccat taccagatgc acctgtcccg
     1801 gacatgccga aggtcaacgt aacttcacgt ggaaaatggg acatccaggg aaaacagtcc
     1861 tggggtcgac tggggatgct cgcacctgcg gccgatctcg gtcatgaggt atccttccgc
     1921 aagggggagc gtgttctggt tctcgggaca ggggaattcg tctgggagcc gttcctgctt
     1981 gctgaacggc tcgaagctgc cggagcacag gcattttatg gtgcgaccac ccgctcccct
     2041 atcgccgttg gttatgccat taagtccgca atttccttta cggataacta cggcctgggc
     2101 atccccaatt ttgtctataa cgtcgcccat cagcaatttg accgcattct tgtgtgtact
     2161 gagacacctg cagaaagtat tgacacgcgg cttcttaaag cgctggctga ggttgcaccc
     2221 gtcgtggaga ttgttaccta tgaataaacc cgttgttctg agcgaccttg acgacacgct
     2281 ctttcagacc cgtcgtaaaa tggtagacga gctggccctg gagccattcc gtaccggtgc
     2341 cgttgatcgt actctgaatc cacgaagctt tatgacagaa gaacagtcca tgttagtgga
     2401 ctggctccta gagcaggctg aactcattcc cgttaccgca cggggaactg aagaaatcag
     2461 ccgcgtaagg atccctttcc actcctgggc aatcaccacg cacggggccg tcattctcac
     2521 gcctgaaggc aaacccgacg aggagtggaa ggcccatatg ctcggccagc taactcccta
     2581 ccaggaaaag ctggcatcga tgcagcgtct gatcactgaa atgatggacg caaaagggat
     2641 caatgcctgg gcaagactga acttcgaata cggtgaaacg gcggtttata tggtgatgaa
     2701 acaccgcgac agcactcgcc ttgacgaact caatgccatt gcagatgaga tagaaacggt
     2761 gttttcgacc gagggcttct acatccaccg caacagtaac aacgtagcct ggcttcccac
     2821 cccggttgag aaagggctgg ccgtcagctg gctgcttgaa aaacttcggg ctgaacgcgg
     2881 agttttcccc gtaattggtc tgggcgacag cctgagcgat catcgtttta tgaaactgtg
     2941 cagctggttt ggcatcccgc gtcagagtca gtttgcagat gccatttcac agcgaatttt
     3001 tggagaaaat taaaatgcag ccacatgaaa catttaccgg ctcataccag cccggtgacg
     3061 tggaatttct gctaaagccg gttgtcattg aaatgacgcc agttgatcaa aaagaagagc
     3121 tgattcagtc cgggaagaaa cattattcgg acatgctcag ccaggagcca gcgccgacac
     3181 aatggcatct tgatttgttt caccgggcgc tggatcgggg cgcagagaga cttgcaaaag
     3241 aagtcacaca gctcgctatt gctctggccg aacgcttcgg tgatgagccc atagtactgg
     3301 ccagtcttgt cagggctggc gtaccgctcg gcgttatgct acaccaggct ctgcgtgaca
     3361 tgggaaaaac ctcatggcat tacggcatca gcatcatccg ggatcgtgga attgacggta
     3421 cagcacttga tatcattgaa gagcggcatg gtaccagcgg cattgtcttt gttgacggat
     3481 ggacaggtaa aggcgcaatt accggagagc ttgtacgcgc actgagagat cgcccgggct
     3541 atcctgagca gccgcgactg gttgtcctgg ccgatccctg tggctgttcc tggcttgcag
     3601 caagtgatga tgactggctc attccttttg gcatcatggg ggctccggtg tcaggcctgc
     3661 tctcccggtc agtatggtcc tctgaaggat tacatgggtg catggtttgc gagcatctca
     3721 gtgaattcga atgcagccgg atgcttgtcg ataccgtcgc tcatttccgt aagaagttaa
     3781 caccgtcatc cctcgctcct ctgagctgga atacggagtc aacccgggtc ttatggcaga
     3841 caagtcgcga cgttatcgcg ttcctggccg atgaatttaa agtggacagc gtcaaccgta
     3901 ttaaacccgg tattgctgaa gcaacccggg ctgtattacg tcgggtaccg gaccatgtat
     3961 ttgtgcgttc gattgacgac ccggacgttg ccttacttgt ggggcttgct cgtgaaaagg
     4021 gaataattgt tacagaaatg gggggaaccc tcggccagta tcgggctgta accattatca
     4081 aaaaggtatt ctaatgaaaa atcgtctttc tccctggaat cttggtgcca ctctatacat
     4141 gcctgcaaca cgggaagata ttgctgatgc cgttctgcac gagaaaatcc cgggtttgcg
     4201 ctctctggtg atttgcctgg aggatgctgt cagtgaagca gacatccccg tcgccctcaa
     4261 aaatctagag cacctgctgc acgaactcag taacagcatg cgtagcctgg gtaaaaataa
     4321 ctggcccctg ttgttcattc gcccccggca tgccgaaatg ggcaaatggt taaaagaaca
     4381 ttatgatctt tccgctgtcg acgggtttgt gttaccgaaa tttaccctaa gttcgttagc
     4441 tgaatggtgg gatatcatgg gcggaactca cctgagcatg atgccaacgc tggaaacaga
     4501 aaacgtcttt gatgtgattc agatgcgcga gctggccacc cggctgttgg aacatccctg
     4561 ccacaagcgc attattgcgc ttcgaatcgg cggcaacgat cttatgaacg tcgtgtcgct
     4621 tcgccgtccc cgagccctga cgctgtatga cagtccgatg ggctacgtca tcaaaatgct
     4681 cgtttcagtc ttcagcccgc gtgattttgc cctgaccgcg cccgtatgtg agcatattga
     4741 cgatcatgcc gttatggcca gagagctggc tcttgatatg gcacatggac ttgttggaaa
     4801 aacggccatt catccagatc agatagaggt cattcaaaac gcgctgatgg tcactcaggg
     4861 tgagtactct gacgctctgc ggatcctgaa ctctacccag gccgtgttta agtcgcaggg
     4921 ggcgatgtgt gaacctgcca cacatcgccg ctgggcggct ggcattctgg acagagcccg
     4981 gttttacggg ttacataacg agcaaagcgc tgatggaatc agattactta ctataaccca
     5041 gcatcattaa ggaaatcata atggggttca ggttcagagg tacttccgtt tctttctgta
     5101 gccggggggc ctatgccaat ctggggttac ccggtaccga gctgagttac cgtacccggt
     5161 ttgagctggc cgcgccttcc ggaggtggaa acaggacagc aaccgtcccg gatctcagac
     5221 agtcgcttga gcaggaagca gttgaactca tgtcagcggt gaccgcaatt cgtaacattc
     5281 acgagttgac gctggatcca aaaacaggca tcagttgggc agagctggaa gcagtatacc
     5341 tgcatagcag aacatcgctt tttcaggttc cggcaccagt gcgtcctgaa aaaccagaat
     5401 accatgcatt gtcggaaaat cctgcaggga acaaaggcat tgtttttctg ggtaaatggt
     5461 ttgaatcgga atcagctaaa gctgagcgtc aagcagggaa tcttcgccga tggcggcaag
     5521 aactgattga tgtgaagcgt gagaatcccc ttcgacagca ccggtaccag caacggcaga
     5581 cgaccagggc cgaacagtat tcaaactgga agtgtgatgc tgaagaacat gaaaaaaggc
     5641 tcgccacgac tcaggcagat gcccggcagt tgttccgggc agacgcagcg tttttcgaat
     5701 cataccttgc gggtgtgctt tcagaaactg aatggccgcg tgaaacgctt gttgcatttg
     5761 aagtaaagcc ggagatatca acagtcctgc cggatgttgg tttagctgag attgaagatt
     5821 tccctgataa gatttacggc gtcaatgccc ggggcatgga gctgacgcaa aaagccatac
     5881 gcgaaaacta tgcccgccac agccatggct gcttgttccg actggtcggt atcgtgttac
     5941 atacgctacc tttcgacaag gtgatcgcat caggatttac acaacgagta agcggaacga
     6001 ctatctggag gatgtttata tcctgtcctg caaatgcact cgcagccaga aggcatagaa
     6061 catattgatc cggttgaagc gttaggctat caccctgtta ttcgaaagat gagaaacaca
     6121 ttcatttttc agcctattga accactaacc ctttaagcaa actaaatatt acgatgcaat
     6181 taaataccag acaggccaga atttttaagc ttgccaactt actgggctcc ggcaagcctg
     6241 tttccgctac cgacattata acctatctgg actgctcaga gccgacatta acacgggccc
     6301 tcaaagagct acgaaagtca tattctgcag aaatcaaata cagtaaggca ggtcattcct
     6361 atcatctcgt taatccgggt caactggata aaaagacact tcgccggatg aatgaggcgc
     6421 ttgcacagaa tgctgatctg aaaaccggtg agtctacagg aaaggtcgta ctcgataagg
     6481 ataaaaaaac agccgtatca ttatcgctac gcatgcgaat tttaagaaaa attgaccgtc
     6541 tggctgcact gagtggatcg acccgaagtg aagctgttga aaaactggca ctgcattccg
     6601 ttgacgaact gataaaagaa tacagcgcta aaaaatcctg atgacaaatc tcagctcggg
     6661 gtgaataccc cggttttttc atatttcaac tctgtcttgg cgtgacgtat ttttgatcta
     6721 ttctcctccc gcaatgtcag cagctcctta cagcctacaa aacctagcgt cttgacatcc
     6781 tcttgtgcta cgcatcaatc tttgggcgat ttacactcca ctcatgcaaa tctgtgatgt
     6841 atgatgattt tcagtaagtg taaactgact gccgtatgta gccattaagc cagtattggt
     6901 aacgtagatg cccacctttt cacttatttg gtggacacca ctttgtctaa ctcgtcagat
     6961 tctgaccaaa cgtttcaggc tgtacgctta ctctgaaggg gggagaatag tagcccgtga
     7021 tagcattgag ctaactgaca gccagatcgc agcactaaag cgtaaagcca gtgatgacga
     7081 ggcctgtggc gaaagcgaaa cggctcatcc gggatatctg ggttcgcagg acacgttcta
     7141 tgtaggcaac ctgaaaggtg tcgggcgtat ttatcagcag acgttcgttg atacgtactc
     7201 gaaagtggct cactgtaagc tttacgtcac caaaacaccg attaccgcgg ctgacttact
     7261 gaatgatcgt gtactgccgt tttatacgtc tcagggcctg ccgatgctga gaatactgac
     7321 cgacagaggc acggagtact gtggcaaagt agagcagcat gattatcagc tttatctggc
     7381 aataaacgat atagaacaca cgaaaacaaa ggcgatgtcc ccgcagacca acggcatctg
     7441 cgagcggttc cataaaacaa tcctgaacga attttatcag gtaacgttcc gtaaaaaact
     7501 gtacggcgat cctgatacat tacagtcgga tcttgatgaa tggctggctc actataataa
     7561 tgagcgaagc catcagagaa aaatgtgcaa tgggcggacg ccaatggaaa cgttacttga
     7621 tggaaagcgc atctgggccg agaaaaattt aagccagatg taatctgaca gatacctgta
     7681 taaataaccg gtaactgtca gatcaggtct gagctaatac aaattaaggt tttaaattca
     7741 ttaagtaatc aatatcaact ggatgattat aatccactga ctcaaat
//
LOCUS       DAZGAP010000205         7738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGAP010000205 DAZGAP010000000
VERSION     DAZGAP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.206"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..933)
                     /gene="fbpC"
                     /locus_tag="ACHLAM_004450"
     CDS             complement(<1..933)
                     /gene="fbpC"
                     /locus_tag="ACHLAM_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO7855884.1"
                     /translation="MSQKNFVELRNVTKRFGSNRVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKDALTMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTAGVR
                     PEAITLNNHGEESQRCVIRHVAYMGPQYEVTVEWH"
     gene            complement(945..3023)
                     /locus_tag="ACHLAM_004451"
     CDS             complement(945..3023)
                     /locus_tag="ACHLAM_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7855885.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMNVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILTVWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPLYLAGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(3092..4123)
                     /locus_tag="ACHLAM_004452"
     CDS             complement(3092..4123)
                     /locus_tag="ACHLAM_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7855886.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(4120..5436)
                     /gene="uhpC"
                     /locus_tag="ACHLAM_004453"
     CDS             complement(4120..5436)
                     /gene="uhpC"
                     /locus_tag="ACHLAM_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO7855887.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLNANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(5509..7050)
                     /locus_tag="ACHLAM_004454"
     CDS             complement(5509..7050)
                     /locus_tag="ACHLAM_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO7855888.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALLILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPAILAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(7050..7679)
                     /locus_tag="ACHLAM_004455"
     CDS             complement(7050..7679)
                     /locus_tag="ACHLAM_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO7855889.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWPALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
BASE COUNT         1929 a         2133 c         2038 g         1638 t
ORIGIN      
        1 atgccattct acggtcactt cgtactgcgg ccccatgtaa gcgacatggc gaataacaca
       61 acgctgactc tcttcgccgt gattgtttaa ggtgatggct tccgggcgaa cgccggcggt
      121 gccgctacct tgcgccggga aatgcgctgc gcgcggcagg cgataaccgt agatatcgac
      181 atactcttcg ctaaagttcg ccgggaacag gttggcgtcg cccataaaac ttgccataaa
      241 tcgggaggct ggctgacgat agagatcctg cggtgagcca atctgcatga tgtgaccttt
      301 attcatcacc agcacagtgt cggacaccgc gaaagcttca ctttgatcgt gagtcacgta
      361 gagtgacgtg atattaaact gcttttgcag ctcgcggatc ttatcgcgca tgctgcggcg
      421 caggttggca tcgaggttac ttaacggctc atcaaacagc agcacctttg gcttgaggat
      481 caacgcacgg gccagagcca cacgctgttg ctgaccaccg gaaatctggt cgacatagcg
      541 gtcttcgaat ccttccagat ccaccattgt cagcgcatct ttcacccgct gcttcacttc
      601 actacgcgac acgccaagca ttttcagccc atagccaacg ttttcgccca gcgacatatg
      661 cgggaacaga gcgtatgact gaaacaccat gcagatatcg cgttgctgaa tggaacgatg
      721 cgtgacatct tcgccatcta taaaaatttg cccttcgctc ggtttttcca gcccggcaac
      781 cagacgcaga atggtggttt tgccgcatcc ggaaggaccg agcagcgtca ccatttgccc
      841 ttgtgggatg gtgagattga tattgtcgat aaccctatta ctgccgaatc gtttagtgac
      901 gttgcgcagt tccacaaaat ttttctgact catagtgcgc tccattacgc ctggtttttg
      961 gctttagaac gggaaatacg tgcttcaccg atcagccagt caaagaggaa aataatcgcc
     1021 agcatcacca cgatcagaat cgaaccgtag gcgatggcta cgccatattc gccgtcttct
     1081 acgcggttaa ggatgtaggc ggttgccacg cgtgtgtccg gtgtgacgag aaagacgatg
     1141 gcgctgactg tagtgatggc ccgcacaaag ctgtaaatca gggccgagag aatggccggg
     1201 cgtaacagcg gcagtaaaat gtgcgtaatg gttcgcaggg aacccgcacg caggctaagt
     1261 gaggcttcat ccagcgattt gtcgatctgc cccagacctg caatcccggc gcgaataccc
     1321 accggaacgt tacgcatcac catcgagata ataacaattg ctgccgttcc ggcgagatag
     1381 agtggggcgc tattaaaggc aagaatatac gagacacccg ccactgtacc cggtacggca
     1441 aagcacagca tggtggtgaa ctcgatggtc tttttgcctt tgaattgttg acgcaccacc
     1501 acccaggcga tgagcaggcc aaagatagcg gtaatggggg ctgcaatccc ggcgtagagc
     1561 agggtatcca gcagtgaagg ccacgcgccg tcgctcatcc cctgaccaaa taacttgata
     1621 aagttatcca gcgtcagggt gtagtcaacg ccccagttca ccgtgaagct gccgtagaaa
     1681 atgctgccgt agagcagggc gttaaaggca atccataccg taagtatggc gaccacgctc
     1741 cacaccaggg tgaccggtag cggctggacg tcgccacggt aggatttgcc ggaaaccgtg
     1801 acgtaagagc gtttaccaat ccacatatac tggacgcaga acaccagcag cgagaacagc
     1861 agcaggaaag caccaagcgt gctggcggcc tgataatcca gctgcgagcc ggtaatgtag
     1921 aagtaaattt gcgtcgccag tacgtcgaag ttgccgccaa gcaccagcgg gttactgaaa
     1981 tcagccagtg actgcaccac gacgattaaa aaggcattgg cgagcgcggg ctttagcaat
     2041 ggcacaaaca cgccgttaaa ggtttgccag cggctggcgc gcagggtata ggaggcttct
     2101 tccagcgagg gatgaatggt tttaatggcc ccgtcgagga tcataaaagc cattggcgtg
     2161 aatgccagaa cctgcgccag ccagatcccg gtaaaaccat acagccagtt ggtgttggtc
     2221 agcccaaacc actccaccat aaactccgtt atgtagccgg agcgccccat catgagcgtc
     2281 acgcctaagc cgaccacaaa aggtggcgta acgatgggca gaatggaaaa aatgcggcca
     2341 ataatggcgc tgcgtttggc gatgcgcgtg gtataaatcg ccagcaccag gccgaagaag
     2401 gtacagccaa tacccaccgc tatcgacaag acgatagagt tgacgatcac ctgcacaata
     2461 tgtgcctgcg agagcacgtt cataaacgcc agcggggcga actctcccgc gtcgttggtg
     2521 aacatcggga taaaaatggc gatgctcggc cagacgataa aaatgccaat cagcgccaca
     2581 atcgcaatca gtgagccgat cacgaagcgg tcgccgccca gccattcgag gcgggttaag
     2641 gccagcgtca taatcgcgcc taattcaaca aacagcacga tggcggcgta gcctaaaccg
     2701 cgtccagcca cggtggcgct gatgacgata aacgccatac agagcaatgc ccagctggca
     2761 tcaaaataat gacgactacg ctgttcgcgt gtggcctcac gaaacggacg aaccagcaac
     2821 agactcggta ataaatacca cagccagctg atattaaaat gcgaccagcc ataagcctcg
     2881 aggatctcat cactcgtgga ctccagcagg ccataatcaa ggctccagct cggcaacagg
     2941 ataaatgcca gccagcccag caaaacccaa aggaaaatcg catcccgctt tttcacggga
     3001 tggagtgcaa gtgtctgtga catagcagtt ccggtatggt tggccggggc aggcgtttgt
     3061 gctgcacccg gcaaatgcgc ctaatgcggg tttatttacc catcttcact tcgctgaccc
     3121 atttattaat caatgcctta cgaacatccg tggaaccata tttatccatg tcgtagttga
     3181 ttaatttcag gtcgtcgagc ttcagcgaat ttggcgacgt ttcggcggtg gtattggtca
     3241 ggatctgata agacttccct ttcttccacg ccagttcctg agcttctttt gataataccc
     3301 agtccacaaa cagtttggcg ttgtcgaggt tgcgcgcgcc tttcagaatg ctgacgccgc
     3361 caatttcgta gcccgtacct tcacacgggg aaatcaactc cagcggcgcg ccctgttctt
     3421 tttccagcga gtagtcatgc aggaagccaa taccaatcgc cgtttcgcca cgggcggcgt
     3481 tacgtgccgg ggctatgcct gatttggtgt actgggagac gttggcgttc agttgtttta
     3541 gataatcaaa ggcctgatct tccccccaca gctgcgcaaa agtagccagg gcggtgtagg
     3601 cagtaccgga actttgcgga tcggcaattt gaatttcgcc tttatattcc ggtttggtga
     3661 gatctttcca gcacttcggc accggcaggt ttttctcttt cagacgatcg gtgtttacgc
     3721 caaaaccgag gatccccaca tatacggcag aggacagatt ccctttcact tttgccggat
     3781 cgcggaattt ctccatgatc tgctcaaggt taggggattt atacgcctgt agcaggccca
     3841 tctcgccagc ctgcgattgc gggtctaaag tgccgccgta ccagacgtca gcctgggggt
     3901 ttttcttttc agcatcgact tttgccaggg tgcttccgga accgttgcgg ataaacgagg
     3961 tttttacatc atatttctcg ccaaacgctt tggtttcggc ctcacacatt tcattggtgg
     4021 cgctgcaata gaccaccagc cggcctttcg cctgcgcggc acccgttaaa gtcgccagcg
     4081 caacggcaga agcaatcaga gtagaggtaa aggtcatttt cattgtttta tccttcgagc
     4141 gtgagtggtg gtgatgcccg ccatcagtaa cggcatcagc agtaaaccca taagtactgc
     4201 ggcgaccgag agtaaactga acattcccga ccagccgtag cgttcgatga ccagcgacag
     4261 tggccagccc gcaagcgccg cgccaaggta agcgaacagg ccgagaaatc cggtgattga
     4321 acccgccgca gctttgtgcc cacactccac cgccgcaagt ccaatcagca tttgtggacc
     4381 aaagacgaaa aagcccacgg taaaaaagca cactgccagc agagcgtagt gatgaaccgg
     4441 tgccagccac agtgcggcga ctgacaccat caaccccagc gtgaacagca aaatcattgg
     4501 cgctcgttgc ccgctaaaca gcaaatccga tccccatccg gcaaataacg cgccgagtaa
     4561 gccgcccact tcaaacagca ttaccgtggc gttggcgttg agcaggttga cgccgtggct
     4621 ttccgtcagc cagatattgc cccagtcgtt aagcgcaatc cggatcacat aaaccagtac
     4681 ataagagacg cccagtagcc agatcagcgg attttgcagc atggtggtgc gcaacatttg
     4741 ccacagcccc atcggcggac tttgttgttc ctgacgtaac tccagcggat cgtgacgcca
     4801 gtgaccaact gtcggtaatc cctcttcctg gggcgtgcct ttgagttgta gcgttaacca
     4861 gatccctaat gccatgctga ttatccccgg tgtcagcatc gcggcctgcc agccccacca
     4921 gtgggcggca aaggcgctaa tcagcggaat aatcgctccg ccaatgttga tcgacatatt
     4981 ccagcagccc caccagaaac cgcgctcatt gcgtgagtac cagtgggtca gcaagcgggc
     5041 acacggtggc catccccagc cctgaaaaaa accgttcagt gtccagacca caagcagaag
     5101 cgtaagtgat tcgccaaagg caaacaccac gttcaataac ccggtggcga acaagccgac
     5161 gcccataaaa ccgcgttgtc cgtggctgtc atgccacagt ccggcggtaa atttcgacaa
     5221 tccgtagctg agataaaaca gcgaacccag caggccaatg tcgcctttat ccagtccgag
     5281 gtcggtttgc agcgccggaa ggacgtaatt cacgcttttt cgcgtcagat aaaatgcggc
     5341 gtagccaata atcatgcaca tcagcaatcg cggacgtaat gtccggtagc attgattaat
     5401 ttctctcgct gaacgcgcgt gcatggttgt ctccatgttg ctgactatat gaaaatgaaa
     5461 gtgaaagggg atgagagaaa atccgggata cctaagactt tttcctggtt atccggcgat
     5521 ttgttgcaaa aatgtgggca agttaacaat tacccgcgtg ccgtgctgtt tttcgagcgt
     5581 taagtcgccg cccagggcgc tgacgcgctc acgcatcccc tgaatgccaa atccggccat
     5641 tttcccgctg gcgatcccca caccgttgtc gctgacctcc agatgcaata cctctccctg
     5701 ctggcgcagg ataatcgtca cttcgctggc ttcggcgtgt ttacagacgt tattgagcaa
     5761 ctcctgcaat aaccgataca aggtaaagcg caccgtttcg ttttctggtg tggagttcag
     5821 ttgataggca aactggcaat gaatgccgcg ttcactaaag gcaaattcat taatcagatg
     5881 cagcaacgct tcgcggaatg tcagttcatc aagcgcggga ggccgcaact ggcgcagcaa
     5941 ctggcgggtg gagagatgaa tgcggcgggc gagttcattg atttggctgg ctgcggattg
     6001 aatttgcgcc ggatcgcgcg cccgcttcac cagttgcgac tggatctgaa tggcggtgat
     6061 gttctgaccg atttcgtcgt gcagctctcg cgccaggttt ttgcgcgtgt cctcctcggt
     6121 gtggatcagc ttttccgcca atgcccgccg cgctgccagc tcggcctcca gccgttgtcg
     6181 gtaatggtgc aggtttagcg ccagatgttg ctggcgactg atggcaatgc ccaggccaat
     6241 tcccagcaat gcctgggtcg cgaggaaaat ctccagctcc accaggttac taaagcccac
     6301 gccaatctgg cgggcgatgg tgatcatcat gctgcccaaa agcccggaga gtacgccacc
     6361 ttgccagcca aatttccacg ccatcaccac attgggtaga aaaactacga tcaacagcag
     6421 gcgttcaatt tcaggtgaca gcaccatctg agtgccgata ccaatgataa aaaacaggct
     6481 acaccagatg attaaagagg tacgcaaggg gggattatgc gtgtcaagcc ccaggagctg
     6541 gtagcgatgc tgctggcgca gaaactcaaa gatgagatag acaaacggcg tcagtaatac
     6601 gccgccggta aatgaggtca accccggcag cattattggg cctgacataa aaggggagag
     6661 cagggcggta ttcaacaatg aagctgccgt gacagtggct aacagcaacg tcagacgctg
     6721 ccagtaaagt ggaaaacggt gccagaagtt ttgcgcaagg atggcaggaa acaggctaat
     6781 aaccggcgtt gccagcatga gataaccgtt caatagctgt tcactatgga gccagaacat
     6841 catcagcaaa ggtgggagca ccagcgcagg ccagtagcgg cgagagagca aaataagaag
     6901 ggcgaggtaa accccctgcg ggagaaacaa caccgcctgt tgaccgttat tggtcagata
     6961 aaagctgagc gtccacagca tcagccagcc agttccccag gccagcacaa taaacagaga
     7021 aatcacccaa tgacgaaggt tgcgcgacat taatgccccg ccagcagatg gtggtcgagc
     7081 gcaaaatgca ccagctcaat ggtactatgg cagttcagtt ttcccagcac attggcgcga
     7141 tgcacatgta ccgttttatg actgagttcg agcttaaagg cgatctcttt aacgctgtca
     7201 cctttgacca gtaaatcaaa cacttcacgt tctcgtgggg tgagaatttc cagcgcctgg
     7261 gcgggttgcc cgccgccgcg taaagcccga attgcatcgg cacagagata atgcccgccc
     7321 agcccgaccg agcgcaccgc ctgtactaac tcttccggcc cacagcgttt ggtcaaatag
     7381 ccgctggcac cagcatccag cgcgctttgc acaaaggttg gcgcgtcata aatgctaaga
     7441 ataatggcgc gaaattgcgg tttttgtgca cgcaggcgct taagcaaact gaggccgttt
     7501 tcgtccggca tagcaatatc aatgacggca acattgacgt catcacggat taatgccggc
     7561 catgcctgtg cggcactact gtactgacca atgacctcca gatcgtcttc cagagagagt
     7621 aattgtgcga agccagagcg caccaccaca tggtcatcca ccagcacaac ccgaatcata
     7681 cttttcttct cttattatct cattcttcta tcatgcatca cggcgcgtcg ctggcaat
//
LOCUS       DAZGAP010000206         7673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGAP010000206 DAZGAP010000000
VERSION     DAZGAP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.207"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..634
                     /locus_tag="ACHLAM_004456"
     CDS             53..634
                     /locus_tag="ACHLAM_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7855890.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            634..1791
                     /locus_tag="ACHLAM_004457"
     CDS             634..1791
                     /locus_tag="ACHLAM_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7855891.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            1814..2269
                     /locus_tag="ACHLAM_004458"
     CDS             1814..2269
                     /locus_tag="ACHLAM_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7855892.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            2288..3328
                     /gene="terC"
                     /locus_tag="ACHLAM_004459"
     CDS             2288..3328
                     /gene="terC"
                     /locus_tag="ACHLAM_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO7855893.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            3377..3955
                     /gene="terD"
                     /locus_tag="ACHLAM_004460"
     CDS             3377..3955
                     /gene="terD"
                     /locus_tag="ACHLAM_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO7855894.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            4023..4598
                     /locus_tag="ACHLAM_004461"
     CDS             4023..4598
                     /locus_tag="ACHLAM_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7855895.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            4712..6006
                     /locus_tag="ACHLAM_004462"
                     /pseudo
     CDS             4712..6006
                     /locus_tag="ACHLAM_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            6055..7263
                     /locus_tag="ACHLAM_004463"
     CDS             6055..7263
                     /locus_tag="ACHLAM_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO7855896.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMT
                     HHLGYEKNQSKPGTNSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTLITG
                     MDNQIFSLYAKGMTTREIAAAFKELYDADVSPALISKVTDAVMEQVVEWQNRPSDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELPGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPGAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRN
                     LKAIYQAPTEEAGQQAQEAFAASWDSRYPHISRSWQANWPNLATFFAYATGIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMPLKDWRMAMSRFII
                     DFGDRLGGHF"
     gene            complement(7378..>7673)
                     /locus_tag="ACHLAM_004464"
     CDS             complement(7378..>7673)
                     /locus_tag="ACHLAM_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7855897.1"
                     /translation="SRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGW
                     FIARGFRAERIHATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGETSVTD"
BASE COUNT         1866 a         1882 c         2057 g         1868 t
ORIGIN      
        1 tactaaatat acttttgatt gtctcagtta tcagcatttt aaggaataaa acatggtttc
       61 attagttaag aatcagacgg tatcactcag caaagaatca cctgcattaa gccagcttca
      121 tttcggtctc ggttgggatc cggttaagaa aaaaggtttt cttggaggat tgtttggagg
      181 taacgactca atcgatctcg atgctggctg tgtattaatg gacagcaccg gtaaaacaat
      241 tgacaccatc tggttccgca aactggaatc cacctgcaaa gctgttgtgc atagcggtga
      301 taacctgact ggtgagggtg acggcgacga tgaagtgatt aaggtcaacc tctcccgtct
      361 gcctgcaaac gttgaatacc tggcgtttac ggttaacagc ttccgtgggc agtcgttcaa
      421 tgacgtcgaa aatgcgttct gtcgtgtggt cgatcaaacc ggcaaagagc tggcttgcta
      481 caagctgact gagcagggct cacataccgg gatcgttatt tcttcgctgc gccgtaataa
      541 cggcaactgg gactttaccg cccttggtca tgcctgtcgc ggtcgcacaa ttgacgatat
      601 gcactccgat atcgtttctg cggttattcg ctaatgaacc tgatgccggg gggcaatgcc
      661 cccgttccag cccaagagtt gcgggtccgg ataacctcgg gcgtccaggt ggatgcttca
      721 gcgtttcgcc tgtatgccgc cgggaaggtg cagggggatg cggacatggt gttttatggt
      781 cagccacgta acgatgatgg tactgtcagc ctggtcagtg aaggtcagta ctcaaccttt
      841 actgtcgcgc tcaaccggct gaaacctgat gtccagaaaa ttgcgttcac cgtgacctgt
      901 gacggtgggc agacggtgtc cggtcttcgt aatctttcca tcgacgtgga gcaaggggct
      961 accggcctgg tgagcggctg tgtcgaactg agcggccgtc aggaagcagc cttaattctg
     1021 ggtgaatttt accgtcgtaa taatggctgg aaattccgct tcgttgcgca ggggttcaac
     1081 ggtggactta aaccgctggc agagcatttc ggcgtaaata ttgccgacga accggctccc
     1141 gcagccccga ctcctccggt tgtaccccct ctgccagctg agacaaaaat cagcctgagt
     1201 aaagtatctc tgacaaaaga gatgcctgca atcagtctga ccaaacggga taacttcggt
     1261 gaaatcagga tcaacctgaa ctggcaccgg ggcagcggca aatccggctt tgcaggcatg
     1321 ttcggctcca aaggcatcga cctggatctg ggagcctttg ttgagcttca ggacgggtat
     1381 aagtcggtca ttcaggcgct cggtaatgca ttcggtgatt atcgcgatga accttatgtt
     1441 cagctcaagg gcgatgaccg gacgggtgat gtatcagacg gcgagtggct gcatatcaat
     1501 ggacgtgaat ggaagcatat ccgcgaagtg ctgatttacg cctttattta tgaaggtgtt
     1561 cccagttggg acaagactga tggtgtggtc accatttatg tgccggatca accgcccatt
     1621 gagacccgcc tgactgaagg tgaaaaccgt cgcacattgt gcgccattgc cagactcgta
     1681 aacgaaaacg gtgcgattaa agtcgagcgt atcaaccagt acttcaaagg ccaggacgaa
     1741 atggaccggg catttggctg gggatttcgc tggagcgccg gttctaaata acacagcaat
     1801 aaaggaaact ggtatgagct ttttcgacaa agttaaaggt gccattaact ctggccgtga
     1861 cgaactgacc cgccaggttg gtcgtttcaa aaacaaaaaa ttcatgcagg gcaccgttgc
     1921 tgtatgtgcc cgtattgccg tatcgagtga cggcgtgagt tcggaagaaa agcagaaaat
     1981 gatgggcttt ctgcgctctt cagaagagct gaaggtcttc gataccaatg aggtgatcga
     2041 gttcttcaat aaactggttt caagcttcga tttcgatgtt gaaatcggca agggcgagac
     2101 catgaaatac atcctggcgc tgaaagatca gcctgaggcc gctcaactgg ccttacgtgt
     2161 tggtattgct gttgcgaaaa gcgacggtaa cttcgatcag gacgagaaac tggcctcccg
     2221 cgagatcgct atcgcgttgg gcttcgagcc ggctgaattt ggcctctgac atccctaagg
     2281 gtaaaatatg gtttccacac acatcggctt cccgactgaa acggtcattg tttttattgc
     2341 tctttcagtc ggagccatct ttattgacct gtttatgcac cgtgatgaca agccaatctc
     2401 cctgaagagt gcagcgctct ggtccgtatt ctgggttgtt gttgcgatgg catttgccgg
     2461 tttcctctat atccaccacg gtgctgaagt tgccagtttg tttgtcacgg ggtatgcgct
     2521 ggagaaagta ctgtcggtcg acaacctgtt tgtcatgatg gccatctttt cctggttcgc
     2581 tgttccggat cgttatcggc accgtgtgct ttactggggg atcattggag cgatcgtctt
     2641 caggggaatc tttgtcgcca tcggtacgag cctgctgagt ctggggccgt acgttgaagt
     2701 cgtcttcgct atcatcgttg cctggacagc ggtcatgatg cttaaaagcg gtgatgatga
     2761 tgatgaaatt gaagattact cccagcatct ggcttaccgc atggttaaac gtttcttccc
     2821 tgtctggccg aagctacgtg ggcatgcctt cctgcttaac cagaaggaag tggatgctga
     2881 actggcgaaa ccagaaaaca gtgatgtaac cattgaccgt ggtaaaaagg cggcgctgta
     2941 tgcgacccct ctgttcctgt gtgtggccgt ggttgaactc tcggatgtta tgtttgcgtt
     3001 tgactcggta ccggcaatca ttgctgtcag tcgtgaaccg cttatcgtct atagtgccat
     3061 gatgtttgct atcctggggc tgcgtactct gtactttgtc cttgaagcac tgaaacagta
     3121 cctgattcat ctggaaaagg ctgttatcgt gttgttgttc ttcattgcgg taaaactggg
     3181 cctgaacgcg accgatcaca tctggcatca tggttacagc atcgctgcga caaccagcct
     3241 gtatgttgta ctgggggttc tggtgctggg cattctcgca agcgttattt tccccggcaa
     3301 acctgaatct gaggaaaagg ggaactgatc cccttaagtg ttaaccggag caattactaa
     3361 tcgaagaggt tattttatga gtgtttctct ttccaaaggc gggaacgtct ccctgagtaa
     3421 agcagctccg tcaatgaaaa acgtcctggt gggtcttggc tgggatgcac gttcaactga
     3481 cggtcaggac tttgacttgg atgcttcagc attcctgctg accgcaaacg gcaaagtgcg
     3541 tggcgattca gatttcatct tctataacaa cctgacgtca tccgacggtt ctgtaacgca
     3601 caccggcgat aaccgtactg gtgaggggga tggtgatgat gaatcgctga aaattaaact
     3661 tgatactgtc ccgtctgaca tcgacaggat catcttcgtt gtgacgatcc atgatgctca
     3721 ggctcgccgt cagaactttg gtcaggtttc cggggcgttt attcgcctgg ttaatgacga
     3781 taaccagact gaagttgctc gctacgatct gactgaagat gcgtccactg aaaccgccat
     3841 gctgttcggt gaactgtatc gtcacaatgg tgagtggaaa ttccgtgctg taggtcaggg
     3901 ttatgcgggg gggctggcat ctgtatgtgc tcagtacggc attaacgcgt cttgatcgaa
     3961 aactaacttt tcattccggg ctgttgattc agccctcatt caactgtttg caggagctaa
     4021 aaatggcagt ttctctcgta aaaggcggca acatatctct gaccaaagaa gcaccgacca
     4081 tgaatgttgc tatggttggt cttggctggg atgcccgtgt aactgacggt caggcttttg
     4141 atctggacgc ttccgtattc gcagtaggtg aagacggtaa agtgctgtca gatgcccatt
     4201 tcattttctt caataataaa accagccctg atggcgcagt agagcaccag ggtgacaacc
     4261 gtaccggtga aggcgatggt gacgatgagc aaattaaaat cgatctggca aaagtctcag
     4321 ttgacgtcaa aaaactggta tttgccgtta ccatctatga tgcagaagcg cgtaaacaaa
     4381 atttcggcat ggtgagcaac agcttcatgc gcgtttacaa caacgataac ggcgcggaaa
     4441 ttgcccgttt cgatctgtct gaagacgcct caactgaaac cgcaatggtc ttcggtgaac
     4501 tgtatcgcca tggcgctgag tggaagttta aagctgtcgg tcagggtttt gccggtggcc
     4561 tgtcagcgct tgcttcccag cacggcgtca acgtctaaaa gatgcttagt ctgaccccgg
     4621 caatgccgta atgccgatcg gttaaggatc agttgaccga tccagtggtt gtgtaagaat
     4681 ccggaaacgc tcactcgttt ccggattttt tatgcacact ggacaggccc ttgatctggt
     4741 atcccgttac gattctctgc gtaacccact ggcttctctg ggggattacc tcgaccccga
     4801 actcatctct cgttgccttg ccgaatcagg tacggtaacg ctacgcaagc gccgtcttcc
     4861 cctcgaaatg atggtctggt gtattgttgg catggcgctt gagcgtaaag aaccccttca
     4921 ccagattgtg aatcgcctgg acatcatgct gccggcaatc gccccttcgt tgcccccagt
     4981 gccgttattc aggcccgcca gcacctggga agtgaggctg tccgccgcgt gttcacgaaa
     5041 acagcgcagc tctggcataa cgccacgccg catccgcact gatgcggcct gaccctgctg
     5101 gccatcgatg gtatgttctg gcgcacaccg gatacaccag agaacgatgc agccttcccc
     5161 cgccagatac atgccggcaa cccggcgctc tacccgcagg tcaaaatagt ctgccagatg
     5221 gaactgacca gccatctgct tacggctgag ccttcggcac gatgaagaac agcgaaaatg
     5281 agcttgctga gcaacttata gaacaaaccg gcgataacac cctgacgtta atggataaag
     5341 gttattactc actgggactg ttaaatgcct ggagcctggt gggagaacac cgccactgga
     5401 tgatccctct cagaaaggga gcgcaatatg aagagatcag aaaactgggt aaaggcgatc
     5461 atctggtgaa gctgaaaacc agtccgcagg cacgaaaaaa gtggccggga ctgggaaatg
     5521 aggtgacagc ccgcctgctg accgtgacgc gcaaaggaga agtctgccat ctgctgacgt
     5581 cgatgacgga cgccatgcgc ttccccgggg gagaaatggc ggatctgtac agtcatcgcc
     5641 gggaaattga actgggatac agggagataa aacagacgat gcaactgagc aggctgacgc
     5701 tgtaaaaagc cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg
     5761 gtaagatgtc agatgattaa aatggtggaa catctgaaag gttactggcc gaatcaactg
     5821 agtttctcag aatcatgcgg aatggtgatg acattgcagg gcgcttcacc gggacgtata
     5881 ccggggctga tgcgcgatct tgcaagtatg ggacaacttg tgaaattacc gacaagaagg
     5941 gaaaggggct tcccgagagt ggtaaaggag aggccttgga aagagcctgt acatagattt
     6001 gtgtaattgc ctgattttga tatgttcaat caagcatcaa atgaaggtta atttatggac
     6061 gaaaaacagt tacaggctct ggctaacgaa ctggccaaaa acctcaaaac ccctgaagac
     6121 ctcagccagt ttgaccgtct actgaaaaag atcagcgttg aagccgctct caatgcagag
     6181 atgacacacc atcttgggta tgagaaaaat cagtccaaac cgggaactaa ctcccgcaac
     6241 ggttattcca caaagaccgt tatcacaggc gacggtccac tggaactgcg tactccgcgc
     6301 gatcgtgacg gtaccttcga accacaactg gtaaagaaaa atcagaccct tattactggg
     6361 atggataacc agatcttctc gttgtatgcc aaagggatga ccacccgtga gatcgctgct
     6421 gcgttcaaag agctgtatga cgcagatgtt tcaccggcac tgatatcaaa ggtcaccgat
     6481 gccgtgatgg agcaggttgt agaatggcaa aaccgaccgt cggatgctgt ttaccccatt
     6541 gtttatcttg actgtatcgt ccttaaagtt cggcaggaca gtcgcgtcat taacaaatcg
     6601 gtgttcctgg cactgggcat caatatcgaa ggtcagaaag aactgccagg tatgtggctg
     6661 gccgaaaatg aaggggcgaa gttctggctc aatgtgctga ctgaactgaa aaaccgcggt
     6721 ctgaacgata tcctcatcgc ctgtgtggat ggcctgaaag gcttcccggg ggccatcaac
     6781 acagtatatc cgaaggcccg catccagtta tgcatcgtgc atatggtgcg caacagcctg
     6841 cgcttcgtgt catggaagga ctacaaagcc gtcactcgaa acctgaaagc gatttatcag
     6901 gctcccacgg aagaggccgg ccagcaggca caggaagcgt tcgctgcgtc ctgggacagt
     6961 cgctatcctc atataagccg aagctggcag gctaactggc cgaatcttgc cacgttcttc
     7021 gcttatgcga cgggcatccg caaagtgatc tatacgacga atgccatcga gtcgctaaac
     7081 agcgtgatcc gccatgcgat caaaaagcgt aaagtgttcc cgacagacga ctcggtgaaa
     7141 aaagtggtat ggctggcaat ccagtcagcg tcccagaaat ggacgatgcc gttgaaggac
     7201 tggcgtatgg caatgagccg ctttattatc gacttcggtg accgcctggg tggtcacttc
     7261 tgagaaaggg catttacaca gattcctaaa caggctccct ggaaataccc cacagccccg
     7321 aaaaagagcc agtcagttgc tgaactgact ggcattggct cctggggcac tttattgtta
     7381 atcagtcact gatgtttccc cggttatttc aaccctgcgg ttggggcgca gacattcctt
     7441 cagtgcttcg ccctccacat ccgggcagaa caccactggt gcatcatatc cacggcccgt
     7501 tgcatgtatc ctttcagccc tgaaaccccg ggcaataaac catcccctga ctgattccgc
     7561 ccgtttctct gacaggagcc ggttatacgc ctgcccgcca agatggtccg taaatccttc
     7621 tatcttcacc ccgacaacat gacccgcctg aaacttctct gccacacggg aaa
//
LOCUS       DAZGAP010000207         7632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGAP010000207 DAZGAP010000000
VERSION     DAZGAP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.208"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(167..310)
                     /gene="ypaB"
                     /locus_tag="ACHLAM_004465"
     CDS             complement(167..310)
                     /gene="ypaB"
                     /locus_tag="ACHLAM_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO7855898.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     VIS"
     gene            438..2723
                     /gene="nrdA"
                     /locus_tag="ACHLAM_004466"
     CDS             438..2723
                     /gene="nrdA"
                     /locus_tag="ACHLAM_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7855899.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2789..3919
                     /gene="nrdB"
                     /locus_tag="ACHLAM_004467"
     CDS             2789..3919
                     /gene="nrdB"
                     /locus_tag="ACHLAM_004467"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7855900.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            3919..4173
                     /gene="yfaE"
                     /locus_tag="ACHLAM_004468"
     CDS             3919..4173
                     /gene="yfaE"
                     /locus_tag="ACHLAM_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO7855901.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4227..4877)
                     /gene="inaA"
                     /locus_tag="ACHLAM_004469"
     CDS             complement(4227..4877)
                     /gene="inaA"
                     /locus_tag="ACHLAM_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO7855902.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKSEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5080..6156)
                     /gene="glpQ"
                     /locus_tag="ACHLAM_004470"
     CDS             complement(5080..6156)
                     /gene="glpQ"
                     /locus_tag="ACHLAM_004470"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7855903.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6161..7519)
                     /gene="glpT"
                     /locus_tag="ACHLAM_004471"
     CDS             complement(6161..7519)
                     /gene="glpT"
                     /locus_tag="ACHLAM_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO7855904.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
BASE COUNT         1957 a         2072 c         1778 g         1825 t
ORIGIN      
        1 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
       61 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      121 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcaccg
      181 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      241 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      301 agagggccat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      361 tctaagcagc ttccctcact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      421 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      481 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      541 acgtttcgat ttcccaggtc gaactgcgct cccacattca gttttatgac ggtatcaaga
      601 cctctgacat ccacgaaacc attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
      661 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
      721 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
      781 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
      841 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
      901 atctggtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
      961 tggttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1021 gtttttacga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1081 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggc gacagcctgg
     1141 attccatcaa cgccacctcc agcgcgattg ttaaatacgt ttcccagcgt gccgggatcg
     1201 gcatcaacgc cgggcgtatt cgtgcgttgg gtagcccgat tcgcggtggc gaagcattcc
     1261 acaccggctg tattccgttt tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1321 gcggtgtgcg cggcggtgcg gcaacactgt tctacccgat gtggcatctg gaagtggaaa
     1381 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1441 acggggtaca aatcaacaaa ctgatgtata cccgtctgct gaaaggtgaa gatatcaccc
     1501 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1561 ttgaacgcct gtacaccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     1621 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     1681 agaacgtaga ccactgcaac acccacagcc cgtttgatcc ggctattgca ccagtgcgcc
     1741 agtctaacct gtgcctggaa atcgccctac cgaccaaacc actgaacgac gtcaacgacg
     1801 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaacagcc
     1861 tggatgaact ggaagagctg gcaattctgg cagtacgcgc actcgacgcg ctgctggatt
     1921 atcaggatta cccgatcccg gccgccaaac gtggagcgat gggtcgtcgt acgctgggta
     1981 ttggtgtgat caacttcgct tactacctgg cgaagcacgg taaacgctac tccgacggca
     2041 gcgccaacaa cctgacgcat aaaaccttcg aagccattca gtattacctg ctgaaagcgt
     2101 ctaatgagct ggcgaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2161 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2221 tgcattacga ctgggaagca ctgcgtgagt caatcaaaac gcacggcctg cgtaactcca
     2281 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2341 ttgaaccgcc gcgtggttac gtcagcatca aagcgtcgaa agacggtatt ctgcgccagg
     2401 tggtgccgga ctacgagcat ctgcacgacg cctatgaact gctgtgggaa atgccgggta
     2461 acgatggcta cctgcaactg gtgggtatca tgcagaaatt tatcgatcag tcgatctctg
     2521 ctaacaccaa ctacgatccg tcacgcttcc cgtcaggaaa agtgccgatg cagcaactgc
     2581 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     2641 gtgacggtgc tgaagacgca caagacgatc tggtaccgtc aatccaggac gatggctgcg
     2701 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaaa tgccttatcc
     2761 ggcattaaac tcccaacagg acacattcat ggcatatacc accttttcac agacgaaaaa
     2821 tgatcagctg aaagaaccga tgttctttgg tcagccggtt aacgtggctc gctacgatca
     2881 gcaaaaatat gacatcttcg aaaagctgat cgaaaagcag ctctctttct tctggcgtcc
     2941 ggaagaagtt gacgtttctc gtgaccgtat cgattaccag gcactacctg aacacgaaaa
     3001 acacatcttt atcagcaacc tgaaatatca gacgctgctg gactccattc agggtcgcag
     3061 cccgaacgtg gcactgctgc cgctgatctc gatccctgag ctggaaacct gggtcgaaac
     3121 ctgggcgttc tcagaaacca ttcactctcg ttcttatacc catatcattc gtaatatcgt
     3181 taacgatccg tctgttgtgt ttgacgatat cgtcaccaac gagcagatcc agaaacgtgc
     3241 ggaagggatc tccagctatt acgatgagct gatcgaaatg accagctact ggcatctgct
     3301 gggcgaaggt acccacaccg ttaacggtaa aaccgtaacc gttagcctgc gcgagctgaa
     3361 gaaaaaactg tatctctgcc tgatgagcgt taacgcgctg gaagcgatcc gcttctacgt
     3421 cagctttgct tgttcctttg cctttgcaga acgcgaactg atggaaggta acgccaaaat
     3481 tattcgcctg attgctcgcg atgaagccct gcacctgacc ggtactcagc atatgctgaa
     3541 tctgctgcgc agcggcgcgg atgatccgga gatggcggaa attgccgaag aatgtaagca
     3601 ggagtgctat gacctgtttg tccaggcggc acagcaagag aaagactggg cggattatct
     3661 gttccgtgac ggttcgatga ttggtctgaa taaagacatt ctctgccagt acgttgaata
     3721 catcaccaat atccgcatgc aggcagtcgg tttggatctg ccgttccaga cacgctccaa
     3781 cccgatcccg tggatcaata cctggctggt gtctgataac gtgcaggttg ctccgcagga
     3841 agtggaagtc agttcttatc tggtcgggca gattgactcg gaagtggaca ccgacgattt
     3901 gagtaacttc cagctctgat ggctcgcgtt accctgcgca tcactggcac acaactgctg
     3961 tgccaggatg aacacccttc ccttctggcg gcgctggaat cccacaatgt ggcggttgag
     4021 taccagtgcc gcgaaggtta ctgcggttcc tgtcgcacgc gccttgttgc aggccaggtg
     4081 gactggattg ccgaaccgtt agcctttatt cagccggggg aaattttgcc ctgttgttgc
     4141 cgggcaaaag gcgatattga aatcgagttg tgatcgggta ctagcgccag atacgacgct
     4201 tacgcgtatt atctggccta caacgattac atggcgtagt aatacgcttt cacctgctcc
     4261 cagtccgctt tagggatcgg ctccagatat ttttccagct ggcggaagtc atgattaatc
     4321 gctttatcgc gacgcaagcg gcgacgactt ttctccagat caagaaaacc agcttctgac
     4381 ttaccttctg ttttcacata gatatggcga acatagcaac agccatgctg acgattaacg
     4441 ctatgcattt tcttaaacgc cagcgccact gctttcaaca tggcttgccg tacttcgtca
     4501 gaatagggcg ttactgcatg ctgggcatac cagtcagcaa tgctgatgaa ccccgccata
     4561 tcttcagtca ccagcaacgc gcgccattcc ccctcaattt tcaccgcttc accaaaaacg
     4621 atcttcggca cgatgacacc ggcccgttcc agttctttaa ttaccgcaac ctcacggaca
     4681 atcgttggtc ggccgaacgg ataacgtacg gaatgaaaca gatgatgcgt catgcgcttt
     4741 acatatagct ttttgccgtt gcgctcgacg cattgcaccc cgctcatacc attacggcga
     4801 tagttaggct cttcaaccca gtcgccctct gttgcccacc agtgattaaa ttcgtcgtgc
     4861 tttgctgaaa ctgccatacc ctatcgcctg tcgtttttta ttaacgacaa tgactataga
     4921 tgtttagctg aggaaaatct taataatacg tgttgtattg acgagtatct tatgccggga
     4981 acaagtcatc tcgctcaaaa taattgcaat taaggataac ccacagactt taatgaagag
     5041 tggtatcgga tagcacataa gtgccatccg gtagttaatt tactctttat taaggaattt
     5101 tactgcctta tcagggaaat cagtaaatag cccatttaca cccgctttgt tatacagagc
     5161 atcatataac tgattcacat cagtagtgta ttcaggcagt ttatctgacc gcacggtata
     5221 aggatgcact accagcttgt tctgctgagc atcttgcacc atgccagtga gtttgatatt
     5281 acctggctgc gatgtctcct caatcaacat atgataatcc ggaccaatac catcagcata
     5341 ttccgccacc tgtttcatgg cccccggctt aaacatccag tcgtagttgt aattaaccca
     5401 gcttccgtcc ggctgtttct gctgagtttc gttccagtcg gtataggcaa tcagctgtac
     5461 cagattgagg tccatgccca ttttaggttc cagctcattc ttaatacgct tcagttcatc
     5521 ggcatcaaaa cattgcaaat aaactttgtc gtccttaccg ctgtaaccat atttcttcag
     5581 cacttccagc gtttttgccg caatatcctt cccttcctga tgatggaacc acggcgcttt
     5641 aatttctgga tagataccga tatttttccc ggtagagtga tttaacccct gaacaaattc
     5701 aatctcttcc tcgaaagtgt gtacccggaa gtcagattta cccatcggga aacgccccgg
     5761 ataagtctgt actttcttac cattttcaat atcgaaacct tcggtaaatt tcagtgactt
     5821 aatttcatcg agcgtgaaat ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg
     5881 gaaacgatcg gcaacatcag taacacgatc gagataatgg tcatgcagga caaccagatg
     5941 gtcgtctttg gtcatcacca aatcttgttc cagataatcc gctccctgcg catacgccat
     6001 cgcttttgct ggcagcgtat gctccggcaa atatccactg gcaccgcgat gggcgatgac
     6061 tattttttcg ttgctgtccg ccgccattgc actgcttccc atgatcattc cactcatcat
     6121 gatcgccatg ctaaggtttt tcagcgtcaa tttcatgcca ttagcctccg ttgcgttttt
     6181 gcagtaattc ctgatggcga cgtttttcgc caatcatcac aacaatcaac aagataaccg
     6241 ccagaatgct gccgccaatc attaccataa agccgccatc ccagccgaag aagtccactg
     6301 tgtagccaac aatcgcgctc gccgccaccg aaccgcccag gtaaccaaac agcccggtaa
     6361 agcccgctgc cgtacctgcc gcttttttcg gtgccagttc cagcgcatgc agaccgatca
     6421 gcatcacagg gccgtagatc aggaagccga taacaatcat acaaatcata tctacggttg
     6481 ggttacctgc cgggttcatc cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga
     6541 agacgccggt ggccccacgg ttgccacgga agactttatc cgacatccag ccgcacagca
     6601 gggtgcccgg aatgcccgca tactcataga agaagtaggc ccaggaggat ttatccagcg
     6661 cgaagtgttt cacctctttc agataagtcg gtgaccagtc gaggatgccg taacgcagca
     6721 gataaacgaa cacgttggcg atagcgatgt accacagcag tttgttcggc agcacgtact
     6781 gcatgaagat ttgcttcgcc gtcagctcct gttccgcttt ttcgttatag tcgtccggat
     6841 aatcattttt gtactcttcg atcggcggca ggccacagga ttgcggggta tcgcgcatca
     6901 tcgcaaaggc gaacagtgcc accagaatgg cgcagaaagc gggcatatag agcgccgcgt
     6961 gccagtcatt gaaccaggcc atccccagca ggaacagcag cggcggaata ccaccaccga
     7021 cgttatgcgc acagttccac actgacacaa tgccgccacg ttctttctgc gaccaccagt
     7081 gcaccatagt acgaccgcac ggcggccatc ccatcccctg gaaccaaccg cagaggaaca
     7141 gcagcacaaa catcaccgca atgctcgacg tcgcccacgg cacaaagccc ataaaaagca
     7201 tcaccgccgc cgccagaatc aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg
     7261 acaccgagcc catgatgaat ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa
     7321 aacctaaatc accgcgtgag aatccctgct cgaccagata aggcatagcg agggcaaagt
     7381 tcttacgaac caaatagtaa gcagcataac caaagaatat ccccaggaaa atttgccagc
     7441 gcaatcgacg ataagtcgga tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg
     7501 gtttaaaaat actcaacatt gatagcctcc gtggcccgtg gtcttattta tgattaacag
     7561 cctgattcag tgggagaacc tgccgtttct tgagttgccg cgatgttaag aaaacattca
     7621 taaattaaat gt
//
LOCUS       DAZGAP010000208         7610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGAP010000208 DAZGAP010000000
VERSION     DAZGAP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.209"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHLAM_004472"
     CDS             <1..285
                     /locus_tag="ACHLAM_004472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855905.1"
                     /translation="VLITACNHWVLNSRDIIDIFNLSIKHDDYGEINRMYYWLPCEIR
                     GRLMYDGIEWSFVINAAATAMWMSNDKEIYWGCNKKACDNFFLLPYDGMR"
     gene            391..1605
                     /locus_tag="ACHLAM_004473"
     CDS             391..1605
                     /locus_tag="ACHLAM_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855906.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVGSVRWLVFVTQHIRADAWNQRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARHQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTVELNREQRASLLSS
                     NQPFTQEALMYELDAFLGCSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQNGHYSWAT
                     MVSPFVSPQEAS"
     gene            1662..4988
                     /locus_tag="ACHLAM_004474"
     CDS             1662..4988
                     /locus_tag="ACHLAM_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO7855907.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALVQETSIQTARALDIRLPLSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADDLRY
                     AGVPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDMLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGSLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            4981..6591
                     /gene="tssA"
                     /locus_tag="ACHLAM_004475"
     CDS             4981..6591
                     /gene="tssA"
                     /locus_tag="ACHLAM_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7855908.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGRMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADELTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            6597..>7610
                     /locus_tag="ACHLAM_004476"
     CDS             6597..>7610
                     /locus_tag="ACHLAM_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7855909.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDYPLKNAAKEEEWVDVKVDRNNKVVDITWRLSVKDSGTKG
                     SNPELSPIPYSDLVSLTKNGVEFYWSRNGSRGGGIGGNIVTAIGVFKVNVKAEINITP
                     SMRT"
BASE COUNT         1763 a         2021 c         2265 g         1561 t
ORIGIN      
        1 gtgcttatta ctgcgtgtaa tcattgggtg cttaattcta gagatattat agacatattc
       61 aatctaagta taaagcatga tgattatggc gaaataaaca gaatgtatta ttggttacct
      121 tgtgaaatta gagggagact aatgtatgac ggaatagaat ggagttttgt tatcaatgct
      181 gctgcaacag caatgtggat gagcaatgat aaagagattt actggggatg caataaaaaa
      241 gcatgcgata atttcttttt attaccatat gatgggatga ggtaaatcac agttaataaa
      301 gcaaaaacat tatggacaag tgaattaatc agatagttca agcaccttga gattcattgt
      361 attcatattt cagaataacg gattattaca atgcccatcc gacttaatac cattcccgac
      421 gcagaaccct atcccgcacc gccagcccgc attaaatggc tgtcagcctt attactgatg
      481 ctgtctgttg gtgtggcaat aacctcatta ttcgccagcg atgaactggc aaaaaacggc
      541 ccatatttct gggggctggc ctgcggcgtg cctgctttta tctggtcact ggtcggttcg
      601 gtgcgctggc tggtatttgt cactcaacat attcgtgccg atgcctggaa tcaacgtcgc
      661 gaagaagtca ttttgcagga aacacggcgg ggacgacggg cgttgcagat actcagtttt
      721 tccgtacaaa ccgcgctgaa tggtgattcc gtcgcagaaa ctacaacagc atttctggcg
      781 cgtcatcagg tgttgaatac ttacgctgat ttgcgcggag aagataccgt gcgtcgcagt
      841 gtcattcccg tattaaccaa taaaccggtt accgggcgtt tatccgaaat tatttcccgc
      901 ctttttattg atatcagacc gcaattgttt ctgctgcccc ctgattttca tatcaacgta
      961 ctactggaaa ttgatgcgcc gctttcaggg gcctccgtga gaactatctg gcaggacgaa
     1021 tggcaaaaag ccggactccc cgaagcccgt ctgttttctg ccccggagcc ggggctggcc
     1081 gccgtggacg actggctgga caactttatt caggaaaagg ccgtactgct ggtgatttcc
     1141 gtgcgtctgg aaccgaagaa cccggaacgg acggcagaat ccgccaccgc gctgttgctg
     1201 gcaaaccgac tgacgcaaac cgcgctgacg ccgctggccc tgctgcatcg tcctgaacgt
     1261 atcacagata cggagatgat ggcgtcaggc attgcacagg cgctggactg gatgccggtg
     1321 cagccagatg ccatatccgg catgtggaca gtcgaactga acagggaaca gcgcgcctcg
     1381 ctgctgtcat ctaaccagcc gtttactcag gaagccctga tgtacgagct ggatgccttt
     1441 ctggggtgct ccggaccggc agcgccgtgg ctgtccgtcg ctgcggcaac gctggcggca
     1501 atacaatcac aacaccccca actgacgctc agcggcgttc agaacgggca ttattcatgg
     1561 gcgaccatgg tgtcgccatt cgtttcccct caggaggcat catagtggtt ccgggattaa
     1621 accgtctccg gccccgttcg gtttcagggc ctgccatcgt catgaccgtg gccgttgtgg
     1681 ttgcgctttg cacgtttatt caccgtaaac cggcattgct gggcgttgaa ccgggtagtt
     1741 cacagcaaat gtactggatg gctggcgtga ttgccgcagg tgtggtcttt ctgattatca
     1801 tgggggttta tcatctgctg aaccgccacg ctgccgtaca gagctttgaa ctccacagac
     1861 agcttgccgc cggagatgac tatcgggcgg tgacgacaaa gaaagccgac agccgtcatc
     1921 cggcaatggt ggaactcgcc gccttcctgc gcgaccgcta cggcccattc tggcggcgta
     1981 aaatccgcct gctgctggtg accggcgagc ctgaacaggc agaggccatc gcgccggggc
     2041 tgaccgggca acactggctg gagggcgacc acacggtgct gatatatggc ggcaggccat
     2101 ctacagagcc tgatgtcgcg ctactgaccg ccttaaaaaa actgcgccgc agccgtccgc
     2161 tggacggcat catctggccg ctgacagaag aacagagccg ccagacggcg caactcgaca
     2221 aaggctggcg cgaactgata aacggcggta agcgactcgg ttttcaggct ccactctatt
     2281 tgtggcaggt ctgtgacaac ggtgattatc agaccggacg ccccctgcaa agcgtcggct
     2341 gcctgctgtc ggaacgctgt accccggaac aactggctgc aatgctggaa gcacaaaccc
     2401 tgccgctgac ggaacagggc atgtcgcagc tgcttaccga taaccgccat gatttcctgc
     2461 tgcgtctggc ccataccctt gcagagcggg gtatcgctca ctggcaaagt atcctgaaac
     2521 cgctgctggc aggcggcgca ttttcctccc tgcgcctgcg tggcctgatg ttcagcccgc
     2581 cgcttaccgc cgtaccggaa gccgcacctc atgcgtggct gccgtcaccg gtctgggcgg
     2641 gcgtgaccgg cgataacgcg cggggtcgca cggtgggctt tccgtggctg cgtatcacgc
     2701 tgatgtctgc tgtctgcgta ctggcgatat ggggggccgg aatgacgacc tcgttcttcg
     2761 ccaaccgcgc acttgttcag gaaaccagca tccagacggc gcgtgcactc gatatccgtc
     2821 tgccgctgtc tgaacaactg gtggcgctgc ataccctgca gggtgaactg gagcgcctgc
     2881 agtaccgtat ccgggagggt gcgccgtggt atcagcgttt tggccttgaa cgtaaccagc
     2941 aactgctcgc cgccgccttt cccggctatg cgcaggcggc aaaccggctg gtgcgcgacg
     3001 tggccgttga ccatctgcaa cagcaactga atgcctttgt cgcgctgccg cccaatagtc
     3061 cgcagcgtac cgccaccggc gaacagcgct ataagcagct taaggcattg ctgatgactt
     3121 cccgcccgga aaaggccgac gctgcctttt tcagtaccac gctgatggca gatgacctgc
     3181 gctatgcagg tgtaccggaa ggtgtgcgtc agagcgtgtt gccgtcactg ctgaccttct
     3241 ggatggcgaa tctgccggaa cacccacagt ggaaaacttc gccgccaccg gaactgaccg
     3301 gcgcagtgcg caaaatcctg ctgcgccaga ttggtgtgcg caatgccgaa aacactctct
     3361 accagaacgt actgcaacag gtgtcccgca actacgccga tatgacgctg gcggacatga
     3421 ccggggatac gctcaccgaa tctcttttca gtacggagca gacggtgccg gggatgttta
     3481 cccgtcaggc gtgggaagga caggtcaggg aagccatcga gcaggtggtg acggcgcggc
     3541 gcgaggaaat cgactgggta ctcagcgacc gccagcagga tacctctgcg gatatctcgc
     3601 cggatatgct gcgtaaccgt ctcacttcac gctactttac cgactttgcc ggaagctggc
     3661 tggcgtttct caacagcatt cgctggaaaa aggaagactc gctctccggc attctcgacc
     3721 agttgacgct gatggccgat gcccgccagt caccgctgat tgcgctgaca gacaccctcg
     3781 cgtggcaggc ggcggctggc agggaaaacc gtggcctgtc agactcgctg gcgaaatcgg
     3841 cacaggaact gtttaacggc aaggagaaaa cgccgcagca atcccgtgaa ggcgacgtgc
     3901 ctgtcgggcc gctggataaa accttcgcgc cgctgctgcg gttgcttggc gataaggcag
     3961 gaggcggcga cagccagctg agcctgcaga cctacctcac ccgagtcacc cgcgtgcgcc
     4021 tcaaactgca acaggtgacc aacgcccctg acccgcagga gatgacccag caactggcgc
     4081 agacggtctt acaaggcaaa accgttgacc ttaccgacac ccgcgactat ggcagcctga
     4141 tagccgccag tctgggtgaa gagtggagtg gcttcggtca ggcgctgttc gtccgcccgg
     4201 tggagcagtc atggcggcag gtgctgacgc ctgcggcaga cagcctgaac cgccagtggc
     4261 agcgggcgat tgtcagccac tgggatcagg acttcgctgg tcgctacccg ttcaaagcct
     4321 cacaaaacga tgcctccctc cccctgctgg cgcagtacct gcgcgatgac gggcgcatca
     4381 accagtttat cgccgccaat ctttccggcg tactgaaacg tgaaggccgc tactgggtgg
     4441 ctgacgccat gaacacgcag gggctgacag tcaatccgga ctttattcgc gccctgaacc
     4501 gcctgcgcga tgtggccgat accgcctttg ccagcggcga tgcagggata cattttgaac
     4561 tgcgggcaaa accggcgcgc gacgtgatga agacgcatct ggtgattgac gggcaggagc
     4621 tggaatattt caaccagaag gaacgctggc agcgttttaa ctggccggat gaacagtggc
     4681 aacccggcgc atcgctgagc tggaccagta cgcaggcgat ggaacgcata ctggcggatt
     4741 atcggggaag ctggagcctt attcgcctgc tggaacaggc gcaggtgaca ccggtggaca
     4801 gcagtacctt taaagtggtg tggaaagcgc aggacggcct gccgctgaat tacctgctgc
     4861 gggttgaaca gggtaaaggg ccgctggcgc tgctggaact gaaaaacttc cgcctgccgg
     4921 gacaggtgtt cctgaccggc aggagtatga aggatgcgga agaatacggg gaagacgccg
     4981 atgagtgaac tgttgcaaaa gctgacccgt agctgttttg ccgaccggga cgcactggac
     5041 tcggcgcgaa cacaggcggc gctgtggcag acatggctgc tgccggtgac ggcagatacg
     5101 ccggtcgggg aagaccccgg ctaccacgac gatttcctgc gtatccgcga cgagatgaac
     5161 aaactttccg gcgcggatac ggaccttatc tgccagcttg ccgaatccct gctgctgacg
     5221 caggcaaaag acgtgcgcat cgccacttac tatatctggg cgcgactgca ccgcgatggc
     5281 gaacgcggac tggctgaggg gctggcgctg ttagccggac tggtggaacg cttcggtgcg
     5341 caactgctgc cctcgcgccc ggcaagccga aaaatggcgc tggagtggct ggcaggggaa
     5401 aagatgctcg acagccttgc ccgctacccg gaagtggcaa aagaggattt tgcgaacatt
     5461 gttgccgctc ttaatcaact gacggtttcc ttcactgcat ggcccgaaga ccagcattcc
     5521 ccctccctga tgccgctgat taacgcgctg gaatcacgtc tggcgcagtc gggtaggatg
     5581 aacgcggtgg tgccgcagaa cagcagcggc gttccggcac cgtcttcccc ggtggatgcg
     5641 ccacaggtac agaccatcac ttccgggcgc gacctgctcg accaggcgaa agtgctggcc
     5701 cgttatctga acgaacagcc gcagggctgg ctttcagcac accggctgat gaaaaccctg
     5761 cgctgggaca cggtgcatga actgccgccg gatgtggacg gaaaaacccg gctggccccg
     5821 ccgcgcacag aatcacgcaa ccagcttaag cggctgtatg ctcagcagaa ctggacggaa
     5881 ctgctggagc aggccgacct gatgttcagc accggcgtca gccatttctg gctggacatt
     5941 cagtggtatc tgcatcaggc gctgactaag gcgggcgcac cgtgggacag gtggacagcg
     6001 gttatccgtc aggacctggc gttactactg gaacgcctgc cggggctgga aaatctggca
     6061 tggaacgacg gcacgccgtt cgccgatgag ttgacgcgca actggatagc gcagcaggtc
     6121 atgatgcggg aagacggcgc atggcttgcg gggaaagccg ctgtaccgac agacgacgcc
     6181 acaaacgacg tactggcgct ggagccggaa gcgctggaga tggcagacag tcagggcgtg
     6241 gaagcggcgc tgggctggat acagacccgt ccgggcatca ccacggcacg ccagcgcctg
     6301 ttattgcgac tgctgatggc gcgggtggcg gaacagtacg gtaaaaacga gatggcgctc
     6361 ctgctgctgg aagaactgga tatcgccgca caggggctga cgctgacgca gtgggagccg
     6421 gacctgctgt ttgaggtaaa agcccgacag ctgaaactgc tgcgcctgcg ggcgcaccgc
     6481 tatgcggata aagccctgct gaaccgaaaa atggaaatcc tgctgggaac gctggtgaca
     6541 atagatccgg tacgggcagc ggtattgtgt gatacgcaac ataaggactg atggcaatgg
     6601 gaacaggcta ctttctggtc aggggggaca aaaccacctg cggcggcaaa attattgaag
     6661 gcgcggacga ccatacgatc atgggtatcc cgcaggcgcg ggatatggac cgggtcacct
     6721 gcggcagata tccgggaatg tttattattg tgggcggcgt tcctgaaaca gatattcacg
     6781 gcaggctgat ggcggggacg ctggacagtc agagcagttg cccgtgtaag gcacggttta
     6841 ttgcgtcaat gatggatgat acgtatgaga cggatgacgg ggaaagtgaa gcggaacaac
     6901 atgcgcagtc ggcgaaaaag gatctgactt ctggttctga ttcatcctct gatgatgtaa
     6961 aactggatta cagaattaaa ttatcaggta ataagatact gacgccactg aatatacctg
     7021 attataaaga aatgattagc ggtggtagta cgaagaatac agaaaaaata gattttacca
     7081 tcacaaataa aggcgatgag gctgaagcgc tttctctgga agtgcttgat ggtaatgaag
     7141 tgatttatag tgaacggcag accggtaaat attgtgataa gggagagcat gcctggcaat
     7201 gggatggcta cagtaatcaa ggcatactgg atacaactaa gataaaaagc aaatcactac
     7261 tcgtaagact gattgcttta tgtggtgata tgatgataaa agttgattat ccattgaaaa
     7321 atgctgcgaa agaagaagag tgggttgatg taaaagttga cagaaataat aaagtggtcg
     7381 atataacatg gagattatct gttaaggata gcggtacaaa aggcagtaac cctgagttat
     7441 cgccaatacc ttatagcgat ttagttagtc ttactaaaaa tggcgtggag ttttactggt
     7501 cacgaaatgg aagtcgcggc gggggaattg gtggtaatat cgttacagcg attggggttt
     7561 ttaaagtaaa tgtaaaggct gagataaata ttactccatc tatgcgaact
//
LOCUS       DAZGAP010000209         7609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGAP010000209 DAZGAP010000000
VERSION     DAZGAP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.210"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..1653
                     /locus_tag="ACHLAM_004477"
     CDS             43..1653
                     /locus_tag="ACHLAM_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO7855910.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(1659..2471)
                     /gene="ydhT"
                     /locus_tag="ACHLAM_004478"
     CDS             complement(1659..2471)
                     /gene="ydhT"
                     /locus_tag="ACHLAM_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO7855911.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(2475..3260)
                     /gene="phsC"
                     /locus_tag="ACHLAM_004479"
     CDS             complement(2475..3260)
                     /gene="phsC"
                     /locus_tag="ACHLAM_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO7855912.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(3257..3925)
                     /locus_tag="ACHLAM_004480"
     CDS             complement(3257..3925)
                     /locus_tag="ACHLAM_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7855913.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3989..4627)
                     /locus_tag="ACHLAM_004481"
     CDS             complement(3989..4627)
                     /locus_tag="ACHLAM_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO7855914.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(4640..6742)
                     /locus_tag="ACHLAM_004482"
     CDS             complement(4640..6742)
                     /locus_tag="ACHLAM_004482"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO7855915.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(6763..7389)
                     /locus_tag="ACHLAM_004483"
     CDS             complement(6763..7389)
                     /locus_tag="ACHLAM_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO7855916.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTHRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         1984 a         1979 c         1721 g         1925 t
ORIGIN      
        1 tgataacact aaattcgaaa gaatgcgtac aggtaagtaa caatgaaaaa aattgctatt
       61 gtgggtgccg ggcctacggg gatctacacc ttattctcgc ttctacagca acaaactcca
      121 ctttctattt ctatcttcga gcaggctgat gaagccggtg tcgggatgcc atacagtgat
      181 gaggaaaact caaaaatgat gctggcaaat attgccagta ttgaaatacc gccgatttat
      241 tgtacgtatc tcgaatggct acaaaagcaa gaagccagcc atctccagcg ttatggcgtt
      301 aaaaaagaaa ccttgcacga tcgtcagttt ttaccgcgaa ttctgctggg cgaatatttc
      361 cgcgatcaat ttttacgatt agtagaccag gcacgacagc aaaaatttgc agtggctgtt
      421 tatgaatcat gccaggttac cgatctgcaa attacaaatg ctggcgtcat gctcgctaca
      481 aatcaggatt tacccagcga gacgtttgat ttagcggtga tcgccacggg tcacgtctgg
      541 cctgatgaag aagaagcaac ccgaacgtat tttcccagcc cgtggtcagg cctgatggaa
      601 gcaaaggtcg atgcgtgtaa cgtgggtatt atgggaacat ccttgagcgg actggatgcg
      661 gcaatggcag tggctattca gcatggttcg ttcattgaag atgataaaca acacgtcgtt
      721 tttcaccgcg ataacgcaag tgaaaagcta aatattacgt tgatgtcgcg tacgggtatt
      781 ttacccgaag ccgatttcta ttgccctatt ccctacgagc ccttacacat cgtcaccgat
      841 caagcattaa atgctgagat tcaaaaaggc gaagtgggcc ttttggatcg ggtatttaga
      901 ttgatagtag aggaaatcaa atttgctgat ccagactgga gccaacgcat agccttagaa
      961 agcctgaatg tcgattcctt tgctcaagcc tggtttgccg agcgcaaaca acgcgaccca
     1021 ttcgactggg cagaaaaaaa tctccaggaa gtcgaacgca ataaacgaga aaaacatact
     1081 gttccctggc gttatgtcat tctgcgcctg catgaagccg tacaggaaat tgttcctcat
     1141 ctgaatgaac acgaccataa acggttcagt aaaggccttg cccgggtttt catcgataat
     1201 tatgcggcaa tcccttcaga gtctattcgt cgactactgg ccttacgtga agcgggaatc
     1261 attcatattc tcgccctcgg tgaagactac gaaatggaaa ttaatgagtc gcgcaccgtc
     1321 ctgaaaacgg aagacaatag ctactcgttt gacgttttta ttgatgcccg tgggcagcgt
     1381 ccgcttaaag tgaaagatat tcctttccct ggactacgcg aacaattaca gaaaacaggg
     1441 gatgaaatcc ctgatgttgg cgaagattat acgctacagc aacccgaaga aattcgtggg
     1501 cgcgtagcgt tcggcgcgtt gccctggttg atgcacgacc agccattcgt acagggactt
     1561 acggcatgtg cagaaattgg tgaggcgata gctcaggcgg tcgtaaagcc tgcatcccgt
     1621 gctcgtcggc gtctttcgtt taatcaaatg taaagctatc agcactcaac aaactgatgc
     1681 tgttcattaa ggcgatagcg gactccggct cgaatattgt tttcaccttc aatggcgacg
     1741 gcgaagcgaa ctctttcgcc gtcatgatat gccagtgcgg cgcttccccc tggcccgaga
     1801 ataattgagt cgaccacgcc cgtgctcact attgttgaat cttcaccgct ggcaatgatg
     1861 tgtacattgt cgccgctgtt ggcaattctg gcgtttgcgc caaagctggc gatttgcacc
     1921 aggtcaccat tactggcgac atgacatcgt tcacccaatg tacaaacgcg tactcgcatt
     1981 ccggtattcg caatccgact gctatctcca gcgctgctta tccgcgaaga attaccggtt
     2041 acagcaaccc gtgcgcgttc accggagctg ccaatgtgac tgttaaagcc gacacttcca
     2101 atccgcacgc tataacccat actggcaatt tgcgcggcat acccagcggt tgcgattctg
     2161 gcattatcct cacaacagcc gatgcgagca tgatcgtcgc tgcgggcaaa ctgactgcaa
     2221 aatgctctct ctccgggatc tgtggccagt ttttgcatag aagaaacttc ctgatgcgcg
     2281 aagttttcat ccgccagcca ttttttccac gcatactcaa ccagactctc cgcccagtcc
     2341 gtatatcctt cctcaatcag tgcatgatga atatcggcat aactaccgcc gcgtgggaaa
     2401 tggcgcagaa accagcgata catcaccgct ccgatacgcc attctcgcag gtctgctcgt
     2461 gtaatgatca ttcgttagtg ccggtgatag ccatcgacca tgcttttaaa ggtttcgtgt
     2521 ggcgtacgcc ccgtggtgca aagataaaga tgaccgaaga taaaaaagag gcttataaat
     2581 gccagagcaa aatgcgcctg caataaccag tatcttacgc caggaaacat atctcccacg
     2641 gcttgcggat agagacacag cagccccgtc aatagtaaca atggcagcaa tccatacatg
     2701 acaccaacat aggcgacctg ctgtaaggga ttaaatttag actgggttgt tgccgggaaa
     2761 ggatgttctt ccccctgcat aatgccaaac aaatagaatc gcgtttgttt tgccgctcgc
     2821 tccagccacc cctgacgacg aatgcgatag tggtgaccat tacccccaac ggcattgatc
     2881 agaacaaagc cgagccagca tgccagtaac aaaaatccgc aaacttcatg caccgcaacc
     2941 agacttttaa ccgtagttgc gcccaccagc gcaaagtgat ttatcagccc acttgccagc
     3001 aacaatacaa agagtaacgc attcgaccag tgccataatc tgaccgcttt gctgtaaaga
     3061 taaaccttct caccatggtc gttggctgat ttcttcacgc cacgagcacg aagcaaggca
     3121 tgcagcccca gcaccaacca catccctacc agcaatagtc cggcaatgat caaccacacc
     3181 ggccaaaatt ggggagtgaa cagtggcaca tagttcgcca actggctctg aaactgttca
     3241 gcatgttgcg acgggttcat acattttcct ttttaatcaa atgttgaccg aaccggcgat
     3301 acaggtgcgg tttcccggca ccaggtagct gatattgata gtatttattt tcctgcaacc
     3361 acgcctgaat ttctgggcta tcttcgcgac caaagatcaa tgcgtgttcc ggacaggcgc
     3421 tgacgcaaat gggcggaaag ccttttgcca gtcgggactc agcgcagaaa tcacatttat
     3481 ctgccacttt agtgacaggg ttaaggtagc gaacctggta aggacaagcg ccaatgcaat
     3541 aactgcagcc gatgcactgc gatttttcta cccgcacgat cccctgctca tcacgccacg
     3601 acgcccctgt cgggcaaacg tcgatgcaag gagcatcttc acagtgctgg catgactggc
     3661 gaaaaaagtg atactgcgtt tcgttgtcgt tatcggtgac tggaatgtgt gctatcgata
     3721 aacggcttcc ctgagcgggg acgtggttag ttttacggca tgcgcgggcg caaatattac
     3781 agccattgca ccgtgactcg tcatgaatca tggcataccg aactttcttt tcttgcatcg
     3841 tgttcgctaa cagagaagac gcagaaccag taaaaaagat tactgttccc atccccagaa
     3901 caaatttgcg tcgagtgaag gacatctgat tatcccctaa tgcagtggct gctactgtcc
     3961 accctatcca tgacatctcc ctgaaatttc aggggttctg aaactgctct acctcgaacc
     4021 attcgcataa gccatacgcc ttgccatagg tcataaatcg ctcgctaaac aagtacaaca
     4081 ccgccgtcga tgactccacc tgccgtatat cagcatattc aggtgcaata tccatcgcgg
     4141 cgatcgcggc ttcgatctgc gcttcctgga agtaatatgg cgcttgcatc agcatggcga
     4201 ctttgtaagg acgaggatac gtctggcact caaaacgaac ggcgtgtgca atggcgcgac
     4261 aaatgtcctg ctccacaact tgcaaagaca tcgccgcgta gttttcactc atggcctgcg
     4321 ttgagtaata gtaatcatcc tggcttcccg tcatacacgc gatatccgcg tggtctccgt
     4381 tctgcttaat gttttccagc aaaactgaca gttcctcttc tgcaaccgaa tagggcggcg
     4441 agagaaacac ggcgcgcgcc accagctgcc cagccgcaga atgctgacga atgaactctg
     4501 ccaggatttc agcaggttct ggttcggtta ccgtatcaac ggggtgacgc atcccctgca
     4561 accattgccg cttcgcctca tcaacttcgc tgactttcgc taacggcaat tcatcctgat
     4621 ggttcatctc acccattcct tacgcaggca gtagattgtg tgctgccaga tcggcggcaa
     4681 tatcttccag ccccagacgc tgcaatgttt tgcgggtcgg acaaccaagc tgtgggtccc
     4741 agcccatttc tttgtagaac atcgtgagcg aagcatgcat atcgtcacgg tccattttgt
     4801 ctgtaccttc agtaaagacc gggatctgcg gatccttgtc gaatacccag gaacagataa
     4861 gatcgtgttc attacgcata tctttagttt gcatcagttt taccgtgtag gcgcgatgca
     4921 acgtaaagat acgctctgca gctaaatcta atttttcctg agtcatatct tcgccagtga
     4981 tcgctttgaa gaatttggct tcaagcgcca gatcgccccg ataattacgg cttttcagcg
     5041 gcgaaacggt cattggccag acccagttgc acagggtgac ggcgttatgc aaacagaccc
     5101 gcaacagcga ccacttagca tatttaattt ttgcgtcatt gattggcgtg tagtttttgg
     5161 tttcatcgta agcatcttca gaaccaaaaa gttctttcgc cacttcacgt tgcagtttca
     5221 atggcaagcc ggaaccaata aagttgatat gggtatgcgt catgcagtca cggttgaaca
     5281 tacagttaac aatggagccg acctgcgctg acgcttcatt ggcgtggtga actggatagc
     5341 caaacggcga ccagagttta tttttcgcgt agccccagta ctcttcaccc aaattccagc
     5401 gttctgcgat ggcatatgaa ccatcagcca ggtgactcag ctcacccacg cgatgcgcca
     5461 gacggtagta aaaatcttta atgaagttaa cgtcacccgc ttccagttga tcccagcgaa
     5521 tttccgcata ctcttcagca ggcagaacac gcttgaacac ccctttgctg taacaatagg
     5581 taaagtcgcg atgaagctga ccatagttac accataggcc gtagtcatcg aacagattca
     5641 gaccaaccag gttaccaatc acacgaccat catctttatc ttcaaaatct ttcggaccgt
     5701 tcgggaagat ggtggtatgg acaaagtttg ctacacaagt gttaccacct gtgctgggca
     5761 cgccaaactc tttcacccga ggaatattca tttgggtcat acaacggatc gggcaagagt
     5821 ggcagccact catttttact gtgtatttct ctgccgccgg acctaagtca aaaacggatt
     5881 tataggtacg aaagccgacc gtgttctgat tgcctggcgg aatctcaccc gtttcaatcg
     5941 gaccaccttc agccgcgccc caaaacaacc ctttacgtgc agtccaacgt gacttgggat
     6001 ctgaatactc tgcccacgat tgcggcgtac ttggcacgac atggttgtta ttcgcaccga
     6061 taagttcagt catcatgtaa tcgttgagac gcttcatctc ctgacgatcg gcaatgttga
     6121 cccctttcgt cccttcaacc gcaatcgctt tcaggttttt cgaacccatt attgcgccag
     6181 ttcccgcacc accgctgtgg ttacggctat tcaacatgcc agagagagga acaaggtttt
     6241 ccccagcctg accaatagcc gccacacagg tttccggact ggtcaagcga caaatttctt
     6301 ccgtcgttgc gcgcgtccct tttccccata agaaatcggc tttttcaagg ctaactttgt
     6361 catctttaat cttcagccat accggtgatt tcgctttccc ttcgataata atgacgtcgt
     6421 atccagcgaa tttcatttgc gctgcaaaaa agccccccat atgggcatcg acgactaaat
     6481 ttcctttggt aaaagtagaa agtgaggtga tatttacgcg agaactacag ggggcaccag
     6541 atccagttaa tgggccggta gcaaaaacta atttattcgc ttcatcgaaa ggtttcgtgc
     6601 ctggcggtac ttcgtcatac ataattttgt agccgaagcc catgccaccg acaaaacttt
     6661 taaacttact ggaatcttcg agggtaatat ttcctgtcgt gagattgact cttaatatat
     6721 tacctgtcca accgttagcc atgatttttt cctttgcaag attcacacag taatatcttt
     6781 ccactcgata atttttaacg cccctgttgg gcaggcgttt gcgcattcac cgcataacac
     6841 acatttcgag gattttttac tttcggtatt tacggtggcc atcatccacg ggcatgccgt
     6901 agtacaggcg ctacagccaa tacaacgttt atgatcgacg gtaatacagc cttctttctg
     6961 ctgccaggta atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg
     7021 gcaggtgtcc gcggtatagt tgagatcgcc atacaggccg ccaccagagc caaccccgtt
     7081 atcgccaaag aaataattgc gatggatttt gatacgggag aagaatgtcc ctactgagcc
     7141 atcgttgaag ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gcgcacgatg
     7201 ggtcaccagc acccctttgg gtgtatttat caagcctact gtgccgctat caatatcttc
     7261 ctgtttgcaa cccaaaagtg acagtaacgc aggggcaatt gttaaaccag caaggccttt
     7321 tcctgatata cgcaagaatt ctaatcgcgt taagccaata tttaatagtg gacgatcaac
     7381 cgggttcatt tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagcctgat
     7441 attaggcact caactaatac gctgctgact ctgtcctcaa caccagatat tttccctaac
     7501 ccaatagtgt atgagaaaag atttaacgct gataaactaa tcctttatgg ttaggagaaa
     7561 aaataatgtg attattctgg gctcttaaca ttgatcgtta tcaattaaa
//
LOCUS       DAZGAP010000210         7557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGAP010000210 DAZGAP010000000
VERSION     DAZGAP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.211"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..428
                     /gene="chaB"
                     /locus_tag="ACHLAM_004484"
     CDS             198..428
                     /gene="chaB"
                     /locus_tag="ACHLAM_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO7855917.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            586..1281
                     /gene="chaC"
                     /locus_tag="ACHLAM_004485"
     CDS             586..1281
                     /gene="chaC"
                     /locus_tag="ACHLAM_004485"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO7855918.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(1325..1678)
                     /gene="ychN"
                     /locus_tag="ACHLAM_004486"
     CDS             complement(1325..1678)
                     /gene="ychN"
                     /locus_tag="ACHLAM_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO7855919.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1864..3258
                     /gene="ychO"
                     /locus_tag="ACHLAM_004487"
                     /gene_synonym="iatA"
     CDS             1864..3258
                     /gene="ychO"
                     /locus_tag="ACHLAM_004487"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO7855920.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3259..3909)
                     /gene="narL"
                     /locus_tag="ACHLAM_004488"
     CDS             complement(3259..3909)
                     /gene="narL"
                     /locus_tag="ACHLAM_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO7855921.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3902..5698)
                     /gene="narX"
                     /locus_tag="ACHLAM_004489"
     CDS             complement(3902..5698)
                     /gene="narX"
                     /locus_tag="ACHLAM_004489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO7855922.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSARFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            6037..7428
                     /gene="narK"
                     /locus_tag="ACHLAM_004490"
     CDS             6037..7428
                     /gene="narK"
                     /locus_tag="ACHLAM_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO7855923.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1764 a         1845 c         1990 g         1958 t
ORIGIN      
        1 ggatatcggt aacattttac gaatttttac cccgctgtcg attttttact catttgggca
       61 taaaaataag taatacgttt agacaatgtt tgttttagca cttcttaaga agagtctgac
      121 atgaaaattc ttatgttttg gcaagagtag atattgttga ccacacttaa tgttcaactt
      181 tgtaaaagga gtcaacgatg ccgtataaaa cgaaaagcga tctgccggaa agcgtaaagc
      241 acgtgcttcc gtctcatgcc caggatatct ataaagaagc gttcaacagc gcatgggatc
      301 aatataaaga taaagaagat cggcgtgatg acgccagtcg cgaagaaaca gcgcataaag
      361 tggcctgggc tgctgtgaag catgaatatg ccaaagggga tgatgataaa tggcataaaa
      421 aatcgtaaaa ccggtagctt agttaaagct attcgtgcgg tgttgccttg caagtggtcc
      481 gtggattgca tattgtcccg ttagtggttt caaaatgagc agtaaaaatg tccggaagac
      541 accaaaaagt tgtcgcaggg aagtatgcag tggcggaggt gtaaggtgat aacgcgtgat
      601 ttcttgatga atgccgattg taaaacggca tttggtgcca ttgaagaatc actcttatgg
      661 tcagcagaac aacgggcggc ttcgctggcg gcgacgctgg cttgtcgacc tgatgaggga
      721 ccggtgtgga tcttcggtta tggatcgttg atgtggaatc cggcactgga gtttaccgag
      781 tcgtgcaccg gtacactggt tggatggcat cgtgcgtttt gcctgcgcct gaccgccggg
      841 cgcgggactg cgcaccagcc gggacggatg cttgcactga aagagggcgg acgcaccaca
      901 ggcgtcgcct atcgactgcc agaagagacg ctggagcagg aactaaccct gttgtggaag
      961 cgcgagatga ttaccggctg ttatctgcca acctggtgtc agcttgatct tgatgatgga
     1021 cgcacagtaa acgccattgt gtttattatg gacccgcgac atccagaata tgaatctgac
     1081 actcgcgctc aggtcatcgc gccgttgatt gcggcggcga gcggtccact gggaaccaac
     1141 gcacaatacc tgttttcact ggaacaggag cttatcaaac tgggaatgca ggacgatggg
     1201 ctgaatgatt tgctggtatc ggtaaaaaaa ctgctggcgg agaattatcc ggatggtgtg
     1261 ttacggccgg gactcgcctg agtaaacttc cctcatagtg gggcgtcaga cgccccctca
     1321 aacattaaaa tgtgagcact ttatcggctg acagcgtcca ttgcgccagt tccacaagcg
     1381 taccgatttc caccccatca atcagaggaa gtgtactaat cccgcgcccg tcggtacagg
     1441 ttttgcacaa tttcaccggt acattctgag cggtaaggat ctccagcatt tgctgaatgt
     1501 tgtagccttc ccctggtttt tgcccgcgca acccggctgt gaccgcatca gacatcagga
     1561 acagacgcag atccagattg ctctcctgct ctcgtaacgc aatggccagc cgcaagctgt
     1621 taaataagga ttcgctcccg taaggtgcgc cattggcaac gatcacgatt ttttgcatta
     1681 tttactcctg tattcaggga attagacact catcttctat cttactgctt ctgcagcgtc
     1741 tgaccaatcg gtcacatttt taaggatttt cctgaaagcg cgagaaaata cgacaaaagt
     1801 tgccagtaat cgttattctt tagggctatg gtttttcatt ttttaccgga agttaccgac
     1861 gttttgagcc gtttcgttcc tcgcattatt ccgttttatt tactcttgct ggcggcaggc
     1921 ggtacagcta acgcacaatc taccttcgag caaaaagcgg caaatccctt tgataataac
     1981 aatgatggtc tgccggattt aggcatggca cctgaaaatc atgatgggga aaaacacttt
     2041 gctgaaattg tgaaagattt cggcgaaacc agtatgaatg ataacgggct ggatactggc
     2101 gagcaggcaa aagctttcgc attgggaaaa gtccgcgacg cgtttagtca acaggttaat
     2161 cagcacgtag agtcgtggct atcagcgtgg ggaaatgcca gtgttgacgt caaagtggat
     2221 aacgaaggac atttcaccgg cagtcgtgga agctggtttg tgccgttaca agataatgat
     2281 cgttatctca cctggagcca gcttggtctt actcagcagg atgatgggct ggtgagcaat
     2341 gtgggcgttg ggcaacgctg ggcgcgcggc agctggctgg tgggttataa cactttttat
     2401 gacaacttgc tggacgaaaa tcttcagcga gcgggcttcg gtgccgaagc gtggggcgaa
     2461 tatttgcgac tatcggcaaa cttttatcag ccgtttgctg catggcatga acagacagcc
     2521 acgcaggaac aacggatggc gcgcgggtac gacctgacag cccggatgcg catgccgttt
     2581 tatcaacacc tcaataccag tgtcagcgta gaacagtatt ttggtgatcg tgttgatttg
     2641 tttaactctg gtacgggtta tcacaatccc gtcgcgttga gtctgggatt aaattatacc
     2701 ccagtgccat tagtcactgt gacggcccag cataaacagg gtgaaagtgg cgaaaatcaa
     2761 aataacctcg ggctgaatct taattaccgc tttggtgtac cgctcaaaaa acaactttct
     2821 gcgggcgagg ttgccgaaag tcagtcgtta cgtggtagtc gctatgacaa tccgcagcga
     2881 aataatctac cgactcttga gtaccgacag cgaaaaacgt taacggtgtt tctggcgaca
     2941 ccgccgtggg atctaaaacc tggcgaaaca gtgccgctga aattacaaat ccgcagtcgt
     3001 tacggtattc ggcaactgat ttggcagggc gatacgcaga tattaagttt gacgccgggc
     3061 gcacaagcca acagtgagga gggctggacg ctgatcatgc ctgactggca aaacggggaa
     3121 ggcgcaagca atcactggcg attgtcagtg gtggtggaag ataaccaggg gcagcgtgtc
     3181 tcctccaatg agatcacgct aacgcttgtc gaaccgttcg acgcattgtc aaacgacgaa
     3241 ctgcgctggg aaccgtaatc agaaaatgcg ctcctgatgc acccataccg cagcttccac
     3301 gcgagacttt agcttcattt tcttcagcat gtgcttgacg tgaactttta ctgtgctttc
     3361 ggtgatgtcc aggcggcggg caatcatctt gttcggcaaa ccctgggcaa ttagcttgag
     3421 aatatcgcgc tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg cacggttagc
     3481 gcgcaagctg gcggccagaa caggcgttaa tgcttcgctt aataccattt cgccagcagc
     3541 tgcctgatgt aacgctttca gcagatcttc cggttccata tcttttaaca gatagccatc
     3601 cgcgccgcgt ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc tgaataccac
     3661 aatgcgcccg gagagggact tttcgcgcag tttatccagc gtttccagac cgttcatgcc
     3721 gggcatattg agatctaaca ggatcagatc gggatcaaga gactccgcca gttcaatacc
     3781 ctgttcgcca ttactcgctt cgccaaccac ggtgatatct ggtgccatac tgataagctg
     3841 ttttacgcca gttcgcagca tcgggtgatc gtcaatcagc aggatagtag ccggttcctg
     3901 attactcatg ggtatctcct tggacgtctg tgaaagtttt ttcgggaata aaggtgacca
     3961 ccacttcggt gccacctgat tcacgacggc ggacgcggca atcgccccgt aaactttgcg
     4021 cacggtcgcg cattattatc atgccgtagt gattgctgcg gatggcattt tcaggcacgc
     4081 cgcagccgtt atcctggacg gtcagtttga cctgattatc gttttgcgcc actgtcacca
     4141 cgacctcact cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg gcaatttgca
     4201 acagatggat tgcctgatgc gaaggcacca gacgaggcgg caattgataa tccagcttca
     4261 ccgggaagcc aaacctggcg ctgtactctt cgcaactcgc ctccagcgcc ggacgtaatc
     4321 caggctcggt gagctgcaag cggaatgtgg tgagcaattc acgcaactgc gcccaggatg
     4381 cattcagttc gttacggatc tgacttaaca gttcgcggct gctttctggc agcgcatcgc
     4441 cctgcatctg taaacaactc acctgcatct tcatgcaaga gagagattgg gcaatagaat
     4501 catgcagttc gcgcgcaatg gtggcacgct cttccatcac gatcaactgt tgctgacgtt
     4561 cctgatggcg atccagcgca agcgtggcgg tgagttgttc aaccagggta tccaccagtt
     4621 gttgttgatc atgactaaga tgacgcccct gcggcagggt tgccagcaaa ataccgtact
     4681 gcgtatgaga gtcagccagc cgccacttca gggttgtgcc gcgatcgcca acggggagta
     4741 caccgcgcgg gcagagctga cagcctttat catcgcaggt catatctggc tggcaggtaa
     4801 actcctgatg attctcttca tcatcagtgt catacacccg cagttcgata tcacgtagca
     4861 gggttaaatt ctgtaagccg ttgagtacag gtgacaggcg ttcacacagc ggggcgcggg
     4921 aatgcaaacg gcggttggcc tgccataaaa aagagaggat ctgattttta tgttccagcc
     4981 cggcggtttt ctcctgaacc cgctgctcaa gtacggcgta actttccgcc agttctgcag
     5041 acatattgtt caacgcagtt ccaagcatag ccatttcgtt gcgcccgctg atgttcgcgc
     5101 gttgggtaaa atcgcgatgg ctgacggcac tcgccattgc caacaactgc cgccacggtt
     5161 gtagcagtcg cgcccgcaac cagataatag tgaacaccag taaaagtgcc ataaataccg
     5221 ccattacccg atggaccagc accactgtct caatgcgcat ttccgtggtg cggtcaaaac
     5281 cagataccag ttgatcaagc ccggcaacaa actggctgac atccgctgac accgtttcgc
     5341 ggttttgtgc acgcattagc gcagggatca gttcattacg ccagtaatct tgtaaaccct
     5401 gtaattgcgc cagttgtccg tctcgttctg ctgctcgggt taactcggcg ctaaatgctg
     5461 tttgttccat ctctttaatt aagggcttgt ctttctcgct taatggcact gctgccaaca
     5521 gacggtaact ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg ctgccctgaa
     5581 cgccttgcac cagccagcca gaaactgcca tccctgccag tccaatagca gtagaaagca
     5641 acacaataag cgcaacctga ttaaccaggg tgagcggaga gagacaacgt ttaagcatgc
     5701 aaacctcttc cttcaggctt taaatgagca ataaccttaa tgaatgtgac gatacattct
     5761 ggaatggcag tattctcggc tattggctga agtataccca tacccggaaa gagttactcc
     5821 ttatttgccg cgtggttagt cgctttacat cggtaagggt agggatttta cagcaccgtg
     5881 aaaaatctca taatttttat gaaatcactg tactcactat gggtaatgat aaatatcaat
     5941 gatagataaa gttatcttat cgtttgattt acatcaaatt gcctttagct acagacacta
     6001 aggtggcaga catcgaaacg agtatcagag gtgtctatga gtcactcatc cgcccccgaa
     6061 agggctactg gagctgtcat tacagattgg cgaccggaag atcctgcgtt ctggcaacaa
     6121 cgcggtcaac gtattgccag ccgcaacctg tggatttccg ttccctgtct gctgctggcg
     6181 ttttgcgtat ggatgttgtt cagcgctgtt gcggtgaact taccgaaagt cggttttaat
     6241 tttacgaccg atcagctatt tatgttgacg gcgctgcctt cggtttctgg cgcgttatta
     6301 cgtgttccat actcctttat ggttcctatc ttcggtggtc gtcgctggac ggcgttcagc
     6361 actggtattc tgattattcc ttgcgtctgg ctgggttttg ccgtgcagga tacctccacg
     6421 ccttatagcg tcttcatcat catctctctg ctgtgcggct ttgctggcgc gaacttcgca
     6481 tccagtatgg caaacatcag cttcttcttt ccgaaacaga agcagggtgg cgcgctgggt
     6541 ctgaatggtg gtctgggcaa catgggcgtc agcgtcatgc agttggttgc tccgctggtg
     6601 gtatcactgt cgattttcgc agtatttggt agccagggtg tcaaacagcc ggatgggact
     6661 gagctgtatc tggcgaatgc gtcctgggta tgggtgccgt tccttgccat cttcaccatt
     6721 gcggcgtggt ttggcatgaa cgatcttgct acctcgaaag cctccatcaa ggagcagttg
     6781 ccggtactca aacggggtca tctgtggatt atgagcctgc tgtatctggc aaccttcggc
     6841 tccttcatcg gcttctccgc gggctttgcg atgctgtcaa aaacgcagtt cccggatgtt
     6901 cagattctgc aatacgcttt cttcgggccg tttattggtg cgctggcgcg ttctgcaggt
     6961 ggtgcattat ctgaccgtct gggcggaact cgtgtcacac tggtgaactt tatcctgatg
     7021 gcgattttca gcggcctgct gttcctgacc ttaccgactg acggacaggg cggaagcttc
     7081 atggcgttct tcgcagtctt cctggcgctg ttcctgacag ctgggctggg tagtggttcc
     7141 acttttcaga tgatttccgt gatcttccgt aaactgacaa tggatcgcgt gaaagcagaa
     7201 gggggctctg acgaacgtgc gatgcgtgaa gcggcaaccg acacggcggc ggcgctgggt
     7261 ttcatctctg cgattggcgc gattggtggc ttctttatcc cgaaagcgtt cggtagctcg
     7321 ctggcattaa cgggttcgcc agtcggcgca atgaaagtat ttttgatttt ctatatcgcc
     7381 tgcgtagtga ttacctgggc ggtatatggt cggcattcta aaaaataaat cttaatattt
     7441 gattatcctt tgcgcggcat gatgtcgcgc tttttttacc tgtcatttag ttacaacatc
     7501 cttatgaagt gtggcgtatt tcgccggttt tcattaagag ccattaatat gttaccc
//
LOCUS       DAZGAP010000211         7539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGAP010000211 DAZGAP010000000
VERSION     DAZGAP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.212"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..282)
                     /gene="pdeL"
                     /locus_tag="ACHLAM_004491"
     CDS             complement(<1..282)
                     /gene="pdeL"
                     /locus_tag="ACHLAM_004491"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO7855924.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNN"
     gene            complement(1159..3192)
                     /gene="betT"
                     /locus_tag="ACHLAM_004492"
     CDS             complement(1159..3192)
                     /gene="betT"
                     /locus_tag="ACHLAM_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO7855925.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            3321..3908
                     /gene="betI"
                     /locus_tag="ACHLAM_004493"
     CDS             3321..3908
                     /gene="betI"
                     /locus_tag="ACHLAM_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO7855926.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            3922..5394
                     /gene="betB"
                     /locus_tag="ACHLAM_004494"
     CDS             3922..5394
                     /gene="betB"
                     /locus_tag="ACHLAM_004494"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO7855927.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            5408..7096
                     /gene="betA"
                     /locus_tag="ACHLAM_004495"
     CDS             5408..7096
                     /gene="betA"
                     /locus_tag="ACHLAM_004495"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO7855928.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
BASE COUNT         1945 a         2010 c         1944 g         1640 t
ORIGIN      
        1 attattaccc gtggcggata tgatctccgg attgtactga aagaaaatat cccgccagaa
       61 agtaatatcg ctctgaatcc ccagtttctc aaagagctgt ttcttttgat gtgaaatggt
      121 ctttgcacta cgatttctgt actgtgatat ttctgttaca gacattccct gcatcagcag
      181 cttgagtagc agtcgttctt tctcggatac gctaccaggc aatgacaaat gaaccgttgt
      241 accgaactct tgcagaaaaa cacgaaaatc acatgaattc atgaacacac cttttatctt
      301 ttatcgctgt tcagtatgag tgattacctg tggattcctg agcacacata aggggtaacc
      361 ataaacatta gcgatgccat acgtataaca tcccacatca ctatcaagaa gattatcttt
      421 acctctgatg ctcgctcaga aacagttacg ctaaatacct taaaaaggta atcacaagtc
      481 aaaactcagg agataatcac gcaagaatgc aatattaaaa ttcagatact catttttggt
      541 aataaagaag tagttggact atttcgatat ttataatttc ccactacgta ttgacgccca
      601 ttcatgagta aaggcgcatt atatgaattt aacaatccag atcaactgaa aatacctcaa
      661 cctcataaac acatagcgtc aacattcaat gacataatga gtaaagatgt tggttatgca
      721 tacacgtcat tactctatgc ctatccctta acaacctgcc tcttaagact gaatccattc
      781 agcaatagat gaccaacatt ataacaatga caataaaaac aatatttaaa acaatcaata
      841 aaatatcaga cacagacaat taaaccttaa acaacaaatg gttacattga tttttgttga
      901 tttgcgctac gcgttcccca tgaataaagc gtcatttcaa attagcacca atatttacat
      961 taattaagac taagcttatt gaacattcac aacagtccaa acgtaggttt tgggcttcaa
     1021 tagttctttt cttttttatt atctgttgcc tggcaatcat caatctatac taagggctta
     1081 actgtacaga tataacgtat caagaggtaa gagaaaccgc gccatatgca tacatggcgc
     1141 ggttttatgc aataacaatc acgcgtccgg gaacatcacg ctatggcccg gcgcttcacg
     1201 atggagatga ataaagttaa ggtgccgctc gtactggtca agaatatcgg tgatcacctg
     1261 ctctttgctg tagtccatca ggtcgttgcc ctggctgcct tctaacagga aggtttccag
     1321 ccggtagtag gtcgatttac cgctacgtgc gcggtaggta aagcccggca ccgaatattg
     1381 ctgcggccaa atctgataga caaagttttg ttcttcgccc atatgcacca acaaatccag
     1441 atgccccaac tgttgtccct cttccggcgg caggcttttt agctccacgt atgcgccgcg
     1501 caaccgcagc tcctgcgcca cttcttccat tgccgggtaa cagaccgtct ccatcatctg
     1561 tttagtgtaa cgcgtgcccg gataattcat caggcgcgag agacgttttt tccagctcag
     1621 gcgatcctga agccccagcg gtcgcggcgc ggtatcgcgg ttcgcacttt cacggcggta
     1681 atcttctacc ttcagagatt tatacaaccc cgccatcacg aagaaaatca caaagctgaa
     1741 cggcagcccc atgattaccg tggtgttttg cagcgcggat atcccgttgg tcatcagcat
     1801 gccgagcgtc agcaggccaa tcgccaccga ccagaagacg cgcagccagc cgggggcgtc
     1861 gctgttgata tctttaagct gcgaggtgaa attccccagc accagcgcac cggagtccgc
     1921 cgaggtcaca taaaacagca ggccagtaat ggtggcgacg gaggcgctaa aggtaaacgc
     1981 cggatattgc gccagcaggc tgtagaagcc gcgctccgga tggaccatcg cttcctcggc
     2041 aaatgccgcg ccgccgtgga tgatttcata cagcgcgcta ttgccgaata ccgagagcca
     2101 taacagcgtg aaggtaaacg gaataatcaa cgtgcccagc acgaactggc gaatggtacg
     2161 cccacgcgag atacgcgcca ggaacaagcc gacaaacggc gaccatgcca cccaccatgc
     2221 ccagaagaag agcgtccagt tattcatcca ctcaaccgga cggtcgaagg caaaactgtt
     2281 gagcgtcatg cccataaagc gattcacata gtcgccaaca ttcagcacca gtgcattaag
     2341 caggaacgag gtgtcgccca taaacaacac gaacaggatc aatcccagcg ccagcgcaac
     2401 gttgagttcc gataacacgc gaatgccctt atcaacaccg gatgtcaccg agatagtggc
     2461 gatgatcacc gacaaggcga tcagtgccgc tttcgccgcc atcgaatcgg gaatatcaaa
     2521 cagtacgctc aagccatagt taagctgcac cacaccgata ccgagcgtag tggcaatacc
     2581 gaagatggtg ccgatcaccg ctgcaatatc cactgaatga ccaatcggcc cgttaatccg
     2641 tttaccgaag atcgggtaca gcgccgagcg gatggtgagc ggcaaattat aacgataact
     2701 aaagtatccg agcgccatgc ccatcagcgc atacatcgac cagccggtta agccgtagtg
     2761 aaacagcgtc cagaccatcg cctgacgcgc ggcctcaatc gtctgtcccg cgccttccgg
     2821 cggctgcata tactgcgtta ccggttcggc tacggagaag aacatcaggt cgataccgat
     2881 cccggcagca aacagcatcg ccgcccaact cagcagactg aattccggtt tggattgttc
     2941 tggcccgagc ttcaccgaac caaaacgcga acaagcgata cagaccacaa agacaatata
     3001 gagcgttgcc gccagcagat agtaccaacc gaaggtttta gaaacccagt ccagcgtgcg
     3061 gccaatccac agggccgaga agtcgcgaaa caggatcgtt gtcagggaaa acaacaaaat
     3121 cagtccggcg gaggtgtaaa acaccaccgg attgattttg tccttttccc tgctgtgtga
     3181 aaggtctgtc atccagtatc cccactgtta ttgttaccat ttaaaatcaa attcgtaaca
     3241 attaagacac attttatatt gaacgtccaa tcaataaccg ctttaataga taaacaacgc
     3301 tgatgaatgg agtggcgaaa atgcccaaat tggggatgca gtcgatccgg cgcagacaac
     3361 tgatcgacgc cacgctggaa gcaataaatg aagtgggcat gcacgatgca acgatcgcgc
     3421 agatcgcccg ccgtgcaggc gtttctacgg ggatcatcag ccactatttc agggacaaaa
     3481 atggtctgct ggaagcaacc atgcgcgata tcaccagtca gctgcgtgac gcggttttga
     3541 atcgattaca tgcacttccg cagggcagtg cagagctgcg attacaggcg attgttggtg
     3601 gaaacttcga tgaaacccag gtgagcagtg cggcgatgaa agcctggctg gccttctggg
     3661 ccagcagtat gcatcagccg atgctctatc gcttgcagca ggtcagtagc cgccgcttat
     3721 tgtcgaatct ggtgagcgaa tttcgccgcg agttaccgcg ccaacaggca caggaagcgg
     3781 gctacggcct tgccgcgctg attgacggat tatggctgcg cgcggcactg agcggcaaag
     3841 cgctggataa acctctcgca cactcgctga cccgtcactt tattactcag catttaccca
     3901 ccgattaacc gaggaaacgt gatgtcccga atggcagaac agcagcttta tatacatggt
     3961 ggttatacct ccgccaccag cggtcgcacc ttcgagacca ttaacccggc caacggtaac
     4021 gtgctggcga ccgtgcaggc cgccgggcgt gaggatgtcg atcgcgccgt gaagagtgcc
     4081 caacaagggc aaaaaatctg ggcggcgatg accgctatgg agcgctcgcg tattctgcgt
     4141 cgggccgttg atattctgcg tgaacgcaat gacgaactcg caaaactgga aaccctcgac
     4201 accggaaaag cgtattcgga aacctcaacc gtcgatatcg ttaccggtgc ggacgtgctg
     4261 gagtactacg ccgggctgat cccgtcgctg gaaggcagcc agatcccatt gcgtgagacg
     4321 tcatttgttt atacccgccg cgaaccgctg ggcgtagtgg cagggattgg cgcatggaac
     4381 tacccgatcc agattgcctt gtggaaatcc gccccggcgc tggcggcagg caacgcgatg
     4441 attttcaaac cgagcgaagt caccccgctt accgcgttaa agctggccga aatttacagc
     4501 gaagcgggcc tgccggacgg cgtatttaac gtgctgccgg gcgtaggtgc ggaaaccggg
     4561 cagtatctga ccgagcatcc gggcattgcc aaagtgtcat ttaccggcgg tgtcgccagc
     4621 ggcaaaaaag tgatggctaa ctcggcggct tcctccctga aagaagtgac catggagctg
     4681 ggcggtaaat caccgctgat cgttttcgac gatgcggatc tcgatctcgc cgctgatatc
     4741 gccatgatgg cgaacttctt cagctccggt caggtgtgta ccaacggcac ccgcgtgttt
     4801 attccggcga aatgcaaagc cgcatttgaa caaaagattc tggcgcgcgt tgagcgcatt
     4861 cgcgcgggcg acgttttcga tccgcaaact aactttggcc cgctggtcag cttcccgcat
     4921 cgcgataacg tgctgcgtta catcgccaaa ggcaaagagg aaggcgcgcg cgtactgtgc
     4981 ggcggcgatg tactgaaagg cgatggcttc gataacggcg catgggttgc accgaccgtg
     5041 ttcaccgatt gccgcgacga aatgaccatc gtgcgcgaag agatcttcgg tccggtgatg
     5101 tcgcttctca cttatgaatc tgaggacgaa gtgattcgcc gcgccaatga taccgactac
     5161 ggcctggcgg cgggcatcgt gacggcggac ctgaaccgcg cgcatcgcgt cattcatcag
     5221 ctggaagcgg gtatttgctg gatcaacacc tggggcgaat cccctgcaga gatgcccgtt
     5281 ggcggctaca aacactccgg cattggtcgc gagaacggcg tgatgacgct ccagagttac
     5341 acccaggtga agtccatcca ggttgagatg gctaaattcc agtccatatt ctaaccagga
     5401 ggtttatttg caatttgact acatcattat tggtgccggc tcagctggca acgttctcgc
     5461 tacccgtctg actgaagatc cgaacacaac cgtgctgctg cttgaagcag gcggcccgga
     5521 ctatcgcttt gacttccgca cccagatgcc cgccgcgctg gcgttcccgt tacagggtaa
     5581 acgctataac tgggcctatg agacggaacc ggaaccgttt atgaacaacc gtcgcatgga
     5641 gtgcggacgc ggtaaggggc ttggcgggtc atcactgatc aacggcatgt gttacatccg
     5701 tggtaacgcg ctggatctcg ataactgggc gaaagaaccc ggtctggaga actggagcta
     5761 cctcgactgt ctgccctact atcgcaaggc cgagactcgc gatgtgggcg agaacgacta
     5821 ccacggcggt gatggcccgg tgagcgtcac cacctccaaa cccggcgtca atccgctgtt
     5881 tgaagcgatg attgaagcgg gcgtgcaggc gggctacccg cgcacggacg atctcaacgg
     5941 ctatcagcag gaaggttttg gcccgatgga tcgcaccgtc acgccgcagg gccgtcgcgc
     6001 cagcaccgcg cgtggctatc tcgatcaggc caaatcgcgc cctaacctaa ccattcgtac
     6061 tcacgccatg accgatcaca tcatttttga cggcaaacgc gcggtgggcg tcgagtggct
     6121 ggaaggcgac agcaccattc cgacccgcgc ggcggcgaat aaagaagtgc tgttatgtgc
     6181 aggcgcgatt gcttcaccgc agatcctgca acgctccggc gtcggcaacg ctgaactgct
     6241 ggcggagttt gatattccgc tggtgcatga tttacccggc gtcggcgaaa atcttcagga
     6301 tcacctggag atgtatctgc aatatgagtg caaagaaccg gtttccctct accctgccct
     6361 gcagtggtgg aatcagccga aaatcggtgc ggagtggctg tttggcggca ccggcgttgg
     6421 tgccagcaac cactttgaag caggcggatt tattcgcagc cgtgaggaat ttgcgtggcc
     6481 gaatattcag taccatttcc tgccagtggc gattaactat aacggctcga acgcagtgaa
     6541 agagcacggc ttccagtgcc acgtcggatc gatgcgctcg ccaagccgtg ggcatgtgcg
     6601 gattaaatcc cgcgacccgc accagcatcc agcgattctg tttaactaca tgtcgcacga
     6661 gcaggactgg caggagttcc gcgacgcaat tcgcatcacc cgggagatca tgcatcaacc
     6721 ggcgctggat cagtatcgtg gccgcgaaat cagccccggc gcggaatgtc agacggatga
     6781 gcagctcgat gagttcgtgc gtaaccacgc cgaaaccgcg tttcatccgt gcggtacctg
     6841 caaaatgggt tatgacgaga tggcagtggt tgacgccgaa ggccgcgtac acgggctgga
     6901 aggcctgcgc gtggtggatg cgtcaattat gccgcaaatt atcaccggca atttgaacgc
     6961 tacgacgatt atgattggcg agaaaatggc ggatatgatt cgcgggcaag aagcgttgcc
     7021 gaggagcacg gcaagatatt ttgtggcaaa tgggatgccg gtgagagcga aaaaaatgag
     7081 tcgtgatgtg aactaactca ggaacctatc agattgagag ttaccgttct actatgagga
     7141 ccgaatcctt atataaaaca ctgaggtaac tctcatgctt catatccgca gtcccgccat
     7201 caaactttaa gccgattcgc tcaatctggc ggatgatggt gctatggata cgctgataaa
     7261 ccacaagtgt aatgccactc agcttaagaa tcacgcaacc agtgctcctg gctagggtta
     7321 cttgttcttt cccactcccg gttagcagtg gtcagcgatc aactgcgtaa gccgggcgta
     7381 tttatcccaa agcgtggagt aatatcaggt ctacgctaaa aaagtcaatt tattcggtta
     7441 tcatgcacga aaaggggact tgtggcgagt ataaaaataa gtcagcccca aaaactacgt
     7501 ggagctttaa acacggttcg tataatttat tagcagcga
//
LOCUS       DAZGAP010000212         7498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGAP010000212 DAZGAP010000000
VERSION     DAZGAP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.213"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            166..4416
                     /gene="fdeC"
                     /locus_tag="ACHLAM_004496"
     CDS             166..4416
                     /gene="fdeC"
                     /locus_tag="ACHLAM_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO7855929.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGITVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEALVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPAKLVLDPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4541..5398)
                     /locus_tag="ACHLAM_004497"
     CDS             complement(4541..5398)
                     /locus_tag="ACHLAM_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7855930.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARMNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5647..6516
                     /locus_tag="ACHLAM_004498"
     CDS             5647..6516
                     /locus_tag="ACHLAM_004498"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO7855931.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6676..7269)
                     /gene="rclC"
                     /locus_tag="ACHLAM_004499"
     CDS             complement(6676..7269)
                     /gene="rclC"
                     /locus_tag="ACHLAM_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO7855932.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIIFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7281..>7498)
                     /gene="rclB"
                     /locus_tag="ACHLAM_004500"
     CDS             complement(7281..>7498)
                     /gene="rclB"
                     /locus_tag="ACHLAM_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO7855933.1"
                     /translation="FATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLENAIADMA
                     RKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         2046 a         1660 c         1933 g         1859 t
ORIGIN      
        1 ggctacagac actggatata ttaggtggcg gtgtactttc ttacataaag gtatttcctt
       61 ttctgtggaa aaggaaaccg ggaaatcccc ggtttttctg acaagcagac gccattattt
      121 gtgtctgcct atgttcgtta attcgttcat caggaaatta tctcaatgtc acgttataaa
      181 acagacaata aacagccacg atttcgttat tcagttctgg cccgctgcgt ggcgtgggca
      241 aatatctctg ttcaggttct ttttccactc gcagtcacct ttacgccagt aatggcagca
      301 cgtgcgcagc atgcggttca gccacggttg agcatggaaa atactacggt aactgctgat
      361 aataacgtgg agaaaaatgt cgcgtcgctt gccgctaatg ccgggacatt tttaagcagt
      421 cagccagata gcgatgcgac acgtaacttt attaccggaa tggccaccgc taaagctaac
      481 caggaaattc aggagtggct cgggaaatac ggtactgcgc gcgtcaaact gaatgtcgat
      541 aaaaatttct cgctgaagga ctcttcgctg gaaatgcttt atccgattta tgatacaccg
      601 acaaatatgt tgttcactca gggggcaata catcgtaccg acgatcgtac tcagtcaaat
      661 attggttttg gctggcgtca tttttcagaa aatgactgga tggcgggggt gaatactttt
      721 atcgatcatg atttatcccg tagtcatacc cgcattggtg ttggtgcgga atactggcgt
      781 gattatttga aactgagcgc caatggttat atccgggctt ctggctggaa aaaatcgccg
      841 gatgttgagg attatcagga acgcccggcg aatggctggg atattcgtgc tgagggctat
      901 ttacctgcct ggccgcagct tggcgcaagc ctgatgtatg aacagtatta tggcgatgaa
      961 gtcgggctgt ttggtaaaga taaacgccag aaagatccac atgcgattac cgctgaagtg
     1021 aattacacgc cagtgcctct tctgaccctg agtgccgggc ataagcaggg caagagtggt
     1081 gagaatgaca ctcgctttgg cctggaagtt aattatcgga ttggcgaacc tctggaaaaa
     1141 caactcgata cagacagcat tcgcgagcgt cgaatgctgg caggcagccg ctatgacctg
     1201 gttgagcgta ataacaatat cgttcttgag tatcgcaaat ctgaagtgat ccgtattgct
     1261 ctgcctgagc gtattgaagg caagggcggc cagacggttt ccctggggct tgtggtcagc
     1321 aaagcaactc acggtctgaa aaatgtgcaa tgggaagcgc cgtctttgct ggccgcaggc
     1381 ggaaaaatta cggggcaggg caatcagtgg caagtgacgc tcccggctta tcaggcaggc
     1441 aaagacaatt attatgcgat ttcagcgatt gcctacgata acaaaggcaa tgcctcgaaa
     1501 cgtgtgcaga cagaagtggt tattagcgga gctggtatga gcgccgatcg tacggcgtta
     1561 acgcttgacg gtcagagccg tattcaaatg cttgctaacg gtaatgagca aaagccgttg
     1621 gtgctgtctc tgcgcgacgc cgagggtcag ccagtcacgg gcatgaaaga tcagatcaag
     1681 actgaactaa ccttcaaacc ggctggaaat attgtgactc gtaccctgaa ggccactaaa
     1741 tcacaggcac agccaacact gggtgagttc accgaaactg aagcaggggt gtatcagtct
     1801 gtctttacta ccggaacgca gtcaggtgag gcaacgatta ctgttagcgt tgatgacatg
     1861 agcaaaactg tcactgcaga actgcgggcc acgatgatgg atgtggcaaa ctccaccctg
     1921 agcgctaacg agccgtcagg tgatgtggtt gctgatggtc agcaagccta cacgctgaca
     1981 ctgacagcgg tggactccga gggtaatccg gtgacgggag aagccagccg cctgcgactt
     2041 gttccgcaag acactaatgg tataaccgtt ggtgccattt cggaaataaa accaggggtt
     2101 tacagcgcca cggtttcttc gacccgtgcc ggaaacgttg ttgtgcgtgc cttcagcgag
     2161 cagtatcagc tgggcacatt acaacaaacg ctgaagtttg ttgccgggcc gcttgatgca
     2221 gcacattcgt ccatcaccct gaatcctgat aaaccggtgg ttggcggtac agttacggca
     2281 atctggacgg caaaagatgc taatgacaac cctgtaactg gcctcaatcc ggatgcaccg
     2341 tcattatcgg gcgcagctgc tgctggttct acggcatcag gctggacgga taatggcgac
     2401 gggacctgga ctgcgcagat ttctctcggc actacggcgg gtgaattaga cgttatgccg
     2461 aagctcaatg ggcaggacgc ggcagcaaat gcggcaaaag taaccgtggt ggctgatgca
     2521 ttatcttcaa accagtcgaa agtctctgtc gcagaagatc acgtaaaagc cggtgaaagc
     2581 acaaccgtaa cgctggtggc gaaagatgcg catggcaacg ctatcagtgg tctttcgttg
     2641 tcggcaagtt tgacggggac cgcctctgaa ggggcgaccg tttccagttg gaccgaaaaa
     2701 ggtgacggtt cctatgttgc tacgttaact acaggcggaa agacgggcga gcttcgtgtc
     2761 atgccgctct tcaacggcca gcctgcagcc accgaagccg cgcagctgac tgttattgcc
     2821 ggagagatgt catcagcgaa ctctacgctt gttgcggaca ataaaactcc aacggttaaa
     2881 acgacgacgg aactcacctt caccgtgaag gatgcgtacg ggaatccggt caccgggctg
     2941 aagccagatg caccagtgtt tagtggtgcc gccagcacgg ggagtgagcg tccttcagca
     3001 ggaaactgga cagagaaagg taatggggtc tacgtgtcga ccttaacgct gggatctgcc
     3061 gcgggtcagt tgtctgtgat gccgcgagtg aacggccaaa atgccgttgc tcagccactg
     3121 gtgctgaatg ttgcaggtga cgcatctaag gctgagattc gtgatatgac agtgaaggtt
     3181 aataaccaac tggctaatgg acagtctgct aaccagataa ccctgaccgt cgtggacagc
     3241 tatggtaacc cgttgcaggg gcaagaagtt acgctgactt taccgcaggg tgtgactagc
     3301 aagacgggga atacagtaac aaccaatgcg gcaggtaaag tggacattga gcttatgtca
     3361 acggttgcag gggaacttga gatcgaggcc ttggtgaaaa actctcagaa gacggtcaag
     3421 gtgaaattca aggcggattt cagtaccggt caggcgagcc tggaggtaga cgccgctgct
     3481 caaaaagtgg caaacggcaa agatgccttt acgctgacgg caacggttaa ggatcaatac
     3541 ggcaaccttc ttcctggcgc tgtggtcgtc tttaatctgc ctcggggcgt caaaccgctt
     3601 gcagacggta atatcatggt gaacgccgac aaggagggta aagcggaact gaaagtggtt
     3661 tccgtgactg ccggaaccta tgagatcacg gcgtcagcag gaaatgacca gccttcgaat
     3721 gcgcagtctg taacgtttgt ggctgataag actacggcga ccatctccag tattgaggtg
     3781 attggcaacc gtgcagtggc ggacggcaaa accaaacaga cgtataaagt tacggtgact
     3841 gatgccaata acaacctgct gaaagatagc gaagtgacgc tgactgccag cccggcgaag
     3901 ttagttctgg atcccaatgg gacggcgaca acgaatgagc aagggcaggc tattttcacc
     3961 gccaccacaa ctgttgcggc gacatataca ctcacggcgc aagtgagtca gaacaacggt
     4021 caggtatcaa cgaaaaccgc cgaatctaaa ttcgtcgcgg atgataaaaa cgcggtgctc
     4081 gctgcatctc cagagcgtgt agattctctg gtggcggacg ggaagactac tgcaacactg
     4141 acggttactc tgatgtcggg tgtcaacccc gtaggaggaa ccatgtgggt cgacattgag
     4201 gctccggaag gggtgacaga ggcggattat cagttcctgc cgtcgaaaaa tgaccatttc
     4261 gcgagcggga aaatcacgcg tacatttagt accaacaagc caggtacata cacattcaca
     4321 ttcaactctt tgacatatgg agggtatgaa atgaaaccag tgactgtgac aattaacgcc
     4381 gttcctgcag atactgaagg cgctgaggag aaataaaaac gacgatgtaa taaataataa
     4441 tggcgttgac atgcgcagcg ccattattta ctgaatacct accctggcac cgattagacg
     4501 gtggcagggt agggtaaatc ttatgtgatg gagttacagg ctaaaccggg cgaacgggga
     4561 taaaaatttc gcaaacaatg ttattgctat caatttcgac gaagttttca ttgtaataaa
     4621 atcgctcaat atcactgccg ttcattctgg ctaaccccct ggcaggtaat tccatcagat
     4681 agaggtttcg cgcccattca gggtagtgct cccactcacc ttcatagcgg aaagaggcat
     4741 acagtcctcg gttaattgtc cactctttcg acatctctga atcggtttgt ttgcataccg
     4801 taccggcaaa agtttcaact gtgagcaaat cgaccgattt tgttgatggt tcgaacgtgg
     4861 agacgaaata gatatccgtc ttaaaactct tcatacatct ggctaaatga tgacgtcgca
     4921 gtttgacttc ttcatccggt gatttattcg acacttcaat atcatattta aatgaattac
     4981 caaaaatagg tgtctcagga aagttgacta atctacactc cgtcttttgc tgttgacgta
     5041 ttaaaaaaga agggaagata tttgcgagat cccaataatc acggtggcgg tattcacggg
     5101 gagagcagcc gaataacttt ttaaattcgc ggctgaatga ctgctgtgag tcaaaatgca
     5161 aagagagtgc aatatcaagc atagatttcg cggagagccg gacaagaatg gcggcacgac
     5221 aaagccttcg tttgcgaatg tattctccca aaggcacatg catgaaattt cggaacagaa
     5281 gctggatgtt gcgtctgctg tagccagatt tctgtgcgat atcttcgatt gaaatagggt
     5341 gttcgagatt gcactcaatc cactccagga gctgctgtag aatcttctgc ctgatcatca
     5401 cattctccgt taattctttg tcatatcgca cattttcgtt atgccctggg ccctaagtgt
     5461 atcaaaaaca gcaagggcga aggcaaaagc actggtagcg tctgcttcgt tgtccccgaa
     5521 aatagagagg tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc
     5581 cacatactaa tttgaggtta cgttttaacg tagactcatt gttcatgcct aatggaggga
     5641 ctgacagtgg aattttccgt attaagtaac aatctgaaaa tgccgatgat gggatttggt
     5701 gtttttcagg ttaccgataa aaacgtgtgc aaacagtcag tgttgaacgc tatccgcacg
     5761 ggttatcgac tcatcgacac tgcagcggta tacggtaatg aagatgccgt cggtgaagcc
     5821 gttcgtgaag ctatatctga aggtttatgt acccgagaag agttatttat aacgtctaaa
     5881 ttgtgggtgc aggatatgtt gaatcaggat attgcagcag caggtattga agcatcatta
     5941 aaaaaatcgg gactagaata cttcgatctc tatttattgc accaggccat gcgcgattat
     6001 tttagcgcgt ggcgtgcact cgaagatgcc tatgaagagg gcaaattaaa agcgattggg
     6061 gtttccaatt tctaccctca tgttctggcg aacttttgtg aaacggtaag agttaaaccg
     6121 atggtcaacc aggtcgagtt gcatccctat tttgcccaac cagaggcgct ggcaaccatg
     6181 aagtattata acgtgcagcc tgaagcatgg gctccgttag gtggtggacg acataaaccc
     6241 tttgaaaata atctgcttca gagtattgca gatgcccatc aaaaatcgat ttctcaggtc
     6301 attctgcgtt ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag
     6361 cgtatcgaag aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcgcaa
     6421 attagttcgc ttgatttggg ttatgttggg gaatcggtaa aacattttaa tcctgagttt
     6481 gttcgtggtt gtcttgctgt aaaaatacat gattgatatt aatcacttat tttacctgag
     6541 acgacaagaa tcttttaaca ggggagtgat attgatcttc actctgtcat atctccggta
     6601 atatggcgtc aggcttcatc atcagaaagg gggcctgacg cctgaaaaag tgaacaacag
     6661 acagtgttcg gattatcaat attcagtttt taacgttgaa ctggattcat tactgcgttg
     6721 tttaagaata tcccgcgccg aatctgccat tatcatgaca gcacctgcca gcatcagagt
     6781 atctttcaat accagacgac cagcaccgga taaataaggg aaaccatgat gagcgtcacc
     6841 caatgcgggt acccatgcct ccggggtggt gattaaaaat gagagtgtta ccagcggcgt
     6901 ggtaaatgcc atcagcccac ccaataaacc taaccagcga ttgacaggat tagccaaaac
     6961 cagcagagca ataatcacct ccacgacgcc aagaccgttg gaaaaaccat aggtattatt
     7021 agccgtttgc catgctcttg cttctggttt gtattcgcct tcgtgagtca gatgctgttt
     7081 atagtcttcc gggtgttcat aaaagaacga cattagtgga ctgtttgcga cgaatggtgt
     7141 aatgctgtct gcctcgtaag ggacaaactt taataaccca atccacataa aaataatcgc
     7201 aatactcaga cgaatcaatg tcaggccaat tttatctccc cgacttaaca ggtgcaggta
     7261 tctttccatt gtaaacatcc ttatttatac aatactgctg ttgcggttga attattatca
     7321 attcgcatcg atgtaacttt ccaggatgaa gcattatatt ttctggccat atcagcaatg
     7381 gcattctcaa gaactgtcgg tgatgctgat actgatgaga cgctgatatg tttcagaatt
     7441 attttatcat ctgcattggt ggaaaatgcc ataacgccag ataatagtgt tgcaaata
//
LOCUS       DAZGAP010000213         7425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGAP010000213 DAZGAP010000000
VERSION     DAZGAP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.214"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..690)
                     /locus_tag="ACHLAM_004501"
     CDS             complement(<1..690)
                     /locus_tag="ACHLAM_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO7855934.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRR"
     gene            complement(701..1702)
                     /gene="ltaE"
                     /locus_tag="ACHLAM_004502"
     CDS             complement(701..1702)
                     /gene="ltaE"
                     /locus_tag="ACHLAM_004502"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO7855935.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1739..3457)
                     /gene="poxB"
                     /locus_tag="ACHLAM_004503"
     CDS             complement(1739..3457)
                     /gene="poxB"
                     /locus_tag="ACHLAM_004503"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO7855936.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3590..4558)
                     /gene="hcr"
                     /locus_tag="ACHLAM_004504"
     CDS             complement(3590..4558)
                     /gene="hcr"
                     /locus_tag="ACHLAM_004504"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO7855937.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4570..6222)
                     /gene="hcp"
                     /locus_tag="ACHLAM_004505"
                     /gene_synonym="priS"
     CDS             complement(4570..6222)
                     /gene="hcp"
                     /locus_tag="ACHLAM_004505"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO7855938.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6366..7265)
                     /gene="lysO"
                     /locus_tag="ACHLAM_004506"
     CDS             complement(6366..7265)
                     /gene="lysO"
                     /locus_tag="ACHLAM_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO7855939.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1798 a         2026 c         1936 g         1665 t
ORIGIN      
        1 gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac tgagcacttc
       61 tggtccggcg gcttcgaaga tgcgatgttc gctggccgga tgatctaaca gcgccaccag
      121 atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgaaccc agcgtggcgg
      181 cgttaacacc ggcaggttgt agaccatatc gcgcatgact tcgaacgccg ctgaacctgc
      241 accaacgatt attccggcac gtagttcggt caccgggaca cccgcttcac gaagaatatc
      301 cgccgtagcc tgacgggcgc gcagatggtc agactgctca tgtgccgggg cctgcaacga
      361 actgaggaag attaattgct taacagggac ttcgcgtagc gcatcgcgga cgttgagagc
      421 cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca ccagaaaata
      481 gaccgtatcg atatcctgca acagggccgg aaggttatcc ggccagctga gatcgacttt
      541 atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac gtgccgccgc
      601 caggatctga tgcccttgct ggctgagagt tcgcaccaga tgctgaccaa tgtagccact
      661 ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc aggaacgcac
      721 gccagtgggc ggcaacttcc gccagttgtt cacgcgagac gtcaagatgg gtcaccaggc
      781 ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg cctaacgcgg
      841 cagcattttc ttccccgacg cgaacaaaca gcatattggt gtcctgacgc atcacatccg
      901 cgcctgcttc acgcaactgc tccgccatcc aggcggcgtt atcgtggtct tcctgcaaac
      961 gtgcgacgtt atttttcagg gcatacatcc cggcggcagc cagaatgccg gactgacgca
     1021 tcccgccacc tgtcattttc cgccagcgaa tggcacgttt aatgtaatca cgattaccga
     1081 cgagtaatga accgaccggc gtcccaagac ctttcgacag gcaaatggtg aacgaatcac
     1141 aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta aagatgcgcg
     1201 caccgtcaac atgcagcgcc agattgcgct cgcgagtaaa ttcccatgct tctttcaggt
     1261 attcccgcgg caacactttg ccgttgtggg tgttttccag actgagtaat ttggtgcggg
     1321 cgaaatggat atcgtcgggt ttgatcttca tcgccacttt atccagaggt agcgtgccgt
     1381 cggcagccgc gtcgatgggt tgcggctgaa tactgcccag cactgctgcg ccaccggctt
     1441 caaacagata gttatgcgcg gcctgaccga cgatatactc ttcgccgcgt tcgcagtgac
     1501 tgagcagagc gaccaggttg gcctgagtgc cggtaggcag aaaaatggcg gcttctttac
     1561 cggaaagctc tgctgcatag tcctgcagag cattaacggt agggtcgtct ccgtaaacgt
     1621 cgtccccaac cggggcggcc atcatcgctt cgagcatggc gcggctcggt cgggtaacgg
     1681 tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc cacccttttt
     1741 accttagcca gttcgttttc gccagctcga tcacttcatc gccgcgtccg ctgataattg
     1801 cgcgcagcat atacaggctg aaacctttgg cctgttcgag tttgatctgc ggtggaatgg
     1861 ctaactcttc tttggcgacc accacatcca ccaacaccgg accgtcgatg gagaaggcgc
     1921 gttgcagggc ttcatcgact tcagaggctt tttctacacg aatacccgta atgccgcacg
     1981 cttcggcaat gcgggcaaag ttggtgtcgt gcagctcagt accgtctgtc aggtagcctc
     2041 cggctttcat ctccatcgcc acaaagccca gcacgctgtt attaaagacg ataattttca
     2101 ccggcagttt catctgcact actgagagga aatcgcccat caacatgcta aaaccgccat
     2161 cgccgcacat ggcgaccacc tgacgttccg gctcggttgc ctgcgcaccc agcgcctgcg
     2221 gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc ttgccgttca
     2281 tttttagata acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg aaaatagcgt
     2341 catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg gctttctcgc
     2401 tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc agcgctttat
     2461 ccagaaactt gcgatcggct ttttcttcca ccaatggcag caatgcacgc agagtcgact
     2521 tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg gctgggttga
     2581 tatcaatctg aataattttg gcatcggtcg ggtagaacgc acggtaggga aactgagtac
     2641 cgagcagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgaa aagccgatta
     2701 atcccgtcat tccaacatca tatggattat cgtattcgac atgttcttta ccgcgcaggg
     2761 catggactat aggcgcttta attttcccgg caaactcaac taactcttta tgcgcccccg
     2821 cacagccgct gccacacatc agggcgatat tgctggaata acgcagcagc tgcgccagtt
     2881 tgcgtaactc ttcttcttcc ggtgttacta ccggctgtgg cgcatgatac cagtgggtag
     2941 ttgccccttc tggcgcaggt tttaacgcca cgtcgcccgg taacacaaca acggaaacgc
     3001 cacggttaag caccgctttg cgcatagcaa ttgccagcac ttgtgggatc tgctctgggc
     3061 tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg tgggtttcct
     3121 ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc agtaccggaa
     3181 cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg ggaccgcacg
     3241 atccggcaca gaccgccagt tctccgctaa gttgtgcttc agcgccagcg gcaaatgccg
     3301 ccacttcttc atgccgggtg gacatccact cgatggtgcc catgcgatta agactgtcac
     3361 taagaccgtt cagagagtcg cccgtgactc cccagatgcg tttcacgcct gccgattcga
     3421 gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc ctgaatgtga
     3481 taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagaa aaaggaggca
     3541 ctaacggtta aatagcccga tgaaaggaat atcatcgggc atcaggcgat tatgcgagaa
     3601 ccaaatcccc ctgcggatgg caggagcagg ccagtacata accttcagcg atttcggcgt
     3661 cggtcagcgt cattgtgctg ctcaccgtat attcaccgga aaccactttc gtcttacagc
     3721 agccgcaaac acctgcgcgg caggcggcga caaccggaac gttattgctt tccagcgcct
     3781 ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt ttggtgaatt
     3841 tcagaccgct ggtcgccgct tccgctactg gggtgaagaa tttctcttta aagaaacgcg
     3901 tcacgccgag cgctttcact tcctgctcta cccaatccat atatggagca gggccacagg
     3961 tcatcacggt acgtgaggct aaatcaggga cacctgccag cagttcgcga gtgagacgac
     4021 cagcgataaa gccttcggta acgttatttt ccgccaccag cgttaccgga tagttacgcc
     4081 actcatcggc gaaaatcaca tcctgcggcg tacgcacgtt gtagatcacc tgcacatcgg
     4141 cctgtggacg gttcttcgcc agccagcgac gcatcgacat aatcggcgta acgccgcagc
     4201 ctgccgccag caacaggaat ttatcttctg ctttatcatc gcaggtaaat tcccccatcg
     4261 cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac tgggagccga
     4321 caccgtcatc aatccgccgc acagtcaggg tgatgtattc actcacgcct ggcgtggagg
     4381 aaatggtgta agcacgcagc gtttccgctg agttacgcac gctgaccagt gcatattgcc
     4441 cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca tccggcgttt
     4501 cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc atcgtcatcg
     4561 acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg tgatagaacg
     4621 caggccgaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac caggtgcagt
     4681 cgggccggtg acgatatttt tcacacccag agaaagcagc gtcagcagaa tgacaatcgc
     4741 tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttgactc cgcagcccag
     4801 tttctctgcc agcgttaccg ccagaataat tgctgagtaa gcatcgttac attgaccagc
     4861 atctaccaga cgcggcagac cttcgatatc gccaaactca agtttattaa agcgatattt
     4921 accgcaagcg agggtcagga tcaggcagtc atccggcacg ctggtggcga aatcggtgaa
     4981 atagtgacgc tcgccgcgtg cgccgtcaca gccgccaagc aggaagatat gacgcagttt
     5041 ttcacggctc accagatcaa tcagcgtatc ggctgcgcca agcagtgtct ggcgaccaaa
     5101 gcccacggtg atcaggtgcg gaatttcgct gtacgggaag cccgccatct gttgcgcctg
     5161 ggcaatgact gcagagaaat cttcgccatc cagatgacgc acacccggcc agccaacgat
     5221 gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga tgcagttcga
     5281 ggtcatcacg atggggcctg ggaaacgagc gaactccact tgctgattct gccagccgct
     5341 gccgtagtta ccgaccagat gcttgaattt acgcaactcc gggtagccat gcgcaggcag
     5401 catttcgccg tgggtgtaaa cattaacgcc cgtgccttcg gtctgttcca gcaggttgta
     5461 gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg ctttgacgtt
     5521 gacctgagtt ggcgtcgggt gaccgtattt accggtttcg cctgcgtcca gaatgctcat
     5581 cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt tcatatcggc
     5641 aggccaggtc cccagccacg ccatgatttt atggtactgg gcataaatat cgttgtcgta
     5701 ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc catacaggca
     5761 cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt taggggtaaa
     5821 ttctgctgcc tgacgttgca gctcgccgag atcgtcgctc accagttgca ggtcagccat
     5881 cgggttatcg acgcgggcgt tggcatctac agccaggcat tgtgctttca gcgcctcgcg
     5941 cagggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt taacgttggt
     6001 cagggttgag aaaaaggcac gtggcgcaaa gctgtctaca tcgtggttga taatgccgta
     6061 ttcacgtgct ttcactgccc aggccgaaag cccttgcagc gccgcgatga gtaaatcctg
     6121 aaggtcagag gtttctgccg ttttaccaca catcccctgc gcgtatgagc agccgtttcc
     6181 tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac cttttaaagt
     6241 tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa gagattttta
     6301 atgcaacttt aagggagatt gatttagcgc aattttggcg gcagagatct accgccagag
     6361 aggtattatg cagagaaaaa ggcgatgaga atcggcacta acagactaag aataaaaccg
     6421 tgaacaatcg ccgccgggac catatccagc ccgcctgtac gttgaagaac gggcagggtg
     6481 aaatccatcg acgtcgcacc gcataagccc agtgcagtag agcggctacg gcgaatcagc
     6541 ccagggatta acataatagc aatcagctca cgggccagat cattaaaaaa cgccgcgctc
     6601 ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata ccagccgaaa
     6661 ccggaggcca ttgccagcgc ggtattgatg ggaagatcaa gaataaaggc gttaattaaa
     6721 ccaccaatta atgaactggc aaccaccacc acggcgacaa tcattccccg gcgattaagg
     6781 acaatctgct ttaaggtcat gccattattg cgcaactgaa taccgacgag gaaaagtagc
     6841 agaattaagg tgtattcact ggcttcggtc gcgtgttgta agaaagccag tccactcaga
     6901 ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag cgccatcgca
     6961 atacgcgacg ggagtttttc ttgatgatgg tggttgcgcc atggcaggcc gcgctccagc
     7021 cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc agaataatgc
     7081 agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat acccataaaa
     7141 aagagaataa ggtaaaccat ccagcttaat agctgattaa taactcttaa tgcagctttt
     7201 tggcgaagcg gaatgaggta acccacaatc aggggaacca gaatgattaa cagcccagaa
     7261 aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca cactactgaa
     7321 agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcatcaga gggagatttt
     7381 taattctata tgagtgttta gcattgttgc gctcctttca tgtgc
//
LOCUS       DAZGAP010000214         7393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGAP010000214 DAZGAP010000000
VERSION     DAZGAP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.215"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(525..1415)
                     /locus_tag="ACHLAM_004507"
     CDS             complement(525..1415)
                     /locus_tag="ACHLAM_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO7855940.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            complement(1416..4388)
                     /gene="nfrA"
                     /locus_tag="ACHLAM_004508"
     CDS             complement(1416..4388)
                     /gene="nfrA"
                     /locus_tag="ACHLAM_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO7855941.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRH
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPRKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGIEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4375..6612)
                     /gene="nrfB"
                     /locus_tag="ACHLAM_004509"
     CDS             complement(4375..6612)
                     /gene="nrfB"
                     /locus_tag="ACHLAM_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO7855942.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            complement(6810..7393)
                     /locus_tag="ACHLAM_004510"
                     /pseudo
     CDS             complement(6810..7393)
                     /locus_tag="ACHLAM_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1738 a         1900 c         1882 g         1873 t
ORIGIN      
        1 ttttagttta attgtctgtt tgtgttttgc attgtggttt tatgggctaa ttattttttc
       61 tttgtggagt ttttatctat tcaagtacca tgcctttaga tgcatattag cgataatagc
      121 atgaggttta tcctcaattg ctgtgttttt tagtataaaa aagaggaaca aaactgagac
      181 acataaggcc tcgcaatggc ttgcaaggtt ttacatattt tgaggtggtg gaacgtgtga
      241 acgcagagat ggcgcggtaa tttgttgatt taaaatgtcg ttcctggagt ttctaagtcg
      301 tgggctacag gttcgaatcc tgcagggcga gccatttcct cgccatttat ttgttccctt
      361 ttacaaatta caccatcact ttgttgtcgc ggttttgcta aataattgga acacgtcaga
      421 cgaataaaat gaaaacagaa attctgctaa acaattcatc ttgccaggat tttgattagg
      481 aaagtgaaaa ggaaaggagc aagctaagtt gctcccttta atcattaata ctcaaggata
      541 ctctgcgcga cgggcaaata gcgcagagag aaatagagcg tgtctttacc gcaagaggaa
      601 cgtttcgcta acagcgaggc gatctctgca tccggtttcg gtttcgctgt aaacgtttta
      661 cctttaccag cgacaaacag ttcataaaca atgccgcttg ctggggcgga actttggcaa
      721 tcggcgctgg cctgtagata acgttcacgc tgttcagcgg tcagtttatc cacgccgctg
      781 ccatcctgta cccagacatt aacaccggtt gctttaactt gttccagcag ttggcgatag
      841 ccatctggcg acatgtttcc ggcgaaaaaa ctactgatat aaaccggttt tgctgaaaca
      901 tcgctaatca accgctgcgc gttgtttaac catgttagca aaggttgacg ggcggcttcg
      961 ctgcgccagt tcaggtcgtc aatttccgcg ctgatgtacc agccatccgg cgttacgcca
     1021 ggcacggcgc tccataatct ggcttgctgg agatcggcag ccagcaggcg attaagatag
     1081 ctttccagcg ctgcggagga ctgtttctgg tgtataaaaa attccggatc ggcgttcagc
     1141 ccgacaatca gcttcaggcc agcctgttgc gcagctgcgg cacgcttaaa caataacgcg
     1201 cgctgttctg gctgggtgaa tgcatcgccg taacgggtcc attgcaaaac cagggtatcg
     1261 aagccttgca aacgtaactg actcatcagc ccctgccact gggtatcggt aacctgacta
     1321 tcacggtttt gtggttgcca gataatacct ttcatcgcaa aggaaaaagg gctgaccaaa
     1381 agcagtgtca acaatacgaa aatgaactta cgcatttacc agtgcactcc aatggtgaga
     1441 aacgcgttgt tgcgctctcc gttacgttga ttaatcgcct taaaggtatg ttgatattcg
     1501 atgccgagac tgactttgtg cggccaggcg tcgtagtgcg tctcgccggt ccagatattc
     1561 cagcggaccc cgacaccgcc aagctgcgcg ccctgagtgc ctttatcacg ataaccgttg
     1621 tcctgaacgt gagcataagg ctcaatagtc tgcccgtttg ccaccttctg atgccagctg
     1681 actcgataat ctgccgtcca cgcctgaata tcctggcgga tatattgcgc cgcatcgagg
     1741 tacaggtttt gggcaaacca gcctgaaccg ttcgggtgcc attcgtcgct gtatttgccg
     1801 ccattaaaga atgaggcgct ggcgcgcagc atggtatcgg atgcgccatt ttgtccgttc
     1861 agcggcaact gctgttcgac ggcgaggaaa aagatctgat cgcgcagcgg cttccagcgc
     1921 agaccggtgc cggacatcgg atttttcacc ggcatcatca ccccgttttc accggtatcg
     1981 gcaaagacgc ggctataaac tgagagcagg tcgccttcca gcagcatatt gcgtccgatg
     2041 cggtactcgg cttccagttg cccgtagcta cgatagcttt tccttggcgc tgcgccgccg
     2101 acattattgt tagcggtact cattgcgccg gaacgcaagc caatagaaga atcgaaactg
     2161 aacgtccagt ggcgaccgac ctcctcatgc agacggcgga aattgaagcg ttgctgattt
     2221 tgctctgggg tcagtggggt tatcagcgcc tgattatcaa tgtcatcaat caccagtcgg
     2281 gcgtagtgct gcgtcgcagg catgtcatcc agacgctggt tcacgtaggc cagttgtcgg
     2341 ataagtgccg gatcgtccgg tagcccttta tgcgcctgtt cgagcatttc ccgcgactgt
     2401 gcgatatcac cgctatccca caaggcgtaa ccgagcgctg cctgggtgtt gctattattc
     2461 ggttccagtt ccagagcggc gcgcaaatca ctcaccgccg ccgggacatt atgacgttgg
     2521 cgataaattg tcgcccgcgc aacgtaagcg ttggcagaag gcgcaatatt gattgagcgc
     2581 gtgagatcgc tcagtgcgag ttccggctga ccaggaatgt aacgttgcgc atgcagccac
     2641 cagtagaggg cattgtttcc cagcccacgt tgttctgcct gctgaagcca gcgatcgcga
     2701 gccgcaccat ttcctgccgc ctgggcggta ttggcagcag caagcagatc ctcattgctc
     2761 atgccgtgaa gactgatttt ctgccaggcc gccagtgcgg tggcgtagtc ctcaacctga
     2821 tacgcctgat aggctaccgc acgatgttgc caggcgttcg gttgtcgttg ttcggcctga
     2881 agccatgcat acaacgccac accgggtagc gtgtcccgat aacactttgc cagacggttc
     2941 caggcggcag catcgtagga aggcgacata tcgcccagca agcgaactat tgccgggcaa
     3001 ttatctgcaa tacccggcaa ctgactttgc cactgacgtt gctccgccag cggtaagggt
     3061 ttcgataaaa tcgccacctt cgccggagtt gccaggtaag gatgactttc cagcagagac
     3121 gccagtcgcg ccattaaagt ctggctgaca cgcgcatcgc cctggaaagg atagcgttgc
     3181 agcaataaat cggcagcttc acgtgactgc tcgttctgca tcagttgcca ggtcagttga
     3241 tccaggcggg taagatttgc cggttcttgc tgatacagca atcgtgccag acgcagagct
     3301 tcagccttgt tacgggtcgc cacgctaacg gtataacgct cctcaagcat ttcattggcg
     3361 gggagggtgg cgagcagttt ttgcgctgcg tcgtactgac cttcttttaa cagcaccggt
     3421 agcgtcgcgc caacaacata ctggcggttg tcggcaaact gtaccgtata attcgccaac
     3481 gcctgaacgg ggttgccgct gtatttagat aacagataga gccaactttt ctcttgtgcg
     3541 tccgtggtaa atagcggcgt attttcaatg agataatgct ggaggcgtgc tttttcgcca
     3601 cgataagcca gcgcggtcgc gtaagtaata tatgactgag gatcggtgaa gatcccctgt
     3661 gattgcagcg ccaggatccg atcgtccagc tgcccggcaa gaagcacatc aaaccactga
     3721 cggcgttctg ccgcgcttaa tgtgttctgc tggcgtgctt cattgtatag cgtatctgcc
     3781 tgggaccatt gtttcaggta gattgcccgt tgcagcagat cggttcgcag cgtttttcct
     3841 tccggcgatg cagcaaacgt cgcatcgttc agttgcgctc tggcgacagg taattgtgcc
     3901 agccgcaggg cattctgccc gacttcactg cgacaacgca gggtcggcgc agcatcgcac
     3961 gctttttgct gggcaagcag ttcttcaaca gtcgtaacgc ttttcacttc aactggaata
     4021 gccgccagac tgcgctcaag tcgggcatct cctgggtgac gtttcagttg atcctcaagc
     4081 aacagccgcg cccggtcatc atgaccaaaa tggcgatagg cttccgcaag gtataaagtc
     4141 agcggaatat tatccggcac ctgttggtgt atatattcaa attcgcggat ggcggttgct
     4201 tcgtcgttat ttttctgtgc cttcagcgcc ttatcgagac ggggataaat aacaaaatgg
     4261 cgataatcgc tcagccccag ctcttctgcg ctggtgccga tattgtctgc gagtgcgctg
     4321 gtgctcaata aagacgtcag cagtaaacca gaccatccga tgacgcgatt aaggttattc
     4381 tccttcattt tcggactcca gttgcgcaac ctgttctggg tttaaacctg ctttgagtaa
     4441 tagtgattgc atcgaaactt gtaattcgcg ttgaattgtc aggacgcgat ccaacgtttc
     4501 ctggctgata acgccttcgg tgaccaaaaa cttgccgagc ggcagagaac tgcgttcatg
     4561 gcgcaataac aacacgttaa ttgctgaacg attaatatga ccgagcgtgg tcagtatttc
     4621 ggcgaacagg aactgatgcg gcacatattg ccgccagatt tcaccggcct gctgttccgt
     4681 gagccactga tgctgaaccg cattgtacaa cattgcccgc gggtcgtgac cgcgtcggcg
     4741 tgcataccag tgacgtaacc ccgtgacaat ttgtccgcgc agaacaatga cgtaacgcac
     4801 tttgcgtccg actttacgcg tcagggctgc cagcgaaacc gggtcaatac catcttcact
     4861 gccgacaatt aactcatcat tttccagacg cagcggcagc actgcataat gcagcgccac
     4921 ggaagccggc atttcggcaa tcagtgatga agggatctgc caggcgtcga tcgactccca
     4981 cgccacgccg ttttgctctg ccagcgcctg tgccagctgt tcggcgctaa tcagcccctg
     5041 catcagcatt gaaccgccca ggcgtagacc ttcgacgcga ttacgcagtg ctgtatcgag
     5101 ttgttcttca gtgatgacct gattttccag caggatttgg cctaacgggc gcaacgagcg
     5161 ggtatcgcca gtcacgctgg ggaagtcatg cgttgtttta tcccacgcga cgcgacgtgg
     5221 atcgccgtgt tgaagtacct gttttaacgc gcgccagttg gccatgaagt taatcaggtt
     5281 gccccagaaa agacgcagga cagatagcag cccctgcgtc agaccgtagt agccagtgac
     5341 aaaaatcacc cgctgcacga tgcggttaac catcaaacca aagtttagcc acagcagggt
     5401 cattaaccat gcgctgccgc tgaaaataga aaggaaatgc caggcatcgg gccacaaact
     5461 ttcatacgcc agtaacagca aaagctggat catcaccagc atcgcgagga agctgacaaa
     5521 gttactgatt gcccctttgc ggtcgcgcca gagaaagtag ttcagcgtca ggctggaggt
     5581 ccatttgtgg gttttaaagc cctggaaaac aatgccgatg atccagcgag atttttgtcg
     5641 aaccgcagtc gaaaaggtat cggggaaata ttcgcgcacg cagatcatgt ttgacgtccg
     5701 cgcgtgctgt aaaaatttac gctgctcgcg ttctttggct tcgtccacca ccggaaaacg
     5761 gacaaaaatt tccgtcatac ctttttcttt caggcggaaa ccaatgtcgt aatcttcagt
     5821 cagactctgc acgtcgaaag caataccgtc accgtcagct aacagtgcgg tcacggcgcg
     5881 gcggctgaaa caggtgccga cgcctgcgct gggcacctgt ccggcgaggg cttcacgcac
     5941 cggaacatct ttgccatgca gttctgaaaa ctcatcaatg taggtcatgc tggtgaagtg
     6001 cgtccattcg cgttcgaacg gatacactgg gatctgaatc agatctttac gctcgaccag
     6061 atagttgaac agacgcaatt ccatcggtga aatcacatct tcggcgtcat gcagaataaa
     6121 accagcaaaa gcgaaattgg cgctacgttc aaattgggtg atggcgtcca gcacgttgtt
     6181 cagacagtcg gctttgctgg tggggccagg acgcgcgcag actaccttat gcacgttcgg
     6241 gaagcgagcg cacacttcgt caacatcacg ctgagtatcg gggtcgttgg ggtaggtgcc
     6301 aacaaagata tgatagtttt cgtagtcgag cgtggtcgcc gccagctcgg ccatattgcc
     6361 gatgacgccc gtttcattcc acgccggaac cataatcgct aacggttttt catctggttt
     6421 atacagttcg cggtaactca ttcgcgggta gcggcgataa acactcaact tgcgtttaat
     6481 gcggcgtacc cagtagacga catcaataaa aaaatcgtcc agcccgctga tgaacatgat
     6541 gaccgctaac gttatcgcga ttacttttaa gccgtagagc caggtagcaa aaacatcaag
     6601 aagccagtcc acacaaaaac cttacattaa cgctggttat gtttagggtg gcgtatatta
     6661 aggtttttta tgaattgtga cagcttttta ccattaatag gtatgactat tgcggcacgt
     6721 tatttttaca ctggttataa aagttgccgt ttgcgtagaa tgtagacaaa agcgtggtgt
     6781 caaggagggg aggttaacgc agggaaagat tacgaaagcc cgctcccagc aaggactgac
     6841 gcgagatagt ttctgtccat ggctgctttt cgcaacttac gtcttaaccc tgccttgaat
     6901 accttatcat tcgtcaaaat attaatagcg atgtgccgta gccctgaaaa taattctgct
     6961 gcatttcctc ttcttatttt gcagtcgtct tcattcatta ccacgtccag acgccagtgc
     7021 agcttattct ccacgtgcca gtggtttcgg attgctgtgg cgaacttctc tgcggttaaa
     7081 tcagcagaac tgatataata tctgaccgtc atttctggct ctttcttttg ttctgctatt
     7141 attgaccgaa aggagactgc cacgcataat ttcttcagac ctttccatta aaacgtgaaa
     7201 tcaataagtt catcagggac atcgcaaaca atatgaagtc ggatttcttc tctgccgtga
     7261 ctcttttcac taattgcgta actgtcatgc tctggattat ttaattcttt cagtgggaat
     7321 ttttcctcaa aggctttatt aagccgcccc tgatttcctt ttacagcgaa taaataatca
     7381 ccgccctgtt ttt
//
LOCUS       DAZGAP010000215         7352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGAP010000215 DAZGAP010000000
VERSION     DAZGAP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.216"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..1231
                     /gene="anmK"
                     /locus_tag="ACHLAM_004511"
     CDS             122..1231
                     /gene="anmK"
                     /locus_tag="ACHLAM_004511"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO7855943.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLILSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1329..1658
                     /gene="mliC"
                     /locus_tag="ACHLAM_004512"
     CDS             1329..1658
                     /gene="mliC"
                     /locus_tag="ACHLAM_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO7855944.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1717..2373
                     /gene="pdxH"
                     /locus_tag="ACHLAM_004513"
     CDS             1717..2373
                     /gene="pdxH"
                     /locus_tag="ACHLAM_004513"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO7855945.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2502..3776
                     /gene="tyrS"
                     /locus_tag="ACHLAM_004514"
     CDS             2502..3776
                     /gene="tyrS"
                     /locus_tag="ACHLAM_004514"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO7855946.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3835..4698
                     /gene="pdxY"
                     /locus_tag="ACHLAM_004515"
     CDS             3835..4698
                     /gene="pdxY"
                     /locus_tag="ACHLAM_004515"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO7855947.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(4742..5347)
                     /gene="gstA"
                     /locus_tag="ACHLAM_004516"
     CDS             complement(4742..5347)
                     /gene="gstA"
                     /locus_tag="ACHLAM_004516"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO7855948.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5453..6955)
                     /gene="dtpA"
                     /locus_tag="ACHLAM_004517"
     CDS             complement(5453..6955)
                     /gene="dtpA"
                     /locus_tag="ACHLAM_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO7855949.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         1853 a         1793 c         1914 g         1792 t
ORIGIN      
        1 tgattattga tttgaagata atgttttact accaacggta aaaatcggtc attccgcatg
       61 gtattcacga aaagtttatg tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac
      121 tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc agccttgatg gtgttgatgt
      181 tgtgttggcg acaattgatg aacaccgggt cgcacagctg gcaagtttga gctggccgat
      241 cccggtgtct ctgaaacagg ctgtactgga tatttgccag ggccagcagc ttatactttc
      301 tcagtttgga cagcttgata ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt
      361 gcttaaggaa caaaacctgc aggcgagaga tatcgttgcg atcggctgtc acggtcaaac
      421 cgtctggcat gaaccgacgg gcgtggcacc acacacttta cagattggcg ataacaatca
      481 aattgtggca cgcaccggaa ttacggttgt cggtgatttt cgccgtcgcg atattgcctt
      541 gggaggacaa ggcgcaccgc tggtacctgc gttccatcat gcgctgctgg ctcacccaac
      601 cgagcgacga atggtgctca atattggcgg catcgccaat ctgtcactgc tcattcctgg
      661 gcagccggtt gggggctacg ataccggtcc tggtaacatg ctgatggatg cctggatctg
      721 gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg gcacgggcgg gtaaagttat
      781 tctcccactg ctgcaaaata tgctcagcga cccgtatttc tcgcaacccg caccgaaaag
      841 caccggacgc gaatacttta actacggctg gctggagcgc catttgcgcc attttccggg
      901 tgttgatccc cgtgatgtgc aggccacact ggcagaactc accgccgtga ccatttctga
      961 acaagttttg ttgagcggtg gctgtgaacg attgatggta tgtggtggag gaagtcgtaa
     1021 tccgctactc atggcgcgtc tggcggcatt actgccaggc acagaagtca ccaccactga
     1081 tgccgttggc attagtggcg atgacatgga agcattggct ttcgcctggc ttgcctggcg
     1141 gacgcttgcg ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt
     1201 actcggggct attttccccg ctaacccgtg atatcagagt taactgaatt tttcttaccg
     1261 cgtctgccgt tacacttgga acgttaggga gggcgtattg ccctccagac caggaaagtc
     1321 ttcgggatat gtctatgaaa aaactgttac tgatctgttt gccggtcctt ctcaccggct
     1381 gtagcgcgtt caatcaactg tttgagcgca tgcaaaccga tacgctggaa taccagtgtg
     1441 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
     1501 ataatcaact actgcatctc aaacagggca tttctgcgtc tggtgcacgt tacaccgacg
     1561 ggatctatgt tttctggtcg aaaggcgagg aagcgactgt ctataaacgc gaccgcatcg
     1621 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
     1681 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
     1741 caaatcgcgc atctgcgccg tgaatacacc aaaggcggat tacgccgccg cgatcttccc
     1801 gccgatccat taaccctttt tgaacgctgg ctctctcagg cgtgtgaagc caaactggcg
     1861 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
     1921 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggcagccgt
     1981 aaagcacatc aaatcgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
     2041 gagcgccagg tgatggtgat cggtaaagcg gaacgacttt cgactctcga agtgatgaaa
     2101 tattttcata gtcgcccacg tgatagccag attggtgcat gggtttcgaa gcagtccagt
     2161 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
     2221 cagggcgaag tgccattgcc gagtttttgg ggcggttttc gcgtcagcct tgaacagatt
     2281 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
     2341 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     2401 ctggtactcc tgattctggc actttattct atgtctattt cgcatctggc gaaaagtcgt
     2461 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     2521 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     2581 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     2641 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     2701 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     2761 ctgagcgtaa gctgaacacc gaagaaactg ttcaggaatg ggtggacaaa atccgcaagc
     2821 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     2881 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     2941 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     3001 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     3061 acaaacagta cggtgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     3121 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     3181 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     3241 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacaca gcggatgccg
     3301 acgtttaccg cttcctgaag ttcttcacct tcatgagcat tgaagagatc aacgccctgg
     3361 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtgctt gcagaacagg
     3421 tgacacgtct ggtgcatggt gaagaaggtt tgcaggcggc aaaacgtatt accgaatgcc
     3481 tgttcagcgg ttccttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     3541 gcgtaccgat ggttgagatg gagaagggcg cagacctgat gcaggcgctg gtcgattctg
     3601 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     3661 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     3721 gttttacctt attgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     3781 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     3841 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     3901 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     3961 aatcacactc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     4021 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     4081 tatctgggtt cggcggagca gggtgaacat attctcggta tcgttcgtca ggtgaaagca
     4141 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     4201 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     4261 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     4321 gaagcggttc tggcagcgcg tgaactcatt gcgcaagggc cacaaattgt gttggttaaa
     4381 cacctggcgc gggctggcta cagccgtgac cgttttgaaa tgctgctggt taccgccgat
     4441 gaagcctggc atatcagccg tccgctggtg gattttggta tgcgccagcc ggtaggtgtt
     4501 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     4561 gctctggaac atgtgaccgc tgcagtatac gaaatcatgg tgaccaccaa agcaatgcag
     4621 gaatatgagc tgcaagtggt tgctgctcag gatcgtattg ccaaaccaga acattatttc
     4681 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     4741 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     4801 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     4861 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatcg ttgcccgcag atccagtgct
     4921 catccttcag tgcctcgttc acatattgca gcttcttctc cagctgcgcg cgaactgtcg
     4981 atttgtactc ttccggtgta tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg
     5041 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     5101 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgctacg ccttccgtca
     5161 gcaaagtacc atcgtccagc agcaatgcag gtacctgtcc cttagggtta acggcaaagt
     5221 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     5281 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt ttgtagaaca
     5341 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     5401 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     5461 cggctgcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttcggcg
     5521 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatacgc
     5581 gaccatagac ttccagtgac atcagtggat cggtaacgtt atccggcaca gccatcatac
     5641 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     5701 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     5761 tcatcagttc tccgatactc tgcaggccat agcttgcgac cagccagctt acagacacga
     5821 tacctgcgtc agatgcgaat tttgcaccca gcggcagaat caggaacgca ccagaacaca
     5881 tcaccatacc gattgcaaac ttagttggca tcggcagggt atcgcccatc ttgttataga
     5941 tagcggccag aatcgggcta ccaatgatga tccagaacgg gttcagtgcc tgatactgct
     6001 caggttctac ggccaggccc agaatggtgt gttcaacgtt acgaatcgca aagaagttca
     6061 gtgacgttgg catctggctg tacagcacga agaagataat ggcttcgagc atcaggatga
     6121 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga
     6181 taaccacgat accaaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     6241 gcagccaggt ggcgatagcg atcagtgcca caacaccaat aatggtcagc agcaggttac
     6301 ggtagttgat aggctcgaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     6361 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     6421 agccgtattt cgcggccagc cacggggtgg cgatcataga gaagaaagag ccgatgttga
     6481 cggacatgta gtacatggtg aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg
     6541 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     6601 tacccatata aacgataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     6661 ccagcacaat agcgccaagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     6721 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     6781 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     6841 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     6901 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     6961 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgcc taaaaaacat
     7021 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     7081 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     7141 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactaccc
     7201 tgagaggggg atgatggctt aacggcgata tgttctgcta tttagcgttt ttcctagtga
     7261 gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata tttgttgcgt
     7321 gtgtaaattt aaatgatttt aacctagttc tt
//
LOCUS       DAZGAP010000216         7300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGAP010000216 DAZGAP010000000
VERSION     DAZGAP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.217"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(501..2105)
                     /locus_tag="ACHLAM_004518"
     CDS             complement(501..2105)
                     /locus_tag="ACHLAM_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO7855950.1"
                     /translation="MNPDYVQALQTSPHFPTDVTYRLFPSDLAYLIDDLYSSTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMTPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGDPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALAEGLNEHPEAGIISDEAVTFFRSYLKNNPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPNVFTDYINKNDVLAWGSGFLSRFLFSQVGGSSR
                     VRDYRKGEFVTIPTLVEFHKKINGFLSSHSINSPGMRSERKTLKLSKKALGEWQENQI
                     KIESKALAGGEWEHIRDIVLKAGSNILRIAGLFTCYCYKDDEEITSIALWKAMHLMEW
                     YLEEASTIFYPMSERCQFEQDARELYTWIMTRIKQNNLYAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLIAQNYLCIIKMRSHQALYVSVHCNNRYLLPCGAMSYEQFDIVPPLNSHN
                     AKTYHVNIPLALIPSFALPFSAYTLF"
     gene            complement(2535..3440)
                     /locus_tag="ACHLAM_004519"
     CDS             complement(2535..3440)
                     /locus_tag="ACHLAM_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7855951.1"
                     /translation="MALEQEIANLVNDFVAGGRPSCRDQTFDERRAGYIASTALAGDT
                     ETRVNISDVIIDDILLRIFSPLNDDKLSSAIVYYHGGCFVSGGFVTHDNQFRQLAFLS
                     GCRVIAVQYRLAPEHIYPAAHDDAEHAANIVWQHAEMLKVDRNKITLMGDSAGGHLAL
                     ITALRVKNIAQWLPRKLILIYPMLDATASCDSYKLNGSDYVLTEDTFLSGYEAYFSGL
                     DLEHPEASPLFRKDFNGLPPVHIITAEYDPLRDEGEELFSRMIQQGVKCTAQRYLGVI
                     HGFFQLGGISQTARNLMQDICGIVE"
     gene            complement(3894..4346)
                     /locus_tag="ACHLAM_004520"
     CDS             complement(3894..4346)
                     /locus_tag="ACHLAM_004520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO7855952.1"
                     /translation="MNVIDSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTKICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            complement(4860..5591)
                     /locus_tag="ACHLAM_004521"
     CDS             complement(4860..5591)
                     /locus_tag="ACHLAM_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7855953.1"
                     /translation="MNQSIHNAYWLSRFDSLLDSAPAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDIWCSLGDFSEPSSLATMIQQAWCSALHPEPWQGDGLVHFSRWTPSRKPA
                     AADASPSSDDTPLHSGCSDTRTDSDKRPGGSAVLWAKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGVRCLLDGR"
     gene            6262..6792
                     /locus_tag="ACHLAM_004522"
     CDS             6262..6792
                     /locus_tag="ACHLAM_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7855954.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAAMTITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
BASE COUNT         2189 a         1674 c         1453 g         1984 t
ORIGIN      
        1 ttacagaaaa atatcaaatc gtttttcttc aatcccctat tgaaagctaa acaacatctc
       61 ttatcagtaa cagactgata acctttcgtt attcagaaaa agatcgacat cgtgatatac
      121 gccagcactc atcacgacaa caatcaaaca aagttatata taaaaactca cataatatgg
      181 ttaataatta gcttcaatcc atgtgaaaaa taacatttcg attcactttc gagatatcaa
      241 aaacgaaaac aattcttcca ccagtgcaca cattcctgca ttcaaccagc tctgacaaca
      301 ggtcattatg aaaacaaaat caaatcacat acatggtaca tgtactgcta ccgttgctac
      361 cgtagcgatt ttttcagaga aattttcgtt cacaccccat aattggtgaa tatttgcaca
      421 aatgcaggat atgaaaaacg ccttaccatc aaccttcgaa tgaaggctta tgtgttttgt
      481 ttgcttattg taaagctgtt tcaaaataag gtataggctg aaaaaggtaa cgcaaatgac
      541 ggaattaatg ccagagggat attaacgtga tatgttttcg cattatgact attcaagggt
      601 ggaacaatat caaactgttc gtaagacatt gcaccgcaag gaaggaggta acgattgtta
      661 caatgaacag aaacataaag tgcctgatgc gatcgcattt tgatgataca taaataattc
      721 tgtgcgatta actgattgag tacaggtgta agtttttctg ctcttctcag acgattgggg
      781 ccatatctct caatgtctgt tttcctgata gcatacaaat tattctgctt gattcgggtc
      841 ataatccatg tatacagttc acgggcatcc tgttcaaact ggcatcgttc agacatagga
      901 taaaatattg tgcttgcctc ctccagatac cactccatga gatgcatcgc tttccaaagc
      961 gctattgatg taatttcctc atcgtcttta tagcaatagc aggtgaataa acccgctatc
     1021 ctcagtatat tagaacctgc tttcagaaca atatctctga tgtgttccca ttccccacct
     1081 gcaagcgctt tgctttcaat tttaatctgg ttttcctgcc actctcccaa cgctttcttc
     1141 gaaagtttta atgtcttcct ttcgcttctc attccggggg aattaatgct atggcttgac
     1201 agaaacccgt taatcttttt atgaaactcc accagggttg gtattgttac gaactcgcct
     1261 ttcctataat cacgtacccg ggaagagcct ccaacctgac tgaaaagaaa acgggaaaga
     1321 aatccgcttc cccatgccag tacatcattt ttatttatat aatccgtaaa aacattcggc
     1381 tgagacatta atgaaaatgt caggcgtgga gtaatatgat atttttcgtc tgctctccca
     1441 aaatcaaacg gttgtccact ccatgcttta ttcaacagac ccggattatt tttcagatag
     1501 cttctgaaaa aagttaccgc ttcatcagaa ataatccctg cctccggatg ttcgttgagt
     1561 ccttccgcaa gcgctttaag cgaaacatct tcatagataa aattcggtcg aaccggacgt
     1621 gtcggtttat ttctttcgtg attacgcagt gcctcttctt cctgttctcc cggatccccc
     1681 cggttaacag cctttcttaa attggcagca agagctttct gccgggtatt ccagatctgt
     1741 agttcagtct tataatcttt gtttctctct tcgtattgtt gaatgagtcg atcggcaaat
     1801 tcgtaacagg gggtcatcac cagtctgttt atcgttgttt ttcccgcgcc tggctctgcg
     1861 attgccaaca gataaagtga gcagggttcc ggcatgttag tgtgaggatg aacaacgtca
     1921 accaatgact gacaggagag tgagagcgtt gccagtacag tattaaaaat gagctccaga
     1981 ggaagttggg tacttgaata caagtcgtca atgagatacg caagatcact aggaaataat
     2041 ctgtaggtta catctgttgg gaagtggggg gatgtctgca aggcttgcac ataatcggga
     2101 ttcattgtaa tggacctcct tcacatactg taatcagcac aaagcttatt gttcagttca
     2161 gaaacatacc aacattgtga gtatgttcgc tgacgccaat aataaaatga ctgatctcca
     2221 ttcaccagtc ccggcgcatt tatggttctg acaaaagtaa acaacatggg agccagaact
     2281 ctgcatcgca gcgacaaact atggatgcat gaaaaacagg gaacattacg caccagagcg
     2341 ttgagagtct tcctccttgc ccaatacgaa agtcatctat ccacccgtct tcaatcttcg
     2401 ggatggttgt tttatgaaca gagtaaaagt ttgaagcaag ctgagaacct ctgtttccac
     2461 gtaaaggtcc gctatgaaga aaaatgggag tttgcatgct caactaattt acctgtaggt
     2521 gcttgttact gtagttattc aacaatccca caaatatcct gcatcagatt acgggctgtc
     2581 tggctaatgc cgccaagctg gaaaaaaccg tgaatgacac cgaggtaacg ctgggcggta
     2641 cactttactc cttgttgaat catacgggaa aacagttcct caccttcatc gcgtaatggg
     2701 tcatattccg ctgtgatgat atgaacaggt ggtaacccat tgaaatcttt tctgaataat
     2761 ggactggctt ccggatgttc taaatccaga ccggaaaaat aagcctcata accactaaga
     2821 aacgtgtctt ctgtcagcac ataatcactt ccgtttaatt tgtaactatc gcatgatgct
     2881 gttgcatcca gcatagggta aataagtatt aattttcgtg gtaaccactg agcgatgttt
     2941 ttcacccgca gtgctgttat cagtgcaaga tgtccccctg cgctatctcc cattaacgtt
     3001 attttgttgc gatcaacttt caacatttct gcgtgttgcc atacaatatt agcggcatgt
     3061 tcagcatcgt catgtgccgc aggataaata tgttccggcg ctaatcgata ttgtacagct
     3121 atgacacgac agccgctcag gaaagcaagc tgtcgaaact gattatcatg ggttacaaaa
     3181 ccaccactga caaaacagcc accatgataa tagacaattg cggatgacaa tttatcatcg
     3241 tttaaaggcg aaaagatacg caataaaata tcatcaataa tcacatcact gatgtttacg
     3301 cgggtttctg tatcgccggc cagagcagta cttgcaatat atcccgcccg acgttcatca
     3361 aatgtctgat cacgacagga tggacgacca cccgcaacaa aatcatttac aaggtttgcg
     3421 atttcctgtt caagtgccat acaaatcaac tccgaccgat aatatttaaa caacgctacg
     3481 ggaagtataa atactctgac caaaatataa caggtgaata tccatagctt caacaggcgt
     3541 aatcaataag ggcgtacgga gcttgcccgt tattgctgca ggatcgacag actggttttc
     3601 gtccttgcgc atgtaacagt acaaccacag agaaaagcat caatggacac tttatgttgc
     3661 gtcagggcag cgaacgactc cttttgttga cgacacatat caccttctgt actggcaatt
     3721 acggcattca gagtctccct gagttttaag ctcctgctct tacggaccag cgacaagcaa
     3781 taaataacta tgacaatgaa tctaataaca gcataaatat gatactcatc gtgattagta
     3841 taatcggggc agaattatat tcactgtctg atttattatt gggttaaata taattaaagt
     3901 gttctcaaaa gtctggctgg attaccggca tacacgcctt ttaccagcac gggctttgta
     3961 accacacttc cagcaccaat cactgctcca ctgcaaattt ttgttgttaa aatagtcgct
     4021 ccgctaccca ctgttacagt gttacctaat acgatacgta tccagctttc tgcagaggca
     4081 tcgggaccac cacacttaaa aagatcatta gcaaaagtta cgccatggcc gatgaaacag
     4141 ccatcaccta aaatgacgtt ctcacagatg aaactgtggg actgaattcg gctccggttg
     4201 ccaatgatgc atcccttctg tatttccaca aagggaccaa taaaaacatc atcccccagt
     4261 tcacattcat agaggtttac tggcataacg actttcacat tttcaccaaa tttcacatca
     4321 cgaatgccgc tgtcaataac gttcatatcc gttgtatcct ccctcaggat gattcttatc
     4381 agaacatttc acctgtcaga atattcccgg taatgtgata caagatccag tctcaccggg
     4441 taccacgtgt tattgcccgt atccttttgc ccgttacgag tctacaactg gttttgtatt
     4501 ttgcaatcca ggcctgttac accagagcat cctctcactt ccccctcact ggtcagtccg
     4561 ggattcaaca tcatcctcca gcactaaccc tataccagtc ctgaaccttc cctcgccagc
     4621 agcctactgt tcaccacaat cctcttccgt gaatggcagt aagtgagctg caccaaaata
     4681 aatcggtact tccgatgaca gacagtggaa gtgccaggag aaatatgaac atgagaatgg
     4741 aagcagataa aaataccgtt gcatgacttc aggacgggtt atcagaatac gagctaaggg
     4801 cgtcagccaa cgcccttacc gtggtataca ctcaggcacc ggataccatt ttaccgggtt
     4861 cacctgccat ccagtagaca cctcacccca cgactgcaac cataattacg ttgacctgaa
     4921 ccgtcatgca gcttcgtttc atttttcacg agataacggg ctctctccag ggctttctgc
     4981 attgcttcca catcaccacg cttggcccag agcacagcgg atccacccgg ccttttatca
     5041 gaatccgtcc gggtatcaga acatccacta tgcaaaggcg tgtcatcgga agaaggggaa
     5101 gcatcagccg ctgctggttt acgggaaggc gtccacctgg aaaaatggac caggccatcg
     5161 ccttgccagg gctcaggatg cagagcgctg caccatgcct gttggatcat cgtcgccagc
     5221 gaagacggtt cgctgaaatc cccgagcgag caccagatat ctttgttgag cagtaatatc
     5281 acgtgataat gtttcctgcc tttttccttg ccaaactccc gacaccagaa ataacgcagg
     5341 gtggttgcat gcacacgctg attggcacat ctttttttcc gctggtaagc ctgaattttg
     5401 gctttcaggg atgcaaaaaa acgagaaatc accgctgaat ccggatcggg atccatgatg
     5461 gtgacaggca tatactcagg gaaacgtaaa tccacccgga ttaacgagac ggcacggtgc
     5521 tgcgccgggg cactgtcgag aaggctgtca aaacgggata accagtaagc attgtgtatt
     5581 gattgattca tgatgagaga tctccgttga tttggattga cttcacatca ttcatcaacg
     5641 cggtgaaact ttacggcatc ccgccaatga gcaacgccaa tagctcctga ttaaaacgga
     5701 atgaatcgtc ccgtaacgaa aagataatcc tgcacacgac agcaaaacgt aaaacaacag
     5761 catgaatgag ctaaatgttt aaagaaaaaa tgtcttttca cattattttt cagcaagtaa
     5821 tcataccagc atccctaaca tacgaaatca catagtgatc gatcgctgat tcgcttaaca
     5881 actgatcgaa tacaccggaa tttacactaa catcctgatg ggtaacggaa gactaaaaaa
     5941 cctgtgttct attgattgca cggaatgcct gtgcactggc ctgaccagta agcacaattc
     6001 tacgagtagt aagatatcta cgccagaacc atgcggcagt agccaccata tgttgccagg
     6061 gtgaaaaata agctcagagc tataggggag taattcccgt taatgtcatc gctgtacctc
     6121 aatcagttca caaactggct gaaacgttaa gctgaagcct ttattcttca gatgtatttt
     6181 ccggtttacc tgtaaattat cttctgatat ttatcacatt agtagttgtg ctgattatct
     6241 acatacttca aggtctgaaa tatgaagcta acagaagaaa gtctgaatct agtcattgac
     6301 atcctgaatg atttgatctg tgaaggaaaa atgctgaacg atgtggagcg cagatctctg
     6361 ctcaatgcag ctatgacaat tacagcggta aaagaacgtt ctgtcgtatc tgtatcatcc
     6421 cgaaaagagc aaagtaagaa ggagaagaag gaaagagaga tcgatcctcg tttcccaaat
     6481 gctggtgtgg catggaatga ggcagacgag agtttattgc gagatgttct ggaggatgta
     6541 ccggacgatg aaattggtaa acaccttttc tggctggcag agaaactagg gcgaaccccg
     6601 tacagtgtag cctgtaaagt cagacaaatt aagaaattac ctgcagaatg gaaggatcaa
     6661 tttcgtaaaa tctctgacag gatccgtagt tctggaatgt cagtcagtga atatctacaa
     6721 cgccagggac tgttaagccc agagtcaggg gaagaaaaca atcctgtcag tcataacaac
     6781 aatgtagctt aatcctgaag aagatgaaca gtttatactc tttaaactgt tcccttaatt
     6841 ttagaaaaac agtttgtgta agtgcatgct ccaacattct gaaggagtta tttcttacaa
     6901 tacatcataa tcattggcag atcctcatct gaatcaaaac accacaacca atcatcacac
     6961 tttttctgaa aagagcctta tgatgaagat aatcacctta cgctgagaaa aaacaccagg
     7021 gccagttaat caccttatca atctcgccgt tacaaaatca tccataagcc atttaacagt
     7081 gttatcgcaa actcacagta aatcgcatca ggttacctca atcggatttg agttaattca
     7141 ctgggcttta cgtaaagata cttttctcag gttaacgact aaaacacgaa aactcagaaa
     7201 aggttaaaca caggttcagt aaaatcgaat atcatcttga ttgcttaaac aggacagagg
     7261 gttttatagt gaggttaact gattttagcg atcgtaaaaa
//
LOCUS       DAZGAP010000217         7271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGAP010000217 DAZGAP010000000
VERSION     DAZGAP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.218"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..918
                     /locus_tag="ACHLAM_004523"
     CDS             49..918
                     /locus_tag="ACHLAM_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7855955.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(968..1321)
                     /locus_tag="ACHLAM_004524"
     CDS             complement(968..1321)
                     /locus_tag="ACHLAM_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO7855956.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1459..3105)
                     /gene="groL"
                     /locus_tag="ACHLAM_004525"
     CDS             complement(1459..3105)
                     /gene="groL"
                     /locus_tag="ACHLAM_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO7855957.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3149..3442)
                     /locus_tag="ACHLAM_004526"
     CDS             complement(3149..3442)
                     /locus_tag="ACHLAM_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO7855958.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3718..4974
                     /gene="yjeH"
                     /locus_tag="ACHLAM_004527"
     CDS             3718..4974
                     /gene="yjeH"
                     /locus_tag="ACHLAM_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO7855959.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4990..5466)
                     /locus_tag="ACHLAM_004528"
     CDS             complement(4990..5466)
                     /locus_tag="ACHLAM_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO7855960.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5803..7239
                     /gene="aspA"
                     /locus_tag="ACHLAM_004529"
     CDS             5803..7239
                     /gene="aspA"
                     /locus_tag="ACHLAM_004529"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO7855961.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
BASE COUNT         1811 a         1853 c         1752 g         1855 t
ORIGIN      
        1 taaattaaaa ttaattattt aattgagaaa tggttaggga gaacctacat ggccataagc
       61 atcaagggtg ttaataccgg tgttattcgc aagagcaata atttcattgc actggcgctg
      121 aaaattaaag agcctcgcaa taaagaatcg ttgttctttc tgtctgtcat ggaactgcgc
      181 gaccttctga ttgcgctgga aagccgtctg catcaaaagc ataaactgga tgctgctgca
      241 cgccttcagt atgagcaagc tcgcgataaa gtcattaaga aaatggtaga aaatatccca
      301 gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa cacgctggaa
      361 cttaccgata atcagggcga aaatcttacc ttcgtattaa cactacatga cggtagcaaa
      421 tgtgagttag tcatcaatga attacagatt gaaatgctgg cgcgagcaat cattcatgca
      481 attaataatg cggaaatgcg tgaactggca ttacgtatta cttcactgtt agatttcctg
      541 ccgttatacg acgttgactg ccaggacaat ggcaatctgg aatatgacac ctattcccaa
      601 ccggaatgga aacataactt gttcaaccat tatctggcgg tgctttaccg ctttaaagat
      661 gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc aacgccgggt
      721 aaagaagtgg aggcgatcac tcgccgaatg cttgatttca gcccccgact gaaaaaactc
      781 gcgggtgtgc catgtcaggt ctatgttcgc accgtagcgg caaataacgc ccaaccgcta
      841 acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact
      901 gcaccacagg cgaaataatc tcatctgttt tactacgagg ctggaagtat ccagcctctg
      961 gatcacatca gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa
     1021 aactggacaa cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg
     1081 cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt
     1141 gttgcccgga aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg
     1201 cgcctggctg ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg
     1261 agctacaacc cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca
     1321 tgttattccc cataatcaat gagctggacg ccactcgcgt cgtccgtgtc tgaatcttat
     1381 actagaaaga tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt
     1441 ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc
     1501 agcagcgcct aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt
     1561 gatcatcagg ccagccacag aagctgcgta ctgcagagca gaacgagtta ctttggttgg
     1621 atccaggata cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta
     1681 gttgccgtcg ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcaa
     1741 tacgatctga cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg
     1801 gtcttcgttc tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc
     1861 accaccagca accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac
     1921 gcgtgctttt ttctctttca tttcaacttc ggtagcagca cccactttga taactgcaac
     1981 gccgcctgcc agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc
     2041 ttcttcaatc tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac
     2101 gccatcaatg atggtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc
     2161 ttccagggtt gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt
     2221 cagggttgcg atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac
     2281 cgcagcaact ttcacgatgc cacgcatggt gttaacaacc agagttgcca gcgcttcgcc
     2341 ttctacatct tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac
     2401 cggcagcatt tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc
     2461 cagttctact gcgccagttt ccggcttgtt gataaagtaa ggagacaggt agccacggtc
     2521 gaactgcata ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt
     2581 gataacgcct tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc
     2641 gtcggagtta gcggagatgg taccaacctg agcaatcgct ttagagtcag agcatggtac
     2701 ggacagcgct ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag
     2761 gtccatcggg ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc
     2821 cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc
     2881 tttcaccatc tgcgcaccca tgttttcgaa cttgtcttcc agttcgattt cacgagcaac
     2941 ggaaacacca tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg
     3001 gcctttcgga ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat
     3061 tttcacacga gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta
     3121 aattcgtatg ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt
     3181 cggacatgat caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt
     3241 tgaaaataac gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga
     3301 tacggccatt gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg
     3361 tcagaacgat gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat
     3421 catgcaatgg acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg
     3481 gtgacgccgg agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc
     3541 gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc
     3601 tgattcacct cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg
     3661 ctaccatcga aaatctacgc agttgcccaa aatttgggcg caatcggaca tcaacccatg
     3721 agtggactca aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta
     3781 ttaggcactg gcgtgtttgc cgttcctgcg ttagctgcac tggtagcggg caataacagc
     3841 ctgtgggcgt ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt
     3901 ctgggtcgcc actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt
     3961 tcgcggcttg agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct
     4021 gccgcactac aaattgccgc cgggttcggc caggcaatgt ttggctggca tagctggcaa
     4081 ctgttgttgg cagaactcgg tacgctggcg ttggtgtggt atatcggtac tcgcggtgcc
     4141 agttccagtg caaatctaca aaccgttatt gccggactta tcgtcgcgct gattgtcgct
     4201 atctggtggg cgggcgatat caaacctgcg aatatcccct tcccggcacc tggtaatatc
     4261 gaacttaccg ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggag
     4321 gcatttgccc atctcgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg
     4381 atgattggtc tgctgctggc aggattagtc tactggggat gtacggtagt cgtcttacac
     4441 ttcgacgcct atggtgaaaa aatggcggcg gcagcatcgc ttccaaaaat tgtagtgcag
     4501 ttgttcggtg taggagcgtt atggattgcc tgcgtgattg gctatctggc ctgctttgcc
     4561 agtctcaaca tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat
     4621 cctgaccact acctgtcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg
     4681 gtgctcggct gctgcgtggt gagcactttg gtgattcatg ctttagagat caatctggac
     4741 gctcttatta tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca
     4801 ggctgtaaat tattgcaagg acgttatcga ctactggcgg tggttggcgg gctgttatgc
     4861 gttctgttac tgacaatggt cggctggaaa agtctctatg cgctgatcat gctggcgggg
     4921 ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc
     4981 gtttcgacat taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt
     5041 tcgctggtac tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc
     5101 tggcatgcga gaaaagcgca aatgcggcat caacttcact gtcagatgct tttgcaccgg
     5161 cggcaacaaa agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa
     5221 accggcaatg atcagcgaaa cacttttaat catctccgcc gctgggtttt cacccgccgc
     5281 cattttttgc tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc
     5341 gataactgac gtgaatataa ccagcacgag agtcagcaat acccccaata catgggcaac
     5401 ctgaataaag attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca
     5461 gcgcaaaggt ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga
     5521 atcgcgttca gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt
     5581 ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc
     5641 gacacttaaa gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat
     5701 tcggtcgatg caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg
     5761 tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt
     5821 atcgaagaag atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac
     5881 actctgagag cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa
     5941 tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa
     6001 accattccta aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac
     6061 ggaaaatgca tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta
     6121 aacatgaaca ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa
     6181 ggtgaatatc agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac
     6241 gcctacccga ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg
     6301 attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa
     6361 atgggtcgta cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct
     6421 ttcagcatcc tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg
     6481 gaagttaacc ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct
     6541 ccgctggcag tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac
     6601 ctgatcgaag cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc
     6661 ctggctgtga agatgtccaa aatctgtaac gacctgcgct tgctctcttc aggcccacgt
     6721 gccggcctga acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct
     6781 aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac
     6841 gacaccactg ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggagccg
     6901 gtcattggcc aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg
     6961 ctggaaaaat gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac
     7021 aactctatcg gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc
     7081 gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt
     7141 ctgttgactg aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct
     7201 tacaaagcaa aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata
     7261 aaaaaggcac g
//
LOCUS       DAZGAP010000218         7161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGAP010000218 DAZGAP010000000
VERSION     DAZGAP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.219"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..455
                     /locus_tag="ACHLAM_004530"
     CDS             204..455
                     /locus_tag="ACHLAM_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7855962.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(486..680)
                     /locus_tag="ACHLAM_004531"
     CDS             complement(486..680)
                     /locus_tag="ACHLAM_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855963.1"
                     /translation="MTIAERLIQKGFDEGFKQGFKQGALEVAREIACRLRDIGWPPER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(742..1680)
                     /locus_tag="ACHLAM_004532"
     CDS             complement(742..1680)
                     /locus_tag="ACHLAM_004532"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7855964.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQYKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQKGRM
                     EEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            complement(1677..2066)
                     /locus_tag="ACHLAM_004533"
     CDS             complement(1677..2066)
                     /locus_tag="ACHLAM_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO7855965.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            complement(2256..2522)
                     /locus_tag="ACHLAM_004534"
     CDS             complement(2256..2522)
                     /locus_tag="ACHLAM_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855966.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(2615..3049)
                     /locus_tag="ACHLAM_004535"
     CDS             complement(2615..3049)
                     /locus_tag="ACHLAM_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO7855967.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(3779..4279)
                     /locus_tag="ACHLAM_004536"
     CDS             complement(3779..4279)
                     /locus_tag="ACHLAM_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO7855968.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(4740..5336)
                     /locus_tag="ACHLAM_004537"
     CDS             complement(4740..5336)
                     /locus_tag="ACHLAM_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855969.1"
                     /translation="MMKSDEKYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVRAAQDWLCDRKLVDITDILR"
     gene            complement(5333..6052)
                     /locus_tag="ACHLAM_004538"
     CDS             complement(5333..6052)
                     /locus_tag="ACHLAM_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7855970.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGELCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENTI"
     gene            complement(6049..6483)
                     /gene="psiB"
                     /locus_tag="ACHLAM_004539"
     CDS             complement(6049..6483)
                     /gene="psiB"
                     /locus_tag="ACHLAM_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7855971.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(6538..>7161)
                     /locus_tag="ACHLAM_004540"
     CDS             complement(6538..>7161)
                     /locus_tag="ACHLAM_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7855972.1"
                     /translation="MQQPDKSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDA
                     PSGKEGAAFMAMMAEKARLAALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQ
                     TRECGHTSRSPLDTLESAIGFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARD
                     AEKMKKGDAAEHAEFHMKDNRWVPGWMCTPRPQMDATEHTTNLADAA"
BASE COUNT         1630 a         2067 c         1910 g         1554 t
ORIGIN      
        1 gaagaaaaac gacggcacaa caatggcatg tcgtggtcac tggtgcacca ctcccgcaca
       61 ggtgaaaaca gagaaaacaa cacgacatat acaatcaaaa aaaagtgaaa tttcttcact
      121 ttttcactct tttttatcgc gctgacttgg tgactatgat gataaaataa agtgaagttt
      181 tttcactttt tcaaaggtgt tttatgaccg gctgggaact gcgaatctgg cgaaaaagca
      241 tgctctggtc acgcgaaaaa gcggccagag aatttggcgt gacacagaga acatggcacg
      301 cctgggaaaa tgcggaacag gtggatgtca ccgtctggag gaccacccag gcactttcag
      361 tccgtgacct gctgcctcac atgcagggga tgcgtaaggc agatatcatc aggcgactgg
      421 aaaatgaact gggggaaacg gcagaggatg tgtgagcgtg attcacagcc tggctgaatg
      481 ccaggctact gctcatcagg aaacagcttt ttcagttctt caccggaaag tccggttgcc
      541 tcctgaatcc gttccggcgg ccagcctata tcccgaagcc ggcaggctat ttcccgcgct
      601 acttcaagag ccccttgttt aaaaccttgt ttaaaaccct catcgaaacc tttctgtata
      661 aggcgttctg caatcgtcat gctgtttgct ctgctcctgc tgacaaaaaa cccggcattt
      721 tgccgggctt tgtggttctc atcagtgagt gatgttcttc agttcagctt cggaaagtcc
      781 ggtgatctga cagatttgtg cagatggcat tcccgttttc tgaagctgac gcgcaatatc
      841 ctgcttctcc tccatgcggc ctttctgtat tccctgctgt attccctgct gtattccctg
      901 ctgtattcct ttctgtattc cttgctgctc aagacgttcg gcaatagtca tcagtttatc
      961 ctcatgctgc ggcagacgct cagccagcgc ccgcataaat tccggtgata cagagtcccc
     1021 tgcgttaacg atgtaatgaa tcagaacacg cacctgttca tctgaaattt ccaccatgac
     1081 ctgcgggagt ttgtccagca gctccatcat gtcacgatgg cggatatgct tcatcagcag
     1141 cgtcagtgcg gccatccggc gatggttcat gatttcattg tcatcaacga cagtgatatc
     1201 aaccagcgga aacggctttg tgtatatttt agccgcaagc gccggatcct caaaacagtc
     1261 cagccagttc atgctgtacg ggtacgggct gcgctcacca tgatagaaca gcaccggaat
     1321 caccagtggc aatgttttgt actgctccag atgtctctgc atcgccgcca cactgtaacg
     1381 taacatgcgg aaaggcatgt acagattttc ggtgctctgg tgctcgatga gcgtataaac
     1441 atagccgtct ttacccccgg tggttttcac actccagagg atatcgctgg cgtactgatg
     1501 caggtccggc tcaacaaagg aggcgggttc cagcttcagc gtggacaggt cgcacaactg
     1561 ccggtactcc gccggaagat gcagttccag aaaatctctg gcgacgtcgg ggttcgccag
     1621 gaacgaccgg aacgcggcat catgcggcgt gggcgttgtg gtgtttttct tcttactcac
     1681 ggttcctcca tcaaatcagc agcatcgcag caatcatcgt ccaaatcccc acaaccaaga
     1741 ggacagcaat tgcgatcaaa gccgtgacaa caatagccac gttctgcgtc acggcgtatt
     1801 tcttctgcaa tctcttcagc ggaaaggtaa tcaaactcct cacgggccag ttccaggacc
     1861 agcagatgtt gttctgtcgg caatgtagcc acaaaattat caatagcagc actcaaacgt
     1921 cgctgatata gctcataaac aagaccacaa cggtgcatta gttcttcgga taatagttcc
     1981 agttttgagc gaacgctgtc agcattgaaa gtgacttcat cagatgacat ctgaaaatct
     2041 ttgtcagtgt caaaacaaga cttcattttt atctcctgcc cgtccgggcc ctgaatgaaa
     2101 gagaacctgt ttcccttctc tgttttttat ggaacaggag gaatccggta ccatgcgaca
     2161 tagacgtcat gatgtttttc atcttgctca cggttcctcc tggtaaagta cctgaacata
     2221 tcctgccacc tgcacagaca gacggcaggg ggtaatcagt ggcagacccg ttcaatttca
     2281 agcgcacgga gcaggtgccc ccactcgctg actttcatgg cggggatacg cacccgcttc
     2341 cgctcactgc agcgtctggc gatatgaccg cccatcgcca ggacgtccgg gttaatctca
     2401 tccggtgcca gttcctggcg tctgaagcgg gtatggcggt gcaggggttt gttctcccgc
     2461 aggcactctt cacgcagacg ctttacatac tcggcggttg cgaagataat tgatggtctc
     2521 atggcataat ctccatagtt agtgaatgag atgccggact tccgtacggg gaagcgccgg
     2581 cggagaaagg cagggtttat cgccctgctt tttttcattc cggacagcga cgcagtgcca
     2641 gcgcgagcgc tttcatcttc cagccttccg gcgtgcggta catcaccttc cagtagccgt
     2701 cgatgaacac ataatggaag cgcacctctt ctgtgctttc cgtggcaata gcctccatga
     2761 aagaacggta ctcacgcgcc tcactgtcca tccggaaacc actgtcacgt ccgtaataag
     2821 ccgtcacacc gtccaccggg ttctgaagcg tatgcccggc gggtttgtcg caacggggac
     2881 cgaggcaacg gatatcaccg tgttcaacca gggcttcggc ctgctgaccg gtggcatagt
     2941 gggtatgcag gatgcgaccg ttccaggtcg gctcaccgtg caggtggcag taaacggtac
     3001 ggaacacccc gtcgcggcaa aaaacggaaa tcgtgctgct gactgtcatg aaaacgtctc
     3061 cttctgctga atcaggtcgc cgggccgacc ggttgctccc ccggaggggc gaaagcccct
     3121 tctgccgtcc acttcttcgg ggtttctccc cgcagtggac ctttccgatg gggcagcgac
     3181 agccacggca cggggcagtt gtccgggggc agacgcaaaa gtttttgacg cttgcggcaa
     3241 aaagttttgt ggctggtaca tctccccctg gacggctgac acgtgttgtg gccgcttcag
     3301 cccccggaaa gtccacagcg ggaaacaggc cgcaggggtg gcggcggaag ggcagacaac
     3361 tgcgccatca ggcgcagttt ccttacacac cgtcaggtgt gcagggagcg cagatcacgg
     3421 cgcgacagag gcggcgcgac cggaacggtc gctgcaagcc aggtttttca gacatcggac
     3481 cgtagcggca gcccgaaacc gcgccagcgg gttcggcgga gactggcggg tgcgcgagca
     3541 gctgcctgtc ggagtcgggc ctgacgaccg gatgccgcca ggcagccggg agccgaaggt
     3601 gtccccgcct ccggaaatca gaacaacaga aacgacataa cagaaaaatg cctcactcca
     3661 tgacgggcag gaagggcagc gccgcaggcg cggccctcct gcatttcagg caggcgtaag
     3721 cctgccaggg ttttccgggg gcctggcagg ccataccccc gccggagcgg ggagtaaatc
     3781 accgacggaa cacatgaccg tcaataaagg tgaaggagtc caggaataaa tcccgcgcat
     3841 acgcctcata gtcaaaatag agcgccaccg tctccggcac gtcagccagc aggccggtat
     3901 cactgacgaa ctcaaccgca aaagcctcct cactgtcagc ctcgccccac caggcatcgc
     3961 ggaagctgtc aaaatccgtc tcaccggtat catccaccca gagacgataa gcctcttcgc
     4021 agccttcatc ccgtgcctgg cggaagcctt caacccaggc ccagttgata tgacattcag
     4081 aggccatatt ccccgggaat ccctcataat cctgaaacat cagttcagga tcggcttcat
     4141 cctggtgaag agcacggcag gcggcgaaaa agtcgcgctc atcatcaaac gtggtcaggt
     4201 caaaccagcg tccggcgatg cttccacagt tgtatttgtg ccaggttcca acgtatacag
     4261 caggtgcaac aacagacatt cccgaatcct ccatagtcac atgttctgcg gcaccgggcg
     4321 gtgccgtgcc tccataaaag ccgggcgata aggcggggat gcacagggcg caagggaagc
     4381 cgcagcctga cgcacacggc tggcgggagt tggagggtga gaccgcgaaa tctgagcggg
     4441 tgaacgaccg gaaaatcacg gcagacggga aggagggacc gggtgaccgc cgcgtgcatt
     4501 ttacccttgc atccccgcca gccggctaag atggaggcac ggcacagccc gcacacgacg
     4561 ctgaggccac ataactgaaa acccacaagg ccatgccccg catggccggg taaaagcgga
     4621 gccgccggcg acttccttat tccggcgagt gcagaggaca cgccgcatga cctccagacc
     4681 acccctgata tgaaacgatc atgatcggcg tgtccctgcg aaaaatatac atgcctggtt
     4741 caccggagaa tatctgtgat atccacaagt tttctgtcgc acagccagtc ctgcgccgca
     4801 cgcactacgc tactgtccgg tttcaccgct ttcagagaag atgccagtcg tttgatcagc
     4861 atcaccagtt cccgtgtctg tatgtccggc attgtctccg gcggagcaga gggactctca
     4921 tttccgacgc ccatcccatg cgcttccagt gtggcacgga caaaccaggc agcatcgacc
     4981 agttgctggc gaatccaggc cagatcggca tttctgtcac gggctgccat tcgctcaccc
     5041 gacagcgcct tgctgatatg gctgcgcacc agattatcca ggagttcctg cgcttcttcc
     5101 ggtgaaaagc cacctggtat ctctgtgcgc aacagtcctt tcctgtacag tagctgcagc
     5161 agatcaatct gcgaccgcac tgcaactatc agtacataac ggacaatatt cgcactggcg
     5221 gtggtttctg tatcattcag cagttcctcc ggatcattac caccggtatt gcagagcaac
     5281 ggcaacagag gtcgcatcca cacgggaacc tgatattttt cgtctgattt catcatattg
     5341 tgttctccag tttgttcggg atcatccact gctcaagtgc acgtacctgt accggtgcac
     5401 cacgaccggc ctcaccggct tcaaaaatca gccgccagag tggttcctcc tgccagaaaa
     5461 accggtcaaa tgatgacagt gacggaaaac gcccgcacca cccccagaaa atcgtttcca
     5521 gatcatgctc ctcaacaaca ccagaccagc gggacaacca gcttccgacc gtggcgggag
     5581 tatgaaacgc aaggtcaatc tgcgcctgag caaaacatgt ctggtattcc agttcccgtc
     5641 gttctgcctc acgttcccgc tggcggctgt atttctgctc tgtcagcgtg gttttctgac
     5701 gacgccgctc accttcacga aaaggaacgg acgggaacag acggtgcccc gcatcggatg
     5761 gcaggggaag aacacagagt tcaccaaaac tttcaatcag tttatccacg gcagccagcc
     5821 acaggagacg gttgccatga aattcctcct tcgtcagcgc aggagcaaaa aagcggagat
     5881 cggtcagcga aatccggcga ctgccattca ggcagcgaaa gaaagcgctg gcataaggcc
     5941 atgctgaaag tgtgcgtccc tgacgacgac gttgttcggt gacagccaca gcctgcatgg
     6001 cggcctgttg ttctgcgctg agggggatca gtgcccgtga gcgtgcgctc atgcctgacc
     6061 tccttctgcc atcaggatat ggatgatgtc gttaacactg tacccctgcg catcaagtgt
     6121 cgcggcaagc gccagcgtgt gactgatagc gacaggcgtg aagatatgtt gcacctgaac
     6181 gacggcgaaa ggctgtccac cacaattcac cagaaccagc atccagagct gagagacgtc
     6241 tcccggcgaa cataatgcca ggtggaaacg ttcgtgggct ggcgtgaaac gaacctggac
     6301 ggggaaaaag cccccgaact cactgccata ctcgccattc atcatccagt tagccggtgc
     6361 gtccacttca cgcatcacct cgtgagtgag attacggcgc atatcgcttc cggcagcccg
     6421 gatatcttca tattcctgtg cgttcatgga ctgcagggca ttcagggtca gttctgtttt
     6481 catcacggta tctcccttgc tgccgccccg tctccggcgg ggcggtgtgg ttgttcatca
     6541 ggcggcatca gccaggttag tggtgtgttc agtggcatcc atctgtggac gtggtgtaca
     6601 catccagcca ggcacccagc ggttgtcttt catatggaac tctgcatgtt cagccgcatc
     6661 gcctttcttc attttctccg cgtcccgcgc agcaccggac agtccggcat cattcagggc
     6721 tgcgataatc tgcggctttt tcaggtgtcc gaagaagttt gcttttgtcg gctgccacca
     6781 gtcgcgcatg tgaaaaccga tggcgctctc cagcgtgtcc agcgggctgc gtgacgtgtg
     6841 accacactcg cgggtctgaa cgccgtggat gctgcacgcg gtgcagaaac tgagcaggga
     6901 taacagcacc tcctgactga gtgaaaggaa cgtcgtcatg tcccgtgacc atccctccgg
     6961 taacagggcc gcaagacggg ctttttctgc catcatcgcc atgaacgcgg caccttcctt
     7021 ccctgacggc gcatcgctgg tcagcgaata atgtttacat tccaggctga tttgtgctgg
     7081 tttactgtac gctccgctgc caaacacatt caggcagagc gtccatgcca gcagtgccag
     7141 agatttgtcc ggttgctgca t
//
LOCUS       DAZGAP010000219         7128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGAP010000219 DAZGAP010000000
VERSION     DAZGAP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.220"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..149
                     /locus_tag="ACHLAM_004541"
     CDS             <1..149
                     /locus_tag="ACHLAM_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFO7855973.1"
                     /translation="VCGTCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRL
                     VLDL"
     gene            complement(196..1311)
                     /gene="rnd"
                     /locus_tag="ACHLAM_004542"
     CDS             complement(196..1311)
                     /gene="rnd"
                     /locus_tag="ACHLAM_004542"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO7855974.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(1393..3078)
                     /gene="fadD"
                     /locus_tag="ACHLAM_004543"
     CDS             complement(1393..3078)
                     /gene="fadD"
                     /locus_tag="ACHLAM_004543"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO7855975.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDSEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3283..3864)
                     /gene="yeaY"
                     /locus_tag="ACHLAM_004544"
     CDS             complement(3283..3864)
                     /gene="yeaY"
                     /locus_tag="ACHLAM_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO7855976.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(3903..4598)
                     /gene="tsaB"
                     /locus_tag="ACHLAM_004545"
     CDS             complement(3903..4598)
                     /gene="tsaB"
                     /locus_tag="ACHLAM_004545"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO7855977.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(4656..6566)
                     /gene="yoaA"
                     /locus_tag="ACHLAM_004546"
     CDS             complement(4656..6566)
                     /gene="yoaA"
                     /locus_tag="ACHLAM_004546"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO7855978.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            6698..7042
                     /gene="yoaB"
                     /locus_tag="ACHLAM_004547"
     CDS             6698..7042
                     /gene="yoaB"
                     /locus_tag="ACHLAM_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7855979.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         1700 a         1975 c         1789 g         1664 t
ORIGIN      
        1 gggtatgcgg aacctgcgaa acggcaatac tggaaggtga agctgaccat cgggatcagt
       61 attttagcga tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgcgcga
      121 agggtaaacg cctggtgttg gatttgtagt ttgccgaagc cggactgagc gcgcccagtc
      181 cggcttcgga agattttact gcggatattc ctgcaataaa ttgtgtaatg cttccgccat
      241 cagctcacca cgccagccgg aaatcagctc cggcaaattg ttctgcggtt tcagtttcca
      301 gtgccagttc agcagttggt tgatttgccg acgcgatgcc agcaattcgg cgctgatctt
      361 gtgcgtttcg ctcacgtcag taatcagcga cttaatcgct ttaaacgctt tacgataacc
      421 cggcatgtcc atcaggttaa gcatcggctg cggtaaggcc tcttccggca atgcctgcgc
      481 tttttccacc agcgccagca gcgttttacc gtgaaagcgg atttcgctac cggataagcc
      541 caggctgtcc agttcgccta aacttcccgg catataacgc gctacggacc acaaatgctc
      601 ttcacgcacg acaaagttca ctgccagatc gcgctcccgc gccttgcgca gtcgccagtc
      661 ggctaacagt tgcagacagg ccagttggcg tgtgcgtaac tgccaggcat tggtgatatc
      721 acgccaggca tcttccggag caacgacttc ctgacgacgc atttgcatca ggcggcattc
      781 atccagcgcc gcaggtagcc agccggaggc ctccgtttct accataagct ttgcggtgat
      841 cggtaacaga taccagacat ccgccgctgc gtattcacac tgacgttcgg tcagcggtct
      901 ggccagccag tcggtgcgcg attcactctt gtccagcgta acgccggaat actcttccac
      961 catggaagcg aaaccccatg acatcgggcg tccgcagaag gcagcaagga tttgcgtgtc
     1021 aatcaagggt tgtggtaatt cgccaaagac attgaggaac acttccagat cttcactgcc
     1081 cgcatggaga aattttgtga tggacggatc gcgcaggatc gctttcagcg gtgaccagtc
     1141 agagatcccg aggggatcaa ttagcgccag atgctcacca tcaaaaagtt gaattagacc
     1201 cagttgcggg taataggtgc gcgtacgaac aaattcagta tccagggcta tcgccggaaa
     1261 ggcacggacg gcttcacaca aagaagccag cgcatcgtcc gtggtaatca tttggtaatt
     1321 caaattgtat tctctttagt gggcgtcaaa aaaaacgccg gattaaccgg cgtctgacga
     1381 ctgacttaac gctcaggctt tattgtccac tttgccgcgc gcttcgtcac gtaattctcg
     1441 tcgcaaaatt tttccgacgt tagatttcgg taactcatca cgaaactcca ccagcttcgg
     1501 tactttgtag cccgtgagct gacggcggca aaaagtcacc agtgactctt cggtaagcga
     1561 tggatctttt ttcacaacga agattttcac cgcttcacca ctggagccgg aaggtacgcc
     1621 aacagccgcg acttcctgta cgccaggatg ctgcatgacg acatcttcaa tctcgttggg
     1681 atagacgtta aaaccggaaa ccagaatcat gtctttttta cgatcgacaa tgcgcaggaa
     1741 tccttcttca tccatcaccg cgatgtcgcc ggtgtgtaac cagccatttt tgatgatttc
     1801 atcggtagca tccggacgct gccagtaacc cagcatcacc tgcggtcctt tgacacaaag
     1861 ctcacccggt tgaccaggtg gtacttcact atcatcatca tccaccagtt tggcttccgt
     1921 cgacggcacc ggcaaaccga tgctaccact atgataatca atatcatacg ggttaacgct
     1981 caccagcggc gcacactcgg tcaggccata accttccagc agatactgtc cagtcagttt
     2041 aacccaacgt tctgccacca cttgctgaac aggcatccct ccgcctgcgg aaagatgcag
     2101 actggagaaa tccagctgct ggaactcttt attgttcagc aacgcattga acaaggtgtt
     2161 aacgccagtg atagcggtaa acggatattt cgctaactct tttaccaacc ctggaatatc
     2221 gcgcgggtta gtgataagca ggttctgccc ccccagttcg ataaacagca ggcagttaat
     2281 ggtcagtgca aaaatgtgat acagcggcag cgccgtcacc accagctctt tgcccggatg
     2341 caacagcgga ccataggtcg cgttaacctg ttccaggttc gccagcatat tgcggtgagt
     2401 cagcatcgcg cctttcgcca caccagtggt gccgccggtg tattgcagga aagctaaatc
     2461 ttccggcacc agttcgggtt tgacgtactg catccggtag ccgttgtgca gggcgctacg
     2521 aaatgaaatg gcatctggca gatggtattt cggcaccaga cgcttgatgt atttaacaac
     2581 gaaattgact accgtgcctt ttgccgtaga tagctgatcg cccatacggg tcagaattac
     2641 gtgctgaacg gcggttttat caaccacttt ttccagagtg tgggcaaagt tagacacgat
     2701 aacaatcgcc gatgcgccgc tatcgttaag ctgatgctca agttcacgcg gggtatacaa
     2761 cgggttaacg tttacgacaa tcatcccggc acgcaaaatg ccaaacagcg ccaccggata
     2821 ttgcaataaa ttaggcatca tcaacgcaac gcgatcgcct ttcttcagtc ccaacccttg
     2881 ttgcaaataa gcggcaaacg cgcgactgcg ttcttccagt ttgcggaagg tcatcacctc
     2941 ccccatattc acaaacgcgg gttgatcggc gtagcgcgcg accgactgct caaacatatc
     3001 taccagagat tgataacggt cagggttgat ctccgtcggg acgtccgcgg gataacggtt
     3061 aagccaaacc ttcttcaatt cttcacctct aaaatgcgtg ttcgtcgtca tcgcaacccc
     3121 aaatgatata catgctgtta acataatatt aactcatcat accagcttga taattaccca
     3181 acgaaaaggt tgcgaagcgc gtcactattt atttttatct ttaccgtaag aatgcagaaa
     3241 cagcggacca gccgctgttt ctttttcttt aaaaacaagc aattattcag ttacaacagt
     3301 ttgtactctc gcggggccgg gattatacca gccccatcca ccgtagccat agggccagcc
     3361 acgaccgcca taaaaccatg gatcaatcgg ctgaggcggc ataatcacct gctgggttaa
     3421 atgccaacgt ttgtaacccg ttacctgcat caccataaat ttatagggcg tgttgccgat
     3481 tttgccgtca accgcaccgg tgattggccc aactaccgta accagttgtc cacggaaatc
     3541 taccgggtcc agaaaaccgt tcacatcagc ataaatgcga ccgcgagaag gttctcccag
     3601 cgtcggtctg gctccgctgt ccagtggtac ggtggcaatt tccaggcggg ttttcccttg
     3661 ctggttttgt accgcaacca ctttgccacc aaagcgtgcc tcctgaccaa cgtacagctg
     3721 cggcgcactc atcacccgaa ctaaatcttg ttgcggcgtg ggactgctgc ctttaatggc
     3781 gtccggcaca gtaacacaac cgctgagcat tagcgcaaac gtacctgcca gtatgccttt
     3841 gataacattc ttttgaaccg ccatgatgcg actccttttt tctcagggca tactcttaag
     3901 attcattctt tgcccggaag tttcttccat gcgacgttgt tacgtaaata aaccggttcg
     3961 gcatgttcca ccgccaccgt tttaccctcg gcaaacatct gacacgcaat cggaagcata
     4021 tcttctgcag cgggcagtaa cacttcgcca tcgcacaggg tcaaaccgct ctctttaccg
     4081 agatccggcc aggcttgcca gcctgtacct accgtcaccc attccccgga aagctgttgc
     4141 atccgttcat gaacgagttc gggtttgagt acggcttcgg tttcttcacc gtgccagata
     4201 cccttttcat cacgctgata ttcggcccag taaacttcgc ccatccgcgc atcaatagca
     4261 gccagcacgc gggttgcgcc gtttttgcgc cacgcccctt gcgccattgt catgagcgtg
     4321 gaaacgccaa tcatcggcag atccgcgcca agcgccagcc cctgcgcaat accaatacca
     4381 atgcgcacgc cggtaaagct accggggccg cgcccgtaag ccagagcgtt aatatcagtc
     4441 agggaagttc cgctggtggt caggatatcc tgcaccatcg gtaagattcg ttgagtatgt
     4501 tcacgagggc aaagctcaaa atgagcgttg acagtaccgt cgttccacag ggcgacagag
     4561 caggcctctg tcgcggtatc gatagccaga attcgcatgg gtcttcgtgc ttagatcaat
     4621 aaaaaggcgc gcatcatacc atactccgta acaaattacc tggaggatgg tatcgcaagg
     4681 aaacgaaccg cacgggcaat gtcacgggtg cgcggcgctg gcggcagact ggcgagaaac
     4741 gtcgcgccgt aagggcgcat caccagccga ttatcgcaaa tcaccagcac gccgcgatcg
     4801 tcggcgtcgc gaatcagtcg ccctactccc tgcttgagag taatcaccgc atccggcagt
     4861 tgtacttcat cgaacgggtc accaccacgc aaacggcaat cttccatgcg cgcttttaat
     4921 agtggatcat ccggtgaggt aaacggcaac ttgtcgataa tcaccaatga caatgtatcg
     4981 ccacgcacat ccaccccttc ccagaagctg ctggttgcca caagaagcgc gttaccggcg
     5041 ctgacaaatt gctgcaacag ttgccctttg ctggtttccc cctgcaacaa aacgggaagc
     5101 gtcatggtag cgcggaactg ctcagccaga tcgcgcatca tggcgtgcga ggtacaaagc
     5161 ataaaacaac gaccgttgtt agcttcgatg atcggtcgca acattgccgc cagttgccgt
     5221 gcggagcccg gttgattggt ttgcggcagg ttgcgcggca cacagagtaa cgcctggcgg
     5281 ctgtaatcaa acgggctggg taacagcaac gactctgcct gttcaatgcc aagccgcgag
     5341 gtaaaatgat gcagatcgtc gttcaccgac agcgttgctg aggtaaagat ccagctaccg
     5401 ggtttttgcg ccattaactc tttgaattta tccgccacgc tgagcggcgt gagcgccaga
     5461 gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc ccggctgatt gatctctttt
     5521 agccgtttca gccgcgtacg atacaacgtg gcgcgctcaa atgccgcatc cagcaaggcg
     5581 gaacgcccca gcgacagttt cgccacgtca taacaaagtt ccagggtgtc atcgagcagt
     5641 aaaaacgccc gttgaatttg cggattagct aacagttcgc gcaggttgcc acgataacca
     5701 ggctcaccga gttgcagacg aaaatcctgc gcgctctggg caaggcggtc ggcgcacttt
     5761 tgtaactgct gagtatcttt taattcggtg cgataggcga tggtgatgtc ttttgccagg
     5821 tcgagtagtt gtcgactgga gagtgactga ccaaaatact ggctggcaat gtcaggcagc
     5881 tggtgggctt cgtcgaagat catgacgtca gcttccggga tcagttcgcc aaatccactc
     5941 tctttcacca ccatatccgc cagaaagaga tgatggttta ccaccaccac atcggcgtcc
     6001 atcgcttttt tgcgtgcttt gaccacaaag caatctttat acatcgggca gtcgctgcca
     6061 aggcagttat cgttggtgct ggtgaccaac ggccacgcct gtgaatcttc cgccacgctg
     6121 acgcaggtgc tgatatcacc atcgactgtt tgattagacc aggagcgcag caggatcaca
     6181 tcgcttaaga tttgtaccgg cagatcgccc cccgccagcg cctgctgttc gagacgttcg
     6241 aggcagaggt agtttgagcg ccctttcagc aacgccacgt tgcccgtata tttcaacgcc
     6301 tttgaaaccg ttggcaaatc gcggctgtag agctgatcct gcaacgcttt tgagccggtc
     6361 gagataatga ctttcttttt cgcccgcagc gcaggggcca ggtaagcgta ggttttgccc
     6421 gtaccggttc cggcttccac caccagcggc tggccttttt ctatcgcctg ggtgacggct
     6481 accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg gtatcgcttt cgccagctga
     6541 ccgtctggtg caaaatcgtc cgtcacacta ccccctgttg atttgaacag ggattatgtc
     6601 aggatgaggg cgctttcgcc agttgaagtg gtgacggcga cctcacattg tggcagtctt
     6661 tgcagcacga aaatggaagt ataatgagga caaaatgatg actatcgttc gtatcgatgc
     6721 tgaagcccgc tggtctgatg tagtaatcca caacaacacg ctctactaca ctggtgtacc
     6781 ggaaaacctc gacgccgatg cctttgagca aaccgccaac acgctggcgc agattgacgc
     6841 cgtgctggaa aaacagggca gcaataaatc gagcattctg gatgccacca ttttcctggc
     6901 cgataaaaac gacttcgcgg cgatgaataa agcgtgggat gcctgggttg tcgcgggtca
     6961 tgcgccggtg cgctgcacgg tacaagcggg tttgatgaac ccgaagtata aagttgaaat
     7021 taagattgtg gctgcggtgt aagctttatc gaagcaaaat aagtcagacg ataatttatc
     7081 gacaatactg gtcggttttg cataaatcga ccagagatta agatatta
//
LOCUS       DAZGAP010000220         7116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGAP010000220 DAZGAP010000000
VERSION     DAZGAP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.221"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..576
                     /locus_tag="ACHLAM_004548"
     CDS             <1..576
                     /locus_tag="ACHLAM_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO7855980.1"
                     /translation="HLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQGKVVFIGV
                     GKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGETTEILAT
                     LPSLKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGDA
                     VALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            647..1594
                     /locus_tag="ACHLAM_004549"
     CDS             647..1594
                     /locus_tag="ACHLAM_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO7855981.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENHFNLIIKNNVEHLVKSEST
                     VLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGIDNIDLHTARQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1896..3272)
                     /locus_tag="ACHLAM_004550"
     CDS             complement(1896..3272)
                     /locus_tag="ACHLAM_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO7855982.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3272..3700)
                     /gene="tssE"
                     /locus_tag="ACHLAM_004551"
     CDS             complement(3272..3700)
                     /gene="tssE"
                     /locus_tag="ACHLAM_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7855983.1"
                     /translation="MATPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHSLMRQIEETLLRYEPRLNQIQVELLPQPGPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3704..4240)
                     /gene="tssJ"
                     /locus_tag="ACHLAM_004552"
     CDS             complement(3704..4240)
                     /gene="tssJ"
                     /locus_tag="ACHLAM_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7855984.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4221..5300)
                     /gene="tssG"
                     /locus_tag="ACHLAM_004553"
     CDS             complement(4221..5300)
                     /gene="tssG"
                     /locus_tag="ACHLAM_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7855985.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5264..7024)
                     /gene="tssF"
                     /locus_tag="ACHLAM_004554"
     CDS             complement(5264..7024)
                     /gene="tssF"
                     /locus_tag="ACHLAM_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7855986.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSNFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQERRVLDAHFS
                     PMGFDDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPEGMSGFEIDV
                     VLSENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDSDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLSAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSTGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1677 a         1853 c         1869 g         1717 t
ORIGIN      
        1 catctcatta aacattttat gcgtaacgaa cataaagcag ttgaagaagt tattgattcc
       61 cccctttcgg aggtcgcaaa tttaattaaa gtattacagt cttgtcaggg gaaagttgtt
      121 tttattggtg tcggtaagtc cggtattatt gcccgaaaac tcgccgcaac atttgccagc
      181 actggaactc cctcgttttt tgttcacggt actgaagccg tacacggcga tcttgggatg
      241 gtggcgaaag atgatgttgt tattcttatt tcaaacagtg gtgagacgac agaaatcctg
      301 gcaacactac ctagtctgaa aaaaatgggt aattatttga tctcctttac ccgcagtcat
      361 cgctcatcgc ttgcgataag ttgtgattta tccgttgaaa ttcccgtaaa aagtgaggcg
      421 gataatttag gtttagcgcc gtcatgctca tcaacagtcg ttctggttgt tggtgatgcg
      481 gtggcgcttg cgctctccga attgaaaaag tttactcgtg ctgatttcgg cttatatcat
      541 ccaggaggtg cgctcggtat taaagcaaac tcataatttt agaatataaa acagtaaccg
      601 gtcacgccat cttttggggt ggcgttcatt gatgtgagtg tcagctatga aaaacgttct
      661 ggtcaccgtt cccagctttt cagcgcgttg tgtttctgcc agcaagctgc taagagaaaa
      721 tcattttaac cttattatca agaataatgt ggagcatctg gttaaatcag aatccacagt
      781 attacgagaa tctatttgcg cagtcattgc cggaaaagat tgttatcaag cggatacgtt
      841 atcgcgacta ccaggagtga aaataatatc gagattcggg acagggattg ataatattga
      901 tcttcatacg gctcgacaat caggtattgt ggtcaataat gctgttggga ttaattcaaa
      961 tgcagtcgcg gaatttatca tcggtcttat ttttgcgagc atgagaaata tccccggtag
     1021 ctatcatgca atgcaaaatg gttattgggg tgagtcgcat ggctgcgagt tacaaggtaa
     1081 acgtattggc ctgcttggct acggcaatat tggcaaaaca ctggcgaaaa ggttatctgg
     1141 ttttgatgtt gagcttttag cttttgacaa acaacccgac tatcaggttg ctgacaaagc
     1201 tggagttcag tttgtatcaa ttgaagatat ctttatgcag tcgcatgtca tcattgttct
     1261 tttgcctttt tcttctgagc tggagaactt cattagtcat aaatatttgt cgatgatgcg
     1321 caatggtgct ctgatcatca atgccgcaag gggaaagttg ctggatgagg gcgctttact
     1381 tcaggtgatt gaagaacgaa atgtgtttgc ggcgctggat gtgtttagct ctgaaccgtt
     1441 ggcgcaattt agcccactgc tacatgccaa aaatatcatt acaacgccgc atatcgcggc
     1501 cgccaccgtt gagtcatacc agcaaaccgg gatacacgtt gcccagtcga taattgatta
     1561 cttcgcagga agggagataa aaaacgtgtt gtgagttcag cgaacaaaga ttttaaatac
     1621 aaaaaaaccg caatttgtag aacacattgc ggtttgtcag cattctaata aattggttgc
     1681 gggggccaca gtcaagaggt taatccaatc ttttgaaact taaggtattc actgttcccg
     1741 aacccaagct atctaccgaa atatacactc attatctgat gtgaatcaac gagtagatcg
     1801 aatggcctgt tcctctcttt ttttgtacac agagactcca ttttaaaaaa tggcaaggtc
     1861 acatatccct tattggtaca taaatccccc gatgtttact gactttgttc caccttcgta
     1921 tccagtaaaa tgacatagcg attcaacaac tgatttaacc gggtatcaat ttgcgtcaac
     1981 agcgttggcg aaacctctcc ggtctgtttt tgctcttcca gttgccgaag cagttcttcc
     2041 agcggcggct cctttaacga ctgccggatg ccgtacaccg ccgtttttag ttccgaaccg
     2101 gtcagatatc tccccttacg ttcatccagt gcatccagct gcgccgacag gcgctgtagc
     2161 tggctctgcg cctgagcata gccattcagt gcctcagcag acagggcgct cagctcccgc
     2221 tgtttgcgcc actgcgcgtt cagcgccctg acctgaggat tatccggcca cagggcatca
     2281 agctggcgta gctggcgata tccctgctcc agtggccagc gggcaggcaa tttgtttaac
     2341 tgctccagtt gcatctgact tgccgcaatt gttctgtcct gagccagcag cgccttatcc
     2401 tttacatcca gctttgccag ttcgccgctc tccagcgact gcggcaacgg catgacagac
     2461 agttgtattc gctggattag cgcgtccggc tgattaagct gcgaccagcc ccataatcct
     2521 gttccgccga gaccggccat cacaaccatt ccggccataa atatcggcca tgtccgctgc
     2581 cgggagattt caggggatgg cacgggtgga ctggcatcct tatcgttttg ctgaacggat
     2641 ctggttgcaa aaaccggctg tggactttcc tctgactttt tcggttgatt catttcccgg
     2701 cggggtaatt cagggggagt gataatggtt tccgctccct gaggcgccag ccgttccaga
     2761 cgcagagccg cattgcgaaa ctgcccggac aatacgtcca gcttcgtcat atgccacagt
     2821 tcacactgtt gcagcgtggt ttcgatggcg gaaagatgct gtacacaacg gtagatctgc
     2881 ggcagatcct gatactgaat atccagcgtt ctcagtgcct gctgcatttt ttctgtgacc
     2941 cagctgagca gtgatattct ggaatgtacc tgtaccggcc agaagtcagc ccagcgctgg
     3001 agcagtatat ccagtgaacc gagtccgtct gccatccctg ccagcccctg ccgtctggtt
     3061 attgccagca cgtaacagac cagcgtctgt agttccacgc cattttgccg gaacagcgcg
     3121 aggcagagtt tttccacctg acgccagtcc acatccggtc gggccgggtg agtcagttta
     3181 ctcatttcag cctgcagttc accggattcc ttataagtac agggatcgcc gcccatgacg
     3241 aactggctga taaaattcgc gttacttgcc atcagtacgc ccgctcctgt tgtttgagat
     3301 gacgcagaac aattcgtcct tccggagaaa atacgccatc aaagcgtatc cagccggtat
     3361 cgggaagctg cgcgtggatc aggtaattaa gatgccccgg accgggctgg ggcagtaact
     3421 ccacctgtat ctgattcagg cgtggttcat aacgcagcag cgtttcttca atctggcgca
     3481 tcaggctatg aatacccgca gccatcccct gatggatcag gctgagatcc ggcagaccgt
     3541 aatccggcaa atgcgccagt gtgcctgccc gacagttgat aatgcgttgc atattgtcca
     3601 tcacggaaag gataagctga tcccgctcac tgacctgttc cagcggtaac tcgccgctga
     3661 aactgaacgt cagcatttca tataaggaag gtgtcgccat cggttattta tccttgagag
     3721 gcagcagtcg caggtcgctt ctcatcagtt caatcgagcg cggcttgtcg gcctccagtt
     3781 cgtccctttt gatcaccaga cgccagctgt cgcttttttc atccgggtga taaaactgcc
     3841 caataatggc gacaaactgc gtctctttat ccagcggcac attcagggat gccgtgcctt
     3901 caggttttac ccagacggtg tgttttgcca gtacgtcctt ctccagcgtc ttctcttcct
     3961 gcataaacag ggtgtcataa tccgccttat caaaatcttc gcgggttttc agttgccaga
     4021 catgcaccat cgtggaaagc ggcgtatctt ccgcatccgt attcagggcg ctgcgggaga
     4081 agaaatcgag atgcagtatt ttgatttgtt tgtagaaaac ggctgaggcc acgcttttcg
     4141 attcatccac cactttctga atcagcccgc agccactcag aacaaccgtc agcatcgcca
     4201 tacagagcaa cgccggacgt ttaaaacgaa taggaaacat gagtcatact ctccctgtta
     4261 cgaatggaaa aagcgggtat taatccctga taacgcccca gactgaccgt cactgttccg
     4321 gtcgcgggcg cggcaaagga ggcgcgaagg atgccggtac ggctaatctg tacacgctgt
     4381 tttcccagcc gggcagcagg caacaggctg acgggcagcg tcagttgcag acgggcctga
     4441 cagcgccagc ccagataaac gcgcagcagc accagcaaat cgctgtgcag ttgcccgccg
     4501 ggcagccagc cacgtgcttc tgcggcatct tcggtataca gtttcagcag caactggctg
     4561 ttgacgtcgg taccggtacg ccccagcagc gttcgcgttt tcaggctgat gcgtgaattt
     4621 tttgacagac ccgcaggggc cgggagaatt acaggctgcg gatcgtgagg caccaccgtc
     4681 gctttggtca gcggggcaag cagccctacc agtgccgtga cgccttccgc cgtgcgcgtc
     4741 ggcaggcgca tcacgctgag cagggccaga aaacgggata ccggcgtggc gatatggttc
     4801 tcgctgccgg gaatacccag cccgatgagt cccagcagac aacgggaaat gtcatccacg
     4861 gcacctgctt caaacgacgc cgggtagtta tatttgcgcc agatgcggta gtactgagta
     4921 ataaagcgat ggttaaaaat atcgagaaac gccatcactg cgtcatgtcc gtcatgacgc
     4981 tgggtaatgt agtccagata ggcggttggc agcggcgaat ccacgccgta caggccgaga
     5041 aaagtggtgc gcaccgtggg gggcgcgtcg ggattgccca cgtcacgttc aacgtttttt
     5101 aactcgctga ccggaaagcc catacctgga tgaggacgaa agcgcaccgc atcggtggcg
     5161 gggctgtcgg tactgcccag cggcgcgttc tgctggctct gttccagcaa ctggcaaaaa
     5221 cggtaaaaat tgatccgcgc aatatccggg gccagccggt cggtcagccc ggtacgcgct
     5281 ggctgtgatg ctctgtccat tgcaggcact ttcctgtggg ttgcagaatt aaggtcagtt
     5341 gagtgaataa gtggatatcg gcatacagtg caaagaagcg gtgcagcagt tcgccaaaca
     5401 gcgtgatatc accctctccg gcaaagccgg tgctgtccag cgtcacctga atatccacgc
     5461 cacgcagcag aaagcctctt tcaaaacgct gtatcaggct gtgctgaacg tcgacaatcg
     5521 ccgacagacg gcggcggttc atctcgcttt cggtccagtc gtacagcgcc agcgtgccgc
     5581 gcagaatttc ggcgttatcc atcattgaaa gaaagttaga accgaggtgg ctgagcaccc
     5641 gccagtggaa gcggtcacgc gccgggggat aacagggctg cgtcggggcg cacaggttgc
     5701 gcacccgcag gacgttctgt gaggcatgaa ctggcgtatc aagcagcgtg ctctgtaaag
     5761 ccttacgggg aagctgtccg ttagtgccgg tcaggcttaa agagagcgtt tcatcctcca
     5821 gcatccggtc ggaatcaaac gcatcgccgc ccagaatcag ccaggtatca tgcaggccgg
     5881 aagggccgcg cttaacccgc gtatggtaat agcgttccgg tgcatcgtgg cgcagcatcc
     5941 cgccccggtg acgaaagctg gaaaacggga catacgcctg actgccggta tggcgcgaag
     6001 aaataatgtt gtcgacggaa taaatctcgg tatgcccgtc ctgaatacgc atcgggcgca
     6061 gcagaaattc gttttccagt ggcgaaaggt gtaacggatc ggcttccagc gggaacaggt
     6121 tgatgaccgg cacgcagtgc agacggatat tatcgctgtc aaacggcagg tcgtgaggcc
     6181 agttctcgct cagcaccacg tcaatctcaa agccgctcat cccttccggc cagatgacgt
     6241 tctccagccc gttcagcgcc acaaacataa acttttcgcg gaaggtgaaa tactccagca
     6301 gcaactggta gccgctgaac gccgactcgc cttttggcca cagtcggtca ttgtcatcaa
     6361 aacccatcgg ggaaaagtgc gcgtccagca cacggcgttc ctgccccggc agacgcagcc
     6421 agaactgctg ggtgccaagc gttaaagccc ggtgcagcgc acaggccacc gggctgtcgg
     6481 cattgagata gagcggcaaa cgggatagat cgatctgtga ccagtcacca accagcgggc
     6541 cgcattcaaa gcgcagacgg ataaccgagc gcccgtccgg ttcatattgc aatgacacgt
     6601 ccggcagcgc cagcggatgc agcgtgatat cccgcgtagc actgtagaca cagcgggttc
     6661 tgcgctcgcc aacaggacgc gacaatacct gaaaattact cagtgtttcg ctctgcttca
     6721 tctgccggtg atctgtcgac agttcaacca cggaaagtga cgggatggtt cgcaggtagt
     6781 gaggccacag caggctgacc agcccctcgg tcagttcggg caggtcgtca tccagctttt
     6841 cacgcaggcg acccattaaa aaagcgaagc cttcgaacag gcgctccaca tagggatcgc
     6901 gtgcgccggg tttatcaaga ttgagcatcg cggcgcggtc ggggtaagcc tgcgcaaatt
     6961 ctttccccgc ttcacgcagg tagcgcattt cggcttcgta gtagcgttgg gttagatcgt
     7021 ccatcggagg gattatcttc ctttcattga aagtgaatat catgcaggtg tagaagtaac
     7081 atcatagtta aatttattct atgtgaatat attatg
//
LOCUS       DAZGAP010000221         7067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGAP010000221 DAZGAP010000000
VERSION     DAZGAP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.222"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..3188
                     /locus_tag="ACHLAM_004555"
     CDS             <1..3188
                     /locus_tag="ACHLAM_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO7855987.1"
                     /translation="KPHVHIIIPEVNLLSGNLINPRGDYRVNEKYFEAVQEYINQKYN
                     LISPRERVRANPKNVSDILSRYKGDDFRSKNREFKEQLVRDIVEENILSRKDFYTHVS
                     SFGDIRIRNQGKENEYIAVKLPGDKKFTNLKETIFNDDFIVRRELKKPPLEKSVIQQL
                     LHEWPQRAMELKYIDKANSDFRKKYYSSSQEEKIKILAQQETAFYQKYGERYDVFPAE
                     WKTNHKSGINEAQTRGTTFSPNGLQSMSGGEMATGWQTGNTGSSLFLSTDALVFMGQS
                     QSSGRDSGLRCDVHSGGRGRSGNQRGSGQPAITGTNATVHSRRSTRTQYGNRYAGGLL
                     AGDVSIPVYARNKPGGATIQDVIRRSEILLRPPPGKSNISVNRFNGDIPQMGKNAAYV
                     AAHFVRQYEQNKILPNQRQALWDIRQLYQSTRSFIWQDERLTRKERSQYLSVLAFEHV
                     KARQKIINSSQQENTMASEDIRKLIKKKPRLSNNEITGKDERKIPPAGKRFSRIVHTL
                     KNRIEGEDKENSYAKSVSAADLYTRKSKLSKNVHYLDKNSNRTLFVDTGNAIAIRKDG
                     MSPAALGIALELAKERFGSTLTIKGNEEFRNQVIDIVARNHPDLHFSDKAMNRQLEER
                     RAEIAAERDGMTIEGVSKTSTSKEQEKEHEQAADTKNQTNVKESSEPHESPESRTVVK
                     LEGKLIDHGKAPLDNKKGAEQSYFVTVRGRDGKETTHWDSDLQNVMKGKRRGTEISLE
                     LKESNPVQVSTRDHLGNLSIREAVRQTWTLNKMSVGQEQKREQSLEKIQQNQSPIPAR
                     TQTAVDLILANPVLRQMAMTAEQKEREFTALQADKESVREQTDQEIRNRLGSGDQSRE
                     PLAVILSDDELRGMALSMEQKMREMATPVDKLDAVKAEINSEIAARVAWNSSIPVPVS
                     EVSATVAQKEIHREAESRGNKDKVHEGVLLDHGRAAYKFKPDISKPEDERDDSYFVKL
                     KMDNGKTRTLWGVGLESAVAGYSKGERLRLEDKGTEKVQWIERMKDGREIEKNGDRRV
                     WDCRSLTRESEVMKGFRVSGMPERDDGPDVA"
     gene            3592..3855
                     /gene="repA"
                     /locus_tag="ACHLAM_004556"
     CDS             3592..3855
                     /gene="repA"
                     /locus_tag="ACHLAM_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO7855988.1"
                     /translation="MSDAVRSATSSEARPKRAYRKGAPLSVNERKRLSLNRKRDTHKA
                     LNVFIRNELKDILERFCQDKHLTLGEGIELLIQQEKERAKNGE"
     gene            4078..4152
                     /gene="tap"
                     /locus_tag="ACHLAM_004557"
     CDS             4078..4152
                     /gene="tap"
                     /locus_tag="ACHLAM_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO7855989.1"
                     /translation="MPGKVQYFFLCSLLLRIVSAGWCD"
     gene            4145..5002
                     /gene="repA"
                     /locus_tag="ACHLAM_004558"
     CDS             4145..5002
                     /gene="repA"
                     /locus_tag="ACHLAM_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7855990.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALFSSLDI
                     SEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            6071..6334
                     /locus_tag="ACHLAM_004559"
     CDS             6071..6334
                     /locus_tag="ACHLAM_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFO7855991.1"
                     /translation="MNSTTHYENANFLRELAESLPRILPEGSTDKSALLQRLANEELA
                     RAEYDEQVRAKIAAARADKRPGISSAQLRQQLQGRYQELRNEL"
     gene            6324..6623
                     /locus_tag="ACHLAM_004560"
     CDS             6324..6623
                     /locus_tag="ACHLAM_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7855992.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLAENASLWDARNLTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDEASRVNILAVVGQRQNPRFII"
BASE COUNT         2097 a         1537 c         1768 g         1665 t
ORIGIN      
        1 gaaaaccaca tgttcatatt attattccag aggttaatct tctttctggt aatttaataa
       61 accccagggg agattacagg gtaaatgaaa aatattttga ggctgttcag gaatacataa
      121 accaaaaata taatctgatt tctccccgag agcgtgtcag agccaatcca aagaatgtgt
      181 ctgatatttt gtctcgttat aagggggatg atttcagaag taagaacaga gaatttaaag
      241 aacaactggt cagggatatt gttgaagaaa atattttatc aagaaaagat ttttatacac
      301 atgtatcttc atttggtgat atccgaattc gtaatcaggg taaagaaaat gaatatatcg
      361 ccgttaaatt accgggtgat aaaaagttta ccaacctgaa ggagacaata tttaatgatg
      421 actttattgt gagacgagaa ctgaagaaac ctccactgga aaaatctgtc attcagcagc
      481 ttttgcatga atggccacag cgagctatgg agcttaagta cattgataag gctaacagtg
      541 atttccgaaa aaaatattat tcatcctcgc aggaggagaa aataaaaatt cttgcacaac
      601 aagaaacagc attttatcag aagtacggag aaagatatga cgtattccct gccgaatgga
      661 aaacaaatca taagtccggt attaatgaag ctcagaccag aggaactacc ttctctccca
      721 atggcttgca aagtatgtcc ggtggcgaaa tggcaactgg ttggcaaact ggaaacaccg
      781 ggagttcgtt gtttctgtcc actgatgcac ttgttttcat gggacagtcg caatccagcg
      841 gaagagattc tggactgcga tgcgatgtac attcaggagg aagaggacga tctggtaatc
      901 aacgaggaag cggacaaccc gctattaccg ggacgaatgc caccgttcac tccagacggt
      961 caacaagaac tcagtacgga aatcgatatg ccggaggatt actggccggg gatgtgagta
     1021 ttcctgtgta tgcgcgtaat aaacccggag gggcaacaat acaggatgta attcgccgta
     1081 gtgaaattct tttacgacca cctccaggaa aatcaaatat cagtgtgaac aggtttaatg
     1141 gtgatatacc ccaaatgggg aaaaacgcag cttatgtagc cgctcatttt gttcgtcaat
     1201 atgaacaaaa taagatatta cctaatcaac gtcaggcatt gtgggacatt cggcagctct
     1261 atcagtcaac ccggagtttt atctggcagg atgaacgatt aactaggaaa gaacgctcac
     1321 agtatctgtc agtactcgct tttgagcatg taaaagcgcg tcagaaaatc attaatagta
     1381 gtcagcagga gaatactatg gcaagtgagg atatccgcaa acttattaag aaaaaaccac
     1441 gcctgtcaaa taacgaaatt acagggaaag acgagcggaa gataccaccg gcaggaaaac
     1501 gtttttcaag aatagttcat acactgaaaa accgaattga aggtgaagat aaagagaatt
     1561 catatgcaaa gtctgttagt gcagcggatt tatataccag aaaatcgaaa ctcagtaaga
     1621 atgtgcatta tcttgataaa aacagtaaca ggacattatt cgttgataca ggaaatgcca
     1681 ttgctataag aaaagatggt atgtctccgg cggcgctggg gattgctctt gaactggcca
     1741 aagaacgttt tggcagtaca ctgactatca aggggaatga agaatttcgt aaccaggtta
     1801 ttgatattgt cgccagaaat cacccagatc ttcatttttc tgacaaagcg atgaatcggc
     1861 aacttgaaga acgacgggca gaaattgccg ctgaacgtga tgggatgaca atagaaggcg
     1921 tttctaaaac gagtacgagt aaggaacaag agaaagagca tgaacaggca gcagatacta
     1981 aaaatcaaac aaatgtgaag gagtcctcag agccacacga atcacctgaa agcaggacag
     2041 tagttaagct ggagggtaaa ttaatagatc acggcaaagc gcctcttgat aacaagaagg
     2101 gtgctgaaca aagttatttt gttacggtca ggggcaggga cggtaaagaa accacacact
     2161 gggatagtga tttgcaaaac gtaatgaagg ggaaacgccg gggaacagag atatctcttg
     2221 agttgaaaga atctaatccg gttcaggtca gtacacgcga tcatctcggc aatttgtcta
     2281 tcagggaagc ggtccggcag acttggacat taaacaagat gtcggtggga caggagcaga
     2341 aaagggaaca gtcgctggag aaaatacagc aaaatcaaag ccccatacca gccagaacgc
     2401 agacagctgt tgaccttatt cttgctaatc cagtattacg gcagatggct atgactgcgg
     2461 aacaaaagga aagagaattt actgcattgc aggcagataa agaatccgtc agagaacaga
     2521 ctgatcagga aataagaaat cgtctcggta gtggagatca aagcagggaa ccgttagccg
     2581 tcattttgtc agatgatgaa ctacgtggaa tggcgttatc gatggagcaa aaaatgcggg
     2641 aaatggctac tccagtagat aaattggatg cagttaaagc agaaattaac agtgaaattg
     2701 cagcgagggt tgcgtggaac agcagtattc ctgtgcctgt atcagaagtt agcgccactg
     2761 tggcacaaaa agaaatccat cgggaagcag agtcgagagg taataaagat aaagtacatg
     2821 aaggtgtgct acttgatcat ggccgggcag catacaaatt taagcctgat ataagcaaac
     2881 cagaagatga acgtgatgat agctattttg tgaaactgaa gatggataac gggaaaacac
     2941 gaacactatg gggagttggt ctggagagtg ctgtagcagg ttatagtaaa ggtgagcgtt
     3001 tgcgactgga ggataaagga acagagaaag ttcagtggat tgaaaggatg aaggatggaa
     3061 gagagattga aaaaaatggc gatcgcaggg tttgggactg tagatcgctg actcgtgaat
     3121 ctgaagtcat gaagggattc agagtatcag gtatgccgga gcgtgatgat ggtcctgatg
     3181 tagcgtaact tatacaccgc gccttatatc cccgccctta agggcggggt tttaaaacca
     3241 gcgggtcgag caaggcaagt tccgaccgtt aacgtaatcc cacattcaag aacttacggt
     3301 ttcattcttt gttcagcagt tgaaaccgta agtttctgtt caaatgccgt ttcgtgagct
     3361 gctccctttt tgatccggga tttgtggagg gtgttagtta agtaatcaca tgatatatcg
     3421 acacttgttc cgttcggagc aggatctttt ctgaatagca tcagatcttt atagctgaac
     3481 tggcgatctg tgcgccaaca tggatccgcc agggtataat taagtcgctg atttcaataa
     3541 agtgtagtat cctctgcaaa atgatccttt tttgatctat gaggaggctg tatgtctgac
     3601 gctgtacgtt cggcaacttc ctctgaagcc agacctaaac gggcatatag aaaaggtgca
     3661 ccgttaagcg ttaatgagcg aaagaggttg tctctcaatc gaaagcggga tacacataaa
     3721 gcgcttaatg ttttcatccg taatgaattg aaagatattc ttgagcgctt ttgccaggat
     3781 aagcatttga ccttgggtga agggatcgag ctgctaatcc agcaggagaa ggaacgagct
     3841 aaaaatggtg agtagtcacc tttatttgat ccttttctgg cagagtgata gattacggac
     3901 agaaaattca tggctggcca cgccgtaagg tggcagggaa ctggttctga tgtggattta
     3961 caggagccag aaaagcaaaa accccgataa cctttctatg tttggcgacg cagaagatta
     4021 ccgggggcct acaaaaccgt atagccaaca attcagctat gcggggagta tagttatatg
     4081 cccggaaaag ttcaatactt ctttctgtgc tcactccttc tgcgcattgt aagtgcagga
     4141 tggtgtgact gatcttcaac aaacgtatta ccgccaggta aagaacccga atccggtgtt
     4201 cacaccccgt gaaggtgcag gaacgctgaa gttctgcgaa aaactgatgg aaaaggcggt
     4261 gggcttcacc tcccgttttg atttcgccat tcatgtggcg catgcccgtt cgcgtggtct
     4321 gcgtcgacgc atgccaccgg tgctgcgtcg acgggctatt gatgcgctcc tgcaggggct
     4381 gtgtttccac tatgacccgc tggccaaccg cgtgcagtgc tccatcacca cgctggccat
     4441 tgagtgcgga ctggcgacgg agtctgaggc cgggaaactc tccatcaccc gtgccacccg
     4501 tgccctgaag ttcctgtctg agctggggct gataacctac cagacggaat acgacccgac
     4561 aattggctgt aacatcccga ccgacatcac gttcacgccg gcactgtttt cctcactcga
     4621 tatatcagaa gaggcggtgg cttccgcacg ccgcagccgc gtggaatggg aaaacaagct
     4681 gcgcaaaaag caggggctgg atgcgctggg tatggatgaa ctgatagcga gagcctggcg
     4741 ttttgtgcgc gagcgtttcc gtagttatca ggcagagctt aagtcccacg gcatgaaacg
     4801 tgcccgtgcg cgccgcgatg caggcaggac ccgtcaggac attgtcaccc tggtgaaacg
     4861 gcagctgaca cgtgaaatcg cggaagggcg tttccgcgga agcctggagg ccgtgaagcg
     4921 cgaaatcgac cgtcgcgtga aggaacgcat gataatgtcg cggaacaaca actacacacg
     4981 gctggccaca gcctccccct gagttttacc tcatctgaat aatccggcct gcgccggagg
     5041 cttccgcacg tctgaagccc ggcgacgcac aaaaaagcag ctcagcacat aaaaaacagc
     5101 ctcatcatct gctttccggc tcgcaatgct cccccggttt ttcacgcaaa acacgtctca
     5161 cagacggaga attccgctta tccacattta actgcaaggg aattctccat aaggttacaa
     5221 tcgttcatgt cataaagcgc cagccgcagg cgttacaggc tgcaatgttt cttttaaacc
     5281 ccagtactgt atttccttta aactacttaa ttacttccat ttaaaaagaa aacctattca
     5341 ctgcctgtcc tgtagacaga cagatatgca cctcccaccg caagcggcgg gccccgaccg
     5401 gagccacttt agttacaaca ctcaaataca agcaccggaa aaaacccggt cccgcgcaga
     5461 actgaaacca caaagccctt ccctcataac tgaaaagcgc cgccgccccc gcccgaaggg
     5521 cgggaacaac atcgctttta atcatgaatg ttgtaactac aatccatcat cgctgtcagt
     5581 cttctggctg gatgttctca gtacacgctc gtaagcggcc ctgacggccc gctaacgcgg
     5641 agatacgccc cgacttcggg taaaccctcg tcgggaccac tccgaccgcg cacataagct
     5701 ctgtcatggc tgacagcggg tatagctttg cagggtgggg atgggtaagg tgaaatctat
     5761 taatcagtac cggcttacgc cgggcttcgg cggttttact ccggtatcat atgaaacaac
     5821 agagtgccgc cttccatgcc gctgatgcgg cataagcata tgttggtggc tgtgtctgaa
     5881 aggttatata ctctgcataa aacctgacaa cacgcagatt gccaggcaaa tgagcattat
     5941 ttttccggtt attgcctgcc tgtgaaacaa tatcaccggg gcgaatatcg atactctgac
     6001 tcgttatact catgcgtgcg gtatcaaaaa atctgcaatt accctttttg aatgaagggg
     6061 ggagtcaact atgaatagca ccacacatta cgaaaacgcc aatttcctgc gcgagctggc
     6121 cgaaagcctg cctcgtattt tgccagaagg cagtacagac aagtctgcac tattgcaacg
     6181 cctcgctaat gaagaactgg cacgggcgga atacgacgaa caggtacgag cgaaaattgc
     6241 cgctgctcgc gccgataagc gaccgggaat aagttccgcg caactgcgcc agcaactaca
     6301 gggacgttat caggaactgc gcaatgagtt atgaaataga atggcgacca ggtgcgattg
     6361 aggatataac ggcgttgttt gaatatctgg ccgagaatgc atctctttgg gatgcaagga
     6421 acttgacaga gcgtctgatc actgcaaccg atcgcctggc agattacccc aggctgtatg
     6481 aaacggatga acgctatggt gaaggggttc gccgtattag cttacttgga cagcatgtac
     6541 tttatgaagt gaatgatgag gccagtcgtg tcaatatctt ggcggttgtc ggccagcgtc
     6601 agaatccccg attcattatt tgagcgcccg gcttggtgct cagcttaccg gacttgccag
     6661 gaattacggt ttgggagccg cttggagaaa ggaaattttc tcacgctaag agaattgagt
     6721 tgaacgagtg gttacatcca ggccagctaa ctcactgaac gtaaaggctg tacgaaatgt
     6781 tccgaccgtc cgataaccat ttggcttact tccgtacatt tccttgcgta gatgtacaga
     6841 attgtacgat ttcggacacc aaaaccgctg ttgtcccgcc attctggcga tttctgaaag
     6901 ttatttgact aaaaaatagt cttaacgtgg tttattttcc gaattccaag cggaccccta
     6961 agagaaaagc aataacttaa aggcgatagc tgatcacgat gaatcaatca gttacttatc
     7021 cgcatcgatt aaaaaatgct cctaacgtac aataatttcc gtttccc
//
LOCUS       DAZGAP010000222         7032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGAP010000222 DAZGAP010000000
VERSION     DAZGAP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.223"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            582..1571
                     /gene="araF"
                     /locus_tag="ACHLAM_004561"
     CDS             582..1571
                     /gene="araF"
                     /locus_tag="ACHLAM_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO7855993.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1641..3155
                     /gene="araG"
                     /locus_tag="ACHLAM_004562"
     CDS             1641..3155
                     /gene="araG"
                     /locus_tag="ACHLAM_004562"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO7855994.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGWVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3170..4156
                     /gene="araH"
                     /locus_tag="ACHLAM_004563"
     CDS             3170..4156
                     /gene="araH"
                     /locus_tag="ACHLAM_004563"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO7855995.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4323..5123
                     /gene="otsB"
                     /locus_tag="ACHLAM_004564"
     CDS             4323..5123
                     /gene="otsB"
                     /locus_tag="ACHLAM_004564"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO7855996.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5098..6522
                     /gene="otsA"
                     /locus_tag="ACHLAM_004565"
     CDS             5098..6522
                     /gene="otsA"
                     /locus_tag="ACHLAM_004565"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO7855997.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6529..6957)
                     /gene="uspC"
                     /locus_tag="ACHLAM_004566"
     CDS             complement(6529..6957)
                     /gene="uspC"
                     /locus_tag="ACHLAM_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO7855998.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1760 a         1648 c         1830 g         1794 t
ORIGIN      
        1 taactgcaat gaattttaat gatagtgctt tttgtatctt gttgatatta atcgattaat
       61 ttgaattcat gatgaagtga tggttttata aaacgttttt tcatttttgc gagatggctc
      121 tcattatacg tgttctgtta attaatgagc acagtgataa ttaatggcga aatgaatgac
      181 gcacattttc cacatctttg agttgtggtt attacaccat tgcaaaaact caacgccagg
      241 taatgcggcc tattgactgg ttaaaaagaa gacatcccgc atgggtacca aagacaacaa
      301 ggacttccag gctaatctta tggattattc tgctgtgcac tcgataattt gtctgacaaa
      361 ttcgcttttc cttatgtctt ttcccgctaa atttatgcac gttctcactg taattctgcg
      421 atgtgatatt gctctcctat ggagaattaa tttctcgcta aaactatgtc aacacagtca
      481 cttatctttt agttaaaagg taatgctttg ttttccgatt aatttaacga atgtcattcg
      541 tttttgccct acacaaaacg acactaaagc tggagagaac catgcacaaa tttactaaag
      601 ccctggcagc cattggtctg gcagccgtta tgtcacaatc cgctatggcg gagaacctga
      661 agctcggttt cctggtgaag caaccggaag agccgtggtt ccagaccgaa tggaagtttg
      721 ccgataaagc cgggaaagat ttagggtttg aagttattaa gattgccgtg ccggatggcg
      781 aaaaaacatt gaacgctatc gacagcctgg ctgccagtgg cgcgaaaggt ttcgttattt
      841 gtactccgga cccgaaactc ggctctgcca tcgtggcgaa agcgcgtggc tacgatatga
      901 aagtcattgc cgtggatgac cagtttgtta acgccaaagg taagccaatg gataccgttc
      961 cgctggtgat gatggctgcg accaaaattg gcgaacgtca gggccaggaa ctgtataaag
     1021 agatgcagaa acgtggctgg gatgtcaaag aaagcgcggt gatggcgatt accgccaacg
     1081 aactggatac cgcccgccgc cgtacaacgg gttctatgga cgcactgaaa gctgcaggat
     1141 tcccggaaaa acaaatttat caggtaccta ccaaatctaa cgacatcccg ggggcatttg
     1201 acgctgccaa ctcaatgctg gttcaacatc cggaagttaa acattggctg atcgtcggta
     1261 tgaacgacag caccgtgctg ggcggcgtac gcgcgacgga aggtcagggc tttaaagcgg
     1321 ccgatatcat cggtatcggt attaacggtg tggatgcggt gagcgaactg tctaaggcac
     1381 aggcaaccgg cttctacggt tccctgctgc caagcccgga cgtacatggt tataagtcca
     1441 gcgaaatgct ttacaactgg gtagcaaaag acgttgaacc gccaaaattt accgaagtta
     1501 ccgacgtggt gctgatcacg cgtgacaact ttaaagaaga actggagaaa aaaggtttag
     1561 gcggtaagta atttgccgga aagattcccc tctgcatgat gcagaggggg tgtgaacgac
     1621 cagtgattca cggagacgtt atgcaacagt ctaccccgta tctctcattt cgcggcatcg
     1681 gtaaaacgtt tcccggcgtt aaggcgctga cggatattag ttttgactgc tatgccggtc
     1741 aggttcatgc gttgatgggt gaaaatggcg caggaaaatc aactctctta aaaatcctca
     1801 gcggaaacta tgcgccaacc acgggttctg tagtgattaa tgggcaggaa atgtcctttt
     1861 ccgacacgac cgcagcactt aacgcggggg tggcgattat ttaccaggag ctgcatctcg
     1921 tgccggaaat gaccgtcgcg gaaaacatct atctcggcca gctgccgcat aaaggcggca
     1981 ttgtcaatcg ctcattgctg aattatgagg cgggtttaca acttaaacat cttggtatgg
     2041 atattgaccc ggacacgccg ctgaaatatc tctccattgg tcagtggcag atggttgaaa
     2101 ttgccaaagc cctggcgcgt aatgccaaaa ttatcgcctt tgatgagcca accagctccc
     2161 tctctgcccg tgaaatcgac aatcttttcc gcgttattcg tgaactgcga aaagaggggt
     2221 gggtgatctt atacgtttct caccgtatgg aagaaatatt tgccctcagc gatgccatca
     2281 ctgtctttaa agatggacgt tatgtcaaaa cctttaccga tatgcagcag gttgaccacg
     2341 acgccctggt gcaggcgatg gtcgggcgcg acatcggcga tatctacggt tggcaaccgc
     2401 gtagttatgg cgaggagcgg ctgcgtcttg atgctgtgaa agcaccaggc gtgcgtacgc
     2461 caataagtct ggcggttcgc agtggagaaa ttgtcggtct gtttggtctg gtcggggcag
     2521 ggcgtagcga attaatgaaa ggcatgtttg gcggtacgca aatcaccgcc ggtcaggttt
     2581 atatcgacca acagccgatc gatattcgta aaccgagcca cgccattgcc gcaggcatga
     2641 tgctctgccc ggaagatcgc aaagcggaag gcattattcc cgtgcactcc gttcgcgaca
     2701 atatcaacat cagtgccaga cgtacacatg tgctcggcgg ttgtgtaatc aacaacggtt
     2761 gggaagaaaa caatgccgat caccacattc gttcgctcaa catcaaaacg ccgggcgctg
     2821 atcaactgat catgaatctc tcaggcggaa atcagcaaaa agccattctg ggccgctggt
     2881 tatcggaaga gatgaaggtc attttgctgg atgaacctac gcgcggcatt gatgttggtg
     2941 ctaagcacga aatatataac gtaatttatg cgctggcggc gcagggtgtg gcggtgctgt
     3001 ttgcctccag cgacttacct gaagtcctcg gcgttgccga ccggattgtg gtgatgcggg
     3061 aaggcgaaat cgccggtgaa ttgttacacg agcaggcaga tgagcgtcag gcactgagcc
     3121 ttgcgatgcc taaagtcagc caggcagttg cctgagtaag gagagtatga tgtcttctgt
     3181 ttctacatcg ggttctggcg cacctaagtc gtcattcagc ttagggcgta tctgggatca
     3241 gtacggcatg ctggtggtgt ttgcggtcct ttttatcgcc tgtgccattt ttgtcccgaa
     3301 ctttgccacc tttattaata tgaaagggtt gggtctggca atttccatgt cggggatggt
     3361 ggcttgtggc atgttgttct gccttgcttc cggtgacttt gacctttctg tcgcctccgt
     3421 aattgcctgt gcgggtgtca ccacggcggt ggttattaac ctgactgaaa gcctgtggat
     3481 tggcgtggca gcgggtttgt tgctgggcgt tctctgtggc ctggtcaatg gctttgttat
     3541 tgccaaactg aaaataaatg ccctgatcac gacactggca acgatgcaga ttgttcgagg
     3601 tctggcgtac atcatttcag acggtaaagc ggtcggtatc gaagatgaaa gcttctttgc
     3661 ccttggttac gccaactggt tcggtctgcc tgcgccaatc tggctcaccg tcgcgtgtct
     3721 gatcatcttt ggcttgctgc tgaataaaac cacctttggg cgtaataccc tggcgattgg
     3781 cgggaacgaa gaggccgcgc gtctggcggg tgtaccggtt gttcgcacca aaattattat
     3841 ctttgttctc tctggcctgg tatcagcgat agccggaatt attctggctt cacgtatgac
     3901 cagtgggcag ccaatgacgt cgattggcta tgagctgatt gtgatctccg cctgcgtttt
     3961 aggtggcgtt tctctgaaag gcggcatcgg aaaaatctca tatgtggtgg cgggtatctt
     4021 aattttaggc accgtggaaa acgccatgaa cctgcttaat atttctcctt tcgcacagta
     4081 cgtagttcgc ggcttaatcc tgctggcagc ggtgattttc gaccgttaca agcaaaaagc
     4141 gaaacgcact gtctgatgct tttttccaca acaatttagc gttttttccc accacagcca
     4201 gccgccacaa cggttggctg ttcttcgttg caaatggcga cccccgtcac actgtctata
     4261 cttacatgtc tgtaaagcgc gttctgcgca acacaataag aaaagagaag gaggagaacc
     4321 gggtgacaga accgttaacc gaaacccctg aactatccgc gaaatatgcc tggttttttg
     4381 atctcgatgg aacgctggcg gaaatcaaac cgcatcccga tcaggtcgtc gtgcctgaca
     4441 ctattctgca aggacttcag ctactggcaa ccgcaagtga tggtgcattg gcattgattt
     4501 cagggcgctc aatggtggag cttgacgcac tggcaaaacc ttatcgcttc ccgttagcgg
     4561 gcgtgcatgg ggcggagcgc cgtgacatca atggtaaaac gcatatcgtt catctgccgg
     4621 atgcgattgc gcgtgatatt agcgtgcaac tgcatacggc tatcgcccag tttcccggtg
     4681 cggagctgga gacgaaaggg atggcctttg cgctgcatta tcgtcaggct ccgcagcatg
     4741 aagacgcatt aatgacatta gcgcaacgta ttactcagat ctggccacaa atggcgttac
     4801 agcagggaaa gtgtgttgtc gagatcaaac cgcgaggcac cagtaaaggt gaggcgattg
     4861 ctgcctttat gcaggaagct ccctttatcg ggcgaacgcc tgtatttctg ggcgatgatt
     4921 taaccgatga atctggcttc gcagtcgtta accggctggg cggaatgtca gtaaaaattg
     4981 gcacaggcgc aactcaggca tcatggcgac tggcgggcgt gccggatgtc tggagctggc
     5041 ttgaaatgat aaccaccgca ttacaacaaa aaagagaaaa taacaggagt gatgactatg
     5101 agtcgtttag tcgtagtatc taaccggatt gcaccaccag acgagcacgc cgccagtgcc
     5161 ggtggccttg ccgttggcat actgggggca ctgaaagccg caggtggact gtggtttggc
     5221 tggagcggtg aaacagggaa tgaggatcag ccgctaaaaa aggtgaaaaa gggtaacatt
     5281 acgtgggcct cttttaacct cagcgaacag gaccttgacg aatactacaa caaattctcc
     5341 aatgccgttc tctggcccgc ttttcattat cggctcgatc tggtgcaatt tcagcgtcct
     5401 gcctgggatg gctatctacg cgtaaatgcg ttgctggcag ataaattact gccgctgttg
     5461 caagacgatg acattatctg gatccacgat tatcacctgt tgccctttgc gcatgaatta
     5521 cgcaaacggg gagtgaataa tcgcattggt ttctttctgc atattccttt cccgacgccg
     5581 gaaatcttca acgcgctgcc gacatatgac accttgcttg aacagctttg tgattatgat
     5641 ttgctgggtt tccagacgga gaacgatcgt ctggcgttcc tggattgcct ttctaacctg
     5701 acccgcgtca cgacacgtag cgcaaaaagc catacggcct gcggcaaagc gtttcgaacg
     5761 gaagtttacc cgatcggcat tgaaccgaaa gaaatagcga aacaggctgc cggaccactg
     5821 ccgccaaaac tggcgcaact taaagcggaa ctgaaaaacg tacaaaatat cttttctgtc
     5881 gaacggctgg attattccaa aggtttgccg gagcgttttc tcgcctatga agcgttgctg
     5941 gaaaaatatc cgcagcatca tggtaaaatt cgttataccc agattgcacc aacgtcgcgt
     6001 ggtgatgtgc aagcctatca ggatattcgt catcagctcg aaaatgaagc tggacgaatt
     6061 aatggtaaat acgggcaatt aggctggacg ccgctttatt atttgaatca gcattttgac
     6121 cgtaaattac tgatgaaaat attccgctac tctgacgtgg gcttagtgac gccactgcgt
     6181 gacgggatga acctggtggc aaaagagtat gttgccgctc aggacccagc caacccgggc
     6241 gtacttgttc tttcgcaatt tgcgggagca gcaaacgagt taacgtcggc gttaattgtt
     6301 aatccctacg atcgtgacga agttgcagct gcgctggatc gtgcattgac tatgtcgctg
     6361 gcggaacgta tttcccgtca tgcagaaatg ctggacgtta tcgtgaaaaa cgatattaac
     6421 cactggcagg agtgcttcat tagcgaccta aagcagatag ttccgcgaag cgcggaaagc
     6481 cagcagcgcg ataaagttgc tacctttcct aagcttgcgt aggagcggtt aatctcccgt
     6541 aagtggaact aacagcacat cgacctcact tgaggcaatc actcttttcg ccgagcagga
     6601 tgctcgcgaa aagaaactgt gattgtgatt accgcaaatc accaaatcga aatgatattt
     6661 gcgacaaact tccagaatat gttcgcttaa ctcaccgtag gcaataaacg tcttgtcaac
     6721 gggatacccc gcatcctgaa ttaacttatc aagaaagctt tgcgtttctt cctgcatcac
     6781 gctgcgtaaa tcttcaagca tcggcgcagc taattgattg tacatttccg ggtcggaagc
     6841 gagagtaatt aaactgatgt gtccctttac tggcctggcg atagataccg cttttgccag
     6901 cagttgctga ctttccggtg taaccgcaac agcgacaaga atattgctat agctcataac
     6961 ctgttcctta ttctgtgaac ttcaggtgac attaaagctt tactcctaat ggagaaacga
     7021 cgcaatccca ac
//
LOCUS       DAZGAP010000223         7004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGAP010000223 DAZGAP010000000
VERSION     DAZGAP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.224"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..575
                     /gene="ftnA"
                     /locus_tag="ACHLAM_004567"
     CDS             78..575
                     /gene="ftnA"
                     /locus_tag="ACHLAM_004567"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO7855999.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(613..852)
                     /locus_tag="ACHLAM_004568"
     CDS             complement(613..852)
                     /locus_tag="ACHLAM_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO7856000.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1042..2253
                     /gene="tyrP"
                     /locus_tag="ACHLAM_004569"
     CDS             1042..2253
                     /gene="tyrP"
                     /locus_tag="ACHLAM_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO7856001.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2304..2969)
                     /gene="yecA"
                     /locus_tag="ACHLAM_004570"
     CDS             complement(2304..2969)
                     /gene="yecA"
                     /locus_tag="ACHLAM_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO7856002.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIESAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3165..3251)
                     /locus_tag="ACHLAM_004571"
     tRNA            complement(3165..3251)
                     /locus_tag="ACHLAM_004571"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3215..3217),aa:Leu,seq:taa)
     gene            complement(3264..3337)
                     /locus_tag="ACHLAM_004572"
     tRNA            complement(3264..3337)
                     /locus_tag="ACHLAM_004572"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3303..3305),aa:Cys,seq:gca)
     gene            complement(3392..3467)
                     /locus_tag="ACHLAM_004573"
     tRNA            complement(3392..3467)
                     /locus_tag="ACHLAM_004573"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3432..3434),aa:Gly,seq:gcc)
     gene            complement(3619..4167)
                     /gene="pgsA"
                     /locus_tag="ACHLAM_004574"
     CDS             complement(3619..4167)
                     /gene="pgsA"
                     /locus_tag="ACHLAM_004574"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO7856003.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4224..6056)
                     /gene="uvrC"
                     /locus_tag="ACHLAM_004575"
     CDS             complement(4224..6056)
                     /gene="uvrC"
                     /locus_tag="ACHLAM_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO7856004.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPTLHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6053..6709)
                     /gene="uvrY"
                     /locus_tag="ACHLAM_004576"
     CDS             complement(6053..6709)
                     /gene="uvrY"
                     /locus_tag="ACHLAM_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO7856005.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1708 a         1812 c         1650 g         1834 t
ORIGIN      
        1 tttacctttt gcaaataata aaataacaaa ttatagtgac gccacagcaa caaatataac
       61 ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat gagcagatga
      121 acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc agctatcata
      181 ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca ggaagagatg acgcatatgc
      241 agcgtctgtt tgattacctg actgataccg gcaacttacc gcgtattaat accgttgaat
      301 ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat aaacacgaac
      361 aattaatcac gcagaaaatt aacgaactgg ctcatgctgc aatgaccaat caggactacc
      421 caacatttaa tttcctgcaa tggtatgttt ctgagcagca tgaagaagag aaactgttca
      481 aatcgattat tgataaatta agcctggcag gcaaaagtgg cgaaggtctg tattttatcg
      541 acaaagaact ctctaccctc gacacacaaa actaatgctc tccggcggca ggttttctgc
      601 cgccgtttca aatcagtgac ggcaaatctt actctgatcg gttgaaaaac cttcttctga
      661 aggtataaat ttgccttttg ctgccagaaa cgccaccaac tctcccgcag tcatcccttc
      721 tgccgagcag gtgtgaaaac gtgcctgttc accaaaacgc gctttaattg ctgcttccag
      781 actggcatgc gtatattgct cgcctgattc aatcatcata tttaacactt cgtgaccgtg
      841 aatagagtcc atcgttcctc ctcaaaaaaa gcctagcgta gcgattgccg cttatgaaga
      901 ctttgcgcca gcgcagaact gaacgctttt attgtacatt tatatttaca tcatatgtaa
      961 cgccggtttg acgaatcagc cgttatgcct taacctgcgc cgcagatatc actcataaag
     1021 atcgtcagga cagaagaaag cgtgaaaaac agaactctgg gaagtgtttt tatcgtggcg
     1081 ggaaccacaa ttggcgcagg catgctggca atgccgctgg ctgcggcagg tgttggtttt
     1141 agcgtcacgt taatcttgtt aattgggctt tgggcgttga tgtgctacac ggcgctatta
     1201 ctgctggagg tgtaccagca tgtcccggca gataccggtc tgggcacgct ggcaaaacgc
     1261 tatctgggac gctacggtca gtggctgacg ggcttcagta tgatgttctt aatgtatgct
     1321 ctgactgcgg catacatcag cggtgccggt gaattgttgg cctccagcat cagcgactgg
     1381 acaggtattt caatgtcggc aaccgctggc gtgctgttgt tcacttttgt tgccggtggc
     1441 gtggtttgtg tcggaacttc gctggtcgat ttatttaacc gttttctgtt cagcgccaaa
     1501 attatttttc tggtggtgat gctggtattg ctgctaccgc atattcacaa agtgaatctt
     1561 ttaaccctgc cgttgcaaca gggactggca ctgtctgcaa tcccggtgat ttttacgtcg
     1621 tttggttttc acggtagcgt gccgagtatt gtcagctata tggatggcaa cattcgtaag
     1681 ctacgctggg tgtttataat cggtagtgcg atccccctgg tggcatatat tttctggcag
     1741 ctggcgaccc ttggcagcat tgattcaaca acctttatgg gattgctggc taatcatgcc
     1801 ggattaaacg ggctgttaca ggcgttacgc gaaatggtgg cctctccgca tgttgagctg
     1861 gcagtgcatt tatttgctga tttagccctc gccacgtcct tcctcggcgt agcgttaggc
     1921 ttatttgatt atctggcaga tttgtttcag cgttcaaata ccgttggtgg acgattgcaa
     1981 actggtgcaa ttacctttct gccgccactg gcgtttgcac tgttttatcc acgaggattt
     2041 gtgatggcgc tgggttacgc cggtgtggcg ctggcggtac tggcattgat tatcccttcg
     2101 ctgttgacct ggcaaagcag gaagcacaat cctcaggcgg gttaccgggt gaaaggtggt
     2161 cgtccggcgc tggtggtggt gtttctctgt ggtattgctg tgattggcgt gcaatttttg
     2221 attgcggcag gcttgttacc agaagtgggg tgattagatt cttattgggt gccagaatta
     2281 acgctgacac ccaatttgac ctcttaatgc aggcagcact gcttaaattt cttaccacta
     2341 ccgcacggac aaggatcgtt acgccccggt ttctcttctg ctttgatagg ttgctgaaca
     2401 gctttttcct gcggatgcgc catccagtat gcatgtaaat caagcgccgc cagtcgaata
     2461 gcatctacgc tctcttcaaa cgcttctggc gacatcttct ctacccgctc gaagttttcc
     2521 tcagtaccgt gcagcgcaat cgcatccagc gctggtttta acgaatcggg caacgttgac
     2581 cagtcagaaa gtgccacgcc ccgcatatag ccaaagcacc actcctcaac aatcgtcagc
     2641 tcgctgccat cgacttctcg caagccgaat aacggctcaa actgctccgg gaattcgttc
     2701 agacgctctg cggtatcagc catatgttga aaagccaggt tcataaagcg cgtcatctct
     2761 ttctctgacg cccagcgcgg cacatagtca gccccacccc acacggcaac cagccactgt
     2821 gccgattcaa tctcttgcgg agaactcaac accgccgtca ataaaccgtc cagctccgcc
     2881 acatcaagaa tggcgtggtc agtgttgtat ttggtcagaa tatcgtccag ccattccaac
     2941 tcactttcgt ttaacggtcc cgttttcata cgcttttcct tgtggatctc aactcgccag
     3001 cacctatctt acatgccggt ccgtatcaga gatacttttt gagtggcttt gccggtgatt
     3061 aaaaattaag gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt
     3121 tcacgacact gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg
     3181 acttgaaccc gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc
     3241 accatccggg ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga
     3301 tttgcaatcc gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca
     3361 gacatccgct tgacgccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg
     3421 accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcatt catcaagcaa
     3481 tcagttaatc acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc
     3541 attctactta cctggcgcga tgagtcaacg atatttttca ccacttttga tcgtttgctg
     3601 aaaattgcgc cgaaacgatc actgatcaag caaatctgca cgcgcagcgc tcaaatattg
     3661 caacattgac cacagagtca gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc
     3721 aacccaaatg ttcggacgcc acagcagcca ggccagcgcc accatctggg cagtggtttt
     3781 cactttccca atccaggaga cagccacgct actgcgttta cccaactccg ccatccattc
     3841 gcgtaacgca gaaataataa tttcacgggc gatcatcgtt gctgccggta aggtcaccca
     3901 ccagctgtga taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc
     3961 tacagggtca aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc
     4021 atcgaaccag tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca
     4081 ggtgacaggc agataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt
     4141 aagcaacgta gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt
     4201 acccctatgt tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga
     4261 ccttgcgaaa tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc
     4321 aaaccgccca tatatttcaa caacatttgc cgacgttttg gcccgacgcc ttcaatggtt
     4381 tccagggaac tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga
     4441 tcgtgtgatt catcgcgaat atgctggata acatgcagtg tgggagaatc tggcggcaaa
     4501 ctgaatccct caccttccgg ctcaaagaac agcgtttcca gcccagcctt acgatctgct
     4561 cctttggcaa cgcctagtag cagcggatga tttttatccc atgagacatc cagttcggcg
     4621 aaaacatttt tcgcctgcgc aagttggcct ttgccgccgt cgataaggat cacatccggg
     4681 atcttactct cgtcgatggc tttaccataa cgccgacgca gcacctgatt catcgccgca
     4741 taatcatcgc ccggcgtgat gccagtaatg ttatagcgtc gatactccgc acgcagcggg
     4801 ccgttagcat caaacaccac acaggaagcg acggtttgtt cccccatggt atggctgatg
     4861 tcaaagcatt ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt
     4921 cgctggtgaa cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg
     4981 cgcgcgagtt tcagataacg cgcccgatcg ccgcgaggtt tggtttgaac attaatcttg
     5041 cgtcccgcca gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg
     5101 agcaggatct cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctgacctacg
     5161 aaggtttcca ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg
     5221 ctgccgagca ctttaccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg
     5281 aacgccacac caataacgtc gaggtcgtcg ccggtattgg acacaaactg tttttcggtg
     5341 acgcgtcgca ccgcctgaat ttggtcgcga atacgtgccg cttcttcaaa ctccagattc
     5401 tggctggcag tttccatacg actaatgagt tgcgtaagca cctgatcatc tttgccagac
     5461 aaaaacaggc gcacatactc gacctgctga gcgtattctt cttcactcac cagtccttca
     5521 acgcacggtc ccagacagcg ccctatctga tattgcagac acggacgcga gcgattgcga
     5581 taaacactat tttcgcactg gcgaatgggg aatatctttt gcagtagcgc cagtgtttca
     5641 cgtacggcat agccattcgg gaacgggccg aaatattcac ctttagcatg cttcgcacca
     5701 cgatgcatcg ccagacgcgg atgggtatcg ccactcagga agataaaggg ataagattta
     5761 tcatcgcgta gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc
     5821 aacagcgctt cggtttctgt atgagtaacc gttacatcaa tttgctggat ctgggcgacc
     5881 agcgcttcgg ttttgcgcga agcgaggttg ctacggaaat agctggaaag ccgttttttc
     5941 aggtctttcg ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg
     6001 cctggctggc tggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac
     6061 ttgataatgt ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat
     6121 cgccatgaat gtttagttta ctgaacatac gatagcggta gctgttcacc gttttcggac
     6181 tgagattgag ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa
     6241 tctgcaattc acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct
     6301 ggcttaacgc catttgttga gcgatgtcag aagcaatgta acgctgccct gaatagacag
     6361 aacggatcgc actcacaact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac
     6421 cggcctgcat gactttcgct ggtaaagggt tttctgtatg gacggtaagc atgatgattt
     6481 taacatcagc tgtggaacgc gcgattttac gcgtcgcctc aaggccgcca atgcccggca
     6541 tactcatgtc cattagcacc acgtcaacgg catttgcccg gcaccattta acggcgtctt
     6601 caccgcacga tgcctctccg acgactttta taccctttat atcttccaga atgcgtcgta
     6661 tccctgcgcg caccagttcg tggtcatcaa caagtagaac gttgatcaaa ggaatatctc
     6721 cagaaatagg gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca
     6781 actttgaaac gttaaaaatg gtgggtattc gatttttctc tcgtttttgt aaattcgatt
     6841 gtccacagtt atggtcacgc ccgtcaccaa cggcttccgg gatcgtttca ctgtaagccc
     6901 tgcgatgaaa tgtgacaaaa attgacttta ttcagcaaaa atgaaaatca gcccgctgaa
     6961 tatgtaacat taattaaccc gaacaatacg ggcaaaaaac attc
//
LOCUS       DAZGAP010000224         6997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGAP010000224 DAZGAP010000000
VERSION     DAZGAP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.225"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..836
                     /gene="gadX"
                     /locus_tag="ACHLAM_004577"
     CDS             12..836
                     /gene="gadX"
                     /locus_tag="ACHLAM_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO7856006.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1205..1933
                     /gene="gadW"
                     /locus_tag="ACHLAM_004578"
     CDS             1205..1933
                     /gene="gadW"
                     /locus_tag="ACHLAM_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO7856007.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(2296..5409)
                     /gene="mdtF"
                     /locus_tag="ACHLAM_004579"
     CDS             complement(2296..5409)
                     /gene="mdtF"
                     /locus_tag="ACHLAM_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO7856008.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGDHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5434..6543)
                     /gene="mdtE"
                     /locus_tag="ACHLAM_004580"
     CDS             complement(5434..6543)
                     /gene="mdtE"
                     /locus_tag="ACHLAM_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO7856009.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSESPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6651..6929
                     /locus_tag="ACHLAM_004581"
     CDS             6651..6929
                     /locus_tag="ACHLAM_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856010.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6930..>6997)
                     /locus_tag="ACHLAM_004582"
     CDS             complement(6930..>6997)
                     /locus_tag="ACHLAM_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO7856011.1"
                     /translation="MYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1771 a         1686 c         1727 g         1813 t
ORIGIN      
        1 ttaagtcaac tatgcaacca ctacatggta attgtctaat tgcgtacgca agacataaat
       61 atattctcac catggttaat ggtgaatatc gctattttaa tggcggtgat ctggtttttg
      121 cggatgcaag ccaaattcaa gtggataagt gtgttgagaa tttcgtatta gtttcaagag
      181 acacgctttc attatttctc ccgatgctca aggaggaggc attaaaactt catgtacata
      241 aaaaagttcc ttcattactc gttcatcact gtaccagaga tattcctgtt tttcaggaag
      301 ttgcgcaact atcgcagaat aagaatcttc gctatgcaga aatgctacgt aaaagggcat
      361 taatctttgc gttgttatct gtttttcttg aggatacgca gtttataccg ttacttctga
      421 acgtgttgca accgaatatg cgaacacgag tttgtacggt catcaataat aatatcgccc
      481 atgagtggac attagcccga attgctagcg aattgttgat gagtccaagc ctgttaaaga
      541 aaaaattgcg cgaagaaggt acgtcatatt cacagttgct tactgagtgt aggatgcagc
      601 gtgctttaca acttattgtt atatatgggg tttcaattaa gcgagttgca gtatcctgtg
      661 gatatcacag cgtgtcgtat ttcatttacg tttttcgaaa ttattatggg atgacgccca
      721 cagagtatca ggagcgatcg gcgcaggaat taccgaactg tggctcggcg gcaagtatgg
      781 ctgcgcaagg gattttttac ggcactgacc gttctgcgga aggaataaga ttatagagtt
      841 ttactcagac ataaaaaaaa cccggcatag gggaccggga agaggatagt ctgccgtctc
      901 cagactaata aaccgttata acactccctg ttggcacggg aaactttgtg ctctcagtaa
      961 gttaaatata acttttactg gaaataagat cagccatttt tttataaaca taagctatac
     1021 gctgtgtgaa aatataatcg tgttgcattt acttattatc aattaactgt tatgcaaaac
     1081 tactttgtgg ataaaatttg gtcctaccaa atatggcagt ttttgcacac agaaacagtc
     1141 tggcgtcatt tcattagtat actgacattg aaataatcgc agtaatgaaa tataagggat
     1201 agtcatggct catgtctgct cggtgatcct cgttcgtcgt tcattcgata ttcatcatga
     1261 acagcaaaaa atatcgttgc ataacgagag tatcctgctg ctggataaaa atttggcaga
     1321 cgattttgcg ttttgttcac tggatacgcg acggctggat atcgaagagc tgacagtttg
     1381 ccattactta caaaatattc gtcagttgcc acgcaattta ggattgcata gcaaagaccg
     1441 tttgttaatt aaccagtcac cccccataca gctggtgacg gcgatttttg atagtttcaa
     1501 tgacccccgg gtcaattcgc cgatactgag caaaatgctc tatctttcct gtttatcaat
     1561 gttttctcat aagaaagaac tgatcccctt acttttcaat agtatcagta ctgtttcagg
     1621 aaaagttgaa cgccttatta gctttgatat cgctaaacgt tggtatctac gcgatatcgc
     1681 agaaagaatg tacaccagcg agagtctcat caaaaaaaag ttgcaggatg aaaatacctg
     1741 tttcagtaaa atattactcg cctccaggat gtcgatggcc agacgattac tcgagttacg
     1801 tcaaatacct ctgcatacta ttgcggaaaa atgtggctat agcagtacgt catactttat
     1861 aaatacattt agacaatatt atggtgtaac gccacatcag ttttcgcaac attcgccggg
     1921 taccttttcc tgacatattt cgcatttgaa tattggtcag gatctcacgt ctgcttcatg
     1981 tgaaactctt tcctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc
     2041 aggcggtata ggtattttca ggaggaaaag actcggcatg tttgggatta ttaagctaac
     2101 aattcatacg attacgggaa tgtgggtcag tattgtgctg ttcaaattga tgacgaatgg
     2161 ctggagtggt ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg
     2221 gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct gaaccctcac gttcaacctt
     2281 actctcattt acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa
     2341 aaagaccgga acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccattacgcc
     2401 ggtacctacc gcgttttgcg cgccagagcc ggcaccatga ctgataacca gcggcagcac
     2461 gccgagaata aaggccagag aagtcatcag gattgggcgt aaacgcatac gcgctgcttc
     2521 gatgattgcc tctaccggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac
     2581 aatcaggatg gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta
     2641 aacgtcatta cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg
     2701 aacaaccaac atcaccgaga acggaattga ccagctttca taaagtgcgg cgagggcgag
     2761 gaacaccacg accagtgaaa tggcatatag cgcgggagcc tggtttgagg ataacgcttc
     2821 ctgatacgac agcccggtcc atgagtagcc gacgcctgcc ggaagttttg cgaccaggtc
     2881 tgccataaat ttcatggcgt caccggtact tttcccggca gccgcttcac ctaaaatctc
     2941 cattgacggg atgccgttgt agcgttccag tcgcggtgaa ccataggtcc attcggtaga
     3001 cgaataggca gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat
     3061 gttatccggc aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc
     3121 ctggttgagg aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat
     3181 atcagacagc gccacaccca tagcctcggc tttcgcagcg ttgacgttca ctttgaacat
     3241 cggcgtatct tccaggccgt tcgggcgtac cccgatgacc tgattcggtg attgcgctgc
     3301 cagtgataac agctcgtttc gcgcctgggt cagcttttcg tgccccagat tgccgttgtc
     3361 cagcagttcc atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa
     3421 cgggaagacg acggctttat tgatactgct tagcgcaatc attgcccgct gaataatcgc
     3481 ggtaaccgag ttttcctcgc cgacacgttc agaccacggc ttgaggctga taaacgccag
     3541 accgttgttt tgcccctgac cactgaagcc aaagccacca acggtaaaca ccgactgaac
     3601 attatttttc tcttttgtca gataataatc cgtcacctgt tgtagcactt tcgtggtgtt
     3661 aaccatggtt gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc
     3721 tggtaagaaa gaggtcggcg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag
     3781 gtagaccacc atgtagcgac cggtacaacg caacagcgag cgggtgctgt cggtatagtg
     3841 ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgatc
     3901 gccctccggc gcggctttca gaatggtggc gcacagggca ggggtcaggc tcattgctac
     3961 aaatactgaa agcagcatgg aggagatcag cgtgatggag aactggcggt agatctcccc
     4021 ggtcgcaccg ctcataaagg ccatcggcat aaacactgcg gaaagaacaa cggcaatccc
     4081 gaccagcgca cgttggatct gccccatcga tttatgcgtc gcttccttcg gcggtagctt
     4141 atcttccgca atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa
     4201 ccctatcgcc agcaccatcc cgaacatagt taacgtgttg atggtgaaac cgaccgccga
     4261 caagatcgca aacgtcccga gaataaccac cggtacggca atcgtcggga tgattgtggc
     4321 gcggaaattc tgcaaaaaca gatacatgac caggaagacg aggatgatag cctcaaccag
     4381 cgttttgaaa acttcctgaa tagaaatttt gataaacggt gtggtgtcgt aaggataaac
     4441 cgtcttcagg cttgcaggga aataagccga taaacggttc agctcctctt tcaccgctcg
     4501 cgaggtatcc agggcgtttg ctccggtagc cagtttgatg gcgatcccgg cagcaggttt
     4561 gccgttatag cgcgccacgg tggaataatc ttccgcccca agttcgacgc gggcgacatc
     4621 acgcagcagt acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc
     4681 cggcgtttgc agacgcgtct gcacaatgat cgaggcgttc agttgctggt ctgccgcctg
     4741 tggcatgcca cccagttgac caccggaaat ctggttattt tgcaccttaa tctgggaaat
     4801 aacatcggaa ggtaccaggt tatatttatt gagtttttgc gggtccagcc agatacgcat
     4861 ggcgtactcg gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc
     4921 tttgatatta gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga
     4981 aataaatgcc gctaccatca ggatattact gctcgactta tcgacgctaa tcccctgctg
     5041 ctgcaccgct tcaggtaatg aaggcatagc gagctgcagt ttattttgca cctgaacctg
     5101 tgcgatatca ggagatgtcc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc
     5161 atcactggtt gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac
     5221 ctgagtgacc gagtcttcta ccgtttgcgc atcggcaccc ggataggttg cgctgacggt
     5281 aatggtcggt ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc
     5341 acctgcaagc atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata
     5401 gttagccatg aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt
     5461 ttcctggctg gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga
     5521 aacgatcacc cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc
     5581 tttgctggct tcaatttcgc gtagctgcac aacatcgtct ttatccagaa tgagcgccgt
     5641 tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc accagtaata cattctggcg
     5701 gctaccttca tccactaatg ccgtgacgta catgccaggc agcaagtcac catttgggtt
     5761 ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca tccaccgtcg ggtcggagaa
     5821 tttcagcgtg ccggtctggc tgtaacgttt accattttcc agattgagct gtactggcgt
     5881 actgccctga acctgtttga tttgcccact ggcgacctct tctttcatgc gcaagaaatc
     5941 ctgcaccgac tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga
     6001 atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc
     6061 cgtaatcggc gaggtgacat ttgcgtattg caggttgata gtcgcctgtt caacagccgc
     6121 tttggcgacg gtgacattgg cttctgcttc attcaactgg gtgcgcgcgg tgtcgtaatc
     6181 ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc
     6241 attgctggcg gtagagagcg ctttcgccag cgagcctttg gcggagttca gctctgcctg
     6301 taaaggcgca ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat
     6361 aaagttgcgt ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg
     6421 aacggttcta ccaggcgatt cgctcaacac attgaccgaa ccgggggaga gtgtgacgac
     6481 accgacctca ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag
     6541 catcgcgccg cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc
     6601 tgaaaattct tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt
     6661 tacagaccag cggcatagtg gggttgtcag ccagtaaggt gggatacagg cacagtgatc
     6721 gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca
     6781 ttcgggcaac gaagggttct actggtggat acatatacca ggggaataaa gtcgttttcg
     6841 cctctccttt agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca
     6901 gccatcaatt tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg
     6961 atgtcgctca tacgtcttag tttcagtttg tacataa
//
LOCUS       DAZGAP010000225         6981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGAP010000225 DAZGAP010000000
VERSION     DAZGAP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.226"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(143..820)
                     /gene="tli1"
                     /locus_tag="ACHLAM_004583"
     CDS             complement(143..820)
                     /gene="tli1"
                     /locus_tag="ACHLAM_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO7856012.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKNLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMKMCKGVTKSDFSYGYDEDIKEFIRDK
                     TYPYGNW"
     gene            complement(840..2522)
                     /gene="tle1"
                     /locus_tag="ACHLAM_004584"
     CDS             complement(840..2522)
                     /gene="tle1"
                     /locus_tag="ACHLAM_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFO7856013.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(2519..5038)
                     /locus_tag="ACHLAM_004585"
     CDS             complement(2519..5038)
                     /locus_tag="ACHLAM_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7856014.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(5117..5344)
                     /locus_tag="ACHLAM_004586"
     CDS             complement(5117..5344)
                     /locus_tag="ACHLAM_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856015.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            complement(5341..5982)
                     /locus_tag="ACHLAM_004587"
     CDS             complement(5341..5982)
                     /locus_tag="ACHLAM_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856016.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTRHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(5969..>6981)
                     /locus_tag="ACHLAM_004588"
     CDS             complement(5969..>6981)
                     /locus_tag="ACHLAM_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7856017.1"
                     /translation="TGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAA
                     NTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQ
                     LKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQMFDRGFMRDGE
                     GREIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLARFQ
                     TVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARN
                     VESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWNDEDGIVIAFDGINSDYPQESIYG
                     QC"
BASE COUNT         1822 a         1878 c         1387 g         1894 t
ORIGIN      
        1 tattgtcatc ctgtagcgtt aaacaccaga catcataact ttagtatttt ttactgcgtt
       61 atcattcagc tattgaccgt ttttttacgc cagatgaaaa ttagactttc tttttacata
      121 ctcatcatta ttctggctta agtcaccagt taccgtaagg ataagtttta tctctgataa
      181 actccttaat atcttcatca tatccatatg agaaatcact tttagtaact cccttacaca
      241 tcttcatttt atcacccgat actgtagtaa ttttagcatc caactgaggt accaccggat
      301 taccgcgatc tcccagccag actcttacct tcccttcagg tgccagacca ataatcatcg
      361 ttgaacgata ataggtttcg ttccagcggt tgtctgtgta aggcgtaacc atctgctccc
      421 aggtcccttt tttaaaaaaa atcagagttt cataactctt tttatcaatt attgaatccc
      481 agcaaaaacg taacatcaga ggtaatgctt tccccttgtt ataatacgac tcagtcgcgc
      541 ccagatggtc attccagcgc cctacactga cctgactcgg tcttgtggta tcaagatact
      601 gataagtcgt cttataacct tccccgtcca ccatcaatgc ctgagtggcc agcgcaggca
      661 gagcctttgg ataaataaag agaaatttcc attcatcata gggtaaattt ttataacccc
      721 attcagtctt atccgaatca gcatactgtg agctaaccga attacaccca gttaacaatg
      781 ccagtaatgg tgcgataaaa atgattttat ctctggtcat atattgccac cctgtggtgt
      841 taaacaccag acatcataac tttagtattt tttactgcgt tatcattcaa tcgctgctga
      901 tcatccatgt accatacaga acgaatccag cgatcatttg gtttattcgg aaaaacgaac
      961 gccttattac ctttaggacc aaagcgatgg ataaaaatct ctcccgttgt aggatccacc
     1021 cataaattac catcagactc aatattccag ttggcagaac aatgggtgta ttttccgata
     1081 atatacaact cttcctcagt aaaagcctga ggctcttgcc ccaaacgtac cgcttttccc
     1141 tgagcaatgg ctttgtcagc aagaaatatt agttcagatg gaagttgtaa ctcagtattt
     1201 gtttgtcgaa ttggttcaaa caatacccct gcttcctgag cagcatccag catcacacgc
     1261 aggcaaacat tagcccagtc attaggtact gccatacgct caaaaaatac tgcggctccc
     1321 acctgctttt gtatcacacc agcccgacgt tgattgctgt tgttcacacc tacggaacga
     1381 attttggtgg ttagttttcc atgaggcaaa atatgtttta aggctggcag agtcatcaac
     1441 atttttctgg cttgctctgc attacggtat actcgcgtat ccgtttctct ggtatcgtca
     1501 ctgacaattt caaattccgg gcaactcaga aacagacttt cattttcctg cagaggtgaa
     1561 cctaccggat tgtaaccacc tccaatatct gaatgcgccc ctggtaaagc cagttcgggc
     1621 cacatacctt taatactgtt caggctgaaa ttatatcggt attcattcat tgcagtaatc
     1681 tggaaaactt tcttcacgac tgaaggacgc aattccagct ttactcccgg gttgctacgt
     1741 ccattaatat cgaagaaatt actgattcca ccaatagctg cgacagtatc aaaaagcccc
     1801 agaaaacgga cttcaccaga aggtttacca tcataaaaat cgcctctgag ccctttagca
     1861 atagctctgg ctatagcagg atcctgttcc ataacccgat tggcaaagtg ccttgccgcc
     1921 gctgctcccc ggctaaaacc aaaaacatca aactggattt ttgcgataca aaaatcagga
     1981 tttaaattga accccataaa ttctgataac gcctgggtga ttcgttccat tgcctcgtcg
     2041 gttttcgtca ccacaccttc aaatatatcc agaatactgg tgcctaatcc catgccaatg
     2101 actgaatctg cttcaccagc cgcagtccct atcccggaaa tgtaagtctg aatttgtgct
     2161 tcaacctgac tttccaacaa agtctgatct gctacgtaaa gtgaatttag ccagtgaata
     2221 ttggaataat atcctcgata actgccattc gagatactgt tcttgttaat ttcttcaaga
     2281 aagtcctcac agctttgtgc atctttttga tttaccccct gtctggctgc actacactca
     2341 ttaaatttca tcaggcgaga tgctgtattc tcccgattgt ttccggtacc atcgaaaaat
     2401 ataccaatgg ttaaagtcac accccgcgcc ggttttttct ttgcaacaac aactttccag
     2461 tcattatgaa tactggtttt atgggtggca tctgttacgt catatccctg atattttgtc
     2521 attctgtttc tccatgaatt tttaccttcc caaactcaac ttttactact gacggaatgg
     2581 aggtgttgac tggcgctgtt ttaccatttt catcggtgat cccttgtaga atatcacctt
     2641 cagccgttgt taatcgataa ggcgtcgaag gttgcggcac tcccgcttca tcggtaagaa
     2701 taaaaccgcc accaaaccct cgcggcattt caacagacgc aatatcaaga cttgttggtc
     2761 ccatcttctg catattcgtg ctttttaaca ggatctgtcc cgggcatccc agctcaatat
     2821 ttccaccgct tagtttgata taagctccac cacatgccag tatcagttcc tgattagcag
     2881 aaacagtcac tttgccctca gaactttgaa tatcaagatt ctgttgggaa ctcattgaca
     2941 aatcgtcact ttgagcctga atatttaccc gcccttttgc tgctaacagt tgcatcccct
     3001 ggtttctgct caacaggcta acagcacctt ctgctacaac tgtaaatgac tgcccggcac
     3061 taatatcagt gttatctccc gacattatgc ccacgctttc actacccgaa gcaattcgta
     3121 acgcccgtgc actaaccatg ccaatcccat caggagcatg caacagcatt cccggcgcag
     3181 tgagatctgt tagcgatgaa ttcaaccgtt gctgacaact tatatctccc tgagtagcat
     3241 ttgcagcagt tgcggccttc gccatacttc gggccagaga taatgcctgc tccagttggc
     3301 ggatagcttc attcatatcc agcatctttc cttgtgcctg aggctgcata tctgcactga
     3361 taaatattcc ttttcctccc cggatactta tccagtcatc agtcctcagc tcagcgcctt
     3421 ctcccctgag tttccgggag gcattaacgt tatgaccaag attaagttgt gtcttaccgc
     3481 caaactcggt actgatcttg atatgctcct caccgcgttt gtcctccatc cgcaacttgt
     3541 tatttgccgg cgtacggata acattgcgcg taccgttctt atcggtaacg tgatcaacat
     3601 gacgtgaatc atgcagggca tgcgcgatat aggggcgatc cggatcacct tcctcaaagg
     3661 caatggccac ttccgttccc tgaatcagcg gaaaatgaaa accataagca tcaccactgt
     3721 agggtttggc cagacgtacc ggcatgcttt cctgtcccgg atttttatca tcacggtcag
     3781 catcaaattt cacccggtac atacctgatg catcctgcca ggcgtagata tccccttctt
     3841 ttgcactggt cacacgggcc gtcatggtac cggtaacttt cggacgcggc agtaaaggag
     3901 ggcgccagca gataacttca ctgtaaggaa cgcccttcag tacaacctgc agggcatcct
     3961 tgcgacttgc agaaaaaccg acacaggtaa gcaacaccgg attgcgcagc agcgcaggca
     4021 acgtcggcgg aagactgtcg gtgatagtca gaacctgagc aggtgccagc gtggcgtctg
     4081 tactgttgcc ggttatccgc acctgctccg ccagataacg ttcatgacca aggcgggcat
     4141 aaaaattggc cgtctccggc accgggtcga ttttatcccc ccgttccaga tgacggaaat
     4201 ggtagtggta cacctcacca tagttaattc cgtcaccatc accatgcgtc atgtctgccg
     4261 gggcggactg caacagatat tgtgcttcac gatggttata agcctttgtg gtgacactgg
     4321 cctctgccac attgtgcgtc acattcagcc cccacactga atccgctctg ttatcattca
     4381 ttccggacgg actgtttatc gccagcgtct tatcaaacgt cagcgcagcc tggctgtcac
     4441 caaaatgcac tacttccgtc tgcgtgtccg gttgcagggt gaaaaaataa aaaatcccca
     4501 cttcagccag caaacgctga ataaattgca ggtcactttc ctgatactgg ttaatctgtt
     4561 cacgcttcgg atacgttttt ttgagacgga actcaaactc ccagcctttc aggccgtgct
     4621 cctggagaac ctctgtcacc acctccggca ccgatttgtt aacgaaaaat cggtgagaac
     4681 gatgctgcct gtccagtagt ttaataaatg gttccagtat cacctggtat tttgcctggt
     4741 cggcggagcc cgatatacgg cgaaaatctg taatgacacc atgtactact ttctggctca
     4801 cctgctcctt aagccctccc gtacccattg taaatgtggc tgatttacgc agcagcagcc
     4861 cggcatccag gtcgttgtca gtactggtaa aattgatgat gtaataatag gtctgactca
     4921 tctcctccct gccgctgaaa tgctcaacat ccagtggtga cgggcaggag ggaataatta
     4981 aatggtaacg attcagggaa gcagagatag tttttgaaag tgtatctgtc agactcatat
     5041 tgttaactcc atgtctgtac cggtattgaa ctgcaattat ataatgatga ccgttaagct
     5101 ttattttcag agagaattac tgataatcat cccattcatc gtaaatatat gcatatgcaa
     5161 atcgatacat cagataaccg acaaaaaata acgcaacaga gagccctccc cacagatatt
     5221 taaattcagc agatgagaga aaaaagaacc agactgtaaa tacaataccc gacgcaccta
     5281 accccatacc tgcgccacgg ataaatcggg aaagcattat aagtaatgtt ctttttatct
     5341 tcataaacca ccgaaaaaat attcgcatat ccggtcaaac tcagccgaat ttcggtctgc
     5401 gacaatacag gctcgttcaa acgggcgcac aatatcttca acaagaaagt acaaaagatc
     5461 caaatccccc tgagctttca gcttatcatg caacaccgga tttctgtctt tcagaaaacg
     5521 ggatgtgtaa atcgcacgcg atgtctctgc gccaacagat aataacaacc cggcaaaact
     5581 ccctgcaaaa aaagcagcca gccctctgga taccagaacc tctcccagtt taaccccggt
     5641 caattcagca aatgccattc tacctgccag atctcctgaa acgttcttca caatggagcc
     5701 tgttttttcc atattgatcc gggatacaaa gtcttcaaga caaatactga taattttccc
     5761 aataatatca cggctgaaaa tgcctttttc taccagtctg gccattctga atttgtcatt
     5821 ctggttttcc cgcctatgcc tggtatctat aaaatcatat cccatataac cgagactgac
     5881 tggcaaagat atgagtccct tcccgacggc ccccagaaac ccatccccat taatatttac
     5941 ggcctccagc atatcccggg caatttcttt agcactgtcc atatatgctt tcctgcggat
     6001 aatcgctatt gattccatca aacgctatca caataccatc ctcatcattc cacgtcagag
     6061 acaggtacgt cggatgctgt ctggcagcaa gatagctgag caactgccgg ctcagtaccg
     6121 gcagcagttg ctggttcagc agactctcca cattccgggc acccgtatcc ggcagcaggc
     6181 aggcagatgt cagggcatcg tacacacgtt catcaaccga tgtgctcagt ccataatggc
     6241 agtgcaggcg ttcacagacc tgtgcaagct tcatctccac aatgatgcgc atggcagact
     6301 gcgtcagcgg acggtaaatc acggtctgga aacgggcaag cagtgctggc tggaagtggt
     6361 cgcgcagcag tggacggata agctcatgca gctccgattc cgttgtctcc ggcttttcgc
     6421 tcagttgctg catcagaagg tcactgccca gattggaggt catcagaatg accgtattac
     6481 ggaagtcaat ttcacgcccc tcaccgtcgc gcataaagcc ccggtcaaac atctggtaga
     6541 acagattcag cacatcccgg tgggcctttt ccacttcatc cagcagcacc acgctgtaag
     6601 ggcgtttacg cacagcctcc gtcagaatac cgccctgacc gtatcccacg tacccgggcg
     6661 gtgacccctt aagctgggaa accgtatggg gctcctggta ttccgacaga ttgatggtaa
     6721 tcagtgattt ttcgccgccg tacatcacat ctgccagcgc cagtgccgtt tcggtttttc
     6781 ccacaccact gggaccgacc agcaggaaca cgccctgggg gccgttaccg gatgtcagac
     6841 ccgtattcgc cgcacgcaga cgctgcgcaa tggattccag tgccacgtcc tgtccgacca
     6901 cccgtctgcc gatatccttt tccagatgca gcagttccgt ctgttcgtcc ttcatcaggg
     6961 aagataacgg caccccggtc c
//
LOCUS       DAZGAP010000226         6959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGAP010000226 DAZGAP010000000
VERSION     DAZGAP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.227"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(15..2798)
                     /gene="pqqL"
                     /locus_tag="ACHLAM_004589"
     CDS             complement(15..2798)
                     /gene="pqqL"
                     /locus_tag="ACHLAM_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7856018.1"
                     /translation="MRNLCFLLMLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPSNKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNAQDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLLPLITLYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2855..5227)
                     /gene="yddB"
                     /locus_tag="ACHLAM_004590"
     CDS             complement(2855..5227)
                     /gene="yddB"
                     /locus_tag="ACHLAM_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO7856019.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDVNPQ
                     IVFSYIKSKGNLSLNNGYEESNTDDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYINEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5265..6950)
                     /gene="yddA"
                     /locus_tag="ACHLAM_004591"
     CDS             complement(5265..6950)
                     /gene="yddA"
                     /locus_tag="ACHLAM_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO7856020.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAEYLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
BASE COUNT         1799 a         1597 c         1569 g         1994 t
ORIGIN      
        1 tgacggggct gttattattt tggcaataat actccggtat aggtattcac cggatgagaa
       61 agatattgtt taacggcagt gttaacattc tctaccgtca tttgtttcaa taattgctcc
      121 tgctcagtcc atgctgcagg atcgtcatat tgaataagac tatttacaat ggtgttcgct
      181 aattgttgaa cgctacgctg ttggatatcg aggctgcgct gaacgttttg ctggtattcg
      241 ttcagttctt gctcactgat ccctttagcc agacgcttaa ccatcacttc attcgctaac
      301 gttaacagtt catcatgtcg ttccggttga caagtaaaag ccagcaaatg actgatatct
      361 ttggcctgag gatcaaccga gaggcgagaa gaaacgctgt atgccccaga ggcctgttca
      421 cgaatattaa cgcgtagatc ttttgccagt gcgacgttaa aagcatcgag cgccatacgc
      481 gtctccagat taaccggtgt ccgggaatca taacgcttcc actgtgaaac ctgtgccaca
      541 ggttcatttt gttcttttac agtaaccgat gcgttgtccg tcgcgcgagt taatggttta
      601 cctgcggcta atggcgaatc agagtgtttg attgatccta agtaaagcgt aattaacggc
      661 aggagtttgt cttctgcgac attaccgaca atgacaaacg tgatatccgc tggagatgaa
      721 aacaactggc gatcggcagc cagcgcatct gcggcagtaa actgtgcaat ctgattttct
      781 tgcagtaatt tcgtgcggtc atcagcatag cgcgtctcat acatctgctg ggcgaatttc
      841 tccgctggac gctggtcgag cgttttcaac gcctgaattt gagcattttg tagcgatgcc
      901 caaatattat cgttaatcgt gctgtgggtg attcgctggt taatcaactg gaaaccaggt
      961 tcagggttat tagtccgcgc gctaacagag agcaatgtat tcatgccact gactttactg
     1021 ctcatggtta ccgagttttc cgcactccag cgtttcaggc tggaagagga gagttcacca
     1081 acgccgcttc cgcttactgc tttatttgcc agcgcgataa gtgatttttg ctgcgcaggg
     1141 aaacttaaat cgcctttatt agatacggca gtaatttgta gcttttgctc ttcacccgcg
     1201 gattttgcca gaataaccct ggcaccattg gaaagtgtta atgatgtcag attctcagcc
     1261 agcgtttctt tgctgctaat ttccgcctgt gggtcagcgt ctactgttaa cgataaattt
     1321 ctgcctggga agatgtaagc cgccagcttt ttgttggcgt actctttttc cagcgccagg
     1381 atagccgcag gagacaatgc ttttttggcg gcaacttcat tgtttaccat ttgctcccaa
     1441 aatgcgtcct ggttctttct taactgctgc catttttccg ccagactttg cacggtaatt
     1501 tgctgccaca gacgtttcga aagttgatat gtctcttccg gcgacaagaa cggcgtatta
     1561 tttaatgagc tggatgccag gcgactggtc agcatacgta aatcacgctc agcttgctga
     1621 tcaaccgcat ttttcagcca ggtaaggcgg gtagatttaa catcatcgag ttcttcagca
     1681 gaaaagccat gctgatcaat ggttgccaac tctgccatta atgcattcgc agcatcctgc
     1741 atattatcgt cttgtgcatt tacacggaaa aacaaagact gataatcggg tgcaattttg
     1801 acgctgcgcg cagtgccgcc agaaatagtc ttcaactcgc ccgactgtat gcgttcttgc
     1861 agacgttggt tgaacagctg aaccaacatg ctccattcag cttgttcgat aaagctttgc
     1921 tcatcgttca cttgtaccat tggcaggcga taatagagtg cgatgccgtt cacccggttt
     1981 tctttatcat tgatgatatt aaagcgcagg tggttttcgg cttttgtcgg ccagacgcga
     2041 ttttcagctg ctttgttaga cggaagctta cttaaattat ccttaatcag cgccagcgct
     2101 tctttactgt caatatcgcc gaccacgata aaggtcatat tatttggttg ataccagcgt
     2161 tgataaaatt ggcgcaattg tgccggtgtg accgtagcga cagtatccat caggccgata
     2221 ggttcacggt ctaaattacg ggtatttgcc agcaggaaag ggcggcgcgc ctgagaggtg
     2281 cgccatttcg catcctgatg ggcacgccat tcctcagtaa ttacgccgcg ttcagcgtct
     2341 acttcgagtt tttcaaaggt tgcggcatta ctccattcac tgaagattgc catcacttgt
     2401 tgcagatttt gtttctgcgt agtcggcaaa ctcacctgat acactgtttc gtcatagctg
     2461 gtataggcat taacatcgcg accaaaacgc aggcccattg actcaaatgt ttcgatgact
     2521 ttattacccg gccatgtttt tgtgccgtta aacatcatat gttctacaaa atgagccacg
     2581 ccgcgctcat tgtcctcttc ctgcaatgaa ccggtatgaa tttgcagcca taaatttacc
     2641 tgatcctttg gttgagcatg cggataaatc atatatcgca agccattgtc cagttgcccg
     2701 gtaattaact tttcatcctg cggtaaggcg gcggcaatca gtcgcccggg gagcaacaga
     2761 gttgccacta acatcagtaa gaaacagagg tttctcataa ttatctccat gcgaaaaccg
     2821 ggcaaattta cccggtaaag taaaatccga actattaaaa tttcatgctg acatcaagcc
     2881 agaaagtacg gccactggcg taggtcgcca cgccggtatt cgttgtatca accgcggttt
     2941 tgctatccag tacattgaga atatcggcac tgatcgtcag gttttgttgt ttcagaaatt
     3001 gtggcgtcca ggacaagcgg gtgtcccagg tcaggctgct atccagtttc tcgtcaacat
     3061 actgcttgta atctgaatat tcgttgatgt attgcgcatt cgtcttaccc aggataatgc
     3121 gagctttacg cgcttcttgc caggtcagcg tattcgccca caccaacccg ctcggttgat
     3181 gcgtgaaatc catgtttaag gagatcttta atggattatt aaaatccgcc actggaacgc
     3241 tatcgtaaga gaccagatta ccgttataaa ccacccggtt atcatcggta ttgctttcct
     3301 cataaccatt gtttaacgac aagttgccct tgctcttgat atagctaaag acaatttgcg
     3361 ggttaacatc tacctggctg atatgcaggg gttcggccag ttcaaaactg aggttgaacg
     3421 aatgcgtttt ggttttgccg tcgttgttat attcagtaat ggtggtttta gtcgcgctgt
     3481 cggtacgact gcttttgctg atttgatcat gcgcttcacg gtaaacatag tttgcgcgcg
     3541 caataacgtt cttaccgatt ttctgctgca accccattgc cagttcatcg ttataaggcg
     3601 ttttcaaatc ctgataacgc gtcagggttt tattgcctga taccgattcc gtccagctat
     3661 tgcggatatc acgtaatccc atatcaagaa tattcccgcc atagtaacgg ttataacctg
     3721 cggtaatcat tgaggtttga tcagcaaaaa tatcccattc cgtcataaag cgcggggaga
     3781 tattgtggtt tgacagatag ttgtcatagt cataccgcac accgggcatt aacgacacat
     3841 tccgccagct aatgcgatcc gccatataca gcgtgtagtt gtcaattccc agactgcctt
     3901 tacctttatg gtaaatggta tggttagttt tctttcctgc agcattaatc acataggatt
     3961 cagactggtt atggcgttca gtccatgcat cggaatagat gtattccgcg ccgaagtagg
     4021 gttgatgcga aacatcaccc acggcgaatt tttgccagtc caggcgtgtt ttgaaggtgt
     4081 aattatctac agcctgggaa atgtgtccta atccaccacg ggtgcaacga cctgaaatat
     4141 caccatatgt acatgaaagt tcggtgtacc agatgtcatg atcgtgacgg gtataatcac
     4201 taatatgatc ccaaccaacg gtagtacgta gtttggccca tgcgagctgc gtatccatat
     4261 cccatgccag accgtaggat ttattaccca tttcgcgatc agactgcggg aaggtgctgg
     4321 tattataatc acgactggag ccggtatatt ttaaggtaaa atcgtgggta aagcggtcgc
     4381 tggcaaacca agtaaatttg ctcaatgcgg tatcgataac gtttttatac tgtgcccgac
     4441 cggcgacaat gccgtcattc gaaacataat ccgcgcgggt gatatcagac tggcggcgcg
     4501 ataaaccggc ggtaacgcca aagttatcag cgagttcctg attaaacgac aaggtataaa
     4561 agttcttttt aaaatctgga gagtaataag tacttcctga agaaccttga ttaaatgcgc
     4621 tcttgttatt ctcatcgata tgcgatgtta accagtccga acgcgtagtg cgataaccca
     4681 gtttcacgct gctatcatca gcgttgaagc gtttgatctt tgcatcaatt accccgccat
     4741 tgaagcgacc gaattcaacc ggcacaaaac tgtcataaag cgtcacattg tccagtaagc
     4801 tgacatcaag ataatagccc tgtgacatcc cgctaatatt ggttgcacta ctggcatcgg
     4861 actcattcgc tgggttcagg ttattagttg cactaatacc atcaatcaaa taggcattct
     4921 ggtagggcga cgcaccgtga atagagattt tctccgggcg aatatctccc tggttcaatg
     4981 aggtactttg cgttgaatcc atgcgcaccg cagggttggt tctcagcaga tcgctgatat
     5041 tgccattccc ggtcggtaaa cgctcgatgc tttcactggt gtaatgcgtg ttgccgttta
     5101 cgggtacagg gacaggagca tagaccgtca tctcttcaaa aacatgcatg tacatgtttt
     5161 tgtcatcgac ggcctgcgct acatcagcgc cacataaaat gacgccagga ataagaactc
     5221 gcttcattta tttatatctc cgtacagcag ggcttttatt gtttttatat aaccgcgcta
     5281 atgtcacaaa tatcatcagc caggttccag acaccgggtt gatgtgtaac cataatgacg
     5341 ccgcttgtgg gtagtttttc acgcactaaa cgcagtaagc ggatagcctc ttgttcctca
     5401 agatgagagg tagtttcgtc aagaaatatc catttcggac gtcgtaaaat taatcgtgcc
     5461 agggcgatac gttgtttttc gccgctggaa agaatatcgc cccagcgatc gtggtcatga
     5521 atacgcgcag ccaatttccc cagaccaacc tgatgcagta cttcgctcaa cgatttatcg
     5581 tctacggaca ggggaagtgc tttacaaatt atctctttca gtaagccggt tttgattaac
     5641 ggtgtttgtg acacatacca actgtcagca ggagaagaaa tatcaccttt aaaccacggc
     5701 cagcagtggg ataatgtttt aagcagtgtg gtttttcccg caccagagta acctttcagt
     5761 aatagccatt tgcctggcga aacatgaaag ttcaggttct ctaatatgat cttattatca
     5821 ggagtacgaa tactcgcgtc agccacttgt accgcatgtt ggcaattttt aggcttattc
     5881 gtagggcgct gttcggtgag ttgatggaac tcatacaaac gatcgataac cgcagccagt
     5941 tcagcaagtt cgtcatattt ataaataaac cagcttaaat tgttcgatac cagcataaat
     6001 gcctggcgcg atttcatcag tccgcccaga ttgatctgtc cgctaataaa ctgcggcaat
     6061 aacagaaagt agggaagaac gctaagcgag cgcgaataaa tattctgcca gtaatcaagc
     6121 caccgctgac gattcattaa acgatgccag ttctctttaa tggtatgaaa attgtcgctt
     6181 aactcctggc gttgtagata ctcagcattg cttagcgcaa tcagttctgc ctgcttatta
     6241 tgctgcacaa gattagttcg aaatgtcgct tcgctacgtt gtttttccac attaagcgga
     6301 cgaatacgtt taccaacctt atgggtaaat aaagttccac cgatcacaat gagcacaacg
     6361 gtatagacca tatacccctg gatattccat tctgttccac caacagtgaa tgagagcgta
     6421 cccgcgcttt gccaaagaat aacggtaaag gtgatcaaca tgctaagtga ctggatgaag
     6481 ccaaatgaaa ggctgagcgt tttactgatc agtaagagaa tgtcctcagc gatacgttgg
     6541 tcagggttat ctgtattttt atgctcaccg tagatctgcg tgaagtagta gtttttatct
     6601 gcaaaccagc gattaaggta gtaatctgtt aaccattcgc gccagcgaat agtcagtagt
     6661 ttaattagcc atgttttgtt tacggatatt aatacaaaga tccccagcag ggcagggaac
     6721 caaaggacaa gctgccaaag cttatcggtt tctttctggc ttagcgcatt gaaaaaatca
     6781 ttattccagt cgtttaacca gacctgaatt ttaaccacgc caaggatcat ggcgaggata
     6841 ataatgatta acaacactga agttttatta ttcttacgca gccaaaatgg cttcagcaga
     6901 cagagatact ttgctatcaa catacaaaac gtaatgggaa tggttatcat tagcgaaaa
//
LOCUS       DAZGAP010000227         6941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGAP010000227 DAZGAP010000000
VERSION     DAZGAP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.228"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(50..283)
                     /locus_tag="ACHLAM_004592"
     CDS             complement(50..283)
                     /locus_tag="ACHLAM_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856021.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(378..1001)
                     /locus_tag="ACHLAM_004593"
     CDS             complement(378..1001)
                     /locus_tag="ACHLAM_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7856022.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKHFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNLTGTTRKIPAYHL"
     gene            complement(1090..1599)
                     /locus_tag="ACHLAM_004594"
     CDS             complement(1090..1599)
                     /locus_tag="ACHLAM_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856023.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQHEGAPIRCLLLLSQTSVCHP
                     RASVTVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLM
                     PRGIATIIR"
     gene            complement(1880..2058)
                     /locus_tag="ACHLAM_004595"
                     /pseudo
     CDS             complement(1880..2058)
                     /locus_tag="ACHLAM_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            complement(2126..2399)
                     /locus_tag="ACHLAM_004596"
                     /pseudo
     CDS             complement(2126..2399)
                     /locus_tag="ACHLAM_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2446..3012)
                     /locus_tag="ACHLAM_004597"
     CDS             complement(2446..3012)
                     /locus_tag="ACHLAM_004597"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7856024.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            complement(3040..3237)
                     /locus_tag="ACHLAM_004598"
     CDS             complement(3040..3237)
                     /locus_tag="ACHLAM_004598"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7856025.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
     gene            complement(3485..3910)
                     /locus_tag="ACHLAM_004599"
     CDS             complement(3485..3910)
                     /locus_tag="ACHLAM_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7856026.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDCGGAST"
     gene            complement(3907..4290)
                     /locus_tag="ACHLAM_004600"
     CDS             complement(3907..4290)
                     /locus_tag="ACHLAM_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7856027.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(4662..5255)
                     /locus_tag="ACHLAM_004601"
     CDS             complement(4662..5255)
                     /locus_tag="ACHLAM_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7856028.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRLDSTLSEICSLV"
     gene            complement(6487..>6941)
                     /locus_tag="ACHLAM_004602"
     CDS             complement(6487..>6941)
                     /locus_tag="ACHLAM_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856029.1"
                     /translation="NFTLMRVIKMYNSMLANISTKSDIQPPYITGLLDNVERALDKIN
                     ILIDKERVYDGETLAELITENKILSSRESKENLIGLFTGRHKYTLLQCIEKLGVLVDY
                     VISPADDIKNVMMLYGNNAENKNRRRLIYNALTIICGDDTKNNQSDPR"
BASE COUNT         1960 a         1735 c         1488 g         1758 t
ORIGIN      
        1 gaaccccctg ccggcaatca cactgcctga tggcagcata atccacttat taattctcat
       61 ccccggagac aagccctgaa aaccactcgg gatgttcact gtatgctgcg ttcagagcat
      121 cccaggaaac gttcatacag aagctgtcct gcaatttgca ggcaaccacc tctgccaggc
      181 ggttccggtt atccgccagt ccggcataac ctctctctgc cagccactca ttcagttcat
      241 gttccagaga cagattgata aaatcgtaat cggaccttgc cattcccgtt tccttaacac
      301 atgtcttcag actcagggat aaagtataaa aatgtcgccc tgctgctgcc agagcaatat
      361 tccccctgat ggccgtatca gagatggtat gccggtattt tacgggtggt tcccgtcagg
      421 ttatcgtgta cctccacgct cgtagtcacc accggcattc cggcctttct cagcctcaaa
      481 acatcagctg caatacgctg actaccagac cagaacaggc cgtccagtgc agtcaccagt
      541 aaccccgcct ccagcgcata cttcagccgt tcacggggcg ctttcacttc ccgggcaacc
      601 tgctgatatg gcaatgatgt gttttcactc ccgaccatcc ggcgaatacg atgagacaga
      661 tgataccggt atgtatccgg cacacctgaa aggctggcct tcaggctgta cacgcagcca
      721 aagtgtttat cgttgaacac cacatttttc tggctgatat cccattcttc acgcagcgcg
      781 gcaatcagtt gtggtgtacg ggtaagcaac aagcgaaaag gcagttcaaa actggtgacg
      841 taatccacat tcagcagggc aatgcgaagt cgttcttctg gtccggcttc tgtctgccgg
      901 cactcctcca ggacatcctg ccactgcagg cgaagacggg aagactcatt cagttctgta
      961 aagcagtatt tatccgccag atagtcaatt cgtgtatgca tactgaagag tattccgtat
     1021 aaagattcag ctggcaaaac tttatcagtc tgtaaaaact aacggaagag tcgatatttc
     1081 tcccgtccat caccggatga ttgttgcaat accccgtggc atcagagact gaacagccgt
     1141 ttttaacgca acgtattgct ctgatgtatc aggccggaca acccgaaaac acctttccac
     1201 ccggcattgt ccgccagcgc ttatcaccgg ccaggtctgt tgcagtaaat ccgccacttg
     1261 cgaacatgct tcatcaactg tgacactggc ccgcggatgg caaacactcg tctggctgag
     1321 cagcaacaga catcgtatcg gtgctccttc atgttgttcc cgcaaccagc gtaataccac
     1381 cggcgctgat gggctgaccg tgtgatcacg ctccgtaata cgttcgtgca cccgtcggtg
     1441 aaaggaacta cagaatgtct gaatctgttg accacagacg ccttctgtcg aatgaagtgt
     1501 gaagtggatt gccagcagat gcggccagtg atccaccgcc tgctgaacaa aacgccggat
     1561 ttcccccggc tctgaaagta aggcttcggt tatttgcact attttatctc tgttgaactt
     1621 ggttaagtcg gtgcagacgc atcaacacaa gtacggttcg atgcaaacag ctgtgactgg
     1681 caatatgaaa ggaatgatga atcagtcagg atgacaaagt gccagctgac cggaggggac
     1741 gcaggaagat tcacgggggg accagcacca gggaacagcg ccacaatacc aacgctgaca
     1801 cgttgaacat tgccagcgta ccggtatcac aacacgtttc atacttctgc caccgtgatt
     1861 cttcgattcg ttactgtatc tactgtgaca cttcgctttt atacctgcgg ctggatcggc
     1921 ccggcttgat gaatcttcac tgatcagctt ataaacccct ctgtcggtca taccggtgaa
     1981 actggtgata tagttcatgt caatcaggga attatcggca cgcagaaata cgctgtcgtg
     2041 gcttgttgta gtcaacatgg tcagaatgtc ctctgtgaga tttatgaaga ttgtgcgaat
     2101 gcggggaatc tgctgagctg tgctttcaga actggcctgt tacgggatac cagggattac
     2161 cggcggggta acgggcttcc ggatcataca caccacgatt atcgcggaca aaatcactga
     2221 acgcccatat cacctcttta agtatgtctt cgcagtccgg tacatgacga tccagcgcca
     2281 catcccgggt ggtactactt tgatgcgccc ggtgacacaa agcccggatt gttccagaca
     2341 tcctgaatca aacgccccag attaggggcg tcgaaatatg cctctctgac cattatattc
     2401 cggcgtacag gtagcaggtc agaagtgaca atgcgtcacc tgacgttaaa agtcactaca
     2461 cccaagatga cgttcaacag caccatgcga ttcaatgtaa gcccgggctg tctgttccag
     2521 tacaccaggc tcagcgttgt atgtgttagc tgcatcaaat accaacgaca gcacttcagg
     2581 atacacaacc agatgtgtaa tggagttatc ttcacccaat acttttcccc acgcctgctc
     2641 aatcagattt ctgagaacca ccacctcacg actcttacac cagacatcgt tattaagtag
     2701 cagcaccata agataaggag tggtatcgtt agccacagcc tccctactcc agagataata
     2761 taaaggggtg ggctcaacag atttatcttt acgtcgctta cactgcaaat attcagaaat
     2821 gagtctatgc agttcaccag taaaatcagc catcaaagag ggaatggcct tattaatacc
     2881 ctgccctggt attaatttaa attgtaataa tttaatttca ggatgtgtgg ctgcagcccg
     2941 atacagagtt gcaaggacac acttttgcca gagggcgtta caggaaagct taacgtttga
     3001 ttctgtatac ataataaatc accttacagt tacaacaggt caaaaaccgc tgtagccaga
     3061 gttacgctgg cctgatgctt tagtaccggg cttcgtcaga taatccagac gctccaataa
     3121 gcgctgatac tgctcaggga aatcaggatc atgaatatcc aggatgtaac gtccattagc
     3181 agggaaatga ataatgcagc cccctggatt aacaatgcag aaatcgtcct gaggcactga
     3241 tcaatacgga gaggactctc gcgtgtggtt tattgacacc acagtgcaga ttcggcgaat
     3301 ccgcgatcac ggtgcaattt cgttccacag cacacaatca taaccccggg ttttattcag
     3361 gtaagcagga ctgcggatat ccggtgtcgc gcctttgtgt cacgaacggg gtaggtgcga
     3421 aacaccggat aaaatgcagg ctggcaatac ctctgaacgc cctgcgcaga gcggatattt
     3481 tggattaagt actcgcacct ccgcagtcct ggcacaaatc tggctggtac ctgtaaacag
     3541 acttcgtaca tgttgctctg caataaatcc ccgtaccaca ggcctcacag aatttcttcc
     3601 catgaaaaaa ctgctcacac atcacacaat gccactccag cacgaccggt aatggcgata
     3661 gaaacatcgc catatcctca atgtaagggt gggacttttc cggattcagc accacgcagg
     3721 tcgccttctg ttgcgcgctc agggcatgtg aatcatgctc aaaccacgcc ccctgagcat
     3781 ctgtctgcaa aatcaaccga ccacgacagg aaaggcagaa acactgccgg atatttctgc
     3841 tgagcgcaga gtcggcactg ataatgtgtt cacccggcgt gatccccagc cccgttttta
     3901 taccgttcat tcagccactc cctcctcact gaagtgccct gtatggcagt gtgtgcagta
     3961 ccgctcccca taataatcgt ggtgacattg tctgcagcgc cagctggctt tacgcaccac
     4021 gggtaaggca tccgggacga attgctgcag acgtttaatc aaccgtattt ctctgcgttc
     4081 cggcctgaca taaggacatt gctgcgcgtg ctccgtcagc ccttcgtctg cgtgttcaaa
     4141 ccagggacgc tcagtgtcgt attgcgggtg gtatctgagc gcactgccgc aaaggtggca
     4201 agtgtagcga tcgtaaggtg cagtctgtgc ggtacgggca cccgtcagac gcccgttgcc
     4261 atcaaatgca ataaaggatt ttgcgtacat cgtcatattc ctcagccgcc agacgacacg
     4321 caggcaacag ctgtcccggt acgggcagtg tggtcagggt gtgaatggcg gttcagttaa
     4381 aatctggact ggagtttggg taaaacaggc tggttgctgt taccgcactg tggttgtcac
     4441 acaatggccc acctgcgtaa aaaactgtca gcaggcaggg atggacatcg ggggatgtgc
     4501 tgtatcggtt gccggaagga atggtaaaga atgtgccatg gttaaatctc cgtatggttc
     4561 atacggaata gccatgtcgt aaaaaatgcg cagagtccct gaagtggcct ccggaaccga
     4621 ctgtaacaat tagcgccagc ccgtagttgt cgttcagggg cttacaccag agaacatatc
     4681 tccgacaggg tactgtccag tcgcggtcgg ccccggttac ttttttcggg aagcctgcta
     4741 cgccccagaa taataccgtg ttctttctga tcctgtttag ccaggtagct gagggcgtaa
     4801 cgtaagtcgt ctaccgcaga tttatcacta tagtgaatca cgtgatcgat acgcaccggg
     4861 tatttgtctt tagcccggca cagatgaaaa taaccctccc cttccgtgat ccgcctccag
     4921 atatctccca gttgcctgga tatctgataa gagttcttgt gacgttgtcc atccagatag
     4981 ccgataaaat gaatatgaag ccctttgtct ggtgtatatt ccattaccca gtaatatcct
     5041 gctaacatcg tctgggtttc gctaagtaaa cggtatattt ccatacacat actgtgctta
     5101 caggaatgcc cgaaactggg cgtgtctttc ctgtaggcaa aatcaattct gaaaggtaac
     5161 agtttagaaa aacgttgaaa cataccgtcc atgtgttcat tcacatcttt cagaatcgcg
     5221 aaattcattt cgtaattggg attggcatta tacattttat agtccttact ttataaaact
     5281 cacaggaaga ggaaattatg taaatgctga gggtacagaa acgtcgtact acggcattaa
     5341 taaaagagat aaccagcagc acagacaacc tgataaaact gttttgtatt acctcattac
     5401 agcaatgagg taatatttca gaatacagtg agactctctt aaactgagga ccagtacaaa
     5461 ttacaatggt aaaaacacca cctcatagaa ttgagtttaa gaaaacacca ttcactcaac
     5521 ctgacattca caaaagtcag taccaggtag cctcaggtaa catcgctatt aatattatcc
     5581 tgccattaag taaaaacaga aactctgtga ggaagcctca ccaatatttg accagtagca
     5641 taaagaataa cgatatgaga gatacgccca caccagagat gattagccca tacaaaaaga
     5701 taatatttat taataccacc gaacctcaat agagtaataa cacactaata gcgttttagt
     5761 acacacttaa cactaactat gcaccagaca catactcagg attaacattc tcaacattta
     5821 tggtactctc aaagagtata attcaccaca caggtaaact ctcacaccag tagtatggag
     5881 ataatgctgt acacatactt acagagtaaa gtgtacagct agcatacagt ggcgagcaga
     5941 cattacaaca taaagactca agatagcata ataaagcata ataagaacac aacaaaactc
     6001 ccactaataa tactaattaa ccttaattct tttttgaaca ggaaatcaac atagctccag
     6061 acctcttcag gtcatacact tttgtattgg atgttcaatg tgtacttctg cacagacaaa
     6121 aaaataaaac ccacaaaata aaatatcaat cccaataaaa aattttaaaa tacagcgtga
     6181 aacaccacgt tatggatatg ttttcatatc accagaagca tatttcacac atacaataca
     6241 ggaacaattc atcatataat cagggttact gttcactcct cacggttatc cgcgagacga
     6301 taaaacatga cactaatcac aaaaacgaga gatagtattt gcaaaatgca aacaagataa
     6361 aaatcaatgt gttgcgattt tttatttttt cagacatgca tgccccctct gctattgttg
     6421 ctagccgact agcaacaata gcagggatat acaaacatcg gatgccaaaa acacgttccg
     6481 ataatatcat ctagggtctg actggttgtt tttagtatca tcaccacaaa tgatggttaa
     6541 tgcgttgtag atcagtctgc gtctgttctt gttttcagca ttatttccat acaacatcat
     6601 gacgttttta atatcatcag ccggtgatat tacataatca accaatacgc caagcttctc
     6661 aatacattgc agtaaagtat atttgtgtct gcccgtaaat aatcctatca ggttttcttt
     6721 actttcacga ctggataata ttttgttctc tgtaattaat tcagccaacg tttcaccatc
     6781 gtaaacacgt tctttatcta tcagaatatt tatcttatct aacgccctct ctacattatc
     6841 gagaagacct gtaatatacg gaggctgaat atctgacttt gtactaatat ttgcaagcat
     6901 cgaattatac attttaatga cacgcatcag agtaaaatta t
//
LOCUS       DAZGAP010000228         6793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGAP010000228 DAZGAP010000000
VERSION     DAZGAP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.229"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(121..723)
                     /gene="fimB"
                     /locus_tag="ACHLAM_004603"
     CDS             complement(121..723)
                     /gene="fimB"
                     /locus_tag="ACHLAM_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO7856030.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2175..2891
                     /gene="nanC"
                     /locus_tag="ACHLAM_004604"
     CDS             2175..2891
                     /gene="nanC"
                     /locus_tag="ACHLAM_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO7856031.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2911..4017
                     /gene="nanM"
                     /locus_tag="ACHLAM_004605"
     CDS             2911..4017
                     /gene="nanM"
                     /locus_tag="ACHLAM_004605"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO7856032.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRVYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4082..5062
                     /gene="nanS"
                     /locus_tag="ACHLAM_004606"
     CDS             4082..5062
                     /gene="nanS"
                     /locus_tag="ACHLAM_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7856033.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYSSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(5070..5720)
                     /locus_tag="ACHLAM_004607"
     CDS             complement(5070..5720)
                     /locus_tag="ACHLAM_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7856034.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            5857..6000
                     /locus_tag="ACHLAM_004608"
                     /pseudo
     CDS             5857..6000
                     /locus_tag="ACHLAM_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(6708..6791)
                     /locus_tag="ACHLAM_004609"
                     /pseudo
     CDS             complement(6708..6791)
                     /locus_tag="ACHLAM_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025205684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         2019 a         1437 c         1481 g         1856 t
ORIGIN      
        1 tttattatga taattaagaa tcacacttac aataacatca attaataaat ggttaatcat
       61 ttttgatata tcgtaagaat aatgtagttt ttaacaccat ccctggtatc tcaactatct
      121 ctataaaaca gcgtgacgct gtctccctct ggctctatcc cagatgccgt aaaaacgccc
      181 tgcattgctg gcggtatacc agacagtatg acgaatattg cgatgcccaa gataatcctg
      241 gataagtcgc gtatctattc ccatattcgc caaagcaaaa ccacacgaat ggcgtaacat
      301 gtgcggatga atctccagtg acaacccggc attaccaccg gaagtcgaga taatatggta
      361 aaactgttgc cgagaaagtg gattcccctt acgtgataag aatacccact cgctctcagc
      421 atgcggataa gaagtacgga tactcaacca gttttttaaa gcctgaattt ctttgttcaa
      481 tagcgggtgc gttgttgaaa agcctttttt taatcgatgg atatatatac actttgcctt
      541 aagatcaata tccgaaatcc tcaatcggca aatttcactc gcccggaaac catgaataaa
      601 acaaagcaaa gtcagacaat aattacgtgc tgcatgaggc ccggtatttg ctgctttaag
      661 gagtgattcg atttcactat gggtcaggaa gttccttttt ttgttatcag ccttattctt
      721 catcgttttc ccttataatt acagacgcgc actagctgtg ctgggttagc aataatccaa
      781 catttttatc ctgattatgt ttataaaagc gaacgtttgc ttaataacta acgtaagtgg
      841 accagttctt ctgagcgaac ttaaatggag tagcgatagt tacttataat aactttgcca
      901 tacaaacgcc atgctcttgc atgctatgta cctttatata tttatcaatc tgatacgaat
      961 cgaataataa ccctcatcat taatgcaact aatcttatct atatatcatg tgatatgttc
     1021 tgtaacaagt aaaatcaaca taaaaaattc agcaaaattt aatattcgtt caactagccg
     1081 tctcacatat gacattatta gtcaactgca tttgccacaa aatggctatt tcatggaacg
     1141 cagtaatttc ttagtggcta ttatcatgct aattcttaga tccctagcga ttattcctgc
     1201 ctggttcgtc agcttaggcc atgagattgt tacattccct tttatcctgg agatatgggg
     1261 gaacgcaggc gacgcttttt catctacatc cgttggtaac tatggctttg gtcactacga
     1321 cttaaacctt cctcaatcac agctattcgc cgggcgatac ggcaaacaaa ctaagtgaaa
     1381 catcatcctg aaaacaccaa catcaacaag cctctccaga tcgacatcag gagtgaccag
     1441 ttacaagcca caaaccaaga aacatatttc atgatgagaa ttatgctcag cctgatggcg
     1501 ggtaaacact tattttcacc aagctcactg agttagcttg caaagctttc tttgtttaga
     1561 ttctaaataa attttttata aaacagacag ttaaaataaa atctttaatt tttgaagacc
     1621 accgcaagtg ttcgtctggc ttcacatggc atctgcctct tagaaaaaga tcgacatatt
     1681 ttgtgacacg aattgcaaat ctcgttttgt tgtatggatt gcgtgatttt tgatctggta
     1741 taacaggtat aaaggtgcac cgcgatagtc aatgagacag ggcatctcgc aatctatggc
     1801 aaacatcact tcagttcttt ctcatcgggt gatgaaaacg cacttcagtc tgaaaggaat
     1861 atgaaaatga gatcaacaga cactctattt tatgactctg ggtaaaatgg attgagtaag
     1921 tgatatagct tacgaacatt caaatcaatt aaacatcaga agagatttta tactcaggta
     1981 tttaatctgg ttctctgttt atttaaataa tgtggaaaga gaattttcac aggagacctt
     2041 atacaaaaaa tataaaataa gctacccgtt gccaaagaca ctataagcct ggcaaaaaat
     2101 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
     2161 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
     2221 cgtcaccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagcggaa
     2281 gccatgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
     2341 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
     2401 atgatgttca ggttgaagtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc
     2461 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
     2521 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
     2581 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
     2641 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
     2701 aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc
     2761 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgtc
     2821 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
     2881 ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc gcttactatc
     2941 ataatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
     3001 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg
     3061 gcatggtaca agctggatac acaggccaaa gataaaaaat ggacagcgtt agctgcattc
     3121 cctggcggac cgagagatca agcaacctct gcatttattg atggcaatct gtatgtgttt
     3181 ggcggcattg gcaaaaacag cgagggcttg actcaagtat ttaatgacgt acacaaatac
     3241 aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggc
     3301 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
     3361 ttcaatggct attttgaaga tctcaacgaa gctggaaaag attcaaccgc tatagataaa
     3421 atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     3481 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatctccctg gtacggaacg
     3541 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     3601 ccaggattgc gaacggatgc cgtatttgaa cttgacttca ccggtgataa tttaaaatgg
     3661 aataagcttg ctcccgtcgc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
     3721 aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcgcggga aaattaccaa
     3781 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
     3841 tggcataacg ggaaatggga taaatctggt gaattatcgc aaggtcgggt ctacggagta
     3901 tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg
     3961 gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc
     4021 ttcagggcct cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta
     4081 tatgaacgca ataatatcgc ccgattatta ctatgttctt accgttgctg gtcagtctaa
     4141 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcatcccag
     4201 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa
     4261 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     4321 tcctctggca acaaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
     4381 acggaaatta ctgcccttta ttcctgataa cgcaggggtt ctcatcgttc cgtgttgccg
     4441 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     4501 ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac
     4561 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg
     4621 cgaatttgac ttaatgacct gtgactactc gtcacaccct caacacttta atcatatggt
     4681 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     4741 accgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga
     4801 agcgatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     4861 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gatccggacg atttaagcac
     4921 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     4981 tcatttcagc gcggcagccc gtcgggggat tatttctgac aggtttgtag aagcaatttt
     5041 gcggttttgg cgcgaaaggt gagcgttcat taatttgatt tatttgcatc catcaacgct
     5101 tttaactcct ccggtgaaaa cggtggtttg cgtcggtgca gcatcgcatg gcaattaggg
     5161 cacacaggca gcaaatcatt aactggatcc agctggtaat cctctttaat ggctgaaagc
     5221 ggcaccaggt gatgcatatg gataaagcct tcagcgatat caccatagat cttcaccaga
     5281 tcaacgccgc atacgttgca ctggcatcca tgatgttcga ccgctttatc tcgggccttt
     5341 ttatcgcgtt catagcggtt tactgtcacc tttatcgccg caccttcaag gtatgtctcc
     5401 tgcgacggca actcatccgg gaacagaacg tcggctatgg gagccgcagc ggctttatcc
     5461 tgatgaatat gaaatccctg ctctatgagt agtttgacgc atttagattt tattccgccg
     5521 gtgaaatccg caggggtaaa ttgcgtcccc gtcatcaatg tcgctgcgat gccagcaatc
     5581 gccttcgagg ggtaacgtcg gccatgatat tcaatctcat acaaccgcgc aggctggaac
     5641 tgatgagttt ctcccgcatc ccataaatgg atagcttcga tgatataccg ccgcggaaga
     5701 tgatcaggga gtttattcat ggagaatgtc cttatacgtt ctcagaacag gcagcaaaaa
     5761 aacaaatgag atcaaggtaa tatatcttca cttgcaggct tgaacagcag aaaatttgcg
     5821 agcatgatcg caattatttc actgccaggg atatcaatgg cttccagtaa atcgctacag
     5881 caggcaattg ccaatataaa aatctggcac aagggtgaac agcgcgcgcc gcataagcca
     5941 ttgttattgc tatacgtatt agcgggatac ctgaatggac atccgcgcct tttcgattat
     6001 tgtagtggaa cagtctacga ggatgcagtt cgaagtgagt aagtaaaggt tgctggaaac
     6061 gatcaatgcc gccatacaat gatttttgag gatcaacaat gagtaataaa aactacgaaa
     6121 gtcaccgcaa agcgattgtt agtaaaggta taccacccgc tctattaaat aggctcacca
     6181 attcggatgt tcaggtgatc aataccttct taacacgagt gagcaaactg gaactatctc
     6241 aacaagagaa agactggatc ataaaaatca tctctatggt ttagacaatt tcagccttgt
     6301 agcctttcaa gggttttgtg tgggtacaaa cattatcgaa tcgggatata gccgcagctg
     6361 tagctgtatc cacatcagta agttgatgta ggttgactta ggttgagcga aaaatagcga
     6421 aaagccttgt gtggcgcgga tttcagtcat aaaaaaacgt ccgttgacgt ctattgatgt
     6481 tccgatggtg cgaaggtcga aggccggact cgcctttgcg tttaattcgt tgaaaaataa
     6541 tggtttgtga gattttattt tgaggatttg cccccaaaat gggacataaa attgaatact
     6601 gggtggaaga tcacaaagtt gcgtaattat tttccaggtc cgaaacgcat tcaagagcaa
     6661 ggcctctccc ggcactgcgt cagtcgggat gctaaatctc ccacatgtag ccctagtaaa
     6721 tcgcgccaga atgtcaggct agtttgcaaa ttactgacgg cgagcgttaa gtggttcaga
     6781 gattgaagca tag
//
LOCUS       DAZGAP010000229         6722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGAP010000229 DAZGAP010000000
VERSION     DAZGAP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.230"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..257)
                     /locus_tag="ACHLAM_004610"
     CDS             complement(<1..257)
                     /locus_tag="ACHLAM_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HFO7856035.1"
                     /translation="MRSLFALLITVISCTTASAAMTETCQKYFDETDKFLSQMKTTGV
                     AEGQINLMKQQYEQSKKQFSSLPEASQDAACKQASDALKQS"
     gene            complement(1011..1259)
                     /locus_tag="ACHLAM_004611"
     CDS             complement(1011..1259)
                     /locus_tag="ACHLAM_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7856036.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESVQEFVQETLQEVWETADDWFIH"
     gene            complement(1485..1580)
                     /locus_tag="ACHLAM_004612"
                     /pseudo
     CDS             complement(1485..1580)
                     /locus_tag="ACHLAM_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1577..2143)
                     /gene="yecD"
                     /locus_tag="ACHLAM_004613"
     CDS             complement(1577..2143)
                     /gene="yecD"
                     /locus_tag="ACHLAM_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7856037.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2453..4225
                     /gene="aspS"
                     /locus_tag="ACHLAM_004614"
     CDS             2453..4225
                     /gene="aspS"
                     /locus_tag="ACHLAM_004614"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO7856038.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKDENN"
     gene            4343..4795
                     /gene="nudB"
                     /locus_tag="ACHLAM_004615"
     CDS             4343..4795
                     /gene="nudB"
                     /locus_tag="ACHLAM_004615"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO7856039.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4824..5564
                     /gene="yebC"
                     /locus_tag="ACHLAM_004616"
     CDS             4824..5564
                     /gene="yebC"
                     /locus_tag="ACHLAM_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7856040.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5599..6120
                     /gene="ruvC"
                     /locus_tag="ACHLAM_004617"
     CDS             5599..6120
                     /gene="ruvC"
                     /locus_tag="ACHLAM_004617"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO7856041.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6122..>6722)
                     /gene="yebB"
                     /locus_tag="ACHLAM_004618"
     CDS             complement(6122..>6722)
                     /gene="yebB"
                     /locus_tag="ACHLAM_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO7856042.1"
                     /translation="NVNYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGHN
                     GEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRK
                     LYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLG
                     SIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1715 a         1571 c         1729 g         1707 t
ORIGIN      
        1 attgattgct taagcgcgtc acttgcttgt ttacaggcag catcctggct tgcctcgggc
       61 agagatgaga attgtttctt tgattgttca tattgttgtt tcattaaatt aatctgtcct
      121 tctgctactc cggttgtttt catttgcgat aagaacttat ctgtttcatc gaaatacttc
      181 tgacatgttt ctgtcatagc cgcactggca gttgtgcagg atattacagt gattaataaa
      241 gcaaagagac ttctcatata tcctccattg ttttgtttga aaaattaacg ctattaaata
      301 actcatagac aggtaaattt atgtgattat agtcataaac tataaaaata ttgatgttgt
      361 ctttgtagaa aggttgtgag caattatgaa aatttatttg cttgttcact ggttttaggg
      421 agtttgatgt gtccatgaag cgtttctgac gagaaatcaa cataaatcac cagttggtga
      481 actaggattg ccagaaacgc tcctgtcagt tctgtagtgt gtagtgctca cttagagcct
      541 ggtgaactgg ataatgagtc gcacctcagt atcctgcaat gggctgcttt tcccctgctc
      601 ctgattcttt tttgtttcag gtcttaccaa cataatggtt gttgtaataa attgcgtgtg
      661 attctgtatc gggtcaacca tgtgacgata catgttcagt aaggaagact gaagggttca
      721 gagtgcgagt atgattaata gatggaatca gcgtttcaaa gattttgagg gaatataagg
      781 tctgacctga tgattacctt ttgcgcctgt atcctttgca taaaggaggt tgatgtagat
      841 aaagtcatct ccacttgtga tataaaacag tcagttatga aaaatcggca taaacatcct
      901 tttgttaacc ttaaagccag acttacggtt tttccatagt tacagataag cggctagtaa
      961 ggttttacag gtgataagcc tgtaacggaa atcacctgca aatctattaa ttagtgaata
     1021 aaccagtcat ccgctgtttc ccagacttcc tgaagcgttt cttgtacaaa ctcttgcacg
     1081 gattctttat ccacggctct taaaatagtg agtccatcat tgctggcaga tttaacgata
     1141 acatctacat cgtcataacg cttaccgaca cgtcgtagca tttcctgttg cagggcagga
     1201 acagaacctt ttggcatttt gctgattttt tctttagcga tacagatctc aacacgcata
     1261 acaccctcct gtaactgtgg ttatgtacag gtgttatttt tatctgtatg gataaccagt
     1321 gtcaattcct gatgttgttt atggggcatc aatggggcat gtatgggaca tttttaatcg
     1381 gcgaaatacg tcgaagttcg tcgatatggt aaacgaatca tctatccaac ccttgaaaaa
     1441 cggcgctcct ggacgatctt cgtcgatttt taaaaatgtt gcgtcacgca gttaaagtgg
     1501 cgggcatact cttcaaggtt ggtgatcccc aaccgcaccc atttaggatg cgaccattgc
     1561 ggcagaccga tgtaaatcat aacgcgtgga ggatctcttc cacactgcgc acacgggcga
     1621 tgcgcgggta gatatgatta atgctgttat tgtgctgctc ggcgctggcg gcgctacagg
     1681 catcttcggc aatcaccaga ttaaaaccga gttcccaggc attgcgggcg gtggattcaa
     1741 caccgatatt ggtcgagatc ccacataaca ctattgtatc gataccccgg cggcgtaatt
     1801 gcaactccag atccgtaccg taaaacgcac cccattgacg tttgatgatt tcgatatcgc
     1861 tgtcggttgc acctaatgca gcaggatgtt gccaccagtt ttcgggcaac acttttgcgg
     1921 gggagggggc atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaacgc
     1981 gcaccagaaa cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat
     2041 taaccacctc atcggcagta tgtggacctc cggcaaaagg taagatgcct tcttgtaaat
     2101 caatcaccac cagcgcggtg gttttagcat taagttcaag catgatgact cctgacaaaa
     2161 caaaatcgtg cctctcacct taatgcgatt agccgatggc ggaacgacaa attttgttaa
     2221 tttttatgag ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc
     2281 gatataaagt ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt
     2341 tccagtataa tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg
     2401 gtcacctgcg cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac
     2461 agaatattgt ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg
     2521 ggtcaaccgt cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg
     2581 tatcgtgcag gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact
     2641 gcgtaatgag ttctgcattc aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat
     2701 taaccgcgat atggcgactg gcgaaatcga agtactggcg tcctcgctga ctatcatcaa
     2761 tcgtgcagat gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa
     2821 ataccgctac ctcgacctgc gtcgcccgga aatggctcag cgcctgaaaa cccgcgctaa
     2881 aatcaccagc ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc
     2941 gatgctgacc aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca
     3001 caaaggtaaa ttctacgcgc tgccgcaatc cccgcagttg ttcaaacagc tactgatgat
     3061 gtccggcttt gaccgctact atcagatcgt taaatgcttc cgtgacgaag acctgcgtgc
     3121 tgaccgtcag cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca
     3181 agtgcgtgaa gtgatggaag cgctggtgcg tcatctgtgg ctggaagtga agggcgtgga
     3241 tctgggcgat ttcccggtaa tgacctttgc agaagcagaa cgccgttatg gttctgataa
     3301 accggatctg cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga
     3361 gtttgcagta tttgcaggtc cggcgaacga tccgaaaggc cgtgttgccg ctctgcgtgt
     3421 tccgggcggc gcatcgttga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat
     3481 ctacggcgcg aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg
     3541 tatcaacagc ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg
     3601 tactgccgcg caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc
     3661 cgacgcgatg ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag
     3721 caaatgggca ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg
     3781 cctgacggca atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa
     3841 agctgcacca gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt
     3901 gggcggtggt tctgtgcgta tccataatgg tgatatgcag cagacggtgt ttggtattct
     3961 gggtatcaac gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata
     4021 cggtactccg ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac
     4081 cggcaccgac aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct
     4141 gatgactgaa gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt
     4201 tgtgaagaag gacgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat
     4261 cgaggcggca actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga
     4321 aggcagtcaa cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt
     4381 agtggtcatc tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc
     4441 cgatttctgg cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc
     4501 catgcgcgaa gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat
     4561 tgactgtcag cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc
     4621 gggcgtgacg cgtaatacgg aatcatggtt ctgtcttgcg ctcccgcacg agcgccagat
     4681 cgttttcact gaacatctgg cttacaagtg gcttgacgcg cctgctgctg cggcgctcac
     4741 taagtcctgg agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg
     4801 cagggctatc tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc
     4861 gtaaagctgc gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg
     4921 taaccgcggc taagctgggc ggtggtgatc cggacgctaa cccgcgtctg cgtgcagcaa
     4981 ttgataaagc actgtctaac aacatgaccc gtgacactct gaaccgcgca attgcacgag
     5041 gtgtgggcgg tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg
     5101 gcggcacggc aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag
     5161 tgcgccatgc attcagtaaa tgtggcggta acctcggtac tgatggttcc gtagcttatc
     5221 tgttcagcaa aaaaggcgtg atctccttcg agaaaggcga tgaagacacc atcatggaag
     5281 cagccctgga agccggtgct gaagacgttg tgacctatga tgacggcgcg attgatgtct
     5341 acactgcatg ggaagaaatg ggcaaagtac gcgacgctct ggaagcggca ggtctgaaag
     5401 cagacagcgc ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg
     5461 caccgaaact gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt
     5521 accataacgg tgaaatctct gatgaggttg cagcgactct ctgatgaggc ctgctaaaca
     5581 gcaaaacgga gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc
     5641 ggctatggcg tcatccgcca ggtaggcagg caactgtcct acctgggtag cggatgcata
     5701 cgcaccaaag tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa
     5761 atcatcaccc agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac
     5821 gctgactcag ccctgaaact gggtcaggcg cgcggcgtgg cgattgtggc agcggtgaat
     5881 caggaattgc cggtatttga atatgcggca cgtcaggtaa agcaaacggt ggtaggtatt
     5941 ggtagtgccg aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct
     6001 aatccacagg cggatgctgc cgatgcatta gctatcgcga ttacccattg tcacgtcagt
     6061 cagaatgcga tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa
     6121 gttataccgc ctcggtcagt tccggctgag gcgtttccac tccctccgca tgaatcaaca
     6181 ccaaacccgg atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag
     6241 aacctaagaa ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacaatt
     6301 ctccaaacgt ctctatttca cccaccggaa tacatagcgc ttctttataa atatcaaaaa
     6361 caaattttga acagaactgg cgcgaagatt cgtatttaaa accggtgtgg taaagtttgc
     6421 gtagccggga gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt
     6481 ctaatcgctt tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg
     6541 tgatggttga aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga
     6601 taatgatccc gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg
     6661 tagcacctat acatgtaaag acgatatcac caatttcata ttcagcagga taattaacat
     6721 tc
//
LOCUS       DAZGAP010000230         6702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGAP010000230 DAZGAP010000000
VERSION     DAZGAP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.231"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..274)
                     /gene="yqfB"
                     /locus_tag="ACHLAM_004619"
     CDS             complement(<1..274)
                     /gene="yqfB"
                     /locus_tag="ACHLAM_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO7856043.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVIADIYPG"
     gene            319..1752
                     /gene="bglA"
                     /locus_tag="ACHLAM_004620"
     CDS             319..1752
                     /gene="bglA"
                     /locus_tag="ACHLAM_004620"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO7856044.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            complement(1916..4789)
                     /gene="gcvP"
                     /locus_tag="ACHLAM_004621"
     CDS             complement(1916..4789)
                     /gene="gcvP"
                     /locus_tag="ACHLAM_004621"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO7856045.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4908..5297)
                     /gene="gcvH"
                     /locus_tag="ACHLAM_004622"
     CDS             complement(4908..5297)
                     /gene="gcvH"
                     /locus_tag="ACHLAM_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO7856046.1"
                     /translation="MSNVPAELKYSKEHEWLRKEAGGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(5321..6415)
                     /gene="gcvT"
                     /locus_tag="ACHLAM_004623"
     CDS             complement(5321..6415)
                     /gene="gcvT"
                     /locus_tag="ACHLAM_004623"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO7856047.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT         1566 a         1796 c         1756 g         1584 t
ORIGIN      
        1 ggccgggata gatatcggca atgacctttt tcagatcagt cagggtcata ttttcctgct
       61 ctgcatgttt ttccgtcagt gtatccagcg ttacggttga ggttgcggtg acttcaatcg
      121 tgcaaaaata accgtcgtct tcaaaacgcc cgacacgaag cacatcaccc gttttgaagt
      181 gcgattcaga ctcgtcgcgg atggtgatgg ttttacgccc agccagaatg tcatcctgga
      241 aacgttgaaa aaaagtgatg tcgtttggct gcatggtact atttcctgta aaaattgact
      301 catctggagc ctatgatagt gaaaaaactc accttaccga aagatttctt atggggcggc
      361 gcagttgccg ctcatcaggt cgaaggcggc tggaacaaag gcggcaaagg gccgagcatt
      421 tgtgacgttc tgaccggtgg cgcacacggc gtgccgcgcg aaatcaccaa agaagtgttg
      481 ccaggaaaat actatccgaa ccatgaagcc gttgattttt atggtcatta caaggaagac
      541 atcaagctat ttgccgaaat gggcttcaaa tgttttcgta catccatcgc ctggacgcgc
      601 atttttccaa aaggcgatga agctcagcca aacgaagaag ggctgaagtt ctacgatgat
      661 atgttcgatg aactgctgaa atacaacatc gaaccggtga tcaccctctc ccactttgaa
      721 atgccgctgc atctggtgca gcaatacggt agctggacca accgtaaagt ggttgatttc
      781 tttgtccgtt tcgcagaagt ggtatttgaa cgctataagc ataaagtcaa atactggatg
      841 accttcaacg aaattaacaa ccagcgtaac tggcgtgcac cgctgttcgg ttactgctgc
      901 tccggcgtgg tgtataccga gcatgaaaac ccggaagaga cgatgtacca ggtgctgcat
      961 caccagtttg tcgccagcgc cctggcggtg aaagccgcgc gtcgcattaa cccggaaatg
     1021 aaagtcggct gtatgctggc gatggtgccg ctttaccctt actcctgtaa tccggacgat
     1081 gtgatgttcg cccaggagtc gatgcgcgaa cgctacgtct ttaccgatgt gcaactgcgt
     1141 ggctattacc cgtcctatgt gttgcacgag tgggagcgtc gcggatttaa catcaaaatg
     1201 gaagacggcg atctcgatgt gctgcgcgaa ggcacctgcg attatctcgg tttcagctat
     1261 tacatgacca acgcggtgaa ggccgaaggc ggcaccggcg atgcgatctc tggttttgaa
     1321 ggcagcgtac caaacccgta tgttaaagcg tctgactggg gctggcagat tgatcctgtg
     1381 ggtctgcgct atgcactttg cgaactgtat gagcgttatc agaagccgct gtttattgtc
     1441 gaaaacggtt ttggcgctta tgacaaagtg gaagaagatg gcagcatcaa cgatgactac
     1501 cgcattgact acctgcgcgc ccatattgaa gagatgaaaa aagcggtgac ttacgatggc
     1561 gtggatctga tgggctacac accgtggggc tgcatcgatt gcgtgtcgtt cactaccggg
     1621 cagtacagca aacgctacgg ctttatctat gtgaataaac atgacgacgg tactggcgat
     1681 atgtcgcgtt cacgtaagaa gagctttaac tggtacaaag aagtgattgc cagcaacggc
     1741 gagaagcttt aattacccga tgtgcctgat gcgccccgct catcaggcct ggcgctcctg
     1801 tcatatattg aactccagga gtttatattt acgcttcatc cgacacacaa agcgcacttt
     1861 attaacaagc taaaaaggcg ccgaagcgcc tttagaagat agtcgaatca gtgaattact
     1921 ggtattcgct aatcggtacg caggagcaga acaggttacg gtcgccgtaa acatcatcca
     1981 gacgtttcac tgtcggccag tatttgtctg ccacgcctgc cgggaatacc gcaacttcac
     2041 ggctgtacgg atgcgcccac tcggcgacca gttcgctctg aatgtgcggc gcgttcacca
     2101 gcgggttatc ttccagcggc cagacaccgg ctttcacctg gtcaatttct gcgcggatag
     2161 ccagcatcgc gtcgataaag cgatccagtt ccactttgct ttcagattca gtcggttcaa
     2221 ccatcagcgt acccgccacc gggaacgaca tggtcggcgc gtggaaaccg tagtcgatca
     2281 gacgcttggc aatatccagt tcgctgatgc cggtttcttc tttcagcggg cgaatatcga
     2341 gaatacattc gtgcgccacg cgaccgtcgc gaccggtata cagcaccggg aaggcatcct
     2401 gcaggcggct ggcaatatag ttggcgttaa gaattgccac ctggcttgct tttttcagcc
     2461 cttctgcgcc catcatgcgg atgtacatcc agctgattgg cagaatggat gcgctaccga
     2521 acggtgccgc agaaaccgcg ccctgacggg ttaacatgcc ttcaatttgt accacgctat
     2581 gacctggcac aaacggtgcc agatgcgctt tcacgccgat cggtcccata ccagggccgc
     2641 cgccaccgtg cggaatgcag aaagttttat gcaggttaag gtgcgagacg tccgcgccaa
     2701 taaagcccgg tgaggtgatg ccgacctggg cgttcatgtt cgcgccatca aggtaaacct
     2761 gaccgccgaa ctgatgcacg acttcacaca cttcacggat cgtttcttca tacacgccgt
     2821 gggtggaagg ataggtcacc atgatacagg agaggttatc gcccgcctgt tccgctttcg
     2881 cgcgcagatc agtcagatcg atgttgccgt ttttatcaca cgccacaacc accacctgca
     2941 ttcctgccat atgtgcagaa gcggggttag ttccgtgcgc agaagccggg atcaggcaga
     3001 tatcacgatg cccttcgttg cggctttcat gataatgacg aatcgccagc aggcccgcgt
     3061 attcgccctg tgcgccagag ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca
     3121 ccagccagtc agccagctgc gcaatcatct gctgataacc ttcggcctgc tccggcgggc
     3181 agaacgggtg cagttcggca aattccggcc aggtgattgg gatcatctcg gcggcggcgt
     3241 tcagtttcat ggtgcaggaa cccagcggga tcatcgcctg attcagcgcc agatctttac
     3301 gctccagcga gtgcatatag cgcatcattt cggtttcgct gtggtagcga ttaaacaccg
     3361 gatgggtgag gatttcgtcg tcgcgcagca tcgcaggctg aatagagcgg ctgtcgtgag
     3421 ccacgtcttt gtccagcgtg tcgatgtcca ggccgtggtt atcccccagc agtacgctga
     3481 aaagctgcat cacgttttca cgcgtggtgg tttcatcaag ggtgatccca accgcgttca
     3541 ggatatcgct acgcaggttg atttcagccg cttcggcacg cgccagtacg cccgccttgt
     3601 ctgccacttc cacacacaaa gtgtcgaaat agtgcgcatg acgcagtttc agaccttttt
     3661 gttgcaggcc cgccgccagg atatcggtca gacggtggat gcggttagcg atacgtttca
     3721 ggccaaccgg gccgtgataa acggcataca ggctggcgat gtttgccagc agtacctggg
     3781 aagtacaaat gttggagttc gctttctcac ggcgaatatg ttgctcgcga gtctgcatcg
     3841 ccatgcgcag agcggtattg ccagctgcat ctttcgatac accgataata cggcccggca
     3901 ttgagcgttt gtattcatct ttcgccgcaa agaatgctgc gtgtgggcca ccgtagccca
     3961 tcggcacacc gaagcgttgc gccgaaccaa agacaatatc cgcgccctgt ttacccggcg
     4021 cagttaacag caccagcgcc attatatcgg cggcgacact gaccacaatt ttgcgtgatt
     4081 tcagttcgct gatgagcgca gtgtagtcgt ggatttcacc ggttgtgcca acctgctgta
     4141 acagcacgcc gaagacgtcc tgatggtcga gcactttttg cgcgtcatcg acaatcactt
     4201 caaaaccaaa ggtttcagca cgagtacgga ccacatcgag cgtttgcgga tgaacgtcag
     4261 aagcaacgaa gaagcggttg gcatttttca gtttgctgac gcgtttcgcc atcgccattg
     4321 cttcggcggc agcagtggcc tcgtccagaa gagaggcaga ggccatatcc agcccggtca
     4381 aatccagcgt tacctgctgg aagttgagca gtgcttcaag gcggccctgg gagacttcag
     4441 gttgatacgg agtatacgcg gtataccagc ccggattttc cagcatgtta cgcagaataa
     4501 ccggcggtag ctgcacggcg gtgtaaccca tgccgatgta agacgtgaag cgtttattgc
     4561 gactggcaat agccttgagt tccgccagcg cggcgtattc ggtcgccggt gcgccaacct
     4621 gcggcggggt cgcaagctga atatctttcg gcacaatctg gccggtcagc gcgtttaacg
     4681 attgtgcgcc aacggcattc agcatttctt gctgttgcgc ggcgtccggt ccgatatggc
     4741 gttcaataaa agcgccgctg ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt
     4801 cctgaaacgt gcagtgaatt gtgaacctct ctccttgcga agagagtgag ggtgaggcgt
     4861 aaaagttcct caccctgatc ctctcccgca gaagaggaat aaagccgtta ctcgtcttct
     4921 aacaatgctt cgtatgcggt cgcatccagc agtgattcca gttcgctttc atcgctggct
     4981 ttgattttaa agatccagcc gcctgcatac ggttcgctgt tcaccagttc cggggaatcg
     5041 ctcagtgcgt cgtttaccgc cacgatttca ccgcttactg gcgcataaat gtctgacgcc
     5101 gcttttactg attcggcaac cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc
     5161 ggcaggtcaa caaacaccat atcgcctaac agctcctggg catgttcggt gataccaacg
     5221 gtgtaagtgc cgccggcttc tttacgcagc cattcgtgtt ctttgctgta tttcagttct
     5281 gctggtacgt tgctcatcaa tcaatctcca aaaaagtaaa tcacgcgacg gctttaccgt
     5341 tacgcacaaa aacaggtttc gtcactttaa ccggcatttc acggttgcga atttgtacaa
     5401 tcgctgtttc accaatacct tccggcacgc gcgccagcgc aatgctgtaa cccagcgtcg
     5461 gggagaaagt accgctggtg ataatgcctt catgctggtt gccctgcgca tcggtaaagc
     5521 gtaccggcag ttcattacgc agcacgcctt tttcggtcat caccagacca accagttttt
     5581 ctgtgccatg ctcacgctgc acttccaggg cttcacgacc gatgaagtca cgatctgccg
     5641 gttcccaggc gatagtccag cccatattgg cggctaaagg agagatggtt tcgtccatct
     5701 cctgactata aagattcata cccgcttcca gacgcagcgt gtcacgcgcg cccaggccgc
     5761 atggcttaac gcccgcttcc accagcgcgc gccagaaatc cgccgctttt tcattgggca
     5821 gcgcaatttc atagcccgct tcaccggtat aaccagtggt ggcaataaac agatcgcccg
     5881 cctgcacgcc aaagaacggt ttcatccctt ccaccgcctg acgctgggcg tcattaaaca
     5941 gtgtggcagc ttttgcctgc gcattcggcc cttgtacggc gatcatggaa aggtcatcac
     6001 gaacggtaat ttcgatgccg aaaggttcag cgtgttgggt aatccaggag aggtcttttt
     6061 cgcgggtggc ggagttaaca acgaggcgga agaaatcttc agtaaagtag tagacgatga
     6121 ggtcatctat cacaccgcca gaggcattca acatccccga gtaaagggct ttgccgcttt
     6181 tggtgagttt cgccacatcg ttcgccagca gataacgcag aaactcccga gtgcggctgc
     6241 cgcgaagatc gacgatggtc atatgtgaca catcaaacat tccggcatcg gtacgtaccg
     6301 catgatgttc gtcgatttgc gaaccgtaat gcagcggcat catccagccg tggaaatcca
     6361 ccatgcgagc gccgcaaagc gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt
     6421 cctcattgaa taagcggggc tgacaacttt tccatggcga aattatcacc acgaaaccca
     6481 gcatcggagc cactcccggt ccccaacgca atcgttctct tttgcctgaa cttaccaccg
     6541 aaacagactg ttaaccataa ggtaaaattg atcatcacat tagcttatgg ttaaaaaatg
     6601 caaaaatcgc aacagaataa aaaaccaaaa aacacatcag tttctataca aagatgatgt
     6661 gatgagaaag ttaatttgaa taagacaata ttaagcgcta aa
//
LOCUS       DAZGAP010000231         6678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGAP010000231 DAZGAP010000000
VERSION     DAZGAP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.232"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(264..2549)
                     /locus_tag="ACHLAM_004624"
     CDS             complement(264..2549)
                     /locus_tag="ACHLAM_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7856048.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAITGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQTHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIRITRTTDWRIVPKVPVVQPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKYGLRTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMARI"
     gene            complement(2539..2814)
                     /locus_tag="ACHLAM_004625"
     CDS             complement(2539..2814)
                     /locus_tag="ACHLAM_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO7856049.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2811..3035)
                     /locus_tag="ACHLAM_004626"
     CDS             complement(2811..3035)
                     /locus_tag="ACHLAM_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7856050.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(3035..3337)
                     /locus_tag="ACHLAM_004627"
     CDS             complement(3035..3337)
                     /locus_tag="ACHLAM_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO7856051.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3337..3561)
                     /locus_tag="ACHLAM_004628"
     CDS             complement(3337..3561)
                     /locus_tag="ACHLAM_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO7856052.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3625..4125)
                     /locus_tag="ACHLAM_004629"
     CDS             complement(3625..4125)
                     /locus_tag="ACHLAM_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO7856053.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4122..4292)
                     /locus_tag="ACHLAM_004630"
     CDS             complement(4122..4292)
                     /locus_tag="ACHLAM_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856054.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4303..4578)
                     /locus_tag="ACHLAM_004631"
     CDS             complement(4303..4578)
                     /locus_tag="ACHLAM_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFO7856055.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4700..4999
                     /locus_tag="ACHLAM_004632"
     CDS             4700..4999
                     /locus_tag="ACHLAM_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856056.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5115..6128
                     /locus_tag="ACHLAM_004633"
     CDS             5115..6128
                     /locus_tag="ACHLAM_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7856057.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6405..>6678)
                     /gene="yegR"
                     /locus_tag="ACHLAM_004634"
     CDS             complement(6405..>6678)
                     /gene="yegR"
                     /locus_tag="ACHLAM_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO7856058.1"
                     /translation="SGCAVHNDETSIGKFGLAYKSNIQRKLDNQYYTEAEASLARGRI
                     SGAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         1760 a         1706 c         1475 g         1737 t
ORIGIN      
        1 gaagcttcaa aactatcatt tacgtttgcg aggcaccatc tatcacattg atttataata
       61 agtatcttta tcttcactgc attgctctca aaaaatcaca tagatggaaa ttccccgttg
      121 acacactccc aagtgatgca gatacatttc aagtgcttca tacacatgat aacggctatc
      181 cccctaaatt ctgtgcgttt cttttccctc cagcacaagg ttgaaatatc ttgcgtgcag
      241 ctttactttg tgaatctttg tcattatatc cgcgccatta ctgttgagaa tcccggccac
      301 tcatcagcga ccgggtacgt gaattttttc ccgtcataat ttacggtcgc gccacgcgcc
      361 agcgcctcaa gctcccatcg ctgcggcctg ataccgttct gagcaaggtc aacgcggata
      421 cgggtaattt gcattctttc cgaccgggtc agtctggccg atggtgcaat ttcatgtggt
      481 tttaacgggc ttccgtttct ctgctgacga tttggggttc tcagcccgta ttttaatgcg
      541 cccctgagcg ccctcacgac ctccgggtca ttccattcga taacaccgtc atcaaccaga
      601 ttaagcactg ctgcggcgtg ttcagaaggt gtgggagccg gtaacgaagt atcaccaccg
      661 gtgagctttc cacagttatt gacaggactc cgaggcgcgg cgatgccgct ttttaaagtc
      721 aaaggctgaa cgaccggaac tttcggcaca atgcgccagt ccgtcgttct ggtgatacga
      781 atatgacgcg cgccgagatg cggcgcgtaa atgccgacca ctctctcgac ctcttcctcg
      841 tactcgttaa cgtcatccga ggggctacgg gcgaccctga cagtctggca atcgcgcggg
      901 acatttgccc caccctgcgc gctgatatac agcgcaaaat cgccactgtc tgcggcagcg
      961 cgtgcagcct cgacgcgttc gtcaaactca tcagcaatgc tgacgccgcg aggcaatttg
     1021 cgtagttcac ggtaagcccc cattgtcggc aggccaaccg ttttaaattg aggaatgcgc
     1081 cacgttgacg cccatgcggt aacagccgcg gcagtgtctt tcagcggcct gccggtgtcg
     1141 ttatcgagct gaccatccag tgcatagcca tcgatgtttt ttgaaatgta tttcgcgata
     1201 tatcccgcag caccgccccg gttaaggtgt tttgcctgaa aacggtttcg cgcggctcct
     1261 ctttcgtcgc catcctcttt gagcgcgtag cgacgcatga tttcgataat ctgattacgc
     1321 tggcgtggat tacaaaaaag catcatatgc cagtgcggcg ttccgtcgtg gtgtggctcg
     1381 acgacacgca aaccgtagac ctgtaaatca ttatccttga atgccgtgcg catcaggctc
     1441 cagatgcggc agagataacg ctgcgcatcc tttggattaa atgcctcatc gttccagccg
     1501 tgattaagct gcacggtttt actttcgcct tttccgacct gacgtgtcgg gtgatacttt
     1561 gacggtgcgg tcagcgtgat aaacatcccc acatcgccct ctgcggcggc gtaacgctca
     1621 ataccggcaa tggtgttcat cagctccatc cggcgaattt caggattaga aatactgccc
     1681 atcaccttac tgataaggtc gatgcgctcg ccggtttccc tgttttcgag gtcacacgat
     1741 ttaagaaatt ccagatttgc ctggcggcgc gcacgcacat cacgaatggc atgtttactg
     1801 gcataaggag aacggtcttt attgacctcc ccgacagcaa tcagtaacgc ctcatgccag
     1861 cgcatacgct ggcctttaag ctgactaatc caccactcat cgttaaacag acgggcaatg
     1921 gcagaatatg cctgcctcgt ggtcatctgc cctttacggt attttttcca gtagagcggg
     1981 gaaatattga aagcacgtgc agcgccagcg acatgaccat acaggtgagt ctgcgcctca
     2041 tccgtaaaca gcgattcttt ttcgccatgt gcatccaccc aggcatcgca gagttcctca
     2101 tacatcatga aaagctgcga tgagatacgg gcggcaaact ttttcagctc cttgtcattc
     2161 attccaggca ggcgcgcata atggtcacgc tctgccagaa acagcaacga cgcgtcggtg
     2221 ttcatttcat ggcgctgatt cacgcgctca atgcgcggcc ataaacgacg ctgaaaagtg
     2281 gatgtgagga aataaaaccc gtgcaccggg cttttattgc gccggatgta gtcataacgt
     2341 gaagtaaaca gcgagcgcaa aaagtaaggc aggcggttaa tcgtggataa aacaccttgc
     2401 acctgacgca tctcgtcacg tgtaaggggt ctttcgcgcc cgacagcctc gcgtggcgcg
     2461 ttccatgcat aagcaccggt aatcgcctta ccggtgcctg cggcaaatgc tgacggaggg
     2521 acaaaacgcc cggaggcttt aacggccata tgagccaaaa gcctcagaac aacgcttgct
     2581 gagttgctca acctgcgcgt ttaaatcagc aaaagacttt gcgcttccgg tcagaatatc
     2641 gtgatgcatc aggccggaaa cgagctggct taatttcggg taataaccaa ccaccgccag
     2701 ccattcctgc ccggcgtttt taccgctttc cgctctcttt ttctcgtgga gaataaactg
     2761 aaagctgtca ctggtaacga cataacgttc gccaatttcg atacgaatac tcatgccatt
     2821 ctccggtaat gcttgttttt tgcttcaaag actgactggc aggaaacaca acgcgtggct
     2881 gacggataag ctgcacgacg ggcagcaggt attggcgcgt cacactcttc gcaaaccagc
     2941 gcagaaacac cgcaatgttt tacccttgcc gcgttaatct ggcgctccag taattcagcc
     3001 tgttgttcct gaataaaatc tatgttgtcc ggcattacca gctccttttg tcgttcagct
     3061 tcttaaattc atcagcgcaa tagctggcga tttctgtcgt taattttgtc agttcatcca
     3121 cggatgaaat ttgcttgtgg aatacagcac gtttaacaag taaattgacc acatcagaca
     3181 ggaggtttaa ttcattctga taaatcgcga taacagattc agttatgtcg cgcttttctt
     3241 tatcaagaca aagttgaata agagacaaat caccattttc cataacggcg atttttaagg
     3301 cgttattcag taatacaact gaatgagaac aggacatcaa agtacctccc cgcgagacaa
     3361 tccgatattg tgaaattttt ccgactcctg actgagcagc tcgactatct ccacgcggga
     3421 taactccgcc tttgtgatgt ggcgaatcat ggcgtcaaga tgagaagaaa agcgcgtagc
     3481 tgcatctgcc tgtgcttcgg ttctggcctg ttgcagcagt aatgcgtatt taccgcactg
     3541 attttcagaa actgtatgca tgactttctc caggcaaaaa gaagccccgc acaattaagt
     3601 gcgttaaaaa ctctggttaa ttacttaatg cagatattgc tctggtttta ccgatgtcag
     3661 aattgtcggt gcatactcaa acagactgaa taattcacgt aatgcacgga ataaagcatc
     3721 acgccagtaa catgattctt cattaattcg ccagtacggc tggttgaatt ctttttctgt
     3781 cagtcgtgcg tgcataaata aagtacgacg ctggctgact gttaaaaaac taatatatgc
     3841 atactcactt gcaccaacct gacggcgttt tgagaatgcc ccacgcaatt catcaattgc
     3901 acataccagt cgttcacgtt cgacgtcgtt catttcttca aaacgcatcg ttgcgtgacg
     3961 ctgttttaac tgcgcatgaa agcaaaccgt tagccgttcg cgttccatca tctgattata
     4021 ataatcgcat gtctcctgcc agcgagggac ggccagatgc ttaccaatta tccggcgcat
     4081 agttgctggc tgtttttcaa cgagattgag cgtcatcact gtcatttcca taccctccgg
     4141 cttttcagaa aggtcagagc cttttttaac ggactctgtt ttttggtgcg gataatgatt
     4201 cccttacgcc ccttaccgtg ggtgatggtg aagtcaatcg ccctggggct ttcgttacgc
     4261 aataactgag caatacaacg aggctcattc atacggttct ccttaacgtg gttcaccgag
     4321 acctaaccac atcaaccagc cgtcacgaat ctctttagga cggctttcat aagccagttt
     4381 tagtccgtta ttccatgccg gaaggtatac ccaatattca ccagcacgcc ccgatactga
     4441 ctgagggtca gtaatctcaa taactggtaa tttccctttc tcaatcatac cccttacagc
     4501 tcttggagtt ttaccaatga gttttgcaaa ctcctgataa ggcacggcat cagtcacgct
     4561 tacaagctgt ctattcatct gctacgattc tcccttagtg cttctaatgg ctcctaatgg
     4621 ctaattattg cctaaaagga taactccaga agcacaacat ttcacactat cagcaagaaa
     4681 ttacgcaatc ggagtaatta tgtcaataga cgtttcggag aagttgaagc taatccgtga
     4741 atctgaaagg ttaaaccgta aagaattcag tgaattaact ggtgtagcct acagctcact
     4801 ttcgagctat gagagccggt caaaaaacgc tggagttgaa gccataatga aggtcttaca
     4861 acatcctaga tttactaaat atactttgtg gttcatgact gatcaggtag ctccagaagc
     4921 cgggcaaatt gcgcccgctc tcgcacactt tgggcaaaac gaaacaacgt cgccccactc
     4981 cggtcaaaag actggttaac aatttatcgt gaatatattc atcacaagtg cctactattg
     5041 gtggctaaat ttcagccacc acgaaaaaag cgattagtag tagcaaaaaa aagtaccact
     5101 cggagggttt tctgatggca atcaaaaaac tcgatgatgg tcgatatgaa gtggacatcc
     5161 gccctactgg acgtaacgga aaacgcatcc gtaggaagtt tgataagaaa agcgaagctg
     5221 tcgctttcga aaaatacacg ttgtacaacc accacaataa agaatggcta tcaaaaccaa
     5281 cagacaaacg acgtctgtcg gaactgacac agatctggtg ggatttaaag ggtaaacacg
     5341 aagagcatgg gaaatctaat cttggaaaaa ttgaaatctt cacaaaaata acgaatgacc
     5401 catgcgcatt tcaaatcacg aaatccctta tcagccagta ctgcgccacc cgaagaagtc
     5461 agggtattaa accttcgagt atcaatcgtg atttaacatg tattagcggc atgtttacag
     5521 ccctgattga agcggagtta ttctttggtg agcaccctat cagagggaca aagaggctta
     5581 aggaggaaaa accagaaaca ggctatctca cacaggaaga aattgcctta ctgcttgctg
     5641 ctcttgacgg cgacaacaaa aagattgcga ttctttgcct gagcactgga gcacgttggg
     5701 gagaagcagc tcgtttgaaa gcagaaaata tcatccataa ccgcgtcacg tttgttaaaa
     5761 cgaaaacaaa caaaccacgc accgtcccga tctcagaggc tgttgccaaa atgatcgcgg
     5821 ataacaaacg aggtttttta ttccctgatg ctgattaccc tcgcttcaga cgaacaatga
     5881 aagcaataaa accggatttg ccaatggggc aagccacaca tgcactaagg cacagctttg
     5941 ccactcattt catgattaat ggaggaagta ttatcacgct acaacggata ctaggtcaca
     6001 cgcggattga gcaaactatg gtttacgctc attttgcgcc agagtacctt caggacgcca
     6061 tttctcttaa tccgctaaga ggtggtactg aggccgagag tgtccacaca gtgtccacag
     6121 tagagtaacg tttaagggct ttcagtggta atttatgccg ctcaaacccg cattgtaccg
     6181 ttgaaagccc ctactggtga caccctaaat ctcccttaca cgggcttatt ttttatacat
     6241 aaaccataga gacggattga tcccaggtca ccgtcttcca ttgaccacat cgatagaatc
     6301 tcccttcata gcacgatgcc tttcacttat tggcatcgtg ctcgcacagg ttccggttac
     6361 gcacagccag aacgcgcatg tttgatgctt accaaaaaat attctcactc tccacatttg
     6421 aatgtcagac gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc aactatctgc
     6481 atttttttgc cctgagtaac acagaaatga actgcatcat tttttactat attttctgca
     6541 ccagatattc tacccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt
     6601 ttacgctgaa tattactttt atatgcaaga ccaaatttac cgatacttgt ctcatcatta
     6661 tgtacagcac acccagac
//
LOCUS       DAZGAP010000232         6650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGAP010000232 DAZGAP010000000
VERSION     DAZGAP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.233"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..630
                     /locus_tag="ACHLAM_004635"
     CDS             <1..630
                     /locus_tag="ACHLAM_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856059.1"
                     /translation="MQQPDKSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDA
                     PSGKDGAAFMAMMAEKARLAALLPEGWARDMTTFLSLSQDVLLSLLSFCTACSIHGVQ
                     TREYGHTSRSPLDSLESAIGFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARD
                     AEKMKKGDAAEHAEFHMKDNRWVPGWMCTPRPQAETETTEYRDDQAEAA"
     gene            699..1133
                     /gene="psiB"
                     /locus_tag="ACHLAM_004636"
     CDS             699..1133
                     /gene="psiB"
                     /locus_tag="ACHLAM_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7856060.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDEQGYSVSDIIHILMAEGGQA"
     gene            1130..1849
                     /locus_tag="ACHLAM_004637"
     CDS             1130..1849
                     /locus_tag="ACHLAM_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7856061.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSVLVMQKYSRQQAREAEQKARAYQTLVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            1846..2160
                     /locus_tag="ACHLAM_004638"
     CDS             1846..2160
                     /locus_tag="ACHLAM_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFO7856062.1"
                     /translation="MKWRYSLRWKLPSPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            2310..2462
                     /locus_tag="ACHLAM_004639"
                     /pseudo
     CDS             2310..2462
                     /locus_tag="ACHLAM_004639"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            2407..2529
                     /locus_tag="ACHLAM_004640"
     CDS             2407..2529
                     /locus_tag="ACHLAM_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7856063.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(2983..3156)
                     /locus_tag="ACHLAM_004641"
                     /pseudo
     CDS             complement(2983..3156)
                     /locus_tag="ACHLAM_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3456..3758
                     /locus_tag="ACHLAM_004642"
     CDS             3456..3758
                     /locus_tag="ACHLAM_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856064.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            3914..4201
                     /locus_tag="ACHLAM_004643"
     CDS             3914..4201
                     /locus_tag="ACHLAM_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856065.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
     gene            4319..5140
                     /locus_tag="ACHLAM_004644"
     CDS             4319..5140
                     /locus_tag="ACHLAM_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7856066.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRLEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRGITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(5438..6028)
                     /locus_tag="ACHLAM_004645"
     CDS             complement(5438..6028)
                     /locus_tag="ACHLAM_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7856067.1"
                     /translation="MVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFINVTC
                     KAGDCFDLAGRDYKIDPDLLRAISWKESRNRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASDVYRIYSGIKNSKGIQLRASEKPLSKMNSSQKN"
     gene            6363..>6650
                     /gene="traM"
                     /locus_tag="ACHLAM_004646"
     CDS             6363..>6650
                     /gene="traM"
                     /locus_tag="ACHLAM_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO7856068.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQKEFNKLLLECVVKTQSSVAKILGIESLSPHVSG"
BASE COUNT         1631 a         1605 c         1819 g         1595 t
ORIGIN      
        1 atgcagcaac cggacaaatc tctggcactg ctggcatgga cgctctgcct gaatgtgttt
       61 ggcagcggag cgtacagtaa accagcacaa atcagcctgg aatgtaaaca ttattcgctg
      121 accagcgatg cgccatcggg gaaggacggt gccgcattca tggcgatgat ggcagaaaaa
      181 gcccgtcttg ctgccctgtt accggaggga tgggcacggg acatgacgac gttcctgtca
      241 ctcagccagg acgtgctgtt atcactgctc agtttctgca ccgcgtgcag catccacggc
      301 gttcagaccc gcgagtatgg tcacacgtca cgcagtccgc tggactcgct ggagagcgcc
      361 atcggctttc acatgcgtga ctggtggcag ccgacaaaag caaacttctt cggtcacctg
      421 aaaaagccgc agattatcga tgccctgaat gatgccggac tgtccggtgc cgcacgggac
      481 gcggagaaga tgaagaaagg cgatgccgct gaacatgcag agttccacat gaaagacaac
      541 cgctgggtgc ctggctggat gtgtacacca cgtccacagg cagagacgga aaccacggag
      601 taccgtgacg accaggcaga agccgcctga ccgccagtca taaaccattt atcacgccgc
      661 cccgccggag acggggcggc agcaagggag ataccgtgat gaaaactgaa ctgaccctga
      721 atgtattaca gaccatgagc gcacaggaat atgaagatat ccgggctgcc ggaagtgatg
      781 aacgccgtga gctgacacac gccgtgatgc gggaactgga tgcgccggac aactggacga
      841 tgaatggcga gtacggcagc gagttcggcg gctttttccc cgtccaggtc cgtttcacgc
      901 ctgcccacga acgttttcac ctggcgttat gttcaccggg cgatgtctct caggtctggg
      961 tgctggttct ggtgaatgcc ggtggtgagc cgtttgcggt ggttcaggtg cagcggcggt
     1021 ttgcaccgga agctgtcagc cattcactgg cactggcggc atcactggat gaacagggat
     1081 acagcgtcag tgacatcatc catatcctga tggcggaagg aggtcaggca tgagcgcacg
     1141 ttcacaggca ctggttcctc tcagcacaga gcaacaggcc gcatggcggg cggtggcgga
     1201 gacagaaaaa cgccgccacc agggtaacac gctggcggaa tatccgtatg cgggggcgtt
     1261 tttccgctgt ctgaacggaa gccgcaggat ttcgctgtct gacctgcgat ttttcatgcc
     1321 gtccctgacc gcagaagaac tgcgcggcaa ccgttctcag tggctgtatg ccgtcgatgt
     1381 gctgattgaa acacagggcg aggtctgcct ccttcccctt cccggtgatg cagcagagcg
     1441 gttgttcccg tcggtgcggt tccgtgtcag ggaacgcagt cgccataaat ccgtgctggt
     1501 catgcagaag tacagccgcc agcaggcacg ggaggcagag cagaaagccc gtgcgtacca
     1561 gacgctggtc gctcaggctg aaatcgaact ggcgttccat tcaccggaaa cagtcggaag
     1621 ctggtatgca cgctggagtg accgggtggc agagcatgac ctggaaaccc tgttctggca
     1681 gtggggtgag cgttttccgt cactggccgg gatggaacgc tggcagtggc aggatatgcc
     1741 attctggcag gtcatcgcag aggcaggtat ggcagcaagg gaggcgggtc acgctgtccg
     1801 tgagatggag cggtggatgg tgccgaacaa actccgggag gcggcgtgaa gtggcgttac
     1861 tcattgcgct ggaaactgcc ttctccctgt cctggcgagc atgaactggt ttcagaagtg
     1921 gtggaggcgg ggcagcctgc accggcatcc gttatgtcac gctgggttgc cggagcaggg
     1981 tatgcggtct gtctggattt tatttctgac cgcccggtgc gccgctggag cgaagaacgt
     2041 aaggctgcgg tacggcggcg gaatctggag aaacgtatca acaggcatgc accactgttt
     2101 gcggatgaac tgattgcccg tgaactggct gaacgaccgg attattttca gggaaagtga
     2161 gtgtggtcag tacgcaggta tatgggctat gatgtgcccg gcgcttgagg ctttctgcct
     2221 catgacgtga aggtggtttg ttatcctgtc gtgtggcaga aggacaaaag ccccgtagtt
     2281 aatttttcat taacccacga ggcctctgca tgtctagtcc acgtcagtat ggcctcttac
     2341 cgtgcaaatt gcaaggagga gtaaaccatg aaactaccgg gaaacgccct tatctggtgc
     2401 gtattaatcg tgtgctgcac gttgttgata ttcacactcc tgacccggaa tcgcctgtgc
     2461 gaagtccggc tgaaggacgg atacagggaa gttacggcaa caatggctta cgaatccggc
     2521 ggtaagtagc aacctggagg cgggcgcagg cccgcctttt cagggctgat gctggtctga
     2581 ctgcactgaa gcgcctttat aaaggggctg ctggttcgcc ggtggcccct ttctccttgc
     2641 tggcattgta gcagcatgaa caaccagact gtcaggaagg ccacttccct ccgggaacgg
     2701 gaatgaacgt gcgcctgcgg cgctcgttct tttcccccgc cttctctgtt tatggccagt
     2761 tgtcagaaat gacattacct ttcccgtgat tattctcttt ttcttcctgc tctgattctg
     2821 actactgcag tgttgtcctg tctgccgggc gcaccttcgg ctcccggctg cctggcggca
     2881 tccggtcgtc aggcccgact ccgacaggca gctgctggcg cacctgccag tctccgccga
     2941 acccgctggc gcggtttcgg gctgccgcta cggtccgatg cgtgaaaacc ccgggcttgc
     3001 agcgaccgtt ccggtcgcgc cgcctctgtc gcgccgtggc ctgcgctccc ggcatcccgg
     3061 caaaaagacg ccgggatgta aggagcaacg ccgtttcgca aaccgcgctt cacgtcgttg
     3121 cggcgggtcc ctgcccgttg ttctgtccgg tgccattcac ctgtttaagc ccctgtcgcc
     3181 gggggctgat acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca
     3241 aaatttttta cccaaaaccc ggtaaaaaat tttgcgttct gcccaggaca ggtgcgtcag
     3301 gccgtggcag tgatgcccca tgcggcgaag gttctcaaac aggtgatggt gaataccgga
     3361 gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat
     3421 gatgggggct gaaaacatca gatacaggag gaaacatgag cgcattttct tttaacaccg
     3481 cttatcagcc aacaggcgat cagcagaaag acgcaatcgc acaaatcgac atcatgcaga
     3541 atcgtgctgt tcaggcaaat cttgcatacc agagcagcct ggatgcggaa acgctattga
     3601 aacagttatg gcaaatcaat gatgaactgg gggaactgtt ggatagcgtg gaaaatcaca
     3661 cgccagttat cagaaaaaaa gccagtgatt tgagcgcact gatgatgctt ttcagccaac
     3721 aggccggtca gcctatgact atgttgttga tttgttaatg ataatcaggg gcaggaaatt
     3781 aagcgaaaga tgctctgccc cgggtttgca gcgaccggaa cggtcacgcc acccttgtcg
     3841 cgctgtggcc tgcggcggct cccgaaactc aaccgatgat gggggctgaa aaccagagag
     3901 aggagaaagc aggatgagta cccgaaatat ccacgttaac accgcgtcgt acacgcttct
     3961 tgttgccggg aagaaaaaaa acaccgggga agagtgggac gtcctggaat tcagcagcct
     4021 gactgagctg aaaaaatatc gcaaaagcca cccggaaaag atggccttca gctatagcta
     4081 cgcgctcagc cggggtgtgg atacgcagtt ccgccatatc aacattgcgg aagccgatca
     4141 cttcaaacag ttcctgcgtc agattaagcg taccggcctc gatatccggg cgatctgctg
     4201 accggatgtg atgaatatcc ctcctgattg ttgcaatctt gttaatggag accgggaggg
     4261 atgatattaa gcggaaaatt ttttattaaa tttattcttt cataaaggag tagctgttat
     4321 gcgattagct tcccgttttg gtcggtataa ttccattcgc cgtgagcgtc ctttaacgga
     4381 tgatgaatta atgcagtttg tgccttcggt attttccggt gataaacatg aatcccggag
     4441 tgagcgttat acatatattc caacaatcaa tatcatcaat aagttacgtg aagaaggttt
     4501 ccagccgttc tttgcatgtc agagtcgggt tcgggatttg ggacgccgcg aatacagtaa
     4561 acatatgtta cgtcttcgca gggaagggca tattaacggg caggaagttc ctgaaattat
     4621 cctgcttaat tcacatgatg gttcatccag ttatcagatg atccccggaa tttttcgttt
     4681 tgtctgcaca aatggtttgg tgtgcggaaa taattttggc gaaatccgcg ttccacataa
     4741 aggcgatatt gtcgggcagg ttattgaggg agcgtatgaa gtgctcggtg tctttgataa
     4801 ggtcactgaa aatatggagg cgatgaaaga aatacatctt aacagtgacg agcaacattt
     4861 atttggcaga gctgcactga tggtcaggta tgaagatgaa aataaaacac cagtgacgcc
     4921 tgaacaaata attactcccc gtcgtttgga ggataaacag aacgatctct ggacaacctg
     4981 gcagcgggtt caggagaata tgataaaagg tggactgtcg gggcgaagtg cctccggtaa
     5041 aaataccagg acaagaggca ttacgggtat tgatggtgat atccgaatca acaaagcatt
     5101 atggatgatt gccgaacagt tcagggagtg gaagtcatga taatataagt aaagccccga
     5161 aaattttttc ggggctttac ttatattatc attatgcggc attgatatgt cgtctgagat
     5221 gcctcaattt catcaactgg cttttcggta gtctgtaaat attgtgcccg cgaaaaatgg
     5281 tctggcagga ggctgcgcag ttaccggata tatataagat gggttcccgc tttgcggggc
     5341 ttcggccagt cagcattatc actcatatga tatttttgtg tgtggcctta catgccgcgt
     5401 ttgcggcata accggttttt ttaaaatgta tatcaaatta attcttctgc gagctgttca
     5461 tttttgaaag cggtttttca gatgcacgaa gctggatccc cttgctgttt tttatcccgc
     5521 tgtaaatccg gtaaacatct gatgcatagg caagacgccg ctggttctga cgttcggttt
     5581 tcctgaatcc tgcgttgtat gcaccaacag cctcccagga gactccccat tttttaaagg
     5641 ctatagccag ataataagca ccggtataaa tgttcagaca gggatctgct gtcagatgtt
     5701 ccggtttaat tccgtagcgg gccagttcgt taaaatgctg ggaatctacc tgcataagtc
     5761 cactgccata tcccgtaacc ggattaatac cgatggcatt aacccggtta cgggattctt
     5821 tccatgatat tgctctcagc aagtcagggt caattttata atctctgcca gccagatcaa
     5881 agcaatcacc agccttgcag gtcacattta taaacatcag gcagatcgtc agaagccatt
     5941 ttctcatttt ctcacctctg gtgacgtcat caggaaataa tttaacccac tccacaaaaa
     6001 ggctcaacag gttggtggtt ctcaccacca aaagcaccac acactacgca aaaacaagtt
     6061 tttgctgatt tgcgttttaa atcatgcaat taacaaatgc tatttatatt taataattct
     6121 gaattattaa atagagagtc gttggcgatc ctgttacgac cctatggcga cccttattag
     6181 gatcagtact gacaattttc atgaaaaaca tcgatatata ttaggggcac ccctagcgcc
     6241 gggtcgcatt tttttatagg atgatcctag aggcgctgct aggagtgtct ttcttacttg
     6301 taaacactat atgcatattt atagtgaaaa gttcattgct taatgatgaa aggttttatc
     6361 ttatggccag agtaattttg tatatcagta atgatgtcta tgataaagta aatgcgatag
     6421 ttgaacagcg tcgtcaggag ggcgcaagag ataaagatat aagcgtatcg ggaactgctt
     6481 caatgcttct tgaactaggg cttcgtgtat atgaagctca gatggaacgt aaagagtctg
     6541 cgtttaatca gaaagagttt aataaacttc ttcttgaatg tgttgtaaaa acacagtcat
     6601 cagtagcgaa aattttgggt attgagtctc taagtcccca tgtctccggg
//
LOCUS       DAZGAP010000233         6530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGAP010000233 DAZGAP010000000
VERSION     DAZGAP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.234"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..504
                     /locus_tag="ACHLAM_004647"
     CDS             <1..504
                     /locus_tag="ACHLAM_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856069.1"
                     /translation="NQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTRPDVKNR
                     EIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTK
                     MIYLTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILGFFLSIVISVMFCLVKKMVDE
                     YQQNSGQ"
     gene            complement(745..1440)
                     /locus_tag="ACHLAM_004648"
     CDS             complement(745..1440)
                     /locus_tag="ACHLAM_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO7856070.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERFTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1433..2860)
                     /locus_tag="ACHLAM_004649"
     CDS             complement(1433..2860)
                     /locus_tag="ACHLAM_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO7856071.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2871..3590)
                     /locus_tag="ACHLAM_004650"
     CDS             complement(2871..3590)
                     /locus_tag="ACHLAM_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO7856072.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGLKVKVMHIAEVLMSR"
     gene            complement(4118..4972)
                     /gene="rclR"
                     /locus_tag="ACHLAM_004651"
     CDS             complement(4118..4972)
                     /gene="rclR"
                     /locus_tag="ACHLAM_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO7856073.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5198..6523
                     /gene="rclA"
                     /locus_tag="ACHLAM_004652"
     CDS             5198..6523
                     /gene="rclA"
                     /locus_tag="ACHLAM_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO7856074.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1773 a         1617 c         1422 g         1718 t
ORIGIN      
        1 aaccagacaa caccaggcat acttgttagc tgtgatagca ataataattt tgcctgtcag
       61 accacaatga ctgaagatgt tattcagcga attactacat tttttcagac cagaccagat
      121 gtcaagaaca gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct
      181 gaagcggaaa tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat
      241 aatggcaggc aagttcttga tgagatacaa acgccttcag caattaacag tgagctttat
      301 acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta
      361 accataagct caccggaaat aaaaagtaaa taccctgctg ccatttgtct gattctggga
      421 ttttttctaa gcattgtgat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa
      481 taccaacaaa actctgggca gtaataaaca gcattctgac agagagtgcc tgtataaaac
      541 tatagtgaca atgaccggga agggagcccg tcttcaggaa tggattcctg tctttttcac
      601 tgtaaatatc ctggcattat taatgtcagg atatttacag aagagcagca taaccatgac
      661 ggaacggagt taagtcaggt tgtcattagc gctattcgca acctcgtgcc gccttcaggt
      721 ttatgtccag acttcatatc tctctcagca atcctcaata atcagataca ccgctttcac
      781 cggaccatga accccgacga ctttgataag ctcaatgtcc gccgttgaac tgggaccact
      841 gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat gcaatttttc
      901 tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa gagaagattc
      961 cgggagcagg ctcaatgaac gcccgcgccc ggcggcggaa aaaagaacca cgcctcccga
     1021 ttcggttaaa ccatattcag catatacgac gcccacttta gcctgctctg cttgtgatat
     1081 gttctcaata cctttcgccg gatcccaaac aacggcattg cattcctgct gcaaacgttc
     1141 gctaatcccc aattcctcca acctcgtgtc accactaatc acgaccgact gatctcccag
     1201 ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca gttcacagcg
     1261 cgtcaacata acatcgctgg caaactggat aaacgcgtca cagcgctgct gttgggtaag
     1321 ttgggtaaac cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag
     1381 tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc gattatccat
     1441 tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt
     1501 caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc
     1561 cgccattgat aaaccagctt gccgcatgag caccagccat catcccaact ttccacaatc
     1621 ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga
     1681 tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca
     1741 ccggacacac gctgtcacaa gcggtgcata aagagcaggc gtagggtaaa tctttaaaat
     1801 ctttatagcc gccaagtaac ggggaaatca ccgcaccaat tggccctgga taaatagagc
     1861 catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcaac
     1921 gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga
     1981 caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc ggtcctgtca
     2041 gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca
     2101 atacatctac ctctgcaaac gtgggagcaa tacgctccat tcccatcact gcaatatgcg
     2161 ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac
     2221 cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt
     2281 cgcggatttt ttgccgaatg aataaggtca tcgcttcagg cgtttccggc ccctcatagc
     2341 ccagacgttc gtgtagcacc cgacggatct gatggcgatc tttatgaatt gccgggacca
     2401 caacatgaga gggcggatct tgatccagct ggagaatgta ttcacccaga tcggtttcaa
     2461 tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg
     2521 atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg
     2581 tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc actttttctg
     2641 agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc
     2701 gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccga
     2761 tacgctgctg cgcgtttgcc accgctttgc gcatgatcgg atcttcaatt tgctgacgga
     2821 tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc
     2881 atcaacactt cagcaatatg catcactttg actttcagcc cttcccgttg taatcgtcca
     2941 ctgatgttca gcaggcaact cacgtcagca cctattaaat actcagggcg gacttccatc
     3001 aggtgcgcaa ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac
     3061 gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc
     3121 acatttttca gcagcgtaag tggttcgtcc ttcaccccca gcttacgggc caggctacaa
     3181 gatgggtgat acaccgctct cccttgcaaa ctggcaccta catcgactac ccctaatgta
     3241 ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc
     3301 cattcaggtt catccgccag atacgtcggg taacttttta cggcataggt gcaagagcca
     3361 gctggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct
     3421 ggaatcgctt ctttgatata accactattg atcgcaggct gaccgcagca tccctgtttc
     3481 tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt
     3541 cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact
     3601 ccattacttc atgcccattt atgcccgatg gttcccccct tccgggggat caatcatcaa
     3661 taaatatggc gttctttttt aattggctag caacatacca cccctcattt gtatgacaaa
     3721 tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct
     3781 cacattttat agggaaatcg acgatcattt ttggcataag aaatccattt aaaaaggcta
     3841 catcccttat acctattcag tgctacttat tcaggggtta ttgagatata cactgatggt
     3901 gtacaaacaa aatcaccaac aataattacg tatcaagtaa tagtcggcat tttatttaat
     3961 taaagcaatt tagtttgcgt ttttatttaa aaactgagct agagtccatc aaaaaaaata
     4021 tataaagccc aaaaccaccg ttggaaatag aaatagttga aaggctcaca actcccggaa
     4081 tgcggattat ctttaccctg atttctggcg gttttattca gggtgcaagc tgtctcaccc
     4141 tttcccgata ttctcccgga gtacaaccaa actcgcggac aaacgccttg tgaaaagatg
     4201 attcactggc atagcctact gactcagcga tcaccacaac agggagcatt tcccgggaaa
     4261 acatctgggc cgctatttgt agacgcaact ttgttaatac agccagcggc gtggttccgg
     4321 aaacatcacg gaaaagctgg gcaaaacttg cccgggacat gtgggcgatg ctggccagcg
     4381 attcgacggt ccaggcgtgt cctggcattt ccagcatttg ctgtattacc gcaccaagac
     4441 gtggatgcag aagcaaactg agaatgtttt tctctgtatt aacctgtgca atccactcac
     4501 gcaccgcgag ggtaaagaat gtagcgcaga tctggctaca cagagcatcc actcccggca
     4561 ttgccgatct ggattcctgt tgtaaaaacg ggatcgcctc cctcagccag ttatattcca
     4621 cgctatggtt cactggtgct acaaacagcg tttccggcaa agatgttaaa aaataacgcg
     4681 ccgaatgttg cagccgaaga gtgccacaga caatacaggt cgattcatta tcgacatgac
     4741 tcaggcgatg agctgaattt tgtggtagca ggaccacatt tcccgggcgt aatgtaaaaa
     4801 tctcccccgt cggcatttcc agcttcgccg ctccttgcgt taacgcatgc caacgaataa
     4861 ccgacaattc cccggcacca tgtggaagtt gccagtcact tcctaacacg caattcttat
     4921 cgatcgttcc ttgtggagcg ttaagcatca acaaacggct aagggcatcc atatcagact
     4981 cctgagtggt ttttgattac aaacaccaac aatcttagac aataattaat ctaatatcat
     5041 caaatggtta atcatgattt ttgttttgtg attctgatta agaaaaagaa aataccagac
     5101 gataagacaa aatatcaagc gttgtgaaga aatgttattt gctcttttgc gtctatagtc
     5161 atgatgtcaa atgaacgcgt ttcgacagga aatcatcatg aataaatatc aggcagttat
     5221 tattggtttt ggcaaggctg gaaaaacatt agccgtcacg ctggcaaaag caggttggcg
     5281 tgtggctctc atcgaacaat caaatgcaat gtatggcggg acctgtatta atatcggctg
     5341 tatcccaacc aaaacattgg ttcatgacgc acagcagcac acagattttg tccgtgccat
     5401 acagcgtaaa aatgaagtgg ttaatttttt acgtaataag aattttcata atcttgcgga
     5461 tatacccaat atcgacgtga tcgacggcca ggcggagttt atcaataatc atagcctgcg
     5521 tgttcatcgg cctgggggaa atctggagat tcatggcgag aaaattttta ttaataccgg
     5581 tgcacaagcc gtggttccgc caattcctgg aattaccacc acgcctggtg tatatgacag
     5641 caccggatta cttaatctaa aagaattgcc tgggcattta ggtattttgg gcggcggata
     5701 tattggcgtt gagttcgcct ctatgttcgc taattttggc agcaaagtaa ccattttaga
     5761 ggcagcttcg ctgtttttgc ctcgggaaga tcgggatatt gctgataata tcgcgacgat
     5821 tttacgcgat cagggtgtcg atataatcct caatgcccat gtggagcgaa tcagtcacca
     5881 tgaaaatcaa gtgcaagtgc atagcgagca cgcccaactg gcggtggatg cactgttaat
     5941 agcttccggt cgtcaaccag ctaccgcttc gttacatcca gaaaatgccg gtatcgcagt
     6001 aaacgagcgc ggggcaattg tcgttgacaa gcgattacat accaccgcag acaatatttg
     6061 ggcgatggga gatgttaccg gcgggctgca atttacttac atatcactag atgattaccg
     6121 cattgtacgt gatgagttac tgggtgaagg caaacgtagt actgatgatc ggaaaaatgt
     6181 gccttattcc gtatttatga caccgcccct gtccagggtt ggtatgacag aagaacaagc
     6241 cagagagagt ggtgctgata ttcaggtggt gacattgcct gtagctgcaa ttccgcgcgc
     6301 cagagtgatg aacgataccc gtggggtatt aaaagcgatt gttgataata aaacccaacg
     6361 tatattaggg gcatcactgc tatgtgttga ctcccacgag atgatcaata tagtgaaaat
     6421 ggtgatggat gccgggctgc cttatagcat attacgcgat cagatattta ctcatccgtc
     6481 gatgagcgaa tcactcaatg atctattttc attagtcaaa taaactcaaa
//
LOCUS       DAZGAP010000234         6507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGAP010000234 DAZGAP010000000
VERSION     DAZGAP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.235"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..813)
                     /locus_tag="ACHLAM_004653"
     CDS             complement(<1..813)
                     /locus_tag="ACHLAM_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFO7856075.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGSAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFF"
     gene            complement(810..1838)
                     /locus_tag="ACHLAM_004654"
     CDS             complement(810..1838)
                     /locus_tag="ACHLAM_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7856076.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLES
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            1935..2750
                     /locus_tag="ACHLAM_004655"
     CDS             1935..2750
                     /locus_tag="ACHLAM_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7856077.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(2924..3140)
                     /locus_tag="ACHLAM_004656"
                     /pseudo
     CDS             complement(2924..3140)
                     /locus_tag="ACHLAM_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3140..3283
                     /locus_tag="ACHLAM_004657"
                     /pseudo
     CDS             3140..3283
                     /locus_tag="ACHLAM_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3294..3809
                     /gene="ogt"
                     /locus_tag="ACHLAM_004658"
     CDS             3294..3809
                     /gene="ogt"
                     /locus_tag="ACHLAM_004658"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO7856078.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            4004..4756
                     /gene="fnr"
                     /locus_tag="ACHLAM_004659"
     CDS             4004..4756
                     /gene="fnr"
                     /locus_tag="ACHLAM_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO7856079.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            4908..5858
                     /gene="uspE"
                     /locus_tag="ACHLAM_004660"
     CDS             4908..5858
                     /gene="uspE"
                     /locus_tag="ACHLAM_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO7856080.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5908..6165)
                     /gene="ynaJ"
                     /locus_tag="ACHLAM_004661"
     CDS             complement(5908..6165)
                     /gene="ynaJ"
                     /locus_tag="ACHLAM_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO7856081.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            6409..>6507
                     /locus_tag="ACHLAM_004662"
     CDS             6409..>6507
                     /locus_tag="ACHLAM_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856082.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRG"
BASE COUNT         1785 a         1558 c         1458 g         1706 t
ORIGIN      
        1 aaaaaaacat cccagcgtga aatttttatc cttaaaagca tgaagatcca ttatcggatt
       61 ggatattgta agtgtacgcg cagcaaataa aataaaactc accaatgcca gaactgatgt
      121 cagcaaaata gtattatcat ccagccatcc ccagcgcgct ccttcttcaa gggtatattc
      181 cagacatccc agtgtcatgg ccaacaggat aatactcgga taatcagcaa ctttcagtaa
      241 agagagatca ggtttatcaa aattaaccag aaaaggaata ctcaaaacta aatatatgcc
      301 aggtaatata ttgatataaa atagccatcg ccagtcgagg ttatcagtga tccagccccc
      361 cagagttggc cctagcgtcg gtgacagtga ggccagcgca ctgactaccg cagcagccga
      421 ccccaactct ttaccctggt aataaataaa cgccgtcgtg aaaactaaag ggatcatgga
      481 tgcgccagcc gctccctgta aagcacgaaa caaaatcatg atctgaatat tccatgcaag
      541 tccacacgcg atactcatta aagtaaatat tcccgcggat agagtaaaaa gccaacgagt
      601 ggaaaatacc cgtgacagcc acccagagag cggaataaca ataatctcag caattaaata
      661 gctggtctga agccaggcca tttcgtcttt acctgcggac agaccaccac ctatttcatt
      721 tagggatgac gatacaattt gaatatcaag ctgagcaata aataatccca tacacatact
      781 ggcgaaagca atatgtttac gcatagacat catctctcat cgacactgac ggtgacggac
      841 agtcccgggc gaagaagttg ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg
      901 acacgctgaa caattttggt gaaattgcca gtggcatttt ctatcggaat caagctgaaa
      961 ctggctcctg tcgctgggga aaggctttca atgtgaccgt gaaacacttc ccctttgaga
     1021 atatcagcac ggatctcagc cttcattcct ggcttcattc ccgctatttg gttttcttta
     1081 taatttgcat caacccacaa ctcactgact ggtacaagtg aaactaacga tgtgccgcct
     1141 tccacccaac tgcctgtatg ggcgctgcgg tttgcgacaa tgccatcaat cggcgcacga
     1201 acaagggtat attccagatc cagtagtgcc tgagtcaaag cggcctgaac ttcttcgata
     1261 ctcgcacgta cgttttcttc ctgagcagat aaaactgcca gccgttgctt ctccaccagg
     1321 aaatcgttcg cggctttgcg ctcctttgct gcgacctgct ggtagtcaaa cctggcgtta
     1381 tcaataattt gctgcgatat tgccgctgat tgtacgagtt tttcataacg ttcagtatct
     1441 cgcaggcgct tttgctcctc atgttttact gcctgccatg tttcactggc actttgaatt
     1501 atcgactgct gcatcgtaat agtggcttga atgctttcga gattggcttt tctacttttt
     1561 atttttgcct caagcctggc gacatttgca cgataatccc gatcatcgag acgcaggata
     1621 atatcgcctt tttttacgct ctgattatca cgcacctcta tagcagaaat ataaccagaa
     1681 actttacttg ccaccgtggt aatattacca ccaatgtaag catcatccgt cgtctgtata
     1741 aatcttcctt tgcttaccca ccagataaaa aagacaacgc cagccaaaat aataccgatc
     1801 gctactaccc caataagctg ttttttagaa attatcatca ctctttacac cgtatatatc
     1861 cggaaacttg cattcgccat atttaaaatt taacatggct tatttgatga tttctgacaa
     1921 aatacctact gtaaatgaca atcacactgc aacccaaagc atcaccaggg catcacatta
     1981 tcggactgga ttcggaacat ctgaatggag gcacagttcc ctggcataaa catctctatg
     2041 cccagttgct ttacccggct gaaggagtcg ttcgtgtatg ggctggagaa agcgtctggc
     2101 tagtgcatgc cagcagcgcg ctttggctgc ccccacaaat gccgcataag tttgttgcga
     2161 caggcaacgt acttttaaaa accgtactgg tttcagaagc ggaaagtgaa acgctgggaa
     2221 aagtttgctt catgacgggt atttcacccc tgttaagaga attactaatt gcaattaacc
     2281 aacttccacc gtcgcaaagt accacagata aacaacagtt acgtttttca gccctcgaga
     2341 cactgatact tcaggaaata aaaatggggg tgaaaatgtc acttgaactc ccttggccaa
     2401 atgatgagcg gctacagcag ctttgtgaga atttgctaaa caaccaaggg tatctgccaa
     2461 cactggataa tctagcggat aaaattaacg tcagtagccg tacactcatg cgcttatttg
     2521 ttaaagagac gggattaaca tttcgccact gggttcagca aatgcacgtt atttctgcag
     2581 tgacgcttct tgatgatgga tattcgttga ccaaaattgc ccatcgtctg ggctatgcct
     2641 cagctgaatc gttcggcaat atgtttaagc gtcgaacggg ctactcgccc ggtaaattta
     2701 ctcgtcgatt aacgatgcat gattatgcaa tcacaaggca aatgatttaa cttatatttt
     2761 cgtgaaatct gtcactgaag aaaattggca acggatgggt aaaaccgtta taacactgtc
     2821 accggtcacc aggaccccag gccggattta agacgaggat gcactgctgt gtgtactgta
     2881 gagtctggcg gatgtcgaca ggctctattt ttttattcat tttttagctc tgtaaataac
     2941 cacattctcg ccgggattgg ggaatgagtg acgagatcag tggtgtctgg cggctatcac
     3001 gccacagaat caaataacag atagcctacg gtaaaatttc gctgactgga attatcccac
     3061 cctcgatcca tgtgatggct tgccctcatt tcttcaggca gaaaaatcaa aaaatcgctt
     3121 tatgctatca gactgcgaca aaccagacgc gaaactgggt acttactatt cgttagtctt
     3181 gccctatccg cttatctttt tggtggtatg gctgctgatg ttgctggcgt ggtatcttgt
     3241 tggtctgccg ataggtccgg ggatttaccc acgtttgtct taagagagaa cggatgctga
     3301 gattacttga agaaaaaatt gccacgccac tgggtccact atgggtgatt tgcgatgagc
     3361 aatttcgcct gcgggcggtt gaatgggaag agtacagcga acgcatggtg cagctgctgg
     3421 acatccatta tcgcaaagaa ggctatgagc gcatttctgc cgccaaccca ggtggtttaa
     3481 gcgacaagct tcgtgaatat tttgccggta atcttagcat tattgatacg cttcccactg
     3541 ccacgggggg gacgccattt cagcgcgaag tctggaaaac actacgcact atcccctgcg
     3601 ggcaggtaat gcattacggt caactggccg aacaattggg tcgccctggc gcggcgcgtg
     3661 ccgttggtgc ggctaacgga tcgaatccca tcagcattgt tgtaccttgc catcgggtta
     3721 ttggccgaaa cggcaccatg accggatatg caggcggagt tcagcgaaaa gagtggttat
     3781 tgcgccacga aggttatctt ttgctgtaaa cattaaacaa tttgtgccag cttgttcaca
     3841 cttttatgta aagttaccct taacaactta agggttttca aatagataga catatattta
     3901 catctaatat cggaattctc tgctgttaag gtttgcttag acttacttgc tccctaaaaa
     3961 gatgttaaaa ttgacaaata tcaattacgg cttgagcaga cctatgatcc cggaaaagcg
     4021 aattatacgg cgcattcagt ctggcggttg tgctatccat tgccaggatt gcagcatcag
     4081 ccagctttgc atcccgttca cactcaacga acatgagctt gatcagcttg ataatatcat
     4141 tgagcggaag aagcctattc agaaaggcca gacgctgttt aaggctggtg atgaacttaa
     4201 atcgctttat gccatccgct ccggtacgat taaaagttat accatcactg agcaaggcga
     4261 cgagcaaatc actggtttcc atttagcagg cgacctggtg ggatttgacg ccatcggcag
     4321 cggccatcac ccgagcttcg cgcaggcgct ggaaacctcg atggtatgtg aaatcccgtt
     4381 cgaaacgctg gacgatttgt ccggtaaaat gccgaatctg cgtcagcaga tgatgcgtct
     4441 gatgagcggt gaaatcaaag gcgatcagga catgatcctg ctgttgtcga agaaaaatgc
     4501 cgaggaacgt ctggctgcat tcatctacaa cctgtcccgt cgttttgccc aacgcggctt
     4561 ctcccctcgt gaattccgcc tgacgatgac tcgtggcgat attggtaact atctgggcct
     4621 gacggtagaa accatcagcc gtctgctggg tcgcttccag aaaagcggta tgctggcagt
     4681 caaaggtaaa tacatcacca tcgaaaataa cgatgcgctg gcccagcttg ctggtcatac
     4741 gcgtaacgtt gcctgatttt tccgcataac tcactatcct tctgtcatat cattaaattt
     4801 ttctgattta ttgatctggc agaaggttca tcactgtttc attcaccaga tatgggttaa
     4861 tcttttaatt acaaactgcg ttgacagttg ttgtaaggag accctgtatg gctatgtatc
     4921 agaacatgct cgttgttatc gatcctaacc aggacgacca accagcattg cggcgagctg
     4981 tttatttaca tcaacggatt ggtggcaaaa ttaaagcctt tttgccgatc tatgacttct
     5041 catacgaaat gaccaccctg ctctccccgg acgaacgtac cgctatgcgt cagggcgtca
     5101 tcagccagcg tacagcctgg atccacgagc aggcaaaata ttatctcaat gctggcgttc
     5161 ccattgaaat taaagtggtc tggcataacc gtcctttcga agccatcatt caggaagtga
     5221 tcagcggcgg gcacgatttg gtgctaaaaa tggcgcacca acatgaccgt ctggaagcgg
     5281 tgatttttac gccaacggac tggcatctgt tacgcaaatg cccaagcccc gtgtggatgg
     5341 tgaaagacca gccgtggccg gaagggggta aggcgctggt ggcggtgaat ctcgccagtg
     5401 aagagccgta tcataatgcg ctcaatgaaa aactggtcaa agagacgatc gaactggcgg
     5461 aacaagtcaa ccataccgaa gttcatctgg ttggcgctta tccggtaacg ccaattaata
     5521 tcgcgattga actgccggaa tttgatccga gcgtttataa cgatgccatt cgtgggcaac
     5581 atttgctggc aatgaaagcc ctgcggcaga aattcggcat taatgaaaac atgacgcacg
     5641 tagaaaaagg tctgccagaa gaggtgattc ctgatttggc ggagcactta caggcgggga
     5701 ttgtggttct gggcacggtc ggacgcaccg gtatttcagc agcattcctt ggtaacacgg
     5761 cggaacaggt gattgatcat cttcgctgcg acctgctggt cattaaacct gaccagtatc
     5821 agacacccgt tgaactggat gacgaagaag acgattaacg actcttcaat aactgacaag
     5881 gcccgagtta tcgggcctat tttttactta ctgctcttcg ccgccaagga aataaattcc
     5941 caacgggatt gccagcaaca cagtaaagac caggctataa acaaaaatca tcgatgactg
     6001 aatcacatac attgatgtcg tccactcaga cagcggaata ttgtactgtt ctatcacgcc
     6061 gccgatggtc gcgcgagtca caaccgacgc cgcaataatg aaaaccgcca acaaaccaaa
     6121 gagaaatttc ttccctttcg ctgacttcag tttcgccata atcatgatgt aatccttgtg
     6181 cagacaaatt tcttgttacc taaacgtaac aataccatga catttcaccg ttgtctgctc
     6241 tgaaaacctc acctcttagc tggatttaca atcaacgcat tgattagtta gctaatgttg
     6301 ctacttcctt ccctgactat tgatgatctg aatgaattgt cgcggtaaaa tagccccgcc
     6361 aggggtttcc ggcatgacga caattatctg gaggagcctg ataaaacaat gatcgctgaa
     6421 ctgtttacaa ataatgcgct taatctggtc attattttcg gtagctgcgc agcattgatt
     6481 ctgatgagct tttggtttcg ccgcgga
//
LOCUS       DAZGAP010000235         6497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGAP010000235 DAZGAP010000000
VERSION     DAZGAP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.236"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..267)
                     /locus_tag="ACHLAM_004663"
     CDS             complement(<1..267)
                     /locus_tag="ACHLAM_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO7856083.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGG"
     gene            complement(264..1934)
                     /gene="menD"
                     /locus_tag="ACHLAM_004664"
     CDS             complement(264..1934)
                     /gene="menD"
                     /locus_tag="ACHLAM_004664"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO7856084.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2023..3318)
                     /gene="menF"
                     /locus_tag="ACHLAM_004665"
     CDS             complement(2023..3318)
                     /gene="menF"
                     /locus_tag="ACHLAM_004665"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO7856085.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3397..3702)
                     /gene="elaB"
                     /locus_tag="ACHLAM_004666"
     CDS             complement(3397..3702)
                     /gene="elaB"
                     /locus_tag="ACHLAM_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO7856086.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3757..4218)
                     /gene="elaA"
                     /locus_tag="ACHLAM_004667"
     CDS             complement(3757..4218)
                     /gene="elaA"
                     /locus_tag="ACHLAM_004667"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7856087.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4283..5200
                     /gene="rbn"
                     /locus_tag="ACHLAM_004668"
     CDS             4283..5200
                     /gene="rbn"
                     /locus_tag="ACHLAM_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO7856088.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5388..5501
                     /locus_tag="ACHLAM_004669"
                     /pseudo
     CDS             5388..5501
                     /locus_tag="ACHLAM_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            5556..>6497
                     /gene="yfbL"
                     /locus_tag="ACHLAM_004670"
     CDS             5556..>6497
                     /gene="yfbL"
                     /locus_tag="ACHLAM_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO7856089.1"
                     /translation="MKKMTSLCFIPLLLSLTFIIFYQPWVRALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDG"
BASE COUNT         1574 a         1742 c         1583 g         1598 t
ORIGIN      
        1 tccgccaagc gagtacccca ccagccagaa gttaaggatg ttgtaactaa ctaaggtttt
       61 acgcagtaag tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccgtg
      121 acctgggaga tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca
      181 ttcatggcaa tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggtttacc
      241 gtgttttgcc tgcgcgtgca ggatcataaa tggcttacct gcgccagcag ttgctggagg
      301 gtttgcgcac catcggtgtc gttaaccacc atttcaatta ccgtggtggt cggcgtgcgc
      361 caggcgtcgg caagtgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc
      421 tcgaacatcg cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca
      481 cgctcgtttt tcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca
      541 attaatacca gcggcgcgga aacctgacgt aataacgcca gcgcgttgag atcgtaaagt
      601 gctgagagat cgcccacaat cgccagcgtc ggtttgccac tggcccgctg aacgccagcg
      661 gcggtcgaga gcagcccgtc gataccgctg gcaccacggt tgctgtacac cgggtaacct
      721 gccggaagtt gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat
      781 tgaccctgtt caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca
      841 tcgcgacggg caataaccgc ctgcattgcc tgttccgcca ggcgcggaat ttcaacgcac
      901 cagggctggc gtttttctgc cggatgctgc tccagccagt cggcaatatt ggcaattaag
      961 cgacgtccac gatggtgtgc cggatcaagt cgcccttcga tgtcatcaac aatccagtac
     1021 tcttccggtt cacagcttgc ctgccattgc aggagccgtt tgcccgtcag gctgcttccc
     1081 agttgcacca caatttgtgc ctgttgcagt tcgctggtcg ctttggcatt gcctaaccag
     1141 agatcggcac acggcagcgg ctgtccggtt tgcgacagca cgtcgccaat cagcggccag
     1201 ccaagagttt gcgcccacag tgcaactttt ttgccctctt ccgcactcat gcgcccggca
     1261 accaccacgc cgcgcttttg ccgccagaag aaccagtcgc gctgtttttc actttccaga
     1321 cgaggcgctt cacgcagcca cggtttgtcg tcctgccacc agtcgcccag ccgctgttgc
     1381 cagctaagcc cggtgtcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata
     1441 tggactccgc ccgcatgaag cgtaccgaga gcatggtcga tggtagaaac cagccaacgt
     1501 gcaggaatat cctgagtcgg gcgcggcagt gaaatactgt gcgtggggtg agaggcgaac
     1561 attcccggct gacgaatcgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg
     1621 gcggttaaca ggatcagttt ttctccggtt aacccagctt caatcagtgc cggatagaga
     1681 tttgctaccg ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc
     1741 agccccagcg ccaaatgccc cagtccacgc tcatcgaaat gggtgtggtg aatgaatgcg
     1801 gaattctccg ccgccgctaa cgttaacggt gtagaacgcg agccaggggc gatacagatg
     1861 tgtctgacgc cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat
     1921 gcgcttactg acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa
     1981 aaatagatta ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa
     2041 agtacgcagc cctgccgctt tgttatcgat ttcctgccac tcttgctcgg ggtcagaacc
     2101 acggacaatg cccgcgccag catatagccg cacgacattg ccactgattt ttgctgagcg
     2161 cagggaaacg cagaattcgc tttgttgtaa agagagatag cccgcagaac cggcgtacca
     2221 ttctcgggtg aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc
     2281 agcaactgct gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag
     2341 tgcagtccag atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg
     2401 taaaacatcc aacgtctggg tatcggcctg caatcgttgg cagatatcct ccaccaccaa
     2461 catgttctcg cgctggtttt tatcatccgc catcagccac tcacctaact gctgcgcctg
     2521 cttatcatca ggatgatttg ctaccgttcc cgccagcgct tcagtacgca gcgctttgtc
     2581 acgccgccgc cataaccgtt ccggtgaaga gccaagaaaa gcaatttcgc catcaaaggc
     2641 catgtaaaaa tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc
     2701 attgaccgga cttgcgaaat gcaggtcagt tgcccgagca agcaccactt tgtcgagctc
     2761 accttcggcg atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg
     2821 ccagtgttgt tctcgggtgg tggttaaatg taacccaggc aagggtttaa tactcaccag
     2881 tgtggcgata aattcttttg ccttaatcgc atcatgctga agggagcttt cgctgaatag
     2941 cgtcagccgc agcgtggctt taccgccaca gcgtcgccat tcgaggcgtg gtaaaagtaa
     3001 attgccctgc gacgggtcaa acgcatttag cccccagatg cgtaagtcgg cgtgttccgg
     3061 gtgctgacga agaaacgttt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag
     3121 gacggcagct tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg
     3181 actggccagc cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcaataac
     3241 ccgaatgccg ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc
     3301 cgtagtaagt gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac
     3361 gacaaaaaaa atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc
     3421 tagtaccagc ccaacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta
     3481 gtcatcagca cgataaacag cctgcttcgc ccgatagtaa taactgtctg aagcctggct
     3541 aacccgtttt ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt
     3601 ctgatcggcg ggatcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt
     3661 caggtcatca tcaatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgtg
     3721 taaaacctgt cactaactat agacaagggt tattgattac gcctgaatta cctcgcgtgc
     3781 catcccaatg tgtgggatac catcttcttc ataaatatcg gtcacaggga tgaaaccaaa
     3841 ttgcccataa aagttttgta aatgcgcctg cgcccccaga taaatcggct tctccggcca
     3901 gcggcgagta cacgattcca gcgccttact catcaattgc tgaccgattt tttcaccgcg
     3961 taaggcttcg ctgacaatca cccgaccaat cacaacgggt tgcagttcgt cgtcactttt
     4021 cagaatcctc gcatacgcca ccagtgtccc gttatgccaa ccgagaatgt ggcgattttc
     4081 tccctccaga tcgtcaccat cgatatcctg gtaaggacag ttttgttcca ctacaaatac
     4141 cgcacaacgc agttgtaata aggcataaag ctgagaaacg gataaatcag aatgatgcag
     4201 atcttgccag tcgatcatgg aaggctcctt tgtgctgttt gctttatact agccggaata
     4261 ttttcagcag cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc
     4321 aacccgtacg cgcaatgtca cggcaatatt acttaacctg caacatccga cccagagcgg
     4381 tctttggttg tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa
     4441 ccctggaaaa ctggacaaga tttttatcag tcaccttcat ggcgatcatc tctttggttt
     4501 acccggcttg ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg
     4561 tccgcacggt atccgtgaat ttgtggagac cgcgctgcgg attagcggct catggaccga
     4621 ttatccgctg gaaattgtcg aaattggcgc tggcgaaatt ttcgatgatg gcctgcgcaa
     4681 agtgaccgct tatccgctgg aacacccact ggaatgctat ggctatcgta ttgaagaaca
     4741 tgataaaccg ggtgcattaa atgctcaggc attaaaagct gctggcgtgc cgcctggccc
     4801 actgtttcag gcattaaaag cgggcaaaac aatcacgctg gaagacggaa ggcagattaa
     4861 cggcgcagat tacttagccg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac
     4921 cggcccctgc aatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc
     4981 gacgctggat ataaccatgg aagccaaagc caatagtcgc ggtcatagct ctacacgcca
     5041 ggctgcgaca ctggcccgtg aggctggagt cggtaagcta atcattaccc acgtcagctc
     5101 gcgctatgat gacaaagggt gtcagcacct gttacgtgaa tgcaggtcaa ttttcccggt
     5161 gactgaacta gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac
     5221 agggcgaaac ttgccctgtt atcgcaaccc gcgctacata tatccaccca cacgggtata
     5281 ttgagcgatt tttgatattt attcatctcc tcacgcacta tattcagagc aataagttgc
     5341 ctctttttat cttcattcag cacaaccttt gcaactaaaa ggagcctatg atggttacag
     5401 ttgtcagcaa ttattgtcaa ttatctcaaa cccaactctg tcagacattt gcagaaaaat
     5461 ttactgtgac cgaggaatta ctgcagtctt taaaaaaaca cttataactc attacaaata
     5521 atgatgaaat aacgcttttt attgataatt tacatatgaa aaaaatgaca tctctttgtt
     5581 ttataccatt actactatcg cttactttta ttatttttta ccaaccctgg gtcagggcat
     5641 taccaccaac gccccgacac gctagtcctg agcaattaga aaaaacagtt cgttatctta
     5701 cacaaactgt gcatccacgt agcgccgaca atattgataa tctgaatagg tccgcagagt
     5761 atataaaaga ggtctttatc agtagcggtg ccagagttac cgcgcaggat gtccccatta
     5821 cgggtggccc ctacaaaaac attgttgctg attatggtcc tgccgatgga ccgctgatta
     5881 ttattggtgc gcactatgac tctgtcagta gttatgaaaa cgatcaattg acctatacgc
     5941 cgggcgcgga tgataacgcc agcggcgtgg caggattact cgaactggcg cgtttgttac
     6001 agctgcagac tccagggatt ggcgtccaac tggtcgccta tgcgtcggaa gaaccgccct
     6061 tctttcgtag cgatgaaatg gggagcgcgg tacatgcagc ttcgcttgag cgtccagtga
     6121 aattaatgat agcgctggag atgattggtt attacgactc aacgcctggt agccaggatt
     6181 acccatcccc ggcaatgtcc tggctttatc ccgatcgggg agactttatt gccgtggtcg
     6241 gcagaatgca ggatatcaac gccgttcgtc aggtaaaagc ggcattgttg tcatcccggg
     6301 atttatctgt ttattctatg aatgccccag gttttattcc cggtattgat ttctctgatc
     6361 accttaatta ttggcaacac gatattcccg ccgtaatgat tactgacacc gctttttatc
     6421 gtaataaaca ataccacttg cccggtgata tcgcagacag attaaattat cagaaaatgg
     6481 ctcaggtagt agatgga
//
LOCUS       DAZGAP010000236         6482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGAP010000236 DAZGAP010000000
VERSION     DAZGAP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.237"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..995)
                     /gene="ydeQ"
                     /locus_tag="ACHLAM_004671"
     CDS             complement(81..995)
                     /gene="ydeQ"
                     /locus_tag="ACHLAM_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7856090.1"
                     /translation="MDKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGVAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(1055..1558)
                     /gene="ydeR"
                     /locus_tag="ACHLAM_004672"
     CDS             complement(1055..1558)
                     /gene="ydeR"
                     /locus_tag="ACHLAM_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7856091.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(1571..2101)
                     /gene="ydeS"
                     /locus_tag="ACHLAM_004673"
     CDS             complement(1571..2101)
                     /gene="ydeS"
                     /locus_tag="ACHLAM_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7856092.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(2115..4766)
                     /locus_tag="ACHLAM_004674"
     CDS             complement(2115..4766)
                     /locus_tag="ACHLAM_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7856093.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGHEAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKDADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEEYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPAVSPETLLNQQTAICR"
     gene            complement(4808..5518)
                     /locus_tag="ACHLAM_004675"
     CDS             complement(4808..5518)
                     /locus_tag="ACHLAM_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7856094.1"
                     /translation="MQTTRPPYSVSFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAASGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(5881..6444)
                     /gene="fimA"
                     /locus_tag="ACHLAM_004676"
     CDS             complement(5881..6444)
                     /gene="fimA"
                     /locus_tag="ACHLAM_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7856095.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         1795 a         1446 c         1389 g         1852 t
ORIGIN      
        1 tgtaggataa tagccttcac aaaataatta tccgcgcaat tttcgcgaat ggagcgcata
       61 gactaaggtc aaaatcacaa ttattcatag ataaaagtga caccaatgac tgactgaact
      121 gttccggctg aaactttgtt tcctgtttgt ccataggttg cgcttaaacc cagaggtact
      181 ttgcttttat ttactgtccc cagggagacg ttttctcccg tagccagtat ttttccatta
      241 cgcatcagag agacacccac tcccgatgct tttgttgcat ctggtgctgt gttagcaaac
      301 acttgtctgg cactgtcagt ggtagcaccc gaaagataaa atgagagctt ttgttcactt
      361 gagcaatata cgccaagggg tatttctgcg cttccaggaa agtccgggag attcacgttg
      421 acattacgac tatcaacggt gcagccgcct gttggcataa caacactgtt atttgaaata
      481 atattccacg taaaattacg cggatttccg cttcctaacg tggcaatttt atacatatga
      541 atacgggcaa taacttcccc tgcttttatg acaactccac cggccactcc gactggggta
      601 atatagagtt ttaacggtaa tggcatcggt gtcttatcac caatatccaa tacgttggta
      661 tttgtcgtca acgggaaggg gtaggtcaca ttattccagt acagtgatcc tttatatgac
      721 tgtagggagc ctgcgaaagc tgatccttgt accaggttta tatgatcagt gtcgtaccag
      781 ccgccgtaat cattccagca acttatatgc tgagacaagt ctacaaccag attctggccc
      841 ggttgtatta caggatcgag attaacataa acagaggttg tacctgctcc aatacttgac
      901 ccgccatcga cattacaact gaaggcaaaa actttgcctg ccattaatag aagataaata
      961 ccgaacagga cctttatact gaatgtttta tccatgatat atcctaaggt taaaaaattg
     1021 atttaaaaag gaggcaaaaa aaatcaaatt gatgttattg ccaggtatag atcacattga
     1081 ttagcgcctc gatcgttccc tggcttgcgt ttccattcac cgtgatagct cttgccttaa
     1141 gtggaaactg tgcattacga gtgatctcat caacaataac cgttttgcta tcgccatttt
     1201 ttaacgtcgc atcctggtca tcccttagct ctatctgaat attttcggca gtaccttcat
     1261 ttttgtaata acccgtattg tcagttgaac ctgtcacgat tgccgtcact gcacttgttt
     1321 caaccggaca atcggttaat gacaaagtaa tattgtgcca gcctgatgca gaaccaggtt
     1381 gttgcagatt gcgcgtataa agatccccga gattaacgtt agcttctttg gtttgaatag
     1441 tgcagggttt agcgactacc cgaccattaa cagtgatagt gacatcggcg gcctggagag
     1501 ttgctgtgaa taacgcgcaa aacgtcgcta acaggaacct cttgtgaagt cttttcatat
     1561 ctggctctga ttattgaaat tcaagggtaa aggttgccga agccctcacc agtcccggat
     1621 tgacggactt ctgagttgac ttcagacgag cggagtaagg caaaatattg ttctgttctg
     1681 gtaccagtgg gatccactgc atcccggcat gaaggtcatt caatttcacc ggacgcatat
     1741 ttccgtccag tatttctata cccaaaccgg aggccgtgtt gcttccttca tccagtttca
     1801 acagagtatt attttctgca tcctcaatac cgttaaatgc aacccgaacc cccgttgtcc
     1861 ctttactgca ttcacttaac gtaatctgaa aagggacggc tggacttgtg ctaccggtcg
     1921 ttggaaattg tctggcactg tttttttgga gatctacggt aaaattaagc gaatccgatg
     1981 agactgtgca gccataatcg agaacgcgcc cgctaatttt aataacgcta tctgcagata
     2041 aagcagaata ggtggttaac cccagacata aaccgaggaa aataacgtta ttgtatttca
     2101 taatctattg ttccttagcg acagattgct gtctgctggt tcagcaaggt ttcaggagag
     2161 actgcaggaa gcttgtactc gacaatacag tttgagtttt tatctttgcc ccatgaaacc
     2221 tgtaatttcc ctgactgtgg aagtccagtc agataaacct gaccgttttc cgcgacaatg
     2281 ctgccatttt tattctcccc atgtgtgaca attgcaccga atggaacgct cttattaccg
     2341 tacttcaacg tcattaatac tttcccgccg atttgtgcat taaatgttgc tctggcaata
     2401 gcaccgtgag ttgggatgac agtgacaacg gtttcatcca gttcaacatt atctgcaagg
     2461 gaattcgcgt taagagcgac acgattttct ctatattctg tcgcaaatgg taatatggca
     2521 tagccacgcc agtcggtatg aattccggtc tggttctcta ttttgacatt atcagcgcca
     2581 ggagccttaa ccagaaccat tgtgtcgccc agcggctgtc caaaggtgat gccatcagca
     2641 tgagcaataa tgccaccact cattccgtaa taaatctggc tgctgtcacc actccgactg
     2701 taaccgacat tcgtattacc gtaagctcca cgataattaa gagaactgta accactggtg
     2761 ccagacgatg tattaccccc ttgggtgtta ccgacctgaa cgctataatt caggttatta
     2821 tccggcagca gagtgccata aacccctgat aggttggtca tgccgccttt caaatcgttt
     2881 gacatactgt aactggcgtt tgaattacga aatgccgact gactgtctgt acgcatccaa
     2941 tgactgaagg gaacattaag cgtaaaagca agtaaatgat cccgatcgtt ttgccatata
     3001 ttattggaat agctgtaatt cagcgaagtc gtaatatcac caaacggcac atttaatcca
     3061 aatgatattt gctggtcgct gcgtgacgtg ttccagtaac tttggcgact ggcactgaaa
     3121 aatgtcgtac cgtaatgtcc aagctgctga gagatgctta tttgttcctg accacgctta
     3181 ctgtaaaaca gattaaaata atcaataaat agtgtctgct cattgctgtc tccggtagga
     3241 ggcttgacgg tgtaaccact cattcgactg taggcactgt cggataagtt ataaaaacct
     3301 tgcgtagaat agcgatatcc tgcgacctgg atattggttc ccgtctggta gaaggatttg
     3361 ctgtagacgg atttaaccga ttgtccgctg tgacgggtgt catctgcaag cgtcgtattg
     3421 gcctgcgtga tatcgaaaga aaaggcacca aaaagtccta aatctttacc aataccaagg
     3481 ttgaaggcct gatattcttc tgcaatttgc attccgccat aaggtgtcca gtttccttcc
     3541 agtccatgca tcaagctacc ttgtatgaat ttgggggagc tttgcaggtt atttccacta
     3601 cgatattccc ccatggcaag cgcataacgc gtatatccag cacgttggag aaccggaaca
     3661 gacgaataag gaacatataa tgtctgaata ctgccgtctg catcttttat ggttacttgc
     3721 agatcaccac cattggccgc agagttgata tcatcaatag taaaagggcc gggtgggaca
     3781 gtagtctgat aaacatcgta tccattctgt tttacactca cttgtgcggt gccgcgggca
     3841 ataccatgaa tcaccggagc gaaaccatgt tggctatcgg gcaacatcgc ttctgttgaa
     3901 tttattttga ggccacgaaa gtttacacta tcaaaaatat cgccatcggt ataactatca
     3961 cctaccgtta agcgtgagcg taagggaata atgtcacgtt cagccgacgt attgatatgc
     4021 tgccatttat tgctgtcaga tgaattgcta ctcccactgt tataactcca cgttgagtta
     4081 tcgcgcagtc gccaactacc aatgttgatg ccactctgta aattcaaata tgcatagttg
     4141 gattttcctg cattatggtt actacgatta ttaatagaat taccattaaa actatagttt
     4201 agtagcccag catttattcc ttcttcccac agatccggag aaacatagcc gcgagccatt
     4261 ctgccaacat aaatttgcgg aacggagagt gatagatgtt gctgaccaac attaaactca
     4321 gatgatgcgt cagccaaacg atcccgaagt ggtgtgcatg cgctgttgtc cgacacatgt
     4381 ttttctgccg aatgttcctt attgtctaat aatgcagatt tttttatgcc aagactgaca
     4441 agaagatctg tactcaggca tgggatgaga tctgcgttat tatcatctgc aataaaagta
     4501 atatcccgac tagtcataaa ttcgtcgttc aaataaatgt cgacccgata tgtaccaggt
     4561 ggtgcctcgt gtcccttagt aaaggcggat aaatcgacag attcagcaag atcattgctt
     4621 aaaaaccgtg gattaaaata atactctgcg tcaacgggaa aaaccgcagt tgctaagcca
     4681 agggcaatgg taagccgtgc aaagcgaggc agtagtaaca cctgatgcat acgatatgcc
     4741 ttaaatgcgg cacgaaaggc tgtcattatt aattccgatt gagtttatta ttaattaagt
     4801 cactgcttta ctgcatggtt gccttaatac gcggagttaa tgcaccgtaa tcattgatcg
     4861 tctgaaaact gatatctccg gatgcggcat gcgggatatc caccgatact tcaccttttg
     4921 gcggcaccat ggtgtttggg agattgctat ttcctgcttt catattcgtt acggtaatga
     4981 agtagggcgt tggattaata agagtaagtt tgctaccgct gcgacgaaac cgaagcattg
     5041 caggcgcttc ttcaggtgcc attgccaggt tcgtggggcg ataaaacatt tttatgcggc
     5101 taataattgc cagctgtaat gtattctcat tcttctggtc tttttccata gcaggaatag
     5161 ctttaacatt cacccagaac aggctttcac ggtcgcctgg taactgatga ttagtggcgt
     5221 tgattatccg cagcgtgttc tcttttttac cttgcatgga aaaaagcggt ggcgtaatga
     5281 caaactgggt gtctttttgg tcacccgcat tttctatcca tgactgaatg agatagacac
     5341 tggcaggatc attattcgtg acaggcaata aaacctgttt ctggtttgcc ggataaatta
     5401 cccgcgtcgc acccagtgcg acagctgcgt tggcaacggt gctatggcaa gcaaagagca
     5461 ataaaagtaa cactgtcgcc ataaaggaaa cagagtaggg gggacgcgta gtttgcatct
     5521 gatgcattct ccatgtgtta agaggataag cggggaaacc gttacatcag acaatcggta
     5581 aatgttgcta atacattggc gagtcgccgg gtttccagac ttaatgcgtt tgaaataaat
     5641 gttggttctc ttttaatagg aaaaccttgc tataaagtaa atattttaaa taacagtaag
     5701 tttaactact gttgtttatt gaacgtttag atattatgtg attttttcat accgctccaa
     5761 aacacaaact attcctcatt aataatgaga tgaacattgg tgaatttctc gggtgtgcgt
     5821 agccatgcca cacacaccct gtttcaattt cagatgacgt atagccatct taagattgaa
     5881 ttatagatat tgaactttga aggtcgcatc agcgtttgca gtaccagcta ctgactgccc
     5941 gagagcaata taacgagcct ggaacggcaa tatattcgtg ccatcaatca agtcagtttt
     6001 agcgctaaaa gtggcaccat caagtattaa tacctccccc gtacggtcca aaatttgaat
     6061 accgacattt tgggcagagc ctgccgctga actctgcagc gctaacgtat cgtcattact
     6121 ggtgacagta gtacccagga atgcaacagc tgcattactg gaaacattgg tatcacaatc
     6181 attcagcttg atattgaagc caacggcgga gctgaggtca cccgctttag ccagacgaga
     6241 agaacggacc tgacccagct caaccgtttg gttcattgat tctgaatcaa tcgcacaagc
     6301 ggcatttacc acttcacctt taaaatgaat aacaccgcca ttaacagtgg tcgttactga
     6361 ttcatcaccc tcggctgcgc ttaccgcagc agacgacatc gccaatacag aaacgacaat
     6421 cataccgaca tgtttgagtt tcataattct ccaaaattta tatttaagta gctgaataaa
     6481 ga
//
LOCUS       DAZGAP010000237         6469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGAP010000237 DAZGAP010000000
VERSION     DAZGAP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.238"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..2255
                     /locus_tag="ACHLAM_004677"
     CDS             3..2255
                     /locus_tag="ACHLAM_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO7856096.1"
                     /translation="MKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGK
                     TTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGFG
                     RVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGINA
                     LAGSVNMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGV
                     MAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSARTY
                     ENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQNR
                     SDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAPS
                     GQQDISALYTGLKVTRGIWEADFNLNYTRNRITGHKPACDSRVICVPQGSYDIDDKVG
                     GFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQA
                     GFNIDTRDLVVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDWEGI
                     SDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYFGA
                     GDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDLP
                     QIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARGR
                     TWIFGGDIRF"
     gene            2324..3499
                     /gene="senB"
                     /locus_tag="ACHLAM_004678"
     CDS             2324..3499
                     /gene="senB"
                     /locus_tag="ACHLAM_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFO7856097.1"
                     /translation="MNIFTLSKTPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSGDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(4129..4587)
                     /locus_tag="ACHLAM_004679"
                     /pseudo
     CDS             complement(4129..4587)
                     /locus_tag="ACHLAM_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(5027..5413)
                     /locus_tag="ACHLAM_004680"
     CDS             complement(5027..5413)
                     /locus_tag="ACHLAM_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO7856098.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGIHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(5410..5634)
                     /locus_tag="ACHLAM_004681"
     CDS             complement(5410..5634)
                     /locus_tag="ACHLAM_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7856099.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            5700..6055
                     /locus_tag="ACHLAM_004682"
                     /pseudo
     CDS             5700..6055
                     /locus_tag="ACHLAM_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6288..6419)
                     /locus_tag="ACHLAM_004683"
                     /pseudo
     CDS             complement(6288..6419)
                     /locus_tag="ACHLAM_004683"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
BASE COUNT         1836 a         1515 c         1481 g         1637 t
ORIGIN      
        1 ttataaaact ggctatcggc tcaggcatat tattgctcag ctgcggtgct tactcacaat
       61 ccatcagtga aaaaactaat tccgacaaaa aaggagcggc agaattcagt ccgctcagcg
      121 tttctgtcgg gaagacgacc agtgagcagg aagctctcga gaaaacaggc gcgaccagtt
      181 cccggacaac ggacaaaaac ctgcaatcac ttgacgcaac agtgcgtagt atgcccggta
      241 cttatactca aatagatcct ggtcagggag caatcagtgt gaatattcga ggcatgagcg
      301 gatttggtcg tgtaaacact atggtcgatg gtattaccca gagtttttac ggaacctcta
      361 cctccggaac aacgacgcat ggttcaacta acaatatggc tggcgtactt atagatccta
      421 acttactggt agcagttgat gttacacgcg gtgacagcag tggctctgaa gggatcaacg
      481 cccttgccgg tagtgtaaat atgcgtacta ttggcgttga cgatgtaata tttaacggta
      541 atacatatgg ccttcgttca cgtttctctg tcggtagtaa tgggctggga cgcagcggaa
      601 tgatcgccct tggtggaaaa agcgacgctt ttacggatac gggaagcatt ggcgttatgg
      661 ctgctgtgag cggcagttct gtgtactcta atttctcaaa tggttctgga attaacagca
      721 aagagtttgg ttatgataaa tatatgaagc agaaccccaa atcccaactg tataaaatgg
      781 atatcagacc agacgaattt aacagcttcg aactttccgc tcgaacctat gaaaataaat
      841 ttacacgtcg tgatataacc agtgacgact attacattaa atatcattac accccttttt
      901 ctgaattaat tgactttaac gtaacggcca gtaccagtcg cggtaatcaa aagtatcgtg
      961 atggctcgct gtatactttc tacaaaacct cagcgcaaaa tcgttctgac gcgctggata
     1021 tcaacaatac cagccggttc actgtcgcgg acaatgacct ggagtttatg ctgggcagca
     1081 aactgatgcg tacccgctat gaccggacca ttcactcagc ggcgggcgac ccgaaagcga
     1141 atcaggaatc gatcgagaac aatccgttcg caccctccgg ccagcaggat atttcagcgc
     1201 tgtataccgg gctgaaggtt acgcgcggca tctgggaggc agatttcaat ctcaactaca
     1261 cacgtaacag gatcacaggg cacaagcccg cctgcgattc acgcgttatc tgcgtgccac
     1321 agggtagcta cgatattgac gataaagtgg gtggcttcaa cccttcagtt cagctttctg
     1381 ctcaggtaac accatggctt cagccgttca ttggctacag caaatccatg cgcgccccga
     1441 acatccagga gatgttcttc tctaattcag gaggcgcatc catgaaccca ttcctgaagc
     1501 ctgaacgtgc agaaacctgg caggcgggtt ttaacattga taccagagat ttagtggtcg
     1561 aacaggatgc cctgcgcttt aaggctctgg cgtaccgcag caggatccag aactacatct
     1621 acagcgagtc ttatctggtt tgttctggag gtcgtaaatg cagtatggct gaggtgattg
     1681 gcaatgactg ggagggcatt agcgatgaat acagcgacaa tatgtacatc tacgttaact
     1741 cggcaagcga cgttatcgca aagggcttcg aactggagat ggattatgat gcaggttttg
     1801 cttttggccg actctctttc agccagcagc aaacagacca gccaacctcc atcgccagca
     1861 cctactttgg cgcaggggat atgaccgaac tgcccagaaa atacatgacg ctggatactg
     1921 gtgttcgctt cttcgataac gcgttgaccc tgggcactat cataaaatac acaggcaagg
     1981 ctcgtcgcct gtcgcctgat tttgagcagg acgaacatac cggcgcaata atcaaacagg
     2041 atttgccgca gatcccaacg attatcgatc tctatggtac ttacgagtac aaccgcaacc
     2101 tgacactgaa actttcggta caaaacctga tgaacagaga ttattcggag gcgctgaata
     2161 agctcaacat gatgccaggt cttggtgacg agacccaccc agccaattcc gcgcgtggca
     2221 gaacatggat atttggcggg gacattcgtt tctgatgaca atttccgcta ggttcagaca
     2281 atacgttttc tctcttatgt caattttatt gcaggaacga aaaatgaata ttttcacttt
     2341 atccaaaaca ccgctatacc tgttaatttc actattttta cccacgatgg ccatggctat
     2401 cgatccacct gaacgcgaac tttcgcgatt tgccctgaaa acgaattacc ttcagtcccc
     2461 tgatgaaggc gtctatgaac tggcgtttga taatgccagt aaaaaggtgt ttgcagcagt
     2521 caccgatcgt gtaaatcgtg aagccaataa aggctatctg tattcgttta attcagattc
     2581 gctgaaagtc gaaaataaat acacgatgcc ataccgggca ttttcgctgg cgataaatca
     2641 ggataaacat cagctctata tcggacacac ccagtcagcg tccctgcgta tcagtatgtt
     2701 tgacacccca accggcaaac tggtaagaac cagcgacagg ttaagtttta aagcggcaaa
     2761 cgctgcagat tcgcgttttg agcatttgcg ccatatggtt tacagccagg attccgatac
     2821 cctgtttgtg agttatagca atatgctgaa aacggccgag ggcatgaagc ctctgcataa
     2881 gctgttaatg ctcgacggga cgacgcttgc cttaaaaggc gaggttaagg atgcttacaa
     2941 aggtacagcg tatggtctga cgatggatga aaaaacacag aaaatctacg ttggcggaag
     3001 agattacatc aacgaaattg atgcgaaaaa tcagacgctg ctgcgtacca tcccgttgaa
     3061 agatccgaga ccacaaatca caagtgtgca gaatctggcg gtggactccg cttctgaccg
     3121 tgcctttgtg gtggtattcg accatgacga tcgttccggt acaaaagatg gactctatat
     3181 ttttgactta cgcgacggta aacagcttgg ctatgtgcac acaggagccg gagctaacgc
     3241 ggtgaaatac aatccgaaat ataacgaact gtatgtcacc aacttcacta gcggcaccat
     3301 cagcgtagtg gatgccacca aatacagcat cacccgtgaa tttaacatgc cggtctaccc
     3361 aaaccagatg gtgttgtcgg gtgatatgga taccctttac attggcatca aagaaggctt
     3421 taaccgcgat tgggatcctg atgtgtttgt ggaaggagct aaagaacgta ttctgagcat
     3481 tgatttgaaa aagtcgtgat cgggggaaaa catgggataa aaactccgtt attccactaa
     3541 actggccgga ctaggaaaag caaacatctc tgtcctgccg gtacgcctgt tacgccgctc
     3601 catgaggctg aggaatctcc agaaaacgag acaggcacag cattgtacga tcaactgatt
     3661 tgtgccaata attcaatgaa atcacagcta tgcctgttca tggggttcta ggccggaacc
     3721 ccagattttt ccgattgttt gttttctaac tgattattca gtagttttag agggctgacg
     3781 ctcagtggaa cgaaaactca cgttaagagg ttttgactat gataaaagtc aaagtagggg
     3841 tgagtttgga tatggaaatt attgtactat aagggcttcc ccgaacagtc aacgtcggta
     3901 tttatggttt gtctgcacta cagttaaaaa taaaggggag agactgcggt actataaacg
     3961 gtcataataa atcgttatcg attcggaccc tgatgaaaga cgcgcgtgag gctcttgttc
     4021 gttaagcgga tacgtatatc ctgtacaata atcagatata cctcacgggg tctaaccctt
     4081 tatcatcaac gagttgcagg tcagatagtc atacggttgc tgcgtgcttt agctggctga
     4141 atacagtctg aaatcatcac cgtaatgcaa tgatgcccag cacagccttg catttttggc
     4201 ggcgatggcc acaacagcgc gccagtatcc tctgcgttcg accagtgaac agacccaacg
     4261 actgaatgag tcagtccttt tctcagcgcc aatcagtact gaacgagcac cctgaaccag
     4321 aagcgttcgc agatacgaat caccggcttt tgttatcctg cctagcttcg actttccgcc
     4381 gctgctgtat tgtgatggag tcagtcccag ccaggccgcc agttgccgtc catttttaaa
     4441 atcatgtgca ttaccgatac aggcaaccag cgcacaggca gttgtgggac caattccttt
     4501 tagctccatc agccgctgac tgcggtgatc tgttcttgct atacgggaca aaattttgtc
     4561 atattcagtg atgttagctt caatgcgggg tcagtttgga gatcgtaaaa ttttgttcgt
     4621 taagcacatt atttaaccaa tagatatgcg taacggattg attttgcgtc aacggctatc
     4681 gcttttaagt tatcgccatt gaggtattgc atttaggtta tccgatagag gtcaaatcct
     4741 ctgaaaacgc cgtatagcac gggagtacac cctaccgcaa tagcgcaacc gtacaatatt
     4801 gtacgtttcc caaacgcagc ctaggaagca cgtcgtcagt attggagaaa acttacaaac
     4861 gctcgccgca gccccagcga ccgaacgtta gtgagcgagt gggcgaggaa gcggaaaagt
     4921 gtcaggtctg aattgagcac atacgccaga aattatatag caaaaagata tatgattttt
     4981 acattgctca gtagattgta aaatgctgct gattggatat ctatatctaa tttaaaataa
     5041 tggctttaag atggtcagaa accatcttcc agttatattc tcctgttgca gcattcaaag
     5101 cgataatttg catttcatca aacgaaatac gcgaactaat aatatgtata ccattccgtt
     5161 ttaaaaaaac ctgtgcaaca aacaaggctg ttcgcttatt tgcatcgttg aaaatgtgac
     5221 cacgagcaat agcaactaaa tacacggcag ccagatccat aatatctgtc acaccttcat
     5281 aatgatacat gttcaaaaca cgatgaatga tcgcgtcagc gcgaccgggt tctggcatac
     5341 ccgccacgcc accatcacgc gaaatcaagc ggtcatgaaa ctcaatcatt tcctgtgaac
     5401 taacgaattt catcgcttag aaagctccgt ataggccgtc ccatgacttt gcatgatctc
     5461 agcaaactca gcatcaagag ccgctttacg gtatcgttca aaagcgtctt tatcaaccag
     5521 tacagccgcg ccacctttac ggcgctgaat ctcaacaggc tgattttcat caaccgccag
     5581 ctcgagcaac gaggctaggt tatcgcgagc atggctatag ttacatgtaa acatagtgct
     5641 tcctctttga gttgtacaat taaattgtac aacagttatc ttcaatatga aatcaccgtt
     5701 accgctggcg catcctgacc caacttaacc ggggagaaag tcgccatgcc gttgccagag
     5761 ctatctgtca cggtcagaaa ggtgagataa gaaagcgata taccgatggt caagaagatc
     5821 aactgggcgc actggggctg gtgactaacg ccgtcgtatt atggaacact atttatatgc
     5881 aggctgccct ggaccatttc agggcacagg gggaaatcgt gaatgatgaa gatatcgcac
     5941 gcctctcacc actttgccac ggacacatca atatgctcgg ccatattcct tcacgctggc
     6001 agaactggtg actaaagggc aaaaagaggc atcggaggaa gaaaacattg cttaacgtga
     6061 gttttcgttc cactgagcgc cagcccccta attctctata tccaaactga ccccaatagg
     6121 tgacgggtta tgtgacgaaa tctgatgtag aaatcgttgt ttcagtgaca gtcactcaat
     6181 cggtcattta tgtgacaccc cacgccgtta ctggtcgcgg aaaaatccag cggaaaaatc
     6241 caggtttccg gcttgggacc ccatctcaag agatcctttg atcttttcta ctgaaccgcg
     6301 atccccgtca gttcagaaga cgaagatgac gcaacggttc ctcattgtac atcacgaata
     6361 tagtttgctt gacatcctga caagaataat attttaatta gaaaaattat tattttcata
     6421 tccctttccg acaaaagtaa aaatacttat gcatttaaaa tactaccta
//
LOCUS       DAZGAP010000238         6449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGAP010000238 DAZGAP010000000
VERSION     DAZGAP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.239"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..821
                     /locus_tag="ACHLAM_004684"
     CDS             <1..821
                     /locus_tag="ACHLAM_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO7856100.1"
                     /translation="TVAVLVPSLTDKTSSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     AEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFE
                     ASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSC
                     DQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1024..1704
                     /locus_tag="ACHLAM_004685"
                     /pseudo
     CDS             1024..1704
                     /locus_tag="ACHLAM_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1721..2029
                     /locus_tag="ACHLAM_004686"
                     /pseudo
     CDS             1721..2029
                     /locus_tag="ACHLAM_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2132..3238)
                     /locus_tag="ACHLAM_004687"
                     /pseudo
     CDS             complement(2132..3238)
                     /locus_tag="ACHLAM_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4004..6052
                     /gene="ireA"
                     /locus_tag="ACHLAM_004688"
     CDS             4004..6052
                     /gene="ireA"
                     /locus_tag="ACHLAM_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO7856101.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
BASE COUNT         1835 a         1413 c         1459 g         1742 t
ORIGIN      
        1 atacagtcgc cgtactggtt ccttccctga cagataaaac cagctcccgc tttatgcagt
       61 cgttgcagca ggtgcttaat aaaaatgaat tccagctact gcttgggtgt catgagtata
      121 atcagctcaa agaagctgaa atcctgatga cgcttcttca aggtaacccg gcggcgttag
      181 ttatttttgg ttctcagttg gctgataaaa ctcaccagat actcgagaaa acaaatatac
      241 ctaccattaa cgttgtcggt tcccctttca gtccggctca aattaccatc gaaacagcat
      301 tttttgaagc atcacataaa ctgaccgaac atctgctgga acagggttat aagaatatcg
      361 gatttatcgg tgcgcatatg gacaaccgcc tgcaacgtca gcaactgaac ggctggcata
      421 aagcaatgct gagctattac cagaactctg atcttgttat caccatgccg ggtgccgcaa
      481 gtttgcaatt ggggcgttac gcattaaacg agatattact gcgacaacca gagctggatg
      541 ctgtgatttg cagccacgaa gagattgcgc tgggtatcat gtttgagtgc cagcggcgtt
      601 tgttaaaaat ccctggaaat atcgctgtag cttgtcttga cggttcagac agttgcgatc
      661 aaacacaccc aaccttgaca tccatccgta ttgactacaa aaaaatgggg acagagacgg
      721 gtaagctgct gattggtcta ttgaataata atcatgatga atcagaagag tcgcgtatcg
      781 tgcaattcaa ctaccagata gaattacgtc agagtacatg agctcaaaag gggattattg
      841 caaagttagc tattgtggta gggatagccg tttccggcta ttccccgcgc agagccggat
      901 gtgtgctgct aacgcatcct gtcaacgacg gatgaaaagt tatccactta tatctccacc
      961 aacggcccaa tattgtatcg ttcgtgacac atccatttct acttcaggta tttggcgtag
     1021 tagcgggtgc taaaaatttg ccgcaaaacc gcaatatcag cccgcagagg cgctctccag
     1081 attatccatt cctatattat ctctgcaccg gatgaattac tcgaacaact cagaaatatg
     1141 acacatatgc aacttatcag gactctggga tcctggaggc ctgatgccag tgaatacagt
     1201 aatgttacca acgtttatcg cattacattg aaatcccttg ttcgacgcta tttcgagttt
     1261 catgatgaaa tcaccaactt ggatgtcatg attgcggcaa ttgttgatga actggcacct
     1321 gaactgatta aacgtaatgc tgttggatac gaaagcgctt tgcagttgtt gatctctgcc
     1381 ggagacaatc cctaacggtt aagaaccgaa tcaggttttg cggcgttgcg cggtgtcagc
     1441 cagtttcccc tatcttctgg aaaaacgaac cattaccgac ttaatcgggg tggggagcgt
     1501 gttgcaaata gtgcacttta catcatcact ataggatgtt tacaaactga cgataaaaca
     1561 aaggaatatg tctccaggcg agtagccgaa ggacatacaa aaatggaagc aatacgctgt
     1621 ctgaagcgct atatctcacg cgaagtttat acattactac gaaatcaaaa caggcatgtt
     1681 aactgcatcc cgataacgtc ttgactctta gaagggcgtc caaggcagtc attattcgag
     1741 caggcagttc cggcagttat tgtgtcgata ccagatcagg cagagtatga gccggcgcgg
     1801 aaaatgctgg gataacagct caatggaacg cttcttcagg agtctgaaga acgaatggat
     1861 gccggtggtg ggttacgtaa gcttcagcga ggcagctcac gccataacgg acgatatcgt
     1921 tggatattac agcgcactaa gaccgcacga atataacagt gggttatccc caaacgaatc
     1981 ggaaaatcga tactggaaaa actctaactc ggtggccagt ttttgttgac cacttcagcc
     2041 atcgccagac gctaacatga gttactcttg ttctgggcta cgtcaccttc agggaactgg
     2101 ttatcctcat tctttatttg tgacattgtg gttattgtgc gacaaacatg gctatcccgc
     2161 cccatatccc agataaaacc acctgctcac gtgcgacagc gacaattgta atattgacat
     2221 tctttccttt ggcctgaagt tttcgagacc tcttgcaaag cctgagttga gcatcccatg
     2281 ctctgtcaat gacggggcgg ggtaaatttt cctgattttt tgtattgccg gactgatgcg
     2341 tgtcggatgc cggtaactcc acgctgcctc aaccaatagc tttctggcat agctgtttcc
     2401 ggctttggta atgctgccct gatgtctgct tccacccgat gaatattctg atggcgtgat
     2461 accaaaccat gacatcagtt gtcgtggatg ttcaaaacgg gtcagatcac ccagttcaga
     2521 aattaatcca accgcagtaa taaactgaat ccctcgcatg gcttgctgcg cctctactac
     2581 cggatacagc ctccattcgg taacggcctc cttcgggcgg attccagccg ttcacattgt
     2641 gcctgccggt cttctattgt acaacggtgt tcgtcaaaag ctaattgtcg ccagggactt
     2701 tcgaatgagt atttgctgag ccagtgtcgg tgtgcagggc cccaatctgc tcgcccgaca
     2761 taatggaccc catgaaccag aagaaacgat ttcagacgtt gccttgcatg ccgtaaatca
     2821 tcgcgggcag atgcccatgc ccgggccaaa tcccggaatg tctcatcttc aacgccggat
     2881 acgtagacag cagaaagatc tcctgcacgc agtgaacgca caagtctgat ggcatcacgg
     2941 tgtcggtttt accccgctcc cccggttttt tcgggataag tgagggggcg cagaccatgc
     3001 agtcaaaacc ggatttcacc agccgacgat aaagtccata accacagggt cccgctttat
     3061 aaacgatact gacctgcgat gactttgacc tgatacgttg ataaagatta tgaatgtcag
     3121 taggtgatgt gctaatttta cccatcagtt caacaggttc tgaattgata gcataggaga
     3181 cagtaatcga ttctttgtga acatctaatt cgacgtacaa catgttattg ttgttcatgc
     3241 tggcctccgt ggtggaaatt ccaggggggt agcaacagct cacccatgcg gctctgacaa
     3301 cgatgttaac ccgcattaaa tatcccacgt tggacagcat ctgtctcacg gaaagtcata
     3361 ctgccttacg tgcatatttc accttacgcc ttcactgtct gtcatcggaa gtactgattt
     3421 atattggggc tttgcactta ctgtcattca cgggaaaaga tggtggtgaa cagtacgata
     3481 ctgtcttggc tgttgccgag ggaagattca ggagtggtaa gggggttagt ggtgtaagat
     3541 gagtctggag ctctgactgg tcagtgacgc gaaactcaat ctgccagggt aaactccccc
     3601 ttgcttttgc gcagaagcag tgggccttgg ttcgcttatg tcctgcatct acaagcgaat
     3661 gactgaccgg agactcccat atctggtcag tcgcccattt ttattttcca ccacatcgga
     3721 caagcatacc gtaataaaca gcaccagtct cctgttcact cttgcttgat ttactggaat
     3781 gctgtttatg gagatatccg gttgaaagat tttacctctt cgacaggact ataaattata
     3841 cagtaagaag tccctcgtag aaaggggctg attctcatca agtcggacag gttcaaagtg
     3901 ttacctgaat gagctaaatg ttatttacct tcctttctgt tcattgtaaa tttttcttgc
     3961 taacgcaaat cattatcatt acgattttag aaaagggaaa tttatgaaga acaaatatat
     4021 cattgctccg ggcattgccg tgatatgttc tgcagtcata tcatcaggtt atgccagttc
     4081 tgataaaaaa gaagatacgc ttgttgttac tgcctccggc ttcactcagc aactcagaaa
     4141 tgcaccggcc agtgtttcag tcattacttc agagcaactg caaaaaaaac cggtttcaga
     4201 cctggtcgat gcggtaaaag atgttgaagg gattagtatc accggtggga atgaaaaacc
     4261 ggatatcagt atacgtgggt taagtggcga ttatacattg attctggtcg atggacgacg
     4321 tcagagtggt cgggaatcca gaccaaacgg cagcggcggt tttgaagccg gatttatccc
     4381 tcctgtggaa gcaattgagc gtattgaagt tatacgtggc cctatgtctt ccctgtatgg
     4441 ttccgatgct atcggaggag ttattaatat cataaccaaa cctgttaata accagacatg
     4501 ggatggcgta cttgggcttg ggggaattat tcaggaacat gggaaatttg gtaactcaac
     4561 cacgaatgac ttctacctgt caggcccatt ggttaaggat aaacttggtc ttcagttata
     4621 tggaggaatg aactatcgca aggaagatag tatctctcag ggaacaccag caaaagataa
     4681 taagaatata acggcaacac tccagtttac tccgactgaa agccagaagt ttgtttttga
     4741 atacggaaaa aataaccagg aacatacatt aacacctggt gattctcttg atgcctggac
     4801 tatgcgggga aatcttaaac aaccaaacag taaaagagaa acacataact cacgtagtca
     4861 ctgggtagca gcatggaatg cccagggcga aatactgcat cctgaaattg ctgtttatca
     4921 ggagaaagtt attcgtgagg ttaaatctgg taaaaaagat aaatataatc attgggatct
     4981 taattacgag tcaagaaaac cggaaataac caacacgatc atagatgcaa aagtgacggc
     5041 atttctgccg gaaaatgtgc tgaccatcgg aggtcaattt cagcatgcag aactccgtga
     5101 tgactcagcc acaggtaaaa aaacgacaga aacacagtct gtttcagtta aacagaaagc
     5161 tgtttttata gaaaatgaat atgctgcaac ggattctctc gccctgactg gagggctgcg
     5221 cctcgataac catgaaatct atggcagtta ctggaatcca agattgtatg ctgtttataa
     5281 cctgaccgat aatctcactc tcaaaggagg gatcgcaaaa gcatttcggg ccccttcgat
     5341 tcgtgaggtc agtcctggat ttggaacact gacgcagggt ggtgcctcta ttatgtatgg
     5401 aaacagggac ctgaaaccgg agaccagtgt aaccgaagag atcggtatta tttataataa
     5461 tgacagtggt ttttcagcaa gcgcgactct gtttaataca gattttaaaa ataagctgac
     5521 cagttacgat ataggcacaa aagatccagt cactgggcta aacactttta tttatgataa
     5581 tgtgggtgag gcaaatatca gaggtgtgga gcttgcaact cagattcctg tgtatgataa
     5641 atggcgtgta tctgcaaact atacattcac tgactcccgt cgagaaagcg atgacgaaag
     5701 tctcaatggc aagtcgctga aaggggaacc tctggaaaga actcccagac atgcagccaa
     5761 tgcaaaactg gaatgggatt acactcagga tattacattt tattcatctc tgaattatac
     5821 aggaaaacaa atctgggcag cacaaagaaa tggtgccaag gttccccgtg ttcgtaatgg
     5881 attcacttct atggatattg gtatgaatta ccagttcctg ccaggcacgc tgattaattt
     5941 tgccgtactt aacgtcacag acagaaagag cgaagatatc gataccattg atggtaactg
     6001 gcaggtcgat gaaggacgtc gttactgggc taatgtaaga atatccttct gagagaatat
     6061 agataacctg aggccgtgcg cctcaggtac cagtatagta atcagtaacg tcagcttcca
     6121 ggttgtaatg cttttaattt tgataaaaat aaacttaata aattacattt gttaaatatt
     6181 gataatttta atttggtttt attactccag ttatactcac actctatctt tacacaattc
     6241 atcaccatta cacctccata gcctgttgat ttaaaagatt taatttcctg tgaaacaatc
     6301 aggcctgtat gaacctgatt ataccacata atcttttaag ttatttaaat ttaactctcg
     6361 ggcttttctg tcagtaaaat cagaaagaat tgcaggattt cgtttttttg ctgcgaagtg
     6421 aaatgtatct catttttact gccttcgcc
//
LOCUS       DAZGAP010000239         6445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGAP010000239 DAZGAP010000000
VERSION     DAZGAP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.240"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..304)
                     /locus_tag="ACHLAM_004689"
     CDS             complement(<1..304)
                     /locus_tag="ACHLAM_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO7856102.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVT"
     gene            434..1606
                     /gene="torC"
                     /locus_tag="ACHLAM_004690"
     CDS             434..1606
                     /gene="torC"
                     /locus_tag="ACHLAM_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO7856103.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            1606..4152
                     /gene="torA"
                     /locus_tag="ACHLAM_004691"
     CDS             1606..4152
                     /gene="torA"
                     /locus_tag="ACHLAM_004691"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO7856104.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            4149..4748
                     /gene="torD"
                     /locus_tag="ACHLAM_004692"
     CDS             4149..4748
                     /gene="torD"
                     /locus_tag="ACHLAM_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO7856105.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATQTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(5003..5308)
                     /gene="cbpM"
                     /locus_tag="ACHLAM_004693"
     CDS             complement(5003..5308)
                     /gene="cbpM"
                     /locus_tag="ACHLAM_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO7856106.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(5308..6228)
                     /gene="cbpA"
                     /locus_tag="ACHLAM_004694"
     CDS             complement(5308..6228)
                     /gene="cbpA"
                     /locus_tag="ACHLAM_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO7856107.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         1538 a         1652 c         1776 g         1479 t
ORIGIN      
        1 tggtgacgta atcgtctgcg cccatttcca gcccaacaat acggtcaatc cgatcgctgc
       61 gtcccgtaac cagaataatc cccaccgttg agcgttctcg cagggcgcgg gttaacatca
      121 ggccattttc atcgggcaaa ttaatatcca gcagaattaa atccaccggc tgattctgca
      181 taatttcccg cagcccggca ccgctcgccg taacggaaac ggtatacccc tcctgagtga
      241 agtaggattg taatcgcgcc tgggtaaccg gctcatcttc aacaataaca atgtgatgtg
      301 gcatcagagg gttttactca ttctgttcat atctgttcat attctgccgt aagccgttca
      361 tcctgaccag tgccgctgtt catatttact cattaagatc gcttcactaa accataattt
      421 tacaggggct attatgcgga aactctggaa cgcgctacgc cgacccagtg ctcgttggtc
      481 ggtactggcg ctggtcgcta ttgggattgt gattggcatt gcgctgattg tattgccaca
      541 cgttgggatc aaagtcacca gcacaaccga attttgtgtc agttgccaca gtatgcaacc
      601 ggtgtatgaa gaatataaac agtcggtgca cttccagaac gcctccggcg tgcgagctga
      661 atgccatgac tgtcatatcc cgccggatat tccaggcatg gtgaagcgca aactggaagc
      721 gagcaacgac atttaccaga cctttattgc ccactccatt gatacacctg aaaaattcga
      781 agccaaacgc gcggaacttg ccgagcgtga atgggcgcga atgaaagaaa acaactcggc
      841 aacctgtcgc tcctgccata actacgatgc gatggatcat gccaagcagc atcctgaagc
      901 ggcgcgtcag atgaaggtgg cagcgaaaga taatcaatcc tgcatcgact gtcataaagg
      961 tattgcccac cagttaccgg atatgagtag tggcttccgt aagcagttcg atgagctgcg
     1021 cgccagtgct aatgatagtg gtgacacgct gtactccatt gatattaagc cgatttatgc
     1081 ggcgaaaggc gataaagaag cgtctggttc tctgctgcct gcttcggaag tgaaagtcct
     1141 taaacgtgac ggcgactggc tgcaaattga aatcactggt tggacggaaa gtgcaggacg
     1201 tcagcgtgta ctcacccaat tcccaggtaa acgcatcttt gttgcctcga ttcgtggtga
     1261 tgtgcagcag caggtgaaaa cgctggagaa aaccaccgtt gccgacacca ataccgagtg
     1321 gagcaagttg caggctactg cgtggatgaa gaaaggcgac atggtgaacg atatcaaacc
     1381 gatctgggct tatgcggatt cgctgtacaa cggcacctgt aaccagtgcc acggcgcacc
     1441 ggaaatctct cactttgacg ctaacggttg gatcggcacg ctcaacggca tgattggctt
     1501 taccagcctc gataaacgtg aagaacgcac cttgttgaaa tatctgcaaa tgaatgcgtc
     1561 tgacaccgca ggtaaggctc acggcgataa gaaggaagaa aaataatgaa caataacgat
     1621 ctctttcagg catcacgtcg gcgttttctg gcacaactcg gcggcttaac cgtcgccggg
     1681 atgctggggc cgtcattgtt aacgccgcgc cgtgcaactg cggcgcaagc ggcgactgag
     1741 gctgtcatct cgaaagaggg cattcttacc gggtcgcact ggggggctat ccgcgcgacg
     1801 gtgaaggagg gtcgcttcgt ggcagcaaaa ccgttcgaac tggataaata tccgtcgaaa
     1861 atgatcgccg gattgccgga tcatgtacac aacgcggcgc gtattcgtta tccgatggta
     1921 cgcgtggact ggctacgtaa gcgccatctg agcgacacct cccagcgcgg tgataaccgt
     1981 tttgtgcgcg tgagctggga tgaagccctc gacatgttct atgaagaact ggaacgcgta
     2041 cagaaaactc acgggccgag tgccttgctg accgccagtg gttggcaatc gacggggatg
     2101 ttccataacg cttcggggat gctggcgaaa gcgattgcct tgcatggtaa tagcgtgggt
     2161 acaggcggag attactctac cggggctgcg caggtgatcc tgccgcgcgt agtcggttcg
     2221 atggaagtgt atgaacagca aacctcctgg ccgctggtat tgcagaacag caaaaccatt
     2281 gtgctgtggg gctccgattt gctgaaaaac cagcaagcga actggtggtg cccggatcac
     2341 gatgtttatg aatattacga gcagctaaaa gcgaaagtcg ccgccggtga aattgaggtc
     2401 atcagcatcg atccggttgt cacatccacc catgagtatc tggggcgcga gcatgtgaag
     2461 cacattgcgg ttaacccgca aactgacgtg ccgctgcaac tggctctggc gcatacgctg
     2521 tacagtgaaa acctgtacga caaaaacttc ctcgctaact actgtgtagg ttttgagcag
     2581 ttcctgccgt atctgctggg tgagaaagac ggtcagccga aagatgccgc atgggctgaa
     2641 aaactgacag gtattaatgc cgaaaccatt cgtgggctgg cgcggcagat ggcggcgaac
     2701 agaacgcaga ttattgctgg ctggtgcgtg cagcgtatgc agcacggtga acagtgggcg
     2761 tggatgattg tcgttctggc ggcgatgttg gggcaaattg gcctgccagg tggaggtttt
     2821 ggttttggct ggcactataa cggcgcaggc acgccggggc gtaaaggcgt tattctgagt
     2881 ggtttctccg gctctacgtc gattccgcct gttcacgaca acagcgacta caaaggctac
     2941 agcagcacca ttccgattgc ccgttttatc gatgcgatcc tcgaaccggg gaaagtgatc
     3001 aactggaacg gtaaatcggt aaaactaccg ccgctgaaaa tgtgtatttt tgccggaact
     3061 aacccgttcc atcgccatca gcagatcaac cgcattattg aaggctggcg caagctggaa
     3121 acggttatcg ccatagataa ccagtggacc tcaacctgcc gctttgccga tatcgtgctg
     3181 cctgcgacca cgcagtttga gcgtaacgat ctcgaccagt acggcaacca ctccaatcgt
     3241 ggcattatcg ccatgaaaca ggtcgtgccg ccgcagttcg aggcgcgcaa cgacttcgat
     3301 attttccgcg agctgtgccg tcgctttaat cgcgaagaag cctttaccga agggctggac
     3361 gaaatgggct ggctgaaacg catctggcag gaaggtgtac aacagggcaa aggacgcggc
     3421 gttcatctgc cagcgtttga tgacttctgg aataataaag agtacgtcga gtttgaccat
     3481 ccgcagatgt ttgttcgcca ccaggcattc cgcgaagatc cggatctcga accgctgggc
     3541 acgccgagtg gcctgattga gatctactcg aaaaccatcg ctgatatgaa ctacgacgat
     3601 tgtcaggggc atccgatgtg gtttgagaaa atcgaacgct cccacggtgg gcccggctcg
     3661 cagaagtatc cgttgcatct gcaatctgtg catccggatt tccgacttca ctcgcagtta
     3721 tgtgagtcgg aaacgctgcg tcagcaatat acggtagcgg gcaaagagcc agtgttcatt
     3781 aacccgcagg atgccagcgc gcgcggtatt cgtaacggtg atgtggtacg cgtgtttaac
     3841 gctcgcggtc aggtgttggc aggggcagtg gtttctgacc gctatgcacc cggcgtggcg
     3901 cgaattcatg aaggggcatg gtacgatcca gataaaggcg gcgagcctgg tgcgctgtgc
     3961 aaatacggta accccaacgt gttgaccatc gacatcggta cttcgcagtt ggcgcaggcg
     4021 accagtgcgc ataccacgct ggtggaaatt gagaagtaca acggaacagt ggagcaggtg
     4081 acggcgttta acggccctgt tgagatggtg gcgcagtgcg aatatgttcc cgcgtcgcag
     4141 gtgaaatcat gaccacgctg acagcacaac agattgcctg tgtttacgcc tggctggcgc
     4201 agttgttctc ccgtgaactg gacgatgaac aactgacgca aatcgccagt gcgcagatgg
     4261 ctgaatggtt ttcgttgctg aaaagcgaac cgccgctcac tgcggcggtg aatgagctgg
     4321 aaaaccgtat tgccactcag acagtacgtg acgatgctcg tctggaactg gccgctgact
     4381 tttgcggcct gtttttgatg accgacaaac aagctgcgct gccgtatgca tcggcctaca
     4441 aacaggacga gcaagagatt aaacgcttgt tagttgaggc agggatggaa accagcggca
     4501 atttcaacga accggcagac catctggcga tctatctcga gttgttgagt catctgcatt
     4561 tttcgctggg agaggggacc gttcctgcgc gaagaatcga caatctgcgg caaaaaacac
     4621 tgacggcgct gcgacaatgg ttaccagagt ttgctgcgcg ttgccatcag tacgacagtt
     4681 tcggttttta cgcggcacta agccagttat tgctggtgtt agtggaatgc gaccaccaaa
     4741 acagataacg tcgtttgttg gcctgataag acgcgtttag cgtcgcatca ggcattagtg
     4801 cgcagttgcc ggatgcggcg agaacgcctt atccggccta cgggaacagt gctttttgtg
     4861 ggcctgataa gatgcgttaa gcgtcgcatc aggcattatg gcgcagttgc cggatgcggc
     4921 gagaacgcct tatccggcct acgggaacag tgctttttgt gggcctgata agacgcgtta
     4981 agcgtcgcat caggcaacaa actcacgggt gagccacaaa ccgggaaagc cgctggcgca
     5041 gcaggcggtt ttcctgcttc aggtgcgcaa tatcatccat taacgtcagc gccaccgcga
     5101 tccccggcca gtccagagcc agttcatgac gcaggcgtac cgcgcgttgc accacaatgg
     5161 cggcatggtc gtcaaatacc caggttgtct cctggatctc ccgcggttca accaccccca
     5221 aaccgacaat ttcattcaac tcctcttcag agatgccggt atgcaggcaa aattcggtaa
     5281 tagtaaaagt caccgtaaca ttagccatta tgctttcccc caatctttac gtggatcaaa
     5341 agacgactgg gcgtctgcca gttgctgcca cagcgcggca gtgttttcat ccggtttcgg
     5401 cggcatcacg attttcagta ccgcatacag atcgccggtc tgttttttgc tcaccagacc
     5461 tttgccttta acgcgcaatc gttgcccggc ctggctgcca ggcgggatag tcagcaaaat
     5521 gctttctttc agtgttggaa cggtgacttt agtacccagc gccgcttccc acgggctaac
     5581 cggcaccact atttccagat cctggccgac aatatcaaac agcggatgtg gcgcaatgtg
     5641 aatcaccagc cacaggtcgc catttggacc gccgttttcg cccggcgtcc cctggccttt
     5701 caggcggatg cgttgaccat tgccgacgcc cgccgggatc ttcacattca gcgttttcgg
     5761 gatttcctgt tcgatcatgc caaaggcgtt ataaaccggc aggttatagc tgatggtacg
     5821 cttatgctca gtaagcgttt cttcgaggaa aaccgccact tcgatttcaa tatcgtggcc
     5881 gcgtgtggcg gggcgttgac ggctctggcg ggcatgctga ccgaaaattg acgagaagat
     5941 atcgtcaaaa tcttcggcgt taaaactctg accgtcgcca tgatggaact gacggttaaa
     6001 ttgcggatcg ttgcgatgtt gccacatctg atcatactca gcgcgacgtt gttcatcact
     6061 taacacttcc caggcttcgg cgacctcttt gaagcgggct tcggcatccg gttctttgct
     6121 gacatcagga tggtatttgc gggccagtcg acgataggcg gtcttgattg tcttgagatc
     6181 gtccgtcggt ttcacgccca tgatggcgta ataatccttt aattccatag cgttatctcg
     6241 cgtaaatcaa cacaaattga aggaacccct gtaaggtaac tcctataagt gtagggtaat
     6301 cctcaaaatt tcatatgcca acacagaata tgttattgaa atcatcgcgg aggggaggtc
     6361 gcctcaaggt gggaggaaca tattttattt acctccctcc tgaacttttg gctcatgctt
     6421 acaagttaaa caataaggaa aggaa
//
LOCUS       DAZGAP010000240         6411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGAP010000240 DAZGAP010000000
VERSION     DAZGAP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.241"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            109..324
                     /locus_tag="ACHLAM_004695"
     CDS             109..324
                     /locus_tag="ACHLAM_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7856108.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            498..755
                     /locus_tag="ACHLAM_004696"
     CDS             498..755
                     /locus_tag="ACHLAM_004696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856109.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            737..928
                     /locus_tag="ACHLAM_004697"
     CDS             737..928
                     /locus_tag="ACHLAM_004697"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7856110.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1070..2089
                     /locus_tag="ACHLAM_004698"
     CDS             1070..2089
                     /locus_tag="ACHLAM_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO7856111.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2810..3025
                     /locus_tag="ACHLAM_004699"
                     /pseudo
     CDS             2810..3025
                     /locus_tag="ACHLAM_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3200..5170
                     /locus_tag="ACHLAM_004700"
     CDS             3200..5170
                     /locus_tag="ACHLAM_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO7856112.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5189..5980
                     /locus_tag="ACHLAM_004701"
     CDS             5189..5980
                     /locus_tag="ACHLAM_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO7856113.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1940 a         1406 c         1523 g         1542 t
ORIGIN      
        1 agtaacagga acatcagtaa gcgatgagat ggtcttcccc ctccttcacg acaaccttta
       61 attacgtcat gatggataaa ctttaaccac aaaagtaaga gggataccat gtctcaacag
      121 aaaatcaccg gcatcgatct ggctaaaacg aacttttatc ttttcagtat caatgctcat
      181 ggcaaacccg caggaaggat caaactttct cgcaatcaac tgcttaactg gctggtgcag
      241 cagcctaaaa tgactgttgc gatggaggct cgcgggcatc ccatcactgg gccagggaaa
      301 tcaggaaact cgaccatgat gtaatatttc ttccggcgca acatgttaaa gcttatcagc
      361 gacgccagaa aaatgattat gacgacgctc aggcaatagc tgaggcctgc caacatggaa
      421 ctatacgccc cgtaccaata aaaactctgg agtaacagga cgtccagact tttttgaaaa
      481 tgcgccggtt ggtttcgatg gaaaggacac aactgataaa tcacgttcgt ggacttcttg
      541 ctgaatatgg cattgtgttc agtaaaggcg caacagaatt acgccaaaaa ttaccggcat
      601 tacttgagga tgcggaaaac gaactcactg ataccatgaa gacgttattg catcggcagt
      661 atatacggtt gattacacta gataatgaac tggagtggta tgattctgaa ctgaaaaata
      721 tgtccgccaa gatcctatgt gcaaacgctt gttagccatt cctagattca gccctctggt
      781 tagtcaggca ataaaaagct ggatggggga tggaaaacag tttagacgag gaagagatgc
      841 atctgcgact ctgggactgg ttccccgaca gttcagcaca ggtgggaagc aggttcttct
      901 gggtataaca aaatttggag aaatgtaatg aacacatccc accccattaa gtcaggtgct
      961 ctgtgatgga tgaagggaaa tactggaagg tgtggagcct cgcagatttc cggcatcata
     1021 gtgcccaata caggatgcat aaagctccct cagactggag actccacaaa tgaaatatac
     1081 accggttggc gttgatatcg caaaacatct gattcaggtt catttcatcg acgagcacac
     1141 aggtgaagtg attgataagc aactacgtcg tcaggatttt ctgatgttct tctgtaatcg
     1201 ggaaccctgc ctgattggta tggaggcctg tagaggctct cagcactggg ggcgtgaact
     1261 gacaaagctg gggcataaag tccggttgtt acaggcccac tttgttaagg cattcgtcat
     1321 gggaaataag aatgatgtga tggatgcccg ggctatctgg atggctgttc agcagccagg
     1381 taaggacatc gcggttaaaa cagaagaaca acagtcagtg ctggttttgc accgttcccg
     1441 cagacaattg gttaagttcc gcaccatgca gattaattct ctgcacggca cgctcctaga
     1501 atttggtaaa accattcaca aaggtcgggc agcgatggag cgagagctcc ctgaagcact
     1561 ggagcggatg aaagagagac tgccgccgta tctcattact gtgctggaaa accagtacaa
     1621 ccggctgaat gagctggact cgctgataga ggatattgaa aaaaagctta ccagcgtggc
     1681 gagacagaat gaaacctgta agcggttgct ggatattccc ggcgttgggc cacttattgc
     1741 aacggcagcg gtggccacca tgagggaagc atcagcgttt aaatcagggc gagagttcgc
     1801 ggcttatgtt ggtctggtgc ccaaacagac tggctcagga gggaaaatcc gcctgctgga
     1861 gataagcaaa cgtggtaata catatctgcg agccttgttt attcatagcg caagggcggc
     1921 agcattactg cccaaagaac ctggtccgtg gataaccgaa ctgaaaaaac gtcgtccaac
     1981 cagcgtggca atcgtcgcca tggcaaacaa actagcacga acagtatggg cgatagcagc
     2041 tcatggaagt aaatacgaca agaatcatgt cagtatcaga tcatattaat cgctgatacc
     2101 gataaatatt aacttttaat gaaaggatga atactgaaag gtaactctgg cggccatagt
     2161 gatgacaacg acaggtaaga ccgggactca ctaaacctga atattgtgtt gagctaaaag
     2221 ctcgctggag aaatgaggtg tgagtcagcg aattacatag gggctcgcag cttcatggct
     2281 gcaaaaaagc cggatataaa gctgcaacct acccgtcact gtcaaggcaa tggatgcctt
     2341 gcaacgggat gtgttcatat aatcaatatg tcagatctat gctgatacag ggtgccagag
     2401 cggtattgtt cagagcctca gagaaaactg attcacttcg tgtgtgggtt aattgtattc
     2461 gggaaaagcg tggcttcaac caggcagttg ttgctcttgc aaataaactc cttcggatcg
     2521 cctgggcaat tatcgcccgc aatgagatgt atttgcccgc agcaaaacaa aatcaataat
     2581 ctacgtaaaa tcagtaggag taaagcttac cctgaaattg caaagaatgc agaatgatga
     2641 aaaacaggtc agaccgacgt tacgaaaacc tgcaagatga gccaggccag taaggccgag
     2701 taagcgataa ggtcggagcg cgcgaatgtc tcatcaaggc cagaagcaat aacttcatga
     2761 acaaaccgga tatacgaaag taaacctgtc cttatcatag ttgctgtact cttgcaatac
     2821 gggggaagac catctcatcg cttacgaaca attcgtgaaa acgatgaaga aagactacat
     2881 cgcgtgcatg ccgaaatcta atgtaagaac ggcattgcat aatcttgcag tggagatcgg
     2941 gtattacaat gaaaaccatc cgcatagtgc gttaggttat ctcccaccaa gggaatatca
     3001 acgtcagcga ataacgtcac cttagatgca gtagttgtca ggaaatagtg actcaagggc
     3061 aggcttacca tatacactct tagtggttcg actgggtatt agaatagcta ttaacagatt
     3121 agcactgaac actgcgtaat agtggttgca caaacaaaat gttatgttat aacattgctg
     3181 tattgacata tattttcgaa tgtatatgaa cgtaatcaga actgtcattt gtacattaat
     3241 catacttccg gtggggttac aggcagcgac aagtcattct tctatggcta aagatacaat
     3301 caccgttgtc gcgacaggaa atcagaacac ggtatttgaa acgccgtcga tggtcagtgt
     3361 catcacgaat gacacgccat ggagtcagaa tgcggtcaca tcagccggta tgctgaaagg
     3421 tgttgcgggt ctcagccaga ctggtgcagg acggaccaat gggcagacct ttaatctacg
     3481 cggctatgac aaaagcgggg tacttgttct tgttgacggc gtccgtcaac tcagtgacat
     3541 ggcaaaaagc agtggcactt atctggatcc ggcactcatc aaacgcatcg aagttgtccg
     3601 cggaccaaac tccagtctgt acggcagtgg cgggctgggg ggggtggtgg acttcagaac
     3661 tgccgatgct gcagattttc ttccccccgg agagacaaat ggtgtaagtc tgtggggaaa
     3721 tatcgccagt ggtgaccaca gcacaggctc ggggctcacc tggttcggta aaaccggaaa
     3781 aacagatgcg cttctttctg tcattatgcg taaaagagga aatatctatc agagtgatgg
     3841 tgagcgtgca cctaacaagg aaaaacctgc agccctgttt gcgaaaggct ctgtcagtat
     3901 aacagacagt aacaaagcag gtgccagctt gcgtctctac cagaataaca ccactgaacc
     3961 gggcaatccc actctgacac atggtgacaa cggcctgcgt gacagaaaaa cagcacaaaa
     4021 tgacgtgcag ttctggtacc agtactcccc tgcggataac agcctcatca atgtaaagtc
     4081 aacgttatat ctcagtgata tcactatcaa gacaaacggt cacaacaaaa cagcagaatg
     4141 gcgaaacaac agaacctccg gtgttaatgt tgtcaacagg agccatactc tgatttttcc
     4201 gggagcccat cagttaagtt atggcgccga atattaccgt cagcagcaga agccggaagg
     4261 ctctgccaca ctatatccgg aaggaaacat tgactttaca tcgttgtatt tccaggatga
     4321 aatgacaatg aaaagcaacc cggttaacat tatcgtcggt tcccgttatg accggtacaa
     4381 gagcttcaat ccccgagccg gaaaactgaa agccgaacgc ctgtccccca gggcggcgat
     4441 ttcagtctca ccaacagact ggctgatgat gtacggctcc atatcctctg cattccgtgc
     4501 ccccacaatg gcagaaatgt acagggatga tgtacatttt taccgcaagg gtaaacccaa
     4561 ttactgggta cctaacctta atctgaaacc agaaaataac attacccgtg agattggcgc
     4621 aggtattcaa ctggacggcc tgcttacaga caatgaccgg ctgcagttga aaggcggata
     4681 tttcggaacg gatgccagaa actatattgc cacgcgcgtg gatatgaaac ggatgcgttc
     4741 ttattcttat aatgtatccc gggcccgtat ctggggatgg gatatgcagg gtaattacca
     4801 gtctgattat tttgactgga tgctttctta taaccgcacg gaaagtatgg atgccagtag
     4861 cagggaatgg ctgggctccg gcaatcctga cacactcatc agtgacatca gtatacctgt
     4921 tggtcatgga ggagtttctg ccggatggcg tgctgaactt tcggcagcag ccacgcatgt
     4981 gaaaaaaggc gatccccatc aggctggcta tgccgtacat tccttttcac tgtcttataa
     5041 gcctgtaagt gttaaaagct ttgaggcatc agtgactctg gataatgcct tcaacaagct
     5101 tgccatgaat ggcaaaggtg tgccgctttc aggcagaact gtcagtcttt atacccgtta
     5161 tcagtggtaa cctcgcagga aataaatcat gatgaaaaat acaggctata tcttagctct
     5221 ttgtctgaca gcatcggagc atgccctggc ccacgatgtc tggattaccg gtaaacaggc
     5281 agagaacaac gttaccgcag agattggtta tggtcataat ttcccctcaa agggcacaat
     5341 tcctgacaga aggaatttct ttgaaaatcc ccggctttat aacgggaaag agataataac
     5401 gctgaagcca acgtccacgg attatgtcta taaaactgag tctgcagcca acggtaatag
     5461 ctacgttctg tcaacgaaaa tgaaaccggg atactggtcg agaacctcgt caggatggaa
     5521 gcctgtcagt cgggagggca gaaatgatgt ggcatactgt gagtttgtga ctaaatatgc
     5581 aaaatctttt attcctggtg aacagcagat gccagcacaa ctctatcagt ctccaacagg
     5641 ggatgagctt gaaatcattc cattatccga tatcagtcgt ttcggtgaag atgtgaagct
     5701 gaaagttctg tataaaacat ccccgcttgc cggagctact ctggagcttg actctgtcag
     5761 ttatctgaaa tcatcccgtc atacccatgc agctgagcat aaacattcgg ctcataaagc
     5821 agaacttacc tttgtaagta atgaggacgg tatcatcact gtaccttccc ttcatgtcgg
     5881 gcaatggctg gctaaagtca aaaataagaa agtatttccg gacaagagtc tgtgtgatga
     5941 aactgtcgat gtggcaacct taagcttctc ccgaaattaa taatactatt cctgccctga
     6001 tttatgggca gggtattatc aatacgagaa tagcctaccg acatccgtgt aaaaaaccac
     6061 ataatgattt ttcatatcca tttccagata tacttatgta aagatcacct ccaaaaaagc
     6121 gtaatagtaa taaaaatccc tgaagcaccc tgttcaaacc agaaggaata cataacaaga
     6181 caataaaatg tgaaatccat cacagcaaag aactctttgt ttataatata atgaaaatta
     6241 gctaatattc tttaacaaga actgttggtc atataaaatt aaatattttt atgctaaatg
     6301 gttccatacg tgaagttata tcagaataag gagacatagc cagaaaactt tcttttcata
     6361 aactctttaa tattcaaaag aaaatattat acccagcatt atttatttga a
//
LOCUS       DAZGAP010000241         6351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGAP010000241 DAZGAP010000000
VERSION     DAZGAP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.242"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2764
                     /locus_tag="ACHLAM_004702"
     CDS             <1..2764
                     /locus_tag="ACHLAM_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7856114.1"
                     /translation="DWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVILAQK
                     ADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFNRLAA
                     ADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDGGADI
                     SGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESRTFSI
                     GQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGTSAVG
                     GASGSTTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSSALTV
                     SDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSGEYDF
                     MTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFHTLTA
                     GSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLVTAPA
                     GTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDAETPN
                     PVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVNSSAV
                     RDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNYTH
                     VQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDSGAYID
                     LIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELVYGAV
                     SGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLAN
                     GETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDNAVNA
                     SFRYSF"
     gene            complement(3332..6004)
                     /gene="rcsD"
                     /locus_tag="ACHLAM_004703"
     CDS             complement(3332..6004)
                     /gene="rcsD"
                     /locus_tag="ACHLAM_004703"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO7856115.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1542 a         1465 c         1763 g         1581 t
ORIGIN      
        1 tgactggatg gtgaacggac ttgcgggaga cgcacttcat aaaattggta agggaacact
       61 ggtggtggcc ggcagtggtg aaaatcctgg caccctgaat acgggggacg gaacggtcat
      121 tctggcacag aaggctgatg ctgcaggcag ggtccgggca ttcagtgaag tcagtattgt
      181 cagcggacga ccagttgtgg tgctgcagga cagtcatcag attgaagggg accgcattcg
      241 ctggggatac cggggaggta cgcttgatat caacggaaat gacatagcct tcaaccgcct
      301 ggctgcggct gatgaggggg cagtactcac aagccgggcg cgtcctgcca cagtccgtct
      361 ggatttcagc cagtcagggc agaaggccgt gatgtggcat ggtcatttta ccggtaacct
      421 cagcgtcctg aacaacacgt catctgcggt ggattttatc atggatggcg gagcggatat
      481 atccggcagt tttacacagc agggcggggg actgtatatc caggggcatc cggttgttca
      541 tgctgtcagc agtgaggcgg ttgcgactgc attgcgaaag cagggtgata attctgttct
      601 gacgcagccg gtatcatttg ctcagaagga ctgggagagc agaacgttca gcatcggtca
      661 actgaaactg aagggggcgg cattcagcct ttcccgcaat gcgacactga cgggagatat
      721 tgatgctgat aatgccacca tggtgctggg aagtgactcg ctatatctgg atatgaagga
      781 cggcacgggg agcagcagtg cgccggttaa ggggacgtct gccgtgggcg gtgcatccgg
      841 tagcactaca tttcgcggga atgtgaatat gcgtcattct gccctgacgg tccgcgacca
      901 ttttactggc agtataacgg catcggacag ccggatagct gtgagttcag agaatgtgcg
      961 tctggaaggg aattcccgcc tgacctcttc tgcacttact gtcagtgacg gcgggcgttt
     1021 acatgtgaag gggggactgg agacagatgg tggtgtgaca ctggacagag gaaccctttt
     1081 ggtggacggg ggaagtgtca ggaatgatgt atatgaacgt ctgctggcct ggtcagagga
     1141 gcgtggcggg ctgaacggaa gcggtgagta tgattttatg acgggggcag ctggtctcct
     1201 gcggagttat gtcagaggga gtgcaggtaa tgtgaatctg cagaatgcgg cctggatgat
     1261 gacgggaaac tcatccgtga agcatcttga gtcatcggga agtgctctgt atttcagcag
     1321 gccgggcggg gagttccata ccctgacggc aggcagtatg gatatcagcg acagtgttct
     1381 ggtgatgcgg acggacctta accactcaga ccagcttcgg gtgacagaat cgctgcgggg
     1441 taagaataac ctgttgttgg ttgattttac tgagcgttct gacgggcaga aggcgctgaa
     1501 tatccctctg gtgacggcgc cggccggtac cggtgcggat gtgttcagtg tgaaaacgcg
     1561 tgataccggc ttcagtcata tcacacctgt tgtcagggct gaacagggga cgggcggcac
     1621 ggcctggcag ctgaatgttg tgcagccgga aactgccgca gagcctgtgg cgacgcggca
     1681 ggatgcagag acgccgaatc ctgtggaagc tgtatcaccg ctcccttcac cggtttctgc
     1741 tccgtcatcg gctccggagg cgtcagtgac ggatgtactg accggagaga atgcggggga
     1801 atcaggtgag taccgtgatg agacccggtg gctgctgaca ggataccgga gtacggttaa
     1861 tagctccgcg gtcagggatg cggggatgct gatgtcaatg gggcaccgta acttcattaa
     1921 tgaggtcaac aacctgaaca aacgtatggg tgacctgcgg gacatcaacg gcgaggccgg
     1981 tgcatgggcc cgtatcatga gcggaaccgg ctctgccggc ggtggcttca gtgacaacta
     2041 cacacacgtt caggtggggg cggataaaaa acacgaactg gacggtctgg acctcttcac
     2101 cggggtgacc atgacatata ccgacagcaa cgccggcagc gacaccttca gtggtaaaac
     2161 gaagtcagtg ggtgcgggtc tgtatgcttc agccctgttt gattccgggg cctatatcga
     2221 cctgattggt aagtatgttc accatgataa tgagtatacg gcgtcctttg ccggactcgg
     2281 cacaaaagac tacagctctc attcctggta tgccggtgct gaagtgggtt accgctatca
     2341 tgtcacagag gatgcctgga ttgagccaca ggcggaactg gtctatggtg ctgtctccgg
     2401 gaaacagttc agctggaagg accagggaat gaacctcacc atgaaggata aggactttaa
     2461 tccgctgatt ggccgtaccg gtgtggatgt gggtaaatct ttctccggta aggactggaa
     2521 agtgacggca cgtgctggtc ttggctatca gtttgacctg ctggcgaacg gagaaacggt
     2581 actgcgtgat gcatccggag agaagcgtat taagggcgag aaagacggcc gtatgctgat
     2641 gtctgtgggt ctgaacgcag aggtccgtga caacatccgt tttggtctgg agtttgagaa
     2701 atcagccttt ggtaagtaca acgttgataa tgctgtcaac gccagcttcc gttattcatt
     2761 ctgacggttg ttactgaagg ggattatggc ctgacggctc cctgtaaata aagacaggca
     2821 cagaagattg tgagttactc agtgttccac caaaaagtaa ggtcgcaact ctgtgcctgc
     2881 ttcgtaagtg tgcagtgagg gccatatggt tataactgaa gcatccgacg acgtagtgtc
     2941 taccagagtg tcagatatgc aggaaaatat gagttccgac agaaatgcag taaaaaaagg
     3001 agtctcgcac tcccctccat aaaactccag ggctgtaagt tgtattaaaa agctgatttt
     3061 tctttagcga tacagacctc aataagtata acacccttca gttactgtgg ttatgcacag
     3121 gtgttatttt taacagtgtg gataaacagt gtcgattatt cagattggtt atggggcatg
     3181 tatgggacat ttttaatcgg cgaaatacgt cgaagttcgt cgatatggta aacgaatcat
     3241 ctatccaacc cttgaaaaac ggcgctcctg gacgatcttc gtcgattttt aaaatgcaac
     3301 aagtacgttg ttcatgtaat aggctacctt gctacagcaa gctcttgaca taactgtcaa
     3361 tgtcgctgat gtatttttct attcctggaa catccttctc acgaatcaga tgttccagcg
     3421 tttcacataa ctgcttgccg ggtaccagat ttagcatggc aaatacgcct ttaagacgat
     3481 gagccgtttg ggctaacgca gcaaagtcac tggtcgctgc ttcagtatac agcctcttaa
     3541 catcatccgg tactgtgtct acaaagagcg cataatagcc gctggcatgg agttgcgcat
     3601 tttcatctcc gcccagaggc gattctgtca cctcttcctg cgccagttgc acttcaatta
     3661 attgtaagac cgcttcctgc atagcgttgc tcatattgaa gttgacgcac aattgaccag
     3721 gcccaatttc ccgtacgcca gactcatcat cgcttaaaag caagccagag gcagtaagat
     3781 tagacggatt atccgttaaa aagatatcat aatcttgact aattaatctt tcatcgggtg
     3841 tgatacaggt tgcaccccaa ttttctaact ggcgagtgac aatattccga atttctgccg
     3901 aagtaacatc caccattacg cagacatcat ccagtaaacg ctcttcttcc tcttcaactt
     3961 ccgggtcagc tgcgagcatt ttgatatgca cagagtagcg agtaccaagc ccatcccgcg
     4021 ttttgatgtt taaatgaccg cccagtttac gcgccagttg atcgctcagc cagaatgcca
     4081 gcgggtccgc cttgccatag cgatcgtttt gggtctggtt gataaacggg aagtgcaaat
     4141 tatccatttc atgaatgctt acgccttctc cagtgtccag aatacggaac gtcaggcggt
     4201 cttcggagga ctcatcctga tcaacctcaa gggtgatttt tcccaattgc gttgaggtca
     4261 cggcatattg catcagtaac agcaaaatac gtcgtaaggc atcgcgatcg ccgcggcgca
     4321 tgtcgtgtgc tttcagatga ttgttaatca gcagttgcag acctttacgc ttgatggcag
     4381 gcaacactga aggcacaact tcatcaatta aatcctgcac ggagaacagc accgtctcac
     4441 ttttccagct atcgtccgca agcatgttcg ctaactgtat ttcatcgacc agccgcacca
     4501 gcacatccgc ctgattcgtc agttgtttgc cttccggggc gttgagttta gccgcgctct
     4561 ccgccaggga ctgtgcgggt tctttcagcg catcgccaat gtttttcata aaggtcatcc
     4621 gcccctgctg gtttttctca tacagacgct gcgcctgctt gagtttcttg tttaccagca
     4681 cttcgcgatc ctgatcgcga ataatgaaaa tttgtgtgcg cggcgcaacc tggctgcgga
     4741 acatgcggat ctcatacagc tcgttattga tcgtcgcctg aataatcccc tgatgctgtt
     4801 ccgccatggt ggtgatgttt tgcagattca aatgcggcag caaatgatcg gcaattttgt
     4861 tacttatgac agtgcggttc gattcctgat cgtgaaccag caggccgagc ggcagcagtg
     4921 agactatctc ttcattgatt gcccgtaaaa tgcgcaattc gttattgacc gccgtgttgg
     4981 gtagactttc tgtactgcga ctggagaaat ggcgaaatgt ggtatagcca aataacgcca
     5041 gcgccagcaa accgatgttc agcagcagtg gcagcagaat gttttgcaac gtatccagca
     5101 ataaggtgcc ataaggaacc tgccagacca ggcgcatatc ggtagagttg agtgccgagg
     5161 agatttcaat cttcgtactg ttaaagtgga tactgacgct atccgtccct tctttctcat
     5221 tatcattgtc tcccgttgcc gtcgcgtctg gctcaaggcg gaaactgtcc agcggcatac
     5281 ccggtgggat caaatcatta atcggcagat caaaagccac aaccgttgcc agatgtcccg
     5341 gctggttgaa cgtggtacgc aaggtaaagt aatgaccgtt ctgccaggcc aggcggcgca
     5401 tgttagaaaa gctttcgcgt tcatcgaggg cgttggcctg ttgcaacatc tctgcacgac
     5461 gtgaatcaac aatgtcgctg acggtcgatt ctttaaatcc ggaggtgaga tctttgaggg
     5521 gcagggttga gatcagcacc agactgttat cctgaccatt caggtaatac atcgaccacg
     5581 gtacattttc tgcgccccac aatgtatcca gataagtgga catccgttga gtcatctcaa
     5641 gcgttgagtt gtcgtgagag ccaaagatta acgcttccgt tttgcggcgt ggtttctcca
     5701 gatagtagac atcctgtttc aggcgcgtct cttgtaaacc ttcgccggag gagggggtgg
     5761 tcgtcgcggc aatgttgtcg tagatctgcc aggtcacgta acgccaggta tcgacgcgct
     5821 tttggatagc gtgggtaatg tcgacaatct ggtaactttt atctttcagc caggcgttaa
     5881 cggcgctttg taccattaca cccatcgtca ccagtaacac aatgatcaac agtaaaaaga
     5941 agcgggtaat gctccccggt aggagtgaaa agcgggtcgt ggccgttgtc tctttctgac
     6001 gcattcgtgt ttatgacctg ttaaaacttc gcgaagtgat agggcagtat aaagggtaca
     6061 gtgtgaaatt ccagactctt accggattga tgattctcta ttctcccgtg atatttatca
     6121 tcctgcattg ggggaaatag atgtacaagc gccattttgc tgtacaaata acctacaaaa
     6181 agcctaacga aattaattgc aaataaacga agattgctgg aaattatgct gatgttaatt
     6241 atttgtgaaa tagttaacaa gcgttatagt ttttctgtgg tagcacagaa taatgaaaag
     6301 tgtgtaaaga agggtaaaaa aaaccggatg cgaggcatcc ggttgaaata g
//
LOCUS       DAZGAP010000242         6344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGAP010000242 DAZGAP010000000
VERSION     DAZGAP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.243"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..401
                     /locus_tag="ACHLAM_004704"
     CDS             93..401
                     /locus_tag="ACHLAM_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856116.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFILILTVMNNLT
                     INYGTETSPELSGISYEDANALITELILFIEDVDAENHEAYQGHLNEITRILNFAIAA
                     "
     gene            complement(453..1043)
                     /gene="cobO"
                     /locus_tag="ACHLAM_004705"
     CDS             complement(453..1043)
                     /gene="cobO"
                     /locus_tag="ACHLAM_004705"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO7856117.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1040..1798)
                     /locus_tag="ACHLAM_004706"
     CDS             complement(1040..1798)
                     /locus_tag="ACHLAM_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO7856118.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2018..3067
                     /gene="sohB"
                     /locus_tag="ACHLAM_004707"
     CDS             2018..3067
                     /gene="sohB"
                     /locus_tag="ACHLAM_004707"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO7856119.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3103..3354)
                     /locus_tag="ACHLAM_004708"
     CDS             complement(3103..3354)
                     /locus_tag="ACHLAM_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO7856120.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3734..6331
                     /gene="topA"
                     /locus_tag="ACHLAM_004709"
     CDS             3734..6331
                     /gene="topA"
                     /locus_tag="ACHLAM_004709"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO7856121.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1645 a         1495 c         1678 g         1526 t
ORIGIN      
        1 attacgcgca caggtcatta gtgggttaaa ggcgtctgag gggctggcaa aattacttat
       61 tgccagcgga ataatgttat aggaggcttt ttatgaacga tgtgcaaaac cgtagaatac
      121 tggcattact tgatatgctg tataatactt tgggtaataa aaccacatgt aatgtttgtc
      181 ataaatattt tatccttatc ctcacggtga tgaacaatct gacgattaac tacggtactg
      241 aaacttcgcc agagttaagc ggaatcagtt atgaagatgc caatgcgtta attactgaac
      301 tgatattatt tattgaagat gttgacgctg agaatcatga agcatatcag ggacatttga
      361 atgagattac acgtattctg aatttcgcta ttgccgcttg aatataatca tgattaaagc
      421 acctcaagag gcttcttttg aggtgctttc acttagtagt caatccctat ctgcgctttt
      481 acaccggcat caaacgcatg tttcacgggg cgtaattcac tcaccgtatc cgccagttca
      541 agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg tggacgttca
      601 tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat atacgtcagt
      661 tcatcgagca aaaccatatc cagtgaagga tcggccagca ttcgtttggc atgttgccag
      721 acttcacggc aggccgcggt atcagattcg cgattttgtg tatcccaggt aaagcccgtt
      781 gccatcacct gaaactcaac gccatgtggc tccagtagat tgcgctcgcc attaggccag
      841 gtgcctttaa taaactgcac gatacccact tttttgccat ggcctactgc acgagttgcc
      901 gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat gataataccg
      961 cgctcatcct gggcctgggc cacacgagca tcgacttttt ctttcactcg ctgctgacgc
     1021 tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt tgggcgtcaa
     1081 aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag agcggcatga
     1141 tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctg gcacgcattg
     1201 cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga tattcatcgg
     1261 ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgcg ccccagttgg
     1321 ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg tccgatttga
     1381 gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg acctgcatga
     1441 cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg agcaatccgg
     1501 cattatgcaa aacaccatcc agacgcggat aattaacggc aatgcgctgt gccagttgtt
     1561 ggcaatcttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc tgacgtccag
     1621 tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg cccaacagaa
     1681 tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acggccaata ccatcgctgg
     1741 ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg taatgcatgg
     1801 ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt tggggcttta
     1861 tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa aatggatact
     1921 ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc tgttgtaaac
     1981 tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg
     2041 tttgtttttg gcgaaaatcg ttaccgttgt gctagcgatt gcggcgattg ccgccattat
     2101 tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca acaatctcag
     2161 cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact cacatcagca
     2221 aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag cagcaaaagc
     2281 gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg tgctggattt
     2341 taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa cggcggtact
     2401 cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg gtggcatggt
     2461 gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa acattccttt
     2521 aactgtaacg gtagacaaag tcgctgccag cggcggttac atgatggcct gtgtggcgga
     2581 caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggcgtggtgg cgcaaatgcc
     2641 caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca ccgccgggca
     2701 gtataagcgc acgctgacct tgctgggtga aaacaccgaa gaagggcggg agaaattccg
     2761 cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta tgcgtccgtc
     2821 tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg cggtagagaa
     2881 aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga tggaaggccg
     2941 tgaagtggta aatgtacgct atatgcagcg taaacgactc attgaccgat tcaccggcag
     3001 cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc aaaagccatt
     3061 gatgtaaaag acaaacgcga gggtaagacc tcgcgttttg ctttaatcaa ccaggtgata
     3121 tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg tgcttttggc
     3181 tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca cgccattacg
     3241 ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac gaatgatttt
     3301 gttggcctct ttcaacagcg tctcgcggtc gatgggttgt gtctctttgt tcattattta
     3361 ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat ataccacgct
     3421 ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag ttgcgtatcg
     3481 gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg cttgacattc
     3541 gaccaaaatt ccgtcgtgct atagcgcctg taggccaaga cctgttaact cagtcacctg
     3601 aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga gtccatatcg
     3661 gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa gaatcaaatt
     3721 aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa aagccaaaac
     3781 gatcaacaag tatctgggta gtgactacgt ggtgaaatcc agcgtcggtc acatccgcga
     3841 tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca ccaagacggc
     3901 taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg ttgacccgtg
     3961 gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg tctctgaact
     4021 gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg accgcgaagg
     4081 ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc gctatagccg
     4141 agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca aaccgggtga
     4201 gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc gcgtggtggg
     4261 gtatatggtt tcgccgcttc tatggaaaaa gatcgctcgt ggtctgtctg ccggtcgtgt
     4321 gcagtcggtg gcggttcgcc tggtggtcga gcgtgagcgt gaaattaaag cgttcgtacc
     4381 ggaagagttc tgggaagtcg atgccagcac gaccacgcca tctggtgaag cgttggcgtt
     4441 gcaggtgact catcagaacg acaaaccgtt ccgtccggtc aacaaagaac aaactcaggc
     4501 tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag acaaaccgac
     4561 aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg ccagcacccg
     4621 tcttggattt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg aagcaggcta
     4681 tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa atatggttcg
     4741 cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga atcagtacgc
     4801 cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg tcaatgtgat
     4861 ggcggaatcg ttgaaggata tggaagcaga tgcgcaaaaa ttgtaccagt taatctggcg
     4921 tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc tgaccgttgg
     4981 tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg gctggacaaa
     5041 agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag tcgataaagg
     5101 cgatgctctg acgctcgttg aactgacacc agcccagcac tttaccaaac cgccagcccg
     5161 tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgc ggtatcggtc gtccgtctac
     5221 ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgtgtag aaaaccgtcg
     5281 tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa atttccgcga
     5341 gttaatgaac tacgacttta ccgcgcagat ggaaaacagc cttgaccagg tggcaaatca
     5401 cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc agcagttaga
     5461 taaagctgaa aaagatccgg aagagggggg gatgcgcccg aaccagatgg ttctgaccag
     5521 catcgactgc ccgacctgtg gtcgcaaaat ggggattcgc acagcgagca ccggggtatt
     5581 ccttggctgt tccggctacg ctctgccacc gaaagagcgt tgcaaaacca ccatcaacct
     5641 ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa ccaacgcgct
     5701 gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc tcatcgatcc
     5761 gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg agatcgaaga
     5821 gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa aatgtggctc
     5881 tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacta acgaagagtg
     5941 taaaaacacg cgtaagattt tacgtaacgg cgaagtggca ccaccgaaag aagatccggt
     6001 gccattaccg gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc gtgacggtgc
     6061 tgccggtgtg ttcctggcgg ccaatacctt cccgaaatcg cgtgaaacac gtgcgccact
     6121 ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt atctggccga
     6181 tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc gtaaaaccaa
     6241 acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag cattttatgt
     6301 tgatggcaaa tgggttgaag ggaaaaaata acctttaatt ctgt
//
LOCUS       DAZGAP010000243         6304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGAP010000243 DAZGAP010000000
VERSION     DAZGAP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.244"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..475
                     /locus_tag="ACHLAM_004710"
     CDS             <1..475
                     /locus_tag="ACHLAM_004710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7856122.1"
                     /translation="ELTVQSASAKKKPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVV
                     NIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGDNPEYAIPQ
                     KVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILINRKDDE"
     gene            complement(578..665)
                     /locus_tag="ACHLAM_004711"
     tRNA            complement(578..665)
                     /locus_tag="ACHLAM_004711"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(629..631),aa:Ser,seq:gga)
     gene            898..1836
                     /gene="ghrA"
                     /locus_tag="ACHLAM_004712"
     CDS             898..1836
                     /gene="ghrA"
                     /locus_tag="ACHLAM_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO7856123.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            1891..2628
                     /gene="ycdX"
                     /locus_tag="ACHLAM_004713"
     CDS             1891..2628
                     /gene="ycdX"
                     /locus_tag="ACHLAM_004713"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO7856124.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            2652..3206
                     /gene="ycdY"
                     /locus_tag="ACHLAM_004714"
     CDS             2652..3206
                     /gene="ycdY"
                     /locus_tag="ACHLAM_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO7856125.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3308..3799
                     /gene="ycdZ"
                     /locus_tag="ACHLAM_004715"
     CDS             3308..3799
                     /gene="ycdZ"
                     /locus_tag="ACHLAM_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7856126.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(3863..4696)
                     /gene="csgG"
                     /locus_tag="ACHLAM_004716"
     CDS             complement(3863..4696)
                     /gene="csgG"
                     /locus_tag="ACHLAM_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO7856127.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(4723..5139)
                     /gene="csgF"
                     /locus_tag="ACHLAM_004717"
     CDS             complement(4723..5139)
                     /gene="csgF"
                     /locus_tag="ACHLAM_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO7856128.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5164..5553)
                     /gene="csgE"
                     /locus_tag="ACHLAM_004718"
     CDS             complement(5164..5553)
                     /gene="csgE"
                     /locus_tag="ACHLAM_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO7856129.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(5558..6208)
                     /gene="csgD"
                     /locus_tag="ACHLAM_004719"
     CDS             complement(5558..6208)
                     /gene="csgD"
                     /locus_tag="ACHLAM_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO7856130.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1612 a         1443 c         1564 g         1685 t
ORIGIN      
        1 tgaactgacg gttcaatcag catcggccaa aaaaaaacct tattgcgtca tgattatgga
       61 tattgaccac ttcaaaaaag tcaacgacac ctggggacat ccggttggcg atcaagtgat
      121 aaagacagtg gtgaatatca ttgggaaaag catacgacca gatgaccttt tagcgcgcgt
      181 tggcggcgaa gagtttggcg tcttgctgac ggagatcgat actgaatgtg cgaaagcttt
      241 agcggaaagg attcgggaaa atgttgaacg tttaactggc gataatcctg aatacgccat
      301 accacaaaaa gtgacaatta gtattggcgc tgttgtaacg cagaaaaatg agttaaaccc
      361 taaggagatc tatcaactgg ctgataacgc actttacgag gcgaaagaga ccgggcgtaa
      421 taaggtggta gtaaaagagg cagaaattct tatcaaccgc aaggatgatg aatgagtctg
      481 tcgctagttg tcgacctccg cctggggctt ctcatccccc agtgtgtgaa atatacgaaa
      541 aaaagctcgc actttcgtac gagctcttct ttaaatatgg cggtgagggg gggattcgaa
      601 cccccgatac gttgccgtat acacactttc caggcgtgct ccttcagcca ctcggacacc
      661 tcaccaaatt gtcgttcctg tcttgctgga acgggcgcta atttagggaa atcatgacct
      721 gaggtcaaca aactttttta aaaaatcgct cgattattca aacttcaatc aatatgcagt
      781 tttaataagc gaaatttgct ttttttgcca ccgaccacgg atttgttatg ctgctggtct
      841 ttgtagatca taacgataag tgcgaataaa tttcgccaac actcttcggg agtcagtatg
      901 gatatcatct tttatcaccc aacgttcgat acccaatggt ggattgaggc actgcacaaa
      961 gctattcctc aggcaagagt cagagcatgg aaaagcggag acaatgaatc agccgattat
     1021 gctttagtct ggcatcctcc tgttgaaatg ctggctgggc gcgatcttaa agcggtgttt
     1081 gcacttgggg caggtgttga ttctattttg agcaagctac aggctcaccc tgaaatgctg
     1141 aagccttctg ttccactttt tcgcctggaa gataccggta tgggcgagca aatgcaggaa
     1201 tatgctgtca gtcaggtgct gcattggttt cgacgttttg acgattaccg catccagcaa
     1261 aatagttcgc attggcaacc gctgcctgaa tatcatcggg aagattttac catcggcatt
     1321 ttgggtgcag gtgtactggg cagtaaagtt gcacagagtc tgcaaacctg gcgcttcccg
     1381 ctgcgttgct ggagtcgaac ccgtaaatcg tggcctggcg tgcaaagctt tgccggacgg
     1441 gaagaactct ctgcatttct gagccaatgt cgggtattga ttaatttgtt accgaataca
     1501 cctgaaaccg tcggcattat taatcaacaa ttactcgaaa aattaccgga tggcgcgtat
     1561 ctcctcaacc tggcgcgtgg tgttcatgtc gtggaagatg acctgctcgc ggcgctggat
     1621 agcggcaaag ttaaaggcgc aatgctggat gtttttaatc gtgaaccctt accgccggaa
     1681 agtccgctct ggcaacatcc acgcgtgacg ataacaccac atgtcgccgc gattacccgt
     1741 cccgctgaag ctgtggatta catttcgcgc accattgccc agctcgaaaa aggggagagg
     1801 gtctgcgggc aagtcgaccg cgcacgcggc tactaataaa gcatcaggtt tcctgctatc
     1861 cttggcggga attgaataca ggagagagtt atgtatcccg tcgaccttca tatgcatacc
     1921 gttgccagca cgcatgcata tagcacattg agtgattaca ttgcccaggc caaacaaaag
     1981 ggcattaaac tttttgcgat caccgatcat ggcccggata tggaagatgc gccgcatcac
     2041 tggcacttca ttaacatgcg tatctggccg cgagtggttg atggggttgg gatcctgcgc
     2101 ggcatcgaag ctaacattaa aaatgttgac ggtgaaattg actgcagtgg caaaatgttt
     2161 gattcgctgg atctgattat tgccggtttt catgagccgg tgtttgcgcc gcatgacaaa
     2221 gcgaccaata cgcaagcgat gatcgccact atcgccagcg gcaatgtgca tataataagc
     2281 catcccggaa atccgaaata tgaaatagat gttaaagccg ttgctgaagc agccgcgaaa
     2341 catcaggtgg cgctggaaat caataattcc tcatttttac actcacgtaa gggcagtgaa
     2401 gacaactgtc gggcggtagc cgcagcggta cgtgacgctg gaggttgggt ggcattaggc
     2461 tcggattctc acactgcgtt taccatgggg gaatttgaag agtgtcttaa aatcctcgac
     2521 gcggtagatt ttccgccaga gcgcattttg aatgtttctc cgcgccgctt acttaacttc
     2581 cttgaatctc gcggtatggc accgattgcg gaatttgcag acctttaatt actagtaatg
     2641 gaaatatatt aatgaatgag ttttctatcc tctgtcgtgt gctgggttcg ctctattacc
     2701 gccaaccgca agatccttta ctggtgccgt tgtttaccct gattcgtgag gggaaactgg
     2761 ctgcgaactg gccactggag caggatgagt tactgacgcg tttacagaaa agctgtgata
     2821 tgacgcaagt ctctgccgat tacaatgcgt tgtttatcgg cgatgaatgt gctgtgccgc
     2881 catatcgtag cgcatgggtt gagggcgcaa cggaagcgga agtgcgcgct tttctttccg
     2941 aacgagggat gccactagcg gatacgccag ccgatcacat cggcacattg ctgcttgcgg
     3001 cttcctggct ggaagatcag tcaacggaag atgagagcga agcactggaa acactgttca
     3061 gtgagtatct gttaccctgg tgtggtgcgt tccttggcaa agtggaggcc catgcaacta
     3121 cgcctttctg gcgcaccatg gcaccgctaa cccgcgatgc tattagtgca atgtgggacg
     3181 agctggaaga agattctgaa gagtaaatgt gatctgcatc actcataact gcaacgggta
     3241 atttgttgtt gcataaaatg tgtgctcgat ctcattcatg gccgcgtttt ctgctatcat
     3301 gcgcggcatg aacatacttc tctctattgc aatcacaaca ggcattctct ccggtatctg
     3361 gggatgggtg gctgtttccc tgggcttact aagctgggcg ggcttcctgg gctgtacggc
     3421 ctactttgcc tgcccacaag gtgggctgaa agggctggcg atctccgctg caaccctgct
     3481 tagtggcgtg gtgtgggcga tggtcattat ttacggtagt gcactggcac cacatctgga
     3541 aatactcggt tatgccataa ccggtattgt cgcgtttctg atgtgtatcc aggccaaaca
     3601 gctgctgctt tcatttgttc cggggacgtt tataggcgca tgcgcgacgt ttgccgggca
     3661 gggtgactgg aaactggtgt taccttcgct ggcgcttgga ctggtatttg gttacgcaat
     3721 gaaaaacagc ggcctgtggt tggcggcgcg tagtgcaaag acggctcacc gtgagcagca
     3781 aatcaaaaat aaagcgtgag gggcactcac gctttcgctt aaaccgtaaa atgtcggatg
     3841 ataattccgg catttttatc tatcaggatt ccggtggaac cgacatatgg cggtatttca
     3901 ctagaatgtc attctgccgt tctgctttat tttgcaaatc ccaaagaccg cggtcgatac
     3961 catcattaat caggaaaatg acgcctgttt cgatagccga catcaggcac agcataacgg
     4021 gttcgttcga ggtgtaacct acttcccctt ccagcaagcg ctggtagtca ataaagcgga
     4081 agacccccgc ctgaacttca taggaaagta tcgtcttact ggtgttcacc gaggagagga
     4141 tctcgccggt actcacattg acgacgcgca ggttcacggc aatctgatca agctggtatt
     4201 gcgtgtctgc accgatccca aaatatcttg ccccaacccc gcccgattta acgttgcttt
     4261 cataaccgat aattgaacct tcaaccatga tatttgccgc cgttaaagat tgcagcggga
     4321 ttcggttatt aatggcaacc gtgccgtttt cttgtgccgc acgaataatc ttgcgctcgt
     4381 taagcaggtt ttgtaagcct tggcgctcca gcggtataaa ccagcgagaa tctttcaatg
     4441 ccgtgaccag cattgccgtg gcgctttgcg gaacagcagt ggagaagtta cttgccgggt
     4501 agggtttaaa ttgcccggtt tcgtcctgaa tgttgtatac cgaaacaaag atcttacccg
     4561 tcggcgctgg cagatgggtc aaatctttgt agctctgagc acgaggcatt aatgtcggtc
     4621 tggcggcttc tttaggcggg gcggttaagc atccgctcag taacatgacg gcaaccaaaa
     4681 gaaataagcg ctgcatgatt attttcctta tgaagctggg gcttaaaaat cggttgagtt
     4741 attttgtaaa cccgaaacct ggatggtcga ggtttgtccg gttttacgat ctgtcacgtt
     4801 caactgcaat tgaccatcgc ggttagcaat atcaacaata taatcgttgg tcaccatgcg
     4861 gcccggttta ccggtattaa tattcgacag tagcccaccc aaaatttgtg actggatggc
     4921 ctgagtaaag ttatctaacg ccgagggcgt ttcaatacca aagtcatcgt tatagctcgg
     4981 atctttataa gagttttggg cctgagcgct atttaacaaa aaagcgccat tatttgggtt
     5041 accaccaaag tttggattac ggaactggaa agtcatggtt ccagcccaac ttaatggcga
     5101 aataagcatg agtagaacta ctgcatgttt gacacgcatt gcagcctccg aacaatttat
     5161 tatttaaaat tcatcatgcg ccaaatcgcc cgtacttaat aacgcttggt ttatctggcg
     5221 acgatttagt gcttcttcag tttgaatcag tgcaaagacg acagttttct cgaagtctct
     5281 tttcaacgga aataaaaaag tctggaaaat aacgtcctga ttcaccgtta ttgtgatcca
     5341 gcttccccat cgtgcactgg gcctttcatt aatcgttaag ttacccgtat agtcactttc
     5401 ccatttatca ctaaaggctc ggtaaaaatc atggccaata gatgaaacag tatggtcagt
     5461 taacaatccc gggacttcta cctcaacggc gtgaagattc cctgcggcga acagaaattc
     5521 tgccgccaca atccagcgta aataacgttt catggcttta tcgcctgagg ttatcgtttg
     5581 cccaggaaac cgcttgtgtc cggtttttta cggctatctt cttgaaaaga ttataaagat
     5641 gcgttttaac cgtattttcg ctgatgaaca acgaacgagc gatctcgtta ttagacgcgc
     5701 cgatacgcag cttattcagg atctcttttt cccgatgagt aaggagggct gattccgtgc
     5761 tgttataacg atagttacct gaatgcgtaa tcaggtaact ggcaagcttt tgcgtaaagt
     5821 agcattcgcc gcgcaggacg ccttgcaacc cattgacaac acgttcttga tcctccatgg
     5881 cataaaaaac gccgttgata tgaggccagt tttcaatgtc gcggtacggg taatcttcag
     5941 gcgtatttag caacaatatt ttgatattgt tgtttttcct gctcaaagta tcctgccaat
     6001 aatggataag ctttttatcc gcttccatca tatccagaag aataatagag cctgaagaga
     6061 tatcgtccag agaacgttga atattatgta attttcctgt aattgccagc gattgcttaa
     6121 ggtgctgcaa gagagctgtc gcctgcaaag aaggtttagt gatcaacaat aatgtatgac
     6181 catgaatact atggacttca ttaaacatga tgaaaccccg ctttttttat tgatcgcaca
     6241 cctgacagct gcctctaaaa tagaagcacc agaagtactg acagatgttg cactgctgtg
     6301 tgta
//
LOCUS       DAZGAP010000244         6300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGAP010000244 DAZGAP010000000
VERSION     DAZGAP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.245"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3649)
                     /locus_tag="ACHLAM_004720"
     CDS             complement(<1..3649)
                     /locus_tag="ACHLAM_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7856131.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PLTEAIPSPPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGIMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLYFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTNDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGADGYYNATLDYSDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAVHGQLSRYTAADGAVWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAWTDGNGVDWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYLLDNCGRVTEEHDWGGVTWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQ
                     SAGRHRISEETE"
     gene            complement(3669..4130)
                     /locus_tag="ACHLAM_004721"
     CDS             complement(3669..4130)
                     /locus_tag="ACHLAM_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO7856132.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRIDQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4158..6059)
                     /locus_tag="ACHLAM_004722"
     CDS             complement(4158..6059)
                     /locus_tag="ACHLAM_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7856133.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVRPPLWRA
                     GLRQNFRIFQNEDIRSILGTILQENDVTEWSPLFSEPHPSREFCVQYGETDYDFLCRI
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDWPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTEHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPKPKPSVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPANQDSSCWIRVAQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HEDNRSPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVMIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKQIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1155 a         1906 c         1743 g         1496 t
ORIGIN      
        1 gctcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccgcagc
       61 tgtcatagcc ataagtctca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctggccgttc cggtccagcc ggtactcccg gcgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacacct gacacgttac ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgacggaac cagcccgccc catgtggctt cttccggaaa aaatccggcg cgctggctga
      301 caggctggcc ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcatcgg
      481 ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtaatggta ctcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgat ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca ggccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggtcacgc cgccccagtc atgctcttcc gtcacccgcc
      841 cacagttgtc gagaagataa cggtatgact caccctgcgg gttgatgact tctgtcagtt
      901 gcagcgtgtc tttatcatag cggtactgcc agcggtggcc ttcaccgtcc gttcgcgcca
      961 ccggcaaatc aaacgggccg tattccattg tccagtccac accgttaccg tccgtccagg
     1021 ccagcaggtt gccgtggcgg tcatagcgga atccttctgc gccggttcct gcccgctcaa
     1081 ggccgctcag tttatggtgc tgctgccact gcaaccgcac tgactgaccg ccggggccgc
     1141 gcatcttcac cggcaggtcg tgaccactgt attcggtgtg gtgttcacgg cccatcacgt
     1201 cggtgagtgt ttccggccgg ttgtggtggt cgtactctat gcccagacgc cgttcatcct
     1261 ggcggaatat ccccgacagc agcccctgag cggtgtacgc cagccgggtc tcttcgccct
     1321 gtggcgatac cagactcacc ggcaggccgc gttcatcgca ctgctgtgtc cacgtctgcc
     1381 cggcagggtc ggtgatgttg ctgagcaggc cgcgctcatc atagcggtac aaccacaccg
     1441 cgccgtctgc cgccgtataa cggcttaact gaccgtgtac ggcgttgtat tcaaatttct
     1501 ccacccgtcc ggcaggggag tggcgggcca gcaggtgatg gaattcatcc cattcatacg
     1561 tggtggtgct gccgtccggc gcttcttccc gcagaatatt gccgtcagga tcatagtaat
     1621 aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc gtcactatag tccagcgtgg
     1681 cgttgtaata gccgtccgcc ccggtgacat tcacgcaccg gccctgttca tcgtaggtga
     1741 aacggctcca cgtctggtcg ttatcggacc agcggatgat ccggtccgca gcgtcgtact
     1801 cataaaacag gtggtagtcc agccgcgcat ccgcttcggt cagccgcccc cgcgcatcct
     1861 gttcgtaggt cgccagcgtc tgtatgccgt tatccgtgcg gtgaatggct ttcaggtagc
     1921 cgctggcgtg gtacgtcagt ttaagccgga tcccgtcggt gtgactgact cccgtgatgc
     1981 ggtactggct gtcgcgctca aacgtgatgc ggttgctggc ggtgtcgctc atcccgccca
     2041 gcatgtacat ccggtcaccg gggctgtgtg ccaggcggtc atcattggta tcgtcagtca
     2101 ccgggtggcg caggcgcatt cccggaaagg cgtcataaaa gaagcggctg ctcagggcat
     2161 cacggtcgaa cagctccagg taatcgtcgc ggcggcgcag gatatactgg gcgtgatacg
     2221 ggttaaccgc cgtatcactg tctccgtcga aggcaaagta cagcgtggct ccctggctga
     2281 tgatatccac ccggtttccc tgttcacgca cccaggcgta ttcgctccag gagtcgctcc
     2341 agcccacgcc cgtcaggcca tgcagtggca gtacagaact gtggaaacgg gtgaaggaga
     2401 gggggagtgt ctggcccagc gtaaagtcgg tacgttcgtc gcagtatgcg ccggttaccg
     2461 gatcgacagg atctgttacc catctgatga ctttattggc aatacttttt aacgacttac
     2521 ctgttttccc cagcaacgcg ccgagctttg cccccagcag cttcccgctt ttccccagca
     2581 ggtttcccag tttgccgagt ttcgccagat tccccagttt tcccagcagt cccgcgggcg
     2641 gcagaagata gctggctatc gttattcccc ccaggatcat tctctgccac ggcgggaact
     2701 ccggctggat atccagatag gtcacctgtt caccgccaat aaagacgtcc ggcgaacctt
     2761 ccacgattgc cgcgctgcac tccagtttgt cgcccttgcg cgcggcaggc tgattgttga
     2821 taaagacgtt gctgctgccc tgggctatcc tcggtggttt gttctccttg tcgcagagcg
     2881 ccgtgtcctc accggcttcc gggaaatcca cgtctttttt gatttcgcca cgcccgccca
     2941 ccatggtggc aaactccatt aataccggac cggcgggaaa cagaagcgat gcgccggcgc
     3001 tgacgcgatc cataataccg gcattgctgt ccgtcagggt gataacagcg ttcccccagg
     3061 ccttcgccag tggcactgct ttttccgctg ccgattcaag caggtcagtg gcaatatccc
     3121 cccatgaagg ttccccctgt ggtggagagg gtattgcttc agtcagggga ggagggggaa
     3181 gggtgacggc ggccctggct gctggcttac cgttaatttt tacgttttca gaaccggtat
     3241 tgatggcgcc gtccgtgtca caaaaactgt ttatccatga ctcaagctgg ttctgtttct
     3301 cctccacata atcactcgcc ttatagctgg catacatcag cgcaggcacc agaaccagcg
     3361 cataagggcc ggtggcgcag gcggccataa tcaggcaacc ttctgcgata gttactatcg
     3421 cccccagcac ggcccctgcc agtgccccca gaaaactttt atgctgaatt tcatcgccca
     3481 gcctggcggc aggtgggccg gacttctttg ctgccagcat ctgtgcaatg gctttgtcgt
     3541 tggctgcccg tttcatctgg ctggcgacca tcgcctgctg tgacagcatt cgcattgcca
     3601 gcgtaccgcc tgcggtcgct ccctgtgggc cgcctggtcc ttcactcatg tgttatccct
     3661 cgtttatgtc agcgcggctg gtccttttgg atgtcattct ccggtggcaa cggggtgaag
     3721 ctctttatca cctccagcag catcgtcttt tgactgttct tcagggataa ggaggtgctg
     3781 aaagtgaagg tcagcagcgt atctccacgc cgctggaata cggctatctg gtcaatccgt
     3841 ccctggttcc cctcatcttc cggaccgttc caggaataat ccagcagcca ggccggggcc
     3901 tctgccagag tgatttcccg gcaggcatgt tcggtgtacc catcaagttt gccctggtac
     3961 agcgccagcg tttcctggta cagctctggt gtcagggttc cctgtggaac ggcttcgcgg
     4021 gtaatgacca ggttgatgcc actgttatct gtggacacca ggacctgcat tgactgatcc
     4081 tgccattgcg atggcagctg aatatgacct tcattaaaag tgaactgcat aaaatactcc
     4141 ctgataatct gaatgaatca gttgatgaaa acagaaccgc ccacgatggt gatgccatcc
     4201 ttcgacagcc tgattaccgt cgattccccg acctgtagct gaatgccatc cggcgtcagc
     4261 gcaattttgc tttcctccac ggccagcata atttgcttaa ttgccgtata agcctgtata
     4321 ccgtcgcctt cgccaccatt gtttacagcg tcaacatgca ctgaaccggc aatcgtgcct
     4381 ctgtcctgaa aaacatcctc agataaagac ccttttagta cgcctgtgat tttgtggcct
     4441 ttttccaccg taagaaggcg gttaccttcc tggatcatga ccgtctggtc attttttacg
     4501 gtttcgcggt gatcgtgttt tacggtggtg gtacggtcgt tgagtacctc ggtatccatg
     4561 ttcttctggg cgtggatgta gacctgttct ttccctgtcg catcgtcaaa cttcagctcg
     4621 ttaaacccgc tgcccatata tgttttggag cggatggtca tctgcgtctt cgttcccggc
     4681 aggctgcccg gcgagcggtt atcttcgtgg taggtgcgcc ccataatgat cggctggtcc
     4741 ggatcgccgt tgaggaagtc cacaatcacc tcctgaccca cgcgcggtat cgccaggttg
     4801 ccaaatcccg gccccgccca cgcctgcgcc acccgtatcc agcacgaact gtcctgattg
     4861 gccgggttat agcggtccca gttgaatttc acccgcaccc ggccatgctc gtcgcagaag
     4921 atttcctctc ccgcaggccc cgtcacgacg gcgctctgcg gaccgtccac tgacggcttt
     4981 ggcttcggct gaggtcgcca cgtccggtct gccggtatta ccgcaaagtg gttattcagc
     5041 gtggtacctg accccctgcg tcccggcacc gcctgcggct gttcgccgtg cagttcgctc
     5101 gccaccacct gccattcccg gttcaggttc gcctgcggat gctccgtcag cacaattcgc
     5161 cgtccaggcc atatctccgg cgagcggctc attccccgcg cggtttcggc gttgtttcgc
     5221 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgcccgggc
     5281 cagtcataca cttcatactg tgtgcgctgg taatcctgat gctggccttc ctggtcaaaa
     5341 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacaga ggaaggacgg
     5401 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
     5461 gggttccagg ggatttcaaa ggattccggc agatagcgga ccgtgtcgca cagcaccaga
     5521 ctctggtcgg tacttttctg cgcgtgctcc tcatagaaga agatgccttc ctccgctgct
     5581 atccggcaga gaaagtcgta atcggtttca ccgtactgga cacaaaactc acgggaagga
     5641 tgcggctcac tgaacagcgg gctccactcc gtgacgtcat tttcctgcag tatggtgccg
     5701 aggatgcttc tgatgtcctc gttctggaat atacggaagt tctgacgcag tcctgcccgc
     5761 cacagcggcg ggcgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
     5821 tcaaaccagg tcaccacgcc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
     5881 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctgactgacc
     5941 agagaaaggt caagggaaaa aagtgaagag agtgactggg tgagatgaaa ggagaccacc
     6001 gcaaaagcgt ccggtggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
     6061 gcaacctccg tgaaaatgaa taataatccg catatattgc atgtacaaca tgtcgggtaa
     6121 tgagataagc gggccggctt caggcgttac cggggaatat cagaaaagtg tcatggttat
     6181 gtgatgattt tgtccgggat tttacttcag agaaacgggg ggctgttgac tcaacaaaat
     6241 aatcacaaac tcaactgacg ggttcacgat gttattaagg acaggctcgt atattacatc
//
LOCUS       DAZGAP010000245         6229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGAP010000245 DAZGAP010000000
VERSION     DAZGAP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.246"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            226..2073
                     /gene="ldtD"
                     /locus_tag="ACHLAM_004723"
     CDS             226..2073
                     /gene="ldtD"
                     /locus_tag="ACHLAM_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7856134.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2254..2802
                     /locus_tag="ACHLAM_004724"
     CDS             2254..2802
                     /locus_tag="ACHLAM_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO7856135.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2829..3476
                     /gene="gloC"
                     /locus_tag="ACHLAM_004725"
     CDS             2829..3476
                     /gene="gloC"
                     /locus_tag="ACHLAM_004725"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO7856136.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHLAM_004726"
     CDS             complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHLAM_004726"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO7856137.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHLAM_004727"
     CDS             complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHLAM_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO7856138.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1575 a         1525 c         1580 g         1549 t
ORIGIN      
        1 cgaaaactcc gtttctgcac taaaaaagtg gcacattacg gcgcgctttt ctttaaactt
       61 ctttacatta ggttatgtaa aaacgtatcg gcgtttatat actgaagata agcctgatga
      121 gtaacaggct tgctcgtcat actttcgtga gtattggcgt tgtacaggca agtcgtaaaa
      181 taacagcctg gctattcaga gtatgataaa aacagggggc aagggatgtt gcttaatatg
      241 atgtgtggtc gtcggctgtc ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg
      301 ttcaatgcgc aggccgatga gcctgaagta atccctggcg acagcccggt ggctgtcagt
      361 gaacagggcg aggcactgcc gcaggcgcaa gccacggcaa taatggcggg gatccagcca
      421 ttgcctgaag gtgcggctga aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt
      481 tataagccgg tttatcttaa ccagcttcaa ttgttgtatg ccgcacgcga tatgcaaccc
      541 atgtgggaaa accgtgatgc tgttaaagcc ttccagcaac agctggcaga ggtggcgatt
      601 gccggtttcc agccgcagtt taataaatgg gtagagttac tgaccgatcc tggtgttaac
      661 gggatggcac gcgacgtggt gctctctgat gcgatgatgg gctatctcca tttcattgca
      721 aatattccgg tcaaaggcac tcgctggcta tatagcagta aaccttatgc gctttcaacg
      781 ccgccgcttt cggtgattaa ccaatggcag ctggcgctgg ataaaggtca attgcctacg
      841 tttgttgtag gattggcacc gcagcatccg caatatgcgg cgatgcatga atcgttactg
      901 gccttactca gtgacaccaa accgtggccc caactgaccg gcaaagcaac gttgcgccca
      961 ggacagtgga gtaacgacgt accggcgttg cgcgaaatat tgcaacgtac aggcatgttg
     1021 gacgggggac cgaaaattac gctacctggc gatgacacgc caactgacgc ggtagtcagc
     1081 ccatccgctg ttactattga aacagcagaa actaagccga tggacaagca aactacgtct
     1141 cgtagtaaac ctgcgcctgt cgttcgtgcc gcctacgata atgaactggt ggaagccgtt
     1201 aaacgttttc aggcatggca aggattgggg gcagatggtg ctattggccc ggcaacgcgt
     1261 gactggttaa acgtaacgcc cgcccagcgt gctggcgtgt tggcactcaa catccagcga
     1321 ttgcgcttgc tgccaacaga gctttctacc gggatcatgg ttaacattcc agcctattcg
     1381 ctggtctact atcagaacgg caatcaggtg ctggattcgc gagtcattgt cggtcgcccc
     1441 gatcgcaaaa cgccgatgat gagcagtgca cttaacaacg tagtggtaaa cccgccgtgg
     1501 aacgtaccac caactctggc acgcaaagat attctgccga aagtgcgcaa cgatccggga
     1561 tatctcgaaa gccatggcta tacggtgatg cgcggctgga acagcagaga agcgattgac
     1621 ccatggcagg ttgactggtc tacaatcacg gcctcgaatt taccgttccg cttccagcag
     1681 gctccaggcc cacggaactc gctggggcgc tataaattca atatgccgag ttcagaggcc
     1741 atttatttgc atgacacgcc gaaccacaat ctgttcaagc gtgatacacg cgcattgagc
     1801 tcaggctgtg tacgagtgaa taaagcttcc gatctggcga atatgctgtt gcaggatgca
     1861 ggctggaatg acaaacgtat ttctgatgcg ctgaagcagg gcgatacgcg ttacgtcaat
     1921 attcggcagt cgattccggt gaatctctac tatctgacgg cctttgttgg tgcagatggt
     1981 cgtacccagt atcgtacaga tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa
     2041 attgtatcga aagcggaaca attaatcagg taaatgaagc agttcgggga aatcagttgt
     2101 cgtaataatt agcatgattg ggggcaattc tctgcagccc ccgtcactgc tggggttgag
     2161 tcatcttgac gtctgcttta cgggcggtta aggtgcctct tgtgcgccag aagtgcatat
     2221 aaacgataac attgacctgt agacttgatt atcatggaca aattcgacgc taatcgccgc
     2281 aaattgctgg cgcttggtgg cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt
     2341 gcaacactct ctaccccacg cccgcgcatt ttgacactca ataatcttca taccggagag
     2401 tcaatcaaag cggagttttt cgatggcaga ggctatattc aggaagaatt ggcaaaactt
     2461 aaccattttt tccgcgatta ccgcgcgaac aaaataaagt ccatcgaccc aggattattc
     2521 gaccagttgt atcgcctgca agggttgtta ggtacccgca aaccggtgca actcatttcc
     2581 ggttatcgtt ccattgatac caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag
     2641 aaaagctatc acacgaaagg gcaggcgatg gatttccata ttgaaggtat cgcgttaagc
     2701 aatattcgca aagccgcgtt atctatgcgc gcaggtggtg taggatatta cccacgtagt
     2761 aactttgtgc atattgatac cgggccagca cggcactggt agcaattgct taacgaaaca
     2821 ggggcagtat gaactatcgt attattccgg tcaccgcatt ctcccagaac tgttcattga
     2881 tctggtgtga acaaacccgc ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa
     2941 tcaaacagga agttgacgcc agcggcctga cactgatgca gatcctgctg acgcatggtc
     3001 atctggacca cgttggtgca gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg
     3061 gcccggaaaa agaagatgag ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg
     3121 gtctggaaga gtgccagccg ctgacgccgg atcgttggct gaacgaaggc gataccatca
     3181 gcatagggaa tgtggcttta caggtgttac attgccctgg gcatacgccg ggtcatgtcg
     3241 tgttttttga tgatcgggca aagctgctga tttctggcga cgttattttc aaaggcggag
     3301 tagggcgcag tgacttcccg cgtggcgatc ataatcaact gatttcttca atcaaagata
     3361 agttgctgcc actgggggat gacgtgacat ttattccggg tcacggacca ttatccacac
     3421 ttggttatga gcgcctgcat aatcccttcc tgcaagacga aatgcccgtc tggtaaaggc
     3481 gcataaaaaa gcccgctttt taagcgggct tcattgtttt taacgcttac agcactgcca
     3541 caatcgcttc gcacagcgga gccatattat ctggtgtcat cccggccacg tttacgcgac
     3601 cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt tcttttgtca
     3661 ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg cgatttgcgc
     3721 ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc tggcgcatat
     3781 cagtcagttc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg gtggcaacaa
     3841 cagaagcgcc gtgtgctggt gggttagagt agttagcgcg aatcgccgct ttcatttggc
     3901 tgaatgcgcg atcaatggtt tcactgtcag cagcaaccag agtacaagcg ccaacacgct
     3961 cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctct ttatgcatag
     4021 ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaac ccctggtaag
     4081 cgaagtcaaa cagcggtaac cagcctttct caacggaaag ttgtgccagt gtctgccatt
     4141 gttccagcgt agggtcgata ccggttgggt tatggcagca gccatggaac agcaccacgt
     4201 cgccagcctg agcttcgttc aggctgttaa tcagtgcatc gaagtcaagg gtgtgatttt
     4261 ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag acgctcttat
     4321 ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt tttgccagga
     4381 aatcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg cgagcacgtt
     4441 tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag cgaccaaatt
     4501 cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc agatactgtt
     4561 cagccttttt cacactggtc agtaccgggg ttttgcccgt ctcatcttta tagacaccaa
     4621 tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc aggcccagaa
     4681 tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat tatggttaca
     4741 gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta ttactgatga
     4801 aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa aaaacaggac
     4861 caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg gtaaacgata
     4921 cccacagcaa cggtgtcgtc agaacctacg cccagtttgt tgtcagaatc gatctggttg
     4981 atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc gcccacttca
     5041 aagtagttaa ccagatcaac atcaccgata ccttctacgt ctttcgcttt agatttggtg
     5101 taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa cagaacgtct
     5161 tgcgttttgt tggcgaagcc gctgatgttt gtaaatttat tagtgatcgg cgtagcgtta
     5221 cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcatactt cagaccagta
     5281 gcccactgtt cagctttttt accgttgcca agaggttgag cttcttgcag gttggtacgg
     5341 tcagcggcgc cataagcacc aacgatacca aagccttcgt attcgtagct gatagaaccg
     5401 ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct cgtttttacc caggtactga
     5461 acagcgaagt tcaggccatc aaccagacca aagaagttgg agttacgata ggtagcaacg
     5521 ccgccaacac gaccaacgaa gaagtcatcg ctgtatgccg tgtcaccgcc aaactcaggc
     5581 agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc gtaatcgaaa
     5641 gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt accagtttga
     5701 gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc ataaccggtc
     5761 agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt catgtcacca
     5821 ttgccaccgt aactgttttc accgttaccc ttggagaaat aatgcagacc gacagcttta
     5881 ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt tgcagtacct
     5941 gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat tatttattac
     6001 cctcatggtt tttttatgac acctgccact gccgtcaata agttctgtca ataaaaattt
     6061 acggaactat tgatgagagt ttggtgtctt tatgtgtctg caggcatctt tccattcaaa
     6121 ctaacgtttc gctaccgtga aagtgctaca aagataaaga tttggtttca aaaagaaaaa
     6181 atatgtaacc aaaagtaaaa tttaaggaac tttgtgaaca tcgtcatat
//
LOCUS       DAZGAP010000246         6088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGAP010000246 DAZGAP010000000
VERSION     DAZGAP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.247"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..188
                     /locus_tag="ACHLAM_004728"
     CDS             3..188
                     /locus_tag="ACHLAM_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856139.1"
                     /translation="MSRKYSSAMITLVILFCRRPHCRKISVQQYYQKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            149..268
                     /locus_tag="ACHLAM_004729"
     CDS             149..268
                     /locus_tag="ACHLAM_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO7856140.1"
                     /translation="MPLSYAGYHYFIMMSLVSGEHNTLALPIQEQSPGLVPAG"
     gene            303..647
                     /locus_tag="ACHLAM_004730"
                     /pseudo
     CDS             303..647
                     /locus_tag="ACHLAM_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1012..1278)
                     /gene="minE"
                     /locus_tag="ACHLAM_004731"
     CDS             complement(1012..1278)
                     /gene="minE"
                     /locus_tag="ACHLAM_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO7856141.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1282..2094)
                     /gene="minD"
                     /locus_tag="ACHLAM_004732"
     CDS             complement(1282..2094)
                     /gene="minD"
                     /locus_tag="ACHLAM_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO7856142.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2118..2813)
                     /gene="minC"
                     /locus_tag="ACHLAM_004733"
     CDS             complement(2118..2813)
                     /gene="minC"
                     /locus_tag="ACHLAM_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO7856143.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3332..3700
                     /gene="ycgJ"
                     /locus_tag="ACHLAM_004734"
     CDS             3332..3700
                     /gene="ycgJ"
                     /locus_tag="ACHLAM_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO7856144.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3803..4204)
                     /gene="pliG"
                     /locus_tag="ACHLAM_004735"
     CDS             complement(3803..4204)
                     /gene="pliG"
                     /locus_tag="ACHLAM_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO7856145.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4446..4739
                     /locus_tag="ACHLAM_004736"
     CDS             4446..4739
                     /locus_tag="ACHLAM_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO7856146.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4811..5470
                     /gene="ycgM"
                     /locus_tag="ACHLAM_004737"
     CDS             4811..5470
                     /gene="ycgM"
                     /locus_tag="ACHLAM_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO7856147.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5562..6008
                     /gene="ycgN"
                     /locus_tag="ACHLAM_004738"
     CDS             5562..6008
                     /gene="ycgN"
                     /locus_tag="ACHLAM_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO7856148.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         1563 a         1354 c         1495 g         1676 t
ORIGIN      
        1 atgtgagcag aaaatattca tcagcaatga ttacattagt cattttattt tgtcgacggc
       61 ctcattgtcg aaaaataagc gtacaacagt attatcagaa aagagtgatt ttttatccaa
      121 ctacacttca gcgcactgcg tttaaaaaat gcctctttct tatgcgggat atcattattt
      181 catcatgatg tctttggtga gcggtgaaca caatacactt gcgctgccta ttcaggagca
      241 atcccctggt ctggtgcctg cgggatgatg ttgcagcaga gctaaggtca cttagacgct
      301 actatcattt atccaatggc atggggtcga aatcagtcga tacccgcagt atatatcgtg
      361 aacttggtgc aacgctgagt tacaacatgc gtctggggaa cggcatggaa gttgagccgt
      421 ggctgaaggc ggctgtgcgc aaagaatttg tcgatgataa ccgggtgaaa gtgaataatg
      481 acggtaattt tgtcaatgat ttgtcgggca gacgtggaat atatcaggca ggtattaaag
      541 cctcattcag cagttcgtta agcgggcatc tcggggtggg gtatagccat ggtgccggtg
      601 tggaatcccc atggaacgcg gtggctggtg cgaactggtc gttctgacca tcaacgatta
      661 aactgcgctt cgcgcagttt tcgtttaccg ggtgctaaaa gggaaaattc tgcggcaaaa
      721 aaagcccgcc agttacggcg ggaaacctca tcctatggga gaacaatgaa taatgaaatt
      781 gcggggttat catctcccag tatacccata ctaacaataa ggttatttac tcagccaggt
      841 ataaacaatt tgttctgtgt ggggggaacg gcattaagat gtaaaggggg ggaagcaatg
      901 agtagcatca gcaagaatac tcgccgctct tatcaaacgt ggatgagggg gatgaaaaac
      961 tcaaggcaga gatgactctg ccttgaagat aaatgcgctt ttacagcggg cttatttcag
     1021 ctcttctgct tccggtaagg tcacgttcag ctcaagaata gaaatatcgc catctttttg
     1081 ctcaagctgt acggttacca tctcaggatc aatttgtacg tatttacaaa tgacctcaag
     1141 aatatcttta cgcaactgcg gcagataatg cggttctgca tcactgcgac ggcgttcagc
     1201 aacaataatc tgcagccgtt cttttgcaat gttggccgtg tttttcttcc gcgatagaaa
     1261 gaaatcgagt aatgccataa cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc
     1321 ttcttcaatg aagcggaaag gacgttcttc tcccaacaga cgttctacgg tatctgcgta
     1381 ggctttaccc gcatcggcgt taatgtcgag aatgaccggt tcaccctggt tagaggcgcg
     1441 caatactgat tgatcctcag ggatcacgcc gacgagtttg atgcgcagga tctccagcac
     1501 atcttccatg ctcagcatgt cacctctgct tacgcggcct gggttatagc gcgttaacag
     1561 caggtgctct ttaataggct cttcgccatt ttctgcgcgg cgtgatttcg acgccagaat
     1621 gcctaaaata cggtcagagt cgcgtactga ggagacttcc gggttggtgg taataatggc
     1681 ttcgtctgca aaatagagtg ccattagcgc accggtctca atccctgccg gggagtcgca
     1741 aacaataaat tcaaagtcca tcgctttcag atcatcaaga actttggcga ccccttcacg
     1801 ggtgagggca tctttatcgc gagtttgcga tgccggaaga atatagagat tttcagtacg
     1861 cttatcttta attaacgcct gatttagcgt tgcatcgccc tgaatgacgt tgacgaaatc
     1921 gtaaacgacc cgacgttcac aacccataat caggtcgaga ttacgcaggc cgatatcaaa
     1981 atctatcacg acagttttct ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga
     2041 ggttgtctta ccaacacccc ctttgcccga agtaacaaca ataatgcgtg ccatagaaat
     2101 tccttgttaa aaagggatca atttaacggt tgaacggtca aagcgttttc gactaactgc
     2161 agtcgcgccg ctttgccata aaattctgct gggatttgat cactcagcca gtattcacct
     2221 gcgatggaca ccagttccgc catcaggttc gtacaaaata tttgcgtttc ccggtcacca
     2281 cttgcccctg ccagcgcacg accgcgcatc atgccataga cgtgaatatt accatcggca
     2341 atcaattcgg ccccagcgct aacgtggctt gtaacaatca gatcacattg tggagcataa
     2401 atacgctgac cggaacgcac cggggtatct attaaacgcg tttttgtgac cggcgttgta
     2461 ttttgcgctg gagcctgcgg tgcgggagct ggacgcggcg ctttttcctt tccttccgtc
     2521 aggataggca gccccatctt ttcaatttcg gctttaagtt gcgcatcttt gcagccactt
     2581 acgccaataa cccgcaaacc ggttgccgaa accgccttat gcatcgctga ccagtttacc
     2641 gggtcttcca gtgcactgac gttgagtaca acgggggcat gttttaaaaa tgcgggggcc
     2701 tgagcgattt tgtcttccag cgcctgatgg ataaccttag gttctgcctc atgcagatga
     2761 accacagata aagtgaagct actgccttta agctcgattg gcgtgtttga catcctggcc
     2821 ttactcaatt agctattaat catcgccagc gcgcgatgat gttccgaaga ctataaggca
     2881 tgttatagtc tggattatat tgaggcaagt caccctccca tttattcaga gtaaaagtct
     2941 attctgtgat aaatggcgct gattcatagc ttaaaaaata cccttgtcaa tcaacccatt
     3001 gccgtcgtac ttttgattgt tcttatttac gcttcctttt tccgcaccct aatcgtgaca
     3061 gcatcttcag acctgtttaa atatccagta ttgaggcgag tgtatttttt caccacgcca
     3121 ggctttctta actttccatt tttgcagcat cattaaagaa cagatacccg ttatcttaat
     3181 atccgccaca gaattgctct atcatagaaa ccgcagaaga cgtctgatac ttctgtcagg
     3241 tcatcgtaac tcgtccaggt cagcggtttt gattacggat tgatgatgaa atgagcataa
     3301 gtgttgtacg gttggattat caggatgtgc tatgaatatg atgcgtattt tttatatcgg
     3361 attgtcaggt gtggggatga tgttctcatc aatggcgtct ggacatgacg ctggcggact
     3421 tcaatctccg gcgtgcggcg ttgtttgcga cccgtatatt tgtgttaact cagatggcat
     3481 ttccccagag ttaacaagga aatatctcgg cgaaaaagcc gctgaaaact tacaatcatt
     3541 acaaggctac gatcccagcg aatttacatt cgctaacggt gtattttgcg atgttaaaga
     3601 aaaattatgt cgtgatgatc gttattttgg tgtgaatggt aagcggagtg gaaaaatcaa
     3661 tcaaaccacc acaaagatgt tatttatgtg tcgtgagtga tgtcttgttt acattatgtt
     3721 ctattcgtca ttaacctctt gagcgacaga gaggagtggg gcgttaacgt ttatgacggg
     3781 gagagtcccc gtcggttgat atttacttaa tttgaatatc gacgttatat tttttagttt
     3841 tgtttttacg ggcgtcgttt cgggtttgca gcactcgcaa ctcatatttg ccagaagcag
     3901 ggagtgagta ttgtccgtga ctatcgagtt ctggggaata tctggataaa tcaacagaat
     3961 catcgattcc tggcccgaag aggtaagtat cagcgccttc attagagata ctcacatgca
     4021 ctttctgacc ttttttggca tagaaggtat atgtatcgta atcgtatccc tttatttcgc
     4081 cggagtactg agcgctgcta tggccttttc tgaactcaac attgacattc ttacccgcag
     4141 caaagctcgt ggaggttagc agtgcaagca acaatacagc cttactgatg cccttaattt
     4201 tcattggtag tctcctgttc agaatgagta cgatgaactg ttataatata acaattcatc
     4261 acaggagaat tccagtggct ttcctgcgtt cggttaaatc ccggcgcggc atttttgccg
     4321 gggggattac ttataaaggg ggcatggact gaacaaaccg taggaatcta cagctggctg
     4381 gcttgtggcg cgggtttcat tgatgttagc ttatgccaaa acctggcatc ttaaagagta
     4441 aaagtatgtt ttgtgtgatt tatcgaagca gcaagcgtga tcagacctat ttatatgtcg
     4501 aaaaaaaaga cgatttttcg cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc
     4561 agttagcgat gattcttccg ctggatgggc ggaagaaact ggtcaatgcc gatattgaaa
     4621 aggttaaaca ggcattaacc gagcaaggtt actatttgca gttaccgcca ccacccgaag
     4681 atttgctgaa gcaacatctt tccgtcatgg ggcagaaaac agacgacacg aacaaataac
     4741 cgatatccgg cggtggcatt atctttgtcg gcgcgggttt tcatatccac gataaggtga
     4801 ggggaacgtt atgtatcaac atcacaactg gcaaggtgcg ctgctggatt atccggtgag
     4861 taaagtagtc tgtgttggta gtaactatgc caaacatatt aaagagatgg gcagcgcagt
     4921 gcccgaagag ccagtgctgt ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc
     4981 attggcgatc ccatccgatt tcggttcagt tcatcatgaa gtcgaactgg cggtattgat
     5041 tggcgcgacg ctgcgtcagg ctacggaaga gcatgtccgt aaagccattg ccggttacgg
     5101 cgtggcgctc gatctgacat tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc
     5161 gtgggaaaag gccaaagcgt ttgataactc ttgtccgctt tccgggttta ttcccgcggc
     5221 ggaattcacc ggcgatccgc aaaatacaac gctgggcctg agcgtaaacg gcgaacaacg
     5281 ccagcaaggt acgactgcgg acatgatcca taaaatcgtt ccgctgatcg cttatatgag
     5341 caagttcttt accctcaagg ccggtgacgt tgtgctgaca ggcacgcctg atggcgtcgg
     5401 cccgttgcaa agtggtgatg agctgacagt cactttcgat gggcattctt tgacaactcg
     5461 cgttttgtaa tactttttgc cgcctgaaag cggcggcaaa acttgcatcg ctgtgccaga
     5521 ctggttataa ggtgcgtttt aacgtaatgg cggaacacct gatgagcgat gtacctttct
     5581 ggcaaagtaa aaccctggac gaaatgagcg atgcggaatg ggagtcgttg tgtgatggtt
     5641 gcggtcagtg ttgcctgcat aagctgatgg atgaagacac cgacgaaatc tacttcacta
     5701 acgtcgcctg tcgccagctc aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt
     5761 tcgagtttga acctgactgc attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc
     5821 tgccaatgac ctgcgcttat cgcttgctgg cggaaggtaa agatttacct gcgtggcatc
     5881 cgctgcttac tggttcgaaa gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg
     5941 cagtgaaaga atcagaagtg attgactggc aggatcatat cttaaataaa cctgactggg
     6001 cgcagtgata aatttaatag atgttgatgg gggctttctt aatatacgtc tggatactcg
     6061 ccatttcgta gccctgtttt tgaaagcg
//
LOCUS       DAZGAP010000247         6062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGAP010000247 DAZGAP010000000
VERSION     DAZGAP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.248"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..343
                     /locus_tag="ACHLAM_004739"
                     /pseudo
     CDS             87..343
                     /locus_tag="ACHLAM_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(365..636)
                     /locus_tag="ACHLAM_004740"
                     /pseudo
     CDS             complement(365..636)
                     /locus_tag="ACHLAM_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(633..2393)
                     /locus_tag="ACHLAM_004741"
     CDS             complement(633..2393)
                     /locus_tag="ACHLAM_004741"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO7856149.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPRITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPSADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSSVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            complement(2386..3027)
                     /locus_tag="ACHLAM_004742"
     CDS             complement(2386..3027)
                     /locus_tag="ACHLAM_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO7856150.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            complement(3031..3948)
                     /locus_tag="ACHLAM_004743"
     CDS             complement(3031..3948)
                     /locus_tag="ACHLAM_004743"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7856151.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPSKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            complement(3945..4550)
                     /locus_tag="ACHLAM_004744"
     CDS             complement(3945..4550)
                     /locus_tag="ACHLAM_004744"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7856152.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            complement(4608..4793)
                     /locus_tag="ACHLAM_004745"
     CDS             complement(4608..4793)
                     /locus_tag="ACHLAM_004745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856153.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            complement(5034..5900)
                     /locus_tag="ACHLAM_004746"
     CDS             complement(5034..5900)
                     /locus_tag="ACHLAM_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7856154.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFLWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSLESV
                     FILILSVILFSVKINSPLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLS
                     ASFYVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPIESVFLVFLSGI
                     LQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITIL
                     CVYILNKKYAIE"
BASE COUNT         1702 a         1570 c         1431 g         1359 t
ORIGIN      
        1 acagtctatc tctgcctttg cttcatggga tgttctttgt atattttact tgctgtttaa
       61 gtatgtatat aaggagtgca ttcattatgc caatgaagct tgtaaagaag aatccccgga
      121 gcaaagtgat tcgggagctt agctgtgagc aagagatggc agtaaaggcg gtctgcggac
      181 tgaaaaaaca gcaactacgg cactacatgc cttggccaat gatcttctcc gggaaatgcg
      241 tgagtatgat gtatggttac agtgtgactg tattccggga gattcgccga ccatgaattt
      301 tgctgctctt aaaaacaaca cgggaacttt ttatctgtcc agtttggata tagataatta
      361 ttgtactaat aagggaagcc aacaccagcc gacagaccag acgccgctgc tcattcagtg
      421 aaacgcagat aaacagtaaa gcaaatccag actccagtac ggaataatcg tcagcggtat
      481 taacgggatc cggtgctccc agtcaaacac caggctgctg atgttttctc tcccggcggt
      541 aaactgattc gtcatgccat aagtaagaaa gaagaatggg gccagcacca ttagccagcc
      601 gattccctga ataatcaccg cacgccgcgt tgtcatggtt gacgtcgtac cgccatagag
      661 accgtgaaga tgcccatttc atcaattagt tgcgtgcatt tctcaaaacc ggcgtcacgg
      721 accagcgcgt ccatctcgcc ctgagtacgt acgcgcatta cccaggcttt gccatttttg
      781 tgactggtga gtgaccaggc gatggttttt aactgagggt gccagggctg acaggtgtag
      841 atcagaatgc ctcccggttc aatcgctgca gccagcccgg caagcgaatc tcgtaccggc
      901 ccgttttccg ggaacagttc atacagaccg gacacgatcc ccagcgtcgg acgcggttcc
      961 agtgccgcca gttcattacg attaaaggca tccccctgct caaatcgcgc acggcccgcc
     1021 ataccgcgag aggctatcat ctcctgacct ttcgccacgt ttaattcgct gtaatcgcgc
     1081 agaaggatct cgcttaccgc cgtttcgcct tgcagggcat caagcacata gcgtccatgc
     1141 ccggcggcaa tatccacgat ccggaccgag ctaccctgct ctgttaaccg agcaacagcc
     1201 tgctgaatta acatctgcag atgaatttta cgctggcgga tccccttcca gcccacgcta
     1261 ttcaggtagt ttttatcaat gaggcgtccc aacgaggtgc tgccctgagg ctggttgcga
     1321 tagatgtagt ctagggtact gccggaatcg aaaccagtct caaatcccag acgcaccccc
     1381 gtggactgcg tgcccagcac tttcatcccg tagcgcaaac agcggtaaga gatatcgtcc
     1441 agcgaataca gcgcagggcc accgctcagt atgcgccagg tatcggcgct ggggctccag
     1501 cgatcttcct ggctgtaatc aaaacgctgc acgggacggg catacaaggt atcaataaaa
     1561 ctgcgcatcc tgtcaaatgc cagatgccgc tctttttcgc ctaaggtgtc atgataaaat
     1621 cccggcagta catgcagttc tttctgcgga tttcgcaggt tctgataaaa atcaagctgc
     1681 ggctggcggt gcacaacgta atcttccccg gagaccagca actgcacagg tagcgtaatg
     1741 gcgctggcat cgctgacgac tcgctcagcc gttttgtaga gatcaagcag aatattcaca
     1801 gcaattggtc gggtaatccg cggatcctta ttgaagcttg ctacccgctc gggatcgtgc
     1861 gtcagatact tgcctttcac atacgagttg acgaagaaaa gaccgcgcag gcgatacatc
     1921 aattccagcc cctggcgagc aaacggcaca tacaatttga ccttaaacgc cggtgatgcc
     1981 aacaccaggc cacgaatgtc cggggcataa tcatgcgccc aggttgccgc cagaaccgcc
     2041 ccgacgcttt gcgccacgac gaccacatcc gtcatcccag cttggctatc tgcggcggcg
     2101 aaacgcacaa actcgtcaac atcctgcacc gaacgcacga gcgacggact gtaaccacgc
     2161 agacccggtg agcgaccatg acctcgggca tccatggcat aaaacgctgt atccggcatc
     2221 gccagctcat caacaatgtg ttgcaaacgc ccggagtgct catgcccacg atgaaacagg
     2281 accaccactt ttggcgttga actggcttct tgtgctggcc agtggcgata aaacagctct
     2341 gtattatcac tggtcagaaa ctgccgttca tcgggtatgc gggaattagt catggggatg
     2401 atttccttgc gagggttgca aaagcgccgt tagtctttgc tccaggtcgt ccataaacgt
     2461 tttcttctcc ggatgcgtac ttagcgcatc gccaatattt acatcaataa acagcggcaa
     2521 agggatacga ttacctttcg ccatcacacg ctcagtaccg cgcaacccaa cggggataat
     2581 gggcacctcc ggcatgcgct gactcaaatg ccacactccg gatttcaatg gcgcaagtac
     2641 gcctggctca ccgcgcgtcc cttccggaaa caggatcagt gttttacctt cacgcagcgc
     2701 ctgttcacat tgcgccagcg gatcggtata accgcccttt cttgaaatag gaatgatgtt
     2761 aaggatcttc agcgcaaacc aagccatgcg tttatcccgc agaaaataat ccgcggcagc
     2821 aaccggatga acagaagcct ggtaacgcag cggaaaaagc gaaagcaagg cgaaaacatc
     2881 catatggctg ttgtgatttg ccacgattat cgaaggcccg tgcttcggca gtcgctcgcg
     2941 gtggcggaca cgcagaccaa gccacagcca gatgaccggc cagacgatac acagcgtaaa
     3001 cagggtacgg agtagtcgtg tcatcatagg ttagtagcag aaatagcgaa caaaatggaa
     3061 gaagaccggt gcggtaaaaa tcagcgaatc cagccgatca aggatgcctc catggccggg
     3121 tagcagcgta ccactgtctt tgacgccgat atcacgcttg attgccgaca tcaccacgtc
     3181 gccgcaaaag ccagtaacgc cgataataac cccggctaac agtgccatcg gccagctcag
     3241 gggagtcaac agtggcccca ggactgttgc cgccagcgcc gtcgttgcca ccccacccag
     3301 caatccctcc agggttttgt ttgggctgac cttcggtatg acttttatac gaccaagcga
     3361 cttgccccac aggtattgcg cgatgtcgtt aaattcggtc aggccgacca aaaacagcac
     3421 caacagcgcg ctggtttgca ttccatccgc cggtaacacc agtaaaaacg cgacatggct
     3481 gaaggcaaat acggtggtca tcagactcca gtgcaactgg gaaacggtac gtaaaaagcc
     3541 ctgtgtatcc ccagcaatca ccattcgcgc cggtaaaaaa aggaacacgt agaccgggat
     3601 aaacaccaca aacatgccgt accagccgat acctatcagc cagtagttaa ggggaattgc
     3661 aataaacatc cataacagcg gcatgcggtc ggactggcgg gaaggcacca gcgtcaaaaa
     3721 ctcttttagc gccataaagc tgaccagcgc gaagaacgtc agtgccagcc agctaggact
     3781 taatagtgcg aaggaaaaca ggacgataat cacccaccag gtgcgaatac gtaatgtcag
     3841 ctcgcgccag tctttacttg gacgcagcca caccagcaac ccgttgatta cggtggcaat
     3901 caacagcagg gaaaaaataa cgatcagtga tttttcaagt agcgtcattt cacaccctct
     3961 tcacgcaatg cgctgcgaca gcgattaaaa atcgtccata acaacaacag tgttgcgaca
     4021 ccccatatga cattattcca ggcgacaatc tgcggccaga tcgacagtga cagcccccat
     4081 gcaccaaaca ccagcgctct gtcgcttttg cccagcggac cggcatagct gcgcacgccg
     4141 ttaatcgttt gcgccagcac gccgcaaaac tcggtcatca gcgcacaaaa caccacgatc
     4201 agcaccagcg gtatactgct ctgcgggagt agcataaagg gcagataaag cgagatgtcg
     4261 gaaattacat cacccgtctc attgaggatg gcccccagac gcgatctttg attacattca
     4321 cgcgccagca taccatcgag cgcattgagc gccattcgta caaacaacac cacgggcaga
     4381 aggacgaata atccgggctg aggatgaata atgagcacca cgccggtgat aagggaaagc
     4441 accatcgcgc acagtgtaat gtgattagcg gtgacgccgc gcctgttcag ccagaacatc
     4501 agcggtctta acagagcctg aaacgagggc tttatctggt aaagggtcat catcactcct
     4561 taataagcat atttgacggg gttgagtctg gccttttatt ggggctgtca ctggcacact
     4621 gaaagtgaat acagttccgg gcgcggaaat cctgcctgct gcgccggtaa aggcgactga
     4681 ctggtattac gggaggccag agagccagta cctgtgccgg tagactgtgt ggctgaacca
     4741 taaacatagt gtcctggaca acggtacagc actctgtgca gctgtgtcag catgcgcttt
     4801 tctttaatta tgcccagtca tgtgcggagg caaggatttc aaaatatccg ttacatttgt
     4861 tgcatgatgc cttatcgctg gtccgggcta ttgctttact tcttttaaat cagctctgat
     4921 gaacattttt taccacttta tcgcattaag gttatgtcat tcatcgtctg gcatcacgtt
     4981 tttggttatg gtgtcagggt gtaccctacc gcgataacgc aaccctaata aagtcattcg
     5041 atggcatatt tcttattgag aatatatacg cataaaatag ttataaaaac gcctagtccc
     5101 tgaataaatg atatatcttc ctgcaggaag aaccaggaca acagcaatgc taccagagga
     5161 ataaaataga gcattgctcc tgctatgtga acgggaatcc agcgtaggga ataaagatag
     5221 aaacaaaaag ccaaaaaata ttgtaaaatt cctgacaaaa acacaagaaa tacagattct
     5281 attggcagtt gcagggatgt gccagttaga acaataaaaa aacaagcaag aataaaagag
     5341 aaagtcaact gcccggttaa caacgtaaca ggctcaaaat tcacaataag tcgtgatgag
     5401 ataacaacat aaaaggatgc tgagagtaca cctgcaacaa taagtatata tcctgtaata
     5461 ttatcaatgc ggtcattgat atcaggtagg gcaaccatta tactgcctgc cattgctcca
     5521 gtaagcaata atataaagtg taatggtgag ttaattttaa cggaaaataa tataacagat
     5581 agtataagaa taaatactga ctcaagtgaa aaaataacag ctgttatacc agcagaaata
     5641 aattttaggc catagagagt gaatgtatac gctagaaatg gctcaaaaat tcctgttgaa
     5701 taggcaagga ttgatttttt tgttaccggg agtttttttc tgctaattgc gagaacaaac
     5761 catgataaca ttgcagcact accaacttgc cataaaaaca actctccggc atccatgttt
     5821 tcgagacctt ttttgctcat cactattgct gctccccatg aaaaagcagc aagaaatgca
     5881 aacataattc ccttaatcat atttagttat ctcctcagta taaagaggtt tattttcaaa
     5941 cgctcttggt ggaaatggat taaaaaactt tgcttcttca tccacaggaa tacctggaat
     6001 ttctccataa taaggattgc caacaggaaa cttttccagt gagacataaa acaatacatt
     6061 cc
//
LOCUS       DAZGAP010000248         5981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGAP010000248 DAZGAP010000000
VERSION     DAZGAP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.249"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..236)
                     /locus_tag="ACHLAM_004747"
     CDS             complement(<1..236)
                     /locus_tag="ACHLAM_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HFO7856155.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKV"
     gene            complement(304..1785)
                     /locus_tag="ACHLAM_004748"
     CDS             complement(304..1785)
                     /locus_tag="ACHLAM_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO7856156.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESRIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            complement(1778..3268)
                     /locus_tag="ACHLAM_004749"
     CDS             complement(1778..3268)
                     /locus_tag="ACHLAM_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO7856157.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVILVGDR
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEGYRGTGYDISGLVQENGHA"
     gene            3500..5020
                     /locus_tag="ACHLAM_004750"
     CDS             3500..5020
                     /locus_tag="ACHLAM_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7856158.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAGDTATLA
                     AARTHSDENNKRTLISANTYADQKETAINNRTDGLLMTERQARIAGDTATLAAARTHS
                     DENNKRTLVSANTYADQKETDINNRTDGLLMTERQARIAGDAETLATAEAYSDTGDSL
                     TLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPYV
                     AENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
     gene            complement(5260..5748)
                     /locus_tag="ACHLAM_004751"
     CDS             complement(5260..5748)
                     /locus_tag="ACHLAM_004751"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7856159.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHNIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
     gene            complement(5754..>5981)
                     /locus_tag="ACHLAM_004752"
     CDS             complement(5754..>5981)
                     /locus_tag="ACHLAM_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO7856160.1"
                     /translation="HAMLKRAAEIQGRTMTDFIISAVQDAAQRAIEQTDIIRLSRTDQ
                     EIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
BASE COUNT         1576 a         1699 c         1319 g         1387 t
ORIGIN      
        1 accttgttat tccaggtcac ctccctgcgc ttctgctctt tcaggacata acgccggtat
       61 cgctgaacat cacggacagt cacctccccc ggcaaaatat caccgataaa ttccctgaat
      121 accctgacaa ctttgcagta gctccactct gtatccggcc gaagattctt tgaaaaaaag
      181 tactcttcca gcagttgttc ccaggtgatt tttgtgacgg aatcgtcatt ctgcattata
      241 tattcccctt gtttactgtg ctcccgcact ctccggtcaa attctgcaca tcatcgcctt
      301 tactcacccg aaaacaataa gcgggctgtc tccgggaacc gtaatattcc cgtacagaaa
      361 cctgccctta accagatatc ccgaaaccat tttgtaacgt ccggaatgtt ctgcgtcact
      421 ataaagacgg gcctgaaaaa aacgctctcc tgaacgtacc ttatggatac ctgcccgttc
      481 aaagagcgac tgaacataac gacgatcctg cccggaacca ctctccttca gaaaacgata
      541 aaaaatcccc ggggcacaca gaaaaacata tccggaaaca acatgaacat ttgaatcaac
      601 gctgttaacc gaaagtgaac cctccctcag cccctctttt aaccatctga taaattcact
      661 ctctaccctg ctatcctctg tcagggaaaa ctctgccgtt ttttccactg tttccgggat
      721 acggtttccc tgattttctg ttacacatcc gctctcttcc ggtaactcaa tacggctttc
      781 tttatccggc ggcacgcctg acgctatctg ccctccctca tccgacaaat cagcagccga
      841 aaacagcgta aggagcattc cggtatcagc atcagtttcg ttcccctcat ccacctcctg
      901 ttccgctgtc cggccctcat tttcggcatc ctccactggc gactgtacaa ccggcaacgg
      961 ctgaatgacg ctttctgctg aaattgctgc tgactgcagc agtaccggaa caacctcagc
     1021 caccggaact tcggccacat ctcctgcagc gaggggactc acgacttcct ccatagcttc
     1081 ctgtaatact gtacgtatca gcgccatttc aggatgctga tgccagactg ctccggcaag
     1141 attctgtaat gcccccggga cagtggcaag ccaggctccc gcctctccgg gcagcagttg
     1201 tcccgccatc agggctgcca gttgccgtcc ctccgttccc tggcagggca tcttcctgaa
     1261 ccggaaccgg tatacgccct ccggtacaga tatccccggc tgccagcatc tgccgtcggc
     1321 ctgttcgcct tccagttccg ccagcaacgg caggtgataa aacagagccg accagaacac
     1381 caccatgttc cacacactcc cctgcgcggc ctgctcttct gctgtcgctc ccggtggaaa
     1441 cattctgcct ctggccagcc ggacagcata cgtcgtgaac tgcagggtca ggtcaccaaa
     1501 accaccggtg taaccccagc gaccttctgc tgtggccggc agattctgac tgacagacaa
     1561 caactgatgc acaggatcaa gacaaaggcg ctgatacata tccaccggca tggaactgtt
     1621 ttcccataac tgtttcagac accttttcct gaacggtgtg gataccagta catcagccgg
     1681 caatggtgta aaatacccct ccggcaccgt ttccccggga atatccgtcc ggacatcaga
     1741 gcattctgtc ttcacaggaa ataacctttt caggcgttca agcatgccca ttctcctgaa
     1801 ccagacctga gatgtcgtac ccggtccccc ggtaaccttc agttttctgc tgttcttctg
     1861 ccagttggtg catcagatca ttcagtacca gcgtcctgag ggcccgggtg acagccacat
     1921 ataacagaca ggtttcatca tgacgctcag cctccgtcat cagcggatcc gtgatatcct
     1981 ggaaatcctc atccagcatt acaaccggcc actccagccc cttgctccgg tgtgccgttg
     2041 acacagtgat atctgcatcc tgctcattcg ttaccgtata ctgccggaga agttccagtt
     2101 ttttcggtaa cggaaaacac aactccagaa gccgaaggga ctgattcatc tccacatcct
     2161 tcgtggattt tgcaatatat ttatattctt caaaattcct gtagtcccgg cgcagcctgt
     2221 ctgaatgcat ccgttccggc atatcaacct ggaaccagta gagatcctca agttccccgg
     2281 tcctgtaacc ttcgattccc cccacccagt aaaccttttg ccctttcatt gccgccatca
     2341 gcgcagtacc aatcacaccc gcgacagtac gactgagtac cgtgtaatgc ccctgcagat
     2401 gttcacgcgc atggcatttc agcaatacct catcatcacc accacaacct ttcacctctc
     2461 gtgtttcacc ttcacgctgc agaagaagat ttgccatgcg agccaccgcc ggaccaaaac
     2521 ggaaactctg tgtcagccac aaccggtccg cattcttcag tgcaggatgg gaaagtgcat
     2581 tattcgcacc ccgaaagcgg taaatctgtt gataacgatc gccaacaagg atcacccgac
     2641 acttctgccc cagaaccaga gcagtggtca ccggattcgc atcctgcgcc tcatcaaaca
     2701 ggatagtgtc ccatcggtga gacaggtctg caccggacaa ctggaagagt ttcagataag
     2761 tatcgtgcgt caccgggaac gtccccccgg ggttgctcat ttcgttccag agcaactgca
     2821 ccgcccgaag cgcatctgct ggcggaacac cccgcatctc atccttatcc ggcaggtgat
     2881 gcaggcccgg taccgcatcg gcagaacaga taaagtgatt cagccccgtc cctgccaccc
     2941 gtgccagagc ccagtatcgt gtattcagct ttctcgccac atcagtaata cgtaatcccg
     3001 gaaccagccg atgacggtag tgtcttccaa cccgggcata cgccagctga tgtgacgttc
     3061 tgcattccac gttaaaggga aatttacgct ctgcctcatc acgtattgcc cggttatagg
     3121 caagatagag catacggctg tccggactgg cccgggcaaa ctgtaccagc gtactcgtct
     3181 tccctgtgcc ggaaaacgca ttcacaacca gttgctgccc ctgccagtta ataatggcgt
     3241 actgttcatc tgtgggatta aaagacatgt attctcttct ccggaaaaac atttccgtaa
     3301 tctgatgcat caggggtatg cccaccacaa ataactctgt acagcaacag aaaaatttcc
     3361 ctggtcatat gatgaattaa tcatcgccat cacaccggat gcttctcatc ctgtcctcag
     3421 aaaggcaggt ggtctcctgt ctgccgcgct tatccaggat accgggacct gaaaggtcat
     3481 aatctggcag gaaaaaataa tgtccgaagt aaaaaaacac gtttcttatt cattaacagt
     3541 tgcagctatg ctgtcagccg ccatcgtctg tcagacagca catgctgatg tcaacacacc
     3601 gaacatcacc ggagaaaaaa acaatacaac aacacgattc tccggaaata tttacggcag
     3661 tgaaaatact gtcacatcag agtcaaacag tctggttgtg ggtaacagta attccgtgga
     3721 gggagggtat aacaacattg ttattggtaa ttcctccacg gtaaaagctt ccggtttgat
     3781 cagaaaagtg ggcgaagata ttgtgctgga tggttacagc gtcagcctcg gtaatgccac
     3841 cagaattaac ggtgattcat ctgttgccat aggtctgaca tcgtccgtaa ccgggaacga
     3901 ctccgtggca ctgggcagtc attccagtgc taacggaaac aatatcgtat ccgtcggctc
     3961 tgacacactg aaacgccgca tcacaaatct ggcacagggg aaagatgacc atgatgcggt
     4021 aaatctgatg cagatgacca gtgctgttaa ccgggaagct ggagaaagaa taaaatcaac
     4081 aaacaacctg cagaatcagc ttaataccct ctctggtcag gtcataactg aaaccagggc
     4141 ccgaacagaa ggtgatgcag cagcgctggc tgcagccaga gcccacagcg atgaaaacga
     4201 caaaagaaca ctggtcagtg cgaataccta tgctgaccag aaagaaaccg ccatcaataa
     4261 ccgtactgac ggattactga cgaccgaacg ccaggcacgc accgcaggcg atactgcaac
     4321 tctggctgct gccagaacgc acagcgatga aaacaacaaa agaacactga tcagtgcgaa
     4381 tacctatgct gaccagaaag aaaccgccat caataaccgt actgacggat tactgatgac
     4441 cgaacgccag gcacgcatcg cgggcgatac tgcaactctg gctgctgcca gaacgcacag
     4501 cgatgaaaac aacaaaagaa cactggtcag tgcgaatact tatgctgacc agaaagaaac
     4561 cgacatcaat aaccgtactg acggattact gatgaccgaa cgccaggcac gcatcgcagg
     4621 tgatgcagaa actctggcaa cagcagaggc atacagtgac accggggatt cactgacact
     4681 gaaacaggca acagcctaca gcgatatcag tacccgccgg gcactgtcag aggccaatcg
     4741 ttataccgac aacaaattca gaaacctgag caataaaatt gaacgggcag aaaaaagact
     4801 taatgcaggc atcgccggag tgacggcaat atcctccatc ccctatgtgg ctgaaaattc
     4861 tttttcatac gggatcggcg tgggtaacta tcagaacgct aatgctgtgg ccgcaggcat
     4921 tcagtataaa acatcacgta ataccaatat acgcctcaac atctcctggg actcatctca
     4981 taacaccgcg attggtgcag gggttgccgg cggctggtaa ataatcttgc gtacatacgg
     5041 cattatggta ttccgtaatc acattaattc agtacgcaac acagccccat ccggaacaga
     5101 ccactggact gagcaggata atcaaggaaa aagggcgcgc agtgtggtca gccttcacaa
     5161 caatccggcg gcagatttcc tgccgccgtg ttgcccgtta acatttaact tcgtgggctg
     5221 acgttgccac aagcaccgta tttccctgat ttattctgtt tattttattg tggataatga
     5281 tgccaatggc atgaataaag ttcttccgga ctccgggaac gccataaagc cataatgttt
     5341 gtaaaatgcg actgccgtgt catcttttgc atcaacaata agcgcatatg ctgcaatttc
     5401 agatgtgtat gcacgatata gcgcatcagc taaaagtgct cccccaagcc ccgttccttt
     5461 tgatgacaga cttactgcca gtcgtcccat acgaatagcc ggaatagtgg gatatcgcgg
     5521 aagctttttt ctcaaatgct caggtaagtc agttaataat atactggccg ctgataacgt
     5581 atagtatccg atgatattat gatcgttatc tacagcgaca aaacaggccg cgatgcgtcg
     5641 tcgaatatcc tgtgtgacat gctcttttat gtaacggtcc agagcatcag agccacataa
     5701 aaatgaggtt ctgtcatatg tgctgttaaa tggtgtcagt tgaaacatta tatttatttt
     5761 gtgtacagca tattattacg gcgtttaaag gcccgtttaa gcgcatcgtt cggctctgtt
     5821 ggtgacagta atgcctgagc aaaaatttcc tgatctgtcc gggacagtcg gattatatcg
     5881 gtttgttcga tagcacgttg ggccgcatcc tgaacggcag aaatgataaa atcggtcatt
     5941 gtgcgcccct ggatttctgc agcgcgcttc agcatggcat g
//
LOCUS       DAZGAP010000249         5944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGAP010000249 DAZGAP010000000
VERSION     DAZGAP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.250"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..238
                     /locus_tag="ACHLAM_004753"
     CDS             <1..238
                     /locus_tag="ACHLAM_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856161.1"
                     /translation="FYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELA
                     GTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            318..2465
                     /gene="cadA"
                     /locus_tag="ACHLAM_004754"
     CDS             318..2465
                     /gene="cadA"
                     /locus_tag="ACHLAM_004754"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO7856162.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSSWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYEDMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            2524..3981
                     /gene="dtpC"
                     /locus_tag="ACHLAM_004755"
     CDS             2524..3981
                     /gene="dtpC"
                     /locus_tag="ACHLAM_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO7856163.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            4218..5735
                     /gene="lysS"
                     /locus_tag="ACHLAM_004756"
     CDS             4218..5735
                     /gene="lysS"
                     /locus_tag="ACHLAM_004756"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7856164.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(5854..>5944)
                     /gene="ghoT"
                     /locus_tag="ACHLAM_004757"
     CDS             complement(5854..>5944)
                     /gene="ghoT"
                     /locus_tag="ACHLAM_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO7856165.1"
                     /translation="KTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT         1493 a         1401 c         1442 g         1608 t
ORIGIN      
        1 cttctactct tgcgttgacc tgattcgttt tgaaggcgtt aacatccgca actttgtcag
       61 cctgatctgc tctgtactgg gttgcgtgtt ctgcttcatc gctctgatgg gcgcaagctc
      121 cttcgagctg gctggtacct tcatcgtcag cctgattatc ctgatgttct acgctcgcaa
      181 aatgcacgag cgccagagcc actcaatgga taaccacaca gcatctaacg cacattaatt
      241 aaaagtattt tccgaggctc ctcctttcat tttgtcccat gtgttgggag gggccttttt
      301 tacctggaga tatgactatg aacgttattg caatattgaa tcacatgggt gtttatttta
      361 aagaagaacc catccgtgaa cttcatcgcg cgcttgaacg tctgaacttc cagattgttt
      421 acccgaatga ccgtgacgac ttattaaaac tgatcgaaaa caatgcgcgt ctgtgcggtg
      481 ttatttttga ctgggataaa tataatctcg agctgtgcga agaaattagc aaaatgaacg
      541 agaacctgcc gttgtacgcg ttcgctaata cgtattccac actcgatgta agcctgaacg
      601 atctgcgttt acagattagc ttctttgaat atgcgctggg tgctgctgat gatattgcta
      661 acaagatcaa gcagaccact gacgaatata tcaacactat tctgcctccg ctgaccaaag
      721 cactgtttaa atatgttcgt gaaggtaaat atactttctg tactcctggt cacatgggcg
      781 gtactgcatt ccagaaaagc ccggtaggta gcctgttcta tgatttcttt ggtccgaaca
      841 ccatgaaatc tgatatttcc atttcagtat ctgaattggg ttctctgctg gatcacagcg
      901 gtccacacaa agaagcggaa cagtatatcg ctcgcgtctt taacgcagac cgcagctaca
      961 tggtgaccaa cggtacttcc actgcgaaca aaattgtcgg tatgtactct gctccggcag
     1021 gcagcaccat tctgattgac cgtaactgcc acaaatcgct aacccaccta atgatgatga
     1081 gcgatgttac cccaatctat ttccgcccga cccgtaacgc ttacggtatt cttggtggta
     1141 tcccacagag tgaattccag cacgcaacca ttgctaagcg cgttaaagaa acaccaaacg
     1201 caacctggcc ggtacatgcc gtaattacca actctaccta tgatggtctg ctgtacaaca
     1261 ccgacttcat caagaaaacg ctggatgtga aatccatcca ctttgactcc gcgtgggtgc
     1321 cttacaccaa cttctcgccg atttacgaag gtaaatgcgg tatgagcggt ggccgtgtag
     1381 aagggaaagt gatttacgaa acccagtcca ctcacaaact gctggcggcg ttctctcagg
     1441 cttccatgat ccacgttaaa ggtgacgtaa acgaagaaac ctttaacgaa gcctacatga
     1501 tgcacaccac cacctctccg cactacggta tcgtggcgtc cactgaaacc gctgcggcga
     1561 tgatgaaagg taatgctggt aagcgtctga tcaacggttc cattgaacgt gcgatcaagt
     1621 tccgtaaaga gatcaaacgt ctgagaacgg aatctgatgg ctggttcttt gatgtatggc
     1681 agccggatca tatcgatacc actgaatgct ggccgctgcg ttctgacagc tcctggcacg
     1741 gcttcaagaa catcgacaat gagcacatgt atcttgaccc gatcaaagtc accctgctga
     1801 ccccggggat ggaaaaagac ggcaccatga gcgactttgg tattccggcc agcatcgtgg
     1861 cgaaatacct cgacgaacat ggcatcgttg ttgagaaaac cggcccgtat aacctgctgt
     1921 tcctgttcag catcggtatt gataagacca aagcattgag cctgctgcgt gctctgactg
     1981 acttcaaacg tgcgttcgac ctgaacctgc gtgtgaaaaa catgctgccg tctctatatc
     2041 gtgaagatcc tgaattctat gaagatatgc gtattcagga actggctcag aatatccaca
     2101 aactgattgt tcaccacaat ctgccggatc tgatgtatcg cgcatttgaa gttctgccga
     2161 caatggttat gactccgtat gcggcgttcc agaaagagct gcacggtatg accgaagaag
     2221 tttacctcga cgaaatggta ggtcgtatta acgccaatat gatccttccg tatccgccgg
     2281 gagttcctct ggtaatgccg ggtgaaatga tcaccgaaga aagccgtccg gttctggagt
     2341 tcctgcagat gctgtgtgaa atcggcgctc actatccggg ctttgaaacc gatattcacg
     2401 gtgcataccg tcaggctgat ggccgctata ccgttaaggt attgaaagaa gaaagcaaaa
     2461 aataattagc tcgtacaagg gaagtggctt gccacttccc ttttttgctc ataaggagaa
     2521 cacatgaaaa caccctcaca gccgcgcgcg atatactata tcgtggcgat ccaaatctgg
     2581 gagtacttca gtttttacgg catgcgtgcc ttactcattc tctatctcac ccatcagctt
     2641 ggttttgatg ataaccatgc catcagcctg ttcagcgcat atgcttctct ggtttacgtt
     2701 acccctattc tcggcggctg gcttgccgac cgcctgctcg gcaaccgcac tgcagtgatt
     2761 gccggcgcgc tgttaatgac ccttggccat gtggtgctgg gcattgatac aaattcaacc
     2821 tttagcctgt atctggcgct ggcaatcatt atttgtggct acggtttatt caaatcaaac
     2881 atcagctgtt tgcttggcga gctctacgac gagaacgatc atcgacgtga tggcggtttt
     2941 tcgctgctat atgctgcggg caatatcggt tctatcgccg cgcccatcgc ctgcggcctg
     3001 gctgctcagt ggtatggatg gcatgttggc tttgcccttg cgggaggcgg catgtttatc
     3061 ggtttgttga ttttcttaag tggtcatcgt catttccagt ccacgcgtag tatggataaa
     3121 aaagcgctca ctagcgtcaa atttgcctta ccagtatgga gctggttagt ggtgatgctc
     3181 tgtttagccc cagtattttt taccctgctg ctggagaacg actggtccgg atatttgctg
     3241 gcgatcgttt gcctcattgc agcacaaatc attgcccgca tgatgatcaa attccccgaa
     3301 caccgccgtg ctctttggca aattgtattg ttgatgtttg tcgggacgtt gttctgggta
     3361 ctggcacaac agggcggcag taccatcagc ttgtttatcg atcgctttgt gaatcgtcag
     3421 gcattcaata ttgaagtacc tacagcacta ttccagtcgg tgaatgctat tgcggtgatg
     3481 ctcgctgggg ttgttctggc ctggctggcg tcgccagaaa gccgcggcaa ttcagcattg
     3541 cgcgtctggc tgaagtttgc ctttggccta ctgctgatgg cttgtggctt tatgttgctg
     3601 gcatttgatg cccgacatgc agcggctggc ggtcaagcgt caatgggcgt gatgatatcc
     3661 gggctggcgc taatgggctt tgctgaactc tttattgacc cggtggcgat tgcgcaaatc
     3721 acgcgtctga aaatgtctgg cgtattaacc ggtatttata tgctggcaac aggcgcggtc
     3781 gccaactggc tggcaggcgt cgtggcacag cagacgacag agtcgcaaat tagcggtatg
     3841 gcaattgccg cttaccagcg attcttttct cagatgggag agtggacgtt ggcttgtgtc
     3901 gcgatcatcg tggtattggc ctttgctacc cgttttctgt ttagcacgcc gacgaatatg
     3961 atacaggaga gcaacgatta actgtcgtaa aaacaattgg ttatgaataa attaactttc
     4021 agtttatcac actagcaaat cgagattaat aagtcattta aaccattttt atggttattt
     4081 attagtgata tcaacttgag gtaagcgtta gtttcgataa gataaactga gttattaata
     4141 gtcgaggcag ataatacagt gtaccgatct gtctctttat ctacgctaaa ttgaaagctg
     4201 gatttagagg aaccaaaatg tctgaacgag aaacacgggg agccaatgag gctattgatt
     4261 ttaacgatga actgagaaat cgccgcgaaa aactggcggc actacgtcag caaggtgtgg
     4321 cgtttcccaa tgattttcgc cgcgaccata cctctgacca gttgcacgaa gagtttgatg
     4381 cgaaggataa ccaggaactg gaatccttaa acattgaagt ctcggttgct ggccgaatga
     4441 tgacccgtcg tattatgggg aaagcctcct ttgtaacgtt gcaggatgtc ggtggccgta
     4501 ttcaactgta cgttgcaaga gatagcctgc cagaaggtgt ttataacgat cagtttaaaa
     4561 aatgggatct gggtgacatt atcggtgccc gcggtacgct gtttaagacg caaacgggtg
     4621 agctttccat tcactgtact gagctgcgcc tgctgactaa agcactacgt cctttaccag
     4681 ataaattcca tggtctgcag gatcaggaag tccgttatcg tcaacgttat ctggacctca
     4741 tcgctaacga taaatcccgt caaacgtttg ttgtccgttc aaaaattctg gccgctatcc
     4801 gtcagttcat ggtcgctcgc ggctttatgg aagtagaaac cccgatgatg caggtaattc
     4861 caggtggagc atctgctcgc ccgtttatta cccatcataa cgctctggat ttagatatgt
     4921 acctgcgtat cgcgccggag ctatatctga aacgtctggt tgtaggtggt tttgaacggg
     4981 tattcgaaat caaccgtaac ttccgtaatg aaggtatttc tgttcgtcat aatcctgagt
     5041 tcacgatgat ggaactctac atggcgtatg cggattacca cgatttgatt gaactgacgg
     5101 aatcactgtt ccgcactctg gcacaagagg tactgggtac cactaaagtc acttatggcg
     5161 agcatgtgtt tgatttcggc aaaccgtttg aaaaactcac catgcgcgaa gcaatcaaaa
     5221 aatatcgtcc agaaaccgat atggcggatc tggataattt tgatgctgct aaagcattag
     5281 ctgaatctat cggaattacg gtagagaaaa gctgggggtt gggacgcatt gtcacggaga
     5341 tctttgatga agtggcagaa gcacatctga ttcagccaac ctttatcacg gaatatccgg
     5401 cagaagtgtc accgctggcg cgccgtaatg atgttaaccc ggaaatcacc gaccgttttg
     5461 aattcttcat cggtggtcgt gaaattggta atggttttag cgaattaaac gacgcagaag
     5521 atcaggctga acgtttccag gaacaggtta acgctaaagc tgcaggtgac gacgaagcca
     5581 tgttctatga cgaagattac gtaactgcgc tggaatatgg tctgccgcca accgctggtc
     5641 tgggtattgg tatcgaccga atgattatgc tgtttacaaa cagtcatact attcgcgacg
     5701 ttattctctt cccggcgatg cgcccactga aataaatttc actttaatga acgaagcagt
     5761 caggcgactg cttcgttcgt cattccgcaa atccagtttt gaggagagac gcttcgtttc
     5821 aagataacca gcgggtccac tcaactacag ctcctaaaag agagaaaaaa gtaatgccac
     5881 aggcagactc attggccagg ttattccgac cagaaatgca ctaagtaaac gaatatgtgt
     5941 tttc
//
LOCUS       DAZGAP010000250         5942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGAP010000250 DAZGAP010000000
VERSION     DAZGAP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.251"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..1014
                     /locus_tag="ACHLAM_004758"
     CDS             106..1014
                     /locus_tag="ACHLAM_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO7856166.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1060..3051)
                     /gene="tkt"
                     /locus_tag="ACHLAM_004759"
     CDS             complement(1060..3051)
                     /gene="tkt"
                     /locus_tag="ACHLAM_004759"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7856167.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3329..4087
                     /gene="loiP"
                     /locus_tag="ACHLAM_004760"
                     /gene_synonym="yggG"
     CDS             3329..4087
                     /gene="loiP"
                     /locus_tag="ACHLAM_004760"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO7856168.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4135..5055)
                     /gene="speB"
                     /locus_tag="ACHLAM_004761"
     CDS             complement(4135..5055)
                     /gene="speB"
                     /locus_tag="ACHLAM_004761"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO7856169.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5191..5922)
                     /locus_tag="ACHLAM_004762"
     CDS             complement(5191..5922)
                     /locus_tag="ACHLAM_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856170.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1439 a         1578 c         1550 g         1375 t
ORIGIN      
        1 atgttttttc gctatacccc tttatagatt ctgaattctc acctgtcggc tattttctcc
       61 tcacctcatg tgacatattc cccactttac tcaaggaaga ttcatatgaa agcacctgtt
      121 tcaataacct catttcgccc acaaaaatct attgctatcg tcattggcgt gcttgccgtg
      181 gttgtgctgc catttctctc gtattacacc gttaatgaag gtgaacgcgg cattttgctg
      241 cgctacggaa aaatcgtaaa agttgccgag ccgggtctgg gttttaagat cccttttatg
      301 gaaagcgtgg agaaaatttc cacgcgtaat caggctgtgg tttatcaggg attacaggcc
      361 tacagccgcg accagcaacc ggcacaaatg acagtatcag tcagcttcca cattaaacct
      421 tcagaagcag gcgcggttta taccacctac aacaccattg aagcattaaa agatcgtctg
      481 attgtccgtc agttgccaac tcaactggag aacgttttcg gccaatatac cgccatcagc
      541 gccgtacagg atcgtacaaa actggttcag gatctacaaa atgcaatgcg taaagccgtt
      601 gttggcccgg tggtgattga tggtgtccag attgaaaata ttgatttttc tgacgcttat
      661 gaaaaatcga ttgaagatcg aatgaaagca gaagtcgcca ttgccacacg taagcaaaat
      721 ctggaaacag agaaaatcca ggcgcaaata gcggtaactc aggcccaggc agaagcagac
      781 agtaaactgg cggcagccaa agcagaagct gagactattc gcgtaagagg cgcggcagag
      841 gctgaaacta ttcgtttaaa aagtgcggct gaagcagaag cgatccgcct gcgcggtgaa
      901 gctctgcggg ataatcccgg acttgttgcc ctcaccactg ctgagcgttg ggacggtaag
      961 ctacccgata ccatgatccc cggcagtaca gttccgttta tctctactaa gtaaaaaaag
     1021 ggccgctttc gcggcccttc tcacaaagca tcttaccgat tacagcagcg ctttcgcttt
     1081 cgcaacaacg ttgtcaacgg tgaaaccgaa ctcttcaaac agttgctctg caggagcaga
     1141 ttcaccgaag gtggtcatgc cgacgatagc accgttcagg ccaacatact tgtaccagta
     1201 gtcagcaata cccgcttcca ccgcaacacg agcggtaacc gctttcggca gtacggattc
     1261 acggtaagcc gcatcctgct tgtcgaacgc gtcggtagac ggcatggaaa ccacgcgcgc
     1321 cttcacgcct tcggcagtca gtttttcgta ggcggcaaca gccagttcaa cttccgaacc
     1381 ggtcgcgatg aagatcagtt ccggctgacc ggcacagtct ttcagcacat aaccaccgcg
     1441 cgcgatgttt gccagctgct cttcagtacg ctcctgctgc gccaggttct gacgggagag
     1501 gatcagcgcg gtcgggccgt cctgacgctc aacgccgtat ttccacgcga ccgcggattc
     1561 aacctggtca catggacgcc atgtggacat gttcggggtc acgcgcagag aagcgacctg
     1621 ctctaccggc tggtgagtcg ggccatcttc gcccagaccg atggagtcgt gggtgtaaac
     1681 catcacctga cgctgtttca tcagcgcagc catacgtacg gcgttacgtg cgtattccac
     1741 gaacatcagg aaggtggagg tgtacggcag gaaaccaccg tgcaaggaga taccgttagc
     1801 aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacccg cagcatcttc
     1861 gttgattgct ttagaaccag accacagggt caggttagac ggtgccaggt cagcagagcc
     1921 gccgaggaat tccggcaaca gcgggccgaa agcttcgata gcattctgag acgctttacg
     1981 gctggcgatt ttcgccggat tagcctgcag tttagcgatg aattctttcg ctttggcatc
     2041 gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagcggctt cctgcggata
     2101 agctttggcg taagccgcga atttctcgtt ccatgcggat tctttcgcct ggcctgcttc
     2161 tttcgcatcc cactgagcat agatttcaga cgggatttcg aacggtgcat atttccagcc
     2221 cagctgttcg cgggtcaggg caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg
     2281 cgtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg
     2341 tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg atggatgccg cgtcatgacc
     2401 gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt
     2461 aaaccagcct tcaacgtgac catcgataga aataccgttg tcatcgtaga acgcaatcag
     2521 tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat
     2581 gcagccgtcg cccatgaagg cgtaggtgta gtggtcgacg atgtcgtggc ccggacggtt
     2641 aaactgcgcc gccagcgttt tttctgcaat cgccataccg actgcgttgg caataccctg
     2701 acccagcgga cctgtagtgg tttccacgcc agcggtgtaa cccacttccg gatggcccgg
     2761 agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt
     2821 gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc
     2881 acggtcagcc caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc
     2941 ggcaatgtca gccataccca tcggggcgcc cgggtgaccg gatttggctt tctgtactgc
     3001 gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc
     3061 agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc
     3121 cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg
     3181 ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctgctcgc ccgtccgctg
     3241 cgcttttcct tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat
     3301 aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag
     3361 cgtggcaacg gtactgaccg gttgccagaa tatggactcc aacggactgc tctcatcagg
     3421 agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca
     3481 ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa
     3541 acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta
     3601 caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt
     3661 ctatagcggg ctgatggata tgatgacgga taacgaagtc gaagcggtga tcggtcacga
     3721 aatggggcac gtggcgttag gccatgtgaa gaaagggatg caggtggcac ttggtacaaa
     3781 tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc ggcagtttat ctcaatcaca
     3841 actcggtgat ctgggcgaga aattagttaa ttcgcaattc tcccagcgcc aggaagcaga
     3901 agccgatgat tattcttacg atcttctgcg ccaacgcggc atcagcccgg caggtcttgc
     3961 caccagtttt gaaaaactgg cgaagctgga agaaggccgt caaagctcaa tgtttgacga
     4021 ccatccagca tccgccgaac gcgcccagca tattcgcgat cgtatgagcg cggatggagt
     4081 taagtaagca ttttattacg ccggatttga cgcataacgc atcttatccg gcgcttactc
     4141 gccctttttc gctgcctgaa tatacagcat ctccagcgcc agcgtcgccg cagccagagc
     4201 ggtgatttcc gactgatcgt atgccggagc cacttccact acgtccatcc caacaatgtt
     4261 gagatctttc aggccgcgta ccagtttaat agcgcgatcg gaggtcaggc cgccaattac
     4321 tggcgtaccg gttcccggcg caaatgcagg atccaggcag tcgatatcaa aggtcaggta
     4381 aaccggcata tcacccacaa tctgtttcac ttgggcgata acgtcatcca cgctgcgatc
     4441 gttcacctgg caggcgtcca gcacggtaaa gccgttgtct ttatcgaact cagtacgaat
     4501 accaatctgc acggaatgat tcgggtcgat cagaccttct ttcggcgcgg tatagaacat
     4561 ggtgccgtgg tcaaattcac aaccgttcgc ataggtgtcg gtgtgggcgt caaagtgtac
     4621 cagcgccatt ttgccgaaat gcttcgcatg agcacgcagc agcggcagcg taacaaagtg
     4681 gtcaccaccg aaggagagca tacgcttacc ggcagccagc agcttctcgg cgtgcgcctg
     4741 cagcttctcg ctcatctcac gggcatctcc gaaggcatat accagatcgc cgcagtccac
     4801 gacgttcagg cgctcacgca tgtcgaaatt ccacgggaag cgattgtgtt cccaggccag
     4861 attcgtcgaa acctgacgga tcgctgccgg accgtggcga ccacccgcac gaccagaagt
     4921 ggccatatcg aacggcacgc cagtaatcac ccagtcagca tcgctgtcat atggctggaa
     4981 gttcatcggc aggcgtaaaa aaccaaaggc gttggaaacc agtgagttat cgtattgatg
     5041 acctaaggtg ctcatggatg gactcctatt acaaagtcga tataaaaaaa cccttccgcg
     5101 tcgttaggcc cgacgaggaa gggttggatt tgcgaaacac aattttgtgc taattatcgc
     5161 cttaaatggg cgaggcatca agcgaggttg ctatttccga cacggtggcg gcgcatagcc
     5221 attgttatct gccggaaagg cttgtccaga gcacggcggc agcgcgtttt gcgccgggcc
     5281 ttgtaattca aaggcggcaa agccgattac accggtatta cgcacatcgc cttgcgccga
     5341 attggctgcg taggaatcag caacgttggc gaactgaaaa gccgcttcgg tatgtttatc
     5401 tttgcggaat cctttaatcg ctaacgaatc tccggcattc acaatatagc cgttgttatt
     5461 cagcgaacct tgcttgccgt tcagcacatc caggccatca accgtagcaa cgatctcgta
     5521 gttggtatta cgactgtaat tacggatata taattgataa ttcattccga ctctggcatg
     5581 gacaatatag cgtggttcat tattggatga atcataaacc cgcgtaatag gtagagaatt
     5641 aaaattacca tcgcgaatag catattccag ttcgttaata cgaatgctat aaacgcggtc
     5701 atagccggaa ggaacagtag tcgaataact aataacgatc atctctgccg gatcgcgcca
     5761 gactcgttca gcagatacgc cctgaaccga tgagtgtaca tccccacccc aggcggtgcc
     5821 gacattgcct gatgcactgc tattatactg cgcattactg gaacaaccgg ccagcagcac
     5881 ggctaatgct atcaacgcgc tacgtacttt ccattttttc ataaccgttc cctctgcatt
     5941 ta
//
LOCUS       DAZGAP010000251         5925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGAP010000251 DAZGAP010000000
VERSION     DAZGAP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.252"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..761
                     /gene="mdaB"
                     /locus_tag="ACHLAM_004763"
     CDS             180..761
                     /gene="mdaB"
                     /locus_tag="ACHLAM_004763"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO7856171.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     ESDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            792..1106
                     /gene="ygiN"
                     /locus_tag="ACHLAM_004764"
     CDS             792..1106
                     /gene="ygiN"
                     /locus_tag="ACHLAM_004764"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO7856172.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1151..2038)
                     /locus_tag="ACHLAM_004765"
     CDS             complement(1151..2038)
                     /locus_tag="ACHLAM_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO7856173.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2035..2982)
                     /locus_tag="ACHLAM_004766"
     CDS             complement(2035..2982)
                     /locus_tag="ACHLAM_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7856174.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAGLTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(2993..4030)
                     /locus_tag="ACHLAM_004767"
     CDS             complement(2993..4030)
                     /locus_tag="ACHLAM_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO7856175.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4039..5022)
                     /locus_tag="ACHLAM_004768"
     CDS             complement(4039..5022)
                     /locus_tag="ACHLAM_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO7856176.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLVFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILIFDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5019..5828)
                     /locus_tag="ACHLAM_004769"
     CDS             complement(5019..5828)
                     /locus_tag="ACHLAM_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7856177.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1681 a         1614 c         1362 g         1268 t
ORIGIN      
        1 acttatagga agttgatttc agtattgtca ttttcagaaa atatttatcc cgcatcggtg
       61 aatcagagca agatcagact tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt
      121 tcaccgtacc gctctgtgac gtactatagt cgacaaacgt ctcaccttga ggttaaaaaa
      181 tgagcaacat cctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga
      241 acgacaccct gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgtccgca
      301 tcgttcgcgc cgagagcgac tacgatgtca aagccgaagt acaaaacttt ctctgggctg
      361 atgtggtgat ctggcagatg cctggctggt ggatgggcgc gccgtggaca gtgaaaaaat
      421 acattgatga tgtattcacc gaaggtcacg ggactctgta tgccagcgat ggtcgtaccc
      481 gcaaagatcc gtcgaaaaaa tacggttctg gcggtctggt acaaggtaaa aaatatatgc
      541 tttccctgac ctggaacgca ccaatggaag ctttcaccga aaaagatcag ttcttccacg
      601 gggttggcgt tgacggtgtg tatctgccgt tccataaagc aaaccaattc cttggtatgg
      661 aaccgctgcc gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata
      721 ctgaagaata tcgcaagcat cttgtggaaa tttttggtta actagagctc tggctttaga
      781 aggagttaac catgattaca gtaatcgcag aaatccgtac tcgtcctggt caacatcacc
      841 gtcaggcagt gttggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt
      901 gccacggcta tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac
      961 cggattctat cgtgatgatt gagcagtggg aaagcatcgc gcatcttgaa gcgcatctgc
     1021 aaaccccgca catgaaagca tatagcgaag ccgtaaaagg tgacgtgctg gagatgaata
     1081 ttcgtatcct gcagccgggg ctttgacatt tctaaaaaga ggccggtgct gctaccggtc
     1141 tcagcccaga ttagccattc tttcttcccg ttttcagtcc tcgatggaaa tcgttaattc
     1201 gcttaatggt tttaaccgca cgctgcggca ctgcggaagc taccgaccag acaatcattt
     1261 ccagacacaa caataccgta ccatgtagcg gcgttttccc tttttcatcc ccgcgcggaa
     1321 catgaatcac gatactggcg tctttgctaa aacgtgaatc cagcgcgttt gtcagcaaaa
     1381 taacagggat ccccagccgc ctcgcctcac gaagcattgt cagcccttcc cggtgcgcag
     1441 atttttgcgc catcatgatc agaacatcgc cacgttgaag cgcgatcagt tgctcggcaa
     1501 ggccaatacc cgtacggttt agtgccgtgg caggcaaacc tatgcggttg aatagtcgtg
     1561 cggtatagtc cgccagaata cccgaagcgc caatgccaaa aatagcgact tgccgtgcct
     1621 gcaccaacaa cgctacagct tgcgcaagcg cgtggcggtt ttcaggctct gacaggacat
     1681 tacaggtata caaatgccct tcaagaacaa agtcgattgc ggtgttaaca tcgctcgtca
     1741 agttactcac cgtcgtagac atcttttcgc tggaactaag ggctggacca agccactgtt
     1801 ccagagtgcg tttcagatca cgcagcccgg caaaccccag ggcctgtata gccctgataa
     1861 ctgtggcatc ggaagtgttc aacgtggccg caatttccat cgccgtttgc tcgagtacca
     1921 cttcacgatg ctcattaatg tagctggcga caactcttaa cccaggcgtt aactgactcg
     1981 cgcgagtgcg aaaacgctca ccaaaaatat caacccgcac tttatctttc tggatcattt
     2041 agcctccggt agaggtcgcc ctgcaatttg tgattgtatc tgcgcagcca tcagccccag
     2101 agatgcaaag gagttagaaa ttgcctcttc acgagaaatc agcacataac gcccggaacg
     2161 acaggcagtc agaaactgac accatcccgg catgactttt tccattgctg ccagctcatc
     2221 ctgaggttta ccaccggtgt caccgcgcca ggttgcaaaa acaaaatcgg catccagttc
     2281 aggtaaacgc tccgcgctca catccattcg cccaccttca ggaatgctct caatcagcgg
     2341 cggaaaacga aaacccgcat cgcgtaatac ccgccccagc gaatgatagc tatgcatcac
     2401 attaattttt ccctggtttg cctgaatcac cgataccgtt attttttggc tgtccagcgt
     2461 cgccttcagc gcattgattt gtgcctgata gcggcgttca agtatcgcca gctgcgactg
     2521 cgttcctgta agtcctgcca gcttgcgata gatttctggc gcaccccctt tgagatgatc
     2581 aatgctcacc gtgggcgcaa ttttttcaag ttgttcaatg ggcgtattac gggtcggttc
     2641 tgtgatgatc aggtcaggct ttgccgccac aatcgcttca atatcaatat cagcggtacc
     2701 aataaaggcg atcgatgaat tatcaaagtc gacacctgtc aataacgcgc cagatcgaat
     2761 gaaatggcta ccgtcaggcc gagttcgccc gtggctggcg acaggaggta caccgagctc
     2821 aatcagagga atagtgatat caagatcgtg taaagagaca attctctttg gatgagcagg
     2881 aacagtgact ttgcgcccta aatcatcagt aaatacctga acaggttctg ttgcacggga
     2941 aaaaaatgac aacagtatca gcagagaaaa aaataaacgc atataactcc cgttaaaaac
     3001 tgttcctgcg ctgccagagc agtagcaaaa aaaatggtcc accaatcagt gagataatga
     3061 ttccggcagg cagttgcaaa ggcaaaaaag ccaaacgacc aatgctatcc gccagaataa
     3121 ctaacaacgc ccccgatacc gcgctcccca gcagtaatgt cgtctgcccg ccacgtagaa
     3181 taaaacgtga tatatggggt gctatcaatc cgacaaaacc gatgttacca acacacgaaa
     3241 cgcagacggc tgtcaggatt attggtgcgg cgacacgtaa caacgccaga cgagaactat
     3301 tcacccctaa cccggttgca acctgatgac caagcagagc gatatccgcc gtcctggcgg
     3361 tgaacagcaa taaaagagcg gctggcaaca tccaacaggc tgaaataccg actaacatcc
     3421 agttagctgc atgcaggctt cccgataacc acatcaatgc agtttgtaca tctcgaacat
     3481 ctgcagtggt cataaataca ccgataccgg cggcgaaaaa ccaggaaaca ccaataccaa
     3541 tcagaacaaa tcgcgggcgg gaaatatcgc gcgcacaaaa aatcacgatt aaagccacca
     3601 acaagccacc agcaagacca gccactggac gccagaacat tcccagcatt ggaaactgga
     3661 aaatcagcca cagcaccgcc acgctacatc cttctttgac gccgattaaa ccaggatcgg
     3721 caaggccgtt acgcgcaatg gattgcatcg cggcccccgc catacctaac atggctccgc
     3781 atagcaatgc catgatgact ctcggcaagc gaatatcctg cacaatataa cgtgcgtctg
     3841 tgcttacgtt ttctggagca aacaacgctc gtcctacttc cgatgccggg acagaaaaag
     3901 aaccatgcat cagaccgaat atcagtattc caaccgcaaa cagaaacagc ccaccaagca
     3961 acaccagatt tttagggcgt accagtgtcg aaaacttacc aacacgtagt gggcgtagcc
     4021 cgacgttcat tatccgactc atttgaacat cctcgtcgcc ataatgacaa aaacaggcgc
     4081 accaactaac gccgtcatga caccggtcgc taactcatgg ggcgtgaaaa gcgtccgagc
     4141 aataatatct gccagtaata acaaaagcgc gccgacacaa gctgaaagag ggagcagaac
     4201 gcgcagatcc gctgatacca gacgccggac aatctgcggt actagcaggc cgacaaaacc
     4261 aatggggcca gcaatcgata cggctgcgcc acagagcaac gcgatcgcaa gcagggtgaa
     4321 tgttcgcgtg cggagtaccg agacgcccag cccctgcgcc atgcgatcgc ccagtgccaa
     4381 catgttaaga gagggcgcaa ggtaaatggc taacacaaat ccccccagag aaaaccaggc
     4441 ggacgtaccc agcgtcaccc aatcctgccc cgctaaatca cctgctagcc atgtccgcat
     4501 cgccagcagt gtttgttcgt caaaaatcag gattgcagcc gttattgagg acacgaacgc
     4561 cgatagcgcc acgccgcata aagtaacttt cattggcgtt aaccctgaac gccctgcgga
     4621 ggaaagcagc aatatcaaca ggaacaataa cgcgcccccc gtagatgcca gcagtgggcg
     4681 tccaaccgga aagaccagtc caagcgcact ggcggcaacg acagcaagag cggctccggc
     4741 atttaagcct aaaatatgtg gctcccccaa tgggttacga atgacagcct gcaacaaagc
     4801 accagcaaca ccaagagaag caccggtaag caatgctgcc gctaaacgcg gcaaacggag
     4861 tcttacaatt acgttgtgat cgaagttttg cggatcaaaa tgaagaaatg cctgcaacac
     4921 cgtttgtggt gcaataaatc gggcaccaat ccctggatat accacgcatc ccaccagcag
     4981 taaggcgagg aaaattacag taaccagccc tgtgcgcatc atactttttc tacaccacga
     5041 aacggcatga agaacggctt tcccgtcaga ggatttattg atgcgtgaac atcaacgtca
     5101 aagacagctt ttaccagttc aggcgtgcaa tgctccccct cattcaacac acgcaccact
     5161 ttcccctggc gtaaaaatat caacgtatcg ccataattga cggcaaaatt cagatcatgc
     5221 aacaccacga cgactgtacg cccgtgatga cgcgttaaat catgtaataa ctcaagaatt
     5281 tcaacctgat aacgtaaatc aagccaggtt gtgggttcat ccagcaggat ataaggcgtc
     5341 ttttgcgcca acaccatcgc aatccagcaa cgctggcgct gcccaccaga gagtttttca
     5401 acaggtaaat gtgcaaactc ctgcgtaccg gttaatttca gagcctcttc taccgcctgc
     5461 tcgtcggcgt cgctccattg acgaatgaaa ttctgccagg gaaaacgccc acgcgacacc
     5521 agttcataaa cggttaagcc ctccggcaat aatggcgact gcggcaagat cccaagtcta
     5581 cgggataacg ctttcgttgg ttgctcgtga atcgctttgc catcaagcaa tatcgaaccg
     5641 cccataggtt gcaagatacg tgcaatggtg ctgagcaacg tcgactttcc acagccgttt
     5701 gccccaacca gaacggtcat tttttctgtg ggaacggaga aagaaacacc atcaacaatg
     5761 atcttcttgt gatacccggc agaaacattg tcgaggatca gcccctgccc ttttgcgtta
     5821 tgtgccacgt gtacgccaac ttaatcaaag acaaacaaaa ataaaactca ttctcctttg
     5881 agagtttgac gccacaaaat gtagtagtca agcagttaaa agcca
//
LOCUS       DAZGAP010000252         5908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGAP010000252 DAZGAP010000000
VERSION     DAZGAP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.253"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..97)
                     /locus_tag="ACHLAM_004770"
     CDS             complement(<1..97)
                     /locus_tag="ACHLAM_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFO7856178.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQM"
     gene            complement(173..790)
                     /gene="yncG"
                     /locus_tag="ACHLAM_004771"
     CDS             complement(173..790)
                     /gene="yncG"
                     /locus_tag="ACHLAM_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO7856179.1"
                     /translation="MIKVHGVPGWGTTISEMMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISSIAEAVCQLPKLQEVLKRNEII"
     gene            1057..2556
                     /gene="ansP"
                     /locus_tag="ACHLAM_004772"
     CDS             1057..2556
                     /gene="ansP"
                     /locus_tag="ACHLAM_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO7856180.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2669..3730)
                     /gene="yncE"
                     /locus_tag="ACHLAM_004773"
     CDS             complement(2669..3730)
                     /gene="yncE"
                     /locus_tag="ACHLAM_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO7856181.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3972..>5908
                     /gene="pqqU"
                     /locus_tag="ACHLAM_004774"
     CDS             3972..>5908
                     /gene="pqqU"
                     /locus_tag="ACHLAM_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO7856182.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYK"
BASE COUNT         1423 a         1429 c         1501 g         1555 t
ORIGIN      
        1 acatctgcac aatgtgaata gttagtttct tttcagagga aatgaaataa acaagctgaa
       61 tgaaagggag agtgatataa attaaaaaac ataacatgaa tctaattaat tacgtccatc
      121 attgtggata aagttactga ataataaata acataatctt ctggtatccc attcaaataa
      181 tttcattcct ttttaatact tcttgtaatt tcggtaactg acatacagct tcagcgatcg
      241 agctgatatt ctgcgcatta tcgtgaaacc attcatgccc cggcccccat gtgcgcatgg
      301 tgcaaagata acagtccacc agcgttagtt gctcaccaaa cgcataaggt tcagcggtca
      361 attgtgaatt cagccagata taaagcgatt tacgatattc aataacatta ttctttaact
      421 gttcgggtgc gtcaggagcc caacgctcag ggtaatcggc gaaagtgaat gttggataga
      481 cattagcaac cagccagacc aatagccgtt gaaactgctg acgttcggcg cgcccaaccg
      541 gtggcgcaag gtccgggcga cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca
      601 taatctcgtc attttccagc gccagtgtcg gaatctgaca caacgggttc agggttttca
      661 ataactcgcg tgaagttccc tcgtggtcaa aaccactgac atcaacgaac tgataaggaa
      721 tatcagccag tgttagcatc atctcactga ttgtagtgcc ccatccgggt acgccatgca
      781 ccttaatcat atgcccctca caatctttta aagattaagt ctaggacagc acacaggtta
      841 accagcaaaa gtatttgtca ttttttcgtc agttgtatga atccccttca gaattgtaag
      901 gggatgtttt gcatcgttta tcgataacgg gtaggatgct gcgccgatag ttttataccc
      961 ataaagaata atggtgataa ctatcatcgc caggatgaat aaacattgtt catggcaact
     1021 tatatgactt tttcattaaa gcaatcaggg agagcaatga gtaaacacga caccgacact
     1081 tcagatcagc acgccgcgaa acgccgctgg cttaatgccc acgaagaggg gtatcacaaa
     1141 gcgatgggca atcgccaggt gcagatgatc gccattggcg gcgcgattgg tacgggtttg
     1201 tttttaggcg cgggagcccg actgcaaatg gcggggccag cactggcact ggtctattta
     1261 atctgcggct tgttttcgtt ttttattctg cgtgcattgg gtgagctggt gctacaccgc
     1321 ccttccagtg gcagttttgt ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat
     1381 gttgcaggct ggatgtactt catcaactgg gcgatgacgg ggattgtcga tattaccgcc
     1441 gttgcactgt atatgcatta ctggggcgca tttggtggtg taccgcagtg ggtctttgcg
     1501 ctcgctgcgc tgaccatcgt tggcaccatg aatatgattg gtgtgaaatg gtttgccgag
     1561 atggagttct ggtttgcgct tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt
     1621 acagtgttcc tcggtagtgg tcagccgctg gatggcaaca ccactggctt tcatttaatt
     1681 accgataacg gcggcttctt cccccacggt ttgctacctg cgctggtgtt gattcagggc
     1741 gtagtgtttg cttttgcctc cattgaaatg gtgggtacag ctgccggaga atgtaaagat
     1801 ccgcagacca tggtgcctaa agcgattaac agtgtgattt ggcgtattgg cctgttttac
     1861 gtcggctccg tggtgttgct ggttatgtta ttgccgtgga gcgcgtatca ggcggggcaa
     1921 agtccgttcg tgacgttttt ctctaaactg ggtgtgccgt acatcggcag cattatgaac
     1981 attgtggtgc tgaccgctgc cctctccagc ctgaactcag gtctgtactg caccggacgt
     2041 attctgcgct cgatggcgat gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt
     2101 cagcatgtgc cgtatgccgg gattctggcg acactggttg tgtatgtcgt cggcgtattc
     2161 ctcaactatc tggtgccgtc gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga
     2221 atcatcgctt catgggcgtt tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa
     2281 gaaggcaaag cagcggatgt cagttttaaa ctgcctggcg cgcccttcac ttcctggctg
     2341 acattactgt ttttgctgag cgtccttgtg ctgatggcgt tcgattaccc gaacgggact
     2401 tatactatcg cggcgctgcc gattatcggt attctgctgg ttataggctg gtttggtgtg
     2461 cgcaaacgcg ttgctgaaat tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa
     2521 caggaaattg tgtttaagcc tgaaacggcg agctaattgc aaaattgccc ggctacgtgc
     2581 cgggcaaatt caaattactg acgaaggtgc cggatacggc gtgataaaag tcaggcaagt
     2641 ctctccattt tcaccctcgc catatctatt acagcgcaat acgaatcaca tcgtccggct
     2701 gggtagcttc ctgctgttta gtggattttt gtttcacact gacatacagc gttttgccat
     2761 cggcagacag tgccaggctg ttcggatgag tcggcgtatc gaacgttttc accactttat
     2821 agcttttcgc gtcaatcaca ctgactttac ctgcctgacg atgcgtcacg taggcttcat
     2881 tacgcgcggg gttaaacagc acagccagtg attccggtgc cgcaaccttc gccagaatat
     2941 tgccattacg ggtatcgacc actaacactt ctgccgcttt agagtcggtg ataaatgcac
     3001 gctgccttgc ggtatcaagg ctgatgttga taaagaagtg ctctttgccg tcatccagca
     3061 gctttttacg gctgaggatt ttattgtcgg cggtgtcgat ggtaatcaat tcgccgtcag
     3121 cgttagtggt gtaaagacgt ttgcctttgc tatccagcgc taaaccggta ctcattttac
     3181 cggtgttctg gatggcggtt ttcagtttga tattctcgcc atcaacgacc caaatcacgc
     3241 tctctttacc aataccactg atgtaaacgg tgttcgtggc atcgtctgcc accagctcac
     3301 gcggttgcag cgggcgcacc tcttcagtgc gcttacgatc atccagcacc agacggcctt
     3361 tcacttcgcc cgttttggca tctatcgccg tgaccgcgct gtttacagtg ttaccaaacc
     3421 acaacgtctg agtcgtgtta ttgatggtag caccaaacgg cttgagatcg ttatggatcg
     3481 cctgcgtcac ttccagagta accggatcaa gacgataaac cacgccgcct ttatccagtt
     3541 tgcggctttg cgaagtggcg agccacagcg cgttttcttg ctggctataa gccatttcgt
     3601 aggcaccttt acctaccgct ttacgcagca tttcttctgc ggcctgcgta ctgaatgatg
     3661 aagcaacaag caatgaacct aacagtaatg aaccacgcag gcgcgatgaa aacagatgac
     3721 gtaaatgcat gacgactccc tttgatgaac cgatattttt gctgcttcac aatgcttcgc
     3781 taagcgtaat catcgttacg ctcttgttat aaatgggaat ggtaatcatt attttctcaa
     3841 atgtggagca tttcttgccg ttacgactac aattttctca attacttaac atatcgtgcc
     3901 gtttcgtttt ccataaattt acaattttat taccatgacg gtgttttccc attcttaacc
     3961 tggctttttt catgaagatt ttttccgtcc gacagaccgt ttttcccgcg ctgcttgtcc
     4021 tttcccccac cgtttttgcc gctgatgaac agaccatgat tgtcagtgcc gcgccgcagg
     4081 tgatttcaga actggatacc ccagcagccg tgagcgtggt ggatggcgag gagatgcgcc
     4141 tggcaacacc gcgcattaac ttgtctgaat cactgactgg cgtgcctggt ttgcaggtac
     4201 aaaaccggca gaactatgcg caagatttac agctgtcgat tcgcggattt ggctcccgct
     4261 ccacttacgg aattcgcggt attcgcctgt atgttgacgg tattcccgct accatgcccg
     4321 acggacaagg gcagacatcc aatatcgatt taagcagtgt gcaaaatgtg gaagtgctgc
     4381 gtggcccctt ctctgctctg tatggcaacg cgtctggcgg tgtaatgaat gtcatcaccc
     4441 agaccggaca acagccacca accattgaag ccagtagtta ctacggcagt tttggcagct
     4501 ggcgctatgg gctgaaagca acgggcgcaa cgggagacgg cacacagcca ggcgatgtcg
     4561 attacaccgt ctcaaccacg cgctttacga cccacggcta tcgtgatcat agtggcgcac
     4621 agaaaaattt agccaatgcc aaactgggcg ttcgcattga tgacgccagc aaattaagcc
     4681 tgattttcaa tagtgtggat atcaaagcag atgacccagg tgggctaacc gaagcagaat
     4741 ggaaggcgaa tccacaacaa gcgccccgtg cagaacagta cgacacgcga aaaaccatca
     4801 agcaaactca ggctgggttg cgctatgaac gtagcctgag cgcgcaagat gatatgagtg
     4861 taatgatgta tgccggagag cgagaaacga cccagtacca gtcaatcccc atgtccccgc
     4921 aacttaaccc gtcacatgcg ggcggcgtga ttaccctgca acgccattac caggggatag
     4981 acagccgctg gacacaccgt ggtgaattgg gcgttccagt cacgttcaca accggcctga
     5041 actacgaaaa catgagtgaa aaccgcaagg gttacaataa cttccgcctg aatcgcggcg
     5101 tgccggaata cgggcaaaaa ggtgagttgc gccgcgacga acgcaatttg atgtggaacg
     5161 tcgatcccta tttacaaacc cagtggcaac tgaccgacaa actttcgctg gatgctggcg
     5221 tgcgctacag ctctgtgtgg tttgattcca atgatcatta cgttactccg ggtaacggcg
     5281 acgacagcgg tgatgccagt tatcacaaat ggctaccagc cggatcgtta aaatatgcaa
     5341 tgaccgatgc ctggaatgtc tatctggcag ccgggcgcgg ttttgaaacg ccgacgatta
     5401 atgagctgtc ttatcgcgct gatgggcaaa gcggtatgaa ctttggttta aaaccctcca
     5461 ccaacgatac aattgagatc ggcagtaaaa cgcgtattgg tgatgggctg cttagtctcg
     5521 cattgttcca gaccgacacc gatgatgaaa ttgttgtcga tagcagtagc ggtgggcgta
     5581 cgacttacaa aaatgccgga aagacccgtc gtcaaggcgc tgaactggca tgggatcaac
     5641 gtttcgcggg agattttcgc gtaaaagcgt cctggacctg gcttgatgcg acctatcgca
     5701 gcaatgtttg caatgaacag gattgtaacg gtaaccggat gccagggatc gcccgtaata
     5761 tgggctttgc gtcgataggt tatgtaccgg aagatggctg gtatgcaggt acggaagcgc
     5821 gttatatggg cgatattatg gcagatgatg aaaatacggc caaagcgccg tcttatactc
     5881 tcgtcggctt attcaccggg tataaata
//
LOCUS       DAZGAP010000253         5756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGAP010000253 DAZGAP010000000
VERSION     DAZGAP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.254"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            265..1428
                     /locus_tag="ACHLAM_004775"
     CDS             265..1428
                     /locus_tag="ACHLAM_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO7856183.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNIFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ENELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1433..3580
                     /locus_tag="ACHLAM_004776"
     CDS             1433..3580
                     /locus_tag="ACHLAM_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO7856184.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3640..4887
                     /locus_tag="ACHLAM_004777"
     CDS             3640..4887
                     /locus_tag="ACHLAM_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO7856185.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5173..>5756
                     /locus_tag="ACHLAM_004778"
     CDS             5173..>5756
                     /locus_tag="ACHLAM_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856186.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLVEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEDE
                     QKPVQPEGGQEAGKTDVSSTGSIEPESGNLSGRVT"
BASE COUNT         1445 a         1227 c         1654 g         1430 t
ORIGIN      
        1 attgaagtat ctcacatttt tattatcttc tgtcagcgcg gaagataatg gcggtagctt
       61 ggatcaattc cggtgtctct gcatttcaat tttactcaat attttacccg gttgcatcat
      121 gatgcctgat tatatttccc gcgtgtcccg gaggttcggg tgaagagaat attatctttc
      181 tgttttcttt tctctgtcca caacgtgttc gcttctccac tgccggccct cctgagggat
      241 gctctcattc atgatccatc agtgatggag gcaagggcag aagccacctc tgcccgttcc
      301 cgtgtggagc aggctcgttc agcacactgg ccggtcgtca cagcaacagg cagtaaactc
      361 ctttcccaga gtcaccgcta cgactatgac tacgactctg aagatatttt gcccggtatc
      421 cgcggtgaaa tgaatatatt cgcttcggga gctattgagg cggatgtcag ccggagtgag
      481 tcggaagccg agtattaccg ttataaagtt gatgaggctg gcgaggaaac aattcgctcc
      541 tttgtttcat tatatcttga tgcgctaagg gaaaagcaat ccattgcggt acttgaacag
      601 agtctctccc ggcataacgc aattcttaat gatcttaaca ccatcagtat tcacgatacc
      661 gggcgggaat ctgaatttgt tcaggccgaa gcccgcaggc tgatggtccg gcagcagata
      721 aatacccgca cccgagtgct caaaaccacg ctgggaaaac tttcgacctg gaccacaaat
      781 ccggtaaccg aagccgatct tgaaaatcct ttttcccgga tgacagagtc cagattgtta
      841 actgatttta cgcagacgcc tcagaaaggg aatccgtcgt ggcttgccag tcaggctgat
      901 gtagagagta aaaaagcggc gctgaaagcg caggagcttg cccggtatcc ccgggtggat
      961 ttaacgggat cagtgacccg ggatgacagg cagataggtg tcagtctgtc ctgggacctg
     1021 ttcaaccgta atgccagtta tggtgtcagt gaaaaggccg cgcagatagc tgcagctacc
     1081 ggacgactgg actctgttgc ccgaatgatt gatgaaaccg gacgattatc gctgataacg
     1141 gtcagacaaa gtcggatgga aatggaaacg ctcagacgtc aggagcaggc ctctgccaga
     1201 gttgtggact tttatcgtct tcagttccgg gtggcaagaa aaacactgat tgaattactg
     1261 aatgcagaaa acgaactgta cagcgtcggg ctttcccggg ttcagacaga ggatcagatg
     1321 ctccacggaa tgctggatta tctgtattcc cagggaatgc tcctgaaatg gagcggagtg
     1381 aatctttctg gtgcagaaga aaaagtaaca gggcagaagg acagataaat aagtgaagag
     1441 tatttttgaa ggattatcac aggtaacggc attactgggt acacctgtat ctgctgagac
     1501 gctggctgcc ggtacagtcc ggacagatgt ctccgggatt gattttcgtt ctgttggcga
     1561 gtttcttcgc agtgaagggt ttgataatca tctttcccgg cgggctccgg aagatattcc
     1621 ttctcttgcg gttcctgtgc ttcttttact gaatgcacag gaagccattg tggtggtgcg
     1681 tattgagggt gccggcctgg cccggcagta tcatgtactc cagcccgggg gactccgtaa
     1741 gacactttct cacgatgaat taatggctgt ttattcaggt tactgctggt ttgttcagcc
     1801 acaggcaaca caggatcgcc gttcggaact gccggagtat gacatgaccg gggcatggtt
     1861 ctggcgggta ctgtggcggt tcaggccata ttacggacag gtcattcttg cgagttttat
     1921 cattaacttt cttgcgctgg ttagctcctt atatgtaatg aacgtttatg accgggtcat
     1981 acccaatcgc tcctgggaaa cgttatggat gctgagtatc ggggtgattg cagccattct
     2041 gtttgagtgt gctgcaaagt taatccggag ctatctgacg gatattgcgg gtaagaaggc
     2101 agacctgatt atcagctcag cactgttaag gcgggtgatg gcagtgaaaa tgtcggacag
     2161 gccggcgtct tccggttcat acgccagcaa tttgcgggaa tatgagtctg tcagggagtt
     2221 tatgaccagc gccacgctgc tggcttttgt ggatttgccg tttatcctgc ttttcatcgg
     2281 tgtcatcggg cttgtggggg gctggcttgc tgttgttcct cttacactgg ttccgctggt
     2341 gattctggca ggaatattac tgcagcgacc gctttcgcgt catattaatg cctccatgcg
     2401 ggaatcatcg cagcgccaag gattacttgt ggaggctctg gacggaatag aaaccctgaa
     2461 gctgaataat gcagttaact gggtccagcg ccgctgggat ggatatacag ccagtgtcgc
     2521 ccgctcgtcc atacgggtaa aaaataccag taatgtcata atctgctttt ccacagcagt
     2581 tcagcaactg aatactgttc tgctggtcat tctggggacg taccttattc acagtgatga
     2641 cccacataac cgaatcacca tgggggcact gattgccgca gttattcttt ccggtcgtgc
     2701 gctggcaccg cttggtcaga tagcatcact ggccacccgt tttcagcagg cccgcctggc
     2761 catgcagggg gtgaatgaca ttgtccgccg tccggtggag cgggaaagtc agacacatta
     2821 tctgacagtg aaggatatcc agggagagtt tcgtttcagt gatgtctcct tttcttataa
     2881 gcccggatta cccgacgcat tgtgcggtct gtcgctctgt atccggcccg gagagcgggt
     2941 ggggattatt ggctcaatag gttgtggtaa atcgacgctg ctgaaaatga tggtggggct
     3001 gtaccgtcag aacaggggac aaattactct tgacggggtt gcgatgcagc aactggaccc
     3061 ctgttttgtc cgggataaag tgtttcttct ggaacagaat ccccggcttt ttctgggaac
     3121 tctgcgtgaa aatctgcagc tttcgcgtct gccgggaagc ctgtcggatg tggatatgat
     3181 taatgcagcc ggacgtttcg ggtttgaccg ttttatccgg catcatcccc gcggacttga
     3241 tatgcagaca ggggaaggcg gtcagggact gtcaggagga cagaggcaga tggttgccct
     3301 gacgagaatg gtactgggtt ctccccgggt ggtactgctt gatgagccca cgacaggtct
     3361 ggacctgcag actgaatctg ttgttctgaa aggtctgagc cactggagcc ggggacgcac
     3421 aatggtggtg gtgacacatc gtctgcaggt tctgaatatt gttgaccgta ttgtcgtgat
     3481 gaatgaaggg cgggtaatga tggacggtcc ccgtgatgaa atactgaaaa aactgtcagg
     3541 gaaacagcag agtggggaga gcagagcgat aacttcatga gcgtcaaccg gataagaaaa
     3601 aagagcagat atgtttaaca gtttgtggac cgcagggata tgacagaaaa aacggaacgg
     3661 aataaagcgt cgtcagaggt ggtcaggcag tcagacctgg cgctgttgac agatgtacag
     3721 gcagcccttc agcaggaaaa gcatcatgcc cttttcctga tggtgatttt tctgctggta
     3781 ctggtagtga cgtttgttat ctgggcatgg aacagtcctc tggatgaagt gacccgaggt
     3841 caggggagta ttattcccgg cagcagggag caggttatac agacccttga cccgggtata
     3901 ctgaaatcac ttgaggtcag ggaaggcgat attgttgaga aaggtcaggt actgctgaca
     3961 ctggatgata cccggagctc tgccatgctg agggaaagtg aagcccgggt gaataacctg
     4021 gaggccgtca gggcaagact ccgtgcagaa gcttattccg aatcactgac atttccggat
     4081 aacgttcctg aggatttgcg ggagcgggaa agtacggtct attttctccg gaagacggag
     4141 ctggcgcaga gcattgccgg tctgaagcag agtaaggcgc tgctggacag ggaaatagca
     4201 atgacgcggc caattgtgcg tgaaggggca atgtcggagg tcgaactgtt acgtatgcag
     4261 cgtcagtcag cggagctgca gttacagatg gatgagaaac agaataaata cctgacggaa
     4321 gccggtgctg aactggtgaa gacagaagca gagcttgctc aggcaaaaga gaatatggct
     4381 ggcagagccg acccggttga gcgttcacgt ctcagggccc ccctgagagg tatcgtaaaa
     4441 aatatacggg taaacacact gggaggggtg gtttctgcag gacaggacat aatggaaatt
     4501 attccgctgg aagaccagtt gctgattgaa gcttatataa atccacggga tgttgcctat
     4561 gtcagaacag ggatgccggc acttgtaaaa ctgacggctt atgactatgc catttatggt
     4621 ggtcttgacg gcgtggtgac actggtaagt ccggatactt tacgtgacca gaaacggccg
     4681 ggagatttaa aactggaccc gaatgaagct tattaccggg tactggttac caccagtaat
     4741 aattatctga cagacaggaa cggaaaaaga ttgccagtta ttcctggcat gatagccagc
     4801 gtggatataa aaaccgggcg taaaacggta tttcagtatc tgattaaacc gataaccaga
     4861 atgaagcagg cacttcagga aagataataa aaaggtcttc cggggaaagt attttaaatc
     4921 aattttaaac gggcagaatg atttcacttt attctgcccg ggtcattgcg catgcctgta
     4981 tgtgatttgg ggctgaagat aatataaatg ttatatctgt caggggaaaa taaatctgaa
     5041 aaaagcggaa tgattaagga ggtccggtca gagaaataaa ttatctgtaa tctgattcgt
     5101 ttttatttaa tgactccctt tagcggataa aacataagag catactggtt ttattaacct
     5161 gaggatactt atatgaaaat gattttcact ggcaaggtca gtggagaaaa aacagtactg
     5221 acagccggag cccgtcatac tgtaaaggca caggccgggg aacagtatgg actggttgat
     5281 gaggtgacag gtctggtccc ggatggtgtg gaagcggacc gttctggtga tgatttaatt
     5341 ctgcgtaaaa aggaagatga tacagaaatc cggattgagg gattctggga agaatgccag
     5401 ccgggtgaaa cacagtgtac agcagtattt aatgttgtgg gtgaaaatgg tcaggtaact
     5461 gaagctgtcc tgacccagga tggccctgta ctggagaatt tcactgcagg gcaaagtggc
     5521 acactggttg aaggtatcaa taacgggatt atctgggggg ggattgcttt tggtgcaggg
     5581 ctggcggcta ttgcactggc tgccggaggc ggtggtggaa gcagtcgtca taagcgtgca
     5641 actgaagatg agcagaagcc ggttcagcct gaaggcggtc aggaggctgg aaaaacagat
     5701 gtatcatcca caggctcaat tgagccggaa agtggaaacc tctctggacg cgtgac
//
LOCUS       DAZGAP010000254         5720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGAP010000254 DAZGAP010000000
VERSION     DAZGAP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.255"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..129
                     /locus_tag="ACHLAM_004779"
                     /pseudo
     CDS             1..129
                     /locus_tag="ACHLAM_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(328..1452)
                     /gene="yhhJ"
                     /locus_tag="ACHLAM_004780"
     CDS             complement(328..1452)
                     /gene="yhhJ"
                     /locus_tag="ACHLAM_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7856187.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1452..4187)
                     /gene="rbbA"
                     /locus_tag="ACHLAM_004781"
     CDS             complement(1452..4187)
                     /gene="rbbA"
                     /locus_tag="ACHLAM_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO7856188.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYHPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     ACLQEAAGQSNEAEAPPVIHDTTHAARQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMVPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4184..5251)
                     /gene="yhiI"
                     /locus_tag="ACHLAM_004782"
     CDS             complement(4184..5251)
                     /gene="yhiI"
                     /locus_tag="ACHLAM_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7856189.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5314..5427)
                     /gene="yhiY"
                     /locus_tag="ACHLAM_004783"
     CDS             complement(5314..5427)
                     /gene="yhiY"
                     /locus_tag="ACHLAM_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO7856190.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
BASE COUNT         1330 a         1642 c         1486 g         1262 t
ORIGIN      
        1 gagtgcttta ttaactggat gcgtgactgc cattcttcag atgataaaga cgtcattgta
       61 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      121 gtcatttctc gcgtcagtcc ttgcggggag cgggctttcg taatcttgcc ctgaataccg
      181 gactgagtat aaaaaagccg gttaactgaa actgtccagg ttttggtggt cagttcataa
      241 acgccttatc cggcctaaaa aaaactaaaa ttcaataaat tgcaccaatg cgtagaccct
      301 ataagcttac gcatcgggca actgtaatta cgccattgtc ccaattgtct tcctgaatct
      361 cagcagtgcg atggtgaaaa acgcgccgcc aattgccatc agtgtcagga actgcggcca
      421 gacgatttcg aatccggcac cacggtagag aatggcctgc gcgaggctaa caaagtgtgt
      481 cgtcggcatg gtcagcataa tgtcctgcac catctgcggc atactttcgc gcggcgtgga
      541 accaccggaa agcatttgca gcggcagcag caccagaatc accagcagcc ccagttgcgg
      601 cattgaacgc gctatcgtcc ccataaaaat accgattgac gtggtggcaa acagactgag
      661 cgccacgccc agcataaaca gcgggatcga gccttctatc ggtacgccca gcacgccttt
      721 taccatcagc accagcgata atcccgacac caccagcacc accagcccca tcgaccagac
      781 ctttgccatc atgatctcaa acggcgttat cggcatcacc agtaagtgtt ccaccgtgcc
      841 gtgttcacgc tcgcggatca gcgccgatcc ggtcaataca atcgccagca tggtaatgtt
      901 gttgatgatc gccatcaccc cgccaaacca cgcgggatca aggttcgggt taaagcgcat
      961 ccgtgtttcc agcgatacca acggttcgct gttatcacgg tagcgcgcga caaagctgtt
     1021 cacttcaccg ttgataatat tctggatata cccattgccg gtaaatgcct ggctcatgcg
     1081 cgtggcatcg acgttcacct gaatatccgg ctggcgtccg gcgaggacat cacgctgaaa
     1141 attaggcgga atatttatcg cgaaggtata acgtccggcg tccagcccgg catccatctc
     1201 atcggcggtg atcatctccg gtggcaaaaa ccacgggcga tagaagctgt taacgatccg
     1261 gttcgataac tgcgattgat ccatatcggc aatggcgatc ggcgcgaggt tcaacgatcc
     1321 tggcgtaacg gtcgctgacg aatacaccga caccgtaaac gagaagacaa tcagcgtcag
     1381 catcgcttta tcaccgagca gactgcgcaa ctctttgata cccagattaa aaatattacg
     1441 taaatggcgc atcatccctc ctgttttttc agcagcagga tgcttaagcc catcaccagc
     1501 ggtatggcta tcagcaacgg gataaaaagt tgccacaaat cagtcagatc cagcgctttc
     1561 gagaacgttc cgcgggcgat agtcagaaaa tgactggtcg ggtaaacctc gccgatccaa
     1621 cgtcctggac cttccagcga agctaccgga tcgatcatcc cggaaaactg tgtcgccggg
     1681 atcaacgtga taattgccgt accaaaaatg gcggcaatct ggcttttcat aaaggtggag
     1741 atcagcagcc ccattccggt ggcgatgatg atatacagca gcgccgccag ggtgagagtc
     1801 aggaaactgc ctttatgcgg tacgccaaat acaaataccg acagggcgca gagcaggaaa
     1861 aagttcagca tccccagcgc gatgtatggc agctgtttgc caagcaaaaa ttcgctacgg
     1921 gtggtggggg tcacgtaaag gttgataatc gaccctagct ctttttcccg caccacgcta
     1981 agggcgctta gcattgacgg gaccatcatc agcagaagcg ggatcaccgc cggaacaatc
     2041 gccggcaggc tttttacgtc cgggttatag cgatagcgcg tctcaatatt catcagcccg
     2101 ctttggctgg cgggtgtgga ctgtcggctc gccacatcct gtagccagct ctggtgcatg
     2161 gcctgcacgt agccttttac cgtttcggcg cggctcggca tcgcaccgtc gatccagaca
     2221 ccgagttcca cgggcgtacc acgcgcgata tcgcgcccga aattaggagg gatctcaatt
     2281 gccaccgtga tatcgcccgc acgcatccga cgatcaagct cgtcataact ggtgagcggc
     2341 ggctgttcga taaagtaacg ggaaccggag agattgagcg tccacgcctg gctactgacg
     2401 gtttgatcgc ggtcaagcac cgcaaagcgc aggttttcca catccatact gatgccgtaa
     2461 cccattatca gcatcaggat caccgttccc atcagcgcca gcgtcgaacg taccggatcg
     2521 cgtcgcagtt ccagcgcttc gcggcggctg tagctaaaca gacggcgcag gctaaatccc
     2581 tgacgcgccg cgtgggtggt gtcgtgtatc acgggcggcg cttcggcttc gttgctctgc
     2641 cctgccgctt cctgcaaaca ggcgataaat gcctcttcca gacttgcggc tccgcgtttc
     2701 tcaaccagtt cctgcggtgt cccgctggcg agcacttttc cggcgtgcat cagtgagata
     2761 cggtcgcaac gttccgcttc gttcataaag tgggtggaga tgaagatagt cactttgtcc
     2821 tggcgcgaga gatcgaccat caactgccag aacatatccc tcgccaccgg atcgacgcca
     2881 gaagtaggct catcgaggat taacatctcc gggcgatgaa tcaccgccac cgccagcgaa
     2941 agccgctggc gaatgccgag cggcaatgac tccggcagaa catcttcaac gtcgttgagc
     3001 ttaaaacgct cgctcatttc agccactcgc gcgggaattt ccgcttccgg gatgtgaaac
     3061 aaacgggcat gtaactcaag gttttgccgc acggtgagtt cgttatagag cgaaaacgcc
     3121 tgcgacatat agcctacccg acggcgggta tcgatatctt ttggatcaac cggttgccca
     3181 aacagccacg cctcaccttc gctggcgggc agcagtccgg tgagcatttt catggtggtg
     3241 gatttaccgc agccgttcga accgaggaat ccaaaaatct ccccgcgtgg aatgcggaaa
     3301 ttgacgtgat caacggcaac gaaggaacca aaacgcatgg tcagatcgcg cgcttcgatg
     3361 gcaatctctg cgttttcagg gtgatacggt gggatcacta ccgcctgatg cgcctggcgt
     3421 tgcgcttgcg gtaacagatt tataaatgct tcttccagcg tggcgctttg cgtttgctgc
     3481 cgcagctctt cggcgctgcc ggttgccagc acttctccgg cattcatcgc taccagccag
     3541 tcgaagcgtt cggcctcttc catgtaggcg gtggcgacca gcacgctcat attgctctgc
     3601 cgctggcgaa tgctgtcgat caaatcccag aactgggcgc gggagagcgg gtcaaccccc
     3661 gttgttggct catcaagaat caacagttcc gggtcgtgga ttaacgcaca gcacagccca
     3721 agtttttgct tcatcccgcc ggagagtttc cctgccggac gatcacgaaa cggtgctaac
     3781 ccggtgctgg tcagcagctc attgattcgc acttcccgct ccgctttgtc gtgaccgaac
     3841 aggcgagcga aaaaatcgac gttttcatac accgacaagg tgtggtagag gtttttgccc
     3901 agcccctgcg gcatccaggc gatgcgcggg caaacgtcgc ggcgatgctt cgggtcgcgc
     3961 atatcgccgc ccagcaccat cacgctgccc tgctcaatga cgcgggcacc ggaaatcaac
     4021 gacaacaagc tcgatttacc gacaccatcc ggtccaatca gcccgaccat gcaacgggcc
     4081 ggaatatcga gtgtgatatt gttcagcgca acggtttttc cataatgctg gctcacgccc
     4141 gccagttgcg cgacaggcgg gacgggaacc agttccagat gcgtcattgc ggcaacctca
     4201 ccaccaggtc gtcaggccac ggaagttctt cattcacccg cacccacgct acgcccggca
     4261 atccggtttt gacatattcc agatgctgct ggagtaattc cggtgggata cgcgctttga
     4321 cacggaacat caatttcagc cgctcatcgc tggtttcgac ggtttttggc gtgaactggg
     4381 cgacgctggc gacaaaactg atggtcgcgg gaatacgcag atctggtgca gcatcaagaa
     4441 tcaaacgtgc ttcaccgccc agtttcagcg tgcccgcctg ttcggttggc agaaagaagg
     4501 tcatatagac gtcgctgaga tcgaccatat tcagcacccg accgcctgcc gccagcactt
     4561 cgcctggctc ggcaacccga tactgcacgc gtccgtcacg cggggctttc agttcgctgt
     4621 catcgatatc tgcggcaatg cgccgttcag tggcttgtgc cgcttcgacg cgagtttgcg
     4681 cctgaatgat attggtgcgt gccgcctcta tagcggcttt agaagcagaa acctgagctt
     4741 ttgccgattc cagcgcagct cgggcgctct cagcggcggc gcggtcgtca tccaactgtt
     4801 gcgcagaaat tgccccccgt tgtgccagtg agcgggaacg cgtatgacgt tttgctacgg
     4861 agtccagttc tgcctggcgt tgattaacca gcgactgtgc ggcacgagtt tcgctttggc
     4921 gttgctccag caaagcctgc gcggcggcaa cggcactttg tgcttctttg atttgcgcga
     4981 tggcttccag tcgctgttcc tgcaacacgc gagtatccat cttcgccagc acttcacctt
     5041 ctcgaacaaa ctggccttct ttcaccagaa tggtgtcgat acgcccggca attttgctgg
     5101 cgatatccac ttccgtcgct tcaatgcgcc cattgctgac agcaaagcct tccggcacac
     5161 ccgccgggcg caacagccac cacgccacga cggcagccac cgccagtgcc ccgacaaccc
     5221 accacgccag atggcgctta ctcttatcca taatcgatcc gccataatcc ctgtaagcaa
     5281 tcgacgcgtg ttacaactgc caccgaacga tgttcagcgg attcttccat ccgggataag
     5341 aaggaaatgg caaatacgac cagcgcacag agcattgagc gccaatgggg aaggcttagt
     5401 gaaaacgggt agtaatgttg tcatcatcag cattcctggc cgtaaatgaa acacgcatac
     5461 tcataagtga aatcaattaa atactaacag ttatattcat gttgtctatt tgattgccat
     5521 caacttgtga tacatcgcga tgttcttccg ttcgctggtg ttttttccaa aaatattctg
     5581 tacaggttca cagattctgt tgcacaggtt tccgtaacag ggaaacctga tgactatcac
     5641 atagcgttat tatcgcctga tgtgcgaata aataccgaat ataaaataat ttattctgaa
     5701 aagatgatag atgataaagg
//
LOCUS       DAZGAP010000255         5714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGAP010000255 DAZGAP010000000
VERSION     DAZGAP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.256"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..1418
                     /gene="adeQ"
                     /locus_tag="ACHLAM_004784"
                     /gene_synonym="yicO"
     CDS             84..1418
                     /gene="adeQ"
                     /locus_tag="ACHLAM_004784"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO7856191.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1471..1923
                     /gene="yicN"
                     /locus_tag="ACHLAM_004785"
     CDS             1471..1923
                     /gene="yicN"
                     /locus_tag="ACHLAM_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO7856192.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2134..3324
                     /gene="nepI"
                     /locus_tag="ACHLAM_004786"
     CDS             2134..3324
                     /gene="nepI"
                     /locus_tag="ACHLAM_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO7856193.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MTGMIAIFMSFAGQFAFFTYIRPVYMNLVGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3365..3658)
                     /gene="yicS"
                     /locus_tag="ACHLAM_004787"
     CDS             complement(3365..3658)
                     /gene="yicS"
                     /locus_tag="ACHLAM_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO7856194.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3880..4698
                     /gene="nlpA"
                     /locus_tag="ACHLAM_004788"
     CDS             3880..4698
                     /gene="nlpA"
                     /locus_tag="ACHLAM_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO7856195.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4713..5627)
                     /gene="yicL"
                     /locus_tag="ACHLAM_004789"
                     /pseudo
     CDS             complement(4713..5627)
                     /gene="yicL"
                     /locus_tag="ACHLAM_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
BASE COUNT         1350 a         1298 c         1481 g         1585 t
ORIGIN      
        1 gaaatgtgat aattatcact gaaccgttgt tgtcatttgt tttacaaaag caagggatta
       61 aatctcctcc aatggacaaa aaaatgaata atgacaatac cgattacgtg agtaatgaat
      121 cagggacgct ttcgcgatta tttaaactac ctcagcatgg gaccaccgtc cgcacagaat
      181 tgattgcggg gatgaccact tttttaacca tggtatacat cgtttttgtg aatccgcaaa
      241 tcctcggcgc ggcacaaatg gacccgaaag tggtgtttgt taccacctgt ttgattgccg
      301 gtatcggcag tattgcgatg gggatatttg ctaacttacc cgtggcgctg gctccggcaa
      361 tggggctgaa cgccttcttt gcgttcgtgg tcgtgggggc gatgggcatc tcctggcaga
      421 ccgggatggg cgcgatattc tggggcgcag ttgggctatt tctgctcacg ctgtttcgta
      481 tccggtactg gatgatctcc aacattccat taagtttacg tattggtatc accagcggaa
      541 tcggattatt tatcgcctta atgggattaa aaaatactgg cgttattgtc gccaataaag
      601 acaccctggt gatgattggc gatttaagtt ctcacggcgt gttgttaggt attttagggt
      661 tttttattat aaccgtgttg tcatcacgtc attttcatgc cgcggtgctt gtttctattg
      721 tggtgacgtc ttgctgtgga ttatttttcg gtgatgttca ttttagcggc gtctattcca
      781 ttccgcctga tattagcagc gtcattggtg aagtggattt gagcggcgcg ttatcacttg
      841 aactcgccgg tatcattttc tcctttatgc tgatcaacct atttgattca tcaggaacat
      901 taattggtgt aactgataaa gctggcttaa tagatagtaa cggtaaattc cctaatatga
      961 ataaggcgct gtatgttgat agcgtcagtt cggtggcggg tgcgtttatc ggcacctcgt
     1021 ctgttaccgc ctatattgaa agtacttctg gtgtggcagt cggtggtcgc acggggctga
     1081 ctgcggttgt ggtcggcgtt atgttcctgt tggttatgtt cttctcaccg ctggtggcga
     1141 tggttcctcc ttacgcaacc gccggagcgt taatctttgt tggcgtgttg atgacctcaa
     1201 gtctggcgcg cgttaactgg gatgatttta ccgaatcggt acctgcgttt attaccacgg
     1261 tgatgatgcc ctttactttc tcgatcaccg aagggattgc actcggcttt atgtcgtact
     1321 gcatcatgaa agtgtgcacc gggcgctggc gcgatctgaa cctgtgtgtg gtggtggtcg
     1381 cagctctgtt tgcactgaag attattctgg tggattagta actggcgagg gtttccagat
     1441 atcatgagtt ctgattacgc aggagaactc atgatctgga taatgctcgc cacgctggcg
     1501 gtagtgtttg tggttggttt tcgggtgctg acatccgggg ccagaaaagc gattcgccgt
     1561 ctcagcgatc ggctgaacat cgatgtcgta cccgtggagt cgatggtcga tcaaatggga
     1621 aagtcagccg gtgacgaatt tttacgttat ttgcatcgtc cggatgagtc gcacctgcaa
     1681 aacgccgcgc aagtgttgct catctggcaa attgtcattg tcgatggtag cgaacagaac
     1741 ttgctgcaat ggcatcggat tttacaaaaa gcccgccttg ccgctcccat taccgatgct
     1801 caggtcaggc tggcgctggg ttttctgcgc gaaaccgaac ctgaaatgca ggatattaat
     1861 gcttttcaga tgcgctataa cgcgttcttt cagcctgccg agggcgttca ctggctgcat
     1921 taaaaccgct tcgtcttatc agcctacaga ccgcgcctga cctgtagggt ggataaggcg
     1981 ttcacgccgc atccggtaat ctttgccctg ttccgtttcg atcttaactc cagctcaagc
     2041 aacaccgtaa aagttgcaaa aacgttaaat acgtcacaca tttcaatgtt aaagtggtcg
     2101 gctttccctc tgaaacatgc cacgggtaac accatgagtg aatttattgc tgaaaaccgc
     2161 ggcgcggatg ccatcacccg accgaactgg tcagccgttt tctcggtggc gttttgcgtc
     2221 gcctgtctga ttatcgttga gtttttgcct gtcagtttgt tgacgccaat ggcccaggat
     2281 ttaggcattt cggaaggggt tgccgggcaa tcggtgaccg tgaccgcctt tgtggcgatg
     2341 tttgccagtt tgtttattac ccagaccatt caggccactg accgccgcta cgttgtcatt
     2401 ctgttcgccg ttttgctgac gctttcctgt ttgctggttt cctttgccaa ctcattcagt
     2461 ctgctgttaa tcggtcgcgc ctgtctgggg ctggcgctgg gcgggttctg ggcgatgtcg
     2521 gcgtcgctga ccatgcgtct ggtaccgccg cgtacggtgc cgaaggcgct gtcggtgatc
     2581 ttcggcgcgg tttctattgc gctggtgatt gccgcgccgt tgggcagttt tttaggcgag
     2641 cttatcggtt ggcgcaatgt ctttaatgcg gcggcggcga tgggtgtgct gtgtattttc
     2701 tggattatca aatcactgcc ttcgctgcca ggtgaacctt cgcatcagaa acaaaatact
     2761 ttccgcttat tacaacgtcc gggagtgatg accgggatga tcgccatctt tatgtccttt
     2821 gccggacagt ttgctttttt cacttatatt cgcccagtgt atatgaatct ggtgggattc
     2881 ggtgttgatg gcttaaccct ggtgctgctg agttttggta tcgccagctt tgtcgggaca
     2941 tcgctttcgt cgtttattct caaacgttcg gtgaaactgg ccttagcggg cgcaccgttt
     3001 gtgctcgccc tgagcgcgct ggtactgacg ctgtggggaa gcgataaaat tgttgctact
     3061 ggtgtggcga ttatctgggg gctgaccttt gctttaattc ccgttggttg gtcaacgtgg
     3121 atcactcgct cattggccga tcaggcagaa aaggctggat ctattcaggt ggccgttatt
     3181 cagcttgcca atacctgcgg tgcggcaatc ggcggttatg cgttggataa cattggtctg
     3241 acttcgccgc tgatgttgtc cggcacattg atgttgctga ctgcattgtt ggtgactgca
     3301 aaggtgaaaa tgaagaaatc ctgatatcag gtaaagaagc gttggtcagt ggttgttgcc
     3361 gttcttacat atccgggcat tctaccttac ccaacgcttc ccaataattg ctctgattat
     3421 tacgttccat cgccacgatg gcctcgcgta tatgttgttt attgttttca tctgacaaca
     3481 ttaacttttc ccacgcttca tctgataatg acgcctgtgg cgtgcaaatt ttacgtaaat
     3541 cttgcaatac cgtggttttc tcttttgccg actgtagaga actgaatggc gactcggcct
     3601 gagaaatggc tggaaaaatc aggaatacgg taatcatgag tagcatcgtt ggcttcatag
     3661 cggcaacctt ctgcaaatgt gcatcgaatg aagcataagt gtagtttgct tttgccgctc
     3721 tcatcatgct gtacgaaagg tgtttgcgtg gtcagtaaga agtgccagac tttatattcc
     3781 acttttattc ctttttattc ttatcgatag cgtttcgttt tttaaactgc agcgacctta
     3841 ccgctatagt caggtaatca ttaataaaag gataaaaaaa tgaaactgac aacacatcat
     3901 ctacgggcgg gggccgcatt attactggca ggaattctgc tggcaggttg cgaccagagc
     3961 agcagcgatg aaaagcacat taaagtgggc gttattaatg gcgcagaaca agatgtcgcg
     4021 gaagtcgcta aaaaggtggc aaaagagaaa tatggcctcg atgttgaact ggtaggattt
     4081 agcggttcgt tgctaccgaa cgatgcgaca aatcatggcg agcttgatgc gaacgtcttc
     4141 cagcatcgac cctttcttga acaagataat caggcgcatg gttataaact ggtagcggtg
     4201 ggaaatacgt ttgtcttccc catggcgggt tattccaaaa aaatcaaaac cgtggcgcaa
     4261 ataaaagaag gcgcgacagt ggcgatcccc aacgacccta ccaaccttgg tcgcgcgctt
     4321 ttactgttgc aaaaagagaa gctaattact ctgaaagagg gaaaagggtt actacctacc
     4381 gcgctggata taaccgataa tccacgccat ttgcagataa tggagctgga aggggcacag
     4441 ttgccacgtg tactggacga cccgaaagtg gatgtagcga ttatcagcac cacttacatc
     4501 cagcaaacag gactttctcc ggttaacgac agcgtattta ttgaagataa aaattcgccg
     4561 tatgtgaata ttctggtggc acgggaagat aataagaatg cagaaaacgt gaaggaattt
     4621 ctgcaatctt atcaatcacc cgaagtcgct aaagcggcag aaaccatctt taacggaggc
     4681 gcggtgcctg gctggtaatt ctcactcatg ccgcgcgccg gacgatttat ttttctggcg
     4741 cggcggcggg aatccattga aatcaaaatc accgatgaca gaatcagcag cgttcccagc
     4801 cagtccggca atgtgaaggt gatccccagc aacagcaaag agagcagcgc gctacttaat
     4861 ggttctgcac agcttaaaat gctggctttt ggaccgccaa ttaattgtgc tcctttcagg
     4921 tacagactaa atgtcaggga cgtaccaatg accaccaaat aaaaaaacgc cagaatcaaa
     4981 ctgccgttaa cgacaaagtt tgtcccttgt ctggcataaa agggcagcag gatcagaccg
     5041 ccaatcagca tactccagcc gacgaccggt aacgtgccat agcgggcaat cagcgttgag
     5101 ggataggtgg tatagaatgc agcagcaaat gccgaggcaa tgccccagaa taacgcggca
     5161 ggagagatcg ataatgacgt cggattaccg tgtgtcacca ataaaaaagt cccgatcagc
     5221 gatgtcaaaa tagcgcagaa aaccagaatg cccgggcggg atttacgcac cagtgagaac
     5281 caggcgacga taatagtcgg tgagaggaat tgcagcaccg ttgccgtggc tgcgttcgat
     5341 ttttctatgg ttagcaaaaa agtgagctgt acggttagcg cgccaaccac ggaaaaaatc
     5401 agtaggctaa tggcatcttt gtgattgtta ataatagaaa agattttatc accatgaaca
     5461 aatgatagcg tcagcagaat caaaccggcg aatatcaaac gcgtcatagt caaaaactgc
     5521 gacgacatct ggctttgctc catgatgtat tgcgcgcaga cccctgaact tccccacaac
     5581 acggcggcaa tcagaacgtt cagcatcccc tttctggtgg aacccatttt tccctctgca
     5641 tactgttttg tcttttcatc aatatacgtt aaggatacca ctaccgatga cggtgaggga
     5701 gtagagtaat caat
//
LOCUS       DAZGAP010000256         5669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGAP010000256 DAZGAP010000000
VERSION     DAZGAP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.257"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..180)
                     /locus_tag="ACHLAM_004790"
     CDS             complement(<1..180)
                     /locus_tag="ACHLAM_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO7856196.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMM"
     gene            complement(284..1021)
                     /gene="bamD"
                     /locus_tag="ACHLAM_004791"
     CDS             complement(284..1021)
                     /gene="bamD"
                     /locus_tag="ACHLAM_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO7856197.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            1156..2136
                     /gene="rluD"
                     /locus_tag="ACHLAM_004792"
     CDS             1156..2136
                     /gene="rluD"
                     /locus_tag="ACHLAM_004792"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO7856198.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            2133..2864
                     /gene="yfiH"
                     /locus_tag="ACHLAM_004793"
     CDS             2133..2864
                     /gene="yfiH"
                     /locus_tag="ACHLAM_004793"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO7856199.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2994..5567
                     /gene="clpB"
                     /locus_tag="ACHLAM_004794"
     CDS             2994..5567
                     /gene="clpB"
                     /locus_tag="ACHLAM_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO7856200.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1408 a         1353 c         1544 g         1364 t
ORIGIN      
        1 catcatttcg aggggaagac ggcgcttgcg tagcgttaca gtacctgatt cggcaaggca
       61 acgagagatg agttcggggt cgaggtaatc ccccagagaa gtcagtgggt tacgcagaga
      121 atcgtaacgg gataccagat caagagcctg tccaatgtgc ataaaaaaat ccggaaacaa
      181 gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct taactgatcg
      241 gcattacagc tcaagggctg ccgttttgtg tttcaggttt ctgttatgta ttgctgctgt
      301 ttgcggcgat gattttcgct actttttcag cttgcgcatt caactgcatc tgacggtatg
      361 cattttccat cagcggcagc gcatcacgcg tagcctgggt atccgggtag tcgcgcaaca
      421 tgccttctac gcggttaacg acggcaaccc atgcaccacg ttctgtatag tactcggcaa
      481 cggagtattc atatttcgcc agacgatctt tcaggaatac cagacgtttg gtggcatcgg
      541 tggtgtactg actgttcgga tagccgcgca ccagtttgga aaagtcacta aacgcagctc
      601 gtgcatgttg aggatcgcga tcgctacggt caacgccaaa gaacccttgc agcgcactgt
      661 catccagcgc catattggtc aggccacgca tgtacatgac ataatcgata ttcggatggg
      721 tcgggttaag gcgaataaaa cgatcgatgg cagcctgtgc taacggcaaa tcggcgtttt
      781 tatagtaggc gtagatgaga tccagctgca cctgctgcga atacggacca aacggatagc
      841 gattatctaa cgcttccagt tgcgttattg cctgtctcca gttaccgtcc tgcagctttt
      901 gttgtgcagt cgcgtaaatt tcatttggcg gattatcagg tacttcttcc tttgaccccg
      961 agcaacccgc caaaaacagg cttagtgtgg cggctgccac cagatatttc atgcgcgtca
     1021 tgacgttttg actttcctca aaatgtaata cgggagattc tctgttcctg ctcccggtta
     1081 agaccagcta caatagcaca ctatattaaa cggcaaagcc gtaaaacccc aacgataaac
     1141 gaagaagcag tatatatggc acaacgagta cagctcactg caacggtgtc cgaaaaccaa
     1201 ctcggtcaac gcttagatca ggctttggcc gaaatgttcc cggattattc acgttcgcga
     1261 ataaaagaat ggatcctcga ccagcgcgtg ctggttaacg gcaaagtttg tgataagccg
     1321 aaagaaaaag tattgggtgg cgagcaggtg gccatcaacg ctgagattga agaagaagcg
     1381 cgttttgaac cgcaggatat cccgctggat atcgtctatg aagatgaaga tatcatcgtc
     1441 attaataaac cacgcgacct ggtggtacat cctggcgcgg gtaacccgga tggtacggta
     1501 ctgaatgcgt tgcttcatta ttatccgccc attgccgatg taccgcgtgc gggcatcgtc
     1561 catcgtctgg ataaagacac caccggcctg atggttgtgg caaaaaccgt tccggctcag
     1621 acgcgtttag tcgaatcttt gcaacggcgt gaaattactc gtgagtatga agcggtggca
     1681 attggtcata tgaccgctgg cggtacagta gatgaaccaa tcagccgcca cccgaccaaa
     1741 cgtactcaca tggcggtgca tccgatgggt aaaccggcgg tcactcacta tcgcatcatg
     1801 gaacacttcc gtgtgcacac gcgtctgcgg ttgcgtctgg aaaccggacg tacgcaccag
     1861 atccgcgtgc atatggccca tatcactcat ccgctggtgg gcgatccggt ttatggtggc
     1921 cgtccgcgtc cgccaaaagg tgcttcggaa gcgtttatct ccacgctgcg taagtttgac
     1981 cgccaggcgc ttcatgcaac catgctgcgt ctttatcacc cgatctccgg tatcgaaatg
     2041 gaatggcatg cgcctattcc agaagatatg gtggagctga ttgaggtgat gcgcgccgat
     2101 ttcgaagagc ataaggatga agtggactgg ttatgagtaa gctgattgtc ccgcagtggc
     2161 cgctgccaaa aggtgttgcg gcctgtagct ccactcgtat cggcggcgtg agcttgcccc
     2221 cgtatgattc gctcaacctc ggtgctcatt gtggcgataa cccggatcac gttgaagaga
     2281 atcgtaagcg actttttgct gcgggcaatt tgccttcaaa accggtctgg cttgagcagg
     2341 tacacggcaa agatgtgctt aagcttactg gcgaacctta tgcctcaaaa cgagcagatg
     2401 cctcttatag caacacgccg ggcacggttt gcgcagtgat gactgccgac tgcctccccg
     2461 tgctgttttg caatcgagcg ggaacggaag tcgccgccgc tcatgctggc tggcgtggac
     2521 tgtgctcagg cgtgctggaa gagacggttt cctgttttgc tgataatccg gaaaatattc
     2581 tcgcctggtt agggccggca attggtccac gcgcgtttga agtgggggcg gaggttcgcg
     2641 aggcgtttat ggcagtagac gccaaagcga gtacagcttt cattcagcat ggtgataagt
     2701 atctggcgga tatttatctc cttgcccggc agcgtctggc gaacgtcggt gtcgaacaaa
     2761 ttttcggcgg tgaccgttgt acatatacgg aaaatgagac tttcttctct tatcgtcgcg
     2821 acaagaccac cggtcgtatg gcaagtttca tttggctgat ataacctaaa gaatcaagac
     2881 gatccggtac gcgtgatttt cttttcacat taatctggtc aataaccttg aataattgag
     2941 ggatgacctc atttaatctc cagtagcaac tttgatccgt tatgggagga gttatgcgtc
     3001 tggatcgtct tactaataaa ttccagcttg ctcttgccga tgcccaatca cttgcactcg
     3061 ggcacgacaa ccaattcatc gaaccacttc atttaatgag cgccctgctg aatcaggaag
     3121 ggggttcggt tagtccttta ttaacatccg ctggcattaa tgctggccag ttgcgcacag
     3181 atatcaatca ggcattaaat cgtttaccac aggttgaagg tactggtggt gatgtccagc
     3241 catcacagga tctggtgcgc gttcttaatc tttgcgacaa gctggcgcaa aaacgtggtg
     3301 ataactttat ctcgtcagaa ctgttcgttc tggcggcact tgagtctcgc ggcacgctgg
     3361 ccgacatcct gaaagcagca ggggcgacca ccgccaacat tactcaagcg attgaacaaa
     3421 tgcgtggagg tgaaagcgtg aacgatcaag gtgctgaaga ccaacgtcag gctttgaaaa
     3481 aatataccat cgaccttacc gaacgagccg aacagggcaa actcgatccg gtgattggtc
     3541 gtgatgaaga aattcgccgt accattcagg tgctgcaacg tcgtactaaa aataacccgg
     3601 tactgattgg tgaacccggt gtcggtaaaa ctgccatcgt tgaaggtctg gcgcagcgta
     3661 ttatcaacgg cgaagtgccg gaagggttga aaggccgccg cgtactggcg ctggatatgg
     3721 gcgcgctggt ggctggggcg aaatatcgcg gtgagtttga agaacgttta aaaggcgtgc
     3781 ttaacgatct tgccaaacag gaaggcaacg tcatcctgtt tatcgacgaa ttacatacca
     3841 tggtcggtgc gggtaaagcc gatggcgcaa tggacgccgg aaacatgctg aaaccggcgc
     3901 tggcgcgtgg tgaattgcac tgcgtaggtg ccacgacgct tgacgaatat cgccagtaca
     3961 tcgaaaaaga tgctgcgctg gagcgtcgtt tccagaaagt gtttgttgcc gagccttctg
     4021 ttgaagacac cattgctatt ctgcgtggct taaaagaacg ttacgaactg caccaccatg
     4081 tgcaaattac tgacccggca attgttgcag cggcgacgtt gtctcatcgc tacattgctg
     4141 accgtcagct gccagataaa gccatcgacc tgatcgatga ggcagcatcc agcattcgta
     4201 tgcagattga ctcaaaacca gaagaactcg accgactcga tcgccgtatc attcagctca
     4261 aactggaaca acaggcgtta atgaaagagt ctgatgaagc cagtaaaaaa cgcctggata
     4321 tgctcaacga agaactgagc gacaaagaac gtcagtactc cgagttagaa gaagagtgga
     4381 aagcagagaa agcatcgctt tctggtacac agaccattaa agcggaactg gaacaggcga
     4441 aaatcgctat tgaacaggct cgccgtgtgg gggacctggc gcggatgtct gaactgcaat
     4501 acggcaaaat cccggaactg gaaaagcaac tggaagccgc aacgcagctc gaaggcaaaa
     4561 ctatgcgtct gttgcgtaat aaagtgaccg atgccgaaat tgctgaagtg ctggcgcgtt
     4621 ggacggggat tccggtttct cgcatgatgg aaagcgagcg cgaaaaactg ctgcgtatgg
     4681 agcaagaact gcaccatcgc gttattggtc agaacgaagc ggttgatgcg gtatctaacg
     4741 ctattcgtcg tagccgtgcg gggctggcgg atccaaatcg cccgattggt tcattcctgt
     4801 tcctcggccc aaccggtgtg gggaaaactg agctttgtaa ggcgctagcg aactttatgt
     4861 ttgatagcga cgaggcgatg gtccgtatcg atatgtccga gtttatggag aaacactcgg
     4921 tgtctcgtct ggttggtgcg cctccgggat atgtcggtta tgaagaaggt ggctacctga
     4981 ccgaagctgt gcgtcgtcgt ccgtattccg tcatcctgct ggatgaagtg gaaaaagcgc
     5041 atccggatgt cttcaacatt ctgttgcagg tactggatga tgggcgtctg actgacgggc
     5101 aggggagaac ggtcgacttc cgtaatacgg ttgtcattat gacctctaac ctcggttccg
     5161 atttgattca ggaacgcttc ggtgaactgg attatgcgca catgaaagag ctggtgctcg
     5221 gtgtggtaag ccataacttc cgtccggaat tcattaaccg tatcgatgaa gtggtggtct
     5281 tccatccgct gggtgaacag cacattgcgt cgattgcgca gattcagttg aaacgtctgt
     5341 acaaacgtct ggaagaacgt ggttatgaaa tccacatttc tgacgaggcg ctgaaactgc
     5401 tgagcgagaa cggttacgat ccggtctatg gtgcacgtcc gctgaaacgt gctatccagc
     5461 agcagatcga aaacccgctg gcacagcaaa tactgtctgg tgaattggtt ccgggtaaag
     5521 ttattcgcct ggaagttaat gaagaccgga ttgtcgccgt ccagtaatga taaaacgagc
     5581 ccttcggggc tcgtttttgt ctataaatta gacggaaaag actatgttta agatgttttg
     5641 cctgaaaaat gagcgaacga taaagtttt
//
LOCUS       DAZGAP010000257         5601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGAP010000257 DAZGAP010000000
VERSION     DAZGAP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.258"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..1625
                     /locus_tag="ACHLAM_004795"
                     /pseudo
     CDS             74..1625
                     /locus_tag="ACHLAM_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
     gene            1651..2103
                     /locus_tag="ACHLAM_004796"
     CDS             1651..2103
                     /locus_tag="ACHLAM_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO7856201.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTFIPERLHKT"
     gene            2256..3530
                     /locus_tag="ACHLAM_004797"
     CDS             2256..3530
                     /locus_tag="ACHLAM_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7856202.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3523..5601
                     /gene="mchF"
                     /locus_tag="ACHLAM_004798"
                     /pseudo
     CDS             3523..5601
                     /gene="mchF"
                     /locus_tag="ACHLAM_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
BASE COUNT         1595 a         1068 c         1381 g         1557 t
ORIGIN      
        1 gaagctgtca ggacacttac ttgctctttt atgaacactc ttttatgaaa attgaaacgg
       61 agaatactgc cagatgagtc atcagtgttc actttctgaa ctgaatgaaa acctggtgcc
      121 tttcactgcc aggcagatca aggcctcatt aatctggtgt gcagaggatg tcagaaatcc
      181 agacgagctg caaaatgcct gcagttatat tatcgatcct ggcagtacag cttctgccaa
      241 agtgttccat gcagagcgct atggtggcag tggtattcag cgtaatggag gtggtgcacg
      301 ttgtgggttt gatggtaact accaggttaa aggaatagga agtaatccgt tggttggcga
      361 aggtactgac gaacgtcatt ctaatggtgc actcggcgct gttcatgcaa tattatgagg
      421 ctttgtgggg agaagtactg gctcaaatat taccttatag tgctgtgcgg gttcgggcgg
      481 ttttacttac agatctctat actgaaaagg catttgagcg ctccggtagg aaatcacgaa
      541 gagccctgtt ggtacgtgag cctgttgttc gtccggcgca ttttgaacgg gcaccttact
      601 tccaagtaaa accggagtat tccagtctgc taattcacga tgcctgtcgg gttagatctg
      661 tgatccacaa gctgccagga tatctacctg taccaccgga agaaattgat gctgaagcac
      721 gaactgatcc ccggatttat tgcattgagg ggttatgtga actggcacgt cgtgaggcct
      781 ggcaaatggc attttgtcga acacgtttcc tgagattgac aacttctcct tctaatattg
      841 caatggatgg cagattaatg gattttaacg gactcagttg tctgtttccg ggagattccc
      901 cagctgattt tgggtataaa ctaagattag ctgaactggc aaaagaaccg atggtactta
      961 tgcaagggct gtctgatctc tgcttgtata tcggaaaata tatgtttgac cctgacttca
     1021 ctcttgcagc ccgtttgaag gttgaggaga tatttcagaa aactttccat gaagcatgtt
     1081 attactgtta tctagaactg ttgggtattc ctggagaatt tataacacaa aaagagatac
     1141 ctgatatatt gaaagaactg gttaacagtt ttgttgcatt actcaatcaa tactgcgaga
     1201 aatcacatgc ccaagatatt gtcaatcagg atggttcacc attgcaaaag ttggttgtga
     1261 cgctaatcca tcataggcat aatcaaaagc aggcactgaa tagtagcatc aagaatgatg
     1321 tttatttcac cgttgcacaa cagtgttttt cccaagccat ccactggctg acgcaaggca
     1381 gtaccagacg tcagataaat gcctcattac tcctgaaaga aattgaacat cataccatga
     1441 aaaggctgca acccagggaa gagctgagga aagaaaatat gtgcgaaaaa attgccatcc
     1501 tgctggataa tcatggcgat gatccccttt ttttacaaga agcaatttct gatatgaaaa
     1561 attttatgct taagttttcc agagatgcat ttggatatct tgaaccgata agaaacacag
     1621 tgtaaaaaat atataaggga aatagtaatc atgtcttata taagggaaac catcagagga
     1681 aaagatgaat ggactgttta tgaacagatc ggttttgcgg tcagttgtat gctctacaat
     1741 cgtaattaca gtctgtatcc ggtgttaacc attcaatact ggactgaata tgcgatacag
     1801 cataatcaga ttaaattcct gtttgattca cgaggttttc cactggcgta tattacctgg
     1861 gcatatcttg aggctgatac agaagcgcgc ctgctcaggg atccagaatt caggttgcat
     1921 ccgtctgaat ggaatgaaga tggaaggatt tggattctgg atttctgttg taaaccaggc
     1981 tttggtcgaa aagttattga ctatctcata cagcttcagc catggggaga aggagaagta
     2041 cgatggttaa gcaggcgaaa gaaaattgtg acattcatcc ctgagcggct gcataaaacg
     2101 tagtacctct gaagatacag agataattgt aaattacggg gtaaatgcat cgctgatact
     2161 attttgacag gactctgtat tttctggctt tgattatagc atttcgctta accagtgaag
     2221 gtattacagg aaaagtgtga gtaagaggag ctattttgtt tcgtcaggat gctttagaaa
     2281 acagaaaaat gaagtggcag ggacgggcaa tattacttcc cggaatacca ctatggttaa
     2341 tcatgctggg aagcatagtg tttattacgg catttctgat gttcattatt gttggtacct
     2401 atagccgccg tgttaatgtc agtggtgagg tcacaacctg gccaagagct gtcaatatat
     2461 attcaggtgt acagggattt gttgtcaggc agtttgttca tgaaggacag ttgataaaaa
     2521 aaggggaccc tgtttatctg attgacatca gtaaaagtac acgtagtggt attgtcactg
     2581 ataatcatcg tcgggatata gaaaaccagc tggttcgtgt ggacaacatt atttcccgtc
     2641 tggaagaaag taaaaaaata acgctagata ccctggaaaa acaacgcctg caatacacag
     2701 atgcgtttcg tcgctcatca gatattatac agcgtgcaga ggaagggata aaaataatga
     2761 aaaacaatat ggagaattac agaaactatc aggcaaaagg gctgattaat aaagatcagt
     2821 taactaacca ggtggcatta tattatcagc aacaaaacaa tcttctcagc ctgagcggac
     2881 agaacgaaca gaatgctctg cagataacca ctctggagag tcagattcag actcaggctg
     2941 cagattttga taaccgtatc taccagatgg aactgcaacg gtacgagtta cagaaagaac
     3001 tggttaacac tgatgtggag ggcgaaatca ttatccgggc gttgactgac gggaaagttg
     3061 actccctgag tgtcactgtc gggcaaatgg tcaataccgg agacagcctt ctgcaggtta
     3121 ttcctgagaa cattgaaaac tattatctta ttctctgggt cccgaatgat gctgttcctt
     3181 atatttcggc tggtgacaaa gtgaatattc gttatgaagc ctttccggca gaaaaatttg
     3241 ggcagttctc tgctacggtt aaaactatat ccaggactcc tgcgtcaaca caggaaatgt
     3301 tgacctataa gggagcacca cagaatacgc cgggcgactc tgttccctgg tataaagtca
     3361 ttgcgatgcc tgaaaagcag attatcagat atgacgaaaa atacctccct ctggaaaatg
     3421 gaatgaaagc cgaaagtaca ctatttctgg aaaaaaggcg tatttaccag tggatgcttt
     3481 ctcctttcta tgacatgaaa cacagtgcaa caggaccgct caatgactaa cgggaatttc
     3541 agacaaatta taaatcagct tgatatgcgc tggcgacgtc gtgttccggt tattcatcag
     3601 acggagaccg ctgaatgtgg actggcctgc ctggcaatga tatgcggtca ttttggtaag
     3661 aatattgacc tgatatctct tcgccggaag tttaatctct cggcccgtgg agcaaacctt
     3721 gcaggaatca atggaatagc ggagcagctg gggatggtca cccgggctct ttcactggag
     3781 ctggatgaac ttggtgccct caaaatgccg tgtattctcc actgggattt cagtcacttc
     3841 gtcgtgctgg tcagcgtaaa gcgtaaccgt tatgtactgc atgatccggc cagaggcaga
     3901 agatatctcg gtcgggagga aatgagccgg tattttacgg gcattgcact tgaggtctgg
     3961 cccggaagtg aattcctggc ggaaacccag cagacccgca taagtctccg ttcactgatt
     4021 aacagtattt acggtattaa aagaacactg gcgaaaattt tctgtctgtc agttgtaatt
     4081 gaagcaatca atctggtaat gccggtggga actcagctgg ttatggatca tgcgattccg
     4141 gcgggggaca gagggctgct gacgcttatt tctgctggcc tgatgttctt tatattgctc
     4201 agggccgcgg tgagtatgct gcgtgcatgg tcctcactgg ttatgagcac gctcatcaat
     4261 atacagtggc agtcgggtct gtttaaccat cttctcagac tgccgctggc gttttttgaa
     4321 cgccgtaaat taggtgatat ccagtcgcgt tttggctccc ttgacacttt gagggccacc
     4381 tttaccacct gtgttgttgg ggcaatcatg gacagtatta tggttgtggg tgtttttgtg
     4441 atgatgctgt tatatggagg atatcttacc tggatagtgc tcggttttac catggtttac
     4501 gttcttattc gtctggtgac atacggctat taccggcaaa tatcggaaga aactcttgtc
     4561 agggggcccg ggccagctcc tattttatgg aaagcctgta tggtattgcc acggtaaaaa
     4621 tccagggtat ggctgggatt cggggaacac actggcttaa cctgaaaata gatgcgatca
     4681 attcaggtat taagttaacc aggatggatt tgctcttcgg ggggataaat acttttgttg
     4741 ccgcctgtga tcaggtggcg attttatggc tgggtgcaag ccttgtgatc gataatcaga
     4801 tgacaatagg aatgtttgtg gcatttggtt cttttcgtgg gcagttttcg gatcgggttg
     4861 cttcgctgac cagttttctt cttcaactga gaataatgag tctgcataat gagctcattg
     4921 cagatattgc actacatgaa aaggaagaaa agaaaccgga aattgaaatc gttgctgaca
     4981 tgagcccggt ttcactggaa accactgatt taagctaccg gtatgacagc cagtcagcac
     5041 aggtattcag tggtctgaat ttgtctgtgg ctccgggaga aagtgtggct ataactggtg
     5101 cctccggtgc cggaaaaacc acattaatga aagtattatg tggactgttt gaaccagata
     5161 gtggaaaagt actggttaat ggcacggata tacgtcaact tggaataaat aattatcacc
     5221 gtatgatagc ctgtgttatg caggacgacc gactattttc aggatcaatt cgtgaaaata
     5281 tctgtgggtt tgcagaagaa acagacgacg aatggatgac agaatgtgcc aaagcaagtc
     5341 atattcatga tgtgataatg aaaatgccaa tggggtatga aacgttaata ggtgaactgg
     5401 gggaaggtct ttccggcggt caaaaacagc gtatattcat tgcccgagct ttataccgga
     5461 aacctggaat attatttatg gatgaggcta caagttctct tgatacagaa agtgaacgtt
     5521 tcgtgaatgc tgctataaaa aagatgaata tcacccgggt gattattgca catagagaga
     5581 caactttaag aacagttgat c
//
LOCUS       DAZGAP010000258         5548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGAP010000258 DAZGAP010000000
VERSION     DAZGAP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.259"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..189
                     /gene="rrf"
                     /locus_tag="ACHLAM_004799"
     rRNA            74..189
                     /gene="rrf"
                     /locus_tag="ACHLAM_004799"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            204..279
                     /locus_tag="ACHLAM_004800"
     tRNA            204..279
                     /locus_tag="ACHLAM_004800"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Thr,seq:ggt)
     gene            319..434
                     /gene="rrf"
                     /locus_tag="ACHLAM_004801"
     rRNA            319..434
                     /gene="rrf"
                     /locus_tag="ACHLAM_004801"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(497..1255)
                     /gene="yhdZ"
                     /locus_tag="ACHLAM_004802"
     CDS             complement(497..1255)
                     /gene="yhdZ"
                     /locus_tag="ACHLAM_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO7856203.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1263..2366)
                     /gene="yhdY"
                     /locus_tag="ACHLAM_004803"
     CDS             complement(1263..2366)
                     /gene="yhdY"
                     /locus_tag="ACHLAM_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7856204.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKNQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2376..3557)
                     /gene="yhdX"
                     /locus_tag="ACHLAM_004804"
                     /pseudo
     CDS             complement(2376..3557)
                     /gene="yhdX"
                     /locus_tag="ACHLAM_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3625..4650)
                     /locus_tag="ACHLAM_004805"
     CDS             complement(3625..4650)
                     /locus_tag="ACHLAM_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7856205.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5081..5302)
                     /locus_tag="ACHLAM_004806"
     CDS             complement(5081..5302)
                     /locus_tag="ACHLAM_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856206.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1539 a         1403 c         1318 g         1288 t
ORIGIN      
        1 gagagaagat tttcagcctg atacagatta aatcgacagg tcataacgag acgtgttgat
       61 aaaacagaat ttgcctggcg gccttagcgc ggtggtccca cctgacccca tgccgaactc
      121 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
      181 ctgccaggca tcaaatttag cgtgctgata tggctcagtt ggtagagcgc acccttggta
      241 agggtgaggt ccccagttcg actctgggta tcagcaccac tttttaggtt aaagttcggc
      301 agattagaaa agaatttgtc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
      361 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
      421 gggaactgcc agacatcaaa tcaagcgaaa ggccatccga aaggatggcc tttttgcttt
      481 tcgaactaac attcaattaa tggatcacct gcgataaaaa tgccctcgta cgctctgatt
      541 taggatgcgc aaaaaactca tccggtgcag cttgttccac tatctcccca cgatccataa
      601 aaatcaccct gtcagcgacg gttcgtgcaa accccatctc atgtgttaca cacagcattg
      661 tcatacccga ctgcgccagc ccaatcatcg tatccagcac ctctttcacc atctcaggat
      721 cgagcgccga cgttggctca tcaaataaca taattttcgg cttcatacac agcgaacgcg
      781 caatggcaac gcgttgctgc tgaccacctg aaatctgtcc gggaaactta tgcgcatgtt
      841 cggcaattct cacccgctca aggtaatgca tcgccagagc ttctgcctct ttctttggca
      901 tcttgcgtac ccaaatcggt gccagggtac agttctgtag aacggtcaga tgagggaaga
      961 gattgaaatg ctgaaagacc attcccactt cctgcctgac gcgctcaata ttgcgaatat
     1021 cttcgttaag ctcgatgcca tctaccacga tccgtccctg ttggtgttct tccagatgat
     1081 taatacaacg aatggttgtc gatttaccgg aaccagaagg gccacacaga acgatccgtt
     1141 ctcccggttg cacggttaaa tttatatttt tcaaaacatg gaattgccca taccatttat
     1201 tgacgttttc cagcgtaatc atcgcgttag caggttgcag taaaatttgg ctcatagtgt
     1261 cctcaatgcg gtgtacgccc ggtgttaaaa cgtttttcca gatgttggct ataacgtgac
     1321 atgctgaaac agaagatcca gtagatcagt gcggcgaaaa catacccttc cgtcgacata
     1381 cccaaccagg cgggatcaac ggttgcctgc tgaacgctac tgaaaagatc gaacaacccg
     1441 atgatgatca ccaggctggt atctttgaag agtgcgatga tggtatttac cagcccagga
     1501 atcaccagct tcaacgcctg tggcagaata accagcccct ggtttttcca gtaacccaac
     1561 gccagcgact ctgccgcttc atattgccct ttaggcagcg cctgtaatcc gcctcgcacg
     1621 acttccgcaa catatgctga ctgaaacagg atcacgccaa ccagcgcgcg gatcaatttg
     1681 tcgatactgg tgccttctgc cataaacaac ggcagcatga ccgaagacat aaacagaacg
     1741 gttatcagcg gtacaccgcg ccagaactcg ataaaaatga ccgataagat acgcacaatc
     1801 ggcatatggg agcggcgacc taacgccagt aagatccccc acggcagcgc ccccgcaatc
     1861 ccaactgatg caataattaa agttagcgtc agcccgcccc attgccgggt ttcaacccgc
     1921 tcaagaccaa gaaacccgcc atacatcagc caccagacaa tcagtgggta aatcaccgcc
     1981 cataccgcaa tatagcgacc gcgatgcggg agtattttcc agaacattgg tgcgatcgac
     2041 acaagcccaa tcagtaatgc gaggttaatt cgccagcgtt ggtcgtgtgg gtaaagccca
     2101 tacataaact gaccaaatcg ttcgtggatg aagacccaac aggcaccggc ttttgtgcag
     2161 tcggtacgcg ttgagccaac ccagttagcc tgcaaaaatg cccagttcag caacggtgga
     2221 atcagttccc acatcaacca tatgcagcca atagtcagca ggctattgct ccagctggag
     2281 aacagatttt ttcgcaccca caccatcgcg cggcttgagt tatggctcgc cgggcgcggg
     2341 ggatgagaca gcaatacttt tgtcatcatg actccttagc gttcaacgat cgcgatgcgg
     2401 cggttataga tattcatcag cagcgagata gtcaggctga taatcagata gaccgacatg
     2461 gtcatggcta tcgtctcgat ggcttgcccc gtctggttca gcacggtgcc agcaaacagc
     2521 gaaaccatat ccggatagcc aatagcggcg gcaagagagg agtttttgac gatgttgaga
     2581 tactggctgg ttaacggcgg aataatcact cgcagtgcct ggggaataat gacctggcgt
     2641 agcgtaaccg gattgggtaa tcccagtgac cgagccgctt catgttgacc ataaggcact
     2701 gcctggatcc cagcgcggat aatctcggcg ataaatgcag aggtataaac cgaaagcgcc
     2761 agcgttaagg ctgccagttc aggaattaaa accatcccgc cacggaagtt aaagcctcgt
     2821 agggctggaa catcccagtg tagtgctgcg ccaaaaagcc attgcgccag taaaggcaaa
     2881 cctatgatca acataacggc gaccggccag gttcggcgca attgtccggt ctttatctgg
     2941 tacgttttat taaaacggaa tagtccaaca gaaatggcta tagcaataac aacagtcacg
     3001 ataaacgcaa taaatccgtc tcccagctgc ggagatggaa tataaagccc acgattgctc
     3061 aaaaaggcca gatcaaacgc actcactgcc tggcgtggtc cgggcaaatt gcgcaacacg
     3121 gcaaagtatc agaagaagat ttgcagcagc ggcggaatat tacggaagat ctcaatataa
     3181 attgtggaaa gctttcgtag cagccagtta tccgaaagtc gcgccagacc gataaagaag
     3241 cccagaacag aagcgaatac aatacacaat gcagaaacca gtagcgtatt gagtaagcca
     3301 acaatgaaaa cgcgtccgta ggtatcaccc tgctggtaat cgatcaaatg ctggacaata
     3361 ccgaagccag cgccgcgatc cagaaaggca aaacctgaag taatgccacg attactgaga
     3421 ttcgtcacag tgttgtgaaa tagccaacca acaattccga caacagcaac aacggcaaag
     3481 atctggaata accaggcgcg aaccgtaggg ttggcaaagg agagtgagcc tttaacggtt
     3541 gagcggcgat gagacataga aacctcggaa agcataacgc tgaagagagc gccgccgtag
     3601 cgacgctcgt tacctcacac cgacttaacg cacgggcggc gcgtactgaa taccgccgtt
     3661 attccagaga ttattttgcc cacgtttaat tttcagcggg ctttctgaac ctacgttacg
     3721 ctcgaaaatt tccgagtagt tacccacctg tttgatgatg ttaaaggccc atttattatc
     3781 cagcttcaga tccttgccgt aatcgccttc tttacccagc agatgtgcca tatcaggtgt
     3841 tgctggatta gccgcttttt catcgacgtt ctgggaattg atgcccatct cttcagcatt
     3901 cagcatggcg aaaagcgtcc agcgtacaat cgagaaccat tcatcatcgc cacgacgaac
     3961 taccggccca agtggttctt tagagataac ttccggtaag acaatccatt cagccgggtt
     4021 gcttaatttg atgcgcaggg catacagttg tgattgatcc gaggccagcg tatcgcagcg
     4081 accagattcc agtgccttcg ctgattcgtc agagcgatcg aaagtcactg gtgtgtactt
     4141 catattgttt gccttaaagt aatcggcaac gttgagttcg gtatcagtac ccgcctgaat
     4201 acagacggta gcgccatcca gttctttcgc gctttttagc cccgctttat cgtgcgtcag
     4261 gaagccaatg ccgtcgtaat aagtgacgcc agtaaatgcc attcccatcc cggcatcgcg
     4321 agatgaagtc caggtcgtat tacgggagag caaatccacc tcccctgact gtaaagcggt
     4381 gaagcgttct tttgcagtga gcggggtata tttcactttc gtatcgtcac cgaatacagc
     4441 agcggcaaca ccacgacaaa catctacatc aatacctgaa aacttaccgt cagcatcggc
     4501 ataagagaac ccaggtaatc catcactgat cccgcattgc acaaaacctt ttttctgaac
     4561 ggcatcaagc gtcgcgccag catgcgcctg atttgcaacg gcaagcagca cgctggcggc
     4621 agccagtgtg gctatcatca tctttttcat aatgcttcct gtgaggcgaa aattatcgtt
     4681 ataggacgtt tgggaaacgt cttttacctg tctgtggcca tcctcttata agcaaaagta
     4741 gtgccagctt ttgcgcacga tttcgcgtcg caataagctg agtgtagaca ggaaataata
     4801 aaaactgcgc cccgcaatgg tgcgaaaatg aaacatgcgc cacattttgg cgcaaaatat
     4861 ttacgcgttt tttactaagc agaataatga ttgttagcgt gagtatattt ctggtgggat
     4921 gtgtgaaagt taaattgatg cgttatttcg aaagcagtga aataacgtga tataacatgc
     4981 taaatcagca gggaattacc cagcagaata tagcaatatt ttatcttatg gaaagatact
     5041 gtatcgttaa aaagagcgcg gagctccgcg ccctgtttag ttatttcgac agagcgatga
     5101 tccccatcgt caggccagca acagctgctg ccccgccggc aatagcccca gccgtttccc
     5161 agttatcctg agtcgtgcct ttcatacagg tattggtgtg aaccagacga ccctgatcgt
     5221 cataaaccgg aacgcagggc gaatcgtgcg cacagcccgc cagcaatgcg gtgagcaatg
     5281 ccacaggaat taatcttttc atggtgttac ctcagtaata aacgctaacg cttaataagt
     5341 taactctctt attaacgagc tgaatgccat tttatcactg gaggttataa atcttgcgga
     5401 atgaataatg acaaattatg tgaattgtaa aaatagtgaa gggagcggga actatagaaa
     5461 gtttttaaag agtgagtaga ggaatatctt attaattttt atgggtttca ctggaaataa
     5521 taaaggcacc cgaaagtgcc tttatgtt
//
LOCUS       DAZGAP010000259         5521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGAP010000259 DAZGAP010000000
VERSION     DAZGAP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.260"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..810
                     /locus_tag="ACHLAM_004807"
     CDS             <1..810
                     /locus_tag="ACHLAM_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO7856207.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKLLNEAVLFSAPEGMALT
                     SGEDMQMSAARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGAVDVQ
                     AQNGSMRLFAEKKLTISSESDISFTGKKRITLIGGGSYLRLEAGKVEYGTMATCMRRV
                     KRTMTEAAQSMPMTFPRMSSSHIYSAIYRLLDKHGNILANTPCRLTTPSGQTVMGYSD
                     NEGRSVPVYTRQQEDVELHFFVKKPQPEETMWFVGETDTQQLETELRESQS"
     gene            810..2093
                     /locus_tag="ACHLAM_004808"
     CDS             810..2093
                     /locus_tag="ACHLAM_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO7856208.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGRNRTRMTILEIHDCRPEE
                     HQWVDQVVNVTMKAWQISGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKVVSDL
                     SEGAVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVKSW
                     TNTQIKAAWTEIQHVTDMTLDMLKQVDWVQVLTNVAKTVGVILVAVGVGGLVVTLGIP
                     EAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVS"
     gene            2106..3320
                     /locus_tag="ACHLAM_004809"
     CDS             2106..3320
                     /locus_tag="ACHLAM_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFO7856209.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNLGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGEAVEGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIRSIRYDGRNGLHFYSEYEGLQFEEASSMAWFARFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKHGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            3334..3687
                     /locus_tag="ACHLAM_004810"
     CDS             3334..3687
                     /locus_tag="ACHLAM_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO7856210.1"
                     /translation="MTVQAGERLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKL
                     TLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTMATYLRRTKRTMVAAPATMPLP
                     IPLLLDLYPPLNGQI"
     gene            4016..4471
                     /gene="tssE"
                     /locus_tag="ACHLAM_004811"
     CDS             4016..4471
                     /gene="tssE"
                     /locus_tag="ACHLAM_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7856211.1"
                     /translation="MSHHSPSLYETLYGNFTGGLDLHQVCEENQVIVSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTTILQGMPGTAHQLMRVLSDVLLKYEPRIKRVDVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQQFVDNSSYYHV"
     gene            4539..4844
                     /locus_tag="ACHLAM_004812"
     CDS             4539..4844
                     /locus_tag="ACHLAM_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856212.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRVFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            4899..>5521
                     /locus_tag="ACHLAM_004813"
     CDS             4899..>5521
                     /locus_tag="ACHLAM_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFO7856213.1"
                     /translation="MTSEVKLKTGGDPRSFPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTTGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFNRLQKTLRAMPPDDRDNLPLLYQTETFLKALSDTLARHELKQSSKVALPE
                     TMVKGYITRLENQPMQGEVSSPVTLPARALRSDAPDVQGHQPLPHSRL"
BASE COUNT         1397 a         1307 c         1535 g         1282 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa actgctcaat gaagcagtgc ttttctcggc accggaagga
      121 atggcgctga ccagcggtga agatatgcag atgagtgcag cacgaaatgt ggctgtcaac
      181 gccggggggg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagaag
      241 ctggggcttt ttgcccggac cggtcaactt agcctgaaat ccggggaagg ggcagtggat
      301 gttcaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac catttcgtca
      361 gaaagtgata tctcgtttac cggcaagaaa cgcatcacgc tgattggcgg ggggagttac
      421 ctgcggcttg aagccgggaa ggtcgagtat ggtacgatgg caacctgtat gcgtcgggtg
      481 aagcggacga tgacggaggc tgcgcagagt atgccgatga cattcccccg tatgagttct
      541 tcgcatatct actcggctat ttatcggttg ctggacaaac acggcaatat tctggccaat
      601 accccctgtc gcctcaccac tccatctggt cagacagtaa tggggtatag cgataacgaa
      661 ggccgctccg tgccggttta cacccgccag caggaggatg tggaactgca tttttttgtt
      721 aaaaaaccac aacccgaaga aacgatgtgg tttgtggggg aaaccgatac gcagcaactg
      781 gaaaccgaac tcagggagag ccagtcataa tggaaatgcc cgatttaccc gagacaccga
      841 catctgatat tcccactggg gcgaacaacc agccgtggga ctgcaccacg acgggtgtcg
      901 aaatggtcga aaagcagtcg cgtctgccaa ccacggaaaa ccgtcagtac ctgcatataa
      961 aggtggaata tgccatgcgt tttccgcaac tgtcggcaca aatccgccgg aaaggtaaaa
     1021 cttaccagat gagccttaag cagaccgtta tgagtggtct tattcgcgtg gatgaaatcg
     1081 cccgcaatta ctgctggtat tataaggcgg aggtggcgtt tgatatgcgc accacgccgc
     1141 cacgcccttt tctgagcagt acgctgctgg aaaaagacgg tgctggccgt cgccacacga
     1201 ccaatccttt ccctgtgccc ggcaggggca tttaccgccg cccggacgtg attattgtta
     1261 aaaataagga agaccgctgg cccggactgg cggggccgga taccgaaggc aatatgcaca
     1321 gcgataacct ggcccgcctg gtggaggtga agtttccggg agataggctg tatgaagacc
     1381 aatatgaaga gtatatgcag attgtgggca ggaaccgaac ccgcatgacc attctggaga
     1441 ttcacgactg tcgcccggaa gagcaccagt gggttgacca ggtggtcaac gtgacgatga
     1501 aagcctggca gatcagcggc gcgaaatact ggccgctggc tctttacccg ccagtcttta
     1561 tgccccgtcc gccatcaacc cccgttgctg ccagaataga gccctggact tacgctggta
     1621 aagtagtatc tgacctcagt gagggggcgg tgcgtggtgt ggctgaaggc tggaatgccc
     1681 tgtcaaaaga gacgcaactg gtcctgaatt cagcagccag ctggcttaac gacagcggtg
     1741 agtgggttta ccggcaggga agtgaggtct gggtctgggc cagccaaacc ggtaagtcgg
     1801 tgaaatcctg gaccaacacc cagattaagg ccgcatggac agaaatacag cacgtcaccg
     1861 atatgacgct tgatatgctg aaacaggtgg actgggtaca ggttttaacc aacgtcgcga
     1921 aaacggtcgg cgttattctg gttgctgtgg gtgtcggcgg actggtcgta acgctgggta
     1981 tcccggaagc cattattggc gcgtttttac tgattgttcg tctggcaata agcgcctggg
     2041 gaacactggc gacgattctc ggtacgggta cggcggtact ggcagttagt taaacaggag
     2101 caggtatgga attgcatgat tttgagctga actacatcga gaaatggctg ccggaagcct
     2161 ccgtgaaata caaagacggc agtccggcag tcaatctcgg tctgattatc accgtctttt
     2221 ttaaggacgg acatacgcca gaagtccgcc gcaggatggt ggagtgcgtg gatcgcttct
     2281 atggggagtt taaaccctgg ctgaaaaaaa cgatcaccaa aaactgggtt ggcatcactg
     2341 ataataacta cgccaaaaag cggcaggaga tcctcgattc aacgcctgaa gagatttttt
     2401 cctggtatct gagtagcgca gagaaggatt ttttagcccc cgattacagt attttaatta
     2461 tggggaaaag aatttttcat aatgacaata atcgttcagt cattaagtta acactcccgt
     2521 tgtcactgct gaaagaaact gatggaaagc agcgttatga agcctggctg atgtggttgt
     2581 gtgacacctt ttcggtggaa agtggttatg cagggttgtc gtttgcactt ccttatgccc
     2641 gtgagcgaat gttcccctgt gaattcgccc tggcgcagcg tttttccggt gtgatggtgg
     2701 attctcttgg gacgcttgaa ggcggtgaag ccgttgaggg gctgaaaggt gcctgctggt
     2761 acaccattct cggtacgccg tggctggaaa aactgggggg ggcagagaga gtaaaacatc
     2821 gtttagctaa aacgcctgaa attagcctgc taccctataa caatggtgtc gtacttaaag
     2881 ccggtgaact ccccccgcca ctgggagaga tgaaaacaga aggtttatcg ccgctgctgg
     2941 tgaaggtgaa ccaggtcatt cgttccattc gctatgatgg acgcaatggt ctgcattttt
     3001 actcagaata tgaaggactt cagtttgaag aagcgtcttc aatggcatgg tttgcccgtt
     3061 ttgacgaagc cagtgccttg ctcgataaag aagaaatggg gaaatcttgc gcccccgaac
     3121 gcattaaatg ctggaccgat gaaatagcgc cccatgccgg acaatgggcc gcgatggtta
     3181 atggcaccac cgagtacatc cagacccgcg aagggcagaa aatgcccccg tttgaggata
     3241 agcacggtaa gcagcacagg gcttgctgga aactactgaa acgagatgac aatggtagcg
     3301 tttatacact acctgagtaa ggtcgtgtgc tgaatgaccg tgcaggccgg agagaggctg
     3361 gggctttttg cccgcaccgg tcaacttagc ctgaaatccg gggaaggggc agtggatgtt
     3421 caggcgcaaa acggcagcat gcggttgttt gctgagaaga agctgacgct gagctcagcg
     3481 agtgacatct cctttgccgg gaaaaaacgc atcacgctga ttggcggggg aagttaccta
     3541 cggctggagg cggggaaaat agagtacggt acgatggcga cgtatctgcg gcggacgaaa
     3601 cggacgatgg tagcagcccc ggcaacaatg ccgctcccca taccgcttct cctggacctg
     3661 tatccaccac ttaatggcca aatttgaatt gaatgtctcc tcaatgccat caaagtcaac
     3721 gatgctatcg ttcagggagc gtagtcaatg gatgtcacaa aaatgatgca ggtgctacag
     3781 gatttgtttt tatgctgcta aatgtacagc gacttcgtga tatggggttg ttggtgtttt
     3841 ttataataat gctttttacg gaatcaaaaa aacagacaac atattaatta tttgagaaag
     3901 cagcgtcaaa aaggaaaagt taaaatgtcg aatcgatttt ttactccggt aatcttcttt
     3961 ccagtgtgtt gattgaacta tttccaagat taaatgatta tgagaagaag tcgtgatgtc
     4021 tcatcatagc ccctcccttt acgaaacctt atacggaaat tttaccggcg gcctcgacct
     4081 ccatcaggtc tgtgaagaaa atcaggtcat cgtgtcggtg ctcgataaca tgcagcgtat
     4141 tctgaatacc cgggccggaa gccttaaaca tcttccggac tatgggctgc cggatatgac
     4201 aaccattctg cagggaatgc cgggtacggc gcatcaactg atgcgcgtgc tttcagatgt
     4261 tctgctgaag tatgagccac gtattaagcg tgtcgacgtg acgatgcagg aacagactca
     4321 gcctggtgag ttgcattatg tcattgatgc agaacttaaa gatgccgggc tggttcgtta
     4381 cggaacaaca tttatgccgg agggcagagt gttgctgcgc catcttaagc aacagcagtt
     4441 tgtggacaat tcatcttatt atcatgtctg atttttttat ccgggctgcc cctgtgtggg
     4501 tggtccggat gaaaaatata ttttttgtga ttttgattat gaacccgtta tttaacgata
     4561 ttcagatgcg tcttttttat ctgaaccact ctccgtactc ctggcactgg aatgtcagat
     4621 tcagacctca ggaagccgta tatattggca gcgatacctg tcatatcacc attacctgta
     4681 atcagtccgg ttttcattta acccgggacg gacaacgggt attcactgag cgttatatca
     4741 gaaaccttaa tgagctgtta ccagttctga aacggcggtg ggatgtgaca ccggcaatca
     4801 tccgtgcagt ggaatacctt tcccgggtcc cggtatcaca ctgacattct ttccgtaact
     4861 gttaatcatg atttaataca gcaacactga atctgccgat gacttctgaa gtgaaactga
     4921 aaacaggtgg tgacccccgg tcattcccgg attatgctgc cctgcgcgat gaaatcagca
     4981 aactgaccca cccggcacgc ccggatgtgg actggcgtta tgtggaaacg ctctgcctgc
     5041 gcctgtatga acacaatggc gtggaattac agacagcatc ctggtacacc attgcccgga
     5101 tgcatacaac agggttaagt ggcctgaatg aagggctggc tctgattgtg gcgctgaccc
     5161 ggcatcactg gtcagtgatg tggccgctga atacccacgc ccggctggaa attattaccg
     5221 gtctgttcaa ccgtctgcaa aaaacgctcc gggccatgcc accggacgac cgtgacaacc
     5281 tgcccctgct gtatcagacc gaaacattcc tgaaggcact gagtgataca ctggcccggc
     5341 acgaacttaa acagagcagt aaagtggctt taccggaaac catggtgaag ggatatatca
     5401 cccgtctgga aaatcagccg atgcagggcg aagtgtcgtc tccggtaacc cttcctgctc
     5461 gggcactccg gtctgatgct ccggatgtac agggacatca gcccctgccg cactcccgtc
     5521 t
//
LOCUS       DAZGAP010000260         5392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGAP010000260 DAZGAP010000000
VERSION     DAZGAP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.261"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(167..352)
                     /locus_tag="ACHLAM_004814"
                     /pseudo
     CDS             complement(167..352)
                     /locus_tag="ACHLAM_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
     gene            complement(506..1663)
                     /gene="intS"
                     /locus_tag="ACHLAM_004815"
     CDS             complement(506..1663)
                     /gene="intS"
                     /locus_tag="ACHLAM_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO7856214.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            complement(1825..1899)
                     /locus_tag="ACHLAM_004816"
     tRNA            complement(1825..1899)
                     /locus_tag="ACHLAM_004816"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1864..1866),aa:Arg,seq:cct)
     gene            complement(1975..2907)
                     /gene="yfdC"
                     /locus_tag="ACHLAM_004817"
     CDS             complement(1975..2907)
                     /gene="yfdC"
                     /locus_tag="ACHLAM_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO7856215.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKTIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3201..3956
                     /gene="mlaA"
                     /locus_tag="ACHLAM_004818"
     CDS             3201..3956
                     /gene="mlaA"
                     /locus_tag="ACHLAM_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO7856216.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4018..5358)
                     /gene="fadL"
                     /locus_tag="ACHLAM_004819"
     CDS             complement(4018..5358)
                     /gene="fadL"
                     /locus_tag="ACHLAM_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO7856217.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1345 a         1301 c         1274 g         1472 t
ORIGIN      
        1 tttcagttac tctatcgaat tgataaatat aataaaccta gtgttaatta ttatcactag
       61 gtttattttc tcactcgcat ttcttactaa aatcaattac aaatacagtt ccattatatt
      121 ttatgtcgta taatgctgta gaggatatgc tatcaaatga tcaataagtc gatgctttga
      181 atgtgaattt tgcattcgcg aagaagctaa aactcatagc cacaacgaaa cctgcgaagt
      241 tcgccatagc ttgatttgta tgcgctgcat ggatacaaac accaaaaaac acccagtgta
      301 aaagggtgtt cagcacacca atagaggtgt actttgcaaa tagctttaac atttcttttg
      361 tcagctaata atcaaaggca tgaagtctat catccaagtc tcaatcgatc gatgacttgc
      421 tatggttgat gagacaaaac tgagacaaac aaagctttgc actggattac aaggctttgt
      481 gatattcgat agttgttaag gcggatcact ccaccttttc atcaatccag tccgcccacc
      541 attgcatcat ttctctgcgc ttatcgagat actgtgcatg gttgtaaatc ccgcgcacag
      601 atccgccgtt ggcatgtgcc agttgcactt caatagcatc agcaggccat tcgtgctcgt
      661 tcataatcgt gctgaattca tgcctgaatc cgtgaccgct ttccagacct tcatagccga
      721 tttgtttgat cacaagtagc accgcgttct cgcagattgg ctttttctta tcgttgcgtc
      781 cggcaaaaac aaactctgat actggtttag tgatggagct tagcgtagtg agaagttcaa
      841 ccacctggtc cgacatcgga accacatgaa tcttgcgtcc cttcatcaca ttggcgtcga
      901 tggtgataat cctgttttca aaatcgacgt tcttccatag catggaacga agctcttttg
      961 ttctgagggc tgtgtagcgt aaaactttgg tcgcaatgag cgatacgata cttcctgaaa
     1021 atgttgccag tgctttgtta aatgccggga tctggtctgc aggaagaaac gggaagttct
     1081 tcttgcggta tcccttcatg gcgtcagcaa ggtcaggtgc cgggttatat ttagcccttc
     1141 cggtgacaat agcgtaacgg aaaacctcgc cgcatcttct tcgtgctttg ttggctcgct
     1201 ccattgcacc gcgatcttca aatctgcgga ttacttccag cagttgcatc ggctcaatat
     1261 cctgaatttc aaggccgccg atgataggta aaatgtcgtc atcaaacatt tttgcaagtt
     1321 cagtcgcata ccctacagac cagacttgct tcttgtgctc gtaccattcc ttgtaaatgg
     1381 cgctaaagga attgttgtta gacgaagcct ttttcgcctt taccggatcg atgccaaccg
     1441 agatgtcttt tcttgcggtc catgccttat cccttgcttc ctgcaaagtc ataagcggat
     1501 attttccgac agtcaggatt ttctccttac cgtcaatctt gtagcgaagc tgccatacct
     1561 ttttccctga cacagggaca taaaggtaca ggccattacc atcgagaagg cgatatggtt
     1621 tttctttcgg ctttgctgct tcaatctgct taacggtgag catgggtaaa aatccggggg
     1681 gtaaaattat tttatccact ttttacccgt catggagtgc ggctgtcaac gatctgacga
     1741 gaaccatgac gaactgtgaa tctacggaag gcttgatatt cagggtattt tgcggactgg
     1801 tacggatggg agcgaactga taaatggtgt cccctgcagg aatcgaacct gcaattagcc
     1861 cttaggaggg gctcgttata tccatttaac taagaggaca atgcggcatg agtatacccg
     1921 ctaatggact gcggggtaag tacgctgccg ctcgattgct taaaccctcg ccatttatgc
     1981 cgggttttta tcatttttct taatgttttc cgcacgttct gctttttggc gtgcttctgc
     2041 tttgcgcttg ttgctcatgt cgttacggat ctgtgcatga ctcattaacg cgaagataaa
     2101 ggtgccgccg cagatgttcc ccgctaaagt aggtagtgcg aagggccaga tgaaatcgct
     2161 ccagtgcagc gtaccgttaa acaccagata gaggatttca acagaaccga caacgatgtg
     2221 agtggtgtca cccagggcaa taagccaggt catcaatata atcaccacaa tctttgccgc
     2281 acccgctgca ggaaacatcc aaaccatagt ggcgatcagc cagccggaaa tgatcgcgtt
     2341 ggcaaacatc tcgctggggg tgttcttcat cacatccatg ccgattttga caaatgcatc
     2401 gcgagtttct tcattgaaga ttggcatata ttcaaatgcc cacgccgcaa tacctgtccc
     2461 gagaatatta cccagcagca cgacgcccca taaccgcata agtaagccaa cgttgctcat
     2521 tgtcggtttt tgcatgacgg gtagtaccgc agtcacggtg ttttcggtaa ataattgctg
     2581 gcgggccata atgacgataa taaaaccaaa ggtataaccg agattctcca gcaagaagct
     2641 gcccggcacg ccttccagtt cgacatgaaa tatccctttt gccagtagcg aagcgcccat
     2701 cgacagaccc gccgcaatgg ctgaccacag tagtgccatt gcgtcgcgtt ccagctcttt
     2761 ttcaccatcc tggcggatat gctcatgaat tgccatcgcc cgggagggga gtcgatcttc
     2821 atctatttct attgttttgc cgcgctcttt ttcttcgctc tcaacttcaa tttcgtcgct
     2881 gtgttgatca attttgtcgt tgtccatggt ctcttctctt gaattagcac gtatagctaa
     2941 agcgtagcgg cttttttgct cgcaactggc gggagttact ctgaaaatgt agaaaaggct
     3001 gcgtttgcct ttttctgttt ctatagaatc aagtagccta cagggcggcg attaccaggc
     3061 tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt gacgtgctgt tacaatcgcc
     3121 cacacctaaa caggcggata cggtatcgtt ccgtcatgga tggcaaactg cataagccat
     3181 aaaaaaacag ggagacattt atgaagcttc gcctgtcggc gcttgctctg ggaactacgc
     3241 ttctggtggg gtgtgcgagt tccggtacag atcagcaagg gcgttctgac ccgctagaag
     3301 ggttcaaccg caccatgtac aacttcaatt tcaatgtatt agacccgtat attgttcgac
     3361 cggtcgctgt cgcctggcgt gattatgttc cgcaaccggc gcgtaacggt ttgagcaact
     3421 ttactggcaa ccttgaagaa cctgcggtga tggttaacta cttcttgcag ggcgaccctt
     3481 atcaggggat ggtccacttt acccgctttt tcctgaacac cattttgggg atgggcggct
     3541 ttattgatgt tgcagggatg gcgaacccga aactgcaacg gactgaacct caccgcttcg
     3601 gtagtacgct tggtcattat ggtgtgggtt atgggccata cgttcagtta ccgttctacg
     3661 gtagcttcac gctgcgtgat gacggtggtg atatggcgga tggtctttac ccggttcttt
     3721 cctggctgac ctggccgatg tctgtgggta aatggacgct tgaagggata gaaactcgtg
     3781 cgcagttgtt ggattccgat ggtctgctgc gtcagtcgtc cgatccttat attatggtgc
     3841 gcgaagcgta cttccagcgt catgatttca tcgctaatgg cggcgaactc aaaccgcagg
     3901 aaaatccgaa cgcgcaagcg attcaggatg atttaaaaga tatcgattct gaataagaaa
     3961 caaacaaaaa aggtgagtcg caaaactcac cttttttctt attaagcgaa cgcgttatca
     4021 gaacgcgtag ttaaagttag taccgaacag ccaggcttta ccttcagact cgaactggta
     4081 tgggccttcg ttaattttca cgctctgacc gtgcatataa gaaacaccaa catcgactga
     4141 agcatcttta ttaaatgcgt aagtcgtacc tgcactcagc cagaaacggt cctggtccgg
     4201 aatggagata gaacgattct gtgccggaac tgggctgtca tcaaaggcga taccggtacg
     4261 gaaggtccag ttatcatcgt agtaataagt ggttcccaac gcgatgcggt aagcatcttt
     4321 aaagccttca tgtttctgga acagcgtgtc gccactcgtt gaggtcgctt tcagctgctg
     4381 gaactgactc cagctggtgt aagccaggct atagtgaatt gcccactgcg gatcaacacg
     4441 gttataaccc gacacttccc acatttcagg caggttcagc gtcagataac ccgactgcgt
     4501 tgcgccacct gtcgcggtag gaattggcaa accgtagtta ttaaaagccg ggttaagatc
     4561 gctgctgtag ttacccttga agtcaatttt cacttcagag cggtaggtca atgcgtagcg
     4621 gttgttttta tccagttcat acaggatacc ggcgttccag ccaaagcccc actggttacc
     4681 gttcaggtga gcgattttgg tattactgtc gatgccgttg gcggtagctg ctaatgcttg
     4741 cccctgagga gtcagccaag caggagattg cctaatctga ccagccacca gttgccccag
     4801 atcgcctgcg aaacgttcaa ttttcgcgcg agcgtagacg gcgttgaaac caagaccaaa
     4861 gctccatgca ttatttaagc gatatgcacc gcttaagttc aggttcatgg tttcgaggtc
     4921 ggtggtacca ccgacagaac cgcctgcata agtatcgtta aactctgtcg ccagaccata
     4981 gttagaggta attgaagccc cccaaccaaa ttggtcgtta atcggtgcaa caaagtgcat
     5041 gttcggaacc catgccgtag gcgcgatgtt atcggctttc aggctacgac cagacggaga
     5101 cgttccgctg atatttacat ccggatcaat ataaaccgca cccgcagaaa atgtcgggcg
     5161 gtcaaacatg gtaatcaatg cggggttacg gctaacgtta cctgcatcat cggcaattgc
     5221 accttcccct gaataagccc ggcccaggcc agaggaagaa aattcgttta actgaaagcc
     5281 tgccgaccag gcctgggtgg agataagtgc cactgcgact gcgagagcag actttgtaaa
     5341 cagggttttc tggctcatga ccataacctc aatgatttat ttttatacaa ac
//
LOCUS       DAZGAP010000261         5261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGAP010000261 DAZGAP010000000
VERSION     DAZGAP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.262"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(63..1763)
                     /gene="ltrA"
                     /locus_tag="ACHLAM_004820"
     CDS             complement(63..1763)
                     /gene="ltrA"
                     /locus_tag="ACHLAM_004820"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO7856218.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWVRYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            2385..2996
                     /locus_tag="ACHLAM_004821"
                     /pseudo
     CDS             2385..2996
                     /locus_tag="ACHLAM_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            2997..3218
                     /locus_tag="ACHLAM_004822"
     CDS             2997..3218
                     /locus_tag="ACHLAM_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856219.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            3178..3664
                     /locus_tag="ACHLAM_004823"
                     /pseudo
     CDS             3178..3664
                     /locus_tag="ACHLAM_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            3709..4479
                     /locus_tag="ACHLAM_004824"
     CDS             3709..4479
                     /locus_tag="ACHLAM_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856220.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDISRLATLLISRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVAEDTRRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            4894..>5261
                     /locus_tag="ACHLAM_004825"
     CDS             4894..>5261
                     /locus_tag="ACHLAM_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7856221.1"
                     /translation="MQYAKPVTLNVEECDRLYFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQ"
BASE COUNT         1073 a         1437 c         1434 g         1317 t
ORIGIN      
        1 tactgtgcag ccgcccccta agaaccgtac gtgccagttt cccggcatac ggctcaagcc
       61 tttcaataac tccacattag agccggcttc ataacagata ttccctgatg ttttgcagtg
      121 tgatgacatg ccggatgtaa catggccaga ttacctatgt tatccagtcc cccttcggat
      181 ttcggcacaa tgtgtcgaag ttgccagcct gttgtggcgt tcactggcag gtggcagacg
      241 gggcactgac ctccctgtcg gagccacaga tttcttaggc gagagtaacc ctgtatggat
      301 tccagcatcg tagcttttag ccgtgcttca aagtacgatt cgtagctggg atcaaaaggg
      361 ttagcagtgc tttttatttg tctgtgtctt ttgactggca tatccgagat ttcatatagc
      421 gccagtaact cacgtttgcc tgatggcagg agcttccctg tttctgtgcc gaatacccag
      481 tgtcggaagt taactgaacg aaagtatttc tttcttatcc agcctgtacc tttctttggg
      541 tgtcgtcgac atgcccagcg ccatagtgct ttccatattt catgactgac tttctgaaag
      601 atcttttttg aaacaacatg acgatagtat ctgacccagc ctttgaggac tgggttgaga
      661 tgccatatca gtgtgctctg gctgactgtc ggattgtctt ttatcgtctg gcggaccctt
      721 ttcaggaatt cctttacctt ttggggtgaa ggtttaatca acagtttccc gttatatttg
      781 cggatgttca tacccagaaa gtcgaatcca tcactgatgt gtgtgattcg tgtcttttct
      841 ggtgagaggg taagccctct ttctgccaga aagttttcta acaagggaag aaccttttct
      901 gacagcgtgt cgtggcttat ccccgtgatc acaaaatcgt ccgcataacg gattatgttg
      961 atcttttctg gattcagtcc gcctcgatag aaggttttct tcaacagagg ctgcagacca
     1021 tccagaacca tgtttgccag cgttggagat attattccgc cctgtggagt tcctgcactg
     1081 gttgggtata atttccccaa atcaatatat ccggctttca gccatcgttt caggatcgcc
     1141 gtatccgttg ggatattggc gattagccat tcatgtgaaa tctcatcaaa acaactgcga
     1201 atgtcggctt ccagaatcca ttgtgcccga tcagccctgt gcaacgttgt gaaacattgc
     1261 tcaatggcat cggctgttga gcgacagggc ctgaacccaa acgaatttgg gtcagccgtt
     1321 gtttctgcaa caggttccag cgccatcaga tagagtgcct gcatggctct gtctttcatt
     1381 gtcgggatcc ctaatggtcg ctttttgccg ttagattttg ggatatatat acgccgtagc
     1441 ggaagaggtt tgtaacccct tcttttcagc gtgtatatag ccttcatctt gctgccgggt
     1501 tttgaccatg ttttgccatc aacacccggc gttctcttcc ctctgttatc tgtcactcgt
     1561 ttaactgcca ttgctctggc gctgaacgag tgggtgagta gccattgcaa ggcttttacc
     1621 ttgcccgctt tctcctcctg tattgccttc acgatacgcg cctgcagcct tctgacctcg
     1681 cgacggcact tggcccagtt gatattgtgc cagtgagtca cgatgtcggt gggtgcaccc
     1741 gcgtttatgt ttttcgcagt catctgctta ccctccttca aaggtattca ggttatctcg
     1801 tgttgaaaga ccatgcggaa gtctgcccgc tttcgcgtgg aatgatgttg ctgaaacagc
     1861 tcaactcctg tccatcccat tacaggatgg cattcgcttt ctccgcactc ctgtacccgc
     1921 atcactgaca gctttcctcg cggttagcct gccctgaggg cagtgatacg ggcttaccgt
     1981 gttccgcaca gtttgcaggg atgggttagg ttctgcctat ccaccggtag ctttgatgtc
     2041 cgtgcctccc cagtcaaaag aggaaggtcc agctacgttc ccattttggg tcgggcctgt
     2101 cagtgccttt ggcccgttca gggttgacga tgtttatcag cagttcacat gtgttaaccc
     2161 tgccatccag ccttgcgccc catccgccct gacactggca gacatgcttc ctcctcgcgg
     2221 attccacatg gtgtgaaaac cgggctacgt tgtcccgaag gcttcatacc gaacagttac
     2281 ccgctctgca tgcttcggta ggctacaggc gacggaacgc ctggttttac tacttccttt
     2341 acatgaagtt aaaacaacaa actgtgcgac tttcacgtcg cacactgact gacccgccgt
     2401 acctcgtcgg ttttcgtgac cgtttcgggc gcaccatcgc cggcgataaa accgatgaat
     2461 ggctgcaacc ggcctgtaat gaaatgtacc gcgtactcaa aaaagacgcg ctgatggtca
     2521 gcttctacgg ctggcaccgt attgaccgct ttatggcggc ctggaaacgc gccggattca
     2581 gcgtggcagg gcacctcgtg ttcaccaaaa actacacatc caaatctgcg tatgtcgcgt
     2641 accgccacga atgcgcttac atcctggcaa agggcagtcc ggcccggccc cgtaaccctc
     2701 tgccggatgt gctgggctgg aaatattcag gcaatcgtca tcacccgacg gaaaagcccg
     2761 ttaccagcct gcaaccactg attgagagct tcacacatcc gaacgcgatt gtgctggacc
     2821 cgtttgcagg cagcggctca acctgcgtgg ccgccctcca gtccggacgc cggtatatcg
     2881 gtatcgagct gcttgagcag tatcaccgtg ccggacagca acgtctggct gccgtacagc
     2941 gggccatgca gcagggggcc gcaaatgatg actggtttat gccggaggct gcgtaaatga
     3001 actatgccgg acacgaaaaa ctgcgcgccg aagtggcgga ggtggccaat gccatgtgcg
     3061 acctgcgtac aaccatgaat gagatggagc agcggtacag ctttaatgcc gacaccctgc
     3121 cggaacgtct ggtgcgtcag acgctgtttc gcgcaaaccg cctcctgatg gaggcatata
     3181 ccgaaattct ggaactggat tcctgcttca aagattgagg ggagagagta tgtacggaac
     3241 atgcgaaacg ctctgccggg cgctggcagc aaagtatccg ggagacacgc cgttgatgct
     3301 ggttgtctgg tccccggaag agattcaggc cctcgctgac ggaatggata tttccctgtc
     3361 cgatcatgaa atcaggaccg tcctggtgcg cctggaggac atcccggaag accagcggac
     3421 tgagtccggt atttcttccg gcgtggcgat ggagattatc agcaacgtga gcgaaaaccg
     3481 ccaggtgacc gtcccggctg aactgctggc gtccctgatt cagaccgccg aacaggcact
     3541 gtggaaacgt gagtgggccg cccgtgataa cggcctcgtc gtcccggaat gcgtcacccg
     3601 ccgtcaggcg gtggttaatc aggcccgcgc cctgctgaaa aacaacacac acgaaaacga
     3661 ctgatgttat cgccgccttc gggcggcgat tcagggagac cattaatcat gaacaaaacg
     3721 ctgaatgcac tggtttgtcg ccacgcccgt aacctgctgc tggcgcaggg gtggccggaa
     3781 gagacggata ttgaacaact gaatccgcac tatccgggct ggatcagcat ttatgtgcga
     3841 ctggatatat cccggctggc gacgttactt atcagccgcc atgatggcgt actgccgccg
     3901 cacctggcct ccgccattca gaaactgacc ggaaccggag cggaacttgt tctgtccggc
     3961 agccagtggc aggcgctgcc ggtacttccg gcagacggaa cgcaggtgtc tttcccgtat
     4021 gccggtgaat ggctgacgga ggaagaaatc agggcagttc ttgatgcggt gcgcaatgca
     4081 gtacgaagca tctgttacca ggtggcagaa gatacgcggc gtatccgggc ggcgctgacc
     4141 accaccggtc agacgttgct gacccgccag acgcgccgct ttcgtctggt cgtgaaggaa
     4201 agcgattacc cctgctggct tgatgaagat gacgaaaatc tgcccgtggt gctcgatgcc
     4261 attctgaacc gtggcgcccg tttttcggcg gtggaaatgt acctggtctg tgagtgtgtg
     4321 gagcatatcc tggccagtgg tctggtctgt gatgtgctgc gtataccgga tgaaccgtcc
     4381 cgtcgctggt ttgacaggga tattctgcgg gaggttgttc tggaggcacg ggatgaaatc
     4441 cgcagcatgg cggatgcact ggcgaaaatc cggaaatgat gacgctggcg gggaaaaatc
     4501 tccgcctttt ccagccggtc cccggacaga gcaaaaggat gtcgccgcag gctccgcttt
     4561 acccggccat gcggggcatg gccttgtggg ttttcagtta tgtggcctca gcgtcgtgtg
     4621 cgggctgtgc cgtgcctcca tcttagccgg gctggcgggg atgcaagggt aaaatgcacg
     4681 cggcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt cacccgctca
     4741 gatttcgcgg tctcaccctc caactcccgt cagccgtgtg cgtcaggctg cggcttccct
     4801 tgcgcccttg catccccgcc ttatcgcccg gcttttctgg aggcacggca ccgcccggtg
     4861 tcgcggaata tgtgaacaac ggaggaaaac gtcatgcaat atgcgaaacc tgtcactctg
     4921 aatgttgaag agtgtgaccg tctctacttt ctgccttacc tgtttggcaa cgattttctg
     4981 tatgccgagg cgtatgtgta cgcgctggcg caaaaaatga tgccggaata tcagggaggg
     5041 ttctggcatt tcatccgcct gccggacggt ggcggttaca tgatgccgga tggcgaccgt
     5101 tttcatctgg tcaacggtga aaactggttt gaccgcactg tgagtgctga tgccgcaggt
     5161 atcatcctga cttcccttgt gattaaccgc cagttgtggc tgtaccacga cagcggggat
     5221 gcaggactga cccagcttta ccggatgcgc gatgcgcagt t
//
LOCUS       DAZGAP010000262         5203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGAP010000262 DAZGAP010000000
VERSION     DAZGAP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.263"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..518
                     /gene="creA"
                     /locus_tag="ACHLAM_004826"
     CDS             45..518
                     /gene="creA"
                     /locus_tag="ACHLAM_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO7856222.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            531..1220
                     /gene="creB"
                     /locus_tag="ACHLAM_004827"
     CDS             531..1220
                     /gene="creB"
                     /locus_tag="ACHLAM_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO7856223.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLTLHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            1220..2644
                     /gene="creC"
                     /locus_tag="ACHLAM_004828"
     CDS             1220..2644
                     /gene="creC"
                     /locus_tag="ACHLAM_004828"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO7856224.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDTTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPTDPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            2702..4054
                     /gene="creD"
                     /locus_tag="ACHLAM_004829"
     CDS             2702..4054
                     /gene="creD"
                     /locus_tag="ACHLAM_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO7856225.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFIAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(4113..4829)
                     /gene="arcA"
                     /locus_tag="ACHLAM_004830"
     CDS             complement(4113..4829)
                     /gene="arcA"
                     /locus_tag="ACHLAM_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO7856226.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            4925..5065
                     /gene="yjjY"
                     /locus_tag="ACHLAM_004831"
     CDS             4925..5065
                     /gene="yjjY"
                     /locus_tag="ACHLAM_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO7856227.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
BASE COUNT         1234 a         1225 c         1417 g         1327 t
ORIGIN      
        1 gtgacgaact aattgctcgt gtaatagata aaaatggtaa caatatgaaa tacaagcatt
       61 tgatcctgtc tttaagcctg ataatgctgg ggccattggc tcatgcagaa gagattggtt
      121 cggtcgacac cgtatttaaa atgatcggcc cggatcacaa aattgttgtg gaagcctttg
      181 acgatcccga tgtgaaaaat gtcacctgtt atgtgagtcg ggcgaaaacc ggtggtatta
      241 aagggggatt gggcctggca gaagatacct ccgatgcggc catttcttgt cagcaagtcg
      301 ggccgattga actgtcggat cgtattaaaa acggcaaagc tcagggcgag gtagtattca
      361 aaaaacgcac gtccctggtc tttaagtcgt tacaggtcgt gcgcttttat gacgctaaac
      421 gcaacgcgct cgcttatctg gcttactccg acaaagttgt agaaggttcg ccgaaaaacg
      481 cgattagcgc ggttcctgtc atgccgtggc ggcaataaca gaggcgattt atgcaacggg
      541 aaacggtctg gttagtggaa gatgagcaag ggatagccga cacgctggtc tacatgttgc
      601 agcaggaagg ttttgccgtc gaggtctttg agcgaggctt gccggtgctg gataaagctc
      661 gccagcaggt tcccgatgtc atgattctcg acgttggttt gcctgatatc agtggctttg
      721 aattgtgccg ccagttactg acgctccatc cggcgttacc tgtactgttc ctgaccgccc
      781 gaagtgaaga ggtcgatcgc ctgcttgggc tggaaattgg cgctgacgac tatgtggcta
      841 aaccgttttc accccgcgaa gtgtgcgcca gggtgcgcac cttactgcgt cgggtgaaga
      901 agttctcgac gccgtctccc gtcatccgta ttggtcattt tgaattgaat gaacccgcgg
      961 cgcagatcag ctggtttgac acgccattaa cgctgacgcg gtatgagttt ttattgctga
     1021 agacgttact caagtcgcca ggccgcgtct ggtcccgcca gcaactgatg gatagcgtgt
     1081 gggaagatgc gcaggacacc tacgatcgca ccgtcgatac ccacattaaa acgctgcgtg
     1141 ccaagctgcg cgccatcaac cccgatcttt caccgattaa tactcatcgc ggcatgggat
     1201 atagcctgag gggcctgtaa tgcgtatcgg catgcggttg ttgctgggct attttttact
     1261 ggtggcggtg gcagcttggt tcgtactggc catttttgtc aaagaagtta aaccgggcgt
     1321 gcgaagagca acggagggga cgttgatcga caccgcaacg ttgctggcgg agctggcgcg
     1381 tcccgatttg ctctctgggg acacaacgca tgggcaactg gcgcaggcgt ttaatcagct
     1441 acagcatcgc ccgtttcgcg ccaatatcgg tggcattaac aaagtgcgca atgaatatca
     1501 tgtctatatg accgatgcgc agggcaaagt attgttcgat tcggcaaata aagccgttgg
     1561 acaggattat tcgcgctgga atgacgtctg gctaacgttg cgtggtcagt atggtgcgcg
     1621 cagcacgttg caaaatccta ccgatcccga aagttcggtg atgtatgttg ccgcgccgat
     1681 tatggacggc tcgcggctta ttggcgtttt gagcgtaggc aaaccgaacg cggcgatggc
     1741 tccggtcatt aaacgcagcg agcggcgaat tttatgggcc agcgccattt tgttggggat
     1801 tgcactggtg attggcgcag gcatggtttg gtggatcaac cgctccattg cccggctcac
     1861 tcgctatgct gattccgtca ctgacaataa gcccgttcct ctccccgatc tcggtagtag
     1921 cgagctgcgt aaactcgcgc aggcgctgga aagtatgcgc gtgaagctgg aagggaaaaa
     1981 ctatattgag cagtatgttt acgcattaac tcatgagcta aaaagcccac tggcggcgat
     2041 tcgtggagcg gcggaaattt tacgcgaagg tccgccgccg gaagtggtgg ctcgttttac
     2101 tgacaacatt ctgacgcaaa atgcgcgtat gcaggcattg gtagaaacgt tactacgcca
     2161 ggcaagactg gagaatcgtc aggaagtcgt tctaactgct gttgatgtgg cggcattatt
     2221 ccgccgcgtc agcgaagcgc gcaccgtgca gttggcagaa aaaaacatca ctctacatgt
     2281 tacgcccacc gaggttaacg ttgctgctga accggcgtta ctggagcagg ctctggggaa
     2341 tttactggat aacgccatcg attttacccc cgagagcggt tgcatcacgc taagcgccga
     2401 agtggagcag gaacacgtca cgcttaaggt gctggatacc ggtagtggta ttcctgacta
     2461 cgcgctttca cgtatttttg aacgctttta ctctttgcct cgtgcaaatg ggcaaaaaag
     2521 cagcggtctg gggttggcgt tcgtcagtga ggtcgcccgt ttgtttaacg gcgaagtcac
     2581 gctgcgcaat gtgcaggaag gtggcgtgct ggcttcgctt cgacttcacc gtcacttcac
     2641 atagcttcaa attcttccca catagccttc gtatcctgct gccattgcaa aggagaagac
     2701 tatgttgaaa tcccccctgt tctggaaaat gaccaccctg tttggtgcag tattgctgtt
     2761 gttgattccg ataatgctga ttcggcaggt gattgtcgaa cgtgctgatt accgtagcga
     2821 tgtggaagat gcgattcgcc aaagtaccag cgggccgcaa aaactcgttg ggccgctcat
     2881 cgctattcct gtgaccgagc tttatacggt gcaggaagag gataaaaccg tggagcggaa
     2941 acgaagtttt atccattttt ggttacctga gtcattgatg gttgatggca atcagaacgt
     3001 ggaagaacgc aagataggga tttataccgg tcaggtctgg cacagtgatt taacgctaaa
     3061 agccgatttc gatgtttcgc gtcttagcga actcaacgcg ccaaatatca ccttaggcaa
     3121 gccatttatt gtgattagcg tcggggatgc gcgtggtatt ggtgtggtga aagcacctga
     3181 agttaacgga acggcgctga ccattgaacc cggcaccggc ttagagcaag gcgggcaggg
     3241 cgtgcatatc cctttacctg aaggggactg gcggaagcag aacctgaagc tgaatatggc
     3301 cctgaattta agcggtaccg gcgatctttc tgtggtgcct ggcgggcgta atagcgaaat
     3361 gaccttaacc agcaactggc cgcatcccag ttttttaggt gattttctac cagccaaacg
     3421 ggaagttagc gagtcaggtt ttcaggcgca ctggcaaagc agctggtttg ctaataatct
     3481 cggtgagcgt tttgcttcag gcaatgatac cggctgggaa aacttcccgg cgtttagcgt
     3541 cgcagtaacg acgccagccg atcaatacca attaactgac cgggcgacta agtacgccat
     3601 tctgctgatt gcactgactt ttatagcgtt ctttgttttt gaaacgctca ccgcgcaacg
     3661 tttacaccca atgcaatatt tgctggtggg gctttcattg gtgatgtttt atttgctctt
     3721 gctggcgctt tctgaacata ccggttttac cgtggcatgg ataatcgcca gtctgattgg
     3781 ggcgataatg aacggtattt atttgcaggc ggtattgaaa ggttggtgca acagcatgtt
     3841 gtttaccctc gcgctgttgt tgctggatgg tgtgatgtgg ggactgctca actctgccga
     3901 tagcgcgctg ttgttgggaa ccagtgtgct ggtggtggcg ctggccggca tgatgtttgt
     3961 gacccgtaat atcgactggt atgcgttttc actgccgaaa atgaaagcca gtaaagaagt
     4021 tacaacggac gatgaattac gtatctggaa ataaggttga aaaataaaaa cggcgctaaa
     4081 aagcgccgtt tttttgacgg tggtaaagcc gattaatctt ccagatcacc gcagaagcga
     4141 taaccttcac cgtgaatggt ggcgatgatt tccggcgtat ccggcgtaga ttcgaaatgt
     4201 ttacgaatac ggcggatcgt cacgtctaca gtacggtcgt gcggtttcag ctcacggccg
     4261 gtcattttct tcagcagttc agcacgggac tgaattttgc ctgggttttc acagaagtga
     4321 agcatggcgc ggaactcgct gcgcggcagc ttgtactgct cgccatcagg gccgatcaac
     4381 gaacggctgt tgatgtccag ttcccaacca ttgaacttgt agctttcaac gctacgacgt
     4441 tcttcgctga cagtacccag attcatggta cgggacagca ggttgcgtgc acgaatagtc
     4501 agttcacgcg ggttgaacgg tttggtgatg tagtcatctg caccgatttc gaggccgaga
     4561 attttatcga cttcgttgtc acggccagtc aggaacatca acgcaacatt cgcctgctcg
     4621 cgcagttcac gcgctaacag aagaccgttc ttacccggca gattgatatc catgatcacc
     4681 aggttgatgt catattcaga gaggatctga tgcatttccg cgccatctgt cgcttcgaaa
     4741 acatcatagc cttccgcttc gaaaatactt ttcaacgtgt tgcgtgttac caactcgtct
     4801 tcaacgataa gaatgtgcgg ggtctgcatg tttgctacct aaattgccaa ctaaatcgaa
     4861 acaggaagta caaaagtccc tgacctgcct gatgcatgct gcaaattaac atgatcggcg
     4921 taacatgact aaagtacgta attgcgttct tgatgcactt tccatcaacg tcaacaacat
     4981 cattagcttg gtcgtgggta ctttccctca ggacccgaca gtgtcaaaaa cggctgtcat
     5041 cctaaccatt ttaacagcaa cataacaggc taagaggggc cggacaccca ataaaactac
     5101 gcttcgttga catatatcaa gttcaattgt agcacgttaa cagtttgatg aaatcatcgt
     5161 atctaaatgc tagctttcgt cacattattt taataatcca act
//
LOCUS       DAZGAP010000263         5199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGAP010000263 DAZGAP010000000
VERSION     DAZGAP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.264"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(68..1522)
                     /gene="amn"
                     /locus_tag="ACHLAM_004832"
     CDS             complement(68..1522)
                     /gene="amn"
                     /locus_tag="ACHLAM_004832"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO7856228.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1624..1950)
                     /locus_tag="ACHLAM_004833"
                     /pseudo
     CDS             complement(1624..1950)
                     /locus_tag="ACHLAM_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2224..2784)
                     /locus_tag="ACHLAM_004834"
     CDS             complement(2224..2784)
                     /locus_tag="ACHLAM_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HFO7856229.1"
                     /translation="MIEENGNFSFDAPGKESFKARLLSLIGTRSVRAAAKDWGVPVST
                     LNNYIHKGTEPSFKMACLISNKEHVSLNWLAFGSDENTPRTPCCSTVDAGNLDNANQT
                     ITAILSSLSTDEQLELARLLGRKGADFLTILLDDNIQELHALSGFRRELALRLKDMSD
                     SEAREIYEECEAKVNHLNVTQKKASA"
     gene            2970..3200
                     /locus_tag="ACHLAM_004835"
     CDS             2970..3200
                     /locus_tag="ACHLAM_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7856230.1"
                     /translation="MARNEVKPDWHPQRILGEVRAREGSMRALARKAGVGQNNLYNAV
                     YRHVPKYEGIIAECLGVPPEEIWPSRYIRKVA"
     gene            3203..>5199
                     /locus_tag="ACHLAM_004836"
     CDS             3203..>5199
                     /locus_tag="ACHLAM_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO7856231.1"
                     /translation="MFASVKELVGLPGMPGTEQGVRYSIRKYATSETSRRRQEGAKGF
                     EYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSSDVVDAVEAYRGSPQL
                     MVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERIV
                     AMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWLP
                     EFLAQYRQSNGRPMTEAYEDFVTEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVKQ
                     KGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKMKCRHPVHGRPFAPEVTFVID
                     GGTRFVVGWSMDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGIL
                     PRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLRS
                     AFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYSP
                     AEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHGR
                     KVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRAE
                     EKIRDAEDEVVNVLEHKQQEPWLENIYRPVGNTVAVQQPAV"
BASE COUNT         1430 a         1218 c         1322 g         1229 t
ORIGIN      
        1 caggctattt gccagatgtc ttacttaatc cttcacatct atttctgata agaaggttca
       61 taacttatta tcggaacggc ggctcattaa aggttcgtaa tttacgtgaa tgcagtcggt
      121 cgccttctgc gcgcagcaaa tcgatcgccc gaatgccaat ttgcaggtgt tcggaaatag
      181 cgccttcata aaaacggtta gcctggccgg gaagtttaat ctcaccatgt aacggtttat
      241 ctgaaacaca cagtagtgtc ccgtatggca cgcggaaacg atatccttgc gcggcaatgg
      301 ttgcactttc catatcaatt gctacggccc ggcttaagtt aaaacgaagt gcagaagctg
      361 agtaacgtaa ctcccagttc ctgtcatctg tggttaccac cgtgccagta cgtaaccgct
      421 gtttgacttc ttcaccaggc ctgccgctga ctaacttggt agcgtcataa agcgcacgtt
      481 gcacttccgc aatgctcgga ataggaatat cgggcgggag aactgcatca agaacgtggt
      541 catcgcgtaa ataagcgtgt gcaagtacat aatcgccaat ggcctgactt tcacgtaatc
      601 cgccacagtg accaatcatc aaccagacat ccgggcgtag cactgccaga tgatcgcaga
      661 tggttttagc atttgacggt cccacgccaa tattcaccaa agtaataccc tgaccatcgg
      721 cggtaattaa atgccatgct ggcatctggt gttttttcca tgcaaggtcg gaaatggctt
      781 cttctggcgc ttcggtttcg gcggtgatcc agttcccgcc agcacaagaa agggcaatgt
      841 agggactatc agggtcgagg atctggctgc atccccaacg aacgaattca tccacataac
      901 gtgtgtagtt ggtaaacaag acgaacggct gaaaatgttc aactggcgta ccggtataat
      961 ggcgcaaccg cgcgagggaa aaatcgacgc ggcgcgcatc aaaatgcgat agcggggaga
     1021 attccgttgg atgataaatg ccgtctgcag tttcatcgcc aatttgcgcc agttctgttg
     1081 tcgggaagta gcgagttaac ccagcgctca ttgagcgatc aagtgtcaat tcagagccat
     1141 cgatgacata aggataaggg atttcatgct gcgagggttg caccgagata tgcgcaccat
     1201 aatcctgata cagcaacgta agttgttcat tgagatacga acgaaagaga gtagggcgag
     1261 taatcgtggt ggtgtagctg cctgcgtggg taaaacgacc aaatgcgcgc gttttggggg
     1321 gattggttgt gctaccgtcc caggttacgg tcagtgatgg atagacaaaa agaccttgtt
     1381 tgcgggcgtt ttcatcaggt aattcgccac ttgtaatata tttgccaatg gcgttgcgta
     1441 aagcgactac agattgctca tacagcgcgt cgagtttatc cagtgcctga gctggggtca
     1501 gaccggagcc cttattattc atgtgtgtct cctgttccat acaattaccg cacatccgat
     1561 agtatgtcac aggaatgtga aacaaaagtg taaggcgaag atcggatgat cttcgccagg
     1621 ctattaagcg cgttgattgt ctttcatcaa caaagcggtc attgcggaaa tcaggcatcc
     1681 agccagcaaa taaatggcga cgctatgcca gttcccggca aagtaagtga tgagtgcagc
     1741 ggcaataaaa ggtgtaaatc caccgccaac cacactggca acctgataac cgactccagc
     1801 gccactatag cgataactgg caccaaacat ttcggtaaac atcggttgtt gtacacacac
     1861 caccatatca tgcgcaatgt ttgccagcat gatggagaag aaaactatcc agaaaataga
     1921 ttgtgcttca agcgccataa agaaaggaaa ttgtaaccag gtgaatcatt tgataccaac
     1981 gagggtttta gtgaaaggat gtgaaatcac ctgtaagata ctgaaataaa acaggtgata
     2041 ccaatgcgag cgcggaaagt ctatttgata ccgattgtgc aataattagt cgtttattct
     2101 aaaatcggca acacttttga tactacggat tgacgcttaa aatggtttta aaaaggctta
     2161 aaacgctttt ctccagattg tctttaaagg agtgtaccac agcacaaaat ttgcaccact
     2221 cccttacgca ctagcctttt tctgggttac atttaaatga ttaaccttag cttcacattc
     2281 ttcataaatc tctcttgctt cagaatctga catatccttt aaccttaaag caagttctcg
     2341 gcgaaaaccg ctaagtgcat gtaattcctg aatattatca tccaataaga tcgttagaaa
     2401 atcagccccc tttctgccaa gcaatcgggc taattctagt tgctcatcgg tgctgagaga
     2461 ggacagaatt gccgtaattg tttgatttgc attatctaaa ttacctgcat cgacagttga
     2521 acaacatgga gtgcgtgggg tgttttcatc agaaccaaaa gctaaccaat ttaaactcac
     2581 atgctcttta tttgatataa ggcaagccat tttaaaagag ggttctgtgc ctttgtgtat
     2641 gtagttattt aaggttgata caggaacccc ccaatccttt gctgctgccc ttacactccg
     2701 cgtcccgatc aggcttagta agcgggcttt aaaactttct tttcctggtg catcaaaaga
     2761 aaaatttcca ttctcttcaa tcattttttc ttttcctcat aacacattga aataaaaaga
     2821 gataaccaaa taaaacccac ttcagcccac ggagacaaaa gaaaaagcta tttttgcttt
     2881 acatgggcta catttggatc aatacttgta tcaaacttga taacaaaatt tagttatcaa
     2941 atttcgtaac tttttaggat agatgaatca tggcgagaaa tgaagttaag cctgattggc
     3001 atcctcaaag aatcttgggg gaagtcagag ccagagaagg aagcatgagg gcactagccc
     3061 gtaaagctgg cgtcggccag aacaatctgt ataacgcggt ataccgccat gtacccaagt
     3121 atgaggggat tattgcggaa tgcctgggtg taccacccga ggagatttgg cccagccgct
     3181 acattcgtaa ggtcgcgtaa ttatgtttgc ttcagttaaa gagctggttg gattaccggg
     3241 aatgccggga acagagcaag gcgtccgcta ttcaatcagg aagtatgcaa ccagcgaaac
     3301 cagcagacgt cgccaggaag gcgctaaagg ttttgaatac agcatcgact gtttacctga
     3361 agtgacgcag caggcattac gtgaacgtta tgccctgcaa ctgatgacgc aaaaagccga
     3421 tgaatcaccg gctccggtgg tgacaaaggc cagacgctca tctgacgtgg ttgatgcggt
     3481 ggaggcatat cgcggatcac cacaactgat ggtcgaacgc ctcaatgccc tgactgaaaa
     3541 ccagcgccag gtggctgatg cacgaatcgc gatcgtcagc gaagtgatga aagtcgcgca
     3601 acaacccggt ttcagctgtg cgaaagctat ccggtttatc gttgacaacc tggcacgttc
     3661 acagctggac gagcgcattg tggcaatggt tgagacggcg aacgccaaaa agggaaacag
     3721 ccgcgcgttg agtgaaatca cgctgaaacg ctggattgca gcctttaaca aggcgcagaa
     3781 cgccgccgaa cgcctgcttt tgctggcacc tggtaaacgc caggaaataa aagccgaaga
     3841 tattaactgg ctgcccgaat ttctggcgca gtatcgccag tcaaacggcc gaccaatgac
     3901 cgaggcttac gaggattttg ttactgaatg gcagcaccgg cacgctgatg agccttatat
     3961 gctcgatatc atgccgtctt acgacaccat tcgccgcgca atgaagaaac tgccggaagt
     4021 ggtgaaacaa aaaggccggg tgaccggcag tgaataccgc cagcttgagg gattcacgcg
     4081 ccgcgactgg tccagaatgc cggtgaatta tgtctggatt ggtgacggtc acggcatgaa
     4141 gatgaaatgc aggcatccgg ttcacgggcg gccatttgca ccggaagtga cctttgttat
     4201 cgacggtggc acgcgctttg tggtgggctg gagcatggac ctggctgaaa atgttttcgc
     4261 cgtagccggt gccatacagc acggtattcg ccatcacggc aaaccgtttc tgtattactc
     4321 ggataacggt tcgggggaaa ccgccgacat cctggataag gaggttgtgg ggatactgcc
     4381 gcgactgggg attaatcacc cgaccgggat tgccggtaat ccgcagggac ggggcattat
     4441 cgaacggctt aaccgcacat taccgatgcg catagcccgt aaataccgca cctatttcgg
     4501 gaaaggtgca gatcgcgaga cgttacgcaa aaccaaccgc gatttacgct cggcattcac
     4561 tgccctgcaa cagggcaagc ggctgaacgc ccggcagcag tcagcgatgc gtgatttgcc
     4621 gtcctggtct gaactgattg atgccattcg tgatggtgtt gagtggtaca acaaccggcc
     4681 gcacgatgaa ttacccatga agccgaacgg taagcattac agcccggcag agttcagaaa
     4741 aaaacgcctg gcagaagagg acacggaaat tgaatggctg tccgatgttg agcttcggga
     4801 tatgttccgg ccgatggtgg aacgccctgt aagacgctgt gaaatacgct ggctgaataa
     4861 tatttactac gcgcccgagc tgcgtgacga gcatggccgt aaggtgctta tcagctatga
     4921 cattcatgat gccgaacgaa ttaccgtacg tcgcctggat ggcagcgtga tttgcgaggc
     4981 ggtatgggac ggcaataaac gcgaagcctt cccggtcagc gcggaatact acaaacagca
     5041 gcaacgcctt aaaggcatgc gtaaacgcgc agaggaaaaa atccgtgatg ccgaggatga
     5101 ggtggtcaac gtgctggagc acaagcagca ggagccatgg ctggaaaaca tctaccgccc
     5161 ggtgggtaat acggtggccg ttcagcaacc tgccgttga
//
LOCUS       DAZGAP010000264         5195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGAP010000264 DAZGAP010000000
VERSION     DAZGAP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.265"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            363..1988
                     /locus_tag="ACHLAM_004837"
     CDS             363..1988
                     /locus_tag="ACHLAM_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856232.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDAHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            1985..3253
                     /locus_tag="ACHLAM_004838"
     CDS             1985..3253
                     /locus_tag="ACHLAM_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7856233.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            3268..3546
                     /locus_tag="ACHLAM_004839"
     CDS             3268..3546
                     /locus_tag="ACHLAM_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856234.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            3552..4169
                     /locus_tag="ACHLAM_004840"
     CDS             3552..4169
                     /locus_tag="ACHLAM_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856235.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRETNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            4260..4994
                     /locus_tag="ACHLAM_004841"
     CDS             4260..4994
                     /locus_tag="ACHLAM_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7856236.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
BASE COUNT         1367 a         1064 c         1410 g         1354 t
ORIGIN      
        1 agagtttttg gtgtttccat accagccaca gttgatgtgt gttgtggcac ctggcagtac
       61 aattaaggga acaggggtaa taaaaaacgg gggcatgacc aacccccggt acaaggaagg
      121 agtaactaca atggaaaaat caatgaagac attcagtgct atgttatatg gcgaataaag
      181 actgccacag ggaagtttgc agaaacagtg atgccgatcg tgtttctgac gctcttctct
      241 gataagagta atttttcaga tttctggaaa tctgtattct gcacaggcgc aattgtatgc
      301 cgcctttaaa acttcaattc agtgactcac ctgccattca aattttcgga taccagacaa
      361 ccatgcctta tatcgatata acaactatgc gcgggatgat gccaggcgtt attgcatcta
      421 tgctgccaga tcattctgct gtactggcag aaaactgtca ttttcgctat ggagtgatca
      481 cgcctgaaca ccagatgtca gaggctgaga aaacatttgc gattaagccg aaaaccattt
      541 ttcattaccg tgacgatttc tggtttgcat ggacggatgt ggtggatgtg atccgcagtc
      601 cggtcgctca ggacgcccac gggcgtattt actacactga cgggcgtttt cctaaagtga
      661 cggatgcgac cattgccaca aaaggggacg ggaatcaccc gacatcatcg tatcgtctgg
      721 ggattcccgc gccgacaaca gcacctgtct gtactgttca gcagggcggt gatgtttctg
      781 acgataaccc gaatgatgac gaaacccggt tttatacgga aacctttgtc tcagattatg
      841 gtgaagaagg tccgccaggt ccggcgtctc tggaggtaac actccgtact ccggggactg
      901 cggtacagct gacgctgtct ccggtgccat tgcagaatgc cagtattaaa cgccgccgga
      961 tttatcgctc tgcatcaggt ggaggagaag cggatttttt acttgtggct gaactggatg
     1021 catccgtgct cagttacacg gacaaaatac cggggaaaaa ccttggacct tctctggcta
     1081 catgggatta cctgccgccg ccagagaata tgacaggcct ttgcctgatg gctaatggta
     1141 ttgccgccgg gtttgccggt aatgaagtga tgttttcgga agcgtatctg ccgtatgcat
     1201 ggccggaagt gaatcgtcac acgacggcag aagatattgt ggctatctgt ccgctgggaa
     1261 cgtcactggt ggtggcgaca aagggggagc cttatctgtt cagtggtgta tcgccttcca
     1321 caatttctgg ctccagaatt ccttccatgc aggcatgcct gagccgaaga agtatggtgg
     1381 cgatggaggg attcgtactc tatgccggga caaacggtct ggtatctgtt gatgtaaacg
     1441 gtaatacagc actggcaacg gaaaagatta tttcacctga acagtggcag agtcagttta
     1501 acccggcgtc cattgtggct tattcctggc gtggtgaata cattgcctgt tacacgaaac
     1561 cggatggtaa gcaggatgtg tttgtattca gtccggtgaa catggatatc cgttatctca
     1621 gtacaccgtt tgactgcgca tgggttgatc tcgcgaaaga tatgatgcgt gtggtgacag
     1681 gagacaaaat gtcagtgctt gccgggggct ctctgccctc cacgataagg tggcattcaa
     1741 aaattttttc attacctgaa agaacctctt tttcctgtat cagggtgaaa tctccggcgc
     1801 ctgagcgggt ggggatcacc attatggctg atgatgttcc tgtgattcat tttgcgccgg
     1861 gtacgtttaa gggaagtgtg gtgagacttc cggcagcaac cgggcaaaac tggcaggtga
     1921 tggtatccgg attcgggcag gtggaacgaa taaccctgag tacatcgatg tcggagatgc
     1981 cggtatgacc agaaaaccgt ggcgtgcggg gaaggattta tccacagttg tggagaacat
     2041 ggaaattggc accgggcagc gtggtgacgg acgccacgca tttgtgaccc gtgaggaact
     2101 ggttggtctt aaactcgccc ggcgtcgaac atcgggtggt gcctcatatg cactgaatcc
     2161 gggtattgag attgacagta ctttaatgac tgttgatttt cccacaaaac cgctgaattt
     2221 taaggcgaca ggaggatttg gctcggttct tcttgaatgg gatatgccta attatcgcgg
     2281 acattcactg actgaaatct ggcggggtac ggaggatgac cttgctgatg cagtgctggt
     2341 tgccacgacg ccggggcagg tttacggcga tccggttgac cctggctggt cgggatttta
     2401 ctggatacgt tttgttaacg cggcaggagt gaaaggtcca tggaatgctg aaaaaggcac
     2461 tcaggcacaa acacagatcg gcgtgaaggc catcattgac cagatccgcg atgaggctgc
     2521 aaagtcgccg gttgtgtccg agctgcgtaa agaaataaaa aacgcgcagg ggcaggctgt
     2581 aaaggatgct gcaattaaga caaccgaagt tgtggggact ctcagggaag aaacgacaag
     2641 aacgattggt ggtattgaaa cccgcattag cacactggat tcgtcaacca gtgaatcgct
     2701 taatgaggtc gacaagcgca tcactaaact ggataaagaa ggcggtgagg cgtttctggc
     2761 aatgtggtca aaaaaagcgg gagttgatgg tatcactgcg gggatcggga ttgtcgccgg
     2821 aaaagacagt gaaggcaggc ctgtaagtca ggttgcaatt tctgcgtcgc agttgtttgt
     2881 ctttgacccg aacaacccgg ataacaccgc ctatccgttt gcggtatcag gtggcaaggt
     2941 tgtgatcccg aaagcgatga tttatgacgc ggtgattgaa acactggtgt cgcggaaggt
     3001 tgtggcggat gaggtaaaag ccggggtaag tatcacttcg ccagttatcc ggagtgccgt
     3061 tattcagaac ggaaactttc aggttgattc tcagggtaac ctgaatattg gaggcctttt
     3121 cagtgttacg tcacaagggc aactgacaat tcgttactct aatcagaatg taggactggt
     3181 gatccgcaat gataaaattg aggtttatga tcagaatgga cgactggctg ttcgcatagg
     3241 cagattacgc tgatcaggag gtgagtattg gaatacggtt ttgccattta taacagaaat
     3301 aacgttaatg ttacgggcgt gctgactccg gtatttttcc tggacagatt tacagcggag
     3361 tctggctcaa agacgtacac gaataaaccc gacgggaaat cattgcaggc tgtatgttgt
     3421 ttatttccct ggaataatgt atttgcggat cggaaagtac cgaagataac cattaatggc
     3481 aatacggtga cgtggtcgaa tcttgagcag ggtatgggat cttatattta tacattctgg
     3541 ggataagtgt catgtatggt ttgagcatta tgaagccgga tggcagcgta tggataagtc
     3601 caggttttac gccgcagtgt ctgatcaaca aaggcaccat accggcgact gaaaagtctt
     3661 tttttaaaac atcaatcccg tcaggcaaaa gttgtttttt ctttatcaga acagagaaga
     3721 aggccgatgt catgtacacg catgaacaga ttgatggata tcatgcacta aggcttcatg
     3781 taattgtcag ggaaacgaac cctggtgtta cgacggttta tgctttcgcg aatatggtta
     3841 ctccaccttc tgagtatggt atcgccatgt ataacccgga cggtgagatg atttatcatg
     3901 gcgaaatgat gctgcttgac gcgaagttaa tacctgttga tatcaaattt gaaaaggacc
     3961 ttggatatcc atgcgcaatc atgccagcac tggtcgggta ttataactgg aaaagaactc
     4021 cttatgatcg accgatttat accacatcca ctggtgctac aggaaataaa atatattcct
     4081 gtgagcatta ttccggtggt gcaacatggg atattcgaaa gccgtatata gataaggtcc
     4141 tggttattaa tacatcagta tatgattagt tgaagcgagt ctttaatatt catttaaaat
     4201 gtctaaaaag atgtattatt aaaaagttta gcgtgttatc tgaatacagg atatcttaaa
     4261 tgaagagtat agcaacactg gttgtgtgtg caatctccgg gattgcctgt gtaaatttat
     4321 ctgcacatgc agcagaagga aaccatacaa tttctctggg gtatgcgcac tttcagtttc
     4381 cgggactgaa ggattttgta aaggatgcga ctgctcataa cagggagact ttcagtcatt
     4441 tcgtcaacag aaactacttt tcttcattgg gcgaatatac agatggtcgg gtcagtggat
     4501 atgaaggcaa ggataaaaat ccacagggca ttaatatcag gtatcgctac gagataacgg
     4561 atgattttgg cgttatcacc tcttttacat ggacgcgttc tctcactaac tcacagacat
     4621 ttattgatgt gcagtcagcc gatcatacca ggaagattaa gaatccggca gcttctgcca
     4681 gaacggatat cagggcgaat tactggagtc tgttagcggg gccttcatgg cgggttaatc
     4741 agtatatgag tttatatgcg atggcaggga tgggcgttgc taaagttagc gctgacctga
     4801 aaattaagga caatattaac agtagtggcg gattttctga aagcaacagc acgaaaaaaa
     4861 cctcccttgc gtgggctgca ggtgcacagt ttaacctgaa tgagagtgtt acactggatg
     4921 tggcttacga aggttccggc tctggcgact ggcgcacgag tggcgttact gctggcattg
     4981 gcctgaaatt ctgacctgta tccggtaacc gtttactacc cgctgtgatg gcgggttttt
     5041 tattgcccgt acagggcaaa aaccgtaaat tatgcgtggg tgcctttcgg ctgatggctg
     5101 gagggagaac ctgaaggcct gatgtggaaa ggccccgagt caacttaacg ttaacccgag
     5161 gccctaatca tcatacctta agcaagtaga aggtt
//
LOCUS       DAZGAP010000265         5168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGAP010000265 DAZGAP010000000
VERSION     DAZGAP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.266"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..529)
                     /locus_tag="ACHLAM_004842"
     CDS             complement(<1..529)
                     /locus_tag="ACHLAM_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7856237.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQE"
     gene            1007..1795
                     /gene="fabI"
                     /locus_tag="ACHLAM_004843"
     CDS             1007..1795
                     /gene="fabI"
                     /locus_tag="ACHLAM_004843"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO7856238.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1939..3066
                     /locus_tag="ACHLAM_004844"
     CDS             1939..3066
                     /locus_tag="ACHLAM_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO7856239.1"
                     /translation="MEKRHITGKSYWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARSIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMNSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            3134..5068
                     /gene="rnb"
                     /locus_tag="ACHLAM_004845"
     CDS             3134..5068
                     /gene="rnb"
                     /locus_tag="ACHLAM_004845"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO7856240.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT         1237 a         1367 c         1264 g         1300 t
ORIGIN      
        1 tttcctgcag atgttgtgga tcgctttttt gcggtgttaa acgacgataa accgtacctt
       61 ccatcatggt agcaaacact tcaacgcagc aacgaatgtg ctcgtcgcta agatggggat
      121 gcattctttt catatgtgca caaccgttag cgaacattct ggcgtcagct tcttcaagca
      181 ttgccataac ccgcgggttg cgtgtggctt ctgcagccac ttccagcatc agcgcgtcgt
      241 cgtcctcatt taaggattta cgcaaggcaa ggacttccgg gaagtgatcg gtacgcgcgt
      301 caatgtccat ttgggcaata cgaaagtcga tgatacggcg aaccatctct tcaataatgg
      361 cgtctttgtt ggcgaagtaa cgatatatct gacccacgct aagctgagct tcgctggcaa
      421 tttgcgacat actggcggca tgaaatccgc agcgacgaaa acaccgacgg gcggcgttaa
      481 tgatttcatc ctgacgctgt ttgtggcgta tctccagctt gctggtcatt tctgtctcct
      541 ggcctatcac ttgatgagaa tgatcgttct catttttgct aattatacgt ttctccaaag
      601 taatggaaag tatttttatc ttattcatga tgcatattct gataatgaag agattgtgga
      661 gattctgtga agatagttga caatattaag ttggggtcaa ttcatcagaa agacagcgac
      721 ttgttgtcgc cagttctgtt ttcatcatca aatttatcgg agctaatacg aatagtttca
      781 tagtcagatt ctataagata tttccccaac ctcccccttg caggcgtcag gagcagataa
      841 cgcaaaatgc tctgtaccgc agcttctctc cgctacagaa agcgcaacta tagccaccca
      901 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
      961 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
     1021 cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct acggtatcgc
     1081 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
     1141 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt
     1201 tgccgaagat accagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
     1261 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
     1321 taacgccgtt actcgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
     1381 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
     1441 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
     1501 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
     1561 cgccatctct gcgggtccga tccgtactct ggcggcttcc ggtatcaaag acttccgcaa
     1621 aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt
     1681 gggtaactcc gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtggt
     1741 ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga aataatcgtt
     1801 ctgttggtca agacgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
     1861 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
     1921 tacgaccaag gagcgcccat ggaaaaacgc cacattaccg gcaaaagcta ctggtatcat
     1981 gaaacgcaat ccagtactgc ggagtatgac gttctgcctc tggtcccgga agccgcaaag
     2041 gtcagcgatc cctttctgct cgacgtgatc cttgatgaag aaacgctggc ccccttcctt
     2101 tcatggctgg tccctgcgcg cgttcttgca gtggaattgt tccctgacca gcttaccgtg
     2161 acccgttcac agaccttcac cgcttatgaa cgcttgtcga cggccctgac ggttgctcag
     2221 gtttgcggcg tccaacggtt atgtaactac tattcggcgc gacttacgcc gctccccggg
     2281 cctgattcct ccagggaaag taatcatcgg ttagcacaaa tcacgcaata tgcccgccaa
     2341 ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggc
     2401 ctaactgcct gggactgtgt aatcattaac caaatcattg gttttattgg ctttcaggcg
     2461 cggtcaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt acccggtctg
     2521 gagatacaaa actatgccga cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc
     2581 agctatgagg tggaaaaact ccctgaagag cacacaaaaa gttcaactgc agaactttgc
     2641 caactggccg aaacactctc tctccaccct atttcacttt cccttctcga aaagttgcta
     2701 aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt atgcgcgcgg
     2761 ataaatggca gtcctgcttg ttttgccacc tgtatgaatt catcaaatga atataaaaaa
     2821 atcagcgccc ttctgcgcaa gggcgaaaat gaaattaacc aatgggctga ccgtcattct
     2881 gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
     2941 gccgcccagt tcagcccatt actcgaacac gaaaaatcat caacgcagat tattaatctg
     3001 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
     3061 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
     3121 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
     3181 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct ttggcttcct
     3241 ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
     3301 tggcgaccga attatcgcgg ttatccacag tgaaaaagaa cgtgaatccg cagagccaga
     3361 agaactggtt gaaccgtttc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
     3421 tctggccatc gttcctgatc atccactctt aaaagacgcc attccttgcc gcgcagcccg
     3481 tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc
     3541 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggtgacga
     3601 ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggagaaag aagcaccaga
     3661 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga ccgcgctgga
     3721 ttttgtcacc atcgacagtg ccagcacaga agatatggat gacgcccttt tcgctaaggc
     3781 gttgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
     3841 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
     3901 cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa
     3961 tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
     4021 taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt atgacctggt
     4081 ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa ttgctgaaca
     4141 agtccgtttg ttagcgcaaa tttgccaacg ccgcggcgag tggcgtcata accacgcact
     4201 ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
     4261 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc
     4321 taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct ataacgtgca
     4381 tatgggcttt gatccggcga atgccgacgc gttggcagcg ttgctgaaaa cgcacggtct
     4441 gcatgtcgat gctgaagaag tgctcacgct ggacggtttc tgcaaactgc gtcgtgaact
     4501 ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
     4561 aattagcact gaacccggtc ctcactttgg cctcggtctg gaggcatacg ccacctggac
     4621 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
     4681 aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg agcgtcgccg
     4741 tctcaaccga atggcagaac gtgatgttgg cgactggtta tacgcacgct tcctgaaaga
     4801 caaagccggg accgacaccc gtttcgcggc ggaaattgtc gatatcagcc gtggcggcat
     4861 gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt tcttacacgc
     4921 tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta aaggtgaaac
     4981 ggcttacaaa gtgactgacg ttattgacgt caccattgcc gaagtccgca tggaaacccg
     5041 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct tatgaatggg
     5101 ccgtttattt ccccgctcta ccttcatcat atcccggcag ttttttaact gactttcgtt
     5161 tgaaaact
//
LOCUS       DAZGAP010000266         5129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGAP010000266 DAZGAP010000000
VERSION     DAZGAP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.267"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..840
                     /locus_tag="ACHLAM_004846"
     CDS             <1..840
                     /locus_tag="ACHLAM_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO7856241.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALT
                     SGEDVQLAAGQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQ
                     AQNGSMRLFAEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKV
                     KRTIAAGAASQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGN
                     LDGAGYLHLEDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSE
                     GVE"
     gene            837..2381
                     /locus_tag="ACHLAM_004847"
     CDS             837..2381
                     /locus_tag="ACHLAM_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856242.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            2378..3514
                     /locus_tag="ACHLAM_004848"
     CDS             2378..3514
                     /locus_tag="ACHLAM_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFO7856243.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHGDYLMYPPHNNVCNFF
                     DAAYLLELDNYKYNFNKLAYPVFPENLPPVPERSDIIIKTDDPVPCDGIWEPVKIHYD
                     HKLLIIKDGISGFNNLGAYNYFIRGMNAPRQVYYDVLIESDTEMVVPDDPIRYRDVHW
                     RLVWEDTRYSDGIIPDESEYFLDDAPGKRITCPSGELCPHSGHWATIAGGHQQFIDVQ
                     AGQLMPEATKYQSNMYADEIHLPAIWNLLRRDDEGSVYVKTGES"
     gene            3592..4014
                     /locus_tag="ACHLAM_004849"
     CDS             3592..4014
                     /locus_tag="ACHLAM_004849"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO7856244.1"
                     /translation="MDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVMEMNSIT
                     DFRERLCELEAALPSVSWMISSSSLTELAAHFRRNMNTELPDGRIALLRFHDPRVQKR
                     LGEMLNDQQHRELTGLMQEWLTIVDGKAWSFKQREFIC"
     gene            4008..4352
                     /locus_tag="ACHLAM_004850"
     CDS             4008..4352
                     /locus_tag="ACHLAM_004850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856245.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVRDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            4352..5116
                     /locus_tag="ACHLAM_004851"
     CDS             4352..5116
                     /locus_tag="ACHLAM_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFO7856246.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQQIQ
                     EFKYCGVSFDGWKDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAVAT
                     VNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQP"
BASE COUNT         1397 a         1007 c         1503 g         1222 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa accgctcaat gaagccgtgc ttttctcggc gccggaaggc
      121 attgccctga ccagcggtga agatgtgcag ctggctgctg gtcagaacct ggctgtcaac
      181 gccggaggtg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagagg
      241 ctggggcttt ttgccaggag cggacaactg agcgtcaaat ccggggaggg gccagtagag
      301 gtacaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac gctgagttcg
      361 gcaagtgata tctcctttgc cgggaaaaaa cgtatcacgc tgattggcgg ggggagttac
      421 ctgcggctgg aggcggggaa aatagagtac ggtacgacgg tgtcgtatct gcgcaaagtt
      481 aaacggacta tagcggcggg ggctgcgtca cagcagccgg aaatgccttg gcttgccgac
      541 ccggttaaac cctctgccag tgcgctgatg cagtatctgt atcacgacga tgtcccggta
      601 gtgggcgcgc ctttcaaagc gactcttgct gatggcactg tacgggaagg taaccttgac
      661 ggtgcgggat atctgcatct ggaagacgtc ccgccaggga gagtgctggt ggagctcggg
      721 ccggatacca gagattattt acgtaaaaat aagaccgata atcggttgtt tgttgggaca
      781 tctctcagcg aggctgacat tgactcactg atagcgaagt acagcgaagg tgtggaatga
      841 gtggagtaaa cgttgcctgg tttaaggaaa ccagcctgga aaccggacag tggatctggg
      901 ggacgcttca gggggcattt aatgaaaaac agaccattgg gcagatcatc gttgatgcgg
      961 tgatcggcat gatcccgctg gtcggcgatg taacggcagt gcgagatctg atagcggttt
     1021 cgataggact ggcaaaagaa ccgaagaaac gggaaagtgt gactgaatgg gtgtttctgg
     1081 tgatcctgct gtttgcgcta atcccggtaa ttggtggcgt gataaaaggt gtcggacgtc
     1141 tggccctgcg tgtgacaaca aaagtggcag agaatgtgga aacccttggc gctgtgatta
     1201 agtttctgaa ccgtatcggc gaaggtgatg cggtgaagtg gctgaataag ctgaatgtcg
     1261 ttgagtatca ggcacagttg attgaaaaga tgggggattt ttgcaacacg atgattaccg
     1321 ccatcaataa aatcctgaaa gcccgggtcg ggcggatgtt gccggagaaa tggcaggcgg
     1381 atttactctt ggttcgggat ggttttgcgg cactgcaacg gctggcgtcg agaatgatcc
     1441 cgcaggcgtt gaaagagctg gatgcgaaac tgcgggtgtt gcaggcgctg gtttataaag
     1501 gtgaaataca tacaatcacg acggggatgg gacgtgctga agtgcgccgt gaggctgaag
     1561 catatcttat tgagcgtccg ctgtgtgaag cgccgaagca ggggacgcgg tatccgagtg
     1621 ttcatgcgga ggtaattcac gaagacagat tgcggcttaa atattccagg gagggatatc
     1681 cagatttgtt gggatataaa ggaaaaagtc cggcatttgg tgatgagcaa gtatttacaa
     1741 atattgcctc ttttagcggc gagattaccg caaaaagtgc aagtgagatg ggcggtagta
     1801 cgatttttcg cgcttttggt aatccaacag aattagcacc caacggttct tgggcggctg
     1861 gcggattctg ggggtatcaa aaagtgccgg gcagtgctga agaatggcgt cagctttcag
     1921 ctgtactgga tgagtggaat ggtaacggtt ttctggtggt gctgcatttg ccagaagacc
     1981 tggcaactcg tatgccgggt agcaatgctt ggcacgggaa aattgctgaa cagttcggac
     2041 agaagagacc tgaccagtat ctgcaaggtg gtggcgaaca ggtaattatt aatctggatg
     2101 aaacgttgaa aacagagttg aaccgcatcg gggagcaggt aaaagccagc ggtcaggctg
     2161 tcagtattga aattaatggc gtaaatgccg agttttatcc gacgggctgg aaagaggtgg
     2221 aaggcgtgca tggttacagt aagccggaag ggatagaacc gccgtatgtg acgtataccc
     2281 gtgaactgca tgacgatgaa ctgcgcagca aagtgacaga gcgaacgata cttaatgcgg
     2341 cccgcattga gtaccagacc acgacaggaa gcgaacaatg attaccattg aaaaaacaaa
     2401 atacccgatg agcgatgagc aggtacgtcg caaattattc tgggtgttac agcggctgag
     2461 cagctatagc ctttggcagc gcaaacggga tgcctgggcc tattttaccc agcagtatga
     2521 acaggcgctg aaaaactggc cggaagagat aacgaagggg tttgatcccg aggcgataat
     2581 ctgggcatat gatgctttgc gtctttacga tgaagggcta tcagaattgg cagtgggaaa
     2641 tcggcaggta tggcagcgtt taacgggaga atttcatcaa cttgctcgcc cagtggattt
     2701 aattgaatct tatttttatc tcccctgcca tgaacgaggt gtacagcaag aaccataccc
     2761 ggtagaagta gaaaagctta ataagctgaa gattgcagca aaaatacatg gtgactatct
     2821 aatgtatcca ccacataaca atgtatgcaa ctttttcgat gctgcatatt tattagaatt
     2881 agataattat aaatataatt ttaataagtt agcgtatccg gttttcccag aaaatctccc
     2941 ccctgttcca gaacgcagcg atattattat taaaaccgat gacccggttc cctgtgacgg
     3001 catctgggaa ccggtaaaga ttcattacga ccataaacta ttgattatta aagatggtat
     3061 cagcggattt aataacctcg gtgcttataa ttattttatt cgtggaatga atgctcctcg
     3121 ccaggtgtat tacgacgtat taattgaaag tgacacagaa atggtcgtac ccgatgaccc
     3181 aatccgttac cgggacgttc actggcgtct ggtctgggaa gacacgcgct acagtgacgg
     3241 aattattccc gatgagtcag aatactttct ggatgatgca ccaggcaaac gcattacctg
     3301 cccgagtggg gagctttgcc cgcattcagg tcattgggcg accatagccg gaggacatca
     3361 gcagtttatt gatgttcagg ccgggcagtt gatgccagaa gcaactaaat atcagagcaa
     3421 tatgtatgca gatgaaatcc atcttcctgc gatatggaac cttttacgcc gggatgatga
     3481 ggggagtgtt tatgtaaaaa caggggaaag ttaaataact gatcaacgct ttttgacgga
     3541 cagatctgtt accacctgat ttgctcaaca aaagtgacat tatttgctct ggtggatggt
     3601 cagcggtatt tcggtgagag cctgtctgtt caacaacctg ccgccgtgcc cctgttcgat
     3661 acctggcctg attcgcgtat tgcatttgcg gggccatggg ttatggagat gaacagtata
     3721 acggatttca gagaaagact gtgtgagctt gaagctgcgc ttccgtcagt ttcctggatg
     3781 atatcttctt cctcgcttac tgaactagct gctcatttca gaaggaatat gaatacagaa
     3841 ctaccggatg gacggattgc gttgctgcgt ttccatgatc cgcgcgttca gaagcgcctg
     3901 ggagaaatgc tgaacgacca acaacatcgg gagctgacgg ggttaatgca ggagtggctg
     3961 acaatagtgg acggaaaagc ctggtcattt aaacaaaggg agttcatatg ctgaagttgt
     4021 ctgaagagca gtgtgttggt atggacaaaa gggaagatga tgattttgtt cgcgacaccg
     4081 cgctaagact gaagaagaaa tttccgggaa ttactgaatc agatgacacg ttccatgttc
     4141 gcctgagaaa agcgctggat tatgccaata cccttccgct taaagagaaa aacgtgaagc
     4201 gggacttctt gtttctggag gccttctggc ctggttttta tctgaaaccc gaagtggata
     4261 aatggttgag aacgccgaat gcttattcaa ctgaccagcg ggttgaagat tataagcatg
     4321 tgatgataaa cagagaaagg agggagctct gatggttatg gtcctgacag gggctggtat
     4381 ggggagcgca gctaccggag gtgttggtat ggctggtgcc tcaaccggaa cagcagtatt
     4441 tcccggagca ggaagccgcg atggagactg ggaagaagat aatatatctt atcctggctc
     4501 aggcagtggt tcgcgtcagg gtaacggagg gttttattat caggggaaac agtatgatcc
     4561 aattctgaat gagccgatgg ttgtcagcgc agagacgcat ggtgatttct gggaagatga
     4621 tatggatcat tcagaaacag taaactatga aggtattgat agcaaagccg aagtacaggc
     4681 gaagacggat gagaaaacgt gtgagaactg cccaccggaa gggaaggtaa tgccgatgat
     4741 aaggcgttgc tcgcgctgga gtatggtaac aatcagctac cagacaagaa tatgtggcac
     4801 gttttataat ccggagacgc aacagataca ggaatttaag tactgtggcg tatcgtttga
     4861 tggctggaaa gataagctgt gtcagttctg ggaagctaaa gcgcggtatg accaattttt
     4921 cagggctgat ggttcaaaga aaccctggtg gacaggtaat ctaagcgcaa ttaatcaagc
     4981 atcccgacat caggcggttg caacagtcaa tcagccattg aaagttgtct ggatatttat
     5041 gcaacctgta agttgtgagt atttcaggaa aatgtttaaa gattacaagg atattattac
     5101 gaggtggcaa ccgtgattga attatttaa
//
LOCUS       DAZGAP010000267         5123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGAP010000267 DAZGAP010000000
VERSION     DAZGAP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.268"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..1802
                     /locus_tag="ACHLAM_004852"
     CDS             6..1802
                     /locus_tag="ACHLAM_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO7856247.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1820..2488
                     /locus_tag="ACHLAM_004853"
     CDS             1820..2488
                     /locus_tag="ACHLAM_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO7856248.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSLA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2503..3174
                     /gene="dsbI"
                     /locus_tag="ACHLAM_004854"
     CDS             2503..3174
                     /gene="dsbI"
                     /locus_tag="ACHLAM_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFO7856249.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIVRG"
     gene            complement(3220..4008)
                     /gene="ygiD"
                     /locus_tag="ACHLAM_004855"
     CDS             complement(3220..4008)
                     /gene="ygiD"
                     /locus_tag="ACHLAM_004855"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO7856250.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4151..4924
                     /gene="zupT"
                     /locus_tag="ACHLAM_004856"
     CDS             4151..4924
                     /gene="zupT"
                     /locus_tag="ACHLAM_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO7856251.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(4982..5122)
                     /locus_tag="ACHLAM_004857"
     CDS             complement(4982..5122)
                     /locus_tag="ACHLAM_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856252.1"
                     /translation="MALVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNSTREETAEK
                     VE"
BASE COUNT         1304 a         1276 c         1265 g         1278 t
ORIGIN      
        1 tatccatgtt tgataaatat agaaaaacac tcgtagccgg aactgtggcg ataaccctgg
       61 gtttgtcagc atcgggggtg atggctgcgg gttttaaacc agcgccgcct gccgggcaac
      121 tgggtgcggt cattgtcgat ccctacggca atgcaccact gaccgctttg gttgacttag
      181 atagccatgt tatttctgac gtcaaagtta ccgtccatgg gaagggcgaa aaaggtgtag
      241 aaatcagcta tcccgtgggt caggaatcac taaaaactta cgatggtgta ccgatttttg
      301 gtctttatca gaaatttgct aacaaagtga ccgttgagtg gaaagaaaac ggcaaggtca
      361 tgaaagatga ttatgtggtg cacacttcgg ccatcgtcaa taattacatg gataaccgct
      421 ctatatccga tttacaacag accaaagtta ttaaagtcgc accgggtttt gaagatcgcc
      481 tctatctggt taatacccac acctttaccg cccaaggttc cgatctccac tggcatggtg
      541 agaaagataa aaatgccggt atccttgatg cgggtccggc aaccggcgca ctaccttttg
      601 atatcgcgcc ctttactttc atcgtcgata ccgaaggtga ataccgctgg tggctggatc
      661 aagacacttt ctacgatggt cgtgaccgcg atatcaacaa gcgtggttat ctgatgggta
      721 tccgcgaaac gccacgcggc acctttaccg ctgtacaagg tcagcactgg tacgagttcg
      781 acatgatggg gcaggtgctc gaagatcaca aactaccgcg cggatttgct gacgccactc
      841 atgagtccat tgagacgcca aatggcacgg tactgttgcg cgtaggtaag agtaactatc
      901 gtcgcgatga cggcgtacac gtcaccacca ttcgtgacca tatcctcgaa gttgataaat
      961 ctggtcgcgt tgtcgatgta tgggatctga ccaagatcct cgatccgaaa cgcgatgcac
     1021 tgctcggcgc gctggatgca ggtgcagttt gcgttaacgt tgaccttgcc catgcaggac
     1081 aacaggcaaa actggaacca gatacaccgt tcggcgacgc gctgggtgta gggccaggcc
     1141 gcaactgggc gcacgtgaac tctatcgcct atgacgcaaa agatgactct atcatcctct
     1201 cctctcgcca ccagggtgtt gtgaaaattg gtcgtgataa gcaagtgaaa tggatccttg
     1261 cgccctctaa aggttgggaa aaaccgctgg ccagcaagct gctgaaaccg gttgatgcta
     1321 acggtaagcc aattacctgt aacgaaaatg gcctgtgcga aaactcagac ttcgacttta
     1381 cctacaccca gcataccgcc tggatttcca gcaaaggaac gctcaccatt tttgataatg
     1441 gcgatgggcg tcatctggaa caacctgcct taccaaccat gaaatactcc cgctttgtgg
     1501 aatataagat tgatgagaag aaaggcaccg ttcagcaagt gtgggaatac ggtaaagaac
     1561 gtggctacga tttctatagc ccgattacct ccatcattga atatcaagcc gaccgtaaca
     1621 ccatgtttgg cttcggtggt tctattcatt tgttcgatgt cgggcagcca accgtcggta
     1681 agttgaacga aatcgattac aaaaccaaag aagtgaaagt ggaaatcgac gtgctgtcag
     1741 ataaacccaa tcagactcac tatcgtgcac tgttagtccg tccacaacag atgttcaaat
     1801 aattaagaag ggaataaaaa tgtcgaaatt agggatctca tcactattta aaaccatact
     1861 gcttaccgca gctttggctg tttcatttac cgcttctgca tttaccgaag gcacggatta
     1921 catggttctg gaaaaaccta ttccgaatgc cgacaaaacg ctgattaagg tattcagcta
     1981 cgcctgcccg ttctgttaca agtacgacaa agcggttacc ggtccggtat cggaaaaagt
     2041 gaaagatatc gttgctttca cgccgttcca tctggaaacc aaaggtgaat acggcaaaca
     2101 agcaagcgaa gtctttgcgg tcttgatcaa taaagataaa gcagcaggca tttccttatt
     2161 tgatgctaac tctcagttta agaaagccaa gtttgcctac tacgcggctt atcacgacaa
     2221 aaaagaacgc tggtctgacg gtaaagatcc ggctgctttt atcaaaaccg gtctggatgc
     2281 cgcaggtatg agcttggcgg attttgaagc tgcactgaaa gaaccagcgg ttcaagaaac
     2341 actggaaaaa tggaaagcct cttatgatgt cgccaaaatc cagggggttc cagcctatgt
     2401 cgttaatggt aagtacttga tctacaccaa gagcatcaaa tccatcgacg cgatggcaga
     2461 ccttatccgt gagttagcaa gcaaataaca gggaaacagg ctatgggtat taagggaatg
     2521 tggaaagacc ttcgcacatc accagttgac acactggtca gatggcagga gcaacgcttg
     2581 ttgtggttgt taatggccgt tgcgatgggc gcgttaatta tcctggcgca ctctttcttc
     2641 cagatctatc tctacatggc accctgtgag caatgtgtat acattcgcta cgccatgttt
     2701 gtgatggtta ttggaggatt ggttgcagcg atcaacccaa agaacatcat cctaaagcta
     2761 attggctgcg tgatggcatt ttacggcagc attttgggac tgaagttttc gctcaaacta
     2821 aacgatatcc accatgcagt acataatccg gatcccgatt cactgtttgg cgtacagggt
     2881 tgctctaccg atccaacttt tccctttaac ctgccgctgg cacagtgggc accaaattgg
     2941 ttcaagccta caggggattg tggctatgac gcaccaatcg ttcctgatgg cgtcacactg
     3001 agcagcacgc aacagtggtt tgtggaaatg tatcagcaat ctgaaggctg gtatctgcta
     3061 ccaccatggc attttatgaa tatggctcag gcttgtatgc tggcttttgg tatgtgtctg
     3121 gtgctgctgg tcattatgag tggcgcatgg gcgcttaaaa tcgtcagagg ctaactcagc
     3181 aacacccgaa gccagtcgtt ctggcttcgg gttcttttat tagcctatct gcaccgacaa
     3241 catacttaaa ctgcccattt ctataccatc gaccggaatg gtaattggct cctgcccatc
     3301 ccacgcacct aagacataca acaacggcag atagtgctct ggcgttgggt tcgataacgc
     3361 gccaccttca tggtcgagat aattcaccag aggatgctgc tctaccggcc cttgccacgt
     3421 cagattcgct ttcacatact cattaaacga cgtcgcccac ggatacggtg aactatcacc
     3481 gtgccacttc actgtgcgca ggttatgcac cacgttaccg ctggcgacca acattattcc
     3541 ttcatctcgc agcgccgcga gtttgcgccc catttcgaaa tgccaggcgg caggtttgct
     3601 actgtcgata ctcaactgca ccatcgggat atccgcgtca ggatacatct taatcagcac
     3661 gccccaggaa ccgtggtcaa agccccaggc ttctttatcc agcgtcaccg ggatcggcgc
     3721 taacagctca accagacgct gtgccagcgc aggcgaaccc ggagcaggat aatgcgtatc
     3781 gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt
     3841 cactcctgtt ccacgggtaa accagtgagc cgaaaccacc acaatcgctt gcgggcgtgg
     3901 caatgtcatc cccaacgtct gccagctgcg ggtatacaaa ttatcttcca gcacgttcat
     3961 cggactaccg tgacctaaaa acaatgctgg catacgtgtt gaagacatga tgatatcctt
     4021 aactaaaggt gtcattttga tatcctcaca atacgcttgt tcggcggagt aagaacccgg
     4081 ataacaatga tgatgatcat cagttatttt gacgatctgc ctgaaggtga agatttataa
     4141 ggagttgtcg atgtcagtac ctctcattct gaccatactg gcgggggcag ccacgtttat
     4201 tggcgcgttt ctcggcgttc tcgggcaaaa accctcgaac cgcttactgg cgttttcgct
     4261 aggatttgcg gcggggatca tgttgctcat ctcattaatg gaaatgcttc ctgccgcact
     4321 agccgccgaa ggaatgtcgc ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg
     4381 ctattttggc ctggaccgca tgttgccaca tgcgcatccg caggatttaa tgcaaaaatc
     4441 ggtgcagccg ttgccaaaat cgatcaagcg cacagccatt ctgctcactc tcggcatcag
     4501 tctgcataac ttcccggaag ggattgccac ctttgtcacg gcgagcagca acctggagct
     4561 gggctttggc atcgcactgg ccgttgcgtt acacaatatt cctgaaggtc tggcggttgc
     4621 aggcccggtt tacgcggcaa cggggtctaa acgcaccgca attctttggg cggggatttc
     4681 tggactggca gaaattcttg gtggtgtgct ggcgtggtta atcctcggta gcatgatttc
     4741 cccggtggtc atggcggcaa tcatggcggc ggttgcagga attatggtgg cgctctcggt
     4801 tgatgaatta atgccgctcg ccaaagagat tgaccctaat aataacccca gctatggcgt
     4861 actatgtgga atgtcagtga tgggattcag tttagtgctg ctacaaacgg cgggaattgg
     4921 ttaaaagcaa catcagaagt tgtccagcgc atggacaact tctgccagct tcactaaagc
     4981 cttattctac cttttcggct gtctcttctc tcgtactgtt taagtatttg ttccgcgctt
     5041 caagcagtgc gtctctgtcc tggcgaaacg cccgacaata acgtttcaaa tgcaggaaat
     5101 aacccaccac aacaagagca atc
//
LOCUS       DAZGAP010000268         5081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGAP010000268 DAZGAP010000000
VERSION     DAZGAP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.269"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..1177)
                     /locus_tag="ACHLAM_004858"
     CDS             complement(107..1177)
                     /locus_tag="ACHLAM_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7856253.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVVGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSTVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
     gene            complement(1188..3710)
                     /gene="lpfC-O113"
                     /locus_tag="ACHLAM_004859"
     CDS             complement(1188..3710)
                     /gene="lpfC-O113"
                     /locus_tag="ACHLAM_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO7856254.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            complement(3735..4466)
                     /gene="lpfB-O113"
                     /locus_tag="ACHLAM_004860"
     CDS             complement(3735..4466)
                     /gene="lpfB-O113"
                     /locus_tag="ACHLAM_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO7856255.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(4514..>5081)
                     /gene="lpfA-O113"
                     /locus_tag="ACHLAM_004861"
     CDS             complement(4514..>5081)
                     /gene="lpfA-O113"
                     /locus_tag="ACHLAM_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO7856256.1"
                     /translation="RNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTA
                     DQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDLKV
                     GTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKF
                     IARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         1377 a         1200 c         1112 g         1392 t
ORIGIN      
        1 ctaagaaaaa gaaaagtaat gtcatctttt tgtcatgcag agtgaaaact cgcggacaaa
       61 aatgcgtaag atgacaacgg tgtgaggaac accgctgtca gttcgtttag cgtacaatct
      121 caattgttat cgttgcagtc gcggtaaatg ttcctggcgc ggggcgagcc cccgtaaggc
      181 ttgcaggagc agcagaaaaa gtgacgctac cggctttttc accaaagacg gttgatttac
      241 ccatatctgt cggcagttca ccaccgttaa cattgatttc cttattattt ttatcataaa
      301 ttttaatccc aacgttcggg tttgatgtca cgaccattga gttgtcagta ctgggttcgg
      361 tagcataaaa agaggtactg atatcatcag tatttagtat tcctaaattt tcacactcaa
      421 tggtgacggt tttggtaatt ttatgcgttg tcacggtact tccaactacc gaagaaaaat
      481 ctgccgcagg aatttcttta aaatcaaaat taatgggtct taaactgtta atttcacagc
      541 tttgcggtgc agtgatatcg ccacgaatat aaacatccgc cataggttgt gagtaatcaa
      601 ccggagtgtc acggcttatg gttccgtaca atttcgccac gagagtttca ggaataatta
      661 actttccagc aaaagtcttc ttaatataaa aataaattat tgcttttgca ccactggaaa
      721 tacttaatgg gtccgtgcga ctacatttat atgaacctcc agtaggcctg ttagcatggt
      781 attcaaaagg tgcattaatg tatcctacac cgagaaggtc tatcgatatt ccaacatcca
      841 gattaggatt caaattaaag tagtttacat tagaacgaac acctgcataa cctaaggact
      901 cgtttattct cgctgtataa taaatataag gaaactctcc cacgttccct gtatcacaat
      961 ggcagtgagc aggataagag tccccatttg tcacggtttt attaaaggtc tttcccactc
     1021 tgttctcgtc tgccgtaatc agatagccgg aaaaatcaag tgtgctatga aaggacccat
     1081 tatctgaagt gcagatacct tttggggccg caaatgttgc gccactaaag gagaagataa
     1141 aaacaataaa gcaccatggt ataatatact tgttcataaa aatcctttta atgacatgtc
     1201 acaccttgtt caattaagcc gctttcactt tctggtattg aaagtgaata atcgaaggtg
     1261 catgtagtgg cggcattttc tccccaggag agcatcacct gccctttttc aggaaggccc
     1321 gagagataaa gctccccctg gtcaccgaca attcctgaat cagcggagct tgtattttgt
     1381 actgtagcaa tagcgccaaa aggtgctggt ttaccttttg tacccgcaac cctgaaaagc
     1441 acacgataac caacacgagt aacaaattcg gccttaacca ctgcaccacg ggtgggaacc
     1501 atcgtaacga cggtattatc aagttctata ttcgacttcg agaaatagtt atcgctgatt
     1561 gctacccggt tttcatcata agggtttagc tgtgtcttga ccgtatagcc gcgccagtcc
     1621 gtcgcagcac cgcgcatatt gtctatttct aacccggcag cccctggtgc tttaacaaga
     1681 gctatggttt cacccaactc ctggcttaac gttacgcctt cgctgtgcgc cagaattccc
     1741 cctctgatac cataattcag cctctgagaa ttttggctgt agttatagct gccgttcacc
     1801 gtgccataag tgccatcata ggtcagggac atattaccgc caacctcatc atcatggctg
     1861 ttataggact gcattaaatt ccaggaaagg ttatttcttt ccagcgccgt accgctgagt
     1921 ccaagttgat gtgagctacc ggaattatcg ttttgtgaaa cagaataggt ggcgtaacta
     1981 tttccccacc agcccgataa aggaacagat aaagagagca tgtagatctg ctcactggtg
     2041 ttatcactgg catggttgtt ccccgtcccc tgccgggcag tattggaata actccaggag
     2101 acgttccacg agagacgctt ccagctactg ctgtagccaa attgcattaa tcgttcaaca
     2161 tcatcggtat gccagtactg ctggcgactt aatgtcagat ataagttgcc atagtctctg
     2221 cccagtgatt gcgttaacgt tccttccaca cggctgcggc ggttaccggt ttcccagaaa
     2281 tcttcaggat tattacgccg cgacgcccat tcattgagag tatagtaacc ttcggtggag
     2341 taacgatagc caaccaggcg caagctggta tccgtatcgt caaagcgttt cgaatacaga
     2401 aagcgataag actgaccggt ttccgtatca gcatggctaa atttcgcacg ggcatgtgtc
     2461 acatcgaaag aaattgcacc cagtgcgcca agctctttac cgacacccaa agcggcagaa
     2521 ccatactttt cagcagcctg catacctcca tacaaggtga aaccgtaggg aaaaccatga
     2581 aggatctgca cctgaagaac gtcaggggta aagccatctt catcgcgtaa ttctcccaca
     2641 ctgacatcaa catcagtttg accttcacgt ttgaggatgg ctaacgacgc ataaggttga
     2701 gtaaatcgtt gttcgctacc gtcttcttct ttgatggtta cgtccagatc accacctgta
     2761 ctggcggtgt tgaggtcggt aatttcaaaa gcaccttgtg gaacggctga ctgataaatc
     2821 acgtagccat tctggcgaat aatgacagtg gcgttagact ttgcaatccc acgcactacg
     2881 ggggcaaatc cgttttggct ggcaggcaac atatcgttat cggtatacaa gcgtgcgcca
     2941 cgaatttgcg tactgtcgaa gatatcgctc gccgtccacg tatcacctat cataatctgg
     3001 cttcttaacg cagctatatc acgttgtatc caggtgctta tagagttgta ttctgcactg
     3061 ccatcggtcg tatttaaggt gctgtaattg cgtaatcgcc acggcccaat attcagtcca
     3121 ttgcgcagat tgagataact ggtatctgtc tcttgtgaac cataatccga acgtagagta
     3181 tgtgaaccag aaaaatcgta gttgatggtg agcgcgttaa tcccctcatc ccaacgcgag
     3241 gggctgatgt aatcataagc catcgcatcc atatagattt ggggaatgga gatattcagt
     3301 tgcagtttat cgctgtcgaa ctgccatgat gagccttcaa tgcgtggccc aggatcaata
     3361 cagactgttt catctacatc attgagcgat ttcagcgcat caacttttac gccaaaggtg
     3421 gttagcgttt ttttaatagc gacaaagcag gcgtgtaaat cagcatcggg attatcctga
     3481 acaaaggtca catcctgacg ttcaaacatt ttaccgttga taaaaatatc aacgctataa
     3541 acaccagggg gcatcaggtt tgcccgatta aactggcgaa tatcgactcc ggattgatgg
     3601 tcgatttcca gaagcgccgg attgaataca ctgacctcgg ccatcaggct ttgcgagaaa
     3661 atcagacacg tccctgccgt gaggccaacc actattctgg tcgtcatcat aatacgccta
     3721 tccttatccc ttgcttatag cgtcattttt atcgggtcac tgactgcgcc gtaatcatta
     3781 ataaatcgcc aggtcatttg acctcccgca gaattagcag gtaaaggtaa cgtgagcgtt
     3841 tcaaaaggac ggatcatgtt gaggggcaca tcatgaccat caacgatgac atccatcaca
     3901 ctgatatgaa tcgggttggg attctctgca atcaggtttc gcccttcccg gtgccacttc
     3961 accttcggtg cagcatcttc gggaatcagg cgaatgcttg ccggtcgcca gaacagcttg
     4021 atacgcgttt taactgcgat ttccagtgag ttcgccgctt tgttgctcgc agagggcgga
     4081 atatttttaa tattcagcca gaagactgac tcgcgatcat caggcaatga cacacctttg
     4141 gtatgaataa tgcgtaatac tttttcttgc gcgggttcta atcgtgatac cggaggaata
     4201 acggtaaacg gtgctttgct tttatcctct ttattaaacg gatctaccca tgcctggatg
     4261 agataaggaa ttttttcttc cttgctaaat acagaaatag atttatctgg gttattactt
     4321 aagtaaacta cccgtgtacc accaatgaca acgcctgcat aggttaatgc tggttggact
     4381 aaaaaaacac tgagtataat cgcggtaaat aacttacatg aaacggttgt tttaaagagc
     4441 ctgattaaac aactttttct ggacataacg actccctggc caaagccaac tgaataaaaa
     4501 ggcccttgat aaattatttc ttatattcga cagtaaattg tgaatcggcg ttcgccgtac
     4561 ccgccgttac ggttgcattg gttgccacat agcgtgcaat aaacttcatt tcagccttgc
     4621 cttcagcgtc gatagcggta taggcagaat cgttataaag tttaacctgc gagttatctg
     4681 actggttaaa aatacgaata ccgacacccg ttgccgcaat cgcttgtcct gtaccggtaa
     4741 aatcgccagg gttgcccgct gtaagcggcg tacccacttt caggtcgccg tcgccgccag
     4801 gcgcttctgt accatcaaaa cgaacggctg cctgagtgta ggttgccggg cacttttcaa
     4861 gtttaatact gaactgctgt ggtgacgcca gactacctac acctgaaaat gcatttttac
     4921 tgactttacc cagtgggacg gtttgatcgg ctgtatcgga ggtaacttca caagtcgtgt
     4981 cgacaatttc accgacgaag tgaacaacac catcttctgc cagtgcatgt ccggccagca
     5041 ttaaagatgc cagagtgaat gcaccgccta taatattacg c
//
LOCUS       DAZGAP010000269         5060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGAP010000269 DAZGAP010000000
VERSION     DAZGAP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.270"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(183..339)
                     /locus_tag="ACHLAM_004862"
                     /pseudo
     CDS             complement(183..339)
                     /locus_tag="ACHLAM_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(539..964)
                     /locus_tag="ACHLAM_004863"
     CDS             complement(539..964)
                     /locus_tag="ACHLAM_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7856257.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIELEMDRYVERERSGAQVSQFSGHTGN"
     gene            complement(1043..1906)
                     /locus_tag="ACHLAM_004864"
     CDS             complement(1043..1906)
                     /locus_tag="ACHLAM_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856258.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKSSSVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1906..2874)
                     /locus_tag="ACHLAM_004865"
     CDS             complement(1906..2874)
                     /locus_tag="ACHLAM_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO7856259.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2876..4534)
                     /locus_tag="ACHLAM_004866"
     CDS             complement(2876..4534)
                     /locus_tag="ACHLAM_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO7856260.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            complement(4568..4936)
                     /locus_tag="ACHLAM_004867"
     CDS             complement(4568..4936)
                     /locus_tag="ACHLAM_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856261.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT         1202 a         1402 c         1108 g         1348 t
ORIGIN      
        1 ggcagcataa aatcatcctt ttatgaaaat caacgttttt tgagcactcg cacacaaaaa
       61 attaccgcac gggttgcaac gacaacctgc attttgcgga gcatatgaca aataaagtac
      121 gggtgcgttg aggatgcctg acacatcaga ggtggcggga gattactccc ccgcctggtc
      181 tcttacttct cagattcgta gtctacgaag acagcgacct ccgtctggcc ggttcggatc
      241 cgtacctcgc agaggtcttt cctcgttacc agtgccgtca ctatgacagt taaacagatg
      301 acgatcaggg cgaattaaca tcgccttttg ctgcttcata gactgcttct ccttgccttc
      361 cggcacgtaa gaggctaacc tacggttgtg tgacatagat tgggcctcag attaatgtta
      421 agcgtcttgc aggacacgta atgttaactg gggcttttct ccgtctgcct tacggcagca
      481 tgcccgaggc agacaccctc aagcacccgc agtcattcta cagtaaaacc ccgcgctttc
      541 aatttcccgt atgtccggaa aactgactga cctgtgcccc tgatcgttca cgctcaacgt
      601 atctgtccat ttccagttct attccctgca taatcaacat cccctcaata acaccttcag
      661 ctttttgcag tacccgcccg gcccagcaat cagagcgctc atgcttacgg cccagtccca
      721 taagcgtcat cccgaacaca taataatcat acagaaaatc atgcaaatcg ctgttgttga
      781 cgttcagttg cgccatgcaa ttgctgataa tcagcgcatc atcatcggta cactgaggcc
      841 gtgatttaac ctttgatggg atcactccct taaatccggc agcgacgccg gaccatgcca
      901 cactctcatg attatttacc acccatgcac cccatcggga cagaacctgt tggatatcac
      961 gcattattct gtactccgct gtaaccatac tttttctgat ttgaatgctc agggggaaaa
     1021 tgtctactgg atatctccgg cttcattgct cacactcccc aaccagattg agaatgaccg
     1081 ctgaaccatt gtcttccatg tactcatctt ttccgcttgc cagaaaccag cgacacactt
     1141 caaccgcttc atcacgcgtc acaggcttta tttttcccag caaattttcg agccagaact
     1201 gccgatcata tactgactca tgatgttcac ctgtgaaacc gtatttgtcc cctgtttctc
     1261 tccccgctgt ctggcgcatc tcataaagcc agtcccagta aataaactca cgaacaacgt
     1321 ctgacaatgt atgaggctct ggcagtacat cacgatagcc atcaacatat gcacgacgct
     1381 gatcatcaat ttcatttgag cgaacaccgt caatacttcc ggcttttttc tctgccgcag
     1441 tccagcccca gtagtaatcg tcgataaatt tcggggaggt gcgaattatc cgctcagcct
     1501 ccacatcgtc gagtgctgcc tcatagctgc caaatgtggc cctgactgat gaggattttt
     1561 tgatattctc acgggcattc ctgatcgcct gcgccgggtt atccatgcct atggttgcga
     1621 aagcaatctg gaaaggatca acattaatct cctgcaaata acgtccatac cgttcactcg
     1681 cttcttccgg cgttattttg agtttctcca gtgctgcttc ggctgcgtcc agatgtacgg
     1741 cctcgttcag gtgaataacc tccagaatcc agagataagc atcagtctgt ttatgtccgg
     1801 tgatcctcct ttgttctggc agaggtttga tatttgccgg aacggcgctg tacgctgccg
     1861 tcgggatagt gaataaagct ttatgtacct gattgtccgt acgcatcacg caaccgcctt
     1921 ttttctgtaa aaaaccagtt cccgaacctg atcgccgttc atgagcatat tgttgaaatc
     1981 atcatgatca ggccaccgga cacttacccg ctgaatgtca tttcgggcct ggagatttgc
     2041 atgtgcgcat tcacaggccg cagccattcc ggtagcactg tattcatcgc gatcggcaaa
     2101 aataatcaga tgggtgactc ccgccggtac gcggaacttt ttcataaaac cactgttaat
     2161 ggttgcccag gtatttactt tgtaaatctg atgtgcggac aacgcggttt cgataccttc
     2221 agctataccc agtgtactgg ctaccggaaa catcctgata gcaactgacc gggcatggtc
     2281 cagatagtta tcgtcctgta aagatttgag ccttctggcg ctgataccaa tgtctgcttt
     2341 tttagctcca tcaagcaatg tctgatgcag gtaacacagt tctcccttgt catcggtcgc
     2401 cagggcataa agagactgga acatgcgccc gttataattt tgctggctgt tgaagcgaac
     2461 tccctcaacc ggaagactga atatgccacg ggaacgaaga tattcagcac cagatgttcc
     2521 ctgtagcgcc gtgagcgctg aaaatcgacg ctgaacgcgc gatctcaggc tttccgacgt
     2581 acagatcaca ggcggtttat ctctccggta ttcattcccc gtgatgcggt ctatttcagc
     2641 gcaaatttca tgaaacgctc ttttttgtgt cagagcgatc agtttcatgc cgtcaccgct
     2701 accacaaaca cagatccacg ttccggtgcc atcacggtca tcaatacgga acttacctct
     2761 ggcaccacaa accggacatt cccctttgaa gtgcttttta cccgtgactg gcggcagtcc
     2821 gtaatgtttt aaaatttcag gccagtggcc tttcgctgct tctgctgttt tcatcttact
     2881 gactcagact gtttctgttt ttctgaagtt gttgctttgc ctgcatgatc tgccacgact
     2941 cactgccttc cggcacccca gaccggacac catgaagcag ggacagagaa agcgcttcgg
     3001 gggatttttc cgtcatcttc tctgcccggc tacgccctct ggcaaaccgg atgaatttgt
     3061 gtctgatgta attactgacc tccggggtga tttccatcgg aaaactgctc agcccgtcag
     3121 gccattcgcc gaacttctcc cggaaagtgt gagcgcacca tccatcgctg accggtcgcc
     3181 ccagagaggc acgctgacgc tgatagaatt tgatctgact ccaccaggcc tgtttgtcgc
     3241 ttttggtgta aattttttcg tccttgctga gtttcctgat attgcggcgg gtatctgtgt
     3301 tcacatcctc accagccaga ggtttaaatc cgcatttcgg acagacataa acacctgccg
     3361 gtttcatgaa atgacacccc ggacattctt tcggcagttt ttcttcccga atttcgttac
     3421 cacgtgatga tgacacctgc atcccgtcgt ttttgccggg cagttcgtca tactcaatat
     3481 tttcaggaaa tcccaggcga tgaaccgtac cggaatggtc gaaaatcagg catgactcct
     3541 tgcctggcgc tgtacgcaat cctctcccaa tcgcctgaag ccagcgaatc tctgattttg
     3601 tcgggcgtgc gtaaatgatg caccggacat cactgtcaaa tcccgccacc agaacgccaa
     3661 cgctgacaat gattttcgtc gctcccgttt caaaacgact gatcattaac tgacgctcat
     3721 catgtggcgt ttcagcaacc atcacctctg cgttaattcc ggctttgttg aactgcatgg
     3781 tcacgaagct ggcatgcgtt ttattgacgc agaacgccac tgtcggtaaa tcccttccgt
     3841 tacacagcca gttatcgaca atatcgccaa ccagatccgc gccgcacata atctctgcca
     3901 gttgtgattc gtcgtaatcc ctgccgtact ccattgagac tttcgttttt acaccttcca
     3961 gatccggttt cgtgggcgca taaaattcgt aattgctcag ttcgccacgc tggatcagtt
     4021 cccctatagt ggtcggcttg agaagacgct ggtaataacg ccccaggaac gatgaaaagg
     4081 gtgtaccgga caacccaatc accttcacat ttccctctgc aaccagtcgt tcgatttccg
     4141 tgaggatgcg gcgcttacgg agatgcgctt catcaataat cagcaggtcg atattctgag
     4201 gaaaatcacg gcgaatcagc gtatccgcac tggcaatctg aatcagtcgt gacgggtcgt
     4261 tattgggatg atcacgccag ataaagctga tttcatcccc ggaaagcccg tactgaacaa
     4321 agcgctgtgc tgtctggttg atcaaaatgg tataaggaac gacaaacaga acgcgcatgc
     4381 cacggcttac cagaccagca gtgatgaacg ccgccaggcc ggttttacca ctccctgtag
     4441 gcatgtacat catgaaggac tgatatgttt tccagtcatg acgcaacata ttcagcgcac
     4501 gttcctgagc aaaattcggc ttgatattca gcatggtatt ctcccgatga cagggctgat
     4561 acagtgatta cttaaaccgg gtcgcccgga tgacatccac accatcggcg atataacgct
     4621 gtggatgttt gccatcacgg agaaaaacaa atcccgaaat gccttcagga agcgctcgaa
     4681 ttttcatcag tgaacgctga cggggcgatc ggttatccac ctcaactgcg caacgctgac
     4741 catcgggaga aataaccaga agatcgataa atcccctgcg atcacctttc agcctgacag
     4801 tgaaacagcg ttgcaactca cagcccaggg atttgatttt cttttgcaga acgtagtcga
     4861 atgtgacctt gtccattcca tgtccgaatt cctcttccag aaggttgatt agtaccagtt
     4921 tcagttcagc gctcatattt taaccatgat gtactagttt gctgatacga ccgttttttc
     4981 aggggtgccc ccctttagat cgagatctac ctaacctctg tacccgtctg ttggaagatc
     5041 ccgttccagt gcttcgcact
//
LOCUS       DAZGAP010000270         5024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGAP010000270 DAZGAP010000000
VERSION     DAZGAP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.271"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(4..762)
                     /gene="aroD"
                     /locus_tag="ACHLAM_004868"
     CDS             complement(4..762)
                     /gene="aroD"
                     /locus_tag="ACHLAM_004868"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO7856262.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDIIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(793..1659)
                     /gene="ydiB"
                     /locus_tag="ACHLAM_004869"
     CDS             complement(793..1659)
                     /gene="ydiB"
                     /locus_tag="ACHLAM_004869"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO7856263.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1671..2936)
                     /locus_tag="ACHLAM_004870"
     CDS             complement(1671..2936)
                     /locus_tag="ACHLAM_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7856264.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(3163..4377)
                     /locus_tag="ACHLAM_004871"
     CDS             complement(3163..4377)
                     /locus_tag="ACHLAM_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7856265.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALLLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(4477..4833)
                     /locus_tag="ACHLAM_004872"
     CDS             complement(4477..4833)
                     /locus_tag="ACHLAM_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO7856266.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTDDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4969..>5024)
                     /gene="rprA"
                     /locus_tag="ACHLAM_004873"
     ncRNA           complement(4969..>5024)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHLAM_004873"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1459 a         1150 c         1150 g         1265 t
ORIGIN      
        1 tgcttatgcc tgatgtaaaa tagttaatac cgtacgcaaa tcatttaccg agatttgccc
       61 tggcgcagag gcttttttta ccgcaccaaa agttgccgcc gagccaaata cttcaccagc
      121 cagacgagaa attacgccag tttttgccat cgacatcgtg atgattggac gatcggcata
      181 ctgctcctgc atctccaggg tcgcagcaag caacgtcagc acatcgctgg tactttgcgg
      241 catcagcgca atcttaggaa tatcggcatc gaaggactgc attttgcgca gacgggcaat
      301 gatttcttcg gcttccggcg ttttatggaa gtcatggttg gacatgacaa ctttcacatc
      361 atgcgcgtgg gcgtaggcga cggtttcttt aacctgatca tcgccggtaa ataactccag
      421 atcgattata tcaaccaggc cgctgtcgat ggctgcacga ttgagtgcaa tataagcctc
      481 ggtggaaatc gcctgctcgc cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt
      541 ttctggcatg gtttcacgga gaatttttgc tgccgctatg acagactcca cattggagag
      601 atcggcatag tggtccacac gccattccag aatatcaaag tccgcttcac gataggcgag
      661 agcttcggat ttcacgctgg cgatatcttt cgccatcagc gagacgatga ttttaggtgc
      721 gcccgtacca atgacgagat cttttacagt tacggttttc attttttacc ctttctgcac
      781 gcggtcagcc tgtcaggcac cgaaccccat gacctgttta acatattcca gagggaaatc
      841 tttgccagtc cataacgtga actgttcagc cccttgccac aacaacatgc cgtatccatc
      901 aatcgttttg caacccgctt gttgcgcctg ctgcaataac ttcgtcatat gagggttata
      961 cacgcattca gtgaccagaa gtcccggatg taacagactg atatcattaa ccaatgattc
     1021 attctcaagg ggtttcatac ccactttggt gccattggtt aaaatgtcgg cggaagccag
     1081 ggcttctgca aaggcttgct gatcggcgag atcggtgact gtcacgacgc aatcggtgtt
     1141 ttcgttaacc cgctgcgcga aggcgagagc tttatcgaag aactcatccc gacggttaaa
     1201 gagtttaatt tcttttaaac cttcaattgc cccctgcgcg ccaattgccg ttgaggcacc
     1261 accggcccct aatagcacca tcgttttgcc tttgatatca aaaccgctct ctttaatggc
     1321 gcgaatatga cccgtgccgt cggtgttata gccacgcaga tagccatcat cattaacgat
     1381 ggtgttgatg gctcccacca gtttggccgc tggcgttaat tcatcaacat attcacacgc
     1441 cagttgtttg ttcggcattg atacaccagt tccgcgcatt ttgagggctt ttaatccttc
     1501 aattgctccg ggaaaactat cgttatccac ttcaaaggcc atataggtaa atggcaatcc
     1561 cgctttttct aaggctttat tctgcatttc gggcgataaa ctgtggcgga tgggataggc
     1621 catcaaccca atcaattcgt attttgcggt aacatccata attaactcct ttaacctcta
     1681 tgcttaatcg tgtttaaccg ggtgctaaat ttatgctcgc caaaccgcac atcattttca
     1741 ggaataatga aaacgcggta atagcggata aaaacaataa ttgcggtaat cagggccagc
     1801 agcgcgaaag taaaatcgag aacaatgata tattgcaggc cgatgttcga cagataaccg
     1861 gtaatcagcg gaataacaaa gttagccagt ccacccatca tcatatatat actggtgact
     1921 ttggctttgc tttttgggaa aaactctgac atgaccgaaa cgccgagctg taaaatgccg
     1981 ccagctgctg agaaaccgat aacaaaggct ccggcattac acaccaatgg agaagggtac
     2041 aggtagataa tggcagctgt tattgttgcc agtgcagagt taaatacatt agcccagatg
     2101 ggccggacca tttttttcag cagggcggca aaaataaaga cacagaccag cgaacccata
     2161 ctgtaataag agatggtttt taatgcctca gcttctgaca taccagcaaa agccatcgca
     2221 tatttgggca tccacaccac aatcacataa aaggtcgcga atgcggctac accgaacagt
     2281 actgatgaaa caccttccag ccagactaac ggtttgctgt tcatttgcgg taattcatta
     2341 gctacgctgg cgtccaccaa ctggctgggg aatttgcttt tcaacagcat cagcgtgatc
     2401 agtacaaaca gaatacccgg aataatcagc ccatagccgt accagatgtt attgagcaac
     2461 atatagctca ccagcattgg gtagaacatt tgcccgaatg acaccatcgc tttaaccagt
     2521 atgaccgccg aaccagaggc tttcggaaag cattccataa gcgcggggta gccacccgta
     2581 tccagcgctg agttagcgat acctacgcac actgccagac agtaggcgag agttaaattc
     2641 gggcaagcag gaataccaaa gaagaatagc agatacatta ttactgccat taatatcacc
     2701 gcccgacgac caaacttatc ggagatcaca ccgaagaata aaatactgat caatcgcccc
     2761 aatccgatac cggaaattaa ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc
     2821 aaagatgtca tgttttgggc aagcgtaata acactaatgc cgtgcaggaa atagctgaag
     2881 taaatacaaa gaacagccag gataaatggc gtgctgaaag ccttattttg agacattttc
     2941 acagctcctt gctgaaagat aatttcgtta ccgtacgtat tgttctccga cggtttacat
     3001 aattactata ctgaagataa ttttccctgg gtacatggca taaagtttat gtttccagga
     3061 atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc agaaagagtt
     3121 atttatccgt aagatcttgc atgacctgaa aagaatattg cattacccac ccggagcgac
     3181 attttccttt agcgagtgat gccgcgtttc tttgcgactg cgtaagccaa taaacagtgc
     3241 cagtataaag ccgatggcag cgatggcggt atcgaaccac ataatatcgg caatacttct
     3301 ttgcgaaaga tgagccgtaa tcaacggaat agtaaaggtt gcaatgctgc ccgcactgta
     3361 atagatcccc gttgcttttc ctttagcgta gggaaaacgt tccgccatta acgtcaggcc
     3421 aatttgtaca acaccgcctg cggaggtaaa accgatgacg aaagcaaata ttatcaccac
     3481 ataaaatgtg ggatgcaggc agacggtgag cagggcgata aatgagataa aggtgtacag
     3541 catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa taaatacaca
     3601 aagcagcgaa cccacggtgt agatactgag taatttgatc gacatggtgt atgacatgcc
     3661 ggcgacaaat tgtccgtatt gtgccagcca ctggctaacc agataaaacg ttgccattga
     3721 gatatagcca tatagggaat aactggctaa atcgataatt gaacagcgat gttccgtaga
     3781 gctggtggtt ttctttatga caggtaagtg acgacccgga tggggtggga acgtacaacg
     3841 gtataaaagc agagcgttaa taaacataat gcctgcggca atcataaagg accaaccgaa
     3901 ccacagttca gcccacacta acaggctgat aattagcggt aataaaaatt gtccgctgga
     3961 aacaaatgct ttaattaaaa tattggctgt acccggtgag cgtggaaaag cttccatcaa
     4021 actgggatag gtgcctgcat cgagaaaact gtttgccatt cccgccagaa agccaaaaac
     4081 ataagcgata atgatgttat tggtgtgcag gatgccaaaa aagaaggcca tatagcagca
     4141 catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag
     4201 caagacactt aatcgaccaa tgcccagtga tgagataact atcgagacgc ccgcggcatt
     4261 agtctgccaa agcgtctcca gcgaggccat attcaggctc atcaaaatga cgcccatacc
     4321 atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat ttttcatagg
     4381 ttctacttag caaaggtgct ttaaaaagtg tagagatgtt gtgtcagtat ccgttcatta
     4441 ttgttgtaac aaagacaatt cctttgtgtg gagaaattaa caaagccgct ccagatcgtg
     4501 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
     4561 gtagacttgt tgaaacacgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
     4621 gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac gcatagccaa
     4681 cagttgctcg actacacgat caggaatggc gcatttgcca ttttcccact gacgccagct
     4741 ttcactgtca tcggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
     4801 atggcgaagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
     4861 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
     4921 caacattatt ggaaagcgtg aggggcgaga tagcaaagcg gaaaaatgtt aaaaaaaagc
     4981 ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgt
//
LOCUS       DAZGAP010000271         5022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGAP010000271 DAZGAP010000000
VERSION     DAZGAP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.272"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..589)
                     /gene="sixA"
                     /locus_tag="ACHLAM_004874"
     CDS             complement(104..589)
                     /gene="sixA"
                     /locus_tag="ACHLAM_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO7856267.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(792..2936)
                     /gene="fadJ"
                     /locus_tag="ACHLAM_004875"
     CDS             complement(792..2936)
                     /gene="fadJ"
                     /locus_tag="ACHLAM_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO7856268.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSGGYDAEARAFGEL
                     VMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(2936..4246)
                     /gene="fadI"
                     /locus_tag="ACHLAM_004876"
     CDS             complement(2936..4246)
                     /gene="fadI"
                     /locus_tag="ACHLAM_004876"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO7856269.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLLDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEMDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4427..4711)
                     /locus_tag="ACHLAM_004877"
     CDS             complement(4427..4711)
                     /locus_tag="ACHLAM_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO7856270.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            complement(4796..>5022)
                     /locus_tag="ACHLAM_004878"
     CDS             complement(4796..>5022)
                     /locus_tag="ACHLAM_004878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856271.1"
                     /translation="SVGSEVHLRNQTSGESIGRTSWNVASMFLSFFRYDLRNAIQLAK
                     LGGGALMDSGEFCFRSFLSVISVTYPILQN"
BASE COUNT         1241 a         1396 c         1200 g         1185 t
ORIGIN      
        1 cacaagcgga tcattgttag tacaataaga ttgcataaat tcaacattgc cagaaaaaaa
       61 taaaggcccg atagattaca ccagcgggcc ttgtgcatgc gtattaaatc gcttttgcca
      121 tcttcagatt acacgggctc atctgccagt tgaatgtgcc gtttccgctt tcatcaagcg
      181 ttacgctggc aatggccgag gtggtaaaca taggcggcgt ttcgcccgga cataactcgg
      241 caaccagata cccgactaag ggtaagtggg agatcaccag taccgaagca acaccttcat
      301 tggtcaacgc ctgcaaatag gcactgacca gaccaacatc gccgcagggc gttaactccg
      361 gcagaacttc tgcactggaa ggcaggttca gacaatcccc tacttcttca agtgtttgct
      421 cggctcgcag gaacgggctc accagaacgc gttcgatttc cactttttga cctttcagcc
      481 agttcgccat caggcgagat tcgtcacaac cgttagtggt cagaggacga accgaatcac
      541 tggcggcatc gagggctgcg tcgccgtgac gcatgataaa aacttgcata ttgcaccgct
      601 tttgttaacc agattcgccc gttttctttc atctgaaacc agagaaagaa aacggtggcc
      661 ggcattgtgc cttatccatt caccgaatga aacgctgttt tttacctcaa tggcgtaagt
      721 atagtcaata tgcgtttaca ttttgagcaa cactccgcca ttcagcgcgg attcatatag
      781 ctttgacctt cttattgcag gtcagttgca gttgttttcc aaaaactttc cccacgcttg
      841 ctcatctcga ctaaacgctc gcaaggggta aaacgggaac catactgcgt ggccagtcgt
      901 tgcattattg caaccacttc acccgcaccg agagaatcga tgtagcggaa cggaccaccg
      961 agaaatggcg gaaaaccaat gccaaatacc gcgccaatat ctccgtcacg cacgctacgg
     1021 ataacctgct cgtccagaca acgtactgct tcattcagca tcaacatcac acaccgttca
     1081 gctacctgcg gtgcggagag tcgcccctgc ccttgtgtgc caatgagcgg gtaaatggcg
     1141 ggatcgacct gttttttgct tttacgccct ttctgaccat aaagatagaa accccggcca
     1201 ttttttctgc ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta
     1261 aaacgttctc cataagcggc ttccagtaca ggaataattt tagtcccggt gtcgattcct
     1321 acctcatcca aaagttggat tgggcctacc ggaaaaccaa atttcaccag cgcggtatca
     1381 atgtgctcta cccgttcacc ttcggtcaac atgcggatag cttcattaat gtaaggcgct
     1441 aagatgcgat tgacgtaaaa cccggctttg tcacgcacga caattggcgt tttgccctgt
     1501 tttttcgcca gttttactgt ggtggcgatg gtttgcgccg atgtaccggc atggggaata
     1561 atttctacca acggcatttt ttccaccgga ctgaagaaat gcaggccaat aacttgctca
     1621 ggtcgcgcgg cgtgagcggc gatatcgcca atcggtaaag atgacgtgtt cgaagcaaag
     1681 atggtatgtg cggcgcaatt ttgctcaact tccgccacca tttgttgttt taattcgaga
     1741 ttttcaaaca ccgcttcaat aatcagatcg cgatgggcaa agccgcaata gtccgtcgtt
     1801 ccggagatta atgccagctg tctgtcacgt tcgctggctt tgagatgacg acggcgaact
     1861 ttgccctcca gctgatccca actgtacttc agcgcatgat ttatgccctg cgggttgata
     1921 tctttaattc tgaccggaag ccccgcttta caagcagtga cataagcaat accgccgccc
     1981 atcaagccac cacctaaaat tcccacgctg tttaatggcg caggcggcgc atcactgcca
     2041 ggatctttct tcacgtccgt actggcaaaa aagatactac gcagcgcctg cgattgcggc
     2101 gtcatcacca gttcgccaaa agcacgggct tcagcgtcat agccgccact ggtgccctgc
     2161 gctaatcccg tttcgacaac ctccaggatg cgttctgtcg ccggatagtt accttgagtt
     2221 ttgtgttctg ttttcttacc gaccattttg aacagcagcg cacgacctaa cggccccgcc
     2281 agaatacgct cgcgtacagg tagagggcgt gaagatgggc gatcctgctt tgccagctca
     2341 acagcggctt ccagtaaaat ggattgcgga acgacgtcat ccaccagccc cagctttaat
     2401 gcctgtttcg cccgaagttg ttttccagtg aggatcatct ccaatgctgt gctgacacct
     2461 atcaggcgcg gtaaacgctg ggtgccgcct gaaccgggta ataatccaag ttgtacttca
     2521 ggcaaaccga gcaccgtttt aggatcgtca gtacacatgc gaccgtggca cgccagcgcc
     2581 aactccagcc caccgcccag gcaagcgcca tgaatagccg cgataaccgg aacgggcaaa
     2641 gcatgaatct ccgccatcaa ctgttggccc tgccgcgcca gtgcttccgc ttcttgcgcc
     2701 gttttgcagt tggcgatcat gttgatgtct gcgccagcaa tgaagttgtc tggtttcgcg
     2761 gagataaaca ccacgccgcg caactctttg ttttcacgga gttgcttaat aatggcgcgc
     2821 acctgcgagg caaactccgc cttcagggta ttcattttct cacccggtac gtcgatggtg
     2881 ataacagcaa tgttgtccag acggacatta agggtaaacg ctgatgccat ttccattatt
     2941 ccgcctccac aaccattgcc gcgccaagcc cacccgcagc acaggcggtc actaaaccaa
     3001 atccaccgcc gcgacggcga agttcatgca atgtctgggt aatcatccgc gcgccggtcg
     3061 ccgcgaaggg atgcccgtaa gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca
     3121 tttcgccagt ggcatgtgca cgccccagta catcacgagc aaaacgttcg ctacccagca
     3181 actgaatatt cgccagtgtc tgagctgcaa aggcttcgtg catatcgagc aatgtcagat
     3241 cgctcattgt caaaccggca cgttccagcg ccagcggcgt tgaccaggcc ggaccgagca
     3301 acatgtcctg ccagacatct atcgcagtaa atgcgtaact gcgcagatac cccagcggta
     3361 ccagccctaa ttctttcgcc cgcgattcgg tcatcaggat caccgccgct gcgccatcgg
     3421 tcagcggcgt actgtttgct gccgttaccg tcccgtgttt acgatcaaac gccggacgca
     3481 actttgcgta atcagcaagc gaggaattac cgcgaatatt gttgtcttcg gcaagcggtt
     3541 gtttataagg agggataaag gcagtcatca cctcttcttt gagtttaccg tctgaccacg
     3601 cctgagcggc tcgctgatgc gaacggtgcg ctaatgcatc ttgctgttcg cgagtaatac
     3661 cgtaggtttt cgccatttgt tcagcggtgt cgcccatccg caagccagta gagtattccg
     3721 ccactgccgg aggcaccggc attaagtcgc gcaaacgcag gcgagagaag agtttcaggc
     3781 gctggctcat ggttcgagct ttgttgacat caaccagcac gcgcgccagt tttttactga
     3841 cgccaattgg caatacggaa gaggaatcgg ccccaccggc aatccccgcg cgaatagttc
     3901 ccgccatcag gctttctgcg acgtttgcaa tcgcctggaa actggtagcg caagcgcggc
     3961 tgacgctgta agcatcggta tgcacattca ttccggtacc gagaacaatt tcacgcgcaa
     4021 tgttgggggc ttcaggcatt tgtacgacct gaccaaaaac cagttgttca atcacttcgg
     4081 cggggatctc gctgcgtgcc agcagttcgc ctaccaccat cttccccaaa tcaacagcag
     4141 gaatgccatg aaaagccgtc gcctggcggg caaaaggcgt acgtaaaccg ctaacaatgg
     4201 cgatacgatc gccctggcgg gtaaccagcg gtaaaacctg acccataaca ctcccctgta
     4261 aaataataaa taaagtggtc tgacctgatc atagtcttaa ccattttttt acatttagcc
     4321 aagtggagaa aagggaaagt gggagctatg acacagagag aaaagtagaa gagaaaagaa
     4381 aacgcccctg ccagcaactg acaggggcgt atgtacccga gaggaattaa cgcagaccca
     4441 gctggaagat cagcatttca gcttcgcagg caaaagtgaa atcgatatcc aggcgcacac
     4501 cgtcagactc ttcagtgaaa gtcggggtga ttttgcacgg ttcggattcc acgctacggg
     4561 ctttttcagt cagcgccgcc agcgtttgtt ctgcttctgc gcggtttgca aacacacggc
     4621 tgtaagacgc ggtgcagtcg gagttgtcca taatggtgcc aacatccata cagcagcaaa
     4681 ccggggtttc atcagcacta catttactca tcgttgattt cctctgtatg tgcacccaag
     4741 gtgccagata aacgttgtgg atattttacg cttccggaaa gtgctgctcc agttgttaat
     4801 tctgcaaaat cggataagtg accgaaatca cacttaaaaa tgatctaaaa caaaattcac
     4861 ccgaatccat gagtgcgcca cctcccaatt ttgccagctg gatcgcgttt cttagatcat
     4921 atctgaaaaa agatagaaac atacttgcaa cattccagct ggtccgacct atactctcgc
     4981 cactggtctg atttctaaga tgtacctcag accctacact tc
//
LOCUS       DAZGAP010000272         5003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGAP010000272 DAZGAP010000000
VERSION     DAZGAP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.273"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..427
                     /locus_tag="ACHLAM_004879"
     CDS             92..427
                     /locus_tag="ACHLAM_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO7856272.1"
                     /translation="MNRKYYFNNMWWGWFMGGYTLYMSWDYEFKYRLLFWCISLCGMV
                     LYPVARWCIENTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLTLIYILS
                     VIIKRLSVR"
     gene            complement(449..1551)
                     /locus_tag="ACHLAM_004880"
                     /pseudo
     CDS             complement(449..1551)
                     /locus_tag="ACHLAM_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
     gene            1580..1855
                     /locus_tag="ACHLAM_004881"
                     /pseudo
     CDS             1580..1855
                     /locus_tag="ACHLAM_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2628..3605
                     /locus_tag="ACHLAM_004882"
     CDS             2628..3605
                     /locus_tag="ACHLAM_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO7856273.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3891..4631)
                     /locus_tag="ACHLAM_004883"
     CDS             complement(3891..4631)
                     /locus_tag="ACHLAM_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7856274.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGLRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4752..4973)
                     /locus_tag="ACHLAM_004884"
     CDS             complement(4752..4973)
                     /locus_tag="ACHLAM_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO7856275.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWYYPKND
                     KTALHPLHYWLHEGSEQKKNPFPIWFYPR"
BASE COUNT         1233 a         1204 c         1166 g         1400 t
ORIGIN      
        1 cttattgtta ttgattatta ttatcatgct gtggttatgt tgtcatgcat cagtacattg
       61 acaaaagtgc aaaagaaagg aaaacaggca gatgaacaga aaatattatt ttaataatat
      121 gtggtgggga tggtttatgg gtgggtatac tctgtatatg tcatgggatt atgagtttaa
      181 atacagattg ctgttctggt gtatttctct ctgcggaatg gttttgtatc cggttgccag
      241 atggtgtatt gaaaatactg ccctgaaatt tacccggcct gatttctgga acagcggttt
      301 ttttgctgat actcccggaa aaatgggatt acttgcggtt tatacgggta ctgttttcat
      361 attatctctt ccgttaactc tgatatatat tctttctgtt atcataaaaa ggctgtctgt
      421 aagatagaca gcctgtctta tatattattt aaatgcccca gaacttattc gctttttcca
      481 caagcgtttc atcaatcagt gcaccggtga cagccatcag taaaccatac ccgataatgc
      541 ctaaagcgct tccggtaaga atactgaaga ccagagcaac aagagccgtt gcggcattac
      601 ctgctatgat ggtttctgtt ttaacaaaaa aggacgccag ttatctgtcc gggcagcctt
      661 accaaactca gtgatccagt cagcaaaatt tgtaattttt cctgcatatc ccagtccgcg
      721 actgaacctg ttcagattag acgatatatc agacagcttc acagactcaa gggctgcggc
      781 aatcgctgca cgatcttttg cattaatttt tttgttaatg tcagtccggt acttttcata
      841 cgtttttaat gcctcttcaa cattacgtat tttcttccct ttagcctgcc cggccatctc
      901 tctggctaac tgctcagctt ttgcaccata tttttctgaa actgatttca ggaactctgt
      961 tgtgaaatta attgcgtctt tcgttgcctt cagttcatcc tgttttcttt tctcttccgc
     1021 tatcttctga ttgatgccgg caagctggtt acgggtattc tctttttcct tcaacagggc
     1081 attaagagcg tcatttgcat gcttttgctc atttgttctg gcactgaggt tatttctcac
     1141 cgaatttacc gcagattcag cagaggtgat tttatttctg gcatcaagca atctttgccg
     1201 taacttatcc cattcagtaa cttcagcatt ataaattttc ttgtcacctt ccacatcctc
     1261 tgtcaccacc ggattccggt cattcagaat attgcgttta tagtccagac cggaatgagt
     1321 cagaagataa ttcaggtttg cacggttatc gatgactgcg ctggttgtga tttttatcgt
     1381 atcggcaaat ctcggatcac cgctcacaac aatagaatga tttgttgatg aacgccccgg
     1441 gaatttttca gaggaaacta tcgttttgcc gtcaggcgtg tttttctgtt gcgtcagctg
     1501 ttgttcagct ctggttaatg cattacgggc cgtgttaagc gattccgtca ccgaactgag
     1561 tttattccgg gcctctgaaa tggccctgtc attccgtgtc tgctgctcga gcagacgggc
     1621 ctgttgtgca tcaagaacgg caagggctgc cccgcccttt ttttccagct ctgacaaccg
     1681 cgtattcaga ataccttttc tgcgctcgta ttcagcaagc atatcagcgg ccttcttgtc
     1741 tgcgttctgc gcatccttct gccgtttttc agcctgttca attaacgacc tggcctcgcg
     1801 gggagatata cgaagagaat cagcatcagc aaacagcatc cggctctctg tatgtgtcaa
     1861 cgccacgatg tttgaccgtc ttatatcact ggcgctgaca acacgacaga gttatcaggc
     1921 atggtgtcaa tcagttcaca atttttccat cggagcagcc taccaccggt gataacctga
     1981 ctttgcctgt agtttgtata cacaatccct ggcattgaaa cacgcatatg aggtgtactt
     2041 cggaatatga ccgtgcgctc aggaagtacg gcagattccg tcaaaaatta cctgtaaaga
     2101 cagacttcag aaaaaacagt gctcgccgca gaccggtgac cgggcgaagc ccgcgaaccg
     2161 gcgaggaagc gcaatatatc tgtagttaca atgagcaggt acgccctgaa gccttcataa
     2221 tcggtagtgt atgttgtttt tctggaatga tttatccaca tatctggagg tccgatccag
     2281 taaacagatc catgaatgat caacaaagga tccattaaag atccccatat cgctgcaaac
     2341 cttgtcactc atgggccggg accacgatca cataagctgt ggcatgttac tgataaactg
     2401 taacatgcta atgataagct gtattcagta atccatatac tgaagtaagt taatgacata
     2461 aactatggtc agtacgtcag actcagttgt taaatacagg ctgcaggttt ttattcagtc
     2521 agttagcgga gttctgacac acgattttct gtttattctt ttactgtcca caggctggag
     2581 gctttctgga aaacgaaaat tcagacatca aaaaactgtt cggcgaggtg gataagtcgt
     2641 ccggtgagct ggtgacactg acaccaaaca ataacaacac cgtacaacct gtggcgctga
     2701 tgcgtctggg cgtctttgta ccgaccctta aatcactgaa gaacagtaaa aaaaatacac
     2761 tgtcacgtac tgatgccacg gaagagctga cacgtctttc cctggcccgt gcagagggat
     2821 tcgataaggt tgagatcacc ggcccccgcc tggatatgga taacgatttc aagacctggg
     2881 tggggatcat tcattccttt gcccgccata acgtaattgg tgacaaagtt gaactgcctt
     2941 ttgtcgagtt tgcaaaactg tgtggtatac cttcaagcca gtcatcccgc aggctgcgtg
     3001 agcgcatcag tccttccctg aagcgcattg ccggtaccgt gatttcgttt tcccgtaccg
     3061 atgagaagca cacccgggaa tatatcaccc atctggtaca gtcagcctac tacgatactg
     3121 agcgggatat tgtgcagtta caggccgatc cccgcctgtt tgaactgtac cagtttgaca
     3181 gaaaagtcct tctccagctt aaggcgatta atgctctgaa gcgacgggag tccgcccagg
     3241 cactctacac ctttatagag agcctgcccc gggatccggc accgatatcg ctggcgcggc
     3301 tgcgtgcacg cctcaatctg aagtctcctg tattttccca gaaccagacg gtcagacggg
     3361 caatggagca gttgcgcgag attggatatc ttgattacac ggagatccag cgggggcgga
     3421 caaaattctt ctgtattcac taccggcgtc cccggttaaa agcgccgaat gatgagagta
     3481 aggaaaatcc gttgccacct tcacctgcgg aaaaagtcag tccggagatg gcggagaagc
     3541 ttgccctgct tgagaaactg ggcatcacgc tggatgacct ggaaaaactc ttcaaatccc
     3601 gctgaatata aactgtagtc agtgaagagc gctcctttac tgactacagc ttatattaac
     3661 tggtgcagtg agtggtctgc tcactgcagt ttatattctt gttccctgca gtgctgtttg
     3721 tagctgagct gccatctgcc tgtcccttac gtaagtgatc ccgtaacctg atgctgaggc
     3781 attgctccct tcataaaaca tgacttactc actacagctt atatacatgc tccagcttat
     3841 gttatgtctg ttctgctgac cacagcttat ataaggaagc gcgtatgccg tcacctcagg
     3901 ggaggcagtg tacgcaggat ctccgcagca tcccgcccgt caccggtgaa aggcactgcc
     3961 agcgtggcag ccatatccag cgcaaacact ctggtataaa cctccatcga acgtggatcc
     4021 ctgtgaccga ccagtgcctg gatgactttc cggggctggc ggtgatagag catgtgcatg
     4081 atatagctgt gccggaaggt gtgtggtgtg accggaatcg aaaagtgaac cccgtctgct
     4141 tcggcccgtc tgacagcctg cttcagccag ttgcgcatgg tttcgtcggt cacggcccat
     4201 aatggttcac gacgccgggg ccgggtggtg atcatccagc tttccatctg cctgacatag
     4261 cttatatctg tcagcggaac caggcggact tcatcttttg gcgggcgtcc gcgtcgcgca
     4321 cgcacttttt cggacaggat ccgcacaaac ggtcgcagac catccagatc aaatgattcc
     4381 ggcgtcagca tccgggcttc gccaatacgc attccggtat tccagagggt ggcaaacagc
     4441 atatggtgac gctgatccgg catatagaag agaagggcac tgacttccgg agccagcagg
     4501 taggccggtg tggcccccgt ggaagtggcc attcttctca gggagagcgc tgttgcaaaa
     4561 tccacccccg gcgcaatggg taacagggag acgcgttctg gtgaattctg caggggaatg
     4621 acattgttca tgagttattt ttcacgtagc acacgcactg tttatggata caggtattct
     4681 gacctgaacg cccggatcct tccacgacaa taaatcgcag cgcgcagatc cttttgtttg
     4741 cttatcccac attatcgcgg gtaaaaccaa attggaaaag gattcttttt ttgctcagat
     4801 ccttcatgaa gccagtaatg aagcgggtgt agggctgttt tatcgttttt ggggtaatac
     4861 caggacgcta atgatctatc aatggatgga tgtaagtgac aattgaaaac tgaaaaatgc
     4921 gctatgtaga ttctgacaga tttatgcacc tgaaatacgt tcaaaatcca catagcgtaa
     4981 ctctgatttt ccttaccaga aaa
//
LOCUS       DAZGAP010000273         4931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGAP010000273 DAZGAP010000000
VERSION     DAZGAP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.274"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1173
                     /gene="ppk1"
                     /locus_tag="ACHLAM_004885"
     CDS             <1..1173
                     /gene="ppk1"
                     /locus_tag="ACHLAM_004885"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO7856276.1"
                     /translation="DFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFDAIRERDVL
                     LYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVVV
                     ELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVVRYAHIGTG
                     NFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQNSRRLLYE
                     MVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGMCSLIPNLE
                     GISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEVATPLLDPR
                     LKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIKSLEQPE"
     gene            1178..2719
                     /gene="ppx"
                     /locus_tag="ACHLAM_004886"
     CDS             1178..2719
                     /gene="ppx"
                     /locus_tag="ACHLAM_004886"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO7856277.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(2758..>4931)
                     /gene="pdeF"
                     /locus_tag="ACHLAM_004887"
     CDS             complement(2758..>4931)
                     /gene="pdeF"
                     /locus_tag="ACHLAM_004887"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO7856278.1"
                     /translation="ILPIFAHINTFAHISSGEVFLFYLPLALMISMMMFFSWAALPGI
                     ALGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFTPRRNNVSHGDSRLISQRLF
                     WQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVPLCY
                     FIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAIWLLALGALLLLLCMPLNEKSTIFS
                     TNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLTITSS
                     SYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSALCY
                     LRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQE
                     RITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNA
                     PENMQRRGAMYLQRELKDKVAMMNRLQRALEHNHFFLMAQPITGMRGDVYHEILLRMK
                     GENDELISPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSV
                     CQARFPVEISQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDF
                     GTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVE
                     NEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         1329 a         1211 c         1211 g         1180 t
ORIGIN      
        1 gactttatta atttccccaa tgtcggcaaa gccaatctgg tgaacaaacc actgccgcgt
       61 ttacgccaca tttggtttga taaagcccag ttccgcaatg gttttgatgc tattcgcgaa
      121 cgcgatgtgt tgctctatta tccttatcat acctttgagc atgtactgga actgctgcgt
      181 caggcttcgt tcgacccgag cgtactggcg attaaaatta acatttaccg cgtggcgaaa
      241 gattcacgca tcatcgactc gatgatccac gccgcacaca acggtaagaa agtcaccgtg
      301 gtggttgagt tacaggcgcg tttcgacgaa gaagccaaca ttcactgggc gaagcgcctg
      361 accgaagcag gcgtgcacgt tatcttctct gcgccggggc tgaaaattca cgccaaactg
      421 ttcctgattt cacgtaaaga aaacggtgaa gtggtgcgtt acgcacacat cgggaccggg
      481 aactttaacg aaaaaaccgc gcgtctttat actgactatt cgttgctgac cgccgatgcg
      541 cgcataacca acgaagtacg gcgggtgttc aactttattg aaaacccata ccgtccggtg
      601 acatttgatt atttaatggt gtcgccgcaa aactcccgcc gcctgttgta tgaaatggtg
      661 gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagcg gtatcaccct gaagctaaat
      721 aaccttgtcg ataaaggcct ggttgatcgt ctgtatgcgg cctccagctc cggcgtaccg
      781 gttaatctgc tggttcgcgg catgtgttcg ctgatcccca atctggaagg cattagcgac
      841 aacattcgtg ccatcagtat tgttgaccgt taccttgaac atgaccgggt ttatattttt
      901 gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg actggatgac gcgcaatatt
      961 gattatcgta ttgaagtggc gacaccgctg ctcgatccgc gcctgaagca gcgggtgctg
     1021 gacatcatcg acatattgtt cagcgatacg gtcaaagcac gttatatcga taaagaactc
     1081 agtaatcgct acgttccccg cggcaatcgc cgtaaagtac gggcgcagtt ggcgatttac
     1141 gactacatca aatcactcga acaacctgaa taaccctatg ccaatacacg ataaatcccc
     1201 tcgtccgcag gagtttgctg cggtcgatct tggttcaaac agttttcaca tggtcatagc
     1261 ccgtgtggta gatggtgcca tgcagattat tggccgcctg aaacagcgag tgcatctggc
     1321 ggacggcctg gggccagata atatgttgag tgaagaggca atgacgcgcg gtttaaactg
     1381 tctgtcgctg tttgccgaac ggctgcaagg gttttctcct gccagcgtct gtatagttgg
     1441 cacccataca ctgcgtcagg cgctgaacgc cactgacttt ctgaaacgcg cggaaaaggt
     1501 cattccctac ccgattgaaa ttatttccgg taatgaagaa gcccgtctga tttttatggg
     1561 cgtggaacat acccaaccgg agaaaggtcg caaactggtt attgatattg gcggcggatc
     1621 tacggaactg gtgattggtg aaaatttcga accgattctc gttgaaagcc gccggatggg
     1681 ttgtgtcagc tttgcccagc tttatttccc tggcggggtc atcaataaag agaattttca
     1741 gcgcgctcgc atggcggcag cacaaaaact ggaaacttta acctggcaat ttcgaattca
     1801 gggctggaat gttgcaatgg gcgcttccgg tacaataaaa gccgcccatg aagtgttaat
     1861 ggaaatgggc gagaaagacg ggataattac cccggaacgt ctggaaaaac tggtaaaaga
     1921 agttttacgg caccgtaatt tcgcatcgct gagcttacca ggtctttccg aagaacggaa
     1981 aacagtcttc gttcctggac tggcgatttt atgcggtgtg tttgatgctt tagccatccg
     2041 tgaactgcgc ctttctgacg gggcgttacg cgaaggcgta ctgtatgaaa tggaaggacg
     2101 tttccgtcat caggatgtgc gtagtcgcac cgccagcagc ctcgccaacc agtatcacat
     2161 cgacagcgaa caggcccgac gggtgctgga taccactatg caaatgtacg aacagtggcg
     2221 ggaacagcaa ccgaagctgg cgcatccgca actggaagcg ctactgcgat gggccgccat
     2281 gctgcatgag gtcgggttga atatcaacca cagcggtttg catcgccact ccgcttatat
     2341 tctgcaaaac agcgacttgc cgggttttaa tcaggaacag cagttgatga tggcgacgct
     2401 ggtgcgctat caccgtaaag cgattaagct cgacgatctg ccgcgcttta ccttgtttaa
     2461 gaagaaacag ttcctgccac tgattcagct attgcgcctt ggcgtattac tcaataatca
     2521 acgtcaggca accaccacac cgccaacatt gacgctgatt accgatgaca gtcactggac
     2581 actgcgtttt ccgcatgact ggtttagtca gaatgcgctg gtactgcttg atctggaaaa
     2641 ggagcaagaa tactgggaag gcgtggctgg ctggcggttg aaaattgaag aagaaagtac
     2701 accagaaatc gccgcttaat gctttgcggg ccgacgagaa atgtcggccc gcattattca
     2761 ggcactttcg cgaatgggtt cgatttcatt cagcgtatca attaacggtt gcggcttacc
     2821 aataagataa ccctgcatat aatcgatccc caaagagagc accgcctcgc ggatctcttc
     2881 gttttcaacg tactctgcca ctacccgcat tttcttcatt cgcgccaggt ggcaaatcga
     2941 cgccactatc tgataatcca gactatttga cacaatattg cggataaaac tgccgtcaat
     3001 tttaagcaga tcggcattca catttttaag ccgcgcatag ctggcgtagc cggtgccgaa
     3061 atcatcaatc gcaatctggc agcctaattc ctgaagatgc tgcaaggtga tttgcgcctg
     3121 cttaacattg gtcagagcat tactttcggt gacttcaaaa ataagttgcc acgcttcaat
     3181 ctgatattta gccagcagct gactgatttc aacaggaaaa cgagcctgac ataccgaggt
     3241 tggcgacaga ttaatcgcaa aacggtgagc gggcatcttc gctctgtttt cagccataaa
     3301 ttgcagcgta tgctcaatga cccacatgtc gatactcgac gataaaccaa attcgtgcgc
     3361 gaccggtaag aagctatcgg ggctgatcag ttcatcattc tcacctttca tgcgcagaag
     3421 aatttcatgg tagacatcac cacgcatacc ggtaatcggc tgggccatca ggaaaaaatg
     3481 gttgtgttcc agcgcccgct gtagccgatt catcatcgcg actttatctt tcaattcgcg
     3541 ttgcaaatac attgctccgc gacgctgcat attttccggg gcgttggtca cgatagaaag
     3601 ttcggccact gtatttagct ctcccagcag caggtagata tgattcactg gcgagcgcac
     3661 atagcagtaa ctgacgccaa tctgcggttg catcggcatt ccatcccaaa agaaacgaaa
     3721 ttgcttgaga tggctatcca gtgcggtaat gcgctcctgg tgcgattctg tattcagccg
     3781 cagcgcgaga tcgttacccg aaagctgata aacatcttca cccggttcca gcaatggtga
     3841 cagccagtga gaaagttttt gcttgtattg aatccgcagc atgatgccat aattcttaac
     3901 cagcatttcc atgccaggga tgcgtaaata acaaagcgca gaccaggggg catcacgtaa
     3961 cgcgcgattc agggcgcgaa catttggcag atgaaccacc gggtccacat acgcaagccg
     4021 ctgaatgcgt ctgactaccg ctcgctgacg ggttgccagt acagccatat aattgacaat
     4081 aaaagagaat accagataac tggaggaggt tatggtcagc tgcgtggtat agccaggata
     4141 aatgggaatg taattttgat agctgtggat gctgatcatc aacacgaccg cccagagcaa
     4201 tgaaatcagc ttataaccat agcgcatcgc tccccacatc atcaggggca gcaataatga
     4261 caaggtgtaa ttggtgctaa aaatcgtgct tttttcattt aacggcatgc ataacagcaa
     4321 tagtaaagca cctaatgcca gtagccagat tgcgaactct tttttggtaa ctttggcatc
     4381 aacctgctgt tttaattgcg aataatagct acgtaaataa aacggatttc gcaccacccg
     4441 aatgatgaag tagcacagcg ggacaccaat cagattaccc accagcaagg cctgataatt
     4501 gattaaggtc ccgaggttaa agggcatgac gccgaccaga ttttctctgc tcgccagtaa
     4561 tccaacaaac gcagcaaact ggaaaagtat cagaaacagc gttgcaggaa acacaatctg
     4621 ccagaatagt cgctgggaaa ttaaacggct atcaccatgt gaaacgttgt tacgccgggg
     4681 agtaaaaacc ctgtaaccgc cccaacagag aatgataatg ataataaaat tagccgttaa
     4741 tgagagcgtt tcgtaaaaac ccagctctgc atatttgcgg acaaaaatcc ctaaggcgat
     4801 ccctggcaat gccgcccagc tgaaaaacat catcatgctg atcatcagtg ccagtggcag
     4861 ataaaagaga aaaacttcac cggaagaaat atgcgcgaaa gtattaatgt gggcgaaaat
     4921 gggcaagatt a
//
LOCUS       DAZGAP010000274         4919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGAP010000274 DAZGAP010000000
VERSION     DAZGAP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.275"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..284)
                     /locus_tag="ACHLAM_004888"
     CDS             complement(<1..284)
                     /locus_tag="ACHLAM_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7856279.1"
                     /translation="MTYEVIITSEAEQQIINLHRYITEKAGSAIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGVYHGGQSY"
     gene            complement(290..547)
                     /locus_tag="ACHLAM_004889"
     CDS             complement(290..547)
                     /locus_tag="ACHLAM_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO7856280.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            683..961
                     /locus_tag="ACHLAM_004890"
     CDS             683..961
                     /locus_tag="ACHLAM_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856281.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWLQHPPCTEEQAEW
                     LIQCYRRRGYEFEKALSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            963..2012
                     /locus_tag="ACHLAM_004891"
     CDS             963..2012
                     /locus_tag="ACHLAM_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7856282.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAAWMIDVIRHAMNDTQERE
                     LSLAELCWWAVCHQVADALPESVLRRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            2025..2399
                     /locus_tag="ACHLAM_004892"
     CDS             2025..2399
                     /locus_tag="ACHLAM_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7856283.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKSGERYRRAVALIVRQQ
                     QLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            2396..3217
                     /locus_tag="ACHLAM_004893"
     CDS             2396..3217
                     /locus_tag="ACHLAM_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7856284.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            3445..3642
                     /locus_tag="ACHLAM_004894"
     CDS             3445..3642
                     /locus_tag="ACHLAM_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7856285.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            3793..4845
                     /locus_tag="ACHLAM_004895"
     CDS             3793..4845
                     /locus_tag="ACHLAM_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7856286.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELES
                     ERFLQTVKEIRDDFCR"
BASE COUNT         1245 a         1148 c         1368 g         1158 t
ORIGIN      
        1 tcataacttt gccctccatg gtaaacgcca gcgataaaga ctttattgcc atcaacggca
       61 aaagcaatga tcgttctgtg gcggaagtga gttacccgca ttccctgtcg aatatcatcg
      121 cgtttattgc cccgatgcgg gaatgtggaa aaaccatcaa gataatcaag aagcgcgttg
      181 gcataattgt cagcaatggc gctccctgcc ttctctgtta tatatctgtg caggttgatt
      241 atctgctgtt cggcctcaga agtaatgatg acttcatacg tcatgcagat tacttcccgg
      301 ctcgaatcgc ggcgcgaacc tgtgaaatgg agcgtccgtt gtttgggttt tcgcggatag
      361 aatcgagaga tggggcggct gaatgcgtta accacgcttc gattgcttta tcgcgctcat
      421 tcagtgcgcg aagcccttca cgaatgacct cactttctga agcataggca ccggaagcca
      481 cacgggcgcg caccatgtca gccatctcgt tagttaatgt aatgctgaat tgttgggttg
      541 tacgcatggt aaacctcacg gagtaggata gaacactatt cgatgatagc acactgcctg
      601 ttgacgacac cagaaatcag atacaatatt gccgcacgcc agcctgaaca actggcacct
      661 gctgcgccag cagagacaac cgatggcgca cgataccaga ttttacaatt cggataactc
      721 cgccgcccct gccagcaggc acgggcggcg ttctcatgca ttcaaatctg actggttaca
      781 gcatccccca tgcactgaag aacaggccga atggctgatc cagtgttacc gcaggcgcgg
      841 atatgagttt gagaaagccc tcagcttcga tcgtcgtcac tggataatct ccgtcaggct
      901 cccttattcc gaacgcccac cgcgtccgtc ccgcacattc cagcagcgca tctggaggta
      961 acgtgcgggt attacttcga cctgttctgg taccggaact cgggatggtg ctccttaagc
     1021 cgggccgtga atcaatgtca gcattccata acggcagaat attggtggaa ccggaaccga
     1081 aaaacatgcg cggtctgccg tccggagtcg ttcctgccgt tcgacagccg ctggcagagg
     1141 ataaaacatt actgccattt ttcagcaacg aacgggtgat tcgtgctgcg ggtggcgctg
     1201 gtgcactgtc tgactggtta ttacgtcacg tgaaatcctg ccagtggcca cacggtgatt
     1261 atcatcacag cgaaaccgtc attcaccgtt acggtaccgg cgcgatggtg ttgtgctggc
     1321 actgtgacaa ccagctgcgc gaccagacat cagaatcact cgatcaactt gctcagcaga
     1381 atctggcagc ctggatgatt gatgtcatcc gtcacgcaat gaatgacaca caggagcggg
     1441 aactgtcgct ggcagagtta tgctggtggg ctgtttgtca tcaggtggct gatgcgcttc
     1501 cggagtctgt attgcgtcgt tcgctgggat taccagcgga aaaaatccgc tccgtatacc
     1561 gtgagagtga catcgtaccg ggagaacaga cagccatcag catactgaag cagcgcacaa
     1621 aaaatattgc gctgccactt cacgtccacc agcaacaaaa tccaccacag aaaaaaacgg
     1681 ttgtcagtat cgctgttgat ccggagtctc ctgaatcgtt catgaggcgg cctaaacgtc
     1741 gccgttgggt taatgagaaa tacacgcgct gggtaaagac acagccgtgt gcgtgttgtg
     1801 gtaagcctgc tgacgatccg catcacctga ttggtcatgg tcagggggga atggggacaa
     1861 aggcccacga tattttcacg ctaccgttgt gccgggagca ccacaacgaa cttcatgcag
     1921 acccgctgaa gtttgagaaa aagtacggct ctcagattga gttaattttt cgttttcttg
     1981 atcacgcctt tgcgactggc gtgctcgggt aaaagaggtg actgatgctc atagatttgg
     2041 ttttacctta cccgccgacg gtgaacactt actggcgacg ccgtggcagc acatattttg
     2101 tatcaaaatc cggggagcgt tatcgccggg cagtggcact tattgttcgc cagcagcagt
     2161 tgaaattaag cctgtccgga cggctggcaa taaaaattat tgcagagcca ccggataagc
     2221 gtcgtcgtga cctggacaat attctgaaag caccgctgga tgcgctgacg cacgcggggt
     2281 tgttaatgga cgatgagcag tttgatgaaa tcaatattgt acgtggccag ccagtatctg
     2341 gtggacgtct gggggtgaag atttacccca taatgcttga agggcaggtc aaaaaatgaa
     2401 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     2461 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     2521 gaatcgggct gagatggggt tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     2581 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     2641 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     2701 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     2761 aaaaaaattt attgaaagcg aggtttttac aaacaagatc cagtatccgg atggtaagcc
     2821 gccagtgtgg gcaaagtgca caaaaggcgt gtatccgtct tactgggagg aatggaaaaa
     2881 agtcagggag gtggtaaaag ttgcctgtcc ggagtgtgga gggaagggag aggtatccac
     2941 tgcctgtaaa gattgtcgtg ggcgcggtgt tgccattcat cgtgaagagt cggaaaaaca
     3001 gggtgtgccg gttttcagaa actgtcagcg ttgtggtggg cgtggctatg aaagattacc
     3061 ttcaacggag gcatttaatg ccatatgtaa tgtaaccgat gccatatctc tcgatacatg
     3121 gaaaaaaaca gttaaacgtt tttacgatac gctggtggtg cagtttgata ttgaagaagc
     3181 atgggcagaa caacaactga aaaaggtgac cagatagctt tgttgatttt tcccgaatct
     3241 gtggtaaatt tgccctaacg atgggtgttt tatgcctgac gttagaagat tttttacacc
     3301 ccgccgcctg gcgggttttt ttatgactga aatcacgcca gtacagtaaa cgcgctggtg
     3361 gcggtgaata ccggtctttc agcttgctgg ctttttcgac aagagttatt ggtgtgtcac
     3421 gttaaccgga aaagggaaaa agacatgctg aaacagcagg atatgaccga aaccgccaga
     3481 gtggtgttta atgaattaag cgtcaccgaa ccggcgactg tcggggagat tgcacagaat
     3541 acttaccttt cacgtgaacg ctgccagtta atactgaccc agctggttat ggcggggctg
     3601 gcagactatc agttcggttg ttacagacgc cttcagtcct gaaggctttt ttatttgtgg
     3661 taaatgggcg gctggtgggt gttaggggca ctcaccagcc atctgctcat gcgtccggat
     3721 cacaagcaaa cctcaggccc actgctttgc gcaaaagcag aatgagccta tcagagacag
     3781 gcttaatgat ccatgcttag tactgtaaaa atatccagtt gtgagttaat caacgccgac
     3841 tgcctggaat ttatccggtc gttacccgaa aattctgttg acctgatagt cacggacccg
     3901 ccgtacttta aagtgaagcc tgagggctgg gataaccagt ggaagggcga cgatgattac
     3961 ctgaagtggc tggaccagtg tcttgcgcag ttctggcggg tgctgaaacc tgccggaagt
     4021 ctttacctgt tctgtggcca tcgcctggca tctgacattg aaatcatgat gcgtgaacgc
     4081 ttcagtgtgc tgaaccatat tatctgggcg aagccgtccg gacgctggaa cgggtgcaac
     4141 aaggaaagcc tgagggcgta tttccccgcc acagagcgca ttctgttcgc ggaacattat
     4201 caggggccgt atcgtccgaa agatgccggg tatgaggcga agggcagggc actgaaacag
     4261 catgtgatgg ccccgctgat tgcttacttt cgtgatgcac gtgctgccct ggggataacg
     4321 gcaaaacaga ttgctgatgc cacaggaaag aaaaacatgg tgtcgcactg gttcagtgcc
     4381 agtcagtggc agctaccgga cgaaagcgat tatctgaaat tacaggcgct gtttgcccgg
     4441 gtggcagaag agaagcatca gcgcggagaa ctggaaaagc cacatcacca actggtcagc
     4501 acatacagtg aactgaaccg acagtatacg gaactgcaga gtgaatataa gcatctgcgg
     4561 cggtatttcg gtgtgacggc gcaggtgccg tacaccgatg tgtggacgca taaaccggtg
     4621 cagtactatc ccgggaaaca tccgtgcgaa aaaccggcag aaatgctgca gcagataatc
     4681 agcgcgagca gtcgtccggg tgacttggtt gcagatttct tcatggggtc gggctcaacc
     4741 gtcaaagcag cgctggcact ggggcgtcgt gcaactggtg ttgagctgga gagtgaacgt
     4801 tttttacaga cagtgaagga gatccgggac gatttttgca ggtgacagag gttgaggaaa
     4861 ggttgaaggg aggatcattt gttattgttg tatataatta ttctacccct ttcctgagt
//
LOCUS       DAZGAP010000275         4901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGAP010000275 DAZGAP010000000
VERSION     DAZGAP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.276"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..78)
                     /locus_tag="ACHLAM_004896"
     CDS             complement(<1..78)
                     /locus_tag="ACHLAM_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFO7856287.1"
                     /translation="MLCCILISIISEGDMKIFISLFLFII"
     gene            complement(44..607)
                     /locus_tag="ACHLAM_004897"
     CDS             complement(44..607)
                     /locus_tag="ACHLAM_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7856288.1"
                     /translation="MNLIFSKYSVFFILFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(607..3201)
                     /locus_tag="ACHLAM_004898"
     CDS             complement(607..3201)
                     /locus_tag="ACHLAM_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO7856289.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELNNPV
                     SYATLECR"
     gene            complement(3238..3990)
                     /locus_tag="ACHLAM_004899"
     CDS             complement(3238..3990)
                     /locus_tag="ACHLAM_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7856290.1"
                     /translation="MKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNTFVPPKSDI
                     TVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(4091..4795)
                     /locus_tag="ACHLAM_004900"
     CDS             complement(4091..4795)
                     /locus_tag="ACHLAM_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7856291.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT         1441 a         1113 c          909 g         1438 t
ORIGIN      
        1 tattataaac aaaaataaac taatgaatat cttcatatct ccctcactga taatcgatat
       61 caaaatacaa cagagcattt gcttgcccgg catgtacatt ttcctgagtt gatttatagc
      121 gaagtttaaa cgtcaaatta gcatcaccgt tttcatttaa cactaaatca cgaaaacctg
      181 attcaccatt aatagcaatc gccacatcat tgttatccaa tatttctatt cccagtccag
      241 tcgcactatc tgcaccggcg gagagacgca gtaatgcgct attaatggca tctgcatcac
      301 cagtaaaccg cacattggcg ctcacaccag aactataaac agtacttttt gcagtcgggc
      361 atccttgtac attaatcgta aacgtttgac tcggagaaac atcacctaca tttaaaaatt
      421 cattagcatc ataagtgccc atttttaccg actgatcttt acttccggta gtaatgtcac
      481 agctttttgc caacaaaatc ccagtaaatg taacaacaac tgaatcattg gcctgccctg
      541 agagcggaaa agcaaaaaaa gcgaaaagaa taaaaaatac agaatattta gagaatatta
      601 aattcattag cggcactcca gagtagcata gctaacagga ttattcagtt ctgtttcagg
      661 gagcttgtaa ttagcctgac atttttgctg aacaccatct ccccactgga tattaataac
      721 accttctaaa ggcaatcctg agagataaag ttgtccatta tcgccgacaa tcccggtaat
      781 ttgactacta tttttaatct gtgcgctggc tgtagcaccg aatgggactg atttattacg
      841 ataacgaact gtgaacaaac cacgaatacc gatatgagca gcaaactctg cacgaactaa
      901 cgcacctttt cccggaataa cttcagtgga agtgatatcc agttcagtat gcatattcat
      961 tgacatagga tctaatgaaa tattattacg tctatattct gttgcataag gaacaactgc
     1021 atatccacgc cagtcagtgg atattcctgt cccccgctgg acatccacat tattcgctcc
     1081 aggcgctttt atcaaaacat tagtttcacc taatggttga ctcaacgtaa gaccattttc
     1141 atgtacaaca agagcaccac tgatgccata gtttatctgt tgattacctt cattatcgta
     1201 actgtaggcg gaattcaagg aaccatatgc cccacgataa ttaacaccaa cagaaccact
     1261 actgctatta tcctgattag caaaaccatg ttgtatgtta taactgagat tatgctgctc
     1321 caacagagaa ccagataccc ccgtatattg gtttacctgt ccgtggttat cctgactata
     1381 accatacgtt gcagtcatgg cattcgatga tgagcgccca tcattcgtgg aagataacca
     1441 attacttata ggtaatgaga catttagcga gacaatttta tccgcatttg tccctggtga
     1501 tttactgtaa ttccacgcaa catttagata gataccctta atactggtgt tataaccaac
     1561 ttggtataaa gaatcttttt tatcagtatt ccagtatgtt tgctgactag ccgataaata
     1621 catagacccg tagttaccga aaggttgcga tacactagcc tgaaattttc cacgtttgct
     1681 ataacgcaag ttataaaaat cattccagtt ataaacgtaa tctttatcat ctaacgtttt
     1741 tggatcaaca acagtccctg acatttgttg gtatgtcgta tcacttaaag tgtaaaagcc
     1801 ctgggttgaa taacgatagc caataatatg gaatgctgtt cctgtctgaa ctaacgattt
     1861 ggaataaaga aaacgataag attgcccttg atgtttgctc ccatccacaa gcgaactgtt
     1921 agcctgagta acatcaaacg atgttgcacc aaatacaccc agattcaggc caaggccgag
     1981 cgccaacgcc cggtaatcct cagagaattg tgccccacca taagccgtaa tatcccaggg
     2041 taaaccccaa attaattccc cctgaaatac ataaggactt ttctggttat agctattgct
     2101 tcgatattgg ccaaccgtag tagaatattt tgaatgacct tctcgttgca ggatggggac
     2161 agcggcataa ggtacggtat agacagtttc tgaattatca gactctttga tggtaacttc
     2221 cagatctccc gcagatgatg tcgggttaag atcgctaatc tcaaacggtc ccggcggcat
     2281 ataggtttga taaatgacat aaccattctg tttgattgtt acctgagcgc gacttttcgc
     2341 aattccgcgc accgtgggag cgaaaccact ttgactatcg ggcaacatat tttcgtcaga
     2401 ttccagtttt atgccacgaa tactaacact gtcgaaaaca tctgatgatg accatgtatc
     2461 tcctagcgtt aattcacttc tgaaaggaat aataacgcgc tgcaacgtac tactgatatg
     2521 ctccagttta cctgctgaat tactcccacg actccaggtt gaataatcgc gaaaacgcca
     2581 tgcaccaata ttgatcccag cgcgaagatt taaataatag ttatttgaag agtaatcacc
     2641 acgatcgtta ttaccagaaa agtcataatt cagcaaaaaa gctgtaatac cgttatccca
     2701 ctgttctggt ggaatatatc cgcgggcgtc acgttttatt atcgcctgcg gaaaggtcag
     2761 tttaagtctc tgtgcatcca gctccggcat gtaatgcaca tcaggaaacc agatacgcaa
     2821 atcaaggcaa ctattttcag ataaagccaa agcctgctct ggcagcgcat tcttgttaat
     2881 tccaaggctt aataagtcag caagtgaaag gcagggaatc agttgattct catcgtctgc
     2941 tttaaaagtt acagtacgag agagtaaagg ggaatcattg acccaaactt cgacttcata
     3001 gctaccagga agataagtca tccctttctc gaaacgggat aagtcagcaa cagcttcagt
     3061 atcacccgac agaaaagcag ggttaaagcg cattcccgcg attgaacatg caggcataat
     3121 aaccagaaga agcggtacaa cccgtttcat ctgcttttgt gccgccatcc aatattcgca
     3181 tcttttgagc tttaggttca tatccctgac ctgtgttgca tctgaactat cccgttgtta
     3241 ctgcattatt cctttctgcc gctccgtcgt agcgccaaag tcattaattg tttgataagt
     3301 aatttcacca gccaacgtca ctgccgacga gaccgtaata tcgcttttcg gtggtacaaa
     3361 cgtattgggc aatttactcc cttgtacttt aattcctgtc agtgtcaggt aataaggcga
     3421 aggattgata acttttagtt gtttgccctc atacttaaac cgcaacatat ttcgtgcttt
     3481 tcctggttgc acaggtagat tttccgggcg ataaaatagc ttgattttgt tttgtaatgc
     3541 caactgtaag gtattcttct gtgtattttt ctcatctgtt ggcggaattg ctttaacatt
     3601 catccaatac aaagtttcac gatcggtcgg caaattagtt ttaccaagaa acatgatacg
     3661 taatgaagct tctttacgcg ttggcaacat aaagagggga ggagtgataa caaaatcctg
     3721 gctctttttt tccgttttac tgtcatcaac ccatgattga attaaataac tggtgttgtc
     3781 accgctattt tgcacactga gtgttatttg cttactttct gccggataaa tcaatcgcgt
     3841 tacgccaagg ccaaacccgc ccgcggacgc attattaata aataatatcg atagtaaaaa
     3901 aagaatccct gagcgtataa tattttgtga tacttttaat tcatccctga atccataaac
     3961 ccatttttta tttccaataa tacatttcat acagcatcct tgttgatgaa ataacacctg
     4021 ttattcatac tctattaaat aatttcaatc tcaggttaca actttttgta acctgaaatc
     4081 gtatttatat ttactcgtac agcaccacaa aacttgctgt tgacttaact acaccaggtg
     4141 caacagtgct accgttttga gcataacctg cagtaaaggc cgcgctgaaa tcatgggtcg
     4201 tagcatttcc tggaattgta taagcggcgg tggaggcgct accctctgcc gcagtagtat
     4261 tcaggttaat cagaccatta ttcgtcgtac ctttggagta aattcgaatc cctacaccag
     4321 tcgcccccgc attgccgctg ttgtcagcat ccgttgatcc ttctacttcg ttagcgagcg
     4381 ttttattagc gctatctgcg gtaccggtaa agcggaactc aattttctga tcctctgtat
     4441 tgctacattc ggtcagatta atattgaaat cggtctggtt gctcgccccc tgtgcttcta
     4501 tgtcgatacc agtgaaaagg ttggaaggca caaccggcag gacaacttca atctgttttg
     4561 aatcggttgc gaccttacag gtagctttca ctacacggcc agtaaagcgg attttccccc
     4621 ccattgaatc aacgttgtca tccatatcag tgagcctggg ataatcaacg gtgctccagt
     4681 cagtatccgc tccaccaaat tgtgctgcat cgccgggtgc tgcggttacc gcagtagaca
     4741 tcagcatacc tgatgccagt aatactgcgg ataaattaga taaattgaat ttcatttata
     4801 cctttccttt tgttaaaaaa tccaagcaac attggtgttt ttacattctg cgttaataag
     4861 ttatatctca caaaaaacaa gctgacgctg gctaattcca g
//
LOCUS       DAZGAP010000276         4886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGAP010000276 DAZGAP010000000
VERSION     DAZGAP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.278"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..148
                     /locus_tag="ACHLAM_004901"
     CDS             <1..148
                     /locus_tag="ACHLAM_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856292.1"
                     /translation="AVINARRREMGQKPLTTAQIVDEICDFVANQQAVFLGGHYILQG
                     SRNR"
     gene            1186..1416
                     /locus_tag="ACHLAM_004902"
     CDS             1186..1416
                     /locus_tag="ACHLAM_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856293.1"
                     /translation="MTVSIVSPSAAAVKPRRHTRFRREDIPAPEIDPVLKAFGRHIAR
                     SFRRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            1468..2829
                     /locus_tag="ACHLAM_004903"
     CDS             1468..2829
                     /locus_tag="ACHLAM_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO7856294.1"
                     /translation="MTLSAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTISQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNSFRLVNVYITDMKITEI
                     PQK"
     gene            2876..3439
                     /locus_tag="ACHLAM_004904"
     CDS             2876..3439
                     /locus_tag="ACHLAM_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO7856295.1"
                     /translation="MHSQLKERIRLMRARLDNAAPLAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRHVAPGAMCDAVEINSGLVRYLRDNFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPCGTFAYTRVPTMIIRLRA"
     gene            3439..3666
                     /locus_tag="ACHLAM_004905"
     CDS             3439..3666
                     /locus_tag="ACHLAM_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856296.1"
                     /translation="MTQGRREDKCPSARAGKRPRAGGAERGRADAPPLSLRGRSDGGF
                     CFFMPVICLLLMLLFLLLLLLLLLFFFFLLL"
     gene            3591..3836
                     /locus_tag="ACHLAM_004906"
     CDS             3591..3836
                     /locus_tag="ACHLAM_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856297.1"
                     /translation="MSVTDVVVPATATATATVLLFPAALILTTAVLSRLPGAPSAPGC
                     LAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            4309..4836
                     /locus_tag="ACHLAM_004907"
     CDS             4309..4836
                     /locus_tag="ACHLAM_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7856298.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADATKKSGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT         1073 a         1384 c         1460 g          969 t
ORIGIN      
        1 ggccgtgatt aatgcgcggc gtcgtgaaat ggggcagaaa ccgctcacca ccgcgcagat
       61 tgttgatgaa atctgtgatt ttgtggcgaa tcagcaggcg gttttcctcg gtggtcacta
      121 tatccttcag ggcagcagaa acaggtgata cagggcaggc ggtggaggcc gcctgccagc
      181 ccgcgcgaaa acccctggca ggctggcgcc tgccggaagg gatgcgggcc acgctggcgc
      241 gtcgcccgtt acccttcccg tttgtgatgt gtctgactca ggagtgtttc tttcttatgt
      301 tatggaactg ttcctgatgt tgttgttgtt cctttatctg ctgctctgat tctgactact
      361 gcagtgttgt tctgtctgcc gggcgtacct tcggctcccg gctgcctggc ggcatccggt
      421 cgtcaggccc gactccgaca ggcagctgct ggcgcacccg ccagtctccg ccgaacccgc
      481 tgacgcggtt tcgggctgtc gctacggtcc gatgcgtgaa aacccccggc ttgcagcgac
      541 cggaacggtc gcgccaccct tgtcgcgccg tggcctgcgc tccaggcatc ccggcaaaag
      601 gccgccggga tgtaaggagc aacgccgttt cgccaaccgc gcttcacgtc gttgcagcgg
      661 gtccctgctc attgcctgcc gggtgccaca cacccgtatg agtccctgtc gccgggggca
      721 tagacagcca caacctgccg cactgactgt ccagggtaaa tgaacagccc acaaaatttt
      781 ttacccagaa cctggtaaaa aattttgcgt tctgcccagg acaggtgcgt caggccgtgg
      841 cagtgatgcc ccttgcggcg aaggttctca aacgggtgaa gttcatacca cagggaatac
      901 agcagcaggt tcccgcgaaa cgtcaaaacc gcggctcccg aaactcaacg ggtgatgttc
      961 tttaaaaatc aggatttatt taaggctccg gcaatacgca tttattcgct ctgtcagcat
     1021 ccggctggca ggtaatgaga acgcaggaac agaaggagga atacaccgga gaacgtcaga
     1081 cgcagatatg aaaaaagcag gtcagggacc tgcttaatcc ataccgcaga gactgcgcga
     1141 acagtcttta cggcttcatt aattaacgtc ataagaggct aaattatgac agtaagtatt
     1201 gtttcacctt ccgcagcggc tgtaaagccc cgtcgtcata cccgtttcag acgcgaagac
     1261 atccccgcgc cggaaattga ccccgtgctg aaagccttcg ggcgacacat agcccggagt
     1321 tttcgccggg ggcgtggtgt acacatcccc gccatgaaaa acacggcctt cgggcaggtg
     1381 ctccgcacac tcgaactgaa acgcgctttt aactgatgag gtgagccgcc ccgcgagggg
     1441 cggcaacaga cggaaggaga acattttatg acattatcag caatcaccat gaccgccccg
     1501 gaagccgcca gcccggtgca gatgtaccgc gcgacctact caccggatga taacaaactt
     1561 cgcctgtatg ccgtgtcacg cctcgacccg gagacgtata aaaaagtgca tgatgccggt
     1621 ttccgctggg cacccaaaca ggcgctgttt gtggctccag cctggacacc gggccgggaa
     1681 gacgtgctcc tctcacttgc cggagagatt gaggatgaag acagcacgct tgctgaacgt
     1741 caggaagcac gggcggagcg gtttaccgga tacagcggaa agcgggccag tgaatccgca
     1801 caggcacttg atgaagtgga acgactggcc gcgatgatcc cgcccggtca gcccattctt
     1861 gtggggcatc acagcgaacg ccgcgcccgt cgtgatgcgc agcgtattga aaacggcatg
     1921 aaacgcgccg tgatgctctt tgaacgtgcg gaatactggg aagaacgggc gcggtcggca
     1981 ctgcttcacg cgaagtataa agaacgtccg gacgttcgct ggcgtcgtat caaaaaaatc
     2041 gaagctgatt tgcgcaaggc tgaaaagacc atctcacagt cgcagaaata tctgacgatg
     2101 tggcgggctg aatcgctgga tctgaatatg gcaaaactca tcagcagtca tgaccatatc
     2161 agcgcctgtt tcccgctgga tacgtatccg cgcccggcag aaaaaagcca gtatgaagga
     2221 agtcgatcgt tatggtcggc cctggatgat gacatcatca ccacggagca ggcccgcgaa
     2281 attgcgatcc gctgtcatga acggcagatc cagcgtcaac aacgctgggt taaccactat
     2341 cagaaccgcc tgatttatga gcgtgccatg ctggacgaaa gcggcggcgt ggttacccgg
     2401 acacaggatt ttgagccggg cgggcaggtt ttcagccggg gcgagtggct gaccatcatc
     2461 cgtgtgaaca aaagcaacgg ggcggtgagt tcagtcacaa cgccgaatta cagttttctc
     2521 gggtacagcg gcacgatgaa agtgacgccc gatcgcatta cggactacaa agcaccatcg
     2581 gcagaagagg ctgccgtcgc cagccaggcc gcgaagcgtc cgccggtagt caactatccg
     2641 ggggaaggtt tccgggaaat gacaaaggcg cagtgggccg ccctgccccg ggactgtaag
     2701 gccgtgcgca gtgtggcaga gacagaagac cacggggcat accgctaccg ccgcacaatg
     2761 gacaatagtt tccgcttggt gaatgtgtat atcaccgaca tgaaaatcac ggaaatcccg
     2821 cagaaataag gtatatcccc cggtgaaatc cccgggggat gacggagata atgacatgca
     2881 cagtcagtta aaagaacgta tccggctgat gcgcgcaagg ctggataacg ccgcgccgct
     2941 tgctgaaatc cgggctgaat ctcagctttt tgtgactcct gccccggtct gtgatcgcct
     3001 ggtgacgctg gcggagatca gcaaccgtga tcacattctg gaaccctctg ccggcaccgg
     3061 ggccatactg cgggcgattc gtcatgtcgc gccgggggcc atgtgtgatg cggtggagat
     3121 taacagcggg ctggtccggt atctgcggga taattttaac ggtgtcaggg ttcagtgtgg
     3181 cgacttcatg gaatggcagc cggtgcagta ttacagccgg attatcatga atccgccgtt
     3241 cagtcacggg caggatatcc ggcatatcct gcgggccttt tccctgttgc gtccgggtgg
     3301 cgtgctggtt gccgtctgtc tgaacgggcc gcgccagcag gagaagctgt taccgttttc
     3361 tgacgtccgc gaggagctgc cgtgcggcac gtttgcttat acccgtgttc cgacgatgat
     3421 tattcgtctg cgtgcctgat gacacagggc aggcgggagg ataaatgccc gtctgcccgc
     3481 gccgggaaaa gaccgcgcgc cggtggcgct gaaaggggcc gcgctgacgc gccgcccctt
     3541 tcattacgcg gacgttctga tgggggcttc tgtttcttca tgcctgtcat ttgtctgtta
     3601 ctgatgttgt tgttcctgct actgctactg ctactgctac tgttcttctt tttcctgctg
     3661 ctctgattct gactactgca gtgttgtccc gtctgccggg cgcaccttcg gctcccggct
     3721 gcctggcggc atccggtcgt caggctcgac tccgacaggc agctgctggc gcacctgcca
     3781 gtctccgccg aacccgctgg cgcggtttcg ggctgtcgct acggtccgat gcgtgaaaac
     3841 cccgggtttg cagcgaccgg aacggtcgcg ccacccttgt cgcgccgtgg cctgcgctcc
     3901 tggcatcccg gcaaaaagac gccgggatgt aaggagcaac gccgtttcgc aaaccgcgct
     3961 tcacgtcgtt gcggcgggtg cctgcccgtt gttgtgtccg gtgccataca cctgtttaag
     4021 cccctgtcgc cgggggctga tacagccaca acctgccgca ctgactgtcc agggtaaatg
     4081 aacagcccac aaaatttttt acccaaaacc cggtaaaaaa ttttgcgttc tgcccaggac
     4141 aggtgcgtca ggccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa caggtgatgg
     4201 tgaataccgg agggaacaaa gagcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
     4261 actcaacaga tgatgggggc tgaaaacatc aacagaagga gacacatcat ggcagttcgt
     4321 ggcattaaca aggtcattct cgtgggacgt ctgggaaaag atccggaagt ccgttacatc
     4381 cccaacgggg gcgcagtggc aaacctgcag gtcgccacgt cagaaacctg gcgtgacaaa
     4441 cagacggggg agatgcggga gcagacagaa tggcatcgcg tggtgctgtt cggcaagctc
     4501 gcggaagtag ccggtgaata tctgcgcaag ggcgcgcagg tctatatcga ggggcaactc
     4561 cgaacccgta gctgggaaga taacggtatc acccgttacg tcactgaaat tcttgttaag
     4621 accacgggca ccatgcagat gctggggagt gcaccacagc agaacgctca ggcacaaccg
     4681 cagcctcagc agaatgggca gccacagagt gctgacgcga cgaaaaaaag tggcgcgaaa
     4741 acgaaaggcc gtggacgtaa ggccgcgcag cccgagcctc agcctcagcc gccggagggg
     4801 gaggattacg gattttcaga cgatatcccg ttctgactgg cctgactgtg acaaccgccc
     4861 cgtcctgtgc ggggcatcac cggaga
//
LOCUS       DAZGAP010000277         4850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGAP010000277 DAZGAP010000000
VERSION     DAZGAP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.279"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..1432
                     /gene="garP"
                     /locus_tag="ACHLAM_004908"
     CDS             98..1432
                     /gene="garP"
                     /locus_tag="ACHLAM_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO7856299.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1448..2218
                     /gene="garL"
                     /locus_tag="ACHLAM_004909"
     CDS             1448..2218
                     /gene="garL"
                     /locus_tag="ACHLAM_004909"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO7856300.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2248..3138
                     /gene="garR"
                     /locus_tag="ACHLAM_004910"
     CDS             2248..3138
                     /gene="garR"
                     /locus_tag="ACHLAM_004910"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7856301.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3235..4380
                     /gene="garK"
                     /locus_tag="ACHLAM_004911"
     CDS             3235..4380
                     /gene="garK"
                     /locus_tag="ACHLAM_004911"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO7856302.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4413..4789
                     /gene="rnpB"
                     /locus_tag="ACHLAM_004912"
     ncRNA           4413..4789
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHLAM_004912"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1129 a         1162 c         1398 g         1161 t
ORIGIN      
        1 attcccgagt tcattgtcgt aacggcataa atgaaactca taaataacac cttccccata
       61 ataataaaaa tcagcataag tacccgaggt aaataaaatg atgctggaca ccgttgacga
      121 aaaaaagaaa ggcgtgcata cccgctattt aatattactg attattttta ttgttaccgc
      181 cgttaactac gccgatcgtg caacgctgtc tattgctggt accgaagtgg caaaagagtt
      241 gcagttaagt gcggtttcga tgggttacat cttctctgct tttggctggg cctacttgct
      301 gatgcaaatc cccggcggct ggctgcttga taagtttggt tcgaaaaaag tttacaccta
      361 cagcctcttt ttctggtcgc tattcacctt cctgcaaggc tttgttgata tgttcccgct
      421 ggcctgggca gggatctcca tgttctttat gcgctttatg ctggggttct cggaagcgcc
      481 atcattcccg gcgaacgccc gtattgtcgc cgcctggttc ccgacgaaag aacgtggtac
      541 tgcctccgcc atctttaact cggcgcaata tttctcgctg gcgctctttt cgccgctgct
      601 tggctggctg actttcgcgt ggggctggga gcacgtcttt accgttatgg gggtaattgg
      661 ttttgtgctg acggcgctgt ggatcaagtt gattcataac ccgacagatc acccacgtat
      721 gtctgcggaa gagctgaagt ttatctcgga aaatggcgcg gtggtcgata tggaccacaa
      781 aaagccgggc agtgcggcag caagcggacc caaactgcat tacatcaagc aattgctctc
      841 taaccgcatg atgctgggcg tatttttcgg acaatatttt atcaacacca tcacctggtt
      901 cttcctcacc tggttcccaa tttatctggt gcaggaaaaa ggcatgtcga ttctgaaagt
      961 gggtctggtc gcctcgattc cggcactgtg tggctttgcg ggcggcgtgc tgggaggtgt
     1021 cttctcggat tatctgatca aacgcggttt gtccctgacc ctggcacgta agctaccaat
     1081 tgtgctggga atgttgctgg cttccaccat catcttatgt aactacacca acaacaccac
     1141 gctggtggtc atgctgatgg cgctggcttt ctttggtaaa ggatttggtg cgctgggctg
     1201 gccagtgatt tctgacaccg cgccgaaaga gattgttggc ctctgcggcg gcgtctttaa
     1261 cgtctttggc aatgttgcct ccattgtcac tccactggtg attggctacc tggtaagtga
     1321 actgcactcc ttcaatgcag cactggtttt tgtgggatgt tcagcgctga tggcgatggt
     1381 ctgctacctc ttcgtagtcg gcgacattaa acgtatggaa ttgcagaaat aagcaaaggt
     1441 ataagcgatg aataacgatg ttttcccgaa taaattcaaa gccgcactgg ctgcgaaaca
     1501 ggtacaaatt ggttgctggt cagctctctc taacccgatt agcactgaag ttcttggttt
     1561 ggctgggttt gactggctgg tgctggatgg tgaacatgcg ccaaacgata tttccacgtt
     1621 tattccgcag ttaatggccc tgaaaggcag cgccagcgcg ccagtagtgc gagtgccgac
     1681 caacgagccg gtaattatta agcgtcttct ggatatcggt ttctataact tcctgattcc
     1741 ttttgtagaa acaaaagagg aagcagagca ggcggtggca tcaactcgtt acccaccgga
     1801 aggcattcgc ggcgtctccg tttctcaccg cgccaatatg tttggcaccg tggcggatta
     1861 tttcgctcag tcgaacaaga acatcactat tctggtccag atagaaagtc agcagggcgt
     1921 agataacgtc gatgctattg ccgctaccga aggtgtagac ggcatcttcg tcggccccag
     1981 cgatctggct gcggcattag gccatctcgg caatgcctcg cacccggatg tacaaaaagc
     2041 aattcagcac atttttaacc gtgccagcgc gcacggcaaa cccagcggca tcctcgcacc
     2101 agtcgaggct gatgcgcgtc gttatctgga atggggcgcg acgtttgtgg ccgtcggcag
     2161 cgatctcggc gtcttccgct ctgccactca gaaactggct gataccttta aaaaataacc
     2221 accaccgcaa taagagagat gattgatatg actatgaaag ttggttttat tggccttggg
     2281 attatgggta aaccaatgag taaaaacctt ttgaaagcag gttactcgct ggtggttgct
     2341 gaccgtaacc cagaagctat cgctgacgtg attgctgcag gtgcagaaac agcctctacg
     2401 gctaaagcga tcgctgaaca gtgcgacgta atcataacca tgctgccaaa ctcccctcat
     2461 gtgaaagagg tggcgctggg tgagaatggc attattgaag gcgcgaagcc aggtacggta
     2521 ttgatcgata tgagttctat cgcaccgctg gcaagccgtg aaatcagcga agcgctgaaa
     2581 gcgaaaggca ttgatatgct ggatgctccg gtgagcggcg gtgaaccgaa agccatcgac
     2641 ggtacgctgt cagtgatggt gggcggcgac aaggctattt tcgacaaata ctatgatttg
     2701 atgaaagcga tggcgggttc cgtggtgcat accggggaaa tcggtgcagg taacgtcacc
     2761 aaactggcaa atcaggtcat tgtggcgctg aatattgctg cgatgtcaga agcgttaacg
     2821 ttggcaacca aagcgggcgt taacccggat ctggtgtatc aggcaattcg cggtgggctg
     2881 gcgggcagta ccgtgctgga tgccaaagcg ccgatggtga tggaccgcaa cttcaaaccg
     2941 ggcttccgta ttgatctgca tattaaggat ctggcgaatg cgctggatac ttctcacggc
     3001 gtcggcgcgc aactgccgct cacagctgcg gttatggaga tgatgcaggc actgcgtgca
     3061 gatggtttag gaacggcgga tcatagcgcc ctggcgtgct actacgaaaa actggcgaaa
     3121 gtcgaagtta ctcgctaatg acgtcgtgcc cggatggcgt attgcaatcc gggcctggaa
     3181 tccaggccga ataagagaaa cgccctccgg cgtcatgtgg taacaggcat aggtatgaaa
     3241 atcgtaatcg ccccagactc ttataaagaa agtttatctg ccagcgaggt tgcgcaggcg
     3301 atagaaaaag gatttcggga aatttttcct gatgcacagt atgtttctgt tccggttgcc
     3361 gacggtggtg aaggaacggt ggaagcgatg attgcagcca cccagggggc tgaacgtcac
     3421 gcctgggtta cagggccgct gggcgagaaa gtgaatgcca gttgggggat ctccggcgat
     3481 ggcaaaaccg cgtttatcga aatggcggcg gccagtgggc tggaactggt acctgcggaa
     3541 aaacgcgatc cactcgtgac cacttcacgc ggtactggtg agttaatcct gcaggcgctg
     3601 gagagcggtg cgacaaacat tattatcggc attggcggca gcgccaccaa cgacggcggc
     3661 gcaggcatgg tacaggcgct gggggcgaaa ttatgcgatg ccaacggcaa tgaaattggt
     3721 tttggcggcg gtagtcttaa tactctgaat gatattgata tttccggtct cgatccgcgc
     3781 ttaaaagatt gtgtcatccg cgtcgcttgt gatgtcacca acccgttagt cggcgacagt
     3841 ggtgcttcgc gcatctttgg cccacaaaag ggagccagtg aggcgatgat tgtcgagctg
     3901 gacaataacc tctctcacta tgccgatgtt attaaaaaag cgctgcatgt tgatgtgaaa
     3961 gatgtccccg gcgcaggagc tgcgggcggt atgggcgcgg cgctgatggc gtttcttggt
     4021 gcagaactga aaagtggtat tgaaatcgtc actacggcgc tgaatctgga ggaacatatt
     4081 cacgattgta cgctggtgat cactggtgaa gggcgtattg atagccagag tattcacggc
     4141 aaggtaccga ttggtgtcgc aaacgtggcg aagaagtacc ataaaccggt gattggcatt
     4201 gcgggtagcc tgaccaatga tgttggcgtt gtacatcagc atggcattga tgcggtcttc
     4261 agcgtattga ccagcattgg tacgttggac gaagcattcc gcggggctta tgacaatatc
     4321 tgccgtgctt cacgtaatat cgccgcgaca ctggcgattg gaatgcgcaa cgcggggtga
     4381 caagggcgcg caaaccctct atactgcgcg ccgaagctga ccagacagtc gccgcttcgt
     4441 cgtcgtcctc ttcgggggag acgggcggag gggaggaaag tccgggctcc atagggcagg
     4501 gtgccaggta acgcctgggg gggaaaccca cgaccagtgc aacagagagc aaaccgccga
     4561 tggcccgcgt aagcgggatc aggtaagggt gaaagggtgc ggtaagagcg caccgcgcgg
     4621 ctggtaacag tccgtggcac ggtaaactcc acccggagca aggccaaata ggggttcata
     4681 aggtacggcc cgtactgaac ccgggtaggc tgcttgagcc agtgagcgat tgctggccta
     4741 gatgaatgac tgtccacgac agaacccggc ttatcggtca gtttcacctg atttacgtaa
     4801 aaacccgctt cggcgggttt ttgcttttgg tgcccagggt aagatgaata
//
LOCUS       DAZGAP010000278         4827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGAP010000278 DAZGAP010000000
VERSION     DAZGAP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.280"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..437
                     /locus_tag="ACHLAM_004913"
     CDS             <1..437
                     /locus_tag="ACHLAM_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856303.1"
                     /translation="FLGGRCYTAEKLEKDYLAEVANYSDDRWEAPQRAARLAAAVKRY
                     KTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRSGSQWLVVEVFGEKGRQHRSAD
                     SNPDMVEKMAARYRHAAGQHWSATLAEIERVKRIYQARIKAS"
     gene            1041..1136
                     /gene="dqlB"
                     /locus_tag="ACHLAM_004914"
     CDS             1041..1136
                     /gene="dqlB"
                     /locus_tag="ACHLAM_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO7856304.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
     gene            complement(1503..1802)
                     /locus_tag="ACHLAM_004915"
                     /pseudo
     CDS             complement(1503..1802)
                     /locus_tag="ACHLAM_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2108..2773
                     /locus_tag="ACHLAM_004916"
     CDS             2108..2773
                     /locus_tag="ACHLAM_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFO7856305.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     ILQ"
     gene            2869..3153
                     /locus_tag="ACHLAM_004917"
     CDS             2869..3153
                     /locus_tag="ACHLAM_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO7856306.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFR
                     LPAYFEDILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
     gene            3183..3410
                     /locus_tag="ACHLAM_004918"
     CDS             3183..3410
                     /locus_tag="ACHLAM_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856307.1"
                     /translation="MRKRDDYYDGLIAGGTVAIAVATLLTLLITVFVNNKENSEFHEW
                     INSHCEKENVENTSFKCDDGDGNIYIVKKEA"
     gene            4077..4532
                     /locus_tag="ACHLAM_004919"
     CDS             4077..4532
                     /locus_tag="ACHLAM_004919"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO7856308.1"
                     /translation="MTTRKSGFNWYVLEFCSARTARVFSHLERLGVTFWCPLTCSHYR
                     RSDKNCFRRKEVPLFPGYVFIKIDFNKTHSTTVTSVPWVKSFVSFGGEPVPVCEEIIL
                     GLRRRETNYWGEYSPIRGIPHELAEILLENNPMKRSFLFFKYLSNTQNR"
     gene            4608..4787
                     /locus_tag="ACHLAM_004920"
     CDS             4608..4787
                     /locus_tag="ACHLAM_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856309.1"
                     /translation="MIYTKKQCVWLGFLLIGVAFLGFMADDFTDNIYTFIWIMDSFFG
                     VFMLFMGVFISDDES"
BASE COUNT         1401 a          952 c         1094 g         1380 t
ORIGIN      
        1 tttttttagg cggcaggtgt tacacggctg aaaaacttga aaaggattat ctggctgaag
       61 ttgctaatta cagtgacgac cgctgggaag caccgcaacg tgccgcacgt ctggcagctg
      121 ctgtaaagcg ttacaagaca tcagaaatgc tgcgttttat ttttgctacc attgcatacg
      181 atcccgatcc agatttaacc ccgctgaccg tgagacgttt gtgcaaggca ttgtttggta
      241 gaagcggaag ccagtggctg gtcgttgaag tttttggtga gaaggggcgt cagcaccgca
      301 gcgcggacag taacccggat atggtggaaa aaatggcagc cagatatcgt catgccgccg
      361 gtcagcactg gtccgcaacg ctggctgaaa ttgaacgggt aaagcgcatc taccaggcaa
      421 gaatcaaggc atcctgagac cggatgcctg gtatttgtct gggccattaa tgctaatgta
      481 tgcatacatt agcattaatg gccacatcct ggatgaggtt ctacgctgga actggagtct
      541 gaagtctaat ggaacgaaaa cgtacgttag tgaagtaact gtctgatata tcaaaacata
      601 atggagaccc tcaaacccca actacagccg aacgacaggc acaaaaaaac ccgactggta
      661 atcgggcttt tttgcatttt ccagtcttga gttggtggct ctgaccggag aaggttactc
      721 tacacctaac gtttacattt tattcactat ggtgctgtaa agcaactaaa tgtggttaag
      781 gaaccacatt tagttgtgta acgggtgttt tgtgtagcgt ataatcagtc agttagtcat
      841 gtagttggct tgcaatcctg ctcttcccgt atctgcgggt atgaacgatt ttaaagtcct
      901 ggctggataa acaggtggtg ctgtattcgg tgctctggca ggaggaggtc actctacacc
      961 taacaatgcg agagagccgt tgtgacaggc aatgtatgag taagcaacgg tgatttcctc
     1021 cagttttact ggagaaacgg atgattaatc tgttaatcat cgttctcagg gctgtggtcg
     1081 ccgttgcaaa cgcgctgatt gcaattctgg aactgattcg ccagttcatc gactgatgac
     1141 agcggaaacg taactaaggg caggaagtgc accgcttcct gccctttaac aaaggaggat
     1201 ttatggattt tatgagcatt ttagggcgct gtttacttct tcctcttccg gttgccatat
     1261 tcattgggtg cttactttct caggacgatg accgattttt tggcatgttt atgagtgtag
     1321 taagtgctgg gttgtcgtgg tatatcacta agccaaaacc acaaaaggct gaggtcgcgt
     1381 aagcggcctt ttatttcggt atttattagt tacaacattc acttttcttt tttaaagcaa
     1441 caccttagca tagccccacc catcataaat aatgtcagcc agtttattga gagcgccctg
     1501 ccctactcac tggagaattg ccgggattta cgttttattg cgctactgat tgtgccacgg
     1561 ctgcacccta gcaccttctg tacctgtgtc caggagctac cgctttctat caacctgata
     1621 attgcatcat gtctggcctg attaggttta cgctctttat atttgccgtc tttccttgct
     1681 ttctctatcc cctgtttctg gcgttcccgc cgttgttcat aatcccttct ggcaacagca
     1741 gccagcatat ccagcagcat atcgttaatg gctgaaaaca atcggttgtc aaactcactc
     1801 acactcaaaa agaaccagaa aacgtaccca taaccagaaa gtgacaatgt catacatgtg
     1861 ctttacagca taaagataat gtaaagataa atgcagcttt accacgtaac gcaaaaataa
     1921 agacgcaaat acctttacaa agtaaaggtc ttataaagac accattatct ttatttgtgc
     1981 tttactcggt aaagcttatg taatataaag acacggtaaa gacaaaacca tctttacaaa
     2041 aatcacacat tgtaaagaca atagagcctt tacacattct ttacatcata aagagaggaa
     2101 ttctgaaatg ggtaaaatct tgcttgtcgt atcagataag ggtggtgtag gcaaaagcac
     2161 ctacgtggct aatacaggct caatgctggt caacaaaggt aagtcggtaa tcatcctgaa
     2221 gacagataaa aaccacgatc tgttgagctg gaacgaaaag cggacagata acggcttact
     2281 cactatcccg gttcacgcag cttatgggaa cgtcagcaac gaaattaagc gccttagtaa
     2341 gctgtgtgaa gtgctcattg tcgattgccc cggccacgac agccaggagt tccgtagcgc
     2401 cctgacagta tcagatatcg tggtaacact agttaagccg tcctctgatt tcgaaagcga
     2461 aacactgacc agtgttacag agaaaatccg caccgctcag aaagctaacg cagccctgga
     2521 accgtgggtg ctgttcacaa gaatcaactc agctaaaccc cgccacagaa aagcggccat
     2581 tgatctggat aaattactgc gctcggacaa catctggatt caacctctga aaacccggat
     2641 atccgagctg gatgtgtatg aaagcgcctg taatgaaggg gcaggggtac atgatgttag
     2701 ccgggcatca agcctttcaa cagcgaaagc acagattgaa ctcgtggctc aggaaattgg
     2761 gattttgcag taatatcaat atgttaaatt attatcttta cattgtaaag ataatgtaaa
     2821 ggcggtatga tatttactat caatacatac cgtactaggg gaaatttcat ggctttaaaa
     2881 ctaaaccgtc ctaacatcga tgaatctcag caaccagcaa caaacgcaga aaccgcgcgt
     2941 tttatctccg gcgctaccag agcgcctgta gtgggtaaac caaagttgat gaatttcaga
     3001 ctgccagcgt actttgagga tatcctggaa gcggaggcgc agagaacagg ccagagcaag
     3061 acaaccgttc taaaggcagc gctggccgca ttcgataacc tggacgagaa cctgaaaaat
     3121 cattggctac ttgagtctgc aaagttaggt taacaagtaa ttcgcttatt taggtttaaa
     3181 aaatgagaaa gagagatgat tattatgacg ggttaattgc tggaggtact gtggcaattg
     3241 ctgttgcaac ccttctgaca ctgttaatta ctgtatttgt caacaataag gaaaattccg
     3301 agtttcatga atggataaac tcacactgtg aaaaagaaaa tgttgaaaac acttcattta
     3361 agtgtgatga cggggatggg aatatatata tcgtaaaaaa ggaagcgtaa ttaagggcgg
     3421 ggagggacca ttcctgttaa cgccctacag tcgtgacgaa tcagccagaa gtgctggata
     3481 tgtgtctgct gtttgccaga cgattttttc aagtaatacg cctgcaatcc cgattagcgc
     3541 aactacaacg cccgttctga taactgtttt cgctttcatt ttaacttaac tgatcaagac
     3601 gctgttaact ccatggtcgt acctgctgct ggggacagta catgtcagta cttaccaaac
     3661 aggcttcaaa aaagcaacag attcgctata tcggaagttt cgacggtttc ggctctcctc
     3721 cctgtatgac gactgcgtgt gcagtgctgg cttggttgtt caaaagttca acgaaaacag
     3781 tatgaaaaaa gaacatcaaa aaaaaatatt tatctggaaa ttggtgctaa cacgatctgt
     3841 tattttgttt ttttatcaat cggtagatct acgatctatt tgtgtggtta ttttcagtgt
     3901 aaaattctat agaaatggct taatatagat ctaagatcta catattcgca tatttacaga
     3961 taaaagcttt tgcgtaatat gttattacgc tattaaaagt tgttttgatt ctgcgcaaaa
     4021 aaacagagct acggatactt catgactgat cggggatccc cgaggcggag gtgtgcatga
     4081 cgactaggaa aagcggcttc aactggtatg ttctagagtt ttgttctgca cgtacagcac
     4141 gagtgttttc tcatctggaa aggttagggg taacattctg gtgtcccctg acttgttccc
     4201 attatcgtag atcagataaa aattgtttca gacggaaaga agtgccgctg tttcctggat
     4261 atgtgttcat aaagatcgat ttcaataaga cccatagcac aactgttacg tcagttcctt
     4321 gggtaaagtc atttgtgtca tttggtggtg agcctgttcc tgtttgcgag gaaatcattc
     4381 tgggattgag aagaagagag actaattatt ggggggaata ttctcctata cgcggaatac
     4441 ctcatgaatt ggctgaaatc ttgttagaaa ataatccgat gaaaagaagt tttttatttt
     4501 ttaaatattt aagcaatacg cagaataggt agttgaaaat cttggtttat tatcttttgt
     4561 gtattcgtgg agaactataa taaaataata aattaaaaga ggataaaatg atatatacaa
     4621 agaaacaatg tgtttggctt ggttttctat taattggtgt ggcgtttttg ggttttatgg
     4681 ctgatgattt tacagataat atctatacat ttatttggat tatggactct ttttttggtg
     4741 tctttatgtt atttatgggg gtgtttatat ctgatgatga gtcatgaatc aataaatcat
     4801 aggttatttg aaatgttatg ttttaat
//
LOCUS       DAZGAP010000279         4767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGAP010000279 DAZGAP010000000
VERSION     DAZGAP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.281"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..191
                     /locus_tag="ACHLAM_004921"
     CDS             <1..191
                     /locus_tag="ACHLAM_004921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856310.1"
                     /translation="CEAYHIEIMELLAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLG
                     TPMVIYGLVRPLTIETRD"
     gene            complement(410..1948)
                     /gene="aldB"
                     /locus_tag="ACHLAM_004922"
     CDS             complement(410..1948)
                     /gene="aldB"
                     /locus_tag="ACHLAM_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO7856311.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2056..3351)
                     /locus_tag="ACHLAM_004923"
     CDS             complement(2056..3351)
                     /locus_tag="ACHLAM_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO7856312.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(3481..4632)
                     /gene="yiaY"
                     /locus_tag="ACHLAM_004924"
     CDS             complement(3481..4632)
                     /gene="yiaY"
                     /locus_tag="ACHLAM_004924"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO7856313.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAEGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYIHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
BASE COUNT         1162 a         1258 c         1143 g         1204 t
ORIGIN      
        1 agtgcgaggc ttatcatatt gaaataatgg aactattggc caaactaatg aataactcca
       61 gtaaaacatc cacagtacag attaagcgta ttaaaccttc aattatctac cgtttattgc
      121 tgattggcct cggaacgcca atggtgattt atggcctggt tcgcccgctc accatcgaaa
      181 cgcgagatta aactcctggc aaaacacgcc ccagaaacgc acttctgttg acgtagtctg
      241 ggttattgcg cacgtaggtt taaaacgtaa attgtaacaa cgtgcgcttt gtttatgccg
      301 gatgcggcgt gtacgcctta tccggcctac ggtcaggtcc ccgtaggcat gataagacgc
      361 gtaagcgtcg catcaggcaa tgaataccca atgcgaccag cttcttatat cagaacagcc
      421 ccaacggttt atccgagtag ctcaccagca ggcacttggt ttgctggtaa tgctcaagca
      481 tcatcttgtg ggtttcgcgt ccgatgcccg actgtttgta gccaccaaac gccgcatgtg
      541 ccgggtaagc gtgataacaa ttggtccaca cgcgccctgc ctgtatgccg cgccccatct
      601 tataggcaag attaccgttg cggctccaga cgcccgcgcc caggccatat tgcgtatcgt
      661 tcgccagctc cagcgcttct tccatcgttt tgaaggtggt caccgccagc accgggccaa
      721 aaatctcctc ctggaacacg cgcatattgt tctgaccaaa caaaatcgtc ggttcgaggt
      781 agtagccgtc tttcagttca ccttccagca gcttgcgccg cccgccagtg agcacgtctg
      841 cgccctcttt tttaccgata tcaatgtagt tgaggatggt ttccagttgc ccgtgagaaa
      901 cctgcgcccc catttgcgtc acgctgtcga gcgggttacc gctgcgaatg ctttcgacac
      961 ggcggatggc gcgttccata aagcgttcgt agatagactc ctgcactaaa gcacggcttg
     1021 gacaggtgca aacttcgccc tggttaaagg caaacagtgc aaagccttcc agcgctttat
     1081 cgaaaaaggc atcttcttca tccattacgt cagcaaagaa gatatttggc gatttgccac
     1141 ccagctccag cgtcaccgga ataatgtttt gcgtggcgta ttgcataatt tgttggccca
     1201 cttccgttga gccggtaaac gccactttgg cgatgcgttt cgaggtcgcc agatattcgc
     1261 caatttcccc acctgcgccg ttgaccacgt tcaccacgcc cggcggcagt aaatcaccga
     1321 cgatttccat tagcagcagt acagaaagcg gggtaagacg tgcgggtttc agcaccacac
     1381 agttgcccgc cgccagcgcg ggagccattt tccagctcgc catcagcagc gggaagttcc
     1441 acggaataat ctgccccacc acgcctaacg gctcatggaa atgataggcc acggtttcgc
     1501 tatcaacttc actgatccca ccttcctgcg cccgaataca cgaggcaaaa tagcgaaaat
     1561 gatcaatcgc cagcggcaca tcagcagcac tggtttcgcg aatgggtttg ccgttatccc
     1621 aggtttcagc ggtcgctaac agttcaaggt tttgttccat tcgatcggca atcttaaaca
     1681 gaatcgccgc acgatcctgc accgaggtgt gcgcccattt atctttcact ttgtgcgcag
     1741 catccagcgc cagatcgatg tctcgtttgc ccgaagacgc cacttcgcac agcagctgcc
     1801 cggtcaccgg cgtcaggttc tggtaatact cgccatcggc aggcgctacc cattcgccac
     1861 cgataaagtt gtcgtagcgg gcttttaatt tgagggggaa accatactcg ccgggcttaa
     1921 tctgtgctga agggggatta ttggtcatga tcgtctcctt gcggtgtgag gtataacaag
     1981 ggtagacgtg actggcgaaa tcttcgccag tcggtaacag ctttacgacg gctatcacga
     2041 atttacgggc aaaagctaca gcgggtagtg tgcgacgtac acggcttgct ggttattaat
     2101 tcttttctct tgcgcgaatc cttctttaac aattgtccgt aaatcagctc gagccgtatt
     2161 atcagagacg cccaagctcg cgctaatttc agcaactgtg tactctttcc ccggattggc
     2221 gagcatcaca ttcaggagcg ttacctggcg cggattcagg cgtttaagca caccagagct
     2281 aaacagcaca ctatcgagtt ttgcgcttcg ttgtgtaatt tcatcgatat gctgctgcca
     2341 gttttgcaga gcacgtttaa tgacacccgc ctgatattca aggaaataag tcagatccat
     2401 accatctgat tcagtgtact ggtagctggc ggcatatttg accggtgcag catgaagcaa
     2461 acggctgatg gaaatatatt taaagacatc gtagcccgat ttcagcatat accagtaaaa
     2521 cagcgcccga ctggtgcgcc cgttgccatc gcgaaacgga tgttcatggg ccagcataaa
     2581 atgcagaatg caggcacgga ccaacggatg aatataccct tcatggctgt ttaaccactg
     2641 gcaaactttt tccagccgtt ccggtaacaa tgccgcagcg ggcggttgat gcactatatt
     2701 gccatcatag tcggcgatca caacatcatc tgtttcacga aattctcccg gacgatattt
     2761 cgcgtcattg attccaccca taccaatggc atgtaactgg cgaataagcg caggtgtcag
     2821 cggttctgcg agtaagtgcg gaatttccgc catcagccgg gcatttcccg caatcatatg
     2881 ctcgtcttca gtgcgtggcg accgccctgt ttccagcatt tttctcgcca ccagcgttgt
     2941 agtattcgca ccttccagtt gggaactggt gatcggctct tccaggcgta actgacttaa
     3001 ctcggcgccc gccccgcgta accgttcagt ctgatctttc agcgccagtg cagtggcatg
     3061 tttatcaaca agctcgcacg cttctgcaat caccggcgta atgttgaatc cggcctgttc
     3121 acccgcttca ttgcaataat taattcgttg gatcgccgcg ttgcgcgcca gacgggtaag
     3181 cgtccacgcc acatcgacgt tttctccttt tttgatgcgg tactgcagct catcaaaagg
     3241 cagataacgt cctttttcat cggtaacccg gttaaagcgc aggtgggcca ggagttcctc
     3301 acccgataag tgtgataaag gaataagtcc gggaggtgat ttgatagcca tgtgcttatc
     3361 tcaaaaaagc gcaatattga gatagtgtat agcaggaaag agaaactatc tcaaaaatcg
     3421 caaattttga gatagtttaa aaaatggctg acaattagac agccattgtg ggaatgatga
     3481 ttacatcgct gcgcgataaa tcgccataat ttcttcgtga gttgcctgaa tcgggttagt
     3541 aaacccacac gcatctttca gggcattggt tgccagcact ggaatatctt cttctttcac
     3601 gttcaggtca cgcaggcctg ccgggatatt cactttcttc gccagttcac ggatggcatt
     3661 gatgcacgct tctgcgcctt ccgcgtcatt tttgcctgtc acgttaacgc ccatggcagc
     3721 agcacagtca cgcagacgcg cagcagcgac tttgctgttg aacacctgaa cgtgtggcag
     3781 cagaacggca ttacatacac catgtggcag gttgtagaaa ccgcccagtt ggtgcgccat
     3841 cgcatgaata taaccaagag atgcgttgtt gaatgccatg ccagcgagga actgggcata
     3901 agccattgct tcacgcgctt tagcattgct gccatcttca acggctaacg gcaggttttc
     3961 ggcaatcatg gtcacggctt tcagtgcgca agcgtcagtg atcggcgtgg cggcaataga
     4021 aacatacgct tcgatagcgt gcgttaaggc atccataccc gttgcggcgg tcagtgactt
     4081 cggcataccg accatcagag atgagtcatt gacagaaagc aacggagtta catgtttatc
     4141 aacaatcgcc attttgatat gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc
     4201 agaggccgta cccgccgtgg tattgatggc gatcatcggc agctgcggtt ttgcagagcg
     4261 gtcaacgcct tcgtaatcgc gaatatcgcc gccattggct gccaccagcg caataccttt
     4321 tgcgcagtcg tgcggagaac caccgcctaa ggagatcacg ctatcgcaat tattttcttt
     4381 aagtaatttt aaaccttcgg cgacgttttc cgtggtgggg ttaggttggg tgccatcata
     4441 aataacgcta aaaatattgc gttcttccag tgctttttgc acatcacccg ccatacccaa
     4501 tttcgttaac atgttgtcgg tgacaattaa ggtacgggta aatccataat ctgccatcat
     4561 attcattgca tcattcaatg aatcagcacc aataacattc acggaaggaa taaaaaaggt
     4621 tgaagctgcc ataacacact cctgaaagtg taaaaaaggg cgggcagctt actgaggatt
     4681 ttcatttgaa aatatgatca acattaccgt ttattaaaat atttttaata aaaagcacgt
     4741 tacattcatc aaataacgtg ccttctt
//
LOCUS       DAZGAP010000280         4739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGAP010000280 DAZGAP010000000
VERSION     DAZGAP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.282"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..869
                     /gene="tcyJ"
                     /locus_tag="ACHLAM_004925"
     CDS             69..869
                     /gene="tcyJ"
                     /locus_tag="ACHLAM_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7856314.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            974..1960
                     /gene="dcyD"
                     /locus_tag="ACHLAM_004926"
     CDS             974..1960
                     /gene="dcyD"
                     /locus_tag="ACHLAM_004926"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO7856315.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKIGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            1975..2643
                     /gene="tcyL"
                     /locus_tag="ACHLAM_004927"
     CDS             1975..2643
                     /gene="tcyL"
                     /locus_tag="ACHLAM_004927"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO7856316.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            2640..3392
                     /gene="tcyN"
                     /locus_tag="ACHLAM_004928"
     CDS             2640..3392
                     /gene="tcyN"
                     /locus_tag="ACHLAM_004928"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO7856317.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            3622..4344
                     /gene="sdiA"
                     /locus_tag="ACHLAM_004929"
     CDS             3622..4344
                     /gene="sdiA"
                     /locus_tag="ACHLAM_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO7856318.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCTAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(4411..4635)
                     /gene="yecF"
                     /locus_tag="ACHLAM_004930"
     CDS             complement(4411..4635)
                     /gene="yecF"
                     /locus_tag="ACHLAM_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO7856319.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(4622..>4739)
                     /gene="yecU"
                     /locus_tag="ACHLAM_004931"
     CDS             complement(4622..>4739)
                     /gene="yecU"
                     /locus_tag="ACHLAM_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO7856320.1"
                     /translation="LKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT         1179 a         1101 c         1275 g         1184 t
ORIGIN      
        1 gagttgtttt tgtgtttaca aacaatggct ctacactgca aacagacata acaacattcg
       61 gggtaaatat gaaattagca catctgggac gtcaggcatt gatgggtgtg atggccgtgg
      121 cgctggttgc gggtatgagc gttaaaagtt ttgcagatga aggtctgctt aataaagtca
      181 aagagcgcgg cacgctgctg gtagggctgg aaggaactta tccgccgttc agttttcagg
      241 gggatgacgg caaattaacc ggttttgaag tggaatttgc ccaacagctg gcaaaacatc
      301 ttggcgttga ggcgtcacta aaaccgacta aatgggacgg tatgctggcg tcgctggact
      361 ctaaacgcat tgatgtggtg attaaccagg tcaccatttc tgacgagcgc aagaaaaaat
      421 acgatttctc taccccgtac accatttccg gtattcaggc gctggtgaaa aaaggtaacg
      481 aaggcaccat taaaacggct gatgatctga aaggcaaaaa agtgggtgtc ggtctgggca
      541 ccaactatga agagtggctg cgtcagaatg ttcagggcgt cgatgtgcgt acctatgatg
      601 atgacccgac caaatatcag gatctgcgcg taggccgtat cgatgcgatc ctcgttgacc
      661 gtctggcggc gctggatctg gtgaagaaaa ccaacgatac gctggcagta accggtgaag
      721 cattctcccg tcaggagtct ggtgtggcgc tgcgtaaagg aaatgaagac ctgctgaaag
      781 cggtgaatga tgcaattgcg gaaatgcaaa aagatggcac tctgcaagcc ctttccgaaa
      841 aatggtttgg tgctgatgtg accaaataat cagcataatg acaaaaaagg gcgctttcac
      901 tagcgccttt tttatttacg cgttttcagc gtgcataata agaaaatcac aacaacacac
      961 atcggaggct gttatgccac tgcataattt aacccgtttt ccacggctgg agtttatcgg
     1021 cgcgccaacg ccgctcgaat atctgccgcg cttttctgat tatcttggac gggaaatttt
     1081 catcaaacgg gatgacgtca ctcccatggc aatgggcggc aataaattac gtaagctgga
     1141 atttctcgcg gcagatgctc tgcgtgaagg tgccgatacg ctgattactg ccggggcgat
     1201 ccagtctaac catgtgcgcc agactgccgc agttgcggcg aaaatcggtc tgcactgcgt
     1261 ggcgctgctg gaaaatccta ttggcacaac cgcagaaaac tatttaacca acggcaatcg
     1321 tttgttgctg gatctgttca atacccagat tgaaatgtgc gacgcactga ccgatcccaa
     1381 tgcccaactg gaagagctgg cgacgcgagt cgaagcacaa ggctttcgcc cgtatgtcat
     1441 tccggttggc ggttctaatg ctttgggcgc gctgggttat gtggagagtg cgctggaaat
     1501 tgcgcaacag tgtgaagggg cagttaatat ttcgtcggtg gtggtcgcgt cgggcagtgc
     1561 cggaactcac gccggactgg ctgttgggct ggaacacctg atgcctgaaa gcgaactgat
     1621 tggcgtgacc gtgtcgcgtt ccgttgccga tcaattgccg aaagtggtta acctccaaca
     1681 ggcgattgcg aaagaactgg agctgaccgc atcagcggaa attttactct gggatgacta
     1741 ttttgcacct ggctacggcg tgccgaacga cgaaggcatg gaagcagtga aattgctggc
     1801 gcggctggaa ggcattctgc ttgatcctgt gtataccgga aaagcaatgg cggggctgat
     1861 tgacggtatc agtcagaaac gcttcaaaga tgaagggccg attctgttta ttcataccgg
     1921 cggcgcgcct gcgctgttcg cctatcatcc ccacgtttag ctccggaaac gctcatgcaa
     1981 gaaagtatac aactggttat tgattccttg ccgttcctgc tgaaaggggc tgggtatacg
     2041 ctgcaactca gtatcggcgg catgtttttt ggcttactgc tcgggtttat tctcgcgctg
     2101 atgcgccttt cgccaatctg gcccgtgcgc tggctggcgc gtttttatat ctctattttt
     2161 cgtggtacgc cactcatcgc acaactgttt atgatctatt acggcctgcc gcagtttggc
     2221 atcgaattag atcccattcc gtcagcaatg attggtctgt cgctaaacac tgccgcctat
     2281 gccgccgaaa cactgcgcgc cgccatttct tctattgata aaggtcagtg ggaagctgct
     2341 gccagtattg gtatgacgcc gtggcagacc atgcgtcgcg cgattttgcc acaggcggcg
     2401 cgtgtggcgt tgccgccgct gtcgaacagc tttatcagcc tggtaaaaga tacgtcgctg
     2461 gccgcgacaa tccaggtgcc ggagctgttc cgtcaggcgc agttgattac ctcgcgcacg
     2521 ctggaggtgt tcaccatgta cctggcggct tcgctcatct actggatcat ggcgacagta
     2581 ttatcgacgt tgcagaacca ttttgagaat caacttaatc gccaggagag agaaccaaaa
     2641 tgagtgccat tgaagttaag aacctggtga aaaaattcca cggtcagacg gtgctgcacg
     2701 gtatcgacct tgaggtgaag cctggcgaag tggtggcaat tatcggtcca agtggttccg
     2761 gcaaaaccac gttgctacgc agcataaatc tgctggaaca accggaagcg ggaaccatca
     2821 ccgttggcga tatcaccatt gatactgcac gttcattaag tcagcaaaaa tcgctgattc
     2881 gccagttgcg tcagcacgtc gggtttgtct tccagaactt taatttgttt ccgcatcgta
     2941 cggtgctgga aaacattatt gaagggccgg tgatcgtcaa aggcgaaccg aaagaagagg
     3001 ccacggcgcg cgctcgcgag ctgctggcga aagttgggct ggcaggtaaa gaaaccagct
     3061 atccacgtcg tttgtctggc ggtcaacagc agcgtgtcgc gattgcgcga gcgctggcta
     3121 tgcgtcctga agtgattttg tttgacgagc caacgtcggc gctggatcca gagctggtgg
     3181 gcgaagtcct gaacaccatc cgccagctgg cgcaggaaaa gcgcacgatg gtgattgtga
     3241 cgcacgaaat gagctttgcc cgggatgttg cggaccgggc gatctttatg gaccaggggc
     3301 ggatagtcga gcagggggcc gcaaaagcgt tatttgccga tcccgagcag cctcgcaccc
     3361 gccagttcct cgagaagttt ctgctgcaat aataagaaaa aatcagcccc gacgattcac
     3421 ctgtcggggc ggtgcgccac ttcaaacgtg ataaaactgc ttaacaaatc agcataactc
     3481 attaataaca taagagaatg cgatggcttg caaaagtaat tcattgcctg aataatataa
     3541 attatatata aatcttattt atgtgatagt ttgaattatc attataaatg atactcactc
     3601 tcaggggcgt tgcggtttac tatgcaggat acggattttt tcagctggcg tcgcacgatg
     3661 ctgttgcgtt ttcagaggat ggaggccgca gaagaggtct accatgaaat agagcttcag
     3721 gctcagcagc tggagtacga ttactattcg ttatgtgttc gccacccggt accattcacg
     3781 cgacctaaag tggctttcta caccaattac cctgaggcgt gggttagtta ttatcaggca
     3841 aaaaactttc tcgcaattga tcctgtactg aaccctgaaa actttagtca gggccattta
     3901 atgtggaatg atgacttatt cagcgaagca cagccgttat gggaagctgc gcgcgcgcat
     3961 ggtttacgcc gcggtgtcac tcagtattta atgctgccaa accgggcgct gggctttttg
     4021 tccttttccc gttgcactgc gcgcgaaata cccattctta gtgatgaact gcaattaaaa
     4081 atgcagttac tggtgcgcga aagtctgatg gctctgatgc gtttaaatga tgaaatagtg
     4141 atgacgccag agatgaattt cagcaagcgc gaaaaagaaa ttctgaaatg gacggcggaa
     4201 gggaagacat cagcagagat agcgatgatt ttgtcaatct ctgagaatac ggtcaatttc
     4261 catcagaaaa acatgcagaa aaaaatcaat gcaccaaata agacccaggt ggcctgttac
     4321 gcggccgcta ctggcttaat ttgatcgctt ttctttctgc gtgccagatg caaaaaccgg
     4381 ctgaaaggca cgctctcagc cggttttata ttactgacgg taggcttgtt taatttgctt
     4441 aaccgttttg gaaaatactg cagcctgggt ttcgtcttcg atcagcgcaa gctgtttttc
     4501 catctttaac atcacgcggc ccgcgtcagc ttgtcccatc gcctgcagca tcagcgtcag
     4561 catcgctttc aggcaggaga cttcatttgc cagttcttga ttattctcgg cagtagaaaa
     4621 atcaggcgta ctcatttatt ttcctcgtca tgttgcaatg aaaatttgcg gtgaaaaatg
     4681 ttaaggcggc ggagtatacc ataagctttg ctaaaaatag cagtggttgt tttttgagc
//
LOCUS       DAZGAP010000281         4714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGAP010000281 DAZGAP010000000
VERSION     DAZGAP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.283"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..752
                     /locus_tag="ACHLAM_004932"
     CDS             3..752
                     /locus_tag="ACHLAM_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7856321.1"
                     /translation="MKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSEY
                     YLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPAQ
                     NILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEITQ
                     EDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTLS
                     VERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            766..1842
                     /locus_tag="ACHLAM_004933"
     CDS             766..1842
                     /locus_tag="ACHLAM_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO7856322.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWSGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            1842..2840
                     /locus_tag="ACHLAM_004934"
     CDS             1842..2840
                     /locus_tag="ACHLAM_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO7856323.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            2845..3759
                     /locus_tag="ACHLAM_004935"
     CDS             2845..3759
                     /locus_tag="ACHLAM_004935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO7856324.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
     gene            3787..>4714
                     /locus_tag="ACHLAM_004936"
     CDS             3787..>4714
                     /locus_tag="ACHLAM_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO7856325.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKST"
BASE COUNT         1374 a         1007 c         1082 g         1251 t
ORIGIN      
        1 tgataaaaat ttataaagcc agtaaaggtc agctaaagca tcgtgaaatc tttgatgaaa
       61 taaagaaaaa agtagataac ggtgaatgga aagaaggcaa agccataccg acagaaaggg
      121 aattgtcaga atattattta agtagcagaa caactattcg aaaggcaata gaaagtctta
      181 aacaacgcgg ctacctgcat agtgtccacg ggcaaggaac atttgtattg ccttctcgta
      241 gtagagaaaa tcatttattg catagtttta ccgacgatat taaagctcga ggtggtatac
      301 cagcacagaa tattcttgag ataggctatg ttcctgtcag tgatgttatc agaaaaaatc
      361 tcgacctgga tatccatgtc caaaccgtat tctgtataaa acgcgttcgc tatatgggaa
      421 gtacccccct gggtattcag acatcatggc ttgcattaaa tgacaggcaa gaaataaccc
      481 aagaagatct gctggcgacg ggttcgctct atattttact ggaagaaaaa ctcgctatta
      541 aacctttaga agcaacagaa attatctctg cccggcttcc ctcgcccctt gagcgtcaat
      601 tactggaact gactgccgat gatgtcgtat taagttgtac ccgcgtcacg ttatccgtgg
      661 aacgcaaacc aatggagtat gtagagatgg tgtatcccgc aagccggtac tcctacaaag
      721 cacgaattac aaaagacagt tttagcgtgt aacagagaga atattgtgaa caaaatagaa
      781 caagctgtaa tttataacaa aatattaggt tctttagcct gcgcaggtat gggcgatgca
      841 ttaggcgcag cgactgaact ctacagcatt gatgaaataa aagctcaatg gggaggtttt
      901 ttaaatgcct ttgtttctcc tcctgccgat acattcgctg gctcattaaa tggtattgcg
      961 ggacttatca ccgacgattc cagccaaatg tatgtgtttt cagaagggtt aattgaggct
     1021 ggatttgata acttcaccaa taatgactgg ttagcttgtc ttctgcgttg ggccgatatg
     1081 cagccctacg caaattataa aggccccaca acagaacaaa tcgtcaaggc actaaaagaa
     1141 ggtagaccaa caaacaccat tgggcgtatt ggaacatctt ctcgtcaggc acccaatgtc
     1201 ggcaccacga atggagcagg aatgcgtgta gcccctgcag gattgatctg gtcaggcaag
     1261 aaagaaaaag cgtgtcacct ggcgctaata acttgcctac cttcgcatga caccaatatt
     1321 gcgattgcca gcgcttgcgc catcgctgcg gcgacatcac aagctatgtt accagaagct
     1381 tcgctcacat cattactgga cgccgcaatc tggggagcga attacggcga acgcctggcc
     1441 aaacaatatg cacgatgtgt cgcagggcca tctatcgcca tgcgcatcca acttgcagcg
     1501 gacatcgcca gacgagccaa tgacctggag tcttgtctgc gagaaatgga aggtttggtt
     1561 ggtaactctg ttgccgccca tgaatccatt cctgcggcaa tcggtctatt gctgtactgc
     1621 aaaggagaac cctgggaaac cattcatgcc tgcgccaata ttggcaatga caccgatagc
     1681 atcgctacga tggcaggcgc aattgcaggg gcatggcgcg gttttgatgc gctccctgaa
     1741 gataaatatg cctttttccg cgccgtaaat aataaagatt tcgatattga agcgatcgca
     1801 tcgggtctga cattgcttgc attagaggca caggagaaat aatgaacatg ccagcccgta
     1861 ctcctgttat gccattagcg caaactcgtg aggagataaa tgctacggcg gcatcgttac
     1921 gccatttggc agctatctgg ggtgaatgtt tttctacttt tcagctcgat aaacgtgctg
     1981 ttgaacgcgt ctgtatagtg ggatcgggcg actcctggac ggtggcacta tgtgccgccg
     2041 cctggctggg gaaatacaca catctgtttt gttacgcatt gcaaacgtgg gattttcttc
     2101 agactgattt aacccgttat cagaaagaaa cgttaatcat tattctcagt gcaagtggca
     2161 gaccatcaat gaccgttgac gccctctgcc acgcagtatg ctcaaacgcg caagtgctgg
     2221 gtgtgacgaa ttgccctgga acaccatttt gcgcaataac agaaaacatg ctctatacat
     2281 gggcgaataa gcagggtatt ccaacgcaaa gtagtagcgt cacactttat gccctcctgc
     2341 gcttagcgca aaaattatgt ccaggcttaa caccactgca gattgaagat gatttggaag
     2401 ggaaatttag ccagattaat cagcactggc agcaaaagaa gcgccatttt taccaacaaa
     2461 aagagataac gtttcttggg agtggcttaa gttggggact ggcaatatcg gggagcaatt
     2521 tactttcttg cggcccacag attcgtgcga cagcccttcc actggaagaa ttttatcatt
     2581 cattacgctt acatcaggct ggtgtcggtc agcattatat tttactacca gccacatcct
     2641 gcgagtcccc attttacctt gcaacccaaa aagcaattgt tgaacaggga gcgacagcag
     2701 aattaatcag ttttatacct gacgccagtg agtccaacaa tttatttctg gtcatgcagt
     2761 ggttatatga aatgtgctgg catttaagtt gtgattatgt cgacgcaggc gggcaacgcg
     2821 ttagccatag ggagaaataa gactatgaag aaatttgatg tcgccgcttt aggcagtgga
     2881 aatatagata tttttttatc tatcccgtca ctccccactc gtggcggtaa agttgtcggg
     2941 acacgtctgg gtgaacaagc tggcggcaca gtcgccaaca gtgcctgtgc gatgggacaa
     3001 ctgggtctca atgtggtatc tgtttcatgt gtgggcaatg atcattcagc ctcgatcatt
     3061 cttgatggat ttaaaaaata tcatgtaaat tgtgatttcg ttcaggtaat tccggaattg
     3121 attgccaata ccgcgattat ctttattgat gaattaggtg aaaaaacgtt ggtttactcg
     3181 cctggtagtg atcatgagtg ggataaagaa aaagccctgc aggcaattgc gcaaagccgt
     3241 tatttttata cgatgccagc agatatcgag aaatttcgta tgctggccga atattcccgt
     3301 agccagatga caaaagttgt cgttgatatt gagcctcata tcattgcaac acctgaacac
     3361 cttgccagaa ttttacacct tgctgatatt gcgatattta attatgacgg ttttattcgt
     3421 ggctatgcaa cagagccaga ttttacactt ctgcatgata tacaagacga gtatcaactt
     3481 gacgctgtcg tggttacgct ggatgttagg ggagtaattg cagtcaaagg caatgagcaa
     3541 gtaaaaatag gtagttatac aattcccgtt attgatacca caggcgctgg agatactttt
     3601 aatggtgcat ttgtttatag cttaataaaa aatatgccct taattgaagc actaaaattt
     3661 gcttctgcca cagcagcaat aaatattact gcattaggtg ccagaggtca tctgccgaca
     3721 ccatcggagg ttaatctttt tctttcacaa cacgactaaa atcatatata tacaaaggta
     3781 aatatcatga aaataagata tgccgctatt tttctggcat taccgctgtt atgcacaaca
     3841 gcgcaggcaa acagttttct tcattatgct ggggagttta acttccggac gggagaaaag
     3901 cagaacaatg tcacaatcgc ggggctatca gccatcacat ttgatgcaaa aaaaaatatt
     3961 ttctatgcta ttaacgatag cagaaacaat aataaagaag gagatgccag tctttataca
     4021 ctaaaaatac aggtatcatc caggggcatt gagcaagtga attttctcaa ccagcgtcca
     4081 ctgcttgatg caaaacagca acctttcgtg accaatacgg tggatgccga aggtctggcc
     4141 ctgacacaca atggtaaatc attactctgg agttcggagt taggtgctcc actgcgtctg
     4201 agcactctcg atggcgtaat ggagaaagat tttacgtctt tatttcctgc tcgctttaat
     4261 attagttctg acaaagagag cagcaatggt attcgcagcg gcaacgcctg ggagggcctg
     4321 acagttacac cagatggaaa atcacttttt atcgccgttg aaagcagcct taaacaggat
     4381 gggcctattg caagtccaat aaattcaggc acatcacgct tactgcaatt ttctatagat
     4441 gcagacggtc gaccttcaaa gcaattgcat gaatatctct atattactga tcctgttccg
     4501 caagtgagta agttcggtat taatgataat ggcgtatccg aggtcatggc gctgaatgat
     4561 caccaattgt tggtcatcga acgttccggc agaaacgtca gtgctgggtt caatgactgg
     4621 gattactctg tcagagttta tatggtcgat ttaactgctg ccagcgatat caagaatatt
     4681 gacagtctgc aagattggtc caataaatcc acgt
//
LOCUS       DAZGAP010000282         4697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGAP010000282 DAZGAP010000000
VERSION     DAZGAP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.284"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..1114
                     /gene="pstS"
                     /locus_tag="ACHLAM_004937"
     CDS             74..1114
                     /gene="pstS"
                     /locus_tag="ACHLAM_004937"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO7856326.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1201..2160
                     /gene="pstC"
                     /locus_tag="ACHLAM_004938"
     CDS             1201..2160
                     /gene="pstC"
                     /locus_tag="ACHLAM_004938"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO7856327.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALTVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2160..3050
                     /gene="pstA"
                     /locus_tag="ACHLAM_004939"
     CDS             2160..3050
                     /gene="pstA"
                     /locus_tag="ACHLAM_004939"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO7856328.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3141..3914
                     /gene="pstB"
                     /locus_tag="ACHLAM_004940"
     CDS             3141..3914
                     /gene="pstB"
                     /locus_tag="ACHLAM_004940"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO7856329.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            3929..4654
                     /gene="phoU"
                     /locus_tag="ACHLAM_004941"
     CDS             3929..4654
                     /gene="phoU"
                     /locus_tag="ACHLAM_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO7856330.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
BASE COUNT         1080 a         1193 c         1303 g         1121 t
ORIGIN      
        1 ctgtcacctg tttgtcctat tttgcttctc gtagccaaca aacaatgctt tatgaatcct
       61 cccaggagac attatgaaag ttatgcgtac caccgtcgca actgttgtcg ccgcgacctt
      121 atcgatgagt gctttctctg tgtttgcaga agcaagcctg acaggtgcag gtgcaacctt
      181 ccctgcgccg gtgtatgcca aatgggctga cacttaccag aaagaaaccg gtaataaagt
      241 taactaccag ggtatcggtt cttccggtgg cgtaaaacag attatcgcta ataccgttga
      301 tttcggtgcc tctgacgcgc cgctgtctga cgaaaaactg gctcaggaag gcctgttcca
      361 gttcccgacc gtgattggcg gcgtggtgct ggcggttaac attccagggc tgaagtctgg
      421 cgaactggtg ctggatggta aaaccctcgg cgacatctac ctgggcaaaa tcaagaagtg
      481 ggatgatgaa gccatcgcca aactgaatcc gggtctgaaa ctgccttcac agaacattgc
      541 tgtagtacgc cgcgcagatg gctccgggac ttccttcgtc ttcaccagct acctggcgaa
      601 agtgaacgaa gagtggaaaa acaacgttgg tactggctct accgtaaaat ggccgatcgg
      661 tctgggcggt aaaggtaacg acggtatcgc cgcgttcgtt cagcgtctgc cgggtgcaat
      721 tggttacgtt gaatatgctt acgcgaagca gaacaacctg gcgtacacca aactgatctc
      781 cgctgatggc aaaccggtga gtccgaccga agaaaacttc gctaatgcag caaaaggcgc
      841 agactggagc aaaacattcg ctcaggatct gaccaaccag aaaggcgaag atgcatggcc
      901 tattacctct accacgttca ttctgatcca taaagatcag aagaaaccag aacaaggcac
      961 cgaagtgctg aagttcttcg actgggcata caaaaccggg gctaaacagg cgaacgacct
     1021 ggattatgcc agcctgccgg atagcgtagt tgaacaggtt cgcgctgcgt ggaagaccaa
     1081 tattaaagac agtagcggca agccgctgta ctaataaaac tccaggccgg gtacggtgtt
     1141 ttacgccgca tccggcatta caaaatgact ttgtaaacgc gtttaactga agagtaactt
     1201 atggctgcaa ccaagcctgc ttttaaccca ccgggtaaaa agggcgacat aattttcagc
     1261 gtgctggtaa aactggcggc gctgactgtg ctattgatgt tgggtggcat tattgtctct
     1321 ctgatcatct catcctggcc gagcattcag aaatttggtc tggctttcct gtggaccaaa
     1381 gagtgggatg cgccgaacga tatctacggg gcgctggtgc cgatctacgg tacgctggtg
     1441 acctcgttta tcgcgctgct gatcgccgtc ccggtgagtt ttggtatcgc cctgttcctg
     1501 accgagcttg cgcctggctg gctgaaacgc ccgctgggta tcgccattga gctgctggct
     1561 gccattccaa gtatcgttta cggcatgtgg ggcttgttta tcttcgcccc gctgttcgcc
     1621 gtttacttcc aggagccggt cggcaatatc atgtcgaata tcccgattgt cggcgcgctg
     1681 ttctccggcc cggcctttgg tatcggtatc ctcgcggcag gcgtgatcct cgccatcatg
     1741 attattccgt acattgcggc ggtaatgcgc gatgtattcg aacaaacccc ggtgatgatg
     1801 aaagagtcgg cctacggtat tggctgcacc acctgggaag ttatctggcg tatcgttctt
     1861 ccgttcacca aaaatggtgt tatcggcggc atcatgttgg ggctggggcg cgcgctcggt
     1921 gaaaccatgg cggtgacctt tatcatcggt aacacctacc aactcgacag cgcctcgctg
     1981 tatatgccgg gcaacagtat cacctctgcg ctggccaacg aatttgcgga agcagagtcg
     2041 ggtctgcatg ttgctgcact gatggaactg ggcctgatcc tgtttgtgat taccttcatc
     2101 gtcctcgccg catcgaagtt tatgattatg cgcctggcta agaatgaggg ggcacgctaa
     2161 tggctatggt tgaaatgcaa accactgcgg cgctggctga atcccgccgt aaaatgcagg
     2221 cgcgtcgccg ccttaagaac cgtattgcgc tgacgctctc gatggcgacg atggccttcg
     2281 gtctgttctg gctgatctgg attttaatgt ccaccatcac tcgcggtatc gatggtatgt
     2341 cgctggcgct gttcaccgaa atgacgccgc cgccaaatac ggaaggcggt ggtctggcga
     2401 acgccctggc gggtagcggc ctgttaattt tgtgggccac ggtattcggt acgccgctgg
     2461 gcattatggc ggggatttat ctggctgaat atggtcgtaa atcctggctg gcagaagtga
     2521 ttcgcttcat taacgacatt ttgctctccg caccgtcgat tgtggttggt ctttttgttt
     2581 acaccatcgt ggtggcgcag atggagcact tctccggctg ggcgggcgtg attgcgctgg
     2641 cgctgttgca ggtgccgatt gttatccgca ccaccgagaa catgctgaaa ctggtgccgg
     2701 acagcctgcg tgaagcggct tatgcgctgg gtacgccgaa gtggaagatg attgccgcga
     2761 tcacgctgaa agcgtcggtg tccgggatca tgaccggtat cctgctggcg attgcccgta
     2821 ttgctggtga aaccgcgccg ctgctgttta ctgcgctctc caaccagttc tggagcacgg
     2881 acatgatgca gccgatcgcc aacctgccgg tgacgatctt taagtttgcg atgagcccgt
     2941 ttgcggaatg gcagcaactg gcctgggccg gggtgttgat cattaccctg tgcgtactgc
     3001 tgctgaacat tctggcgcgc gttgtttttg cgaagaataa acacggttaa cactttgctg
     3061 gatgcggcgt aaacgcctta tccggcctac ggtaagcctg attcgcgaag tgcatcaggc
     3121 acgatgagga aaagattgca atgagtatgg ttgaaactgc cccgagtaaa attcaggttc
     3181 gtaatttgaa cttctactac ggcaaattcc atgccctgaa aaacatcaac ctggatatcg
     3241 ccaaaaacca ggttacggcg tttatcgggc cgtccggctg cggtaaatca acgctgctgc
     3301 gtaccttcaa caaaatgttt gaactgtacc cggaacagcg tgcggaaggt gaaattctgc
     3361 tcgatggcga caacatcctg accaactctc aggatatcgc cctgctgcgt gcgaaagtgg
     3421 gcatggtgtt ccagaaaccg acgccgttcc cgatgtccat ctacgacaac atcgcttttg
     3481 gcgtgcgtct gtttgagaag ctctcccgtg ctgacatgga cgagcgcgtg cagtgggcat
     3541 tgaccaaagc cgcattgtgg aacgaaacca aagataaatt gcaccagagc ggttactctc
     3601 tctctggtgg tcagcaacag cgtttgtgta ttgcgcgtgg tatcgccatt cgcccggaag
     3661 tgctgctgct cgacgaacca tgttcggcgc tcgaccccat ctccaccggg cgtattgaag
     3721 agctgattac cgaactgaag caggattaca ccgtggtgat cgtcacccac aacatgcagc
     3781 aggctgcgcg ttgttccgac cacacggcgt ttatgtacct gggcgaattg attgagttca
     3841 gcaacacgga cgatctgttc accaagccag cgaagaaaca aacagaagac tacatcaccg
     3901 gtcgttacgg ttgattcagg agtgcgttat ggacagtctc aatcttaata aacatatttc
     3961 cggccagttc aacgccgaac tggaaagcat ccgcacgcag gtgatgacca tgggcggcat
     4021 ggtggagcag cagctttctg atgcgatcac tgcaatgcac aaccaggaca gcgatctggc
     4081 gaagcgcgtc atcgaaggcg acaagaacgt caacatgatg gaagtggcga tcgatgaagc
     4141 ctgcgtgcgc attattgcca aacgccagcc gacggcgagc gacctgcgtc tggttatggt
     4201 gatcagtaaa accattgccg agctggagcg tattggcgac gtggcggaca aaatctgccg
     4261 tactgcactg gagaaattct cccagcagca tcagccattg ctggtaagtc tggagtcgct
     4321 gggccgtcat accatccaga tgctgcacga cgtgctggac gcgttcgcgc ggatggacat
     4381 tgatgaagcg gtacgtattt atcgtgaaga taaaaaagtc gatcaggagt acgaaggtat
     4441 tgttcgtcag ctgatgacct acatgatgga agattcgcgc accattccaa gcgtacttac
     4501 cgcgctgttt tgtgcgcgtt caatcgaacg tatcggcgac cgctgccaga atatttgtga
     4561 gtttatcttc tactacgtga aggggcagga tttccgtcac gtcggtggtg acgagctcga
     4621 taaactgctg gcagaaaaag atagcgataa ataattcacc aagtaaatcc caataattta
     4681 attattggga tttgtta
//
LOCUS       DAZGAP010000283         4659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGAP010000283 DAZGAP010000000
VERSION     DAZGAP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.285"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            443..1009
                     /locus_tag="ACHLAM_004942"
     CDS             443..1009
                     /locus_tag="ACHLAM_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7856331.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERIAECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRNLQK"
     gene            1267..1842
                     /locus_tag="ACHLAM_004943"
     CDS             1267..1842
                     /locus_tag="ACHLAM_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856332.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSCSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            1911..2147
                     /locus_tag="ACHLAM_004944"
     CDS             1911..2147
                     /locus_tag="ACHLAM_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7856333.1"
                     /translation="MLTSITGHDSILLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2429..2941
                     /locus_tag="ACHLAM_004945"
     CDS             2429..2941
                     /locus_tag="ACHLAM_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856334.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHRGATIRCLLLLSQTSICH
                     PRASVTVDEECSQLVDLLQQTWKVISAGGQCRVEKRFQVVRGDTSGQYVALKTVVQSF
                     VLPVMTTINW"
     gene            3030..3653
                     /locus_tag="ACHLAM_004946"
     CDS             3030..3653
                     /locus_tag="ACHLAM_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7856335.1"
                     /translation="MRTRIDYLANKYSFTELNESPRLRWQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRQSGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            3749..3982
                     /locus_tag="ACHLAM_004947"
     CDS             3749..3982
                     /locus_tag="ACHLAM_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856336.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(4002..4214)
                     /locus_tag="ACHLAM_004948"
     CDS             complement(4002..4214)
                     /locus_tag="ACHLAM_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7856337.1"
                     /translation="MSEDVPLPKANQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPREEKIPAGNHTA"
BASE COUNT         1125 a         1085 c         1133 g         1316 t
ORIGIN      
        1 tcttgaacat gagttgtttt taaccgatta aatgagtgcg tgtaagttta ttgggttaca
       61 ggtatatgtt ggccttgtac acgttttttc agactcactg agagcaagtt tgtagtttat
      121 gatgttattg gtcgtaatat gttttttgaa ctattggtcg tgggttttta tcgcgtgttt
      181 tgcagtattt cgtgatgtat gtttattgat tctgtatttt attcgctcct cgtttatctc
      241 atctctttag ctaataccat cagataatcc atttctttct gcataatgct gcgtatcgtt
      301 aataacccgt cgtatccatt ctgctacagc atgcctgata aataccatct gtaagttatt
      361 accgttttag atctgattat gagcgaaagc attaattcgt tcacagagct taaaacatca
      421 ttaactttca ggagtcatca acatgcctaa atcttacaca ccaaactggt tttttaccgc
      481 tttacttgac aatcacatca atcaaatgct ggcacgttat tcctgcctgc gagctttacg
      541 gatggatttc ttctacagga aagatacgcc tgatttctta caacctgatc atcgctggct
      601 tgaattgcag ttgcgtatgc tgctggagca ggtggaacaa tttgaaaata tcgttggctt
      661 cttctgggtg attgaatgga cggctgatca tggttttcat gcacatgcgg ttttctggat
      721 cgatcgtcag agggttaaaa aaatatatcc ctttgcggag cggatagctg aatgctggcg
      781 gtctattacg cataacagcg gttcggcaca ccgctgtaca tatcagccgc attatacata
      841 caacatcaac attcctgtgc gccacaacga tcctgaaagc atcgataata ttcgcggtgc
      901 cctgcattat ctggcgaaag aagaacaaaa agacgggctg tgtgcctacg gctgcagtga
      961 agttcctgag cgtcctgccg cagggcgtcc acgtaatctt cagaagtgaa gcttaaacct
     1021 tgagtttcct ggcccggaaa cactccgtta gtaaaaactt accgccctgc ttaatgatgt
     1081 gaaccgaagt caatggagat cattcaccct gaaccacatc cggtgttttg ttccttgtca
     1141 gttacctgat cttcccgttt tgccccggtt tttcacttat tccatcaacc ccatccgcaa
     1201 accataacac gtcagcttat tcacgggcag gacgcattgt gggctgcgca taatggagca
     1261 tatcttatga atgctatccc ttatttcgac tatagcctgg cacccttctg gccatcttat
     1321 cagagcaaag tcatcggcgt tcttgagcgt gcgctgcgtg agcagtccgg ctcacggata
     1381 cggcggatcc tgcttcgtct gccgtgtgaa tatgacaata ccttcaacag cagaacgacc
     1441 tggtttggta tggattttat cgaaaccgtc agtgcgctga tgaatgcgaa acccggacgc
     1501 gacctttgct ggctcctgac ccgtcatcct gaaaagccgg aataccacgt ggtgctgtgt
     1561 gtcagacagg agtatttcga cggccctgaa ctggatcggc taattctgga tgcctggagc
     1621 aacgtactgg gcttcgcgtc accgggtgaa gcagcaccgt accagaagca gatcaccagg
     1681 gatgtactgc tggacagttg ttcaccggac tgcgaagaca tcctcaaaga cttgatctgg
     1741 gcgttcagtg atttcgcccg cgatcgtcgt ggtgtgtgcg atccggaagc ccgttgcctc
     1801 gccggtaatc ccggctatcc cggatctgca ggactgttct gaaagctcag ttcagcagat
     1861 tcaccagcat ttgcccaacc tccataaatc tcacagagga catcctgact atgttgacct
     1921 caataacagg ccacgacagt atattgctgc gtgccgacga tcccctgatc gacatgaact
     1981 acatcaccag tttcaccggt atgaccgata aatggtttta caagctgatc agtgaaggtc
     2041 attttcctaa acccataaaa ctgggtcgca gcagccgttg gtacaaaagt gaagtggagc
     2101 agtggatgca gcagcgaatt gaggaatcac gaggagcagc tgcatgaaac gtgttgtgat
     2161 gccggtacgc tggcaatgtg caaaatgtcg gcgctggtat tgtggagatc agccctgctc
     2221 ctggtgctgg cgctgacaac tcccgctcat ctttctgctg actcctctgg tcagccaact
     2281 gtttgtcatc atttcctgac tgattcgtca ttcccttcgt cccgccagtc actggtgttt
     2341 gtatcgtatc gtgccggtgt tgatgtgcgc tttccgtgtg tctgcaccgg cttaacaaaa
     2401 ttcaacaggg tttgaaaagg aacatttcgt gcaaataacc aaagccttaa tttcagagcc
     2461 gggagacatc cggcgttttg ttcagcaggc ggtggaccac tggccaaatc tgttggcatt
     2521 tcacttcacg ctctattcgg cagaaggaaa catcaacggg caacagattc atgcattctg
     2581 tacttccttt taccgacagg tgcacgaacg tattactgag cgtaatcaca cggccagtcc
     2641 atcctcgccg gtggtattac gctggttgcg ggaacaacat agaggagcaa caattcgatg
     2701 cctgttgctg ctcagccaga cgagtatttg tcacccgcga gccagtgtca cagttgatga
     2761 agaatgttca caactggtgg atttactaca acagacctgg aaggtgataa gcgctggcgg
     2821 acaatgccgg gtggaaaagc gttttcaggt cgtccggggt gatacatctg gccagtatgt
     2881 tgcgttaaaa acagtcgttc aatcctttgt gttaccggtt atgacaacaa tcaactggtg
     2941 atggtcgtgt gaaccagctg cgcttccgtt gattcttatg gactgataaa gttttgtcag
     3001 atgaattttt atacagaatt cttttcagta tgcgtacacg aattgattat ctggcgaata
     3061 aatacagttt tacagagctg aatgaatccc cccgtcttcg ctggcagtgg caggatgttc
     3121 tggaggagtg ccggcagaca gaggccgggc cagaagaacg gctgcgtatt gctctgctga
     3181 atgtggatta cgtcaccagt tttgaactgc cttttcgctt gttgcttatc cgtacaccac
     3241 aactgattgc cgcgctgcgt gaagaatggg acatcagcca gaaaaatgtg atgttcaacg
     3301 ataaacgctt tggctgcgtg tacagcctga aggccagcct ttccggtgtg ccggatacat
     3361 accggtatca tctgtctcac cgtattcgtc gcgtggttgg aaatgaaagc acatcatcgc
     3421 catatcagca ggttgccagg gaagtgaaag cgccccgtga acggctgaag tatgcgctgg
     3481 aggcgggctt actggtgacc gcactggacg gccttttctg gtctggcagt cagcgtattg
     3541 cggctgatgt cctgagacta agacagagcg gaatgccggt ggtgacaacg tccgtggaag
     3601 tgagcgataa cctgacggga acaacccgca aaataccggc ataccatctc tgatacggcc
     3661 atcaggggga atattgctct ggcagcagca gtacgacatt ctttatactt tatccccgtg
     3721 cctgaaaaca tgtgctaagg acacgggaat agcaaggtcc gattacgata ttatcaatct
     3781 gtctccggaa catgaactga atgagtggct ggcagagaga ggttatgccg gactggcgga
     3841 taaccggaac cgcctggcag aggtggttgc ctgcaaattg caggacagct tctgtatgaa
     3901 cgtttcctgg gatgctctga acgcagcata cagtgaacat cccgagtggt tttcagggct
     3961 tgtctccggg gatgagaatt aaaaagagga ttatgctgcc atcaggcagt gtgattgccg
     4021 gcagggattt tttcttcccg gggttcgggt ttgaattttc ccaggaagtt gtacatcggc
     4081 cgcatgcagg gatgcggata gccgtcccgc ataaatacca cccgggtttc ctcaacactg
     4141 gtcacggtga caagcgcacc atgatcgtct ctgtatcgct gattggcttt cggtaatgga
     4201 acatcttcag acatagattt cagaacggta gcagttaact aaaatttcgt gtgatattgc
     4261 tgaatcagga gtctctgcct cctgatgtgt tttgttaatc ttctgttttt aagaaatatt
     4321 ttctctctct cttctttatt ttccgtgtct tttattgctc tcctctttca tgtccttctc
     4381 tttttctgat acgcccttaa atgctgccga atcatcactc ctcgtgaatg tcagtgctgg
     4441 taatcattcg gtataaccat ctgtatcaaa agcactaacc ggaataattt tctgcatttt
     4501 tgtattactc ctggatataa gttttgataa atgctgattt tgcgatatgt atcagaataa
     4561 agatctggtt attggcatta aaacgatcga tatgtctgtc atattgagaa tttgcagatc
     4621 ttcgtcctat aatgggtgaa attaaatcag tgcacaagg
//
LOCUS       DAZGAP010000284         4633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGAP010000284 DAZGAP010000000
VERSION     DAZGAP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.286"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..287
                     /locus_tag="ACHLAM_004949"
     CDS             <1..287
                     /locus_tag="ACHLAM_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856338.1"
                     /translation="ALLFVFIPVYAHAAWYDPIVKFVKEFQTGIIIVGGAVAVGSLVY
                     VGVCWIISRMSGNMETTAMDYVKHIGVIGAVGGAVAAATWAYQLWGGTIS"
     gene            287..658
                     /locus_tag="ACHLAM_004950"
     CDS             287..658
                     /locus_tag="ACHLAM_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFO7856339.1"
                     /translation="MGETNEILEFDTNNAMNRVAMFAGIPVFALLFLVVIGCILTFSS
                     VWMWGWWGFIVTIPFILAIVGLRIICERDDKAFRRFRFQLRRWKMNRKYGRHLLLTSR
                     NPRWRDKYARRVIQKRILTGK"
     gene            615..3149
                     /locus_tag="ACHLAM_004951"
     CDS             615..3149
                     /locus_tag="ACHLAM_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFO7856340.1"
                     /translation="MQDALFKNAFSPESNAKVPWLGHHVAPEICTLGTSHILSLIRFS
                     GVPHDTRDQAMLNKEFMYLARCFKALGKQEGNNLKLQTYTFKRRIHLTHQYNLELPVL
                     QDLVDCWVKPFRNGTFRQVGYSMALILKFRDLDDGINRMRELLSLCREMLGDFGVSVM
                     ELEERDGKLYSQIGRFYSFILNGEEKDIPVTDTRLGDSIIDSETGFGAYDYVENRPSY
                     GKRRFASTYDLRDYPESKSYPGMWDEVLDEQYDFCMVQSFLFEDRNRMKRRLKTQMSD
                     LSSTEGQSSETEELADSVQEVTQGKKVFGEYYASLIVFGDTPEEAVDNGARMQSLLMA
                     RDTGFVRSRSTNYVTWLTLFPGYADVIYSMPKSTENLACGFSLHATPCGKADGNPPGD
                     GTALMPVMTENGGMYFLNAHASPLGKNCRGEKYPGHKTTIAMTGAGKTTVESLELVFF
                     SRWNPMIFSLDYNNSLENVLRALGTRYFNIKPGEFTWIQPFQMEDSVELRQYLFDVLV
                     QCAGGADSTEESKIQMSIDAVLKHKNVESRSFSLLLQSIPDTGPGGLRERLSKWCRDD
                     GNGRRGQYYWILDSPRNDFDPTLFRRLGFDCTKILNMEFATRHPQAMEVLLNTFFFLK
                     RRMHENEPGSLLINSIAEFWAPLMFESTANSIMEILHAGRTRGEMVMMDTQVPEYVLN
                     NERGPSVIQQTITQEWMANEKATSDSYSKFSVTSKEFSKIEEFSAYSRKVLVKQGGAS
                     VVLKVDMQDKLKYWLPLLSSDYANIPVAKRVREALGTDDPAVWVAPFLDEMVAIGVRK
                     ELAEKYPEQDVNDPQVWEPAFIEVMKQLERPVSMKLTTINNINSKS"
     gene            3218..3868
                     /locus_tag="ACHLAM_004952"
     CDS             3218..3868
                     /locus_tag="ACHLAM_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO7856341.1"
                     /translation="MIRKSCIAACLSITLCSLSYAGGVPTVSIAELTQLVVNAQQQAQ
                     EALAQLNKAKEAIRQAKDQYDHYKSILQGNDKLGAFLNDPYINKLLPMKDWQDIYNQT
                     QDLTDLRKRYGITGYEPDVQKLFDKLLAQVDVYEKQYDATVTRVNHAEELRNKLNSVQ
                     TPKDREQLALRYQQEMLELENQRMQLENTRYLMEQKERIENKKNEEDFYNFMWGRS"
     gene            3894..4292
                     /locus_tag="ACHLAM_004953"
     CDS             3894..4292
                     /locus_tag="ACHLAM_004953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856342.1"
                     /translation="MKIILKLCFLMLLLSGCNDKAKDFIGGWTQESNEEYPVYITINY
                     DSGIYHVDVKKLDTYLVEKKKADAFDDFMWGKKSKNESENLTFDDCYKVFNFEAKAIS
                     SSVLEGKGISVRMDGDRLIFDGKRFVRKKS"
     gene            4318..>4633
                     /locus_tag="ACHLAM_004954"
     CDS             4318..>4633
                     /locus_tag="ACHLAM_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7856343.1"
                     /translation="MQVNVAETLFRTIDTATQSQMANMNTVMSVMGIVIGGIWMTYIL
                     MKSLYWYFEGLTTVIQDAFFTIFKAFAIMMASLTISWYTSTVIPPVLEFPLWLANTIA
                     GVN"
BASE COUNT         1392 a          726 c         1109 g         1406 t
ORIGIN      
        1 ttgcgttatt atttgttttt attcctgtat atgctcatgc agcatggtat gatccaatag
       61 ttaaatttgt taaggagttt cagacaggaa ttattattgt cgggggagct gttgctgttg
      121 gttctcttgt ctacgtggga gtctgttgga ttatttctcg tatgtccggg aatatggaaa
      181 caacggcaat ggattatgtg aaacatattg gtgttattgg tgctgttggt ggcgctgttg
      241 ccgcagctac ctgggcgtac cagttgtggg gaggaacaat tagctgatgg gagagacaaa
      301 cgaaattctc gagtttgaca ctaataacgc gatgaacagg gtggctatgt ttgcagggat
      361 ccctgttttt gcattgcttt ttttggttgt tattggctgt attttgacat tttcttctgt
      421 atggatgtgg ggatggtggg gatttattgt caccatccct tttattctgg caatcgtagg
      481 attgcgtatt atttgtgaac gtgatgacaa agcgtttcgt cgttttcgtt ttcaattacg
      541 ccgctggaaa atgaatcgta aatatggtcg tcacttactg ttgacttcca gaaatccccg
      601 atggagagat aaatatgcaa gacgcgttat tcaaaaacgc attctcaccg gaaagtaatg
      661 ctaaggtgcc ctggttgggg caccatgttg cacctgaaat ttgtactctg ggcacgtctc
      721 atattctcag tttgatccgt ttttctggtg ttcctcatga tacgcgagat caggctatgc
      781 taaataaaga gtttatgtat cttgccagat gttttaaagc tttgggaaaa caggaaggga
      841 ataacctgaa attgcagacg tacactttta agcgtcgtat acatctcaca catcagtata
      901 atcttgaatt acctgttctt caggatttgg ttgattgctg ggtaaaacca tttcgtaatg
      961 ggacattccg gcaagttgga tattctatgg cccttatcct taagtttcgg gatctggacg
     1021 atggtattaa ccgaatgcgg gaattgttat ctttatgtag agagatgctg ggggattttg
     1081 gtgtttctgt tatggagctg gaggagcggg acggaaaact gtattcgcag attggccgct
     1141 tttactcgtt tattctgaac ggtgaagaga aagatattcc tgttactgac acccgcctgg
     1201 gagactcgat tattgacagt gaaacaggat ttggcgctta cgattatgtg gaaaaccgcc
     1261 cgtcgtatgg aaagcgccgg tttgcttcta cttatgatct aagggattat ccagaaagca
     1321 aatcctaccc aggaatgtgg gatgaagtgc tggatgaaca gtatgacttt tgtatggttc
     1381 aatcttttct ttttgaagat cgtaaccgaa tgaaacgtcg cctgaaaact cagatgtcgg
     1441 atttatcgtc tacggaagga caatccagtg aaactgaaga acttgctgat agtgttcagg
     1501 aagttaccca ggggaaaaaa gtttttggtg aatattatgc gagcctgatt gtatttggag
     1561 atacacctga agaagctgtt gataacggag caagaatgca atcgttgtta atggcgcgcg
     1621 ataccggctt tgttcgttcc cgaagtacaa attatgtaac ctggctgacg ctatttcctg
     1681 gttatgcgga tgtgatttat tcgatgccaa aatctacaga aaacttggcc tgtggatttt
     1741 ccctacacgc gactccgtgt gggaaagccg atgggaatcc tcctggagac ggaacagcgt
     1801 tgatgccagt gatgacggaa aacgggggaa tgtattttct gaatgcacac gccagtcctt
     1861 tagggaagaa ctgtagaggt gagaagtatc cagggcataa aacgacaatt gctatgactg
     1921 gggcaggtaa aacaacggtt gagagccttg aattagtgtt ttttagtcgc tggaatccta
     1981 tgatattttc tctggattac aataattcgc tggagaacgt actccgtgct cttggaaccc
     2041 gttattttaa tataaaacct ggtgaattta cctggataca accgtttcaa atggaagatt
     2101 cggttgaact acgtcaatat ctgtttgatg ttcttgttca gtgtgccgga ggtgctgaca
     2161 gcactgaaga atctaaaata cagatgtcga ttgatgctgt tctgaagcat aaaaatgtag
     2221 aaagtcgtag tttttcactg ttattacagt ctatacctga taccggtcct ggaggactca
     2281 gggaacgatt atcaaagtgg tgtcgtgatg atgggaatgg aagacgtgga cagtattact
     2341 ggatcttgga cagtcccaga aatgatttcg accctacgtt gtttcgccgt cttggatttg
     2401 attgtacaaa aattcttaac atggagtttg ctacacgtca tcctcaggca atggaagtat
     2461 tattaaatac tttctttttc ctgaagagaa gaatgcatga gaatgaacca ggcagcttac
     2521 taataaacag tatagcggaa ttttgggcac ctctaatgtt tgagagtaca gctaattcca
     2581 ttatggaaat tttacatgca gggcggaccc gtggtgagat ggttatgatg gatactcagg
     2641 ttcctgaata tgttctgaat aacgagcgtg gtccttcagt tattcagcag acaattacgc
     2701 aggaatggat ggcaaatgaa aaagctacct cagacagtta cagtaaattt tctgtaactt
     2761 ctaaagagtt cagtaaaata gaggagtttt cggcttattc acgtaaggtt ctggtaaaac
     2821 agggaggtgc atctgtcgtt ctgaaggtgg atatgcagga taagctgaaa tattggttgc
     2881 ctttattgtc atctgactat gccaatatac ctgtagctaa acgagttagg gaagcgttag
     2941 gtacagatga tccggctgta tgggtggccc catttctgga tgagatggtg gctattggag
     3001 taaggaaaga gcttgcggaa aagtatcctg aacaagatgt caatgatcct caagtatggg
     3061 agcctgcatt tattgaagtg atgaagcaac tggaacgtcc agtttctatg aagttgacaa
     3121 ccatcaataa tataaactca aagtcttgat tttatgatat caggaatgct ttgatgttaa
     3181 cgaaaacgtt cattcctgat aaacacagga gttctatatg atacgtaaat catgtatagc
     3241 cgcgtgtctc tctataacgt tatgttctct aagttatgca ggtggagtgc caacggtaag
     3301 cattgcagag ttaacgcaat tagttgtaaa tgcacagcaa caggcgcagg aagcgttggc
     3361 gcagcttaat aaagcaaagg aggcaattcg acaggcaaaa gatcagtacg atcattataa
     3421 atcgattctt cagggaaacg ataaattggg ggcatttctg aatgatccat atataaataa
     3481 attactgcca atgaaagatt ggcaggatat atataatcaa acccaagatt taactgattt
     3541 aagaaagcgt tatggtatta ccgggtatga acctgatgtt caaaaattgt ttgataagtt
     3601 acttgctcaa gttgatgtat atgaaaaaca atatgatgca acagtcacac gagtaaacca
     3661 tgctgaagag ttgagaaata aactaaattc agttcagact ccaaaggata gggagcagtt
     3721 agcattaaga tatcaacagg aaatgttaga gcttgaaaat cagagaatgc aactggaaaa
     3781 tacgcgctat cttatggagc aaaaagaacg aattgaaaat aaaaagaatg aggaggattt
     3841 ttataatttt atgtggggtc gctcataatc atattatata acgggaggaa tatatgaaaa
     3901 ttattttgaa attgtgcttt ttaatgttgc ttttgtctgg atgtaatgat aaagcaaaag
     3961 actttatagg agggtggact caggaatcaa atgaagaata tccagtatat ataaccataa
     4021 actatgatag cggaatttat catgtggatg ttaaaaaact agatacttat ctggtagaaa
     4081 agaaaaaagc tgatgcattt gatgatttca tgtgggggaa aaaaagtaag aatgaatctg
     4141 aaaatttaac atttgatgat tgttataaag tgttcaattt tgaagctaaa gcaataagta
     4201 gttcggtatt agaaggtaag ggtatatctg taaggatgga tggtgataga ttaatttttg
     4261 atggaaaaag atttgtaaga aaaaaatcat aatgtgacat agctaaagga tttttatatg
     4321 caggtaaatg tcgcggaaac gctgtttaga acaattgata ccgcgacaca atctcagatg
     4381 gcaaatatga atactgtcat gtctgttatg gggattgtga taggcggtat atggatgaca
     4441 tatatattaa tgaagtcatt atattggtat tttgagggct taacaacagt aatacaggac
     4501 gcatttttta caatatttaa agcatttgca atcatgatgg catcattgac tatatcatgg
     4561 tatacatcaa cagttattcc tccagtttta gaattccctt tatggttggc taatacaatt
     4621 gcaggagtta aca
//
LOCUS       DAZGAP010000285         4623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGAP010000285 DAZGAP010000000
VERSION     DAZGAP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.287"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            152..946
                     /gene="hyxR"
                     /locus_tag="ACHLAM_004955"
     CDS             152..946
                     /gene="hyxR"
                     /locus_tag="ACHLAM_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO7856344.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            1100..1861
                     /gene="hyxA"
                     /locus_tag="ACHLAM_004956"
     CDS             1100..1861
                     /gene="hyxA"
                     /locus_tag="ACHLAM_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO7856345.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     FLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVANAGTQALSLHKYK"
     gene            1903..4485
                     /locus_tag="ACHLAM_004957"
     CDS             1903..4485
                     /locus_tag="ACHLAM_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7856346.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADKAVSTDSVTLNKDKTT
                     LDQDVVINGDNKITAVTIETSDSDKDLNVTFGGHDITAASTVNQGFVEGVKVSGDKNV
                     VINATGSTITAQGEGTYVRSAMVISSTGDVVVNGGNFVAKNEKGSATGISLEGAQGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSVLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLKIKDGSVIGAANNKQTIYASASAQGKGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYAYAYAYAYAGVYADDDKASSNLNINLS
                     DGSVWKGNVAGSTTTGSTGTTENQSATVSLSDSTWKGNVGDGLDGGARVTLKNSSWTG
                     DVGNAKTAVFLTDGSVWNGAVSINAPVDRASVPMGSVQPNKSSVSLQNGSVWNVTGAS
                     TVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAGS
                     LDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSVI
                     QAMAAAPANVANLQADTLSARQDAVRLSENDEGGVWIQYFGGKQKHTTAGNASYDLDV
                     NGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGIF
                     IDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAKL
                     SALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFSD
                     GNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVGI
                     NVTW"
BASE COUNT         1277 a         1003 c         1157 g         1186 t
ORIGIN      
        1 cgcgttgtta aataacacaa aattaaaaac aataaaaatc aaacaaatta gatttatatt
       61 ccaataaaat taaatattca ggcaattaaa ccaaacctga cattttgaat caaaatgtca
      121 ggttttgtag caatggcgtg gacgagtctg aatgaagaag cctctggtta ttatcagtgc
      181 ctgtcagttc accaggctgg cactggagtc tctcatccca acggacaggt atatcgtgcg
      241 ggtgtacagc aacgtcacta cagaggttga attcgtttta aagacctcct gtggctacct
      301 gttagctgat atcccatcgc ttccttcggg agagttggtt cttctggctt gtcttgtccg
      361 cttgcggagt gatgccgggc aatggcaggt ctgcctgacc ggtgactccg actggttcga
      421 taatgctcct gctgcaagtc tgtataacgg cttcccacgc atcggcacca tgctgacagc
      481 gagtcgttat cagaatcaaa ttatccgctg gttactacgt cctttgcccg gcgatatgag
      541 cgatttctgg ctcctgactg cacgggagtt gagcgtgctt aagattctga tgcagggtat
      601 gagtttcagt gagattgccc gttatgaaca acgcagttca aagaccctgc atgcgattgc
      661 cacacgggct ttaatgaaac ttggactggg aacactgagc gatttcaggc tgctctatac
      721 gggatgcggt gacagcagag tgaaacggaa tatacggcta catgcaaaat atatcggaca
      781 acattccgct catcgactca ctcagtatgt gcagaacatg gtatccggaa ttctttatcc
      841 agagaaagtt gccgttggtg aatcaataaa ttctgacgcc atatccggaa aagtactttc
      901 cggacaaaga aaaaatatgt ctaaaaaaag accaggattg ttatgacaca gcagcgttgt
      961 tactaccccc cactgcggcg catacggact cgctacagtg taggagccaa aatatttcta
     1021 ctgctctggg ctgcaggatt gggaggcacg ctgtacagcc tgtacagcgt gctggcgtac
     1081 tgacatctgt gagggaagga tgcgtttcgt tcttttctgt ccctcaggaa ttgtaccagc
     1141 gcagttcgca gctctatcaa ccggttctgt cggcaatgtt acctgcatca gaacagaaga
     1201 ggagttacgc aataaattgc ggcacaggcc acagagtgtc gtgatatcgg cagggcgacc
     1261 tgccgaatgc gctgaaatgt ggttccgatt ttatcgggat cactcattcg tggtggtgtt
     1321 gtgcgtcgca cccttttttc tgccaccaga tgtgagtatt tctggcgttc tcaaaaacct
     1381 gaggttactg aaaccaggaa tgtcagtaga gcgtgtcatc agtattgcaa atgcctctgg
     1441 tgggttttca gggctgaaac atgcagaaat tttgccagtt atggacagtt actctgtctt
     1501 tatgaaagag gtgaataacc gcacaaagac aatcgtcatg tcagaacgct tcccggaaaa
     1561 gcaaaagaag gtgttgtcgt ttcttcttgc cggacacagc tgggaataca gtgcgcagtt
     1621 tctcaaaacg gggataagac agatatggct tgccgagcag tcgctaaaaa agcgttgggg
     1681 aataccggac agcatgtcat tgcgggaagc cctgctgctg agcagcaata attttcacgg
     1741 tgatggaaat gcattggaaa caacgaatgc catgacattg cgtgaaaacg gaaataccaa
     1801 caggtattcc gtggtggcaa atgcagggac gcaggcttta tctctgcata aatataaata
     1861 agtgggaaat tgaaattagt taccagaatg gagaatttct tcatgaaaaa cagtaaggca
     1921 ttttaccgca gcgcattagc aacagctatt gttatggctc tttctgcacc agcattcgct
     1981 gctgataaag cggtatcaac tgattcggtt acgctgaata aagataagac aactctggat
     2041 caagatgttg ttattaacgg tgataacaag attacagccg taacaattga aacgtcagat
     2101 tcagataaag accttaatgt tacttttggc ggtcacgata ttaccgccgc atcaacggta
     2161 aaccaaggtt tcgttgaagg tgtaaaagtt agcggtgaca aaaatgttgt gattaatgct
     2221 acaggctcca ccatcacagc tcaaggtgaa ggcacctatg tccggtctgc aatggtcatt
     2281 tcttcaactg gcgatgttgt tgttaatggc ggtaatttcg ttgcaaaaaa tgaaaaaggt
     2341 agtgcgacag ggatatctct ggaaggggcc cagggaaata atgtcacgct taatggcaca
     2401 accataaatg ctcaaggtaa taagagttcc agcaacgcct ccacggcaat ttttgctcaa
     2461 aagggtagcg tattgaatgg ttttaacggt gatgcaacag acaacattac ccttgctggc
     2521 tcaaatatta ttaatggtcg gattgaaaca atacttattg ctcaggagaa taagggaact
     2581 catacagtca acctgaagat taaggatggc tcagtaattg gggcggctaa taataaacaa
     2641 acaatttatg cctcagcttc ggcacaagga aaaggttcag caacgcaaaa tttaaatttg
     2701 tctgttgctg attcaaccat ctactctgat gtcctggccc tttctgaaag cgagaattca
     2761 gccgctacca caacaaatgt aaatatgaac gttgcccgct cttactggga aggtaatgct
     2821 tatgcttatg cttatgctta tgcttatgct ggtgtttatg ctgatgacga taaagcgagt
     2881 agtaatctga atataaatct ttccgatggc tcagtctgga aaggaaatgt tgcgggtagt
     2941 acgactactg gcagcaccgg tactacagaa aaccagtcag caaccgtaag tctgtctgat
     3001 tccacctgga aaggtaacgt gggtgacggt ctggatggtg gtgcgcgagt cacgctgaaa
     3061 aactccagct ggactggtga tgtcggcaac gccaaaactg ctgtttttct gacggatggt
     3121 tctgtgtgga atggtgctgt ttccattaac gcgccagtgg accgcgcatc agtaccaatg
     3181 ggctcggttc aaccaaataa aagcagtgta tctctgcaaa acgggtctgt ctggaatgtt
     3241 acgggagcct caactgttga tgctctggca gtaaaagaca gtacggttaa tatcacgaag
     3301 gctacagtca atactggcac gtttgcttct caaaacggca ctctgattgt tgatgcctct
     3361 tctgaaaaca ccctggatat cagcggtaaa gcgagcggtg acttgcgtgt ttacagtgcg
     3421 ggttcattgg atcttatcaa tgaacaaacg gcatttattt ctaccggcaa agacagcact
     3481 ctaaaagcca caggcacaac ggaaggtggt ctgtatcaat atgacctgac acagggagct
     3541 gatggtaact tttatttcgt aaaaaacacg cataaagcat ccaacgccag ctccgtgatt
     3601 caggcaatgg cagctgctcc ggctaacgtc gctaatctgc aggccgacac gctctccgcc
     3661 cgtcaggatg ctgtccgtct gagcgaaaat gacgagggtg gcgtatggat tcagtacttt
     3721 ggcggtaaac agaaacatac caccgcagga aatgcatcct atgacctgga tgtaaacggt
     3781 gtaatgctgg gtggtgatac ccgcttcatg actgaagatg gtagctggct ggccggtgtg
     3841 gcgatgtctt ctgcgaaagg tgacatgacc accatgcaga gcaaaggtga cactgaaggt
     3901 tacagcttcc acgcttacct gagccgccag tataacaacg gtatcttcat tgatactgct
     3961 gcacagtttg gtcattacag caacacggca gatgttcgcc tgatgaatgg tggcggtacc
     4021 atcaaagctg actttaacac caatggtttt ggtgcgatgg ttaaaggcgg ttacacatgg
     4081 aaagacggta atggcctgtt tattcagcca tatgccaaac tgtctgctct gacgctggaa
     4141 ggtgtggatt atcaactcaa cggcgtggac gttcattctg acagctataa ctctgtgctg
     4201 ggtgaggccg gtacgcgcgt gggttatgac ttcgctgtgg gcaactcgac cgttaaacct
     4261 tatctgaatc tggccgcact gaacgaattc tctgatggca acaaagtccg tctgggtgat
     4321 gagtctgtca atgccagcat tgacggtgca gcattccgcg tgggtgcagg tgtacaggct
     4381 gatatcacca aaaacatggg agcatatgca agccttgact acaccaaagg tgacgacatt
     4441 gagaatccgc tacagggtgt agttggtatc aatgtgacct ggtaagaata aagcagtcgg
     4501 ttcccctgtc gactgttata ccccctgcag gcagcagtat gctgcctgca ggctataaat
     4561 atccagtatt catgatgctg gatatttgct gatatttaac ccgaacattc ctgatacaga
     4621 cta
//
LOCUS       DAZGAP010000286         4603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGAP010000286 DAZGAP010000000
VERSION     DAZGAP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.288"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHLAM_004958"
     CDS             complement(<1..593)
                     /locus_tag="ACHLAM_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7856347.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHLAM_004959"
     tRNA            complement(708..783)
                     /locus_tag="ACHLAM_004959"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHLAM_004960"
     tRNA            complement(790..864)
                     /locus_tag="ACHLAM_004960"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHLAM_004961"
     tRNA            complement(981..1065)
                     /locus_tag="ACHLAM_004961"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHLAM_004962"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHLAM_004962"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHLAM_004963"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHLAM_004963"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO7856348.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGPRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHLAM_004964"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHLAM_004964"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO7856349.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHLAM_004965"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHLAM_004965"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO7856350.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1180 a         1158 c         1046 g         1219 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgcctg
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggccacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3901 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt accgcgatag tcttccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacggattg tccttttgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgcggggg gatggcttgt agatatgatg acaggaagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc ttc
//
LOCUS       DAZGAP010000287         4566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGAP010000287 DAZGAP010000000
VERSION     DAZGAP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.289"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(106..333)
                     /locus_tag="ACHLAM_004966"
     CDS             complement(106..333)
                     /locus_tag="ACHLAM_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856351.1"
                     /translation="MTRRGINAACGQCPASGRARDGRFYRSRLASVTVYASLSPFSDE
                     RPSSRFRGIALPSERRRLRYSTVGLTRYRTR"
     gene            234..440
                     /locus_tag="ACHLAM_004967"
     CDS             234..440
                     /locus_tag="ACHLAM_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856352.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKREAQRH
                     LLLLAKQNPKTTHKHPNKHQKQYK"
     gene            546..1508
                     /gene="parM"
                     /locus_tag="ACHLAM_004968"
                     /gene_synonym="stbA"
     CDS             546..1508
                     /gene="parM"
                     /locus_tag="ACHLAM_004968"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO7856353.1"
                     /translation="MLVFIDDGSTNIKLQWQENDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSDLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQKL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            1511..1861
                     /locus_tag="ACHLAM_004969"
     CDS             1511..1861
                     /locus_tag="ACHLAM_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7856354.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSTITQPSSQQEQR
                     SDEETKKNAMKLIN"
     gene            complement(2012..2524)
                     /locus_tag="ACHLAM_004970"
     CDS             complement(2012..2524)
                     /locus_tag="ACHLAM_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856355.1"
                     /translation="MNISVEDLFPESFQRIKEEEEANRPDPNWFAHLPPEKINAYMSK
                     YPFKSFDDIPLDNSGLQYPSLQELEFYFPPAALAANCHQLPERMRQKPVVTMINGLTL
                     LRSLGVQAFNIDPRRWHKIKTYLSQGTIEYPQMSEDGKVIIDGRHRILLIMQIYKVTD
                     VPVFFLKGIS"
     gene            2965..4500
                     /gene="cba"
                     /locus_tag="ACHLAM_004971"
     CDS             2965..4500
                     /gene="cba"
                     /locus_tag="ACHLAM_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin B"
                     /protein_id="HFO7856356.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRITLDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVRSNPEIQEDGVISETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISAVEILDSNSLSQRQEAENNAKDDFRVKKEQENDEKTVLTKTSEVIISVGD
                     KVGEYLGDKYKALSREIAENINNFQGKTIRSYDDAMSSINKLMANPSLKINATDKEAI
                     VNAWKAFNAEDMGNKFAALGKTFKVADYAIKANNIREKSIEGYQTGNWGPLMLEVESW
                     VISGMASAVALSLFSFTLGSALIAFGLSATVVGFVGVVIAGAIGAFIDDKFVDELNHK
                     IIK"
BASE COUNT         1403 a          951 c         1017 g         1195 t
ORIGIN      
        1 atgcagttac cgaggacaaa acgggacata acaacgctcc ttctcttaca gaggacacca
       61 ggagggcacc cctgtggtgc ccgttccccg ctgcctgtgg gctgttcacc gcgtccggta
      121 acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt tctgacggca aggctatgcc
      181 gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac aggctggcgt acactgtcac
      241 cgacgccagc cgggagcggt aaaagcgccc gtcccgtgcc cggccggacg cgggacactg
      301 accacaggca gcgtttatgc cgcgccgcgt cagccggcgc agtaaaaaga gaagcacagc
      361 gacatctttt gttactcgcc aaacaaaacc caaaaacaac ccacaaacac cccaataaac
      421 accaaaaaca atacaagtaa tcattgattg atagttgaaa tggggtaaac ttgacaaaca
      481 accccattta aaacccaaag cacacccaaa aacacaccac aaaaacacca taaggagttt
      541 tataaatgtt ggtattcatt gatgacggtt caacaaacat caaactacag tggcaggaaa
      601 acgacggaac aattaaacag cacattagcc cgaacagctt caaacgcgag tgggcagtct
      661 cttttggtga taaaaaggtc tttaactaca cactgaacgg cgagcagtat tcatttgatc
      721 caatcagccc ggatgctgta gtcacaacca atatcgcatg gcaatacagc gacgttaatg
      781 tcgttgcggt gcaccacgcc ttactgacca gtgatctacc ggtaagcgaa gtggatattg
      841 tttgcacact tcctctgaca gagtattacg acaggaataa ccaacccaat acggaaaata
      901 ttgagcgtaa aaaagcaaat ttccggaaaa aaattacatt aaatggtggg gatacattca
      961 caataaaaga tgtaaaagtc atgcctgaat ctataccggc aggttatgaa gttctacaaa
     1021 aactggatga gttagattct ttattaatta tagatctcgg ggggaccaca ttagatattt
     1081 ctcaggtaat gggtaaatta tcagggatca gcaaaatata cggagactca tctcttggtg
     1141 tatctctggt cacatctgca gtaaaagatg ccctttctct tgcgagaaca aaaggaagta
     1201 gctatcttgc tgacgatata attattcaca gaaaagataa caactatctg aagcaacgaa
     1261 ttaatgatga gaacaaaata tcaatagtca ccgaagcaat gaatgaagca cttcgtaaac
     1321 ttgagcaacg tgtattaaat acgctcaatg aattttctgg ttatactcat gttatggtta
     1381 taggtggtgg cgcagaatta atatgcgatg cagtaaaaaa acacacacag attcgtgatg
     1441 aacgtttttt caaaaccaat aactctcaat atgatttagt taacggtatg tatctcatag
     1501 gtaattaatg atggacaagc gcagaaccat tgccttcaaa ctaaatccag atgtaaatca
     1561 aacagataaa attgtttgtg atacactgga cagtatcccg caaggggaac gcagccgcct
     1621 taaccgggcc gcactgacgg caggtctggc cttatacaga caagacccca gggccccttt
     1681 ccttttatgt gaactgctga cgaaagaaac cacattttca gatatcgtga atatattgag
     1741 atcactattt ccaaaagaga tggctgattt taattcttca acaatcactc aaccctcttc
     1801 acaacaggag caaagaagtg atgaagagac aaaaaaaaac gcgatgaagt taataaactg
     1861 attaaatttt tattgatatc tttaccccca taaacggggt aaaggtatca acaaacctat
     1921 tttttagcta tgtacagatt acatctgaca agttatttct acgtaacaaa tcacagtcta
     1981 aaaatcagta tagctttacc ctttgcactg ttcagcttat tcctttcaga aaaaaaacgg
     2041 gaacatcagt tactttataa atctgcataa taagtagtat tctatgcctg ccatctataa
     2101 ttactttccc gtcttcggac atttgtggat attcaatcgt tccttgactt aaataggttt
     2161 taattttgtg ccatcggcgt ggatcgatat tgaaagcctg aacgccaagc gaccttaaca
     2221 gggttaaccc attaatcatc gtgaccacag gcttttgtct cattctctca ggaagttgat
     2281 ggcaattagc agccagggcc gccgggggaa aataaaactc cagttcctgt agtgaagggt
     2341 attgcaaccc actgttgtcc agaggaatat catcaaagga tttgaaagga tacttggaca
     2401 tatacgcatt gattttttca ggcggtaaat gtgcaaacca gttaggatca ggcctgttcg
     2461 cttcttcttc ttctttaatt cgttgaaatg actccggaaa caaatcttcc acacttatgt
     2521 tcatcacaat atcctccctt gagagcattg ctcatcgcca ccgataagga tttcattata
     2581 aaacacgcca gtacagaaca actcactggt ttcaaactcc cgtcgtccgt cctgaccggt
     2641 acggtaacta acaaggcctg tatacatatc acgatgtgta ccatgccata aagtgacagt
     2701 atccccccag gtgtctcata tcatagtttc attaaaacat gctgaggcca gagaacagtt
     2761 atgcctgtgc cgttttttgt ggtatcaggc gagaatagca cgtggtgtga atgcctgttt
     2821 tttgatcgtt ttcacaaaaa ttgaaatcca cagtcttgac agagaaaaca cagcggcgta
     2881 atttttgatc tgtacataaa accagtggtt atatgtacag tatttaattt taattgattg
     2941 tttttaaagt caaagaggtt ttctatgagt gataatgaag gtagtgtacc gacagaaggt
     3001 attgattacg gggacacaat ggttgtgtgg ccgtcaacag gacgaattcc gggcggtgat
     3061 gtgaaacccg gaggctcatc aggtctcgct ccatccatgc ctccgggatg gggggattac
     3121 agcccacaag gtatcgcact tgtacaaagt gttctttttc ctggaattat tcgccggatt
     3181 actcttgata aggaacttga agagggagac tggtcgggat ggtctgtcag tgtgcatagc
     3241 ccctggggaa acgagaaagt ttccgctgca cgaacagttc ttgaaaatgg tttacgtggt
     3301 ggtttgccag aaccgtctcg cccggctgct gtttcttttg cccgtctgga gcctgcttcc
     3361 ggaaatgagc aaaaaattat tcgtcttatg gttacacagc aactggagca ggtaacggat
     3421 atccctgcca gccagttacc agcagcgggt aataatgtac cggtaaaata tcgtctgacg
     3481 gaccttatgc agaatggtac acaatatatg gctattatcg gaggtattcc gatgacagtg
     3541 ccagtagtgg atgccgttcc agttccggac cggagtcgtc cgggaaccaa tattaaagat
     3601 gtttacagtg cccctgtatc accaaatcta ccggacctgg tattaagtgt gggtcagatg
     3661 aatactccag ttcggtctaa tcccgaaatc caggaagatg gcgttatttc tgagacaggg
     3721 aattatgttg aggctggtta tacgatgtcc agtaataatc atgatgtcat tgtccgtttt
     3781 cctgaaggca gtggagtttc tccgctatat atttcagccg tggagattct ggacagtaat
     3841 agtttaagcc agcgccagga agccgaaaat aacgcaaagg atgacttcag agtcaagaaa
     3901 gaacaagaaa atgatgagaa gacggtcctg acaaaaacca gcgaggtcat cattagtgtc
     3961 ggtgacaaag tcggggaata tcttggagat aaatacaagg cgctttcccg tgaaattgca
     4021 gagaatataa ataattttca gggaaaaacg attcgtagtt atgatgatgc aatgtcttcc
     4081 attaataagt taatggctaa ccccagcctt aaaataaatg caacggacaa agaagccatt
     4141 gtgaatgcgt ggaaagcatt taatgctgag gatatgggga ataaatttgc tgcgttgggt
     4201 aagacgttca aagtagcaga ttatgcaata aaggcaaaca acatcaggga gaagagtatt
     4261 gagggttacc agactggtaa ctgggggcca ttaatgctgg aagtcgagtc ctgggttatc
     4321 agtgggatgg catctgctgt agctcttagt ttgttttctt tcacattagg ctcggccctt
     4381 atagcctttg gtctttcggc cacagttgtt ggttttgttg gcgtagttat tgcaggtgct
     4441 attggtgcat ttatcgatga taaatttgtt gatgagttga atcacaagat cataaaataa
     4501 ttaaaaggaa ggcagtgtta ctgcctccct ttaaaatatt taatgacaag caatggcatc
     4561 agtaaa
//
LOCUS       DAZGAP010000288         4564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGAP010000288 DAZGAP010000000
VERSION     DAZGAP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.290"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(334..409)
                     /locus_tag="ACHLAM_004972"
     tRNA            complement(334..409)
                     /locus_tag="ACHLAM_004972"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(374..376),aa:Phe,seq:gaa)
     gene            complement(516..1091)
                     /gene="yjdC"
                     /locus_tag="ACHLAM_004973"
     CDS             complement(516..1091)
                     /gene="yjdC"
                     /locus_tag="ACHLAM_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7856357.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(1128..2825)
                     /gene="dsbD"
                     /locus_tag="ACHLAM_004974"
     CDS             complement(1128..2825)
                     /gene="dsbD"
                     /locus_tag="ACHLAM_004974"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO7856358.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(2801..3139)
                     /gene="cutA"
                     /locus_tag="ACHLAM_004975"
     CDS             complement(2801..3139)
                     /gene="cutA"
                     /locus_tag="ACHLAM_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO7856359.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(3255..4556)
                     /gene="dcuA"
                     /locus_tag="ACHLAM_004976"
     CDS             complement(3255..4556)
                     /gene="dcuA"
                     /locus_tag="ACHLAM_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO7856360.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
BASE COUNT         1165 a         1175 c         1223 g         1001 t
ORIGIN      
        1 cacaggagtc ttacaacgac catggggtat gttgatgtct gtatggagtc tgcagcacag
       61 acactggaag atgccctgag tttgtataca gatgagcgca cagtgagcaa gatttgcaaa
      121 ggggggaata aatcttgaca ggctgaatca gcgatgtaag catacagtgt taacgagaac
      181 agggcgtctc cgcaaaagac accctgtttg aggatacgat accgaactca atcagtgtaa
      241 ccacaactta cacaaacgga gaccctttga tgccgcatcc tgtattaacc gcccgtcaag
      301 gatctcatcc cttaaccagc gtgttctggt atatggtgcc cggaggcgga atcgaaccac
      361 cgacacgggg attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca
      421 ttaaaccgta atctgcacat ctcgtcaacc taatttcagt aaaagcgatt caactgctta
      481 agattgcggc aaatcgcttc ttttctgtgt ttctgtcagg tcaaagcacc accctggcgg
      541 cagcgggcga agcgcaggat atcttccgcc agccgatgtg cggtgtcgac atccgcctgg
      601 ctacgattca ccagcattcg gcttaaacag ccttccagca ccagttccat ctgctttgct
      661 accatcgccg gatcgtcaac ttccagtgtg gttaacagtt cgtgggtgaa atcgtaggcc
      721 gcgctttttt gctgatcggc cagttgatga atagggtggc caggatcggg ataaaacgta
      781 caggcagcga taaatagaca gcccggatag cggttgtttt taacgcactc cgataacgcc
      841 tgataacgtg ccagcagctt ttgttcggcg gtttgcgttt cgtccagcat cagctgacga
      901 cgccaggcat ctatctgttg gctaagataa cgcagcgcat cgtagaggat tgcctctttg
      961 tctggccaga agcggcgtag ctcgtccagt gggtaatcca cacgttcagc aaccatctca
     1021 agcgtggtgt tggcaatccc ttgtaattct aataatttca gggcttctcc cagtacatct
     1081 tcacgttgca cgctattttc ctccgtcttt cccactgcaa gtgtcgttca cggttggcga
     1141 tcgcgcaaat gtgcgctgaa ggtttcagca tccataaagc ccgtgacgcg tgcttgtgga
     1201 tgctcctggc cttgtccgtc aaaaaagaga attgtcggta ggccaaggac attaagatgc
     1261 tttaacagcg ccacatcttg tgcgtcgttg gcagttacgt tagcctgaag taagaccgtg
     1321 tctgctaacg ctttttgcac ctgcgggtcg ctgaaggtgt atttctcaaa ctctttacag
     1381 gcgacgcacc agtcggcata gagatctaac atcactggtt tgcctttggc ttcaacgagc
     1441 gcctgattta actcatctac cgttttgatt tgtgtaaagt tgagatgcgt ctgagtttgc
     1501 gcggtatgtg ttgcaccaaa tgcccaatcc tgaagtgggc gcacgctaac caatgccgct
     1561 gccagcagga ttatttgcac cacgcgcatc cagccgcgtt tggcctgtag gctggtgata
     1621 aaggcccagc caaagaatgc gacaccaagc gccgaccaca agcgtaatcc ccatatatcg
     1681 ccaatcactc gctccagcag gaagaccggc agtgcgagga tcacaaaacc aaacgcggtt
     1741 ttgacttgtt ccatccacgg gccgcttttc ggcagcaggc ggttaccaaa gacggttatt
     1801 agcatcagcg gcaggcccat gcccaacgca tagagataaa gcgtgccgcc ccccagccac
     1861 atgttcccgc tttgggcgat atacaacaga atcgcgctaa gcggtgcggt ggtgcatggt
     1921 gaacagatca gtccggcaat cgctcccata acaaacacac cgccaggtga gccgccctgt
     1981 tggcgattgc tcatcagcgt gaggcgcgtt tgcagcgaag aggggagttg cagggtaaac
     2041 aaaccaaaca ttgacatcgc cagcaaggta aagacgatgg cgaggccaat gagcacgtat
     2101 gggtgctgta gcgccgcctg gaactgtaac cctgcggcgg caactaccag acccagcgcc
     2161 gtgtaagtca gtgccatccc ctgcacataa ataaaggtca gcaacaatgc tctggcagtg
     2221 gagagccgct gtttaccacc cagcacgata ccagaaatca gtgggtacat tggcagcacg
     2281 catggcgtaa aggcgatacc aataccgatc aacaacgccc agagcgcgga aaagggcaat
     2341 tgcgcggtgg gctgctcttg ctgcggaaca gacacaggct gtgacgctgc gttgttggcg
     2401 accacttcgc ttaacggaac ggttttggtt tctggcggat aacagaaacc ggcatcagca
     2461 cagccctggt aggtgacagt taacgtcgct cccgcactcg cctggttgat ggttacggga
     2521 agcgtcagcc gatcgcggta aatctcgctt ttgccgtaaa actcatcttc atgccagacg
     2581 ccttgtggca gctgcacgtt ggcaattttc gcgtgttccg gcgtaatgcg gatctgttta
     2641 cggtagaggt aataaccgtc tttgatctgc caggtcagat tcaggtcatg ttggttttgc
     2701 tgaaaatcaa aagcaaaggc ttgatccgcg gggacaaatt gtgaacgtcc cggcgcgtcg
     2761 aataatccgg caaaaacgga agtgctgcaa agtagcagga tcagcgtaaa gatgcgttga
     2821 gccatgagag gtaatctgtg tctccgtgtg taacaggtaa aaccagaagt tccggggttt
     2881 gatatggatg atgagacttc aggcattcca gcagtgcctg ctggtgagat acggtagttt
     2941 ttaaaatcat ctgcacttcg tattcttgct ccagcttacc ttcccagtaa tagagagaag
     3001 tagcgccggg gatcaaggtc gcgcaggccg ccagtttttc cgccagcact ttggcggcta
     3061 aatcctgggc tgtcgcttca tccggtgcgg tacatagcac cacgacagac gcggtattcg
     3121 aacttttttc atcaagcata aacacctcgc aagaacagat ggaaccgcaa gagagaggtc
     3181 actatacaac ggacggggga aggatgttag tcggcgggaa agaaagcggg gcgtgaacgc
     3241 cccgcgattg atcattacag catgaagcta cccagcacga agccgaagca aacggccagg
     3301 gcaacaccca gagtacccgg gatgaagaac ggatggttga agacgaattt accgatacgg
     3361 gtagtacccg tgtcatccat ctgtaccgca gcaaccagcg tcgggtaggt cggcagaatg
     3421 aacagaccag acaccgcagc gaaagaagca acagcggtca gcggagaaac gttcagtgcc
     3481 agagccatcg gcatcagtgc ttttgcggtt gcagcctgag agtacagcag agcagaagca
     3541 aagaagaaga tgacggccag cagccacgga tgaccctgaa tcacttcacc agcggtatct
     3601 ttgatccagt cgatgttgtt ggaaacgaaa gtatcgccca gccacgcaac acccaggata
     3661 caaatacagg cgctcatacc tgctttgaag gtgctggagt tgaggatgtt gtcggtatcg
     3721 actttacaga taacggtggt cagagttgca acgctgagca tgatgatcag gattgcgttg
     3781 gtggtgttca tcagcggttt ttcaaccaga cccatgcttg ggctgttgat gattgcatag
     3841 ataacaacgc caactacgcc cagcaggaac agccagacgg acgtttttgc accggatttg
     3901 atttcaatct gcttttcacc gcgcagttca accaggcctt cttccagacg cttgcgataa
     3961 atcggatcgt cagagagttt ggagttgaac agcatagtga ccaggaagga catcaccaga
     4021 accgccagca gggtggacgg gatgaccacg gagagcagat ggaggtagct gatgccatga
     4081 ccttccatta cggaagacat gtaaaccact gccgctgaga ttggcgatgc ggtgatcgca
     4141 atctgcgcag ataccactgc agtagacagc ggacggcaag gtttaacgcc ttgttccttc
     4201 gcaacttcag cgataactgg cagtgtcgcc agagagatgt tgccagtacc agcaaagatt
     4261 gtcaggaaat aggtcacgat cggtgcgagg atcgtgatgt atttcgggtt acggcgcagc
     4321 agcttttctg tctgatgaac cagatagtcc agaccgccag caacctgcat ggcagaaata
     4381 gcggcgataa ccgccatgat aatggaaatg acatcgaacg ggatgttacc gggtttaacg
     4441 ccaatagcgg caagaaccag cacccccaat ccgcctgcaa aaccaatacc tattcccccc
     4501 aatctggcgc ccaagaagat cgccagcaaa actatgatga gttctacaac tagcatatta
     4561 gcct
//
LOCUS       DAZGAP010000289         4560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGAP010000289 DAZGAP010000000
VERSION     DAZGAP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.291"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            698..868
                     /locus_tag="ACHLAM_004977"
                     /pseudo
     CDS             698..868
                     /locus_tag="ACHLAM_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1084..1917)
                     /locus_tag="ACHLAM_004978"
     CDS             complement(1084..1917)
                     /locus_tag="ACHLAM_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7856361.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(1924..3261)
                     /locus_tag="ACHLAM_004979"
     CDS             complement(1924..3261)
                     /locus_tag="ACHLAM_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFO7856362.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPPSGASTLVWRQE"
     gene            complement(3359..4369)
                     /gene="iolG1"
                     /locus_tag="ACHLAM_004980"
     CDS             complement(3359..4369)
                     /gene="iolG1"
                     /locus_tag="ACHLAM_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFO7856363.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(4387..>4560)
                     /locus_tag="ACHLAM_004981"
     CDS             complement(4387..>4560)
                     /locus_tag="ACHLAM_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFO7856364.1"
                     /translation="VPGDGNIDFKSVLAYLVENHYSGWIVVEAEQDPNIYNPLEYAQK
                     GKKHIDGLLGNYI"
BASE COUNT         1293 a         1154 c          890 g         1223 t
ORIGIN      
        1 tttaactggt tgctgatata agtttttcac cacgcataaa cactgcccat gcgatcctgg
       61 caatcttatt tgccagctct atgatgacga tattcgttgg tcgacgtgcc tgctgggctt
      121 aaatctattc ccccggggat ccggctttat tgcaaccata aagctgcgag cccctatgta
      181 attcgctgac tcacacctca tttactgcgc gggattcaag cccaaaaccc catttaggtt
      241 tagtgagtcc ctgtcttacc tgtctttgtc atcacgttga ccaccagagc aacctttctg
      301 cattcaccca tttaagtaac caaaatgagt atcaggcctg atatatacag aactgaattc
      361 gttaattaat tattaatcag ttaaattttc cgttactggg atgaggtgta cacttggcct
      421 ctcccaaaat ccgcggaacc gcccctggcc cctattcagc atttcaggct ctaagttgag
      481 gactgaccgg attgacatag ggcctgctga cgccattcat tgactaccaa cagatctttc
      541 ttgttattta tagaaaacca ccagccagcc tagtggttca gtaaaacttc caactttgag
      601 gctgttgtca aaaccccttt ttgatttgtt aaaacacctt gcagtctacc aactgtaagc
      661 gtcaaacaag aaatcaaaag gggattccaa tgggtgaatg aaaaagggct taacgaacac
      721 ccgatggaac tgtaaatatc acatagtttt tgcgccaaaa taccgaagac agatgttcta
      781 cagagaaaag cgaagtgcaa gagcaccagg caatattttg agaaaactgt gtgagtggaa
      841 aagtgtacgg attctggaag ctgaatgctg agcggatcat atccatatgc ttgtgaattt
      901 acggggcgta agtaaggaag ttgaatgaaa atgtcagatc atatgcgcct gtcagccagg
      961 gcgaggcaga aaacgaatct ttaagacaca tctgaaaaac ctccggctat gccggaggag
     1021 atttattctg atcacctgct tattaccggg cacagcgaca gttcatttgc agttacgtca
     1081 gcgttaatcc gctaagaaag gtcgcgtttt ttccgctccg atagcaaaga cctcgctggc
     1141 tgccatttgc cagtaacctg ggttaaacaa ctccagtgaa cagatccctg tataaccttt
     1201 tttcaccagt tcatgggtga tcatgtacag tggtatcgca ccgttgcctg gaaacagacg
     1261 atgacaatga tccaacacag ctacaggtaa acggtcagca tcatcaatat gatagacaaa
     1321 gatcttttcc accggaacct gtcgcagcgt actcagatcc tgccattgat cgtacagata
     1381 aaggttaaag gcatctattg ccagaccaac gttactccga tcaactgcgt ccacgatctc
     1441 catcgccatc gccagactgc gtacgcaaca tcccgcagag ccgataggct caaaggccag
     1501 tcgcatctgg ctctctgccg ctaatgctga catctcacgc aaacgtttca ctgaatctct
     1561 taccgtgtca cgatctgaaa aatgagcacc ttcacgaatg gtcggcacta caaccaggca
     1621 atcaccacct acgacttccc ctgcataaca ggctaaaacc agttgttgcc gtatttccga
     1681 ccagcgcccg gcatcacaaa atgtgatgtc ttccagcgag ttgtagcccc atggcttaag
     1741 atggcttaca gaaaaaatat cggccagttc aaccagagtg tgttcctgca gccacgtctg
     1801 caacatatcc agacggatct caatatagtc atatccaaaa cgttccgcta ggaccagatc
     1861 cttctccagg ctggaattac gcagacaggt cgcttcattg aatcccagtt tcatcattca
     1921 gacttactcc tgccgccata ccaatgtact ggctccagaa ggcggcagcg ttaatgcgag
     1981 ttctctgtcg ccatcgacca cccggaaatg cctttctcgc gtatcgcgat tatataccac
     2041 taaaactcgc tcaccatcag gattaacaaa acccacatct tccagttgcg tatcataact
     2101 ggaagtaagc ataactttcg caccgggatg cacaaagcgg caaaagtgtc caattccgta
     2161 ccaagagtgg ttacaccaga gaatgtcatt caatgcatcg tactgcacag gagcttcgca
     2221 caaattactc tggtgatttg ggcctccatc actgtctaac agcaggttcc agtcgataaa
     2281 tccgctacag ccggatttaa aattaccaat catgtcatga atataggtat gccagtgacg
     2341 gatctggcca ccttcatcat tctccattgg tacacatcct tcggaaaaca gcagcttttt
     2401 attcggcaac ttccgggcca gatactgaat ttgcgaaaaa tggtcaccgg tataccagtg
     2461 gaaagccatc ccgttgatgc tatcgccatc cttttcgtca gcaaaggcac gttcagccca
     2521 gtcaatcaga ccatctttat catgatccca gatatagatc tctatttcat tcattccata
     2581 gcgggcgagt cgtgggcgca gatagtgaac agcaaaagta gtctcttctt ctacactata
     2641 cagacaggaa tcccagcttt ttatcgcaac tggttcattc tgcactgata gtgctttaac
     2701 actgatacca tggcgtctgt attcgagcag atagtcgata ataatatctg cccagtcagc
     2761 atagtattca taccgcaatt tcccacctcc gttcatatca taattagtct tcatgaatgc
     2821 cggtggactc cacggggaag ccattaattt tatttgtgga ttgatccgca atgccgcgga
     2881 aattagcggc attaaatgag attcatcacg ggaaaaggaa atccttccct gacgcaggtc
     2941 ttcactggaa tcaacatagg cataattgct cagagagaaa tcacagcttt ggatcggcat
     3001 ccgccccaaa ctatagttat gttcctgagg cgaaaaatag agattcaaaa attgcgcctt
     3061 tgtcttatca ctcatgctgt tgaaaacaac accagcactt tcagtgaagc tgccgccaaa
     3121 tccatcaatc tgttgtaacg agtggtttgg aaagacgctg attaattcac tacacgctgg
     3181 ttgacgaggc gaaaaaaaaa cctccagctc aaccataaat tgctgattcc agggatcgga
     3241 agtgattagt ctgcctttca tagagggatt ccagataata taggatgata aattgtgata
     3301 taccggacag gagcatacac ctagctatcc ggtatgaaat tatcacatcg cagaaagact
     3361 atttgtagaa gtcaggtttt gctggtaatt ccacaaactc ggtgtttcct gtttcctggg
     3421 acttcacaca ggcatcagcc gttaccgcag ccagataacc atcccatgat gttgggcctg
     3481 ccagaggtaa tcccgcattc agacggtcaa agaagtcctg gaactcaata tcataggcat
     3541 caataaagcg ttgtttccag tccaccaaaa tgtcagtact gtattttgcc tctttacgca
     3601 ccgcagcagt agccacggta ggtaattcca ccatcccttt ctcacaggtc acatcacaat
     3661 gaatatcata gccatactgg caattcacaa acacctcaac cactatatta ataccggagg
     3721 tcgtttccat caccaccacc tgcggatctc gtagagtagt caccaagctg gactgacgtg
     3781 ggaagtagac cttaactgtt ctataatctt cgttgagtaa ccagtgcata acatcgattt
     3841 catgaatcag cgtttcataa attgcttgtg gtgttttgta atccgatacc gtgcttacat
     3901 tataatgacg accatgaacc attaatggtt gaccaatttc tccgctatcg atgatgtttt
     3961 tcagctgtac gtagccttta tcgtagcgac gcataaaccc aacttgcacc attcgttttc
     4021 catttttacg ttcagctccg atcacacgct gacaatcatc tgctgtaacc gcaagcggct
     4081 tttcacagaa aacatattta ttggcattta acgctgcaac ggctacatcg gcatgtactt
     4141 cattcgaggc agtaattacc actacttcga catcttcatc attaatcaga tcgtgatagt
     4201 ctttatagtc ttttgctttg atctcatact tatccaaagc tgcttgcgct ctgccttcga
     4261 caatatcgca cacagcaaca acttctactc cagaaatagt gttagccaga cgtcttaagt
     4321 gatcggcacc aattttcccg atccccacaa tacctgcttt taaagtcatc ttattttcct
     4381 tttctatcag atataattac ccaataaacc atcaatatgc tttttacctt tttgtgcata
     4441 ttccaaagga ttatatatat tcgggtcctg ctcagcttca acgacaatcc agccggaata
     4501 atgattctca accaaataag ccaatacaga tttaaaatca atattcccat caccaggaac
//
LOCUS       DAZGAP010000290         4518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGAP010000290 DAZGAP010000000
VERSION     DAZGAP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.292"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(13..333)
                     /gene="chbB"
                     /locus_tag="ACHLAM_004982"
     CDS             complement(13..333)
                     /gene="chbB"
                     /locus_tag="ACHLAM_004982"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO7856365.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(632..970)
                     /gene="osmE"
                     /locus_tag="ACHLAM_004983"
     CDS             complement(632..970)
                     /gene="osmE"
                     /locus_tag="ACHLAM_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO7856366.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1172..1999
                     /gene="nadE"
                     /locus_tag="ACHLAM_004984"
     CDS             1172..1999
                     /gene="nadE"
                     /locus_tag="ACHLAM_004984"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO7856367.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2229..3116
                     /gene="cho"
                     /locus_tag="ACHLAM_004985"
     CDS             2229..3116
                     /gene="cho"
                     /locus_tag="ACHLAM_004985"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO7856368.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3076..3651)
                     /gene="ves"
                     /locus_tag="ACHLAM_004986"
     CDS             complement(3076..3651)
                     /gene="ves"
                     /locus_tag="ACHLAM_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO7856369.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3854..4339)
                     /gene="spy"
                     /locus_tag="ACHLAM_004987"
     CDS             complement(3854..4339)
                     /gene="spy"
                     /locus_tag="ACHLAM_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO7856370.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1129 a         1097 c         1075 g         1217 t
ORIGIN      
        1 atttaaaata aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac
       61 gcctaaacca tcgactttgc cataaagcag cgagtcaatt acttcgaccg gtttgttggg
      121 taacaaacgc tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc
      181 ggcattctga cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac
      241 ttcatatttt tctgcctgtg cgcgcatttt tgataccagt aaagaggtag acatgcccgc
      301 agaacaaaac agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg
      361 tcagccagac actccgcaag ccttaacctg cttccatgct ctgggtaact tgcgaaacca
      421 acatgacttt ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct
      481 ttgacctgat aaattgtctc tccttgacct gcggttatga ccctcttcac atttcgggca
      541 aataattcgc gaagataatt aagaactcag gccaaaaaaa accggcgcaa tggccggttt
      601 ccgttgtaac tcaagctttc aggcgaagtg attacttcgc agcctgtgga tcagtgtcgt
      661 attcagcaca ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa
      721 agtaggtttc tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccacgag
      781 catggatcat gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc
      841 tcatgccttt tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat
      901 aagccgtaca acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat
      961 tcttgttcat attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg
     1021 gcgctttttc aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt
     1081 ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg
     1141 aagttttgtc ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc
     1201 gctgggcgca aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct
     1261 gaaaagctac ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg
     1321 tcaggactcc acgcttgccg gaaagctgtg ccagatggcg attaatgagc tgcgccagga
     1381 aactggcaac gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggctga
     1441 cgaacaagat tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa
     1501 tatcaagggc gcggtattgg ccagcgaaca ggcgctgcgc gaagcgggta ttgaattaag
     1561 tgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc
     1621 gggtatgacc agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt
     1681 cttcactaaa tatggtgacg gcggtacgga cattaatcca ctgtatcgtc ttaacaaacg
     1741 tcagggtaaa cagttactgg cggcattagg ttgcccggaa cacctttata agaaagcgcc
     1801 aacggccgat ctggaagatg atcgcccttc tctaccagat gaagtggcac tcggcgtgac
     1861 ctatgacaat atcgacgact atctggaagg gaaaaacgta cctgaacagg tcgccagaac
     1921 aatagagaac tggtatctga aaactgaaca taaacgccgt ccgccaatta ccgttttcga
     1981 tgatttctgg aagaagtaat aatttgcaca tattggtttg tgcgaaaaag agtactttgt
     2041 tcacgccgga tgcggcgtga acgccttatt cgccccacaa aactatgcaa attcaatata
     2101 ttgcatgagt cgaataggcc tgacaggcgt agcacgtctg acggtgtaac ctttgtcatc
     2161 aacccgcctc ttttttaatc gctccccgcc tgttacactg gatagataac cagcattcgg
     2221 agtcaactgt ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt
     2281 atgaatatcc cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt
     2341 atctgtttca tggcgaaagt gacaccatgc cgctctatat cggcaaaagc attaacattc
     2401 gcagccgcgt cctttctcat ttacgtaccc cggatgaagc cgccatgcta cggcaatccc
     2461 gacgcatcag ctggatatgt accgccggtg aaatcggcgc tctactcctt gaagcgcgat
     2521 taatcaaaga gcagcagccg ctgtttaata aacgcttgcg ccgcaatcgc cagctctgtg
     2581 ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac
     2641 gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga
     2701 ccatcgctga tgaacaaaaa ctttgttatg gtctgttggg actggagccg ttaagtcgcg
     2761 gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga
     2821 gccatgacga tcatgcgcta cgtttgcgcc agtctctgga acgtttacga gtagtttgtt
     2881 ggccgtggaa aggtgcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata
     2941 ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg acaatgttaa
     3001 ttcggacacc cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt
     3061 ccggtaacta tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc
     3121 actgaacaac agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca
     3181 gcacgcgccc tggtcggtag tgagcagttt atcgcccaat tgccacgccc cgttaatgac
     3241 aaacactacc ccgccacgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt
     3301 cgccttacag acatccaggc gtgtcatgat gttgaaatcc atcgacatct gccctgccgt
     3361 cagtttcgct tttaccacct ggtccgccgc aaaggcaaaa ggctgtaacg gttttaaggt
     3421 atggttaaag cggtctgtgc tttcaaggaa catctcgccg ccttccagca acgtcactat
     3481 cctttccata cctggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc
     3541 acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc
     3601 agcatttcgc cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc
     3661 cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg
     3721 tgatttacgt tagtggcgat caggcttttt ttgcatctgt ttgagaacaa aaaagaaaac
     3781 cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttacgttt
     3841 tttggctgaa agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg
     3901 tcagacgctt ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aagatgttgt
     3961 aaatcttgtt ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt
     4021 ttgcgatctg cgcttcagct tttgctttat cgaaggtatc gctggcaatg atgtcatgca
     4081 ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt
     4141 cgcggatctg ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat
     4201 gcggaccgaa cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgaggtag
     4261 tggtgtctgc ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca
     4321 gtgcagttaa tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt
     4381 aagtagcgtg ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc
     4441 aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa
     4501 aatttgatgg agagtaat
//
LOCUS       DAZGAP010000291         4397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGAP010000291 DAZGAP010000000
VERSION     DAZGAP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.293"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            150..1307
                     /gene="acrE"
                     /locus_tag="ACHLAM_004988"
     CDS             150..1307
                     /gene="acrE"
                     /locus_tag="ACHLAM_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO7856371.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1319..>4397
                     /gene="acrF"
                     /locus_tag="ACHLAM_004989"
     CDS             1319..>4397
                     /gene="acrF"
                     /locus_tag="ACHLAM_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO7856372.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFV"
BASE COUNT         1062 a         1073 c         1168 g         1094 t
ORIGIN      
        1 ttatgtgcct tgagacgcca gtattcataa ctattcctta catcgacgaa tgataatttg
       61 taggatagcg aactgtattt ttctttctgc gagttaacgc gttgcctttt tgggtaaata
      121 acgcgctttt ggttttttga ggaatagtaa tgacgaaaca tgccaggttt ttcctcctgc
      181 cctcctttat tctgatctcc gcggctttaa tcgccggttg taacgataag ggagaagaga
      241 aagctcacgt cggtgaaccg caggttaccg ttcatattgt gaaaacggcc ccgttagaag
      301 ttaagactga attaccaggc cgcaccaatg cttaccgtat agccgaagtt cgcccacagg
      361 ttagcgggat cgtactgaat cgcaatttca ctgaaggcag cgatgtgcaa gcaggccagt
      421 ccctgtacca gatcgatccc gcgacctatc aggcaagtta tgacagcgcg aaaggcgaac
      481 tagcgaaaag tgaagccgcc gccgccatcg cgcatttgac ggtaaaacgt tacgttccgc
      541 tcgtgggtac gaaatacatc agtcagcagg agtacgacca ggccattgct gatgctcgcc
      601 aggccgatgc cgccgtgatt gccgcaaaag ctacagttga aagcgctcgc atcaatctcg
      661 cttataccaa agtgactgcg cctattagcg gacgtatcgg caaatcgact gtgaccgaag
      721 gcgctcttgt cactaatggg caaacgactg aactggcgac tgtccagcag ctcgatccta
      781 tctacgttga tgtgacccaa tccagcaacg attttatgag gctgaagcaa tccgtagagc
      841 aaggaaattt gcataaggaa aacgccacca gcaacgtaga gttggtcatg gagaacggtc
      901 aaacctatcc cctgaaaggt acgctgcaat tctccgatgt gaccgttgat gaaagcaccg
      961 gctccataac cctacgtgct gtcttcccta acccgcaaca tacgcttttg ccgggtatgt
     1021 ttgtgcgcgc acggattgat gaaggcgtcc aacctgacgc cattcttatc ccgcaacaag
     1081 gcgttagccg cacaccacgt ggtgatgcaa ccgtgctgat tgttaacaat aaaagtcagg
     1141 ttgaagcgcg ccctgtcgtt gccagccagg cgattggcga taaatggttg attagcgaag
     1201 ggctgaaatc tggcgatcaa gtcattgtca gcggcctaca aaaagcgcgt ccgggagagc
     1261 aggttaaagc cactaccgat acccccgcag atactgcatc gaagtaaggc aatctgacat
     1321 ggcaaacttt tttattcgac gaccgatatt tgcatgggtg ctggccatta ttctgatgat
     1381 ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag tatccaacca ttgcaccgcc
     1441 tgcggtttct gtttcagcaa actatccggg cgctgatgcg cagaccgtgc aggatacggt
     1501 gacgcaggtt atcgaacaga atatgaacgg tatcgataac ctgatgtata tgtcctccac
     1561 cagcgattcc gccggtagcg tgacaattac ccttaccttc cagtccggga ccgatcctga
     1621 tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc acgccgttgc tgccgcagga
     1681 agtacagcag caggggatca gtgttgaaaa gtccagtagc agctatttga tggtggcggg
     1741 ctttgtctct gataacccag gcaccacaca ggacgatatc tcggactatg tggcctctaa
     1801 cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac gtacagcttt tcggcgcaca
     1861 gtatgcgatg cgtatctggc tggacgccga tctgctaaac aaatataaac tgacaccggt
     1921 tgatgtgatt aaccagttga aggtacagaa cgatcagatc gctgccggac agttgggcgg
     1981 aacgccagcg ttaccagggc aacagttgaa cgcctcgatt attgctcaga cgcggttaaa
     2041 aaatccggaa gaattcggca aagtgaccct gcgcgtaaac agtgacggct cggtggtacg
     2101 cctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac tacaacgtta tcgctcgtat
     2161 caacggaaaa ccggcggcgg gcctggggat taagctggca accggcgcaa atgctctcga
     2221 taccgcgaaa gccattaagg caaaactggc ggaattacag ccattcttcc cgcagggaat
     2281 gaaggttctc tacccttatg acaccacacc attcgtccag ctttctattc acgaagtggt
     2341 aaaaacgctg ttcgaagcca ttatgctggt gttcctggtg atgtatctgt tcttgcagaa
     2401 tatgcgagca acgctgatcc ccaccattgc ggtacccgtg gtgttgttag ggacgtttgc
     2461 catcctcgcc gcttttggtt actccatcaa cacactaacg atgttcggga tggtgcttgc
     2521 catcgggctg ctcgtcgatg atgcgatagt ggtggtggag aacgtcgagc gcgtgatgat
     2581 ggaggataag ctcccgccaa aagaagcgac ggaaaaatca atgtcgcaaa tccagggcgc
     2641 actggtgggt atcgcaatgg tgctgtcagc ggtatttatt ccgatggcat tcttcggcgg
     2701 ttctactggg gcaatttatc gtcagttctc tatcaccatc gtttcggcaa tggcgctttc
     2761 tgttctggtg gcattgattc ttacccctgc gttatgtgca acgctgctta aacccgtctc
     2821 tgctgagcat cacgaaaata agggcggttt cttcggctgg tttaatacca ccttcgatca
     2881 tagcgttaac cactacacca acagcgtcgg caaaatcctc ggttccacag gacgatattt
     2941 actgatctat gcactaattg ttgcaggaat ggtggtgttg tttttacgtc ttccgtcttc
     3001 cttcttacct gaagaggatc agggtgtctt tctgactatg attcagttac ccgctggcgc
     3061 gacgcaagag cggacgcaaa aagtactgga tcaagtgacg gattactatc tgaagaacga
     3121 gaaagcgaac gttgaaagtg tctttacagt taacggcttt agcttcagcg gccaggcaca
     3181 aaacgccggt atggctttcg tcagtctgaa accgtgggaa gagcgtagtg gtgacgaaaa
     3241 cagtgcggaa gcggtaatcc atcgtgccaa aatggaattg ggcaagatcc gcgacggttt
     3301 tgtcattcca ttcaatatgc cagccattgt tgaactgggc acggcaacgg gtttcgactt
     3361 tgagttaatt gatcaggctg ggctgggtca tgatgccctg acccaggccc gtaaccagtt
     3421 gcttggcatg gcggcgcaac atcctgccag cttagtcagc gtgcgtccta atggtctgga
     3481 agacaccgcg cagtttaaac tggaagttga ccaagaaaag gcgcaggcat taggtgtttc
     3541 actttctgac atcaatcaga ccatttcaac agcgttgggt gggacttacg ttaacgactt
     3601 cattgaccgt ggtcgcgtaa aaaaggtgta tgttcaggcg gatgccaaat ttcgtatgct
     3661 gccagaagat gtcgataaac tttatgtccg tagcgccaat ggcgaaatgg tgccattctc
     3721 tgcctttacc actgcacatt gggtgtatgg ttctccgcga ctggagcgct acaacggtct
     3781 gccgtcaatg gagattcagg gggaagccgc gccaggaacc agttccggcg atgccatggc
     3841 gttgatggaa aaccttgcat cacaattacc agctggtatt ggttatgact ggacgggtat
     3901 gtcatatcag gaacgcttat cgggaaacca ggctcccgct ctggtagcaa tttcctttgt
     3961 ggttgttttc ctgtgccttg ctgcactcta tgaaagctgg tcaattcctg tctcggttat
     4021 gttggtcgtg ccgttaggga ttgtcggcgt gctactggcg gcgacactct ttaatcaaaa
     4081 aaatgacgtc tactttatgg tgggcttgct aacgacaatt ggcttgtcgg ctaaaaacgc
     4141 tatattgatt gttgagttcg ctaaagatct catggagaaa gagggtaaag gtgttgttga
     4201 agcgacacta atggcagtac gtatgcgtct gcgtcctatc ctgatgacct ctctcgcctt
     4261 tattctcggc gtattaccgc tagctatcag taacggtgcc ggcagtggcg cgcagaacgc
     4321 agtgggtatc ggggtaatgg gcgggatggt ttctgcaacg ttgctggcaa tcttcttcgt
     4381 gccggtgttc tttgtgg
//
LOCUS       DAZGAP010000292         4369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGAP010000292 DAZGAP010000000
VERSION     DAZGAP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.294"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..791
                     /gene="yiaT"
                     /locus_tag="ACHLAM_004990"
     CDS             51..791
                     /gene="yiaT"
                     /locus_tag="ACHLAM_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO7856373.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(912..2891)
                     /locus_tag="ACHLAM_004991"
     CDS             complement(912..2891)
                     /locus_tag="ACHLAM_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO7856374.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHPEVG"
     gene            complement(2902..4302)
                     /locus_tag="ACHLAM_004992"
     CDS             complement(2902..4302)
                     /locus_tag="ACHLAM_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7856375.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
BASE COUNT         1033 a         1150 c         1171 g         1015 t
ORIGIN      
        1 cattactatg ccgactcttt tttatcttat tttaaaatgg aataccggac atgttaatta
       61 accgcaatat tgtggcgtta tttgcgttgc cttttatggc aagcgcaact gcttctgaat
      121 tatccattgg tgctggtgcg gcttataatg aatcgcctta tcgcggttat aatgaaaata
      181 caaaggcaat tccgctgatt agttatgaag gtgatacttt ttatgttcgt cagaccacgt
      241 taggttttat tctgtcgcaa agtgaaaaaa atgaacttag cctgaccgca tcctggatgc
      301 cgctggaatt tgaccctacc gataatgacg attatgccat gcaacagctt gataagcgtg
      361 atagtacggc tatggcgggg gttgcctggt atcaccacga gcgttgggga accgtgaaag
      421 cctctgcggc tgcggacgtt ctggataaca gcaacggctg ggtgggggag ctatcggtat
      481 tccacaaaat gcagataggt cgcctgtcgc tgacacctgc gctgggcgtt ctctattatg
      541 acgagaattt cagtgactat tactatggca tttcagagag tgagtcccgt cgtagcggtc
      601 tggcaagtta ttccgcgcag gatgcctggg tgccttatgt cagcctgacg gcaaaatacc
      661 cgataggaga gcacgtcgta ttgatggcga gcgccggata cagcgagctg ccggaagaga
      721 ttaccgacag cccgatgatt gatcgtaatg agagttttac ctttgtcacc ggcgtgagct
      781 ggcgttttta atttaccggt ggatgtcggt gcggctggcc tacgctctcg caccgactcg
      841 tgggcctgat aagacacgtt aagcgtcgca tcaggcaatg tgctccctac gttggatgcg
      901 gcgcgagcgc cttatccaac ctccggatgg caatgctttt cctcattcac ccagatccgc
      961 atttcgcctt caccccggtt ggcccagcta aaccacggga taaaggtcag ggtttgcggc
     1021 tggcgtttgg ctggcgcgct gtcgtaatgc cacagcggtt gctgctctgg attgctctgt
     1081 tcataccgat aacccggtgc ctggattaag atcttatggc ggaacaatcc cttaccttca
     1141 aatgtcgtaa atggcgcatc ggcgggcagc cacagattat gcagtgactc gccgttgtcg
     1201 gcctgttcca ggcaatacac cagcgggccg cgctgaatcg ccacttttcc ggcgacgtga
     1261 cgcaccagag ggttaccgta aacgcgacgt accggcattg gcaaagtcag attcagcgtg
     1321 tcgccctcct gccattcgcg ggtaatgtgc aaataccctt tacgaatatc ctgctcgacc
     1381 tcttccccat tcaatatgat ctgcggctgt gtgcaccagt ccggcagacg taaagccagc
     1441 gtatgacgca ccggctgggg cgattcaacc gcaattgtca cctgttcctg ccacggatag
     1501 tttccgctaa cccgcagacg cagcgtgcca ttttctaccg gcacttccat gctgtttcct
     1561 gcgtagatgt tgatatacaa cgcatcttca cgcggcgtgt agagataatg accaatcgag
     1621 gttagcacgc gggcgatatt tggcggacaa caagcgcaac caaaccaacg ctggcggatc
     1681 ggcttaacgt gatcgtagat atggttgaat tttaacgttt ttggatgcac ttccagcgga
     1741 ttaacgtaga agaaatgttt gccatccagc gccatgccgc cgagcacagt gttgtacagt
     1801 gcgcgctcca tcacatcggc atattgactg tcgccttcca tttccagcat tcgccgggcg
     1861 aacatcatca ggccgatgga agcacaactt tcggcgtaaa ccgtgtcatt cggcagatcg
     1921 taatcgctgc tgaacgcttc gccgctgctt tgtgagccaa tgccgccggt aatatataac
     1981 tgacgctggg ccatattgtt ccacagccgc aggcaatcct gacgcttgct ttcatcgtga
     2041 cttaaacgcg cgagatgtgc gacgccagtc atcagatata caaagcgtac tgtatgtccg
     2101 atggcggttt gttgctgtgc gatgggcaaa tgcgcctggc tgtaggcttt atctttcacc
     2161 atccacgccg ggccgtaggt gtgccagtgc gaggtctgcc cgcgcttttc atattcctgg
     2221 tcgtaatagt gcggttgcgc accacgctgt tcgacaaaat agttcgtcag cgccaggtag
     2281 cgcggctctt cggtcacttc atacaggcgc attagtgcca gttcaatttc cgggtgacca
     2341 ggataaccgt gtaacttact ttcacctgga ccaaatacgc tgtcgatatg atcggccaga
     2401 cggcaaacca cttccagcaa gcgccgtttt cccgtggcct ggaagaaggc gactccggct
     2461 tcaatcagat gaccggcgca gtaaagttca tgacactccg ccagattgct ccagcgttct
     2521 tcgggcgctt ttaccgtaaa gtaagcattg agatagccat cttcgcactg ggcggaggcg
     2581 atcagttcaa tcacctcgtc agcggttttt tccagttcgg catccggctt ctggcacagc
     2641 gaccaggcta ccgcttccag ccatttggcg acgtcgctgt cctgaaacac catcccgtaa
     2701 aattcaccgt cttgcagtcc tgcggcaatg cgaaagtttt caatcgcatg gctgggttcc
     2761 gcttctggga tacgatcgtt caaggcgtcc cactgataag gaatcaccac atcgcggacc
     2821 agttgttggt actgaccgag gaacggatcg ctgaccgtca gtttatgcag atcgacttcc
     2881 gaaatgttca tcttgtgctc cttaaggctg tacatgacgc tgacgcaggt cgctagcaat
     2941 gcgtgacatc atagggttat tgagtttgca aaagcggatc gcccctgcca gcagcaggtg
     3001 gaatagcgca ggcagtaatg tttccatcgc cgtgatcccg ccaagcgaac tggtggtctg
     3061 gttttccacg cctggctggt aggcgacaaa aataaacagc aggctgatga tcccggcgct
     3121 ggaggcccat gccagcttga tgaaaaacag attgaaggcg aagttcatgc cggaagaacg
     3181 tacgcgggtt ttccactcgc cgtagtcatc ggcaaaggcc atcaatgaga agtgcagtgg
     3241 caatgtgaag ccaagaatca cgccattacc gaggatcacc gccagccaca gcgtttgata
     3301 agccgggcca gaaggcagga accacatcaa caccgccagc gcagcgagga gcaggttggt
     3361 gtagtagtaa attttgacgg tatcgaaacg ccgcgttaac gggctgacaa tcaccgagcc
     3421 gataatggag gcgaaggtga ccatggtgaa gaacagcgac gtgtagcccg tactgccttg
     3481 taagacgtag gtaatgaagt acatataccc accgccgcga atattaaaga cgttaatcag
     3541 caggaaagac atgaccagca tcagcagcag ttggtcgttg ttccgcagcc cggcaagatg
     3601 ttcgcgcagg gtaaatttcc ccattgtgga gagtggcacc cgttcacgaa cccagaagaa
     3661 acagcacagg aacatcacca cggcaatggc gcacagcacg ccgacgccca gttgatagcc
     3721 gcgcgcggca ttaccctgac cgaagagatc caccatccac ggcaggccaa ccgaaaccag
     3781 aaatcccgct acgccgcaga gaacgaatcg ccaggactgg caggagatca cttcattgtg
     3841 gcgggtggtc atggtgttga tcagcgcgca gtacggtacg ttgatggcgg tataaccgac
     3901 tgaaaggagc aggtaagtac cgaacgccca ggcgattttt actcccatgc tggcatctgg
     3961 cacggtgaac gtcaggatgc cgataatgcc gatgggaatg gcaatccata actgccacgg
     4021 acgaaagcgc ccccagcgac tttgcgtccg gtcggcaatg acgcccatca ccgggtcgga
     4081 gatggcatca aacacgcgca gtgcaataaa cagcgtgccg accagcgccg gagttaagcc
     4141 aaaaacgtcg gtgtagaaaa aggtgagaaa gttcatgatc aggcaagtta ttacggtgcc
     4201 gcctgcgtca cccaggccgt agcctatttt ttcccgtaca gataacgcat cgtcaatttt
     4261 acctttagcg atatcagcgg cagtaatcgg tgtagaggtc atagatattc ctcgtgaata
     4321 gggagcaatt tttagtttta ttgcaatgac ctgattaatt ctgccgtga
//
LOCUS       DAZGAP010000293         4364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGAP010000293 DAZGAP010000000
VERSION     DAZGAP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.295"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..339
                     /locus_tag="ACHLAM_004993"
                     /pseudo
     CDS             63..339
                     /locus_tag="ACHLAM_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(448..3963)
                     /locus_tag="ACHLAM_004994"
     CDS             complement(448..3963)
                     /locus_tag="ACHLAM_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO7856376.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTIHTSFSIDFDDSVDKTAETD
                     EEREARYAALQQEWRQYLDDSERLLKNVAGDWIKNPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCKKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGTGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPEKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     VRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRSKRQFQIQLFLEDKLGALIAGNQWSDPETIERNIDELLNSAECEQTTYRKQIDSA
                     HEIFLKEQQAVQEWQRLALDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQEEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGRENSNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVIGIVTPFSAQVSTIKQALGKQGISTGANEKSL
                     TVGTVHSLQGAERAIVVFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPPSSPRGLLAKYLFESEKNALSFDYKERKDLKTSGTKIYTLHGVEQHDNFLNQTF
                     ENTSKYITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHKDYEKRKE
                     KQQNLKGTLEKLNAFGITTKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLEKRQV"
BASE COUNT         1131 a         1085 c          914 g         1234 t
ORIGIN      
        1 caggtcagca gtaactggct atcgtgtata attatccaga agtcctgctg agccttccgt
       61 ctgttcatca tgtgccaggt ttgcgttcgg gatatgatgt gccatcttat gatgatggca
      121 agagatatta ctgcgtacca cctcaattct gtacacatca gtgcccgtcg tttccccggg
      181 atttttcagt gggccaccgg tattcgtacc gaaaatatac cgccattcaa tccgctgaca
      241 gacgaagata atgaacccgt tatggttttt aacggagaga aaaatgtgcc ggcagacagt
      301 ctgaaaccgc agtctttgat tcttccggac agaagataat catgttggta tgaatgttat
      361 aattaaggac tgtgcaataa tagctattcc attcagccat cttacgctcg aaaacgatcg
      421 ataagatggc tactcaccat tcctgctcta aacctgacgc ttctcaagac tattataaat
      481 agcctcaatc tcacccttca ggttatcacc gcaataaacc attgaggtat cgtatcgttc
      541 atatcgcgct tctcgcgttg cgctaaacca gttgaacgat cccacgcaca gcaaaccatc
      601 atcaccaata acaattttgc tatgaacgcg atttaccagc tttgtagtaa taccaaaagc
      661 attcagtttt tccagcgtcc ctttaagatt ctgctgcttc tcttttcgtt tctcataatc
      721 tttatgttca gtgttgtagc ttctgtcagt gactatcgtg acgttaatac cacgtgaaca
      781 cgccgcaatc atggaatcaa gaaaaccggt ttgctccagc ttttgccagg tcagccatgg
      841 agaaactatc gtgatgtatt tactggtatt ttcaaatgtc tgattcagga aattatcatg
      901 ttgctccaca ccatgaagtg tgtagatttt ggtcccggag gtttttaaat ccttacgctc
      961 tttataatca aaagagagcg cattcttctc tgactcaaag aggtattttg ccagcaatcc
     1021 tcgcggagat gagggcggct ggacctcaaa cagatccata tcgccgaaga ccaggaagct
     1081 atccttcgca cgggagactg caacattcag catgctgtta tcgctatcaa taaacccgcc
     1141 gtcttcatgt tttgaataga ctggcgagaa taccacaatc gctctttccg ctccctgaag
     1201 agagtgcacg gtgcccactg tgagcgactt ttcattcgcg ccagtactga taccttgttt
     1261 acccagcgcc tgtttgatag tggatacctg cgcgctaaaa ggcgtcacga taccgataac
     1321 ttcatgaagc gatttgccgt aatgcgcttc aatattttgc tggttctctg ccaaccaggc
     1381 cgctattgtt tcagcctcaa gcaaattata tcgacttcca ctacttgcca gctctccttt
     1441 accatcaata tggagatacc ccattgcggg cattaaattg ctattttcac gccctctttt
     1501 aggcaacaac ttaccgtgat agcagagcgt attacagtat ccaataatat tgtcgtagca
     1561 ccggcggtgt tcatataggt acataccacg agccagttcg ggatcatatt gatagcgcga
     1621 agcaaactgc gctattttca taacgctgcc agatgcggca cttttaccaa ggtctgcgat
     1681 tgccgtatat ttctcggtaa tctcttcttg cgtactgcca gacagaattt tttccgcaag
     1741 catgttaccg acatcaatcg cgggagcaat actccatatt ggcgggatct gctccgtatc
     1801 gccaatcact aatgccttct tagctaatgc aaacgaggca gcagccactt caggaagcac
     1861 ctgcccggct tcatcgacaa tgagtaaatc ggcaaaatca tacaaataac ttttctcgaa
     1921 tttacgttgt cctttgtgct cacttatctg catattgccg ggcagcatat agcatgtcat
     1981 caccacacat ggagtgagtt tcattcgacg ttgccaacgg gcggttaccc ctttagcgcc
     2041 ttttttcttc ttctcttcct gcagatcatc aatactggcc atatccatca accaacgacc
     2101 ttcccagtag tgagtcgtca gtaaaaatgc agggaagcga atctgtgtat cagccagttc
     2161 atcggcctgt gagaagctca gttcctcgtc gccctcatac cctaaatcaa gtgccaacct
     2221 ctgccactcc tgaaccgcct gctgttcttt aagaaagatt tcatgggcgg agtcaatctg
     2281 cttccggtat gttgtttgct cgcactcagc ggaattgagt agctcatcaa tattacgttc
     2341 gatagtttca ggatcagacc actgatttcc tgcaatcagc gcgcctaatt tatcttcgag
     2401 aaacagctgt atttggaact gtcgcttact acgaaccgcc gggagccagg aaaataatga
     2461 atagatcagt gattcaccgg cgcgatattt tttccattcc gttttagcac tcttcagtag
     2521 agtgactttt tgttcttgtc cggaaagtaa tttatttaaa ttatcgagat attgctcaat
     2581 gtcgttagca ataagctcac gcgcagcccg tacctggctt aacgtttgcc atgttgcgtt
     2641 cagtcttatc agttgctcgg attttgctgc caactgacca tgcaggagtt caatgacctt
     2701 ttcaggggat gaacactctt tttcaggaaa agctgcctta gctttctcga gataaaacag
     2761 cagagcatcc tctacatact cttttgactc aacctggttg aagaaatctt cagtttgata
     2821 ttttttggct gcctcagctt tacgactgct tgagggaaaa taagcgccga agcttttcag
     2881 ctctggcaac catcgcccgg ccattgcacc agtgccctgt gaaaaatctt tcccgaacgc
     2941 ttcgataatg ttcgttacag cctggttatt cgttgaagtc gcgataataa ccggaggctc
     3001 agatttttcg agagccgctc tggcccactg cgtggcgatg atagaaagca ccagcgtggt
     3061 ttttccggtt cccggagggc cattaacagc aaggatatcg ccatgtcttg catccagaaa
     3121 atggcttaag gcatcgcgct gagcctttgc cagcggaaac ttatctccgg agtgtccaag
     3181 cctgtcgctg aattttgctc ctggggccag caaagactct gcagcatgaa cctctcgcga
     3241 ggcgaagcga ttgaagagcg gcacatcctt cttgcaaaca agcaggtgat cataaagcga
     3301 aaggatatgg aaactggcac cgccagattg cgccgtttta acaatataac cgtgctcagc
     3361 gagttcatat tgctcaggat ttttaatcca gtcgccggca acgttcttca gcagcctttc
     3421 tgaatcatcc agatattgac gccactcctg ctgcaaggct gcatatcgtg cttcccgttc
     3481 ttcatccgtt tcggcagtct tatcaacact gtcatcaaag tcgatagaga acgaggtatg
     3541 gattgtcttg tatttgtcat actgcccaat ctcaccaatc gaaaatgctc cttttggcaa
     3601 aggttcaagc aggtctctgg gaatggaggt cgctggcgtc ggatataaaa aaccctcacg
     3661 gcttagcaag gctggcgtca ctatcggggt aacaatatca ggcgcacctg cagaacggtc
     3721 cttaccatgc tgcagtaacc ggaaataaac ttttgggcgc aagatgacat cgaccgtttc
     3781 gacatcgtct ttttctccct taaaaaattt actgacaatc gcttcgtcaa gacgtcccgc
     3841 cgctatccca tgccagtgag tgaaattttt ggcgtctttc cgttcaaaac tgccctttcc
     3901 tgactcagca tccgcaagcg agttgcgcca gtatgaggta aaccctaaag cattttcatc
     3961 cattaccagt cctgtgactc aatgaaatcc attgatttat cgaatataaa accaccatat
     4021 ggacaactat tgtccatatt gaaaaagttg gatgcaatgt tttgtttata attcggtatc
     4081 aaatgtactg gttgtacaag gttcagaaca caagcacatt caacagtacg tcaggactat
     4141 tgatttcata acagtgaata cattttttac cgtttataat gactgcctgt gttggacgag
     4201 gataccagaa cagccgaaaa caccgggggt tattacctgc tctcatggaa accagatatt
     4261 cctggtgcta atcacagtca taaatagcct gaagaacgtg acgaagggac ttaaatggaa
     4321 gccggagtaa cttgtcagaa catcaatctt cgtcatcatt ttca
//
LOCUS       DAZGAP010000294         4336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGAP010000294 DAZGAP010000000
VERSION     DAZGAP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.296"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..937
                     /gene="yfeR"
                     /locus_tag="ACHLAM_004995"
     CDS             11..937
                     /gene="yfeR"
                     /locus_tag="ACHLAM_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7856377.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWEVVRGQGRALMAGREGDPLYQI"
     gene            complement(975..1739)
                     /gene="yfeN"
                     /locus_tag="ACHLAM_004996"
     CDS             complement(975..1739)
                     /gene="yfeN"
                     /locus_tag="ACHLAM_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO7856378.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            1987..2820
                     /gene="xapA"
                     /locus_tag="ACHLAM_004997"
     CDS             1987..2820
                     /gene="xapA"
                     /locus_tag="ACHLAM_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO7856379.1"
                     /translation="MSQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2881..4137
                     /gene="xapB"
                     /locus_tag="ACHLAM_004998"
     CDS             2881..4137
                     /gene="xapB"
                     /locus_tag="ACHLAM_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO7856380.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            4190..>4336
                     /locus_tag="ACHLAM_004999"
     CDS             4190..>4336
                     /locus_tag="ACHLAM_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO7856381.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKY"
BASE COUNT         1015 a          951 c         1145 g         1225 t
ORIGIN      
        1 aagaaaaaag atgaattatt ctttaaaaca attaaaggtt ttcgtcacag tagcgcagga
       61 gaaaagtttt agtcgtgcag gagagcgtat cggcctgagc cagtcggcag tgagtcacag
      121 tgtgaaggag ctggaaaatc atactggtgt tcgcctgctg gacagaacca cgcgtgaagt
      181 ggtgcttaca gatgcagggc agcagttggc tttgcgtctt gagcgactgc tggatgaact
      241 taacagcacg ttgcgcgata ccgggcgtat ggggcaacaa ctgagcggaa aagttcgggt
      301 cgctgccagc cagaccattt ccgcgcatct tattccgcaa tgcattgctg aaagccatcg
      361 ccgctatcca gatattcagt ttatcctgca cgatcgtccg cagcagtggg tgatggaaag
      421 tattcgccag ggagatgtcg attttggcat cgtcatcgat ccaggcccag tgagcgatct
      481 gcaatgtgaa gcgattcttt ccgagccttt cttcttgctt tgccatcgcg atagcgcttt
      541 ggccgcggaa gattatgtac cctggcaggc attacaggga gcgaaattgg tgttgcagga
      601 ttacgcgtca ggcagccgac cgctgattga tgcagcactg gcgcgcaacg gtattcaggc
      661 gaatattgtg caggagattg gtcatccggc gacgctgttc ccgatggtag ccgcaggcat
      721 cggcattagt attcttcccg cactggcgct gccgttacct gaaggtagcc cgctggtggt
      781 aaagcgcatt acgccggtgg tggaacgcca gttgatgctg gtgcgcagga agaaccgttc
      841 actttccacc gccgcacaag cgttatggga agtggtacga ggccagggcc gtgcgctaat
      901 ggctgggcgg gaaggggatc cgctgtatca gatatagagc tattcatact gccgggagcc
      961 ccggcagtaa tcaatcagaa attaaatttg atggagtaga ttattgcatc ctggtaaaaa
     1021 tcttcgccca gtttgtcatc cgcataacga tactgaattc ctgtggtgat gtgtgaagtt
     1081 gcattccacc aaagtgcgac ggcaccgttc agaccttctt tgccgccatt acctgccgcg
     1141 taggtggcat cacgatcaaa ctcgtactcg ttccagttgg tgagggtgaa tttctcactg
     1201 cccagcataa agctgtaacc tgcaacccaa ccggcgacat aaccgttatc gccggtataa
     1261 taggtttggt ctgtatagcg ttttgcaaag aacggtttga accaccaacc gctaccggtg
     1321 aaattgtagc cgataccgta aaggaacata tcgtcatgga aattcacgcg attcgcagaa
     1381 ccgtaggtgc catacgcatg caagtagaga ttaaatccgg tgtcacccag gtaaatacgg
     1441 ttggtatttt taaaggtata acgctgctcg ctacccggtt tatcatgacg atcattataa
     1501 aagttttccc agtcgaagaa cccgtacatt tctccccaac taaagttagc gccgccttcc
     1561 agttccagat atccaaaatc atctttgtgt gatttggtcg acgatttctc agtggttctg
     1621 ctagtccagt caagataatg aatacctata tcagcaaaac cgcctttaaa ttctgcatgg
     1681 gatactgctg gtatcgccaa tacagaagag agtgtcagag taaaaagatg ttttttcata
     1741 gcataattcc ctatgccgat cgttattagt tgattgctta ataaaaggtt gattaaaagc
     1801 ggcatattgt taatgcaagg gaaaggagag taccaataca gttttttcag cctattaata
     1861 gtgaaaaaat ataaatatat cgatatcgaa taactatcgg atacatgaaa aaagtgtatt
     1921 ggtatgaggt agggcaatct cgttataaaa aggcgttaga ttccacccta caaaaaaagg
     1981 atatatatgt ctcaggctca gttttctcat aacccactgt attgcgtaga tattatcaag
     2041 acttataaac ctgatttcac gccacgagtg gcctttattt taggttccgg gctgggcgcg
     2101 ctggccgatc agattgagaa cgcggtcgca atttcctacg aaaagctgcc tgggttcccg
     2161 gtaagtaccg tacacggtca tgcgggtgag ctggtgctgg ggtatctcca gggggtgcca
     2221 gtggcgtgca tgaaaggtcg cggacatttc tacgaaggtc gtgggatgac catcatgacg
     2281 gatgcaatcc gtacctttaa gttgctgggc tgcgagttgc tgttctgcac caatgcggct
     2341 ggctcactgc gtcctgaagt gggggccggc agtctggtcg cattgaaaga tcacatcaac
     2401 accatgccgg gaacgccgat ggtgggtctt aatgatgaac gttttggtga gcgcttcttc
     2461 tcgctggcga atgcctacga tgcggaatac cgcgcactgt tacaaaaagt ggcgaaagaa
     2521 gaggggttcc ctctgacgga gggcgtgttc gtctcgtatc cggggccgaa tttcgagact
     2581 gcggcggaaa ttcgtatgat gcaaattatt ggtggggatg ttgttggtat gtctgtggtg
     2641 cctgaggtta tttcagctcg ccattgcgaa cttaaagtcg ttgcggtctc tgcgattacc
     2701 aacatggcgg aaggtctgag tgacgtgaag ctttctcatg cccaaacgct ggcagcagcg
     2761 gaactctcaa agcaaaactt tattaatctt atttgcggct ttctgcgcaa aattgcctga
     2821 gtaaaacaac aataaacggc cttactgagt gaggccatat gatgcgacaa ggaaacgatt
     2881 atgagcatcg ctttgcgctt aaaggtaatg tcctttttgc aatattttat ctgggggagc
     2941 tggctggtta ccctcggttc ttacatgatt aatacccttc atttcaccgg cgctaatgtt
     3001 ggcatggttt acagttccaa agggatcgcc gcgattatta tgccgggtat aatggggatc
     3061 atcgcagaca aatggctgcg tgcagaacgt gcatacatgc tgtgtcacct ggtgtgtgcg
     3121 ggcgtacttt tttatgcggc atccgtaact gatccggata tgatgttttg ggtgatgtta
     3181 gtcaatgcga tggcgtttat gccgactatt gcgttatcga acagcgtctc ttattcctgt
     3241 cttgcccagg cagggcttga cccggtgacc gctttcccgc ccattcgcgt ttttggtacg
     3301 gtggggttca ttgtcgccat gtgggcagta agcctgctgc atctggaatt gagtagtctg
     3361 cagctgtata tcgcgtccgg tgcgtcattg ctgctgtcgg cttatgcgct gactttgccg
     3421 aagattccgg ttgcggagaa aaaagcgact acatcgcttg ccagcaagct gggtctggat
     3481 gccttcgtgc tgtttaaaaa tccacgcatg gctatctttt tcctcttcgc catgatgctg
     3541 ggcgcggtgc tgcaaattac caacgttttt ggcaacccgt tcctgcatga tttcgcccgt
     3601 aatcctgagt ttgctgatag cttcgtggtg aaatatccgt ccattttact gtcagtttca
     3661 cagatggctg aagtcggttt cattctgacg ataccgttct ttttgaagcg tttcggtatt
     3721 aaaaccgtca tgctgatgag tatggtggcg tggacgctgc gctttggctt ctttgcctat
     3781 ggcgacccat caccaaccgg gtttattttg ctgctgctgt cgatgattgt ttatggctgt
     3841 gcattcgatt tcttcaatat ttctggttcg gtatttgtcg aacaggaagt tgattccagc
     3901 attcgtgcca gcgcccaggg cctttttatg accatggtaa atggtgtcgg cgcatgggtt
     3961 ggctcgattc tgagcggtat ggcagtggat tacttctcgg tggatggcgt aaaagattgg
     4021 cataccattt ggctgatgtt tgcagggtat gctcttttcc tcgcagtgat atttttcttt
     4081 gggtttaaat ataaccatga ccctgaaaag ataacgcatc gttcagttac acattaaaag
     4141 gattcgcagc tctgttcctt ggggctgctt ttatgataaa ggttaattag tgaaagatat
     4201 tttattttct tctggcgttg gttttggcat tggtgctttg tttacaatcg tgcgcttgcc
     4261 tattcctgta ccaaatgtat taccgggtat attatccatt gtgtttatgt atgtcggata
     4321 tctggtggtg aaatac
//
LOCUS       DAZGAP010000295         4266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGAP010000295 DAZGAP010000000
VERSION     DAZGAP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.297"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..197
                     /gene="cui"
                     /locus_tag="ACHLAM_005000"
     CDS             15..197
                     /gene="cui"
                     /locus_tag="ACHLAM_005000"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="HFO7856382.1"
                     /translation="MYAFLFADTELTSSAKMLKLMSVNIILLSFFYISLHSIIFIFSY
                     LYFWFCIGSYRSFKEK"
     gene            complement(220..1716)
                     /locus_tag="ACHLAM_005001"
     CDS             complement(220..1716)
                     /locus_tag="ACHLAM_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO7856383.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGS
                     GNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDV
                     QGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVG
                     SEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVD
                     VEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNE
                     EPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLAR
                     EIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRDALVNAWRSRDAQDIANK
                     LGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLS
                     AIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINNKLVHFTH"
     gene            1955..2245
                     /locus_tag="ACHLAM_005002"
     CDS             1955..2245
                     /locus_tag="ACHLAM_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856384.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            complement(2237..2515)
                     /locus_tag="ACHLAM_005003"
     CDS             complement(2237..2515)
                     /locus_tag="ACHLAM_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856385.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRASLAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            2636..3058
                     /locus_tag="ACHLAM_005004"
     CDS             2636..3058
                     /locus_tag="ACHLAM_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO7856386.1"
                     /translation="MRSAVARLLLIPLITATIVLTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGVKDKAAGVSAETAQQRIKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            3456..>4266
                     /locus_tag="ACHLAM_005005"
     CDS             3456..>4266
                     /locus_tag="ACHLAM_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014228715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7856387.1"
                     /translation="MTKLYIYLVLAASLSALSTDMIVPALPELQKAFHADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANDLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKETNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFHYSVVFSLSVLFFIIGNQLSKIEKISNPWVLFPLNAISV"
BASE COUNT         1286 a          986 c          721 g         1273 t
ORIGIN      
        1 tttatgctat cttaatatat gcttttttat ttgctgatac ggagctaacc agttctgcaa
       61 aaatgctaaa actgatgtct gtaaatataa tcctcctatc gtttttttac attagcttac
      121 attccattat ttttatattc tcatatttgt atttttggtt ttgtattggt tcctataggt
      181 cgtttaaaga gaagtgatat gatcacctct ctttttctat caatgagtaa aatgaaccaa
      241 tttattattt attttttcag ccacacttgc atcaatatat gatgcaaggt aactaattcc
      301 cataatacct gcaatattta ttgcagttaa tgctaacgcc ggtagtgtga atgatccaat
      361 tatagcacta agcaatgtca tggcaacgct tgcggccata ccactgagaa cccaagactc
      421 aacctccaac atcagcggcc cccagtttcc agtttcatat ccctcgatgc ttttctcctt
      481 gaccttctca atcttcataa ttacatcggc agctttgaaa gccttactga tattccccag
      541 tttatttgct atatcctgag catcacgact tcgccaggcg ttcaccagtg catcacggtc
      601 tgctctgtta actttaaaac taggattaga caaaatttta tttaatgact ccattgcatc
      661 atcgtagcta cggatggttt tcccctggaa atttttgata tcatcagcaa tttctcttgc
      721 taacgactta taagattcac caagataatc accgatcttg ttacccatat cagatataag
      781 ttcagctgct ttatttagca catcactctg aatccggtca cggttcatac tgaaagaata
      841 aaatacatca taagcagctt ttttagcttg attctgtcgt tgaggctctt cattattaaa
      901 tcctaatgcc tcgtatatct cttccggggt cttatggtcg actttatcca taataccagc
      961 cttagctttt tcactcccta cataaggacg aacagctgtt ctagttacag ataaaacatt
     1021 accataatca tcaagcacca ctaaatagtg gaactgttca acatcaacat caaattttct
     1081 cacattagga ggcaaacgat cccacgcatc ttcagcctgt tgttttgctt tttcctgccg
     1141 ctgagattca tctttattaa aacctaacgc ctcatatatc tcctcaggtg ttttatgatt
     1201 gattttttcc ataatacccg ccttagcttt ttcactccct acgtaaggac ggacacctgt
     1261 tcgaagaata ccgatagctc taccattatc attaagcgtt acacgatatt taaaaccatc
     1321 gatactcaca tcaatctctc gcggtaagtt gtttttatcc cacttacgag caacttcatc
     1381 attaggattt tcaggaatat agcccccctg aacatcacta tttccgccag cctcaggaac
     1441 tccatataca ggcgttttta ccagaccatt tgtaatggta atgcgcacct gcccattatc
     1501 atcaacataa ggcggcacat ccggattatt ggttttactc catccccacc ccgtctgact
     1561 tccatgttca ccacctccat taccagagcc tgaaccacca ccccagtgaa ccccactatt
     1621 actattatta tttccacctt gctgattcag attcgcccct gttcccccca tagactcacc
     1681 agcagttggg ccatatccac ttagttcttt agccataaat tcctctttga taattaaaac
     1741 aataaattaa aaacaacaca ctgtacatat aaccactggt tttatgtaca gaaaaaccta
     1801 ctactcacca ttgtccatgt caagagcatg gattttcatt tttgcaataa ggatcacact
     1861 atggggaggc aggcattgag aacgtcgaaa cagaacaccg gagcaaatca ggatgagata
     1921 taaaactgtt ggatcatgaa aaacggagaa cgatgtgagc aaatcacccc gccataaact
     1981 gaacaaaaca gacaaacgac ttctcgacac ccttgttgct gccggatatg agcatgacaa
     2041 agcccgtgac ctcatccaga aacaggttta cacgctgaca ctggctgatc agcgccatgt
     2101 ggtcagtgaa atcagtaacg gtgtgaatcc cacccaggct tactcggcgg tataccaggc
     2161 aagacgcatt cgcctcgccc gtaaatatct gaacggtaaa aaggttatgg aagaaaccgg
     2221 ggaaaatacg cccccatcag cgtaaggatt tcttttgccg ctccagagac tccagttttt
     2281 tacgcaaatc ctctcttttt tgggcatctc tggtgccagc cagagatgcc ctcaactcat
     2341 cgatctgaag ttgtatcttc agtctgttac tgaacatttt ctgtctggca ttaacatccg
     2401 caacaatgcc attttttgtc ttctcggcct tttgttgaaa aacattgctg tccgcatgac
     2461 tggcaaccga agcagaaaga acactaaaaa gcaggactgg cacacatttt ttcacgggat
     2521 tattcctgac tcattgacca tcaaatcaca ttggggtaaa ccgacgtatg ataagagata
     2581 ctcttcggaa atataactcc ctgagtatca agattaaaaa cgcaaggaga tgtttatgag
     2641 atctgccgtt gccaggctgc ttctgatacc tctgataaca gcaacaatcg ttcttacagg
     2701 atgcacacca aagaccagcc tggaacgaca tacccggcat tatgtttatg cttcagatga
     2761 tggatttgat cctaacttct acacccagaa agcagacacc atacgtatga tgctcccgtt
     2821 ctttcagcag ttccgggata tgggggtgaa agacaaagca gccggagtat cagcagaaac
     2881 ggcacagcaa cgtataaaag aattccactc agaaaaattt tttcactcac tccggagcac
     2941 aacaaccttt gctggcagaa aatacacaaa cagcgatatg ccgtcgccga aaaaaatgaa
     3001 actaatggca gacaccattt ctgcggttta tctcgatgga tacgagggca gacagtaagg
     3061 ggatttacca taatccctta attgtacgta ccgctgaaac gctctcatac gcgttctgac
     3121 gcaaaaacgc aaaaagacaa ggaaacccca ggctaaaccg aaaaaatgcg tcagaatcgt
     3181 tttcaggtac ttgcataact attcacaaaa gcgatgcgcg atcgatttcg actattccga
     3241 cggacgaaca ggttaaaatg ataagtttcc tccctccgga tggaaggttc agcacgggga
     3301 tttttacccc cttccatcaa cctgaatatg catctgggag tacccattag tttctactca
     3361 aacactatcg tagtgctatc tcaaaatata aaacataccg atggcgtaag cattttaaat
     3421 atgataaata agaatatttt tatcagggat taattatgac aaaactatat atttaccttg
     3481 ttcttgctgc atcattatcg gcattatcaa ctgacatgat tgtgcctgcg ttaccagaac
     3541 tacagaaagc atttcatgct gactactcat taattcaact aagtattagt ggatttctta
     3601 ttgtatttgc atttagtcaa ctgatatcag gaaccatagg tgattatttc ggaaaagtac
     3661 gcgtaatgac tatatgctta attatatttc ttattggcag tattgtatgt tttatggcca
     3721 atgatttaaa tatgcttctt ttgggtagaa ttctacaggc aataggtgca ggtgccggac
     3781 ctgtaatatc aaaagccatt gctaaagagt cttttccacc actaaagcta aaaagagcac
     3841 tttctgacat atcatcaaca agtgcgataa taccattaat agcacctctg gccggagcat
     3901 taatacttga ttattataac tggaatgtaa tatttattgt aatgggtgca tttagcattc
     3961 taaccatact cctgtcacct aaagagacta ataaacagga gaaaaaaaca catcaagata
     4021 atgtaaaatt tgtgactaaa gatttcatct gcgggacaat actcgtttca ctaatgctaa
     4081 gctcactatt ttgctacata tcaatatcac ctgcactatt tatgagtgat tatggattaa
     4141 atacatttca ctactctgtt gttttttccc tgtcagtttt gtttttcatc attggaaacc
     4201 aattgtcaaa aatcgaaaaa atatcaaacc catgggtctt atttccccta aatgcaatat
     4261 cagtta
//
LOCUS       DAZGAP010000296         4217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGAP010000296 DAZGAP010000000
VERSION     DAZGAP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.298"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1863)
                     /locus_tag="ACHLAM_005006"
     CDS             complement(<1..1863)
                     /locus_tag="ACHLAM_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFO7856388.1"
                     /translation="MTDITANVVVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVAQPIVINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQLEQRLSSTSGASLIGIDKYGTIENAIKYSIKYVTPEMYISQSSDITE
                     AIQMAIDDCEIVYMEPTDYLIERTINIPDGRVIYLGGSKLTASTGSSPIFKYNGKNIG
                     LTIWGGSCIVTGTASAFLEAEGDSDTPSNEDYIKQIRLYGVHVSSSMITYALLLKKAV
                     RQIFIDSCMFYTVNGIVSSGKTVELACNKSIVFGATSATDTVGIGIYSTATGGPYYNE
                     GWHFTDCTIDNFERTFDVSDIYVLTVNGGFIGNNSNTGFAMYFGEGNTTHCREINVNT
                     VIGGRVKFNNATIERLCHAKFSGEITYCKGGGGVCFAIGNNISGVDVIGMKFTTNTGH
                     VVATVGNGSSNIHFSDISTDSSHTSGILFSGTDGGNCSISDFSYSGAGEALSLPRPVK
                     LSNVPVIGVATPVFSIKNDFVSSNKIVAIGSNINQLQMTIAKGSRISININISYLGGA
                     GSTTQVIGITAPSGTILPNGSNIMQFTLPDVNGSVTLNVVASVSTDFINGAFTID"
     gene            complement(1964..2842)
                     /locus_tag="ACHLAM_005007"
                     /pseudo
     CDS             complement(1964..2842)
                     /locus_tag="ACHLAM_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
     gene            complement(2911..3072)
                     /locus_tag="ACHLAM_005008"
     CDS             complement(2911..3072)
                     /locus_tag="ACHLAM_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO7856389.1"
                     /translation="MKGMSKMPQFNLRWPKEVLDLVRKVAEENGRSVNSEIYQRVMES
                     FKKEGRIGA"
     gene            3163..3417
                     /locus_tag="ACHLAM_005009"
     CDS             3163..3417
                     /locus_tag="ACHLAM_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO7856390.1"
                     /translation="MARDDPHFNFRMPLEVREKLKYRAESNGRSMNSELLQIVQDALS
                     RPSPVKGYRNEAERLAEMQAEQFKSVVFETLKNMYGKDAK"
     gene            3414..3995
                     /locus_tag="ACHLAM_005010"
     CDS             3414..3995
                     /locus_tag="ACHLAM_005010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856391.1"
                     /translation="MIDYNPAKKEIIAAKKCLEQMKSSTNHDDFEMHWRECLGHIEKS
                     FSKLLCATKSVSGKFSSHFNQKFMLRKTDKTLAYLHQARNADHHSTMEISKLEPPYTT
                     LGAFPGARSHYIKELIIDSTGKIAKYEGDPMIVEFHPATTMPITVRNQGKDYPPPTEH
                     LGEKLVDIHPSVLAYLGIQFYEKWIDESRAMFR"
BASE COUNT         1264 a          909 c          769 g         1275 t
ORIGIN      
        1 atctatggta aaagctccat taatgaaatc agtagaaact gaggcaacta catttaaagt
       61 aacacttcca ttaacatcag gaagagtgaa ttgcataata ttgctgccat ttggaagtat
      121 agtaccactt ggcgcagtaa ttcctattac ttgcgtcgtg cttccagcac caccaaggta
      181 agaaatgttt atatttattg aaattctact acctttagcg attgtcatct gcaactgatt
      241 tatatttgaa cctattgcta ctatcttgtt agaagataca aaatcatttt ttattgaaaa
      301 aacaggtgta gcaacaccga tgactggtac gtttgaaagc ttaacaggcc ttggaagtga
      361 taaagcctcc cctgctcctg agtatgaaaa gtctgatatt gaacaattac caccatcggt
      421 gccagaaaac aatattccag aggtgtgtga agagtctgtg cttatatcag aaaaatgaat
      481 atttgaagaa ccattaccaa cagttgctac aacgtgtcct gtattagttg taaacttcat
      541 accaataaca tcaactccag atatattatt gccaatggca aaacacaccc cacctccccc
      601 cttgcagtag gttatctcac cgctaaattt tgcatgacac aatctctcga tagtggcgtt
      661 gttaaactta accctacctc ctatgactgt attaacatta atttctctac aatgagttgt
      721 atttccctct ccaaaataca tagcaaatcc agtatttgag ttatttccta tgaatccacc
      781 attgacagtt aacacatata tatcagagac atcaaatgtt ctttcgaaat tatctattgt
      841 gcaatcggta aaatgccaac cctcattata ataagggcca ccggttgcag tactataaat
      901 gccaattcca acagtatcag tagccgatgt cgccccaaat acaatgctct tattacaagc
      961 caactctact gttttccctg atgaaacaat cccatttacg gtataaaaca tgcaggaatc
     1021 aataaatatt tgacgaactg ctttttttaa caaaagcgca tatgtaatca ttgaagatga
     1081 tacatgaaca ccgtaaagtc ttatctgttt aatatagtct tcattgctag gtgtgtcgct
     1141 atcaccttca gcctctaaga atgcacttgc cgttcctgta acaatgcagc tgccgcccca
     1201 aattgttaat ccgatatttt taccattata cttaaatata ggagaactac ctgtagaagc
     1261 tgttaacttt gatccgccaa gataaataac cctgccatca ggtatattta ttgttctttc
     1321 aataaggtag tcggtaggtt ccatatatac aatttcacaa tcatcaattg ccatctgaat
     1381 agcctcggtt atgtctgagg actgtgaaat atacatttct ggtgttacat actttattga
     1441 atatttaatt gcattctcaa ttgtaccata tttatctata cctattaatg atgctcctga
     1501 tgtgcttgaa agtctttgct ccagctgatc agggtcatac ttcagcacat taggaaaata
     1561 gaactgctgc gcaccatatg catcataaac agccatagaa tggccttgca cggtaacgaa
     1621 tttggcaatc tgtccgttat atactggata cccagcggca ttgatgacga ttggctgtgc
     1681 aacaggaacg tgagaaccat cttcgttctc tacataaacc tgaatttggt tttcaggatt
     1741 taccgggtca gtgtcaattt taccgatata aatttttcca ttggctacgg ctttaaaaga
     1801 acgcgccata gtgaagagtt gcgaaggcat gctcactaca acattggctg taatgtctgt
     1861 catttaattt gctccagata caaggaatcg ccgcagcatg gctacggtga attttgggca
     1921 taaaaaaacc cagccgaagc tgggtcgttg agttggttat ctgtcagtag ttatgtactg
     1981 aaggaggtaa ttctttattc ttaagtctca tccatgcgga aagattcgtt ggtccgtctg
     2041 gctcattaat atcaacatct cgtgtatggt tgattaaaac atctctcgcc attccgataa
     2101 catacgagaa ctcatgaccg tagtcgtagc atctgccgga atagttcgat tgaatttgtt
     2161 ttaatgccgg atacagttca cggaataatg cctgtgaacg gttggcataa tcccatagcc
     2221 atacaaggct gtttgcttct tttgcagaaa gctcgtttgc tttcttctct tgtttgccga
     2281 taaactcgcc ttcaagcact accctgtgga tgtactatac ggccagcggg atttgttcaa
     2341 ttgaaagttc atcaatgctg tcaataccaa aacgctgatg aaccatattg tatgcatcgt
     2401 catagcgaag tcctttcttt cctaccagca tgtttactgc atcgcgtagc ggcgttcttt
     2461 cctcaacagt ggttttcttg cctttcacat actcgccatg tttgcgaatt gaaggtagaa
     2521 cttctgctgt tacccacttg cggaatttgt gcgggaccga acctttattg acagcatcgc
     2581 ggcagcgcag aaccaatgta tacatacctg attcgctaac aatgcttaga ttctgctcac
     2641 caccaagggt gtaacttaaa gttactccct tttcatcgtc atcaagtgca gtaatcgcct
     2701 tgcgtgagtt agtcagggtt aaagcatcac aaacatcttt tgctacaaac cacggctcac
     2761 cgcatttgtt gatgacgcgg atttcactgt cgccgaattt gaagatggtg aaatcgtttt
     2821 gtgcctttgc tatacttttc atgtcaatat ttcctaatcc gatttgttga taccgaagcc
     2881 ctgactgttc ccgcagttgg ggcttcaact ttacgcgcca atgcgccctt ccttcttaaa
     2941 gctttccatt actctctgat aaatctcaga gttaacagac cgaccattct cttccgccac
     3001 cttgcgtacc aaatccaata cttctttagg ccaccgcaaa ttgaactgcg gcatcttgct
     3061 cattcctttc atattcacct cacaatatag gtccaccgtg gacctattga gaatatagta
     3121 gagtgcttct atcatgtcaa tacactaact tggagtgatg gcatggctag agatgatccg
     3181 cactttaact tccgcatgcc tttggaagta agagaaaaat taaaatacag agcagagtca
     3241 aatggtagat caatgaactc agaattgttg caaatcgtcc aagatgctct atcgagacca
     3301 tctcctgtaa aaggttatcg caatgaagct gaacgattag ccgagatgca ggctgaacag
     3361 ttcaagtctg tggtatttga gacacttaaa aacatgtatg gcaaggatgc aaaatgatcg
     3421 attacaaccc agcaaaaaaa gaaatcatcg cagctaaaaa gtgcctagag cagatgaaat
     3481 catctacaaa ccatgatgat tttgaaatgc actggcgaga atgtcttggg catatagaaa
     3541 agtctttcag taagttgctc tgtgcaacaa agtcggttag tggaaaattt agcagccact
     3601 tcaaccaaaa gttcatgctt agaaaaacag acaaaacact tgcttacctt caccaagcaa
     3661 gaaatgctga tcatcattcc actatggaaa tctcaaaact tgaacctcct tatactactc
     3721 ttggggcatt tccgggagca agaagccatt atattaaaga gttaatcatt gattcaacag
     3781 ggaaaatagc aaaatacgaa ggcgacccta tgatagttga gtttcatcca gcaacaacta
     3841 tgcctataac cgtaagaaac caaggcaaag attatccacc tccaacagaa catcttggcg
     3901 aaaagcttgt tgatatacat ccatctgttc ttgcctatct cggtatacaa ttttatgaaa
     3961 aatggataga tgagtcgcgt gcaatgttta gatagttatt tttgataatt tcaagtattt
     4021 cctcttgact ttgatttacc caaacgtgat cacactcatg aaaccagatt atgcttcctg
     4081 tttcgccttg tgcaatatag gtgatcatgt tttcattaac aattattact gagttatcat
     4141 gtgcgcaatt tagttttatc aattcgcata tcctatcgtg acaaagtaac tagaaaaact
     4201 aaagaggttg gtgtgtc
//
LOCUS       DAZGAP010000297         4085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGAP010000297 DAZGAP010000000
VERSION     DAZGAP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.299"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1967)
                     /gene="speA"
                     /locus_tag="ACHLAM_005011"
     CDS             complement(<1..1967)
                     /gene="speA"
                     /locus_tag="ACHLAM_005011"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO7856392.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYL"
     gene            complement(1976..2107)
                     /gene="yqgB"
                     /locus_tag="ACHLAM_005012"
     CDS             complement(1976..2107)
                     /gene="yqgB"
                     /locus_tag="ACHLAM_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO7856393.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2201..2344)
                     /gene="yqgG"
                     /locus_tag="ACHLAM_005013"
     CDS             complement(2201..2344)
                     /gene="yqgG"
                     /locus_tag="ACHLAM_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO7856394.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2455..2706)
                     /gene="yqgD"
                     /locus_tag="ACHLAM_005014"
     CDS             complement(2455..2706)
                     /gene="yqgD"
                     /locus_tag="ACHLAM_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO7856395.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2762..3916
                     /gene="metK"
                     /locus_tag="ACHLAM_005015"
     CDS             2762..3916
                     /gene="metK"
                     /locus_tag="ACHLAM_005015"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO7856396.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1026 a         1121 c         1004 g          934 t
ORIGIN      
        1 tcaagatagg tataaccgta caaacctgcc tcgaactctt caaggaactg ttgttgcagt
       61 tcagcatcaa gatcggtttt cttcacttga tcacggaact gggttaacaa cgttttcgga
      121 tcgagctgta catattgcag catatccgcc acggtatcgc cttcgtcaga cagttctact
      181 tctacgctgc cgtcagggaa gacgaacacg tcaaccgctt cggtatcacc gaacaggttg
      241 tgcatgttgc cgaggatctc ctgatatgcg ccgaccatga agaaaccgag catcggcgga
      301 ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc atcgatgtag
      361 tgatcaatag caccgtcaga atcacaggta atatccagca gcacagcgcg gcgctccggc
      421 acttgatcca acccttccag cggcagaacc gggaacaact ggtcgatccc ccatgcgtcc
      481 ggcatcgact ggaacagcga gaagttgacg tacattttgt cagccatacg ttcctgcagc
      541 tcgtcgataa tcggacgatg cgcacggttt tgcggatcca gttgcttctg cacttcatgg
      601 cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa ggtgccggaa
      661 gagtagccga tatgaatatc gtgcagatcc atctggctgt cgtgtaacca ttcacgcaga
      721 gaacgacgag ttcccggttc gtgcatctcc tgccaggttt cccacatgct ttgcagggcg
      781 cgcggtgcgt cttctgcagg cgcggtcggc accgtgtatt cgttacgttc cacgccgatg
      841 atattcgaca ccagcacggt gtgatgcgca gtcaccgcac gacccgactc ggtgattacc
      901 gtcggatgtg gcagaccatt ttcttcacac gcatcaccaa tcgcccagat gatgttgttg
      961 gcgtattcat tgaggccgta gttcaccgaa cagtcggact gcgaacgggt gccttcatag
     1021 tccacgccca gaccgccgcc aacgtcgaag cactgaatat tgacgcccag cttgtgcagt
     1081 tccacataga aacgcgcgga ttcacgaacg cctgtcgcga tatcgcgaat attcgccatc
     1141 tgcgaaccga ggtggaagtg cagcagttgc aggctgtcga gacgcccggc ttcacgcagc
     1201 gtttcaacca gttgcagtac ctgagtcgcc gccaggccga acttcgattt ttccccgcca
     1261 gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc cagacgagga
     1321 acgacattca gacgttctgc ttcatccagc acaatggcaa tttctgacat cttctcaatg
     1381 accagataga ccttgtggcc catcttctcg ccaattaatg ccaggcggat atattcgcgg
     1441 tccttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc cagcaccgcc
     1501 atcaactccg ctttggaacc ggcttccagg cccagcggtt cgcccgaatg aatcagggac
     1561 tcaatcacgc ggcggtgctg gttaactttg atcggataaa cgaggaagta atcgccgtta
     1621 tagccgtagg attcccgcgc acgtttgaac gcggcgttaa tagaacgcaa acggtgctgc
     1681 aggatctgtg ggaaacagaa cagtgcaggc agacgctggc cctgtgcttc acgagttttc
     1741 actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca cacgctaatg
     1801 tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt gtaagtacgc
     1861 agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg tagtacaccg
     1921 tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc gaacctcaaa
     1981 ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc gataacaacc
     2041 cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca accggtttct
     2101 ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg cgaaaccacg
     2161 agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg taaccagctc
     2221 ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa gcagagatgc
     2281 agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc agacaacggt
     2341 tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag ccaaaactct
     2401 gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt ttatttaagc
     2461 cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa tacgcagaat
     2521 gccagcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac acgattcaaa
     2581 aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt taatccatcc
     2641 ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg taggatccgc
     2701 taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg tgatattaaa
     2761 tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg acaaaattgc
     2821 tgaccaaatt tctgatgccg ttttagacgc gatcctcgaa caggatccga aagcacgcgt
     2881 tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa tcaccaccag
     2941 cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg gctatgtgca
     3001 ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg gcaaacagtc
     3061 tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg cgggtgacca
     3121 gggtctgatg tttggctacg caaccaatga aaccgacgtg ctgatgccag cgcctatcac
     3181 ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca ctctgccgtg
     3241 gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca aaatcgttgg
     3301 tatcgatgct gtcgtgcttt ccactcagca ctctgaagaa atcgaccaga aatcgctgca
     3361 agaagcggta atggaagaga tcatcaagcc gattctgccg gctgaatggc tgacttctgc
     3421 caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga tgggtgactg
     3481 cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac gtcacggtgg
     3541 cggtgcattc tccggtaaag atccatcaaa agtggaccgt tccgcagcgt acgcagcacg
     3601 ttatgtcgcg aaaaacatcg tggctgctgg cctggccgat cgttgtgaaa ttcaggtttc
     3661 ctacgcaatc ggcgtggctg aaccgacttc catcatggta gaaactttcg gtactgagaa
     3721 agtgccttct gaacaactga ctctgctggt acgtgaattc ttcgacctgc gcccatacgg
     3781 tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag catacggtca
     3841 ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc tgcgtgatgc
     3901 tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc gctggccttt
     3961 ttcttttacc tcaccgcggt ggacaggcat tcacgccgca tccggcaaaa aaaccgcccg
     4021 cacaataaca tcattcctcc tcaacacgtt ttaccgcaaa ttaccatcac tactgaaacc
     4081 gatta
//
LOCUS       DAZGAP010000298         4081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGAP010000298 DAZGAP010000000
VERSION     DAZGAP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.300"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1343
                     /gene="hprS"
                     /locus_tag="ACHLAM_005016"
     CDS             <1..1343
                     /gene="hprS"
                     /locus_tag="ACHLAM_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO7856397.1"
                     /translation="TVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTTLINRTA
                     QIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGHGINKIVNRTNVSDGMLNNIPASE
                     TISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKINSIIICI
                     VAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPLGQALNK
                     MHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIAGNIEEL
                     ENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICLKVECNQ
                     QIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTKIHEPEK
                     LFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQRN"
     gene            complement(1450..2301)
                     /gene="hchA"
                     /locus_tag="ACHLAM_005017"
     CDS             complement(1450..2301)
                     /gene="hchA"
                     /locus_tag="ACHLAM_005017"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO7856398.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2893..3966)
                     /locus_tag="ACHLAM_005018"
     CDS             complement(2893..3966)
                     /locus_tag="ACHLAM_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7856399.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRDVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         1145 a          830 c          924 g         1182 t
ORIGIN      
        1 taaccgtccg tttaaccttg ctttttatat tggtgctgtc tgttgctggc gctggaattg
       61 tatggactct ctataatggc ctggcaagtg agttgaaatg gcgcgatgat acaacactca
      121 ttaaccggac agcgcagatc aagcagttgt taattgatgg ggtaaatcca gatacgttac
      181 ctgtctattt taaccggatg atggatgtta gtcaggatat cttgatcatt catggtcatg
      241 gcatcaataa aattgttaac cggacaaatg tcagtgatgg catgttaaat aacatacctg
      301 ctagtgagac aatcagcgca gctggcattt acagaagcat tattaatgac acagagatag
      361 atgctttacg aattaatatt gatgaagttt cgccatcatt aacggttact gtggctaaat
      421 tggcttcagc cagacataac atgcttgaac agtataaaat taatagcatt ataatttgca
      481 ttgtcgccat tgtactttgc tcagtattaa gtccgctgtt aatcagaacg gggttacgag
      541 agatcaaaaa gttgagtggt gtaacggaag cgctgaatta taacgataac cgggagcctg
      601 ttgaggttag cgcattaccg agagaactaa aacctcttgg gcaggcgttg aataaaatgc
      661 atcatgcttt agtcaaagat tttgagcgtc taagtcagtt tgctgacgat ctcgctcatg
      721 aacttagaac accaattaat gcattactgg gtcagaatca ggttacgctc agtcaaacca
      781 gaagtatcgc tgaatatcaa aaaacaattg ccggaaacat tgaagagctg gagaatattt
      841 cgcggttaac agagaacata ctgtttcttg ccagggcaga taaaaacaat gttttggtga
      901 aactggattc gctttctctc aataaggaag tcgaaaattt gttggactat cttgaatacc
      961 tttcagacga gaaagagatt tgccttaagg tcgagtgcaa tcagcaaatc tttgcggata
     1021 aaattttact gcaacgaatg ttatcgaatc ttattgttaa cgctattcga tattcaccag
     1081 aaaaatcgcg cattcagata accagttttc ttgataccaa cggctctctt aatattgata
     1141 tcgccagtcc tggaacgaaa attcatgagc ctgaaaaact cttccgtaga ttttggcggg
     1201 gagataattc gcgtcattcc gtaggtcagg gacttggtct ttctttagtc aaagcgattg
     1261 ccgaattgca tgggggaagt gctacgtatc actatctcaa taagcataac gtgttccgaa
     1321 ttacgttacc gcaaagaaat taatataggg cttcagtacg ccaaaattaa ccgtgaaaaa
     1381 acgcagtcac gtcgtgagta ttaacgtggc tgcgattgat aatgcgctta cattacaatg
     1441 taacagggat taacccgcgt aagctgccag catttcctgc gccgccagtt tacccaacgc
     1501 attcgctgcg aaaggactgt cgccggtgag aactttacgg tccttatgta ctcgcccggt
     1561 gatgtcgtca ttaatgatgt tcatgcccat tttcttcagc tcttcgccga agtaccaggt
     1621 gagatgaccc ggcatatagc ctatctctgg cgtttgtttg tctgcggcgt ctgggaatgc
     1681 gcaaatggaa taaccattca gtgggttatc gccgtggcga agcgccagaa aagccgccgg
     1741 gccgtggcaa agggagataa caaaacggtc atttttgatt gcccactgta aagcggcagc
     1801 tacgtcctgg ctttcaggta aaccaataag tgcgccatga ccaccaggaa caaagattgc
     1861 tgcatattcg ctatcagcgt tgaggcttgc gacaacatcc gcgagtttct tcggattgcg
     1921 gaacaacgat ttatgctgct caaagaatgg cattactttt tcatctttgt gtggcatagc
     1981 ccagtattca aacttggtca tcagaccgga aatggtcgcc acttcgaatt cgaaacctgc
     2041 agcatggaga tgatacaacg gcagcaacgt ttcaatcgga tggttaccgg tcgagaatag
     2101 tttcccgtta tcggtcggca aataacgttc gtccgcggcg atcaccagaa ttttatgttt
     2161 accgcgatac ggttttggat agtccacgcc atcaagatca gagacgggac tggtatattg
     2221 gctaagcgaa tattctgaag ggaagaatgc attatcttca gcaatatcga cctgcgggtt
     2281 tttacttgtt tgaacagtca tagtgtattc cttattgttg cttagttagg gtagtcacta
     2341 tatttgcgac tgagctggtc gctattttta accaggctga aaaattgagt caatatatcg
     2401 aaagcggtag ttggcgggca taagtatgta atgatgatgc tgcactggtg ttgaatatta
     2461 ttcacgccaa attttcctta ttgagctacg tcgtcgcaag tggcctcaat gattttagac
     2521 cattatcaga ggtccggggc ggcagggaga gatttagtgc tgtggaatat tgtggtgctg
     2581 atgaatattt atatcgcgga ttggtagaga aaaagctttt ttcatgcact atttttgcaa
     2641 atatttctat atggacggcg gttttgttgc ggattaattc ataaagaaat gcatttggat
     2701 gttggccgga agagacgctg gtagatttta ttgagccagg aggggtgggg taaggtgctg
     2761 tatcgatatg ctttggcaat tgcgtgtgcc gcagatggag atgaaatgcc aataaaaaag
     2821 cctgagttcg atgagggacg aactcaggct ggtgttaagt tttacagtaa cacgccgcgg
     2881 cgcgttggct gattagaact ggtagacgat accaactgca aactggtcat cagttgcggc
     2941 atcggtaatc gctgcatcgt ctttatccag caggttaaat ttgtatgctg tgtagacgtt
     3001 catgttctta ttgaagtagt accaggtacc aacttcgata taatttacgc ggtcagcatc
     3061 aacaccttta tgtccatcac gagcataaag atctttgcct ttggaatata cgtagcctaa
     3121 agacggacgt aaaccgaagt cgaactgata ctgtacaaca gcttcaaagt tttgggtttt
     3181 atttgcaaaa tgatcattag ccaaaacggt catgttgcgg gtttcaccgt acatcattgc
     3241 tgcatagata ttatttgcat catacttggc tgctaatgac cacacttcag cgttatcacc
     3301 gtagccgtca ccagcttgtt cattagtacg atcagatttg ctatatgcac ctacaaaacc
     3361 aaaaccttcg atgttgtagt ctacagacaa cgcatagcca tcaccgtttt gtttcgtaac
     3421 gtcacgattg ctgttcttac cttgatattg cacagcaaaa ttcagaccat caaccagacc
     3481 aaagaaatca gagttacggt aggttgcaac accgttggta cgaccgttca tgaagttgtc
     3541 agcggacgcc caggaatcac caccccattc aactaacata tcggtataag ctgcggcatc
     3601 ataagcaaca ccgtagttac gaccatagtc gaaagatcct aactcgttat atttcaaacc
     3661 tgcgaaggtt aaacgggttt tttcctgatt agagccttcg ggcttgctgg cgtcgaggtt
     3721 gtattcaaac tgaccaaaac cagtcagttc gctggtgatt tgggtttcac ctttaacacc
     3781 gaaacgagca taggaggtat cttcgttttc gctgttatta tcatcagtat ttgaccagat
     3841 gcgttcgcca accatcttgc cgtagaaatc aactttattg ccatctttat tataaatttc
     3901 agccgcattt gctgcgccag caactaataa cgccgggacc agcattgcca gaacttttct
     3961 tttcattatg tattcccttg tgattataat cttcatgaat atatcaataa gtgccgttat
     4021 ccaaaaaaaa gcacatttgg atactattct atgaagttca ttttatttta aagattacat
     4081 g
//
LOCUS       DAZGAP010000299         4004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGAP010000299 DAZGAP010000000
VERSION     DAZGAP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.301"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..146
                     /locus_tag="ACHLAM_005019"
     CDS             <1..146
                     /locus_tag="ACHLAM_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO7856400.1"
                     /translation="APRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEM
                     MSH"
     gene            complement(816..905)
                     /locus_tag="ACHLAM_005020"
     tRNA            complement(816..905)
                     /locus_tag="ACHLAM_005020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Ser,seq:cga)
     gene            999..1796
                     /gene="mtfA"
                     /locus_tag="ACHLAM_005021"
     CDS             999..1796
                     /gene="mtfA"
                     /locus_tag="ACHLAM_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO7856401.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1897..1972
                     /locus_tag="ACHLAM_005022"
     tRNA            1897..1972
                     /locus_tag="ACHLAM_005022"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1930..1932,aa:Asn,seq:gtt)
     gene            2285..3058
                     /locus_tag="ACHLAM_005023"
                     /pseudo
     CDS             2285..3058
                     /locus_tag="ACHLAM_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            3108..>4004
                     /locus_tag="ACHLAM_005024"
     CDS             3108..>4004
                     /locus_tag="ACHLAM_005024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFO7856402.1"
                     /translation="MAGPADASQSVLKNNRSSLKGDFTESAELYLVLHDLSGHPINVS
                     EGLEFVQSGTNVSYVQISTIDYTQNLYGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIEFISAGTRPMNGTVSVNGANLPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSS
                     TASWVDVDTSGKVTFKNVGDRNAVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLS
                     QAENYCSNQVGNGYPLPRADLLSNGHMRREIGSLYGEWGDMGNYMKEADFYSMVYWSS
                     NSAGAGQQYIVSLETGTQNTYQT"
BASE COUNT         1094 a          891 c          933 g         1086 t
ORIGIN      
        1 ttgcaccacg aaaatcatct cattttcaca tttttatggg taatgattcc cagcaatctt
       61 tattgaatga aatggaaaac tggccaacgt attatcccta tcagttgagt agcgaagaag
      121 tggtcgagga gatgatgtct cattgatttt tttgcgctga cttcagttat ttaacagaac
      181 agtgcctggt agtaacaaca ataatttacc aagacaaata agtaagacaa actcagacac
      241 gctcatactt gagtcgtgtc ttttttatta tgtctgtaga aaaaaactgc gaaaaaggcg
      301 aacaataaat acacaactca gccatacaga ctggcagcgc aaatgtttcc aggagcaagt
      361 catctgtcat ggtaaagcta accagaaata taggttaatt ttggtgttta aacctttttt
      421 acttgttagt atgatacaaa ggctttcaaa aaagctggga aattcaacaa ctcttggaaa
      481 ttatggactt tgtcccgcgg ctttaatctg acggccgcgt tccttttttg gctcaattat
      541 tgaccccttc tctacgcaac ttcagttccc gccagcaact ttgcggcagt ttcataggat
      601 caatgtctaa aagaataatg gtgatcgata agaaaacgac tgaataactg tagattttcg
      661 ctcgaaacct tcctgtcagc tccatagcga atcaagtgct gaatgtcaca gtatcgaaca
      721 gaaaacagtg acgatctaac ccttcaagaa tattctacga ttgttctgtt taggaaaagc
      781 aaggcgggaa gtcgggagat aagtcattga taaagtggcg gagagagggg gatttgaacc
      841 cccggtagag ttgcccctac tccggttttc gagaccggtc cgttcagccg ctccggcatc
      901 tctccgttca gatggttgcc atgatgccag gaaatttggc attttaacag tccctgtccg
      961 tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat gattaagtgg ccctggaaag
     1021 tacaagaatc agcacatcaa actgcccttc cctggcagga agcactatcg atcccccttt
     1081 taacgtgtct gacggaacag gaacaaagca aattagtcac tcttgccgaa cgttttttac
     1141 agcaaaagcg gcttgttcct ttacagggct ttgaactgga ttcattaaga agctgccgga
     1201 tagcacttct attttgcctg cccgttctgg agttaggact ggaatggctg gatggttttc
     1261 atgaagtctt aatttatcct gcgccatttg tggtcgatga tgaatgggaa gacgatatcg
     1321 gtctggtgca taaccaacgt attgttcagt caggtcagag ctggcagcaa gggcctatcg
     1381 ttttgaactg gttggatata caagattctt ttgatgcttc tggttttaac ctgattattc
     1441 atgaagtcgc tcataagctg gacacccgta acggcgatcg cgccagcgga gttcccttta
     1501 tttcgttgcg tgaggttgct ggctgggaac acgatcttca tgctgcaatg aacaacattc
     1561 aggaagaaat cgaattagtt ggtgagaatg cggcgagcat tgatgcttat gctgccagtg
     1621 atcctgctga atgttttgcc gtactttctg aatatttctt tagcgcccca gaactttttg
     1681 ctcctcgttt cccttcattg tggcaacgtt tctgtcaatt ttatcaacaa gatcctttgc
     1741 agagactgca tcacgctaat gatacagact cgttttcggc gacgaatgtt cattaattaa
     1801 caactttgca gattaattaa ccaattgaaa tggcttatga aatttagtgt tgacagacaa
     1861 ggtaccgcta agtaatatgc gccccgttct cacgattcct ctgtagttca gtcggtagaa
     1921 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattctaa
     1981 aaattcgctt tttagcgcaa tgtcactgac cttagttgag cattgctttt taacggatag
     2041 cgggtttttg acatcttaag cgccctcgac ctttatggtt gagggcgttt tgctatgaac
     2101 gccatcacca ttttcccctc gattacaaaa cttgagtaat tcagtagtct cccctcttgc
     2161 aactcacacc caaaactgct taacgaaaag ttattaattt tcaatcatat tgctatcagg
     2221 atttacattt tttcgctgtg ctagaaaggg cgcatttatg ttagctcgtt cagggaaggt
     2281 aagcatggct acgaagaaga gaagtggaga agaaataaat gaccaacaaa tcttatgcgg
     2341 gatgggtatt aaactacgcc gcttaactgc gggtatctgc ctggtaactc aacttgtgtt
     2401 ccctatggct gcggcagcac aaggtgtggt aaacgccgca acccaacaac cagttcctgc
     2461 acaaatcgcc attgcaaatg ccaatacggt gccctatacc cttggagcgc tggaatcggc
     2521 ccaaagcgtt gccgaacgtt tcggtatttc gttggctgag ttacgcaaac tcaaccagtt
     2581 tcgtacgttt gctcgaggtt ttgataatgt ccgccagggt gatgaactgg atgtcccggc
     2641 acaagttagt gaaaaaaaat taaccccgcc gccgggtaat agcagtgaca accttgagca
     2701 acagatagcc agtacttcac agcaaatcgg gtctctgctc gccgaagata tgaacagcga
     2761 gcaagcggcc aatatggcgc gtggatgggc ctcttctcag gcatctggcg cattgacaga
     2821 ctggttaagc cgcttcggta ccgcaagaat cacgctgggc gtggatgaag attttagcct
     2881 gaagaattcc cagttcgatt ttctccatcc atggtatgaa acgcctgata atctcttttt
     2941 cagccagcat accctccacc gtactgacga acgtacgcag attaacaacg gcttgggttg
     3001 gcgtcatttc actcccacat ggatgtcggg catcaacttc tttttcgacc acgatcttaa
     3061 gcgcagaaac gagctatggt ggggcgcaaa cagtagatat aacgctggtg gcaggcccgg
     3121 cagacgcctc gcagtccgtc cttaaaaaca accggtcatc actgaaaggg gactttaccg
     3181 aaagtgctga actatatctt gttttacacg atctatcagg tcatccgatc aatgtttctg
     3241 aagggctgga atttgtgcaa tcaggtacca acgtgtccta tgtgcaaatc agcacaattg
     3301 attacactca aaatttatac ggtgagtata aagccactgt tacaggcggc ggagagggta
     3361 tcgcaacgct gatccccgtg ttgaatggtg ttcaccaggc gggtctgagt accacgatag
     3421 aattcattag cgcgggaacc agaccaatga acggtacagt atcagtgaat ggtgcaaacc
     3481 tacctgcagc ttcattccct tcgcagggat ttaccggggc gtattatcag ttgaataatg
     3541 acaactttgc accaggaaaa acggcggctg attatgcgtt ttcaagcact gcctcctggg
     3601 ttgatgttga tacttccggt aaggtgacat tcaaaaatgt tggagataga aatgctgtaa
     3661 taatcacggc gaccccaaga tcaggtggcg ctatttacca aacgcaagta cgggtgaagg
     3721 gctggtgggt gaatcacggt aataacctca tgcaattaag ccaggctgaa aactactgca
     3781 gcaaccaggt cggtaatggc tacccccttc cccgcgcgga ccttttaagc aatgggcaca
     3841 tgcggcgcga aatagggtca ctgtatggtg agtggggaga tatgggaaat tacatgaagg
     3901 aagctgattt ttattcaatg gtatattggt cttctaattc cgcaggcgca ggccaacaat
     3961 acattgtatc cctggaaaca gggacgcaaa acacttatca aacg
//
LOCUS       DAZGAP010000300         4002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGAP010000300 DAZGAP010000000
VERSION     DAZGAP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.302"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..480
                     /locus_tag="ACHLAM_005025"
     CDS             <1..480
                     /locus_tag="ACHLAM_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7856403.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            496..1833
                     /gene="tssK"
                     /locus_tag="ACHLAM_005026"
     CDS             496..1833
                     /gene="tssK"
                     /locus_tag="ACHLAM_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7856404.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCKDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            1830..2483
                     /gene="tssL"
                     /locus_tag="ACHLAM_005027"
     CDS             1830..2483
                     /gene="tssL"
                     /locus_tag="ACHLAM_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO7856405.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            2486..>4002
                     /locus_tag="ACHLAM_005028"
     CDS             2486..>4002
                     /locus_tag="ACHLAM_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7856406.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWYCLSSRRDAPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVR"
BASE COUNT          797 a         1026 c         1214 g          965 t
ORIGIN      
        1 ccggaaactc gggagtttga attcgctaat ctgggcttta ttccgctctc ttactacaag
       61 aaccgggatt atgcctgctt cttctccgct aactccaccc agaaaccggc actgtacgat
      121 acgccggatg caacggcgaa cagccgtatt aatgcccgtc ttccctatat cttcctgctg
      181 tcccgcattg cgcattacct gaagatcatt cagagggaga atatcggtac caccaaagat
      241 cgccgtctgc ttgaactgga actgaataac tggatccggg gtctggtgac ggagatgacc
      301 gatccgggtg atgaactgca ggcctcacat ccgctgcgtg acggaaaagt ggtggtggaa
      361 gacatcgaag acaacccggg atttttccgc gtgaaactct atgctgtacc acatttccag
      421 gtggaaggta tggacgtcag tctgtcactg gtttcgcaga tgccgaaggc aaaagcgtaa
      481 ggcaggcgtc gggaaatgaa gatttatcgt ccactgtggg aagacggggc ttttctgatg
      541 ccccagcagt ttcagcagca ggctgcctgg gatgttcatc tggcagacag cgttgcccga
      601 atggggctgg cgcatccctg gggcgtggtg gctgcagagt ttgatgactc tctgttaccg
      661 ctttcacggt tgaatgccac ccgtctgatt gttcgtttcc cggacggaac gcttattgat
      721 acggaacgtg cggataatct gcctccggtc tgtgatttat cgacggtttc tgagcgttcc
      781 ctggtggata ttgtgctggc gctaccgtta ctgaatgcta acggtggcaa ccttgatgat
      841 ggcagtgaga gcgagcgtcc gcgtcgctgg aaatcagaaa gggtgaatgt gcaggagctg
      901 gccgggcatg agcaaagcga agtggcggta ttgcggcata acctgactct gcgcatggct
      961 catcaggaaa atgctgcctg gctgacctgt cctgttaccc gtcttgttcg agatgcacag
     1021 ggacagtggt gcaaggaccc gcgttttatt ccccctctgc tgacgctgtc tgccagtcct
     1081 tcgctgatga ctgagcttgc ggagttgctt catcacctgc aggcgcgtcg ccagcgactg
     1141 atgtccatgc gtcgggagaa caatgcccgt cttgcggatt ttgcagtggc ggacgtgtcg
     1201 ttgttctggt tgctgaatgc cctgaacagt gcggagcctg ttctgaaaga gttgcttgac
     1261 atgccttacc gccacccgga actgttgtac agggagcttg cccggctggc aggctccctg
     1321 ctcacattct cgcttgagca taatgtggat gcggttccgg cataccgtca tgaaacacct
     1381 gaaaatgtgt ttccaccctt gctttctttg ctgaaccagc tgctggaggc aagcctgcct
     1441 tcccgtgtgg ttttcattga actgaaacaa aatggtgtga tgtgggaggg ggcattacat
     1501 gatgcgcgtt tgcgcgaagg ggcggatttc tggctttcag tgcgctcttc catacccggg
     1561 catgaactgc agacgaagtt tccgcagcta tgcaaagccg gaagccccga tgatgtgtct
     1621 gaggtggtga atgtggcgct gagcggggtc attatccgtc cggttacgca tgtaccggcg
     1681 gccattccgc tccggctgga aaaccagtat ttcgcgctgg atttaagtac ggatgcagcc
     1741 cgggcgatgc tggacgcggg gcgttgtact ttctacaccc cggcatcgct gggggatgtg
     1801 aaactggaac tttttgcggt actgcggaca tgaataaacc tgttatctcc cgggctgaac
     1861 agatttttta tcccggctgg ctgatggtca gccagctgcg ctgcggacaa ccggttgagg
     1921 atggtaaggc cctgtatcgc cgtgcctgtc agctggtgac gcaggcgcgt gaagagctgg
     1981 ctgaagcaga attcagtcag gaaagcagtg aaatcatgtt gtatgccttc tgcgccctgc
     2041 tggacgagag tgtgctgaac cgtgaaaaaa cagacgatgg ctggcacacc tggcagcagg
     2101 acccgctgca ggctcatttt tttggtacgc tcaatgccgg agaagagctc tgggagcgta
     2161 ttcgtgagca actgaaatta cctgcgccgg atgtggcggt actgacatgc ctctgccgta
     2221 cgcttcagct cggttttacc ggtcagtacc ggtcgcagga tgatgagcgt cgcgaagatg
     2281 taattcgcgc actgacagcg cgtgttcctg catttacttt cgcacaggat gcgccggtgg
     2341 tggtgcgtgc accgggttac cgtgctggca gaacgatgta ctggttgtca tgggtggcgg
     2401 gtattgtgac gctggctggt ctctggtgtg tgctttcttc tgtgctcgca gaccaggtgg
     2461 cacggcttac cgggcaggga taaccatgca cgtacgttcc cgtctctggc tgacaatact
     2521 gagtctgttc ctgacactgt ggctgatttc gggattcctg ccactgtctg cagtaaacca
     2581 gataatactg accctggttt cagttgtggc ggcgggactg gtgctgtggt accagtggta
     2641 ttgtctgtct tcccgtcgtg atgccccgga gaaggctggc gatgacggtc tgccgcctga
     2701 atattttcag ggacatgtgc tgcttgttgc cggaaactca gatgcgtggt tcgaacccgg
     2761 tcaggcatac cgggaaacag catcgggatg gtatcttcgt gtggatacgc cggagcagat
     2821 gagaatactg gcagagcggt tgtcgtacag ccgtccggcc ctgtgtggcc aggtcgtggt
     2881 catgctggga cttttaccgg agcagcatac ctcagatgaa gctttcctgt cgtggttgcg
     2941 gaactggcag cgtgcagtca tgcagtgccg gcagtggctg aataccatgc cgccggtgct
     3001 ggtgacattg atggtttcag ggccgtcacc acaaaacaca tccttaaata agcactctgc
     3061 acactggttt acactgacac cggacctgtc gggaatgcac atacgcctga cggaagccgg
     3121 agccattccg gcagaggaat ggctccggga gtgtgatgca gcgtccaggc tccggtgcgt
     3181 cagtgaactg ttatggctga atgccatgtt gtcctggtac aaccgggtta ctgatgccct
     3241 gacagatatt tgccggcagg agcactttta tttccggccg gttgccgttg gattctgtct
     3301 gacgtcggtt gcggcccggc ccggtaatct ctggcaacaa caactgacgt cactcactgt
     3361 actgccaccg cctgccggcg tgtcatcaca ggcactgccg ttgccggata ttctgctgtc
     3421 cggtattccc cgtcatcgtg gactcagtca ctggcacaga ctttgtctcc agacgggggc
     3481 gattgtcggc gtttttcttc tgctggctat gctggcgtct tttatgaata accagcgact
     3541 ggttcgcagc gtgggggaac acctgacggc ttggcaccgg atgcgcggat attcatctga
     3601 atcgaaaaaa actgcactgc aacgtcttca ggctgacagc cagttactga acgactggca
     3661 gcaacgtggt gtgccgcagc gttacggact gggattgtat cagggaatgt ggctgatacc
     3721 gccggttgag cgagcgactg gtgagtggat tgcaccactg cctccccgtc cggtaataca
     3781 gaaagttact cagacgccga aaatggttcg ccttaacagc ctggcgctgt ttgatgccgg
     3841 taaatggacg ctgaaaccgg gcgccacaaa atggctggtg aatgccctgg tggatattaa
     3901 ggcaaaagcg ggctggctga ttgtggtcag tggtcacacg gacaatacgg gcgacccgca
     3961 gcgcaaccag gccctgtcac tgaagcgcgc agaggccgtt cg
//
LOCUS       DAZGAP010000301         3999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGAP010000301 DAZGAP010000000
VERSION     DAZGAP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.303"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..666
                     /locus_tag="ACHLAM_005029"
     CDS             58..666
                     /locus_tag="ACHLAM_005029"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO7856407.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            764..2155
                     /gene="selA"
                     /locus_tag="ACHLAM_005030"
     CDS             764..2155
                     /gene="selA"
                     /locus_tag="ACHLAM_005030"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO7856408.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2152..3996
                     /gene="selB"
                     /locus_tag="ACHLAM_005031"
     CDS             2152..3996
                     /gene="selB"
                     /locus_tag="ACHLAM_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO7856409.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          886 a         1021 c         1197 g          895 t
ORIGIN      
        1 gccctttttc ctatcaccag gccatactga tatttttcct cgcacaaagg actcggcatg
       61 aaactcgtcg gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa
      121 aagggcataa ctttcgaatt tattaatgaa ctgccctata acgcggataa cggcgtggcg
      181 caatttaacc cgttagggaa agtgccggtg ctggtgaccg aagagggcga atgctggttt
      241 gattcgccga tcatcgctga atatattgaa ttaatgaatg tcgctccggc gatgttgccg
      301 cgcgatccgc tggagtcgtt gcgggtgcgc aaaattgagg cactggcgga tggcattatg
      361 gatgccgggc tggtatcggt gcgtgaacag gcgcgtccag cggcgcagca gtctgaagat
      421 gaattgttac gccagcgcga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg
      481 gtcgatggca cgctcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgcc
      541 gtcggatatc tcaatttccg ccgcgttgcg ccaggctggt gtgtcgaccg cccacactta
      601 gtcaaactgg tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag
      661 gcttgatgcg cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa
      721 cgcccttctc cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg
      781 ttccctctat agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc
      841 tttgcgtgat acttatggtc acacccgcgt ggtggaattg ctgcgtcaga tgcttgacga
      901 agcgcgagaa gtgattcgtg atagccagac gctgcccgca tggtgtgaaa actgggcgca
      961 agaagtcgat gcccggttga cgaaagaggc gcagagcgcg ctgcgtccgg tgatcaacct
     1021 gacgggaacc gtgctgcata ccaatcttgg gcgagcttta caggcagaag ccgcggtgga
     1081 agccgttgcg caggctatgc gttcgccagt gaccctcgag tacgatctgg acgacgccgg
     1141 acgcggacat cgcgatcggg cgctggcgca gctgctgtgc cgcattacgg gagcggaaga
     1201 tgcctgtatc gttaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag
     1261 cgggaaagag gtggtggtat ctcgcggcga actggtggag attggcggcg cgtttcgtat
     1321 tcctgatgtc atgcgtcagg caggctgcac cctacacgaa gtggggacca ccaaccgcac
     1381 gcacgctaat gattatcgtc aggcggtgaa tgaaaatacc gcgctgttga tgaaagtaca
     1441 taccagtaac tacagcattc agggattcac caaagcgata gatgaagcgg aactggtggc
     1501 gctcggcaaa gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggttga
     1561 tcttagccag tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt
     1621 cagtctggtg agtttctccg gtgacaagtt gttaggcggg ccgcaggcag gaattattgt
     1681 tggtaaaaaa gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc
     1741 ggataaaatg accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc
     1801 tctgagtgaa aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat
     1861 ccaggcacaa cgtttacagg ctccccttgc cgcacattac ggcgcggagt ttgcagtcca
     1921 ggttatgcca tgtctttcgc agattggcag tggttcactg ccggttgatc gcctgccgag
     1981 cgcggcatta acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc
     2041 ccgctggcgt gaattgccag tgccggtgat tggtcgtatt tatgacggac gattgtggct
     2101 ggatttacgc tgccttgaag atgagcaacg gtttttggag atgttgttga aatgattatt
     2161 gcgactgccg gacacgttga ccacggcaaa acaaccttat tgcaggcgat tactggcgta
     2221 aatgccgatc gtctgccgga agaaaaaaag cgcggcatga ctatcgatct cggctatgcc
     2281 tactggccgc agccggatgg ccgcgtgcct ggttttatcg acgttcccgg tcatgaaaag
     2341 tttctttcca acatgctggc gggcgtcggt ggtatcgatc acgcgctgtt ggtggtggcg
     2401 tgcgatgacg gtgtgatggc acagacccgt gagcatctgg cgattttgca gctgactggt
     2461 aacccgatgc tgacagtggc gctgaccaaa gccgatcgcg tggacgaagc gcgtgttgat
     2521 gaggttgaac gccaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg
     2581 tttatcaccg cagcaaccga aggtcgggga attgatgccc tgcgcgagca tctgcttcag
     2641 ttaccagaac gcgaacacgc cagccaacat agtttccgcc tcgcgatcga ccgtgcattt
     2701 accgtaaaag gtgccgggct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta
     2761 ggcgattcac tctggctgac tggtgtaaat aaaccgatgc gcgtgcgtgc gctgcatgcg
     2821 caaaatcagc caacagaaac tgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt
     2881 gatgcggaaa aagagcagat taaccgtggc gactggctgc ttgccgatgc gccgccggag
     2941 ccgttcacac gggtgattgt cgagcttcaa acccatacac cgctgaccca gtggcagccg
     3001 ctgcatattc accacgccgc cagccacgtc acgggacgcg tttcgctgct ggaagataac
     3061 cttgccgagc tggtgttcga cacgccgctg tggctggcgg ataacgatcg cttagtgttg
     3121 cgcgatatct cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg
     3181 cgtcgcggta agcgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag
     3241 agcgatgccg atgcgttatc tgttcatctg gaacgcggag cggttaacct tgcggatttc
     3301 gcctgggcac ggcagctcaa cggcgaaggg atgcgcgaac tgctgcaaca gcctggctat
     3361 attcaggctg gttatagctt gttgaatgcg ccggttgccg ctcgctggca gcggaaaatt
     3421 ctcgacacat tagcgactta tcatgagcaa catcgcgatg aacctggtcc tgggcgagaa
     3481 cgtctgcgac gtatggcgtt gccaatggaa gatgaagcgc tggtactgtt gctgattgaa
     3541 aagatgcgcg aaagcggcga catcctcagt catcacggct ggctgcatct gccggatcac
     3601 aaagcgggct tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc actgtttggt
     3661 gacgaaccgt ggtgggtgcg agacctggca aaagaaacgg gaaccgacga gcaggcaatg
     3721 cgcctgacgc tacgccaggc ggcgcaacag gggataatta ctgcgatcgt caaagatcgt
     3781 tattaccgta acgatcggat tgtcgaattt gccaatatga tccgcgatct cgatcaggaa
     3841 tgtggttcaa cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg aaagctggca
     3901 attcaaatcc tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat
     3961 ttattgcgcg acgcattatt atttccggaa aaataagga
//
LOCUS       DAZGAP010000302         3990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGAP010000302 DAZGAP010000000
VERSION     DAZGAP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.304"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..927
                     /locus_tag="ACHLAM_005032"
     CDS             <1..927
                     /locus_tag="ACHLAM_005032"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7856410.1"
                     /translation="GEKMKYMPKLQQIKFFNEIVLCGSLRAAARKMNISQPALSRTIK
                     ELENVLGCNLLIRTRDGIVLTEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAF
                     GISSLFGVTILNNVLDNFRMKYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMP
                     VGDFIVVELFNAPFTIVARKHHPLRNCTSIDELATARWVFPETQMGYYHYISRLIPFG
                     HPDEPYAPIFTDSVVCIMNLVLYDDYITVLSRARLQEARFKEILCSIPVKYQLPVSQY
                     GLIYPRKRLMTAPAQIMIEYFQQEIQRYDWNG"
     gene            complement(1176..1343)
                     /locus_tag="ACHLAM_005033"
     CDS             complement(1176..1343)
                     /locus_tag="ACHLAM_005033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856411.1"
                     /translation="MHASPVMTMVVCPTVLLCQNGHQKDQYRESLLSQMIASGHKELT
                     RVWQMNEIQAM"
     gene            1492..2325
                     /gene="iolR"
                     /locus_tag="ACHLAM_005034"
     CDS             1492..2325
                     /gene="iolR"
                     /locus_tag="ACHLAM_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFO7856412.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASMCLAQTLAVSLALAA
                     E"
     gene            complement(2378..3811)
                     /gene="iolT1"
                     /locus_tag="ACHLAM_005035"
     CDS             complement(2378..3811)
                     /gene="iolT1"
                     /locus_tag="ACHLAM_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFO7856413.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT         1194 a          808 c          878 g         1110 t
ORIGIN      
        1 ggcgaaaaaa tgaaatatat gcctaaactt caacaaatta aatttttcaa tgaaatagtc
       61 ctgtgcggaa gtttaagagc tgctgcccgt aaaatgaata tatctcagcc agcattaagc
      121 agaacgataa aagaactgga gaatgtatta ggatgtaacc ttctgattcg aacaagggat
      181 ggtatcgttt taacagaagc tggaaagata ttctcggtcc attcacgtat gattcttgaa
      241 gagattgaaa ctgcagctct tgaagttgcg aaagtaaata tagataacac aggaaaagta
      301 gcttttggaa tttcttcgct gtttggcgta acaatactga ataatgtact tgataacttc
      361 cggatgaaat atccgaaaaa gcgtgtgatg ataagagagg gacagttgtc ctcccttatc
      421 cctttcctga gagaaggtgt tctggatttt gcaatgggta cattaacaaa agacatgcca
      481 gttggagatt ttattgtggt tgaattgttt aacgcaccgt tcactatagt ggcaagaaaa
      541 caccatcctt taaggaactg tacatcaatc gatgaacttg caactgcaag atgggtattc
      601 ccggaaactc aaatgggata ctatcattac atatcccgtc ttattccctt tggacatccg
      661 gatgaaccat atgctccaat atttaccgat tcggtagttt gtattatgaa tcttgttctc
      721 tatgatgact atataacggt tctctccaga gcaaggttac aggaagcaag attcaaagaa
      781 atattgtgtt caattcctgt taagtatcaa ctacccgtta gccagtacgg gcttatttat
      841 ccacgaaaaa ggctaatgac ggcaccagca cagataatga ttgaatattt tcaacaagag
      901 atacaacgat atgactggaa tggatgagca atacccggaa tgatggtgta caccgggata
      961 acaatttatt cggatgtgcc aatcccacgg ttgtaatggg caatataatt gttagccgat
     1021 tcgctaatgg cagggaaaag cgcggcataa atataagccc acacacatgt aacgtactgc
     1081 agatactcgc ttggcagagt tgttacttct tgggaggcag taccagccat cagatattcc
     1141 cgttccatgc caaaacctga gttacccgga taccattaca ttgcctgtat ttcattcatc
     1201 tgccacacac gtgtcagctc tttgtgacct gacgcaatca tctgactcag cagcgattca
     1261 cggtactggt ctttttggtg accgttctga cagagcaata cagtgggaca aacgaccatc
     1321 gtcatcactg gagaggcgtg catggaaact cgtcaacaac tcaagttcgc cggaacggaa
     1381 cgttaacaat agaactttac tgtataaatt tgaatactgc taaatgtatg aatggttcat
     1441 tcatgttaat attgtgattt gcaaaaattt cattttatta gagtaaaata catgtctaaa
     1501 caccaaacac agctttcctt actgcaggac gacatccgca gtcgctatga cagtctcagc
     1561 aaacgtctta aacaagttgc tcagtatatt ctggataaca gtaatagcgt tgcttttgat
     1621 acggttgctt ctattgcgca gcacgcggag gtacctcctt cgacattaat tcgttttgcc
     1681 aatgcgtttg gttttagcgg attcaatgaa atgaaacaag tatttcgcca gcatttgatg
     1741 gaggaaaccg ttagctatac agaacgggct cgtctgttca gacaaaaaaa catcaatgat
     1801 ggggaaccca caccggaaaa accgggcgaa attctgaagc tattttccat ggtaaatgca
     1861 caggtactac aacaattacc gatgcagatt aaaaatgagc aactggatgc tgcagtaagt
     1921 ttattgtcaa aagccgataa tatttatgtg attggattac gtcgttcatt tagcgtggcc
     1981 tgttatttaa cctatgcatt gcgccatctt gagaggcgtg catttttaat tgatggtatt
     2041 ggaggcatgt tttctgagca actgagtatg atcagctcta acgatgtagt catcgccatc
     2101 agttattcgc catacgctca ggaggttgtt gaactggttg agttgggggc aaaacgtgat
     2161 gcacatcata tcgtcattac cgacagtcag gtcagtccgt tggctgcgtt cagtgaggtc
     2221 tgttttgtaa tacgtgaagc gcaggtggat ggcttccgtt cgcaggtagc gtcgatgtgt
     2281 ctggcgcaga cgttggccgt ctcactggct ctggctgctg aataagagga agatgccgat
     2341 aatctgtttt tcatgatcat cggcattttg gctaagctta atctgaagag cgttcagcct
     2401 gaataggttg tggcttgttc ctgcgttttg tcagcacgat atcctccatt ttctctagtg
     2461 agacaccttt ggtttccggc aggtagcggc aaataaagaa atagctaaag acacagcagg
     2521 ctgcgaatat ccacattgga aatgcaccgt ggaaatgggc cagtaaccag ggattttcgt
     2581 taattatagg aaagaactgt gaaaccataa agtttgctat ccacataaat cctacagaaa
     2641 tactcattcc ctgtgaacgc atgcggttgg ggaatatttc tgatatgagc acccatgctc
     2701 caacgcccca ggaaagtgca tagaaaatca taaagaacag cataccaaag agagcataat
     2761 agccggttgt ttggctgtac agagcccatg aagttaataa taaaccaatg atgctaccaa
     2821 tggtgccttt tcgcattagc gataaacgcc ccattttgtc catgatcatt gcgccaataa
     2881 ttgatccgac gagttgaatt acgccaatcc agatggtctg aaacagcgct tcctgggcgc
     2941 taccggtaac atctctcaat acaataggtg cgtaatacat cataacgtta acgccggtta
     3001 cttgctgtaa cattgcgatc atgcaaccga ggaaaaggat aaaacgtacg ttactgtcat
     3061 ggtaattgag cttttggtgg gccgtcagcc ggtcattttg cagagaggtt tttatatctg
     3121 ttagcaaatg tcgtgcatgt tcctcgttag atatctttgt aagaattttt agcgtttctt
     3181 cttcacgacc agccatcatc atccagcggg gggattccgg tataaagaat actaaaatac
     3241 agaacagaat gcatggggca atgccggcag caaacatata acgccagcca atctcaatca
     3301 gccatgtatt ggtcgcaatc gaggcgattt tatagttgac gtaaaatatg aggatctgac
     3361 caaaaacgat ggcaaactgt tgcatactca atgccctgcc acgcatgtcc ttaggtgaaa
     3421 cctccgacat atacattgga gatactgttg ctgcgaggcc gacagcaagt ccacctacga
     3481 tacgataaat aataaagtgc aggaaggtgt aggaaagaga tgttcccact gctgagattg
     3541 tgaacagcag ggcggaaatc atgagtgatt tcttgcgacc aaaacgtttg gataagtaac
     3601 ctgagctgaa tgatcccacc acacaaccta caacgacgct ggaaaccgcc cagccggttt
     3661 cggccggaga taggtgaaaa taactggtga gagaactgat tgcacctgaa ataacggcag
     3721 tatcgtagcc aaataatatc ccgcctaatg ctgcaataga acagatacgt aatatatagc
     3781 ctgtattgta acgattcact gatatgggca tagctgatta tctcctgagc agaaatcatc
     3841 tatctatttg acctataatg aaaaaattgt gtttggtcat ttccgtagct ctgcagttgt
     3901 acttcttgct ttaccatcgg taattacatt tcaatattct gattggtaat tttcactaag
     3961 atcaataatg aaacatttat tttgttatgt
//
LOCUS       DAZGAP010000303         3976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGAP010000303 DAZGAP010000000
VERSION     DAZGAP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.305"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /gene="radC"
                     /locus_tag="ACHLAM_005036"
     CDS             <1..354
                     /gene="radC"
                     /locus_tag="ACHLAM_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7856414.1"
                     /translation="AAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTE
                     VHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLI
                     VGGNQVFSFAEHGLL"
     gene            417..638
                     /locus_tag="ACHLAM_005037"
     CDS             417..638
                     /locus_tag="ACHLAM_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7856415.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            801..1175
                     /locus_tag="ACHLAM_005038"
     CDS             801..1175
                     /locus_tag="ACHLAM_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7856416.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            1222..1599
                     /locus_tag="ACHLAM_005039"
     CDS             1222..1599
                     /locus_tag="ACHLAM_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7856417.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            1596..2084
                     /locus_tag="ACHLAM_005040"
     CDS             1596..2084
                     /locus_tag="ACHLAM_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7856418.1"
                     /translation="MKLTLTLEADNVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDCETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            2096..2293
                     /locus_tag="ACHLAM_005041"
     CDS             2096..2293
                     /locus_tag="ACHLAM_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7856419.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLHQNR"
     gene            2378..3223
                     /locus_tag="ACHLAM_005042"
     CDS             2378..3223
                     /locus_tag="ACHLAM_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7856420.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDWWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKECYEDEMFSIRYFKKGSAHITFKNQELVDRLNDIIARHYPR
                     MLPSL"
BASE COUNT         1047 a         1030 c          928 g          971 t
ORIGIN      
        1 gcggcacggg aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg
       61 ctgtatctga ataaccagaa tcagttgatt gccggtgaaa ccctcttcac cggcaccatc
      121 aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg ccctgtacca caatgccgct
      181 gccgtggtgc tggcgcacaa tcacccgtcc ggtgaagtca cacccagcaa ggcagaccgc
      241 cttatcacgg aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat
      301 ctgatagtcg gtggcaacca ggttttctcc tttgccgaac atggtctgct ttaacccgtc
      361 acaaccacat cacgcttgtt ttcactttta tcttctgtct tcagaggtat cacactatga
      421 aaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca acatccggca tcccgtcttt
      481 ttcctttctg taccggcaaa taccgctggc acggcagcac tgaagcgtat accggtcgtg
      541 aagtgcagga tattcccggt gtgctggccg tgtttgctga acgccataag gacagttttg
      601 gcccgtatgt ccggctgatg agcgtcaccc tgaactgatg ttccgtcacc cggaaaacga
      661 gtacgaccag atggactcag acgccgctgt ggatgaggcc ctgctcatca acgaatacac
      721 ctttaccgaa gcaggtcagc gcgccggctg acctgtcatc gttaaaccat tcttaaccac
      781 ttttcagaga ggattttatc gtgtcagaca aactctccgg gataactcat cccgacgaca
      841 atcacgaccg cccctggtgg gggcttccct gcacagtgag gccctgtttt ggtgcccgtc
      901 tggtgcagga gggtaaccgc ctgcattacc ttgccgaccg cgccggtatc agaggccgct
      961 tcagcgacgc agatgcatac catctggacc aggcctttcc gctgctgatg aaacaactgg
     1021 agctcatgct caccagcggt gaactgaatc cccgaaatca gcataccgtc acgctgtatg
     1081 caaaagggct gacctgcgaa gccgacaccc tcggctcctg tggttacgtt tatctggctg
     1141 tttatccggc acaggcaccc gcaaccacct cataaccccg aagctctcac ctttagcact
     1201 accagttaca gagaacttaa gatgaaaaca ttatccgaca cacatgtacg ggaggtatct
     1261 cgctgcccgt ctcccgtcac catctggcag acactgctca tccgactgct ggaccagcat
     1321 tatggcctca cactgaatga cacaccgttt gccgatgaac gtgtgattga gcagcatatt
     1381 gaagctggca tttcactgtg cgatgcagtg aactttcttg ttgaaaaata cgcactggtg
     1441 cgtaccgacc agccggaatt cagtgcctgt acccgttctc agttaataaa cagcatcgat
     1501 atcctccggg ctcgcagggc gaccggcctg atgacccgcg acaattacag aacggtaaat
     1561 aacattaccc aggggaagca tccggaggcg aaacaatgaa actgactctg acgctggaag
     1621 cagacaacgt taacgtgcag gcgctgaaca tgggacgaat tgtcgttgac gtcgatggtg
     1681 ttaatctcgc tgaactgatt aacgtggtct gcgacaatgg ccactccctt cgtgttgttg
     1741 atgaatctga ccgggtctca acagactgca cgccaccatt tgctgccctt accggcatac
     1801 gctgcagtac cgcacatatc acggaaacgg acaacgcctg gctgtactcg ctgtcacacc
     1861 agactaatga cgactgcgaa acagaatgga ttcattttac aggcagcgga tatctgttac
     1921 gtaccgatgc gtggtcatac ccggttctgc ggcttaagcg cctgggcctg tcaaaaacgt
     1981 tccgtcgtct ggttgtcaca cttacccgac gttatggcgt cagtctcatt catctggatg
     2041 ccagcgctga atgcctgccg ggtttaccca ctttcaactg gtaaccagga acaacatgaa
     2101 atcattaacc acggaaacag cactggatat tctgattgcg tggctgcagg acaatatcga
     2161 ctgcgaatcg ggaattatct ttgacaacga tgaggataaa acggattcgg cagcactgct
     2221 gccctgcatt gaacaggcga gggaggatat ccataccctg cgccaggtgc agcttctgca
     2281 ccagaaccgg tgagtcttac tcaccagact tcattccact gacgccagcc tgaacagggc
     2341 tggcgttttc atttatctgc gaaaaggaat atcgattatg tctgcaatca cagtctcccg
     2401 tccggaagtg gtcaccgggc atacggacgt tatctgctcc acctcagtca gtcacattct
     2461 ggctgtgcga aagactacac tgctgcaaat cgacacactt atccggcaac tggctgaaat
     2521 ctcggtactg acagaaagta ttggaggtaa aactgctctg gactgggcca tgaaacagga
     2581 ttttcgctgc ggctgctggc tgatggagaa acctgaaacc gcaatgaaag ctatcacacg
     2641 caatctcgat cgtgaaatct ggcgtgacct gatgcaacgt tccgggatgc tttccttaat
     2701 ggatgcacag gcccgtgata catggtaccg gtcactggaa tatgataatt ttccggaaat
     2761 cagcgaggcg aatatcctga gcacatttga acaactgcac cagaataagg atgaggtgtt
     2821 tgagcgaggg gtgatcaacg tcttcagggg actgagctgg aattacaaaa ccaattgccc
     2881 ctgcaaattt ggcagtaaaa ttatcgtcaa caacctggtg aggtgggact ggtgggggtt
     2941 tcatcttatc accgggcaac aggcagatcg acttgccgat ctggaaagaa tgctacacct
     3001 gttcagcggc aaaccgatcc ccgacaaccg ggaaaacatc acccttcgtc tggatgatca
     3061 catccagtct gttcagggta aagagtgcta tgaagatgag atgttcagca tcagatactt
     3121 taagaagggc tccgcgcaca tcacgttcaa aaatcaagaa ctggttgaca ggctgaatga
     3181 cattattgca agacactatc cgagaatgtt accttcatta taaagttcta agaatgaatt
     3241 caattttgca ttttagcttc attccctcac gcaaaattat ttgggggttc ttatgggggt
     3301 tactcattta tccaacacaa attaatcatt taaaattaat atattaatac tcatattcga
     3361 ctcctgtgat cgccaaaatg cctcttcagt gatccccatc ctgcttattt ctgagcaacc
     3421 tgaccctgct gatgtatctg gtagtatgac agagtgattc atttttcttg ccgagtgtac
     3481 gaaaacgtct tccttgccac cgactatcaa ccgtcactca aaaaaacctc tctttttttc
     3541 aagctatcaa gaataagttg cattttatct gcaccggaca ccagccatcc atatatggct
     3601 tttacctgct acaaagatga agaagtgcat aaatgtgatg ttaaaacaat caaagaaaaa
     3661 ttacatgtgc aataatttca ctataacatt ataatccatg gtgaaaataa cactgcacaa
     3721 ggattgtcat catcaaactc atcggaacta ttttttctgt cgtcattggt gtaatactga
     3781 ttgtaagtat cctttcagga gattattttt gccgtgtggg gcatggaact tgtaaagcag
     3841 ccaaaagcgc taggcattta attgaaagta aagagaaaaa cagaagtagt cgcgtcaaag
     3901 tctttagcac gcatattaat ttgagtcatc cagcggcatc ctggctagca actcaaactt
     3961 gcgctaacaa tatatt
//
LOCUS       DAZGAP010000304         3975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGAP010000304 DAZGAP010000000
VERSION     DAZGAP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.306"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            617..1660
                     /gene="subA"
                     /locus_tag="ACHLAM_005043"
     CDS             617..1660
                     /gene="subA"
                     /locus_tag="ACHLAM_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO7856421.1"
                     /translation="MLKNLRPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYSSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMNEEL"
     gene            1677..2099
                     /gene="subB"
                     /locus_tag="ACHLAM_005044"
     CDS             1677..2099
                     /gene="subB"
                     /locus_tag="ACHLAM_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO7856422.1"
                     /translation="MTIKKFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(2414..3400)
                     /locus_tag="ACHLAM_005045"
     CDS             complement(2414..3400)
                     /locus_tag="ACHLAM_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7856423.1"
                     /translation="MYRHDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            3500..>3975
                     /locus_tag="ACHLAM_005046"
     CDS             3500..>3975
                     /locus_tag="ACHLAM_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7856424.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEV"
BASE COUNT         1135 a          857 c          870 g         1113 t
ORIGIN      
        1 cgtctgaaat tggtgttgct ctagaagctg aaaaaatagg tgaaattgaa aaaataaaaa
       61 tgccgcaaat aagaaaagat gtaaattaag gaaacaacat gttgggtgga gcggacaatc
      121 caaatggcga attaccgtct tatatcactg gcgctgacac ctgatatccg gggcttactg
      181 cacacgtaat aatgagccag ggtaaacgac acaaccatgc gctgatcgct ccggtaagac
      241 ggcactgtaa tttcctgttc gccatgatgc atgacggtac tctttattgt cataaacatg
      301 ccaggcaaca tagttgcccc ctcttatttt ttctggctaa tttttaccca tcatagctgg
      361 atttacttct gctctgaagg agtaggaaga gcatctcacc gattacctat tgcagaaatc
      421 ctggtgcata ttaccgtgaa ggagttcagt gagctattga atatgcaaat aaataaatta
      481 atatttttgt gaatgacttc actcgcttta ttgattgtga ttgtttatct tttgtcgaaa
      541 acaggatatt tcagatgtga tgttcctgta acatattgac ctgcaatact gtttatttgt
      601 accactggag gatattgtgc tgaaaaattt acggccgcat attctgttcc tgctttttat
      661 atctgtgtct gtcagggcag aaaaaccctg gtattttgat gcaattggcc tgacagaaac
      721 aaccatgtcg ctcacagaca agaatacccc ggttgtcgta tctgttgttg attccggagt
      781 ggcatttgta ggaggcctca gcgacagcga atttgccaaa ttcagtttta ctcaggatgg
      841 ttcgccattc ccggtaaaag agtctgaagc tttatatatt catggtactg ccatggcttc
      901 actcattgcc tcacgtcatg gggtttatgg ggtttatcct catgcactga tatccagcag
      961 aagggttatt cctgacggtg tacaggactc atggattaga gcaactgaaa gcattatgtc
     1021 aaatgttttt cttgcaccag gagaagagaa aatcattaat atatcgggag gccagaaggg
     1081 gatatcttcc gcatcggtct ggtccgaact gctttcccgt atgggccgga ataatgagcg
     1141 attaattgtt gcggcagtgg gtaatgatgg cgcagatata cgaaaactga gtgctcagca
     1201 gagaatatgg cctgcggctt accatccggt cagcagtgtg aataaaaagc aggatcctgt
     1261 gataagagtg gcagctctgg cacagtaccg gaaaggagaa acacctgtat tgcatggtgg
     1321 aggggtcacc ggaagtcggt tcggaaacgg ctgggttgat attgccgcac cgggacagaa
     1381 tattacattc ctcagaccag atggaaaaac ggggatcggt agtggaacct cggaagcaac
     1441 agctatagtt tccggcgttc tggcagcgat ggtctcatgt aatccccgtg caacagcgac
     1501 agaactgaag cgtacactgc tggagtctgc agacaaatac tcctcacttg cagataaggt
     1561 gacggaaggg agggttctta atgcagaaaa agcaattagc atgttctgca agaaaaatta
     1621 tattcctgtc cgtcagggaa ggatgaatga agaactgtaa aacttcggag gtgaatatga
     1681 ctattaagaa gttttttgta tgtgcaggtg tcatgggatg tttatccctt aatcctgcca
     1741 tggcagagtg gactggcgat gcccgggatg gcatgttttc aggcgttgtt attgatcagt
     1801 ttcatacagg gcaaatagat aataaccctt atttttgtat tgaagggaaa caacctggtg
     1861 gttcttccat aagagcctgc tcgatgaaga attcatctgt ctgggggcct tcgttttcca
     1921 cattatataa tcaggcacta tatttttaca caacaggtca gttggtcagg atttattatg
     1981 aacccggggt atggacgtat ccaccctttg tgaaggcatt aacgtccaat gcccttgttg
     2041 ggttatcgac ctgcgccaca tcgactgaat gttttggccc cgacagaaaa aagaactaat
     2101 aggtgatgtt tccgtccaga gatactctgg atggagtgtc ggcgtcagtg atataagact
     2161 cgagtgaaca ctgagccgac ctcgaaatga cgttgtaggt gcagacatgc cataatggga
     2221 ctgtcgatga tattacaggg actcacgtcc ctggtgcgtg ccttcgtgac acactaccgt
     2281 ccacatagta tcagcaccag tgatatcaaa cggtaacgac cagctatcct ggtgttgata
     2341 ccgacaactc cataccactc cgtcatgtct ctcagagaat catgtcagag ctgtagatat
     2401 atcctgaact tgtctacaaa taatttctta ttttttgcgc ataacattca gcaataactc
     2461 cccttctggc cattgaatta aagtgtgtac ttctctgtga cgttgtccag cttaattctg
     2521 tcctccatga tgaccccaga tacccgacag ataaatcatc ggcatcttct tctggctcat
     2581 tagttaattc atgtcggtta ctatcctgaa agctgagaaa atgtatttca tcatcagtcc
     2641 gttgtctgta atgtccatat ataaaatcat attctttttg ataagattcc ttccagtacc
     2701 aggttgtatc accacacaac catggaactc tttctgacga attaccaaca cactgagatg
     2761 agtattcaga taactcagac ctgaaagaag ttaccatctg ataaaacaag tcaggatgct
     2821 tttcataatc tggtgacata agatcaaact ccccctgcat ccagcagaca gatagcagtt
     2881 tattctttct gttatattcc agagctacct taacccgggc tactaagtcc tcatagagtg
     2941 ctgttccggt gccccaccgc attgcatcag ctgttgcccc tgtatcaggt acatacatac
     3001 cttcagctcc aacagtaaag gcggcacctc ctctgcaaca aggaaccagt aatatccctg
     3061 cttgctcagg tatgtatggc agtaactttt tagctatatg caatccctgt ccgacacaac
     3121 catattcccc tttatgcaaa tcagcagccg gatggtgata tccactcata tcctgaacat
     3181 catgcaggca gtgatctgcc ggaataatct cattgaattt acactctttt cctcctggtg
     3241 tgattgtttt tcgacgagcc agttgtttta cacgggactc gggtttgtcg agtgtatctg
     3301 gcaggggaat accttcaccg taggccatac cattagactg tcctgcgagg gctatcacat
     3361 accagaaatc tattttttca ttaaaatcat gacgatacat cactccccct ttacataata
     3421 gatttaatta aatattttca ttattaaaag aaagtgttaa attaaaaact catttgctct
     3481 aatccatata aatcaatata tgtccgacaa caaaaaaacc gtgttacccg ggctaacaac
     3541 gaccagaaag cccattgtta caaaaaaact ttcagaaatc gttgccgaag agcttgaaag
     3601 aatgatccgc caggaggaga taagggaagg tgaatacctg ccttctgaac gtgaacttat
     3661 ggcattcttt ggcgttggaa gaccatctgt cagagaggct ttatcatctc tcagacataa
     3721 agggctaatc cggtataaca agggagaaaa agccagtgtc tgccgtccat caccagaaac
     3781 catcattaat gggttatcag gcattgttaa agatttttta tccacagctg atggcatcaa
     3841 ggattttgaa agactacgca tcttttttga aaccaacctt gtaagatatg cagcaaaaaa
     3901 tgcaacgcct gaggatattc acaggcttga gaaggtgctc agtatccatc ataactcact
     3961 gagaaccccc gaagt
//
LOCUS       DAZGAP010000305         3927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGAP010000305 DAZGAP010000000
VERSION     DAZGAP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.307"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..736)
                     /gene="ychE"
                     /locus_tag="ACHLAM_005047"
     CDS             complement(89..736)
                     /gene="ychE"
                     /locus_tag="ACHLAM_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO7856425.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(767..820)
                     /gene="ychE"
                     /locus_tag="ACHLAM_005048"
     CDS             complement(767..820)
                     /gene="ychE"
                     /locus_tag="ACHLAM_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO7856426.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1213..3888
                     /gene="adhE"
                     /locus_tag="ACHLAM_005049"
                     /gene_synonym="adhC"
     CDS             1213..3888
                     /gene="adhE"
                     /locus_tag="ACHLAM_005049"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO7856427.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVAYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1077 a          978 c          924 g          948 t
ORIGIN      
        1 ccttttgctt ttgataactg cgcgggaaca aagtaaaaat aatgacgaag taaacggagc
       61 atgacagctc cgtttcattt gaaaggaatt aattaagcag gccggggaaa atccccttaa
      121 taccagtaac gataaattca atccccaatg ccatcagcaa tagccccata atacgcgtaa
      181 tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca cggtgccatg cggaacaatc
      241 cccagcaaca taaagcgaac aatgcaatag ccacaaagaa accaaacaga tagctaatgc
      301 tgtgataacg cgtcccccag acgatggtag aactgatcgc ccctggcccc gccatcaacg
      361 gcaacgccaa tggcaccaca ccaatgcttt cacgcaccgc ggtttctgat ttttcttgct
      421 tgttctgttt atcctcgcca agcttgccgc tgatcatcga catcgctatc gtcaccacca
      481 ggatgccacc ggcgatacgg aatgaatcaa ttgatatacc aaaaagttgt agaatcgtgt
      541 cgccgagaaa aagcgagatc cacaagataa tggccacaga caggttggct gtaaggttcg
      601 ttttgtttcg cgctgccgct gtctgataac tggtcatgct gataaagacg ggaataatcc
      661 ctaccgggtt gaccagcgca aataacccga tgaaaaattt gaagtaaacg ggaaaatcaa
      721 aaagggtctg aatcacggtt agctccgaag caaaagccgg ataatgttag ccataaataa
      781 ggttaaaaag acgcgctgac aatacgcctt ttgacagcat ttttcacctc ctaactactt
      841 aaaattgcta tcattcgtta ttgttatctg gttgtgcaaa acatgctaat gtagccaaca
      901 aatcatacta caatttatta actgttagct ataatggcga aaagcgatgc tgaaaggtgt
      961 cagctttgca aaaatttgat ttggatcacg taatcagtac ccagaagtga gtaatcttgc
     1021 ttacgccacc tggaagtgac gcattagaga taataactct aatgtttaaa ctcttttagt
     1081 aaatcacagt gagtgtgagc gcgagtaagc ttttgatttt catcggttaa gcaaatcatc
     1141 accgcactga ctatactctc gtattcgagc agatgattta ctaaaaaagt ttaacattat
     1201 caggagagca ttatggctgt tactaatgtc gctgaactta acgcactcgt agagcgtgta
     1261 aaaaaagccc agcgtgaata tgccagtttc actcaagagc aagtagacaa aatcttccgc
     1321 gccgccgctc tggctgctgc agatgctcga atcccactcg cgaaaatggc cgttgccgaa
     1381 tccggcatgg gtatcgtcga agataaagtg atcaaaaacc actttgcttc tgaatatatc
     1441 tataacgcct ataaagatga aaaaacctgt ggtgttctgt ctgaagacga cacttttggt
     1501 accatcacta tcgctgaacc aatcggtatt atttgcggta tcgttccgac cactaacccg
     1561 acttcaactg ctatcttcaa atcgctgatc agcctgaaga cccgtaacgc cattatcttc
     1621 tccccgcacc cgcgtgcaaa agatgcaacc aacaaagcgg ctgatatcgt tctgcaggct
     1681 gctatcgctg ccggtgctcc gaaagatctg atcggctgga tcgatcaacc ttctgttgaa
     1741 ctgtctaacg cactgatgca ccacccagac atcaacctga tcctcgcgac tggtggtccg
     1801 ggcatggtta aagccgcata cagctccggt aaaccagcta ttggtgtagg cgcgggtaac
     1861 acgccagttg ttatcgatga aactgctgat atcaaacgtg cagttgcatc tgtgctgatg
     1921 tccaaaacct tcgacaacgg cgtaatctgt gcttctgaac agtctgttgt tgttgttgac
     1981 tctgtttatg acgcagtacg tgaacgtttc gcaacccacg gcggctatct gttgcagggt
     2041 aaagagctga aagctgttca ggatgttatc ctgaaaaacg gtgcgctgaa cgcggctatc
     2101 gttggtcagc cagcctataa aattgctgaa ctggcaggct tctctgtacc agaaaatacc
     2161 aagattctga tcggtgaagt gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa
     2221 ctgtccccga ctctggcaat gtaccgtgct aaagatttcg aagacgcggt agaaaaagca
     2281 gagaaactgg ttgctatggg cggcatcggt catacctctt gcctgtacac tgaccaggat
     2341 aaccaaccgg ctcgcgttgc ctacttcggt cagaaaatga aaacggcccg tattctgatc
     2401 aacaccccgg cgtctcaggg tggtatcggt gacctgtata acttcaaact cgcaccttcc
     2461 ctgactctgg gttgtggttc ctggggtggt aactccatct ctgaaaacgt tggtccgaaa
     2521 cacctgatca acaagaaaac cgttgctaag cgagctgaaa acatgttgtg gcacaaactt
     2581 ccgaaatcta tctacttccg ccgtggctct ctgccaatcg cgctggatga agtgattact
     2641 gatggccaca aacgtgcgct catcgtgact gaccgcttcc tgttcaacaa tggttatgct
     2701 gatcagatca cttccgtact gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta
     2761 gaagctgacc cgaccctgag catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa
     2821 ccagacgtga ttatcgcgct gggtggtggt tccccgatgg atgccgcgaa gatcatgtgg
     2881 gtgatgtacg agcatccaga aactcatttc gaagaactgg cgctgcgctt tatggatatc
     2941 cgtaaacgta tctacaagtt cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc
     3001 accacttctg gtacaggttc tgaagtcact ccgtttgcgg ttgtaactga cgacgctact
     3061 ggtcagaaat atccgctggc agactatgcg ctgaccccgg atatggcgat tgtcgatgcc
     3121 aacctggtta tggacatgcc gaagtccctg tgtgctttcg gtggcctgga cgcagtaact
     3181 cacgccatgg aagcttatgt ttctgtactg gcatctgagt tctctgatgg tcaggcactg
     3241 caggcactga aactgctgaa agaatatctg ccagcgtcct accacgaagg atctaaaaat
     3301 ccggtagcgc gtgaacgtgt tcacagtgca gcaactatcg cgggtatcgc gtttgcgaac
     3361 gccttcctgg gtgtatgtca ctcaatggcg cacaaactgg gttcccagtt ccatattccg
     3421 cacggtctgg caaacgccct gctgatttgt aacgttattc gctacaacgc gaatgacaac
     3481 ccgaccaagc agactgcatt cagccagtat gaccgtccgc aggctcgccg tcgttatgct
     3541 gaaattgctg accacctggg tctgagcgca ccgggcgacc gtactgctgc taagatcgag
     3601 aaactgctgg catggctgga aacgctgaaa gctgaactgg gtattccgaa atctatccgt
     3661 gaagctggcg ttcaggaagc agacttcctg gcgaacgtgg ataaactgtc tgaagatgca
     3721 ttcgatgacc agtgcaccgg cgctaacccg cgttacccgc tgatctccga gctgaaacag
     3781 attctgctgg atacttacta cggtcgtgat tatgtagaag gcgaaactgc agcgaagaaa
     3841 gaagccgctc cggctaaagc tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg
     3901 tctggcaaca taaacggccc cttctgg
//
LOCUS       DAZGAP010000306         3910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGAP010000306 DAZGAP010000000
VERSION     DAZGAP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.308"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..1559
                     /gene="dtpD"
                     /locus_tag="ACHLAM_005050"
     CDS             78..1559
                     /gene="dtpD"
                     /locus_tag="ACHLAM_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO7856428.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(1602..3020)
                     /gene="phrB"
                     /locus_tag="ACHLAM_005051"
     CDS             complement(1602..3020)
                     /gene="phrB"
                     /locus_tag="ACHLAM_005051"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO7856429.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(3017..3526)
                     /gene="ybgA"
                     /locus_tag="ACHLAM_005052"
     CDS             complement(3017..3526)
                     /gene="ybgA"
                     /locus_tag="ACHLAM_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO7856430.1"
                     /translation="MNQQRFDESTLIRIFALYELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRGGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(3668..3910)
                     /locus_tag="ACHLAM_005053"
                     /pseudo
     CDS             complement(3668..3910)
                     /locus_tag="ACHLAM_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          877 a         1021 c          992 g         1020 t
ORIGIN      
        1 tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgcag
       61 tcaattgtgg aattattatg aataaacacg catcacaacc gcgcgctatt tactatgtcg
      121 ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg ctgatcctct
      181 atctcaccaa tcaactaaaa tatagcgata atcacgccta cgagttattt agcgcctact
      241 gttcgctggt atatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca
      301 atcgcatggc ggtgatgctg ggtgcgttgt tgatggcgat cggtcatgtg gtgctgggtg
      361 ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtt tgtggctatg
      421 gtctgtttaa atccaacgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc
      481 gtcgtgatgg cggtttctcg ctgatgtatg cggcgggtaa cgtggggtct attatcgcac
      541 ctatcgcctg tggctacgcc caggaagagt acagttgggc gatgggcttt ggcctggcgg
      601 cggttggcat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttctctcata
      661 cccgcggcgt taacaaaaaa gtactgcgtg cgacaaactt tctcctgccc aactggggat
      721 ggctgctggt tctgctggtg gcaacacctg cgctgatcac cgtactgttc tggaaagagt
      781 ggtcggtata cgccttgatt gtcgcgacta ttattggcct gggtgtattg gcaaaaattt
      841 atcgcaaagc agaaaaccag aaacagcgta aggagctggg gctgattgtt accctcacct
      901 tcttcagcat gttgttctgg gcattcgcac aacagggggg tagctcgatt agcctttata
      961 tcgaccgctt cgttaaccgc gatatatttg gttataccgt tccgactgct atgttccagt
     1021 cgattaatgc cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gttaaagaaa
     1081 gcgtcgcagg taatcgtacc gtgcgcatct gggggaaatt tgctcttggt ctcggcctga
     1141 tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtctgcaatg tatggtcact
     1201 cttctctgcc actgatggtg ttaggcctgg cggtgatggg gtttgcggaa ctgtttatcg
     1261 acccggttgc tatgtcgcag attacgcgca ttgaaatccc cggcgtgacc ggcgtattaa
     1321 ccggcattta tatgctgctt tccggcgcga ttgcgaacta tctggccggc gtgattgccg
     1381 atcagacatc gcaggcttcg tttgatgctt ccggagcgat caactactcc atcaatgcat
     1441 atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtgctga
     1501 tgatttggct gtatcaggcg cttaagttca gaaaccgcgc gctggcgctg gaatcttaac
     1561 aggccagcac cgatgcctga taagacgctc gctgcgtttt atcaggctcc tttcctcgct
     1621 tcttcgtacg ctgccagcgt tcgcagcctt gcctctttgt gatcaactat cggttgcgga
     1681 taatccagcg tcacacctgc tttctgcgcc cacttccacg gctcatgcac cactttccct
     1741 ggcacatcgc gcagttccgg tagccactgg cggataaact cgccctcacg gtcaaatttc
     1801 tcgccctggg ttgtcgggtt aaaaatacga aaatacggcg ctgcatcggt tccggttgaa
     1861 gcggcccact gccagccacc gttattggct gccaaatcac catcaatcag ctgcgacatg
     1921 aaatatcgct caccttcgcg ccagtcgatc aataaatctt tcaccaaaaa actggctgta
     1981 atcatccgta gccggttatg catccagccg gtgctgttaa gctgacgcat ggcagcatca
     2041 acaatcgggt atcccgtttt gccttcctgc caggccttta aatgcgcgga attgctctgc
     2101 cactgcacgc ggtcggtcca gacaataaac ggacgatgtt tacacagcga ggggtaatac
     2161 gttatcagat gacggtaaaa ctcgcgccag atcagctcat taagccagat actaccggcc
     2221 ccaccgtcca gcgcctgcgg ctgttccgcc aacaggcgat gcaaacactg gcgaggcgat
     2281 aacccgcccg ttgccaggct ggcggacaaa cggctggtgc cttctaccgc agggaaatct
     2341 cgctgttgct catactctcc ggcaccgttc tggcaaaact ggcgtaattg cgcaatcgcc
     2401 gctttctctt ccaccgggaa atgcgcagta tcgaaaggct gacgaggata attcagcgta
     2461 atggatggcg cgggatctat cgatccgcta ctacgtactt ttggcgcagc gacgcactcc
     2521 ggcatccctt ctcgcagccg tttaagccag gcgtttttaa atggcgtaaa gactttgtac
     2581 atctcatgat tgccagtcat caccgccccc ggcggcagga tcacgccgtc gtcaaatcct
     2641 tcacacacca cgttacgcag cgccctctca gcctgcacat cccgcgcccg ctcattcacc
     2701 tcatactgat agttataaaa cagatgggta acacgatttt ccgcgcacac ctgtttaact
     2761 atttcgacgc tggcggcaaa gtcatccact tcatgaaaca gcaaaggaat gcctttttcc
     2821 gccagcgcga tttgtagccc gttcagttgt gcattgataa attcagcctg acgcggcgac
     2881 atattatgcg ccgcccactg gcgtggtgta gcgatataca acgccagcac gcttgcagac
     2941 gaattgcggc aggcggcagc cagcgcgaga ttatcatgct gacgtaaatc ctggcgaaac
     3001 cagaccagat gggtagtcat aaaactcctg aatggcgcaa gttaatcaca cgcggccaaa
     3061 gttcgaaata acgctgccct gaaagccagg cgtcaggata aagcgccata tagtgtttga
     3121 tacgcatcag cggcgcaaga agcggttgct cgccaccgcg ataactgtcg ataagcgccg
     3181 ccagctgctg gcgttctgtg gaatcaatat gagggcgaaa ataaccctga acgtgcatca
     3241 aaacattggt gtggtcacgt cggttggcgg ggtgagaaag caaaacaact acgcgttggc
     3301 ggtactggtt gtaatagtca tccagatttt gccactggtg aatatcagcc acgaacgggc
     3361 caagtttgcg gtactcgggc tgggaatggg ccagaaagac gagtttatag cggctgtgat
     3421 aatcgttgag tgccccgcgc gttaagccat gttctttcag tcgatgtaac tcataaagcg
     3481 cgaagatacg gattagagtg ctttcatcaa atcgttgctg attcattact gctcctgaga
     3541 tattaacagg agtaagtgta gacgttggca aatgatgttt gtgaagttaa tcctgttgta
     3601 gcagggcaaa attacgaaag cacgctcccc gcaaggactg acgccagata ttttctgtcc
     3661 atggctgccc tttccattca aacgtgaaat caataagttc atcagggaca tcgcaaacaa
     3721 tatgaagacg gatttcttct ctgccgtgac tcttttcact cattgcgtaa ctgccatgct
     3781 ctggattatt taattctttc agcggaaatt tttcctcgaa ggctttattt agccgcccct
     3841 ggtttccttt tacagcgaat aaataatcac cgccctgttt ttgtatcttc tctgcaatat
     3901 ctttctggca
//
LOCUS       DAZGAP010000307         3903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGAP010000307 DAZGAP010000000
VERSION     DAZGAP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.309"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..2254
                     /locus_tag="ACHLAM_005054"
     CDS             113..2254
                     /locus_tag="ACHLAM_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO7856431.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2362..3693)
                     /locus_tag="ACHLAM_005055"
     CDS             complement(2362..3693)
                     /locus_tag="ACHLAM_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7856432.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWSTYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMRFYLINNEYYEQIKVCLQPKNI"
BASE COUNT         1179 a          903 c          823 g          998 t
ORIGIN      
        1 tgattctcaa ttacatatcc gatggcgaaa taacaaacgg atagtgctta gtgagcaata
       61 agaaacaatt ttcactcgcg acaatccgcg agtttttggg ataacagact aaatggctat
      121 gttcacacct tcattctcag gactcaaagg tcgggcgctc ttttcactgc tttttgcggc
      181 accgatgatt catgcaacag actctgtaac gaccaaagat ggcgaaacaa tcactgttac
      241 agcagatgca aataccgcaa ctgaggcgac cgatggttat caacctctga gcacttccac
      301 ggcgacatta accgatatgc cgatgctgga tatcccgcag gtggtcaata cggttagcga
      361 tcaggttctg gaaaaccaga atgcgacaac gctggatgag gcgctttata acgtcagtaa
      421 cgtggtacag accaatacat taggcgggac tcaggatgcc tttgtacgcc gtgggtttgg
      481 cgcaaaccgg gatggctcca tcatgaccaa cggtctgcga accgtacttc ctcgcagttt
      541 caacgccgcc acagaacgtg tggaagtgct aaaaggcccg gcctccacgc tgtatggcat
      601 tctcgatcct ggcggactga ttaacgtcgt gaccaagcgc ccggaaaaaa cattccatgg
      661 ttcggtttca gccacctcct ccagttttgg tggcggcact gggcaacttg atatcacagg
      721 tcccattgaa ggcactcagc tggcgtatcg ccttaccggg gaagtgcagg atgaagatta
      781 ctggcgaaac ttcggtaaag agcgcagtac atttattgcc ccgtcactca cctggtttgg
      841 tgataatgca acagtaacca tgctctattc ccatcgggac tataaaactc cgttcgatcg
      901 tggaacgatt ttcgacctta cgacgaaaca gcccgtaaac gttgatcgaa aaatacgttt
      961 tgacgaaccg tttaatatta cagatggtca gtccgatctg gcgcaactca acgcagaata
     1021 tcatctcaat agccagtgga cagcgcgctt tgattacagc tacagccagg ataaatacag
     1081 cgataatcag gcgcgtgtta ccgcgtatga tgcaacgaca ggaacactga cacggcgtgt
     1141 tgatgcaact cagggatcta cccagcgtat gcatgctact cgtgcggatc tgcaagggaa
     1201 tgttgatatt gccggattct ataatgagat tctgggtggg gtgtcatatg aatattatga
     1261 tcttctgcgt acagatatga ttcgctgtaa aaaagctaaa gatttcaata tctacaaccc
     1321 tgtttatggt aataccagca aatgtacaac ggtttcggcg tcggacagtg atcagacgat
     1381 caaacaggag agctactcag cgtatgcaca ggacgcactc tatctgaccg ataactggat
     1441 tgccgtcgcc gggatccgct atcagtatta cacgcagtat gcgggtaaag gccgtccttt
     1501 taatgtcaat actgacagcc gcgatgaaca atggactccc aaactggggt tggtctacaa
     1561 actgacgcca tcggtatcct tatttgccaa ttattcgcaa acatttatgc cgcagtcgtc
     1621 aattgccagc tacattggcg atcttccacc ggaatcatct aatgcttacg aagtcggggc
     1681 aaaattcgag ctattcgatg gtatcaccgc agatattgcg ctgtttgata tccataaacg
     1741 taatgtgttg tataccgaaa gtgttggtga tgaaaccatt gccaaaacgg caggccgtgt
     1801 tcgttcaaga ggggtagaag tcgaccttgc gggagcatta actgaaaaca ttaatatcat
     1861 tgccagctac ggctataccg atgctaaggt tctggaagat cctgattatg caggtaaacc
     1921 attgccgaat gttcctcgtc ataccggttc gctattcctg acctatgaca ttcataacat
     1981 gcctggcaat aacacactga cgtttggcgg tggtggacat ggcgtaagcc gtcgttcggc
     2041 aaccaatggg gctgactatt atctgccagg ctattttgtt gccgatgcct tcgccgcata
     2101 caaaatgaaa ttgcagtatc cggtcacact gcaattaaac gtcaaaaacc tgtttgataa
     2161 aacgtattac acctcttcca tcgccacaaa taatctgggg aatcagattg gcgatccgcg
     2221 tgaagtgcaa ttcacggtga aaatggaatt ttgatcaaaa aagcccggta tgtttaccgg
     2281 gctgagactg atgacaaact ccaaattgtc tgatgcgcta cgcccatcag atctaccata
     2341 gaaattcaat atattaaatt ttcatatatt tttaggctgt aaacacacct taatctgttc
     2401 gtaatattca ttattaatca gataaaatct catgatgagt ccggtaatca ggttaagtat
     2461 cgcgggaatg atcgtcatta acaggcagat cccccacaac gcctgtggtg tttgttctac
     2521 gccaggttga tagtccataa acgctaaagc gattccgata aaccctcccg ccagtcccat
     2581 accaaacttt tgaaaaaaga gaatcccgcc aaatgccagt ccagaaacac gttgccccgt
     2641 tttcatttca ccataatcca ctgactcagc aatggatgcc caataaatgg gaagctgaat
     2701 atcagtcaga aaggtcacca caatataaaa tgtaaaagct aacactatag aatttggttg
     2761 cacgaaatac atcgcaccac ccaccacgaa cgccagtaat tgggtataac gaaatatttt
     2821 tattttgtcg tagagtcgag taagccaggt gcaagcgatc atagccagaa cagaggcgat
     2881 cacgcctgaa gttaaaaacg gtgaaattaa agcatcccca ccattcaggt aatattttgc
     2941 ataatatgca gcgacagagt tacgaataat cccgccaaac ataatgatcg caattacagc
     3001 gcccaaaatg agccattgat catttttgat cagtaaccga aactgcgcgg ccataccgag
     3061 actgggtttt ggatggtaga tgcgctctct cacgctaaaa aaacaataca ggcataaaca
     3121 cgttgccaga attcccattg cccccattgc tatttgataa cccagttgta cgttattctt
     3181 cccaagatac attgcagata aaggaacaat aattgttacg gcgaacatgg caatttttgt
     3241 cataacaaaa cgataaccat tggcgctcaa tcgctcttgt ggatcatccg tgataacgct
     3301 gattaatgaa acataaggaa tagcgattaa cgtataggtc aaggtaagta acacataggt
     3361 agaccaggcc cataaaagct taactgaata atcggtatta ggtgtggtga acatcaacca
     3421 aatgcttatc ccaaacggta ttgacataaa aagcaaccac ggccgatatt ttccccaacg
     3481 tgtattcgtt ctgtctgtca ttgttcccac aactggatca atcacagcat caaggatacg
     3541 tacagaaaat aacagaatac ctaagtcgac gggtttcaga ccaaatacat ccgtgtaaaa
     3601 ataagtgatg atcatggcca aagacatcat tactatcgcg atagccatat caccagcacc
     3661 aaaacctatc ttttcattta ttgtaagttt catacacagc actcattgat tacgtgaact
     3721 tatattaaaa acaaatatat ctgaactcat tcagcacata actttacgag tgagcataaa
     3781 tgcattggtt gataccacaa caaaccattg agtcaatatt gagcaacatc acataaaaac
     3841 caatttcact taatctcatg atttatatat ttaattgtgg ttttttctgt tttatgtcac
     3901 aga
//
LOCUS       DAZGAP010000308         3859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGAP010000308 DAZGAP010000000
VERSION     DAZGAP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.310"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..193
                     /locus_tag="ACHLAM_005056"
     CDS             <1..193
                     /locus_tag="ACHLAM_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856433.1"
                     /translation="SIEKEQLIRDEINKEHLTAHQNRINVAEVADSDKSEFSRIKTSS
                     DFDGALWTGIRKLISFFKK"
     gene            complement(1185..2495)
                     /locus_tag="ACHLAM_005057"
     CDS             complement(1185..2495)
                     /locus_tag="ACHLAM_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFO7856434.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
     gene            complement(2508..3779)
                     /gene="dcuC"
                     /locus_tag="ACHLAM_005058"
     CDS             complement(2508..3779)
                     /gene="dcuC"
                     /locus_tag="ACHLAM_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7856435.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
BASE COUNT         1171 a          816 c          762 g         1110 t
ORIGIN      
        1 cagcatagaa aaagaacagc taattagaga tgaaattaac aaagaacatt tgacagctca
       61 tcaaaataga ataaatgttg ctgaggttgc tgattctgac aagagcgaat ttagtagaat
      121 taaaacctct tcggattttg atggggcatt atggactggg ataagaaagc ttatttcatt
      181 ctttaaaaaa tagatctata gcgtatcgtt ccggcgaact catcctgatg cgttttcggg
      241 gggccatcgg tgaaacatac gaatgatcgc cctctgacga tggatgattt attttgttgc
      301 acagaagcga tcgctgggca ggtaccggag gttggctctg aagctctaat tgcgtcaggt
      361 tgcgaagtgt tgacacgggt attggtgaga actgccccgg gaacgactct gggctgattc
      421 tggttctgat tcgctgtcag cccgtgtggt tactggcctg gctgatttcc tgtcgtcagg
      481 cgtttaactc ccgactgcag gtatcactta ccaagagaag gtaaatcctt ttagacgtac
      541 agtgaaagtt gtctggtcat aatctgaagc atctgacaaa gttgtgtcca ccaaataata
      601 agtagtggga accaaagtgt catatataca gaaaaatata actcaccgat ttccttcacg
      661 ttaatacgca gggggaggta tataacagtc cgttcaggta ttggataaat atttagagtg
      721 gaatgattac agttatactg tggatgggag aagatattta ctgttaattg attatcattt
      781 ttaatttgta ttataataac aaggtcccgt aaattccgga ctacagagtc cagccagttt
      841 gctcatccgt caattcaggt agatgcgtca ggctttgtgg ctggcggaaa atggctcgtt
      901 cgctgatatt gttgcgtcat acaaaaacag tacgtgatga gtatgaggct tttttatctg
      961 tatataatct cttattctgt aaattaggat agggtaatct cgcaatcatc ctcttcccat
     1021 atcaccagta tatgctgagc cgtgtttgtc tatttggtta aaattcgcta caatcctatt
     1081 ctctcttttg ggaatataaa agtctccatt ttctctttat tcgttaatgg agactgtatt
     1141 taaaggcaga aatcaggagt gattgacagg atacctgtca gcaatcaata caattcagat
     1201 tccagttttc cgttccctac aattttattt agtccgggag agttcaatct ctccagacat
     1261 aacgaaaaca gtttaacgcc tgtatccagc acattctcat caaaatcaaa atcaacttcg
     1321 tgatgaccag cttttaaatc actgccaagt attatatatg ctgcttttcc tcctatcttc
     1381 tgtacacggc gaataaggag cgttgcatct tcactgcccc caaatgattt ctctgtcaaa
     1441 gacatgttca tatcttcttg tgctatatag tgtatcagat tgacaagtaa agggtcatta
     1501 tgtaaatccg ttgcttcacc aaccactttg tactcaaaac gtaaaccaaa tatggaagaa
     1561 ataccttgtg ctattttcag agcatgttca cgcatgtatt gatttattgt ttccgtttct
     1621 ccacgaactt caagttgcat ccttgcaaaa gacgggatta tgttccttcc ttcacctgcg
     1681 tttagcgtgc caatatttat ccgggacata ccttcaccat ggcgtggaat ggcaagtaat
     1741 tgcgttgcac agtgacaagc agctgccaga gcattatttc ccaggtgagg atttacacca
     1801 gcatgtgccg gagtaccata gaaaaagaaa tccatttttg tcgtacacaa caaatctgta
     1861 ggtgaagcga caacatttcc tgtaggaact cccataccaa tatgtgaaga aaggaagtag
     1921 tcgcagtcat caagaatacc accttctgca ataggttttg caccgcgaac gccttcttct
     1981 gcagcctgaa aaattagctt aattttcccg cataacttat ggttattttc agatacccat
     2041 tgtgccaatc ctaaaccaat cgatatatga ccatcatgtc cacatgcatg cattaagcca
     2101 gagtgtatgg atgcaaatcc cattttttca ggaatatgat ctttcgattt tgattcactt
     2161 acctgtacac aatcaatgtc aaaacgaaac gcagtgacag ctccattttt tcctgtatcc
     2221 cataaagcaa cacaccctgt aaagccatcc atttcatcca gcaattcatg gggaacgcct
     2281 gaatcaactg cacgttttat actatctgca acttcagttg cattacggcc cataatatac
     2341 tctgttgaca ggtatttatt ccccgtaata acctgccatc ccataccacg cagaatagta
     2401 attatctttg acgttgtgat aaattcgcac cacccacttt ccggaatatg gtgaaattct
     2461 ctccgccatt tttgtaactg attcagttca gacatatacg cctcctgtta aaggaataat
     2521 aatgcaataa acaataccgt cacaatcatt ccgggggcag tgcgttttgc aatttcaact
     2581 ggattaactc cagccagacc ggcacagata atagttgcac cagccaacgg agacatgttt
     2641 ctccctagcg cagaagccag cataactgca gcacccagag tatttgtatc gtaaccaaaa
     2701 ttgttcgcat gtggtgtgac aacttcgttg aatgcgaatg cagtcgcatc ccctgtgccg
     2761 gttataagtc ccatcaggaa tggccccaat gtaccgcccc agcgggcaaa ttctggtgag
     2821 tcagtaagaa atctgataaa cacatcaatg ataccaacag atcttaaacc ttcaatgaaa
     2881 acagctgcgg aaattatgat cccaagaata tttgcatatg atgtccccat accctcaaag
     2941 aagctggaaa ctaattcttt gggattggat ttactgacaa tcagggcata aagacaaccg
     3001 agaatcattg cggctggaac ggttatttta gtctctccaa gaaaaccaaa ttcagccagc
     3061 ataagtaatg ctacgggaag aaatggtgct acagatttca gataaaaaca gatatcacgg
     3121 tcagtgaact caacctgtgt ttgttctgat atgtttcctg cagaatcaca ttttgtttct
     3181 tttcttacaa tagcaacaac tgtaagaata acagcacata ttacaccaca tgttattgta
     3241 gtcggagtat ggaatacgac gaattccatt aatggtgttt gtgatatttt agatataaat
     3301 gaattatgtg caacaccggg attgaggtaa gcaccgatag ttcctgaaag gatagctgct
     3361 ccggctattg caggatgaat acgtgctgaa ataagtaatg gtatcagcgt tgcaccaaca
     3421 gcagctgatg ctccagcagc agatggaatc gcgatgcaaa caataaaact gacggtaacc
     3481 gcagcaggaa taagaaagat accaagacga gataatcctt tcgttagaaa atgaaccagg
     3541 catgtatcac aaccagtgaa tttcataaca caggcaaagc ccatactggt acaaatggcc
     3601 tgaatgagac cggactcagt cattctgctt gcaaatgccg aaaatgcgtt taaaggtgac
     3661 atggcaacta tacatagcag aatacctgca ccaattagtg tcgttcgtgt gtcgtatctt
     3721 tttatcaaaa gcacaactgt gattatcaga atcacacatc cagttatcac gttaaacatg
     3781 agatcctcct tcttcaaaga aggataatct ttactttatg tgatttgctc aaatattgat
     3841 catgaacaaa aaaatcaat
//
LOCUS       DAZGAP010000309         3828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGAP010000309 DAZGAP010000000
VERSION     DAZGAP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.311"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..217
                     /locus_tag="ACHLAM_005059"
     CDS             <1..217
                     /locus_tag="ACHLAM_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856436.1"
                     /translation="PHNNNERSGYIKASNIASEKNMVNITSVADDINKIISSVSGFDS
                     GDERLASLYNLSLHQNNTHSTTLTTTV"
     gene            267..2387
                     /gene="ehxB"
                     /locus_tag="ACHLAM_005060"
                     /gene_synonym="hlyB"
     CDS             267..2387
                     /gene="ehxB"
                     /locus_tag="ACHLAM_005060"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO7856437.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNSENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            2391..>3828
                     /locus_tag="ACHLAM_005061"
     CDS             2391..>3828
                     /locus_tag="ACHLAM_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO7856438.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGAFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNARGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESFRER"
BASE COUNT         1236 a          608 c          803 g         1181 t
ORIGIN      
        1 acctcataat aataatgaac ggtcaggtta tataaaagcc agtaatatag catctgaaaa
       61 aaacatggtt aatatcacca gtgttgccga tgatattaac aagattattt cttcagtttc
      121 agggttcgat tcaggtgatg aacgattagc atctttatat aatttatcct tacatcaaaa
      181 caacacacat tcaacaactt taacgacaac tgtctgagac taaaagcgga ggtgatggcc
      241 attccaggag gaatggtcat cgttatataa tgagtaaatg tagttctcat aatagtctgt
      301 atgcactgat attgcttgca caatatcata atataactgt caatgctgaa actataaggc
      361 atcagtataa tacccacaca caagattttg gggtgactga atggttactg gcagcgaaat
      421 ccattggctt aaaagcaaaa tatgtagaaa aacatttttc cagattgtca ataatttctt
      481 tacctgcgtt gatatggcgg gatgacggta agcattatat attatctcgt gttactaaag
      541 attcatcacg ctatcttgtt tatgatccag aacagcatca gtcactaact tttagtcggg
      601 atgagttcga aaaactgtat cagggaaaag tcattctggt tacgtcaaga gcaacagtag
      661 tcggagagtt agctaaattt gatttttcct ggtttatccc ctctgttgtg aaatacagga
      721 ggattttact tgaggtgtta actgtttctg cttttattca gtttcttgcg ttaataacac
      781 ctcttttttt tcaggttgta atggataagg ttttagttca ccgggggttt tcaacgttaa
      841 atattatcac aatagcattt attatagtga tactttttga agtgatatta accggagcca
      901 gaacttatat tttctctcat actacaagtc gtattgacgt cgaactgggt gctaagttat
      961 tcagacattt gcttgcattg cctgtttcat attttgaaaa tcgcagggtc ggagagaccg
     1021 ttgccagagt aagggaactg gagcaaattc gtaatttttt aaccggacaa gcgttgacat
     1081 cagttcttga tctgtttttt tctgtaatat ttttttgtgt catgtggtat tacagtcctc
     1141 aattaacact ggttatatta ttgtcactac cttgttatgt tatatggtca ttgtttatat
     1201 cacccttatt acgtcgacgt cttgatgata agtttctcag gaattcagaa aatcaagctt
     1261 ttcttgtcga aacggtaaca gcaataaata caattaaatc catggcagta tcgccacaga
     1321 tgattgctac atgggataaa caactggccg ggtatgttgc ttccagtttc agggtaaatc
     1381 tggttgcaat gacagggcag caggggatac agctgataca gaaaagtgta atggtaatta
     1441 gtctatggat gggagcacat cttgtcatat cgggagagat aagcatcggg cagttaatcg
     1501 cgtttaatat gcttgcaggt caggtcattg ctcctgttat cagactggct catctttggc
     1561 aggattttca gcaagttggt atttctgttg agcgccttgg ggatgtatta aatacaccgg
     1621 tagagaaaaa gtcaggcaga aatatactgc cggaaattca gggggatatc gaatttaaaa
     1681 atgtcagatt tcggtattct tctgacggta atgttatttt gaataatatt aatttataca
     1741 tatcaaaggg ggatgttatc ggtatagttg gccgttctgg ttcaggaaaa agtacattaa
     1801 caaaactgct tcagcgcttt tatataccag agaccggaca gattttaatt gatgggcatg
     1861 atttatcact tgcagatcca gaatggttac gacgccagat tggtgttgta ttacaggaaa
     1921 atatactatt aaatcgtagt attatcgata atattacatt agcttctccg gctgtatcta
     1981 tggaacaggc tattgaggca gccagacttg caggtgccca tgattttatt acagaactaa
     2041 aagaagggta caatactatt gttggagaac agggcgttgg tctttcagga gggcagcgcc
     2101 aacggatcgc aatagcccgg gctcttgtta caaatcctcg aattcttatt tttgatgaag
     2161 caaccagtgc tcttgattat gagtcggaaa atataataat gaaaaatatg tcaagaatat
     2221 gtaagaacag aaccgtaatt attattgcac acaggttgtc aactgtaaaa aatgcaaaca
     2281 gaataattgt tatggataat ggatttattt ctgaagatgg cacacataaa gagcttatct
     2341 ccaaaaaaga cagtttatat gcatatttat atcagttgca ggcataacgt atgcgctttt
     2401 acatgaaagg tttatgggat ttgatttgcc gttataagac ggttttttct gatgtctgga
     2461 aaattcgtca cacacttgat gctcctgtga gagaaaaaga tgagtatgca tttcttcctg
     2521 ctcaccttga actcattgaa acaccagtat ccagacgttc tcattttgtt atatggagta
     2581 ttttattatt tgtaattata tctcttctct tatctgttct tgggaaagtt gaagtggtct
     2641 cagtagcaaa tggtaagttt actcatagtg gaagaagcaa agaaataaaa ccgatagaga
     2701 atgcgattgt agaaaaaata atggtgaaag atggcgcttt tgtaaaaaaa aatgatccat
     2761 tagttgaatt gacggtgcct ggtgttgaat ctgatatttt aaaatcagaa gcatctttgt
     2821 tgtatgaaaa aacagaacaa tatcgctacg caatcctttc tgaatcaata caacggaatg
     2881 agcttcctga aataaggata acagattttc ctggcggaga agataatgca agaggtgaac
     2941 attttcagag ggttagctca ttaataaaag agcaattcat gacatggcag aacagaaaaa
     3001 atcaaaagca gctcacatta aataaaaaaa tagttgaacg ggatgcagcg cttgctcgtg
     3061 ttagcctgta tgagcatcag gtatcacagg aaggaagaaa acttaatgat tttaagtatt
     3121 tgttgaataa aaaagctgtt tctcaacatt cagttatgga gcaggaaaat agctatattc
     3181 aggcaaaaaa tgaacatgca gtctggcttg cacaggtttc tcaacttgaa aaagaaatag
     3241 aacttgtgcg ggaagaactg gcactggaga cgaatatctt cagaagtgaa attatcgaga
     3301 agcacagaaa atcaacagat aacattgtgt tactggagca tgagcttgaa aaaaacaggc
     3361 agagaaaagc atcatctttt attaaagctc ctgtgagtgg tactgttcag gagttaaata
     3421 tacatacaga aggtggtgtt gtcacaacag cagaaacgct gatgattatt gtccctgata
     3481 atgatattct cgaagtaaca gcatctgtac ttaacaagga tatcggtttt atacaacctg
     3541 gacaggaggt cgttattaaa gtagatgcat atccatatac acgtcatggt taccttacag
     3601 ggaaagtaaa aaatataact gcagattctg tttctgttcc ggatacagga cttgtattta
     3661 acgtgattat atctgttgat cggaatgata tacagggaga aagaaaaaaa attcctgtta
     3721 cagctggaat gactgttatg gcagaaataa aaacaggagt gcgtagtgtt atcagctatc
     3781 ttcttagccc attaaaggaa actattaatg aaagtttccg tgaacgtt
//
LOCUS       DAZGAP010000310         3801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGAP010000310 DAZGAP010000000
VERSION     DAZGAP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.312"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1638
                     /gene="dpiB"
                     /locus_tag="ACHLAM_005062"
     CDS             <1..1638
                     /gene="dpiB"
                     /locus_tag="ACHLAM_005062"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO7856439.1"
                     /translation="KQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTASFEDYLTL
                     HVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGDRHSIRLY
                     HPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVIGVVSIGY
                     LVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIARVVRQQE
                     ALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPADFFTEQI
                     DEKRQDVVANFNGLSVIANREAIHSGDDLLGAIISFRSKDEISTLNAQLTQIKQYVES
                     LRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFADRQVAGLL
                     FGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLRSDEGNKI
                     VELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLYLIASYVT
                     RCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1607..2287
                     /gene="dpiA"
                     /locus_tag="ACHLAM_005063"
                     /gene_synonym="citB"
     CDS             1607..2287
                     /gene="dpiA"
                     /locus_tag="ACHLAM_005063"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO7856440.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2328..3713)
                     /gene="dcuC"
                     /locus_tag="ACHLAM_005064"
     CDS             complement(2328..3713)
                     /gene="dcuC"
                     /locus_tag="ACHLAM_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7856441.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1018 a          915 c          992 g          876 t
ORIGIN      
        1 aaacagtttg catttttcca aagactggca tttccgctgc gtatcttttt gctgattctg
       61 gtgttctcaa tatttgtcat tgcagccctg gcgcaatatt ttacggccag ttttgaggac
      121 tatttaacgc ttcatgtgcg cgatatggca atgaatcagg caaaaattat tgcttctaat
      181 gacagtatca tcagcgcagt gaaaacgcgt gactacaaga ggctggcgac catcgctgat
      241 aaattacaaa gagatactga tttcgattat gtggtgattg gcgatcggca ctcgattcgt
      301 ctttaccatc ctaatccgga gaaaattggt tatcctatgc agttcaccaa accgggcgcg
      361 ctggagaaag gggagagcta cttcatcacc gggaaagggt caattggcat ggcgatgcgt
      421 gccaaaacgc caatctttga tgacgatgga aaagtcatcg gcgtggtgtc gattggctac
      481 ctggtgagta aaatcgatag ctggcgggct gagtttttat taccgatggc tggcgtgttt
      541 gtcgtgctgt tagggattct gatgttgctg tcgtggttcc tggccgcgca tatccgtcgg
      601 cagatgatgg gcatggagcc aaagcaaatc gcacgcgtgg tccgtcagca agaggcgctg
      661 tttagttcgg tttatgaagg gctcattgcg gtggatccgc acggttacat taccgccatc
      721 aatcgtaacg caagaaagat gctggggctg agctcccccg gacggcaatg gttgggtaaa
      781 cccattgctg aagtggtcag gcccgccgat ttctttaccg aacagattga tgaaaaacgt
      841 caggatgtgg tggcgaactt taacggtctg agcgttattg ccaaccggga agctattcat
      901 tctggtgatg atttgctggg ggccattatc agctttcgta gtaaagacga aatatccacc
      961 ctcaatgcac aactgacgca aattaaacaa tacgtcgaga gcctgcgcac attgcgacac
     1021 gagcatctca attggatgtc gacgctcaat ggtctgttgc agatgaaaga gtatgatcgc
     1081 gtgttggcga tggtgcaggg ggagtctcag gcccagcaac agcttattga tagcctgcgc
     1141 gaggcgtttg ccgatcgcca ggtggcgggg ctgctttttg gtaaagtgca gcgcgcccgc
     1201 gaactggggc taaaaatggt catcgtcccc ggaagtcagc tttcgcaact gccgccagga
     1261 ctggacagca ccgagtttgc agccattgtt ggcaatttac ttgataacgc cttcgaagcc
     1321 agcctgcgta gcgatgaagg aaacaagatc gttgaattat tcctcagcga tgaaggcgat
     1381 gatgtggtga ttgaagtcgc cgatcagggc tgcggcgttc cagagtctct acgagacaaa
     1441 atatttgagc agggtgtcag tacgcgtgct gacgagcccg gcgaacatgg cattgggttg
     1501 tacttgattg ccagctacgt aacgcgctgc ggtggtgtta tcactctcga agataatgat
     1561 ccctgcggta ccttattttc aatctatatt ccgaaagtga aacctaatga cagctccatt
     1621 aaccctattg atcgttgagg acgaaacgcc gctggcagag atgcatgcgg aatatattcg
     1681 tcacattccc ggattcagtc agatattact ggcgggaaac ctggcgcagg cccgaatgat
     1741 gatcgagcgt tttaagccgg ggctaatctt gctcgataac tatcttcctg acggtagagg
     1801 gattaattta ctgcatgaac tggtgcaggc gcattatccc ggcgacgtgg tgtttaccac
     1861 tgcagccagt gatatggaaa cggtgtctga agccgtacgt tgtggtgtat ttgattatct
     1921 cattaagcca attgcctatg aacggctggg gcaaacgcta acccgtttcc gccagcgtaa
     1981 acatatgctg gaaagtattg atagcgccag ccagaagcaa atcgatgaga tgtttaatgc
     2041 ctatgctcgc ggtgaaccca aagacgaact gccgaccggc attgacccct tgacgctaaa
     2101 cgcggtgcga aaactgttta aagagcctgg tgtgcaacat acggcagaaa cggtggcgca
     2161 ggcactgact atcagccgca ccactgccag acgttatctt gaatattgcg ccagccgcca
     2221 tctgattatt gctgaaattg ttcacggcaa agttggcaga ccacaacgca tataccacag
     2281 tgggtgacat gataaagccg gaggaaactt gcctccggca tgactattta cttgcctgtg
     2341 accgcggctg ccgttcctgg caccatcagc tctgtagcaa cgataacaat caccagccca
     2401 acaagcaccg gcaccgaggt gcgttttacg acttcaaatg gtgagatttt cgccatcccg
     2461 gcaaccgcaa cgactacgcc agaaacgggt gacagggtac ggccgaggtt tgacgcctgc
     2521 agcattggga tagtcaaata cgccgggtta atgccggaag agtgcgccag tttcgggatc
     2581 atctcaacaa acgcataaaa cggcgcattg cctgaaccgg tcgtgactgc cgccagcatt
     2641 gtcaggatca ccaataccag catcaggatg atgctcgccg aaccaaacga ggtagcgata
     2701 gaaatcagac tttgaataaa gccgatggtg ctaagcccct gagcgaatac cccagcggca
     2761 accagcagca tcaccacgtt tgcaaatgca tctgccatcc cgcgataagc cacttccaga
     2821 ccagagaaaa ctttctgggt attaaagctg cggatgaact ccagaatgga ggcaatcagc
     2881 atacaaatca ccagaatagt gatgatgtgt aattgcggac cccatttgcc gtcaaaaatc
     2941 agcactccga tgatcggcgt gaacggcaaa atggcataaa acgcaggggc agtggtggtg
     3001 atttcactga catctaacat ttcatgagag atgtgttctt ttttatccag ataacgttgc
     3061 cagaagaagt gggcgattgc catgccgata attgcagcaa ttgagatagg cagcgttgtt
     3121 ttgaaggcga agtcaatcag cgacatttcg gaagcctgcg ccgccagcac cacatcccct
     3181 gaagtcggtg cgagaataat cgccgccggg gaggcacaaa tagcagcagc tgcaccacgg
     3241 ctgataccaa cgtttaccat caccggaaac agggttgcca tcagcaaaac acccagaccg
     3301 gtcgcggaag agacggctag agacatcaga caggcgacaa aataggcggc aatcatcagc
     3361 aggtaagggg agttaatata ctgcaatggt tttgacgcca acttgaccac catatcattc
     3421 gcgccgatat gggtcatgta agcggcaaag ccacacagca tcataatcat catgccgagg
     3481 tcgccgccgc ggctcattag caatatttta acgtattcaa cgatatccgt ggcgctgtag
     3541 cctgttgaag cctggctgga cggtaacact ttgtgcccca taatggcact gataatcagc
     3601 aataacaggc caccgacaaa taacacgcca gtggcggaat accctttaat gatgtagcga
     3661 gctacaccca caataaccac aaccccaata aggagttcaa tgaatgtcag cataattttt
     3721 cctgtctcca ggccccaaag taaataataa aaaattccta aagcttaagg aaaaaatatg
     3781 cccaataaat tggcgatgaa t
//
LOCUS       DAZGAP010000311         3712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGAP010000311 DAZGAP010000000
VERSION     DAZGAP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.313"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..137)
                     /locus_tag="ACHLAM_005065"
                     /pseudo
     CDS             complement(1..137)
                     /locus_tag="ACHLAM_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            445..558
                     /locus_tag="ACHLAM_005066"
     CDS             445..558
                     /locus_tag="ACHLAM_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO7856442.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
     gene            complement(802..1506)
                     /locus_tag="ACHLAM_005067"
     CDS             complement(802..1506)
                     /locus_tag="ACHLAM_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HFO7856443.1"
                     /translation="MRRLPLMVGVIVGVMLAVGCHDRRAGVKTLLGDGMDQWGQKLPY
                     DHWQFNFFYPKNLPALVTMVYLEDGNVRESIFRQLDAARPSQSSVDTWSQRVGSFSAS
                     FNIGKALPVRMTVCWDSVIDKKAYETEIWFSRDTWQQMLTAYPDTYKPGKTYYRDNMI
                     IGLAPGGAVRIWLEDNGEPVVLQHPARQFTLTGDDMLICKNVPNQIDFSYIKANGYDP
                     FIRDFIKEKPYPYGHW"
     gene            complement(1517..3208)
                     /locus_tag="ACHLAM_005068"
     CDS             complement(1517..3208)
                     /locus_tag="ACHLAM_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFO7856444.1"
                     /translation="MVEEDEKNVPGDTCYYRYEIIGDSTHRTSIHNSFKTIQCRRVPV
                     RGITLTVGVFFDGTGNNRANADDLRLAYAHCANLVDEERARACEKYEKRARAGLGNAS
                     WQGGHTNISRLFDLYQQSDELNDSETEAQVKAYISGIGTADGESDSILGKALGSSLIR
                     QFEGVVTKTDEALEKISAELTRFIKLNRDKVAIAKVQFDVFGFSRGAAAARHFANRVM
                     NQDAAIAAAIDKGTDLTGHHGKLAGEVRFLGLFDTVAAIGSLLNFYDVNGRSNPGVNL
                     ELRPSVAQKVFQISAMHECRYNFSLNSIAGMWPELALPGAHSDIGGGYNAGEDEISLL
                     TMPDFEVTRDSADEMQTEVYRKAEKMRQVLYSLPALKYLMPHGEVETKMISWPLVNQN
                     KARASIFEKKVGAAVFMTRRDVPNDWEKVSLRVMLDAAQDAGAVFTPILSSNKELALP
                     SELLPLCDKAIAQGRAVRRGAEPEGFTAEDMRTIGRYLHCSANWNIDSDLSLWVSPSS
                     GEVFLRNNYAPTDERSFVWPMAPGKGWVRTVWRMDDQQQWEERARVNADALDGLF"
BASE COUNT          849 a         1137 c          826 g          900 t
ORIGIN      
        1 gagtgacgcg acagcgtgct catctgcccc tggagccact catacaacga ctgcattagc
       61 ggtaccgttc tggcttttct gaccgccagt cgctcttctg ccggactgcc gcggatctca
      121 gactcgatag cgtacagtgc ctcgataggg ctgcgtttgg gaaacgtaca atattgtacg
      181 gttgcgctat tgcggtaggg tgtactccaa cgctatacgg tatttggcag acctttaggg
      241 acatgagata acctaaatgc aataagagaa aagcaataac ttaaaggcga tagctgatca
      301 cgatgaatca atcagttact tatccgcatc gattaaaaaa tgctcctaac gtacaataat
      361 tccgtttccc aaaccgaccc ctctcatagc tcacgctgtt ggtatctcag ttcggtgtag
      421 gtcgttcgct ccaagctggg ctgtatgcac gaaccccccg ttcagcccga ccgctgcgcc
      481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttaac gccactggca
      541 gcagccattg gtaactaaac aaggtggatt ccgatattag agtcttgaag tgatcaccca
      601 actacggcta cactgaaagg acagtattcg gtatctgcgc tccaccaagc cagttaccac
      661 agtcgattag aagaggtcga agacttctaa tcggacggaa aaagccgccg caaggcggct
      721 ttttccgttt acaagccgaa gattacgcgc agcttaaaag gatctcaaga agatcatttt
      781 aattttgctg atcgttgctc cctaccagtg accatacgga tagggctttt ccttgataaa
      841 atcgcggata aatggatcgt aaccattagc cttgatgtaa ctgaaatcaa tctggttggg
      901 aacgtttttg catatcagca tatcatctcc cgtcagagtg aactgccggg caggatgttg
      961 gagtacaaca ggctcgccat tatcctccag ccagatccgc acggcccctc ccggtgccaa
     1021 tccgataatc atattgtccc gataatacgt ttttcccggc ttataggtat caggataggc
     1081 agtcaacatc tgctgccacg tatcccggct gaaccagatc tccgtctcat atgccttttt
     1141 atcgataacc gaatcccaac acaccgtcat cctgaccggc agtgccttgc caatgttaaa
     1201 actggctgaa aaactgccta cgcgctggct ccatgtatcc acactactct gacttggtct
     1261 ggctgcatca agctgccgaa atatgctttc ccgtacatta ccgtcctcca gatacaccat
     1321 tgtcaccagt gctggcaggt ttttcgggta aaaaaaatta aactgccagt ggtcataagg
     1381 cagcttctgc ccccattgat ccataccgtc gcccaacagc gtcttcacgc cagccctacg
     1441 gtcatggcag ccgacagcca gcattacgcc cactatcacg cccaccatca gcggtaatcg
     1501 tctcattcct gcctcctcag aacagtccgt ccagcgcatc cgcgtttacc cgcgcccgct
     1561 cttcccactg ctgctggtcg tccatccgcc acaccgtccg cacccagcct ttgcccggtg
     1621 ccatcggcca cacgaacgag cgttcgtcag tcggagcata gttatttcgc agaaaaactt
     1681 ctcccgagga cggactcacc cacaggctca agtctgagtc gatgttccag ttcgccgagc
     1741 aatgcaggta acgcccgatg gtccgcatat cctccgccgt gaagccctca ggctccgcgc
     1801 cacgccggac agcacgcccc tgagctatgg ctttgtcaca caggggaagc agttctgacg
     1861 gcaacgccag ctctttgtta ctgctgagta tcggagtaaa cacagccccg gcgtcttgtg
     1921 ccgcatccag catcacccgc aaactcacct tctcccagtc gttcggcaca tccctgcgcg
     1981 tcataaacac tgccgcgccc actttcttct caaaaataga cgcacgcgcc ttgttctgat
     2041 tgaccagtgg ccacgaaatc attttcgtct ccacctcacc gtgcggcatc aggtacttca
     2101 gcgccggcag tgagtacagc acctgacgca tcttctccgc cttacggtac acctccgtct
     2161 gcatttcatc cgcgctgtcg cgcgtcacct caaaatccgg catcgtcagc agggagattt
     2221 catcctctcc tgcgttatag cccccgccaa tgtccgagtg cgcccccggc agcgccagct
     2281 ccggccacat ccccgcgatg ctgttcaggc tgaagttata ccgacactca tgcatcgcgc
     2341 taatctgaaa caccttctgc gccactgaag gacgcagttc gagattcacc ccgggattgc
     2401 tgcgtccgtt aacgtcgtag aagttcaaca ggctcccgat agccgccact gtgtcaaaca
     2461 gccctaaaaa gcgcacctct cccgccagtt tcccgtggtg cccggtcagg tctgtccctt
     2521 tgtctattgc cgccgctatc gccgcatcct ggttcatcac ccggttggca aaatgccgtg
     2581 cagccgccgc gccgcggctg aatccgaaca cgtcaaactg caccttcgca atcgccactt
     2641 tgtcacgatt aagtttgata aagcgggtca gttctgcgct gatcttctcc agtgcttcat
     2701 cggtctttgt caccacccct tcaaactgtc ggataaggct gctgcccagc gctttaccca
     2761 gtatcgaatc actttctccg tctgccgtcc cgatgccgct gatataggct ttgacctgcg
     2821 cctcagtctc gctgtcgtta agttcatcgc tttgctgata aagatcgaac agccgggaaa
     2881 tattagtgtg gccgccctgc cagcttgcat tacccagccc agctctggcg cgcttttcat
     2941 acttctcaca tgccctagcc cgctcctcgt caaccagatt ggcacagtgg gcatatgcca
     3001 gccgtagatc gtctgcgttc gcccggttat tccccgtccc gtcaaagaac acccccaccg
     3061 tcagcgttat ccccctgacc ggcacccgcc gacactggat ggttttaaaa ctgttgtgta
     3121 tgctggttcg atgggtactg tccccaataa tttcataccg gtaataacag gtatcgcccg
     3181 gcacattttt ttcatcttct tcgaccataa aaatccttat ttatctgaaa gataaaatca
     3241 gtagcaaaat ccttcgggac ttttttacat ctgcctgtta gacgtattta ccataatccc
     3301 ttaattgtac gcaccgcaga aacgcgctca gcgcgattct ggcgcaaaaa ccgcaaaaat
     3361 agatgttttc ccccgggtaa accggaaaaa tgcgtcagga ttgttttcat ggcactgcat
     3421 gactatgcat gaaactgaat ggcgatcggt ttgggcgcgc ctgatgccca taaggcgcat
     3481 tttcggacgt tttcagccct gatgagaaga aatcagacag tagttacaga cgagtcgtga
     3541 gcgattcacc ccgggagtca tcagcgagtc atccagtatt tttcctcgcg actctctggc
     3601 gactcgcttt ttctgaacac cagagagaca gtgtgttgag tcatcgataa atcaccgacg
     3661 actcgttgcc gaatcatcca gtagtcgccg acaactcgct tttgtataaa tc
//
LOCUS       DAZGAP010000312         3652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGAP010000312 DAZGAP010000000
VERSION     DAZGAP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.314"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..305
                     /locus_tag="ACHLAM_005069"
     CDS             <1..305
                     /locus_tag="ACHLAM_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7856445.1"
                     /translation="RCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSI
                     LDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            422..523
                     /locus_tag="ACHLAM_005070"
     CDS             422..523
                     /locus_tag="ACHLAM_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856446.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            520..975
                     /gene="ybcN"
                     /locus_tag="ACHLAM_005071"
     CDS             520..975
                     /gene="ybcN"
                     /locus_tag="ACHLAM_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO7856447.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            975..1145
                     /locus_tag="ACHLAM_005072"
     CDS             975..1145
                     /locus_tag="ACHLAM_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7856448.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1138..1428
                     /locus_tag="ACHLAM_005073"
     CDS             1138..1428
                     /locus_tag="ACHLAM_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO7856449.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1425..1787
                     /gene="rusA"
                     /locus_tag="ACHLAM_005074"
     CDS             1425..1787
                     /gene="rusA"
                     /locus_tag="ACHLAM_005074"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO7856450.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1784..1924
                     /locus_tag="ACHLAM_005075"
     CDS             1784..1924
                     /locus_tag="ACHLAM_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO7856451.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            2010..2393
                     /gene="quuD"
                     /locus_tag="ACHLAM_005076"
     CDS             2010..2393
                     /gene="quuD"
                     /locus_tag="ACHLAM_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO7856452.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2582..>3652)
                     /gene="ompC"
                     /locus_tag="ACHLAM_005077"
     CDS             complement(2582..>3652)
                     /gene="ompC"
                     /locus_tag="ACHLAM_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7856453.1"
                     /translation="TVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSS
                     NDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLK
                     FGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFG
                     LVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYG
                     NNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQY
                     QFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKS
                     DFTKASGVATDDIVAVGMVYQF"
BASE COUNT         1011 a          806 c          870 g          965 t
ORIGIN      
        1 gtcgttgtta taacctgctg ctatccgaac ccggcacaaa atggacagca aacaaggttg
       61 ctcgatatct ctacatttct gtttctacat tacatcgccg tctagcaagc gagggggtaa
      121 gtttccaaag tatactggac gatgtgaggt taaataatgc gttgtctgct atacaaacga
      181 cggtaaaacc tataagcgag attgccagag aaaatggtta taagtgtcct tctcgtttta
      241 ctgaaagatt tcataatcgt tttaatataa caccaagaga gataagaaaa gcttccagag
      301 agtaaaagtg ttttaagaag gagcaattct atcgattttg attttgggaa atcaacacgg
      361 cataattatg tcaccggagc ctgaacaact ccggtgactt ctgcgctaaa cggggacgtt
      421 tatgcgcaca tacaatccaa actctcttct cccttcacag atgcagaaat gcacctgcaa
      481 ttctttgcat ctagcgtttg acctctgcgg aggtgaagcg tgaacctctc acaagacggc
      541 atcaaattac atcgcggcaa cttcaccgct atcggtcggc agatccagcc ttatctggag
      601 gagggcaaat gctttcgcat ggtgcttaaa ccgtggcgtg agaaacgcag tctttcccag
      661 aatgcactca gccacatgtg gtacagcgaa atcagtgaat acctcatcag caggggtaaa
      721 acgttcgcca ctccagcttg ggtaaaagat gctctcaaac acacatatct cggttatgaa
      781 accaaagacc tggttgatgt cgtaaccggt gatatcacca ctatccagtc gttacgccat
      841 acctccgatc ttgataccgg agagatgtat gtcttcctgt gtaaggttga agcctgggcg
      901 gtgaatattg gctgccacct gactattccg cagagctgcg agttccagct gctccgcgac
      961 aagcaggagg cgtaatggct acaccgctta ttcgtgtcat gaacggacac atctacagag
     1021 tatcaaatcg tcgtaagcgt aagcctgagc tgaagccatc cgaaatacca acactgctcg
     1081 gatataccgc tagcctggtt gataaaaaat ggttgcgact ggcagcaagg aggaatcatg
     1141 gctgatttga gaaaagcagc gcgtggtcgg gaatgccagg taagaatccc tggcgtatgt
     1201 aatggcaatt ctgaaacgtc tgtactggca catatccggc tggctggatt gtgcggtacc
     1261 ggtatcaaac cgccagacct gattgccacc attgcatgtt ctgcctgcca cgacgaaatc
     1321 gaccgccgca cacattttgt cgatgctgca tatgcaaaag aatgcgcgct ggaaggtatg
     1381 gcgagaacac aggttatctg gctgaaagag ggggttatta aggcgtgaat acctacagta
     1441 tcacattacc ctggcctccg agcaataatc gctattaccg ccataatcgc gggcgcacgc
     1501 acgtcagtgc agaggggcag gcataccgcg ataacgtcgc ccgaatcatt aaaaacgcaa
     1561 tgctggatat cggcctggct atgcctgtga aaatccgcat tgagtgccac atgccggatc
     1621 gccgtcgccg tgacctggat aatctgcaaa aagccgcttt tgacgcactc accaaagcag
     1681 gtttctggct ggatgatgct caggtcgttg attaccgcgt tgtgaagatg cctgttacca
     1741 aaggtggcag gctggaactg accatcaccg aaatggggaa tgaatgatgt ttgagtttta
     1801 tatggcagaa cttcttcgcc accgctgggg gcatctgcgc ttatatcgtt tccccggttc
     1861 tgttttgacc gattaccgaa tactgaagaa ttacgccaaa accctgacag gagcaggagt
     1921 atgaagtcag agataacaat caactaatac tgttttgttg atttttgctt gtaattggcg
     1981 ttctggtctg atttttgtgg agtaagttga tgcgtgatat tcagatggtt cttgagcgtt
     2041 ggggagcgtg ggcggctaat aatcatgaag atgtgacctg gtcgtccatt gccgccggtt
     2101 ttaagggatt aattacttca aaagtaaaat ctcgcccgca atgttgtgac gatgacgcga
     2161 tgattatttg cgggtgcatg gcccgtctga aaaagaacaa cagcgatttg cacgatttat
     2221 tagtagatta ttatgtagtc ggtatgacat tcatgtcact ggcaggtaag cattgctgct
     2281 ctgatggtta tatcgggaaa aggttacaga aggctgaggg cataattgaa gggatgttaa
     2341 tggcattaga tatccggtta gagatggata tcgttgttaa taactctaat taatatgcca
     2401 attgtttact aaaaattatt aaaaatgggg cgttgcaacg cccccaaaaa taaagggtaa
     2461 tatataacag aaggtttata tagttagaag caaggttgtg ctcctaaagg aagtggcttg
     2521 agggagccac ttatatgttg gggaggcaaa gcctcccgca acatatcttt tagtaatcaa
     2581 attagaactg gtaaaccata cctacagcaa cgatatcatc ggtagcaacg ccagatgctt
     2641 tcgtgaaatc gctcttatca atcaggttga ttttgtaatc aacaaaagtg gacatatttt
     2701 tgttgaagta ataggttgca cctacatcaa tatattcaac caggtcctga tcaccccacg
     2761 cacccaagtc ttttccttta gattgcaggt aagcaacgga cggacgcaga ccgaagtcga
     2821 actgatattg tgcaactact tcgaagtttt gtgctttgtt ggcaatatgg ttattaccaa
     2881 aaacagtcat gttctgggtt tcagaatagg tggtagccag atagatgttg ttcgcatcat
     2941 atttcagacc agctgcccat acttcagcat tttgaccaga tgcattcagg ctgttgttac
     3001 cgtagataac ctgattatta gtgcggtcag atttagcata ggttgcacct acaccgaatc
     3061 cttcatactc ataagtagtg gagaaaccga aaccatcacc attagcttca gttacgtcag
     3121 tgcggtcatt tttaccctga tactgagcag caaagttcag accatcaacc agaccaaaga
     3181 agtcgttgtt acgataagtt gcaacaccag tggtgcgacc agtcatgaac acatctgttt
     3241 gggtccaggt atcgccaccg aattctggca gaacgtcagt ccacgcaccg atgtcatatg
     3301 ctacaccgta gttacggccg taatcgattg agccgtagtc cccgaatttc aggcctgcaa
     3361 atgcaagacg ggttttgtct ttggaggagc cttgagattc agcgcggttg cctttgaatt
     3421 catattccca ctgaccgaaa ccagtcagtt gatcgttgat ttgggtttca cctttgaagc
     3481 caagacgggc ataagtagta tcaccatcat ctgcatcatt agaggagaag tagtgcttgg
     3541 cattaacttt cccatataga tccagcttgt tactgtcttt attataaatt tcagctgcct
     3601 gagcagacat cgccatcagt actgatgcag ctacagcaga aattgccact gt
//
LOCUS       DAZGAP010000313         3620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGAP010000313 DAZGAP010000000
VERSION     DAZGAP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.315"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..486
                     /locus_tag="ACHLAM_005078"
                     /pseudo
     CDS             1..486
                     /locus_tag="ACHLAM_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            727..1692
                     /gene="lipA"
                     /locus_tag="ACHLAM_005079"
     CDS             727..1692
                     /gene="lipA"
                     /locus_tag="ACHLAM_005079"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO7856454.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1793..1996)
                     /gene="tatE"
                     /locus_tag="ACHLAM_005080"
     CDS             complement(1793..1996)
                     /gene="tatE"
                     /locus_tag="ACHLAM_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO7856455.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2125..2913)
                     /locus_tag="ACHLAM_005081"
     CDS             complement(2125..2913)
                     /locus_tag="ACHLAM_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO7856456.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFALPQLL"
     gene            3006..3389
                     /gene="crcB"
                     /locus_tag="ACHLAM_005082"
     CDS             3006..3389
                     /gene="crcB"
                     /locus_tag="ACHLAM_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO7856457.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3443..>3620)
                     /gene="cspE"
                     /locus_tag="ACHLAM_005083"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3443..>3620)
                     /gene="cspE"
                     /locus_tag="ACHLAM_005083"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7856458.1"
                     /translation="NESKGFGFITPEDGSKDVFVHFSAIQTNGFKTLAEGQRVEFEIT
                     NGAKGPSAANVIAL"
BASE COUNT          923 a          952 c          842 g          903 t
ORIGIN      
        1 aaaatcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaccct
       61 tctgactatt ttgaagttgc atatgtccat aaaggaatcg ttaatgcagc gaaagtgctt
      121 aatctgcccc cctcggcaat ctatcagcct attcagaaac tgcgcgttat attccctgac
      181 ctattgttta ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gcatctatat
      241 gagtctatca ctcagggact tgagtccatt cttggcgcgc tggatatcga aggaagctat
      301 gataagcaac ggacgataac tattgcgccc tccccctcgg tcggggccca ggtccttcct
      361 gtcatctatc gggcgattaa aactcactat ccacagcttt tactgcgcaa cccgcccatc
      421 ggcggcgcgg aaaaccaact ctgtcagttt caaaccgatc tcatcaccga taacatgttt
      481 tgcacaggac catagatgtt atccgtaatg cattttgaaa aagtattgtt acagccagat
      541 cacagcgcag aacgataaaa tggcacaatc caacaacaat tttacatttt cgcgaccgct
      601 ttggctgctt tcaggtccgt ttcaatgata tactgccggt cgttaattca aaaatagttg
      661 ataattacaa caatctattg aattgaaacg ctttccttcg taattcgcaa ctggaacacg
      721 cacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga tgccgataag
      781 atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct gcgcaagccg
      841 gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat caaagccgca
      901 atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa cctggcggaa
      961 tgcttcaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac ccgccgttgt
     1021 ccgttctgtg acgttgccca cggtcgcccg gtagctccgg atgccaatga accagtgaaa
     1081 ctggcgcaaa ccattgccga tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt
     1141 gatgacctgc gcgatggcgg tgcccagcac tttgcggatt gcattactgc cattcgggaa
     1201 aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgcggtcg tatggatcgt
     1261 gctctggata ttctgaccgc cacaccacca gatgtgttca accataacct ggaaaacgta
     1321 ccgcgtattt accgtcaggt acggcctggt gcagattaca actggtcgct gaagctgctg
     1381 gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat ggtgggtctg
     1441 ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg
     1501 atgctaacgc tggggcaata tttgcagcca agccgccatc acctgccggt tcaacgttac
     1561 gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat
     1621 gctgcatgcg gtccgtttgt ccgctcgtct taccacgccg atttgcaggc gaaagggatg
     1681 gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc cgctttttga
     1741 agcgggtttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca cgtcactctt
     1801 tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt
     1861 catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg cccagcgtac
     1921 gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactaccagc agtttggtaa
     1981 tactaatctc acccatagat accttcttga catataaccg gcaacagaac gcgccttaaa
     2041 aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg taacaaggtg
     2101 aatcaagcgc cgagtaaaaa aacatcataa taattgcggc agcgcaaagc gacggttgtt
     2161 taagacgggc agttgcgcgc gtacctgacg cacacgttcg ggcgtcactt ccgccataat
     2221 gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta taatccggct
     2281 ttgcccgata tttttgttcc cgcactcccc cgccgccacc atataacagg tggtatccag
     2341 cacacgagcg gcaagcaacg ttgaccagtg atgctctttg agcggcccac gaacccaggc
     2401 ggcaggaagt accaggattt cagctccctg taatgcctgt gccagcgcca gctctggaaa
     2461 gcgtaagtca taacaggtca tcagaccgac cttcatccct tccacctcca gtaacggagc
     2521 tatttcatta ccagcatcaa catggcgtga ttcctgaatg gcaaatgcat catagagatg
     2581 cagtttggca taacgggcga cgatgtttcc tgcctgtagc gccaccagca tattccacgc
     2641 gcgccccggt gttgaaggaa catgaatcgt cagaattgtc gtcatcatgt tacgtttact
     2701 ttctcgccgt aaacggtcga ggaattcgcc ttccagcagc tgtgctgatt taaccgaaag
     2761 atccgcatca tgatcatcac gcgccagtaa tgcttccggc aggacaaaca gcgaaacctc
     2821 gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt cccacacaga
     2881 tgtaacagca aactgtcctg cggcaaccag cattgccacc tcctgtcaga gtcgtaaaat
     2941 tcgccaacgg cgattattcg gcgttacact tatcactcat acaaatcaaa tagcaggatt
     3001 ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga agcgtggcga
     3061 gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg gggacgctgg
     3121 ctgcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc agcaggatga
     3181 cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc ggtctaacaa
     3241 ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc tttggctggg
     3301 cgttactgaa cgttttcgtc aaccttttag ggtcttttgc catgaccgca ctggctttct
     3361 ggctgttttc cgcctcaacc gcacactaaa gaccaaaaaa aacccgctga ttaagcgggt
     3421 tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa gggcctttgg
     3481 caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta aaaccattag
     3541 tctggattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga gtaatgaaac
     3601 cgaatccttt ggactcatta
//
LOCUS       DAZGAP010000314         3612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGAP010000314 DAZGAP010000000
VERSION     DAZGAP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.316"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..171
                     /locus_tag="ACHLAM_005084"
     CDS             <1..171
                     /locus_tag="ACHLAM_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856459.1"
                     /translation="SYLSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIEDE
                     IIVCATYLIPDI"
     gene            173..517
                     /gene="ymgG"
                     /locus_tag="ACHLAM_005085"
     CDS             173..517
                     /gene="ymgG"
                     /locus_tag="ACHLAM_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO7856460.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            521..856
                     /gene="ymgD"
                     /locus_tag="ACHLAM_005086"
     CDS             521..856
                     /gene="ymgD"
                     /locus_tag="ACHLAM_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO7856461.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(914..3556)
                     /locus_tag="ACHLAM_005087"
                     /pseudo
     CDS             complement(914..3556)
                     /locus_tag="ACHLAM_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
BASE COUNT          945 a          866 c          785 g         1016 t
ORIGIN      
        1 agttatttat catttgagat tattcttatt cccgtaaaaa acataattcc tatcatcaca
       61 gtcacgctca ttttgaatta tctgaataat agcgagcgtt ctttagttaa gcagatctta
      121 attgaagacg aaattattgt gtgtgcaact tacttaatac cggacatcta agttgaagaa
      181 aaaaatactt gccttcgggc ttatctcagc attgttctgc tccacgccag cgatggcgga
      241 tatgaaccgc accacgaaag gtgcattgct aggcgcaggc gttggtttac ttactggcaa
      301 tggtgttaat ggcgtactga aaggtgcagc tgttggcgct ggtgttggtg cagtaacaga
      361 aaaaggccgc gacggtaaaa atgcgcgtaa aggtgcaaaa gtgggtgccg ccgttggtgc
      421 tgtcactggc gttctgaccg gcaatggcct cgaaggggcg attaaaggtg ccgtgattgg
      481 cggtacgggt ggtgcaattc tgggtaaaat gaaataagga atgatgatga aaaaatttgc
      541 tttactggcg ggtctgtttg ttttcgcacc aatgacctgg gcgcaggact acaatattaa
      601 aaacggtttg ccgtcagaaa cctatattac ttgcgcagaa gccaacgaaa tggcaaaaac
      661 tgacagcgca caggttgcag aaattgttgc ggtaatgggt aatgccagcg ttgccagccg
      721 tgatttaaaa attgagcaat ctccggaact aagcgccaaa gtggtagaaa aactgaatca
      781 ggtttgcgct aaagacccgc aaatgctgtt gatcacggca attgatgaca ccatgcgtgc
      841 tatcggcaaa aaataatatt tcatatgtta ccgcgtcgat gaattcgacg cggtttttta
      901 gctaatggcg ggatcagaac ttatatttca ggcccaccat cattgcggtg tcattgtagc
      961 cattatcccc cagctgcacg cccacattac cccagacgct ggccgccgga ttcagattgc
     1021 catttacgcc aagtttaatt tcccccagat tacgcgcccc gtcttgttca actttcacac
     1081 cgttcattga gacagcaaaa tctttactgt tatggatcca gttcatttca acaaaaggtt
     1141 caaagtcgtc accgtgcgga ccgctgtttt tctcctgagt acgaataaag gtgcggaagc
     1201 cgagtcgggt ctggatgttg ttatttgcgt cattctcaat acgtgagcca ttggcttccg
     1261 tgtgcgcatt atgatcgacg cccatccaga tagcctgtgg ctggaagagc cacgtatatt
     1321 tcgccgcttc cagaccaaag gatttattca gggcaaaacc gtaacccgct tccagcgaga
     1381 cggttgcacc tttcgcgcta taggattcat aggctaattc atcccctttc accgagtttt
     1441 taaaccagct atattacgcc caggtgtcaa tgtatgcgcc ttttttactg atgtcatcgg
     1501 caaaccaggt ggcatacagc cctgcgctat agcctctgac actccctttc gaacgataat
     1561 ccgacacgct ggaatgagtt gagttgtagt cgcgggcata accagccaac actcccaaac
     1621 gccaactgtc actgtcggta aaaccaccgg ttaacagatc cgcccctaac tgcgagacgt
     1681 agcgatgcga ggtcgttctc aactgtccgt tgctgtcacg ccaggcatta tgcccgccaa
     1741 tttgacgtag ccaaagccgg cttgaacgct cctgttaagt tacaggatcg atgtaacgcg
     1801 tttcacctgc acgatcgttc agacgcataa caaaaagagt gttagcggct gccaggttgg
     1861 cgacgtaact tccagcttca gggcgcagga cgggggtagg cgcaacagta ggcgctgaac
     1921 ttcctccgga gtttgtttca ccgccgctcg ttgcctggaa gttagtcaga taccagtttt
     1981 tattggtcgc ttcggtatta cctcgggtta atgaatattc gtaggcaccg gcgaaaatcc
     2041 tcgaatcctt aataaattct ccatttgatt ccccctcaac gctgataatc tctatcccat
     2101 ttaacgtgta agccccgctg ccgccagcat tggtaacttt aactcgggtt gttccagagg
     2161 tatttccatt aataacgact ttatccgtag ctgaattatc atcacccaat tcagttctga
     2221 gatgcaatac gccattatta ccaacgtaat tttcagttat tgtcaatctt gttggttcga
     2281 aatcctttcc atctgcccgg gaaatataga ccgtactatt atcaaccgtg agtttgctca
     2341 ccgttgagtc agctttaata tcccaggtac tattatctga aagcgacaga taggtatgag
     2401 tgttatcatc tgttgaaaga ttggctgaac ccgttatttt tgaattagtt gcatcgacgg
     2461 tcaaatgacc tttaatatta ttaataaaaa catctgcagc cgatgatacc tcactatccg
     2521 taatggtcac gttactcgtc tcatttttat ggtagaggat ttcatttgct gaccgtagtg
     2581 agctaccaga taaaatatct accgttgctt cctgaccatt tatggaagac tccagcacaa
     2641 ttccagcccc ctgatgcgcg actgcagatg tatttttcag cgtcaccttg ccgccattat
     2701 aaattttagc aatcgcggcg tcaggacccg ttgtataaat ttctccctgg ttaaatgaaa
     2761 ctgtcccctg gcgagcagcg atggcatgag cattgtcccc atgcgtggtg atatcaacat
     2821 cggttaatgt aacgctggca ttggtataat caagcttgct accctccttg acgacagtgc
     2881 cattagcgta agcggcataa ctttctgttc cgtgggttgt tatctcccct tttgccatat
     2941 ccagttttgc tttggcgtta ttttcattac ttcctgtatc ggcagtactt tcttcatcaa
     3001 gagaagatac acacgcacgc gcgcttccgg gagatgcaca cgcaagaacg ccgactgcgt
     3061 gattacccgc agtaacgata aaagtattac ctccgtcaga aaaagagggg gtagccgaac
     3121 cgtcagggtt gactcttatt atggtatcag tattcaggtt tccatcaact tgacttaaca
     3181 aaccaacaga ataatctccc gtcgtgacaa tcgtattatt accccgcaaa gtgacagcac
     3241 cgccatattc acttacgact gcgctagctg tatatgctgt gcttagagaa ctattgtcag
     3301 aaggatataa accgcccgtt gttacaatga cgccattgtt aatggttaaa ttgctaccct
     3361 ttcctgcaac agatacaccg taacttccat tacccgttgt tataactagc gcattatcaa
     3421 tatcttttac ttctccatct tttatttcaa caacatgttt aggttcatcg tgctgaataa
     3481 actgttcagc atatgcatta cccaccgcca aagcaataag tcctaaacta aacccgggga
     3541 gttttttcag tttcatagat taaccatcca ttcattggat taacaaacat tcctggtcaa
     3601 ataacctatg ag
//
LOCUS       DAZGAP010000315         3599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGAP010000315 DAZGAP010000000
VERSION     DAZGAP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.317"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(108..1427)
                     /locus_tag="ACHLAM_005088"
     CDS             complement(108..1427)
                     /locus_tag="ACHLAM_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO7856462.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1558..3093)
                     /gene="gadC"
                     /locus_tag="ACHLAM_005089"
     CDS             complement(1558..3093)
                     /gene="gadC"
                     /locus_tag="ACHLAM_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO7856463.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3249..>3599)
                     /locus_tag="ACHLAM_005090"
     CDS             complement(3249..>3599)
                     /locus_tag="ACHLAM_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO7856464.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
BASE COUNT          939 a          890 c          902 g          868 t
ORIGIN      
        1 ttgcgtagtt aatttgactt aagtaagcat gattactagt gggatatttc aagagggtaa
       61 caagccgggg ggaaagcacc ggcttgttac aaagtaagaa tgggagttta actgccccaa
      121 cgactttgca gatagctgac cgcttgttga gtctgtggct tattcagata gtcctcacgg
      181 aacaagattg tgccgctaat ttctggcacg gcatcgttaa gatcgagctg ctttttcagt
      241 tccggtacgc cgccgttaat catccagtct ggctctatct ttgaaggttc acccacttta
      301 tagaaggcga taccgatata caggcgggtc ctggtcggtt taacgacatc cgcccaccat
      361 tttgccaaaa cgtcataacg tgcggcactc cgtgagaacg gccagtaaat ttggggagca
      421 atgtaatcca gcaatccttg ttccacccat cgacgggtgt cagcgtagga ttcgtcatag
      481 gctgccgcgc ctcgggtatc ggaaccgagc ggatcgtgtg atcggttacg ccacacgcct
      541 gccgggctaa cgccaaattc gacttccggc ttaatgcttt taatagtgtg cgataccttt
      601 gcaattaact gctgagtatt gttgcgccgc cagtctgctt ttgacgcaaa tgcgcctccg
      661 tatttgcggt acgtttcgtt atcatttagc cgtgaacccg gtgactcagt atagaaatag
      721 tcgtcaaact gcacgccatc taccggatag cgggaaacca cttccgcgac tattgatgtg
      781 atccagtcct gaacttcagg gatgcccggg tcgaggacaa agcgatcgcc ggacgttctg
      841 atccagtcgc ggtgttgcac atagacgctc gctggttgtt gagacagagt gctattcagt
      901 tccctgatag taccgggctt cgtattaacc gatacgcgat aggggttaaa ccaggcgtgt
      961 actttcatcc cacgcttgtg ggcttcgtcg agcataaatt gcagcggatc gtaacctgga
     1021 ttttcaccaa tctttccggt cataagatcg gaccacggca aaatttttga tggccacagg
     1081 gcggtaccgt ccggcttgac ctggaaaaag accgtgttga tgccgagacg ttgcagatga
     1141 tccagtttgt cgaccatcgc ctgttgttgt acacgggccc ggctggtggg gttactaatg
     1201 ttaaccgagg aaaccggtgg ccagtcgagg cgagacactg tggccagcca gatgccacgc
     1261 atcggttgtg acgattgttg cgtcgtagct ggtggcttcg aacctgttgg tggtgtcacc
     1321 attgactctg gaggcgtgct tttgcaacta cacagtaaaa gtgccagtgc aaccagtatc
     1381 gctggtcttc taatcgctaa tttctcgttt cgggagcaga tatccatatg tgctggtttc
     1441 cggtaaacag atgtgcgctc attctcgtac ttatcccggt taagtcaata cgacaacaag
     1501 cacgaaaaag ggagcgatga attatcgctc ccttgtctta taaccattca gacatggtta
     1561 gtgtttcttg tcattcatca caatatagtg tggtgaacgt gcacgcgggt gcaggaagaa
     1621 gtgacctttt ggtgcgttct gactgttgat tggctccaga gtgacgccgg tattcgcttt
     1681 gcttttacga tcatgaacag catagagaat aaagggcagg gcaagtacca ccaggaaact
     1741 cacgaccagt aattcaacat acatatcggt agagtcaccc tggatgttat ccggcggcag
     1801 gaaggaaaca ataaacgcca taattgaagt cagcagaccg acaatagcca cgaccagttt
     1861 caccccttta ccaccaggga tattaaatgt gcgttttaag tcaggatgtt taagaaccaa
     1921 cacaatgtag ccaataaaca gcatgaaata agcacacaga taaatcacca ccgtcagcgc
     1981 cagtgcgatc aggaaggaca tgttgttacc gccaccggta ttggtgagga tgatcaacgc
     2041 gatagaagta atcaccaact gcgaaatgac cagcgttacc ggtacgccat ttttgttcat
     2101 tttagcgaat gccgctggca gcaggttttt ctgcgctgtg acatacatcc cgcgagaagg
     2161 accaacaatc caggaagcga tttccgccag aacacccagc aacagcagtg cggagatcac
     2221 gcgaaccgtc cactcaattt ccggtgccac atgtgacatc agaacggtaa aggtttgcat
     2281 tacccctgcg gagaggttga tttcattacc cggaatgacc atcgcaatag acaaaccgcc
     2341 aacagagctt aagcagattg ccgccaccat cagcagtaac atagccagtg gatagtcgcg
     2401 cccgggattg ctcatttcat tgacgtgggt tgcggaagct tcaacgccca tataactcaa
     2461 aatgaaggca acaaatacaa ccagggtgcc cactttagag aagtcaggga agaaggtctt
     2521 cgaatccatt tcgatagcaa cgggggcacc ggagtgcaga taaatagccg ctaatgcgat
     2581 caaaataaat gcaggtaaca ggataccggc gaagaagcca actttagcaa ttcgcgccgt
     2641 gtatttcgtg ccaccaaact gcgttaatgc cagcgcccaa agaatgatga gtgctgcaat
     2701 agttttggta atggggtctt cattcagcgc tggccatttc aggatgtagg agagtgcccc
     2761 taacacgaaa tagagcatcg gaataaaacc aatggcgatt tgcagatagc caaatgagat
     2821 cgctgcaaat ccccatctcg gccccagagt atttgatacc caggcgaaga cgccaccttc
     2881 ttcccagccg tcgacggtag ccatttccgc agcacaaagt cccacgggaa taaaccataa
     2941 aatcccgcct aatagcagga agaagactaa tgaaaagccc gatgttgcga aggtagggta
     3001 ttcataaaca gccattacca tcgatgccgt tatggcaaaa aatccgagta atgtgagctg
     3061 cttagcttta cctgtctgta ctgatgtagc catattatcc ccctaaaacg gtattcctgt
     3121 cggaaccgca cctgttttgt tctgaagcgt attcagaaca atattttccg ttgctaatgc
     3181 cagtgaacag actttggaaa ttgtcccgaa acggcttcgt ttcgggacac cgttaccgtt
     3241 aaacgttatc aggtgtgttt aaagctgttc tgctgggcaa taccctgcag tttcgggtgg
     3301 tcgctgagat atttcaggga ggctttgtag tcttccagca acagttcagc aaagtccatt
     3361 tcgaagccgc gacggcacat aatgcgcatc accacgatgt cggtggcttc accgcctaga
     3421 gtgaaagccg gaacctgcca gccgcgcaga cgcaaacgtt cagagaggtc atacagggtg
     3481 tatcccggat cttcaccatc tttcagtttg aagcaaaccg ccgggatgcc ttcgtccggg
     3541 cgacccgtac agatgaactc atacggcccc agtttggcga tttcatccgc cagataagc
//
LOCUS       DAZGAP010000316         3516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGAP010000316 DAZGAP010000000
VERSION     DAZGAP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.318"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..986
                     /gene="allS"
                     /locus_tag="ACHLAM_005091"
     CDS             60..986
                     /gene="allS"
                     /locus_tag="ACHLAM_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO7856465.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            1055..2149
                     /gene="mnmH"
                     /locus_tag="ACHLAM_005092"
     CDS             1055..2149
                     /gene="mnmH"
                     /locus_tag="ACHLAM_005092"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO7856466.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVKGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTR"
     gene            2265..2630
                     /gene="ylbG"
                     /locus_tag="ACHLAM_005093"
                     /pseudo
     CDS             2265..2630
                     /gene="ylbG"
                     /locus_tag="ACHLAM_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3397..3516)
                     /locus_tag="ACHLAM_005094"
                     /pseudo
     CDS             complement(3397..3516)
                     /locus_tag="ACHLAM_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          900 a          838 c          884 g          894 t
ORIGIN      
        1 gataaatcag aggataagaa aatcacgcaa cttgcggatt taaaagggga tacctgaaga
       61 tgttcgatcc agaaaccttg cggactttca ttgcggttgc tgaaacagga agtttttcaa
      121 aagcggcaga acgattatgt aaaaccacgg cgacgatcag ttatcgcatt aaacttctgg
      181 aagagaatac cggagtagcg ctgtttttcc gtacgactcg tagcgtgacg ttgacggcgg
      241 ctggcgagca tctactttgt caggccagag actggctgag ctggctggaa agtatgccca
      301 gcgagctgca acaggtgaat gatggcgtgg aacgccaggt gaatattgtc atcaacaacc
      361 tgctctataa cccccaggcc gtcgcccagt tgctggcgtg gctgaatgaa cgttacccct
      421 ttacccagtt tcacatctcc cgacaaatct atatgggcgt ctgggattcg ctattatacg
      481 aaggtttttc actggctatc ggcgtcacgg gaaccgaggc gctggcaaat acctttagtc
      541 ttgatccctt aggatcggtg caatggcgct ttgtcatggc ggcggatcat ccgctggcga
      601 acgttgaaga gccgctaaca gaagcacagt tgcggcgctt tccggcggtt aatattgaag
      661 acagcgcccg cacactaacc aaacgcgtcg cctggcgatt gccggggcaa aaagagatta
      721 ttgttcccga tatggaaacg aaaatcgccg cccatctggc gggcgttggc attggttttt
      781 tgccaaaatc actttgccag tcaatgatcg ataatcaaca actggtcagc cgggtaatcc
      841 caacgatgcg ccctccttca ccattgagtc tggcatggcg caaatttggc agcggcaaag
      901 cgatagaaga tattgtgacc ttgtttaccc agcgcaggcc ggaaatcagc ggatttttag
      961 aaattttcgg caacccacgc agttaaaaat caagtttcct tgcgcgttgg cgcgtaaaat
     1021 agcgcgcaag ctgatttgag gttcactttt tactatgcaa gagagacaca cggaacagga
     1081 ctatcgtgcc ctgctgattg ctgatacgcc cattattgat gttcgcgccc ccatcgagtt
     1141 tgagcaaggc gcaatgcctg ccgctatcaa cctgccgtta atgaataacg atgaacgcgc
     1201 cgccgttggc acctgctata aacagcaagg gtcagacgcg gcgctggcgc tgggtcataa
     1261 actggtggcg ggtgaaattc gtcagcagcg tatggacgcc tggcgggcag cgtgcctgca
     1321 aaatccgcaa ggtattctct gctgcgcccg tggcggtcag cgctcacata ttgtgcaaag
     1381 ctggttgcat gcagcgggga ttgattatcc gctggtgaaa ggcggttata aggcactgcg
     1441 ccagaccgcg attcaggcga ctattgaact ggcacaaaaa ccgatagtgc tgattggcgg
     1501 ttgtaccggc agcggtaaaa cgctgttagt gcagcaacag ccgaacggtg ttgatctgga
     1561 agggttggcg cgtcatcgcg gttcggcgtt tggtcgcacc ttacaaccac aacttagcca
     1621 ggcgagtttt gaaaacctgc tggctgccga aatgctaaaa accgacgccc gtcagaattt
     1681 gcgcctgtgg gtgctggaag atgaaagccg gatgatcggt tcgaatcacc tgccggaatg
     1741 cctgcgcgag cgaatgaccc aggcggcgat tgcggtagta gaagatccgt ttgagatccg
     1801 tcttgagcgg ctgaacgaag aatatttcct gcgtatgcat catgatttta cccacgcgta
     1861 cggcgacgaa cagggctggc aggagtattg cgaatacctg catcacggac tttcggcgat
     1921 taagcgtcgg ctggggctac agcgctataa cgaactggct gcaaggctgg atgcggcgct
     1981 gacaacgcaa cttaccaccg gcagcaccga cggtcatctg gcctggctgg tgccgttact
     2041 tgaagaatat tacgacccga tgtatcgcta tcagcttgag aaaaaagcgg aaaaagttgt
     2101 ctttcgcggt gagtgggcgg aagtggcgga atgggttaag acgcgataat gagggacgta
     2161 tttgtattag tcgcgacttg atctgacaca tggccttgaa aggttgagag ttaccggttt
     2221 tgatatgagt gtcgaatcct tatccaaaac aagaggtaac tctcatgctt catactgcca
     2281 atcccgtcat caaacacaaa gccggtttgc tcaatctggc tgaagaactc agcaacgtat
     2341 caaaagcctg taaaatcatg ggcgtttcgc gtgatacgtt ttatcgttac cgcgaactgg
     2401 ccgctgaagg cggtgtggat gcgcagatta atcgtagtcg ccgcgctcct aaccttaaga
     2461 accgtaccga tgaggcaatt gaacaggctg ttgttgatta cgccgtcgct ttcccgacac
     2521 atggtcagca ccgggccagc aacgagctgc gtaaacaggg ggtttttatc tccgacagtg
     2581 gtgtccgttc tgtctggtta ctccataacc ttgagaatct caaaagacga tactgaacaa
     2641 attttatcag gtgacgttcc gtaaaaagtt gtatggagtt ctcgatgcag tcggcgcgac
     2701 tatcagtacc cccgctgaac cctggactat cgggccgccg tactggggca tgtcaccctg
     2761 gcgtgccgct ggttttccgc taatggttta tttcctcttt ctttcccggc gagaatttat
     2821 tttaatccgg aaggattatt tattttcaga tagggactca cattaaacac gtaacatttt
     2881 aattaatgca tggctgaata tgtattacac aatgtcagat atcgtaaaag accagtgcaa
     2941 ttctatgtaa actctctggc tgttcatttt atttgttgtt tcagggtcgg aactcaccct
     3001 caataaacaa ctgaatacga gtagatattc atcgtctgag ctatatggct ttacacaata
     3061 gccgacatgt cttttctcag tcagggatta tacttttcct cactacgaag ttgacgactt
     3121 cgatatggga tagactctta attcaagcaa ttactctgca caagcatcag gtaacccagc
     3181 tccaatattt gttataaaat aatgcgtaac attaatttca ccaacaaaaa aaaggtttac
     3241 gctatcccct tgccctggta ttttaccata tttacaccac actttttttg tatctgtatc
     3301 attctctgat acgattttcg ccacttgttc cctggccctc acagtcaatg ttttcttaaa
     3361 cctcccccca tcatcgattg ttagggaaaa ataagaaaaa aaataatcat cttctacatt
     3421 tgtggatatt gatatatcca ccgctacgtg cggaaaaaaa tctgggagcc atgtaaaaac
     3481 cccatcactt tctggtattg ccatatagtt tatgta
//
LOCUS       DAZGAP010000317         3481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGAP010000317 DAZGAP010000000
VERSION     DAZGAP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.319"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(329..2371)
                     /locus_tag="ACHLAM_005095"
     CDS             complement(329..2371)
                     /locus_tag="ACHLAM_005095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO7856467.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLTRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQDACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQFFDLSLLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGSLITAMKNASSGVT
                     DPVLKKILRDNAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(2374..3078)
                     /locus_tag="ACHLAM_005096"
     CDS             complement(2374..3078)
                     /locus_tag="ACHLAM_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO7856468.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            3124..>3481
                     /locus_tag="ACHLAM_005097"
     CDS             3124..>3481
                     /locus_tag="ACHLAM_005097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856469.1"
                     /translation="MRITDNKKTNQAEYTFNAAYFLIAVATGNTAHFLTSVATGSTAH
                     FLISAATGSTVHFSTSAATGCTVRFLTSAATGTIGYFLYYGTTGTIDYFLYSGATGTI
                     DYFLYSVATGTIGYFLY"
BASE COUNT          819 a         1021 c          743 g          898 t
ORIGIN      
        1 gtagccaccc gaaggcgctc ctctcaggcc acttgtggag cattggtggt cgccggagcg
       61 acgttatgtg cccggacaga ctgaaatcac tgatggaaaa agtcaacagc taaccaaaaa
      121 caaaaaagca ccggcaagcc ggtgcttcag ggggatacca aatcccggtg ttgattctca
      181 tcttctccgg agagagcgac tcccggcacg gttttcacgg agtgcatgtg ccttgtaacc
      241 ccgccgtgtg cataacgggt taccggacct ctcagggctg tcagccccac tgtggtcaac
      301 agggtgatac cagaactcgc ccgcaatttt aattcagttt gtctccgaca tcaccagatt
      361 tttgtttatg agaaaaatta atttttcttt tcctgcccct gctgtttccc tgctcaccag
      421 caattcccct gcagtcggga taacgggagc atccccagaa aaaaccgctt tttcccttcc
      481 gtttcaccgt cggaccgtta cagagtggac atgaaggacc tgattccact gacagattaa
      541 ccgtctggtt acgtccggat aacaccaggt gcctgatcca ctgatgctgt ttctccataa
      601 acgcctccag tgagattttc ccccgggcaa catcatccag cgcctgttcc cataaagccg
      661 tcatacccgg atttgtcaga gagtccggca atgccgccac cagttctctt cccagttgcg
      721 tggaacggat atgttttccc gctctctcaa tatagttgcg cgcaaataac gtttccagga
      781 tccccgcgcg tgtggcttcc gttcccagtc ccgcattatc cctcaggatc tttttcagca
      841 ccggatccgt tacaccagac gaagcattct tcatcgcagt aataagactg ccttcagtaa
      901 aaggacgggg aggtacggtt ttcagctcct tctgttcagc accatccaga gcacagagtt
      961 ctccctttat caaagggggc aatatgtttc cactttcatc actgtcaggc ttttcttcat
     1021 ctgcatgatt ttccggtgga aataactgtt tccacccgat aatcacattc actcttccgc
     1081 gggaacggaa taactgcccc ccaatcacaa atgttgcctc agtaacatcg gcttcctgct
     1141 ccggcagaaa ctgtgccaga tagtgctgac gaatcagttg atataccttc agttcatcac
     1201 cgttcagtaa gctgaggtca aaaaactgcc gggtcggaat aatggcatgg tgagccgtaa
     1261 tttttttatc attccagata cgggataccc gtcctgattc cagttcagcc agttgtggaa
     1321 gcacggacgg atctgaacgc ccgacagcct caagcacttc agaactctcc tcatgcatcg
     1381 aggcaggcag ataaccacag tctgtcctgg gataagttgt tgctttgtga gtctcataaa
     1441 gggactgtgc gatatccaga acggtctgag cccccatgcc aaacttccgg gaacaggcgt
     1501 cctgcaacgt tcccagcgaa aaacacaaag gtggcggtgt tttctccctc ttctgtacaa
     1561 catcctgtac aacagcagag gactgctgac ggcacagacc gagaacagca ccggccgcct
     1621 gcacattcac acaacgtccc tcatcatcgc agtactgctc agctgccacc cagttactgc
     1681 ggaaacggag cccctccttt tccagccaga caatcacctg ccaccaggaa accggcacaa
     1741 atgtggcgat ctcctgatcc cgtctgacca ccattgccag cgtgggtgtc tgcacccggc
     1801 ccactgacaa aaccccagaa attccacgtt cccgtgcctt cagggtatac aaccgggtca
     1861 gattcatccc tgtcagccag tcagcccggg cacgcccctt accggcttca ctgagaagcg
     1921 cagtctgttc gcccgggaga atattattca gcgccttcct gatactggcc tcatccagtg
     1981 cagataacca cagacgcctg acacgccctc tgtactgaca gtactccagc aactctctgg
     2041 ctatcacttc tccttcacga tcggcatctg tggcaataac cacttcagaa gcgcgtttta
     2101 acagcccccg gatcacaata agttgttttt tcaccttcgg cttcaccacc atctgccatt
     2161 gttccggtat cagcggcaac acatcagaac gccatgggga accgaactgt tcaccgtaag
     2221 cttccggctc agccagttcc agcaggtgtc ctatcgccca ggtgacagca atattgtcat
     2281 ctccgatgta tccgtcgccc cgcttactga gcgccagttc acgggcgata tctttcgcct
     2341 gagaaggttt ttcacacaga aagagttgca tactcacccc gctttaaccg gtggtgaata
     2401 aacagaacgt ttcttaccgc tcatcacgtc aggatccggc tcccccatgc ggttaaccgc
     2461 ctcctttcca cgggctgtgc cggacaaaat atcagcacgt gtaacactga ctgatttata
     2521 attttgaata atcccgtaaa tcctgcgtac cgcataccct ccctgattca gcaactcatc
     2581 ccgttttgtg cgggaaataa gtccgtaatg aaatacctga aataccttca tgaccagtcg
     2641 atcatatccg atcagaagcc atacacatcg ataaccgacc ggagaactac tgtacacgcc
     2701 aatatttacc ggcgaagagg atttaatatc agaaagggag atctgtcccg gcaggttttt
     2761 aaggatcccg tcaagccacg caagatgctt ttcaattgtt tcagttgcct tcgtcagggc
     2821 attttccagc ctgactaaca tcatatctgc ataaggatta tccctggctg aatctctggt
     2881 tgcctgaccc gcaaacgcaa tagccctggg catactgatg atgtcaggcc gcttttcctg
     2941 tttttcttcc ttctgattat tcctgcgtcc ctcccagaga cggatcgcat atcgggtatg
     3001 aagggcaata gtcagcgatg actgcagagc accaacttgt tctgattttc tgacttctcc
     3061 ggttttctcc tggggcattg tgtttactcc gaatgatttc atgaccagaa gatagtccgc
     3121 aggttgcgta ttaccgacaa caaaaaaaca aatcaggcag aatatacatt taacgccgct
     3181 tattttttaa tcgctgttgc cactggcaac accgctcact ttttaacctc tgttgccact
     3241 ggcagcactg ctcatttttt aatctctgct gccactggca gcactgttca cttttcaacc
     3301 tctgctgcca ctggctgcac tgttcgcttt ttaacctctg ctgccactgg caccattggc
     3361 tattttttat actatggtac cactggcacc attgactatt ttttgtactc tggtgccact
     3421 ggcaccattg actattttct gtactctgtt gccactggca ccattggcta ttttttatac
     3481 t
//
LOCUS       DAZGAP010000318         3480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGAP010000318 DAZGAP010000000
VERSION     DAZGAP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.320"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..658)
                     /gene="hprR"
                     /locus_tag="ACHLAM_005098"
     CDS             complement(<1..658)
                     /gene="hprR"
                     /locus_tag="ACHLAM_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO7856470.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     V"
     gene            791..1204
                     /gene="hiuH"
                     /locus_tag="ACHLAM_005099"
     CDS             791..1204
                     /gene="hiuH"
                     /locus_tag="ACHLAM_005099"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO7856471.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            1313..2317
                     /gene="msrP"
                     /locus_tag="ACHLAM_005100"
     CDS             1313..2317
                     /gene="msrP"
                     /locus_tag="ACHLAM_005100"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO7856472.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTHERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            2318..2953
                     /gene="msrQ"
                     /locus_tag="ACHLAM_005101"
     CDS             2318..2953
                     /gene="msrQ"
                     /locus_tag="ACHLAM_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO7856473.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            3210..>3480
                     /locus_tag="ACHLAM_005102"
     CDS             3210..>3480
                     /locus_tag="ACHLAM_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO7856474.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTF"
BASE COUNT          934 a          831 c          769 g          946 t
ORIGIN      
        1 ctacgaatga atatcccatc ccacggattg tggcaatgag cttttcagga aaaggatcat
       61 caacttttgc gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt
      121 tgattcccca aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg
      181 ccagtagcca aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc
      241 tcacactttg actaacagag tccattctta agccactgat ttctaatgtt gaattcaaag
      301 cgtgatgttg ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt
      361 ttaccagata atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc
      421 ttgctgtaag gcaaataaca ggagtttgct ttgccgttct taacgtttgt aagatctgcc
      481 agccatccat acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg
      541 caagataaag cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa
      601 gcccctgcgt tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga
      661 aaataatatg ccataaatca tatgttatag aaacagcctg gttcattaca aaattgtaat
      721 gctgctgtaa ggttaccctg gacgcttttt cgctatcctc aaaactcatt cacatgacaa
      781 ggatataaac atgttaaagc gttatttagt actctccgta gtaacggctg cattttcatt
      841 accttctttg gtctatgctg cacaacaaaa cattcttagc gtgcacattt tgaaccagca
      901 aacaggaaaa cccgctgccg acgtgacagt cactcttgaa aagaaagcgg ataacggctg
      961 gttacaactt aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga
     1021 gcaaactgcg actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa
     1081 acaaaatctt gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa
     1141 cgagcattat catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag
     1201 ttaatgcata aatatataaa agcagatctc tgctactgtt agggaggtga ctccctgaca
     1261 gattaacagt aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa
     1321 gaatcaattt ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg
     1381 gcaggtgtta aaagcactgg gcatcagcgc ggctgcacta tctttgcctc acgctgcgca
     1441 tgccgatctg cttagctggt ttaaagggaa cgatcgtcca cccgcccccg ccggaaaacc
     1501 gctggagttc agcaagcctg ccgcctggca aaataacctg ccactgacgc cagcagataa
     1561 agtttccggt tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa
     1621 tgctggtagc ctgaaaaccg atccatggac actgaaaatc agcggcgaag tagcaaaacc
     1681 attaaccctc gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg
     1741 tatgcgctgc gtggaagcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa
     1801 attgctggcg cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta
     1861 tgcaccggaa cagatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc
     1921 ttatgtcgaa ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtagg
     1981 tgtttatggc aaggcgttac cgccacaaaa tggcgcgccg gtgcgactga ttgtgccgtg
     2041 gaaatatggc tttaaaggga ttaaatcgat cgtcagtatt aagctgaccc acgagcgtcc
     2101 gccaaccacc tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc
     2161 gcatgttgat cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat
     2221 cctcgatgta cagcgccagc caacgctact gtttaatggt tacgccgagc aggtggcatc
     2281 gctgtatcgt ggcctggatt tgcgggagaa tttctaaatg cgtctgacag caaaacaggt
     2341 gacatggctg aaagtttgcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg
     2401 ggcgattaat cacggtggat tgggtgccga tccggtgaaa gatattcagc attttaccgg
     2461 tcgcactgca ctgaaatttt tgcttgcggc cttgttaatc acacctctgg cacgctacgc
     2521 aaaacagccg ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc
     2581 gacactgcat ttaaccagtt acgcattact ggagctgggc gtgaataatc tggcgttatt
     2641 aggtaaggag ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt
     2701 gcttgcttta gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca
     2761 acagttgcat aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc
     2821 agtgaagatt atttcaccgc agccactcat ctacgctggg ctggcggtac tgcttttagc
     2881 cttacggtat aagaagttgc tttccctttt caaccggtta cgcaagcagg ttcacaacaa
     2941 attatctcta taacttgata ttgccgacaa agtacgcgtt atccctgccg gatgcggcgc
     3001 gagcgcctta tccggcatac acagatcgca ttaggttatg tttcgtttgt catcaacctc
     3061 aaatcatcga cataatttat tttatttcaa taagatagat aagcacacct gagagaacta
     3121 ttctcttgtc tcctaagagt tgttgcattt tgttatatgt tacaatataa cattacacat
     3181 catatacatt aactctggag gaaactgttt tggcgattcg tcttcataaa ctggctgttg
     3241 ctttaggtgt ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca
     3301 aacccttaac agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac
     3361 aaaaccgaac gctaagtgac tgggatggag tctggcaatc cgtttatcct ttattgcaaa
     3421 gtggcaaact tgaccccgtc tttcagaaga aagcggatgc agataaaact aaaacatttg
//
LOCUS       DAZGAP010000319         3467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGAP010000319 DAZGAP010000000
VERSION     DAZGAP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.321"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..575
                     /locus_tag="ACHLAM_005103"
     CDS             <1..575
                     /locus_tag="ACHLAM_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7856475.1"
                     /translation="SDAGGTGGKAMKLTKETGKSSWYLDHDAGTGAELLKNGGLISCR
                     FKVPGDLVANQYVMALYWPVSSLPQGVTLTGDAGNNLLASFYIQTDAKDLNVMYHNAK
                     VATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQVIPVIDGQDGAAFTLTQSPVGTFPVD
                     KLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            722..904
                     /locus_tag="ACHLAM_005104"
     CDS             722..904
                     /locus_tag="ACHLAM_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7856476.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            940..1185
                     /locus_tag="ACHLAM_005105"
     CDS             940..1185
                     /locus_tag="ACHLAM_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7856477.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENEPQPVEQPEPVQPGTDSAMM"
     gene            1260..1460
                     /locus_tag="ACHLAM_005106"
     CDS             1260..1460
                     /locus_tag="ACHLAM_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856478.1"
                     /translation="MDKNKKQPDTKGLEILSAEIINALRSGLDENTLQDRNFTTAMWI
                     AMQILCTPRCAIFAVREGQLTR"
     gene            complement(1704..2003)
                     /locus_tag="ACHLAM_005107"
                     /pseudo
     CDS             complement(1704..2003)
                     /locus_tag="ACHLAM_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2118..2303
                     /locus_tag="ACHLAM_005108"
     CDS             2118..2303
                     /locus_tag="ACHLAM_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7856479.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQETLLSV"
     gene            2272..3324
                     /locus_tag="ACHLAM_005109"
     CDS             2272..3324
                     /locus_tag="ACHLAM_005109"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO7856480.1"
                     /translation="MGNKKHYFQSDKSLTYGSVCSGIEAATVAWSNLGWKARWFSEIE
                     DFPSTLLSYRWPHIPNLGDMALLAPRIRDGLIEAPDILVGGTPCQAFSMSGKRKGLSD
                     PRGQLTLSFVELANQIDTVRASLNKSPSIIVWENVTGVLSSHDNAFGHFLGALAGAGC
                     ALQPARKRWANAGVVSGPSRIVAWRVLDAQYFGVAQARRRVFVVASAGKRLDPGKVLF
                     EFPPVSQGDKNYYGKKKKSESLLDQGIEGKLHRYCLEALPKRTGTVIAGYNGTTNQDM
                     TMSGGLIVERAPYSRVRRLTPTECERLQGFPDGYTDIPWKNSSPAHRYKAIGNSMPVP
                     VMKWIGRRIQEALNEV"
BASE COUNT         1021 a          738 c          828 g          880 t
ORIGIN      
        1 tcagtgatgc aggaggcaca ggtggtaagg caatgaaact gaccaaggaa acaggtaaat
       61 catcctggta tctggatcat gatgccggta ctggtgctga actgctgaaa aatggtggtt
      121 taatcagttg tcgttttaaa gtacctggcg acctggtggc aaaccagtat gtcatggcgc
      181 tttactggcc ggtttcctct ctgccgcagg gtgtcaccct gacaggggat gcagggaata
      241 atctgctggc atcgttctac atccagacag acgcaaaaga cctgaatgtg atgtaccaca
      301 atgcaaaagt ggcaacaaat aaccagaaac tgggaacctt tggtgcattt gataatgaat
      361 ggcatacgct ggccttccgt tttgcaggaa ataacagcct tcaggttatt ccggttattg
      421 atggtcagga tggtgccgcg tttaccctga cgcagtcacc agtaggaact ttcccggtgg
      481 acaaactccg cgtaacggat atcaccaaaa atgcaactta cccggtgctg attgacagca
      541 ttgtggtgga agtgaaaaaa gcggtaacag aataaaaaaa tcccgccggt catgatatgg
      601 ctggcgggag ctacctaacc cataaaggaa aataaaaata ctcccgaagg agtggttatc
      661 agtcgcttcg atgtttttac ccgataacga attatgtagt caatatcaaa ggtaaggtta
      721 tatgacattc gttcatacaa tgctgcttta tttctgcgct gtggtcagtg cgttatatct
      781 ggtgagcggc ggttataagg tcatcagaaa ttatattcgc cgtaaaattg atgatgcggc
      841 agctgaaaaa ctcagtaaaa cagcacaggc accgtcatcc ccaaacgacc cgaccccgct
      901 ctgataacgg ggcaatatac ctgcaaaagg agaatattca tgccagagat taaaggcacg
      961 gttactgaag agcttgtcaa acaggcactt tattctgaag aagtgaaccg cgtgctgaag
     1021 gcgcaggttc gcaaggattt tgaggcacaa atcgacgcat atgttgatga agtgctggcc
     1081 cgaatggttg gtcgttctcc ggctgaaaac agcacggaaa atgagcctca accagtagag
     1141 caaccagagc cggttcagcc cggaactgac agtgccatga tgtaacaata caaaccgaca
     1201 gcttctcctg tcggtttgat tttcatgggc gatgtatata ccagtcagag gaaataatca
     1261 tggacaagaa caaaaaacag cccgatacca aaggtctgga aatattaagc gcagaaatta
     1321 tcaatgcgct acgttcagga cttgatgaaa atacattaca ggatcgaaac ttcaccactg
     1381 caatgtggat agcgatgcag atcctgtgta ccccaagatg cgctattttt gcggttcgtg
     1441 agggtcagtt gactcgctga ttgcccgaat tacatcagaa aagcacgcgt tccataattg
     1501 catagctgat gaatcaccag ctgtatgaaa agaggtaaag acacaactgg tggatgtcat
     1561 tgctgcaatt gtgatatcag ctgcaattcg cgcagcctgt aacttttggt cggtggtcag
     1621 tttatccatt ttctcctgcc tgaacagatg catctctgct ttacaggaga ccggggaata
     1681 tcataggata caccacgagc tcagagtgaa gcattgatgc agttttttac agattgaccc
     1741 agaatagttc gcgctatctg agtcgccatt tatgtagtcc cgaagtaaag cattgatgca
     1801 aacttgtcta aatcggcccg gaatagttca cgctatctgg gtcgtccttt atgcatgtag
     1861 tatcccgttg atttgaaaca cactatacgt acttgcgatc ctaccttcac tggataaaaa
     1921 gtcaataaaa tgatcatttt atgatcgttt tcaccaccta actcttcgtt ttctttaagt
     1981 aaataatcac cgtcctgaat cattgttgat gacttttaaa tctatttgtt gtatcaatta
     2041 aacaaatcga atcgatcgtt tttgtcgatc gattgcatga gaaaggcatc aacaacaaag
     2101 caggaggtta tgcggccatg tatcgaaaca tacggtgccg acactgcaat aagctactgg
     2161 cacgggccag tttcagttat ctggaagtaa aatgtccgcg ctgtaaaacc ctcaatcaaa
     2221 tcacatctca gagcgccaca gagcacccca catacacaag gaaatcctgc cgtggggaac
     2281 aagaaacatt actttcagtc tgataagtca ctaacctatg gttcagtatg cagtggtatt
     2341 gaagctgcga cagtagcctg gagcaatctt ggctggaaag ccagatggtt cagtgaaata
     2401 gaggacttcc cgtcaacact tctttcatat cgctggcccc atataccaaa tcttggtgat
     2461 atggctttgc ttgcgcctcg tatcagggat gggcttattg aagcaccaga tattcttgta
     2521 ggtggaactc cgtgtcaggc attcagtatg tcaggcaaac gaaaaggcct ttcagatcca
     2581 cgggggcagt taacactgtc ttttgttgaa ctggcgaatc agatagatac cgttcgggca
     2641 tcgctcaata aatccccctc aataatcgta tgggaaaacg tcacaggagt tttaagtagc
     2701 catgacaatg cattcggtca ttttcttggt gcgctggccg gagcaggttg cgcattacag
     2761 ccagcaagga aaagatgggc aaacgctggt gttgtgtctg gaccatcgcg tatcgtcgcc
     2821 tggcgagtac ttgatgccca atatttcgga gtcgcccagg ctcgcagaag agtgtttgtt
     2881 gtggcaagtg ctggaaaaag gctcgatccc ggaaaggtat tatttgagtt cccgcctgtg
     2941 tcgcagggcg ataaaaacta ttatggaaag aagaaaaaat ccgaatccct tcttgatcaa
     3001 ggcattgaag gaaaattgca ccgatattgc cttgaagcat tgccaaaacg aacaggcaca
     3061 gtaatagctg gctataacgg aacaactaac caggacatga ctatgtctgg tgggctcata
     3121 gttgaaagag caccatattc aagggttcgt aggttgacgc caaccgaatg cgagaggcta
     3181 caaggttttc ccgatggata tacagatatt ccatggaaaa attcatctcc agctcatcgt
     3241 tataaggcca ttggaaattc aatgcctgta ccggtcatga aatggattgg tcgaagaata
     3301 caggaagcgt taaacgaagt ttaaacctaa gcaaaagaca ttatgaagtt ggtttttgtg
     3361 ccgttaaaag atgtctttaa ggagtgcaaa ggaaagcgcc aatttagtat caaaaaagat
     3421 gcttatcggc gcttttcatc ggtatcaaat gaaatgcaaa tcggtat
//
LOCUS       DAZGAP010000320         3401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGAP010000320 DAZGAP010000000
VERSION     DAZGAP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.322"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1096..2094)
                     /locus_tag="ACHLAM_005110"
     CDS             complement(1096..2094)
                     /locus_tag="ACHLAM_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO7856481.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNIETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFQRLKDRKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVVYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
     gene            2933..3127
                     /locus_tag="ACHLAM_005111"
     CDS             2933..3127
                     /locus_tag="ACHLAM_005111"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7856482.1"
                     /translation="MNQDKAIYLENKYFLVANEALRLERENNYLAAWIKWSEALYYAR
                     GKNISWCIARAEYCKKTGRF"
BASE COUNT          941 a          902 c          745 g          813 t
ORIGIN      
        1 gcctgattat tgagtttaat catcttattg ctgaacatgc cgttcatgat gaaaaactga
       61 ttaaccatac tgtctgtatt ctgaatgaat tacgaagcat agcgataaat taaaaaatcc
      121 ccctggatta cagggggaag ccatcagagt ttgagtaatg agaaatatta ttttggagag
      181 agcaccacaa atgcgctctt ttccaatctg ccgtaaccat cggcaaaaga caatcaggac
      241 aggcgaaaaa atccccctat atggcaaggg ggtgtcaatg aggacatggc aattgtgagt
      301 tttgtgtgtg ttttatgtgt tgcagtttgc aacgtcagaa atttgccgta gttcgtcatg
      361 agagacaaca aaaaaatccc ttggaactgg tggaggccaa gccaaggggt agcaaatggc
      421 taattaattc tcgtagtttt gatatttcca ttgttgcaac cgcaacgggg tgaatttgcc
      481 gcagttgtgc gcgaaaaaca atcctgattc cgggacagga tgtgtaatcg tataaccgcg
      541 cacgcacggc tatatgatta ccacctggtc agggctgcgc cccgacaccc tcgacaccgt
      601 tcccgcaata cgatagaaca tcagggcgcg tttccggtaa tcaactgacc atggggatgg
      661 tttgcattag caacaggtgc cggaaccgcg cacaaaatca tcagagacgc ggcgaacaaa
      721 aaacaaacaa aaacattgtc agctgcaaaa aatcgagtta aaacaaatgc gagcgccttc
      781 ctgtctcctt caattacggt cgtacattca ttgcatctgt cggggaggcg ctcacctcca
      841 taatccgatt tctccattga caataattcc ggtgataccc cctcttttga ggggcttttt
      901 ttacccacac accacaaaca gcagccatac catcgtcaaa aacgcgctca aaacgtctct
      961 gacgccctga accaaccaaa gcatacaaaa ccattgccca atgcaaaaaa gtgcgttaga
     1021 aagcgtctga gcgcgtttct ggtgagcaaa aacctgtcag ccgtggattt ccattgcaga
     1081 gacccagaaa cgcgctcaat catcgctgct tagccgacgt ttatctttcg cccgatcgcg
     1141 ctggtatttt ttgacaaccg gcacccacga aaacgccaga cgggaaccgt catctgtacc
     1201 ctctgccatg ccatccgcca tgattaaatc ctcgttcgtt tcccgctcta gttgcagaac
     1261 gttcttaagc gctccccccg tagcaacaaa acgacgcttg tgtacctgcc gtgttaattc
     1321 gaggaaccat tcaggatcag ccagcatatc agcaggcttt gtggaatatt tcagagtttc
     1381 aaccaccgcg ccgcgcagct gttccgcaac aacacaaggt aaagattcat cgtcttttgg
     1441 tttccgtgac ttcaccactc tgatatccac attcgggtca tacaccaccc gcagacaatc
     1501 acgccacagt tccacccaac gggcatgttt cacatactca cggccggaca acatcgaggg
     1561 gggaaccatc agcagagtgt gaaaatgggg atgtgcgcta ccatccttgc cgcgcgttac
     1621 ttccgtggta cgcacccatc ccagcacatg acgaaattcc ttgcgatctt tcagccgctg
     1681 aaaaccagca ttcatcgcgg tcagcgtctc accaagcgcg tcaatggaac agttgcgcac
     1741 cgtcagcgtg agaaacaacc agcgcgcgcc gggatgttcc gtcacaatct ttggcaggga
     1801 ctgatagaaa cgggcttgcc acatcatgga gcgcctccac tggcagatcg gacagtgccg
     1861 gacacgacag aaccgcgcat cacgcaaccg caggcgcgtt tctccggtct ctatgtttgt
     1921 tgaccaccca aaccgcaaaa taccactaca agccccgaca cgttcgccgt agtgctcaaa
     1981 tccggcagca cgcagataaa taccgcccac atcgtcggaa aagctccggc gctcatccca
     2041 cggtttatcc tccggggaat aatccgtcag atttttaagt tcaggatttt tcatgtctgc
     2101 cgttccctag tgagcgacag acgaaattac gtgttatcat tcctgtatca cctgtgttgt
     2161 cgtgtcacca attcgaattc ctacaggtgg tcacaccccc gaacgacgcc catcgttgcg
     2221 ggggtcgctt ttaatgcgac cacttccgac aaaatactct caaacctatt tccacgcaaa
     2281 tccgcgctca gcccagatgt gacaagggct agagctaaat tacaatccca gagatatccc
     2341 ttaagtataa atacaaggat cgcgcgcgcg atcgcccacc aaaaatgcgc gttttttgag
     2401 cgcttttccg cacaacaaaa caacacacaa aaaccactct cccgacaata ctccagtagg
     2461 ttttctcacc gattttccga acaacaaaac cgctttgcag gtggttggtc ccactgcgtg
     2521 acaccaacca ccatcaccag tgttttttct cgtaccgtcg tcaatcacaa aatcggaacc
     2581 cacgccggag gcgcgggacc ccacgatttt ctgtttgacg agtcagaaaa cggcgaacgc
     2641 tgacagattt caatctggct gcgcgagacg ttggcggggt gtcggggcgc agccctgacc
     2701 aggtggcatt tgtctgattg cgtgtgcgcg gtccgacaaa tacacatcct gtcccgtttt
     2761 tcgcacggtt tttattgatt accggacaga tgaacccgga ttgccatgtt gcgaaaatca
     2821 cgttaaaacg attacgagcg tgcagttggg ctgatatatc aacagtcatg ccaatatgta
     2881 ttaacccgaa ggccgctctt ttttaattca aataaaaata taaattacga gtatgaatca
     2941 ggacaaagca atttatttag aaaataaata ttttctggtc gcaaatgaag ctcttcggct
     3001 ggagcgtgaa aataattacc ttgctgcatg gataaaatgg agcgaggcgc tctattacgc
     3061 cagaggcaaa aatataagtt ggtgtatagc cagagctgaa tattgtaaaa agacaggccg
     3121 gttttaattc tgacggggtt tcaaaggggc gtcccctttg gcacgtcgac gtatcaaaat
     3181 gcgccagcat tttgtataca gtcggtaacg tgcctccagg attgccccat aatatttcct
     3241 atctacataa tgtctaacta agatccatct aatgtccatc taagatccat ttaaggtcca
     3301 tctaaatcag taaatatcta actagtgtcc atctaaggtc tatctaaagt ccatctaaag
     3361 gctaactaac gattcaccaa cggatcgcat gtagtagacg c
//
LOCUS       DAZGAP010000321         3396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGAP010000321 DAZGAP010000000
VERSION     DAZGAP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.323"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..594)
                     /gene="dsbA"
                     /locus_tag="ACHLAM_005112"
     CDS             complement(<1..594)
                     /gene="dsbA"
                     /locus_tag="ACHLAM_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO7856483.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYA"
     gene            complement(611..1597)
                     /gene="srkA"
                     /locus_tag="ACHLAM_005113"
     CDS             complement(611..1597)
                     /gene="srkA"
                     /locus_tag="ACHLAM_005113"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO7856484.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1674..1943)
                     /locus_tag="ACHLAM_005114"
     CDS             complement(1674..1943)
                     /locus_tag="ACHLAM_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO7856485.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2013..2597
                     /gene="mobA"
                     /locus_tag="ACHLAM_005115"
     CDS             2013..2597
                     /gene="mobA"
                     /locus_tag="ACHLAM_005115"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO7856486.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWIHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            2579..3106
                     /gene="mobB"
                     /locus_tag="ACHLAM_005116"
     CDS             2579..3106
                     /gene="mobB"
                     /locus_tag="ACHLAM_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO7856487.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(3209..3324)
                     /gene="rrf"
                     /locus_tag="ACHLAM_005117"
     rRNA            complement(3209..3324)
                     /gene="rrf"
                     /locus_tag="ACHLAM_005117"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          881 a          835 c          823 g          857 t
ORIGIN      
        1 agcatactgc tgaacaaaaa catccatatt gctggtatcc ataccctgcg gattcagctg
       61 atatttaccg ttaacaaaca tcgccggaac gccacgcaat tgcacgtcag ctgcagcttt
      121 ttcctgctga gcgaccaggg atttcaccac gaagctgttc cacgctgcgt cgtactcttc
      181 acctttaata cctgcgttga taaatacatc gcggatatca gatgctgaac gaatggtctg
      241 ggttttctgt acgccttcaa acagcggaac agtgactttg tcttccacgc ccagtgccat
      301 cgccactgcc catgcctgag tcagatcttt gcccaggtca ccccccatga agttgacgtg
      361 gtatttagtc atcttcacgc cttccggcag ttttttcttc acgttatcag aaatatgcag
      421 aacttcttca aactgatagc agtgcgggca gaagaaagag aaaaactcca gcacttgcgg
      481 cgcgccagca actggttttt ccagggtagt gtactgttta ccatcttcat actgcgccgc
      541 cgatgcgcta aacgctaaaa ctaaaccagc cagcgccagc caaatctttt tcatgatcta
      601 ctctctccga ttaatacata ggtgttaatt gcaaaggggg ttcttgtaga acttttgcct
      661 gttctataaa agtcgccgtc tgtcgcagcc agtaatcttc cccggttaac cacgggaaat
      721 ttttcgggaa cgcgggatca gcccaacgcc gcattagcca ggcaagataa taaaccaaac
      781 gcatggcgcg taaaggttca atcagtccga tttcagcggt gtcgaactcg ctaaattctt
      841 cataagcttc aataatagtt tccagttgca tccgctgctc ggctttatcg ccattgagca
      901 acatccacaa atcctgaata gccggaccat tacgtgcatc atccagatca acaaacattg
      961 gaccatcgcg ccagagaata ttcccggcgt ggcagtctcc atgtagccgc agaacggtga
     1021 aatcttcccg ccagtgtgct gtaacggcgg cgatcagctc atctgtcgct ttcagaaatg
     1081 ccgctttcaa cccggaaggt atcagtgtag cgtcctcaaa cagcttgcga ggctcaatga
     1141 gatattcatt cagaccgatg gtcgggcgat ggataaaaag ctgtttgcgc cccgtctggt
     1201 gcatacgccc taaataacgc ccaaccgctt ccatctgatc gatattatca gcttcgaact
     1261 ggcgaccacc gacgcttgga aaaacagcga aataaaatcc ctgatgattc aataaagtct
     1321 gaccgttaaa ggccacaggt gctgcgaccg gaacttcatc atttaccaac tgcaacgcaa
     1381 attgatgttc ttcgaggatt tgatcgggtg tccaacgttc aggacgataa aatttgacga
     1441 caaaacgtcg acgatcttcg tcctgaaatt gatagacacg gttttcatag ctgttaagcg
     1501 gggtaagacc ggaatccacc cggatccctt gctcaaacag agcatccatg atggtatccg
     1561 ggtgtagtgt ctggaaagta aaagcgctgt tgttcatccc atcatccgga aaacattacg
     1621 aatgatgaag gatatcattt cacgatgatt ttggtggcac cgcttacaag cttttactct
     1681 ttaattacgc cgcgagcgcg taacagcgcc gttttgaagt cttcttcata atctttctgc
     1741 aaacccggga tcaccgcatc tttcgcggaa tcacgcattt tcaggtgata gatcagaatg
     1801 tcatccgtca aatccgccag ttcgccgtca aaacctgact ctttcgccag tttctgcaaa
     1861 aattgcagca ggttaaggtc tggctctttt tgccaggctg gctggaggag ttcaataact
     1921 tcattcagac gtttacattt catagtaatg ctccttactc ttgagacaga cacgttagca
     1981 gggtcaatcc cacaataaaa gaggcgatat cggtgaatct gatgacgacg ataacaggcg
     2041 ttgtgctggc aggcggtaaa gccagacgaa tgggcggcgt agataaagga ttgcttgaat
     2101 taaacggcaa accattatgg caacatgtcg ctgacgcgct tatgacgcag ctctctcacg
     2161 tcgtggttaa tgctaatcgt catcaggaaa tctatcaggc aagcggtctg aaagtgattg
     2221 aagattcact ggcggattac ccaggccctc tggcaggaat gctttcagta atgcagcagg
     2281 aagcgggtga gtggtttttg ttttgtccgt gcgatacgcc ttacattccc catgatttag
     2341 ccgcccggct taatcatcag cgcaaagatg cgcctgtcgt gtggatccac gacggtgaac
     2401 gcgatcaccc gactattgct ctggtaaacc gcgcaattga gcctttatta ctggaatatc
     2461 tgcaagcagg agaacgccgg gttatggcat ttatgcgtct ggctggcggt catgcggttg
     2521 atttcagcga tcgtaaagag gcatttataa acgtgaatac gccagaggag cttgcccgat
     2581 ggcaggaaaa acgatgatac cgttactcgc ctttgccgcg tggagtggta ctggaaaaac
     2641 tacactgctg aaaaaactga tcccggtatt atgcgccaga gggatccgtc cgggactgat
     2701 taagcatacg caccatgata tggatgttga taaaccgggc aaagatagct atgagttgcg
     2761 caaggctggc gcggcacaaa ccatcgttgc cagccagcaa cgatgggcct tgatgacgga
     2821 aacaccagac gaagaagagc tggatctaca ttttctcgca agtcgaatgg atacctcaaa
     2881 gctggatttg attctggtcg aagggtttaa gcatgaagag atcgcaaaga ttgtgctgtt
     2941 tcgcgatgga gccggacatc gaccggaaga attagtgata gacagacatg ttattgctgt
     3001 agccagtgat gtgccgctta atcttgatgt cgcgttaatg gatattaatg atgttgaggg
     3061 gctggctgat tttgtggtgg agtggatgca aaagcaggac ggataagcaa aagtaggctg
     3121 gataagacgc gccagcgtcg catccggtat ttattctgct ccagcgcaaa aaggccatcc
     3181 tgacggatag ccttctgctt aatttgatgc ctggcagttc cctactctcg catggggaga
     3241 ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg
     3301 gaccaccgcg ctaaggccgc caggcaaatt ctgttttatc aacacgtctc gttatgacat
     3361 gtcgatttaa tctgtatcag gctgaaaatc ttctct
//
LOCUS       DAZGAP010000322         3364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGAP010000322 DAZGAP010000000
VERSION     DAZGAP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.324"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..1268
                     /locus_tag="ACHLAM_005118"
     CDS             24..1268
                     /locus_tag="ACHLAM_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO7856488.1"
                     /translation="MSKKFTKTLLSSSLVVGLLSVSSFTSASEYSYTALDIVGRHGTT
                     YTIVYNSDSDTAYVKWKTVPAGAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVY
                     DREFHDLMNSLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGVAASDGDVSYNVGVNFEF"
     gene            1355..1828
                     /locus_tag="ACHLAM_005119"
     CDS             1355..1828
                     /locus_tag="ACHLAM_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856489.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            2199..>3364
                     /locus_tag="ACHLAM_005120"
     CDS             2199..>3364
                     /locus_tag="ACHLAM_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO7856490.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTV"
BASE COUNT          870 a          634 c         1008 g          852 t
ORIGIN      
        1 gataatttaa caggatatcc ataatgagta aaaaatttac aaagacactt ctgtcatctt
       61 ctctggttgt aggtctgttg agtgttagtt cttttacaag tgcaagtgaa tatagctaca
      121 ctgccttgga tattgtcggt agacacggaa ctacttacac cattgtttat aatagtgatt
      181 ctgacaccgc ttatgttaaa tggaaaacag tacctgccgg tgctgaaggg attgaaagtg
      241 gaagtattgt acaaggatta tcaaaaatta atagagatgg gactgtagaa gttactacca
      301 aggatggcac tagtacatat cgtgtgtatg accgggagtt tcatgaccta atgaattcac
      361 tggtcaagca gaaaagagaa ggttctcttg gaaagaacga gggtactaaa acagagcttg
      421 tatctgatgc tgatattcgg aatctttttg gcagaattgc tgcgatctct gaaatggaaa
      481 ctaatgttgg tctggatgaa tatgggcgag tggcaactag tgcagagagt attaaagaac
      541 gcgtgtcttt gcagagttat ctggctacag aggctgcaaa tagcgcaatt ggcctagata
      601 aaaacggtaa gctaactctg aatgagacag atattgtaac gccgttacgt atcaatgacg
      661 gcgttgtcac tatagcgaaa gatgtggaag aacagaaaga agggctggcg atccagaaga
      721 aaggactgtc tgagctttca tcccgcacag atatcattga tgcagcaacg gctgcgaata
      781 cgaataaaat tgattcccga accaccgttg gtgtgaatgc ggacggaacc ttgaccagcg
      841 ctgatggcgc tacaaaaacg attgctgtta atgacggtct cgttgctctg tccggcagaa
      901 cagaccgtat tgatgctact gttggtagcg ttgaccgccg ggttactaaa aatacacagg
      961 ctattcagtc caatactcgc cagttgcagg aacataatgc acgcctgaac agccagcagc
     1021 gtcaaattcg tgagaatcac gaagagatga agcgtgcagc agcacagagt gcagcgctgg
     1081 cggggctttt ccagccgtac agtgtgggga aattcaacgc cactgctgcc ctgggggggt
     1141 acagtgataa acaggctgtt gccgtgggtg ttggttaccg tttcaatgag cagacagcag
     1201 cgaaagcagg tgtggcagcg agtgatggcg atgtgtccta caacgtaggt gtgaactttg
     1261 agttttaatt gctgacagtg acatgaagca tgacggcagg cgtggagttt tgcctgccgt
     1321 tttttctcat gtgagcatga aacaggtgac agcgatgaaa gtgattctgg cgacccgcaa
     1381 ccgttatctg gagtatggcc tgcagcagat gcttgagggg tacaggataa tactggcgag
     1441 ggagtttttt acaccagaga acaggaagag tgtaccggca catgatgagt catgggtgat
     1501 aatctgtgat gccctccttg gcaggctgat gtgctgcatg tttcagggac gccgttatct
     1561 tcagattgat gcggaagatg ttacggggcg cctggagacg tacaggaaaa tccggaatgg
     1621 tgagtgggta cataatacgt atgcgcgacc tctgacgatg tccgagatgg tggtgatgtt
     1681 tggatacgtg taccgggagt caaagccctg ccatcttgcc cgtgaaatgg ggataaacac
     1741 gaagacggtg aacacctttc tgtatatggg acttggtaaa aatggtctta agtacaggag
     1801 tgtgaagcac ctggttggac gtgcgtagcc ggaatgcata ttcgtgagtc gggtaatgag
     1861 agtggaacgg tggcagtgag aatggaacag aaaaaaatca gatgcggaaa gcggaaatac
     1921 acagcgggtg cgatgtgctt catgctgttt gtatggctgg ggggtgtggt gacattctgt
     1981 gtgctggccg ctctgataat gaggtgaaaa cggaagagag tttacttcct taattcgaac
     2041 tttctgataa ctgagaactt gttaaatgcc tgtgggttta tgtatttaaa tcaattggtt
     2101 aatggtgatt ttatctttat cagtcaaagt tcgttttagt atgatgattg tgcccgctgt
     2161 ttattgttgt ccgggagaaa atacagggaa tccaggggat gaacaaaata tactcactca
     2221 gagttaatga cagaaaagaa ctggttgctg taccggaaac ggtggggggc aggaaaaagt
     2281 cgtccggcat tcccggtgcg gggatgcttt cccgtcttct tctggcctca ggagccgttg
     2341 ccggcgttct gttttcttct cctgcgctgg catccgttgt cgggaatact ctgccatggc
     2401 agacctacag ggattttgct gaaaacaaag gggcgtttca tgccggggcc acaaatattc
     2461 ctctttatgg taagaatggg gctgtcagtg ggcggctgga caaggctccg atgatggatt
     2521 tcagtgttgt ggaccagata ctgggggtgg caacactgat ttctccgcaa tatgtggcgg
     2581 gggttaagca taacggcagc tacaatacgg tgcgttttgg ttatgcggac gatacgacat
     2641 atcgccttgt tgaccgtaat gagcactggc gtgattttca tactccccgg ctgaacaaac
     2701 tggtgacaga agttgccccg gtgagcgtca cggatgccgg aaccggcaag ggggtatacc
     2761 agaaccgcag tcgttatccg gtgttttatc gtatggggag tggaacgcag tacaccggag
     2821 ctgcttccgg tgcgctgacc agaatagcgg atgcttacgt ctggaggact ggcgggacgg
     2881 tgggaagtcc ggtcatttct gactggagtc tggtgtcaaa cccgggaaat ctttaccagg
     2941 gtgttaatgg tccgctggcg tcttacggga catcaggaga cagtggctca ccgctttttg
     3001 cctgggatgc ggtgaagaag cagtgggtat ttgttgctgt acttcatggt tatgccggtg
     3061 agaacgggaa gacgaactgg tttacggtga ttcctgcagg ggatgtgaat aacacgataa
     3121 agcaggacag ttcaggcacc gtcgtgccgg cagttgccgg tggtgacatt gtgtggaatt
     3181 acagtaaggg aagcggagag ggaacccttt ctcaggatgg gaaggtatgg aagatgaacg
     3241 gcttcagggg agggagtctg aatgacggga aagatatcac ctttgggggt aagggcacgg
     3301 ttgttctgaa ggatgatgtt gtccaggggg cgggttcgct cacgtttaat ggtgattata
     3361 cggt
//
LOCUS       DAZGAP010000323         3331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGAP010000323 DAZGAP010000000
VERSION     DAZGAP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.325"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1012..2028)
                     /locus_tag="ACHLAM_005121"
     CDS             complement(1012..2028)
                     /locus_tag="ACHLAM_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO7856491.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLAERPAARWLFMTLTVKNPPVEDTRETLIRMNAAWNRLVRRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLIGADIPAELDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
     gene            2863..3057
                     /locus_tag="ACHLAM_005122"
     CDS             2863..3057
                     /locus_tag="ACHLAM_005122"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7856492.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIDWCIARAEYCKKTGRF"
BASE COUNT          925 a          876 c          714 g          816 t
ORIGIN      
        1 gcctgattat tgagtttaat catcttattg ctgaacatgc cgttcatgat gaaaaactga
       61 ttaaccatac tgtctgtatt ctgaatgaat tacgaagcat agcgataaat taaaaaatcc
      121 ccctggatta cagggggaag ccatcagagt ttgagtaatg agaaatatta ttttggagag
      181 agcaccacaa atgcgctctt ttccaatctg ccgtaaccat cggtaaaaga caatcaggac
      241 aggcgaaaaa atccccctat atggcaaggg ggtgtcaatg aggacatggc aattgtgagt
      301 tttgtgtgtt ttatgtgttg cagtttgcaa cgtcagaaat ttgccgtagt tatacgtgaa
      361 aaacaatcct gatttctgga caggatgtgt aatcgtataa ccgcgcacgc acgccgatac
      421 gattaccacc tggtcaggtc tttgcccgac accctcgaca ccgttcccgc gatacgatag
      481 aacatcaggg cgcgtttccg ggtaaacgca tgttaatgac cgggaattca ttgattaggg
      541 accggagacg cgctacaaaa ctcctgcgcg tttctggcat caactgacca tggggatggt
      601 ttggcagtag cagcaagcgc cagaaacgcg ctcaaaatca tcagatgtac agcgaacaaa
      661 aaattgtcat cggcaaaaaa tcacgttaaa acaatcgcga gcaccttcct gtctccttca
      721 attacggtca aacattttgc atctgtcggg aagttgctca cctccataat tcaatttctc
      781 attgacaata attccggtga tatcccctct tttgaggggc tttttttacc cacacaccac
      841 aaacagcagc cataccatcg tcaaaaacgc gctcaaaacg tctctgacgt cctgaaccaa
      901 ccaaaacata caaaaccatt gcccaatgca aaaaagtgcg ttagaaagcg tctgagcgcg
      961 tttctgacgt cttttttgtt gctgtgtcgc tactcgttgt cagattcgcg ctcaatcctt
     1021 catcctgtca gcttcaggat tccgcttgta acgtttttcc gtacgtcccc agtcaaacgc
     1081 cagccagaag gcttgctcct cgaactcgtc cagttctgcc ggaatatctg ccccgatcaa
     1141 atcctcgtcc gtctccggtt tatcgacttt cagaacctct ttgagacgac cgccggttgc
     1201 caccatgcga agcccatagg tctgccgcgc cagctccaga aaccacgccg gatcacgcac
     1261 aacttccgaa gatttcaccg tgtatttcat cgtctcaatg acgcctgact ccagcgccgc
     1321 acgatgcata tcaggcaggg ttacaccatc cggccgtttc cagccctgtt tcggttttac
     1381 cacccgaata tccacacccg cctcataatc gacacgaagg cattcactcc agatctccac
     1441 ccagcgagca tgtttcacat aaccattacc ggacaacatc gaagggggga ccatcatcag
     1501 aacgtgaaaa tgcggatggc aacatcctgg caccttcccg taagtaattt ccgtcgtacg
     1561 taaccagcca agaactgacc tgaattcttt acgccgaacc aacctgttcc acgccgcatt
     1621 catccgaatg agtgtttcgc gggtatcttc cacaggggga tttttgacag tcagtgtcat
     1681 gaacaaccag cgggccgcag gacgttcggc cagcagcttt ggcaaggcct cccagaggcg
     1741 acgcatccag cggagagagc gacgacgact acacaccgga caaaacggaa catgacaaaa
     1801 ctcccccttc cgaagggcca gcttcatttc gcccgtcagg cgggacacct tcggcgcaaa
     1861 caacaaccga cgggaacaat caaacatccg ttctccacga gaggaaaaca tcgaatcgct
     1921 ttcgtaaatc tgcgccaccc tgtcagccat cactcgatca ctgtcccatt ttgtatcccg
     1981 cgtgctgtaa tcgcgtaacg agagtaccgg actgtctgga tttttcattt gcacccccca
     2041 agaactgggc agatgcggaa attttgtgtt agcatttttc tgtcacctgt gactctgttg
     2101 ccgaacaacg aattccaaac tggtgatcgt cccctggagt gttaccgcac tcgcagggga
     2161 accccctcaa aatcgttcat tttttaacca gaagttttta tctcttttcc acccattttt
     2221 accgctcaaa gccgcgccac cactgggcta agccaaaata acacatcctt gagttgttgc
     2281 ctatagtata aatacaagga tcgcgcgcgc gatcgcccac ccaaaatgcg cgttttttga
     2341 gcgttcccga gaacaaaaaa acaacacatc caaaccacct gatcagcagg tttctcaccg
     2401 actttctgaa caacaaaacc gctttgcagg tggttggtcc cactgcgtga caccaaccac
     2461 catcaccagt gttttttctc gtaccgtcgt caatcacaaa atcggaaccc acgccggagg
     2521 cgcgggaccc cacgattttc tgtttgacga gtcagaaaac ggcgaacgct gacagatttc
     2581 aatctggctg cgtgagacgt tggcggggtg tcggggcgca gccctgacca ggcggcattt
     2641 gtctgattgc gtgtgcgcgg tccgacaaat acacatcctg tcccgttttt cgcacggttt
     2701 ttattgatta ccggacagat gaacccggat tgccatgttg cgaaaatcac gttaaaacga
     2761 ttacgagcgt gcagttgggc tgatatatca acagtcatgc caatatgtat taacccgaca
     2821 ggccgctctt ttttaattca aataaaaata taaattacga gtatgaatca ggacaaagca
     2881 atttatttag aaaataaata tttttcaccc gcaaacgaag ctgtcagact ggagcgtgaa
     2941 ggcgattaca ttggagcatg gaaaaaatgg aatgatgcgc ttcattatgc caaaggagaa
     3001 aatatagact ggtgtatagc cagagctgaa tattgtaaaa agacaggtcg gttttaattc
     3061 tgacggggtt tcaaaggggc gtcccctttg gcacgtcgac gtatcaaaat gcgccagcat
     3121 tttgtataca gtcggtcacg tgcctccagg atttaccatc aaaaacgccc ctgtagttaa
     3181 tcctgaatca agatataact aagatataat tagcgtataa ctaaggcata actaaagtat
     3241 aactaactta gtgtgcatat aattaagata taactaatat ataactaatg tataactaac
     3301 ggttcaccaa cggatcgcat gtagtagacg c
//
LOCUS       DAZGAP010000324         3315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGAP010000324 DAZGAP010000000
VERSION     DAZGAP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.326"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(83..832)
                     /locus_tag="ACHLAM_005123"
     CDS             complement(83..832)
                     /locus_tag="ACHLAM_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO7856493.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEIILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(922..1095)
                     /gene="yciZ"
                     /locus_tag="ACHLAM_005124"
     CDS             complement(922..1095)
                     /gene="yciZ"
                     /locus_tag="ACHLAM_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO7856494.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            1133..1261
                     /gene="ymiD"
                     /locus_tag="ACHLAM_005125"
     CDS             1133..1261
                     /gene="ymiD"
                     /locus_tag="ACHLAM_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO7856495.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIITEYIAKYIYSSTYARFR"
     gene            complement(1243..3228)
                     /gene="pdeR"
                     /locus_tag="ACHLAM_005126"
     CDS             complement(1243..3228)
                     /gene="pdeR"
                     /locus_tag="ACHLAM_005126"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7856496.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT          894 a          800 c          768 g          853 t
ORIGIN      
        1 aatagtctta attatcaacc aggaatcatc ttagagcgga tgatttgcca aagtgcaaat
       61 catccgtaga gaagggaaat ggttaaatgt caacaacgtg aacggtgagt ttactgtgct
      121 caagatgcat cagatcgctg gcgcgtattt tcgaatcggt aatcacgcga ttgaatcgct
      181 caacgggacc gatggagtat gaatgtacag caccaaattt cgagctgtca gtcaggacta
      241 tcgcttcgca ctctttttcc agcacggcat tgaccacatc ggtacgcatc atatcgcgac
      301 cggtgaatcc agtttcaggt tgccagccat caataccaat aaatgctttg ctgaaatgta
      361 cctgttggat acactggcgt gtcaacgggc caaccatact ttcgcttttt ttctggtaca
      421 cgccaccgag caaaataatt tcacaaggcg cgtctttcag caaatgcgcg atgtagctgc
      481 tgaccgtgat gatagtgaca tttttcttct gctcgcccag agtccgggcg agcagggcat
      541 tgctgctgcc attttcgata aagatggttt cgcccggttg aaccagtgac gcggcaaact
      601 cggcgagttc acgcttcagc gtatagttgc tcatcatacg ggtttcgacg tcatcactat
      661 caagcgatac tgcaaagcca tgtgcacggc ggaggtaact cagtttttcg agggtgttga
      721 gatcctggcg aatggtaact tcagaaactc cagtggcttt tgccagatcg gttacgctaa
      781 cctgaccctg gtcaatgacc atctgtagaa tagtttgttg tcgggaattc atcgctgtaa
      841 ttcttataac gttataatac taaataaaaa aatactgacg cgccgggcat cctcaaatag
      901 gaaaacctga aggcacgatt gttaaatctc ccacggtgcc ttcgggatgt ctggcgtaga
      961 ttcagcaacc tggcgcaaga gttctgcttt aagtatctcg agattatgga tcgcagagtg
     1021 gtaatcgcct gccacaagga tatctaacac aatatcaata cgttcggcaa cgcgctgggc
     1081 atcaaattca gacatcaagg acatccttat agcaaaagtg ggaaaatgaa taatgcaaaa
     1141 attgccgcta aaagagaagt gtttaacagc aacggctaat tatcatccgg gaatacgata
     1201 tataattacg gaatatatcg ctaagtatat atattcatct acttatgcgc gcttcagata
     1261 gcgtttatac caacgttcga aggcgacggc gggcatcggt ttggcaaaca aaaatccttg
     1321 ccgctcattg atcccgttct tggttaaaaa agcatcttcc tttgcactct ctacaccttc
     1381 ggcgatcacc tgaagattca atgcctgggc cacagcgacg atcgcccgga ccagtgactg
     1441 cgagacaggt tgtttgtgaa tatctcgaac aaaaacctgg tcaagtttga tggcatcgat
     1501 cggaaagcgc gccagttgcg aaagtgaaga gtaaccggta ccaaaatcgt ccagatgcac
     1561 ttgcgcacct agttggctaa attgttgaat aacagacagt gccagctcat cattctcaat
     1621 caggcagctc tccgtcagtt caacatcaat agggcagtat tcaaaattga gttcctggag
     1681 aacctgtttc agggcggtga aaatggtttg atcggcgagc tgacgtgcag aaatgtttac
     1741 cgccactcgc aggttaatac ctttatcccg ccactttgcc acctggcgta ctacatcgag
     1801 aatcacccaa cggcctaaag gcacaattag ccctgactct tcggcgtagg aaatgaagtc
     1861 caacggtgga atcaatccac gttcaggtga ctgccaacgt actagtgctt ccagactgcg
     1921 cacttcgcca cgccaggtga ttttcggttg atagtgaata accaactgat cgttttccag
     1981 tgctttacgc aagttggtat ccagccagag atattcaaat acccgctgat tcatttctgg
     2041 agtaaaaacg caaaattgtc ctcgtccgcc ttccttcgct gtgtacattg ctgtgtcggc
     2101 gtgacgaata atagccgcgc tgtccgaacc atgttcggga gagagtgaaa tacccactga
     2161 acagctggta taaacttcaa ttaaaccaat gcggaagggg agtcgtaagc gggtcaaaat
     2221 tcgtgatgcc attgcttcca gcgcgctttg tgaggtattg gatgccagta ccagaaactc
     2281 atcgccacct ggacgcgcca acacctgatc atgttcgaga cagcttaaaa tggccaatga
     2341 cacgtcgcgt aatagctggt caccaaacaa atgcccataa gcgtcgttga cctttttaaa
     2401 attatccaaa tcaagataca caaccccaac tttattgtta tctgcctgat taatggcgtg
     2461 atcgattaac tcctgcattg cgttacgatt cggcagtccg gtgatactgt cggtatttgc
     2521 cagaatacgc agtcgctcct gagcgcggcg ctcttcggta atgtcggtgc cggaacagat
     2581 taaaaaaatt tcgttcttgc cactgccgct gtggacaaat ttattgcgaa acagaaacag
     2641 ccgctggcct ttacgtgttg gtatccacag ttcgacttca tatgcattgc cgcttcgaaa
     2701 aaatacacgg ttattgcgcc tggatgccgc agcttcacga cggctcataa acagtttaaa
     2761 cacgctttgc ccaatgacgt catgttcttt caaccctgtg taatcttcac ataaccgatt
     2821 gaagcgttgg atattccccc ggctgtcgag aatcactatt gcggaatgtg cttcagaaac
     2881 tacctgctca gcaaaggaaa gtccgtgtga taaatcacga gcaactgctg gtgtgtcatg
     2941 ccatgcagac gcgttgccag cccattcccg tttattgatt tttcgtccta ccagatgcac
     3001 agaaagatcg ctttcatcga cggtcagact catcatcagg ctggaggtga tgaccgtcat
     3061 ttccctgatg cgagcagcct gttcggcaga taactgcaac gtacgttcag gttctgtggt
     3121 ttcggtggta gaaaagcgca aaacatcgct gctttccgtt aaccgccagt atggattgtg
     3181 cgatccgaga aagttgtaca acgttgtgga ctccctaacg gttttcatga tgcatatcct
     3241 ccggttaaca gcggagtccg cggttaaata aaaggaacaa catgacttac atgaaattaa
     3301 cggcggctaa acgca
//
LOCUS       DAZGAP010000325         3288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGAP010000325 DAZGAP010000000
VERSION     DAZGAP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.327"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            255..773
                     /locus_tag="ACHLAM_005127"
     CDS             255..773
                     /locus_tag="ACHLAM_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7856497.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            983..3091
                     /locus_tag="ACHLAM_005128"
     CDS             983..3091
                     /locus_tag="ACHLAM_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7856498.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQVIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          834 a          816 c          936 g          702 t
ORIGIN      
        1 cattttatgg aggcaatttg gactttgaac agttgggtat ggttatccag atatatatta
       61 ttaacttatt gctgaataag agatttaatg attttaaaaa tagtttttac gacaaccgta
      121 aattctagct cggtttgtat gtaaaaaata ctgaaaaatg atgcggttcc gtgaagagag
      181 ctgcatctgc ctgtctggga ttaacggcag gtcattgtaa gtagtaatta accacgttaa
      241 caaggagttt acaaatgcca acaccgtgtt atatctccat cactggtcag acccagggaa
      301 atattactgc aggtgcattt accgctgatt ctgtcggcaa catctatgtg caggggcacg
      361 aagacgagat gctggtgcag gaattcctgc ataacgttac tgtcccgacc gatcctcagt
      421 ccggccagcc tgcgggtcag cgtgctcaca agccgttcat ttttaccgtg gcgctgaaca
      481 aagctgttcc gctgatgtac aacgcactgg cctccggcga aatgctgcca accactgaac
      541 ttcactggtg gcgtacctct gtggaaggca aacaggagca ttatttcacc acccgcctga
      601 ccgattcgac gattgtcgat atgaaactgc atatgccgca ttgccaggat ccagcgaaac
      661 gcgagttcac ccagttgctc gaagtttcac tggcctaccg caaaatagag tgggaacacg
      721 tcaagtctgg cacctccggt gccgacgact ggcgcgcacc gctggaagca taagttaagc
      781 caacagcatc cggctatcag gccggatgct gtttttgtat tacgccgtaa aaagaaaggt
      841 tgtcagtaag ggattacgtt cacgggagat ggcggatgcc ggaaacttcg gcggtagcgt
      901 gcctgaacgc aagcgtaaca ctgtactgtt acagccgctt taacagcgaa ctgtctgttt
      961 ttccttaaac ccggaggttg ctatgtcaac cggattacgt ttcacgctgg aagtggacgg
     1021 tctgccaccg gacgcgtttg cggtggtctc ctttcatctg aaccagtcac tctcttcgct
     1081 cttttccctc gatctctctc tggtcagcca gcagtttctc tcccttgaat ttgcgcaggt
     1141 gctggacaaa atggcctacc tgacggtatg gcagggcgat gacgtacagc gccgggtgaa
     1201 aggtgtggtg acctggtttg aactggggga gaacgacaaa aaccagatgc tgtacagcat
     1261 gaaggtgtgc ccaccgctgt ggcgcacagg gctgcgccag aacttccgta tcttccagaa
     1321 tgaggacatc gaaagcatac tcgcgacgat cctgaaagaa aacggggtga ccgagtggag
     1381 tccgctgttc agcgagccgc atccttcccg tgagttttgt gtccagtacg gcgaaaccga
     1441 ttacgatttc ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg aggagcacgc
     1501 gcaaaaaagt accgaccaga gcctggtcct gtgcgacacc gtgcgttatc tgccggagtc
     1561 ctttgagatc ccctggaatc cgaacacccg taccgaggtg agcaccctat gcatcagcca
     1621 gttccgctac agcgcacaaa tccgcccatc ttccgtggtg accaaagact acacctttaa
     1681 acgacccggc tgggcagggc gttttgatca ggaaggccag caccaggatt accagcgcac
     1741 acagtatgaa gtgtatgact accccggacg tttcaagggt gcccacgggc agaactttgc
     1801 ccgctggcag atggatggct ggcgaaacaa cgcagaagtg gcgcgcggaa caagccgttc
     1861 gccggagata tggccgggac ggcgaattgc gctgacgggg catccgcagg cgaacctgaa
     1921 ccgggaatgg caggtggtgg caagtgaact gcacggcgag cagccgcagg cggtgccggg
     1981 acgcaggggt tcaggtacca cgctgaataa ccactttgcg gtaataccgg cagacagaac
     2041 atggcggcca cagccgttgc tgaaaccgct ggtggacggc ccgcagagcg ccgtcgtgac
     2101 gggaccggca ggagaagaaa tcttctgtga tgaacatggc cgcgtgcggg tgaaatttaa
     2161 ctgggaccgc tataacccgt caaaccagga cagttcatgc tggatccgtg tggcacaggc
     2221 gtgggcaggc accggctttg gcaacctggc gataccgcgt gtgggtcagg aagtcattgt
     2281 ggacttcctc aacggcgatc cggaccagcc gattattatg gggcgtacct accaccagga
     2341 aaaccgcacc cccggcagcc tgccgggaac aaagacgcag atgaccattc gttcgaaaac
     2401 ctataagggc agcgggttta atgaactgaa gtttgacgat gcgacaggga aagaacaggt
     2461 ctacatccac gcgcaaaaga acatgaacac cgaggtgctg aataaccgga ccacggatgt
     2521 gataaacaac catgctgaga ccatcggcaa caatcaggtg attgcggtta ccaacaatca
     2581 gatacagacg gtgggcgtta accagataga gacggtgggc agtaaccaga tcattaaagt
     2641 gggctctgtt caggtggaaa cgattggact tgttcgtgcg ctgaccgtgg gcgtggcgta
     2701 tcagacgacg gtaggtggca ttatgaacac ctcggtggcc ctgatgcagt cctcgcagat
     2761 cggtttgcat aaatcgttga tggtcgggct gggttatgac gtcaaagtcg gaaataacgt
     2821 taccttcacc gttggtaaaa cgaaaaagga tgataccgga cagaccgcga tttactccgc
     2881 cggtgagcat ctggaactct gctgtggtaa ggcaaggctg gtgctgacta aggacggaca
     2941 aatttttctc aacggcacaa aaattcattt gcagggtaag gagcaggtta atggtgactc
     3001 actgttgatt aactggaact gtgcagcctc gaaatcccca ccgaagaccc ctgatgaaaa
     3061 gcaggatacg ccggatatga gagagtactg atcccgtacc tgttcggtat tcagtaacaa
     3121 acaagcatta aaaataaaca gaagaaggaa ttgaatcatg agcggaaaac cagcggcgcg
     3181 tcagggagat atgactcagt atggcggtcc cattgtccag ggttcggcag gtgtaagaat
     3241 tggcgcgccc accggcgtgg cgtgctcggt gtgtccgggc gggatgac
//
LOCUS       DAZGAP010000326         3275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGAP010000326 DAZGAP010000000
VERSION     DAZGAP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.328"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..579
                     /locus_tag="ACHLAM_005129"
     CDS             88..579
                     /locus_tag="ACHLAM_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856499.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     ASSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            582..1850
                     /locus_tag="ACHLAM_005130"
     CDS             582..1850
                     /locus_tag="ACHLAM_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFO7856500.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMAVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGFILIPGK
                     GWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRASI"
     gene            complement(1993..3270)
                     /locus_tag="ACHLAM_005131"
     CDS             complement(1993..3270)
                     /locus_tag="ACHLAM_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7856501.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWMLIKVKDEEPQVQLATS"
BASE COUNT          946 a          671 c          783 g          875 t
ORIGIN      
        1 aatcttgaac gctttgcagg tgcgttcaat ccaataaatg ctaatattat ggctatcata
       61 gccataaaaa gataaaatat tatcactatg ggtaaaaaaa cagcaccgtt actcccttat
      121 acgaatcaga tgcttagtga atttggtgag cggctgaaac ttgctcgttt acgacgcagg
      181 cttaccgcaa agcaagttgc tgaacgtgct gggatgtcgc caataacatt gcgctctctg
      241 gagtcaggaa gtgcaggggt cacgataggg gcatatttgt ctgtgatgca ggttttgggc
      301 ctggaaacgg atttaaataa gcttgcagct gaagacgact tagggcgtca gctccaggat
      361 tcccgtttga ttaaaaaaag agggctttct cgtgcatcaa gtaagaatga agaaggttct
      421 cagacagcct cattgcagat gaggcacgag aaagaaggtc atctcaccac ggatttaaca
      481 gcactggcag aaggctcgac gccatctatt acaggagaaa catcaacctc tcttgcagca
      541 ctattaaaac cgataaaaaa accgttaaaa ggaaattaat tatggcatta atcggagtgt
      601 atgcggattg ggaaggcctg gatggtccgg agcggattgg ttatttacac agtcgccgca
      661 ctcggacgcg tgagatattt gagtttgagt acgataaaaa ggcgcttgca gatccttcac
      721 ttaatttcat tcagttagat cctgaaataa tgttgtatga gggagcacag tacccaattc
      781 ctccaaagga taaatttggt gcatttagtg attcctgccc ggatcgttgg gggcgtatgc
      841 taatgaagcg tcgttttgag agggatattc gagacgggtt atgtgataaa gactcccacc
      901 tttatgagtc tgattatcta ctaggtgttc atgatcttta tcgggttggg gcccttcgat
      961 acaaacgtga agacgctgga gagtttcttg ataatcgcat tgatgttgca gccccaccat
     1021 ttaccgaaat agcgagtctg gaaagggcta gccgggcaat tgaagaagat cctgataata
     1081 aagagctaat ggggcaaaaa tggttaagaa tgctaatcgc tccgggggga tcattagggg
     1141 gggcacgtcc gaaagccagt gttgttgatg aagctgggca tctctacatc gctaaatttc
     1201 ccagtgtaaa agatgagtat gatgttggtg gatgggaaat ggccgttaat gcacttgctg
     1261 ttggctgtgg actgaatgtt gcacctgctc aggcgcataa atttgccagc aattaccatt
     1321 gttttatggt tcgtcgtttt gaccgcacta atgcgggaag gcgtctgcat tttgcttcgg
     1381 ctatgacact aaccaggcat caggatggtg aagatgcctc tacaggtgtg agttatctgg
     1441 aattagcaga tgttcttatc aggcatggcg cgcagactaa tactgatctt aaggaactct
     1501 ggtccaggat tgtttttaat attcttgttt ctaataccga tgatcattta cgcaatcatg
     1561 gatttatctt gataccagga aaaggttggc ggctttctga ggcatatgat ttgaatcctg
     1621 tggtcaggtc tgatggttta aaactcaata ttacggaaaa tgacaatgca cttgatttgg
     1681 aattagctag agaagttgca gagtattttc gacttagttt gacggaagct gatgacatta
     1741 ttgctaattt tagagggatt gtcagtcagt ggcgaatcat tgccgagcgt ctgagattac
     1801 ctggtcgtga gcaagagtta atggcagagg cattcagagc gtcaatttag aatagttatt
     1861 tcgatatatt cgctaaatca taaatttgcg aagcaatgcc agtagatact gaacgttttg
     1921 gaagagacgt ttaatttatg agaaaaatag aaacgctaca aagctaaatt atagcgtttc
     1981 tatgtaaaat aattagcttg tcgctaactg tacttgtggt tcttcgtctt taactttgat
     2041 aagcatccac agcgcaatgg cgccaataat gtcaaagaaa gccagggcaa taaagaacgg
     2101 cgcgaagcca ataatgtgaa cgaatgcccc gataaacagg ttgaaaatca actgaccgct
     2161 ccatgcacag gctcctgcca tacccacagc ggtagctact tcgtcttttt tgaataggtc
     2221 accgcccaga gttgctgcaa cagtagaaag acattgatgc gagaatgcgc caatgctaat
     2281 cagtaatacg gcgatgtagg gattttcgac aatactcacc agaccaatcg tcatcataat
     2341 gacggctgca atagtgaagg taattcttcg tgagttaatt aaactcacgc cgcgatcgtg
     2401 gaagaacttc gcgacaaaac cactggctaa acagccgaga tcgcccagca ggaaaggtag
     2461 ccagacgaac atggcaattt ctttcaggct aaaatgcagc gtttcgacga agaaaatcgg
     2521 tacccagaag ttaatggttc cccatgccgg gtcagcgaga aaacgcgcga tgccaatccc
     2581 ccagaaatta cgttggctga ggatatgctt gattgacgtt ttttctttct tatctgtttt
     2641 aagatgctgt tcttgcccag actcaatata gtggcgctct tcatcagaaa ggcgtttagc
     2701 atcttttgga tctttataac aaaagaacca aaataaagcc gcgagtaaag caaggctacc
     2761 tgagacaata aacgcaaatt gccagctatg aaacataatg caccaggcaa tcaacggtgg
     2821 agcaagcatc gcgccgagtg aggtgcccat attgaaaaca cctgtcgcca cgccacgctc
     2881 ttttgccggg aaccatgttg atgcggtttt tacacccgca ggaattgccg atgcttcgct
     2941 gaaacccatc agggcgcgca taaatgccag acctgaccat gttcctgtca aggcatgccc
     3001 cattgtcgcc aggccccaga ttgcggcaca tatcgcaaaa cttaacttca gacccacggt
     3061 atcaataaag aagcccatta tcggttgccc caatgtatag gccaactgga atgcactgac
     3121 aatccatgaa tattgttctt tggttattcc ggttgtctcc ataatcgttg gagccgcaat
     3181 acctaacgcc gttctgtcca gataattgat tacagtgata aagcatacga gggcaatcat
     3241 atgccatcga taattcttta ttttattcat tttta
//
LOCUS       DAZGAP010000327         3261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGAP010000327 DAZGAP010000000
VERSION     DAZGAP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.329"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..1634
                     /locus_tag="ACHLAM_005132"
     CDS             84..1634
                     /locus_tag="ACHLAM_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO7856502.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANISGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1675..>3261
                     /locus_tag="ACHLAM_005133"
     CDS             1675..>3261
                     /locus_tag="ACHLAM_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO7856503.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLANVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPKKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKITPAQ"
BASE COUNT         1015 a          683 c          696 g          867 t
ORIGIN      
        1 actatgctta cataaaataa gacacgatgt gtcttttatt taaataattc aacaaatgta
       61 acacacccta tccaggaggg atgatgaaaa ctgtatttac aacattatca gtcatattcg
      121 gcgtgatatt ttctcacagt gttctagctc aggacgttac tatttattat acgaatgaca
      181 ttcacgcaca tgtcaatcca gcaaaaatca tcgctgttga taagaacaga cttataggtg
      241 gtatggctaa tatttcaggc atcgttaatg aagcgaagaa aaaaagcaaa gatgtgtttt
      301 tctttgatgc aggcgactat tttaccgggc cgtatatcag caccctgacc aaaggtgagg
      361 ctattattga tatcatgaac actatgccct ttgatgcggt gtctgttggt aatcacgagt
      421 tcgatcacgg tgtgcccaat atggtgtctc agttatcaaa agcaaaattc cccattctgc
      481 tgggaaatat ctactacacc gatacgaata aaccagtttg ggatcatccg tggaccatta
      541 tcgaaaaaga tggtttaaag attggtgtga ttggattgca tggcgcattc gctttctatg
      601 atacagtcgc ggcaaaagca cgtgaaggcg tagaagccag agacgaaatt aaatatttaa
      661 ataaagcact tgcagaatta aaaggtaaag tcgacattac cgttttgcta attcacgaag
      721 gtgtcccggc gcgtcaatca agctttggta gtaaagatgt ggagcggcta ctacaggcag
      781 atattgagac agctaaaaaa gtgaatggtg ttgatgtctt aattaccggt cacgcacatg
      841 tcggaacacc gcaaccaata aaagttaata acacattaat tgtatctacc gatgcatacg
      901 gcaccaacat cggaaaactt gtgcttgatt acaatccgaa aacgaagaaa attgacagtt
      961 ataatggtga gttaatcacc atctttgcag atcaatttaa acctgacacc atcgttcaaa
     1021 atactattga taaatggagt gcaaagctta acaaaataac acaggaagtt gtcggccatt
     1081 ctcccgtagt tttaacacgt gagtatggta gttcctcttc taccggcaac cttattcttg
     1141 atgcaatgat ggaaaaaaca cctgatgccg ttgccggatt tcaaaatagc ggtgggattc
     1201 gagctgattt tcctaaaggc gatatcacac tgggagatgt tattagcaca ttccccttta
     1261 ataatgacct catcgagatg gatttgacgg gccgcgatct caaatcattg atgacgcatg
     1321 caaccaatct aactaacggt gtgttacaag tttctaaaag tgttgcggtt gtctatgaca
     1381 gcaaaaaacc cctcaaccaa cggttaatct ctttcaccat taacggcaaa cccgtggaag
     1441 ataatcaaac atatcgtatt gccactcact ccttttgtgc cagtggtggt gacggttttg
     1501 aagcattttt gaatggaaaa aatgtgaaga cgataccggg aacaacctcg gcggaatcta
     1561 tcatcgatta tttcaaaaat cataaacctg tcaccccaga cttaactaaa cgagtcatgg
     1621 acgtcgccaa ataaaatctc atttatcagt caatttattt aacaaggaat gcgaatgaaa
     1681 acagcgcagc gtactttatt tgccgcaaca ttgctgacta ttttttcgac ctcagttatg
     1741 gcgcaggatg tcacgattat ttataccaat gatcttcacg ctcatgtgga ttcctataaa
     1801 gtaccgtatg tcgcagacgg aaaacgcgac attggtggct ttgccaatat atccacgtta
     1861 gtaaaacaag agaaagccaa aaataaagcc accttttatt ttgatgctgg agactatttc
     1921 accgggccat atatcagtag cctgacaaag ggcgaagcaa taattgatat tatgaatacc
     1981 atgccttttg acgcagtatc aatcggtaat catgagtttg accatggctg ggataatgcc
     2041 ctccgacaat taagcaaagc gaatttccct gttttattag ccaatgttta tcataaagag
     2101 agtgagaaac ccttctggaa caagccctac accatcctgg aaaaagatgg catcaagatt
     2161 ggtattgttg ggctacacgg cgtatttgca tttaatgaca cggtctcgga gctctctctc
     2221 cagggactcg ataatgacaa taataaccga tttgataaat cttccgcgac cctcaagaat
     2281 caggggattg aagcacgtga tgaagtgaag tatttgcagc attatatcga tgagttacgt
     2341 gataaggttg acctcaccgt ggcgctggtt catgaaggcg taccggcgcg tcagtccagc
     2401 attggcaata cagatgttag acgcgctctt gataaagata ttcaaaccgc aagcaaggtg
     2461 aaaggattgg atattctgat taccgggcat gcccacgtcg gaacgccaga accaatcaaa
     2521 gtcggcaaca ccttaatcct ttcgactgac agtggcggta tcgatattgg taaattaatc
     2581 cttgatgtca accctacagc tcgtactcat aaagtgaaga gctttgagct gaaaacggtt
     2641 tatgccgatg aatggatacc tgatccaacc acacaaaaag ttatcaatgg ctggaataaa
     2701 aaactggcgg atcttgtacg ccaaccagtc ggtgaatcat cgattgcatt aactcgtgcc
     2761 tacggtgagt catcgcaact tggcaacctg tttaccgatg caatgcttgt cgctgcaccg
     2821 actgcacaga ttgcattgat aaactccggc agcttacgtg cggatataaa tgcaggcacg
     2881 attacctttg gcgacattac cagtacattc cccttcaaga atgaacttac tgaaatggat
     2941 ctcagcggta aggatctgcg taacctactg gaacacgggg catcgcttac caatggcatc
     3001 ctgcaaatgt caaaaggcgc agaaatgcgc tataccccga agaaaccagt aggtcagcgc
     3061 atagtatctt ttaaaattaa tggtgaagag atcgttgata cgaatattta ccatgttgca
     3121 acaacgactt tccttgcact ggggggcgat ggttttctgg cattcaaaga agggaaaaat
     3181 gttcaggtcc gcgccggaaa taacatgtcc gatgtcgtga tcgattattt gaagaaaggc
     3241 cacaagatta cgcctgcaca g
//
LOCUS       DAZGAP010000328         3224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGAP010000328 DAZGAP010000000
VERSION     DAZGAP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.330"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(62..372)
                     /locus_tag="ACHLAM_005134"
                     /pseudo
     CDS             complement(62..372)
                     /locus_tag="ACHLAM_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(756..1565)
                     /gene="iolB"
                     /locus_tag="ACHLAM_005135"
     CDS             complement(756..1565)
                     /gene="iolB"
                     /locus_tag="ACHLAM_005135"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFO7856504.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            complement(1590..3095)
                     /gene="iolA"
                     /locus_tag="ACHLAM_005136"
     CDS             complement(1590..3095)
                     /gene="iolA"
                     /locus_tag="ACHLAM_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFO7856505.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
BASE COUNT          850 a          798 c          741 g          835 t
ORIGIN      
        1 aaatttcatt aatttctatt cataaacagg ttactctgat atgggagcaa caaccattcg
       61 gctatattat ctttttcatg ttggtgcttt tattctgtga ggtgtgctgg catggtggac
      121 acaaccattt gttcttttta gctgccattt gataatattt tcctgaagct ttccggggat
      181 atattcccca gacgggagtg gcggcgctga cggttgtaga atatctcaat gtatacccag
      241 tattactgag atgacttcat tccggctact aaaatggtag tggttcagac tttcattctt
      301 cagagttccc caaaagcttt ccataggggt tttgtcgtaa cagttccctt tacgagacat
      361 tgatgttttc agagctaact gcgatagact ccttcaactt caacttcaac ttcaacttca
      421 acttcaactt caacttcaac tgagaaacgg atgatttcac gatagactgc ccaacgtatt
      481 gagcccgtta ggaagccaga gtaacaagcc gccgtgtacg gcatttctct cctaattcgg
      541 cagaaccaaa aatattgtca gctcaggagt aagaatcaga aaaaaccata acagaacacc
      601 agaaacagga tattatcaga cagagaaaaa attcaaaaaa atccaccgat tctgttgcct
      661 gtggatttgt gtataagagg cagaaccctg ttggggactg tgcacaaaaa agccagctgc
      721 agaggacaac tggcaaagga atatttaacg tattgttaac gcggatactc tgacgaatta
      781 atccatgcgt gatcagcttc ccatgtgaat tgccatttgc gtaatggccc ggccatcaca
      841 ttcagataat agttatcgta acctgcgata gtggccaccg gatgatagcc tttcgggact
      901 tttaccacat cgcggttata tactgccata cattcgtcca gactgcgatc ggcggtgtag
      961 actcgctgta agctgaatcc ctggggtgga tcgaaacggt ggtagtaggt ttcttccaga
     1021 taggtttcct gcccttcaac tgctgtgtca tgcttatggg caggccagga acttgtatcg
     1081 ccttcccttg tatatacttc cactaccagc agactgtcag caggttgagt gtctgggagg
     1141 atattatgaa ccaagcgctg attgcgccct tttcccctat gttccacccc aatatcttgt
     1201 ggtgagatca gtcttaccgg aagttcaccg aatccaggag cactgcagac agccagttcc
     1261 agatcagttt cggcagtgac ttttgcttct gtatggtgcg ggaggtaaac agaataagcc
     1321 ggaattcgct caaaaggact catgcgctgg ccaatccgat aaaaaaagtg gtcagcggcc
     1381 tttacgctgg caagtccggc aaccagaacc aggcaacgtt cgcgatcatc ggatggcaag
     1441 gttaaagatt cgccggcttt caacagccag acatcaaaac cgacatactc ccagcctgcg
     1501 ttttgcggag aaatatgttg aatatttcca gccctggtgg tgcaggtact cagcaagttg
     1561 gtcataatct tctccttcct gaacgtttat taacctagcg taggcatact gaactcggat
     1621 gcgatctgct gaccgttcgg ccagcgtgcg gtaacggttt tcatccgggt ataaaaacgc
     1681 accccatccg gtccgtggac attcagcgcc ccaaacaccg agcgtttcca gccgccgaaa
     1741 gagtgaaacg ccattggaac cggtaccgga acattgatac cgaccatccc ggcttcaacc
     1801 gattgagcaa attcgcgggc ataatggccg ttggaggtga agatcacgct accattgcca
     1861 aattcatgag agtttaccgt acgaatggcg gtggcataat ctgttacgcg tacgatgccg
     1921 agcacgggac caaaaatttc ctcacgccag atagtcattt ccggcgtgac gttatcaaac
     1981 aaggtaccgc cgacataaaa tccttcagga tagcccttca cgcaataatt tcgcccatcc
     2041 atcaccagtg ttgctccttc gctaacgcct ttgtctatat atgcaaggac tttttgttga
     2101 tgcatacgtg aaacaactgg ccccatttca ttttcgtctt tttctcgtaa ctcaccgggg
     2161 ccaatacgca gcgctgcaat aagtggtttt aggcgttcaa taagacggtt ggctgtttca
     2221 tcaccgacag cgaccaccac aggtaatgcc atacaacgtt cgcctgcgga accaaaagcc
     2281 ccacccataa tagcgttaac ggtagcatca aggtcagcat ccggcatgac aatagcgtgg
     2341 ttttttgcgg caccgaaagc ttggactcgc ttaccgtgtg cactggcggt ggtgtagata
     2401 tgctcagcga cggaagaaga tccgacaaaa ctgacggcct gaatgcgtgg atcactggtc
     2461 aacaggctgg ctgcgtcatt gccacagtga actacattga atacaccatc cggtagtccg
     2521 gcctcctgta gtagttgcgc catacgtaac gatgcggagg gcaccagcgc tggaggcttc
     2581 aggacgaagg tattgccgca ggcgagagcg atagggaaca tccacattgg caccattgca
     2641 gggaagttaa agggtgtgat gccagctaca acaccaagtg gttgcattaa tgaaaagctg
     2701 tcaacgcctg tgccaacatt aaaagagaac tctcctttaa tgagatgcgg aatgccgcag
     2761 gcgaattcaa caacttcgat tccgcgagtc agttcaccaa gcgcgtcgga ccatactttg
     2821 ccatgttcac tgacgatcag agccgccagt tcatctcggt gtttttccag caacacttta
     2881 aattcaaaaa ggattcttgc acggcgcaac ggtgccattt tactccatgc aggaaaagct
     2941 tcatgagcac tctgaatcgc ctccagcata tcttgccggg tgctctgggt aaccaggcga
     3001 atcacttctc cggttgcagg gttagtcacc ggtagtgttt caccactgtt gctcagtctc
     3061 gttttaccct gaataaaatt tccaactgtt tccatcactg tttaccttct tgaatcaatg
     3121 ttaacgacgt tccgactgag tgttcacagg caaagcctga tcatctacag tctaaaatca
     3181 cacagtgttt aatgtatagc ttcggattat aaatttcaga ttta
//
LOCUS       DAZGAP010000329         3222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGAP010000329 DAZGAP010000000
VERSION     DAZGAP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.331"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..962
                     /gene="cma"
                     /locus_tag="ACHLAM_005137"
     CDS             147..962
                     /gene="cma"
                     /locus_tag="ACHLAM_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HFO7856506.1"
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFAKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(1012..1365)
                     /gene="cmi"
                     /locus_tag="ACHLAM_005138"
     CDS             complement(1012..1365)
                     /gene="cmi"
                     /locus_tag="ACHLAM_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO7856507.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWPEDKGLACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEEYVEYAND"
     gene            complement(1535..2317)
                     /locus_tag="ACHLAM_005139"
     CDS             complement(1535..2317)
                     /locus_tag="ACHLAM_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7856508.1"
                     /translation="MQHLPAPIHHARDVAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVTMLKNRQA"
     gene            complement(2319..2732)
                     /locus_tag="ACHLAM_005140"
     CDS             complement(2319..2732)
                     /locus_tag="ACHLAM_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856509.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            complement(3000..>3222)
                     /locus_tag="ACHLAM_005141"
     CDS             complement(3000..>3222)
                     /locus_tag="ACHLAM_005141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856510.1"
                     /translation="ISDIISLLMIKIMSAMNLCVLLNYHITCPFPAAQIRFPAVQDLP
                     LPPHNATSRHHNMWCRIQLLMTLYVVSSP"
BASE COUNT          828 a          814 c          731 g          849 t
ORIGIN      
        1 tggtagttca ggagctgccc taccattttt aacggcatat caggcaaaca atgttatggg
       61 gagcctctct catggggttt gttcgccggc ggaggcgtta gccagcggcg aactctgtta
      121 tcttgttaac ttataaggag ttatgtatgg aaaccttaac tgttcatgca ccatcaccat
      181 caactaactt accaagttat ggcaatggtg cattttctct ttcagcacca catgtgcctg
      241 gtgctggccc tcttttagtc caggttgttt atagtttttt ccagagtcca aacatgtgtc
      301 ttcaggcttt aactcaactt gaggattaca tcaaaaaaca tggggccagc aaccctctca
      361 cattgcagat tatatcgaca aatattggtt acttctgtaa cgccgaccga aatctggttc
      421 ttcaccctgg aataagcgtt tatgacgctt accacttcgc aaaaccagcg ccaagtcaat
      481 atgactatcg ctcaatgaat atgaaacaaa tgagcggtaa tgtcactaca ccaattgtgg
      541 cgcttgctca ctatttatgg ggtaatggcg ctgaaaggag cgttaatatc gccaacattg
      601 gtcttaaaat ttcccctatg aaaattaatc agataaaaga cattataaaa tctggtgtag
      661 taggtacatt ccctgtttct acaaagttca cacatgccac tggtgattat aatgttatta
      721 ccggtgcata tcttggtaat atcacactga aaacagaagg tactttaact atctctgcca
      781 atggctcctg gacttacaat ggcgttgttc gttcatatga tgataaatac gattttaacg
      841 ccagcactca ccgtggcatt atcggagagt cgctcacaag gctcggggcg atgttttctg
      901 gtaaagagta ccagatactg cttcctggtg aaattcacat taaagaaagt ggtaagcgat
      961 aatataaggg ccggggttcc ggccttttta tgcagccttt atagcagtgt attagtcatt
     1021 cgcatattca acatattcct cctcacaatg gtacatgcca taatacattc tcttaccctc
     1081 cgggccttca gctttaaatg ggacaagaag tgttttttca tcttcaacat ctgttaaact
     1141 tctctcaatc ttttcccatg taatttttgg gatttctgta ccaagaagtt gtacatcgtt
     1201 gtcccagcgt ttcattcttt gcaggtaatc attttttaca aacgttctgg cctgttcatc
     1261 gctgacctga tagcatgcca gacctttatc ttcaggccag aaaaaaacag cagcaagagc
     1321 aataatcgtt aaaataaaaa taaatttcat gctaattact ttcataaaat ctccacaact
     1381 tctgtcgttc attcgatata aaaaaacatt acgaatatat ccgtacaata tgagcatact
     1441 gtgaagtggc tttaacaatt atgaaataat acaagagatc acatattgca gtggtctctt
     1501 ttttatagaa gaaatcacaa ttcataatta tgatttatgc ctgcctgttt ttcagcattg
     1561 tgacagcatc ggactcaggc atcaaaaact gcacccggtg ccgggcagcc acatccagtg
     1621 caaacacctt cgtgtagacc tccgttgagc tgatggactt atgccccatc aggctctgca
     1681 ggaccttcag cggtataccg gcatacagca tgtgcatcgc ataggaatgg cggaacgtat
     1741 gtggcgtgac cgggacagag aacgtcacac catcagcggc agcggcggca accgcttccc
     1801 caatccaggt cctgaccgtt ctgtccgtca cttcccagat ccgcgctttc tctgtcctgc
     1861 ctgttcgttt attacgccgt tccatgggga ttttcagtgt ggccaccatc gtctgcagct
     1921 ggctgacgta ccaggagtcg gagagcggaa ccagccggtg agtctgctga ccggctggca
     1981 ttcttcctgc cgtcctggcg gctttttctg tccgctgctt cagagtagcc agctgcacaa
     2041 acggatacgg gggcgtgagc gaaaaatccc cccgcgtcag cgccagtgct tcattaatgc
     2101 gcgcaccggt gttccacagt gtggccagca gcatcttgcg gcgcagatcc gggacgtaat
     2161 ggagcagggc gctcacttca ggggccagca ggtattttgg cagttcatca tgaaccatcg
     2221 acatctggcg gagtgccagc gctgccggat aatcgatggc aacaggaagc tgagcgacat
     2281 cccgggcatg gtggatcggt gccggcaggt gctgcatatc agtgttcact ccacggggaa
     2341 acgtcgggca ggttttccac aacacggtac tttcccggat aacggagctg gttaccgcca
     2401 cgcgtccaga cggtgacaac aaaaccccct ccctggttgt tttcggggag caggcgatcg
     2461 cactgctcac tcagcatgtg gatcacaaac ggcgcatcgc tgccgtcttc aaataccagt
     2521 tccagcgcat cgcggccatg cagcgttccc ttactgatga tgacgtactc agccccccgc
     2581 atctccctga gtaaatgttt tgccgcatcc gggacaagca gcctggctgc gccggcattc
     2641 cagctgaggt agacatatcc ggcctgcgcc tgtacagact gccagtaatc cgttccggcg
     2701 acagcctgac cttcattctc aataaaaaac atgggtgact cctttttctt ttccgggcag
     2761 ggacggtcat gcgataacgt catccatcat cactgccctt accgtcacgc cgtctgataa
     2821 catcatgccg ttatcatcat caccatgata acaagcatcc atataaccac gccgttaaat
     2881 taccggagag ccgtttccgt cagatccgac tttccggaat atcacaactt tccggaagtg
     2941 taccacgctg aggaaatcat catatcgctg ttttccgccc ctgcgcgacg catcacaggt
     3001 cagggagatg acacaacata tagtgtcatc agcagctgaa tccggcacca catgttgtga
     3061 tgacgggagg tggcgttgtg tggggggaga ggaagatcct gaacagccgg aaaccggatc
     3121 tgcgcggcgg gaaacgggca ggtgatatga taattaagca acacacataa attcatagca
     3181 gacatgattt taatcatcag taatgaaata atatctgata tt
//
LOCUS       DAZGAP010000330         3205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGAP010000330 DAZGAP010000000
VERSION     DAZGAP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.332"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..467
                     /gene="ehxC"
                     /locus_tag="ACHLAM_005142"
                     /gene_synonym="hlyC"
     CDS             <1..467
                     /gene="ehxC"
                     /locus_tag="ACHLAM_005142"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO7856511.1"
                     /translation="CSPLHKKWPLSVFAINVIPAIQTNQFALLIKDELPVAFCSWASL
                     DLECEVKYINDVTSLYAKDWLSGERKWFIDWIAPFGHNMELYKHMRKKYPYELFRAIR
                     LDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNRQDFNFNIEKEN"
     gene            469..>3205
                     /gene="ehxA"
                     /locus_tag="ACHLAM_005143"
     CDS             469..>3205
                     /gene="ehxA"
                     /locus_tag="ACHLAM_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO7856512.1"
                     /translation="MTVNKIKNIFNNATSTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADDLGIEIHRTERDDTAIANQFFGTAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PNLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSNLIQFVEKDNKHYQARIISELGDKDDIVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDRDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVE"
BASE COUNT         1046 a          498 c          761 g          900 t
ORIGIN      
        1 cctgttctcc tctgcataaa aaatggccat tgtctgtttt tgctattaat gtcatacctg
       61 ctattcagac aaatcaattt gctctcttaa taaaggatga gcttcctgta gctttttgta
      121 gctgggccag cttagatctg gagtgtgagg taaaatatat aaatgatgta acatctttgt
      181 atgcaaaaga ctggttgtcg ggagaaagaa agtggtttat tgactggata gctccatttg
      241 gacacaacat ggagttatat aaacacatga ggaaaaagta tccttatgag ttattcagag
      301 caataaggtt agatgagtac agtaaaacag ggaaaatagc tgaatttcat ggagggggga
      361 tagataaaaa actggcatca aaaatattca ggcaatacca tcatgaactt atgtctgaag
      421 taaaaaacag acaagatttt aattttaata ttgagaaaga aaactaatat gacagtaaat
      481 aaaataaaga acattttcaa caatgcgaca tcgactacaa aatcagcatt caatacagca
      541 tcatcaagcg tacgttccgc tggaaaaaaa ctcatattat taatacctga taattatgaa
      601 gctcagggcg tgggtattaa tgagttggtc aaagctgctg atgatcttgg aatagaaata
      661 caccgtactg aacgagatga tacagcgatt gcaaaccagt tttttggtac agcagaaaaa
      721 gttgtaggat taactgaacg tggtgttgca atattcgcac cacaacttga caaacttctg
      781 cagaagtatc agaaagttgg gagtaaaata ggaggaaccg ctgaaaatgt aggtaataat
      841 ctgggaaaag ccggaacagt tctctcagca ctacagaatt ttacggggat tgctttatct
      901 ggcatggctc ttgatgaatt gctgagaaaa caacgggaag gagaggatat aagtcagaat
      961 gatattgcca aaagtagtat tgaacttatt aatcagcttg tagatacagt atcaagtata
     1021 aacagtaccg ttgattcatt ttctgagcag cttaaccagc ttggctcatt tttatccagt
     1081 aaacctcgct taagttctgt tggtggaaaa ttacaaaatt taccgaacct gggccccctg
     1141 ggggatgggc tggatgttgt ctccggaatt ctttctgctg tatcagcaag ctttattctg
     1201 ggaaacagtg acgcacatac aggaacaaaa gctgcagcgg gtatcgaact gacaactcag
     1261 gttcttggaa atgttggtaa agctgtttcg caatatattc tggctcagag aatggcacag
     1321 ggattatcga caacagctgc aagtgcgggt ctgatcacat cggctgttat gctggctatc
     1381 agtcctcttt ctttcctggc tattgcagat aaatttgagc gagctaagca gcttgaatca
     1441 tattctgaac gatttaaaaa attgaattat gaaggggatg ctttactcgc agcctttcat
     1501 aaagaaaccg gagctataga tgcagccctg acaacaataa atactgtcct gagttctgta
     1561 tctgcgggag ttagtgccgc ctccagtgca tccctcatag gggccccgat aagcatgctg
     1621 gtgagtgcat taaccggtac gatatctggc attctggaag catcaaaaca ggctatgttt
     1681 gagcacgttg cagataaatt cgctgcccgg atcaatgaat gggaaaagga gcatggcaaa
     1741 aattattttg agaatggcta tgacgcaaga catgctgcgt ttttagaaga ttctctgtct
     1801 ttgcttgctg atttttctcg tcagcatgca gtagaaagag ctgtcgcaat cacccagcaa
     1861 cattgggatg agaagatcgg tgaacttgca ggtataaccc gtaatgctga tcgcagtcag
     1921 agtggtaagg catatattaa ttatctggag aatggagggc ttttagaggc tcaaccgaag
     1981 gagtttacac aacaagtttt tgatcctcaa aaagggacta tagacctttc aacaggtaat
     2041 gtatcaagtg ttttgacatt tgtaacacca acatttaccc caggagaaga agtcagagaa
     2101 agaaaacaga gtggtaaata tgaatatatg acatctctta ttgtaaatgg taaggataca
     2161 tggtctgtaa aaggcataaa aaatcataaa ggtgtatatg attattcaaa tttgattcag
     2221 tttgttgaaa aggataacaa acactatcag gcgagaataa tttctgagct cggagataaa
     2281 gacgatatag tttattctgg ggcaggctca tcagaagtat ttgctggtga aggtcatgat
     2341 accgtatctt ataataagac ggatgttggt aaactaacaa ttgatgcaac aggagcatca
     2401 aaacctggtg aatatatagt ttcaaaaaat atgtatggtg acgtgaaggt attgcaggaa
     2461 gtcgttaagg aacaggaggt gtcagtaggg aagcgaacag agaaaataca atatcgtgat
     2521 tttgaattca gaaccggtgg aattccttat gatgtgatag ataatcttca ttctgttgaa
     2581 gaactcattg gcggaaaaca tgatgatgaa ttcaaaggcg gtaagtttaa tgatatattc
     2641 catggcgcag atgggaacga ttatatcgaa ggtaattatg gtaatgatcg actatacggc
     2701 gatgataggg atgattatat atccggagga cagggagacg accagttatt tggtggtagt
     2761 ggaaacgata aattgagtgg aggggatggt aataattatc tgacaggagg aagcggtaat
     2821 gatgagcttc aggcacacgg agcttataat attctgtcag gtggtactgg tgatgataaa
     2881 ctttatggtg gtggtggtat tgaccttctg gatggagggg aaggtaatga ctatctgaat
     2941 ggtggttttg gtaatgatat ttatgtttat aggcaaaact atggtcatca tacaattgca
     3001 gatgaaggag gtaaaggaga tcgtctgcac ttatctgata ttagctttga tgatatcgca
     3061 tttaagagag ttggaaatga tcttatcatg aataaagcca ttaatggtgt actttcattt
     3121 aatgagtcaa atgatgtcaa tgggataaca tttaaaaact ggtttgcgaa agatgcctca
     3181 ggagcagata atcatcttgt tgagg
//
LOCUS       DAZGAP010000331         3200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGAP010000331 DAZGAP010000000
VERSION     DAZGAP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.333"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2217
                     /locus_tag="ACHLAM_005144"
     CDS             <1..2217
                     /locus_tag="ACHLAM_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFO7856513.1"
                     /translation="APVKAPPKEWLANNKKNQSVMQNEDSEDDNLATFTLSGGRNVEY
                     RITATQRQNFSKTTGALARWIFPGEMITVSGIEVTGGHFYLGQRMKPAGHNSGGYYDG
                     NNEASLVDDTLKIYPTPYIYEDSSLGYWPSFSSLSPEARGAYLSWLASDRCDTSCPIG
                     YVFIYLYGLERRALVDSRDNNISDDEFYSLFKEICRLRSVFNENRSFRNYSSQLLEAM
                     SILRPNENLAAALNDDSEFSNSMQFRLILAKTVDTGAPVPADLALTWVTNHAEYSLRT
                     PARRCAKEFAALFKRRYTLKYGEGMVVKPNKARLRLDYTPASPSLRGIRLPVPDLPDP
                     GALKGPVQKLMAIADICTGELDAYSRYLGRKGTSANDTAAILLLPSEIVNESAEKILS
                     TFKHWADDAIRSKGGIVSVADYWSHMNATCPAKINKKEADLMQAFAQKMGYCLAPDPY
                     HHHVKADVDGVLVLFPAGERGRFSPYPEFITAVLTLRLGSVVALIDNSLDQAEQKVLE
                     NAINNNASFSDDEKRSLHAYLTWQLHTPANMTGMKSRIELLGAAEKSAVGKVIVSVAC
                     SDGRITPAEIKQLEKIYTSLGLDPSSVSSNIHQHSATETALVSSVPANQSTAGFTLDA
                     NVLARHESATDDVRKLLNSIFTEEEPEQPESAPASLTGEGGLDSAHSQLYRRLLEKEQ
                     WSRKEATELCSNWNLMLGGALEVINDWSYAVVDAPVLEDADDHIWIDLEIAKELEG"
     gene            2221..2622
                     /locus_tag="ACHLAM_005145"
                     /pseudo
     CDS             2221..2622
                     /locus_tag="ACHLAM_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            complement(2593..2801)
                     /locus_tag="ACHLAM_005146"
                     /pseudo
     CDS             complement(2593..2801)
                     /locus_tag="ACHLAM_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2836..2970)
                     /locus_tag="ACHLAM_005147"
     CDS             complement(2836..2970)
                     /locus_tag="ACHLAM_005147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856514.1"
                     /translation="MSEIKIIGIELAKTNFYPFNINDYGKTVGKIKFSRSNLLNLLVQ
                     "
BASE COUNT          896 a          712 c          739 g          853 t
ORIGIN      
        1 gctcctgtga aagcaccacc taaagaatgg cttgctaaca acaaaaaaaa tcagtctgta
       61 atgcaaaacg aggacagcga agatgacaat ctggctacgt ttacactaag tggcgggcgg
      121 aatgtcgaat acaggataac agccactcaa cgccagaatt tctcaaaaac tactggtgct
      181 ttggctcgtt ggatttttcc gggagagatg atcacagtca gtgggataga ggttaccggc
      241 ggtcacttct atcttgggca gcgtatgaaa cctgccgggc ataactcagg cggttattat
      301 gatggcaata acgaggcatc gctagttgac gacacgctta aaatataccc cacaccttac
      361 atttacgaag atagttcatt agggtattgg cccagttttt cctcactttc acctgaagct
      421 cgtggtgcgt acctgagttg gcttgccagc gacagatgcg acacttcctg cccaattggt
      481 tacgttttca tttatctgta tggtctggaa agaagggcgc ttgttgacag cagggacaac
      541 aatatatccg acgatgagtt ctattctctt ttcaaagaga tctgccgtct cagatcggtt
      601 ttcaatgaaa accgatcttt ccggaattac tcatcccagt tacttgaagc gatgtccatt
      661 cttcgtccga acgaaaatct tgcagcagct ctcaacgatg attcggagtt cagcaatagc
      721 atgcagttca ggttaatcct ggcaaagact gttgatacag gagccccggt tccagcggat
      781 ctggctctga cgtgggtgac aaaccatgct gaatactcat tacgaacccc cgcccgccgc
      841 tgtgcgaaag aatttgctgc actatttaaa cggcgttaca cccttaaata cggtgaaggg
      901 atggtagtga agccgaataa agcgcgtctg cgtttagatt acacgcctgc aagtcccagt
      961 ttgcgaggta ttcggcttcc tgttcccgac ttgcctgatc caggtgcatt gaaaggccca
     1021 gttcagaagc ttatggccat tgccgatata tgcacaggtg aactggatgc ttacagtcgt
     1081 taccttggta gaaaaggtac ttcagcaaat gataccgctg caattttgtt gctgccttca
     1141 gaaatcgtta acgaaagcgc agaaaaaata ttaagtacat tcaaacactg ggctgatgac
     1201 gcaatccgca gtaagggtgg gatagtttca gttgctgact actggtcaca tatgaatgcc
     1261 acctgtccgg cgaagatcaa taaaaaagag gccgacttaa tgcaagcctt tgcacagaaa
     1321 atgggatact gtctggctcc tgacccctat caccaccacg taaaggcaga tgtcgatggt
     1381 gtacttgtct tattccctgc tggtgaaaga ggacgtttct caccttatcc tgaatttatc
     1441 acagctgtat tgacgctccg attaggttcg gtagtcgcct tgattgacaa ttctctcgat
     1501 caagccgaac agaaagtgct tgagaatgcc atcaacaaca atgccagctt cagcgatgat
     1561 gaaaagcgct ctctgcacgc atatttaacg tggcagcttc atactccggc gaatatgacg
     1621 ggaatgaaaa gccggattga attactcggt gcagcggaaa aatcagctgt gggtaaggtt
     1681 atcgttagtg tcgcttgctc tgacggcaga attacgcctg ccgaaatcaa acagcttgag
     1741 aagatttata caagcctggg actggatccc tcatccgtat caagcaatat ccatcagcat
     1801 tccgcaactg aaactgctct cgtttcatct gtaccagcta atcagtcgac ggcagggttt
     1861 acgctggatg ccaacgtact tgcccgccac gaatctgcca ctgatgatgt tcgtaaatta
     1921 ctgaactcaa ttttcactga agaagaacca gaacaaccag aaagcgctcc agcctcatta
     1981 accggggaag ggggacttga ctccgctcat agccaacttt atcgcaggtt gctggagaaa
     2041 gaacaatggt ccagaaaaga ggcgacagag ttgtgtagta actggaacct gatgctcggc
     2101 ggtgcgcttg aggtgattaa cgattggtcc tatgcggtgg ttgatgcacc agtgctggaa
     2161 gatgctgatg atcacatttg gattgacctg gaaatagcca aagaattaga gggttagtga
     2221 atgtctgtaa cacgtataag agtgaaagag cgcgacgcta tcattcagtc acttaagtcg
     2281 ggagtaacgc caagaatagg tattcagcat atccaggttg gccgcgtgaa tgagataacc
     2341 gccctccatc aggatattga acggattgct gatggcgggg caagcttcag acttatcatt
     2401 ggtgagtacg gttccgggaa aacattcttt ttaagtgttg tacggtctat tgcgttagag
     2461 aagaagctgg tcacggtcag tgcagacctt tctcccgaca gacgtatcca ctcatcggga
     2521 ggtcaggctc gtaatctcta ctcagagctt atgaaaaata tgtccaccag aaacaaacca
     2581 gatggcaatg cattacttag cgttgtggaa aggtttgtta cagtcaaggc agtcaccaaa
     2641 ctcgataata aagcggctca tttccatacg tctgtcctta aggggcattg ttcatttctg
     2701 tgaccaagcc gcaccacctt ttttactgat tcgtctgtcg ggaacacttt gcgtttcttg
     2761 atgacataac ggatcacact attcaacgac tctatggcat tttgcacata cttccatagc
     2821 aactgtcatc ggtagctact gaaccagcaa gttaagtaag ttactacgtg aaaattttat
     2881 ttttccaacg gtttttccat aatcattaat attaaaagga taaaaatttg ttttagccag
     2941 ctcgatacca atgattttta tttcagacat gatatccttc ttacatttca ttgattaaag
     3001 tttatccatc atggtatgat ctgcttctac cgtgaaggag gggctgacac tcaatggacc
     3061 aaaaactcac gttaaacaat tgtagcaaag gaaagttaaa ggatcctgcg aggatccttt
     3121 gataggtgtt aaaattgcat tcttattacc atataagatc agttatctct cagtagctgt
     3181 gagcatatta aatttatatt
//
LOCUS       DAZGAP010000332         3133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGAP010000332 DAZGAP010000000
VERSION     DAZGAP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.334"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..1745
                     /gene="ydeN"
                     /locus_tag="ACHLAM_005148"
     CDS             63..1745
                     /gene="ydeN"
                     /locus_tag="ACHLAM_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7856515.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEKLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1797..2954
                     /gene="ydeM"
                     /locus_tag="ACHLAM_005149"
     CDS             1797..2954
                     /gene="ydeM"
                     /locus_tag="ACHLAM_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7856516.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHTENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETISYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2920..3042)
                     /locus_tag="ACHLAM_005150"
     CDS             complement(2920..3042)
                     /locus_tag="ACHLAM_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856517.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          953 a          685 c          636 g          859 t
ORIGIN      
        1 atccacttat ttctcttcgt aaaattactt tggaattaag tacaataaga agaggaacat
       61 ttatgaagtc tgcattaaag aaaagtgtcg taagtacctc gatatctttg atactggcat
      121 ctggtatggc tgcatttgct gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa
      181 caaacgttgc tttctcagac tttacgccga ctgaatacag taccaaggga aagccaaata
      241 ttatcgtact gactatggat gatcttggtt atggacaact tccttttgat aagggatctt
      301 ttgacccaaa aacaatggaa aatcgtgaag ttgtcgatac ctacaaaata gggatagata
      361 aagccattga agctgcgcaa aaatcaacgc cgacgctcct gtcattaatg gatgaaggcg
      421 tacgttttac taacggctat gtggcgcacg gtgtttccgg cccctcccgc gccgcaataa
      481 tgaccggtcg agcgcccgcc cgctttggtg tctattccaa taccgatgct caggatggta
      541 ttccgctaac agaaactttc ctgcctgaat tattccagaa tcatggttat tacactgcag
      601 cagtaggtaa atggcacttg tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa
      661 cgcgtgacta tcatgacaac ttcaccacct attctgcgga agaatggcaa ccgcaaaacc
      721 gtggctttga ttactttatg ggattccacg ctgcaggaac ggcatattac aactcccctt
      781 cactgttcaa aaaccgtgaa cgcgtccccg caaaaggtta tatcagcgat cagttaaccg
      841 atgaggcaat tggcgttgtt gatcgtgcca aaacacttga ccagcctttt atgctttacc
      901 tggcttataa tgctccgcat ctgccaaatg ataatcctgc accggagcaa tatcagaagc
      961 aatttaatac cggtagtcaa acagcagata actactacgc ttccgtttat tctgttgatc
     1021 agggtgtaaa acgcattctc gaaaaactga agaaaaacgg acagtatgac aatactatta
     1081 ttctctttac ctccgataat ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc
     1141 aaaaaggcta taagagtcag acctatcctg gcggtactca caccccaatg tttatgtggt
     1201 ggaaaggaaa acttcaaccc ggtaattatg acaagctgat ttccgcaatg gatttctacc
     1261 cgacagctct tgatgcagcc gatatcagca ttccaaaaga ccttaagctg gatggcgttt
     1321 ccttgctgcc ctggttgcaa gataagaaac aaggcgagcc acataaaaat ctgacctgga
     1381 taacctctta ttctcactgg tttgatgagg aaaatattcc attctgggat aattaccaca
     1441 aatttgtccg ccatcagtca gacgattacc cgcataaccc caacaccgag gacttaagcc
     1501 aattctctta tacggtgaga aataacgatt attcgcttgt ctatacagta gaaaataatc
     1561 agttaggtct ctacaaactg acggatctac agcaaaaaga taaccttgcc gccgccaatc
     1621 cgcaggtcgt taaagagatg caaggcgtgg taagagagtt tatcgacaac agccagccac
     1681 cgcttagcga ggtaaatcag gagaagttta acaatatcaa gaaagcacta agcgaagcga
     1741 aataactaaa ccttcatgcg gcggattttt ccgccgcctt attgagcgag atagcgatgc
     1801 acgttacagc caagccctcc agttttcaat gtaatctcaa atgtgattac tgtttttacc
     1861 ttgaaaaaga gtcgcagttt actcatgaaa aatggatgga tgacagcact ctgaaagagt
     1921 tcatcaaaca atatatcgca gcgtctggca atcaggtcta ttttacctgg caaggcggtg
     1981 aacccactct ggctggcctg gattttttcc gtaaagttat tcactatcaa caacgctatg
     2041 caggccaaaa acgtattttt aatgcattac aaacgaatgg cattttattg aataatgaat
     2101 ggtgtgcctt tctcaaagaa catgagtttc tggttggtat ctcgatcgat ggcccccaag
     2161 agctacatga ccgttacaga cgcagtaatt caggtaacgg tacttttgca aaagtgatag
     2221 cagccatcga gcgtctgaaa tcatatcaaa tagagtttaa tacgttaacc gttattaata
     2281 acattaatgt ccattaccct cttgaggttt atcatttttt aaaatctatc ggcagtaaac
     2341 atatgcaatt tatcgaattg ctcgaaaccg ggacgccgaa tattgatttc agtggtcata
     2401 ctgagaatac attccgtatc attgattttt ctgtgcctcc cacggcttat ggcaagttta
     2461 tgtcaaccat ttttatgcaa tgggttaaaa acgatgtggg tgaaattttc atccgtcagt
     2521 ttgaaagctt tgtcagccgt tttttgggga atgggcatac cagttgtatt ttccaggagt
     2581 cctgcaagga taatctggtt gttgaaagta atggagacat ttacgaatgc gaccattttg
     2641 tctatccaca gtacaaaatt ggaaacatta ataaatcgga actcaaaacg atgaacagtg
     2701 tacaactgac agcgcaaaaa aaacggattt cagcgaaatg tcagcaatgt gtatataaac
     2761 ctatctgcaa tggtggttgc cctaagcatc gtattactaa agtaaacaat gagactattt
     2821 cctatttttg cgaaggttat aaaatccttt tttcaaccat ggtaccttat atgaacgcca
     2881 tggtggagtt agccaagaac agagtaccgc tttaccacat tatggatgtt gcaaaacaaa
     2941 tggagaataa ttaataacgc tccttctttg ctccagtctt agcctccgtc ttatgacctt
     3001 aacaaagcac ccgacataaa ttcctgttgg gtgtttcgtc atcatcatct tcccttgcga
     3061 aaattctggc ttacgcggca tgcgtcaggg cattagcaat acccttgatt atcaatagca
     3121 taaccataac cac
//
LOCUS       DAZGAP010000333         3073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGAP010000333 DAZGAP010000000
VERSION     DAZGAP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.335"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(401..1879)
                     /locus_tag="ACHLAM_005151"
     CDS             complement(401..1879)
                     /locus_tag="ACHLAM_005151"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO7856518.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWTSLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYDHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTILGHITPQAALATHFPAGLSVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMIRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(1906..>3073)
                     /locus_tag="ACHLAM_005152"
     CDS             complement(1906..>3073)
                     /locus_tag="ACHLAM_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO7856519.1"
                     /translation="KFMGFSNTEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSA
                     MIITGLLGLLMATYPPLWVMLCIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGW
                     MEGLRGVGVMSLAVFTMWVFSRFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNN
                     LRSTNNEEKQSFQLSDILAVLRISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSL
                     VAASYMGIVINKIFRALCGPLGGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSN
                     PQSVAMGIGLILLLGFTCYASRGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVY
                     PIIGHWQDTLPAAEAYRNMWLMGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          819 a          779 c          682 g          793 t
ORIGIN      
        1 taacagtgta ttttttaagc ttgttattca ttgattaagt aataaatctg gaaatttgtc
       61 tttgttttga ggctaataag cggttttaca taaccgcctc tatacgctgt tgatgaattg
      121 ttcttatgaa taaagatatc cagttcatac tttaaatgaa aattgataaa gtacgattcg
      181 tattgtcttt tattctaaag acatcgagtg tagttaatat tccttgtaaa aacagggata
      241 aaccgaacta gttaaagttt ttattatgtt ccctgcatca gcggggataa accggacttg
      301 ttatcatttt ttaagttgtc gaacttatgt tccccgcgtc agcggggata accattcagg
      361 tatgttcagg ccgggcagtt tcccgcccgg ccttcctcac ttacgcattc gaccagctct
      421 ggatcgaatc cgcattcccc aattccccat gcatcacttc atacgatttt ttcaggatca
      481 catcagtatg tttggttagg tctttgaaaa tgcctttatt catcgcgtcg tagcgtttgg
      541 cattgctctc aatcggtata aagatatctt tcacgcgaat cattttgtcg acagccgttg
      601 cgtaatccgg gtatagcccc agacctaccg cggtattaat cgctgccccc agacttgcac
      661 aaccgttaat ggcgttacgt cgtgccggaa ggttgaacac gtcggcaaaa atctgcataa
      721 acagatcgct gttcgaaccg ccgccagtaa tgattacatg cttcgcaaag tgattcattt
      781 cattacacat attgtcgtaa ttgttcttca gcgtcagcgc cacgctttcc agtatcgaac
      841 gatatatcca tgcgtaatcc atactggaat caaagccgat cataatcccg cgtttgtacg
      901 gttcccacgg attggtcagc cagtccagca ccgtcatcag cccattacag ccaggtggca
      961 cactagaggc tttcttgttg agtaaatctt ccggtgaaag atcctgcgcc ctggcatcct
     1021 gaattaacga ctcgcctaac atgtcgcgca gccagctcac cgtccacatg cctttgcgaa
     1081 taccgtaacc ttcatacagc aatgtttgcg gaatagaaga cataatcggc cagtacgcca
     1141 ccggatcttt cggcaaggct ttgccgttca tcatcaatgc gatgtaagtg ccaagagaaa
     1201 ttaccgcagt ttcatcatcc agtaacccgg ctcccagagc ttctaccggt ttatcactgg
     1261 tggtacaaac aaccgacagc ccagccggga aatgtgtcgc cagcgcagct tgtggtgtga
     1321 tatgcccaag aatggtgcca ggcatttgca catcaaacag catatgacgg gggatattaa
     1381 acttatccat taccgcagca tcttcactcc atgtccagct cttataatcc accggccact
     1441 gaccaaaata gttggcgata ttatctttaa actcgccggt taagcgatgc gtcagataac
     1501 ccgaaaaaga ggtgacatag gccacatctg gattggtatg atcgtaggga cgtgttacac
     1561 gtgcatcctg ccagctaatc aatggcgcag cgggcgtgcc atcggctttc aataacgcac
     1621 gacagcaacg gatggagccc agaccaatac cgacaatatc ttctttattt ccggcaaact
     1681 ggctcatcaa atcgtgaccg gcaaagcata acgatgtcca taaatcgtcg tcaggatgtt
     1741 cggcagtatc ggcatctggc gtatgcatcg gctgcaataa gcctttacct tcgcaaacca
     1801 cattaccttc cagatcgtac atcaccactt ttgtgctctg acttccgcca tcaatcccta
     1861 tgatgtattt tttcgacatg atttttctcc tgtaattttc ctggcttact tgctgctagc
     1921 cattgcgggg gcgctatcag cagtacgaat tttttggaac agcaaaaagg taaagacaat
     1981 caccatgcca agcgccgcca tgcccatcag ccacatattg cggtaggctt ctgctgcggg
     2041 cagggtgtct tgccagtggc cgataattgg gtaaacgaag acatccggca ggaatccaat
     2101 caccgaacaa atacctaccg tggtgcccat aatgtaagac ggtgttctcg cttcgcctgg
     2161 gcaggcccag tacagcccgc gtgaggcgta acaggtgaat cccagcagta aaatcaggcc
     2221 aatccccatc gcgaccgatt gcgggttaga gttcgtgacg agcagggcag ttaacgccag
     2281 caggccgagt acggaaagga tttggatcac gcgggtaggg gatttcactt tactgtaggt
     2341 ggtgataatg ccgccaagtg ggccgcacag cgcgcggaag attttgttga tcacaatccc
     2401 catgtagctc gccgccacca gcgacatgcc gtacatttcg gtcagatagt tggtggagta
     2461 actcagaatg gcgtagatgg tgaagacacc aaaaatcacc atgctgcaat accaggtggt
     2521 actgatacgc aaaacggcca ggatatcgct aagctggaat gactgttttt cttcgttatt
     2581 ggtactgcgc aggttgttgt tatcgctaac aaaaaaccag cacagaatcc ccaacaagat
     2641 gtaaaccaca ctgtagataa taatgactgt tttcaggctg gcgctgtcat ccggcgcaaa
     2701 gcgagaaaag acccacatgg taaacaccgc cagcgacatt acaccgacgc cgcgcagccc
     2761 ttccatccag cccataattt tcccttgctc actatgatcg cccagcaatg aggcggcttt
     2821 aatcgacacc gaccacagca ttaaaatcgt cgttatcgca aaggcgacct gaatacagag
     2881 cattacccac agcggtggat atgttgccat taacagaccc agtaatccgg taatgatcat
     2941 cgcggaagta atcattttgc ggtgtgaaaa tttatcggca ataacgccgc tgggggcata
     3001 aagaataata gccgcaatac caaaggtact cattattaaa cctatctcgg tattgctgaa
     3061 tcccataaat ttc
//
LOCUS       DAZGAP010000334         3042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGAP010000334 DAZGAP010000000
VERSION     DAZGAP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.336"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..1026
                     /locus_tag="ACHLAM_005153"
     CDS             175..1026
                     /locus_tag="ACHLAM_005153"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO7856520.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTTIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1053..2042
                     /locus_tag="ACHLAM_005154"
     CDS             1053..2042
                     /locus_tag="ACHLAM_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO7856521.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQSIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2073..2966)
                     /locus_tag="ACHLAM_005155"
     CDS             complement(2073..2966)
                     /locus_tag="ACHLAM_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7856522.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
BASE COUNT          793 a          786 c          733 g          730 t
ORIGIN      
        1 attcattttt attcatcgga ctgtaaattc cggggatatc gaacttgcac gactaatctc
       61 aaccctcaaa cagatttatg aattttgttc ataacccctg acaaatcctg ccgtctaatc
      121 gagctcttcc ctttacgtta tgcttctcat gaacgataac ggaaggataa caccatgcaa
      181 aaagtaaaac tgaacaacgg tattgaaatg cccctgttgg gctttggtgt atttcagatg
      241 accgatgccg ccgaatgcga aagagcggtc attgacgcta ttaacagcgg ttatcgactg
      301 attgacaccg ccgcctctta tcaaaatgaa atccaggtcg ggaacgcact gaaacaaagc
      361 ggtatcgccc gtaacgaact ctttgtcacc acgaaactct ggttgcagga taccagttac
      421 gaaggggcca aagcccagtt cgaacgctct ctgaatcgac tgcaacttga ttacgttgac
      481 ctgtacttga ttcaccagcc atatggcgat gtccacggag cctggcgtgc catggaagag
      541 ctgcaacagg ccggtaaaat tcgcgctatt ggcgtcagta acttccatcc tgaccgactg
      601 gcagacctta tcgcttttaa taacgtcgtt ccggcggtaa accaggttga agttaaccct
      661 ttcaatcagc agctacaggc cgttccctgg atgcaaagtc gtggcattca gccagaagcc
      721 tgggcaccgt ttgcggaagg gaaaaatggt ctgttccagc atcctgttct cacgacaatt
      781 ggcgagaagt atggcaaaag cgtgggccag gttgttctgc gctggatcta ccaacgaggc
      841 attgtttcac tggcaaaatc agtgcgaaaa gaacgcatgg aagagaacat caacattctc
      901 gattttgaac tcagccctga agatatgctg caaattaccg ccctggatac cgcaaccagc
      961 gcgttcttct ctcaccgcga tcccgcaatg gtggaatggc tgactggccg caaacttgat
     1021 gtttaagccg cgataaaaga ggtattcatt caatgcaaaa acgttatctg ggtaaatccg
     1081 gactcgaagt ctccgccctt gggctcggtt gcatgggctt aagccacggc tacggcccgg
     1141 cgaccgatac ccgtcaggct atcgaactta ttcgtgctgc ggttgaacgt ggcgtcacct
     1201 tcttcgatac cgccgaagtg tatggtccgt atctgaatga agaagtggtc ggcgaagcct
     1261 taaaaccgtt tcgtgaccgc gtggtaattg ccactaaatt cggttttacc ttcggcgatg
     1321 ataataagca gcagatttta aacagccgtc cggaacatat ccgtgaagcc gttgaaggat
     1381 cattacgtcg tctgaaaaca gatgttattg acctgctgta tcaacaccgt gttgatccag
     1441 atgttcctat tgaagatgtt gcaggaacgg tgaaagacct catcgcagaa ggtaaggtca
     1501 aacatttcgg cctgtctgaa gcaggagcac aaaccattcg tcgtgcacat gcggtacaac
     1561 ctgtcaccgc cctgcaaagc gaatattcca tgtggtggcg tgagcctgaa caggaaattc
     1621 tgccgttgct ggaggaactg ggaattggtt ttgtcccctt cagcccacta ggtaaaggtt
     1681 tcctgacagg ggcgattaag ccgggaacca ctttcggcaa agatgattac cgcagcaccg
     1741 taccgcgttt cgcagcacaa tccattgaag ccaatgaaaa actggtcacc ttgttaggtg
     1801 aactggctgc ggagaaaggg gtaacgtctg cacaaatcgc actggcatgg ctattagcac
     1861 aaaagccgtg gattgttccc atccctggca ccactaaact gcaccggctg gaagaaaacc
     1921 tggccgctgc tgacattgtt ctttcgcaga aagatacgca gcagataagc gaggcgctgg
     1981 aaacaattaa aatcgtcggt gaacgttact cgcctgaaca tcaggcacgc gtaggccgct
     2041 aatacccaaa cgggtccagg ataaaggacc cgttattcgg tgtagcgaag cgcatcgatc
     2101 aacagcgcaa aagctggcgg atgctgcttg cggctggggt agtaaagata atacccagga
     2161 aaagttggac accactcctg taacacctga ataagcttgc ctgactctat atactcctga
     2221 accatgtctt cagggacaca agcgatccca aaccctgata acgcggcatc aattctttct
     2281 ggcaatggac tacacgtcag ttgcccatca accttaacgc gaagcggttt cccttctcgc
     2341 tcgaactccc agtggtaaag cccaccggca gttggcaaac gcatgttgat gcagcgatga
     2401 ttttgtagct cgtgaggcgt ttcaggcgca gggtttgcag caaaatacgc cggagctccc
     2461 accacagcca tccgcatatc tggtccaatc ctgactgcaa ccatatcttt atcaacattt
     2521 tcacccagtc gtatcccggc atcaaaacgg ccttcaacaa tatcgacaaa accgttatcc
     2581 accaccagtt cgacattgat ttcgggatac tctctgagaa aaggttttag cttcggccag
     2641 accagacttc gcgcggcatg ttccccggca gataaccgaa tattcccgga gggcgtgccg
     2701 ttcaattgaa taagtgattc cagttcctgt tcaagctcgt caatacgcgg ttcaaggcag
     2761 gcaatgattc tctcccccgc ttctgtggga gccacactgc gggtagtccg tgtcaggagg
     2821 cggatattga gcctttcctc cagagccttt attgcatggc taagtgcaga ctgagagacg
     2881 ccgagtttgc ccgctgcttt ggtaaaactt cgctctcttg ccaccacaag aaagatttgc
     2941 agttcgttga agttttcttt gagcattggc gatttccttg tagaagcatc ctcttcacac
     3001 acactcatga agggggcata gatgctatta ttttaaccac at
//
LOCUS       DAZGAP010000335         3003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGAP010000335 DAZGAP010000000
VERSION     DAZGAP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.337"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..576)
                     /locus_tag="ACHLAM_005156"
     CDS             complement(<1..576)
                     /locus_tag="ACHLAM_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFO7856523.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVFLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLPANSSSSVIISVVLMAIVGIVSIG"
     gene            769..1272
                     /locus_tag="ACHLAM_005157"
     CDS             769..1272
                     /locus_tag="ACHLAM_005157"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7856524.1"
                     /translation="MNNIAPQSPVIRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1318..1734)
                     /gene="rraB"
                     /locus_tag="ACHLAM_005158"
     CDS             complement(1318..1734)
                     /gene="rraB"
                     /locus_tag="ACHLAM_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO7856525.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1896..2909
                     /gene="argF"
                     /locus_tag="ACHLAM_005159"
     CDS             1896..2909
                     /gene="argF"
                     /locus_tag="ACHLAM_005159"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO7856526.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          881 a          691 c          659 g          772 t
ORIGIN      
        1 accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa cactacttga
       61 actattggca ggtagcatct ttgtcgagat aaagaaaaca gtccccatcc caatggccat
      121 taaaatcggc aagaaaaagg ttacccacca gaaccctttc aaagagcaat taaaactaaa
      181 tcttacgccg ttgagactgg tcattaaggc ctgataacgc agtccttttg ctgccataaa
      241 tataagaaaa accaacagta acaaagtcaa aacacagcca acaagcggca tatctgctga
      301 cactgtcatt aagattgcga aatagaaaaa aacaaaaaca agacaactaa caaaaacatt
      361 cccaccggta attccataag aaaatcgttg tccgttaact tccatattag cataaagata
      421 acgcttacat ttcattaatg cccatggtaa atagatacct agcgttataa tcgttaacaa
      481 cacattcacc acacaaataa gaaaatatct ctcacctgta ccatgaaaaa caaaagaatg
      541 ggacggaaca tccatttcat taataacttg agccataaaa attccctgaa ttaaaaaatc
      601 cattgatata atttatagat acagacagta aaatgttctg ccacgttaca atactcttat
      661 cagtaatagg ctggcaaaaa ctttattgct ttataagacg cttgcgcacg taagaagaga
      721 gtggtattaa acaaatgtat aattacttta tttttattgg gttaaactat gaataacatt
      781 gcgccgcaat cacctgtaat acgtcgcctg acgctgcagg ataatcctgc tatcgcccgc
      841 gtcattcgtc aggtatccgc cgaatacggt cttaccgctg ataaaggcta caccgtcgcc
      901 gatccgaatc ttgacgagct gtatcaagtg tatagtcagc ctggccatgc atattgggtg
      961 gttgagtacg agggtgaagt ggtcggcggc ggtgggatag cgccattaac aggtagtgaa
     1021 gcggacattt gcgaactgca gaaaatgtac tttcttcccg ccattcgcgg taaagggctg
     1081 gcaaaaaaac tggccttaat ggcgatggag caggcgcgag aaatgggttt caaacgctgc
     1141 tatctggaaa cgaccgcttt tttgaaagaa gcgattggtt tatatgaaca tttaggtttt
     1201 caacatatcg actatgcact tggctgcacg ggccatgttg attgtgaagt gcggatgttg
     1261 cgcgaactct gaaaaaatgc ccgctagtat gtgctatcgg gcattttaat caattaatta
     1321 gtggcgaacc ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccat cttcgccgtt
     1381 aggatcttca aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct ccgccagcgt
     1441 catcagttgt tcaacctggg catcgatcag atcggcattc aacgcgcact cgctgaggat
     1501 gtcgcagcaa atcacgatat caccatcttc aacttccagc tcttctggat cggtcacttc
     1561 gtaaccgagt ttaaacgctt caactgccgc tttttccaga gtttccagat cgtctgcgga
     1621 aagatggtgt tcgatggtgt acagcgcgtc cggatcgctg ccatcttcca gtaattcttc
     1681 aataatcaaa cgtgtttctt cacgctgttc ttccagttgt tccgggtttg ccatggctca
     1741 ttcctcttaa tgtcctgccg atactcttat tgtcacacac cactgacatt gcctccacct
     1801 ttgtgataaa gatttatgct ttagacttgc aaatgaataa tcatccatat aaattgaatt
     1861 ttaattcatt gaggcgttag ccacaggagg gatctatgtc cgggttttat cataagcatt
     1921 tcctgaaatt actcgatttc acgccagctg aacttaacag cctgctgcag ttagccgcga
     1981 agctgaaagc tgataagaaa agcggtaaag aagaagccaa actcactggt aaaaacatcg
     2041 cgctcatctt cgaaaaagac tcgacccgta cccgatgctc tttcgaagtt gccgcatatg
     2101 accagggtgc tcgcgttact tatctcggcc caagcggtag tcagattggt cataaagagt
     2161 cgattaaaga cactgcccgc gtgcttggtc gcatgtatga cggtattcag tatcgcggct
     2221 atggtcagga gattgtcgaa acactggcgg aatacgctgg cgtgccggta tggaacggcc
     2281 tgaccaatga gtttcacccc acgcagttgc tggcggatct cctcaccatg caggagcatt
     2341 tacccggtaa agcattcaac gaaatgacgc tggtctatgc aggtgacgca cgtaacaaca
     2401 tgggcaattc gatgctcgaa gctgcggcgc ttaccggtct ggatttgcgt ctggtcgcgc
     2461 cacaggcatg ctggccagaa gctgcgctgg ttacggaatg ccgcgccctg gcacaacaaa
     2521 atggcgggaa tattacgctg acggaagatg tagctaaagg agttgaaggt gctgacttta
     2581 tctataccga tgtatgggtg tcgatgggtg aagcaaaaga aaaatgggct gaacgcattg
     2641 cattactgcg tgattatcag gtgaacagca agatgatgca gttgaccggt aacccggagg
     2701 tcaaattcct ccactgcctg cccgcgtttc atgacgacca gacgacgctt ggtaagaaaa
     2761 tggcggaaga gtttggccta catggcggaa tggaagtgac tgatgaggtc ttcgaatctg
     2821 ccgccagcat tgtttttgat caggcggaaa accgcatgca taccatcaaa gcggtgatgg
     2881 tcgcgacgct cagtaaatta aataattaat atctctataa agatgccggg aaatacaaaa
     2941 acatcccggc atcacattat tttatgcagt gatagttttt ttcttccagc caaatttttc
     3001 tat
//
LOCUS       DAZGAP010000336         3003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGAP010000336 DAZGAP010000000
VERSION     DAZGAP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.338"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..768
                     /gene="ompT"
                     /locus_tag="ACHLAM_005160"
     CDS             <1..768
                     /gene="ompT"
                     /locus_tag="ACHLAM_005160"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO7856527.1"
                     /translation="DWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDW
                     MDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWFLNEPNYRLGLMAGYQESRYSFTAR
                     GGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSG
                     WVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVTNKK
                     GDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1000..1668
                     /locus_tag="ACHLAM_005161"
     CDS             1000..1668
                     /locus_tag="ACHLAM_005161"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7856528.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1750..2092)
                     /locus_tag="ACHLAM_005162"
                     /pseudo
     CDS             complement(1750..2092)
                     /locus_tag="ACHLAM_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2105..2700
                     /locus_tag="ACHLAM_005163"
                     /pseudo
     CDS             2105..2700
                     /locus_tag="ACHLAM_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          908 a          675 c          648 g          772 t
ORIGIN      
        1 gactggaaat tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
       61 cagatatcta tcggggctgc tggctggaca acactcggca gccgaggtgg caatatggtc
      121 gatcaggact ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct
      181 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gttcctcaac
      241 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
      301 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
      361 ttcccgaatg gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc
      421 ttgactggaa gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc
      481 tgggtggaag catctgataa cgatgaacac tatgacccag gaaaaagaat cacttatcgc
      541 agtaaagtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaacg
      601 cctaatgcaa aagtttatat tgaaggcgca tggaatcggg ttacgaataa aaaaggtgat
      661 acttcacttt atgatcacaa tgataacact tctgactaca gcaaaaatgg tgcaggcata
      721 gaaaactata acttcatcac tactgctggt cttaagtaca ccttttaaca acgttaaata
      781 gattttctcc ccggtggtat tgtatagccc cggggaattt gtttaccgtt aaaagcaaac
      841 aatatcccct aatcttgtaa gcatttaacc tgcgtcctgg cgtgtaacct cttaccgcaa
      901 cacaagactc aatatattca aattcgttat gccgtggcat catttaaccc cttgtaattc
      961 atcgctataa ttgatttaat tcacaaataa aactataaca tggtgaaact aatgaaaaaa
     1021 aacacagatg atggggctaa aatttacaca ccacttaccc taaagcttta tgactggtgg
     1081 gttttgggtg tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca ccttcttcca
     1141 cactttctgg aacatttagg taacaaccat ctggatattg gcgttggaac tgggttttac
     1201 cttactcacg tacctgagag tagtctgata tctttaatgg atttgaacga agctagcctg
     1261 aacgcggcat cgacaagggc tggggaatca aaaattaaac ataaaattag ccatgatgtt
     1321 tttgatcctt atcccgcggc gttacatggt caatttgatt ccatttccat gttttacctt
     1381 cttcactgcc tgcctggaaa tatatctaca aaaagctgtg taatacgcaa tgcggcgcag
     1441 gccttaactg acgatggaac tctatacgga gccacaattc ttggtgacgg cgttgtgcac
     1501 aatagcttcg gtcaaaaact gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca
     1561 aaagattccg aagaaggctt aacacatata ctctcagagc atttcgagaa tgttaaaacc
     1621 aaggttaaag gtactgtagt aatgttttcc gcttcaggga aaaaatagca tccagccgca
     1681 gcacgttctt gcgcacgacg ttctgcggca taatcccaat gattactctc tgacagggtt
     1741 cacaggccac taaatatcag gggcagttga tgtatcaact cggttcagca acacccgata
     1801 catcttccag gcttccagca acgaggtttc ttcctccgta tcctttatcc atgatatgcc
     1861 gttccacttc tgaaactccc ctgccgggga tacctggtga cattttccgg taacggaccg
     1921 agttcagaaa taaataatgc gtcccctgat gccacgtcat aaaccgtttt accccgatgg
     1981 tcttcaacga gatgccacga tgcctcatca ctgttgaaaa caaccacgaa gccagccgga
     2041 atatctgggc aatatcagta ctgtttgcag gcaaacctgt atgaggtgga atggcacaag
     2101 agacttacaa aagaatcagg ccaaacgcct gattgatgag agccccgaac cactgaaatc
     2161 tgtcgtcaaa tttgcgctgg taactgttct gagaaagtcg aacatcataa atctggagtg
     2221 gcaacagatt gacatgcagc gacaagttgc cagggtgaat ccagaagata gcaaatcaaa
     2281 ctgcgccatt ggtgtggcgc tgaacgatac cgcctctaaa gtgttgcgtg atcaaacagg
     2341 caagcatcac aaatgtgggt gtacatacca gggcggctaa gcgagcagat ggaacatcaa
     2401 tgcctgcgct caggaagatg cgcattgaca gcaagacagc atggctatca gcttgtcgtc
     2461 gtgcaggaat tgaaaatttc cgtttccatg acctctggca tacctgggca agctggttga
     2521 ttccgtcagg cgtcccatta tcagtgcttc aggaaatggg cggatgggag tccagagaaa
     2581 tggttcgtag atatgctcac cttgcgccta atcatttgac tgagcacgcg aggaaaatag
     2641 acgacatttt tggtgataat gtcccactct ggaattatag aagaaataaa gaaggcgtaa
     2701 ctgattgaat tttaatggcg cgccctgcag gattcgaacc tgcggcccac gacttagaag
     2761 ttcctagaac gacattttaa gtcaacaact taccgcgcca tctctgcgct cacacgtccc
     2821 actacctcaa aacatgtaaa gccttgcaag ccattgcgag gccttatgtg tctcagtttt
     2881 gtccctcttt tttgtactaa aaaacatagt aattgaggat aaacctcatg ctattttcgc
     2941 ttatatgcct ctaaaggcat ggcacttaaa tagataaaag caccacaaaa gcataaaaaa
     3001 acc
//
LOCUS       DAZGAP010000337         2986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGAP010000337 DAZGAP010000000
VERSION     DAZGAP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.339"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..480
                     /gene="tssE"
                     /locus_tag="ACHLAM_005164"
     CDS             25..480
                     /gene="tssE"
                     /locus_tag="ACHLAM_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7856529.1"
                     /translation="MNRKSPSLYEILYGNFTGGLELNQVSEEEQVILSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTSILQGMPGTAHQLMRVLSDVLLKYEPRIKRVGVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            533..838
                     /locus_tag="ACHLAM_005165"
     CDS             533..838
                     /locus_tag="ACHLAM_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856530.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            893..2257
                     /locus_tag="ACHLAM_005166"
     CDS             893..2257
                     /locus_tag="ACHLAM_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO7856531.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILARHELKQSSKVALPE
                     AMVKGYITRLENQPVQGEVSSPVTLPAQALRSDAPDVQEHQPLPYSRLVYVVSETKTE
                     PTLFLQKSPLTFLKTFVTGVCTALLAVCVAIPGWQFLTQPSPAEQQLRALLAPLPDVL
                     SADQMAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPDN
                     PRVQEMVDNWHKSVRSRALPEEAMTGWNEGMARLQQLAERLNRLDEQRGKYMTVSELK
                     TEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLINRYQMENAGKP
                     KSQP"
     gene            2273..2905
                     /locus_tag="ACHLAM_005167"
     CDS             2273..2905
                     /locus_tag="ACHLAM_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO7856532.1"
                     /translation="MEMENTPGRARTPWTIEDVRYLETHYGVKKTREIAAYLNRSATA
                     VRAMAYLVGITTPVAADWSETEIEIIRRDYPAGVALADICARLNGRAPKSVCWMARRF
                     GLKRLGYWSAEEDEILRQYYPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRL
                     WTAEEWNVLSGNMASGLSALMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
BASE COUNT          784 a          697 c          829 g          676 t
ORIGIN      
        1 agtaatacaa attggattat tattatgaac cgaaaatccc catcattata tgaaattctc
       61 tatggcaatt tcactggcgg gctggagtta aatcaggtca gtgaggaaga gcaggttatt
      121 ctttcggtgc tcgataacat gcagcgtatt ctgaataccc gggctggaag ccttaaacat
      181 cttccggatt atggtctgcc ggatatgaca agcattctgc agggaatgcc gggaacggcg
      241 catcaactga tgcgtgtgct ttcagatgtt ctgctgaagt atgagccacg tattaagcgt
      301 gtcggggtga cgatgcagga gcagactcag cctggtgagt tgcattatgt cattgatgca
      361 gaacttaaag atgcaggact ggttcgttac ggaacaactt ttatgccgga gggcagggtg
      421 ttactgcgat atctgaagca acagcagttt gtggataatg tgatgacttt cagggggtga
      481 tttatggttc cccttatccg tgtgcttaac agacataaac gagggctgaa tgatgaaccc
      541 gttatttaat gacatccaga tgcgcctttt ttatctgaat catgctccgt attcctggca
      601 ctggaatgtc agattcaggc cacaggaagc cgtatatatt ggtaatgatg catgccatat
      661 taccattacc tgtaatcagt ccggttttca tttaaccagg gacgggcagc gactgttcac
      721 tgaacgttat atcagaacac tcagtgagtt attagcggtt ctgaagcggc ggtgggatgt
      781 gacaccggca atcatccgtg cggtggaata cctttcccgg gtaccggtat cacactgatg
      841 tcctttccgt aactgttaat catgatttaa tacagcaaca ccgaatctgc cgatgacttc
      901 tgaagtgaaa ctgaaaaccg gtggtgaccc ccggtcatta ccggattatg ctgccctgcg
      961 tgatgaaatc agcaaactga cccacccggc acgtccggat gtggactggc gttacgtgga
     1021 aacgctctgc ctgcgtctgt atgaacacaa tggcgtggaa ttacagacgg tatcctggta
     1081 caccattgcc cggatgcata cagcagggtt aagtggtctg aatgaagggc tggctctgat
     1141 tgtggcgctg acccggcatc actggtcagt gatgtggccg ctgaataccc acgcccggct
     1201 ggaaattatt accggtctgt tcagtcgtct gcaaaaaacg ctccgggcca tgccaccgga
     1261 tgagcgtgac aacctgcccc tgctgtatca gacagaaaca tgcctgaaaa cgctgagtga
     1321 catactggcc cggcatgaac ttaaacagag cagtaaagtg gctttaccgg aagccatggt
     1381 gaagggatat atcacccgtc tggagaatca gcctgtgcag ggtgaagtat cgtcgccggt
     1441 aacccttccg gctcaggcac tccggtctga tgctccggat gtacaggaac atcagcccct
     1501 gccgtactcc cgtctggtgt atgttgtttc tgaaacaaaa acagagccca cactcttcct
     1561 tcaaaaatcc ccgctcacgt ttttgaaaac gtttgttaca ggtgtctgta cggcattgct
     1621 ggcggtctgc gtggccattc cgggatggca gtttctgaca cagccatcac cggcggagca
     1681 gcaactcagg gcgctgctgg caccactacc ggatgtgctt tcagcagacc agatggcgca
     1741 actcagcaga acaccctcac tgctaaatca tacgtcagag tggataaccc tttccggtca
     1801 gcagataacg cgactgacag aactaccgcc ggcatacaac ctgcaacgca gtgcgcagct
     1861 gctgcagcaa ctgaggggac ttttccccga caatccccgt gttcaggaaa tggtggataa
     1921 ctggcataag agcgtaagga gccgcgcgct cccggaagag gcgatgacgg gctggaacga
     1981 aggcatggcc cgtttacagc aactggctga gcgcctgaac cgtctggatg aacagcgggg
     2041 aaaatacatg acggtcagtg aactgaaaac ggaggtgttt ggcatcatgc aggcttttaa
     2101 ccggcacata ccggcagaag agcagttacg tcgttatgat gaagcccgta accagaatgg
     2161 cagtgaacag cagcaaaaac aggctgaaat ggcgctgaat cagttgatta atcgttatca
     2221 gatggagaac gcaggcaaac cgaagagcca gccataatgg ctgagataaa acatggaaat
     2281 ggaaaatacc cccggaagag cgcgcacacc atggacaatc gaagatgttc gctaccttga
     2341 aacgcattat ggcgtgaaga aaacccgcga aatagcggca tacctgaaca gaagtgcgac
     2401 agcagttcgt gccatggcgt atctggtggg gataacaaca ccagtggctg cggactggtc
     2461 agagacagag attgagatta ttcgcaggga ctatccggca ggcgttgcgc ttgctgatat
     2521 ttgtgcccgt ctgaacggac gagcgccgaa atccgtgtgc tggatggccc gcaggtttgg
     2581 tctgaaacgc ctgggttact ggagtgcaga agaagatgaa atcctgcggc aatattatcc
     2641 ttcagaaggt gtgaaagtga gccgacgcct ttccggacgt acagaaaatg cagtgaaact
     2701 gagagcgcgg gagctgggtg taaagttcac gggaaacggg cgattacgtc tctggacggc
     2761 agaggagtgg aatgtgttgt ccggaaatat ggcatcgggg ctatcggcac tgatgaagtt
     2821 actgccgggg cgttcccgta gttcagttca gggggctatg aggcgtttac agaaaatgag
     2881 tgcgcagaaa tctgtcaggc agtagccaaa atattcctgc atcaggagaa cggcagtggc
     2941 aataataagc agcaaactga tacataatgc agcttctgta aacggt
//
LOCUS       DAZGAP010000338         2933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGAP010000338 DAZGAP010000000
VERSION     DAZGAP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.340"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..336
                     /locus_tag="ACHLAM_005168"
     CDS             <1..336
                     /locus_tag="ACHLAM_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7856533.1"
                     /translation="FSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMSVE
                     KLTLSGRQVNVLMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLYKG
                     VLLVRKGRL"
     gene            411..599
                     /locus_tag="ACHLAM_005169"
     CDS             411..599
                     /locus_tag="ACHLAM_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO7856534.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            743..1591
                     /gene="mscS"
                     /locus_tag="ACHLAM_005170"
     CDS             743..1591
                     /gene="mscS"
                     /locus_tag="ACHLAM_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO7856535.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIIGGIAARMISGVLNNLMLGRN
                     IDATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNL
                     AAGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIV
                     NFSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMN
                     FVVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSR
                     SVSSRQ"
     gene            1717..>2933
                     /locus_tag="ACHLAM_005171"
     CDS             1717..>2933
                     /locus_tag="ACHLAM_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7856536.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGI"
BASE COUNT          720 a          553 c          815 g          845 t
ORIGIN      
        1 ttttcttctc cggaagcgct ccgttttctc tggggagtgg ttgacagtcc ggtttatgat
       61 atcagacatc ttactgagcg atgttcagta aacgatatca gtcatctgta ccgcgcattc
      121 atgtcggtgg agaagctgac cctgtcaggc cggcaggtaa atgtactgat gatgctgctt
      181 tatggcagta acggaacaga gggggcacga tatcttggca tgtcagagaa aacattctcc
      241 actcataagc agaatctgat aaagaggttg cgggtacata atgaagttga gttgttgtac
      301 aagggggtgc ttctggtcag aaaagggagg ttgtgaagca gggcagctta tttccggtaa
      361 tgttaaacgc agcctctgat tcgttaattt cataatcata aggagttttt atgcgtctgt
      421 tgattgcttt tcttcttccc tggctgactt ttttcaccat tggccggcct ctgacgggga
      481 ttttttgtct ggtgttacag ataacgctgc tgggctggat accggcaaca atatgggccg
      541 tgtatgcgct ttcgcagtat aagacggaac agaaaatccg cgaagctacg gggaaatgat
      601 gactgcagaa gagcaggttg tcatagctgt gtatatgatg cgggataaag gcccggagat
      661 tcctgaaaat aaaatggttc gtggaatata aaacagggaa aaaacggatg tgatggacgc
      721 ttctggtgag gcggtttcat gaattgtccg taatcaggat atgctgcccg gttacgccgc
      781 gaatcttgtg gcagcagtgg ttattattat tatcgggggt atcgctgcac ggatgatttc
      841 tggtgtgctg aataatctga tgctgggcag gaatattgat gccacagtgg cgctgttcct
      901 ttccgcgctg atacgtcatg ccattatagc ctttacgctg attgctgccc tgggacgtgt
      961 gggggttcag acagcttccg taattgcagt cctgggggct gcaggtcttg ctgttggact
     1021 tgccctgcag gggtcattgt caaaccttgc agctggtatt cttctcgtga tgttccgtcc
     1081 tttcagggct ggtgaatatg ctgacctggg tggtgttgcc gggactgtgc agaatgtaca
     1141 tatattctca accacgatgc gtacactgga tggtaaaatt gttgtcattc cgaacgggaa
     1201 gattattgcc ggagagattg ttaatttctc cagggagcct gaacgacgaa atgagtttat
     1261 catcagtgtt tcatatgatg cagatattga ctgtgtgaaa caagtcctga cagatattgt
     1321 gatgtcagaa gagagggttc tgaagaacag ggaaatcact gttcgcctga atgagcctgg
     1381 ggagtcatcc atgaattttg tagtacgggt atggagcagg cgtgatgacc ttcagtctgt
     1441 atactgggat gtactggaga gaattaagcg ggagtttgat tcagaaggga taagttttcc
     1501 atatccacaa atggatatcc atcttgttcg ctccgggaag caggcagagt cggtaaccca
     1561 taacagtcgc tcagtcagtt ccagacagta aaatgaaaag cgcattttat ttgtgaaaga
     1621 tgaacagcct gaatctgtca ggctgttttc attgtgtttt tatggtcatt tttggtatat
     1681 gtactgtata taatgtcatc tgtacaagga tataaaatgc caaagaaaat aatgacaggg
     1741 attgatgttt ttgacgctgc catggagcgt attgaatggg tgtttgatac gttttcatca
     1801 gtttgtcttt ctttttccgg cggaaaagac tccactgtcc tgtttcacct gatggcgaat
     1861 attgcccgcc gaaaaaaacg ccgtttttct gtactgtttg ttgactggga ggctcagtat
     1921 cagtgtacca ttgagcatgt caccaggttg agggaaatgt acgatgatat cacagatact
     1981 tttttctggg tggcgctgcc tctgacgacc gttaatggag tgtctcaggt acagcctgag
     2041 tgggtatgtt gggaggacgg ggctgaatgg gttcgccagc caccagaaga tgccataacc
     2101 agagcgtctt tttttccttt ttatcagaat gcgatgacat ttgaagagtt tattccggca
     2161 tttaatcatt ggtttgcccg ggaaaaaggg atggttgctg cgattcttac tggtgttcgc
     2221 gctgatgagt cactgaatcg ttttatggga ctggtatccg gacgtaagct ccgttatgcg
     2281 gaggataaac cctggaccac ggcatcggct gagggatttt actacaccat gtatcctttg
     2341 tacgactgga aaacaagaga tgtatggatt taccatgcga gaacacgtgc tgcctataac
     2401 tctctttatg acctgatgta cagggctgga gttcctcttc gcaatatgcg ggtgtgtgag
     2461 ccttttggac cggagcaacg taaggggttg tggctttatc atgtactgga gccggataca
     2521 tggggcagaa tgtgtgctcg cgtatcaggt gctgtagggg gagcgctcta tgcaaatgaa
     2581 agtggtgctt attttgcttt gaggaaacag ataacaaaac cagttcacca tacatggcag
     2641 agttatgcaa tgtttctgct ggagtcaatg cctgagaaaa cggcggagca ttacaagaat
     2701 aagattgctg tttatcttcg gtggtggcag tctcatggat ggccggatgg catccctgat
     2761 gagcaggata atgaccaggg atgtaaagat attccctcct ggcgtcgcat ctgtaagacg
     2821 ttactgaaaa atgatttctg gtgtcggatg ctggcgttta gtccgaataa gccccgacat
     2881 tatgagcgct acctggccag aatgcgtgaa aaaaggaaag agtggggaat att
//
LOCUS       DAZGAP010000339         2854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGAP010000339 DAZGAP010000000
VERSION     DAZGAP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.341"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..1340
                     /gene="bluF"
                     /locus_tag="ACHLAM_005172"
     CDS             129..1340
                     /gene="bluF"
                     /locus_tag="ACHLAM_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO7856537.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            1555..2286
                     /gene="bluR"
                     /locus_tag="ACHLAM_005173"
     CDS             1555..2286
                     /gene="bluR"
                     /locus_tag="ACHLAM_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO7856538.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2507..>2854
                     /locus_tag="ACHLAM_005174"
     CDS             2507..>2854
                     /locus_tag="ACHLAM_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO7856539.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRVAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTKKNPAKIKEAALLHISGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYA"
BASE COUNT          822 a          610 c          621 g          801 t
ORIGIN      
        1 tcatcttcaa ctttttccat tttttatcaa tgtaaagaaa cagttatgtt ttggtattaa
       61 aagaaagaca ccggaagata tgtctgttac cgtcttactc tcgcctctcc cattaccctg
      121 gacagttaat gcttaccacc cttatttatc gtagccatat acgtgacgac gaacctgtca
      181 aaaaaatcga agaaatggtt tcgatagcaa atcgcaggaa catgcagtct gacgtaacag
      241 ggatcttact gtttaatggt tctcattttt tccagcttct ggaaggtccg gaagaacagg
      301 ttaaaatgat atatcgggct atatgccagg atccacggca ctataatatt gttgagctga
      361 tgtgcgatta cgcgcctgct cgccgttttg gcaaagcggg aatggaatta tttgatttgc
      421 gcctgcacga gcgagatgac gttttacagg ccgtattcga caaaggcaca tcaaaatttc
      481 agctaactta tgatgacaga gcgctacaat tttttcgtac ttttgtcctt gcaaccgaac
      541 aagcaaccta tttcgagatc cctgccgaag actcctggct ttttatcgct gacggatctg
      601 ataaagaact tgattcctgt accctttcac caaatataaa cgaccacttt gcctttcatc
      661 ctattgtcga tcccttatcg cggcggataa tcgcttttga agccattgtg caaaaaaatg
      721 aagacagccc gtcagctata gcggttgggc agcgtaaaga aggggaaatc tatacagcgg
      781 atctcaaaag taaggcgctt gcattcgcga tggcacacgc acttgacctc ggtgataaaa
      841 tgatttcaat caatctatta cctatgaccc tggttaacga acctgacgca gtctcttttt
      901 tacttgatga aataaaggcc aatgctctgg tgcctgaaca aatcatcgtt gaatttactg
      961 aaagtgaagt catatctcgg tttgatgagt ttgccgaagc ggttaaatcg ctaaaggctg
     1021 ctggtatcag tgtagcaatt gatcattttg gcgcaggttt tgccggtttg ttactcctgt
     1081 cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat gtccataaaa
     1141 gtgggccacg gcaggcaatt attcaggcga tcataaaatg ctgtacatca cttgaaattc
     1201 aagtctctgc tatgggtgtg acaacaccag aagagtggat gtggcttgaa tctgcaggaa
     1261 ttgagatgtt tcagggagat ctgtttgcga aagctaaatt gaatggtatc ccttcagttg
     1321 cgtggccaga gaaaaaataa ttttcagcac attatttcac atgatttcac atgatttccg
     1381 taaatcatcg tgaaaatgaa tacgttatgt ttaaatttaa tccacattaa aaataatgtt
     1441 tcattcaatt tagctgaaat cttatttata ctttgtacaa ttttagcgta tattgtacaa
     1501 agtattattg ggtcgtgtac aggcgacgga gatttgtgac cgcaaggagg aattgtggct
     1561 tattacagca ttggtgatgt tgctgaacgt tgcgggatta atcctgtcac tctccgggcc
     1621 tggcaacgcc gctacggttt gttaaaacca cagcgcagtg aaggcggaca ccgactcttt
     1681 gatgaagaag acatacaacg catcgaagag atcaagcgtt ggataagtaa tggcgttcct
     1741 gtaggcaaag ttaaagcatt actggaaacc accagccagg atacggaaga tgactggagt
     1801 cgcctgcaag aagagatgat gtcaattctt cgtatggcta atcctgccaa actacgcgcg
     1861 agaattattt cactgggtcg agagtaccca gtagatcaat tgattaatca tgtttacctt
     1921 cctgttcgcc agcgtctcgt gcttgatcac aacacctccc gcattatgag cagtatgttt
     1981 gacggcgcat taattgaata cgcagcagcc tcacttttcg aaatgcgccg taagcccggt
     2041 aaagaagcca ttctgatggc gtggaatgtt gaagagagag cacgattgtg gctggaagca
     2101 tggcgtttat cattgtcagg atggcacatt tctgttcttg ctgatcccat tgaatcgccg
     2161 cgcccggaac tgttcccgac gcaaacattg attgtctgga caggcatggc accaacgaga
     2221 aggcaaaacg aacttttaca acactggggt gagcaaggtt ataaagtcat ctttcatgcc
     2281 ccctaatttc acctttatcc cgccccttag acagaatctt ataatctgaa cccacaacag
     2341 cttttgtacc ggagctggtc cgttgcaaaa tgctcaccgg gagaattcag gtaatccccc
     2401 tttcacactt tgtacatatc acagccaaag gacagaccag attgaagtgg taaaaccgat
     2461 cgtgcacact acgcagtctc acaactaaca agaggttatt atcctgatgg atcaggtcgt
     2521 tgtttttcaa aagatgtttg agcaagtgag aaaagagcaa aacttctcct ggttttattc
     2581 agaattaaaa catcaccgcg ttgcacatta catttattat ctggctacgg ataacatcag
     2641 aattattact cacgatgaca cggttttgtt attaagagga accaggaacc tgttaaaagt
     2701 tagtacgaaa aagaaccctg ctaaaataaa agaggccgca ttgcttcata tttccggaaa
     2761 atctacattt cgggagtact gttcaacact ggcagacgcg ggcgttttcc ggtgggttac
     2821 tgatgttaat cataacaaac gcagttacta cgcc
//
LOCUS       DAZGAP010000340         2778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGAP010000340 DAZGAP010000000
VERSION     DAZGAP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.342"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..696)
                     /locus_tag="ACHLAM_005175"
     CDS             complement(<1..696)
                     /locus_tag="ACHLAM_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856540.1"
                     /translation="MTGLSEQAKELHMMMGRRKPPKIQAVLNTRYWKNTEKTIKRTVG
                     MNIHTLSQWCGEQYEKMFISKVAEVYMLHHTAAILRRKGVYVDVFKIPSKIMNPPEAR
                     ANCTGCPSSQNVSPFFAYSEPNTINFSESIRINYSYTLVPAYIRKKVSSLMGDSELRY
                     VYSFPGSLYSYLLEKHPLSEVKKLMEDLPSTEDENFRRSETGSQYLKGILGVKTRVSE
                     LFIRQFSLPFRFPH"
     gene            810..1694
                     /locus_tag="ACHLAM_005176"
     CDS             810..1694
                     /locus_tag="ACHLAM_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7856541.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICTTHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDHLF"
     gene            1813..2490
                     /locus_tag="ACHLAM_005177"
     CDS             1813..2490
                     /locus_tag="ACHLAM_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856542.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            2496..2734
                     /locus_tag="ACHLAM_005178"
                     /pseudo
     CDS             2496..2734
                     /locus_tag="ACHLAM_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
BASE COUNT          709 a          699 c          628 g          742 t
ORIGIN      
        1 gtggggaaat ctaaacggca aagaaaactg ccttataaaa agttcagata ctcttgtttt
       61 tacaccgaga atccccttca gatactggct acctgtttca ctcctcctga agttttcatc
      121 ttccgttgaa ggcaaatcct ccatcagttt tttaacttct gacaatggat gtttctcaag
      181 aagataagaa tataaactac cgggaaatga atacacataa cgaagttctg aatcccccat
      241 cagagatgag acttttttcc ttatataggc tggaactaga gtatatgaat aatttattct
      301 tatactttca gaaaaattta tggtgttagg ctctgaataa gcaaaaaaag gagagacatt
      361 ctgagaagaa gggcaaccag tacaattggc tctcgcttca ggcgggttca ttatttttga
      421 gggaatctta aaaacatcca cataaactcc tttacgcctc aatattgctg ccgtatggtg
      481 aagcatatag acttctgcca cttttgaaat aaacatcttc tcatattgtt ctccacacca
      541 ctgactgagc gtatgaatat tcatacctac agttcttttt atcgtttttt ctgtgttttt
      601 ccagtatcgg gtgttaagaa cagcctgtat cttcggcggt tttctccgcc ccatcatcat
      661 atgaagttct tttgcctgct cagataatcc tgtcatttat tacctccgaa tatattccat
      721 taaaaatgga aataattata cgcccatatt acctgaatga taaaccggat tactgtgatc
      781 cggttcagtg aaatcaacag gataccggta tgtcattcag ccattcttcc ctctctgcac
      841 aagtgaaatc atatctgacg tttcttcctg aagaaattcg ccagaaaatc cttgaacatc
      901 tccacggtgt tattcattac gagcccgtga ttggcattat gggtaaatcc ggcaccggca
      961 aaagcagtct gtgtaatgcc atttttcagt cccgtatctg caccacgcat cccctgaacg
     1021 gctgcacccg ccaggctcat cgcctcaccc tgcagattgg tgaacgcaga atgacgctgg
     1081 tcgatctgcc cggcattggt gaaacaccgc agcatgatca ggaataccgg gagctttacc
     1141 gtcagttact gccggaactg gatctgatta tctggatcct gcgggctgat gaacgtgcgt
     1201 atgctgccga tattgccatg catcagtttt tactgaatga aggtgcagat ccctcgcgtt
     1261 ttctgtttgt actcagtcat gccgatcgcg tgtttcctgc tgaagaatgg aacgacacag
     1321 aaaaatgccc gtcccgtcac caggaactct cactggcaac tgttacagtc cgggtggcaa
     1381 ccctgttccc gtcatcattt ccggtactcc ccgttgctgc gcctgtcggc tggaatcttc
     1441 cggcgtttgt gtcactaatg atccacgccc tgccaccgca ggcaaccagt gcggtttatt
     1501 cacatatccg gggggaaaac cgctctgaac aggcccggaa acacgcacaa cagacttttg
     1561 gtgaaaccat cgggaaaagt tttgacgtcg ccgttgcccg tttcagtttt ccggcctgga
     1621 tgttacagct tctgcgtaaa gcccgggacc gcattatccg cctgctggtc acactgtggg
     1681 atcacctgtt ctgagcgccc cggagtcccc cgtggcaacc gtataccttt acacgacaac
     1741 ggattacggt aaccactgcc gtataccgtc tgtcattatc aataacacac tctttaattt
     1801 cagaggtcag tcatgaatac cagaattctt tttcccctgc tgttcactgt ggcatccttc
     1861 tccgcctccg ccggtaactg ggctgtcaaa aacggctggt gccagaccat gacggaagat
     1921 ggtcaggcgc tggtaatgct gaaaaatggc acgattggta ttaccggcct gatgcaggga
     1981 tgcccgaatg gcgtacagac gctcctgggc agccgtatca gtattaacgg taacctgatc
     2041 cccacatcac aaatgtgtaa tcagcagacg ggattcaggg ctgttgaggt ggaaatcgga
     2101 caggcgccgg aaatggtcaa aaaagccgtt cactccatag cagagcgtga tgtgtccgtt
     2161 ttacaggcat ttggtgtacg aatggaattc acccgcggtg atatgctgaa ggtctgtccg
     2221 aaatttgtca catcacttgc cggtttttcc ccgaaacaga cgaccactat taataaagac
     2281 tccgtcctgc aggctgcccg gcaggcatac gcccgggaat atgacgagga aacaacagaa
     2341 accgctgatt ttggctctta cgaagtaaaa ggcaataagg ttgagtttga agtattcaat
     2401 cctgaagacc gtgcgtacga caaagtgacc gtcacggttg gtgctgacgg taatgccacc
     2461 agtgccagcg ttgaatttat cggaaaatag ccggtatgtc ggactgtcac cctgttttat
     2521 tgcccgaagg ccctttctca cgcaaacagg caatggctgc cacaacagct taccttaccg
     2581 caatgtgctt attgaagatg accatggaac gcatttcctg ctggttatcc gcaatgccga
     2641 agggcagcta cgctggcggt gctggaattt tgagtctgac gccggaaaac agcttaatgt
     2701 gtggctcgcc agtgaagggc ttctcagaca ataaaagtct tcatttcatc catcaggccg
     2761 cgtcttctcc gggagacg
//
LOCUS       DAZGAP010000341         2753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGAP010000341 DAZGAP010000000
VERSION     DAZGAP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.343"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..324
                     /locus_tag="ACHLAM_005179"
                     /pseudo
     CDS             64..324
                     /locus_tag="ACHLAM_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            306..392
                     /locus_tag="ACHLAM_005180"
                     /pseudo
     CDS             306..392
                     /locus_tag="ACHLAM_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            389..1540
                     /locus_tag="ACHLAM_005181"
     CDS             389..1540
                     /locus_tag="ACHLAM_005181"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO7856543.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            1547..2447
                     /locus_tag="ACHLAM_005182"
                     /pseudo
     CDS             1547..2447
                     /locus_tag="ACHLAM_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          826 a          607 c          671 g          649 t
ORIGIN      
        1 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
       61 ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc tgtcagaacg
      121 gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc gattatctct tcctgaaggc
      181 actttaggac aatgggttac cgccgccaga aaagggctcg gaactcctgg ttcccgcacg
      241 gtggctgaac tggaatctga aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt
      301 gagcgagata tattaaaaaa agcaactgta gattcaattg gtcaacgcaa cagttatgtg
      361 aaaacatggg gttgcggagg ttttttgaat gagacgaaca tttacagcag aggaaaaagc
      421 ctctgttttt gaactatgga agaacggaac aggcttcagt gaaatagcga atatcctggg
      481 ttcaaaaccc ggaacgatct tcactatgtt aagggatact ggcggcataa aaccccatga
      541 gcgtaagcgg gctgtagctc acctgacact gtctgagcgc gaggagatac gagctggttt
      601 gtcagccaaa atgagcattc gtgcgatagc tactgcgctg aatcgcagtc cttcgacgat
      661 ctcacgtgaa gttcagcgta atcggggcag acgctattac aaagctgttg atgctaataa
      721 ccgagccaac agaatggcga aaaggccaaa accgtgctta ctggatcaaa atttaccatt
      781 gcgaaagctt gttctggaaa agctggagat gaaatggtct ccagagcaaa tatcaggatg
      841 gttaaggcga acaaaaccac gtcaaaaaac gctgcgaata tcacctgaga caatttataa
      901 aacgctgtac tttcgtagcc gtgaagcgct acaccacctg aatatacagc atctgcgacg
      961 gtcgcatagc cttcgccatg gcaggcgtca tacccgcaaa ggcgaaagag gtacgattaa
     1021 catagtgaac ggaacaccaa ttcacgaacg ttcccgaaat atcgataaca gacgctctct
     1081 ggggcattgg gagggcgatt tagtctcagg tacaaaaaac tctcatatag ccacacttgt
     1141 agaccgaaaa tcacgttata cgatcatcct tagactcagg ggcaaagatt ctgtctcagt
     1201 aaatcaggct cttaccgaca aattcctgag tttaccgtca gaactcagaa aatcactgac
     1261 atgggacaga ggaatggaac tggccagaca tctagaattt actgtcagca ccggcgttaa
     1321 agtttacttc tgcgatcctc agagtccttg gcagcgggga acaaatgaga acacaaatgg
     1381 gttaattcgg cagtactttc ctaaaaagac atgtcttgcc caatatactc aacatgaact
     1441 agatctggtt gctgctcagc taaacaacag accgagaaag acactgaagt tcaaaacacc
     1501 gaaagagata attgaaaggg gtgttgcatt gacagattga atctacaaca gcgtattttg
     1561 cacaggagtc gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat ttcccattga
     1621 agcgatgtgt caggtatttg gtgtatccag gagcggttat tacaactggg tacagcatga
     1681 accctcagac agaaaacaaa gtgatgagcg gctaaaactg gagattaagg tggcacatat
     1741 ccgcactcgc gaaacatatg gaacccggcg gctccagacg gagctggcag agaatggcat
     1801 catcgttggt cgtgaccgac tggcacgtct tcgtaaggag ctaaggctac gctgtaagca
     1861 gaaacgcaag ttcagagcga ctacgaactc gaaccacaat ctgccagttg cgccaaatct
     1921 gctgaaccag acgttcgctc ctacagcacc aaatcaggtc tgggtggcgg acctgacgta
     1981 tgttgccaca caggagggat ggttgtacct cgctggcatc aaagatgttt atacgtgcga
     2041 aattgtcggc tacgccatgg gagagcgcat gacaaaagag ctgacaggta aagccctgtt
     2101 tatggcgctc aggagccagc gcccacctgc cgggcgggct aatccaccac tctgagcgag
     2161 gttcacagta ctgcgcatac gattaccggg tcatacagga gcagtttggt ctgaaaacat
     2221 caatgtcgcg taaaggtaac tgttacgaca acgctccgat ggaaagcttc tggggaacgc
     2281 tgaaaaatga gagcctgagc cactatcgtt ttaataaccg ggatgaagcc atctcagtaa
     2341 tacgggaata cattgagatt ttctacaatc gtcagcgtcg tcactctcgt ctggggaata
     2401 tctccccggc agccttcagg gaaaaatatc atcagatggc tgcttaaaaa aagaacaaat
     2461 ggtagtgtcc gctattgcca gtacacctca gtatagtggt atgcgtgaac atatatagtg
     2521 gcattttgtc tgatgttgtt aaggtttttt agcaggtcta cagtcaactc catcgcggta
     2581 tccttcaggt tgttatcatt cgttgatgat gcgatctgtt gaaggcgttg gcgacaatta
     2641 attctttttc tcttctgaaa aatttacgga atacaaaaaa gatctgtttt taactgattg
     2701 ttactttctg aagatattct ctgctccatt tgttgaagcc ggagagtgtt gtt
//
LOCUS       DAZGAP010000342         2727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGAP010000342 DAZGAP010000000
VERSION     DAZGAP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.344"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            90..2369
                     /gene="ydeP"
                     /locus_tag="ACHLAM_005183"
     CDS             90..2369
                     /gene="ydeP"
                     /locus_tag="ACHLAM_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO7856544.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2617..>2727
                     /gene="safA"
                     /locus_tag="ACHLAM_005184"
     CDS             2617..>2727
                     /gene="safA"
                     /locus_tag="ACHLAM_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO7856545.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIF"
BASE COUNT          734 a          643 c          660 g          690 t
ORIGIN      
        1 atatccatgt tgacgataat ccctttgtat atactgtgca tcatcgctat tacaaatcct
       61 aataattcat ttccacacag gataagcaga tgaagaaaaa aattgaatcc taccagggag
      121 ctgccggtgg ttggggtgct gttaaatccg tagcaaatgc agtacgtaag cagatggata
      181 tacgccagga tgttattgcc atgtttgata tgaataagcc agagggcttt gactgtccgg
      241 gatgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa aacggcgcaa
      301 aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt gctgagaata
      361 cggttcaatc attacttacc tggggagatc acgagcttga ggctgcggga cgtctcactc
      421 agcctttgaa atatgatgac gtcagcgact gttacaagcc attaagctgg caacaagctt
      481 tcgacgaaat tggcgaacgc cttcaaagct atagtgatcc caatcaggtt gaattctata
      541 cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc cgtgaatacg
      601 ggagcaataa cttccccgac tgctccaaca tgtgccatga accgaccagt gtgggtttgg
      661 cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag aaatgcgatt
      721 tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgttg acttcgttgc
      781 gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc
      841 tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt
      901 tggccagtgc ctactacaac gtgcgcattg gtggtgatat ggcgttgctc aaggggatga
      961 tgcgcctgtt aattgagcgc gatgatgctg caagcgccgc aggtcggccc tcattgctgg
     1021 atgacgaatt tattcaaacg cataccatcg gctttgacga gctacgccgt gacgtgctca
     1081 attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac
     1141 ttgctgatgc atatgccgct gccgaacgaa ccattatctg ttacggaatg gggatcactc
     1201 agcacgaaca tggtacccag aacgtacagc aactggtcaa tctgctgttg atgaaaggta
     1261 acattggtaa gcctggtgcg ggtatctgcc cactacgagg tcactctaat gtacagggcg
     1321 accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc ctgggtgagc
     1381 gctatggttt caccccacct catgcacctg gacatgctgc aattgccagc atgcaagcaa
     1441 tatgtacggg gcaggctcga gcattgatct gcatgggggg caactttgca ctggcaatgc
     1501 cagatcggga agcaagcgct gtaccgttaa cacaattaga tctgacggta cacgtagcca
     1561 ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg ccggttctgg
     1621 gacgcagcga gattgacatg caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa
     1681 tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg ctgaaatcag
     1741 agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg gtagcctggg
     1801 agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt
     1861 tcgccgacta taaccagcgc atccgtcatc ccggtggttt tcacctgata aatgcagctg
     1921 ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagt aaagggctgt
     1981 tagaagatcc ctcttcagcg tttaacagta aactggtcat ggcgacagta cgcagccacg
     2041 atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggtgta ttcggtcaac
     2101 gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa
     2161 gagttaatct tattgcgctc acgccagacg gtaaacgtag ctcacgccgc atggatagat
     2221 taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt ccagaatcga
     2281 atcacatgct aacacttgat aaccacgatc cattaagtgg cattcctggc tataaaagta
     2341 ttccggttga attagaacca tcaaattaat tcgcctcctc atttcttctg ctgtcatccg
     2401 cacagcagaa gaattcctca ttgaccattg tcgcgcaatt tactcacatg tgctaaatta
     2461 aactgctcgt tataagatat atacctccgc ctacagctgt aagaaactcc gctcagtact
     2521 gaagcgccag tcctatttcc cctattctcc agcctgttat attaagcata ctgattaacg
     2581 atttttaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac agtgaaaaac
     2641 aaaatcacgc aaagagacaa ctataaagaa atcatgtctg taattgtggt tgtcttatta
     2701 ctgacactta cgttgatagc cattttt
//
LOCUS       DAZGAP010000343         2694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGAP010000343 DAZGAP010000000
VERSION     DAZGAP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.345"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(738..2675)
                     /gene="iolC"
                     /locus_tag="ACHLAM_005185"
     CDS             complement(738..2675)
                     /gene="iolC"
                     /locus_tag="ACHLAM_005185"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFO7856546.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVLDIDYRPVLWGLTSLGDGETRFIASPQVTEQLQQVLHHFDLIVG
                     TEEEFHIAGGSTDTLTALRRVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGV
                     RVDILNVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQEL
                     DDYLSREHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEF
                     TIPELKMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGS
                     YPLKLEHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHD
                     LLLEVILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDP
                     ECRGILILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLI
                     AAVKQKYRTLIDYWREYRPAL"
BASE COUNT          694 a          673 c          697 g          630 t
ORIGIN      
        1 gttaaagtat gttgtagaga aaagtttgtc tgaagtggcc tactgaaatg aattgcgcag
       61 gaaagtgaga cggaagatcg gtcagaccga caccagcaaa atatgatata tccgacgacc
      121 atcaaaggtc ataaaggcga taaggaatag gtgcgcagat atccgtcatg gctctgcaaa
      181 aaactgtaaa atgaagccga atgtacgggg gcagtctgaa ccccaactga agcatctttt
      241 ttgcacaaaa tcgggagtcc atgtacggat ataaagctgc aacctaccgt caatgtcaga
      301 acaatggaag ctgtgaaaaa cgggatgcgt taatataaac tccatgacag tagtggaacg
      361 ggttaatcaa gcaggattac cagcaagatg agggagctga ttcagcacag gcgacctgac
      421 cctggccacg gataaccgtc tgtcgcccgt ctgaggtgtc gttttttcgt tctgccaccc
      481 ctcggttgag taaaatggaa attaaccgtc aagctgcctg tagcgtaccg atcagaagat
      541 tgctcagatg gtacagctgt tgtacgttct gcggcaacgt gacgagggaa ctaccaatag
      601 ccagacagtt cagaaatatc attaatgcag atacgaaaca gcactgtcgg gatgatgagg
      661 aaagtaggac ggtcatgaaa catctttgaa tacgccccga gcaagaatac agacgcggga
      721 cgtaatgcag acggatacta aagcgccgga cgatattcgc gccagtagtc gatcaatgtg
      781 cggtattttt gtttcactgc tgcgatgagc tgttcatcgt taatctggcc gctgaaccac
      841 tgccgtgatg gctcgccaaa gatggtgcga cctacggcga agcctttaac ccatggtata
      901 tccgcagcgg cggcaaagcc agatttcagc tcgctttccg gtgcatccag tccaagtatt
      961 aaaataccgc ggcattcggg atcgtagcgc tcaatatgct ggctgatttc ttgccaacga
     1021 gccggactta acggtggaag cttccaccag tcaggctgga tccccagcag gtaaaaacgt
     1081 tccacaatct gtgtatagta atgctcgctt tgatctgctg cgtcgcgtgg caagatgacc
     1141 tccagcagca gatcgtgtcc agactggcag caggcccggt atatctcatc aatgagtgct
     1201 tcttgcttaa ggcggaggga ttctgcatcc agtgggtgat agaagacaag gcatttcacg
     1261 acgtgctctt tcggccaact gacaagttgt gaaccgatat taccgtgctc cagcttcaga
     1321 ggatatgacc ccggcatttc gataggacgt ccaatccacc atccctgacc agtcacgtcg
     1381 ttaagtgctt tctgaccaaa agtagtatcc gccaggatgc cgcagttatg cagtaatcca
     1441 gcctcgtgcg ccgcctgacg tgcgccttcc agtagtagca tcttcagctc agggatagtg
     1501 aactcgctgg cacctgtcgc tctggcgata tccaccagtt gattgcgatg gtcaaaggcg
     1561 aatacacaca gttccggcca gtgctgtttt cgggttgtcg ttcggtgaag atggttaagg
     1621 tgagggtctt tgtcgggtct ggtgatagcg tgctcacggg agagatagtc atccagttcc
     1681 tgtttggttg gcatcgcggg ggcgcagccg tggcgcgata caactaatgc gccacatgcg
     1741 ttagcataac gacaagcttg ctcccaacct tcatcgttca gatagcctcg aagcagtccc
     1801 gacataaagg cgtctcctgc acccagcaca tttaggatgt ccacacgtac accgctgtgc
     1861 atctgcacct gagaccagtc gtcggcgatg tttccttcaa atactgaaca ccctaatgcg
     1921 ccgcatttgc ataccaatac cgcagaggtt agctgacgca cacggcgcag ggcggtcaac
     1981 gtatcagtac tgccgccggc gatatgaaat tcctcttcgg tgccaactat cagatcaaaa
     2041 tggtgcaata cctgttgaag ctgttcagtc acctgtggtg aagcgatgaa acgtgtttca
     2101 ccatccccca gcgatgtcaa cccccatagt accggacgat aatcaatatc cagcactcta
     2161 cgcagaccgt gtttttgagc ataggtcagc gctttaagca ctgcggcgcg ggtattgatg
     2221 tgagaaagat gagtgccggt gacggccagg gcacgtgaag aggcaatgaa tgtttcgtgg
     2281 atatcctcag gcgccagcgc catatccgca cagttatctc gataaaagat cagcgggaag
     2341 gtgtcctgat ctttaatacc aaggatcacc agggctgtca gacgttcagg atcagtcaac
     2401 aggcattgtg tattgacgcc aacacggttt aattcttcgc ggatgaaacg tcccatatgt
     2461 tcatcgccca cacgtgccag catggcggaa cgtagtccct gaattgctgt gccgtaagcg
     2521 acgttgcctg atgagcctcc taaatatttt gcaaaggttg tcatgtcttc cagacgtgac
     2581 ccgatttgtt gaccatataa atcaacggcg acacgcccca gacagataat gtccagcgtt
     2641 ttttgctcac tgctgactgt gttgttcata gagatatcct cctacagaag accg
//
LOCUS       DAZGAP010000344         2682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGAP010000344 DAZGAP010000000
VERSION     DAZGAP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.346"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(40..>2682)
                     /gene="chiA"
                     /locus_tag="ACHLAM_005186"
     CDS             complement(40..>2682)
                     /gene="chiA"
                     /locus_tag="ACHLAM_005186"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO7856547.1"
                     /translation="PALAMEVWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKAKAN
                     DATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQGTV
                     PSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRYVRAAT
                     ATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQEGS
                     STWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQYGNPGSCSV
                     KPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGSEIYHV
                     IFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTATAAEMSQYGNPTTCEIDNGGV
                     IVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSASTV
                     YVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNEELNNA
                     PQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPKKA
                     WPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQNY
                     AQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVLDFDI
                     EGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDAKA
                     KGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLHPNKSDAEI
                     DAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTNLSPE
                     FHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          621 a          710 c          642 g          709 t
ORIGIN      
        1 caaaaaatgc ggcgataccg gaaggtatcg ccaacacatt tattgcttag taaacggcgc
       61 gaagatttcg ctaaatgcgt acttcggtgc ctgttcttta gtcaggccgt ggaattccgg
      121 agacaggtta gtgccgcctg gcaggtcgcg cgcgattgac cagataccaa ccataccgag
      181 attacgcttc tgcgcatcct gcatgaccag acgagcatca gagagataga acacctcgcc
      241 ctgaacgtcg ttcacgccaa ccatcggcgt ggtacccatc atagcgtcaa tttctgcatc
      301 gctcttattg ggatggaggc ctttcaattg tgaatgcagg ttggcaatcg cagacgttgc
      361 acacttaccg tgaatgttct ggccttcggt atttgcagac tgacaaatcg cgttaccgta
      421 gtccattgtc atcacgttca cacccgccag ctcaacacct ttcgctttgg catcgctcag
      481 gacattcatc ccttccggcg tcaggccagt cggcagaatt ggcaaggtgt accagatagc
      541 aatatctttg ccctctgact tccatttatc ctgcactttc ttcacagcaa ggttacgacg
      601 ttcaatagat gcctgatccg caacccaggt gccttcgata tcgaagtcca ggactctgag
      661 gttcaggtta tcaacgatgt cataataatg ctgcatcaga tcgtctacgt tcttacagga
      721 agcagccagc ggagcgttgt tagcaccacc gatagacagc atcacatcgc cgccagcttc
      781 acgcagagct ttgattttgc tgtactgagc gtaattctgc ataccgtaag cggtacccca
      841 tgtcggcaga caggtgttcg catctttact caccacaaac gccagcgtga agtggttgac
      901 gttatgattc ttagccagcg cagccagatc cgggatcgta ttcagcgtaa agtcaacata
      961 cggtgcataa acgtgtttcg gccacgcttt cttcggcgta cctacacgtt ctttggttcc
     1021 ggtccagtca acaaaaacac gccacgggtt gctgtcagaa ggagaaactt tctgcacttt
     1081 actctgagaa atgtagttcg caccgttaaa gcgaatcagc gtatcgctgg cataaagcgt
     1141 ttctggatta aactgcggcg cattattcag ctcttcgttg ctatagctct gagccttacc
     1201 taacggcttc cacgggcggc tattgctacc ggtggcgttt tggttcgcaa ccagagctgg
     1261 gttgtcgctt tgcgtccaga acagcgcttc ataagcctgc ccatcaacaa caacgcgatc
     1321 acctttcacg tacacggtgg aagcggacca tgctttcgcc ggttcgtatt tcttccacgg
     1381 gttatcgccg cccgggacaa agccccatgc gtcttgatcg acagaggttt tataatgtgc
     1441 atcgttataa tctacgatgc tgttcgcgct gtacgctttg ctggcctgga aaccatccgc
     1501 aacaatgacg ccgccattat caatttcaca ggtagttggg ttaccgtact gactcatttc
     1561 cgcagcggta gctgtacgca cgagacgcca tgggttattg gagttttctg cactagtgcc
     1621 acgtgggcaa tcttcagaac caacccacca ggcattctga taaacattac cattgaagat
     1681 aacatggtaa atttcgctac cttcctggcc actccacgcc tgcagtgagt aatcgttact
     1741 gctatccgct ggtgttgatg gctcgttatt atccggagta ggagtaactg gagttaccgg
     1801 tgtttccgga gttggatcaa ccggagtcac agcaccgcta ttattatccg gcttaacgga
     1861 acaggaaccc ggattaccat actggctgat ttctgttgcc gttgccgcgc gaacataacg
     1921 ccatgggttg ctggcatcgt tttctttcgc atcgcctggg caatttgccg cgcctaccca
     1981 ccaggcgttc ttataaatgc cgccgttgta gataacgtac catgtagaag aaccttcctg
     2041 acctttccag gctacgacag catagttgtc gtttgactta tcagctggag ctggctgaac
     2101 atcaggagcc ggtttaacgt ccggtgccgg tttcacatcc ggcgcaggct gtggtgctga
     2161 agtacaggac tgtggattac tggtttctga gatttccgtt gccgtagcgg cacgaacata
     2221 acgccatggg ttgctggcat cattgccttt cgcatcaccc gggcaattag aagaggctac
     2281 ccaccaggca tttttatata ccgcaccatt aaagacgaca taccaggtct gaccgccctg
     2341 ctgtttattc caggcaacta cagaagaatt agacggaaca gtgccctgtg ctggtgtagc
     2401 cgaaccgcca ttagccggcg tattggatgc aggcgtattt gaatttgaag aagatgagct
     2461 gccgctcttt tcgcaggaaa gtgtattgcc aaactgacta atttcagcag ctgtcgcggt
     2521 acgctttaaa cgccatgggt tagttgcatc atttgctttt gcttttcccg ggcaatttgt
     2581 agaagaaacc caccaggcat tttcataaat tttgccatcg aaaataacct gatatttatt
     2641 accaccttgt tggttattcc atacttccat tgccaatgct gg
//
LOCUS       DAZGAP010000345         2663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGAP010000345 DAZGAP010000000
VERSION     DAZGAP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.347"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..1117
                     /locus_tag="ACHLAM_005187"
     CDS             65..1117
                     /locus_tag="ACHLAM_005187"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO7856548.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1193..2626
                     /locus_tag="ACHLAM_005188"
     CDS             1193..2626
                     /locus_tag="ACHLAM_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO7856549.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT          633 a          592 c          687 g          751 t
ORIGIN      
        1 agatgctttg cccttaatgt aaccatatcg caataagtta tgtttttaaa ttgagggcat
       61 tattatgaaa aaaataccct gcgtgatgat gcgaggtgga acctcgaggg gcgcgttcct
      121 gttagcggaa catctacccg aagatcaaac gcagcgcgat aaaatattga tggcaattat
      181 gggttccggt aacgatctgg aaattgacgg tattggcggc ggtaatccgc tgaccagtaa
      241 agtcgccatt attagccgct ccagcgatcc gcgtgcagat gtcgattatc tgtttgctca
      301 agttatcgtc catgagcaac gtgtcgatac cacgcctaac tgcggcaata tgctgtctgg
      361 cgttggggca ttcgccattg aaaatggttt gattgcagcg acttcgccag ttacccgcgt
      421 acgtatccgc aacgtcaata cgggtacgtt tatcgaagct gatgtgcaaa cgccaaatgg
      481 tgttgtcgag tacgagggta gcgccagaat tgacggcgta ccgggtactg ccgcaccggt
      541 tgcgctcact ttcctgaatg ccgccggaac gaaaaccgga aaagtcttcc cgacggataa
      601 tcagattgat tattttgacg atgtcccggt gacctgtatc gatatggcga tgccagttgt
      661 cattattcct gctgaatatc tgggaaaaac aggctatgaa ttaccggcag agctggatgc
      721 cgacaaagca ttattagcca gcattgaatc tatccgtcta caagcgggta aagcaatggg
      781 cttaggcgat gtcagtaata tggttatccc taaacctgta cttatttctc cagcgcagaa
      841 aggcggggca attaatgtgc gttattttat gccgcattct tgccatcgcg ctttggcgat
      901 aaccggtgct attgctattt cctgtagttg tgcattggaa ggcaccgtca cccgacaaat
      961 cgtcccttct gtaggatacg gcaatatcaa tattgaacac cccagtggtg cgctcgacgt
     1021 tcatttaagt aatgaaggtc aggatgccac gacgttacgc gcatctgtta ttcggacgac
     1081 aagaaaaata ttttccggtg aagtttatct tccctgaaaa attttgttgt cgtgataatg
     1141 acaatcaata aaggaattct gtatgagtca tacagaaaga acaggatatt aaatgaataa
     1201 aaaatcgtta tggaagctaa ttctgatatt agcgatccca tgtattattg gctttatgcc
     1261 agctccggca ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat
     1321 tgtggggctg gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgtcgccgc
     1381 atcgatggta gtggtgggta acttatcagg tggggaattt aaaaccaccg ccgtattaag
     1441 cggttactct tccggtacca cctggctggt gttttctgcg tttactttaa gcgccgcgtt
     1501 tgtaaccaca ggcttaggta aacgtattgc ctatctgctg attggtaaaa ttggtagcac
     1561 taccctgggt ctgggttacg ttacggtatt tctcgatctg gtactggctc cggcaacacc
     1621 gtctaacacc gcgcgtgcgg gcggcatcgt gttaccgatc atcaacagcg tggcagtggc
     1681 tttgggatca gaaccggaaa aaagtccgcg tcgtgttgga cattacctga tgatgtccat
     1741 ttacatggtc actaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct
     1801 ggcgctgaaa atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggcgct
     1861 ggccgctgga ttgccgggta tcattatgct gctggtcacc ccgctggtga tttacaccat
     1921 gtatccgcca gaaattaaga aggtggataa caaaaccatc gccaaagcgg gccttgccga
     1981 actgggaccg atgaaaatcc gcgaaaaaat gctgctcggt gtcttcgtgc tggcgctgct
     2041 gggctggatt ttcagtaagt cactgggggt tgatgaatcc accgtggcaa tcgttgttat
     2101 ggcgactatg ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg
     2161 ctggaatacc ttaatctggt acggcggtat tatcggctta agctccttat tatcgaaagt
     2221 taaattcttt gaatggttag ctgaagtctt taaaaataac ctggcatttg atggtcacgg
     2281 taacgttgct ttcttcgtta ttattttcct cagcatcatc gtgcgttatt tcttcgcttc
     2341 cggtagtgcc tatatcgttg ctatgttacc tgtatttgcc atgctggcga acgtctccgg
     2401 cgcaccgtta atgttaaccg cgctggcact gttgttctct aactcctatg gcggcatggt
     2461 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
     2521 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcctggtgc atatcaccct
     2581 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
     2641 cttcaagttg catgtgctgc gtc
//
LOCUS       DAZGAP010000346         2656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGAP010000346 DAZGAP010000000
VERSION     DAZGAP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.348"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..1706
                     /gene="mppA"
                     /locus_tag="ACHLAM_005189"
     CDS             93..1706
                     /gene="mppA"
                     /locus_tag="ACHLAM_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO7856550.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPVYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1757..>2656)
                     /gene="ynaI"
                     /locus_tag="ACHLAM_005190"
     CDS             complement(1757..>2656)
                     /gene="ynaI"
                     /locus_tag="ACHLAM_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO7856551.1"
                     /translation="VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAV
                     ILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLL
                     TFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRIT
                     KITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREML
                     KNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSH
                     GADFAFPSQTLYMDNITPPDQGR"
BASE COUNT          776 a          589 c          629 g          662 t
ORIGIN      
        1 ctttgtcact tttttgatgg aagttgtttg catttcttta aggcgaaaca aataattacg
       61 catcaatttt aatgtcggtt agagggaaac ttatgaagca ctctgtttca gtcacgtgtt
      121 gtgcgctgtt ggtcagcagc atttctcttt cgtatgctgc tgaagttccg agcggcacag
      181 tactggcaga gaagcaggag ctggtgcgcc acattaaaga tgagcctgcg tcgctggatc
      241 ccgctaaagc cgtgggcctg ccagagattc aggtcattcg cgatctgttt gaaggactgg
      301 tgaatcagaa cgagaaaggg gagattgtcc ccggcgttgc gactcagtgg aaaagtaatg
      361 acaaccgtat ctggactttt accctgcgcg ataacgcaaa atgggcggat ggcacaccgg
      421 taacggcgca agattttgtc tacagctggc aacgtctggt ggacccaaaa acattgtcgc
      481 catttgcatg gtttgccgcg ctggcgggaa tcaacaacgc acaggcgatt attgatggta
      541 aagccgcgcc tgaccagctt ggcgtcaccg cagttgatgc ccaaactttg aaaatccagc
      601 ttgataaacc gttgccgtgg tttgtgaatt taaccgctaa ctttgccttc ttcccggtgc
      661 aaaaagccaa cgtagaaagc agtaaagagt ggacgaaacc cggaaatctg atcggtaatg
      721 gcgcttatgt tcttaaagag cgcgtagtca atgaaaaact ggtcgtggta ccgaataccc
      781 attattggga taacgccaaa acggtgctgc aaaaagtgac cttcctgcca attaatcagg
      841 aatccgcagc cactaagcgt tatctcgcgg gggatattga tatcaccgaa tccttcccga
      901 aaaatatgta tcagaagctg ttgaaggata ttccggggca ggtttatacg ccgccacagc
      961 tcgggaccta ttattatgcg tttaacacgc aaaaagggcc gacggccgat cagcgtgttc
     1021 gcctggcatt aagtatgacg atagatcgcc gcctgatgac cgaaaaagtg ttgggaacgg
     1081 gcgaaaagcc agcgtggcat tttacaccag atgttactgc aggatttacg ccggaacctt
     1141 cgccgtttga acaaatgagt caggaagaac tgaatgcgca ggcaaaaact ttgttgagcg
     1201 cagctggtta tggtccgcaa aaaccgctga agctgacgct tttgtataac acttcagaaa
     1261 accatcaaaa aattgcgatt gctgtagcat caatgtggaa aaagaacctt ggcgtagatg
     1321 ttaaattgca aaatcaggaa tggaaaacct atatcgatag ccgtaacacc ggcaattttg
     1381 atgttatccg agcctcgtgg gtaggggatt ataatgaacc ctccactttc ctgacattat
     1441 taacgtcaac gcattcagga aatatttcac gctttaacaa tccggtatac gacaaagtac
     1501 tggcccaggc atcaacggaa aataccgtta aagcgcgtaa tgccgattac aatacggcag
     1561 aaaaaatcct catggagcaa gcaccgattg caccaattta tcaatatacc aatggacgat
     1621 taatcaagcc gtggctgaaa ggttatccca ttaataaccc ggaagatgtg gcgtacagtc
     1681 ggactatgta tattgtgaag cattgatgtg atgggaactg gcgttaccct tgtggataac
     1741 gccagtgatg tctgatttag cgaccctggt ccggcggtgt aatgttatcc atatacagcg
     1801 tctggctggg gaaggcaaag tccgcgccgt gtgactgtac aatatcgata atcttcaaat
     1861 aaacgtcttg ctgtgcagca agccattcag cccataccgt ggttttggta aagcaataaa
     1921 ccataatatt caatgaagag tcagcaaact ggttgaaata aaccagtaag gtttgtcgct
     1981 ggtcgatggc cgggtgattt ttcagcatct cacgtacggc ttcgacaata acgcccactt
     2041 ttgccgcatc ctcataacgt aaaccaatgg tcgtggtaat gcgccggttg gtcattcgtc
     2101 ctgggttttc tacgctgatc gacgaaaaca gcgagttcgg tacgtacaac ggacgattat
     2161 caaaagtcgt aattttggta attcgccagc caatttccgc tactgtacct tcgatatttc
     2221 tgtccggtga acggatccag tcgccaatac taaaaggacg gtcgaaatag agcataatcc
     2281 cggaaaagaa gttactcaaa atatctttac cggccatacc gacagccaga ccaccaatac
     2341 caccaaaggt caacaagcca gaaaggctca taccgaaatg ttcaccataa agcagaacaa
     2401 gcaccacaat aatggtgatt ttgatgatac gcgacataat ccgcgcactg gtgatatcgc
     2461 gacctttttt aatctgctgt ttttcaaact gatttatcag cagaaatagc ttaatcgtca
     2521 gaataaccgc aatcagggac gtacagataa aatcgataac gcctggggtg ataaatttga
     2581 gtttatagtt ttctataaca taattaatga tgctaccaac agcactgata attatggtgt
     2641 agattaaaaa ttgcac
//
LOCUS       DAZGAP010000347         2653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGAP010000347 DAZGAP010000000
VERSION     DAZGAP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.349"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..624
                     /gene="glmS"
                     /locus_tag="ACHLAM_005191"
     CDS             175..624
                     /gene="glmS"
                     /locus_tag="ACHLAM_005191"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO7856552.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            624..2012
                     /gene="glmL"
                     /locus_tag="ACHLAM_005192"
     CDS             624..2012
                     /gene="glmL"
                     /locus_tag="ACHLAM_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO7856553.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQSYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSASANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            2031..2516
                     /locus_tag="ACHLAM_005193"
                     /pseudo
     CDS             2031..2516
                     /locus_tag="ACHLAM_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
BASE COUNT          726 a          655 c          662 g          610 t
ORIGIN      
        1 atttgaaaac acactttaaa caatcatttc ccgaaagaca ttttgaaaat agatcaattg
       61 caacattacg gtcagataaa gacactcctg tgatattaaa cctgtgccgt tatgagcggc
      121 cataaatacg acgtgtgtaa cttattttac tctctgtctt acaaaggatt acccatgaag
      181 aaagcaacac ttgtgattgg cgttattggc gcggactgcc atgcagtagg caataaagtt
      241 ctggaccgcg tttttagcaa ccatgacttc agagttatta atctgggcgt aatggtcagc
      301 caggacgaat atattgatgc tgccattgaa actggtgctg acgcgattgt cgtctcctct
      361 atctatgggc atggcgatat cgactgtctg ggcatgcgcg aacgctgcat cgagcgtgga
      421 ctgggggaca tcctgctcta tgtgggcggt aacctggtgg tcggaaaaca tgacttcgct
      481 gacgtggaaa ccaaattcaa agagatggga tttgatcgcg tctttgcccc aagtcatgac
      541 ttagaagatg tttgtcagct gatggcccac gatatcaacc aacgtcatga cgttgatact
      601 cgtattcttg aagaggcaat ctgatgcaaa tcgtctctgt cgatattgga tcgacgtgga
      661 ccaaagcagc cctcttcgcc cgggaggggg acgcgttaac gttggttaac cacgtactga
      721 ccccaaccac cacccatcat ctggcgaagg ggtttttctc cagcctggac caggtgttaa
      781 acgtagatga tgccctcccc ctgttaaaca gtggtgaggt tgcattgaag tattcctcct
      841 ctgccaaagg tgggctggcg gttgccgcaa tgggattggt gccttctatc accctggaaa
      901 ccgcgaaagt aaccgctcac tctgcgggtg ctaaaattgc gcaatcttac gcctacaagc
      961 tgaaccgccg tgatatccag gcactggaag aaacgcagcc cgatattctc ttgttcaccg
     1021 gcggcaccga tggcggagaa gaaagctacg gactgaataa cgcccgtgtg ttggcagaat
     1081 ccaaactcga ctgcgccatt atttatgccg gaaaccggga tattcaggac gaggtgcagg
     1141 agatcctcgg gcataaagac ctgaccatcg tcgacaacgt tctgcccgat cttgaccatc
     1201 cgaacccttt agctgcacgc caggctatct gcgacatatt ccttaaacgc atcgttaaag
     1261 ggaaagggct ggatgtcgtt gttgataaaa caggcgaaga accaatgccg acaccgtgga
     1321 cagtctttga actggttaag gcaatcagta atgtcgatca cagctggaaa gagttcatgc
     1381 tgatcgacat gggtggcgca accaccgatg tctattccgc cagtgccaac acgctctcgc
     1441 ccgacactgt tttacatggc gtaccggaac ccttcgtaaa acggacggtg gaaggagatc
     1501 tgggaatgcg cgtctcggca atggtggttg gtgaaagtgc gaaagagtta gtcaatgtga
     1561 attttgctca gcaacccgca cagctggacg ctttttatca ttacctccgt catctagtcg
     1621 ctcatcccga ctacttaccc gcgaatgcag aagaaaaata tttcgatacc gtcctggccg
     1681 gactgtgtgt gggttatgcc accgaaagac atgcgggcac caaaaaagag gtctgcacct
     1741 gcgtcggcaa tgtcgattta cagatgggac gtgacctgac aaccgtgcgt aaagtcgtcg
     1801 gatccggcgg atggctctcg cgtgccagtc agttcgatat gcatcactgg ttgaaatacc
     1861 gcgagttaaa cgacgacggt aaacgcattc tcttaccgac cgaatttgaa tattaccgcg
     1921 attcccgggg gctattacca ctgctggcaa acgtcgccag agtcgatccg ctggccgccg
     1981 cgcgcaccag tattcaatgc ttaaccctat aacaattaag gctgcaatca atggaacttc
     2041 gaaataagaa attaacccat gacgaattca tgaccgagcg gcaacaggta ctgaaaacct
     2101 gggaaaccgg caaggatgtt gaaaactttg aagatggcgt taaataccaa caaaccattc
     2161 ctgagcataa acgcttctct cttgcgctgt tgaaagcaga caaggaaggt aaaaccctta
     2221 gccaaccgcg cgcaggtgtg gcgctgatgg acgaacacat cgaactactg aaaacactac
     2281 aagaagagtg cgatctgctg ccgagcacca tcgatgcata tacccgtctg aatcggtacg
     2341 aagaagcggc tgtagggatt aaaaaatcca tcgaagccgg aacctctaaa ctgaacggtt
     2401 taccggtcgt taaccacggc gttgcagctt gccgtcgcct caccgaaact ctgcaaaaac
     2461 cgctacagat ccgccacggc acgccagatg cgcgtctgct ggctgaaatc tcgatgtgta
     2521 tacatgaata tactctaaat aagtcaagtt gcaggaaggg aacaaataat gaatttccag
     2581 aagcttacat aagtaagtga cctgggtgag cgaacatcga tgcagcacat gcaacttgaa
     2641 ttatgacaag tat
//
LOCUS       DAZGAP010000348         2647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGAP010000348 DAZGAP010000000
VERSION     DAZGAP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.350"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..319)
                     /gene="ymgF"
                     /locus_tag="ACHLAM_005194"
     CDS             complement(101..319)
                     /gene="ymgF"
                     /locus_tag="ACHLAM_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO7856554.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQH"
     gene            complement(451..1974)
                     /locus_tag="ACHLAM_005195"
     CDS             complement(451..1974)
                     /locus_tag="ACHLAM_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7856555.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIREALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2310..2558)
                     /gene="ymgC"
                     /locus_tag="ACHLAM_005196"
     CDS             complement(2310..2558)
                     /gene="ymgC"
                     /locus_tag="ACHLAM_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO7856556.1"
                     /translation="MNNSIPERFILQCALFKNLEREVFMTHGYVDSYIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
BASE COUNT          809 a          583 c          544 g          711 t
ORIGIN      
        1 agttgacaac tatttgcgca ataattcctt tcttcaatga tccaggcgtt cactgccagc
       61 aacccattca gccgcatact gacagtgaga aactgaaaga ttagtgctga tccctgtggc
      121 ttacgagtct ttcccttaat cgattaatca gattgatcgc cagccaaaca agggcattaa
      181 gcaaatttat cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga
      241 ccaggagagt gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca
      301 gattattact attgttcatg ctactaatgg caatcctgtc aaaactttca aacaattata
      361 gcaaacaaaa taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt
      421 acaccgcact atggccttac tgatcgattt tcactcaacc acaaccttca ccttcggttt
      481 agaaaggata attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg
      541 ctgaaatgtg atattatttt gattcagata gtcaagttgt tctttcgttt cgacaccttc
      601 agcgacgata ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt
      661 tgattctggg tctgcgttga cgcggccaac gaaactgtga tcaattttga tataatcaat
      721 atgcaggtca tgcagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat
      781 gacaaaacca ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacatttaa
      841 tggctcacgc tcggtaactt ccagaacgag gtttaaatcg cggcgggtaa aactgtcacg
      901 gtaatttaag cactcgtcga caaacgtcgg cgaaataata tgcgaggcgc taaaattaat
      961 tccaatatga aaaccttccg gcaatttact cgcgatagcg ttcatctgtc tggcaacctg
     1021 attcatcagg cttcgcgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa
     1081 tgccgcaggt gatatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc
     1141 ccgcaatgtc ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt
     1201 atttatagcc cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat acctatcaag
     1261 caggaaggca gcggcgcagg cgattaacag aataaatatc aaaatcccaa agcctttatc
     1321 gataagtctg tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt
     1381 tgtagagtga accttctcgg tatagggata gtgtccatta agcgtcatca catcacccgt
     1441 aggtccaata atgtcatctg ccacgcgtaa tacatacctt acccctttca agggtacatt
     1501 aagcgcctcg cgaatatgct gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg
     1561 gctctgatag agcaggatag gtaatctatt aacggtgtcg attgccggag ccagcagtaa
     1621 attactgtcc gggaaaacag gaatacgaga cagtaggacc cgattccccg gtaaggatgt
     1681 acaccagacc gttccctgtt tgagaatgat gattgtgcgc aggtgaggct tcagagctgc
     1741 ttcagtgcca agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc
     1801 cgtttgagtc gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct
     1861 cgctcccgcc aggtactctg ctttcgcgga ataccagagc tggatgttca aaatagcgac
     1921 accggttata aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcattcgctt
     1981 gtcccggaca aagggtttaa tacatgaaaa tgacgttcat tcgctcgcgc tggcgtctac
     2041 agagagcatg gtagagagca tcacaaatat ccacgccgaa gataaaattt tctccaactt
     2101 catctaacct attaattatt gagcacaatg taggcagaat taaacaaatt aggtgggata
     2161 tcaggcgtca agaatgaaaa acgagcgctc tccattcctg tttgccccct gatattgcgg
     2221 atatattaag aaagcataac gcctgaaatg ctcactttgc accagcatgg tgatacagct
     2281 gatgtttatt ctataacctt actcaagttc taagagatca cggattccct gtcattaata
     2341 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
     2401 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
     2461 taactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
     2521 cattgcaaaa taaatctctc tgggattgaa ttattcatgt cagcctcgta gaaatattaa
     2581 cccaataaaa ttatgctgtg tatagtctaa agcgacagac aaaaataaga tgaatgagtt
     2641 ttctata
//
LOCUS       DAZGAP010000349         2623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGAP010000349 DAZGAP010000000
VERSION     DAZGAP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.351"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..297
                     /locus_tag="ACHLAM_005197"
     CDS             <1..297
                     /locus_tag="ACHLAM_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7856557.1"
                     /translation="YSTKYIFELARQGYKEAEFLTERSASTIAELIVSLKLLLDCQVV
                     VVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSDSGLIGATLWDRDCIT"
     gene            346..1818
                     /locus_tag="ACHLAM_005198"
     CDS             346..1818
                     /locus_tag="ACHLAM_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7856558.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            1822..>2623
                     /locus_tag="ACHLAM_005199"
     CDS             1822..>2623
                     /locus_tag="ACHLAM_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7856559.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLL"
BASE COUNT          655 a          430 c          650 g          888 t
ORIGIN      
        1 tatagcacca aatatatttt tgaattagcg agacagggat ataaggaagc agaatttttg
       61 acagagcgtt ctgccagtac tattgctgaa ttaatagtaa gtttaaaatt actgcttgat
      121 tgtcaggttg ttgttgtcgg aggaagtgtt ggacttgctg atgggtatat tcagaaagtg
      181 tcgaaacatc tttccatata ttctgaaatt tgtaatgtaa tgctgtttcc ggcatatttc
      241 agaagtgact ctggtcttat tggtgcaact ctttgggacc gggattgtat tacttaaagt
      301 aatactccag tgtattataa atccgctatt attgtgaggt ttaatatgga ctcctgtatt
      361 cagaaaaagc aggtttggta taagcatctt tcacccagac agtggaagat attcggtgcg
      421 gcatggacag gttatttact tgatggtttt gatttcgttc tgatatctct ggttcttaca
      481 gaggttcagc atgaattcgg cttgacgaca atagaagcgg caagccttat ttccgctgct
      541 tttatttccc gatggtttgg ggggcttgct attggcgctt tatcggacaa gatggggcgc
      601 aggatggcaa tggttttaag tatagtgcta ttctctctcg gaacccttgc ctgtggtctg
      661 gctcctggat atgcagtgat gtttattgct cgcattgtta ttgggcttgg tatggcaggt
      721 gaatatggct caagtgtaac atatgttatc gaaagctggc ctgttcatct cagaaacaag
      781 gcaagtggtt ttttaatttc tggattttct attgggggag gtttagctgc acaggtttat
      841 agcattgtag taccactatg gggctggcgt tctcttttct ttgtcggcat gcttccaata
      901 ttatttgctt tttatcttcg caagaatctt ccggagtctg atgactggca gaaaaggcaa
      961 caagagaata aacccgtcag aacaatggtg gatattctgt atagagaaaa gaataaatac
     1021 ataaatattc ttctgtcttg tattgcattt gcatgtctat atgtttgttt ttcaggcgtc
     1081 actgcaaacg ctgctttaat cacagttatg gcattgtgct gtgcggctgt ttttatcagt
     1141 tttatatatc agggtatggg gaaaagatgg ccaacaggca ttatgttgat gcttgtggtt
     1201 atgttctgtt tcctctacgg atggccactg caggcttttc ttcctacatg gcttaaggtt
     1261 gatatgcagt attcaccaga aactgttgct ctcatattta tgctggcagg ttttggctct
     1321 gcagctggaa gttgtatagg tggttttatg ggggactggc ttggaacccg aaaagcatat
     1381 gttatatcac ttcttatcgg tcagcttgtt attatccccg tttttcttgt ggaccgggac
     1441 tatgtatggt tacttggcct tctgatcttt actcagcagg tttttggtca ggggattggt
     1501 gctctggtgc cgaaaataat cagtggttat tttaatgtgg agcagcgtgc ggcaggactg
     1561 gggtttattt ataatgtagg ttcacttggt ggagcatgtg cgcctattct tggtgcagtt
     1621 gttgcgtctc atacaagtct tggtacagca atgtgttctc ttgcttttat cttaacattt
     1681 gttgttcttg tccttattgg ttttgatatg ccatcacgtg tacagagatg gatccatcca
     1741 gaagctgcgc ttgaatatga tactgtcgat ggaaaacctt tttatggagc aagaaaaaag
     1801 aatgttgcag gagaataacg gatgctatca gggaataaaa aaatgattca ttacagggag
     1861 aatatatatt ctcttgataa ttatgagcgt tacataactg acagttcgct atttgagaat
     1921 actgacgatg gcataatata tacaggtatt gtgcccctta ttacagtggt tgagcctgtt
     1981 gtatctaatc aacatagtgt gctgattata cctggaggtg gatacaggag gattgtttat
     2041 gaaagagagg gacttgatgt aggcagatgg atagcaggtc agggatatac ggcatttatt
     2101 ctttcttaca gaatgccctc cgttaatggt tattattcct ttgcggatgc gaagaatgcc
     2161 ctgaacttta tttcttactg ggccggaatg tcggagagaa catttaagag tgttggatgt
     2221 atggggtttt cggcaggtgg tcatcttgct ggatatatta ctcatctcca ttatgaggaa
     2281 gagacttctg tgcgtcctga ctggatggct ctgatatatc cagttatatc tcttgacagg
     2341 agtattgctc atcagggatg ccggaaacgt cttctggatg caggtgaaag tgaatattca
     2401 gccagtcttg aaaatagtgt tacagagtat atgccaccag tctttattgt gcatgcaaca
     2461 gatgataagg atgttccggt tattcactct ctgagatatt ttaactctat ccgggataag
     2521 ggacatcatg cagaagctca tttttttgat aagggaggtc atggatttgg tgtatccgga
     2581 aaaaatggat atccgataga aaaatggcaa agcctgcttc tgg
//
LOCUS       DAZGAP010000350         2620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGAP010000350 DAZGAP010000000
VERSION     DAZGAP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.352"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..196
                     /locus_tag="ACHLAM_005200"
     CDS             <1..196
                     /locus_tag="ACHLAM_005200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856560.1"
                     /translation="LLAKRFSLDFFAHSSVFLILNLQYHWGLTSLWGNWWPLTDCFMR
                     KSDITFYSLHSRKCDIARTM"
     gene            453..596
                     /locus_tag="ACHLAM_005201"
     CDS             453..596
                     /locus_tag="ACHLAM_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO7856561.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            609..818
                     /gene="cspE"
                     /locus_tag="ACHLAM_005202"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             609..818
                     /gene="cspE"
                     /locus_tag="ACHLAM_005202"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7856562.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            984..1793
                     /gene="rlmA"
                     /locus_tag="ACHLAM_005203"
     CDS             984..1793
                     /gene="rlmA"
                     /locus_tag="ACHLAM_005203"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO7856563.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1790..2356)
                     /gene="mntP"
                     /locus_tag="ACHLAM_005204"
     CDS             complement(1790..2356)
                     /gene="mntP"
                     /locus_tag="ACHLAM_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO7856564.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(2488..2578)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          689 a          610 c          632 g          689 t
ORIGIN      
        1 tttattagcg aaacgtttct ctcttgattt ttttgcgcat tcatcagttt ttcttatttt
       61 aaatttacaa taccattggg gattgacttc tctttggggt aattggtggc cgttaactga
      121 ctgttttatg agaaaaagtg atataacttt ttattcattg catagcagaa aatgtgatat
      181 tgcacgcact atgtaataac ttctcccact ggcctggaac aactgaactt attgaactat
      241 gttagaaaat acgtcagttt aagtatctgc ctgaactggc aaggttaagc acaatgatat
      301 atcggcgcgt attccgttgc ataagtgtgc aaaaaaagtg gaagacgtat cgagatttgt
      361 gcgtctgatc gagacatgtt taaaaatggc ttgccataat taacgttgta tgtgataaca
      421 gatttcgggt taaacgaggt acagttctgt ttatgtgtgg cattttcagt aaagaagtcc
      481 tgagtaaaca cgttgacgtt gaataccgct tctctgccga gccttatatt ggtgcctcat
      541 gcagtaatgt gtcagtttta tctatgttat gcctgcgggc gaagaaaaca atctaaggaa
      601 tttttcaaat ggcaaagatt aaaggtcagg ttaagtggtt caacgagtct aaaggttttg
      661 gcttcattac tccggctgat ggcagcaaag acgtgttcgt acacttctct gctatccagg
      721 gtaatggttt caaaactctg gctgaaggtc agaacgttga gttcgaaatt caggacggtc
      781 agaaaggtcc ggcagctgtt aacgtaacag ctatctgatc gaatccactg atctgaagtg
      841 tgaatacgct tcaatctcgc tataaagcct cgtcgaatgc gaggcttttt actttgcttt
      901 atcttcgctc ctggcgttcg gatatttgcc cgccgcgtga ttcgcgttac acttgcggcc
      961 tttagtatcc tgccggagtt gtcatgtcgt tttcctgtcc actttgccat cagcctcttt
     1021 cgcgtgaaaa aaacagctat atctgtcccc agcgacatca gtttgatatg gcgaaagaag
     1081 ggtatgtcaa tctgctgccc gttcagcata aacggtctcg tgatccgggc gacagcgcgg
     1141 aaatgatgca agcacgccgc gcattcttag atgccggaca ttatcagccg ctgcgtgatg
     1201 caattgtcgc ccaactgcgg gaacggcttg atgaaaaggc cacagcggtg ctggatattg
     1261 gctgtggtga agggtattac acacacgcat ttgccgatgc gctgcccgaa atcaccacgt
     1321 ttggcctgga tgtctcgaag gtagcgataa aagcggcggc gaaacgctat ccgcaggtca
     1381 ctttttgtgt tgcttccagc caccgtttgc cgttttccga tactagtatg gacgccataa
     1441 tacgtattta cgcgccgtgt aaagcagaag aattagcgcg agtagtgaag cctggcggct
     1501 gggtcattac tgccacgccg ggaccgcgac atttgatgga gctgaagggg ctgatttaca
     1561 atgaagtaca tcttcatgca ccacatgcag aacaactgga aggttttaca ttacagcaga
     1621 gtgatgagtt gtgttatccg atgcgtcttc gcggtgatga agccgtcgca ttattgcaga
     1681 tgacgccgtt tgcctggcgt gcgaagccag aagtctggca aacactggca gcaaaagaag
     1741 tgttcgactg ccagacggac tttaatattc acctctggca gcgttcttat taaccgtgga
     1801 agtgcgtcca gaggatctgg acgccgatgc cgatcagcac cagcccgccg agaatttccg
     1861 cttttttccc aataattgag ccgataaagc gaccaaccat catccctaat gtcgacataa
     1921 tcaaggttgc acaaccaatg gccaatgcgg tcgcgataat gttgacctgc aggaaggcga
     1981 gaccaacacc cacagccatg gcatccaggc tggtggcaat cgcggtggtt accagtagcc
     2041 agaaaccgtg ccgacggcgc ggctcttcat cttcatcatc tgcgccacga aaaccctcaa
     2101 taatcattcg cccgccgagg aatatcagca gcacaaacgc aatccagtga ttccattcaa
     2161 ggacaaaccg gctggctaac atgcccattc cccagccgat cagcggtgtc agggtttcga
     2221 cggcaccaaa aataaggccg gttcgcaatg cttcagaaaa tttcggttta tggagggtgg
     2281 cacctttacc gattgatgca gcaaatgcat ccatcgacat accaaacgca agaagaacag
     2341 tagcagtgat attcatgaca acgtcctgac cggggtatcc ataaaacaca tcatcgcccc
     2401 cagcaaacat gcatacatga acaacaagcc gcgatgggtt ttcgcgttca tgcaggcata
     2461 gcgattgatg tgcatatggt ctcgcctgac tgaaagtgtt cagtccgtac gtgccacgtt
     2521 ttgcaacgag tatgttgaca cgtacatttc cggaatctgg aaatcggcta ctccccaaat
     2581 gagggcgcaa ccttaacata ttttgagaat ataaaacaac
//
LOCUS       DAZGAP010000351         2564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGAP010000351 DAZGAP010000000
VERSION     DAZGAP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.353"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..542
                     /locus_tag="ACHLAM_005205"
     CDS             <1..542
                     /locus_tag="ACHLAM_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO7856565.1"
                     /translation="KIELHRSSPFYAEPVDCVIWIKNNLIEANDYNPNVMAPSEKRLL
                     THSLLVDGFTQPVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPVTCIKHDNN
                     FKAYSIASTIRHNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTDEVLRLKQI
                     SGLTELFEEENFSPAWTIK"
     gene            complement(724..2469)
                     /locus_tag="ACHLAM_005206"
     CDS             complement(724..2469)
                     /locus_tag="ACHLAM_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7856566.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT          776 a          633 c          516 g          639 t
ORIGIN      
        1 taaagattga gttacatagg agtagtcctt tttacgcaga acctgttgat tgtgttatat
       61 ggataaaaaa caatctaatt gaagcaaacg actataatcc taacgtcatg gctccctccg
      121 aaaaaaggtt attaacacac tccctgttgg ttgatggatt cactcaacct gttgtcacct
      181 tagctcaaaa ggggaaatac accgttattg acggatttca cagacaaatg cttgggaaaa
      241 gcacaacaaa actagagaaa aaactaaaag gatatttacc tgtcacatgc attaagcatg
      301 ataataactt taaagcttac tctatcgcct cgaccatcag gcataacagg gccagaggaa
      361 agcaccagat agacgcgctc tccgatatcg tccgagatct ttcccgaatg ggatgggatg
      421 atctccgaat aggtagtgaa ctgggcatgg atacagatga ggtactgaga ttaaaacaaa
      481 taagtgggtt aacagaacta tttgaggagg aaaactttag tccggcatgg acaataaagt
      541 aatcatttag ggggtgactt tatccagtaa ttaccgactg atatatgatc accccatgta
      601 atggacagct attgacagat caaaaacatg ggggatatta attcaactct gaatactcgc
      661 taaataccca tcttagatta tcaaaaaacg aatgattaca tatcagcaat aataaaacac
      721 aacttataca aaatcctcaa gatgacgact gatactgttt tcacgataac aggtcaccag
      781 taattcccgt gtagcccggg tgaacgcaac ataaagcgcc ggaataactt cagactcatc
      841 cgcagcacct gagtgaacga aagaagcatt gataaccgca acatacggaa actccagtcc
      901 cttactgctc tggtaggtca gtaaatgcac aacatccttg cgtcgcgaat atttcttctt
      961 accttcactg tcaaagcagg tggcataagg tatcccgcgt tgtgtcatga cctctttcag
     1021 atgtttcact gaaaactctg caggacacaa aacagccata tcactccagt gaccgcacag
     1081 tgtatagcgt ttctccagcc agcccacaac ctgcactgcc tcatcactct cagaagcaca
     1141 acgcagaatt tccggctctg tgccctcttc accgcccgcc tgtggctgaa caaacggtat
     1201 ttcacggttc tgatgcttct caaaatactc acgggagaat gcataagcaa agtgcagaat
     1261 gcgtttcgta ttacggtaat ttaccggcag gacggaagta cgtccctgcg cctgaatccc
     1321 cacactggca agcgaaaagt tcagagcccg ttcccgtctg taaatggact gtgcgtcatc
     1381 gtacatcagg agcagtgaac gggacgcgtt atcaaacagg cgggctatca gagccagcca
     1441 gcgactctca aaatcatgcc cctcatccac aagtacggca tcgtatccag tatcagtaat
     1501 tgttccgctg ttcacggcat cttccagcgc actgaagcat ttctccgggt aatcctttcc
     1561 tccagcagtc acctgaattc catggcgctt tatcattgag gcgcaccaac tatgaaaatg
     1621 gaataccgta acccgtgact tcagtcccct ggactcaata cattcccgca ggtagctggc
     1681 cagagtaatg ttgtaacata gcaccagaat cttaccaggg gtagtttcag ccaagtaaag
     1741 acaacggaac aaaagaatca ttgtttttcc agagccggct accccatgaa taacacgatg
     1801 cccatcacca atatttctgg ccagaatctc ctgctgaata tccattattt tgatttgtga
     1861 gtgctgtctg acggtcattt caggaaacag atgagcacgc agaatatctc taacctgtgg
     1921 tgtcacccgg gttctgaatc ccatcgtaaa catcccggca attttttggc ggaatatatc
     1981 aggtaaaaca gactgagtca tatcatcctg acagattgtc tgcgctgaag ggaaaatcct
     2041 ttccacagca ttttcatcat tcccagccag agccttcagt tgctggcggg taatacgggt
     2101 aaacacaaca ccatacgccc aggccagatt cagacgcccc ttatactgac catcattctg
     2161 tcgcaaacgt gagtctgcag gtaaagcatt tacggtatca caggcataac gacgaacctg
     2221 tagcagcgga tttatttctg atttaagaat accgttagtt tcaagaatga cctgttcgtg
     2281 atttacctga tgcaacgtgt gaacggtcca gtccttgact tcaagaaata ccagaccatt
     2341 ctccgggtca ataatgacaa aatcaggatg tcggtttttc cgtcccaccg gaatatcgta
     2401 ccagaccaga cactcattac tcataaatgc ctcaagacgg gatgcaaccc gcttttcccc
     2461 ggcagtcatc cgctcctcgg taacacctga tatatttatc agtttagcca taaaacagcc
     2521 cctgacagat gatgaacatt taatatatat aacttatcat atat
//
LOCUS       DAZGAP010000352         2507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGAP010000352 DAZGAP010000000
VERSION     DAZGAP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.354"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            190..384
                     /locus_tag="ACHLAM_005207"
     CDS             190..384
                     /locus_tag="ACHLAM_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7856567.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            745..1038
                     /gene="iss"
                     /locus_tag="ACHLAM_005208"
     CDS             745..1038
                     /gene="iss"
                     /locus_tag="ACHLAM_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO7856568.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(1129..1311)
                     /gene="rzoD"
                     /locus_tag="ACHLAM_005209"
     CDS             complement(1129..1311)
                     /gene="rzoD"
                     /locus_tag="ACHLAM_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO7856569.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1528..2025)
                     /gene="rrrD"
                     /locus_tag="ACHLAM_005210"
     CDS             complement(1528..2025)
                     /gene="rrrD"
                     /locus_tag="ACHLAM_005210"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO7856570.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(2025..2240)
                     /gene="essD"
                     /locus_tag="ACHLAM_005211"
     CDS             complement(2025..2240)
                     /gene="essD"
                     /locus_tag="ACHLAM_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO7856571.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          704 a          606 c          547 g          650 t
ORIGIN      
        1 ggatcgccgg aaaggacccg caaaaatgat aataattatc atctacatgt cacaacgtgc
       61 atctacgcca tcaaaccacg tcaaataatc aattatgacg caggtatcgt attaattgat
      121 ctgcatcaac ttaacgtaaa aacaacttca gacaatacaa atcagcgaca ctgaatacgg
      181 ggcaacctca tgtcaacgaa gaacagaacc cgcagaacaa caacccgcaa catccgcttt
      241 cctaaccaaa tgattgaaca aattaacatc gctcttgagc aaaaagggtc tgggaatttc
      301 tcagcctggg tcattgaagc ctgccgtcgg agactaacgt cagaaaagag agcatataca
      361 tcaatccaaa gtgatgatgg atgaacatcc cggtttcttc caccatcgca ccggaaaagc
      421 gactatgagg gtaaccctgc gtctgtcagc acagtaaaac ccggtgtgca tcgtttttga
      481 ttattcccgc acactcacgc agaaggaatt ccccgtcggg ctacggtcat ggttaatgcg
      541 ggaatacggc gacgatacag cgcagctaaa agggtaatgg acagatagag cggtttattt
      601 cattccacag gattctgagt gccccccctc ctccaatagg ctgagcatcc acctatatag
      661 ttttaatttt catcaatcca tttaactatc gtttaattgt tgtcacatag gattctgccg
      721 tttttaacaa tgcaggataa taagatgaaa aaaatgttgt tttctgccgc tctggcaatg
      781 cttattacag gatgtgctca acagacgttt actgttggaa acaaaccgac agcagtaaca
      841 ccaaaggaaa ccatcaccca tcatttcttc gtttcgggaa ttggacagga gaaaactgtt
      901 gatgcagcca aaatttgtgg cggcgcagaa aatgttgtta aaacagaaac ccagcaaaca
      961 ttcgtaaatg gatttctcgg ttttattact ttaggcattt atactccgct ggaagcgcgt
     1021 gtgtattgct cacaataatt gcatgagttg cccatcgata tgggcagctc tatctgcact
     1081 gctcattaat atatttctgg gttccttcca gttgtttttg catagtgatc agcctctctc
     1141 tgagggtgaa ataatcccgt tcagcggtgt ctgccagtcg gggggaggct gcattatcca
     1201 cgccggaggc ggtggtggct tcacgcactg actgacagac tgctttgatg tgcaaccgac
     1261 gacgaccagc ggcaacatca tcacgcagag catcattttc agctttcgca tcagctaact
     1321 ccttcgtgta ttttgcatcg agcgcagcaa catcacgctg acgcatctgc atgtcagtaa
     1381 ttgccgcgtt cgccagcttc agttctctgg catttttgtc gcgctgggct ttgtaggtaa
     1441 tggcgttatc acggtaatga ttaacagccc atgacaggca gacgatgatg cagataacca
     1501 gagcggagat aatcgcggtt actctgttca ttgctgaccc cacaaacaga tttcacgctc
     1561 aatctcacga cgagtcatga gacctttcca ttgcttaccg ccagcatatg tccagcgacg
     1621 tagctgatca catgcgcctt tgatatcgcc ctggtttatt ttgcgaagaa gcgtcgatgt
     1681 tctgaaattg ccagcaccca cgttgtaaac gaatgagtaa agagcgccgc gcattgtttc
     1741 cggtatatcg actttgatgt acgggttaat ttgtctggcg accgtggcaa ggtctttatt
     1801 caggagggct ttgcattctg ctttggtata cgttttaccg agcatgatgt cttttcctgt
     1861 atgcccgtga catacagtcc atacaccaac aatatctttg tatggtatgt agctgacacc
     1921 ttccagacca tcgttaccac ttgggccagt gattaacact gatgctatag caattgctcc
     1981 gccaccaata gcagcagcaa cggcttttcg taatgatgga ggcattattc acctctcgca
     2041 gccttgcgct tatcttcttt aatcttgaaa taaaggtttg tcaggtacgt cagcaggcca
     2101 aataccaggc tacccagcac acctattgct gcccactgtg agggcgtgac tttatcgagc
     2161 agctgtaaaa accagtaacc ggcactacct gctgaggtgc cataggcgac acccgttgtt
     2221 aacttatcca tggatttcat aaccccacct cgcagacaaa gcgggtgtaa attgagggaa
     2281 tacaacgtat cgcaaaaaag cagaaacgta acagactcgg agtcagtgaa taactcaggt
     2341 attgggttat cagctaatat cgagactcaa aaaatggaaa aacccgctcg acggcgggtt
     2401 taagctgtgt gacgaagtaa ccactcttaa cagcataacc aattttttac gtacgtaaac
     2461 cacaaaatga tatttgcgag aatgctaccg agtattgaaa acaccac
//
LOCUS       DAZGAP010000353         2489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGAP010000353 DAZGAP010000000
VERSION     DAZGAP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.355"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..169
                     /gene="hemP"
                     /locus_tag="ACHLAM_005212"
     CDS             <1..169
                     /gene="hemP"
                     /locus_tag="ACHLAM_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO7856572.1"
                     /translation="LTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDHDGQEYLLR
                     KTQAGKLLLTK"
     gene            complement(173..1609)
                     /gene="selO"
                     /locus_tag="ACHLAM_005213"
     CDS             complement(173..1609)
                     /gene="selO"
                     /locus_tag="ACHLAM_005213"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO7856573.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYASRPPDWGKRLEVSCSS"
     gene            complement(1672..2385)
                     /gene="ydiV"
                     /locus_tag="ACHLAM_005214"
     CDS             complement(1672..2385)
                     /gene="ydiV"
                     /locus_tag="ACHLAM_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO7856574.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
BASE COUNT          675 a          647 c          574 g          593 t
ORIGIN      
        1 actcacgcct gaaacggatg ccaatcacaa gaccgcttcc ccgcagccca tccggcgaat
       61 taccagccag acactgttag gtccggatgg caaactgatt atcgatcatg acgggcaaga
      121 atatctgctc cgtaaaactc aggctggcaa gctgctgttg accaagtaac ctttaactcg
      181 agcagctgac ttccagccgt ttaccccagt caggtggacg actggcgtag tcatcatctc
      241 tgtcgctgaa aggatttcgc aacgcctcat gcaggcggtg caattccgtc atatcaccct
      301 tttctgccgc ttcaatcgcc cgttgcgcca accagttgcg caacaccaga gctgggttaa
      361 cgctttgcat cagttgctga cgctcaatat cgctaacctc gtcttgttgc aaacgcctcc
      421 gataacgggc aaaccagtca tcaaatgccg cacgatcaat aaactcatca cgtagcggtg
      481 acgccgcgct gtgctgctcg gtcagactca gcatgcgaaa tgtgcgggta taatcgctgc
      541 gctctcgcgc catcagactg aataattcat ttagtagcgc gttatcctct ttttgctccg
      601 tcatgaagcc cagtttctgc cgcatccgtt gtccataatg cgtcaacaaa acctgctgat
      661 agctgtccag ggcctcattc agggcatcaa cggcgacaaa tggcgacaat gtctgcgcca
      721 gacgctgtaa attccacaac gcgacggcag gttgattatc aaagctgtaa cgcccttggt
      781 gatccgagtg attacaaata aaaccgggtt cgtaatcatc aagaaaacca aacggcccgt
      841 aatcaagcgt cagccccagc agcgacatgt tgtcggtatt catcacccca tgagcaaagc
      901 cgaccgtctg ccattgggca attaacgatg cggtacgtgc gacaacatcg ctaaaccaga
      961 gacggtattt gtcctcatca tcttcaagat gagaccagta atgacgaatg gcaaagtcag
     1021 ccaactgacg aaccttttcc ggctcgcggc gatagtaaaa atgttcgaaa tgaccaaagc
     1081 gcagatgact tagtgccaca cgcatcagca tcgcgcctgg ctccaccgtt tcccgataca
     1141 ctggcgaatc gctggtgacg atacttaacg cgcgggtcgt cggaatgccc agataatgca
     1201 tcgcctcact ggcgagactt tctcgtatcg tcgaacgtaa aaccgcccgt ccatcaccca
     1261 ttcgcgaata aggcgtcagg ccagcacctt tcagatgcca gtccattgta gtgccatcag
     1321 caagccgttg ttcgccgagt aaaatgccgc gcccatcacc cagttggccc gcccagacgc
     1381 cgaactgatg accactgtaa acctgcgcca gtggtgacat accaggcagt agggtttcgc
     1441 cgccccagac acctgcgcca tttttaaaca gcgacgatgg aatactcagc gtgttagcga
     1501 gttcggtatt atgccaaatc agccgggcat tatttaaagg cgtaggggaa agtgctgtat
     1561 aggtttctgg caattcatcg cgccagcggg taacaaaaga cagggtcata gatcctccta
     1621 tttgatagtg tagacggtta ctctcgtctt aaacaccagc aaacaggagg gttatcgctg
     1681 aaccaacgtc gttatctgat ttacaggaac ggcaggccac aggcatccct ggagagcatg
     1741 gaaatcaaag ggggtaatct gtgccattat ttctggcgta tcaatgcccc ccgcgatgat
     1801 gcaattgcaa catggggaaa tttgagcctg gatcgcacga ataaacggtt cgaaggagcg
     1861 atgcgtgatt tgttgctgaa taaaactttt atccagcatg acgcgggtga aaagaccatc
     1921 aaaaaccgct ttcattgtgc tattgcctgc gcccaaatta cctaacacaa ggggatacat
     1981 ttgcgataag gacaaaagat cgcgattatc ttttccttca tcgagatgcg ggtaattctc
     2041 attaatcaac agttcaataa acggatattt taatagttcg cctgcgtaat tatccctgtc
     2101 taataataat gtagccactt gtggcgttaa attaagccag gcaaagagtt tatgttgaat
     2161 aaaaaagtgt tggcatgatt ttagtaattc caactgctct gaaaataatt gccaatgttg
     2221 ctcctcggta agttgcgcta taacccgact cgtcgggata cgaaccgtac catcttcact
     2281 ggagaaatgg gtaattaact caacgccgac aaggtcttgc tggttatctc ttatcggcag
     2341 gaaatagcaa tcagaatgat aaagattctc caaaaaaatc ttcatggtaa ccgtccctct
     2401 cgaaggatgt ttttcagtat ccggctaaaa gggatgaggc cataagacat aacaattaca
     2461 gaaggagtaa ctttcatttg ttccatgtt
//
LOCUS       DAZGAP010000354         2479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGAP010000354 DAZGAP010000000
VERSION     DAZGAP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.356"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1699)
                     /locus_tag="ACHLAM_005215"
     CDS             complement(<1..1699)
                     /locus_tag="ACHLAM_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856575.1"
                     /translation="MMNIRYPVRKADGRDYKSYDELLTDIRKNAHGWWLLGVNRYWHG
                     GIHVGRSSSPASVLNQETPEKSVPLQFMMDGEVVAWRVNRDYAAIECYQERPLRQSGT
                     FVLVKSVYKPDEQDESSWLTLYQLYMHIAPLSEFPKRPLYRVTQKGHGVRMRKYSRHD
                     DSREIAPDVLANKHGHPRTLTQGDTLAVLQQKSFLLKQRPESFALVQRLQDGKPAGEL
                     FWVSMRPEYLEPDGQCYVCLPDWMHSALNHGVFDDVVVPPVPLKVMVKAGDAVGFLGA
                     QDLADEDNYPQIITTDYKAHIELLSLDEHVPDVVANMKGIKTGKQFIKLKLKRPLYLR
                     NGEGEESTFEQMSAITRIDAGKIIPRDATYPFTDKTGITYFQIRPHTWMHQNDVEQLS
                     QHDLAGLNFHCIEAEHTTDFTRTLDERWLIDALKSISSHFDGEKGPQFAQAKMFYDSL
                     IHNAENRRAPNPYPDKSLDQYLFGALHTNQMNIPEYARRLIVKHDSCWHSTRDDTRWS
                     SVCKSRDESPVVKMANGGFLDATHWMDKVPPFASQRSVWHFHPLEFPEILRSAEFPRT
                     "
     gene            complement(2040..2254)
                     /locus_tag="ACHLAM_005216"
                     /pseudo
     CDS             complement(2040..2254)
                     /locus_tag="ACHLAM_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2260..>2479)
                     /locus_tag="ACHLAM_005217"
     CDS             complement(2260..>2479)
                     /locus_tag="ACHLAM_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO7856576.1"
                     /translation="YPAYHRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNE
                     RRRVLLTPGHYLGNWDHRLRHLQEQNVA"
BASE COUNT          584 a          644 c          578 g          673 t
ORIGIN      
        1 gagttctggg aaactctgca cttcgtaata tttccggaaa ctcaagcgga tgaaaatgcc
       61 atacagaccg ctgactggcg aacggaggca ccttatccat ccagtgtgtt gcatccagaa
      121 atcccccgtt agccatcttc acaaccgggc tttcatccct ggacttacat accgatgacc
      181 agcgtgtatc atcgcgggta ctgtgccagc agctgtcgtg tttcactatc aggcgtcgcg
      241 catattccgg gatgttcatc tgattggtat gcagcgcacc aaacaaatac tggtccagag
      301 atttatcggg atacgggttt ggcgctcttc tgttttcagc gttgtggatc aggctgtcat
      361 aaaacatttt tgcctgcgcg aactgcggtc ctttttcacc gtcaaagtgc gagctgatgc
      421 ttttcagggc atcaatgagc cagcgttcat ccagcgtgcg ggtaaaatct gttgtgtgtt
      481 cagcttcaat acagtgaaaa tttagccctg ctaaatcgtg ctgactgagc tgctccacat
      541 cgttctggtg catccacgta tgtgggcgaa tctgaaagta tgtgatcccg gttttatccg
      601 taaacgggta cgtggcatca cgtgggataa tttttccggc atcaattcgg gttatggcgc
      661 tcatttgttc aaatgtactt tcttcaccct ctccatttcg caggtacagc gggcgtttca
      721 gcttcagctt aatgaactgt ttgcctgttt taatcccttt catgtttgcc accacatctg
      781 gcacatgctc atcgagactc agtagctcga tatgggcttt atagtccgtg gtaataattt
      841 gcggatagtt atcttcgtca gccagatcct gagcgccgag gaatccgacc gcatcccctg
      901 ctttcaccat cactttcagt ggcacaggcg gcaccaccac atcatcaaac acgccgtgat
      961 tcagcgcact gtgcatccag tcaggcaggc acacgtaaca ttggccatca ggttccagat
     1021 attccggacg catcgagacc cagaaaagtt ctccggcggg tttaccgtcc tgaagacgtt
     1081 gcaccagagc aaacgactcc ggccgctgtt tcagtaaaaa cgatttttgt tgcagtacgg
     1141 caagcgtgtc accctgcgtc agtgttctgg ggtggccgtg tttgtttgcc agtacgtccg
     1201 gcgcgatttc acggctgtca tcatggcggg aatatttacg catccgcaca ccgtggcctt
     1261 tttgtgtaac ccggtaaagc gggcgtttcg gaaattctga caatggcgcg atgtgcatgt
     1321 aaagctggta cagcgtcagc caggatgatt catcctgctc gtctggcttg tacactgatt
     1381 ttaccagcac aaaggtaccg gactggcgca gggggcgttc ctgataacat tcgatagccg
     1441 cgtaatcgcg gttaacccgc catgccacca cttcgccatc catcatgaac tggagcggca
     1501 ccgatttttc aggtgtttcc tgattcagca ccgaggccgg ggacgatgac ctcccgacat
     1561 gaatacctcc gtgccagtaa cgatttacgc caagtagcca ccaaccgtgg gcgtttttgc
     1621 ggatgtcggt tagcagctcg tcatagctct tatagtctcg tccatcggct ttccgtaccg
     1681 gataacgtat gttcatcatc ttttcttaag tgaaatcaca tatttcgatg gattctcgcg
     1741 cgtattttgg gtttggggtg tagtggaacc aaaaagacgg ttaagcggtt aaattgaatg
     1801 gtcaataaca ggtaaatcaa tctgttgaga tcaacgacgt cttttgctcc gcaaacggct
     1861 aagatgagta cagataattt ggttatcgta aaaaaggtgg ctaaaaattt caacctgcaa
     1921 tgtaaattgg ccggttgatt tttggctatt taaattgacc tcttaacggc gtttattgtt
     1981 ccattgctcc ccaaccccct atggattata agccgcacat acattacttc gttctgcata
     2041 tcaccactaa tctgcttaag atgtactaac aacatcatcg cctgttggct aaacggaact
     2101 ataactcctc tttcattttc atgccgcgat acaagtaatg cacaccttta atttcttcac
     2161 gttttgccgg aatgcgccaa atagcgttat caaaatcaaa ttctttttaa cgagcaaaac
     2221 gccagtccga agactggcgc agtgataaaa cattctgcat catgccacat tctgctcctg
     2281 aagatgacgc agacgatggt cccagtttcc caggtaatgc ccgggagtca gcagtacgcg
     2341 gcgtcgctca ttacagagcg agttaccaat caccacttct ccggccacac cacacagcga
     2401 cagttgtata tacgccatgc cggctgccag cgggtcaata tccgttgccg acacccacat
     2461 cctgtggtat gccggatac
//
LOCUS       DAZGAP010000355         2459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGAP010000355 DAZGAP010000000
VERSION     DAZGAP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.357"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..406
                     /locus_tag="ACHLAM_005218"
     CDS             197..406
                     /locus_tag="ACHLAM_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7856577.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPDPRKEPPAGNHTA"
     gene            complement(426..659)
                     /locus_tag="ACHLAM_005219"
     CDS             complement(426..659)
                     /locus_tag="ACHLAM_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856578.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(748..1371)
                     /locus_tag="ACHLAM_005220"
     CDS             complement(748..1371)
                     /locus_tag="ACHLAM_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7856579.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            complement(1460..1969)
                     /locus_tag="ACHLAM_005221"
     CDS             complement(1460..1969)
                     /locus_tag="ACHLAM_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856580.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          714 a          624 c          523 g          598 t
ORIGIN      
        1 gattcggcag catttaaggg catatcagaa aaagagaagg acatgaaaga ggagagcaat
       61 aaaagacacg gaaaataaag aagagagaga aaatattttt aaaaacagaa aattaacaac
      121 gcacatcagg aaacgtcgtc tcctgattca gtgataccac atgaaaattt agtgaacggt
      181 taccgttctg aagtctatgt cagaagatat tccattacca aaaatcaatc agcgatacag
      241 agacgatcat ggcgcgcttg tcaccgtgac cagtgttgag gaaacccggg tggtgtttat
      301 gcgggacggc tatccgcatc cctgcatgcg gccgatgtac aacttcctgg gaaaattcaa
      361 acccgacccc cggaaagaac cccctgccgg caatcacact gcctgatggc agcataatcc
      421 acttattaat tctcatcccc ggagacaagc ccagaaaacc actccgggtg ttcactgtat
      481 gccgtgttca gcgcatccca agaaacgttg atataaaagc tgtcctgcaa tttgcgggta
      541 acaatttctg ccacccggtt acggttatcc gcctgtccgg cataacctct ctctgccagc
      601 cactcattca gttcatgttc cggagacaga ttgataatat cgtaatcgga ccttgccatt
      661 cccgtttcct tagcacatgt tttcaggctc ggggataaag aatgtcgtac tgctgctgcc
      721 agagcaatat tccccctgat ggtcgaatca gagatggtag gccggtactt tgcgggttgt
      781 tcccgtcaga ttatcatgca cctccgcggt cgttgtcacc accggcattc cgctctttct
      841 cagcctcagg acatcagccg caatacgctg actaccgaac cagaacaggc cgtccagtgc
      901 agtcaccagc aaccccgcct ccagcgcatg cttcagccgt tcacggggcg ctttcacttc
      961 ccgggcaatc tgctggtatg gcgatgatgt gttttcattc ccaatcaccc ggcgaatacg
     1021 atgagacaga tgataccggt atgtatccgg tacaccggaa aggctggcct tcaggctgta
     1081 cacgcagcca aatcgtttat cattgaacac cacatttttc tggctgatgc cccattcttc
     1141 acgcagcgcg gcaatcagtt gtggtgtccg ggtaagcaac aagcgaaaag gcagttcaaa
     1201 actggtgacg taatccacat tcagcagtgc aatacgcagc cgttcttccg gcccggcctc
     1261 tgtctgccgg cactcctcca gaacatcctg ccactgctgg tgaaggcgtg gaagttcatt
     1321 cagttcagta aaactgtatt tatccgcaag atagtcaatt cgtgtacgca tactgaagag
     1381 tattccgtat aaagattcag ctggcaaaac tttatcagtc tgtaaaaact aacggaagag
     1441 tcgatatttc tcccgtccat caccggatga ttgttgcaat accccgtggc atcagagact
     1501 gaacagccgt ttttaacgcc acgtattgcc ctgatgaacc aggccgggca acccgaaaac
     1561 atctttccac ccggcattgt ccgccagcac ttatcaccgg ccaggtctgt tgcagtaaat
     1621 ccaccagttg cgcacattct tcatcagcca tgacgccgac tcgcggatgg caaatactcg
     1681 tctggctgag cagcaacaga catcgtatct gtgctccttc atgttgttcc cgcaaccagc
     1741 gtaataccac cggcgctgat gggctgaccg tgtgattatg ctccgtaata cgttcgtgta
     1801 cccgtcggtg aaaggaacta caaaatgtct gaatctgttg accattgatt ccttctgccg
     1861 aatagagcgt gaagtgaaat gccaacagat ttggccagtg gtccaccgcc tgctgaacaa
     1921 aacgccggat ttcccccggc tctgaaatta aggcttcggt tatttgcacg aaatgttcct
     1981 tttcaacccc tgttgaattt tgtcaagccg gtgcaggcac agtaaagcgc gcatcaacac
     2041 ctgaaaggta cgatacaaac acaagttaca aacaataaga acggaaggac aattaatcag
     2101 tgaggaataa taactaaatt aaccaaaaga atgttactga caatactacc aatacttaaa
     2161 tagcaatata ttaattaatg taagaacaga tgcccggtaa gacatataca caatacatcc
     2221 acaaacacac tcttttcatt aaatcaatca cctgatcaaa ccatgaaagt gtgatgtaag
     2281 tcaaaattac tgacactatc aattgataat gattatctct atcattatcg tgaaattcga
     2341 gaatgaaagg agaagtagtc attataagcc ttttataaat cgtcatcatc atagtaaagt
     2401 agaacacttt ttatatgagt tactatatga aaatatactg gacaagaaaa cgcattcct
//
LOCUS       DAZGAP010000356         2377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGAP010000356 DAZGAP010000000
VERSION     DAZGAP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.358"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..276
                     /locus_tag="ACHLAM_005222"
     CDS             <1..276
                     /locus_tag="ACHLAM_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4526/YPO1902 family pathogenicity island
                     replication protein"
                     /protein_id="HFO7856581.1"
                     /translation="ARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGL
                     DVMMLAAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            291..440
                     /locus_tag="ACHLAM_005223"
     CDS             291..440
                     /locus_tag="ACHLAM_005223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856582.1"
                     /translation="MAEINFQVLREAEQVKRENQPVEAGAWKDAVEWVLQEDCQAVNI
                     RIKGE"
     gene            443..730
                     /locus_tag="ACHLAM_005224"
     CDS             443..730
                     /locus_tag="ACHLAM_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856583.1"
                     /translation="MATLQELIDLTPEQEKAWKRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTARVYMPGIDAPGLTSWADDWHGITLKDGVEVDDD"
     gene            734..1132
                     /locus_tag="ACHLAM_005225"
     CDS             734..1132
                     /locus_tag="ACHLAM_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856584.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VASCIIEDGGMCVDGVGEYVGHSLPGGTYQLYTASPVPVVPEGLVKAVRFYEQVKREN
                     PPVETGAWKDAVEWVLKEACQAVNIRIKGE"
     gene            1135..1626
                     /locus_tag="ACHLAM_005226"
     CDS             1135..1626
                     /locus_tag="ACHLAM_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO7856585.1"
                     /translation="MQISPVTLRVAKAFISRHHRHNKPPVVHKFSIGLRNDAGELTGV
                     ATAGRPVARHFDDGLTLEVNRTCTTGERNANSALYGAVWRAAKDMGYRRCITYTRADE
                     SGASLRADGFVRVKELPSGKSWAESSVALKSTRDPVGSGGVPRVLREIRRMSTTVIHI
                     KGE"
     gene            1630..2097
                     /locus_tag="ACHLAM_005227"
     CDS             1630..2097
                     /locus_tag="ACHLAM_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856586.1"
                     /translation="MAIAERMGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            2115..>2377
                     /locus_tag="ACHLAM_005228"
     CDS             2115..>2377
                     /locus_tag="ACHLAM_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856587.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQL"
BASE COUNT          642 a          495 c          718 g          522 t
ORIGIN      
        1 gcgcgccgtc gcattgccgg tattgatgtt cgcccgggac gtggcatcac actgagtgat
       61 gaggaaagtg cagagctgtg gcgtttatgg cagaagagcg gcatcacaga tgtggaaagt
      121 gcggacggac tggatgtcat gatgctggca gctgagcaaa tgaatatccc gctgacggcg
      181 gtatggcatg cagtgagggg atggcatcag ggaactgcgg ggtttaatga tgtatcggcc
      241 agtcgccgga aactgaaccg ggaaaaagca gaatgacaag gacaaatgac atggccgaaa
      301 ttaactttca ggtactgcgg gaggctgaac aggtgaagcg agagaaccag ccggttgagg
      361 ctggagcgtg gaaagatgcc gttgagtggg tgctccagga ggattgccag gctgtaaaca
      421 ttcgcatcaa aggagagtga acatggctac tttgcaggaa ttaatcgacc tgacgccaga
      481 acaggaaaaa gcgtggaagc gccttgtgaa ggctgtaaag gatttcaggg cagccggagg
      541 aaagttttat agcgtcctgg acacgctgag tgcatacaac ggcgagcacg ttgccagcat
      601 tgataacgat aagggctacc acactgcaag agtctatatg cctggcattg atgcgccagg
      661 gctaaccagt tgggctgatg actggcacgg catcacgctg aaagatggcg ttgaagtgga
      721 tgatgactaa cacatgacca cgattaccaa agagcgactg caatggctgg ctaacatttc
      781 tggccgcgat gacattgacg atatagacgg cggtgagatt cgtgagctgg cgcttatcgc
      841 gctggcatcg ctggaagcag agcctgtggc gtcatgcatt attgaagatg ggggcatgtg
      901 tgttgacggg gtcggtgagt atgtaggtca ctcgctgcct ggtggaacgt accagctata
      961 caccgcttca cctgtgccgg tagtgcctga aggactggtt aaagcagtgc gcttttatga
     1021 acaggtaaag cgtgagaatc caccagttga aactggggca tggaaagatg ccgttgagtg
     1081 ggtgctcaaa gaggcttgcc aggctgtaaa tattcgcatc aaaggagagt gagcgtgcag
     1141 atttcaccag tcactcttcg tgttgcgaag gcgtttatat ccagacatca ccgacacaat
     1201 aaacctccgg tggtgcataa attcagtatt ggtctgagaa atgatgcggg tgaattgaca
     1261 ggtgtggcga cagccggcag acctgttgca cgccactttg acgatgggct gacactggag
     1321 gtcaatcgta cctgtactac tggtgaacgt aacgctaaca gcgcacttta tggcgctgtc
     1381 tggagggcgg caaaagatat gggctatcgc cgttgcatta catacaccag ggctgatgaa
     1441 tccggggcct ctcttcgggc agatggtttt gttcgtgtga aagaacttcc ttcaggaaaa
     1501 agctgggcag aatcaagtgt cgccctgaag agtacacgcg atccggttgg aagcggtggt
     1561 gttcctcgtg tgctccggga aatcaggaga atgagcacta ctgtcattca catcaaaggg
     1621 gaatgagata tggctattgc agagagaatg ggtatctcct cgcgcggcga tacttacgaa
     1681 atctggttgg gagcaaactg ttatcactac cgctctgatg gttatgcagt gcttctgaaa
     1741 gaaggcagga atcgtgctta tccatgtcgg gtgacgagtg aaacgattaa atctgcgctg
     1801 gcagcaattg ttgaattgca acagaaatta actgcggcag agaagaaaaa ggagtgtgag
     1861 aaccacgggg ttcttttgcc agctgagatg ttaattcgct gtctggaaga tcaaacaggg
     1921 ggacggtgga tccgtgcaca tgaaccagta atgctgatga aacgattaat cagtaacctg
     1981 aaaaaagcca gaccagaatg taccgttcct gatgaagcgc tggcatatct ccggcgtaac
     2041 tgtctggtga atatgggcga tatacccgtt accagcagac ggatgtctga cgtataacaa
     2101 aaaggaaaag tgtaatgaca ttacccgtag acagcctgat tgcgtacacg ctggaaaaaa
     2161 tgcaagagcg gatagtgcag aaaggccgac aacaggacgg aaaaatacaa agcggactgc
     2221 tgtttaccgg aaatgtgcat gatgccattc cgcgtcgact gcttctggat actcgacttt
     2281 ctccgctgga taagatggcg tggatgatga ttcggctgta tgcacagaat aatgaaggtg
     2341 cagttttccc cacatatgac gaactgcaac tgcagct
//
LOCUS       DAZGAP010000357         2354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGAP010000357 DAZGAP010000000
VERSION     DAZGAP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.359"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..756
                     /gene="tnpA"
                     /locus_tag="ACHLAM_005229"
     CDS             82..756
                     /gene="tnpA"
                     /locus_tag="ACHLAM_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO7856588.1"
                     /translation="MNSQTAKDITGFRSYLPDALRLRFDEQLSVRAIALQLGLSHSTI
                     HNLFQRFNASGITWPLPESLSVAQLDAILYANRKKELPDDPQISESTWRKERRTSYSR
                     EFKIRLVKQALQPGAVVARIAREHNINDNLLFNWKRLYEEGLLNDEDLQECQAVPVAI
                     TETLDPVKPVTNPFWRNKSDESQNDTTVGSPQCELHLKSGVVKLFSPLTPELLRVLIR
                     EMKGGI"
     gene            756..1103
                     /gene="tnpB"
                     /locus_tag="ACHLAM_005230"
     CDS             756..1103
                     /gene="tnpB"
                     /locus_tag="ACHLAM_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7856589.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFSKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1123..>2354
                     /locus_tag="ACHLAM_005231"
     CDS             1123..>2354
                     /locus_tag="ACHLAM_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO7856590.1"
                     /translation="MNNTLPDDIEQLKALLIAQQAVIVRLSGEITGYAREISSLRALV
                     AKLQRMLFGRSSEKSREKIEKKIARAETRITELQNRLGEAQLQLTSMAGETAPKTSDS
                     PVRKAFPATLPRDRQVISPAETECPVCSGKLKPLGESISEQLDIINTAFRVIETVRPK
                     LACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKFAEHLPLYRQSEIYARQGVELH
                     RNTMGLWVDIMGEQLRPLYDELKHYVLMPGKVHADDTPVNVLEPGQGKTRTGRLWVYV
                     RDDRNAGSTMPAAVWFSYSPDRKGIHPQQHLADYRGILQADAYAGYNALYESGQVTEA
                     ACMAHARRKIHDVHVRHPTTVTGEALRRIGALYAIESEIRGSPAEERLAVRKARTVPL
                     MQSLYEWLQGQMSTLSRHS"
BASE COUNT          600 a          638 c          630 g          486 t
ORIGIN      
        1 taagcgctcc gcctgtgacg tcttgtgatg gttgtcctgt ctggcaacaa tcgcgtccat
       61 ctatattgat ggacacgaac gatgaattct cagacagcaa aagatatcac cggctttcgc
      121 tcttatttgc cagacgcatt acgtctaaga ttcgacgagc aattatctgt ccgggctatc
      181 gccctgcagt taggtctcag tcattccacc attcataatc tctttcaacg atttaatgcc
      241 tccggtatta cttggccatt acctgaatca ctttcagtcg cccaactcga tgccatactt
      301 tatgccaata gaaagaagga attacctgat gatcctcaaa tcagcgaaag cacatggcga
      361 aaagaacgac gaacctccta cagtcgtgaa tttaaaatca ggctggtaaa acaggcatta
      421 cagcctggtg cagtcgttgc acggatcgcc agagagcaca atattaatga caatctgttg
      481 tttaactgga aaagactgta tgaagagggc ttactgaatg atgaggatct ccaggaatgc
      541 caggccgttc cggtggcaat cactgagact cttgaccccg tcaaaccggt gacaaatcct
      601 ttctggcgaa acaagtctga tgaaagccag aatgacacta ccgtaggctc gccacagtgt
      661 gagctgcatc ttaaatcagg tgtggtaaaa ctgttttccc ccctcactcc ggaacttctt
      721 agggtgctca ttcgcgaaat gaaagggggg atctgatgat aacgctgccg accggtacca
      781 aaatctggat catcgctggc atcacagata tgcgttgtgg cttcaacggc ctggcttcga
      841 aggtgcagaa cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc
      901 gcagtggcaa aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttctcca
      961 aacgcctgga acggggccgc ttcgtctggc cggtaacccg ggaagggaaa gtgcacctga
     1021 cgccagctca gttatccatg ctactggagg ggatcgcgtg gcaacatccc aaacggacag
     1081 aacggcctgg catccggata taacccgtga taaaacaggg gaatgaacaa cacactcccc
     1141 gacgacatcg agcaactgaa ggccctgctg atcgcacagc aggcggttat cgtccgtctg
     1201 tctggtgaaa taaccggcta tgcccgcgag atcagctcgc tcagagcgct ggtcgctaaa
     1261 ctgcagagaa tgttgttcgg tcgcagcagc gagaaaagcc gcgagaagat agaaaagaag
     1321 atcgcacggg cagaaacgcg tataaccgag ctccagaaca ggcttggtga ggcgcagttg
     1381 caactcacct caatggccgg agagacagcg ccgaaaacat cagactctcc cgtccgcaaa
     1441 gcatttccgg caacacttcc ccgtgacagg caggttatct ccccggcaga aaccgaatgc
     1501 cccgtctgca gcggcaaact gaaaccgctg ggagaaagca tctctgaaca actggatatc
     1561 atcaacaccg cgttcagggt aatcgaaacg gttcgcccaa aactggcctg cagccggtgc
     1621 gactgtatag ttcaggctcc gcagccacca aaacccatcg agcgcagtta cgccagtccg
     1681 gctctgctgg cccgcataat catggctaag ttcgccgagc atctgccgct gtaccgtcag
     1741 tcggaaatct atgcccgcca gggcgtggag ctgcaccgca atacgatggg gctctgggtt
     1801 gacatcatgg gagagcagct tcgcccgctg tatgatgaac tgaagcacta tgtgctgatg
     1861 ccgggtaaag tgcatgccga tgacacgccg gtaaatgtac tggagccggg tcagggtaaa
     1921 acccgtaccg gacggctgtg ggtctatgtt cgtgacgatc gcaacgccgg ttcgaccatg
     1981 ccggcagcgg tgtggttctc atactctccc gaccgcaaag gtatccaccc gcagcaacat
     2041 ctggcggact acagaggtat cctgcaggcc gatgcatatg cgggttacaa tgctctttac
     2101 gaaagcggtc aggtaaccga agcggcttgt atggcacatg cccgacgcaa gatccacgat
     2161 gtacatgtcc gccatccaac gacagtaacg ggagaagcac tccgtcgtat cggggcactg
     2221 tacgctatcg agtctgagat ccgcggcagt ccggcagaag agcgactggc ggtcagaaaa
     2281 gccagaacgg taccgctaat gcagtcgttg tatgagtggc tccaggggca gatgagcacg
     2341 ctgtcgcgtc actc
//
LOCUS       DAZGAP010000358         2344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGAP010000358 DAZGAP010000000
VERSION     DAZGAP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.360"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..>2344
                     /locus_tag="ACHLAM_005232"
     CDS             21..>2344
                     /locus_tag="ACHLAM_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7856591.1"
                     /translation="MSIPALNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARY
                     EVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAVILLSAPKGV
                     AIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCF"
BASE COUNT          576 a          646 c          710 g          412 t
ORIGIN      
        1 atctatacag ggaaataatg atgagtattc ctgccctaaa ctttgaccat tcacaccaca
       61 aactcaaaat ccgcggcctg caaagtccgg tggatgtgct gaccttcgaa ggccgcgaac
      121 aactgagtac cccgttccgc tacgacattc agtttaccag taccgataaa gccatcacgc
      181 cggagttggt gctgatgcag gacggcgcat tcagcctgac ggcaccgccg gtacagggaa
      241 tgccggtgca ggcccccctg cgcaccctgt atggcgtcat taccggcttt aaacatctgt
      301 catcgtcaca ggacgaagcc cgttacgaag tacggctgga gccgcgtatg gcgctgctgg
      361 cccgcagccg ccagaatgcc atctaccaga atctgacggt gccgcaaatc gtcgaaaaaa
      421 tcctgcgcga gcggcaccag atgcgcggtc aggacttcgt gtttaacctc aaaagcgaat
      481 acccggcccg cgaacaggtg atgcagtatg gcgaggacga cctgacgttt atttcacgcc
      541 tgctgtcgga agtgggcatc tggttccgct tcgccaccga cgcccggctg aaaattgagg
      601 ttgtcgagtt ttatgacgac cagagcggct atgagcgcgg gctgacgctg ccgcttcgtc
      661 atccttcagg gctgtttgat ggtgaaacgg aagccgtatg ggggctgaac accgcgtaca
      721 gcgtggtgga aaaaagcgtc accacgcggg actacaacta ccgcaccgcc acagcggaga
      781 tgatgacaga gcagcatgac gccaccgggg gcgataacac cacctacggc gaagcctatc
      841 actacgccga caacttcctg caaaaaggcg ataaagaggc agcggaaagc ggcgcgtttt
      901 atgcccgtat ccgccacgaa cgctacctga atgaacaggc cattctcaaa ggccagagca
      961 ccagttcact gctgatgccg gggctggaaa tcagggttca gggcgacgac gcgccagcgg
     1021 tgttccgcaa gggcgtactg attaccggcg tgacggccag cgcggcgcgt gaccgcagct
     1081 atgaactgac gtttaccgct atcccgtact cagaacgcta cggctaccgc ccggcgttta
     1141 tcccccgccc ggtcatggcc ggaacgctcc cggcgcgggt caccagcacg gtgaagaacg
     1201 atatctatgc ccatatcgac aaagacgggc gctaccgggt caacctcgat tttgaccgcg
     1261 atacgtggaa accgggttat gagagcctgt gggtacgcca gtcgcgcccg tatgcgggag
     1321 acacctacgg cctgcatctg ccgctgctgg cgggaacgga ggtcagcatc gcgtttgagg
     1381 aaggcaaccc ggaccgaccg tatatcgccg gggtaaagca cgactcggcg cacaccgacc
     1441 atgtgaccat ccagaactac aagcgtaacg tgctgcgcac cccagcaaac aacaaaatcc
     1501 gcctcgacga tgagcgcggc aaagagcata tcaaggtcag taccgagtac ggcggcaaga
     1561 gccagctaaa cctcggccat ctggtggatg ccgggaaaca gcagcgcggc gaaggctttg
     1621 aactgcgtac cgatatgtgg ggcgcagtgc gggcgaagaa aggcatcttt atcagcgccg
     1681 atgcgcagga caaggctcag ggtcaggtgc gggaaatggc tccggcaatg gcaatactgg
     1741 atggcgcaca gtcacagatg aagtcgctgt ccactgatgc gcagacggcg aatgccgatc
     1801 cggcagatct gtcatcacag atagcgctgt tgcagcagag tgtaaaagat ctgacgcagg
     1861 cggtgatcct gctaagcgca cccaaaggcg tggcgatcgc cagcggcgag cacctgcaac
     1921 tggcggcggc aaagaatgtg atagccaacg caggcaacaa cgccgatgtc ggcgtggtga
     1981 aaaatatgtt tatcggcgtg ggacaggcgc tgagcgtgtt tgtgcgtaaa gcgggcatca
     2041 agctgtttgc caataagggc gcaatctcgg tgcaggcgca gaacgacttg atggaactgt
     2101 tggcgaagaa ggaaatcatc attaccagta ccgaagatga aattaaaatc acggcgaaga
     2161 aaaagatcac ccttaacggc ggtggcagct acatccggct ggatgcctgt gggatagaag
     2221 cgggaacgcc gggagaatat aacgttaagg cgggatatta tgggcgtaag ccgaaggcga
     2281 agctgacgcc ggagctgatg gcgtttccgg tgattgaggg tggagactat tctcgctgtt
     2341 ttaa
//
LOCUS       DAZGAP010000359         2344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGAP010000359 DAZGAP010000000
VERSION     DAZGAP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.361"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(188..740)
                     /locus_tag="ACHLAM_005233"
                     /pseudo
     CDS             complement(188..740)
                     /locus_tag="ACHLAM_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            857..1762
                     /locus_tag="ACHLAM_005234"
     CDS             857..1762
                     /locus_tag="ACHLAM_005234"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO7856592.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGILNTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKK"
     gene            1807..>2344
                     /locus_tag="ACHLAM_005235"
     CDS             1807..>2344
                     /locus_tag="ACHLAM_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7856593.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSS"
BASE COUNT          682 a          399 c          575 g          688 t
ORIGIN      
        1 tcaactgcca gttccctgaa tgtttgattt gttcaataaa gcctgtttat caataaattt
       61 tctgacgaca gggaatggta tgtttttatt agtttttatg tgttggacaa caagacagaa
      121 taatgatgtc tgttttatgt atacgatata gccactgatt caacgcatta tcggctatat
      181 tgccagtctt atttagtgcc agattcattg tatcgaaggc cgctgccgtc agtagatggc
      241 tggtcagttc tatcagacag accattttta cctgaggata aagtgtctgg tttccaccat
      301 gtatttggcg agtgaagctt tatcgtgttc ttgcgtgtcc tgtgttcgtc agaacacgcc
      361 atcaatggtc agcagattca gatcgcacca gagcggatat ggcgtggtgt tatgccagag
      421 ctgcgctgtt tgagtgaaca cgcagcgtat ggctccgctt cctggccgct ggcgggcatg
      481 gagcacggcg ctactgcctg gcagtatgat gtccagccgg ttcacaatct gatgaaggtt
      541 ccttaagctc atgggccata ctgataattc accagaccat catttccaga ggaagacgcc
      601 gtttgtgtag cgtgacagtg tcggactctt cagggctgtg ggtaatcagt tccagggcaa
      661 gatagtcgca gagggttacg cagagaatag taactggaca ctggatcgat caaccgatcc
      721 ttaactgagc ggcatgacag ctttctgatg gatttttttg ataatcttca cattgtgctt
      781 cttttctgtt ggataatttt tgcacttggt ggtgtactag gtatcccatt atgcagttaa
      841 gtgaaggtga ttaaaaatgc aatgtgaatt taaaggggtc atatcagcat tacccactcc
      901 ttatgaccaa tcccagcaaa tcgatatgga gagtcttcga aaactgatcc gttttaatat
      961 cgaacaaaat attaagggat tgtatgttgg aggctctact ggagaagcat ttcttcagaa
     1021 tgttgcagag cgagaaaaaa ttcttgaaac agtagccgat gaatcagatg gaagactgac
     1081 acttatagcg catgtgggcg gaatcagtac ggcagaaagc gaggtacttg caaaagctgc
     1141 aaaaaaatac ggatatcatg ctatatctgc tgttactcca ttttattatc ctttttcatt
     1201 tgaagagcat tgtatccatt acaggaagat tattgattct gcagatggac ttccgatggt
     1261 agtttataat ataccggctc ttagcggagt gcgtttttct ttagatcaga taaatgagct
     1321 tgtcacaata cccggggttt gtgcattaaa acagacatcc ggtgatttat tccagatgga
     1381 acaaatcaaa aggaatcatc cagagcttgt gttatataat ggttacgatg aaatatttgc
     1441 atcagggtta attgcaggtg cagatggggg aatcggtagt acatataata taatgggatg
     1501 gcgttatctt gaaatatttg aagcagtcaa aaacaatgat gtgattaagg cgaaggaaat
     1561 gcaggtggcc tgtaaccagg ttattgacac tcttattcag agtggtgtcc tggcaggtat
     1621 aaaaactctt ctttactata tggggattct aaatactcct gtatgcagaa gtcctttttc
     1681 tccggtaaag gaaaagaatc tggatgtatt atcaaaactt gcagagcgac tgctcgaaga
     1741 acatgacaga aataaaaaat gaaaataatc tgaataattg caagattgtt atataggtga
     1801 aaatgaatga taacactggc tgttgatata ggaggaacga aaatttcagc ggcgttgata
     1861 tctgatgacg gttcattttt gctgaaaaaa cagatatcaa cacctcatga aagatgtcct
     1921 gacgaaatga caggggcttt acgtctactg gtttctgaaa tgaagggaac agcggagcga
     1981 tttgcagtag catcaacagg gattattaat aatggagtat tgacagctct gaatccagac
     2041 aatcttggag ggctgaaaga gtatcctctg aaaaatataa tggaagatat aactggttta
     2101 aatggaagtg tgataaatga tgcccaggca gccgcatggg cagaatatac agttttgcca
     2161 aaagaaattt gtgatatggt ttttataacc gtttctacgg gggttggagg gggaattgta
     2221 gttaacagga aattattaac aggtgtttct ggattagccg gacatgttgg gcatatattg
     2281 tccggagtaa cggatacaga atgtggatgt ggtcgtcggg gatgcgttga agcggtttca
     2341 tctg
//
LOCUS       DAZGAP010000360         2340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGAP010000360 DAZGAP010000000
VERSION     DAZGAP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.362"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..227
                     /locus_tag="ACHLAM_005236"
     CDS             <1..227
                     /locus_tag="ACHLAM_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7856594.1"
                     /translation="KKRNHPHADAIHVAGWVSLFTLHVIWPFLWIWATLYRPERGWGM
                     QNHDSSVVQLQQRIAGLEKQLADIKSSSAE"
     gene            233..1369
                     /locus_tag="ACHLAM_005237"
     CDS             233..1369
                     /locus_tag="ACHLAM_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7856595.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1366..>2340)
                     /locus_tag="ACHLAM_005238"
     CDS             complement(1366..>2340)
                     /locus_tag="ACHLAM_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7856596.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          579 a          556 c          599 g          606 t
ORIGIN      
        1 ccaaaaaacg taatcatcct catgccgacg ccattcatgt tgctggttgg gtgagtcttt
       61 ttacgttaca tgttatctgg ccatttttgt ggatttgggc cacgctttac cgcccggagc
      121 gtggatgggg aatgcaaaac catgactcct ccgttgtgca actacaacag cgtattgccg
      181 ggctggagaa acagctcgct gacatcaaat cctcttctgc cgagtaatac ttatggattt
      241 actgattatt ttaacctatg tggctttcgc atgggcaatg tttaagatct tcaaaattcc
      301 tgtaaataaa tggaccattc ccacagcggc cctgggaggc atatttattg tcagcggtct
      361 aattctgtta atgaactata accatccgta tacctttaaa gcgcaaaaag cggttatttc
      421 tattcctgtt gtcccacagg tgacaggcgt ggtgatcgaa gtgacggaca agaaaaatac
      481 gctgattaaa aaaggtgagg tgctatttcg actggacccg acgcgttatc aggctcgggt
      541 ggatcggctg atggcggata tcgttaccgc agaacataaa cagcgggcgt tgggcgcaga
      601 gttagatgag atggcggcga atactcagca ggcaaaggcc acgcgggata agttcgctaa
      661 agagtatcag cgttacgcac gtggcagcca ggcgaaagta aacccgtttt cagaacgcga
      721 tatcgatgtg gcgcggcaaa attatctggc gcaggaagcc tccgtaaaat cagcggcggc
      781 ggaacaaaaa cagatccaga gccagctgga tagcctggtg ttgggtgaac attctcaaat
      841 cgccagcctg aaagcgcagc tcgcggaagc aaaatataac cttgagcaga cgatagtgcg
      901 tgcgccgagc gatggttatg tgacccaggt gctgattcgt ccgggtacct atgccgcgtc
      961 gctgccgcta cgtccggtga tggtgtttat acccgatcag aaacgacaaa tcgtggcgca
     1021 gttccgtcag aactccttgc tgcgcctggc tcccggcgat gatgcggaag tggtatttaa
     1081 cgctctgcca ggcaaagtgt tcagcggtaa gctggcagcc attagtccag ctgttcccgg
     1141 tggagcttat cagtcgaccg gcaccttaca gacgttaaac acagcgccgg gttcagatgg
     1201 cgttatcgcg accattgaac tggatgagca tactgatttg agcgcattac cagacggtat
     1261 ttacgcccag gtggcggtct attctgatca tttcagccat gtctcggtga tgcgcaaagt
     1321 actgttacgc atgaccagct gggtgcatta cctttatctc gatcattaat cttcgctttc
     1381 cgccgcttgt tcaggccgca gttgctgcga ctgagcaagc tggtggcgaa tttcacgcag
     1441 catttcatcc agcacatact ggacccgcca gggctggtat tcccgtttac ggaagatagc
     1501 caccagatcc agccaccatt catacgggtg ctgaaagcag ggcaccagcg tgccgtcttg
     1561 caggtcgctc tgcacacttt tgtctggcgc aaacacaatc cccaggtgat ttcgtgccag
     1621 ctccagcgca gattgcgtgt tgtcgcaaac gtaatttccc ttcacgcgat aatcccgcac
     1681 ctctttactc ccggcgacgt tgaagcgcca gatattcgcg tcgtcgatca tcatcgagtc
     1741 gatcaaaata caggagtgat gttcgagttc gtcaggacgg ctaatgggat gtttctcaag
     1801 atagcgctgg ctggcataag cggttacggc atattgggta ataaaactgg caaccagcga
     1861 ttcatctttt ggttgggcgt agctgattaa aacatcacaa tcatcgggaa attcgacgcc
     1921 ttcataaaag gcattacgct caagattgca ggtttttagc gataaggtga tatcaccgat
     1981 atctttaatt ttatcgatta catgtttgga taaataggta attatgccgg ttggtgcata
     2041 gatagtgacc cgaccacgtt tctcatgctt ataatctgca ataaaattat taagctgctc
     2101 gtttctgtcc agcatgtcgt tgatatacgg taacagcacg gtgccaaaag gtgtgagcat
     2161 cagctgtcgg gtggttcggt caaagacttt taaacccact tttgattcaa aatcagcaag
     2221 atatttgctg acgttggcct gtgcgatgcc aagtacagtc gcggcatggc tgatattttc
     2281 actggcagcg attaccgaga taatttttaa ctcccggtac ttaagttgta attttgtcat
//
LOCUS       DAZGAP010000361         2325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGAP010000361 DAZGAP010000000
VERSION     DAZGAP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.363"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..398
                     /locus_tag="ACHLAM_005239"
     CDS             <1..398
                     /locus_tag="ACHLAM_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856597.1"
                     /translation="NKIREFDVTISQDNLNSVTQRLLPTPLAAVRARRVAFENPYRNK
                     VLSESLPAGNLLYRSALAFIQALIALVPSKKILDLDKITDALRRRYVHLEQLEYAELR
                     NALACALSTCYNRKYLIKVAKGKYLPVDD"
     gene            480..827
                     /locus_tag="ACHLAM_005240"
     CDS             480..827
                     /locus_tag="ACHLAM_005240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856598.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            1000..1164
                     /locus_tag="ACHLAM_005241"
     CDS             1000..1164
                     /locus_tag="ACHLAM_005241"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856599.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(1168..1635)
                     /locus_tag="ACHLAM_005242"
                     /pseudo
     CDS             complement(1168..1635)
                     /locus_tag="ACHLAM_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1699..1983
                     /locus_tag="ACHLAM_005243"
     CDS             1699..1983
                     /locus_tag="ACHLAM_005243"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7856600.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
     gene            complement(2200..>2325)
                     /locus_tag="ACHLAM_005244"
     CDS             complement(2200..>2325)
                     /locus_tag="ACHLAM_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7856601.1"
                     /translation="LLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          680 a          520 c          533 g          592 t
ORIGIN      
        1 ttaataaaat tcgcgagttt gatgtaacaa tatcccagga caatcttaat tcagttactc
       61 aacgattgtt acccacacca ttagctgcgg taagagcccg aagagttgca tttgaaaatc
      121 catatagaaa taaagtacta agtgaatcat tacctgctgg aaaccttctt tacagaagtg
      181 cattagcttt tatacaggcc ttgattgcat tagtcccatc aaagaaaata ctggacttgg
      241 ataagataac agatgctctg cgtaggcggt atgttcatct tgagcaatta gaatatgccg
      301 aattaagaaa tgctttagct tgtgcattgt caacttgtta taatagaaaa tatctcatta
      361 aagttgcaaa aggaaaatat ttacctgttg acgactaggt gcgtattacc gtgaaggaga
      421 tcggtgagta accccctgtg tcagcatagt tgtcgcctca attttttaag gagataacaa
      481 tgccaggaca accaatacca agtgagctca aagagaaagg actgatatgg cttttaccac
      541 catactgctg gtcacatcgt cagatagccc gtaagctaaa gatcagtgcc tcagttgtat
      601 caaaatggag atatgagtta gtggagcaag ggcttttacc tgaaaatgaa aagattacct
      661 ccgttgatgt tgaagactgg acaccggagc gtcgattctc agttgttctt gagacggcta
      721 cgctgtcaga aatggagctt gctgattatt accgacgcat gggattgttt gttgaacagg
      781 ttaaagaatg gcgggctatc tcaattcagg cgcatgaaaa aaattgacgg aaaatattcg
      841 aacagacagg gcattgcgtg acgcaaaaaa taagttacga gaactgtaga aagagcttaa
      901 gcgtaaagat aaagctctgg cggaagctgc ggccctgctt atattgaggg aaaagttcaa
      961 tgccctctgg gacaacagcg aggaagactg acccctctcc tggagcggcg taaaattatt
     1021 gcccttgtcc gcgaggccgt ctccaacggc gcccggaaaa gtcaggcttg taggtgtatc
     1081 ggtatttcaa ttcggacact tcagcgctgg actgatggca acgttattac cacggatgta
     1141 agcgtcaacg gagcaccgta ttgacgctta tttattggtg agtactacgt tccatggcag
     1201 gagttcatca actcggttgg aaggccattc cggcagtacg ctcaggataa ggcgcagata
     1261 cgcttccgga tcgataccgt tcagacggca ggtgccgatc agcccgtaca acagtgctcc
     1321 acgctcgccg ccgtgatcgc tgccaaagaa cataaagttt ttctttccga gacagactgc
     1381 acgaagcgct ctttccgcag cattattatc cgcctccgcc agaccgtcat cactgtaata
     1441 acagagggca tcccactgat tcagtacata gctgaacgct tcgcccagtc tggatttttt
     1501 cgacagcgta ccattcttct ccaccatcca ttcatgcagc gacgtcagta acactttgct
     1561 tcgctgctgc ctgacggaaa gacgctctga ctccggtaat ccccgtattt cgtaagcgta
     1621 aaccgaccgc cgtatgtagc cattaagcca gtattggtaa cgtaggtgcc caccttttca
     1681 aactagtgga cgcctgttat ggagcaaaaa acattatccg cagaaccccg cagatcattt
     1741 tcaaataatt ttaaacttca aatggttaaa ctggctttac aaccaagggt ttctgttgcc
     1801 cgtattgccc ggaagcatga tatcaatgat aatcttctgt tcaaatggct caggctctgg
     1861 ctgatcaaag ggcgtatatc acggcgtctt ccggtaagga actcttctgg cattggcgtt
     1921 gaattattac ctgtggagat gacgctggat gagcggcctt accccgcttt ttacgcgagg
     1981 tgattatgaa gatcaatgac tagccgtcga accgtgtgca tgaactgctg cactgaaact
     2041 tctcagctgt aaaataatcc ttacgctacg tacttcccgg agcgcttacg attaatcgga
     2101 tgcagtcctg actggaagaa agaaaaatgg gaaaaaataa gaggaattaa tatcctccac
     2161 gttcctgaaa tcctggagga tattaatgtc atcaaaatat tagcgaacga taatccctaa
     2221 caaaataccc agtgcgccaa agtcagcatc actgaaggtg gtgttagcaa aaccaatgtt
     2281 acccagcaca ggcaataaga atactggcag gaaggtaata agcaa
//
LOCUS       DAZGAP010000362         2311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGAP010000362 DAZGAP010000000
VERSION     DAZGAP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.364"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..691
                     /locus_tag="ACHLAM_005245"
     CDS             <1..691
                     /locus_tag="ACHLAM_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856602.1"
                     /translation="SPINIFIEKDKLSLVPGMVILVTRNIRELFRTYTDRVKVADINH
                     DIVSQYLEGGRRRMSSPQACFPHYLTSGIHQPVLAEALITNHGFPSAPSGDLSTMTGL
                     SCLSLFANDESLSSFLSRCLSNTSDKVRAIIQSDISVNWKLGVIALQLHMSESLLKKK
                     LKDEGNSFSRLLLEERMRGAVNMLCFQYGRGAIVAEKCGYSSLSYFISVFHRYYGVPP
                     DKYVSLHMVDF"
     gene            1769..>2311
                     /locus_tag="ACHLAM_005246"
     CDS             1769..>2311
                     /locus_tag="ACHLAM_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7856603.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGI"
BASE COUNT          560 a          536 c          634 g          581 t
ORIGIN      
        1 ttccccgatc aatattttta ttgagaaaga caaactttca cttgtgccgg gcatggtcat
       61 tctggtcacc agaaacatca gggagctttt tcgcacttac acagaccggg ttaaggttgc
      121 tgatataaat cacgatatag tcagtcaata tctggaaggt gggcgaaggc ggatgagctc
      181 cccacaggca tgttttccgc attatctcac ttctggcatt catcagcctg tactggcaga
      241 ggcgttaatt actaatcatg gatttcccag tgctccttct ggtgatttgt ccacgatgac
      301 agggctttca tgtctttctc tgtttgccaa cgatgagagc ctgtcatcat ttttgtcaag
      361 gtgtttaagc aatacaagcg ataaagtcag ggcaataatt cagagcgata tatccgtaaa
      421 ctggaagctt ggtgttattg cgttacaact gcatatgagc gaaagcttgt taaagaaaaa
      481 gcttaaagat gaaggaaaca gcttcagccg cctgttgctg gaagagagga tgcggggggc
      541 agtaaatatg ttatgctttc aatatggacg gggagcgata gtggcagaaa aatgcggtta
      601 ttcaagcctg tcttacttta tatcagtatt ccacaggtat tatggagtac ctcctgacaa
      661 atatgtgtct ctgcatatgg ttgactttta atttcatcct gctggcggat ggagtgctgg
      721 tggtcggcat tgtggtggtg ctttttcctt tggttaaaaa ggacatgcca ggtagttagc
      781 gggtatcagt catgcccaga aaattttaaa tgtctcacaa ttcagacggt tgacagttgt
      841 ctgttttgcg gggagtttgt taaaagaaac tggcatggtg aatccccctg agcggagggg
      901 cgactggtgc atcaggtgtt atctttgata cccaggaaag tgaacgcgcg ggttcggtgg
      961 caccgggctg aactcaccgg gaggcacccg gcactatgcg catgatgtac agatacgcgg
     1021 cttcagcccc tctccggagg ggcttttctg tgccttatgc atcacagttt ctggaaccct
     1081 gagtactaca gtatcagtca gggtgctata tttgcagatg tgatgaaagc ctgccggcag
     1141 gcagggcgta tcggaaatga cccagtaaga gaaaacgttg actcagatac cggtgctgag
     1201 ttaccgggaa accggcatca catgaccgct atccttcatg gctctctcag gagagccttt
     1261 ttactggaga aaaccgcttc cccgtaaaat gccgtgttgc tcacaattca gtcagttgaa
     1321 ggttgtctgc ccgactgaga atttgttaaa aaatttctgc atgatgaatc cccctgtgcg
     1381 gaggggcggc tggtgtacgg aatggtctct gatgatcgtt agcgagaatg acgcgggttc
     1441 ggtggcaccg ggctgaactc accgggaggc acccggcatc atgtaatgaa cggtacatag
     1501 cgctactctt cagcccctct ccggaggggc tttcttatgg ataaaaaaag cccgcatggt
     1561 gagatgcggg cagcaaggaa taaacaacaa aacgtgaagt aatcaatttt tcagctggcg
     1621 aataataccc gacagtaatc actctgcgca actgcgcggc ctttttcgta ttgcgggctg
     1681 tagtcttcct cctgccattg tcctgtaact tccggacttc agcccgcccc tcatctgact
     1741 cacaacatta tcccgaccgg gaggattcat ggcatttaaa cactatgacg tggtcagggc
     1801 ggcgtcgccg tcagaccttg cggagcgact gactcaaaaa ctgaaggagg ggtggcagcc
     1861 atttggcagt cctgtcgcca tcacgcctta taccctgatg caggccattg cggcggaagg
     1921 tgatgtcacc acacctgtgg tggtgaagcc gtcggatgga gaaggcacag ttatcagcgc
     1981 caccagcgac ccggagtatt actttgttgt ggttctggca gggcagtcaa acggcatgtc
     2041 gtatggtgaa ggccttccgc tgccggagac atatgaccgt ccggacccgc gtattaagca
     2101 gctggcgcgc cgaagtacgg tgacaccggg aggtgccgcc tgtaagtata acgacattat
     2161 tccggcggac cattgtctgc atgatgtgca ggacatgagc cgtcttaacc atccgaaagc
     2221 ggacctgtca aaggggcagt acggaaccgt ggggcagggg ctgcatatcg ccaaaaaact
     2281 gctgccgttt ataccggcga atgcgggcat t
//
LOCUS       DAZGAP010000363         2258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGAP010000363 DAZGAP010000000
VERSION     DAZGAP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.365"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..407
                     /locus_tag="ACHLAM_005247"
     CDS             129..407
                     /locus_tag="ACHLAM_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HFO7856604.1"
                     /translation="MNKMFSQWIDFDEMITPKIIKVIYWFFLFITVAGGVIGMIANPF
                     VGLLGLIMGIIGLRIGCEMIILLFNIYKQVKKIADAVPDKINEQAEDS"
     gene            1531..>2258
                     /locus_tag="ACHLAM_005248"
     CDS             1531..>2258
                     /locus_tag="ACHLAM_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO7856605.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGG"
BASE COUNT          616 a          417 c          558 g          667 t
ORIGIN      
        1 ctaaacataa ttctgatgtt ttagtacatg taacatattg acacattctt tatctgtttg
       61 tattttacgt gttagcagtg tttttatttt gacattgctg ttaacaaccg attgctggag
      121 aggtgaaaat gaataaaatg ttcagtcagt ggattgattt tgatgagatg attactccga
      181 agatcatcaa ggtaatatac tggttttttc tttttattac agtggccggt ggtgttatag
      241 gtatgatagc caatccgttt gtcggactgc ttggtcttat tatgggtatt attggtctga
      301 ggattgggtg tgaaatgata attcttcttt ttaatattta taagcaggtt aaaaagattg
      361 ctgatgcggt accggataaa ataaacgaac aggctgagga tagttaatta tctcagaata
      421 aacattgatg aatggtaata acagggactg ttaatactgt ccctgttatt ttttctgcag
      481 actgttttct gttcttcctg gttatgttct gagtgatgat gattgacgcc ttttatacgc
      541 tctgatatgc tcagaatgca ttatctgtcg cttcggcgac caccaatgct ccataagtgg
      601 tctgagagga gcgccttcgg gtggccacat cccgcttcgg tgggcaccaa tgttccgttg
      661 atctgagagg aacgccttcg ggtgatcaac acccccgtaa cctgaaaggg gatgtgcagc
      721 caggatgttt ctggtggtgg taaatctgcc cacctttgag agcagatatc gtcagtcatg
      781 atgatgaagg tttcttttaa ccgcgccctt tattacgggc aatacacagc agtggttgtg
      841 tgatccttat ctatccggtg atgccggaat gtttttactg cgtaatggcg gtattgactt
      901 cttttattct tttcatttgt ttgccagcac ttcctgcgac tgtgcgcgct gtataagacc
      961 atttgaaatt tggtcgctta cgcgtgtacc tggcgtctga agctgcgcgg cgtcactttc
     1021 gtcagcgtgt cacgatgaca aagtgacgca cgcgcgtctg cttaacgaac ccgcacgaac
     1081 tgccgtttca cgtgatgttt tttaacattt gaagaagtga cccggcgtga gagtgttttt
     1141 tcaacctctt ctgcaaattt acggacgatt ttgtgaataa cgtccataaa tggttttttg
     1201 cagaattaat tgttttttaa ggagttttat gtagtgagat gccgaatcgg cgataccact
     1261 tattagaggt ggtgtcgggg gcgacctgga gacaggtaca gcaaatgctg cagtcggtgt
     1321 gcaacaccga tttgttaccc ctcaatacct aaacaatctg cgctaccctg gcgcacaggc
     1381 agtcactacc cgaaggatcg caacgtttaa ccgtgtcgtc agggagaaat gcagatgagt
     1441 ttttctgttg tgttcacggc acaacagaaa aactcatctg ccgaacgtac agctgtttaa
     1501 ggtaaaaatt attatgtgca ggagaagttg atgtccagac gtgaaaaatt actgaaacag
     1561 gatctggttg ctcacattcg aaaagacaaa ggcagcttca agaagctgag tgattacacc
     1621 aggatcatgc accgctttgc tgaaagtctg gcaaaaatga atgtacagat cagttctgcc
     1681 gcgcaggtaa aagtccggca tattgagcta tatatgagaa gccggagtaa tgtttctggg
     1741 cgaacaaggg aaaatgaaat gtctgcaata cgggttatgt tacgtcaggc aggtaaatat
     1801 aagatggctg atcccgaaca tcctcgtctc agcaataaag cgttaggcat ttccggctcc
     1861 agtcgtaagg gaacaaaaaa attaatcacg gatgagcgtt ttcgggagat tcttcagcgt
     1921 gtggaagcaa aagataaagg ggtggctgct gttgttaaac tgtcacgtta cctcggtctg
     1981 agaaatgaag aagctatcca gtcggccaaa tcattacgaa cttggaaaaa agcgttacaa
     2041 cggggagatg aaaaaataag agtgattttt ggtacgaaag ggggacggga aagaatgaca
     2101 acggttgttg accgggataa cgttcttgat gctgttaatt atgcactcaa atatgccagt
     2161 gaacataatg gacgtcttat agacagagat aatcttaagt cggcaatgga ttattatctt
     2221 aatacattac gtcgttatgg agaactgaaa ggcggaga
//
LOCUS       DAZGAP010000364         2254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGAP010000364 DAZGAP010000000
VERSION     DAZGAP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.366"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(188..664)
                     /locus_tag="ACHLAM_005249"
     CDS             complement(188..664)
                     /locus_tag="ACHLAM_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO7856606.1"
                     /translation="MQIIIMTRDRYLEYGLMCMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            complement(727..933)
                     /locus_tag="ACHLAM_005250"
     CDS             complement(727..933)
                     /locus_tag="ACHLAM_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856607.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(1061..>2254)
                     /locus_tag="ACHLAM_005251"
     CDS             complement(1061..>2254)
                     /locus_tag="ACHLAM_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7856608.1"
                     /translation="NRHGITLSLISFLIAGAPAFAQDGSSWASPDTASASETGSTAVG
                     AWANAGKAGTALGSASYAHAPFSTSVGMSAYVNPKASNGVAVGSWASADALNSVALGT
                     GAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTRDTD
                     AATVGQLNRKADEVYSDVSGRIAAEALKARDYTDTVAAENRENIIRNTVAINRNTRGL
                     LSQRDLLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQEMA
                     TENRAAVSRNRQVGERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQSAA
                     LAGLFQPYSVGKFNATAAVGGYSDKQAVAVGMGYRFNEQAAAKAGTAFSDGDVSWNVG
                     VNWEF"
BASE COUNT          543 a          694 c          484 g          533 t
ORIGIN      
        1 atttcagata tccgggaaga agatattatc accacattat ctgtctgcat gccgctgata
       61 atgaatttac cgggattaat atactccttc attccctcca gaaaataact gttatcacaa
      121 ataaaatgca tacccgttcc acatgaaatt tatcacgcct ggccacataa cattaattat
      181 cccggattta ccggtctgcg gtgctgcaga gatatttaat actgacgccg cgcagaccat
      241 ttttctccat cgcattgtaa agatgcgcac ataccgtctt catgcttatc cccagctggt
      301 ctgccagaaa ggtaaaaccg tactcatgga aaacaactcc gaagaccacc accatctcgg
      361 tacgggtcag tggtcttgcc gtatgtccga acagccacgc cccacgacgg atggcctgcc
      421 tgatatctgt caggcatctc acagaggaaa caggaatgac aagaaaacga cgcccacaga
      481 acatacagta tgtaagcctt tccagattac ggtcacagag aatcacataa ctgtcttcgg
      541 gaagtggcag acggcgcttt ccggcatcaa acagttcacg gcctgtcgtg agcctgtatc
      601 cgttcagcat acacatcagt ccatattcca gatatctgtc acgggtcata ataataatct
      661 gcatactctc agacccttat ctcccggata ccggctccgt ttaccattac cggtataact
      721 gactcatcat tcataacgga aactgtctgc cgcatacatc agcgcccgct cttccagctc
      781 tgacagactc agctctttca gttccccttc tccttcagca agaagacaac attcaaaaac
      841 agctgcatcg tcgcattcca gacgaacctg caccctgtca gcccgaccgt ttacagtaaa
      901 tacctgcact gaacttatgc gatagttcag catatacact ctccctctca gctcctcagg
      961 aataccccgg aaccgtcagt catgccaccg gggagaaaag caggcagcga atcatgaccg
     1021 catacagaac gctgcccgcc ccggtgactc agacccgggt tcagaactcc cagttgacac
     1081 ccacattcca ggaaacatca ccgtcactga acgcagtacc tgcctttgcc gcagcctgtt
     1141 cgttaaagcg atatcccatg cccaccgcaa cagcctgctt gtcactgtac ccgcccacag
     1201 ccgctgtggc gttaaattta cccacactgt atggctggaa aagaccggcc agcgctgctg
     1261 actgggctgc tgcccgcttc atttcctcat gattctcatt aatctgacgc tgctgactgt
     1321 tcatacgacg ctcatgcgcc tgcagggtac gtgcctgaga agcaatggcg cgactgttgc
     1381 gttcacccac ctgacggtta cggctgaccg cagcacggtt ttctgtggcc atttcctgtg
     1441 tacgtaccag cccttcctgt acagtaattc gttcaccggt tccctcaccg cgcgtcacat
     1501 aaccatgact gtccacagca acggtacttc cggtatttat ctgctgttgc tggctgttca
     1561 ggcgctcttc atgcgtctcc agcaggtctc tctgggaaag cagtccccgg gtgttacggt
     1621 ttatggccac cgtgttccgg ataatattct ccctgttctc tgcagcaacc gtatcggtat
     1681 agtcacgcgc cttaagtgct tccgctgcaa tccgtccact gacgtcactg tacacctcat
     1741 ccgccttcct gttcagttgc ccgacagtgg ctgcatccgt atcccgtgta ccatcactga
     1801 caccaccgag aacccgggtt ttatccggta cacgcggact ggccgggttt gtgacacctg
     1861 agtcatatcc gatgtagact tcattttcac gggttgtacc ggactgctga ccaagagcca
     1921 ctgaatacct gtgcattgcc tgtgcacccg tacccagtgc tactgaattt aaagcatctg
     1981 cagatgccca gctacctact gccacaccat tagacgcctt cggatttacg taagctgaca
     2041 tccccacgga tgtggaaaac ggcgcatgcg catacgatgc agagccaaga gctgtccctg
     2101 ccttcccggc attcgcccat gcccccactg ccgtggagcc agtctctgat gcagaggctg
     2161 tgtccggaga cgcccaggaa ctgccatcct gcgcaaacgc cggagcaccg gcaatcagaa
     2221 aggaaataag cgataaagta ataccatgtc gatt
//
LOCUS       DAZGAP010000365         2246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGAP010000365 DAZGAP010000000
VERSION     DAZGAP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.367"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1281
                     /locus_tag="ACHLAM_005252"
     CDS             <1..1281
                     /locus_tag="ACHLAM_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7856609.1"
                     /translation="QMRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAA
                     KANFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADR
                     GAGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKIISAYNSQGTLSAEETKQLEE
                     VKKERADMFATYMATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDL
                     QTVTEKADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAE
                     EHKLLISENSKQISTNTLGIRENTQRIRDEGQARIRGDRETLRAATGYTDMRVNGLER
                     NTNKRFAQLKEDIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAA
                     GFHFNTSENSAVKVNAAWDTENGAGVSAGMALGW"
     gene            complement(1711..2244)
                     /gene="dolP"
                     /locus_tag="ACHLAM_005253"
     CDS             complement(1711..2244)
                     /gene="dolP"
                     /locus_tag="ACHLAM_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7856610.1"
                     /translation="MLQGCVAAAVVGTAAVGTKAATDPRSVGAQVDDEVLELRVSSAL
                     SKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVDGVQEVYNEIRQGTP
                     VSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLGLVTEQEGRAAADIA
                     SRVSGVKRVTTAFSCIR"
BASE COUNT          642 a          518 c          560 g          526 t
ORIGIN      
        1 caaatgcgta aaatcagtgt cagttatata tgcaaatcat tatttctcta ctgctcagct
       61 tctttacctg tcttatctgc aaatggtatt cctgataaaa tgatcgtgga acttggtgat
      121 aacagtgcgg caaaagctaa tttcgctgtt gctgttggtc gatattcaca ggcaaatgca
      181 gaaaaaagta tttctttagg tgcatcagca agagctattg aaaataactc taccgcgtta
      241 ggagcaaacg caacaacatt gaaaccattc tctgtcgctc tgggcgcagg aagtgttgct
      301 gatcgtggcg ctggtattga agcatatatg ccagagacaa agcgctggaa tccaggggat
      361 gtagtaaaat ccaggatgtc tgacaagcag cttaacaggc ttatggaaat cgataagata
      421 atatccgctt ataattcaca agggacgctg tctgctgaag agactaaaca actggaggaa
      481 gtgaaaaaag agcgggctga tatgtttgcg acatatatgg caacgcacga tgctgtatct
      541 gtaggtgttg gtgcaacgac aacaggacga catgctgtta caagacagat aactagcgtt
      601 gcagcaggtt ctgtcgatac ggatgctgtt aacgttgcac agctcaggga tttgcagact
      661 gtgacagaaa aggcagatgc agcgcttgat aaacgcataa caaacaacac cagtgcgata
      721 actaaaaatc tgcaggtggt taacagtaat aaagaggaaa tcagtaacat taataaaacg
      781 acaaaaattc tggatgaaag agtgacagcc aatacacaga cagcagaaga acataagctg
      841 cttatttcgg agaacagcaa acaaatcagt accaacactc ttggtatcag agagaataca
      901 caacggatcc gggatgaagg acaggctcga atccggggag acagggaaac actgcgtgca
      961 gcgaccggtt ataccgacat gcgggtaaac gggctggagc gtaatacgaa taaacgtttt
     1021 gcgcagctta aagaggatat agacaaaaac agaaaacgtg cagatgcagg tattgcaggt
     1081 gcgatggcaa tgacggggat accgtccgtc cccgggaaaa aggtctcatt tggtatggcc
     1141 atggccggat accggagtga aggtgctgtt gctgccggct tccattttaa tacaagtgaa
     1201 aacagtgctg ttaaagtgaa tgcagcctgg gacactgaaa atggagctgg cgtttcagcc
     1261 gggatggctc tgggatggta atgcttccgg gccagaagcc gttcgcagat attgttatcc
     1321 aggggacact gcaccaccat tcaggaagcg attcagttta tcgcatcggc tcaggatgta
     1381 attaatacgg acaatctggc tcctgcaccg gagccagacg tacacgtgtc aacgccacgc
     1441 tctttgacct gtatggataa aagctcagat tatttaccag cagcgacatc cggagcgcac
     1501 cgggatttta tctgtgagtg aacatacccg ggcaaaaaaa tcccccgaat tcaggggggt
     1561 gtcaaagagg aattatgtat attggttatt ataatgagca cgtctccggt attccgcgac
     1621 gaatgttcac gtggtggcat gaacggcccg gaaacgtgct cttatgattt ttaacgtaaa
     1681 tttgcactaa aggcaactca gatacggcct tcaccgtata caggagaatg ccgtcgtgac
     1741 ccgcttcacg ccactcaccc tactggcaat atctgctgcc gctcgccctt cctgctctgt
     1801 gaccaggccc agtaaaaaga cttcgctgtt ttctgtcacc accttgacac tggatgattt
     1861 gacacgggca cttgtgagta actgagaacg aatttttgtc gtgagccagg catcacctgc
     1921 cattatgacc ggactgacgg gagtcccctg ccggatttca ttatagacct cctgaacacc
     1981 atcgacaccg agtgctatct gacgggcacg ggcagataat tcacggtccg gagactgacc
     2041 gatcagcagc acgcgtccct gatatgcaat ctcactgatg cgtgcttctt ttctgatttg
     2101 ttcatctttt gaaagtgcac tgctgactct cagctccagc acttcatcat ccacctgcgc
     2161 tccgacactg cgtgggtcgg ttgctgcctt tgtcccgaca gcagctgtac ccaccacagc
     2221 ggcagccacg cacccctgta acaata
//
LOCUS       DAZGAP010000366         2243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGAP010000366 DAZGAP010000000
VERSION     DAZGAP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.368"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2049
                     /locus_tag="ACHLAM_005254"
     CDS             <1..2049
                     /locus_tag="ACHLAM_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO7856611.1"
                     /translation="VINDLLKNDYKISLSPDTTYRELNKAAQRSITAPDRIGEGKTWV
                     YQRDTMVERGDNRGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYELAADVYNIN
                     VTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQ
                     QIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGD
                     LQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIESGIPG
                     YWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFETSKDNISKDEWENLHALKEKRLN
                     GMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLTIYNER
                     LHNTNLFKPIYHEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKTIKAF
                     SFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQLAMAY
                     MAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYI
                     DKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPG
                     IYKNMGSNSVNIIINNTRKIIPPGKAFMLRGGTLNINIPGRSALLLGKTGEPPNYLYL
                     "
BASE COUNT          755 a          448 c          416 g          624 t
ORIGIN      
        1 gtcattaatg atctgttaaa aaacgattat aaaatttccc tttctccaga tacaacttac
       61 cgagagctga ataaagctgc acagcgtagc attacagcgc cagacaggat aggagaaggg
      121 aaaacatggg tttatcaacg agatacaatg gttgaaagag gtgataacag aggcgttcat
      181 cagtatggtc cagctgaaca tttcactcac attatatctg acaaaccatc cccaaaagat
      241 aagtatgttg catatgctat taacattcct gactatgagc tagcagccga tgtatataat
      301 attaacgtga cgtcaccttc cggacagcaa gaaacattta aaatactaat caatccagaa
      361 catctacggc aaacacttga gcgtaaatct cttactgctg ttcagaaatc acaatgtgaa
      421 atcatcaccc ccaaaaaacc tggcgaagcg attcttcatg cttttaatgc cacctaccag
      481 caaatcaggg aaaatatgtc tgagtttgca cgttcccatt atgggtatat acaaattcct
      541 ccagtgacaa ctttccgcgc cgacggacca gaaactcccg aagaagaaaa aggttactgg
      601 tttcacgctt atcaacccga agatctttgt accatccata atccaatggg agatttgcag
      661 gattttatcg cattggttaa agatgctaaa aaatttggta tcgatatcat tcctgattat
      721 acctttaact ttatgggaat tgggggtagt ggtaaaaatg acctagatta tccctctgct
      781 gatatacgag cgaagatcag taaagatata gaaagtggta tccctggcta ttggcaaggt
      841 caggttttga ttccatttac tatagatcca gtaacaaaag aacgtaaaca aatccatcca
      901 gaagatatac atctcactgc aaaagacttt gaaacaagta aagataacat ctctaaggat
      961 gaatgggaaa acctccatgc attaaaagaa aagcgtttaa atggaatgcc taaaacaacc
     1021 cccaaaagtg accaggttat tatgttgcaa aatcaatacg ttcgtgaaat gcgaaaatat
     1081 ggcgtacgag gtttacgtta cgatgcagca aaacactcaa aacatgaaca aatagaaaga
     1141 tcaataactc caccgcttac aatttataat gagcgattac acaatactaa cttatttaag
     1201 ccaatatatc atgaaaaagc cgttatgaat tacatggaat acctggtaac ttgtcagttg
     1261 gatgaagaac aaatgtcatc gctgctttat gaaagagatg atttaagcgc cattgatttt
     1321 tcattgctca tgaagacgat aaaagccttt tcatttggtg gcgatctcca aacccttgca
     1381 tcaaaaccgg gttcaacaat ctcaagcatc ccgtcaaaaa gacggatatt gattaacatt
     1441 aaccacgatt ttcctaacaa tggcaatctt ttcaatgact ttctatttaa ccatcaacaa
     1501 gatgaacaat tagcaatggc atatatggcc gctctcccgt tcagcaggcc tttagtttac
     1561 tgggatggcc aagtattaaa atcaacgact gaaattaaaa attatgatgg gtcgacgcgt
     1621 gtcggcggtg aggcgtggct taataaaggt tgctctacct atcagcagct ctacaatgaa
     1681 ttccacgcat tatatataga taaagcagga atatggagcg catttgaggg tgtatttgca
     1741 actaaaaacg ttctggcctt tagtcgtggg gattctgtga acattaatca ctctcctcat
     1801 gatggactag ttataataaa taaaggaaac gaagaagttg aaggtacctg gcctaacaaa
     1861 ttgcaacctg gaatatataa aaacatgggg agtaatagcg ttaacattat tattaataat
     1921 acccgaaaaa ttatcccccc tggtaaagca tttatgctta gaggcggaac tctaaatatc
     1981 aatattcctg ggcgtagcgc tcttctttta gggaaaactg gagaaccgcc gaactatctc
     2041 tatttgtaat ttaatgttat atctgccccg ctaaaacggg gcagataata tgtttagttt
     2101 actaacggtc attttgcagt gaagccattt accgtttttt atcgaccaga taatctgttc
     2161 tctgatggta atccacccct aacctgttgc tttagttatt catttcctgt ctcactttgc
     2221 cttaataacc tacgtcaaat gtt
//
LOCUS       DAZGAP010000367         2235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGAP010000367 DAZGAP010000000
VERSION     DAZGAP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.369"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(110..1108)
                     /locus_tag="ACHLAM_005255"
     CDS             complement(110..1108)
                     /locus_tag="ACHLAM_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO7856612.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTSMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
BASE COUNT          541 a          654 c          542 g          498 t
ORIGIN      
        1 aaaaaaaacg ggcaaaacct tgcccgtttc tgaaaatcac gttagaacgc ttctgagcgc
       61 gtttctgaat attctcccag tgcatgaata gttcgcgcct gaaacgcgct caatcctcgc
      121 cacttagctg acgtttatct ttcgcccgat cgcgctggta ttttttgaca accggcaccc
      181 acgaaaacgc cagacgggaa ccgtcatctg taccctctgc catgccatcc gccatgatta
      241 aatcctcgtt cgtttcccgc tccagttgca gaacgttctt aagcgctccc cccgtagcaa
      301 caaaacgacg cttgtgtacc tgccgtgtta attcgaggaa ccattcagga tcagccagca
      361 tatcagcagg ctttgtggaa tatttcagag tttcaaccac cgcgccgcgc agctgttccg
      421 caacaacaca aggtaaagat tcatcgtctt ttggtttccg tgacttcacc actctgatat
      481 ccacattcgg gtcatacgcc acccgcagac aatcacgcca cagttccacc caacgggcat
      541 gtttcacata ctcacggccg gacaacatcg aagggggaac catcagcaga gtgtgaaaat
      601 ggggatgtgc gctaccatcc ttgccgcgcg ttacttccgt ggtacgaacc cagcccaaca
      661 catggcgaaa ttccttacgc tctttcaacc gcttaaaccc cgcattcatc gaggtaagcg
      721 tctcaccaag cgcgtcaatg gaacagttgc gcactgtcag tgtgagaaac aaccagcgcg
      781 cgccgggatg ttccgtcaca atctttggca gggactgata gaaacgggct tgccacatca
      841 tggaacgcct ccactgacag atcggacagt gccggacgcg acagaaccgt gcatcacgca
      901 accgcaggcg cgtttctccg gtctctgtgt ttgttgacca cccaaaccgc aaaataccac
      961 tacaagcccc gacgcgttcg ccgtagtgct caaatccggc agcacgcaga taaataccgc
     1021 ccacatcgtc ggaaaagctt cggtgttcat cccacggttt atctgctgga gagtaatccg
     1081 tcagattctt aagttcagga tttttcatgt ctgccgtccc cttgcgagcg acagacgaaa
     1141 ttgcgtgtta tcattcctgt atcacctgta ccaattgtcg tcaaatcagt tcatgttcta
     1201 ggtggtcgcg cccctggagt ggtggaagac tcgcaggggc taatttataa ccactcccga
     1261 caaaatactc tcaaacctat ttacacgcaa atccgcgctc aggccagata tggcaaggga
     1321 tagaggcgat ccaaaaaatc gcatttatcc cttaagtata aatacaagga aacgcgcgcg
     1381 aagatagcag caaaatcgcc tgttttttgt tcgattcccc gagggcaaaa acaacaaccg
     1441 cgaaagcgtt ttcccgacaa aacacacaca ggcttttcca ccgattttgt gaacagcaaa
     1501 aaccgtcttg caggtggttg gtctcactcc gtgacaccaa ccaccatccc cagtgttttt
     1561 cccgtgccgt cgtcaatcac aaaatcggaa ccccggaggg gcccccgcga ttttctgttt
     1621 gacgagttcg tgaacggtga gcgggacgcg gaattttttc gcgtgccgtg aggcgttggc
     1681 ggggtgtcgg ggtgcagccc tgaccaggtg gggaatgtct gagtgcgcgt gcgcggtccg
     1741 acattcccac atcctgtccc gatttccgcg ggtttgtgtt accggaacgc cgacgcaata
     1801 tcggcagttt ttgcgccgtt tcgccctaca cggaagcgcg cagttctggc aaccggacgg
     1861 caaatcattt tgccgttttc gtgccgcagc cgttccgcgc gatcgacaaa ctggcagtcg
     1921 ggggaacctc acgaagtgca cgaagtgcgc acttatacaa acttcacttc gtttcgtttg
     1981 ttgtgcggtc tgcgacgctc aaaaaaccga gaaacggcgg gaattttcgg gacaggatgt
     2041 gtaatcgtat aaccgcgcac gcacgccgat acgatcacct cctggtcagg gctgcacccc
     2101 gacaccctcg acaacctgcc cgggacacgt taaaacgaca tctgcgcgtt tctggtgagc
     2161 gaaaagctgt cagccgtgga ttgccgtagc agagacccgg aaacgcgctt aaaacgcttc
     2221 tgagcgcaga gaaac
//
LOCUS       DAZGAP010000368         2228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGAP010000368 DAZGAP010000000
VERSION     DAZGAP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.370"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2047
                     /locus_tag="ACHLAM_005256"
     CDS             <1..2047
                     /locus_tag="ACHLAM_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO7856613.1"
                     /translation="KSRKSVKSVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGAGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
     gene            2080..>2228
                     /locus_tag="ACHLAM_005257"
     CDS             2080..>2228
                     /locus_tag="ACHLAM_005257"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7856614.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NNGIKG"
BASE COUNT          696 a          423 c          538 g          571 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacagctg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcgca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggtag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat gatacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcattcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccaccgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctggcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag ataccagacc gacactggag gattttaaaa agacggttgg
     1981 cgactatctt gcgtgggagg tgtcaaaact tcttcagggg cgtgatgcgt cagaaaaaaa
     2041 gcagtaaact cccgtcttgc agagcttgag cgggagttta ttaaatctgg tggtaaatgg
     2101 gaagaataca caatcgaaag attgttcagt attcagacac ctaaaaagaa atttaatgcc
     2161 aataaattaa catttaatgg caactatcct tatgttgcca gaggaagtaa caataacggc
     2221 ataaaagg
//
LOCUS       DAZGAP010000369         2209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGAP010000369 DAZGAP010000000
VERSION     DAZGAP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.371"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            358..2139
                     /gene="tssD"
                     /locus_tag="ACHLAM_005258"
     CDS             358..2139
                     /gene="tssD"
                     /locus_tag="ACHLAM_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO7856615.1"
                     /translation="MSDIVYLRIIGEKQGYISSGCGTYASVGNCWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECITVDYDYILCKHLIVNTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKEKGCTDAGTAEEPAENFGQVA
                     IFAQPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLDQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDLRKLPGVVIGRGVPLSLGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSKDSVLLAQFIKSNQNNVSQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAI
                     EHGGGVYDIDNFRIVTPRLHDEIHYRR"
     gene            2139..>2209
                     /locus_tag="ACHLAM_005259"
     CDS             2139..>2209
                     /locus_tag="ACHLAM_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO7856616.1"
                     /translation="MYMRDLKDKIYFYTEGEFLEMLE"
BASE COUNT          622 a          452 c          524 g          611 t
ORIGIN      
        1 tgtagatgcg ttgatttttt ggataaaaaa aaagcaattg taagtttttc atcacattta
       61 atagatactt taagtctgat aacatactgt tttaactagc tatatgccgg atttgtctgc
      121 ctgctgaaaa ctatgcctta gccaccagca caataatctt acaaaaggtc atataagaat
      181 aaatagatgt taataataca atgggtttgt aacgcgagta tttaaatata atattgcgag
      241 ctaagtgaaa acgttaagta attgaaataa tctttcatta aaagcgtttc ttattgatcg
      301 agaattaagt tttctattta atgcaaataa gttaaggata aactggaatg aaataaagtg
      361 agcgatattg tttacctgag aataatcggt gagaagcagg gctatatttc ttcaggctgt
      421 gggacgtatg cgtcggttgg taattgctgg caggttggcc atgaagatga aatttttgcc
      481 tttgcactca ccaacgccat taccagtact ggtaaaggcg ttaatctaca ggggctacaa
      541 ttttgcaaac tcattgataa aagctcaccg ctactgtcta atgccatcaa tcagaatgag
      601 cggttattta ttgaaatcga tttgtatcgt ataaataaaa gcgggcgctg ggaacggtat
      661 tattatattc agctaagaaa tgcttcatta actgctattc atgtaaacat ttctgacaat
      721 aatcttccta ccgaatgtat aactgtcgat tatgactaca tattatgtaa acatctaata
      781 gtcaatacgg aatttgactg gttggccttt cctgctggct ataatagctt atttattccg
      841 cctaaaagcc cacctgccag taatcttaag cctgaacctc taccagttgt taaccttcca
      901 ctctcttcac cagcggttaa cccggtctat gcaaaatcct gtctgaagga gaagggatgt
      961 acagatgctg gaacggcaga agaacccgct gaaaacttcg gtcaagtagc gatttttgct
     1021 cagccagtgg ttgatgactg ctgtggatac caccatcccg aggctaacga tgtcgggcaa
     1081 cccgcagaag ctcaaaccat gctactgttt ccgggtagtg tgttggcggc tcaaatatgg
     1141 ggaaaatggt cgctcagtgg catactcagt gcaacccgcg gctcttacat cggtgcgttg
     1201 gcatctgctt tgtatattcc ctctgcgggc gagggcagtg ctcgcgtgcc cggacgtgat
     1261 gagttctggt atgaggaaga actgcggcag aaagcactag caggcagtac cgccaccacc
     1321 cgggtacgtt ttttctgggg aactgacatt cacggcaagc ctcaggtgta tggtgttcat
     1381 acgggtgaag gtacgccgta tgaaaacgtc cgcgtggcga acatgcagtg gaacgagcag
     1441 acgcagcgtt atgaatttac ccccgctcac gatgtcgatg gccccctgat tacctggacg
     1501 ccggaaaatc cggaacatgg gaatgttccg ggccataccg gtaacgacag gccgccgctg
     1561 gatcagccca ccattctggt gacgccgatt ccggacggca ccgataccta taccacgccg
     1621 ccattcccgg ttcctgatcc gaaagaattc aacgattata ttctggtttt tccggcggga
     1681 tccggtatta agcccatcta tgtttacctg aaggaggatc tgcgaaagct gcctggtgtt
     1741 gtaatagggc gcggcgtccc gctttcacta ggaactcgct ggctggatat gtcggtatcc
     1801 aataacggca acggcgcacc aatcccggcg catattgctg ataaattgcg cggacgggag
     1861 tttaaaacct ttgatgagtt tcgcgaggcg ctgtggctgg aggtgagtaa agactcagtg
     1921 ttattggcgc agtttataaa aagtaatcag aataacgtta gtcaaggata ttccccctat
     1981 gtccctgagg aggggtatta ttacggacca aatgagatag ttaagaaatt ccagatacat
     2041 catgtagttg ctatagaaca tggtggtgga gtgtatgata ttgataattt taggattgtt
     2101 acgccccgac tacatgatga aattcactac aggagataat gtatatgcgt gatttaaaag
     2161 ataaaatata tttttacaca gaaggtgaat ttcttgaaat gcttgagga
//
LOCUS       DAZGAP010000370         2187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGAP010000370 DAZGAP010000000
VERSION     DAZGAP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.372"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..1316
                     /locus_tag="ACHLAM_005260"
     CDS             111..1316
                     /locus_tag="ACHLAM_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7856617.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFKKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFKTKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHALYLDGRREMMQWYADYIDSLSSH
                     ESQCQGPLY"
     gene            1724..1990
                     /locus_tag="ACHLAM_005261"
                     /pseudo
     CDS             1724..1990
                     /locus_tag="ACHLAM_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          672 a          471 c          451 g          593 t
ORIGIN      
        1 cggctttcaa caacatcttt gttatacaag gggtgcattg ggggtatcag gttaattact
       61 ggcagaaatc cccctcagga acatgaattc attaaaagcc ggaggatgtc atggcattaa
      121 ccgatattaa agtgaaaacc gcaaaaccaa aagaaaagcc ctataaactt gctgatggcg
      181 gcggtatgta tctactgatt aacactaatg gttctaaata ctggcgtatg aagtaccgct
      241 ttgctggtaa agaaaagatg ctttctattg gcgtataccc cgatgtaacg ctagccgacg
      301 ctcgtgaaaa acgcagcgaa gccagaaaaa tcctcgctgc tggaggcgat ccaggggtgg
      361 caaaaaaaga agaaaaaatc gcgcagcaaa tgagtttgaa aaatacgttc gaggccattg
      421 ctcgcgaatg gcatcagtca aaagccgatc ggtggtcatt acgctatcgt gatgaaatca
      481 ttgatacctt taaaaaagac atttttccct acattggcaa acgccctatc gccgaaatca
      541 aaccaatgga gttactggaa gcactgcgta aaatggaaaa acgtggagct ctggagaaaa
      601 tgcgcaaagt ccgccagcgc tgtggtgagg tgttccgcta tgcaattgtt actggtcggg
      661 cagactacaa ccctgctccc gatcttgcct gtgctttagc tacaccgaaa aaagtacatt
      721 ttccctttct tactgctaat gaacttcccc acttcctcaa cgatctggcg ggttataccg
      781 gaagtatcat cactaaaaca gctactcaga tcattatgtt aaccggtgta cgaacacaag
      841 aattacgttt tgcacgctgg gaggatatcg attttaagac aaaattatgg gagattccgg
      901 cagaagtaat gaagatgaag cgacctcata ttgtgccgct ctctgagcag gtcattgccc
      961 tatttaagca acttgaaccc atcacaaaac atcatcctct ggtctttatc ggtaggaacg
     1021 atcctcgcaa gccaattagt aaggaaagca ttaaccaagt cattgaatta ctgggatata
     1081 agggaagact cacagggcac ggtttcagac acaccatgag taccattcta cacgaacagg
     1141 gctttaattc cgcttggatt gaaacgcagt tagctcatgt agataaaaac gctatcaggg
     1201 ggacctataa tcatgccctg tatctagatg gtcgccgtga aatgatgcaa tggtacgctg
     1261 attacattga ttcgctttcc agccacgaga gtcagtgtca aggccccttg tactgatatt
     1321 ttctctcaat tcacaaaatt cctccggcct ccaggtctgg aggaattttg ttttcatgtt
     1381 ttatttttag atattttttg acaaaatatt tatattgcat ctgcaaacaa tctcagaaca
     1441 tcacaccgct atagaccgct aacaattttg atgcctgtaa tttcaatcat catcctgtcg
     1501 ttatttcagc accatcacac caaaaatcaa ctaacatgtt gtcttagccg actattcaca
     1561 atgcattgga atgcagataa agataaattt ttatccacat gaagaattac tgtcgatgat
     1621 attgtaaaca atattcatcc cgggcaataa caagacttga tacacaacat cacgactttt
     1681 atgcttttca gattgtgtcc catccgtttt acaggctcag aaaatgaacg acagattacg
     1741 ctttgatgtg aacgataacc aaggccgttt cgtatttccg aataccaggt ttggcccatt
     1801 actggaggag tttgaagaag tgcatgacgc atacggcact gatgaaatca gcgagacgag
     1861 ttatatcaac aaactcaggt agcttgccca acgggagcct gactttattg atatacacgc
     1921 tcatctggca tatgcatttc tggagcagcc aaggtgggca atatttttat ttatttctgt
     1981 gaaaagcctg ctaaagatcc catgcttcta tattaatgac tggaacaata ctaggaagca
     2041 cattaagaaa aaataaagga agccgatggc taccacgttt atcgtttaga agcgtgagct
     2101 cttcattatt aagccggaca cttacaaaat gaagttgctt ctgttaaaat ttcgttttgc
     2161 tacggcttct ataataggga cttttac
//
LOCUS       DAZGAP010000371         2174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGAP010000371 DAZGAP010000000
VERSION     DAZGAP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.373"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(82..1128)
                     /gene="aroH"
                     /locus_tag="ACHLAM_005262"
     CDS             complement(82..1128)
                     /gene="aroH"
                     /locus_tag="ACHLAM_005262"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO7856618.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1285..2118)
                     /gene="ppsR"
                     /locus_tag="ACHLAM_005263"
     CDS             complement(1285..2118)
                     /gene="ppsR"
                     /locus_tag="ACHLAM_005263"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO7856619.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT          536 a          545 c          568 g          525 t
ORIGIN      
        1 gcgggccaga gcaagcagta atttgttgag aagacaacaa gttgcgcaga aatgcccatc
       61 ccgtcaggaa tgggcacgca ttcagaagcg agtatctacc gcagaggcga gtttttcgac
      121 caggcgttcg gtatcttccc agcccagaca cgggtcggta atggattgac cgtaagtgag
      181 cggctgaccg ccgacgattt tttgcgttcc ttcgcgcagg aaactttccg ccataattcc
      241 agcaatcgcc gtagatccat tgcggatttg ctgacaaata tcctcacaaa cttctaactg
      301 gcgtcggtgc tgcttctggc agttaccgtg gctgaaatcc accaccagat gttcaggtaa
      361 atcaaactcg tgcagcgtat cgcaggctgc ggcgatatca tcggcatgat aattcggttt
      421 tttgccgcca cgcataataa tgtggccata cgggttgccg ctggtctgat agatggtcat
      481 ctgaccattt ttgtctggcg agaggaacat atggctggcg cgggctgcgc ggatagcatc
      541 cacggcaatc cgcgtgttgc catcggtacc atttttaaaa cctaccggac aggagagtgc
      601 cgaagccatt tcacggtgga tctgactttc ggtagtacgt gcgccaatcg cgccccaact
      661 gattaaatca gcaataaact gaccggtcac catatcgagg aactcagtcg cggtagggac
      721 gcccagctca tttacctgta aaagtaattt gcgcgccagc tccagaccgt gatttacccg
      781 atagctgccg tttaaatctg gatcggagat tagccctttc cagccgacaa cagttcgtgg
      841 tttttcaaaa taggtgcgca ttacgatttc cagccgtgac tggtactggt tgcgcagcga
      901 ctgcagacgg gtggcgtact ccattgcagc ggtgagatcg tggatcgagc aggggccaat
      961 aatgaccaac agtcgcttat cttcaccatt cagtattttt tcaattctgc ggcgggagtc
     1021 ggtgacatgg gtggcgacgc caggcgttac gggataccgt agcgcgagtt cggcgggcgt
     1081 taccaggctc tcaatacgcg cagtacggag ttcgtcagtt ctgttcatta caagtctcag
     1141 aaatttgttg cttctacggc aggactaccg ggaagtgatg cgcatcacga taaaccaatc
     1201 gcagctgatt tcaagtgcga ggtgtgtcac acctcgccgg gtggttagca tgataacaaa
     1261 aaaacaagct aatgcactag ttctctagta cattcgacgg ctaaggccca tgatatcgag
     1321 gatcttggtg gcaatctctt ctaccgaata attggtactg ttaatccacg ggatctgatt
     1381 tttacggtac aacgcttcca cttccgcgac ttccatcctg cactgacgaa gcgaggcata
     1441 gcgactgttc tcccgacgtt cctcgcgaat cgccgccaga cgttccgggt cgatagtcag
     1501 gccgaacaat ttatgctgaa gcggtttgag cgacgcgggt agcacaagat tatccatatc
     1561 gtcggcaata aaggggtagt ttgccgcgcg gataccaaac tgcattgcca gatacagact
     1621 ggtgggggtt ttaccacagc gagaaacgcc gagcaggatc acctgagcct ggtccagatt
     1681 gcgtaacgaa atgccatcat cgtgggcgag ggtgtaatca atcgcagcaa tgcgcgcatc
     1741 atatttattg agattattag ggttaaggcc atgggtacga tgagcaatcg gcgttggatc
     1801 cagtttcatc tcttgttgta gcggggcaac cagcgcctga acgatatcct ggcaaaagcc
     1861 ctcactttgc aagatgatgg cgcgaatctc cggcaacacg atggagtaga agaccagcgg
     1921 gcgcacacct gtctggtgat aaattgcgtc aatctgatcc ttcactgccc gtgcacggct
     1981 ctcattttcg acaaacggca gcgtaatgct gctgatagtg acgggaaatt gtgacattac
     2041 tgcgtgtcct aatacctccg cagttattgc cgtaccatca gaaatataaa aaacgtggcg
     2101 atcaacagca ttatccattt tgttcttccc gtgatgcaga cataattcta tgaaagcata
     2161 aattaaaaac gcac
//
LOCUS       DAZGAP010000372         2172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGAP010000372 DAZGAP010000000
VERSION     DAZGAP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.374"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(271..1074)
                     /locus_tag="ACHLAM_005264"
     CDS             complement(271..1074)
                     /locus_tag="ACHLAM_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856620.1"
                     /translation="MSADGISDLIKPEIFLVIFPGTTTTAKRGGAGWLNNKRVVFRVW
                     LYISKASYKKMGIFQRGSAPESIPYREYHFPSYPDTHEKRKKVLRDWISFMRSRFNIN
                     QIAQFCYRMENEWAFTFDNIHVPRHFREGTDDTEWSWNYIKKKRLFSLSGLQHIQPQN
                     NTERKLLINAFFDTCLIENNPADDIDIKNMLLRKMNAAFYKRKSRARARIHSRNNQQM
                     SVSSGTDKQVNIRLKDFQYQQLLFIAQKENKSKSEIIKELITRAYRAIQ"
BASE COUNT          619 a          376 c          485 g          692 t
ORIGIN      
        1 gtcgttaagt gaagaactgg cccatgtgtt attttttaca ttgttgcatt cggatataat
       61 gtccaggttt agtttaagag aattattctt cagaatagaa agtggacgtg ataataataa
      121 gaaaagggat cttatacgcg aatatgtaag cgatgtaaca aaagaatatt cgttatgtat
      181 acctagcata gtcagtcggc acgataaaaa atagtcagta tcatcccgtg catatgatgt
      241 atggtcggga tgaaatggtg aacgtggtgg ttattgtatc gcccggtatg ccctggtaat
      301 gagttccttt atgatttctg acttactttt gttttctttc tgtgcgataa aaagaagttg
      361 ctgatactga aaatccttaa gacgaatatt tacctgttta tctgttcctg aactgactga
      421 catctgttgg ttattccggg agtgaattcg cgcacgcgcc cggctctttc ttttataaaa
      481 cgctgcattc atctttctga gtaacatgtt ctttatgtca atatcgtcag cagggttgtt
      541 ttcaataagg caggtatcaa aaaaagcatt aatgagaagc tttcgttcag tattgttctg
      601 aggctgaatg tgttgcaggc cgctcaggga aaataatcgt tttttcttaa tgtaattcca
      661 tgaccattct gtgtcatcag ttccctcccg aaagtgtcgt ggaacgtgga tattatcaaa
      721 ggtgaatgcc cattcatttt ccatacgata acagaactga gctatctgat ttatattaaa
      781 acgggagcgc atgaaggata tccagtcacg taataccttt ttcctttttt catgggtgtc
      841 agggtatgag ggaaaatggt attcacgata gggtattgat tcgggcgcag aaccgcgctg
      901 aaaaatcccc atcttcttat aactggcttt actgatataa agccataccc ggaacacgac
      961 tcttttatta ttcaaccacc cggcgccacc ccgttttgct gtggttgttg taccaggaaa
     1021 aatgacaaga aatatttctg gttttattaa atcggaaatg ccatctgcgc tcatgtttgt
     1081 acctccatta aattattaaa tactgacatt ttcatgatat gtcagtcaat tagttttgtg
     1141 acatgcccat gtcgtctcag aagaacaatg acgagtctgt attatgggat attaaataca
     1201 tgctctaagg ttgtaacata taaggtcata gaagtggtct taatggtata ttattgttac
     1261 atatacttaa tgttgtggat tgtgttctgc cctgctagta tgtccggagg acgttgctgt
     1321 tctgtgagtg tcagagagtg ggaagactgg caggaagacg gtattgagca ggataaaagt
     1381 atgcgatgtc tgagcaaaga aaagcgtgta cgatgggcgt cattatcagg atgtcgggta
     1441 accacggaag gttattcaaa aaggaaatga tggattacag atgcgcaata tcggcgcggt
     1501 tttttggcgc gattttgtta atttttatat atcagcacca tctgtgacgt tgtcacatgg
     1561 tgataatttc ctgatgtgtg tcctttataa ctgacaatat ttcaatgatg tatgagaata
     1621 aacggtaata tgcaggtgcc agttcgttac tggaagatat gtttacgatg gtcatctctt
     1681 atcgttattg acggaaatct gcctgatgtt catctctttt atctgacgat cagatttaca
     1741 gggaggggat tcgcctgaaa gatatcaggt aatgcagcta ccatgataag aaaaggtatc
     1801 acttcctttc gggaagaata aatgtcccgg tcttctgcat gtgaatgttt gtcagacaga
     1861 aatgtcgtct gaatcagttt tttatcgctg cctgttcgtt cggtaaagaa caggattatc
     1921 gcttgtccga ctcacctgaa tgaatatgcc agaatgatgt tatacagccg ttgtgttgta
     1981 gaaagttttc aggtgaaaaa tatcccgaca gggaaaataa ttccagagta aatctgctcc
     2041 gttttcagaa caaaaatccc catgctggcc gtgaaaaact gaaaccaaca ttcagtctgc
     2101 atggatgttt ggaagagttg agaatgtcgg tatgatacta tatacacgta ctttcataga
     2161 caatcataca ag
//
LOCUS       DAZGAP010000373         2152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGAP010000373 DAZGAP010000000
VERSION     DAZGAP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.375"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..1196
                     /locus_tag="ACHLAM_005265"
                     /pseudo
     CDS             58..1196
                     /locus_tag="ACHLAM_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            1132..>2152
                     /locus_tag="ACHLAM_005266"
     CDS             1132..>2152
                     /locus_tag="ACHLAM_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7856621.1"
                     /translation="MRITASNLVQAISSLPKNTLFNYINDRNSGKIEIVRIQHPEGPI
                     EIKRFDPKKGQTQATAKTESISTQMLWRLANALEENRPVNVERVFGASYNTRSVLESL
                     LAHTPEFYWCRPARLEIMNAQKSIKPGHKHLIFLPDMPHTNGLLIEHQTNIVVSEMSF
                     DVVHQSVDIETIKPTKGMTIEEKRRHAQIQIALVKIGYCLGLDTWVAANDRSLQYNGK
                     AIAQMDGVISSLSDEQVLQSYNDAIKDARLIDCIWFREGRMMPAVMEIEHSTGIKSGL
                     TRMKQFYDHAPRLQDIRWTIVAPDEYRAKVIEFANMPQFIELKAKFFPYSSVEELYSL
                     CDRRKP"
BASE COUNT          717 a          418 c          439 g          578 t
ORIGIN      
        1 gtaggatcgt acagcgagat atttagtaaa aatttaagct aacttgatag gtataaaatg
       61 tccacaatga aacccgctat attttcattc ttttcaggat caggttttct tgacctaggc
      121 ttcgaaaatg ccgggtatga ggtggttttc gtcaatgaat tccatcctcc cttcatggac
      181 gcgtataaac actcaagaaa aaaacttggc aaacagccgc cacgctttgg gtatgcccaa
      241 gaaagcattg aaactattga gcattcagtg attaagcata gcatgaaaac actgaaaggt
      301 gaaaaccgcc tagtgggctt cattggcgga ccaccatgcc ctgacttttc tgttgctggc
      361 aaaaacaaag gcaaagatgg tgaaaatggc aaattatcac aaagttacat tgacctgata
      421 attgaaatga tgcctgattt cttccttttt gaaaacgtta aaggcttata ccggactctt
      481 aaacatagag aatttttcga acaaattaag aatgaactag cttcagtaga tatatcctgc
      541 atgaaagatt gattaattca attgaatacg gtgcgcctca agatcgagaa aggattattt
      601 tagttggttt tcttgataag tctttagcca aaaacttcga atggagtggc caaatatacc
      661 ccaataagga agctttccag ttcccttggc caacaacaaa cacatacgaa gaagactcaa
      721 gtatggaatg cccgcaaggt attccagaag aacttactat agaatactgg ttcgagaaaa
      781 acgatgttac caatcatcca aacgctaaac atgtatttac ggcccgagcc ggtttagcaa
      841 gattccagtc agtagaagaa ggcgacgata gtaaaaaatc atataaaagg cttcatcgct
      901 ggagatactc tccaacagca gcatatggta ataatgaagt tcaccttcac ccatacaaac
      961 ctcgtcgtat tacggtcgct gaagcattgg caatccagtc tttgccaaaa aattttgagc
     1021 taccagggga tatgactttg tcgaatatgt ttaaaacagt cggaaacgga gttccctata
     1081 tcgccgcgct cggtattgcc caatcaattt tgcaacattt aggatacagt aatgaggata
     1141 acagcatcga atctggttca ggcgatttcg agcttaccca aaaatacgct gtttaactat
     1201 ataaacgata ggaatagcgg gaagattgag attgtacgta tccaacatcc cgaaggtcct
     1261 atcgaaataa agagatttga tcctaagaaa ggtcaaactc aagcaacagc taaaacagag
     1321 agtatttcga ctcaaatgct ttggcgactt gcaaatgcac tcgaagaaaa cagacctgtc
     1381 aacgttgaaa gggttttcgg tgccagttac aacacccgct cggtattgga gagcttgctc
     1441 gcacatactc cagagttcta ttggtgtagg ccagccagac tggaaatcat gaatgcacaa
     1501 aagtctataa aaccaggaca taagcatctg atttttctgc cagatatgcc acatactaat
     1561 ggtttgttaa ttgagcacca gactaatatt gttgtctctg aaatgagctt cgatgttgtt
     1621 caccaaagcg ttgatattga gacaattaaa ccgactaaag gcatgactat tgaagaaaaa
     1681 agacgtcatg ctcagataca aattgcctta gtaaaaatag gttattgttt aggtttagat
     1741 acttgggtag cagccaatga tcgtagctta caatataatg gtaaagctat agcgcaaatg
     1801 gacggtgtaa taagttcatt atctgatgag caagtactcc aatcgtataa tgatgcgatt
     1861 aaagatgctc gtcttataga ctgcatttgg tttagggaag gccgcatgat gccagcagta
     1921 atggagattg aacacagtac tggtataaaa agtggtctga ctcgcatgaa acaattctat
     1981 gatcatgcac cgagattaca agatatccgt tggactatcg ttgctcctga tgaatatcga
     2041 gcaaaggtga tagaatttgc aaacatgccc caattcattg aattaaaagc taaattcttc
     2101 ccttactctt cagttgaaga attatactct ctatgcgacc gaagaaaacc ta
//
LOCUS       DAZGAP010000374         2149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGAP010000374 DAZGAP010000000
VERSION     DAZGAP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.376"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(94..2034)
                     /gene="iolD"
                     /locus_tag="ACHLAM_005267"
     CDS             complement(94..2034)
                     /gene="iolD"
                     /locus_tag="ACHLAM_005267"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFO7856622.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT          575 a          612 c          440 g          522 t
ORIGIN      
        1 tttattttgt agtttcaacg aacaaatact tttaccgtaa aaaacgtcag gttgcagaaa
       61 ggcgccctcc tgcaacctta aaaactgaat taattagtac tgtcgcgctt gtccaatatg
      121 ctcatcaagc atacgagcta ctgcctggat gcgttcgcta cgcgacacgc gagcgccacc
      181 aacacgccac cagctcagat atttatgcgc cattgtttta ggcagtactt taatgtctat
      241 caacgtactc accgtctgtt tacgtgcatc ctccagagct tcaagcagtt gtccaatgct
      301 ggtgacgcga tagcttttac agccatatcc ttcggcaatc cgggcaaaat ccacaggaat
      361 gagtcccccc tcctgacgac cgttatcctg tttatggaaa cgaaactcgg taaagaaact
      421 gtccataccg tgttccattt gaagattatt tatacaacca ttagtcatgt tatccagcaa
      481 aataacatta attttcttcc ccatctggac cgaagttacc agctcagaat gcagcatcat
      541 aaatgagccg tcgccaacga agctatatac ctcccgctcc ggttgcgcca acttcgcccc
      601 caatgcggca ttgacctcat agcccataca ggaatagcca tattctacgt ggtaatcatt
      661 atcgccacgg ctttgccaga tgcgctgcag atcgcctggc agacttcccg ccgcagcaac
      721 caccagcgcg ttttctggca gcattcgatt tagtactcca agaacgcgcg tctgtgtcag
      781 ccatgaatga gtaatttcat tgaactcttc aaacactttt tgagtatcca tatgatccac
      841 aatttccggt ttgaaatcct taccgctgta ttccgctgca tagacgcgat ccacttccgc
      901 cagatattga gaacgaacac gatggatcgt tttcccccat tgtgatatgt aaccttccgg
      961 agccagtcgt tcgcaaagct gcgtcagggc aacacgggca tcagcctgca attgcactcc
     1021 atccagtttg aagacgtcaa aacggctgac gtttatattc agatagcgca catcaggatt
     1081 ctggaaaatc catttagaag aggttgtgaa atcggtataa cgcgtaccca caccaataat
     1141 cagatcggcc tcctgagcca gcaaattcgc cgccagcgat ccggtttcac caatcccacc
     1201 aacattcagc ggatgcgcag aactgagcgc acctttacca gcctgcgttt cagcaatcgg
     1261 cagatgatat tgttcggcaa atcgcagcag ttcctgttca gactgcgaat acttcacacc
     1321 cccaccgcaa ataataattg gtctctggtg gcgaagaata agagcaactg catcattcag
     1381 cattagctct gttggcacgt ggcgctcaag acgatagacc cgacgtgcaa aaaaatagtc
     1441 tggataatcc caggcttctc cctgaacatc ctgcggcaaa caaagcgtca cagctcccgt
     1501 atcagccgga tcggtaagca cccgcatcgc gctaatgcag gcgctcatta gctgttccgg
     1561 acggataatg cgatcccaat agcgactcac tgcacgaaaa gcatcattgg tgctcagcgt
     1621 caggtcatgc tcctgttcaa tctgctgcaa cacaggatcg ggttgacgcg atgcatacac
     1681 atctcctggt agcagaagca acgggatacg gttcgccgtc gcagtcgccg ccgccgtgat
     1741 catattagcg gcaccaggcc ctacggagga ggtgcaggca taaatctgct gacgacgcat
     1801 ctgacgcgca aatcctgttg ctatatgtgc cataccctgt tcattgcgtc cttgatgaac
     1861 gctaagttga ccacactctt cttccagagc ctgccctaat cccacgacat tcccatggcc
     1921 aaaaattgcg aaaatacctt ttacaaactt gatttcgcga ccgtccactt caatgtactg
     1981 attatcaaga aagcgcacca aagcctgcgc catagtcaac ctgattgttt tcatgcactc
     2041 cacctatctt atcaaccgtt tgactgtcgt tacctacgta cgcactgcta cagccccggg
     2101 cataaaacac tcaccacaag aaaaaaattc cgcaaacgca attatatga
//
LOCUS       DAZGAP010000375         2109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGAP010000375 DAZGAP010000000
VERSION     DAZGAP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.377"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..449)
                     /locus_tag="ACHLAM_005268"
     CDS             complement(<1..449)
                     /locus_tag="ACHLAM_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7856623.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVL"
     gene            complement(449..1138)
                     /gene="tssL"
                     /locus_tag="ACHLAM_005269"
     CDS             complement(449..1138)
                     /gene="tssL"
                     /locus_tag="ACHLAM_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO7856624.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVILTGVIWWGGYLWLQS
                     LLSSQIPELHG"
     gene            complement(1135..>2109)
                     /gene="tssK"
                     /locus_tag="ACHLAM_005270"
     CDS             complement(1135..>2109)
                     /gene="tssK"
                     /locus_tag="ACHLAM_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7856625.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLIPVSRVPAAL
                     PVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
BASE COUNT          539 a          629 c          537 g          404 t
ORIGIN      
        1 agaaccgctt catcatgatg acagtctggc aggcagcggt acatcacaga gagctgaccg
       61 acctggcgcg ggaactgcgt ctgtatgctg cggacaacgt cagttaagcg actgacgtca
      121 ccgacccgca gccaccatcc ccgggctgtt ttacgcagcg gaccgtcctg aaacaacgct
      181 tccggcatgt ctccacatac cagtacaaca gggccccggg tgtcggataa tgccggttca
      241 gcatccgtca gggtaacgtc agatgtgagc ttttgctttt cactccggct gtttttaaaa
      301 acactccagc ctgccagaag aaggatcagc agagtagcca gaccgttaaa aacgttgcct
      361 gccgggatga aataccacag caaccacagc aacgtggaaa cggccaccag ggcatgcagg
      421 gctaccggaa tgatgacaga tccacgcatc agccatgcaa ctccggtatt tgtgatgaca
      481 aaagagactg aagccagagg taaccccccc accagataac gccggtaaga atgacggcga
      541 gcactgtcca gaaccagact gagcgtatca gacgccagcg atgtttctcc atccggtaaa
      601 caaccaggga cagaccacca tcaggcagag aaacacgttc agaaagggct ttcatgacct
      661 catcacgctg tgactggctg actgctttca ggccgtacac cccctgaaaa cccagtgctg
      721 tcacccggtg aaagcaggtt aaaacggccg ggtccggtga aggctgacgc agaacctctg
      781 caatgcgatc ccacagtgct tcaccagccc gaagagaacc aaaaaagcgg gcctgaagcg
      841 gcactttacg ccaggccgcc tggccttcgt caacgtccgg ctccccgcct tccccgcttt
      901 tttcaggctt acggctcatc accgtttcgt ccaggagggc acattgtgca taggtaatat
      961 gctccacact ggcatcgtca taaccggcac gctccagcgc ttcccgcaca ctttcgacct
     1021 gtttaacgca tttgttgtaa agcgcagtgc catcgggcgt gagtgcacct tttttcagca
     1081 tggtgacggt aagccatgtc tccatcatca gctggtcgat atcaatatcc tgtctcatga
     1141 gcgcaatacc gcaaacagtt caagttccag ggcaccaagt aactcaggca catagaacat
     1201 acacactccc tgctccagca tttcatgtgc agcgggactt tccatatcaa gtgcgaagta
     1261 ctggttttcc atacgtacag gcaacgcagc cggaaccctg ctcaccggga tcaaagggat
     1321 ccccctgagt gccgcgccat agatctcact gacattatca ggtgaacccg ccagacagag
     1381 tgccggaaat ttctcagcaa cctgccaggc cggagtatcc gagcgcacag acagccagag
     1441 gtccgcctct tcgcgcaggc ggatatcgtg aaggctggcc ttccaggtct gtccatccgg
     1501 gcgggacatt tccagagcaa tgacccggga tggcagacag gcctccagca gacccgttat
     1561 caggtcgaac agcggaagaa acgcaccggc cggatccttg tggtcataac ccggtatggc
     1621 gtcaggatca tgatccaggg aaaaagtcag catactgcct gccagacgag cgagctccgc
     1681 ccacacctgt tcaggtggac gggaaggaaa acgttcgtat tccgtcagta cactggcgtg
     1741 ggagttcagc gcattcagca accagaaaag ggaaacatca gcgacagcaa aatcggcgag
     1801 tctttcgtta ctttcacgac gcatcgccat cagacgctgg cgtcgggagc gtaactgacg
     1861 attgagcaac accagacgtt cgcgcagaac cggacttgcc ccaaataacg ccaccggagg
     1921 aataaaagac ggatcctgtc gccacgcatt ctgaccatct ctgatcaatc ttgctaccgg
     1981 acagactttc catgaatcat tgttttcatg ggcaaaccgg atggtgaaat taaaccgggc
     2041 aacggctatg ggctcttctt cagagccaaa agcatcctgc agcgtgaccc actcttcccg
     2101 atagcgaac
//
LOCUS       DAZGAP010000376         2085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGAP010000376 DAZGAP010000000
VERSION     DAZGAP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.378"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            35..568
                     /locus_tag="ACHLAM_005271"
     CDS             35..568
                     /locus_tag="ACHLAM_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7856626.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            842..1537
                     /locus_tag="ACHLAM_005272"
     CDS             842..1537
                     /locus_tag="ACHLAM_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO7856627.1"
                     /translation="MKEMISVDREISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPQTLPEALRFAAKLAEQKAELENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1632..1763
                     /locus_tag="ACHLAM_005273"
     CDS             1632..1763
                     /locus_tag="ACHLAM_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856628.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            1775..2020
                     /locus_tag="ACHLAM_005274"
                     /pseudo
     CDS             1775..2020
                     /locus_tag="ACHLAM_005274"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            1986..>2085
                     /locus_tag="ACHLAM_005275"
     CDS             1986..>2085
                     /locus_tag="ACHLAM_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856629.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPATVPCVKP"
BASE COUNT          542 a          453 c          583 g          507 t
ORIGIN      
        1 ggtattcgga accgataaat cctgataaat atccatgaac gcaaaaatca gatacggcct
       61 gtcggctgcc gttctggcgc tgattgccgc aggggcgcct gcgcctgata ttctcgacca
      121 gtttctggat gaaaaagaag gtaaccacac cacggcatac cgtgatggtg cgggtatctg
      181 gaccatttgt cgtggtgcca ccagggtgga tggcaaacct gtcgtcccgg gcatgaagtt
      241 gtcgaagaaa aaatgcgacc aggttaacgc cattgagcgt gataaggcgc tggcatgggt
      301 ggagaaaaac atccgggtgc cactgaccga accccagaaa gcggggatcg cgtcattctg
      361 cccgtataac attggtcccg gtaagtgttt cccgtcgacg ttttataaac gaattaatgc
      421 aggcgatcgc aggggagcgt gtgaggcgat tcgctggtgg attaaggacg gtggcagaga
      481 ctgccgtatt cgctcaaata attgctacgg tcagatatcc cgtcgtgacc aggagagtgc
      541 gctggcgtgc tggggaatcg acagataagc agaatatttt gctgaaaaat gacgttggtc
      601 aacgcgggtg gataacacga aatcctgcga actggcaaaa cctaagtgaa taaagtcagg
      661 aacattgttt cacgcagagg caccgtaatg gtgcctttgt catttctgcg cttcgcacaa
      721 gcgtaaataa accaaagaac ctttcaggat gagccctggt ggataaccgg cagtggtctg
      781 gttaaccctc tttgggctgg ttattcctgt gcgcagggtt catcactaaa aggaatcaac
      841 catgaaagag atgatttctg tcgatcgtga aatatccatg agtagtctgg attttctgaa
      901 taacattatt aatccagccc gggcagaagc cggagaagtc cctcatgaac cgcgtaagtt
      961 tcttgcaaaa attgaggatg agctagagct tgatggaacc ggaaaaaaat tccggttaaa
     1021 caataaccag acaagaacgg catactatga tctggatttt gaccagatga tgctcgttgg
     1081 catgagagag tcaaaggccg ttcgtcgttc tgtgctggca aggctcaaag cgatgcatgg
     1141 tattcagatc ccccagactt tacctgaggc gttgcgattt gcggcaaaac tggcagaaca
     1201 gaaagcagag ctggaaaatc aactggcaat agcagcaccg aaggctgaat ttgttgataa
     1261 ctatgttgaa gcatctggtc tgatgggatt ccgggaagtt gctaagttac tcggtatcaa
     1321 agaaaccgat ttccggctgt ttttgttgga gaacggaata atgtatcgcc tcgctggaaa
     1381 aatgacgcct tactcgcatc acctggatgc ggggcggttt agcgtgaaaa cgggcgaggc
     1441 gggcaatggt catgctttta cgcaggttaa attcacccca aaaggtgttc agtggattgc
     1501 tggtctgctt gctgcatgga gagctaccgc agcatgaaga tgataaaaat ggactggaaa
     1561 tttttgctgg tctggctgat tccgttttta tgggtggttg cctggttaat tactgctatt
     1621 aagaggtaaa gatgtcagac aaactcataa tgctggcgaa gggcctttgt gtaatcgtcg
     1681 gtatttcatt ttcactaatg ctggttgctc tttttctttc cataacctgg atggcgttga
     1741 cttcggcagg gctggtgggg tgagcataaa ccgaatgctt tccgcgttta ccgttattct
     1801 gctggtggtc tgtggtgcgc ttagtctggg gctgaatcat taccgcgata acgccatcac
     1861 ctacaaagcg cagcgcgata aaaaagccag agagctggag ctggcaaacg caaccattac
     1921 tgacatgcag gtgcgccagc gcgatgttgc tgcgctcgat gcaaaatact cgagggaatt
     1981 agccgatgcg agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg
     2041 cctgcggatc aacgccacct gccacggtac cgtgcgtgaa gccac
//
LOCUS       DAZGAP010000377         2084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGAP010000377 DAZGAP010000000
VERSION     DAZGAP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.379"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2084
                     /locus_tag="ACHLAM_005276"
     CDS             <1..>2084
                     /locus_tag="ACHLAM_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7856630.1"
                     /translation="SLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQ
                     YCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRP
                     SDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDG
                     ILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIE
                     ICDNDYAGTLTGGRILSIDAASRTLTLDREVILLEAGTSMVNLINGSGKPVRVDITAH
                     PAPDRIQVSALPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKE
                     AIVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLR
                     LTSGNGENSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAP
                     AAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGRQW
                     SVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQG
                     MWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLA
                     ALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVA
                     QGNQLFMNDVFL"
BASE COUNT          465 a          568 c          668 g          383 t
ORIGIN      
        1 agtctgaaac cggcatacag caataacccg gcctggtgcc tgtgggacat gctgacccac
       61 ccgcgctacg gcatgggaaa acgcctgggg gccgcggatg tggacaagtg ggcactgtat
      121 gccatcgggc agtactgtga ccagacggtc ccggatggtt tcgggggcac agagccgcgg
      181 atgactttta atgcgtacct gtcacagcag cgtaaggtct gggatgtcct gggggatttc
      241 tgctcggcga tgcgctgtat gccggtatgg aacggccaga cgctgacgtt cgttcaggac
      301 cgcccgtcgg atgtggtgtg gccctacacc aacagcgatg tggtggtgga tgataacggc
      361 gtggggttcc gctacagctt cagtgccctg aaggaccggc acacggcggt ggaggtgaat
      421 tacaccgacc cgcagaacgg ctggcagact tccacggaac tggtggaaga cccggacggc
      481 atcctgcgct acgggcgcaa cctgctgaag atggatgcgt tcggctgtac cagccgcggt
      541 caggcccacc gtgccggact gtgggtgata aagaccgaac tgctggaaac gcagacggtg
      601 gatttcacgc tcgggtcaca ggggctgcgt cacacacccg gtgacatcat tgaaatctgt
      661 gataacgact atgccgggac cctgaccggc ggacgcatcc tgtccatcga tgccgccagc
      721 cgtaccctga cgctggaccg tgaggtgata ctgctggaag cagggacatc gatggtgaac
      781 ctgattaacg gcagcggtaa gccggtgcgc gtggacatca ctgcacaccc cgcccctgac
      841 cggatacagg tcagcgccct gccggatggc gtggagacat acggtgtgtg gggactctcc
      901 ctgccgtcac tgcgtcgtcg cctgttccgc tgtgtttcca tccgggaaaa cacggacggt
      961 acctttgcca tcacggcggt gcagcatgtg ccggaaaaag aagccattgt ggataacggg
     1021 gcccgctttg agccgatgtc cggctcactg aacagcgtca tcccgccggc ggtgcagcac
     1081 cttacggtgg aggtgagtgc ctcagacggc cagtatctgg cactggcgaa atgggacacg
     1141 ccgcgggtgg tgaagggcgt gcgcttcagt ctgcgcctga ccagcggaaa cggtgaaaac
     1201 agccgcctgg tgaccaccgc catcactgcc gacacggagc accgtttcag tggcctgcct
     1261 ctgggggaat acaccctgac ggtcagggcg ataaacagct acggccagca gggcgaacct
     1321 gccaccacca cgttccggat taacgcaccg gcagcaccgg ccagcattga actgacgccg
     1381 gggtattatc agataacggc ggtcccgcat cttacggtgt atgacccgac ggtacaattt
     1441 gagttctggt tttcggaggc aaaaatcgca gacacatctc aggtggaaac ctctgcccgt
     1501 tatctgggga caggccgcca gtggagtgtc tccggcccgc acattaagcc ggggaaggat
     1561 ttctggtttt atgtgcgcag cgtcaacctg gtggggaaat ctgcgtttgt ggaagccagt
     1621 ggccgggcca gcaatgatgc ggaagggtat ctggactttt tcagaggaga aatcgggaag
     1681 acacatctgg cacaggggat gtgggagctg attgataaca gccagcttga tgatgagatg
     1741 gcggagatga agaccaccat cacagaaacc cgcaatgaaa tcacgcagac ggtcagtaaa
     1801 accctggaag accagagtgc caccatacag cagatacagc gggtgcagac agacacaaat
     1861 aacgacctgg ctgcgctgta catgctgaag gtgcagaaaa cgaaaaacgg cattccgtat
     1921 gttgccggta taggtgcggg gattgaggat gctgatggcc agcccctgag caatatactg
     1981 ctgcaggcgg accgtatcgc gatgattaac ccggagaacg gcaacaccac gccgctgttt
     2041 gtggcgcagg ggaatcagct gttcatgaac gatgtgttcc tgaa
//
LOCUS       DAZGAP010000378         2084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGAP010000378 DAZGAP010000000
VERSION     DAZGAP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.380"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2084
                     /locus_tag="ACHLAM_005277"
     CDS             <1..>2084
                     /locus_tag="ACHLAM_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7856631.1"
                     /translation="SLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAPDVDKWALYAIGQ
                     YCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRP
                     SDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDA
                     ILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIE
                     ICDNDYAGTLTGGRILSIDAASRTLTLDREVILPEAGTSTVNLINGSGKPVRVDITAH
                     PAPDRIQVSVLPDGMETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKE
                     AIVDNGARFEPLSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLR
                     LTSGSGENSRLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTAFRINAP
                     AAPASIELTPGYYQITAVPVLAVYDPTVQFEFWFSEKQITDILQTETSARYLGTGSQW
                     SVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQG
                     MWELIDNSQLDDEMAEMKTIITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLA
                     ALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVA
                     QGNQLFMNDVFL"
BASE COUNT          464 a          566 c          674 g          380 t
ORIGIN      
        1 agtctgaaac cggcatacag caataacccg gcctggtgcc tgtgggacat gctgacccac
       61 ccgcgctacg ggatgggaaa acgcctgggg gccccggacg tggacaagtg ggcactgtat
      121 gccatcgggc agtactgtga ccagacggtc ccggatggtt tcgggggaac agagccgcgg
      181 atgaccttca atgcgtacct gtcacagcag cgtaaggtct gggatgttct gggggatttc
      241 tgctcggcga tgcgctgtat gccggtatgg aacggccaga cgctgacgtt cgttcaggac
      301 cgtccgtcgg atgtggtgtg gccgtacacc aacagcgatg tggtggtgga tgataacggc
      361 gtggggttcc gctacagctt cagtgccctg aaggaccggc acacggcggt ggaggtgaat
      421 tacaccgacc cgcagaacgg ctggcagact tccacggaac tggtggaaga cccggacgcc
      481 atcctgcgct acgggcgcaa tctgctgaag atggatgcgt tcggctgtac cagccgcggt
      541 caggcccacc gtgccggact gtgggtgata aagacagaac tgctggaaac gcagacggtg
      601 gatttcacgc tcgggtcaca ggggctgcgg cacacgcccg gtgacatcat tgaaatctgt
      661 gataacgact atgccgggac cctgaccggc ggacgcatcc tgtccatcga tgccgccagc
      721 cgtaccctga cgctggaccg tgaggtgata ctgccggaag cagggacatc gacggtgaac
      781 ctgattaacg gcagcggtaa gccggtgcgc gtggacatca ctgcacaccc cgcccctgac
      841 cggatacagg tcagcgtcct gccggatggc atggagacat acggtgtgtg gggactctcc
      901 ctgccgtcac tgcgtcgtcg cctgttccgc tgtgtttcca tccgggaaaa cacggacggc
      961 acttttgcca tcacggcagt gcagcacgta ccggaaaaag aagccatcgt ggataacggg
     1021 gcccgctttg agccgctctc cggttcactg aacagcgtca tcccgccggc agtgcagcac
     1081 ctcacggtgg aggtgagtgc ctcagacggc cagtatctgg cgctggcgaa atgggacacg
     1141 ccgcgggtgg tgaagggcgt gcgcttcagt ctgcgcctga ccagtggcag tggtgaaaac
     1201 agccgcctgg tgaccagcgc catcacggcg gacacggagc accgtttcag tggcctgccg
     1261 cccggggaat acaccctgac ggtcagggcg ataaacagct acggccagca gggcgaacct
     1321 gccaccaccg cgttccggat taatgcgcct gcagcaccgg ccagcattga gctgacgccg
     1381 gggtattatc agataacagc agtaccggtg ctggcggtgt atgacccgac ggtacagttt
     1441 gagttctggt tctcggaaaa gcagattacg gatatccttc agactgaaac gtccgcccgt
     1501 tatctgggga caggcagcca gtggagtgtc tccggcccgc acattaagcc ggggaaggat
     1561 ttctggtttt atgtgcgcag cgtcaacctg gtggggaaat ctgcgtttgt ggaagccagt
     1621 ggccgggcga gcaatgatgc ggaagggtat ctggactttt tcagaggaga aatcgggaag
     1681 acacatctgg cacaggggat gtgggagctg attgataaca gccagcttga cgatgagatg
     1741 gcggagatga agaccatcat caccgaaacc cgcaatgaaa tcacgcagac ggtcagtaaa
     1801 accctggaag accagagtgc caccatacag cagatacagc gggtgcagac agacacaaat
     1861 aacgacctgg ctgcgctgta catgctgaag gtgcagaaaa cgaaaaacgg cattccgtat
     1921 gttgccggta taggtgcggg gattgaggat gctgatggcc agcccctgag caatatactg
     1981 ctgcaggcag accgtatcgc gatgattaac ccggagaacg gcaacaccac gccgctgttt
     2041 gtggcgcagg ggaatcagct gttcatgaac gatgtgttcc tgaa
//
LOCUS       DAZGAP010000379         2031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGAP010000379 DAZGAP010000000
VERSION     DAZGAP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.381"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1115)
                     /locus_tag="ACHLAM_005278"
     CDS             complement(<1..1115)
                     /locus_tag="ACHLAM_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO7856632.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNGSLIESGGYQDVGGHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLDSKSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNT"
     gene            complement(1180..1299)
                     /locus_tag="ACHLAM_005279"
     CDS             complement(1180..1299)
                     /locus_tag="ACHLAM_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO7856633.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(1454..1720)
                     /locus_tag="ACHLAM_005280"
     CDS             complement(1454..1720)
                     /locus_tag="ACHLAM_005280"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7856634.1"
                     /translation="MLRVTTSAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
BASE COUNT          545 a          501 c          383 g          602 t
ORIGIN      
        1 atggtatttg tcgcgtttcc ggcaacatcc ataacgccgc cattatctat tcgggtcgca
       61 tcagctttag cattcgttaa aactgacatt gttcctttat ctttaataat cgtcttattt
      121 gccgaaccat atgcgtttat gtctaaatga ccaccgtttt ccagcaacac attgtctgcc
      181 acgttattgt ggatggagaa tgccccttca ctattcgtac cgctcaccgt caaatcgtaa
      241 gtcgttactt tcaaagcggc tccatcgtgc tgagtaacat ttgttgccat accacctcta
      301 acatcaagca cgccaccgga ataaatttca ataacatccg aggtgctagt gtaatcaaca
      361 atttgcgtac caccagaata gatctgagta ttttttgccg ttgacttact atccagagac
      421 tgagttccac cttcaatcgt cgtgtccagc gcacggctct catatactct ttgctcaccg
      481 ccatttttaa tggtggttgt ttctactgtg ctctgttcaa catactgtcg accaccattt
      541 atggttgtgt tcgtcgccag acttccttgc gtgatgtcct gataaccgga cttatttatc
      601 gttgtaccat caacatgccc ctgagttgtt actgtctgcc ctccaccatc aatgatggtt
      661 ccattcgcag tccccccact tatgtgtgaa ttaccaccct taattgtcgt tccattgctg
      721 ataccacctg tataaacgtc ctgattgcca ctctcgatta ttgtacctgt ggaaataccc
      781 ccgtcatgaa ttgactgtct gcccccgtta atggttgtat tattagcctg ccccacaaaa
      841 ttgttatgac ctcctacatc ttgatatcca cccgattcaa taagacttcc attagatatc
      901 ccgccataaa cattctgctg gccatggttg ataatatgag tgttatttgt tgtacctcgt
      961 tcatctactt tttggctgcc atctacagtc tcatcgttta ccacaccaat aacatcagga
     1021 gtgaaggccg ccatcccggg cggggcatat atcaaggcag atatcaataa ggaaagtact
     1081 gagcgacgac aataataggg actggtcctg ttcataaatt tcatcctctg aaaagtgaat
     1141 actgagtagc gtttaagcga ccttagcttt gctgcaacat cagcccacag gcaccagacc
     1201 aggggattca tcctgaagag acagcgcaag tgtattgtgt tcaccgctca tcaaagacat
     1261 catgatgaaa tgatgatatt ccgcataaga atgaggcatt ttttaaacgc agtgcgctga
     1321 agtgtggttg gataaaaaag tcaatctatt caggaaacac gggcgtattc ttttctttcg
     1381 acaatcaggc agtcggaaaa ataaaatgat ttacataatc gtttctgatg aatatcttct
     1441 gctcacataa aaatcacaca ataactctga gatcgcagat tgttttactt ttacagcttc
     1501 cgtcccccca ttgttgggca aatatagatt gggccagagc acgaaagtta ataccacgtt
     1561 cgcacagctc ctccaacagc acgacaagat gccacatact gcgccccagt cggttcagtt
     1621 tacagactag cagtgcgtcc cctaccgata atgtcctgag ccgtttgttc tgtccgggcc
     1681 tgctgccacg gttatgcgct gaagtggtaa ctctgagcac gcagttcttc agtcaggcgt
     1741 ggtgcaccgt caccctatta ttgctgggta agatcaaaaa actttcaagc agctaaggaa
     1801 agttgaacca gacattagat gaaatatttc aaccaattac agcaccagtt cagacaccgc
     1861 caactcatca aataaatcaa ggggttacgt gaaagcgtag ccccttttac tttggtagtg
     1921 acagcaaaat ggatgcagtg tgaagaataa tcccgtttac tcaatcaata atacatatta
     1981 tttcaatcta cgttattatt tctttgtaaa aatcgccatt tatttatcat t
//
LOCUS       DAZGAP010000380         1986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGAP010000380 DAZGAP010000000
VERSION     DAZGAP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.382"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..574)
                     /gene="argR"
                     /locus_tag="ACHLAM_005281"
     CDS             complement(104..574)
                     /gene="argR"
                     /locus_tag="ACHLAM_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO7856635.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1009..1947
                     /gene="mdh"
                     /locus_tag="ACHLAM_005282"
     CDS             1009..1947
                     /gene="mdh"
                     /locus_tag="ACHLAM_005282"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7856636.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          515 a          437 c          517 g          517 t
ORIGIN      
        1 cgcgataaaa cgcactattc gttaaattaa tcgcaggaga aagcaggggg ggtgagaggg
       61 aaaagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
      121 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtagtaa
      181 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
      241 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
      301 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
      361 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
      421 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaatg
      481 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
      541 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
      601 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
      661 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
      721 acggcatgca aattctgctt aaaagtaaat taattgttat caaattgatg ttgttttggc
      781 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
      841 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
      901 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
      961 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
     1021 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
     1081 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggctgtc
     1141 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
     1201 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gtgtagcgcg taaaccgggt
     1261 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
     1321 gttgcgaaaa cctgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
     1381 gttgcgattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
     1441 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
     1501 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
     1561 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
     1621 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
     1681 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgcgc actgcagggc
     1741 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc acgtttcttc
     1801 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
     1861 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctgaagaa agatatcgcc
     1921 ctgggcgaag agttcgttaa taagtaattg attagcggat aataaaaaac cggagcacag
     1981 actccg
//
LOCUS       DAZGAP010000381         1972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGAP010000381 DAZGAP010000000
VERSION     DAZGAP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.383"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..1489)
                     /gene="pykF"
                     /locus_tag="ACHLAM_005283"
     CDS             complement(77..1489)
                     /gene="pykF"
                     /locus_tag="ACHLAM_005283"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO7856637.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1497..1691)
                     /locus_tag="ACHLAM_005284"
     CDS             complement(1497..1691)
                     /locus_tag="ACHLAM_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856638.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          495 a          457 c          483 g          537 t
ORIGIN      
        1 gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat atacaaatta
       61 attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg ccgctcggta
      121 ccagtgcacc agaaaccata actacgacgt cacctttgtg tgccagaccg ctctgcagag
      181 ccagttcttt acccagacgg tagaaatcat cagtagaagt gatctcttta accagctgcg
      241 gcacaacgcc tttgctcagt accaactgat gagccgtttt ttcgttggtg gtcagtgcca
      301 ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg ccctgggtag
      361 caaccacgat cagcggggca tccagttttt cagcagtttc aacggcacca cggcataccg
      421 cttcggtaat gcgcagttta cggttgtcat tgttgaactc gagacggctg ttcatcacgc
      481 ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc ttccagcggg tatttacctt
      541 ttgcggattc accagacagc atcactgcgt cagtaccgtc gaggatggcg tttgcaacgt
      601 caccggcttc tgcgcgagtc gggcgtgggt ttttaatcat ggaatccagc atctgggtcg
      661 cagtgataac gactttacgt gcacggatac atttttcgat catcatcttc tgggcgaaga
      721 taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg atgccgtcag
      781 aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt ttggagatga
      841 tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata acgtcagaac
      901 gcttacgaat aaaggaagca gcaacaaagt ctacgccttg ttcgcaacca aagatcaggt
      961 cctgtttgtc tttttcagcc agtgctggca gggcaatgga aacgccaggc aggttcacac
     1021 ctttgttttc acccaggtcg ccgttgttca gcactttaca gataactttg ttaccttcaa
     1081 tggcggtaac ttccataccg atgagaccat cgtcaaccag tacggtgttg ccaacagaca
     1141 ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg ataacagatt
     1201 tatcagtggt gaaagtaaag gtctgacccg ctttcagaga aacgtcgtta ccgccttcca
     1261 gtttcatggt gcggatttcc ggacctttgg tatcaagcag gatagcggcg gttttaccag
     1321 ttttgctcat cacgttgcgc agattctgaa tgcgctgacc gtgttctgca tagtcaccat
     1381 gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct aacatctctt
     1441 cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg acagtcttag
     1501 tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg gaaagcgaat
     1561 ccggtattac gaaagtggtg gtgcctcgct ctaaggatag gtgacatcga aaaagcgtgc
     1621 agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag tcgttgagtt
     1681 ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat acgctgaaat
     1741 taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt agacaataaa
     1801 ttgttatcaa agatttaacg ttccagggaa aaggttacgc aaaaacttcg ctgtttgcgc
     1861 aacgtctggt gccggggttg tcactgtgaa gataagtgaa agttcatccg gtgtataaat
     1921 acatcatcgc tcaattgcta cagttaaaat aatttatcgc gttatttgtc tg
//
LOCUS       DAZGAP010000382         1964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGAP010000382 DAZGAP010000000
VERSION     DAZGAP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.384"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(213..866)
                     /gene="ribB"
                     /locus_tag="ACHLAM_005285"
     CDS             complement(213..866)
                     /gene="ribB"
                     /locus_tag="ACHLAM_005285"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO7856639.1"
                     /translation="MNQTLLSSFGTPFERVENALVALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(970..1118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1240..1539
                     /gene="ubiK"
                     /locus_tag="ACHLAM_005286"
     CDS             1240..1539
                     /gene="ubiK"
                     /locus_tag="ACHLAM_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO7856640.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(1574..1774)
                     /gene="glgS"
                     /locus_tag="ACHLAM_005287"
     CDS             complement(1574..1774)
                     /gene="glgS"
                     /locus_tag="ACHLAM_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO7856641.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT          564 a          493 c          425 g          482 t
ORIGIN      
        1 cacatatcct ttttcggata tattcatatg gtcgcataat aacggaacaa ttatatgatt
       61 ttgatcataa tgaaaaagaa tgcctgatat caatgaaatt ttctcataga aagagaagcg
      121 gaattaacat cttgcatagc agggcaaaat aaaaaccgaa gccagctaca acttcggttt
      181 cttgattaag acagtaaatt aagcagcgat tttcagctgg ctttgcgttc atgcgcctga
      241 cgataagcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt ggcaaactca
      301 atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca cagcacacca
      361 gccggtttaa agccagccag cgtcatcaga tcaatagtcg cttcagtatg gccgccacgc
      421 gtcagtacac caccagcctg agcgcgaagt gggaaaacgt ggcccgggcg attcagatca
      481 gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taatacggtc agcggcagaa
      541 acaccggtag tcacaccttc agctgcttca atggtcacgg taaaaccggt gccataggcg
      601 ctggtgttat tttctaccat cattggcaga tcgagttgtt tacggcgatc ttcagtgatg
      661 cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc aacggtcatg
      721 gtttctgccg ggaagatcat atcaccttcg ttttcacggt cttcatcatc aagcaccatt
      781 acaccgcgtc cttcacgcag cgcaaccagt gcattttcaa cacgttcgaa aggcgtacca
      841 aaagaggaaa gtagcgtctg attcatggta aaaaaacctc actaaaatta tggttaccag
      901 aatcagggca gtcttaggag tggcggcata tagccaaaat aacgtgagcg ggtccatgcc
      961 cgacagaatc gttactctct cccatccgga ctctaaccgt cggccccgga attacaccgg
     1021 atctgctgtc ctttgagttc gcacccaaag cgctcgcggg ctttcaactg agttgattta
     1081 ccgccggtgg ggaatttcgc cccgccctga gaataagcgg attcactata acgctaatga
     1141 ttagcggcag caacgcatag cttcacataa ttctggttta tgacttaccc ttatcgcact
     1201 acaatggcac tcaacaccta tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt
     1261 gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag gaatcaggga gttcggggaa
     1321 gatgtggaga aaaaaatccg ccaaacccta caagcgcagc tgacgcgtct cgatctggta
     1381 agccgcgaag aattcgacgt tcagacgcaa gtcctgttac ggactcgtga aaaactggcg
     1441 ttactggagc agcgcatcag tgaactggaa gcgcgcaata attcggtagc agatcttcaa
     1501 tctcctccgg ctatcccgcc tattgataaa gcagaataaa gcatcacaaa aacgggccag
     1561 acggcccgta atatcagtgt tctaattcaa gttctctggt ttgcatcatc tgttggcaat
     1621 accaggcata acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg
     1681 ccagaatatc gaccgggcga ccttctgcgt gcattctggc aaaactacgc gccaggaaat
     1741 cgaaattatt taaagaatta agactatgat ccataagcac atcctccggt tatcgcatac
     1801 cttttaaggc catatgcttt ttctgatttc agtattcccc aatcagtgta gtgcctttaa
     1861 acataacgtg cgtaaatata ttgtccccga tcacactttt tagtgaaaaa taaaaaacca
     1921 tattaaaaga ctttctcttc cagaacttct ctaacatgtg ttat
//
LOCUS       DAZGAP010000383         1925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGAP010000383 DAZGAP010000000
VERSION     DAZGAP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.385"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..421
                     /locus_tag="ACHLAM_005288"
     CDS             44..421
                     /locus_tag="ACHLAM_005288"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7856642.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELK"
     gene            421..768
                     /gene="tnpB"
                     /locus_tag="ACHLAM_005289"
     CDS             421..768
                     /gene="tnpB"
                     /locus_tag="ACHLAM_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7856643.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            818..>1925
                     /locus_tag="ACHLAM_005290"
     CDS             818..>1925
                     /locus_tag="ACHLAM_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO7856644.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKSGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRG"
BASE COUNT          437 a          539 c          562 g          387 t
ORIGIN      
        1 agtggtatcc accaaataag tagtgggaac caaagtgtca gatatgcaga aaaatgtgac
       61 tcccggcagg cgaaaaggct gccctaatta tcctcccgaa tttaaacagc agctcgttgc
      121 tgcctcctgt gagcccggga tatccatctc aaaacttgct cttgaaaatg gcattaacgc
      181 caatctgttg ttcaaatggc gacaacaatg gcgcgaggga aagctgctat taccttcttc
      241 agagagcccc cagctacttc ctgtgactct cgatgcagct gccgaacagc cagaatcgct
      301 cgcagaggac ccggaaaccc tcagtatcag ctgtgaggta acgttccggc acgggacgct
      361 ccgcttcaat ggcaatgtca gcgaaaagct cctgactctg ctgatacagg aactgaagtg
      421 atgatcccgt taccttccgg gaccaaaatt tggctggttg ccggtatcac cgatatgaga
      481 aatggcttca acggcctggc tgcgaaagta caaacggcgc tgaaagacga tcccatgtcc
      541 ggccatgttt tcattttccg gggccgcagc ggcagtcagg ttaaactgct gtggtccacc
      601 ggtgacggac tgtgcctcct gaccaaacgg ctggagcgtg ggcgcttcgc ctggccgtca
      661 gcccgtgatg gcaaagtgtt ccttacgcag gcgcagctgg cgatgctgct ggaaggtatc
      721 gactggcgac agcctaagcg gctgctgacc tccctgacca tgctgtaaat ctctttatcc
      781 tggttgtcac agaataagcc cggtaaaata cgggcttatg aacgacatct cttctgacga
      841 catcttcctg ctgaaacagc gcctggccga acaggaagcg ctgatccacg ccctgcagga
      901 aaagctgagc aaccgggagc gcgaaataga ccatctgcag gcgcagctgg ataaactccg
      961 ccggatgaac ttcggcagtc gttccgaaaa agtctcccgc cgtatcgcac aaatggaagc
     1021 cgatctgaac cggcttcaga aagagagcga tacgctgact ggtagggtgt atgacccggc
     1081 agtacagcgt ccgttgcgtc agacccgcac ccgtaagccg ttccctgaat cactaccccg
     1141 tgacgaaaag cgactgttgc ctgcggcgcc gtgctgcccg aactgcggcg gttcactgag
     1201 ctatctgggc gaggataccg ccgaacagct ggagttgatg cgtagcgcct tccgggttat
     1261 ccggacggta cgggaaaaac atgcctgtac tcagtgcgat gccatcgtgc aggcacctgc
     1321 accttcgcgg cccatcgagc ggggtatcgc cggaccgggg ctgctggccc gcgtgctgac
     1381 ctcgaagtat gcagagcaca ccccgctgta tcgccagtca gaaatatacg gccggcaagg
     1441 tgtggagctg aggcgttcac tgctgtcggg ctgggtggat gcatgctgcc ggctgctgtc
     1501 tccgctggaa gaggcgcttc atggctatgt catgactgac ggcaaactcc atgccgatga
     1561 taccccggtc caggtactgc tgccgggtaa taagaagacg aagagcgggc ggttgtgggc
     1621 gtatgttcgt gatgaccgca atgccgggtc agcgttggca cctgcagtgt ggttcgctta
     1681 cagcccggac agaaaaggca tccatccgca gactcatctt gcctgcttca gcggtgtgct
     1741 gcaagcggat gcgtacgccg ggttcaacga gctgtatcgc aatggtggga taacggaagc
     1801 tgcctgctgg gctcatgccc gccgaaagat ccacgatgtg cacgtccgca tcccgtcagc
     1861 actgacggaa gaagccctgg agcagatcgg tcagttgtac gccatagagg cggatataag
     1921 gggaa
//
LOCUS       DAZGAP010000384         1880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGAP010000384 DAZGAP010000000
VERSION     DAZGAP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.386"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..295)
                     /locus_tag="ACHLAM_005291"
     CDS             complement(<1..295)
                     /locus_tag="ACHLAM_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-cystathionase"
                     /protein_id="HFO7856645.1"
                     /translation="MDIFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYHQQYQTDIKPEWITYSPSVMY"
     gene            complement(295..1875)
                     /locus_tag="ACHLAM_005292"
     CDS             complement(295..1875)
                     /locus_tag="ACHLAM_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO7856646.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQNIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADTPTLDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGMR"
BASE COUNT          582 a          448 c          368 g          482 t
ORIGIN      
        1 agtacatcac actggggcta taggttatcc attcaggttt aatgtctgtt tgatattgtt
       61 gatgatacca gtttactatc gcattcagat attcaccatg ttgccaacga ctgtacccta
      121 aaatcgggtg ttccaggcgt ttttttaatg ctctggtgat agcgtctggt atcggaagat
      181 ccatatctga aatagagaag ggtaatacat cttttacacc aaatctgtcc tcgcaaaagt
      241 cccattgggt gcaatatgtt cctttacggg aaataggagt attaaaaata tccatcagcg
      301 catccccatg tagttatcca tatccctacg cagggcgatg actttagtgc cgataatgac
      361 ttgtagggta ttttgatcaa gtttaacaac cgcaatcccg ccagctcttt ttattgcaac
      421 ttcatcaatc aatcccatat ctgcgattgt taaacgtaaa cgagttacgc aattatccag
      481 tgacttaata ttctctttac caccaagtgc cttaaccatc tgctccgctg cttgcaactc
      541 tgatttatca agagttgggg tatccgctat agccgtatct tcgtgagcgt tcagcgtgac
      601 aggactggac tcttcacgtc ctacggtcat caggttaaat ttgagaatgg tccaacggaa
      661 cacaaagtaa taaatgaaga aataggccac ttcaacacac accgcgatcg gccaacctgt
      721 tcttgtgcca cgcaatacgc caaagctaat aaattgaatc agatcgccag taaatcccat
      781 cttcacgccc atatatggca agaccatgtt ggcgaatcca ttcataaagg catggaagac
      841 aaacagcagc ggactgacaa ataagaacag aaattcaatc ggttcgctga cgccaccaat
      901 aataaccgta agcacaccgg aaataagcaa tgctttgata gcctttctgt tttgagcatg
      961 agcagtgcga tacatggcca gtgcagcacc agccagaccg tactgaatca tcagtttgcc
     1021 ctgctccatt ttccccgcca aatccagcgg aatgaccgca ttctctttga cgtacgccat
     1081 aaacatgttc agggtgccgt aatactcttc gccgccaatc acagccgaac cgccgattgg
     1141 cgtaaagcgg aacactgacg tcaccagatg gtttaagcca aaaggaatcg tcacacgttc
     1201 ggcaactgca tagaagaaat aaccaacagg accggaagtt gatatccagt ggccaatgag
     1261 catgaagaga ttgaaaaatg gcggccagat aaaaggaatg atcagaccaa atccactcat
     1321 cacaatcagt gtaatgatag gcaccagacg tgggccgcta taaaaaccta atgattctgg
     1381 aatgcgtaaa ttaacgatct ttttatacat gctggcgact aataacccag caacaattcc
     1441 ccctaacacg ctggtattgt aggactggat ccccagaatg atgttttgcc catgtgtcga
     1501 catttggtca gcaacgacca ataagtcgtg ctgtttaaga taaaagttcg ttcccagatg
     1561 catcgccata aaaccaatta agccagaaaa agcaccatag gctttatcct ctttatcttt
     1621 taataatcct aagggaatcg ctatcgcaaa caatacaggt aaattaacaa aggcaaacaa
     1681 accaagacta acaatgaaat caagtatggt tttaattatt ggaatagcca gaaatggaat
     1741 taactttgcc atatcatcac tggctaaacc acttcccagc cctagcatca tgccacatac
     1801 acttagcaga gcaatgggat acataaatgc cttccccagg ctctgaaaaa aactccaggc
     1861 tttcttttgt ttcatgtggg
//
LOCUS       DAZGAP010000385         1832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGAP010000385 DAZGAP010000000
VERSION     DAZGAP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.387"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1045
                     /locus_tag="ACHLAM_005293"
     CDS             <1..1045
                     /locus_tag="ACHLAM_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO7856647.1"
                     /translation="EKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGP
                     AGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKA
                     VKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVAWLK
                     EKGAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVTIGEYG
                     GDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQWGSI
                     EVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPHAIKMYTLSNPSLVSVTLITVR
                     GCYGKFSWEAIGY"
     gene            1055..1279
                     /locus_tag="ACHLAM_005294"
     CDS             1055..1279
                     /locus_tag="ACHLAM_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7856648.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            1279..>1832
                     /locus_tag="ACHLAM_005295"
     CDS             1279..>1832
                     /locus_tag="ACHLAM_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO7856649.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKSGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQA"
BASE COUNT          459 a          387 c          633 g          353 t
ORIGIN      
        1 cgagaaaggt gatccgggag gtcctgcagg cccgcaaggt cctaaaggtg aacgaggaga
       61 agccggacca cagggaccga tgggagcacg aggtgagcgt ggggagactg gcccccgagg
      121 tgaacctggt cctgcaggtc cgagaggcga acgaggagag accggacctc agggacctcg
      181 tggagagcca ggtccggcag gcagcgctgc aaatgtggct gatgcgacga cggcacagaa
      241 gggaattgtg cagttaagca gcgcaacgga cagtgatgat gaaacgaagg cagccacccc
      301 gaaagcggtg aaagcggcaa tggacaaagc ggacggatgc ctggagaaag cgaaaaacgg
      361 tgacgatatc ccggataagg tgaagtttct gaacaccgtg ggggcagcca gagtatacgg
      421 gcgggacatt catacggggg ccggtgaatg gaccacgagc gagtttgtgg cctggctgaa
      481 agaaaagggg gcatttgacc agccttactg gatgatgaag gcatcactgc ttgcggaatt
      541 taataaggtc atcacggatg tcggaccggg aaaactcaat ctgggaggct gcgccattga
      601 ggtgatgggg acgtataacg cagccatagt ccgggtcacc atcggcgaat acggtggcga
      661 tggttttctg aacggcacgg tctgtacctg tacagtttac ggagacacac aacgttttca
      721 ctggcgggtg gattacagca caaaaaacaa gcccacaaca gcaagcctga cagtaaatgg
      781 ctgggagcgt gatgaagtaa ccggtcgttt gcggcaatgg ggcagtattg aggtaagtga
      841 ggatgatggt aagcttatga cacagcactc tatccgtttt cctgtcgctt ttccggttgc
      901 tgctctgaat gctcaggtgt ctgcagtggg agacccccac gccattaaga tgtataccct
      961 gtcgaatccg tcattagtgt ctgtaactct gataacagtc aggggatgct acggcaagtt
     1021 ttcatgggaa gcgatcggat actgagggca ttgtatggaa cagtatgttt tttcaccgtc
     1081 agagaatatg ttctatcccc tgtccctgcg gccggtttat gaggcagcag ggcgctggcc
     1141 ggaagacggt attgtggtgg attatgtggt gtataaagtc tttgcagcgg atgctgcacc
     1201 ggcaggtatg aagcggggcg tcggtgcgga aaaaatgccg gtctgggtgc cggtatcgga
     1261 ggagggcacg gaaacatgat gcatatcaga aatttttcgt attacacccc ggcggagccg
     1321 gatgttgcag gcgcgatgta cctgaagtct gaggatggtc aggactggta tgagtgtcag
     1381 gcgttgtttt cgccggagac gctgaaggtg gtgtatgaca gccggggtgt gattacggga
     1441 tacggtaagg acactgcgtt actgtggccg gtgaaccaga gtgtggcaga agtgccggat
     1501 accccggaga gccggaaaat tgacctgagc gggcgctggg gttttgacgg ggagaaaatc
     1561 acggacctgc tgaccgcgga gaaagcgcga gggatgaagg gcgatgaaat aaacgcgtgg
     1621 cgtaatgcga tggaagcggc gaactacacg tttgagcaca atgggcgtaa atgggactac
     1681 gggaagtcaa cgcagacgcg tcttgagccg tcggtggcgg cagcgaaatc ggggaaactg
     1741 ccggaggcgt ttttctggac ggatgcggaa aacaatgatg tggaggtgac agcagaagag
     1801 cttatagcgc tgagtgaagc ggcagagcag gc
//
LOCUS       DAZGAP010000386         1827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGAP010000386 DAZGAP010000000
VERSION     DAZGAP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.388"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..115
                     /locus_tag="ACHLAM_005296"
     CDS             <1..115
                     /locus_tag="ACHLAM_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7856650.1"
                     /translation="GQEILLQVNATRLQPDIGEHYYLEIHPYGMFVLADAA"
     gene            314..1687
                     /locus_tag="ACHLAM_005297"
                     /pseudo
     CDS             314..1687
                     /locus_tag="ACHLAM_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          441 a          471 c          495 g          420 t
ORIGIN      
        1 tggtcaggag attttattgc aagtcaacgc cacccgttta cagcccgata ttggtgagca
       61 ctattacctc gaaatccacc cttacgggat gtttgtttta gcggatgcgg cataaaaata
      121 gcgcataagc ggtcccctcc agctcagctg tctcttatac acaaatcacc acctggggat
      181 ttgtgatgtc tgtcagtctg ctttaggggg gactctttcc atccggtagt ttccttcctc
      241 cccggcagcg attttttgaa ctttttcttc acccgttgat cttctcttat atttcatttt
      301 cttcgggtct gttatggtac tttctgattg ttattcatgg gctaacgagc agtctggaca
      361 tgcccgtctc ggtgatccgc gtcgtacacg acgccttgtg tcactggcct cttctctcgc
      421 tcagcatgca ggtctttcta ttgtgaaata atcccactcc actgctcagg ttgaaggtgc
      481 gtaccgcctg atgcgtaacc cgtcagtgtc cccggaagcc atttccggag ccggatttac
      541 cgccactgca agggcatgtg aggcacatcc gctgctcccc gcactggaag acacctccac
      601 cattaacttc agccacagta cggcatctga tgacctgggg aataccacca ctaacccgaa
      661 gacgcgcggg ctgctggctc actctgtcct gatatacgcc cccgacagcg ccctgccagt
      721 cgctcttatc gaacagcaga gctggagtcg ggccacggac acctacggtg ttaagcacca
      781 gcgaaaagaa cgtccctgtg aagaaaaaga aagttaccgc tggcagcagg cttcggagcg
      841 catggccgaa cggctggggg agatacagaa acgggtaatc actgtctgtg accgggaagc
      901 ggatatctgg cattacctgc attataaagt cagccacgga cagcgcttcg tggtgcgtgc
      961 tgcacagaat agccggctgg aagaagcacc cggcaagctc ttcgaactgc cggaagtcct
     1021 ggcaaccgcg ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgcca
     1081 ggcccggatg ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca
     1141 ggcgctctcg ctcacgtatg tctgttgccg ggagcaggca gaggacagtg cctgctggca
     1201 tctgctgacg tcagaaaaag tggcgagtgc cgccgatgcg cgacgtatcg tcagccatta
     1261 cgagcgacgc tggctgatag aggaatacca caaggcgtgg aaaagtggtg gtacatgtgt
     1321 ggaatcgctg cgaattcaga cccgggataa ccttgagcgc atggtggtta tcaaggcgtt
     1381 tatcgcggta cgggtgccgg gcctgcggca gggaggtgtc agtgaagaaa cacaaaatga
     1441 cagttgtgag aaaatactga cgccgacaga gtggaaactg ctgtgggtta agctggaagg
     1501 agagccgctt ccggcgcagg caccgatcct gaaatgagcc agtctgaaac tggctaaact
     1561 ggggcgatga catgacagca aacgcacagg ccgccccggt tgggtagtta tgtgggatga
     1621 ctggttcaga cttcaggata tggttgaggg ttacctggtg atgaagtctc ttgatcagga
     1681 tatctgatca agagacagcc agctcagagg ggacatcatc tacttatttc agacataatt
     1741 attttctgtt atcctgtttt acagaataac atataaaaac tttctcaagg caattaccag
     1801 ctatctttca gaaaagttat gacataa
//
LOCUS       DAZGAP010000387         1815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGAP010000387 DAZGAP010000000
VERSION     DAZGAP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.389"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1815
                     /locus_tag="ACHLAM_005298"
     CDS             <1..>1815
                     /locus_tag="ACHLAM_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO7856651.1"
                     /translation="TTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGE
                     QWVHGGGKAAGTIINLDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAES
                     TTINKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLIN
                     RDGWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLG
                     AFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVL
                     LADSGAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARG
                     GSLAGTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVS
                     NTTLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGA
                     TWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDM
                     AQNNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVIEAINGATTEEGAFVQ
                     GNMLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASM"
BASE COUNT          452 a          487 c          561 g          315 t
ORIGIN      
        1 accactgtca ccgcaaatgg tcgtcagatt gtgcaggcag gaggaactgc cagtgatacg
       61 gttattcgtg atggcggagg gcagagcctt aacggactgg cggtgaacac cacgctggat
      121 aacagaggtg agcagtgggt acacggggga gggaaagctg ccggtacaat tattaacctg
      181 gatggttacc agaccataaa acatggcgga ctggcaaccg gaaccatcgt caacaccggt
      241 gcagaaggtg gtccggagtc tgaaaatgtg tccagcggtc agatggtcgg agggacggct
      301 gaatccacca ccatcaacaa aaatggccgg caggttatct ggtcttcggg gatggcacgg
      361 gacaccctca tttacgccgg tggtgaccag acggtacacg gagaggcaca taacacccgg
      421 ctggaggggg gtaaccagta tgtacacaac ggtggcacgg caacagagac gctgataaac
      481 cgtgatggct ggcaggtgat taaggaagga ggaactgccg cgcataccac catcaaccag
      541 aaaggaaagc tgcaggtgaa tgccggcggt aaagcgtctg atgtcaccca gaacacgggc
      601 ggtgcactgg ttaccagtac agctgcaacc gtcaccggta caaaccgcct gggagcattc
      661 tccgttgtgg cgggtaaggc tgataatgtc gtactggaaa atggcggacg tctggatgtg
      721 ctgagcggac acacagccac taatacccgt gtggatgatg gcggaacgct ggatgtccgc
      781 aacggtggtg ccgccaccac cgtatccatg gggaatggcg gtgtactgct ggccgattcc
      841 ggtgccgctg tcagtggtac ccggagcgac ggaacggcat tccgtatcgg aggcggtcag
      901 gcggatgccc tgatgctgga aaaaggcagt tcattcacgc tgaacgccgg tgatacggcc
      961 acggatacca cggtaaatgg cggactgttc accgccagag ggggctcgct ggcgggcacc
     1021 accacgctga ataacggtgc cacgcttacc ctttccggga aaacggtgaa taacgatacc
     1081 ctgaccatcc atgaaggtga tgcactcctg cagggaggcg ctcttaccgg taacggcagg
     1141 gtggaaaaat caggaagtgg cacactcact gtcagcaaca ccacactcat ccagaaaacc
     1201 gtcaacctga atgaaggcac gctgacgctg aacgacagta ccgtcaccac ggatgtcatc
     1261 gctcagcgcg gcacggccct gaagctgacc ggcagcacag tgctgaacgg tgccattgac
     1321 cccacgaatg tcaccctgac atccggtgcc acctggaata tccccgataa cgccacggtt
     1381 cagtcggtgg tggatgacct cagccatgcc ggacagattc atttcacctc cgcccgcaca
     1441 gggaagttcg ttccgacaac cctgcaggtg aaaaacctga acgggcagaa tggcaccatc
     1501 agcctgcgtg tacgcccgga tatggcgcag aacaatgctg acagactggt cattgacggt
     1561 ggcagggcaa ccggaaaaac catcctgaat ctggtgaacg ccggcaacag tggtacgggg
     1621 ctggcgacca ccggtaaggg tattcaggtg attgaggcca ttaacggtgc caccacggag
     1681 gaaggggctt ttgtccaggg gaatatgctg caggccgggg cctttaacta caccctcaac
     1741 cgggacagtg atgagagctg gtatctgcgc agtgaagaac gttatcgtgc tgaagtcccc
     1801 ctgtacgcct ccatg
//
LOCUS       DAZGAP010000388         1804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGAP010000388 DAZGAP010000000
VERSION     DAZGAP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.390"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..652
                     /locus_tag="ACHLAM_005299"
     CDS             149..652
                     /locus_tag="ACHLAM_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO7856652.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(730..981)
                     /gene="yecJ"
                     /locus_tag="ACHLAM_005300"
     CDS             complement(730..981)
                     /gene="yecJ"
                     /locus_tag="ACHLAM_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO7856653.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1096..1182)
                     /gene="azuC"
                     /locus_tag="ACHLAM_005301"
     CDS             complement(1096..1182)
                     /gene="azuC"
                     /locus_tag="ACHLAM_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO7856654.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1445..1768
                     /gene="yecR"
                     /locus_tag="ACHLAM_005302"
     CDS             1445..1768
                     /gene="yecR"
                     /locus_tag="ACHLAM_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO7856655.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          456 a          427 c          408 g          513 t
ORIGIN      
        1 tcagacgtta ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac
       61 cggcagaaag gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc
      121 tccacttctg gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa
      181 ctctcaaatg aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg
      241 ttctgaacag agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt
      301 gacccaaatg atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa
      361 agccattgat gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat
      421 ggaagaatac gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact
      481 gaatgatgat tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg
      541 cctgttgctg caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc
      601 tgtgcagacc gaccaacatg ttctgaatgt cgtgtcgcat cagctacatt gatcatcatc
      661 ggcgctaatg cattgcgccg atgaagtttt tgagaaaccg ctgcctcatt tttttgaagc
      721 agcggttttt taatgggatt cgccctgtgg ggtaaacttg agttcaatca acgcgatggc
      781 tttttggatt gcccgcatgg tgaccgggtc tgcggcggcg ggatggttag taaagtcgat
      841 attctttagc tggctggaca ttttttcgcg aacttcaacg ggtgcgatca catcgagaac
      901 atccagaatt tgtttgataa ccaactggca agcaaccaca tcagaaacca gttcctgatc
      961 ggcattcagc ggctgggaca tcgtaaactc ctgatagcat tttgaaagcc gttatagtag
     1021 cggcttcaca tcttcagcga tagtcacatc caccgtcatc aggacacaaa aaaacctgcc
     1081 ggagcaggtt ttttgttatc ggaacatatt gcctggcggt acgtctttga acgtcttgca
     1141 atagttattg aacatacttt tcaggatttt gcgcagtttc atcgcggcac tccgaccatt
     1201 tgttatacag gtgttattgt ctttgcgctt ataatgtgac aaccatcaca aaaatcaatc
     1261 tttatgtgat gcaaatcaca taaatacccc tttaatgtta taaaaatgat aatcaaaaag
     1321 cagctacccc atttctgaca cctacagatg gcaagaaata gcgcctgcca ggcgtctttt
     1381 ccggccattg tcgcagcgct gtagcgcgta aaatagtgct ttcttttact cttctggctg
     1441 gaccatgaga cttctgattc tgactctttc attaataacg ctggcggggt gtacggttac
     1501 tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgttcggt tggtttatga
     1561 tcaggctttt ttgcagcatg cccgtaccga tcgttatgtc agtcgcggga tcgccgatcg
     1621 cgcatgccag caggcaggct atacccacgc tatccccttt ggtcagccag taggcaactg
     1681 tagcctcttt gcgggttcct tatgtttgaa tacggaattc actttgtcat atcagtgcca
     1741 tcattccgcc ttccctgtct ttctctaaac gttttaatca gcgaggggga tcttcgctga
     1801 ttaa
//
LOCUS       DAZGAP010000389         1792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGAP010000389 DAZGAP010000000
VERSION     DAZGAP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.391"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(118..285)
                     /locus_tag="ACHLAM_005303"
                     /pseudo
     CDS             complement(118..285)
                     /locus_tag="ACHLAM_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            435..1103
                     /locus_tag="ACHLAM_005304"
     CDS             435..1103
                     /locus_tag="ACHLAM_005304"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7856656.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGIVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1469..1582
                     /locus_tag="ACHLAM_005305"
     CDS             1469..1582
                     /locus_tag="ACHLAM_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO7856657.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            1651..>1792
                     /gene="ydfK"
                     /locus_tag="ACHLAM_005306"
     CDS             1651..>1792
                     /gene="ydfK"
                     /locus_tag="ACHLAM_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO7856658.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGN"
BASE COUNT          574 a          387 c          316 g          515 t
ORIGIN      
        1 gaaaatcaat tgataaaata catctaaaca accttttggg gcgcaaaagc ataacatcaa
       61 acaaataaca caccgaaaaa actcacaatt aataacctat gatatacata ctgtttatta
      121 tggttgaata agccactcga tatctggtgc tacggaagtg tccacacggt ttagcagcac
      181 ccgatacttt tttcaggctt ccagcaacga tctttcttcc tccgttgcga tttccagatc
      241 tacagcatcc tgcagtggcg caatatactc actgaattcc tggatgtaga actgtgtggt
      301 gacggtcttc cagctattcg gctcctgctg tattgaagca taccaggcta tttcaatatc
      361 gctatgctgc ggcagcattt aaccccttgt aattcattgc cataattgat ttaattcaca
      421 aataaaacta taacatggtg aaattaatga aaaaaaacac agatgatggg gctaaaattt
      481 acacaccact taccctaaag ctttatgact ggtgggtttt gggagtatca aatcggcttg
      541 catggggatg tcctacaaag gaacaccttc ttccacactt tctggaacat ttaggtaaca
      601 accatctgga tattggtgtt ggaactgggt tttaccttac tcacgtacct gagagtagtc
      661 tgatatcttt aatggatttg aacgaagcta gcctgaacgc ggcatctaca agggctgggg
      721 aatcaaaaat taaacataaa attaaccatg atgtttttga accttatccc gcggcgttac
      781 atggtcaatt tgattccatt tccatgtttt accttcttca ctgcctgcct ggaaatatat
      841 ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt aactgacgat ggaactctat
      901 acggagccac aattcttggc gatggaattg tgcacaatag cttcggtcaa aaactgatgc
      961 gcatttacaa tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa ggcttaacac
     1021 atatactctc agagcatttc gagaatgtta aaaccaaggt tcaaggtact gtagtaatgt
     1081 tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg ttcttgcata aggcgtgtct
     1141 gcggtttttc aactattcag atacatcact cccatcacat tcattcctcc gcatcatagg
     1201 catataggct atatcacctt gatatttttc ttcttcagat aaaaactgtt atctatgtat
     1261 acttttaaac ccaatccgtg tagagtctct gcataagata gtttgcagtt gacacttcag
     1321 cttgcgccat aaaccgcctg atttttgccg ccacctgtta gcattcctgt atacctgaaa
     1381 cgacaatgtt tatctacgaa ctttaagaac acccaggata aaaattgtca actatatcat
     1441 atataacaca ttactaattc gaggctatat gaacagcata ctgataatca cttcgctcct
     1501 tatcatattc agcattttta gtcatgctct aataaaatta gggattggca tatccaataa
     1561 cccagacaaa accgatgtat aagtcaacat atcctgaatc agacatacaa tatcgcaatg
     1621 aaaatcaata atattttaag gaatatcttc atgaaatcaa aagacaccct aaagtggttc
     1681 cctgcgcagc ttcctgaagt aagaattatc ctaggggatg ctgtagtgga agtagcaaaa
     1741 cagggaagac ctatcaatac cagaacattg cttgattaca ttgaaggaaa ca
//
LOCUS       DAZGAP010000390         1790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGAP010000390 DAZGAP010000000
VERSION     DAZGAP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.392"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            171..1754
                     /gene="yegH"
                     /locus_tag="ACHLAM_005307"
     CDS             171..1754
                     /gene="yegH"
                     /locus_tag="ACHLAM_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7856659.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
BASE COUNT          420 a          427 c          475 g          468 t
ORIGIN      
        1 tatttcttaa ataatgtttt attattacca cttttaattc agggataatg tgaggttata
       61 aacctcaaat aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc
      121 atgatcgaat tgtgacattt gtcatacaac gaataggttt tgtacttact atggaatgga
      181 ttgccgatcc gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg
      241 gcattgataa cctggtcttt attgccatcc tcgccgaaaa actaccgccg aagcagcgcg
      301 accgcgcacg ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctggcgt
      361 caatctcctg gctggtcacc ctgactcaac cgctgttcag cttccgctcg tttaccttta
      421 gcgcccgcga cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg
      481 agctgaacga acggctggaa gggaaagaca gcaataaccc cacccaacgc aaaggtgcga
      541 agttctgggg ggtggtgaca caaattgtgg tactggacgc catcttctca ctcgactcgg
      601 tgattaccgc cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg
      661 ctatcagcct gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga
      721 ccatcgttat tctctgccta agtttcctgt tgatgattgg ctttagcctg gtggcagaag
      781 gtttcggctt cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga
      841 tcgaggcgct caatcagttg gctatcttta accggcgacg ttttctttcc gctaaccaga
      901 cactgcgcca gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg
      961 cggaactgga cgccgaaacc gcgtctatgc tggtggatca tggtaaccag cagatcttta
     1021 atccgcagga acggcggatg attgagcggg tacttaatct taaccagcgt accgtcagca
     1081 gcattatgac gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa
     1141 tccgccagtt actggagcga aatcagcata cgcggcttgt cgttactgat ggcgatgacg
     1201 cagaagattt gctcggtgtt gttcacgtta tcgacctgtt acaacagtct ctgcgcggcg
     1261 aaccgctcaa cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt
     1321 tgttacctgc cctggagcag ttccgtaatg cccgcacaca ctttgctttt gtggtggatg
     1381 agtttggctc ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgccggta
     1441 acttaccgaa cgaagtggaa gagatcgacg cccgccatga tattcagaag aatgcggacg
     1501 gttcctggac ggcgaatggt catatgccgc tggaagatct ggtgcaatat gtgccgctac
     1561 cgctggatga gaaacgcgaa tatcacacca ttgccgggct gttgatggaa tatttgcaac
     1621 gtattccaaa acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg
     1681 tggaaagcca tcgcgtgcag aaggtgcaga ttattccgct gcataaggat ggcgagatgg
     1741 agtacgaggt gtgataaaac atcaaatgcg caggttcaaa ctgcgcattt
//
LOCUS       DAZGAP010000391         1782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGAP010000391 DAZGAP010000000
VERSION     DAZGAP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.393"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(120..1403)
                     /gene="gltA"
                     /locus_tag="ACHLAM_005308"
     CDS             complement(120..1403)
                     /gene="gltA"
                     /locus_tag="ACHLAM_005308"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO7856660.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1609..1716)
                     /locus_tag="ACHLAM_005309"
     CDS             complement(1609..1716)
                     /locus_tag="ACHLAM_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856661.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT          470 a          410 c          429 g          473 t
ORIGIN      
        1 cttattaatg ggtatttgca tctggtttaa tttttacaat ttattatgtt cagccatata
       61 aaaaagaacc cgctctatta aaggcgggtc cggaaagtaa acggcttagc aatcaaccat
      121 taacgcttga tgtcgctttt aaagtcgcgt ttttcatatc ctgtatacag ttgacgcgga
      181 cgggcaatct tcataccgtc actgtgcatt tcgctccagt gggcgatcca gccaacggtg
      241 cgtgccatag cgaagatcac ggtgaacata gaagacggaa tacccatcgc tttcaggatg
      301 ataccagagt agaaatcgac gttcggatag agtttcttct cgataaagta cgggtcgttc
      361 agcgcgatgt tttccagctc catagccact tccagcaggt catctttggt acccagctct
      421 ttcagcactt catggcaggt ttcacgcatt acggtggcgc gcgggtcgta atttttgtac
      481 acgcggtgac cgaagcccat caggcggaaa gaatcatttt tatctttcgc acgacgaaca
      541 aattccggaa tgtgtttaac ggagctgatt tcttccagca ttttcagcgc cgcttcgtta
      601 gcaccaccgt gcgcaggtcc ccacagtgaa gcaatacctg ctgcgataca ggcaaacggg
      661 ttcgcccccg aagagccagc ggtacgcacg gtggaggtgg aggcgttctg ttcatggtca
      721 gcgtgcagga tcagaatacg gtccatagca cgttccagaa tcggattaac ttcatacggt
      781 tcgcacggcg tagagaacat catattcagg aagttaccgg cgtaggagag atcgttgcgc
      841 gggtaaacaa atggctgacc aatggaatac ttgtaacaca tcgcggccat ggtcggcatt
      901 ttcgacagca ggcggaacgc ggcaatttca cggtgacgag gattgttaac atccagcgag
      961 tcgtgataga acgccgccag cgcgccggta ataccacaca tgactgccat tggatgcgag
     1021 tcgcgacgga aagcatggaa cagacgggta atctgctcgt ggatcatggt atgacgggtc
     1081 accgtagttt taaattcgtc atactgttcc tgagtcggtt tttcaccatt cagcaggatg
     1141 taacaaactt ccaggtagtt agaatcggtc gccagctgat cgatcgggaa accgcggtgc
     1201 agcaaaatac cttcatcacc atcaataaaa gtaattttag attcgcagga tgcggttgaa
     1261 gtgaagcctg ggtcaaaggt gaatacacct tttgaaccga gagtacggat atcaataaca
     1321 tcttgaccca gcgtgccttt cagcacatcc agttcaacag ctgtatcccc gttgagggtg
     1381 agttttgctt ttgtatcagc catttaaggt ctccttagcg ccttattgcg taagactgcc
     1441 ggaacttaaa tttgccttcg cacatcaacc tggctttacc cgtttgttat ttggctcgcc
     1501 gctctgtgaa agaggggaaa acctgggtac agagctctgg gcgcttgcag gtaaaggatc
     1561 cattgatgac gaataaatgg cgaatcaagt acttagcaat ccgaattatt aaacttgtct
     1621 accactaata actgtcccga atgaattggt caatacttcc acactgttac ataagttaat
     1681 cttaggtgaa ataccgactt cataactttt acgcattata tgcttttcct ggtaatgttt
     1741 gtaacaactt tgttgaatga ttgtcaaatt agatgattaa aa
//
LOCUS       DAZGAP010000392         1769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGAP010000392 DAZGAP010000000
VERSION     DAZGAP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.394"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            266..514
                     /locus_tag="ACHLAM_005310"
     CDS             266..514
                     /locus_tag="ACHLAM_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7856662.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            530..>1769
                     /locus_tag="ACHLAM_005311"
     CDS             530..>1769
                     /locus_tag="ACHLAM_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO7856663.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDK"
BASE COUNT          545 a          423 c          370 g          431 t
ORIGIN      
        1 cccttcagca tcaataatgt gactgagctc attataacgt tgatcccaga atctttgatg
       61 cgctccaaaa tcagtactct tccgcttatc tctcggactc tctccttcct taaacaactc
      121 aaaaactgta caaatatcat acaaaagatt tgtaaacatg cgcatctgct ctgatctccg
      181 gttggtttcc gttttatcac aggggacatt ctggccttaa cctagcaaat taatgacata
      241 gccttcaact cacatggaga acaatatgtc aacccagacc ggacattttt ataccccctc
      301 acgcatcctg cgtatggctg aacttacccg gctccttggt atatcaagat cgaccatcta
      361 cgaaaaaatg aatccggcat caaaatacta cgatgccacc tttccccggc ctgtccggct
      421 tgggagttcg gctgtaggct ggcgggcatc tgccattgat gagtggttaa ctttacatac
      481 aacaccaacc agatccgtaa ctaaggataa ataaggaaaa ctgtacgcca tgacaaaatt
      541 atccccccct gaacttgaca taactaaact gcctgacagg tgccaggcat tacttgatga
      601 aatgcatgaa gaaacgggaa taagccgtga aatactgctg tctattatgc tgacggtcaa
      661 ggctgcctcg gtccaggata cgcatgaagt tgaactttct ggagggcagc gtaccagtct
      721 tcagatatat atgtgtcttt catcagcatc aggcagtgga aaaacctctg cctgcacaaa
      781 attaatcgcc cctgttcatg aaacagaaga agaactacat caggcatata tcgacgataa
      841 aaaaaactat gatcgcatga tggaaatgtg gacaacagat aaaaaaatcc tggaacggag
      901 atataaaaaa gaaatggaaa gatccccgga aaatgctgct gctgcacgag cagcgcttga
      961 aaagtgtata gcaaataaac ccgtacctcc cgtacaacag gttctcatcg tgaatgacgc
     1021 gacaccagaa gggatagctt tgaaactcag ccagtcacct tctcttctgt tgctgtctga
     1081 tgaaggagga actattctgg ataagcgttt cgaacgcaaa tcggcgctgt acaacacatt
     1141 atggagtggg caaccagtca ccgtagaacg ggcatccaga cccgggttcc gggtaaagga
     1201 ttcccgtctc actatgctta tactcactca accggtaata tttgataagt tttttactct
     1261 cactggcgac cagatccggg gcaatggctt tctggcccgg gtgctatttt gcgaacccgg
     1321 agcaaataaa ataatgacga cagaaccgca tactgccact cctgttgtaa cgcagcaatg
     1381 tgcaacctgt tttcatgaga aaagttttgg cagtcagatc agagactctc tgagagcgtc
     1441 ccgggaaagg cgtgcaaaag gtgagcaacg catctgcatg acattatcaa acgcagcatc
     1501 ccacgatctg gaaatctttc acaaagaaaa tatgagtgca gtcaggcaga acccacgtat
     1561 gaccactttc gaagacatta tcgtcaggaa aagagaacag gctgttcgaa tagccgcact
     1621 tctggagctg gagaacaatc cacatggtac agtaataaca cgagaaagta tcaatagtgc
     1681 tatttatctc gttgattttt attttcagca tcttatatcc aaactggaat cacttcggga
     1741 aatatctcct gcagaaaaac tggataaat
//
LOCUS       DAZGAP010000393         1766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGAP010000393 DAZGAP010000000
VERSION     DAZGAP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.395"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..126)
                     /locus_tag="ACHLAM_005312"
     CDS             complement(<1..126)
                     /locus_tag="ACHLAM_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856664.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWP"
     gene            204..1298
                     /gene="ddlA"
                     /locus_tag="ACHLAM_005313"
     CDS             204..1298
                     /gene="ddlA"
                     /locus_tag="ACHLAM_005313"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7856665.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1501..1713)
                     /gene="yaiZ"
                     /locus_tag="ACHLAM_005314"
     CDS             complement(1501..1713)
                     /gene="yaiZ"
                     /locus_tag="ACHLAM_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO7856666.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
BASE COUNT          455 a          433 c          442 g          436 t
ORIGIN      
        1 tggccataca agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcgcac
       61 atcattgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg
      121 caacattctg tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
      181 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
      241 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
      301 aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
      361 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
      421 cgaccagcct tgcgcaagtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg
      481 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
      541 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
      601 tggcttcagc agcctgtatg gacaaagatg tcaccaaacg tctgctgcgc gatgccgggc
      661 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
      721 aagtggagtc taaactgggg ttaccgctgt ttgtaaaacc ggctaatcag ggctcttctg
      781 ttggtgtcag caaagtaacc agcgaagaac agtacgcaat tgccgtcgat ctggcgttcg
      841 agttcgatca taaagtgatc gttgagcaag ggataaaagg tcgtgagatc gaatgcgcag
      901 ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt
      961 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     1021 ccattgcgcc agaaatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     1081 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     1141 tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     1201 aagccagcgg tctgggttac accgacctga tcacgcgttt gattgagctg gcgctggagc
     1261 gtcacgctgc ggataacgca ttgaaaacca caatgtaatt tttaaatgcc tgataacttt
     1321 tattgggcat ttaagactga tggcaaacgt aagattgcct gatgcgcttt gcttatcagg
     1381 cctaccagat aattgcaata tactgatttt gcatgatttt gtaggcaggg taaggcgttc
     1441 gcgccgcatc cggcaaaaac aacgagcact ttgtcagccc ccagaacgcc caatcctctg
     1501 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcagccccg ccagccagaa
     1561 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     1621 cagtaaaccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     1681 ccagtcacgg cggattttta caggcaagtt catggcagct ccgttaacga aaaacaacat
     1741 ccttgcatat tctgcaacat ccgtct
//
LOCUS       DAZGAP010000394         1735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGAP010000394 DAZGAP010000000
VERSION     DAZGAP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.396"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            35..568
                     /locus_tag="ACHLAM_005315"
     CDS             35..568
                     /locus_tag="ACHLAM_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7856667.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            866..1333
                     /locus_tag="ACHLAM_005316"
     CDS             866..1333
                     /locus_tag="ACHLAM_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7856668.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     EQANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATSTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAKEYIRTQCIN"
     gene            1357..1581
                     /locus_tag="ACHLAM_005317"
     CDS             1357..1581
                     /locus_tag="ACHLAM_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856669.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          439 a          404 c          491 g          401 t
ORIGIN      
        1 gggattcgga accgataaat cctgataaat atccatgaac accaaaatca aatacggcct
       61 gtcggctgcc gttctggcgc tgattgccgc aggtgcgcct gcgcctgaaa tcctcgacca
      121 gtttctggat gaaaaagaag gcaaccacac cacggcatac cgtgatggcg cgggtatctg
      181 gaccatctgc cggggtgcca tcatggtgga tggtaagcct gtgattcctg gcatgaagct
      241 gtcgaagggg aaatgcgacc gggttaacgc cattgagcgt gataaggcgc tggcatgggt
      301 ggagaaaaac atcaaagtgc cactgaccga accccagaaa gcggggatcg cgtcattctg
      361 tccttacaac atcggcccag gtaagtgttt tccgtcgacg ttttataaac gaattaatgc
      421 aggcgatcgc aggggagcgt gtgaagcgat tcgctggtgg attaaggacg gtggcagaga
      481 ctgccgtatc cgctcaaata attgctacgg tcaggtatcc cgtcgcgacc aggagagtgc
      541 gctggcatgc tggggtatcg acaggtagta gaatattttg ctgaaaaatg acgttgacca
      601 acgcggacgg ataacacgaa atcctgcgaa ctggcaaaac ctaagtgaat aaaagtaaaa
      661 accccgtttg ttggcagcaa gcggggtttt gtgtttcctg actccggaaa agtcaaagga
      721 gaaagtgtgt ttgattttag caaactgatt cgggagattc gagtgatggc tgaaaaatta
      781 tccacctgga agttcatcct tatctggctg gtgtttgtga ttatggcctc cggttatttc
      841 atcggtcaga tacgctggtg gtgaaatgaa ccgcgttctg tgtgtggtca tcattgcctt
      901 gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat taccgcgata acgccatcac
      961 ctacaaagcg cagcgcgata aaaaagccag agagctggaa caggcgaatg ccaccattac
     1021 tgacatgcag gtgcgccagc gtgatgttgc tgcgctcgat gcaaaatact cgagggaatt
     1081 agccgatgcg agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg
     1141 cctgcggatc aacgccacct gccacggtac cgtgcgtgaa gccaccagca cctccggcgt
     1201 ggataatgca accggccccc gactggcaga caccgctgaa cgggattatt tcatcctcag
     1261 agaacggttg atgacaatgc agaagcagct ggaaggggcg aaggaatata tccgcactca
     1321 gtgtattaac tagtattttt gttatccgga gaatgcatga agaaattact ggtaaccgta
     1381 gaaccttttc agggaacaat tccgttccgt attttgcagc gtggtcgtgt tcttgttgaa
     1441 ggttcgttca gtggtaaatg tacgcaatta cactcccgga cctttcaggt gaatgccacg
     1501 aatgaagagc taaccgttga gtgtacgatg aatgccgcta aatgccgcat ggtatccgct
     1561 gcattacagc cagtgtgttg agcgacctta ttatccatgc gcggtattgt cgccgtattc
     1621 ccgtattaac agagaccgca gcccgacagg gagactcctc tgcgcgagtg tgcggggata
     1681 atcaaaaacg atacacaccg gggtttaccg cgttaacgga gcgcggcgtt gtccc
//
LOCUS       DAZGAP010000395         1733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGAP010000395 DAZGAP010000000
VERSION     DAZGAP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.397"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..556)
                     /locus_tag="ACHLAM_005318"
     CDS             complement(113..556)
                     /locus_tag="ACHLAM_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7856670.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAAYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            713..1642
                     /gene="metA"
                     /locus_tag="ACHLAM_005319"
     CDS             713..1642
                     /gene="metA"
                     /locus_tag="ACHLAM_005319"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO7856671.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          426 a          480 c          388 g          439 t
ORIGIN      
        1 aaaggccatc cgaaaggatg gcctttttgc attggcgcag aaaaaatgcc ggatgcgacg
       61 ctggcgcgtc tgctccagct tacgcaaaca cgatagcacg atagggggca gcttattccc
      121 ctacatatgc cagattcagc aatggatacg gtttccccaa atcgtccacc tcagagcgtc
      181 ccgtaacctt aaaacccatc ttcttataga acccaacagc ctgcttattt tgctcattaa
      241 cattggttgt cagttccggt gccatcgaga gcgcatgttc caccagcatc cgacccacgc
      301 cgcagccgcg cacatcagga tcgataaaca gcgcatccat atgctgccca cttaacaaca
      361 taaacccaac cggccgatcc cgctcattaa ccgcgaccca caacggcgct tccggcagga
      421 aggaacgaac tagctcctcc agctcggccc gatacgctgc tgataaaaaa tcgtgagtgg
      481 catcgacaga acgacaccaa atcgcaacga gctcctcccc ttcctcatgc cgtgagcggc
      541 gaatactaat aaccattttc tctccttttc gtcattctta tattctaacg tagccttttc
      601 cttgaaactt tctcaccttc aatatgcagg ctcgacattg gcaaattttc tggttatctt
      661 cagctatctg gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg ttatgccgat
      721 tcgtgtgccg gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat
      781 gacaacttct cgtgcgtctg gtcaggaaat tcgtccgcta aaggtgctta tccttaacct
      841 gatgccgaag aagatcgaaa ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt
      901 gcaggtcgat attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga
      961 gcatctgaat aacttctact gtaactttga agatattcag gaacaaaact ttgatggtct
     1021 gatagttacc ggcgcgccgc tgggcctggt ggagtttaat gatgttgctt actggccgca
     1081 gatcaaacag gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgttg
     1141 ggcggtccag gccgcgctca atatcctcta cggcattcct aagcaaaccc gcaccgacaa
     1201 actctctggc gtttacgagc atcatattct ccatcctcat gcgcttctga cgcgtggctt
     1261 tgatgattca ttcctggcac cacattcgcg ctatgctgac tttccggcag cgttgattcg
     1321 tgattatacc gatctggaaa ttctggcgga gacagaagaa ggggatgcct atctgtttgc
     1381 cagcaaagat aagcgaattg cctttgtgac gggccatccc gaatatgatg cgcaaacgct
     1441 ggcgcaggaa tttttccgcg atgtggaagc cggactagac ccggatgtac cgtataacta
     1501 tttcccgcat aatgatccgc aaaatacacc gcgagcgagc tggcgtagtc acggtaattt
     1561 gctgtttacc aactggctca actattacgt ctaccagatc acgccatacg atctacggca
     1621 catgaaccca acgctggatt aatcttctgt gatagtcgat cgttaagcga ttcagcacct
     1681 tacctcaggc accttcgggt gcctttttta tttccgaaac gcacctcagt agg
//
LOCUS       DAZGAP010000396         1707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGAP010000396 DAZGAP010000000
VERSION     DAZGAP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.398"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..622
                     /gene="dsbE"
                     /locus_tag="ACHLAM_005320"
     CDS             65..622
                     /gene="dsbE"
                     /locus_tag="ACHLAM_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO7856672.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKL
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            619..1671
                     /gene="ccmH"
                     /locus_tag="ACHLAM_005321"
     CDS             619..1671
                     /gene="ccmH"
                     /locus_tag="ACHLAM_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO7856673.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKAAPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
BASE COUNT          393 a          422 c          510 g          382 t
ORIGIN      
        1 tgtgcctgtt tgatcctcgc tatcgtaagc gcgtgaaccc gcagaaaact gcgccggagg
       61 ctgtatgaag cgcaaagtat tgttaattcc gttgattatc ttcctggcga ttgccgcggc
      121 gctactgtgg cagctggcgc gtaatgccga aggggatgat ccgaccaatc tggaatcggc
      181 gctcattggc aagctagtgc cgaagtttcg cctcgaatca ctggacaatc cggggcagtt
      241 ttatcaggcg gatgtgctga ctcagggcaa accggtgctg cttaacgtct gggcgacctg
      301 gtgtccgacc tgccgtgcgg aacatcaata tctgaatcag ctttctgcgc agggcatccg
      361 cgtggtcggc atgaactata aagacgatcg ccagaaggca atcagctggc tgaaagagtt
      421 gggcaatcct tacgcactaa gcctgtttga tggcgacggc atgttagggc tggatctcgg
      481 tgtctatggc gcgccagaaa cgttccttat tgacggcaac ggcatcattc gctatcgcca
      541 tgcgggcgat ctcaatcctc gcgtctggga agaagagatc aagccactgt gggagaaata
      601 cagtaaggag gccgcacaat gaggttttta ttgggcgtgc tgatgctgat gatctccggc
      661 tcagcgctgg cgaccatcga cgtgttgcag tttaaagatg aagcacagga acaacagttc
      721 cgtcagctca ctgaagaact gcgctgcccg aaatgccaga acaacagcat tgccgattcc
      781 aactcgatga ttgccaccga cctgcgccag aaagtgtatg aactgatgca ggaaggcaaa
      841 agtaagaaag agattgtcga ttatatggtg gcgcgttacg gcaacttcgt cacttacgat
      901 ccgccgttaa cgccgctgac cgtgctgctg tgggtgctgc cagtagtggc tattggcatt
      961 ggcggttggg tcatatacgc ccgttcgcgg cgtcgggtac gcgtggtgcc ggaagcgttt
     1021 ccagaacaaa gcgtgccgga aggtaagcgt gccggatatg ttgtttatct gccgggtatt
     1081 gtggtggcgt taattgtggc tggcgtcagc tactaccaga ctggcaatta tcagcaggtg
     1141 aaaatctggc agcaggccac cgcgcaggct ccggcgttac tggacagggc gctggatccg
     1201 aaagccgctc cgctcaacga agaagagatg tcgcgccttg cgctggggat gcgtactcaa
     1261 ctgcaaaaaa atccgggaga tatagaaggc tggattatgt tgggccgcgt tggcatggcg
     1321 ctgggtaacg ccagtatcgc caccgatgca tacgctactg catatcgcct cgatccgaag
     1381 aacagtgatg cggcgctcgg atacgctgaa gcgttgacac gttcatctga tcccaacgac
     1441 aaccgcctcg gcggcgaact gctgcgccag ttggtgagaa cggaccatag caatatccgt
     1501 gtgttaagca tgtatgcatt taatgccttt gagcagcaac gatttggcga agccgttgcc
     1561 gcgtgggaaa tgatgttgaa actcttacct gccaacgata ctcgccgtgc ggtgatcgaa
     1621 cgtagtatcg cgcaggcgat gcaacatttg tcgccgcagg agagtaaata aaaagtggcc
     1681 cgatggtgat gccatcgggc tattttt
//
LOCUS       DAZGAP010000397         1656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGAP010000397 DAZGAP010000000
VERSION     DAZGAP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.399"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1288
                     /gene="tssC"
                     /locus_tag="ACHLAM_005322"
     CDS             <1..1288
                     /gene="tssC"
                     /locus_tag="ACHLAM_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7856674.1"
                     /translation="LIDHHIAELDDQISRQLDAVLHHPEFQKVESLWRGVKSLVDKTD
                     FRRNVKIELLDLSKDDLRQDFEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALVSAWEF
                     DASAQDVALLKNISRVAASAHMPFIGSVGPAFFRKETMEEVAAIKDIGNHFERAEYIK
                     WNAFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPDHHKYLWTNASFA
                     FAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVMIPETREFE
                     FANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARLPYIFLLSRVAH
                     YLKMIQRENIGTTKDRRLLELELNTWLRGLVTEMTDPGDELQASHPLRDGKVVVEDIE
                     DNPGFFRVKLYAIPHFQVEGMDVSLSLVSQMPKAKA"
     gene            1304..>1656
                     /gene="tssK"
                     /locus_tag="ACHLAM_005323"
     CDS             1304..>1656
                     /gene="tssK"
                     /locus_tag="ACHLAM_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7856675.1"
                     /translation="MKIFRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     EQVEFNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVANVEQ"
BASE COUNT          350 a          401 c          483 g          422 t
ORIGIN      
        1 gctgattgac catcatattg cagagcttga tgaccagatc agtcgtcagc tggatgccgt
       61 gcttcatcat ccggaatttc agaaagtgga atccctgtgg cggggcgtga agtctctggt
      121 ggataaaacg gacttccgcc gtaatgtgaa aattgagctg ctggatctgt ccaaagacga
      181 tctgcgccag gattttgagg atgcgccgga aattattcag agcggccttt atctgcagac
      241 ctatgtggct gaatatgaca cgccgggtgg tgaacccatt gctgccctgg tttcggcctg
      301 ggaatttgat gcatctgcgc aggatgtggc gttgctgaaa aatatttccc gggtggcagc
      361 atccgcacat atgccgttta ttggttctgt tggtccggct ttcttccgga aagaaaccat
      421 ggaagaggtg gctgctatta aggatattgg caatcacttt gagcgtgccg agtacatcaa
      481 gtggaacgct ttccgagaga cggatgatgc ccgctatatt ggtctggtga tgccccgtgt
      541 gctgggacgc ctgccgtatg gccctgatac tgtacctgtc cgcagcttta actatgttga
      601 agaggtgaaa gggccggatc atcacaagta tctctggacg aacgcgtctt ttgcttttgc
      661 ggccaatatg gtgcgcagtt ttgtcaccaa cggctggtgt gttcagatcc gcggtcctca
      721 ggccggtggt gcggtgcagg atttgccgat ccatctgtat gatctgggaa ccggtaatca
      781 ggtcaaaatt ccgtcagagg tgatgatccc ggaaacgcgg gagtttgaat tcgctaatct
      841 gggttttatt ccgctctctt actacaagaa ccgggattat gcctgtttct tctccgctaa
      901 ctccacccag aaaccggcac tgtacgatac gccggatgca acggcgaaca gccgtattaa
      961 tgcccgtctt ccctatattt tcctgctgtc ccgcgttgcg cattacctga agatgattca
     1021 gcgggaaaat atcggtacca ccaaagatcg gcgtctgctg gagctggagc tgaatacctg
     1081 gctccggggc ctggtgacgg agatgaccga tccgggcgat gagcttcagg catcgcatcc
     1141 gctgcgtgac ggaaaagtgg tggtggaaga tattgaagat aacccggggt tcttccgtgt
     1201 gaaattgtat gccattccgc atttccaggt ggaaggaatg gacgtcagcc tgtccctggt
     1261 ttcgcagatg ccgaaggcaa aagcgtaagg caggtgtcgg gaaatgaaga ttttccgtcc
     1321 gctgtggagc gaaggtgttt tgctctcccc tcagcagttt cagcagcagg ctgaatggga
     1381 gtcttttcgc agtgccggta tctctgcgct ggcatccccg tttccctggg gagtggaaca
     1441 ggttgagttt aatgacagtc ttctgtcgtc cgggctgatc cagattacgc agttacgcct
     1501 ctggctggaa gacggaacac ttattgatgc tcaaaggagc gatctgcctc cggcgccacg
     1561 tgagttagat gccggtcagc tggctgaccg cgatgcggta actgtcgtta ttgcacttcc
     1621 ccacatgcag ccaggcgttg ccaacgttga gcagga
//
LOCUS       DAZGAP010000398         1640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGAP010000398 DAZGAP010000000
VERSION     DAZGAP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.400"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..1637
                     /locus_tag="ACHLAM_005324"
     CDS             6..1637
                     /locus_tag="ACHLAM_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO7856676.1"
                     /translation="MMSTANAADDVKLKATKTNVAFADFTPTEYSTKGKPNIIVLTMD
                     DLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAIEAAKKSTPTLAALMDEGVKFTN
                     GYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGIPLEETFLSELLQNHGYYTAAIG
                     KWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQNRGFNYFMGYHASGVSLYNSPS
                     LFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPFMIYLAYSAPHLPNDQPAPKNYQ
                     EKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQYDNTIILFTSDNGAVIDGPLPL
                     NGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYNKLVSSMDFYPTALDAADIKIPASL
                     KLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSHWWDEENTAFWNGYHKFVRKESD
                     DYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLALYDINDLQQTKDLSASHPDVVKN
                     MKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
BASE COUNT          522 a          359 c          334 g          425 t
ORIGIN      
        1 gtctgatgat gagtactgca aatgcagcag atgatgttaa gctcaaagca acaaaaacca
       61 atgttgcctt tgcggacttc acgccgacgg aatacagcac taaaggtaag ccaaatatca
      121 ttgttttaac aatggatgat ttagggtatg gacaattgcc ttttgatgat agttcatttg
      181 accctaaaac gatggataac cgaaatgttg ttgatacata caaaataggt attgataaag
      241 caattgaagc tgcaaaaaaa tccactccaa cattagcggc cctgatggac gaaggtgtaa
      301 aattcactaa cggctatgtt gcacacggtg tctctggccc ttcacgtgcc gcaatcctga
      361 cggcgcgagc tcctgctcgc ttcggtgtat attcaaattc tgatgcacag gatggtatcc
      421 ctctggaaga aacattcctg tctgaattac ttcagaatca tggttattac actgcagcaa
      481 tcggtaagtg gcacttatca aaaatcagca acgtaggtgt gcctgaagaa aaacaaaccc
      541 gtgactacca cgataacttc accaccttct ccgcagaaga atggcaacct cagaatcgtg
      601 gcttcaacta cttcatgggt tatcacgcat ccggcgtttc actttataac tccccgtcat
      661 tgttccgtaa ccgtgaaaac attaaagctg aagggtacat tagtgatcaa ttaactgatg
      721 aagcaattgg tgtaattgac cgtgcaagca aactggaaca gccatttatg atttatctgg
      781 catacagtgc accacacctg cctaacgatc agcctgcgcc taaaaactat caggagaaat
      841 tcaacactgg tagcaagact gctgataact attatgcgtc aatttactcc gttgaccagg
      901 gggttaaaaa gatcctgaat acacttaagg aaaaaggtca gtacgacaac accatcattc
      961 tgttcacctc tgataacggt gctgtaattg atggtccact gccactgaat ggtgcgcagg
     1021 ctggttacaa aggccaaccg tatccgggtg gtatgcatac acctatgttc atgtggtgga
     1081 atggcaagct gaaaacgggt gattataaca aactggtttc atcaatggac ttctatccga
     1141 ccgctttaga tgccgcagat atcaagatcc ctgcttctct gaaactagat ggtgtatcat
     1201 tattgccata tgtacagaaa ggagctgata agaaatctgc agctggcgaa cctcacaaat
     1261 atctgtcctg gattacctct tattctcact ggtgggacga agagaacact gcattctgga
     1321 acggatatca taaattcgtg agaaaagagt ctgatgatta tccgcacaac ccacatacag
     1381 aagacttaag ccagtttgca tacactgtaa gaagcaacga ttattctctg gtttacacct
     1441 atgcagagaa taaactagct ctgtacgata tcaacgatct gcaacaaact aaagatctct
     1501 ctgcatccca tcctgatgta gttaagaata tgaaggctgt tatgattgac ttcatcacgt
     1561 caagtcaaaa accgatcagt gatatcaacc aagccaaatt tgagaatata aaaaaatctt
     1621 taactaacgt gaaatgataa
//
LOCUS       DAZGAP010000399         1625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGAP010000399 DAZGAP010000000
VERSION     DAZGAP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.401"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(476..1301)
                     /locus_tag="ACHLAM_005325"
                     /pseudo
     CDS             complement(476..1301)
                     /locus_tag="ACHLAM_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          446 a          388 c          340 g          451 t
ORIGIN      
        1 aaaattttca caaagtaaat ttaaaaacaa agaaatttaa tgtaatataa tcgggggaga
       61 gttagccatc gatgattgcc agaacaaaaa acaatataaa ccaacaagat agaaaatcat
      121 cacaacaaag ggaaaggtct gtagataaag agtgggatgg acttcttact tcatatcaca
      181 taattgatat tttttatcct gcaaactcca acggcattat gtatgactta gcctcaggca
      241 attatcacag gcaaaaaaac aatatattgt aagcttcata gaatttggaa ctttatataa
      301 taaataatgc tgagtttttt atcaccaata cgttaacaaa attaatgtat tccagaggtt
      361 tcacattagt ctccaatttt tcagaagaat ctatttacct ggcaggaaaa gtcatctggt
      421 gtttcgccgg agtgaggggt acaggcaata gcggacataa ccatttgttc tttttctaag
      481 cagccatctg ataatatttt atcctgaagg ctgtcgggga gatattcccc agacgagagt
      541 gacgacgctg acgattgtag aaaatctcaa tgtattcccg tattactgag atggcctcat
      601 cccggctttt aaaacgatag tggctcagac tttcattttt cagcgttccc cagaagcttt
      661 ccataggagc attgtcgtaa cagttaactt tacgcgacat tgatattttc agaccaaact
      721 gctcctgtat ggccctgtaa tcgtatgcgc agtactgtga accacgatcg gagtggtgga
      781 ttagcccggt aggtgagcgc tggttcctga gcgccataaa cagggcttta cccgtcaact
      841 atttcgtcat gcgctctccc atggcgtaac cgacaatttt gctcgtataa acatctttga
      901 cgccagcgat atacagccat ccttccgggg ggcaacatac gttatatctg caaccaagac
      961 ctgattaggt gctgtaggcg taaagttctg attcaggaga ttcggcgcaa ccggcagatt
     1021 gtgacaagag ttcgtgttcg ccctgaactt gcgtttctgc ttacagtgca gcctcagctc
     1081 cttacgaaga cgtgccagtc tgtcacgacc gacgataatt ccgttatctg ccagctccgt
     1141 ctggagcagc taggttccat atgtttcgcg tgtgcggata tgtgccacct taatctccag
     1201 ttttagccgc tcatcacttt gttttctgtc tgagggtcat gctgtactca gtcgtaataa
     1261 ccgatcctgt atacaccaag tacccgacac atcgcttcaa tacataatcg catctgaatc
     1321 gttcaccgat tcgcggcatt tttcaaggta gttgtcgcca ttgttcgact aactgatcga
     1381 cattacagcc tagagcctag ctgggggctg tgcacaaaaa agccccggac attccggggc
     1441 tacactgcat attaacgaac gctctgtccg gttatttcaa ccctgcggtt ggggcgcaga
     1501 cattccttca gtgcttcgcc ctccacatcc ggacagaaca ccactggtgc atcatatcca
     1561 cggcccgttg catgtatcct ttcagccctg aatccccggg caataaacca ccccctgact
     1621 gattc
//
LOCUS       DAZGAP010000400         1600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGAP010000400 DAZGAP010000000
VERSION     DAZGAP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.402"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1515
                     /locus_tag="ACHLAM_005326"
     CDS             <1..1515
                     /locus_tag="ACHLAM_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7856677.1"
                     /translation="VRTTTSDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAE
                     DAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVE
                     SRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDHLTTVQTDTTRIQTV
                     YEPGSFTPLIRIETDNGEREKTQRRSLAEKLQQEGSEDGHGVVFPAELVRMLDRLEGE
                     IRADRVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISTEGA
                     TEWRGEYDEWGNLLNEENPHHLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQD
                     PIGLEGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISL
                     GGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSV
                     GVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          404 a          367 c          495 g          334 t
ORIGIN      
        1 gtgcgcacga ccacatcaga cctggatatc cgcatcccgt atgccaccga cccggcggga
       61 aaccgcctgc cggacccgga gctgcacccg gacagtacac tcacagtgtg gccggataac
      121 cgcatcgcgg aggatgcgca ctatgtctac cgccacgatg aatacggcag gctgacggag
      181 aagacggacc gcatcccggc gggtgtgata cggacggacg acgagcggac ccaccactac
      241 cactacgaca gccagcaccg cctggtgttc cacacgcgga tacagcatgg cgagccactg
      301 gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcgaaacg ggtatggcgg
      361 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacgtggtac
      421 ggctgggacg gcgaccacct gacgacggtg cagaccgaca ccacgcgtat ccagacggta
      481 tacgagccgg gaagcttcac accgctcatc cgcatcgaaa cagacaacgg cgagcgggag
      541 aaaacgcagc ggcggagcct ggcggagaag ctgcagcagg aaggaagcga ggacggccac
      601 ggtgtggtgt tcccggcaga acttgtgagg atgctggaca ggctggaggg ggaaatccgg
      661 gcagaccgcg tgagcagtga aagccgccag tggctggcac agtgcgggct gacggtggag
      721 cggctggcgg cacagataga gccggtttat ttacctgagc gaaaaattca tctttatcac
      781 tgtgaccacc gggggctgcc gctggcgctc atcagtaccg aaggagcaac ggaatggcgt
      841 ggggaatatg atgaatgggg caacctgctg aatgaggaga acccgcatca cctgcaccag
      901 ccataccgtc tgccggggca gcagcatgat gaggaatcag gactgtacta taaccggaac
      961 cggtactatg acccgctaca ggggaggtat attacacaag accccattgg gctggagggg
     1021 ggatggaacc tgtataatta cccactgaat ccgataataa ggatggatcc tttgggtttg
     1081 tataatttat atcaattatt atatgatgtt tggcatgatg attcatatgg aacatcatca
     1141 attgatatta ctggcagtgg agatctaata tcattaggtg gtcatgcagg acttggcgtt
     1201 gcgtttgcta aaaagaaagg tgaaatgtta tctgatattt gtatttatgc tacagcatgt
     1261 ggacatgcag gaattggtgg tgggataaat gcggctatca catattctga gaccaagtct
     1321 ttacctacat cgggagtcag taattcagta ggtgtaacgg ttggcggcgg agttgggggg
     1381 cattttgcgt atacttatgt agtggatgtt gataatccag aatcatcgac agaatctgtt
     1441 ggtatcggtg caggtgttga cgcttcagtt atgactctgg cttgtagaac gtggcaagaa
     1501 tgctgggtca attaacagga ggaaatatga aaaaatatat atttttagtt ttatttttat
     1561 tgtgtggttg ttctccagtg gacatagagc cagctagaca
//
LOCUS       DAZGAP010000401         1589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGAP010000401 DAZGAP010000000
VERSION     DAZGAP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.403"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..127)
                     /locus_tag="ACHLAM_005327"
     CDS             complement(<1..127)
                     /locus_tag="ACHLAM_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO7856678.1"
                     /translation="MTRTFTMHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNE"
     gene            complement(127..786)
                     /gene="yjfK"
                     /locus_tag="ACHLAM_005328"
     CDS             complement(127..786)
                     /gene="yjfK"
                     /locus_tag="ACHLAM_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO7856679.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFLRALGVDIPLTSLHII
                     G"
     gene            complement(836..1534)
                     /gene="yjfJ"
                     /locus_tag="ACHLAM_005329"
     CDS             complement(836..1534)
                     /gene="yjfJ"
                     /locus_tag="ACHLAM_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO7856680.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
BASE COUNT          404 a          414 c          335 g          436 t
ORIGIN      
        1 attcgttgtg cggtgtaatt ttggagtaga taaacagaaa aataatcacc atagccacac
       61 caataaaaaa ataagcgcta aaggcaagaa gtgaatccag tatatgcatc gtaaacgtcc
      121 ttgtaattaa ccaataatat gaagtgatgt cagcgggata tcgaccccca gcgcgcgcaa
      181 aaataaccac tccggctcgc cgagatcatt aaaagattcc tcaccattta acagcagata
      241 ttcgtaagta tcttcggtca cttgccgttg atagcccatg gtaaaattat ggacctccca
      301 tttagcatgg ttttgatttt ctactttttc cagcatgtag acgggttcga tatttcccgg
      361 ttcttcgcta ttaaaaaatc gctgccagcg tttttcctgc cagtttaagg tcattgcccc
      421 catcgctttg gcattgatcg cctcgcgcca gtgactctct ttactgatac cgtagctctc
      481 ttcatagaca aagagcttga tatcatcaat gtcatcaata tcttcgccgc cagtggtatt
      541 gatttgcaga aactcatcgc ctgaagtgta gtagcggaaa atctggctac cgccgcccag
      601 atcgatgcgg ctgacagcgg ctaccgtaaa ttcctcaccc ggcagcgcaa tcagcaattc
      661 atcttccagc aaacgaaacg ctaacgtgtc gagcgtaaag ccactattga gatgaagtcc
      721 cagaggaccg cgagcgatag caggcttatt atccttgcca aacagacgct ggaaaaaacc
      781 agacatattt tatccttaaa atagttgcca gccttgcagt gaggctggca aaaaattact
      841 cgccctgttg gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc
      901 caattccggc ttccgccagc ttttcatcaa gatcgcgacc gtctgcgact ttttccaact
      961 gtgcggcagc atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg
     1021 attctgccgc tgtcgaaacg ctggaagatg cgccaacagt agaggtcgtt acagcctgtt
     1081 gtgcacgctg catggcttca gtagctttaa cgacttccat ttgctgctca aactgagcga
     1141 tacgctgcgc tgtcgctgta actgcttttt ccacgccatc acgagagact tccagattcg
     1201 acaacacttg ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt
     1261 cgttaatcaa cgacggatta acgttcttgc ttaacgcttc cagcgcacga gcttccagac
     1321 tggctttgcg ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca
     1381 gatcaacgcg agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt
     1441 ccagcatgcg cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt
     1501 tccccagcgt aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg
     1561 aatattcttc tgtaatttca gccagatcc
//
LOCUS       DAZGAP010000402         1586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGAP010000402 DAZGAP010000000
VERSION     DAZGAP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.404"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..519)
                     /locus_tag="ACHLAM_005330"
     CDS             complement(<1..519)
                     /locus_tag="ACHLAM_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856681.1"
                     /translation="MGLFNYKDYHQAQITGLISASYNIAVYANTGKVLGLGSFLVSRL
                     FAKHPDRIDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSL"
     gene            complement(578..>1586)
                     /locus_tag="ACHLAM_005331"
     CDS             complement(578..>1586)
                     /locus_tag="ACHLAM_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856682.1"
                     /translation="AFYERALPSNVSGDLYPQPSIFGDKVSSVSKNWSTLLDSNPGSY
                     VTSQRLDSGANQYNYNGHTGSDVISITDSLGGLDRTQVFRFPVGLFTGEGNDLIVTGR
                     DYGRNTSAGYTDHSHRTDMGNGDDTLVVGVGNNDVTLYVNEEGQLRATTDSYNGSTSI
                     DYTGLNSSSSGGTISGTDIVMGAGNDTVLALGYEGNSADAIINTNIDLGAGNDFIYAN
                     GEISTNNGTQVNIIGGEGFDTISLDNTTVTSAMFSGFEHVDLHSTSHLILNSDDFKSQ
                     DIEGEILKISGSSGASVDVQNFDWENLGSANDGDVKYFTYQSTDIPGLTLWIQEGIEV
                     K"
BASE COUNT          490 a          359 c          256 g          481 t
ORIGIN      
        1 caggctatag cctgtgacaa taacatcttc accacttaaa ccattactga ttgcaaagtc
       61 tctgatactt tgaagcaggt tttctgcttc agacagcgct tttccattat tgagctgtgt
      121 ataatcaaca atatcgcaga tatcatttgt tccacagaat gatattgcaa ttttctgtag
      181 attctgttga gcatcaaaca acccaacaac tttcaactga ggaccaaatc ctgacgcagg
      241 gtttcctgta accggactgg gaaagcgata aaaacctctt gaatcgacat attccgaaga
      301 taacctgagt tcttccactg taatatcacg ccagccttca ttaagaacat cggtagaata
      361 atcaatccta tctgggtgct ttgcaaatag cctggatacc agaaaacttc ctaatccaag
      421 aacctttcct gtattggcat atactgcaat attataagac gcagagatca ggcctgttat
      481 ttgcgcttga tgataatctt tataattaaa gagtcccatg agtatttcct tatttaaaga
      541 acagccccat gctggggctg tttgtttaga ttttctttta tttaacctca ataccctcct
      601 gtatccaaag agttaaacca ggaatatcag tggactgata ggtgaaatat ttaacatcac
      661 catcattggc actacctaaa ttctcccagt cgaagttttg tacatccacg gatgcacctg
      721 aactacctga gatttttaat atctccccct ctatatcctg agatttaaaa tcatctgaat
      781 tcagaatgag atgagatgta gagtgcaaat caacatgctc aaaaccagag aacatagcac
      841 ttgttactgt agtattgtcc aaagatatcg tgtcaaagcc ctctccacct ataatattta
      901 cctgagtacc attgttagtt gaaatctctc cattagcgta aatgaagtca ttacctgccc
      961 ccaagtctat attcgtatta ataatagcat ccgcactatt accttcatac cctaaagcca
     1021 gaacagtatc atttcctgcc cccataacaa tatcagttcc tgatattgtt ccacctgatg
     1081 atgaagaatt gagcccagta tagtcaatgg atgtactacc attataacta tcagtagttg
     1141 ctctcagttg tccttcttca ttcacatata gtgttacatc gttatttccg acaccaacaa
     1201 ccaaggtatc atctccattc cccatatcag tacgatgaga gtgatccgtg tatcctgcac
     1261 tggtattacg tccataatct cttcctgtaa cgattaaatc attcccttct cctgtgaata
     1321 aaccaaccgg gaagcgaaat acttgagttc tatctaatcc tcctaaagaa tcggtaattg
     1381 agataacatc agaacctgta tgaccattgt aattatattg attagctcct gaatctaatc
     1441 tctgggatgt aacataactt ccaggattac tatcaagtaa agtagaccag ttcttagaaa
     1501 cagaagatac cttatcacca aatatggacg gttgaggata taagtctcct gatacattag
     1561 agggcaatgc ccgttcgtaa aatgcc
//
LOCUS       DAZGAP010000403         1572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGAP010000403 DAZGAP010000000
VERSION     DAZGAP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.405"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..1481)
                     /locus_tag="ACHLAM_005332"
     CDS             complement(57..1481)
                     /locus_tag="ACHLAM_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFO7856683.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIVSSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
BASE COUNT          528 a          424 c          263 g          357 t
ORIGIN      
        1 cggtaagatg ctcaacatta ccgggcatta ctgctgattg tgacacaatc agactattac
       61 atcatagctt taccctgaat ttcacgcagt ttatttccgg caagctgctc aatttgtgtt
      121 agcgggatcc cctttgtttc aggaacatag cgagaaataa aaacataaca aaccagatta
      181 aagacaacaa aaatccacat agaaaacgct ccgccaaagg tttcctgcag ccaggtatta
      241 tcatttatta ccggaaacaa cagtgatatt agaaaattcg caatccacat taaactaacc
      301 gccagcccca tgccgaatcc tttaatcttt tcgggaaaca cctcagaaat cagtacccat
      361 gctcctacgc tccagctgac agcaaacaac agcataaaaa ataaaatccc aaaaatggtg
      421 atatagccag tatcatgggt atacaaacca tatgatgcga tcaacaaacc aataattgac
      481 ccgatagtgc caatcttcat gattggtatt cgtccatagc gatcgaacaa aatcatcccg
      541 ataaaactac cgatcacatt acaaacagca ataaaaatcg tctggaacat cacttcctct
      601 gtactgctgt tttgtagcac cagaggtgcg tagtagttca tcacattgac accagtaaac
      661 tgttgtaaaa cagcgataca acagccaacc agcagaatat agcgcaacac aggagagctt
      721 ttcaggataa cgaattccct gcctgcagca ttctcccggg cctccgtttc cctcatacta
      781 tcaaaaatag cggctacatc ggtagcgcta accccccggt agattctggc aagcactttc
      841 agagaacgtt tttccccctt gttattacgg gcacaccatg ccggcgattc aggcaaaaat
      901 aaagttatca ctaacattgt aacagaaggt atcacctgag cgaataacat gctcttccag
      961 ccttcttcga ccagcacatc agagggcatc cccttagcaa tcacataatt aacaataaaa
     1021 acaatcacct gaccagaaac tactgatata ttgtaaatac ccagagcttt accacgaatg
     1081 tcagctggtg ccagttcaga catatacatg gtagatgcag tccccgccat cccaacagca
     1141 aaaccactga cgatgcgcga aatcgagaaa accataaaag attcactcat cgccccccac
     1201 agtgacgaga cgataaaaat aactgcaata atcagaagcg caggcttacg ccccaactta
     1261 tcggcaattt tccccccgac cagcgcacca atgatacaac caatcacaat gctcgatact
     1321 gcccacccag tttcagttga agtgagctgg taatattgct gtaaaggcag tatcaccccc
     1381 gttatcactg ccgtcgaata gccaaacatc aatccagcca gtgcagcaac gcagcacaat
     1441 gcgtacacat aggctgaatc atacttgctt ctctgaaaca taccccctcc ctgcctgttg
     1501 aggaaatgac atacgaacat ttattccata cttaaacaaa tcagctcaaa tattccacaa
     1561 caaagatcac aa
//
LOCUS       DAZGAP010000404         1572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGAP010000404 DAZGAP010000000
VERSION     DAZGAP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.406"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(223..>1572)
                     /locus_tag="ACHLAM_005333"
     CDS             complement(223..>1572)
                     /locus_tag="ACHLAM_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856684.1"
                     /translation="GGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIG
                     FENDPVFRVLGDGAGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPF
                     SVLALPLGSWSAHFAGSKTDAFERILGAQFYDYMSPDSTVIVDEQSSWTSWYSWVHDK
                     GDESRERPVFIIGNDGNNLLAGGKGGDYIEGGGGNDKIEPGKGANRVDGGSGYDTLLL
                     SDVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITD
                     HRYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGN
                     DYLHGASGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHK
                     GEKDFLFLSKDIVTSTDEFISCLKQTGNDVEAGIAGGRIYFVNTALEDINHNSVYIV"
BASE COUNT          497 a          429 c          238 g          408 t
ORIGIN      
        1 ttaatatata atcaggagag aagtgaaaga gtacccggtg cgaaaatgaa ttaatctgag
       61 cagatttaac ggtaaacgaa gcgcaataaa ttaaaattaa aaaacaaaat cacttgcagt
      121 ctattatctt tacgttcctc accgcaaccg cacacaaaaa caaaacacac aatgcattga
      181 ttttaaacaa aaaaaacaaa acagaaactc aacacataaa aatcagacaa tataaacaga
      241 attgtgattt atatcctcaa gcgctgtatt aacgaaatat atccgtcctc ctgcaatacc
      301 tgcctcaaca tcatttccgg tttgtttcag acatgaaatg aactcatctg tcgatgtaac
      361 aatgtccttt gataaaaaca gaaaatcttt ttcaccctta tgtttattaa aatccatgat
      421 aaatgaattc ccttcacaag cagcatcaaa gacgaagaca tcatgaccag taccaccata
      481 cagataatca ttacctcccc caccatataa ccagtcgtta ccttctgctc cgtacaactc
      541 atcattacca gaagccccat gaagataatc attcccgcga cctccgtgta aaagacttac
      601 tccttctccc ccggcactat gtaatatatc atttccgtca agaccaaaaa taatatttcc
      661 gaacagacta tcatctccgt ttgtaccttc tgtatgtttt ccgtaatcaa catttttatt
      721 acgccacttc agaagatacc tgtggtctgt aatctgctct tcttctatct gataagggct
      781 tagtttactc ctccagtcac cagaaaaaga gataccttca atacctgttg cctcagtcag
      841 accggctccc cgttctgaat ggaaaaacag cgtcccatca ctgaccctgt atatattcca
      901 gtcatccatt acatcgctaa gaagcagcgt gtcataccca ctgccaccat caaccctgtt
      961 tgctccctta cctggttcaa tcttatcatt ccctccccct ccctctatat agtcgccacc
     1021 cttcccgcct gccagcagat tattgccatc attaccgatg atgaatacag gtctttcacg
     1081 actctcatcc cccttgtcat gaacccatga ataccaagat gtccagctac tttgctcatc
     1141 cacaatgact gtactgtccg gtgacatgta gtcgtaaaac tgagccccta aaattctttc
     1201 aaaggcgtca gtttttgaac ccgcaaaatg cgcactccag cttcccagcg ggagtgcaag
     1261 cacagaaaag gggataatat tccagaggaa tgaaccataa aggtctgtgt ataaaacaat
     1321 gttatcagga gaagagtcat attcacgatc cggggttgtc aagcccggcc ccattgcttt
     1381 aactgcctcc cagaacccgg ctccatctcc cagcacacga aataccggat cgttttcaaa
     1441 accgatattt aataccttcc catccccgtc aaataccagc ggggaagccc aggctatata
     1501 gtcagaatta gcataaaaac catcagccag agactcactg agtctggcca taatgttagt
     1561 caacccaccc cc
//
LOCUS       DAZGAP010000405         1568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGAP010000405 DAZGAP010000000
VERSION     DAZGAP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.407"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..280
                     /locus_tag="ACHLAM_005334"
     CDS             <1..280
                     /locus_tag="ACHLAM_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856685.1"
                     /translation="RMKTNIPYKTNDDMKKSTGGIITGSKFIGLIIVDLEEEKFFVYQ
                     YSLNNRIKRKFRAYMKIKKDSPVKAITQFTKVNIWYWDEMLKQLMNKL"
     gene            373..750
                     /locus_tag="ACHLAM_005335"
     CDS             373..750
                     /locus_tag="ACHLAM_005335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856686.1"
                     /translation="MIKTLFDPETMSREDMLREIQLLRDSRDLALELVFGYPETKGAE
                     QIELNDDEITLLKAKLPEHHFQWLAGDGDEALTVGRLGYFADLADDFSVLAGEIDPDL
                     EEPEEEVISRGPCGVVFCLIESD"
     gene            1020..1442
                     /locus_tag="ACHLAM_005336"
     CDS             1020..1442
                     /locus_tag="ACHLAM_005336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856687.1"
                     /translation="MGDWHTKPRTGASQFAEYEEEIRLLKEKQYTNKQIYEAISQKGI
                     TIGQQMFNIYMRRMFNGETYIKTDVLKNKKEKTNLNNNIHKGNTAWNNVNIRSVRLIK
                     DLESHGFTPEIVREWKEPNEMSVRQHLTVLISKKGIIK"
     gene            1439..>1568
                     /locus_tag="ACHLAM_005337"
     CDS             1439..>1568
                     /locus_tag="ACHLAM_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7856688.1"
                     /translation="MILKIVVLNNSGNVGKSTLCRSLLSPRIAGSKIINIESINTDG"
BASE COUNT          583 a          227 c          347 g          411 t
ORIGIN      
        1 cagaatgaaa acaaatatcc catataaaac taatgatgat atgaagaagt ctaccggagg
       61 gataattaca ggtagtaaat ttataggact aatcattgtt gatctggaag aagaaaaatt
      121 ttttgtgtat caatatagcc tgaataacag aatcaaaaga aaattcaggg catatatgaa
      181 aataaaaaaa gacagtcctg taaaagcaat aactcaattt acaaaggtaa atatatggta
      241 ttgggatgaa atgttaaagc aacttatgaa taaattatga cagacaaggc ggggaaaccc
      301 gccttactct gtattattac ttgtgttagc atagttaaaa acaagcatta ataaattaaa
      361 cgagatatat ttatgattaa aaccttgttc gatccagaaa ccatgagcag ggaggatatg
      421 cttagggaaa ttcagctact gcgcgactca cgtgatctgg cgcttgaact ggtgttcggc
      481 tatccagaaa caaagggcgc agaacaaatt gaattgaatg atgatgaaat aacccttcta
      541 aaagcgaaac taccggaaca ccattttcaa tggttagcag gagatggaga tgaggcgcta
      601 acagttgggc gattaggtta ttttgcagat ctggccgacg attttagcgt tctggccgga
      661 gagatagatc cagacctgga agaaccagag gaagaagtga tatcccgtgg gccttgtggt
      721 gtcgtatttt gtttgattga gtctgactga tcttgtgtgt gaggtgtagt aaatgccgcg
      781 tctgcggcat gggaggcgac aaaaacaagt taaatataaa ttggatgtaa agtgtcgccg
      841 tgacgcggct acgccgctcg gatgtaaata aatcacagga acacctcccc accctgccag
      901 acgtgtttgt gtctggcgca gaatgcgcca gacaaccata tatcaggcaa cacgttactt
      961 aatggtcgta gacaagcaat tgtaacagta acaggcttga attttatgga gatgatgaaa
     1021 tgggagattg gcatactaag ccaagaacag gagcttctca atttgctgag tatgaagagg
     1081 aaataagatt attaaaggaa aaacaatata cgaacaaaca aatatatgag gcaatatccc
     1141 agaaagggat aacaatagga cagcaaatgt ttaatatata tatgaggcgt atgtttaatg
     1201 gcgaaacata tataaaaaca gatgtattaa aaaataaaaa agaaaaaaca aatttgaata
     1261 ataatataca caagggtaat acggcgtgga ataatgttaa tataaggagt gtgcgattaa
     1321 taaaagatct tgagtctcac ggttttacac ctgaaatagt tcgagagtgg aaagaaccaa
     1381 atgagatgtc tgtaagacaa cacttgacag tattaattag taaaaaggga ataataaaat
     1441 gattctaaaa attgttgtat tgaataactc tggtaatgtt ggcaagtcta ctttgtgtag
     1501 atctttatta tcgccaagaa ttgcaggttc aaaaattatt aatattgaaa gtattaatac
     1561 agatggtt
//
LOCUS       DAZGAP010000406         1521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGAP010000406 DAZGAP010000000
VERSION     DAZGAP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.408"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1513)
                     /locus_tag="ACHLAM_005338"
     CDS             complement(<1..1513)
                     /locus_tag="ACHLAM_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7856689.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLVDREPGPVMVFNGEKNVPADSLKSQRLIYPDSNNHTGK"
BASE COUNT          422 a          372 c          368 g          359 t
ORIGIN      
        1 acttacctgt atgattatta ctgtcaggat aaataagtct ctgtgactta aggctgtctg
       61 caggaacatt tttctcgcca ttaaacacca taaccggccc cggctctctg tccacgagcg
      121 ggttgaatgg cggtatattt tcagtacgaa tgccggtcag ccactgaaaa atacctgcga
      181 aatgacgggc actgattttc ctgctgacgg actgatgaga cgtgatgtca ctggcggtaa
      241 taatcagggg aacgctgtag cctccctgca catgaccatc atgatgaaca ggattggcac
      301 tatcgctgac cgacagccca tggtcagaaa agtaaagcat ggcaaaatga cgggaatgcc
      361 ggcgaaggat accatcaagc tgaccgagaa agttatccag tttactgatg ctggcgaggt
      421 aacaggcaat ctttcgggga tactgctcca ggtaatgatt cggccaggag ttaagtcggt
      481 cacacggatt cggatgagag ccaatcatgt gcaggaaaat caccttcgga gaggatttat
      541 ccgccagcgc acgttctgtt tcctgtaaca acaacatgtc atccgtttta cgggaagcga
      601 agctgccttt cttgaggaaa acggtatgct ccgcatcaga agcaataaca gagatgcgtg
      661 tgtcatgctc ccccagtttt ccctgattgg atatccacca tgtgctgtat cctgcttttg
      721 ctgccagcgc caccacgttg ttgccggagt cagggttctg ctcatagtca taaatcagtg
      781 tccggctcag ggaaggtacg gtactggctg ctgccgatgt atagccgtca ataaataaac
      841 cgggagccgt attcagccac ggtgtggttg gtacgggata accatatacc gacatataat
      901 ccctgcgcac actctcacca gtgacaatca caatcgtgtc atacaacggt gctcccggca
      961 ggattttcca gctgtcagcc ctgtgctgac tcagttgttc ataacgctgc atttcacgca
     1021 atgtgtcagt tgtccccaca acggttcctt taaccatccg caacggccag ctgtttacag
     1081 agcataatac gaacagcagc agtgccagcc agttacggtg accgcggtgg tgtgtccgcc
     1141 agaaaataac catgaacacc agaatcgcag cactgaccag aaaatgataa acaggaatca
     1201 tcccggtaaa ctccgcagcc tcatctgccg tggtctgcaa caacgcgaca ataaaactgt
     1261 tgttgatttt accgtacgtc atacccgcag gcgcatacag tgcacaacag aacagaaata
     1321 acagagctgt aatggatgta agggtatttc tgtgtgcaag gagcagaaga aggaacagca
     1381 gcaacacatt cccggtggca ttcttctcag tgtatccgca tgcaattgtg gttatgacag
     1441 aaataacaaa aaagaataaa aacaataaaa tccgtggtgt gttgtccgga caaaacagtt
     1501 ttctgatatt catcggagta t
//
LOCUS       DAZGAP010000407         1520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGAP010000407 DAZGAP010000000
VERSION     DAZGAP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.409"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..915
                     /gene="tssG"
                     /locus_tag="ACHLAM_005339"
     CDS             <1..915
                     /gene="tssG"
                     /locus_tag="ACHLAM_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7856690.1"
                     /translation="PWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            951..1475
                     /gene="tssJ"
                     /locus_tag="ACHLAM_005340"
     CDS             951..1475
                     /gene="tssJ"
                     /locus_tag="ACHLAM_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7856691.1"
                     /translation="MVTVIRWWVPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDVAGYQMLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
BASE COUNT          313 a          429 c          458 g          320 t
ORIGIN      
        1 ccctggcccg ggatgggatt cccggtcagc accctgaaag cggtggagat cgacgaagac
       61 cgtccgacat tacccccaac aatccgcacg acatttctcg gaatgtatgg ggtggactca
      121 cctctgccga cgtcctggct ggatgatatt acgcagcgcc gtgaaggaca tgaggcactc
      181 acctcatttc tggatatttt cagccaccgc atcaccacgc agtactaccg gatctggcga
      241 aaatatgcgt acccggccac ttttgaggag ggggggcgcg atgccacatc gcagtgtctg
      301 ctggggctgg tcgggctcgg tatacccggc accgctgaac aggtggcaac gccggtttcc
      361 cgtttcctgg ccttactggg tgccatgcga ttacccaccc gcaatgctga aggcatccgg
      421 gctctggtga gtctgctggc accggatacc cgcgcactta tcaccgaacc cgatccggtt
      481 aaggtccata tcggtaaccg cagtggactg ggtgccggaa accgcatccg tctttcgcag
      541 cgggcgccgc tgggtaaaac gacaggggaa gcatgcagcc ggctcctgat gacactggaa
      601 acagcagatc cgaacgaagc ggaaggctgg ttgcccggtg gtgtcctcca taccgatttg
      661 ctggtcctgc tgcgtgttta tctcggctac cgcagtgacg tccggctgcg actcactgtc
      721 cccgtgcggt tgttgcctga gccccgcctg ggcaaaggca ggcctgttca gctgggccgt
      781 accggtttgc tggggctgaa agcgggcaag ctcagcaatg gccgggagag tctgacggtc
      841 agtctgggct gctacgaagg gttgaaatgt gctgcgctac cgcctgctga agacggacat
      901 tatcgcttcg aataatcacc tgttctctct ctcaataaag gagcgcctga atggtaacag
      961 taatccgctg gtgggtgccg atgtttattg cctgtggcct gctcgcaggc tgcggtctga
     1021 cgcagaccgt cacggacggc acgatcagca tcacgaaatc catcttctac aaaaaaatca
     1081 aaacgctgca tctggatttt accccacgca cggccattaa cactgacggg gcacagacgc
     1141 cactggcaac catggtgcgg gtttaccagc tcagggatcg taaagccgtt gatgtcgccg
     1201 gttaccagat gctgctgcgt aatgccgata cggtacttaa ggacgacgtg ctggcctcaa
     1261 aagagctgct ggtgatgccc ggcggcagcg tgacgctcaa tatgcccatg gatgaagatg
     1321 cgcagtttgt ggctgtggtg ggattgttta accggccgga tcagaaggac aatcgctggc
     1381 gcctggtact gacccgtgat gacctcaatc cggataagcc ccgcaccatt gaactgggtg
     1441 atggctggct cagccttgtg ccggtaaagg agtgacgtga tggataaccg gtcagcgttt
     1501 tttgacgggc agacccgtta
//
LOCUS       DAZGAP010000408         1519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGAP010000408 DAZGAP010000000
VERSION     DAZGAP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.410"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            397..1324
                     /locus_tag="ACHLAM_005341"
                     /pseudo
     CDS             397..1324
                     /locus_tag="ACHLAM_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1370..>1519)
                     /gene="rpmE"
                     /locus_tag="ACHLAM_005342"
     CDS             complement(1370..>1519)
                     /gene="rpmE"
                     /locus_tag="ACHLAM_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO7856692.1"
                     /translation="GVTYPYVTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVG
                     TQKGE"
BASE COUNT          437 a          350 c          375 g          357 t
ORIGIN      
        1 agcagttgca ctaatgggtg gggggatagc atacttacca tatcctcttg ttcaatcaga
       61 aatactgtca ggaaaactag tatcaataat gaatgattat attaaaactc gttttgaagt
      121 aagtctgtgg tttaggaagc ccctatcctt gcgttgtgtt acatttagga atttcctagt
      181 agaaagagcc tcacaactca aggatactgt acaattcaga taaatcactt aacttgatgc
      241 atcaatcacc cagcgaccag ttatgctcat cataatcatt attcaggtgt gagcataatg
      301 agcataaccc agcgatccag acaggttccc tgaatctcca gacaaccaat atcaattaaa
      361 taagtgatag tctaaaaact agtcattaga gaacagatga ttgatgtctt agggccggag
      421 aaacgcggac ggcgtaccac acaggaaaag atcgctattg ttcagcagaa ctttgaaccg
      481 ggaataactg tctccctcgt tgcacggcaa cacggtgtga cagccagtta tttctctggc
      541 gtaagcaata ccaggaagga aatctcactg ctgtggctgc tggagaacag gttgttctgg
      601 cctctgagct tgctgccgcc atgaagcaca ttaaagaact ccagcgcctg cccggcaaga
      661 aaacgatgga aaattaactc attagagaag ccgttgaata ttggcgtgca aaaaagtgga
      721 tagcgcacgc ccaatattgc ccgggaatgg ggagtaagtt tcgtcagccg ttatctccgt
      781 gtgttgcgtg cgcagttgca cgtcattctc agacgaaccg atgactggaa ggatggccgc
      841 cgcatgatac ggatgtttat ctccgtaata acatggtgta caccatgtta tcggagagct
      901 gcccacgtat ggttatcgtc ggctgtgaaa cagtacagga cgtcatgctg ggagcggtag
      961 aacaccgctt cggcagcgac cttcctgcgt ctccagtgga gtgcctgacg gataatagtt
     1021 catgctatca ggctaatgaa acacgccagt tcgcccggat gttggaactt gaaccgaaga
     1081 acacggcggt gcggtatccg gagagtaacg gcatagcaga gagcttcgtg aaaacgataa
     1141 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
     1201 cagaggcgtt cgagcattat aacgaatggc atccacatag tgcgctgggt tatcgctctc
     1261 cacgggaata tctgcggcgg cgggcagtaa tgggttaagt gataccagat gtctggaaat
     1321 ataggagcaa atccaccacg catggagcct gttaagactc tcactctcat cattctccct
     1381 tctgagtccc aacgaaacga ccaaaacgct gagtaaagcg ggcaacattg ccttctgatg
     1441 ccaccgttct gagtttaccg gtatagaacg gatgcgattt ggaggaaacg tcaatcgtca
     1501 catacggata cgtcacacc
//
LOCUS       DAZGAP010000409         1518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGAP010000409 DAZGAP010000000
VERSION     DAZGAP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.411"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..915
                     /gene="tssG"
                     /locus_tag="ACHLAM_005343"
     CDS             <1..915
                     /gene="tssG"
                     /locus_tag="ACHLAM_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7856693.1"
                     /translation="PWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIDNRSGLGAGKRIRLSQRAPLGKTTGEACSRLLMTLETADPDEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPSAEDGHYRFE"
     gene            949..1473
                     /gene="tssJ"
                     /locus_tag="ACHLAM_005344"
     CDS             949..1473
                     /gene="tssJ"
                     /locus_tag="ACHLAM_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7856694.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGMISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDAADYQMLLRRADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
BASE COUNT          315 a          418 c          459 g          326 t
ORIGIN      
        1 ccctggcccg ggatgggatt ccctgtcagc accctgaaag cggtggagat agacgaagac
       61 cgtccgacat tacccccaac aatccgcacg acatttctcg gaatgtatgg ggtggactca
      121 cctctgccga cgtcctggct ggatgatatt acgcagcgtc gtgaaggaca tgaggcgctc
      181 acctcatttc tggatatttt cagccaccgc atcaccacgc agtactaccg gatctggcga
      241 aaatatgcgt atccggccac ttttgaggag gggggacgcg atgccacatc gcagtgtctg
      301 ctggggctgg tcggactcgg tatacccggc accgctgaac aggtggcaac gccggtttcc
      361 cgttttctgg ccttactggg tgccatgcga ttacccaccc gcaatgcgga aggcatccgg
      421 gctctggtga gtctgctggc accggatacc cgcgcactta tcactgaacc cgatccggtt
      481 aaggtccata tcgataatcg cagtgggctg ggtgccggaa aacgcatccg tctttcgcag
      541 cgggcgccgc tgggtaaaac gacaggggaa gcatgcagcc ggctcctgat gacactggaa
      601 acagcagatc cggacgaggc ggaaggctgg ttgcccggag gtgttctcca taccgatttg
      661 ctggtcctgc tgcgcgttta tctcggctac cgcagtgacg tccggctgcg ccttactgtc
      721 cccgtgcggt tgttgcctga gccccgcctg ggcaaaggca ggcctgttca gctgggccgt
      781 accggtttac tggggctgaa agcgggcaaa ctcagcaatg gccgggagag tctgacggtc
      841 agtctgggtt gctacgaagg gctgaaatgt gctgcgctac cgtctgctga agacgggcat
      901 tatcgcttcg aataatcacc tgttctctct caataaagga acgcctgaat ggtaacagta
      961 atccgctggt gggcgccgat gtttattgcc tgtggcctgc tcgcaggctg cggtctgacg
     1021 cagaccgtca cggatggtat gatcagcatc acgaaatcca tcttctacaa aaaaatcaaa
     1081 acgctgcatc tggattttac cccgcgtacg gccattaaca ctgatggggc acagacgcca
     1141 ctggcaacca tggtgcgggt ttatcagctc agggatcgta aagcggttga tgccgccgat
     1201 taccagatgc tgctgcgccg ggccgatacg gtacttaagg acgacgtgct ggcctcaaaa
     1261 gaactgctgg tgatgcccgg cggcagcgtg acgctcaata tgcccatgga tgaagatgcg
     1321 cagtttgtgg ctgtggtggg actgtttaac cggccggatc agaaggacaa tcgctggcgc
     1381 ctggtactga cccgtgatga cctcaatccg gataagcccc gcaccattga actgggtgat
     1441 ggctggctca gtcttgtgcc ggtaaaggag tgacgtgatg gagaaccggt cagcgttttt
     1501 tgacggacag ccccgtta
//
LOCUS       DAZGAP010000410         1507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGAP010000410 DAZGAP010000000
VERSION     DAZGAP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.412"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..1466
                     /gene="gndA"
                     /locus_tag="ACHLAM_005345"
     CDS             60..1466
                     /gene="gndA"
                     /locus_tag="ACHLAM_005345"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO7856695.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIV
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          384 a          360 c          381 g          382 t
ORIGIN      
        1 taaacttttt aagttattct cataccgcac atgcggtaaa accctgacag gagtaaacaa
       61 tgtcaaagca acagatcggc gtcgtcggta tggcagtgat ggggcgcaac ctcgcgctca
      121 acatcgaaag tcgtggttat accgtctcta ttttcaaccg ttcccgtgaa aagacggaag
      181 aagtgattgt cgaaaatcca ggcaagaaac tggttcctta ctatacggtg aaagagtttg
      241 ttgaatctct ggaaacgcct cgtcgcatcc tgttaatggt gaaagcaggt gcaggcacgg
      301 atgctgctat tgattccctc aagccatacc tcgataaagg tgacatcatc attgatggtg
      361 gtaatacctt cttccaggac accattcgtc gtaaccgtga gctttctgcc gaaggcttta
      421 attttatcgg taccggtgtt tccggtggtg aagaaggtgc gctgaaaggt ccttccatta
      481 tgcctggtgg gcagaaagaa gcctatgaac tggttgcacc gatcctgacc aaaatcgccg
      541 cagtggctga agacggtgag ccatgcgtta cctatattgg tgccgatggt gcaggtcact
      601 atgtgaagat ggttcacaac ggtattgaat acggcgatat gcagctgatt gctgaagcct
      661 attctctgct taaaggtggc ctgaatctca ccaacgaaga actggcgcag acgtttaccg
      721 agtggaataa cggtgaacta agcagctacc tgatcgacat caccaaagat atcttcacca
      781 aaaaagatga agacggtaac tacctggttg atgtgattct ggatgaagcg gctaacaaag
      841 gtaccggtaa atggaccagc cagagtgcgc tggatctcgg cgaaccgctg tcgctgatta
      901 ccgagtctgt gtttgcacgt tatatctctt ctctgaaaga tcagcgcgtt gccgcatcta
      961 aagttctctc tggtccgcaa gctcagtcag caggcgacaa ggctgagttc atcgaaaaag
     1021 ttcgtcgtgc gctgtatctg ggcaaaatcg tttcttacgc tcagggcttc tctcagctac
     1081 gcgcagcgtc tgaagagtac aactgggatc tgaactatgg cgaaatcgcg aagattttcc
     1141 gtgctggctg cattatccgt gcgcagttcc tgcagaaaat caccgatgct tatgccgaaa
     1201 atccgcagat cgctaacctg ctgctggctc cttacttcaa gcaaattgcc gatgactacc
     1261 agcaggcgct gcgcgatgtc gttgcttatg cagtacagaa cggtatcccg gttccgacct
     1321 tcgccgctgc ggttgcctat tatgacagct accgtgccgc tgttctgcct gcgaacctga
     1381 tccaggcaca gcgtgactat ttcggtgcgc atacttataa gcgcattgat aaagaaggtg
     1441 tgttccatac cgaatggctg gattaatctg atttaatcaa caataaaatt gaggcccggc
     1501 gtatatt
//
LOCUS       DAZGAP010000411         1501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGAP010000411 DAZGAP010000000
VERSION     DAZGAP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.413"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..481
                     /gene="curA"
                     /locus_tag="ACHLAM_005346"
     CDS             <1..481
                     /gene="curA"
                     /locus_tag="ACHLAM_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO7856696.1"
                     /translation="CRHATEVLGFDVCLDHHADDFAEQLAKACPKGIDIYYENVGGKV
                     FDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDY
                     GHRIQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNFGKVVIRVAVDD"
     gene            679..1344
                     /gene="mcbR"
                     /locus_tag="ACHLAM_005347"
     CDS             679..1344
                     /gene="mcbR"
                     /locus_tag="ACHLAM_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO7856697.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(1380..>1501)
                     /locus_tag="ACHLAM_005348"
     CDS             complement(1380..>1501)
                     /locus_tag="ACHLAM_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856698.1"
                     /translation="LFDKEYVGSVIVNESNGRYYEPAPGRNYGVGMNIAWRFE"
BASE COUNT          444 a          318 c          358 g          381 t
ORIGIN      
        1 atgccgccat gctactgagg tgctgggctt cgatgtttgt cttgaccatc acgcggatga
       61 ttttgccgaa caactggcga aagcgtgccc aaaaggtatt gatatctatt atgaaaacgt
      121 gggcggtaag gtatttgatg cagtgctacc tttacttaat acatctgcgc gcattcccgt
      181 ttgtggctta gtgagcagct ataacgctac agagctacca cccggtccgg atcgtttacc
      241 tctgctgatg gctacggtgc tgaaaaaacg tattcgcttg caaggattta ttatcgctca
      301 ggattatggt caccgcatcc aagagtttca gaaggagatg gggcaatggg tgaaagagga
      361 taaaatccac taccgcgaag aaattactga cggtttagag aatgcgccac agacgtttat
      421 cggcctgctg aagggtaaaa acttcggcaa agtggtgatc cgcgtggcgg ttgatgatta
      481 aagtttcaac ggcggcgtaa gccgccataa aaatccaggc aaaaagctta ttttcagctt
      541 taattaacta accacgtcat tgttctgctg ctaataacaa caaaggtgag tggtttatga
      601 ttattcactt taatacacca ggtgaactcc ttctgccatg caggcagggt tggacagaaa
      661 acacgcgaag gaattaaaat gcctggaacg gaaaaaatga aacatgtcag tttgactctg
      721 caggttgaga acgacctgaa acatcagctt agtattggcg cattaaaacc tggcgcacgc
      781 ctgattacta aaaatctggc ggagcaatta ggtatgagta ttacacctgt gcgtgaagca
      841 ttattacgtc tggtttcggt gaatgcgctt tctgtcgcac cagcgcaagc atttacagtt
      901 ccggaagtgg ggaaacgtca attagacgaa atcaatcgga tccgctacga gctggaatta
      961 atggcagtta ctctggctgt tgaaaacctc accccgcaag acctggcgga actcgaggaa
     1021 ttgctcgaga aattacagca ggcgcaagaa aagggcgaca tggaacaaat cattaatgcg
     1081 aacaggctat ttcgcttagc gatttatcat cgttcaaata tgcccatcct gtgtgagatg
     1141 attgagcaac tgtgggtcag gatgggacct ggtttacatt atctctatga agcgattaat
     1201 ccagcggaat tacgggagcg tatagaaaac tatcgtctat tactcgccgc gttaaaagca
     1261 aaagacaaag agggatgcag acattgtctt gctgaaatta tgcaacaaaa tattgctatt
     1321 ttataccagc aatacaatcg ttaaattaaa tgcccggcaa ataccgggca tcaaggtaat
     1381 tactcaaatc gccacgcaat attcatgccg acaccataat ttcgcccggg cgcaggttcg
     1441 taatatcgcc cgtttgactc attgacaatg acagagccaa cgtattcttt atcgaataaa
     1501 t
//
LOCUS       DAZGAP010000412         1464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGAP010000412 DAZGAP010000000
VERSION     DAZGAP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.415"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..394
                     /locus_tag="ACHLAM_005349"
     CDS             <1..394
                     /locus_tag="ACHLAM_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7856699.1"
                     /translation="NITQAVAPIMMEQNHGAIITMSSVVGTDGNIGQSNYAATKGGVI
                     AMSKGWAKEFSRKGAKVRVNCVAPGFVITPMTENLPEKVINHMIAKTPLGRMATPEDI
                     SNGVLFLASEKSSFITGQTLKIDGGLVI"
     gene            404..>1464
                     /locus_tag="ACHLAM_005350"
     CDS             404..>1464
                     /locus_tag="ACHLAM_005350"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO7856700.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFAFESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILASEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVLKSISEN
                     HSVSIDWLNQRTNVNGGAIA"
BASE COUNT          476 a          224 c          349 g          415 t
ORIGIN      
        1 taatataact caggcagttg cacctattat gatggaacaa aatcatgggg caatcataac
       61 aatgtcttct gttgtaggaa ctgatggtaa tataggtcag agtaattatg ccgcaactaa
      121 aggtggtgtt attgcaatgt ctaaaggttg ggctaaagaa ttttcccgaa aaggtgctaa
      181 agtacgcgtg aattgtgtag ctccaggatt cgttattacc ccgatgacag aaaatctgcc
      241 agagaaagtc attaaccata tgatagctaa aaccccttta gggcgaatgg caacacctga
      301 ggatatttcg aatggtgttc tttttttagc tagtgagaaa tcatctttta ttactggtca
      361 aacattaaaa attgatggtg gattagttat ttaaggagga ggaatggaaa aagtatacat
      421 tacaggggca aaaagaacgc ctataggatg ttttggcggt tcgttatcaa cagtgtcagc
      481 ttcagatctt ggagcaattg cgatatctgc agcaattaaa cagagcagaa taaacgttga
      541 atgtattgat gaagttattg tcgggaatgt tttgtctgct ggacaaggaa tggggccagg
      601 tagacaagct gctgttaaag ccggtatacc atggaaaact ccggctctga ctttgaatat
      661 gatttgtggt agtggtatga aaactattat cgatgctgca atgaaaattc gtagtggaga
      721 tatggatgtt gttgttacgg caggcataga gaatatgagt caggcacctt acttgattga
      781 tcatcatgca agaagtggtg tacgaatggg taatcaggta atgagtgact ctcttattac
      841 agatggtctt actgatgctt ttaatcacta tcatatgggg attacagcag aaaatatagc
      901 ccgacagttc aatattacaa gatatgaaca ggatacattt gcgtttgaaa gtcagaagag
      961 agccttaaag gccattgaca atggagaatt tgaggatgaa attactccgg tacaagtaca
     1021 tacccgaaaa ggaaatattg aagttactac agatgagtat cctcgtagag actcctcatt
     1081 agagaaactt tctgtgttac gtccagcatt tgaaaaaaat ggtaccgtga ctgcaggaaa
     1141 ttcatctgga ataaacgatg gtgcagcagc tcttattctt gcttcagaga aagcaattca
     1201 gacattggaa ttacaacctt tggctgaaat tcgctcattt ggacaaggag gcgttgatgc
     1261 atcagtgatg gggctggggc ctgttccagc aatagataat gcattaaaaa aagcaaaact
     1321 ggatattaaa gatattgatc tttttgaaat taacgaggcg tttgcggctc aggcaatagg
     1381 ggtcttaaaa agtatttctg aaaatcactc agttagtatt gattggctaa atcaaagaac
     1441 aaatgttaat ggaggagcga tagc
//
LOCUS       DAZGAP010000413         1462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGAP010000413 DAZGAP010000000
VERSION     DAZGAP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.416"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..460
                     /gene="rfaY"
                     /locus_tag="ACHLAM_005351"
     CDS             <1..460
                     /gene="rfaY"
                     /locus_tag="ACHLAM_005351"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO7856701.1"
                     /translation="GDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLI
                     EYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSG
                     KSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
     gene            486..>1462
                     /locus_tag="ACHLAM_005352"
     CDS             486..>1462
                     /locus_tag="ACHLAM_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO7856702.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVKIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFI"
BASE COUNT          446 a          257 c          307 g          452 t
ORIGIN      
        1 aggtgactac tatcagaatt tgatcgttga aaccgatcgt gtcagaagtg caggtcttac
       61 ttttcctaat gatttttatt ttctggctga gcgtaaaatt ttcaactatg ccagtgtttt
      121 tatcatgctt atcgagtatg tcgagggcgt agaactcaat gatatgccga ttattccaga
      181 aaatgttaaa gcagaaatta aggcttcaat ggaaaaattg catgccttaa atatgctatc
      241 gggcgaccct caccggggta actttatcgt tagtaaagat ggtgtcagga taattgacct
      301 atccggtaaa agctgtacgg cagagcgtaa agctcgtgat cgtctggcca tggaacgtca
      361 cctgggtatt gccaacgaaa taaaagatta tggctactat tcagtaatct acagaaccaa
      421 actacgcaaa tttattaaaa aattaaaagg caaagcgtaa accacacagt caaaacggaa
      481 ccaacatgga tttattagct gagagtatta ctgaagtcgc tgtctctggg gaaattgcta
      541 acaccgatcg tgtgttaaat atcgcttacg gtattgaccg taatttttta tttggtgcgg
      601 cagtatctat gcaatcagtt gttatgcata acccggacct tgcggttaag tttcatctct
      661 ttaccgacta cattgatgaa gattatctac aacgtgttaa cgcttttacc agcaaaaatg
      721 ctaacgttga agtaaaaatt tataaagtct ccagtgcctt tattgatatc ttccccagcc
      781 tgaaacagtg gtcttatgca acattcttcc gtttagttgc gttccagtat ctgagtgaag
      841 ttattgaaaa tctgttatat atcgatgctg atgtcatctg taaaggctca ttagctggat
      901 tgcttgatat taattttgat ggcgataagt tcgcagctgt tattaaagat gtgcctttta
      961 tgcaggaaaa accagcgaag cgtctggcta tagagggact tccaggtaat tatttcaacg
     1021 ccggtgtagt atatctgcag cttgaagcat gggcgaaaaa tgattttatg aataaagcca
     1081 ttgctatgct ggcaagtgac ccgcagcaca cgaaatataa atgccttgat caggatattt
     1141 taaatattct gttctttggt cattgtattt ttattagcgg cgattatgat tgcttttatg
     1201 gcattgacta tgagttaaaa aataaaagcg atgaagatta taaaaagacc attaccgatg
     1261 atactaagct gattcattat gttggcgtaa cgaagccctg gaacgactgg acgaattatc
     1321 cctgccagaa gtattttaat gaggcttatc aggcttcttg ctggaatgat gtggcgttta
     1381 ttccagccac gaatgaaaag cagtatcaag tgaaatacca acatgcaaag aaaaatggtg
     1441 atacgtttaa cgcttttatt ta
//
LOCUS       DAZGAP010000414         1461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGAP010000414 DAZGAP010000000
VERSION     DAZGAP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.417"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            176..1357
                     /locus_tag="ACHLAM_005353"
     CDS             176..1357
                     /locus_tag="ACHLAM_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7856703.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     T"
BASE COUNT          410 a          375 c          325 g          351 t
ORIGIN      
        1 tttcacttcc agattcatct cccgaactcc acgaaactca agtataatca catcgttaca
       61 agcaatctga acatcctgaa ctccaccgaa ctctactgaa ctctttctca atcaagcttt
      121 cattgggggc accggtgggg gctcattatg ttcagtcagt ttcaggagcc cccatatggc
      181 acttacagcc agacaggtcg aaacagccag acctaaagaa aaagactata aactctctga
      241 cgaacgtggt ttatatctgc tggtaaaaac cacgggagcc cgctactggc ggcttaaata
      301 ccggatagca ggaaaagaga aaaaactggc cctcggcgtc tatcccgacg tctcccttgc
      361 tgaggccaga atcaaacgcg acgatgcccg aaaaatcatc tccgaaggtg gtgacccggg
      421 cgaaaagaag cgaaaggaaa aactcactca gaaaatctct gccaccaata cgttccatgc
      481 cctcgctacg gaatggcacc agcataaatc tttgtcatgg tctgaaagtt acgccagaag
      541 cgtactggaa gcgctggata aagatatttt cccgtatctg ggcaaacgaa gcgttacgga
      601 tatcctcccg ctggaaatgc tggaaattct gcgccgcata gaaaaacgtg gctcgctgga
      661 aaaactgcgt aaggtacgtc aatactgtaa tcagattttt cgttatgcca tcgccaccgg
      721 acgagccact gtcaatccgg catctgaact gaccagtacg ctggcggcgc caaaagctgc
      781 acacttcccc cacctgagag cagatgagct ccctgttttt ctcaggaagc tcgctgaata
      841 tcatggcagt cctgttaccc gcatggcgac aaatctgctg cttctgacag gcctcagaac
      901 gattgagcta cggtccgctg aatggtcaga aattgatttt gataatgccc tgtggacaat
      961 ccctgaaagc cgcatgaaaa tgcgacgtaa acatgtcgta ccactgtcac gacaggtcac
     1021 tgacattctg caacaactca aaactttctc cggacaatac cggctggttt tcccgggacg
     1081 ttgtgatatc aacaagccaa tgagcgaagc cagcatcaat atggtgctca aacgtatcgg
     1141 ttacgatggc agggcaaccg gtcatggttt tcgtcacacc atgagtacca ttctgcacga
     1201 acaggggttt aattctgcct ggattgaaat gcagttagct catgtggata aaaattccat
     1261 caggggtacc tataatcatg ccctgtatat cgatggtcgc cgtgaaatga tgcagtggta
     1321 tgctgactac attgattcgc tttctgagca aacctgatga atcagtgaca taaaaataat
     1381 gaataaccat ctggccccga aagccggatg gttttattta tgttttcttt tttatttgct
     1441 cacatcttct gaactgcccc t
//
LOCUS       DAZGAP010000415         1455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGAP010000415 DAZGAP010000000
VERSION     DAZGAP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.418"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..276
                     /locus_tag="ACHLAM_005354"
     CDS             <1..276
                     /locus_tag="ACHLAM_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4526/YPO1902 family pathogenicity island
                     replication protein"
                     /protein_id="HFO7856704.1"
                     /translation="ARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGL
                     DVMMLAAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            291..704
                     /locus_tag="ACHLAM_005355"
     CDS             291..704
                     /locus_tag="ACHLAM_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO7856705.1"
                     /translation="MAEINFQVLREAEQLTGVATAGRPVARHFDDGLTLEVNRTCTTG
                     ERNANSALYGAVWRAAKAMGYRRCITYTRADESGASLRADGFVRVKELPSGKSWAESS
                     VALKSTRDPVGSGGVPRVLREIRRMSTTVIHIKGE"
     gene            708..1175
                     /locus_tag="ACHLAM_005356"
     CDS             708..1175
                     /locus_tag="ACHLAM_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856706.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGFLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            1193..>1455
                     /locus_tag="ACHLAM_005357"
     CDS             1193..>1455
                     /locus_tag="ACHLAM_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856707.1"
                     /translation="MTLPVDSLIAYTLEKMQERIEQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQL"
BASE COUNT          398 a          306 c          433 g          318 t
ORIGIN      
        1 gcgcgccgtc gcattgccgg tattgatgtt cgcccgggac gtggcatcac actgagtgat
       61 gaggaaagtg cagagctgtg gcgtttatgg cagaagagcg gcatcacgga tgtggagagt
      121 gcggacggac tggatgtcat gatgctggca gctgagcaaa tgaatatccc gctgacggcg
      181 gtatggcatg ccgtgagggg atggcatcag ggaactgcgg ggtttaatga tgtatcggcc
      241 agtcgccgga aactgaaccg ggaaaaagca gaatgacaag gacaaatgac atggccgaaa
      301 ttaactttca ggtactgcgg gaggctgaac aattgacagg tgtggcgaca gccggcagac
      361 ctgttgcacg ccactttgac gatgggctga cactggaggt caatcgtacc tgtactactg
      421 gtgaacgtaa cgctaacagc gcactttatg gcgctgtctg gagggcggca aaagctatgg
      481 gatatcgccg ttgcattaca tacaccaggg ctgatgaatc cggagcctct cttcgggcag
      541 atggttttgt tcgtgtgaaa gaacttcctt caggaaaaag ctgggcggaa tcaagtgtcg
      601 ccctgaagag tacacgcgat ccggttggaa gcggtggtgt tcctcgtgtg ctccgggaaa
      661 tcaggagaat gagcactact gtcattcaca tcaaagggga atgagatatg gctattgcag
      721 agagaacggg tatctcctcg cgcggcgata cttacgaaat ctggttggga gcaaactgtt
      781 atcactaccg ctctgatggt tatgcagtgc ttctgaaaga aggcaggaat cgtgcttatc
      841 catgtcgggt gacgagtgaa acgattaaat ctgcgctggc agcaattgtt gaattgcaac
      901 agaaattaac tgcggcagag aagaaaaagg agtgtgagaa ccacgggttt cttttgccag
      961 ctgagatgtt aattcgctgt ctggaagatc aaacaggggg acggtggatc cgtgcacatg
     1021 aaccagtaat gctgatgaag cgattaatca gtaacctgaa aaaagccaga ccagaatgta
     1081 ccgttcctga tgaagcgctg gcatatctcc ggcgtaactg tctggtgaat atgggcgata
     1141 tacccgtgac cagccgacgg atgtctgacg tataacaaaa aggaaaagtg taatgacatt
     1201 acccgtagac agcctgattg cgtacacgct ggaaaaaatg caagagcgga tagagcaaaa
     1261 aggccgacaa caggacggaa aaatacaaag cggactgctg tttaccggaa atgtgcatga
     1321 tgccattccg cgtcgactgc ttctggatac tcgactttct ccgctggata agatggcgtg
     1381 gatgatgatt cggctgtatg cacagaataa tgaaggtgca gttttcccca catatgacga
     1441 actgcaactg cagct
//
LOCUS       DAZGAP010000416         1451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGAP010000416 DAZGAP010000000
VERSION     DAZGAP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.419"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..1159
                     /gene="ompC"
                     /locus_tag="ACHLAM_005358"
     CDS             5..1159
                     /gene="ompC"
                     /locus_tag="ACHLAM_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7856708.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
     gene            1369..>1451
                     /locus_tag="ACHLAM_005359"
     CDS             1369..>1451
                     /locus_tag="ACHLAM_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7856709.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDL"
BASE COUNT          373 a          272 c          383 g          423 t
ORIGIN      
        1 aatcatgaaa aaaatgacag tggcactttc tgctgtagca gtggcagtga tgtttgctgc
       61 gggggcgcag gcagcagaag tttataataa agatggtaat aaactggatc tttacgggcg
      121 tgcaaccgct ctgcattact tctcggatga taaaggtaat gacggagacc agacttatgc
      181 tcgtctcggc tttaaaggcg aaacgcagat taatgatcaa ctgaccggat ttggtcagtg
      241 ggaataccag ttctctggta acaaaacgga atctgaaggt tccgcgggaa ataaaacccg
      301 tctggcattt gcaggtctga gatttgcaga tgttggtacc attgattacg gacgtaacta
      361 tggtattgct tacgatgtcg gatcatatac tgacgtactg cctgagtttg gtggtgatgg
      421 ctggacgcag accgataact ttatgacggc ccgaacttcc ggtgtcctga cttaccgtaa
      481 tacagacttc ttcggactgg ttgatggtct gaattttgcg gcgcagtatc agggcaaaaa
      541 tgagcgtgat gaccttcaga aggctaatgg cgacgggtat ggtttctcgg ccagctatga
      601 gtttgatggt tttggttttg tcgctgcgta taccaagtca gatcgtaccg ataagcaggt
      661 tcaggggttg aacggtactg cagatgttct tgatcctgaa tcaggtaaaa aggttggaga
      721 aaaaacagtt gactcaggta gtgtggcaaa aggtaaacat gctgaattct ggggaactgg
      781 tcttaaatat gatgccaata acctgtatct ggcagcagtg tattctgaaa cccagaatat
      841 gacgaccttt ggtgatcaag gtgttgcgga taaagctcag aatatcgaag ccgttgttca
      901 gtatcagttt gattttggtc tgcgcccgtc ccttgcctac cttcagtccc gcggacagga
      961 tgttatggtt ggtggtgtga accatggcga tcaggatctg gttaaatata ttgatgtcgg
     1021 tgcgacttat tactttaaca agaatatgtc cacctatgtt gattataaaa ttaacctgat
     1081 tgatgaaagt gaatttaccc ggaaagccgg tgttgcgaca gataatatcg ttgctgtggg
     1141 tatgacttat cagttctgat tattgctgat aagttaataa atacgggccg tctgccctta
     1201 caggcggcct gtatcaatga aaacacagtt ttcattggtc actgcgatca gcaattgcca
     1261 tctggcatgt gctgatttaa ctttctgtta ttacctttat tggttttatt ttaaattgga
     1321 cttttattgt tcggggcgcg tctgcgcccc ttttttatgg gcggatatat gcaggatatt
     1381 cacgaagaaa gcctgaacga gtcggttaag tcagagcagt caccgcgggt ggtgctctgg
     1441 gaaatcgacc t
//
LOCUS       DAZGAP010000417         1450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGAP010000417 DAZGAP010000000
VERSION     DAZGAP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.420"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..624
                     /locus_tag="ACHLAM_005360"
     CDS             241..624
                     /locus_tag="ACHLAM_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856710.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            717..>1450
                     /locus_tag="ACHLAM_005361"
     CDS             717..>1450
                     /locus_tag="ACHLAM_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HFO7856711.1"
                     /translation="MKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVG"
BASE COUNT          315 a          341 c          442 g          352 t
ORIGIN      
        1 ttgacagctt actgctgcag tttttgtccg gtcaggggcg acagttatcc ccggatgact
       61 gggcagcact ggaagaggcc gaatatcagc tttactggct gcgggctctt gccggtgggc
      121 ggtaacagaa cgccgtagtt tctttatccg gaataacgtc atcacgtatt ccgtcattat
      181 atcacccggc agggacatat ccctgtgggg agagtcccgg ctttattcag gagaattgtt
      241 atgggactcg atatttactt tctggctcgt gataaccatc acagtggtgc cgttgtggga
      301 acacaggaac atcaggaggt gggggctttc cgtaaggtga aaccgcttct gacatggttt
      361 cgtgaacata ccggtgcttc agatgagcag catgctttcc ctgtaacccg cagttcactg
      421 gctgcattat tgcgcgacct cagtatgctg acaccgggga actgccacag gctgtttcct
      481 tatgtcacag gctcgtgttt acggggggaa gattatgccg gtgattactg gtatgacgtg
      541 gatgacgtaa aagcgtgggt cagcaatacg ctgaagacgt ttgattttga gcgtcgttca
      601 ctggtgatgc tggcgttgtg gtaattctgg ccagtctgat tttcgttatt aatttaactg
      661 tacggtgcac tctcaccgta cagggagcgt gcatcttttt cagggggcat gctcttatga
      721 aaacgaaaaa acatacttca cgtaccgcga ctaaaactcc ggaacgtgac ctgtatcagc
      781 ttgtgaccga cagggttgtt acggccattg aaaatggtgt gccaccctgg aaacgtccct
      841 ggcgctctgc gcgtaatggc gtggggcttc cggagatgat gccggcaaat gccctgaccg
      901 ggaagcctta tacaggggtg aacgtcctgt tgctgtggct ggccgcggat gaagccgggt
      961 atgtcagcaa tgactggctg acgtaccgtc aggcgcaaca actgggtggc cacgtccgta
     1021 aaggggaggt ggccacgctg gcggtgattt acaaagactg gaaaaaacag gctgtggatg
     1081 aacacggtaa ccggctgacg gacagtgagg gggagcctct catggaaatc gtaccggtac
     1141 tgaaatcgaa tcctctgttt aatgtggggc agtgtgacgg tttacctgaa catctgatgg
     1201 tgaaggcgga aggcagcgcg gatgcactgc cggcagatac cttcagtgag cgggtgctgc
     1261 aggtactgaa tggcagcggc gtcagcgtct cgtcacttcc acaggacagg gcattctacc
     1321 ggcctgatct tgaccgtatc gtgatgccac tgtcgtcgca gtttgaccag gtggctgatt
     1381 actggaccac cctgctgcat gagctggtac acagtaccgg tcatgaaaag cgcctgcggc
     1441 gggtggggat
//
LOCUS       DAZGAP010000418         1448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGAP010000418 DAZGAP010000000
VERSION     DAZGAP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.421"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            508..753
                     /gene="yahM"
                     /locus_tag="ACHLAM_005362"
     CDS             508..753
                     /gene="yahM"
                     /locus_tag="ACHLAM_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO7856712.1"
                     /translation="MAVQLFKTLLNQIPLLSFLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(770..1441)
                     /gene="yahN"
                     /locus_tag="ACHLAM_005363"
     CDS             complement(770..1441)
                     /gene="yahN"
                     /locus_tag="ACHLAM_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO7856713.1"
                     /translation="MMKLLHLFMVEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
BASE COUNT          432 a          327 c          323 g          366 t
ORIGIN      
        1 ttgttaaatt aactccacta ctttacacgt tagttatgag cgatttctga tagtgtctgg
       61 tttaatcaga gctttattat ctgcgacgtt tatttttatt taatagagcc acctgcaagt
      121 tgttattcca ccagagagtg aatcgcaaag caggtaatca tttcatctgt atatctgtat
      181 gccaactggc attccatctc ttttttcgcc gatattttgt tgtgatcggc gacacttcgc
      241 ttaaaaaagc atcagcagtg gtttcgcagc tatgcggtgt ataaaaaatg atctcatgca
      301 gatgttttgt gaatgtgtgg gttgacattc atatgaaaaa aatcataatt ccatcatgtt
      361 tgtgtggggt cttttctgta tcttacgcat cgcactcaag cctgacagaa aatatgctgt
      421 aaggctcata tcaaaaaccg ccattagctc atcaggaaga gcagacgaca accaggattg
      481 ttgtatggta cggggttcga ggcctcgatg gcagtccagc ttttcaaaac tcttctgaat
      541 cagatcccct tattatcatt cctgcaaagt ggaacattgc cattgtttgg ctattcagga
      601 cgggtcagac caatgaaaaa agcccacaca ggggagagtg ggctgaaatg ggaagctaaa
      661 gactcaagta aacttatcgg aaataaggac cacgcattac gggggctatc atcgccgatg
      721 gtcgggattc ggcaaatcag gttgattacg tagagagtaa attatctgct caccgctgcg
      781 tcaccccttc gtaaatcagg cgtagcgcga atacaccaat aattgcacca ataacccgac
      841 tggcaacgcg ttgcatacgc ccataagcac gacgcactgc gggcaaagaa aacgcctgac
      901 taagaaaaac gcgccagata actgatgcga gcacaatccc cgcccaggcc attaaacgtg
      961 cccatgttgg tgtttcggca tttaatgtta ctgagaaaat actaataaaa aataaaacgg
     1021 tttgcgggtt agagagatcg gtaattaatc cacggcggaa aaagatatac cagggggcac
     1081 taatcggttg ttgtagtgtg ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg
     1141 caaaccataa gagataagcg ccgccgacga ttctgataag cgaaaaaatc tcctcacact
     1201 gcgtaattag tgttgcaaga ccaaacaaac ccaacccgga ataaaatgca tcgcccaacg
     1261 ccacgcccag tccggtcagc acccctgcgc gtcgaccgga agccaggctg gtttgtacta
     1321 ccacaaagag attggctccc ggattaaaaa aagtaatcac gaacagtcct acggtcagat
     1381 aaacggcgtg caaaggatcc atagtgattt caaccataaa taagtgaagt aacttcatca
     1441 tatttcta
//
LOCUS       DAZGAP010000419         1444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGAP010000419 DAZGAP010000000
VERSION     DAZGAP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.422"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..>1444
                     /locus_tag="ACHLAM_005364"
     CDS             197..>1444
                     /locus_tag="ACHLAM_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7856714.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TREVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKIN"
BASE COUNT          397 a          357 c          335 g          355 t
ORIGIN      
        1 attcatatca acggacctcc acggaggtcc gtttttcgtt tcagaacacc atgatttaaa
       61 tgttcttcct ccaaatcaat tctaccgaac tcaaccaaat tctccccaca tcaacctcat
      121 tgtgtgggta taattgcggg tataccccag ttcgacagaa tttgtaccct ctttaacgca
      181 ttcagaaggc tgaactatgg cactgactga cgcaaaaatc cgggctgcaa agcccactga
      241 caaggcttat aaactcactg acggggctgg catgttcctg ctggtacatc ctaatggttc
      301 ccgttactgg cgtctccgtt atcgtattct gggtaaagag aagactctgg cacttggtgt
      361 gtatccagaa gtatcgctct ccgaagctcg tacaaaacgg gatgaggccc gaaaactgat
      421 ttcggagggg gttgaccctt gcgaacagaa aagagctaaa aaagtagtcc ctgatttaca
      481 actctctttc gaacatattg cacgacgctg gcatgccagt aataaacaat gggcacaatc
      541 acacagcgat aaagtactca aaagcctcga gacacacgtt ttccccttta tcggcaaccg
      601 ggatatcaca acactcaata ccccggacct gcttatccct gttcgtgctg cagaagcaaa
      661 acaaatttat gaaatcgcca gtcgtctgca gcaaagaata tctgctgtaa tgcgttacgc
      721 cgtacagtct ggcatcatca gatataaccc ggctctggat atggctggtg cattgaccac
      781 ggtaaaacgc cagcatcgcc ccgctctgaa tctttcacgc ctgcctgaac ttctgtcgcg
      841 tattaacagt tataaaggtc agcctgtcac ccggcttgcc gttatgctga atttactggt
      901 ttttattcgt tccagtgaac tcagatacgc ccgctggtct gaaattgata ttgacaatgc
      961 catgtggact attccagccg aacgcaaacc tctgccaggg gtaaaattct cacaccgggg
     1021 ctccaagatg cgaacaccac atcttgtgcc actcagcaaa caggctgtag ccatactgac
     1081 agaacttcag acatgggctg gtgaaaatgg tctgatcttt acgggagcac atgacccgcg
     1141 taaaccaatc agtgaaaata ctgtaaataa agccctgagg gtgatgggat atgacacaac
     1201 ccgggaagtc tgtggccatg gattccgggc gatggcatgc agtgcattga ttgaatcagg
     1261 tttgtggtcc cgcgatgcag tggaacgtca gatgagtcat caggagcgta atggtgtacg
     1321 tgctgcgtat atccataaag cagaacatct ggaagaacgt cgactgatgc tacagtggtg
     1381 ggcagatttt ctggatgcaa acagggagga gtgtatcagt ccgtttgaat atgcaaagat
     1441 taac
//
LOCUS       DAZGAP010000420         1440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGAP010000420 DAZGAP010000000
VERSION     DAZGAP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.423"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..709)
                     /locus_tag="ACHLAM_005365"
     CDS             complement(<1..709)
                     /locus_tag="ACHLAM_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856715.1"
                     /translation="MPSLVAGFYISNYPGESDFRNELTENNIKIVLLTSLLSTLIFFG
                     YFFLMYNAGKEIEQYVLLQLSIIWICSFILSLCLNGSITYRQISTEGKWKQIKKFLSM
                     HIVQSTLFTHAACIYIIPLELFLHTLEFPEGINEFWQVLSVIVLSILLIIFSLIPCIV
                     FLRLRTRTSLLNQVIVTGGIMAGILAFIFTFVHMVPALILTIFLKISGIMDLTPHVYG
                     IPTTSYPAEYFRNSAWQS"
     gene            complement(1188..>1440)
                     /locus_tag="ACHLAM_005366"
     CDS             complement(1188..>1440)
                     /locus_tag="ACHLAM_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO7856716.1"
                     /translation="GFIVSLYATWLFFRRKKGTRKAMIAYYATGLIIALYLTLLPAIL
                     FDVQLDSDNIRLLVSGIFGVVIWIPYFLFSKRINMVFCR"
BASE COUNT          502 a          267 c          263 g          408 t
ORIGIN      
        1 cactctgcca ggctgagttt ctgaaatatt cagcaggata tgaggtggtc ggtattccat
       61 aaacatgagg agtcaagtcc atgattcctg atattttaag gaaaatagtt agtattagag
      121 ctggaaccat atgcacaaac gtaaaaatga aggctaatat tccagccatg attccgccag
      181 tcacaattac ctgatttagc aagctggtgc gggttcttaa cctgaggaaa acaatacagg
      241 gaataagtga aaaaataatc agaagaattg ataatactat aacactcaac acctgccaga
      301 actcgtttat tccttccggg aattccaacg tatgaagaaa taactcaaga ggaataatat
      361 atatgcaggc tgcgtgggtg aatagcgttg actgtacaat atgcatggaa agaaactttt
      421 tgatctgctt ccacttaccc tctgtgctta tctgtctata agtaatagag ccattgagac
      481 acagagaaag tataaaactg caaatccaga taatgcttaa ctgaagtagc acatattgtt
      541 caatttcttt tcctgcgttg tacatgagga aaaaataacc aaaaaatatc agtgttgata
      601 atagcgaggt cagaagaacg attttgatgt tgttttccgt cagttcattc cggaaatcac
      661 tttcccctgg ataattggaa atatagaatc ctgccactag tgagggcata aataacgcca
      721 tgcaaaatag gagtacgctg atacaagcaa agaccatcag ataactgaac aatgatgaaa
      781 aagaaatact gtcaaaaaaa agtcctgttt gtccaatacg cgcaagatgt gaccatataa
      841 acaatgctac aatagaagtt gacgcgatcg gtgtaaggat tgttaaaaac caagcacata
      901 gttttataaa atgtttgttt actctgtaat tttctatatt ttagtgtcca ttcaaagttt
      961 gacatatgat ctagctaatg actccatttt tgggcattgc ttaccaacat acctcttatg
     1021 tgtattactg tgaaggagaa cgatgaatag cgactattca cgtggatctc cacaacaacc
     1081 atcaatattc cagccattat cattcccggt atccttaccg gcattacctg tagaatcggt
     1141 tattgttgtt gccctataac acacttttgt ggctataacg tttatagcta tcgacaaaaa
     1201 accatattaa ttcttttgga aaaaagaaaa taaggtatcc atataacaac accaaatatt
     1261 ccactcacta ataatctgat attatctgaa tccagctgta cgtcaaaaag aatagccggt
     1321 aataaagtaa gatagagagc aataatcagt cctgttgcat aataagcgat catggctttt
     1381 cgagttcctt ttttgcgtct gaaaaacaac cacgttgcat aaagggaaac aatgaatcca
//
LOCUS       DAZGAP010000421         1439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGAP010000421 DAZGAP010000000
VERSION     DAZGAP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.424"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            146..1375
                     /locus_tag="ACHLAM_005367"
     CDS             146..1375
                     /locus_tag="ACHLAM_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7856717.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          353 a          317 c          309 g          460 t
ORIGIN      
        1 aatcagagac ataactggca cgtaaggttt gcaaccacta acccaccaat agagaggtag
       61 aaagggcgtt aatctcccat acttaacctg gtttatggta aattgccctc cattttgttt
      121 aatttgtaga tgatacgttc agataatgtc taatatttgg tcaaaagaag aaactctgtg
      181 gagtttcgcg ctctacggca cagccgttgg tgcaggcacg ctcttccttc ctattcagtt
      241 aggttcggcg ggggctgtgg tcctgtttat tactgctctg gtcgcctggc ctttaacata
      301 ttggccacat aaagccttat gccagttcat cctctcatcg aaaacatcag caggtgaagg
      361 gataacgggc gcggtaacac actactatgg caagaagatt ggtaatctga ttaccacgct
      421 gtacttcatc gccttttttg tcgtcgtgtt gatatatgca gtggcaatta ccaactcact
      481 tacagaacag ctggcaaagc atatggttat tgatcttcgc atccgtatgt tggtgagtct
      541 gggggttgta ttaatcctga atctcatttt tctgatggga cgccatgcca ctattcgggt
      601 aatgggattt ttggtattcc cattgattgc ctatttctta tttctttcta tttacctggt
      661 cggtagttgg caacctgatc tattaacaac ccaggtagag ttcaatcaga atacccttca
      721 ccagatatgg atatcgattc ccgtgatggt tttcgctttt agccatacgc ccattatttc
      781 tacgtttgcc atagacagac gtgaaaaata tggcgaacac gctatggata aatgcaaaaa
      841 aattatgaaa gtcgcttatc tcatcatctg cataagtgta ttgttctttg tctttagctg
      901 cctgctttct attccacctt cgtatattga agcggctaaa gaagaagggg ttaccatttt
      961 atcggcgctt tctatgctgc cgaacgcccc agcatggttg tcaatttccg ggattattgt
     1021 cgcagtagtt gcgatgtcga aatcattcct gggtacgtac tttggcgtta ttgaaggtgc
     1081 cacagaggtc gtcaaaacaa cattacagca ggttggtgta aagaaaagtc gtgcatttaa
     1141 ccgcgcacta tcaattatgt tggtatcgct gattaccttc attgtttgtt gcattaaccc
     1201 gaacgcgatt tcgatgattt acgcgatcag cggcccgctc attgccatga tacttttcat
     1261 catgcctacg ctgtcaacgt atctcatccc ggcgcttaaa ccctggcgtt ccatcggaaa
     1321 tctgattacc ctgatcgtgg gtatcctgtg cgtatcggta atgttcttta gctaatatcc
     1381 gttgtggatg gttcaaaatt gcttcaattt tgaaccatcc cagttttcat aatgttcat
//
LOCUS       DAZGAP010000422         1424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGAP010000422 DAZGAP010000000
VERSION     DAZGAP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.425"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..862)
                     /locus_tag="ACHLAM_005368"
     CDS             complement(<1..862)
                     /locus_tag="ACHLAM_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO7856718.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSLDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGM"
     gene            1059..>1424
                     /locus_tag="ACHLAM_005369"
     CDS             1059..>1424
                     /locus_tag="ACHLAM_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856719.1"
                     /translation="MNTEKQLPSGIESEVKYTLQFWLLSNGKVMTEKDGLCNPFPLNC
                     IESAHPFTPFQQNQWVELDTVGPNGPVKATFLVHSVLHKLKRQGGSLNDGNWPEYGRC
                     IAHWHTDVFVEKKALPDWLR"
BASE COUNT          356 a          409 c          359 g          300 t
ORIGIN      
        1 ccattcctcc gtcaccaagc gccataccga ccgcagatga caccctcagc cagtcctcat
       61 catccatgtt gccactgcgc aaatgattca gttccacacg ccccagcatg gaaagcatgc
      121 gcagaatcag ttgctccttc ggcatctcaa tgctgaatac cagaacgctc tcttccgttc
      181 gccgcagtgc ggcgctgaga cacatggata acgccagaga ggttttcccc attgacggac
      241 gggcagccag cagaaccagg tcaccaggct gcaggccaca ggtcatttca tccagacagt
      301 caaatcccgt gggggtaccg gtaacaccac cggagccgag cacactttcc agttgctgga
      361 gaaccgtatc aagcgcggat gaaagagagg cttccgtgcg ctcccggagc gtcctgttct
      421 gcgcaagcgc aaacagacgc cgctctgcag catccagcac atcccgtgac gtggcatttg
      481 gtgccgccac atcagcaatc agggtgtttc cggtggcctg taactgacgc agaacgcttt
      541 tctccaccac aatgcctgca taggccatga tgttggccgc agaaggtgtg ttcttgctga
      601 gctcggccag ataagcaaag ccgcccagta cgtccagcct gccctgatgt tccaggtgct
      661 ctgacagtgt aatcaggtcc agtggctggc cattcaccgc aagttcagac atggcacgga
      721 aaatggcccg gtgctgagcc acataaaaat cgtcaagact cagcatcagc accacttcat
      781 cccagcgttc attctccagc atcagaccgc ccagaacggc ctgctctgct tcgtatgcgc
      841 agagaacaga catttcagcc atggcgtacc ctccctctct gactgccgga aacctggcta
      901 cgtgaaaaaa ggaaaaaggg tgccggacgt acagctgtcg ggaataaaat cccggtaact
      961 gtctgtacgt tttcagacca gtaagtctgg caaccccggt ctgaaagtac agacagcatt
     1021 cgttcattca taacaagttg ttttaaagga aaaagtttat gaatacagaa aaacaactgc
     1081 cgtccggcat tgaatctgag gtgaaataca ccctgcagtt ctggctgctg agtaacggaa
     1141 aggtcatgac ggaaaaagac gggctctgta acccctttcc cctgaactgt attgaatcgg
     1201 ctcatccttt tactccgttt cagcagaatc agtgggttga actggacact gtcgggccga
     1261 atggaccggt gaaagcgaca ttccttgtgc acagcgttct gcataaactg aaacgtcagg
     1321 gtggctccct gaatgatggc aactggcctg aatacggccg gtgcattgct cactggcaca
     1381 ctgacgtgtt tgttgagaaa aaagcgctgc ctgactggct gaga
//
LOCUS       DAZGAP010000423         1414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGAP010000423 DAZGAP010000000
VERSION     DAZGAP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.426"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1414
                     /locus_tag="ACHLAM_005370"
     rRNA            <1..>1414
                     /locus_tag="ACHLAM_005370"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          393 a          300 c          442 g          279 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcctctttat ggggtgactg
      181 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      241 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      301 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      361 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      421 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      481 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
      541 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
      601 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
      661 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
      721 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
      781 gcggcagcga cactaagtgt tgttgggtag gggagcgttc tgtaagcctg tgaaggtgta
      841 ctgtgaggta tgctggaggt atcagaagtg cgaatgctga cataagtaac gataaagcgg
      901 gtgaaaagcc cgctcgccgg aagaccaagg gttcctgtcc aacgttaatc ggggcagggt
      961 gagtcgaccc ctaaggcgag gccgaaaggc gtagtcgatg ggaaacaggt taatattcct
     1021 gtacttggtg ttactgcgaa ggggggacgg agaaggctat gttggccggg cgacggttgt
     1081 cccggtttaa gcgtgtaggc tggttttcca ggcaaatccg gaaaatcaag gctgaggcgt
     1141 gatgacgagg cactacggtg ctgaagcaac aaatgccctg cttccaggaa aagcctctaa
     1201 gcatcaggta acatcaaatc gtaccccaaa ccgacacagg tggtcaggta gagaatacca
     1261 aggcgcttga gagaactcgg gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga
     1321 aggcacgctg atatgtaggt gaagcgactt gctcgtggag ctgaaatcag tcgaagatac
     1381 cagctggctg caactgttta ttaaaaacac agca
//
LOCUS       DAZGAP010000424         1408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGAP010000424 DAZGAP010000000
VERSION     DAZGAP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.427"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(220..405)
                     /locus_tag="ACHLAM_005371"
                     /pseudo
     CDS             complement(220..405)
                     /locus_tag="ACHLAM_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            629..1369
                     /locus_tag="ACHLAM_005372"
     CDS             629..1369
                     /locus_tag="ACHLAM_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO7856720.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
BASE COUNT          372 a          299 c          339 g          398 t
ORIGIN      
        1 accttaaaaa tagcttttca cgcacaccaa aaaggatgac gatgggaagt ggattaatct
       61 caaaataata cttctaaatt aaaaaggtta aaagcaaatc acaaccttcc tgagcttccg
      121 cttttctctt tcagagggct gaagtcgtta tctgtaacca gaggagatca gtagcccgtt
      181 atgccagcta aagcactccg ggcagattgc ccggagtgct taatatttta atggattgtt
      241 aatcttcgca tactcaaagg ggctggtatt ttctttcgag tttgtatcca gaaaatcccc
      301 ccaccactgt agcatcaacc gacgctattc caggtgttct gttttatgga tataagccgc
      361 ccttaatgaa ttacgttcca tgtggctcat ctgacgttca gccgctttat ctcgtgctgt
      421 tttggtctta atacttccat gccgctgcga ttacctctta aaccagccct taccattttt
      481 atggggacga taagaaaact ttatcgggca cgatcgattt tatcgatcga ttcgatcgaa
      541 aacattcctt ttaccctgtc ttttgcatgg ttttagaatg agcaacccca tttatcgttc
      601 ggggaaagtg atagcggaga tgattgaaat gaataaggtt attgcggttt cagcgcttgc
      661 catggcaggc atgttttcga cccaggctct ggctgatgag agcaaaacag gcttttatgt
      721 gaccggtaaa gcaggtgctt ctgttatgtc acttgcagac cagcgtttcc tgtcgggtga
      781 tggagaggaa acatcaaaat ataaaggcgg tgatggccat gatacggtat tcagtggcgg
      841 tatcgcggcc ggttatgatt tttacccgca gttcagtatt ccggttcgta cggaactgga
      901 gttttacgct cgtggaaaag ctgattcgaa gtataacgta gataaagaca gctggtcagg
      961 cggttactgg cgtgatgacc tgaagaatga ggtgtcagtc aacacactga tgctgaatgc
     1021 gtactatgac ttccggaatg acagtgcatt cacaccatgg gtatctgcag ggattggcta
     1081 cgccagaatt caccagaaaa caaccggtat cagtacctgg gattatgggt acggaagcag
     1141 tggtcgcgaa tcgttgtcac gttcaggctc tgctgacaac ttcgcatgga gccttggcgc
     1201 gggtgttcgc tatgacgtaa ccccggatat cgctctggac ctcagctatc gctatcttga
     1261 tgcaggtgac agcagtgtga gttacaagga cgagtggggc gataaatata aatcagaagt
     1321 tgatgttaaa agtcatgaca tcatgcttgg tgtgacttat aatttctgac aaaactgctc
     1381 ctgaaagata ataattaccc atcttctg
//
LOCUS       DAZGAP010000425         1405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGAP010000425 DAZGAP010000000
VERSION     DAZGAP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.428"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1405
                     /locus_tag="ACHLAM_005373"
     CDS             <1..>1405
                     /locus_tag="ACHLAM_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7856721.1"
                     /translation="ITEDAHYLYRYDEYGRLAEKTDRIPAGVIRTDDERTHHYHYDSQ
                     HRLVFYTRIQHGEPLVESRYLYDPLGRRTGKRVWRRGRDLTGWMSLSRKPEETWYGWD
                     GDRLTTVQADTTRIQTVYQPGSFTPLIRIETDVTELEKAQRRSLAEKLQQEGSEDGHG
                     VVFPAELVRLLDRLEEEIRTDRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARKVHLY
                     HCDHRGLPLALISEDGNTAWSGEYDEWGNLLNEENPHHLHQPYRLPGQQHDEESGLYY
                     NRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAV
                     SGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADV
                     IGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGI
                     KADNPIKAKQGSSDASNY"
BASE COUNT          368 a          329 c          440 g          268 t
ORIGIN      
        1 atcaccgaag acgcgcacta cctgtaccgt tacgatgaat acggcaggct ggcggagaag
       61 acggaccgca tcccggcggg tgtgatacgg acggacgacg agcggaccca ccactaccac
      121 tacgacagcc agcaccgcct ggtgttctat acgcggatac agcatggcga gccactggtc
      181 gagagccgct acctttacga cccgctgggc cgccggacgg gtaaacgggt gtggcgtcgc
      241 gggcgtgacc tgacaggatg gatgtcgctg tcgcgtaaac cggaggagac gtggtacggc
      301 tgggacggtg acaggctgac gacggtgcag gccgacacca cgcgtatcca gacggtatac
      361 cagccgggaa gcttcacgcc gctcatccgc atcgagacag acgtgaccga actggagaaa
      421 gcgcagcgcc gcagcctggc ggagaagctc cagcaggaag ggagcgagga cggacacggc
      481 gtggtgttcc cggctgaact ggtgcggctg ctggacaggc tggaggaaga aatccggaca
      541 gaccgcgtga gcagtgaaag ccgggcgtgg cttgcgcagt gcgggctgac ggtggagcaa
      601 ctggaaaaac aggtggagcc ggaatacaca ccggcgcgaa aagttcatct ttaccactgc
      661 gaccaccggg gactgccgct ggcgcttatc agcgaagacg gcaatacggc gtggagcggg
      721 gagtatgatg agtggggaaa cctgctgaat gaggaaaacc cgcatcacct gcaccagccg
      781 taccgtctgc cagggcagca gcatgatgag gagtcggggc tgtactataa ccgtcaccgg
      841 tactacgatc cgttgcaggg gcggtatatc accccggacc cgattgggtt gagaggtgga
      901 tggaatatgt atcagtatcc gttgaatccc atacaagtga tagacccaat ggggttagat
      961 gcgattgaga atatgacatc aggtggacta atttatgccg tatctggtgt acctggattg
     1021 attgctgcaa acagcattac taacagtgct taccagttcg gttatgatat ggatgctatt
     1081 gttggcggag ctcataatgg ggccgccgat gcaatgagac attgttactt gatgtgtcga
     1141 atgactaaga catttggatc aacaatagct gacgtgatag gtaaaaatca tgaggcggca
     1201 ggggatagac aaggtcagcc agctaaagaa agaatcatgg atcttaaaaa taacactgtc
     1261 ggtattgctt gtggcgattt ttctgccaaa tgtagcgatg catgtattga aaaatataac
     1321 actgggcaac tcttcgggtt agatggtata aaagcagata atccaataaa agcaaagcaa
     1381 gggagttcag atgcttcaaa ttatt
//
LOCUS       DAZGAP010000426         1397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGAP010000426 DAZGAP010000000
VERSION     DAZGAP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.429"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..110
                     /locus_tag="ACHLAM_005374"
     CDS             <1..110
                     /locus_tag="ACHLAM_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO7856722.1"
                     /translation="NLLGKRDKRRAKILASKNYILWRMVRLVSRKKNKR"
     gene            156..>1397
                     /locus_tag="ACHLAM_005375"
     CDS             156..>1397
                     /locus_tag="ACHLAM_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO7856723.1"
                     /translation="MSFCWNEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLTRMNDLNRDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVALTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCL"
BASE COUNT          425 a          239 c          243 g          490 t
ORIGIN      
        1 gtaatcttct tggcaagaga gataagaggc gagcaaaaat attagcatca aaaaactata
       61 ttctctggag gatggtaaga ctggtaagta gaaaaaagaa taaacgttag gtcttgcatt
      121 aaataaccca tcctcgtagc ataggttgaa attatatgtc gttttgttgg aatgaaatta
      181 actctggtgt caagtcttta attctcatat tatgtatgtt gtctttaatg actttgtctt
      241 tgtgggatga tgttgcaaca aagtttcttc atgcagctgg aattatatct gcattatatt
      301 ttctggcgac accaaaaaaa acaataacta ataatcctac tttgttaatt ttcatctcat
      361 tatgtctttt gggtatcgtt aatatcatct ggtattcaca ttataaagtt tcaggctcgg
      421 tttataccaa tgcatatcgt ggcccaatgg aaactggaaa aattgccttg tgtagtgctt
      481 ttattttctt agttcttttt gctaaagatg agatgagaac caaaataaaa tttgggaaac
      541 taattctgtt cgcatccctg gcaacgcagt tacttttttt tgcgcatgcc atgtggcaac
      601 atttctattt aaacgtcgac cgtgttgcat tatcagcgtc ccacgctaca acagcaggct
      661 acatcatcct tttcccttct ttactggcat caattctcat tttaaaatcc gactttagac
      721 ataaaacaac attatataca attaacttca tgcttagctt atgtgctgtt atagtaactg
      781 aaacgcgtgc agccatatta gtgtttccat tctttgcgtt actattaatc gtaatggata
      841 gttatattaa taagcgaatt aattataagt tatattgttt tattgcgatt gcattattag
      901 caggtgtatt ttcttttaaa gatacattac ttacgagaat gaatgactta aatagggatt
      961 tagttaatta ttcgcatgat aacaccagaa cttcagtcgg tgcccgtctg gcaatgtatg
     1021 aagttggttt aaaaacatat tctccaatag gacaatcact ggaaaaacgt gcggaaaaaa
     1081 tacatgaact agaagagaaa gagcctagat taagtggcgc tttacccttt gtagattctc
     1141 atttgcataa cgatctcata gatacgttat caacgcgtgg tattcctgga gtagcattaa
     1201 caattttagc cttttcagca atattcatat atgccttaag aactgctaaa gaaccttata
     1261 ttttaatctt gcttttttca ctactggtag taggcctaag tgatgtaata ctcttttcta
     1321 aaccggttcc gactgctgtg tttgtcacca taatattgct ttgtgcttat tttaaagcac
     1381 aatcggacca atgttta
//
LOCUS       DAZGAP010000427         1365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGAP010000427 DAZGAP010000000
VERSION     DAZGAP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.430"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(154..963)
                     /gene="pduF"
                     /locus_tag="ACHLAM_005376"
     CDS             complement(154..963)
                     /gene="pduF"
                     /locus_tag="ACHLAM_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFO7856724.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
BASE COUNT          475 a          326 c          247 g          317 t
ORIGIN      
        1 atttaatctt cacatcatcc ttgatgattt actgacgaga agcacaaagg acaataacaa
       61 aagcctccgt gaagcgggag actttttaat aagttcggaa agcagatcac aaacagtaaa
      121 aaaataaacg tttataactg ccagattatt tcgttattcg ctgatttctt taggcgttac
      181 actattgaca ggtaaattct ttgcaatgag atagcgataa atcgccgcac caacacatgc
      241 cccaatgatc ggtgcaataa tgggtacaat gaaataagga atattgcgtc caccagtcat
      301 cgcaatttcc ccccaacccg caaaccaggt gaaaatttta ggtccaaaat cacgcgctgg
      361 attcatggca aaaccggtta atggtccggt agaagcacca ataacagcaa ccagaatacc
      421 aatgagtaaa ggcgcaagcg gtcctttggg tacgccatta ccgtcatcgg tcaacgccat
      481 aatcaacccc attaacatgg acgttatgac cacttcgacc aacgctgctt gccatacatt
      541 aatcgccgct gctggataag tactgaagat actggcgagt tgtaaacttt ctgcgctacc
      601 acgcactata ttgtgagcag attcaaaatc ggtaaacaaa ctgctgtaga gagaatatgc
      661 cagaaccgca ccaccaaagg cacccgctaa ttgcgccaca caaaatggca gcactttacg
      721 ggctgggaaa gaggcaaaca gccacagagc aatggtaact gccgggttga gatgagcgcc
      781 ggaaatacct gccgtaaggt aaacagcaag cgaaatccct aatccccaaa tgatacaaat
      841 ttcccacagt cccagacttg ccccggtaac cttcagggcg cacaaacaac caatgccaaa
      901 aaagaggaac agtccggtgc ccaaaaactc ggcaacgcat tgcgccttga gtgaatcatt
      961 catagtgaca ccttctttac ggagtttttc tttatcagcc aactatgcgc tgattgttgt
     1021 gtataccgag cgcaactata aaaaattgct ctgcagaaaa tattgccaac atgcatgaca
     1081 gactcgtgta aaaatcgtgc taacataaca aattattgtc attgccgaat catgcaacac
     1141 agcgttaatc agtgttgaaa gtaataattt ccaccaaact tttacaataa aaaattgcca
     1201 tcatgacata ataaaattga aacaaatatc acagttcctt ccttcgaacc aaactcaacg
     1261 aaatattact cgcgattaaa ctcactagcc aacgcaatta attcacattg aaatatctac
     1321 aaaacaacca ataaattaac aacatatagt tcataaaagc aattt
//
LOCUS       DAZGAP010000428         1364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGAP010000428 DAZGAP010000000
VERSION     DAZGAP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.431"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..879
                     /locus_tag="ACHLAM_005377"
     CDS             <1..879
                     /locus_tag="ACHLAM_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HFO7856725.1"
                     /translation="GSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVN
                     GGVHKTPDNVADAVNMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRGQNSIAM
                     GNGAEVTRQAAAGSVAVGSHARAGRANTGTYALNGQAVAGRTGSDTAVVSFGQPGQER
                     QLQSVAPGVLSANSTDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDI
                     RGVGASAAAMSGIPQAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQ
                     NTRGNFSVGAGVGWQW"
     gene            904..>1364
                     /locus_tag="ACHLAM_005378"
     CDS             904..>1364
                     /locus_tag="ACHLAM_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO7856726.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTL"
BASE COUNT          337 a          283 c          437 g          307 t
ORIGIN      
        1 gggagccagc tggcatctgc agccagaatt gctggcggtg ataatgttgt ctggaaagac
       61 ggaaaagcag cgctggctcc tgacaccttc agtgagctga caactgcagg tggcggtaag
      121 acaacggtga atggtggtgt acataaaaca ccggataatg tggctgatgc agtgaatatg
      181 ctgaaccgtg aaggcacgaa gtattttaaa gctgcctctt ccggtcaggc agcaaaagcg
      241 gaagggcgtg acagtattgc tgtcggacag ggggccgttt cccggggaca aaacagcatt
      301 gccatgggta atggtgctga agtcacgcgt caggctgctg ccggctctgt tgcagttggc
      361 agccatgccc gtgctggccg cgcgaatacc gggacttatg ccctgaacgg tcaggctgtt
      421 gcgggacgta ccggaagtga tactgcggtg gtttcttttg gtcagccggg acaggagcgt
      481 cagctgcagt cagtggcacc gggtgtgttg tctgcgaatt caacggatgc cgtcaatggc
      541 agtcagttgc atgcaactaa ccgtcaggtt gccggaaata cacaggcgat aaacactctc
      601 gggaataaat tcagtcagct gagctaccgg gtggaagagc ttaaccggga tatccgtggt
      661 gttggcgcat cggctgcggc catgagcggg atacctcagg cctatcttcc gggtaaaagt
      721 ctgatggggc tgggtgtcgg tggatatggt ggtgaaagcg ccattgcaat aggtgtgtcc
      781 cgaatctccg ataacggtaa gatgataatg aaactgaatg caggtcagaa cacccggggt
      841 aatttctccg tcggtgccgg tgtgggctgg cagtggtaac agggaaaagc aaggagaaat
      901 gtaatgttat ttagtcgaat gatgatgacg ggtgtgctgt ccctgatgct tgcaggatgt
      961 ggaaatctga gtcatgtcac ggatgaagga acgacggaag aagtggtatg gccttctccg
     1021 gagaaggtga tgtttaatca tgacggaaca cagaaaggtt cgtggccact gccagaaaag
     1081 ttacgtcaga taaaggcggg tatgaataag gaccagattt atcagctggc ggggagacca
     1141 cactttgctg aagggatgtc tggtgtaagg gaatgggatt acctgttcaa ctttaccgga
     1201 ccggaggggg attatacctg ccagtacaag atactgtttg accggaacat ggatgcccgc
     1261 tctttctact ggaagccgga aaactgtctg ccggaggcag agacagcaga atacagggag
     1321 tcagtccccg gtgatatatt atttgatttt gattcatgga ctct
//
LOCUS       DAZGAP010000429         1364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGAP010000429 DAZGAP010000000
VERSION     DAZGAP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.432"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1045)
                     /locus_tag="ACHLAM_005379"
     CDS             complement(<1..1045)
                     /locus_tag="ACHLAM_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7856727.1"
                     /translation="MLKDGTGKPVPGCTIELKARRTTEMVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSTTDSDDETKAATPKAVKAA
                     MDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWKTREFVAWLKEKGA
                     FDQPYWMMKASLLASMNKVITDVGPGKLNLGGCVIEVMGTYEAAIVRVTIGEYGATGF
                     INGTVCTCTVYGDT"
     gene            complement(1209..1349)
                     /locus_tag="ACHLAM_005380"
     CDS             complement(1209..1349)
                     /locus_tag="ACHLAM_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7856728.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
BASE COUNT          238 a          465 c          293 g          368 t
ORIGIN      
        1 gtgtgtcgcc gtaaaccgta caggtacaga ccgtgccatt aataaacccc gttgcaccgt
       61 attcgccaat ggtgacccgg actatggcag cctcatacgt tcccatcacc tcaatgacac
      121 agccacccag attgagtttt cccggtccga catccgtgat gactttattc attgacgcaa
      181 gcagtgatgc cttcatcatc cagtaaggct ggtcaaatgc ccctttttct ttcagccagg
      241 caacaaactc gcgtgttttc cattcaccgg cccccgtatg aatgtcccgc ccgtatactc
      301 tggctgctcc cacggtgttc agaaacttca ccttatccgg gatatcgtca ccgtttttcg
      361 ctttctccag gcatccgtcc gctttgtcca ttgccgcttt caccgctttc ggggtggctg
      421 ccttcgtttc atcatcactg tccgttgtgc tgcttaactg cacaattccc ttctgtgccg
      481 tcgtcgcatc cggccctccc ggctcgcctt tttcgccctt ctctccccgt tcacctttca
      541 caccctgaag cccctgtggt cctgtctcac cacgctcacc ctttggcccc cgctcgccgg
      601 tatcgccttt tggcccggga ataccctgcg gcccggtgtc ccccttatcc cccttcggtc
      661 cccgcgcatt ctctgcccgt ttttttgcct cctccgcact ggctgcggac gcttccgcac
      721 gtttcaggat ttccgctgcc accgcttcca gctctgcaag ggctttcggg taatactgtg
      781 cgtcttccag gtccatcaga aatttattca gcgttcccgg tgcagaatct gccttcacca
      841 gaatgtcacc cacatatgac ggcgcgtacc cttccgtgtt cagcgtcacc cggtataacc
      901 cgggctcaac atccatactg taactgccgg tttcccccgg ctgaccatac gccaccgtgg
      961 tgacaatcac catctccgtt gtgcggcgcg ctttcagctc tatcgtgcat cccggtactg
     1021 gttttcccgt accatccttc agcacacccg atattctgac tgtcatggat ttcccccata
     1081 aaaaaaccgc agtaccggtt tcccggcctg cggtaatatt tgtggtttgt tggtgttaaa
     1141 acggtgccat ccggctgacc accctcagca accggtcggc gggggatatt ctcccccgcc
     1201 acggtttctt actgcttaca ctgtaagaac gccgcaatct ccgcgcccgc tatccggaac
     1261 cggaactcgc acagtgaact gtgggtgatc cagataatga gcactaccgt gatacaaatc
     1321 acggtggttt ttaacggttt ttgcgacata aacgcttgct cctt
//
LOCUS       DAZGAP010000430         1361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGAP010000430 DAZGAP010000000
VERSION     DAZGAP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.433"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(48..626)
                     /gene="flhC"
                     /locus_tag="ACHLAM_005381"
     CDS             complement(48..626)
                     /gene="flhC"
                     /locus_tag="ACHLAM_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO7856729.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(629..979)
                     /gene="flhD"
                     /locus_tag="ACHLAM_005382"
     CDS             complement(629..979)
                     /gene="flhD"
                     /locus_tag="ACHLAM_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO7856730.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT          356 a          317 c          316 g          372 t
ORIGIN      
        1 gcggtacgtc gttaccgctg ctggaatgtt tcgccacacc gtatcagtta aacagcctgt
       61 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
      121 ctttttactg cccgggatgg cggctgacat aagctgcagg caaagctgcc aacaggctgg
      181 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
      241 ctttcaacaa accgcaccaa tgtccaggca cgggttaatg ccagcagcgg tccctcttct
      301 gcttgcgggc actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta
      361 cacaaaccgg ttttcagtaa aaactgccat gcgttacaga acatcgaagc atgaacgttt
      421 tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt
      481 ccgcgcagtt ctttatagag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc
      541 agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat atcccgcgct
      601 tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag
      661 gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga
      721 gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtgactg tcaaaacgga
      781 agtgacaaac cagttgattg gtttctgcca gcttaaccat ttgcggaaga gtcagtgccg
      841 ctaacgttgt cgccatttct tcatttatgc cgagacgaaa catagcggac gctttgtcct
      901 gaacaatcag acgctgtgca agtagtaaat atgacaagtt gatgtcataa atgtgtttca
      961 gcaactcgga ggtatgcatt attcccaccc agaataacca actttatttt tatgcggtct
     1021 caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa ataattagca ttagaatagt
     1081 tgcgataagc tgcaataggc agagcccctt tgtgggattt aataagtcct tacaaataga
     1141 aggggttctt tacacttatc taagattttt cctaaatcga cgcaacttta ctcgtcacta
     1201 cacgcacata caacggaggg gggctgcgat tttcaataat gtgtgatgca gatcacacaa
     1261 aacgctcaat tacttaacat aaatgtataa gtcatacttt tgttttgggt gtatttcaat
     1321 ctgttaaaaa gtttttcgcg acgctagcaa gcaaaaatga a
//
LOCUS       DAZGAP010000431         1337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGAP010000431 DAZGAP010000000
VERSION     DAZGAP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.434"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            347..>1337
                     /gene="shiA"
                     /locus_tag="ACHLAM_005383"
     CDS             347..>1337
                     /gene="shiA"
                     /locus_tag="ACHLAM_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO7856731.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSA"
BASE COUNT          291 a          322 c          334 g          390 t
ORIGIN      
        1 gcaatacgtc tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata
       61 atgcaatgca gtctatcaga ctcatcaggg tgccattttg tgcatatgcg gacttttatg
      121 tttcatatct cgaacctgtg aggcctctgc ttaatcctta aacaacacca gcaactcctg
      181 cgctttcatc ttccagcgaa tttttcatgt tgctgctcat cagccataaa atcatttgca
      241 gatgcgctct gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt
      301 accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct
      361 catctccact cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt
      421 aggcagtttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc
      481 cgcactggtg tttaatcgcg agtttttccc gcaagtaagc ccggctatgg gaacgctcgc
      541 cgcatttgcc acctttggcg tcggattcct gttccgtccg ctcggcggtg tcattttcgg
      601 tcactttggc gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg
      661 catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc
      721 tattttgctg gtgacactgc gtgccattca gggatttgcc gtcggcggcg aatggggagg
      781 cgcggcgttg ctttccgttg aaagtgcgcc gaaaaataaa aaagcctttt acagtagcgg
      841 tgtacaagtt ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag
      901 tatgatgaca actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag
      961 catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt
     1021 tgaacaacag caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct
     1081 gttacgacat cccggtgctt tcctgaagat tattgcacta cgactgtgcg agttgctgac
     1141 gatgtacatt gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg
     1201 cgaacttttc cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg
     1261 ttttgcctgg cttgccgatc gttttgggcg gcgcagggtt tatatcacag gtgcgttaat
     1321 cggaacgttg agcgcat
//
LOCUS       DAZGAP010000432         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGAP010000432 DAZGAP010000000
VERSION     DAZGAP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.435"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..219
                     /locus_tag="ACHLAM_005384"
     CDS             <1..219
                     /locus_tag="ACHLAM_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO7856732.1"
                     /translation="RILQGLPDGPFTRKQAEAVAAQYRNVFIEDDNGEHFRLVVRNNG
                     AMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            283..>1325
                     /locus_tag="ACHLAM_005385"
     CDS             283..>1325
                     /locus_tag="ACHLAM_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7856733.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEDVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDAL"
BASE COUNT          302 a          356 c          411 g          256 t
ORIGIN      
        1 agaatacttc agggactgcc tgacggtcct tttacccgca aacaggccga agccgttgcc
       61 gcacagtacc ggaacgtctt tatcgaggat gacaacggag aacattttcg cctggttgtc
      121 cgtaataacg gtgcaatggt ctggcgtacc tggaattttg aggacggtgc cgggtactgg
      181 atgaaccatg tcatcaggga tttcggcatt cttaagtaag agaggtgccg gacgggtagg
      241 cgtccggcag acatacaacc accccggtca aaggagaaat atatgtcagt tacagagtct
      301 aaggcaaaaa cagagcgcaa atccagccgt aaacctgcaa aaacgcagga aactgtcctg
      361 tctgccctgc tggcgcagac ggaggatgtg agcgttccgc tggcttcact gattaaatca
      421 ccgctgaatg tgcgcacggt gccgtattct gcggagtccg tcagcgaact ggctgagtcc
      481 attaagggcg tcggcctgct gcagaatctg gttgttcatg ccctgccagg tgaccgccac
      541 ggtgtcgccg caggtggtcg ccgactggca gcactcaaca tgctggcaga gcgtggcatc
      601 attccggctg actggcctgt ccgcgtgaaa attattccgc aggaactggc gactgccgca
      661 tcaatgactg aaaacggtca tcgtcgggat atgcaccctg ccgaacagat tgccggattc
      721 cgcgcaatgg cgcaggaagg caaaacacct gcacaaatcg gtgatttgct gggctattca
      781 ccccgccacg ttcagcgaat gctgaaactg gcagaccttg cacctgtcat ccttgatgcg
      841 ctggcagaag accgcatcac cacagagcac tgtcaggcgc tggcgctgga gaacgacacc
      901 gcgcgtcagg tgcaggtgtt tgaagccgcc tgccagtcag gatggggcgg taaaccggaa
      961 gtgcagacca ttcgtcgtct ggtgaccgaa agtgaagtgg cggtggcggg gaacagtaaa
     1021 ttccgcttcg tgggggctga tgccttctcg ccagatgaac tgcgcactga cctgttcagc
     1081 gacgacgagg gtggctatgt ggactgcgtg gcgctcgatg ccgccctgct ggaaaaactc
     1141 caggctgtcg ctgaacacct tcgggaagcc gaaggctggg aatggtgcgc cggacgtatg
     1201 gagcctgtcg gttcctgccg tgaggatgcc ggaacatacc gcagcctgcc ggagccggaa
     1261 gcggtgctga cggaagctga agaagaacgc ctgaacgaac tgatgacgcg ctacgacgcg
     1321 ctgga
//
LOCUS       DAZGAP010000433         1315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGAP010000433 DAZGAP010000000
VERSION     DAZGAP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.436"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1315
                     /locus_tag="ACHLAM_005386"
     CDS             <1..>1315
                     /locus_tag="ACHLAM_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO7856734.1"
                     /translation="DTPSFSSVQLNGKGNNGNVTLSSEGNKLTLSGGNGAGQNGVVIS
                     GVASGLEGKELSTLTNTDKEMKNAASVADLKTAIDGVSTSMQGAGFALKDSDDKEVKQ
                     SLGQAIKVSGDSNITTTAITNGDKGLKISLNNDLSVGSNKAPGSIKVNGTDGKEAVSL
                     NGQDGTIKLTSAPAANGKSASATIAVNAGKGGLTDAVGTTKTRLTYTPDGKSPAEELA
                     TMNDGLSFEGDDGKIIHKKLNEKLTIKGGAGDNEDVTDSNIRVDANEKGDGLLVRMTR
                     NLRDLASAVFGTENGPTTRIDNAGITITTPAAGGGQGNTVSLTGSGLNNGGNQITSVA
                     SGLNGTALADAGGDVLNNAANIGDLKNALRDSSAALVNTGFGLQADDGSKVNTSLGKH
                     ITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVGGTRMDKNG"
BASE COUNT          376 a          274 c          385 g          280 t
ORIGIN      
        1 ttgatactcc gtcattcagc agtgttcagc tgaatggaaa aggaaataac ggaaatgtaa
       61 cgctctcatc cgaaggtaat aagctgacat tgtcaggcgg taatggtgcc ggacaaaatg
      121 gtgttgttat cagtggtgtg gcaagtggac tggaaggtaa ggaactttcg acgttaacga
      181 atactgataa agagatgaaa aatgccgcca gtgttgcaga cctgaaaacg gcaattgatg
      241 gtgtgtccac cagtatgcag ggtgcaggtt tcgcactgaa agactctgat gataaggaag
      301 tgaaacagtc tcttgggcag gcgattaaag tatccgggga cagcaatatc accaccacag
      361 caatcaccaa tggggataag gggttaaaaa tatccctgaa taacgacctg agcgtgggga
      421 gcaataaggc tcccggcagc atcaaagtta acgggactga tggtaaggag gctgtgagtc
      481 tgaatggtca ggatggcacg attaaactga catccgcgcc tgctgcgaac ggaaagtctg
      541 cgtcagcaac cattgctgtt aatgcaggca aaggtggatt aacagatgct gtcggtacca
      601 cgaagacacg tctgacttat accccggatg gaaaatcacc ggcagaagag ctggcgacca
      661 tgaatgacgg tctttctttt gagggtgatg acggaaagat tattcataaa aaactgaatg
      721 aaaaactgac catcaaaggt ggtgctggtg ataatgagga tgtaacagac agtaacattc
      781 gtgttgatgc aaatgaaaag ggagatggtc ttctcgtccg gatgaccaga aacctccgtg
      841 atttggccag cgcggttttt ggtacagaaa acggcccgac aacccggatt gacaatgcag
      901 gcatcacaat cacaacacct gctgcgggcg gtggtcaggg gaatacggtt tcccttaccg
      961 gctccggcct gaataacggt ggaaatcaga taaccagtgt ggccagtggt ctgaacggca
     1021 ctgcgcttgc tgacgccggt ggagatgttc tgaataatgc ggccaatatc ggtgacctga
     1081 aaaatgctct ccgtgacagc agtgcggcac tggtgaatac cggctttggt ctgcaggcgg
     1141 atgacggaag caaggtgaac acgtctctcg ggaagcatat cacggttggt ggtgacgggc
     1201 agaatatcag cacgactgtt cagaacggga gcctgacggt cagactcgct gataacattc
     1261 gcctggcaga tgacggcagt gtttctgtcg gcggaacccg gatggataaa aacgg
//
LOCUS       DAZGAP010000434         1305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGAP010000434 DAZGAP010000000
VERSION     DAZGAP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.437"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            488..696
                     /locus_tag="ACHLAM_005387"
                     /pseudo
     CDS             488..696
                     /locus_tag="ACHLAM_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            683..872
                     /gene="tnpB"
                     /locus_tag="ACHLAM_005388"
                     /pseudo
     CDS             683..872
                     /gene="tnpB"
                     /locus_tag="ACHLAM_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(875..1063)
                     /locus_tag="ACHLAM_005389"
     CDS             complement(875..1063)
                     /locus_tag="ACHLAM_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO7856735.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
BASE COUNT          342 a          336 c          270 g          357 t
ORIGIN      
        1 gaaccatctc cctggatatc cccatcacat tgcatgaaaa gcaagtttta ccaatcttga
       61 ttttcagtgt taaataagat taataactgt aattctgcaa aaaaaagaac accatatgta
      121 gtatgactac gtattccact ctgttcgttc acggactggc atcgaagtct gaggtgtact
      181 ggcaatagcg gatacagtca tttgttcttt ttttaagcat tcatccgatg atacttttcc
      241 tgaatgctac cggagtggta ttcccctgcc gtgagggagg gcgctgacgg ttataaaaat
      301 cctgatatat acccattccc gtatgacagc gatggcgtca catctctgtt ctaccatctt
      361 cacagaaata tttcgttcat tagcattccg gtattcgtat aaccaccttc ggtggagaaa
      421 gaataaacaa tccctggaat gcgatgagcg cttgaccaga aaagcatcat attgcttcat
      481 ggcgcatcag cgaccacagg atgccgcaaa agccgaaaaa cggaccagag cgcctgcaat
      541 cattcctcgc ctcagtatct gttgcccatg gagtggccga taacagatca ttgtcggatg
      601 agccttatcc ccttccttcc cggtggtatc cggatgtgct gccagccttc tcagttaacc
      661 gatgttttcc gggctttttg ccatactgag gactgataac gtattcctgg tcgtaaaacc
      721 cgtcaatatg cgccggggca tcgacaaaac tgacacagta cgtccggaaa gaacttaacg
      781 ctgcatggca cgacaatgct gcctttgtct ttaccaacaa ggctcgttcc cgcataaaag
      841 ttctacgccg ggataaacat ggcgtatggc tgcgtcaggc cttcactact tcacaccgga
      901 agtcttccac cctgacacga atataccgtc cgggctcttt cgcaggtgca aacagttttt
      961 cacattcggc gaatgtcatt tctgaatcct ggtaacgcaa ccactgaacc ggcatattac
     1021 cgggtttgat tattgtcgca gttattttat atcgtttccc catatcgcct ctcatcctga
     1081 caaaaaaatt accggctgag ccatcactcc ggtaaagcat gcgaaaaaaa gtgagcgtct
     1141 cagcaatggc tcatgcgatg attctgtacg ggcaccgatg gcacctcaga atacaacaaa
     1201 gatatcagag taacatgtct ctctcgttat ttttcagagt tcttgacacc ctccttctgc
     1261 cacattgtca acgccagtgg agacttctgc tgtcagtttt ttcat
//
LOCUS       DAZGAP010000435         1303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGAP010000435 DAZGAP010000000
VERSION     DAZGAP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.438"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            140..754
                     /gene="evgA"
                     /locus_tag="ACHLAM_005390"
     CDS             140..754
                     /gene="evgA"
                     /locus_tag="ACHLAM_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO7856736.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            759..>1303
                     /locus_tag="ACHLAM_005391"
     CDS             759..>1303
                     /locus_tag="ACHLAM_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7856737.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQS"
BASE COUNT          410 a          260 c          259 g          374 t
ORIGIN      
        1 actatttata tggtatactt gttgaattat cttaaaggaa gctcaaattt tcttattttg
       61 attgagaaaa tgagatgatg ccttatgtct gtattactac agggagaagg gaaatgcttc
      121 attgcaaagg gaataatcta tgaacgcaat aattattgat gaccatcctc ttgctatcgc
      181 agcaattcgt aatttattga tcaaaaacga tattgaaatc ttagcggagt tgactgaagg
      241 gggaagtgcc gttcagcggg tggaaacact taaacctgat atcgtcatca ttgatgtcga
      301 tatccccgga gttaacggta tccaggtgtt agaaacgctg aggaagcgcc aatatagcgg
      361 aattattatt atcgtctccg ctaaaaatga ccatttttac gggaaacatt gtgctgatgc
      421 tggcgctaat ggtttcgtga gtaaaaaaga aggcatgaac aatatcattg cagctattga
      481 agctgcaaaa aatggctact gctatttccc cttctctctc aaccggtttg ttggaagttt
      541 aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa gaaattagtg tcatgcggta
      601 tattcttgat ggcaaggata ataatgacat tgctgaaaaa atgttcatca gcaacaaaac
      661 tgtcagcact tataaaagtc gcttgatgga aaaattagaa tgcaaatcac tgatggatct
      721 ttacacattc gcacaacgta acaaaatcgg ctaaccacat gaagttttta ccctatattt
      781 ttcttctctg ttgtggtctt tggtcgacca taagtttcgc agacggagat tacatcgaat
      841 atcgtggcat cagtagtaac aaccgtgtca cacttgatcc actacgtctg agcaacaagg
      901 aattacgttg gctcgcgagc aaaaaaaatc ttgtgattgc agtacacaag tcccaaacag
      961 ctacgttgtt gcataccgat tcgcagcaac ggattcgtgg tattaatgct gattatttaa
     1021 atcttttaaa aagagcgtta aatatcaaat taacactccg ggaatacgca gatcatcaaa
     1081 aagcaatgga cgcgctggaa gacggtgaag ttgatatagt gttatcacat ttagttgctt
     1141 cgccgcctct taatgatgac attgctgcaa ccaatcctct gataattacc tttccggcgc
     1201 tggtcactac ccttcacgat tcaatgcgac cgcttacctc atcaaaacca gtaaatattg
     1261 ctcgagtagc aaattatccc ccagacgagg taattcatca atc
//
LOCUS       DAZGAP010000436         1299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGAP010000436 DAZGAP010000000
VERSION     DAZGAP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.439"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(46..279)
                     /locus_tag="ACHLAM_005392"
     CDS             complement(46..279)
                     /locus_tag="ACHLAM_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO7856738.1"
                     /translation="MPGCTARLLPEGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIR
                     NAEGQLRWRCWNFESDAGKQLNVWLSSEGLLRQ"
     gene            complement(350..>1299)
                     /locus_tag="ACHLAM_005393"
     CDS             complement(350..>1299)
                     /locus_tag="ACHLAM_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO7856739.1"
                     /translation="RILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPE
                     SSGSYGFVRLEGDLLITEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLG
                     GYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGWGWLGSLETGLPFSITDNLM
                     LEPQLQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRAPL
                     RDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAG
                     LEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          272 a          385 c          370 g          272 t
ORIGIN      
        1 gcgtctcccg gagaagatgc ggcctgatgg atgaaatgaa gacgtttatt gcctgagaag
       61 cccttcactg gagagccaca cattaagctg ttttccggcg tcagactcaa aattccagca
      121 ccgccagcgt agctgccctt cggcattacg gataaccagc cggaaatatg ttccctggtc
      181 atcttcagta aacacattgc ggtaagctgt tgtgacggca acagcctgtc cgcgtgagaa
      241 agggccttcg ggcaataaac gtgctgtaca acccggcata tctgttgctc cctgaaagta
      301 aaaagccccg gtcatgatga ccggggcctg aaggggagtg acctgattat cagaaagtca
      361 cattcagcgt ggcctgaccg ttatagcctt cagcgctgct gccgctgacg ctgtgggcat
      421 aaccggcctg aacgcccagg gtgatatttt cccggacacg ggcttccagt ccggcctgca
      481 ggtccagtga cgtgccattc tgggacggtg agaacgtcat cccgctgcct gcggtggagg
      541 tccccacacg catatcaccc cgggagctga aggtgcggat aacggaaggc tgtacccacc
      601 agttcaccgg cagttcacgc acactgtgtt ttgcactgtc acgcaggggg gcacgggatg
      661 aggtgccttt accaaagttc atatcgttgt ggctgcccag acggaagccg gcacgcacat
      721 gttgtgcact gccatgcccg aacttcacat aaccggcgtt atcctggccg tcatccaggg
      781 aaagtccctg ccaggtgtac tgcagttgtg gctccagcat caggttgtca gtgatactga
      841 agggcagacc ggtttccagt gagcccagcc agccccagcc ccgtgcgcgg aagtcgttat
      901 tgcccgatga cgctttcata ctgtggcggg ttccctgtgc cactatgtca gcccacaggc
      961 cggaggaggt gtgtgtcagg ttcatatatc cgcccaggct gccggcatca tcccggaccg
     1021 tgccggcgcg ggagccgtca tcatccttaa catcaacgga agaatggcct gcagcaccat
     1081 acaccccagt cgtcagtgac ataccggcga cctctgttat gagcaggtca ccctccagac
     1141 ggacgaagcc atagctgccg ctgctttccg gcgtggcccc acgggcaata ccgccgttgt
     1201 tatcgtgacc gagatgaccg ccctgaatgc tgagacggac gctgttattt tcaccgttta
     1261 caccggtctg atggctgcgg gagcctgcca gaatccggt
//
LOCUS       DAZGAP010000437         1296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGAP010000437 DAZGAP010000000
VERSION     DAZGAP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.440"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /locus_tag="ACHLAM_005394"
     CDS             complement(<1..353)
                     /locus_tag="ACHLAM_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7856740.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIA"
     gene            complement(444..1262)
                     /locus_tag="ACHLAM_005395"
     CDS             complement(444..1262)
                     /locus_tag="ACHLAM_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7856741.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT          286 a          345 c          365 g          300 t
ORIGIN      
        1 gcaatgccgg cggcttccgg gctcatttca gcgcggttac cgttcatggc attgaacagt
       61 acccagtttt cgccatcatc gtcatccggt tcgggggcca taaatgcgcc accgttgttc
      121 agtgtgtaca gattccagat accaccgcag tagtcttcgc aaagacggtc catccagccg
      181 aagatacggg gctccagggt cacccactgt ggaatgaggc caaaatgctg cggccagaag
      241 ctgatgcgct gttcatcagg gactatggtg gcaaccagct gaggctggtc actccctgat
      301 gcagttgtta cggaaacaga aggtgtggtg gagctatgcg aaaaggttgt catgaaatga
      361 ttccttataa aaagcaaatg aatgaagaag actcgggggc agggacagac gtgagtcagg
      421 acggcgcttt caggtaaacg gtatcagcgc atgctctcca gcagcgtttc cgccatcacc
      481 cacaatgcgc gattgagctt gatgtcggtg tcgatactgt gaatggcacg ggtatggata
      541 cgttttcctt ttgcactacg gccggaaatc ccgcctttca gcatattctc ctggatggtc
      601 tgatatgcgc tccacaggtc cttaccgtaa tcctcccggc gtcgtggtgt cagaatgtcg
      661 gcagtggtga cgggttgatg ttcatcacca taacggtaag tcagcgccgc ctgtgccagt
      721 gcctgacgtg ccggtggcgg cagaaccaga gactgcatgg catcacgctt ttcctcaatc
      781 cggtcaaaaa cgcccactac ctcgtaagcc ccttcaatga ctctgtccac cacatttccc
      841 cggtggggca cacggacttc ccccagagac tgaccgcaga cgcagccgtt ctggcagacg
      901 aatctgaagt aacccggcag catctggtag ctggaggtac cgtcatggga gttgagcaga
      961 ataatttcag gcacatgctg accggttatc tgtccggccc gccgcagacg cagcatgtgt
     1021 ttggtatatt cccggcggcc cgggtcacgt acgcgggtct ggcaggcgaa gaatggctga
     1081 aagccttccc gctgcaggct ttccagcacg gtgatggtgg gaatccaggt atagttctcg
     1141 cttctggatg tgtgtttctc ttccccgaat acgctgggga cgacctgcat cagttcttca
     1201 cgagttaagg ggcggtcacg gcgtatctgg ttcgcataac caaaacgact ggctaatcgc
     1261 atgatttatt ccttatgaat gagttagcgg tataaa
//
LOCUS       DAZGAP010000438         1281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGAP010000438 DAZGAP010000000
VERSION     DAZGAP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.441"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1281
                     /locus_tag="ACHLAM_005396"
     CDS             <1..>1281
                     /locus_tag="ACHLAM_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7856742.1"
                     /translation="IAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQ
                     HRLVFHTRIQHGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWD
                     GDRLTTVQTDSTRIQTVYQPGSFTPLIRIETDVTELEKAQRRSLAEKLQQEGSEDGHG
                     VVFPAELVRLLDRLEEEIRTDRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARKVHLY
                     HCDHRGLPLALISEDGNTVWSAEYDEWGNLLNEENPHHVYQSYRLPGQQHDEESGLYY
                     NRNRYYNPLLGRYITQDPIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLN
                     HQRAWDILSDTYDDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRD
                     YGLNMFGMYGRKVKLSHSEMIEDNKKDLAVNEHGL"
BASE COUNT          336 a          284 c          415 g          246 t
ORIGIN      
        1 atcgcggagg atgcgcacta tgtctaccgc cacgatgaat acggcaggct gacggagaag
       61 acggaccgca tcccggcggg tgtgatacgg acggacgacg agcggaccca ccactaccac
      121 tacgacagcc agcaccgtct ggtgttccac acgcggatac agcatggcga gccactggtc
      181 gagagccgct acctctacga cccgctgggc cgccggacgg gaaaacgggt atggcggcgg
      241 gagcgtgacc tgacggggtg gatgtcgctg tcgcgtaaac cggaggtgac gtggtacggg
      301 tgggacggcg acaggctgac gacggtgcag actgacagca cgcgtatcca gacggtgtac
      361 cagccgggaa gcttcacacc gctcatccgc atcgaaacag acgtgaccga actggagaaa
      421 gcgcagcggc ggagcctggc ggagaagctc cagcaggaag ggagcgagga cggacacggc
      481 gtggtgttcc cggctgaact ggtgcggctg ctggacaggc tggaggaaga aatccggaca
      541 gaccgcgtga gcagtgaaag ccgggcgtgg cttgcgcagt gcgggctgac ggtggagcaa
      601 ctggaaaaac aggtggagcc ggaatataca ccggcgcgaa aagttcatct ttaccactgc
      661 gaccaccggg gcctgccgct ggcgcttatc agcgaagacg gcaatacggt gtggagcgcg
      721 gagtatgatg aatggggaaa cctgcttaat gaggagaacc cgcatcatgt gtatcagtca
      781 taccgtctgc cggggcagca gcatgatgag gagtcggggc tgtactacaa ccggaaccgg
      841 tactacaatc cgttgctggg acgatatatt actcaggacc cgattgggct ggcgggggga
      901 tggagtctgt atgcgtatcc gctgaatccg gtgaatggta ttgatccatt aggattaagt
      961 cctgcagatg tagcgttaat gcgaaaaaaa gaacaattaa accatcaaag agcatgggat
     1021 atattatctg atacttatga tgatatgaag agattaaatt taggtgggac tgatcaattt
     1081 ttccattgta tggcattttg tcgagtgtct aaattaaatg acgctggtgt tagccgatca
     1141 gcgaaagggc tgggttatga aaaagagatt agagattacg ggttaaatat gttcggtatg
     1201 tacggcagaa aagtaaagtt atcccattct gagatgattg aagataataa aaaagacttg
     1261 gctgtaaatg aacatgggtt g
//
LOCUS       DAZGAP010000439         1252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGAP010000439 DAZGAP010000000
VERSION     DAZGAP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.442"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..300)
                     /locus_tag="ACHLAM_005397"
     CDS             complement(<1..300)
                     /locus_tag="ACHLAM_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856743.1"
                     /translation="MELTVDLLKSLNNIRQNATIYVHAYHYTDDEKCAMVPRISLPSL
                     KLTPLQSEIIIRLLLNGVDYIRHDGYVQGYREKTVATFTIQGEGRHRFKADLSQNG"
     gene            917..1144
                     /locus_tag="ACHLAM_005398"
     CDS             917..1144
                     /locus_tag="ACHLAM_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFO7856744.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
BASE COUNT          351 a          236 c          280 g          385 t
ORIGIN      
        1 cccattttgt gataagtctg ctttgaaacg atggcgtccc tccccctgta ttgtgaaagt
       61 tgccacagtt ttttctctat acccctgaac atatccgtcg tgccttatat aatcgactcc
      121 attgagaagc agtcgaataa ttatttctga ttgcaacgga gttaatttca gtgatggtaa
      181 agatatccgt ggcaccatag cgcatttttc atcatcagta tagtggtatg cgtgaacata
      241 tatggtggca ttttgcctga tgttgttaag gcttttcagc aggtctacag tcaactccat
      301 cgcggtatcc ttcaggttgt tatcattcgt tgatgatgcg atctgttgaa ggcgttggcg
      361 acaattaatt ctttttctct tctgaaaaat ttacggaata caaaaaagat ctgtttttaa
      421 ctgattgtta ctttctgaag atattctctg ctccatttgc tttccgggta agacgtatgg
      481 ctggttatgg tatggtcgga ctgcagccac agggggatga ctgtaatacc tctgatcacc
      541 acagtcagtc atctgtaaat gttaaatacc tcacagttca gtcagtggat tattgtcggt
      601 cttacgggat atttgttaaa aaaatactgc atggtgaatc cccctgagcg gcggggcata
      661 tcagtatcgt aggtgtttgg tcacatccct tatctgtgcg ggttcaggtg ctgatactga
      721 actcaccggg aggcacccgg caccatgcta cataacactc cattacgcta cactcgcccc
      781 tctccggagg ggttttctct gcaggatgtg aatatgagat gctgcattat tcactgcgaa
      841 tattgatggt gagaatcttt aataatatta atgtttatat gtattgttac agtgttgatg
      901 aaaggagata aaaacaatgt tcccggagta cagagaactg atttcagcat tgaaaggaaa
      961 tcataaacgc tttgattctt tatttgaaaa gcataataaa cttgatcacg aaataaaaag
     1021 aattgaagat gaatttggtt cagcgtattc gacgaaagtg gtcaggctga agcttgaaaa
     1081 acttgagatc aaaagagaaa tccagaaaat ccttgaagat gagtcagaaa agggcgaacg
     1141 gtaatcataa acggtatatt caaagacgtg ttttctctgt tttatgaaaa aggcaccggg
     1201 ggctccacgg tgccttttag cctgtgtata acgaaaagga gatcctgttc ag
//
LOCUS       DAZGAP010000440         1232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGAP010000440 DAZGAP010000000
VERSION     DAZGAP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.443"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..949
                     /locus_tag="ACHLAM_005399"
     CDS             <1..949
                     /locus_tag="ACHLAM_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO7856745.1"
                     /translation="RILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPE
                     SSGSYGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLG
                     GYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGWGWLGSLETGLPFSITDNLM
                     LEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPL
                     RDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAG
                     LEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          255 a          353 c          365 g          259 t
ORIGIN      
        1 ccggattctg gcaggctccc gcagccatca gaccggtgta aacggtgaaa ataacagcgt
       61 ccgtctcagc attcagggcg gccatctcgg tcacgataac aacggcggta ttgcccgtgg
      121 ggccacgccg gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg acctgctcag
      181 aacagaggtc gccggtatgt cactgacgac aggagtgtat ggtgctgcag gccattcttc
      241 cgttgatgtt aaggatgatg acggctcccg cgccggcacg gtccgggatg atgccggcag
      301 cctgggcgga tatatgaacc tgacacacac ctcctccggc ctgtgggctg acattgtggc
      361 acagggaacc cgccacagta tgaaagcgtc atcgggcaat aacgacttcc gcgcacgggg
      421 ctggggctgg ctgggctcac tggaaaccgg tctgcccttc agtatcactg acaacctgat
      481 gctggagcca cggctgcagt acacctggca ggggctttcg ctggatgacg gtaaggacaa
      541 cgccggttat gtgaagttcg ggcatggcag tgcacagcat gtgcgtgccg gtttccgtct
      601 gggcagccac aacgatatga cctttggtga aggcacctca tcccgtgccc ccctgcgtga
      661 cagtgcaaaa cacagtgtgc gtgaactgcc ggtgaactgg tgggtacagc cttccgttat
      721 ccgcaccttc agctcccggg gagatatgcg tgtggggact tccactgcag gcagcgggat
      781 gacgttctct ccctcacaga atggcacatc actggacctg caggccggac tggaagcccg
      841 tgtccgggaa aatatcaccc tgggcgttca ggccggttat gcccacagcg tcagcggcag
      901 cagcgctgaa ggctataacg gtcaggccac gctgaatgtg actttctgat aatcaggtca
      961 ctccccttca ggccccggtc atcatgaccg gggcttttta ctttcaggga gcaacagata
     1021 tgccgggttg tacagcacgt ttattgccga aggctctttt gctgccaaat ggcatctgaa
     1081 tcgatcacag tctgaccggt tattacccga tcccgatggg gttattgggt gatctccttc
     1141 cataaaaata cgcagctcgc cagtgaaggg cttctcagac aataaacgtc attcatttcc
     1201 cccatcaggc cgcgtcttct ccgggagacg cg
//
LOCUS       DAZGAP010000441         1225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGAP010000441 DAZGAP010000000
VERSION     DAZGAP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.444"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            883..>1225
                     /locus_tag="ACHLAM_005400"
     CDS             883..>1225
                     /locus_tag="ACHLAM_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856746.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAI"
BASE COUNT          342 a          236 c          316 g          331 t
ORIGIN      
        1 aatttcctga actagtctga atcgacaaga agtagcactt ttctccagag ggcgctctac
       61 ggcataagcg cttcctgtgc ggagctttga ttgataatcc agcattcttt acctggcgtg
      121 tattaccgtg aaaaacgccg ttatgggtga tctccttcta tgaaaatatg cacaggcttc
      181 tttctgggaa cagttctgct gtacattaag caagagtttt tcttaccggg ggccataaca
      241 gtactcccac agttgctcta tctccagtac cacagacaag ccataagaag ataaagaggc
      301 cactatgact gggggtatgc tgtttgttac ggctattgta gtctgagtgt atagggaaaa
      361 taaaaatggg cgactgacca gatatgggag tctctggtca gtcattcgct tgtagatgca
      421 ggacataagc gaacccaggc ccactgcttc tgcgcaaaag catagagagt ttacactggt
      481 agattctgtt gcgccagata tacaggagta agcgcacgca ccgtctgtga cgtcttgtga
      541 tggttgtctt ttttggtaac aatcgcgtcc agaatggtgt ggagccagaa tcgtgtttac
      601 gtgaagcgat catatggatc aataataatc catcgaacag ggtatacgaa caatggggga
      661 agacatgctg aaaagccgat tttcagaggc tgattgaaga agggattgtg agtttttcct
      721 tcggctttta acgtaacttc ctgttttccc gatattccac ggcaagatta atttttagcg
      781 gagctgaatg aataaaatct tcatctggtg tttttgttgc gcgtaaatgc aggtgtaact
      841 gtggatgtta tccactgaag agctgctgat gaggatgaag acatgctgac taaaaaaatg
      901 ttactcgttt gtgcttcgtg tgttgcgctg agtgcctgtt ctgcacagga aattgcagag
      961 ctgaacaggc agataagtga cggtgcattt gctgtgacaa aagtactgaa cgtaggtgta
     1021 aaccaggcag atgatgatct caaatatctg tctcaggcac cccgggcaaa aagagagaaa
     1081 cgtgatcatg tcatagaagt ccctgttgat gtggatacag ctgcgggaag actgaaacgg
     1141 tactataagt tttattcaaa tgaagaagtc gatgcgattc gtaataacgg acatgcgaac
     1201 gggaagtggg ccgcagcagc aataa
//
LOCUS       DAZGAP010000442         1216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGAP010000442 DAZGAP010000000
VERSION     DAZGAP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.445"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..1207
                     /locus_tag="ACHLAM_005401"
     CDS             join(45..306,306..1207)
                     /locus_tag="ACHLAM_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFO7856747.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          307 a          312 c          344 g          253 t
ORIGIN      
        1 gacacgcggc taagtgagta aactctcagt cagaggtgac tcacatgaca aaaacagtat
       61 caaccagtaa aaaaccccgt aaacagcatt cgcctgaatt tcgcagtgaa gccctgaagc
      121 ttgctgaacg catcggtgtt actgccgcag cccgtgaact cagcctgtat gaatcacagc
      181 tctacaactg gcgcagtaaa cagcaaaatc agcagacgtc ttctgaacgt gaactggaga
      241 tgtctaccga gattgcacgt ctcaaacgcc agctggcaga acgggatgaa gagctggcta
      301 tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa atgaagtatg tctttattga
      361 aaaacatcag gctgagttca gcatcaaagc aatgtgccgc gtgctccggg tggcccgcag
      421 cggctggtat acgtggtgtc agcggcggac aaggataagc acgcgtcagc agttccgcca
      481 acactgcgac agcgttgtcc tcgcggcttt tacccggtca aaacagcgtt acggtgcccc
      541 acgcctgacg gatgaactgc gtgctcaggg ttaccccttt aacgtaaaaa ccgtggcggc
      601 aagcctgcgc cgtcagggac tgagggcaaa ggcctcccgg aagttcagcc cggtcagcta
      661 ccgcgcacac ggcctgcctg tgtcagaaaa tctgttggag caggattttt acgccagtgg
      721 cccgaaccag aagtgggcag gagacatcac gtacttacgt acagatgaag gctggctgta
      781 tctggcagtg gtcattgacc tgtggtcacg tgccgttatt ggctggtcaa tgtcgccacg
      841 tatgacggcg caactggcct gcgatgccct gcagatggcg ctgtggcggc gtaagaggcc
      901 ccggaacgtt atcgttcaca cggaccgtgg aggccagtac tgttcagcag attatcaggc
      961 gcaactgaag cggcataatc tgcgtggaag tatgagcgca aaaggttgct gctacgataa
     1021 tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa tgtatccatg gagaacactt
     1081 tatcagccgg gaaataatgc gggcaacggt gtttaattat atcgaatgtg attacaatcg
     1141 gtggcggcgg cacagttggt gtggcggcct cagtccggaa caatttgaaa acaagaacct
     1201 cgcttaggcc tgtgtc
//
LOCUS       DAZGAP010000443         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGAP010000443 DAZGAP010000000
VERSION     DAZGAP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.446"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..608
                     /locus_tag="ACHLAM_005402"
     CDS             <1..608
                     /locus_tag="ACHLAM_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856748.1"
                     /translation="ASVSVKKVGLSEGDALKNTGVDKQEDDYSRHVGTEESKSKVSGT
                     EQNKNKGKTPPKVDRNVLVPSYVKFNFRGHKKCSRIFNELKSHLTFDNTPNAISILLR
                     IFIDLSVSTFVEENKLQFKEPHRTPGLHDKVKMCANFLRENKKMTGSQCTAVITFSSQ
                     ITKHDGSLQQYVHNPHLIPSKEAVNTEWDNFELLLSLIWSE"
     gene            678..>1212
                     /locus_tag="ACHLAM_005403"
     CDS             678..>1212
                     /locus_tag="ACHLAM_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO7856749.1"
                     /translation="MKFYTPLRYPGGKGKLSYFLKDVIEKNSLNDGAYAEPYAGGAGV
                     ALELLLEEYVRKIYINDADFAVYSFWSSVVSDTDELCRLISRAKIDMDEWSFHRYVIS
                     NPSEFTKLEVGFAAFFLNRTNRSGILKAGVIGGKSQNGKWKMDVRFNRADLISRIEKI
                     ANYNQRIVVTNLDALDFL"
BASE COUNT          397 a          172 c          269 g          374 t
ORIGIN      
        1 atgcatctgt gagtgtaaag aaagtaggtc tttcagaagg tgatgcctta aagaatacag
       61 gtgtggataa acaggaagat gattattcta gacatgtagg cacagaagag agtaaatcta
      121 aagtttcggg tacggaacag aataaaaaca agggaaaaac tcctcctaaa gtagatcgta
      181 atgtgttggt gccatcttat gtgaaattta attttagagg gcataagaaa tgctctagaa
      241 tatttaatga gcttaagtcg catctgacat ttgataacac ccctaatgct atctcaatat
      301 tgttgagaat atttatagat ctctctgtaa gcacttttgt tgaagaaaac aagttacaat
      361 ttaaggaacc acatagaact ccagggcttc atgataaggt taaaatgtgt gctaatttcc
      421 ttcgggaaaa taaaaagatg acggggagtc aatgtacggc tgtaataact ttctcgagcc
      481 aaataactaa acatgatggt tcattgcagc agtatgtaca taacccacac ttaattccct
      541 caaaagaggc tgtcaacact gagtgggata actttgagtt gttattaagc cttatttgga
      601 gtgagtgaga tttgtctaca tctggacatg gatgtccaga aactgataaa ctacaaaatc
      661 aggaatgaga gggatttatg aaattttata ctccattaag ataccccggc gggaaaggta
      721 agttatctta ctttttaaaa gatgttattg aaaaaaactc gttgaatgat ggagcatatg
      781 cagaacctta cgctggtggt gcaggggtgg ctttagaatt attattggaa gagtatgtcc
      841 gtaaaatcta tataaatgat gcagattttg ctgtttattc tttttggtca tctgttgtta
      901 gtgatactga tgagctttgc cgacttatta gccgagcaaa gatagatatg gatgaatggt
      961 cttttcatcg ctatgttatt tcgaatcctt ctgagtttac taagcttgag gttggctttg
     1021 ctgcgttttt cttaaataga acaaatcgtt caggtattct caaggcgggg gttataggcg
     1081 ggaaatcaca aaatggaaaa tggaaaatgg atgttcgctt taacagggcg gatttaattt
     1141 caagaattga gaagatagca aattataatc aaagaatagt agtaactaat ctggatgctt
     1201 tggattttct tg
//
LOCUS       DAZGAP010000444         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGAP010000444 DAZGAP010000000
VERSION     DAZGAP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.447"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHLAM_005404"
     CDS             complement(<1..1001)
                     /locus_tag="ACHLAM_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7856750.1"
                     /translation="MRSFLTSKADSIVQQLSGLSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPSRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLEADG
                     "
     gene            complement(986..>1212)
                     /locus_tag="ACHLAM_005405"
     CDS             complement(986..>1212)
                     /locus_tag="ACHLAM_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856751.1"
                     /translation="YDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKP
                     QALTLAWSDEDGIVLVFDAQAEGESSCAVS"
BASE COUNT          274 a          364 c          315 g          259 t
ORIGIN      
        1 ccgtccgctt caagtacctg acccggcgtc agataaggac tggaggaatg cccgctgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgc cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgtccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgctc aagacggctg
      421 tccatttcat aacgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagctcc cgggatggat actcccggga gagccggaac
      541 tcaaaatcct gcccttccag accatgttta cgcaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcaat acggggaacg
      661 atcgtcagtg cataacgggt ctcatcattc gaggcagaaa tgcgacgaaa cgactcaacc
      721 acgccgaata ccgttctgag cggcagggcc ggtgttccgt cagacaccgg ggtctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgatagccc tgaaagctgc
      961 tgtacaatgc tgtcagcctt gctggtcagg aaactgcgca tgacgactct ccctctgcct
     1021 gtgcatcaaa taccagaaca atgccatcct cgtcactcca tgccagagtc agcgcctgcg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gcggatgtca
     1201 gggcatcgta ca
//
LOCUS       DAZGAP010000445         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGAP010000445 DAZGAP010000000
VERSION     DAZGAP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.448"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHLAM_005406"
     CDS             complement(<1..1001)
                     /locus_tag="ACHLAM_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7856752.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTTGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(998..>1212)
                     /locus_tag="ACHLAM_005407"
     CDS             complement(998..>1212)
                     /locus_tag="ACHLAM_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856753.1"
                     /translation="YDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKP
                     QALTLTWSDEDGIVMVFDAQAEGEPS"
BASE COUNT          270 a          360 c          313 g          269 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccactgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgt cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgcccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgttc aagacggctg
      421 tccatttcat agcgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagttcc ctaggtggat actcccggga gagccggaat
      541 tcaaaatcct gcccttccag accatgttta cgtaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcgat acggggaaca
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcaaaga tacgacgaaa cgactcaacc
      721 acgccgaata ccgtcctgat cggcagggcc ggtgttccgt cagacatcgg gacctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaagctgc
      961 tgtacaacgc tgccagcctt gctggtcagg gaacggttca tgacggctct ccctctgcct
     1021 gtgcatcaaa taccatcaca ataccgtcct cgtcactcca tgtcagagtc agcgcctgtg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta ca
//
LOCUS       DAZGAP010000446         1198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGAP010000446 DAZGAP010000000
VERSION     DAZGAP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.449"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..476
                     /locus_tag="ACHLAM_005408"
     CDS             <1..476
                     /locus_tag="ACHLAM_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7856754.1"
                     /translation="PSEKSEGYHRVRKPNHYVRNITHSVIKNTYVLPEALTGQIREEE
                     AIMLTSQLQALPEELAQGVLESLSGMLGSHSLQNPVGWLLAVLKRAREGNFYPPKQKQ
                     RVVVPASVLAGERGRATAQSVSSGKRTEPISAERLSGIVAGLRASLRRCSAMRPE"
     gene            588..1067
                     /locus_tag="ACHLAM_005409"
     CDS             588..1067
                     /locus_tag="ACHLAM_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856755.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWITYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
BASE COUNT          333 a          242 c          342 g          281 t
ORIGIN      
        1 aaccatcaga aaaatcagaa ggttatcaca gagttcggaa accgaaccat tacgtacgta
       61 atatcacaca cagtgtgatt aaaaacacgt acgtattgcc tgaagcgctg acagggcaaa
      121 tcagggagga ggaggccatt atgctgacca gtcagttgca ggcgctgccg gaagaactgg
      181 cgcagggggt tctggaaagt ctgtcaggga tgctgggcag ccactcgctg cagaatcctg
      241 tgggctggtt gcttgcagtg ctgaagcgag cccgggaggg aaatttttat ccgccgaagc
      301 agaaacaacg tgttgttgtg ccagcctccg tactggcagg agaaaggggg cgagctactg
      361 cgcaatcagt ttcatcgggg aaacgtacgg aaccgatttc agcagaacgt ctgagtggca
      421 ttgtggctgg attacgggcc tcgctgcgca gatgttctgc gatgagacct gagtgattgc
      481 gatacgggct atgttcgcgg gaataaaaat caaccagtgc cgccagcgac tggttaaaaa
      541 gaaaacagaa tcgggaactg gtcaggtgaa acaaggtggt aaatagcatg cagaaataca
      601 aacaggaaag tctgagtgca gccatgaaag aaagtggcct gttgatcgct aaaatttgtg
      661 cctgcgtgat tgccggagtg gtggttatct ggggactggt gacaggcaca gagaccctgc
      721 tcactgcgct gaaaacaatg cacgaacagt acgataacag tatgtgttat atcgtatgtg
      781 ccacagggtg gtttttcctg gtgttaagta actggctgtg gatcacatac agtctttata
      841 aagcgcgtac aggcagtaca gtcggagggg gcctgcaatt tatgctcctc ctgagtctgc
      901 tggcagaatg cttttcattt tttgtgattt tctggtcatt gtctcatctg gatataaccg
      961 acattcagtt gctcgtgatg gggataacaa cattaatgct gtttttgtct ctgaacgtta
     1021 aggtagtagt catcattaac catgttgcca gtggcaacag tgattaaaaa gcgagcggtg
     1081 ttgccagtgg caacagagta cagaaaatag tcaatggtgc cagtggcacc agagtacaaa
     1141 aaatactcaa tggtgccagt ggcaccatag tataaaaaat agccaatggt gccagtgg
//
LOCUS       DAZGAP010000447         1193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGAP010000447 DAZGAP010000000
VERSION     DAZGAP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.450"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..204
                     /locus_tag="ACHLAM_005410"
     CDS             <1..204
                     /locus_tag="ACHLAM_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO7856756.1"
                     /translation="KVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEILTAI
                     KEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(240..1175)
                     /locus_tag="ACHLAM_005411"
     CDS             complement(240..1175)
                     /locus_tag="ACHLAM_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7856757.1"
                     /translation="MGFIMNKDKSLNEMMLSLFNKSTIVDLSSETISESKSLSGRIYQ
                     DPEHNGEFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYQAVMLDKEGTALAPE
                     FLSQGYTLFVLSYRLPSPAEEENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFS
                     AGGHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLG
                     DNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHL
                     YEKGGHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          421 a          246 c          187 g          339 t
ORIGIN      
        1 aaagtattaa aaacaataca ctcagcattt tctgaatggc tgataatggc caaacctctg
       61 gaaaagaata aaatgacata tgaaaataac aaaaaaaccc atcagcagca tattgaaata
      121 cttacggcca taaaagagca tgattgtgat aaagctgaac agctaatgta taatcacctt
      181 tataaacgaa ttgaagcatc atagttactc gacgcttcac caggtagtca ccataaatat
      241 tatttaaatc catgagaata catccagtcc ataaacagtt ttatccatcc tccaacagga
      301 agatctgtgg cccactgaac actaaagcca tgccctcctt tctcatagag atgcatttca
      361 gcagaaacgc ctttctctat taggccacgg tacatgagca gactgttttc aattgacaca
      421 agatcatcat cacaacaatg gagtaaaaac acaggaggaa aacatgatgt gacattaaga
      481 tccgcactat attgtattat attttctgag gtactattgt cccccagtaa ttgcttcctg
      541 gagcctggat gtgtcacatc atctttcatt gatattacag gataaattaa cccgacaaaa
      601 tcgggaatag cgaaaatctc ttgttcttta ctgtcagaag aacgtttgaa atcacaaatc
      661 tgattcacaa acgttgaggc gatatgcccc ccagcagaaa atcctaatac accaatacga
      721 tgacgctgta gatgccattt atcagcattc ttacgaatat aaagcattgc agatattgca
      781 tcaataaaag ttttgtttat attttcttcc tcagctggag atggtaatct gtaactaaga
      841 acaaataatg tatagccctg agataaaaac tctggagcaa gagctgtacc ttccttatct
      901 aacataacag cctggtagcc ccccccggga ataatcaata ttcctgttcc atttggttta
      961 tctggtaaaa aaataattaa ctgaggagca taaatatctg tcaatacacg atcaagttca
     1021 aattccccgt tatgttcagg atcctgataa atacggcctg aaagagattt actctcagat
     1081 attgtctcac ttgacaagtc aacaatagtt gatttattaa acaagcttaa catcatttca
     1141 tttaaagatt tatctttatt cattataaac cccattaaaa taactcaggt gac
//
LOCUS       DAZGAP010000448         1188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGAP010000448 DAZGAP010000000
VERSION     DAZGAP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.451"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..486
                     /locus_tag="ACHLAM_005412"
     CDS             52..486
                     /locus_tag="ACHLAM_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO7856758.1"
                     /translation="MNDNALVNVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            490..804
                     /locus_tag="ACHLAM_005413"
     CDS             490..804
                     /locus_tag="ACHLAM_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856759.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            888..>1188
                     /locus_tag="ACHLAM_005414"
     CDS             888..>1188
                     /locus_tag="ACHLAM_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7856760.1"
                     /translation="MSEWRKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIK"
BASE COUNT          304 a          252 c          344 g          288 t
ORIGIN      
        1 cctcacgggg cagtgctctc tgttatttca ggattacaga ggaactgagt gatgaacgat
       61 aacgcactgg ttaatgttga cgatgctgtt gtggcccgta tcagggatgt gctggcgtat
      121 acatttccgg gacagaaatt ttctgtgcgg aaatgcgatc catctttccc gaaagttttc
      181 aacaatgtgt gggtgagctg gctggatggt ccaacggagg ccgaagtacg ctctgtcaca
      241 gaggggtttg agaataaaga cgggctgctg tctgtgcttg aatcccgtta tttcagcgat
      301 gaactggtac aggagtgtat tgatcgcctt cgtaaagagt acggacaggg gaacgtcccg
      361 ttgagtgtga ccgtggagcg ttaccggaaa aacgagctgg agaagattga aacagcgcgt
      421 tttccgggca acatatccgt agccataaac gagatcggga taaataccag caaatacagg
      481 aggtaaggta tggctggtaa atgccttgtc tggttctgta acgggatctc tgaaccgtgt
      541 ctgatagcag gaaatatcag tgaattactg gcagatgtta cgatcacgtc aaattaccgt
      601 ggagatgtca catctgaaat cagggatgtt gtacaggagt ggctcaggac cggcaggggg
      661 aacctggcgg atttaaagga agagctctgg tactacaacc ttttcatcaa tccgtctgca
      721 gacgaactga tgaatgtcaa caggcggtat ggccctgagc gaactgcctg gctgcagggt
      781 tttatccgca acaacggggc gtgatacccc gttttcccgt gagggcattg tacctcacgg
      841 gacaatgccc tctttttaca tctgtataaa aaagaggagc gtaaacaatg tctgaatggc
      901 gcaaaaaccg ttttgaaatt accggtaaat cagtctgtct tgatgtactt acacagtgga
      961 ttgaagggcg tgagaccccg cgctaccgtc atgccataca gcagagtata cagctgtttc
     1021 tggccggttg tgcagggctg gtaaaaccca cacggacgac gcagtatccg ccatatccgg
     1081 cgctggttcg tcacggaacg gggttgtcat ctccggctaa ccaggcgttt gagcagtggc
     1141 tggggttgct ggtaaaaaat gccgttctgg atggtgaaac gataaaga
//
LOCUS       DAZGAP010000449         1175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGAP010000449 DAZGAP010000000
VERSION     DAZGAP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.452"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            413..606
                     /locus_tag="ACHLAM_005415"
                     /pseudo
     CDS             413..606
                     /locus_tag="ACHLAM_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            704..>1175
                     /locus_tag="ACHLAM_005416"
     CDS             704..>1175
                     /locus_tag="ACHLAM_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7856761.1"
                     /translation="MIVCYVILVNRPFETEIAGKKLFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDIS"
BASE COUNT          328 a          254 c          268 g          325 t
ORIGIN      
        1 ctcggcatgc cagttaacag ccatccgcaa tggcgtgttc ggttttgcga ataaaaaatg
       61 gttactgcag aagcggattt tcaccacctg tcaaccatga atggtcagtg gagtgagacg
      121 actggtatga tttggtcgat gacttcttcc ggtgaatacg gacagaaaca ttgaaatatt
      181 gtaagagaga acgttcagaa ttctgtgtca cgtgaaaata ctacagcgtt atcaccttat
      241 tcagtcgacc ttcaaaataa acgtctaacc gtgacaatca ccatttggat tgcccactgg
      301 ggaggcgaac gattgcctcg acggcattta taatgccagc ccacattgtt ggcgctgaca
      361 atactaagga caaacgatga gggagaaccg tcttatacca ctagcgctga caccggcaca
      421 ccttccccgt gaaatacacc ggctggagcc tgaagaaagc tgttgcccgg agtgtggcgg
      481 tgagctggat tatctgggga agtcagcgct gaacaactgg aactggtgag cagctccctg
      541 aaagtgatca gcacagaacg ggtaaaaaaa gcctgtaaaa aatgtgacgg taccgttgaa
      601 gcgccggtct cgtaagaata atcctggaca aataagattt ttcctgaaaa agcgtctagt
      661 cttttgatat cgttgttttt atgctaatca gggagtatgt gtgatgattg tctgttacgt
      721 tattcttgtt aaccgtccgt ttgaaacaga gattgccggg aaaaagctat tttttcagaa
      781 aaatgacgtc ctgttgacag ataacatcag ccagtccttt ttcctgcgcc atcccggtaa
      841 tgttactgag ctccggattg acgaatgcat tgtcagaaaa tatatggaaa gtgccgacag
      901 gatccagcac tctgatagtg ctgtttctgt tccgttcatt aaaacaacct tctctcacat
      961 cgctctgtta cataatgtca tgcagaatct cagtctgcaa aagacattgc cacaacaatt
     1021 ttctgagcag atgttttttg cctgcctttc cattttttcc acacacagag gtttcatgtt
     1081 tctgctattc agatgtataa actctgttgc gcttaaagtc aggtatatta tctgttccag
     1141 tccatcacgg gcatggagag ttaaagatat cagta
//
LOCUS       DAZGAP010000450         1168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGAP010000450 DAZGAP010000000
VERSION     DAZGAP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.453"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..402
                     /locus_tag="ACHLAM_005417"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHLAM_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            394..729
                     /locus_tag="ACHLAM_005418"
     CDS             394..729
                     /locus_tag="ACHLAM_005418"
                     /inference="COORDINATES: protein motif:HMM:NF026979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain fold toxin domain-containing protein"
                     /protein_id="HFO7856762.1"
                     /translation="MAKTRDLATTISDKFKQLFQCDKFAAALKSKMQQAGVNGEHLIL
                     ETKNKQGAFGNIWSDRIGKNIATNGHHEAIKVGDTVFDNMNPSGIKYEDWLNDIYSPG
                     EYTITPSRF"
     gene            732..1013
                     /locus_tag="ACHLAM_005419"
     CDS             732..1013
                     /locus_tag="ACHLAM_005419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856763.1"
                     /translation="MNDIVKLIDMIEKRPALYLSRHTISALKAFLDGWQMREPKEIVN
                     IEILIRLQSYIEDYYGVTGHSWDKIILLFSQDEHDALNTFFIRYNEIKK"
     gene            complement(1051..>1168)
                     /locus_tag="ACHLAM_005420"
     CDS             complement(1051..>1168)
                     /locus_tag="ACHLAM_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin SymE, type I toxin-antitoxin system family
                     protein"
                     /protein_id="HFO7856764.1"
                     /translation="KAALRDLSLVQELRKELYQVKQVVKGMKNGISGVLREV"
BASE COUNT          360 a          232 c          268 g          308 t
ORIGIN      
        1 ccgtcggtgg ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc
       61 cgtccgctga tgctctttaa cagtgaaggt aaaaccgtgt ggcgaccgtg acaaaccagc
      121 ctgtgggggc tggcactcag tctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gacccgatag ggctgagagg cgggataaac aattatgcat atgttcataa tccccttggt
      361 tatattgatc cattaggatt aaccatttgc gatatcgcaa aaacaaggga tcttgcaaca
      421 acaataagtg ataagtttaa acagcttttt caatgcgata aatttgccgc agcgttaaaa
      481 agtaaaatgc aacaagctgg ggtaaatggt gagcatctca tactcgaaac taaaaataaa
      541 caaggtgcct ttggcaatat atggagtgat agaataggta aaaatatagc aactaatggt
      601 catcatgaag caataaaagt aggtgataca gtttttgata atatgaatcc ttctggtata
      661 aagtatgaag attggctgaa tgatatatac tctcctggcg aatacaccat tactccatca
      721 aggttttaat tatgaatgat attgttaaac tgattgatat gatagaaaaa agaccagctt
      781 tatatttaag caggcatacc atatcggcgt taaaagcttt cctggatgga tggcaaatga
      841 gagagccaaa agaaatcgtc aatattgaga tattaataag gcttcaatct tatattgaag
      901 actattatgg agtaacagga cattcatggg ataaaattat tttattgttc tcacaagatg
      961 aacatgatgc gctaaatact ttttttatca gatataatga aataaaaaaa taatatgttg
     1021 ccgggagtga caatctcccg gcttttattt ttatacctcg cgtaatacgc cggatatccc
     1081 gttcttcatt cccttcacca cctgtttaac ctgataaagt tccttcctca gttcctgcac
     1141 cagggaaaga tcacgtaacg ctgctttt
//
LOCUS       DAZGAP010000451         1157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGAP010000451 DAZGAP010000000
VERSION     DAZGAP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.454"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..>1157
                     /locus_tag="ACHLAM_005421"
     CDS             118..>1157
                     /locus_tag="ACHLAM_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7856765.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKR"
BASE COUNT          394 a          176 c          249 g          338 t
ORIGIN      
        1 cgattattgt tgtatattca agataatgtg accttaattg tctttttaaa taaaaattaa
       61 acaaaaatta tatcccacca ctaaggttta taaaagcata cgttagcagg tgtcaccatg
      121 aaaaaagcca tagcatatat gcgattttca tcaccaggtc agatgtctgg cgactcatta
      181 aaccgacaga gaagacttat tgctgaatgg ttaaaggtaa atagtgatta ttatcttgat
      241 accataacat atgaagattt aggattaagt gcattcaaag gaaagcatgc acaatcagga
      301 gctttttcgg aatttttaga tgctatagag catggttata tattgccagg aactacattg
      361 ttagttgaaa gtctggacag actttcaaga gaaaaagtcg gtgaagcgat tgaacgtctg
      421 aaattgattt tgaatcacgg tattgatgtt ataactcttt gcgataatac agtctataat
      481 attgactctt tgaatgagcc atattcatta ataaaagcca tacttatagc acaaagggca
      541 aatgaagaaa gcgagataaa gtcaagtcgg gttaaattat catggaagaa aaaacggcag
      601 gatgcactgg aatcaggtac gattatgacg gcgtcttgtc cgagatggct ctccttagat
      661 gacaaaagaa cggcttttgt tccagacccc gacagggtga aaactattga gctaattttt
      721 aaactcagga tggaaaggcg ctcattgaat gcaatagcca agtatttaaa tgatcatgct
      781 gtaaagaatt tctcaggaaa agaaagtgca tggggacctt ctgtaattga aaaattatta
      841 gcgaataaag ctctgatagg tatatgcgta ccttcatatc gtgcaagagg gaaagggata
      901 agtgaaatcg ctggctatta tcccagagtc atatcagatg atttgtttta cgctgtacag
      961 gaaattcggt tggcaccttt tggtattagc aatagtagca agaatcctat gctaataaat
     1021 ctacttcgaa cagttatgaa gtgtgaggct tgtggtaata ccatgattgt tcatgcggta
     1081 tctggaagtt tgcatggcta ttatgtttgt ccgatgagaa gattacatcg atgtgacagg
     1141 ccatcaataa aaagaga
//
LOCUS       DAZGAP010000452         1155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGAP010000452 DAZGAP010000000
VERSION     DAZGAP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.455"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..602
                     /locus_tag="ACHLAM_005422"
     CDS             <1..602
                     /locus_tag="ACHLAM_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO7856766.1"
                     /translation="TQPSPAEQQLRALLAPLPDMLSADQMAQLSRTPSLLNHASDWIT
                     LSGQQITRLTELPLTYNLQRSAQLLQQLMVLFPDNPRVQEMVDNWQKSVRSRALPEEA
                     MTGWNEGMTRLQQLAERLNRLDEQRGKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRY
                     DEVRNQNGSESQQKLAQDALMEQLNRYWLLRHGDAGNPA"
     gene            584..808
                     /locus_tag="ACHLAM_005423"
                     /pseudo
     CDS             584..808
                     /locus_tag="ACHLAM_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            811..1097
                     /locus_tag="ACHLAM_005424"
                     /pseudo
     CDS             811..1097
                     /locus_tag="ACHLAM_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          326 a          242 c          314 g          273 t
ORIGIN      
        1 tgacacagcc atcaccggcg gagcagcaac tcagggcgct gctggcacca ctaccggata
       61 tgctttcagc agaccagatg gcacagctca gcagaacacc ctcactgtta aatcatgcgt
      121 cagactggat aacactttca ggtcagcaga taacgcgact gacagaacta ccgctgacat
      181 acaacctgca gcgtagtgcg cagctgttgc agcaactgat ggtacttttc cccgataatc
      241 ctcgtgtaca ggaaatggtg gataactggc agaagagcgt aaggagtcgt gcgctcccgg
      301 aagaggcgat gacgggctgg aatgaaggca tgacccgctt acagcagctg gctgaacgcc
      361 tgaaccgcct ggatgaacag cggggaaaat acatgacggt cagtgaactg aaaacggagg
      421 tgtttggcat catgcaggct tttaaccggc acataccggc agaagagcag ttacgtcgct
      481 atgatgaagt ccgtaatcag aatggcagcg aatctcagca gaaactggcg caggatgctc
      541 tgatggaaca gttgaaccgc tactggcttc tgagacatgg agatgctggg aatccggcgt
      601 gacttgggtt cgccaaccac cggaagacgc cattacggat atggcgtatt ttccctttta
      661 ccggtatgcc atggcctttg aaggattcat tccggcattt tcatcttggt ttgcaggtaa
      721 ccggtgtgga gtggcaatac tgaccgatgt ccgggcggat gaatccctca actgctttat
      781 ggggctggtg tctcagcgca aactgagaga attacgtggg taggatcaga tgatggtatt
      841 ggaactgaag gtgatatggg aaaatggttg ttattatttt gtctgctgta gctcagtctg
      901 aatgacaaca tatacttgaa agaacaaatg aaggccgtct cgaagccgaa gtaaagggta
      961 tttaatttgg gcgaaaaaga acggtcaaca gggaacaagt acaaacctta cgaagtctgg
     1021 gtatcggcgc tacagaaatt tcgcgaaaga tgaaatttac acgttctact gtgtataaaa
     1081 tattagaggg taaataatat tttcatatgg agggttacat gaaacgtatt tcagtaaatg
     1141 ttttagattt atatt
//
LOCUS       DAZGAP010000453         1148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGAP010000453 DAZGAP010000000
VERSION     DAZGAP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.456"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            513..1064
                     /gene="ybcL"
                     /locus_tag="ACHLAM_005425"
     CDS             513..1064
                     /gene="ybcL"
                     /locus_tag="ACHLAM_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO7856767.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            1074..>1148
                     /locus_tag="ACHLAM_005426"
     CDS             1074..>1148
                     /locus_tag="ACHLAM_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856768.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRV"
BASE COUNT          360 a          175 c          261 g          352 t
ORIGIN      
        1 gcgataagtg ctctcaaata tatggttgat ggtgagattt atttctaaat aatgatctcg
       61 gattttaagt tatgctatgg tgataaagtg caagacagaa ttaattatct ttgacgaaac
      121 ttaatgggta attactttgt ttgctcccac aagcgagttt tgtacggctg tattggggta
      181 gtaaatgagc tatacaatct taatcatttg ttaggtgaga actcttggtc gcagattcaa
      241 atactgaaaa tacgtgacaa attattatga gcaaaatggt gtatgtcacg tattttgaat
      301 ggtaggttaa aaaataacac cgactttcgt aggtattact aataataaag cagagttttt
      361 agatagtatc aatgtgcttt gtgtatattg tggcaaataa ttgggttggg ggtacaattg
      421 tgattgcttt tgcatgaaca ttgcgccttt atgcataatg agataaagga atatcaaata
      481 aaataacgat aggtcataac aaagaggttt ttatgaaaac acttatcgtt tcaactgtat
      541 tggcattcat aacattttct gcgcaggctg cagcatttca ggtcactagt aatgaaataa
      601 aaacaggaga gcaacttaca acgtctcatg tcttttctgg atttgggtgt gaaggtggta
      661 atacatcgcc ctcattaacc tggtctggtg ttcctgaagg taccaaaagc tttgccgtaa
      721 ctgtatatga tccagatgca cctacaggca gtggttggtg gcattggact gttgttaata
      781 ttccagcaac agtaacatat ttgcccgttg atgcagggag acgtgatgga acaaaactgc
      841 cgactggtgc tgttcaaggc cgaaatgatt ttggctatgc tgggtttggt ggcgcatgtc
      901 ctcctaaagg agataaacca catcattacc agtttaaagt atgggctcta aaaactgaaa
      961 agattcctgt agattctaac tccagcggag cgttagttgg ttatatgctt aatgctaata
     1021 aaatcgcaac cgctgagata acaccagttt atgagataaa gtagggtgag agtatgctgg
     1081 caagaggtaa gactaactta aagatcgaag aaatacggat gcataaacat catgagattc
     1141 atagggtt
//
LOCUS       DAZGAP010000454         1135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGAP010000454 DAZGAP010000000
VERSION     DAZGAP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.457"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            8..718
                     /locus_tag="ACHLAM_005427"
     CDS             8..718
                     /locus_tag="ACHLAM_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO7856769.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDVNKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATQDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            730..>1135
                     /locus_tag="ACHLAM_005428"
     CDS             730..>1135
                     /locus_tag="ACHLAM_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFO7856770.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDITKDKWEKLMTRPPVSLAGHVSF"
BASE COUNT          389 a          176 c          242 g          328 t
ORIGIN      
        1 ggcatttatg aaaaaatatt atccgctatt aactgtaatt cttcttggtt tacctttgtg
       61 ctctcaggca ttaactctta atctacgtgg gggatacaga agcgccagtc acgcttatga
      121 aacgttgatt caggttagtc aggggtggaa taatggttgg tgggcaagtt ttgagtcaaa
      181 cacctggaat acaattcaca acaatgaaaa tgaattgttt gctctcaatt ataataaaat
      241 agatgtaaat aaaacatata aaatagatga caaatgggct ctaatcccag ggattcagac
      301 acaatggatg tcagcaggaa gtcagtataa cccttatctg aaaatttcgt atcaggcaac
      361 gcaagatctt aatctggcct tacgctatcg ctatgactgg aaagctttcc gacagacgga
      421 tttagatggc aacagggcgc gtaatgatca gcatcagttt gatggttatg tgacatacaa
      481 aattaatgat tcatggttgt ttgcatggca gaccacggta tatacgaagg ttaataattt
      541 caaatatggg aatcataaaa aaacagcaac tgaaaatgca tttgttctgc aatataaaat
      601 gtcaccggtg tttaccccat atatagaata tgattacctt gataaacaag gggtgtataa
      661 ggggaaagat aataaacatg aaaatagtta ccgtgtcggt gttctgatta atttttaaag
      721 gtgattacaa tgaaagtgaa aaactttata tatcttccct tctgcttgtt tattggaacg
      781 tcggtagccg gagctttacc ggaaattcct gaaccattta aatatggtgt aggagggatc
      841 gagaatggta aaatatatat aggactggga agtctgggaa ataactggta tatgatagat
      901 acaaaccaga gtgaaaaaaa atggactaag atagcacaat ggccgacagt tcccagagag
      961 caggcaacag ctactattat tgatggtaag atctatgtat ttggaggaat tggaaaagat
     1021 aaaagtggag tcataacttt acaaaaagat gtctattcct atgatattac taaagataaa
     1081 tgggaaaaat tgatgacccg tccccctgta tctcttgccg gtcatgtttc tttta
//
LOCUS       DAZGAP010000455         1133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGAP010000455 DAZGAP010000000
VERSION     DAZGAP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.458"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1133
                     /gene="espI"
                     /locus_tag="ACHLAM_005429"
     CDS             <1..>1133
                     /gene="espI"
                     /locus_tag="ACHLAM_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFO7856771.1"
                     /translation="ALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFSLSR
                     NAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHITLEQ
                     KSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGLFTS
                     GSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGDIIS
                     DKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIHAPSASATMNNTWWQL
                     TGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLVVNQ
                     KLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQA"
BASE COUNT          322 a          240 c          280 g          291 t
ORIGIN      
        1 gctcttatct tccagggaca cccggttatt catgcagggc aaactgtttc tgcatcgcag
       61 agtgactggg agaacaggga gttctcactc aacaatctga atcttaataa tgcggacttc
      121 agtctgtccc gtaatgcatt tatgaacggg aatatcaggg ccgttaacca gagcactgtt
      181 attatcggcg gagatacagt ctttactgat aaaaatgacg gaacaggtaa tgatgtcatc
      241 agtgttgaag ggaaatctgc tgccgcagga acatcctcct atacagggca tatcactctg
      301 gagcaaaaat cagcactgga tatccgcgat aattttcgtg gcggggttac gtctgaagac
      361 agtcatatca atgtttcttc atcttcagtc ctgttctcag atgcatcgtc atttataaac
      421 agctccctga atattcataa aggaggtgcg ctgaccgctc agggagggct gtttacaagt
      481 ggaagcattg atattggtga cgcttccctt ctgcttaccg gtacaccagt gaattcagat
      541 gatgctgctt ttttaccgac catcaatatg gctgatggcg gatttaaact gatgtctgat
      601 tcatcagtac tgaaagccag agaccaggca tctgttgttg gtgatattat ttctgataaa
      661 caggccacaa tcagcttcgg aactgaatca ggtaaagagg gcatattgtc tgagaaggca
      721 tcccggggac tcgcggtagg attactgagt ggttttaata cggcataccg cggtgcaatt
      781 catgccccgt cagcatctgc cactatgaac aacacctggt ggcaattgac aggagactcc
      841 tcacttcgct cgttaaaaaa taccggaagc atgacatatt ttacaggaag tgcagcgaat
      901 aaagcattcc atacactgac ggttgatgag ctgacgacga atggcactgc gtatgccatg
      961 cgtacggacc tgaaaaatgc ggataagctg gtagtaaacc aaaagctgtc aggtaaggac
     1021 aatattctgc tggttgattt tctgaacaaa cccaccggag aaaaactgga tattgaactg
     1081 gtgagtgcac cggggaacag cagtaaggat gtttttaaag gaagtgaaca ggc
//
LOCUS       DAZGAP010000456         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGAP010000456 DAZGAP010000000
VERSION     DAZGAP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.459"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(414..989)
                     /gene="dolP"
                     /locus_tag="ACHLAM_005430"
     CDS             complement(414..989)
                     /gene="dolP"
                     /locus_tag="ACHLAM_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7856772.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSYIR"
BASE COUNT          298 a          302 c          248 g          284 t
ORIGIN      
        1 ttgtacctcc tgaatacact gtacggcaaa ttgccgtatt gtgcaaatta cactctgaca
       61 ccaggacggc agacaggcat tctgcctgaa aagaaaaatc agattcagac aaaactatcc
      121 gggggatgtt aacgccacga tctttgacca ttgcatacag ataaaagctc agattattta
      181 ccagcagcga catccggagt acaccgggat tttatctgtg agtgaacata cccgggcaaa
      241 aaaatccccc gaattcaggg ggtgtcaaag aggaattatg tatattggtt attataatga
      301 gcacgtctcc ggtattccgc gacgaatgtt cacgtggtgg catgaacggc ccggaaacgt
      361 gctcttctga tttttaacgt aaatttgcac taaaggcaac tcagatcctg ccttcaccgg
      421 atataggaga atgccgtcgt gacccgcttc acgccactca ccctactggc aacatctgct
      481 gccgctcgcc cttcctgctc tgtaaccagg cccagtaaaa agacttcgct gttttctgtc
      541 accaccttga cactggatga tttgacacgg gcacttgtga gtaactggga acgaattttt
      601 gtcgtgagcc aggcatcacc agccattatg accggactga cgggagtccc ctgccggatt
      661 tcattataga cctcctgaac cccatcgaca ccgagtgcta tctgacgggc acgggcagat
      721 aattcacggt ccggagactg accgatcagc agcacgcgtc cctgatatgc aatctcactg
      781 atgcgtgctt cttttctgat ttgttcatct tttgaaagtg cactgctgac cctcagctcc
      841 agcacttcat catccacctg cgttccgaca ctgcgtgggt cggttgctgc ctttgtcccg
      901 acagcggctg tacccaccac agttgcagca acgcatccct gtaacaataa ggctgaaatc
      961 aggacaacag cagaatataa taaacgcata tctcccccgg aattgttttg atttttactt
     1021 catattcccg gaatttatcg tacaaaaagt ttaccttcag tgaaacaaaa caagctacag
     1081 actgttatta tcctccggca cagccggaaa tagcggatgc gttacgtggc at
//
LOCUS       DAZGAP010000457         1130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGAP010000457 DAZGAP010000000
VERSION     DAZGAP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.460"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..830)
                     /gene="yegS"
                     /locus_tag="ACHLAM_005431"
     CDS             complement(<1..830)
                     /gene="yegS"
                     /locus_tag="ACHLAM_005431"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO7856773.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     "
BASE COUNT          306 a          280 c          252 g          292 t
ORIGIN      
        1 ttttgcccac tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata
       61 tcaaaccacg acgaagctcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt
      121 gatacgagag tcggaataat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg
      181 ttaattaacg cgttcgggca caattgttga ccgccaccgg cctgacgccc gttaccaata
      241 ccaatgacca gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc
      301 ggttgcagag tgtccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg
      361 gcttttaatt tttccggcgt ttctgtggta atacgcgtcc caaacccgcc tgtcgccata
      421 ttaataaaac aggtttgttt attgacctgc gccatatcta tcgcaatggc gttaccggca
      481 attgccagtt tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca
      541 ttggcggttc ctaatggtaa aattcccagc gcgggtatgt catccccctc acactgaatc
      601 aacgccgtag aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg
      661 cccaacttcc gtgcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc
      721 acatggatcg tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg
      781 tcagtacttt tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt
      841 gcctttatga ttggactgta gatagtgtag agcagaaaag aaaaggtgga gtcagaacag
      901 gatgaaagtc ggaggataaa aagtcagttc aagtatggag gattcagtgg ttaccaccag
      961 gagcttgcaa tgggaacagc gaatattaac ggtagccacc ggtaaatttc aaaaaagaac
     1021 atatacctaa tattcaacta aacagtggca tcttcaatat actattaaaa atatattaac
     1081 ccccccatgg ggttacctgg aagggcatcc gtgtatgtaa ttcctactta
//
LOCUS       DAZGAP010000458         1126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGAP010000458 DAZGAP010000000
VERSION     DAZGAP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.461"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..903
                     /locus_tag="ACHLAM_005432"
     CDS             <1..903
                     /locus_tag="ACHLAM_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO7856774.1"
                     /translation="LELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATL
                     KEIKDLAKNITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLC
                     KSASRMDQNLRAPVPMTWAFMMREAGFPAVRPKYELPLSKDVLDEVREEMRSLGSYIA
                     LNLEGSSQERTFSLSIAENLIEKIQSETDIPIVIVHGPKGEEKARVLVDCYNNVFRLS
                     LSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVV
                     VGQKIDNISLDEFAKALKSVLLRI"
BASE COUNT          365 a          207 c          246 g          308 t
ORIGIN      
        1 cttgaattgc ataaaattaa gcatctttta attgtaacgt cgacaattaa tttagaagtc
       61 tttaatgcgc tcaaacttga acagactaaa ttaacattag ttacaatgac tatgcaggat
      121 cacgcaacat taaaagaaat aaaagatcta gcgaagaaca taacacagca atacggtacg
      181 ccggatcttt gtatagaggc gatgcgtaaa aagaacctga aaacgatgat atttatcagt
      241 cagttgaaag caaaaacgaa ttttcaggtt gttggtttaa ccatgaagtg ctattcccct
      301 ttgtgtaaga gcgcgtcccg gatggatcag aatctccggg ctcctgtacc catgacatgg
      361 gcatttatga tgcgtgaggc gggttttcca gcagtcaggc caaaatatga attgccacta
      421 agtaaggatg tactcgatga ggtgcgcgag gaaatgcgat cgttaggatc ttacattgcg
      481 cttaatttag aaggtagctc gcaggaacgt acattttcat tatcgattgc agaaaatcta
      541 atagaaaaaa ttcaaagtga aacagatata ccaatagtga tcgttcatgg accaaaaggc
      601 gaagaaaaag ccagggtgtt agtggattgt tataataatg tcttccgttt atccttatca
      661 ccctcgatta aacgttcggc agcaatcata aaagatgctt atatcgcaat aactcctgac
      721 acctcaatat tacatatggc aagtgcctat aatacccccg ttgttgcaat ttatgctgat
      781 tacaaaacgc gatggcccgc aatggccgat gtctcggagt cagtcgtcgt tgggcaaaaa
      841 attgacaata taagtctgga tgaattcgca aaggcattaa aaagtgtttt gttgagaata
      901 tgaggcagta cttatgcata gtagtgccga tctgttatga acggtggtgt catcaatact
      961 ttgtggctgg tcaatcatta cagaaagcca gcctttggat caaagctgta ctcaccggat
     1021 acaaccatag tcacaccgaa cgcgaatctg gcggtatcgc atttttattt attcattttc
     1081 tgtgagagaa gggcaagttc tgtagagaga atgtaaaaaa gagcgc
//
LOCUS       DAZGAP010000459         1119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGAP010000459 DAZGAP010000000
VERSION     DAZGAP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.462"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1077)
                     /locus_tag="ACHLAM_005433"
     CDS             complement(<1..1077)
                     /locus_tag="ACHLAM_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7856775.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFT"
BASE COUNT          325 a          273 c          202 g          319 t
ORIGIN      
        1 ggtaaattcg ctttcatcaa taaggttaat tttgtaatca ataagaagtg acatgttttt
       61 gttcagaaag tagtttgcgc caacatcaat atactcaaca agagactgat cgccatagtt
      121 tactattcca cctttaacat ccatcccttt ggactgaacg taagccagtg aaggacgcag
      181 gccgaaatca aacatatact gagccacaac ttcaatattc cgtgctttat ctgcgatgcc
      241 tgcgctacca aatggagtca tatttcgggt ttcagagtag tcagctgcca gatacaggtc
      301 attatcatta tacttgaccc caacacctgc aaactccgca tgttttcccc cggcaatcca
      361 gcgaggcgta ataacttctt ttccttctga atctttcgat gtcatgaaac gtttacggcc
      421 ttctttttcc tgcagactgg ttctgtcggc attagtgtaa gcggcaacga ttccaaaacc
      481 ttcatagtca tagcttacgg agaaaccata tccatcacca ttagcttcaa gtaaattatc
      541 acggtcattt ttaccctgat attgagcaga aaaagtaagt ccatcaacaa gcccgaagaa
      601 atctgtattg cgccaggtca gaactccggt tgttctgccg gtcatatagt tatcagagta
      661 agtccagctg tcaccaccaa agtccggaag tacgtcagtc agagccccca catcataggc
      721 aacaccatag tttcgcccat aatcaagtga accgagtata ttgtggcgaa tacctgcaaa
      781 tgccagacga tttttatcgt tgcgtacttc actttctgtc ttaaacccca ggaactggta
      841 ttcccactga ccaaagccag tcaggctctc attaatttgt gtttcgcctt tgaaaccaag
      901 acgtgcaaat gttctgtcgc catccataga accatcagag atgtaatgac gggctttaac
      961 gcgaccataa aaatccagtt tattaccgtc tttattatag atttctgctg cctgtgttgc
     1021 ctcacacagc attaatgcag aaacgacaga gcacacaacc atacttagtt ttcgcataaa
     1081 aaaatcccct gtgcacattt ttagtatggt tttaaaaat
//
LOCUS       DAZGAP010000460         1116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGAP010000460 DAZGAP010000000
VERSION     DAZGAP010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.463"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1045)
                     /locus_tag="ACHLAM_005434"
     CDS             complement(<1..1045)
                     /locus_tag="ACHLAM_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7856776.1"
                     /translation="MLKDGTGTAVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAATPKAVKAA
                     MDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVAWLKEKGA
                     FDQPYWMMKASLPAVLNKVITDVGPGKLNLGGCVIEVMGTYNAAIVRVTIGEYGATGF
                     INGTVCTCTVYGDT"
BASE COUNT          181 a          401 c          236 g          298 t
ORIGIN      
        1 gtgtgtcgcc gtaaaccgta caggtacaga ccgtgccatt aataaacccc gttgcaccgt
       61 attcgccaat ggtgacccgg actatggctg cgttatacgt ccccatcacc tcaatgacac
      121 agccacccag attgagtttt cccggtccga catccgtgat gactttattg agtaccgcag
      181 gcagtgatgc cttcatcatc cagtaaggct ggtcaaatgc ccctttttct ttcagccagg
      241 cgacaaactc gctcgtggtc cattcaccgg cccccgtatg aatgtcccgc ccgtatactc
      301 tggctgctcc cacggtgttc agaaacttca ccttatccgg gatatcgtca ccgtttttcg
      361 ctttctccag gcatccgtcc gctttgtcca ttgccgcttt caccgctttc ggggtggctg
      421 ccttcgtttc atcatcactg tccgttgcgc tgcttaactg cacaattccc ttctgtgccg
      481 tcgtcgcatc cggtcctccc ggctcgcctt tttcgccctt ctctccccgt tcacctttca
      541 caccctgaag cccctgtggt cctgtctcac cacgctcacc ctttggcccc cgctcgccgg
      601 tatcgccttt tggcccggga ataccctgcg gcccggtgtc tcccttatcc cccttcggtc
      661 cccgcgcatt ctctgcccgt ttcttcgctt cctctgcact ggccgctgac gcttctgcac
      721 gtttcaggat ttccgctgcc accgcttcca gctctgcaag ggctttcggg taatactgtg
      781 cgtcctccag gtccatcaga aatttattca gcgttcccgg tgcagaatcc gccttcacca
      841 gaatgtcacc cacatatgac ggcgcgtacc cttccgtgtt cagcgtcacc cggtacaacc
      901 ccggctcaac atccatactg taactgccgg tttcccccgg ctgaccatac gccaccgtgg
      961 tgacaatcac cgtctccgtt gtgcggcgcg ctttcagctc tatcgtgcat cccggtaccg
     1021 ctgttcccgt accatccttc agcacacccg atattctgac tgtcatggat ttcccccata
     1081 aaaaaaccgc agtaccggtt tcccggcctg cggtaa
//
LOCUS       DAZGAP010000461         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGAP010000461 DAZGAP010000000
VERSION     DAZGAP010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.464"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          264 a          257 c          306 g          285 t
ORIGIN      
        1 agaaaaaagt tggttttgcg tggggggtgg gcatccgggg catgagaaca gactcgcgtt
       61 tttctggaga aactgcgggg atttttgatt aaacaatact caccgcagag cggaatttta
      121 tgcaacgccg gctgtgcggc acagggattt ttaatcccca gcatgtcaga gggtgcggat
      181 gacggttccc gtctggcgtt ttcatgggcg ccggttgtca aaaaatacca gcgtgatcgg
      241 tcgaaggata agcgcagatt aatcagtgat gattgagcgc gaatctgaca acaagtatag
      301 cgacacagca acaaaaaaga cgccagaaac gcgctcagag gctttgtaac gcgatttttt
      361 acatgaggca atggtttcgc tttgaggcgt tcagccgtgt tcagggcgcg attctggtac
      421 atacctgact gtaatcatcc ctacgaacta ctctttgcac cagaaacgcg caggagtgtt
      481 ttagcgtgtc tccggcgtga tgtcgagttg tgcggtaatg agtgagcgaa aaaaatcccc
      541 tggattacag ggggaaccca tgatcagact ttgaagttta tttgttattt gatggaatga
      601 gcacgaatcc cggagatgac gttcaggtgg caggaaacgc gctcagaagc tttctaacgc
      661 acttttttgc atctggcaat gggtttcata tgttttggtg ggttcaggct gtcagagacg
      721 ttttgagcgc gtttctggcg cttgctgcta ctgccaaacc atccccatgg ccagttgatg
      781 ccagaaacgc gcaggaattt tgtagcgcgt ctccggtccc tgatcaatga attccctgtc
      841 attaacatgc gtttacccgg aaacgcgccc tgatgttcta tcgtatcgcg ggaacggtgt
      901 cgagggtgtc ggggcaacgc cctgaccagg tggtaatcgt atagccgtgc gtgcgcggtt
      961 atacgattac acatcctgtc ccggaatcag gattgttttt cgcgcacaac tgcggcaaat
     1021 tcatcccgtt gcggttgcaa cgatgggtat atcaaaacta cgaaaattat tcaggcattt
     1081 gctacccccc ttgccctggc ctccaccagt ca
//
LOCUS       DAZGAP010000462         1110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGAP010000462 DAZGAP010000000
VERSION     DAZGAP010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.465"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1110
                     /locus_tag="ACHLAM_005435"
     CDS             <1..>1110
                     /locus_tag="ACHLAM_005435"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFO7856777.1"
                     /translation="NDCVILGGFIGAVVPECIEKGIEDRFLKTGKPRNLKIIFAAGQG
                     DAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGKITGYNLPQGVICHLFRDSAA
                     GKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEINGVENLFYKKIDANIAILRG
                     TTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQVKQIVRDGTLDPLQVRIPGIY
                     VDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETRPLDIKKIIARRAAMELKKNA
                     VVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGTPAGGLSFGASSFPQAIITQDQ
                     MFDFYDGGGLDQAFLGLAETDEKGDLNVSKFGTKIA"
BASE COUNT          350 a          166 c          274 g          320 t
ORIGIN      
        1 taatgattgt gttattcttg gcggatttat tggtgcagtt gttcctgagt gtatagaaaa
       61 aggcatagaa gatcgttttc tgaaaacggg aaaaccaaga aatctcaaga ttatttttgc
      121 tgctggtcaa ggggatgcag gaacaaaagc tgtcaatcat ttatctcatg aggggcttgt
      181 cactagtgcc attggcgggc attggggact aatacctaaa ttgcagaagt tagctaatga
      241 gggaaaaatt acgggttata atcttcctca aggggttatt tgccatttat tccgagatag
      301 tgctgctggg aagttaggga cattaactca tgttggttta ggtacatttg ttgatccccg
      361 cgtagagggc gggaaaatta atgataaaac acaagaggat attgtgtccg tcacagaaat
      421 taatggtgtg gaaaacctat tttataagaa gattgatgct aacatagcaa tcctcagagg
      481 tactacatct gatcagagtg ggaatataac aatggaggat gaatgtttga ttcttgaaaa
      541 cctggctgct gcccagttag tccataataa tggagggaaa gttattgttc aggtaaaaca
      601 aatagtacgt gacggtacat tagatccttt acaagtcaga attccaggta tttatgttga
      661 tgctgttgtt gtttctgaag atgaatatca tatgcaaact tttgcggagt atatgaactt
      721 cagctatgtc gggaaaggtg taaaagaggt aaaagacaaa gaaacacgcc ctcttgatat
      781 aaaaaaaatt atagctcgac gagccgcaat ggaactgaaa aaaaatgcag ttgtaaatta
      841 tggcataggg gcacctgaag ttatagctga agtaacagat gaagagggga ttacctccga
      901 acttatcgca actgttgaac caggtgcaat tggaggaacg cctgctggag gtctgtcatt
      961 tggagcttct tcgttccctc aggcgataat tactcaggat cagatgtttg atttctatga
     1021 cggtggtggg cttgatcaag cgtttcttgg attagctgaa acagatgaaa aaggagattt
     1081 aaatgtttct aaatttggaa caaaaattgc
//
LOCUS       DAZGAP010000463         1091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGAP010000463 DAZGAP010000000
VERSION     DAZGAP010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.466"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            644..>1091
                     /locus_tag="ACHLAM_005436"
     CDS             644..>1091
                     /locus_tag="ACHLAM_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856778.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPIFFSHGSHIHSYFICSP"
BASE COUNT          360 a          228 c          201 g          302 t
ORIGIN      
        1 caccggatag ataaggatca cacaaccact gctgtgtatt gcccgtaata aagggcgcgg
       61 ttaaaagaaa ccttcatcat catgactgac gatatctgct ctcaaaggtg ggcagattta
      121 ccaccaccag aaacatccta gctgcacatc ccctttcagg ttgcgggggt gttgatcacc
      181 cgaaggcgtt cctctcagat caacggaaca ttggtgccca ccgaagcggg atgtggtcac
      241 ccgaaggcgc tcctctcaga ccacttatgg agcattggtg gtcgccgaag cgacagataa
      301 tgcattctga gcatatcaga gcgcataaaa ggcgtcaatt atccatgctc cctcacgaaa
      361 cagaagctga actcttaaca cctgctatcg gaactcactg aataaataac taaaaaggac
      421 attgtgaaaa ttaacatcaa cagtatgtaa agtgatttgt tttgtcttaa atgggtgaat
      481 gtaaaaaaat attctataca atccacacat ccttgtatca tatatcaagt aaacatccac
      541 agagataaca gcttatttca aataagcaga ttctgtttga tacttcagaa aaatagtata
      601 tgctcctgat ttactgatgt tataagttat acaggatagt taaatgtgtg attttataaa
      661 atcatgttat gcagcaccaa gaattaacaa aatggctgat tttttctcat caacagcatc
      721 tggagtaaaa cataaacctt tgaagcatgt aaaaattgat aacaggcttt tcccatctcc
      781 tgagtatata attttttctg actatcttac gaaaaacagt ggtccatttg ttaaccacta
      841 cttttgcagt attccataca gcaaagaaga agaatgcagg ctgggtgtga ctattatgaa
      901 atatgcaagc catcgaaata agccattgag cttatggtgt cttggaatgg cagaaggagc
      961 catggccaga acaatttctc aactatcaaa aggacaggtt ttatcattaa caaccagccc
     1021 aacaaaagaa aatgaaccta tattctttag tcatggttct catattcact cttactttat
     1081 ttgctcccca t
//
LOCUS       DAZGAP010000464         1077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGAP010000464 DAZGAP010000000
VERSION     DAZGAP010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.467"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..594
                     /locus_tag="ACHLAM_005437"
     CDS             <1..594
                     /locus_tag="ACHLAM_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7856779.1"
                     /translation="YYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMG
                     LASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRAL
                     SPYDYTLYCRKWVKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEP
                     YLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            591..>1077
                     /locus_tag="ACHLAM_005438"
     CDS             591..>1077
                     /locus_tag="ACHLAM_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856780.1"
                     /translation="MKRILFFLLMIFVSFGVIADCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTIWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RM"
BASE COUNT          359 a          209 c          231 g          278 t
ORIGIN      
        1 tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatcct
       61 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt tcagtatata
      121 gattcaatgg gactggcatc aaaatatgga cacttaaata atggcggata tggagcgaga
      181 cccaacaaac cgcctacgcc cgatccaagt aaattgccag acatagcgaa acaattaaga
      241 ctgccatatc ctattgacca ggccagtagt gcgcctaatg ttttcaaaac attcttcaga
      301 gcattaagcc cttacgacta cacactgtat tgcaggaagt gggtaaaacc aaatctgact
      361 tgtacgccac aggatgatcc ccagtatcca gggatggata caaagacagc aagtgattac
      421 ctgccacaga caaattggcc aacaactcaa ttaccaccag gatatacttg tgcagaaccc
      481 tatttattcc cagacattaa taaacccgat gggccagcaa cagcagggat agatgatttg
      541 ggtgaaattt tagctaaaat gaaacagaga acatcgagag gaataagaaa atgaaaagaa
      601 ttttgttctt tttgctgatg atatttgtta gttttggtgt tatagctgat tgcgaaacac
      661 aagctaaaga tcaagattgc tttaccattt tcactaaggg tactattttt tccgcgtttc
      721 ctgtcttaaa taataaaacc atatggagat ggtatcagaa tgaggacata ggtgagtatt
      781 attggcaaac agaacttggt atatgtaaaa ataacaaatt cacaccaagc ggggcgagac
      841 tcttaattcg cgtcggatca ctacgcctta atgaaaatca tgcaaccaaa ggaacgttgc
      901 aggagttgct aaatacagca gaaaaaacgg cgtttctcgg cgatcggttt agaagttata
      961 taagagcggg tatctatcaa aaaaagagca gtgatccagc tcagttgttg gcagtccttg
     1021 ataattcaat catggttaaa tattttaaag atgagaagcc aacttatgct cgaatga
//
LOCUS       DAZGAP010000465         1054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGAP010000465 DAZGAP010000000
VERSION     DAZGAP010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.468"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1054)
                     /locus_tag="ACHLAM_005439"
     CDS             complement(<1..>1054)
                     /locus_tag="ACHLAM_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7856781.1"
                     /translation="AVKPDVLRFRGREALSEPFRWDIGFTSRNANIPPEEVLMKYASL
                     RMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLALLEYTRQCAVFQNQSVPEVVEQ
                     VLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFIQRILSEVGIFWRTEMDDVRGL
                     DTYIFADSQLNYQFDVCLPYSEPSGLFDGAAESVWDVRTWHNVAIGTVSTRDYNYRTA
                     SMPMDATVSVRNDAVTTGEHYRDAPAYREAGDDTSPEPETESGAFYARLHHERELNRS
                     ARIHLFSNAAHLSPGQVLEPQGDVIADLKEGIVLTLVTYRGARDSRLHVSAWGMPYTE
                     RYCFRPAEIPRPVIPGT"
BASE COUNT          227 a          322 c          291 g          214 t
ORIGIN      
        1 gcgtacccgg gatgaccgga cgcgggattt ctgccggacg gaaacagtaa cgttcggtgt
       61 aaggcattcc ccatgctgac acatgcagcc gggaatcccg ggccccccgg taagtcacca
      121 gtgtgagaac aatcccttcc ttcaggtccg caatgacatc cccctgcggc tccagcacct
      181 gcccggggga gaggtgtgcg gcgttgctga acaggtgaat gcgggccgac ctgttgagtt
      241 cccgctcgtg gtgaagccgg gcgtaaaacg caccagattc ggtttccggc tccgggctgg
      301 tgtcatcccc cgcttcacga taagccggtg catcgcggta atgctccccg gtggtgaccg
      361 catcattacg cacgctgacc gtcgcatcca tcggcatgga ggcagtccgg tagttgtaat
      421 cacgcgtgga gaccgtgccg atggcgacgt tgtgccatgt ccgtacatcc cagaccgatt
      481 ctgcagcacc gtcaaaaagg cccgacggct cgctgtacgg cagacacaca tcaaactggt
      541 agttaagctg gctgtcggca aaaatgtacg tatccagtcc gcgcacatca tccatctccg
      601 tgcgccagaa aatccccacc tcagacagaa tgcgctgaat gaactgcaaa tccgtttcct
      661 gccactgggt gataatttca cgcgacgggt aagcgcgttc cagtctgaat tcaaaatcag
      721 cgccctccag cccgtgtttg cgcagcacct gctccaccac ctcagggacg gactggttct
      781 gaaatacggc acactgccgg gtgtactcca gcagtgccag acgggagctg agtgtaagcc
      841 ggtagtggga ctggtctttc gatgtggaca gccattccag acgggtcaca atgccatgga
      901 catttttgcc gttacgcatc cgcaacgacg catatttcat cagcacctct tccggaggaa
      961 tgttcgcatt ccgggaggtg aacccgatgt cccatcggaa cggctcactc agtgcttccc
     1021 tgccacgaaa acgtaaaaca tcaggcttta ctgc
//
LOCUS       DAZGAP010000466         1053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGAP010000466 DAZGAP010000000
VERSION     DAZGAP010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.469"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..344
                     /gene="cdiI"
                     /locus_tag="ACHLAM_005440"
     CDS             <1..344
                     /gene="cdiI"
                     /locus_tag="ACHLAM_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease toxin immunity protein CdiI"
                     /protein_id="HFO7856782.1"
                     /translation="PYERSEPGWTIRSYFDLMYNENRFLDAVENIVNKESYILDGIYC
                     NFPDMNSYDESEHFEGVEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYLQLHPEDTE
                     KVNKLLSKIPS"
BASE COUNT          323 a          230 c          199 g          301 t
ORIGIN      
        1 tgccatatga acgttctgaa cctgggtgga ccataaggtc atactttgac cttatgtata
       61 atgaaaatag gttcttggac gctgtagaaa atatagttaa caaagaatct tatattctgg
      121 atggaattta ttgtaacttt cctgatatga atagttatga tgaaagcgaa cattttgaag
      181 gtgttgaatt tgctgtcggc tatcccccag atgaagatga tatagtgatc gtaagcgaag
      241 aaacctgttt tgaatatgtt cgtctggcct gtgaaaaata tcttcagtta caccctgaag
      301 atactgaaaa agtaaacaaa ttactgtcta aaatcccatc ctgagtaaag attccggcca
      361 ctgcgccggg atctttcttt aaccgtcagg ctcatccaca atgtccatcc ccacgcttaa
      421 tcaccggcac atctctaatg tcgttaagcg caccggggaa ggcgttaaac cacgtgagca
      481 tctgcggcct gttgacatac gttgtgtcgt tacggggaag tcatcttcac agcgattttc
      541 agtcagttag cattaccgac attcattcat caaattaccg gagataattg atgtcaaaac
      601 cgtaagcgta aactgaccgc cgtatgtagc catcagacga gaattggtaa cttagacgcc
      661 catctgatat agacggacat ctaagtatgg aattacagga ctggcgaaaa gaacctcgta
      721 aaaactattc gaatgaattc aaacttcgta tggtggaact ggcatcacaa cctggaggtt
      781 tatcccaaga atattgcctt tgatatgctg gcacatacaa tccttgacca ttctcacaga
      841 tatactccca cacacttttg ggttgcgccc cgaaaacatc tttgatttcc atataacccc
      901 ttgatttaat accatttatc aaaaatttaa catttcggaa ttttaccaac tatggaagac
      961 ttagcaataa ccatcaaaca atactaacta atcatccctt agcaagggct aactctacga
     1021 aacgctatgc tgcttcttcg agttctccat tgt
//
LOCUS       DAZGAP010000467         1015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGAP010000467 DAZGAP010000000
VERSION     DAZGAP010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.470"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(108..803)
                     /gene="aqpZ"
                     /locus_tag="ACHLAM_005441"
     CDS             complement(108..803)
                     /gene="aqpZ"
                     /locus_tag="ACHLAM_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO7856783.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT          318 a          273 c          232 g          192 t
ORIGIN      
        1 tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaacaccc gtgcggtttg
       61 taacgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta attacgcttt
      121 tccagcaggg tccggtaaat cagaccaccg ataataccgc cgacaattgg caccacccag
      181 aagaaccaca gttgttctaa cgcccagccg ccctggaaga tagcagccgc ggtgctgcgc
      241 gccgggttaa cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc
      301 agaccaatag cgatcggcgc aaaaccagca ggagcgaatt tgtcggttgc gccgtggatc
      361 accagcagga aacctgcact caataccagt tcaactacca gcgcggaaag catggaataa
      421 ccgcctggcg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa
      481 cctgttttac cactggcaat taaatacagc agcgccgccg caacaatacc gccgacaacc
      541 tgggcaatta cgtagccaac gacttctttt gccgggaaac gtccgccagc ccataaacca
      601 atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcaaa ggccatcgtc
      661 agaacggtca gaccgaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag
      721 cctgcggcca gtacggcact accacagcca ccaaaaacaa gccagaaagt accaaaacat
      781 tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccctg tgaaaaatat
      841 ggtcgtttta tagggtcgtc gtaaaaaagt gacgacggaa ataatgcgcg gctattttaa
      901 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt aaggcaacag
      961 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcga aagat
//
LOCUS       DAZGAP010000468         1011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGAP010000468 DAZGAP010000000
VERSION     DAZGAP010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.471"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..862)
                     /locus_tag="ACHLAM_005442"
     CDS             complement(<1..862)
                     /locus_tag="ACHLAM_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO7856784.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESLLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGM"
BASE COUNT          238 a          312 c          257 g          204 t
ORIGIN      
        1 ccattcctcc gtcaccaagc gccataccga ccgcagatga caccctcagc cagtcctcat
       61 catccatgtt gccactgcgc aaatgattca gttccacacg ccccagcatg gaaagcatgc
      121 gcagaatcag ttgctccttc ggcatctcaa tgctgaatac cagaacgctc tcttccgttc
      181 gccgcagtgc ggcgctgaga cacatggata gcgccagaga ggttttcccc attgacggac
      241 gggcagccag cagaaccagg tcaccaggct gcaggccaca ggtcatttca tccagacagt
      301 caaatcccgt gggggtaccg gtaacaccac cggagccgag caaactttcc agttgctgca
      361 gaacggtatc aagcgcggat gaaagagagg cttctgtgcg ttcccggagc gtcctgttct
      421 gcgcaagtgc aaacagacgc cgctctgcag catccagcac atcccgtgat gtggcgttcg
      481 gtgccgccac atcagcaatc agggtgtttc cggtggcctg taactgacgc agaacgcttt
      541 tctccaccac aatgcctgca taggccatga tgttggccgc agaaggtgtg ttcttgctga
      601 gctcggccag ataagcaaaa ccgcccagta cgtccagcct gccctgatgt tccaggtgct
      661 ctgacagtgt aatcaggtcc agtggctggc cattcaccgc cagttcagac atggcacgga
      721 aaatggcccg gtgctgagcc acataaaaat cgtcaggact cagcatcagc accacttcat
      781 cccagcgttc attctccagc atcagcccac ccagaacggc ctgctctgct tcgtatgcgc
      841 agagaacaga catttcagcc atggcgtacc ctccctctct gactgccgga aacctggcta
      901 cgtgaaaaaa ggaaaaaggg tgccggacat acagttgtcg ggaataaaat cccggtaact
      961 gtctgtacgt tttcagaccg gtgagtttgc cgaccccggt ctgaaggtac g
//
LOCUS       DAZGAP010000469         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGAP010000469 DAZGAP010000000
VERSION     DAZGAP010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.472"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..355
                     /locus_tag="ACHLAM_005443"
     CDS             <1..355
                     /locus_tag="ACHLAM_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7856785.1"
                     /translation="AAIVGSFFTGGATLAAWGAALSAGGLTATTMLFSLGASMILGGV
                     AQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDIST
                     RDEGGDGKVVVIGRG"
BASE COUNT          292 a          198 c          288 g          229 t
ORIGIN      
        1 tgcagcgatt gtcgggtcat ttttcactgg tggtgcaaca ctggcggcat ggggcgcagc
       61 cctgagtgcc ggagggctga ctgccaccac gatgctgttc tcactgggtg ccagcatgat
      121 actgggtggt gtggcccaga tgctggcccc gaaggcaaag acgccggagt acaaaagtac
      181 ggataacggt aaacagaaca cgtatttttc gtcactggac aacatgattg cccagggtaa
      241 cccgatgccg gtgccttacg gtgaaatgct ggttggttca cgacggatat cccaggacat
      301 cagcacccgt gatgagggcg gagacgggaa agtggtggtt atcgggcggg gataaaaata
      361 aaaaaaatcc cgcagagtta gcggagctgc gggaaataac gatgaagatt aacgttatgg
      421 agttattttt ttgggcagaa aaacattaac gcagcgaaat tataagcgct acatggattt
      481 tgcgaaaatg tgaagaaatt cagaaatttt ttgctgcaga ggtgcaggga gcttgccggg
      541 tatttgtggc agaagacaga aaacatcatg ccggggttaa gggataaaaa aatcccgcag
      601 agtccggaga ctgcgggaca gaaccgatga agattaacgt tcaaggagtt ttgtttttat
      661 tgacccgaaa aaactgtaac gccccggaat gatatctgcc acggggacgt acagaaaatg
      721 tgaagaaatt cagacttttt ctgtcgcagg tttacagaac ggatgaccgc cagggtacca
      781 gtggcgtaaa tgagcaggat gttgtggtgt ggctgaagca aaaaaatctc ccgtaatatc
      841 gccggatact gacgggagac gaatagtcac cttaaggagg tctgataatt attgctttta
      901 ttgagcaaaa aaactgtaac gctgcgtcat tatagtggct acaggcaatt gccggaaatg
      961 tgaagaattt cagaaatttt attccgtcat gacacaggca ccctccg
//
LOCUS       DAZGAP010000470         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGAP010000470 DAZGAP010000000
VERSION     DAZGAP010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.473"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1002
                     /locus_tag="ACHLAM_005444"
     rRNA            <1..>1002
                     /locus_tag="ACHLAM_005444"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          235 a          226 c          319 g          222 t
ORIGIN      
        1 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
       61 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
      121 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
      181 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
      241 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
      301 ttctaacgtt ggcccctgat cggggttgcg gacagtgtct ggtgggtagt ttgactgggg
      361 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
      421 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
      481 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
      541 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
      601 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
      661 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
      721 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
      781 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
      841 agagataagt gctgaaagca tctaagcacg aaacttgccc cgagatgagt tctccctgac
      901 tccttgagag tcctgaagga acgttgaaga cgacgacgtt gataggccgg gtgtgtaagc
      961 gcagcgatgc gttgagctaa ccggtactaa tgaaccgtga gg
//
LOCUS       DAZGAP010000471          997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGAP010000471 DAZGAP010000000
VERSION     DAZGAP010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.474"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..204
                     /locus_tag="ACHLAM_005445"
     CDS             <1..204
                     /locus_tag="ACHLAM_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7856786.1"
                     /translation="TELFRYLSDMGKDINTVLNDDRDFRTLMRETDDPDILELLSTER
                     AIKRLATGINKELDIAFNHLEII"
     gene            214..843
                     /locus_tag="ACHLAM_005446"
     CDS             214..843
                     /locus_tag="ACHLAM_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856787.1"
                     /translation="MSGNATNEIILAKINDILVDFNELNRQINNFPEKFSGKIDAINF
                     AIKQTPDALEESLKKLFSAAEELEKTVQESTDECRVSMRAYAINELDNLKVKISEAIN
                     ESIKNSIDMPLDQAKERIIQINKMLQKSTGVEMSTRVAFGIGGLLGLSFLSLAMCISA
                     GYLYFQERSKAERYYNAFREQQIVIERLPEETQRQFRDELGRYLARNQK"
BASE COUNT          352 a          132 c          209 g          304 t
ORIGIN      
        1 acggagttat ttagatatct ttctgatatg gggaaagata taaacactgt tctaaatgat
       61 gacagggatt ttagaacatt aatgagggaa actgatgatc cagatatact tgagttattg
      121 tcaacggaaa gagctataaa gcgtcttgct acagggataa ataaagagct ggatattgca
      181 tttaatcatc tcgaaattat ataaggtgta aatatgtctg gaaatgcaac aaatgaaatt
      241 attttggcaa aaataaacga tattcttgtc gactttaatg agttaaacag gcaaataaat
      301 aactttcctg aaaagttttc agggaaaata gatgcaatta attttgccat aaaacagaca
      361 cctgatgcgc ttgaggaatc attaaaaaaa ttgttttctg cggcagaaga gttagaaaaa
      421 acagtgcagg aaagtacaga tgaatgtcgg gtatcaatga gagcgtatgc tataaatgag
      481 ttagataatc tgaaagtaaa gatatcagaa gctataaatg aatcaattaa aaattctatt
      541 gatatgccgt tagatcaggc gaaagaacgg attatccaga taaataagat gctacaaaaa
      601 agtaccgggg tagaaatgag tacaagagta gcatttggta ttggtggatt gctggggctt
      661 tcatttcttt ctttagcaat gtgtatatca gcaggatatt tgtattttca ggagcggagc
      721 aaagcagagc gttattataa tgccttccgt gaacaacaga tagtgataga acgtttgcca
      781 gaagaaacgc agcgccagtt ccgggatgaa ttggggcgtt atcttgcccg taatcagaag
      841 taaagtaggc cctagttagg tgtaccttag gttaattata ccttagttat accttagtta
      901 tatgttaatt atatcttagt tagctacacc ttgagttagt tatacgttaa ttatacctta
      961 gttatacgtt aattatatct tagttatacc ttaatta
//
LOCUS       DAZGAP010000472          990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGAP010000472 DAZGAP010000000
VERSION     DAZGAP010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.475"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..402
                     /locus_tag="ACHLAM_005447"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHLAM_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            397..777
                     /locus_tag="ACHLAM_005448"
     CDS             397..777
                     /locus_tag="ACHLAM_005448"
                     /inference="COORDINATES: protein motif:HMM:NF026977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856788.1"
                     /translation="MNKTVEFSRGPQTYVSDLGHVTGSTARARNKAITAILKEDFAYL
                     KLTYIPQYNPFMRTGIAKLWEGTQFGKNSFSSRAALRDVIIHEELHHRWWKRGLNDHH
                     PKGTEMEQKFYETIRRYKRMRGWL"
     gene            777..>990
                     /locus_tag="ACHLAM_005449"
     CDS             777..>990
                     /locus_tag="ACHLAM_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856789.1"
                     /translation="MFGDEFKTTIECPCCGKICSIAIGQTSHNGILAWYESIICHNCG
                     LRSESDDIGFPPEKIRLQLIDFEGLWN"
BASE COUNT          270 a          195 c          271 g          254 t
ORIGIN      
        1 ccgtccgtga ccccgcagtg ggtgacgcgg acgaattatg cggtgagcga cctgacaggc
       61 cgcccgctga tgctctttaa cagtgaaggt aaaaccgtct ggcggccggg acagaccagc
      121 ctgtggggac tggcactcag cctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataatcggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gatccgctgg ggttgctggg gggtgagcag acttaccggt atgtgcctaa tccgtgtggg
      361 tatgttgatc cgctgggatt agctataact agtataatta ataaaacagt cgagttttct
      421 agagggccac agacttatgt ttctgatctc ggtcatgtta caggttctac ggccagagcc
      481 agaaataagg caattacagc catacttaaa gaagattttg catatcttaa actaacatat
      541 atccctcaat acaatccgtt tatgcgaaca gggattgcta aactttggga aggaactcag
      601 tttggtaaaa atagtttttc gtctcgtgca gcattaagag atgttattat tcatgaagaa
      661 ttacatcata ggtggtggaa gcgaggattg aatgatcatc atcccaaagg tactgagatg
      721 gaacagaaat tttacgagac gattcgaaga tataagcgga tgagagggtg gttatagtgt
      781 ttggtgatga atttaaaacg actattgagt gtccttgctg tggaaaaata tgttctatag
      841 ctatagggca gacgagtcac aatggtatac tcgcatggta tgaatcaatt atatgccata
      901 attgcggatt acgtagtgaa tcagatgata taggctttcc cccagaaaaa attcgtctac
      961 aattgattga ttttgagggt ttatggaatg
//
LOCUS       DAZGAP010000473          990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGAP010000473 DAZGAP010000000
VERSION     DAZGAP010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.476"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..586)
                     /locus_tag="ACHLAM_005450"
     CDS             complement(<1..586)
                     /locus_tag="ACHLAM_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO7856790.1"
                     /translation="MNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTT
                     YRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAASQLVTQRAGF
                     FPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASG
                     TGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD"
     gene            complement(617..778)
                     /locus_tag="ACHLAM_005451"
                     /pseudo
     CDS             complement(617..778)
                     /locus_tag="ACHLAM_005451"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          178 a          301 c          264 g          247 t
ORIGIN      
        1 ggtcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccacaac
       61 tgtcatagcc ataggactca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctgaccgttc cggtccagcc ggtattcccg ccgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacagcc gacacattgc ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgaggggag cagcccgccc catgtggttt cctccgggaa aaatccggcg cgctgggtga
      301 caagctggct ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcaccgg
      481 gatgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtagtggta ttcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgac ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca gaccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggctactc cgttgtaccc agctcataga atttatctag
      841 caatttgtat taagggggtt tcacccacat gttttaataa actcattaac cagatggcct
      901 cttctctcgt tccggagtat attaccttcg attcatttct aataattcga agtgcttctt
      961 ttgtatcaat cgaaaaagcc ttacgaatga
//
LOCUS       DAZGAP010000474          980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGAP010000474 DAZGAP010000000
VERSION     DAZGAP010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.477"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..695
                     /gene="ydeO"
                     /locus_tag="ACHLAM_005452"
     CDS             <1..695
                     /gene="ydeO"
                     /locus_tag="ACHLAM_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO7856791.1"
                     /translation="AFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNI
                     QTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFATCKGFI
                     TLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILL
                     DARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTG
                     MNTDNTMSALAI"
BASE COUNT          310 a          178 c          191 g          301 t
ORIGIN      
        1 acgccttctc tgacggcgag atcctgatgg tagataacgc tgttcgtacg cattttgaac
       61 cttatgagcg gcatttcaaa gaaatcggat ttaatgaaaa taccattaaa aaatatctac
      121 aatgcactaa catccagaca gtgacggtgc ctgttcctgc gaagttttta cgtgcttcaa
      181 atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa gaacgcaatc
      241 atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc acatgcaaag
      301 gtttcattac actattaact aacggtgtgc tatccgtttc cgggaaagtg agaaatattg
      361 tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc ctgtacatta
      421 gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca cagattcttt
      481 tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca gtcaataaaa
      541 ttgccgaaca atgtggttat gcgagtacat cttattttat ttatgcgttc cgcaaacatt
      601 tcggcaacag tccgaagaga gtttctaagg agtaccgttg tcaaaggcac acgggtatga
      661 acacggataa cacgatgagt gctttagcta tttgattatt tgctaacgag tagtcaacca
      721 cgcacgttac gtagaaatta aaatggggca gccattccct gccccgcgtt gtttttaggc
      781 gatatattat tgaaataaat aagtgacatt catcacatat ttatgcattt gcataacctg
      841 ttgcatgatt atttatgatc tcaattcgga attttgtcag taaaatacaa taatttatta
      901 aatatcaata gattagttgt ttatctgcga gaaattactt agtagaatag aaagtaatgt
      961 caacagttta tggactgtta
//
LOCUS       DAZGAP010000475          980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGAP010000475 DAZGAP010000000
VERSION     DAZGAP010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.478"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHLAM_005453"
     CDS             <1..573
                     /locus_tag="ACHLAM_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7856792.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGTSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQ
                     AALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTHAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            628..>980
                     /locus_tag="ACHLAM_005454"
     CDS             628..>980
                     /locus_tag="ACHLAM_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7856793.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIV"
BASE COUNT          226 a          267 c          269 g          218 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggtac ctccagctac cagatgttac ccggatattt tcgtgccatt
      121 tgtaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtcat agaaggcgct tacgaagtgg tgggcgtgtt tgaccggatt
      241 gaggaaaagc gtgatgccat gcagtcgctg gtcctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt gacgaacatc agcccgtcac caccgccgac
      361 attctgacgc cacgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 attcaggaga atatgctgaa aggtggaatt tcaggtcgca gcgctaaagg aaaacgtatc
      481 catacccatg ccattcacag cattgacact gacattaagc tcaaccgcgc attgtgggtg
      541 atggcggaaa cgctgctgga gagcctgcgc tgattcagcc attaccctga aaaccacgca
      601 tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc caccacatct
      661 tctgtttccg taaccgctgc atcagggaat gaccagtctc agctggttgc cactcccgtc
      721 cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc acagtgggtg
      781 accctggagc cccgtatctt cggctggatg gaccgtcttt gcgaagacta ctgcggtggt
      841 atctggaatc tgtacacact gaacaacggt ggcgcattta tggcccccga accagatgac
      901 gatgatggcg aaaactgggt actgttcaat gccatgaacg gtaaccgcgc tgaaatgagc
      961 ccggaagccg ccggcattgt
//
LOCUS       DAZGAP010000476          980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGAP010000476 DAZGAP010000000
VERSION     DAZGAP010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.479"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..280)
                     /locus_tag="ACHLAM_005455"
     CDS             complement(<1..280)
                     /locus_tag="ACHLAM_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7856794.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMP"
     gene            complement(361..903)
                     /locus_tag="ACHLAM_005456"
     CDS             complement(361..903)
                     /locus_tag="ACHLAM_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7856795.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDISAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          284 a          214 c          182 g          300 t
ORIGIN      
        1 tcggcataat tttaattttt acttgctgcc cggaatttgc ccctattttt gccactggtg
       61 gcgttaacat gaaaggaacc tgaatttttt ctggtggtaa tgacgtatcg ccatcatcaa
      121 tccacgcctg cagcagcgaa gaacggtttc cctggttcat caactgcacc atcacttctt
      181 tattttctgc cgggtaaata atgcgcgtcc cgtagataac aataccggca tgtgcaggaa
      241 ggaccataga agaaatgagt aaagctaata atcctttcat cttaatgcct cttaaattaa
      301 aaggccgcca tggggatagc ggccatgttt attacctgga gaaacttgca attttattta
      361 ttaatcctgc gccacacgca taaccagaga agataacaca tcacctgatt gcactgaatt
      421 atctgtcagc ttcaggtatt ttgctttaaa cggaatagca tattgacctt ccgctgtttt
      481 ataactctca atgttaaaag gtgcagaaat atccaggaca tcactgttat cacctaaata
      541 gagatagtaa cccacggtat cgttgctact ggctatcgcc ttatcgttgc cggttatttt
      601 gtcgtttgtg atcaacgaga gttttacacc agcaacttct ttcgggcagc taatttttaa
      661 catgctgctg gttacaccga ctgcatcatc ataggtagaa tatttagctt taccttcaaa
      721 gacagtggtt ggtacagttg gcatattaat acttactgaa tcgccacctg cttcgaattt
      781 acacggagat tctgcaatat tccctttaat ggttaatgtt ccggtatcaa cgtctgcggc
      841 aaatacccca gcgctcataa aaaaagaaga aaaaacagcg gcagcaataa ttgaacgttt
      901 cataatatgc tccttaataa aatctatttt tggttgttaa gagtctgtat ctcttggaaa
      961 caaatttctc acacccaaaa
//
LOCUS       DAZGAP010000477          961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGAP010000477 DAZGAP010000000
VERSION     DAZGAP010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.480"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>961
                     /locus_tag="ACHLAM_005457"
     CDS             <1..>961
                     /locus_tag="ACHLAM_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7856796.1"
                     /translation="NVTFIGRPVGADTAGIPSGDLPPVLQRALDKFTRDITASAREGK
                     IDPVSGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRP
                     VTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGG
                     ADAANLLKPALARGELRTIAATTWSEYKQYLERDAALERRFQMVRVDEPDDETACLML
                     RSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTV
                     PEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEEN"
BASE COUNT          201 a          269 c          296 g          195 t
ORIGIN      
        1 aatgtgacct ttatcgggcg tcccgtcggt gcggatacgg cgggtatacc gtcaggtgac
       61 ctgccgcctg tgttacagcg tgctctggat aaattcaccc gggacatcac ggccagcgcg
      121 agagagggga aaattgaccc ggtatcggga cgcgatacgg aaatccgtca gatggtggat
      181 attctctccc gccgccgcaa gaacaacccg attctggtgg gggaccccgg tgtggggaaa
      241 acggctctgg tggaagggct cgccctgcgt attgcggaag gtaacgtacc ggaatcactc
      301 agacccgtca ccctgcgcac ccttgacctc ggcctgctgc aggccggtgc cggcgtgaag
      361 ggtgaatttg aacagcgcct gaaaaacgtg attgatgccg tgcagcagtc accggctcct
      421 gtactgttgt ttatagatga agcccatacc cttatcgggg ccggtaatca ggccggcggc
      481 gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg gtgaactgcg taccattgcc
      541 gccaccacct ggtctgagta caaacaatac ctggaacgtg acgcggctct ggagcggcgt
      601 tttcagatgg tcagagtgga cgagccggat gatgagacgg cctgtctgat gctgcgctcc
      661 ctgaaatccc gttatgcgga acatcataac gtgcatatca ccgatgaggc ggtacgtgct
      721 gccgtcacac tgtcgcgccg ttatctgaca gaacgtcagt taccggacaa ggccgttgac
      781 ctgctcgata ctgctgctgc ccgtgtgcgt atgagcctcg acacggtgcc ggaacagctg
      841 acccgtatcc gttcacagct cgcttccctc ggtatggaaa aacaggcact gctggaagat
      901 attgccgttg gtcatcagaa tcacggcgaa cgtctgtctg ccattgagca ggaagagaat
      961 g
//
LOCUS       DAZGAP010000478          958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGAP010000478 DAZGAP010000000
VERSION     DAZGAP010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.481"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..480
                     /locus_tag="ACHLAM_005458"
     CDS             <1..480
                     /locus_tag="ACHLAM_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7856797.1"
                     /translation="RIIKAGKKLKKDNPNYNGDLGFKIFETVHDFRAKDESELTLSNL
                     SFFDDAVLTPEQYDTLLTTWCVYDGSLLTTPIEDIDLNGYKAHLCDGRLYLIAPNFTS
                     EALKALLQKLDADKDFAPNKVVFYGSNFESAKQMELNEALKSYANKKSIDLDLVVRN"
     gene            483..>958
                     /locus_tag="ACHLAM_005459"
     CDS             483..>958
                     /locus_tag="ACHLAM_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO7856798.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYERELKTYVVESQKSSGKNT"
BASE COUNT          339 a          165 c          180 g          274 t
ORIGIN      
        1 agaattatta aggcaggaaa aaagctgaaa aaagataatc ctaattataa tggagatttg
       61 ggatttaaaa tatttgaaac agttcatgat tttcgagcga aagatgagtc tgaacttacg
      121 ctttctaatc ttagtttctt tgacgatgct gtattgacac cagagcagta tgacactcta
      181 ttgacgacat ggtgcgtgta tgatggcagc ttgctaacaa caccaattga agatattgac
      241 cttaatggct acaaagcaca cttgtgcgat ggtcggctat atctaattgc gcctaatttc
      301 acaagtgagg ctttgaaagc tttacttcaa aagctggatg cagacaaaga ctttgcccct
      361 aacaaagttg tattttatgg cagcaacttc gaaagtgcga aacagatgga actcaatgaa
      421 gcactaaaaa gttatgctaa taaaaaatct attgatttag atttggtggt aaggaactaa
      481 tcatgtctaa agggttcaca ttcgaaaaaa atttacctca tcaaaaggct ggcgttgatt
      541 cggtaatgaa tgtctttgta tccgccatac ctcatcagac ggatcatgtt gcaattcgcc
      601 tgttagctaa tccagaactg aatctatctg aacaacaata ctacaacaac ataaaaaatg
      661 ttcaggagtt caatggtatt gagcactcaa aagataatta cgatgctaaa agcaacgtaa
      721 ttgatgtttc tatggagaca ggtacaggta aaacttatac ttacaccaaa acaatttttg
      781 acctgaacaa atcatttggt attaacaaat ttatcatcat tgttcctacc ctatcgatca
      841 aagccggaac cgttaacttt ttaaaaagcg atgctataaa agaacacttt cgagatgatt
      901 acgagcgtga actaaagact tacgttgttg aaagtcagaa aagttcgggt aaaaatac
//
LOCUS       DAZGAP010000479          943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGAP010000479 DAZGAP010000000
VERSION     DAZGAP010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.482"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..682
                     /locus_tag="ACHLAM_005460"
     CDS             <1..682
                     /locus_tag="ACHLAM_005460"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO7856799.1"
                     /translation="LPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAV
                     GTLDVPMIDFSSVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVVKNNKH
                     SGLDFSLHRFSKLIAEATPADINISGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIP
                     GTFLTGGTVGTPWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKM
                     VSGWSHPA"
     gene            645..>943
                     /locus_tag="ACHLAM_005461"
     CDS             645..>943
                     /locus_tag="ACHLAM_005461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856800.1"
                     /translation="MKKWSLAGVTLHNYGVNGYRNNWLLLPEDYIRNIIVADFDPIIS
                     FNKNSKEHMSWTYDAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTGENGIID"
BASE COUNT          293 a          176 c          214 g          260 t
ORIGIN      
        1 tttgccgggt aaagcatcaa cggtcagcgc agaaatacca tatcagactt ttcgtgactt
       61 tgctgaaaat aaaggtgtgt ttacccccgg agtgacagga attgaaataa aagataataa
      121 tggaaatgca gttggtacgc ttgatgtgcc aatgattgac ttcagcagcg tgtcacggcg
      181 gggctcactg accctgttgt cgcagggata tggtgtatca gcaaaacacg gagaccttgg
      241 tgatgtaaac aatgccagct ttggctatga taaaaataat tatactgtag taaagaacaa
      301 caaacattcg ggccttgatt tttctctgca tcggttcagc aaactcatcg cagaggctac
      361 acctgctgat ataaatatat ctggacaact tagtgattcg tcacagtata ctgcttttta
      421 cagagcaggc gctggcacac agtatattaa agaacgctca ggtaaacaga ctcatatccc
      481 gggaacattt ttaactggtg gaacagtagg cactccatgg tattcaggaa ataatttaat
      541 atcaagctct ccaggtgaca cctataataa atcacaggga ccattagcca gttatgggca
      601 gatgggtgac agcgggtcgc cattatttgc ttatgattct ctcagtgaaa aaatggtctc
      661 tggctggagt caccctgcat aactatgggg tgaacggtta cagaaataac tggttgttat
      721 tacctgagga ttatatcaga aatattattg tagccgattt cgatcccatt atttcattta
      781 ataaaaacag taaagaacat atgtcatgga catatgatgc agccaaaggc gtgggccgta
      841 ttcagcagga tgaccaacag tttgtgatgc atggtaacct gaatggtaat ctgaatgcag
      901 gtaaaaatct ttattttaca ggcgaaaatg gcattattga ttt
//
LOCUS       DAZGAP010000480          938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGAP010000480 DAZGAP010000000
VERSION     DAZGAP010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.483"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..248
                     /locus_tag="ACHLAM_005462"
     CDS             <1..248
                     /locus_tag="ACHLAM_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856801.1"
                     /translation="GLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKE
                     GRVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(266..613)
                     /locus_tag="ACHLAM_005463"
     CDS             complement(266..613)
                     /locus_tag="ACHLAM_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO7856802.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            732..932
                     /locus_tag="ACHLAM_005464"
     CDS             732..932
                     /locus_tag="ACHLAM_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856803.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
BASE COUNT          258 a          234 c          225 g          221 t
ORIGIN      
        1 caggtctgcc cgcagacggc acttctgcca ctgatctggc ggtggagctg aacggcataa
       61 cgtatcaggc ctgccgtggg gattttgtgg tgcgtctcga cggcagcacc tgtctgcagt
      121 tatggaataa agaaggcaga gtggtacgcc gggagggcga tccgctggaa gtggcgcagt
      181 ggctgcaggc ctgtcacgat gcaggaatgg aggtccgtgt gcaggttaat gaaagcatca
      241 ccccgtaaaa acatgcctgc acggtttaca catcatccca tggtggcagc cggttaactt
      301 tcctgcgcca ccagtggaaa gaggccgtga gcagtccgaa caaaaaacca cccgtgatac
      361 ttataataat ggcctcacct gccaccattc ctgacggccc ccagtagata aaccacattg
      421 cacatcccca ggaaatgccc cataagctcc ccatcaacag cgtgacctgc cagaacggca
      481 taaagggtaa tggcggaagc cggataccca gccgccagag aatacgcaga agaggagggg
      541 cataattgct ccgccacatc tttttgctat ccatcagggc aatggcccgg gcttttttct
      601 gctcaaaggt cacaggagca cctccgttcc atgatgtaaa cgtgacacag cttattgtta
      661 ttgattatta ttatcatgct gtgattatgt tgtcatgcac cggtacacga taacaataaa
      721 ggagaaacca gatgaaactg gatatatctg taaagtattt tcttaatatt atgatagcag
      781 atttcatcat tcccgcacca gaaaaaatat caaccacatg gcactcagat actaaaatga
      841 atcgctttgc ttgccctata cattctacta atagtgatta ctattcaggt ttttatcata
      901 cagatgtatt aattcctgga gaactggctt aggacgcc
//
LOCUS       DAZGAP010000481          917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGAP010000481 DAZGAP010000000
VERSION     DAZGAP010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.484"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..131
                     /gene="ykgR"
                     /locus_tag="ACHLAM_005465"
     CDS             30..131
                     /gene="ykgR"
                     /locus_tag="ACHLAM_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO7856804.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            496..759
                     /gene="ykgM"
                     /locus_tag="ACHLAM_005466"
     CDS             496..759
                     /gene="ykgM"
                     /locus_tag="ACHLAM_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO7856805.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            759..899
                     /gene="ykgO"
                     /locus_tag="ACHLAM_005467"
     CDS             759..899
                     /gene="ykgO"
                     /locus_tag="ACHLAM_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO7856806.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
BASE COUNT          286 a          188 c          189 g          254 t
ORIGIN      
        1 gcactctttt ttaaagcgag gaagtaccaa tgaaagagaa taaagtacag caaatcagtc
       61 ataaactgat taatatcgtt gtttttgtcg caattgtaga atacgcctat ttatttctcc
      121 atttctatta ataacggcaa taaactgttc acttcagtga tatttaaaat atgcatcctc
      181 tccctttttt gtaagtaatt attatatccg tgggagagga atacacattg tcaggtaatc
      241 aatcatgctg caataaatca tcggccagta aagtggagat agcctccatt ctcgaaaaat
      301 ccatactctc agcgaaacca tcatcaatca ctcatccagg cgtttatggg agcgtcgcca
      361 atggctgcta acaatgccag acttccccgt tgcggaaatt ccacatccca caaatagtca
      421 cagtgattgg gtgttgaaat gatccggatg agcatgtatc tttacggtta tgttataaca
      481 taacaggtaa aaatgatgaa acccaatatc catcctgagt atcgtactgt ggtgttccac
      541 gacaccagcg ttgatgagta ctttaaaatc ggctcgacta tcaaaacaga ccgtgagatt
      601 gagctggatg gcgtaacgta tccgtacgta acaattgatg tctcttcgaa atcgcacccg
      661 ttctatacag ggaagctgag aacagtggca tcagaaggaa atgttgcacg gttcacccaa
      721 cgttttggtc gttttgttag cacgaaaaag ggggcatgat gaaagtcctt aactctctgc
      781 gtaccgcaaa agaacgccat ccagactgtc agattgtgaa gcgaaaagga cggctgtatg
      841 tgatttgtaa atctaatcca cgttttaagg ccgttcaggg tcgtaagaaa aaacgttgat
      901 tcaaaattcg acgaatt
//
LOCUS       DAZGAP010000482          915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGAP010000482 DAZGAP010000000
VERSION     DAZGAP010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.485"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>915
                     /locus_tag="ACHLAM_005468"
     CDS             <1..>915
                     /locus_tag="ACHLAM_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7856807.1"
                     /translation="NVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGK
                     IDPVSGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLSP
                     VTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSSAPVLLFIDEAHTLIGAGNQAGG
                     ADAANLLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVRVDEPDDETACLML
                     RSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTV
                     PEQLTRIRSQLASLGMEKQALLEDIAVGH"
BASE COUNT          186 a          256 c          283 g          190 t
ORIGIN      
        1 aatgtgacct ttatcgggcg tcccgtgggt gcggatacgg cgggtatacc gtcaggtgac
       61 ctgccgcctg tgttacagag tgctctggat aaattcaccc gggacatcac ggccagcgcg
      121 agagagggga aaattgaccc ggtatcggga cgcgatacgg aaatccgtca gatggtggat
      181 attctctccc gccgccgcaa gaacaacccg attctggtgg gggaccctgg tgtggggaaa
      241 acggctctgg tggaagggct cgccctgcgt attgcggaag gtaacgtacc ggaatcactc
      301 agccccgtca ccctgcgcac ccttgacctc ggcctgctgc aggccggtgc cggcgtgaag
      361 ggtgaatttg aacagcgcct gaaaaacgtg attgatgccg tgcagcagtc atcggctcct
      421 gtactgttgt ttatagatga agcccatacc cttatcgggg ccggtaatca ggccggcggc
      481 gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg gtgaactgcg taccattgcc
      541 gccaccacct ggtctgagta caaacaatac tttgaacgtg acgcggctct ggagcggcgt
      601 tttcagatgg tcagagtgga cgagccggat gatgagacgg cctgtctgat gctgcgctcc
      661 ctgaaatccc gttatgcgga acatcataac gtgcatatca ccgatgaggc ggtacgtgct
      721 gccgtcacgc tgtcgcgccg ttatctgaca gaacgccagt taccggacaa ggccgttgac
      781 ctgctcgata ctgctgctgc ccgtgtgcgt atgagcctcg acacggtgcc ggaacagctg
      841 acccgtatcc gttcacagct cgcttccctc ggtatggaaa aacaggcact gctggaagat
      901 attgccgttg gtcat
//
LOCUS       DAZGAP010000483          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGAP010000483 DAZGAP010000000
VERSION     DAZGAP010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.486"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..150
                     /gene="rpmE"
                     /locus_tag="ACHLAM_005469"
     CDS             <1..150
                     /gene="rpmE"
                     /locus_tag="ACHLAM_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO7856808.1"
                     /translation="GVTYPYVTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVG
                     TQKGE"
     gene            150..290
                     /gene="ykgO"
                     /locus_tag="ACHLAM_005470"
     CDS             150..290
                     /gene="ykgO"
                     /locus_tag="ACHLAM_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO7856809.1"
                     /translation="MKVLNSLRTAKERPPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
BASE COUNT          219 a          190 c          211 g          286 t
ORIGIN      
        1 ggtgtgacgt atccgtatgt gacgattgac gtttcctcca aatcgcatcc gttctatacc
       61 ggtaaactta gaacggtggc atcagaaggc aatgttgccc gctttactca gcgttttggt
      121 cgtttcgttg ggactcagaa gggggaatga tgaaagtgct taattctctg cgaacggcca
      181 aagagcgacc tcctgactgt cagatagtga agcgcaaagg gcgattgtat gtaatttgta
      241 aatccaatcc tcgttttaag gctgttcagg gacgcaaaaa aaagcgttga ttccgaagct
      301 tgtacataga ctgtgagcag tgctcagaca tgatttatgt cggtacatta gcattgatgt
      361 gtgtcggcat taattaattt ttgtggatgc gttctatcat tcatatcttc ctgtgctttg
      421 ctgcttgatg ggaaatcctc ttccaatatc acggcgatgt tcactttttg cgtaagtgga
      481 catcgctgcg tattttttgt tagttggggt ttgaggctca gtggaacaat aaccgccgtt
      541 aagcggtcat tttaaatagc caaaaatcaa cccaaaaatc aaccagccaa tttacatcgt
      601 gaatctgcgt tcttggctat ctgttttggg ggggccaagt tggctttagt catcctggcc
      661 gtttgcggaa caaaagacgt tgttgatctc aacagattga tttacctgtt attgaccatt
      721 caattgaacc gcttaaccgc cttttgagtt ccactactcc ccaaacccct agtttagttt
      781 ttagtttcca acggtggtaa tttttcaagt gtcagtagtt cctgcccctt tacccgacgg
      841 cattgggaag ctgcatcctg aagtttttcg cgaacgttat tatcatagtc gggatccaat
      901 agttgg
//
LOCUS       DAZGAP010000484          898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGAP010000484 DAZGAP010000000
VERSION     DAZGAP010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.487"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..373
                     /locus_tag="ACHLAM_005471"
     CDS             <1..373
                     /locus_tag="ACHLAM_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7856810.1"
                     /translation="AISCRPLIENGSIEPRYVDAIYHSHDTIGPYYVVGPGIAMPHAR
                     PEEGANKLSLALTLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFDNEK
                     DIQAILTAKTTQDILSVIARY"
     gene            389..676
                     /locus_tag="ACHLAM_005472"
     CDS             389..676
                     /locus_tag="ACHLAM_005472"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7856811.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TTSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            689..>898
                     /locus_tag="ACHLAM_005473"
     CDS             689..>898
                     /locus_tag="ACHLAM_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFO7856812.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQ"
BASE COUNT          230 a          196 c          237 g          235 t
ORIGIN      
        1 ggcgatttca tgccgaccgt tgattgaaaa cggctctatc gaaccgcgtt atgttgatgc
       61 gatctatcac tcgcacgaca ctatcggtcc gtactatgtg gttggccctg gtattgcgat
      121 gcctcatgcg cgcccggaag agggggctaa caagctgtca ttagcactga ccctgatccc
      181 ttcaggtgtg aattttgatg ccgatgaaaa cgatccagtg aaattactga ttgtgctggc
      241 tgcaaccgac agtaccagcc acattgaagc gatttctcaa ctcgccaagt tattcgataa
      301 cgaaaaagac atacaggcca ttttgacggc caaaacgacg caggacattt tgtctgttat
      361 tgcgcgctat taatccagag gaacatttat gaaaattaca gtggtttgcg gtaacggctt
      421 aggcaccagc ctgatgatgg aaatgagcat taaaagcatt ttgaaggatt tgtccgtcag
      481 cgctgatgtt gatcatgtcg acctgggctc cgcgaaaggg acgacgagtg atatctatgt
      541 cggcaccaaa gacattgccg agcagttagc cgcgcagtct gtcgcaggca aaatcgtcgc
      601 actggaaaat atgattgata agaaagccat gagagaacgt ttgtccgtgg tattaaccga
      661 attaggcgca ctgtaagctg ggggcgatat ggatttcttt cgttttttga tgagcgatgt
      721 actttccgaa cccgcagtgc tggtcggttt aatcgccctg attggcctga ttgcacagaa
      781 aaaaccggta accgaatgca ttaaaggtac cgtcaagacc attatggggt ttgtgatttt
      841 aggcgcaggg gcgggtttag ttgtgtcgtc actgggtgac ttcgcaaaca ttttccag
//
LOCUS       DAZGAP010000485          898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGAP010000485 DAZGAP010000000
VERSION     DAZGAP010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.488"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..89
                     /locus_tag="ACHLAM_005474"
     CDS             <1..89
                     /locus_tag="ACHLAM_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7856813.1"
                     /translation="WRRQIDKRAAEKISASQSAGSKPEEPLI"
     gene            130..375
                     /locus_tag="ACHLAM_005475"
     CDS             130..375
                     /locus_tag="ACHLAM_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7856814.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGDPTPDSDMMM"
     gene            452..667
                     /locus_tag="ACHLAM_005476"
     CDS             452..667
                     /locus_tag="ACHLAM_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7856815.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            671..>898
                     /locus_tag="ACHLAM_005477"
     CDS             671..>898
                     /locus_tag="ACHLAM_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO7856816.1"
                     /translation="MNKKYELVVKGINHYPDKITVTVALEIAGQPSLLSPYVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDIAAALCEGD"
BASE COUNT          230 a          196 c          274 g          198 t
ORIGIN      
        1 tctggcgcag gcagattgat aaaagggctg ctgagaaaat cagcgccagt cagtcagccg
       61 gaagcaaacc cgaagagccg ctcatttagc ggcaactttc ttaacaaccc ttttcaacga
      121 gaaaatccca tgtcagaaat cacatccctg gtcactgcag aggcagtgaa ggaagtcctg
      181 cgctctgaag aagtcctgag cgcactgaaa cagaaactcc gccagaacct tgaggcgcgt
      241 cttgatgcag aagtggatgc cattctggat gaactgctgg gcgcaccggc agttccggag
      301 ccggaaggta tcgcgggtga ggggagtgct tcagatggcg gtgaccccac acctgacagc
      361 gacatgatga tgtaagcatg cgcaagggac catcggtgtg tgccggtggt ctttatattg
      421 ttgtgagctt ccggattgcg ggagacgggg tatgtaccag atggaaaaaa tcacaacagg
      481 tgtgtcatac accacgtcag cggtgggaac gggctactgg ttcctgcagt tgctggacag
      541 ggtttccccg tctcagtggg cggcaatagg cgtgctgggg agtctgctgt ttgggctgct
      601 gacatatctg acgaacctgt atttcaaaat cagagaggac cgtcgtaagg cggcgcgggg
      661 agagtagacg atgaataaaa aatatgaact ggttgtaaaa gggataaatc attacccgga
      721 taagataact gttactgtgg cactggaaat tgccgggcag ccgtcgttgt tgtcgccata
      781 tgtggcgatt agtcttgacc gtactgaagg tgccacgctg gaattttacg aagctgaggc
      841 gaaaaaacag gcgaagcagt ttttcatgga tattgctgcc gctttatgtg aaggggat
//
LOCUS       DAZGAP010000486          894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGAP010000486 DAZGAP010000000
VERSION     DAZGAP010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.489"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(161..832)
                     /gene="queE"
                     /locus_tag="ACHLAM_005478"
     CDS             complement(161..832)
                     /gene="queE"
                     /locus_tag="ACHLAM_005478"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO7856817.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          231 a          225 c          201 g          237 t
ORIGIN      
        1 ttttcactcc agcgaaaaat taacgccaca gaatttgtag agaatgataa tgattagtcc
       61 gggatgggca agatggatga gttttcagag gcgacaaaca atataaatga gtagaagtct
      121 ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata tttagatatt
      181 tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg cacaaacgtg
      241 tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta tcatcggtca
      301 gcgttgccag tagttcatcc agtgcttcaa tatcacgtac gcgccccact ggatgcttga
      361 tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc atgttcagct
      421 ttggtgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta ccactggttt
      481 caatctggca gctaaaacca ttcttttcga gcagatcagt cagtggtagc aaatcatgaa
      541 tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg cgaccaatga
      601 cagccagcaa gtcttcactg ctcgccgccc cccacttatc actctctttg gtcttcgcca
      661 gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc
      721 accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg acgccggtaa
      781 agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc atagcattct
      841 ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat gtct
//
LOCUS       DAZGAP010000487          886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGAP010000487 DAZGAP010000000
VERSION     DAZGAP010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.490"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..697)
                     /gene="reiD"
                     /locus_tag="ACHLAM_005479"
     CDS             complement(<1..697)
                     /gene="reiD"
                     /locus_tag="ACHLAM_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFO7856818.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRHKNL
                     LDSIKAYIDHNFYEPIRLDALATKFNVSPYYVSHEFKRRYGYSPMDYLIKRRLGEAQS
                     LLTTDEGGCEKITS"
BASE COUNT          268 a          203 c          157 g          258 t
ORIGIN      
        1 ttgaggtaat tttttcacac ccaccttcat cagtggtaag taaagattgt gcctccccta
       61 accgacgttt aatcaaatag tccattggcg aatatccata gcgccgttta aattcatgag
      121 aaacatagta agggctgaca ttaaacttag tcgccagagc atccagacgg atgggttcat
      181 agaaattatg gtcaatataa gcttttattg aatccagtaa atttttatgg cgccgtgtta
      241 tatcattacc tgtgcgcatt aatacatttc cctcgacgta tttaagcagc accctggtca
      301 gcaactgcgc cattgttggt ccttctactg tatgctgatt tataacatta aatattgaac
      361 tcatcaattt ctcgatcgtc gcataatgat gatatagtgg aattacgggc acagtatcat
      421 ctggaatcag agcattctcc cgcagacccg ggcacacaat attgtttatc ccacagcaaa
      481 tagtacccac aggccgtccg ctttcaagat actcatcatg cactacttcg ctgttataaa
      541 taaccaaatc accttttctg actgggtaaa tgcgatcacc gatgaaatac tccccctttc
      601 cctcggtgat aaggattagc tccacggtcc tgggatgcat atgcatgaac cgtacaaacg
      661 ggccattgct ttcgctaacc tgactgacat acgccaactg agcccgggac ttattatctt
      721 gttctgaagg acagaagcaa aaacgcatga tttttcctcc attatccgaa tctgtagtgc
      781 tacggacaaa actaaaacta tttttttata atataaaaaa acagaataaa acgttacata
      841 gatcatatta ttttctggtg aaactcatct aacacggata agatca
//
LOCUS       DAZGAP010000488          880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGAP010000488 DAZGAP010000000
VERSION     DAZGAP010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.491"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>880
                     /locus_tag="ACHLAM_005480"
     CDS             <1..>880
                     /locus_tag="ACHLAM_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7856819.1"
                     /translation="EYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYD
                     AAGRVISLTNENGSHSDFSYDALDRQVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVIL
                     WYYDESDRITHRTVNGEPAEQWQYDGHGWLREISHLSEGHRVAVHYGYDDKGRLTGER
                     QTVENPETGELLWQHETKQAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPL
                     LEYMRDRLHRETVRSFGSMAGNTAAYELTSTYTPAGQLQSRHLNSLVFDCDYNWNDNG
                     DLMRISGPRQTREYGY"
BASE COUNT          213 a          247 c          291 g          129 t
ORIGIN      
        1 atgaatacaa cgccgcaggc gacctgactg ccgttatcac cccggacggc aaccggagcg
       61 agacacagta cgatgcgtgg ggaaaggcgg tcagcaccac gcagggcggg ctgacgcgca
      121 gtatggagta cgatgctgcc ggacgtgtca tcagcctgac caacgagaac ggcagtcaca
      181 gcgacttcag ctacgatgcg ctggaccgcc aggtacagca gcgcggcttt gacgggcgga
      241 cgcaacgtta tcattatgac ctgaccggaa aactcacgca gagtgaagat gaggggcttg
      301 tcatcctctg gtactacgat gaatcggacc gtatcactca ccgcacggtg aacggcgaac
      361 cggcagagca gtggcagtat gatggccacg gctggctgag agaaatcagc cacctgagcg
      421 aaggccaccg tgttgccgtc cactatggct atgacgataa gggccgtctg accggggagc
      481 gccagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag acgaaacagg
      541 catacaacga acaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt
      601 ggctgaccta tggcagcggt tatctggcgg gcatgaagct gggcgggacg ccgctgctgg
      661 agtacatgcg ggacagactg caccgggaga cggtgcgcag cttcggcagc atggcaggca
      721 atacagccgc atatgaactg accagcactt acacccccgc aggccagtta cagagccggc
      781 acctgaacag cctggtgttt gattgtgact ataactggaa tgacaacggc gacctgatgc
      841 gcatcagcgg cccgcgacag acgcgggaat acggctacag
//
LOCUS       DAZGAP010000489          876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGAP010000489 DAZGAP010000000
VERSION     DAZGAP010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.492"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>876
                     /locus_tag="ACHLAM_005481"
     rRNA            <1..>876
                     /locus_tag="ACHLAM_005481"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          221 a          197 c          291 g          167 t
ORIGIN      
        1 gcggacgggt gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg
       61 gtagctaata ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat
      121 cggatgtgcc cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc
      181 cctagctggt ctgagaggat gaccagccac actggaactg agacacggtc cagactccta
      241 cgggaggcag cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg
      301 tgtatgaaga aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt
      361 aatacctttg ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag
      421 ccgcggtaat acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag
      481 gcggtttgtt aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact
      541 ggcaagcttg agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga
      601 gatctggagg aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc
      661 gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg
      721 acttggaggt tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct
      781 ggggagtacg gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg
      841 gagcatgtgg tttaattcga tgcaacgcga agaacc
//
LOCUS       DAZGAP010000490          860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGAP010000490 DAZGAP010000000
VERSION     DAZGAP010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.493"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..291
                     /locus_tag="ACHLAM_005482"
                     /pseudo
     CDS             64..291
                     /locus_tag="ACHLAM_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
BASE COUNT          262 a          162 c          220 g          216 t
ORIGIN      
        1 ctgactaacc tgtatttcaa gattaaagaa gaccgtcgta aggcggcgcg gggagagtag
       61 acgatgaata aacaatacga actggttgtt aaagggataa ataattaccc ggataagatt
      121 actgttactg tggcactgga aattgccggg cagccgtcgt tgttgtcgcc atatgtggcg
      181 attagtcttg accgtactga aggtgccaag ctggaatttt acgaagctga ggcgaaaaag
      241 aaggcgaagc agtttttcat ggatattgct gccgctttat gtgaagggga tgaacagtca
      301 cgggaaaagc gcctctgttc agaagagcgg tataccatac aaattaacaa tgcatataac
      361 acgatacagt cagaaaaaga tgatatcgaa tcacgcattg aaaaactgga gaacagcgtc
      421 gttgaactga ataaaaaact gtcggtgcag atcccttccg aaggtgaaaa aaaacgccgt
      481 gatgagcagt tcgcggcgtt ttacgattat tgccggaaag tgatgagcag aaatctcgca
      541 gagtgtttcc gtattcaaaa aaacagagag cgctctgcaa atagtgagat tctggataat
      601 tttttaaagg ggacgcatct cgccagtgat tgtttcattg ctcattcttt tggtggcttt
      661 catgaggcca tcaaacatga tgttgagaga agtccaggta tcctctgttc cattcccgta
      721 agcatcgaaa tagacacaaa tacgataacc ggtgaactcg ttaccgccgg agtcggtata
      781 ttccgtcatg aggggaacga tgccaaaggt tcctttacaa aaatcgggcc gttctttctg
      841 aaggtagagc gatagtttgt
//
LOCUS       DAZGAP010000491          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGAP010000491 DAZGAP010000000
VERSION     DAZGAP010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.494"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>854
                     /locus_tag="ACHLAM_005483"
     CDS             <1..>854
                     /locus_tag="ACHLAM_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO7856820.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNLHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TQISHLSEG"
BASE COUNT          216 a          249 c          244 g          145 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacaccggat ggcagggcat cggcatttta ctataaccac
       61 cacagccagt taacgtcagc caccgggcct gacgggctgg aattgcgccg ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatctac acagtgactt accctgcgca acggaagatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacat gcagggccat
      421 gaaatgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggcttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac acaaatcagc
      841 catctcagcg aagg
//
LOCUS       DAZGAP010000492          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGAP010000492 DAZGAP010000000
VERSION     DAZGAP010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.495"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>854
                     /locus_tag="ACHLAM_005484"
     CDS             <1..>854
                     /locus_tag="ACHLAM_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO7856821.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGTDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TGISHLSEG"
BASE COUNT          212 a          250 c          249 g          143 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta ctataaccac
       61 cacagccagt taacgtcagc caccgggact gacgggctgg agttgcgcag ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatccac acagtgactt accctgcgca acggacgatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacac gcagggccat
      421 gaaacgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggtttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac aggcatcagc
      841 catctcagcg aagg
//
LOCUS       DAZGAP010000493          850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGAP010000493 DAZGAP010000000
VERSION     DAZGAP010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.496"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..386
                     /locus_tag="ACHLAM_005485"
     CDS             204..386
                     /locus_tag="ACHLAM_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7856822.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLIP"
     gene            424..669
                     /locus_tag="ACHLAM_005486"
     CDS             424..669
                     /locus_tag="ACHLAM_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7856823.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            746..>850
                     /locus_tag="ACHLAM_005487"
     CDS             746..>850
                     /locus_tag="ACHLAM_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO7856824.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQ"
BASE COUNT          226 a          190 c          247 g          187 t
ORIGIN      
        1 tgctgattga cagtgtgaag gtggaagtga acaacgcgga cgtcgcggca tgataaaaaa
       61 ggccgccagc aatgaaggaa agggatgaac tgtgtccact acatgaatgc tggcggctgg
      121 taaaacgacc atgatgacac cgaagtctgc ggtgtttttc tacaaaaata agtaaatcat
      181 gtcaactatc acggtgagaa accatgacat ttctgaacca gttaatgctg tacttctgta
      241 cggcggtctg tgtgctgtat cttctttcgg gtgggtacag ggctgtgcgc gatttctggc
      301 gcaggcagat tgataaaagg gccgcagaga aaatcagcgc cagtcagtca gccggaagca
      361 aacccgaaga gccgctcatt ccgtagtcac tttcttaaca acacctttca acgagaaaat
      421 cctatgtcag aaataaaatc gctggtcact gctgaagcag tgaaggaagt cctgcgctct
      481 gaagaagtca gaagcgcact gaaacagaaa ctccgccaga accttgagtc gcgtcttgat
      541 gcagaagtgg atgccattct ggatgaactg ctgggcgcac cggcagttcc tgagccggaa
      601 ggcattgcgg gtgacgggag tgcttcagat ggcggtgaac ccacacccga cagcgacatg
      661 atgatgtaag catgcgcaag ggaccatcgg tgtgtgtcgg tggtctttat attgttgtga
      721 gcttccggat tgcgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc
      781 atacaccacg tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc
      841 cccgtctcag
//
LOCUS       DAZGAP010000494          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGAP010000494 DAZGAP010000000
VERSION     DAZGAP010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.497"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHLAM_005488"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHLAM_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7856825.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
     gene            complement(369..>848)
                     /locus_tag="ACHLAM_005489"
     CDS             complement(369..>848)
                     /locus_tag="ACHLAM_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7856826.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT          209 a          238 c          215 g          186 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 cgcctgcctt acgcttttgc cttcggcatc tgcgaaacca gggacagact gacatccata
      421 ccttccacct ggaaatgcgg tacagcgtag agtttcacac ggaaaaatcc cgggttgtct
      481 tcgatatctt ccaccaccac ttttccgtca cgcagcggat gcgaggcctg caactcatca
      541 cccggatcgg tcatctccgt caccagaccc cggatccagt tattcagctc cagctcaagc
      601 agacggcgat ctttggtggt accgatattt tcccgctgaa tcatcttcag gtaatgcgca
      661 atgcgggaca gcaggaagat atagggaaga cgggcattaa tacggctgtt cgccgttgca
      721 tccggcgtat cgtacagtgc cggtttctgg gtggagttag cggagaagaa gcaggcataa
      781 tcccggttct tgtagtaaga gagcggaata aagcccagat tagcgaattc aaactcccgc
      841 gtttcagg
//
LOCUS       DAZGAP010000495          844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGAP010000495 DAZGAP010000000
VERSION     DAZGAP010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.498"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..>844
                     /locus_tag="ACHLAM_005490"
     CDS             75..>844
                     /locus_tag="ACHLAM_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO7856827.1"
                     /translation="MSIGADSVDKAATPVSGATIKLKNGGTIEYKGFAGNNAASVVSV
                     GDAGKERQIVNVGAGAVSEKSTDAINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSA
                     TINGGKDNKNGNTLTVKGDGDIAVTVNDKGMTIDGTTLKNEIKNDAIKIKGGDGKTLT
                     KSLGDNVEITGKGPIKTQVAGDKLEISAETASLPVNGGNGKITVNNGDGDKLVTAKNI
                     ADAINQSGWKIKADKDTGGVIEGNGQGTETLIKPGDMVT"
BASE COUNT          273 a          139 c          244 g          188 t
ORIGIN      
        1 gtcagagtac gacgggcaaa aagtccacgg ttgctctggg gcagggagct acagcctctg
       61 ccgcggaggg gaacgtgtcc atcggtgctg attcagtgga caaggcagca actccggttt
      121 ccggcgctac cattaagtta aagaatggcg ggactattga atacaagggc tttgccggaa
      181 ataacgcagc atcagtagta tctgttggtg atgcgggtaa agagcgtcag attgttaacg
      241 tgggggcggg tgctgtatct gagaaaagta cagatgccat taacggtagt cagttatatg
      301 cgatagcgaa gacactgaaa gatgaccttg atgccattaa tccgacgtgg acgctaaagg
      361 atgataaggg aggttctgcg actattaatg gtgggaagga taataagaat ggtaacactt
      421 taactgttaa aggtgatgga gatattgcag ttactgttaa tgataaagga atgacgattg
      481 acggaacaac gctgaaaaat gaaataaaaa atgatgccat caaaattaaa ggtggagatg
      541 gtaagacgct gacgaagagt cttggtgaca atgtcgaaat cactggcaaa ggcccaataa
      601 aaacccaggt ggctggagat aaactggaga tttcagcaga gacagcttcg ttgcctgtaa
      661 atggtggtaa cgggaagatt acagtaaaca atggtgatgg cgataaactt gttactgcga
      721 aaaacattgc tgatgccata aaccagtctg gctggaaaat taaagcggat aaagataccg
      781 gtggtgttat cgagggaaat ggtcagggaa cagaaacact gataaaaccc ggtgatatgg
      841 ttac
//
LOCUS       DAZGAP010000496          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGAP010000496 DAZGAP010000000
VERSION     DAZGAP010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.499"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..764)
                     /locus_tag="ACHLAM_005491"
     CDS             complement(<1..764)
                     /locus_tag="ACHLAM_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7856828.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNISYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     AQGICEITWQTLDIYNNVLPVWKVNMNPLQMDVSGS"
     gene            complement(730..>836)
                     /locus_tag="ACHLAM_005492"
     CDS             complement(730..>836)
                     /locus_tag="ACHLAM_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFO7856829.1"
                     /translation="VDNILHFFAAYKALKNNVQPGDANASVSFIVTYD"
BASE COUNT          254 a          168 c          157 g          257 t
ORIGIN      
        1 tgtgagcctg acacatccat ttgcaacgga ttcatattta ctttccatac aggcaataca
       61 ttattataaa tgtccaatgt ctgccatgtt atttcacaaa tcccttgcgc tttacaccat
      121 gattgttgac gatggctaaa tggtgcgatc ataccaccat ccataacttt accatcaaca
      181 tgaaggttat agagtgtcac atatctgggg cctggattat aaacggtaac aatttcattt
      241 tttcgtgccc agcgtaattt ttgatatgaa atattcgggt cttcagacaa atttgatggc
      301 cgatagaata ttttcatacg attgcggacc gctatttgca attgattatc agttttggtt
      361 ggatgactat tttccaacga tggtaatgca gcaacattaa gtataaataa actttcccgg
      421 tcggcaggta aactgccacc aacacgatta attcttattt ttgtattttt gtcaggcttc
      481 aaaacaaaca gtggcggagt tgcgataaat accgttccag aaaatgaggg caaagattca
      541 accgttcccg tcaactgacg agcatcatca ggggagactg caaccttgac taacatatct
      601 ctgtctgaag tgtttttgag ttcaacagat attgaggact gattttccgg atacgcaaaa
      661 cgcgttccac cgatcactaa tcctgcttgt gctgtagaga ataaaaggga aaataatact
      721 atgactacct taatcatagg tgacaataaa cgatactgac gcattggcat cccctggttg
      781 gacattattt tttaatgctt tgtaagccgc aaaaaaatgc aatatattat caactg
//
LOCUS       DAZGAP010000497          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGAP010000497 DAZGAP010000000
VERSION     DAZGAP010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.500"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          264 a          183 c          146 g          228 t
ORIGIN      
        1 ttatccaaac agccccatac ttcaggttaa aaagaacaat aaaaaatgat gctaaaattg
       61 caacattctc taaataaatt caattaatta tctaaacaag gtgaaacttt cagaggaaat
      121 gagcgcggcc ccccctaata atcttaagct gggtacaggg aagtatcgac cattacgcag
      181 tgtcgatatc gaatcgtaca aaaaacagtc ttggcgtggg atatttacac ataattatta
      241 gctcacaatc tccctatacc tgtcgtgttt tctggggatt cctcagggag tgaccataga
      301 ttccgttttc caggtgcccc cctttttctc caaaaattga aaaataactc tgtagatgcc
      361 gctaacaatc aattcgagtt gatattttaa agcgttctgt gcagaaagaa ataaatttca
      421 aaaatgaatc aggggagaaa agtgcgcttt ttaacctgtt cagtgtccag aacacatgaa
      481 accgatcgcc attcagtttc atgcatagtc atgcaacgca ctgaaaatga ttctgacgca
      541 tttttccggt ttcccccgag gaaaacatcc ctttttgcgt cagaatcgcg ctcagcgcgt
      601 tttagcggca cgtacaatta caggattata aatcgtcacc agcgactggc gtaaaaaagc
      661 ccctctctgc aggcgagatt tagatgtttt attaatcaca ccccaaagct gaatatttat
      721 cacaagccat ttttgccgac aaatcactaa ggcgatcaat gccaatggac ttacaataat
      781 aaacagattc ttttttgcta ttcaggctcg taacactaac t
//
LOCUS       DAZGAP010000498          798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGAP010000498 DAZGAP010000000
VERSION     DAZGAP010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.501"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..204)
                     /locus_tag="ACHLAM_005493"
     CDS             complement(<1..204)
                     /locus_tag="ACHLAM_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856830.1"
                     /translation="MKVCLYFISVFFSSCCIADVFHYRMPFRIQTYSPVTKSKIENFA
                     IKDGAQKLLQINREKLLMLLNRSS"
     gene            complement(262..>798)
                     /locus_tag="ACHLAM_005494"
     CDS             complement(262..>798)
                     /locus_tag="ACHLAM_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7856831.1"
                     /translation="YYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVSGFDPLG
                     LKVSFQGDESTQKTLKEAYKAVAETKFGHKITEELESSEHEYIFRGLRKGINQTCYDD
                     TEYSFYIDIDNDHSSCVYQGKNKACAMKPTLLSVVLAHEMGHAKGMKDDGTDSMANVD
                     KYENPFRKELGLPARMKY"
BASE COUNT          241 a          166 c          119 g          272 t
ORIGIN      
        1 actagacctg ttcagtaaca ttaataactt ctcacgattt atctgtaata atttttgtgc
       61 accatctttt attgcaaaat tttcaatttt acttttagtg acaggggaat atgtttggat
      121 gcgaaaaggc atcctataat ggaaaacatc agctatacag caagatgaaa aaaatacgga
      181 aataaaatac agacatactt tcataccatc tcctaaactg gtgatatatt aaaaacatac
      241 ccaaaagcaa tataaaaatg tttaatattt cattcttgct ggcaagccca actctttacg
      301 aaatgggttt tcatatttat caacattagc catcgagtca gttccatcgt ctttcattcc
      361 ctttgcatgc cccatttcat gcgcaagaac cacactcaac agagtgggtt tcatggcaca
      421 tgctttgttt tttccttgat atacacatga gctatggtcg ttatcaatat caatgtaaaa
      481 actatattca gtatcatcgt agcatgtttg gtttatgcct tttcttaatc ctctaaatat
      541 atattcatgc tctgatgact ctaattcttc tgttatttta tgtccaaact ttgtttctgc
      601 tacagcttta tatgcttctt ttaaagtttt ttgtgtactt tcatctccct gaaaagacac
      661 ttttaaccct aatggatcga aaccagatac cggattcaac ggatactgat aaaaattcca
      721 tccccccttc agcccaatcg gatcctgagt gatataccgc ccctgcagcg ggtcataata
      781 gcggtggcgg ttgtaata
//
LOCUS       DAZGAP010000499          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGAP010000499 DAZGAP010000000
VERSION     DAZGAP010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.502"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..254)
                     /locus_tag="ACHLAM_005495"
     CDS             complement(<1..254)
                     /locus_tag="ACHLAM_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856832.1"
                     /translation="MHHYKFFALIAMLLCGASFASDVYFLCNTEKGTIKLDENKGVLR
                     YTLSKDHKTEFNYESKGSDYSGFKYNHYSRFQTDYFNVSFV"
     gene            complement(241..>795)
                     /locus_tag="ACHLAM_005496"
     CDS             complement(241..>795)
                     /locus_tag="ACHLAM_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HFO7856833.1"
                     /translation="DDTDYEEAAKELGCEVAAIKAVAKTETGSYGSYFKFEDNDDYVP
                     AILFERHHFHKYTNGKYDQFEDISNPVAGGYGATSIQYAKLVKAYTLDKKAALKSASW
                     GKFQILASNYATAGYASPEDFVFALSKSEKNQLKAFVSFIKADRVLLRSIRTKDWLSF
                     AQRYNGPRQKGYDLKMERNYNASL"
BASE COUNT          234 a          168 c          122 g          271 t
ORIGIN      
        1 acaaacgata cattgaaata atcggtttga aatcgtgagt aatggttgta tttaaaccct
       61 gaataatcac tacctttcga ttcataatta aattcagttt tgtggtcttt cgataaggtg
      121 taccgaagta cacctttgtt ttcatctagt ttgattgttc ctttttccgt gttgcataaa
      181 aaatacacat cacttgcaaa cgatgcccca cacagaagca ttgcaatcaa cgcaaagaac
      241 ttataatgat gcattgtaat ttctctccat tttcaaatca tatcctttct gccttggtcc
      301 gttatatctt tgagcaaatg aaagccaatc tttagtccga atagagcgca gtaatactct
      361 atcagcctta atgaagctaa cgaatgcttt taactgattt ttctcagact tacttaatgc
      421 aaaaacgaaa tcctcagggc tcgcgtagcc tgctgtggcg tagttcgacg ctaaaatctg
      481 aaacttaccc caggatgctg attttaaagc tgctttcttg tctaaagtat acgctttaac
      541 tagctttgca tactgtattg atgttgcacc atacccaccc gcaacagggt ttgatatatc
      601 ttcaaactga tcatacttcc catttgtata tttatgaaaa tgatgccttt caaataatat
      661 cgctggaaca taatcatcgt tatcttcaaa tttaaaatag gagccataag aaccagtttc
      721 tgtttttgct acagctttaa ttgctgcaac ttcgcatcct aattcttttg ctgcctcttc
      781 ataatcagta tcatc
//
LOCUS       DAZGAP010000500          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGAP010000500 DAZGAP010000000
VERSION     DAZGAP010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.503"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..219
                     /locus_tag="ACHLAM_005497"
     CDS             <1..219
                     /locus_tag="ACHLAM_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7856834.1"
                     /translation="NPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAV
                     KGIAGIKVNKGAESFHVEPFEQDAGLNK"
     gene            304..>791
                     /locus_tag="ACHLAM_005498"
     CDS             304..>791
                     /locus_tag="ACHLAM_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFO7856835.1"
                     /translation="MSIKYRYFKMNERDSRHYHREWLDCAGRERKKLIDDFLSNHRAK
                     GYLYFWCCGHIFVTSILVHEDVDVGRNKRVLSDKFDEDGRVLFSVKPDRRFREGKKLN
                     KALNILNEKLKQLPSFSTWMVNKLDCYFEVFGVSNGRTVMACSSAGFYGDKGAVVVRI
                     PVG"
BASE COUNT          215 a          140 c          213 g          223 t
ORIGIN      
        1 aatccatcat gcagcgtcag tcgtgatgtg gaaggtgtga ttgaaatgct gcgtcgtatg
       61 gggcttgagc gcttcatccg cacgaaagag gaagtgaata agcaagctgt cctggcagag
      121 ccggatgcgg tgaaagggat tgctggtatt aaggtgaata aaggcgctga aagttttcat
      181 gtcgagcctt ttgaacagga cgccggactg aataaataac accgcattaa atctttaaat
      241 atcacatcgt tttaattatg gcgcttgcgt caggggactg ctcgcgcctg aatgaggcat
      301 atcatgagta taaaatatcg ttattttaaa atgaatgagc gcgattcccg tcattatcac
      361 agggaatggc ttgattgcgc tggcagggag cgcaagaaat tgattgatga ttttctcagt
      421 aatcatagag ctaaaggata cctttacttc tggtgttgtg ggcatatttt tgtcacatca
      481 atactggttc atgaggatgt tgatgtcgga agaaataagc gggtgctgtc tgataaattt
      541 gatgaagatg gccgcgtttt attttcagtt aagcccgacc gcagattcag agagggaaaa
      601 aaactgaaca aagcactgaa tatcttaaac gagaagttaa aacagcttcc gtcattcagt
      661 acctggatgg ttaataaact ggactgctat tttgaggttt ttggtgtcag caacggccgc
      721 acagttatgg cgtgctcttc tgccgggttc tatggcgaca aaggggctgt tgtggttcgt
      781 attcctgtcg g
//
LOCUS       DAZGAP010000501          789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGAP010000501 DAZGAP010000000
VERSION     DAZGAP010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.504"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>789
                     /locus_tag="ACHLAM_005499"
     CDS             <1..>789
                     /locus_tag="ACHLAM_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO7856836.1"
                     /translation="SGMINSKSLTDTYDVGLLDNQFDTPFSALSAIKPFIIIDEPHKF
                     PTGKKTWEHIQKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNEDLVKGIDAYIEDIV
                     GDGNANLKLVKSDGKEATFELNENNNKKIFKLAKGESLSKTHSAIHDLTLDALNKNTA
                     VLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKLEKEYLTQRPRIKPLTLFFID
                     DIEGYRDGNDIAGSLKTKFEEYVLAEANELLKTEKDEFYRNYLE"
BASE COUNT          277 a          133 c          147 g          232 t
ORIGIN      
        1 actcagggat gattaactct aagtctttaa ctgacactta tgatgttggc ttgcttgata
       61 atcagtttga taccccattt tctgcactta gtgcgataaa accattcatt atcattgatg
      121 agcctcataa atttcctact ggtaaaaaaa catgggaaca tattcaaaaa tttaatgccc
      181 aatatatcat ccgctatggc gcaaccttta gtgaaggcta taagaactta gtttatcgtc
      241 ttacagccgt agatgccttc aatgaagatc ttgttaaagg tattgatgca tacattgagg
      301 atattgttgg tgatggtaat gccaacctca agttggtcaa atctgacgga aaagaagcta
      361 catttgaact gaacgaaaat aataataaaa aaatttttaa gttagccaaa ggtgaatcac
      421 tatctaagac gcatagtgcg atccatgatt taactcttga tgctttaaat aagaatacag
      481 cagtgttgag taatggtatt gagctaaaaa ttggtagctc gataaacccc tattcttacg
      541 atcagacact tgctgataac atgatgcgta aagctgtcaa agaacacttt aagttagaaa
      601 aagagtatct gacacaaagg ccacgcataa aacctcttac tcttttcttc attgatgaca
      661 ttgaaggcta tcgtgatggt aatgatattg caggaagcct gaaaactaag ttcgaagaat
      721 acgttttggc agaagcaaat gaactgttaa aaacagagaa agatgagttt tacagaaact
      781 accttgaaa
//
LOCUS       DAZGAP010000502          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGAP010000502 DAZGAP010000000
VERSION     DAZGAP010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.505"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>788
                     /locus_tag="ACHLAM_005500"
     CDS             <1..>788
                     /locus_tag="ACHLAM_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7856837.1"
                     /translation="ALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWLP
                     EFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVKQ
                     KGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVID
                     GGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGIL
                     PRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGA"
BASE COUNT          191 a          206 c          229 g          162 t
ORIGIN      
        1 gcgcgttaag tgaaatcacg ctgaaacgct ggattgcggc ctttaacaag gcgcagaacg
       61 ccgctgaacg cctgctttta ctggcaccgg gtaagcgcca ggaaataaaa gccgaagata
      121 ttaactggct gcccgaattt ctggcgcagt atcgccagtc aaacggccga ccaatgaccg
      181 aggcttacga ggattttgtc gctgaatggc agcaccggca cgctgatgag ccttatatgc
      241 tcgatatcat gccgtcttat gacaccattc gccgcgcaat gaagaaactg ccggaagtgg
      301 tgaaacaaaa aggccgggtg accggcagtg aataccgcca gcttgaggga ttcacgcgcc
      361 gcgactggtc aaaaatgccg gtgaattatg tctggattgg tgacggtcac ggcatgaagc
      421 tgaaatgcgc acacccggtt cacggtcggc catttgcacc ggaagtgacc tttgttatcg
      481 acggtggcac gcgctttgtg gtgggctgga gccttgacct ggctgaaaat gttttcgccg
      541 tagccggtgc catacagcac ggcattcgcc atcacggcaa accgtttctg tattactcgg
      601 ataacggctc cggggaaacc gccgacatcc tggataagga ggttgtgggg atactgccgc
      661 gactggggat taatcacccg accgggattg ccggtaatcc gcagggacga ggcattatcg
      721 aacggcttaa ccgcacatta ccgatgcgca tagcccgtaa ataccgcacc tatttcggga
      781 aaggtgca
//
LOCUS       DAZGAP010000503          784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGAP010000503 DAZGAP010000000
VERSION     DAZGAP010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.506"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(135..698)
                     /gene="ipbA"
                     /locus_tag="ACHLAM_005501"
                     /gene_synonym="fimX"
     CDS             complement(135..698)
                     /gene="ipbA"
                     /locus_tag="ACHLAM_005501"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO7856838.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKCRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          235 a          155 c          159 g          235 t
ORIGIN      
        1 aaatcatggg gttattttac tttagcgaga aatatcaaca tgttatgtgt tttatttaat
       61 tgaatgagct gtttttttat ggaagaattg aggcaaacgt aggaatctga cattttgaat
      121 cagaaggtac tgttttaacg aggctttttt ttccacaccc ctttaaatcg agcagcatta
      181 ctggctgtgt atctgacagt atgctgaata tttcgatgac ccagataatc ctgaagtagt
      241 cgggtatcga caccattgtc agccagagca aatccacagg catgacgtaa catatgagga
      301 tgtgctttta cagctaatcc tgcacgtctt cctgcctcac gaatgatcga aaaaaaatgt
      361 tgtctgctga tagggcggca tttccgggag agaaaaagcc agtctccttc aactccattt
      421 tcaattaact tcctctgctt aagccacaac ttgatcaaat tatattcatc aggaagaaga
      481 ggatgtgttg tcgagaagcc atttttaata cgccggatat taagttgttt tcctgatgca
      541 tcaatatcgg ataaacgtaa atcaagaagt tcactggccc taaaaccgtg aatgaaagcc
      601 atcataatta gacagcgatt tctttcagga aaggacatgc tctgggctgc atccatcagc
      661 ctgtagactt catcctgtgt gagatatttt ctcgtcatac gccaccgtgt acagttgtaa
      721 aaaaaagagg cacacccaga taagtgtgcc tgcaatcaga acgttatcga tgtaatttca
      781 ttat
//
LOCUS       DAZGAP010000504          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGAP010000504 DAZGAP010000000
VERSION     DAZGAP010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.507"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            343..>781
                     /locus_tag="ACHLAM_005502"
     CDS             343..>781
                     /locus_tag="ACHLAM_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO7856839.1"
                     /translation="MARLLTIVLFFSISANAVTVNQFQRELDLADKTILWALISASTQ
                     EGRKACSLSYFSCKAAEAELGLAYMAANDNKAFLTSLSRIMMYKIDAGLSESYTCYLL
                     SKGKIIRPYLKNLNPHQLVADCIETVNKIKDKNKKIIDIDSVNI"
BASE COUNT          274 a          136 c          153 g          218 t
ORIGIN      
        1 gagcggaaaa ccagcggtat actaaggcga tgccatgttg aaagatagtc caatagtcta
       61 aagtggagaa ctcaatgaag caaaccggac gaggcttgct taacagtcct ggagcaacca
      121 atagaaaacc taatggtata cgtttagtac cgtcagcaaa tacagaaacg tataatcgtg
      181 atggattttt aacacatagt tgtttaaacg cttttggtcc atctttagga cctcgattct
      241 gttccgaggg ttgtataact ggttcatcta atgacatgca aaaattaaat gagctaatat
      301 tttcagagcc agatagcaca ttaacagtga cggactagaa atatggcaag gttattaaca
      361 attgtgcttt ttttctctat ttctgcgaat gcagttactg taaaccaatt tcagagagag
      421 ttggatttgg cagacaagac aatattatgg gcactaatat cagccagtac ccaagaggga
      481 cgaaaagcgt gttcacttag ctatttttca tgtaaagccg cagaggctga actcggactg
      541 gcatatatgg ctgctaatga taacaaggca tttctcacat ccttatctag aataatgatg
      601 tacaaaatag atgctggact ttccgaatcc tatacatgct atttattaag caagggaaag
      661 atcataagac catatctgaa gaatctaaat ccacaccaac ttgtcgctga ttgcattgaa
      721 actgtaaata aaataaaaga taaaaataaa aaaatcattg atatagattc agttaatatt
      781 t
//
LOCUS       DAZGAP010000505          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGAP010000505 DAZGAP010000000
VERSION     DAZGAP010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.508"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          277 a          136 c          151 g          214 t
ORIGIN      
        1 aaaagttacc aaatcttagc acctcagtca ggagagtcag gtcatcgata aagaatattc
       61 cagtagagtg gcgctcatga tcaaagataa cagcaaagtt gatgacagcg ctgaccgact
      121 gtcatttaca gaaaccctgt ttgtagcaca ttccatctta cctgacccag acaaagacaa
      181 agacaaaaga gaatgattgt tatttacttt tcaaaaaaaa gtgtcatatt gtccatataa
      241 agtcaagagg tattttacgt tatgtcactg atgcaattgt tagtcaggat ctcatggttg
      301 tcgctcatgt ataacctgag acttgaacgc acactaatag cactgacggt catacatcgg
      361 gaaatgtgag gaggcataag aatgaattgt gataataatc acagaaatga agaattcatt
      421 gttacctttg ataaaggcaa caagcaagac aattcaagac gaaaacacga taattttcct
      481 atagaggtag aatcctccgt agagcaggag acacactgta tcacaaataa taagtcggct
      541 tccggtatag taacacatga ctatgatgcc gattatattt gtggttgtgg tgaaattatg
      601 tgtcctggtt gcggtcatga cctataacaa acatttttgc aaactgatac ctgatcaggt
      661 acaagcctgt tgattattat gctgaatatt tcatgttata aaaccatcga tataaacatt
      721 aacaagaaaa aacatatatt gtttatctga acatttatcg atggaaaacc aaagggga
//
LOCUS       DAZGAP010000506          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGAP010000506 DAZGAP010000000
VERSION     DAZGAP010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.509"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          210 a          149 c          173 g          242 t
ORIGIN      
        1 agtgatgccg atcgtgtttc tgtgctcttt tctgataaga gtaatttttc agatttccgg
       61 aagtccgtat tttgcacggg cccggttgta tgcttcagac tgcggtggat atcaaggtca
      121 cggagttggt cggttgcaag gaatagattg agaattgagc gtgtttctgg cacatgcctg
      181 actgtaatca tccctacaaa ctacattgat gccagaaacg cactggtaac agggcatcgt
      241 gtatttcagt atgtccagtt tttgttgcgg tgattgcata aaaaatcccc cttgaggggt
      301 gaaatatcac cggaattttc ggcaagtatt tttatcgggt tgagtgtatt tctggggctc
      361 tgctgaggca attcactgaa tgtggctttt gtgctcgctg gaaatgcact catacacttt
      421 ttaacgcgat ttttttcagg caatagtttt taatttgtta gcgttctcgc tgtcaggcac
      481 gttttgagca cgtttccgac tctcagctac agcaatccac gactagggac agcacttacg
      541 ttttacttag cgcaccgtaa aactccgctc ttcagggtaa gggcttacat gaagaaaaaa
      601 aataaacttg tgaatatgaa agtaaatact ataaaaagat ggttcctctt ttttctcacg
      661 tgctgaataa tataaacaat agtgcatgat acaaaagaca ctaataacca caaagttatg
      721 atacggagca cgatttatgt cttcctgatt cagtgtgtca agaaacagtt atca
//
LOCUS       DAZGAP010000507          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGAP010000507 DAZGAP010000000
VERSION     DAZGAP010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.510"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>774
                     /locus_tag="ACHLAM_005503"
     CDS             <1..>774
                     /locus_tag="ACHLAM_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO7856840.1"
                     /translation="SVRGSSTVQPGAPVSGIRSEVPECQPDTDTEAMPESTVSIFPGT
                     ERKQHGITRPLKAVSDADTERTEKCSRPSQEESAGRGDSGASSAENALRPEIQPDMYG
                     GKPVFSGDVMEDGAAGAVLASVMTGDTAPGTTDALPASVTHFAVGRDDIEHLQNEAFR
                     LAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFLAGLAGDAPRTVLPVSLK
                     DLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSV"
BASE COUNT          162 a          197 c          255 g          160 t
ORIGIN      
        1 ttcagtccgt ggcagcagta cggtacaacc cggggcaccg gtatccggga tccggtctga
       61 agtaccggag tgtcaaccgg atacggatac agaggcgatg ccggaaagca cagtctccat
      121 attccccggt actgagcgca agcaacatgg tattacccgg ccactgaaag ccgtttctga
      181 tgctgacaca gagcgcactg aaaaatgcag tcgaccttct caggaagaga gcgcaggcag
      241 gggggacagt ggggcgtctt ctgctgaaaa tgccctgcgt cctgagatac agccggatat
      301 gtacggtggt aagccagtct tcagtggtga tgttatggaa gatggtgctg ccggagcagt
      361 actggcatca gttatgacag gagatactgc cccgggaacc actgatgctc ttcccgcgtc
      421 ggtgacgcac tttgctgtgg ggcgggatga tatcgaacat ctgcagaatg aagcgttccg
      481 tctggcctgg gaactggctg aatcagccgg ttgtgctgaa gagattgcca tggacaggga
      541 aagcgacctg tctgccggtt tcggggcagc agtggagaaa tgttctcctg tagtggcgtt
      601 tctggcgggg ctggccggtg atgcaccgcg gacagtcctc cccgtgagtc tgaaagacct
      661 gcttacaggg ggcagtgcac cggaagcgtg gccactgctg gatgacgacc atgccgtgaa
      721 gctgctgcgt cttctgagtg tgctgcgccg tctgcgagcc ctgcagcgca gtgt
//
LOCUS       DAZGAP010000508          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGAP010000508 DAZGAP010000000
VERSION     DAZGAP010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.511"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>774
                     /locus_tag="ACHLAM_005504"
     CDS             <1..>774
                     /locus_tag="ACHLAM_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO7856841.1"
                     /translation="SVRGSSTVQPGAPVSGIRSEVPECQPDTDTEAMPESTVSIFPGT
                     EREQHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGASSAENALRPEIQPDMYG
                     GKPVFSGDVMEDGAAGAVLASVMTGDTAPGTTDALPASVTHFAVGRDDIEHLQNEAFR
                     LAWELAESAGCAEEIAMDRESDLSAGFGATVEKCSPVVAFLAGLAGDAPRTVLPVSLK
                     DLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSV"
BASE COUNT          164 a          196 c          254 g          160 t
ORIGIN      
        1 ttcagtccgt ggcagcagta cggtacaacc cggggcaccg gtatccggga tccggtctga
       61 agtaccggag tgtcaaccgg atacggatac agaggcgatg ccggaaagca cagtctccat
      121 attccccggt actgagcgcg agcaacatgg tattacccgg ccactgaaag ccgtgtctga
      181 tactgacaca gagcgcactg aaaaatgcag tcgaccttct caggaagaga gcgcaggcag
      241 gggggacagt ggggcgtctt ctgctgaaaa tgccctgcgt cctgagatac agccggatat
      301 gtacggtggt aagccagtct tcagtggtga tgttatggaa gatggtgctg ccggagcagt
      361 actggcatca gttatgacag gagatactgc tccgggaacc actgatgctc ttcccgcgtc
      421 ggtgacgcac tttgctgtgg ggcgggatga tatcgaacat ctgcagaatg aagcgttccg
      481 tctggcctgg gaactggctg aatcagccgg ttgtgctgaa gagattgcca tggacaggga
      541 aagcgacctg tctgccggtt tcggggcaac agtggagaaa tgttctcctg tagtggcgtt
      601 tctggcgggt ctggccggtg atgcaccgcg gacagtcctc cccgtgagtc tgaaagacct
      661 gcttacaggg ggcagtgcac cggaagcgtg gccactgctg gatgacgacc atgccgtgaa
      721 gctgctgcgt cttctgagtg tgctgcgccg cctgagagcc ctgcagcgca gtgt
//
LOCUS       DAZGAP010000509          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGAP010000509 DAZGAP010000000
VERSION     DAZGAP010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.512"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..148
                     /locus_tag="ACHLAM_005505"
     CDS             <1..148
                     /locus_tag="ACHLAM_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856842.1"
                     /translation="AVINARRREMGQKPLTTAQIVDEICDFVANQQAVFLGGHYILQG
                     SRNR"
     gene            162..463
                     /locus_tag="ACHLAM_005506"
                     /pseudo
     CDS             162..463
                     /locus_tag="ACHLAM_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(463..558)
                     /locus_tag="ACHLAM_005507"
                     /pseudo
     CDS             complement(463..558)
                     /locus_tag="ACHLAM_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          137 a          244 c          238 g          150 t
ORIGIN      
        1 agctgtgatt aatgcgcggc gtcgtgaaat ggggcagaaa ccgctcacca ccgcgcagat
       61 tgttgatgaa atctgtgatt ttgtggcgaa tcagcaggcg gttttcctcg gtggtcacta
      121 tatccttcag ggcagcagaa acaggtgata cagggcaggc ggtggaggcc gcctgtcagc
      181 ccgcgcgaaa acccctggca ggctggcgcc tgccggaagg gaagcgggcc acgctggcgc
      241 gtcgcccgtt acccttcctg ttggtgacag gcgttgtttc aggaagtacc tgttacagac
      301 ctgtcgttaa agaaccttct tacacatgcg ccccttcggc tcccggctgc ctggcggcat
      361 ccggtcgtcg gcccgactac gacaggcagc tgctggcgca cccgccagtc tccgccgaac
      421 ccgctggcgc ggtttcgggc tgtcgctacg gtccgatgcg tgaaaacccg ggcttgcagc
      481 gaccggaacg gtcgcgccgc ctttgtcgcg ccgtggcctg cgctcctggc atcccggcaa
      541 aaggccgccg ggatgtaagg aaaccgcgcc tgatggcgcg gcagtctcct cttctgccgc
      601 cacctctgcg gcctgtttcc cgctgtggac tttccggggg gcagacgtaa ccacctcccg
      661 ttccatcagt ccaggggaag atataccggc cacaaaattt ttagccatga acccggcaaa
      721 aaattttgcg tccgcccccg gacagctgtc ccgtgaggcg gtaaacggg
//
LOCUS       DAZGAP010000510          764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGAP010000510 DAZGAP010000000
VERSION     DAZGAP010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.513"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..613
                     /locus_tag="ACHLAM_005508"
     CDS             <1..613
                     /locus_tag="ACHLAM_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO7856843.1"
                     /translation="PDTVSQRDASTTQKGVVQLSSDTGSNDETKAATPKAVKAAMDVA
                     NEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGETGATGPAGPRGAQGPKGEKGDPGP
                     RGERGETGPMGPPGASDGKSRVVGIRLGNEQTYRAEESSGFLSISLSIGGVITGLKSR
                     GADGVISVSYRPLQVTFDGSQWRTVSVGEYVSSGASGFEYFPL"
     gene            655..>764
                     /locus_tag="ACHLAM_005509"
     CDS             655..>764
                     /locus_tag="ACHLAM_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7856844.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSL"
BASE COUNT          206 a          153 c          264 g          141 t
ORIGIN      
        1 accggatacg gtcagccagc gggatgccag cacgacgcag aaaggtgtgg tgcagttaag
       61 cagtgatact ggcagtaatg acgaaacaaa ggcagccacg ccgaaggccg tgaaggcggc
      121 aatggatgtg gcaaatgaag cgaaaacaaa ggcagaagag gctgcagcag gaggtggtgt
      181 tcccggtccg aaaggagaga aaggcgaccc cggagcacag ggtccgaaag gtgaaacggg
      241 agcaacaggc cccgctggtc cgcggggagc gcagggaccg aaaggggaga aaggcgaccc
      301 cggtcccaga ggtgagcgtg gagaaaccgg gccgatgggg ccgcccgggg catcagacgg
      361 gaagagccgg gttgtgggta tcaggctggg taatgaacag acttacagag cagaagagag
      421 ctctggtttt ctgtcaataa gcctgagtat aggaggtgtg attactggtc tgaaaagtag
      481 aggagcagat ggtgtgatat cggtttctta caggccgctt caggtgacat ttgatggttc
      541 tcagtggcgt acagtcagcg tgggtgagta tgtgtcgtca ggagcttcgg ggtttgagta
      601 ttttccatta taaagcgcat catctttcag acatgtaacc gggaggactt acagatgcac
      661 ataaagaatt tcagacaata cacgccggaa aatccggatg tgccgggtgc gatgtacctg
      721 aaatcagagg atggtcagga ctggtatgag tgtcagtcgt tatt
//
LOCUS       DAZGAP010000511          762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGAP010000511 DAZGAP010000000
VERSION     DAZGAP010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.514"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>762
                     /locus_tag="ACHLAM_005510"
     CDS             <1..>762
                     /locus_tag="ACHLAM_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO7856845.1"
                     /translation="TLFETITLFDDFSADDMQYGDMEEQDFLSLGLSDISAKVDPYRL
                     IKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFSFFGQYKTLIQDLI
                     DHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDISSTPTIGYRPLLLQ
                     KIKTELLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQD
                     HFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKP"
BASE COUNT          254 a          136 c          121 g          251 t
ORIGIN      
        1 tacacttttc gaaactatta ctttattcga tgatttttct gctgatgata tgcaatatgg
       61 ggatatggaa gaacaagatt ttctctcact cggcttgagc gacatctctg caaaagttga
      121 tccttaccgc ctcattaaat accattttcc tgggccgaat tctacttatg gtgcttttag
      181 tgttccaact tctggaacaa aaatatcaca aagtgaatgt attgatattt tatttgcaga
      241 aatgaaagat ctggcaaaaa tgttttcctt ttttggacaa tacaaaacac tcatccagga
      301 tctgattgat cattttagat atggaaatgg caacagtttc catagccagg aattgaactt
      361 atcttttcat gaacgaataa acaaatatga ttataatagc ccaatacgtg taatcaaaga
      421 atgtatcgaa aatgatattt cttcaacacc tacgatagga taccggcctc ttctcctaca
      481 aaagattaag accgaattac tttcatcaag actaaataaa tttaatgact tcgaggacag
      541 ttttaatggc ttaggaatca gtgttcatga tatttcagct caaaaaatat ctcttttaag
      601 ttttcaaaaa tacgctatag gatggagtgc aacaattcat tttgtagcac aggaccactt
      661 cggtcttgat gtaactgata tcaaaaacaa gacttatagc aaatatagat tttttcgcat
      721 atggttcttt ttacaacggc ataaagactt tgctttcaaa cc
//
LOCUS       DAZGAP010000512          758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGAP010000512 DAZGAP010000000
VERSION     DAZGAP010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.515"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..>758
                     /locus_tag="ACHLAM_005511"
     CDS             43..>758
                     /locus_tag="ACHLAM_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7856846.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          253 a          132 c          164 g          209 t
ORIGIN      
        1 atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg taaatgggcc
       61 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
      121 ggtgtagaaa tctatgctgc tgccaaacaa ggtgaacccg atccagaatt aaacacatct
      181 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      241 gcaattgata aagccaaagg cggcggagat gaaacgttcg tacagggacg ttatgaaggc
      301 tttggtccta atggctcaat gattattgct gagacattga cttcaaatgt taaccgtacg
      361 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      421 gtcagctata tgtttgacaa tacgggtgtg attgtattta aaggaacaga ccctgaccat
      481 atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga agaagaaggt
      541 aacattgtta tttatactga acctaccgac cttcataaag gaatcgcggc tctaaaagca
      601 gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc tgaagttgag
      661 ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatgacgac
      721 gatgtacaaa aagtttatca taacgtcgca aatctcta
//
LOCUS       DAZGAP010000513          757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGAP010000513 DAZGAP010000000
VERSION     DAZGAP010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.516"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..450
                     /locus_tag="ACHLAM_005512"
     CDS             <1..450
                     /locus_tag="ACHLAM_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7856847.1"
                     /translation="RGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAV
                     FRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRSQAR
                     EQFTGAVDRIFDTAESVCVASVVRTALRAVRNTVVSVARAVWNWIFF"
BASE COUNT          165 a          211 c          195 g          186 t
ORIGIN      
        1 cggggacatc agcaggtgct gtttgtggtg acgcaggccg acaaaacgga gccctgccat
       61 gaatgggata tggccggtat tcagccttct cctgcacagg cacagaatat tcgcgaaaaa
      121 acggaggcgg tattccgtct gttccggccc gtacatccgg ttgtggccgt atcggcccgc
      181 accggctggg aactggatac gctggtcagt gcgctcatga cagcgcttcc cgaccatgcc
      241 gccagtcccc tgatgacccg actgcaggac gagctgcgca cggaatctgt ccggtctcag
      301 gcccgtgaac agtttaccgg tgcggtggac cggatatttg acacagcgga gagcgtctgt
      361 gttgcctctg ttgtccgtac ggccctgcgc gctgttcgta acacggtggt ctctgttgcc
      421 cgtgcggtat ggaactggat tttcttctga acctgtcgtg actgatgccc tccctgactc
      481 tgagtctgct cacaaaagcg ctgttttcgt tactgtctct cttgtccgtg caatagccaa
      541 acaatagaat aaaacgatcg atacctattt tatcgatcgt ttatatcgat cgataagcta
      601 ataataacct ttgtcagtaa catgcacaga tacgtacaga aagacattca gggaacaaca
      661 gagccacacg tcagaaactt ccgtcagccg gacctctggc actgtaaccc tttacctgcc
      721 ggtatccaca tctgtggata ccggcttttt tattcac
//
LOCUS       DAZGAP010000514          750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGAP010000514 DAZGAP010000000
VERSION     DAZGAP010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.517"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..168
                     /locus_tag="ACHLAM_005513"
                     /pseudo
     CDS             1..168
                     /locus_tag="ACHLAM_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            250..>750
                     /locus_tag="ACHLAM_005514"
     CDS             250..>750
                     /locus_tag="ACHLAM_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO7856848.1"
                     /translation="MLWTENDAANTSQWNGYPLQIGRFRKDKAMPALISGEKSAALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSVIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIK"
BASE COUNT          302 a          132 c          126 g          190 t
ORIGIN      
        1 acaagcactg acagtacgtt atttaatgaa aaattcccat ttcaactaag gcaagatttc
       61 gcatcaagaa taacagaaga tgaaagtgat ttttttaaat ggaaaaaaat aagagagttg
      121 gcatttgtga gcaatcataa agaatggaat aaatatgact tattaatttt aaaatctgtt
      181 aatgaaatca acattcattt gtccagcact ccatactttc agccgttgga ttggtatata
      241 ataaaagcca tgctttggac tgaaaacgat gcggcaaata cttctcagtg gaatggttat
      301 ccattacaaa ttggtcgttt tcggaaagat aaagcgatgc ctgctttaat ctctggagaa
      361 aaatccgcag cccttgttac cccaccacaa tggagaaaca aagcattcaa tgggttaaaa
      421 gatccagaaa gaaattactg ggcaaaagaa caaataactg gctctccaga agagaatatc
      481 aaagcagcaa ttacttactt aatgatgaaa ctttctaata caaaggaaga atcaactatc
      541 gatcaatatg actcaactct atatagcgtc atcgtccaaa aaggcgattt ggctgacaac
      601 atcagaaaag agagaaaaac gaccatacct aacttaacga aaaacaaccc tggaaaaaat
      661 ctcgataaaa tacacccggg agatatttta tattatcaaa aagcatccat gaaagtaata
      721 attactggat ggaaacctat cacaataaaa
//
LOCUS       DAZGAP010000515          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGAP010000515 DAZGAP010000000
VERSION     DAZGAP010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.518"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..647
                     /locus_tag="ACHLAM_005515"
     CDS             180..647
                     /locus_tag="ACHLAM_005515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856849.1"
                     /translation="MKGKIIPEKNKIKNLINQERDAIALWIKDGMRLPDIYRLLKEKH
                     PELTFSQNGFVFSLRRDASDLHENALFNRSTVAVFMQKQHSEMTLMVNSGCLLKNVHE
                     ALFSHISYSVFLRYIVKLYPDLHYQAKLNRKGAVCGELHHEENASVYQNDEVC"
BASE COUNT          222 a          111 c          166 g          247 t
ORIGIN      
        1 tcatggcagc ctcatgtatt tatgtggaga ttatttcatc ggatattatt ttatccagga
       61 atgttgtttt gttaaagtga gtaagtctta tagattatgt ttctttactg aattccgggt
      121 atgtagacaa agctggttct gtatatgtga acttctttgt tgttcctgag ggagataata
      181 tgaaaggcaa gattatacca gaaaaaaata agataaaaaa cttaatcaac caggagcgtg
      241 acgccattgc actctggatt aaggatggaa tgagactgcc agatatttac agattactga
      301 aggaaaaaca cccggaactg acgttttcac agaatggttt tgtattttca ctgagacgtg
      361 atgcaagtga tttacatgaa aatgcgcttt ttaatcgcag tactgttgct gtatttatgc
      421 agaaacaaca ttctgagatg actctcatgg ttaattctgg ttgtttgctg aaaaatgtgc
      481 atgaggcgtt attcagtcat atatcttact ccgtgtttct tcgatatatt gtgaagttat
      541 atccagattt acactatcag gcaaaattaa acaggaaagg ggctgtctgt ggtgaattac
      601 atcatgagga gaatgcttct gtttatcaga atgacgaggt ttgttaaagg tgttttataa
      661 aaatagtggg gtagtgctgg cacagagatg tcttacaggc attctcccgc aaaaaatgaa
      721 cccgttgctt gttatagtgc cttgct
//
LOCUS       DAZGAP010000516          743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGAP010000516 DAZGAP010000000
VERSION     DAZGAP010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.519"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(309..>743)
                     /locus_tag="ACHLAM_005516"
     CDS             complement(309..>743)
                     /locus_tag="ACHLAM_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7856850.1"
                     /translation="VLFVVTQADKTEPCHEWDMAGIQPSPAQEQNIREKTEAVFRLFR
                     PVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDGLRTESVRSQAREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          184 a          194 c          210 g          155 t
ORIGIN      
        1 gtgaataaaa aagccggtat ccacagatgt ggataccggc aggtaaaggg ttacagtgcc
       61 ggagatccgg ctgacggaag tttctgaatt gtggttctgt tgttccctga atctcatgtc
      121 tgtgtgtatc tgcgcacgtt gctaacagga gttattatta gcataacgat cgatattaac
      181 gatcggtgaa atgggtatcg atcgttttat tctattatct agctattgca cggacaagag
      241 agacagtaac gaaaacagcg cttttgtgag cagacccaga ggcagggagg acatcatcca
      301 cgacaggttc agaagaaaat ccagttccat accgcacggg caacagagac caccgtgtca
      361 cgaacagcgc gcagggccgt acggacaaca gaggcaacac agacgctctc cgctgtgtca
      421 aatatccggt ccaccgcacc ggtaaactgt tcacgggcct gagagcggac agattccgtg
      481 cgcagcccgt cctgcagtcg ggtcatcagg ggactggcgg catggtcggg aagcgctgtc
      541 atgagcgcac tgaccagcgt atccagttcc cagccggtgc gggccgatac ggccacaacc
      601 ggatgtacgg gccggaacag acggaatacc gcctccgttt tctcacgaat gttctgttcc
      661 tgtgcgggag aaggctgaat gccggccata tcccattcat ggcagggctc cgttttgtcg
      721 gcctgcgtca ccacaaacag cac
//
LOCUS       DAZGAP010000517          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGAP010000517 DAZGAP010000000
VERSION     DAZGAP010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.520"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..679)
                     /locus_tag="ACHLAM_005517"
     CDS             complement(<1..679)
                     /locus_tag="ACHLAM_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO7856851.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFIYPRLYIARELLREDGVIFISIDENELNQLKTI
                     CDEIFGEA"
BASE COUNT          216 a          144 c          146 g          235 t
ORIGIN      
        1 ttgcttcgcc aaaaatttca tcacaaatag ttttaagttg atttaattca ttttcgtcaa
       61 tcgaaataaa tatcacacca tcttcacgta gaagctcacg agcaatatat aaacgaggat
      121 aaatgaacgt taaccatgcg ctatggctgc ttgaaccatt cgtggtaaat tcaagtatac
      181 gtttagcctc atcaagatcg atacctgcca gatcagaaag ctgttctggt gtaaatttgc
      241 gatcatcatt ataggcgaac ccatcagaac ccgtgttata tggcggatcg atataaatca
      301 ttttaacctt ttcagaataa gcattaacca tatgctttaa tacctcaagg ttatcccctt
      361 tgattaacag attttgactg ttcttgttct cttcacgtct gttatggtct ttatcttctg
      421 acagcaatgt cgttggtggt aggttagcca gcaggcgagc ataggatttg ccaagccagt
      481 ttaagcagta tgactcttta gagatttcaa catttgatgc tctgatgatc tcaagcagct
      541 tttcctgaat gaaagctccg tttttatcga aacactgtgg aaagttagtt ttcaaaacag
      601 caagttgttt actgttcgca gtttcagttt cagaaaaaag agtttctgtt cccggtagat
      661 gattgttgcg tatattcata cagcacctcc agaaagcacg gaggtagagg tacttgacgg
      721 agagagttca ggcgagcata t
//
LOCUS       DAZGAP010000518          736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGAP010000518 DAZGAP010000000
VERSION     DAZGAP010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.521"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..248
                     /locus_tag="ACHLAM_005518"
     tRNA            173..248
                     /locus_tag="ACHLAM_005518"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ile,seq:cat)
BASE COUNT          206 a          144 c          173 g          213 t
ORIGIN      
        1 taacatgctt gttgactgcg agtatcgact ttgcgaatgg aagcggtgtc gggatttatg
       61 tcgcatgtat tgacgaccca ggatggatga gaacgcataa aaatctgtta tttacatcat
      121 aagatcatca tgtttattgc gtggtttata agtattgtta ttgcatactt aaggcccttt
      181 agttcagtgg ttagagcagg cgactcataa tcgcccggtc gctggttcaa gtccagcagg
      241 ggccaccaaa tcatcattca tgtatcaaga agagttaagg gtacaagggg tactcgatgg
      301 cggtctatat ttcatgacta acgccattta aaggaagaag tgcggtgggt ggtttcgaat
      361 gagggctggt gttttggtac gtggcacgcc actgcaatgt atttatccat cagtactcat
      421 tcaaaagtac ctggaaaaaa tgcccgctat gccaagcggg caaattgctt acatgtggaa
      481 ttaagtaatt aacgataaag ccagagtatt atcatttatc atgcctcttc atttaatcag
      541 gttaattact caaatcactg aactattctc tgttcgctca ctgatgataa gtgaagtgtc
      601 acaaccacga tttgtatgtg gaccaatgcc gtagagtaag gatgaaatgt ctctgccacg
      661 ccttaaatat ctactactga ccagaacacc cgccgcaggc agatgagtct tatgagcgtt
      721 acaggagtct ggcttt
//
LOCUS       DAZGAP010000519          734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGAP010000519 DAZGAP010000000
VERSION     DAZGAP010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.522"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            146..>734
                     /locus_tag="ACHLAM_005519"
     CDS             146..>734
                     /locus_tag="ACHLAM_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856852.1"
                     /translation="MSPDIISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQE"
BASE COUNT          231 a          150 c          143 g          210 t
ORIGIN      
        1 aggtatgact tagcgggagt gattgtttct gagtgaacag agaccacttt cgcctgataa
       61 atgaaaaatg agcgattttt gcaagttagt ttttctatag ccgagtacgc tcagcgcctc
      121 gcttgattac aatgaggttg ctgctatgag tcctgatatt atcagttctg ctgtcaatcg
      181 cttctcaggt tggccatcta atgatcctct aaaaacagat aaagaatcaa tgacatcacc
      241 catgactgtg caggtaataa agatgattgc cgccatcctt gacccacatc ggatagtcgt
      301 tggaatcgat acactttata caacgcagcg cgaaacattt acctcagaga gtcaacagct
      361 cattgaatca gcattgcaaa cacttcatgt gatgaattta aataacctgg aaacaaatct
      421 cgatgaggaa ggtcactatc tggcttttgg atataacgtt gacactcctg acagaataac
      481 cgtttctgct tataatgaaa aaaagcctgg ctgctatggc caatgtcttt taatacagga
      541 tgacgaaaca gacttattta acctgataaa agttaaaatt tttgaagcca ggcataatta
      601 tgtcgctcaa ttgatgagcg atccggaatt tatgttcaaa tttagttata gcgctcaaca
      661 aaatagatta gaacctcatc ttctggttcc cacttatttc cctctaaaaa caaacgaacc
      721 ggttacgcaa gagg
//
LOCUS       DAZGAP010000520          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGAP010000520 DAZGAP010000000
VERSION     DAZGAP010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.523"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>732)
                     /locus_tag="ACHLAM_005520"
     CDS             complement(<1..>732)
                     /locus_tag="ACHLAM_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856853.1"
                     /translation="YMMNSYPVYSTLKSEYIRNYFLKSFGVVFIVLDIYNTYRPENET
                     GFYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGFNREDFRENGKMYELG
                     KYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELDRILNNIDKFYYAFYS
                     LSILLDMQRKVKILDSIAYYLRELIREGLWVHGLYGYAARYLYDFNIFDTTPYARALL
                     ERFHEFESGPKGALTRYIVSLDDKS"
BASE COUNT          224 a          158 c          109 g          241 t
ORIGIN      
        1 tgtgatttat catcaagtga cacaatataa cgagttaatg ctcctttggg gccactctca
       61 aactcatgaa accgttcaag taatgctctt gcataaggcg ttgtgtcaaa aatattaaaa
      121 tcataaagat agcgtgcagc gtaaccatac aacccatgta cccaaagccc ttctcttatc
      181 agctctctga gataatatgc aatactgtca agtatcttta ccttccgttg catatcaagc
      241 aaaatactta atgaataaaa tgcataatag aacttgtcaa tgttgttgag tattctgtcc
      301 agctcactct tatcaagctt cttctctctg taatccttct tgtattcatt cttacaggcc
      361 tttatatatt gtttcataag gctcttcctt ttaccgtacc cttttcgaat agttccctgg
      421 tattttccca gctcatacat ttttccgttt tcccgaaaat cttctctgtt aaatcccagt
      481 tcagcaagat actcccggag ataacgttta acggcagctt catcgctttc tgtaccagag
      541 taatccaggc agggacaata tgatttttgc aaaaaattat agatatgata ataaaaccct
      601 gtttcattct ccggacgata tgtgttatat atatccagca caataaaaac aactccaaag
      661 cttttcagaa aatagttacg gatatattcg gactttaatg ttgaatacac agggtaagaa
      721 ttcatcatgt aa
//
LOCUS       DAZGAP010000521          726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGAP010000521 DAZGAP010000000
VERSION     DAZGAP010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.524"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..>726
                     /locus_tag="ACHLAM_005521"
     CDS             13..>726
                     /locus_tag="ACHLAM_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO7856854.1"
                     /translation="MNLRKKHICIAISLVLNPASISQVSAQADTNKLTVEVDEASLGH
                     DVYGDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGGISTKEG
                     LYTGESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNN
                     YSSSEASGDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAVGF
                     NALANASNALAVGSQSSAGG"
BASE COUNT          213 a          139 c          202 g          172 t
ORIGIN      
        1 aagagggtat atatgaacct cagaaaaaaa catatctgta tagccatatc acttgttctt
       61 aatccggcat ccatatcaca ggtcagtgct caggcagata caaataagtt aactgttgaa
      121 gttgatgaag ccagccttgg ccatgatgtc tatggcgatg actggaagag cattgaaata
      181 ggtacaacga aatttacagc aaaagatgaa tcaaagggaa ttaatttcga ccataaatca
      241 ccggtggctg aaggacgtaa ctctatcgcg attggtacca gtgcagaggc tggaggtggc
      301 attagcacga aggaaggttt gtacacaggc gagtctattg cgataggagc gaaaacgaaa
      361 gcaactaaag aacagtcaat tgcaataggt ggcgatacca aagctaccgg atggggggct
      421 attgttattg gtggagatga tttaacccct ttgcatggtg acagattcgg tgataaagaa
      481 attccgaata attactcgtc atctgaagca tcaggagacg gaagtattgt tgtgggctca
      541 aggagcacag ctgcaggaga cctgtctgtg tccctgggct caataagctc tgccaacggg
      601 acggctgcta atgcactggg agccacggcg gaagctaacg gggatatggc gactgcggtt
      661 ggttttaatg cgctggctaa tgccagtaat gctctggctg ttggtagtca gtcctctgcc
      721 ggtgga
//
LOCUS       DAZGAP010000522          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGAP010000522 DAZGAP010000000
VERSION     DAZGAP010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.525"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>715
                     /locus_tag="ACHLAM_005522"
     CDS             <1..>715
                     /locus_tag="ACHLAM_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7856855.1"
                     /translation="SERNILTASLIIKNEGGYAINWQDFDLKIKSAKMVLLCNPHNPT
                     GTVWSEEELLKIATSCTRHNVWLCSDEIHSDFVFNRSFTSALKIKKEKVVVFNSISKT
                     FNVPALTGSYLISTDEDYNHKFRTISRYRDFVNSPSVLNIIATIVAYNECEGWLKSLK
                     AHIASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYSFDEFYSRLIHEGKVGI
                     MAGNVYGTEGEGYLRLNLA"
BASE COUNT          223 a          124 c          157 g          211 t
ORIGIN      
        1 atagcgagcg aaatattctg acagcctctt tgattataaa aaatgagggg ggctacgcta
       61 tcaactggca agattttgat cttaagatta aaagcgcaaa gatggtgtta ttatgtaatc
      121 ctcataatcc gacaggaaca gtctggtcgg aagaagaact gcttaaaatc gctacaagct
      181 gtacaaggca taatgtctgg ttatgttcag atgaaattca tagtgatttt gtttttaatc
      241 gtagctttac atccgcgctt aaaataaaaa aagaaaaagt agtggtattt aactcgatat
      301 cgaaaacatt taatgttcct gcgctaacag gatcgtatct gatttcaact gacgaggatt
      361 ataatcataa gttcagaacg atatcgaggt atcgcgattt cgtaaactcc ccatcagtac
      421 tgaatataat tgcaaccatt gttgcctata atgaatgtga gggatggtta aagtcattaa
      481 aggcacatat cgcctctaac attcaattca cccagcagta tctgaacgaa aatactccag
      541 aattgatcgt aaggccagct gatggttgct atttttcgtg gatagattgc tctgctattg
      601 gttattcctt tgatgaattc tacagtcgat taattcatga aggcaaagtg ggcattatgg
      661 cggggaatgt ctatggaacg gagggggagg gctaccttcg tctgaacctg gcctg
//
LOCUS       DAZGAP010000523          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGAP010000523 DAZGAP010000000
VERSION     DAZGAP010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.526"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..355
                     /locus_tag="ACHLAM_005523"
     CDS             <1..355
                     /locus_tag="ACHLAM_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7856856.1"
                     /translation="AAIVGSFFTGGATMALWGAALSAGGLTATTMLFSLGVSMILGGV
                     AQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDIST
                     RDEGGDGKVVVIGRG"
BASE COUNT          200 a          152 c          199 g          158 t
ORIGIN      
        1 agcagccatc gtgggctctt tcttcaccgg cggcgcaacg atggcgttgt ggggcgcagc
       61 cctgagtgcc ggagggctga ctgccaccac gatgctgttc tcactggggg tcagcatgat
      121 actgggtggc gtggcacaga tgctggcccc gaaggcaaag acgccggagt acaaaagcac
      181 ggataacggt aaacagaaca cgtatttttc gtcactggac aacatgattg cccagggtaa
      241 ccctatgccg gtgccttacg gtgaaatgct ggttggttca cgacggatat cccaggacat
      301 cagcacccgt gatgagggcg gagacgggaa agtggtggtt atcgggcggg gatgaaaata
      361 aaaaaatccc gcagagttag cggagctgcg ggaaataacg atgaagatta actgtaagga
      421 gttattatta tgtcacagca aaaaaacggt aacgcagcga aattataagc gctacatgga
      481 ttttgcgaaa atgtgaataa tttcagaaat tttctgttgc ggggctgaac taaaaaacgc
      541 tcccgcaata cacctggata ttaacgggag gaacgaatag tcaccttaag gagttgcgat
      601 taattgcttt tattgaccgc aggtaatcta cccatccggc aaggtttcct ccacgggaat
      661 ttgccggaaa tgtgaagaat ttcagaaatt ttattccgtc atgacacag
//
LOCUS       DAZGAP010000524          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGAP010000524 DAZGAP010000000
VERSION     DAZGAP010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.527"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..245
                     /locus_tag="ACHLAM_005524"
     CDS             <1..245
                     /locus_tag="ACHLAM_005524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856857.1"
                     /translation="RMHTYEYPTATISCLTKLNNENILNIITGIEVILLAAKKPACKD
                     RTDKIAEMLKIPFSVVKEFIDEQALLNKSNIQGTGS"
     gene            complement(292..444)
                     /locus_tag="ACHLAM_005525"
                     /pseudo
     CDS             complement(292..444)
                     /locus_tag="ACHLAM_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
BASE COUNT          240 a          150 c          138 g          176 t
ORIGIN      
        1 ggagaatgca cacatacgaa tatccaacag caaccatcag ttgtttgaca aaattaaata
       61 atgaaaatat attaaatatt attacaggta tagaagtaat actcctcgca gcaaaaaaac
      121 ctgcatgtaa agacagaaca gataaaattg ctgaaatgct gaaaatacca ttctctgtcg
      181 tcaaagaatt tattgatgaa caggctttat tgaacaaatc aaacattcag ggaactggca
      241 gttgaacaaa acgccagtcc gaagactggc gcagtgataa aacattctgc atcataccac
      301 attctgctcc tgaagatgat gcagacgatg ctcccagttt cccgggtaat acccgggcgt
      361 cagcagtacg cggcgtcgct cattacagag cgagttacca atcactactt ctccggccac
      421 gccacacagc gacagttgta tataggggtt tggggagcaa tgaaacagta aacgccatta
      481 agaggttaat aaaaatagcc aattatttat taatcaataa attatatcta ttcattgaac
      541 gctctcagct cccccttact ggggtccgcc tggaattcag aaaataaacc acgttaagac
      601 tgttttttag ccaaatgact ttcagggatc gcctgaatgg cgggacaaca gcggttttgg
      661 tgtccgaaat cgtacaattc tgtacatcta cacaagggaa ttat
//
LOCUS       DAZGAP010000525          703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZGAP010000525 DAZGAP010000000
VERSION     DAZGAP010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.528"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>703
                     /locus_tag="ACHLAM_005526"
     CDS             <1..>703
                     /locus_tag="ACHLAM_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7856858.1"
                     /translation="TNSLKDNFSFQYLEAERFGPRDNIPLSQHNYHEGWLGKNGEHTI
                     EVLESLVTRRRLNFSAQEDPRKHEGIINNNVFNHIEAWMSEISPGHRISPRIEARANV
                     AFNVIIPSPGQLTKPINIGFGFSYALSIVTALMLASKNELVIIENPEAHLHPRGQSYL
                     GRLIALSAKAGIQVIIETHSDHLLNGIRVIARTDPTFDASLFTLYYISRENDETSVAK
                     IKINNEGKLSEWPRG"
BASE COUNT          232 a          118 c          135 g          218 t
ORIGIN      
        1 taacaaactc acttaaagat aacttttcat tccaatattt ggaagcggag agatttggtc
       61 caagagataa tattccatta tcacagcaca attatcatga aggttggctt gggaaaaatg
      121 gggaacatac aattgaagtt cttgagagtt tggtcacaag gaggcgttta aatttttctg
      181 ctcaagagga tcctaggaaa catgaaggaa ttattaataa taatgttttc aatcatattg
      241 aagcatggat gtctgagatt tcaccagggc atcgtatctc acctagaatt gaggctaggg
      301 cgaatgttgc atttaatgtc ataatccctt cacctggaca attaacgaaa cctattaaca
      361 taggatttgg atttagttat gctcttagta ttgtcactgc tttaatgctg gcatcaaaaa
      421 atgaactcgt tattattgaa aatcctgagg ctcatctaca tccaagaggt caaagctatt
      481 taggacgact tattgcttta tcagcaaaag cagggatcca ggttattatt gagactcata
      541 gtgatcatct tcttaatggc ataagagtaa ttgcacgcac agatccaact tttgatgctt
      601 ctctttttac tctttactat atttcacgag aaaatgatga aacatctgtc gccaaaataa
      661 aaataaataa tgaagggaaa ctttccgaat ggcctagagg gtt
//
LOCUS       DAZGAP010000526          702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGAP010000526 DAZGAP010000000
VERSION     DAZGAP010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.529"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..672
                     /locus_tag="ACHLAM_005527"
     CDS             <1..672
                     /locus_tag="ACHLAM_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856859.1"
                     /translation="PPQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQ
                     SGSRAPETIMQEEMRKYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQA
                     LRFVAAKGHQKAIIVYHDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVD
                     VATGEWATWSPVNYESRVIFPPAGITKMSNKELSNDHVTEVQLFNLKQKTYVSMVKDL
                     VSRFQ"
BASE COUNT          212 a          139 c          169 g          182 t
ORIGIN      
        1 cccccgcaac tgacagacaa agatttattc ggtaatgaaa ccactctggc tgtatccgag
       61 gaggatattc aggctgcgct tgaaggagat gagtttcgtg tgcctctcaa ttctcccgta
      121 atacttgtcc agtctggcag tcgcgcaccg gaaaccatta tgcaggagga gatgagaaaa
      181 tattatactg tttccacatt ctctggtatc ccggacaggc aaaagcctct gacttgtaac
      241 aaaaataaag ataaaaatga gaacgaggat gttgccagtg ctgaaaatat gaactggatg
      301 caggcattgc gttttgtggc tgcgaaaggg catcagaaag cgattattgt ttaccatgat
      361 acgttgcaga caggaaaata tgactctgcg ctgaaatcaa cagtatggtc cgactataaa
      421 aatgaaaaac tcactgacgc tatctccctg cgctacctgg tacgtttcac gctggtggat
      481 gtggcaacag gtgagtgggc tacctggtcg ccggtgaatt atgaatcgag agtaattttt
      541 ccgccggctg gaataacaaa aatgagcaat aaagaactat caaatgacca tgttacggaa
      601 gtgcaactgt ttaatttgaa acaaaaaaca tatgtatcga tggtgaaaga cttggtgagt
      661 cgattccagt aatcgttatt gttatgctgc cgcctgtagt tt
//
LOCUS       DAZGAP010000527          695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGAP010000527 DAZGAP010000000
VERSION     DAZGAP010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.530"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..314
                     /locus_tag="ACHLAM_005528"
     CDS             69..314
                     /locus_tag="ACHLAM_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013184986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7856860.1"
                     /translation="MSLIEDWSSADIKAALEKRDTNLSRLSRENNLQPRTLGNALRVP
                     YPKGEKIIARAIGVEPQVIWPTRYERRARRKYVGCND"
     gene            295..>695
                     /locus_tag="ACHLAM_005529"
     CDS             295..>695
                     /locus_tag="ACHLAM_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7856861.1"
                     /translation="MWVAMTELISFSDLPSSLAGLHKKAKREAWKTRLKPGVKGKVLE
                     CEIGALPLTVQQAVRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQLM
                     VERLNALTENQRQVADARIAIVSEVLKVAQQP"
BASE COUNT          207 a          144 c          187 g          157 t
ORIGIN      
        1 tgagtaactc aatagagtag cacacatgag taataaagtt tgccatagaa agtacacagg
       61 gggccattgt gagtcttatt gaagattggt cttccgccga tataaaggca gccttagaaa
      121 aacgcgatac caatttgtca cgtttatcaa gggaaaataa tttgcagcct aggactttgg
      181 gtaatgcttt gcgagttccc taccctaaag gtgaaaagat aattgctagg gctataggtg
      241 tagagccaca ggttatttgg cctactagat atgaacgacg tgcgcggagg aaatatgtgg
      301 gttgcaatga ctgaattgat ttcattcagt gacctaccta gttctttggc aggtctgcac
      361 aaaaaagcaa agagggaagc gtggaaaacg cggctaaaac caggggtaaa agggaaagtt
      421 ttagagtgtg aaataggagc tttgccttta actgttcagc aggcagtacg cgaacgctat
      481 gccctgcaac tgatgacgca aaaagccgat gaatcaccgg ctccggtagt gacaaaggcc
      541 aggcgctcac ctgccgtggt tgatgcggtg gaggcatatc gcggatcacc acaactgatg
      601 gtcgaacgcc ttaatgccct gactgaaaac cagcgccagg tggctgatgc acgaatcgcg
      661 atcgtcagcg aagttctgaa agtcgcgcaa caacc
//
LOCUS       DAZGAP010000528          679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGAP010000528 DAZGAP010000000
VERSION     DAZGAP010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.531"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>679
                     /locus_tag="ACHLAM_005530"
     CDS             <1..>679
                     /locus_tag="ACHLAM_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFO7856862.1"
                     /translation="ETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQRIINPQFSFN
                     LSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLNEILQLKES
                     SVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYSESMEDAQL
                     FCAYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHNSSSSVLMAGF
                     FREGELKL"
BASE COUNT          214 a          115 c          130 g          220 t
ORIGIN      
        1 ggagacttta tcttgttggt tcgaaaataa aaggattatc gcattttgtg atgaaactca
       61 aacattttcc tttgaaaaag gaacaaatta cgctgatctt cagcgaataa taaatcccca
      121 attttctttt aacttatctg ttgtgttaag atgtcctaga gccgttactg attatttaat
      181 agctgtcagc cctgcctcat accaaattac ttcccctagg aataacgaac cggatacgct
      241 aaaagaatta gttgttacag atccactctc cacattgctt aatgaaattt tacaattgaa
      301 agaaagttct gtactgcctg atgagattac tgtccttgtt cctacggatt tagtgaaaaa
      361 ttccctccag aatgatatga gaactttggg ggtgaatatt gaaacagtat ctcgttttcg
      421 aggtatggaa tcatctgtcg tgattacatt gtattccgaa agcatggagg atgcacagtt
      481 attttgtgct tattcaagag ctacaactgc ttttattgca atttatgatt cggagaaatt
      541 agcatggaga gaaaaaaata aatttttgca taaactggtt aacagaaagg aggttcagag
      601 agcaattaat gaagcaaagc ataattcttc ctccagtgtt cttatggccg gttttttccg
      661 cgaaggagag ttaaaactt
//
LOCUS       DAZGAP010000529          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGAP010000529 DAZGAP010000000
VERSION     DAZGAP010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.532"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..280)
                     /locus_tag="ACHLAM_005531"
     CDS             complement(<1..280)
                     /locus_tag="ACHLAM_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856863.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRV"
     gene            complement(363..>673)
                     /locus_tag="ACHLAM_005532"
     CDS             complement(363..>673)
                     /locus_tag="ACHLAM_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856864.1"
                     /translation="RNNLSYYSRWYTNPAGNLSVRVCMFRRGGVWTPCLYYRQGMYRN
                     EYTGWYQPLPREVIYLNRFTGRISRRRTWHAACRMAEQLTTLRYRFGLQGDGIVAQPC
                     "
BASE COUNT          183 a          188 c          163 g          139 t
ORIGIN      
        1 tcacacgtat atgccacaga aacggccgga aaagtcctga tttcagttgg tcaccaccgc
       61 ttttgtagcc cagatgacgt gcaatacttt cagtatattc aaatgcatca ttctgcattc
      121 cagccgcctt agccagcgag ctgtacgcat cacgaagtat cgtcatacct tccagttgat
      181 actcacacca cgcacggaca gccgacactg gtgcgattac ccgaagaatg gtgcaggcac
      241 gccatacaac aacgtaaagg agtattaaca gactatgcat gtgttcacct cagatacaaa
      301 aaataacggg aacacacctg ccctgcggga agcgtgttcc cgacagggta ataaatgata
      361 aatcagcagg gctgtgcgac gattccatca ccctgcagac caaaccggta acgcagggtg
      421 gtcagttgct ccgccatacg gcaggcagca tgccatgtcc tgcgacggga tatccgtccg
      481 gtaaaccggt tcagataaat gacctcacgc ggtaacggct gataccatcc tgtgtactcg
      541 ttgcgataca ttccctgccg gtaatacagg cagggtgtcc agacaccgcc acgacggaac
      601 atacagaccc gcacgctcag attgccggcc ggattagtgt accagcgact gtaatatgac
      661 aggttattgc ggg
//
LOCUS       DAZGAP010000530          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGAP010000530 DAZGAP010000000
VERSION     DAZGAP010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.533"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..602)
                     /locus_tag="ACHLAM_005533"
     CDS             complement(<1..602)
                     /locus_tag="ACHLAM_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7856865.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          167 a          168 c          159 g          179 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata
       61 gggatctcac gcaactccac atcccagtag cgggcgaatt tatgccagca gatttgtacc
      121 ggaccgcaca ccaggtttgg tttatccgtt ggtttgcctg cggcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggtgca ggcgcatgcc acagatcggc aaccatgttt
      301 acgcaacgca ggtcgattgc agcggattgc ggatattctt ctttgtcgat ccagttttta
      361 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctgaaatgca
      481 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      541 tttgcaccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      601 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      661 agcaaggcat tac
//
LOCUS       DAZGAP010000531          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGAP010000531 DAZGAP010000000
VERSION     DAZGAP010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.534"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..198
                     /locus_tag="ACHLAM_005534"
     CDS             <1..198
                     /locus_tag="ACHLAM_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7856866.1"
                     /translation="WGISGEAELAVMQAIAEKPGALRVLTHTLNQAWLTASGEGAALT
                     EKHINAAFKEVYTNPELLSQV"
     gene            201..422
                     /locus_tag="ACHLAM_005535"
     CDS             201..422
                     /locus_tag="ACHLAM_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856867.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKAVSMSDSEECGDLLFEFNDRTR
                     MAISAGLDREGATEVYSGICRSVGAKQVS"
     gene            435..>670
                     /locus_tag="ACHLAM_005536"
     CDS             435..>670
                     /locus_tag="ACHLAM_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856868.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TAPVGNVQGTGEIEITIQTNGLRRRVKATRISGCT"
BASE COUNT          175 a          160 c          181 g          154 t
ORIGIN      
        1 tgggggatca gtggtgaggc cgagctggcc gtcatgcagg ctatcgctga aaagccggga
       61 gcgttacgcg ttctgacaca tacgcttaac caggcgtggc tcaccgccag cggtgaaggc
      121 gcggcgctga cagaaaaaca tattaatgcg gcctttaaag aggtttatac caaccctgaa
      181 ttactctcac aggtgtgatt atggcggtat ttaatattcc tgatatttac ggacgctttt
      241 acctggttaa tttcgataac gtcaaagcag tctccatgtc tgacagtgaa gaatgtggcg
      301 atttactctt tgaatttaat gatcgcaccc gaatggcaat atcggcagga cttgatcgtg
      361 aaggtgcgac agaagtttac agcggaatat gccgttctgt tggtgcgaaa caagtcagct
      421 aaatgaggtg ttatatgaat atgcaatcct gcggtaacaa aatgaattta ttcgactccc
      481 tgaacagcgc gcgccgtctg accgaacttg ccggtgcggt actggaacgc agtaagcgct
      541 atccgcaacg ttttgcactg aaaaccacgg cgccggtagg caacgtgcag ggaaccggtg
      601 aaattgaaat caccatacag accaacggcc tgcgccgccg tgtgaaggcc acccgcatca
      661 gcggctgcac
//
LOCUS       DAZGAP010000532          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGAP010000532 DAZGAP010000000
VERSION     DAZGAP010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.535"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..198
                     /locus_tag="ACHLAM_005537"
     CDS             <1..198
                     /locus_tag="ACHLAM_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7856869.1"
                     /translation="WGISGEAELAVMQAIAEKPGALRVLTHTLNQAWLTASGEGAALT
                     EKHINAAFKEVYTNPELLSQV"
     gene            201..422
                     /locus_tag="ACHLAM_005538"
     CDS             201..422
                     /locus_tag="ACHLAM_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856870.1"
                     /translation="MAVFYIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            435..>670
                     /locus_tag="ACHLAM_005539"
     CDS             435..>670
                     /locus_tag="ACHLAM_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856871.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCT"
BASE COUNT          176 a          158 c          179 g          157 t
ORIGIN      
        1 tggggtatca gtggtgaggc cgagctggcc gtcatgcagg ctatcgctga aaagccggga
       61 gcgttacgcg ttctgacaca tacgcttaac caggcgtggc tcaccgccag cggtgaaggt
      121 gcggcgctga cagaaaaaca tattaatgcg gcctttaaag aggtttatac caaccctgaa
      181 ttactctcac aggtgtgatt atggcggtat tttatattcc tgatatttac ggacgctttt
      241 acctggttaa tttcgataac gtgaaggtga tttcactggc cgaaaataaa gaatgtggcg
      301 atttactttt tgaatttaat gaccgcacac gaatggtgat atctgccgga cttgatcgcg
      361 aaggtgcgac agaagtttac agcggaatat gccgttctgt tggtgcgaaa caagtcagct
      421 aaatgaggtg ttatatgaat atgcaatcct gcggtaacaa aatgaattta ttcgactccc
      481 tgaacagcgc gcgccgtctg accgaacttg ccggtgcggt actggaacgc agtaagcgct
      541 acccacaacg ttttgcactg aaaaccacgc cgccggtagg caacgtgcag ggaaccggtg
      601 aaattgaaat caccatacag accaacggcc tgcgccgccg tgtgaaggcc acccgcatca
      661 gcggctgcac
//
LOCUS       DAZGAP010000533          669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZGAP010000533 DAZGAP010000000
VERSION     DAZGAP010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.536"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            85..>669
                     /locus_tag="ACHLAM_005540"
     CDS             85..>669
                     /locus_tag="ACHLAM_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HFO7856872.1"
                     /translation="MELFAITDNTVGTRIVRIVTDRPTQNVVTQLFSEQKTFFEERYT
                     EGVEFSGGYITSGDEFFVIPDFDDVIAVLDAINNPTSIPPWEPEEISAFNIIALFSGY
                     PEEDGKPVTALIQSFDKRQVIDNRRTILQKMFQASNTFCQSTEHGIVIDNKLSAILAG
                     TELKFKSFHMLRRIFDVDAYFREATNEELTSFSSH"
BASE COUNT          229 a          113 c          123 g          204 t
ORIGIN      
        1 aaaaccactg ctaaaagcat caggtaatcg tgttaatttg attaagctta ctgattacac
       61 atatatagta atacaggagt aagtatggaa ttatttgcaa taacagataa tacagttggc
      121 acacgtattg tcagaattgt tactgacaga ccgactcaaa atgtagtcac acagttattc
      181 agtgaacaaa aaacattttt tgaagaacgc tacacagaag gtgtggaatt ttcaggtgga
      241 tacatcacta gtggagatga gttttttgtc attcctgact ttgatgatgt tatcgctgta
      301 ttagatgcca ttaataatcc cacatctatc cctccatggg agccggaaga aatatcagcc
      361 ttcaatataa tcgctctttt ttcaggctat cctgaagagg atggaaaacc tgtaactgca
      421 ttaattcaaa gcttcgataa aagacaagtt attgataaca gaaggacaat tttacaaaaa
      481 atgtttcagg caagcaatac tttttgccag tctactgagc atggtatagt cattgacaat
      541 aagctaagcg ctattctcgc aggtacagaa ttaaaattta aaagtttcca tatgctccgc
      601 agaatatttg atgtagatgc ttatttcaga gaggcgacta atgaagaatt aacaagtttt
      661 tcttctcat
//
LOCUS       DAZGAP010000534          668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGAP010000534 DAZGAP010000000
VERSION     DAZGAP010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.537"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            194..>668
                     /locus_tag="ACHLAM_005541"
     CDS             194..>668
                     /locus_tag="ACHLAM_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856873.1"
                     /translation="MGCNKKSNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIYFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGTPVGLPFEIDTYKNSSMIAITGQSIS"
BASE COUNT          222 a          106 c          140 g          200 t
ORIGIN      
        1 ataatattaa tctcacaaaa tgacattatc tgaggtaatt gatgccccaa gagaacgaaa
       61 aatggtaaaa agaaatacag gttagtacct agggtggcag aagttgaggg ggagtcgtac
      121 tattctggtg atggattgaa taaagctcac ctattaccag atacgatgat taaagctctt
      181 gaggattcta aaaatgggat gtaataaaaa atcaaacaaa tttttgcttt ttgtgtcatg
      241 catgctcttg tcgtttgtaa cgtactcaca agacagttct aatcctcagt tttctgacta
      301 cctggttcct gtaagcaacg gaccatttga aaagaatatt tactttaata aagagcagga
      361 aaattactcg caacactgga agaatgcagt ccaagaggaa ctaaaaaagc ctgttaattt
      421 tgcagggcac tttagaatct atacagcctt tggcggtcat ggtaaagaat gccttcgtga
      481 taattgggta tgtggttggg ttattgataa actatcaggg gaagttgtag caacacttcc
      541 atcagataat aatggaagtt ataactatgc tgatgtgtct gataatggta cccctgttgg
      601 tcttccattt gagattgata cttataaaaa tagttcaatg attgcaataa ccggtcaatc
      661 tatctcag
//
LOCUS       DAZGAP010000535          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGAP010000535 DAZGAP010000000
VERSION     DAZGAP010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.538"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>667
                     /locus_tag="ACHLAM_005542"
     CDS             <1..>667
                     /locus_tag="ACHLAM_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO7856874.1"
                     /translation="NEILHDKELLLSLENPRRFIFSKWTLREGWDNPNVFQICKLRSS
                     GSTTSKLQEVGRGLRLPVNEYMCRVKNRDFTLNYYVDFTEKDFVDSLVKEVNDSSFKE
                     TVPGKFTQELKDKILSQYPELSSRTLMNEIFDAEIIDDNENFKDSDAYSRLKAKYPAA
                     FPAGVKPSKIKKATDGKKRTKMRIGKFSELKELWDLINQKVVIEYKINSENEFLSIFK
                     SFMI"
BASE COUNT          223 a          112 c          130 g          202 t
ORIGIN      
        1 caacgaaatc cttcatgata aagagcttct attgtcttta gaaaatcctc gccgtttcat
       61 tttctctaaa tggacgttgc gtgagggatg ggataaccca aatgtctttc agatttgtaa
      121 gctccgttcg agcggtagca ccacatctaa acttcaggag gtagggcgtg gtttacgtct
      181 tccagtgaat gaatacatgt gccgtgtgaa aaaccgcgat tttactctca actattatgt
      241 tgattttacg gaaaaagatt ttgttgattc tcttgtaaaa gaggttaatg atagttcttt
      301 caaagaaact gttccaggta agtttactca agaacttaag gataaaatct tatctcagta
      361 tcctgagctt tcatccagaa cactcatgaa tgagatcttt gatgctgaaa ttattgatga
      421 taatgaaaac ttcaaagatt ccgatgccta tagccgttta aaagcaaaat atccggctgc
      481 attccctgcc ggggtgaaac caagcaaaat aaaaaaagcc acggatggaa aaaagcgtac
      541 taaaatgcgt attggtaagt tcagtgagtt gaaagaacta tgggatttga tcaatcaaaa
      601 agtagtaatt gagtataaaa taaatagtga aaatgaattc ttatccattt tcaaatcatt
      661 catgatt
//
LOCUS       DAZGAP010000536          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZGAP010000536 DAZGAP010000000
VERSION     DAZGAP010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.539"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>665
                     /locus_tag="ACHLAM_005543"
     CDS             <1..>665
                     /locus_tag="ACHLAM_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856875.1"
                     /translation="GIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVI
                     AYRICRDYLTIAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYES
                     EAENQWVVQDTLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWNPADSNLNATGS
                     NKRKYGLVTLKGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAGPGWWRHLLPPAKEAM
                     VFD"
BASE COUNT          180 a          153 c          177 g          155 t
ORIGIN      
        1 gggatccata tcaccgaagc cacgacacca tggtgtgcgc ttagcggtaa gtctccactt
       61 gaagcgatag attttccggt tccttttaaa ggtgagcagg caatccgatg catggctgac
      121 ggagaagtga ttgcttaccg catctgcagg gattatctga cgattgcatg ggaatccggt
      181 ccgctgaatt tttccggctc atttgttctg gtgaaacact atatccagcc gggtgagaaa
      241 gaaaccagtg gactgcattt ctatactctg tatatgcatc ttgcgccgta ttcagcttat
      301 gaatctgaag cagaaaatca gtgggtcgtt caggacactc taaaagcata ttctgaaatg
      361 gactggctga cggcgaaact gacttcagat gaaacttctc cacaaattgc agggtacatg
      421 cctgaagggg ccagagtgga gtggaacccg gcagacagca atcttaacgc tacaggaagt
      481 aacaagagaa aatacggtct tgtgacgcta aagggtttac cagaaaaaaa cagcagtact
      541 ctcactcccg gtcagcgcta ctgggttgtt gtggataaca acaatgttaa gccagcgccg
      601 ggggccgggc ccggctggtg gcgacacttg ctgcctcccg caaaggaagc catggtattc
      661 gataa
//
LOCUS       DAZGAP010000537          664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZGAP010000537 DAZGAP010000000
VERSION     DAZGAP010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.540"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..614
                     /locus_tag="ACHLAM_005544"
     CDS             87..614
                     /locus_tag="ACHLAM_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7856876.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     KTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPLQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT          154 a          184 c          213 g          113 t
ORIGIN      
        1 aggagctttc gctcctctga aggggcacat gaacgccccg tctccggtat ccggtttatc
       61 tcacatcttt cagtcaggag gttctcatga gtgcacgtgg tatcaacaag gtcatcctcg
      121 tcgggcgtct gggcaatgat ccggaggtcc gttacatccc caacgggggc gcagtggcaa
      181 acctgcaggt ggccacgtca gaaagctggc gcgacaaaaa gacgggggag atgcgggagc
      241 agacggaatg gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggct ggtgaatatc
      301 tgcgcaaggg cgcgcaggtc tatatcgagg ggcaactccg aacccgtagc tgggaagata
      361 acggtatcac ccgttacgtc actgaaattc ttgttaagac cacgggcacc atgcagatgc
      421 tggggagtgc accactgcag aacgctcagg cacaaccgca gcctcagcag aatggtcagc
      481 cacagagtgc tgacgcgacg aaaaaaggtg gcgcgaaaac gaaaggccgt ggacgtaagg
      541 ccgcgcagcc cgagcctcag ccgcaaccgc cggaggggga ggattacggg ttttcagacg
      601 atatcccgtt ctgactggcc tgactgtgat aaccgccccg ccctgcgcgg ggcatcaccg
      661 gaga
//
LOCUS       DAZGAP010000538          664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZGAP010000538 DAZGAP010000000
VERSION     DAZGAP010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.541"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>664
                     /locus_tag="ACHLAM_005545"
     CDS             <1..>664
                     /locus_tag="ACHLAM_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO7856877.1"
                     /translation="SCLSPTLVCAEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSNGI
                     GLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQYW
                     STYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQKT
                     NFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMGSWIPYMSIADYKGVDKTSSNRQ
                     IR"
BASE COUNT          204 a          130 c          144 g          186 t
ORIGIN      
        1 tatcctgcct gtcacctaca ctagtctgtg cagaacagcc gcatggattt attgattatc
       61 gccatgagta tcttgatgat actcgcaccc acgcggaccg cgttgaattt ggtacatttt
      121 ttagtaatgg tatcggtttg atgggagaat tacgctataa caccgatgaa ggtgataaag
      181 ataagtggga cccctcacaa tttggcaata acggaacagg attatctgtt gtttaccgct
      241 ttaagccgct ggatgacaaa aaattctggt tagaacccat gttctggctt gataccacgc
      301 aatattggtc aacttacgaa tatggtcttt ctgcgggtta tgacttcagc aaagaatgga
      361 aagtttcagg tcggtttcgt tatgacatgg ataaagccac cgataaatcc aaaggttatg
      421 gcaacgatga tcgtaataac cgccgttacg atgtgtggat cgactatcga ccacaaaaaa
      481 ccaacttcca atatcagtta aacctggtct attacaataa tggttattta acctggaatg
      541 atggccataa aaactatacc gcatcattta aagtagggta caaaatggga tcgtggatac
      601 cctatatgag tattgcggat tataaaggtg tggataaaac atcaagtaat cgccaaatcc
      661 gctg
//
LOCUS       DAZGAP010000539          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZGAP010000539 DAZGAP010000000
VERSION     DAZGAP010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.542"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..539)
                     /gene="tnpA"
                     /locus_tag="ACHLAM_005546"
     CDS             complement(81..539)
                     /gene="tnpA"
                     /locus_tag="ACHLAM_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO7856878.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          156 a          185 c          121 g          201 t
ORIGIN      
        1 ttttcaaatg cgcctataag gctctcttac cagccgcgcc ctaacaggcg cacacgatct
       61 gacatttgca tccaacttcg ttacttacgg cccgtaaacg ggctgcccgg atagggaatc
      121 gataactgct ctcccatttt atcctcttca agctggtgct ttatgtaatc ctgtatcttc
      181 gccgtgttct tacccaccgt atcgacgtag taccctctgc accagaactc tctgttcctg
      241 tatttgaatt tcaaatcacc aaactgctcg taaagcatca gactgctttt ccctttcaga
      301 tatcccataa agccggatac actcattttg ggcgggatct ccacaagcat atggatatga
      361 tctgcacagc attcagcttc aagaatccgt acgcttttcc actcacacag ctttctcaaa
      421 atactgccta ttgctctacg cttctctctg tagaacactt gtcttcggta ttttggcgca
      481 aaaactatgt gatatttaca gttccatcgg gtgtgcgcta agctcttttc gttccccatt
      541 gggaccccct tttgatttct tgtttgacac ttgcagttgc cagaccgcaa ggtgttttaa
      601 caaatcaaaa ggggttttaa taactggctc aaagctgaaa gctttccgga acccccagcc
      661 tag
//
LOCUS       DAZGAP010000540          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZGAP010000540 DAZGAP010000000
VERSION     DAZGAP010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.543"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>661
                     /locus_tag="ACHLAM_005547"
     CDS             <1..>661
                     /locus_tag="ACHLAM_005547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856879.1"
                     /translation="TLAVMENENGHFIALEGNRRVCALTLLNDPDLCPENERAYFRKL
                     SDNSDVIPDAVECVLFENREDANKWIELRHNGEQDGAGLLTWNATQKARHFGSSDNTL
                     ALKLLDYAETKGFISKQKRDGILTTASRFLGNPVFRSTMGIRTGRSESNVMIDVSRDE
                     FDKVLERFINDLINGENNVTSRSKKEDWENYAKQLRSEGIAPKGHCEPFALFSTEHSN
                     AS"
BASE COUNT          225 a           98 c          148 g          190 t
ORIGIN      
        1 aacacttgcg gtgatggaga acgaaaatgg acactttatt gcccttgagg ggaataggag
       61 agtttgtgca ttaactctgc ttaatgatcc tgatttatgt ccagaaaatg agagggctta
      121 tttcagaaaa ctatctgata atagtgacgt cattcctgat gcagttgaat gcgttttatt
      181 tgaaaataga gaggatgcaa ataaatggat tgaattgcgc cataatggag aacaagacgg
      241 tgcggggctt cttacttgga atgcaactca aaaagcaaga catttcggtt cttctgataa
      301 tacattagcg ttaaaattat tagattatgc tgaaactaaa ggatttattt cgaagcaaaa
      361 acgagatggc attttgacaa cagcatcgag attcttaggt aatcctgtat tcaggtcaac
      421 tatgggaatt agaacagggc gttcagaatc taatgtgatg attgatgttt ccagagatga
      481 atttgataaa gttcttgaaa ggttcatcaa tgacttaatt aatggtgaaa ataatgtcac
      541 atcaagatca aagaaagaag actgggagaa ttatgcaaaa cagctaagat cggaaggtat
      601 tgcccccaaa ggacactgtg aaccatttgc tctttttagt acggaacata gcaatgcttc
      661 t
//
LOCUS       DAZGAP010000541          654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZGAP010000541 DAZGAP010000000
VERSION     DAZGAP010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.544"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            8..208
                     /locus_tag="ACHLAM_005548"
                     /pseudo
     CDS             8..208
                     /locus_tag="ACHLAM_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
BASE COUNT          204 a          148 c          122 g          180 t
ORIGIN      
        1 tattgtcatg acaaataaac acctatcact gttttctcct tttatgctga cagaaaaaat
       61 aaagccatgt aatcgtatcg ttatggctcc tatgacgacc tggtcagcta atcctgatgg
      121 aaccatctct gagcaagaac tcgaatatta taaacgtcgc tcacaaaata ttggtttagt
      181 aattacagga tatacctatg tcacgccaag gaaattcaga catcgaaagg aactggctgg
      241 tttccgttaa tggattaaat atcgattact ctcccgagag taaaactatc aaatcaatga
      301 gtaaccgata cagcctcgca aattctttca gataattaac ccgtcactgc ggcacgttct
      361 ctcgtaaggc gtgcctgcag tgtaatccag tatgtatccg gcatagttga cggtttaata
      421 gactcaattc ctctccctgg ttacctacaa cgatatcttg tataacaccc caggagatac
      481 gacgttacga tgagatgcaa tatgctcctc agcgccaatc aacgccagga ctgggccagt
      541 cttgagatca gactcattcc cgtaactgat ttcaggcgac atatatcaat gacgttgggg
      601 caataaaaga tagccgatgt aatacatcta ttcaaatttg tgatacatgt caaa
//
LOCUS       DAZGAP010000542          649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZGAP010000542 DAZGAP010000000
VERSION     DAZGAP010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.545"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..558)
                     /locus_tag="ACHLAM_005549"
     CDS             complement(<1..558)
                     /locus_tag="ACHLAM_005549"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856880.1"
                     /translation="MGSDIPVFYSWTGPFMDSVVSFISAYFPIFLVGAVFGTLMNASG
                     AALTIAHFISDKMGEKRAIIAVIIASFLLTYGGISVFVVVFAVLPIARTIFIKSNYPQ
                     RLLPSAICAGCLPAYVAPGSAQFINAIPIPVFNTTIYSGLVIGIVGTAVWLLFAVWYM
                     SAKIKNAKIKGEGYGTEVSNDCNLET"
BASE COUNT          245 a          128 c          100 g          176 t
ORIGIN      
        1 agtctcaaga ttacagtcat tacttacttc tgtaccatat ccttctcctt ttatcttagc
       61 atttttaatt tttgcagaca tataccatac agcgaacaat aaccatacag cagtgccaac
      121 gataccaatt actaaacctg aatatatggt tgtattgaac acaggaattg gtattgcatt
      181 tataaactgg gcagaaccag gagctacata tgctggtaag caaccagcac agatagcact
      241 gggtaataat ctttgagggt aattactttt tataaatata gttctcgcaa taggtaaaac
      301 tgcaaaaacc acaacaaaaa ctgatattcc tccataagtt aataaaaatg atgcaataat
      361 aactgcaata atagccctct tctcacccat tttatctgat ataaaatggg caatagttaa
      421 tgctgctcca cttgcattca ttaatgttcc aaaaacagca ccaacaagaa atatagggaa
      481 gtatgcggat ataaaactca caaccgagtc cataaatgga ccggtccatg aataaaagac
      541 aggaatatca cttcccataa agacagccag cattgccatc aaaggggata ctatcagaac
      601 tggaaaacca cgatatatga gatatattag taaaataagt gaaattatc
//
LOCUS       DAZGAP010000543          649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZGAP010000543 DAZGAP010000000
VERSION     DAZGAP010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.546"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..110
                     /locus_tag="ACHLAM_005550"
     CDS             15..110
                     /locus_tag="ACHLAM_005550"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO7856881.1"
                     /translation="MEGKQPKTLGGNFTILIAKKDGQVLNVIHTK"
BASE COUNT          212 a          121 c          139 g          177 t
ORIGIN      
        1 ataattgttg gaaaatcgag ggaaaacaac cgaagacgtt aggaggaaat tttacaatac
       61 taatagcgaa aaaagatggg caagtgttga atgtaataca cacaaaataa tccactgtac
      121 agtcgattca aatattttac caaggtgtgt tttgtaacaa ttttgtgtac aatatgactc
      181 tacatatcga cgaaaaaacc acttgatttg acaggaacac attcaaaaaa atttctttat
      241 tcatttcagg agcgattttc atctgttact tgaagtatct gtgagattaa gtgactcccc
      301 ctaacgtcgt actaaatagt aattactgtc cataccggga gcagtattcg gcgctcccca
      361 catgcagcag aataaccgca tcagcgaaga tgtattgtat ttttactatc acaatgtgta
      421 cggacggttt atggaaaaga atgaaaatta gatacgcgat ggggcggcag gttacacggt
      481 tatcactacg atagccaata ctggctggtg cattaccgcc gtgaacaata ggacgccgta
      541 ctgctggaaa tttcttacct ttatgactcc ccgcgccgcc gtattgggaa acgggaagca
      601 atcaagatga ggctgatata atgtgcggaa tgcctgagta tttttagaa
//
LOCUS       DAZGAP010000544          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZGAP010000544 DAZGAP010000000
VERSION     DAZGAP010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.547"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..248
                     /locus_tag="ACHLAM_005551"
     CDS             <1..248
                     /locus_tag="ACHLAM_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856882.1"
                     /translation="GLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKE
                     GRVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(268..615)
                     /locus_tag="ACHLAM_005552"
     CDS             complement(268..615)
                     /locus_tag="ACHLAM_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO7856883.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLLPLPF
                     MPFWQVTVLMGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT          159 a          183 c          175 g          123 t
ORIGIN      
        1 caggtctgcc cgcagacggc acttctgcca ctgatctggc ggtggagctg aacggcataa
       61 cgtatcaggc ctgccgtggg gattttgtgg tgcgtctcga cggcagcacc tgtctgcagt
      121 tatggaataa agaaggcagg gtggtacgcc gggagggcga tccgctggaa gtggcgcagt
      181 ggctgcaggc ctgtcatgac gcaggaatag aagttcgcgt acagataaat gaaagtgccg
      241 ctccgtaaga acacgcctgt gcacggttta cacatcatcc catggtggca gccggttaac
      301 tttcctgcgc caccagtgga aagaggccat gcacagtccg aacaaaaaac cccccgtgat
      361 acttataata atggcctcac ctgccaccat tcctgacggc ccccagtaga taaaccacat
      421 tgcacatccc caggaaatgc cccataagcc ccccatcagc accgtgacct gccagaacgg
      481 cataaagggt aatggcagaa gccggatacc cagccgccag agaatacgca gaagaggagg
      541 ggcataattg ctccgccaca tctttttgct gtccatcagg gcaatggccc gggctttttt
      601 ctgctcaaag gtcacaggag cacctccgtt ccatgatgta
//
LOCUS       DAZGAP010000545          639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZGAP010000545 DAZGAP010000000
VERSION     DAZGAP010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.548"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..126
                     /locus_tag="ACHLAM_005553"
     CDS             <1..126
                     /locus_tag="ACHLAM_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856884.1"
                     /translation="PVKWFCVDDVIYLQCGVQPEKQRMTEILSDEFYAAEKRVRG"
     gene            130..>639
                     /locus_tag="ACHLAM_005554"
     CDS             130..>639
                     /locus_tag="ACHLAM_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO7856885.1"
                     /translation="MMILQPMGRKGRAPAHVRPWTPEEDALLIALYPSTPVKNIAVRV
                     KRSFWGVHHRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRISDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHR"
BASE COUNT          182 a          129 c          159 g          169 t
ORIGIN      
        1 cccgtgaagt ggttctgcgt tgatgatgtg atttaccttc agtgtggcgt acaacctgaa
       61 aaacagagaa tgaccgaaat tctgtctgat gagttttatg ctgctgaaaa gcgagtcagg
      121 gggtgatgta tgatgatttt acaacccatg gggcgaaaag gtcgtgcacc cgctcatgtc
      181 cgtccgtgga cacctgaaga agatgcgctg ctgattgcgc tttatccatc caccccggtt
      241 aagaatattg ctgtcagagt aaaaagaagt ttctggggtg tacatcaccg gattgtttta
      301 ttacgcggca cttacccgga gttgctcaaa tgcaaacgtc caagatttaa acatgatgaa
      361 gataaattta tccgaaaaaa tgccagaacg atgaccggta agcaaatagg agaatatctg
      421 gggcgagacc gggattctgt ccacaatcgg gcgcgatata ttggcgtaag catgaaaaaa
      481 tacggagaat tgctgccctt cacccgtata tctgatgatg atgttcgtct tattcgtgaa
      541 ttacgggatg ctgaatcacc acgacgtctt accttccggg aaataggcga gaaatttgaa
      601 ttatccgaga gtacggtaaa ttttatttat caccatcgc
//
LOCUS       DAZGAP010000546          639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZGAP010000546 DAZGAP010000000
VERSION     DAZGAP010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.549"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..625
                     /gene="pagP"
                     /locus_tag="ACHLAM_005555"
     CDS             65..625
                     /gene="pagP"
                     /locus_tag="ACHLAM_005555"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO7856886.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYDP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          169 a          123 c          156 g          191 t
ORIGIN      
        1 gttatgttaa ttgtagcttt gctatgctag tagtagattt ttgataaatg ttttatggtc
       61 acaaatgaac gtgagtaaat atgtcgctat cttttccttt gtttttattc agttaatcag
      121 cgttggtaaa gtttttgcta acgcagatga gtggatgaca acgtttagag aaaatattgc
      181 acaaacctgg caacagcctg aacattatga tttatatatt cctgccatca cctggcatgc
      241 acgtttcgct tacgacaaag aaaaaaccga tcgctataac gagcgaccgt ggggtggcgg
      301 ttttggtcag tcgcgttggg atgaaaaagg aaactggcat ggcctgtatg ccatggcatt
      361 taaggactcg tggaacaaat gggaaccgat tgccggatac ggatgggaaa gtacctggcg
      421 accgctggcg gatgaaaatt ttcatttagg actgggattc accgctggcg taacggcacg
      481 cgataactgg aattacatcc ctctcccggt tctactgcca ttggcctctg tgggttatga
      541 tccagtgact tttcagatga cctacattcc gggtacctat aacaatggca atgtgtactt
      601 tgcctggatg cgctttcagt tttgagacaa atgaaattt
//
LOCUS       DAZGAP010000547          629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZGAP010000547 DAZGAP010000000
VERSION     DAZGAP010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.550"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..597)
                     /locus_tag="ACHLAM_005556"
     CDS             complement(<1..597)
                     /locus_tag="ACHLAM_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7856887.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYL"
BASE COUNT          206 a          122 c          146 g          155 t
ORIGIN      
        1 caaatatcga gttgcagaaa gtcggcaaat aattaacact ttgtctcctt tagtgaaaat
       61 ttttccttct tcaggctcca gtagtaaata atgtatttgg ccaaattgat cggtgagttt
      121 tccttcgcag ggattacctg aagtggcttg atgaccggta attaacgcca tgctaccaat
      181 atattcttct tctgtaatag ctgagctgtg atcgcgtgga atccagggcg caactatttt
      241 accggtataa tgcaccgcaa taatcgtaaa cagtaaactg acaggaacca caaacaggtt
      301 cgagagtggt gattgccaga ccatgaggca ggtatgctgc aacaggatgc caataagacc
      361 aaaaaaacca gccagtaagc agagaacgac gagagctggc aatcttccga tattgagata
      421 atgaagtgcc tggctaatat gacctgtggt tatggaatca taatggtcaa gatgtgcatc
      481 aagagcaccg gagagcatgt gaccgcagat aagcgcaaaa atttctaaaa ggccgatcaa
      541 cagtacaaag gaaatagcga aaagataagg ggtgttatag tcggcgaata aaatcatatg
      601 tcactccgat gtccatttag tgacgcaat
//
LOCUS       DAZGAP010000548          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZGAP010000548 DAZGAP010000000
VERSION     DAZGAP010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.551"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..445)
                     /locus_tag="ACHLAM_005557"
     CDS             complement(<1..445)
                     /locus_tag="ACHLAM_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO7856888.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQMGNVKALFESKPFITLSEPACGAGSMILAM"
BASE COUNT          168 a          175 c          139 g          146 t
ORIGIN      
        1 ccattgccag aatcatactg ccggcaccac aggcaggttc actgagcgtg ataaagggtt
       61 tactctcaaa caacgccttc acattcccca tctgcatctg tgccatcatg ctggccacac
      121 tccatggcgt gaaaaactga ctacggtatt tatcccccag ttccagttgc atgaacacac
      181 tgcccagaaa atcacagaat ccctgctcca gtccctcaat gacatgagca agcaaatgcg
      241 caatgtggat aacatcctgc ttttcataac cactgacgat ccgcagatac ttctgttccc
      301 gggcctcact gaactgaagc cggttttcca gggcaatcac actacagctg acaaagtctt
      361 caaacacctt atgacgtttg tgataacagg ctgtccggtt aaacaacgaa atgaactcct
      421 tctgatggtc agtcatggtg gccatgtttt ctccgtaaaa acagggaaca catggcccgg
      481 acaggggaac aggtgttccc cttcgggttg aaatgttgat aaaacgggca tcacggacat
      541 gatacccgga ggttaattac tgggctgaga ccagaggaac cggtaaaggt gcggctttcg
      601 tctgttcttc agcgcactcc tccaccag
//
LOCUS       DAZGAP010000549          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZGAP010000549 DAZGAP010000000
VERSION     DAZGAP010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.552"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..445)
                     /locus_tag="ACHLAM_005558"
     CDS             complement(<1..445)
                     /locus_tag="ACHLAM_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO7856889.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQLGNVKALFESKPFITLSEPACGAGSMILAM"
BASE COUNT          170 a          170 c          139 g          149 t
ORIGIN      
        1 ccattgccag aatcatactg ccggcaccac aggcaggttc actgagcgtg ataaagggtt
       61 tactctcaaa caacgccttc acatttccca gttgcatctg tgccatcatg ctggcaacac
      121 tccagggcgt gaaaaactga ctccggtatt tatcccccag ttccagttgc atgaacacac
      181 tacccagaaa atcacagaat ccctgctcca gtccctcaat gacatgagca agcaaatgcg
      241 caatgtggat aacatcctgc ttttcataac cactgacgat tcgcagatac ttctgttccc
      301 gggcctcact gaactgaagc cggttttcca gggcaatcac actacagctg acaaagtctt
      361 caaacacctt atgacgtttg tgataacagg ctgtccggtt aaacaacgaa atgaattcct
      421 tctgatggtc agtcatggtg gccatgtttt ctccgtaaaa acagggaaca catggcccgg
      481 acaggggaac aggtgttccc cttcgggttg aaatgttgat aaaacgggca tcacggacat
      541 aatacccgga ggttaattac tgggctgaga ccagaggaac cggtaaaggt gcggctttcg
      601 tctgttcttc agtgcactcc tccaccag
//
LOCUS       DAZGAP010000550          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZGAP010000550 DAZGAP010000000
VERSION     DAZGAP010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.553"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>628
                     /locus_tag="ACHLAM_005559"
     CDS             <1..>628
                     /locus_tag="ACHLAM_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO7856890.1"
                     /translation="QFADDYTVTTSNDSSWSGGGIIVNYGTTVKWGINGVSGDDLHKV
                     GDGTLIINGTGKNEGGLKIGAGTVILEQKAKNNDSTAFSSINISGGNSRVKLSGDNQI
                     IPDNVSWGFRGGYLDINGKDTEFSRLQAVDYGAAIINSSTDKSLLTLNLSPLKKDEIA
                     VSVKALDMNAIFQGGHGTAGDLYKTTFYGPTQYYLLKKPKFGSVLMGSL"
BASE COUNT          197 a          106 c          161 g          164 t
ORIGIN      
        1 ttcagtttgc tgatgattat acagtaacaa cgagcaatga cagttcatgg tccggaggag
       61 gcattattgt caattatggt acaactgtaa aatggggtat aaacggggtt tccggagatg
      121 accttcataa agttggggac ggaacgctta ttattaatgg aaccgggaaa aatgaagggg
      181 gactaaagat tggggctggt actgtaatcc ttgaacaaaa agcgaaaaac aacgattcaa
      241 cggctttttc ttccattaac atcagtgggg gaaactcgcg cgttaaactt tcaggagata
      301 atcagattat acctgataat gtttcctggg gattccgggg gggatattta gatataaacg
      361 gaaaagacac tgagttctcc cggttacagg ctgttgatta tggggctgca attattaaca
      421 gcagcacaga caagtcatta ttgacgttaa atctgtcacc attgaaaaaa gatgaaatag
      481 ctgtaagcgt gaaggcgtta gatatgaacg ccatctttca ggggggacac gggacagccg
      541 gagatttgta taaaacaact ttttacggtc cgacacagta ttatctgctg aaaaagccca
      601 aatttggcag tgtgctgatg gggtcact
//
LOCUS       DAZGAP010000551          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZGAP010000551 DAZGAP010000000
VERSION     DAZGAP010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.555"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          201 a          128 c          117 g          179 t
ORIGIN      
        1 aaaaaataag aaccatgtca caaaacatta cattccggca agttatgaca gaattactga
       61 aaagcgttat ctgtagtaat tgtagtttct gatatatgct catccatccg gaaatacgac
      121 atggtcctga ctggcgttca cttcaaagtg taatttatta ttcacacact gagactgaaa
      181 aacagatggc gtggatgagt acacagcctg aagagtataa aaggaacagc tccggattgt
      241 tacccgggat tactatagtt caccacacaa ccgtaaacct ggttgtgtgg tgaaacagta
      301 acatattaag aatattgtgt taccacctgt tttctcagga aacggattgc aggagaggta
      361 tactcattca tcttcccgga gttttgttaa tcactgcata atcaaacgga ccaatacatt
      421 ctttgctcgc atccgtaaaa atgtcgccac aatatcatca gtcagtcaac tcattaccat
      481 ctgttttccg gggccgtagt tactacagtg ctccggagtg ataaacagaa actgataatg
      541 aagcataaga atacttttat tatgcgctga ctgacatact ttacttaatt gcgattctgc
      601 tctcaataaa tccggactga ttaat
//
LOCUS       DAZGAP010000552          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZGAP010000552 DAZGAP010000000
VERSION     DAZGAP010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.556"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..512
                     /locus_tag="ACHLAM_005560"
     CDS             <1..512
                     /locus_tag="ACHLAM_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7856891.1"
                     /translation="DMILDQEWILASDLLGVGGLPSSLAGLHKRAKNEGWEKRSVTTP
                     GIRGRAFAYRLSDLPAHVVSIITGSAEIKHQPPQKNGGNSELLELIDTLSQEEQELVI
                     YTLRRKGVEKLLEFCNQENQELIALTGIRRLAALSLNNLSDQKVREIFEGDETKDHHF
                     NLTHKEAKA"
BASE COUNT          200 a          121 c          137 g          157 t
ORIGIN      
        1 ttgatatgat ccttgaccaa gaatggatcc ttgccagtga cttgttgggg gttggaggtc
       61 ttccttcatc tctggctggg cttcataaac gagcaaaaaa tgaaggatgg gaaaaacgtt
      121 cggtaactac acctggtatc agagggagag catttgccta ccgtttaagc gacttacctg
      181 cacatgttgt ttccatcatt acaggtagtg cagaaataaa acaccagccc cctcaaaaga
      241 acggtggcaa tagcgagttg ctcgaactca tcgacacttt gagccaagaa gagcaagaat
      301 tagttattta caccttgaga aggaaagggg tagaaaaatt gctggaattc tgcaatcaag
      361 agaatcaaga gttgatcgct ttaacaggta ttcgacgcct ggcagcattg agccttaata
      421 atttatcaga tcaaaaggtt agagagattt tcgagggaga tgaaaccaag gatcatcatt
      481 tcaatttaac ccataaagaa gccaaagcgt aaccccatga agcccaggcc atgcacttag
      541 cgagcacttt caacttgatt tgtgaccaga tcgatattga tataaacttt ttagcataaa
      601 agacatttta agtac
//
LOCUS       DAZGAP010000553          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZGAP010000553 DAZGAP010000000
VERSION     DAZGAP010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.557"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>611
                     /locus_tag="ACHLAM_005561"
     CDS             2..>611
                     /locus_tag="ACHLAM_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO7856892.1"
                     /translation="MNYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLY
                     LLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLV
                     DESGRVIFADFNPSKRPWSMASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLL
                     VVDKIHVDLNWRYLNEYLDHISANDEVLFLKQGHEIIAKNQLA"
BASE COUNT          186 a          116 c          133 g          176 t
ORIGIN      
        1 cattaactat caactggtat cggaacgcgc ggtaaaacgt gccgatagcc gctttgaact
       61 tattcagaaa aacgttggct atttctttaa agatattgaa cgttcggccc tgacattaaa
      121 ggactcacta tatttattaa aaaatacaga ggagattcaa cgcgccgtaa ttctgaaaat
      181 ggaaatgatg ccatttttag actcggtggg actggtactt gatgataata aatattatct
      241 cttttcgcgg agggcgaatg ataaaatcgt tgtttatcat caggaacaag taaatggacc
      301 gcttgtcgac gagtcagggc gggttatttt tgccgatttt aacccatcga aacgaccgtg
      361 gtcgatggct tcagatgact ctaacaacag ctggaatccg gcatacaatt gctttgatcg
      421 tccgggtaaa aaatgtatct cttttacgct acgcatcaac ggcaaagatc acgatttgtt
      481 agtggtggat aaaattcatg tcgatttaaa ctggcgatat ctgaacgagt atcttgatca
      541 catcagcgct aatgatgaag ttctattttt aaagcaaggt catgagatca ttgccaagaa
      601 tcaactcgcg c
//
LOCUS       DAZGAP010000554          607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZGAP010000554 DAZGAP010000000
VERSION     DAZGAP010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.558"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..>607
                     /locus_tag="ACHLAM_005562"
     CDS             151..>607
                     /locus_tag="ACHLAM_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO7856893.1"
                     /translation="MSILTTGAFLALAAQCAPNVHPDTSLDVVRVESGLHQYAIAEII
                     PKNERVAEGKSFISHYPESKDEAVKIIRRIEERKRNYSVGLMQINSSNFKKLRVTATE
                     LLEPCSNLKAYQYIITDCYLRGGSLTNALSCYYSGDFITGHKVESELNNT"
BASE COUNT          203 a           90 c          136 g          178 t
ORIGIN      
        1 ggaatttatt aaaggaataa atgatgagtg tcaattactt gtaaagccaa catatattgt
       61 tcattgtctt ttaaactgtg cattacagag cgaaggaatc aggaaagaag ttattaatta
      121 tgttgctaat gcacgagaaa aaatgcgctg atgagtatac tgactaccgg ggcatttctc
      181 gctctggctg cacagtgtgc cccaaacgtt catccagata catctcttga tgtggttcgt
      241 gttgagtcgg ggttacatca atatgctata gcggaaatta tacctaaaaa tgaaagggtt
      301 gcagagggta aaagctttat aagtcattac ccggagagta aagatgaagc agtgaaaata
      361 atcaggagga ttgaggaacg gaaacgaaat tattctgttg gattaatgca gattaacagt
      421 tcaaatttca aaaaacttag agtgacagct actgaattgt tggagccgtg ctctaatctg
      481 aaagcgtatc aatacattat taccgattgt tatttgcgag gaggtagcct tactaacgcg
      541 ttaagttgtt attattctgg ggattttatt acaggccaca aggttgaatc agaattaaat
      601 aatacaa
//
LOCUS       DAZGAP010000555          607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZGAP010000555 DAZGAP010000000
VERSION     DAZGAP010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.559"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(10..282)
                     /locus_tag="ACHLAM_005563"
     CDS             complement(10..282)
                     /locus_tag="ACHLAM_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO7856894.1"
                     /translation="MKTSDNESTKYAITGQAVLQILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAITDNKSIFNGKKINRIH"
     gene            complement(325..561)
                     /gene="ycgZ"
                     /locus_tag="ACHLAM_005564"
     CDS             complement(325..561)
                     /gene="ycgZ"
                     /locus_tag="ACHLAM_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO7856895.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRIL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          169 a          126 c          102 g          210 t
ORIGIN      
        1 tatgaaattt taatgtattc tgtttatttt cttaccattg aaaatacttt tattgtcagt
       61 tatcgctgtt cttctgtttg gccctcctgt atcactgttg ctaaaatcac gaattatgct
      121 gtcaattaaa tctcgtcgga aagcattttc ctcaacagat ttcattacga gtaattgctt
      181 aataagagtc tgcaacgaaa aatttatctt catgcgcagt atctggagca ccgcctggcc
      241 agtaattgca tattttgtac tttcattatc agatgtcttc ataacgactt catagctaac
      301 ctgttaaata atcaggctgt tgcattattc aaaaagcaac ccaattagtg cattataatg
      361 tttctgttcc tcttcccctg tcgcttgttc cagtcggcat agaagtttgg cacaaagcga
      421 ctttcgactc agaatacgtc catctttcaa aatctcagtc acaatttccc cgagagtttc
      481 ctgctgagta ggcaagttag cttttgcaaa gtaacgagtg attgctcccg cagaatctaa
      541 agtcactgaa ttttgatgca tgctacgcct ctgttaaaaa tgttaatcaa tgttaagtga
      601 ggtaaca
//
LOCUS       DAZGAP010000556          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZGAP010000556 DAZGAP010000000
VERSION     DAZGAP010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.560"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..143)
                     /locus_tag="ACHLAM_005565"
     CDS             complement(<1..143)
                     /locus_tag="ACHLAM_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011146240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7856896.1"
                     /translation="MLKNLLNTKVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHS"
     gene            414..>602
                     /locus_tag="ACHLAM_005566"
     CDS             414..>602
                     /locus_tag="ACHLAM_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7856897.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAG"
BASE COUNT          174 a          126 c          146 g          156 t
ORIGIN      
        1 gagtgataga tcgcatcaac ataacgcggt tcgatagagc cgttttcaat caacggtcgg
       61 catgaaatcg ccacggcttc ccgccagtct ttgacctgtt ccacgacctg aacgaccttt
      121 gtatttaata gattttttaa catgcgactg ataactcccg atgataatta acaggagagt
      181 agatatttta cgcattaatt aatgtgatga ttgtcgcatt tgatagcgct atctgataac
      241 gctaccaatc tttgtgatag cgctatcatt tctctgccgc catgctttgg ctatattcgg
      301 ttcctgcata tacaatgcaa caggatattt gctaccgtgg gcaaaaactg gaaagagtaa
      361 tggtgaaata aaagtaatga aaacagctgg ttgtaattat ccatggagtc gaaatggaaa
      421 aaacacgtaa gcgccgtggt accggacggg tgacgctgca ggaggtcgct aactttgcag
      481 gtgtcggaac catgaccgtt tcccgcgcgc tgagaacacc ggaacaggtt tctgacaagc
      541 tgagggaaaa gatagaacag gccgtagaag agttgggtta tattcccaac agaactgctg
      601 gc
//
LOCUS       DAZGAP010000557          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZGAP010000557 DAZGAP010000000
VERSION     DAZGAP010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.561"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>593
                     /locus_tag="ACHLAM_005567"
     CDS             <1..>593
                     /locus_tag="ACHLAM_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856898.1"
                     /translation="LAKLGFRTEIAEPTSFDYLDGKKQPTGFFRSLINSLYEAANDDT
                     RTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFKK
                     DDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKLGPLLWHMHPIMFLGCMK
                     KSSGIWPLGKTSERYESGGRGPGVISSGKGDHGGVSY"
BASE COUNT          179 a          121 c          149 g          144 t
ORIGIN      
        1 tctggcaaag ctggggttca gaactgaaat cgcagagcct accagctttg attacctgga
       61 tggaaaaaaa cagcccactg gattcttccg cagtcttatc aactcactct atgaggctgc
      121 gaacgatgat acgcgaacca gtcatgcgct ggtgaaacat aattatcagc gcctgctgga
      181 taaaattgat agtggaagcg accgttactc tccaatggaa tactggagag cacttcataa
      241 ccccgactat cgtgatgttc ttcagaaaac aatcgttaaa catcccagcg actggtactt
      301 caaaaaagac gatgcaatct ggcaaccatt tctgaatgcg ctgaaaaaag atgctccgga
      361 atggaagaaa tacagcgaag attttcttga taagatggcc tggatgcagg atgtgaccac
      421 ggagaaactg ggccctttgt tgtggcatat gcatccgatt atgttcttag gctgtatgaa
      481 aaaatcgtca ggaatatggc cacttgggaa aacttcagag cgttatgagt ctggaggaag
      541 agggccaggt gtaatttcat ctggaaaagg agatcatggc ggtgtgtcat atg
//
LOCUS       DAZGAP010000558          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZGAP010000558 DAZGAP010000000
VERSION     DAZGAP010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.562"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>587)
                     /locus_tag="ACHLAM_005568"
     CDS             complement(<1..>587)
                     /locus_tag="ACHLAM_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7856899.1"
                     /translation="ILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRL
                     AALWQALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLV
                     DRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPS
                     AFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPDNLP"
BASE COUNT          103 a          210 c          175 g           99 t
ORIGIN      
        1 ggcaggttgt ccggatattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg
       61 ttgtcccggc catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc
      121 ccgccggaaa tggcctgctg ccgggcctct tctgcccgct gcgcctgcgt ggtcagtacc
      181 agccggaagt gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcgccg
      241 ccagcctcac ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc
      301 cggtacggcg gcagcggcgc aggcagcacc tcatccgcct ccggcacccg ctcacaccag
      361 ccgagtaccc accacggccc ctgcggactg ttcgtcgcca gataacgatg cggactcagg
      421 cgaagctctt ccggcagcgc ctgccagagt gcggccagcc gatgaccttc atccagtttc
      481 gccacgccgc cgcgcaccag ccagagtgac tcgctgcggc tgtaaccgtc ctcaccggga
      541 aacaggtgct caaaatacag gcttctgccg ccgttatcac tgagtat
//
LOCUS       DAZGAP010000559          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZGAP010000559 DAZGAP010000000
VERSION     DAZGAP010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.563"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(83..>587)
                     /locus_tag="ACHLAM_005569"
     CDS             complement(83..>587)
                     /locus_tag="ACHLAM_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO7856900.1"
                     /translation="SQGARSVMLEMIVLSATESVVGMVNPVLPDVREFIRGTVNVMEN
                     LMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKVLPEGAVTLLSTQ
                     IYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLACELLPEWREFFDALLENGG
                     KSLWAEN"
BASE COUNT          135 a          176 c          146 g          130 t
ORIGIN      
        1 tttttcgctt ctgcgcggtc agatgcatca acaaacacat gcacttcctc gtgtccgggt
       61 gctgaagcca cggcacgcca tatcagtttt ctgcccacag gcttttccct ccgttttcca
      121 gcagagcatc aaaaaactca cgccactccg gcaacagttc acaggccaga ctgtgcatgg
      181 tgaccagcgc agaatcgctg caacgggcag acactttttc cagacgatgc accggctgac
      241 cggctgcgtg cccctgttta taaatattca ggtcgtaaat ctgtgttgaa agaagggtga
      301 ccgcaccttc aggcagcact ttgctgtagc gaccggcgtt gataatgcct gtcagctctg
      361 ccagtgtctg tctggccagc gccgtataat ccatgcagtt aaccagggtg cggactttcg
      421 gcaggggaat acccagttca cggtagggca taaggttttc catgacattg accgtgccgc
      481 ggatgaactc acgcacatcc ggcagaaccg gattcaccat ccccaccaca gactccgtgg
      541 cggacagcac aatcatttcc agcatgacag aacgtgcgcc ctgtgaa
//
LOCUS       DAZGAP010000560          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZGAP010000560 DAZGAP010000000
VERSION     DAZGAP010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.564"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>582
                     /locus_tag="ACHLAM_005570"
     CDS             <1..>582
                     /locus_tag="ACHLAM_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HFO7856901.1"
                     /translation="IGYGFIFPDSYFDDKIINAAMVDASVKPFRGIFVDKSQLPEMAK
                     HTIDIMQYWKHALGNNELGDERVQLIIKSVCPEFDGTPDWGIRIIYDNKLWLRLTHEQ
                     IKVIELLSEYQRVVVTGWPGTGKTLIGIEFARRLVKGQKKVLFITFNNLLSEYIRQQT
                     PESLCDVFTWHKLCHQARNKLSLSPESPAGWFES"
BASE COUNT          181 a           99 c          125 g          177 t
ORIGIN      
        1 attggttatg gttttatttt tcctgatagt tatttcgatg ataaaattat aaatgcagca
       61 atggtagatg cgtctgttaa accattcagg ggaatatttg ttgataaaag ccaactcccg
      121 gaaatggcaa agcatactat tgatataatg caatactgga aacatgcatt gggaaataat
      181 gagttggggg atgaacgggt tcaactaata ataaaatctg tatgtcctga gtttgatggt
      241 actcctgact ggggaatccg tattatttat gataataaat tatggttaag gctcacacat
      301 gaacaaatca aggtcattga acttctgtca gaatatcaaa gagttgttgt aaccgggtgg
      361 ccaggaacag gaaagaccct gattggaatt gagtttgcac ggcggcttgt taaaggccaa
      421 aaaaaggttt tatttattac tttcaataac cttctttcag aatatattcg gcaacaaact
      481 ccggagtcat tatgcgacgt ttttacctgg cacaaacttt gtcatcaggc tcgaaacaaa
      541 ttatccctgt cccccgagtc tccggcaggc tggtttgaat ca
//
LOCUS       DAZGAP010000561          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZGAP010000561 DAZGAP010000000
VERSION     DAZGAP010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.565"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..>582
                     /locus_tag="ACHLAM_005571"
     CDS             78..>582
                     /locus_tag="ACHLAM_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856902.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     IKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDL
                     AQSAAAVK"
BASE COUNT          193 a          109 c          124 g          156 t
ORIGIN      
        1 tgcttatgaa tattaccagg aaatagccac attactattt aattatctga gataaactca
       61 acgtcaagag gtgggttatg attagatata gtggaatatt tctatcgctg ttaatgcttt
      121 caggttgtgc aactgaagca ttgaaaaacg aacaagctga aactgttatt actgcgggta
      181 acaaagcagt agcggcgaat gcaagtttca ttaaacaata ctatgcttct cgtgaaaact
      241 tccttgttaa tttctacgcg actaatccgg attgtgccct gcgacctgat tataccattg
      301 atataggcgg tgatgggaat ggcggaaaat tatgccagcg ttcagggcca aatacaaaac
      361 aagtacaatt agtccctttc gagaaagaaa aattactggc ctcaatggaa gtcattaata
      421 gcatgagcgc ttatgtggaa cttctgacaa aggatatggc aactacggaa agtcagcaag
      481 tggcattact taaacaggct ctggatcacg caaacaacgc acaaacacta cttggtgttt
      541 cacatagcaa agatttagct caatctgcag cagcggttaa ag
//
LOCUS       DAZGAP010000562          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZGAP010000562 DAZGAP010000000
VERSION     DAZGAP010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.566"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>581
                     /locus_tag="ACHLAM_005572"
     CDS             <1..>581
                     /locus_tag="ACHLAM_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7856903.1"
                     /translation="TGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAA
                     KTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQ
                     LKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGE
                     GREIDFRNTVILMTSNLGSDLLMQQLSEKPETT"
BASE COUNT          132 a          142 c          191 g          116 t
ORIGIN      
        1 ggaccggggt gccgttatct tccctgatga aggacgaaca gacggaactg ctgcatctgg
       61 aaaaggatat cggcagacgg gtggtcggac aggacgtggc actggaatcc attgcgcagc
      121 gtctgcgtgc ggcgaaaacg ggtctgacat ccggtaacgg cccccagggg gtgttcctgc
      181 tggttgggcc cagtggtgtg ggaaaaaccg aaacggcact ggcgctggca gacgtgatgt
      241 acggcggcga aaaatcactg attaccatca atctgtcgga ataccaggag ccccatacgg
      301 tttcccagct taagggttca ccgcccgggt acgtgggata cggtcagggc ggtattctga
      361 cggaagctgt gcgtaaacgc ccttacagcg tggtgctgct ggatgaagtg gaaaaggccc
      421 accgggatgt gctgaatctg ttctaccagg tgtttgaccg gggctttatg cgcgacggcg
      481 agggacgtga aattgacttc cgtaatacgg tcattctgat gacctccaac ctgggtagtg
      541 accttctgat gcagcaactg agcgaaaagc cggagacaac g
//
LOCUS       DAZGAP010000563          579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZGAP010000563 DAZGAP010000000
VERSION     DAZGAP010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.567"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>579)
                     /locus_tag="ACHLAM_005573"
     CDS             complement(<1..>579)
                     /locus_tag="ACHLAM_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HFO7856904.1"
                     /translation="VIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCVLIFLALVNKV
                     SENTKQRNLFSKKVTLCITLFFVLGGPIVAHIFSSHYESYDLHIAELTNKNGQVVWKA
                     SYVTIMIFMWLTLLSVNLYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYI
                     SRTIEVVSKLTVLVIFMCHIFSALRVTKDIAH"
BASE COUNT          211 a          124 c           91 g          153 t
ORIGIN      
        1 gatgtgcaat gtcctttgtt actcgtagcg cactgaatat atggcacata aaaattacca
       61 aaacggttaa cttactgact acttcaatag tgcgactaat ataccaaatg gacacgctat
      121 acctgctcat aaataacaga gagatattgt acagcacggc acaaaaagca atcaccgaca
      181 ccccgttcca gatatcacag cgtaaaccat taaagtatag attaaccgaa agcaaggtta
      241 accacatgaa aatcattatg gtaacatatg aggctttcca aaccacctga ccatttttat
      301 tggttaactc ggcaatgtgt aaatcgtagc tctcataatg gctagaaaat atgtgagcaa
      361 ctatcggccc cccaagaaca aaaaacaaag ttatgcacaa agtcactttt ttggaaaaca
      421 aattgcgctg ttttgtgttt tcactaactt tatttaccaa tgccagaaat attaatacgc
      481 acaaactcat ctgacgaaac aaataataaa ttgcaatgtc attttgaaca acactgcttg
      541 tcaaacgccc ctcaataatt tgctgaatga taatgacgg
//
LOCUS       DAZGAP010000564          574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZGAP010000564 DAZGAP010000000
VERSION     DAZGAP010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.568"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..410
                     /locus_tag="ACHLAM_005574"
     CDS             129..410
                     /locus_tag="ACHLAM_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856905.1"
                     /translation="MKTNKKGTTWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
BASE COUNT          193 a          110 c          106 g          165 t
ORIGIN      
        1 tactaaatca ctggctacat cgtatacata aaacagatat cattgttctg tctgttgttc
       61 ttacagaata aatcctgtta cctcctgaat gttacaaggt tttatattta tcctatgaga
      121 atacgcacgt gaaaactaat aaaaaaggca caacatggca cattttttac cgtgaaaatt
      181 caggcgcgga agttttgctt gaaataccat catttcggga atgtctttca gtatccaaag
      241 aattaatgac cccatcaaat tacatgatat gcatagaaaa aaatggcgaa agaataaaac
      301 gctgggacag ggaaatcatc gctggctcta acaaatggat taactgccca cctgataact
      361 tcgaaatatt aggtgagcta ataaccatta acagaattat taaaaagtaa ctccggattc
      421 agacaatgca ttaagcaagg cgctatagtt cattttttgc gggagaatgc ttgtaagtca
      481 tcgctgtgcc cggacctttc atccgcaaga gtttgttagc tggacgccaa actggtttat
      541 taccggagac tgagttcagt actcttaaat acat
//
LOCUS       DAZGAP010000565          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZGAP010000565 DAZGAP010000000
VERSION     DAZGAP010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.569"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(286..427)
                     /locus_tag="ACHLAM_005575"
                     /pseudo
     CDS             complement(286..427)
                     /locus_tag="ACHLAM_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          166 a          125 c          121 g          156 t
ORIGIN      
        1 ctttttcccg gcaatctctg tttcaaacgg acggttaaca agaataacgt aacagacaat
       61 catcacacat actccctgat tagcataaaa acaacgatat caaaagacta gacgcttttt
      121 caggaaaaat cttatttgtc cggagattat tcttacgggg ccgggcgaat gctatctgaa
      181 tcgtacggtt catagaaata aatatttacc gacatagccg gagggttttc agatgcaatc
      241 cctgagtcac tgcgaaatcg catcgtatcc gatcgcggac tcgccgattc tcagaacagg
      301 cagtccctga gaatcgcaga acgctagtgc acgatcgttg aacacatcag ctacggtgat
      361 cgatgtttta ctcgtattat agtttgcagt gtgccacttt cggggatgta gctacgaacg
      421 tctgctggct tcccgatatg tcagtaaaaa atacccgccg tattacagca acggcaggta
      481 atggcacatc cagaagagaa gtattttatt atatcctcat ttttatcatg tggtgtcagg
      541 agaattcaat tcggaataag tttgcatt
//
LOCUS       DAZGAP010000566          567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZGAP010000566 DAZGAP010000000
VERSION     DAZGAP010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.570"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>567
                     /locus_tag="ACHLAM_005576"
     CDS             <1..>567
                     /locus_tag="ACHLAM_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO7856906.1"
                     /translation="KNGNVTLSSEGNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGL
                     KADDAEMKNAASVADLKTAIDGVSTSMQGAGFALKDSKGQEVKQSLGQAIKVSGDSNI
                     TTTAITANGDKGLKISLNNDLTVGDKAPGSIKVNGADGKEAVSLNGQDGTIKLTSSPA
                     AGGKSASATIAVNAGKGGLTDAAGTTKTR"
BASE COUNT          170 a          111 c          168 g          118 t
ORIGIN      
        1 aaaaacggaa atgtaacgct ctcatccgaa ggtaataagc tgacattgtc aggcggtaat
       61 ggtgccggac aaaatggtgt tgttatcagt ggtgtggcaa gtggattgaa taataaggac
      121 ctggcaggcc tgaaagcaga cgatgcggaa atgaaaaatg ccgccagtgt tgcagacctg
      181 aaaacggcaa ttgatggtgt gtccaccagt atgcagggtg caggtttcgc actgaaagat
      241 tctaagggac aggaagtgaa acagtctctt gggcaggcga ttaaagtatc cggggacagc
      301 aatatcacca ccacagcaat cactgccaat ggagataagg ggttaaaaat atccctgaat
      361 aatgacctga ctgtggggga taaggctccc ggcagcatca aagttaacgg ggctgatggt
      421 aaggaggctg tgagtctgaa tggtcaggat ggcacgatta aactgacatc ctcgcctgct
      481 gcgggtggaa agtctgcgtc agcaaccatt gctgttaatg caggcaaagg tggattaaca
      541 gatgctgccg gtaccacgaa gacacgt
//
LOCUS       DAZGAP010000567          567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZGAP010000567 DAZGAP010000000
VERSION     DAZGAP010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.571"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..>567
                     /gene="dolP"
                     /locus_tag="ACHLAM_005577"
     CDS             41..>567
                     /gene="dolP"
                     /locus_tag="ACHLAM_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7856907.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGAQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVAS"
BASE COUNT          146 a          122 c          169 g          130 t
ORIGIN      
        1 gggaatatga agtaaaaatc aaaacaattc cgggggagat atgcgtttat tatattctgc
       61 tgttgtcctt acttcagcct tattgttaca gggatgcgtg gctgccgctg tggtgggtac
      121 agctgctgtc gggacaaagg cagcaaccga cccacgcagt gtcggagcgc aggtggatga
      181 tgaagtgctg gagctgaggg tcagcagtgc actttcaaaa gatgaacaaa tcagaaaaga
      241 agcacgcatc agtgagattg catatcaggg acgcgtgctg ctgatcggtc agtctccgga
      301 ccgtgaatta tctgcccgtg cccgtcagat agcactcggt gtcgatggtg ttcaggaggt
      361 ctataatgaa atccggcagg ggacacccgt cagtccggtc ataatggcag gtgatgcctg
      421 gcttacgaca aaaattcgtt ctcagttact cacaagtgcc cgtgtcaaat catccagtgt
      481 caaggtggtg acagaaaaca gcgaagtctt tttactgggc ctggttacag agcaggaagg
      541 gcgagcggca gcagatgttg ccagtag
//
LOCUS       DAZGAP010000568          556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZGAP010000568 DAZGAP010000000
VERSION     DAZGAP010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.572"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>556
                     /locus_tag="ACHLAM_005578"
     CDS             <1..>556
                     /locus_tag="ACHLAM_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO7856908.1"
                     /translation="AALTGFSGTEVGGCYPLDPTELNIELALRHLEIPGKWFSSFIIR
                     DGIESVKKAFEQHCAYLQAVHAYIAVVSEQTYSIQGIIDKCVYTEKPYFNDIEWQQLC
                     SGLNELGMIANTYGLKLAFHHHMGTGVQTLQEVDRLMENTDPQLVNLLFDTGHIYVSD
                     GDVMSLLCKHFSRIKHVHFKDVRDE"
BASE COUNT          158 a           96 c          122 g          180 t
ORIGIN      
        1 tgcagcactt actggttttt ctggaacaga agtgggggga tgttatcctc tggacccgac
       61 tgaattaaat atagagttag cacttcgcca tctggaaatt cccggaaagt ggttctcatc
      121 ttttattatt cgtgatggta ttgaatcagt gaagaaagct tttgaacaac attgcgctta
      181 tttgcaggct gtccatgctt atatcgctgt tgtgtctgag caaacgtata gtattcaagg
      241 aattattgat aaatgtgtct atacggaaaa accttacttc aatgatatcg aatggcaaca
      301 attatgctca ggattgaacg agttaggcat gatcgccaat acatatgggc ttaaacttgc
      361 tttccaccat catatgggca ctggcgtaca aactttacag gaagtggatc gacttatgga
      421 aaacacagat ccacaattag tgaatttgct gtttgatact ggtcatattt atgtttctga
      481 tggagatgtg atgtcactgt tgtgcaagca tttttcccgt attaaacatg ttcactttaa
      541 agatgttcgg gatgaa
//
LOCUS       DAZGAP010000569          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZGAP010000569 DAZGAP010000000
VERSION     DAZGAP010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.573"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(14..165)
                     /locus_tag="ACHLAM_005579"
                     /pseudo
     CDS             complement(14..165)
                     /locus_tag="ACHLAM_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          143 a          131 c          105 g          174 t
ORIGIN      
        1 atgtatcttc ctgttaattt aaggtcaact gcctgacgtt tctctccgaa tattccatta
       61 tattaatgtt gacttgttga ccagctgcgc cctgtatgct ggcatcaact ctattttaga
      121 ctgaacacgc cactcagttt cctccctttg cgacgcagcc tgcatttaca ctcaaactgt
      181 taagatgata aatgtggtaa atctgttggt actgacataa aaacgtttac gccacaggaa
      241 cggccagatc catcggtaac cccatcgccg acgttcgagt gccagttaga gtaacgcgta
      301 cagatgactg aatgcagtgc cctggcaaag aggccatcgt tcctgtgaca actggcagtc
      361 ttcgtttaac ttcacttaat ttggctcttg gggggcttac cggacagatg acgtacttac
      421 acctgtttaa tttttcatca cttattggga tgaacaccca caaccatttt gtgcggcatg
      481 ttaatccatt aaaacacctt actgattggc aaatcatctt taattatgac ttatgatagt
      541 tttatattcc att
//
LOCUS       DAZGAP010000570          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZGAP010000570 DAZGAP010000000
VERSION     DAZGAP010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.574"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>545
                     /locus_tag="ACHLAM_005580"
     CDS             <1..>545
                     /locus_tag="ACHLAM_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7856909.1"
                     /translation="SWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIV
                     GVPGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSR
                     AIRRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFD
                     DLSRLFSRLARTKQLRKAKKA"
BASE COUNT          118 a          145 c          170 g          112 t
ORIGIN      
        1 tcctggtatg agaactggaa taaacaaagc gcactgccgg aaccgccgca gtttgtggaa
       61 acgcagacag ttcaggagct gcgcgcactg tttcaggcgg ttcgcctgat gggctgtatt
      121 aacgtcattg tgggcgtgcc gggggtaggt aaaacggcca ccgcccgtaa ttactgccag
      181 gagcaaccaa acacctggat gatcaccctg tcacccgcgc actccagcgt cacggagtgt
      241 ctgctggagc tggccgatgc gctggggatt gattacaccc gcgcgaacaa aggggcatta
      301 tcccgcgcca tccgccgtcg cctgatggga acgcgtggac tggtgattgt ggatgaggcg
      361 gatcatcttg gtattgacgg tctggagcaa ctccgggcaa ttcaggacgc cacggggatc
      421 gggatggtgc ttattggtaa cccgcgcgga ttgtttaaag gtggacgccg cgcctttgat
      481 gatttatcgc gcctgttcag ccgtcttgcc cgtacaaaac aacttcgcaa ggccaaaaag
      541 gcgga
//
LOCUS       DAZGAP010000571          540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZGAP010000571 DAZGAP010000000
VERSION     DAZGAP010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.575"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..143
                     /locus_tag="ACHLAM_005581"
     CDS             <1..143
                     /locus_tag="ACHLAM_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO7856910.1"
                     /translation="ASAMKYHIMNGYSYKEALALTSIDLGHGDGRGRYIEQVYCQLLG
                     YE"
     gene            176..499
                     /locus_tag="ACHLAM_005582"
     CDS             176..499
                     /locus_tag="ACHLAM_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856911.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
BASE COUNT          164 a           74 c          127 g          175 t
ORIGIN      
        1 ctgcaagtgc catgaaatat catattatga acggttacag ttataaggag gcacttgcac
       61 tgacatcaat tgatcttggt catggtgatg gcagaggacg atatattgaa caggtttatt
      121 gtcagttgtt aggctatgag taataactca tggcgatttc gtacgggtaa atattatgga
      181 ttgtactatt ttgctgaaaa taatctctgt aaaagagatt acaaaaaatt cgcctttgtg
      241 gtcacactat ctggcgcgat atgttgattc cggatatcag gccgagtttg aatgtgaagg
      301 aacagcagat aaatgggata tctatgatga atgggacatc tatgaatcca ttgcggatta
      361 ttttgatgtg gtaggagatt ttaactgtga tgtcagatgg tcgggaagaa aaatactgat
      421 taaagggacc tgtgtttttc ctctgggaga acccgttaag ggaatacata atattcctct
      481 ttggattgat gttgaataac ttgacattca tcttttgtga tttaaaaata tacaggctta
//
LOCUS       DAZGAP010000572          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZGAP010000572 DAZGAP010000000
VERSION     DAZGAP010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.576"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>537
                     /locus_tag="ACHLAM_005583"
     CDS             <1..>537
                     /locus_tag="ACHLAM_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO7856912.1"
                     /translation="LWHMHPIMFLAFLIQPNEDIRILRLRAFLRMIRIGEGTIQEDGY
                     RTMFTGVKFTDFSKHPNIRHEANGVVSTAAGAYQFLYGTWRNLQRRYSFPDFSPSNQD
                     LGCIALIAGRKALDVVMQGKISEAIHLCRIEWASLPGSPHGQPTANKKMIMEKYELYL
                     AEEKEGKTSLHATTEKIV"
BASE COUNT          181 a           92 c          111 g          153 t
ORIGIN      
        1 gttgtggcat atgcatccaa taatgttttt agccttccta attcaaccta atgaagatat
       61 tagaattctt cgtttacgtg catttctacg aatgatccgc atcggtgaag ggacaattca
      121 ggaagacgga tacagaacga tgtttactgg tgtaaaattt acagatttta gtaaacaccc
      181 caatatcagg catgaagcta atggtgttgt ttcgactgct gctggagctt atcaattttt
      241 atacggaaca tggagaaatt tgcaacgtag atattcattt ccagatttca gtccgagcaa
      301 tcaagatctc ggttgtatag cgctcattgc aggtcgaaaa gcattagatg tagtaatgca
      361 gggtaagatt agtgaagcca tacatttatg tcgtattgag tgggccagtt tacccggttc
      421 accgcatggt caaccaactg caaataaaaa aatgattatg gaaaaatatg aactctactt
      481 agctgaagaa aaagaaggta aaacaagttt acatgccacc acagaaaaaa tagtaaa
//
LOCUS       DAZGAP010000573          535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZGAP010000573 DAZGAP010000000
VERSION     DAZGAP010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.577"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..69
                     /locus_tag="ACHLAM_005584"
     CDS             <1..69
                     /locus_tag="ACHLAM_005584"
                     /inference="COORDINATES: protein motif:HMM:NF036593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFO7856913.1"
                     /translation="PEKLHPSLMAIKHSEFIAITEE"
     gene            73..>535
                     /locus_tag="ACHLAM_005585"
     CDS             73..>535
                     /locus_tag="ACHLAM_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856914.1"
                     /translation="MIDAKVLEGVKNWLRIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGLLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNIT"
BASE COUNT          122 a          113 c          157 g          143 t
ORIGIN      
        1 cctgaaaagc tgcatccgtc attgatggca atcaagcatt ccgaattcat cgcaataacg
       61 gaggaataat tcatgattga tgcaaaagtg cttgaagggg ttaaaaactg gctgcgtatt
      121 tacggacgtc tgacctgcgg tgttctggct gaaaaaatga atatgccgcc atcctccatg
      181 gtttattttc tgcgtgatgc ggttgatgca ggcttgctga cggaatgtaa cggtttttat
      241 gatattccgc gtccccgccc ggtgcagccg gttcgtcgca aatgcagcca ggaaggtgcg
      301 gctgatgatg ttcagtggtg cagcttcaga aaatccctgc cgtggattga ggggcatgat
      361 attccgtcga tggcgtggga atttgctcag ggcgtactga cctgcgaaac cgtttatgtg
      421 gtggctgaag ttgatgagca ggccatgaaa gaaggcgtgc cccagtttgt gatggcgtat
      481 atcgacattc gcctgggtgt cattatctgc ggtttaagcg gctggaatat caccg
//
LOCUS       DAZGAP010000574          535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZGAP010000574 DAZGAP010000000
VERSION     DAZGAP010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.578"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..69
                     /locus_tag="ACHLAM_005586"
     CDS             <1..69
                     /locus_tag="ACHLAM_005586"
                     /inference="COORDINATES: protein motif:HMM:NF036593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFO7856915.1"
                     /translation="PEKLHPSLMAIKHSEFIAITEE"
     gene            73..>535
                     /locus_tag="ACHLAM_005587"
     CDS             73..>535
                     /locus_tag="ACHLAM_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856916.1"
                     /translation="MIDAKVLEGVKSWLRFSGRLTSRSLAEKMNMPLSSMVYFLRDAV
                     DAGVLTDRNGFYDIPRPRPVQPVRRKCSQESAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNIT"
BASE COUNT          117 a          116 c          155 g          147 t
ORIGIN      
        1 cctgaaaagc tgcatccgtc attgatggca atcaagcatt ccgaattcat cgcaataacg
       61 gaggaataat tcatgattga tgcaaaagtg cttgaagggg ttaaaagctg gctgcgtttt
      121 tctggccgtc tgaccagtcg ttctctggca gaaaaaatga atatgccgct atcctccatg
      181 gtttattttc tgcgtgatgc ggtcgatgcc ggtgtgctga cggaccgtaa cggtttttat
      241 gatattccgc gtccccgccc ggtgcagccg gttcgtcgca aatgcagtca ggagtctgcg
      301 gctgatgatg ttcagtggtg cagcttcaga aaatccctgc cgtggattga agggcatgat
      361 attccgtcga tggcgtggga atttgctcag ggcgttctga cctgtgaaac cgtttatgtg
      421 gtggctgaag ttgatgagca ggccatgaaa gaaggcgtgc cccagtttgt gatggcgtat
      481 atcgacattc gcctgggtgt cattatctgc ggtttaagcg gctggaatat caccg
//
LOCUS       DAZGAP010000575          531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZGAP010000575 DAZGAP010000000
VERSION     DAZGAP010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.579"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>531)
                     /locus_tag="ACHLAM_005588"
     CDS             complement(<1..>531)
                     /locus_tag="ACHLAM_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7856917.1"
                     /translation="SCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEA
                     CWSWLSDYPEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGN
                     NVRHARDEMSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDG
                     GEHTDSDLQAEWPGRET"
BASE COUNT          119 a          172 c          127 g          113 t
ORIGIN      
        1 gtttccctgc ccggccattc ggcctgcaga tcactgtccg tatgctctcc gccatcgtaa
       61 cagttgtaac cctgtatacc gtgtaccggc tcgctgtacc agtgtcggac ctcgcagtca
      121 aacaccttcg agatctcacc aaccagttcg cctgaaggcg gataccaggg cgtgtcgatc
      181 agcagcacca gactgctcat ctcatcacgc gcatggcgaa cattatttcc tgacggccac
      241 tccacaccgt atatccggct gtaaaggctg gtcgttgtgg aaataccctg cagcagtcca
      301 cctgaaccat tcagctctgt agccagacgg gagggtatga ccatcagcat gtcacagggc
      361 tgcgcctgtt ccgggtaatc cgacaaccag ctccagcagg cttctgcatc aatgtgttca
      421 ctcagcccgg ccagaccaaa ccagtcagaa tactgacggc tcatcagcgt ggtgatgatc
      481 tccctggcat tatccggcag attaccccag cgaacagcgc cgatacatga c
//
LOCUS       DAZGAP010000576          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZGAP010000576 DAZGAP010000000
VERSION     DAZGAP010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.580"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..395
                     /locus_tag="ACHLAM_005589"
     CDS             <1..395
                     /locus_tag="ACHLAM_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO7856918.1"
                     /translation="RIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYL
                     GRDRDSVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEK
                     FELSEGTVSFIYHHRRTAEDVVLRELMP"
BASE COUNT          136 a          112 c          138 g          142 t
ORIGIN      
        1 accggattgt tttattacgc ggtacttacc cggagttgct caaatgcaaa cgcctcagat
       61 ttaaacctga tgaagataaa tttatccgaa aaaatgccag gacgatgacc ggtaagcaaa
      121 taggagaata tctggggcga gaccgggact ctgtccacaa tcgggcgcga tatattggcg
      181 taagcatgaa aaaatacgga gagttgctgc ccttcacccg catacctgac gatgatgttc
      241 gtcttattcg tgaattacgg gatgctgaat caccacggcg tcttaccttt agggaaatcg
      301 gcgagaaatt tgaattatcc gagggcacgg tgagttttat ttatcaccat cgccggactg
      361 ccgaagatgt tgtattacgg gagttaatgc catgataacg acattatttg ttgaatcaga
      421 tgaacccctt gtgtgtgccg ccggaatgcc ggtctgtggc ggaacgctga ccggtgttta
      481 tttcggggat ttgcgcggtt atccctggca ttcactgaat gatgcttt
//
LOCUS       DAZGAP010000577          526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZGAP010000577 DAZGAP010000000
VERSION     DAZGAP010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.581"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..484
                     /locus_tag="ACHLAM_005590"
     CDS             <1..484
                     /locus_tag="ACHLAM_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO7856919.1"
                     /translation="IDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRAPELEN
                     RTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADL
                     IMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDGPGGYA
                     "
BASE COUNT          116 a          138 c          160 g          112 t
ORIGIN      
        1 aattgatgac tgctgtgagc agacgccttc atcactgcgt gcaggcgccc gtcgttatac
       61 acgtctgtac ggtaaaccct ccctgattat ggtggattat ctgcaactga ttcgtgcgcc
      121 ggagctggaa aaccgcacac aggaaattgc tgaaatttcc cgttccctga aagcgctggg
      181 gaaggagctg ggctgcccgg tgctggccct ctcccagctt aaccggcagg tggagcagcg
      241 tgctgataaa cgccccaata acggtgacct gcgtgattcc ggggcgctgg agcaggatgc
      301 tgacctgatt atgtttattt accgcgacga ggtctataac cccgactcac tggcaaaggg
      361 ggaggcagaa atcattgtca gcaagcagcg tcaggggccc acgggtacgg tacgggtcag
      421 gttcgacggt cgttacacac tgtttacgcc ctctgagaat gacgggccgg gaggatatgc
      481 gtgatggcac ataaaccact ggatctcggg gcggctatta tgcagc
//
LOCUS       DAZGAP010000578          520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZGAP010000578 DAZGAP010000000
VERSION     DAZGAP010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.582"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>520)
                     /locus_tag="ACHLAM_005591"
     CDS             complement(<1..>520)
                     /locus_tag="ACHLAM_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7856920.1"
                     /translation="QGRYRVKMDFDREETEPGFGYLWLRMAKPCAGETLGWHTPLIDG
                     TEVAIAYSNGDIDLPYIAYALHDAEHPDLVSRDNHTRNILRTPANNKLRMEDRRNEEH
                     IKLATEYGKTQLNSGHLVDSQGQLRGKGTELRTDEWGTLRAGKGLFVAADAQAKAQGE
                     ALDMDAALKEID"
BASE COUNT           80 a          172 c          132 g          136 t
ORIGIN      
        1 ctgtcgattt ccttcagcgc ggcatccatg tccagtgcct cgccctgtgc tttcgcctgc
       61 gcatccgcag caacaaaaag tcccttccct gctctcaggg ttccccactc atcggtacgc
      121 agctctgtgc ctttaccccg cagttgcccc tggctgtcca ccagatgtcc gctgttaagc
      181 tgtgtcttgc cgtattccgt agcgagtttg atgtgctcct cattgcgtct gtcttccatc
      241 cgcagcttgt tgttcgcagg cgttctgagg atgttccggg tgtgattgtc ccggctcacg
      301 aggtcaggat gctcagcgtc gtgcagtgca tacgcgatgt agggcaggtc aatatcgcca
      361 ttgctgtatg caatcgccac ttcggtgcca tcaatgagcg gcgtatgcca gcccagcgtt
      421 tcacctgcac agggttttgc catccgcagc cacaggtagc cgaatccggg ttccgtctcc
      481 tcccggtcaa agtccatctt cacccggtaa cgcccctgcc
//
LOCUS       DAZGAP010000579          518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZGAP010000579 DAZGAP010000000
VERSION     DAZGAP010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.583"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>518
                     /locus_tag="ACHLAM_005592"
     CDS             <1..>518
                     /locus_tag="ACHLAM_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO7856921.1"
                     /translation="QRKDRIWPDDKKSKLIESILMKLPLPGFYFGEKPNGNWVVIDGL
                     QRTTTICDYMSGHFSLKGLSILEHLNGKSFKDLTRTEQRDIREYQITAYQIELNDDSS
                     ELVVELFHRINTYGVKLSSQEIRSALNKGNSVTFLRYLASLETFKKATQFKVKPDRQK
                     DMELCLSALAFM"
BASE COUNT          182 a           81 c          103 g          152 t
ORIGIN      
        1 ttcagagaaa agaccgaatt tggcctgacg acaaaaagtc aaaacttatt gaatcaatat
       61 taatgaagct tccattaccg gggttttact ttggtgaaaa gccaaacggt aactgggtag
      121 ttattgatgg ccttcaaaga acgactacaa tttgtgatta tatgagtggt catttctcat
      181 tgaaagggct ctctatttta gagcacttaa atggaaagag ttttaaagat cttactcgaa
      241 ccgagcaacg agacattaga gagtatcaaa ttacggctta ccaaatagaa ctaaatgatg
      301 atagttctga attggttgta gaactattcc atagaataaa cacttatggt gtaaagttaa
      361 gtagtcaaga aataagatct gcacttaata aaggcaatag cgtaaccttt ttacgttatt
      421 tagcttcatt agaaacgttc aaaaaagcga cgcaatttaa agtaaagccg gacaggcaaa
      481 aagatatgga gctctgtttg tccgctcttg cttttatg
//
LOCUS       DAZGAP010000580          514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZGAP010000580 DAZGAP010000000
VERSION     DAZGAP010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.584"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>514
                     /locus_tag="ACHLAM_005593"
     CDS             <1..>514
                     /locus_tag="ACHLAM_005593"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7856922.1"
                     /translation="QEYIQRCSTGSTIKNVSLKMMRDFRFLMPESKEEQEKVVRIIQK
                     IDELKRLNNAQNQTLEQMFQALFKSWFVDFDPVIDNALDAGNPIPETLQSRAELRQNV
                     RNSTDFKPLPAEIRSLFPSEFEETELGWVPKGWESETFDSFCDLIQSGGTPSRKETSF
                     WDGGTIKWLSS"
BASE COUNT          168 a           92 c          117 g          137 t
ORIGIN      
        1 tcaagagtat atccaaaggt gttcaactgg gagtacaatt aaaaacgtat cactaaaaat
       61 gatgcgtgat ttcaggtttt taatgcctga atcaaaagag gagcaggaaa aggttgttcg
      121 tataatacaa aaaattgatg aactaaaaag attaaataat gctcaaaatc aaaccctgga
      181 acaaatgttc caagccctgt tcaaatcctg gtttgtagat tttgatccgg taattgataa
      241 cgctctggat gcaggaaatc caatcccgga aaccctgcaa tctcgtgccg agttgcgtca
      301 aaatgtacgt aatagtacag atttcaaacc gcttccggcg gaaattcgct cgcttttccc
      361 aagtgaattt gaagaaacgg agttgggttg ggtgccaaag ggttgggaaa gtgaaacttt
      421 cgattcattc tgcgacttaa ttcagagtgg gggaacgcct tcaaggaagg aaacttcatt
      481 ctgggatggc ggtacaataa aatggctttc atct
//
LOCUS       DAZGAP010000581          509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZGAP010000581 DAZGAP010000000
VERSION     DAZGAP010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.585"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>509
                     /locus_tag="ACHLAM_005594"
     CDS             <1..>509
                     /locus_tag="ACHLAM_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7856923.1"
                     /translation="KNIAEAVVSDEGATGGKALQLNKPESHIWFLEHDAAGQGAELLK
                     KGGRVSVRFKVPGALVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLN
                     AMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGA
                     ADKLQLTGI"
BASE COUNT          124 a          118 c          167 g          100 t
ORIGIN      
        1 ataaaaacat cgctgaggct gtggtcagtg atgagggtgc caccggtggt aaggccctgc
       61 aactgaacaa accggaaagc cacatctggt ttctggagca tgatgccgcc gggcaggggg
      121 cagagttgct gaagaaaggc ggacgtgtga gcgtgcggtt taaggtgccg ggcgcactgg
      181 tgccgaatca gtttgccctg ggtatttact ggcagttgtc gtccctgccg gagggagtga
      241 cgctgtcaga ggaaggcaac gacatgctga tgtccttctt cctgcagacg gatgcgacga
      301 acctgaacgc gatgtaccac aagaagcaga atgcgaagct ggatacgttc ggggtctttg
      361 ataacggatg gcacacactg gcttttgagt ttgccggaaa caacagcatt caggtgacgc
      421 cggtactgga tgagaaacgg ggtacaccgt tcacactggt gaaatctccg gcatcagggg
      481 cggcggacaa actgcaactg acaggcata
//
LOCUS       DAZGAP010000582          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZGAP010000582 DAZGAP010000000
VERSION     DAZGAP010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.586"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..227)
                     /locus_tag="ACHLAM_005595"
     CDS             complement(<1..227)
                     /locus_tag="ACHLAM_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856924.1"
                     /translation="MSSSVKYSPVPAVLTRPESLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTV"
BASE COUNT          133 a          146 c          129 g           98 t
ORIGIN      
        1 acggtcatca ctgtctccgg gcgggaaccg tcctgaaaaa catgtccggt gcgtggagaa
       61 ctgtatgaaa cataaccacc gtgcaccgca atgaccggac gacctgacgc cggaagcccc
      121 acacgacacc cactggcaat ccgggtcatc agcaggttca gttcagccag cgattcaggg
      181 cgggttaaga ccgccggaac cggagaatat ttcactgagg aagacatact ttcacctcca
      241 tatctctcag cccgccaggt gcgtcacttc cccgcagata accggaaacc tggctatatg
      301 aaaaaaggga aaagggtgcc ggacgaacag ctgtcgggaa taaaatcccg gtaactgtct
      361 gtacgttttc agaccggtaa gtttggcgac cctggtcaga aggtacggac agcatctttg
      421 cgctcataac tgtaccgacc gggatatgca ttacaaagac tgtcataccg ccccctccgt
      481 ctttcgctgt ttgcaggaaa gaccac
//
LOCUS       DAZGAP010000583          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZGAP010000583 DAZGAP010000000
VERSION     DAZGAP010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.587"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..390
                     /locus_tag="ACHLAM_005596"
     CDS             <1..390
                     /locus_tag="ACHLAM_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO7856925.1"
                     /translation="MKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSE
                     KQLTVTSSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGV
                     DLPNEAPGFSLTDITQESKISSKSFND"
BASE COUNT          141 a          111 c          141 g          113 t
ORIGIN      
        1 atgaaaaatc tgtcggtaac ggtggaaaaa gcccttggtc tgtttgtgca taaagacggg
       61 gcgaagctgg tagccaacca gggagatatt gagattcagg ctcagcacaa cacgatggcg
      121 ctgttctctg aaaaacagct gacggtgacc agtagtgaag acgaaattat tatcagcacc
      181 ccggaaactc tgacgctgaa cggtggtggg tcttacctga gactgagtaa gaacgggata
      241 gagcacggct catcgggtga gtttattgtg aagacgtctg actatctggt gcctggttct
      301 ggcgtagatc tccccaacga agcacctggt ttcagcctga cggatatcac acaggaaagc
      361 aaaatcagca gcaagtcttt taacgactga cagggagtat gaatcgtgtc tgtattaccc
      421 aaaatcgtct ggccgatccc ctccagccgt cggggaagtg agttcaaaaa tcaggaagag
      481 atcctctctc atttgggggg agaatc
//
LOCUS       DAZGAP010000584          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZGAP010000584 DAZGAP010000000
VERSION     DAZGAP010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.588"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..483
                     /locus_tag="ACHLAM_005597"
     rRNA            <1..483
                     /locus_tag="ACHLAM_005597"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          127 a          123 c          147 g          109 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 ttaccttaaa gaagcgtact ttgcag
//
LOCUS       DAZGAP010000585          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZGAP010000585 DAZGAP010000000
VERSION     DAZGAP010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.589"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(58..159)
                     /locus_tag="ACHLAM_005598"
                     /pseudo
     CDS             complement(58..159)
                     /locus_tag="ACHLAM_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(415..>506)
                     /locus_tag="ACHLAM_005599"
     CDS             complement(415..>506)
                     /locus_tag="ACHLAM_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO7856926.1"
                     /translation="TGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          143 a          103 c          105 g          155 t
ORIGIN      
        1 ttgtaagttc gcaggtattg tccgatcgtt gaatcatttg gacgttataa cccaaatttt
       61 taccgtccaa taatcaatat ttctgttgtg attgttaacc actgcgtgca ttggcgggat
      121 tgaagggtga aaaatatgtg caattgggaa gcctttcatt gatgcctgcc ttgtcatcat
      181 gacaaacaat aatagatgct tattttcctc gcagggttct ttcgcactat caaaaaatgc
      241 tcatttaccc ggcattacag tcaacgaagc aacattctat ggcctacgct gatgtaaagc
      301 acagtgagaa tcccccttgt tgtcttcgtc tgaccttatc gcggcgacca gcaattattc
      361 gttttgtgat cctcaacaca aaatgctatt cgtcgcctat gaggggagag gaatttatgg
      421 aaaacgattg cattcacaaa atgacgtcct gtcagaagag aaaaagttga cgttaatcaa
      481 ttgttcgaga tgatcgatac ctgtat
//
LOCUS       DAZGAP010000586          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZGAP010000586 DAZGAP010000000
VERSION     DAZGAP010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.590"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(22..441)
                     /locus_tag="ACHLAM_005600"
     CDS             complement(22..441)
                     /locus_tag="ACHLAM_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7856927.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
BASE COUNT          107 a          167 c          102 g          126 t
ORIGIN      
        1 gggtttccgt gctgatattc attacccgtt ctccccgtcc agcgcctgac gcattacccg
       61 cataatctca cgttcttccc tgacaaatgc gtcaccgtcg cccccgttct cgctccctac
      121 agcatggagg cgataaattt cactaccagc catcaccatc actttttcca gcagcaccgc
      181 agggactgtt acaagacggt ttacgcgctt ttcctccatc agttcattga caatatcgga
      241 aacaactctg tcacatgcgc catatacaaa agcatcgtcc agccgctgca tgacggtttc
      301 cagctcatcg tctgacaggt tccagtcccg ggctatgtgg cgcacatccc cgcgggtcag
      361 caccacggca aaaacacatt cactgtccgg cacgtctgta ttcagcactt cgcggataat
      421 ttctttaaca gtacagtaca tgttctcgtc tcctttacag gggcggcatt gccgccccgt
      481 ccgttaatca gtccagtgcc gc
//
LOCUS       DAZGAP010000587          501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZGAP010000587 DAZGAP010000000
VERSION     DAZGAP010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.591"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>501
                     /locus_tag="ACHLAM_005601"
     CDS             <1..>501
                     /locus_tag="ACHLAM_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO7856928.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRL
                     TTIQNDRTRIQTVYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDH
                     RGLPLAL"
BASE COUNT          104 a          149 c          173 g           75 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtatggcg gcgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccggaag tgacctggta cggctgggac
      121 ggcgaccggc tgaccacgat acagaacgac agaacccgca tccagacggt ttatcagccg
      181 gggagcttca cgccactcat cagggtcgaa accgccaccg gagagctggc gaaaacgcag
      241 cgccgcagcc tggcggatgc ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatacaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct t
//
LOCUS       DAZGAP010000588          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZGAP010000588 DAZGAP010000000
VERSION     DAZGAP010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.592"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..334
                     /locus_tag="ACHLAM_005602"
     CDS             <1..334
                     /locus_tag="ACHLAM_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856929.1"
                     /translation="AGRLKRYYKFYSNEEVDAIRNNGHANGKWAAAAISQNGHMWDAM
                     PGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYITYASPAPGHLKDKFIEPLVKRVK
                     DVAEGRVR"
BASE COUNT          151 a           83 c          151 g          109 t
ORIGIN      
        1 tgcgggaaga ctgaaacggt actataagtt ttattcaaat gaagaagtcg atgcgattcg
       61 taataacgga catgcgaacg ggaagtgggc cgcagcagca ataagtcaga acgggcatat
      121 gtgggatgcc atgcccggta gttattataa aatgggcagt gactgggatg gtggcgaatt
      181 tgatgatcat ctgacgcttg aacttgaaaa aaatggcaaa ggaagtcgga tatatataac
      241 gtatgcatct ccggcaccgg ggcatctgaa agataaattt attgagccat tagttaaacg
      301 tgtgaaggat gtggctgaag gccgggtcag ataaaattaa cccgctggta ttgtcgttgt
      361 gatgggctcc tctggagggg tgattatgct cagaggggct gaaagtaagg gacgcagagg
      421 gcgacgacca gcgacgtctg gaagagggcg tgatatgtaa ggaagcacac cctgacggca
      481 tatcataaaa aaac
//
LOCUS       DAZGAP010000589          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZGAP010000589 DAZGAP010000000
VERSION     DAZGAP010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.593"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>492
                     /locus_tag="ACHLAM_005603"
     CDS             <1..>492
                     /locus_tag="ACHLAM_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO7856930.1"
                     /translation="LKDSTGKEKEVGSKNGGTTGNEVTVTGKGDIDVTVDGNGLSIDG
                     TKLAENIKKDAIKIKTGDGKTLTKKLGEEVEFNDAGPIKTKINNNKLEITAETASFTP
                     DGNGGNGKITVSNGDDDKLVTAKNIADAINQSGWKIKAGSENGGKVEGDNKEELINPG
                     DTVT"
BASE COUNT          191 a           66 c          130 g          105 t
ORIGIN      
        1 gttaaaagac agcacaggaa aagaaaaaga ggtcggcagt aaaaatggcg ggactacagg
       61 taatgaagtg accgtcaccg gtaaagggga tattgatgtt actgttgatg gaaatgggct
      121 gagtattgat ggcacaaagc ttgctgaaaa catcaaaaaa gatgccatca agattaaaac
      181 gggagatggt aaaacactaa cgaaaaaact tggtgaagaa gttgagttta atgatgccgg
      241 accaataaaa acaaaaatta ataataacaa acttgagata actgcagaga cagccagttt
      301 tacgcctgat ggaaatggtg gtaacgggaa gattacagta agcaatggtg atgacgataa
      361 gcttgttact gcgaaaaata ttgctgatgc cataaaccag tctggctgga aaattaaagc
      421 tggcagtgaa aatggtggga aagttgaggg ggataataaa gaggagctaa ttaatcccgg
      481 tgatacagtt ac
//
LOCUS       DAZGAP010000590          488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZGAP010000590 DAZGAP010000000
VERSION     DAZGAP010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.594"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(143..334)
                     /gene="yfgG"
                     /locus_tag="ACHLAM_005604"
     CDS             complement(143..334)
                     /gene="yfgG"
                     /locus_tag="ACHLAM_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO7856931.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            352..>488
                     /locus_tag="ACHLAM_005605"
     CDS             352..>488
                     /locus_tag="ACHLAM_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856932.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     YV"
BASE COUNT          153 a           86 c          120 g          129 t
ORIGIN      
        1 cagtcgaaca ggataaacat cagtcaggac aaaaacgtaa aatttaagat gaaaaaatat
       61 ctgccgcttt gataatccca aagagcaaaa gcatccgaca gcgaggtgcc gttatgttct
      121 gtgagagtgg agaaggtaac cgctaacgtt gtactggtga ttcgacggag agtgtggatt
      181 gctgagcttc ttttttgctc tgatggtgct gccaggcacc gacggacgag tagataaaac
      241 ggccaaagaa gaagataaag ctgatgagca gtacgatacg ggtcatgcga ctgttaaatc
      301 ggtgtcgttt tcgcatactg gttgcctgac tcacaaaagg ttccttgaag tatgtcccac
      361 gccgtggacg gtacttacat taaggcacaa caggacaaaa tggtcaattc ttcgttatgt
      421 aaaaaagcgt cagtggatac atattttaat gttatggaag ttaatttaat tatttacaat
      481 gattatgt
//
LOCUS       DAZGAP010000591          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZGAP010000591 DAZGAP010000000
VERSION     DAZGAP010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.595"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..481)
                     /locus_tag="ACHLAM_005606"
     CDS             complement(<1..481)
                     /locus_tag="ACHLAM_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856933.1"
                     /translation="MNINKLLLDVDNPRFPTSAENQRDAIAKMLDLQYDRIYRLAKDI
                     ASKGLDPSENILVYPSEEESGFFVVAEGNRRVTALKLLHSPRLAPTEKARKAFEKLKI
                     TQLKDINVIDNCVLFDDDEYEHWVNLKHTGQNAGVGRVEWTATEKARHLARMGKQSFG
                     "
BASE COUNT          143 a          106 c           84 g          150 t
ORIGIN      
        1 taccaaatga ctgcttaccc atcctagcca gatgtcgtgc tttttctgtt gcagtccatt
       61 caactcttcc aactccagca ttttgaccag tatgctttaa attgacccaa tgctcatatt
      121 catcatcgtc aaaaagaacg cagttatcta taacattaat atctttaagc tgagtaatct
      181 taagtttttc aaaagctttc cgtgcttttt ctgttggtgc aagtctagga gagtgtaaaa
      241 gtttaagggc tgtaactcga cgattacctt cagcaacgac gaaaaaccca gattcctctt
      301 cactaggata aactaatatg ttttcagacg gatctaaacc tttcgaagca atatctttag
      361 ccaagcgata aatgcggtcg tactgcaagt caagcatctt ggcaatagca tcacgctgat
      421 tttctgctga cgtcgggaaa cgagggttat caacatccaa aagtaactta ttgatattca
      481 ttg
//
LOCUS       DAZGAP010000592          481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZGAP010000592 DAZGAP010000000
VERSION     DAZGAP010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.596"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..324
                     /locus_tag="ACHLAM_005607"
     CDS             <1..324
                     /locus_tag="ACHLAM_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856934.1"
                     /translation="MDYQDNIALEQVVTWDSDNIFTPNIFEHNGRCFMKKNETWMLLN
                     GDATPNIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSKPAKSVNTGKR
                     PALGY"
BASE COUNT          180 a           90 c          108 g          103 t
ORIGIN      
        1 atggactatc aagataatat agccctcgag caagtcgtaa catgggattc tgataatata
       61 ttcacaccta atatctttga acataatgga agatgtttta tgaagaaaaa tgaaacatgg
      121 atgttattaa atggagatgc aacaccaaac attagaacaa tgagcaataa gaaaatagaa
      181 tgcctggaga agagtgaagt agaatgggct agtagggttt gcgacagtat cacagacaag
      241 gaaagggaaa atctgaaggc attaatatgt agcaaaccag ctaaatcagt taatacaggc
      301 aaaagaccag cgttaggata ttgaaaccgg cactgcactc tatcactatt acaatattct
      361 gagtgactag agcaactgct ccgccatccg ggctagcagt atgacgacga atccgaactg
      421 cactacaact ggcaccggta ctacaatccg ggacagggac tagggaaaga tcacgcaacg
      481 c
//
LOCUS       DAZGAP010000593          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZGAP010000593 DAZGAP010000000
VERSION     DAZGAP010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.598"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..464
                     /gene="ydeI"
                     /locus_tag="ACHLAM_005608"
     CDS             72..464
                     /gene="ydeI"
                     /locus_tag="ACHLAM_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO7856935.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          147 a           96 c          113 g          123 t
ORIGIN      
        1 ttcttatttg tcaattataa aaatagcaac cagacttaat aagtcaaacc aaacgcaatt
       61 aaggagtcag tatgaaattt caggccattg tattagcaag ttttcttgtc atgccttatg
      121 cgttggcaga tgaccagggc ggattaaaac aggatgcagc gccaccgccg cctcatgcaa
      181 tagaagatgg ctatcgcgga accgatgatg caaaaaaaat gaccgttgat ttcgcaaaaa
      241 acatgcacga tggcgcttcg gtttcactac gcggtaattt gatttctcac aaaggagagg
      301 accgttacgt ttttcgcgat aagagtggtg aaattaatgt tgttattcct gcggcagtct
      361 ttgatggacg agaagttcag ccggaccaga tgatcaacat tagcggcagt ctggataaga
      421 aaagcgcgcc ggccgtcgtt cgggtcactc atttgcagaa ataaaaataa attagcctg
//
LOCUS       DAZGAP010000594          477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZGAP010000594 DAZGAP010000000
VERSION     DAZGAP010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.599"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            119..>477
                     /locus_tag="ACHLAM_005609"
     CDS             119..>477
                     /locus_tag="ACHLAM_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856936.1"
                     /translation="MKKYILVSLMGLSLNAFAGGSGWNPDRVDPSRCVMATGSGPTSA
                     GYMVYRTTDSVCMQGINEGKVKGVHIYDGHHEFNDGTVDTFSGNVTPISPLTLKTDVQ
                     KVNKVGIKRWWYKASWI"
BASE COUNT          146 a           73 c          124 g          134 t
ORIGIN      
        1 gtagtgtata agtgagttcc caagagcttg cataagtaag tgactgaggg agcgtaagtg
       61 cagtacatgc aacttgttgc atgacaaata tattatctat taacaataag aggttataat
      121 gaagaaatat attttagtat ctctgatggg attaagtttg aacgcatttg cgggtggcag
      181 cggctggaac ccagacaggg tagacccgag ccgttgtgtt atggcgacgg ggtcaggtcc
      241 tacctctgcg ggttatatgg tataccgaac gactgactca gtgtgtatgc agggtataaa
      301 tgaaggaaaa gtgaaaggtg tccatattta tgatgggcat catgagttca atgatggtac
      361 tgttgacacg tttagcggta atgttacccc aatatcacct ttgacattaa aaactgatgt
      421 tcaaaaagtt aataaagttg gcatcaaacg ctggtggtat aaagctagct ggattaa
//
LOCUS       DAZGAP010000595          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZGAP010000595 DAZGAP010000000
VERSION     DAZGAP010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.600"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHLAM_005610"
     CDS             complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHLAM_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO7856937.1"
                     /translation="TKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTK
                     LENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNERTTA
                     "
BASE COUNT          112 a          128 c          108 g          125 t
ORIGIN      
        1 ttcactcact gtcagaagac cttcagacac gctaccgccc ttcgccataa cccgggcgca
       61 tcaggtctgt gttcaggatt gagaattctt tcttcagcac ccgccaacag atactgaaga
      121 aaaaagaaaa atgacggaca ggataccctg cccgtccggg agaattacgc agtggtacgt
      181 tcattccacg cgtcagaatg aatgatgtta ccgtccttat aagtccaggt gattttctcg
      241 tagcgcagct ccacctgctc aaggtggtta tgcttctcgt aggcgggatt cttgatgtcg
      301 tgcatgaccg ggttaacctt caccaccttc acgttctcaa gcttcgtatt gaaatactcc
      361 acttcctgac cggcatcatt gattctgtac cacttaaatt cagcagactt cagggtctgc
      421 cccgtggtca ccgccttgta aaggtagggg ctggaggaat caatttcctt ggt
//
LOCUS       DAZGAP010000596          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZGAP010000596 DAZGAP010000000
VERSION     DAZGAP010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.601"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>464
                     /locus_tag="ACHLAM_005611"
     CDS             <1..>464
                     /locus_tag="ACHLAM_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFO7856938.1"
                     /translation="WKQRHAEGYASVYGGYKGKESQNIFFVPLMTDENGANVQTNDPA
                     EDPDLEAVGYYGSKWRNDQKTWTSVDRASHFSSWARRGIISDRLATAILVHAGRTAEF
                     ITGKQPDTVKPTGPSGEGPEREPEAPVSNRTLMSLLASGEDLASQGWRYYHK"
BASE COUNT          116 a          112 c          144 g           92 t
ORIGIN      
        1 tctggaagca gcgtcatgcg gaagggtatg catcagtata cggagggtat aagggtaagg
       61 agtcgcagaa cattttcttt gtaccgttaa tgacggatga gaatggtgcg aatgtgcaga
      121 ccaatgaccc ggcagaagac ccggacctgg aagccgtcgg ttattacggt tcaaagtggc
      181 gtaatgacca gaagacctgg acatctgtgg acagggccag ccatttcagt tcatgggctc
      241 gccgtgggat tatttccgac cgtctggcaa cggcgattct ggtgcatgcc gggagaaccg
      301 ctgaattcat taccggaaaa cagcctgata cggtgaagcc caccggacct tccggtgaag
      361 gcccggagag agagccggaa gccccggtca gtaaccgaac cctgatgagt ctgctggcgt
      421 ccggtgaaga cctggcatca cagggctggc gctattatca caaa
//
LOCUS       DAZGAP010000597          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZGAP010000597 DAZGAP010000000
VERSION     DAZGAP010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.602"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..235)
                     /locus_tag="ACHLAM_005612"
     CDS             complement(<1..235)
                     /locus_tag="ACHLAM_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856939.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFSTADIDDGFKY"
     gene            complement(228..>455)
                     /locus_tag="ACHLAM_005613"
     CDS             complement(228..>455)
                     /locus_tag="ACHLAM_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7856940.1"
                     /translation="MGHKVMDKYGSESWTHKGTSTRGQSTIPNTPMPSSGEIDIMKYA
                     DSYAPDRYKRLVAADEDVQALIWLSRVSFND"
BASE COUNT          151 a          105 c           63 g          136 t
ORIGIN      
        1 catatttaaa accatcatca atatctgccg tggaaaaata tactaataca tcactatcac
       61 caactggcaa gtgtatatta tgctgataaa tcagcgtaga accggaaaca tatccctgaa
      121 taaagacatc gatatctgag gagcatctat tctcaatcct gactatattg gaatactctt
      181 tttgtgaagt acacccactc aaaaaaaaca ataaaaaaac aattattcta atcattaaaa
      241 ctcaccctgc tcaaccagat caatgcctga acatcttcat ctgccgcgac caatctctta
      301 tatctatcag gtgcatagct atctgcatat ttcattatat caatctctcc tgaagatggc
      361 attggagtat tagggatggt actttgaccc cgcgtggatg tccccttatg tgtccatgat
      421 tcagaaccat atttatccat aactttatgt cccat
//
LOCUS       DAZGAP010000598          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZGAP010000598 DAZGAP010000000
VERSION     DAZGAP010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.603"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(22..249)
                     /gene="mchB"
                     /locus_tag="ACHLAM_005614"
     CDS             complement(22..249)
                     /gene="mchB"
                     /locus_tag="ACHLAM_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO7856941.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALARIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(277..>453)
                     /gene="mchI"
                     /locus_tag="ACHLAM_005615"
     CDS             complement(277..>453)
                     /gene="mchI"
                     /locus_tag="ACHLAM_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO7856942.1"
                     /translation="IFSLPITILLVSLSSLRIVGEGNSYVDVFLSFIIFLGFIELIHG
                     IRRILVWSGWKNGS"
BASE COUNT          157 a          117 c           76 g          103 t
ORIGIN      
        1 gtaaaataaa taaaaatata attagctacc gccaccagca gaagaactgg cactaccact
       61 tcccacggtc gagccaattg ctgttgtcaa accggcagat acggctccaa ctacaacccc
      121 aagtggacca ccaggtattc tggcgagagc tccaacaata gctgcagcac ctgcagcatt
      181 agctgaagtc gctggcgcac ctcccgcccc ggaaatatat cttaactgtg attctgttat
      241 ttctcgcata taaactgcat ataaactcca tcatatttaa cttccgtttt tccagcctga
      301 ccagaccaga atccttcgaa tcccatgaat cagctcaata aaaccaagaa atattataaa
      361 gcttagaaaa acgtcaacat aagaattccc ttcgccaaca atccgaaggg atgaaagtga
      421 aaccaataag atagtaatag gtaaactgaa tat
//
LOCUS       DAZGAP010000599          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZGAP010000599 DAZGAP010000000
VERSION     DAZGAP010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.604"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..340)
                     /locus_tag="ACHLAM_005616"
     CDS             complement(<1..340)
                     /locus_tag="ACHLAM_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO7856943.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNV"
BASE COUNT          120 a          113 c          115 g          102 t
ORIGIN      
        1 gcacattacg cagacgaagg cgaccatccg gcgcatgaag catggccgtt ttcagttgct
       61 catgggggtc gttggagacg atgaggtcaa gccccctgac agccgtacgg gaaatgatgt
      121 tgtcaggatg actgaggtca gccgtgcgca tcagtaattc aaaaagaccg gccggcgctt
      181 catacgtcag cggaaagata ttgcttgccg tgggctgtgc atggtctccg tctatcagca
      241 gagtacggag gccggcatct gccagaaaac cggcaagatt agcagactgg gttgattttc
      301 cttcgccacc tttagtggat ataaccggta aaattttcat atagcacact ctttaaagta
      361 tgcaaccaat cacttacact acggaaacca gccagaatat tcacttcact acctgaatac
      421 tcgcaacacg gggattgaaa atccccgtgt
//
LOCUS       DAZGAP010000600          447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZGAP010000600 DAZGAP010000000
VERSION     DAZGAP010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.606"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>447)
                     /locus_tag="ACHLAM_005617"
     CDS             complement(<1..>447)
                     /locus_tag="ACHLAM_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7856944.1"
                     /translation="MFPDAVLSLLAPFASTVQGGQRTRRLILLIWLCVLTALGISALN
                     NRDLIRQVSTDLQRWNAVPKGHYRPEAESLAALKQDALLLEQWQRQGVPLRYSLGYYP
                     GQRLWLALQQAIDTRVPPPSPEPEPVPEIVRLDSLALFDAGKWTLK"
BASE COUNT          104 a          135 c          124 g           84 t
ORIGIN      
        1 gtttcagcgt ccatttaccg gcatcaaaca gtgccaggct atcgagtcgt acaatttccg
       61 gcaccggttc tggttcaggg gatggtggtg gaacccgggt atcgatagcc tgctgcaggg
      121 ccagccacag acgttgtccc gggtaatagc ccaggctgta acgtaaaggc actccctgac
      181 gctgccactg ctcaagcaga agcgcatcct gtttcagtgc ggccagcgat tccgcctcag
      241 gacggtagtg ccccttcggg acagcattcc agcgctgtaa atcggtgctt acctggcgga
      301 tcagatcacg attgttaagc gcagaaatcc ccagcgcagt caggacacac agccatatca
      361 gcaaaatcag acgacgggta cgctggcctc cctgtaccgt cgacgcaaaa ggtgccagca
      421 gcgacagaac cgcatccggg aacatcc
//
LOCUS       DAZGAP010000601          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZGAP010000601 DAZGAP010000000
VERSION     DAZGAP010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.607"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          153 a          118 c           69 g          105 t
ORIGIN      
        1 tataaaagca taaaaattat attctttaag caataagcat ctaaaaacaa aaagctaccg
       61 caaggagaat ccctcctaca aaaaaacaac attagaaaca gtaatacatt agctatgtat
      121 tcttatgcac acctagatag gttatattat aacattcaga tgaatcaccc tgcgacaaac
      181 ttctcatcaa agaaatatca aacaggatgc agcaaagtaa aacccaccta ttaataaaaa
      241 agccccggac attccggggc tacactgcat attaacgaac gctctgtccg gttatttcaa
      301 ccctgcgatt ggggcgcaga cattccttca gtgcttcgcc ctccacatcc gggcagaaca
      361 ccactggtgc atcatatcca cggcccgttg catgtatcct ttcagccctg aatccccggg
      421 caataaacca tcccctgact gattc
//
LOCUS       DAZGAP010000602          439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZGAP010000602 DAZGAP010000000
VERSION     DAZGAP010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.608"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>439
                     /locus_tag="ACHLAM_005618"
     CDS             <1..>439
                     /locus_tag="ACHLAM_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7856945.1"
                     /translation="TSAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSD
                     SGRLTGVHTTTSNLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRY
                     DRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSRHRLVHYTRTQ"
BASE COUNT          107 a          155 c          114 g           63 t
ORIGIN      
        1 atacctctgc cgggcagtta cagagccagc acctgaacag cctgcagtat gaccgcgatt
       61 acacctggaa cgacaacggc gaactcatcc gcatcagcag cccgcgacag acccggagtt
      121 acagctacag cgactccggc aggctgaccg gcgtccacac caccacctca aatctggata
      181 tccgcatccc gtatgccacg gacccggcag gtaaccgcct gcccgacccg gagctgcacc
      241 cggacagcac cctcagcatg tggccggata accgtatcgc ccgtgacgcg cactatcttt
      301 accggtatga ccgtcacggc aggctgacag agaaaaccga cctcatcccg gaaggggtta
      361 tccgcacgga tgatgagcgg actcaccggt accattacga cagccggcac cggctggtgc
      421 actacacgcg gacacaata
//
LOCUS       DAZGAP010000603          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZGAP010000603 DAZGAP010000000
VERSION     DAZGAP010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.609"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>437)
                     /locus_tag="ACHLAM_005619"
     CDS             complement(<1..>437)
                     /locus_tag="ACHLAM_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO7856946.1"
                     /translation="VITGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKI
                     TGLLTAEKARGMKGDEINAWRNAMEAANYMFEHNGRKWDYGKSTQTRLEPSVAAAKAG
                     KLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVR"
BASE COUNT           73 a          162 c           88 g          114 t
ORIGIN      
        1 cggacgtgga tttccatccc tttggtgaac atcgcctgct ctgccgcttc actcagtgct
       61 ataagctctt ctgctgtcac ctccacatca ttgttttccg catccgtcca gaaaaacgcc
      121 tccggcagtt tccccgcttt cgctgccgcc accgacggct caagacgcgt ctgcgttgac
      181 ttcccgtagt cccatttacg cccattgtgc tcaaacatgt agttcgccgc ttccatcgca
      241 ttacgccagg cgttaatttc atcgcccttc atccctcgcg ctttctccgc ggtcagcagg
      301 cccgtgattt tctccccgtc aaaaccccag cgccccgaga tgtcagcttt gcggttttct
      361 tccgtatccg ccacctccac cacactctgg cccactggcc acagaactga tgccaccctg
      421 ctgatgccgg taatgac
//
LOCUS       DAZGAP010000604          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZGAP010000604 DAZGAP010000000
VERSION     DAZGAP010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.610"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>437
                     /locus_tag="ACHLAM_005620"
     CDS             <1..>437
                     /locus_tag="ACHLAM_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7856947.1"
                     /translation="LHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQD
                     FEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASA
                     HMPFIGSVGPAFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFR"
BASE COUNT           93 a           93 c          132 g          119 t
ORIGIN      
        1 tgcttcatca tccggaattt cagaaagtgg agtccctgtg gcggggcgtg aagtctctgg
       61 tggataaaac ggacttccgc cgtaatgtga aaattgaact gctggatctg tccaaagacg
      121 atctgcgtca ggattttgag gatgcgccgg aaattattca gagtggtctt tatctgcaga
      181 cttatgtggc ggagtacgat acgccgggtg gtgaacccat tgcagccctg atttctgcct
      241 gggagtttga tgcgtctgcg caggatgtgg cgctgctgaa aaatatttcc cgggtggctg
      301 cgtccgcgca tatgccattt attggttcag ttggcccggc tttcttccgg aaggatacga
      361 tggaagaggt ggccgccatt aaggatattg gcaatcactt tgagcgtgcc gagtacatca
      421 agtggaatgc tttccgt
//
LOCUS       DAZGAP010000605          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZGAP010000605 DAZGAP010000000
VERSION     DAZGAP010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.611"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          149 a           85 c           92 g          103 t
ORIGIN      
        1 cggtgcgctt acggataatt aatgtcactc tgtatggatg aaaaaactga cagcagaagt
       61 ctccgctgac aaagacagga atgatgatta atgtcaactt ggccagtgag ccagcccctt
      121 aaaacgccag acagtggatg tatattaaaa taagagatag gctgggaaaa tataaagagt
      181 aactgcgctt tctgagtaaa aaaacaacag cagcaggtac ttcaaaaccg tgatgcactg
      241 tcagttgcaa agacgaatgt acagttacct ttgttaccag aggacaagaa gtgccccggg
      301 tattaccgtg aaagaaatca gtgaacattt attgaccata accacattgc ttctccgtag
      361 actgttgatt tgaaagagtt aacatccccg taaaataatc aggcctgtat gaacctgatt
      421 ataccacat
//
LOCUS       DAZGAP010000606          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZGAP010000606 DAZGAP010000000
VERSION     DAZGAP010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.612"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            57..260
                     /locus_tag="ACHLAM_005621"
     CDS             57..260
                     /locus_tag="ACHLAM_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFO7856948.1"
                     /translation="MPHILLPELIPEAAKIVPVFEGEKQKGTIVVSTEDVFDGNNKEH
                     IGKANDIEIRLLDLGLLPLLTEL"
BASE COUNT          139 a           76 c           88 g          126 t
ORIGIN      
        1 ttcggatatg attgattcct tgcttctgtt ggctgaatgt gggatggatg ttatatatgc
       61 cccacatact cttacctgaa ttaatccctg aagccgcaaa aatagtaccg gttttcgagg
      121 gcgagaagca aaagggaact attgttgttt caaccgaaga cgtatttgac ggaaataata
      181 aggaacacat cggaaaagct aatgatattg aaataagact tctggatctt ggactgctac
      241 ctttgctaac agagctttaa tccaacctta tcactcatgg gtagatgacg ggacagttac
      301 acccgttaat ggccttattt ttaacacatc agataaaatt gaatgctcac cattaagaat
      361 acctggagaa attaaaaaac ggagattgtt tcaagagctt cttttattta agggtttaca
      421 ggtcaaaaa
//
LOCUS       DAZGAP010000607          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZGAP010000607 DAZGAP010000000
VERSION     DAZGAP010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.613"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..354)
                     /locus_tag="ACHLAM_005622"
     CDS             complement(<1..354)
                     /locus_tag="ACHLAM_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856949.1"
                     /translation="MLIYPAADLRLQGRRAQPWDKTTTHKYRPGQYYDFRKHPELIET
                     HLEDFVEYSDRQAIQTFFSFVKWINSNSSAFESTDCMFSGTPKVDEYAPVFGCTHASS
                     GRFEFLFRDTKINQNE"
BASE COUNT          116 a           92 c          101 g          119 t
ORIGIN      
        1 ctcattttgg tttattttcg tatcgcgaaa caaaaattcg aagcggccag atgacgcatg
       61 ggtgcaacca aataccgggg cgtattcatc aacctttggt gttcctgaaa acatgcagtc
      121 cgtgctctcg aatgcagatg aattgctgtt gatccactta acaaaagaaa aaaaggtctg
      181 aatcgcttgt ctgtctgaat attcaacaaa atcctcaagg tgtgtctcga tcagttccgg
      241 gtgtttacga aagtcataat attgaccggg acgatattta tgagtggttg ttttatccca
      301 tggttgtgct ctgcgtccct gtaaacggag gtctgccgct ggatagataa gcataagtaa
      361 tctcctggtg gaatgtaatc cacgatcatc cggggaaatt aagaacccgc cagtgcccac
      421 cactggtg
//
LOCUS       DAZGAP010000608          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZGAP010000608 DAZGAP010000000
VERSION     DAZGAP010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.614"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..280
                     /locus_tag="ACHLAM_005623"
     CDS             <1..280
                     /locus_tag="ACHLAM_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO7856950.1"
                     /translation="KKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMAR
                     HGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            277..>427
                     /locus_tag="ACHLAM_005624"
     CDS             277..>427
                     /locus_tag="ACHLAM_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856951.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGV"
BASE COUNT          112 a           81 c          137 g           97 t
ORIGIN      
        1 gaaaaaaaat ctcattgcat gggcgtggtc gagtggtctt attgagtttg gttacgtcct
       61 gccggaaggt gcattgccga tagttgccgg aaagcctgcc acggtacggc atgtgattga
      121 ggttatggcg cgtcatggac gtgatgaaca ggagcagtta ctggttccgg ggataccgga
      181 agcggtgacg gaggaagaag cctttaatgc catgattcgg ttctgccgtg aggtcagacg
      241 ccgggtcagt tatccaaaca gaacgaggac cagaggatga gtaaagtcgt acgcattatt
      301 ttcgaataca aggagcacgt tatccataaa aacgctgatg gaacagtgcg catgggggta
      361 agtctggaca tacgttcaac cgggataaag cagaaaggtg atggacccgc catgattttt
      421 ggggtgg
//
LOCUS       DAZGAP010000609          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZGAP010000609 DAZGAP010000000
VERSION     DAZGAP010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.615"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>425)
                     /locus_tag="ACHLAM_005625"
     CDS             complement(<1..>425)
                     /locus_tag="ACHLAM_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856952.1"
                     /translation="TSSGEPPTFSLTPDGKLTARNADISGNINANSGTLNNVVIAENC
                     TIKGTLRAENIIGDVVKTHNVSLPALSAAGDHRHATERTVTVIDDQSFDRNIVIPPVF
                     YSGIVYQGSDGDGATSICTLTVSVNDRVVFSKSSSIGTGR"
BASE COUNT          101 a          118 c           97 g          109 t
ORIGIN      
        1 cgcccggtcc cgatggatga gcttttgctg aacactaccc gatcattcac acttaccgtc
       61 agcgtacaga ttgaagttgc accatcacca tcacttccct ggtagacaat accggaataa
      121 aaaaccggcg ggataacaat gttccggtca aacgactgat catcaatgac agtaaccgtt
      181 cgttctgtcg cgtgtctgtg atcccctgct gcactgagag caggaagact gacattgtgc
      241 gttttcacca catcgccaat gatattttcc gctctcagag tacctttgat ggtacagttc
      301 tccgctatca cgacattatt gagcgtgccc gagttcgcat taatattccc gctgatatcc
      361 gcattacggg ctgtcagttt cccgtccggc gtcagggaaa acgtcggagg ctcacctgat
      421 gaggt
//
LOCUS       DAZGAP010000610          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZGAP010000610 DAZGAP010000000
VERSION     DAZGAP010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.616"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>415)
                     /locus_tag="ACHLAM_005626"
     CDS             complement(<1..>415)
                     /locus_tag="ACHLAM_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856953.1"
                     /translation="LEQVSTIPEKQWHAFVLAVTETFWQLPEALRPQNAYFGSLTRAS
                     ELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNFGRQPGDFFARTVFVLL
                     HNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFS"
BASE COUNT          116 a          122 c          102 g           75 t
ORIGIN      
        1 agaaaaattc tcatgcccca ggtaccgttt tatccatttc agcggcagcg accagctggc
       61 accaccgaca gtggaatgaa tacgaaaaca gtccggacac agattgtgca acagcacaaa
      121 gactgtacgg gcaaagaaat ctcccggctg acgtccgaaa ttcagctcat taagagccag
      181 gatattccgg ctgtgctccc gctcaatagt gacattcacc gaccacagcc cgtcggcaga
      241 ccggcagtaa aacgccagcg tgtccgttac cggaaacaac tctgacgccc gggtcaggga
      301 gccgaaatac gcattctgcg gccgtaacgc ctccggcaac tgccagaagg tttcagtgac
      361 agccagaaca aaagcatgcc actgcttttc cggtattgtg gaaacctgct caagg
//
LOCUS       DAZGAP010000611          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZGAP010000611 DAZGAP010000000
VERSION     DAZGAP010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.617"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>414
                     /locus_tag="ACHLAM_005627"
     CDS             <1..>414
                     /locus_tag="ACHLAM_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7856954.1"
                     /translation="ISKEFLMRKDGIDDVYQFIEKLKKEKVPLDKAEQQLKKFYSSNG
                     YDRGITLYNGLNEDYRPWGKINMSWPNADSFGPTYEVLHPLTNNPVKIPDRGWRWKEG
                     TFNHIAKRIDGKYADIKKLHDGSVICGGIWFARTE"
BASE COUNT          151 a           59 c           92 g          112 t
ORIGIN      
        1 aaataagtaa agaattttta atgcggaaag atggtattga tgatgtatat cagtttattg
       61 aaaagttaaa aaaggaaaag gtgccattag ataaagcaga gcagcaactc aaaaaattct
      121 acagctccaa cggatatgat cgtggaatta ctctttacaa cggtttgaac gaggattata
      181 ggccatgggg taaaattaat atgagttggc ctaatgcaga ttcatttgga cccacgtatg
      241 aagtattgca ccctttgact aataaccctg tcaaaatccc agacagaggc tggcgttgga
      301 aagaaggcac ttttaatcat attgcaaaaa gaatagatgg aaagtatgca gatatcaaaa
      361 aactacatga tggtagcgtt atttgtggtg gaatttggtt tgcccgtaca gaaa
//
LOCUS       DAZGAP010000612          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZGAP010000612 DAZGAP010000000
VERSION     DAZGAP010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.618"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(163..372)
                     /gene="fumD"
                     /locus_tag="ACHLAM_005628"
     CDS             complement(163..372)
                     /gene="fumD"
                     /locus_tag="ACHLAM_005628"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO7856955.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          116 a           99 c           66 g          132 t
ORIGIN      
        1 atttgtgaag ttcatcgcag aaaataaaaa taactttatc gctataaatt aaaatacaca
       61 ccgaaattcg tataatttca gcagttgtta tttagaatga ttaactggac cgcaactgaa
      121 gcaagaatat caggatgaag cttaccgcct catcctgaat ggtcatttta ccagtgcgtt
      181 aatgacggtt tccatcgcct gcttttccag ctcactgcgc tgaatgcgtt tattggctaa
      241 tgctgtacgt aatacgcctg cgataacaat atgctttggt tcctgcccca gatcgcgcat
      301 ttccagcaca actttaccga caactctgca catttctcgg tacagttcat cctcttttgt
      361 ccgatttccc attctttata cctatttatc atgccaacta tcagcatata tca
//
LOCUS       DAZGAP010000613          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZGAP010000613 DAZGAP010000000
VERSION     DAZGAP010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.619"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>407
                     /locus_tag="ACHLAM_005629"
     CDS             <1..>407
                     /locus_tag="ACHLAM_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7856956.1"
                     /translation="PDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRMEDKRQ
                     EEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADAQARA
                     QGPVLEMAAAISQINQANSQMQALNSAAEAAGAL"
BASE COUNT          106 a          112 c          127 g           62 t
ORIGIN      
        1 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
       61 agtgacaatc atacgcggaa cgtgtggcgc acgccggcga acaataagct gcggatggag
      121 gataagcgcc aggaagagca tatcaagctc gcaacggaat atggcaaaac acagctgaac
      181 atgggacacc tggtcaacag ccggcgtgaa aagcgaggtg ccggttttga actgcgaacg
      241 gatgagcatg gggctgtacg tgcagcaaaa gggctgttcc tgacggcaga tgcccaggcc
      301 agagcccagg ggccggtgct ggaaatggcg gccgcgataa gtcagattaa ccaggctaac
      361 agccagatgc aggccctgaa cagcgctgcc gaagccgccg gcgccct
//
LOCUS       DAZGAP010000614          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZGAP010000614 DAZGAP010000000
VERSION     DAZGAP010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.620"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          114 a           71 c           96 g          123 t
ORIGIN      
        1 atgacgaatc ctccatttca ggatttttta tgtaacgtct actacatgcg atccgttggt
       61 gaaccgttag ttatatattg gttatatgtt agttatatct tagttatact tttgctgcgc
      121 gtcagttaca gaggcgtttt gagggtaaat tctggaggca cgtgaccgac tgtatacaaa
      181 atgctggcgc attttgatac gtcgacgtgc caaaggggac gcccctttga aaccccgtca
      241 ggggacggac gacctggcgg tggcattgtt aattatttac aggaggtatc ctgtggtcac
      301 taaaaaaaag gacaaaatta acagggatgt cgttatatcc ttaagattga cagagaaaga
      361 atttggcgat tttgttgatg atataaaaaa atctcaactc agtc
//
LOCUS       DAZGAP010000615          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZGAP010000615 DAZGAP010000000
VERSION     DAZGAP010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.621"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>404
                     /locus_tag="ACHLAM_005630"
     CDS             <1..>404
                     /locus_tag="ACHLAM_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7856957.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKV"
BASE COUNT           76 a          121 c          115 g           92 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggcggt
      301 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      361 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag taca
//
LOCUS       DAZGAP010000616          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZGAP010000616 DAZGAP010000000
VERSION     DAZGAP010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.622"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..>403
                     /locus_tag="ACHLAM_005631"
     CDS             51..>403
                     /locus_tag="ACHLAM_005631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HFO7856958.1"
                     /translation="MIFAEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVAS
                     PEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKDIYQLIKNDW
                     KKVGSLPEGLAYGVSL"
BASE COUNT          112 a           72 c          112 g          107 t
ORIGIN      
        1 agtggaagaa cctgggaacg actccatttc cgggaactgc tggttcatcg gtgatttttg
       61 cagaacagca aatttatata ctgggagggg agagaaaacc ggggcttcgt tctgttagat
      121 cctggacggg ggagctttcg catgatcgaa ttaaatggtc tgaacttcca ccagtagctt
      181 cacccgaggg ggtgtcagga gcatatgcct ctgttattga tggaaatatc tttctggccg
      241 gtggtgcata tttcccgggg gcggcagaaa agtatagcaa tggtgaatac tggtctcata
      301 aaggactgga taaagcctat tcaaaagata tatatcagct aataaaaaat gactggaaaa
      361 aagttggttc tttgccggaa ggacttgctt atggtgtaag cct
//
LOCUS       DAZGAP010000617          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZGAP010000617 DAZGAP010000000
VERSION     DAZGAP010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.623"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..>403
                     /locus_tag="ACHLAM_005632"
     CDS             93..>403
                     /locus_tag="ACHLAM_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006777722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4526/YPO1902 family pathogenicity island
                     replication protein"
                     /protein_id="HFO7856959.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FG"
BASE COUNT           90 a           94 c          133 g           86 t
ORIGIN      
        1 gggtatgagg ccgggaaaaa tgcctcactg catcatggtg tgggggagag tgcccgacag
       61 gtggtgccgg gtctgatgat acgggaggcg tcatgaataa cagcttgtct caggcaacca
      121 acggtctgct gatgcaactg gtgatggacc tcaagagcgg ctacctgcgt cgctgcgaat
      181 ctctggggct ggcccgggag gaaatgcaga tgctgcaggg actgaccatt gaggaaattc
      241 attacctgtc aaactctgaa gtgtcggtta tccggctgga cattaatcat gaaaacctgc
      301 tgcgtatgct gcagcaggcc cggacggagc agaaacgtct gcagcgcatt gaccgggcac
      361 tggcgctggg aggctccatt gaactgatgg cgttttactt tgg
//
LOCUS       DAZGAP010000618          400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZGAP010000618 DAZGAP010000000
VERSION     DAZGAP010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.624"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..>400
                     /locus_tag="ACHLAM_005633"
     CDS             71..>400
                     /locus_tag="ACHLAM_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856960.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDETDKGYQVYG
                     DNWKSIEIGTTKEVKKKNSTDLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKTG
                     ESIAIGAK"
BASE COUNT          153 a           65 c           85 g           97 t
ORIGIN      
        1 taatattcat gcatgatttt tatgctattc cgactgagtg gaagaaaata aaggagaaaa
       61 gaggatatat atgaacctca gaaaaaaaca tatctgtata gccatatcac ttgttcttaa
      121 tccgttccct gcatcactgg cagcacaaaa cactaatgta gttgaagttg atgaaacaga
      181 taaaggttac caagtttatg gtgacaactg gaaaagtatt gaaataggta caacgaagga
      241 agtaaagaaa aagaattcaa ccgaccttaa ttttgaccat aactccccgg tagcgaaagg
      301 gcataactcc attgcgattg gtaccagtgc agaagcagga ggtgaaatta aagacgaaaa
      361 aggttttaag acaggagaat ccatagctat tggtgccaag
//
LOCUS       DAZGAP010000619          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZGAP010000619 DAZGAP010000000
VERSION     DAZGAP010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.625"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>399
                     /locus_tag="ACHLAM_005634"
     CDS             <1..>399
                     /locus_tag="ACHLAM_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856961.1"
                     /translation="KGGWNFYQYPLNPITNIDPLGLETLKCIKPLHSMGGTGERSGPD
                     IWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVET
                     DNSCVENCLKGKFKEVRPRYSVLPDIFTPIN"
BASE COUNT          126 a           64 c          105 g          104 t
ORIGIN      
        1 aaggggggat ggaattttta tcagtatccg ttgaatccga tcactaatat agatccttta
       61 ggattagaaa cactaaaatg cattaagcca ctgcattcaa tgggcggaac tggtgaaaga
      121 agcggtccag atatatgggg gaatccgttc tatcatcaat atctttgtgt cccagatggt
      181 aaaggggact atacttgtgg tggccaagac caacggggag aatcaaaagg agatggtcta
      241 tgggggccag gtaaagcaag taatgataca aaagaagctg ctggccgttg tgacctcgtt
      301 gaaaccgata atagttgtgt ggagaactgt ttaaaaggga agtttaaaga ggtaaggccg
      361 cgttattctg tattgcctga tatattcaca cctataaat
//
LOCUS       DAZGAP010000620          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZGAP010000620 DAZGAP010000000
VERSION     DAZGAP010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.626"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..370)
                     /locus_tag="ACHLAM_005635"
     CDS             complement(<1..370)
                     /locus_tag="ACHLAM_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856962.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHD"
BASE COUNT           95 a          125 c           91 g           87 t
ORIGIN      
        1 tgtcgtgccg ggcaaaatat tcatccgctc cggccaccat ctttccggta ctgatactgt
       61 cagcccagat ttcatgatta atttcacgcc ccagagccat cagactgtcg agtgcctgac
      121 gggcctgaat tcccggtgcg gaaatctcct cctgctccag gtcatacaac agccagccca
      181 gcgcctcgcc ggtatcccac agatacacgt ataccagtga aggctccgtc tcatcatccg
      241 gctccggggt aatacccagc cgttgccagg cacgtaaccg catcagctcg ccttccatct
      301 gactgatatg agccagcaga gcagactgcg tctgaagcgc tgaataatga gcaattaaag
      361 gtgaatacat gatgacctcc gtctcttaaa ggaagtcc
//
LOCUS       DAZGAP010000621          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZGAP010000621 DAZGAP010000000
VERSION     DAZGAP010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.627"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..347)
                     /locus_tag="ACHLAM_005636"
     CDS             complement(<1..347)
                     /locus_tag="ACHLAM_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856963.1"
                     /translation="MFFKTSNPSALAAWQKYQQDYKKVKDEAKRLEAVLNVDCRSVFV
                     SGINGFYFKGLRFTDNKYPFHRDLWRKPTASNGWSCTPRTSCIPKALCAASDELNSLW
                     REYSPVTYARTDAL"
BASE COUNT          102 a          104 c           95 g           94 t
ORIGIN      
        1 agtgcatcgg ttctggcata cgtgacgggc gaatattcac gccacagact gttaagttcg
       61 tcagaggcag cgcacagggc tttgggaata cacgatgtgc gcggcgtgca gctccagcca
      121 ttcgacgcag tcggttttcg ccataagtcg cgatgaaaag gatatttgtt atccgtaaaa
      181 cgcagtcctt taaaataaaa accattgata ccggatacaa ataccgaccg acaatcaaca
      241 ttcagcacgg cctcaagacg ttttgcctca tccttaactt tcttgtagtc ctgctggtat
      301 ttttgccacg cagccagcgc ggaagggttt gatgttttaa agaacattac gccacctccg
      361 cagatatccc ggcaggcgca gccgtccggt caaca
//
LOCUS       DAZGAP010000622          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZGAP010000622 DAZGAP010000000
VERSION     DAZGAP010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.628"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>395)
                     /locus_tag="ACHLAM_005637"
     CDS             complement(<1..>395)
                     /locus_tag="ACHLAM_005637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856964.1"
                     /translation="GVIKVLPVFTLIQTTIFSIPGVALFPAVIGTAILSGIVGSTSGG
                     GGLVMVTFGQDLLELSQAQNISPGLMHRIIVFSASTLDTLPHSGFIITLLGVCGLSHK
                     QSYKELFIVTCVFPAFAVLSMLVYAFLFL"
BASE COUNT          152 a           80 c           69 g           94 t
ORIGIN      
        1 ttaaaaataa gaatgcatat accaacatgg agagaaccgc aaatgcggga aacacacaag
       61 taacaataaa aagctcttta taactttgct tatgagataa gccacaaact cctagcaatg
      121 tgataataaa gccagaatga ggaagcgtat ccaacgttga tgcactaaaa acaataatac
      181 gatgcattaa acctggcgaa atattctgag cttgagatag ttctaataag tcttgaccaa
      241 acgttaccat aactaatccc ccccctcctg atgttgagcc cactattccc gaaagaattg
      301 ctgtacctat aactgcagga aatagtgcta ctccaggaat agaaaaaatt gttgtttgaa
      361 taagggtaaa cacaggaagg actttaatta cccca
//
LOCUS       DAZGAP010000623          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZGAP010000623 DAZGAP010000000
VERSION     DAZGAP010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.629"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..306)
                     /locus_tag="ACHLAM_005638"
     CDS             complement(<1..306)
                     /locus_tag="ACHLAM_005638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856965.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVSDIIGLSHDEFNQQYKGNLTRQQHLRDI
                     "
BASE COUNT          114 a           69 c           77 g          129 t
ORIGIN      
        1 aatatctctc aggtgttgtt gtctggttaa attaccttta tattgttgat tgaactcatc
       61 atgagataaa ccgatgatgt cagaaacaac atcgggagat aaatgattga gaagagattt
      121 tgctattgca tgtttatatc gttccctgat aatcactgaa aaattggaca tgtctgcacc
      181 ggatattatc tcagtagtga tagtggatac acttttcgtc ataagtaatt gccatgccac
      241 ttcaagagaa gcatcatgca taccatcaaa acgggctctt tccttcagtt cagaacatgt
      301 tgtcattgaa ctcacctgcg aattttgtga tgtgtgtata attataaagt gggggtgaaa
      361 ccacctccac tttgttatca tgcatgtgt
//
LOCUS       DAZGAP010000624          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZGAP010000624 DAZGAP010000000
VERSION     DAZGAP010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.631"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..>383
                     /locus_tag="ACHLAM_005639"
     CDS             117..>383
                     /locus_tag="ACHLAM_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HFO7856966.1"
                     /translation="MIIKPVIKGVVSKSAHPEGCRHAIMEQIKYCQSHLQQINNGPKR
                     VLILGASSGFGLAARISLTFGGAKCDSIGVSFEKGPSEKSIGTAG"
BASE COUNT          119 a           70 c           81 g          113 t
ORIGIN      
        1 aaatattgta aagctttcaa caatcacaac aaatcaaaat gatgaggttg taatcgaagg
       61 cattgcagta gtcatggctc caaaatctta atttatttaa ctatcaagga ccttatatga
      121 ttataaaacc tgtaattaaa ggtgttgtct cgaaaagtgc tcatccagag ggatgtcgtc
      181 atgcgataat ggaacagatt aagtattgcc agtcccatct tcagcaaatt aataatggcc
      241 cgaaacgagt tctgattctc ggggcatctt caggatttgg tcttgctgca aggatttctc
      301 tcacttttgg cggcgcaaag tgcgattcta tcggagtttc gtttgaaaaa ggtccctctg
      361 agaaatctat aggaactgca gga
//
LOCUS       DAZGAP010000625          379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZGAP010000625 DAZGAP010000000
VERSION     DAZGAP010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.632"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>379)
                     /gene="cbi"
                     /locus_tag="ACHLAM_005640"
     CDS             complement(<1..>379)
                     /gene="cbi"
                     /locus_tag="ACHLAM_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin B immunity protein"
                     /protein_id="HFO7856967.1"
                     /translation="ANEILYAFSIIGIIPLMAILILRINNPYSQVLYYLYNKVAFLPS
                     ITSLYDPVMTTLMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQSCFWGPVFYA
                     ILIYITLFYNLELTTAGGFFKLLS"
BASE COUNT          131 a           55 c           72 g          121 t
ORIGIN      
        1 gataataatt taaaaaaacc acctgctgtt gttagttcca gattatagaa cagtgtgata
       61 taaatcagaa tggcataaaa aacgggcccc cagaaacagg attgcacaac aagttttctt
      121 gttactggct ttatgatttc tcttgtctta tatgtgcaaa gaaaaacgag aatacccata
      181 actggtgctg tcttgttgta gtttgacata agtgttgtca tgacgggatc atacaatgat
      241 gtaatagaag ggagaaatgc caccttatta tataagtagt acagcacttg agaatatgga
      301 ttatttattc gaagtattaa tatagccatt aatggaataa tcccgattat ggaaaatgca
      361 tataatattt cgtttgctt
//
LOCUS       DAZGAP010000626          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZGAP010000626 DAZGAP010000000
VERSION     DAZGAP010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.633"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>378
                     /locus_tag="ACHLAM_005641"
     CDS             <1..>378
                     /locus_tag="ACHLAM_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7856968.1"
                     /translation="GWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEGGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAGR"
BASE COUNT           93 a          103 c          131 g           51 t
ORIGIN      
        1 ggctggacgc cgcgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
       61 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      121 ccggaaatcc gttaccgtta cgacagtgac gggcgggtga cagaacagct aaacccggca
      181 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
      241 cgccgtgaag tgctgcacac gcagggcgaa ggcgggctga agcgggtggt gaaaaaggaa
      301 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct taaggcacag
      361 acggatgccg caggccgg
//
LOCUS       DAZGAP010000627          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZGAP010000627 DAZGAP010000000
VERSION     DAZGAP010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.634"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>376
                     /locus_tag="ACHLAM_005642"
     CDS             <1..>376
                     /locus_tag="ACHLAM_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7856969.1"
                     /translation="PLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDG
                     WVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRH
                     VLGVIADWPVNRVSELLPWRIAL"
BASE COUNT           86 a           97 c          115 g           78 t
ORIGIN      
        1 accgctgttg aaatccctgg aaagctggtt gcgtgaaaag atgaagaccc tgtcgcgaca
       61 ctcagagttg gcgaaggcgt tcgcgtacgc acttaaccag tggccggcac tgacgtacta
      121 tgcgaacgat ggctgggtgg aaatcgacaa caacatcgct gaaaatgccc tgcgggcggt
      181 cagtctgggt cgtaaaaact tcctgttctt cggctctgac catggtggtg agcggggagc
      241 gctactgtac agcctgatcg ggacgtgcaa actgaatgac gtggatccag aaagctacct
      301 tcgccatgtg cttggcgtca tagcagactg gccggtcaac cgggtcagcg aactgcttcc
      361 gtggcgcata gcactg
//
LOCUS       DAZGAP010000628          373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZGAP010000628 DAZGAP010000000
VERSION     DAZGAP010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.635"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          105 a           77 c           83 g          108 t
ORIGIN      
        1 aatggatgtg ttgtttgtgt gatactgtaa agttggtaga ttgtgactgg cttaaaaaat
       61 cattaattaa taataggtta tgtttagaga gttccccgcg ccagcgggga taaaccgttg
      121 atcgccgtgg cggtgagatt agaaacgcgg agttccccgc gccagcgggg ataaaccgga
      181 agtggcaaag ctgccgtttc ttttctctat gagttccccg cgcctgcggg gataaaccac
      241 catataaccc gttatctctt tctcaagttt ttatattagc tgtacttgta ataagcaaca
      301 tatccacgta acacctcatg ttcaaaatag ttctccatgc cagagaagtt cacaattatc
      361 gatacaaaaa att
//
LOCUS       DAZGAP010000629          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZGAP010000629 DAZGAP010000000
VERSION     DAZGAP010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.636"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..303)
                     /locus_tag="ACHLAM_005643"
     CDS             complement(<1..303)
                     /locus_tag="ACHLAM_005643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856970.1"
                     /translation="MSTGPREPEGLPLLESGGGNGGVFYDHEPLLLIASRRLRASLET
                     ALIVLQKWTVCVGCGYSGVRDGACKNAPVWVKVNPESGLGLCSGCDAFLLQERQRRQ"
BASE COUNT           94 a          122 c           83 g           73 t
ORIGIN      
        1 ctgacgacgc tggcgttcct gcaaaagaaa ggcgtcacaa ccggaacaca gccccagacc
       61 actctcagga ttcaccttca cccagaccgg agcattctta caggcaccat cccgtacccc
      121 ggaataacca cagcccacac acaccgtcca cttctgcagt acgatgagtg cagtttcgag
      181 tgatgcgcgg agacgccggg aggcaataag gagcaacggc tcatggtcat aaaaaacccc
      241 gccgttcccc cctccggatt caagcagcgg gagtccttca ggctcccggg gacctgttga
      301 catttcccgt aattcgtttt cattccacat attatggaaa gttatccggt tcactgaaca
      361 acgccattcc tc
//
LOCUS       DAZGAP010000630          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZGAP010000630 DAZGAP010000000
VERSION     DAZGAP010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.637"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..303)
                     /locus_tag="ACHLAM_005644"
     CDS             complement(<1..303)
                     /locus_tag="ACHLAM_005644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856971.1"
                     /translation="MSTGPREPAGLPLLESGGGNGGVFYDHEPLLLIASRRLRASLET
                     ALIVLQKWTVCVGCGYSGVRDGAGKNAPVWVKVNPESGLGLCSGCDAFLLQERQRRQ"
BASE COUNT           92 a          122 c           86 g           72 t
ORIGIN      
        1 ctgacgacgc tggcgttcct gcaaaagaaa ggcgtcacaa ccggaacaca gccccagacc
       61 actctcagga ttcaccttca cccagaccgg agcattctta ccggcaccat cccgtacccc
      121 ggaataacca cagcccacgc acaccgtcca cttctgcagt acgatgagtg cagtttcgag
      181 tgatgcgcgg agacgccggg aggcaataag gagcaacggc tcatggtcat aaaaaacccc
      241 gccgttaccc cctccggatt caagcagcgg gagtcctgca ggctcccggg gacctgttga
      301 catttcccgt aattcgtttt cattccacat attgtggaaa gttatccggt tcactgaaca
      361 acgccattcc tc
//
LOCUS       DAZGAP010000631          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZGAP010000631 DAZGAP010000000
VERSION     DAZGAP010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.638"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>371
                     /locus_tag="ACHLAM_005645"
     CDS             <1..>371
                     /locus_tag="ACHLAM_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO7856972.1"
                     /translation="IDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVV
                     CKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWH
                     EANLTPYLLTLLRGETKYGSL"
BASE COUNT          108 a           74 c           75 g          114 t
ORIGIN      
        1 aattgacccc aaatactttg ctgatctgcc gacgtcacag ttttggtcgt acgcaacata
       61 cttcagggta ttgtcttttg aatatctgag tgaaagtatt tctacactgc tgtatctgga
      121 tgccgatgtt gtttgtaaag gaagcctgaa acctctcaca gaaattatat ttaaagatga
      181 gtttgctgcg gtcattcctg acaatgatag tactcaggcg gcatgtgcaa aacgcctcaa
      241 cattcccgaa atgaatggac gttatttcaa tgcaggcgtt atctatgtca atcttaaaaa
      301 atggcatgaa gcaaatttga caccgtattt actcacgctt ttacgagggg aaactaaata
      361 tggctctctt a
//
LOCUS       DAZGAP010000632          354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZGAP010000632 DAZGAP010000000
VERSION     DAZGAP010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.639"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..192
                     /locus_tag="ACHLAM_005646"
     CDS             <1..192
                     /locus_tag="ACHLAM_005646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856973.1"
                     /translation="QKDTMYPGSSTPFSYFMRMASGGGDVRIKFRIEVVYYGTAPGFM
                     TTGIQITTMKATASGFVES"
BASE COUNT           95 a           83 c          105 g           71 t
ORIGIN      
        1 cagaaagaca ccatgtatcc cgggtcatcc actccgttca gctatttcat gaggatggcg
       61 tcagggggag gagatgtcag gatcaagttc cggattgagg tcgtctatta tggaactgca
      121 ccgggtttta tgaccacagg gatacagatc acaacgatga aggcgacagc ctccggattt
      181 gtggaaagtt aatccgggac gcccgggatt gccggaagga gacaaaaacc gtacagtatg
      241 cgcgggtgcc tttggctgat ggccggaggg aacacctgaa ggccggatgt gaaaaggccc
      301 cggacaaaac attcatgttt aacccgaggc ctgaccattc atcctaagca agtg
//
LOCUS       DAZGAP010000633          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZGAP010000633 DAZGAP010000000
VERSION     DAZGAP010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.640"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..192
                     /locus_tag="ACHLAM_005647"
     CDS             <1..192
                     /locus_tag="ACHLAM_005647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856974.1"
                     /translation="QKDTMYPGSSTPFSYFMRMASGGGDVRIKFRIEVIYYGTAPGFM
                     TTGIQITTMKATASGFMES"
BASE COUNT           98 a           81 c          100 g           74 t
ORIGIN      
        1 cagaaagaca ccatgtatcc cgggtcatcc actccgttca gctatttcat gaggatggcg
       61 tcagggggag gagatgtcag gatcaagttc cggattgagg tcatctatta tggaactgca
      121 ccgggtttta tgaccacagg gatacagatc acaacgatga aggcgacagc ctcaggattt
      181 atggaaagtt aatcccggac gatcgtgatt gccggaagga gacaaaaacc gtacagtatg
      241 cgcgggtgcc tttggctgat ggccggaggg aacacctgaa ggccggaaag acgaaggccc
      301 cggacaaaca ttcatgttta acccgaggcc tgactttcga tcttcagcaa att
//
LOCUS       DAZGAP010000634          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZGAP010000634 DAZGAP010000000
VERSION     DAZGAP010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.641"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..214
                     /locus_tag="ACHLAM_005648"
     tRNA            120..214
                     /locus_tag="ACHLAM_005648"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:154..156,aa:Sec,seq:tca)
BASE COUNT           78 a           90 c           87 g           94 t
ORIGIN      
        1 ctataatgtt ttcgtgataa tacgctgcgt gtattaggcg gaaaaaactg atctggggga
       61 tgtagaaact caaggaagta gctataatgc gccccgcctc catgtagcaa tcgaggcgcg
      121 gaagatcgtc gtctccggtg aggcggctgg acttcaaatc cagttggggc cgccagcggt
      181 cccgggcagg ttcgactcct gtgatcttcc gccaattaac atcttctgaa ctctcccgat
      241 gtcaatcccc cccttctaat cccttgtata tcaggattcc tttgtatcca gaagtcaacc
      301 gacttcaccc cgtatcaagt tgcttttggg ggtactttag ggggtatta
//
LOCUS       DAZGAP010000635          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZGAP010000635 DAZGAP010000000
VERSION     DAZGAP010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.642"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>348
                     /locus_tag="ACHLAM_005649"
     CDS             <1..>348
                     /locus_tag="ACHLAM_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFO7856975.1"
                     /translation="TKCFDIIFLGSDQIWNANYTNGVDPNYYGQGPYCKAHKLVSYAA
                     SMGKLCLGKYEEQAFLSLINNIQQIGVRENYLKTYIEEKTDLKCDVNLDPTLLLTKSD
                     WDKLAAPNDTQER"
BASE COUNT          124 a           51 c           69 g          104 t
ORIGIN      
        1 tactaaatgt ttcgatatta tctttcttgg tagtgatcag atatggaatg caaattatac
       61 taacggagta gaccctaact attatggaca aggaccttat tgtaaagcgc acaaattagt
      121 ttcttatgcg gcaagtatgg gtaagctgtg tttgggaaaa tatgaggaac aagcattctt
      181 atctttgata aataatatac agcagattgg tgttagagaa aattatttaa agacgtacat
      241 agaagaaaaa accgatttaa aatgtgatgt taatcttgat ccgactcttc tgcttactaa
      301 aagcgattgg gataaactgg cagcacctaa cgatacacaa gaacgcta
//
LOCUS       DAZGAP010000636          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZGAP010000636 DAZGAP010000000
VERSION     DAZGAP010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.643"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>338
                     /locus_tag="ACHLAM_005650"
     CDS             <1..>338
                     /locus_tag="ACHLAM_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFO7856976.1"
                     /translation="RSIKYQTRVFNEHINELVITRRNGKVITVNDGREMIEFVSCSYL
                     GLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEKRLNKIMNGAHTLTFNAVT
                     PCHLAVLPLL"
BASE COUNT          122 a           57 c           68 g           91 t
ORIGIN      
        1 aacgttcaat caaataccaa acaagagtat ttaacgaaca tataaatgaa ctagtgatca
       61 ccagaagaaa tggtaaggta atcactgtta atgatgggag ggagatgatt gagtttgtgt
      121 catgctctta tcttggactg gaaacacatg aaaatctaaa acatgcaatc gctaatgcaa
      181 ttgaaagatt tggagtccaa ctttcggtcg ccagaacgcg cgtaaaagta gatttattcg
      241 agcaattgga aaagagatta aataaaataa tgaatggggc gcacacatta acatttaatg
      301 cggttactcc atgccattta gctgtactcc cacttctt
//
LOCUS       DAZGAP010000637          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZGAP010000637 DAZGAP010000000
VERSION     DAZGAP010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.644"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>336
                     /locus_tag="ACHLAM_005651"
     CDS             <1..>336
                     /locus_tag="ACHLAM_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856977.1"
                     /translation="KQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGY
                     CSAQGEQWEYRYDASGRRTEKRCDRKKIRFTYLWDGDNIAEIREYRDDKLYSVRHLVF
                     NGFELISQQ"
BASE COUNT           88 a           89 c          109 g           50 t
ORIGIN      
        1 gaaacaggcc ggaaacacgc agtatgacta tgacgccgca ggccggatgg tcagccgcac
       61 cagacaccgt gacggctacc gcccggaaac agagcggttc cggtgggaca gccgtgacca
      121 gctgaccggg tattgcagcg cacagggtga gcagtgggaa taccgctacg acgccagcgg
      181 caggcggacg gaaaaacgct gcgaccggaa gaaaatccgt tttacgtacc tgtgggacgg
      241 cgacaatatt gcggaaatcc gggaataccg cgatgataaa ctgtacagcg tacggcacct
      301 ggtgtttaac ggctttgagc tgataagcca gcagtg
//
LOCUS       DAZGAP010000638          332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZGAP010000638 DAZGAP010000000
VERSION     DAZGAP010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.645"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>332
                     /locus_tag="ACHLAM_005652"
     CDS             <1..>332
                     /locus_tag="ACHLAM_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7856978.1"
                     /translation="RQQCKQIKSLTGFTLPVVLSAEFSGPETPWIIVRGDKPVVCPVN
                     HAPQAFTDWLQVEDNILALPVISEAFSFIRNTLADELEKADRLMPPVRAFSVVMRLGA
                     VLSGTPSV"
BASE COUNT           68 a           83 c           95 g           86 t
ORIGIN      
        1 cgccagcagt gtaagcagat aaaatccctg acgggtttca cactgccggt ggtgttgagt
       61 gccgaatttt ccgggcctga aacaccgtgg ataattgtcc ggggggataa gccagttgta
      121 tgtccggtta atcatgcacc tcaggcattc actgactggc tgcaggtgga agacaatatt
      181 ctggcactgc cggtcatcag cgaggcattt tcctttatcc gtaatacgct ggctgatgag
      241 ctggaaaaag ctgaccggct gatgccacct gtacgggcat tttccgttgt aatgcgcctt
      301 ggtgccgtcc tttcggggac tccgtcagtc tg
//
LOCUS       DAZGAP010000639          332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZGAP010000639 DAZGAP010000000
VERSION     DAZGAP010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.646"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..193
                     /locus_tag="ACHLAM_005653"
     CDS             <1..193
                     /locus_tag="ACHLAM_005653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856979.1"
                     /translation="DVLTRLPEWPEERLHDLLPFSAYRFAELTDVTVIPPNAGKRVME
                     VVSSTPMNDTASEVKIPTQ"
BASE COUNT          103 a           87 c           77 g           65 t
ORIGIN      
        1 ggatgtcctg acacgtctgc ctgaatggcc ggaagagcgc ttgcacgact tactaccgtt
       61 ctcagcctac cgattcgcag aattaactga cgttacggtc ataccaccta atgcagggaa
      121 acgagtcatg gaggtcgtca gctctactcc catgaatgac acagcctctg aagtaaaaat
      181 accgacgcag tgaaaccagc cttaagcttt ccaaaacaca attacaacgt aagcgcacag
      241 cctgaaccgt ctggtcagaa tctgacgaat tagacaaagt ggtgtccacc aaataagtag
      301 tgggaaccaa aggtaagcgc accgtgaagg ac
//
LOCUS       DAZGAP010000640          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZGAP010000640 DAZGAP010000000
VERSION     DAZGAP010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.647"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>317
                     /locus_tag="ACHLAM_005654"
     CDS             <1..>317
                     /locus_tag="ACHLAM_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7856980.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPL"
BASE COUNT           62 a           88 c           94 g           73 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgct
//
LOCUS       DAZGAP010000641          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZGAP010000641 DAZGAP010000000
VERSION     DAZGAP010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.648"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>317
                     /locus_tag="ACHLAM_005655"
     CDS             <1..>317
                     /locus_tag="ACHLAM_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO7856981.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVY"
BASE COUNT           80 a           71 c           90 g           76 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacat
//
LOCUS       DAZGAP010000642          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZGAP010000642 DAZGAP010000000
VERSION     DAZGAP010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.650"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(166..240)
                     /gene="tnaC"
                     /locus_tag="ACHLAM_005656"
     CDS             complement(166..240)
                     /gene="tnaC"
                     /locus_tag="ACHLAM_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO7856982.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
BASE COUNT          117 a           52 c           54 g           88 t
ORIGIN      
        1 aatatccatt aattcgccag atatatatgc aagcaccatc atttactaaa acaagtaaat
       61 aaacgaaaga tggcctttgt aaaaggcaaa attaagatat agcaaacaaa tagatcacat
      121 tgaatctaat cggtttggct ctggtgatgg ctacagaagg gcaaatcaag ggcggtgatc
      181 gacaatcttg ttgtcaatat taaaccattt tgaggtcaca catatatgta agatattcat
      241 aatgcactta tcctcgaaag acacgaggct acagtataat actcaagaaa tttgtctatt
      301 ctgaaattgt t
//
LOCUS       DAZGAP010000643          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZGAP010000643 DAZGAP010000000
VERSION     DAZGAP010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.651"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..230
                     /locus_tag="ACHLAM_005657"
     CDS             <1..230
                     /locus_tag="ACHLAM_005657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7856983.1"
                     /translation="PFIIRNIAVSNGQYVNSGDDIVDIELLDKFFIDVKIDPVSEQGN
                     IKEKKIRYHSLVNGLSGLASVVRITRTFENG"
BASE COUNT          100 a           49 c           65 g           97 t
ORIGIN      
        1 cgccttttat cataaggaat attgctgtaa gcaatgggca atatgtcaat tcaggagacg
       61 atatcgtaga tattgaactt ctggataagt ttttcattga tgttaaaatt gatcctgtgt
      121 ctgaacaagg aaatatcaaa gaaaaaaaga taagatatca ctctttggtc aatggattat
      181 ccggtttagc atctgtagtc cgcatcaccc gtaccttcga aaatggctag gatgataaaa
      241 gttctggttt gagggttgta acattggcca ttgatggtga tcataaaaaa ctcgctgaat
      301 tactcgatac t
//
LOCUS       DAZGAP010000644          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZGAP010000644 DAZGAP010000000
VERSION     DAZGAP010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.652"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            28..>311
                     /locus_tag="ACHLAM_005658"
     CDS             28..>311
                     /locus_tag="ACHLAM_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7856984.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLT"
BASE COUNT           89 a           61 c           89 g           72 t
ORIGIN      
        1 gggatgcagg tttcacagga gaacgtcatg agcagttcgt ttcagaatga gatcccgaag
       61 gcaagggtga atctgaaatt agacctacat acaggtggtg ggcagaagaa agtggagctt
      121 ccgctcaaac tgctggttgc cggtgatttc agtaacggac aggaagagcg cccgctgtca
      181 gagcgcataa aaatggatgt taataaaaat aactttaatg ctgttctggc tgatatctcg
      241 ccgtcagtac gcctgaatgt ggagaacaca cttgccggtg atggtactga agagagcatc
      301 agcctgactt t
//
LOCUS       DAZGAP010000645          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZGAP010000645 DAZGAP010000000
VERSION     DAZGAP010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.653"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>306
                     /locus_tag="ACHLAM_005659"
     CDS             <1..>306
                     /locus_tag="ACHLAM_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856985.1"
                     /translation="TAKQNADTLKGEADSAVKALPDTAAEKGGLQGRVDALDGIQVPE
                     VNDQDGNGKADAEEAAAAVAAATEKVEAAEAADKAAKDKLAELNADKLITPEEKAQL"
BASE COUNT           84 a           67 c          122 g           33 t
ORIGIN      
        1 aaacggcgaa gcagaatgct gacaccctga agggtgaggc agacagtgcc gtgaaggcgt
       61 tgccggatac agccgcggag aaaggtggcc tgcagggtcg tgtggatgca ctggatggta
      121 tccaggtacc ggaagtgaac gatcaggacg gcaacggcaa ggcggatgca gaggaagcgg
      181 cagcagcggt ggcggcggcc acggagaaag tggaagcggc agaagcggcg gacaaggctg
      241 cgaaggacaa gcttgcagag ctgaatgcag acaagctgat cacgccggaa gagaaggcgc
      301 agctgg
//
LOCUS       DAZGAP010000646          305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZGAP010000646 DAZGAP010000000
VERSION     DAZGAP010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.654"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>305)
                     /locus_tag="ACHLAM_005660"
     CDS             complement(<1..>305)
                     /locus_tag="ACHLAM_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856986.1"
                     /translation="KTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTF
                     KIAATHLRDLALFDYPSSATLYMTNEQYIMAGIRYNRGVERDLGFFIYLINNLPARD"
BASE COUNT          105 a           59 c           53 g           88 t
ORIGIN      
        1 atcacgcgca ggcaaattat taattaaata aataaaaaaa cccaaatctc gttcaacacc
       61 acgattatat ctaataccag ccataatata ctgctcattc gtcatataca gtgttgccga
      121 agagggataa tcaaaaaggg caagatctct taaatgagta gcagcaattt taaaggtaaa
      181 atcatcttcc atcaggcatg tcgctaactc taactgattt ctggtagtta attctgaagg
      241 gtcaaggcct agagtaatcg ctgcggttcg tatttgtata cccattgagc caaatgatgt
      301 tttat
//
LOCUS       DAZGAP010000647          304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZGAP010000647 DAZGAP010000000
VERSION     DAZGAP010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.655"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..304)
                     /locus_tag="ACHLAM_005661"
                     /pseudo
     CDS             complement(1..304)
                     /locus_tag="ACHLAM_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           72 a           79 c           78 g           75 t
ORIGIN      
        1 agaatgccgg gagcctgcag gggcgcttcg tgaatttcac ggatacattc tgcgtcatga
       61 aaattttctc tgtaccgggt tcattccggc agtatgtggt gcatatggag cattctgttc
      121 cgtgcggcgc tgagctcatc aatatagcgc ccccggaaaa cgggaccgtc atgcatatag
      181 cacagaggga acacttccac attgtcacaa tattcccgcc cccactcttt tgtgacggag
      241 gcgtcatcgg tgtaaaacca tgtgtcgggc atgccgttag ccagaaagca aatgttccat
      301 acat
//
LOCUS       DAZGAP010000648          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZGAP010000648 DAZGAP010000000
VERSION     DAZGAP010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.656"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>301)
                     /locus_tag="ACHLAM_005662"
     CDS             complement(<1..>301)
                     /locus_tag="ACHLAM_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856987.1"
                     /translation="RFFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQRAARLAAAVK
                     RYKTSEMLRFIFATTAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQH"
BASE COUNT           74 a           85 c           71 g           71 t
ORIGIN      
        1 ggtgctgacg ccccttctcc ccaaaaactt caacaaccag ccactggctt cctgttctgc
       61 cgaacaatgc cttgcacaaa cgtctcacag tcagcggggt taaatctgga tcgggatcgt
      121 aggcagtggt agcaaaaata aagcgcagca tttctgatgt cttgtaacgc tttacggcag
      181 ctgccagacg tgcggcgcgt tggggtgctt cccagcggtc gtcactgtaa ttagcaactt
      241 cagccagata atccttttca aggttttcag ccgtgtaaca cctgccgcct aaaaaaaacc
      301 g
//
LOCUS       DAZGAP010000649          296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZGAP010000649 DAZGAP010000000
VERSION     DAZGAP010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.657"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..>296
                     /locus_tag="ACHLAM_005663"
     CDS             4..>296
                     /locus_tag="ACHLAM_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HFO7856988.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDE"
BASE COUNT           62 a           76 c           94 g           64 t
ORIGIN      
        1 ctgatgaaac gacatctgaa tacctgctac aggctggtat ggaatcacat tacgggcgct
       61 ttcgtggttg cctccgaact ggcccgcgca cggggtaaac gtggcggtgt ggcggttgca
      121 ctgtctcttg ccgcggtcac gtcactcccg gtgctggctg ctgacatcgt tgtgcacccg
      181 ggtgaaacag tgaatggcgg aacactggta aaccatgaca accagtttgt atccggaaca
      241 gctgatggcg tgactgtcag taccgggctt gagctggggc cggacagtga cgaaaa
//
LOCUS       DAZGAP010000650          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZGAP010000650 DAZGAP010000000
VERSION     DAZGAP010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.658"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..>295
                     /locus_tag="ACHLAM_005664"
     CDS             67..>295
                     /locus_tag="ACHLAM_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO7856989.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKS"
BASE COUNT           78 a           52 c           75 g           90 t
ORIGIN      
        1 agtcaattcg ttgtgtattt atttatacaa tggtatcggt gtcctggaat ttaattagag
       61 gaatctatgc gagggaaaaa acgcattggg ttattgtttt tgctgatagc ggttgtggtt
      121 ggtggcggcg ggttattact ggcgcaaaaa gccttacata aaacgtcgga taccgcattt
      181 tgcctttcct gccactcgat gagtaaacct tttgaggaat atcagggaac tgtccacttt
      241 tcaaaccaga aagggatacg tgcggaatgt gccgattgcc atattccaaa gtcag
//
LOCUS       DAZGAP010000651          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZGAP010000651 DAZGAP010000000
VERSION     DAZGAP010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.659"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..295
                     /locus_tag="ACHLAM_005665"
                     /pseudo
     CDS             1..295
                     /locus_tag="ACHLAM_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          115 a           51 c           57 g           72 t
ORIGIN      
        1 gaaataaatt taaatcaatc atactttcga tataaagagc ctcgtaggcc gggatgtaag
       61 attcatgatc ctaagagtaa aataagtata actcaaagaa taataaaaag cataattcac
      121 caccggcact ggctatatat cttctgaaac ttgatgtacc tgaaggaaat tacgactctt
      181 ctggcagaag aattaataaa caggcgcaag atgatgaaaa agaattgggg ggcaaagtac
      241 accctgtgtc atcttcaagc atcaaaccgg tagtagacta ctacattaat ggaga
//
LOCUS       DAZGAP010000652          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZGAP010000652 DAZGAP010000000
VERSION     DAZGAP010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.660"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>292)
                     /locus_tag="ACHLAM_005666"
     CDS             complement(<1..>292)
                     /locus_tag="ACHLAM_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO7856990.1"
                     /translation="ESGLGRPQVTALLALRQDAERVWGQFAVAADTDAEFDRVFGESC
                     RKFNSPELWSLEMFRDELIGDLLQALPHPSLDYDRWLLELDPKERNRRHHFGE"
BASE COUNT           69 a           93 c           71 g           59 t
ORIGIN      
        1 gctcaccaaa atgatgacga cggtttcgct ctttcggatc cagttccagc aaccaccggt
       61 catagtccag ggagggatgt ggcagggcct gcaacaaatc tccgataagc tcatcgcgga
      121 acatctccag tgaccacagt tccggcgaat taaacttccg gcagctttcc ccgaacacgc
      181 ggtcaaattc cgcatccgta tcggctgcaa cagcaaactg cccccataca cgttctgcgt
      241 cctgacgaag tgcgaggagt gctgtaacct gtggacgccc cagtcctgac tc
//
LOCUS       DAZGAP010000653          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZGAP010000653 DAZGAP010000000
VERSION     DAZGAP010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.661"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>290
                     /locus_tag="ACHLAM_005667"
     CDS             <1..>290
                     /locus_tag="ACHLAM_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7856991.1"
                     /translation="ASAFTTGADGTYSESAGASENSLRWGVGKPLYQDLVSRTKAALA
                     KNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRTELAGQASQSTGG"
BASE COUNT           67 a           66 c          104 g           53 t
ORIGIN      
        1 gtgcttcggc attcacaacg ggtgcagacg gcacatacag cgaatcagcc ggtgcatcgg
       61 aaaattcact gcgctggggt gtgggtaagc cgctgtatca ggatttggtg agccggacta
      121 aagccgcact ggcgaagaac ccgaaaaacc gtctgcttgc ggtggtgtgg atgcagggag
      181 aaggtgatgc ggcagtgggg acgcatgcgc agcatccggg gctgtttagt gcgatggtga
      241 atcagttcag aacggagctt gccggtcagg catcacagag cacaggaggc
//
LOCUS       DAZGAP010000654          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZGAP010000654 DAZGAP010000000
VERSION     DAZGAP010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.662"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..>287
                     /locus_tag="ACHLAM_005668"
     CDS             11..>287
                     /locus_tag="ACHLAM_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO7856992.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYY"
BASE COUNT           66 a           65 c           94 g           62 t
ORIGIN      
        1 gatgagaatg atgagtgata tgtcgcatca ggaacgtctg atcaggcggg tcaggaaact
       61 gctggagctg agccgtaata acagcaatgc ccatgaggcc gggctggcgc ttgcaagggc
      121 gcaacagctg atggcgaaac acggtatcac ggagcaggat gccggacttt cttcggtgca
      181 gacctcttca tcacaggggg caccttctga tgctgaaaaa gttccggcgt ggatgtttcg
      241 tctggtcatg gtggtgacac agacatttgg ctgccgggcg tattacg
//
LOCUS       DAZGAP010000655          286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZGAP010000655 DAZGAP010000000
VERSION     DAZGAP010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.663"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>286
                     /locus_tag="ACHLAM_005669"
     CDS             <1..>286
                     /locus_tag="ACHLAM_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856993.1"
                     /translation="SQYLTSVILNNLPERPFNIRMVRETADSTTDQLQNRTLWSSYTE
                     IIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRT"
BASE COUNT           71 a           80 c           81 g           54 t
ORIGIN      
        1 cctcgcagta cctgacgtcg gtgattctga ataatctccc tgagcgcccc tttaacatcc
       61 ggatggtcag ggagacggcg gacagcacca cggaccagct gcagaacaga acgctgtggt
      121 cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcc attgtggggc
      181 tgcaggtgga tgcggagcag tttggtggcc agcagctgac ggtgaactac catatccgtg
      241 gtcgcatcat tcaggtgccg tcaaactatg acccggaaaa acgcac
//
LOCUS       DAZGAP010000656          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZGAP010000656 DAZGAP010000000
VERSION     DAZGAP010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.664"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..>285
                     /locus_tag="ACHLAM_005670"
     CDS             75..>285
                     /locus_tag="ACHLAM_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO7856994.1"
                     /translation="MSATPSPLRYPGGKAAIQDMISAIICSNNLTGGHYAEPYAGGGG
                     LALSLLFKQYIHEIHLNDLDRSVWSF"
BASE COUNT           89 a           59 c           51 g           86 t
ORIGIN      
        1 acagtagctc tgtccataag taatcaactg ttctaaaata aatcggtatg aatctatttt
       61 cacagggaaa gactatgtca gcaactccat caccattacg atatccggga gggaaagccg
      121 caattcaaga tatgatttcg gctataattt gctccaacaa ccttactggc ggacactatg
      181 cagaacccta cgctggaggt ggtggtcttg ctttatcatt attattcaaa caatatatac
      241 atgaaataca tttaaatgat cttgatcgct cagtttggtc attct
//
LOCUS       DAZGAP010000657          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZGAP010000657 DAZGAP010000000
VERSION     DAZGAP010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.665"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..>285
                     /locus_tag="ACHLAM_005671"
     CDS             53..>285
                     /locus_tag="ACHLAM_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFO7856995.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSG"
BASE COUNT           89 a           43 c           67 g           86 t
ORIGIN      
        1 tatgtaacaa atgcagattc ctgtcagaaa catgttataa ggaaatagta atatggccaa
       61 aatgattccg gcatttggac ccattgataa taatagttat ggggaaaaaa cagtctatac
      121 actgttaaaa gaaggtctgt ccgatgattt cacagtgatt catagtctcc cgtggttaag
      181 tgcggcgaca cgaaatttag ggatgaaatt acctccgagt ggagaaattg attttcttgt
      241 tctccatcct gtatatggtg ttttggcgtt agaggtaaag agtgg
//
LOCUS       DAZGAP010000658          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZGAP010000658 DAZGAP010000000
VERSION     DAZGAP010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.666"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>281
                     /locus_tag="ACHLAM_005672"
     CDS             <1..>281
                     /locus_tag="ACHLAM_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7856996.1"
                     /translation="GFGIKMKLLPQTENPLVEASVASTEKKKKTLSDNQMLRVYLVVQ
                     RFAEAEEQNMVNSRRRNAIYPAWYWLTVLDVLRYTGMRFNQLKHIRVRDV"
BASE COUNT           82 a           53 c           80 g           66 t
ORIGIN      
        1 ggattcggga tcaagatgaa actgctgcct cagacagaaa acccgttggt ggaagcatca
       61 gttgcgagta ctgaaaaaaa gaaaaagaca ctgagtgaca atcagatgct cagggtttac
      121 ctggttgtgc agaggtttgc agaagcggaa gagcagaaca tggtgaatag tcgtcgacgc
      181 aatgctatat accctgcctg gtactggctg acagttctgg atgttctgcg ttataccgga
      241 atgcgcttta atcaactgaa gcatatccgt gtgagggatg t
//
LOCUS       DAZGAP010000659          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZGAP010000659 DAZGAP010000000
VERSION     DAZGAP010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.667"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>281
                     /locus_tag="ACHLAM_005673"
     CDS             <1..>281
                     /locus_tag="ACHLAM_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7856997.1"
                     /translation="MTEKDGLCNPFPPNCIESAHPFTPFQQNQWIELDTVGSDGPVKA
                     TFLVHSVLHKLKRQGVSLNDAGRPEYGRCIAHWHTDVFVEKKALPDWLR"
BASE COUNT           64 a           71 c           77 g           69 t
ORIGIN      
        1 tcatgacgga aaaagacggg ctctgtaacc cctttccccc gaactgtatt gaatcggctc
       61 atccttttac tccgtttcag cagaatcagt ggattgaact ggacactgtc gggtcggatg
      121 gaccggtgaa agcgacgttt cttgtgcaca gcgttctgca taaactgaaa cgtcagggtg
      181 tctccctgaa tgatgccggc cggcctgaat acggccggtg cattgctcac tggcacactg
      241 acgtgtttgt tgagaaaaaa gcgctgcctg actggctgag a
//
LOCUS       DAZGAP010000660          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZGAP010000660 DAZGAP010000000
VERSION     DAZGAP010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.668"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..100
                     /locus_tag="ACHLAM_005674"
     tRNA            24..100
                     /locus_tag="ACHLAM_005674"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHLAM_005675"
     tRNA            143..218
                     /locus_tag="ACHLAM_005675"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZGAP010000661          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZGAP010000661 DAZGAP010000000
VERSION     DAZGAP010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.669"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>270
                     /locus_tag="ACHLAM_005676"
     CDS             <1..>270
                     /locus_tag="ACHLAM_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7856998.1"
                     /translation="IEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFS
                     ITLTRCGSDVGNAYIKFTGNTVSEDASLYKLEDGSVDGLALTIFDK"
BASE COUNT           81 a           43 c           65 g           81 t
ORIGIN      
        1 attgaagggc taattaccga aaaaacctgc attatttctg atgagtcaaa aaattttaca
       61 gttaatatgc cagacgtacc cagtagttcg gtaaggagtg caggggatgt tactgaaaag
      121 gtttattttt ccataacgtt aacccgctgt ggtagtgatg ttggcaacgc gtatataaag
      181 tttaccggca atacagtttc tgaagatgcc agtttatata agctggaaga tggctcggta
      241 gatgggcttg cactcacgat ttttgataag
//
LOCUS       DAZGAP010000662          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZGAP010000662 DAZGAP010000000
VERSION     DAZGAP010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.670"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>270)
                     /locus_tag="ACHLAM_005677"
     CDS             complement(<1..>270)
                     /locus_tag="ACHLAM_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO7856999.1"
                     /translation="AFFSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESI
                     QQHVPLVRDAEFRSLLRHPPAGSRVIASKDFGFALDIFFCRMMAN"
BASE COUNT           87 a           54 c           65 g           64 t
ORIGIN      
        1 ttgtttgcca tcattcgaca gaagaaaata tctaaagcga agccaaaatc cttactcgcg
       61 ataacgcgac tccctgcagg aggatggcga agtaatgatc tgaactccgc gtcccggact
      121 aagggaacat gctgctgtat ggattcaacg gaggagtaat gtttaatcag attgactttg
      181 ccttttccat ttctcagtga atcaagggcg tattgcaatg acagatggcg acaaaaaata
      241 gcgtcagaag gagattgcga aaaaaatgca
//
LOCUS       DAZGAP010000663          263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZGAP010000663 DAZGAP010000000
VERSION     DAZGAP010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.671"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>263)
                     /locus_tag="ACHLAM_005678"
     CDS             complement(<1..>263)
                     /locus_tag="ACHLAM_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO7857000.1"
                     /translation="RAYDERDRLVSETSRSGDVIRYAYDNPHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGVSLYR"
BASE COUNT           47 a           87 c           74 g           55 t
ORIGIN      
        1 gcggtaaaga ctgacacctt cctcacggtg gaccgccgtc atctggccga agcggtcgta
       61 ttcatagcgg gtctggtagc ccgagcagtc ggtgaacgcc agcaactgac cgtagcggct
      121 ccacgtcatc tgccgggtgc tgcccgtcgc atctgttgtc gtggccggta actcactgtg
      181 cggattatca taagcatacc ggatgacgtc cccactgcgc gatgtctccg ataccagcct
      241 gtcccgctca tcatacgccc ggc
//
LOCUS       DAZGAP010000664          263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZGAP010000664 DAZGAP010000000
VERSION     DAZGAP010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.672"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>263
                     /locus_tag="ACHLAM_005679"
     CDS             <1..>263
                     /locus_tag="ACHLAM_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7857001.1"
                     /translation="YLLKQWDALNEYCRNGWVEIDNNLCENALRVVALGRRNYMFFGS
                     DGGGESAAVMYSLIGSCKLNGIEPETWLRHVISVINTWPANRV"
BASE COUNT           65 a           65 c           81 g           52 t
ORIGIN      
        1 tatctgctga agcaatggga cgccctgaac gaatactgcc gcaatggctg ggtggagatc
       61 gacaataatc tgtgtgaaaa cgccctccgg gtagttgcac tggggcggcg taactacatg
      121 ttcttcggct ctgatggagg aggcgagagt gcggcagtga tgtacagcct gatcggtagc
      181 tgcaaactta acggaatcga gccggaaacg tggctacgcc acgtgatcag tgtcatcaac
      241 acctggccag ccaaccgcgt gaa
//
LOCUS       DAZGAP010000665          262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZGAP010000665 DAZGAP010000000
VERSION     DAZGAP010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.673"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>262
                     /locus_tag="ACHLAM_005680"
     CDS             <1..>262
                     /locus_tag="ACHLAM_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7857002.1"
                     /translation="YIIQDSIGLKGGWNLYQYPLNPVQHVDPLGLSTMIIGNGPVPDN
                     PFGHAAAANRYGLMSSGTGDEMGASVSDYFKKMQPRRDTWIW"
BASE COUNT           68 a           51 c           73 g           70 t
ORIGIN      
        1 ggtatatcat ccaggactcg attgggctga aggggggatg gaatctgtac caatatccgc
       61 tgaatccagt gcaacacgtt gatccattag ggcttagtac tatgataata ggtaacggcc
      121 ccgttcctga taatcctttt gggcatgctg cggcggcaaa tcgatatgga ttaatgagtt
      181 cgggaactgg ggatgagatg ggagcctctg tctctgatta ttttaaaaag atgcaacccc
      241 gcagagatac ctggatttgg at
//
LOCUS       DAZGAP010000666          259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZGAP010000666 DAZGAP010000000
VERSION     DAZGAP010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.674"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>259
                     /locus_tag="ACHLAM_005681"
     CDS             <1..>259
                     /locus_tag="ACHLAM_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO7857003.1"
                     /translation="YTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSA
                     SYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKG"
BASE COUNT           89 a           53 c           50 g           67 t
ORIGIN      
        1 ttataccctg aaaaacgaac ttcatgatcg tagtcatcga aagtatcagc aaaccattac
       61 cgataaaaca gtattgattc actatacagg gataactaaa ccatggcata gctgggctgg
      121 atatccatct gcatcatact ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc
      181 tcttaaagag gcgcggactg ttgcagaaat gcagaaacaa tataagcatc tgtttgccca
      241 tggtgagtat attaaaggt
//
LOCUS       DAZGAP010000667          258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZGAP010000667 DAZGAP010000000
VERSION     DAZGAP010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.675"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>258)
                     /locus_tag="ACHLAM_005682"
     CDS             complement(<1..>258)
                     /locus_tag="ACHLAM_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO7857004.1"
                     /translation="ETPMVLTLDQLRPNPDNPRTTRNPRYDDIKNSIHARGLDTVPKV
                     TRMPDSEPDVYIFSDGGNTRYQILTELWKETGDPRFYRIHVL"
BASE COUNT           54 a           68 c           71 g           65 t
ORIGIN      
        1 agcacatgaa tacggtaaaa acgcggatcc cctgtttctt tccagagttc cgtcagtatc
       61 tggtaacggg tgtttccccc gtcactgaag atatacacat ccggctcact gtccggcata
      121 cgggtgactt tgggcaccgt atccagcccg cgggcatgaa tggaattttt gatatcgtca
      181 taacgcggat tgcgcgtggt gcgcgggtta tccggattcg gacgtaactg gtccagcgtc
      241 agaaccatgg gcgtttcc
//
LOCUS       DAZGAP010000668          256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZGAP010000668 DAZGAP010000000
VERSION     DAZGAP010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.676"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..240)
                     /locus_tag="ACHLAM_005683"
     CDS             complement(<1..240)
                     /locus_tag="ACHLAM_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7857005.1"
                     /translation="MLLAVFIYLIRRNKLHKALRFYDESQKYELPFGYLPGAFSVLRI
                     ALEIKVNREKIKHGSLFELLDYVKAYQDAFMDLRVV"
BASE COUNT           82 a           58 c           46 g           70 t
ORIGIN      
        1 cacgaccctt aagtccataa atgcatcctg ataagctttt acataatcaa gtaattcgaa
       61 cagtgaacca tgctttattt tctccctgtt tactttgatc tccagcgcta tcctgagaac
      121 tgaaaaagcg ccgggaagat acccaaaagg taactcgtat ttttgggatt catcataaaa
      181 ccgaagagct ttgtgaagtt tgttccgccg aatcagataa ataaaaacgg ccagtaacat
      241 gtagcacctg gtcaca
//
LOCUS       DAZGAP010000669          253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZGAP010000669 DAZGAP010000000
VERSION     DAZGAP010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.677"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>253
                     /locus_tag="ACHLAM_005684"
     CDS             <1..>253
                     /locus_tag="ACHLAM_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO7857006.1"
                     /translation="QILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQNEIKNQHPL
                     PEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSN"
BASE COUNT           81 a           47 c           50 g           75 t
ORIGIN      
        1 acagattttg tatacatact ctgatgctgg caattcagtc tttctggtcg ttgatcatct
       61 gccatggact gacagcgata aaataaattg gtatctgaag catcagaatg aaattaaaaa
      121 tcaacatccc ctaccagaag gtagttggca tacctggtat gttattgata tcggtaatgg
      181 cttcactgac tataaaaaat atatcgaagg gccgtatgaa gatctctact gtttcccgac
      241 gattaagagc aat
//
LOCUS       DAZGAP010000670          253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZGAP010000670 DAZGAP010000000
VERSION     DAZGAP010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.678"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>253)
                     /locus_tag="ACHLAM_005685"
     CDS             complement(<1..>253)
                     /locus_tag="ACHLAM_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7857007.1"
                     /translation="GAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAG
                     TISFLSVKISDNGVFLGTTLSISCLILALLSMFVTIIIGN"
BASE COUNT           87 a           45 c           43 g           78 t
ORIGIN      
        1 tattccctat aataatagtt acgaacattg acaataatgc aagaataaga caactaatac
       61 ttaaagtggt tcccaaaaaa actccattat cgcttatttt tactgaaagg aaactgattg
      121 tccctgcgct tactgtttga gtaaagcctg taattgatgc tgctaaacca gttcgtgaac
      181 cgtttatctg caatgagatg aaattagcta ttggatagta tgcgccaaga gttaaattga
      241 aaatcattgc tcc
//
LOCUS       DAZGAP010000671          249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZGAP010000671 DAZGAP010000000
VERSION     DAZGAP010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.679"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..201)
                     /locus_tag="ACHLAM_005686"
     CDS             complement(<1..201)
                     /locus_tag="ACHLAM_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7857008.1"
                     /translation="MREISDNMLDSVKGGMNLNGLPGSTNVIDLRGKDMGTYIDANGA
                     CWAPDTPSIIIYPGGSGPSYSMS"
BASE COUNT           68 a           58 c           39 g           84 t
ORIGIN      
        1 actcatacta taagagggtc cacttccccc cggatatatg atgatggatg gagtatccgg
       61 agcccagcat gctccattag catcaatata tgttcccata tcttttccac gtagatctat
      121 tacattagta gaaccaggta atccattaag attcatccct cctttcacgg aatcaagcat
      181 gttatctgat atttctctca ttttaaaaac ctcctgctca tttatgttca gatattaatt
      241 tggtgttgc
//
LOCUS       DAZGAP010000672          249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZGAP010000672 DAZGAP010000000
VERSION     DAZGAP010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.denovo.680"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>249)
                     /locus_tag="ACHLAM_005687"
     CDS             complement(<1..>249)
                     /locus_tag="ACHLAM_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7857009.1"
                     /translation="EAGGEFSGEITGVTDGAGRHFRLVLTTQALRAEEARQKATSGGT
                     EPSAFPDTQPDYTEYGRDNGIRLSAVWLTHDPEYPENLP"
BASE COUNT           38 a           82 c           79 g           50 t
ORIGIN      
        1 ggtaaattct ccgggtattc cgggtcgtgc gtcagccaca cggcagacag acggataccg
       61 ttgtcccggc catattccgt gtagtccggc tgggtatcag gaaaagcgga cggttccgtc
      121 ccgccggaag tggccttctg ccgggcctct tctgcccgca gcgcctgcgt ggtcagtacc
      181 agccggaagt gacggcctgc gccgtcggtc acgccggtga tttcgccgct gaattcgccg
      241 ccagcctca
//
LOCUS       DAZGAP010000673        17815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.guided.110, whole genome shotgun sequence.
ACCESSION   DAZGAP010000673 DAZGAP010000000
VERSION     DAZGAP010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.guided.110"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHLAM_005688"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHLAM_005688"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO7857010.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHLAM_005689"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHLAM_005689"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO7857011.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHLAM_005690"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHLAM_005690"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO7857012.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHLAM_005691"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHLAM_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO7857013.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6384..6698)
                     /locus_tag="ACHLAM_005692"
     CDS             complement(6384..6698)
                     /locus_tag="ACHLAM_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO7857014.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6695..7009)
                     /gene="yjeN"
                     /locus_tag="ACHLAM_005693"
     CDS             complement(6695..7009)
                     /gene="yjeN"
                     /locus_tag="ACHLAM_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO7857015.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(7072..8574)
                     /gene="yjeM"
                     /locus_tag="ACHLAM_005694"
     CDS             complement(7072..8574)
                     /gene="yjeM"
                     /locus_tag="ACHLAM_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO7857016.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8793..9770)
                     /gene="epmA"
                     /locus_tag="ACHLAM_005695"
     CDS             complement(8793..9770)
                     /gene="epmA"
                     /locus_tag="ACHLAM_005695"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO7857017.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10095..11903
                     /gene="frdA"
                     /locus_tag="ACHLAM_005696"
     CDS             10095..11903
                     /gene="frdA"
                     /locus_tag="ACHLAM_005696"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO7857018.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11896..12630
                     /gene="frdB"
                     /locus_tag="ACHLAM_005697"
     CDS             11896..12630
                     /gene="frdB"
                     /locus_tag="ACHLAM_005697"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO7857019.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12641..13036
                     /gene="frdC"
                     /locus_tag="ACHLAM_005698"
     CDS             12641..13036
                     /gene="frdC"
                     /locus_tag="ACHLAM_005698"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO7857020.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13047..13406
                     /gene="frdD"
                     /locus_tag="ACHLAM_005699"
     CDS             13047..13406
                     /gene="frdD"
                     /locus_tag="ACHLAM_005699"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO7857021.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13469..14602
                     /locus_tag="ACHLAM_005700"
     CDS             13469..14602
                     /locus_tag="ACHLAM_005700"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7857022.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            14691..15224
                     /gene="blc"
                     /locus_tag="ACHLAM_005701"
     CDS             14691..15224
                     /gene="blc"
                     /locus_tag="ACHLAM_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7857023.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15225..15542)
                     /gene="sugE"
                     /locus_tag="ACHLAM_005702"
     CDS             complement(15225..15542)
                     /gene="sugE"
                     /locus_tag="ACHLAM_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7857024.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15717..15863)
                     /gene="ecnB"
                     /locus_tag="ACHLAM_005703"
     CDS             complement(15717..15863)
                     /gene="ecnB"
                     /locus_tag="ACHLAM_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7857025.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15974..16099)
                     /gene="ecnA"
                     /locus_tag="ACHLAM_005704"
     CDS             complement(15974..16099)
                     /gene="ecnA"
                     /locus_tag="ACHLAM_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7857026.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(16151..16717)
                     /gene="efp"
                     /locus_tag="ACHLAM_005705"
     CDS             complement(16151..16717)
                     /gene="efp"
                     /locus_tag="ACHLAM_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7857027.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16759..17787
                     /gene="epmB"
                     /locus_tag="ACHLAM_005706"
     CDS             16759..17787
                     /gene="epmB"
                     /locus_tag="ACHLAM_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7857028.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4450 a         4697 c         4582 g         4086 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagttct gccaccgact
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcata tatttaaaca ggaagcgacg atcctggccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc caccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccagc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttttacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcattcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcatt gctgggtctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 gtctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgactc cccaggagtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactact taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaaggaaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gatatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggctggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacaccaga cgctgaaccg gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaacccatt ggttgacgac aacaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atctcacaag
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccgga agtcgtagag gcgctccagt
     3181 ccgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgaacc gcgcagcgtg tcaccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggaacgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttaaatga aatcgagcgc cgcctgggga cgcttaccgg caattccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtc gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gctcgtccaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct aaataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtgga gacacgctac tactggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaatgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgta atgatgctga
     4441 ccagtaaaga gacgattttg ccgctgcttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgggtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt cactctcagt ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccgctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca gcgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattctgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtaa tcgataaccc agcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt caaattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc actacgccat gagattcacc agctaattct ggcaggattc catgcccacg
     6241 gtattgatat gccattcccg cccttccaga tgcgtttgga aagcctgaat ggtaagcaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taaggtcttt
     6361 gtggccggat gaacccatcc ggcttataag caaaactgat agcgcagaaa aaactgccac
     6421 agccaatagc cgaaaatagc cagcgcggtg gcgtacataa accagccgcg tttttttcgc
     6481 cacaaagcga accagagaaa agggaaaaag agcggtaacg tcattatcac accgacatca
     6541 cgggtatcgt catcttcgag atacatcagc acatcgcaga cgttattaat cccgcgtcca
     6601 tcaatcatcc actctttgtc cagcgcggaa gtgatggtta tccagaggaa cgccacaata
     6661 aaccagatgc agcacaagat aaacatgcgc gcactcatga gataatcggt cgcttaattt
     6721 cgctatagcg catacgaaac aaatcgccgt atcccactaa ttctccgcga aaaaccagac
     6781 atctaataag ataagcgcaa tagatatccg gcaggtttcc ccaccgcgga ccatcgcgct
     6841 ctttgagggt attaatagtg ttacccacca ccatcgctac tttgcgggtg tggcgggtat
     6901 cgacaaaaga gaggaccgtt tgctctattt ccgccacatc cccggcagaa aattgcagct
     6961 ccctgatttc atcttcggtt atcactggat cccgactagc gtcgtccata acgtcttcct
     7021 ttccagacaa ctcatcgccg gagagacgat gagttgcagg atttcatatg atcagtcgag
     7081 agctaactca ggtttgttcg ccatgcgact gcaatagttc tggtaaatcg ccatcgctaa
     7141 cagcgagaag aagacagggc cgccaatcat ccacaatgtg ctgtcccagt ctccagcttc
     7201 aaccacaggt tgaatgatgg tgaagacgtt cgcaaatgtc accaccagta cgacaaccac
     7261 tgttgcaatc attgccgaca tatgcgtttt gaaaatcaca aacggtctgt cgagatcctg
     7321 acgtgcttta aagaatggga aagccagcgc gaggaacagg taaggaagcg tcatagacac
     7381 gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg gtaccgccac caaacgaaac
     7441 cagcaggatg aagacagtaa ccaacccgca ctgcatccac atggcgatag acggcatccc
     7501 catcgcattc aggcgcgtca tcggttccgg ccacaacgct ttcggcgtcc cctggatgat
     7561 ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg gtataagcga ggaacatcga
     7621 aagtccggta atacgcgcaa accatacgcc cagcgacaat gacgcttcag gtgacaaatg
     7681 cagtgcgtta cccagcgtca ccccgaggct cttcatcagc acataggtaa tattgccgag
     7741 gttaacggaa ccattactta atacctgctg ccagtttgtg ctgacgcccc ataaaaatat
     7801 tgccagcgaa taaccgattg aaataacaat agcggcaaaa acaatacctt tggcaaagtt
     7861 cttttctgga ttttccgttt tatcgaccag accaccaacc gcttcaattc caccataggc
     7921 aaaaatagca aataccacaa acgatagcat tgccagaccg gactgataac ctgggttcgg
     7981 tgatgcaagg aaattaatat cctgcgcgaa atgcccacca tttaataaca aaatagtaat
     8041 gcttactaac agcaatacta aattcagaca cattactgca ataccgccca ccgcagtaat
     8101 gcgggcaatt ttattaatcc cttttgaagc aacgacggtg accagaatca tccatgccac
     8161 tgccagcaga ccaaccacct gcgtaggctc cagtccggca atacgccagt gctgggtcat
     8221 gtcgctacca tagaggaatg ttgagaacgg tacccaaact ttcgcggagg tactcaccat
     8281 ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac gtggtccgac
     8341 actattattc atccaggaat agataccgcc ttcttctttg cgataagcag ctcccatttc
     8401 agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat
     8461 cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt
     8521 aaagatcatc aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaaac
     8581 atccttcatt tcagcgcgtg tataaaacgc gcccactgaa ctgcactgcc caacattgag
     8641 cgggcagcgt aaaaaccaat acagaattga tacctggatg atatcaaata tccagtctta
     8701 aattgtggcc tttagcggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca
     8761 ttcttcgcgg ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg
     8821 acttcagcca gtgtctccgc gcccagcgcc aacatcacca gacgatcaac gcctaatgcc
     8881 acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg
     8941 tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc
     9001 tcacgggcat ccgtcaattc atggaaacca ttcgccagct caataccttt ataataaacc
     9061 tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg
     9121 gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag
     9181 gtaaacagca attgtagaag cgtgtcgcgg tcttcttcag tatcggcaac gttgctcaaa
     9241 tccagtttcg ctgccacttc ccgcagttgc gttttgtcgg cagagagtgg gtcaatttcc
     9301 agataacgca agaaagcttg ttgataagaa aggctttctg ctgccggaca gtccagcacc
     9361 tgttgcaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac
     9421 cactcaagca tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg
     9481 cggcacagct ggaataccgg cccacaacca gcaaccagca ggcgtttcat atggtattcc
     9541 gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggcctac gaaacgtgtc
     9601 tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc
     9661 tccagcactc cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt
     9721 aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct
     9781 ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc
     9841 aggaatcaaa caagggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta
     9901 aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca
     9961 aatttcagac ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg
    10021 ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa
    10081 tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg
    10141 cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa
    10201 tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg
    10261 tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact
    10321 ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgccccacc gaaatgaccc
    10381 aactggaact gtggggatgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc
    10441 gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata
    10501 tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac
    10561 atttcgtact ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca
    10621 tgatggaagg cacgctggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg
    10681 cgggtcgcgt ttatcgttac aataccaatg gcggcatcgt taccggtgac ggtatgggta
    10741 tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg
    10801 gtctgccagg ttccggtatc ctgatgaccg aaggctgccg cggtgaaggc ggtattctgg
    10861 tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg
    10921 gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct
    10981 ggcacgaatg gcgtaaaggc aacactatct ccacgccgcg tggcgatgtg gtttatctcg
    11041 acttgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg
    11101 cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac
    11161 actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt
    11221 tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggttctaact
    11281 ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag
    11341 caactgccgg taatggcaac gaagcggtaa ttgaagcgca ggcagctggc gttgaacaac
    11401 gtttgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa
    11461 tgggcctggc tatggaagaa ggctgcggta tctaccgtac gccggaactg atgcagaaaa
    11521 ccatcgacaa gctggcagag ctgcaggaac gcttcaaacg cgtgcgcatc accgacactt
    11581 ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg
    11641 ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc
    11701 tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc
    11761 gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc
    11821 cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata
    11881 agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac
    11941 ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc
    12001 tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct gagctaccgc
    12061 tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa
    12121 ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct
    12181 aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa
    12241 gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag
    12301 accccggcgc agatggccaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc
    12361 tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg
    12421 ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag
    12481 ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctactgctc cgaagtctgc
    12541 ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac
    12601 tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc
    12661 gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttctacat
    12721 gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct
    12781 gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc
    12841 ggttatcgtg atcattaacc tgatcactct ggcggcagcc ctgctgcaca ccaaaacctg
    12901 gtttgagctg gcaccaaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga
    12961 gccaattatc aaaagcctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt
    13021 tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac
    13081 cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga
    13141 tgatcctgct ggttggtatt ctgctgccac tggggctgtt tccgggtgac gcactgagct
    13201 acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga
    13261 tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa
    13321 tccacgtacc tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca
    13381 cgctgattgg tgtcgttaca atctaacgca tcgccaatgt aaatccggcc cgcctatggc
    13441 gggccgtttt gtatggaaac cagaccctat gttcaaaatg acgctctgcg ccttattaat
    13501 taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac
    13561 aattaccccg cttatagagc aacaaaagat ccccggtata gcggtggcgg taatttatca
    13621 gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac
    13681 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
    13741 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcgacaaaat actggcctga
    13801 acttaccgct aaacaatgga atgggatcac actattacat ctcgcgacct acaccgctgg
    13861 cggcctgcca ttgcaggtgc cggatgacgt taaatcctca agcgacttgc tgcgcttcta
    13921 tcaaaactgg caacctgcat gggctccagg aacacaacgt ctgtatgcca acgccagtat
    13981 tgggttgttc ggtgcactgg cggtgaaacc atccggtttg agctttgaac aggcgatgca
    14041 aactcgtgtt ttccagccac tcaaactcac tcatacgtgg attaatgtgc cgtccgcaga
    14101 agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc
    14161 gttagatgct gaggcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca
    14221 aagcaattta aaaccccttg atatcactga gaaaacgctt caacaaggga tacaactggc
    14281 acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga
    14341 ctggccggtc aatcctgaca tcatcattaa cggcagtgac aataaaattg cactggcagc
    14401 acgccccgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa
    14461 aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
    14521 tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca
    14581 gattctcaac gctctacagt aaaatttcat cgggtccgaa tttttggacc ttttctccgc
    14641 ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc
    14701 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
    14761 cgccccgtgg cgtgaccgta gtgaataatt tcgatgctaa acgctatctc ggtacctggt
    14821 atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat
    14881 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
    14941 aaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
    15001 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
    15061 aataccgcca tgcgctggtt tgcggtccgg accgcgacta cctgtggata ctctcccgca
    15121 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
    15181 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagttagtg agtgctgagt
    15241 ttcagaccaa taatccccaa tacgattaac gccagactcg ccaggcgcat cgggttagcg
    15301 gactcgccga gcagcacaat gccggtgatg gccgcgccga ctgcgccaat acccgtccac
    15361 acggcataag ccgtccctac tggtaacgat ttcatcgccc aggcaagtag cgccatactg
    15421 acaatcatcg ccgtcacagt aataacactt ggcgtcaaac gactaaagcc gtgggtatat
    15481 ttcaggccaa cggcccatac cacttccagc aggccagcaa taactaagat aatccaggac
    15541 atatcaggct ccggagaatt ggggccgtcc ccggtgaaaa gaggcgtttg caggtcgtcc
    15601 tgcaaagctg atggttgaac ggatattttg ccaggtggca ggggaatttc aactattacc
    15661 tttgtgacta tcgcaaaaaa acaaacggca cgacacagct gtcgtaccgt tattgcttac
    15721 tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca
    15781 acgccacgcg tggtgttgca agcagttaat actgttgaaa gcaccagaac agaaaagatc
    15841 gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc
    15901 tatagcaaat taataataga caacatcgcc aaaaatgatg gaaatctgat gatttttcgg
    15961 aggagaagaa aaattagctg gcagcgcggg agatggagtt gccgagatgt ttaatgtctt
    16021 caccaaaacc gcgagcggtg ttacagcccg tgagcagcgc actggcaagc aaaacaagaa
    16081 cgataaggcg tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac
    16141 cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga
    16201 cttcgccgat ctgtacgaac agcggaactt taaccacagc gccagtagac agggtagctg
    16261 gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca
    16321 gctcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac
    16381 actctgcctg atccagcaac catttggcgt tgtcgccaat tgcttttgca tcagcagaca
    16441 gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag
    16501 tcaggttcat atcgacaaca tcagcacctt cagcggaatc agtagatttg aaggtttttt
    16561 ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac
    16621 ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa
    16681 gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat
    16741 tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat
    16801 tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat
    16861 atagacgcgg acgaaaaact gttggccggg cgcagcgcca aaaagctgtt tgccctgcgt
    16921 gtgccccgct catttatcga tcgcatggag aaaggcaatc cgaacgatcc gcttttgcgt
    16981 caggtactta cctcgcaaga tgagtttgtt gtcgcgcccg gattctccac cgacccgctg
    17041 gaagaacagc acagcgtagt gccaggtttg ttgcataaat accacaaccg ggcgcttttg
    17101 ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat
    17161 gccgaaaatc agggcaacaa gcgtaactgg cagactgcac ttgagtatgt tgctgcgcat
    17221 ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag
    17281 ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac
    17341 agccgtctgc cgattgtgat cccggcacgc atcacagatg cgctggttga acgcttttct
    17401 cattctacgc tgcaaatctt gctggtgaat cacattaacc atgccaatga ggtagatgaa
    17461 acattccgtc aggcgatggc taagttgcgc cgggtaggcg ttactttgct caaccagagc
    17521 gttctgttac gtggtgtgaa cgataacgcg caaacgctgg caaacctgag taatgcgttg
    17581 ttcgatgcgg gcgtgatgcc gtattacctg catgtgctcg ataaagtaca gggcgcggcg
    17641 cattttatgg tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacactggtg
    17701 tcgggatatc tggtgcctaa actggcgcga gaaattggcg gcgaacccag caaaacgccg
    17761 ctggatctcc agctacgcca gcagtaactg attaaacagg gaagtttaat ctttg
//
LOCUS       DAZGAP010000674         4891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.guided.277, whole genome shotgun sequence.
ACCESSION   DAZGAP010000674 DAZGAP010000000
VERSION     DAZGAP010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.guided.277"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..326
                     /locus_tag="ACHLAM_005707"
                     /pseudo
     CDS             120..326
                     /locus_tag="ACHLAM_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            322..674
                     /gene="prfH"
                     /locus_tag="ACHLAM_005708"
                     /pseudo
     CDS             322..674
                     /gene="prfH"
                     /locus_tag="ACHLAM_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHLAM_005709"
     CDS             complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHLAM_005709"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7857029.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2449..2907
                     /gene="gpt"
                     /locus_tag="ACHLAM_005710"
     CDS             2449..2907
                     /gene="gpt"
                     /locus_tag="ACHLAM_005710"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7857030.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2999..4243
                     /gene="frsA"
                     /locus_tag="ACHLAM_005711"
     CDS             2999..4243
                     /gene="frsA"
                     /locus_tag="ACHLAM_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7857031.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4301..4702
                     /gene="crl"
                     /locus_tag="ACHLAM_005712"
     CDS             4301..4702
                     /gene="crl"
                     /locus_tag="ACHLAM_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7857032.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(4741..>4891)
                     /locus_tag="ACHLAM_005713"
     CDS             complement(4741..>4891)
                     /locus_tag="ACHLAM_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7857033.1"
                     /translation="VNYVDVGATYYFNKNMSAFVDYKINQLESDNKLNINNDDIVAVG
                     MTYQF"
BASE COUNT         1199 a         1193 c         1276 g         1223 t
ORIGIN      
        1 tgcaggaagg cgacaagcga gtgaatcccc aggagcttac taaagtaagt gactggggtg
       61 aacgaacgcg gtcgcagtac atgcaacttg aagtatgacg agtataaatg gaatgataca
      121 tgggcaaata tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt
      181 ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc
      241 gcgttgtggg gatgccagat ttacatcccg gacgcggcta cccgattggc gcagcgttct
      301 tctctgttgg tcgtttttac ccggcaagac gtcgcggtaa cggtgctgga aacagaaacg
      361 ggccgctact ctgacacgct gcgttcggcg ctgatttctc tggatggcga taatgcatgg
      421 gcattaagcg aaagttggtg cggcactatt cagtgtattt gtccgagtcc gtatcggcct
      481 catcatgggc gcaaaaactg gtttctgggc atgctaacaa gcggctggcg cgattgctga
      541 ttgcctggaa gctggaacaa cagcaacagg aaaatagcgc ggcgctgaaa tcgcagcggc
      601 gaatgttcca tcaccagatt gaacgtggca acccgcgacg aacgttcaca gggatggctt
      661 ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca
      721 aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagtagt gtccagtaat
      781 gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg atggttggcc
      841 cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg
      901 cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca
      961 gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg
     1021 ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag tctttaccgc
     1081 tgtcgatcag agaacggatc aggcagtgaa tttctacgtt attgtcagtc atggtcacca
     1141 caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggagttg cggatcacac
     1201 cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag
     1261 ctttatcgtt cgcaacagag tccagcaaca aggccagatt tttctctttc tctgccagct
     1321 cgtttttcag gatctcctga taggtattca ccagagattt caaagcgtcg actttatcag
     1381 ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt
     1441 tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca
     1501 gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcaggc
     1561 cttttaaggt taacttgaag gtttcaaagc cagctggaac cgcttcacga tctaaatgca
     1621 ggttggaggt gaagtcgata cctcccgcac aacccatgta gatttcacct tcttcttcgg
     1681 agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagcca aaagcaccgt
     1741 ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt
     1801 cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg
     1861 tgccgcgtgc tttaacccat tcgccatcaa tataaggctg gataggatct ttggtgaagt
     1921 catgcacggt gtcgttattt ttttgcggca ccatatcgag gtgggcctgc aagacgaccg
     1981 gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat
     2041 cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct
     2101 cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct
     2161 gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca
     2221 gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata
     2281 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     2341 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     2401 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     2461 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     2521 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg
     2581 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     2641 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     2701 atcgttattg atgacctggt agataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     2761 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgat
     2821 tatgttgttg atatcccgca aaatacctgg atcgaacagc cgtgggatat gggcgtcgta
     2881 ttcgtgccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     2941 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     3001 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     3061 gacgctagtc cgccgcttta gtcacggcgc acaactgcct gtgcagtcgg cccttgatgg
     3121 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     3181 tgacccccgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg ccgaacgtac
     3241 cgacgatgat ttatacgata cggtgattgg ctaccgcggc ggcaactgga tttatgagtg
     3301 ggctacccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag
     3361 cggtcgatac tggctgcatg cggctacgtt gtacaacatt gctgcctatc ctcatctgaa
     3421 aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgcgcctatg aagaggccgc
     3481 tcagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat
     3541 caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg
     3601 tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg
     3661 cggcattgct atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct
     3721 cacccaggac tccagcctgt tgcatcagca cgttttaaag gcgctgccta acgtaccgtg
     3781 ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg
     3841 tctggcatac cttgaatcgc cgcgtctgaa agtggttgcc tgtcttggtc cggtagttca
     3901 taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct
     3961 ggccagtcgt ttagggatgc atgatgcttc cgatgaagcg ttgcgagtgg agttgaatcg
     4021 ctattcatta aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa tgttatcagg
     4081 ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca cgtcgtcatc
     4141 tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg
     4201 ccttcaggaa atcacagact ggatcgaaaa acgtttgtgt taaaaatttg ctaaatttta
     4261 ccaatttggt aaaacagttg catcacaaca ggagattgca atgacgttac cgagtggaca
     4321 cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa
     4381 gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc
     4441 accggaagtg cgtgagttct ggggctggtg gatggaactg gaagcgcagg aatcccgttt
     4501 tacctacagt taccagtttg gtctgttcga taaagcaggc gactggacga gtgttcagat
     4561 aaaagatgcc gaagtggttg aacgactgga gcacaccctg cgtgaatttc acgagaagct
     4621 gcgtgagctg ctggcgacgc tgaatctgaa gctggaaccg gcagatgatt ttcgtgatga
     4681 accggtgaag ttaacggcgt gaatgaaatg tgccggatgc atcacatccg gcaatattca
     4741 ttaaaactga tacgtcatcc caaccgcgac aatatcatca ttattaatat ttaatttgtt
     4801 atcgctttcc agttggttga ttttataatc aacaaacgct gacatatttt tgttgaaata
     4861 atacgtagca ccaacatcga cataattgac c
//
LOCUS       DAZGAP010000675         1465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2301M18102
            SAMN43283619-rid23083683.guided.414, whole genome shotgun sequence.
ACCESSION   DAZGAP010000675 DAZGAP010000000
VERSION     DAZGAP010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283619
            Sequence Read Archive: SRR30325127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351389.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351389.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:26:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 91
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 77 of 182
            Pseudo Genes (incomplete)         :: 125 of 182
            Pseudo Genes (internal stop)      :: 46 of 182
            Pseudo Genes (multiple problems)  :: 53 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283619-rid23083683.guided.414"
                     /serotype="O146:H28"
                     /isolate="2301M18102"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHLAM_005714"
     CDS             132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHLAM_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO7857034.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHLAM_005715"
     CDS             1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHLAM_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO7857035.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          401 a          296 c          353 g          415 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tacttatttt ttctggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aatacctgta tatgaagtgt atattgttaa aatgggtact gtgcctgtta ctgggctttt
      181 cttcggtatc ctattcccgg gaatttacga tagacttttc gacccaacaa agttatgtct
      241 cttcgttaaa tagtatacgg acagaaatat cgaccccact tgaacatata tctcagggga
      301 ccacatcggt gtctgttatt aaccacaccc caccgggcag ttattttgct gtggatatac
      361 gagggcttga tgtctatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggccggattc gttaatacgg caacaaatac tttctacagg ttttcagatt
      481 ttgcacatat atcagtgccc ggtgtgacaa ctgtttccat gacaacggac agcagttata
      541 ccactctgca gcgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt ttagtggaaa tgccatgacc agagatgcat
      661 ccagagcagt tctgcgtttt gtcactgtca cagcagaagc cttacggttc aggcaaatac
      721 agagagaatt tcgtctggca ctgtctgaaa ctgctcctgt ttatacgatg acaccggaag
      781 aagtggacct cacactgaac tgggggagaa tcagcaatgt gcttccggag tttcggggag
      841 agggtggtgt cagagtgggg cgaatatcct ttaataatat atcagcgata ctgggcacag
      901 tggcggttat actgaattgc catcatcagg gggcgcgttc cgttcgcgcc gtgaatgaag
      961 agatacaacc agaatgtcag ataactggcg acaggccagt tataaggata aacaatacgt
     1021 tatgggaaag taataccgca gctgcttttc tgaatcgcag ggctcactct ttaaatacat
     1081 ccggagaata acaggagtta aatatgaaga agatatttgt agcggcttta tttgcttttg
     1141 tttctgttaa tgcaatggca gctgattgtg caaaaggtaa aattgagttc tctaagtata
     1201 atgagaatga tacattcaca gtaaaagtgg ccgggaaaga gtactggact aaccgctgga
     1261 atctgcaacc gctactgcaa agcgcacagt taacaggaat gacggtaaca atcaaatcaa
     1321 atacctgtgc gtcaggttca ggatttgctg aagtgcagtt taattaatat cagaagcatt
     1381 gctggtttcg tggtgtgcag caatgtagtt acagtgtaat caatgtcaca attcagtcag
     1441 ttgaaggttg tctgcccgac tgaga
//
